LOCUS       DAOOYB010000001        45133 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_680_length_45133_cov_8.942278, whole genome shotgun sequence.
ACCESSION   DAOOYB010000001 DAOOYB010000000
VERSION     DAOOYB010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 45133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 45133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45133
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_680_length_45133_cov_8.942278"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="PL056_00005"
     CDS             complement(<1..398)
                     /locus_tag="PL056_00005"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPM45424.1"
                     /translation="MKITDKAVFETTMGKITVGLYGEDMPITVKNFIRHIDAKFYNKL
                     IFHRIIDGFVIQGGGMLKGMYEKTAMVEPIKLERNPNILHKKYTLSMARTSEPNSATS
                     QFFICTEDCPSLDNNYAAFGTVTEGMEIVDA"
     gene            548..3610
                     /locus_tag="PL056_00010"
     CDS             548..3610
                     /locus_tag="PL056_00010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HPM45425.1"
                     /translation="MSEHFAVSKLTKHVFCVLSVILLTVQSLYGLNPEQKINRFTTEC
                     FDSSKGLPQNTVADIIQSRSGYIILATQEGLVKYDGRKFTMMSENGHLNSSSRNITGI
                     FEHKNGNTVFSTAAGIIDIKEKKTLFDGVFIKDFVYDRSGVLWAGTYSSGIIRVATDG
                     SETVFTKEGGKINSNVVNSLFMSSKGKIYAATLEGVSRLEEDKFVPVDGIFAFGYRIV
                     EDKNGWIWVATERGLALIKDDKLDYIYTAEDGLKDESLRSVFADSLGSLWIGTEKGDL
                     IRFFKGKFSKLSKSNTKTGAIISIFEDREGNIWFGTEATGACIVRDGTVFQAGINTGN
                     IRSLTETADGEIWAATFGEGIYVLKKDETVTNFTMSDGLRSDSIATIFADSQSRIWVG
                     TRRNGVQIFQKGRFYDIDAFGKNFSEAHPVSPTLFFEDSRGNIWIADRHSHKPVFTWN
                     NGQMTSYEIIKGELSILDIAESRKGIVHLLSLKEGVFEFDANSGEFRNIPVKPDAKMT
                     ALFFDRQDRMWITTLSQGIIIKTEKGIVELNEANGLYSNTVHDIVQDGSGAYWFSTNR
                     GIFTISEKDAEKFISGVADRLSHRLFKEEDGMPAGECNGGSQPSVIKSSDGTLWFPTI
                     RGVVTIDPSKIEQRVELPPVTIVSAIIDNVNHIDLAARSELVVPPGTKNILINFTSLY
                     FSHPGKVRFEYMLEGFDGSWNNASANRMVNYTNIAPGVYKFLVKSYLADTPESYSETS
                     MRLEIKPFFYQDSRFRSIAAIIFLLMILAAYNLKIRIHKMREKELQKTVDERTQELLK
                     VNEKLRQSILKDPMTGLCNRRYLFEIEQPRYERMLFASKKNLQNSDGKPVEPEKVTGL
                     FLIDIVGLKKINEKRGYDFGDRLLKAFAESLKSSVRKDDLVVRWGGDEFLVILNATDQ
                     SHLPVYAKKILSMGLEGIKIEGEDPMKIVVTTGFSAMPFYPGEHTLNFEESLLMSDMA
                     LFKSLSKGPGSIKQAVPGSFVPEKEQIDSFMKDIDEGIKDGFFNIIDI"
     gene            3631..5355
                     /locus_tag="PL056_00015"
     CDS             3631..5355
                     /locus_tag="PL056_00015"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HPM45426.1"
                     /translation="MKVSQIFFQTLKEVPKEAQIESHRLLYRAGMIQKLASGIYSYLP
                     LVLRSVRKLENIIREELNKRGCQEVLMPAVQPAELWEESGRWTYYGPELLRFKDRKGG
                     DFCLGPTHEEVITDMVRKNLRSYKQLPYNLYQFQSKFRDEIRPRFGLMRGREFIMKDG
                     YSFDINEENAKKSYQLMFEAYNAIFNRCGLKFKPVDAATGAIGGDMSHEFQVLAEAGE
                     DLILSCDKCGYAANLEKARSQFVPSAEDVEELPLKEVHTPSRKAVEDVAEFLGRSSKD
                     FIKSMIYIVDDEPVLIMVPGDREVNEAKIQAFFRANSVALANDAVIAEVTGAETGFAG
                     PVGLKKKIKTAVDRLLTGRKNLVAGANKTDHHIENININRDFTTDVTGDFVVVRKGEK
                     CPDCDGTLEEYRGIEVGQVFYLGTKYSEKMNCSFVDESGKTVTAVMGCYGLGVGRTVQ
                     AAIEQNHDEFGIQWPMALAPYEAVVLPLQMNKPEVVEAAENIYKKLLEAGVETVIDDR
                     DERAGFKFNDADLVGYPLQIIFGGKSLEKGVVEVKIRKTNTKLEVKLEEIIDFTVSYK
                     KKELGLND"
     gene            5348..5944
                     /locus_tag="PL056_00020"
     CDS             5348..5944
                     /locus_tag="PL056_00020"
                     /inference="COORDINATES: protein motif:HMM:NF027363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HPM45427.1"
                     /translation="MINIVKSAITAVILSVIFFSISLHAETVDFPEKIQIDEQRSVPF
                     RNYAVFAPVSFSLYAIGLYVAGTETDNMKLKNSDEPMAIQLVSLYRRLKMKTLIDELR
                     RGFSYGMDHDKKFLKTIQERIDDFIKLLENTGKNPRKYNAITFLYVPKEGTHVSFRSE
                     YLGTIPGHDFKKALFGIWLNNNCANDELRDRVIKIRKK"
     gene            5963..6826
                     /locus_tag="PL056_00025"
     CDS             5963..6826
                     /locus_tag="PL056_00025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPM45428.1"
                     /translation="MNSNIPTLSSNTISSYIHSVLSVPDISREDEVELFKKFRENGDK
                     EAAQILVLSNLKLVVNLAYKFRKFRDVTDLIQEGNLGLMTAMQKFDIEKGVRFSTYAT
                     WWIKAKIQEFIISQMSIVRFGKSRDERKLFFNMMSTIKDIQSYDSDGEITKEDLILEV
                     AKRLDVGPEKVIEALQVVSSYNDISIDQTIEGTETKMIELRDKTDFDEEMDEETRNIR
                     LQNAIVMLNDREKFIIWNRYMSDEPMTLEEIGEKYEISKERVRQIEARALEKIKLFTS
                     DSHIIEQTGTV"
     gene            6840..7598
                     /gene="deoC"
                     /locus_tag="PL056_00030"
     CDS             6840..7598
                     /gene="deoC"
                     /locus_tag="PL056_00030"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HPM45429.1"
                     /translation="MIEQIKKRAHFLENELKNCGILRMPDFDSNYSCGDIDINLAVNA
                     IDHTVLSFNATEKDIDKACADAGKFKFKAICINPVWVKRAFDRRKKEGHEFLIATVID
                     FPLGASSLNARIAETKQAVADGADEIDIVINIGLLKSGILKETYTNLKEVIASVKSYY
                     KVILEASELTSEEKIDGALISVFAGADMLKTSTGVNGKASVEDVRILRMIAGSHLGVK
                     AAGGIRDKQTLVEMMKAGADRIGCSSSVNIINSW"
     gene            7603..8160
                     /locus_tag="PL056_00035"
     CDS             7603..8160
                     /locus_tag="PL056_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45430.1"
                     /translation="MFPIIMKRHIPNFLNYAVIPVILLFFIACSKIEKDEVGIFKEFM
                     ELRKAGNYGAAYEMLSDPTRQVFSKEAFDNYCFVYRVIDFEVFKESRGYVRSVYNFYD
                     KKYKKDSDELYTFYISSNTETVRIKEGKILFPHIGFIEMRKNIEERKTDELKETLRKM
                     IIIDPENPEVNQTAKKMELLSGSEE"
     gene            8157..9032
                     /locus_tag="PL056_00040"
     CDS             8157..9032
                     /locus_tag="PL056_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45431.1"
                     /translation="MRKSYMKMIIMVIIISFFSNLQANLLFDDGVPENLRNGISELYQ
                     KKKIKNSFLIEVQASGDLLIIAENGFIKSLSMSDITAFDVLNVMEEMVEIVFEKKGPD
                     KISVETEKDLKKVKKFSPDEKDQKKDEEQKYVKGGIKVFDNIEFFPWTPSSERFNVSL
                     FVTSEENWAGGAGISAGLAFLRLGVAFKKGADIVFAESDRVPWESYGANIQFDVFSAY
                     GSFVAIGFEIALYRAMEKLFEREFFIFKIGYRHKWFEAAASINVSPSVVELGLFGTKY
                     TMERYNFVISAGVLF"
     gene            complement(9036..10214)
                     /locus_tag="PL056_00045"
     CDS             complement(9036..10214)
                     /locus_tag="PL056_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45432.1"
                     /translation="MKKILLSLFIVFISFSCSFKNYSDNENLNDSDQSDHAEDSDESD
                     ESDDSDQSDDSDDSDQSDQSDQSDESDNDSGDSGNTGDSGDTGDSGDTGDTGNTGDSG
                     NTGNSGNTGDTGNTGNTGEGLFVRIIAANLTSGNGQDYDLGHGIRMLNALKPDIVLVQ
                     EMNYKSNSDANYKEFSQAILGTDYYAVESGVNIPNGIISKWPITSNGYWNDPTLTDRE
                     LMWGIIDIPGPIDIFAVSVHLHTSPSSDQVTASLVIVDEVKKHKNANPGQFYYVVGGD
                     FNGTSSVSSNGFGKDGTFYVSSPHPADDNGNVNTNANRNSQYDFVLGDNTMHGFQIPV
                     VYKSNVNYSATTYTNGLVFDTRTFSQSELDEYFPDALTSDSGASSMQHMAVVKDYFID
                     "
     gene            complement(10211..10663)
                     /locus_tag="PL056_00050"
     CDS             complement(10211..10663)
                     /locus_tag="PL056_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45433.1"
                     /translation="MTFDSAIFNHYEKLPVKDIYLYSVEIPYSQWENIRKIDDSITEA
                     NPYIEDIIEDYVHSKIKYLSIAFIFIFLAGLISLLKAAPSWKTTKKQFKISSLSTLIT
                     FIILSSLMFPSFQRSLYPFIAINLSILLAVCFIIFIIVWRLMKTSGLK"
     gene            11087..11935
                     /gene="rpsB"
                     /locus_tag="PL056_00055"
     CDS             11087..11935
                     /gene="rpsB"
                     /locus_tag="PL056_00055"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HPM45434.1"
                     /translation="MPEIRDLLKAGAHFGHQKQRWNPKMAPYIFTERNNIHIIDLQKT
                     MELIEDAKKFVRNIGMNGETIFLVGTKRQAQEVIKEEAEKAELPYVNYRWLGGMLTNF
                     TTINQSVKKLQKFDEILNSEKRRLYKKKELSDIDKKKAKLEKNLSGILRMDKMPSAIF
                     IVDPVKEHLAVHEAKILGIPVIAIIDTNGDPTKIDFVIPANDDGMRCVSIITSDIVAA
                     YMEGLDIYKQKIVTEQKDRKDKPARGETRSVAGRKVRVKRIATSENPDEQTDAHEEDV
                     TEKGEE"
     gene            11937..12857
                     /gene="tsf"
                     /locus_tag="PL056_00060"
     CDS             11937..12857
                     /gene="tsf"
                     /locus_tag="PL056_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012082906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HPM45435.1"
                     /translation="MEISASLVKDLRERSGAGMMDCKKALQEANGNMEEAMQILKDKG
                     IAKAAKKAGRVASEGTVLSIIENSNGLLFELNCETDFAAKSEGFKNLSREISGYFMKN
                     ASEEGVLVSMEDKFYDANVEKLTTDAVATIGENIKPRRFVKYSGSDSSLIHSYIHMGG
                     KIGVMLEVQSSDKDAVKKPEVVEMSDGIAMQIASMRPDCVDESSFPQDKLESEKRAFL
                     AQVMEMGKPQNIAEKIVEGRIKKFIAENTLLEQAFVMNGDIKVKDLVKETGKKVGAEL
                     KIVRFHRFELGEGIEKKENNFAEEVAAQIK"
     gene            12805..14259
                     /locus_tag="PL056_00065"
     CDS             12805..14259
                     /locus_tag="PL056_00065"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPM45436.1"
                     /translation="MKRRKIILQRKLLHRLNDLMKKVFSDIFRGASLKKTLLCFFILS
                     GYFLLSGSEAKVYTKDQFVEVFIEKADIIESLKYKIESAQADYNKVLSQYFPKIKFTF
                     GVGPHPKYEYERARIEEDGQGGYTFIESDWIKSYYDLSEYGVAIRMKGDIVLPVYTFG
                     KIKNGLDVTKAQIEAKKAEAAIGELKLRKEAAVFYWSWVMASEMFATLEPALQQVDKA
                     EEKLKEMLFEEKEGVRQKDLIKLRIEKEKLAYRHKKLVFQMETLKEVISQILGENWQL
                     ADSSMKVVEYKKEYDQIVDYMFSESPYSKYLTSGLSAYENLYELEISKILPDLGIAGY
                     FTYKYTSSVYEKNYPYPDSPYNGWDGELGIGFVFNLNFVEQGMNIKKAKSEWRAMKAQ
                     ASFAKKSAPLIIKQKYNELKSLESQVEHMKNARKFSKGWMTSEFTNYESGFNNTNDLI
                     DAVKVFFENEYLYIQSIYDYNMKVEDLIEYTGAR"
     gene            14262..14978
                     /locus_tag="PL056_00070"
     CDS             14262..14978
                     /locus_tag="PL056_00070"
                     /inference="COORDINATES: protein motif:HMM:NF017319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPM45437.1"
                     /translation="MKNSVFLLFLLFLSGVVSANETKKVENPAAAVAGNEEFNVNEAV
                     KFLESKEQILEKWANVKPKKGSAQFKQKDADMKKAVEEIIDYKFIASYIIGEKWETTP
                     EEKKEELFSKIKELFTELYLEDTFYNKSYEKKYIDKGIEKMYIKGVPQSVFVTTEVQA
                     ALKGKPVIYELIYHLHKVDGSYKVFDIELDTVSMVRNYREQFIKNVKAQSVEELIKMI
                     DKKVRNRKKEGSIFKEKKVS"
     gene            14983..15537
                     /locus_tag="PL056_00075"
     CDS             14983..15537
                     /locus_tag="PL056_00075"
                     /inference="COORDINATES: protein motif:HMM:NF014707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HPM45438.1"
                     /translation="MKEHILIHSCCAPCTTAVLEKIKSGSDPDISVFYYNPNISPETE
                     EEKRFIELSRYVEKRYGSGIEVIRGKYDNYEWNQMVDPVSTSGERGFRCKICYYIRLL
                     ETFRKATEINATTVTTTLSISPYKNYEWIDEIGMLLSERFDIKWFLEKWDYKRSIELS
                     KEYGLYRQKYCGCIFSAKERELRR"
     gene            15563..15847
                     /locus_tag="PL056_00080"
     CDS             15563..15847
                     /locus_tag="PL056_00080"
                     /inference="COORDINATES: protein motif:HMM:NF023376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HPM45439.1"
                     /translation="MGNVGKPVVEGKNIQLKLKNDDVAAGNYSNSFAITFNENEFLID
                     FIMMQPQAQMGLVTDRIVVTPRKAAELAKALSDAVKAYENMLKTRKDNIQ"
     gene            15854..16747
                     /locus_tag="PL056_00085"
     CDS             15854..16747
                     /locus_tag="PL056_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010423864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPM45440.1"
                     /translation="MSDISYFGEIAAISASLCWSITSLAFESAGKRVGSLQVNIIRLV
                     LALFFLSGMSFFANGQFFPFDATPSAWFWLTLSGVAGFLIGDLLLFQALVVIGSRISM
                     LIMTLAPVFTAILAKIILGEELAVTSFLGMMLTISGIIFVISGKKGGQERKITKYGLS
                     LAVGGAMGQALGLVLSKLGMKDYSAISSTQIRVIAGIIGFSIIFTMLKKWGVVISSIK
                     NISAMKRITTGAFFGPFMGVTFSLLAVQHTNAGIAATLMSLAPVFIIVPSRFIFKEKI
                     KLREIAGAVIAFSGVAVIFLF"
     gene            complement(16771..17394)
                     /gene="upp"
                     /locus_tag="PL056_00090"
     CDS             complement(16771..17394)
                     /gene="upp"
                     /locus_tag="PL056_00090"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HPM45441.1"
                     /translation="MFTAIDHPLISHKINWMRKHDTDKKMFRELLNEISILLMYEASR
                     DLPVREVEITTPLMKTKGFILEDNAVALIPILRAGLGMADILQELLPIAKVGHIGLYR
                     NEETLEPVEYYKKLPRDISERHVFILDPMLATGGSLSAAVDIIKREEPLSIKVLTIIS
                     APEGLAKMEEEHPDVMIYTAQVDDRLNEKGYILPGLGDAGDRLFGTL"
     gene            complement(17413..18267)
                     /locus_tag="PL056_00095"
     CDS             complement(17413..18267)
                     /locus_tag="PL056_00095"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HPM45442.1"
                     /translation="MGKIANLIKSARLAEVSLMLGFPFMGTLFAMKNFENLFSSEVFI
                     FITGLFFLCTAIYAFNAWAGYDEDGENERLTGLKDKKNRFMTATAVSGILFILTFSIS
                     GIYFVILSLVSFFLWTLYSWPKKGFKYRPVLGTVIHFLGQGIHFQMGYSVLDRTSFSS
                     ILVSIYFSLLFSAGHLNHELIDHDPDRKMNVNTGAVHFGKKIWERVSFSVFTFSTLYL
                     LTLSMFRITDIMLNWPFIFAGTVHIAYRLMFCRNDLSKERFLNERRFYRLAYFTAGIF
                     FIVMKAFI"
     gene            18392..19090
                     /gene="dapD"
                     /locus_tag="PL056_00100"
     CDS             18392..19090
                     /gene="dapD"
                     /locus_tag="PL056_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007725755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HPM45443.1"
                     /translation="MDANEIIRIISESKKRTPVRALFKGAVEEVDFSDFAEFYEGVDF
                     IEVYDELNVVREFAEKNRDVKMTRLEIDRRNSAVPLADLSKYNARIEPGAIIRDMVEI
                     GDKAVIMMGAVLNIGAIIGEGTMIDMNVVVGGRAIVGKNCHIGAGTVLAGVVEPPSAT
                     PVVVEDNVLMGANAVVLEGVRIGAGSVVAAGAVVTADVPPMTVVAGVPARIIKKVDDK
                     TRSKTELVEALRNL"
     gene            19179..21527
                     /locus_tag="PL056_00105"
     CDS             19179..21527
                     /locus_tag="PL056_00105"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HPM45444.1"
                     /translation="MKKLLVTAFFVSFFTLSGLPVEIVIPDIEFIEGDEGVIPAIEGY
                     ELDGTGEKVILPYKRVVFSGKIKSVEIVEKREITLTGPLKKGMALYRLNDMQKVEKVQ
                     FERAYLPTAAKFLFQKMPTYSGDIKEFSADIYPVIPVSDTKIIKIDRVRIDLEQSSPV
                     PMGPTKGRDTLLILTSKKVVSESKELYNFISVKRADGFKVEVATEDDYNGGDLKGIER
                     VHKIRDYLKDRYREFYFLLIIDDPLPGGKGVPMVTTRPDILEEKSYEQVPTDIFYAEI
                     SEEIDRNNNGIYGEREDKIEFSFEYIVGRIPVYNDRIDNVDKILTRTIKFIKEKPSEA
                     TYRQRFLFPTTIAYYPNQDGQMFLPKMDGGYVVKFLKENVLLDIFGSKTLVEAAGANP
                     SEFVDEEPINYANVLKQWNEGYGAVYWMGHGMPTYSVRTVWRGDINSNGYADSYELNG
                     ENFVDSDMTEKMTHDAPFVFQGSCLNGTITNNRNLAYMTLLNTAVGVVGSSQVSYGTI
                     FSDYDQSSQDLFAYGTVFIEAIADNKPPAQILQQKKQSWSSGSVLLTIKMETNYFGDP
                     SLNMNMQKCEIDEDCDDLIFCNGTEVCSKGYCEKNHDAIPCPASLDPCMPNICDEKTK
                     SCAAQASPDGFYCGTIDDPCFEGMRCNEGKCVQSGPKDCSSFDSPCSKGVCNSSNGLC
                     ELVAENEGGVCEDGLFCTINEKCIKGVCAGEDIDLPEPGSCSKVECDNGSESFTQLID
                     QSLNWTSCETDDGAKGTCYYGVCKENEDQEKTGSSGCSVIFF"
     gene            complement(21514..23160)
                     /locus_tag="PL056_00110"
     CDS             complement(21514..23160)
                     /locus_tag="PL056_00110"
                     /inference="COORDINATES: protein motif:HMM:NF017009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPM45445.1"
                     /translation="MAVKVGQYFIEKGLVTEKDVAAALEYQKKRPGYLGEILLSQELI
                     TETDLLHYLSKRFNVQYISSEKLEKIPAQHPVDVIPEKLAFEKNVYPLKYSFNNFRLT
                     LLTHEPQNIALFDELKVLLTGVNTIVPVVTTSNAIKALIYKQYKGDLSSFERLVKKGV
                     EMNILLPGSESIIGRDSVSSMGETIQGIITEDNRNRNENNATFSAILNQGEMIESTSV
                     TASMISLMPGLSRDQQGQMIEIIRVFSNLLDTSRDDSYFGHTQRVANLSQELGHEMSL
                     SDVELHDLLVASYLHDVGKKNHFTALDIKQKTDNQKLIKYAGIASKLFSNVDLSKLTL
                     SYLANMYETYNGAGVPQGLSLNEVPTGSLILLLADTYDHMTRVSKIPTATAYRQIKDL
                     LFFPDKLLLALKKIEQLDTMPQSSSSPVFEGLIISRKRFDCDDISDKLNRLNVKTYKA
                     DLFEKAAQIIKDKKEALNFILCDLDMPESTISPIKFLAALKHKKEFSEIPFYFFSQYS
                     MDRNIITAAKALKISGIFPNYHPLEMTRKVVDDIKNIKRI"
     gene            complement(23592..23852)
                     /locus_tag="PL056_00115"
     CDS             complement(23592..23852)
                     /locus_tag="PL056_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45446.1"
                     /translation="MNDTPKHILQKQLEIINKRSDAEKIERWLELTELSRTIILDQLR
                     EKHPEMSERDLIAELFKIFYRNDFTPEKLNIIAESIREYKVS"
     gene            complement(23845..24387)
                     /locus_tag="PL056_00120"
     CDS             complement(23845..24387)
                     /locus_tag="PL056_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45447.1"
                     /translation="MNPDINKFIHDVCDRLEQNSVEYMISGSIALNFYCIPRMTLDVD
                     IVIELEEEKIDAFVAIFPDSYIDKDTVRSEVRRKGMFNVIHNQTGFKIDFIIRKNSEY
                     SKLAFKNRKIKKGSGKDFRIIDLNDLIIAKIMWIQDYQSEKQMNDIRNLLDNSEKKIG
                     YIKEWCEALNLQTFGLLDNE"
     gene            24577..25062
                     /locus_tag="PL056_00125"
     CDS             24577..25062
                     /locus_tag="PL056_00125"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45448.1"
                     /translation="MVTKAFFHGRKDYRLDPKGRIPFPSLWYAPLGLENSNKVVVAKG
                     LSEEKYLEIFSVESWTEQMKIIDESPIGPARNKMLKWYVATAETVELDNQNRLRLSKN
                     LLDYADIKKDVVLAGSIETAQIWAKEVFDASLVMDDNDFDMMYNFMNDARKKIREKGE
                     R"
     gene            25059..25985
                     /gene="rsmH"
                     /locus_tag="PL056_00130"
     CDS             25059..25985
                     /gene="rsmH"
                     /locus_tag="PL056_00130"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HPM45449.1"
                     /translation="MSFVHKPVLMNEVLNLMNLNDPRSYLDLTAGGGGHAMAMINKYP
                     SMKAVLLDRDPDAVSHLKEKFAGMDNVTVAKSSFSELDKVLFLMKRDDVDIIFADLGV
                     SSHQFDTAKRGFSIQKDGPFDMRMDSESGMTALEYIKKTEESEIKNILSIYAQEREAG
                     KVARVLKKCAEEGMTTTSEFAGAIRSAKKYGKQGIDPATQVFMALRMVVNDEIGQLEK
                     MLRKGFSKLSVNGIMGIITFHSTEDRAVKNFFKDRKNNVPYYINDNDKVPYSFSPVTI
                     SGPFSAGEEEIRENPRARSAKLRLISKSSFYN"
     gene            25996..26352
                     /locus_tag="PL056_00135"
     CDS             25996..26352
                     /locus_tag="PL056_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45450.1"
                     /translation="MTGFVHVEGEKAERSGKAIFSVRKAKTKMMLFSKDVVVFIVMVA
                     VAGSLMLFNSYIKAFRNSQNVEIVKRIEMKRELILKKNEVNGAYIKMSSSAELMKKAD
                     ELQLSVVTLDKVMQIQ"
     gene            26364..28442
                     /locus_tag="PL056_00140"
     CDS             26364..28442
                     /locus_tag="PL056_00140"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HPM45451.1"
                     /translation="MPRENKLYLFFAVFISAAFILLIYRAYIIQIIRHEKLQEYSTND
                     EVVKIIKGKRGAIYDRNGEPLAVSEPKIDIAVDSKNIIEKDEMAVIISKSLKLDIKKV
                     HEILNREGHFYYLLKNAEYDSVKGLKEQKNNIRKKLKEMKMSKEINKELQDKYLNMFR
                     DFGFIIYLEGYQRTYPQGKMLANVLGFVQRNDDVGLEGIEMSYDKFLAGKEKKLRRLT
                     IPSTGESTLEIEKELDDSSSADIYLTIDSRIQFIAEEELTKMMGKTKANWGGVVVMEP
                     ATGKILAMANYPTYEPDRYFKYSPKERRNYTIANLFEPGSTFKVFSILAVLNENLAKP
                     GEMIFGENGRFSFGRRWVRDSHPYEWMSIRDIVINSSNIGTIKLADRLSREQLYDYFT
                     DFGFGTKTKINLPGESFKPIRPAAKWYPIDKGNISFGQGISVNMVQMARAYASVYNGG
                     VLWQPVIVDRIVNPSNEKILFQTVPEPKRIAFKFSSDKRMVSMLEGVVEEGTAKSAAL
                     EGIKVGGKTGTSQIFDFTAKKYSWDKVVCSFIGAVPSNDPAFVIMVVIEQPEGREFGG
                     TVAAPVFRNIAARSLPLYGIYVDRKAKDKSIIPDMPGKDITGVAVEASSEKESEESGA
                     MDFIKIPSFINSDSTKALQTANKTGLDVLFSGDPSRKIVNQYPAPGEFVPFGTVIMLE
                     TEEPQINENN"
     gene            28429..29907
                     /locus_tag="PL056_00145"
     CDS             28429..29907
                     /locus_tag="PL056_00145"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HPM45452.1"
                     /translation="MRTTDFIKLISSHIEKEMSTADERELLRITADSREVDEKTVFFC
                     IKGAKADGHDFAEQVIKKNAGLVVGEKELKMERYVRVNSVKEVLKLISPAFYNYPDLR
                     MKMAAVTGTNGKTSTTFMIESMLAGIFRCGVIGTLGYRYGGSTFDAPNTTPINWKWYS
                     LLNEMEKAKTEVVISEVSSHAIEEERIEKTLFDVAVFTNLTRDHLDFHGDIENYFQAK
                     KKLFTKHLKKGAVSVINYDDIFGKRLYEEICDSVKCIRISESCHDAELKIFIKKLDSS
                     GSTVDISFEGLCKTVKIPLLGKYNIYNTACAFAVCSVLAGREMAFESIGSRVSVPGRL
                     EQIGEESIFVDYAHSPDALENILKTVTDLKIFKRVITVIGAGGDRDRGKRPLMGEVAT
                     RYSDIVILTDDNPRSEDPENIIREILAGCNPSESVIEIINDRSEAIRRAVFIKTNEDA
                     LIVAGKGAENYQITHEGKRFFSDREEIEKYLKELHNVHNSTD"
     gene            29885..31180
                     /gene="murF"
                     /locus_tag="PL056_00150"
     CDS             29885..31180
                     /gene="murF"
                     /locus_tag="PL056_00150"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HPM45453.1"
                     /translation="MFTTALIEKVLRASGIRKEFAPGIISNVSIDSRSCRKNSFFFAF
                     KGENVDGHDFIPALLEKGVLCAGEKDLPENDLYIKVPNVLDFLSALAKERRKLFKGTV
                     FAITGSSGKTSTRQLTVSMLQLLSKSVHATSGNLNNHIGLPLVILNTPMNIDALVLEM
                     GMNHAGEIRHLTEIADPDLSVITNIGTAHIGNFRSQKDLAFAKLEIFEYSKGIVVADT
                     GNEFIKKWVGSNRSKRKIVEYSSSDSEKIAPEFDYLEKYMVENLYTASVTVKASEGII
                     PDLGKVVTNASFPSMRGEIRKVENRTFVVDCYNANPGSMRSSIENFYEKYSRKSGSET
                     YLILGSMFELGEFSKKMHEELVNYLKNLNLLKRVFLIGCEFEKTRPGFLNEKKVLFLG
                     DITDIPEHLPKEGVFLLKGSRGNRMEKIFDLLEKIGGVD"
     gene            31177..32469
                     /gene="murD"
                     /locus_tag="PL056_00155"
     CDS             31177..32469
                     /gene="murD"
                     /locus_tag="PL056_00155"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HPM45454.1"
                     /translation="MKALVIGLGVSGTSSAKFLKKFGNEVSIYDDNRGVLDKFSGTYP
                     VYNRDESYDTVIISPGVPSDHPVVMELRAKGTEVIGEMELAGRYLNNEKVIAVTGTNG
                     KSTTVSVACAMLQEAGFKAFLCGNIGEPVIEGVNRDYDFLVIEISSFQLETLKSIRPD
                     VSMILNVTPDHLDRYSSYEHYLLTKVELAKLTKPDGLVILNGGDEALVAACRPVNVAK
                     KYFSVKGTGDISYRNGSIYFGNSELKIDDLYLKGLHNVENIMACVLGVTPYIDDVNVL
                     RRCLKNFKPLAHRTEFVDEFEGVVFIDDSKGTNVGAVEMSLAGFEDGKVVLILGGVDK
                     GGSYEPLRILAEKKCRGVVLIGEAKDKIKSYFEGFRDLEQAESMKEAVEKSFMLAKND
                     GVVLLSPACSSYDWYKNYKERGNDFRNRVAELKRGLEK"
     gene            32466..33551
                     /locus_tag="PL056_00160"
     CDS             32466..33551
                     /locus_tag="PL056_00160"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HPM45455.1"
                     /translation="MTRALKLYFSIFFLLVVFGMVMVFNIRLFNVPSIQNALLPLLKG
                     LAMNLLIASLGFTASYLVNLEAIRSWIKYLLIFTILILISTYIIGTEINGSKRWIDLH
                     FMMIQPAEFAKITVVLYLSEVICNKRDRIGILKELVYPFAVVLIVVSLIAFEDLGTGA
                     IIFTVAIFILLMAGMRVKYFAVLIVLALAGAAFLVFMKPYRLDRIKASFDPWQYRKNI
                     GYQQVQGEVSLGRGGVAGVGFGNSQKKLRYLPEAHTDFIFAIIGEEFGFIGVTILIIL
                     FLLMFITGSYFALTVHNRFGFYLISGFVVMLALQTLINLGVVTSCLPNKGVPLPFISY
                     GGSALLAYSMMVGFILNAVTADNRYSS"
     gene            33563..34657
                     /locus_tag="PL056_00165"
     CDS             33563..34657
                     /locus_tag="PL056_00165"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HPM45456.1"
                     /translation="MEKIGTRFIILVAGVNKKERLKPALAVADILRKWGIMSMFAFPD
                     GSPFIKTVTEKGYETVNVDKFPEKKGFVGNFLRSLKRKSSLKNSMKTLSTKHIQAVLT
                     LGGITSVPIMDAAVKMNLKIFMLEPNAVMSDCNKEFISCAHRIYLPFAEMIDSVPRSK
                     ALVAGVPVEKDVMTAEKRNIPTNKKILVIFTCRKDSYSINELVRTMFRKYPEMRKEFF
                     ILQETGEKDVASIQRFYDEVQVEALCYMQYENRGKYYKTADVVISRPTADVVSELLAM
                     HKPGIFLPLSEHYDVYQKHNAKVVAKKEIGFIVEDGGSMPMRVKKLYTALNSFLVGNA
                     KIKQNIDKLDYEKAAMKVAEDIEKNLSGKV"
     gene            34629..36017
                     /locus_tag="PL056_00170"
     CDS             34629..36017
                     /locus_tag="PL056_00170"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HPM45457.1"
                     /translation="MKRIYRGRCNGMHVHFVGIGGSGVSGLALMAQRMGYEVSGCDEN
                     VSPYFKMVESKGIKCYSGHSSDHMQGVDLVVRSSAVPMDSEEIRYALKKGIVVISRGT
                     FLSKMMEGKDVIGVAGSHGKTSTTWMIYHILKKAGLNPSVYAGGKSGGVSNISEGSPY
                     IIELDESDGSMFETTPGILLINNLEFEHADFYSNPEEMLNSFERYLLSWEPDTLIIGR
                     GYDLSDALFSMFTPSSFLTVAELKENGSFQNVSGLDFFVKEEAVYLLSDDTEIYVGSV
                     REPAHILQNRSAALLASLSFLKKINVTIPKIDFKKLWTEIPVVDRRFQVVGKYKGVDL
                     IDDYAHHPSEIRALVEQAELKYGNFCLIFQPHRVSRFTVFYDKFLEVLKYVEPLIVLP
                     VYNAGETVKGADTESFYRELKQNGSEVYYFKSVDDAGVFIKKNIEGLHVSAVIAAGAG
                     DLNKVFELVGVK"
     gene            36014..36856
                     /locus_tag="PL056_00175"
     CDS             36014..36856
                     /locus_tag="PL056_00175"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HPM45458.1"
                     /translation="MKLKKNALFGNLLSIRVKDAVCDLYFPESIDDIVRFTHEFNDFY
                     VLSGGSNIIAGHVMKPVLYMGGMIGTSDTEDVSEHAVKTYMPAGVPISMLINYSMKNG
                     LSGVEFMAGIPGTLGGAVMGNATPADYSWDDVAGSVYYVKNGAVSVLVPQFSYRSLDN
                     PPEGNFVMLGVDLVLIKDSVENVRKRVMYHLSRRIKIKGRSAGSLFKNPEGEKAGALL
                     DKAGMKGFYVNDAGLSEDHANIVVNKGNGVFNDFCVLKDTAIAKVNEMFGITLCPEVK
                     FWYE"
     gene            36849..37754
                     /locus_tag="PL056_00180"
     CDS             36849..37754
                     /locus_tag="PL056_00180"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HPM45459.1"
                     /translation="MSKLNKNTKIAVIAGGMSDERSVSIETGGMIHKTLTEGGYVNTV
                     FIDAGFDLDLKLREFSPEVVVNGLHGKYGEDGTVQGLLEMMKIPYTNSGVSASAAGMD
                     KIICRAVMKELGIRVAEGIVFTAENDLMPPFDLPVVIKDPVNGSSRGVFIIKDMESWE
                     KTVSELVKGKKYLCERFFKGREINVAVLMNEVLGDVEIIPANEFYDFESKYMSDLTKY
                     IVDPDYNEKVSRDIWRASQLLHTALGCKGVTRSDFIVSGDDYVMLEINTLPGMTSHSL
                     VPMIAKKRGLSYLNLIELLINEALG"
     gene            37754..38614
                     /locus_tag="PL056_00185"
     CDS             37754..38614
                     /locus_tag="PL056_00185"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HPM45460.1"
                     /translation="MEKFKKIASTLFYSFVLYFLTFIFVAFFTLSAQVLREWKQSEMV
                     KVSDDRVEISGNNMLSRKEVLLISGLENEKSWFDLDERRMEAYLLSSGWVKRAFVTKL
                     FPDSVNIVIEEFVPAIVVNSRRSSLSPNEKKDVSTLWFADSEGIVFKRTFPGETTAAL
                     PFFHIDNDAVSEKLRGEKIVTAVMISKAWNDQRSMCSIKSIKFDMSSGFSADCEAPDS
                     MTTYLAFGNEFSNNEISTMKEMFSNISGKLSSENKWAGEYIFEKTEKDKKIRVIVGKV
                     FKNVNRGSNA"
     gene            38607..39836
                     /gene="ftsA"
                     /locus_tag="PL056_00190"
     CDS             38607..39836
                     /gene="ftsA"
                     /locus_tag="PL056_00190"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HPM45461.1"
                     /translation="MPKNDSVIVALDVGTKKVTTLVAKVENDKKVQFIGKGVAKNENG
                     IIAGSVVNMEATTRAIHESVTEAEKIADIHIKSVYIGLSGEHVSSMNSHGVVTVRGRE
                     VAQTDIDRVIEQARNVSLPSDRQIINVEIGEFVVDDQAGIRNPKGMAGRRLEVKVHIL
                     TSSTAMLRNLAKSVEDAGLHVEDVLVNGIAGSWAVLEEDEKQLGVALIDIGDGTTDIT
                     VYHKGDPVYTTVIPMGGRYVTTDISYAMRIPPVEAERIKCRYGSARPDYVSSDEEIEV
                     STIGTEEKKVKKVHFLASVLAPRVEEILMAARKRLNEEMKESLIQTNIVLTGGTAELK
                     DIQFLAKDVFEQPVRIGKPKINEASSGFKDVLSSPSFSTAVGIINYFAERGKMKFRDK
                     NGGFFSKLGDFFRRFFE"
     gene            39848..41221
                     /gene="ftsZ"
                     /locus_tag="PL056_00195"
     CDS             39848..41221
                     /gene="ftsZ"
                     /locus_tag="PL056_00195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HPM45462.1"
                     /translation="MTGILNSMVVKIGVVGVGGGGCNAVDMMCDERDRINSGADGRNY
                     SVFNNVLIIAANTDVQALEGNKSAEKLQLGPNLTKGQGAGGVPEIGKKAAEESKVDIA
                     TVIDGLDMLFITAGMGGGTGTGAAAVVAEVAKNSGILTVGIVTKPFDFEAKRKQRYAM
                     EGMDALRKQVDTLVVIPNQRLLDIAEDSDMAIDMFKKPNEVLIYAVRNLSELITNKSF
                     INVDFADVRQTMTNMGLAMFGFGSATGENAAINAVKEALNNPLLADFTISGSKKMLVH
                     FGGAPTPMKEFASAAKYLTDQLHEDGDVIWGASVDEKADKVYALIVAEATEDTISTID
                     LPKSMKKVEASDQGKLFEVNEKPVEVLEQRSDFVEMDEIEVDVLAPAAQNTGSRDNDE
                     EVFNIDREKISATNVFDDEIIVTESKYNPEDTTIPAYLRYLNRNKQNEQVVTEKKLDR
                     IQSSKNK"
     gene            41225..42547
                     /locus_tag="PL056_00200"
     CDS             41225..42547
                     /locus_tag="PL056_00200"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPM45463.1"
                     /translation="MTEKPLLVYPDRTDIAVSNLAVHWFFNAFNEFEPDLLTSDSKVG
                     IFHKRDINKTPFVFVSLSYGPDIIKFAKILRNSGIPLRKEDREKGMFPLFICGGIAAL
                     LNPEPLTLIFDAVFLGEGECMKDDIVKMFSMETREGVLDHINALEYAMTDVKPDARQV
                     IAAGGSFFVSSNEILHKLGNCFGNRKIVEMNRGCTSRCRFCAASYAYRTFREPDGKKL
                     MDFVKKTAERKEGLALMGTSLADVSSFDEILETCAASGCTVSLSSLKITEINEKRALL
                     LKKCGVRTVTVAVESADPDTRQKILKNITDTDIFNAMEILKKHNLKAKIYLIAGFPET
                     DLQKEAESVIELLKELDKRNLLHDVTISVAPLSPKPLTPLQDAKFMDKKQYKNYMVAL
                     KKGCSVFGKRIKLDFFSYRESEIDYKTGFLKGADLIDLINENIEKGDV"
     gene            42547..43536
                     /locus_tag="PL056_00205"
     CDS             42547..43536
                     /locus_tag="PL056_00205"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HPM45464.1"
                     /translation="MAEKIIVFCENDRLARYCKVTFELLSVARSLAENRGFSVIAVSF
                     KGHSEGSDALFASGADEVIEPHWTEKEGLFNLETVTHIASEVIRKLDPVIVLFGATDL
                     GRVLAPKVAANVRCGITADCTGFEINKDGKLVQIRPALGGNILAHIISPERLPQMATV
                     RPGVFKVKEFFNHERPIEKMFFEIPNTRRHVIENSRVSNMSAECEKIEDAKIIVAVGH
                     GVRSKELLKKIEDFAKSIGGVCACSRKIVESGWMEHSRQVGQSGKTISPDIYIAIGIS
                     GAVQHLAGMKTSMKIIAVNTDANAEIFSVAHTGFVMDASIWLEKATEFFTKKV"
     gene            43538..45034
                     /locus_tag="PL056_00210"
     CDS             43538..45034
                     /locus_tag="PL056_00210"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HPM45465.1"
                     /translation="MDRLKNIFSRDIEPSDRYLADESRMQGGDASKVLFPENSVEVAE
                     ILKYSSLNKIPVTIYAQGTGITGASVPFGGIVISLERMNKIGEIELDAESGEHFVKAA
                     ACATLNGIKDILNGTELFYPVDPTEMSASIGGTVATNASGALSFRYGPTRKWIRSIEI
                     VLMDGSIHKIVRGQNFADDSGSFNVFGKSFRIPEIKMPKCKNAAGLFSENAMDIIDLF
                     IGSEGILGVITEVEIGLAPKHASISVVKFFDTEIEAIDFTAYLKDSKDVMPDFIEYFN
                     GSGLEMIREKAKKDPQSVSMPKISDNANAALFFDIEIKDNNINAAFNKLLSLNVDWKG
                     SWCAWENIEKERIKNFRHALPETVNEYIAGVKKKHPSIHKLGTDFAVSDEKFGELVEF
                     YDSVLNRSGLKYVSFGHIGDNHLHINFLPSNYDELEKGKEIYKVIAEKVIELNGSVSA
                     EHGIGKLKHRYLEMMYGAEGIREMRRIKSVFDPHFLLNRGNMFPCGEL"
     gene            45034..>45133
                     /locus_tag="PL056_00215"
     CDS             45034..>45133
                     /locus_tag="PL056_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta"
                     /protein_id="HPM45466.1"
                     /translation="MNIAVLIKQVPRTDKIKIDRETGTLIRKGVESI"
BASE COUNT        14290 a         7905 c        10616 g        12322 t
ORIGIN      
        1 gcatcaacga tctccatccc ttcggtgact gttccgaaag cggcatagtt gttgtcaaga
       61 gaagggcagt cctctgtaca gatgaagaac tggcttgtag cactgttggg ctcactggta
      121 cgagccattg aaagggtgta tttcttgtga aggatgttgg gattcctttc gagttttatc
      181 ggttcgacca ttgcggtttt ctcgtacatt cctttaagca ttcctccgcc ctgaatgaca
      241 aatccatcaa taattctgtg aaatatcagc ttgttataga attttgcatc gatatgcctg
      301 atgaaattct ttacagtaat gggcatatct tcgccataaa gacccacagt gatcttcccc
      361 atggttgttt caaaaaccgc tttgtcagta attttcattt tctcctccta ttaaacgaat
      421 aaaacgagag catattataa aagaaaaatt atttttcaaa ttatatgtta tagaattttc
      481 ttgttagctt tacggtatta tggtatttat acataatttt tagtttctgt tcccggagga
      541 gatttaaatg tcagaacatt ttgcggtttc aaaattaaca aaacatgttt tttgtgtttt
      601 gtcggtaatt ctgttgacgg ttcaatcgct ttacgggctg aatccggaac agaagataaa
      661 caggtttaca acagagtgtt ttgattcatc aaaaggtctt cctcagaaca ccgttgctga
      721 catcattcag agcagatcgg gatatattat tctggcaacg caggaaggac tggtcaaata
      781 tgacggcaga aagttcacca tgatgagcga gaacggtcat ctgaattctt caagcaggaa
      841 tattaccgga atatttgagc ataaaaacgg aaacacagtt ttttcaacgg ccgccggaat
      901 tattgatatt aaagaaaaga aaaccctttt tgacggtgtt ttcataaaag attttgttta
      961 tgaccgcagt ggtgttctct gggcggggac atacagttcc ggtataataa gagttgcaac
     1021 tgacggatct gaaaccgttt ttacgaaaga aggcggaaag ataaattcca atgttgtcaa
     1081 ttcgcttttc atgagttcaa aagggaagat atatgccgca accctggagg gtgtctcccg
     1141 tcttgaagaa gataaatttg tccctgttga cggaatattc gcattcggat acagaatagt
     1201 cgaggacaaa aacggctgga tatgggtggc tacagaaaga ggacttgctc ttataaaaga
     1261 tgataagctc gattatattt atactgcaga agacggcctt aaggatgaat ctctcagatc
     1321 tgtatttgcc gacagtcttg gaagtttgtg gataggaacg gaaaaaggtg atctgatcag
     1381 atttttcaag gggaagtttt ccaagctttc aaaatcaaac acgaagacag gagccattat
     1441 ttcgatattt gaagacaggg aaggcaatat ctggttcggt actgaggcga ccggagcgtg
     1501 cattgtgcgt gacggaacgg tttttcaggc aggtattaac acaggcaaca tccgctcact
     1561 cactgaaacg gcagatggag aaatatgggc cgcaacattc ggagagggaa tatatgttct
     1621 caaaaaagac gaaactgtaa caaactttac catgagcgac ggacttagaa gcgattctat
     1681 cgccacaata tttgccgaca gtcagtcgag aatatgggtc ggtaccagaa ggaacggggt
     1741 tcagatcttc cagaaaggca gattctatga tattgatgct ttcggaaaga acttttctga
     1801 agcccaccct gtatcgccca cacttttctt tgaagattcg agaggtaata tctggatagc
     1861 tgacaggcac tcacataagc cggtatttac atggaacaac ggacagatga cctcttatga
     1921 gatcattaaa ggggaattat ccatacttga tatagctgaa agcagaaaag gtattgtgca
     1981 ccttctttct ctcaaggagg gtgtttttga atttgatgcc aacagcggcg aattcaggaa
     2041 tattcctgta aagccggatg cgaaaatgac ggcactgttc tttgacagac aggacaggat
     2101 gtggataaca accctttctc aaggcatcat tataaaaacg gaaaagggga tagtggaact
     2161 gaatgaagcc aatggactct acagcaatac cgttcatgat attgttcagg atggcagtgg
     2221 ggcttactgg ttctctacaa acagaggaat tttcactata tcggagaagg atgccgaaaa
     2281 atttatcagc ggagttgccg acagacttag ccaccgtctt ttcaaggaag aggacggaat
     2341 gccggcaggt gaatgtaacg gcggctctca gccttcggtc ataaagtctt cagacgggac
     2401 gctctggttc cctaccatca gaggtgtcgt cacgatagat ccttcaaaga tagagcagag
     2461 agtcgagctg ccaccggtta cgatcgtcag tgcgattatt gataatgtca accatatcga
     2521 tcttgcagcc agaagcgagc ttgtggtgcc ccccggaacg aagaatattc tgataaattt
     2581 tacatcgctt tatttttccc atcccggcaa agtgcggttt gaatacatgc tggaagggtt
     2641 tgacggcagt tggaacaatg cgtccgcaaa caggatggta aattatacga atatcgctcc
     2701 tggagtttat aaatttctcg taaagtcata tcttgcagac acacctgaaa gttattctga
     2761 gacctcaatg cgtctggaga tcaagccttt cttttatcag gacagcagat tccggtcaat
     2821 agccgcaata attttccttc ttatgatcct tgcagcgtac aacctgaaga taaggataca
     2881 caagatgcgg gaaaaagagc ttcagaagac agtcgatgaa agaacgcagg aacttttgaa
     2941 agtgaatgaa aaactgagac agtctatatt aaaagatccg atgacaggtc tttgcaacag
     3001 aagatatctg tttgaaatag aacagcccag atatgaaaga atgctcttcg catcgaaaaa
     3061 gaatcttcag aattctgacg gaaaacctgt tgaaccggaa aaagtgacag gtttgtttct
     3121 catcgatata gtcggtttga aaaagatcaa tgaaaagaga ggatacgatt tcggagacag
     3181 acttctgaaa gcatttgcag aatcactgaa aagcagtgtc agaaaagatg accttgtcgt
     3241 cagatggggc ggagatgaat tccttgtaat actcaacgcc actgatcaga gccatcttcc
     3301 ggtatatgca aaaaagatac tgtcgatggg actggaaggg ataaaaatag aaggcgaaga
     3361 tccgatgaag atagttgtca cgaccggttt ttccgcaatg cctttctatc ctggagaaca
     3421 cacgcttaac tttgaagaaa gtctgctgat gtcagatatg gctcttttca agtcgctttc
     3481 aaaaggtccc ggttctataa aacaggcagt tccaggaagt tttgtacctg aaaaggagca
     3541 gatagattct tttatgaaag acattgatga agggataaaa gacggatttt tcaacataat
     3601 tgatatttga catttattac ggagattcag atgaaggtta gtcagatttt cttccagaca
     3661 ttgaaagagg tcccgaaaga ggcgcagata gaaagccacc gtcttttgta ccgggccggg
     3721 atgatccaga aactcgcaag tggaatttac agttaccttc cgttggtttt aagaagtgta
     3781 aggaaacttg agaacataat tagagaggaa cttaacaaaa gagggtgtca ggaagttctg
     3841 atgcctgccg ttcagcccgc ggaactttgg gaagagagcg gaagatggac ttattacgga
     3901 cctgaacttc tgagattcaa ggacaggaaa ggcggagatt tctgtctggg cccgacacac
     3961 gaagaggtta taaccgacat ggtgaggaaa aatctccgca gctacaaaca gcttccgtat
     4021 aatctttatc agttccagag caaattcaga gatgaaataa ggccgagatt cggacttatg
     4081 aggggtcgtg aatttatcat gaaagatggg tattcatttg atattaatga agaaaatgca
     4141 aagaagtcat atcagttgat gtttgaggca tacaatgcca ttttcaacag atgcggattg
     4201 aaattcaagc cggttgatgc cgccacaggt gcaataggcg gagatatgag ccatgagttc
     4261 caggttcttg ccgaagcagg agaggatctg atcttgagtt gtgacaaatg cggttatgcc
     4321 gcaaacctcg aaaaggcgag gtcacagttt gtcccgtcag cagaagatgt tgaagaactt
     4381 ccgctcaaag aagttcacac cccttcaagg aaagctgttg aagatgtcgc tgagtttctt
     4441 ggcagaagtt caaaagactt cataaaatca atgatataca tcgttgatga tgagccggtt
     4501 cttataatgg ttccaggcga cagagaggtc aatgaagcaa aaatacaggc gtttttcagg
     4561 gcaaacagtg ttgctcttgc aaatgatgcc gttattgctg aagttacagg tgccgaaacc
     4621 ggctttgccg gtcctgtagg gcttaagaaa aagataaaga cggctgttga cagacttctt
     4681 acgggaagaa aaaatcttgt cgccggagca aataaaacag accatcatat tgaaaacatt
     4741 aacataaacc gtgattttac gactgatgtc acaggggact ttgttgttgt cagaaaaggt
     4801 gaaaaatgtc ctgattgtga tggaacactt gaagaataca gaggaataga agtagggcag
     4861 gttttttatc ttggaacaaa atacagtgaa aagatgaact gttcgtttgt tgatgagtcc
     4921 gggaaaacgg ttacagcggt catgggctgt tacggactcg gtgtgggcag gactgttcag
     4981 gcggcaattg aacagaacca cgatgaattc ggaatacagt ggccgatggc gcttgctcct
     5041 tatgaagctg ttgtacttcc tcttcagatg aataaaccgg aagttgtcga ggcggcggag
     5101 aatatttata aaaaactcct tgaagccgga gttgagaccg tgatcgatga cagagacgaa
     5161 agagccggat tcaaattcaa tgatgctgat cttgtcggat atccgcttca gataattttc
     5221 ggcggcaaat cacttgaaaa aggtgtcgta gaagtgaaga tcagaaaaac aaacacgaaa
     5281 cttgaagtaa aacttgaaga aataattgat tttacagtga gttacaaaaa gaaggaactt
     5341 ggactgaatg attaatatcg taaaatctgc aattacagca gttattcttt cagtgatctt
     5401 tttttccata tctcttcatg ctgaaacggt tgatttccct gaaaagatac agatagatga
     5461 acagagatct gtcccattca gaaattatgc ggtttttgca ccggtttcct tttctttata
     5521 tgccatcggt ctttatgttg ccggaacaga aacagataat atgaagctca agaattctga
     5581 tgagccgatg gcaatacagc tggtctcact ttaccgtaga ttgaaaatga agacgctgat
     5641 agacgagctc agaaggggtt tcagttacgg catggatcac gataaaaagt ttttgaaaac
     5701 cattcaggaa agaattgatg attttattaa actgcttgaa aatacaggta aaaatcccag
     5761 aaaatacaac gccataacat ttctttatgt gccgaaggaa ggtactcatg tgagtttcag
     5821 gagtgagtat cttggaacaa ttcccggaca tgattttaaa aaagcgcttt ttggaatatg
     5881 gcttaataat aattgtgcca atgatgaatt aagagacaga gtgataaaaa ttagaaaaaa
     5941 gtagtgaatc cggaggatag ttttgaacag caatataccg acactttctt caaatacaat
     6001 atcaagttac atacacagcg ttctttcagt tccggatatt tcaagggaag atgaagtgga
     6061 acttttcaag aaattcaggg agaacggaga taaagaagcg gcacagatac tagttctgag
     6121 caatctgaaa ctggttgtca atcttgcata caaattcaga aagttcagag atgtaaccga
     6181 ccttattcag gaaggaaatc tgggacttat gacagcaatg cagaaattcg atatcgagaa
     6241 aggggtccgg ttttccactt atgccacatg gtggatcaaa gccaagatac aggaattcat
     6301 aatcagccag atgagtattg tccgatttgg caaaagcagg gatgagagaa aacttttctt
     6361 taacatgatg tcgaccataa aggatattca aagttatgac agtgacggag agataacaaa
     6421 agaagacttg atccttgaag ttgccaaacg gcttgatgtc ggcccggaaa aggttattga
     6481 agcgttgcag gtggtctctt catacaacga tatttcgata gatcagacca tagaagggac
     6541 ggaaaccaaa atgatagaac ttagggataa gaccgatttt gatgaagaga tggatgaaga
     6601 aacccgcaac atcagacttc agaatgcgat cgtgatgctc aacgacagag aaaagttcat
     6661 tatctggaac agatatatgt cagatgagcc gatgaccctt gaagagattg gggaaaagta
     6721 tgaaatatct aaagagaggg ttcgccagat agaggcgcgg gcactggaaa aaataaaact
     6781 atttacttca gacagtcata ttattgaaca aaccggcaca gtttaaaaga ggagaggata
     6841 tgatagaaca gattaaaaaa agagcacatt tccttgaaaa tgagcttaaa aactgtggta
     6901 ttctgaggat gcctgatttt gattccaatt acagttgcgg cgatatagat attaatttag
     6961 cggtaaatgc gattgatcac acagtacttt ctttcaatgc gacagaaaaa gatattgata
     7021 aagcatgtgc cgatgccgga aagttcaagt tcaaggcgat atgcataaac cctgtgtggg
     7081 ttaagagagc atttgatcgc agaaaaaagg aagggcatga atttctgata gccacagtga
     7141 ttgatttccc gctcggtgcc tcttcgctta atgcaagaat agctgaaaca aaacaggcgg
     7201 ttgcagatgg agccgacgaa atagatatcg tcataaacat agggctttta aaaagcggaa
     7261 tattgaaaga gacatataca aatctcaaag aggtcatcgc atctgtaaaa agttattaca
     7321 aagttattct tgaagcttct gaactaactt ctgaagaaaa gattgacggt gcccttatca
     7381 gtgtttttgc aggagccgat atgctcaaaa cttcaacagg tgtcaacggc aaagcatcgg
     7441 tcgaagatgt cagaattctg agaatgatcg caggatcgca cctcggtgtc aaggccgcag
     7501 gcggaataag agacaagcag acactcgttg aaatgatgaa agccggtgcc gacaggatcg
     7561 ggtgcagcag ttccgtaaat atcatcaaca gttggtagaa acatgtttcc gatcatcatg
     7621 aaacggcata ttccgaactt tcttaattat gccgttatac cggttatttt gctgtttttt
     7681 atcgcatgta gcaagattga aaaagacgag gtcggtatct ttaaagaatt catggagtta
     7741 aggaaagctg gcaactacgg tgcggcatat gaaatgcttt ctgatcctac aagacaggtc
     7801 ttttctaaag aggcatttga caactattgc tttgtatacc gtgtcataga ttttgaagtt
     7861 tttaaagaga gtcggggcta tgtccgttct gtttataatt tttatgacaa gaaatacaaa
     7921 aaggattccg atgaacttta cactttctac atttcttcaa acaccgaaac agtcaggatc
     7981 aaagagggaa agatactttt tccccacatc ggatttattg agatgagaaa aaacattgaa
     8041 gaaagaaaaa cggatgaatt gaaagagacg ctaagaaaaa tgataattat agatccggaa
     8101 aatccggaag tgaatcaaac tgccaaaaag atggaacttc tgtccggaag tgaagaatga
     8161 gaaaaagtta tatgaaaatg atcatcatgg tcattatcat atcttttttt tcaaatcttc
     8221 aagcgaatct tctttttgat gacggtgttc ctgagaattt gaggaacggg atctctgagc
     8281 tttaccagaa gaaaaagata aagaacagtt tccttattga agttcaggcc agcggagatc
     8341 ttcttataat tgctgagaac gggtttatta aatcactctc aatgtctgat attacagctt
     8401 ttgatgtcct taatgtaatg gaggagatgg tagaaatagt ttttgagaaa aaaggtcctg
     8461 ataaaatcag tgttgaaacc gaaaaagatt tgaaaaaggt taaaaaattc tctcctgatg
     8521 aaaaggatca aaaaaaagac gaagaacaga aatatgtaaa aggtgggata aaagtatttg
     8581 ataatattga gttttttcca tggactccaa gttctgagag gttcaatgtc tctctttttg
     8641 taacatccga agaaaactgg gcgggaggtg caggaataag tgcaggactg gcgtttttaa
     8701 gacttggtgt cgcatttaag aaaggtgctg acatcgtttt tgccgaaagc gacagagttc
     8761 cctgggaatc ttacggtgca aacatccagt ttgatgtttt ctcagcatac ggcagttttg
     8821 tagcgatcgg ttttgaaatt gcgctctaca gggcaatgga aaaacttttt gaaagagagt
     8881 tctttatttt caagatcggt tacagacaca agtggtttga agcggctgcg tccatcaatg
     8941 tttcgccaag tgtagttgaa ctcggtcttt ttggaactaa atatactatg gagaggtata
     9001 attttgttat atctgccggg gttcttttct gatcatcagt cgatgaaata atcttttaca
     9061 acggccatat gctgcatgct tgaagcacct gaatcacttg tcagggcatc agggaaatac
     9121 tcgtcaagtt ccgattgaga aaaggttctt gtgtcgaaaa caagtccatt tgtgtatgtt
     9181 gttgccgagt aattgacatt tgatttgtaa acgacaggga tttgaaatcc atgcattgtg
     9241 ttgtcgccga gaacaaaatc gtattgactg tttctgtttg cgttcgtatt cacatttccg
     9301 ttgtcatcag cggggtgggg tgaagaaaca taaaatgttc cgtcttttcc gaatccgttt
     9361 gaagatactg atgaagtgcc gttgaaatca ccgccgacaa cataataaaa ctgtcccgga
     9421 tttgcatttt tgtgcttttt aacttcatca acaataacaa gggatgccgt tacctgatca
     9481 cttgaggggc ttgtgtgaag gtggacactc actgcaaaaa tgtcaatagg ccccggaatg
     9541 tcaattatcc cccacataag ttctctgtct gtaagtgtcg gatcattcca gtaaccattt
     9601 gaagttatag gccatttact tataataccg ttgggtatat ttacaccgct ttcaacagca
     9661 taatagtcag ttcccaagat tgcctgagaa aattctttat aatttgcatc ggagttgctt
     9721 ttataattca tctcctgaac aaggacaata tcgggcttga gtgcatttaa catccttatt
     9781 ccatgcccca gatcgtaatc ctgaccattc ccacttgtaa ggtttgccgc aattatcctg
     9841 acaaacagac cttcaccggt attgccggtg ttgccggtgt ctccagtgtt gccgctgttt
     9901 ccggtgttgc cgctgtctcc agtgttgccg gtgtctccag tgtcaccgct gtctccggtg
     9961 tcaccgctgt ctccagtgtt gccgctgtct ccagagtcat tgtcggactc gtcggactgg
    10021 tcggactggt cggactggtc ggaatcgtcg gaatcgtcgg actggtcgga atcgtcggac
    10081 tcgtcggact cgtcggaatc ttcagcatgg tcggactggt cggaatcatt caaattctca
    10141 ttgtcagaat agtttttaaa actgcatgag aaggaaataa aaacaataaa taatgaaagt
    10201 aagatctttt tcattttaac cccgaagttt tcattagtcg ccagacaata ataaaaatta
    10261 tgaagcatac tgctaaaagg atagataagt ttattgcaat gaaaggatag agacttcttt
    10321 gaaatgaagg gaacattaat gaggaaagta taatgaatgt aatgagtgtc gaaagggagg
    10381 atatcttaaa ctgttttttt gtggttttcc atgaaggggc ggcctttaaa agagagatca
    10441 gcccggcaag aaatataaaa ataaatgcaa tggagagata cttgatttta gaatgaacat
    10501 aatcttcaat gatatcctca atatatggat ttgcctcagt gattgaatca tcgattttcc
    10561 tgatgttctc ccactgtgaa tagggtattt ccacagaata caggtaaata tctttaacag
    10621 gaagtttttc atagtgattg aaaatggctg agtcaaaagt cattttttca acctcaattg
    10681 atttggagca tatttccatt gaatcgaaaa tcctgtctgt tgcttccttt tctgttgtta
    10741 taaccgttac aggaaaacat ataatcctgc tttccagata agaaaaaccg tataaaagaa
    10801 aataaaaaat ggtaaaaaaa acaatcaggg tcagtatgtt tcctgctatg gatccaaagt
    10861 agcccgtttc tttcaaatct atcacccgaa aaagaaaaac tcgattgatt atacagaaaa
    10921 aacggatcaa gtaaatctaa aaaattattg acataacgcg ctccgtgagt ataaacgctc
    10981 tgttttgaag cacctctgtg gttgtttctg ttacctgtct tcctgaaaag ggagtaagca
    11041 gaaattcaga gggaagattt aacaggtaaa ttcttaggag gaaaaaatgc ctgaaatcag
    11101 agatttattg aaagcaggtg cccatttcgg tcaccagaaa cagagatgga accctaaaat
    11161 ggcgccttac attttcacag agcgcaacaa cattcacatc attgatcttc aaaagacaat
    11221 ggaacttatt gaagatgcaa agaagttcgt aaggaacatc ggaatgaacg gtgaaacaat
    11281 ctttcttgta ggaacaaaaa gacaggcaca ggaagttatc aaggaagaag ctgaaaaagc
    11341 tgaacttccc tatgtaaact accgttggct tggcggaatg ctcacaaatt tcacaacgat
    11401 caaccagtca gttaagaaac ttcagaaatt tgacgaaata ctcaattctg aaaaaagacg
    11461 gctctataag aaaaaagagc tttcagacat agataaaaag aaagctaaac ttgagaaaaa
    11521 cctttcaggc atacttagaa tggacaagat gccgagtgca atattcatag ttgatccggt
    11581 aaaagaacat cttgcagttc acgaagcaaa gatcctcgga attccggtaa ttgcaattat
    11641 tgacactaac ggcgatccga caaagatcga ctttgttatc ccggcaaacg atgacggaat
    11701 gcgttgtgtt tcgatcatta catctgatat agtggcggct tatatggaag ggcttgacat
    11761 ctataagcag aagatagtga ctgagcagaa agacagaaaa gataagcccg caagaggaga
    11821 aacaagaagt gttgccggaa gaaaggtcag agtgaagaga atagcaactt ctgaaaatcc
    11881 tgacgaacag accgatgcac acgaagaaga tgtaacagag aaaggggagg aataatatgg
    11941 aaatttccgc atcattggta aaagacctca gagaaagaag cggcgcagga atgatggatt
    12001 gcaaaaaagc cctccaggaa gctaacggga atatggaaga ggcaatgcag atacttaagg
    12061 ataaagggat agcaaaagcc gcaaagaaag ccggtcgtgt tgcaagtgaa ggaactgtgc
    12121 tttctataat tgaaaactcg aacgggcttc tttttgagct taactgtgaa acggattttg
    12181 cggcaaaaag cgaaggtttt aaaaatcttt ccagagaaat atcgggctat ttcatgaaaa
    12241 atgcatctga ggaaggggtt ctcgtttcaa tggaagataa attctatgat gcaaatgttg
    12301 aaaaacttac aacagacgca gtggcaacta tcggtgagaa cataaaaccg agaagatttg
    12361 taaaatattc aggaagcgac agctctctca ttcattccta tattcatatg ggtggaaaga
    12421 tcggagtcat gctcgaagtt cagtcctcag ataaagatgc tgtcaaaaaa ccggaagttg
    12481 ttgagatgag cgacgggatc gccatgcaga ttgcctcaat gcgtcctgac tgtgtagatg
    12541 aatcttcatt tccgcaggac aagctcgagt ctgaaaagcg tgctttcctt gcacaggtaa
    12601 tggaaatggg aaaaccgcag aacatcgctg aaaagatagt tgaaggccgt attaagaagt
    12661 tcatagcaga gaatacactg cttgagcagg cgtttgtaat gaacggcgac ataaaggtaa
    12721 aagaccttgt aaaagagacc ggaaagaaag ttggagcgga gcttaagata gtaaggttcc
    12781 atcgttttga gctcggagaa gggattgaaa agaaggaaaa taattttgca gaggaagttg
    12841 ctgcacagat taaatgatct gatgaaaaaa gtcttttcag atattttcag aggggcgtcc
    12901 ttaaaaaaga cgctcctttg tttttttatt ctttccggat attttttgtt gtccggcagc
    12961 gaagcaaagg tttatacaaa agatcagttc gtggaagttt tcattgaaaa agccgatata
    13021 atagaatctc ttaaatataa aattgaaagt gctcaggccg attacaataa agttctttcc
    13081 cagtattttc caaagataaa attcactttc ggtgtcggtc ctcatcctaa atatgaatat
    13141 gaaagggcga ggatagaaga ggatggtcag ggtggataca cattcattga aagtgactgg
    13201 atcaagagtt attacgatct tagcgagtat ggtgttgcga taaggatgaa aggcgatatc
    13261 gttcttcctg tctatacatt cggaaagatc aaaaacggtc ttgatgtcac caaagctcaa
    13321 attgaagcaa aaaaagcaga ggcagcgata ggagagctca aactccgcaa agaggcggct
    13381 gtattctact ggtcatgggt catggcttca gaaatgttcg caactttgga gccggcgctg
    13441 caacaggtcg ataaagccga agaaaagctc aaagagatgc tttttgaaga aaaagaaggg
    13501 gtcagacaga aagacctgat aaagcttaga attgaaaaag agaaacttgc ctacagacac
    13561 aaaaaactgg tttttcagat ggagacactg aaggaagtta tttcgcagat acttggcgaa
    13621 aactggcagt tggcagactc ttcaatgaaa gttgttgaat ataaaaagga atatgaccag
    13681 atcgttgatt atatgttttc tgaaagccct tattcaaaat atcttacaag cggactgagt
    13741 gcatacgaga acctttatga gcttgagatc tcaaagatcc tgcccgatct ggggatagca
    13801 gggtatttca catacaaata tacaagttct gtttatgaga agaattatcc ataccccgac
    13861 agtccttaca acggatggga tggagaactg ggaataggtt ttgttttcaa tctcaatttt
    13921 gttgagcagg ggatgaacat aaaaaaggca aagtccgaat ggcgcgcaat gaaagcacag
    13981 gcatcatttg caaaaaagag tgctccattg atcataaagc agaaatataa tgaactgaag
    14041 tcacttgaat cacaggtgga gcacatgaaa aatgcccgta aattctcaaa aggatggatg
    14101 acatcagaat ttacgaacta tgagagcggc tttaacaata ccaatgatct tatcgatgcg
    14161 gtgaaggttt tttttgaaaa cgaatacctt tatatccaga gcatttatga ttataatatg
    14221 aaagttgaag atcttattga atatacgggt gcgaggtgat catgaagaat tcggtttttc
    14281 tgttgtttct tctttttctg tcgggagttg tttccgctaa tgaaacaaag aaggttgaaa
    14341 atcctgcggc tgctgttgcc gggaatgaag agttcaatgt caatgaagct gtgaaattcc
    14401 ttgagtcaaa agagcagata cttgaaaaat gggcgaatgt caaaccgaag aaagggtcag
    14461 cccagttcaa gcagaaagat gccgacatga aaaaggcggt ggaggagatc atagattata
    14521 aatttattgc aagttacata attggcgaaa aatgggaaac aacacccgaa gagaaaaagg
    14581 aagagctgtt cagcaagatc aaggaactgt ttactgaact ttatctggag gacactttct
    14641 acaataaaag ttacgaaaag aagtatattg ataaagggat tgagaaaatg tacataaaag
    14701 gtgttccgca gagtgttttt gttacgacag aagttcaggc cgctctcaaa gggaaacctg
    14761 taatctatga acttatctat catcttcata aagttgacgg atcttacaaa gtgtttgaca
    14821 ttgagcttga cacagtcagc atggttagaa attatcggga acagtttatc aagaatgtta
    14881 aagctcagtc ggttgaagaa cttataaaaa tgatcgataa aaaggtcagg aacaggaaaa
    14941 aagaaggttc aatatttaaa gaaaagaaag tttcgtaagt aattgaaaga acacatttta
    15001 atccattcat gctgtgctcc gtgcactaca gcggttctcg agaaaataaa aagcggttct
    15061 gatcctgata tttcagtttt ctattacaac cctaatattt ctcctgaaac cgaagaggag
    15121 aaaagattta tagaattaag cagatatgtt gaaaaaagat acgggtcagg gatagaggtc
    15181 atccgcggaa aatatgataa ttatgaatgg aatcagatgg tagatcctgt ttcgaccagt
    15241 ggggagaggg gtttccggtg taagatctgt tattatatcc ggcttcttga aacatttaga
    15301 aaagctactg aaataaatgc gacaacggtg acaacgactc taagcatcag cccatacaaa
    15361 aactatgagt ggattgatga gatagggatg ctgttgtcgg aaagatttga tataaaatgg
    15421 ttccttgaga aatgggatta caaaaggtcc attgaattgt caaaagaata cggattatac
    15481 cgtcagaaat actgcggttg tattttcagc gcaaaagaga gagaattgag aagatagttg
    15541 ataattactg caggagaata aaatgggaaa tgtagggaag ccggttgtag aaggaaagaa
    15601 tattcagctt aaattgaaaa atgatgatgt tgcggcaggc aactattcga attcatttgc
    15661 gataacattc aatgaaaatg aatttctaat tgattttatc atgatgcagc cgcaggccca
    15721 gatgggtctt gtaaccgatc gtatcgttgt tactccaaga aaggcggcgg aacttgcaaa
    15781 agctctcagt gatgcggtga aagcgtatga aaatatgctc aaaacaagaa aagacaatat
    15841 tcagtaacgc tgaatgtcag acatttctta tttcggagag atcgcggcaa taagtgcatc
    15901 gctgtgctgg agtattacat ctttagcttt tgagtcggcc ggcaaaaggg tcggttcgct
    15961 gcaggtaaac ataatccggc tggttcttgc attgtttttt ctttccggaa tgtcgttttt
    16021 tgcgaatggt cagttttttc cgttcgatgc aacaccctct gcctggttct ggcttacact
    16081 ttccggtgtt gctggttttc ttatcggtga tcttctttta tttcaggcac ttgttgtaat
    16141 cggctcaaga atttcaatgc ttataatgac actcgctcct gtttttacgg caatacttgc
    16201 aaagataatt cttggtgaag agcttgccgt gacatcattc ctggggatga tgctgacgat
    16261 ttcgggaata atttttgtga tttcagggaa aaaaggtggt caagagagga aaattacaaa
    16321 atatggtctt tctcttgctg tcggaggtgc aatgggtcag gcactggggc ttgttttaag
    16381 taagctcgga atgaaagatt acagcgcgat atcatcaaca cagataagag tgattgccgg
    16441 gatcataggg ttttcgatca tttttacaat gctgaaaaag tggggagttg tgatcagttc
    16501 cataaaaaac atttctgcaa tgaaaaggat aaccacaggt gcttttttcg gcccttttat
    16561 gggtgttaca ttcagcttgc ttgcagttca gcacacaaat gccggaattg cggcgacgct
    16621 gatgtcgtta gcccctgtgt tcataattgt tccaagcaga ttcatcttta aggaaaaaat
    16681 aaaactccgc gaaattgcag gggcagttat cgctttttcc ggagttgctg tgatatttct
    16741 gttttaagaa tcttttttta tcgacttagt tcagagagtt ccaaaaagac ggtcgcctgc
    16801 atcaccgagt cccgggagga tgtatccttt ttcgttgaga cggtcatcga cttgagctgt
    16861 gtagatcatg acatcgggat gttcttcttc catctttgca agaccttcgg gggctgaaat
    16921 gatggtaagt acctttatgg acaagggttc ttcccttttt atgatgtcaa ctgcggcact
    16981 gagcgaccct cctgttgcaa gcatcgggtc aagaatgaaa acatgccgct cagaaatgtc
    17041 acggggaagt tttttgtaat attcgactgg ttcaagggtc tcttcgtttc tgtaaagtcc
    17101 gatgtggccg actttggcga tgggtagaag ctcctgaaga atatcggcca ttccaagtcc
    17161 tgcacggagt attggaatga gagcgacagc gttatcctca agaatgaacc ctttcgtttt
    17221 catgagcgga gttgttatct caacttctct gacagggaga tctcttgatg cctcatacat
    17281 cagaagaata gaaatttcat tcaaaagctc cctgaacatt tttttgtcag tgtcgtgttt
    17341 cctcatccag ttgatcttgt ggcttattaa tggatggtca atcgctgtaa acattttaaa
    17401 ctcctgaaaa tattaaataa atgctttcat gactataaag aatattccgg cagtaaaata
    17461 tgccagacgg taaaatctcc tttcgttgag gaaccgttct tttgacagat cgttcctgca
    17521 gaacataagg cggtatgcaa tgtgtactgt tcctgcgaaa atgaaaggcc agttcagcat
    17581 tatgtcggtg atccggaaca ttgagagagt caggagatac agagtcgaaa aggtgaaaac
    17641 ggaaaacgag acccgttccc agattttttt cccgaaatgg accgctccgg tgttgacatt
    17701 catttttctg tcagggtcat gatcaataag ttcgtgattg agatgtccgg cagaaaaaag
    17761 gagtgaaaag tatattgaaa caaggatgga gctgaagctt gttctgtcaa ggaccgaata
    17821 tcccatttga aagtggatcc cctgacccag aaagtggatc acagtaccga gcacaggtct
    17881 gtatttaaac ccttttttag gccagctgta aagagtccaa agaaaaaagc ttacaaggga
    17941 aagaatgaca aaatatatcc ctgaaatgga aaaagtcaga ataaaaagga tgcctgaaac
    18001 agcggttgcg gtcataaaac ggttcttttt atcctttaat cctgtaagcc gttcattttc
    18061 accatcttca tcatagccgg cccaggcgtt gaaagcgtat attgcagtac agagaaagaa
    18121 cagccctgta ataaaaatga aaacttcgga agaaaaaagg ttttcaaaat tcttcatggc
    18181 aaagagagtc cccataaaag ggaatccaag catcagtgaa acttcagcaa gccgtgcaga
    18241 tttgatcaga ttagcgatct ttcccattaa aactcccgga aaatctgtat ttttataaac
    18301 cattcgggct aaatagtaaa gtattgttaa aaatttgatt agacacatat ttttattatc
    18361 attcaaatga attttaattc cggaggggaa aatggacgct aacgagatca tcaggatcat
    18421 cagtgaatca aagaaaagga cacctgtgag ggcattattt aaaggggcag ttgaagaagt
    18481 cgatttctca gattttgcag agttttatga aggtgttgat ttcattgaag tttacgatga
    18541 acttaatgtc gtcagggaat ttgcagaaaa aaacagggat gtcaaaatga cacggctgga
    18601 gatagacaga agaaactctg ccgttccact ggccgacctt tcaaaataca atgccagaat
    18661 tgaacccggt gcgatcattc gcgatatggt cgagataggt gataaagctg taataatgat
    18721 gggagctgtt ttaaatatcg gtgcgatcat cggtgaaggg acaatgatag atatgaatgt
    18781 cgttgtaggc ggaagggcga tagtcggaaa gaactgtcat atcggcgcag gcacagtact
    18841 tgccggagtg gttgagccgc cgagtgcgac ccctgtcgtt gttgaagata atgttctcat
    18901 gggggcaaat gccgttgttc ttgaaggggt cagaatagga gccggctctg ttgttgccgc
    18961 aggagcagtt gttaccgcag atgttccgcc aatgacagta gtcgcaggcg ttccggcaag
    19021 gataatcaaa aaagttgatg ataaaacaag atcaaaaaca gaactcgttg aagcactcag
    19081 gaatctttaa actaactctt tgatttaaat agaaaaatct ttctggacag tttattgcta
    19141 tttagttggt agaattattg tttattgagg atttacgaat gaaaaagtta cttgttacag
    19201 cattttttgt ttcctttttt acactttctg gtctgcctgt tgagattgtt attccagaca
    19261 ttgagttcat cgaaggcgac gaaggtgtta tccctgcgat agagggttac gagcttgacg
    19321 gaacagggga gaaggtgatc cttccctata aaagagttgt tttttcagga aagataaagt
    19381 ctgtcgagat cgttgaaaaa agggaaataa cattaaccgg tccgcttaaa aaagggatgg
    19441 ctctatacag actgaacgat atgcagaaag ttgaaaaagt tcagtttgag cgcgcatatc
    19501 ttccaactgc ggcaaaattc ctttttcaga aaatgccgac ttatagcggc gatatcaaag
    19561 agttttccgc cgatatctat cctgtcattc ctgtaagtga cacgaagatc attaaaatag
    19621 acagggtgag aatagatctt gaacaaagct ctcctgtccc gatgggcccc acaaaaggac
    19681 gcgacacact gctgatcctc acttctaaaa aagtcgtttc ggaatcaaag gaactgtata
    19741 atttcatttc cgtaaagaga gctgacggtt tcaaagttga ggtggcaacg gaagacgatt
    19801 ataatggtgg agaccttaaa gggatagaaa gagttcataa aataagggat tatcttaaag
    19861 acagatatag agaattctat tttcttctga tcatagatga tccattaccc ggcggcaaag
    19921 gtgttcctat ggtcacaaca aggccggaca tacttgaaga aaaaagctac gagcaggttc
    19981 ctactgatat attctatgca gaaataagtg aagaaataga tcggaacaac aatggaatat
    20041 acggtgaaag ggaagataag atagagtttt catttgaata cattgtcgga agaatacctg
    20101 tatacaatga caggatagac aatgttgata aaattctgac aaggactatc aaatttatta
    20161 aggaaaaacc ttcggaggca acatacagac agagatttct gtttcctaca acgatagcat
    20221 attatccaaa tcaggacgga cagatgttcc tgccgaagat ggacggcggc tatgttgtaa
    20281 agtttctgaa ggagaatgtt cttttggaca ttttcggttc aaagacactt gttgaagcag
    20341 caggcgcaaa cccttccgaa tttgtggatg aagagcccat aaattatgca aatgtgttga
    20401 aacaatggaa cgaagggtat ggcgcagtat actggatggg tcatggaatg ccgacatatt
    20461 ctgtaaggac tgtctggaga ggtgacatca acagcaacgg ttatgcagac agttacgaat
    20521 tgaacgggga aaattttgtt gacagcgata tgactgaaaa gatgacacac gatgcaccgt
    20581 tcgttttcca ggggagttgt cttaacggaa cgataacgaa taacaggaat cttgcatata
    20641 tgactcttct taacacagca gtcggcgttg tcgggagttc gcaggtttca tacggaacga
    20701 tattcagtga ttacgaccag agcagccagg atctgtttgc atacggaacg gtttttattg
    20761 aagcaattgc ggataacaaa ccgcctgcac agatccttca gcagaaaaaa cagagctggt
    20821 caagtggttc agtgctcctt acaataaaaa tggagacgaa ctatttcggt gatccttcac
    20881 tcaacatgaa tatgcagaaa tgtgagattg atgaagactg tgatgatctt attttctgta
    20941 acggcacgga agtgtgcagc aaaggatact gtgaaaagaa tcacgacgcg ataccgtgcc
    21001 ctgcttctct tgacccgtgt atgcccaata tttgtgatga aaagacaaaa tcctgtgctg
    21061 cacaggcgag tccggatgga ttttactgcg gaactattga tgatccatgc ttcgaaggaa
    21121 tgcgctgcaa tgaaggaaaa tgtgttcaat ccggacccaa ggattgctca tcttttgatt
    21181 cgccatgttc aaaaggtgtg tgcaacagtt caaacggtct ttgtgaactt gttgcagaaa
    21241 acgaaggggg agtttgtgaa gacggacttt tctgcacaat taatgagaaa tgcattaaag
    21301 gtgtgtgtgc tggtgaagat atcgacttgc cggaacctgg ttcgtgctca aaggttgaat
    21361 gtgataacgg ttcagaatct ttcacacagt tgatagacca atcactcaac tggacctcat
    21421 gtgaaaccga tgacggagca aagggaactt gttattatgg tgtttgtaaa gagaatgaag
    21481 atcaggaaaa gaccggctct tccggctgct ctgtcatatt cttttgatgt tttttatatc
    21541 gtcaacgacc ttgcgcgtca tttcaagagg atgataattc gggaaaatac ctgatatttt
    21601 cagggcctta gcagcagtaa taatgtttct gtccattgaa tattgtgaga aaaaatagaa
    21661 aggtatttca gaaaattcct tcttgtgctt cagtgcggca aggaatttaa taggacttat
    21721 tgtgctttca ggcatatcaa ggtcacagag aataaagttg agcgcctctt tcttgtcttt
    21781 gatgatctga gccgcttttt cgaacagatc agctttgtat gtttttacat taagccggtt
    21841 gagtttgtca gaaatatcat cacagtcgaa tctttttctg ctgataatga gcccttcaaa
    21901 aacaggtgaa ctgcttgatt gcggcatggt gtcaagctgt tctatctttt taagtgcaag
    21961 cagaagttta tcggggaaga aaagaaggtc tttgatctgt ctgtaagctg ttgctgtcgg
    22021 gattttagat actcttgtca tgtggtcata agtgtctgca aggagaagta tcaacgagcc
    22081 ggttggaact tcattcaagc tgagaccttg gggaacgcca gctccattat aggtttcata
    22141 catattggca agatagctca gtgtgagttt ggaaagatct acattgctga aaagctttga
    22201 ggcaataccg gcatatttta taagtttctg gttgtcggtc ttctgtttga tatcaagtgc
    22261 ggtaaaatgg ttctttttgc ctacatcgtg aaggtatgaa gcaacaagca gatcatgaag
    22321 ttcaacatca gaaagactca tttcgtgtcc aagttcctgc gataaatttg caactctctg
    22381 cgtgtgaccg aagtaggagt catctctgga agtatcaaga agattggaaa aaactctgat
    22441 tatttctatc atctgcccct gctgatctct tgaaagaccc ggcattagac tgatcataga
    22501 agctgttacg gaagttgatt cgatcatttc gccctgatta agaatggctg aaaatgtggc
    22561 attgttctca tttctgttcc tgttatcttc agtgatgatg ccttgtatgg tctcgcccat
    22621 actgctgaca gaatctcgtc ctattattga ttcactgccc ggcagaagga tgttcatttc
    22681 gacacctttt ttgacaaggc gctcaaaaga tgaaagatct cctttatact gtttatatat
    22741 cagagctttg attgcgtttg atgtagttac aacgggcaca attgtgttaa cccctgtcag
    22801 gagcaccttg agttcatcaa aaagtgcgat gttctgcggt tcatgagtga ggagtgtcag
    22861 acggaagttg ttgaaactgt atttcagagg gtagacattc ttttcaaagg cgagtttttc
    22921 cgggatcaca tcaacgggat gctgagccgg gatcttttca agtttttcgc ttgaaatgta
    22981 ctggacattg aacctttttg aaagatagtg aagcaggtct gtttctgtta taagctcctg
    23041 agataacagt atttctccga gatatcccgg gcgtttcttc tgatattcaa gtgccgctgc
    23101 aacatctttt tccgttacaa gtcctttttc aatgaaatac tgtccgactt taactgccat
    23161 tttaacttcc cgaaactgtt aaattatcaa aaactacagc atagtccgaa aatatatcag
    23221 aactgcattt ccaaatcaat ttttaaaata actttccttg acataatggt atccgccttg
    23281 aaattcttcc aaataatgat gaaataagtt atacttcatg acgcattgaa caggagcgtt
    23341 gagaaagttg agaaggataa ttgctcagaa taaaaaggat ttaaaggcga atgcaatcat
    23401 tccacggagt aaaaacatat gtacttatgg cgacctctat gattttatgg cgacacctat
    23461 gataccagac ccctatgatt tgtgacccct atgatttgaa agggaataac tgacccctat
    23521 gatgcaaagt aaatcttcca aatatgatga aacaagtgaa ttaagaatac tacgatggtc
    23581 tgtatacgat gttaagaaac tttgtattct ctgattgatt ctgcgatgat attcagtttt
    23641 tcgggtgtga agtcatttct ataaaatatt ttgaaaagtt cggctatcag atctctttca
    23701 ctcatttcgg ggtgcttttc acgaagctga tccagaataa tggttcttga aagctctgtc
    23761 agttcaagcc atctttcaat tttttcagca tcagatcttt tgttaatgat ctcaagctgt
    23821 ttttgcaaaa tgtgtttcgg cgtatcattc attgtcaagc aacccgaaag tttgtaaatt
    23881 aagcgcttca caccattctt ttatatatcc gattttcttt tcagaattat ccagaagatt
    23941 cctgatgtcg ttcatctgtt tttcactctg gtaatcctga atccacatta tttttgcaat
    24001 tatcagatca ttaagatcaa ttatccggaa atcttttccg ctcccctttt taatctttct
    24061 gtttttgaaa gcaagcttgg aatattcaga attttttctg atgataaaat caatcttaaa
    24121 gccggtctga ttgtggatga cattgaacat ccctttgcgc ctgacttcac ttctcacagt
    24181 atctttatca atataactat caggaaatat ggcaacaaat gcatcgattt tttcttcttc
    24241 aagttcgatg acaatatcaa catcaagagt cattcttgga atacaataaa aattcagggc
    24301 gatgcttccc gagatcatat attcaactga attctgttca agtctatcac aaacatcgtg
    24361 aatgaatttg ttaatatcag gattcattta cctttccata aactaatgct ccgaaacaat
    24421 gataccttca caccgttttt tttcaattaa aatgaaatgg aatttctcct ccaaaaccag
    24481 ctaataataa aaataactta aaacaaataa aaaaagttgc ttttggaaaa agtttgtggt
    24541 aaaaagtgga atcaaatggt tagagagtgg tgacaaatgg ttacaaaagc tttttttcac
    24601 ggcagaaaag attaccggct cgacccgaag gggagaatcc ctttcccgtc tttgtggtat
    24661 gcgcctctgg ggcttgaaaa ttccaacaaa gttgtagttg ccaagggtct ttcagaggag
    24721 aagtatcttg agatcttttc ggttgagagc tggacggagc agatgaagat catcgatgag
    24781 tctccgatcg gaccggcgag gaacaaaatg ctcaaatggt atgttgcaac tgctgaaacg
    24841 gtggagcttg acaatcagaa cagattgcgg ctctcaaaga acctccttga ttatgccgat
    24901 atcaaaaaag atgtcgttct ggccgggagc atagaaacag cccagatatg ggcaaaagag
    24961 gtctttgatg cttcacttgt gatggacgat aacgattttg atatgatgta taacttcatg
    25021 aacgatgcaa ggaagaaaat aagggaaaaa ggggaaagat gagctttgtc cacaagccgg
    25081 tactcatgaa cgaggtcctt aatttaatga atcttaatga tccccgttca tatcttgatc
    25141 ttactgcagg cggcggcgga cacgcaatgg caatgataaa taaatatcct tcaatgaaag
    25201 cggttcttct tgatagagat cctgacgcag taagtcattt gaaggagaaa tttgcaggga
    25261 tggataatgt gactgtcgca aaatcaagct tcagcgaact ggataaggtt ctttttttaa
    25321 tgaagagaga cgatgtcgat attatttttg ccgatcttgg tgtcagtagt catcagtttg
    25381 atacagctaa aagagggttc tcaatacaga aagacggacc ttttgacatg agaatggact
    25441 ctgagagcgg aatgaccgcc ctggaataca ttaaaaaaac cgaggagtcg gaaataaaga
    25501 acattttaag catatatgct caggaaagag aagccggcaa agttgccaga gttcttaaaa
    25561 aatgtgctga agaggggatg acgacgactt cggagtttgc aggtgcaata agaagtgcga
    25621 aaaaatacgg taaacagggg atcgatccgg ccacacaggt cttcatggct ttaagaatgg
    25681 ttgttaacga tgagataggc cagcttgaaa aaatgctcag aaagggattt tccaagcttt
    25741 cagtaaacgg aataatggga attattactt ttcattccac agaggaccgg gctgtaaaga
    25801 acttcttcaa ggatagaaag aacaatgttc cttattatat taatgataat gacaaagttc
    25861 catattcctt ttctcctgtg acgatttcag gtcctttttc tgcaggtgaa gaagaaattc
    25921 gtgaaaatcc gagggcaaga agcgcaaaac ttagacttat ttcgaaaagt tcattttata
    25981 attaagaggt gtgtcatgac gggatttgtg catgttgaag gagaaaaggc cgaaagatca
    26041 ggaaaagcga tcttcagcgt aagaaaggca aagacaaaaa tgatgctttt ttctaaagat
    26101 gtggtagttt tcattgttat ggtggctgtc gccggctcgc tgatgctttt taacagctac
    26161 ataaaagcgt tcagaaacag tcagaatgtc gagatagtca agcgtattga aatgaaaagg
    26221 gagctgatac tgaaaaagaa tgaagtcaac ggagcataca ttaaaatgag ctcgtctgcc
    26281 gaactgatga aaaaagccga tgaacttcaa ctgagcgttg ttacacttga taaagtcatg
    26341 cagatccagt agcggagtgc tttatgccca gagagaataa gctatatctg ttttttgcag
    26401 tattcatctc tgcggcattc atccttttaa tatacagagc atacattatt cagataatca
    26461 gacatgaaaa acttcaggaa tattcaacga atgatgaagt tgttaagata ataaaaggaa
    26521 aaaggggcgc gatttatgac agaaacggag agccgctggc ggtaagcgaa ccgaagatag
    26581 atattgcggt tgattcaaag aacataattg aaaaagatga gatggcggtc attatctcta
    26641 aatctttaaa gctcgacatc aaaaaggttc atgaaatact caacagggaa ggacactttt
    26701 attacctttt aaaaaatgcg gaatatgaca gtgtcaaagg gctgaaagaa cagaagaaca
    26761 atatcagaaa aaaactgaaa gaaatgaaaa tgtccaagga gatcaacaag gagctccagg
    26821 ataagtattt gaatatgttc agagattttg gtttcataat ttatcttgaa ggataccaga
    26881 gaacatatcc gcaggggaag atgcttgcca atgtgctcgg gttcgttcag agaaatgacg
    26941 atgtcggtct tgaggggatt gaaatgagct atgacaaatt tcttgccggg aaggaaaaga
    27001 aattaagacg acttacgatc cccagtacag gagaaagcac acttgagatc gaaaaagagc
    27061 ttgacgattc atccagtgcc gatatttatc tcacaattga ttcaaggatt cagttcatag
    27121 ctgaagaaga actgacaaaa atgatgggta agacaaaagc gaactggggt ggagttgttg
    27181 ttatggagcc cgcaacaggg aagatccttg cgatggccaa ctatccgacc tacgagccgg
    27241 acagatattt caaatattct cccaaagaaa gaagaaatta tacgatagcc aacctttttg
    27301 agccgggatc tacattcaag gttttttcga tacttgccgt gctgaatgaa aatcttgcaa
    27361 agccaggtga gatgatattc ggcgagaacg gaagattctc gttcggcaga agatgggtaa
    27421 gggattctca tccctatgaa tggatgagta ttagagatat cgttataaac tcaagtaata
    27481 tcggcactat aaagctcgcg gaccgtcttt caagggaaca gctttatgat tatttcacag
    27541 atttcggttt cggaaccaag acaaagatca atcttccggg tgaatcgttc aagccgatca
    27601 gacctgccgc aaaatggtat ccgatcgaca aaggcaacat ttcattcggt cagggtattt
    27661 cagtaaacat ggttcagatg gccagagcgt atgcatctgt ctacaacggg ggagtgctct
    27721 ggcagccggt gatcgttgac agaatagtta acccgagtaa cgagaaaatc ctttttcaga
    27781 ctgttcctga acccaaaagg atcgctttca aattcagctc ggacaaaaga atggtcagca
    27841 tgcttgaagg tgttgttgaa gagggtactg caaaaagtgc agcacttgag ggaataaagg
    27901 tcggaggcaa gaccggaact tctcaaatat ttgattttac tgctaaaaag tattcatggg
    27961 acaaggttgt ctgttctttt ataggggcag ttccaagcaa tgatccagct ttcgtaataa
    28021 tggtggtgat cgaacagccg gaaggccgtg agttcggagg aacggtggcc gcacctgtct
    28081 ttagaaatat tgcggcgaga tctcttccgt tgtacggcat ttatgtcgac agaaaggcaa
    28141 aggataagag cattatccct gatatgcccg gaaaagatat aacaggtgtt gcagtcgaag
    28201 cttcttcaga aaaggaatcg gaggaaagcg gagcgatgga tttcataaaa attccaagtt
    28261 tcataaatag tgattcaaca aaagctcttc agacagcaaa caagacaggc cttgatgtcc
    28321 ttttttcagg ggatccgtca agaaagatcg taaaccagta tcctgcaccg ggcgaatttg
    28381 tccctttcgg tacggttata atgcttgaaa cagaggaacc gcagataaat gagaacaact
    28441 gattttatta aacttatttc aagtcacatt gaaaaagaga tgtcaacggc agacgaaaga
    28501 gaactcctca gaataactgc cgattcaagg gaagttgatg aaaaaacagt ttttttctgc
    28561 ataaaagggg caaaagctga cggtcacgat tttgccgaac aggtgataaa aaagaatgca
    28621 ggtctcgtcg tgggtgaaaa agagctgaag atggaaagat atgtcagagt taattctgta
    28681 aaagaggttc tcaagctcat ttctcctgcg ttttacaact atcctgactt aagaatgaaa
    28741 atggcggctg ttaccggaac aaacggaaaa acatcaacga cattcatgat agagagcatg
    28801 ctggcgggca ttttcagatg cggagtgata ggaactctcg gttacaggta cggaggcagc
    28861 acttttgatg ctccgaatac cacgccgatc aactggaagt ggtattctct gcttaacgaa
    28921 atggagaaag ccaaaacaga agttgtcata tctgaagtaa gcagtcatgc aattgaagag
    28981 gaaagaatag agaaaacgct ttttgatgtt gcggtattta caaatctgac aagagaccat
    29041 ctcgattttc acggtgatat tgaaaattat tttcaggcaa agaaaaaact tttcacaaaa
    29101 caccttaaaa aaggggctgt atccgtaata aactatgatg acattttcgg gaaaaggtta
    29161 tatgaagaga tctgcgatag tgtcaaatgt ataagaatat cggaatcctg ccatgatgca
    29221 gagcttaaga tatttataaa aaagctcgat agcagtggaa gtacagtgga tatttcgttc
    29281 gagggcttat gcaaaaccgt taaaattccc cttctcggga aatataatat ctacaacaca
    29341 gcatgtgctt ttgcggtgtg ttcggtttta gccggcaggg agatggcgtt tgaatctatc
    29401 ggaagcaggg tttcagtgcc gggccgtctt gaacagatcg gagaagaatc gatatttgtg
    29461 gattatgccc attctccgga tgcactcgaa aacattctta aaactgtcac agatctgaaa
    29521 atattcaaaa gggtcataac tgtgatcggt gccggtggcg acagagacag aggaaaaaga
    29581 cctttgatgg gtgaagttgc tacaagatac agtgatattg tcatattgac ggacgataat
    29641 ccaagaagcg aagatccgga aaacatcatt agagaaattc tggcaggttg caacccctca
    29701 gagagtgtga tcgagataat taacgacaga tctgaagcta tcaggagagc ggtctttata
    29761 aaaacaaacg aagatgcatt gatagtcgca ggaaaagggg cggagaatta tcagatcact
    29821 catgaaggga aaagattttt ttcagataga gaagagatcg aaaaatatct aaaggagttg
    29881 cacaatgttc acaacagcac tgattgaaaa ggtattaaga gctagcggta tcagaaaaga
    29941 atttgcaccg ggaattattt caaatgtttc aatagactca aggagttgca ggaaaaattc
    30001 cttttttttt gcattcaagg gagaaaatgt tgacggacac gattttattc ctgcacttct
    30061 tgaaaaaggt gttctctgtg caggggaaaa ggatcttcct gaaaacgatc tctacataaa
    30121 agttccaaat gttctcgatt ttttaagtgc acttgcaaaa gaaagaagaa aactttttaa
    30181 agggactgtt tttgcaatta ccggctcaag cggaaaaacc tcgacaagac agctcactgt
    30241 atcgatgctt caacttcttt ctaaaagtgt ccatgcgaca agcggaaatc ttaataatca
    30301 cataggttta ccacttgtga tactgaacac tccgatgaat attgatgctc ttgttctcga
    30361 aatggggatg aaccatgccg gagagatccg ccatcttacc gaaattgccg accctgatct
    30421 ttctgttata acaaatatag gtacggcaca cattggaaat ttcaggtcac aaaaagatct
    30481 tgcctttgca aaacttgaaa tatttgaata ttcaaaaggg attgttgtgg ccgatacagg
    30541 aaatgagttt ataaaaaaat gggtcggaag caacagatct aagagaaaaa tagttgaata
    30601 ttcatcttct gattctgaaa agatcgctcc tgagtttgat tatctggaaa agtatatggt
    30661 cgaaaacctt tatactgcat cggtaacggt caaggcatcg gaagggataa taccagacct
    30721 tgggaaagtt gtaacaaacg ccagttttcc ttcgatgagg ggcgaaataa ggaaggtgga
    30781 aaacaggaca tttgttgtgg attgttataa cgcaaacccc ggatcgatga gaagttccat
    30841 tgaaaatttc tatgaaaaat acagcagaaa aagcggttct gaaacatacc tgattctggg
    30901 cagtatgttc gaactcgggg aattttcaaa aaaaatgcac gaagaacttg taaattattt
    30961 aaaaaatctt aacctattaa aaagggtttt tttgataggt tgcgagttcg aaaaaacaag
    31021 acctggtttt ttgaatgaaa aaaaggtgct ctttctgggt gatatcaccg atattccgga
    31081 acatctcccg aaagaggggg ttttcctgct gaagggctcc agagggaaca gaatggagaa
    31141 gattttcgat ctgcttgaaa agattggagg ggttgattga aagcgttagt tatcggactg
    31201 ggggtttcgg gcacatcatc tgcaaagttt ctaaaaaagt tcggaaatga agtttcaatt
    31261 tatgacgaca acagaggtgt gctggataaa ttttccggaa catatccggt ttacaacagg
    31321 gatgaatctt atgacacggt gataataagt ccgggagttc cgtcagatca tccggtagtt
    31381 atggaactca gggctaaggg aacggaagtt atcggtgaaa tggagcttgc cggaagatac
    31441 ctgaataacg agaaagtgat cgctgttacc ggcaccaacg gtaaatcaac aacagtttca
    31501 gttgcctgcg ctatgctgca ggaggcggga ttcaaggcgt tcctttgcgg caacatcgga
    31561 gagccggtga tagaaggcgt caacagggat tatgactttc tggttatcga gatcagttct
    31621 ttccagcttg aaacactcaa aagcatccgc cctgatgttt caatgatact gaatgttaca
    31681 ccggaccacc ttgacagata cagcagttac gaacattatc ttcttacaaa agttgaactt
    31741 gcgaaactca ctaaacctga cggtcttgtt atccttaacg gaggggacga agcacttgtt
    31801 gcggcgtgta gaccggttaa tgtcgcaaag aaatatttct cggtgaaagg tacgggcgac
    31861 atctcataca ggaatggaag catctatttc ggcaacagcg aattgaagat agacgatctt
    31921 tatctcaaag gacttcacaa tgttgagaac ataatggcct gtgtacttgg agtcacccct
    31981 tatattgacg atgtgaatgt attgagaaga tgtcttaaga atttcaagcc ccttgctcac
    32041 cggacagaat tcgttgatga gtttgaaggt gttgttttta ttgacgattc taagggtacc
    32101 aatgtcggtg cagttgaaat gtcactggcc ggttttgaag acgggaaagt tgttcttatt
    32161 ctcggcggag ttgataaggg cggaagttat gaacctttga ggatacttgc tgaaaagaag
    32221 tgcaggggtg ttgtgctgat aggcgaagcg aaagataaaa taaaaagcta ttttgaagga
    32281 ttcagggatc ttgaacaggc ggaatctatg aaagaagctg ttgagaaaag cttcatgctt
    32341 gcaaaaaatg acggtgttgt gcttctttct ccggcatgtt caagttatga ctggtataag
    32401 aattataagg aaagaggtaa cgatttcaga aatagggttg ctgaattaaa aagaggtctt
    32461 gaaaagtgac aagggcgctt aaactttatt tttccatttt ctttcttctt gttgttttcg
    32521 ggatggtaat ggtgttcaac atccgtcttt tcaatgtccc gtcgatccag aatgcgctgc
    32581 tgccgcttct gaaaggtctc gcgatgaatc ttctaatcgc atctctcggc tttaccgcaa
    32641 gctatcttgt caatcttgaa gcgataaggt catggataaa atatctcctt atttttacaa
    32701 tacttatcct tatttcaaca tacataatcg gaacagagat aaacggttcg aaaagatgga
    32761 tagatctcca ttttatgatg atacagccgg ctgaatttgc taagataact gttgttcttt
    32821 atctttcaga ggtcatttgc aacaagaggg acaggatcgg gatactcaag gagctggtat
    32881 atcctttcgc tgttgtgctc atagtagttt cactcattgc atttgaagat ctcgggaccg
    32941 gagccataat attcactgtg gctattttca ttcttctcat ggcaggaatg agagttaaat
    33001 attttgctgt acttattgtg cttgctcttg ccggagccgc atttcttgta ttcatgaaac
    33061 cgtaccgtct tgacagaata aaggcgtctt tcgatccatg gcagtacaga aagaacatcg
    33121 gatatcagca ggtccaggga gaagtttctc tcggcagggg cggtgttgcc ggagtgggat
    33181 tcggaaattc ccagaaaaaa ctccgctatc tccccgaagc tcacaccgat ttcatatttg
    33241 ccatcatagg cgaagagttc ggatttatag gagtgaccat acttataata ttgtttttat
    33301 tgatgtttat aaccggcagc tattttgcgc ttacagttca caacagattc gggttctatc
    33361 ttatctccgg attcgtggta atgcttgctc tccaaaccct catcaattta ggtgttgtta
    33421 catcctgtct gccgaacaag ggtgtgcctc ttccgttcat aagttacggt ggaagcgctc
    33481 tgcttgcgta ttcgatgatg gtgggtttta ttttaaacgc tgtgacagct gataaccgtt
    33541 acagcagttg aggaggtgaa gcatggaaaa aattggaaca aggttcatta ttcttgtcgc
    33601 cggagtaaac aagaaggagc ggctgaaacc ggctcttgcc gttgctgata ttctcagaaa
    33661 atggggaata atgtcgatgt ttgcatttcc ggatggctct ccttttataa aaaccgttac
    33721 tgaaaaggga tatgaaacgg taaatgtgga caagtttcct gaaaagaagg gttttgtcgg
    33781 taattttctc agatcactca aaagaaaatc atcgcttaaa aattcaatga aaaccctttc
    33841 tacaaaacac atacaggcgg ttcttacttt aggtggcatt acatctgttc ccattatgga
    33901 tgccgctgtt aaaatgaacc tcaaaatctt catgctcgaa ccaaatgctg taatgtctga
    33961 ctgtaacaag gaattcatct cctgtgctca ccggatatat cttccttttg ccgaaatgat
    34021 agacagcgtg ccgaggtcaa aggcgcttgt tgcaggtgtt ccggtggaaa aggatgttat
    34081 gacggctgaa aagaggaaca ttccgacaaa caaaaagatt ctggtaattt tcacatgcag
    34141 aaaggattca tattcgataa atgagctcgt aagaacgatg ttcagaaagt atcctgaaat
    34201 gaggaaggag tttttcatac ttcaggaaac aggtgaaaag gatgttgcgt cgatccagag
    34261 attctatgac gaagttcaag ttgaggccct ctgttatatg cagtatgaga acaggggaaa
    34321 atattataag acggctgatg ttgtaatatc aaggcccaca gctgatgttg tgtcagagct
    34381 tctggcaatg cataaaccgg gaatattcct gcctctttct gaacattatg atgtctatca
    34441 gaaacacaat gctaaagtgg tggcaaaaaa ggagataggt tttattgtcg aggatggcgg
    34501 aagcatgcct atgagggtga aaaagcttta tactgcactt aattcatttc ttgtcggaaa
    34561 tgcaaagata aagcagaata ttgataaact tgattacgaa aaagcggcaa tgaaagttgc
    34621 tgaagatatt gaaaagaatc tatcggggaa ggtgtaacgg aatgcatgtc cattttgtcg
    34681 gaataggcgg aagcggtgtc agcggactcg ctctgatggc acaaagaatg gggtatgaag
    34741 tgagtggttg tgatgaaaat gtgagccctt attttaaaat ggtggagtcc aaagggatca
    34801 aatgttattc agggcactct tcagaccaca tgcagggagt tgatcttgtt gtaagatcaa
    34861 gtgccgttcc gatggattca gaagagataa gatatgcttt aaaaaagggg atagtggtca
    34921 tttcaagagg aacctttcta tcaaaaatga tggaaggaaa agatgttata ggtgtcgccg
    34981 gaagtcacgg aaagacatct acgacatgga tgatatatca cattttgaaa aaagccggat
    35041 tgaatccatc ggtttatgca ggcggtaaga gcggaggtgt ttcaaatatt tctgaaggat
    35101 cgccatatat aattgaactt gatgaaagcg acggatccat gtttgaaaca acccccggga
    35161 tccttttgat aaacaatctt gaatttgagc atgcagattt ttattctaat ccggaagaaa
    35221 tgcttaacag ttttgaacgg tatcttcttt cctgggagcc ggatacctta ataataggaa
    35281 gaggttatga tctgagtgat gctctttttt caatgttcac accgtcatct tttcttacag
    35341 ttgccgaatt aaaggagaac gggtctttcc agaatgtttc aggactcgat tttttcgtaa
    35401 aggaagaagc ggtatatctt ctttctgatg acacggaaat ttatgtcggt tctgtcagag
    35461 agccggcgca tatccttcag aacaggtctg ccgcccttct tgcttcactg agctttctta
    35521 aaaaaataaa tgtgaccata ccaaagatag attttaaaaa actctggact gagattccgg
    35581 tggtagacag gagattccag gtcgtaggaa aatataaggg tgttgacttg atagatgatt
    35641 atgcgcatca cccctctgag ataagagctc tggtggaaca ggcagagctt aaatatggga
    35701 atttctgtct tattttccaa ccgcacaggg tgtcaaggtt cacggttttc tatgataaat
    35761 ttcttgaagt tcttaaatat gtggaaccct tgatagttct tcctgtctac aatgcaggtg
    35821 agacggttaa aggtgctgat acggagagtt tttacagaga gctgaaacag aatggaagtg
    35881 aagtctatta ttttaaatct gttgatgatg caggtgtttt tataaagaag aacattgaag
    35941 gacttcatgt ttcagctgtt atcgctgcgg gagcaggtga cctcaacaaa gtttttgaac
    36001 ttgtgggggt gaaatgaaac tgaaaaagaa cgcactgttc ggaaaccttc tttccattag
    36061 ggtaaaagat gccgtatgcg atctttattt cccggaatcc atagacgata ttgtcagatt
    36121 tactcatgaa ttcaatgact tttatgttct ttccggtgga agcaacataa ttgccggaca
    36181 tgttatgaaa cctgttcttt acatgggtgg aatgatagga acgagcgata ctgaagatgt
    36241 cagtgaacat gcagtaaaaa cctacatgcc tgcgggggtt cccataagca tgcttataaa
    36301 ttattcaatg aagaacggtc taagcggagt tgagttcatg gcaggcattc cgggtactct
    36361 cggaggagct gtgatgggta atgcgactcc ggctgattat tcatgggatg atgttgcagg
    36421 gtcggtctat tatgtgaaga acggtgctgt atctgttctt gttcctcagt tcagttaccg
    36481 ttctcttgat aatccgcccg aaggaaattt tgtcatgctt ggcgtagatc tggtacttat
    36541 aaaggattct gttgaaaatg taaggaaaag agtgatgtac catctttcaa gaagaataaa
    36601 gataaaaggt cgcagtgcgg gttctctttt taaaaatccg gaaggggaaa aagcaggagc
    36661 acttcttgat aaagccggaa tgaagggctt ttatgtcaat gatgcgggac tcagtgaaga
    36721 ccatgccaac attgtagtta ataaaggcaa tggcgtgttc aatgatttct gtgtgctcaa
    36781 agatacagcg attgcaaaag ttaatgaaat gtttggaata acgctatgtc cggaggtgaa
    36841 attctggtat gagtaaactt aacaagaaca caaagatagc agtgatagca ggcgggatga
    36901 gcgatgaaag gagtgtcagc attgaaaccg gcggaatgat acacaaaacc ttgacggaag
    36961 gcggatatgt caacactgtt tttattgatg ctggatttga ccttgatctt aagctcaggg
    37021 aattctcgcc ggaagttgtt gtgaacggac ttcacggaaa gtatggtgag gacggaactg
    37081 ttcaggggct tcttgaaatg atgaagatcc cttacacgaa ttcaggtgtc agtgcaagtg
    37141 ccgccggaat ggataagata atctgccgtg cagttatgaa agaactgggc atcagagttg
    37201 ctgaaggaat agtgttcact gctgaaaacg accttatgcc tccgtttgat cttccagtcg
    37261 ttatcaaaga ccctgtaaac ggatcgagca gaggcgtatt cataattaaa gatatggaat
    37321 catgggaaaa aactgtttct gaactggtta aaggcaaaaa atatctctgc gaaagatttt
    37381 tcaaggggag agagataaat gttgccgttc ttatgaatga agttctgggc gatgtggaga
    37441 taattcctgc gaatgaattt tatgattttg aaagcaaata tatgagtgat ctcacaaaat
    37501 acattgttga ccctgattac aacgaaaagg tgagcagaga tatatggaga gcttcacagc
    37561 ttctgcatac tgctctgggc tgcaaagggg tgacaagaag cgatttcatt gtcagcggtg
    37621 acgactatgt catgcttgag atcaacactc ttcctggaat gacatcacat tcccttgttc
    37681 ccatgatcgc aaagaagagg ggcttaagct atttgaatct catagaactt ttaataaatg
    37741 aggcgctcgg ttgatggaaa agtttaagaa aatagcatcg acactgtttt attcatttgt
    37801 tctctatttt cttacattca tatttgtggc ttttttcaca ctttcagcac aggttctccg
    37861 tgaatggaaa caaagcgaga tggtcaaggt ttcggatgac cgcgttgaaa taagcggcaa
    37921 caacatgctt tcaagaaaag aggtgcttct catttccggt cttgagaatg aaaagagctg
    37981 gtttgatctg gatgaaagac ggatggaggc atatcttctt tcaagcggct gggtgaaaag
    38041 ggcatttgtg acaaaactct ttccggattc tgtgaatatt gtaatagagg aattcgttcc
    38101 tgcaatagtt gtcaacagca gaagatcatc attgtctcca aatgaaaaga aagatgtttc
    38161 caccctgtgg tttgccgatt ctgaagggat agtgtttaaa agaactttcc ctggtgagac
    38221 aacggcagct ctccctttct tccacataga taatgatgct gtaagtgaaa aattgagagg
    38281 cgagaagatc gttacggccg taatgatctc aaaggcatgg aacgatcaga gatcaatgtg
    38341 cagcattaaa agcattaaat ttgacatgtc atctggtttt tcagctgatt gcgaggcccc
    38401 ggattcaatg acgacatatc ttgctttcgg gaacgagttc tcaaataatg agatatctac
    38461 aatgaaagag atgttctcta atatttccgg aaaactttct tcagagaaca aatgggccgg
    38521 agagtacatt ttcgagaaaa ctgaaaaaga taaaaaaata agggttatag ttggaaaagt
    38581 attcaagaat gtgaacaggg ggagtaatgc ctaagaatga cagtgtgatc gttgctcttg
    38641 atgtgggtac aaaaaaggtt acaacgcttg tggccaaagt tgaaaatgat aaaaaagtgc
    38701 agtttatcgg caaaggtgtc gcaaagaatg aaaacggcat aattgccggc agtgttgtaa
    38761 atatggaagc gactacgagg gctattcatg aatcggtgac tgaagccgag aaaatagccg
    38821 acatccacat caaatctgtt tacatagggc tttcgggaga acatgtctcc tcaatgaata
    38881 gtcatggtgt agttacagta agagggagag aggttgcaca gacagacatt gacagagtca
    38941 ttgaacaggc aagaaatgta agtcttccaa gtgacagaca gatcataaat gtcgagatag
    39001 gtgaatttgt agttgatgat caggcgggca ttagaaatcc caaaggcatg gccggaagac
    39061 ggcttgaggt taaagtgcac attcttactt catccaccgc aatgctcagg aacctcgcaa
    39121 agtcagttga agatgcaggt cttcatgttg aagatgttct tgtcaacggt attgcaggaa
    39181 gctgggcggt tcttgaagag gacgaaaaac agcttggtgt tgcacttatc gacataggtg
    39241 acggaacaac ggatataaca gtttatcata aaggggatcc tgtttataca accgtaatac
    39301 ccatgggcgg aaggtatgtc acgacagaca tcagctatgc gatgagaatc cccccggtgg
    39361 aagctgaaag gataaagtgc agatacggaa gcgcaagacc tgattatgtt tcaagcgatg
    39421 aagaaataga agtgagcact atcggaacgg aagaaaagaa agtgaaaaag gttcatttcc
    39481 ttgcaagtgt tcttgctccg agagttgaag agatattgat ggccgcaaga aaacggctta
    39541 atgaagagat gaaagagagt cttatccaga caaacatagt tctgaccggt ggaactgcgg
    39601 aattgaagga tattcagttt ctggcaaaag atgtttttga acaaccggtc cgtatcggaa
    39661 aaccgaaaat aaacgaggcc agcagcggtt tcaaggatgt cctgagttcg ccatcctttt
    39721 cgacggcagt gggaataatc aattattttg ctgaaagagg caaaatgaag ttccgggaca
    39781 agaacggcgg atttttcagt aaactcggtg atttttttag aagatttttt gaataaagga
    39841 ggcggaaatg acggggatac tgaacagtat ggttgtcaag atcggggtag tgggtgtcgg
    39901 tggcggcggt tgcaacgctg ttgatatgat gtgtgatgaa cgagacagga taaacagcgg
    39961 agcggatggg agaaactatt ccgttttcaa caatgtcctg attattgcgg caaataccga
    40021 tgtccaggca cttgaaggaa ataagtccgc tgaaaaactt cagctcggtc cgaatctcac
    40081 taagggtcag ggggcaggag gtgttccgga gatagggaaa aaggcggcgg aagagagcaa
    40141 ggttgatata gcaactgtca ttgacgggct cgacatgctt ttcataactg ccggaatggg
    40201 tggaggaaca gggacaggtg ccgcggcggt agttgccgag gttgcaaaga attcaggcat
    40261 actcactgtc ggcatagtga caaaaccctt tgattttgaa gcaaaaagaa agcagagata
    40321 cgcaatggag ggaatggatg ctctcagaaa gcaggtcgat actcttgttg tgataccgaa
    40381 tcagcgtctg cttgatatag ccgaagacag cgatatggcg atagatatgt ttaagaaacc
    40441 caatgaagtt ctgatatatg ccgtaagaaa tctttccgag ctgataacga ataagtcgtt
    40501 cataaatgtg gatttcgccg atgttagaca gactatgacc aacatggggc tggcaatgtt
    40561 cggtttcggc agcgcgactg gagaaaatgc ggcaataaac gctgtaaagg aggccctcaa
    40621 caatcctctt cttgccgatt tcacaatatc gggatcaaag aaaatgcttg ttcatttcgg
    40681 cggagctcct acacccatga aagaatttgc aagtgcggca aaatacctga cagaccagct
    40741 gcatgaagac ggtgatgtca tctggggcgc gtctgttgat gaaaaagccg acaaggttta
    40801 cgctcttatc gttgcggaag ctacagaaga tacaataagc acgatagatc ttcccaaaag
    40861 catgaaaaaa gttgaagcaa gtgatcaggg taagttgttt gaagttaatg aaaaaccggt
    40921 cgaagttctg gaacagagat ctgattttgt agaaatggac gagatagaag ttgatgttct
    40981 tgcccctgcc gcacagaata ccggcagtag agataacgat gaagaggtct ttaacatcga
    41041 tagagagaag atttcagcca cgaatgtgtt cgatgacgag ataattgtca ctgaaagtaa
    41101 atacaaccct gaagatacta cgatcccggc gtatctgcgc taccttaacc gtaacaaaca
    41161 gaacgaacaa gtggttacgg aaaagaagct tgacagaata cagtcttcta aaaacaaata
    41221 aaccgtgaca gagaaacctc ttttagtcta tccggataga acggatattg ccgtttcaaa
    41281 cctcgctgta cactggtttt tcaacgcttt taatgaattt gagccggatc ttcttacatc
    41341 agacagcaaa gtcggaatat ttcacaaaag agatatcaac aaaactcctt ttgtgtttgt
    41401 atcactttca tacggacctg atattataaa gtttgccaag atactcagaa acagcggaat
    41461 acctttacgg aaggaagata gagagaaagg catgtttccc ttatttatct gcggaggtat
    41521 cgcggcactt ttaaatccgg agccgcttac tcttattttt gatgctgtgt tccttggtga
    41581 aggcgaatgt atgaaagatg atattgtaaa aatgttttct atggagacaa gagaaggcgt
    41641 gctggatcat atcaatgctc ttgaatatgc aatgactgat gtaaagccgg atgcaaggca
    41701 ggtgattgcc gcaggaggat ctttttttgt cagttcaaat gaaattcttc acaaactcgg
    41761 caactgtttc gggaatagaa aaatagttga aatgaacagg ggatgcacat caagatgcag
    41821 gttctgtgcc gcaagttatg cttacaggac tttcagagaa cctgacggaa aaaagctcat
    41881 ggattttgtc aaaaaaactg cggaacggaa agaaggactc gctctgatgg ggacatctct
    41941 tgccgatgta agcagttttg acgaaattct tgaaacatgt gcggcatccg gttgcacggt
    42001 ttctctatcg tcacttaaaa taactgaaat aaatgaaaaa agagcccttc tcctgaaaaa
    42061 atgcggtgtc aggaccgtta ctgttgcagt ggaaagtgca gatcctgaca caagacaaaa
    42121 gatattgaag aacataacag atactgacat tttcaatgcg atggagatat tgaaaaaaca
    42181 caatttgaag gcaaaaattt atttgatagc cggctttccg gaaacagatc tgcaaaaaga
    42241 agcggaatcg gtcatcgaac ttcttaaaga acttgataag cggaatcttc ttcatgatgt
    42301 gacaatttct gttgctccgc tttccccgaa accgttgaca cctttgcagg atgctaagtt
    42361 catggataaa aaacaatata agaactatat ggttgcgttg aaaaagggtt gttcagtttt
    42421 cgggaaaaga ataaaactcg attttttctc ataccgtgaa agtgaaatag attataagac
    42481 aggttttctt aaaggggccg atcttataga tctgataaat gaaaacattg aaaagggcga
    42541 tgtgtaatgg cagaaaagat tatcgttttt tgcgaaaatg accgcttagc cagatattgt
    42601 aaagtaacat ttgaactgtt atctgtggcg cgatctctgg cagagaacag aggtttttct
    42661 gtgattgcag tttcattcaa aggtcactca gaagggagcg atgcactttt tgcttccgga
    42721 gccgatgagg tcattgagcc acactggact gaaaaagagg gacttttcaa tcttgaaacg
    42781 gttactcata ttgcttctga agttatcaga aaacttgatc cggtgatagt tctattcggt
    42841 gcgacagatc ttggcagggt acttgctccg aaagttgccg caaatgtgag atgcggaata
    42901 acagcagact gcaccggttt tgaaataaac aaagacggta aactggttca gataagaccg
    42961 gctctgggtg gaaacatact tgcccacatc atatcgcccg aaagactgcc gcagatggct
    43021 actgtaaggc cgggtgtttt caaagtgaag gagttcttta atcatgaaag accgatcgaa
    43081 aagatgttct ttgaaatacc gaatacaagg cggcatgtta ttgaaaacag cagagtgtca
    43141 aatatgtcgg cagaatgtga aaagatagag gacgcaaaga tcattgttgc tgtgggacac
    43201 ggtgtaagat cgaaagaact gcttaaaaaa attgaagatt ttgcaaaaag catcggaggc
    43261 gtctgtgcgt gttcaagaaa aattgttgaa tcgggctgga tggaacacag tagacaggtg
    43321 gggcagtctg gaaagacaat ttcgcctgat atttatatcg cgatcggaat aagcggtgcc
    43381 gtccagcatc ttgcagggat gaagacttca atgaagataa ttgctgtaaa tactgacgca
    43441 aatgcagaaa tattttctgt tgcccataca gggtttgtca tggatgcttc catctggctt
    43501 gaaaaagcga cagaattttt tacaaaaaag gtttaacatg gacagattga aaaatatttt
    43561 cagtagagat attgaaccgt ctgacagata tctggctgat gaaagcagaa tgcagggcgg
    43621 agatgcttca aaagtccttt ttccggaaaa cagcgtggaa gtggctgaaa tacttaaata
    43681 ttcttcatta aataaaattc cggtcacgat atatgctcaa ggaaccggca ttaccggagc
    43741 gtcagttcca ttcggaggga tagtgatctc tcttgaaaga atgaataaaa tcggcgagat
    43801 agagcttgat gctgaatcgg gagagcactt tgtaaaagcg gcagcatgtg caactcttaa
    43861 cggaattaag gatatattaa acggcacaga gttattttat cccgtagatc cgactgaaat
    43921 gagtgcttcg atagggggaa cagtcgcaac aaacgccagt ggtgcgcttt ccttcagata
    43981 cggtccgaca agaaaatgga tcagatctat cgaaatcgtg ctgatggatg gatcaattca
    44041 caaaattgtc agaggtcaaa attttgcaga tgattcaggg tcattcaatg ttttcggaaa
    44101 gagtttcagg attcctgaaa tcaagatgcc gaagtgcaaa aatgcggcag gtcttttcag
    44161 cgagaatgcg atggatatta ttgatctttt catcggttct gaaggaattc tcggtgtaat
    44221 tacagaagtt gagatagggc ttgctccaaa acacgcttct ataagtgttg taaagttttt
    44281 tgatactgaa attgaggcaa ttgattttac ggcatatctt aaagacagca aagatgtaat
    44341 gcctgatttt attgagtatt ttaatggttc gggtcttgaa atgataagag aaaaagccaa
    44401 aaaagatcct cagtcagtca gcatgcccaa gataagtgac aatgcgaacg cggcactgtt
    44461 ttttgatatc gaaattaaag ataacaacat aaatgccgcc tttaacaaac tgctctcttt
    44521 aaatgttgac tggaaaggtt catggtgtgc atgggaaaat attgaaaagg aaaggataaa
    44581 aaatttcaga catgcacttc ctgaaacagt gaatgaatat attgccggcg ttaaaaagaa
    44641 acatccgtcc atacataagc tcgggaccga ttttgcggtc agtgacgaaa aattcggaga
    44701 gctggttgaa ttttatgatt cagtcctgaa tagaagcggt cttaaatatg tcagtttcgg
    44761 ccatatcgga gataaccatc tccatattaa ttttctcccg tcaaattatg atgaactgga
    44821 aaaagggaag gaaatataca aggttatcgc tgaaaaagtg atagaactga acggaagtgt
    44881 ttcagcagaa cacggaatag gcaaactgaa gcacagatat cttgaaatga tgtacggagc
    44941 tgagggtatt agagaaatga gaaggataaa aagtgttttt gacccccatt ttcttctaaa
    45001 cagggggaac atgttccctt gcggggaatt atgatgaata ttgcagttct cataaaacag
    45061 gtgccccgta ccgataagat aaagatcgac agggaaacgg ggactttaat cagaaaaggt
    45121 gttgaatcga tac
//
LOCUS       DAOOYB010000002        44151 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_709_length_44151_cov_10.140829, whole genome shotgun sequence.
ACCESSION   DAOOYB010000002 DAOOYB010000000
VERSION     DAOOYB010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 44151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 44151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44151
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_709_length_44151_cov_10.140829"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            119..427
                     /locus_tag="PL056_00220"
     CDS             119..427
                     /locus_tag="PL056_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45467.1"
                     /translation="MKKFARISVFLSAIVLIFMFLDFMLAKRAKEIISDVQSRKTEKI
                     IIFNDQPGDICNALTGGIWLKPVMIFTNKLEMPSNCKVSVTGGYCSRTKKHQIVFTCK
                     "
     gene            611..2611
                     /locus_tag="PL056_00225"
     CDS             611..2611
                     /locus_tag="PL056_00225"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006513876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HPM45468.1"
                     /translation="MRYFFSLLAIAVLAISCSNNQNIKEGKSMVKAEGAKPLSYLSEL
                     GEMIPREVLFGNPDKTGVKISPDGLKMSWLESDESGILNVFIKNYPEGELKQVTKDEK
                     RGIRSYGWTYDSEKILYIQDKDGDENWHLYIVDLKTGETKDMTPFPGVRAQNLILDRK
                     FPDKALIGLNKRDAAVFDMYRLDLKTGELEMTAENPGDVAGWGTDHEFKLRIAVATDP
                     VTADEIVRIRNNETEPFRELIRIPFGENGGVINFAPDNKNLYFLTSTGSDTVRLVLLD
                     GENGAELKTIFVNEKADISDVMTDPDTHEILAVSVEYTKLEYFVMDESVKADFEYLSE
                     LKDGQFYIVSQDLSKTKLIVAYMLDNAPYSYYFYDRSSKKADFLFTHNQDLEKYKLAK
                     MDPVIIKSRDGLDLVSYLTLPLNVKPENLPLVLDVHGGPWARDSWGYNSVHQWLANRG
                     YAVLSVNFRSSTGFGKKFLNAGDNQWGKNMQNDLTDAVKWAIEKGIADKSKVVIMGGS
                     YGGYATLAGLTFTPELYAAGVDIVGPSNLKTLLETIPPYWATFKKQMVLRIGDVENNE
                     ELNKEISPLFHASKIKAPLMIGQGANDPRVKIAESDQIVAEMRKNDLPVTYIVYPDEG
                     HGFARPENRMDFFGRVEGFLSFHIGGRAEKFEKDDKSSALEK"
     gene            2622..3194
                     /locus_tag="PL056_00230"
     CDS             2622..3194
                     /locus_tag="PL056_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005785730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HPM45469.1"
                     /translation="MALSYPYIFLLALSLAMDAFAVSISAGISMQKRDFSEALKIALA
                     FGIFQAVMPIIGFFGASSFYDYICHVDHWIAFFLLAFIGGKMISEAVKKKGECEVFPA
                     NIKFLQLMVLAVATSIDALAAGVSLSMLCSDILIPVLMIGFVTFLFSFSGVTFGAKLG
                     CRFGTRMEIVGGTVLILIGLKVLLGDIAGI"
     gene            complement(3191..5695)
                     /locus_tag="PL056_00235"
     CDS             complement(3191..5695)
                     /locus_tag="PL056_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45470.1"
                     /translation="MSVFFNGIMILTTVFFLIFTGCDNGRESKNDQNSELSDDFKDSD
                     DLFQTDSDGTFEQEGNSENEEMAENEETPDTDSETDNENSDSDIDATLCNPNPCTEKN
                     KNVCIELYDVFYNFDLAAKCFCNDGWIGKLCNRCQKGHFGKNCKPCECKKNEVCNDGL
                     DGDGKCSCATEIVEGKCKCYPGWSGEECELFGEKWGCVAIGEPCKDSEECCFDGLCME
                     SLLQEGSFCRKTQMYGSEEIRRFAVSPDSSLIATARMGAIRLHRTDNFKEIFRVSFSP
                     STAIAFSPDGKFLAADEWDTLVLRSMEDGTILDSAYHSYSQMIKFSPDGTMLAVGDSD
                     GFTVYDVTSDSLSHYFSDTPFQTGYGWNVESIDFSKDSRYLVAGSGLSSFYENTMIYD
                     LKEKEVLKILPVDEYYVEVAFSSSNIIATGGYPREYDDNVNVFKYIDSEWKFSHSLQG
                     PSDEIGSLSFNASSDFLAVGGRQYSFIYPLYDRFYPILTDGWQMEFAPDDSALVIGSE
                     TGELQKYSLPSFNKYFSSRYNGSNNLKISSDESEAVFLRGNQVIFIKPLTGEVIKEAT
                     LPFDPFYYIFAFSDDLKMAVFLLDYYNDEGFALYNFTDNIVVFEKSPQYYYFEFSPDD
                     KILAAWSGYDINDVSFYSILTGEELHKISFDDSDKVSAVKFSPDGKIAAVTHKNGNKI
                     YDTAEWNEIFSLPFGNVSYFPDGSKIIVTNGDIKLYSSDDGSEITEPQFNINTKTAIL
                     SSDEKYIVTDSAIYEISSGETVPFETHYYGISSTGISSGNSILTTSGTNTLRVIDAKD
                     ILDKYEGDCSENSDCEPGFFCSKRVKRCFANIEYEP"
     gene            complement(5731..8214)
                     /locus_tag="PL056_00240"
     CDS             complement(5731..8214)
                     /locus_tag="PL056_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45471.1"
                     /translation="MFTSRRIFLFIIPFVLFAGCESSKVGENDENDTDIYDNDFVENE
                     KYEENQQDADEADNEYNESEQPDETADIYSETDDEDSDADAVKNLCDPNPCNFKNKSV
                     CIEIDIWRTDKRRYYCSCDKGWFGPKCDDCQRGYFGNNCKPCDCKKNEVCNDGLEGDG
                     KCSCATEIIDENCACYPGWSGDDCEIFGENWICAPEGENCDGGVDCCFDSVCILSKCV
                     KARRYDSESALLADSQQGTLIAAVRSGFIYFYDIDTLKEINILGEGRTANIDFSPDGS
                     LLAAVNDTCLYLYNLDNGEKEAVGCGLNYCQELKFSPDGTMLAATNWGGFDVYMLNNG
                     KFEPYFSDSFENIAIGINTYSVQFSADSSLVAAGAGASMDYDNIHIYDLKEKKLIHEL
                     PLYEYYAKLSFSEDGRYIAAGGYSLDGAIDGWFEDPAYIYNCSDWSLKEHINYTLDSL
                     SSLSFNYSGDYLLVDNVVYRVGGHFFPFVSNHSSIIFSKNGQNLISIGEGALHKYSIP
                     GFEFLESGTKGGGRAHFFSHDGKSVVSFTSSYETLFSDPLTGNELKRFKMEDLNQFYL
                     NFKLSDSGAYSISYADDYYRRKGFFITDLETGKIIYNEDEIMARAAAFSTDDKIFAVH
                     DGITFTTHLRLINSGDLIFSIQHNEEDLVSDLVFSPDGLLLAVGFEERTTLYDMKTGL
                     EIYSVPFSGHTSFFPDGSRFMIIDLMPNVFSTVDGNAVSTSFSGIISEQAVISNDQKY
                     IIFDRGIFDISTSTLHSFDKIDFDIDKIALSPDSTLLFLSGTYFARVVDTSNSLEKFE
                     VECQNDLDCDTGFFCNTASGRCFAQTGQY"
     gene            8388..8744
                     /gene="yajC"
                     /locus_tag="PL056_00245"
     CDS             8388..8744
                     /gene="yajC"
                     /locus_tag="PL056_00245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HPM45472.1"
                     /translation="MNANVLKLLGDLATGQAAGAGGGAAGILNMLLPFILIFGIFYFL
                     VILPQKKQQKKHVEMLNQLQKGDMVLMNCGIKGRISEIRNNDIKLEISPKVIITIQKG
                     VISGRITATEEEEKEN"
     gene            8767..10500
                     /gene="secD"
                     /locus_tag="PL056_00250"
     CDS             8767..10500
                     /gene="secD"
                     /locus_tag="PL056_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006424575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HPM45473.1"
                     /translation="MERNWLARAIFTLALIIVAVVMLIPTFVDQEKNPWVSKINDGIS
                     LGLDLKGGIHFVLSVDTEKAVQDRLDRRTDEIRMRLEEESLPFESVTVEPENYSIIIK
                     ITENTDKDKFRDKIIDFFNDFRKTSTDGNIHTMKMRDDMIAQIKESSVEQALETLRSR
                     IDELGLKEPIVARQGENAILIQLPGYKDVERARSIIGQTAQLEFKIVAEDMDPLGEYT
                     GEVPEGITLVQGRGTNSDGSVLTYKYATGKDRVAMRAFLKDKAPEGTEFLLEESVFPN
                     GEKEYQSYLLYRQAYITGDMLTDARVSIDEQKNRPYVSMTFDKNGARIFAEVTGKYVK
                     RKMAIVLDEKVNSAPVIQSKIEGGSAMITLNSMGDYNTVFEEAKDLALVLRAGSLPAP
                     VTFEENRTVGPSLGHDSIEAGKLSVIIGFLAVVIFMIIYYGLAGIVANTALFLNILFI
                     MAGMAAFGATLTFPGIAGIVLTIGMAVDANVIIFERIKEELRLGRSLADAISTGYDKA
                     WSAILDANVTTAISAFILWEFGTGPIKGFAITLLIGILSSMFTAIYVTRVIFNYMMKA
                     GLKRFSYRDKN"
     gene            10516..11637
                     /gene="secF"
                     /locus_tag="PL056_00255"
     CDS             10516..11637
                     /gene="secF"
                     /locus_tag="PL056_00255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HPM45474.1"
                     /translation="MTISNRNIDFIGKFKLFGTISAVAVTLSLIVFFAMGLNYGVDFA
                     GGTEIQIKVDGMNNQELRTIASEFGSVDIQEFEGRDNEFLLRFLNISTVDDATINSFV
                     EEVKNEFSSSKMTKKHFDSQVGDRVEVWFENEIDMEKLSSMGLNHKIPATGKIEYKKV
                     GERHIYRIMLEGLTTKILSSIKEKSGKEPELMRVELVGPKVGEKLRFSAFQAVLYSLI
                     AILIYIAVRFNFQFAPGAVISLAHDVMITLGIFSVFRIPFDLTIVAALLTIVGYSLND
                     TIVIYDRIRENWNNPKKGASVVEKINTSINETLSRTLLTSITTLIVLVALLIFGGGTI
                     SGFALAMTMGVVVGTYSSIFIASPVTVLIEKYMGKEKTA"
     gene            11638..13371
                     /gene="recJ"
                     /locus_tag="PL056_00260"
     CDS             11638..13371
                     /gene="recJ"
                     /locus_tag="PL056_00260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HPM45475.1"
                     /translation="MKEKWQILRPDRQKVDNLSKTLGISKTVATVLVNRKIDGVESAS
                     LFLQSKIKSLPDPFLLKDSEQAAVIITEYIKQKKKIVIYGDYDVDGITSTLIMYQFLT
                     HVGARVDHYIPHRLEDGYGLNTYTLEEIATNGGELVITVDCGITSVDEVKYAKDLGLE
                     TIIIDHHHVGEEIPPAVAIVNPHQHDCPYPFKEMAAVGVAFSFLMVLKKVVEKDGFFK
                     GALPNLAEYLDIVAMGTVADMVPLLDSNRIFVKHGLTQMQKNKRPGLLALANVCGLKN
                     LKEITPAVISYKMAPRLNAAGRIGNAIKGLELIECTDYESAYKMAEELNLTNEYRQQL
                     EAEIFEQAMSKVENSDIIDTKSAIILYSEKWHPGVIGIVAYRLVEKYYKPTIMISVED
                     GIGRGSARSIPQLHIYNILKELSSYLVQYGGHKYAAGLTIKESNIEEFIERFNGMVDT
                     ALAQETYSQSTDIDAELPLKELTPDLIVALSKLEPFGMNNQEPNFLATNVQIIKQSVI
                     NRSHLHWKLGVADSTMTFNSIGFGLLNEKDSPNTGDLIDIIYFPRINTYGGNITLQLY
                     IKDFVFSEKKD"
     gene            complement(13372..16359)
                     /locus_tag="PL056_00265"
     CDS             complement(13372..16359)
                     /locus_tag="PL056_00265"
                     /inference="COORDINATES: protein motif:HMM:TIGR01451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WAP domain-containing protein"
                     /protein_id="HPM45476.1"
                     /translation="MKRIFMVMIALFPFFLSAVIGPNVSQTNGQELKWEEGAWDYFVM
                     FKSLIAQVTGEVTTTPGNPQADSCIDINVGSTFTLTSAHVPPDTNIDRAFLIWIAAQD
                     PSQYNGPTDNSVTLTFTNESKPSEVTLSTEVTSSVSGNLFTPPSFDYEAISEPSDSTG
                     IFTYRVDVTEFMQQIIELGALNDITQTGEALYGSYNVKGMNCTRAQAYLTTSGMVGAW
                     ALVFVYTSERISPKKIYFYNGLEAYRFQEAYIDVSGFELPNEAEVRMTMVLAEGDPGL
                     ASATTDTFQPAPPEALAISGQSNPNFDLLWNNCNPAKYTPLAYTEVYNSISSIFGWED
                     EMPVCVGGDPNSPDPTKLEYAIDADTFLLKAKEFPFDQHLKKGDTMFSLKIGANQDQV
                     YTNLLVVSVDTKAPKFDIPANPDTPDGREKNYCSCSTEPDAVCFDRPFYYLIKVQNWG
                     ENLAENVTVQDTLPSNVEYEKGTTEIATVFKDGLGTDWKAVDDVDGNFPFATAKQVSD
                     LMGYCDKTTMDCPDTVMIRFVVRPKAGLPKHETIRNSAIITDAGKLPYSTNSNIALRL
                     KNGTCPAVTECSLPPKAQCGGVGQGTDDYCEEDKDCKDGKKCIDNQCVEEPKDKTKDA
                     QVSFGEGLNSPDSSTTIIIPSPKKDLVVGQFYLISEGDTGKTYDLEGVAVRFTYDSDV
                     KISNIRLIADDNGNGAYSDSKTELAKTNALTGDNYAQFDIIDTGKRAVASGIKNNFII
                     VADVSTNITSGIAGSFYAEIQNKEAIRISDSGEPVVSGEGFEFATFRFEPPEGFIFTK
                     GENDPQVPSFKEMNKDNPVLQVRTKSMEGGDAIKSIFIKSSTGFARFGEGIESVTLIL
                     DDNGNGEHDPEDTLIQKITEFENPTSFTISSLDSILSYSKDEQKYLLFKCGFKMKEGD
                     KAKITISKVSLTEDREIAELPVSSKEFKYLCDDADPNTCVTDEPGKKGGCSLTVVEET
                     EGPLAVIIAMVFGMFLVFRTRED"
     gene            complement(16553..17299)
                     /locus_tag="PL056_00270"
     CDS             complement(16553..17299)
                     /locus_tag="PL056_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005651222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HPM45477.1"
                     /translation="MKTVYDLLLAAQFISAAFVFIALYFVTAGYGRHGGKKWGPSINP
                     RIGWMVMESPVVFIPLYFLFTGKPEAVPVVMLTIWMVHYLQRTFIYPFLIKGEENMPV
                     LIMIFSIMFNSMNGFLNGYYLFHLADYDLSWFFDPRFIIGTVIFFSGMVINIHSDHVV
                     RALRAEKGPGYHLPEKGMHKFVASPNYLGEIMEWTGYAILTWSLPGLAFMVFTMANLV
                     PRAHKHRKWYRETFGEKYPSTRKRVFPFIY"
     gene            complement(17309..18052)
                     /locus_tag="PL056_00275"
     CDS             complement(17309..18052)
                     /locus_tag="PL056_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45478.1"
                     /translation="MKYLFSGFLFVIIFFVFPLIHATENVTEGMVITPELSKKVIDAI
                     TEKYSKNMYEKYAGVKSTRHVTVEWRHPKTNELIETEKVVYVRWDDFYEDLKYEVLEY
                     TVNGEKKDPSKYDPRESKPGIPHFDKNGEKEYIRELAAVEKVKGKLCYKIKATPRKPK
                     DEHFKGFLWVTVDNLELIQNEGTSGKSRFGVSDLYVKYQAKDFGDYFHFTSGYTKVNL
                     NVLGMYKRILIFNFENKNIEPMPLPRKKK"
     gene            18216..22874
                     /locus_tag="PL056_00280"
     CDS             18216..22874
                     /locus_tag="PL056_00280"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPM45479.1"
                     /translation="MKLEKGTTLLERFELTRKLGNTAFGDVWLAEDAKKGSQVTLKII
                     SSKYFNKKTDLKKLKNEFRSFSQIKCKHISEVYEIFDEKGLLFYCAEYVEGSKISEFR
                     GEHPSVLIPFFMDISKALLELHKYGIVHGGIKPGDIIIKSDRSVKLVDIGVLSSFENR
                     NPQIRKVFNYTAPEIINGKAFDWRADLYSLGVIMYEILYTKLPWVAPPFLDNSEEISI
                     EISFPKNSSPQINGVIKKLLSIEPDSRFKDASELISSLEHIFAGTAKKAGHSANVKGV
                     LKIKETEFISRRIEMTTLMEMLDNFEATSIDSSVIIESPGGAGRTRFLREFEKRISSR
                     NLNALFFSASQSQNLATDIVDTIWESLDREYRLQLSLKWRGTVLLYFPHFKSYGEFKN
                     CPDSKHLPLANEDFYRLASLVRDFIKMGTRNRPIVMLLDNFDHVDKRSLRLIQEISAG
                     FDNSIRLFTIVTLDPASGSNIEIPAFSRITLSPFTFSETRDFIEDSLDQPANQIDNEL
                     FLWLYRNSKGLAKQIRSLLFLLIEEKVIYSSGNHIFFNYTDMGEKGIDLLLQNKINSL
                     SQNEQLTLKACSIFKKFGTKEALEYLIKDLMTVEELESAIRILEANYLIVVNKNGKIT
                     IVNRQFRPIIYETLEETEKKKLHKKMGDYISLTWQDLLSMNINHFAFAAHHYNQAGAF
                     EKALKLYLFSVGGSMAQFNSEFAESSIDDALEIVTLNPGIVKGKKLFAIYLFAGRMYY
                     KLGIYKKAVPLLEKSYEVWKEDTILEDLVFSLANDSDPSKAVKFISSYGDETPDKKAV
                     KHYLRSFVYLTTENNYTKSHYHLKRAVGYIEKGYGKLFGPIRKFTLKQLQFDLDLFMN
                     TSGFETLDKLRIELIESSKNIESKAFLIDAMNASFLFYWNYNEMKKAYNILHESLKLS
                     LEIFDNYRITRSYLNLANCSHRLGRLNDVRFYLDKAIEYARKGSGASILKQCYCNYGE
                     LAIIKGEFSLAENYLYNAEEISEREYRDPDLIIIYSLQTILSLLKNEIGTARNTGGKL
                     RRYIEQFRTVGQNKQISYFSALMLLEAMTGNDHEFFFELDNKFQKLLNSYPLFKKSNE
                     LLHITCRIIFFKRNNDRDSAINQIHEVEIKGIKSTHSLYQMIYYYHASLLLKEFSPFS
                     GLLKKYIGEGVKFAMQIQSNHFVSLFNSISYVLETDETENIINRIKDVFLDIHADEES
                     KNIIEGEIAKLHSHIQNSKNQIEHLSTMNSSYSTIIDIVKSIAGRTDFSRITETVVKK
                     MIDALSLEICGIVFLGGDSEENSYLILDSVYNEHKMSDFRFKAGVVPRMLKTGRIDFI
                     SGTPSEKNFEYRSVAAVPITLKGVIKSYIYIERDSALGTFRESEIRFLETLAESIAVI
                     FDNIELMQIATTDNLTRLYSRRHFMNILSKEVEKAGRYNFTTSMIMLDIDHFKQVNDN
                     YGHLSGDSILRTLGELLKRTVRSSDYVGRFGGEEFVILLTGTDLEGAFQTAEKIRKAC
                     EKQNVSGISFTVSLGVASFHEDRVKNEKDFIEKCDMALYRAKNLGRNRTVRYCDLLGR
                     "
     gene            22877..24046
                     /locus_tag="PL056_00285"
     CDS             22877..24046
                     /locus_tag="PL056_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45480.1"
                     /translation="MVLNIKIFEEQIIQGDTSHIRKEMDALLQSSAMIKKHEDDIFLN
                     FQGLTPYDIFKKLKNEQYRTWFKKLDTEVPGLPFFLNESSNSLLFFLMGNISFEEKNS
                     SVLFDKIEASRFFKEKMIQMRNLCVPHNINPQTGITKLSKVLSGETKNSDSGQNSDRQ
                     SENSSGRSMENEKKKGAIKDFLDRYGSIAFLNSDRTVKLTIVINEIPSRISFIRNFLV
                     KDPALPQQFFLTVLKTDSGISEIKSLLLASINDIESSIQHHKGVFIQVVTKSEDGSYQ
                     MLLESETIFPITVTSDLKNDIAAIESEVSVSFGTEISKTEAPDPIEKTTVPAEQKTMI
                     EKDAEPVEETEIIRLKKENEALLVKVEKLEKLIEVYEEEINKKRSIKGFFGKFFS"
     gene            24046..24804
                     /locus_tag="PL056_00290"
     CDS             24046..24804
                     /locus_tag="PL056_00290"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPM45481.1"
                     /translation="MKIFFNGTGSSPGVPQLFCDCRVCKSSFRENKRTRFSMTLIEKE
                     TIFQVDAPFEVRLQLLNAGIKKIDGLWLTHAHSDHIAGIDDLRIASFRNGAPLPLFAG
                     QETIESVKTRFPYLFFENEYTDIPLLEPVAVDEKPFQFKDMEFIPVRHFHGETKVHSF
                     RMKDFGLLADISSISESELAKMKGVKVLAVCTTVNHPHRKHMDLDEIIELIKIISPKK
                     AYLTHMSHNFDYFEIKNHLPENIFPAFDGLRVEV"
     gene            24879..25679
                     /locus_tag="PL056_00295"
     CDS             24879..25679
                     /locus_tag="PL056_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45482.1"
                     /translation="MFRISVIITVAVMIFSCSTAEKKTSGSVESGKTSSAGKHELTAK
                     GKKLEFDAKTYVIKKYDINKDKKPDIINVFRKLPIEGKRGEYDLKIHVKMMDLNRDTK
                     IDVWRFFNDSGAVIKEELDLDFDGKIDAVDHYLNGIVRRREVDFQFDEGTDIWKHFDE
                     KGALMLIEADQSGDGKIDYWEHYTNGAIERIEKDTDDDGKPDIFKRSGDADFTRILST
                     TDKFEDQNIQKIEEKPEDELPEQVEEQKEEKDASQEEPETAPRTGQDK"
     gene            25760..27031
                     /gene="purD"
                     /locus_tag="PL056_00300"
     CDS             25760..27031
                     /gene="purD"
                     /locus_tag="PL056_00300"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013451202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HPM45483.1"
                     /translation="MKVLVVGKGAREHALAWKISKSPLVKKVYAFPGNAGIAQVAEIP
                     DIKSDDIIDLASFALKEKIDLTVVGPEDYLAKGVVDVFQFNGLKIFGANKAAAALESS
                     KAFAKEIMDSAKIPTAAYTTVSDFEKGATVLKTSKYPVVLKADGLAAGKGVVICDSYE
                     QAAKELSEMLSGKFKDAGTKVVIEEFLKGEELSLLLLTDGTNVRKLLFSQDHKALLDG
                     DKGPNTGGMGAYTPVSFADDLLYEKVNRLVTLPLLTELKKRDITYRGVLYIGLMIVNG
                     EPFVLEYNARFGDPETEPLMFMLKSDIVPYFMACAQGDLEKLHELEWFEGYGATVVMA
                     SGGYPESYKKGEPIQGLDVIEENAVVFHAGTTMKNGNIVTDGGRVLMVTASGKTIHEA
                     MKNVYSNTEKISFNNSFFRHDIGHREINRNK"
     gene            27031..27597
                     /gene="yihA"
                     /locus_tag="PL056_00305"
     CDS             27031..27597
                     /gene="yihA"
                     /locus_tag="PL056_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009433530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HPM45484.1"
                     /translation="MIIKTVSFIKSASSVQNLPSTGLPEFAFIGRSNVGKSSLMNMLM
                     GRKNLVKTSKKPGKTALLNYFIINDLLYFVDLPGYGFASRSKSELDSWRSMIEEYLLK
                     RTELLEIFLLIDSRHGIMANDEQMMTFLEHHRLNYTPVFTKTDKINKTELNVLKQKNR
                     DSFFCSAVKGDGRELLLDHIEKIIGKKE"
     gene            27599..28126
                     /locus_tag="PL056_00310"
     CDS             27599..28126
                     /locus_tag="PL056_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45485.1"
                     /translation="MKLFILIIIMFFTFICHPSKVIVESVVANMNDRAITYSEILQEG
                     ELLNIESNVDPRTPLSAELKERILELIMFRIIVFEEARSEGVTIDESKIQEKVKTYLS
                     NVYMKDFRKTYSISDLEFNTMIKIRLTADKMTEIFLDKKFKGKTPSKEEKQKAVTEWQ
                     KNLLKKQKLVLYSIP"
     gene            28139..28903
                     /locus_tag="PL056_00315"
     CDS             28139..28903
                     /locus_tag="PL056_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005838337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HPM45486.1"
                     /translation="MGKVISIVNQKGGVGKTTTAVNLAACLGVMEKKVLLIDMDPQGN
                     ATSMSSVKGENFNTIYDMMADPSVNAIYPTGLPFLKIIPCNSELTGAEVELLQMDERE
                     FVLRQTIDKLRNKFDFIIIDSPPSLNILTVNNMVASDGVIIPIQCEYFALEGLSRIIE
                     TIKTIKNLNSDLEIYGIVLTMYDKRVKLTYEVENDVREHFRETVFNTVIPRNVKLSES
                     PSFGMPIVLYDISSIGAKKYLELAEEFLSRFKGGKK"
     gene            28900..29724
                     /locus_tag="PL056_00320"
     CDS             28900..29724
                     /locus_tag="PL056_00320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HPM45487.1"
                     /translation="MIEKRKLGKGLNALIKSSPENDYMVLSIDSIITNKDQPRKRFDE
                     QRLTELAASIKQYGIIQPIVVKKEGAKYRIVAGERRYRAAKMAGLTEIKAILFKGEED
                     YQISLIENLQREDLNPIEVAEAYTELIKRFSYTQQELSEKVGKSRSEISNCMRLLNLT
                     GKIQDLVRNGTISVGQARPLAVLEKSEQEAVLDKILNKKLTARQVEKIASSKQNKTAE
                     KNASGGYWELQQKELAVHTKAKVSLKEKKTGISVTFDFGTISDFEKFSAIVRKLNK"
     gene            29721..30404
                     /locus_tag="PL056_00325"
     CDS             29721..30404
                     /locus_tag="PL056_00325"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HPM45488.1"
                     /translation="MRSLLFFLIIAVTSSIQAQKKFTVVIDAGHGGSNKGAISGEIEE
                     KELTLELALKLKAFFDKHGSENIHAVFTRTNDTELPVKNRIELIERIKPDLFISLHFN
                     SQKFLSTNRGFEVYYPADVINEKSVETAQKYDRANRSFIYGSIFKEMFFKANLHTTWK
                     LPFNLFTQKYDLMMFDDTTAPGLLLEIAYLTSPEDRACIENPDFVADIAWYIYDAIVK
                     IAEKSRRGK"
     gene            30401..31783
                     /locus_tag="PL056_00330"
     CDS             30401..31783
                     /locus_tag="PL056_00330"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPM45489.1"
                     /translation="MKKQWKILIVDDDLSISRSYQKYLESEGYIAITANNGREGLQKI
                     EKEDPDLVLLDINMPVKNGLELLEDLNRTKEIKMPLIIMLTAYGDLEAAIRSTRLGAY
                     DFLTKPVSLEKLRLTVKRGIEKIALSEHVAHIIEQDHDGFKDTIIGRDSRMIDIYKII
                     GSLESNKATVLITGESGTGKDVVAKAIHENTSKDEPFIAINCAAVPENLIESELMGYV
                     KGAFTGAISNREGKLEAVKKGTLVLDEISETPLEFQAKLLRLLQEKEFYPVGGTEKKR
                     FNGRIIALTNKNLKDLVEKGKFREDLFYRLNVINIVVPPLREHMNDLEMLVIHFMKKT
                     NYLMHTAVESITREAIDFLMTHVWPGNIRELENVITKAAINAKDRILTKDSFGFLGSF
                     NEIDGSNDKLRINSDNEELLPLKDIEKKHIEFVLKQTNWHKGKTAEILGITRPTLDKR
                     IEEFGLAKDV"
     gene            31776..33752
                     /locus_tag="PL056_00335"
     CDS             31776..33752
                     /locus_tag="PL056_00335"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM45490.1"
                     /translation="MSETGFFLPVFLFFAIVISSLGWQILIKGVGVVDNLWAFGLLFS
                     TVFMLTASIVTYFPFSGNAFFISILIFMNLTTLYVYSFPKSIWPNMIWFLFIITADFL
                     SSVAFLWSRENCHLAYFLMPALFSSINIPIIVRKVKHLEKVSAKAVLIHVSFISFLAI
                     LFPIAAGISSMNFNPLLFLILLTFTVPVTLVLFGISSYLNINTGSGISSINFPFIIFL
                     SIPGTVIIKNMFSLRVTMLNYIDSVHFTFGALLLLFAAVSIAGILISMFSTSIEGFIN
                     RNRHFYQKYLTSYRTVIEEVTDPAELFILFDQNIASWFKEFKSVRYIFFNDEFSAGRD
                     ISFYEEPSSFNIETTDKKFLHEPYFIKNSVDVPDEITKISSKYKGNIFIPIVYRNEVN
                     GFIAIDSRKFSHSATVCVHNILEMTMNRFEKLSLFTSVIEAEKKIEMLKHFQETGKMV
                     SIIAHELRSPLSSIMFNMEVIKDSVYKKRDADPEYLDISLREIRRLNETVEKMLNYGR
                     TIKLTPSEGEFKSFFTDIDRLFPSTPEKITFIDNTNGERFYFDWDMLKSVMINMISNS
                     LQAIEGSNGIGNITVKCFKRRNRIIIEISDTGPGIPKEIRNSIFEPFFTTRKEGNGLG
                     LATSEKIIKLSGGSVILKETSEKGTVFQIVLPVE"
     gene            complement(33754..34950)
                     /locus_tag="PL056_00340"
     CDS             complement(33754..34950)
                     /locus_tag="PL056_00340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HPM45491.1"
                     /translation="MSLKFVSVGESGQNLHKIEFEGGKVLYLIGTAHVSGESVQLVEN
                     TINEYSPDTVAVELDENRLEVLKNRKRYEETDIFEIFRKGKVLFFSVQLMLTSYQKKI
                     AEKTGVAPGTEFKKAVQMAEEKGLKLVNADRDISITLKRTIRSMTFTEKAKFLGGLFL
                     SDDKDVTNESIEELKKGDMLMQLIGEMGDEFPSVKRVILDERDKYLAVNIMKNLGNTT
                     VAVVGAAHVPGIISHLQNEKTEGSDLSEIEYIPPASKVTKFLPWLIPAIIIFMFIWGF
                     INGDSSTTMEAAIYWILINGVLSALGCIIALGHPLTVLAGFIAAPITSLNPTIGAGMV
                     TGLVQLYLVKPTVKDLENASSDTLKLKGWWSNRLTRALLVSLFSSIGSSIGTFAAFPF
                     LMKILL"
     gene            complement(34966..36501)
                     /locus_tag="PL056_00345"
     CDS             complement(34966..36501)
                     /locus_tag="PL056_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPM45492.1"
                     /translation="MESFLLYSYVAVLLLLSIFGFHRYYTIYIYRKCKSNVPKPLKTF
                     EKLPKVCVQLPVYNESYVVERLINSVAKIRYPRELFEIQVLDDSNDETVEIAMLACER
                     LKACGINISHIRRPNRIGFKAGALEYGQNLTDAEFIAVFDADFLPPEDFIERTIDFFT
                     DPGIGMVQARWDHLNRDFSILTEVQSILLDGHFMIEHTYRNRSGRFFNFNGTAGIWRK
                     STIVDAGGWQHDTLTEDLDLSYRAQIKGWKFVYLPELTVPAELPVEISSFKSQQFRWA
                     KGSIQVFKKLFPSIIRSKELSSKVKMEAFFHLAANFAYVMMVFLCLLMPLNIVIRIGH
                     GIYEVMIFDLPVFVFATFSVMFFYLYSEITVINETLTMKNRSKLSPLLFLPFAISVGI
                     GLAVNNCKAVIEALAGRKTDFIRTPKYDVSGKISCRKETYKKVSLNKYRFTSVSIVSI
                     VELLFAIYFSWAVYFAFEKGFYFSMPFLILFQFGFLYTAFLSLFYTPVMRFIHREQHR
                     PVH"
     gene            complement(36549..37286)
                     /locus_tag="PL056_00350"
     CDS             complement(36549..37286)
                     /locus_tag="PL056_00350"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HPM45493.1"
                     /translation="MITDAHLHLRQLLDLHPDGDFINQSDYRAIVSCHSMEDVKVAEK
                     FSDKMFISFGVHPQDPDQSKLKILEQLILEKRIDAIGEIGFDRYEEFKENFTKQIEVF
                     EFQLDVAVKNNFPVTLHIRKAYEDMFRYSSELSKLPAVIFHSFSGTFEQAEFFLKRDV
                     NAFFSFGAAILNGHKKALKTLDKLPAERVLAETDAPYQPVSGKDFSTLEDILNVIEKA
                     SHIKGIEPSEMKNAISSNFQKILTKNP"
     gene            complement(37283..38062)
                     /locus_tag="PL056_00355"
     CDS             complement(37283..38062)
                     /locus_tag="PL056_00355"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPM45494.1"
                     /translation="MNRIHILSSGSKANAFILESDREMILIDQGLSFKEFQKRADGLG
                     VDVSKIKGILLTHEHTDHSCGIPYTAFKLGVPVYSTEKTISIINSACKYDLKYKCIDK
                     DVSFSIGNFSCIPFEIMHDAADPVGFLIRMGPDQTLCFATDTGKVTNRMMSYISRCDH
                     IILEANHDHSMLYANRKYPAELKARIRGSHGHLSNDQSLEIIDRMGGKCPRTIIFSHL
                     SEENNSPDLLSGLIEEFKKKNSMFFNSFIARQNLPFTVYLP"
     gene            complement(38063..38557)
                     /locus_tag="PL056_00360"
     CDS             complement(38063..38557)
                     /locus_tag="PL056_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45495.1"
                     /translation="MMKKGKEVGFLGSEFLLWLYWKSCEEGSLSLENLGLGEIHISLE
                     ETISLNSITGDGYSETIKSHDIAELMSVRNSIKAGRLPEIAKVRIISKELEWFFQIRA
                     TPLKISSVKLPLTGEKDENEMISMRLEALTRLDLIMQALFNTFLLEREEKSFVPDLKK
                     FLEM"
     gene            complement(38554..39153)
                     /gene="rdgC"
                     /locus_tag="PL056_00365"
     CDS             complement(38554..39153)
                     /gene="rdgC"
                     /locus_tag="PL056_00365"
                     /inference="COORDINATES: protein motif:HMM:NF016281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HPM45496.1"
                     /translation="MTKLSKGNVSVTVFKASKVPENIDETRKALERFSFKKLESTDTR
                     DESTGWVDAILCFDNQNFSSLMHDRFFVFALRNDKYSFSASQIRPYLEEAEYMFMKEN
                     GAEYIAAQQKKEIKEQVVRKLKVNSFPKTSICEVAWDMENSRVYLFSQSNQIIAKFTD
                     VFEKTFEVNLEAVSLFDSVDRMGSREKIEPVFGKIWSVQ"
     gene            complement(39231..39902)
                     /locus_tag="PL056_00370"
     CDS             complement(39231..39902)
                     /locus_tag="PL056_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45497.1"
                     /translation="MTNAGIVLNPGNIAMKNKNGGSIIVKNLNPSFYVIEAFAEGYYP
                     QKRSVVLNKGDSIYIDFKLNKIETLTEKGSVQLNFDARTEGGGSTALTSSDTLNVYIV
                     DHNSQIVKQFMDIDGSQNLNVTDLVYGQYTLKVASQKFYTLIETIVINQPSYEFFFTL
                     ATKDICGNGIIENPEECDRGMGTGGEGFVFCKDIVSDALYPDSEIQCGYDCTYDTFFC
                     YNKIE"
     gene            complement(39905..>40534)
                     /locus_tag="PL056_00375"
     CDS             complement(39905..>40534)
                     /locus_tag="PL056_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45498.1"
                     /translation="VKLVDSINTSSNTVAVVSDGRREEVPMFGSMTEFFEKVNVLVPA
                     GFTAGDTIDLVTLKIRGYGYNLIEGAGLENRPVYEIVNDMGVKTFTVKNVDPSDFAGD
                     NNGNSLSPLGTIFEGKGLDSFGDCFGEISVVVISTTNGQKQLKEKKYIVNTGSLTNAE
                     SYTGALHAPPAEGTIEVMIYDENWSPVTMTNAGIVTGLQFSSYIITGAL"
     assembly_gap    40535..40634
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<40635..43134)
                     /locus_tag="PL056_00380"
     CDS             complement(<40635..43134)
                     /locus_tag="PL056_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45499.1"
                     /translation="MFNKFKVFFIVLAVFIFVVSCDDNKKSDKDDIQISDSDTVSDEE
                     IDDAVFDDETDEQPDELTDDPVIPADEDEIADEDTVKPVTGVYGTVWIENDSTKDEKV
                     FLYECGADLAIATSDMDSSGNFEIEYVLESSKTYCVESNDFFSCFTAENDVHKAQINP
                     LTHLAFMEMHAAGGCAKLRDAEKSVRAYMKVGTGIWLGELDYSELTGIHEGFESVMGM
                     AEKSKVSEIIEAVLSDIEKNEDAEFIELFNGFKIMPEQDEVIIENVTSPVKIAPAGGS
                     TEVSPGFNLKWQVFDEITESGSGEIWSDNAGQYVVRAELLTSTGTVPISESSTTVTFF
                     KIQKEGVIDVSDMSEDMSYWFNDSSVAVFAAGTEIKKDDAAVLEIGYKLLSSGGGSQI
                     SKIAFSPSGTVFLNDMMFIMIDLDSVFSGDPIMLGVERVDGAGEMSVLNQASGDPIML
                     TASGDPIMFTASGDPIMNIASGDPIMSGTMSNVLVTATSHFSDFSVRKMSIPVKTSEI
                     LDRWENDFSIDESPYSFIYKAIELYKLNGAAEMKKFFAGRNDIREIEGDIDRVFNVEV
                     GNIRNFNIFENLYFIDSMISGFSLRKGGSGTERYTAVFNGFDIRNVIFDMYLSRMSFD
                     RSVIFPDILNSSLLPATLMKKDRTKLPDIRKSAAKGILKMNAEDVDFRYIVSKKEALT
                     LLKYVNIAKGPSFGVLNGLLLPGETVCVWLSGKTTAQCKLTPGAYSLNSAGRVTLDGK
                     EIAVSKIENVFANVLHPLDESLTDGEKHELFRTLYLILTYASNVYENGPDIVLFSEGI
                     RKVVVEMFDGIESSSKAVKLVDSINTSSNTVAVVS"
     gene            complement(43277..>44151)
                     /locus_tag="PL056_00385"
     CDS             complement(43277..>44151)
                     /locus_tag="PL056_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45500.1"
                     /translation="GIDKNSAYKYLIYSDSSGKMSVAMNEPVRFITGNPVINDKWVFI
                     NVEIKDGDPDRMMYAKIGVWKWTVIGEGDTGGDPNLVGDKLAFYDQGFKGYVCDLSKS
                     PKSRDDCIKLNREGERIATPIIDKENENLIYYENIDTSKSHKIGKVDISKTPFKYEEI
                     DIPGLSAGTKTLPVIQVKGDTILLTNVFNPVNDEAGDPQGDGKVCYYRISKKKSYCAL
                     PVEHNDGIMAYRMSYPEIEGHWLVWQDDTQPAMKLRDMECYCDHHPELCPFDDYTPQP
                     DNPKDPKTGKRLTD"
BASE COUNT        13553 a         9204 c         8687 g        12607 t
ORIGIN      
        1 ttgtttttaa aagactctct gtttttatct cagatttttt gggggaactt caaggtacca
       61 gtcgttattg actgtattaa aaaagtagat aatctttccc aatggaagga ggagagaaat
      121 gaagaagttt gcaagaattt cagttttttt gtcggcgatt gttttaattt tcatgttcct
      181 tgatttcatg cttgcaaaaa gggcaaaaga gatcatttca gatgttcaaa gcaggaaaac
      241 tgaaaagata ataattttca atgatcagcc gggcgacatc tgcaacgctt taaccggcgg
      301 aatatggctt aaacctgtca tgatttttac aaacaaactt gaaatgccgt caaactgcaa
      361 ggtttcggta acaggcggat actgctcaag aacaaaaaaa catcagatag tgttcacatg
      421 taagtgaata ttgtgattta ttcaaagagc acacttgctg taaactgctg aaatttataa
      481 agaaatcaac atttcttaca attatggaat tggattccat ttttgcaaaa cttgctgggc
      541 gtttccgatt ttcctttgct tttattgctt cttttgtaaa atcgttagaa tttttctgat
      601 ggagggatca atgcgttatt ttttttcatt gctggcaata gcagttttag caatctcatg
      661 ttcaaacaat caaaatatta aagagggaaa aagtatggtt aaagctgaag gggcaaaacc
      721 cttgtcctat ttatctgaac ttggtgaaat gatcccaaga gaagtgcttt tcgggaatcc
      781 tgacaaaaca ggggtgaaaa tatctcctga cggacttaaa atgagctggc ttgaatcaga
      841 tgaatcggga attctcaatg ttttcatcaa aaactatcct gaaggagagc tgaaacaggt
      901 tacaaaagat gaaaagcggg gcatcagaag ttacggctgg acttatgaca gtgaaaaaat
      961 cctttacatt caggacaagg acggtgatga gaactggcat ctttacattg tcgatctgaa
     1021 aacgggtgaa actaaagata tgaccccttt tcccggagtg agggcacaaa acctcattct
     1081 tgacagaaaa tttcctgaca aagctctgat cggacttaac aaaagggatg cggctgtttt
     1141 tgacatgtac cgtcttgacc tgaaaaccgg agaactcgaa atgacagcag aaaatccggg
     1201 tgatgttgcg ggctggggta cagatcacga atttaaactc agaattgccg ttgccaccga
     1261 tccggttacc gcagacgaaa ttgtaagaat acgaaataac gaaaccgaac cgttccgcga
     1321 attgataaga ataccttttg gcgaaaatgg cggggtcatc aattttgcac ccgacaacaa
     1381 aaacctgtac tttctcacaa gcacaggcag tgatactgtc cggcttgtcc ttcttgacgg
     1441 agagaacgga gcagagctta aaaccatatt cgtcaatgaa aaagcggaca tatcagatgt
     1501 aatgacagac cctgacacgc atgagatcct tgcagtttca gtcgaataca caaaacttga
     1561 atattttgtc atggacgaaa gtgtgaaagc cgattttgaa tatctttcag agctaaaaga
     1621 cggtcagttc tacatcgttt cgcaggacct gtcaaaaact aaattaattg tggcatatat
     1681 gcttgataac gctccgtaca gttattattt ttacgacaga tcttctaaaa aagccgattt
     1741 tcttttcacc cataatcagg atcttgaaaa gtataaactt gctaagatgg acccggtgat
     1801 aattaaatcc cgcgacggac ttgatcttgt aagttatctc actctgccgc tcaatgtaaa
     1861 gccggaaaat cttccgctcg ttcttgatgt tcacggcgga ccatgggcaa gagacagttg
     1921 gggttacaat tcagttcacc agtggcttgc aaacagaggg tatgccgttt tgagtgtgaa
     1981 tttcagatca agtaccggtt ttggcaaaaa gttcttaaac gcaggcgaca atcagtgggg
     2041 aaagaatatg cagaatgacc ttactgacgc agttaaatgg gcaattgaaa aaggtatcgc
     2101 cgataaatca aaagttgtaa taatgggcgg cagttacggt ggttatgcaa cacttgcggg
     2161 acttacattc acacccgaac tttatgccgc cggagttgat atagtcggcc cgagcaacct
     2221 taaaacactc cttgaaacga tccctccgta ctgggcaaca tttaaaaagc agatggtact
     2281 ccggatcggt gatgtggaaa ataacgaaga gcttaacaaa gaaatttctc cccttttcca
     2341 cgcttcaaaa ataaaagcac ctttgatgat aggacaggga gcaaacgacc cgagagtaaa
     2401 aattgccgaa tcagatcaga tagttgcaga aatgagaaag aacgatcttc cagtcacata
     2461 catcgtttat cctgacgaag gtcacggatt tgcaagaccc gaaaacagga tggatttttt
     2521 cggcagagta gaaggatttc tctccttcca tatcggagga agagcagaaa aatttgaaaa
     2581 agatgacaaa agttctgcac ttgagaaata actgaggtct catggcactt tcgtatcctt
     2641 acatttttct tcttgcttta agtcttgcga tggatgcttt cgcagtgagt ataagtgccg
     2701 gaatttccat gcagaaaagg gatttttcag aagcgttgaa gattgctttg gcatttggaa
     2761 tttttcaggc agttatgcct attatcgggt ttttcggtgc ttcatcattt tatgactata
     2821 tctgccatgt cgatcactgg atagcttttt ttcttcttgc cttcatcggc ggaaaaatga
     2881 tttctgaagc agttaaaaag aaaggtgaat gcgaggtttt tccggcaaat ataaagtttc
     2941 ttcaattgat ggtgctcgca gttgcaacaa gcatcgacgc tcttgccgca ggagttagct
     3001 taagcatgct ctgcagtgac attcttattc ctgtcctgat gatcggattc gtgacatttc
     3061 tcttttcatt ttcaggggtt acattcggag caaaactcgg atgcagattc ggcacgagga
     3121 tggagatcgt cggaggaacg gtgctgatcc tgataggtct taaagtactt cttggagata
     3181 ttgcgggaat ttaaggttca tattcaatgt ttgcaaaaca ccgtttaacc cttttactgc
     3241 agaaaaaccc tggctcacaa tctgaatttt ctgaacaatc gccctcatat ttatcaagaa
     3301 tatccttagc gtctataact cttaatgtgt ttgttcctga tgttgtcaga attgaattgc
     3361 cggaagaaat acctgttgaa cttatgccgt aataatgtgt ttcaaaagga acagtttcac
     3421 cacttgaaat ctcgtaaatt gcagaatcag taacaatgta tttttcatct gatgataaga
     3481 tggctgtttt tgtatttata ttaaattgtg gctccgtaat ttctgaaccg tcatctgaag
     3541 agtaaagttt aatatcaccg ttggttacaa ttatttttga tccgtccgga aaatacgaaa
     3601 cattcccgaa aggaagtgaa aaaatttcat tccattccgc agtatcgtaa atcttgttcc
     3661 cgttcttatg cgttacagcc gcaatctttc catcgggcga gaatttcact gctgaaactt
     3721 tatcgctatc atcaaaagaa attttgtgca gctcttcacc tgttaaaatc gaataaaatg
     3781 aaacatcgtt aatgtcatat cctgaccatg cggcaagtat cttatcatcg ggtgaaaatt
     3841 caaagtaata atattgggga cttttttcaa atacaactat gttatctgtg aaattgtaca
     3901 gagcaaatcc ttcatcattg taataatcca gcaaaaaaac agccattttc aaatcatctg
     3961 aaaaagcaaa gatgtagtag aaaggatcga acgggagcgt tgcttctttt atcacttcac
     4021 cggtcagcgg cttaataaaa ataacttgat tgcctcttaa aaaaaccgct tctgactcat
     4081 ccgatgaaat tttcagatta ttgcttccgt tatacctgct tgaaaaatat ttgttaaaag
     4141 aaggaagcga atatttctgt aattcaccag tttcacttcc tatcacaaga gcactgtcat
     4201 ccggagcaaa ttccatctgc caaccatctg taagaattgg ataaaacctg tcataaagag
     4261 gatatatgaa cgaatattgt ctgccaccca cagcaagaaa atcactgctt gcattaaaag
     4321 aaagagaacc gatttcatca gaaggcccct gcaatgaatg actgaatttc cattcactgt
     4381 cgatgtattt aaaaacatta acattatcat catattctct tggatatcca ccggttgcta
     4441 taatattgct tgaagaaaaa gctacctcca cataatactc atcaaccgga agaattttta
     4501 aaacctcttt ttccttcagg tcatagatca tggtgttctc ataaaaagat gaaagccctg
     4561 aacctgcgac gagatatctg ctgtctttag aaaaatcaat cgattcaaca ttccagccgt
     4621 aaccggtttg aaaaggggtg tccgaaaaat agtgactgag tgaatctgaa gttacatcat
     4681 atactgtaaa gccatcacta tctccaacag caagcattgt tccgtcagga gaaaacttta
     4741 tcatttgact gtaactgtga taagcactat ccagaatagt tccgtcctcc atactcctca
     4801 aaacaagtgt gtcccactca tctgcagcaa gaaattttcc atcaggcgaa aatgcaattg
     4861 ctgttgaagg gctgaaggaa accctgaata tttccttaaa attatccgtt ctatgaagac
     4921 ggatcgcccc catcctggct gtggcgatta gagaactgtc aggagaaact gcaaaacgac
     4981 ggatctcttc actgccgtac atctgagttt ttctacagaa agaaccttcc tgaagcaatg
     5041 attccataca aagtccgtca aaacagcatt cttcgctatc tttacatggt tctccaattg
     5101 caacgcaacc ccatttttct ccaaaaagct cacactcttc tccgctccat cccggataac
     5161 atttgcattt tccttcaaca atttcagtcg cacatgaaca ttttccatca ccgtcaagcc
     5221 cgtcattgca gacctcattt tttttgcatt cacacggctt gcaattcttg ccgaaatgcc
     5281 ctttctgaca ccggttgcac aacttgccta tccagccatc attgcagaaa catttagcgg
     5341 ccagatcgaa attataaaaa acatcataca gttcgatgca cacatttttg ttcttttctg
     5401 tacatggatt aggattgcac aaagttgcgt caatatcgga atcgctgttt tcgttatcag
     5461 tttcagaatc agtgtctggt gtctcttcgt tttccgccat ttcctcattc tcagaatttc
     5521 cttcctgttc aaatgttcca tccgagtctg tctgaaataa atcatctgaa tctttaaaat
     5581 catcgcttag ttcggaattt tgatcatttt tgctttccct gccattgtca cagccggtaa
     5641 aaatgagaaa aaagactgtt gttaagatca tgatgccgtt aaaaaaaact gacataattt
     5701 atcctccttc aaaccagatt ttgcaattta ttaatattga ccggtttgtg caaaacagcg
     5761 tcctgaagct gtgttgcaga aaaacccggt gtcgcagtct aaatcatttt gacattctac
     5821 ttcaaatttt tccagggagt tgcttgtgtc gacaaccctt gcaaagtagg ttccgcttaa
     5881 gaaaagaagt gtgctgtcag gtgaaagagc gatcttatca atgtcaaaat caattttgtc
     5941 gaaagaatgc agtgtcgaag tgcttatatc aaaaatacct ctatcaaata taatatactt
     6001 ctgatcattc gatataacgg cttgttcaga aattattccg ctgaaagaag ttgatactgc
     6061 atttccgtca actgtagaga aaacattcgg catcagatca attatcataa atctggatcc
     6121 atcaggaaaa aaagaagtgt gtcctgaaaa tggaacagaa tagatctcca gtcctgtttt
     6181 catatcatac agagtagttc tttcttcaaa accgactgca agaagcaatc catcgggcga
     6241 aaaaacaaga tctgaaacca ggtcttcttc gttatgttgg attgaaaaaa taagatcacc
     6301 tgaattgatc aacctgagat gtgttgtaaa tgttatcccg tcatgaacgg caaaaatttt
     6361 atcatcggtt gagaaagcag ctgctcttgc cattatttcg tcttcattgt agattatttt
     6421 gcctgtttca aggtctgtta taaaaaaccc tttccttctg tagtaatcat ctgcatagga
     6481 aatgctgtat gcaccgctgt cagacaattt gaaatttaaa taaaactggt tcagatcttc
     6541 cattttaaac cgtttgagct cattgccggt aagaggatca gaaaataagg tttcatagga
     6601 tgatgtaaaa cttactacac tttttccgtc atgagagaaa aaatgtgcac gaccgcctcc
     6661 ttttgtgccg gattcaagaa attcaaatcc gggaattgaa tatttgtgaa gagctccctc
     6721 gccaatggaa ataaggttct gtccgttttt tgaaaaaatt atgctggaat ggtttgaaac
     6781 aaatggaaaa aagtgacctc caaccctgta aacaacattg tcaacaagaa gataatctcc
     6841 tgaataatta aaagacaaag aggaaagaga atcaagcgta tagtttatat gttccttgag
     6901 actccagtct gaacagttgt aaatgtatgc aggatcttca aaccagccat ctatcgcacc
     6961 gtcaagggaa tatcctccag ctgcgatata tcttccatct tctgaaaacg acaattttgc
     7021 atagtattca taaagtggta gttcatggat cagttttttt tctttcaagt cataaatgtg
     7081 aatgttgtcg taatccatag atgctccggc acctgccgca acaagagaac tgtctgcaga
     7141 aaattgaact gaatatgtat ttatcccgat agcaatgttt tcaaaagaat cagaaaaata
     7201 aggttcaaat tttccgttat tgagcatata tacatcaaat ccaccccagt ttgttgctgc
     7261 aagcattgtt ccatcgggtg agaatttaag ttcttgacag taattcagcc cgcatcccac
     7321 agcttctttt tctccgttat caagattata aagatacagg catgtgtcat ttacagcagc
     7381 aagaagtgac ccgtccggtg aaaaatctat gtttgccgtc ctgccttcac ctaatatatt
     7441 gatttcttta agtgtatcaa tgtcataaaa atatatgaat ccggacctta cagcggcaat
     7501 taaagtgccc tgctgagagt cggcaagcaa agccgattca ctgtcataac ggcgggcttt
     7561 aacacatttg gaaagaatac agactgaatc aaagcaacag tcaacgcctc catcacaatt
     7621 ttctccttcc ggggcacaaa tccagttttc accgaaaatt tcacaatcat caccactcca
     7681 cccgggataa cacgcacaat tttcatcaat aatttcagtt gcacaggagc attttccatc
     7741 accttcgagc ccgtcattgc agacttcatt ctttttgcag tcacacggct tacagttatt
     7801 tccgaaataa cctctctggc agtcatcaca tttcggtccg aaccaccctt tatcacagga
     7861 gcagtaatat cttcttttgt cagtgcgcca tatatcgatc tctatgcaga cagatttatt
     7921 cttaaaatta caagggtttg gatcacacag attttttaca gcatcggcat cactgtcttc
     7981 gtcgtctgtt tctgaatata tatcagccgt ttcatccggc tgttccgact cgttgtactc
     8041 attgtccgct tcgtctgcat cttgctgatt ctcttcatat ttctcatttt cgacaaaatc
     8101 gttgtcatat atatccgtgt cattttcatc attttccccg acttttgatg attcgcaacc
     8161 ggcaaaaaga acaaacggaa taataaataa aaatatccgg cgactcgtaa acataataac
     8221 tcctccgata aaatgtgcct gccgcacttt tatcataaac tttaaataaa ttcaaatata
     8281 ttagatatta ttacaataat gtaaccttaa taattcttgc ttaataatac cttttgtgtt
     8341 acattcggtc gctttatttt tggtaacctt gaaaaatgga ggcgactttg aacgctaatg
     8401 tattgaaatt actgggggat ttggcaaccg gccaggcggc tggagcaggt ggcggagcgg
     8461 caggtatttt aaatatgctc ctcccgttta tactcatttt cggaattttc tattttctgg
     8521 taatccttcc ccagaaaaaa cagcagaaaa aacatgttga aatgcttaat cagcttcaga
     8581 aaggcgacat ggttctcatg aactgcggaa taaaaggaag gatctcggag ataagaaaca
     8641 acgacatcaa acttgaaata tctccgaaag ttatcatcac cattcagaaa ggcgtaattt
     8701 cgggcaggat cactgcaacc gaagaggaag aaaaagaaaa ctgaaaaata atggagattg
     8761 gttgttatgg aaagaaactg gttggcaaga gcaattttca ctctggcact tatcattgtg
     8821 gctgttgtta tgcttattcc cacatttgta gaccaggaaa agaatccctg ggtttcaaag
     8881 ataaatgacg ggataagtct gggtcttgac ctcaaaggag gcattcattt tgtgctcagt
     8941 gttgatactg aaaaagcggt acaggacaga cttgacagaa gaactgacga gataagaatg
     9001 cgtcttgaag aggagtcact tcccttcgaa tctgttactg ttgagccgga aaattattca
     9061 attattataa agatcactga aaataccgac aaggacaaat tcagggataa aataatcgat
     9121 ttttttaacg acttcagaaa aacttcaaca gacggtaata ttcacacaat gaaaatgcgc
     9181 gacgatatga tcgcacagat aaaggaaagt tccgttgaac aggcactcga aactctccgt
     9241 tcaagaatcg atgaactggg attaaaagag ccgatcgttg caagacaggg tgaaaacgca
     9301 atacttattc agcttcccgg atacaaagat gttgaacggg caagaagcat tatcggtcag
     9361 actgcccagc ttgagttcaa gatcgttgcg gaagacatgg atccgcttgg cgaatatacc
     9421 ggtgaagtgc ccgaaggcat aacacttgtt cagggaagag gaacaaactc cgatggatca
     9481 gttctgacct ataaatacgc aacaggtaaa gaccgtgtag caatgagagc ttttcttaaa
     9541 gacaaagctc cggaaggaac agaattcctt cttgaagaga gcgtttttcc aaatggcgaa
     9601 aaggaatatc agtcatacct tctctacagg caggcctata ttactggaga catgctcaca
     9661 gacgcaaggg tttccataga tgaacagaag aacagacctt atgtaagcat gacttttgac
     9721 aagaacggtg ccagaatatt tgctgaagtt acaggcaaat atgttaaaag aaaaatggct
     9781 atcgtccttg atgaaaaagt gaacagcgct cctgtcatcc agtcaaaaat agaaggcggc
     9841 agtgcaatga ttaccctcaa ttccatggga gattacaata ccgtttttga ggaggcgaaa
     9901 gaccttgcac ttgttctgag agccggctcg ctccctgcac ctgtcacatt tgaagagaac
     9961 aggacggtag gaccgagctt gggacacgac tcgatcgaag caggaaagct ttctgttatt
    10021 ataggattcc ttgccgttgt aatatttatg ataatttatt atggactggc aggcatcgtt
    10081 gcaaacacag ctctttttct gaacatcctt ttcataatgg cgggcatggc tgctttcggt
    10141 gcgacactaa ccttccccgg aatcgcaggt atagtcctta cgatcggtat ggcagttgac
    10201 gccaatgtca tcatttttga aaggatcaaa gaggaactgc gtctgggaag atctctcgct
    10261 gatgctattt caacaggtta tgacaaggca tggtcagcaa tacttgacgc caatgtcaca
    10321 acagcgatat cggctttcat tctctgggaa ttcggaacag gtccgatcaa gggtttcgct
    10381 ataactcttc ttatcggtat cctttccagc atgttcactg ccatatatgt gaccagagtt
    10441 attttcaact atatgatgaa ggcagggctc aaaagattct cgtacagaga taagaactga
    10501 ttgatggaga caaaaatgac aatatcaaat agaaacatag atttcatagg aaaattcaag
    10561 cttttcggaa caatatccgc tgttgcagta accttaagct tgatcgtgtt ttttgcaatg
    10621 ggtctcaact acggagtcga ttttgccggc ggaaccgaaa ttcagatcaa ggttgacggc
    10681 atgaacaatc aagaactcag aactatcgca agtgagttcg gaagtgttga tatacaggag
    10741 ttcgaaggga gagataatga gtttcttctc agatttctca atatctcaac ggttgatgat
    10801 gctacaataa acagttttgt tgaagaagtt aaaaatgaat tctcctcttc aaaaatgaca
    10861 aaaaaacatt ttgactctca ggttggtgat agagttgagg tttggtttga gaacgagatc
    10921 gacatggaaa aactttcctc aatgggactc aatcacaaaa ttcctgcaac aggcaagatt
    10981 gaatacaaaa aagtcggtga aagacacata tacagaataa tgctcgaagg tctcaccacc
    11041 aagatcctgt catcaataaa ggagaaatcg ggcaaggaac ctgaactcat gagagtcgag
    11101 ctggtcggac ccaaagtcgg ggaaaaactc agattttcag ctttccaggc cgtgctttat
    11161 tctcttattg ccattcttat ctacatcgca gtaagattca atttccagtt tgctcccggt
    11221 gcagtaattt cacttgcaca cgatgtaatg ataacactcg gtattttttc tgtattcaga
    11281 ataccgtttg atctgacaat tgttgccgca ctgcttacaa tagtcggata ctctcttaac
    11341 gatacgatcg tcatctatga cagaataaga gagaactgga acaatcctaa aaaaggggca
    11401 agtgttgttg agaagatcaa caccagtata aatgaaactc tttccagaac actgctcact
    11461 tcaataacaa cacttatcgt ccttgttgct cttcttattt tcggtggcgg aacaataagc
    11521 ggattcgcac ttgccatgac aatgggtgta gttgtcggaa catattcatc tatcttcatt
    11581 gcatctcctg taaccgttct cattgaaaaa tatatgggaa aggagaaaac agcatagatg
    11641 aaagaaaaat ggcagatact gaggcctgat agacagaaag ttgataacct ttcgaaaaca
    11701 ctgggtattt caaaaaccgt agctactgtt cttgtaaaca ggaaaattga cggtgttgaa
    11761 tcagcatccc ttttccttca atcaaagata aaaagcctgc cggatccttt tcttttgaaa
    11821 gattcggaac aagctgcagt aataataaca gaatacataa agcagaaaaa aaagatcgtc
    11881 atttacggcg actacgatgt tgacgggatc acttcgactc tgataatgta ccagttcctt
    11941 acccatgtcg gcgccagggt cgatcactat attccgcatc gtcttgagga tggatacgga
    12001 ctgaacacat atacacttga agagatagca actaacggtg gcgaacttgt catcacagtt
    12061 gactgcggca taacaagtgt cgatgaagtg aaatatgcaa aagatctcgg gcttgaaacc
    12121 ataattattg accatcacca tgtcggcgaa gaaataccgc cggctgttgc tatagtcaat
    12181 ccgcatcagc atgactgccc ttatccattc aaagaaatgg ctgctgttgg tgtcgcattt
    12241 tcttttctga tggttttaaa gaaagttgtt gaaaaagatg gttttttcaa aggagcactg
    12301 cccaatcttg ctgaatatct cgatatcgtc gcaatgggaa ctgtggctga catggtgccg
    12361 cttctagatt cgaacaggat ctttgtcaag cacggtctga cccagatgca gaaaaacaaa
    12421 agaccgggac ttcttgccct tgcaaatgta tgcggactga aaaatctaaa agagatcact
    12481 cccgccgtaa taagttataa aatggctccc cggctcaatg cggcgggtag gatcggaaat
    12541 gccataaagg gtcttgaact cattgaatgc accgattatg aatccgctta taaaatggcg
    12601 gaagagctta atctgacaaa tgaataccgt cagcagctgg aagctgaaat attcgaacag
    12661 gcaatgtcaa aggttgagaa cagtgatatt atcgatacaa aaagtgctat aattctctac
    12721 tctgaaaaat ggcatcccgg agtaatcggc atcgttgctt accgtctagt tgagaaatac
    12781 tataagccaa cgatcatgat ctcggttgaa gacggaatcg gtcgcggaag tgcaagaagc
    12841 atacctcagc tgcatattta taacatactc aaagaactca gcagctacct cgtacagtac
    12901 ggcggtcaca aatatgctgc cggtctcact ataaaagaat caaacattga agagttcatt
    12961 gaaagattca acggtatggt cgatactgct cttgctcagg aaacctatag ccagtcaacc
    13021 gatattgatg ctgaacttcc gctgaaagaa cttacacccg atcttattgt ggccctttca
    13081 aaacttgaac cgttcggaat gaacaatcag gaacctaatt ttctggcaac aaatgtgcag
    13141 atcataaagc agagtgtcat caaccggagt catcttcact ggaaacttgg tgtggctgat
    13201 tcaacaatga cattcaactc gatcggtttc ggacttctta atgaaaagga ctcaccgaac
    13261 actggcgacc ttatcgacat tatatatttc cccaggataa atacttatgg cgggaatatt
    13321 actcttcagt tatatatcaa ggatttcgta ttttctgaaa aaaaggattg atcaatcttc
    13381 ccttgttctg aacacgagga acattccgaa taccatggca atgattactg ctaaaggacc
    13441 ttctgtttct tcaacgacgg ttaaggaaca ccctcctttc ttgcccggct catcagtcac
    13501 acatgtatta ggatcggcat catcgcataa atatttgaat tccttggatg aaacaggaag
    13561 ttcagcaatt tctctatctt cagtcagact gaccttgctg atcgtaatct tggctttgtc
    13621 accttctttc atcttgaatc cgcacttgaa aagaaggtat ttctgctcat ctttactgta
    13681 agaaagaatg ctgtccagag aactgatcgt aaaagatgtt ggattttcga actcagttat
    13741 cttctggata agagtgtctt cgggatcgtg ttcaccgtta ccgttatcat caagtatcag
    13801 tgtcactgat tcgatcccct caccgaatct tgcaaaacct gtgcttgatt ttatgaagat
    13861 cgattttatc gcatcaccgc cttccatgga ttttgttctg acctgaagaa ccgggttatc
    13921 cttgttcatt tctttaaaag aaggaacctg cgggtcgttt tcgccttttg taaatatgaa
    13981 accttccggt ggctcgaacc tgaatgtcgc gaattcaaaa ccctctccgg aaacgacagg
    14041 ttcacctgaa tcgctgatcc ttattgcctc cttgttctgt atttcagcat aaaaactgcc
    14101 tgctatacct gatgttatat tggttgaaac atctgcaaca attatgaagt tgttctttat
    14161 tcccgaagct accgccctct tgcctgtatc aatgatatca aactgtgcat aattatctcc
    14221 ggtcagcgca tttgtttttg caagctctgt cttcgaatct gaatatgctc cgttcccgtt
    14281 gtcatcggca atgagtctga tgttggatat tttaacatct gaatcgtatg taaatctcac
    14341 cgctacacct tcaagatcat aagttttccc ggtgtcacct tcagatatga gataaaactg
    14401 tccgacaaca agatccttct tgggtgaagg gattattatc gttgttgatg aatcaggaga
    14461 gtttaaacct tctccaaatg acacctgagc atcttttgtt ttatctttgg gctcctcaac
    14521 gcattgatta tcaatgcact ttttaccgtc cttgcagtcc ttgtcttctt cacagtaatc
    14581 atccgtacct tgtcccacgc cgccgcactg agctttcggg ggaagactgc attctgtaac
    14641 agcgggacaa gtgccgttct taagacgcag cgcgatgttg ctgtttgtgc tgtaaggaag
    14701 tttacctgca tctgtgatta ttgcactgtt ccttattgtt tcatgtttag gaagaccggc
    14761 tttcggtctt acaacaaatc ttatcatgac tgtgtcggga caatccattg tggttttatc
    14821 gcagtaaccc atgagatcag aaacctgctt tgctgtcgcg aacggaaaat ttccgtcaac
    14881 atcatcaacg gctttccagt cagttcccag gccatcctta aaaactgttg ctatttccgt
    14941 tgtccccttt tcatattcga catttgaagg aagtgtgtcc tgaaccgtta cattttcagc
    15001 aagattttca ccccagttct gcacttttat gagatagtaa aaaggtctgt caaaacatac
    15061 tgcatccggc tcagttgaac atgaacagta gtttttctcc cttccgtccg gtgtgtcagg
    15121 atttgccggt atgtcaaatt tgggtgcctt cgtatcaaca ctgacaacaa gaagattggt
    15181 ataaacctga tcctgatttg cgcctatttt aagggagaac atcgtgtccc ctttcttcag
    15241 atgctgatcg aaaggaaatt ccttcgcctt caaaaggaaa gtgtcggcat ctatagcata
    15301 ttcaagttta gtcggatcag gactgttcgg gtcgcctccc acacagacag gcatctcatc
    15361 ttcccatccg aaaatggatg aaatggagtt gtaaacctca gtataggcaa gcggtgtata
    15421 ttttgcagga ttacagttat tccagagaag atcaaaattg ggatttgact gaccgcttat
    15481 cgcaagtgcc tcaggaggag ctggctgaaa tgtatctgtc gttgcactgg caagacccgg
    15541 gtctccttct gcaagaacca tcgtcattct tacttcagct tcgttgggaa gctcaaaacc
    15601 gctcacatca atatatgcct cctgaaaacg gtatgcttca agtccgttat agaaatatat
    15661 tttcttgggg cttatccttt ctgaagtata aacaaaaaca agagcccatg ctccaaccat
    15721 gccgcttgtt gtaagatatg cctgcgctct cgtgcaattc atccccttga cattatagct
    15781 gccgtaaagg gcttctccgg tctgagtaat atcatttaat gcaccaagtt cgattatctg
    15841 ctgcatgaat tcagtcacat caactctgta tgtgaaaatc cctgtgctgt cggatggttc
    15901 gcttatcgct tcataatcaa acgaaggagg ggtaaaaaga tttcctgaaa cacttgatgt
    15961 cacctcagtt gaaagtgtca cttcagaagg tttgctttcg tttgtgaatg taagcgttac
    16021 agagttgtct gtcggaccgt tatactgaga tggatcctgt gctgcgatcc agatgagaaa
    16081 cgccctgtca atgttcgtgt caggcggaac atgcgcagaa gttaaagtaa atgtggagcc
    16141 gacattaata tcaatgcagg aatctgcctg cgggttgccg ggagttgttg tcacttctcc
    16201 tgtcacctgt gctatcagtg acttgaacat cacaaaataa tcccacgcac cctcttccca
    16261 tttcagttcc tgaccgttag tctgggacac attcggtcct atgacagcgc tcaggaaaaa
    16321 agggaacaga gcgatcataa ccataaaaat ccttttcatg aagcttcctc cggtaaaatt
    16381 tacaaaacca acatctttat gattatagat acgatcattt aatatgtcaa attgaactcc
    16441 cggcataata gttggaatag ttggggtcag ttattccctt taaagtcaag aagtattata
    16501 ttcagaaatg acttacatgg gaataaattc ttaacaggac aggttattaa tatcagtaaa
    16561 taaacggaaa cacccttttt ctggttgatg ggtatttttc gccgaaggtt tctctatacc
    16621 attttctgtg tttgtgtgct ctggggacga gatttgccat tgtgaatacc atgaatgcaa
    16681 gtccgggaag agaccatgtg aggatggcat aacctgtcca ttccattatt tcgccaagat
    16741 aattggggct tgcgacaaat ttgtgcatcc ctttttcagg caggtggtat ccgggacctt
    16801 tttcggctcg aagtgctctg actacatggt ctgagtggat gtttatgacc atacctgaga
    16861 aaaagataac cgttccgatg atgaatctgg ggtcaaaaaa ccatgaaaga tcgtagtcgg
    16921 caaggtgaaa aagatagtat ccattcagaa atccgttcat gctgttgaac attatggaaa
    16981 agatcatgat gagaaccggc atgttctctt cacctttgat gagaaaggga taaataaatg
    17041 ttctctgaag atagtgaacc atccatattg tgagcatgac taccggaact gcttccggct
    17101 ttcctgtaaa cagaaaataa agcgggatga aaacaacagg ggactccatc accatccagc
    17161 cgattctcgg gtttattgac ggtccccatt tctttccgcc gtgacggccg taacccgctg
    17221 tcacaaaata aagtgcaata aacacaaatg cggcactgat aaactgtgcg gcaaggagca
    17281 gatcataaac agttttcatt tccccctcct attttttctt tcgtggaagc ggcatcggct
    17341 caatattttt gttttcaaag ttaaatatca gaattctttt gtacatcccg agaacattga
    17401 gattcacttt ggtgtaaccg cttgtaaaat ggaaataatc tccaaaatct ttcgcctgat
    17461 atttgacata gagatcgctc actccaaatc ttgattttcc cgaagtacct tcattttgta
    17521 taagttcgag attgtcaact gtaacccata gaaacccctt gaaatgttcg tctttgggct
    17581 tcctgggagt cgcctttatt ttataacaaa gcttcccttt cactttttca actgccgcaa
    17641 gttcccggat atattccttt tctccgttct tatcaaagtg gggaatcccc ggttttgatt
    17701 ctctcggatc atattttgac gggtctttct tttcaccgtt tacagtatat tcaagcactt
    17761 catatttaag gtcttcataa aagtcatccc atctgacata aacaactttt tcagtttcga
    17821 tcaactcgtt ggtctttgga tgccgccact caactgtcac atgtcttgtg cttttgaccc
    17881 cggcatattt ttcatacatg ttctttgaat atttttcagt aattgcgtca attacctttt
    17941 ttgaaagttc cggcgttatc accatccctt cagtaacatt ttcagttgcg tgaattaacg
    18001 gaaaaacaaa gaaaataatt acaaaaagaa aaccggaaaa caggtatttc attacaatcc
    18061 ctccacaaaa aacagcaaca aatctttaga ccgtcgctct atatcacagg tttcataatt
    18121 gaacaaatat ttgttgagaa aatataataa aagatataca atggttgacc taatccggac
    18181 tggaaggtag aaattttgaa ttggggagat tctgtttgaa actggaaaag ggaaccacgc
    18241 ttcttgaacg atttgaactg acaagaaaac tgggaaatac cgctttcgga gatgtgtggc
    18301 tcgcagagga tgcaaaaaaa gggtcacagg taactttaaa gatcatcagc tcaaaatatt
    18361 tcaacaaaaa gacagatctt aaaaagctga agaacgaatt tcgctcattt tcgcagataa
    18421 aatgcaagca catttcggaa gtatacgaga tctttgatga aaaaggtctt ctgttttatt
    18481 gtgccgaata tgtcgaagga tcaaaaatat ccgaattcag gggcgaacat ccttccgtcc
    18541 ttatcccttt tttcatggac atatcaaagg cattgctcga acttcacaaa tacggaatag
    18601 tccatggagg gataaaaccg ggcgatatca taataaaatc agaccgttca gtaaaactgg
    18661 tcgacatagg ggtgctttct tcttttgaga acaggaaccc tcagatcaga aaagttttca
    18721 actacaccgc tcctgaaata atcaacggca aggccttcga ctggcgggcc gacctttatt
    18781 cgctgggcgt cataatgtat gagatacttt acacaaaact tccatgggtg gcgccgccgt
    18841 tcctggacaa cagtgaagaa atctcgattg aaatatcgtt cccgaagaac agctctcctc
    18901 agataaacgg ggttataaaa aaactccttt ccatcgagcc ggacagccgg tttaaagatg
    18961 catctgagct tatcagttct cttgagcata tatttgcggg aactgcaaaa aaagcaggac
    19021 attcagccaa tgtaaaaggt gttttgaaaa taaaggagac cgaattcatc tcccgccgta
    19081 ttgaaatgac cactttgatg gaaatgctgg ataattttga agccacatca atagacagct
    19141 cggtaataat agaaagtccg gggggagcag ggagaacaag atttttaagg gaatttgaaa
    19201 aaaggatctc ttccagaaat cttaatgccc tttttttcag tgcaagtcag tcacagaacc
    19261 tagcaactga tattgttgac accatctggg aatcacttga cagggaatac cgtcttcagc
    19321 tctcactcaa gtggagaggg acggttcttc tctactttcc tcatttcaag tcatacggtg
    19381 aatttaagaa ctgccctgat tcaaagcatc ttccgctcgc aaacgaagat ttctaccgcc
    19441 ttgcgtcatt agtccgcgat ttcattaaaa tgggaacaag gaacagaccg atagtaatgc
    19501 ttcttgacaa ctttgaccat gttgacaaaa gatctctccg tctgatacag gaaatatcgg
    19561 cagggtttga caactccatc cggctcttta cgatcgtcac tctcgaccct gcaagcggtt
    19621 ccaacattga gatccctgca ttttcaagaa taactctcag cccgttcact ttttcagaaa
    19681 caagggattt tattgaagat tctcttgatc agccggcaaa tcagatagac aatgaacttt
    19741 ttctgtggct gtatcgtaac agcaagggac ttgcaaaaca gatccggtca cttctctttc
    19801 tccttattga ggaaaaagtc atctattcca gcggaaatca tattttcttc aactatacag
    19861 atatgggcga aaagggaatt gatctcctgc ttcagaataa aataaactct ctttcacaaa
    19921 atgaacagtt gactcttaag gcatgttcaa tattcaagaa gttcggaact aaagaggcac
    19981 tggaatatct cataaaagat cttatgaccg tcgaggagct tgaatcagcg atccgcattc
    20041 ttgaagccaa ttatcttatc gttgtgaaca aaaatgggaa aataaccatc gtgaacagac
    20101 agttcagacc gataatttat gaaactcttg aagaaactga aaaaaagaag ctccataaaa
    20161 aaatgggtga ctatatctcg ctgacatggc aggatcttct ttcgatgaac ataaaccatt
    20221 tcgcatttgc ggcacaccac tataatcagg cgggagcatt tgaaaaagcg ctgaaactct
    20281 atcttttctc tgttggcgga tcaatggctc agttcaactc cgaatttgct gaatcctcaa
    20341 tagatgacgc acttgaaata gtgactttaa atcccggcat agtgaaaggg aaaaagcttt
    20401 ttgcaatata tcttttcgcc ggcagaatgt actataagct tggaatatat aaaaaagcgg
    20461 ttcctcttct tgagaaaagc tatgaagtct ggaaagagga tactattctg gaagaccttg
    20521 tcttttcact ggcaaatgat tcagatcctt caaaagctgt aaagtttata agcagttacg
    20581 gcgacgagac tcctgataaa aaagcggtca aacactatct cagatcgttt gtgtatctta
    20641 caactgaaaa caactataca aaatctcatt atcaccttaa aagagctgtg ggctatatcg
    20701 aaaaaggtta cggaaaactt ttcggaccga taaggaaatt cacactgaaa cagcttcagt
    20761 tcgaccttga tcttttcatg aacacatcgg gatttgaaac tcttgacaag ttgaggatcg
    20821 aacttatcga aagctcaaag aacattgaat cgaaggcctt tctcatagac gcaatgaatg
    20881 cctctttcct tttctactgg aattacaacg aaatgaaaaa ggcatacaac atactgcatg
    20941 aatctctgaa actctcgctt gaaatatttg ataactacag aataaccaga agctatctga
    21001 accttgcgaa ctgttcccac cggctgggtc gtctcaatga tgtcagattc tatcttgaca
    21061 aggccattga gtatgcaagg aaagggagcg gtgcaagcat cctgaaacag tgttactgca
    21121 actacggtga gctcgcaata ataaaaggcg agttctctct tgctgagaac tatctgtaca
    21181 atgcagagga aatatcagaa agagaatacc gtgatcctga tctcattata atttacagtc
    21241 tccagacaat actttcgctt ttaaagaacg agatcggcac agcaaggaat accggcggaa
    21301 agctgagacg ctacatcgag cagttcagaa ctgtcggaca gaacaagcag atatcttatt
    21361 tttcagcgtt aatgctcctt gaagccatga caggtaacga tcatgaattc ttttttgaac
    21421 ttgacaataa attccagaaa ctgctgaaca gctatccttt attcaaaaaa tccaacgaac
    21481 ttcttcacat aacatgcagg atcatttttt tcaaacggaa caacgaccgg gacagcgcga
    21541 ttaaccagat tcacgaagtt gagatcaaag gaatcaaaag tacccactct ctttaccaga
    21601 tgatctatta ttaccatgca tcacttctac ttaaggagtt ctcacctttc agcggacttt
    21661 tgaaaaaata catcggcgaa ggggtgaagt ttgcaatgca gatacagtca aaccattttg
    21721 tctccctttt caattctata tcctatgttc ttgagacaga cgaaactgaa aatattatta
    21781 acaggataaa agatgttttt cttgacatac atgctgatga ggaatcaaaa aatatcatcg
    21841 aaggcgaaat agcaaagctc catagtcaca ttcagaactc aaaaaatcag atagagcatc
    21901 taagcactat gaattcaagc tattcgacaa taattgatat tgtaaagtcg atagccggaa
    21961 ggaccgattt cagcaggata actgagactg tcgtgaaaaa aatgatagat gccctttctc
    22021 ttgagatatg cggtatagtt tttcttggcg gagactctga agagaacagc tatctgatcc
    22081 ttgactctgt atacaatgag cacaagatga gcgatttccg tttcaaggcg ggggttgtcc
    22141 cgagaatgct gaaaacaggg aggatagatt tcatatcagg cactccttcc gaaaagaact
    22201 ttgaatacag atctgtcgcc gcagttccca taacgctaaa aggtgtaata aaatcataca
    22261 tatacattga aagagactcc gcactgggaa ctttcaggga atcagagata agatttcttg
    22321 aaactcttgc agaaagcatt gctgtaatat ttgacaacat tgaacttatg cagatagcga
    22381 caactgacaa ccttacgcgt ctttattcaa gaagacattt catgaacata ctttcaaagg
    22441 aagttgaaaa agccggcaga tataatttta ctacatccat gataatgctt gacatcgacc
    22501 acttcaaaca ggtaaatgac aactacggcc atctttcagg ggattcgatc ctgagaactc
    22561 tcggggaact ccttaaaaga actgtccgtt cctctgacta tgtgggaaga ttcggaggcg
    22621 aggagttcgt aattcttctg acaggaactg atctcgaagg agcttttcag actgctgaaa
    22681 aaatcaggaa agcgtgtgaa aagcagaatg tttcaggtat ttcgtttacg gtcagtctcg
    22741 gggttgcctc cttccatgaa gacagagtta aaaatgaaaa ggattttata gaaaaatgtg
    22801 atatggctct ttacagggca aaaaatctcg gtagaaacag aactgtcaga tattgtgatc
    22861 ttttagggag ataatcatgg tgcttaacat aaagatattt gaagaacaga tcattcaggg
    22921 agatacttcc cacatcagaa aagagatgga cgctctgctt cagtcatctg ccatgatcaa
    22981 aaagcatgaa gatgatattt ttcttaattt ccaaggactt actccttatg acatattcaa
    23041 gaaactgaaa aatgaacaat acaggacatg gttcaaaaaa cttgatacgg aagtccctgg
    23101 actccccttc tttcttaatg aatcgtcaaa ttcgctcctt tttttcctga tgggaaacat
    23161 ttcatttgaa gaaaagaatt cttctgttct gtttgataag atcgaggcat cccgtttttt
    23221 caaggagaaa atgatccaga tgaggaatct gtgcgttccc cacaacataa accctcagac
    23281 ggggatcacg aaactttcga aagttctttc gggtgaaaca aaaaattccg actccggcca
    23341 aaacagcgac aggcaatctg aaaacagcag cggcagatca atggaaaatg aaaaaaagaa
    23401 aggggccata aaagattttc tcgacagata cgggtcgatc gcatttctca acagcgacag
    23461 gaccgtcaag ctaaccattg tgataaatga gatcccgagc cgcatatcgt tcatcagaaa
    23521 ctttcttgtt aaagacccgg cacttcctca gcagttcttt ctcaccgtcc tgaagaccga
    23581 ttcaggaatt tcagagataa aatccctgct tcttgcctct ataaacgata tagagtcatc
    23641 cattcaacat cataaagggg tgtttattca ggtcgttaca aaatcagaag acggttcata
    23701 ccagatgctt cttgaatctg aaaccatttt tccgattaca gtgacaagtg atctgaaaaa
    23761 cgatattgcc gcaatagaaa gcgaagtatc ggtctcattc ggaacagaga tctcaaaaac
    23821 tgaagcacct gatccgattg aaaaaacgac cgttcctgct gaacagaaaa cgatgataga
    23881 aaaagacgct gaacctgttg aagaaacgga gattatccgt ctcaaaaaag aaaatgaagc
    23941 acttcttgtc aaagttgaaa agcttgaaaa actgatcgag gtttatgaag aggaaattaa
    24001 taaaaaaagg tcaattaaag ggtttttcgg gaagtttttc agttgatgaa aatatttttt
    24061 aacggaaccg gctcttcacc gggtgtgccc cagctcttct gcgactgccg ggtctgcaag
    24121 tcatcattcc gggaaaacaa aagaacccgg ttttcaatga cactaattga aaaggagacc
    24181 atttttcagg ttgatgcccc ttttgaagtt cggcttcagc ttttaaatgc cggtataaag
    24241 aaaattgacg gattgtggct tacacatgcc cacagcgatc acattgcagg cattgatgat
    24301 ctgcggatcg cctctttcag aaacggtgca ccgctcccgc tgtttgcagg acaggaaacc
    24361 atcgaatcag taaagacaag atttccgtat cttttctttg aaaatgaata taccgacatc
    24421 ccgcttcttg aacctgttgc tgttgatgaa aaaccttttc aattcaagga tatggaattc
    24481 atccccgtca ggcattttca cggtgaaaca aaagtccaca gcttccggat gaaagatttc
    24541 ggactccttg cagacatcag ctcaatatca gaaagtgaac ttgcaaaaat gaaaggggtg
    24601 aaagttcttg cagtctgcac aacagtcaac caccctcaca gaaaacatat ggaccttgat
    24661 gaaataatcg aactgataaa gatcatctca cccaaaaaag catatctgac acacatgagc
    24721 cacaattttg actattttga gataaaaaat catctccctg aaaacatctt tcccgctttc
    24781 gacggattaa gggtggaagt ttaaaactta tcaaaaaatt gctttttttt tctatcatcg
    24841 taacatcttt gaagttaata ttttgatcag aggtgagcat gttcaggatt tcagttatta
    24901 ttacagttgc ggttatgata ttcagctgtt cgacggctga aaaaaagacg agcggcagtg
    24961 ttgaaagcgg gaaaacttcg tctgccggga aacatgaact taccgccaag ggaaaaaagc
    25021 ttgagttcga cgcaaagaca tatgttataa aaaaatatga cataaataag gacaaaaagc
    25081 ccgacatcat caatgttttc agaaagttgc ctatcgaagg caaaagaggc gaatacgacc
    25141 tcaagatcca tgttaaaatg atggacctca accgtgacac aaagatagat gtgtggagat
    25201 tttttaacga cagcggtgct gtaataaaag aagagcttga ccttgacttc gacggaaaaa
    25261 tagatgctgt tgaccactat cttaacggaa tagtcagaag aagagaggtt gattttcagt
    25321 ttgatgaagg aactgacatc tggaaacatt ttgatgaaaa aggcgcactg atgctcattg
    25381 aagcggatca atcaggtgac ggaaagatag actactggga acactatacg aacggtgcga
    25441 tcgaaaggat cgaaaaagat accgacgatg acggcaaacc tgatattttc aagagatcgg
    25501 gagatgccga tttcacaaga atattaagca ctaccgataa gtttgaagat cagaacattc
    25561 agaagataga ggaaaaaccg gaagatgaac ttcctgaaca ggtagaggaa cagaaggaag
    25621 aaaaggacgc atctcaggaa gaaccggaaa ccgctccccg gaccggccaa gataaataat
    25681 tcccctcatc attcttgata atctgccggc aatcattatt ataagttgtc tttcaggatg
    25741 tttatttcag gagttgaaca tgaaggttct tgtagtaggt aaaggggcaa gagaacatgc
    25801 tcttgcatgg aagatctcaa aaagccctct cgttaaaaag gtctatgctt tccccggaaa
    25861 cgccggaatt gcgcaagtag ctgaaattcc tgacattaaa agtgacgaca tcattgatct
    25921 cgcttctttt gctttaaaag aaaagatcga tctgaccgtt gtaggacctg aagattatct
    25981 tgcgaaaggg gtcgttgatg tttttcagtt caacggactt aaaatattcg gagctaacaa
    26041 ggcggcggcg gcacttgaat caagcaaggc gtttgcaaaa gagatcatgg attccgcaaa
    26101 aataccgaca gcggcatata caacagtgag tgactttgaa aaaggtgcaa ctgtcttaaa
    26161 aacatcaaaa tatcctgtgg ttttgaaagc cgacggtctt gcggcaggaa agggtgttgt
    26221 gatttgtgac agttatgagc aggcggcaaa agaactttct gaaatgcttt ccggaaagtt
    26281 caaagatgca ggaacaaaag ttgtcatcga ggaatttctg aaaggcgagg aactttcact
    26341 gcttctgctg acagacggaa caaatgtaag aaaactcctt ttttctcagg atcacaaagc
    26401 gcttctggac ggtgacaaag gccctaacac tggcggaatg ggtgcctata caccggtttc
    26461 attcgcggat gatctgcttt atgaaaaagt taaccggttg gtgacattgc cacttctgac
    26521 agaacttaaa aaaagagata tcacatatcg cggagtactt tatatcgggc ttatgatagt
    26581 caacggagaa ccctttgtcc tcgaatacaa cgcaagattc ggagatcccg aaacggaacc
    26641 tctgatgttc atgctcaaaa gtgatattgt cccttatttt atggcttgtg cacaaggaga
    26701 tcttgagaaa ttacatgagc ttgaatggtt tgaaggatat ggagcgacag ttgtcatggc
    26761 ttcaggcgga taccctgaat catacaaaaa aggagaacct attcaaggcc ttgatgtaat
    26821 tgaagaaaat gccgttgtgt tccatgccgg aacaacgatg aaaaacggga atattgttac
    26881 agacggcgga agagttctga tggtgacagc atcaggaaaa acaatacatg aagcgatgaa
    26941 aaatgtttac agcaatactg aaaagatcag ttttaataat tctttcttca ggcacgacat
    27001 aggacacaga gagatcaaca ggaacaaata atgataataa agaccgtcag tttcatcaaa
    27061 agtgcttcgt cagttcagaa tctcccttca acaggtctgc ctgaatttgc tttcatcgga
    27121 aggtcaaatg tcggtaaatc gagcttgatg aacatgctga tggggagaaa aaatcttgtc
    27181 aagacttcaa aaaaaccggg caaaaccgcc cttttgaact attttataat aaatgatctg
    27241 ctctattttg tcgatcttcc gggctacgga tttgcatcaa gatcaaaaag cgagctcgat
    27301 tcgtggagat ctatgatcga ggagtatctt ttaaaaagga cagaactcct tgaaatattt
    27361 cttctcattg attcccgtca cggcattatg gcaaatgatg aacagatgat gacttttctt
    27421 gaacatcacc ggcttaacta tactcctgtt ttcacaaaaa ccgataagat caataaaacc
    27481 gagcttaatg tattgaaaca gaaaaaccgc gattcctttt tctgctctgc tgtaaaaggt
    27541 gacggaaggg aactgcttct tgaccatatt gaaaagataa tcggtaaaaa ggaataatat
    27601 gaaactcttt atccttatta tcattatgtt tttcacattt atctgccatc cttcaaaagt
    27661 aattgtcgaa agtgtcgtgg ctaacatgaa cgacagagcc ataacttatt cagagatact
    27721 tcaggaaggc gaactcctga acattgaaag caatgttgat ccccggacac ctctttccgc
    27781 tgaactcaag gagcggatac tcgagctgat aatgttccgg ataatcgtat ttgaggaagc
    27841 acgaagcgaa ggtgtcacaa ttgatgaatc aaagatccag gaaaaagtga aaacctatct
    27901 ttcaaatgtg tacatgaaag atttcaggaa aacatacagc atctccgacc ttgaattcaa
    27961 cactatgata aagatccggc ttaccgctga caaaatgaca gagatctttc ttgataaaaa
    28021 attcaaagga aagaccccgt caaaagagga aaaacagaaa gctgtgactg agtggcagaa
    28081 aaatctttta aaaaaacaga aacttgtgct atattcgata ccgtaaaaag gaggatatat
    28141 gggaaaagta atatcaatag tgaaccagaa agggggtgta ggcaagacca caactgctgt
    28201 gaaccttgcc gcatgtctcg gtgtcatgga aaagaaagtt ctgctcatcg acatggatcc
    28261 tcagggaaat gcaacatcaa tgagctcggt caagggtgag aatttcaaca caatatatga
    28321 catgatggcc gatccttcgg taaatgcaat ataccccaca gggcttccat ttctaaaaat
    28381 aataccatgt aattcagaac ttacaggtgc agaagttgag ctgcttcaaa tggacgaaag
    28441 agagttcgtt ttacggcaga ctatcgacaa gttaaggaat aaattcgatt ttattataat
    28501 tgattctcct ccgagcttga acattctcac tgtaaataac atggtggctt cagatggtgt
    28561 tatcatcccg atacagtgtg agtattttgc tcttgaaggg cttagcagaa taattgagac
    28621 gatcaaaacc ataaaaaatc tgaacagtga tctcgaaatt tacggaatag tccttacaat
    28681 gtatgataaa agagtgaagc ttacctatga agtggagaac gatgtcaggg aacacttcag
    28741 ggagaccgtt tttaacacag tgatcccaag aaatgtaaaa ctgagcgaat caccaagttt
    28801 cggaatgccg atcgttctgt atgacatctc ttcgatcgga gctaaaaaat acctggaact
    28861 tgccgaagaa ttcctgtcca gatttaaagg gggaaagaaa tgatcgaaaa aagaaaactc
    28921 gggaaaggac tcaatgcact tataaaatcc tcacctgaaa atgattacat ggttctctcg
    28981 atagattcaa taatcaccaa caaggaccag cccagaaaaa ggtttgacga acagcggctg
    29041 acagaacttg ccgcttcgat aaaacaatac ggcatcatcc agcccatcgt tgtaaaaaaa
    29101 gagggggcta aataccggat agtcgcaggt gaaagaagat acagggcggc aaaaatggca
    29161 gggctcacag aaatcaaggc aattcttttc aaaggcgaag aggattatca gatctcgctg
    29221 atcgaaaacc tccagagaga agacctcaat ccgatagaag tcgccgaagc atacaccgaa
    29281 cttataaaaa ggttcagcta tacacagcag gaactcagcg aaaaagtcgg aaaaagcaga
    29341 agtgagataa gcaactgcat gcgtctttta aatcttacag gaaagataca ggaccttgtc
    29401 cgcaacggca caatttctgt cggacaggcg agacctcttg cagtgcttga aaaatcggaa
    29461 caggaagcgg tacttgacaa gatcctgaac aaaaagctta cagccagaca ggttgaaaaa
    29521 atagcttcat caaaacagaa taaaactgct gagaaaaatg cctcgggagg atactgggaa
    29581 cttcagcaga aagaactcgc tgttcataca aaagcaaaag tatcattgaa agaaaaaaag
    29641 accggaatat cggtaacttt tgattttgga accatctctg attttgaaaa gttcagtgca
    29701 atcgtaagaa agctgaacaa atgagatctc ttctcttttt ccttattatc gcggttactt
    29761 cttcaattca ggcacaaaaa aaatttaccg ttgtcataga tgccggacat ggcggcagta
    29821 acaaaggggc aatatccgga gaaattgaag agaaagaact gactttggag cttgctttaa
    29881 aattaaaggc attttttgat aagcacggaa gtgaaaacat ccacgctgtg tttacccgca
    29941 caaatgatac tgaactgccg gtgaagaata gaattgaact gatagaaaga ataaaacccg
    30001 atcttttcat atcgctccat ttcaacagtc agaaatttct ttcaacgaac agaggatttg
    30061 aggtctatta tcctgctgat gtaataaatg aaaaaagtgt cgaaactgca cagaaatacg
    30121 accgggcgaa cagatctttc atttacggca gcatattcaa agagatgttc tttaaagcaa
    30181 atctgcatac cacatggaaa ctccctttca atcttttcac acagaaatat gacctcatga
    30241 tgtttgacga tacaactgcg ccgggactgc ttcttgaaat cgcctacctt acttcacctg
    30301 aagacagggc atgtattgaa aatcctgatt ttgttgccga tattgcctgg tatatttatg
    30361 atgctattgt aaaaattgca gaaaaaagcc ggaggggaaa atgaaaaagc agtggaaaat
    30421 tcttattgtt gacgatgatc tctcaatttc aagaagctat cagaaatatc ttgaatcgga
    30481 aggatatata gccatcacgg caaacaacgg cagagagggt ctgcagaaaa ttgaaaaaga
    30541 ggatcccgac ctcgttcttc tcgatatcaa catgcctgta aagaacggtc ttgaactcct
    30601 tgaagacctt aacagaacaa aagaaataaa aatgccgctg ataataatgc ttactgcata
    30661 cggtgatctt gaagccgcga taagatccac ccgtctgggg gcatatgact ttcttactaa
    30721 acccgtttct cttgaaaaac tccgtctgac tgtaaaaaga gggatcgaaa agatcgctct
    30781 gagtgaacat gttgcacaca taatcgaaca ggatcacgac ggatttaaag ataccataat
    30841 cggtcgcgac tccagaatga tcgatattta caagatcatc ggatcgctgg aaagcaacaa
    30901 ggcgacagtg cttataacag gcgaaagcgg aaccggcaaa gatgtggtgg cgaaagccat
    30961 tcatgaaaac acttcaaaag acgaaccctt tatcgcaata aactgtgcgg ctgtacctga
    31021 gaatctgatc gaaagtgaac ttatgggcta tgtaaaaggt gcttttaccg gtgcgatatc
    31081 aaacagggaa ggcaagcttg aagccgttaa aaagggaact cttgtacttg atgaaatatc
    31141 agaaacacca ctcgaattcc aggcaaaact tctccgtctt ctccaggaaa aggaattcta
    31201 tccggtcgga ggaactgaaa aaaagaggtt caacggaaga ataatcgctt taactaataa
    31261 aaatctcaag gatcttgttg aaaaagggaa gttcagggaa gatcttttct accggcttaa
    31321 tgtcataaat attgttgttc cgcctttacg ggaacacatg aacgatcttg aaatgcttgt
    31381 catacatttt atgaaaaaaa caaactacct tatgcataca gcagtagaaa gcataacccg
    31441 cgaagctatt gattttttaa tgacccatgt ctggcccggc aacataagag aacttgagaa
    31501 tgtgatcact aaagcggcaa taaatgccaa agacaggata ctcacaaaag acagtttcgg
    31561 atttctcggc tccttcaatg aaatagacgg ctcgaatgat aagctcagga ttaatagtga
    31621 taatgaagaa cttcttccct tgaaagacat tgagaaaaaa cacatagaat ttgtattgaa
    31681 acaaactaac tggcacaagg gcaagactgc tgaaatactc ggcataacaa ggcctacact
    31741 tgacaaacgg atagaagagt tcgggctcgc aaaagatgtc tgaaaccggc ttctttctac
    31801 cagtattcct ttttttcgct attgtcattt catccctggg ctggcagata cttatcaaag
    31861 gcgtcggggt cgttgataat ctctgggctt tcggactgct gttctcaaca gtattcatgc
    31921 ttacggcatc aattgtcaca tacttccctt tttcaggaaa tgcttttttc atttcaattc
    31981 ttatatttat gaatttaaca acactttatg tttactcttt ccccaaatca atatggccga
    32041 acatgatctg gtttctgttt atcattacag ccgattttct ttcctcggtg gcgttcctgt
    32101 ggtcaagaga gaactgtcat ctcgcctact ttctcatgcc cgctctgttt tcgtccataa
    32161 acattccgat aatcgtacgg aaggtgaaac atcttgaaaa agtgagtgct aaagcggtgc
    32221 ttatacatgt ttccttcata tcatttcttg ccattctttt ccctattgct gcaggaattt
    32281 catcaatgaa tttcaatccc ctgctatttc ttattctgct cacattcact gttccggtga
    32341 ctctggtcct tttcgggata tcatcatatc ttaacataaa caccggctca ggcatcagca
    32401 gtataaattt tccttttatt atatttttaa gcattcccgg aacagttatt ataaagaaca
    32461 tgttctccct cagggttaca atgcttaatt acatcgatag cgtccatttc actttcggcg
    32521 cacttcttct tctttttgcc gctgtcagta tagccgggat acttatatcc atgttcagca
    32581 catcaattga agggttcatc aacagaaaca gacactttta tcagaaatat cttacaagtt
    32641 acaggactgt gatagaagaa gtgacagatc cggctgagct gttcattctt tttgatcaga
    32701 acatagcttc atggtttaaa gagttcaaat cagttagata tatttttttc aacgatgaat
    32761 tttcagcagg aagagatatt tctttttatg aagaaccttc ttctttcaat atcgagacaa
    32821 cagataaaaa atttcttcac gaaccctatt tcataaaaaa ttcggtggat gttcctgatg
    32881 aaataacaaa gatctcttca aaatacaaag gtaatatttt catccccatt gtttaccgca
    32941 acgaagtgaa cggttttatt gcgattgaca gtcgaaagtt ctctcacagt gctacagttt
    33001 gcgttcacaa tattcttgaa atgacaatga acaggtttga gaaactttcg cttttcacca
    33061 gtgttataga agcagaaaag aaaattgaaa tgctcaaaca ttttcaggaa acaggcaaga
    33121 tggtgtcaat tatcgcccat gaacttagaa gtcctctttc ttcaataatg ttcaatatgg
    33181 aagtgatcaa agattcagtc tataaaaaaa gggatgctga ccccgaatat ctcgacattt
    33241 cgcttcgtga gatccggcgg ctcaacgaaa cagtggaaaa aatgctcaat tacggaagaa
    33301 caataaaact cacaccttcg gaaggtgaat ttaaatcttt ttttacagac attgaccgtc
    33361 ttttcccttc aactcctgaa aagataactt tcattgacaa cacaaacggc gaaagattct
    33421 actttgactg ggatatgctg aaaagtgtga tgatcaacat gataagcaac agtcttcagg
    33481 caattgaagg aagcaacggc atcggaaata taactgtaaa atgttttaaa aggagaaacc
    33541 gcataatcat tgaaataagc gacaccggac cgggaatacc gaaagagatc agaaattcaa
    33601 tatttgaacc cttctttaca acaaggaagg aaggcaacgg actcggactt gcaacaagcg
    33661 agaaaattat aaaactttct ggcggatctg taatactcaa ggaaacatca gaaaaaggaa
    33721 ctgtctttca gattgtgctt ccggtggaat aaattacaga aggatcttca ttaaaaaagg
    33781 gaatgcggca aaagttccga ttgaactgcc tattgaagaa aatagtgata caagaagggc
    33841 tcttgttaaa cggttgctcc accatccttt caatttcaat gtgtcagatg acgcattttc
    33901 aagatctttc actgtcggtt tgacaagata aagttgaaca agtcctgtaa ccattcctgc
    33961 accgatggtc ggattaagac ttgttatcgg tgccgcaata aatcctgcaa gaactgtcag
    34021 agggtggccc agagcaatta tacaaccgag tgcggaaagc acgccgttaa tgagtatcca
    34081 gtatattgcg gcttccattg ttgtgctgct gtcgccgttg atgaatcccc agatgaacat
    34141 gaaaattatt attgcaggta taagccacgg cagaaacttt gtcacttttg acgcaggcgg
    34201 gatgtattca atttcagaaa gatcagatcc ttccgttttt tcgttctgaa gatgacttat
    34261 gattcccgga acatgtgccg cgccaaccac agccacagta gtatttccga gatttttcat
    34321 gatatttacc gcaagatatt tatccctttc atcaaggata actcttttta ccgatggaaa
    34381 ttcatcaccc atttcaccaa tgagctgcat taacatatca ccttttttaa gctcttcaat
    34441 gctttcattt gttacatctt tatcatcact tagaaacagt cctccgagaa atttcgcctt
    34501 ttccgtaaat gtcatcgacc tgatcgtcct tttcaatgtg atcgaaatgt cacggtcggc
    34561 atttacaagc ttcaaacctt tttcttcagc catctgaaca gcttttttga attcagttcc
    34621 gggagcaaca ccggtttttt cagctatctt tttctgataa cttgtaagca tgagctgaac
    34681 tgaaaagaaa agaacctttc cttttctgaa tatctcgaaa atatcagttt cttcatatct
    34741 ttttctgttt ttcagcactt caagccggtt ttcatcaagt tcgactgcaa cagtatcggg
    34801 ggaatattca ttaattgtgt tttccacaag ctgtaccgat tcacctgaaa catgggcagt
    34861 gccaataaga taaaggactt ttcccccttc gaactctatt ttatgaagat tctgacctga
    34921 ctcaccgaca gaaacaaact ttaaactcat gaaaaacctc tgaagttaat gaaccggtct
    34981 gtgctgctcc ctgtgtatga acctcatgac cggagtataa aaaagtgaaa gaaaagcggt
    35041 gtataaaaat ccgaactgga aaagaatgag gaacggcatt gaaaaataaa aacctttctc
    35101 aaatgcaaaa tataccgccc atgaaaagta tatcgcaaaa agaagttcca caatgctgac
    35161 gatactgaca cttgtgaaac ggtacttatt taaagaaact ttcttatatg tttccttccg
    35221 gcaggaaatc tttcccgaaa catcatattt gggagttctg ataaaatccg tctttcttcc
    35281 tgccagggcc tcgataacag ctttgcagtt gtttacagca agcccgatcc cgacagagat
    35341 cgcaaacgga agaaaaagaa gaggggaaag cttagatctg tttttcatcg taagcgtttc
    35401 gtttatgacc gttatttcag aatacagata aaagaacatt actgaaaaag ttgcaaaaac
    35461 aaagacaggc aggtcaaaga tcataacttc gtatatcccg tgaccgatcc tgatgactat
    35521 attcaacggc atcagaaggc agagaaatac catcatgaca taggcaaagt ttgcggcaag
    35581 atggaaaaac gcctccattt taacttttga ggaaagttcc tttgaacgga taattgaagg
    35641 gaaaagcttt ttaaagacct gtatcgaacc ttttgcccat ctgaactgct gacttttgaa
    35701 cgaacttatc tcgacaggaa gttcagcagg cacagtgagt tccggaagat aaacgaactt
    35761 ccaccctttt atctgggctc tgtaggaaag atcaaggtcc tcggtcagag tgtcgtgctg
    35821 ccatccgccg gcatcaacga tcgtcgactt tctccatatt cccgctgtac cgttgaaatt
    35881 gaaaaatctg ccgcttctgt tcctgtatgt gtgctcgatc atgaagtgtc cgtcaagcag
    35941 aatgctctga acttcagtca gtatcgaaaa atcgcggttc aagtggtccc atcttgcctg
    36001 aaccattcct atgccgggat ctgtaaagaa atctatcgtt ctttcaatga aatcttccgg
    36061 tgggaggaag tccgcatcaa ataccgcaat gaattcagca tctgtcaaat tctgaccgta
    36121 ttcaagcgct cctgccttga agccgatcct gttgggtctt ctgatgtgac tgatattaat
    36181 tccgcatgct tttaatcttt cacatgcgag cattgctatc tcaactgttt catcgtttga
    36241 atcatcaaga acctgtatct caaaaagctc tctgggatat ctgatctttg caactgagtt
    36301 tataagccgt tcaacaacat aactttcgtt gtatacagga agctgcacac atactttagg
    36361 cagtttttca aatgttttca gaggtttcgg cacatttgac ttgcactttc tgtaaatgta
    36421 tatcgtgtag tatctgtgaa aaccgaaaat agagagcagc agcagaaccg ccacataact
    36481 gtaaagaaga aagctttcca agttatcacc tatttaaata acactaaaaa aacactacct
    36541 attatcattt aagggttttt tgtcaatatt ttttggaaat tggaacttat tgcatttttc
    36601 atttcagacg gctcaatccc ttttatatgg ctggcctttt cgatcacatt cagtatatct
    36661 tcaagcgttg aaaagtcttt tcccgatact ggctgatatg gggcatcggt ttctgcgaga
    36721 accctttcag caggcaattt atccagtgtt ttcaaggcct tcttgtgccc attcaaaatg
    36781 gcggcaccga aactgaaaaa agcgtttaca tctcttttaa gaaaaaattc cgcctgttca
    36841 aatgtgcctg aaaatgagtg aaaaatgaca gccggaagtt tggaaagttc tgaactgtaa
    36901 cgaaacatat cttcataagc ttttcttatg tgaagagtca caggaaagtt gttttttacc
    36961 gcaacatcaa gctgaaactc aaaaacctcg atctgttttg taaagttttc cttaaactct
    37021 tcatatctgt caaagccgat ctcacctatt gcatctatcc ttttttccag gatcagttgc
    37081 tcaagtatct tcaacttgct ctgatcagga tcctgcggat gaactccgaa agagatgaac
    37141 atcttatcac tgaatttttc tgcgacttta acatcttcca tactgtggca cgatacgatc
    37201 gccctgtaat cactttgatt aatgaaatca ccgtcaggat gaagatctaa aagctgtcta
    37261 agatgcaggt gagcatctgt tatcatggca gatataccgt gaaaggaaga ttctgacggg
    37321 ctataaagga attgaagaac attgaattct ttttcttgaa ctcttcaatc agaccgctta
    37381 gaagatccgg tgaattgttc tcttcgctta agtgactgaa aattattgtc cttggacatt
    37441 ttccgcccat tctgtcaatt atttcaagcg actgatcgtt tgacaggtga ccgtgagacc
    37501 ctcttatcct cgctttgagt tccgccggat actttctgtt tgcataaagc attgaatgat
    37561 cgtggttggc ttcaaggata atgtgatcac atctgctgat ataactcatc attctgttgg
    37621 ttacttttcc tgtatctgtt gcaaaacaga gtgtctgatc cggccccatt ctgatcagaa
    37681 atccgacggg atctgccgca tcgtgcatta tttcaaaagg gatgcagctg aaatttccga
    37741 tgctgaaaga gacatccttg tctatgcact tgtatttgag atcatacttg catgctgaat
    37801 ttattataga gatcgtcttt tccgttgaat agacaggaac accgagctta aaagctgtgt
    37861 aaggaattcc gcaggaatga tcagtatgtt cgtgtgtaag aagtatccct tttatctttg
    37921 aaacatcgac tcccagcccg tctgcccttt tctggaattc cttaaagctc aaaccctgat
    37981 caatgaggat catttctcta tctgattcaa ggataaaggc gtttgccttg gagccactgg
    38041 aaagtatgtg gatcctgttc aatcacatct ccagaaactt tttaaggtct ggaacaaaac
    38101 ttttttcttc tctttcaagc aggaaggtgt tgaaaagtgc ctgcattata agatcaagcc
    38161 gcgtcagtgc ttcaagacgc atggagatca tttcgttttc gtccttttct cccgtgagag
    38221 gaagttttac tgaacttatt ttcaaagggg ttgcccttat ctggaaaaac cattcgagct
    38281 cttttgaaat tatcctcact tttgcgattt cgggaagacg gcctgctttt atcgaatttc
    38341 tcacagacat taactctgcg atatcatgag attttattgt ttcgctgtat ccgtcaccgg
    38401 ttatagaatt gagcgatatt gtctcttcaa gggatatgtg tatttcccca agccccagat
    38461 tttcaagcga taatgatcct tcttcacagc ttttccagta aagccacaga agaaattctg
    38521 acccgaggaa cccgacttct ttaccttttt tcatcattgt acactccaga tctttccaaa
    38581 aacaggttct attttctctc ttgaccccat tctgtcaact gaatcgaaca gcgacaccgc
    38641 ttcaagattt acttcaaaag ttttttcaaa cacatcggta aattttgcaa ttatctgatt
    38701 tgactggctg aaaagataaa ccctgctgtt ttccatatcc catgcaactt cgcatatcga
    38761 tgttttaggg aatgaattga ctttgagttt tcttacaacc tgctctttta tctccttttt
    38821 ctgctgggcg gcaatatatt ccgccccgtt ctctttcatg aacatatatt cggcttcttc
    38881 aagataaggt ctgatctgtg aagcactgaa agaatatttg tcatttctga gtgcgaacac
    38941 aaaaaaacgg tcgtgcatca aagaagagaa gttctgattg tcaaaacaga gaatggcatc
    39001 aacccatcct gttgactcat cccgggtgtc agttgattca agtttcttaa atgagaatct
    39061 ttcgagcgct tttctggttt catcaatatt ttcagggact ttacttgcct taaaaacagt
    39121 gaccgaaaca tttccttttg acagttttgt catatttgac ctccaaaaat caagacttga
    39181 ggattatagt ttcaggggtt gaagagtcaa ggaaatgatc ttaacttaaa tcactcaatt
    39241 ttgttatagc agaaaaatgt gtcgtatgtg cagtcataac cgcattggat ctcgctatca
    39301 ggatagagtg catcgcttac aatatccttg cagaaaacaa atccttctcc tcctgtaccc
    39361 attcctctgt cacactcttc cggattttca ataattccgt ttccgcatat atccttcgtt
    39421 gcaagtgtaa aaaagaactc gtaggacggc tgattaatta caatggtctc tatcagtgta
    39481 tagaacttct gagaagcgac ttttaatgta tactgaccgt aaacgagatc tgtcacatta
    39541 agattctgtg aaccgtcaat atccataaac tgtttcacta tctgtgagtt gtggtccaca
    39601 atatacacat tcagcgtgtc tgaagatgtc agagccgttg agccgccgcc ttccgttctt
    39661 gcatcaaaat taagctgaac gctccctttt tcagtcaatg tctcaatttt attcagctta
    39721 aaatcaatat atattgaatc gcccttattc agaacgactg atcttttctg aggatagtat
    39781 ccttccgcaa atgcttcgat cacataaaaa gaaggattta gattcttcac aatgatcgat
    39841 cctccgttct tattcttcat ggcaatattt cccgggttga gaactattcc ggcattcgtc
    39901 atagtcacag ggctccagtt atgatatatg atgaaaactg gagccctgtg actattccgg
    39961 cattcgtcat agtcacaggg ctccagtttt catcatatat cataacttcg atcgtccctt
    40021 ctgcaggcgg agcatgcaat gcacctgtat aactttcggc atttgtcaaa gaaccggtgt
    40081 taacaatata ctttttctct ttcaactgct tctgaccgtt tgtagtagaa atgaccacca
    40141 ctgatatttc accaaagcaa tcgccgaatg agtcaagacc cttaccttca aaaatggttc
    40201 caagcggtga caaactgtta ccattattgt cacctgcaaa atcagacgga tctacatttt
    40261 tgacagtgaa tgtcttgaca cccatatcgt tcactatctc atacacggga cggttctcaa
    40321 gaccggcgcc ttcgatcagg ttgtatccgt aacctcttat tttaagcgta acaagatcta
    40381 tcgtgtctcc tgctgtaaaa cctgcaggaa caagaacatt caccttttca aaaaattcgg
    40441 tcattgagcc gaacatcgga acctcttctc tcctgccgtc cgacacaact gcaactgtat
    40501 ttgaagaagt attgatgctg tcaaccagtt ttacnnnnnn nnnnnnnnnn nnnnnnnnnn
    40561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    40621 nnnnnnnnnn nnnnccgaca caactgcaac tgtatttgaa gaagtattga tgctgtcaac
    40681 cagttttacc gccttactgc tgctttctat tccgtcaaac atttcgacga ccaccttcct
    40741 tattccttct gaaaaaagaa ctatgtcggg tccattctca tatacatttg aagcatatgt
    40801 cagaatgaga taaagtgttc tgaacaattc atgtttctca ccatccgtca aactttcatc
    40861 gagcgggtgg agaacatttg caaaaacatt ttctatttta cttacggcta tctcctttcc
    40921 gtcaagtgtc acacgacctg cagaattaag tgagtatgca ccaggggtca gcttgcactg
    40981 tgccgtggtc tttcctgaaa gccagacaca aactgtttca ccaggaagaa gcagaccgtt
    41041 aagcactccg aatgaaggac ctttcgcaat gttgacatat ttcagaaggg tcagtgcttc
    41101 ttttttagat acgatatatc tgaaatcaac atcttcagca ttcattttga gaataccttt
    41161 tgccgcggac tttcttatgt ccggaagttt tgtcctgtct tttttcatca aagttgcagg
    41221 cagaagtgaa ctgttcagaa tatcggggaa tattacagac ctgtcaaagc tcatccggct
    41281 cagatacatg tcaaaaatga cattccttat gtcaaaaccg ttaaagactg ccgtatatct
    41341 ttctgttcct gatccccctt ttctcaatga aaatccgctg atcatcgaat caatgaaata
    41401 aaggttctca aaaatgttga aatttctgat gtttccaact tcaacattga atacacggtc
    41461 aatatcgcct tcgatctctc tgatatcatt tctccccgcg aagaatttct tcatttctgc
    41521 ggcaccgtta agtttataaa gctcaattgc cttataaata aagctgtacg gactttcgtc
    41581 tattgaaaaa tcattttccc atctgtccag tatttcagat gttttaacag gaattgacat
    41641 tttccttact gaaaaatcgc tgaaatggct tgtcgccgta acaaggacat ttgacatcgt
    41701 tccgctcatg ataggatcac cgcttgcaat gttcattatc ggatcgcccg atgcagtaaa
    41761 cattatcgga tctccgcttg cagtcagcat tatcgggtcc ccgcttgcct gattcagaac
    41821 agacatttca cccgctccgt caaccctttc aacacccagc attatcggat cgcccgaaaa
    41881 cacagaatca agatctatca ttatgaacat catatcattg agaaaaacag tgcctgacgg
    41941 agagaatgct atctttgaga tctgtgaccc tccgccgctt gacaggagtt tgtatcctat
    42001 ctctaaaact gcggcatcgt ccttttttat ctctgtaccg gccgcaaata ccgcaacgga
    42061 actgtcattg aaccagtatg acatatcttc agacatgtca ctgacatcaa taacgccctc
    42121 tttttgaatc ttgaaaaatg taactgttgt ggatgattca gatatcggaa cagttccagt
    42181 tgaagtcaga agttcggccc gcacaacata ctgacctgca ttatcactcc atatctctcc
    42241 agacccgctt tctgttattt catcaaagac ctgccacttt aaattaaacc ccggtgaaac
    42301 ctcagtgctt ccacctgccg gagcgatctt tacaggagaa gtcacatttt caattataac
    42361 ctcatcctgt tcgggcatga tcttaaaacc attgaaaagc tcaatgaatt cagcatcttc
    42421 atttttttca atatcactta aaacggcctc aatgatttca ctgaccttgc ttttctcagc
    42481 cattcccatc accgattcaa aaccttcatg aatcccagta agctctgaat aatcaagttc
    42541 acccagccat attcctgttc cgactttcat atatgctctg acagattttt ccgcatctct
    42601 gagctttgca catccacctg cagcatgcat ttccataaat gcaagatggg tcagaggatt
    42661 tatttgggct ttgtgaacat cgttctctgc tgtaaaacaa ctgaaaaaat cattactttc
    42721 aacacagtat gtctttgagc tttcgaggac atattcaatc tcaaaattgc ctgaactatc
    42781 catatctgat gtagcaatag ctaaatcagc accacactca taaagaaaaa ctttctcgtc
    42841 tttagtagaa tcgttttcaa tccagacagt tccgtaaaca cctgtaaccg gctttacagt
    42901 atcctcatca gcgatctcat cctcatcagc aggaattacc gggtcgtcag tcaattcatc
    42961 cggctgttca tctgtttcat catcaaagac cgcgtcgtcg atctcttcat ctgaaacagt
    43021 atcgctgtct gagatctgaa tgtcgtcttt atcacttttc ttattgtcgt cacaagatac
    43081 gacaaaaata aagaccgcaa gaacaataaa aaaaaccttg aatttgttaa acatattccc
    43141 ctccggataa attttgttcc ggctgatatt ttatctggtt ttaaaggttt tgcaaggacg
    43201 aaattaactg aagttccccc aaaaattttc agtaaatttc tgcctgatag taaaaactgt
    43261 tttgatctga gaagaattaa tccgtcaacc ttttccccgt tttcggatct tttggattat
    43321 cgggttgtgg tgtgtagtca tcaaacggac ataattcagg gtgatgatcg cagtaacact
    43381 ccatatcacg gagtttcatg gccggctggg tatcgtcctg ccacacaagc cagtgccctt
    43441 caatctcagg ataagacatt ctataagcca tgattccgtc attgtgttca acgggaagtg
    43501 cacagtaact tttctttttt gatattctgt agtaacacac ttttccgtca ccttgcggat
    43561 ctcctgcttc atcatttacc ggattaaaaa cattggtcag cagaatagta tcccctttaa
    43621 cctgaatcac cggcaatgtc tttgttcctg ccgacagtcc cggaatatct atttcttcat
    43681 atttgaacgg tgttttactt atatcgactt ttccgatctt atgggatttt gatgtgtcta
    43741 tgttttcata ataaatcagg ttttcatttt ccttatctat tatgggtgtt gcaatccttt
    43801 ctccttcacg attgagtttt atgcagtcgt cacgactctt tggacttttg ctcaggtcac
    43861 acacataccc cttgaaaccc tgatcataaa atgccaactt atctccgacg agattgggat
    43921 ctccgccggt gtctccttca ccgattactg tccatttcca aactccgatt tttgcataca
    43981 tcattctgtc aggatcacca tcttttattt caacatttat aaatacccat ttgtcattta
    44041 taacgggatt gcctgttata aaacgcacag gttcgttcat tgcgaccgac attttgccac
    44101 tgctgtctga atatatcaaa tacttatatg cactgttttt atcaatcccc t
//
LOCUS       DAOOYB010000003        42614 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_755_length_42614_cov_11.034681, whole genome shotgun sequence.
ACCESSION   DAOOYB010000003 DAOOYB010000000
VERSION     DAOOYB010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 42614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42614
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_755_length_42614_cov_11.034681"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /locus_tag="PL056_00390"
     CDS             complement(<1..839)
                     /locus_tag="PL056_00390"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SO_0444 family Cu/Zn efflux transporter"
                     /protein_id="HPM45501.1"
                     /translation="MKDFLNSLLLVFMEMSPYLLLGFFIAGILNELFKGTKLKKFIGG
                     SNLLSIINASILGVPLPLCSCGVIPTGVSLHKSGASKGSAVSFMISTPQTGVDSILVT
                     WSLLGLPFAILRPVIAFITGVAGGVMTSALEKSATESNKISTDEKKEEDKRSLLQKFY
                     AAMKYAFVDFLDDISLWLFVGLITAALISVIIPEGFFTTYVGNNYLSMIVVLAASVPL
                     YVCATSSVPIAVALMLKGLNPGAALVFLMAGPATNAATVLVIGKTLGRKALFSYLFSI
                     IAGA"
     gene            complement(820..1644)
                     /locus_tag="PL056_00395"
     CDS             complement(820..1644)
                     /locus_tag="PL056_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007039729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HPM45502.1"
                     /translation="MDFLGALINYQFLQNAVFAALLASVLCGISGTFVFVNRITFIAG
                     GIAHAVLGGVGAAVYFGFSPFSGAVAASIIFAFVLGIIKFKASQHEDTVIGALWALGM
                     SAGIIFIYLTPGYSVNLLSYLFGNILLVSTDDIMVLAVLGFAVISVAAVLYRQFKSVS
                     FDLEFSILRGVKGFVVYTMMLVMIALTVVVLMKIVGLILVIAFLSLPAATAAVFTNRI
                     GKIILLSSILSFMFSFSGLFVSYNFNLPTGATITAVAGIIYLLSIISGVYHERFSK"
     gene            complement(1645..2376)
                     /locus_tag="PL056_00400"
     CDS             complement(1645..2376)
                     /locus_tag="PL056_00400"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPM45503.1"
                     /translation="MQNIIEFQKVTFSYGRNEVLKDVDLVVEKGDFFAIVGPNGGGKT
                     TILRLILGLEVPGKGKIKIFGDKPDINTSRAGYVPQFSSHDRMFPISVRDVVSQGLIN
                     RNSFFPFYNKRDLMKVEEMMDLLGIKGRAKDRFGDLSGGLKQRTLIARAIVSDPELLL
                     LDEPVASVDSSIEKDIYDMLVELNRKMTVVMVSHDLGFVSQYVNKVGCVNRTFVCHRT
                     EEMSAEIVDGMYSGKNIMIKHKCGI"
     gene            complement(2360..3274)
                     /locus_tag="PL056_00405"
     CDS             complement(2360..3274)
                     /locus_tag="PL056_00405"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HPM45504.1"
                     /translation="MFRAISLILVALSFFLSSCTSKQPDLNKTGVIKVTVSILPQKFI
                     VEKIGKERVKVNVMVPPAASPETYEPSPQKIMELKDSAIYFLIGMAFEKDAFLKIKDE
                     YKNVVFSPMYEGINLRDPDHGSHHDHGNDHPFKPDGEKDPHIWLDPVNLMKMAENTVS
                     ELMRIDQAHRVSYVQNLYLFKKEVQILHDELVDLFKESSGKGFMIYHPAWGYFADRFG
                     LKQIPVEVEGKEPTAVKMAELTDHMNKNKAKYIFLQPQSSAETVKSFSSQTGVQIRFM
                     DPLKENVLENIKDSAIMIKEGLVSAKHN"
     gene            complement(3353..4984)
                     /locus_tag="PL056_00410"
     CDS             complement(3353..4984)
                     /locus_tag="PL056_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45505.1"
                     /translation="MKKLLGLVFIGMFVFIVSCGDDNKKIVPQDDKDVAGDTDEITDD
                     EGDTGDTGNTGDTGNTGDSGNTGNTGNTGDTGNTGNTGNTGNTGNTGNTGDTGNTGDT
                     GNTGDTGNTGNTGDTGNTGNTGDTADPCAPCDLSNRNCAEVNENWICTGCKDGYYENT
                     EYLCIAEGILGAVCDLTEYAGDDCESGKCVDGVCCNDVCDGLCESCNVSDFYKGTCTP
                     FLAGTDPDDECEAGNTYDCDQNGFCDGLGACQMYDTSTVCQTPVCVGNISKGSAYCDG
                     SGTCDLSNRPEADCEPYTCDIPQGICSGDSCTIDSDCVAGYYCQGSTCVNKKPNGSYC
                     TSNNQCSSNDCTLDDFGTDGFKYCVPNAPAHCAKESSWYIANTTFCVTAGGTTYKKCT
                     PDSWGTAINCTNVAKRGCTPAPWKTKAGFYNADSCADTGSGAVCNADTTCEECTYSRL
                     IKIGTKIVSWPSVYNFGTTVCNTNCRGKTGILNDSICAQESTTSTTHRCVEVEDSKGT
                     LVGICAELLGGGETCTFNEQCKSGSCFKGKCTVAP"
     gene            5234..7825
                     /locus_tag="PL056_00415"
     CDS             5234..7825
                     /locus_tag="PL056_00415"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPM45506.1"
                     /translation="MLKRILFVLLSMLLLFQVSAVEFSKPVLGQQIEIKGREYDTFYY
                     FKGTPQHLKQVYDLISIPFSVNLNSLQHIDFQGIPVTSMIREVVISEGHVKAHRIQLI
                     NADRSNWIDLRNLLAANGIPAWPVVTYYAKDPLVLDGRINIQFSKHMPLEKRDEILKI
                     YGLEVIELGVNDPDFYTVSVSAGKDPFAVANSLVEKDLVRWAQPNWFWHMELKTTTPN
                     DTYYSNQWHLTQILAQYAWDTETGAGKDAKIAIVDSGVDTTHPDLNVLSGYDFISNDS
                     DANPNKAQDDHQGVPHGTSCAGLAGAKTNNSLGVAAACWGCPIIPIRLIGGTYLYPTT
                     IKDALQYGVDQGAWVVSNSWGPQGTDNYGNCISSPADNNQSAAVDYGRINGRGGKGTI
                     FLWAAGNDGCNTSYQGFLKDDDMLTISALESNGSMASYSNFGSEIDVSAGAGNYTVDI
                     QGTYGYNYSTGYDGDNLSDLNYTSVFSGTSAATPVAAGAVALMIAANTSMTFSGIMNC
                     VKASAAKTSKTCSKGGWSTIQPTSTNWLQNAKDHCPCYGFGIVDANAMVNGAKNGTCG
                     ACISTADIDLCFGDGYDRDDDCDGTVDNECDTGGIGKAGDPCSKADDCLNTAATVKCI
                     TDSGWTGGYCSANCTKNSDCYNSNSGVECYDGQCIAKCDYNEVRSGYECISDKILPEG
                     TEVVASCGNDLTEGDEVCDGNLRPCTMIDDSFTGGYANCRDDCRGYDTSTCEGGGGDL
                     CGNSNVDTGEICDSETIACSKLASTPPVGTAKCLKDCSGWDKSGCTEESGDSGNSGDS
                     GNSGDSGNSGNSGNTIAENVCGNGELEYGEQCDDGNTEPFDGCDPNCRLEGVKKKTSG
                     GCSAVLF"
     gene            7865..9502
                     /locus_tag="PL056_00420"
     CDS             7865..9502
                     /locus_tag="PL056_00420"
                     /inference="COORDINATES: protein motif:HMM:NF020678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-N-glycosidase F-related protein"
                     /protein_id="HPM45507.1"
                     /translation="MKENTKIIYFLFFLLILSGCSTSNKDTPDNDTVSDPDNLIFDNE
                     MTDEISDDENPDADEQNNLEEGPYGIEFGNTAGDFTISLETGDWNFAQNRSADENYIF
                     IFYRASNSESTAIWKTDMIRLFDRTPDNTHYFFLVDGTAETFSAKMEQLKKTIEDSLQ
                     IGGIPGIRKKIHLTTKPVREINCWFTEWLEKYSDFFLGIDRFQKIRKAGSFHSWESSS
                     SDPRFEFLYKEAELYNYEYELELFLSENSEKSTKINGLNGVKFPEEGWVKDIFFTADI
                     PSFTKPGRLFILLEQICESQKSCEWDRLQNLFLCDNENSDVCTTEIGRWITTYGRSGK
                     WLTDITPMLSLLKNKGKYKFKFSVAGDQYINYLDLIFIEDESLPEPVDIIPLFNGTVA
                     FDEKYNEHWDNTDIDIPQSSTKVLISAYITGHGNGSEQENCAEFCKFESVFKVNGTSF
                     ITDFSNAGTSRGCFDLVSEGTVPNQYGSWPFGRAGWCPGQDVKLINIDITEKIKKGGL
                     NTFSYSAFLNGENYTPVVTDPSGYRAEIPLSSYLIIY"
     gene            complement(9892..11466)
                     /locus_tag="PL056_00425"
     CDS             complement(9892..11466)
                     /locus_tag="PL056_00425"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HPM45508.1"
                     /translation="MKNFIKLFFLLSISVFLISCGTDDDDCLSNDDCGEGFECNIDTG
                     ECEPKEGGDGAAEIKDEGSSSTDDQDEKNDQDVFEKTDDGGIYVQCTPGDTKECYEGP
                     SGSSGIGICSKGIATCVVDGTDWSECVGQVLPEAEICGDGIDQDCNGEDATAETVIDI
                     DGDGFTYCTGDCCETTWECTTPERVGPGSFEVPSNGIDDNCNGTVDEESSGCDVGLNT
                     DSSDPLDLGKAIDLCLAENESEYGLVSARIVFPNGNEMSDPYSGPDGNGGTTNNPSIA
                     VPGSAYSILPGMGNFVKPTQGTNFLILSTGQAKTKIDNLDTDNLTESSAPEDWYLFHD
                     SKFPDSPNCGLLAESPGENPVRDSVMLELVVRAPNNAAAFSIDINFHSAEFPEYVCKG
                     FNDFFVMLLDSTFTSTDMNLQNPADKNLAMDENKNSVGINLAKSGLFKVCKNKVFPPT
                     NFPGCLDDTELQGTGFEGHGATGWLVSRGNIVPGEEFKIRMAIWDTGDHIYDSLVVLD
                     NFKWYESAQKPGTGEK"
     gene            complement(11502..14027)
                     /locus_tag="PL056_00430"
     CDS             complement(11502..14027)
                     /locus_tag="PL056_00430"
                     /inference="COORDINATES: protein motif:HMM:NF019145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45509.1"
                     /translation="MKKLLVIMVAVFAFATISAATGGMFSKADVKKIDSIPAMVTNVS
                     IPVKTDELSLKKFIKESFYKNFGIGLNVEPQLFKAVDIDGTSIFKFAVTYKGVPVEGT
                     YTVLTVRNGKIDRINNGLGRIDLDVSKLIAPEIAVRSAMKVRNITKMPKKYYTEKVIT
                     KHFGNYVPAYIVRFPAIHLADSRFHVVDAKTGKVLKSGNSTLFADGDETPDEETIDPA
                     TATDMAKIWKFNPSVTPDVIDVELPWVAPYDDADLETEQLGFLTTDKDSEGIRKIKAF
                     NCPNVGGTVSLEEMIGYPINVPLCSPTQLANKLDNGSFIYNDCEDGHEFKEENMTSDK
                     IDRCAEISMYYHASKIYNYLRGLYKDIDSTGKFYLQNNDAERPLNVIGNFQMPDTSDL
                     QALMSGTAKLVAMDNAFFSQDNPSIGDLLSQFGVKGDLLVFGQGTKGDFGFDGDVVYH
                     EFGHATIYTAGIVGMEFTDKYGISNEPGSVHEGIADTFAFLMTDNTCTGEYASEAIVK
                     WAQSQGGMVEMDKEGDFYCMRTALNEYTVFEDFIGEVHWDGQPLLAANWAIYKLIKGS
                     DTDTQIHRDNLTKLVLKTLYSIGSADASFKLWADTFMDEVGKDDNFKGKKSDIEKILN
                     DRNFFEEKRARSANETVKTTYIGAGGTASEDDPMGGGGGGIPISEDGEDINVGPSYLQ
                     FYFDVPENAEKDGLSITASVAADSSSSMIGGIGGGGAPVVEVYYRKGSPVEYVTGEES
                     MKTEVLKDGKVEADGTGSIKNWGIAGVEKGTRYYFQFINTAAAAGVITNIKVASADVK
                     EEVSDEDTDSETVDEEISMEDDLLPDEEQDQKKDKSSGCSLTF"
     gene            14207..16078
                     /locus_tag="PL056_00435"
     CDS             14207..16078
                     /locus_tag="PL056_00435"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HPM45510.1"
                     /translation="MSVYAKKWLTFRNYDSVLFLFISIVMNIFFKSAIGLFLTLNGYG
                     FSIVTPPVSFLFASLDSMLAIGIFFLIDKIPQRKMRLISHHLIIIGAIIFFYANYLIY
                     QYFRTFINWGLICFNGAGSHELASYVLNDLNALILVLSFLAGGGICYLLRKKLITPFF
                     RKQWLIFALLVLNLSIFLFLAQFGRAETGKMIRNPLIELLGSFTKEKLMMKEYTDISG
                     FSAPGALIFGNSDRSSGGEGVLNHNKVQNILVIMVESLSLEMTSLRNPDEKSFKIIEK
                     MKENSIIFDSYRTPFPGTSRSFISANCGTLPGTAQETITNYMPGFKCSSIAEKFNSAG
                     YETGFFASSMFTYDNLSNSDFAGKYKVFKDFLSLKEKYGKGGKFYSYQVKDSDTASEA
                     YDFMSKTVENGRNFFTFVFLYSTHYPYDSPLTTNELKGSVENYRKAQEYVSGVIEELV
                     GKMEKRGFLDNTAVVITADHGEAFGKRTGVTGHGQSLHEEAIRIPLIISMPGLNKGTV
                     SKRNGTHVDLAPTLAYIAGLDSNENWQGQNLLDDTTEEKPAFIFTRSATRLNGIIDGN
                     YKYIHDITENTDTLFDLNSDPDELKNLSLEKPEKTEHYRTISKKWAVCQQKWITAKE"
     gene            16304..17671
                     /locus_tag="PL056_00440"
     CDS             16304..17671
                     /locus_tag="PL056_00440"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HPM45511.1"
                     /translation="MKNTLLVNEIREMLEDGLYEDIRLFVESNPPDVIANFIEALKIN
                     EIIKIFSFIDEEIAAEIYSAFEDEVKNQLTKELSNRELAELLYELPDSMHNQILATLS
                     EEERKTVKDLIKDVDSEEQLKQEQQFAHIPGIIRDREGTIHESIRVYKPVNKKLKQIN
                     TLEPGTLINVVDPDQEEIDFLCKELKIHPDLFDYSLDWDERARIEEDDFNKLVIVRIP
                     YYDETNTDNMYGTIPVGIIFSNGSIVTVCNREVVVIKKMIEKMQKTGCSSLEKDGFIL
                     SVLFDTTQLFLLYLKQISNVINIVQKKIQETSRNEDLIKLLNLEKSLVYFATSLKSNE
                     FMTMKLKKAGIVPPNEKNDDLIEDIITESRQGIEMASIYNNIVSEMMNAFSSIISNKL
                     SNIMKLLTLITIVLTIPLLFSSIYGMNIPLPIQNSPYAFEILMVISLIISAIITLFFK
                     KKDWF"
     gene            17772..18533
                     /locus_tag="PL056_00445"
     CDS             17772..18533
                     /locus_tag="PL056_00445"
                     /inference="COORDINATES: protein motif:HMM:NF016611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPM45512.1"
                     /translation="MAYSKLRGARKPKKKDENSVNIAEHDAFQEQGMKLIDKLLAHPY
                     FILGSVAAIIGIVVIATLISSAVKSKSDKLAFDYASAVETFESESGESTEFDNDIQKY
                     TKAISQFEKVMSEQSGSFNAAASMVYVGKAHQRMNNCEKAVEYFKKARSSGKLAEELL
                     FGVYEGEAICHFDKKEFEKAAEIWKEWLNRKTDIYKDHALYYIGLSYEKSGKDDEALS
                     YYKRLKDEHPDSLLIVKINDKVPAQIETAPKPAEN"
     gene            18533..19657
                     /locus_tag="PL056_00450"
     CDS             18533..19657
                     /locus_tag="PL056_00450"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HPM45513.1"
                     /translation="MQNRSASALFFVVLFLFSSALEAQELMIEWAVPLKKNLVQLNRR
                     EQGGVTIFGKDILVSTRSGDLHLFSDKGSIKKTVTFDGEFFLAPIVVDDGNVMVSVSN
                     SVFMLNSSLELVWSVSGKAPVASNPFVTQEYVMVQFHDNSIYLLDRKTGAIKTAYTSY
                     SEEEVSYMRLPEPLMVDDKYVFGFSSGTIVFFMVRKVGANEELVPYFRFKTSKTTRSF
                     EKKDFFDLLSIVPFKDTVMFSGGEYGGYILEGKVNPLDNMKNLNLLIEKNGKYTGFGE
                     GGIYSFDENGVFVSKIFNSLNYISNLMPVSDFAFASTTGEGSILAYSEGHIHLMSKDY
                     SKVLHSIMIPNGVSAKAAVNDKAVYVISDMGVVYKLKVVK"
     gene            19742..20014
                     /locus_tag="PL056_00455"
     CDS             19742..20014
                     /locus_tag="PL056_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPM45514.1"
                     /translation="MNKSTLINVLAEKSDLSKRNAEQFVNLMIDAMISAMAQEDRIEI
                     RGFGSFVIRHYGSYEGRNPKTGQTIKITDKRLPFFKAGKDLLSRIN"
     gene            20050..22596
                     /locus_tag="PL056_00460"
     CDS             20050..22596
                     /locus_tag="PL056_00460"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HPM45515.1"
                     /translation="MRGFFVTVLPLNYKMSFEAASYFTLNAIQLAKHFISQNDGSEIV
                     LVGKVNESGQVYELKRAAMGNSSSVAAVINAASPGEVLIHNHPNGDVRPSHADIEVAA
                     RAAEKGIGSYIIDNSVTLIFPVVERMMPENTEVEPVNPDDVIRILGENGSLKEAFPDF
                     EFRKPQIHMALEVTEAVNSSLVLSSEAGTGTGKSFAYLVPALLYVSKNKGKKVVISTS
                     TIALEEQLFDKDIPFLIEKLGFNEINTAVLKGRSNYLCKRKYSLFRMENLQTQIIPSH
                     QNRTELIHEIDTWMNQLNDGTRTTMNSSVAHDIWSEICADEFACEKSRCRHFNTCYFF
                     KARRRANFASLILINHHLLMADISMKMSEEDPAGILPQYDILVIDEAHNIFKSAISFL
                     GESVSTQNVIKQLNRLFNPKRSSGLLTKILDSYADPEITQSIEKAVNILSSFIPLFAH
                     SITAELINAAGKDSEGTYELDDAEIRERMNTILEKAVISIAEITGLINPVIKKLREIN
                     ENSLMKTINNEIINSLLTDISGVTGKLDGYAFFYTEFCAGKDVSSNVFWGEKDKNSTL
                     TLTVTPLDIQKILAKQIYDPTPCVILTSATLSTGKGDSGFSFFNRESGLELSKREKRY
                     VNLESCFDYSTQVRAFISTDMPSPVTDKELFDIESIEASKEIVCASNGGALVLFTSIG
                     HREQAAKKMTDLDFDVICQGKFATSKIVKKFREDRNATLLATDTFWEGIDMKGDTLRN
                     LVIVRLPFRFPSHPFVKRYVSKLEERTGQSGFKIYTLPNAILKFKQGFGRLIRTKDDI
                     GTVTVLDKRILEQRYGREFIEAAPPGVKFTPLSSIKVAEKIREFFKKPLL"
     gene            22740..23669
                     /locus_tag="PL056_00465"
     CDS             22740..23669
                     /locus_tag="PL056_00465"
                     /inference="COORDINATES: protein motif:HMM:NF012981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HPM45516.1"
                     /translation="MPGIGLISNPYSRKNRKNPSRLQKLINAAPDKNLVKLPVSYDEM
                     DTAISHFKENEIDILAINGGDGTVHVALCSLMKIYKGKDLPKLAILKGGTMNQTATNL
                     RIKGNTVSIFKRVLEGYAKSDRSLKTKKVSLLRANDRYGFIFGNGAVCSFMDVYHRSG
                     DPSPLVAGKAVLSTVGSIIVRGKIYKEIFTPVSQKVTVDNFCFGHSPYLATLITTIPE
                     LGLGFEVMHRSKVDHTKAHMIAFHEKAKIIMQFHRAWMGLSLPPDIADDTIGKRFVID
                     CEKPFKYTLDGDFYSCSGRLTIETGPVMDIIVE"
     gene            23673..24440
                     /locus_tag="PL056_00470"
     CDS             23673..24440
                     /locus_tag="PL056_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HPM45517.1"
                     /translation="MKYRGFGKTHPGMKRELNEDNYAILDNEGVFVVADGMGGHNAGE
                     VASQIAIETVSEFFKASSQDEDMTWPYKLDPSLSLDANKLMVGIKFANRKIFKSSSSN
                     TSYAGMGTTIVSILASNESGEIYIAHVGDSRCYCHYNKVLSQVTEDHSLLNEYIKAGQ
                     ITKEQAKNFPHKNVIMRALGMKDNVLVDIMKRDAKPGEIYIICSDGLSDMVPDKDIEE
                     IMNRGLSLEETAGVLIDQANANGGKDNITLILVMVEE"
     gene            24460..25191
                     /locus_tag="PL056_00475"
     CDS             24460..25191
                     /locus_tag="PL056_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45518.1"
                     /translation="MKKFCVLTAFAAVVSMLFMFVSCGELEKNENDGEQNDEAATDKS
                     EVPDETVVDEEEVVDDAPAELLYPEEVSATCKKAGDTARNLKFIDDKNEERSLAEWYK
                     PNNESSKLIWLIISTYDCPYCNVEKKDIPKLNGKYEKDGMRTILIMNGLLSAPQPTLE
                     PDKIAKLKDTMILMEGSAADHTYGYFSSSQQTEFRKFINEGYPVNIFIDANTMKIVKH
                     FEGWAEEQEFFDETDAFIESILDIL"
     gene            25194..25748
                     /locus_tag="PL056_00480"
     CDS             25194..25748
                     /locus_tag="PL056_00480"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HPM45519.1"
                     /translation="MKNFTFSLIISAFLVSCATTPVNSETDSDKNSEDQKPAIEQSAG
                     SFSLDLERVDGGTFSFEDIKGKKHLLAAFWATWCEPCKTELKKLAQMYGEFSEHIEFV
                     AVSTDSEDLTDKVNEFAIENAIPFPVLIDPSGNTVASIIPGGDTVPYSIIVDKSGNVI
                     SRHSGYKPGDEESLRKELTELLKK"
     gene            25745..27481
                     /locus_tag="PL056_00485"
     CDS             25745..27481
                     /locus_tag="PL056_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45520.1"
                     /translation="MKLLNFFTLFVLIVTVQILQADEKKEKEKDEVTWKTYSKNKSYT
                     YIDHKAKTGSEEDTFNVYKNESFMEGSYKNFSLGGRKTLIVFTNKDFDPSVAEHDYKY
                     SNELLNHSWEHWLDRIYFKGKWKPVQFTLGDFYESMNRGMAFSMKNDPVYGDNSIRGL
                     NITSSVKGFHLKAFGGRANPQIRDKATFQRMHETDDWLAGMETGYKWKKAEFGIQYGY
                     GNYGKYNLLPTSEGIYKETIDVEKEFHLAGAYISLKNPFPRFNWYAGAVYVPFCRENT
                     LVTTQIGDLEPSTETEKTDQNNASAFYSNALYYFDFGKKKNRLTFKLEGKVYNKFFLN
                     YSRMEDPDFQRRYFAPPTLLPKELQIDNEFDTWAVGARITLNENHYGSKFFIDFVKGD
                     SLNNKDALPASKMSIISEYKEEDFWYFAGGGEKAWNNFSISAGAGYHTVEGKNDDPQF
                     KNSRDWIISYLHLGGHISKFSVKFTNDYYVKDMLINGAHEMDFAHELKTVLDISWNKR
                     YFAALKNTFWKNKPEGDAKWYTGGSLGFNYEPFKIYIFGGLEKGGYTCEGGACRFLPD
                     FKGIKAEVDITL"
     gene            27478..27843
                     /locus_tag="PL056_00490"
     CDS             27478..27843
                     /locus_tag="PL056_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45521.1"
                     /translation="MKIFKKIAGSNIGISGLIILILSIFAIIYTLYAREEAFREFEKF
                     KVEEHCFQGEKADPEWEKMSPEERKLKSDKCMTGLNKLTQIKFGPYIFSFTLIAGIFL
                     LVTSMIKAGKEDDDDNKLS"
     gene            27824..28414
                     /locus_tag="PL056_00495"
     CDS             27824..28414
                     /locus_tag="PL056_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HPM45522.1"
                     /translation="MIINYLDKIPDIKPDSFIFRTSVIIGDTTIGKNSSIWFGTIVRG
                     DVNKIVIGERTNIQDNSTIHVTTSLFPTTIGNEVTIGHNAVIHGSTIENRVLIGMGAV
                     LLDGCHIKQNSIIAAQSVLRNGTKIPEGVLVAGNPAKIKRDLSYDEIEGLAKSAANYV
                     KYSYNYLTYSQSSIYSNDEIKSVIEMLRTNVLGSIQ"
     gene            28411..29973
                     /locus_tag="PL056_00500"
     CDS             28411..29973
                     /locus_tag="PL056_00500"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HPM45523.1"
                     /translation="MRKFVSIVFLLFAVNFISAQGKEQLLTVIFTNDSHGMAWQFDEP
                     NNPGIGGLAAQKTIIDMIRAEVQGKGGNVIILSAGDITLGDPRSNICENMPLINGMNL
                     IGYDALTIGNHEFDFGLEVFHKMKNAAKFPFLSANIYEEGGTKAVGKDYLEKKFDDGL
                     KVAVLGLTTRETEVITGAGLMGNLVMTDPIIEAKTRVPFLRKNNDIVIVLSHLGFYET
                     DKSFDGYYGDNYLPKVVPGIDLIVGGHTQKHLTSPVKIGDTHIVQTEGLGKWVGRFDI
                     YMKDKKIVKTDHKLYPVNLKEKKIKDDKTTYELTGDSYAENKAMLDMLNGFKCTFPTK
                     QIGKIDRDLIGDRELSRTQETELGNIITDVMREKTGADVAFMNSGSIRQGLEKGVITE
                     KDIYSVFPFMDTVFIAELTGYQLQEVLDIFAEKGQGSPGFLQISGIGVKLFKGSALEI
                     TVNGKPLDKKRKYKVAFNSFISGGGDGYTVLKNITAKKDTGYCIPSILVENLKIKGTF
                     AKPAEGRIKIVK"
     gene            30005..30979
                     /locus_tag="PL056_00505"
     CDS             30005..30979
                     /locus_tag="PL056_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HPM45524.1"
                     /translation="MKKVLGMGNALVDILVTLPDEKILASLGLAKGSMQLVDSTFSSR
                     VIERIKHYNPSIMSGGSASNTIHGLSRLGVECSFIGKISEDEFGNDFKKDLLKCSINP
                     KLFKGTLETGRAVALITPDSERTFATYLGSAVTLSSDEITPELFKGYDYFHIEGYLVQ
                     NHDLIEKALKTAKDSGLVTSLDLASFNIVSENLGFLQELVKKYVDIVFANEEEALSFT
                     GTEPVESVRIISEMCRIGVVKTGAKGSLVRCGEDVCAVDSFKTSPVDTTGAGDLYASG
                     FLFGLIHDMPLITCAKYGSLLASRVIQKVGAKIPDSQWPGIIEEMKKI"
     gene            30976..>31286
                     /locus_tag="PL056_00510"
     CDS             30976..>31286
                     /locus_tag="PL056_00510"
                     /inference="COORDINATES: protein motif:HMM:NF013089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HPM45525.1"
                     /translation="MSVSVIFLILAILFNAGANIMMKAGVTKDPVKIEEGVLNFIFSY
                     LNNWQLMTGLAFFGIALVFYTKALEKFNLSIAYPLMTSCGLIIVTLWSIFVFHERLSI
                     V"
     assembly_gap    31287..31386
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            31564..34032
                     /locus_tag="PL056_00515"
     CDS             31564..34032
                     /locus_tag="PL056_00515"
                     /inference="COORDINATES: protein motif:HMM:NF012688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HPM45526.1"
                     /translation="MTDKIAVIDVSSYIFRAFHALPMMTSPDGQPVNSIYGFISMFFK
                     TVDKFKGYSFVAALDSKTPTFRKDYFPEYKANRKEVDPNLKCQFKHIEKMLEALNVTI
                     FSMDGFEADDIIASIAEKNRGSDIVIISSDKDLTQLINESVFLYDSMKEKLIGADAVK
                     EKFGVYPNKMKEFLTLTGDSSDNIPGIPGVGPKTAAALLEEYGSIDNIYNNIGNLKPS
                     LKTKFEENRKLVEVSDFLVTLKSDISIDSSVFRTWEGVNEDLFIEFVTELGFKSFLKK
                     RLPGTETGPAEKEPLIKLSPWTEKEFMPGEGQTYFGVFFNDRFNLGENGHFMEFDIAH
                     IPAETVIYSFDVKNFLNYPFPSKTKFIDLQLAYFCHDSGRHSYDAQDLLQLLNCESEV
                     IDNTGLFFDFLCKLKKKIDRMEDRSFLIENIEMPHLEIISHMEMNGIKVDRRKLVELK
                     NEFLTILSDLSLNIASYTGDDFNPNSPKQLSEILFEKLKLPKGKKTKTGYSTDHNILS
                     SLAELDIHPLPALIIKNREYSKLVSTYLDPMFSMLGEDDRLRTTFIVTHAATGRLASR
                     EPNLQNIPVRTDEGKKIRELFIAEKGKKLISLDYSQIELRILASLSGEDEFIEAFNKG
                     EDIHKRTAAAIFQTIPEMVDANMRRHAKAVNFGIIYGMQAYKLSIDTGVDIGFAKKYI
                     DNYFACYPKIKNFIDSTIRKAVETGFSETLLKRRRYIPELSSPNRNIAKSGERMAVNT
                     VIQGSAADIIKTGTIHVHRMLSEKYPDARILLQIHDELIIEAGGDYENTARDCAAELS
                     KSGELIDVKLDVNYSTGNNWSELK"
     gene            34132..35925
                     /locus_tag="PL056_00520"
     CDS             34132..35925
                     /locus_tag="PL056_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45527.1"
                     /translation="MRHIFLMIAILFSFSLFAQEPSLVPIAPAEEKKEEKKEEKKEEK
                     KEEKKSDYQDLYQQYLNKYNSDQKPAQTTEPAPVQTAPAPAQEPQPAAPVTEQPQEQT
                     APAPNLVPVVEAAPVVEEVKPPKEIVPLKPFEVKPYFALSTFGAMNNAIYFFGKDSLS
                     SSYKFALNNAILDLKGGNKMFDSRILLDFAKGLDTSKVIAINYEHDPSLETDASNPLE
                     LDVTPNQPNTLDIIRDVSFKMTHPTYRKDGFGINLGFQAGKFNMPFGLETLYDHELTF
                     SSNSYMNSVFLGEGFNDLGLTLGADFLLSNEMNLGFKFFLFNGSNETMLDGQDKFQDP
                     AIGFDLRFKFDSKFYTTVAFSFIWGSVYHDYDETVKDGIFDTTNGTYLNQEEEIEADR
                     IEKTATSNDYALNKKNILLSIGADLGYKLNDNISIGLMTQFAFSSRSIFNPQRIDENG
                     NSHIDANQKLNDDGNGDHLRFLRDGSTNFWGTSNYTTWGIFAAPYTKLYMFDIMARIS
                     YNKAPYLYKFLEDSNNSSLAFDLDIVYNFCDYGSLALTYTFVREEINKYDRDYNDKNN
                     LDEYTYIKDIFNHNIIALNLFVNYDFLWEKN"
     gene            complement(35929..37296)
                     /locus_tag="PL056_00525"
     CDS             complement(35929..37296)
                     /locus_tag="PL056_00525"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPM45528.1"
                     /translation="MNRKILVIDDEESLLDSMKLILSRSGYTVLTASNAREALKIIKE
                     LEKEVSLIITDLALPDGFDGIDILDAVKDLETVIPVIMITAYGNVETAVKAMQKGAYN
                     FLTKPVNFKVLLQQAGKAIETSVIIKENKRLKEEIRSINTDRYKIVYVSDSMEKLLKN
                     ASEVAKTEETVLITGESGTGKELLAKHVLKESSRAGRPYVAFNAAAVSESLMESELFG
                     YKKGAFTGAERNSPGYVGAAEGGTLFIDEIGEMPVSLQKKLLRFLQSKEYLPVGSAVP
                     VFSDVRIIAATNKDLEKEVEKGKFREDLYYRLSVFPLTIPPLRERKEDIPELIKHFID
                     KLCRQYKKQPHYPDNETVRELLKQEWRGNVRELENFIARYVLTSGGMVQGNSESEIGH
                     PDKKAQHVPPVLSFRIGEKSMKEIEKEVISAALKYTDGNKIKAAELLDVSQRTIYRKM
                     TEEDL"
     gene            37429..38430
                     /locus_tag="PL056_00530"
     CDS             37429..38430
                     /locus_tag="PL056_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45529.1"
                     /translation="MKYFFMAALIIFCLSGCGETKPENVELTITLSFPEGTTCKSYLA
                     DHFTVTLYNSEQKKTDSQDFPCTPETDALVLYVEKDIYYFTVALKGTDELNKSYGSGT
                     VDLSNGDTEIKIEMEEYQGGITFKWNGSDCENFGIHNLKFTLENDDGYVSSVIWGKEE
                     KIEDFEILCSAEMLEIVNIPAVFYTASAKGFRTEESKTPRIIYSVSDKFKVVSGQDQK
                     IDINEYKEVMVSDIKIIWEFDSKSIASCEDAGVASIIASLVSNSDTLSEETTCDNSFS
                     PFYFYDVSAGKYELLFRGLDSSENTLFENDVNTQEVTVDKGLIGSDIININILLKEK"
     gene            38451..38837
                     /locus_tag="PL056_00535"
     CDS             38451..38837
                     /locus_tag="PL056_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012674737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HPM45530.1"
                     /translation="MLTKKQLDKIKKLLNEELAEIEERNREKVNEMENEGDAETFHGD
                     EADQANYLEHRNRLLRLRDRDRKLINKIRQTLEKIEEGEYGTCESCGCDIGFERLKLR
                     PVASLCIECKQEEEEKEERDKTLKRR"
     gene            38886..39482
                     /locus_tag="PL056_00540"
     CDS             38886..39482
                     /locus_tag="PL056_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45531.1"
                     /translation="MKSLIIVTLLLFSFLTPDVLTKMVDAVYIPDPVIVVEGADDKGT
                     PVILEYEKKTGTLIKHSGDREKGEKSTDFDLFLKIFFFPGEHTSPDTMKKASETIAGL
                     LKAKGIKTENCIVSAIDDGGDPVISIGKEKRFSNSNELAVFKGSYLPSGLTVNEETIV
                     FSEYHKSVLPAAFPGRIEFYSNGNLIKTWMFYRKEHRQ"
     gene            39498..41504
                     /gene="priA"
                     /locus_tag="PL056_00545"
     CDS             39498..41504
                     /gene="priA"
                     /locus_tag="PL056_00545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HPM45532.1"
                     /translation="MTEKFASVLIPRPELSQLSYTIPKNLDIKTGDCVEIELRKTNIW
                     GIVSGISDSPPPEMDGKPLKPVLGKILSSPVFADRIETSLLKWIAEYYLYPFPKIIKQ
                     IFSPLIGPGNTLYGSSDIQKHFSSLCTCFSQEKRELNIHQKRVVDSILKKWGDRDYRP
                     TLVFGITGSGKSEIFAELCKDTIKKGKQVLYLVPEIGLTSKALEHLLNRIGSPGVIIH
                     SFMSKKKRFSSIYCAMSGEAKIIVGTRSSVLYPFFDIGLIVIDEEHDSSYKNLEPPYY
                     NARDFAVMKGAKLKIPVVMGSATPSSDSWYNSLTGKYHIEIIKERANNKPLPEIKMFP
                     YIGDLYLPGDIISKVRESILSGDQSLFFLNRRGFATLALCSECRKTVKCPGCNTALIY
                     HKKKKSLLCHHCNFSRNEFNCSECGSTLKFEGMGIEKLVEAVSEYFPGAEIVSFDRDN
                     LTTLSLFDKAVETISKNKGNIIVGTVMISKGHNFPKLKNVIIKYADYILSFQDSRAAE
                     KCFQLIAQVAGRAGRFDTAGIVYAEAVYPDHYLWKYLKNNDYEGFINEELSWRKKLSL
                     PPYTKMMIIRISGKNEKKVNDTAESLHFYINRSLEETGNGHFILFPVSEPPLSRIKNR
                     FKKNITLIAPKNTADLKKLNKIIVGLPDFKGVSVTFDVDPLNET"
     gene            41504..42562
                     /locus_tag="PL056_00550"
     CDS             41504..42562
                     /locus_tag="PL056_00550"
                     /inference="COORDINATES: protein motif:HMM:NF025042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HPM45533.1"
                     /translation="MFDYLKTIRSVSLDFSQYSTSSNREVIVSVGKSGCFMYNSLIGQ
                     HPEVRNLHRLIIVPSAAEADVELLPDIIFSSHPNITEQSFYACGKLFEFIKENRPEKI
                     TVLLSGGSSALIEKSADPEKTVRTNELLLKSGLNILEMNRIRSENSLIKNGKFAALFP
                     KIIWQIFVMSDIPFKNGEKLVGSMPFFREDLKNTALFKCADSNTLHDQISSFFENVNS
                     IKNFTGTVNELADIIKEFINKKTGNLVISGEPTLKIDHPSPGSGGRMSHLALKLLPLI
                     EKSMKLYALSSDGIDGNSGFAGAVIENVSGNSFNITEIENSLANYDSAAFLNKYNMTL
                     KSGYTGINLNDFVILLRS"
BASE COUNT        13393 a         8808 c         8401 g        11912 t
ORIGIN      
        1 gctccggcaa tgatcgagaa cagataactg aaaagagctt ttctcccgag tgttttacct
       61 atcacaagca ccgttgcggc attagttgca ggtcctgcca tcagaaaaac aagtgccgcc
      121 cccggattaa gccctttcag catgagagca acagctatcg gaacagaact tgttgcacag
      181 acatataaag gaactgatgc agcaagcaca actatcatgc taagatagtt gttccccaca
      241 taagttgtaa aaaaaccttc cggaattatt acagaaataa gtgccgctgt tataagcccg
      301 acaaaaagcc acagcgaaat atcatcaaga aaatcaacaa acgcgtattt cattgcggca
      361 taaaattttt gtaataaaga ccgtttatcc tcttctttct tttcatcagt gctgattttg
      421 ttagactcag ttgcactttt ttcaagtgcg ctggtcatta ctccccctgc gacacctgtt
      481 ataaacgcaa tgaccggcct taatatcgca aaaggcagac cgagcagtga ccatgtgaca
      541 agtatggaat caacacctgt ctgcggagtt gagatcatga aagaaaccgc tgaacccttt
      601 gatgcaccgc ttttgtggag agaaactcct gtcggaatga ctccgcatga acataacgga
      661 agcggaactc cgagaatgga agcgttaata atactaagaa gattgcttcc tccgataaat
      721 tttttcagtt ttgtcccttt gaaaagctcg ttaagtatcc ctgcaataaa aaatccaagc
      781 agaagataag gggacatttc cataaaaaca agaagtagac tatttagaaa atctttcatg
      841 atacacacct gaaataattg ataataagta gataattcct gcaactgctg taattgtcgc
      901 tccagtagga agattgaagt tgtacgaaac aaaaagaccc gaaaaactga acatgaaact
      961 taatatggaa gataataaaa ttattttgcc gatcctgttt gtaaaaactg cggctgttgc
     1021 ggcaggaagg gaaagaaatg cgatcacgag tatcaagccg acaattttca tgagaacaac
     1081 aactgtcaga gcgatcatta caagcatcat tgtgtaaacg acaaaccctt tcactcctct
     1141 taatattgaa aactcaagat caaaacttac cgatttgaac tgtctgtata aaactgcggc
     1201 aacagaaatg accgcaaacc ccagaactgc aagcaccatt atatcatccg ttgaaacaag
     1261 aaggatgttc ccgaaaagat aactgaggag atttactgaa tatcccggtg taagataaat
     1321 aaaaattatt cccgccgaca ttcccagtgc ccacagagcg cctataacag tatcttcatg
     1381 ctgggatgcc ttgaatttta ttattccgag aacaaacgca aaaataatag aggccgcaac
     1441 tgctcctgaa aagggtgaaa aaccgaaata aacagccgct ccgacccctc cgagaactgc
     1501 atgggcaata ccgcctgcaa tgaaagttat cctgttgaca aaaacaaaag ttcctgaaat
     1561 cccgcataag actgaagcaa gcaatgccgc aaaaactgca ttctgcaaaa actgatagtt
     1621 gatcaatgca ccgagaaaat ccatttaaat accgcactta tgttttatca ttatattttt
     1681 tccactatac atcccgtcaa caatttcagc agacatctct tcagttctgt gacagacaaa
     1741 tgttctgttt acacaaccca ccttgttgac atactgagaa acaaaaccga gatcgtggga
     1801 aaccatgacc acagtcattt tcctgttcag ctcgacaagc atatcataaa tgtctttttc
     1861 aatagagctg tcaacgcttg caaccggttc atcaagaaga agaagctccg gatcgctcac
     1921 gatcgctctt gcgatgagcg ttctctgttt gagtcctccc gacagatcac cgaaacggtc
     1981 tttggccctt ccttttatcc ccagcagatc catcatttct tcaactttca tcaaatctct
     2041 tttgttataa aaagggaaaa aagaatttct gttgatcaga ccttgcgaaa caacatccct
     2101 gactgatatg ggaaacatcc tgtcatgact tgaaaactga ggaacatagc ccgctcttga
     2161 agtgttgata tcaggtttgt caccaaatat ctttattttt cctttgccgg gaacttccag
     2221 accgagaatg agccgtaaaa tggttgtctt tcctccaccg ttaggaccga caattgcaaa
     2281 aaaatcacct ttttcaacga caagatcaac atcttttaaa acttcattcc tgccatagga
     2341 aaaagtgacc ttttgaaatt caattatgtt ttgcactaac cagtccctct ttaatcataa
     2401 tagcggaatc cttaatgttt tcaagcacat tttcttttaa tggatccatg aacctgatct
     2461 gtacacccgt ctgggaggaa aaagatttta ccgtctcagc tgaagactgg ggctgaagaa
     2521 aaatatattt tgccttgttt ttattcatat gatccgtcaa ctcagccatt ttaactgctg
     2581 tcggctcttt cccttcaacc tccacaggga tctgcttcaa cccgaatctg tctgcaaagt
     2641 agccccaggc cggatggtaa atcataaaac cttttccact gctttctttg aaaagatcaa
     2701 ccagttcatc atgcaaaatc tgaacttctt ttttaaacag atacaggttc tgcacatatg
     2761 atactctgtg tgcctgatct attctcatca actcggaaac agtgttttca gccattttca
     2821 tcaaattcac aggatcaagc cagatgtgag ggtctttttc gccatccggc ttaaaaggat
     2881 gatcgttccc gtggtcatga tgtgaaccat gatcaggatc tctcaagttg atgccttcgt
     2941 acatcggaga aaacacaaca tttttatatt catcttttat tttgaggaac gcatcttttt
     3001 caaaagccat accaattaaa aaatatatgg ctgaatcttt gagctccata attttctgag
     3061 gtgatggttc ataagtttca ggacttgcgg caggcggtac catcacattt accttgaccc
     3121 tctctttgcc tattttttca actatgaatt tctgcggaag gatgctgact gtcactttta
     3181 taacgccggt tttgtttaaa tccggctgtt ttgaagtgca tgaagaaaga aaaaaactta
     3241 aagcaactaa aattaaagag atagccctga acatttttcc tcctgaaaaa aacttttctg
     3301 atattagcac tgaatcgtca gagagtcaat cggcgtgata ttaaacaaat ctttatggag
     3361 caacagtaca tttgccttta aaacaggagc ccgatttgca ctgctcatta aaagtacatg
     3421 tttctcctcc gccaagcagt tcagcacaga tcccgacaag tgtccctttt gaatcctcaa
     3481 cttctacaca gcggtgagtt gttgatgttg tactctcctg tgcacagatg ctgtcattca
     3541 atattcctgt tttgcctcta cagttagtgt tgcatacggt tgttccgaaa ttgtatacac
     3601 ttggccatga aacaatttta gttccaatct taataagtct ggaataagta cattcttcac
     3661 aagttgtatc tgcgttgcac actgctcctg agccggtatc agcacatgag tcagcattat
     3721 agaatcccgc ctttgttttc catggtgcag gagtacatcc tctttttgca acatttgtgc
     3781 agtttatcgc tgttccccat gaatcgggtg tacatttttt atatgtcgtt ccgcctgctg
     3841 tcacgcagaa agtagtattt gcaatatacc atgaactttc ttttgcacag tgtgccggag
     3901 cattaggaac acagtattta aacccgtctg ttccgaaatc atcaagagta caatcatttg
     3961 atgaacactg attatttgaa gtgcagtaac ttccgttggg ttttttgtta acacaagtgg
     4021 atccttgaca ataatatcca gcaacacagt ctgagtcgat agtgcatgaa tcaccggaac
     4081 atattccctg aggaatatca catgtgtatg gttcacaatc agcttcagga cgattagaca
     4141 aatcacaagt tcctgatccg tcacaatatg ctgagccttt tgatatattt cctacacaaa
     4201 ccggcgtctg acaaactgtt gatgtatcat acatctgaca ggcaccgagt ccgtcacaga
     4261 atccgttctg atcacaatca tatgtgtttc ctgcttcaca ctcatcatca ggatctgttc
     4321 ctgccaggaa aggagtgcat gttcctttat aaaaatcgct aacattacag ctttcgcaaa
     4381 gtccgtcaca gacatcgttg cagcatactc cgtcaacaca ttttccgctt tcacaatcat
     4441 ctcctgcgta ctcagtaaga tcacatactg cccccaatat tccttcagct atgcaaagat
     4501 attccgtatt ctcataatac ccgtccttgc aacctgtaca aatccagttt tcgttaactt
     4561 cggcacaatt tctgttgctg agatcacatg gtgcacaagg atctgcggtg tcgcctgtat
     4621 ttccagtatt tccagtatca ccagtgtttc cagtgtttcc agtgtcacct gtgtttccag
     4681 tgtcacctgt gtttccagtg tcacctgtat ttccagtgtt gccagtgttg ccagtgttgc
     4741 ctgtgttgcc tgtgttgcct gtgtcacctg tattgcctgt gtttccggta tttcctgagt
     4801 caccggtatt tcctgtgtca cctgtattgc cggtatcgcc tgtatcacct tcgtcatcag
     4861 ttatttcatc tgtatctccg gcaacatctt tatcgtcctg cggaactatt tttttgttat
     4921 catccccgca cgaaacaata aaaacaaaca ttccaataaa aaccaaacca agtaatttct
     4981 tcatttcccc ctcctaaata aaataaatgg aacaaatagt atttaatatg ttactttctg
     5041 ccggttattt gtcaaaataa ttttttcatg cttggttttt gcgttaatgt gagtataatt
     5101 tttaccgttg ttggtgttga cttgttcaac tcgatgcatt gcattgtaat aattgaacaa
     5161 aaaagtcagg aatctatttt gctcatattt attgtaattg tgttttttag ttattttaaa
     5221 taaggaggac ttgatgttaa aaaggattct ttttgttcta ttgtcaatgc ttttgctatt
     5281 tcaggtttca gccgtcgaat tctcaaaacc ggttcttgga cagcagatag aaataaaagg
     5341 tcgggaatat gacacattct attacttcaa aggcactccg cagcacctta agcaggttta
     5401 tgatcttata tcaattcctt tttcagtgaa tttgaattca cttcagcaca ttgattttca
     5461 gggaatacct gttacgagca tgatccgcga agtggttata agcgaagggc atgttaaggc
     5521 acacagaatt cagctcatta acgctgacag atcaaactgg atcgatctga ggaaccttct
     5581 tgccgcaaac ggaatacctg cgtggcctgt agttacttat tatgcaaaag atccccttgt
     5641 ccttgacgga agaataaaca ttcagttctc aaaacacatg ccgcttgaaa agagagatga
     5701 gatattaaaa atttacgggc tggaagttat tgaactcgga gtgaatgatc ctgattttta
     5761 tactgtttca gtatcagcag gaaaagaccc ttttgctgtt gccaactctc tggttgaaaa
     5821 agacctcgta aggtgggctc agccaaactg gttctggcac atggagctca agaccaccac
     5881 cccgaacgac acatattaca gcaatcagtg gcatctcact cagatccttg ctcaatatgc
     5941 ctgggacact gaaacaggtg cggggaaaga tgcaaaaata gcaatagtcg acagcggtgt
     6001 tgacacgacc catcctgatc tgaatgttct ttcaggatac gacttcatat caaatgacag
     6061 tgacgctaac cccaacaagg cacaggatga ccatcagggg gttccgcacg gaacatcgtg
     6121 tgccggactt gcaggggcga aaaccaacaa cagtctcggt gtcgccgccg catgctgggg
     6181 atgtccgata attccgatac ggctcatcgg aggaacatat ctctatccca caactataaa
     6241 ggatgctctt caatacggag ttgatcaggg cgcctgggta gtaagcaaca gctggggacc
     6301 tcagggtacc gacaactacg gtaactgcat ttcctcccct gccgataaca accagtctgc
     6361 cgctgtagat tatggaagaa taaacggtcg aggcggaaag ggaacaatct ttctgtgggc
     6421 cgctggaaac gacggatgca acacatccta tcagggattt cttaaggatg atgatatgct
     6481 cacgataagt gctcttgaat caaacggatc aatggcaagc tattccaatt tcggttctga
     6541 aattgatgtc agtgcgggag caggaaatta caccgtcgat attcagggaa cttacggtta
     6601 caactattcg accggatacg acggagacaa tttaagtgat ctcaactata ccagtgtttt
     6661 ttcaggtacg agtgccgcca ctcccgttgc agccggagct gttgcattaa tgatcgcagc
     6721 taacacaagt atgacttttt caggcattat gaactgtgtg aaagcaagtg ccgcaaaaac
     6781 ttcgaaaaca tgttcaaaag gtggctggtc aacaatccag ccgacttcaa caaactggct
     6841 tcagaatgcc aaagatcact gtccatgcta cggtttcggt attgttgatg ccaatgcaat
     6901 ggtcaacggt gcaaagaacg gaacatgcgg tgcatgcatc tcaactgctg atatcgacct
     6961 ctgcttcggt gacggttacg acagggacga tgactgtgac ggaaccgttg acaacgaatg
     7021 cgatacgggc ggtataggaa aagcgggcga tccctgttca aaagctgacg actgtcttaa
     7081 cactgccgcc actgtaaaat gtattaccga ttccggctgg acaggtggat attgttcagc
     7141 gaactgcaca aaaaattcag actgttacaa cagcaattca ggtgttgaat gttatgacgg
     7201 acagtgcata gcaaaatgtg attacaatga agtcaggtcc gggtatgaat gcatttcgga
     7261 taaaattctt ccggaaggaa cagaggtcgt tgcatcatgc gggaacgacc ttactgaagg
     7321 agatgaagtt tgtgacggaa atttaaggcc atgcacaatg atagatgatt ctttcacagg
     7381 aggatacgca aactgccgcg atgactgtcg cggttatgac acatcaactt gtgaaggggg
     7441 cggcggtgat ctttgcggta acagcaatgt tgatacaggt gaaatttgtg acagtgaaac
     7501 aattgcatgc agtaaacttg ccagcacacc tcccgtcggc acagcaaaat gtctgaaaga
     7561 ctgctccggc tgggataaat ccggatgtac tgaagaatcc ggtgattcag gtaactccgg
     7621 tgattccggc aattccggtg attcaggaaa ttcaggcaat tcaggcaata caatagcgga
     7681 aaatgtttgc ggtaacggag agcttgaata cggtgaacag tgtgatgacg gaaacaccga
     7741 accttttgac ggttgtgatc cgaactgccg tcttgaaggt gtaaagaaaa agaccagcgg
     7801 tggctgctct gctgtacttt tttaataaaa ttctcattag aaacaataaa ttcaggtatt
     7861 tatcatgaaa gaaaacacca agatcattta ttttttgttc tttttgctga ttctttccgg
     7921 atgttcaaca tcgaacaaag atactccgga caatgatacc gtttcagatc cggacaattt
     7981 gatctttgat aacgaaatga cagatgagat cagtgatgat gaaaatcctg atgcagatga
     8041 gcaaaacaac cttgaagaag gtccatacgg catagaattc ggcaatacag caggagattt
     8101 taccatctct cttgaaaccg gtgactggaa tttcgctcaa aacagatctg ctgatgaaaa
     8161 ctatattttc atattttaca gagcatctaa ctctgaaagc acagcgatct ggaaaacgga
     8221 catgatcaga ctttttgaca gaactcctga caacactcac tatttcttcc ttgttgacgg
     8281 cactgctgaa acattcagtg caaaaatgga acagctcaag aaaaccattg aggattctct
     8341 tcagatcggt ggaataccag gcatcagaaa aaagattcac ctgaccacaa aaccggtgcg
     8401 tgagataaat tgctggttta cagaatggct tgaaaaatat tctgatttct ttctcggaat
     8461 tgaccgcttt caaaaaatcc gcaaagcagg aagctttcac agttgggaat catcatcctc
     8521 agatccgagg tttgaatttc tgtataagga agcggaactt tacaactacg aatatgaact
     8581 tgaactcttt ctttctgaaa acagtgaaaa aagcacaaag atcaacggct tgaacggagt
     8641 taaatttcct gaagaaggtt gggtgaaaga tattttcttt accgctgaca tcccttcttt
     8701 tacaaaaccc ggtcggcttt tcattctgct tgaacagatc tgcgaatcac agaaatcatg
     8761 tgaatgggac agattacaga accttttctt gtgtgataat gaaaattcag atgtctgcac
     8821 aaccgaaatc ggaagatgga taacaactta cggaagaagc ggaaaatggc tgacagatat
     8881 aacaccgatg ctatctcttc tgaaaaacaa aggaaagtat aagtttaagt tctcagttgc
     8941 aggagatcag tatataaatt accttgacct tatttttatt gaagatgaat cattgccgga
     9001 acctgttgac ataatacctc ttttcaacgg cactgttgcg tttgatgaaa aatataacga
     9061 acactgggat aacactgaca ttgacattcc gcagtcaagc acaaaagttc ttatatccgc
     9121 atatattaca ggacacggca acggctctga acaggaaaac tgtgccgaat tctgtaaatt
     9181 tgaatcggtt ttcaaagtga acggcacttc attcataacc gatttcagta atgcagggac
     9241 ttcgcgcgga tgcttcgatc ttgtaagtga aggaactgtc cccaaccagt atggatcatg
     9301 gcctttcggc agagcgggct ggtgtccggg acaggatgtt aaacttataa acattgatat
     9361 tactgaaaaa ataaaaaaag gtggattaaa tacattttca tattcagcat ttctcaatgg
     9421 tgaaaattat actccggtgg ttacagatcc ttccggctac cgtgcagaaa tacctctttc
     9481 aagttatctt ataatttact gacctttttt actgttaacc aagcttaaca gttggcttta
     9541 actttagtaa acacaatttt tccgatatca gccatattaa cagaaatata acacagttaa
     9601 ctcgtttaag ggattatact gacaaaaaca tcgatcaaca atcaaataat gtttggcacg
     9661 caaattgctt ataatgtatc gtgtgagatc tttttaaatt gaaaaaggga agtagttgtt
     9721 cttcattcat cggtcaaaac tgcggaaacg caatcttaac aacaccaaca ctgtgataac
     9781 ttcaaatcaa ttatcgccat caggcgtcaa tgctgaagat cccaggagca accttccggt
     9841 tattaataat gcccccgttt ttttcggggg catttttatt ttatcagatt tttacttttc
     9901 gccggttccc ggtttctgag cggattcata ccatttgaaa ttgtcaagaa cgacaagact
     9961 gtcataaata tgatcccctg tatcccatat tgccattctg atcttaaact cttcgcccgg
    10021 cacaatatta ccgcgggaaa caagccagcc agttgcccca tgaccttcga atcctgttcc
    10081 ctgaagctca gtatcatcca gacatcctgg aaaatttgtc ggagggaaaa ccttgttttt
    10141 gcatacttta aaaagtcctg attttgcaag attaattcct actgaatttt tgttttcatc
    10201 catcgcgaga tttttgtcag ccggattctg aagattcatg tctgttgaag taaatgtact
    10261 gtcaagaagc attacaaaaa aatcattgaa tcctttacac acatattcag gaaattcagc
    10321 actgtgaaaa ttgatatcta tgctgaatgc cgcagcatta ttaggagccc tgacaacaag
    10381 ttcaagcatt actgaatctc tgacaggatt ttctcccgga ctttcagcaa gaagaccgca
    10441 attcggtgaa tccgggaatt tactgtcatg aaacaggtac cagtcttcag gagctgatga
    10501 ttcagtcaaa ttgtctgtat caaggttatc gatcttcgtt tttgcctgcc cggttgaaag
    10561 aattaaaaaa ttggttccct gagtaggttt cacaaaatta cccattcccg gaagaattga
    10621 ataggcactg ccgggaacag cgatcgaggg gttatttgtt gtcccgccgt taccatccgg
    10681 tcctgaatac ggatcactca tttcattgcc attggggaaa actattctgg cacttacaag
    10741 cccgtattca ctttcatttt ccgccaggca cagatctatt gcctttccaa gatcaagcgg
    10801 gtcgctggag tcagtgttca atcccacatc acatccgctt gactcctcat caactgttcc
    10861 gttacagtta tcatcaattc cattactggg aacttcaaat gatccgggac ccactctttc
    10921 aggagttgtg cattcccatg tagtttcaca acagtcacct gtgcagtatg taaaaccgtc
    10981 gccgtcaata tcaatgaccg tttcagctgt cgcatcttca ccattgcagt cctgatcgat
    11041 cccgtctccg catatctccg cttcaggaag gacctgccca acacactcgc tccagtcagt
    11101 accgtcaaca acgcaagttg ctataccttt actgcaaata cctatacccg atgaaccgct
    11161 cggaccctca tagcattctt ttgtatctcc gggtgtacac tggacatata ttccgccgtc
    11221 atcagttttt tcaaaaacat cctggtcatt cttttcatcc tgatcatctg ttgatgacga
    11281 cccttcatct ttaatctctg cagcaccgtc gccaccttct ttcggttcgc attctcctgt
    11341 atcgatatta cattcaaacc cttcgccgca atcatcattg gacaaacagt catcatcatc
    11401 agttccgcag gaaatgagga acacagatat tgaaagaagg aaaaacagtt taataaaatt
    11461 cttcaaaata ccctccattc agaaaattct tccgcacctc ctcagaatgt taatgaacat
    11521 cctgaagact tgtctttctt ctgatcctgt tcttcatcag gaagaagatc gtcttccatt
    11581 gagatctctt catcaacagt ctcgctgtct gtatcttcat ctgacacttc ctcttttaca
    11641 tcagcagaag caacctttat gtttgttata actcctgcgg cagcggcagt attgatgaac
    11701 tggaaataat atcttgtccc tttctcaact cctgcaattc cccagttctt tatcgaaccg
    11761 gttccgtcag cttcgacttt tccatcctta agaacttcgg tcttcatcga ctcttctccg
    11821 gtcacatatt ccaccggaga accttttctg taataaactt caacgaccgg agctccgcca
    11881 ccgccgattc cgcctatcat tgaactactg ctgtcggcag caactgatgc agtaattgaa
    11941 agtccgtctt tttcagcatt ttccgggaca tcaaaataga actgaaggta gctcggtccg
    12001 acatttatat cttctccatc ctcagatatc gggattccgc cgccgccgcc gcccatcgga
    12061 tcatcttcag atgctgttcc acctgctccg atatatgtcg ttttaactgt ttcgtttgca
    12121 gatcttgctc ttttttcttc aaaaaagttc ctgtcgttca gtattttttc aatatcgctc
    12181 ttttttcctt taaaattatc atcttttccc acttcatcca tgaaagtatc agcccagagc
    12241 ttaaatgatg catcggcact tccgatagaa tagagtgtct taagaacaag ttttgtaaga
    12301 ttgtcgcgat ggatctgagt atccgtatca cttcccttga taagcttata aattgcccag
    12361 tttgcagcaa gaagcggctg accgtcccag tgaacttcac cgataaaatc ttcaaaaaca
    12421 gtgtattcat taagagcagt tctcatgcag tagaagtcac cttccttatc catctctacc
    12481 attccgccct gagactgcgc ccatttcacg atcgcttctg aagcatattc gcctgtgcag
    12541 gtattgtctg tcatcaggaa tgcaaaagta tccgcaatac cttcatgaac agatcccggt
    12601 tcattcgaaa ttccgtattt atcagtaaat tccattccca caatgccagc tgtgtaaatg
    12661 gttgcatgtc caaattcatg gtaaacaaca tccccgtcaa atccgaaatc cccctttgtt
    12721 ccctgtccga aaacaagaag gtctcccttt acaccgaact gactgagaag atcaccgatg
    12781 gaaggattat cctgagaaaa gaaagcgttg tccatcgcaa caagtttagc cgtaccgctc
    12841 attaatgcct gaagatcgct tgtatcaggc atctggaagt taccaatgac atttaacggt
    12901 ctttcggcat cattgttctg aagataaaat tttcccgtgc tgtcaatatc tttataaagt
    12961 ccgcgtagat agttgtagat ctttgaagca tggtaataca ttgatatttc agcacatctg
    13021 tcgattttat cactcgtcat attctcttct ttaaactcat gtccatcctc gcagtcgtta
    13081 tatatgaaac tgccgttatc aagcttgttt gcaagctgcg tcggactgca gagaggaaca
    13141 tttatcggat aacctatcat ttcctcaaga cttaccgtgc cgccaacatt cggacagttg
    13201 aatgccttta tctttcttat accttcgctg tctttatctg ttgtgagaaa accgagctgc
    13261 tcggtttcaa gatcggcatc atcatacgga gcgacccatg gaagctcaac atcaataaca
    13321 tcgggtgtta ctgaagggtt gaacttccag atttttgcca tatctgttgc tgttgccgga
    13381 tcgatagttt cctcatcagg agtttcgtct ccatccgcaa agagtgttga attccctgat
    13441 ttaaggactt ttccggtttt agcatcaaca acatgaaatc tgctgtctgc aagatgtatc
    13501 gcagggaatc tgactatgta tgccggaaca taattaccga aatgttttgt gataactttt
    13561 tcagtgtaat attttttcgg catttttgtg atgtttctca ctttcattgc cgatcttact
    13621 gcaatttcag gagcaatgag ctttgaaaca tcgagatcta tcctgccgag cccgttgttt
    13681 atacggtcaa ttttaccgtt tctgactgta agcactgtgt atgtcccttc tacaggaacc
    13741 cctttgtaag ttacagcaaa cttgaaaatt gaagtaccgt caatatcaac agctttgaaa
    13801 agctgcggtt cgacattcag accgataccg aaatttttgt aaaagctttc cttgatgaat
    13861 tttttaagtg aaagttcatc tgtcttaaca ggaatgctca catttgtaac cattgcggga
    13921 attgaatcga tctttttaac atcggctttg gaaaacattc cgcctgtagc ggcactgatc
    13981 gttgcaaaag caaaaactgc aaccataata accaacaatt tcttcatctt aaccacctcc
    14041 ggtcgaatat ttaaataaaa ggtattaaac aacaacaaag tgaattatat aatcatctat
    14101 aaatatgtca acgccatttt tgattgattt tttcaataaa aaacatcctt ttctgttaca
    14161 aaagcaccgg tttaaacaac tgtttattat ttaattgaga tctgcaatga gcgtttatgc
    14221 aaaaaaatgg cttactttcc gtaattatga ttcagtcctt tttcttttta tctctattgt
    14281 catgaacatt ttctttaagt ccgcgatcgg attgtttctc acattgaacg gttacggctt
    14341 cagcattgta actccgccgg tatcttttct ttttgcatcg cttgattcaa tgcttgcgat
    14401 tggaattttt tttctcattg acaagatccc gcaaagaaag atgcggctta taagccacca
    14461 tctcataatt atcggagcaa tcattttctt ctatgcaaat tatcttattt atcaatattt
    14521 caggacattt ataaactggg ggctgatatg tttcaacggt gccggatcac acgaacttgc
    14581 ctcatatgtc ttaaacgatc ttaatgcttt gatccttgtt ctctcttttc tggcaggagg
    14641 cggaatatgt tatctgttaa gaaaaaaact gataacccca tttttcagaa aacagtggct
    14701 gatatttgca cttcttgttt taaatctttc aatattttta ttccttgcac agttcggcag
    14761 ggctgaaact ggaaaaatga tcagaaatcc gctgatcgag cttcttggaa gttttacaaa
    14821 agaaaaactg atgatgaaag aatataccga tatttccggt ttttcggcac ccggggcttt
    14881 aattttcggg aacagcgacc gcagttccgg tggagaagga gttttaaatc acaacaaagt
    14941 tcaaaacatt ctcgtgataa tggtcgaatc tctttctctg gaaatgactt cacttagaaa
    15001 cccggatgaa aaatctttta aaataattga aaagatgaag gaaaattcaa taatattcga
    15061 ctcatacaga acaccatttc ctggcaccag cagaagtttc atttcggcaa actgtggaac
    15121 gcttcccgga accgcacagg agacgataac caattatatg cctggattta aatgcagttc
    15181 aattgctgaa aaatttaatt ctgccggata tgaaacaggt ttttttgcct cttcaatgtt
    15241 cacatatgac aatctgagca attcagattt cgccggaaaa tataaagttt tcaaagactt
    15301 tctgtcattg aaggaaaaat acggcaaagg aggcaaattc tacagttatc aggtcaaaga
    15361 ttccgacaca gcttcagaag cttatgattt tatgtccaaa actgttgaaa acggccggaa
    15421 tttcttcact tttgtgtttc tttattcaac ccattatcct tatgactcac ctcttacaac
    15481 aaatgaattg aaaggatctg tcgaaaatta cagaaaggca caggaatatg tcagcggtgt
    15541 gatagaggaa cttgtcggaa aaatggagaa acgcggtttt cttgataata cagcagtggt
    15601 cataactgcc gatcacggcg aggcgttcgg caaaagaacc ggtgtcaccg ggcacggaca
    15661 gagtcttcat gaagaggcga ttagaattcc gcttattatc agcatgccgg gattaaataa
    15721 aggaactgtt tcaaaaagga acggaacgca tgtcgatctt gctccgacac ttgcttatat
    15781 tgcaggtctg gacagtaatg aaaactggca ggggcagaac ctacttgatg acacaactga
    15841 agaaaagccg gcattcattt tcacaaggtc agctacacgc cttaacggaa taatcgatgg
    15901 aaattataaa tatattcatg acattacaga aaacacagat acactttttg atctaaacag
    15961 cgaccctgat gagctcaaaa atctctcttt ggaaaaacct gaaaaaacag aacattacag
    16021 aacaatttca aaaaaatggg ctgtttgtca acagaaatgg ataactgcaa aagaatgact
    16081 ttccccctat aaattaaatc ctttcattac ttcatcagcg acattctgat tgatgccacg
    16141 gatggagtca actgtgttca tttattttta cctcttttaa taagaaatca gagaaataac
    16201 gggtacgatc agaagaatta ttgaaaacga tttttacttg caaaaaagta aataataaat
    16261 agtatcaagc cgttttcatc atgtcttttt tcaggaggac tccatgaaaa ataccctctt
    16321 ggtaaatgag atcagagaga tgcttgagga tggactttat gaagacatcc gcctttttgt
    16381 tgaatccaac cctcccgatg tcatagctaa cttcattgaa gctcttaaga tcaacgagat
    16441 cattaagata ttttcgttca ttgatgagga gatagctgct gaaatttatt cggcttttga
    16501 agatgaagta aagaaccagc tgactaaaga actttcaaac agggagcttg ccgaacttct
    16561 ttatgaactt cccgattcca tgcataatca gatacttgca acactttcag aggaagagcg
    16621 gaaaactgtc aaagacctca taaaggatgt agacagcgag gaacagctta aacaggaaca
    16681 acagtttgcc cacatcccag gtataatcag ggacagggaa gggacaattc atgaaagcat
    16741 cagggtatac aaacctgtaa acaaaaaatt aaaacagatc aacaccctcg agccgggaac
    16801 tctgataaat gttgtcgatc ccgatcagga agagatcgat tttctgtgca aagagctgaa
    16861 aatacacccc gatctttttg actattctct tgactgggac gaaagggcaa gaatagaaga
    16921 agacgatttt aacaagctgg tcattgtgag aataccttat tacgatgaga caaacacaga
    16981 caacatgtac ggaacgatac ctgttggaat aatcttttca aacggaagta tcgtaactgt
    17041 ctgcaacaga gaagtcgtgg tcataaagaa aatgatcgaa aaaatgcaga agaccggttg
    17101 cagttcactt gaaaaggacg gtttcattct ttccgttctt tttgacacga ctcagctttt
    17161 ccttctttac ctcaaacaga ttagcaatgt aatcaacata gttcagaaaa aaattcagga
    17221 aacgagccgt aacgaagatc tcataaaact cctgaacctt gaaaaaagtc ttgtatattt
    17281 tgcaacttcc ctcaaatcta atgaattcat gaccatgaaa cttaagaaag ccggcattgt
    17341 ccctccaaat gaaaagaacg atgacctgat cgaagacatc atcacagaaa gcagacaggg
    17401 aatagaaatg gcctcgatct acaacaacat tgtcagcgaa atgatgaatg ctttcagttc
    17461 aattatatcc aataaactca gcaatataat gaaacttctt accctgataa cgatcgttct
    17521 gacaatacct ctgctctttt catccatata cggaatgaac attcctcttc cgattcagaa
    17581 ctctccctat gcatttgaaa tacttatggt gatatctctc ataatttcag caataatcac
    17641 gctgttcttc aaaaagaaag actggttctg aaaaactcag atatggaatt tcccttaaaa
    17701 ttgacatttg atacatattt aaatataaac agcggtgctt gatttttgac tactttattt
    17761 tggaggaaca tatggcatat tcaaagctga gaggggcaag gaaacccaag aaaaaagatg
    17821 aaaactccgt taatattgcg gaacatgacg cttttcagga acagggaatg aagcttattg
    17881 acaaactgct tgctcatcct tatttcattc tgggctctgt cgctgcaata atcggtattg
    17941 ttgtcattgc aacactcatt tcttcagccg ttaaatcaaa aagcgacaaa cttgcattcg
    18001 attacgcttc agctgttgag acttttgaaa gtgaatcggg cgagtccact gagtttgaca
    18061 acgatataca gaaatatacc aaagcgatca gtcaatttga gaaagtgatg tcagaacaga
    18121 gcggttcatt caatgccgcg gcatcaatgg tctatgtagg aaaggcccat cagagaatga
    18181 ataactgcga aaaagcggtc gaatatttta aaaaggcaag atcatccgga aagctcgctg
    18241 aagaacttct tttcggagtt tatgaaggtg aagcgatatg tcattttgac aagaaagagt
    18301 ttgaaaaagc cgctgagatc tggaaagagt ggcttaacag aaaaacagac atctataaag
    18361 atcacgctct ttactacatc ggtctttcgt atgaaaaaag cggaaaggat gatgaagccc
    18421 tttcatatta taaaagattg aaggatgaac atccggacag tcttcttatc gtaaagatca
    18481 acgataaagt tcctgcacag attgaaacag ccccaaaacc ggctgaaaat taatgcagaa
    18541 cagatcggca tccgccctct tttttgttgt actgtttctt ttttcatccg cacttgaagc
    18601 tcaggaactg atgatagaat gggcagttcc cttaaaaaag aaccttgtgc agctcaacag
    18661 aagggaacag ggtggggtca cgatattcgg aaaagacatt ctcgtttcta caagatcagg
    18721 agaccttcat cttttctcag acaaagggtc aatcaaaaaa accgtcacat ttgacggaga
    18781 gttctttctt gcaccaattg ttgttgatga cgggaatgtc atggtctccg tttcaaactc
    18841 agtgttcatg cttaattcat ctctggaact tgtctggagc gtttcaggca aagctcctgt
    18901 ggcatcgaac ccttttgtta cacaggaata tgtaatggtc cagttccatg acaattcgat
    18961 atatcttctt gacagaaaga caggtgccat aaaaactgcc tacaccagtt attctgaaga
    19021 ggaggtttcc tacatgcggc ttccggaacc tcttatggtg gacgataaat atgtcttcgg
    19081 attttcaagc ggaacgatcg tcttttttat ggtgagaaag gtaggggcaa acgaagagct
    19141 cgtcccttat ttcagattca aaacctcaaa aacaacaaga tcttttgaaa aaaaggattt
    19201 ttttgacctt ctttcaatag ttccttttaa agatactgta atgttttcag gcggagagta
    19261 cggtggctat atccttgaag gaaaggtgaa tcctcttgat aatatgaaaa atctgaacct
    19321 cttaattgaa aagaacggaa agtatacagg atttggcgaa gggggaatat actcatttga
    19381 tgaaaacggt gtttttgttt ccaagatatt caattcattg aactatatct caaatctcat
    19441 gccggtttca gattttgcat ttgcatcgac aacgggtgaa gggtccattc ttgcatacag
    19501 cgaaggacat attcatctca tgtcaaaaga ctactccaaa gtgcttcatt caattatgat
    19561 tcctaatgga gtatcagcaa aagcagcggt aaatgacaaa gctgtatatg ttatttcaga
    19621 catgggtgtc gtttacaagc tcaaagtagt aaaatgatag aatttttact tgcaatatga
    19681 aaaaaaatga ttaaagattt ccagtcaggt cgaaaagatc ttttttaact aggaggatta
    19741 gatgaacaaa tcgactttaa ttaatgttct tgcggaaaag agtgaccttt ccaaaagaaa
    19801 tgctgaacag tttgtaaatc ttatgatcga tgcaatgatc agtgcaatgg ctcaggaaga
    19861 ccgcattgaa ataagaggtt tcggcagctt tgttatccgt cattacggct catacgaagg
    19921 aagaaacccg aaaactggac agacaataaa aataaccgac aaaagacttc cttttttcaa
    19981 agcaggaaag gatcttcttt caaggatcaa ctgatccttt aagcatcggg attattcagc
    20041 cacgcctttg tgcgtggttt ttttgtaaca gttctccctt taaattataa aatgtctttt
    20101 gaagctgctt cctacttcac tcttaatgct atccagcttg caaaacattt catatcgcaa
    20161 aatgacggca gcgagatagt gctcgttgga aaagtcaatg aatccgggca ggtatacgaa
    20221 cttaaaagag ccgcaatggg caatagttca tctgtggcgg cagtcatcaa tgccgcatca
    20281 ccgggagagg ttctcattca taatcatcca aacggagatg taagaccgag ccatgccgac
    20341 atagaggttg cggcgagggc ggcggaaaaa gggatcggct catatatcat tgacaattcg
    20401 gtcacactga tatttccagt tgttgaaaga atgatgcccg aaaatacaga agtcgaaccg
    20461 gtaaaccccg acgatgtgat aagaatactc ggtgagaacg gctcattaaa agaagctttc
    20521 cctgattttg agttcagaaa accccagata cacatggcac ttgaagtcac agaagctgta
    20581 aactcatctc tggtactttc ttccgaagca gggacaggaa caggaaaaag ctttgcatat
    20641 cttgtgccgg cactgctcta tgtatccaaa aataaaggga aaaaagttgt aataagtaca
    20701 tcaaccatcg ctctggagga acagcttttt gataaggaca tccctttttt aattgaaaaa
    20761 ctgggattca atgaaataaa tactgctgtt ttaaaaggac ggtcaaacta tctgtgcaaa
    20821 agaaaatatt cacttttcag gatggaaaac cttcagacac agatcattcc gtcacatcag
    20881 aaccggaccg aacttatcca tgagatcgat acatggatga atcagcttaa tgacggtaca
    20941 agaaccacca tgaacagttc cgttgcacat gatatctggt ctgaaatatg tgccgatgaa
    21001 tttgcttgtg aaaaaagcag atgcagacat ttcaatacat gctatttttt caaagcacgc
    21061 agaagggcta attttgcatc tctgatactg ataaatcacc atcttctgat ggcagatatt
    21121 tcaatgaaga tgtctgaaga ggatcctgcc ggaatactgc ctcaatatga tattctggta
    21181 atagacgaag ctcataacat tttcaaaagt gcaataagtt ttctcggtga aagcgtatcc
    21241 acccagaatg taataaaaca gctcaaccgc cttttcaatc cgaaaagatc gtccggactt
    21301 ctgaccaaga tacttgacag ctatgcagat cctgaaataa ctcaatctat tgaaaaagct
    21361 gttaatatcc tttcatcgtt tattcctctt tttgcccact ccattaccgc tgagctaata
    21421 aatgccgcag gaaaagacag cgaagggaca tatgaacttg atgacgcaga aataagggaa
    21481 cggatgaaca ccattcttga aaaagctgtg atctctattg cagaaataac cggactgata
    21541 aatcctgtca taaagaaact cagagagatc aacgaaaatt ccctgatgaa aacaataaat
    21601 aatgagatca tcaactctct tttgacagat ataagcggtg ttaccggaaa acttgacgga
    21661 tatgccttct tttataccga gttctgtgcg ggcaaagatg tctcttcaaa tgttttctgg
    21721 ggagaaaaag ataaaaattc aacacttacg ctgacagtaa cgccgcttga catacagaaa
    21781 attcttgcaa aacagatata tgaccccact ccctgtgtga tacttacatc ggcaactctc
    21841 agcaccggaa aaggagatag cggattttca tttttcaaca gggaaagcgg gctggaactt
    21901 tccaaaaggg aaaaaagata tgtaaacctt gaaagctgtt ttgactacag cactcaggtt
    21961 agggctttca tcagcacgga tatgccgtca cctgttacag acaaggagtt gtttgacatc
    22021 gaaagcattg aggcctcaaa agagatcgtc tgtgcttcaa acggaggcgc actggttctc
    22081 ttcacttcaa taggtcaccg cgaacaggcg gcaaagaaaa tgacagatct cgattttgat
    22141 gtgatatgtc aggggaagtt cgcaacttca aagatagtaa agaaattccg tgaggacagg
    22201 aacgcgacac ttcttgcgac agacactttc tgggaaggga tcgatatgaa aggtgatacc
    22261 ttgagaaatc ttgtaattgt ccgtcttcct ttcagatttc caagccaccc tttcgtaaaa
    22321 agatatgtca gcaaacttga ggagcgaaca gggcagagcg gatttaagat ctatactcta
    22381 cccaacgcca tattgaaatt caaacagggt ttcggacggc tcataagaac aaaggatgac
    22441 atcggaaccg ttactgtcct tgacaaaagg atccttgagc agagatacgg cagagaattc
    22501 attgaagctg caccgccggg agttaaattc acacctcttt cctcaatcaa agtcgctgaa
    22561 aaaataaggg aattctttaa gaagccgttg ctttaactca acttattgtt tttattggtc
    22621 aataaaagct ggtattctgt ttgcatataa atttattgtt ttgtaaattc ctctttattt
    22681 gacaaagagg ggtgcggaaa tataattccg cgttgacttt tcaactttag aaggtaatta
    22741 tgccgggtat tgggcttatt tcaaatccat attcaaggaa aaacagaaaa aatccttcgc
    22801 ggcttcagaa acttattaat gcggcacctg ataaaaacct tgtcaagctc cctgtttcct
    22861 atgatgaaat ggatacagca ataagtcatt tcaaggaaaa tgaaattgat attcttgcaa
    22921 taaacggcgg agacggcact gtacatgtcg ctttgtgttc gctcatgaag atctacaagg
    22981 gaaaagatct tccgaaactt gccatcctca aaggcggaac aatgaaccag acagctacca
    23041 acctcaggat aaaagggaac accgtttcga tatttaaaag agttctggag ggctatgcaa
    23101 aaagcgacag atctttgaaa acaaaaaaag tctcccttct tcgagccaat gacagatacg
    23161 gtttcatttt cggtaacgga gcggtgtgtt ctttcatgga tgtctaccac agaagcggcg
    23221 acccgtcacc tctggttgca ggcaaagcgg ttctttcaac agtcggatca ataattgtca
    23281 ggggcaaaat ttataaagag atctttactc cggtcagcca gaaagtgacc gttgacaatt
    23341 tctgtttcgg gcactcaccg tatcttgcaa ctcttataac aacaattcct gaactgggac
    23401 ttggatttga ggtaatgcac agatcaaagg tcgatcacac aaaggcacac atgatcgctt
    23461 ttcacgaaaa agccaagata ataatgcagt ttcacagggc ctggatgggg ctctctcttc
    23521 ctccggacat tgctgatgac acgattggaa aaagattcgt cattgactgt gaaaaacctt
    23581 ttaaatatac tcttgacggg gatttttatt cctgttcagg cagacttaca atagaaacag
    23641 gtcctgttat ggacattatt gtggagtgac agatgaagta cagaggattt ggaaaaaccc
    23701 atccggggat gaaaagagaa ctcaatgaag acaattacgc aattctcgat aacgagggtg
    23761 tttttgtcgt tgccgacgga atggggggac acaatgccgg agaagtcgct tcccagatag
    23821 ctatagaaac ggtttcagaa ttcttcaagg catcttcaca ggacgaggac atgacatggc
    23881 catacaagct tgatccttca ctttcacttg atgccaacaa acttatggtc ggaataaaat
    23941 ttgccaacag aaagatcttt aaaagttcct cttcgaacac ctcttacgcc ggaatgggca
    24001 cgaccattgt ttcaatcctt gcaagcaatg agagcggtga gatttacatc gcccatgtcg
    24061 gtgacagcag gtgttactgc cattacaaca aggttctttc gcaggttaca gaagaccact
    24121 cacttcttaa cgaatatata aaagcggggc agatcaccaa agaacaggca aaaaactttc
    24181 ctcataagaa tgtcattatg agagcgctag gaatgaaaga caatgttctt gtagacatta
    24241 tgaaaagaga tgcgaaaccg ggtgagatat acatcatctg ctcagacgga ctttcagaca
    24301 tggtgcccga taaagatatc gaagagatca tgaaccgcgg actttcactc gaggaaacag
    24361 ctggtgtact tattgatcag gcaaatgcca acggcggcaa agacaatatt actttgatac
    24421 ttgttatggt tgaagaataa acttatttgg agaaaagaaa tgaaaaaatt ctgcgtatta
    24481 acagctttcg cggctgttgt ttcgatgttg ttcatgtttg tatcgtgcgg tgaacttgaa
    24541 aaaaatgaaa acgatggtga acagaatgat gaagctgcaa ctgacaaatc tgaagtccct
    24601 gatgaaacag tagttgacga agaggaagtc gttgatgacg ctcccgcgga acttttatat
    24661 ccggaagaag tatcagcaac ctgtaaaaaa gccggtgata ctgcaagaaa tctaaaattc
    24721 attgatgata aaaatgagga gcggtctctt gctgaatggt ataaacccaa caatgaatct
    24781 tcaaaactca tctggcttat aatcagtact tatgactgtc cttactgcaa tgttgaaaag
    24841 aaagatattc ccaaactgaa cggtaaatat gaaaaagacg gaatgaggac gatcctcata
    24901 atgaacggac tgctttcagc tccccagccg actcttgaac cggataaaat tgcaaaactc
    24961 aaagatacaa tgatacttat ggaaggttct gccgccgatc atacttacgg atatttttca
    25021 tcttcacagc agaccgaatt cagaaaattc ataaacgaag gataccctgt caatattttt
    25081 attgatgcaa acaccatgaa gatcgtaaaa cattttgaag gatgggctga agaacaggaa
    25141 ttctttgatg aaacagatgc gttcatagaa tccattctgg acattctctg acaatgaaga
    25201 acttcacctt ttctctcata atttcagcat tccttgtttc ctgtgccacc acaccggtaa
    25261 actcagaaac agactcagac aaaaattcag aagatcagaa accagccatt gagcagagtg
    25321 cggggtcttt ttctcttgac ctggaaagag tcgacggagg aactttcagt tttgaagata
    25381 tcaagggaaa aaaacacctg cttgccgcgt tctgggcaac ctggtgtgaa ccctgcaaaa
    25441 ctgaactcaa gaaacttgct caaatgtatg gggaattttc agaacatatt gaatttgttg
    25501 ccgtttcaac tgattctgaa gatctgacag ataaagtcaa tgagtttgca atagaaaatg
    25561 caatcccctt ccccgttctc attgatcctt ccggaaacac cgtcgcatca ataattccgg
    25621 gaggagacac cgtcccctac tcgattattg ttgacaagtc aggcaatgtc atttcaagac
    25681 acagcggata taaaccggga gacgaggaaa gtttaagaaa agaacttaca gagcttctta
    25741 aaaaatgaaa ctgctgaatt tttttacatt attcgttctt attgtcaccg ttcagattct
    25801 tcaagccgac gaaaaaaaag aaaaagagaa agacgaggtg acatggaaaa catactcaaa
    25861 aaacaagtcc tacacatata tcgaccataa ggccaagacc ggttcagaag aagatacttt
    25921 caatgtttac aagaatgaaa gctttatgga gggaagttat aaaaactttt ctctgggcgg
    25981 aagaaaaacc cttattgtct tcacaaacaa ggattttgat ccgtcagttg cagaacacga
    26041 ttataaatac agcaatgaac ttttaaatca cagctgggaa cactggcttg acagaatata
    26101 ctttaaagga aaatggaaac cggtccagtt cacactcggt gacttttatg aaagtatgaa
    26161 ccgcggaatg gcgttttcaa tgaaaaacga cccggtttac ggtgataatt caattcgcgg
    26221 gctcaacatt acttcatcag taaaaggatt tcacttaaaa gcgttcgggg gccgcgccaa
    26281 cccacagatc agggacaagg caacatttca gagaatgcat gagaccgatg actggcttgc
    26341 cgggatggaa accggctaca aatggaaaaa agccgaattc gggattcagt acggatacgg
    26401 taactacggc aaatataatc ttctgcccac cagtgaagga atatacaaag aaacaattga
    26461 tgtcgagaag gaatttcatc ttgccggagc atatatatct ctcaaaaatc ccttcccccg
    26521 attcaactgg tatgccggag ctgtttatgt ccctttctgt cgtgaaaata cgcttgtaac
    26581 aacccagatc ggtgatctgg agccgtcaac tgaaacagaa aagaccgatc agaataacgc
    26641 aagtgcattc tattccaacg cactttatta ttttgatttc ggaaagaaaa agaaccggct
    26701 cacattcaag cttgaaggaa aggtctataa caagtttttt cttaattact caagaatgga
    26761 agatcccgat ttccagagaa gatattttgc cccacccact ctgcttccga aagaacttca
    26821 gatagataat gaatttgaca catgggctgt cggggcaagg atcacattga atgaaaatca
    26881 ttacggctct aagttcttta ttgactttgt caagggtgat tcgctaaaca acaaagatgc
    26941 actcccggct tctaaaatgt cgatcatcag tgagtacaaa gaagaagatt tctggtactt
    27001 tgccggaggc ggagaaaaag catggaataa cttctcaatt tcagcaggag cagggtatca
    27061 cactgttgag ggaaagaatg atgatcctca atttaagaac agcagagact ggataatttc
    27121 ttatctccat cttggcggac atatatcaaa attctcagtt aagttcacca atgactacta
    27181 tgtaaaagac atgttgatta acggtgcaca tgaaatggat tttgcccatg aactcaaaac
    27241 ggttctcgac atttcgtgga acaagagata ttttgcagct ttgaaaaaca ctttctggaa
    27301 aaacaaaccg gaaggagatg cgaaatggta caccggagga tcgctcggat ttaattatga
    27361 accgtttaaa atatatatct tcggaggatt ggagaaaggc ggatatacat gcgaaggcgg
    27421 agcatgccgg tttcttcctg atttcaaggg aatcaaagcg gaggttgata taactttatg
    27481 aaaatattca agaaaattgc cggatcaaat atcggcatat cagggttgat aattttaatt
    27541 ctcagcattt ttgcaataat ttatactctc tatgcaagag aagaggcctt cagggaattt
    27601 gaaaaattca aagttgaaga acactgtttt cagggtgaga aagctgatcc tgaatgggaa
    27661 aaaatgagcc ccgaagaaag aaaacttaag tcagacaaat gtatgaccgg gcttaacaaa
    27721 ttgacacaga taaagttcgg accctacatt ttttccttca ctctcatagc ggggatattt
    27781 cttcttgtga catccatgat caaagccggt aaagaggacg atgatgataa taaactatct
    27841 tgataaaata cccgacataa aaccggattc attcattttc aggacatcgg tgatcatcgg
    27901 ggacacaact atcggaaaaa attcgagtat ctggtttgga acaattgtca ggggcgatgt
    27961 aaacaaaatt gttataggtg aaaggacaaa tattcaggat aattcaacaa tacatgttac
    28021 aacctccttg tttcccacca cgatcggaaa cgaagttaca atcggacata atgcagtcat
    28081 tcacggttcg accatagaaa acagggtcct tatcggaatg ggagccgtcc ttcttgacgg
    28141 atgtcatatc aaacagaatt ctataattgc cgcccaatct gttttgagaa acggaacaaa
    28201 gataccggaa ggggttcttg tcgcgggcaa tccggcaaaa attaaaagag atctttctta
    28261 tgatgaaatc gaagggctgg caaaatctgc cgcaaactat gtaaaatatt catacaacta
    28321 tttgacatat tctcaatctt cgatatattc taatgacgaa attaaatcgg tcatcgaaat
    28381 gttaaggacc aatgttttag ggagcataca atgaggaaat ttgtttcgat cgtattttta
    28441 ctttttgcag taaattttat cagtgcccag ggtaaagagc agcttctgac agtcattttc
    28501 accaatgaca gtcacggcat ggcgtggcag ttcgacgaac cgaacaatcc tggaatcggc
    28561 ggacttgcgg cacagaaaac aattattgac atgataaggg ccgaagttca gggcaaaggg
    28621 ggaaatgtta tcatcctgag tgcaggagat ataactctcg gggatcccag aagtaatatc
    28681 tgcgagaaca tgcctttaat aaacggaatg aaccttatcg gctatgatgc tctgacaata
    28741 gggaaccatg aatttgactt cggtctggaa gttttccaca aaatgaaaaa tgctgcaaaa
    28801 tttccattcc tgagcgccaa tatttatgaa gaaggtggaa ctaaagcagt cggaaaagat
    28861 tatcttgaga aaaaattcga tgacggcctg aaagttgccg ttctcggtct tacaacaagg
    28921 gaaacagaag ttataacagg tgccggactt atgggcaacc ttgtaatgac agaccctata
    28981 attgaagcta aaaccagagt tccattcctc cggaagaaca acgacatcgt gatagttctc
    29041 agccatctcg gtttttatga aacagacaaa agctttgacg gttactacgg tgacaactat
    29101 ctgcccaaag tcgttcccgg aatagacctc attgttggcg gacacactca gaaacacctt
    29161 acaagtccgg taaagatcgg tgatacccac atcgtacaga cagaaggact cggcaaatgg
    29221 gtcggccgtt tcgatattta catgaaagac aaaaagatcg ttaaaaccga ccacaagctc
    29281 tatcctgtaa atcttaaaga aaaaaagatc aaagatgaca aaacgacata tgaactcaca
    29341 ggagattctt atgccgaaaa taaggccatg ctggatatgc tcaacgggtt taaatgcact
    29401 ttcccgacaa aacagatcgg caagatcgac agagacctta tcggtgacag agaactttca
    29461 agaacacagg aaactgaact cggcaatatc attacagatg ttatgagaga aaaaaccggt
    29521 gccgatgttg ctttcatgaa ttcgggaagc ataaggcagg gacttgaaaa gggcgttatt
    29581 actgaaaaag atatttatag cgttttccct ttcatggaca ccgtctttat tgctgaactt
    29641 acaggatacc agcttcagga agttcttgat atttttgcag aaaagggtca aggatctcca
    29701 ggattcctcc agatctcggg aataggtgtc aagcttttta agggatccgc tcttgaaata
    29761 actgtgaacg gcaaacctct tgacaagaaa agaaaatata aagttgcatt caactctttc
    29821 atttcgggcg gaggagacgg atatacagta ctcaagaaca taactgcaaa aaaagataca
    29881 ggatactgca ttccttcgat tcttgttgaa aatcttaaaa ttaaagggac ttttgcaaaa
    29941 cctgcagaag gccgtattaa aattgttaaa taatcttctc caaatctttc tctaaggaca
    30001 cgccatgaaa aaagttctcg gcatgggaaa tgctctagtt gacattcttg ttactcttcc
    30061 tgatgagaaa atacttgcat ctctgggact tgcaaaaggg agcatgcagc ttgtggacag
    30121 cactttcagt tcaagggtga tcgagcgtat taagcactac aacccctcaa taatgagtgg
    30181 cggaagtgct tcaaacacta ttcacggact gtcacggctg ggtgttgagt gctcctttat
    30241 cggaaagatc tctgaggatg aattcggaaa tgatttcaaa aaagatcttc tgaaatgctc
    30301 cataaatccc aaacttttta aaggcactct ggaaacaggc agagcggtag cgcttattac
    30361 tcctgattca gagagaacct ttgctacata tctgggttcg gctgtaacac tctcttctga
    30421 tgaaataacc cccgaacttt tcaaaggata cgattatttt catattgaag gatacctcgt
    30481 acagaatcac gatcttattg aaaaagccct caaaaccgct aaagattcag gacttgtgac
    30541 atctctggat cttgcaagtt ttaatattgt ttctgaaaat ctcggatttt tgcaggaact
    30601 tgtaaaaaaa tatgttgaca tagtttttgc aaatgaagag gaagcacttt ccttcacagg
    30661 tacagaacct gttgaaagtg tcagaattat atctgaaatg tgcagaatag gcgttgtgaa
    30721 aacaggagcc aagggatctc tggtccgttg tggagaggat gtgtgtgctg ttgacagttt
    30781 taaaacatct ccggttgaca caacaggcgc aggagacctt tatgcatcag gattcctttt
    30841 cggattaatt catgacatgc cgcttattac atgcgctaaa tacggatctc tacttgcatc
    30901 aagggtaata cagaaagtag gtgccaaaat accagacagt caatggccgg gaataattga
    30961 ggaaatgaaa aagatatgag cgtttctgtc atttttctaa ttcttgccat cctttttaac
    31021 gcaggtgcca atatcatgat gaaagcaggc gtgacaaaag atcctgtaaa aattgaggag
    31081 ggcgttctca atttcatttt cagttacctc aataactggc agcttatgac aggacttgca
    31141 ttcttcggta ttgctctcgt tttttataca aaggcactcg aaaaattcaa tctttccatt
    31201 gcatatcctc ttatgacatc atgcggactg atcattgtca ctttgtggtc aatatttgta
    31261 tttcatgaaa gattgagtat cgttcannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    31321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    31381 nnnnnnctga agtttgacga tatatttcag ggtaaggcag gaacaattaa actcatgcct
    31441 ggcgacagag tatttgtatc tccgacagca cttacaagct ataacaggat aattcaacag
    31501 cttctgccca cattccagat gctttataca ggcacgcttc tctacagaaa ctggaccaga
    31561 taaatgacgg acaaaattgc cgtaatcgat gtaagttcat atattttcag ggcttttcac
    31621 gctcttccaa tgatgacttc tcctgatgga cagccggtaa attcaattta cggttttatt
    31681 tccatgttct ttaaaactgt agacaaattt aaaggatatt cgtttgttgc ggcactcgac
    31741 agcaaaactc cgactttcag aaaggattat tttcctgaat acaaggccaa cagaaaagag
    31801 gttgacccta atctgaaatg tcagttcaaa catattgaaa aaatgcttga agcgctcaat
    31861 gtcacaattt tctcaatgga cggctttgaa gcagacgata tcattgcttc catagcagaa
    31921 aaaaatcgcg gatccgatat cgtgataatt tcatcagaca aagaccttac gcagcttata
    31981 aatgaaagtg tcttccttta tgacagcatg aaggaaaagc ttatcggggc tgacgcagtt
    32041 aaagagaaat tcggagtata tccaaataaa atgaaagaat ttctgaccct taccggcgac
    32101 agctctgaca acatacccgg cataccggga gtcggaccta aaactgccgc ggctctgctt
    32161 gaagaatacg gctcaattga caacatatat aataatatcg gcaacttaaa gccatcgcta
    32221 aaaacaaagt ttgaagagaa tagaaaactg gttgaagttt cagattttct tgtgactctc
    32281 aaatcagaca tatcaattga ctcttcagtt tttagaacat gggaaggagt aaatgaagat
    32341 cttttcatcg aatttgttac agaactcgga tttaaatctt ttttaaagaa acggcttccc
    32401 gggacagaaa ccggccctgc tgaaaaagaa cctttaataa aactttctcc ctggacagaa
    32461 aaggaattta tgccgggcga gggacagaca tatttcggag tttttttcaa cgaccggttc
    32521 aatcttggag aaaacggtca ctttatggaa ttcgatatcg ctcatattcc cgctgaaact
    32581 gttatatatt cttttgatgt aaaaaacttt cttaactacc cgttcccttc aaaaacaaaa
    32641 tttattgatc tgcagcttgc atatttctgt cacgacagcg gaagacactc atatgatgca
    32701 caagatcttt tacagctttt gaattgtgaa tcggaagtta ttgataacac cggccttttc
    32761 tttgattttc tctgcaagct caaaaaaaag atagacagaa tggaagatcg cagttttctg
    32821 atcgagaata ttgaaatgcc tcatctcgaa atcataagcc atatggaaat gaacggaata
    32881 aaagtggacc gcagaaaact tgttgagtta aaaaacgaat ttctgaccat tctttcggat
    32941 ctttcgttaa acattgcttc ttacaccggg gatgatttta atcctaactc tcccaaacag
    33001 ctttccgaga tcctttttga aaagctcaaa cttccaaaag gaaaaaaaac caagaccggg
    33061 tacagcactg atcacaatat tctaagcagt cttgcggaac ttgacattca cccgctgcct
    33121 gcactgataa tcaaaaaccg ggaatattcc aaactcgttt cgacatatct tgacccgatg
    33181 ttttcaatgc tcggtgaaga tgaccgcctg cgaacgacat tcattgtaac acacgccgca
    33241 acaggtcggc ttgcgagcag ggaaccaaat cttcagaaca ttccggtgag aactgatgaa
    33301 ggaaaaaaga tccgtgaact tttcatagcc gaaaaaggaa aaaaacttat ttcccttgat
    33361 tacagtcaga tagagctgcg gatactggca tctttaagcg gtgaagacga atttatcgaa
    33421 gcttttaaca aaggagagga tatccacaaa agaacagcgg cggcaatatt tcagaccata
    33481 cctgaaatgg tcgatgcaaa catgagaaga cacgccaagg cagtcaactt cgggatcatt
    33541 tacggaatgc aggcgtataa actctcgata gacacaggtg ttgacatagg tttcgcaaaa
    33601 aaatatatcg acaattattt tgcatgttac ccgaaaataa agaatttcat cgactctacg
    33661 atcagaaaag ccgtagaaac gggattttct gaaactctgc ttaaaagaag acgatatatt
    33721 cctgaactct ccagtccgaa caggaacatt gcaaaatcag gagaaagaat ggccgtaaat
    33781 acagtcattc agggctcggc ggcagatata attaaaacag ggacgatcca tgttcacaga
    33841 atgctctcag aaaaatatcc ggatgcgagg atccttcttc agatacacga tgagttgata
    33901 attgaagccg gcggagacta tgaaaacaca gcccgtgact gtgccgctga gctttccaag
    33961 tcgggtgaac ttatcgatgt taaactggat gtgaattatt caaccggaaa caactggtcg
    34021 gagcttaaat gaaaaaattt tccattattt tgcaaaaact tgaaataatc acccttcctg
    34081 atatattttc tatgctttta tgatattgat aaccttgaat tggaggattt tatgcgtcac
    34141 atttttttaa tgatcgcgat tcttttttca ttttctcttt ttgctcagga accgtctctt
    34201 gttccaatag ctcctgcaga agaaaagaaa gaagaaaaga aagaagaaaa aaaagaagag
    34261 aaaaaagaag aaaagaagag tgattaccag gatttatacc agcaatatct gaacaaatac
    34321 aattctgatc agaaacctgc tcagacaact gaaccggctc cggttcagac agctccggct
    34381 ccagctcagg aacctcagcc ggcagctccg gtaactgaac agccgcagga acagacggct
    34441 ccggctccaa atctcgtacc ggtagtcgag gcagctcctg ttgtagaaga agtaaaacct
    34501 ccaaaggaaa ttgttccgct taaaccattt gaagtgaaac cgtattttgc cctttcaaca
    34561 ttcggtgcaa tgaataatgc catttatttt ttcggcaagg attcgctctc atcaagctac
    34621 aaattcgctc tgaacaatgc cattcttgat cttaaaggcg gcaacaaaat gttcgattca
    34681 aggattcttc ttgattttgc caaaggtctt gatacatcaa aagttattgc aatcaattat
    34741 gagcatgatc cttctttgga aacagatgct tcaaaccctc ttgaacttga tgttacacct
    34801 aatcagccga acacactgga tattatcagg gacgtttctt ttaaaatgac ccacccgaca
    34861 tatagaaaag acggattcgg aataaatctc ggtttccagg caggaaaatt caacatgcct
    34921 ttcggtcttg aaacacttta tgatcatgag cttactttca gcagtaactc atatatgaac
    34981 tccgtttttc tgggagaggg tttcaacgat ctcggattaa ctctcggtgc ggactttctc
    35041 ctttcaaatg aaatgaacct cggcttcaaa ttcttccttt tcaacggttc aaacgaaact
    35101 atgcttgacg gacaggacaa atttcaggat cccgctatcg gattcgatct tagattcaag
    35161 tttgacagca aattttatac gacagtagca ttctcattca tctggggaag cgtttaccat
    35221 gactatgatg aaaccgtgaa agacggtatt tttgacacaa ccaacggtac ttatttgaac
    35281 caggaagaag agatcgaagc tgacaggatc gaaaaaacag caacatcgaa cgattacgca
    35341 ttgaacaaga agaacatcct tctttcaata ggcgcagatc tcggatataa gcttaacgac
    35401 aacatttcga tcggactcat gacccagttc gcattttcaa gcagatcgat ttttaacccg
    35461 cagagaattg atgagaacgg aaattcacac attgatgcaa atcagaaact taatgatgac
    35521 ggcaatggcg accaccttag atttctcaga gacggaagca ctaatttctg gggaacaagc
    35581 aattatacga catggggtat ttttgccgct ccatatacaa aactctacat gtttgacata
    35641 atggcccgca tcagttacaa caaagcacct tacctctata aatttcttga ggacagcaac
    35701 aactcttcat tggctttcga tcttgatatt gtttacaatt tctgtgatta cggttcactt
    35761 gcacttacct atactttcgt aagggaagag ataaataaat acgacagaga ttacaacgat
    35821 aaaaataatt tagacgaata cacctacatc aaagatattt tcaatcataa catcatcgct
    35881 ttgaatcttt ttgtaaatta tgatttcctt tgggaaaaga actgattttt aaagatcttc
    35941 ctcagtcatt tttctataga tagttctctg tgaaacatca agaagttccg ctgcttttat
    36001 tttgttaccg tcagtatact taagagcggc cgatatcact tccttttcga tctccttcat
    36061 cgatttttca ccaattctga aacttaagac cggaggaaca tgctgtgcct tcttgtccgg
    36121 atgtccgatc tctgattcac tatttccctg aaccattccg cctgaagtaa gaacatatct
    36181 cgctatgaaa ttctcaagtt ctctgacatt tcccctccat tcctgtttta aaagttctct
    36241 gacagtttcg ttatcaggat aatggggctg ttttttatac tgcctgcata atttatcaat
    36301 aaaatgcttt atcaactcag gtatatcctc cttcctttct ctgagaggcg gaatggtaag
    36361 aggaaaaaca gaaagacggt agtaaagatc ttctctgaac tttccttttt ccacttcttt
    36421 ttcaagatct ttattagttg cggcaataat ccggacatct gaaaataccg gaaccgcact
    36481 gcctacagga agatattcct ttgactgaag aaatcttaaa agttttttct gtaacgaaac
    36541 gggcatctcg ccgatctcat ctatgaaaag cgttcctcct tcagcagcac cgacatagcc
    36601 gggactgttc ctttcagcac ctgtgaaagc acctttctta tatccaaaaa gctctgattc
    36661 cattagagat tcactgacag ccgcggcatt gaaagccaca taaggtctgc cggcccttga
    36721 actttctttc aaaacatgtt tggccagaag ctcttttcct gttccgcttt cccctgttat
    36781 gagaacagtt tcttctgtct ttgctacctc cgatgcattt ttgagaagct tttccattga
    36841 atcgcttaca tagacaattt tatatctgtc ggtgttgata gaccggatct cttctttaag
    36901 ccgcttgttt tcctttatga taacagatgt ttctattgct tttcccgcct gttgcagaag
    36961 aactttgaaa tttaccggtt tggtgagaaa attatatgcc cctttctgca ttgccttaac
    37021 cgcagtttca acatttccgt aagccgttat cattattacc ggaatcactg tctcaagatc
    37081 tttgaccgca tcaaggatgt ctattccgtc aaagccgtca ggcaaagcca gatcagtaat
    37141 tataagagac acttcttttt caagttcctt aattatttta agtgcttctc tggcatttga
    37201 agctgttaga actgtatatc ccgatctcga aagtatcaac ttcatggagt caagcagaga
    37261 ctcttcatca tcaattacaa gtatcttcct gttcatggcg gcctcccgga cttccaaaac
    37321 aatcaaactt cgggaattgt agcttttttg tccaattact gcaagtgatt tcttgaaaac
    37381 accttccttt ggaatataat gtgcctgtat gcaattttgg aggtagcaat gaaatatttt
    37441 tttatggcag cgttaatcat tttctgctta tcagggtgcg gtgagacaaa accggaaaat
    37501 gttgaattga caataactct ttcgttccct gaaggaacaa catgtaaaag ctatctggcc
    37561 gatcatttta ctgtaacgct gtataactct gaacagaaaa agactgactc acaagacttc
    37621 ccctgtacgc cggaaacaga tgcacttgtc ctgtatgttg aaaaagacat ctattacttt
    37681 actgttgcat tgaaaggcac agacgaactt aataaaagtt atggatcagg cactgtcgac
    37741 ctttcaaacg gggatactga aattaaaatt gaaatggaag aatatcaggg cggtattact
    37801 tttaaatgga acggatctga ctgtgagaat ttcggcatcc ataacttgaa gtttacgctc
    37861 gaaaatgatg acggttatgt ttcttccgta atatggggca aggaagaaaa aatcgaagat
    37921 tttgaaatcc tctgcagcgc agaaatgctt gagatagtaa acatccctgc tgttttttac
    37981 actgcatcgg caaaaggttt cagaaccgaa gaatcaaaaa caccgagaat catttattca
    38041 gtttctgata aatttaaagt tgtttcagga caggatcaga aaattgatat aaatgaatat
    38101 aaagaagtta tggtgtctga tataaaaata atatgggaat ttgacagcaa aagtattgca
    38161 agctgtgaag atgccggagt tgccagtata attgcctctc tcgtttcaaa cagcgacaca
    38221 ctttctgaag agacaacctg tgataacagc ttcagccctt tttactttta tgatgtttct
    38281 gctggaaaat acgagcttct tttccgcgga cttgacagtt ctgagaacac tctgtttgaa
    38341 aacgatgtga acacacagga agttacagtt gataaaggtt tgatcggatc agatataata
    38401 aacataaata ttctgctcaa agaaaaataa ccttaaagaa ggagaacgat atgcttacta
    38461 aaaaacagct cgataaaatt aagaaactcc ttaatgaaga gcttgccgag attgaagaaa
    38521 gaaaccgtga aaaagtaaat gaaatggaaa atgagggtga tgcggaaacc tttcacggtg
    38581 acgaagccga tcaggcgaac tatcttgaac acagaaacag actcctccgt ctgcgcgaca
    38641 gagacagaaa gctgataaac aagatccgtc agacacttga aaagattgaa gaaggcgagt
    38701 acggaacgtg tgaatcatgc ggctgcgaca tcggatttga aagattaaaa ctcagacctg
    38761 tagcgtcttt gtgcatagaa tgcaaacagg aagaagaaga aaaagaagaa agagataaaa
    38821 ctctcaaaag aagatagctt tgacaagtgc attaagaaaa tcctttcatt ttaacggagt
    38881 ataaattgaa atctctgata attgtaacac ttttactctt ttcgttcctc acgcctgatg
    38941 tgctgactaa aatggttgat gctgtataca ttcctgaccc tgtcatcgtt gttgaaggtg
    39001 ctgatgataa aggaacccct gttattctgg aatacgaaaa aaagactgga acgctcataa
    39061 aacattcggg ggacagggaa aaaggagaaa agagcaccga ttttgacctt ttccttaaga
    39121 ttttcttctt tcccggagaa cacacttctc ccgatactat gaaaaaagca tctgagacta
    39181 ttgccggact tttgaaagca aaagggatca aaactgagaa ttgcattgta agtgccattg
    39241 atgacggggg tgatcccgtt atcagtatcg gtaaagaaaa aagattctca aattcaaacg
    39301 aacttgcagt tttcaaagga tcgtatcttc catcaggtct gacagtaaat gaagaaacaa
    39361 ttgtcttttc agaatatcat aaaagtgttc tccccgctgc atttccaggc aggatcgaat
    39421 tttacagcaa cggaaatctt ataaaaacat ggatgttcta tcgaaaagaa caccggcaat
    39481 gatcctgtgt gcagaatatg actgaaaaat ttgcatcagt tctaataccc agacctgaac
    39541 tgtcacaact cagttatact attcctaaaa atcttgatat taaaacagga gactgtgtag
    39601 aaattgaatt gagaaaaaca aatatatggg gaattgtatc agggatctct gattcacctc
    39661 cacccgaaat ggatggaaaa ccgctgaaac ctgttcttgg aaaaatcctt tcctcccccg
    39721 tttttgcaga cagaattgaa acatctcttc taaaatggat agcggaatac tatctctatc
    39781 cttttcctaa aattatcaag cagatattct cgccattgat cggaccgggc aacactctat
    39841 acggctcatc ggatattcag aaacacttct catccctttg cacatgcttt tctcaagaaa
    39901 aaagagagct taacattcat caaaaaaggg ttgtagattc gatccttaag aagtggggag
    39961 acagagacta caggccgact ctggtttttg gcatcacagg aagcggaaaa agtgagattt
    40021 ttgcagaact atgcaaagat acaataaaga aaggcaaaca ggtcctatat cttgttcctg
    40081 agatcgggct cacgtctaaa gcattagaac accttttaaa caggattggt tctcccggag
    40141 ttatcataca cagcttcatg tcaaaaaaga aacggttttc ctcaatatat tgtgcaatgt
    40201 caggtgaagc gaagatcatt gtcggaacca gatcttcagt tctctatcct ttttttgata
    40261 tcggtcttat tgtaattgat gaagaacatg actcaagcta taagaatctt gagcccccct
    40321 actacaatgc aagagatttt gcagtaatga agggggcgaa actcaaaatt cccgtagtaa
    40381 tgggaagcgc aacaccttct tcagattcat ggtacaactc attaaccggg aaatatcaca
    40441 ttgaaataat taaagaaagg gctaacaaca aacctctgcc tgaaattaag atgttccctt
    40501 atatcgggga tctttatctg cctggagaca taatttcgaa agtcagagaa tcaattcttt
    40561 caggtgatca atctttgttt tttctcaaca gaagagggtt tgcaacactc gcactgtgtt
    40621 ccgagtgcag aaaaacagta aaatgtcccg gatgcaacac cgctctcata tatcataaga
    40681 aaaagaaatc tctcctgtgc caccactgca acttcagcag aaatgagttc aattgttctg
    40741 aatgcggatc gaccttaaaa tttgaaggca tgggcataga aaagctggtt gaagcagttt
    40801 ctgaatattt tccgggagcg gaaatagtat cctttgacag ggataatcta acaacactgt
    40861 ctctttttga caaagcggtg gaaactattt caaaaaacaa aggaaatatt attgtcggaa
    40921 cagtaatgat atcaaaaggg cataacttcc cgaaacttaa aaatgtaatc ataaagtatg
    40981 ctgactatat tctgagtttt caggattcaa gggctgctga aaaatgtttt caactgatag
    41041 cgcaagtcgc aggaagagca ggcagatttg atacggcagg aattgtttat gcagaagcgg
    41101 tttatcctga tcactatctt tggaaatatt tgaagaacaa tgattatgaa ggattcataa
    41161 acgaagaact gagttggaga aaaaagcttt ctcttccacc atacacaaaa atgatgatca
    41221 tccgtatttc cgggaaaaat gagaaaaaag taaatgatac agcagaatcg cttcactttt
    41281 acatcaatag atctcttgaa gaaaccggaa acggtcattt tattcttttt cctgtatcgg
    41341 aacctcctct ttcaagaata aaaaaccgct tcaaaaagaa cataacttta atagcaccga
    41401 aaaataccgc cgatttgaaa aaattaaata agataattgt cggtcttccc gactttaaag
    41461 gtgtttcagt gacctttgat gttgatcctt tgaacgaaac atgatgtttg attacttaaa
    41521 aacaattaga tcggtctctt tggatttttc acaatattca acatcatcaa accgggaagt
    41581 aattgtaagt gtcggtaaaa gcggctgttt tatgtataat tccttgattg ggcaacatcc
    41641 tgaggtcaga aatctacatc gattgataat agttccttca gccgcagaag ccgatgtgga
    41701 actattaccg gatatcattt tttcatcgca tccgaatata actgagcaaa gtttttatgc
    41761 atgcggcaaa ttatttgaat ttattaaaga aaatcgcccg gagaaaatca cagtgctttt
    41821 aagcggcggc agttctgcct taattgagaa aagtgctgat cctgaaaaaa cagtaagaac
    41881 aaacgagctt cttttgaaaa gcggactgaa catacttgaa atgaaccgga taagaagcga
    41941 aaactcactt ataaagaacg gtaaattcgc ggcacttttt cccaaaatta tctggcagat
    42001 tttcgttatg agcgatatcc ctttcaaaaa tggcgagaaa ctcgtcggaa gcatgccttt
    42061 tttcagagaa gatctgaaaa acacagcttt attcaagtgt gctgattcca acaccctcca
    42121 cgatcaaatc agctcatttt ttgaaaatgt taattctata aaaaacttta caggaacagt
    42181 taatgaattg gcggacatca ttaaagagtt cataaataaa aaaacaggca atcttgttat
    42241 cagcggagag ccaactctta aaattgatca cccctctcct ggcagcggag gaagaatgtc
    42301 ccatctcgct ttaaaacttc ttcccctcat tgaaaaaagc atgaagctat atgccctcag
    42361 ctctgacgga atcgatggta actctggttt tgcaggagcc gtcattgaaa atgtatccgg
    42421 aaattcgttt aatatcactg aaattgaaaa ttcacttgcg aactatgact cagcagcttt
    42481 tcttaataaa tataatatga ccctgaaaag tggttatacc ggcattaatt tgaatgattt
    42541 tgtgatatta ttaagatctt agtagcagta tccctgaata caaggcattc ctgaacaaca
    42601 ctcactaaca cctg
//
LOCUS       DAOOYB010000004        38600 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_888_length_38600_cov_9.340174, whole genome shotgun sequence.
ACCESSION   DAOOYB010000004 DAOOYB010000000
VERSION     DAOOYB010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 38600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38600
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_888_length_38600_cov_9.340174"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..1031
                     /locus_tag="PL056_00555"
     CDS             15..1031
                     /locus_tag="PL056_00555"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HPM45534.1"
                     /translation="MRFYLLMIAILVVSGCATASPESNYAKTYASAYAKGFEDGFKKA
                     SDLKMPEKPQASALPQYDQYGYPYYDNYGGYSGAPQKGTLSYSVQTGDMKSVKEIIEG
                     KKAGVNDLDQSGYSPLQIAARMGFIDIMEYLIKNGANINFASSDGYTPLKEAITYKKF
                     SAADILIAKGADTNYIDSAGYSMLYYSCASGNKKLVETILAKGASPNVVASNGYGPLI
                     ASVSMGFEDIAVLLLEKGANPDETDNYSGFTALMYAVRNNKPKLVELLAPKSKNINFS
                     EKTGKTALHFAVEYNRLDMVKVLVNSGASVDVKDSSGNTPVDTANQRNLKDIIDFFKS
                     TKKI"
     gene            complement(1028..2371)
                     /locus_tag="PL056_00560"
     CDS             complement(1028..2371)
                     /locus_tag="PL056_00560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPM45535.1"
                     /translation="MSVNTPVDLLKGEPSKTLLAISIPAIILNILKAGYNIVDMFWLG
                     QLGKEYLAGVSASVFLVWAMHGLSALVTVGIVAGISRNIGEGKLETARSNTWRSLKLA
                     FALGLLLTAFLFPLIDPLVGMIKLEPVAHKAGTEYLQIMISSTVAGFLMFALHSVMIA
                     WGDTRTPVKVYAATFIFNIFFSPVLMLGLGPFPRLETRGAAIATVASYLLGSVIFFGV
                     ILKNRWILFQKAGHEIPFKRYISVGYPVALSGMFFSFIYFFIAKITAMFGSDAVGAMG
                     IGHKIESLAYFFSHGIAAGLSTFTGRNLGAGMEQRARDAAGAGMRFVSIVTGIYSIFT
                     FIFAPQIVALFNSDPGLVVQGTNYVRIVMPVELLQSILMTIEFGAFSGSGYTKPSFFV
                     SLPIVFLRIPLAWILAVHFGLNANGIWLTIATTMALNSFIFMILFKKDNWLKARV"
     gene            complement(2362..4134)
                     /locus_tag="PL056_00565"
     CDS             complement(2362..4134)
                     /locus_tag="PL056_00565"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HPM45536.1"
                     /translation="MNLISINDITSIKGDNVLFERISLGISENEKTALVGVNGCGKST
                     LLKIMAKKEEPESGNVSVNRNIKISFLEQMPVFDPAETVREHIFNRKKDVPSESDKRI
                     EEILGKLGISDLSLKMAELSGGMIKKIAIAQVLVEDSDILFLDEPTNHLDIDSIVWLE
                     EYLKKSKKSIVMVTHDRYFLDSVCSSIYEIERSTIYHYKGSYDFYLKKKEEHLNSMIS
                     NEERIKSILRRELEWLARGPRARGTKSKERIESIRKMVDRDRPEEEKSIEFSVSGRRM
                     GKKILEMGDICYKWGDAPLIEGFSMVFKKDTRLGLIGDNGSGKTTLLDIISGRMAPQK
                     GIYDIGVNTKIGYFDQQARELPLTMRVIDYVKRKGERINLPDGSSITASQMLERFLFD
                     PALHYLDIGKLSGGERRRLYLVSVLMENPNFIILDEPTNDLDIKTLSVLEDFLNSFPG
                     PVVVVSHDRYFLDRTVDSLIILKGDGTFEGFTGRTSDWLEQKKKAPLKTDSGNMAQKE
                     ETAKPRHISDTKPKMTYAQRIRFAELEKLIPDIEREIAEVELKLANYSNTGISSGDLF
                     LQYETLKKKGDNLMNEYIELSECQ"
     gene            complement(4142..5302)
                     /locus_tag="PL056_00570"
     CDS             complement(4142..5302)
                     /locus_tag="PL056_00570"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPM45537.1"
                     /translation="MKKIFIVFLFIFFAGCASTPVKDEPKDTKEEQLRVVESTHALYV
                     TPKSVELMKLEDVDKKERVFEEDLNNIFEMANKFFDLRRYEEAEKHYRNLLEKNPDHP
                     SSAYCYYNLGLIYMRVTRWDDAETSFLQAYNAFEREQDKKDALLNYFESLKKAEKWAS
                     LLYESEKFINNNPFKLNFHEEAKQEISLRYAESLIMTGKVEEGRKLAEYWIFEITKKL
                     PRYEAIYIPELSLAYYVFGRSFVYEFNMLKLDTTTDKLEEKCKKIIEAQTQFIKSINV
                     GVIFWTNASAYEVAKLYMDLYAEMEGHPVPQELNDEEKKCYKCELWKKISNLLKKSRK
                     TLVASIDAAKKINEENEYTQMSFKMIEEIDRIYESKEADCKVFEEVKSVKAQ"
     gene            complement(5320..6858)
                     /locus_tag="PL056_00575"
     CDS             complement(5320..6858)
                     /locus_tag="PL056_00575"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HPM45538.1"
                     /translation="MKKIRQLLIFSFFLCSILAAQNSEFDFMHSASFKFSKGVPFIRA
                     HIKGYHSGVEVKNVTSFKCNGKTVKTGSFRVFVNKFNSQEVMYKLSFQELSVPELKSY
                     AGTLAKWEEDAEIPMEIYVHGAIFSINTLKIDNREYYIAARELLSRESAYKQLAKYRS
                     EFPGSNISLIPVLTKPSSSVIKVKSQDGKNHECLNIVTVGKESFFYTGDDRYGSNSEF
                     FFTASSDKSLELSIEDSVENLVKQILPGEMFLSAPLETLKAQAVAARTDIFMQLGKRH
                     VSEPWHICSEVHCQKILWGKEIDPKFVAAVKETEGMVIMFKGSHIARAPYCSSSGGKT
                     EDIRHVWFSAEKQYLKGVWDGETPLELDLSKEDDLKKFLDSDYGEDNIPMNKKHRWSV
                     TYRQEELDEIVNKSLNIGTIHSIEALARGVSGRIYKAVFKGSGGTETVYGELNIRKIL
                     NNMNSSAFIVSKGEGIWTFSGAGWGHGVGMSQMGAISLGRKNKDFKYIMKRYYPGTEV
                     TKIY"
     gene            complement(6827..9175)
                     /locus_tag="PL056_00580"
     CDS             complement(6827..9175)
                     /locus_tag="PL056_00580"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HPM45539.1"
                     /translation="MRVSVFFLFFISFTTVYGYHECLNQDEVSEECIKEIYSEKEYTF
                     VIGQIDDVKFNNKNDGLILKIDSLFRSANYDEIEKNINKLPMNLRNTLYYKVLLLKTS
                     FARKRFNQTLEMIESVKKEYPYYYVSSDLECVKADIMLFRKQYDGALENYDRCLKIRS
                     NIISSYNRIVALEKNGSPKITFLKDYLEYYESNEGTFLTSRIVDRLVQMKKNSNLPNA
                     ASPYYSRWLAVMRKSSKLDLFFNDEYLTISLPANSEIIKYLVGKKRFSDALSMIDASI
                     AKLEKDVEMKFNYVVEKYRTLVAAGQDKAAADYMVSVADQFSNSKKDRLLFFAGTIYF
                     DAGFVFESKKILEEMVYKNQASKYFLLALFKLGLIYLSEGSDLYTFSLWTNYIYGNGL
                     NYSKFVSGQKVYEAISGLIGTIDRLNGFCTFSSEIDPDCQLGSVCQQNNGSRFITYYD
                     FLYFHLTGMVDFSKKIDVSDKSYVNKWKENTFSAEVDEEIILERFDMLSHAAKKIEPA
                     VMIKQFVLLNNRDGVEFYLNYVNSVIGFNERKNYSPDIKFLEKKAYEEQSVRFSEFRY
                     KTDVVFKHYFRKTGDLADHYYNLVSPELTYSPHYGKIEEWKLIYPTPFIDEILKLSVE
                     FEVPVQLIYSIMRAETYYRDDLSSNVGALGLMQIMPVTFEKISKFGGIKINDPMNPYD
                     SMKASVWYLSKLLKRFDNNYAAAVAAYNAGPHRVSEWITRYKGSEKYIFVEMIPFHET
                     RNYVKKVLRFMEIYSYLYEGVFYDMGLKGVINIEENPAVVNF"
     gene            complement(9279..10511)
                     /locus_tag="PL056_00585"
     CDS             complement(9279..10511)
                     /locus_tag="PL056_00585"
                     /inference="COORDINATES: protein motif:HMM:NF023428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HPM45540.1"
                     /translation="MTTPYVPLYSALRPQTFDDIAGQESVIKLIRSFLSKDFLPSMIF
                     YGPPGTGKTTIARIASEQFGAKMYRFSAVVDGVSEIKKKIYADSGSVLVGRQIVFIDE
                     VHRFNKAQQDIFLPMIESEGVIFIGATTENPSFYINDALLSRARCIKFSKISSSETLS
                     VLKKAASHLNATIPEDLQSVIAQESGGDLRIAISILESAFHLSSNGVIKKEDVMELIE
                     NPQKYDKKGDYHYRTISAFIKSLRGSDPDAALYYLVKMVESGDDPLFLLRRMIIFASE
                     DVGNADPRALQLAVSALEGFKAVGFPEGKIILSHIVTYLATAPKSNASYMALKEAEDF
                     YRNNPDLKIPQYLINSSSLAPDEEKGEYRYPHDHKDQWVDQRYFPGEGEPASFYKMKN
                     SGYESKVKAYWERIKKGV"
     gene            complement(10508..12106)
                     /locus_tag="PL056_00590"
     CDS             complement(10508..12106)
                     /locus_tag="PL056_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45541.1"
                     /translation="MLKHLVLLVIAGGTLLFFMIKWSGEGDYDRFQKAREYSEYYKEF
                     FVESPLNYKVFDDMPLVIRATNLGSDFWNKEFYGMIRQSENEVNEILSVAKLINETHE
                     DGGNIVKYEQNLSSFPALFELFTDGADLSGRTLFNLACGEIFEFWERETRNYKRKTSA
                     YAKAETVAGYLFDNPSLAPVEIQNRKRLLENLTKEKAEASVSKDEIIKKIKHYADQSF
                     ANFLDNALNRPSSLTDAEQLALLISVLKHIKMHSETAVLPKMENLKSFIPYAKVENIQ
                     IDEVKKSVRITNPNTSVPVSFFKEALFMKKLREKLPKENFHYLIFVGDRHGMWRLPEK
                     FMRWSVSIDDPYAVTNKARGAIKLMAEEGFFVVTRKNEELILLKMDDLSARIKKDIAE
                     MTDSESRNSYMAGLRKEHPSRKYLFDPVKINVFQRFDTPVDWRNGSSVEKNLSALVID
                     SDPVKARMLSYSLFVSDMDDVKKFETEFPSTVFSVLRYDDTVYVPDKLKENFMTLEDI
                     EKAKLQFEYRSKGLNPDGSVPEKK"
     gene            complement(12166..12408)
                     /locus_tag="PL056_00595"
     CDS             complement(12166..12408)
                     /locus_tag="PL056_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005995515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HPM45542.1"
                     /translation="MMKEFIEFIAKKLVDNPDAVDVSFIEGKNTAIIELRVAPEDVGK
                     VIGKSGRTANALRTLLSAVASRQSQKIVLEIIEEKK"
     gene            complement(12543..15044)
                     /gene="gyrA"
                     /locus_tag="PL056_00600"
     CDS             complement(12543..15044)
                     /gene="gyrA"
                     /locus_tag="PL056_00600"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014257138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HPM45543.1"
                     /translation="MTEIIHGKIKDIAIEQSMKTAFIDYAMSVIMDRSLPDVRDGLKP
                     VHRRVLFSMKELGNTWNSSYKKSARIVGDTIGKYHPHGDQAVYGTLVRMAQTFSMRYV
                     LVDGQGNFGSIDGDSAAAMRYTEARMAKIAGEILADIDKDTVNMRDNYDGSLKEPEVM
                     PTRIPTLLINGTSGIAVGMASNIPPHNIVEIINAVIHLIDDPEATVVDLMQHVSGPDF
                     PTGATILGRTGIRTAYETGRGIIKIRSKARIEQADNSNSRDRIVIDEIPYQVNKSNMI
                     IQIAELVKEKKIEGIHDIRDESDRRGIRVVIELKKDADGNILLNQLYKMTQMQVSFGI
                     NMLAICNGVPKTINLRDTINHFILHRKDVVTRRTRYELNEAEKRAHILEGLKRAIDNI
                     DEVIEIIRGSASSDDARNSLMSRFAFSRPQAVSILEMKLSRLTGLERDKIVSEYEELL
                     KKISWYKEILTNDSTLMGVIKAELKEIKEKYGDERKTDIVNYEGDLDMEDLIADEDMV
                     VTLTTENYIKRTPLDLYRKQKRGGKGINAINPKENDYVKDIFVASSHTLLLIFTSYGK
                     VYWTKVYQLPEAGRNSRGKPIINLVNVEKDERVAAILPIKQFVEGCFIMMGTKKGTVK
                     KTDMMEYAIQRTNGKKAIRLNEGDELIEVKITKGDDDVVLATKAGLSIRFNEKDVRPM
                     GRVAAGVRGIRLAKDNEVVTMDVIEPDTTLLSITDKGIGKRTPIDDYRLQSRGGKGII
                     TIKTSAENGNVVGVLKVNETDEAMLITSNGQAIRIPVSGISVIGRNTKGVRLFRVAEG
                     EHVVSITKIVDPEDSSIEAEDAVDQEAPEEDSASE"
     gene            complement(15059..17509)
                     /gene="gyrB"
                     /locus_tag="PL056_00605"
     CDS             complement(15059..17509)
                     /gene="gyrB"
                     /locus_tag="PL056_00605"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007634719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HPM45544.1"
                     /translation="MTDVNEKLALELQEEKEYGAASIQVLKGLEAVRKRPGMYIGDTD
                     DGNGLHHMIYEVVDNSVDEALAGHCDKISVYIHVDESITVKDNGRGIPVDIHKEEKRS
                     AAEVIMTVLHAGGKFDNNAYKVAGGLHGVGVSCVNALSEWLTLKIKRQGGEYYQEYKR
                     GVPVAPLKRIGETSEKGTKITFKPDPQIFTMTEFSLPRVVKRLREMAFLNSGVKIYLL
                     DERVGQEFEFHYDGGIKEFVSYLNESRPGLHPEIITVSRHKDQGNLTMDVALQWTTSY
                     QEQTLCYTNNIFNRDGGTHLSGFKLGITKVFQNYVAENRKNAKIAINGDDIREGLTAV
                     LSVKMADPKFSSQTKDKLVSSEIQTFVSNAISESLTTYLEENPDIAGIIIDKIMEAAV
                     AREAARKAREMTRRKGALENTTLPGKLADCQEKDPAKSELYLVEGDSAGGSAKQGRDR
                     SNQAILPLRGKILNVEKARLDKLLKSESILTIIAAMGTGIGKDEFNIAKLRYHKIIIM
                     TDADVDGAHIMTLILTLFYRYMPEMIEKGYVYVAQPPLYGITKNKKITYVKDEKEFQQ
                     YLVDSGAQSLSLTIGDKVLSGKDFKKFVEMLVELNDNLTKTEFIYDSSVVEAIAATTR
                     FNVDDFKSETALWEKWETIRTYLETNHPIKAPFRMELEENTRYAGEFKMKIHTTVNGV
                     DKVTMVDYKYVNNSEIKLINSLKEKILEAGCCDYVLKNTTRDEELTVSGWKNLLNEIL
                     VIAREGQKIQRYKGLGEMNPEQLWETTMNPENRVLLRIRAEDAAEANRIFDVLMGENV
                     EPRRRFIEENALNATIDI"
     gene            complement(17699..19054)
                     /locus_tag="PL056_00610"
     CDS             complement(17699..19054)
                     /locus_tag="PL056_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45545.1"
                     /translation="MKKIDLLFLLTFIFTAPLFAFDAEERCEPSIIFPEIFFLESPGC
                     FSFDTANTVYLNGTDPLKKYLDMFDNAKDINIYRYLYYLRRNENFPALMVLMLMTSNG
                     SIQGSEGLFYNEFNNLIVGGIKESENSGEKKKLHLSNDSEPVSVKDLSINFFSALSYI
                     AAYVMEDNRSKNIDGEILVRTFHYIISNIQGGIDWDDDTYINDLFNSFIIMKNQGLLQ
                     SFTEIFYFYLRSDRNIPPFNDYVKLREIGMVKDMRPFPVNGSVAQSEKKVFASFFLEN
                     INQVDRIVFIANPGDLVYSDNSETCEYLNQRGEWLEKLKDADPADTIFNESVKIVEQC
                     PDSFHLPFKIEKGEVKVYSGAKWIADLAFAVSYIIKEDLSDDESKIIIEGAMRSSLGK
                     NREISDKKLAEDVLSYLWETGKAESVIKALFARKISLEKGDENADSITEAAEIIADIL
                     F"
     gene            complement(19051..22008)
                     /locus_tag="PL056_00615"
     CDS             complement(19051..22008)
                     /locus_tag="PL056_00615"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPM45546.1"
                     /translation="MKFQISNSVKMERTVQLSELVLSCEFLEGSSLYHERSAIILSVE
                     KSESGFKISGKIRNREHTAVLFSIDSTGRIVSATCSNGCKGVCRHIVAMGLLFNELSM
                     ENADISEQLSVLGRKNIQTRTDFPLKITINKKDVKVLYDAGDYQLKKTEEIFGGKNLI
                     FLVSLFPEKFMLGNTLLSPSVSPLTLFYDVDNEAVRFFFSDHIHYFDHEGVAVNSETG
                     NVWRLPASVNETVSTLLNISMGYSRKDDLVKAIADLADNLTPFVSLNGELNLQKIELD
                     DKTNVLFDTYIENGKVCVSIFFIHSGEKIEFMPKRKNYDQNYPLNGRVYSISPDLIKK
                     IRNALSSEGFRQGRNCYQAPMSAFSGIIKKDSALNSAGMVVSRKEIKRISFDEETADK
                     TEIVLDINSKEKWFSFNIKLPESANLISSAELVSAVMQYEKGIAEPVVADMNGMPVIL
                     EKSSGFLKKIAEFSFSELFRSKDKISTVHLLKLLKTKNRKILNSFTGDDDSQQKYLQI
                     INSLSKGELPDVSTEHLSGIPIRNYQIEGYKWMMLLRELGLGGILADEMGLGKTLQSL
                     AMIKSENDDKPSLVVCPKTLIWSWDREIEKFFPEMKRVVIDSLKPEERVSRWKKAEHE
                     LIITSYAVVINDFKYLKDKLFKTVVLDEAQHIKNNNTKRFKTLSSLKTEHRFALTGTP
                     LENHLSDLWSIFQFIMPGYLGSRKDVDRSEKNNENEGLERIRSMASPFILRRTKKETV
                     SELPELIIKEYPVEMTPKQKEIYLSVLLRGRAEFIEKGDSINKIEVLSILSNLRLAAD
                     HPALVSKTDTDPALSSKIPAILELTDEIIQGGGRVLIFSQYVKMLKIIASAFDFQGRD
                     YFYMDGTTQNRIEMVESFNKGEKEVFLLSLKVGGVGLNLTGADNVIIVDPWWNPATEE
                     QAWSRAHRIGQEKKVIVNKLFSKGTVEERILDMHQKKRGMTDFFLSKSLKDPSIDFVK
                     MIADMEFSAMEKL"
     gene            complement(21989..22642)
                     /locus_tag="PL056_00620"
     CDS             complement(21989..22642)
                     /locus_tag="PL056_00620"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer-membrane lipoprotein carrier protein LolA"
                     /protein_id="HPM45547.1"
                     /translation="MKFLQTVLLILFVSTVYAQDAAVEKLLGKYNGIKSFSSDYNRVF
                     TQNSTGKKSTDNGTILFIAPAEIRMDTFFEGKLTEQTFLNSEKTVFIYHSKKNILVKK
                     SAGDEGEYLAFLQGLDEVRKKFIISDSTNSIEKAKKTGIEIKEGSMLYKLTPKKDMGN
                     VKYIFITSVKDEIESVVIIDQLKNINQFKFSNIKYNPKLEKKQFAPVYPQGYEVSNF"
     gene            complement(22656..24608)
                     /locus_tag="PL056_00625"
     CDS             complement(22656..24608)
                     /locus_tag="PL056_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45548.1"
                     /translation="MTARIFKLIPAVVFLVLLSACDSSSEDYFFEQPSSFSVLKGSKC
                     LNGTVSYMGFVVDNTQKYGARLMKVSGCSSKIDKDFKDELDDKTGIYINGTGVSSDQI
                     IVDGEVIFATASSGYTEFEHWKNKDDELRLEKHAGRIVFRKMGVDMKHDASFNRSILL
                     DCYPSIVKAYKGEGFFFSGSRFGRNYLGFSDIAGKGDYEEVGFTVSDIAVAGDRAYIL
                     DSLSGSVYETDSSLKYAVKLTDPELVGAGMTSIGQNRIVFYNKTDIRVVNSSLDTIKS
                     LKMFDGNEISSVTSVKYDQTFEYREFSGERMNEFVEIYKKEEVDDSDTLIEDVETDDD
                     ETPDEDSDNSGETILDASEGDVLWIASKQGNVMAYDMKNSSWIVVKYSESEELSNPDY
                     YNEMRPYIDSTYTTYPNNGRTEPSNAPFISSVYAVRGLPHSITYKFIYEGIIEDTFSR
                     NGKLDEGGTVLSDETADFEKFSIDPLTDSITLLNRINSVDCVIPWNENAVFPITDVIS
                     SSQLAVKIDKYKDQIEKCYGSPFSYAIFPKERYSVSRESYYGPEFAGKGVELSAGSNE
                     SVLSFSDEYAGISIKRKTDDVVTERETAFFIKLSPGVPFAGFSSPDAFTFMANPASGK
                     VLLFSPLSRRVIEYDIPVSKILKIYK"
     gene            complement(24608..25789)
                     /locus_tag="PL056_00630"
     CDS             complement(24608..25789)
                     /locus_tag="PL056_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45549.1"
                     /translation="MSTAPFRIIATAFLLITILGCSDETKLIYPPEDVVNRVTDFYLF
                     KDGTTFMYLNSNMNRKYDFGSIVIMEIDDEGEMTYIDSIIVPSLAGKMAVNSEESMVY
                     VTSRDRHGIVRVKISGKAGAYRLSYIDDTDGDIPEILETQKEPYAIILNEDEDKLFVT
                     HLLNGEFSIVDLEKWELLETHKLKYGVTDIAYDAVSGYYLASHRSSGNITLIEPSESL
                     SGFNVGITETEIDLPTDGYDIRSLKASSDGVSFYASFQNYSDDSDEDSAPQLLKFKLA
                     GKKSTETELVETIALKGSLGEISVLPYVTGSDENEYNGELIFITSPGQKILYIVDSGR
                     DEILDQIKFDKCEPYQVSASSTGNTTGIVLVSCFAQDTVYLYGIDIDNKDLYEEIGGI
                     K"
     gene            complement(25786..26709)
                     /locus_tag="PL056_00635"
     CDS             complement(25786..26709)
                     /locus_tag="PL056_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45550.1"
                     /translation="MRLFQVLAVILLISLMNSCIALKKDVDMASSQLTAENNAKIKVL
                     EESLRSEIAKMSLRLDEIEKTLQIDKKAQENKINLSFNTLDELKSTIRDINNRIDSVD
                     IAAQQGGGLSSKVSELEKAVKLYEEQLEKLKSDMLMQISELKPVENLTVTENGVVRLP
                     ENEEKSYNQLVDFTRSASDGSVARKGWDVYSLKWPKNRRCDVAYWTGESYFLEKSYNN
                     AIEYFGKIESEFNTCSKLEASYIRTAYALFYIGKEDVASKVLEAMQIKFSKSAFAEQI
                     KDLKKMISGNAAKKKTPAGKNEKDAKKGDKK"
     gene            26973..28103
                     /gene="hisC"
                     /locus_tag="PL056_00640"
     CDS             26973..28103
                     /gene="hisC"
                     /locus_tag="PL056_00640"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HPM45551.1"
                     /translation="MKENLSCLNTGRPLVSSLIKDVVAYNPITSRDKILSKQTYCDLP
                     PLKLDWNESTLPPSTTVKNALVEALACEENLLNWYPELFSKDLRQKIGEYCNRQMEEV
                     LVTNGSDDALDLICKVFLEPGDEVVIPYPTYTHFVTYVQSRGAVLKKVEAPDPFKPDI
                     ASIIENLSPATKLIYVVNPNNPTGVLISKKQISTLCSVARNAIVIVDEAYFEFAGETV
                     MDLIDRHSNLIITRTFSKAWALAGLRVGYLVTNIILIDELSKVINPKSVSVLAQIAAA
                     AALSDKKYMDDYVIEVKNSKMMLLQFFRSRNIVAHDSSANYIMVQHPKLPRFIEEMEK
                     ENVFVRDRSSFKNLPGFFRITLGDHIQTDDLITRLDKALSRV"
     gene            28199..28747
                     /locus_tag="PL056_00645"
     CDS             28199..28747
                     /locus_tag="PL056_00645"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPM45552.1"
                     /translation="MEKLLKDNLETIFSYSLFVTGNRDKALDLMQDAIVTALSKKHLY
                     KEESHFRSWIFRILKNTHLNNIKRAQIKKESYLADISKENEETPSFLMASLTTLEEMS
                     DPLLNQKMSQVLEIMPAEYKDAIILKEIEGLSYEETAKQLQIPVGTVMSRLHRARGYL
                     KKAFRKEAVELKIVTERVKKNG"
     gene            28740..29552
                     /locus_tag="PL056_00650"
     CDS             28740..29552
                     /locus_tag="PL056_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HPM45553.1"
                     /translation="MVETKCDMNELFIEQYADNELDLRESSEVSIHLGSCVSCKKRYE
                     EIIFAKNAVNRFNSLNKLSLIEKEGFYSFIDQHSSKYGIVHKFFEYLRSHGFTVAVST
                     VSFTSLVFAFVFSLSQIEKENNLIIKEIIAAHNNLLPYDFDTPEKTETELNKKFTIDK
                     KTLLNLASISPVLRGRFTSIAATPAAKISIKGLKEEEKGTLFLSKNNAHIKNVFEDGT
                     CLVKHPDNNCKAKLIQKQGNDMIHWEELDNDFVFVTDNNRMSAQMVQLISNY"
     gene            complement(29553..32687)
                     /locus_tag="PL056_00655"
     CDS             complement(29553..32687)
                     /locus_tag="PL056_00655"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HPM45554.1"
                     /translation="MKRFISVFIFISTITVSGLSKDVSLNQFMIDKWDNSSGLPQNSV
                     TCITQTRDGYLWMGTEEGFVKFDGLKFKLYDDSVLPIETHNTGFIIEDNKTPHLWVGF
                     NGGGLVKLNYENDKFTVYDTKNGLPENAVKYGVQTLNGMLYLGTARSGVVLIKPDESI
                     SLIDKSAGLPNNNVKFIGKTKDDTVWVATYNYLSKIEGSTAKEVLKLSYEINTVYFES
                     PKNFIIGTMGGGIYSFNPMTGDVFPYKKNQFGDKMVSAIYLDSHKCVYVGTLNDGFFR
                     ICNEEDSKSNWLPINHAVAIISDHEGSLWIGTRGSALHRLKEGKFITYGRKNGLSEPV
                     IFPITESSDGGMWIGTWGGALYKLKDNKIKQYTFGDHLAKYDTILSLFQEKNGPLWAS
                     VYGKGILKIDDNETRLYSDSDGVIEKLIPAIFKDSRGVLWVGTQTKGLLYMEENPPFK
                     LFPGTDGMNISSIAEDRSGNIWIGTHNGAFRIQGETLVKDFTNLENIATLSVYPASDG
                     KIIFATDNGLTVYEKSMGAITIDRKKGIDTRKIFDVIEDRTGGLWLTSNKGIKYIEKR
                     DLEDFLQGKVPVVTPKTYGFKDGLLTPECNGGTQPNIWRSSDGKIWIPTAEGAAVVDP
                     SDTIINGVVPPVHIVSVKGDDVIYQVMPDEKIIFEAGTATVSFEFTALSYLFPDQVSF
                     KYMLDGFDDKWKEAEGKRYTFYTNLEPGDYTFKVIAANNDNVWNTEGTSLKFRIEPFF
                     WQTLWFKTLLVIAFLTFLVIWIKRKVVQIRSREDVLSKTIQSRTKNLKDIILHVKTLS
                     ETLADISKLISSNTGITAEKFNATYAMIDTASSTLSDITSKLSDTRNEVQDMSQVLTK
                     ISKKADHSTIVLGEAVDSIEKIESSANQVSNIVEVVDEIAFKTNLLSLNAAIEAARAG
                     ESGKGFAVVAESVRELSMQTANAVEMIKKLIDDTNLKVVSGRSSVNNIVSFINELVGE
                     FRSISNQISQIKGIIENHVNEVGSVDRSLSDIRRITQESTEMVDNVYQVSRKLNVETA
                     NLRKEVTKIQDIDN"
     gene            complement(32749..33633)
                     /locus_tag="PL056_00660"
     CDS             complement(32749..33633)
                     /locus_tag="PL056_00660"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPM45555.1"
                     /translation="MLYFILTLIIFASMEAVSKPLMGYIDPFALTFWRFAAGFLFFLF
                     YPGMKKRFKEIMHFNKKDWLAVISLGILNTFLAMSILQISVKHCSAATSATIFCSNPV
                     FVMLISSVLGLEKFSLRKSIGIFTGVGAVAFIMAEKGFIINSGALFALSAAIVFAAYT
                     VLSKSTVSKIDPFAVNLTSFFSGLTANFIFMSAADISFVPSAEFFNVSRTLAFLYLGF
                     VVTGAGYVTFFETIKRFTAVSSSIIFMLKPALAVLFSAIFLNETLSAHFFAGLLMLTA
                     ATAVIFSDRFPDIIRKYK"
     gene            33757..33843
                     /locus_tag="PL056_00665"
     tRNA            33757..33843
                     /locus_tag="PL056_00665"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33791..33793,aa:Leu,seq:caa)
     gene            33954..35213
                     /locus_tag="PL056_00670"
     CDS             33954..35213
                     /locus_tag="PL056_00670"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HPM45556.1"
                     /translation="MFQDIRKKSSSILVSLMFAAIIVTFVISFGPGDDAGCSSSAQNY
                     AASVNGELITTSEFRFSYSNMYDYYQRIFRDFNNEMAAQYKLSEKAMDGLIGEILLAQ
                     EAKSLGFKVSDEEVRKEIMETPYFQQNGAFDRDSYNKMVQFTLNTTVSDYEHKVRLQL
                     LSRKLRSFLFQSIDISETEVLDEFTSSDEKMDAFVYSILESSLKDEIKNKIKDEISDD
                     EVKTFLEADLTKVKLTFEDNVSKYSKKDGSGQTLFEDVKVDVAKDIMLKDKIAAKMKL
                     DSEAVFTAVKENPSITTEDISKKFPDWNISRKEVPGVVKNAKFVQGVGFSPKFVEKLF
                     GSTEPGVLDSVFITEEGNHVIAGRIKHVPADMEKFEKDKGNIKERLKTTKKAQILENY
                     VDQLRDKATVSINENFLKIYSSPAQAE"
     gene            35221..37062
                     /locus_tag="PL056_00675"
     CDS             35221..37062
                     /locus_tag="PL056_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45557.1"
                     /translation="MTSEKFITILSTAIVLITIVFSSVVFVKELVKPSDVLERTLLDN
                     CRENKFPEDKINDENELENIASDCVFISDELAESSEFTKKFKNLQHLKKFDYLFLGKK
                     GILLKRNTVDHKKDAEYIYKCFKDAACSDQGSDESITITYLMQDSKGEVAISTTYNDL
                     PAHQTVKNFKKLHKEISAIKNLSADLRLTLLFHKSYTFIEDKTPENVISLLKRKVDGL
                     FLKSRGAKIRVLPWEYSDNPVNVLDRKSRQYGLDKEEFKKDEASVYHYRTVQFIEKNG
                     EMVPWLGIYNDKKSNYSDNLAIRMISKHLKNIQSSDGSFPAEMDIFEGADKKSKDNLN
                     IQAETAIALFKASAVLKDGALADSAVKAVGYLIKKNLSAEVSSALLLYILSLCGDQCS
                     LDEHAEKKDELVKFFSDMANIRKSIADNPVYSGIFLNAFSASSEKLKDQEIELLKNAL
                     DKYSGLNHSDKLRFISYLMWTDYKKDSETFEMVRSFVNRETVFIKESLFDDRGFNDFS
                     GSFMSSAKKSIPDTAISVLIASGLSRAVSKDYHNDFIFETESLSGNFLRFLIVTDDDF
                     PNWGSSKAKSSVQGGVRVFPGAGTVKLANSTRVLEYFVNRAIKGRAR"
     gene            37059..37577
                     /locus_tag="PL056_00680"
     CDS             37059..37577
                     /locus_tag="PL056_00680"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HPM45558.1"
                     /translation="MNGFICKICGACCKGEGSVFLYPDDVKRIAENLSMTLQETINKY
                     TEYVMMETIEKSGSYSYLPFLILKKNKGACVFSNSNLCEIHKFKPFQCMHTPFVSEFF
                     SDESWRKQLMAECPALGKMKKEDFAVYAATGEMSEKAEQDYYLLLKENSFNLEKILSV
                     KLGTPEILTYHD"
     gene            37570..>38600
                     /locus_tag="PL056_00685"
     CDS             37570..>38600
                     /locus_tag="PL056_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45559.1"
                     /translation="MTDLTTTKQNTIQEWDIIMNWIGEKAISEYGKRSLFRQDALSRA
                     ESVKEFEMIVEASTLSETSKGVFSFNLDSVTGVVERFEKNEPLEAKDYRIIGDFLGSI
                     RKSGEEIKKEKWASGINLILPADFDISFEMTIKQCITDKGEVSSNATPELAKIRKQLS
                     DTHKHIGIEIKKMILSPEYSSYLQEDWFTVRDDRYVLPFKSVFKRTMKGVIHNYSRTG
                     QTAFLEPLPLIEHNNALSLLAAKELEEIIKVLKELRGLVYRKYEYLKKCIETALHLEN
                     IYTKHKWMKEYGCVIPEFTDRELRIESGWYPPVFLTAGKSLVKNDFIFNDEEKIMVVS
                     GPNAGGKTVA"
BASE COUNT        11060 a         8371 c         7175 g        11994 t
ORIGIN      
        1 taacggaggg gaaaatgaga ttttatcttt taatgatcgc gattctagtt gtttccggtt
       61 gtgcgacagc ttcacctgaa tcaaattatg caaagaccta tgcatcggct tatgcaaaag
      121 ggtttgagga cggttttaaa aaagcttccg acttaaaaat gcctgaaaaa ccgcaggcct
      181 ccgccctgcc tcaatatgat cagtacggat atccttatta cgacaattac ggcggataca
      241 gcggcgcacc gcagaaagga acactctcct actcggttca gaccggtgac atgaaatctg
      301 taaaagagat cattgaaggg aaaaaagctg gtgtgaacga cctcgatcag agcggataca
      361 gccctcttca gatagccgca aggatgggat tcattgacat aatggaatat ctgataaaga
      421 acggtgccaa catcaatttt gcttcaagcg acggatacac tccgcttaag gaagcaataa
      481 cttataagaa attctctgcc gcagacatcc tcattgcaaa aggggctgac acaaattata
      541 tcgacagtgc cggatattca atgctctatt attcctgtgc gtcaggcaat aaaaaacttg
      601 ttgaaacgat ccttgcaaaa ggggcttcac ctaatgtagt tgcatcaaac ggttacggcc
      661 cgctgatcgc ttctgtcagc atgggttttg aagatattgc tgttctcctt cttgaaaaag
      721 gggcaaaccc cgatgaaacc gacaactatt ccggttttac agcattgatg tatgccgtaa
      781 ggaacaataa gccgaaactt gtcgaacttc tcgccccgaa atcaaagaac atcaattttt
      841 cagaaaaaac aggtaagact gcacttcatt tcgcagttga atataaccgc cttgacatgg
      901 ttaaagtgct cgttaattca ggagcaagcg ttgatgtgaa agacagctca ggcaacaccc
      961 cagttgacac agcaaatcag agaaatctca aagatatcat agactttttc aaatcgacaa
     1021 agaaaatcta aacgcgtgcc ttcaaccagt tgtctttttt aaaaagtatc atgaatataa
     1081 agctgttaag tgccatcgtt gtcgcgatcg taagccatat tccgttagca ttgaggccga
     1141 aatgcacagc cagaatccat gcgagaggaa tacggaggaa aacgataggc agggaaacaa
     1201 aaaatgaagg tttggtatag ccggaaccgg aaaatgcacc gaattcaatt gtcatcagaa
     1261 tgctttgaag aagttccaca ggcattacta tccgcacata gttcgtcccc tgaaccacaa
     1321 gtccgggatc actgttaaaa agtgcaacta tctgaggagc gaaaatgaaa gtgaatatcg
     1381 aataaattcc ggtaactatg gagacgaatc tcattcctgc cccggcagcg tcacgagctc
     1441 tctgttccat tccggcgccg agatttcttc cggtaaatgt tgaaagccct gcggcaatac
     1501 cgtgagaaaa aaagtatgcc agagattcga tcttgtgccc gatccccatt gcgcctacag
     1561 cgtcagagcc gaacatggcg gtaatttttg caatgaagaa atatatgaac gaaaagaaca
     1621 ttccggaaag agcgacagga taaccgacag agatatatct tttaaacggg atctcatgac
     1681 ctgctttttg aaaaaggatc catctgtttt ttaatataac gccgaaaaat ataacagatc
     1741 cgagaagata gcttgcaaca gtggcaattg ccgcacccct tgtttcaagc cggggaaaag
     1801 gtccgagtcc gagcatgagg accggactga aaaatatgtt gaaaatgaat gtggcggcat
     1861 aaactttaac aggtgttctc gtatctcccc atgctatcat cacagaatga agtgcgaaca
     1921 tcaggaatcc ggcaacagta gatgagatca ttatctgaag gtattccgtt ccggctttat
     1981 gtgcgaccgg ctcaagcttt atcatgccga caagcggatc aataagggga aacaaaaaag
     2041 cagtcagcaa aaggccgaga gcaaacgcga gtttcaatga tctccatgtg ttgcttcttg
     2101 cagtttcgag cttcccttca ccgatgttcc ttgaaattcc tgcaactatt ccgactgtga
     2161 caagtgccga aagcccgtgc atcgcccaga caaggaagac cgaagcactg acaccggcaa
     2221 gatattcctt tccgagctgt ccaagccaga acatatccac aatgttgtaa cccgctttta
     2281 aaatattaag tattatcgca gggatggaaa ttgcaagaag ggtttttgac ggttcacctt
     2341 tcaacaggtc gacaggtgtg ttcactgaca ttcagaaagc tctatatatt cattcatcag
     2401 attatccccc tttttcttca gcgtttcata ctgaaggaaa agatcgccgc ttgatatacc
     2461 ggtgtttgaa taatttgcca gttttaattc cacttcagct atttcccgtt caatatcagg
     2521 tatcagtttt tcaagctcgg cgaacctgat gcgctgtgca taagtcattt tcggctttgt
     2581 atcgctgata tgtctgggct tggcagtttc ctctttttga gccatgtttc ctgaatctgt
     2641 tttaagaggc gctttctttt tctgttcaag ccagtcggaa gttctcccgg taaatccctc
     2701 aaaagtgccg tcacctttaa gaatgatgag cgaatcaaca gttctatcaa gaaaatatct
     2761 atcgtgactt acaactacaa cgggacccgg aaaagagttc agaaaatctt caagaactga
     2821 aagcgttttt atatcgagat cgttagtcgg ctcgtcaaga attatgaaat tggggttttc
     2881 catcagaaca gagaccagat aaagccgcct tctctctcct cctgaaagtt tcccgatatc
     2941 aagataatgc agtgcaggat cgaacagaaa tctttcaagc atctgggaag ctgttataga
     3001 tgaaccgtca ggcaggttta tccgttcccc cttccttttg acataatcta taactctcat
     3061 tgtaagaggc agttccctcg cctgttgatc aaaatagccg atcttcgtgt tgacacctat
     3121 atcgtaaatc cctttctgtg gtgccatcct gcctgaaata atatcaagaa gcgtggtttt
     3181 cccggaaccg ttatcaccga tcaatccaag ccgtgtatct tttttgaaaa ccatcgagaa
     3241 accttcaatg agcggtgcat ctccccattt gtaacatata tcgcccattt caaggatttt
     3301 cttacccatc cttctgcccg aaacggaaaa ctcaatgctc ttttcctctt ccggtctatc
     3361 tctgtcaacc atctttctta ttgattcgat ccgttccttt gatttcgtcc ctcttgctct
     3421 cggtccccgc gcaagccact ccagctccct tctcagaatt gactttatcc gctcctcatt
     3481 tgatatcatc gagttcagat gctcctcttt ctttttgaga tagaaatcat aagatccctt
     3541 gtaatgataa atagttgaac gctctatctc atatattgaa gaacataccg aatcaaggaa
     3601 atatctgtcg tgtgtcacca ttacgatgct tttcttagat tttttgaggt attcctccag
     3661 ccagacgatt gagtcaatgt caagatgatt tgtcggctca tcaagaaaaa gaatgtctga
     3721 atcttcaaca agtacctgag ctattgcgat ctttttgatc attccgcctg acagctctgc
     3781 catttttaaa gaaagatctg aaatgccgag cttcccgagg atctcctcta ttcttttatc
     3841 tgattctgag ggaacatcct ttttcctgtt gaaaatgtgc tctctgactg tttcagcagg
     3901 atcgaacacc ggcatctgtt caagaaaaga gatcttaata tttctgttca cggaaacatt
     3961 gccggattcg ggctcctctt ttttcgccat gatcttcaga agcgtcgatt tgccacaacc
     4021 gttgaccccg acaagggctg tcttttcatt ttcagaaatg cccagagata ttctttcaaa
     4081 gagcacatta tcccctttaa ttgaagtaat gtcgttaatt gaaataagat tcatttgtga
     4141 tttactgtgc ttttactgat tttacttctt caaagacctt acaatcagct tcctttgact
     4201 catatattct gtcgatctct tcgatcattt taaagctcat ctgagtgtat tcattctcct
     4261 catttatctt ttttgccgca tcaatgctcg ccacaagggt ctttctcgat ttttttaaca
     4321 aattcgatat cttcttccac agttcacatt tataacattt tttctcttcg tcgttcaatt
     4381 cctgtggaac cggatgtcct tccatttcag cataaaggtc catataaagt ttggcaactt
     4441 cataggcact tgcatttgtc cagaaaatca cgccgacatt tatcgacttt atgaactgag
     4501 tctgtgcctc aatgatcttt ttacatttct cttcaagttt gtccgttgtc gtatcaagtt
     4561 tcaacatatt gaattcataa acaaaactcc ttccaaaaac ataatatgcc agagaaagtt
     4621 ccggaatata tatagcttcg tatctgggaa gtttcttagt tatctcgaat atccagtatt
     4681 cagcaagttt gcgaccttct tcaaccttgc ctgtcatgat cagcgattca gcatatctga
     4741 gcgagatctc ctgtttcgcc tcttcgtgga aattgagctt gaacggattg ttgtttatga
     4801 atttttcact ttcataaaga agtgatgccc atttttcagc ttttttaagt gattcaaaat
     4861 agttgagcag tgcatctttt ttatcctgct cccgctcgaa tgcgttgtat gcctggagaa
     4921 aactggtctc agcatcatcc cagcgggtca ctctcatata aataagaccg agattgtagt
     4981 aacagtaagc cgatgaaggg tgatccgggt ttttctcaag aaggtttcta tagtgttttt
     5041 ccgcttcttc gtatcttctc aagtcaaaga atttattagc catttcaaag atattattca
     5101 gatcttcttc aaagacgcgc tcctttttat cgacatcttc aagtttcata agctcgacac
     5161 ttttgggagt cacatagagt gcatgggtcg actcaacaac cctcaactgt tcctcttttg
     5221 tatcttttgg ctcgtcttta accggtgttg aagcacaccc tgcaaaaaag ataaaaagaa
     5281 agacaataaa gatctttttc atggtttccc tccgggtatc taataaattt tagtaacttc
     5341 agttccaggg tagtatcttt tcattatgta tttgaaatcc ttattctttc tgccaagaga
     5401 aattgccccc atctggctca ttcccactcc atgtccccat cctgcaccgg aaaatgtcca
     5461 tatgccttcc ccctttgaaa cgatgaaagc ggaactgttc atattgttca ggatctttct
     5521 tatgttgagt tctccataca cagtttctgt tcctccggaa cccttgaaaa cggctttata
     5581 aatccttccc gaaactcctc tggcgagtgc ttcaatggaa tgtattgtcc ctatatttaa
     5641 cgatttattt acaatttcgt caagttcttc ctgtctgtat gttacactcc atctgtgctt
     5701 tttattcatc ggaatgttgt cttctccata atcagaatca agaaattttt ttaagtcatc
     5761 ttcttttgaa agatccagtt ccagtggcgt ttcgccatcc catactcctt taagatactg
     5821 tttttctgca gaaaaccaga catgccggat atcttctgtc tttcctcctg atgaagaaca
     5881 gtaaggggct cttgcaatat gcgacccctt aaacataata accattcctt cagtttcctt
     5941 gacggcagca acaaacttgg gatctatctc ttttccccag aggatcttct gacagtgaac
     6001 ttcactgcaa atgtgccagg gttcagaaac atgccttttc ccaagctgca tgaagatatc
     6061 agtccttgcc gcaactgcct gtgctttcaa tgtctcaaga ggagccgaaa gaaacatttc
     6121 acccggaagg atctgcttta caagattttc aacagaatcc tcgatcgaca gctccagcga
     6181 cttatctgaa gaagcggtaa agaaaaattc ggaatttgat ccgtatctgt catctcccgt
     6241 ataaaaaaaa gattcttttc cgactgtcac tatgttgaga cattcatggt tctttccatc
     6301 ctgagatttt actttaatta ctgatgatga aggtttcgtc agaaccggta tgagcgatat
     6361 gttgcttccc ggaaattcac tccggtattt cgcaagctgt ttatatgcgc tttctctgct
     6421 tagcagctcc ctggccgcaa tataatattc cctgttatct atctttaaag tgttgatgga
     6481 aaagatcgca ccgtgaacat atatctccat cggaatttct gcatcttcct cccatttggc
     6541 gagagttccc gcatacgact tgagctcggg cactgaaagt tcctgaaaag agagtttgta
     6601 catgacttcc tgagaattga acttattgac aaagaccctg aaggaacctg tcttgaccgt
     6661 tttgccgtta cacttgaaag aagtgacatt ttttacttcg acacccgaat ggtacccttt
     6721 tatgtgagct ctgatgaaag gaacgccttt gctgaattta aatgatgcgc tgtgcataaa
     6781 atcaaattca ctgttctgtg cggcaagaat tgaacacaga aaaaaactaa aaattaacaa
     6841 ctgccggatt ttcttcaata tttatcactc cctttaatcc catatcataa aagacgcctt
     6901 cataaagata actgtagatc tccatgaatc gcagaacttt ttttacatag ttccttgttt
     6961 catgaaaagg gatcatctcc acaaaaatat atttttcgct tcccttatat cttgttatcc
     7021 attcagaaac cctgtgagga cctgcattgt atgcggcaac agcagcggca tagttgttgt
     7081 caaaccgctt cagaagcttt gaaagatacc acaccgatgc cttcatgctg tcatatggat
     7141 tcatggggtc atttatcttt atgcctccga acttacttat tttttcaaat gtcacaggca
     7201 ttatctgcat taatccgagc gccccgacat ttgaagaaag atcatccctg taatatgttt
     7261 cggccctcat tattgaataa atgagctgca caggcacttc aaattcgaca gaaagcttta
     7321 atatctcatc aatgaaaggg gtcggataga tcagcttcca ctcttctatc ttgccgtaat
     7381 gtggagaata tgtgagttca gggctcacaa ggttgtagta gtgatcagca agatctcccg
     7441 tctttctgaa ataatgttta aaaaccacat cagttttata tctgaattct gaaaatctga
     7501 ctgattgctc ctcataagct tttttctcaa gaaactttat atcagggctg taatttttcc
     7561 gttcattaaa accgatcacc gagttcacat aattcagata aaactcaacg ccgtccctat
     7621 tatttaaaag gacaaactgc tttatcatta cagccggctc tatctttttt gccgcatgcg
     7681 aaagcatgtc aaaccgctca agtattatct cttcatcaac ttcggctgaa aaagtgtttt
     7741 ctttccactt attcacataa cttttgtcag aaacatcgat ctttttgctg aaatcgacca
     7801 ttcctgtcag atgaaaatat aagaaatcgt aatatgttat aaatctgctt ccgttgttct
     7861 gctgacacac gcttccgagc tgacagtccg gatctatctc tgatgaaaac gtacagaaac
     7921 cgttgagccg gtcaatcgta ccgatcagtc ctgatattgc ctcatatact ttctgcccgc
     7981 tgacaaattt tgaatagttc agtccattcc cgtagatgta atttgtccac agcgagaaag
     8041 tgtaaagatc gctgccctca ctgagataga tcaatcccag tttgaaaagc gcaagcagaa
     8101 aatatttaga agcttgattc ttataaacca tctcctcaag tatttttttg ctttcaaaaa
     8161 caaatccggc atcaaaatat atcgttccgg caaagaaaag aagccggtct tttttggaat
     8221 tggaaaactg gtcggcaact gaaaccatat agtcggccgc ggccttatcc tgtcctgctg
     8281 caacaagagt tctgtatttc tcgacgacat agttgaattt catttcaaca tctttttcaa
     8341 gcttggcaat tgaggcatct atcatgctta aagcatccga aaaacgcttt ttgcccacaa
     8401 gatactttat tatctcgctg ttagccggga gagagatcgt cagatattca tcattgaaga
     8461 aaaggtcaag cttggagctt tttctcatga cagcaagcca tcttgaataa tacggggacg
     8521 cggcattcgg aagattgctg ttctttttca tttgaactaa gcggtcaacg atcctgcttg
     8581 tcaggaatgt tccttcattc gattcatagt attcaagata atccttcaga aatgtaattt
     8641 ttggagaacc gttcttttcc agcgccacga tccggttgta tgacgaaatt atgtttgatc
     8701 ttattttcag gcatctgtca taattttcaa gtgccccgtc atactgtttt ctaaagagca
     8761 taatatccgc cttgacacat tcaagatcgc ttgaaacata gtaataagga tattcctttt
     8821 tgacagactc tatcatttca agtgtctgat tgaacctttt tctggcaaaa ctcgttttaa
     8881 gcagaaggac cttatagtaa agtgtgtttc tcagattcat cgggagctta ttaatgttct
     8941 tctcgatctc atcataattt gctgatctga aaagagagtc tattttcaat atcaagccgt
     9001 cattcttatt attaaacttt acatcatcta tctgtccaat gacaaaagta tattcctttt
     9061 cgctgtaaat ttccttgatg cactcttcag aaacttcatc ctgattaagg cattcatgat
     9121 acccgtaaac agttgtaaaa gagataaaaa atagaaaaaa aacactgact ctcattatcc
     9181 gttaaaacct cgcgaatgcc tactttttca gggtattata aattctattt aaggtcaact
     9241 attttttgta attcccggga acacaaaaaa gacggtggtc aaaccccttt ttttattctt
     9301 tcccaatagg ccttcacctt ggattcataa cccgaatttt tcattttata gaacgatgcc
     9361 ggctcgcctt ctccgggaaa atatctctgg tcgacccact gatctttgtg atcatgcgga
     9421 tacctgtatt cccctttttc ctcatctgga gccagtgatg atgaatttat gagatattgc
     9481 ggtattttaa gatctggatt attcctgtaa aaatcctctg cctctttaag tgccatatag
     9541 cttgcgttgc tttttggtgc cgttgcaaga tatgtgacaa tatgactgag aataatcttt
     9601 ccttccggga atccgactgc tttgaaaccc tcaagggccg atacggcaag ctgaagtgct
     9661 ctcggatctg catttcccac atcttcggaa gcaaaaatta tcatccttct caacagaaaa
     9721 agaggatcat ccccgctttc gaccattttt accagataat ataaagccgc atcgggatca
     9781 ctaccccgca aacttttaat aaatgcagat attgttctgt aatgataatc acctttttta
     9841 tcatatttct gaggattttc aataagttcc atcacatctt ctttttttat cacaccattt
     9901 gaagaaagat gaaaagcact ctcaaggatc gaaattgcta tcctcagatc tccacccgat
     9961 tcctgtgcaa tgaccgattg cagatcttcc ggaattgttg cattaagatg gcttgccgcc
    10021 tttttcagaa cactcaaagt ttcactggaa gatatttttg aaaatttaat gcatctagcc
    10081 cttgaaagca gagcatcatt gatataaaac gagggatttt cagtagttgc gcctatgaaa
    10141 atgacacctt cgctttctat catgggaaga aaaatatcct gctgtgcctt attgaacctg
    10201 tggacttcat caataaaaac tatctgcctt cctacaagaa cagagccgct gtctgcatag
    10261 atcttctttt ttatttcact cactccgtca acaactgccg aaaaacggta catcttcgca
    10321 ccgaactgtt ctgaagctat ccttgcaata gttgtctttc ctgtgccggg aggtccgtaa
    10381 aatatcattg aaggaagaaa atccttagac aggaacgacc ggataagctt gattacactt
    10441 tcctgtcctg ctatatcatc aaaagtttgg ggtctgagtg ctgaataaag gggaacataa
    10501 ggtgttgtca tttcttttca ggcacggaac catcgggatt aagacctttt gagcgatatt
    10561 caaactgcag cttggctttt tcgatatctt caagagtcat aaaattttcc tttagtttat
    10621 caggcacata cactgtatca tcgtatctga gcacagaaaa caccgttgac gggaattccg
    10681 tctcaaactt tttcacatca tccatatctg aaacaaacag tgaatacgac agcatcctgg
    10741 ctttaacagg gtcggaatct atcaccagtg cagaaagatt cttttcaaca gaggatccgt
    10801 ttctccagtc aacgggagta tcaaatcttt gaaaaacatt tatttttacc ggatcgaaca
    10861 gatactttct tgaagggtgc tctttcctta aaccggccat gtagctgttt ctgctttcgc
    10921 tgtctgtcat ttcagcaata tctttcttta ttctggcgct cagatcatcc atttttaaaa
    10981 gtatcagttc ctcgttcttt cttgtaacca caaaaaatcc ttcttcggcc ataagtttaa
    11041 ttgcacctct ggccttgtta gttacagcat acgggtcgtc tattgaaact gaccaccgca
    11101 tgaatttttc aggaagccgc cacattccgt gtctgtcacc gacaaatatc agatagtgaa
    11161 aattttcttt cggaagcttc tccctaagtt ttttcataaa aagcgcttct ttgaaaaaag
    11221 agaccggcac acttgtgttg ggatttgtta tcctgacact ttttttgact tcatctatct
    11281 gaatattctc aacttttgca taagggatga aacttttaag gttttccatc ttcggcagaa
    11341 ctgccgtttc cgaatgcatt ttaatgtgtt tgagcacact tatcagcaga gccagttgtt
    11401 ccgcatctgt cagagatgac ggacggttta aagcattgtc cagaaagttt gcaaaacttt
    11461 gatctgcata atgcttgatc ttttttatta tttcatcttt tgaaacgctg gcttcagctt
    11521 tttcttttgt aagattctcc aaaagtcttt ttctattttg gatctctaca ggggcgagcg
    11581 aaggattatc aaaaagatat cctgcgaccg tttcggcttt tgcatatgcc gaagtctttc
    11641 ttttatagtt tcttgtctct ctttcccaga attcaaatat ttcaccgcat gcaaggttga
    11701 aaagagttct tcctgacaaa tcagctccat ctgtgaaaag ttcgaacagt gcaggaaaag
    11761 aagaaaggtt ctgctcgtat ttcactatgt ttccgccatc ctcatgtgtt tcatttatga
    11821 gttttgcaac actcagtatt tcattcactt cattttcaga ctgtctaatc attccgtaaa
    11881 attctttgtt ccagaaatct gatccgagat tggttgctct tatcacaaga ggcatgtcat
    11941 caaaaacttt gtaattcaaa ggggattcta cgaagaattc cttgtaatat tcagaatact
    12001 cccttgcttt ctggaagcgg tcataatctc cctctcccga ccatttgatc atgaaaaaaa
    12061 gaagcgtccc tccggctatt accagaagca cgagatgctt cagcataaaa gtatcctttt
    12121 tcctttcggc gaactccgag gcccgcactt ttcttcgagg aaacattact ttttctcttc
    12181 gatgatttcc agaacgatct tctggctctg acggcttgca acagcactaa gcagtgtgcg
    12241 aagcgcattt gcagtccggc cgctctttcc gatcacttta ccaacatctt ccggagcaac
    12301 tctgagttcg atgatagctg tgttcttgcc ttcgatgaaa ctcacatcta cagcgtcagg
    12361 gttgtcaaca agcttttttg caatgaattc aataaattcc ttcatgatcc cctccttttt
    12421 gggatggaaa tacaataaac aaaatgtcta atagcataga actaaggatt ttattattgc
    12481 acaacctgaa acaaattgca acttaattat tttacaaaga ataaatccgg ataatggaaa
    12541 cattattctg aagcgctatc ttcttcaggc gcttcttgat cgactgcatc ttcagcttca
    12601 attgaagagt cttcagggtc aactattttt gttattgata cgacatgttc gccttctgca
    12661 actctgaaca gacggacacc tttcgtgttt cttccgatta cacttattcc gcttacaggt
    12721 atcctgattg cctgtccgtt ggaagtgatc agcatggcct catcagtttc attgaccttt
    12781 aatacaccga caacattgcc gttttcggca gaagttttta ttgttattat tccttttccg
    12841 ccacggctct gaagacggta atcatctatc ggagttcttt ttccgatacc tttgtcagta
    12901 atactcagca gtgttgtatc cggttcaata acatccattg tgacaacttc attgtccttt
    12961 gcgagtctaa tacctcttac acctgccgca actcttccca taggtcttac atctttttca
    13021 ttgaatctta tgctgagccc cgcctttgtt gcaagaacaa catcatcatc acctttagtg
    13081 atcttgacct ctatcaattc atcgccttca ttcagtctta tcgccttctt gccatttgtt
    13141 ctctgaatcg catattccat catgtcggtc tttttaacgg ttcctttctt cgttcccatc
    13201 ataataaaac aaccttcaac aaactgtttt atgggaagta ttgccgcaac tctttcatcc
    13261 ttttcaacat taacaaggtt gattatcggc ttccctctgc tgtttcttcc tgcttccgga
    13321 agctgataga cctttgtcca gtaaaccttt ccatatgagg taaaaataag gagaagtgtg
    13381 tggctgcttg caacaaaaat gtctttaaca taatcgtttt ccttgggatt tatggcgtta
    13441 ataccctttc ctcccctctt ctgttttctg taaagatcaa gcggtgttct cttgatatag
    13501 ttttccgtag tcagcgtcac gaccatatct tcgtcagcaa taagatcttc catatcaaga
    13561 tcgccttcat aattgacaat atcggtcttg cgttcgtcac cgtacttttc ctttatctct
    13621 ttcagttctg ctttaattac acccataaga gtggagtcat tcgtcaggat ctctttatac
    13681 caggagatct ttttcagcag ttcttcatat tcggaaacga tcttatctct ttcaagaccg
    13741 gtaagacggc ttaacttcat ttcaagaatg gagactgcct gaggtctgct gaatgcaaaa
    13801 cggctcataa gactgttcct tgcatcatct gatgaagctg atcctctaat tatttcaatg
    13861 acttcgtcaa tattatcaat ggctcttttc agtccttcga gaatatgagc tcttttttcc
    13921 gcttcattaa gttcataacg ggttcttctt gtaacgacat cttttctatg aagtataaag
    13981 tggtttattg tatctctgag attgatagtt ttcgggacac cgttgcatat tgcgagcatg
    14041 tttatgccga atgaaacctg catctgagtc attttataaa gctgattgag gagaatattc
    14101 ccgtcagcgt ctttcttaag ctcgataaca accctgatcc ctcttctgtc ggactcgtct
    14161 cttatgtcat gaattccttc tattttcttt tctttcacaa gctctgcgat ctggattatc
    14221 atattggatt tattaacctg atacggaatt tcatcaatga ctatcctgtc tcttgaattg
    14281 gagttgtcag cttgttcgat ccgggctttt gaacggatct ttataattcc tcttcctgtt
    14341 tcgtatgctg ttcttatgcc tgttctgccg aggatagtgg ctcctgtagg aaaatcaggt
    14401 cccgaaacat gctgcataag atcgacaact gtcgcttccg gatcgtcaat aaggtgaatt
    14461 acagcattta ttatctctac aatgttatgc ggcggaatgt tgcttgccat acccactgca
    14521 ataccgcttg taccgttaat gagaagtgtc ggtattctcg tcggcatgac ttccggttct
    14581 ttcaaactgc cgtcatagtt atcacgcatg tttaccgtgt ctttatctat atctgcaagg
    14641 atctcgcctg cgatctttgc cattcttgct tcggtgtaac gcattgcggc agcactgtcg
    14701 ccatcgatag atccgaagtt tccctgacca tcgacaagaa catatctcat tgaaaaggtc
    14761 tgtgccatcc tgacaagagt tccataaacc gcctgatcac cgtggggatg atatttacct
    14821 attgtgtctc cgacgatcct tgctgatttc ttatacgaag aattccaagt gtttccaagc
    14881 tctttcatcg aaaaaagaac ccggcggtga accggcttaa gcccgtccct gacatcaggc
    14941 agagaacgat ccattattac gctcatcgca tagtctatga aagcagtttt catggactgt
    15001 tctatcgcga tatcctttat ctttccatgt attatttctg tcatatttat cccccttcct
    15061 aaatatctat tgttgcgtta agggcgtttt cttctataaa acggcgtctc ggctcaacat
    15121 tttcacccat aagaacatcg aagatcctgt ttgcctcggc agcgtcttca gctcttattc
    15181 tcagaagaac tctgttttca ggattcattg tagtttccca gagctgttcg ggattcattt
    15241 ctccaagacc cttgtatcgc tgaattttct gaccctctct tgctataaca agaatttcgt
    15301 tcaaaagatt tttccagccc gatactgtta attcttcatc tctggtcgta tttttcagga
    15361 catagtcgca gcaaccggct tcaagaatct tttccttaag ggagttaatc agttttattt
    15421 ctgaattatt aacatattta tagtcgacca tcgtcacttt atcgacaccg ttgacagtag
    15481 tgtggatctt cattttgaac tcgccggcat aacgggtgtt ctcttcaagc tccattctga
    15541 acggcgcttt gatcgggtga ttggtttcaa gataggttct tatggtttcc catttttccc
    15601 acagagcggt ttcggattta aaatcatcga cattaaatct ggttgttgcg gcaattgctt
    15661 caacaaccga agagtcataa ataaattcag tttttgtgag gttgtcgtta agttcgacga
    15721 gcatttcaac aaacttttta aaatcttttc cggaaagaac cttgtccccg atggtaagcg
    15781 aaagtgactg tgcacctgaa tcgacaagat actgctgaaa ttctttttca tctttgacat
    15841 atgttatttt cttgtttttt gtaataccgt acaatggcgg ctgagcgaca tagacatatc
    15901 ctttctcaat catttcaggc atatatctat aaaaaagagt cagaatgagt gtcattatgt
    15961 gagcaccgtc gacatcggca tctgtcatta ttatgatctt atgatatcta agtttagcaa
    16021 tgttgaactc atctttgcct attcctgttc ccatcgcggc aattattgtc agaattgatt
    16081 cgcttttcaa cagtttatcc agccgcgcct tttcaacatt gagaatttta cctctaagag
    16141 ggagaattgc ctgatttgat ctgtctcgac cctgttttgc agaacctccc gcagaatctc
    16201 cctcgacaag ataaagctct gattttgcag gatctttttc ctgacagtca gcaagtttcc
    16261 cgggaagagt tgtattttca agagcacctt ttcttcttgt catttcacgg gcttttcttg
    16321 ccgcttccct ggccactgcg gcttccatga tcttgtctat gataattcct gcaatatccg
    16381 gattttcttc aagatatgta gtaagagatt cagaaatggc attcgataca aaagtctgga
    16441 tttcactgct cacaagctta tcctttgtct gtgaactgaa cttcggatcg gccattttaa
    16501 ctgaaaggac tgcagtaaga ccttctctta tgtcatcacc gtttatggcg atctttgcat
    16561 ttttccggtt ttcagccaca tagttctgga aaacttttgt aatgccaagc ttgaaacctg
    16621 aaagatgggt tccgccatcc ctgttgaaaa tattattggt atagcagagt gtctgttcct
    16681 gatatgaagt tgtccattgc aaagcgacat ccatcgtaag gttgccctgg tctttgtgcc
    16741 tggaaacagt aataatttca ggatgaagtc ccggacgact ctcgttaaga tagctgacga
    16801 actcttttat tccgccatca tagtggaact caaattcctg tccaaccctt tcgtcgagca
    16861 gataaatctt gacgccgctg ttaagaaaag ccatttccct gagtcttttt accactctag
    16921 gtagtgagaa ttcagtcatt gtaaatattt gcgggtcggg cttgaaagtt atctttgtcc
    16981 ctttttcaga tgtttcaccg atccttttca gtggagcgac aggaacacct cttttgtatt
    17041 cctgataata ttcaccgccc tgtcttttta tcttcagcgt cagccattca gacaaagcgt
    17101 tgacacagct tacacctact ccgtgaagac cgccggcaac tttatatgca ttgttgtcaa
    17161 attttcctcc ggcatggaga acggtcatta tgacttcagc ggcggaacgc ttctcctctt
    17221 tgtgaatatc aacagggatt cctctgccgt tgtctttcac ggtgatgctt tcatcaacat
    17281 ggatatagac cgagatttta tcacagtgac ctgccaatgc ctcatcgaca ctgttgtcaa
    17341 ctacttcgta tatcatgtgg tgcagaccgt ttccgtcatc tgtgtcaccg atatacattc
    17401 ccggcctttt tctcacagct tcaagtcctt tcaaaacctg aatactggcg gcgccgtatt
    17461 ctttttcttc ctgcaactct aatgcaagtt tttcatttac atctgtcata agcaaacctc
    17521 caaagttcta cagacagttc attataaata tcgaatccgg aaaagcaaat ttaatagaag
    17581 aaaaaaacac tatttttttt aggtatatat atttttttag gtatttttta taataataca
    17641 tctaattgta attgcgaatg atttcattta aagatgttgc agtccctgaa aatcggattc
    17701 agaacagaat atcagcgata atttccgcgg cttcagttat ggagtcggca ttttcatccc
    17761 ctttttcaag ggatattttt ctggcgaaga gcgctttgat gacagattcg gccttccctg
    17821 tctcccacag atatgaaaga acatcctcag ccagcttttt atcagatatt tccctgtttt
    17881 tgccaagact cgatctcatg gccccttcaa ttattatttt tgactcgtca tcagagagat
    17941 cttctttaat gatataactg accgcaaagg ccagatctgc tatccatttt gcaccactgt
    18001 atactttgac ctctcctttt tcaattttga aaggcaggtg gaatgagtca gggcactgct
    18061 ccactatctt gacactttca ttgaaaatag tgtctgcggg atctgcatcc tttaattttt
    18121 ccagccattc gcctctttgg ttaagatact cacaggtttc agagttatct gagtaaacga
    18181 ggtctccggg atttgcaatg aaaactatcc ggtcaacctg atttatattt tcaagaaaga
    18241 acgatgcaaa aacttttttt tcgctttgtg caaccgaacc attgacaggg aacggcctca
    18301 tatcttttac catgccgatc tcacggagtt tcacataatc attgaacgga ggtatgttcc
    18361 tgtcactcct taaatagaaa tagaatatct ccgtaaaact ctgcaaaagc ccttgattct
    18421 tcattataat aaaggagttg aacagatcat tgatataagt atcatcatcc cagtcaatgc
    18481 ctccctgaat atttgatatt atataatgaa atgtccttac aagtatttct ccgtcaatgt
    18541 tctttgaacg attgtcttcc atcacatatg cggcaatata agaaagtgct gaaaagaagt
    18601 tgatcgaaag atcttttaca cttaccggct ctgaatcgtt tgaaagatga agttttttct
    18661 tctcccctga attttcgctc tctttaattc cgccaacaat caagttattg aattcattgt
    18721 agaaaagtcc ttccgatccc tgtatggaac catttgatgt catcaacatt agaaccatca
    18781 gggcaggaaa gttttcattc cttctcagat aatacaagta tctataaata ttaatgtctt
    18841 tggcattatc aaacatatcc agatattttt ttaacggatc tgtcccattc aaataaaccg
    18901 tgttcgctgt gtcaaacgaa aaacagccgg gagattcaag aaaaaagatt tcaggaaaga
    18961 tgatcgacgg ctcacatctc tcttccgcat caaacgcaaa cagaggtgct gtaaaaataa
    19021 atgtcagcag aaacagcaga tctatctttt tcaaagtttc tccatcgctg aaaattccat
    19081 gtcggctatc attttcacaa agtctatcga cgggtccttt aaactttttg aaaggaagaa
    19141 atcggtcatg ccccttttct tctgatgcat gtcaagtatc ctttcttcaa ctgttccctt
    19201 agaaaaaagc ttgttgacga tgaccttttt ctcctgaccg atcctgtgtg ctcttgacca
    19261 cgcctgttct tctgtcgcag gattccacca cggatcaact ataatgacat tatcggctcc
    19321 tgtcaggttc aatccgacac cacccacttt caagctgaga agaaaaactt ccttttcccc
    19381 tttgttaaaa ctttcaacca tctctattct gttctgagtg gttccatcca tataaaaata
    19441 atctcttccc tgaaaatcga acgcacttgc gattatttta agcattttca catactggct
    19501 gaagatgaga actcttccac cgccttgaat aatttcatcg gtaagttcaa gtatggcggg
    19561 aatttttgat gaaagtgccg gatcggtgtc tgtttttgag accagtgcag ggtgatccgc
    19621 cgcaagtctg agattggaaa gtatggataa cacctcaatt ttatttatag aatcaccctt
    19681 ctctataaat tctgccctgc ctcttagaag aactgacaga tatatctcct tctgtttagg
    19741 tgtcatttca acaggatatt cctttattat cagttccggc aattcggaaa cagtctcttt
    19801 ttttgttctt cttaaaatga acggtgacgc catacttctt attctttcca gaccctcatt
    19861 ttcgttgttc ttttcacttc tgtcgacatc tttcctgctc cccagatatc ccggcataat
    19921 aaactggaaa atgctccaca ggtcacttaa atggttttca agaggggttc cggtaagagc
    19981 aaatctgtgt tcggtcttta acgaagaaag tgttttgaac ctctttgtat tgttgttctt
    20041 tatatgctgt gcctcgtcaa gaactaccgt tttaaagagc ttatccttta agtatttgaa
    20101 atcattgatc acaacagcat aagatgtaat tataagttcg tgttccgctt tcttccagcg
    20161 cgataccctc tcttccggtt tgagagaatc aatgaccact cttttcattt ctggaaagaa
    20221 tttttctatt tccctgtccc agctccatat aagcgtctta ggacacacga caagagacgg
    20281 tttgtcatca ttttcagatt ttatcatggc aagcgactga agtgtttttc ccagacccat
    20341 ttcatctgca agaatgccgc ccagtcccag ttctctaaga agcatcatcc atttataacc
    20401 ttcaatctga taatttctta tcggtatgcc ggaaaggtgt tccgtcgaaa catccgggag
    20461 ctctcctttt gaaagagaat tgatgatctg cagatacttc tgctgtgaat catcgtcacc
    20521 tgtaaaactg ttaagtattt tcctgttctt tgttttcaga agttttaaaa gatggacagt
    20581 tgaaatttta tcttttgacc tgaacaattc ggaaaatgaa aattccgcta tcttttttaa
    20641 aaagccggaa cttttttcaa gtattaccgg cattccgttc atatcagcga caaccggctc
    20701 ggcaatccct ttctcatact gcataacagc actgacaagt tcggcagaac ttataagatt
    20761 ggcactttca ggaagtttga tattaaaaga aaaccacttc tcttttgaat tgatatcaag
    20821 gactatttct gttttgtccg cagtctcttc atcaaaagag atccttttta tttcctttct
    20881 tgaaacgacc atgcctgcag aattaagagc actgtctttt ttaataatac cggagaaggc
    20941 cgacatcggc gcctgataac agttcctacc ctgacggaaa ccttcgctgc tgagagcatt
    21001 cctgatcttt ttaataagat ccggtgaaat agagtatact cttccgttga gagggtaatt
    21061 ctggtcatag ttctttcttt taggcatgaa ttcgatcttt tcaccggagt gaatgaaaaa
    21121 tatcgaaaca cagactttgc cgttttcaat gtaagtgtca aagagaacat tcgttttgtc
    21181 atcaagctct attttctgca aattcagctc gccattgagt gaaacaaaag gggtcaagtt
    21241 gtcagccaga tcagcaattg ctttaaccag atcatctttt ctgctgtatc ccatggatat
    21301 gttaagcagc gtagaaaccg tttcgttcac acttgcagga agccgccata catttcctgt
    21361 ttctgaattg acagcaaccc cttcgtggtc aaaataatga atgtggtcag aaaaaaagaa
    21421 cctgaccgct tcattgtcga catcataaaa aagggtcagc ggtgaaactg acggggaaag
    21481 cagtgtgttc ccgagcatga atttttcagg aaaaagagag acaagaaata tcagattttt
    21541 ccctccgaag atctcctctg tctttttcaa ctgatagtct cctgcatcat aaagaacttt
    21601 cacatccttt ttattgatgg tgatcttcaa aggaaaatct gtcctggtct gaatgttttt
    21661 tctgccgagc actgaaagct gttctgatat atcggcattt tccattgaaa gctcgttgaa
    21721 aagaaggccc atcgcaacga tatgtctgca aacgccctta catccatttg aacatgttgc
    21781 ggagacaatg cgaccagtac tgtcaattga aaaaagaacg gctgtatgct ccctgttcct
    21841 gatctttcct gatatcttga aaccgctttc acttttttcc actgaaagga ttatggcaga
    21901 tctttcatga tacagggaac ttccttcgag gaattcacac gaaaggacaa gctcagacag
    21961 ttgaacagtt ctttccattt ttacggagtt agaaatttga aacttcataa ccctgaggat
    22021 agacaggggc gaactgtttc ttttcaagtt tgggattata tttgatattg ctgaatttaa
    22081 actgatttat gttcttaagc tgatcaatga tcacgacgct ctcgatctcg tctttgacag
    22141 aagttatgaa tatgtatttt acattaccca tatctttttt cggtgtaagt ttgtaaagca
    22201 tcgatccttc ctttatctct attccggtct tttttgcctt ttcgatcgaa tttgttgagt
    22261 cactgatgat aaattttttt ctgacctcat caagaccctg aagaaatgcc agatattcac
    22321 cctcatcccc tgctgatttc ttgacaagaa tgtttttctt ggagtgataa atgaaaactg
    22381 ttttttcgct gttcagaaaa gtctgctctg ttaatttccc ttcaaaaaaa gtgtccattc
    22441 ttatctcagc gggagctatg aaaaggatcg taccgttatc tgtactcttt tttcctgtgg
    22501 agttctgggt gaaaaccctg ttataatcgg atgaaaagct ttttataccg ttgtactttc
    22561 cgagtagttt ttcaaccgcg gcatcctggg cataaacagt tgacacaaac aaaataagaa
    22621 gaactgtctg cagaaatttc atgtaaacct caatattatt tgtaaatttt taaaatttta
    22681 cttaccggaa tgtcatactc aatgactctt ctggaaagtg gtgagaaaag aagaactttg
    22741 cctgatgcag ggttcgccat gaatgtgaat gcatcgggag agctgaaacc tgcaaacgga
    22801 acgccaggac taagtttgat aaagaatgct gtttcccttt cggtgacaac atcatcggtt
    22861 ttccttttta ttgatattcc ggcatattcg tcggaaaatg agagcacact ctcattgctt
    22921 ccggcagaga gctcaacccc tttccctgca aattccggac cgtagtaact ttcacggctg
    22981 acagaatacc tttctttggg aaaaatggca tagctgaagg gactgccgta acatttttct
    23041 atctggtcct tatacttatc gatctttaca gccagttgac tggaagaaat gacatctgtt
    23101 atgggaaaaa cagcattttc gttccatgga attacacaat caaccgaatt aatacggttt
    23161 aaaagagtta tcgaatcagt caaaggatca attgaaaact tttcaaaatc ggcagtttca
    23221 tcagaaagaa ccgttccgcc ctcgtcaagc tttccgtttc ttgagaaagt atcttcgata
    23281 ataccttcat aaataaattt gtatgtgatc gaatgcggca gacccctcac cgcataaaca
    23341 gatgaaatga acggagcatt ggatggctct gtccgcccgt tattaggata tgtggtgtat
    23401 gtgctgtcaa tgtaaggcct catttcgttg taatagtcag gatttgacag ttcttcactt
    23461 tcagaatact taacaactat ccagcttgaa tttttcatat cataggccat tacatttccc
    23521 tgtttagaag ctatccagag aacatcgccc tccgatgcat caagaattgt ctctccggaa
    23581 ttatctgagt cttcatcagg cgtctcatca tcatccgttt caacatcttc gatcaaagta
    23641 tcgctgtcat cgacttcctc ttttttatat atctcaacga attcattcat ccgttcccct
    23701 gagaactctc tgtattcgaa tgtctggtca tattttacgc tcgtaacgga tgaaatttcg
    23761 ttgccatcaa acattttcag agatttaatt gtatcaagcg aggaatttac aacacggata
    23821 tcggttttat tgtaaaaaac tatcctgttc tgaccgatcg atgtcattcc ggcaccgaca
    23881 agctcaggat ctgtaagctt cacagcgtat ttcagtgaac tgtccgtttc ataaacactc
    23941 cctgacaaac tgtctaaaat ataagcccta tctcctgcga cagcaatatc tgaaacagta
    24001 aaaccaacct cttcatagtc acctttgccg gcaatatctg aaaaaccaag atagtttctg
    24061 ccgaatcttg aaccgctgaa gaagaaccct tcccctttat acgccttcac aattgacgga
    24121 tagcaatcaa gaagaatact cctattgaaa gaagcgtcat gcttcatatc gacacccatc
    24181 tttctgaaaa cgatcctgcc tgcatgtttt tcaagccgga gttcatcatc cttgtttttc
    24241 caatgttcga attcagtata gcccgatgaa gccgtcgcaa agataacttc accatcaacg
    24301 attatctggt cggaagacac acctgtgccg tttatataaa tacctgtttt gtcatcaagt
    24361 tcatctttga aatccttatc tatctttgat gaacaaccgc ttactttcat aagtcgcgca
    24421 ccgtatttct gagtgttgtc aacaacgaaa cccatgtagg aaaccgttcc gttaagacac
    24481 ttggaaccct taagtaccga gaaagacgac ggttgttcaa aaaagtaatc ttcagatgat
    24541 gaatcacatg cagaaagtaa aacgagaaaa acaacggctg gaatgagttt aaaaattctt
    24601 gccgtcatta ttttattcct ccaatctctt cgtacagatc tttgttgtca atatcgatac
    24661 cgtaaagata gaccgtatcc tgtgcaaaac aggaaacgag tacaatccct gtggtgttgc
    24721 cggtgcttga agcactgacc tgataaggtt cacatttgtc aaattttatc tgatcaagta
    24781 tttcatctct tcccgaatca acaatgtaga ggatcttctg tccgggagat gtgatgaata
    24841 tcaattctcc gttatattca ttttcatcac ttccagtcac atacggaagc actgatattt
    24901 ctcccagact cccctttaaa gcaattgttt caacaagttc agtttcagtg ctctttttgc
    24961 cggcaagctt aaactttaaa agctgcggcg ctgaatcttc atcactgtcg tcggaataat
    25021 tctgaaaaga ggcatagaaa gaaacaccat cagatgaagc tttaagtgac cggatatcat
    25081 atccgtctgt cggaaggtct atttccgttt ctgtgatccc gacattaaag ccgcttaaag
    25141 attcagacgg ctcaataaga gtaatattcc ctgaacttct atgggaagcg agataatatc
    25201 ccgacacagc atcgtaagct atatcagtga caccgtattt taacttgtgt gtctctaaca
    25261 gctcccattt ttcaagatca actatggaaa actccccgtt aagaaggtgt gtaacaaaaa
    25321 gtttgtcttc atcctcgtta agtatgatgg cataaggttc cttctgtgtt tcaagtattt
    25381 caggaatatc tccgtcggtg tcatctatat agctcaaacg gtaagcgccc gccttgcctg
    25441 agatcttaac ccttacaata ccgtgacggt ccctggatgt tacataaacc atgctttctt
    25501 cactgttgac agccatttta cccgcaagtg aaggaactat tattgagtcg atgtatgtca
    25561 tttcgccctc gtcatcgatc tccattatga caatcgaccc gaaatcatat tttctgttca
    25621 tgttcgagtt aagatacata aaagttgtgc cgtccttaaa caagtagaag tctgtgacac
    25681 ggttcacaac atcttcagga ggatagataa gctttgtttc atcactgcat cccagaatgg
    25741 ttataagaag aaatgccgtc gcaattattc tgaatggtgc tgtactcatt ttttatcccc
    25801 ctttttcgca tctttttcat tctttccggc cggtgttttc ttcttcgcgg cattgcctga
    25861 gatcattttt tttaaatctt ttatctgctc cgcaaacgct gatttcgaaa attttatctg
    25921 catagcttca agcacttttg aagcgacatc ctctttcccg atgtagaaaa gggcatacgc
    25981 agtcctgata tagcttgctt caagctttga acaggtattg aattcactct ctatcttacc
    26041 gaaatattca atcgcattgt tgtacgattt ttcaaggaaa taactctccc cggtccagta
    26101 tgccacatca catctcctgt tcttcggcca tttcagagaa tagacatccc atccttttct
    26161 tgcaactgag ccgtcacttg cacttcttgt aaaatcaaca agctgattgt agcttttttc
    26221 ctcattttcc gggagtctca ctacaccgtt ttctgtaact gtaaggtttt caacaggttt
    26281 cagctcgctt atctgcatga gcatgtcact cttcaatttc tccagttgct cttcatataa
    26341 cttaacggct ttttcaagct ctgaaacttt tgatgaaagt ccgcctccct gctgagccgc
    26401 gatatctaca ctatcaatcc tgttgttaat atctcttatg gtcgacttaa gctcatcgag
    26461 agtgttaaaa gaaaggttga tcttattctc ctgggctttc ttgtctatct gcaaagtctt
    26521 ttctatttca tcaagccgca gtgacatctt tgctatttca gatctcaaag attcctcaag
    26581 aacttttatt tttgcattat tctccgcagt aagctgcgat gatgccatat ctacatcctt
    26641 tttcaatgcg atacatgaat tcatcaatga tatcaacaaa ataaccgcga gcacctgaaa
    26701 taatctcatc ttgtctccat aactcttaaa accatttacg cggtataata tgcttaaatt
    26761 ttcacataat caatattttt atgcattaca gaacactatg agatgtgttt gtaaaactat
    26821 tttacagctc gttgccctgt cacccctgtt tgtaaaatcc cttttaagca ggattaagca
    26881 ttaatctaat aaaaacagag agttgcgcgg gcacactttt tgcttataat attagttttt
    26941 ttgttggttg ataatccaca ggtttttgta aaatgaaaga aaatctttcc tgcttaaata
    27001 ccggcagacc gctggtcagt tctcttatta aagatgttgt tgcttacaat cccattacga
    27061 gcagagacaa aatcctttca aaacagacat attgcgatct gccgccattg aaacttgact
    27121 ggaatgagtc aactcttcca ccttcaacca cagtaaaaaa cgctcttgtc gaagcactcg
    27181 cctgcgaaga aaatctttta aactggtatc ctgaactgtt cagcaaggat ctacggcaga
    27241 agattggaga atactgcaac aggcaaatgg aagaagtgct tgtaacaaac ggaagtgacg
    27301 atgcgcttga tcttatatgt aaagtgtttc ttgagccggg agacgaagtt gtcattccat
    27361 atcccacata tacccatttt gtgacatatg ttcagtcaag aggagcggtt cttaaaaaag
    27421 ttgaagcacc ggaccctttc aaaccggaca ttgcctcaat aattgaaaat ctttctcctg
    27481 cgacaaaact catctatgtt gtaaacccga ataatcccac gggggttctt atttcaaaaa
    27541 agcagatatc aactctctgc tctgttgcaa ggaacgccat cgtgatagtt gatgaggcct
    27601 attttgaatt tgccggagaa acagtgatgg atctcatcga cagacactcg aatttaatta
    27661 ttacaagaac tttttcaaaa gcgtgggctc tggcagggtt gagagtcgga tatcttgtaa
    27721 cgaacattat tctcattgat gaactgagca aggtgataaa ccctaaaagt gtaagcgttc
    27781 ttgcacagat agccgccgct gccgcgcttt ctgataaaaa atatatggac gactatgtta
    27841 tcgaagtaaa aaacagcaaa atgatgctcc ttcagttttt cagatcacgg aacatagtag
    27901 cccatgacag ttcggcaaat tatataatgg tacagcatcc caaactcccc agatttattg
    27961 aagaaatgga aaaagagaat gtttttgtaa gggacagaag ctcttttaag aaccttcccg
    28021 gctttttcag aataactctc ggtgatcaca ttcagaccga tgatcttata acaaggttgg
    28081 acaaagcact ttcaagggtc tgaatatctg ttatttacat ttttatggaa aaaatggaat
    28141 atttagttag attctgctgt ttgtatgccg taagatgttt tttgcagatt aggaaaagat
    28201 ggaaaaacta ttaaaagaca atcttgaaac tattttcagt tactcccttt ttgttaccgg
    28261 caacagggat aaggcacttg atctcatgca ggatgcgatc gtaacggcct taagtaagaa
    28321 acatctttat aaagaggagt ctcatttcag gtcatggata ttcagaatac tgaagaacac
    28381 gcacctaaat aacataaaaa gagcgcagat caagaaagaa tcatatctgg ctgatatttc
    28441 aaaggaaaat gaggaaacac cctcatttct gatggcatct ctgactacac ttgaggaaat
    28501 gagcgatcct ctgctgaatc aaaaaatgag tcaagtgctg gaaataatgc ctgctgagta
    28561 taaggatgcc ataatactga aagaaataga aggtctttcg tatgaagaga cagctaagca
    28621 gcttcaaata ccagtaggca cagtaatgag cagacttcac agagccagag gttatttgaa
    28681 aaaagctttc agaaaagaag cggtcgaatt aaagatagtt acagaaaggg tgaaaaaaaa
    28741 tggttgaaac aaaatgtgac atgaatgaac ttttcattga acagtatgca gacaacgaac
    28801 tcgatctgcg tgaaagttct gaagtttcta ttcacctggg aagctgtgtt tcatgtaaaa
    28861 agagatatga agagatcatt ttcgcaaaaa atgcggtcaa caggtttaat tcacttaaca
    28921 agctttctct tattgaaaaa gagggttttt acagcttcat tgatcaacat tcttcaaaat
    28981 acgggatcgt acacaaattt tttgaatatc tgcgcagtca cggctttact gtagcggttt
    29041 ctacagtttc attcacttca cttgtgtttg catttgtatt ttcactttca cagatagaaa
    29101 aagaaaacaa cctcatcata aaagagatca tcgcggcgca caacaatctt cttccctatg
    29161 attttgacac acccgaaaag accgaaacag agttgaataa aaaattcacc atcgacaaaa
    29221 aaactttgct taatcttgca tcaatatcgc ctgttttaag gggtcgattc acatcaattg
    29281 ccgcaacccc ggctgcaaaa ataagcatta aaggtttgaa agaagaggaa aagggaacac
    29341 tttttctttc aaaaaacaat gctcatataa aaaatgtttt tgaagatgga acctgtctcg
    29401 ttaagcatcc cgacaataat tgcaaagcaa agctcatcca gaagcaggga aatgatatga
    29461 tccactggga ggagcttgac aatgattttg tttttgtaac tgataacaac agaatgagtg
    29521 ctcaaatggt ccaactgata agtaattact gatcagttgt ctatatcctg tatctttgtg
    29581 acttcctttc tcaggtttgc cgtttcaaca ttgagttttc ttgaaacctg atacacatta
    29641 tccaccattt ctgtgctttc ctgtgtgatt cttctgatgt cactaagcga tctgtcgaca
    29701 cttcccactt cattcacatg attttctatt attcctttta tctggcttat ctgattggaa
    29761 atactgcgga attctccgac aagctcattg atgaaactga cgatgttgtt tactgaactt
    29821 cttccggaaa cgactttgag attggtatca tctataagtt tcttgatcat ttcgacagca
    29881 tttgctgtct gcattgaaag ctcccttaca gattccgcaa caaccgcaaa accttttccc
    29941 gactcccctg cccttgccgc ttctatcgcc gcattaagtg acagaagatt cgttttaaag
    30001 gcgatctcgt caactacttc aactatgttt gaaacctgat tagcactgga ttcaattttt
    30061 tctattgaat caactgcctc gccgagaaca atagttgaat ggtccgcttt ttttgaaatt
    30121 tttgtcagaa cctgactcat atcctgaact tcattccttg tgtcggaaag ttttgatgta
    30181 atgtctgaaa gagttgatga cgcagtatct atcattgcgt aagtcgcatt gaatttttcc
    30241 gctgttatgc cggtgttgct tgaaatgagc tttgagatat cggcaagtgt ttctgacaga
    30301 gttttcacat gcagaatgat atccttaaga ttttttgtcc tcgactggat agtcttactc
    30361 aggacatcct ctcttgatct tatctgaacg acctttcttt ttatccagat cacgagaaat
    30421 gttaaaaatg ctatgacaag aagcgtcttg aaccataatg tctgccagaa aaaaggttca
    30481 attctgaatt ttagagaagt gccttcggtg ttccagacat tatcattgtt tgctgcaatg
    30541 accttgaatg tatagtcacc gggttcaagg ttcgtgtaaa aagtatacct tttcccctca
    30601 gcttctttcc atttgtcatc aaatccgtcg agcatatatt taaatgaaac ctgatcgggg
    30661 aaaagatagc tgagcgcagt aaattcaaat gaaacagttg ctgtgcctgc ctcgaaaatt
    30721 attttctcat caggcattac ctgataaatg acatcatctc ctttcacaga tacgatatga
    30781 acaggaggaa caacaccgtt aattatagtg tcagacggat caacaacggc cgcaccttcg
    30841 gcagtgggaa tccatatttt accatcactc gatcgccata tgttcggctg ggttcctccg
    30901 ttgcattcag gggtcagcaa tccatcctta aatccgtaag ttttgggggt tacgacagga
    30961 actttgcctt gaagaaaatc ttcgagatct cttttttcta tatacttgat ccctttgttt
    31021 gaagtcagcc agagacctcc cgtcctgtct tcgatgacat caaagatctt tctggtatca
    31081 ataccttttt tccggtcaat tgtaatcgcc cccatgcttt tttcataaac tgtaagcccg
    31141 ttatcggttg caaaaatgat cttgccgtct gatgccggat atactgacag cgtggcgata
    31201 ttttcaaggt tcgtgaaatc tttcacaaga gtttcgccct gtattctgaa agcaccgttg
    31261 tgagttccta tccagatatt tcctgatctg tcctcggcaa ttgacgagat attcattcca
    31321 tcagttcccg ggaaaagttt gaacggagga ttttcttcca tgtaaagaag acctttcgtc
    31381 tgtgttccga cccaaagaac tcctcttgaa tccttgaaaa tagccggtat gagcttttct
    31441 atcacaccgt cagaatcaga atagagacgc gtttcattat catcaatttt aagtattccc
    31501 ttgccgtaga cgctggccca cagagggccg ttcttttcct gaaacagaga aagtatcgtg
    31561 tcatatttag caaggtggtc accgaaagta tactgcttta tcttgttatc tttcagttta
    31621 taaagagctc cgccccaggt gcctatccac attccgccgt cagaactttc agttatcgga
    31681 aatattacgg gctccgaaag accgttcttc cttccataag tgatgaactt gccttccttc
    31741 aacctgtgaa gggcagaacc ccttgttcct atccataaac ttccttcatg atcggaaatt
    31801 attgcaactg catgatttat gggcagccag tttgacttac tgtcctcttc attgcaaatt
    31861 ctgaaaaatc catcattcag agtgcccaca taaacacatt tgtgggaatc aagataaata
    31921 gcactgacca tcttgtcccc gaactgattt ttcttatacg gaaaaacatc tccggtcatc
    31981 gggttgaaac tgtatattcc gccacccatt gttcctatta taaaattttt gggactttca
    32041 aaatagacag tgttaatttc gtaactcagc ttcagaactt cctttgcagt agacccttct
    32101 attttcgaaa gatagttgta agtggcgacc cagaccgtat catcctttgt cttgccgatg
    32161 aatttgacat tattatttgg aagtcccgct gatttatcta tcagtgagat actttcatcc
    32221 ggttttatga gaaccactcc tgaccttgca gtgcccagat agagcatgcc gttgagagtc
    32281 tgcacaccgt acttgactgc gttctcaggg agaccgtttt ttgtatcata gacagtgaat
    32341 ttatcgtttt cataattaag cttcacaaga ccgccgccgt tgaaaccgac ccacaagtga
    32401 ggagttttgt tgtcctcaat gataaatccc gtgttgtgtg tttcgatcgg aagaactgaa
    32461 tcatcataga gtttgaattt cagcccgtca aacttgacaa aaccctcttc agttcccatc
    32521 cagagatatc cgtccctggt ctgtgttatg caagttacgc tgttctgagg caaacccgag
    32581 gaattgtccc atttatcgat catgaactgg ttaagtgaaa catcttttga aagtccgctc
    32641 acagttatcg ttgaaataaa aataaaaact gaaataaatc ttttcacggc acgcacctgt
    32701 caataattaa actgcaaaat agtatgaaaa aatgaattaa atctcaagtt atttatattt
    32761 tcttataata tcagggaatc tatctgaaaa aatgacggcc gtcgccgctg tcaacattaa
    32821 aagacctgca aagaaatgtg ctgacagtgt ctcattgaga aagattgccg aaaaaagaac
    32881 tgcaagagcc ggcttgagca tgaaaatgat cgaagaactt accgctgtaa atcttttgat
    32941 tgtttcaaag aatgtaacat accccgcccc cgtaacgaca aaacccagat aaagaaatgc
    33001 caatgttctg gaaacattaa agaattcagc agaaggaacg aaagatatat ctgccgctga
    33061 cataaaaata aagtttgcag taagacccga aaagaatgat gtcagattta ccgcaaaagg
    33121 gtcgatcttg gaaacggtac ttttgcttag aacagtgtat gccgcaaaca cgatagctgc
    33181 ggaaagagcg aaaagtgcac ccgaattgat aataaaccct ttttctgcca taataaaagc
    33241 aaccgcacct acaccggtga atattccgat acttttccta agcgaaaatt tctctaaccc
    33301 cagaacactt gatatgagca tgacaaaaac cggattcgag cagaaaatcg ttgccgatgt
    33361 tgccgcagag cagtgcttta cagatatctg aagtatgctc atggcaagga aagtatttaa
    33421 tattcctaaa gaaattacag ccagccagtc ttttttatta aaatgcatga tctctttaaa
    33481 tctttttttc atccccggat agaataaaaa gaacaaaaac cctgctgcaa aacgccagaa
    33541 agtaagagca aacggatcaa tataccccat caaaggtttt gaaactgctt ccatcgaagc
    33601 aaaaattatg agagttaaaa taaaatagag catatatcct tatcaaaaaa aacaatctga
    33661 tacggtgata tatcatttta tgtaaaagtc aatgttagct ctgagagcaa aaatattgac
    33721 aatcatcaga cagattgtac aatcggctgt caatttgccg gagtggtgaa attggtagac
    33781 gcactggact caaaatccag cgggggcaac tccatgccgg ttcgatcccg gcctccggca
    33841 ccacctcaaa aaagcttttt atttttttag tttatcaaaa tgttgatttt taatactctc
    33901 ttattaaaat cccccctgtt atttttttgg aatctttttt gtggggttta taaatgtttc
    33961 aagacatcag aaaaaaaagc agttcgattc tcgtatctct tatgtttgcc gccatcatcg
    34021 taaccttcgt tatatctttc gggcctggtg atgatgcagg gtgcagttct tcagctcaga
    34081 attatgccgc atcagttaac ggcgaactca taaccacttc agagttccgt ttttcctatt
    34141 cgaacatgta tgattactat cagcggatct tcagggattt caacaatgaa atggccgctc
    34201 agtacaagct ttccgaaaaa gctatggacg gacttatcgg tgaaatactt cttgcacagg
    34261 aggcaaaatc cctcggattc aaagtttccg atgaagaagt gagaaaagag ataatggaaa
    34321 caccgtattt ccagcagaac ggtgcttttg acagggattc atacaacaaa atggttcagt
    34381 tcacacttaa cacaactgtt tcagattacg aacacaaagt gcgtcttcag cttttaagca
    34441 gaaagctcag aagtttcctt ttccagagca ttgatatttc tgaaacagaa gttcttgatg
    34501 aattcacatc atctgatgaa aagatggatg cttttgtcta ttccatactt gaatcttctc
    34561 tcaaggatga aataaagaac aagatcaaag acgagatctc tgatgatgaa gtgaagacat
    34621 tccttgaagc cgatctcaca aaggttaagc tgacatttga agacaatgtt tcaaaataca
    34681 gcaaaaaaga cggaagcggt caaactcttt ttgaagacgt taaagttgat gttgctaaag
    34741 atataatgct gaaagacaag atcgctgcta aaatgaaact tgattctgaa gcagttttta
    34801 ccgcagttaa agaaaatccg tcaataacta ccgaagatat ctcaaagaaa tttcctgact
    34861 ggaacatttc aagaaaagag gttcccggtg tagttaagaa tgcaaaattt gttcagggtg
    34921 tcggattcag cccgaaattt gtcgaaaagc tttttggatc tactgaaccg ggtgtacttg
    34981 acagtgtttt cataactgag gaagggaacc atgtcattgc gggaagaatt aaacatgttc
    35041 cggcagatat ggaaaaattt gaaaaagaca agggaaacat aaaggaaaga cttaaaacta
    35101 caaagaaagc acagatactt gaaaactatg tagaccagtt gagagacaag gccacagttt
    35161 caataaacga gaacttcctt aaaatatact catctcccgc tcaagctgaa tgatctgtaa
    35221 atgaccagtg agaaattcat cactatcctt tccacggcaa tagttttaat aacgatagtt
    35281 ttttcgtcag ttgtttttgt aaaagagctt gttaagccgt ctgatgtgct ggaaaggacc
    35341 ttgctggaca actgccgtga gaacaagttt cctgaagata agatcaatga tgaaaatgaa
    35401 cttgaaaaca ttgcgtctga ctgtgttttc atatcagacg aacttgccga atcatcagaa
    35461 ttcaccaaga aatttaaaaa cttgcagcat ctgaagaaat ttgattatct tttccttggg
    35521 aaaaaaggca tccttctaaa aagaaatacc gttgatcata aaaaagatgc ggaatacata
    35581 tataaatgtt ttaaagacgc cgcatgttcc gaccagggat ctgatgaaag cattaccatt
    35641 acctatttaa tgcaggacag caaaggtgaa gttgcaatat caacaacata taacgatctg
    35701 ccggcgcatc agacagttaa aaattttaaa aaacttcata aagagatctc tgccataaaa
    35761 aatctctctg ctgatcttag acttacgctc ctttttcata aaagttacac attcattgaa
    35821 gataaaacac ctgaaaatgt gatttcactt ctgaaaagga aggttgatgg gctttttctc
    35881 aagtcaagag gagcaaagat cagagtcctt ccgtgggaat attccgataa tcctgtaaat
    35941 gttctggaca gaaaaagccg gcagtacggt cttgacaaag aggaatttaa gaaggatgag
    36001 gcctctgtct atcattacag aactgtccag tttatagaaa agaacggtga aatggtgcca
    36061 tggcttggaa tttacaacga taaaaaaagt aattattccg acaatcttgc catcaggatg
    36121 atttcaaaac atcttaagaa cattcaatcc tccgacggtt cttttcctgc cgagatggat
    36181 atatttgaag gtgctgataa aaaatcaaaa gacaacctta atattcaggc ggaaacagcc
    36241 attgcacttt tcaaagcttc tgccgttcta aaagacggcg ctcttgctga ttcagcagta
    36301 aaagctgtcg gatatcttat aaaaaagaac ctttcggctg aagtttcatc agctcttctt
    36361 ctatatattc tctctctttg cggtgaccag tgctctcttg atgaacatgc tgaaaaaaaa
    36421 gatgagcttg tgaaattttt cagtgacatg gcaaacatca gaaaaagcat agctgataat
    36481 ccggtatatt caggcatttt cctgaacgca ttttcagcat catcagaaaa attaaaagat
    36541 caggaaattg aacttctgaa aaacgctctt gataaatatt ccggactcaa ccactctgac
    36601 aagctgagat tcatttcata tttaatgtgg accgattaca agaaagattc agaaacattt
    36661 gaaatggtta gaagttttgt caaccgcgaa actgtattta ttaaagaaag tcttttcgat
    36721 gaccgcggtt ttaatgattt cagcggaagt tttatgtcat cagcaaaaaa gagtattcct
    36781 gatacagcca tttcagttct tatcgcatca ggtctgtcaa gggctgtttc taaagactac
    36841 cacaacgatt ttatatttga aaccgaatca ctttcgggta attttctaag atttctgatc
    36901 gttacggatg atgatttccc gaactggggc tcatcaaaag cgaaaagttc ggtgcaggga
    36961 ggagtgagag tttttcccgg agcaggaact gtgaagcttg caaacagcac aagagttctt
    37021 gagtattttg tcaacagggc aataaaagga agggcgcgat gaacggattt atctgtaaga
    37081 tctgcggggc atgctgtaag ggcgaaggtt cggtcttttt atatcctgac gatgttaaaa
    37141 ggatcgctga aaacctttca atgacactcc aggagaccat aaataaatat accgaatatg
    37201 ttatgatgga aactattgag aaaagcggat cgtactcata cctccccttt cttatactta
    37261 aaaaaaataa aggggcatgt gttttttcca attcaaatct ttgcgaaata cataaattca
    37321 aacctttcca gtgtatgcac accccttttg tttcagagtt tttctctgat gaatcctggc
    37381 gcaaacagtt gatggccgag tgccctgcat tgggtaaaat gaaaaaagag gatttcgcag
    37441 tgtatgccgc aacaggcgaa atgtctgaaa aagcggagca ggattactat cttctcctga
    37501 aagagaacag ctttaatctc gaaaagatcc tgagtgtgaa actggggaca cctgaaattc
    37561 tgacatatca tgactgatct tacgactaca aaacagaata caatacagga atgggacatt
    37621 atcatgaact ggatcggcga aaaagccatc tcggaatatg gtaaaagatc tcttttcaga
    37681 caggatgcct taagccgggc tgaatcagta aaagaatttg aaatgatcgt ggaggcatcc
    37741 actctttccg aaacatcaaa gggtgttttc tcttttaatc ttgattccgt gaccggtgtt
    37801 gttgaacggt ttgagaagaa cgagccgctt gaagcaaaag actataggat cattggtgat
    37861 tttcttggtt caataagaaa atcgggcgaa gagataaaga aagagaaatg ggcttccgga
    37921 attaatttga tacttcctgc cgattttgac ataagttttg aaatgacgat caagcagtgc
    37981 ataactgaca aaggggaagt cagttcaaat gccacacctg aacttgcaaa aataaggaaa
    38041 cagctttcag acacccataa acacatcggg atcgagatca aaaaaatgat cttaagccct
    38101 gaatacagct catatcttca ggaagactgg ttcacggtaa gagacgacag atatgtgctt
    38161 ccgttcaaat cagttttcaa aagaacaatg aaaggggtca ttcacaacta cagcagaacg
    38221 gggcagacag cgttccttga accgcttcct ctgatcgaac ataacaacgc tctctccctt
    38281 cttgcggcaa aggaacttga agagataatc aaggtcttaa aagagcttcg cggacttgta
    38341 tacagaaaat atgagtatct taaaaaatgt attgaaaccg ccctgcatct tgaaaacatc
    38401 tatacaaagc ataaatggat gaaagaatac ggatgtgtca tacctgaatt tactgatcgt
    38461 gagctgagaa tagagagcgg atggtatccg cctgtgtttc ttactgccgg taagtcactc
    38521 gtgaaaaacg actttatttt taatgatgaa gagaaaataa tggtcgtgag cggacccaat
    38581 gccggaggta aaacagtggc
//
LOCUS       DAOOYB010000005        25294 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_1758_length_25294_cov_10.790324, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000005 DAOOYB010000000
VERSION     DAOOYB010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 25294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25294
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1758_length_25294_cov_10.790324"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="PL056_00690"
     CDS             <1..307
                     /locus_tag="PL056_00690"
                     /inference="COORDINATES: protein motif:HMM:NF014517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HPM45560.1"
                     /translation="WLLGVGSETLKNFGAVSIECAREMAEGLSKRSSADVTFAVTGIA
                     GPDGGSEEKPVGTICFALCHNGTTITELRRFNGDRTNIRTRASIFMLDMIRRAVYLK"
     gene            536..1417
                     /locus_tag="PL056_00695"
     CDS             536..1417
                     /locus_tag="PL056_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HPM45561.1"
                     /translation="MAGNELIKIDDIKNRIITIRDTQVILDRDIAEFFGVETRTLKQA
                     VKRNIEKFPSDFMFELTKDEMEIMVSQFVIPSYKSFGGAKPLAFTEQGAAMLSAVLKS
                     EIATRISIQIMRAFVQMRKFVSHNIKIFNHIELIEKKLLQYSSESDKKFEILFKALEN
                     NEIPAKQGVLFEGQVFEAHKFVSDVVRSAKKSIILIDNYVDDTVLTLFSKREQGVTLK
                     IFTKNLSKQLQLDTQKFNEQFPPAEIKEFNHSHDRFMIIDDKDLYHFGASLKDLGRKW
                     FGFSKMNIEVTKMLLRL"
     gene            1609..2955
                     /locus_tag="PL056_00700"
     CDS             1609..2955
                     /locus_tag="PL056_00700"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HPM45562.1"
                     /translation="MKVRFAWLLVFVFTTFFVSCENSTNSKNDETVNDEAVTDDMSDD
                     LSDEVLTESDETNDEESAEIQLKYKESDEKAEAVENDLVNSNTEFALKMFKALLEADK
                     DKNVFISPVSISVALAMTMNGAADETFDGMMEALEFAGLTLENVNESFLLLLESLEYS
                     DKDVLLTLANSIWIAPAYKPEISESFIESVEKYYLSGVFEATSPEVINKWIEDHTGGH
                     IKDMIKEFPDLLVMYLVNAIYFKGAWTTTFDKDDTYETDFTKEDDSTVKVNMMAFKDM
                     AEYESYVSEKVVGIRLPYGREKISFYAFAPIDWDYQPIDEFINSLDQESFDEIIGSFD
                     KHELGGVHLPKFKLEYEKELNEILKAFGMDKAFSGGFDNMVKVDSSANPYISLVKHKS
                     FIEVSEEGTEAAAATIVEIVDESMPTTFMANRPFFFVIRDDRNGTILFMGKVADPS"
     gene            3029..3940
                     /locus_tag="PL056_00705"
     CDS             3029..3940
                     /locus_tag="PL056_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006313496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HPM45563.1"
                     /translation="MLYLMLAVISSSSIALILKFSESRDLNRLVVTTSNYVVASVVSL
                     FFVLEGGLFNRSAIDVPTFFLEMNVILNGGIFSENSSFIWAIATGIFGGILYFLGFIY
                     IQKSIRDNGVGITGAFSKIGIFIPMIMSIILWKEYPSNIQWIGIVLAVSAIILANYTP
                     EVKNSLFNFRKSLILVFLIAGLAEFSNKFFQNYSIPEYKSLFLLVVFSTALIISSVAT
                     FIERKPFTKKDLLTGFLVGIPNMLTSFFLISSFKYVKATVAFPAYSAGTIVVINLGGY
                     LIFKEKLMKKDMFATAIIVSAIVLMSL"
     gene            complement(3944..4507)
                     /locus_tag="PL056_00710"
     CDS             complement(3944..4507)
                     /locus_tag="PL056_00710"
                     /inference="COORDINATES: protein motif:HMM:NF015074.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45564.1"
                     /translation="MKKLFSFLSIVFALLAGFSACGNTKTDNYTDSEIAKSDSNDEDS
                     GNTSDTGNTGNTEYFDDTIYGIEYCVFIKVSGTVTNEESLPLENIDVHFCEKYYAEEE
                     CDLVSVSDSGGEFEFTKENSTFDINDNLSLKFHDSSGRYSNKDVFIEMECIFDENDGR
                     IYKYDCKNEDVKVVMEETSDDDTDSDI"
     gene            complement(4504..5583)
                     /locus_tag="PL056_00715"
     CDS             complement(4504..5583)
                     /locus_tag="PL056_00715"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPM45565.1"
                     /translation="MQTLRNRTFLPLKFYFDKKVRKLNFIFFELTQKCNLSCVHCGSD
                     CNKDNNSADLPADVILKTLIDVKKKYDSHKITIVLSGGEPTIYPGVWDLGKKIIDLEF
                     PWGMVTNGLSWDEKTIKKAKESRMHSVTVSIDGLEENHNWFRGNPKSFRKAKNALKMI
                     LETPEIYKKDVVTVINRRNLNELDDIYEMLKKMGLKRWRLTGVDPIGRAKDNPDLFLK
                     PDEFVKFMEKIKELRNRREIDLMYACNGYMGPFYENDIRSRPFFCMAGINVAGVMVDG
                     SILACPNIDRKLSQGNIYNDSLVDVWENKFKPFIDRSWKKTDKCSDCSEWKMCEGGPM
                     HLWNYEEKTLSLCHYKDLILKGEKE"
     gene            complement(5601..>6007)
                     /locus_tag="PL056_00720"
     CDS             complement(5601..>6007)
                     /locus_tag="PL056_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45566.1"
                     /translation="GDTAYGVMYSIYYNISGVIVNEENDPLENINVKICPFSYPECKQ
                     VAKSDTKGKFEFTEQEQEADYIPGKVKLELVDPDKEYASQTAEVSLKCELKHCKNNDV
                     KLKMHEWTGDDKDTYESIDSDHPDDTNDDSDQ"
     assembly_gap    6008..6107
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6164..6505)
                     /locus_tag="PL056_00725"
     CDS             complement(6164..6505)
                     /locus_tag="PL056_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPM45567.1"
                     /translation="MFTGWIEKFGAAITVMDLEGNILDMNEAASVVNEKHGGKSLIGK
                     NLMFCHQQRSRDIMKDLMENDTKNVYTIEKNGKKKLIYQAPWYDNDGKVAGLVEISMF
                     VPEETAHYKRN"
     gene            complement(6531..6971)
                     /locus_tag="PL056_00730"
     CDS             complement(6531..6971)
                     /locus_tag="PL056_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45568.1"
                     /translation="MSRYILIIMVIFSVLMSLSCSSSKEKEKAAIEQLIKNNAEFFQK
                     EDLESYIGTLDPTSPEIENTRAMMVELFENYDLKLTIESTRILKMSDNKADVEIVQTT
                     SKVSGGEFRDNRINIVHQLKKIDGAWKITSSKTIKIDYFDQPQE"
     gene            7113..7493
                     /locus_tag="PL056_00735"
     CDS             7113..7493
                     /locus_tag="PL056_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HPM45569.1"
                     /translation="MKKIISTSNAPKAIGPYSQAVEANGLIFISGQLPVDPATGKIVD
                     GDISAQTDMVMKNIGAILEAAGCTFANVVKSTVLLHDIADFKAMNEVYGRYYPENSPA
                     RAAYQVANLPLNAKIEIETIAAKN"
     gene            7512..8690
                     /locus_tag="PL056_00740"
     CDS             7512..8690
                     /locus_tag="PL056_00740"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPM45570.1"
                     /translation="MKFTEFEKEKLILLNEAEYGRNSAYFNWITEEEGTVAQKERDAL
                     VKEIKDLGAQFEFLGSKADSRYLSKGCRICGEGGWSCLFINGMCNANCFYCPSRQDDA
                     VIKPTTNTLEFESPQKYADYVSFFGFKGVSFSGGEPFITFDRTLKYMKELRKQCSPDI
                     YIWLYTNGSLVNQEQLKMLKDEGLNEIRFDIGAFGYSLDKAEMAARHIETVTVEIPLA
                     PDHFETVRDLVVPMKNAGIKHLNLHQLRVTPYNVNKMMDRDYKFVHGTKVTVFESEIN
                     VLKVMLHSLKNGGVPVNYCSFLFKSGFQSSAVRKRYAKLCVQDTEFITQNGYIRNISQ
                     SGSSITVTYFNPVVSGTDFCRIERKKVAVFNSENGFSHKDLHDMSNFEILRGGFADYF
                     "
     gene            complement(8662..9330)
                     /gene="rpe"
                     /locus_tag="PL056_00745"
     CDS             complement(8662..9330)
                     /gene="rpe"
                     /locus_tag="PL056_00745"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018395804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HPM45571.1"
                     /translation="MIKIAPSVLSADFLKLEAEIRSIEESGADLVHLDIMDGNFVPNI
                     TFGPMIVKQIRKVTTLPFDAHLMISNPTLFAPLFVEAGCQMITVHAETETHLHRTLQF
                     IKKLGVKAGISVNPHTSVSFLKYCGDLCDLFLVMSVNPGFGGQSYIESATDKIRKVKE
                     IREKYNFNFEISVDGGVNGETVSKVYEAGADIAVMGSYFFGTPIEKRRELVQNIRNSL
                     QNRL"
     gene            complement(9327..10634)
                     /gene="rsmB"
                     /locus_tag="PL056_00750"
     CDS             complement(9327..10634)
                     /gene="rsmB"
                     /locus_tag="PL056_00750"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF011494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HPM45572.1"
                     /translation="MTFNRVKDPRYTASSLTRTVLDQSLFINELLNDSLSSFEYSQRR
                     FITELVYGTVRNMKHLDFWIFKAFKKPLKRIDPELLSIIRTSLYQIIFMNNRDAALVV
                     NEAAELGKKCGGEKTGGFVNFILREILRLDPSKENMEKFFKKDPDKFLQTFYSVPEWL
                     YRRMVALMPEEPVETYLQIINRPLGITLRVEGDEKTKEKVIANLVSKGAEAIAAKDCR
                     YAIYTSKAVNYDMIKDIAGVFIQDESSQLAVCDMDINKGDTVLDLCAAPGGKTLFAAY
                     LTGNTGKVVGVDINRHRLNLLTEAAVRHKKSNIEVKLHDAGIPKPEWNSSFDKVLIDA
                     PCSALGTIRRHPEIKWLKNDSDPEKMSIQAGKILNTAADYVKKDGTLLFAVCTFTKEE
                     TTDQIQKFLLKHTNFKVEKAYYTVSSIHDNRDTFFICAMRKIK"
     gene            complement(10631..11548)
                     /gene="fmt"
                     /locus_tag="PL056_00755"
     CDS             complement(10631..11548)
                     /gene="fmt"
                     /locus_tag="PL056_00755"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HPM45573.1"
                     /translation="MKTLFMGSPEISVPFLNFLASKNTDMIVFTQKDKIRKRGKALEP
                     THVKKRALELGIPVYTESAKSDEAYKIIKDFGPDIIFVVAFGQILPSHILNSAKLYPL
                     NVHFSLLPRYRGATPVNTAILNGDDETGTSIMIMDEGLDTGDVIFSAHCNIEPDDNAS
                     RLFEKLVGISIEILDNNWNSICSGNVKRTPQQGVAVKTKLIKKDDLSIDFGENALKIH
                     NKIRAFNYSPGTRTMFRNNILMIEKAEYIKDCEGKPGEITDVEKESFSICCKTGGIKI
                     LELKPAGKRSLKASEFINGYKPQKGEMLG"
     gene            complement(11520..12110)
                     /gene="def"
                     /locus_tag="PL056_00760"
     CDS             complement(11520..12110)
                     /gene="def"
                     /locus_tag="PL056_00760"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HPM45574.1"
                     /translation="MTLLHINTAGDQVLRKKTEPVKEINESIRTLINDMAETMKTASG
                     LGLAAPQIGVSLRIFIIDKGYIDFSSLPDEEKKNKEEPFFPVAFINPQIIEKKGQTSI
                     SEGCLSVPGYHAEVERAAEITLKYTDIEGVERTLEASGLTAIAIQHEYDHLEGVLFID
                     RISNLKKSIAIKKVRKYLQNIKNNGDEIENALYGKP"
     gene            12267..13367
                     /locus_tag="PL056_00765"
     CDS             12267..13367
                     /locus_tag="PL056_00765"
                     /inference="COORDINATES: protein motif:HMM:NF017368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="immune inhibitor A"
                     /protein_id="HPM45575.1"
                     /translation="MKKFLLINIFAVAMIAGLISCGSEEPPEQTETPCKTTADCIRSQ
                     YCDLENPKEDLNTGTLSYFCKDRVQCLKESDCPMNWRCLIEEGFCITDKEAEGVLCKS
                     DADCMTFPNVKCNLATGQCYDPDAGNTGDTGNTGDTGNTGDTGDTGDTGDTGNTGNTG
                     DTGNSGDSGNTGNTGDSGNTGDTGDTGNSGTILVSEDFEDGGTNWTVAGVWQIGAPTN
                     GPLAAVSGSNVAATNLTGNYTDSANDVLMLNTPITIPASGTHVVEFMAWVNIEGGGYS
                     PFDYAEVLVKRDTDTWQAISGIYLTASTPSPLDALDNTRTKITKKLGISYYKFSGDLS
                     SYKGKTVQIGFRFKSDPSDNLEGIYIDNVVVK"
     gene            13461..17213
                     /locus_tag="PL056_00770"
     CDS             13461..17213
                     /locus_tag="PL056_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45576.1"
                     /translation="MKKFFMLSLIFVLAFFVFSCDTKKKKSEDVTDIDGIDADAEITD
                     EEGIIPDDNTDPDEIAVVDDEPDTEKEDEDSVVIDPCNPNPCNEPNRSVCEPDGLGYK
                     CLCDKFTCEINGECFKDGDLNPFDSCSSCNRDFSETEFTVRPDGSECEAVAGTMGSGI
                     CRKGLCGGFGNCDSRAYKQGPGYPCNYDSECATGRCYYLFDWAGGMSVVNVCTGTCKE
                     DEDCPGDMLCQYSNEYGYECLPRFTSTVIKPDPLMADYKPCNNDDDCDGGLCLAYGST
                     TFCSKDCERSSGGGKDLLACGTCGECKDNGDELGFKFKYFCTPDGSGKTGGNCQSGMD
                     CSSGACYDNYCTAACGSLLSPCPSGYECMEDVYQEDVETCVDKARLNIVDGFSCAFDY
                     QCVSGECVEFPYGKYCSKHCDTEACSAGDCVQIGDPAAETPEMACAPLFMPGYAEYGE
                     NCTFDWECEKGLECQDDIKMCTKACEKNEDCPDGVCYAYLEDLLLCVPEHQYGTKPDG
                     YLCYFGYECQNECWPDYTLQKYYCTSKCSDDSDCFDIAGCNESYCNHAYPWRSYTYGM
                     CRFDDDCEKNTLCIEGFCTSECTADSNCAGYAAVVPEEGQKTCKPCTNNTECQNVFYD
                     YGQCVTGSDGSRFCAEDCTDDPSLCPEGTRCYGVGSGGICFPLSGLCSQGHTTCSADD
                     VCIVGRLENDWACREDGECKSGICEGGVCQEGTCSDNDDCGCEMIECKSGNCLPKVSA
                     GTKEVEPNDTVAQAQVLSGSGFTVAYFNHSGNVKETDVFKVSVKKDEYLNVRTHPFCG
                     HDADTYLRFLDSTGKLIGEWENDDINPNGWFFSELLGFFASTAQDIFIEVTQSPLTSQ
                     PQNVPYLLEVQMFTPVSNSACENAEVLTPGTYDRTIQKAVNTIESGACSGGYGYGPDL
                     FYKVNVPAGKLLTFRVTPDSAEFDPELSILSECGVVSEKCLAGNAFGYWGEPEELSYL
                     NSTAQAKDYIVILDTPMLPFEYDFVIQIDITDASVPANDLIEGAIPLSGTGSVNGTTV
                     AANNDYAPTAGICDTADLDGLDVVYSIDLKAGEYFHITVAPTFGAAIYTVKSTDLNTC
                     VSGGGAINYETETDELLYLIIDSASPSTYGTFVIDHIISTTGPCEGLCDTAEHRECSD
                     ETTLCMCDTETGLLKPTDCNQFCIDSNGALSGGCKSNDDGKGCVCTHDCTDTVKVTEL
                     CSDGFITNCTCAASDPCSWVGDTYCNSFCADFFPADHFDDTADCTE"
     gene            17330..18013
                     /locus_tag="PL056_00775"
     CDS             17330..18013
                     /locus_tag="PL056_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45577.1"
                     /translation="MRKFLVIVLLLTCFSLSAASKGKITIQMMGTKAISSSVFNAGDF
                     VFFGVDYSKVRLIGDHGHGSGTNMPVEYIRDTYFNQINELILNETKKYDFSKAIGGKS
                     LKYDFSSVFKINKAAGTENMKAPTEVERHDMKIISQMVKQYSTTQKSKSGYGMVLIAD
                     QMNKYQSTAYLFVVFFDISSKTPVFAAYVKGKAGGFGYRNFWAGAVADVAKQMKKSDW
                     EYWEKSIKE"
     gene            18214..18549
                     /locus_tag="PL056_00780"
     CDS             18214..18549
                     /locus_tag="PL056_00780"
                     /inference="COORDINATES: protein motif:HMM:NF016875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HPM45578.1"
                     /translation="MQIYFKNKKLQKICSVDGEAKKVIGKNAEKKLKQRLAELKAADV
                     LSDISHLPPARLHELTGNRSGIFSVDLEHPYRLLFMAANDPVPLKEDGGIDKRSVTEV
                     EIVEIADTH"
     gene            18560..19639
                     /locus_tag="PL056_00785"
     CDS             18560..19639
                     /locus_tag="PL056_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015906653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPM45579.1"
                     /translation="MMKVLKKLSYEPDYAVAPGETLKETIESLNMTQKELATRTGLTE
                     QTIIRIFKGEQPITYETANKLELVTGVPARFWNNLESNYREQLAKIEEKAKLEKEIKW
                     LDTIEYKELVKRKIIENSKDKYLILKQVFTFFGVSSVDAWKNIWKKPEVAAKRSHAFE
                     TNQGSASIWIRIGEIEANKINCEPFNREKFKDALIKIRRLTTENIETSIPKMQKLCSA
                     SGVALVLVPEMKKVPWSGASKWLNSEKALIILNLRGKSEDKFWFSFFHEAGHILNDNK
                     GRLYIADNSNDPEELKANQFAADFLIPAKYNKLIAALNSKKQITDLAHELGISPGIVA
                     GRFHYLTGNYRQFNDLINTFKWKNL"
     gene            19744..20892
                     /locus_tag="PL056_00790"
     CDS             19744..20892
                     /locus_tag="PL056_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HPM45580.1"
                     /translation="MYAKINPALLIWAVKRSGKSIDSLKNKFPEISEWIEGVTAPTLK
                     QLESFANSTYAPIGFFFLPEPPVETLPLPDFRTLAGEHLQNPSPNLLDTIYICQQRQE
                     WYKEYAKMNGMKPLDFIGSVKEFEDIQVVAEKIRNKLGFDVEKRKKLASWESALSHFI
                     EKVENSGILVMRSGVYMNNNYRHLDVEEFRGFSLSDNLAPLIFINGADTKAAQMFTLA
                     HEVAHIWLGLSGVTDAQLSIVSKSRVERWCNAVAAEILVPVSSIEKEFNPDENLKKEM
                     ARLAKFYKVSTLVILRRIYDLGAISQDKFRIEYRNELERLKSFADSVGSGGGDFYANQ
                     KYKVSSSFAKALLISTLEGSTLHRDAFKLLGFSKFSTFKELGARLGVM"
     gene            20926..22209
                     /locus_tag="PL056_00795"
     CDS             20926..22209
                     /locus_tag="PL056_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45581.1"
                     /translation="MDFENLYKKSQKKVNQKEDYLTELIAFIANISDNFKKIYLKDFL
                     KLKYVRNIDKYSFETQSRKGEETKSIADLVVWKGDEVIAVCEHKIDSDINGDQLTNYR
                     EEYKDCKKFFLVKPEHHKLKIPVGEKKYWGEQIIWEDLFELIKNSVFPEYWKYDNDKD
                     YFKHEKKPKRNSENKLIYYFLDYFVSEGLFCLDMNYYAQEYKSFSNLLKNAKRSVIRY
                     FEYVELLKLEKKSKQGGSKIRDLIYNWRIYIKYKKDYMGFYGYYIDENDSEFCMLDWN
                     KKNKPMDFEKIKKNYFAKFRELNFKSIKHFNTQFEEITDCLQYTNSLLLEIVKSISSE
                     LDMDQSEFKLNAEKETGEMVYDFEYNDISFQISFTDKSLRLYFSGTSKQPKGSIAYEH
                     DEDWRYFEINVENMEFTGKNFSGIKKSFVRYMNTF"
     gene            complement(22236..22625)
                     /locus_tag="PL056_00800"
     CDS             complement(22236..22625)
                     /locus_tag="PL056_00800"
                     /inference="COORDINATES: protein motif:HMM:NF036995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 N-terminal domain-containing protein"
                     /protein_id="HPM45582.1"
                     /translation="MENNLNFDSLLNSIKQADSSFKTNALKAVNVGLTLRNWIIGYHI
                     SEYELNGADRAKYGDNLLINLSKELKTFGVSSTGKRQLYNYHRFYNTYSHIVQTVSTQ
                     FQNLIGVGNADSEKVRTVSTYLIPLAF"
     gene            complement(22658..24073)
                     /gene="amrB"
                     /locus_tag="PL056_00805"
     CDS             complement(22658..24073)
                     /gene="amrB"
                     /locus_tag="PL056_00805"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HPM45583.1"
                     /translation="MWEKKLVRKPAVAGMWYPAEKTELLKMMDELDKKACPEFKQSNR
                     KIAGLIAPHAGFIYSGPTAACGFKQLKNQKYQRVILIGTSHKYKADLISVFDGTEAQT
                     PLGNLKIDTGFSKALISSDPAIQFVPQIHEKEHSLEAMLPFIKFYLGEVEIVLVLAST
                     ENEKLLESAGITIANLIKADSLNTLILASTDMSHYHPYDEAVKMDKSTVSLIESGDYQ
                     KLKKMINDKKCELCGHDSLISFFTAMKELKANKGRLVKYENSGDAVPRSKTQGVVGYM
                     SMIFENNEEISMNYYDDKERKYMLELARNSIVYALKNKKEFVPEKPSDPKLNEQRSVF
                     VTLKIDGELRGCIGSMVATEPLYQAVAGMAFSAAFKDPRFSPVTENDLKLIDVEVSVL
                     TPMQKVSSWKELKIGIDGVYIKKGYRSGVFLPQVATETGWDLETFLSNLCTHKAGLPA
                     DCYKDPTTEIYKYQVELFGEE"
     gene            complement(24059..25069)
                     /gene="amrS"
                     /locus_tag="PL056_00810"
                     /pseudo
     CDS             complement(24059..25069)
                     /gene="amrS"
                     /locus_tag="PL056_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004069120.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
BASE COUNT         7507 a         4753 c         5172 g         7762 t
ORIGIN      
        1 atggcttctt ggcgttggct cagaaacttt gaaaaatttt ggtgccgtaa gcattgaatg
       61 tgcccgtgaa atggctgaag gactgtcaaa aagaagtagc gccgatgtca catttgccgt
      121 cacaggaata gcaggacctg acggcggaag tgaagaaaaa ccggttggaa caatatgctt
      181 tgcactctgc cataacggaa caaccatcac cgaattaaga agattcaacg gtgacagaac
      241 aaacatcaga acaagagcct caatcttcat gctcgacatg ataagaagag cagtttattt
      301 gaaataaatt attatatcgg ttaagggcag gtcttaaaaa agggggactt tccggagatg
      361 agttatgaaa attgttttag ataagattaa ttcaaaagca aaaccttgct taagttctga
      421 attaaaacaa cttttgttca attttccgct gactaaataa ttcatatcat atttcttgca
      481 aatttaattt aattgttgca ttatctattg ttgactaaag ctctggagtc gaaaaatggc
      541 agggaatgaa ctgataaaga ttgatgatat taagaacaga ataataacga ttcgcgatac
      601 acaagttatc ttggacaggg atatcgctga attttttgga gtggaaacaa gaacattaaa
      661 gcaggcagtt aaaagaaata ttgagaaatt cccgtcagat ttcatgtttg aactgactaa
      721 agatgagatg gaaatcatgg tatcacaatt tgtgatacca tcatataaat cttttggcgg
      781 tgcaaaacct ctcgcattca cagagcaagg tgcagcaatg ctttcagcag ttttaaaaag
      841 tgaaatagca actaggataa gtatccaaat tatgagagct tttgtgcaaa tgagaaagtt
      901 tgtcagccac aatattaaaa tatttaacca tattgaactg attgaaaaaa agcttttgca
      961 atattcatca gaatccgaca aaaaatttga gatacttttc aaagcccttg aaaacaatga
     1021 gatcccggcc aagcaaggag ttctgtttga aggtcaggtt tttgaagcac acaaatttgt
     1081 ttcggatgtt gtccgttctg ctaaaaaatc catcattctg attgataatt atgttgatga
     1141 taccgttctt acactgtttt caaaacggga acagggagtt acactcaaaa ttttcacaaa
     1201 aaatctttca aaacagcttc agctcgacac tcagaaattc aacgaacagt tcccacccgc
     1261 agaaatcaag gaattcaacc attcccatga ccggttcatg ataattgacg ataaagacct
     1321 ttatcatttt ggtgcatcgt taaaagatct tggcagaaaa tggttcggat tttccaagat
     1381 gaacatcgaa gttacaaaaa tgctattacg gttataactt ctgaatccgg taagcgatct
     1441 cggtgacggc aacatgaagc agcatgttag ccgcttgaat cattagtttt ttcatagact
     1501 tactctctgt tttaagtgaa aaaagatctg aacaacaaaa aaaataaaat cctgaacaat
     1561 atgttactaa acaccgtgct cattaattaa ccggatatgg agagtgttat gaaagtaagg
     1621 tttgcgtggt tgttagtctt tgtatttact acattttttg tttcatgtga gaacagcact
     1681 aattcaaaga acgatgaaac agttaatgat gaagctgtca cggatgacat gtccgatgat
     1741 ttatccgacg aagttttaac ggagtcggac gaaacaaatg atgaagaatc tgctgaaatt
     1801 cagcttaagt acaaggaatc agatgaaaaa gctgaagcgg ttgaaaatga tctggtaaat
     1861 tccaatacag aatttgcact caaaatgttc aaagcacttc ttgaggcgga taaagataaa
     1921 aatgttttca tttcaccggt ttcaatttca gttgcacttg caatgacgat gaacggagcg
     1981 gcggatgaaa catttgacgg aatgatggag gcacttgaat ttgcaggact tactcttgaa
     2041 aatgttaatg aaagctttct cttgcttctg gaatcacttg aatattcaga taaagatgtt
     2101 cttcttacac ttgcaaattc gatctggatt gcgcctgctt ataagccgga aataagtgaa
     2161 tcattcattg aatcagttga aaaatattat ctgagcggtg tttttgaggc aacttcgcct
     2221 gaagttatca acaaatggat cgaagaccat acaggcggcc acataaagga tatgatcaag
     2281 gaatttcccg atcttcttgt aatgtatctg gtcaatgcga tctatttcaa aggggcgtgg
     2341 acaacaactt ttgacaaaga tgacacctat gaaaccgatt ttacaaaaga agatgattca
     2401 actgtaaaag tgaacatgat ggcttttaag gatatggcgg aatatgaaag ctatgtgtct
     2461 gaaaaagttg taggaatccg gcttccttac ggcagagaga agatctcttt ttatgctttc
     2521 gctcccattg actgggatta tcagccgata gatgaattca taaattctct tgatcaggaa
     2581 tcgtttgatg aaattatcgg gtcttttgat aagcacgagc ttggcggagt tcaccttccg
     2641 aaatttaagc ttgaatatga aaaggaactc aacgaaattc tcaaagcttt cggaatggat
     2701 aaagcttttt caggcggatt tgacaacatg gtaaaagttg acagctctgc caacccttat
     2761 atttcacttg taaaacataa atcattcata gaagtttcgg aagaggggac agaagcggcg
     2821 gccgccacga tagttgaaat tgttgatgaa tcaatgccga caactttcat ggcgaaccgc
     2881 cctttctttt ttgtaatccg tgacgatagg aacggaacga tactcttcat gggtaaagtt
     2941 gcagatcctt cttaagcaga aatctttaaa taattgatat ttttctaaaa taaagtcaca
     3001 atcctccagt tttttttagg aattgaccat gctttatcta atgcttgcag tcatctcttc
     3061 aagttcgatt gcgctgatac ttaaattcag tgaatcaagg gacttgaacc ggctggtcgt
     3121 caccacttca aactatgttg tcgcttcagt tgtgagcttg ttctttgtcc ttgaaggggg
     3181 gcttttcaac agatcagcga ttgatgttcc aacattcttt ttggaaatga atgttatttt
     3241 aaacggtgga atattttcag aaaattcaag ttttatatgg gcgatagcta ccggaatttt
     3301 tggcggaata ctttatttct taggattcat atacattcag aaaagcattc gagataacgg
     3361 ggtcggaatt accggtgcat tttcaaagat agggattttc attccgatga tcatgtcaat
     3421 aattttatgg aaggaatatc cttcaaacat tcagtggatt ggaatagttt tagcagtttc
     3481 tgcaataatt ctggctaatt acacgcctga agtcaaaaat tccctgttta atttcagaaa
     3541 gtcgctgata cttgtttttc ttatcgccgg actcgctgaa ttttcaaaca aatttttcca
     3601 gaactattca atccctgaat ataaatcgct ctttcttctc gttgtttttt caaccgctct
     3661 tatcatcagt tcagttgcaa ccttcattga aagaaaaccg ttcacaaaaa aggatctgct
     3721 tacaggtttc ctcgtcggaa tcccgaacat gctgacatcg ttctttctta tctcttcttt
     3781 caagtatgtg aaagcgacag tcgcattccc cgcatacagt gccggcacaa tagttgtaat
     3841 aaacttaggt ggttatctca tttttaaaga aaagctgatg aaaaaagata tgtttgctac
     3901 agccattata gtttctgcaa tagttttgat gagtttgtga tgtttaaata tcgctatctg
     3961 tgtcatcatc ggaagtttct tccatgacaa cttttacatc ttcgttttta cagtcgtatt
     4021 tgtagattct gccatcgttt tcgtcaaaga tacactccat ttcaatgaaa acatctttat
     4081 ttgaatacct gccggaagaa tcatggaact tcagggaaag attatcatta atatcaaagg
     4141 tgctgttttc tttagtgaat tcgaattctc ctccagaatc agaaactgaa accagatcgc
     4201 actcttcttc cgcataatat ttttcacaaa aatggacatc tatgttttca agaggcaatg
     4261 attcttcgtt tgtcacagtt cctgaaactt taataaacac acagtattct attccataaa
     4321 tcgtatcatc aaagtattct gtgttgcctg tgttgccagt atcgctagtg tttccgctgt
     4381 cctcatcgtt tgagtcagat ttggctattt ctgaatcggt ataattatct gtttttgtat
     4441 ttccacacgc actgaatcct gctaacagtg caaaaacaat tgataaaaac gagaataatt
     4501 ttttcattct ttctctcctt ttagtatcag atctttataa tgacaaagtg aaagtgtttt
     4561 ttcttcatag ttccaaagat gcatcggtcc gccttcacac attttccact ctgagcagtc
     4621 agaacatttg tcggtttttt tccagcttct gtctatgaac ggtttaaatt tattttccca
     4681 gacatcaacc agagagtcgt tgtaaatatt tccctgagat aattttctgt caatgttcgg
     4741 acaggcgagg atagagccgt caaccataac tcccgcaaca tttattccag ccatgcagaa
     4801 aaaaggtctt gaacgaatat cattttcgta aaaaggtccc atatatccgt tgcaggcata
     4861 catcagatcg atctcacggc gatttctgag ctctttgatc ttttccataa atttaacaaa
     4921 ttcatcaggt ttaagaaaca gatctgggtt gtcttttgct cttcctatcg gatcaacacc
     4981 ggtcagccgc catcttttca atcccatttt tttcagcatt tcataaatat catcaagttc
     5041 attaagattt ctgcgattta taactgtcac aacatctttt ttatatattt caggtgtttc
     5101 aaggatcatt ttaagagcat ttttagcttt tctgaagctc tttggattgc ctctgaacca
     5161 gttatgattt tcctcaagtc cgtcaatgga aactgttact gaatgcattc tgctttcttt
     5221 tgcttttttt attgtttttt catcccagct cagtccgttt gtcaccattc cccatggaaa
     5281 ctcaaggtct atgatttttt tcccaagatc ccatactcca ggataaattg taggctctcc
     5341 gcctgaaaga acgatcgtga tcttgtggga gtcatatttc tttttaacat caataagtgt
     5401 cttcaatatg acatcggctg gcaagtcggc tgaattatta tctttgttgc agtcacttcc
     5461 gcagtggaca cagcttaaat tacatttctg agtcagttca aaaaagataa aattgagttt
     5521 gcggaccttc ttatcaaaat agaattttaa agggagaaat gttctgttcc taagcgtctg
     5581 catgtgcaat atgtttcaaa ttactggtca ctgtcatcgt ttgtgtcatc tggatgatca
     5641 ctatcaatag attcataagt atctttgtca tcaccagtcc attcgtgcat ttttaattta
     5701 acatcattgt ttttacaatg ctttaattca catttaagcg aaacttctgc tgtctgtgaa
     5761 gcatattctt tatccgggtc aaccaactct aatttaactt ttccgggaat ataatctgcc
     5821 tcctgttcct gttcagtaaa ctcaaatttt cctttagtat cagattttgc cacttgtttg
     5881 cattcaggat aagaaaaagg gcatattttt acattaatat tttcaagtgg atcgttctcc
     5941 tcatttacaa taacgcctga aatattataa taaattgaat acataactcc gtaagctgta
     6001 tctccaannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngtt gaatctaatt
     6121 tacgatattt ttataatttt tacaattatt tccaggaagt tattcaattc cttttataat
     6181 gagccgtttc ttcagggacg aacattgaga tctcgactaa acctgcgact ttcccgtcgt
     6241 tatcatacca aggggcctga tagatgagct tcttttttcc gtttttctcg attgtataga
     6301 cattcttggt gtcgttttcc atcagatcct tcataatatc ccgggacctc tgctgatggc
     6361 agaacatgag atttttaccg ataagtgact ttccaccgtg tttttcattg acaactgagg
     6421 cagcttcgtt catgtcaaga atgttgccct caaggtccat cactgttatt gcggctccga
     6481 atttttctat ccagccagta aacattttcc acctcaaaat attgtgaatt ctattcctgc
     6541 ggttgatcga aatagtctat cttgatggtt tttgaactgg ttattttcca ggctccgtcg
     6601 atctttttaa gctgatggac gatatttatc ctgttatccc tgaattctcc gccgctcact
     6661 tttgaagttg tctgaacaat ctcgacatcg gctttgttat ccgacatttt aagtatacgg
     6721 gtgctttcaa tggtgagttt caagtcataa ttctcaaaaa gttcaaccat catggctctg
     6781 gtgttctcaa tttcaggaga agtcgggtca agtgttccga tgtaagattc aagatcctct
     6841 ttctgaaaaa attcagcatt gtttttaatg agctgttcaa ttgccgcttt ttctttttcc
     6901 ttactggatg aacaggataa tgacataaga acggaaaaaa ttaccataat gataagaatg
     6961 tatctcgaca ttgtttcctc caaaagaaag ttcatccggt ttttacaatt ttttttaaaa
     7021 taaagccaca atttttccta atagattagt tgccaattat ttccggaaag gtaaaatcgt
     7081 tctgcttaaa tatctttttg acggagaaaa ttatgaaaaa gatcatttcg acaagtaatg
     7141 cacctaaagc gatcggaccc tacagtcagg cagttgaagc aaacggactt attttcattt
     7201 ctggacagct cccggtcgat ccggcaacag gaaagatcgt ggatggtgat atttcagctc
     7261 agaccgatat ggtgatgaaa aatatcggag ctattcttga agcggctggc tgcactttcg
     7321 ccaatgtagt gaaatcaaca gttcttcttc atgatattgc cgattttaaa gccatgaacg
     7381 aagtttatgg caggtattat cctgaaaatt cacctgccag agccgcctat caggtcgcaa
     7441 atctgccact gaacgcaaaa attgaaattg aaacgatagc ggcaaagaat taataattct
     7501 ccggagattc aatgaagttc acagagtttg aaaaggaaaa gctcattttg cttaatgagg
     7561 ccgaatatgg tcgcaacagt gcatatttta actggataac cgaagaagag gggactgtcg
     7621 ctcaaaaaga aagggatgcg cttgttaaag agataaaaga tcttggagca caatttgaat
     7681 ttttaggttc aaaagctgat tcacgatatc tttcaaaggg ttgcaggatt tgcggagagg
     7741 gtggctggtc gtgtcttttc atcaacggga tgtgtaacgc aaactgtttt tactgtccga
     7801 gccgtcagga tgatgctgtt atcaagccga caacaaatac acttgaattt gagagtcctc
     7861 aaaaatatgc tgattatgtg agctttttcg gtttcaaggg ggtaagtttc agcggaggag
     7921 agccgttcat aacatttgac agaactctta agtatatgaa agaattaaga aaacaatgca
     7981 gtcccgatat ttacatctgg ctttatacaa acggatcgct tgttaaccag gagcagttga
     8041 aaatgcttaa agatgaaggt ctgaacgaga tcagattcga tataggtgct ttcggatatt
     8101 cacttgataa agctgaaatg gcggcccgtc acattgaaac tgttaccgtt gaaattccgc
     8161 ttgcaccaga ccatttcgaa acagtcagag atcttgttgt acctatgaaa aatgcaggga
     8221 taaagcatct caacctacat cagctcaggg tgactcctta taatgttaac aaaatgatgg
     8281 atcgggatta caagttcgtc cacggaacaa aagttaccgt ttttgaaagt gagataaatg
     8341 tgctgaaagt tatgctccat tctttgaaga acggcggagt gcctgtcaat tactgctcat
     8401 ttctttttaa aagtggtttc cagtcaagcg cagtaagaaa aagatatgca aaactgtgtg
     8461 ttcaggacac tgaatttatt acacaaaacg gctacatccg taatatttcc caaagtggct
     8521 catcaattac tgtgacatac ttcaatcctg ttgtttcagg cactgatttt tgcaggatag
     8581 aaagaaaaaa ggtcgcagtt ttcaattcag agaatggatt ttcgcacaaa gaccttcatg
     8641 atatgtcgaa ctttgaaatt ctcagaggcg gttttgcaga ctatttctga tgttttgaac
     8701 cagttctctt cttttttcaa ttggtgtccc gaagaaatat gatcccatta ctgcaatatc
     8761 ggcgccggct tcataaacct ttgaaacagt ttcgccatta actccgccgt caaccgatat
     8821 ttcaaaatta aagttgtatt tttcgcggat ctctttcact ttgcgtattt tatcagttgc
     8881 gctttcaata tagctctgtc cgccgaaacc gggatttacg ctcatgacga gaaacaggtc
     8941 acacagatct ccgcagtatt taagaaaact aacggaagta tgcggattta ctgaaattcc
     9001 tgctttaact ccgagctttt ttatgaactg gagcgtgcgg tggaggtggg tttcagtttc
     9061 agcgtgaacg gttatcattt gacaaccagc ttcaacaaaa agaggtgcga acagggttgg
     9121 attgctgatc atcaaatgag catcaaaagg gagagttgtc actttccgga tctgtttaac
     9181 tatcatcgga ccgaaagtga tgttcggtac aaagtttccg tccatgatgt ccagatggac
     9241 aaggtcggct ccgctttctt ctatggaacg gatttcagct tcaagtttta aaaaatcggc
     9301 cgaaaggact gaaggtgcga tcttgatcat tttattttcc tcattgcgca tataaaaaaa
     9361 gtatctctgt tatcgtgaat actgctgacg gtgtaatagg ccttctcaac tttgaaattt
     9421 gtatgcttaa gaagaaattt ctgtatctga tcggtcgttt cctcttttgt gaaggtacat
     9481 accgcaaaaa ggagtgtccc gtcctttttc acataatctg cggcagtatt gagtattttc
     9541 cccgcctgta tcgacatttt ttcaggatcg gagtcgttct taagccattt tatttcagga
     9601 tgtcttctta tcgttccgag tgcactgcat ggggcatcga tcagtacttt gtcaaaagaa
     9661 ctattccatt caggttttgg aatgccggca tcgtgaagtt taacctctat gttgcttttt
     9721 ttgtgtctta cagccgcttc ggtaagcaga ttcagacggt gcctgtttat gtcgacaccg
     9781 acaactttac cggtattgcc tgtcagatac gctgcgaaaa gcgtttttcc tccaggtgcg
     9841 gcacaaaggt caagaacagt atcaccttta tttatatcca tgtcacagac ggcaagctgc
     9901 gaagattcgt cctgaatgaa aacaccggcg atgtctttta tcatgtcata atttacagct
     9961 ttgcttgtat atatcgcata tctgcagtct tttgcggcaa ttgcctcagc cccttttgaa
    10021 acaaggtttg caattacctt ttcttttgtc ttttcatcac cttcgactct cagagttatt
    10081 ccaagaggtc tgttaattat ctgaaggtat gtctctaccg gctcttcagg catcagcgct
    10141 accattcttc tgtacagcca ctcgggaact gaataaaatg tctgaaggaa tttatcggga
    10201 tcttttttaa agaacttctc catattttct tttgaaggat caagccgcag aatctccctt
    10261 aaaatgaaat tgacaaaccc tccggtcttt tcaccaccgc atttctttcc gagttcagcc
    10321 gcttcattta caacaagtgc cgcatccctg ttgttcatga atattatctg ataaagagaa
    10381 gtcctgatta ttgaaagaag ctccggatct atccttttta gaggtttttt gaaagctttg
    10441 aagatccaga aatcgagatg tttcatattt ctgacggttc catagaccag ttccgtgatg
    10501 aatcttctct gactgtattc aaaagaggaa agagagtcgt tgagaagctc gtttatgaac
    10561 agagactggt caagaacggt tcttgtaagg gatgaagcag tgtagcgggg gtctttgact
    10621 ctgttgaatg tcatcccagc atttctcctt tctgaggttt gtatccgtta atgaattcag
    10681 atgccttgag cgatcttttc ccggccggtt tgagttcaag tatctttatt ccaccggttt
    10741 tacagcatat tgaaaaactc tctttttcca catcagttat ctctccgggt ttaccttcac
    10801 aatctttaat atattctgct ttttcgatca tcagaatgtt gttcctaaac attgttcttg
    10861 tcccgggaga atagttgaac gccctgatct tgttatggat tttcaaagca ttttcaccaa
    10921 aatcaattga cagatcatct tttttaatga gtttcgtttt taccgcaacg ccctgctgag
    10981 gtgttctttt cacattgccg gagcatatgg aattccagtt gttatccagt atctctatcg
    11041 aaataccgac gagcttttca aaaagccggc tggcattgtc atcaggttct atgttgcaat
    11101 gggccgaaaa gataacatca cctgtatcaa gcccttcatc cattatcatt atcgaggtgc
    11161 ctgtttcatc atctccgttt aatattgcag tgtttacagg tgtggctcca cggtatctgg
    11221 gcagaagaga gaagtggaca ttgagcggat aaagttttgc actgtttaaa atgtgtgacg
    11281 gaaggatctg cccgaatgca acaacaaaaa taatgtcggg tccgaagtcc ttgattatct
    11341 tatatgcttc atcacttttt gcagattctg tataaactgg aattccgagt tcgagagctc
    11401 ttttcttgac atgtgtgggt tcaagtgcct tacccctttt tcttatttta tctttctggg
    11461 tgaatactat catgtctgtg ttttttgaag caaggaagtt cagaaaaggg acggagatct
    11521 cagggcttcc cataaagagc gttttcaatt tcatctccgt tgttttttat attctggagg
    11581 tattttctga cttttttaat agcgatactt tttttaagat tgcttatcct gtcaatgaaa
    11641 agaacccctt caagatggtc atactcatgc tgaatggcaa ttgcagtaag cccggatgcc
    11701 tcaagagttc tttcaacacc ttcaatgtcg gtgtatttga gagttatttc cgccgctctt
    11761 tcaacttccg catggtatcc cggaacgctc aggcaccctt ctgaaattga tgtctgtccc
    11821 tttttttcaa ttatctgagg atttatgaag gcaacgggga aaaagggttc ctctttgttt
    11881 ttcttttctt catcaggaag agatgaaaag tcgatgtaac ccttatcgat gatgaatatt
    11941 ctgagtgaaa ctccgatctg aggtgctgca agtccaagtc cgcttgctgt tttcatcgtt
    12001 tcagccatat cattaataag tgtccggata gattcgttta tctcttttac cggttcggtc
    12061 ttttttctta atacctgatc gcctgccgta tttatatgaa gcaatgtcat ttttcctcct
    12121 tttcaaggaa acggttcaac gcaaattata aagaaaatct aataacttcc aagaaaataa
    12181 ttgcatttaa atgagaaata aattgaaaaa cagagatatt tcaataaaat atcgttgatt
    12241 ttattttact gttttttgag gcggatatga aaaagttcct gcttatcaat atttttgctg
    12301 ttgcgatgat tgccggtctt ataagttgcg ggtctgaaga gccgcccgaa cagacagaga
    12361 cgccgtgcaa aactaccgca gattgtatca ggagtcagta ctgtgatctt gaaaatccga
    12421 aagaagatct gaataccggc acactttcat atttctgcaa ggacagggta cagtgcctca
    12481 aggaatctga ctgtccgatg aactggagat gtctgataga agaaggtttc tgcattaccg
    12541 ataaagaagc ggaaggagtt ctttgcaaat cagatgctga ttgcatgaca ttcccgaatg
    12601 ttaaatgcaa tctcgcaacc ggacagtgtt acgatcccga tgcaggcaat accggcgata
    12661 ccggaaatac tggtgataca ggtaatactg gagacacagg cgacacaggc gacacaggcg
    12721 atacaggtaa taccggtaat acaggtgata caggtaattc aggtgattca ggaaatacag
    12781 gcaacactgg tgattcagga aacacaggtg atacaggtga cacgggaaat tcagggacga
    12841 tacttgtttc agaagatttt gaggacggag gaacgaattg gactgtagcc ggagtgtggc
    12901 agataggtgc cccgacaaac ggacctttag cggcagtcag cggaagcaat gtcgccgcaa
    12961 caaatcttac aggcaattat accgacagtg ccaatgatgt gttgatgctg aacaccccga
    13021 taacgatccc tgcatcagga actcatgttg ttgaattcat ggcgtgggtc aacattgaag
    13081 gtggcggata cagcccgttc gattatgctg aagtccttgt gaaaagagac accgacacct
    13141 ggcaggcaat aagcggaata tatcttaccg caagcactcc ttcaccgctt gacgcacttg
    13201 acaacacaag gacaaagatc acaaagaagc ttggaatttc atattataaa ttctcaggcg
    13261 atctttcgtc atacaaagga aagacagtcc agatcggatt ccgttttaaa agcgatccaa
    13321 gcgataatct tgaaggcatc tacatcgaca atgtcgtcgt gaaataatcc acaaatattt
    13381 ccattagatt tgtaaaattc ttcatttgtg ataaaatgtc gcccgtgtga tctcattatt
    13441 tttggaacat agggggaaat atgaaaaagt tttttatgct ttcattgatt tttgttctgg
    13501 cgttttttgt tttttcgtgt gacaccaaaa agaagaaaag cgaagatgtc accgatattg
    13561 acggaattga tgcagatgcg gaaattactg atgaagaggg cattattccg gatgataaca
    13621 ctgatccgga tgaaattgca gtcgttgatg atgagcccga caccgaaaag gaggacgagg
    13681 attctgttgt tatcgatccc tgtaacccca acccatgcaa tgagccgaac cgttcagttt
    13741 gcgagccgga tggactcggt tataaatgtc tgtgtgataa atttacctgc gagataaacg
    13801 gagagtgttt caaagacggt gatctgaatc cgtttgattc atgttcttcc tgcaacaggg
    13861 atttttcaga aactgaattt actgtaagac ctgacggaag cgaatgcgaa gcagttgccg
    13921 gtacgatggg aagcggaatc tgcagaaaag ggctttgcgg cggtttcgga aattgtgaca
    13981 gcagggcgta taaacagggt ccgggatatc cctgcaacta tgacagtgag tgtgcaacag
    14041 gaagatgtta ttaccttttt gactgggcgg gcggaatgtc agttgttaat gtctgcaccg
    14101 gaacctgcaa ggaagacgag gactgcccgg gagatatgct ctgtcaatac tcaaacgaat
    14161 acggatatga atgcctccct agatttactt caactgtgat aaagcccgat cctttaatgg
    14221 ctgattacaa accctgcaac aatgatgatg actgtgacgg cgggctttgt cttgcctacg
    14281 gttcgacaac tttctgttcg aaggactgtg aaaggtcaag tggtggcgga aaagatcttc
    14341 ttgcctgcgg aacttgcgga gagtgtaagg ataacggtga cgagcttgga ttcaagttca
    14401 aatatttctg tactcctgac ggatcgggca aaacaggagg gaactgtcaa tcgggaatgg
    14461 actgctcaag cggagcatgc tatgacaatt actgtactgc ggcatgcgga tcactccttt
    14521 ctccatgccc atcgggatac gagtgtatgg aagatgttta tcaggaagat gtggaaacct
    14581 gtgttgacaa ggcacggctc aatatcgttg acggattttc atgtgctttt gattaccagt
    14641 gtgtaagcgg tgaatgcgtt gaattccctt atggaaaata ctgttcaaaa cactgtgata
    14701 ctgaagcatg ttctgccgga gactgcgttc agatcggtga tcctgctgct gaaactcctg
    14761 aaatggcatg tgcacctctt ttcatgccgg gttatgctga atacggtgaa aactgcactt
    14821 ttgactggga atgtgaaaaa ggtcttgagt gtcaggatga tataaaaatg tgcacaaaag
    14881 catgtgaaaa gaacgaagat tgtccggacg gcgtatgtta cgcatatctt gaagatcttc
    14941 ttctttgtgt tcctgaacat caatacggaa caaagccgga tggatatctt tgctatttcg
    15001 ggtacgagtg tcagaatgaa tgctggcctg attatacttt gcagaaatat tactgcacat
    15061 caaaatgtag tgatgattcg gattgttttg acattgccgg atgtaatgaa agttactgca
    15121 atcacgcata tccctggaga agctatacct acggaatgtg cagatttgat gatgactgtg
    15181 aaaagaacac tctgtgcatc gaagggttct gtacttctga atgtaccgct gattcaaact
    15241 gtgccggata tgcggcagtt gtgccggaag aagggcagaa gacatgcaaa ccatgcacga
    15301 ataatactga atgtcagaat gtcttctatg attacggtca gtgtgtgaca ggaagtgacg
    15361 gttcaaggtt ctgtgccgag gattgcacag atgatccatc gctctgtccg gaaggaacaa
    15421 gatgttacgg cgtgggttcg ggcggaatct gtttccctct ttcaggcctt tgttcacagg
    15481 gacacactac ttgcagtgct gatgatgtct gtattgtcgg acggctcgaa aatgactggg
    15541 catgcagaga ggacggtgag tgcaagagcg gtatttgcga agggggagta tgtcaggaag
    15601 gaacttgttc tgataatgat gactgcggtt gtgaaatgat tgaatgtaag agcgggaact
    15661 gtctgccgaa agtatcagcg ggaacaaaag aagttgagcc gaacgatacg gttgcgcagg
    15721 cacaggttct gagcggctcg ggcttcaccg ttgcttattt caatcacagc ggaaatgtta
    15781 aagagacgga tgtttttaaa gtttctgtca agaaagatga atatttaaat gtgaggactc
    15841 acccgttctg cggtcacgac gctgatacat atctgagatt ccttgacagc acgggtaaac
    15901 tcatcggtga atgggaaaat gacgatatca atccgaatgg ctggttcttt tcggaacttc
    15961 tgggcttttt tgcttcaact gcacaggata ttttcattga agttacacag tcgccactga
    16021 cctctcagcc gcagaatgtt ccttatctgc ttgaagttca gatgttcact ccggtttcaa
    16081 acagtgcctg tgaaaatgcg gaagtgctga cgccgggtac atacgacaga acgatccaga
    16141 aagctgtaaa tacaattgaa tcaggagcat gttcgggcgg ttacggttat ggtcctgatc
    16201 tgttctacaa agtcaatgtg ccggcaggaa agcttcttac tttcagggtt acacctgatt
    16261 ctgcggagtt tgatcctgaa ctttcgatac tgtctgaatg cggcgtcgtt tcagaaaaat
    16321 gtcttgcagg taacgctttc ggatactggg gcgaaccgga agaactgtca tatctgaaca
    16381 gtactgctca agcgaaagat tacattgtga tacttgatac tccgatgctt ccgtttgaat
    16441 atgattttgt gatccagata gacataactg acgcatcggt tccggcaaat gacttgatcg
    16501 aaggggcgat accgctttcc gggacaggca gtgtgaacgg aacgactgtt gctgcaaata
    16561 atgattatgc gccgactgca ggaatttgtg acacagccga tcttgacgga cttgatgtcg
    16621 tttattcgat agatctcaaa gccggtgaat attttcacat aacggttgca ccaacattcg
    16681 gtgccgcgat atatactgtc aaatccactg atctcaacac ctgtgtaagc ggcggcggag
    16741 caatcaacta tgaaacggaa acagacgaac tgctttacct cattatagac agtgcatctc
    16801 cttcaactta cggaacattt gtgatagatc acattatttc aacgactgga ccgtgtgaag
    16861 ggctttgcga tacggctgaa cacagagagt gcagtgatga aacgactctg tgcatgtgcg
    16921 acacagaaac aggtctctta aaaccgacag actgcaacca gttctgcatt gacagtaacg
    16981 gtgctctttc aggcggatgc aaatcaaatg acgacggcaa gggatgtgtc tgcacccatg
    17041 attgcacaga cacagtgaaa gttacagaac tctgctcaga cggatttatc acaaactgca
    17101 cctgtgccgc atcagatcca tgttcctggg tcggtgacac ttactgcaat tcgttctgtg
    17161 ccgatttctt ccccgcagat cacttcgacg atactgccga ctgcacggaa tagataagtt
    17221 tatttttctg aaaacagcgc aacatgatcc ctgaaattct tgacaacact ttcatttttc
    17281 tttataagcg ttcgaaacgg tattgccaac gttaattcag gagaagatca tgagaaagtt
    17341 tttagtaatt gttctgcttt taacttgttt ttcgctttct gctgcatcaa aaggaaagat
    17401 cacaattcag atgatgggga caaaagctat ctcttcatct gtttttaatg caggtgattt
    17461 tgtgtttttc ggtgttgatt attcaaaagt ccgtcttatc ggggatcacg ggcacggttc
    17521 gggaacgaat atgcctgtcg aatacattag agacacctat ttcaatcaga taaatgaact
    17581 tattcttaac gaaacaaaga aatatgactt ttctaaagcc atcggcggaa aatcattgaa
    17641 atatgatttc tcaagtgttt tcaagatcaa caaagctgcc ggaaccgaaa acatgaaagc
    17701 tcccacagaa gttgaacggc acgacatgaa gataataagt caaatggtta aacaatattc
    17761 aacaactcaa aaatctaaat caggatacgg aatggttctg atcgccgacc agatgaacaa
    17821 atatcagtca actgcttatt tatttgttgt tttctttgat atttcaagca aaactcctgt
    17881 ttttgcagct tatgtcaaag gcaaggcagg cggattcgga tacagaaact tctgggccgg
    17941 agcagttgcc gatgttgcaa aacaaatgaa gaaaagtgac tgggaatact gggaaaaatc
    18001 tataaaggaa tagttttaag gttctttttt catttctccc caaacagctc cacccgctat
    18061 taataaatct tatattcttg actttgattt aattatcatt tacaggtatt aggattattg
    18121 ttcttgtcgt taatgaattt ttcggtttgc ttgaaaatgt aaaacggagt ttttaactaa
    18181 caaactatgt taatcaattt gacaaaataa aaaatgcaga tatactttaa aaataagaag
    18241 ctgcagaaaa tttgttctgt ggatggagag gcaaaaaaag tgattggcaa aaacgcagaa
    18301 aagaaattga aacaaagact tgctgaatta aaagctgctg atgtattaag tgacatatca
    18361 catttgccac ctgctagatt gcacgaactc acaggaaaca gatctggaat tttttcggtt
    18421 gatctggaac atccgtaccg acttcttttt atggcggcaa acgatcctgt tcctttaaaa
    18481 gaagatggcg gaattgataa acgcagtgtc acagaagtcg aaatagttga gattgccgac
    18541 acacattaga ggtgtaaata tgatgaaagt attaaaaaag ttaagttatg agccggatta
    18601 cgcagttgct ccgggggaaa cattgaaaga aacaattgaa tctctcaata tgactcagaa
    18661 agagcttgca acaagaaccg gtttgacaga acagacgatc attagaatat tcaagggcga
    18721 gcagccaatt acttacgaaa ctgcaaataa actggaattg gttacaggtg tgccggcaag
    18781 attctggaat aatcttgagt caaattacag agaacagctt gctaaaattg aagaaaaagc
    18841 aaaattggaa aaagagataa aatggctgga tacaattgaa tataaggagc ttgtaaaaag
    18901 gaaaatcatt gaaaactcaa aagataagta cttaatttta aagcaggtat ttacattttt
    18961 tggcgtgagc agtgtcgatg cctggaaaaa tatctggaaa aaaccggaag ttgcggcaaa
    19021 acgctctcat gcttttgaaa caaatcaagg atctgcttct atctggataa ggattggaga
    19081 aattgaagca aacaagataa actgtgaacc gtttaataga gaaaaattta aggatgcact
    19141 tattaaaatt agaaggctca caacggaaaa cattgaaaca tctattccta aaatgcagaa
    19201 actatgctca gcatccggtg ttgctctggt tcttgttcct gaaatgaaaa aagttccgtg
    19261 gagcggagct tcaaaatggc ttaactctga aaaagcgttg attattctta acttgcgtgg
    19321 aaaaagtgaa gacaaattct ggttttcatt ttttcatgaa gccggacata ttcttaatga
    19381 caacaagggc agactttata ttgccgataa ttcaaatgat cctgaggaat taaaggcaaa
    19441 tcagtttgcc gctgattttc tgatcccagc aaaatataat aaactgattg ctgcacttaa
    19501 ctcaaaaaaa cagataacag acctcgccca tgaacttggg atctctcctg gaatagttgc
    19561 cggaaggttt cattatctaa ctggaaacta cagacaattc aatgacttaa tcaatacttt
    19621 caagtggaaa aatctataaa gaaacagttt cgatattact tcctgacttg caaaattgat
    19681 tttattttct aaaacattaa agttatagat gtgattattg tcagtttaac ggaaatttga
    19741 acaatgtatg ccaaaataaa tccggcactt cttatctggg ctgttaaaag gtcaggtaaa
    19801 tctattgatt ctctgaaaaa caagtttcct gaaatttctg aatggattga aggggttacg
    19861 gctccgacat taaagcagct tgaaagtttt gctaattcaa cttatgcacc gattggattc
    19921 ttttttctgc ctgagccgcc agttgaaaca ttgcccttac ctgatttcag aacacttgcg
    19981 ggcgaacatt tgcaaaatcc gagtccgaac cttcttgaca ctatttatat ctgtcagcag
    20041 agacaggaat ggtataagga atatgcaaaa atgaacggaa tgaagccgct cgattttatt
    20101 ggatctgtca aagaattcga ggacatacag gtcgttgctg aaaaaatccg taataaactc
    20161 ggatttgatg tcgaaaaaag aaaaaaactg gcttcttggg aatcagcttt aagccacttt
    20221 atagaaaaag tggaaaattc cgggattctt gtgatgcgaa gcggtgttta tatgaacaac
    20281 aattatcgac atcttgatgt tgaggaattc agaggttttt cactttctga taacttggca
    20341 ccgcttattt tcataaatgg cgccgataca aaagcggctc aaatgttcac tcttgctcat
    20401 gaagttgctc atatctggct cgggttgtca ggagtgaccg atgctcagct gtcgatagtt
    20461 tccaaaagca gggttgaacg ctggtgtaat gctgttgcgg cagagattct tgtgccggtt
    20521 tcttcaattg agaaggaatt taatcctgat gaaaatttaa aaaaggaaat ggctcggctg
    20581 gcaaaattct ataaagtcag cactctcgta attcttcgta gaatttatga cttgggcgct
    20641 atttctcaag ataagttcag gatcgaatac agaaatgaac tggaacggct taaatcattt
    20701 gccgatagtg tgggtagcgg cggaggtgat ttttacgcca atcagaaata taaagtgagc
    20761 agcagctttg caaaagctct tcttataagt actctcgaag gtagcactct tcaccgtgat
    20821 gcattcaaac tccttggatt ttctaagttt tccacattta aagaacttgg tgcaagactt
    20881 ggagtgatgt gatttttact ttaatacttt gcggaggaat acaacatgga ttttgaaaat
    20941 ttgtacaaaa aaagccaaaa aaaggtaaat cagaaagaag attatttgac agaactcatt
    21001 gctttcattg caaatatttc agacaatttc aaaaaaattt atttgaaaga ttttctgaaa
    21061 ctaaagtatg taagaaacat tgataaatac tcttttgaaa ctcagagcag aaaaggtgaa
    21121 gaaacaaaat caatagcgga cttggttgtt tggaaggggg atgaagtcat tgctgtttgt
    21181 gaacataaaa ttgattcaga tattaacggc gatcagctta cgaattaccg ggaagagtac
    21241 aaagattgca aaaaattctt tcttgttaag ccagaacatc acaagttaaa aattccggtt
    21301 ggtgaaaaga aatattgggg agaacaaatc atttgggaag acctgtttga attaattaag
    21361 aattctgttt ttcctgagta ctggaaatat gataatgata aagattattt taagcatgag
    21421 aaaaaaccaa aaagaaactc cgaaaacaaa ctaatctatt attttttgga ttattttgtg
    21481 tctgaaggtc tattctgtct tgatatgaat tattacgctc aagaatacaa gagtttttcc
    21541 aatttattaa aaaatgcaaa acgaagtgtc atcagatatt ttgaatatgt tgagctgctt
    21601 aaattagaga aaaagagtaa acagggtgga agcaaaataa gagatcttat ctataattgg
    21661 cgaatataca ttaaatacaa aaaagactat atgggttttt atggatacta tattgatgaa
    21721 aatgatagtg aattctgcat gttggattgg aataaaaaaa acaagccgat ggattttgaa
    21781 aagatcaaaa agaactattt cgcaaaattt cgggaactga acttcaaatc aataaagcat
    21841 ttcaacactc aatttgaaga aataacagac tgcctgcaat acacaaactc attattgctt
    21901 gaaatagtga agtcaataag ctcagaatta gatatggacc aatcagaatt taaacttaat
    21961 gctgaaaaag aaacaggtga aatggtttat gattttgaat ataacgacat ttcctttcaa
    22021 atatcattca ccgacaaaag tttacggcta tatttttcag gtacatcaaa acaaccaaaa
    22081 ggttcgatag cttatgaaca tgatgaagac tggagatatt ttgaaataaa tgttgaaaat
    22141 atggaattta cagggaagaa tttttcagga ataaagaaaa gcttcgtcag atatatgaat
    22201 actttttaaa ataaattttt ttgttggtgt tttattcaaa atgccagcgg tattagatat
    22261 gtggacactg tccgcacttt ttcagaatca gcattaccaa caccaataag attttgaaat
    22321 tgtgtggaca ctgtctgcac aatgtgagaa taagtgttgt aaaatctatg ataattgtaa
    22381 agttgccttt tcccggtgga actgacacca aaagttttaa gctcttttga gaggttaata
    22441 aggagattgt cgccatattt agctcgatct gcgccattaa gttcatattc tgaaatatga
    22501 taaccaataa tccagtttcg aagtgtgagt ccgacattga cagcttttaa tgcattggtt
    22561 ttaaaagaac tgtcagcctg cttaattgaa tttagtaatg aatcaaaatt aagattgttt
    22621 tccattaaac catctcctct tatataataa aaactcctta ttcttcccca aacagctcca
    22681 cctgatactt atagatttcg gtggttgggt ctttgtagca gtcggcgggg agtcctgctt
    22741 tgtgtgtgca gaggttagaa aggaaagttt caaggtccca gccggtttct gttgcgactt
    22801 ggggtaaaaa tactcctgag cggtatcctt ttttgatgta aacgccgtca attccgattt
    22861 ttaattcttt ccaggatgat actttctgca ttggtgtgag gacggacact tcgacatcta
    22921 tgagtttaag gtcattttct gtgactggag aaaaccgcgg gtctttgaat gcggcactga
    22981 aggccattcc ggcgactgcc tgataaagcg gttccgttgc gaccattgag ccgatacatc
    23041 ccctgagttc tccgtcaatt ttgagtgtga caaacaccga tctctgttcg ttaagtttag
    23101 gatcagacgg tttttcaggg acgaattctt ttttgttctt caaagcataa actattgaat
    23161 ttcttgcaag ttcaagcata tattttcttt ctttatcatc atagtaattc attgatatct
    23221 cctcattatt ttcaaaaatc attgacatat atccgacaac tccctgagtt tttgatcttg
    23281 gaacggcatc accgctattt tcatatttta caagccgtcc cttatttgct ttgagttctt
    23341 tcatggcggt gaaaaaagag attaatgaat cgtgtccgca cagctcacat tttttgtcat
    23401 ttatcatttt tttaagcttt tggtaatcac ctgattctat tagtgaaaca gtgcttttat
    23461 ccatttttac agcttcatca tatgggtgat aatgcgacat atctgtgctt gcaagaatca
    23521 gagtgttcaa tgaatctgct ttgatgaggt ttgcaattgt aattccagcg gattcaagca
    23581 gtttttcatt ttcagttgat gcaagaacca gaacaatttc gacctccccg agatagaact
    23641 ttatgaacgg cagcatcgct tcaagtgaat gttctttttc atggatctgc ggcacaaact
    23701 gaattgccgg gtcacttgat attagcgctt ttgaaaaacc ggtatcaatt ttcaggtttc
    23761 caagaggggt ctgagcttca gttccgtcaa aaacagaaat cagatcggct ttatatttat
    23821 ggctagttcc aatgagaata accctttgat acttctggtt tttaagttgt ttaaacccgc
    23881 aagctgcagt tggcccgctg taaataaacc cggcgtgagg agcaatcaga ccggcaattt
    23941 ttctgttact ttgtttaaat tcaggacacg cttttttgtc aagttcatcc atcatcttta
    24001 aaagttcagt cttttcagca ggataccaca ttccggcaac tgccggtttc cgcacaagtt
    24061 tcttttccca cattccgtat atttcacctc cgcagtaacc acattttccg ttatttatat
    24121 tttccgaaac gatctgaaag ccagatctgt caacaacctt ttttccgcag tgcgggcaaa
    24181 tcgtgtcttc gctgttttca gttctcaaat ttcccgtata aacaaatttc atcccctctt
    24241 ttattgccat atctcttgct ttttcaacag tttcgaccgg agtgggcggc ctgtccttaa
    24301 gtttgtaagc aggaaagaat cttgaaaaat ggagtggcgt ttcagtgccg gtattaactt
    24361 ttacccattt tataagtgca aggatttctt catctttatc atttgccttg gggattatga
    24421 ggtttgtgat ctcaatgaaa acacccattt cttttaaagt ttttattgtt tcaagaaccg
    24481 gttttaaaga agcagtcgta tattccttat aaaatttttc gctaaatccc ttaagatcaa
    24541 tatttgccgc atcaatatat ttggcaagtt ctttcagcgg ctcctgattt atgtaaccgt
    24601 ttgttaccat tacagttttc agtccttttt cttttgcaag tttcgccgtt tcaagcatga
    24661 attcataaaa aatgatcggc tcattataag tgaaagctac cgactgactt ccgctttcaa
    24721 ttgctttttc aacaacttgt gatggagtca ttgcttcaaa cgggacatcg tccggagatg
    24781 cttgagatat tgtccagttc tggcagaaat tacagtggag attgcatccg gcacaaccga
    24841 tcgaaaaggt ttttgacccg tagagaaaat ggaaaagagg tttcttttca ataggatcaa
    24901 cattgatcgc aaccggcttg ccgtaggtca gtgcataaag ctttccgccg agatttttcc
    24961 ttgctttgca aagacctgtc tgcccgtcac caagtttgca gtagtgagga cacaaagtac
    25021 attttacatt ttttccatca agagcttcaa aatattttgc gacagtcatc ccttccatgg
    25081 tttttacctc aactctttca ttctttttca ttgaaacacc aatcagtgcc gcagcgataa
    25141 gtatcaccac caccgcaatt tcaattttaa gcatattcac ccccttatct gaggttaaag
    25201 taactccaaa tgttaccaaa attaaatatt tttacaacca tttgtaaata ataagaaaat
    25261 gaagatttcc agttattttt gactctgaat ctct
//
LOCUS       DAOOYB010000006        24046 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_1921_length_24046_cov_9.372932, whole genome shotgun sequence.
ACCESSION   DAOOYB010000006 DAOOYB010000000
VERSION     DAOOYB010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 24046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24046
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1921_length_24046_cov_9.372932"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2681)
                     /locus_tag="PL056_00815"
     CDS             complement(<1..>2681)
                     /locus_tag="PL056_00815"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPM45584.1"
                     /translation="SGNSGNTGDSGDSGNSGDSGNSGNSGNTGDSGEVEPEEIICETL
                     VPLSEGVCEVETGNAFKLIKGNILASDGTYIGGTVLVSDTGVIFCTGCDCDSEPEAAD
                     ATVVTCPQGVVSPALINAHDHMGWTHKNPKVWNDERFDHRHDWRIPLSGHTDLNDSGG
                     ANADQKSWGELRNVMAGTTSIAGSGGADGLLRNVDQNFANTQGIGGIDVHYETFPLGD
                     GNGTLKTSGCDYPSIDSVNLLKNDCYLPHVSEGINLAARNEFLCLSGQGTGSVDLTEP
                     NSAFIHAVGLIASDGEELASNQTSVIWSPRTNISLYGNTAPVTMFDRQGVMIGLGTDW
                     VPTGSINMLRELSCVDFFNKTYLNSYFSDYKMWKMATWNNAVALRIIDVTGSIRPGLA
                     ADISIFDGNDASNFYRAVIGSDESKVALVLRGGKPLYGDKNIVESLDTTADCDGIDVC
                     GNEKSVCVKSETGKTFAELTAANASSYGLFFCTEPTNEPTCVPQRTRIIDEENVYDGQ
                     ITASDSDGDGIEDGEDNCPHIFNPIRPLDGTSQPDEDGDGVGDACDPCPLVKNSSECQ
                     IPDINDRDGDGIPNVDDNCPFDANENQLDSDEDGMGDVCDLCPATPNYLGADCPYEDT
                     TIYEIMRGEKLLGSFVKVQGIVTAKNGKAFYFQVDPDDHDGTLLQKFSGTYVYVTSSS
                     TMPDPALGDKVEVSGRVGDYFGNTQIEYVADIAIITAGKGVPSPVTVLPAEIKTGGEL
                     ADDYKSVLVQVDDAEVTVVADSYHEFTITGGLRVDDYLYDYTNPAVGTKFIYLRGIPT
                     FSFSNSKLQPRSVEDMPVDMCSGVTCDESWSECNADTGNCDPLIGFCAAKVDCPGTDK
                     VCNTATHLCENGDPCADVTCSDEWMECKPEFGACGAK"
     assembly_gap    2682..2781
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2782..3086)
                     /locus_tag="PL056_00820"
     CDS             complement(<2782..3086)
                     /locus_tag="PL056_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45585.1"
                     /translation="MKKIFLAINLILFGILFTSCDNTDVVKETGWVFEDEETFDWDSG
                     DSGNSGDSGNSGNSGNTGDSGDSGNSGDSGNSGNSGNTGDSGNSGNTGDSGDSGNSG"
     gene            complement(3157..4302)
                     /gene="pgsW"
                     /locus_tag="PL056_00825"
     CDS             complement(3157..4302)
                     /gene="pgsW"
                     /locus_tag="PL056_00825"
                     /inference="COORDINATES: protein motif:HMM:TIGR04332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate system protein"
                     /protein_id="HPM45586.1"
                     /translation="MKKVYWKPVKTSWQIHVIIAIVSLICIASVELFKIKIKKSNYQE
                     KVKAAQLMDSSIKVLKKYRLEKIGPIDPEVDPVDSGIIGTLITPITSNSGVREAKLAT
                     VNPNWAAVMIEIFNKADIQKGDTIAAGFSGSFPALNIAVLSAAEVMKLKVVAISSAAA
                     STWGANIPDMSWLDMERILNENKLISNRSVAASLGGSQDRATGMSKEGKELLRKIIER
                     NGAELIFAKDEQTNLDTRMTIYNEKSGDAPIKAFVNVGGGTISVGTRIGKQLFKPGLN
                     KNPDYEALQIDSVMTRFAKEDVPVIHMTKIRALSGKYGLPYTFSIIPRPGEGALFSSY
                     VYDKVIVSATLFIIVALLIVFIKMGYGGRLFSVDNSKKEKGTSEPMV"
     gene            complement(4302..4757)
                     /gene="pgsC"
                     /locus_tag="PL056_00830"
     CDS             complement(4302..4757)
                     /gene="pgsC"
                     /locus_tag="PL056_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020768050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0070501 - poly-gamma-glutamate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein PgsC"
                     /protein_id="HPM45587.1"
                     /translation="MGQLVTLSIGLGLVISLLFSELLGVAAGGMIVPGYVALHLDSPV
                     TVIFTLAVSWLTWMTVKMISTVMIIYGRRRTVIMLLVGFLYGAFFRSIGYIETVSLMF
                     DLSIIGYLIPGLIAIWIEKQGLIETFSTLITSSVIVRLLLILITGGENL"
     gene            complement(4761..5951)
                     /gene="pgsB"
                     /locus_tag="PL056_00835"
     CDS             complement(4761..5951)
                     /gene="pgsB"
                     /locus_tag="PL056_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0070501 - poly-gamma-glutamate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate synthase PgsB"
                     /protein_id="HPM45588.1"
                     /translation="MTLYILFAVLLGSVAAGIFESRKHQANLKKISVRIHVNGTRGKS
                     SVTRLIAAGLNAGGIKTFAKTTGTVPRVITPDGEEFAVYRPSGANIIEQLRIVNFAAK
                     NGAESLVIECMALNPAYQALCELKLVRSTIGVITNARADHLDVMGPDEISVAKALLGT
                     TPVNGTLFTCEEDYVELFKSAGKDRGTNVEIIGREISADVPSEWMDGFSYIEHKENVA
                     LALAVCEKAGVPAEIAIKGMHKVQPDAGAMNEYVVEWFGRKLIFVNGFAANDPESTEM
                     IWDMALTRNNGVKKKIMVVNCRSDRPERSDQTGYAIVNWEKADHYIAIGTGTYIFIKA
                     AVRAGIPPSRIIDMESEDASRIFEKIVEMSNGATLIMGICNIKGTGMELVKYFSNRSH
                     SDKR"
     gene            6084..6965
                     /locus_tag="PL056_00840"
     CDS             6084..6965
                     /locus_tag="PL056_00840"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HPM45589.1"
                     /translation="MNVETQTYSAFIAARNKRIAEEYSVLLDSLNISNNVIYRLGSWV
                     VEVNVYDHSRAVDEIYSYERSGRIDKVKNRFGHIRFQKVNVFYFISALAAMAVFHFIV
                     YDNPGADLIKLGRSSASAITDGQIWRAVTGLTLHADIKHLFSNIIFGGIAVFSLATLV
                     GTGYAWLLYLISGFAGNMLNAHFYGSAHNSIGASTAVFGTVGVLAGLQFFEKFREKKT
                     YAWIPFGAALGLLAMLGSSENTDFMAHLFGFLSGIPLGIITGRIYSKKDLPGRLAQTL
                     FMVVFWAVFILSWMRAL"
     gene            complement(6979..7620)
                     /locus_tag="PL056_00845"
     CDS             complement(6979..7620)
                     /locus_tag="PL056_00845"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HPM45590.1"
                     /translation="MKELKGLSPSTEKLLLKLSKFELLNDFSFVGGSALAIRLQHRLS
                     EDIDLFTWNRSLDKENVFTTLKMLETPFSILSASDKQINVIAGNVKLTFFAQGWDVLK
                     DRELIRENLYTVPVETLTGMKVNTLFLRAKFRDYYDLFVLNKEVFPIETIFNIGQRIM
                     PELTMKLFQTSLVYTKDIAEDNIHHLSPKYNTSINEIAGHFQGEIEKWLSKCD"
     gene            complement(7586..7900)
                     /locus_tag="PL056_00850"
     CDS             complement(7586..7900)
                     /locus_tag="PL056_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45591.1"
                     /translation="MNKSDFIKQLETKHIFWSYSTKTDIPDEIVIEHTLKFGDVVDII
                     NLFKIYREGEISRVWLETMSNDERFEKTNFYLLMFFLKNIEKNKPFETRYERIKRAVT
                     VN"
     gene            complement(7975..8799)
                     /locus_tag="PL056_00855"
     CDS             complement(7975..8799)
                     /locus_tag="PL056_00855"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HPM45592.1"
                     /translation="MRKINNILVYFRKDGKRVDEQRSLMKLFLKYADLYGVSVVDAGE
                     EDKKADLVISGGGDGTFLDAARIAFLYDVPVAGINIGHMGFLTEINPDESLMIEKLFK
                     GEFDVNTRMMIDVAVARGGDDIFNYTALNESIIHRETSSGMICFGIDYNDEKLPEYRG
                     DGVIISTPTGSTAYNLSCSGPIIYPTENSFVINAIAPHALTHRPIVLPADGTIKVHLK
                     DCGKAALLCDGKSSVDIFENDEIAIKKSSRKLQVVSNPDRSFFDILSNKLHFGRRM"
     gene            complement(8786..9565)
                     /locus_tag="PL056_00860"
     CDS             complement(8786..9565)
                     /locus_tag="PL056_00860"
                     /inference="COORDINATES: protein motif:HMM:NF013589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HPM45593.1"
                     /translation="MTHPDLSVYRIKDRGLVISVCSKIDIMSHYLDFYIENGVEDVSK
                     EGFEEMKKLLAFCCAFTRFLPRDTIFSCNVSLPISKRKHFCVFDVGSKTFVARSHKWM
                     PKEYDKSPRLAVQKASVSSDINSVSMVDCLDEPENDMIVNMLIDRYFEVHGGGLFRVF
                     EGNSGFFVVSFLESDEESREKLLDLIPADLEKTVELSEKADKYFYEFKCGCDAQRMSS
                     LFSVISKEDIDYLFENGDEISVDCPRCGLKYTFKRGDLEKN"
     gene            complement(9571..10956)
                     /locus_tag="PL056_00865"
     CDS             complement(9571..10956)
                     /locus_tag="PL056_00865"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HPM45594.1"
                     /translation="MLEIFDNYQLVSKIATGGMAEIFLAKHINSPVNSMPIAIKKVLK
                     QYSSNNALIKMFLAEARIICNINHENIVRIYDFGKYEEQYFIAMEYVFGQNLGSILNK
                     LAQKGSKMPLNLVIEILLGVLSGLDHAHNTKDKNGIFLNVVHLDMNPNNILISYDGKI
                     KVVDFGIANANYTKKAKGSLVSIQGTYGYLSPEQCREGQVDRRSDIFSVGIILYEMLT
                     GQPLYKHLENDATILNAILNEDIPHIKEVVPEIDPRLADIVMKALEKNQKNRYATALD
                     MREDLLAVYNSLEFDPNTESLQSLLKKMFPSHFIKMTKVIERAQTEYLMEELFKDIGE
                     LEELDMKEKVRVQKQEEDELRKKEGILPSTGKIFAAGLVFALILAAVIGYLFITPDIE
                     FEAVSIFSQPPEAEIYLNGEDTGKQTPATLMLEQGKNYIIEFRKDDLIGGMEFTPTEK
                     NREVNIVLRKR"
     gene            complement(10962..12584)
                     /locus_tag="PL056_00870"
     CDS             complement(10962..12584)
                     /locus_tag="PL056_00870"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPM45595.1"
                     /translation="MVKVVVTFSPKGGAGTSVITANLAIYLAQKGKKVLLMDAALNGG
                     TLHTYLNLPSYAVSEDVSEHFSVLPLITSDYQNLSFFSNLKGAVGNLKVSDHLVRWQT
                     ELRQSQFDFLFIDMGSRIDHDLLDTIGMVDYSIMFMSSDHVSIEKSNYFFKELVNYRF
                     RNLELKYDLSYTVQNLKRTRKDMLFTFRNLLLMTSSVAPKNAAQVAEVINGLKIGIVY
                     NAIRSSSEKELASLYQFIIRSSFGIEADFIGELSYSDVVTTSIATMKPVVTLEKNGEF
                     VDALDDIASNMSQQLFQKKGVDQKKKNRLSPFTYYEMLGLDRGCSTLDINNQYEKLKK
                     VLNIDNPVLRAVFDDEQLYVFNSLLESVYKQLSDHEIRKEYDMEIEQHLNSVEDSFPD
                     IFLLSEIVKKYYRSKKGTTKLVKKDVFGREAERTGENVEELDFVDVNKIFEKFRNENI
                     IGSVLKKIREESGISVKSIVTSTKISHFIITAIENDNYSQLHADIYVRGFLKNYCRAI
                     KLNRENTEKVVSDFLKAKNRLMKNDSDPKIEG"
     gene            complement(12656..13327)
                     /locus_tag="PL056_00875"
     CDS             complement(12656..13327)
                     /locus_tag="PL056_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45596.1"
                     /translation="MKIVNISSVFLISCLCFVFFCSCNSKMAAVKNADQMECKKVDLE
                     ESWLFDWNKTRECSVKMKRRGLDEATAQKMDFIEGEWYYLKNDPDGAVYKFASAVKGP
                     DCLVSKASVYYLKRMGHRLNEGDLLSIEECKQDPGSDFQRYLALLGQKSEDPRSVDLD
                     KFHELYSNVLGSDTPPLLYNIALSAIECSNFKQGYELLRNISLRFPEFEKVSAVLKEL
                     DIKDE"
     gene            complement(13314..14408)
                     /gene="mtnA"
                     /locus_tag="PL056_00880"
     CDS             complement(13314..14408)
                     /gene="mtnA"
                     /locus_tag="PL056_00880"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HPM45597.1"
                     /translation="MKRAPEQIVSLKDVPAKTLPSFWKDDKYYVLDQLLLPENIKYIE
                     CSSPENVAETIRNMNLRGAPLIGAAGSAAVALYFNDASIPKDAFNALLKMLLETRPTA
                     VNLKNVLEEIKDTYERNSHLQGKELYNLFRSFSEFVHKRDEERNFKMGENGAVFLESL
                     FNGKKLRILTHCNAGALATCGYGTALGVIRSLNRRNLIEHVWVDETRPYLQGSRLTAF
                     EMVSENIPHTVITDSTAAWLMHEKKIDAVITGADRVAANGDLANKIGTYSLAVNAHHH
                     GIPFLAAMPLETFDRNIPDGHFIVIEERNDEELLVFNGKRIAPELSKGLHLGFDVVPV
                     SLVTALVTEKKVLKKDITQEAISGLFNEDS"
     gene            complement(14374..15342)
                     /locus_tag="PL056_00885"
     CDS             complement(14374..15342)
                     /locus_tag="PL056_00885"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HPM45598.1"
                     /translation="MTLKLIVENDRNELNVHDFDGNYIAAGRSSNNDLVLNERNVSRH
                     HFQLEIVDNRIIIEDRGSYNKTFVNDREISEKMEIFVGDVISIGDYNIYLEQDEDSAL
                     KTASTAKSSKKTALDNLLLAKSGYIGGKTFPLKGAETVIGSHVSADVYLYGPEIPAMH
                     SKLIFDGNMYLLVKGDYKGRYSLVVNDMEIDSVDMRHGDEVKVGDFIFEFIEKGAEYD
                     PVPYQIIAEEARKEKLRKDIKGNKIKSVRDEEITEITQVKKVGSKWSFYVAVGAGALV
                     VIAAIVAAIFVFMRSSKEDPEGLGMKKIYRITIIKNEKSSGTDRVA"
     gene            complement(15373..16440)
                     /locus_tag="PL056_00890"
     CDS             complement(15373..16440)
                     /locus_tag="PL056_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45599.1"
                     /translation="MLVPFERNFKYINKFNAVYIPTFLNDLFKFENISGGLMVCVSNE
                     KVGYILFRDKKIVSANGLYFSTGGELPGNVPFSVICDSSGIDVYVNVIEDAHVIDALS
                     CYLNSAVVFAAPFELSDMKRITGVIEAEKETGILGFKHGAVMNLAEYHKGLLKTFYYY
                     HTGTKSYAFDTNPATFSSYLLSVDKLKPFVIYKRMSAGDVSRPDKNELEFLQNDPLLA
                     MTMAYIDIFELVCKAMKEKLDVPKIAEVFEQMLKVLRDKYSPLYTTISYSPEKGCVNW
                     ADLYNERRYISIEYRFGEYHLYLDELLRLMLRISSSALGSRVNEKLVPGIRKYIEFMD
                     KKDLIMKEMAHRVDKMVEKVK"
     gene            complement(16557..17900)
                     /gene="gdhA"
                     /locus_tag="PL056_00895"
     CDS             complement(16557..17900)
                     /gene="gdhA"
                     /locus_tag="PL056_00895"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HPM45600.1"
                     /translation="MNKYVAAVIKDLKTRYPWETEFLQAAEEILESLGPVMDKEPKYQ
                     ANKILERLVEPERVICFRVPWIDDKGEYHVNRGYRVQFNSAIGPYKGGIRFHPKVTLS
                     TLKFLGFEQIFKNSLTSLPMGGGKGGSDFDPNGRSDNEIMRFCQSFMAELFRHVGPDT
                     DVPAGDIGVGGREVGYMFGMYKKLANEFTGVLTGKGLSFGGSLIRPQATGFGVVYFAE
                     EMLKTKKDSMKGKTVAISGFGNVSWGAVQKCNHLGAKVVTISGPDGYIYDEKGISGDK
                     IDYMTEMLKKDRMSVAPFAKKYKSAKFFPGKRPWEVKVDVALPCAIQNELDLNDAKML
                     IKNGCKQVVEGSNMGCTPEAINLFIQKKIAFAPGKASNAGGVATSGLEMSQNSMRYSW
                     TSEEVDAKLHMIMKNIHKACVEAAKEYGFEGNYVIGANIAGFKKVADAMIAQGLV"
     gene            18086..18667
                     /locus_tag="PL056_00900"
     CDS             18086..18667
                     /locus_tag="PL056_00900"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPM45601.1"
                     /translation="MDRKVSVLSDADDLILLDLAVDKKDQRAWTELVKRYYGKVSATV
                     YRIFGYNSEAEDIVQEVFMELAKSISNFRRDSSFSTFLYRVSVNTAYRYIKRNTSKDV
                     VSDSIEFLSNMLMSKKDDGFTLEKRETAAAVNNALKSLSPEKRMAIVLFKVEGLTLKE
                     ISEVLKVPLQTVWSRIYNGRKELFAKLSDSLNS"
     gene            18677..19888
                     /locus_tag="PL056_00905"
     CDS             18677..19888
                     /locus_tag="PL056_00905"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HPM45602.1"
                     /translation="MEQKLHINIDEVIALFDKNFELLPETVEHIEKCPSCSALIEKYS
                     VTNAVLQSGRHVTSFPDMNRINEIAERSFFILHEGTHPEKEMSRTWHETFRSFFKPLV
                     FTAAAASLLIAIYFGFENPSQNETMTAGTQENDSENKLIPAEKTYEKGLREQGEKIHL
                     SIAKIEALAQTQFNSISENEISMGKGKAKFDVVSGNDFRVTVNGRFMVRVLGTSFILD
                     NCNGTFSVNVIEGLVEVVDRSDDSQFQLSANMEKVFEFKKAVVKNLEISMANKSRIPK
                     TINIPKLNITPDQSFLFQGREALDSGNEGAALQLFMMEMENGKEKDKALFEAVRLHES
                     KKQYNEMVSMIDNNKKILDSSRVYREELLIKGCTAQKKISGSDLSLCRKYLEVFPEGY
                     RKNEIRELINE"
     gene            19881..20663
                     /locus_tag="PL056_00910"
     CDS             19881..20663
                     /locus_tag="PL056_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45603.1"
                     /translation="MNKLFLFLTALMLFVLPSCSTFINLGDSEKSGTNGEDYLPEELS
                     DEEQKESDAGNSGDTGNSGNTGEMSDTGNSGDTGNTGNSGDSGDSGNTGTDDEVSDED
                     SSSLNGYPFDESFTNETCSCGTNPEYDPVCCNGSISVFNACFANCYGIHSSGRICSSY
                     KPGVCGSVIQPVSDDDIVTDEIIDEDIMYPAESVPDEDIAVIDSDSEDFSDDDIFEET
                     PDNECGCFPSDTSFNCCYVNGTVLISSCMAECHCEGGYSNCF"
     gene            complement(20664..21779)
                     /locus_tag="PL056_00915"
     CDS             complement(20664..21779)
                     /locus_tag="PL056_00915"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HPM45604.1"
                     /translation="MQNNITIVVVDDALQCRKHLEKILTDEGYSVILAENGMELEAIL
                     KKNNPSLILLDIIMPGMNGFEVMEQVISANCPMGVPVIFLTGNDDNMSKIKAFDLGAV
                     DYIVKPFNPAEVRARVNVHLRNAIQTNAIIQSQAYKLRQITEARESILVKPEDLPEAA
                     FSVYHSAVLEAGGDFYDVLNTSGKRFVYFLADLSGHDIAASYMVPAIKALIKQNSLPI
                     YSVDETLSMMNRVLLEIMGEEKYLTGFYLVVDRSLGKISWSGAAHPPAVLVPVNGEPE
                     FLKSEGDLIGMFREAKYHSSERKVSKGDRIVIFTDGLLENGESSPVWTHRKDEILKKV
                     SSVSESSLNNMAYELFRKFIPEGTKLQDDVIVLVTEI"
     gene            complement(21769..22359)
                     /locus_tag="PL056_00920"
     CDS             complement(21769..22359)
                     /locus_tag="PL056_00920"
                     /inference="COORDINATES: protein motif:HMM:NF014622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HPM45605.1"
                     /translation="MFRNIYPIVNIKNKQMFNEDFVTRCLKLCPEYLQIRMKDAPVEE
                     ICSVSKKTVWLRNHLNSKTKIIVNDSVDAAVFSGADGVHLGQTDNVLYGLKQKNHGFT
                     VGYSTHTLTEVVKANDLPVDYIGFGPVFKTVTKTQDYTEVFEFVAEVAKISAHPVVFI
                     GGINKRNIESLPSGDRIFYAMVSALDEVLNGVDNAE"
     gene            complement(22352..23086)
                     /locus_tag="PL056_00925"
     CDS             complement(22352..23086)
                     /locus_tag="PL056_00925"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPM45606.1"
                     /translation="MKRIVGQSETAKKMRRFLQYATKKGTYLIGGESGTGKEFLASLM
                     IKKLYENDDRELIVKIKRPEDLSRDGRIYITENRDIFDAVSKVVSSSVWIEPLRERQI
                     DIAELTEYFITEAKVSSDRWYTPKSMKLLVDYWWPYNIRELKRVVTTEDGYKLLPYAN
                     MKKILSNYSATEIVSIKIESFWGELGENVNPGKFYQLFIDSIERAFIKSALKQCGGSV
                     TKTSQLLKIHRNTLAQKMKKFRIKNV"
     gene            complement(23083..23994)
                     /locus_tag="PL056_00930"
     CDS             complement(23083..23994)
                     /locus_tag="PL056_00930"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HPM45607.1"
                     /translation="MDFYLAPLAEITSKEFRALCYEGGADICYSEMISAKAVTLKNRK
                     TLTLSELENGEKKTYLQFFGYDPDTMKQAVEMILSKVTPFGIDINAGCPVRKVIANRS
                     GSALMADPKRLGEIVKAVRSVTDLPLSVKIRKGFKTPNYLECAKVIQDNGADLLIVHP
                     RLQPEMFSGTCDHKVTIELANIMKIPVIHSGDIKKPEDLEKFKGTNISGIMVGRGVLG
                     APWVFDQLKARFVSEERKKEMIEKHFRYYISLGNSKFAHTMIRRHGSWYSTGMNGSPE
                     FRNRIFARSTKIEEAIEMVRDFFSINI"
BASE COUNT         6743 a         5447 c         4360 g         7396 t
ORIGIN      
        1 ttttgctccg catgctccga attcaggttt gcactccatc cattcatcac tgcaggttac
       61 atcggcacat ggatctccgt tctcgcaaag atgtgttgct gtgttacaga ctttatcagt
      121 tcccggacag tcaacttttg ccgcacagaa acctattaac ggatcacagt ttcccgtatc
      181 ggcattacac tctgaccagc tttcgtcaca tgtaacccct gaacacatat caaccggcat
      241 atcttcaaca gatctcggct gaagctttga atttgagaac gagaatgtcg gaattcctct
      301 cagatatatg aattttgtcc ctacagccgg atttgtatag tcataaagat aatcgtcaac
      361 acgaagtccc cctgtaattg tgaattcatg atacgaatcg gcaactactg tcacttccgc
      421 atcgtcaacc tgaacaagaa cagatttata atcatccgcc agttctccgc cggtctttat
      481 ttcagcggga agcactgtta caggggaagg aacgcctttg cccgcagtaa ttattgctat
      541 gtcagcgaca tattcgatct gggtgtttcc aaagtaatcg cccacccttc ctgaaacttc
      601 aaccttatct ccaagtgcag gatcgggcat tgtgcttgaa gatgtgacat atacataagt
      661 tccactgaac ttttgaagaa gtgttccgtc atgatcatca ggatcgacct gaaaatagaa
      721 cgcttttccg tttttagcag taactatgcc ttgaactttg acaaaagaac cgagcagttt
      781 ttcacctctc attatctcat aaattgtcgt atcttcatac gggcagtcag ctccaagata
      841 gttgggcgtt gccggacaga gatcacacac atcacccata ccgtcttcat cagaatccaa
      901 ctggttttca ttggcatcga aaggacaatt gtcgtctaca ttaggaattc catccccgtc
      961 ccgatcgtta atatccggta tctggcattc agaactgttt tttacaaggg gacacggatc
     1021 acaggcatca ccgacaccgt caccgtcttc gtcgggctga cttgtgccgt caagaggtct
     1081 gatcgggttg aatatatgag gacagttgtc ttcaccatct tcgattccgt caccatcact
     1141 gtcagacgca gttatttgac cgtcgtaaac attttcttca tcaataattc ttgttctctg
     1201 aggaacacaa gtgggttcat ttgtcggttc tgtacaaaag aacaaaccat atgatgaagc
     1261 atttgcagca gtaagttcag caaatgtttt tcctgtttcg cttttcacac agacactttt
     1321 ttcattgccg cacacatcaa ttccatcgca atctgctgtt gtgtcaagtg attcaacaat
     1381 attcttatcc ccgtacagcg gcttgccccc tcttaaaacc agagcgactt ttgattcatc
     1441 tgatccgata acggcacgat agaaattcga agcatcatta ccgtcaaata ttgatatatc
     1501 tgccgcgagt cccggtctta tcgagccggt cacatcaatg atcctcagtg cgactgcgtt
     1561 gttccatgtc gccattttcc acattttata atcagaaaaa tagctgttca ggtatgtttt
     1621 gttgaaaaag tctacacatg aaagttccct gagcatattg atggaaccgg tcggaaccca
     1681 gtcagttccc agacctatca ttacaccctg tctgtcaaac attgttacag gcgccgtatt
     1741 tccgtaaaga gaaatattgg ttctcggcga ccatatcaca gatgtctgat ttgaagcgag
     1801 ttcctctccg tccgaagcaa taagtcccac tgcatgaatg aacgctgaat tgggctcggt
     1861 aagatcaaca cttcctgttc cctgacctga taaacacaga aattcatttc ttgcggcaag
     1921 gtttatccct tcagaaacat gaggaagata gcagtcattc ttgagcagat tgactgaatc
     1981 aattgaagga tagtcacaac cgcttgtttt caaagtcccg ttaccgtctc ccagaggaaa
     2041 agtttcataa tggacatcta ttccgccaat tccctgagta ttggcaaagt tctggtctac
     2101 atttctcaac aatccgtctg caccgccgct tcctgcaatt gaagtagttc ccgccattac
     2161 atttctcagt tctccccatg acttctgatc ggcgtttgca ccgcctgaat cattaagatc
     2221 cgtatgtccg ctaagaggaa ttctccagtc atgcctgtgg tcaaatcttt catcgttcca
     2281 tactttagga tttttatgtg tccaacccat gtggtcgtga gcatttatca aagcaggaga
     2341 aacgacacct tgcggacatg ttacaactgt tgcgtcagcc gcttccggtt ctgagtcaca
     2401 atcacatccg gtacagaaaa tcactcccgt atctgaaaca agcaccgttc cgccgatgta
     2461 tgttccgtca ctcgcaagga tattgccttt aatgagtttg aatgcatttc ccgtttcaac
     2521 ttcgcatact ccttctgata acggaacaag tgtttcacaa attatttcct ccggctcaac
     2581 ttcacctgag tcacctgtgt ttcccgaatt tcctgagttg ccggaatcac cactatttcc
     2641 cgagtcaccg gaatcgcctg tgtttcccga atttcctgag tnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn ntcaccacta tttcccgagt caccggaatc gcctgtgttt
     2821 cccgaatttc ctgagtcacc tgtgttcccc gagtttcctg agttgccgga atcaccacta
     2881 tttcccgagt caccggaatc gcctgtgttt cccgaatttc ctgagttgcc ggaatcacca
     2941 ctatttcccg agtcaccgga atcccagtca aaggtctctt catcttcaaa aacccatcct
     3001 gtctctttaa caacatcagt gttgtcgcaa cttgtaaaca aaattccgaa taaaatcaaa
     3061 ttgatagcta aaaaaatctt tttcatgaca aacctcaaaa gaatgaacct gagccattat
     3121 atcttactat gcagcttaaa acaatacaga atttctctag accatcggtt cagatgtccc
     3181 tttttccttt ttagagttat caacgctgaa aaggcgaccg ccataaccca tttttatgaa
     3241 aactattaaa agtgcgacaa ttataaaaag agttgctgaa acaataactt tatcgtagac
     3301 ataagatgaa aacaatgccc cctctcccgg tctgggaatt atcgaaaaag tgtacggcag
     3361 accgtatttt cctgacaatg ctcttatttt ggtcatgtgt atcaccggta catcttcctt
     3421 tgcaaacctt gtcattacac tgtctatctg aagagcttca taatccggat tcttgtttaa
     3481 gcccggtttg aaaagctgtt ttccgattct cgtcccgaca gatatcgttc ctccgcctac
     3541 atttacaaac gcttttatcg gagcatctcc gcttttctca ttatagatag tcattctcgt
     3601 atccagattg gtctgttcat cttttgcaaa gatcagttca gctccattcc tttcaattat
     3661 ctttctcaaa agctctttcc cttctttgct catacctgtt gcccgatcct gactgccgcc
     3721 gagcgaagct gcaacacttc tgtttgatat gagtttgttt tcatttaata ttctttccat
     3781 atcaagccat gacatatcgg gaatgttggc tccccatgta ctcgccgcgg cacttgatat
     3841 ggcaacaact ttcagtttca ttacttcggc ggcagaaagc acagcaatat taagggctgg
     3901 aaatgagccg gaaaagccag cggcgatggt gtcacccttt tgaatatcgg ctttattgaa
     3961 aatctctatc attacagccg cccagttagg gttgactgtt gcaagttttg cctctcttac
     4021 acctgaatta ctggttatcg gagttataag cgttccgatt attccggaat ctaccggatc
     4081 tacttcagga tctatggggc ctattttctc aagacggtat tttttgagca cttttattga
     4141 agaatccatc aactgtgccg ctttaacctt ttcctgataa ttagatttct ttatttttat
     4201 tttgaacagt tcaacggaag caatgcagat aagggaaact atcgcaatga taacatgtat
     4261 ctgccaactt gtttttaccg gcttccaata tactttcttc attataagtt ctccccacct
     4321 gttattaaaa tgagcagcag gcggactatt acgcttgatg ttataagtgt cgagaatgtt
     4381 tcaataagtc cctgtttctc gatccagatc gcaataagcc cggggataag atatccgatt
     4441 attgaaagat cgaacatcag tgaaacagtt tctatatacc cgatacttct gaaaaaagca
     4501 ccgtaaagaa agccgacaag aagcattatc acagttcttc ttcttccgta aatgatcatc
     4561 accgtactta tcatcttaac tgtcatccat gtcagccatg aaactgccag agtaaaaatg
     4621 acagtaaccg gactgtcaag atgaagagcc acatagccgg gaacgatcat ccctcctgcc
     4681 gcaactccca gcaactcgct gaaaagaaga cttatcacca gtccaagacc tatggataaa
     4741 gtcactagct gtcccatttt ttacctctta tcactatgag agcggtttga aaaatatttt
     4801 acaagctcca ttcctgtccc ttttatattg cagatcccca ttatcagcgt tgctccgttt
     4861 gacatctcaa cgatcttttc aaaaatacgg gaagcgtcct cactttccat atcaataatc
     4921 cttgacggag gtataccggc cctgacagcc gcttttataa aaatatatgt ccctgtgccg
     4981 attgcaatat aatgatccgc cttttcccag ttgacgatcg catatcctgt ctgatcagat
     5041 ctttcaggcc tgtctgagcg gcagtttaca accattatct tcttttttac gccgttgttc
     5101 cttgtcagag ccatatccca tatcatttct gtcgattcag gatcgtttgc cgcaaaaccg
     5161 ttcacaaata taagttttct gccgaaccat tccacaacat attcgttcat agctcctgcg
     5221 tcgggttgaa ctttgtgcat cccttttatc gctatttcag ccggaactcc ggctttttca
     5281 cacacagcaa gagccagcgc cacattttcc ttatgctcaa tatacgaaaa accgtccatc
     5341 cactccgaag ggacatcagc cgaaatctct ctgcctatta tttcgacatt tgtacctcta
     5401 tccttacctg ctgatttaaa aagttcgaca taatcctctt cacaagtgaa cagtgtccca
     5461 ttaaccggag ttgttccaag aagtgctttc gcaactgaaa tttcatcagg gcccattaca
     5521 tcaaggtggt cagcccttgc atttgttatc acacctatag ttgatctgac aagcttaagt
     5581 tcacaaagtg cctgatatgc aggatttaaa gccatacatt ctatgacaag cgactccgct
     5641 ccgttctttg ccgcaaagtt aacaattctc aactgctcaa ttatgttggc cccgcttggt
     5701 ctgtaaactg caaattcttc gccgtcaggg gttataactc tcggaacggt tcccgtcgtc
     5761 tttgcaaaag tttttattcc accggcattc agacctgcgg cgatcaggcg ggtcacagat
     5821 gattttcccc ttgttccgtt cacatggatc cttacagata tcttttttag atttgcctga
     5881 tgtttcctgc tctcaaaaat tccagcggca acagatccaa gtagaacggc aaacagaata
     5941 tataaagtca attactgtct cctcatcctg aaatactaaa actaaaaaca gatgaatgat
     6001 attcacttat ttttttttga aaagattttc caaaacccga ttcatcattg attttctctc
     6061 ctcagtgttt ataaaaaccg tccatgaatg ttgaaactca gacatattcg gctttcattg
     6121 ctgcaagaaa caaacggatt gccgaggaat attcggtact tctggattct ctcaatattt
     6181 ccaacaatgt catataccgg ctcggttcat gggtggttga agtaaatgtt tacgatcact
     6241 caagagcggt tgatgagata tattcatatg aaagatccgg aaggatcgat aaggtaaaaa
     6301 accgtttcgg tcacataaga ttccagaagg tcaatgtgtt ttatttcatc tcagctcttg
     6361 ccgctatggc cgtttttcat ttcattgttt atgacaaccc gggagccgat cttatcaagc
     6421 tcggacggtc atctgcttct gccataactg acggtcagat atggagagct gtgacagggc
     6481 tcactcttca tgcggacata aagcatcttt tttccaatat aatttttggc ggtatagctg
     6541 ttttttcact tgcaactctt gtaggaaccg gttatgcgtg gcttttatac cttatttccg
     6601 gatttgccgg aaatatgctg aatgcccatt tttacggaag tgcacacaat tccatcggtg
     6661 cttcaactgc tgttttcgga acggtcggag ttcttgccgg attgcaattc tttgaaaaat
     6721 tcagagaaaa aaagacctat gcgtggatac ctttcggtgc ggcgctcggg cttctcgcca
     6781 tgcttggaag cagtgaaaat acagatttca tggcacacct gttcggattt ctttcaggca
     6841 tccctcttgg aatcataact ggccgtattt attcaaaaaa agatttgcca ggcaggttag
     6901 ctcaaacact ttttatggtc gttttctggg ctgttttcat cctgagctgg atgcgggctt
     6961 tatgatgtat actgtggact aatcacattt tgaaagccat ttctcaattt cgccctgaaa
     7021 atgacctgct atttcattta ttgaggtatt atatttggga gaaagatggt gaatgttgtc
     7081 ttccgctata tcctttgtgt aaaccaatga tgtctgaaag agtttcatcg tcagttccgg
     7141 cataattctc tgcccgatat taaaaattgt ttctattgga aaaacttctt tatttaaaac
     7201 aaaaaggtcg tagtaatctc tgaattttgc acggagaaac agtgtgttaa ctttcatccc
     7261 tgtgagtgtc tcgacaggca cagtataaag attttctcta ataagttcac ggtcttttaa
     7321 aacatcccag ccctgagcaa aaaaagtcag tttaacattt ccggcaatga catttatctg
     7381 tttatcgctt gcgctcagaa tggaaaaagg tgtttctaac attttcagcg tggtaaaaac
     7441 attttcctta tcaagtgatc tgttccatgt gaaaagatca atgtcctcgc ttagccggtg
     7501 ctgaagacgg attgcaagtg ccgacccgcc gacaaaagag aaatcgttaa gcagctcaaa
     7561 ctttgaaagt ttaagaagaa gtttttcagt tgacggtgac agccctttta attctttcat
     7621 atcttgtctc aaacggtttg tttttttcta tatttttaag gaaaaacata agtagataga
     7681 aatttgtttt ttcaaatctt tcatcattac tcatcgtttc cagccatacc cggcttattt
     7741 caccttctct atatatttta aaaagattaa taatatcaac aacatctcca aatttcagag
     7801 tgtgttcaat aactatttcg tccggaatat ccgtttttgt tgaataactc cagaaaatgt
     7861 gttttgtttc aagctgtttt ataaaatcag atttattcat aaaccctccg aaaatcaggt
     7921 aattatagtc caaatcaatt ttatctgcaa ggatttaaat gttttaatat ccggctacat
     7981 ccttcttccg aaatggagct tattcgaaag gatgtcgaag aaacttctgt cgggatttga
     8041 gacaacctga agttttcttg aactcttttt gatcgctatc tcatcatttt caaaaatatc
     8101 cacactgctt tttccgtcgc agagcagcgc cgcttttccg caatctttca aatggacttt
     8161 tatcgttcca tccgccggaa gaactatcgg cctgtgggtc agagcatggg gagcaattgc
     8221 attaatgaca aatgagttct ctgtcggata tatgatagga ccggaacagg aaagattata
     8281 tgccgttgaa ccagtcggag tcgagattat caccccgtca cccctatact caggaagctt
     8341 ttcatcatta taatcaatac cgaaacagat catgcccgag ctcgtctccc tgtgaattat
     8401 cgattcattc aaagctgtat aattaaaaat atcatcacca cctctggcga ccgcgacatc
     8461 tatcatcatt ctcgtattga catcaaattc acctttaaaa agcttttcga tcatcagtga
     8521 ctcatcagga ttgatctcgg taagaaaacc catgtgacct atatttattc cggctaccgg
     8581 aacatcataa aggaatgcaa tgcgggccgc atcaagaaaa gtgccatccc ctccacccga
     8641 aatgacaaga tcagcctttt tatcctcctc tcctgcatct acaactgaaa caccatatag
     8701 atctgcatac ttaagaaata atttcattaa cgatctctgc tcatcgaccc tttttccgtc
     8761 tttgcggaaa tagacaagaa tattgttaat ttttctcaag gtcacctctt ttaaaagtat
     8821 atttcaagcc gcatctgggg caatccactg atatttcatc tccgttttca aaaagatagt
     8881 caatatcctc tttcgatatt actgaaaata atgaactcat tctctgagca tcgcaaccac
     8941 atttgaattc gtaaaaatac ttatcagctt tttcagataa ttcgaccgtt ttttcaaggt
     9001 ctgccggaat aagatcaaga agtttttctc tgctctcttc atcactttct aaaaatgaaa
     9061 caacaaaaaa accggaattt ccttcaaaaa ccctgaaaag accacctcca tgaacttcaa
     9121 aatacctgtc aatgagcata tttacgatca tatcgttttc aggttcgtct aaacagtcca
     9181 ccatgctgac agaatttata tcagatgaca ctgaagcttt ctgtacagca agtctgggac
     9241 tcttgtcata ctccttcggc atccatttgt gagagcgagc cacgaatgtt tttgagccga
     9301 catcgaaaac acagaaatgc tttctttttg agataggaag agagacatta cagctgaaaa
     9361 tggtgtcccg cggaagaaat cttgtgaaag cacaacagaa agccagaagt tttttcatct
     9421 cttcaaaacc ctctttcgaa acatcttcca cgccgttttc aatataaaaa tcaagatagt
     9481 ggctcattat atctatcttt gaacagacag agattacaag cccgcgatct ttaatgcggt
     9541 atactgaaag gtcagggtgg gtcatgaata ttaccgtttt ctgaggacta tgttcacttc
     9601 acggtttttt tcagtcgggg tgaactccat tccccctatg agatcgtctt tcctgaattc
     9661 gattatatag tttttgccct gctccagcat aagtgttgcg ggagtctgct tgccggtatc
     9721 ttcaccattg agatagatct ctgcttcggg cggttgggaa aatattgaaa ctgcttcaaa
     9781 ttcgatatcg ggcgttatga aaagataccc tatcactgcc gcaaggatca aagcgaacac
     9841 cagacctgcc gcaaatatct tgcctgtaga aggaagtatc ccttcctttt tcctcaattc
     9901 atcctcttcc tgcttctgaa cccttacttt ttctttcatg tcgagctctt cgagttcacc
     9961 gatatctttg aaaagttctt ccatgaggta ttcagtctgt gctctttcga tcacctttgt
    10021 cattttaatg aaatgcgacg gaaacatttt cttcaggagt gactgcaatg actcagtatt
    10081 aggatcaaac tcaagtgaat tgtagacagc aagaaggtct tctctcatat caagtgcggt
    10141 tgcatacctg ttcttctggt tcttttcaag cgctttcatt acaatatctg caagccgcgg
    10201 atctatttcc ggcacaactt ctttaatatg gggaatatct tcattaagga tagcgttcag
    10261 gatcgttgca tcgttttcaa gatgcttata aagcggttgt cctgtaagca tttcgtaaag
    10321 aattatgccg acagagaaaa tatcacttct tctatccacc tgcccttctc tgcactgctc
    10381 cggagagaga tatccgtaag taccctggat gctgaccaga cttccctttg ctttttttgt
    10441 gtagtttgca ttggctattc cgaaatcaac gacttttatt tttccgtcat aagatatcag
    10501 aatgttattc gggttcatgt caagatggac aacattcaga aaaataccgt tcttatcttt
    10561 tgtattgtgt gcatgatcaa gaccgctcag aacaccgaga agtatctcta ttacaaggtt
    10621 cagcggcatt ttagaacctt tctgggcgag tttgttcaga attgaaccga ggttctgccc
    10681 gaacacatat tccatagcta tgaaatactg ctcctcatac ttcccgaaat catatatcct
    10741 cactatattt tcatgattga tgttgcatat tatcctggct tcagcaagga acattttaat
    10801 aagtgcgtta ttgctgctgt actgtttcaa aacctttttt attgctatcg gcatcgaatt
    10861 gacaggagaa ttaatatgtt ttgcaagaaa tatttccgcc attccacctg tagctatctt
    10921 tgaaacaagc tgataattat caaatatttc aagcattaaa cttaaccctc aatctttgga
    10981 tctgaatcat ttttcattaa tctgtttttg gctttgagaa aatcactgac tactttttca
    11041 gtattttcgc ggtttaattt tatcgccctg cagtagttct ttaaaaaacc tctgacataa
    11101 atgtccgcat gaagctgtga ataattgtca ttctcaatgg ctgtaatgat aaaatgtgat
    11161 atttttgtgc tcgtgactat tgatttcact gaaatacccg attcttctct aatttttttg
    11221 agaaccgaac ctataatatt ttcatttcta aacttttcaa atatcttatt aacatcaaca
    11281 aaatcaagct cttctacatt ctcacccgtt ctttcagctt cccttccgaa aacatccttc
    11341 ttaacaagtt tggtggtccc tttcttgctt ctgtagtatt tcttaacgat ttcactcaac
    11401 agaaagatat caggaaaaga atcttcaacg gaattgagat gctgctcgat ctccatatca
    11461 tattccttcc ttatttcgtg atcgctcagc tgtttataaa ctgattccag caatgaattg
    11521 aaaacataaa gctgttcatc atcaaagaca gccctgagaa caggattgtc aatattgaga
    11581 acctttttaa gtttttcata ctgattgttt atatcaagcg tactgcatcc cctgtcaaga
    11641 ccgagcattt cataatatgt aaaaggactc agccggtttt tcttcttctg atcgaccccc
    11701 tttttctgga aaagctgctg tgacatattg cttgcaatat catcaagggc atcaacaaat
    11761 tcaccgtttt tctcaagcgt tacaacgggc ttcatagttg ctattgaagt tgttaccaca
    11821 tctgagtaag acaattctcc gatgaaatcc gcttcaatgc cgaaacttga tcttattatg
    11881 aactgataaa gtgaagcaag ctctttttca gaggaacttc ttattgcatt ataaacgata
    11941 cctatcttga gaccgttaat gacttcagcc acctgagcgg cgtttttcgg agcaactgaa
    12001 gaggtcatta aaagaagatt cctgaaagtg aacaacatgt cctttcttgt tcttttcaaa
    12061 ttctgaacag tgtatgaaag atcatattta agttccaggt tcctgaaacg gtaatttacc
    12121 agttccttga aaaagtaatt gctcttctca attgaaacat ggtcagacga catgaacatt
    12181 attgaataat caaccattcc tatggtgtca agaaggtcgt gatcaatcct tgaccccata
    12241 tctataaaaa gaaaatcgaa ctgactctga cggagttccg tctgccacct tacaagatga
    12301 tctgacactt tcaaattgcc gacagcacct ttcaagttac tgaaaaaact aaggttctga
    12361 taatcacttg ttatcagagg gagaacggag aaatgctccg acacatcttc tgaaactgca
    12421 tacgaaggga gattcagata tgtgtgaagt gtccccccgt tgagggcagc gtccattaaa
    12481 aggaccttct tccctttctg tgcaagataa attgcgagat tggcagttat aacagaagtt
    12541 ccggcacccc cttttgggga aaatgtaaca actactttta ccattggttc tccgtcaata
    12601 actcttcaaa ctcaaatatt tataaccctt tttttccagt tctgcaaatt ctttgttatt
    12661 catctttaat atcaagttcc tttaatacag ccgacacttt ttcaaattcc ggaaatctta
    12721 aggaaatatt tctgagaagt tcatatccct gcttaaaatt tgaacactct atcgcagaca
    12781 acgcaatatt atataacaaa ggcggtgtgt cactgccgag tacattgctg tataactcat
    12841 gaaacttatc aagatcaaca gaccggggat cttcactttt ctgtccaaga agtgccagat
    12901 atctctgaaa atcagagccg ggatcctgtt tacactcctc aatgctcagc agatctcctt
    12961 cattcagacg gtgtcccatt cttttcagat agtaaacgga agctttgcta acaagacagt
    13021 cggggccttt cacggcactg gcaaacttat aaaccgctcc gtcaggatca tttttgaggt
    13081 agtaccattc tccttcaata aaatccattt tctgggcagt tgcctcatca agtccccgtc
    13141 ttttcatttt aactgaacat tcccgggttt tattccagtc aaataaccag ctttcctcaa
    13201 gatcgacctt tttacattcc atctgatctg catttttaac ggctgccatt tttgaattac
    13261 aggaacagaa aaaaacaaaa cagagacaac ttatcaggaa aactgaagaa atgttaacta
    13321 tcttcattga acaatcccga aatcgcttct tgcgttatat cctttttgag cactttcttt
    13381 tctgtaacaa gagccgttac aagcgacacg ggaacgacat caaatccgag atgaagccct
    13441 tttgaaagtt ccggtgctat cctttttccg ttaaacacca gcagctcttc atcgtttctc
    13501 tcctctatta caatgaaatg tccgtcagga atgttcctgt cgaaagtctc aaggggcatt
    13561 gccgctaaaa atggaattcc atgatgatgt gcattaacag caagagagta tgttccaatc
    13621 ttgttggcaa gatctccgtt tgccgcgact ctgtcagcac ccgtaataac agcatcgatt
    13681 ttcttttcgt gcatcagcca tgcggccgta ctgtcggtaa tcacagtatg cggaatgttc
    13741 tcggaaacca tttcaaaagc agtaagccgc gaaccttgaa gatacggtct tgtttcatcg
    13801 acccagacat gctctataag attccttctg ttcaacgacc tgatcacacc cagagcagtt
    13861 ccatatccgc atgtagcaag agcgcctgcg ttgcagtgag tgagaatcct cagttttttt
    13921 ccgttaaaaa gagattcgag aaaaactgct ccgttctcgc ccattttaaa atttctctct
    13981 tcatcccttt tatgaacaaa ttccgaaaag ctcctgaaaa gattatacaa ttctttcccc
    14041 tgaaggtgtg agttcctttc ataagtatct tttatttctt caagaacatt tttcaagttc
    14101 actgccgtag gtcttgtttc aagaagcatt tttaaaagag cgttaaaggc atctttgggg
    14161 atcgaagcat cattaaaata aagagcaaca gccgcagagc ccgccgcacc gataagcggg
    14221 gcacccctta aattcatgtt tcttatcgtt tccgcgacat tttccggaga actgcattca
    14281 atgtacttta tattttcagg aagaagcaac tggtccagaa cataatattt atcatccttc
    14341 cagaaagacg gaagtgtttt agcgggaaca tctttaagcg acacgatctg ttccggagct
    14401 cttttcattc tttattattg tgatcctgta tatctttttc ataccgagtc cttcaggatc
    14461 ttctttggat gaacgcatga aaacaaatat tgcggcaact attgcggcaa tgacaactaa
    14521 agcccccgca ccgacagcca catagaaaga ccattttgaa ccgacctttt tgacctgtgt
    14581 gatttctgtg atctcttcat cacggacact tttaattttg ttgcccttaa tgtcttttct
    14641 cagcttttcc tttcttgcct cttcagcaat tatctgataa ggaaccggat catattcagc
    14701 ccctttttca ataaattcaa aaatgaaatc acctactttc acttcatcac catgcctcat
    14761 atcaacagag tcgatctcca tgtcattaac cacaagcgaa tatcttccct tgtaatcacc
    14821 ctttacaaga agatacatat ttccatcaaa gatgagtttt gaatgcattg ccggtatttc
    14881 cggaccgtaa agatagacat cggcactcac atgagatccg attacggttt ccgcgccctt
    14941 caatgggaat gtcttgccgc cgatatatcc gcttttggca aggagaaggt tgtctaaagc
    15001 tgtcttcttt gaactttttg ctgttgaagc agttttaagg gcgctgtctt cgtcctgttc
    15061 aagataaata ttgtagtcac cgatagaaat tacatctccg acaaatatct ccattttctc
    15121 agagatctct ctgtcgttta caaatgtttt gttataactg ccgcggtctt caataattat
    15181 tctgttatca actatttcaa gttgaaagtg gtgccttgag acattccttt cattcaggac
    15241 aagatcattg ttggagcttc ttccggcagc aatatagttg ccgtcaaaat catgaacatt
    15301 taactcgttc ctgtcatttt cgactatcaa ctttaaagtc atcgtatacc tctttcaaca
    15361 aaaattccgc tattatttta ctttttctac cattttgtca actctatggg ccatttcttt
    15421 cattataaga tcttttttat ccataaattc aatatatttt ctgataccgg gaacgagttt
    15481 ttcgttaact ctgctcccca gtgccgacga tgaaattctt aacataagtc tgagaagctc
    15541 atcaagataa agatggtatt caccaaaccg gtactcaatc gaaatatacc gcctctcatt
    15601 gtaaagatcc gcccagttga cacaaccttt ttccggcgaa tagcttattg ttgtatataa
    15661 cggagagtat ttatctcgca aaacctttaa catttgctcg aaaacctcag cgatcttagg
    15721 aacatccagt ttttctttca tcgctttgca aacaagttcg aatatatcta tataagccat
    15781 tgtcatagca agcagagggt cgttctggag aaattcaagt tcatttttat ccggacggga
    15841 aacatctccg gcagacattc ttttataaat aacaaacggt tttaatttat caacagatag
    15901 aagataggat gaaaatgttg ccggattagt gtcaaaagca taggactttg tgccggtgtg
    15961 ataataataa aaagtcttta acagcccttt atgatactca gccagattca ttacagcccc
    16021 atgcttaaat ccgagtatcc cggtttcttt ctccgcctcg atcacgcctg ttatcctttt
    16081 catgtcagac agttcaaaag gagcggcaaa aacaactgcg gaattcaaat aacagcttaa
    16141 tgcatcaatt acatgggcat cctctatcac attaacataa acatcgatac ctgaggagtc
    16201 acatataacc gaaaaaggaa catttccagg aagttcgcca cctgtactga aataaagacc
    16261 attggcggaa acaatctttt tgtccctgaa aaggatataa cccacttttt catttgatac
    16321 acacaccatc aatccaccgg aaatgttctc aaatttaaaa agatcattta gaaaagtcgg
    16381 tatataaact gcgttaaatt tgtttatgta tttaaaattc ctttcaaaag ggaccagcat
    16441 ttttacgctc tacctaaaaa attcaagccg tttataccat ttaagcatat ttttgtaaac
    16501 taaataaaaa aagggggcaa aagcccccct tcgaaaatct attcagaaaa attatcctat
    16561 acaagaccct gtgcgatcat tgcatctgca actttcttaa aaccggcaat gtttgctccg
    16621 ataacatagt tgccttcaaa cccgtattcc ttagccgctt cgacacaagc tttatgaatg
    16681 tttttcataa tcatgtgaag ctttgcgtca acttcttcgc ttgtccatga atatctcatt
    16741 gagttctggc tcatttcaag accgcttgtt gcaactccgc ctgcgtttga agctttgcca
    16801 ggagcgaaag caattttctt ctggataaaa agattgatag cttcaggagt gcagcccata
    16861 tttgaacctt caacaacctg cttacaaccg ttcttaataa gcatttttgc gtcattaaga
    16921 tcaagttcgt tctgaattgc gcatggaaga gcgacatcaa cttttacttc ccacggtctc
    16981 tttccgggaa agaattttgc tgatttatat ttttttgcaa aaggagcaac gctcattctg
    17041 tctttcttaa gcatttcagt catgtaatct atcttgtcac cgctgatacc tttttcatca
    17101 taaatgtatc cgtcaggacc tgatatcgta acaacttttg caccaaggtg gttacatttc
    17161 tgaacggcac cccatgaaac attgccgaaa ccggaaattg caactgtttt gcccttcatt
    17221 gaatctttct ttgtcttaag catttcttca gcaaaataaa caacgccgaa tccagttgct
    17281 tggggacgga tcaatgaacc gccgaaagaa agacctttgc ctgtaagaac gcctgtaaac
    17341 tcgtttgcaa gcttcttata cataccgaac atatagccga cttctcttcc gcctacaccg
    17401 atgtcacctg cgggaacatc tgtatcagga ccgacatgtc tgaaaagttc agccatgaaa
    17461 ctctggcaga aacgcatgat ctcgttatct gatcttccat taggatcaaa atcggaaccg
    17521 ccttttccac cgcccatcgg aagacttgta agagaatttt tgaaaatctg ttcaaaacca
    17581 aggaatttca atgtgctgag tgtcactttc ggatggaatc tgataccgcc cttgtaaggt
    17641 cctattgcgc tgttgaactg tacacgatag ccacggttga catgatattc acccttgtca
    17701 tcgatccacg gaactctgaa acagataact ctttcgggct caacaagtct ttcaaggatc
    17761 ttgtttgcct gatatttcgg ttctttgtcc attaccggac caagagattc aaggatctct
    17821 tctgctgcct gcaaaaactc tgtctcccaa ggataccttg ttttaaggtc cttgataact
    17881 gctgccacat acttgttcat tatcaacctc cataaaaagg aaaaattaga aaaacacctc
    17941 aatgaacacc caacgattgc ttataacaaa ttttaataag gatagtcaag catatttgag
    18001 ttttttcata aaaagatatt ttttttcatt tttttaaata aaatcgacgg ctggatgtta
    18061 cttaacggta atgagagctt ttgctatgga tagaaaagtt tcggtgctca gtgatgctga
    18121 tgaccttatt ctgctggatc ttgcagttga taaaaaggac cagcgggcat ggactgagct
    18181 tgtgaaaaga tattacggca aggtttcggc gactgtctac cggatattcg gttacaacag
    18241 cgaagccgaa gacattgttc aggaggtttt tatggaactg gcgaaatcga tctccaattt
    18301 cagaagagat tcgtcatttt ccacatttct ttacagggtt tcggtaaata ctgcctaccg
    18361 ctacatcaaa agaaacacat ccaaggatgt tgtaagcgat tctattgaat ttctgtcaaa
    18421 tatgctcatg tcaaaaaagg atgacggctt cactcttgaa aaaagagaaa ctgccgcggc
    18481 tgtgaataac gctcttaaat cactctcccc tgaaaaaagg atggctatcg ttctttttaa
    18541 agttgaaggg cttacgctta aagagatctc tgaagtactg aaagttcctc ttcagacggt
    18601 ctggtcaagg atatataacg gaaggaagga actttttgca aaattatcag acagtttaaa
    18661 tagctgaggt accgtcatgg aacagaaact gcacatcaac atcgatgaag tcatcgcact
    18721 cttcgacaaa aactttgagt tgcttcctga aaccgttgag catattgaaa aatgcccatc
    18781 ctgttcagcg cttattgaaa agtattcggt tacgaatgca gttcttcagt caggcagaca
    18841 tgtgacaagc ttccccgata tgaatagaat aaatgagatc gcggaaagat ctttcttcat
    18901 tctccacgag gggacacatc ctgaaaaaga aatgtcgcgg acatggcatg agacattccg
    18961 ctctttcttt aaacctcttg ttttcaccgc ggcagcggca tctcttctca tcgcaattta
    19021 tttcggtttt gaaaatccct ctcaaaatga aacgatgaca gctggcacac aggaaaatga
    19081 cagcgaaaac aaactgatac ccgcagaaaa gacctatgaa aagggtttaa gagagcaggg
    19141 cgaaaaaata cacctctcga tcgcaaaaat agaagctctt gcacagactc agttcaactc
    19201 tatctcagaa aacgagatct caatgggaaa aggaaaggca aagtttgatg tcgtaagcgg
    19261 aaatgatttc agagtaactg taaacggccg gttcatggtg agagtccttg gcacatcatt
    19321 cattcttgac aactgtaacg gaactttttc agtaaatgtc attgagggtc tggttgaagt
    19381 tgttgacaga tctgatgatt cacagtttca gctttccgca aacatggaaa aagtttttga
    19441 gtttaaaaaa gctgtcgtga aaaatcttga gatatcaatg gctaacaaat cccgaatacc
    19501 caaaacaata aacataccaa aactgaatat tactcctgac cagtcattcc ttttccaggg
    19561 aagagaagct cttgactccg gaaatgaagg tgcggcatta caacttttca tgatggaaat
    19621 ggaaaacgga aaggaaaagg acaaggcact ttttgaagca gtcagactgc atgaaagcaa
    19681 aaaacagtac aatgaaatgg tttccatgat agataacaat aaaaagatac tggattcgag
    19741 cagagtttac agagaagagc tcctgataaa aggttgtacg gcacagaaga agatatcggg
    19801 ctctgacctt tccctttgca gaaaatatct tgaggttttc ccggaaggtt acaggaaaaa
    19861 tgagataaga gagcttatca atgaataaac tgtttctttt tctcacagca cttatgctat
    19921 ttgttcttcc ctcctgcagt acatttatta atcttggaga cagtgaaaaa agcggaacca
    19981 atggagaaga ctatcttccc gaagagcttt cagatgaaga acaaaaggaa agtgatgccg
    20041 gcaattcagg agatacgggt aattcaggta ataccggaga aatgagcgat accggcaaca
    20101 gcggtgacac aggaaacacc ggaaacagcg gtgattcagg cgacagcgga aatacgggaa
    20161 cagatgatga agtgagcgat gaagattctt cttcacttaa cggatatcca tttgatgaga
    20221 gtttcacaaa cgaaacctgc tcatgcggta caaaccctga atatgatccg gtttgctgta
    20281 acggcagcat atccgtattt aatgcctgct ttgcaaactg ctacggcatc cattcctcag
    20341 gcagaatatg ttcaagttat aaacccggtg tatgcggatc ggtcatacag ccggtatcag
    20401 acgatgacat tgttactgat gaaattattg atgaagacat aatgtatccg gctgaaagcg
    20461 tccctgatga agatattgct gtgatcgata gtgattcaga agacttcagc gatgatgaca
    20521 tttttgaaga aacacctgac aacgaatgcg gctgtttccc gtcagacaca tctttcaact
    20581 gctgctatgt caacggcact gtactgataa gctcatgcat ggctgaatgt cactgcgaag
    20641 gcggctattc gaactgtttc tgatcagatc tcagtaacaa gaacaattac atcatcctga
    20701 agttttgtcc cttccggaat gaattttctg aacagttcat aggccatatt gtttaaagag
    20761 ctctctgata cagatgaaac ctttttcagg atctcatctt ttctatgcgt ccataccgga
    20821 gagctttccc cgttttcaag aagaccgtcc gtaaatatga ctattctgtc cccttttgat
    20881 acttttcttt cgctggaatg atatttcgcc tccctgaaca ttcctatcag gtcgccttca
    20941 ctctttaaga attccggctc tccgttgaca ggaacaagca ctgccggcgg atgcgctgct
    21001 cctgaccacg atatcttccc aagagatctg tcaacgacca gataaaaacc ggtaagatac
    21061 ttctcctctc ccattatctc aagaagaact ctgttcatca tcgaaagtgt ttcatcaacg
    21121 gaatatatcg gaagtgaatt ctgtttgatg agtgctttaa ttgcaggaac catataactt
    21181 gcggcaatgt catgtccgct aaggtcggca agaaaatata caaatctttt ccctgatgta
    21241 ttcaaaacat cataaaaatc tcctccggct tcaagaaccg ctgaatgata taccgaaaat
    21301 gcggcttcgg gcagatcttc gggttttaca agtatcgatt cccttgcttc cgttatctgt
    21361 cttaatttgt atgcctggct ctgaattatg gcatttgtct gaatggcatt tctcagatga
    21421 acattgaccc tcgctcttac ctcggcagga ttgaaaggtt tgactatata atcaactgct
    21481 ccgagatcaa aagctttaat ttttgacata ttgtcatcat ttcccgtaag aaatattaca
    21541 ggaacaccca tcggacaatt tgccgatatg acctgttcca taacttcaaa accgttcatc
    21601 cccggcatta ttatgtcaag aagaataaga ctcgggttat tttttttcag tatcgcttca
    21661 agttccatcc cgttttcggc aagaattacg gaatatcctt catcagtcag tattttttca
    21721 agatgttttc tgcactgcag tgcgtcgtca acaacaacta tcgttatgtt attctgcatt
    21781 atcaaccccg ttcaacactt catcaagagc ggaaaccatt gcgtaaaaga ttctatcacc
    21841 actcggcaga gattcaatat ttcttttgtt tataccacct ataaaaacaa cagggtgagc
    21901 tgatattttt gcaacttcag caacaaattc aaacacttca gtataatcct gggtttttgt
    21961 aactgtttta aagaccggcc cgaatcctat ataatctacc ggcagatcgt tagctttgac
    22021 aacttcggtc agggtgtgag tcgaatatcc tacagtaaag ccatggtttt tctgcttcaa
    22081 cccgtaaaga acattatccg tctgtccaag atggactcca tcagcccctg aaaaaacagc
    22141 ggcatcaact gaatcattaa ctattatttt agtctttgaa tttagatggt ttctcaacca
    22201 gaccgttttt tttgaaacac tgcatatttc ctcaacaggt gcatctttca ttctgatctg
    22261 cagatattca ggacacagct tcaggcatct ggtcacgaaa tcttcattga acatctgttt
    22321 attcttaatg tttacaatag gatatatgtt tctaaacatt ttttatcctg aactttttca
    22381 ttttctgggc aagggtgttc ctgtggatct taagcagctg agatgtcttt gtgacagagc
    22441 cgccgcactg tttaagggca gactttatga aagccctttc tattgaatcg ataaaaagct
    22501 ggtaaaattt accgggatta acattttcac cgagttcgcc ccagaaactc tcgatcttta
    22561 tcgaaacgat ctctgttgca ctgtagtttg aaagtatttt tttcatattg gcatacggaa
    22621 gaagcttgta accatcttct gtagtaacaa ctcttttcag ttcgcggatg ttgtacggcc
    22681 accagtaatc aacgagcagt ttcatcgatt taggtgtgta ccatctgtct gaagacactt
    22741 ttgcttcggt gatgaaatat tctgtgagct ctgctatatc tatctgtctt tctcttagcg
    22801 gctctatcca aacagaagat gagaccacct tggaaacggc gtcaaaaata tctctgtttt
    22861 cagtaatata gatccgtccg tctctgctga gatcttcagg tctttttatt ttcacgatca
    22921 gttcacggtc gtcgttttca tagagctttt ttatcatcag ggatgcaaga aactcttttc
    22981 ccgtccccga ttcacctccg ataagatatg tccctttttt ggttgcatac tgaagaaacc
    23041 gtctcatttt tttagcagtt tcgctctgtc cgactattct tttcatatat ttatactgaa
    23101 aaaatccctt accatttcaa ttgcctcttc tatctttgtg gatctggcaa agatcctgtt
    23161 cctgaattcg ggacttccgt tcattccggt cgagtaccat gagccgtgcc gtcttatcat
    23221 cgtgtgagcg aacttgctgt tgccgagtga aatgtagtat ctgaaatgtt tttcgatcat
    23281 ctcttttttt ctttcttcgc tgacaaatct ggctttgagc tgatcaaaga cccatggagc
    23341 gcctagaact cctcttccta ccatgatccc tgagatattc gtccctttga atttttcaag
    23401 atcttccggt ttttttatgt cgcctgaatg gattaccgga attttcataa tattcgcaag
    23461 ttcaatcgta actttgtgat cacaagtccc tgaaaacatt tcgggttgca gccgaggatg
    23521 aacgatcaga agatcagctc cgttatcctg gatcactttt gcacactcaa gataattcgg
    23581 agttttaaac ccttttctga tcttcactga aaggggaaga tcagttacag atctcaccgc
    23641 ttttacaatt tcgccaagcc gcttcggatc tgccatcagc gcacttcccg acctgtttgc
    23701 tatcactttt ctaacagggc agccggcatt tatgtcgatt ccgaaagggg tcacttttga
    23761 aaggatcatc tccaccgcct gtttcattgt gtcgggatca tatccgaaga actgcagata
    23821 tgtttttttt tctccgtttt caagctcgga aagagtcaga gttttcctgt ttttaagcgt
    23881 cacagctttt gcacttatca tctcactgta acaaatatca gcaccgccct cataacaaag
    23941 cgccctgaat tcctttgaag ttatttccgc aagtggagca agatagaagt ccaatttatc
    24001 cccgtatcac acaaatcaac aaaactgtaa tatatacttg aaagag
//
LOCUS       DAOOYB010000007        23815 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_1948_length_23815_cov_9.517845, whole genome shotgun sequence.
ACCESSION   DAOOYB010000007 DAOOYB010000000
VERSION     DAOOYB010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 23815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23815
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1948_length_23815_cov_9.517845"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..383)
                     /locus_tag="PL056_00935"
     CDS             complement(114..383)
                     /locus_tag="PL056_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45608.1"
                     /translation="MNKLFNCECCGGGNLNHLRKEIYEHNGGSYNRAISFNGHKRFFK
                     CSECGKIWWTDPISEFMHFVSENAAAGKIIAINNEKSINRALKTG"
     gene            complement(478..2322)
                     /locus_tag="PL056_00940"
     CDS             complement(478..2322)
                     /locus_tag="PL056_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45609.1"
                     /translation="MKKCLVLLMMLSAGFFSCSSKTSTGSDSDNFISDKDPLSENENS
                     DNETDDKSGHVNDDVVLDDDSSSVSDNESDDEDTQDHICDPNPCETEKNIAENRTRCM
                     PADEGRSYTCECDVSYHSSEGLCCQPYSNNVEGECVCNTYYVTPDYDEEQCVPECSEN
                     TIEGLNGYCPEGEICQQGICITDRCYGYECPENSTCSTKNDAPFCQCDTGLQMSDGKC
                     CPLNSTNISGSCECNQGFEFKNDKCEPLASNKCVPNPCEGSGNPLHKNVCVLDGSIEG
                     YHCDCNENYVADEDGCILVEFDFCPEGLQCLSGYCVPFDLSNEQCIEDGDCREFPGAT
                     TVCSSPHAAGGICIGCAVASDCPGNTQCYETYGTCALMCDDDTDCPYGKCYGTGYCGQ
                     KKCYSNEDCFGGTLCIDSDGSGEGMCQRIPCKETLCSKTNPGGSCEDAGASCIAGECI
                     NSCDPNPCKEINRGTCEIKLGVPTCMCDDGTQEINGKCEPVSVTKCPDGFTCASGVCA
                     DKSDSGFVCAESGDCEDADLVCSPVLPSGVCSGCTYPSECPHGGAETSDCVSGYCLKT
                     CFIDTDCNDGMKCLGSGYCGKQDCVKPSDCPSGYTCSSSGRCSRIPCS"
     gene            complement(2322..2690)
                     /locus_tag="PL056_00945"
     CDS             complement(2322..2690)
                     /locus_tag="PL056_00945"
                     /inference="COORDINATES: protein motif:HMM:NF019589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45610.1"
                     /translation="MFRNDLFTHSEFERIEDGFRTTIFFNWKHPVFNGHFPEKPVVPG
                     VLLMQMVKECFETFLSISVVLTGADLKFTNPVTPQTAPELKLEVTFVNDDNKYKVKSN
                     GYDGETKFFKINIELVQGGL"
     gene            complement(2677..3300)
                     /locus_tag="PL056_00950"
     CDS             complement(2677..3300)
                     /locus_tag="PL056_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45611.1"
                     /translation="MLFTSCVTLPCRNCVVEGKTVLNLSQVPFSDTTSALYRVSIKLY
                     SMYFSGLLAVKKLEKDHFRMSLISETGFKLFDMEIKNGIAEMQNVFAELDRSEVKKTF
                     REDFTTMVLSGIEHSDCNGYLFEDSEDFLYECAVSEKLSNNFLIEKKGGLLKKVWKAE
                     DIDEPVFEINYGYNESGSVTPAFITIVHFNVKLRIELSLLEKNNVQK"
     gene            complement(3326..4015)
                     /locus_tag="PL056_00955"
     CDS             complement(3326..4015)
                     /locus_tag="PL056_00955"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HPM45612.1"
                     /translation="MKEILFIALTLFFMLSFAQTVNTENVKSDASAAFTELASSSNVA
                     KWRNTVASNASSIVSIKSSFKQVKTLSFLSQKVTSGGRFFFEKSENGNKLKWEYTTPF
                     FYTIVINGNNITMKDGQKISSFDMSTSRVFQEINEIMVASLNGTILSDKGNFIFDIKE
                     KGRELTISMTPVKGTSLSEYFRNINMIMDKSDFTVSMLTMVEITGDSTELLFIDKKLN
                     GKIDESEFSVK"
     gene            complement(4016..4696)
                     /locus_tag="PL056_00960"
     CDS             complement(4016..4696)
                     /locus_tag="PL056_00960"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPM45613.1"
                     /translation="MVLFSLIIIISIAFIAWASFSISSGIFLKAVCRNRKSERDILLT
                     FDDGPDPVNTPLVLEILDKKGVKALFFINGSKALENPELLKNIFLKGHLIGNHTYGHS
                     NFFPFYSRKRMLFELKKTSDIIENITGKRTLYFRPPFGVTNPSIAYAARELGLITIGW
                     SVRSFDTFLKEKGKILSSVMKKVKGGDIVLFHDTVKGTVEMLDEFIVYCQKEGFELTV
                     PEKFFLED"
     gene            complement(4683..5624)
                     /locus_tag="PL056_00965"
     CDS             complement(4683..5624)
                     /locus_tag="PL056_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45614.1"
                     /translation="MPAYIRSTGILIPHKNEDGTTFRCQKPEIKEFIDPKESRRFSGI
                     IKNGVFAGMTALKNGGIRKPDAIITGTGLGCLSDTIKFISSIKQGGEENLNPAPFIYS
                     THNTVGGTISILTGCTGYNSTFVHRGISFESALFESFLISCEEPELNILCGGVDEMTN
                     EYLVIAERIGLHRENSIPGEGAGFVVLSGKKEGSVAEVSDIKTVPVIDKMQTVKHFRE
                     FIEKNSIDPAKTVIFTPFNGDSECDNWKKDVLKSYKWINIVNPKEVTGEFMTAGALST
                     VMAVDLIIKDPQIKQVLVWNSFLNMENAFIFIKNGSI"
     gene            complement(5626..6834)
                     /locus_tag="PL056_00970"
     CDS             complement(5626..6834)
                     /locus_tag="PL056_00970"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HPM45615.1"
                     /translation="MAKKIYVTAAGIISSIGIGCGQTVKSLVEMKGGQTETRFVDSKL
                     ARNFPLCEVPFSNDELAQIAGVSRNEFKTRCSLLAAVAAKEAAVNAKIRKNDGILTGL
                     VCGTTVGGMDRSELFYREFSNDLQNGGDINDVICHDCGDTAHKVSVLINATDFVTTIS
                     TACSSSANSFITAARLLQHGEVERVVAGGSDALTYFTVNGFNSLRIHDTQPCRPLDAS
                     RKGLNLGEGAAFAVLETEETIKKTGNIPICELAGWSNCAEAYHQTASSPEGNGAYAAM
                     TGALKKAGLNPEDVDYINLHGTGTDNNDASESAAVVRVFKEKMPMLSSTKTLTGHTLG
                     ASGGIEAVISILSIKENMVPGDKMFRIPIEATGLKPLPSTLTDNPVKHIMSNSFGFGG
                     NNSCLIFTRC"
     gene            complement(6836..9727)
                     /locus_tag="PL056_00975"
     CDS             complement(6836..9727)
                     /locus_tag="PL056_00975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPM45616.1"
                     /translation="MKRKIAVALILLFAFILNANLFERMFSTSKDYSISQGEVTIYAL
                     RAVKNKYLNKSKLQAADLLVEGLNAIHDQLPETLIKYDRNRNQVTVQIYNHTYTANVG
                     RMRDLYDIAYVLKQVYGFIEKNYVPDASLEITDVEYIAVNGILKKLDPHSYIFTPKEF
                     EEFTSSTEGNFGGLGIVISVNDDGEIVVVSPMDGTPAMEAGVEANDVIVQIDDESAIN
                     MTLNKAVERMRGKPGTSVTIRIRREGVPDLLKFTITRAEIKIESVVSAMPEPGIGYIK
                     LSGFMENTYPSMLSALEDLKKKGMKSLILDMRNNSGGLLSQAIKISDIFLERGVIVST
                     VGDDEDDVSEAEKQNTDILDIPVTVMINEGTASAAEIVTAALKKNGRASVLGRKSFGK
                     GSVQNLFRIPGGGGMKLTIAQYLTPGGVSIQSVGITPEIEIRPAFIHNEKISLFDMGE
                     NIVTENKLKEHIVSSYIPQKPEKPDIIVRYFKEYKDTDQIVKERRKEKIGAFKSDEEI
                     EIAIKIMKEMISNGIKPNDAAVRIKESEWSGIVSKLTESGIPWKSSMSLRQPDLDSLK
                     VSLLSSQKIIGGSSYQFKFKAEIEGEIENLIGVLETDIPFLRRLEIPFGTFSGSIERT
                     VNIKLPESVPWRKENAQLKLYTGTPDKVIKIKDFVIETMPVDSPDIKLSFLSVDSGGS
                     VDGMIQPGEEVKLLINFTNIGKGAVLDGRAMLINVDNSKEIFINSGTLSFTLAPGETK
                     SAEFKFKLSNFEAKETLATKLAVSLYDYKTKYNSGFSVPIYRSGAGCVFESREAGIVL
                     EKGVEIFSAADLKNVYGIIKERSVLKSTGKCGEAYLSEDGFWIRSENVKTSEEKPSKL
                     QMDIKYSIKMPGIFFDRSPSLSENGKFNIAFEINPAETRDVFVFINDKKYFYKRLSET
                     GSVNKISVPVTLEKKINSISIVAKGYDREKTAFARKHVVFPKGSDNEEE"
     gene            complement(9766..10413)
                     /locus_tag="PL056_00980"
     CDS             complement(9766..10413)
                     /locus_tag="PL056_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45617.1"
                     /translation="MNISMNLSVWTAFAFTLAAFSGLYRDNPVYKFAESVFIGVSAGY
                     FASIWLFSVLIPVFDDTMSGDYILLVPVISGLLLFIPSDGKRKYSKLIFMPSVFIVML
                     YLAASIPVYFQAYVYEMIRSSSIPLISLAENGSIRWDLTINAIVSITGIVSVLLFLVA
                     RHRKVNGFLSAAGETGRFYLLVAIGVSFGYTLVSRIILLMGRFDFVITDLLGFRF"
     gene            complement(10410..11255)
                     /locus_tag="PL056_00985"
     CDS             complement(10410..11255)
                     /locus_tag="PL056_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45618.1"
                     /translation="MRRIGFLLFDIPFKYLAVIPVFIIIIFRFIDTEIDPGMEDNGKK
                     VYEIMENVPAGSKVLIITNFGPEAKYELEETLSAMVGYFAAKDAGIVFATLVPVGVES
                     AFMAVERSIGNPDFIKERYLYGYDYVNIGYIGGGTVGAFLLSSNISGVRKKDIYGNEL
                     KALPIMNGIERFNDFAAVFEFSSRMIDGSPGMTLFSVLSGDENLAKAVICSSDMVPNY
                     LPFHKSGSFEGFAGGFREIAGLTKVIDPSSDIWKRYYIMSMILEYILIIIVLSGIRRL
                     VRGER"
     gene            complement(11252..12553)
                     /locus_tag="PL056_00990"
     CDS             complement(11252..12553)
                     /locus_tag="PL056_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45619.1"
                     /translation="MRRFAVITILIPLVIFSVLVIYADYGRNIFSVILRCGETVIVIS
                     FVAGLLKVLHKEAVKIRKGRFSQWPSAVILLSFLVTFLAGAMNINQSKYAFNNIIREQ
                     PAYLVETADIIIDDDYYLSKFPARKKLSLYVSANVSPYFFPTDEMLKEADMTSEDFKD
                     LKYKKGIFDEKNTVFINEDDSKSVSELEKKVRAMSIEGISKMLPVAASLREWFTDSLR
                     EKGEQDNVQDAAIEGVRIKGSGDMLKEWFDEQNVSGINKNETVKYYADFVRSSGVADI
                     IAESCSKNADMDISKNAASKMFSAFLKTGVTSSPNMKGFVRWIYRSILDPLFSTFIAM
                     LFFSMLLTVYKMLDFRNYSYFVISVSVCLVIAGFFPYFNSVLNFFLSPDWKGPFSEGW
                     LINVFSAPVFKALAIGTGTGVLFYSAETVFEVFTPKNRRGN"
     gene            complement(12550..13602)
                     /locus_tag="PL056_00995"
     CDS             complement(12550..13602)
                     /locus_tag="PL056_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45620.1"
                     /translation="MKKIVFAMLFQSMFYFMILAEDGSSFKIVHNENDKIDIVVNIQD
                     SNVVNVELYRVLRNSPPVNIYEFTFPDNHFSAPSAIRACYFLKIYYDDGTVRETARKC
                     HSEADILKKINISMVFVVFIFIFSILAAGFIKRPFFSSISRDRQSMLKFIVTLLGENT
                     AKISFLSSVDSTGHPLLGPLTALMTGLANSADTGSSIESELFPDGSDSFFQNGRNSFV
                     AVPSFGDLAGFYSAVNRQDWSRQMGFNISDGSAISDWSTYFAGESKRNINLSLNVPGL
                     SGNAILSRKNLISIEPFLLSSQLKNIRSERSVKVSGAYLFLIITAVIITAGSVLSTLA
                     QYFPFITELFNIQAGI"
     gene            complement(13602..15038)
                     /locus_tag="PL056_01000"
     CDS             complement(13602..15038)
                     /locus_tag="PL056_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45621.1"
                     /translation="MKSYHISFIVMQSKIRTVVVEYGDNGTAFFVKDVFEKGDNFNFK
                     EHVRKLMQESSYHKSDQPSKVSSSMMSIPPKLLSAVVTGMTSEGSLHACVKLARSMLL
                     DIKKTFLINEMSESDINSCSEDVLIISGGYDDSENRQLHDFILKLTESQFIKKTKPAV
                     VFAGSSSALPFAKVNIGTLTDFTMLPNVLETSFCPESLVAGEDISIKRSSTLMISSDQ
                     PKVTAFSFSNSLQKVTEMFCGRYVNKVLSVLITEEYSVVNECSRSAGKFHFKRYGLSM
                     DDVSLKTADLSESLRSIFQQESLPDRDFAQEDLLQIEKSERVEYYRPERIIGISLSGK
                     AGFEKFLDVVSDPDLLRGVIEFVFDRDGVLLASAPMFFEKQGDHSGWLLDASYSENMV
                     SGWIVIPDGTFVKERTCLTVYVSGNEERNEMSFLWGKRYVMKIDPFSVIEIDVSEKVY
                     FEGAGRKKVLKTGKAGRTIVFDLRKERS"
     gene            complement(15035..15235)
                     /locus_tag="PL056_01005"
     CDS             complement(15035..15235)
                     /locus_tag="PL056_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45622.1"
                     /translation="MKDQSTALLLLNASRPFKRIILFTLPVFLPLFSIVFGLERGKMI
                     EKIFEDETFFEQLKNSLEKGLK"
     gene            complement(15232..15669)
                     /locus_tag="PL056_01010"
     CDS             complement(15232..15669)
                     /locus_tag="PL056_01010"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HPM45623.1"
                     /translation="MAVDNSKGIAKKVMFEEKERNADEVVFSSRNQRTAQRIDVEIEV
                     TLTGPHTFFSGLTMDISEGGVFVATHQIFPIGTEFRMILFLGKEKLEIDSLVVWVRGE
                     DSAMISGEEPGMGLKFIKLDSRSLAVISEFLKKREPILYDDCL"
     gene            complement(15701..17218)
                     /locus_tag="PL056_01015"
     CDS             complement(15701..17218)
                     /locus_tag="PL056_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015028698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPM45624.1"
                     /translation="MKRHIIIAISFMLLGGCVAGSKLYKSTLLESKRLEVDKNRGADI
                     CAPYDIAMAESHIEFARLELESGNHYRAEEHITAKDLYATKASLNADICIPKDADEDG
                     ILDVEDRCPKDAGTTEFKGCPDKDGDGLPDIDDKCPDVSGPLGLNGCPPAADRDGDGV
                     PDSTDRCPDDKGFAELKGCPDRDGDGLADLDDSCPDIPGVVELKGCPPAKDRDGDGIP
                     DRDDRCPDEKGLKELNGCPPAADRDGDGIPDNTDRCPDDKGTAEFKGCPDRDGDGIAD
                     VDDKCPDAIGPKELQGCPPAADRDGDGIPDSEDRCPDDKGITTLKGCPDRDGDSVADL
                     DDKCPDVPGTVELNGCPQVLDKDGDGINDDVDKCPDVPGVEEEKGCPKKYKLIVVTAK
                     KIELKQVVLFQTGKAVIDRKSYEMLKEVADALKNAPSVKKVIIEGHSDNVGSRNLNIS
                     LSQKRAEAVREFLVDEGVDSGKLEAKGYGPDKPVSSNRTAAGKAKNRRVEFVLEQ"
     gene            complement(17215..>17533)
                     /locus_tag="PL056_01020"
     CDS             complement(17215..>17533)
                     /locus_tag="PL056_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45625.1"
                     /translation="TPAPEVKLEPEPQPEPQPAVTADPEEKPSLKGDPFAEKKEEPKK
                     AEPAKKEEKKPSYYDVYEEMRQKYLKKQSDEKEKEKEEEKKEEQKKEKKKEDSTAPKG
                     GAK"
     assembly_gap    17534..17633
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<17634..18046)
                     /locus_tag="PL056_01025"
     CDS             complement(<17634..18046)
                     /locus_tag="PL056_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45626.1"
                     /translation="MRLVSVVFFAMIPVIFVSCGIARSTSSIWKAEDQREKSLGKIAT
                     AFEKADIVETDEFGRRSLAKKAADEVKPLEKRKAAYYFFKGDAYLSKAYDFRSKSIYD
                     SSDFLATKALEYFNEAEKIAGGAKPEPAPAAVTPAP"
     gene            complement(18130..20922)
                     /locus_tag="PL056_01030"
     CDS             complement(18130..20922)
                     /locus_tag="PL056_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HPM45627.1"
                     /translation="MKCFYCSNVSDIKIKGFCISCGKKLEKICESCDISYEKSANFCM
                     SCGGKLSDLIEKIEEKDDYVEDLPEIKQEDEEIFVLEEVSDDDSVVIETNDISTDKQD
                     IEIEINGSATDEPAGGPELKIEKDSKKASEDSAQSKNESDKRIVSPNAPQNKKSKKEP
                     VQSKGDIDLDSLFNELTSLDGSEQLSAGESSDVPPPEGMSVESVSSNEKDESAFNLKS
                     EKILVKEPDQYFLNPAEVNEKYDVSLDSLINETVTSEFQESCAPMFRKIEERFRICKG
                     GSFYLKGSSGSGKSHIVNRLRSFSEKNAPENGYSIVVSDANIFDFDFMIFIILIKALM
                     KIKSNDVNTVKKKFDKLFGEALPDSKKECLSALICLNFSPVKTKLPKHDIEYLLSYVL
                     YGLSRSKPVLWVINNANTLNVRSIKFFASLNRVFECMPMSVVFVVDPDAAVLGNSVKE
                     NIYEFNGFPEDRLIEEVGVVLNTKKIPADLEKLLKDKASGNILFTVQLAEYLKDRGFI
                     FEMKGSWRFSKLPDDFVCPENLDELISLRVDMLPEDVARTLREFTLLNLYEVPKSLFT
                     LVSTTGASCVEELVKKGYLIETKDHLRFTSRSILTALKKKVKIGKQERAFYKELVSKL
                     ASTPAEIYQLNKHWLLLSYINLGGIVDRRMNSFLFSSAVYMEKLGFFEISQRSYQTIV
                     SSFESDDTNDDFKILPEIKNARLWRIVEPQWAKIFWEKLVTYAKTRSDYHLELVARGE
                     ILLLAEDVDMTTIADIVKKLHMAGCYEEEIGLIDRTTDILVNSGNYLDAVTFATRGYK
                     ILRDVIVKYDGKGVSPAEFVYSLYIRCACKLAEVCIMLNDNDKASAILEEALEYAEKY
                     SVSYFKSKILLLLGKIRYAQKDEWEELMKEGFYNAMIGMDFTIIKAFLHFFEENGFED
                     SEWVSPFIEYKNWINF"
     gene            complement(20976..21974)
                     /locus_tag="PL056_01035"
     CDS             complement(20976..21974)
                     /locus_tag="PL056_01035"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HPM45628.1"
                     /translation="MKRFVNSCQLKGKIVRSIVIKCLVFSFFTVSCSESENVQKYTFS
                     GMGTVLSVIYTGQKNDDLEDMLRKDVELIEKEVSYYKPESFVSVLNRDAFFKEVQVPN
                     HVCMLIKTSLEYGKMTDGVFDITYKSEGALWEEGKEPGEEELKEKIKLTGSEMILADC
                     EKNVIKYKKEGVLIDLGGIAKGYAIDRAGEIIKKNGIKDFIVNYGGDMLVCGKKGKEP
                     WKIGIKNPADPSDHLKTLSFGDKECVGIATSGDYERFFVINDKRFSHIFDPRTGKPVV
                     DAKSVTVTAQNALTADVAATAVSVAIKEDELIKKIMEKFKVKIYTLSGSDLKWVEH"
     gene            complement(21928..23289)
                     /locus_tag="PL056_01040"
     CDS             complement(21928..23289)
                     /locus_tag="PL056_01040"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM45629.1"
                     /translation="MRFATENINILIQLVLSVTVIILLFREKRARKAESMRKMFDEEM
                     KKQRDSLDQIVNERTRELRRAMQKAEDANRAKTEFLANMNHEIRTPLNVITGFSYLLK
                     EESKGNEKAVEAVENIEKACENLTNIISDILDISKIEVGKMKLDPDFINIYDLFDEIR
                     KAYMPRSAEKNIVFSVNIDDKMPKVVKIDGTRLRQIIHNLVGNSFKFTETGEIRVWSY
                     ASVSDIKGCVDITITVKDTGVGIPPEQKDKMFEPFVQQDGQSTRKYGGTGLGLALCRK
                     FVEMMGGTISVESEPGRGSVFFVSIKGVEVSKDLVRSELKRLSESSKSVRFNGEKVLI
                     AEDNIMNREVLKNFLSASNLNIFEVSNGRDAVSVAKNIKPDIILMDILMPDLDGLSAS
                     KVIRNNAELKHIPIIAVTALAGRDQVPEILRVCNALIEKPVRKEVLIEKICEFLPVEG
                     KDS"
     gene            complement(23349..>23815)
                     /locus_tag="PL056_01045"
     CDS             complement(23349..>23815)
                     /locus_tag="PL056_01045"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPM45630.1"
                     /translation="FNSVFQPFIVMITLPLAFIGVAIGLWVSGLDLSMMAMISLVALA
                     GIVVNDSIILVDFINNLSSVKDERTRRDILVEAGTKRLRPILLTTVTTVGGLIPMAVF
                     ATGSNKMWQPMAITIIWGISFATILTLFVIPVIYAVIDDIKKSVYKLFGKKA"
BASE COUNT         6711 a         5481 c         4023 g         7500 t
ORIGIN      
        1 tgattaattt tgcttgacgg ataccttttt tcaagtaaca ttcttttgtg gatttatcca
       61 cattcccctc ccaccccaga tatgaggcgc ctttttttag gcgccttctt tttttatcct
      121 gttttgagag ccctgttgat tgatttttcg ttatttatcg caattatctt tccagccgca
      181 gcattctcag aaacaaaatg cataaattcg cttatcggat ctgtccacca gattttaccg
      241 cactcgctgc atttaaaaaa tcttttatgt ccgttgaaag aaatcgcacg attgtagctt
      301 ccgccattat gttcataaat ttcctttcta aggtggttta aatttccacc cccgcaacac
      361 tcacaattga atagtttgtt cataggaaaa accctcctaa aaaccaaagc acattgctta
      421 tcggtaaatt tagtaacaac tgcgggattt ttttattatt tttttgttat ttttttgtta
      481 actacatgga attcttgaac atcttccact acttgaacaa gtgtatcctg aagggcagtc
      541 agagggtttg acacaatcct gctttccgca ataacctgat cccaggcatt tcataccgtc
      601 gttgcaatca gtatcaataa aacatgtttt aagacagtaa ccgctcacac agtcagaagt
      661 ttcagctccg ccgtgaggac attcagaagg atatgtgcag cccgaacata cgccggaagg
      721 aagaaccgga gaacatacaa ggtctgcatc ttcacaatct ccgctttcag cacagacaaa
      781 gccggaatcg ctcttatctg cacaaacacc ggaagcacaa gtgaaaccat ccggacattt
      841 tgttacagaa accggttcgc actttccatt tatttcctga gttccgtcat cacacataca
      901 agtaggaaca ccgagtttta tctcacatgt acctctgttg atttctttac aagggttcgg
      961 atcgcatgag tttatacatt cgcctgcaat acaggatgcc ccggcatctt cacagcttcc
     1021 gcccgggttt gtttttgagc acagggtctc tttgcacggg atcctttgac acataccttc
     1081 acccgatccg tcagaatcta tacagagagt tcctccgaaa caatcctcat tgctgtaaca
     1141 ttttttctgt ccgcagtaac ctgttccgta acattttccg taaggacaat cggtatcatc
     1201 atcgcacatc agtgcacaag ttccgtatgt ttcataacac tgtgtattcc cggggcagtc
     1261 gcttgctaca gcacatccta tacaaatccc acctgccgca tgaggcgatg aacagacagt
     1321 cgttgcacca gggaattcac ggcagtcacc atcttcaata cactgctcat tcgaaagatc
     1381 gaagggaaca caataaccgc taaggcactg caacccttcc gggcagaaat caaactcaac
     1441 aagaatgcat ccatcttcgt cagcaacata attctcattg caatcacagt gatatccttc
     1501 aatagaaccg tcaagaacac aaacattttt atgaagagga ttgccgctcc cttcacatgg
     1561 attgggaaca catttgtttg aagcaagcgg ttcgcatttg tcatttttga actcaaaccc
     1621 ctgattgcat tcgcaagaac ccgatatgtt tgtcgaattt agaggacagc actttccgtc
     1681 actcatttgc agacctgtgt cacactgaca gaaaggagca tcatttttag ttgaacaagt
     1741 gctgttttca gggcactcgt atccgtaaca acggtcagtg atacaaattc cctgttgaca
     1801 tatttcacct tcaggacagt atccgttaag cccttctatt gtgttttcag agcattcagg
     1861 aacgcactgt tcttcatcgt agtcaggagt aacataatag gtgttgcaga cacattcccc
     1921 ttcaacatta tttgaatacg gctgacagca gagtccctcc gaagaatgat aactcacatc
     1981 acattcacag gtataacttc tgccttcatc ggcaggcatg catcttgttc tgttctcagc
     2041 aatattcttt tccgtttcac atggattggg atcgcaaatg tgatcttgag tatcttcatc
     2101 atccgattcg ttatcactta ctgaagaact gtcatcatct aagacaacat cgtcatttac
     2161 atgaccgctc ttgtcatctg tctcattatc actattttcg ttttcggata atggatcctt
     2221 atcacttatg aagttatcag aatcactgcc cgttgaagtc tttgacgaac aggaaaaaaa
     2281 tccggctgac aacatcatca gcaataccag acactttttc attacaaacc tccttgtacc
     2341 agttctatat ttattttaaa gaacttggtt tctccatcat atccatttga tttcacttta
     2401 tatttattgt catcattaac aaaagtcact tcaagtttta actcaggtgc ggtctgaggt
     2461 gttacaggat ttgtaaactt aaggtcagca ccggtaagta cgacagaaat tgaaagaaat
     2521 gtttcgaaac actctttaac catctgcatc agaagaacac ccggaacgac tggcttttca
     2581 ggaaaatgcc cattaaaaac aggatgtttc caattaaaaa agattgtggt tctgaatccg
     2641 tcctctattc tttcaaattc cgaatgggta aaaagatcat ttctgaacat tgttcttttc
     2701 caaaagtgac agttcaattc tgagcttaac attaaaatga acgatcgtta taaatgcagg
     2761 agtgactgaa cctgattcat tgtagccgta atttatttca aaaaccggtt catcaatgtc
     2821 ttcggctttc catacttttt tcaacaggcc ccctttcttt tcgatcagaa aattgttgga
     2881 cagtttttca cttacagcac attcataaag aaaatcttca ctatcctcga atagatatcc
     2941 gttacagtca ctatgctcaa tcccgcttag aaccatcgtt gtaaagtctt ctctgaatgt
     3001 ctttttcact tcagacctgt caagttcagc gaaaacattc tgcatttcag caattccgtt
     3061 tttgatctcc atatcaaaaa gcttaaaacc ggtttctgag atcagcgaca ttctgaaatg
     3121 atctttttca agtttcttaa ccgcaagaag tcctgaaaaa tacatcgaat acagctttat
     3181 ggaaactctg tacaaagcag aagttgtatc tgaaaaaggg acctgtgata aattcagaac
     3241 cgtcttgcct tccacaacac aattacggca tggaagagtc acacaggatg taaaaagcat
     3301 caacagcagc aataatgccg tgagtttatt taacagaaaa ttccgattca tctattttac
     3361 cgttcaactt tttgtcaatg aaaagaagtt ctgttgagtc acctgttatc tcgaccattg
     3421 taagcatgct cacagtaaaa tccgatttat ccattatcat gttgatattt ctgaaatatt
     3481 ctgacaagga tgttcctttc actggtgtca tggaaatagt cagttccctc cctttttcct
     3541 ttatatcaaa aataaaattt cctttgtctg ataatatggt tccattcaaa gaagcaacca
     3601 ttatttcgtt tatttcctgg aaaaccctgc tggtgctcat atcaaagctt gatattttct
     3661 gcccgtcttt catggttata ttgtttccat ttattacaat cgtgtagaaa aacggagttg
     3721 tatattccca ttttaacttg ttaccattct cactcttttc aaaaaagaat ctgccgcctg
     3781 aagtcacttt ctgtgaaagg aatgacagtg ttttcacttg cttgaaagaa ctttttattg
     3841 aaacaatgct ggatgcattt gatgcaacag tatttcgcca ttttgcaaca tttgaagacg
     3901 aggcaagttc tgtaaaagcc gcactggcgt cactttttac attttctgta tttactgttt
     3961 gtgcaaaaga cagcataaag aacagcgtta aagctataaa taatatttcc ttcaatcaat
     4021 cctccagaaa aaacttttca ggaaccgtca gttcaaaacc ttccttttga cagtagacaa
     4081 tgaattcatc aagcatttca accgttcctt taaccgtatc atgaaaaaga acaatgtccc
     4141 ctccttttac ttttttcatg acagatgaca atatttttcc cttttctttc aaaaaagtat
     4201 caaacgatct taccgaccag ccgatagtta ttaaaccgag ttcccttgct gcataagcta
     4261 ttgaagggtt tgtaacaccg aagggaggtc tgaaatacag tgttcttttt cctgttatat
     4321 tttcaattat atctgaagtc tttttaagct cgaaaagcat tctttttctt gagtagaaag
     4381 ggaaaaagtt cgagtgaccg tatgtgtgat tgccgatcaa atgacctttt aaaaaaatat
     4441 tttttagtaa ttcaggattt tccaaagctt tcgatccatt tatgaaaaaa agggctttta
     4501 ctcctttttt atcaagtatt tcaagcacta acggcgtgtt aacaggatca ggaccatcgt
     4561 caaatgtcag aaggatgtct ctttccgact ttctgttcct gcacacagct ttcagaaaaa
     4621 tacccgaact tatgctgaaa gatgcccagg caataaatgc aatagatatt atgatgatta
     4681 agctaaatag aaccattttt tataaaaata aatgcatttt ccatattaag aaaactgttc
     4741 cagacaagta cttgttttat ttgagggtcc tttattatca gatcaactgc cataactgtt
     4801 gacaatgctc ctgccgtcat aaattcacct gtcacttcct tgggatttac aatattgatc
     4861 catttgtaac tcttcaaaac atcttttttc cagttgtcgc attcgctgtc accattaaag
     4921 ggagtgaaga tgactgtttt tgcgggatct atgctgtttt tctcaatgaa ctctctgaaa
     4981 tgtttaactg tttgcatttt atctatgaca ggaacggtct taatatctga aacttctgca
     5041 acagatccct cttttttacc tgaaaggaca acaaatcccg ccccttcacc aggtattgaa
     5101 ttttctctgt gaaggcctat cctttcggct atcacaagat attcatttgt catttcatca
     5161 actccgccgc atagaatatt aagttccggc tcttcacaac ttataagaaa cgattcaaaa
     5221 agagccgatt caaaagagat gcctctatgg acaaaagttg aattatatcc ggtgcacccg
     5281 gtaagaatcg aaattgttcc cccgacagtg ttatgggtag aatagataaa aggtgcggga
     5341 ttgagattct cttcgccacc ctgctttatt gaagaaataa attttatcgt gtctgacaga
     5401 catccgagcc ctgttcctgt gattatggca tcaggtttcc tgatcccccc gtttttcaaa
     5461 gctgtcattc cggcaaacac cccattctta attattccgc tgaaccttct tgattctttc
     5521 ggatctataa actcctttat ttccggcttc tgacatctga atgttgtccc gtcttcattt
     5581 ttatgcggaa taagaattcc tgttgatctt atatatgccg gcattttaac accttgtaaa
     5641 tattaagcaa ctgttattgc caccgaatcc gaaggaattg ctcataatgt gttttacggg
     5701 attgtctgtc agagttgaag gaagcggttt gagccctgtt gcttctatcg gtatcctgaa
     5761 cattttatca ccgggaacca tattctcttt aattgaaaga attgagataa cagcttcaat
     5821 accgcctgaa gcgccaagtg tatgacctgt cagtgttttt gtagagctca gcattggcat
     5881 tttttcttta aaaaccctga caaccgctgc actttcagac gcatcgttgt tatcagtgcc
     5941 tgtaccatga agattaatat aatcaacatc ttccgggttt aatcctgctt ttttcaatgc
     6001 accggtcata gccgcatagg caccgttgcc ttcaggactt gaagcggttt gatgatacgc
     6061 ttctgcacag ttactccatc cggcaagttc gcaaatcggg atgtttcctg ttttttttat
     6121 ggtttcctct gtttctaaaa ctgcaaatgc cgccccttca ccaagattca acccctttct
     6181 tgatgcatca agcggtctgc atggctgtgt atcatgaatt ctcaatgaat tgaatccgtt
     6241 gacagtaaag tatgtgagag cgtcactccc tcctgccaca accctttcaa cctctccatg
     6301 ctgaagaagt cttgccgccg taatgaaaga attggcggaa gatgaacagg cggtacttat
     6361 tgttgtaaca aaatctgtcg catttataag aacagagact ttatgtgctg tatcgccgca
     6421 atcatggcag ataacatcat taatatctcc gccgttttga agatcgtttg aaaactcccg
     6481 gtaaaacagc tcgcttctat ccattccgcc aactgttgtt ccgcagacaa gacccgtcag
     6541 tatcccgtca tttttcctta tttttgcatt cactgccgct tcttttgccg caacagcagc
     6601 aagaagagag caccttgttt taaattcatt tcttgaaacc cctgcaattt gagcaagttc
     6661 gtcattgctg aaaggaactt cgcaaagtgg aaaattcctt gcgagctttg aatcgacaaa
     6721 tcttgtttct gtctggccgc ctttcatttc aacaagcgat ttgaccgtct gaccacagcc
     6781 gatgcctatt gaacttatta ttcctgcagc agtaacatat atcttcttag ccatcttatt
     6841 cctcttcgtt atcgctgcct ttgggaaaaa caacatgctt tcttgcgaat gccgtttttt
     6901 ccctgtcgta accttttgca actattgaga tgctgttaat cttcttttcc aatgtcaccg
     6961 gcacgctgat cttgtttaca ctgcctgttt ctgaaagtct tttataaaaa tactttttgt
     7021 catttataaa aacaaagaca tctcttgttt cagcagggtt tatctcaaat gctatattga
     7081 atttcccgtt ttcagaaagt gaaggcgatc tgtcaaagaa aattccgggc attttaatac
     7141 tgtatttaat gtccatttgc agttttgacg gtttttcctc tgaagtcttc acattttccg
     7201 accttatcca gaaaccgtct tcagaaagat aggcctctcc gcattttccg gtacttttca
     7261 gaacggatct ctcttttatg atgccatata catttttcag atctgcggca ctgaatatct
     7321 ctacaccctt ttcaagaact atccccgctt ctctgctttc aaatacacat cctgcgcctg
     7381 acctgtatat cggaactgaa aaacctgagt tgtatttcgt tttgtaatca tacaaagaca
     7441 ccgcaagctt tgttgccaga gtttcttttg cctcaaaatt actcaacttg aatttaaatt
     7501 ccgcactttt tgtttcaccg ggagcaagag taaaagaaag agttccgctg ttaatgaata
     7561 tctcttttga attatccaca tttatcaaca tcgcccttcc gtcaagaaca gcccctttcc
     7621 cgatgttggt aaaattgatc agaagcttaa cctcttcacc tggctgtatc ataccgtcaa
     7681 cacttccacc tgagtcaacg gatagaaaag agagctttat atcaggtgag tcaaccggca
     7741 tggtctcaat aacaaaatct ttgattttaa ttactttgtc aggcgttcct gtgtaaagct
     7801 ttaactgtgc gttctccttt ctccacggga ctgactcagg aagctttatg ttcacggttc
     7861 tttcgataga accgctgaaa gtaccgaaag gaatttccag tcttcttaaa aaaggaatat
     7921 ccgtttcaag aactcctatc agattctcta tttcaccctc gatctccgct ttgaacttga
     7981 actggtaact gcttccgcca atgatcttct gactacttaa aagagacact ttgagtgaat
     8041 ccaggtcagg ttgccgcaga gacattgatg atttccacgg aatacccgat tctgtcagtt
     8101 ttgaaactat tcccgaccac tcgctttcct ttattctgac agcggcatcg ttcggtttga
     8161 taccgtttga tatcatttct ttcatgatct ttattgctat ttcgatctcc tcgtcgcttt
     8221 taaaagcacc gatcttctct tttctcctct ctttcactat ttgatcagta tctttgtatt
     8281 ctttgaaata cctgacaatt atatccggtt tttcgggctt ttgcggaata taagaactga
     8341 cgatatgctc tttaagcttg ttctctgtaa ctatgttctc gcccatatca aacaacgata
     8401 tcttttcatt gtgaataaaa gccggcctta tctcgatttc aggagttatt cccacagatt
     8461 ggatcgaaac acctcctgga gtcagatact gggcaattgt aagcttcatg cctcctccgc
     8521 ccggtatcct gaaaaggttc tgaacactcc ctttgccgaa actttttctt cccagaacac
     8581 tcgctctgcc gtttttctta agagccgcgg taacgatctc ggcagcagat gcggtgcctt
     8641 cgtttatcat tacagtcaca gggatgtcaa gaatgtccgt attctgcttt tctgcttcgc
     8701 tgacatcatc ttcatcatct cctacagtag aaacaataac ccccctttcc agaaaaatat
     8761 cactgatctt tatcgcctgt gaaagaaggc cccctgaatt gttccgcata tcaagaataa
     8821 gcgatttcat cccttttttc ttaagatctt ccaacgctga aagcattgac ggataagtgt
     8881 tttccataaa accggagagc tttatataac ctattccggg ctccggcatt gcagaaacga
     8941 cagactctat tttgatctct gctctggtaa ttgtaaattt taaaaggtcg ggaacaccct
     9001 ctcttctgat ccttatggtg acgctggttc ccggcttgcc tctcattctt tcaactgcct
     9061 tgttgagtgt catgtttatt gcagattcat catcgatctg cactataaca tcgttagctt
     9121 ccacccccgc ttccattgcc ggagtaccgt ccatgggaga aacgacaacg atctctccgt
     9181 catcattaac acttattacg attccgagcc cgccgaaatt tccttccgtt gagcttgtaa
     9241 actcttcaaa ttccttcggt gtgaaaatat atgaatgggg atcaagcttt ttaagaatgc
     9301 cgttgacagc aatatactct acatctgtta tttcaagaga agcgtcggga acataatttt
     9361 tttcaataaa gccgtatacc tgcttcaata cataggcaat atcataaaga tctctcattc
     9421 ttcccacatt agccgtgtag gtgtgattat atatctggac tgtaacctga tttctgttcc
     9481 tgtcgtattt gatcaatgtt tcaggtagct gatcatgtat cgcattcagc ccttcaacca
     9541 gaagatccgc ggcctgcaac ttagatttgt taagatattt gttcttaaca gccctcaaag
     9601 cataaattgt cacttctccc tgcgatatgc tgtaatcctt tgatgttgag aacatccttt
     9661 cgaacaggtt ggcattaaga ataaatgcaa aaagaaggat cagcgcaact gcaatttttc
     9721 ttttcatatc catgactccc gacacatttt ttatataatc atctctcaaa atctgaaacc
     9781 taaaagatca gtaataacaa aatcaaatct gcccatcaac aggatgatcc tcgaaacaag
     9841 agtatatcca aaagaaacac ctattgcaac aagcagataa aatctgcccg tctcccctgc
     9901 ggcagacaaa aagccgttaa cctttctgtg ccttgcaaca agaaaaagaa gaacggaaac
     9961 aataccggta atacttacaa ttgcatttat tgtaaggtcc caccttatac ttccgttttc
    10021 agctaatgaa ataagcggga tgctggaaga acggatcatt tcatagacat atgcctgaaa
    10081 atagacaggg atgctcgctg caagatagag catgacaata aaaacagacg gcatgaaaat
    10141 aagttttgaa tattttctct ttccgtctga aggaataaaa agcagaagtc ctgatatgac
    10201 aggaaccaga agaatataat caccgctcat tgtatcatca aaaaccggaa taagaacaga
    10261 aaaaagccat atcgatgcaa aatatccggc cgacacacca atgaatacgg actcagcaaa
    10321 tttatagacg gggttgtcac ggtaaagacc tgagaatgct gctaaagtga aagcaaacgc
    10381 tgtccagacg ctcaaattca tacttatgtt caccgttcac ctctcacaag ccgtcttatc
    10441 cctgaaagaa ctatgataat aagtatatat tccaatatca tcgacattat gtaatacctt
    10501 ttccagatat ctgatgaggg gtcaatcacc tttgtcaggc ccgcaatctc tctgaacccg
    10561 cctgcaaacc cttcaaaaga accggattta tgaaaaggaa gatagttcgg gaccatgtct
    10621 gatgaacaga tgactgcctt tgcaagattt tcatctcccg acagaacaga aaaaagtgtc
    10681 attcccggtg agccgtcgat cattctcgag gagaactcaa aaacagccgc aaaatcatta
    10741 aaacgctcta tcccgttcat aataggaagt gccttcagtt cattaccgta aatatctttt
    10801 ttccttacgc cggatatatt tgatgaaagc agaaaagctc ctaccgtgcc gcccccgatg
    10861 tatccgatat ttacatagtc atatccgtag agatatctct ctttaataaa atcagggttg
    10921 ccgatacttc tttcaaccgc catgaatgct gactccacac ccacaggaac gagagtcgca
    10981 aaaactatac cggcatcctt tgcagcaaaa tatcccacca ttgcagaaag agtttcctcc
    11041 agctcatatt tagcttccgg accgaaattt gtaattataa gcacttttga accggcagga
    11101 acattttcca ttatttcata tacctttttt ccgttatctt ccatacccgg atcgatttca
    11161 gtatctataa atctgaaaat gataattata aaaacgggta taaccgccag atatttaaaa
    11221 ggaatgtcga ataataaaaa cccgatcctt ctcaatttcc cctcctgttt ttgggtgtaa
    11281 atacttcaaa aacagtttct gcggaataaa aaagaacccc tgttccggtc cctattgcaa
    11341 gtgccttaaa aacaggtgct gaaaaaacat ttatcaacca cccctccgaa aaaggaccct
    11401 tccagtcagg acttaagaaa aaatttaaaa ctgaattaaa ataaggaaag aatccggcaa
    11461 ttacaagaca cacagacaca gatatgacaa aatagctgta atttctgaaa tcaagcattt
    11521 tatataccgt caaaagcatc gaaaagaaca acatcgctat aaaagttgaa aaaagaggat
    11581 caaggattga ccggtatatc caccttacga aacccttcat attcgggctt gatgtaacgc
    11641 cggtttttaa aaatgctgaa aacattttag atgcggcatt ttttgaaata tccatatctg
    11701 cattcttaga acagctttcg gctattatgt cagcaacccc tgaacttctc acaaaatcag
    11761 cgtagtattt aacagtttca tttttattta ttcctgacac attctgctca tcaaaccatt
    11821 ctttaagcat atcccctgac cctttgatcc ttactccttc tattgcagca tcctgtacat
    11881 tatcctgttc acctttttct cttaaagaat ctgtgaacca ttcccttaaa gaagcagcaa
    11941 caggaagcat ttttgaaatt ccttctattg acatagctct cacttttttc tcgagctcgg
    12001 aaaccgattt tgaatcatct tcatttatga aaacagtgtt cttttcatca aagatccctt
    12061 ttttgtattt aaggtcctta aaatcttcgc ttgtcatgtc ggcttccttt aacatttcat
    12121 ccgtaggaaa aaaataaggc gaaacattgg cactaacata aagactcagt ttttttcttg
    12181 cagggaactt gctcagatag taatcatcgt cgattattat gtctgccgtt tcaacaaggt
    12241 atgccggctg ttccctgata atattgttaa atgcatattt gctctggttg atattcattg
    12301 cacctgcaag gaaagtaacc aggaacgaaa gcagaatgac tgctgaaggc cactgtgaaa
    12361 atcttccttt ccttatcttc accgcctctt tgtgaagcac ttttaacaga cccgcaacaa
    12421 aagaaataac aataaccgtt tccccgcacc ttaaaatgac agaaaatata tttcgcccat
    12481 aatcagcata aatcaccaga acgctgaaaa ttacaagcgg tattaaaatt gtgatcactg
    12541 caaatcttct catattccgg cctgaatgtt aaaaagttca gtaataaacg gaaaatattg
    12601 cgcaagagta ctcaatactg atccggcagt tataatgaca gcagtaatga tcagaaaaag
    12661 gtatgctccc gacaccttaa ccgatctttc agacctgatg ttcttaagct gggaagataa
    12721 aagaaagggc tctattgaaa taagattttt ccttgacagt atagcattgc cgctcaatcc
    12781 cggaacattt aaagaaaggt ttatgttccg tttactttct cctgcaaaat atgtgctcca
    12841 gtctgagatc gcagatccat cggaaatatt gaaacccatc tgcctgctcc aatcctgcct
    12901 gttcactgca gaataaaacc cggccagatc accgaatgaa ggcactgcga caaaactgtt
    12961 cctgccgttt tgaaaaaagc tgtcgctccc gtccggaaac agctccgact ctattgatga
    13021 gccagtatct gccgaatttg caagacctgt cattaatgcc gtcaaaggcc caagaagcgg
    13081 atgccccgtt gaatccaccg agctcaaaaa agatattttt gcggtattct ctccgagaag
    13141 agttacaata aacttaagca ttgattgtct gtcccggctt atcgaactga aaaaaggtct
    13201 tttgataaaa ccggctgcca atatggaaaa aataaatata aaaacaacaa aaaccattga
    13261 aatgtttatt ttcttcagaa tatccgcctc tgaatgacat tttcttgctg tttccctcac
    13321 cgttccgtca tcataatata ttttgagaaa ataacatgcc cttattgcgg aaggtgctga
    13381 aaaatggttg tcaggaaagg tgaactcata aatattgaca ggcggagagt tccttaaaac
    13441 cctgtaaagt tcgacattta caacattgct gtcctgaata ttcacgacaa tatctatctt
    13501 atcattttca ttatgaacta ttttaaaaga agatccgtct tcagccagga tcataaaata
    13561 aaacatgctt tggaaaagca tcgcaaaaac aatctttttc atcagcttct ttcttttctc
    13621 agatcaaaaa ctatcgtcct tccggctttt ccggtcttta agactttctt cctgccggca
    13681 ccttcaaaat agactttttc agatacatct atctctatta ccgaaaaagg atctattttc
    13741 attacatatc tcttccccca cagaaaactc atttcatttc tctcttcatt accgctgaca
    13801 taaaccgtaa gacatgttct ttcctttaca aaagttccat ccggaataac tatccagccc
    13861 gaaaccatgt tttctgaata cgatgcatca agaagccatc cggaatgatc cccttgtttc
    13921 tcaaagaaca tcggggctga agcaagaaga actccgtccc tgtcaaaaac aaattctatt
    13981 acgcctctta aaagatcggg atcgcttaca acatcaagaa acttttcaaa acccgctttg
    14041 ccgcttaaag agattcctat gatcctctca ggacggtaat actcaactct ttcacttttt
    14101 tctatctgca aaagatcttc ttgtgcaaaa tccctgtcag gaagagattc ctgctgaaaa
    14161 atacttctga gactttcaga aagatctgcc gttttcaaag aaacatcgtc cattgaaaga
    14221 ccatatctct tgaaatggaa cttacctgct gaccggctac attcatttac aacagaatat
    14281 tcttccgtta taagcaccga aagaacttta tttacatatc ttccgcaaaa catttccgta
    14341 actttctgca aagaatttga aaatgaaaat gcagtcacct tgggttgatc tgaagaaatc
    14401 atcaaagttg aagatctttt tatcgaaata tcttctcctg caaccagact ttcaggacag
    14461 aaactggttt cgagcacatt tggaagcatt gtaaaatcgg ttaaagtgcc gatgttgacc
    14521 tttgcaaaag gaagtgcgga tgaactccct gcaaaaacca cggcaggttt tgtctttttt
    14581 ataaactgac tttcggtaag tttcagaata aaatcatgca gctgtctatt ttcagaatca
    14641 tcataaccac ctgatatgat aagaacatcc tcactgcatg agtttatgtc agattcactc
    14701 atttcgttta taaggaatgt cttttttata tcaagaagca tagaccttgc aagttttaca
    14761 caagcatgca gagaaccttc agaggtcatt cccgtaacaa cagccgaaag cagttttgga
    14821 ggaatgctca tcatactcga agaaactttg gatggctgat cgctcttatg gtaactgctt
    14881 tcctgcatta attttctgac atgttcctta aaattgaaat tatcaccctt ttcaaaaaca
    14941 tcttttacaa aaaatgccgt tccattatct ccgtattcaa ccacaactgt tcttattttt
    15001 gactgcatta caataaatga aatgtgatag cttttcattt cagccctttt tcaagcgaat
    15061 tctttaattg ttcaaaaaag gtctcatcct caaatatttt ttcgatcatt ttgcctcttt
    15121 ccagtccgaa aacgatcgaa aacagtggca gaaaaacagg aagtgtgaaa agtattattc
    15181 ttttgaatgg tcttgaagca ttaagaagaa gcaatgccgt ggactgatct ttcataggca
    15241 gtcatcatat aaaattggct ctcttttctt aagaaattca gaaattacag caagagatct
    15301 gctgtcaagt ttgataaact tcagtcccat acccggctct tcaccgctta tcattgcgct
    15361 atcttcgcct cttacccaga caactagaga atcgatctct aatttttctt taccaagaaa
    15421 aagtatcatt ctgaattctg ttccgatggg aaatatctga tgagtagcca caaaaacacc
    15481 accttcgctt atatccattg tcaatccgct gaaaaaagta tgcggacctg taagcgttac
    15541 ctctatttca acatcaattc tctgtgccgt cctctgattc cgggaagaaa acacaacttc
    15601 atcagcattc ctttcctttt cttcgaacat tacctttttt gctattcctt tactgttatc
    15661 aacggccatt ttcagaaaac ctcctcaatt tcaacaacaa tcactgttcc agaacgaatt
    15721 ccactcttct gtttttagct ttgccagcag ctgttctatt tgaagaaacc ggtttgtcag
    15781 gcccatatcc cttcgcttca agcttgccgg aatctacacc ctcgtcaaca agaaattctc
    15841 ttactgcttc agctcttttc tgagaaagag atatattcag gttcctgctg cctacattat
    15901 cactatgtcc ttcaataata acttttttta cagaaggggc atttttgagt gcatcggcaa
    15961 cttctttaag catctcatat gacttcctgt caatgactgc cttacccgtc tggaaaagaa
    16021 caacctgttt aagttcaatt ttctttgctg taacaacaat gagtttatat tttttcggac
    16081 atcccttttc ttcctcgact ccgggaacat caggacattt gtccacatca tcatttattc
    16141 cgtcaccatc cttgtcaagt acctgtggac aaccgttaag ctcaacggta cccggaacat
    16201 ccggacattt atcatcaaga tcagcaacgc tgtcaccgtc acggtcagga caacctttaa
    16261 gagtcgttat ccctttgtca tcagggcatc ggtcttcaga atccgggata ccgtcaccat
    16321 ctctatcagc ggcaggagga caaccctgaa gttctttcgg acctattgca tcaggacatt
    16381 tgtcatctac atcagcgatc ccgtcaccat cacgatcagg acaacctttg aattcagcag
    16441 ttcccttgtc atcgggacag cggtctgtgt tatccggaat accgtcacca tcacgatctg
    16501 cagccggagg acagccgttc aattctttca aacctttttc atccgggcag cggtcatccc
    16561 tgtcaggaat accgtctccg tcacgatcct tggccggagg acatcctttt agctcaacga
    16621 caccagggat atcaggacat gagtcatcaa gatctgcaag tccgtcacca tctctgtcag
    16681 ggcatccttt aagttcagca aaacctttat catcaggaca acggtctgtg gaatcaggaa
    16741 caccgtcacc gtcacggtct gcagcgggag gacaaccatt caggccgaga ggtccggaaa
    16801 catcaggaca tttatcatca atatcaggca gcccgtcacc atctttatcg ggacaacctt
    16861 tgaattcagt tgtgccggca tctttggggc atctgtcttc cacatcaagt atcccgtctt
    16921 catcagcatc tttaggaata cagatatccg catttaatga agcttttgta gcataaagat
    16981 ctttggccgt tatgtgttct tccgcacggt aatgattgcc tgactccagc tcgagtctgg
    17041 caaattctat gtggctttcg gccattgcaa tatcatacgg ggcgcaaata tctgcaccgc
    17101 ggtttttatc gacttcaagc cgtttgctct caagcagcgt gcttttgtaa agtttgcttc
    17161 ctgcaacgca tccgccaaga agcataaaag atattgctat tattatatgt cttttcattt
    17221 tgcacctccc ttcggagcag tgctgtcttc ttttttcttt tcctttttct gttcctcttt
    17281 tttctcttcc tctttttctt tttccttttc gtcagactgc tttttcaaat atttttgtct
    17341 catttcttca taaacatcat agtaagaagg tttcttttcc tctttctttg ccggctcagc
    17401 ttttttcggc tcctcttttt tttctgcaaa aggatcccct ttcaatgagg gtttttcttc
    17461 aggatccgct gttacagcag gttgcggctc cggttgcggc tccggttcca gcttcacttc
    17521 aggcgccggt gttnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnggtgcag
    17641 gggttactgc tgcaggggca ggttccggct ttgcccctcc tgctattttt tctgcttcgt
    17701 tgaaatattc caatgctttt gttgcaagaa aatcggatga atcataaatg ctttttgacc
    17761 taaaatcata agctttggaa agatatgcat cacccttgaa gaaatagtaa gccgcctttc
    17821 ttttttcaag cggtttaact tcatccgcag ctttttttgc cagcgatctt ctcccgaatt
    17881 catcggtttc gactatgtcg gccttttcaa atgcggtggc gattttacca agagactttt
    17941 cccgctggtc ttccgctttc catattgaag aagtagatct ggcgatcccg catgatacga
    18001 atattaccgg tatcattgca aaaaaaacaa ctgacactaa tctcataacg acctctgcct
    18061 aataattagt gagaaaagtt accagaaata tctggctcag tcaagtttaa atctgcatga
    18121 aaataaagat tagaaattaa tccagttctt atactcaata aagggggaga cccactcgct
    18181 atcttcgaaa ccgttctcct caaaaaagtg gagaaatgcc ttgattattg taaaatccat
    18241 tcctatcatt gcattgtaga atccttcttt catcaactct tcccattcat ctttctgtgc
    18301 atatcttatc ttgcccaaca ggaggagaat ttttgactta aaataagata ctgaatattt
    18361 ttctgcatat tcgagagctt cttccagaat cgcacttgcc ttgtcattgt cattgagcat
    18421 tatgcatact tcggcaagct tgcatgcaca tctgatgtaa agggaatata caaactccgc
    18481 aggcgaaaca cctttcccgt catattttac aattacatcc cttaaaattt tgtaacctct
    18541 tgtggcaaaa gtgactgcat ccagataatt gcctgaattc acgagtatat ctgttgttct
    18601 gtcgataagc cctatctcct cttcatagca accagccatg tgaagtttct tgacaatatc
    18661 tgcaatggtt gtcatatcaa catcttcggc aagaagaagt atctcacctc ttgcaacaag
    18721 ctcaagatga taatcagatc ttgtctttgc gtatgttaca agcttttccc agaatatttt
    18781 tgcccattgc ggctcaacta tgcgccataa acgggcattc ttgatctcag gaaggatttt
    18841 aaaatcgtcg tttgtatcat cagattcaaa tgatgaaact attgtctggt acgacctttg
    18901 agatatttca aaaaatccga gcttttccat ataaacagca gaagagaaaa ggaatgagtt
    18961 catccttctg tcaacaatac cgcccagatt tatatagctt aaaagaagcc agtgtttgtt
    19021 aagctgataa atttcagcgg gggttgaagc gagtttcgaa acaagctctt tatagaaagc
    19081 cctttcctgc tttcctatct taaccttctt ttttaaagct gtgagtatcg atctggatgt
    19141 aaatcttaaa tgatccttgg tttctatgag gtaccctttt tttacaagtt cctctacaca
    19201 cgaagcaccg gttgttgaaa ccagagtgaa aagggacttc gggacttcat aaagattaag
    19261 aagcgtaaat tccctcagtg ttctggcaac atcctcagga agcatgtcaa ctctcaaaga
    19321 tatgagctca tcaagatttt cagggcatac aaaatcatca ggaagttttg aaaaacgcca
    19381 acttcccttc atttcaaaaa taaatcctct gtcctttaga tattcagcaa gctggacagt
    19441 aaaaagtata tttcctgatg ccttgtcctt gagaagtttt tcaaggtctg cagggatttt
    19501 ttttgtattt agcaccacac cgacttcctc aatgagacgg tcttcaggga aaccattgaa
    19561 ctcataaata ttttccttaa cgctgttccc gagtaccgcc gcatcaggat ccaccacgaa
    19621 tactaccgac attggcatac actcaaaaac cctgttaaga cttgcgaaaa acttaatcga
    19681 tcttacatta agtgtgtttg cgttgttgat gacccagagc accggtttgc ttctgcttag
    19741 accgtaaaga acatatgaaa gaagatattc tatgtcatgt ttgggcaatt tggttttaac
    19801 aggtgagaaa ttgagacaga taagagcact caggcactct ttcttgctat caggaagagc
    19861 ttctccgaac agtttatcaa atttcttttt tacagtattg acatcgttgc tttttatttt
    19921 catcagcgct ttgataagaa tgatgaaaat cataaaatca aaatcaaaaa tgttggcatc
    19981 tgaaacaaca atactgtaac cattctcagg agcgtttttt tccgaaaaac ttctaaggcg
    20041 gttaactata tggctcttgc cgcttcctga actgccttta agataaaatg agccgccttt
    20101 acagatacgg aatctttctt ctatctttct gaacatggga gcacagcttt cctgaaattc
    20161 agatgtcact gtttcattaa tgagagagtc gagagaaaca tcatattttt catttacttc
    20221 ggccgggtta agaaaatact ggtccggctc cttgacaagt attttttcac tcttaaggtt
    20281 aaaagcactt tcgtcttttt cgtttgagga aacagactcg acactcattc cttcaggagg
    20341 cggaacatca gaactttctc cagcggaaag ctgttcagaa ccgtcaagtg atgtcagttc
    20401 attgaaaagt gagtccagat ctatatcacc ttttgactga acaggttcct ttttcgattt
    20461 tttattctgg ggagcattcg gagaaactat tcttttatca ctttcattct ttgattgtgc
    20521 tgaatcttca gacgccttct ttgaatcctt ctctatctta agttccggtc cgcctgcggg
    20581 ctcatctgtt gctgaaccgt tgatctctat ttcaatatcc tgtttgtccg tagaaatgtc
    20641 atttgtttcg atcacaacac tgtcatcatc tgaaacttct tcaagaacaa agatttcctc
    20701 atcttcctgt ttaatttcag gcaaatcctc aacatagtcg tccttttctt caatcttttc
    20761 gatcaggtcc gaaagtttac ctccacatga catacagaaa ttagcacttt tttcgtaaga
    20821 gatgtcacac gattcgcaaa tcttttccaa tttcttgccg caggagatac agaaaccctt
    20881 aattttaata tcagacacat ttgaacaata gaagcacttc atcgtatagc cctgatttta
    20941 ttaacttaat ttcatcatca atttcttttc caagcttaat gctccaccca cttcaaatcg
    21001 gaaccggaga gagtatatat ttttacttta aatttttcca taattttttt tatcagctca
    21061 tcttctttta ttgcaactga aaccgctgtt gcggcaacat cagctgtcaa agcattttga
    21121 gcagttaccg ttactgactt tgcatcaact acaggttttc ctgttctcgg atcaaatatg
    21181 tgtgaaaatc ttttatcatt tatcacaaaa aatctttcgt aatctccgct ggttgcaatg
    21241 ccgacgcact ctttatcgcc aaacgaaagg gttttcaaat gatctgacgg atctgcagga
    21301 ttttttatcc ctattttcca aggttctttc cccttttttc cgcaaaccag catgtcaccg
    21361 ccgtaattta caataaaatc ttttattccg tttttcttaa ttatctcgcc ggctctatct
    21421 atggcataac cttttgctat tccgccaaga tctatcagca ccccttcttt tttatatttt
    21481 ataacatttt tctcacaatc agccaaaatc atttctgaac ctgtaagctt tatcttttct
    21541 ttgagttctt cctcccccgg ctcttttcct tcttcccaga gagcaccctc gcttttatat
    21601 gttatgtcaa aaactccatc cgtcattttt ccgtattcaa gcgaagtctt tatcagcata
    21661 catacatgat tcggcacttg aacttcttta aaaaatgcat ctctgttgag aactgaaacg
    21721 aagctttccg gtttataata actaacttct ttttcgatca gctcaacatc ttttcgcagc
    21781 atatcttcca agtcatcatt tttttgaccc gtataaataa cgctgagcac tgttcccatg
    21841 ccggaaaaag tatatttctg aacattctca ctttcactgc aggaaacagt aaaaaaagaa
    21901 aaaacaagac attttataac tatcgatcta actatctttc ccttcaactg gcaggaattc
    21961 acaaatcttt tcaataagca cctcctttct gaccggtttt tcaataaggg cattgcagac
    22021 ccttagaatt tcaggaacct gatctcttcc ggcaagtgcc gttactgcaa taatcggaat
    22081 gtgtttcagt tcagcattat ttcttatcac ttttgaagct gaaagcccgt caagatcagg
    22141 catcaggata tccatcagaa ttatgtcggg tttgatgttt tttgccacac tcacagcatc
    22201 acggccgttt gaaacttcaa aaatattaag attggaagca ctgagaaaat ttttaagcac
    22261 ctctctgttc attatgttat cttcagcgat caggactttt tcaccgttga atcttacact
    22321 ttttgaactc tcactcagtc ttttaagttc agagcgcacc aagtcctttg aaacttcaac
    22381 ccctttgatt gaaacgaaga aaacggaacc tctgccgggt tcactttcaa cgcttatggt
    22441 tccccccatc atttcgacaa atttccggca aagtgcaagt ccgagccctg ttccgccgta
    22501 ttttcttgtg ctctgtccat cctgctgtac aaacggttcg aacattttgt ccttctgttc
    22561 aggaggaatg cctacaccag tgtcctttac cgttatcgtt atatctacac acccctttat
    22621 atccgacact gatgcatacg accagacgcg gatctctccg gtctcagtga atttaaatga
    22681 attgccgaca aggttgtgaa tgatctgtct taatctcgtg ccgtcaattt tcaccacttt
    22741 gggcatcttg tcgtcaatat taaccgaaaa aactatattc ttttctgccg atctcggcat
    22801 atacgctttt cttatctcat caaaaagatc gtaaatgttg atgaaatcag ggtcgagctt
    22861 cattttaccg acctctattt tcgaaatatc aagaatgtca cttattatat tcgtgagatt
    22921 ttcacatgct ttctcaatgt tttcaacagc ttccacagct ttttcatttc ctttcgactc
    22981 ctctttaagc agatatgaaa aaccggttat gacattaagc ggcgtcctta tttcatggtt
    23041 catgttagca aggaattcgg ttttagccct gtttgcatct tccgctttct gcatcgccct
    23101 gcggagctcc ctcgttcttt cgttcactat ctggtcaaga ctgtccctct gctttttcat
    23161 ctcctcatcg aacattttcc tcattgactc agcttttctc gccctctttt ccctgaacag
    23221 aagaatgatg accgttacac taagcaccag ttgaattaaa atgtttatgt tctctgtagc
    23281 gaatctcaaa acggaacctg ctaaattttt cgggttatct tatcaattaa tcgggaaatc
    23341 tcaatttttc aggccttttt tccgaacaat ttataaacag atttcttgat gtcgtctatt
    23401 actgcataga taacgggaat gacaaagagt gtgagtatcg tggcaaatga tatgccccag
    23461 attatggtga tcgccatggg ctgccacatc ttatttgatc cggtcgcaaa aaccgccatc
    23521 gggataagtc cgccgacagt ggtaacagtt gtaagaagta tcggtcttaa cctttttgtt
    23581 cctgcttcga caagtatatc ccttcttgtc ctttcatctt tgacactgct taagttattg
    23641 atgaaatcga ccagtattat tgaatcattg acaacaattc cggctaatgc gacaagagat
    23701 atcatcgcca tcattgaaag atccaggccg gaaacccaca aacctatcgc aactcctatg
    23761 aacgcaagcg gcagagttat cattacaata aacggctgga aaacgctatt gaact
//
LOCUS       DAOOYB010000008        22700 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_2106_length_22700_cov_12.380835, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000008 DAOOYB010000000
VERSION     DAOOYB010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 22700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22700
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2106_length_22700_cov_12.380835"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1085
                     /gene="gcvT"
                     /locus_tag="PL056_01050"
     CDS             <1..1085
                     /gene="gcvT"
                     /locus_tag="PL056_01050"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HPM45631.1"
                     /translation="HVALGATMGDFGGWNMPLWYKTGQIAEHKATRENCGLFDICHMG
                     EFIVTGRDSFAFWQKVLTNNVANIVDGQAQYNFMLNESGGVIDDCIVYRFNEEKWMLV
                     VNAGTQLGDYEWLKKNAAGDVKIENISDNTGKLDLQGPTAPKLIAELAGKESIEELKF
                     FRFADNVMIGGVKVLVSRTGYTGEIGFELYCDINETAKLWDILMEKGAKYGITPAGLG
                     ARDTLRLEAGLPLSGSEVGPDVPAVGTPWEFAISYDHEFIGKAALEANKGKLFVYAIE
                     IEGRRKPGHHSKVVLNGTEIGDVTSEILAVTLGMKPAGFIRVNKELGMDTEISVVNPK
                     GQSFKAFVKENPMVKGTSRKKMSSML"
     gene            1114..1515
                     /gene="gcvH"
                     /locus_tag="PL056_01055"
     CDS             1114..1515
                     /gene="gcvH"
                     /locus_tag="PL056_01055"
                     /inference="COORDINATES: protein motif:HMM:NF002270.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HPM45632.1"
                     /translation="MANYNVPSELRYSENHEWVRDMGGNRYRTGITDYAAQHIGDVTY
                     VELPEVGSEVDKESVDCTIETVKSSEDIFNQIAGHIAVVNDALQETPEIINSDCYGDG
                     WLYDIKADSDDDFTSLMTAEEYEKFLSEQEN"
     gene            1592..2950
                     /gene="gcvPA"
                     /locus_tag="PL056_01060"
     CDS             1592..2950
                     /gene="gcvPA"
                     /locus_tag="PL056_01060"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HPM45633.1"
                     /translation="MRYLPITDKERKEMLDRIGAKSVADLFSPLPKNVLLKKELDLPA
                     AKSEMEIEKHMAELGTMNKGSEMLSFLGGGCYRHYSPAIIDQLLLRSEFYTAYTPYQP
                     EVSQGTLQVIFEFQTMVSNLFKMDAANASMYDGATAAAEAVMMASKISKKGTKVLIAS
                     TMHPHYIQTVKTYIEFLVDKVDMLQMNASTGRIDDNELEKIDAETICVLFQYPNYFGV
                     IEDLHKIAAKTKEAGTLLIPVVAEATSLGILATPGDLDADIAVGEGQALGIPLNFGGP
                     GVGLFTVKEKHVRKMPGRLVGQTVDRNGNKCYVLTLAAREQHIKREKATSNICSNQGL
                     MATAAAMYLATMGKSGLREVALMNLNRCEYLKKKVAEETNCKIAFSGHTYNEFTILVP
                     GTGAETLKKLESFGILGGIPVAKYHPELENAIIVNTTEIHSYEELDRFVSALKKVTGG
                     RS"
     gene            2947..4419
                     /gene="gcvPB"
                     /locus_tag="PL056_01065"
     CDS             2947..4419
                     /gene="gcvPB"
                     /locus_tag="PL056_01065"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HPM45634.1"
                     /translation="MSYPGMRDLKLKEETLFERSRQGRCGYSLPKEKLADASSFIPQE
                     LLRKELDLPELSEVDIARHYTRLSTYNFSIDLGFYPLGSCTMKYNPKINEKMARIPAF
                     ANLHPETPVEYSQGTLKLMYEMQKMLSEIGGFAATTLNPSAGAHGEYVGLGIIRSYFK
                     KKGDTKRKKIIIPDSAHGTNPASCTLNGFDVVVLESNESGKVCVDCLKGMLNDEVAGI
                     MLTNPNTLGVFESDIMEITKAVHDAGGLVYGDGANMNAILGIARPGDMGIDVLHYNLH
                     KTFSTPHGGGGPGSGPVGVCEKLAEFLPDPHVVLKDGRFSFANSASSMGMIRSFYGNM
                     AITLRAYVYILEYGKDYIADIAKNAVLNANYIKACLKDYYHLKYKSDTLHEVVFDDSE
                     MPNHVTTMDIAKALIDRGYHPPTVYFPLIVHGAIMIEPTETESKETLDSFIEAMIDIF
                     ETAKTNPDNILKAPLNTAISRPDETIAARKPVLKYCPNAD"
     gene            4427..4786
                     /locus_tag="PL056_01070"
     CDS             4427..4786
                     /locus_tag="PL056_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45635.1"
                     /translation="MEKSKIRITSTKVLCAKCGKEHYVDFVADGHRKYYCDECLKQMH
                     LDRKKGKVKIVFNEKLKKDQYQFICDICGNLRRASYKPKQEKGLIWCKECESKQKDAD
                     RKKKRGNIVIAKKPKDS"
     gene            complement(4773..5438)
                     /locus_tag="PL056_01075"
     CDS             complement(4773..5438)
                     /locus_tag="PL056_01075"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006441052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPM45636.1"
                     /translation="MKIEVIFEDNHLLAVVKPVGIPVQKDNSEKEDLQTILKEYLKKK
                     YGKPGEAYLGIVHRLDQPVGGVMVFAKTSKAAARLSEQIRKNEWRKEYLAIVEGSPRS
                     KKEYIEDYLLKDPESNTVTVVKKDVKGAKLAKLSYEIIEKSGKRALLKIFLETGRSHQ
                     IRVQLASRGFPIVNDQKYSNSRYAKTRGNIALWAASLTIIHPVKGEEMTFFKELPEEF
                     RNL"
     gene            complement(5416..5874)
                     /locus_tag="PL056_01080"
     CDS             complement(5416..5874)
                     /locus_tag="PL056_01080"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HPM45637.1"
                     /translation="METTDTLQLIKYALKLLSQRDHFRSEIISKLKAKKATIAQIEEV
                     IEYLNKFKYINDIKTLSKFAEEISSSGRGVNYFKKKLYDKGALELFSRDVFSVETEIA
                     AAEIFIKKLKTSDGNEILRKLLSRGFSNEAVFLILKKLKKGEVLENRSDF"
     gene            complement(5861..7042)
                     /locus_tag="PL056_01085"
     CDS             complement(5861..7042)
                     /locus_tag="PL056_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HPM45638.1"
                     /translation="MSEHVEKLNELLQLASKVGASDVHLKTYTAPVLRINGVLNKLDE
                     FGVLKPERLNPMIAGILNDEQRKNLKDNNELDLSYSVSGVGRFRVNVFRQRSSLGVVF
                     RTIPFAVKSIDELVLPPIIKKIANYRRGLVLVTGTTGSGKSTTMASMIDYINNSRACN
                     IITIEDPIEFLIRDKKSIISQREVGIDTRSFSTALRGSLRQDPDVIMVGEMRDLETIE
                     IALTAAETGHLVISTLHTLNAAETINRIISVFPSHNQSQIRQQLGSVFAAAISQRLIK
                     RKDERSRVPAIEVLVASELVKEIILDESRFKELHDVIEKGHETYGMQTFDQSIFQLFH
                     DGFITEKEALENATSPDDLILRMQGVSISGDGDWKSFDKEGSGSQYQPTDFDYDPDGN
                     N"
     gene            complement(7042..8106)
                     /gene="recA"
                     /locus_tag="PL056_01090"
     CDS             complement(7042..8106)
                     /gene="recA"
                     /locus_tag="PL056_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HPM45639.1"
                     /translation="MVENKKEMLENVIKAVEKQYGKGSIMILGDENTVDIPVIPSGAL
                     SLDIAMGVGGYPRGRIVEIYGPESSGKTTLALHAIAEAQKKGGAAAFIDAEHAFDINY
                     AKKLGIQVDSLVVSQPDYAEQALEIAETLVRSNAVDIVVVDSVAALVPRSEIEGEMGD
                     SHMGVQARLMSQALRKLTAAVSKSKTCLIFINQLRSKIGVMFGNPETTTGGNALKFYA
                     SMRIDIRKIASIKEKEEVVGNQVRVKIQKNKMFPPFRESVFDIIYGRGIDRLADMVTV
                     AEVDGFINKSGSWYSYNDEKLGQGLSNTVKTLKENPEMIKEIVARIKEKHNLIKDEEP
                     AEKKSAEGEEKSKKKSSGSK"
     gene            complement(8144..8461)
                     /locus_tag="PL056_01095"
     CDS             complement(8144..8461)
                     /locus_tag="PL056_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45640.1"
                     /translation="MRCEKQHTETDFEKLSDSRIILMVVDETLSRNKSYEFFKTKRGQ
                     ELFKKARIVKGFLQDLKNGAKIISENSEKESVLVTLENMDEKYKRTVFMDKNMYSVFS
                     LRK"
     gene            complement(8483..9484)
                     /locus_tag="PL056_01100"
     CDS             complement(8483..9484)
                     /locus_tag="PL056_01100"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HPM45641.1"
                     /translation="MKALILSAGFGTRMSPVTDFIPKPVIPVLNRPVIEYNINFLKHF
                     GIKDLFINLHYGADKISDLLGSGKKFGVKIKYLQESEILGTGGAIASMGPFVSEPFVV
                     INSDTIFDFDLDEILNSHFRSGSKVTLGVVPSSPKDPRAVIRVDENFRIVRMLDASFE
                     KENPEGNAIFTGLHVISPALLEYIPDNIFTSITNYVYKRMVEQKEHINAFFINGKWWD
                     MGTPDDYLECTFDLIKNLPLSYFDPFEKYSLKPEKINPETLVVMGKNIKVPAVPLKPP
                     LIIGNGADLEKVESAGPNLIIGDKVKLSKDSDVSNAVYLNGADGSRALFGKKGKIFY"
     gene            complement(9481..10527)
                     /locus_tag="PL056_01105"
     CDS             complement(9481..10527)
                     /locus_tag="PL056_01105"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HPM45642.1"
                     /translation="MMDMSIEIIAFKMLNIYKSKKIFDFSAEPLFGDASGRQYFRIVF
                     GKESAVLMKVADIKPGEFGRGDSFNDFIAIKNLFDMSGIKVPKIYASIPEQKALLLED
                     LGDTTLFKLIQSEKKNRLKHVRSAVDLLVKTQHHLYNRTFFDSPADKRTFDMKLFMDE
                     FYHFYEYMIAKRVYDKPFNGVWPKLEKKFKQISHDLSTQPYLLSHRDFQSKNIMVKNG
                     VSYLIDFQDALMAPAVYDLVSLLRDSYVVLSDSEVELMLKYYWKTSSTANEIFSCYED
                     LEKAFYLQTVQRKMKDAGRFIYLNQVKGKEWFLPYVVPTLGYVRSTLIKLEMGKIIDV
                     LAPFIPEFNMGDKN"
     gene            complement(10524..11144)
                     /locus_tag="PL056_01110"
     CDS             complement(10524..11144)
                     /locus_tag="PL056_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45643.1"
                     /translation="MSGTGKKTERLDLVGAFEQGWIQYKANFLKIVPYGFLATLPSVL
                     FLFNVTAGVISTVILQGILCILLANSVICSVNGTRSDIYSAPHIWNLFKNGLVLSIFL
                     LPLLAVSFVLLIVPSVIVFSLFMFSFFVVAAKDKFSVDALMDSLRAGFGYRLHLFLFS
                     LIFYCSVIISAIISNFVPFIFILINGLTLPYFFAVIYELYEQLEKK"
     gene            11291..12670
                     /locus_tag="PL056_01115"
     CDS             11291..12670
                     /locus_tag="PL056_01115"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HPM45644.1"
                     /translation="MRTYLTSFIVSFLFVLVTTPFLIKIGIKHGFVDKVDQRKIHQGV
                     IPRIGGIGIAMGTLFPLFLLYFYNNFVSQTLFGSIENVVVIIFGGLSISILGLFDDIK
                     GLPAKFKFLFQIALAVIAIYCGFSINAMSTPWGVVQLGWVGHFITVLWIVGIINAFNL
                     IDGMDGLSSGVSFFACITIMSLSIVNGYMFVALVSAALAGAVVGFLVYNFNPAKIFMG
                     DAGSMFIGYVLAVLSLKNQSKGHTIVSMLVPIIAMGLPILDTTLAFLRRFLRNQSIFS
                     ADKQHIHHILLSKGLNQRKVVFMLYGISVVFTALAMLLIFQKDVEAFLVIVVFSIVVF
                     VIVSKLGYTEMFYSRFKTRKEDRLENHLENYLVEKITIQNYNEMFDVLPISGFEILDC
                     EGKRIALGGTSGGVHFFDIAAEKNKIVRLYWVSSVPTINNREAAMLLVLAKSIVKNSF
                     FSIQENDIK"
     gene            12667..14016
                     /locus_tag="PL056_01120"
     CDS             12667..14016
                     /locus_tag="PL056_01120"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HPM45645.1"
                     /translation="MKVLKFGGISVKDAPAIRKLKEILLLDDSKKLVVVSAFFGVTDL
                     LSELVGQLKTKKYDTASETAGKVEQICLNIAKELRSCKVSERFITEKFCEIKTLIPAL
                     ALLGEITDRSVDMFMSVGEKISSFVLADFLNSEGMNSGFIDASGVIVTDNCHTKAAVD
                     LDKTKAAVSGRIAPLFEHSNIVVTAGFIGSDPTGATTTLGRGGSDYSASVLAVCLKAE
                     VLEIWKEVDGIMTTDPRIVKNAIPVESLSYTEASELAFFGAKVLHPKTIRAAVMEGIP
                     VIVKKTSDPQFQGTTINRTSPVTRKVKAIAFHKNITIINICSEKMLGTYGFLSRVFDI
                     FTKHRTPVDMITTSEINISVTVDSNGPVSDIIRELSEFSSVTATENVSIISIVGEGIK
                     FTSGIAGRCFGSLKDINILMVSMGASEANISIIVDQSDMERCVTLLHREFFEKERKE"
     gene            14013..15239
                     /gene="lysA"
                     /locus_tag="PL056_01125"
     CDS             14013..15239
                     /gene="lysA"
                     /locus_tag="PL056_01125"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HPM45646.1"
                     /translation="MITRQQILKLLETFETPFYLYDGTKIKENCRRITNSFRKYYPST
                     EIHYAVKANSCPGVLNIIKESGLKVDCSSPFELAVAHKTGFEKNDIMYTGNYESTEDL
                     EHALDFAGFINLDDITSFDRLKLIRLPEIISFRINPGIGRGGFEGVVTGGVDAKFGIP
                     YEKALAAYKAAYDSGIKRFGMHMMTGSNNLEPFYFAEITDKLMHIAGTVFGDLKVKPE
                     YIDIGGGFGIPYKNDEPEIDIDSVAKLVTERFIENCKKYGFGTPVLRLEPGRYITGNA
                     GMLITKITGNKDSYRNYVGVDAGMNTLLRPALYDAFHRIEPLVSDGSDTKKTDVCGRI
                     CENSDIFARGVRLPEMKTGDVLIFRDCGAYGYAMSSNYNGRPKAGEIVTTGSKIVYSR
                     PKETLEKMLDYHFKNV"
     gene            15308..15526
                     /locus_tag="PL056_01130"
     CDS             15308..15526
                     /locus_tag="PL056_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPM45647.1"
                     /translation="MVEKTKANKLKDYREERLISKTELARNAEISLVTLDRIEKGFEC
                     RLSTKRKIIEALGLDLDNKDEIFPDDGN"
     gene            complement(15532..16116)
                     /locus_tag="PL056_01135"
     CDS             complement(15532..16116)
                     /locus_tag="PL056_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013006784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylation factor-like protein"
                     /protein_id="HPM45648.1"
                     /translation="MVLINRIMKEITLKIVYYGPGLSGKTTNIEHIYKNITPSNRGKM
                     VSVKTDTERTLFFDFLPVDIGRVKGMNLRTHLYSVAGQVFYDASRRLILKGCDGIIFV
                     VDGQIERLDANIESIENLKKNMLMNSLIFDKVPMVVQYNKTDLPNSLKIEELQKIFNP
                     DGKYSDFNANALSGRNVFETFKTIVRLIIKDMKI"
     gene            16267..17637
                     /gene="purB"
                     /locus_tag="PL056_01140"
     CDS             16267..17637
                     /gene="purB"
                     /locus_tag="PL056_01140"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015904564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HPM45649.1"
                     /translation="MEINNISAVDGRYSGQVKELRDIFSEYGLIKHRVFVETEWLKFI
                     LNGLGFQKLTSEQTASIDSIFSGFNRESALMIKKIESTTNHDVKAVEYYIKEQLENRN
                     MGAVKEWVHFCCTSEDINNCSYALMIKEGKTVVSLTLKELLTSIEALGLKYRSVPMMS
                     RTHGQPASPTTVGKEFINFAVRIQRELKLLEKLEVECKFNGATGNFNAHHFSKPEIDW
                     IAASEKFITEHLVMKPLLWTTQINNYHYISETFHILIRIASTLTDLDRDMWSYVSLGY
                     FKQKTKEGEIGSSTMPHKVNPIDFENSEGNAGIAVSLMEHLSTKLLNSRMQRDLTDST
                     VLRNIGTVFGHILISIKNCLKGLSKIEINEKKIIEDLDDNLELLAEPVQTVMRVYEEE
                     NPYEKLKMLTRGRKIDVESLHKFIDSLEKVPSNIKKEMKDLKPSDYTGIAEKLVDLYF
                     KKRESL"
     gene            17732..18745
                     /locus_tag="PL056_01145"
     CDS             17732..18745
                     /locus_tag="PL056_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45650.1"
                     /translation="MKGYIFFPVLFFLLINISCTSDGLKEDFSDIPDWDYSNVVTDEP
                     SGNQDDNDSEETPDNSNSTDPADENISTGDQEETTDDGGTIQGCGNEKVEYEERCDST
                     VPLTCSSLNPNMSGVTYCMNNCQGYDMQSCGRSDKSWGLLNLRFLSNFILDEAKIGDY
                     SYFSKGALPYAAFNGVYGDTVTIFPTIGENVSFAVTNNYKGTLGGNKRQLTIKQNAVA
                     GGIGYPRHELEFSPGTISKGSEYRINAVTIFDLADNLLKLVRYRLIEKKDGVECIMGI
                     GYSGTVYINNIVPDNADLFDGGIIEVVANNVDFFHPSEFPGLDEENPEVPAEILKYPV
                     CDK"
     gene            18752..20098
                     /gene="mnmE"
                     /locus_tag="PL056_01150"
     CDS             18752..20098
                     /gene="mnmE"
                     /locus_tag="PL056_01150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HPM45651.1"
                     /translation="MYFDRINETICALATSSGSSIGIIRISGKNSFKIASSLCGIKSF
                     EHMRAKIVKIILQGKTLEKCLLLPFKAPKSFTGEDVVEFHIHGSNKNGEEIMSLLLEK
                     GAVPALRGEFSFRAVMNSKMELSQAFALNTLITSSNPVSVELSRKAAFEDRALKKLFL
                     LLNEWEHFFVLTTAIIDFPDQVENELPAHKLSELAEKTEKYLSETLDNSALLEKTETF
                     SVLIAGKPNVGKSSLFNILINKDRAIVSHDAGTTRDYITDSLNINGIGVQVIDSAGLR
                     EGLSETEQQGIDKSRRLIKHADLLLLMFDGSLPIEEKDLELLTETSIYPRILIINKID
                     GHQPEVFPEEFKNALKISCLEEKGINAVIEAIQEQIKKTMPDKNKDLFFSSWQKDLTV
                     DTLEQISELETYIISGEIEIINVLIKKIFENLKDLTGTISSHSVYDRIFSGFCLGK"
     gene            20245..22113
                     /gene="mnmG"
                     /locus_tag="PL056_01155"
     CDS             20245..22113
                     /gene="mnmG"
                     /locus_tag="PL056_01155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HPM45652.1"
                     /translation="MDYFDVAVIGGGHAGIEASYASSKTGASTLLITTSIEKIGEMSC
                     NPSIGGQAKGQIVREIDIFGGLMGRAADYSAIQYRILNRRKGTAVQALRAQCDKNLYK
                     EFVRNFLFQTPNLKIFQGEAFSLEHGENSLFKITTSGGSFFQSKAVIVTAGTFLNGTV
                     HIGLNNFSSGRLGEPASSGLSEYISGFGHKKVRLKTGTPVRILGNSIDFSKMETQEGE
                     TDIIPFSIFSGSVSKRQIPCYLTRTTDLTKKIILENLHFSPMYGTSKSIEGIGPRYCP
                     SIEDKFVKFPLKESHQIFVEPEGWDCFEYYPNGISTSLPFNVQKEFLKTIPGLENAVI
                     TRPAYAIEYDSFDPTDIDLTLMSRFVPGLFLAGQVNGTSGYEEAAGQGLVAGVNAAYF
                     SKKNGKIFVPGRSKSYIGVMISDITSKGVDEPYRMFTSRAEYRLSIRDNNVAERLLEL
                     SFSNGLISEKVFLEEKRKCSLVSELVTMLNEKIIYPDKETNARMEKLSETPLNKPVSL
                     STVLKRPSVGRKELFLISGYVSDAGNEIWSRAETEIKYSSFVEREKAEIQKFEELSSI
                     SIPDGFDFNSISGFTNEIKQKLSARRPKDLAQASMIPGITPAAISILIMHLKRDKA"
     gene            22110..>22700
                     /gene="rsmG"
                     /locus_tag="PL056_01160"
     CDS             22110..>22700
                     /gene="rsmG"
                     /locus_tag="PL056_01160"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HPM45653.1"
                     /translation="MTEKFPESCVSCETFHRKINLLLNPYDVSIDIESAEKLVSFWNF
                     FSHWNKAHNLSSVTDLEEATFIHFADSLFPASEKEIFLKEKRVLDFGTGGGFPGIPLS
                     LYFKDCQFFLLDKCRKKTSFISFAAASLGIKNVFPVNALLEKHKGTKYDIITSRAVKI
                     DKKMFDLCRSILNEDGWLIIYYSLSQKPVESEFLSHV"
BASE COUNT         6850 a         4814 c         4637 g         6399 t
ORIGIN      
        1 agcatgtggc actcggtgct acaatgggtg atttcggcgg atggaacatg ccgctctggt
       61 acaaaaccgg tcagatagct gaacacaagg cgacaaggga aaactgcggt ctttttgata
      121 tctgccacat gggtgaattc atcgtgacag gtcgtgattc gttcgctttc tggcagaaag
      181 tgctaacaaa caatgttgca aacatcgttg acggacaggc acagtacaat ttcatgctca
      241 atgaatcagg cggcgtgatc gatgactgca tcgtttaccg cttcaatgaa gaaaaatgga
      301 tgcttgtcgt aaacgcaggc acacagcttg gcgactatga atggcttaaa aagaacgcgg
      361 caggcgatgt aaaaatcgaa aacatcagcg acaacaccgg aaagcttgac cttcagggtc
      421 cgaccgctcc gaaacttatc gctgaacttg ccggaaagga atccattgaa gagcttaaat
      481 tcttccgttt tgcagacaat gtcatgatcg gaggagttaa agtccttgtc agcagaaccg
      541 gatacaccgg agagattggc tttgaacttt actgcgatat aaacgaaaca gcaaaactct
      601 gggacatttt gatggaaaaa ggggcaaaat acggaatcac accggcaggg ctgggagcaa
      661 gagacactct tcgtctggaa gcaggccttc ccctttccgg cagtgaagtc ggacctgatg
      721 ttccggcagt gggaacaccg tgggaatttg caatttcata cgatcacgag ttcatcggaa
      781 aagcggctct tgaagcaaac aaaggaaaat tgttcgttta tgcgatagaa attgaaggaa
      841 gaagaaaacc gggacatcac tccaaagttg ttctcaacgg aacagagatc ggagatgtca
      901 caagcgagat ccttgctgtg actctgggaa tgaaaccggc aggattcatc cgtgtaaata
      961 aagagctcgg aatggatacg gaaatatcgg tcgtcaatcc caaaggccag tcatttaaag
     1021 catttgttaa agaaaatcca atggttaaag gaaccagcag aaagaaaatg tcatcaatgt
     1081 tataacactt ttacttaagt atggaggaga aaaatggcta attacaatgt accgagcgaa
     1141 ctcaggtatt ctgaaaatca cgaatgggta agagacatgg gcggaaacag ataccgcaca
     1201 gggattacag attatgcggc tcagcatata ggcgatgtca catatgtcga acttcccgaa
     1261 gtgggttcag aagttgataa ggaatctgtt gactgcacaa ttgaaacagt gaaaagttct
     1321 gaagacatct tcaatcagat agccggtcat attgccgttg tcaatgatgc tcttcaggaa
     1381 accccggaaa taattaattc cgactgttac ggtgacggct ggctttatga catcaaagct
     1441 gacagcgatg acgactttac ttcgctcatg acagctgaag aatatgagaa atttctttca
     1501 gagcaggaaa actgatcttg tccaaaactt tgaatgaata ccggggagcg ataagtcgtt
     1561 cccctttttt tatgaacttt tggaggtaaa aatgagatac cttcccatta ccgataaaga
     1621 aaggaaagag atgcttgaca ggatcggtgc aaaaagcgtt gccgaccttt tttcacctct
     1681 tccgaaaaat gttcttctta aaaaagagct cgatctccct gccgcaaaaa gcgaaatgga
     1741 gatagaaaaa cacatggctg agcttggaac aatgaacaag ggctcggaaa tgctctcttt
     1801 tctgggcggc ggatgttatc gccactacag ccctgccata atcgaccagc tgcttttaag
     1861 aagtgaattt tacacagctt ataccccgta tcagcccgaa gtgagccagg gaacgctcca
     1921 ggtcatattt gaatttcaga caatggtttc caaccttttt aaaatggatg ccgcaaacgc
     1981 ttcgatgtat gacggtgcaa cagccgccgc agaagctgta atgatggctt caaagatctc
     2041 taaaaaaggg acaaaagtgc tgatcgcttc gacaatgcac ccccactaca tccagactgt
     2101 aaaaacatat attgagtttc tcgttgacaa agtcgatatg ctccagatga acgcctcaac
     2161 cggtcgtatt gacgataatg agcttgaaaa aatagatgcc gaaacaattt gtgttctgtt
     2221 ccagtatcct aattatttcg gtgtgataga agatcttcac aagatcgcgg caaaaacgaa
     2281 ggaagcggga acacttctta ttcctgtcgt tgctgaagct acatctcttg ggatccttgc
     2341 aactcccggc gaccttgatg cggacattgc agtcggagaa ggacaggctc tcggaattcc
     2401 tttgaatttc ggcggacccg gtgtgggact tttcacagtt aaagaaaaac atgtaagaaa
     2461 aatgcccgga cggcttgtcg gccagactgt cgacagaaac gggaataaat gttatgtcct
     2521 cacccttgcg gcaagagagc agcatataaa aagagaaaaa gcgacttcaa acatctgctc
     2581 caatcagggt ctgatggcta ctgcggccgc aatgtacctt gcaacgatgg gaaaaagcgg
     2641 acttagagaa gttgctttga tgaacctcaa ccgctgcgaa tatttaaaga aaaaggtcgc
     2701 tgaagaaaca aattgcaaaa tagctttttc aggacacacc tacaacgaat ttaccatact
     2761 tgttcccgga acaggggcgg aaacactcaa aaaacttgaa tctttcggca tacttggagg
     2821 aattcctgtt gcgaaatacc atcccgagct tgaaaatgcg ataattgtaa acacaacaga
     2881 aattcactct tatgaggaac ttgacagatt tgtgtcggct ctgaaaaaag ttacaggagg
     2941 tagatcatga gttacccagg aatgagagat ctcaaactta aagaggagac cctttttgaa
     3001 agaagccgtc agggaagatg cggatattct ctccccaaag agaaacttgc cgatgcctct
     3061 tctttcatac ctcaggaatt attgagaaaa gagctcgatc tgcctgaact tagcgaagtt
     3121 gacatagcaa ggcattacac ccgtctttca acttataatt tcagcatcga tcttggtttt
     3181 tatccgcttg gaagctgtac aatgaaatat aatcccaaga taaatgaaaa aatggcccgc
     3241 attcccgctt ttgcaaatct tcatcctgaa acacctgtcg aatattctca gggaacgctg
     3301 aaactgatgt atgaaatgca gaaaatgctc agtgagatcg gagggtttgc cgcaacaacg
     3361 cttaacccgt ctgccggagc tcacggagag tatgtcggcc ttgggatcat aagaagttat
     3421 ttcaagaaaa aaggcgacac aaaaagaaaa aaaataataa ttcccgacag tgcacacgga
     3481 acaaatccgg caagctgcac ccttaacggt tttgatgtag tagtccttga aagcaatgaa
     3541 tcgggaaaag tctgtgttga ctgtctcaag ggaatgctta atgacgaagt tgctggcata
     3601 atgctcacaa atccgaacac tcttggtgtt tttgagagcg atatcatgga gataaccaaa
     3661 gccgtgcatg atgccggcgg actggtttac ggtgacggag cgaacatgaa tgcgatactc
     3721 ggcattgcaa ggccgggcga catgggaata gatgttcttc attacaatct tcacaaaacc
     3781 ttctcaacac ctcacggcgg cggcggtccc ggatcaggcc cggtaggtgt gtgtgaaaag
     3841 cttgctgaat tccttcccga cccgcatgtt gttcttaaag atggcagatt ttcgtttgca
     3901 aattcagcca gttcaatggg aatgatcagg tcattttatg gaaatatggc aataacttta
     3961 agggcttatg tctatattct tgaatatggc aaagactata tcgccgacat tgcaaaaaat
     4021 gcggttctta acgcgaatta tatcaaagcg tgccttaaag actactacca tctcaaatat
     4081 aaatcagaca ctcttcatga agttgttttt gatgattctg aaatgcccaa tcatgttaca
     4141 accatggata tcgcaaaagc gctcattgat cgcggatatc atccaccgac agtctatttc
     4201 ccccttatcg ttcatggtgc gataatgatc gagccgactg aaactgaaag caaggagacg
     4261 cttgattctt tcatcgaggc aatgatcgac atatttgaaa ctgctaaaac caaccctgac
     4321 aatattctca aagcaccgct taacactgcc ataagccgtc cggatgaaac gattgcggca
     4381 agaaaaccgg ttctcaaata ctgtcccaac gctgattgag gtaaatttgg aaaaatcgaa
     4441 aataaggatc acatcaacaa aagtcttatg tgccaaatgc ggaaaagaac attatgtcga
     4501 ttttgtagca gacggtcaca gaaaatatta ctgcgatgaa tgtctcaaac agatgcatct
     4561 tgacagaaag aaagggaaag tcaaaatagt tttcaacgaa aaactgaaaa aagaccagta
     4621 ccagttcatc tgtgatatct gcggaaatct cagaagggct tcatacaaac ccaaacagga
     4681 aaaagggctg atctggtgca aggaatgtga aagcaaacag aaagatgcag accggaaaaa
     4741 gaaacgcggt aatattgtta tcgcaaaaaa acctaaagat tcctgaactc ttccggaagc
     4801 tccttaaaaa atgtcatctc ttcccctttg acagggtgga ttatcgttaa agatgccgcc
     4861 cagagtgcta tgtttcctct ggtttttgca tatctgctgt tgctgtattt ttggtcgtta
     4921 actatcggaa atccgcgtga ggcaagctga actcttatct ggtgagacct tcctgtttca
     4981 agaaagatct taagaagtgc ccttttcccg cttttctcaa tgatttcata acttaatttg
     5041 gcgagttttg cccctttgac atcctttttt acaacagtta ctgtgttgct ttcggggtct
     5101 tttaaaagat agtcttcaat atattccttt tttgatcttg gagacccttc aacaattgcc
     5161 agatactcct ttctccactc gttctttctt atctgttcac tcaggcgggc ggcagctttt
     5221 gatgtttttg caaaaaccat tacccctccg accggctgat caagacggtg aactattcca
     5281 agatatgctt cacccggttt tccatacttc tttttgagat attcttttaa gatcgtctga
     5341 agatcttctt tttcagagtt atctttctgg accggaattc caactggttt tacaactgca
     5401 agaagatggt tatcttcaaa aatcacttct attttcaagc acttctcctt ttttaagttt
     5461 ttttaaaatt aaaaacaccg cttcatttga aaaacctctt gagagaagtt ttcttaaaat
     5521 ttcattcccg tctgaagttt tcagtttttt aatgaaaatt tctgcagcgg caatttcagt
     5581 ctccacagag aaaacatctc ttgaaaaaag ctcaagcgcc cctttatcat aaagcttttt
     5641 tttgaaatag ttcacccccc ttcccgatga tgagatttcc tcagcaaatt tactcaaagt
     5701 ttttatatcg tttatatatt tgaatttatt aagatattca atcacttcct cgatctgtgc
     5761 gatagtcgct ttttttgctt ttaattttga tatgatctca gatctgaaat ggtctctctg
     5821 agaaagcagt ttcagagcat attttatgag ttgtaaagta tcagttgttt ccatcagggt
     5881 cgtaatcaaa atctgtgggc tgatactggc tgccgcttcc ctctttgtca aaagatttcc
     5941 agtcaccgtc tcctgaaatg cttactccct gcatcctgag tatgagatca tcgggacttg
     6001 ttgcattttc aagtgcctct ttttctgtga tgaacccgtc atgaaaaagc tggaagatcg
     6061 actgatcaaa agtctgcatt ccgtatgttt cgtgcccttt ctcaattaca tcgtggagct
     6121 ccttgaacct gctttcatca agaattatct cttttacaag ttctgatgcc acaagaactt
     6181 caattgcggg aacccgcgat ctttcgtctt ttctttttat caatctctga cttattgcgg
     6241 cggcaaaaac actgccgagc tgctgacgga tctgggactg gttatgtgac ggaaaaactg
     6301 aaataatacg gttaattgtc tcggcagcgt taagtgtgtg gagagtgctt atcacaagat
     6361 gacctgtttc cgccgcagta agagctatct cgatcgtttc gagatctctc atctctccca
     6421 ccatgatgac atccgggtcc tgccttaacg aacctctgag tgctgtggag aacgatcttg
     6481 tgtctatccc gacttccctc tggctgatga tgcttttctt gtcacggata aggaactcta
     6541 tcgggtcctc gatcgtaata atgttgcagg cccttgaatt gtttatgtaa tctatcatcg
     6601 aagccatggt cgtagatttt ccgcttccgg tcgttccggt aacaaggaca agtcctcttc
     6661 tgtaatttgc tatctttttt atgatcgggg gaagcacaag ttcatcaatt gatttaaccg
     6721 caaaaggaat cgtcctgaaa acaacaccga gcgaacttct ctgacggaaa acattcaccc
     6781 tgaacctgcc aactccggaa acactgtatg aaaggtccag ttcgttgtta tccttgaggt
     6841 tttttctctg ttcatcattc agaatgccgg ctatcatcgg attaagccgt tcgggcttga
     6901 gcactccgaa ctcgtcaagt ttgttaagaa cgccgttgat ccgcaaaacc ggagcggtat
     6961 aggttttcag atgaacatcg ctggcaccga cttttgatgc caactgtaga agttcattca
     7021 gtttttcgac atgttcactc atcatttact cccggaactt ttcttttttg atttttcttc
     7081 accttccgca ctcttttttt ctgccggttc ctcgtctttt ataagattgt gcttttcttt
     7141 tatccttgca acaatttcct taatcatttc agggttttcc tttaaagttt taaccgtatt
     7201 gctaagtccc tgtccgagtt tttcatcatt atagctgtac cagctcccgc ttttgttaat
     7261 gaatccgtca acttccgcaa cagttaccat gtctgcaaga cgatcgatcc ctcttccgta
     7321 aattatatca aaaacagatt ctctgaacgg agggaacatt ttatttttct gtattttaac
     7381 tctcacctga ttgccgacaa cttcctcttt ttcctttatg cttgcgatct tccttatgtc
     7441 aattctcatg gaagcataaa atttcaaagc attcccgcct gttgttgttt caggatttcc
     7501 aaacatcaca ccgatcttgc ttctgagctg gttgataaaa ataagacaag tctttgattt
     7561 gctcacagcg gcggtaagtt tacggagtgc ctgcgacata agccgtgcct gaacacccat
     7621 gtgactgtcc cccatctccc cttctatttc gcttctcggg acaagggcgg caacagagtc
     7681 cacaacaacg atgtcaactg cattgcttct gacaagggtt tccgctattt caagtgcctg
     7741 ttcggcataa tcgggctgtg aaacaaccag tgaatcaacc tgaatgccga gttttttcgc
     7801 atagttgatg tcaaaagcgt gctcggcgtc aatgaaagcg gcggcgccac ccttcttctg
     7861 tgcctcggcg atcgcatgga gggcaagtgt tgtctttcct gaagattcgg gaccgtagat
     7921 ctcgactatt cttcccctcg ggtaaccgcc aacacccatt gcaatgtcaa gactcagcgc
     7981 tcccgacggg atgacaggga tgtcgactgt gttttcgtcc cccagtatca ttattgatcc
     8041 ttttccatac tgtttttcta cagctttgat cacattttca agcatttcct ttttgttttc
     8101 gaccatttta ctctcctacg gtttgattca gattgttaaa cagctatttt ctcaagctga
     8161 atacagaata catattttta tccataaata cagttctttt atatttttca tccatatttt
     8221 ccagagtgac aagaacgctc tctttttcac tgttctccga aattattttt gcaccgtttt
     8281 tcagatcctg gagaaaacct tttacaattc tcgccttttt aaaaagctcc tgcccccttt
     8341 ttgttttgaa aaactcatag cttttattcc ttgacagggt ttcatccaca accataagga
     8401 tgatccttga gtcactcaat ttttcaaagt cggtttctgt atgctgcttt tcacatctca
     8461 tgagaccgcc tccccgaaaa gatcagtaaa atattttccc ttttttgcca aacagcgccc
     8521 ggcttccatc cgctccgtta agatacacag cattagaaac atctgagtct ttcgaaagct
     8581 tcaccttgtc tcctattata agattcggcc ccgccgattc cactttttca agatccgccc
     8641 cgttgcctat tatcaaaggg ggcttgaggg gaactgccgg aactttgatg ttctttccca
     8701 tcacaacaag tgtttccgga tttatttttt ccggttttaa agaatatttc tcaaaaggat
     8761 caaagtatga aagaggaaga ttcttgatta agtcgaatgt gcactcaaga taatcgtcgg
     8821 gagttcccat atcccaccat tttccattta taaaaaaggc atttatgtgt tctttctgct
     8881 caaccatcct tttgtaaaca tagttcgtta tcgatgtgaa aatattatca ggaatatatt
     8941 caagaagggc gggacttatg acatgcagcc ctgtaaatat cgcatttcct tcaggatttt
     9001 ccttttcaaa agatgcgtcc agcatcctta caatcctgaa attctcgtca accctgataa
     9061 cagctctggg gtctttaggg gaggacggga caacacccag agtaaccttg cttcctgatc
     9121 tgaaatggct gttaagtatt tcgtcaagat caaaatcaaa gatagtgtct gaatttatca
     9181 caacaaacgg ctcactcaca aaaggaccca tgcttgcaat ggcccctcct gtaccaagta
     9241 tttcgctttc ctggagatat tttatcttta ccccgaactt cttgccgctc ccgaggagat
     9301 cagatatctt gtcagcaccg taatgaagat ttataaaaag gtcttttatg ccgaaatgtt
     9361 taagaaaatt aatattatat tcaatgacag gcctgttaag tacaggtatc accggctttg
     9421 gaatgaaatc tgtcaccgga gacattcttg ttccaaaacc cgcagaaagt attagtgctt
     9481 tcaattttta tcccccatgt taaattcggg aatgaacggc gcaaggacat cgatgatctt
     9541 tcccatttca agttttatca gtgtgcttct gacatatccc agagtcggaa ccacatacgg
     9601 aaggaaccac tctttgccct taacctgatt aaggtatatg aatcttccgg catctttcat
     9661 tttgcgctga accgtctgaa gatagaatgc cttttcaaga tcttcataac aggaaaagat
     9721 ctcgtttgcc gtactgcttg ttttccagta atatttcaac atcaattcaa cttcactgtc
     9781 agaaaggacc acatatgagt cgcgaagcaa tgataccaga tcataaacag caggcgccat
     9841 caatgcatcc tgaaaatcta tcaggtagct gacaccgttt ttcaccatta tgttctttga
     9901 ctgaaaatcc ctgtgtgaga gaagataggg ctgggttgag agatcgtgcg atatctgttt
     9961 aaacttcttt tccagtttag gccagacacc attgaagggt ttgtcataga ccctttttgc
    10021 gatcatatat tcgtaaaaat gataaaattc gtccataaac agcttcatgt caaaagttct
    10081 tttatcggca ggcgaatcaa aaaaagtcct gttgtaaaga tggtgctgtg tttttacaag
    10141 aaggtcaaca gcgctcctca catgcttcag ccggttcttt ttttcactct ggatcagctt
    10201 gaaaagtgtc gtatcaccga gatcctcaag aaggagggct ttctgttcag gaatggatgc
    10261 ataaatcttc ggaactttta tccctgacat gtcaaaaaga ttttttattg ctatgaaatc
    10321 gttaaaagaa tctcctcttc cgaattctcc cggctttatg tcggcaacct tcatcagaac
    10381 tgcggattct ttcccgaaaa ctatcctgaa atattgtctc cccgaagcat caccaaaaag
    10441 tggctcagca gaaaaatcaa atattttctt tgacttatag atgttcagca tcttgaatgc
    10501 aatgatctct atggacatat ccatcatttt ttctccaact gttcgtaaag ttcataaatt
    10561 acagcaaaaa aataaggcag ggtcaaaccg tttatcaaaa tgaatatgaa cggcacaaaa
    10621 tttgaaataa tggcactgat gatgaccgaa cagtaaaaga tcaaagaaaa aaggaacagg
    10681 tgaagccggt atccgaaccc tgccctgagc gaatccatca atgcatcaac agaaaacttg
    10741 tcctttgcgg caacaacaaa aaaggagaac atgaaaagac tgaaaactat aaccgacgga
    10801 actatcaaaa gaacaaagct aaccgcaagc agaggaagaa gaaaaattga aaggacaagt
    10861 ccgtttttaa acaaattcca gatatgcgga gcggaataga tgtcgcttct tgtcccgttt
    10921 actgaacaga tcactgaatt tgcaagcagt atgcagagaa ttccctgaag aataacagtt
    10981 gatatcaccc ctgcagtaac attaaaaagg aaaagaactg aaggaagtgt cgcaagaaaa
    11041 ccataaggaa cgatctttaa aaaatttgcc ttgtactgta tccacccctg ctcaaaagcc
    11101 cccacaaggt caagccgttc agtttttttt cccgttccgc tcattttttc ctcctcaata
    11161 agctttaaca tattttatct acaaacacca agcggtcaat attattattg tatagatgct
    11221 ctctttaatt ttttattgat ttcacttcga tttgcaataa catagttacc gaatttttga
    11281 aggtattttt atgaggactt atctaacatc tttcattgtt tcatttcttt ttgtgcttgt
    11341 gacaaccccc tttctcataa agatagggat taagcacggt tttgtagaca aagtagacca
    11401 gcggaagatc catcagggtg tcattcccag aataggcgga ataggaattg ccatgggaac
    11461 gcttttcccc ctttttctgc tttattttta caacaatttt gtttcgcaga ccctttttgg
    11521 atcgatagaa aatgttgttg tcattatttt cggagggctc tcaataagca ttctcggtct
    11581 ttttgacgac ataaaagggc tccctgcaaa attcaagttc cttttccaga tagcgcttgc
    11641 ggttattgca atttactgcg gattttcaat aaatgccatg tccacgccgt ggggtgttgt
    11701 tcagctggga tgggtgggac actttataac tgtactctgg atcgtcggaa taataaacgc
    11761 attcaacctg attgacggga tggacggcct ttcttcggga gtatcttttt ttgcatgcat
    11821 aaccataatg tccctttcca ttgtcaacgg atatatgttc gtcgctcttg tttcagccgc
    11881 actcgccggt gctgttgtcg gttttctcgt ttataatttc aaccctgcaa aaatatttat
    11941 gggtgatgca ggatcgatgt tcatcggata tgttcttgcc gttctctctc ttaaaaacca
    12001 gtcaaaggga cacaccatcg tttcaatgct cgttcccata attgccatgg gtctgcccat
    12061 acttgacaca acccttgcat ttttaagaag atttttaaga aatcagtcta ttttttcagc
    12121 cgacaagcag catattcatc acatacttct ttcaaaaggg ctcaaccaga gaaaggtcgt
    12181 gtttatgctt tacggtattt ctgtcgtttt cacagcactt gcaatgcttc tgatctttca
    12241 gaaggatgtt gaggcatttc ttgtaattgt ggttttctcc atcgtcgttt ttgtcattgt
    12301 gagcaagctc ggttatactg aaatgttcta cagccggttc aagacccgga aagaagaccg
    12361 ccttgaaaat catcttgaaa actaccttgt tgaaaagata accattcaaa attacaacga
    12421 aatgtttgat gttcttccaa tctcaggatt tgaaatcctt gactgtgaag gcaaaaggat
    12481 agctctgggg ggaacatccg gcggcgtcca tttttttgat atagcggcag aaaagaataa
    12541 aattgtaaga ctttactggg tctcttcggt cccgaccata aacaatcgtg aagccgcaat
    12601 gcttcttgtt cttgcaaaat caatcgtgaa aaattcattc ttttccattc aggagaacga
    12661 tattaaatga aagttcttaa attcggtggc atttcggtca aagatgcccc ggcgatcaga
    12721 aaattaaaag agatccttct gctcgatgat tcaaaaaaac tcgtggtcgt atccgctttt
    12781 ttcggagtta cagaccttct ttccgaactt gtgggtcaac tcaaaacaaa aaaatacgac
    12841 acagcatcag aaacagccgg aaaggttgag cagatctgcc tgaacattgc taaagagctc
    12901 cggtcatgca aagtttcaga aaggttcatc acagaaaagt tctgcgaaat aaagacactg
    12961 atccccgccc tggcgcttct tggtgaaata acagaccggt cggttgacat gttcatgtct
    13021 gtcggagaaa agatcagcag ctttgtcctt gcggatttcc ttaactcgga aggaatgaat
    13081 tctggtttta tcgatgcatc cggcgttatt gtaactgaca actgccacac aaaagcagcg
    13141 gtagatcttg ataaaaccaa agcagcggtc tccggcagaa tagctcccct ttttgaacac
    13201 agcaatatag ttgtaaccgc cggtttcatc gggtcggacc caactggtgc aacaacaact
    13261 ctcggaagag ggggaagcga ctattcagca tctgttcttg ccgtatgcct gaaagcggaa
    13321 gtccttgaaa tatggaaaga agttgacggt ataatgacaa cagaccccag aatagtgaaa
    13381 aatgccatcc ctgtggaatc gctttcctat accgaagctt ccgagcttgc atttttcgga
    13441 gcaaaagtgc ttcatcccaa aacgataaga gcggccgtaa tggaagggat ccctgtgatc
    13501 gttaaaaaaa ccagcgaccc tcagtttcaa ggaacgacaa taaacagaac ttcgcctgtc
    13561 acaagaaaag tcaaagccat cgcttttcac aaaaacataa caataataaa catctgctct
    13621 gaaaaaatgc tcggaacata cgggttctta agcagagttt tcgatatttt cactaaacac
    13681 agaacaccgg ttgacatgat aacaacatcg gaaataaata tcagcgtcac ggtggattca
    13741 aacgggcctg tttcagacat catcagggag ctttcagagt tctcgtcggt aaccgcaaca
    13801 gaaaatgtca gtattatcag catcgtaggt gaaggcatta agtttacatc cggaattgcc
    13861 ggaagatgtt tcggttcttt aaaggatata aacattctta tggtaagcat gggtgcttca
    13921 gaagccaaca tcagcataat cgttgatcaa tcggacatgg agcggtgtgt aactcttctt
    13981 cacagggaat tttttgaaaa agagaggaaa gaatgatcac aaggcaacaa attttaaagc
    14041 tgcttgaaac ttttgaaacc cctttctatc tttatgacgg aacaaaaatc aaagaaaact
    14101 gccgcaggat cacgaactct ttcagaaaat attatccgtc aacagagata cactatgccg
    14161 taaaagccaa cagctgtccg ggagtgctta atattattaa ggaatcagga ctgaaagttg
    14221 actgttcgag tccttttgaa cttgctgtcg cccacaaaac cggctttgaa aagaacgata
    14281 tcatgtacac cggaaattat gaaagcactg aagaccttga gcatgctctt gattttgccg
    14341 ggtttatcaa tcttgacgac ataacatcct ttgaccggct taagctcatc cgactgcctg
    14401 agatcatctc tttcagaata aaccccggca tcggaagagg cggatttgaa ggggttgtaa
    14461 cgggcggagt cgatgcaaag ttcgggatac cttacgaaaa agcacttgcc gcatataagg
    14521 cggcttatga cagcggaatt aaaagattcg gaatgcacat gatgactggt tcaaacaatc
    14581 tggaaccgtt ctacttcgct gaaattactg acaaacttat gcatattgcc ggaactgttt
    14641 tcggcgatct caaagttaaa cccgaataca tcgacatagg cggcggattc ggaattccgt
    14701 acaagaacga cgagccggaa atagacatcg attccgttgc caagcttgtg accgaaagat
    14761 tcattgaaaa ctgtaaaaaa tacgggttcg gaacccccgt cctccggctg gaaccgggaa
    14821 gatacattac agggaatgcg ggaatgctta taacaaaaat aacaggaaat aaagatagtt
    14881 atagaaatta tgtcggggtt gatgccggca tgaacactct tttaagaccc gcactttatg
    14941 acgcattcca cagaatagag ccactcgttt ctgacggttc cgatacaaaa aaaacagatg
    15001 tgtgcggcag gatctgtgaa aattcagata tttttgcaag aggagtccgc cttccggaaa
    15061 tgaaaacagg agatgttctc atattcaggg attgcggagc atacggatat gcaatgagct
    15121 caaattataa tggcagaccc aaagccggag aaattgttac aacgggatca aagatcgttt
    15181 attccagacc gaaggaaacc cttgaaaaaa tgctcgatta ccattttaag aatgtttgag
    15241 ggggcaaaac ccttgactta tgtttaataa ccgttagaat ctttccgtta ttgaatgagg
    15301 agctgacatg gttgaaaaaa caaaggcgaa caagctgaaa gattataggg aagagcgcct
    15361 tataagtaaa actgaacttg cgagaaacgc ggaaatatca cttgtaacac tggatcgcat
    15421 cgaaaaaggt tttgaatgcc gtctctcaac aaaaagaaaa atcattgagg ccctcgggct
    15481 tgatcttgac aacaaggatg agatatttcc tgatgacggg aattgatctg atcagatctt
    15541 catgtcttta attataagcc gcactatcgt tttaaatgtt tcaaacacat ttcttccgct
    15601 aagagcattt gcgttgaaat ctgaatattt tccatcagga ttgaatattt tctgaagttc
    15661 ctctattttc aaagagttag gaagatctgt cttattatat tgcacaacca tgggaacttt
    15721 gtcgaatatt aaagagttca taagcatgtt ctttttaagg ttttcgatgc tttcaatgtt
    15781 ggcatcaagg cgctcaattt gtccgtcaac aacaaaaatt ataccgtcac atccttttaa
    15841 tatgagtcgt ctggaagcat cataaaaaac ctgcccggca acagaataaa gatgagttct
    15901 gaggttcatc cccttcaccc tgcctatatc aaccggaaga aaatcaaaga acagcgttct
    15961 ttcggtatct gtcttgacag aaaccatttt ccctctgttt gagggggtta tgtttttata
    16021 aatatgctct atgtttgttg tttttccgga aagaccgggt ccgtaataaa cgattttaag
    16081 tgtaatctct ttcataatcc tgtttataag aaccatgtgg cgcaacctct ttcaggtatc
    16141 tcaaaatttt tgacagggaa tgatacttat atgcagatcg aaaaacaaat ttttacataa
    16201 ctttgtagat tttcaattta aactttgtat tatttactgt tttgaactgt ttatggagag
    16261 cagaaaatgg aaataaataa catttccgca gttgacggaa gatacagcgg tcaggttaag
    16321 gagcttcgtg atatattttc agaatacgga ttgataaaac acagagtctt tgtggaaaca
    16381 gagtggctta aatttatttt gaacggactg ggttttcaga agcttacctc agaacagacc
    16441 gcttccattg acagcatctt ctccggattc aacagagaaa gcgccctgat gataaaaaag
    16501 atagaaagta ctacaaatca cgatgtcaaa gccgtggaat actacataaa agaacaactt
    16561 gaaaacagaa acatgggagc tgtcaaggaa tgggttcatt tctgctgtac atctgaagac
    16621 ataaacaact gctcatacgc attgatgata aaagagggaa agaccgttgt ctctttaacc
    16681 ctgaaagagc ttttgacctc aatagaagct ctggggctca aatacagatc cgttcctatg
    16741 atgtcaagaa cacacggtca gcccgcatct cccacaacag tcggcaaaga gttcataaat
    16801 tttgccgtaa gaattcaaag agagctcaaa cttctggaga aattggaagt tgagtgtaaa
    16861 ttcaacggtg caacgggaaa tttcaatgca catcattttt caaaaccgga aattgactgg
    16921 atagctgctt cagaaaaatt cataacggaa caccttgtga tgaaacccct gctctggacg
    16981 acacagataa acaactatca ttatatttca gaaacattcc atatacttat aagaattgcg
    17041 tcaacactta cagaccttga cagagatatg tggagctatg tttctctggg ttattttaaa
    17101 cagaaaacaa aagaggggga gatcggctct tcaacaatgc cgcataaagt aaatccgatt
    17161 gattttgaaa acagtgaagg aaatgcggga attgccgttt cgctcatgga acatctttca
    17221 acaaaactcc tcaactcaag aatgcagaga gatttgaccg attcaaccgt tctcagaaac
    17281 atcggaactg ttttcggaca tattctcata agcatcaaaa actgtctgaa gggtctttca
    17341 aaaattgaaa taaatgaaaa aaagataatt gaagaccttg atgacaacct tgaactcctt
    17401 gcagaaccag tgcaaaccgt catgcgcgtt tacgaggagg aaaaccctta tgaaaaactt
    17461 aaaatgctta ccagaggcag aaaaattgat gttgaatcgt tgcataagtt catagattca
    17521 cttgaaaaag tcccttcaaa cattaaaaaa gaaatgaaag atctgaaacc ttcggactat
    17581 accggaattg ccgaaaaact agttgatctt tattttaaaa aacgggaatc tctgtaaatt
    17641 atttgacagt cagttgaatt ctttaatact ttttgataga ctatggcact aaacattccg
    17701 gcagaatgtt ctgttttttt ggaggattga aatgaaaggt tatatttttt ttcccgtttt
    17761 gttttttctg ctgataaaca tatcctgtac ttctgatggg ctgaaggaag atttttccga
    17821 catacccgac tgggattatt caaatgttgt taccgatgaa ccttcgggaa atcaggacga
    17881 caacgactca gaagaaacac ctgataactc aaactcaact gatcctgctg atgaaaacat
    17941 aagcacggga gatcaggagg aaacaactga tgacggcggc actattcagg gatgcggcaa
    18001 tgaaaaggtc gaatacgaag aaaggtgcga cagcactgtt cccctcacct gctcaagttt
    18061 aaatcccaat atgagcggcg tgacctactg tatgaacaac tgtcagggat acgacatgca
    18121 aagctgcggc agatcagata aatcatgggg tttgctcaat ttgagatttc tttcaaactt
    18181 catccttgac gaagcaaaga tcggagatta ttcatatttc agcaaagggg ctcttcccta
    18241 tgcggcattc aatggtgttt acggagacac agtaacaata ttcccgacca tcggagaaaa
    18301 tgtatctttt gcggtgacca acaactataa agggacgctg ggcggcaata aaagacagct
    18361 gacgattaaa cagaacgctg ttgcgggcgg catcgggtat cccagacatg aactggaatt
    18421 ctctccaggc actatttcaa agggttccga atacagaata aacgccgtta ctatctttga
    18481 tcttgcggac aaccttttga aacttgtgag ataccgtttg atagaaaaaa aggacggagt
    18541 tgaatgcatt atgggaatag gttattccgg aaccgtttac ataaataaca tcgttcccga
    18601 caacgccgat cttttcgacg gcgggatcat cgaagttgtt gcgaataatg tcgatttttt
    18661 ccatccttca gaattcccgg ggcttgatga agaaaacccg gaagttccgg cagagatact
    18721 taaatatcct gtgtgcgaca aataatatta tatgtatttt gaccgtatta atgaaaccat
    18781 ctgcgccctt gcaacatctt caggaagttc aataggaata atccgtatca gcggaaaaaa
    18841 ctcttttaaa atagcatcat ccctttgcgg gataaagtct tttgaacata tgcgggccaa
    18901 gatagtaaag ataatccttc agggaaaaac tcttgaaaaa tgtcttctgc tcccttttaa
    18961 agcaccgaaa agctttaccg gcgaagatgt cgttgagttt cacattcacg gttcaaacaa
    19021 aaacggagag gagataatgt ctcttctgct ggaaaaagga gctgttccgg cattaagggg
    19081 tgaattttct ttcagggcag tcatgaactc aaaaatggag ctgtctcagg cgtttgcgct
    19141 taacaccctt ataacttcat caaaccctgt ttctgttgag ctttcaagaa aggccgcctt
    19201 cgaagacaga gccctgaaaa aactttttct tctcttaaat gaatgggaac atttttttgt
    19261 acttaccaca gcgatcattg attttcccga tcaggttgaa aacgaacttc ccgcacataa
    19321 actttcagaa cttgctgaaa aaacggaaaa atatctttct gaaacacttg acaactccgc
    19381 tcttcttgaa aagaccgaaa ctttttctgt cttaattgcg ggcaaaccca atgtgggcaa
    19441 gtcctccctt ttcaacatcc tgataaacaa ggatcgcgca atcgtttctc atgatgcagg
    19501 aaccaccagg gattatatta cagattcttt aaatataaac gggatcggcg tacaggtgat
    19561 cgactctgcc ggattaaggg aaggtttgtc tgaaaccgaa cagcagggga tcgacaaaag
    19621 caggcggctt ataaaacatg cagatcttct tcttcttatg ttcgacggct cccttcctat
    19681 cgaagaaaaa gacttggaac ttttaactga aacctcgata tatccaagga ttctgatcat
    19741 taacaaaatt gacggacacc aaccggaagt gttcccggaa gaatttaaaa acgctctgaa
    19801 aataagctgt cttgaggaaa aagggatcaa cgcggtcatt gaagcaattc aagaacaaat
    19861 aaaaaagacc atgcctgaca aaaataaaga cctgtttttt tcaagctggc agaaagatct
    19921 gacggttgac acacttgaac agatctcaga actagaaaca tatattataa gtggcgaaat
    19981 agaaattatt aatgttctca taaaaaaaat ctttgagaac ttaaaagacc tcaccggaac
    20041 gatctcgtcc cactcagttt acgacaggat cttttcagga ttctgtcttg gcaagtagct
    20101 tttccattca tataaaccgg actcgtttaa acgaaagcat tttactttta acgcaaaatg
    20161 tggtatcagc catacctgaa ccccggcttt gctctgtttt tttgttgtgg gtgattttag
    20221 aaactccctt ttcagggtgg cggaatggat tattttgatg tggcagtcat aggcggcgga
    20281 catgccggaa tcgaggcatc gtatgcatct tcaaaaaccg gtgcatcaac tcttctaatt
    20341 acaacttcaa ttgaaaagat cggtgagatg tcctgcaatc cttcaatcgg cggacaggca
    20401 aaaggccaga tagtacgcga aattgacatt ttcggcggct taatgggtcg tgcggccgat
    20461 tattccgcga tacaatacag aattctaaac cgcagaaaag gaaccgcggt acaggcatta
    20521 agggcccagt gtgataaaaa tctgtataag gaatttgtca ggaattttct tttccaaaca
    20581 ccgaacctta aaatttttca aggagaggct ttttctcttg agcatggaga aaattcactt
    20641 tttaaaatca caacttcagg cggcagtttt tttcaatcaa aagccgtaat tgtcaccgcg
    20701 ggaacatttt taaacggaac tgttcacatc gggcttaaca atttttcaag cggccggctc
    20761 ggtgaacccg catcgtcagg tctttcggaa tatatatccg gattcgggca caaaaaggtc
    20821 cggctgaaaa ccggaactcc tgtcagaatt cttggaaatt cgattgattt ttcaaaaatg
    20881 gagacacagg aaggagagac agacataatt cctttttcga tattttccgg ttctgtttcc
    20941 aaaagacaaa taccctgcta tttaacaaga acgactgatc tcacaaaaaa gatcatactt
    21001 gaaaatcttc atttttcccc aatgtacgga acttcaaagt ctattgaagg aataggtccc
    21061 agatactgcc cttcgatcga agataagttt gttaaattcc ctttgaagga aagtcatcag
    21121 atatttgtcg agccggaagg ttgggactgt tttgaatatt accccaacgg gataagcact
    21181 tctctccctt tcaatgtcca gaaagaattt ttaaagacca tccccggact tgaaaatgct
    21241 gtcataacac gacccgcata tgcgatagaa tacgattctt ttgaccccac cgatattgat
    21301 cttacgctta tgagccgttt tgttcctgga ctttttcttg ccggacaggt gaacgggacc
    21361 agtggctatg aagaagctgc cggacaggga cttgttgcag gagtaaacgc tgcatatttt
    21421 tctaaaaaga acggaaagat ctttgtcccg gggagatcaa aatcctatat tggtgtgatg
    21481 ataagtgaca taacatccaa gggggtagac gagccctaca gaatgtttac atcaagagct
    21541 gaatacagat tgtcaatacg cgacaataat gttgcggaaa gacttcttga actttctttt
    21601 tcaaatggac tcatttcgga aaaagtcttt cttgaagaaa agaggaagtg ctcccttgta
    21661 tcagaacttg taaccatgct taatgagaaa atcatatatc ctgacaaaga aaccaacgcc
    21721 cggatggaaa aactttcgga aacacccctg aacaagccgg tatccctttc aactgttttg
    21781 aaaagaccgt ctgtgggcag aaaagagctt tttcttatat cgggatatgt ttccgatgcc
    21841 ggaaatgaaa tatggtccag agcggaaaca gaaataaaat acagctcctt tgttgaaagg
    21901 gaaaaggccg agatccaaaa gtttgaagag ctctcctcca tatccattcc cgacggattt
    21961 gattttaatt ccataagcgg ctttacaaac gaaataaaac aaaaactttc cgccagaaga
    22021 cccaaagatc ttgcacaggc atcaatgatc cccggaataa ctccggcagc tatttcaatt
    22081 ttaataatgc atttaaaaag agacaaagca tgacagaaaa atttccggaa agctgtgttt
    22141 catgtgaaac atttcacaga aagatcaacc tgctgcttaa tccttacgat gtttccattg
    22201 atatcgaatc tgcagaaaaa ctcgtttctt tctggaattt tttctcccac tggaacaagg
    22261 cacacaacct ttcatccgta acagatctcg aggaggcgac tttcatccat tttgcagact
    22321 cactctttcc tgcctcggaa aaagaaattt ttttaaaaga aaagcgggtt cttgatttcg
    22381 gcacaggtgg cgggtttccg ggaattccgc tttcattgta ttttaaggat tgtcagtttt
    22441 ttcttcttga taaatgcagg aagaaaacat ctttcatttc ttttgctgcc gccagtctcg
    22501 gcattaaaaa tgtttttccc gtcaatgctc ttcttgaaaa acacaaaggc acaaaatacg
    22561 atataatcac ttcccgcgct gtaaagatcg acaaaaaaat gtttgatcta tgccgcagca
    22621 ttttaaatga agacggctgg ctaattattt attattcgct cagtcagaaa ccggttgaaa
    22681 gcgagttttt aagccatgtc
//
LOCUS       DAOOYB010000009        22427 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_2145_length_22427_cov_9.158859, whole genome shotgun sequence.
ACCESSION   DAOOYB010000009 DAOOYB010000000
VERSION     DAOOYB010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 22427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22427
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2145_length_22427_cov_9.158859"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="PL056_01165"
     CDS             <1..301
                     /locus_tag="PL056_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45654.1"
                     /translation="GCSAEVKILGEYVAVKNDPELFDLLKKISLEKGIRCIEKKGELV
                     GEDFGYFTQKWGGLLFWLGSKESGTDPNPLHSEKFFPSFKAIDAGLEIMREILLY"
     gene            382..738
                     /locus_tag="PL056_01170"
     CDS             382..738
                     /locus_tag="PL056_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45655.1"
                     /translation="MSTGKIIQIVIALLIIYVGWFVAKPYVDKYRLEKVVENIAQYCT
                     INTVEKTEQEFQNRIVDLGRKDIIPGMLHLEKDEETQRVRAVLKYKDEIKVFGHVFKP
                     LEFTIIKEAAKVDKII"
     gene            740..2017
                     /locus_tag="PL056_01175"
     CDS             740..2017
                     /locus_tag="PL056_01175"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HPM45656.1"
                     /translation="MKLVIKNGIIVNPAGKINGKTALVIEDGMIAEITENPDMSGAEV
                     IDAQGLIVAPGFIDIHVHFREPGYEHKEDIMTGLNAAAAGGFTSVCTMPNTNPVPDNA
                     ETVKLMIEKAAKGNGVRLYPVASATKGIKGVEISDADELKKAGAVALSDDGRPVQNTA
                     LLKKIMEKGRSCGMKYVSHSEDLYLAEGGQMNEGHFSAVYGMKGISVVGEASQIARDC
                     MLAEYLDVPLHISHVSAKMSVDMIRYFKSRGVKVTCETAPHYITLTDEAVRKFGTNAK
                     INPPLRTENDRLAMIEALKEGVIDAIATDHAPHHQKEKEVEFSSAPCGIVGLETALPL
                     VLMLYHEGILTIERVVELFTAGYRIMGVPGGIIEKGAIADITIFDPDRQWTIDKDRFM
                     SKSRNTPFHGFNVQGVVFATIADGKIIYKRDFR"
     gene            2014..2631
                     /locus_tag="PL056_01180"
     CDS             2014..2631
                     /locus_tag="PL056_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45657.1"
                     /translation="MSAVKTRIYSSFDEAEAALLHEFKKEAFYFTGEDLKNFNFWQQY
                     FNLFFIYHYDIGEGKTLAEKRELEKASVMLFSPSLFSRLKGEDLFTGEKLVLSEDEEN
                     SLTWYKEKEFILSFFHQREDGIHFVESRSALLMHVKAAGFFKKLKKALDEGSYFPSAN
                     YILFLLSILMMKKERFASSKLYDPYQFEEIEKLHLYYEKFAKLLI"
     gene            2603..3502
                     /locus_tag="PL056_01185"
     CDS             2603..3502
                     /locus_tag="PL056_01185"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPM45658.1"
                     /translation="MKNSLSSLSETRLNELVQKAATLFERGECCPVNCGGGDTRPCGA
                     VKEGIKVASFAVHEGEEPPVSGWSGAGNIFFSGCSAKCVFCQNWPISHENNGKIYSIE
                     EFGQNIIKLIRKNVHNINLVTSDHYLGRVLESFKNIRNELNVPVVYNCNGAHTEELFK
                     IILEVSDIFLFDVKYATDEPSAELSCIKDYVKYNSRCLEALLEKKIPWVENDLGILCK
                     GLIIRHLVLPGYIDNSLQVVELLNDYRKRGLDFKLSLMSQYFPAHLAHGIEKINRRLT
                     PEEYSKVTDLVEKYEIDGWIQEF"
     gene            3562..4173
                     /locus_tag="PL056_01190"
     CDS             3562..4173
                     /locus_tag="PL056_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45659.1"
                     /translation="MEKKYFVIIAFAAFFYSCSEKKIGDGYGLCKEGETRCTGNKLEV
                     CSIFKEWSLKSECDFYGGECVTDGKNSYCTESGNDKELSDGYFDWGEEEDDEYIEEDD
                     DNIIYDYDGYDEDTVDSEETDEDIDEDIDDAILDEDVQDNDIIDEDVVEPVCGNGELE
                     TGEVCEKGDLTDCTEIDPEKYSGGKAFCLDDCSGYNTITCSEK"
     gene            4173..6368
                     /locus_tag="PL056_01195"
     CDS             4173..6368
                     /locus_tag="PL056_01195"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPM45660.1"
                     /translation="MKGFIFLFLLSVLFSCEKTDDTFVSCGGTVISVSGRAEDDLYVK
                     SSFNGWSLSTPMNFKDGRWSVELFLNPGDYPYSIYSAKSKKSFPDSENPLTMFDRNLR
                     YSRLVVSDCRYPKIELVKRPVINGTDISFEIKYNPGISGGYPDLKKSVIKLNNEVQSY
                     QFDKKSGTFSINFTAPEKGKYTWYFKIYDTDGFASEVLTVPVWIEEKEFSWEDAFIYQ
                     IMTDRFHNGDKTNDSALKDIDEKANWQGGDYAGIIKKIDENYFSDMGVNAIWISSPVK
                     NTDNPGKGMGGDTRYYSAYHSYWPVATGWSDTVQIPGLDSPLEKRFGTAEELKELIQK
                     AHKQGIRVMLDFVPNHVHTDSYLWETYKNLGWFHMASYGQPANANGGYTCGWEHPVEC
                     WFTDYLADIDYRNIDAMNSVIDHLIWLITEFDIDGLRLDAIRLMVIDFTTTLKTAIQR
                     KVTTTGIPFYMIGETFTSDTGWDEIGFYIGERKLDGQFDFPLYHHIVRTFLLQSEMIP
                     ELVEFLEENDNRYKNDFYELAVMGNFIGNHDVARALSIANNDFDGVSSSGGDEAHEKV
                     WENSPVSPETEEPFKKMRNALTFLFATTGIPVIYQGDEYGMPGANDPDNRRMTLFGDD
                     LSDFQKKNLEHTQILGKFRAEKRSLKHGVRKNLIIADNFYCFAMVKDGETVIAAFNNS
                     DLQVVEKIDVSNLSGVEGDMTGLFGGNTFKISEGHIEIELEPFSSELIF"
     gene            6379..7338
                     /locus_tag="PL056_01200"
     CDS             6379..7338
                     /locus_tag="PL056_01200"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HPM45661.1"
                     /translation="MRIDEFIKNRSNFFFSLEVTPPDRGRSVTEIFETIDILMPFDPK
                     FINVTYHQPHISYKTENGIKSRFVYRKKPGTVGICSAIQSRYGIDAVPHLLCGGVDKW
                     EIEDTIVDLNYLGFDNIFALRGDPAFGMTEFVPKEDGYRYASELVAHISRMNSGCYLD
                     DELKDGIKTNFCVGVAAYPEKHIEAESIENDIGNLKKKVEEGANFIVTQMCFDAEVYV
                     RFVNKLRNIGVTVPVIPGVRPLTSSAQLEIIPSTFGVSVPQKLKETMSNAKTKSEEFE
                     AGTSFVADLCRELIEKGAPGIHLFTMGKGKSAKALFERIKAGV"
     gene            7340..8275
                     /locus_tag="PL056_01205"
     CDS             7340..8275
                     /locus_tag="PL056_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008619019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPM45662.1"
                     /translation="MKRVIVTGGAGLIGSHLCRRLLKEGYDVVCVDNFYTGSKHNIAD
                     LLKNPYFELITHDVEESLMINASMIYNLACPASPVHYQKDPVKTVRTSVFGAINMLEL
                     AKKSNAKILQASTSEVYGDPTVHPQNEEYWGNVNPVGLRSCYDEGKRCAETLFFDYWR
                     QFGVKIKVARIFNTYGPAMSVDDGRVISNFILQALRNEDITIYGDGTHTRCFCYVADM
                     VDALVNFMNLDDEVTGPVNIGHTEEISMKQLAEIIIEMSGSKSSVKYFPLPENDPQRR
                     KPDTSLIEKLINWKPVTPLEEGLNATIKYFKELQS"
     gene            8272..8994
                     /locus_tag="PL056_01210"
     CDS             8272..8994
                     /locus_tag="PL056_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014489243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPM45663.1"
                     /translation="MKLSVLIPVYNEENTVENLIKKVAQIKGQGNIKDLEIIAVNDCS
                     KDNTIEILRNIEKSGLIKLVHHELNMGKGAAIRTAIAHATGDFTIFQDADLEYDPNDI
                     VKMAGVVEEKGLDILYGSRFLAKKESPLGKFHYFVNAGLTTLSNFFTGLKLTDMETCY
                     KMFKAEVIKKIEIEENRFGLEPEITAKAAALVASENLKMEEIPISYIPRKHNEGKKIG
                     FRDGIRAVYIIFKYGFKTENML"
     gene            8991..10061
                     /locus_tag="PL056_01215"
     CDS             8991..10061
                     /locus_tag="PL056_01215"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HPM45664.1"
                     /translation="MKIFFIFILCTLFLSCSTAKKNENVDDSSDSELSDVDTVIGEEE
                     YTDEDDESGIASGTVCTGQAKCYDDLLEITCPSSGNDFYGQDAQYRDLCSSAGFVVTS
                     SEKGVADSFTGLFWQRGLPDIYEGCTGGEPAGSLCLWQEAIDYCKNLEEDGFSDWRLP
                     AIVEISTLPDYGKSIPAIDQLYFPETIPDYFWSSTVHKGFGDYSWVAYFNDGNADRFL
                     QSKAHNVRCVRGRTFKNNTKFIRETFNGEYVLVNERTNLMWADTCSNSQTWKDALKYC
                     QDLVSAGFSDWRLPNVNELKTLLDYSLEDPATTFPSMPSQPFWSSTTYSGYSEYGWYV
                     SFMYGEVYYHTKSNFYHAKCVR"
     gene            10134..10763
                     /locus_tag="PL056_01220"
     CDS             10134..10763
                     /locus_tag="PL056_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45665.1"
                     /translation="MKVVFVIFTFILSFSGCSDFLDSSAGKLGEKCFGNGTCRSDLIC
                     VDNVCVESIVGENDEDAEKYDDQSEQDSETVDEKNDETDEEDEDEAEFDHDAVDDDDL
                     SDEVLTESDEIPDEGDDTVPDEDADEVPDDAFDCSDAYFGGGTGTFSDPYIVSTPFHL
                     NSVRCFLIGHYFRQTSDINLSGYTTGEGWDFSVTWESDGNTNEGYMYLK"
     gene            complement(10772..12484)
                     /locus_tag="PL056_01225"
     CDS             complement(10772..12484)
                     /locus_tag="PL056_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45666.1"
                     /translation="MKKILKVSFIIIAFFSFLSAQDAEMTDAVQIPDTEEEDDTMTDS
                     EQPDDETADAESKTDPQTAAQTEPEAQPEPEPEPEKVVEQFIPVWLSEEHGLIDLSRE
                     WNSKLKIFPEMPVFLTARFYKGNVENYKIIILFVHSDGHLEETELVVTEPVITGIRDK
                     VAAYTMEEKKVETAQKDMKEIVKSTDRTLLKNDMGVMAELLAASIMYSGGWGASLPWI
                     FGGEKATAKAYLSSSLLGAGIGFFAPFLALRKSDLSTGGAIGAELGGLRGALDGFLLF
                     SLLAGFDDHDTTKVRGMTGLITSISITEYITGMYIADRMKISEERMRAVTLYSFTGYL
                     AGLELFLLAAGDGGDENLSSISRKDGIRILSGAMLAGGVAGMTAGYYLSGKAHYTNGD
                     TVIAGTTIPVVAGLPLALASLGDDVDARIYAGVSLAGTIAGAVTGHYFAKTMDFKSWQ
                     AVMVDLGTIGGALIGFGLAYLIGDDEIFRNTGKYTVPIAVGGAVGFAALMAVFAKDAK
                     KQKIEHESSILSGFKINLNPYAFTALDRNHRVNEKTLQDPQLIENYISEYSSNTMLNL
                     SYEF"
     gene            complement(12493..13176)
                     /locus_tag="PL056_01230"
     CDS             complement(12493..13176)
                     /locus_tag="PL056_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45667.1"
                     /translation="MKTKFLISLIILITFIPLWAEVEEVKEKKPDKIEIALNGGLSMK
                     FSALGNNGGFLSSDTEFAYLVGFKAAVLFNKSWFVGGAGYFLGNDIGWRCEELSTSYN
                     YDNYESPCYKSQYRDTYNKGSLSLGYGGLTAGYTFNIKSFFRVETGFLIGGGRFSSMN
                     ENGDYYFSQSFFALEPQMDLLFVITRFLALSVNVSYRLISGMGQSSVYSTADMSGPTV
                     GLDIRLGSF"
     gene            complement(13188..13874)
                     /locus_tag="PL056_01235"
     CDS             complement(13188..13874)
                     /locus_tag="PL056_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45668.1"
                     /translation="MRRSFLFVLTFFIFFNFILSGEEKSEKPSTIADALKAGNIDVGG
                     YGALSMKFTGIDQNDGFLSDESFFAYMIGARGGILLDHKWTIGAGFYVLTNNFYYRCP
                     KEDRSDFCTDDPYRNKMILGYGGLYVSYLAEIKKFMGIDLGFLAGGGSMKGKKSEDDD
                     HWYNDEYGAEYSFFIFEPEIQLVAKFTGYFGMAIGLGYRLTVPDSKSAYSITDMSGPF
                     ISMEARLGVF"
     gene            14052..15335
                     /gene="eno"
                     /locus_tag="PL056_01240"
     CDS             14052..15335
                     /gene="eno"
                     /locus_tag="PL056_01240"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HPM45669.1"
                     /translation="MLEIIRIKGREILDSRGNPTIEVEVELANGVMGSAIVPSGASTG
                     VHEALELRDGDKKRYSGKGVMKAVENVERIAAEVVGMSALDQRLLDETMIELDGTDNK
                     SELGANSMLGVSMAVARAAAASLGIPLFKYIGGLRSDTLPVPMSNIINGGSHADNNVD
                     LQEFMVMPVGAESFREGIRMNAEIFHTLKKILKDKGLATGVGDEGGFAPNLKSNEEAL
                     DVIVEAIKKAGFVPGKDIVIALDPAASEFFEDGKYVFKKSSGKSMTSAEMVDYWESLV
                     NKYPIYSIEDGMAEDDWDGWALMTERLGSRIQIVGDDLFVTNVERLGEGIERGVANSI
                     LIKLNQIGTLTETLDTIEVAHRAGYTTVISHRSGESEDTFIADLAVAVNAGQIKTGSL
                     CRSERIAKYNQLIRIEELLDEIAYYPGTDILFSEE"
     gene            15440..16030
                     /locus_tag="PL056_01245"
     CDS             15440..16030
                     /locus_tag="PL056_01245"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPM45670.1"
                     /translation="MNEESAVKKNILDAARKVFSQYGYKKTSMSDIAKESGKAKSSLY
                     HYFESKEAIFVTVIKEKIDLLFNSSVEEASKAADPVGRIHAFIFSGKENINKICKEFG
                     DILLTELFDFLPLIKETILEYVNQRLQFMKLIIDDGVGKGIFKTDNSFELAFIIEFSF
                     WGFSENHIMGNFFGNSKINPDVTEKLFDTLISGISV"
     gene            16357..17559
                     /locus_tag="PL056_01250"
     CDS             16357..17559
                     /locus_tag="PL056_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPM45671.1"
                     /translation="MRNLKIFRKIDDYDRVSLARKNGIYPYFREIMSEQDCEVMLRES
                     GRVIMLGSNSYLGLQSHPEVKEAAIKAIEKYGTGCAGSPFLNGTLDIHKELEHELADF
                     VGKEDAVLFATGFMANLGTISTIVSRHDCILMDKYDHASLIDAAELSYGKIVKFKHND
                     CRDLEAKLSYVDGKWGKLIAVEGVFSMDGDIANLAEITEIAEKYECAVMVDDAHGIGV
                     MGKSGRGTADYFGLTDKVDIIMGTFSKSLASIGGFVASDAQTIDFLRHHSRSVIFSAS
                     ATPASTASVLASLKIIRREPERIEKLWENTRYMKNKLKEAGFKTGKSETPIIPILVGE
                     DETTFVLCKNLEKDGVFVNPVISPAVAKGSALLRVSLMATHTFDQIDFSIDMMKKNFN
                     KMGLLHDN"
     gene            17549..18670
                     /locus_tag="PL056_01255"
     CDS             17549..18670
                     /locus_tag="PL056_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45672.1"
                     /translation="MIIESVTSSRRGVDDFIRLPFTLYNNEKNWVPPIKFVMKEKFNK
                     NKGAFAANNKNELFVAYRDGRPVGRVSAQIDKVYNEFYSSKTGFFGFYESENDPEILK
                     SLMEHAEKWLKEEGCDECTGPFNPNINDECGFQIEGFDRRPTIMMPYTKPYYPDLFEG
                     YGYSVAQVLHSFTIDNIEKTPEIVSRISAQIRKKHSDIEIRKINMQNLESEMKMILSI
                     YNDAWHDNWGFTPMTKPEIEELVATLKLFADPRVIYLLFKGGEPAACLVAIPDLNELV
                     IKNRNGTLNPSFLFDCLFHKRSLTSLRVIIMGVLRKFHGLGLDILLYNEIFNDGLKTE
                     NYRNVEMGWVLECNMKMNSTLRKIGAQCPNKYVIMRKSL"
     gene            18673..19653
                     /locus_tag="PL056_01260"
     CDS             18673..19653
                     /locus_tag="PL056_01260"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPM45673.1"
                     /translation="MKKVIVTGATGFIGSHLVSKLVKMQGVSLTVLTRRMTKDLATLE
                     KSGVEVVLCELSDVHLMKNRFRGVDCLIHLAGATKTLTEKEMFEVNAGYTENLLKSCE
                     LGTHFVLVSSQAVAGPAKSKDKPVTESDLPNPLTWYGKSKLEAENEVKEWGKSSIFTI
                     VRPCSVYGPGERDIFSYFKLVKSGLSLTVGFKEKRLSIIYVDDLVNAILHLAFNVKPS
                     GTTYFASGDEDCSWKEFSATVSEAMGKKGVIPVKLPEFASLPVALVSEFIAKMTEKAA
                     LLSFQKIIELRQDYWLCSNALLKSTGWKPEYTLQKGVDESVRWYRLNGWL"
     gene            19742..21991
                     /locus_tag="PL056_01265"
     CDS             19742..21991
                     /locus_tag="PL056_01265"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HPM45674.1"
                     /translation="MGVKFGTFKGVFVPSTEAILGTVLFLLMPVLIADVGLWAMLGVI
                     ILAHTVTFATSFSIADCATNLNNIGPGGMYALSRRSLGKAFGGSIGFQLYLAQAASIG
                     FYSIGFAEPLQSIIAPYLDFIPIFTLTDPASILIQKQILASVFFFLFFIVVMIGADFT
                     LKIQTFILFALGAGILSVMISPFFGITFNNLPVFSGSFNFSGAREITLGLFFMTFAQF
                     FPAVTGIDAGIGMSGDLENPRRSLVKGTFYAIGITFVIYLIIAVVYAMLDKNIMIQSY
                     MNGAPVPVLLTEIMSYSSAFPVNILGMFLVTGILFATGSSALSVFMTAPRTLQSLTIN
                     GVLPAKLSFLSGDFKKGGNEPRFAVIVTSFIGLFVIWAGNINTASMIVGILFLVVYGW
                     INGSAFLERASGNPGFRPTSRNHWSVSLYGFLISVVSIMLFDPMIGILIIISQYFMFR
                     AILRYKAGNRIEGVWWGIIFSLATRWLKMLGNVVQGSKNWRPVMTALVISEKGSSPER
                     TAYLGSFLAGHKGMVNLNIIGNEAEFNSYGIPYSFIPSDDINETAMALLMTSHPTGVE
                     INTVLIEYVKKLHTAKVITEMISKKRNILLFQNSSKLTKIENIDIWWRGEMNGNLMVL
                     LAYIITLDRREKCCNPKIRIIRMAQSNESVDTDRIEMETLLENARLNGEVVILENENS
                     DFLDVLKKYSSSADLIMMGLPGNFAKESKMKIFNINEYFFDKQISKYDDLPPILFIKS
                     AQYIELTEQ"
     gene            22001..>22427
                     /locus_tag="PL056_01270"
     CDS             22001..>22427
                     /locus_tag="PL056_01270"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020772535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HPM45675.1"
                     /translation="MEVSKVKGLTDSERELFRYDLEIKKLGRQDFVSPLTQSKRIYST
                     QVIPENERIMAFIDKSVVDSIKEVNIDGPSFEKAGPREKLFFEPGETVSAVVTCGGIC
                     PGLNAVIRGIVVMNYYRYNNQRILGISNGYAGLVKEYGYE"
BASE COUNT         6835 a         4099 c         5062 g         6431 t
ORIGIN      
        1 tggatgcagt gctgaagtga agatacttgg agaatatgta gctgtaaaga acgaccctga
       61 gctttttgat ctgctgaaaa agatctcttt agaaaaaggg atcagatgta ttgagaaaaa
      121 aggtgaactt gtaggggaag atttcggata tttcactcag aaatggggcg gactcctctt
      181 ctggctcggt tcaaaagaat caggaacaga tcctaaccca ttgcattcag agaagttttt
      241 cccaagcttc aaagcaatag acgccggact ggagataatg agagaaatcc ttctttactg
      301 agctgttttt tttgacttaa tttaagtaat ctgatacttt tcgccggtta tcgttcaaaa
      361 ttttttcaag gagaaaaatt catgagtaca ggaaagatca ttcagattgt aattgcgttg
      421 cttattattt atgtcggatg gttcgtggca aaaccttatg ttgacaaata ccgtcttgag
      481 aaagttgtgg agaatatcgc tcagtactgc actatcaaca cagtcgagaa aactgagcag
      541 gaatttcaga acagaatcgt cgatctggga agaaaggata tcatccccgg catgctccat
      601 cttgaaaagg acgaagagac acagagagtc cgcgcagttt tgaagtacaa agatgagata
      661 aaagtgttcg gtcatgtttt caaaccgctt gaattcacaa taatcaaaga agctgccaaa
      721 gttgataaga taatataaaa tgaaacttgt cataaaaaac ggaataattg tcaaccctgc
      781 cggaaagatc aacggaaaaa ctgcactggt aattgaagac ggcatgatcg ctgaaataac
      841 tgaaaaccct gatatgtcag gggcggaagt catagatgcg caagggctga tcgttgctcc
      901 cggtttcatt gatatccatg tccatttcag agaacccgga tatgagcaca aggaagatat
      961 tatgacaggg ctcaatgcgg cagccgcagg cggttttaca tctgtgtgta caatgcccaa
     1021 cacaaatcct gttccggata atgctgaaac tgtaaaactg atgattgaaa aggccgcaaa
     1081 agggaatggc gtcagacttt atcctgttgc ctcggcgaca aaagggatca aaggggtaga
     1141 gatatctgat gcagatgaac ttaagaaagc gggagctgtt gcactcagtg atgacggaag
     1201 acctgtccag aatacagctc tccttaaaaa gatcatggaa aaggggagat cctgcgggat
     1261 gaaatatgtt tcccacagcg aagaccttta tcttgctgag ggcggacaga tgaatgaagg
     1321 tcacttctcg gctgtttacg gaatgaaggg aatttctgtt gtgggtgaag cttctcagat
     1381 cgcccgtgac tgcatgctgg ctgaatatct tgatgttcct cttcatattt cccatgtcag
     1441 tgccaagatg tctgtcgata tgatcagata tttcaaaagc cgaggcgtca aagtgacctg
     1501 cgaaaccgca cctcactaca taactttaac cgatgaagcg gtcaggaaat tcggtacaaa
     1561 tgccaagata aatcctcccc ttagaactga aaatgaccgg cttgcaatga ttgaagcgct
     1621 gaaagaaggg gtgatagatg caatcgcgac agaccatgct cctcatcacc agaaagaaaa
     1681 ggaagttgaa ttcagttcgg ctccatgcgg catagtcggt cttgaaacgg cccttcctct
     1741 tgttttgatg ctttatcacg aaggaattct tacaattgaa agggttgtcg agctttttac
     1801 tgcaggatac agaataatgg gtgttccggg cgggatcatt gaaaaaggcg ccatagccga
     1861 tattactata tttgaccctg accgccagtg gacgatagac aaggacaggt tcatgtccaa
     1921 aagcagaaat accccttttc acggttttaa tgtacaaggt gttgtttttg ctacgatcgc
     1981 agatggaaaa atcatttata aaagggattt tagatgagtg cagtgaaaac aaggatatat
     2041 tcctcttttg atgaggcgga agccgcgtta cttcatgaat tcaagaagga agcgttctac
     2101 tttacaggtg aagatctcaa aaatttcaat ttctggcagc agtatttcaa cctctttttc
     2161 atatatcact acgatatcgg tgaaggaaaa acccttgcag agaaaaggga acttgaaaaa
     2221 gcaagtgtga tgcttttttc tccgtcgctt ttctcaaggt tgaaagggga agatcttttc
     2281 acaggagaga agcttgttct ttcggaagat gaagagaatt cgttgacatg gtataaggaa
     2341 aaagagttca ttctctcatt ttttcatcaa agagaggacg ggatccattt tgttgaaagc
     2401 cgttctgcac ttctcatgca tgtgaaagcg gcaggttttt tcaaaaaact caagaaagct
     2461 cttgatgaag ggagttattt cccctcagcc aattatattc ttttcctgtt atcgatactc
     2521 atgatgaaaa aggaaaggtt tgccagttca aaactttatg atccatatca atttgaagaa
     2581 atagaaaagc ttcatctgta ctatgaaaaa ttcgctaagc tccttatctg aaacccgttt
     2641 aaatgaactt gttcaaaagg cggcgaccct ttttgagaga ggggaatgct gtcctgtaaa
     2701 ttgcggcggt ggagataccc gtccatgcgg ggccgtaaaa gaagggataa aggttgcaag
     2761 ttttgcagtt catgaaggtg aagaaccgcc ggtaagcggc tggagcggag caggcaacat
     2821 tttcttttcc ggctgcagtg caaaatgtgt tttctgtcag aactggccta taagtcatga
     2881 aaacaacgga aagatatatt caatagagga attcggtcag aacattataa aacttatcag
     2941 aaaaaatgtt cataatatta atcttgtgac aagtgaccac tatcttggaa gagttcttga
     3001 gtcatttaaa aatatcagaa acgaacttaa tgttcctgtg gtctacaact gcaacggagc
     3061 tcacactgaa gagcttttta aaattattct tgaggtgagc gacatctttc tttttgatgt
     3121 aaaatatgcg actgacgagc catctgctga actgagctgt ataaaagatt atgtgaaata
     3181 taacagcagg tgccttgaag cacttcttga aaaaaaaatt ccgtgggtcg agaacgatct
     3241 ggggatactt tgcaaagggc ttataataag acatctcgtg ctgccgggat atattgataa
     3301 ttcgcttcag gttgttgaac ttctcaatga ttatagaaaa agaggactcg attttaaact
     3361 gagtttgatg agtcagtatt ttcctgcaca tcttgcgcac ggcatcgaaa aaattaacag
     3421 acggcttaca cctgaagaat attccaaagt aactgatctt gtcgaaaaat acgaaatcga
     3481 cggctggata caggaatttt aagaaaattt gctctgaaaa cctgatgagg gtatattttg
     3541 caagatgatt tgaggtgaat tatggaaaag aaatattttg ttatcattgc ttttgcggct
     3601 tttttttatt cctgttcaga aaaaaagatc ggtgatggtt acggactttg caaagaagga
     3661 gagacccgct gtaccggcaa taagcttgag gtttgcagca tttttaaaga atggtcactt
     3721 aaatcagaat gcgattttta cggaggggag tgtgtaactg acggaaaaaa ttcatactgc
     3781 acggaatcgg gaaacgacaa ggagttgtct gacggctatt tcgactgggg cgaagaggaa
     3841 gatgatgaat acattgaaga agacgatgac aatataattt atgattacga tggctatgat
     3901 gaagataccg ttgacagcga agaaactgat gaagacattg atgaagatat cgatgacgca
     3961 attcttgacg aagatgtgca ggataacgat ataattgatg aagatgtcgt tgagccggtt
     4021 tgcggcaacg gtgaacttga aacaggtgaa gtctgcgaaa agggagattt gaccgactgc
     4081 acagaaatcg atcctgaaaa atattccggc ggaaaggcgt tctgccttga tgactgctcg
     4141 ggctataaca cgatcacatg cagtgagaag tgatgaaagg atttatattt ctattccttt
     4201 tatcagttct gttttcctgc gaaaaaaccg atgacacttt tgtttcatgc ggcggaacgg
     4261 tcatttctgt ttccggaagg gcggaagatg acctttatgt gaagtcctct tttaacggct
     4321 ggtcgctttc aactccgatg aatttcaagg acggtagatg gtcggttgag ctttttttaa
     4381 atccgggcga ttatccttat tcgatctatt ctgcgaaatc gaaaaaaagt tttcctgatt
     4441 ctgaaaatcc gctcacgatg tttgatagga atctccgcta cagtcgcctt gtcgtaagtg
     4501 actgtaggta tccaaaaata gaacttgtta aaagacctgt gataaatgga actgacattt
     4561 catttgagat taaatataat ccagggattt caggaggtta tcccgatctt aaaaaaagtg
     4621 tgattaagct taacaatgaa gttcaaagtt atcagtttga taaaaagagc ggtactttct
     4681 cgatcaattt tactgcgcct gaaaaaggaa agtacacatg gtattttaag atttatgata
     4741 ctgatggctt tgctagtgaa gttcttaccg ttcctgtctg gatcgaggaa aaagagttta
     4801 gctgggagga tgcgtttatt tatcagatca tgacagacag gtttcataac ggggataaaa
     4861 caaatgacag tgctttgaag gatattgatg aaaaagcgaa ctggcagggt ggtgattatg
     4921 ccgggatcat aaagaaaatt gatgaaaact atttttcaga tatgggtgtg aatgcaatct
     4981 ggataagttc tccggtaaaa aatactgaca atccggggaa agggatgggt ggcgacacaa
     5041 gatattacag cgcatatcac tcctattggc ctgttgcaac gggctggagt gatacagttc
     5101 agatccctgg acttgattct cctcttgaaa aaaggttcgg tacggctgaa gaattgaaag
     5161 agctcattca gaaagctcac aaacagggga taagggtcat gcttgatttt gttcccaacc
     5221 atgttcatac cgattcatat ctgtgggaaa cctacaaaaa tctcggttgg ttccacatgg
     5281 cttcctacgg tcagccggca aatgcaaacg gcggatatac ttgcggatgg gaacatcctg
     5341 ttgaatgctg gtttacagat tatcttgcag acattgacta tcgcaatatt gatgcaatga
     5401 acagtgtcat tgaccacctt atctggctta ttactgaatt tgacattgac ggattgcggc
     5461 ttgatgcgat aagactgatg gtgatcgatt ttacaacaac tttaaaaacc gcgattcaaa
     5521 gaaaagtgac aacaacagga ataccttttt atatgatcgg cgaaactttc acatctgaca
     5581 ccggctggga cgagatcggg ttttacatcg gagagagaaa acttgacggt cagttcgatt
     5641 ttccccttta tcaccatatt gtgagaacat ttctccttca aagtgaaatg attccagaac
     5701 ttgttgaatt tcttgaggaa aatgataata gatataaaaa tgatttctat gaacttgcag
     5761 ttatggggaa cttcatcggt aatcacgatg ttgcaagggc gttaagtatt gcaaacaatg
     5821 attttgacgg agtttcttct tccggcggtg atgaagctca tgaaaaagtg tgggaaaatt
     5881 caccggttag tcctgaaact gaagagccgt ttaaaaaaat gagaaacgca ttgacatttc
     5941 tttttgcaac aacaggaatt cctgtgatct atcagggtga tgaatatgga atgccgggag
     6001 ccaatgaccc ggacaacaga aggatgacgc tgttcggcga tgacttgagc gatttccaga
     6061 aaaaaaacct tgaacatact caaatactcg gtaaattcag ggctgaaaaa cggtcgttga
     6121 aacatggagt tagaaaaaat cttatcattg cagacaactt ttattgcttt gcaatggtta
     6181 aagacggtga aacggttatt gcggcattta acaacagtga cttgcaagtg gttgagaaaa
     6241 ttgatgtttc taatctgtca ggtgttgaag gtgatatgac agggttattt ggcggcaata
     6301 cttttaagat ttcagaaggc catattgaaa ttgaactgga accgttttct tcggaattga
     6361 tattttaagg agaggtgatt gaggatagat gaatttatta aaaacagatc aaatttcttt
     6421 ttttctcttg aggtaactcc gcccgacaga gggagaagcg ttaccgaaat atttgaaacc
     6481 attgatattc tgatgccgtt tgatccgaaa ttcatcaatg tgacatatca ccagccccat
     6541 atcagttaca aaacagagaa cggaataaaa agccgtttcg tttacaggaa gaaaccggga
     6601 actgtaggta tttgttcagc aattcagagc cgttacggaa ttgatgcagt gcctcatctc
     6661 ctttgcggag gagttgataa atgggaaata gaagatacga tcgttgacct taactatctc
     6721 ggttttgaca acattttcgc tcttcgcggt gatcctgctt tcggaatgac agagtttgtc
     6781 ccgaaagagg acggctacag atatgcgtct gaacttgttg cccatatttc aagaatgaat
     6841 tcgggctgtt atcttgatga tgaactcaaa gacggaatta agacaaattt ctgtgtcgga
     6901 gttgccgcat atccggaaaa acacattgaa gcagaatcta ttgaaaatga tatcggaaat
     6961 ttaaagaaaa aagttgaaga aggggcaaat ttcattgtta cccagatgtg ttttgatgcg
     7021 gaagtttatg tcagatttgt caataaatta aggaacatcg gtgtaacagt gccggtaatt
     7081 cccggtgtca ggcccctcac atcatcagct cagcttgaaa ttattccatc aactttcgga
     7141 gtttcagttc ctcaaaaatt gaaagaaacg atgtcaaatg caaagacaaa aagcgaagag
     7201 tttgaggctg gaacatcttt tgtcgcagat ttatgcaggg aacttattga aaaaggggct
     7261 ccggggatac atctttttac aatgggaaaa gggaaaagcg caaaagccct ttttgaaaga
     7321 ataaaagcgg gagtttaatt tgaaaagagt cattgtgaca ggcggagcgg ggcttatcgg
     7381 aagtcatctg tgcagacggc ttcttaaaga gggttatgat gtggtctgtg ttgacaattt
     7441 ttataccggc tccaagcata acattgcgga tctgttgaag aatccttatt ttgagcttat
     7501 aactcacgat gttgaagaat cgttgatgat aaacgcttca atgatatata accttgcatg
     7561 tccggcaagt ccggttcact atcagaaaga ccctgtgaaa acggtgagaa cgagtgtttt
     7621 cggagcgata aatatgcttg agcttgcaaa gaaaagcaat gcaaagattc ttcaggcaag
     7681 cactagtgaa gtttacggag atccgacagt tcatccgcag aatgaggagt actgggggaa
     7741 tgtcaatcct gtgggactta gaagctgtta cgatgaagga aagagatgtg ccgaaacttt
     7801 gttttttgat tactggcggc aatttggtgt caaaataaaa gtggcaagaa tattcaatac
     7861 ttatggcccg gctatgtcag ttgatgacgg tcgggtcatc agcaacttca ttcttcaggc
     7921 actcagaaat gaagacatca cgatatatgg tgacggaact cacaccagat gtttctgtta
     7981 tgtagctgac atggttgatg cacttgtcaa tttcatgaac cttgatgatg aagtgacagg
     8041 gcctgtaaat ataggtcaca cagaagagat atcaatgaaa cagctagcag agataattat
     8101 cgaaatgagc ggttcaaaga gttctgtgaa atattttcct ctccctgaaa atgatcctca
     8161 aagaagaaaa ccggatactt cactcattga aaaactgata aactggaaac ctgtgactcc
     8221 gcttgaagaa gggttgaacg caacaattaa atatttcaag gagttgcaaa gttgaagtta
     8281 tcagttctca tccctgttta taacgaagaa aatacagttg aaaacctgat aaaaaaagtt
     8341 gcgcaaataa aagggcaggg gaacattaaa gatcttgaaa taattgccgt caatgactgt
     8401 tcaaaggata acactattga aatattgaga aatattgaaa aatccggctt gataaaactt
     8461 gttcatcacg aattgaatat gggaaaaggg gccgcaataa gaactgcgat cgcccatgcg
     8521 acaggtgatt ttacaatatt tcaggatgcc gaccttgaat atgatcctaa tgatatcgtt
     8581 aaaatggcag gggttgttga agaaaaagga cttgatatcc tttacggaag ccgtttcctt
     8641 gcaaaaaagg aaagtcctct ggggaagttc cactattttg tgaacgcggg gcttacaaca
     8701 ttatccaatt ttttcacagg gctcaagctt accgacatgg aaacttgcta caaaatgttc
     8761 aaagcggaag tgatcaaaaa gatcgaaatt gaagagaaca gattcggact tgaacccgaa
     8821 attacagcca aagcggcagc acttgtcgca tcagaaaatc ttaaaatgga agaaattccg
     8881 ataagctata tccccagaaa acacaacgaa ggaaaaaaga tcggttttcg tgacggaatc
     8941 agagccgtct atataatttt caagtacggg ttcaaaacag agaacatgtt atgaagattt
     9001 tttttatttt tatattgtgc actctttttt tatcgtgttc aactgctaag aagaatgaaa
     9061 atgttgatga cagcagtgat tcagagttgt cagatgtgga taccgtcatt ggtgaagagg
     9121 aatataccga tgaagatgat gagtcaggta ttgcatccgg aaccgtctgc acggggcagg
     9181 ccaagtgcta tgatgatctt cttgagataa cctgtccgtc ttccggaaat gatttttacg
     9241 gtcaggatgc acaatacaga gatctttgca gcagtgcagg ttttgttgtt acatcgtcag
     9301 aaaaaggtgt ggccgacagc ttcacaggac ttttctggca aagaggcctt cctgatattt
     9361 atgaaggatg tacaggagga gaaccagccg gatctctctg tttgtggcag gaagcgattg
     9421 attattgtaa aaatcttgaa gaggatggtt tttctgactg gcgacttccg gcaatcgttg
     9481 agatttcgac ccttcctgac tatgggaaaa gtattcctgc gatcgaccag ctctattttc
     9541 ctgaaacaat tcctgattat ttctggagct caactgtaca taaagggttc ggtgattatt
     9601 catgggttgc gtatttcaat gacggtaatg ctgacaggtt tcttcaaagc aaggcacaca
     9661 atgtcagatg tgtgagagga agaacattta aaaacaatac gaagtttata agagagactt
     9721 ttaacggaga atatgtgctt gtaaatgaga gaacaaatct tatgtgggcg gatacctgtt
     9781 caaacagcca aacatggaaa gatgcactta aatactgtca ggaccttgtt tctgcagggt
     9841 tttctgactg gagacttcct aacgtgaacg aactcaaaac acttcttgac tattcgcttg
     9901 aagatccggc tacaactttt ccgtcaatgc cgagtcagcc cttctggtct tcaacaactt
     9961 attccggtta tagtgaatat ggctggtatg tcagctttat gtatggagaa gtctattatc
    10021 acacaaaatc aaatttctat catgcaaaat gcgtgagatg acactgtttt tttaattgaa
    10081 tcaatcccat tattaaatat aatatcccgt caattctttt ttaaagaggt accatgaagg
    10141 ttgtttttgt tatttttaca tttatcctgt ctttttcggg ttgttctgat tttctggata
    10201 gttctgcagg aaaactcggt gaaaagtgtt tcggtaacgg aacatgcaga agtgatctga
    10261 tctgtgttga taatgtctgt gttgaaagca ttgttggtga aaatgatgag gatgctgaaa
    10321 aatatgacga tcagtcagag caggacagcg aaacagttga tgaaaagaat gacgaaactg
    10381 atgaagaaga tgaggatgag gcggagtttg accacgatgc tgtagatgat gacgacttgt
    10441 ccgacgaagt tttaacggag tcggatgaaa tacctgatga aggggatgat acggttcctg
    10501 atgaagatgc cgatgaagtg cctgatgatg cttttgactg ttctgatgcc tatttcgggg
    10561 gagggacagg aacattttcc gatccataca ttgtttccac accgtttcat cttaattctg
    10621 tcagatgctt tctgataggg cactatttca gacagaccag cgacataaac ctttcaggat
    10681 acacaaccgg agaagggtgg gatttctctg tgacatggga aagtgacgga aataccaatg
    10741 aagggtatat gtatttgaaa tagactctat cttagaattc gtaacttaaa ttaagcattg
    10801 tgtttgaaga atattcagaa atatagttct cgataagttg cgggtcctgc aaagttttct
    10861 cattcacgcg gtgatttctg tcaagtgctg tgaaagcgta agggttcaga tttattttaa
    10921 aaccgctgag aattgagctt tcgtgctcga tcttctgttt tttagcatct tttgcaaaga
    10981 ccgccatcag agcggcaaat ccgactgctc cgcctactgc gatgggaact gtatattttc
    11041 ctgtatttct gaatatttca tcgtctccga taaggtacgc aagcccgaaa ccgatgagcg
    11101 ctccgccgat agttccaaga tcgaccatca ccgcctgcca tgatttgaaa tccatggttt
    11161 ttgcaaaata gtggcctgta acggcacctg cgatcgttcc tgccagagaa actcccgcat
    11221 atattctcgc atcgacatca tctccgagtg atgcaagagc aagcggcagt ccggcaacaa
    11281 cagggatagt tgttcctgcg ataacggtat caccattagt gtaatgagct tttccggaaa
    11341 gataatatcc tgccgtcatt cctgcaacac ctcctgcaag cattgctccg gaaaggatcc
    11401 tgatgccgtc ttttctgctg atactgctga gattttcatc tcctccgtct ccggcggcaa
    11461 gcaggaacag ttcaagtccg gcaagataac cggtaaagga atagagcgta acagctctca
    11521 ttctctcttc tgatattttc attctgtcgg caatgtacat tcctgttatg tattctgtaa
    11581 tgctgatcga agttataagt cctgtcattc cccgaacctt cgttgtatcg tgatcatcaa
    11641 agcccgcaag aagggagaaa aggagaaatc cgtcgagagc tccgcgaaga cctccgagct
    11701 cggcccctat tgctccgcct gtgctcagat cactttttct aagggcaagg aacggtgcga
    11761 aaaagccgat ccctgctccg agaagacttg aagaaagata tgccttggca gtggcttttt
    11821 cacctccgaa tatccacgga agtgacgctc cccagccacc tgaatacatt attgaagccg
    11881 caagaagttc cgccatgacc cccatgtcgt tctttaatag agttctgtcg gtcgatttaa
    11941 cgatttcttt catatctttc tgagcggttt caactttttt ctcttccatt gtgtatgccg
    12001 ctactttatc tcttattccc gtaataactg gctcagtaac gacaagttcc gtttcctcaa
    12061 gatgaccgtc actgtggaca aagaggatga ttattttgta gttctcgaca tttcctttgt
    12121 aaaaacgagc agttagaaaa acaggcattt cagggaatat tttgagcttt gaattccact
    12181 ccctgctgag atctatcaac ccgtgttctt cggaaagcca cacaggaata aactgctcaa
    12241 ccactttttc aggttctggt tcgggctcgg gttgagcttc tggctctgtc tgagctgctg
    12301 tctgaggatc tgttttgctc tctgcatcag cagtttcatc atccggctgt tcactgtctg
    12361 tcatagtgtc atcttcctct tcagtatccg gaatctgcac tgcatcggtc atttctgcat
    12421 cctgggcaga taaaaagctg aaaaacgcga taattatgaa ggaaacttta agaatttttt
    12481 tcatgttaca cctcaaaaac ttccaagccg tatatcaagt ccgactgttg gcccgctcat
    12541 atcagcagtg gaatagacac tgctctgtcc cattccgctg atcagacggt acgaaacatt
    12601 cacggaaagg gcaaggaacc gtgttatgac aaacagaaga tccatctgcg gttcaagggc
    12661 gaaaaagctc tgcgaaaaat agtaatcacc gttttcgttc atactgctga aacggccccc
    12721 gccgataaga aaacccgttt caactctgaa aaagcttttg atgttgaatg tatatcctgc
    12781 agtaagtcct ccgtaaccaa gagagagaga ccctttatta taagtgtcac ggtattggga
    12841 tttataacat ggagattcat aattatcata gttgtatgag gttgaaagct cttcacatct
    12901 ccatccgatg tcgttcccga gaaaatatcc agctccgccg acaaaccatg atttgttgaa
    12961 caaaacagcg gctttgaacc cgacgagata agcaaattcc gtatcagatg aaagaaatcc
    13021 gccgttattt ccaagagccg aaaatttcat cgaaagtccg ccgttaagag caatctcgat
    13081 tttgtcaggt tttttttcct ttacttcctc aacttcggcc cataaaggaa taaaagttat
    13141 cagaataatt aaagatatca gaaattttgt tttcaagtca acctccatta aaagacgccg
    13201 agccgtgctt ccattgaaat gaacggtccg ctcatgtccg tgatactgta tgcacttttg
    13261 ctgtcaggaa cggtgagacg gtatcccaac cctatcgcca tgccgaaata accggtaaac
    13321 tttgcaacaa gctgaatctc cggttcaaaa atgaaaaaac tgtactcagc cccgtattca
    13381 tcattatacc agtgatcgtc atcttccgat tttttccctt tcatggagcc gccacctgca
    13441 agaaaaccga ggtcaattcc cataaatttc tttatttcag caaggtatga aacatagagt
    13501 ccgccgtatc cgaggatcat tttattgcgg taaggatcat ctgtgcagaa atcggaccgg
    13561 tcttctttcg gacatctgta gtagaaatta ttggtgagaa cataaaaccc tgcacctatg
    13621 gtccatttgt ggtcaagaag tattccgccc ctcgctccga tcatatatgc aaagaaagat
    13681 tcatcagaaa ggaatccgtc attctgatca attcctgtga acttcattga aagtgctccg
    13741 tatccgccga catcgatatt gcctgctttc aatgcatctg caattgttga gggcttttca
    13801 cttttttcct ctcccgaaag gataaagttg aagaaaataa aaaaggtcag aacaaataaa
    13861 aacgatcttc tcattttaca ccccgttcaa actatttaag aatcgttgct ttccaaatct
    13921 tcaaaaatta aacagcaaca tcggataaaa atcaagttca aacttctttt aaacacaaat
    13981 aacattgaaa aaaattttaa aagtaatatc attctgcggt ttatttaaca attctttata
    14041 tggagagaac aatgcttgaa atcatcagaa tcaaagggcg tgagatcctt gactcgagag
    14101 gaaaccctac aattgaagtt gaagtcgaac ttgctaacgg tgtcatgggt tcagcgatag
    14161 ttccaagcgg agcaagcacc ggtgttcatg aagcgcttga gctcagagac ggcgacaaga
    14221 aaagatattc cggcaaaggt gtcatgaaag ctgttgagaa tgttgagcgg atcgcagccg
    14281 aagtagtggg catgagcgct cttgaccaga gacttcttga tgagacgatg atcgagcttg
    14341 acgggactga caataaatcg gaactcggtg caaattcgat gctcggagtt tcaatggctg
    14401 ttgcgagagc ggctgcagca tctctgggta ttccgctttt caaatatatc ggcggactca
    14461 ggtctgatac tcttcccgtg ccgatgagca atataatcaa cggcggatcg cacgctgaca
    14521 acaatgttga tcttcaggag ttcatggtaa tgccggtagg cgctgaaagt ttccgtgaag
    14581 gcataagaat gaacgccgag atattccaca cacttaaaaa gatccttaaa gacaaaggtc
    14641 ttgcaaccgg agtgggcgat gaaggcggat tcgcaccgaa ccttaaatca aacgaagagg
    14701 ctcttgatgt aatagtcgaa gcgatcaaaa aagcaggttt tgttcctgga aaggacattg
    14761 tcatcgcgct tgatccggcc gcaagcgaat tttttgagga cggcaagtat gttttcaaaa
    14821 aatcttcagg aaaatcaatg acaagcgctg aaatggttga ttattgggaa tctcttgtca
    14881 acaaatatcc gatatattcg atagaggacg gaatggctga agacgactgg gatggctggg
    14941 ctcttatgac tgaaagactt ggcagcagaa ttcagatagt aggtgacgat ctcttcgtta
    15001 caaatgtaga aagactcggt gaaggtatcg aaagaggagt tgcaaattca attctcataa
    15061 aactgaacca gattggaaca ctgacagaaa cgcttgatac gatcgaagtc gcacacagag
    15121 cgggctacac aacagttatt agtcaccgca gcggtgaaag cgaagataca tttatcgcag
    15181 acctcgcagt tgccgtcaat gccggtcaga taaagacagg cagcctctgc cgcagtgaaa
    15241 gaatcgcaaa gtacaatcag cttataagaa tcgaagaact tcttgacgag attgcatact
    15301 atcccggcac ggatatcctt tttagcgaag aataacctca tttttaattc ataaattcag
    15361 gatttattct aaaacttgac aaaacaattt tatttgctat tttcgaccgt aagaccgaaa
    15421 tggtcgaacg gagtggaata tgaatgaaga atcagccgta aaaaagaata ttcttgatgc
    15481 tgccaggaaa gtattttctc aatacggtta taagaaaaca tccatgagcg atatcgcgaa
    15541 agagtcagga aaagccaaga gttcactgta tcattatttt gaaagcaaag aggctatttt
    15601 tgttacagtt ataaaagaaa aaatagattt gttatttaat tcgtctgtgg aagaagcttc
    15661 taaagcggct gatcctgtcg gtagaattca tgccttcatt ttttccggca aggaaaatat
    15721 aaacaaaata tgtaaggaat ttggagatat tcttctaact gaactttttg attttcttcc
    15781 gttaataaag gaaaccattc ttgaatatgt caaccagcgt cttcaattta tgaaattaat
    15841 aatagatgat ggcgttggaa agggaatatt caaaactgat aattcttttg agttggcatt
    15901 tataatcgaa ttctcattct ggggtttttc agaaaaccat atcatgggta atttttttgg
    15961 taattccaaa ataaatcctg atgttacaga aaaattgttt gacaccttaa tatcaggaat
    16021 ttcagtataa gcaatgatat ttaattatag ttttttaatt aacagcttaa aaatttattc
    16081 ttttatcgac cgaaatgctg aaacggtcga ttcagttccg atttttagtg aggagaatgg
    16141 tgtataaagt ggttttttat tatgttgtta tttctttatt attttaaaga gttatgaatt
    16201 ttatgttttc gaattataaa ttttttgact ttttaagttt tttgttcaaa ttatctgtta
    16261 ctctgtattg cttgaaaagt gatgcaaaga aatgtcatgt agcggatagg tatagcagaa
    16321 aaagatttgc agaaatgata ttcactggga gggaatatga gaaatcttaa aatctttaga
    16381 aaaattgatg attacgacag agtttcactt gccagaaaga acggaattta tccctatttc
    16441 agagaaatta tgtcggaaca ggattgtgaa gttatgctgc gggaaagcgg cagggttatt
    16501 atgctcggat cgaacagcta tctcgggctt cagtctcatc ccgaggttaa agaagccgca
    16561 ataaaagcta tagagaaata tggtaccggg tgtgcaggat cgccttttct taacggaacg
    16621 cttgatattc acaaggaact tgagcatgaa cttgccgatt tcgtaggaaa agaagatgcc
    16681 gtgctttttg ccactggatt tatggcaaat ctcggaacta tttctactat tgtttcgagg
    16741 catgactgca tattaatgga caaatatgac catgcttcat tgattgatgc ggctgaactt
    16801 tcttatggaa aaattgttaa attcaagcat aatgactgcc gggatcttga agcaaagctt
    16861 tcatatgtag atggaaaatg gggaaaactg atagctgtcg agggagtttt cagcatggat
    16921 ggtgatatag ctaaccttgc ggagataact gaaattgctg aaaagtatga atgcgcagtc
    16981 atggttgatg atgctcatgg aataggcgtg atgggcaaat ccggaagagg tacagcggat
    17041 tatttcggtc ttacggataa agttgatata attatgggaa ctttcagtaa atcgcttgct
    17101 tcgataggtg gttttgtcgc gtctgatgct cagacgatag attttttgag acaccacagc
    17161 cgttctgtaa ttttcagtgc aagtgcaact cctgcctcaa ctgcttctgt gctggcttct
    17221 ttgaaaatta tcagacggga acctgaaagg atcgaaaaac tttgggaaaa tacccgttat
    17281 atgaaaaata aattgaaaga agccggtttt aaaaccggaa aatccgaaac tccgattatt
    17341 cctattctgg ttggcgaaga tgagacgaca tttgttttat gcaaaaatct cgaaaaagat
    17401 ggagtttttg tcaatcctgt gatttctccg gctgttgcaa agggaagcgc gcttttaaga
    17461 gtaagtctta tggctactca tactttcgat cagattgatt ttagtattga tatgatgaag
    17521 aaaaacttta ataagatggg gctattgcat gataattgaa agcgtaacct cttccagaag
    17581 aggcgtggac gattttatca ggctgccgtt tactctctat aacaatgaga agaattgggt
    17641 gccgcctata aagtttgtaa tgaaagaaaa atttaataaa aacaagggag cttttgctgc
    17701 aaataataaa aatgaacttt ttgtagctta tcgtgacggc agacctgtag gcagagtgtc
    17761 tgcacagatt gataaggtgt ataacgagtt ttactcatca aaaaccggtt ttttcggatt
    17821 ttatgaatct gaaaatgatc cggaaattct taaatcgctt atggaacatg ccgaaaagtg
    17881 gcttaaggaa gagggttgtg atgagtgtac cggtcctttc aatcccaata tcaacgatga
    17941 atgcggtttt cagattgagg ggtttgacag aagaccgaca atcatgatgc catatacaaa
    18001 accgtattat cccgacttgt ttgaaggata tggttattct gtagcgcagg ttcttcattc
    18061 tttcactata gataatattg aaaaaactcc tgaaatagtt tcaaggatat cagctcagat
    18121 aagaaaaaaa cattcagata ttgaaatccg taagatcaac atgcagaatc tcgaatctga
    18181 aatgaaaatg attttaagta tttacaatga cgcatggcat gacaactggg gttttacacc
    18241 aatgacgaaa cctgaaatag aggagcttgt tgcaacctta aagctgtttg ccgatccgag
    18301 agtgatttac ctgcttttta aaggtgggga accggcagca tgtcttgtag ccataccaga
    18361 tcttaatgag ctcgtgatta aaaacagaaa cggaactctt aatccgtctt ttttgtttga
    18421 ttgtcttttt cacaaaagat cgctgacatc cctccgtgtg attataatgg gtgttttaag
    18481 aaaatttcat ggtcttgggc ttgatattct tctttacaac gagatattca atgacggatt
    18541 gaaaactgaa aattacagaa atgttgagat gggatgggtg cttgaatgta atatgaagat
    18601 gaattccacg cttaggaaaa taggtgcaca gtgccctaat aagtatgtta ttatgcggaa
    18661 atcattataa aaatgaaaaa agttattgtt acaggtgcga cagggtttat cggttcccat
    18721 ttagtttcga aactggttaa aatgcaggga gtcagtttaa ccgtgctgac taggaggatg
    18781 acaaaagacc tcgcaacact ggaaaaatca ggtgtcgaag ttgttttatg cgaattgagc
    18841 gatgttcatt tgatgaaaaa tcgtttcaga ggtgtggatt gtctgattca tcttgccgga
    18901 gctactaaaa ctctgactga aaaggagatg tttgaggtta atgcaggcta tactgaaaac
    18961 ctgctcaaaa gttgtgaatt aggaacgcat tttgttcttg tgagttctca ggctgtggca
    19021 gggcctgcta aatcgaaaga taaaccagtc acagaaagcg atttgcctaa tcctctgact
    19081 tggtatggaa agagcaagct tgaggctgaa aatgaagtta aggagtgggg gaaaagtagt
    19141 atttttacaa ttgtaaggcc ttgttctgtt tatggccctg gtgaaaggga tattttcagt
    19201 tattttaaac ttgttaaaag cggattatcg ctaactgtcg gatttaagga aaaaaggctt
    19261 tcaataatct atgttgacga tctcgtcaat gccatacttc accttgcttt taatgtaaag
    19321 ccatcaggga caacttattt tgcttccgga gatgaagact gctcttggaa ggaattttcc
    19381 gctaccgtat ccgaagcaat gggtaaaaaa ggtgtaattc cggtaaaact tcctgaattt
    19441 gcaagccttc cagtggcatt agtatctgaa tttattgcta aaatgactga aaaagctgcg
    19501 cttctgagtt tccagaaaat tattgagctc aggcaggatt actggctttg cagtaatgct
    19561 cttctcaaat caacaggctg gaaacctgaa tataccctgc aaaaaggagt cgatgagtcc
    19621 gtcagatggt ataggctcaa tgggtggctc taatcgactt gtttcgttta gtttatacaa
    19681 cattgaaaag aattagattt cagatatatt ggatggatgt ttttgataca gaggtatatt
    19741 tgtgggtgta aaattcggaa catttaaagg cgtttttgtt ccttctacag aagccattct
    19801 cggaacagtg cttttccttc tcatgccggt tcttattgcc gatgtcgggc tgtgggcaat
    19861 gctcggagtc ataattcttg cacacaccgt cacttttgcg acatcgtttt cgattgctga
    19921 ctgtgcgacg aacctcaaca acatcggtcc tgggggaatg tatgcgcttt cgagaaggag
    19981 tcttgggaag gctttcggcg ggagcatagg ttttcagctt tatcttgcgc aggctgcaag
    20041 tatcggattt tattcaatag gttttgccga gcctcttcaa tcgatcattg ctccgtatct
    20101 tgattttata ccaattttta cgcttaccga tcctgcttca atactgatac aaaaacagat
    20161 tctagcttca gtctttttct ttttgttttt tattgtggta atgataggcg ccgattttac
    20221 cctgaaaatt cagacattta ttctttttgc tctcggggca ggaattcttt cggtcatgat
    20281 atctccattt tttggaatta cttttaataa ccttcctgtt ttttcaggca gttttaattt
    20341 ttccggagca agggaaatta cacttgggct ctttttcatg acttttgccc agttttttcc
    20401 ggcagttacc ggaattgacg ccggaatagg aatgagcgga gatcttgaaa atccgagaag
    20461 aagtctggta aagggtactt tttatgccat tggtataact tttgtcatct atcttataat
    20521 agctgtcgtt tatgctatgc ttgataaaaa tataatgatt caaagctata tgaacggagc
    20581 tccggtacct gttctcctga ctgagataat gagctattca tcggcatttc ctgtgaacat
    20641 tctcggcatg tttcttgtga cagggatact ttttgcaaca ggatcatccg ccctttctgt
    20701 tttcatgaca gccccgagaa cacttcagtc tctaaccatt aacggtgttc ttcctgcaaa
    20761 gctgtcgttc ctttccggag attttaaaaa gggaggtaat gagccgagat tcgctgttat
    20821 tgtaacgagt tttatcggtc tttttgtgat atgggcaggc aatataaata cagcctcgat
    20881 gatcgtggga atacttttcc ttgttgttta cgggtggatc aacggcagtg cattccttga
    20941 aagagcgagc gggaatcccg gtttcaggcc gacttccaga aatcactggt ctgtttcact
    21001 gtacggtttt ctcataagtg ttgtgtctat catgcttttt gacccgatga tcggaattct
    21061 cataatcatt tcacagtatt tcatgttcag ggcgatactc agatataaag caggcaacag
    21121 aattgaaggg gtctggtggg gtataatctt ttctcttgcg accaggtggc tgaaaatgct
    21181 tggcaatgtc gttcagggaa gcaagaactg gagacctgtg atgaccgctc tggttatcag
    21241 cgaaaaggga tcttcccctg aaaggacggc atatctcggc tcatttcttg caggacacaa
    21301 agggatggtg aacctcaaca tcataggaaa tgaagcagaa tttaacagtt acggcattcc
    21361 gtattcattt attccaagcg atgatatcaa cgaaacggca atggccctcc tgatgacttc
    21421 acatccgaca ggtgtagaga taaacacagt cctgatcgaa tatgtgaaaa aactccatac
    21481 agcaaaagtt ataactgaaa tgataagtaa aaagaggaac atactccttt tccaaaacag
    21541 ttccaaactc acaaagatcg aaaatatcga catctggtgg agaggagaaa tgaacgggaa
    21601 tctcatggtc cttcttgcct atatcataac tcttgaccgc cgtgagaaat gctgcaatcc
    21661 taagatcaga ataataagaa tggctcaaag caatgagtct gtagacacag acagaattga
    21721 aatggaaaca cttcttgaaa atgccagact taacggtgaa gttgttatac tggaaaatga
    21781 aaacagtgat tttcttgatg tactgaaaaa atattcaagc agtgctgatc tcataatgat
    21841 gggtcttccc ggaaattttg cgaaagagag caaaatgaaa atattcaata taaacgaata
    21901 cttttttgac aagcagatct caaaatatga tgatctgccg ccgatccttt tcataaaaag
    21961 tgctcagtat atagaactga ccgaacaata ggaggaaaca atggaagttt caaaagttaa
    22021 gggactgaca gattcagaac gggagctttt caggtatgat cttgagataa aaaagctcgg
    22081 cagacaggat tttgtctctc cgcttacaca gagcaaaagg atctattcga cacaggttat
    22141 ccctgaaaat gaaaggataa tggctttcat tgacaaaagt gttgtcgatt caatcaaaga
    22201 agttaatatt gatggtcctt catttgaaaa ggcgggtccg agagaaaagc ttttcttcga
    22261 gcctggtgaa acagtttccg ctgttgtgac atgcggaggg atatgcccgg ggctcaatgc
    22321 tgttatccga ggtatcgttg tgatgaacta ttaccgttac aacaaccaga gaattctcgg
    22381 aataagcaac ggttatgcag gtcttgtgaa ggaatacggt tatgaag
//
LOCUS       DAOOYB010000010        21820 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_2245_length_21820_cov_10.792741, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000010 DAOOYB010000000
VERSION     DAOOYB010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 21820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21820
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2245_length_21820_cov_10.792741"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1289
                     /locus_tag="PL056_01275"
     CDS             <1..1289
                     /locus_tag="PL056_01275"
                     /inference="COORDINATES: protein motif:HMM:NF012348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HPM45676.1"
                     /translation="QMLKESYWKRTKGQIRTCLEESLEMLTYYGFEPEMGHKEVGGVK
                     GKISSNGRLFDVMEQLEINWKFKDPVTACDNELWARIVVKEVFRRAGLEVTVIAKPVE
                     GVAGSGEHMHTGAVIHLKNGSKFNLFAPSDEKLFLSSAGYGALMGILKNWEVVNPFVS
                     HSNSALKRLKPGFEAPVSIAASLGMSPVSPSRNRSVLACLVRGASPMSVRFEIRAPNP
                     HTNSYLAMSAFLIAMLDGMKYAGGKNAADLDAEIHKKSGESNSYLATDREYVCEHDLF
                     EKYNASEREQLFGKTPATVFEVISSIENTPELYKQTPLSPLIVRSFYLSALRKWEVEL
                     LQREIPNIIKELRSVKRFVEHENDLDQKTWESIESLRNLIAKDGLGNKSLVSRLKESF
                     SKQDYAKAGSTFLEIQRVFAEFKEVTNLYRNNIISM"
     gene            complement(1340..4936)
                     /gene="nifJ"
                     /locus_tag="PL056_01280"
     CDS             complement(1340..4936)
                     /gene="nifJ"
                     /locus_tag="PL056_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HPM45677.1"
                     /translation="MPKKKVVMKCIDGNHAATHSSYALTEVAAIYPITPSSNMGENAD
                     EWSAYGLKNIFGEKVLVTELQSEGGASGAVHGSLSAGALTTTYTASQGLLLMIPNLYK
                     MAGELLPGVLHVSARAVAAHALSIFGDHSDVMACRQTGVGLISSNSVQESMDLGLATH
                     IAAIRSSLPFIHFFDGFRTSHEISKVEMIDYEDIKKIAPYEEIAAFKENAMRPDRPVL
                     KGTAQNPDIFFQAKEASNPYYDRAPGIVQEAFDRVFSITGRKYHLFDYYGHAEAERVI
                     IAMGSGCETIEETINYLLEKGEKVGLVKVRLYRPFSAEHFMRALPATTKSIAVLDRTK
                     EPGSEGEPLYKDVSTLLYERKETIKVIAGRYGLSSKEFTPGMVKAIYDNLKAREPKNF
                     FTVGINDDVTFTSLGYEPLDTVVKGTVQCKLYGLGSDGTVGASKNAIKIIGEETDMYA
                     QGYFSYDSKKSGGITVSHLRFGKERIQSPYLITDADYVACHNQAYVYQYDLAKTLKKD
                     GAFVLNTVWDEKETEENLPASLKKDIARKNGKVYIIDAVKIADRLGLGQRINMIMLTV
                     FFKLAKVIPFEDAVKDLKKANEKSYGKKGKAVVDMNNAAIDAAAAELKEIKYDCEKWL
                     HAEVKEIDIKKVHEQFVDEAEKIMVEGIINPIARLNGDDLPVSAFAYEERGMLPGPDG
                     SFPTATTRFEKRGVAIKLPKWIAENCIQCNKCSYVCPHAAIRPVLVTKEELEEAPETF
                     KTIKAVGKELANLEYRMQVYPMDCQGCGNCADICPAPKGKALDMVFFNEIVEVEAKNN
                     DFSINVPVRDDLMPKTTVKGSQFQQPLFEFSGACAGCGETPYVKLLTQLFGDRMFVSN
                     ATGCSSIYGGTAPTIPYCKNKDGYGPTWANSLFEDNAEYGYGMALAVRQKRGRIEMLM
                     NEILDSKCDCSKNVSNELKALFKEWIANKNDADKTKEISPKLISALANEPVECKTLDQ
                     IFELSEYFVKKSVWVLGGDGWAYDIGYGGLDHVLASGENINVLVLDTEVYSNTGGQAS
                     KASQFGQIAKFAASGKKMRKKDLGMISMTYGYIYVASVAMGADHNQVLKAMLEAEAYD
                     GPSIIIAYSPCINHGIDMTRSQNEMKLAVETGYWPLYRFNPALREEGKNPFQLDCKEP
                     TKDPQEIINNEVRFKALDKQFPDEAVKLQALLKADLKARWERLKKLSENTIL"
     gene            complement(4961..5545)
                     /gene="recR"
                     /locus_tag="PL056_01285"
     CDS             complement(4961..5545)
                     /gene="recR"
                     /locus_tag="PL056_01285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HPM45678.1"
                     /translation="MIHPEVKQLIEELKKLPGISLKLAQKLAYSILNDAELKKTLGNI
                     VKLSENIEVCHDCGFFRTEGSSCVNCSGKSSEICVVATYSDMSAIERTGCFSGSYHIL
                     GGLIAPLENTLPEDLLIDQLKKRVEQNPGVEILLALPFSIEGDATALYIKDELDGLNM
                     KLSRIARGLPAGCAPDVLDRKTVLEAFSGKTYIN"
     gene            complement(5552..5863)
                     /locus_tag="PL056_01290"
     CDS             complement(5552..5863)
                     /locus_tag="PL056_01290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HPM45679.1"
                     /translation="MNIQNLMQQAQKMQKKMASLDAEFKNEIFKTETGGGAVTITMNG
                     DMKIIEVKMSDELIKSQDHEMIADLFTAAVNQSIDTVKKEKETRLSKVVGKMGGMPGL
                     F"
     gene            complement(6027..6341)
                     /locus_tag="PL056_01295"
     CDS             complement(6027..6341)
                     /locus_tag="PL056_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45680.1"
                     /translation="MKKHLFWIYIAILEINMVISMLLQHLPKMMGYITNKEGLKVLAM
                     YDGCFKVSIIVMFLMFEVFFIASFFIDELAAKRKRAAIIFLINLMIVSHLFMFPLVFF
                     NG"
     gene            complement(6822..7793)
                     /locus_tag="PL056_01300"
     CDS             complement(6822..7793)
                     /locus_tag="PL056_01300"
                     /inference="COORDINATES: protein motif:HMM:TIGR01784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HPM45681.1"
                     /translation="MSKNEPVHNNLFGKTFQSIENTNDFIKGALPAGIVRKLDFSTSV
                     IQSTSHVDDSLRSHLSDFVIKIKTKDDENVDLYFLFEHKSHKDKNILWQLLKYQYLML
                     EEDYKAERKFRIIIPVVFYHGHGKWNLKRSFSESFNVPHYFRKHTLNFEYFLYDTKEF
                     DLSQGDRFSNNVFLMSVLSLMKQYGKVDAEKFRTVVRYMKSSGMDWKDDQTLVLIRYF
                     VMTNEVDKDKVMTIITEEFGPDAEEMMPSLGKKIYNEGRQEGREEGIQIGIEKGIEKG
                     IEKGAFAKAVETCRNALRSGILPELVAKITNLPLEKVLEIQKTLQTD"
     gene            complement(7971..8909)
                     /locus_tag="PL056_01305"
     CDS             complement(7971..8909)
                     /locus_tag="PL056_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45682.1"
                     /translation="MKTIIIILIIFFMGVIYSEENTKTPAFKNSEENPIKESEKKGEK
                     KKVSTAEKVFFCAKGYKGPAFLGWCKDGNYYGPVHMGSIGVAVADGFYGLVQISGFLS
                     LSDNFYGLVQIGGFGVGSKKIFYGFGQIGGAVATSEIFNGVFQFGGLFSVAYKKFNGL
                     VQIGGLITGADKFHGFISLSSIMSGISNFEGGFQISLFSMSGFNFLGPFLSTEKQEKF
                     LKDKKIHGFYGGFQIGVLVSGASTFKGVSQIGAYNVSLQHYGGQIGIVNRSKVVRGVQ
                     IGVVNYTEKLYGLQLGLVNIAQNGFLPYTTILNIGW"
     gene            complement(8924..10102)
                     /locus_tag="PL056_01310"
     CDS             complement(8924..10102)
                     /locus_tag="PL056_01310"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HPM45683.1"
                     /translation="MENHIAIEVFTGTGSDTYFFLTDERGNVVALTDEDGTILERYRY
                     RVYGEHEVLDDLFEEKDCGVDTCYAGNVHNFLWGGSLYEPETDLYWMRNRYYHKDMHR
                     FINQDPIGIWGDANNLGNGFAYVAGMVIEASDPTGLGPVGDWFKALFNAIVNWFKTGV
                     WSFNYNSSESAPSPESEAEKAEQERQAAEKQKAAEALNQDKTDADKAAEEDGKPEETD
                     SQKTEEKIKELLKKGIAALLINALSPWGPKADGEDGKGPEGEKGWTFMVSIDLFKLFS
                     KYPSDPYVDQTDVEKMIFYRMLVMKLLKKGEEMPERIIIDDRSDTGRHGVRFFDNPYF
                     RQKDPLTEILYLERDDAGKIYFNRFGKNGIYGGSERKNGYETVTEKGFNRGDIDPYFY
                     "
     gene            10234..12006
                     /locus_tag="PL056_01315"
     CDS             10234..12006
                     /locus_tag="PL056_01315"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019119380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HPM45684.1"
                     /translation="MQHEMRWRLDDLFPGFDSKMFSASCSDIDKAIEDYDNWADGLKK
                     RKDPVEKIIEEYIAKRNSAGLALSRLSDFAELSFSVNTADQEAVKFMELFEKKLSQIE
                     VIDVKFVKWIVKHKHADLLNGSESLKNHSFHLSEMIRKGKYMLDSSKEKIISDMKATG
                     SSAWTNLHNVLTSSLLVDFTENGKIAEQKTLPQVRSMAYSPDPLTRKKAYEAELASYP
                     KIEKSICACINGIKGEVITTSRLRGYKSPLEMTLLRSRMDKRTLDAMMRSLKKYLPVF
                     RKYFRKKALMLRHRNGLPFFDIFAPISNTDMQFSYSQAKDFIVEKFSSFSADLGLFAK
                     NAFENGWIDAQPREGKVGGAFCANLRSINQSRILSNFSGSYNDVRTLAHELGHAFHGH
                     CLNNESFLNSNYPMPLAETASIFCETIVNHAALKESTPDQQLSILEAEISSAGQVIVD
                     IYSRYLFETEVFENRASSSLSVDQLKEIMLSAQKEAYAEGLDPEYLHPYMWLVKPHYY
                     VADYNFYNFPYAFGLLFAKGLYAIYLERGSSFVEDYKRLLSETGRKSILDVGKSVGID
                     LNNTSFWNGSFKVIKEDIGRFLKY"
     gene            complement(12008..12970)
                     /locus_tag="PL056_01320"
     CDS             complement(12008..12970)
                     /locus_tag="PL056_01320"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HPM45685.1"
                     /translation="MKKRIILTSELPGERYKKLLNESGVEVKVLSPEERKDLRNAVSS
                     FDPEGLITLLSDKIDKSVITAAPRLKVISNYAVGFNNIDVKYCSEKGITVTNTPDVLT
                     DSTADVAILLLLTASRRSYEGEKFTREGKFTGWEPDLFLGKSLKGKTFGVIGMGRIGR
                     ATAKRAKAFGLKIVYWNRTRISIDKETELSAEYCEIDDLIRNSHFISLHLPFVPELHH
                     LIGKKEIASMRSDAILINTARGALVDEDALADALIEKRIYAAGFDVYEYEPVINQKLF
                     ALNNAVLLPHIGSAAEETRAEMAEMTINGCLSVLEGKKPANSVN"
     gene            complement(12980..13966)
                     /locus_tag="PL056_01325"
     CDS             complement(12980..13966)
                     /locus_tag="PL056_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45686.1"
                     /translation="MKTKLFIIFIVAGSVIMNIYYFTKDEKCVEDSVSTEVFIYPADH
                     SSIDFELSYDPENPQYMNFSLEGDNIIFANFYLKGSMIDSYRRKYDKRLSDKLIRNIQ
                     EIVYFLNDYKIWFNERDRVTLFYNEQNEKIVYLRFKNAVRKTLSDVYLFETIDGERYV
                     NSDGSYLQPCIVNGPFDGCPDVRFINESNTLVPVFNVRMHHNVKLPFLAKLVNVDHSR
                     NSGGEMEFIYSNYATRAFFKGLGHINDKLKKNSLYKKDAVIGKSGFVLQDGSGGVVYF
                     LRKNENTPVSPFLFHHIERNFLPQKYDRNFQIVKSFYARQLEFGLKFEQQYY"
     gene            complement(13956..15011)
                     /locus_tag="PL056_01330"
     CDS             complement(13956..15011)
                     /locus_tag="PL056_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HPM45687.1"
                     /translation="MDNIRKHIEEIEKRELSWAACLSSASKGRVKYEELCSIRPAFQH
                     DRDRIVHSKAFRRLGDKTQVFLSPKGSHYSNRLTHTLEVTQIGRTIAKALFLNESLTE
                     AIGLGHDLGHTPFGHSGERALGKLLKNGFRHEKQSIRVVDFLARNGQGLNLTYEVRNG
                     ILMHSKGKGPLLKKDALPETLEGQIIRLADIIAYLNHDIDDAVRGGFLDIKKVPHNDI
                     LGEKGSQRINYMVTDLISKSFEAIEAKEKIIRFSSETTKMIEDLRDFMFSNIYEMPEI
                     RREFEKSEKIIKSLFYYFKENYDNFTEFYSNPVSDDPETNITDFIASLTDSYAIHLYT
                     KLFIPERLKFPDFKNED"
     gene            complement(15004..16644)
                     /locus_tag="PL056_01335"
     CDS             complement(15004..16644)
                     /locus_tag="PL056_01335"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HPM45688.1"
                     /translation="MAAFSRLKRFSEIGAVAAKYGLTPFIPRTLLSVFKLENRNAEEK
                     SFKDMKLEERFRLAFEELGATFIKMGQILSLRGDIIPISMAEEFEKLTDNVNPMDFAL
                     IKPIIEEELAASLEEIFDNFNETPIAAASISQVYSAILKETGESVVIKVRKPGVVETI
                     KSDMEIIIWIADILQKTSSYSKNINFKGIAEEFFWTMNQELDLVIEKSNTQKLASNFD
                     GEQWEWLSFPKMNEKYCTGRILVMERIDGVKLDDVIECTDQAMFDRKIIADRGAKALM
                     KMVLLDGFFHGDLHSGNIFFKPGNHIVAIDCGMCCRIDRYLREKIADMFIAFIARDFE
                     KVANIYIDMSESGTPVNKKEFISDIRKVFDSLPDNLSEINTTEIVKKSFNIMFKHKLK
                     IPRELTQLIKSFSLIEGLCRELDPEFELMTVAEKLSKELFAERYSPDRIAGDVFSLLI
                     KMIDLLKNLPGVLSNIAEKIEDGTIQHRFLVLFGRSERSFISRMVTRICSAFIITGSM
                     MAAGSVDNEFYLFVIYALFGVSVIVLILTFYKGKDRDG"
     gene            complement(16662..19208)
                     /locus_tag="PL056_01340"
     CDS             complement(16662..19208)
                     /locus_tag="PL056_01340"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HPM45689.1"
                     /translation="MCGVVSIVYRKNIANLGNEAASLLKKLEYRGYDSTGGAFIKEDG
                     TVTLRKKVGAPSRVIEQLEIPKQSGFKFIGQVRWATYGAVTDVNAQPHEVNCLVHMVG
                     AHNGNVSNTDKLKDFLADNGHTVLSDNDGEMVVHLVEHFYSALMHKCKPSTNDEKIAL
                     MIRAIRKAQKMIEGSYAACVTLPEIPGVFAIKAGSSLYAGKGSDNAGNFIVVSSDLTS
                     ILSKTRFLIPMSEGEGIYFDNDSYKLFSLTEDKEWVTKLSRSRLNIADIQLQPKFHFF
                     MEQEIFSSPRNIDNLMLYYFNDESEKELHAIFEKNKDTAREILYSLLALYDVYDSNEL
                     KKEFENIRKLKTFKTIFAKASKYCADPKHFVFKSEETTLLEELLGINEKYFTELWLID
                     SMLVWKKKRMIIKYRDKLVNAFKESGKRGGRIYFVASGTSYHAALTGAYFFNNLTDIS
                     IIPANPGGFRSGFINSLRENDIVVGVSQSGETKDLVDIFNDIRKVCSDKVQLLTIVNN
                     ENSTLPQEKSDFYLPIMCGSEIAVAATKSFISQIALFYILAALVKNEEDDVFNDLKKI
                     RYFMDFTLKNIDIPVSDTALKLFMKPSIHILGTSLTGLAKEGALKIREVVLNHTEGYD
                     AAEFKHGPNTILGKNTIYSLTDMELLLGNMIEMFEQLFDKGFLKGNGAKADMVREYTN
                     TVKNLKFRKVDDDTVIKPFTSKEDRNAMKKMYSLFAEKTGIENFFSNYPLVFVCPPDE
                     RDKRITISQIHTHKIRGADIVLISEDDDDLKKAATGKPASTGKYWSKFIELPRTGDRN
                     IFIFEAAVVLQLLAFKMSVAKMKYLNRSQIENHGVHPDVPKNVSKSITVD"
     gene            complement(19233..21014)
                     /locus_tag="PL056_01345"
     CDS             complement(19233..21014)
                     /locus_tag="PL056_01345"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HPM45690.1"
                     /translation="MTIVKRLEQLRMMMKKVGIDAYIIPSADPHQSEYVAPRWKTREW
                     ISGFNGSAGTVVVTMKKAGLWTDGRYFLQAEEQLKNSTITLFKAGLPETPDYKEWLTK
                     ELKKGANIGFDSSVMSINEVDDLKRSFESKGLKVKPGKDLVDAIWKDRPAFPSARAVL
                     HPIKYAGTGRPDKFKMIREKMAQSGVDYHLFASLDDIAWIFNIRGNDVSCNPVVMGFG
                     LIGKKSAELFVKNGKFNAKDVKTLEKDGVKLRDYDKIYKVLEKLGKGKLFLDPARCSQ
                     SLLDAVDTKKVEVVKGMNMSQIMKAVKNATEIEGIKKAHITDGVAMVKFLCWLDKTLG
                     REKITEISASDKLETFRAAGENFAGLSFDTISGYGEHGAIIHYSATKESSIEVKPEGL
                     YLVDSGGQYLTGTTDITRTVVVGKLTKKMKKDFTNVLIGHINVSTAKFPQGFTAGTHL
                     DALARIALWKNGQNYNHGTGHGVGAYLSVHEGPMGISPRWLETKLVPGMILSNEPGYY
                     EAGEYGIRIENLVLVKEIEETKWGKFLGFENITMCPIDIRAIEPGMLNAEEKKWLNDY
                     HKDVFRNLSPQLGKEEKEWLKKMTASI"
     gene            complement(21290..21595)
                     /locus_tag="PL056_01350"
     CDS             complement(21290..21595)
                     /locus_tag="PL056_01350"
                     /inference="COORDINATES: protein motif:HMM:NF025120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPM45691.1"
                     /translation="MRNKGYKTESLDKTIERFVDKNSAKEQILNEMYEEMKLGIIMDN
                     LRELRKKKGLTLKEVAELMETSESAMSRLEKQIKNVTISTLARYAKVLGRKIEIRVV"
BASE COUNT         5949 a         4815 c         4164 g         6892 t
ORIGIN      
        1 gccagatgct taaggagtct tactggaaaa ggacaaaagg acagataaga acatgtcttg
       61 aagaatctct tgaaatgctt acttattatg gttttgagcc ggaaatgggg cacaaagagg
      121 tcggtggagt taaaggaaag atctcgtcaa acggccggct ttttgatgta atggaacagc
      181 ttgagataaa ctggaaattc aaagatccgg tgactgcgtg tgacaacgag ctctgggcaa
      241 gaatagttgt aaaagaggtt ttcagaaggg cagggcttga ggttacagtt atagcaaaac
      301 cggtcgaagg tgttgccgga agcggagagc acatgcacac cggagcagtg atccatctga
      361 aaaatggcag taaattcaac ttgttcgcgc cttcagacga aaagcttttc ctttcaagtg
      421 ccggttacgg tgcgttgatg ggtatcttaa agaactggga agttgtgaat cctttcgtat
      481 cccactccaa ttcagctctt aaacggctga aaccggggtt tgaggcacct gtatccatcg
      541 ccgcatctct tggaatgtcc cctgtcagtc cttcaaggaa caggtcggtt ctcgcatgcc
      601 tggtaagagg ggcgagtccc atgtctgtcc gttttgagat aagggctccc aatccccata
      661 caaattcata tctggcaatg agtgcgtttc tgatagcaat gcttgacgga atgaaatatg
      721 ccggtgggaa aaatgccgcc gatcttgatg ctgaaattca caaaaagtcc ggagaatcga
      781 acagttatct tgcaacagac cgggaatatg tgtgtgagca cgatcttttt gaaaaataca
      841 atgcttcaga aagagaacag ctttttggaa aaacacccgc aacagttttt gaagttataa
      901 gttcaataga gaacactcct gagctttata aacagacacc gttgtcaccg ctgatagtaa
      961 gatcttttta tctcagtgcg ctgagaaagt gggaggttga actgctccag agagaaattc
     1021 ccaatataat taaagaattg agaagtgtta aaaggtttgt tgaacacgaa aatgatctcg
     1081 atcagaaaac atgggagagc atagaaagtt taagaaacct tatagcaaag gatggactcg
     1141 gcaacaaatc gcttgtgtca agacttaaag agtcattttc aaaacaggat tatgcaaagg
     1201 caggttccac atttcttgaa atacagagag tttttgcaga atttaaagaa gttacgaatc
     1261 tttacagaaa taacatcata tcaatgtgat aagtaagggc gaaaaatctt tcgcccctac
     1321 cgtaatttat tgaaaaatct taaagtattg tattctcaga aagttttttg agccgttccc
     1381 atcttgcttt gaggtctgct ttaaggaggg cctgaagttt taccgcttca tccgggaact
     1441 gtttgtcaag agctttgaaa cggacttcgt tattgatgat ctcctgagga tcttttgtcg
     1501 gttccttgca gtcaagctgg aacggattct tcccttcttc tcttaaagca gggttgaatc
     1561 tgtaaagagg ccagtaaccg gtctcaacag caagtttcat ttcgttctgg ctgcgagtca
     1621 tgtcgattcc gtgattgatg catggagagt aggcaataat tattgacggg ccgtcataag
     1681 cttctgcttc aagcattgcc ttgagaacct gattgtgatc tgcacccatt gcaactgaag
     1741 caacatagat gtatccataa gtcattgaaa tcattccgag atctttcttt ctcatctttt
     1801 tgccgcttgc tgcgaatttt gcgatctgac cgaactggga cgcttttgat gcctgtccgc
     1861 cggtgttgga ataaacttcg gtatcaagaa cgaggacatt gatgttctca ccgcttgcaa
     1921 gaacatggtc aagtccgccg taaccgatgt cataagccca tccgtctccg ccgagaaccc
     1981 agacagattt tttaacaaaa tattcgctga gttcaaatat ctggtcaagt gttttgcatt
     2041 cgaccggttc atttgcaaga gcagaaataa gcttcggaga tatctctttt gttttgtctg
     2101 catcgttttt attggcgatc cactctttga aaagtgcttt gagttcgtta cttacatttt
     2161 tactgcagtc acattttgaa tcgagtattt cgttcatgag catttcgatc ctgcctcttt
     2221 tctggcggac tgcaagagcc atgccgtaac cgtattcagc gttgtcttca aaaagggagt
     2281 ttgcccatgt cggtccgtat ccgtccttgt ttttgcagta aggaattgta ggagcggttc
     2341 cgccgtagat agatgaacag ccggtagcat tggaaacgaa cattctgtct ccgaaaagct
     2401 gtgtcagaag tttcacataa ggagtttcac cgcaacctgc acatgctcct gagaactcga
     2461 aaaggggttg ctggaactga cttcctttca cagtggtttt gggcataagg tcatcacgaa
     2521 ccggaacatt aattgaaaaa tcgttgtttt tcgcttcaac ttcaacgatc tcgttaaaga
     2581 aaaccatgtc aagggctttc cctttcggag ccggacagat gtcagcacag tttccgcaac
     2641 cctgacagtc cataggatat acctgcattc tgtattcaag gtttgcaagt tctttgccta
     2701 ctgccttgat cgttttgaat gtttcgggag cttcttcaag ctcttctttt gttacaagca
     2761 caggcctgat ggcggcatga gggcatacat aagaacattt attgcactgg atgcagttct
     2821 ctgcgatcca tttcggaagt ttaattgcaa caccgcgctt ttcaaatctt gttgtcgctg
     2881 tcgggaagga accgtcggga cccggaagca ttcctctttc ttcataagca aacgcactca
     2941 ctgggaggtc atcgccgttg agccgtgcta tcggattgat aattccttca accattattt
     3001 tttcagcttc gtcaacgaac tgttcgtgaa ctttcttgat gtcgatctct ttgacttcag
     3061 catggagcca tttttcacag tcatatttga tctctttaag ttcagccgcc gccgcatcga
     3121 ttgccgcatt attcatgtca acaacagctt tgcccttttt accgtaagat ttttcatttg
     3181 cctttttgag gtcttttacg gcatcttcaa aaggaatgac tttggcaagt ttgaagaaaa
     3241 cagtgagcat tatcatgttg atacgctgtc caagaccgag tctgtcagca attttaacag
     3301 catcaattat ataaacctta ccgttctttc ttgcaatatc ctttttaagt gaagcaggaa
     3361 ggttttcttc agtttctttt tcgtcccaga ctgtattaag aacgaacgca ccgtcctttt
     3421 taagtgtctt tgcaagatcg tactgatata catatgcctg attgtggcat gctacataat
     3481 cagcatcagt gataagatat ggtgactgaa ttctttcttt gccgaatctc aggtgactga
     3541 ctgttatgcc gcccgatttc tttgagtcgt atgagaagta accctgtgca tacatatcag
     3601 tttcttcacc gatgatcttg atcgcattct ttgatgcacc gaccgttccg tctgaaccga
     3661 gtccgtaaag tttgcactga accgtccctt taacaacggt gtcaagcggt tcatatccca
     3721 gtgatgtgaa tgttacatca tcattgatcc cgactgtgaa aaaattcttc ggttcacggg
     3781 ctttaaggtt gtcgtatatc gcttttacca tgcccggagt aaactctttt gatgaaagac
     3841 cgtagcggcc ggcaatgact ttgattgtct cttttctttc ataaagaagc gtggaaacat
     3901 ccttgtaaag gggctcgcct tcgcttcccg gctcttttgt tctgtcaagg acagcaattg
     3961 actttgtcgt tgcaggaaga gctctcataa agtgttcagc actgaaaggt ctgtaaagcc
     4021 gcactttcac aagaccgacc ttttctcctt tttcaagaag gtagtttatt gtttcttcaa
     4081 tggtttcaca tccgctaccc atcgctatta ttactctttc agcttctgca tgaccgtaat
     4141 aatcaaaaag atgatacttt ctgccggtta tgctgaaaac tctgtcaaat gcctcctgaa
     4201 ctattcctgg agctctgtcg taatatggat ttgatgcctc tttcgcctgg aagaagatgt
     4261 cagggttctg tgctgttccc ttgagtacag gcctgtcggg gcgcatcgca ttctctttaa
     4321 aagcggcgat ctcttcgtag ggggctattt ttttgatgtc ttcgtagtcg atcatttcaa
     4381 cttttgaaat ttcgtgactt gttctgaatc cgtcaaagaa atgaatgaaa gggagacttg
     4441 aacggattgc ggcgatgtgt gtcgcaagac caaggtccat tgattcctga accgagttac
     4501 ttgaaataag cccgacacct gtctgacggc atgccattac atcgctgtga tctccgaata
     4561 ttgaaagggc atgagccgca acagctcttg cgctgacatg aagaacgccg ggaaggagct
     4621 ctcctgccat tttgtaaaga ttcgggatca tcagaagaag tccctgtgaa gcagtgtatg
     4681 tcgtcgtgag tgcgcctgca cttaatgaac cgtgaactgc tccgcttgct ccgccttcag
     4741 actgaagttc agttacaaga actttttcac cgaaaatatt tttgaggccg taagcgctcc
     4801 attcatcggc attttcgccc atgttggagc ttggggtgat aggatagata gcagcaactt
     4861 cggttaaagc ataactcgaa tgggttgccg cgtggtttcc gtcgatacat ttcataacaa
     4921 ctttcttctt tggcattttt tcctccgtat aagcaaagtt tcagttaata tatgtcttcc
     4981 cgctgaatgc ctcaagaaca gtctttctgt caaggacatc gggagcgcag ccggctggaa
     5041 gtcctcttgc aatgcgggac agtttcatgt taagtccgtc aagttcatct tttatgtaaa
     5101 gggctgttgc gtctccttca attgagaagg gaagcgcaag cagtatttca acccccggat
     5161 tctgttcaac tcttttttta agctggtcta tcagcaaatc ttcaggcaga gtattttcaa
     5221 gaggggcaat caagcccccg agaatgtggt aagaacccga aaaacagccc gttctttcaa
     5281 ttgcgctcat atcggaatat gtcgccacga cacatatttc tgaacttttt cctgaacagt
     5341 tcacacaact tgaaccctca gttctgaaaa agccgcagtc atgacacact tctatatttt
     5401 ccgaaagctt cacaatattt ccaagtgttt ttttaagttc agcgtcattt aatatcgaat
     5461 atgcgagctt ctgtgcaagt ttgagcgata ttccggggag ttttttcaac tcctcgatca
     5521 actgttttac ttcaggatgg atcatttcac ctcagaaaag acctggcatt ccgcccattt
     5581 ttccaacaac ttttgaaagc cgggtctcct tttccttttt aacggtatcg atgctctgat
     5641 taactgcggc tgtaaaaaga tctgcaatca tttcatggtc ttgtgatttt atgagttcat
     5701 ctgacatttt cacttcgata attttcatat caccgttcat tgttattgtg acagctccgc
     5761 caccggtttc agtcttgaat atctcgtttt tgaactcagc atcaagagaa gccattttct
     5821 tctgcatctt ctgtgcctgc tgcataagat tctgaatgtt catttccgcc tcatttcaga
     5881 aattatataa aaaccaacaa cgacaataac atattaaggg aaaacaaaaa tgcaaggata
     5941 ttcaaatcgg gacattcttt cctttcgctg ttttctgtat ccataaatac ttcattgtgg
     6001 gcctcccttg ggggttattt aaaaacttaa ccattaaaaa acactaaagg aaacataaaa
     6061 agatgtgaaa caatcatcag attaatcagg aatattattg ctgccctctt tctctttgcg
     6121 gcaagttcat caataaagaa actcgcgata aaaaaaactt caaacatcag aaacattacg
     6181 attatactca ccttaaagca tccatcatac attgccaaca ctttcaatcc ttccttgttt
     6241 gttatataac ccatcatctt tggaaggtgt tggagcagca tggagataac catgtttatt
     6301 tcaagaatcg ctatatatat ccagaaaaga tgttttttca ttttgaactc ctgtttactc
     6361 aaaatgtgca aaaagaacca gacacataat tattaatatc agacgaaata gaaaaaacat
     6421 gactgtgttt tttcgcataa ttttaatttt ggaatgaaaa aaactactta taaaaaatat
     6481 cacatgaact aaaaacatca ttataaagga cgctattaca caaataactg ctaaacctga
     6541 tcgtccaact gaatctatac tatatcctaa aatcaatcca gcaattagat taaaataact
     6601 aatttcttcg acgaaaagta gcgaataaaa tagcttgtta tctttaattt tatcagctat
     6661 tactcccatg gatctacctc cggctgaatt attcctctca tttgccacca aactctgtac
     6721 ctgtaacgct caagaatatt accagaatta tctgtcaatg caacaacatt gcctcgttca
     6781 attatatctt accggttaat tttgtgttct ataatgaaaa tttaatctgt ctggagagtt
     6841 ttctgaattt caagaacttt ttcaagtgga agattcgtta ttttggcaac cagttcaggc
     6901 aatatccctg atctaagagc attcctgcaa gtttcaacag ctttggcaaa agctcctttc
     6961 tcaattcctt tctcaattcc tttctcaatt ccgatctgga ttccttcttc tctaccttcc
     7021 tgtctgcctt cgttgtatat tttctttcca agtgacggca tcatttcctc cgcatcaggt
     7081 ccaaattcct ctgttattat agtcattact ttatctttat caacttcatt cgtcattaca
     7141 aaatacctta tcagaacaag tgtctgatca tctttccagt ccattccgct gcttttcata
     7201 tatctcacaa ctgtcctgaa tttctccgca tccacttttc cgtactgctt catcagcgac
     7261 agaacgctca tcagaaacac attgttgctg aatctatccc cttgtgaaag gtcaaactcc
     7321 tttgtgtcgt aaagaaaata ttcaaagttg agcgtgtgtt tcctgaaata atgcgggaca
     7381 ttgaaagatt cagaaaaact tctcttcaag ttccattttc cgtgaccgtg atagaaaacc
     7441 acagggatta ttatcctgaa ctttctttcc gctttataat cctcttcaag catgagatac
     7501 tgatatttca gcaactgcca gagaatgttc ttatctttat gcgacttatg ttcaaaaagg
     7561 aaatagagat caacattttc atcatcttta gtttttatct taatgacaaa atcgctcagg
     7621 tgacttctta atgaatcatc gacatggctt gttgactgga tcactgatgt tgaaaaatca
     7681 agctttctca cgatccctgc cggcagagca ccttttatga aatcgttagt gttttcaata
     7741 ctctggaagg ttttgccaaa caaattgttg tgcaccggct cattcttgct cataaatatc
     7801 cccgaattag aaaaatcaga aagctaatgc acgataaatt gcgtggacga attagtcaag
     7861 aaaataatca ggctgtttct gcaaaaactg gaacaaacat aaaagctgca atcaagatcc
     7921 aaaaatactt catgatgggt ctccttgggg gttatttttt ataaagtagc ctaccacccg
     7981 atgttcaaaa tggttgtata cggcagaaat ccgttctgtg caatgttaac gagtccaagc
     8041 tgtagtccat aaagcttttc ggtataattt acaactccga tctgaacacc ccggacaact
     8101 ttcgatctgt tcactatgcc tatctgacca ccgtaatgct gtaatgaaac attgtaagct
     8161 ccaatctgag aaacaccttt aaatgtcgat gctccgctca caagcacacc aatttgaaat
     8221 ccaccgtaaa aaccgtgaat ttttttatct tttaaaaact tttcttgttt ctctgtggac
     8281 aaaaaaggtc ctaaaaaatt aaatcctgac atagaaaaaa ggcttatttg aaagccacct
     8341 tcaaaatttg atattcctga cataatgcta cttaaagaaa taaatccatg aaatttatct
     8401 gcacctgtaa tcagtccgcc aatctggaca agtccgttga attttttata tgcaactgaa
     8461 aaaagtccgc caaattgaaa aactccgtta aaaatttctg aagtagcaac ggcaccacct
     8521 atctgtccaa aaccataaaa tattttttta gatcccacac caaaaccacc tatttgaacc
     8581 aatccataaa aattatctga caacgataag aaaccactga tctgaaccaa tccgtaaaac
     8641 ccatctgcaa ctgccacacc aatacttccc atatgaaccg gtccatagta attaccatct
     8701 ttacaccagc caagaaatgc cggaccttta tatccttttg cacagaaaaa cactttttca
     8761 gccgtgctga ccttcttttt ttcccctttt ttttcacttt cttttatggg gttttcttca
     8821 ctatttttaa aagcaggagt ttttgtgttt tcttcagaat aaatcacccc catgaaaaat
     8881 atgatcagaa ttataattat agttttcaca atgaccctcc caatcaatag aagtatggat
     8941 ctatatctcc cctgttaaac cctttttcag tgacagtttc atacccgttc ttcctctcgc
     9001 ttcctccata aattccgttt tttccgaaac gattgaaata aatttttcct gcgtcatcgc
     9061 gttcaagata aagaatttca gttaacggat ctttctgtct aaaataggga ttatcaaaaa
     9121 accgtactcc gtgtctgccg gtgtcactgc gatcatcaat aataattctt tccggcattt
     9181 cttctccttt tttcagcaac ttcataacta gcatcctata gaaaatcatt ttttccacat
     9241 ctgtctggtc tacataagga tctgaaggat atttcgaaaa caatttaaat aaatcaatcg
     9301 aaaccataaa agtccaccct ttttctcctt ctggaccttt cccgtcttca ccatctgctt
     9361 ttggccccca aggagacaaa gcattgatta ataaagcagc tatacctttt ttaagcaatt
     9421 cttttatttt ttcctctgtc ttttgagaat ctgtttcttc gggctttcca tcttcttcag
     9481 cggctttatc tgcatcagtt ttatcttgat ttaatgcctc ggcggctttt tgtttttccg
     9541 cagcctgccg ttcctgttct gctttttctg cctcggattc gggacttgga gcggattcac
     9601 ttgaattgta gttaaagctc cataccccag ttttaaacca atttacaatt gcattaaaaa
     9661 gagctttaaa ccaatcccca acaggaccca acccagtagg atcacttgct tcaatcacca
     9721 ttcctgcgac ataagcgaat ccgttgccaa gattattcgc atctccccag attccgatgg
     9781 ggtcctgatt gatgaagcgg tgcatgtctt tgtggtagta tctgtttctc atccagtaga
     9841 ggtcggtttc cggttcatag agtgagccgc cccagaggaa gttgtggaca tttccggcgt
     9901 agcaggtgtc aacaccgcaa tctttttctt caaacagatc atcaagcact tcatgttctc
     9961 catagactct gtacctgtaa cgctcaagta ttgtaccatc ttcatctgtc aatgcaacta
    10021 catttcctct ttcatcggtg aggaaaaaat aagtatcaga gcccgtacct gtgaaaactt
    10081 caatggctat gtgattttcc atacccgaat caatgacatt ggtgtaattc cccgggtcaa
    10141 attcttgaaa attataaaca cttgaaaaaa ttaggggtgc ggagtatcat tcaccgtttt
    10201 gagatcttat aactttcgtt tcaggagaaa aatatgcagc acgaaatgag atggcggctt
    10261 gatgaccttt tccccggttt tgactcaaag atgttctctg ccagctgttc cgatatagat
    10321 aaagctatcg aggattacga caactgggct gacggactta agaaacggaa agaccctgtt
    10381 gaaaagataa ttgaagaata tatcgcaaaa agaaattccg caggccttgc actttcaaga
    10441 ctttcagatt ttgcggaact cagcttttca gtcaatactg ccgatcagga agcggttaaa
    10501 ttcatggagc tttttgaaaa gaagctcagt cagatcgaag tgatagatgt gaaattcgtc
    10561 aaatggatcg ttaagcataa gcatgcagat cttctaaacg gaagtgagtc cctgaaaaac
    10621 cattcgtttc acctttcaga gatgataaga aaggggaaat acatgctcga cagttcaaag
    10681 gaaaagatca tttccgatat gaaggcaact ggatcttccg catggacaaa cctacacaat
    10741 gtccttacca gttcacttct tgtcgatttc actgaaaacg ggaaaattgc tgaacagaaa
    10801 acacttcctc aagtcagaag catggcatat tctccagatc cactgacaag aaaaaaagct
    10861 tacgaagccg aacttgcatc atatcctaag atcgaaaaat cgatatgtgc atgcattaac
    10921 ggaataaaag gggaagtcat tacgacatcc cgtttaagag ggtataaatc tcctcttgaa
    10981 atgacccttt taagaagccg gatggacaaa agaacacttg atgcaatgat gagatcactc
    11041 aaaaaatatc tcccggtttt cagaaaatat ttcaggaaaa aagcgctgat gctccggcac
    11101 aggaacggtc ttcctttttt tgatatattt gcgccgatca gcaataccga catgcagttc
    11161 tcatattcac aggcgaagga ttttatcgtt gaaaaattct catctttcag tgctgatctc
    11221 ggactttttg ccaaaaatgc gttcgagaac ggctggatcg atgctcaacc gagagaagga
    11281 aaagtcggag gagcattctg tgcgaatttg aggagcataa accaaagcag gatactctca
    11341 aatttcagcg gttcatataa cgatgtgaga actcttgccc atgaacttgg acacgctttt
    11401 cacggacact gcctcaacaa tgaaagtttt ctcaactcaa attatccgat gccgcttgcc
    11461 gaaactgctt caatattctg cgaaaccatc gtgaatcatg ccgcactgaa agagagcaca
    11521 cctgatcagc agctttcgat acttgaagcg gaaatatcgt ccgccggaca ggttatagtt
    11581 gatatttaca gcagatacct ttttgaaact gaagtgtttg agaacagagc ttcttcatca
    11641 ctttctgtcg atcagctcaa ggagatcatg ctttcggcac agaaagaggc ctatgccgaa
    11701 ggacttgacc ctgaatatct ccacccgtac atgtggcttg taaaacctca ttactatgtt
    11761 gcggactaca atttctataa ctttccttat gctttcggac tcctttttgc caaaggactt
    11821 tatgcaatat atcttgaaag gggttcttca tttgttgaag attataagcg gcttctttca
    11881 gaaaccggca gaaaaagcat actggatgtg ggaaaaagtg tcggcattga ccttaacaac
    11941 acatccttct ggaacggttc ttttaaagtt atcaaggaag atatcggcag atttctgaaa
    12001 tattagatca gttcacactg ttagcaggtt tttttccttc aagcactgac agacatccgt
    12061 ttatggtcat ttcagccatt tcagctctgg tctcttcagc ggcacttccg atatgcggca
    12121 gaaggacagc attattcaac gcaaaaagct tctgatttat gaccggttca tattcataaa
    12181 catcaaagcc cgcggcatag atcctttttt cgatcagagc atcagcgagg gcatcttcat
    12241 cgaccagagc gcctcttgca gtgtttatca gtattgcatc agatctcatt gaagcaatct
    12301 cttttttccc tatcagatgg tgaagctccg gaacgaaggg aagatgaaga gaaatgaagt
    12361 gactgttcct gatcagatca tcaatctcac agtattctgc ggacagctcc gtctctttgt
    12421 caatgcttat ccttgttctg ttccagtaaa cgattttaag tccgaatgct tttgcccttt
    12481 tcgctgttgc cctacctatc ctgcccatac cgataacacc gaaagttttc ccctttaaag
    12541 atttcccgag aaaaaggtcc ggctcccagc cagtgaattt tccttctctt gtaaattttt
    12601 ctccttcata actccttctt gaagccgtaa gaagaagcag gatcgcaaca tctgccgttg
    12661 aatcagtaag aacatcaggg gtgtttgtca cagttatccc tttttcactg cagtatttca
    12721 catcaatgtt gttgaatccg actgcgtaat tggaaataac ctttagacgg ggtgccgcag
    12781 taatcactga cttgtcgatc ttatctgaca gaagagttat caacccttca ggatcgaacg
    12841 atgaaactgc attgcgaaga tcttttcttt cttccggact aagaactttg acctcgacac
    12901 cgctttcatt taaaagcttt ttgtatcttt cgccgggcag ttctgatgtt aaaattatcc
    12961 tttttttcaa attatcctct caataatatt gttgttcgaa tttcaaaccg aattcaagct
    13021 gtcttgcata gaagctttta actatctgaa aattcctgtc atatttctgg ggaagaaaat
    13081 ttctttcaat gtggtggaac aggaatgggg aaacaggcgt gttctcattt tttcttagaa
    13141 aatatacgac tccgcctgaa ccgtcctgaa gaacaaatcc gcttttccct atcactgcat
    13201 cttttttgta aagagagttc tttttcaatt tgtcgttaat gtgtcccagc cccttgaaaa
    13261 aagcccgggt cgcatagtta ctgtaaataa attccatttc tccgcctgag tttcttgaat
    13321 gatcgacatt cacgagcttt gcaagaaaag gaagttttac attatgatgc attctcacat
    13381 taaaaaccgg aacaagagta ttgctttcat tgataaatct gacatcagga cagccgtcaa
    13441 aaggtccgtt tacaatacag ggctggagat aactgccgtc tgaattcaca tatctttcac
    13501 catcgatcgt ttcaaaaaga taaacatctg aaagggtttt tcttacagca tttttaaacc
    13561 tgaggtaaac tattttttcg ttctgttcgt tgtaaaacag tgttactctg tctctttcgt
    13621 tgaaccagat cttataatcg tttagaaaat acacgatctc ctgaatattc ctgataagtt
    13681 tgtcagacag tcttttgtca tactttctgc gatacgaatc tatcatggaa cctttaaggt
    13741 agaaatttgc aaaaattatg ttgtcgccct caagagaaaa attcatatat tgaggatttt
    13801 ccggatcgta actcagctca aaatctattg atgagtggtc ggcaggatat atgaacactt
    13861 ctgtgctgac agaatcctca acacattttt catcttttgt aaaataatag atgttcatga
    13921 ttacggaacc cgcaacgata aaaataatga aaagtttagt cttcattttt aaaatccggg
    13981 aatttcagtc tttccggtat gaaaagtttt gtgtaaagat ggatcgcata ggaatcggta
    14041 agacttgcaa taaaatcagt tatgttcgtt tcgggatcat cacttacagg attagaatag
    14101 aattctgtaa aattgtcata attttcctta aaataataaa aaagtgactt gatgatcttt
    14161 tcagattttt caaattcgcg tcttatttca ggcatttcat aaatgttgga gaacataaag
    14221 tctctcagat cctcgatcat cttcgtggtt tcactgctga acctgattat tttctctttt
    14281 gcttcgatag cttcgaaact ttttgaaatg aggtccgtca ccatgtaatt tatccgctga
    14341 gacccttttt cacccagaat gtcattgtgc ggaacctttt ttatgtcaag gaagccgcct
    14401 ctgacagcat catcgatgtc atggttaaga tacgcaatta tatctgcgag tcttattatc
    14461 tgaccttcaa gagtttcagg gagagcatct tttttcaaaa gaggtccctt tcctttcgaa
    14521 tgcatcagga tcccgttcct cacttcatat gtcaggttca gcccctgacc gttccgtgca
    14581 aggaaatcga caacccttat gctctgcttt tcgtgtctga aaccgttctt cagcagtttc
    14641 ccgagagctc tttcgcctga atgtccgaac ggggtatgcc ccaggtcgtg tccaagccct
    14701 atcgcttcgg taagcgactc gttaagaaaa agtgctttgg cgatcgttct tcctatctgg
    14761 gtcacttcaa gagtatgtgt aagccggttg ctgtaatgac ttcctttcgg tgaaaggaag
    14821 acctgtgtct tgtcaccgag ccgtcgaaat gccttggaat ggacaattct atctctgtca
    14881 tgctggaatg caggtctgat gctgcacagt tcttcatatt tcactcttcc ttttgaagca
    14941 gagctaagac aagcggccca cgagagctct cttttttcga tctcttcaat gtgctttctt
    15001 atattatcca tcgcggtctt ttcccttata aaatgtcaga attaaaacaa ttacagaaac
    15061 tccgaagaga gcgtatatga caaaaagata aaattcgttg tcaacagacc cggccgccat
    15121 cattgatccg gtaattatga aagccgagca gatccttgtc accatccttg aaatgaaact
    15181 cctttccgat cttccgaaaa ggacaaggaa gcggtgctgg atcgttccat cctcgatctt
    15241 ttccgcaatg ttggaaagaa ctcccggaag gttcttaagc agatctatca tttttataag
    15301 aagcgaaaaa acatctcctg ctatcctgtc gggtgaatat ctttcagcaa aaagttcctt
    15361 tgaaagtttt tcagcgactg tcatcagttc aaattcagga tcaagttccc tgcaaagccc
    15421 ttctatcagg gaaaaactct ttataagctg cgtgagttcc cgcggtatct tgagcttgtg
    15481 tttgaacatg atattaaaag actttttcac aatttcggtc gtatttatct cagacaggtt
    15541 gtcaggcagt gaatcaaaga ctttcctgat gtcacttatg aactccttct tgttaacagg
    15601 ggttccgctt tcgctcatat caatgtaaat atttgcaact ttctcaaaat ctcttgcaat
    15661 gaaagcaatg aacatgtctg cgatcttttc ccttaaatat ctgtctatcc tgcaacacat
    15721 tccgcagtct attgcgacaa tatgattccc cggcttgaag aaaatattgc cggaatggag
    15781 atcgccgtga aaaaagccgt caagaagaac cattttcatc agcgcttttg cacctctgtc
    15841 ggcaattatt ttccggtcga acatcgcctg atctgtgcat tcaataacat cgtcaagctt
    15901 aacgccatct attctctcca tgacaagaat tctgccggtg cagtattttt cattcatttt
    15961 cgggaaagag agccattccc actgttcacc gtcaaaattc gatgccagtt tctgggtgtt
    16021 tgatttttca atgacaagat cgagttcctg attcattgtc cagaaaaact cttcggctat
    16081 ccctttgaaa ttgatgttct ttgaatacga tgatgtcttt tgaagaatat ctgcgatcca
    16141 gattatgatc tccatgtcgc tcttgatggt ctcgaccaca ccgggctttc tcacttttat
    16201 tacaacactt tcccctgttt ccttcaggat tgccgaataa acctgtgaaa ttgaagccgc
    16261 cgcgataggg gtttcattga aattgtcaaa gatctcctca agcgatgcag caagttcttc
    16321 ttcaatgatc ggttttataa gtgcaaaatc catcggattg acattgtcag tgagcttttc
    16381 gaactcttcc gccatcgaaa tggggattat gtcgccccga agagacagga tctgccccat
    16441 ttttatgaaa gtcgccccca gttcctcaaa tgcaagacgg aacctttctt cgagcttcat
    16501 atccttgaaa gatttctcct cggcatttct gttctcaagc ttaaaaacgc tcagaagggt
    16561 tctcggaatg aacggggtaa gaccgtattt ggccgcaaca gcacctattt cgctgaatct
    16621 tttcaatctt gaaaatgcag ccatttcttc atcctccctt atcagtcaac ggttatagat
    16681 ttgctgacat ttttcggaac atcgggatgt actccgtgat tttctatctg acttctgttg
    16741 agatatttca tcttcgcaac actcatttta aatgcaagaa gctgtaatac gaccgccgct
    16801 tcgaagatga atatgttcct gtcgcctgtc cttggaagtt cgatgaattt cgaccagtat
    16861 tttcctgtcg aagcgggctt accggttgcg gctttcttga gatcgtcatc gtcttccgaa
    16921 atgagaacta tgtcggctcc cctgatttta tgagtatgga tctgggatat tgttatcctt
    16981 ttgtccctct catcgggagg acacacaaaa acaagaggat agttcgaaaa aaagttttcg
    17041 atacctgtct tttcggcaaa aagactgtac atcttcttca ttgcgttacg atcttccttt
    17101 gatgtaaagg gctttattac agtgtcatcg tcaaccttac ggaatttaag gttcttaact
    17161 gtgttcgtat actctctcac catatcggct tttgcaccgt tcccttttaa aaaaccttta
    17221 tcaaaaagct gttcgaacat ttcgatcata tttccaagaa gaagttccat gtcggtgagc
    17281 gaatatatcg tgttctttcc taaaatggtg ttaggtccgt gcttgaattc agcggcgtca
    17341 taaccttcag tgtggttcag aacgacttct ctgatcttga gagcaccttc ttttgcaagt
    17401 cctgtcagcg atgtgccgag aatgtgtatc gaaggtttca tgaaaagttt gagcgctgtg
    17461 tcggatacag gaatgtcgat gttcttaagt gtgaaatcca tgaaatatct tatctttttg
    17521 agatcattga aaacatcatc ctcttcgttt ttcacaagtg ccgcaaggat atagaaaaga
    17581 gcgatctggc ttatgaaaga cttggtcgcg gcaacagcga tctctgaacc gcacatgatc
    17641 ggaagataga aatcactttt ttcctgcgga agtgtcgagt tttcgttgtt cacaattgta
    17701 agaagctgaa ctttgtcgct gcaaaccttt ctgatgtcgt tgaaaatatc aacaaggtct
    17761 ttcgtttctc cgctctggct tacacccacc acaatatcat tttccctgag cgaatttatg
    17821 aaacccgacc tgaaaccgcc cggatttgca ggtattattg atatgtctgt cagattgttg
    17881 aaaaaatatg caccggtaag agcggcatga tatgatgttc cgcttgctac gaaatatatt
    17941 cttcctcctc tttttccgct ttctttaaat gcattgacca gtttatcacg gtattttatg
    18001 atcatcctct tctttttcca cacaagcatc gaatcgatca gccagagctc ggtaaaatat
    18061 ttttcattta tccccagaag ttcttcaaga agggtggtct cctcagattt aaagacaaaa
    18121 tgcttcggat ctgcacagta ttttgatgct tttgcaaaga tcgttttaaa agttttcagt
    18181 ttgcggatgt tttcaaattc ctttttaagt tcattgctgt cgtaaacatc atacagagcc
    18241 aaaagggaat acaatatctc tcttgctgta tctttgtttt tttcaaatat tgcatgaagc
    18301 tccttctctg actcatcatt gaagtagtaa agcatcagat tgtcgatatt tctcggtgaa
    18361 gagaatatct cctgctccat gaaaaagtgg aatttcggct ggagctggat gtctgcgata
    18421 ttaagccggc ttctgctgag tttggtcacc cattctttgt cttccgtgag tgaaaaaagc
    18481 ttgtagctgt cgttatcaaa atagatccct tccccttctg acatcgggat aaggaacctt
    18541 gtctttgaaa gtatactggt aagatcactt gagacgacaa tgaagttccc ggcattgtca
    18601 gaaccctttc ctgcataaag tgatgatccc gcttttatgg cgaaaactcc cggaatttca
    18661 ggcagagtga cacatgcggc ataacttcct tcgatcattt tctgtgcttt tctgatcgct
    18721 cttatcatca gcgcgatctt ttcatcattt gttgatggct tgcatttatg catgagagct
    18781 gaataaaagt gctcaacaag atggaccacc atttcaccgt cattgtcgga aagcactgtg
    18841 tgtccgttgt cggcaagaaa atccttgagc ttatcagtgt tacttacatt tccattgtgt
    18901 gccccgacca tgtggacaag gcagtttact tcgtgcggct gtgcattgac atcagttacc
    18961 gctccgtaag tcgcccatct aacctgacct atgaacttga acccgctctg tttagggatc
    19021 tcaagctgtt caataaccct tgagggggct ccgactttct ttctcaaagt gacagtccca
    19081 tcctctttta tgaaagctcc gccggtcgag tcgtatccgc gatactcaag ctttttaagc
    19141 agtgatgccg cttcatttcc aagatttgca atgttttttc tatagacgat cgaaacaact
    19201 ccgcacataa atttctccgt taattcaata tgttaaatcg aagcagtcat tttcttaagc
    19261 cactctttct cttcttttcc aagttgcggg ctcaggtttc tgaacacatc tttatggtag
    19321 tcgttaagcc atttcttctc ttcagcatta agcattccgg gttcaattgc cctgatgtcg
    19381 ataggacaca ttgtaatatt ttcaaaaccg aggaatttgc cccattttgt ctcttcgatc
    19441 tcttttacaa ggacaaggtt ttcaattctt atgccgtatt ctcccgcttc ataatatccg
    19501 ggctcgtttg aaagtatcat gccgggaacg agctttgttt caagccatct gggtgatatt
    19561 cccatcggtc cctcatgaac gcttaaatat gcaccgacac cgtgaccggt cccgtgatta
    19621 tagttctgcc cgttcttcca aagagcaatg cgggcaagtg catcaaggtg tgttcctgct
    19681 gtaaaaccct gcggaaattt cgctgttgaa acattgatgt gaccgatcag gacatttgtg
    19741 aaatcctttt tcatcttttt tgtcagtttc ccgacgacaa ccgttcttgt gatatcagtc
    19801 gttccggtaa gatactgtcc gccgctgtcg accagataca gaccttcagg ttttacttct
    19861 atcgagcttt cttttgtagc gctgtaatga attatcgctc cgtgttcgcc gtagccggaa
    19921 attgtgtcaa agcttaagcc cgcaaaattc tcacctgccg ctctaaatgt ttcaagtttg
    19981 tctgaagccg atatttcagt aatcttttct ctgccgagag ttttatcaag ccagcagagg
    20041 aacttcacca ttgcaacgcc gtcggtgatg tgggcttttt ttatcccttc gatctccgta
    20101 gcatttttga cagctttcat aatctgggac atgttcatcc cttttacaac ctcgaccttt
    20161 tttgtgtcaa ctgcatcaag aagggactgc gaacatcttg ccggatcaag gaaaagcttc
    20221 cctttgccga gcttttcaag aactttgtat attttgtcgt aatctcttaa tttcacgccg
    20281 tctttttcaa gagttttaac atcttttgcg ttgaatttgc cgtttttgac aaaaagctct
    20341 gcacttttct ttccaatgag tccgaaaccc atgacgaccg gattacagct tacatcgttt
    20401 cctcggatgt tgaagatcca tgcgatgtcg tcaagagatg caaaaaggtg atagtcaact
    20461 cctgactgag ccattttctc acggatcatt ttgaacttat ccggtctgcc tgtccctgca
    20521 tatttgatcg ggtggaggac tgctctggct gaagggaatg ccggtctatc cttccagata
    20581 gcgtcaacaa ggtctttccc cggtttcact ttcagccctt ttgattcaaa acttctttta
    20641 agatcgtcaa cttcatttat tgacatgact gatgaatcaa aaccgatatt tgcccctttt
    20701 ttgagttctt ttgtgagcca ttccttgtaa tcgggagttt caggaagacc tgctttaaaa
    20761 agcgtaatcg tgctgttttt aagctgctct tccgcctgaa gaaaatatct gccgtcagtc
    20821 cagagccctg cttttttcat cgttacgacg actgttcccg ctgaaccgtt aaaaccgctt
    20881 atccattcac gggttttcca tctcggggcg acatattcac tctgatgcgg atcagcactc
    20941 ggaatgatat atgcatcaat tccgaccttt ttcatcatca ttctcaactg ctcaagccgt
    21001 tttacgatag tcatagaaac ctccaatttt tcatcgttac caaaaaattc agtagtataa
    21061 taaatgagga attttaaata tcaaggaaat ggggatctga tgcttatgga ttctttataa
    21121 atccaaatat gcttgctttc ttttttaccg gttccgatca atcttcttac cgcatcaaaa
    21181 atgatcttga attgtctgtc atattttgtt tccagttcat ctaaaatttg tatgcaccga
    21241 ggttgtcaca atttgtgata agctcagtgt ccatttatgt cgttaaaatt tacacgaccc
    21301 ttatttcgat ctttcgacca aggaccttgg catatctggc aagtgttgag attgttacat
    21361 ttttaatttg tttctcaagt cgcgacatcg cactttctga tgtctccata agttctgcaa
    21421 cttctttgag agtaagccct tttttctttc tgagttctct taaattatcc atgatgatac
    21481 caagtttcat ttcctcatac atttcattca aaatctgttc ttttgcactg tttttatcaa
    21541 caaatctttc aattgtcttg tcaagacttt ctgttttgta acctttattt ctcatgactt
    21601 acctccctga actgttacaa acaatttctt atcatataaa gcaagttttg tcaagtgcat
    21661 ccaaaattct acgctccatc agatttagcc aacaatagat aatgcagcaa ttagattaat
    21721 tttgcaagaa aatggataag attttcttac tcgccagaaa ataaagaaaa gtggagttta
    21781 tttagggtgt tgggatggat ttcagcagaa gaacatatgt
//
LOCUS       DAOOYB010000011        21284 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_2319_length_21284_cov_11.777616, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000011 DAOOYB010000000
VERSION     DAOOYB010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 21284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21284
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2319_length_21284_cov_11.777616"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="PL056_01355"
     CDS             <1..337
                     /locus_tag="PL056_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45692.1"
                     /translation="ECVCDSFYQDEDNNGTCLETCILFDCNDTAWGASNDCSIINGAP
                     VCDCDYFAGYTGTHCTECRSGYHAGGFGYEDECFEDNCEEAEDTFHGWLDCWSFEECR
                     DTFGYAECW"
     gene            complement(345..1361)
                     /gene="rlmN"
                     /locus_tag="PL056_01360"
     CDS             complement(345..1361)
                     /gene="rlmN"
                     /locus_tag="PL056_01360"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HPM45693.1"
                     /translation="MEILEFTRQTFENAIESAGYPKFRARQIIRWIYHRDVYSFEEMT
                     DLGKDDRVAFQKLFSITPISEPAIHSSDDGTKKLTFTLEDGAVIESVIIPEDKRNTLC
                     ISTQAGCPLKCSFCRTGTLGFKRNLSVREIVGQVIRADNILEKTGEHLNNIVYMGMGE
                     PLLNFENVVDSILILMDDLMLNFSNRRITVSTAGIADRITELGEKTAVNLAISLHAVN
                     DEKRNKIMDINRKYPIAALINAARYYPAHQRKKVMLEYIMLKGFNDTAEDAKELAKIA
                     NQIDAKVNLIAFNTFPGAIFEPTPMKEILRFQEILISKNVTALLRKSRGSDELAACGQ
                     LGHV"
     gene            complement(1363..1980)
                     /locus_tag="PL056_01365"
     CDS             complement(1363..1980)
                     /locus_tag="PL056_01365"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPM45694.1"
                     /translation="MEIKVLQVAPIGTNCYIVSENNEAFIVDPGGDSADIIKEAQGKS
                     VKYILLTHTHYDHIGALNDIKKQFPDALLAVHSLEKEWLYDPKKNLSIGFYIDFAYSG
                     KVDIELKDGDELEFAGHSIKVIHTPGHTPGCVCYHIEDDLFSGDTLFKSSVGRTDLPG
                     GDGISLVKNIKEKLYVLPGKTVIYPGHEGTSTIAWERTRNSFVKL"
     gene            complement(1995..3497)
                     /gene="malQ"
                     /locus_tag="PL056_01370"
     CDS             complement(1995..3497)
                     /gene="malQ"
                     /locus_tag="PL056_01370"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HPM45695.1"
                     /translation="MPLNKKTNNRKAGIILHPTSLPGKWGVGDMGGNALKFIDMMADA
                     GIRIWQILPLTPTGIDGCPYNSYSAFAGNELLIDIEDLLKSGLVPEDVEIPQFNDHRV
                     DFRKVIDFKNKLIASAAREFAGIKKESGEFEIFKKDHAQWLDDYALFKTLTDKFEGRS
                     WKLWPEEYRKRDVSALKKFASEHKTEINRYQFGQWIFFSQWKKLKSYANSKGIEIMGD
                     IPIFLSFDSADVWANQHIFKMVEGELLEVSGVPPDYFSENGQLWGNPLYDWDKLKKDD
                     FRWWIERFSHNVKMYDSIRLDHFRGFSEYWSVPSSENTAKNGVWKKTPGKELFNAVIK
                     ELGHFPVIAEDLGNIDEKVIKLRDEFGFAGMKVLHFAFYDGTDHEFLPHNFKDTNCAI
                     YTGTHDNDTTAGWYWSQSSDTRNYVNHYLGSNGDDIHWKMIRLALSSIADLAFFPLQD
                     VLGWGSDCRFNVPGTTGPQNWSWRFKEGDLTNNHIDKLRGVLVAFNRVHK"
     gene            complement(3510..4016)
                     /locus_tag="PL056_01375"
     CDS             complement(3510..4016)
                     /locus_tag="PL056_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase"
                     /protein_id="HPM45696.1"
                     /translation="MKRISAEIFVLKDGSGNLINDIDTDQIFHNKYLSIVDIKLMGQY
                     ALDNLDGYKDFAKKVKSGMVLVAGENFGSGSSRQQAVDCFISLGMQGVAAKSFGSIYK
                     RNIINTGFPMIEITDIDEKLLETGTKLEFDIETSEIFNNGKIAGHFKKFSTVQKDIIE
                     KNGLLNCL"
     gene            complement(4016..5968)
                     /locus_tag="PL056_01380"
     CDS             complement(4016..5968)
                     /locus_tag="PL056_01380"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HPM45697.1"
                     /translation="MKKVKVCITILFALTAFFMFSCTGKNEVDKKTEKESAEARQDDE
                     IFKVEIGTSMFQGPADAPVTIINFSDFQCPFSKRSVDLMSKIMKDYDGKVRYVFKHFP
                     LGFHKLAKPAALASIAAQKQGKFWEYYLRLFDDIKNINDENLVKWASDLGLDMEKFEQ
                     DRNSEEAGKILQNDISQGMTFGVRGTPTMFVNGRRVVGANNVKITELLVKQISEGEKI
                     KAKGVKDVYSEIVKDGQIRYTPPKRDPGNVPSDVYKVGIPEHSPVWGVDDADVTIILF
                     DDFECPFCSRLHTTYEQIKKEYEGKIRVVFVNLPLRFHKKATPAALAALAAHKQEKFW
                     DMYDSLFKKQKEWKDAPDLQKWFEDEAKLLELDIEKFKKDMESPQLKKMVEDDSALAE
                     KLGVRGTPASFINGRFVNGALPFETFKQVIDEELKKVQPLREKGLKGNDLYNEMIKDG
                     KPGISRSAGKEKPASDPDKIYELSFTGKEPVKGKKDAGITIVEFSEFQCPFCRRGAST
                     IEEVVDEYKGEVRLVFKHMPLAFHKEAKPAAKFSIAVKEIYGNEKFFALGKMLFDKQS
                     EWKSDHMKKFESYSKELGLDWKKISKMMESPETDLILKQDMEEGSKHGIRGVPAFFIN
                     GKMVSGAKKKEYFKAVIDNLLKKEKK"
     gene            complement(5980..6468)
                     /locus_tag="PL056_01385"
     CDS             complement(5980..6468)
                     /locus_tag="PL056_01385"
                     /inference="COORDINATES: protein motif:HMM:NF016489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HPM45698.1"
                     /translation="MRLIYYAWATWFGSGYSPVAPGTAGSLAALPFVWFFAGLGFFPL
                     LIFAVVTFITGVAVSQYISTDSSADDPQIVVIDEVCGMAISMLLVPSEYLNLFSLNSW
                     VVVLSSFGLFRLFDVWKPWPASYFDGSVHNGWGMMLDDVAAGIYSMIGTFLVYKLFLS
                     VL"
     gene            complement(6469..8529)
                     /locus_tag="PL056_01390"
     CDS             complement(6469..8529)
                     /locus_tag="PL056_01390"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HPM45699.1"
                     /translation="MTAFTSDALRNICVAGHGTSGKTTLVESLMFNAGAVNRQGTVED
                     GNTVSDFTEEEKNRGISISASVNGCEYKKCLLSFVDTPGYFDFAGEVAATIPFCEAAV
                     LTVKGSSGLDAGTQNAFNTAKKLHKSIAFFVTEIDRDNINLDDAFDSISKELEIPLAP
                     ITFPAGSGMSVDSIIDVFRGKLIKYANGKRASESEIPAEFADKAAQLKEKLMEAVAES
                     EESLMEKYFSEGTLSDAEITAGLAKAFANGSVFPVFFGAANKGVGVDCLLDAVVDFFP
                     SPEGKVLKFAEGEIEMNPKTSPKGIVFKVNTIPQFGEVFIFKLLQGEISEGADIYNIN
                     SSSYEKFGQFFYTRGKERVSIKTVTAGQIAATVKLKNTKTGDQFADNKNAEIFPFKLI
                     EWPEAIVRGAFMGVSKEDTDKVAAAIKKMQVEDASFKFIQDAELKQLFVDGLGELHLE
                     VISKKIKNQFGLDVKIVEPRVPYRETIKGRAEARYRHKKQSGGSGEFAEVNIVLEPTP
                     EAEFEFVDAIVGGVISNRFIPAVEKGIREILPEGVLSGCKFINCKVTLNDGKEHPVDS
                     KEVAFKKAAQMAFRDAAKRANPIILEPIYNMLVKVPEEFAGSVMGDISSRRGKPQGME
                     SEGKYTIVRALVPLKEIHGYSTQLRSMTNGRGTYTISFDHYEPVPGDVQKKIVEEYEA
                     SKEQ"
     gene            complement(8545..9345)
                     /locus_tag="PL056_01395"
     CDS             complement(8545..9345)
                     /locus_tag="PL056_01395"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HPM45700.1"
                     /translation="MNISNSRLTEIAGTVKEAGDFAKTMFNVPGLRKKFKSDRDLVTE
                     ADELVEKMLLEKLKRIDDIPFLSEEFNPATTLNDPCWIIDPIDGTTNFISGIAPFAVS
                     VAHFNGRHVDAGVCFLPVTDELFYAASGGGAFLNGKKIKVSDTDKAINCVAATGFADI
                     THESDQSTLAVFNSIIKKTRALRRLGSAVADLVYTACGRFSFFYEAGLSPWDVAAGSL
                     IVKEAGGIVTDFYGNDRYITGMTIIASNRQMYDFVKNEIDELYIKKTS"
     gene            complement(9329..9610)
                     /locus_tag="PL056_01400"
     CDS             complement(9329..9610)
                     /locus_tag="PL056_01400"
                     /inference="COORDINATES: protein motif:HMM:NF020473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HPM45701.1"
                     /translation="MEKKCKKSDLSSVVNPSFESLVSSIYSAVLFNLGEIKVKGIDSV
                     CENVDMARFNVGLLEMIEEKTKGNLSEEEKTYLKNVLLNSRERMSEYLE"
     gene            complement(9629..9802)
                     /locus_tag="PL056_01405"
     CDS             complement(9629..9802)
                     /locus_tag="PL056_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45702.1"
                     /translation="MDFSKFDVRVVERKLKSGEITEKDYKKYLESLEDANEHVEINEE
                     QLLKDAGVRKKEH"
     gene            complement(9826..11322)
                     /locus_tag="PL056_01410"
     CDS             complement(9826..11322)
                     /locus_tag="PL056_01410"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HPM45703.1"
                     /translation="MRSLVTYLLLCSFSFLSLTPSGLTLAQLRADDDFELDELDLGLE
                     DEEDVKEPEEEVEKPGKDSKKSDYSFDDAEDSAESDMEDSEGSFSEDSGKQKIAAFYL
                     FPDSFSQKSSGNIVAETASYLAGSEYDYLKTEAALFVPSMSVAKRDLEEGLKTFEEAK
                     ALYNDLSIEEAITKFQSARKTIEKHLDKIPDMKVLGDILLYLGASYKMLDEDDEATPL
                     FSSYLSIYPDAELDDIVFSPEIISFFDRIKEDFLMLPNGSVRVESFPEGALVSINGKI
                     VGITPTKVHGITEGRHYYRIHKNGYRDRAGTFTVRERKESKIAEDLSKYDNAGFLHEA
                     ESFMVKEYGRLAMLRKCVEIAEKMKVDSVLVTSLTVDGETVKYSGHIVNREKRNFKKV
                     ETSFLLPDRKDLTEVDELQKFNKDLINDTYGFKPISDVALDEAELLGLDTKKDKKDKG
                     PKTPIHKQWWLWTLLGVVVLGGAGVGTYFAVTASQGKESGASLEINFK"
     gene            11486..12460
                     /locus_tag="PL056_01415"
     CDS             11486..12460
                     /locus_tag="PL056_01415"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPM45704.1"
                     /translation="MQFQTDFDICFSVLENSGIRADLFLSSLLNISRSRIQKNIALEN
                     LSVNGKVIKKNSYSRFQKGMVVEFEIEPVEQICAEPEKMDLDIVFENDLFLIVNKPAG
                     LVVHPGTGNYCSTLVNGIIHHLNWSGDRLKEGDPVRPGLVHRIDKNTSGLLVIAKTQA
                     SFEELSLKMSVHDITREYVCIVRGHLKQNKGTIDTFHGRDPSNRLRFSPNVKNGKRAV
                     THYEITAEYKYCSLLKVTLETGRTHQIRMHMSHIGHPVANDLLYGGLVKTDDPVLNKK
                     LNSCPRQLLHAKTLGFDLCGQNYLFEHEIADDMKGIITYLESISGGEK"
     gene            12457..13626
                     /locus_tag="PL056_01420"
     CDS             12457..13626
                     /locus_tag="PL056_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45705.1"
                     /translation="MKEKSLLLFETKELTAQKYFDFLKDTPWEIYHKQNLETFLETLQ
                     NDTFDLIIVEESMVPEEVVALLAESDTPVILSTNSSKTGRLTTILRDFSSADLINAIG
                     KSSFLKIVRQKDGVSYEEPEHDDEDEPVLLEPVFEENSLDAVVLTPEKGDGKTKSSWE
                     VPEKKGTESEPVLEKKPEEKKDIFARIDEIDSILSSLSRDIETQKPEPKTSAKPGFQF
                     GDADDDAQQEKTLFAVQPDVKEEVSRTAENKNENADKNEDKNADFLFDDDYKYDAKAV
                     EPDKKPAVQRPEFDNNMVNDFEAILSDKPLNVPNVKAEEFEIETHDEKIREPEPSEAE
                     SSSNVEKKAAQTEDKTLLNEDITKEEIRNWLEKNGRQIIKEIVLEQLSKLSGKDG"
     gene            13619..14086
                     /gene="ruvX"
                     /locus_tag="PL056_01425"
     CDS             13619..14086
                     /gene="ruvX"
                     /locus_tag="PL056_01425"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HPM45706.1"
                     /translation="MVKKIVSLDVGIKYVGVASTDPLWNFPVILGTLQRKQSIADDLA
                     RLSVMLKDIDVCGVVIGFPLTMEGHEGSFAPVVKGFERRLKELFPGIPFFRIDESLSS
                     KEAVEIKQIKPGNYRKNKKQGKIDSTAAAIILERFMGTHEFENLKFSVLRTGE"
     gene            14089..14319
                     /locus_tag="PL056_01430"
     CDS             14089..14319
                     /locus_tag="PL056_01430"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HPM45707.1"
                     /translation="MKTGILKISGIFCGGCTYAIEKAGRRLEGVSEVRVNPGTGEITV
                     NYDGGPEVLEKIVRIIETLGHESEIAELDRSH"
     gene            complement(14316..15908)
                     /locus_tag="PL056_01435"
     CDS             complement(14316..15908)
                     /locus_tag="PL056_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007062036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HPM45708.1"
                     /translation="MISTNKLSLAFGGSKLFDDVNVKFTPGNCYGVIGANGSGKSTFL
                     KILEGAIEPTSGEVVVQKGCRIAVLSQDHFAFDDFEVLETVIRGHELLWKTMLEKDAI
                     YEKEDFSEADGLLASELEEKFSALGGWEAESDAATILAGLGITEDYHKKKMKVLDGGE
                     KVRVLLARALFGKPGILLLDEPTNYLDIRSIMWLENFLINFENTVIVVSHDRHFLNKV
                     CTHIADIDFKKIKMYVGNYDFWYESNQLMQKMIKEQNRKIEDKRAELKQFIERFSSNA
                     SKAKQATSRKKELEKLTLEDMQVSSRRFPYVDFKPAREIGKELLTVENLSKTIDGVTY
                     FKNISFRLEKNDKVALVGSDDMAKTVLFKILAGELKPDEGDYRWGPTVSFAYMPKDIS
                     SYFLNSDLDLIDWLRQYSEEKSENFIRSFLGRMLFKGDDALKSCSVLSGGEKMRCVLS
                     KMMLSTSNVLILDEPTNHLDLESITAVNKGLVNFTGTILFVSQDHQFIETLANRVMVF
                     NEEGFMDAKIPFDMYIEKGGRV"
     gene            complement(15936..17348)
                     /locus_tag="PL056_01440"
     CDS             complement(15936..17348)
                     /locus_tag="PL056_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45709.1"
                     /translation="MFKILLLIAVFVSVPSVVEARSCADVDASFKKMITEMKEVWKKD
                     DAQLEKEITELEKTITHLEKNVDADMRKLDKMINSEKEKELEKAKVQYEEEIKSIDAA
                     EKTFVSKFEKNIADGTAKIAKMTKEIEDIDKKNKDLDKVKEEIDANYLKEQHAKGVDI
                     DKIRMANEDEDRKYLAEISAEYQVKFDGFNAKLEVWEKKEVEKIAVVEAVDKEIEDFS
                     VAMANKLGDFQSDIEKRREELAKKFKDEELEAKSMELDMELAKRSEEMNRELGEYRKK
                     KLEEKKQREFELDKFRMEKHDFKKQNDAQIKSAAKIRDNKIELRRNQRKRAEETWDRE
                     KKSMETRYKTTLDLKFTKPLEQLLKKRSAIEREIIKVNDKIAEDRGKSRKDRDAFERS
                     IQERRANNEEIYRAQREGLSSRYDGQLLMMKQKEEKKKNDLELKRTQLIEKREKNREK
                     RQALLDKKKEQYEKEKSKCK"
     gene            17461..19380
                     /locus_tag="PL056_01445"
     CDS             17461..19380
                     /locus_tag="PL056_01445"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HPM45710.1"
                     /translation="MLLLSDISKQYAGQPILKHVTIPLSKKRTALVGMNGSGKSTLMK
                     IIAGIEYADNGEITTKRHAVVEYLPQQAVYDFKGTVYEEVSAWLNSDRFNELSAREAE
                     IIRILENDLNPDDSLCEELAEVVEELHTLDVKKKRKNSVETILLNLGFSKEDWDRHAK
                     TLSGGWQMRIALARVLVREPDIVLLDEPTNYLDIATIDFLADWMMKFEGQVLLVSHDR
                     DFLNRVVEETWEVFAGNVSIYRGNYDFYLVEKEQRLRVLEKQREKQLDEIKILNDFYE
                     KNRFNAATAPLAQSKYKQMEKLKAELITLPRTPSKMTFRLPDPKRGGEIVAEVDKLYH
                     SFGDIDVIADYKRIISRRERIALVGRNGFGKSTLMNIIGGEIEPLSGECRLGKDVEVS
                     YFRQHEITLLPPDMSVIQFVESIAPFDLMSRVKTLLGCFLFYEDDWDKRIGVLSGGEK
                     VRLAFIKMIMNPGNLLLLDEPTTHLDIDSKEILLKTLQNIDATIVFVSHDSYFINSLA
                     TSVVYFRGKCDIINFPGTYNEYLDRYGHDIIDEEKAKSGGTDAPLSKGKMDFAQQKEI
                     RNKINRLKKEIENTQKEISDLEKEKNSLSNQLNSSSGNQPEISKKITEIDSKLLKLME
                     QWEEKTEEMERFSAV"
     gene            complement(19393..>21284)
                     /locus_tag="PL056_01450"
     CDS             complement(19393..>21284)
                     /locus_tag="PL056_01450"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HPM45711.1"
                     /translation="VIKDCTKDEDCVYLDGECKKGICSEAGICEIENRPETTLCDDGV
                     FCNGEDACNNNGECVAGNTYPCGDFFCSDSLKSCCEGGFAGDDCSQCVRFVKESGVKT
                     PDGLTWHTAYSTIQEAVDSANDAVLIESNGIDECEIWVAQGTYVIPATIKIYSNISIL
                     GGFAGEGDKRDNDPLLTVLDGSVNKMDNIIDATGASNVVIRSLKFFGNVNNGTGDNYA
                     GALYVSQASNFVINSVIFDSNGAVGASADNGYEGRGGAVAVIESDVTFSDCEFKGNNA
                     QNGERPGGLSTNASAYGGAVFVYLGNVTFSNSTFFSNTVTKRENGTASGGAIFAYAPA
                     SLTISGCTFEGNTTQNWGGALRAEGGNLVLSNNVFTGNSASENGGGVEFNNVESAAFS
                     ECTFSKNTAATGGAIHLSNNSRNITIEGSVFSENEATTLAAGISVSTSQNSIVITQSE
                     FMLNNCKGGTECRGGAIGIDNNASATIVNSLITKNNAKSYGGGLYVRNDSSANLIFST
                     LADNTTDGSSSTTGSGIHNYDSAVTNIYSSIVHGNGPANQTQNNTNSATNVTYSLFQG
                     GYSGTGNINADPLFVDRAANDYHLGAGSECIDKAGTAAENPVIDLDGKTRPDGSGFDM
                     GCYEF"
BASE COUNT         5986 a         4856 c         3963 g         6479 t
ORIGIN      
        1 ggaatgtgta tgtgattcgt tttatcagga tgaagataac aacggaacct gccttgaaac
       61 ctgcatcctc tttgactgca acgatacggc atggggagca tccaatgact gttcaattat
      121 aaacggagcg cctgtctgtg actgcgatta ttttgccgga tataccggaa ctcactgcac
      181 agaatgcaga tctggctatc atgccggcgg attcggctac gaagatgaat gttttgaaga
      241 taactgtgaa gaagccgaag acactttcca cggatggctt gactgctgga gctttgaaga
      301 atgccgtgac actttcggat atgcagaatg ctggtgatta ttccctaaac atgccccaac
      361 tgtccgcagg cggcaagctc gtcgcttccg cgggattttc ttaaaagtgc tgtgacattt
      421 tttgatatga ggatttcctg aaacctgaga atttccttca tgggtgtggg ttcgaagata
      481 gctccgggga atgtgttgaa tgcgataaga ttgacttttg catcgatttg attggcaatt
      541 tttgcaagtt cttttgcatc ttccgcggta tcgttaaacc ctttgagcat gatatattca
      601 agcatcacct tttttctttg atgtgccgga taatatcttg ccgcatttat gagggctgca
      661 atggggtatt tcctgtttat gtccatgatc ttatttcttt tttcgtcatt aacagcatga
      721 agagaaatgg caaggtttac agctgtcttt tcaccgagtt cagtgatcct gtcagcaatt
      781 cccgccgttg aaacagtgat acggcggttt gagaaattca gcatcagatc gtccatgagg
      841 atcagaatcg aatcaacgac attttcaaaa ttaagaagcg gctcccccat tcccatgtaa
      901 acgatgttgt taagatgttc gcctgtcttt tcaaggatat tgtcggctct tatcacctgc
      961 cctactattt ccctcacact cagattccgc ttaaaaccga gcgttcctgt ccggcagaaa
     1021 gaacatttca aaggacaacc tgcctgagtt gaaatacaca gagtattcct cttatcttca
     1081 ggaattatga cagattcgat aacagctccg tcttcaagag tgaatgtcag tttttttgtt
     1141 ccgtcatcag atgaatgtat tgccggctca gaaattggag ttatcgaaaa aagtttctga
     1201 aaagcgaccc tgtcatcttt gcccagatcg gtcatttctt caaatgaata gacatctctg
     1261 tgatatatcc atcttattat ctgtcttgcg cggaattttg gataacctgc tgattctatt
     1321 gcattttcaa atgtctgtct tgtgaattca agaatttcca tatcagagtt tcacaaaaga
     1381 attccgtgtt ctttcccacg caatcgtgct tgttccctca tgtcccggat atatcacagt
     1441 cttacctgga agaacataaa gcttttcctt tatatttttt acaagtgaaa ttccgtctcc
     1501 gcccgggaga tcggttctcc ccacacttga tttaaaaagg gtgtcccccg aaaaaagatc
     1561 gtcttcaatg tggtagcaga cacatcccgg agtgtgtcca ggagtatgaa tgacttttat
     1621 tgaatgccct gcaaattcaa gctcgtctcc gtctttaagc tcaatgtcga ctttgcctga
     1681 atatgcaaaa tcaatataaa atccgataga taaattcttt ttcgggtcgt aaagccactc
     1741 tttttcaaga gaatgtacag caagcaatgc atcgggaaat tgctttttaa tatcgttcaa
     1801 agcaccgata tggtcataat gggtatgggt cagaaggata tatttaaccg atttaccttg
     1861 tgcttcttta attatgtcag cactgtcacc gcccggatca acgatgaacg cttcattatt
     1921 ttccgaaaca atataacaat ttgtcccgat cggagcaacc tgaagaacct ttatttccat
     1981 ataaacccct tgttttattt atgaactctg ttgaatgcta caagaacacc gcgaagtttg
     2041 tcaatgtgat tattagtcag atccccttct ttaaatctcc atgaccagtt ctgaggaccg
     2101 gtagttcccg gaacattgaa acggcagtca ctcccccacc ccagaacatc ctgaagagga
     2161 aaaaaagcaa gatcagcgat cgaagaaagg gcaagacgga tcattttcca gtgaatatca
     2221 tccccgtttg aaccgagata gtggttcaca taattccttg tatcactgct ctggctccag
     2281 taccagcccg cagttgtgtc attatcgtga gttccggtgt agatggcaca gttagtgtct
     2341 ttaaaattat gcggcaggaa ctcatgatct gtcccgtcat aaaaagcgaa atgaagcact
     2401 ttcatcccgg caaaaccgaa ctcgtctcta agttttataa ctttttcatc aatatttcca
     2461 agatcttccg caataacagg gaaatgaccg agttctttta tcactgcatt gaaaagttct
     2521 tttcccggag ttttcttcca gacaccgttc tttgctgtat tttcagaaga cggaactgac
     2581 cagtattcgc tgaagcccct gaaatgatca agccggatcg aatcatacat tttaacatta
     2641 tgggaaaatc tttcgatcca ccacctgaaa tcatctttct tcaatttatc ccagtcataa
     2701 agaggattac cccataactg accgttttca ctgaaataat ccggtggaac acccgaaact
     2761 tcaagcagtt cgccctcaac catcttgaaa atatgctgat tcgcccacac atcggcactg
     2821 tcaaacgaaa gaaaaatagg aatgtctccc attatttcga tacctttgct gtttgcatac
     2881 gatttcaact ttttccactg gctgaaaaat atccactggc cgaactgata tctgttgatc
     2941 tcggttttat gttcagatgc aaattttttc aaagcagaga catctctttt cctgtattct
     3001 tcaggccaga gtttccacga tcttccttca aatttatcgg taagtgtttt gaaaagtgca
     3061 taatcatcaa gccactgagc gtgatccttt ttgaagatct caaactcacc ggattctttt
     3121 tttatcccgg caaattctct ggccgctgaa gcaataagct tgtttttaaa gtcaattact
     3181 tttctaaaat caaccctgtg atcgttaaac tgaggtattt caacatcttc cggaacaaga
     3241 cccgatttca gaagatcttc aatatctatc agaagttcat tgccggcgaa agcactgtat
     3301 gaattatacg gacatccgtc aatccctgtc ggggtcaaag gcagtatctg ccatattctg
     3361 atccctgcat cagccatcat atcaatgaat ttaagagcat tgccgcccat atcaccgaca
     3421 ccccatttcc cgggaagaga tgtggggtga agaattattc ctgctttacg gttgtttgtt
     3481 tttttattaa gcggcatcta agctcctgat caaagacagt tcaaaagacc gttcttttcg
     3541 ataatatcct tctgaacagt ggagaatttt ttgaaatgac ctgcaatttt tccgttgtta
     3601 aatatttcac ttgtctcaat atcaaattca agtttcgtcc cggtctcaag aagtttttca
     3661 tcaatatcgg ttatctctat catcggaaag ccggtgttaa tgatatttct tttgtagata
     3721 gagccgaacg atttcgccgc gacaccctgc ataccgagtg aaatgaaaca gtcaaccgcc
     3781 tgctgtctgc tgcttcccga accgaaattc tcaccggcga caagaaccat ccccgactta
     3841 actttctttg caaaatcctt atagccatca agattgtcaa gagcatactg tcccatgagc
     3901 tttatatcga caattgaaag atacttgttg tgaaatatct gatctgtatc tatgtcgttt
     3961 atcagatttc cggatccgtc tttcagaaca aagatctctg cagatattct tttcatcatt
     4021 ttttctcctt cttaagtaaa ttatctataa ctgccttaaa atactctttt ttctttgcac
     4081 cagaaaccat ctttccgtta ataaaaaatg ccgggacacc ccttatccca tgttttgaac
     4141 cctcttccat gtcctgtttg agaatgagat ctgtttcggg actttccatc attttggata
     4201 tttttttcca atccaatcca agttcctttg aataactctc aaattttttc atatggtcgc
     4261 ttttccactc actctgctta tcaaaaagca tctttccaag agcgaaaaac ttttcatttc
     4321 cgtagatctc cttaactgca atagagaatt ttgcggccgg tttcgcctcc ttgtggaaag
     4381 caagcggcat atgtttgaac acaagccgga cctctccttt gtattcatca acaacttctt
     4441 caatggttga agcacctctc cggcagaaag gacactggaa ttcagaaaac tccactattg
     4501 taattcccgc atcttttttc ccttttaccg gttctttgcc cgtgaaagac agctcgtata
     4561 ttttgtccgg gtcacttgcc ggcttttctt tacctgccga tctggatata ccgggttttc
     4621 cgtctttaat catctcatta taaagatcat tccctttgag tcctttttcc ctcagaggtt
     4681 gaactttttt cagctcttca tctataacct gtttgaatgt ttcaaacgga agcgcaccgt
     4741 tcacgaaccg tccatttatg aatgaagcgg gagttcctct gacaccgagc ttttctgcaa
     4801 gagcagagtc atcttccacc attttcttca actggggaga ctccatatcc tttttgaatt
     4861 tctcaatgtc aagttcaaga agctttgcct cgtcttcaaa ccatttctga agatcgggag
     4921 catccttcca ttccttctgt ttcttaaaga gcgaatcata catgtcccag aacttttcct
     4981 gcttatgagc ggcaagtgcc gcaagtgccg cgggtgtcgc ctttttatga aaacggagcg
     5041 gaagattgac aaaaaccacc cgtatctttc cttcgtattc ttttttgatc tgttcataag
     5101 tagtatgcag tcttgaacag aaagggcact caaaatcatc aaacagtatt atggttacat
     5161 ctgcatcatc aacaccccat acaggagaat gttcgggaat tccgacttta taaacatcag
     5221 aagggacatt gccgggatcc cttttcggtg gagtgtatct tatctggccg tctttaacta
     5281 tctcggaata tacatctttc acacccttgg ctttaatttt ttcaccttca gatatctgtt
     5341 tgacaagcag ctctgtgatc tttacattat tcgcgcccac tacccttctg ccgttcacaa
     5401 acattgtcgg agttcccctg actccgaagg tcatcccttg cgaaatgtca ttctggagta
     5461 tctttcccgc ttcttccgaa tttctgtctt gctcaaattt ttccatatca agaccgaggt
     5521 cagatgccca tttgaccaga ttctcatcat taatattttt tatgtcatca aataatctta
     5581 aatagtattc ccagaatttt ccctgtttct gtgcggcaat ggaggcaagc gccgcgggct
     5641 tggcaagttt atgaaaaccg agagggaaat gtttgaacac atatctcacc ttgccgtcat
     5701 aatctttcat tatcttcgac atcaggtcca ctgatctttt tgagaaaggg cattgaaaat
     5761 cggaaaagtt tattattgtt acaggagcat cggcaggacc ctgaaacatc gatgttccta
     5821 tctcaacttt aaatatttca tcatcctgtc tggcttcggc actttctttt tctgttttct
     5881 tgtcaacttc atttttccct gtacacgaaa acataaaaaa tgcagttagt gcaaaaagga
     5941 tggtgatgca tacttttacc tttttcattc cgaacctctc tataaaacag acaaaaataa
     6001 cttgtaaacc aaaaatgtac cgatcataga atagataccc gccgcaacat catcaagcat
     6061 cattccccat ccgttatgga cagatccgtc aaaataagat gccggccacg gtttccatac
     6121 atcaaaaagc cggaaaagcc cgaaagatga aagaacaaca acccacgaat taagcgaaaa
     6181 gagatttaaa tattctgacg gaacaaggag cattgatatt gccattccgc aaacctcatc
     6241 aatgacaacg atctgcggat catctgccga gctgtctgtg cttatatatt gcgaaacagc
     6301 cacacccgta ataaatgtaa caactgcgaa gatcagaagc ggaaaaaagc cgagccctgc
     6361 gaaaaaccac acaaaaggca atgccgcaag ggagccggca gtcccgggag ctaccggtga
     6421 atatcccgat ccgaaccatg ttgcccatgc atagtaaatt aacctcaatt actgctcctt
     6481 cgatgcctcg tattcttcta cgatcttctt ctgtacatca cccggaaccg gctcataatg
     6541 atcaaaggag atcgtgtatg tgcctcttcc gtttgtcatc gaacggagct gcgttgaata
     6601 accgtgaatt tccttaagag gaacaagcgc cctgactatt gtatattttc cttcgctttc
     6661 cattccctgc ggttttccgc ggcggctgga aatgtcgccc attacagagc cggcaaattc
     6721 ttcaggaact tttacaagca tgttatatat cggctcaagt attatcggat ttgccctttt
     6781 tgcagcatct ctgaaagcca tctgcgccgc tttcttaaag gcaacttcct tactgtcaac
     6841 cggatgttcc tttccgtcgt tcagagtgac tttgcaattt atgaacttgc atcccgaaag
     6901 aacgccttca ggaaggatct cgcgaatccc tttttcaacg gctggtatga acctgtttga
     6961 aattactccg ccgactatag catctacgaa ctcgaattct gcttcaggtg tcggctcaag
     7021 aacgatattg acctctgcaa attcacctga accgccagac tgttttttat gacggtagcg
     7081 ggcttccgct ctgcctttta ttgtttcgcg gtagggaact ctcggctcca ctatcttgac
     7141 atcaagtccg aactggttct ttatcttctt tgaaattacc tcaagatgaa gttctccgag
     7201 accgtcaaca aaaagctgtt tcagttccgc atcctgaata aacttgaagg aagcgtcttc
     7261 cacctgcatt ttcttaatgg cggcggcaac tttatcggta tcttcctttg aaacacccat
     7321 aaacgcgcct ctgactattg cttcaggcca ttcaataagt ttgaacggga aaatttctgc
     7381 attcttgttg tcggcgaact gatcacctgt ttttgtgttt ttaagtttaa cggtggcggc
     7441 tatctgtcct gccgtaacag ttttaataga aacacgctct ttgcctctgg tataaaaaaa
     7501 ctgtccgaat ttttcatatg acgatgaatt tatgttgtaa atgtcagctc cttcagagat
     7561 ctctccctgc agaagtttaa aaatgaaaac ttcaccaaac tgcggaatag tgttaacttt
     7621 aaaaacgatc cctttgggtg atgtcttcgg attcatttca atctctcctt cagcaaattt
     7681 taacactttg ccttcaggag aagggaaaaa atcaacaacc gcatcaagaa gacagtctac
     7741 accgacaccc ttgtttgcag caccgaaaaa taccgggaat accgaaccgt ttgcgaaagc
     7801 ttttgcaaga cctgctgtta tttccgcatc acttaaagtc ccttcggaaa aatatttctc
     7861 cattaacgat tcttcgcttt cagcaacagc ttccataagc ttttccttaa gctgagccgc
     7921 tttatctgca aactctgccg gaatttcact ttccgatgct cttttaccgt ttgcatattt
     7981 aatgagcttt cctctgaaca catcaattat ggaatctacc gacatccctg agcccgcagg
     8041 aaaagttatc ggtgcaagcg ggatctcaag ctctttactt atactgtcaa aggcatcgtc
     8101 aagattaatg ttgtccctat ctatttcagt aacgaaaaaa gctatcgatt tatgaagttt
     8161 ctttgcagta ttaaatgcat tctgtgttcc tgcatcaaga ccggaagacc ctttaactgt
     8221 aaggactgca gcttcacaga aagggatcgt cgcagcaact tcacctgcaa aatcaaaata
     8281 tcccggagta tctacaaaag aaagcagaca ttttttgtat tcgcaaccat taactgaagc
     8341 tgaaattgaa atacctctgt tcttttcctc ttcggtaaaa tctgaaactg tatttccatc
     8401 ttcaaccgtc ccctgcctgt ttactgcacc tgcattgaac ataagagatt ccaccaaagt
     8461 tgtttttcct gaagttccat gtcctgcaac acaaatgttg cgaagagcgt cactggtaaa
     8521 agcagtcatt ttttttcctc cgtatcaact ggttttctta atataaagct catcgatctc
     8581 atttttcaca aagtcataca tctgtctatt tgatgcgatt attgtcattc cggtaatata
     8641 tctgtcattt ccataaaaat cggtcactat cccgcccgcc tctttaacta taagcgatcc
     8701 ggcggcaaca tcccatggag aaagacccgc ctcataaaag aagctgaacc ttccgcatgc
     8761 ggtatataca agatcggcaa ctgccgaacc gaggcgtctt aaagcccttg tttttttgat
     8821 tatactgttg aaaacagcca gcgtactttg atcactttcg tgtgtaatat ctgcaaaacc
     8881 ggttgcggcg acacagttta ttgctttgtc cgtatcactc acctttatct ttttcccgtt
     8941 aagaaaagct cccccgcctg atgctgcata gaaaagttca tctgtaacag gaagaaaaca
     9001 cactcccgcg tcaacatgcc tgccgttaaa gtgcgccact gaaacagcaa aaggggcgat
     9061 accgcttata aagtttgttg ttccgtcaat cgggtcaatt atccagcagg gatcgttaag
     9121 tgttgtagcc ggattgaact cttcgctcag aaaaggaata tcatctatcc ttttcaactt
     9181 ttcaaggagc attttctcaa ctaattcatc cgcctcggta acaagatccc tgtcggattt
     9241 gaatttttta cgaagacccg gaacattaaa cattgttttt gcaaaatctc ccgcttcttt
     9301 tacagttccg gcaatttcag ttagacggct attcgagata ttcactcatt ctctcccgcg
     9361 agttgagaag aacattcttt agatatgtct tttcttcttc ggaaagattc ccttttgtct
     9421 tttcttcaat catttcaaga agtccgacat tgaatctcgc catatcgaca ttttcgcaga
     9481 ccgaatcaat cccttttacc tttatttcac ccagattgaa aagtaccgct gagtaaattg
     9541 atgaaacaag gctctcaaat gaaggattaa caaccgagga aagatcactt ttcttacatt
     9601 tcttttccat gtttattcca gatgaaattt aatgttcctt ttttctgaca ccggcatctt
     9661 taagaagctg ctcttcgttt atttccacat gctcattcgc atcttcaagc gactcaagat
     9721 attttttata atccttttca gttatttcac cgcttttaag ttttctttca acaaccctta
     9781 catcaaattt tgaaaaatcc atcttaaact cctgaaaaca ttaaattatt tgaaattaat
     9841 ctctaacgat gccccgcttt cctttccctg acttgcggtc actgcaaaat atgtgccgac
     9901 acctgctccg ccgagcacaa caactccgag aagtgtccac agccaccact gtttgtgaat
     9961 gggtgtttta ggtcctttat ccttcttatc tttctttgtg tccaaaccca gaagttccgc
    10021 ttcatcaaga gccacatccg atattggttt gaatccgtat gtgtcattta taaggtcctt
    10081 gttgaatttc tgaagctcat ccacttcagt aagatctttc ctgtcgggaa gaagaaaaga
    10141 tgtctcaact ttcttaaaat tccgtttttc ccgattcaca atatgacctg agtatttaac
    10201 cgtttcaccg tcaactgtca gacttgtcac aagcaccgaa tcaactttca tcttttctgc
    10261 gatctcgaca cactttctga gcattgcaag acggccgtac tctttaacca tgaatgattc
    10321 tgcttcgtgt aaaaatcccg cattgtcata ttttgaaagg tcttcggcta tcttggactc
    10381 ttttctttca cggaccgtaa atgtaccggc acggtcacga taaccgttct tatggatcct
    10441 gtaataatgt cttccttcag tgatgccgtg aacttttgtc ggggtaatac cgactatttt
    10501 gccattgatg gacacaagag ccccttcagg aaaactttca actctgacag atccgttcgg
    10561 aagcatcaga aaatcttcct tgatcctgtc aaaaaatgaa atgatctccg gtgaaaaaac
    10621 aatatcatca agttccgcat cgggataaat gcttaaataa cttgagaaaa gcggtgtcgc
    10681 ttcatcatct tcgtcaagca ttttgtaact tgcgccaaga taaagaagaa tgtctccgag
    10741 cactttcata tcaggaatct tatctaaatg cttttctatt gtctttcttg cagactgaaa
    10801 tttcgttatg gcttcttcga tgctgagatc attatataga gcttttgcct cttcaaatgt
    10861 cttcaatccc tcctcaagat ctctttttgc aaccgacatt gacggaacga acaaagccgc
    10921 ttccgttttc aggtaatcat attctgaacc cgcaagataa gatgctgttt ccgcaacaat
    10981 attccctgaa cttttctgcg aaaaagagtc cgggaaaagg taaaaggccg caattttctg
    11041 ttttcccgag tcttctgaaa aagatccttc tgaatcttcc atatctgatt cagctgaatc
    11101 ttcagcatca tcaaatgaat aatcactctt tttcgaatct ttcccgggct tttcaacttc
    11161 ttcctccggc tctttcacat cttcctcatc ttcaagaccg aggtcgagct catccagttc
    11221 gaagtcatca tcagcacgga gctgtgccaa tgtgagtcct gaaggagtca aggaaagaaa
    11281 actgaaggaa caaagaagca ggtaagtgac taatgatctc attatcttta ctccgaaact
    11341 aaataaccaa aaaattctga ggtatagcta tacttaaaaa gggctctgct gtcaaatttt
    11401 acccatttga attgcaaaga ataaaaaaaa cattataatt tttgagttat ttagtttatt
    11461 tacttcttta agttggattt tatttatgca gtttcaaacc gattttgaca tttgtttttc
    11521 cgttttggag aactccggta tccgtgccga tcttttcctg agttcactgc ttaatatttc
    11581 aaggtcaaga atacagaaga acatcgcact ggagaacctg tcagtcaatg gaaaagtaat
    11641 aaaaaagaat agttacagcc gttttcaaaa ggggatggtc gttgaatttg agatcgagcc
    11701 ggttgaacag atatgtgcag aacccgagaa aatggacctt gacatcgttt ttgagaacga
    11761 tcttttcctc attgtgaaca agccggcagg gttagttgtc caccccggca caggaaacta
    11821 ttgttcaact cttgtaaacg gaataatcca tcatctgaac tggagtggtg atagattaaa
    11881 ggaaggtgat ccggtaaggc ccggacttgt tcacagaata gacaagaaca cttcaggcct
    11941 gctggtcatt gcaaaaactc aggcctcgtt cgaagagctt tccttaaaaa tgtccgtcca
    12001 tgatattaca agggagtatg tgtgtattgt acgcggtcat ttgaaacaga acaaggggac
    12061 catagacaca tttcacggca gagatccatc caaccgtctg agattctctc caaatgtaaa
    12121 aaatgggaaa agggctgtta ctcactacga gataactgct gaatataaat actgttctct
    12181 gctgaaagtt acgcttgaaa cgggccgtac acaccagata agaatgcaca tgagccatat
    12241 aggacatcct gttgccaacg atctccttta cggcggcctc gtaaaaactg atgatcccgt
    12301 gttgaataaa aagcttaatt catgcccccg gcaacttctg catgcaaaaa ccctgggatt
    12361 cgatctttgc ggacagaact atctttttga gcatgaaata gctgatgaca tgaaaggaat
    12421 aataacatat cttgaaagta tctccggagg ggaaaaatga aggaaaaatc actgcttctg
    12481 tttgaaacaa aagagctgac tgctcaaaaa tactttgatt ttctcaagga cactccctgg
    12541 gagatatatc acaaacagaa tcttgaaaca tttctggaaa ctctccagaa cgatacattc
    12601 gatctcataa ttgtagaaga atcgatggtt cctgaagaag ttgtggctct tctcgctgaa
    12661 tccgacactc cggtcattct ttcaactaac tcctcaaaaa cagggcgatt gacaacgatc
    12721 ttaagagact tttcctcagc agacctcatc aacgcaatag gaaaatcaag ttttctgaag
    12781 atcgtgcgac agaaagacgg tgtttcttat gaagaacctg agcacgacga tgaagatgaa
    12841 ccggtactgc ttgaacccgt atttgaggaa aactccctcg atgcagttgt tctgacaccg
    12901 gaaaaaggtg acggaaaaac aaaaagctcc tgggaagtgc ctgaaaaaaa gggaactgag
    12961 agcgagccgg tcttagagaa aaaacctgag gagaaaaaag atatttttgc aagaattgac
    13021 gagatcgact ctattctttc aagtttaagc agagacattg aaacacagaa acctgaaccg
    13081 aaaacatctg caaaacccgg attccaattc ggagatgctg atgatgatgc gcaacaggaa
    13141 aaaactctct ttgcagtaca gccggatgtt aaagaagagg tttcaagaac agcagaaaat
    13201 aaaaatgaaa atgctgataa aaatgaagat aaaaatgccg attttctatt tgatgacgat
    13261 tacaaatatg acgcaaaagc cgttgagccg gataaaaaac cggcggttca aagaccggaa
    13321 tttgataata atatggttaa tgattttgaa gcaatacttt ccgacaaacc gcttaatgtt
    13381 ccgaatgtga aagccgaaga gtttgagatt gagacacatg atgaaaagat cagggaacct
    13441 gaaccgtcag aagctgaaag ttcttcaaat gtggaaaaga aagcggctca aactgaggac
    13501 aaaactctgc tcaatgaaga tattacaaaa gaagagatca gaaactggct tgaaaagaac
    13561 ggccggcaga taattaagga aatcgttctt gaacagcttt caaaactttc gggcaaagat
    13621 ggttaaaaag atagtttctc ttgatgtggg aatcaaatat gtcggtgtcg catcaaccga
    13681 ccctctctgg aatttccctg ttattttagg aactttgcaa agaaaacaat cgattgccga
    13741 tgatcttgca aggttatcgg tcatgctcaa agatattgat gtctgcggag ttgtgatagg
    13801 atttcctctg acaatggaag gacatgaagg gtctttcgca cctgttgtga aaggatttga
    13861 aagaaggttg aaggagcttt ttcccggcat tccttttttc agaatcgatg aatcgctctc
    13921 ttcaaaagaa gcagttgaaa ttaagcagat aaaacccgga aactacagaa aaaacaaaaa
    13981 acagggaaaa attgattcca ctgccgccgc cataatactt gaacggttca tgggaacaca
    14041 tgagtttgaa aatcttaaat tttcggttct gagaacagga gaatgatcat gaaaacagga
    14101 attcttaaaa tatcgggcat attctgcggg ggctgcactt atgccattga aaaagcggga
    14161 agacggcttg aaggtgtcag cgaagtcaga gtgaatcccg gaaccggaga gatcactgta
    14221 aactatgacg gcggaccgga agtgcttgaa aaaattgtca gaataattga aactttaggc
    14281 cacgagtctg aaatagctga acttgacagg tcacattaaa cacgaccgcc cttttctata
    14341 tacatatcaa acggtatttt ggcatccatg aaaccttctt cgttgaaaac cattaccctg
    14401 tttgcaagcg tttcgatgaa ctgatggtcc tgtgaaacaa acaggatcgt tcctgtaaag
    14461 ttgacgagcc ctttgttcac agcggtgatc gattcaaggt caaggtgatt ggtcggttcg
    14521 tcaagtatca gcacatttga agttgaaagc atcattttag aaagaacaca tcgcattttc
    14581 tctccgccgg atagaactga acagcttttc agcgcatcat cgcctttaaa aagcattctg
    14641 ccgaggaaac tccttataaa attctcactt ttctcctcag aatactgtct gagccagtca
    14701 ataagatcaa ggtcggaatt taaaaaataa cttgagatgt ctttcggcat gtatgcaaaa
    14761 gagaccgtag gcccccacct gtaatcacct tcatcaggtt tcaattcacc tgcaagtatc
    14821 ttaaaaagca cagtttttgc catatcatca gagccgacga gagcaacttt gtcgtttttt
    14881 tcaagccgga aactgatgtt cttgaaatat gttactccgt caatagtttt tgacagattc
    14941 tcgaccgtca gaagctcttt cccgatctct cttgcgggct taaaatcgac atatggaaat
    15001 cttctggaac tcacctgcat gtcttcaagt gtcaactttt cgagctcttt ctttcttgat
    15061 gtcgcctgct ttgcttttga agcattagaa ctgaatctct caataaactg cttgagttcc
    15121 gctcttttat cctctatctt cctgttctgt tcttttatca ttttctgcat aagctggttg
    15181 gattcatacc agaaatcgta gttccctaca tacattttga tctttttgaa atcaatatcc
    15241 gcgatgtgag tgcatacttt gttcagaaaa tgtctgtcat gcgaaacaac tatcactgtg
    15301 ttttcaaaat ttatgagaaa attctcaagc cacataattg acctgatatc aaggtaattt
    15361 gtcggctcgt caagaagaag gattcccggt ttgccaaaaa gcgctctcgc aagaaggact
    15421 ctcactttct ctccaccgtc gagcactttc attttttttt tgtgataatc ttcggttatt
    15481 ccaagtcctg caagaatggt cgcggcatca gattcagctt cccatccccc aagtgctgaa
    15541 aatttttcct caagttcaga agcgagcaga ccgtcagctt cagaaaaatc ctccttttca
    15601 tatattgcat ctttttcaag catcgttttc cacaaaagct catgacctct gataaccgtt
    15661 tcaagaacct caaagtcgtc aaaagcaaag tgatcctggc ttaaaacagc aattctgcaa
    15721 cctttctgaa caacaacttc accactggtc ggctcaattg caccttccag tattttcagg
    15781 aatgttgact tgcctgaacc gtttgcaccg atgactccgt aacaattccc cggagtgaac
    15841 tttacattta catcatcaaa aagcttactc ccgccgaaag caagactgag cttgttagtg
    15901 cttatcatat aaatctcccg tattgatcca tggtcttact tacattttga cttttctttt
    15961 tcgtactgct cttttttctt atcaagcagt gcctgtcttt tttcgcgatt cttttctctt
    16021 ttttcaatca actgggttct tttaagctcg agatcgttct tttttttctc ctctttctgt
    16081 ttcatcatca gcagctgtcc gtcataacgg gagcttagcc cctctctctg agctctgtat
    16141 atctcttcat tattggcacg cctttcctgg atcgatcttt caaatgcatc tctgtctttt
    16201 cttgacttgc cgcggtcttc tgcgatctta tcattaactt ttataatttc cctttcaata
    16261 gcagaccttt ttttgagaag ctgctcaagc ggcttagtga acttgagatc aagagttgtc
    16321 ttataccttg tttccatact ctttttttct ctgtcccagg tctcctctgc cctttttctc
    16381 tgatttcttc tcaattcaat cttattgtcc ctgatctttg cggcagattt tatctgagca
    16441 tcattctgct ttttgaaatc gtgtttttcc attctgaatt tatcaagttc aaattccctc
    16501 tgttttttct cttcaagctt cttcttgcgg tattcaccaa gctccctgtt catttcctca
    16561 gatcttttgg caagttccat gtcaagttcc atgctttttg cttcaagttc ctcatctttg
    16621 aactttttgg caagttcttc tcttcttttt tcaatatcag attgaaaatc accaagctta
    16681 tttgccattg caacggaaaa atcctcaatt tccttatcta ctgcttctac aacggctatc
    16741 ttttcgacct cttttttctc ccaaacttca agctttgcat taaatccgtc gaatttcacc
    16801 tgatattcag cacttatttc tgcaagatat tttctgtcct catcttcgtt tgccattctt
    16861 atcttgtcga tatccacccc ttttgcatgc tgctccttga gataatttgc gtctatctct
    16921 tccttgacct tgtcaagatc cttgttcttt ttatctatat cttcaatttc tttagtcatt
    16981 tttgcgatct ttgcagtacc gtctgcaata ttcttttcga atttgctcac gaatgttttt
    17041 tctgccgcat caatgctttt tatctcttct tcatactgga ctttcgcctt ttcaagctct
    17101 ttttcctttt ccgaattgat cattttatcg agctttctca tgtcggcatc gacatttttc
    17161 tcaagatgcg taatggtttt ttcgagctca gttatctctt tttccaactg agcatcatct
    17221 tttttccaga cctctttcat ttcagtgatc attttcttaa aacttgcatc aacatccgcg
    17281 cagcttcttg cctcaactac agatggaaca gatacaaaaa cagcgatcag caacaaaatc
    17341 ttaaacattt ttcctcctgt ttcaaaaacg ccaacacaat atctatattc aaattgaaaa
    17401 agttttccat ttctttgttt agtgtgtaga atgccatccg gattttttga cggggatatt
    17461 atgcttctac tcagtgacat ttcaaaacag tatgcaggac agccgatatt aaaacatgtg
    17521 acgatacctc tttccaaaaa aaggactgct ttagtcggga tgaacggttc gggtaaatct
    17581 actctgatga agataatcgc agggatcgaa tatgccgata acggtgagat aaccacaaaa
    17641 agacacgcag ttgtagaata tcttcctcag caggcagttt acgatttcaa agggactgtt
    17701 tatgaagaag tttccgcatg gcttaattct gacaggttca acgagctttc agcccgtgaa
    17761 gctgagataa tccgcatact tgaaaatgat ttaaaccctg atgattcact ttgtgaggaa
    17821 cttgccgaag ttgttgagga acttcacact cttgatgtaa aaaagaaaag gaagaactct
    17881 gttgaaacaa ttctcttaaa tctcggtttt tcaaaagagg actgggatag acatgcaaaa
    17941 acattgagcg gcggatggca gatgaggatc gctctggcaa gagttctggt gagagaaccc
    18001 gacatcgtcc ttcttgacga gccgacaaac tatcttgata tcgcaacgat cgactttctt
    18061 gccgactgga tgatgaaatt tgaaggtcag gtccttcttg tttcgcacga cagggatttt
    18121 ctgaatcgcg tagttgaaga gacatgggaa gtttttgcag ggaatgtgag tatttaccgc
    18181 ggaaattatg acttttatct tgttgaaaag gagcaacgac tcagagttct tgaaaaacag
    18241 cgggaaaaac agctcgatga aataaagatt cttaatgatt tttatgaaaa gaaccggttc
    18301 aatgcggcaa cagctcccct tgcacagtca aaatataaac agatggaaaa actgaaagcg
    18361 gaactgataa cacttccgag aactccgtca aaaatgactt tccgtcttcc cgacccgaaa
    18421 agaggaggcg agatcgttgc tgaagttgac aaactctatc acagtttcgg tgatattgat
    18481 gtcattgccg actacaagcg gataatttca cgaagagaaa gaatcgcact ggtaggtcgt
    18541 aacggttttg gaaagtcaac tctgatgaac atcatcggtg gcgagatcga accattatcg
    18601 ggtgagtgcc ggcttggcaa ggatgtcgaa gtaagttatt tcagacagca tgagataaca
    18661 cttcttccac cggatatgag tgtcatacag ttcgttgaat ctattgcccc ttttgatctg
    18721 atgtcgcgag tcaagacact tcttggatgc tttcttttct atgaagatga ctgggataaa
    18781 aggatcggag ttttaagcgg cggagaaaaa gtccgtctcg ctttcataaa aatgatcatg
    18841 aaccccggaa acctgctgct gcttgacgag ccgacaaccc atctcgacat tgactcaaag
    18901 gagatacttt taaaaactct ccagaacatc gatgcgacaa tcgtttttgt ttcacacgac
    18961 tcatacttta tcaactctct tgccacaagt gtcgtatatt tcagaggaaa atgtgacatc
    19021 ataaatttcc ccggcactta caatgaatat cttgaccgct acggacacga cataattgac
    19081 gaagaaaaag caaaatccgg cggcactgat gcaccgttaa gtaaaggaaa aatggatttt
    19141 gcccagcaga aagagataag aaacaaaata aaccggctca aaaaagagat tgaaaacact
    19201 cagaaagaga tcagtgatct tgaaaaggaa aagaactccc tctcaaacca attaaactcc
    19261 agttcaggca atcagccgga aatctcaaaa aagataactg aaatagacag caaactcctg
    19321 aaactcatgg aacagtggga agaaaaaact gaagagatgg aacgattttc cgctgtgtga
    19381 atatattaaa aattaaaact cgtaacaacc catgtcaaaa ccggaaccgt ctggtcttgt
    19441 cttaccgtca agatcaatga cgggattttc tgcagcagtt cctgctttat caatgcattc
    19501 agagccggca ccgaggtgat aatcattggc ggctctgtca acaaacaaag gatccgcatt
    19561 tatgttgcct gttccggaat atccgccctg aaaaagagaa taagttacat ttgtcgctga
    19621 atttgtattg ttctgagtct gattggccgg tccgttaccg tgaacgatcg agctgtaaat
    19681 gtttgtcaca gcgctgtcat aattatgaat tcctgatccg gttgtgctgc tgctgccgtc
    19741 agttgtgttg tccgcaagag tcgagaaaat aagatttgca cttgaatcat ttctgacata
    19801 aagcccgcca ccgtaactct ttgcattatt ttttgttatc agagagttaa cgatcgttgc
    19861 tgaggcattg ttgtcaattc ctattgctcc tccgcggcat tctgtaccgc ctttgcagtt
    19921 gttgagcata aattctgact gtgttatcac tattgaattc tgtgatgtcg aaacagatat
    19981 tcctgccgca agagttgtag cttcattttc tgaaaaaaca cttccctcaa tcgttatgtt
    20041 tctggaatta ttcgacaaat gaatagctcc gcctgtcgct gcggtattct tggaaaaagt
    20101 acattctgaa aaagctgcac tttctacatt gttaaattcc actcctcctc cattctcact
    20161 tgcagaattt ccggtaaaaa cattatttga taaaacaagg ttcccccctt cagctctcaa
    20221 tgctccaccc cagttctgag tggtattccc ttcaaatgtg cagccggaaa tcgttaaact
    20281 tgcaggagca tacgcaaaaa tcgctccgcc tgaagcagtt ccgttttctc ttttggtaac
    20341 agtgtttgaa aaaaaagttg aatttgaaaa agtcacattt cccagataca caaagacagc
    20401 tccgccataa gccgaagcat tagtgctcaa tccccctggt ctttctccat tctgagcgtt
    20461 attaccttta aattcacaat cactgaaagt cacatctgat tcaataacag caaccgctcc
    20521 gcctctgcct tcatatccgt tatctgcaga agcccctaca gcaccgttgg aatcaaaaat
    20581 gacagaattt attacaaaat tcgatgcctg acttacatag agagcgcctg catagttgtc
    20641 tcctgttccg ttatttacat ttccaaaaaa tttaagagat ctgatgacca catttgaagc
    20701 acctgtcgca tcaattatgt tatccatttt gttgaccgat ccgtcaagaa ctgtgagcaa
    20761 cggatcattg tctcttttgt caccttctcc tgcaaaaccg ccaagtatcg aaatatttga
    20821 atagatcttg atcgttgcag ggatcacata tgttccctga gccacccata tctcacattc
    20881 atctatgccg ttactttcaa taagaacagc atcattcgct gagtcaactg cctcctggat
    20941 tgtcgaatat gcggtatgcc aggtcaaccc gtcaggagtc tttacacctg attctttaac
    21001 aaatctgaca cattgcgaac aatcatcacc ggcaaatcct ccctcgcagc aagacttaag
    21061 cgaatccgaa cagaaaaaat caccgcatgg ataagtgttt cctgcaacac attcgccgtt
    21121 attgttgcat gcatcttcac cgttgcagaa aacaccgtcg tcgcacaaag tagtttcagg
    21181 tctgttttca atctcgcata tcccggcttc ggaacaaatt ccttttttgc attctccatc
    21241 taaatataca caatcctcgt cctttgtgca atccttgatg acga
//
LOCUS       DAOOYB010000012        21024 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_2370_length_21024_cov_10.190710, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000012 DAOOYB010000000
VERSION     DAOOYB010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 21024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21024
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2370_length_21024_cov_10.190710"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..165
                     /locus_tag="PL056_01455"
                     /pseudo
     CDS             1..165
                     /locus_tag="PL056_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597331.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C biogenesis protein CycH"
     gene            397..1578
                     /gene="thiI"
                     /locus_tag="PL056_01460"
     CDS             397..1578
                     /gene="thiI"
                     /locus_tag="PL056_01460"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA U4 sulfurtransferase
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HPM45712.1"
                     /translation="MFRNILVRYDEIALKKRNRGWFEDLLLNNIRSSVGELGSAGKFR
                     GRMGVELKSEESLKEVVNRLSFIPGISSFSPAFKLPLSAPWDDIVECALKLAHEALSD
                     GRTVIKVICTRSNKRYPGTSIEIQQKLAESVLTNLEGKFTVSMKKYTFALEIEIDTNG
                     IFLFSRKIAGIKGLPSGCAGEYLSLLSGGIDSPVASFMTMRRGGRIHYISFYSPPYTG
                     EESMKKLEDLAGHLKKYQGVSTKLFIAPLIDIQLLIKARCHEGYRTILFRRMMFKIAE
                     KIAIANKFDALITGEALSQVASQTIENLTCINDAVKTLPVIRPLITSEKSDTIAIAEK
                     IGTFPISIRNCPDSCTAFLPKSPIIRGKLEKVLAEEKKLEPEISYLLEESVKNVRVVN
                     V"
     gene            1571..2428
                     /gene="lipA"
                     /locus_tag="PL056_01465"
     CDS             1571..2428
                     /gene="lipA"
                     /locus_tag="PL056_01465"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF004019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HPM45713.1"
                     /translation="MSEEMLKKSTVKSPFVAPVKKILRKYHLHSVCESAHCPNIGDCF
                     KKKTATFLILGNSCTRNCRFCNITHSDDIPLPDVHEPENLALAVRELNLSYVVITSVT
                     RDDLEDGGADHFFKVVEKVKEYSPQTLVEVLTPDFKGDEELLDIIAKSQIDVYNHNVE
                     TIYRLYEKVRPQADYFRSLEVLSYMKRKGFITKSGIMLGVGETVEEVRELMRDLRDAG
                     CDIMTIGQYFQPTKEHLEVEKDYTEAEFRMFEEYGLSIGFKEVYAGRFVRSSFNASEI
                     HKNVSGRVI"
     gene            2425..3816
                     /locus_tag="PL056_01470"
     CDS             2425..3816
                     /locus_tag="PL056_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45714.1"
                     /translation="MRKKLIIVILFFTFFQLSAVNFDPDESYNRFIKGHGVGFSIFPL
                     YEGDSTLLEFDLYGNFYFSMLRFGFLLPVRFIIYNNDNIETAGKIFPKEDWDDARDYV
                     SILTFFQYGFKSDLFYFYFGEHKNRYVRNGTILGGYFNNLKLRFPKRGVNIEVNTDYA
                     GLDFFMDDVTPPNVVGGRAYVKPVSFFSKQNYANNLEIGASYFADIFSPYNISIVSPG
                     GIPKRDIEKYTDHLFGFDISFRFVATKLYHMKFYSDINKIVDGGFGLHFGLEHEFLLP
                     FSTDMKILSRWEYRAMESNYIPSYFNTFYDTEREYYRNGQTKSRFVNDASREGLDWTH
                     GYYLDLVYDLTGTFAVGGSFEHNRIYKNNVNDGKFDNFQLNLFVNALIFNRIGTDFII
                     TFQDLGEKGKRMKDQPFYNLSVYYKLNEYLSLGFYTGSKWYLAYSGTSVATESYYKNT
                     AVYSVGAYGMMRF"
     gene            3916..6096
                     /locus_tag="PL056_01475"
     CDS             3916..6096
                     /locus_tag="PL056_01475"
                     /inference="COORDINATES: protein motif:HMM:NF012632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45715.1"
                     /translation="MKNTGALVFKCCIFLSALFFSCENELSQIEFDHSAKWKMVSPGG
                     NHVCAIDENDELYCWGSNLGGQLGDGTEKESLRPKKVNDYKWSEVSAGELFTCGIRVD
                     GDLFCWGTNYYGETGTGSDKEFINKPSKVGNMKWKKVKLVNMLMKTACALSFEDELYC
                     WGRNYPVTFGDISGESANVPQKAGDYKWKDFAIGTFGGCGINLENELFCWGQGQYPVV
                     DPESETRDIPNKIGNEKWNEISVFSFVHSDQTCAIRSDSTLFCWGTGTGIFSGLDSMK
                     EISSGWVDVSVGQYDVCGVKTDGFLYCFGKNMNGLFFGGGRDYAEEPVRIDSGKWKTV
                     KSGLTYKCAISEDEGLYCWGYNDLGQIGNGKKGNLEYPDKIDDEEWELISSGYSNNCG
                     IKDGKIYCWGNNSTGQLGDGTYNDRQMPVKTGNKNWKTVSAKGSATFAIDGDNKLYFW
                     GNSYENYVSDIFEQKNYTEPVIVDDGSWRQISTGHNHTCGIRSDNHLYCWGYNGDGQL
                     GFETFYYNGEYGKDKPEKVNDEEWMSVYAGDSQTCALNKENLLFCWGHSHYGCDSYEP
                     NFLIRQVSYMKWKKIDLMRDTYCGITEEDDLYCWGDNGAGQVGIGEINSSVICEPAKV
                     GNRKWKEVSTGFSGVCGIDSEDDLYCWGQNRYGFLGIEVVNDKYYSEIPVKVGRKKWK
                     SVSLGYESVCALDDSGKPYCWGNNFTGALGNNLAWVEEKFRVLR"
     gene            6265..6552
                     /gene="groES"
                     /locus_tag="PL056_01480"
     CDS             6265..6552
                     /gene="groES"
                     /locus_tag="PL056_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018123706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HPM45716.1"
                     /translation="MKLRPLNDRVIVKRVESETKTAGGIIIPDTAKEKPVEAEVVAVG
                     IGKLMDNGERQKMQVKVGDKILFGKYSGTEVKINGEEHLILKEDDVLAVVE"
     gene            6582..8225
                     /gene="groL"
                     /locus_tag="PL056_01485"
     CDS             6582..8225
                     /gene="groL"
                     /locus_tag="PL056_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528688.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HPM45717.1"
                     /translation="MSAKQIYFSHDAKSKILKGVNKLADTVKITLGPKGRNVAIEKSF
                     GAPKITKDGVTVAKEIELEDKIENIGAQLLKEVASKTSDVAGDGTTTATVLAQSIVSD
                     GFKYVTAGYSPVMLKRGIDKAVAQVVEKLDSITKKISTFDEIASVGSISANNDKEIGK
                     IIAEAMDKVGKEGVITVEEAKGMETYLETVEGMQFDRGYLSPYFVTNAEKMQVIMENP
                     FILVYDKKITNLKDLVPVLEGVAQQGRPLLIIAEDVEGEALATLVVNKLRGVLNIAAV
                     KAPGFGDRRKAMLEDIAILTGGQFISEEIGNKLENTTVQMLGRAKRIVIDKDNTTIVD
                     GAGKEEDIKARVKQIKAQIEETTSDYDKEKLQERLAKLAGGVAVINVGAATEVEMKEK
                     KDRVDDALHATRAAVEEGIVAGGGTCLLRCKDVLKKVKTENEEEKVGVEIIAKALEAP
                     IRQIAANAGVDGSIVVNTVAKNKDEEWGFDAAKGEYKNMIEGGIIDPTKVTKSALMNA
                     ASIASTLLTTEAVIIEAPKKDSAPAMPDMGGMGGMGGMM"
     gene            8360..9334
                     /gene="gspC"
                     /locus_tag="PL056_01490"
     CDS             8360..9334
                     /gene="gspC"
                     /locus_tag="PL056_01490"
                     /inference="COORDINATES: protein motif:HMM:NF041515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HPM45718.1"
                     /translation="MKSRTILNAGLFISITFAIASTVGFLLSFIVKDEQVFVPIGKSY
                     YRNGLEEDTEAKKESFYFGSNVEKVSSSNIFDAAMRNITVADNKVGETTGDGAVPEAS
                     ADDIYFYFENPEKCPLMSSLLLSGTIVAKEFDQSIAILREGSSREEGAIIARVGSEVT
                     PGVKLALVWRNFVVLTTSSGVKCIGEGVGGDAKQSVPDQRFAQTGEPPVAGNDGIEVR
                     KVGENQYVIRRQDLQKVTGNLNSLARQARIVPSKKDNGFKVFSITKDSLYSKIGIQNG
                     DVIKSINGIELSSPDKALEAYSRLQSASKLSLDIVRRGQNETMEYTIE"
     gene            9344..11875
                     /gene="gspD"
                     /locus_tag="PL056_01495"
     CDS             9344..11875
                     /gene="gspD"
                     /locus_tag="PL056_01495"
                     /inference="COORDINATES: protein motif:HMM:TIGR02517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HPM45719.1"
                     /translation="MTSKKILSLMLLLFLAFYGYAADGDDDVKVPVNLNNRLKGINQN
                     FSPKFSPKLDSAKKIKKKQKDGKEEKEENSENIQNPSEKPGVKTVTPPAPKTILDKKS
                     EDDPDASKLSDAVKIEVAETKPVDPNMKIKLDFKEEDLINIIKLFSELMQMNFIIPEN
                     LGKAKVNMMAPQKVTVREAYRTFLTLLAVNDFSVSEDGKFTIITREKNIPEMKIPFYK
                     GTDVPDLFKMVATIMKFNYVTAADMDKVLKLFRDKGATSLVFDDKTLIVVDYAANIRK
                     IMMLVKELDQPSESDSAKLYFIKLTHVLAADARKILEDIFKDFSKKGTKKKSAGSDNS
                     PNLAGVANPQRLPGSSRNPADPPSGDEESLSGENMYLHIVADERSQQLIVLCTQTTYN
                     LIIQIVQNIDREVEGEGEIHVVKLQNAKAEDLVKTLNQLSKNKKSTGSKTTAKGTDVF
                     EGEVQISSNESTNSLVVVSSMQDFRNLKKVIEKLDIRRKQVFVEAAILEVKIDDSLEY
                     GNAYTAGGFEVNVAGEKIPLFFGKALDSKMSPGTVAGMVAPAISGTEGIPGLGLAGGI
                     PSIGVILNAAQNDSSVNVMSTPHLLTTDNEEAEISVGETIPFPSGNIISGTAGSTVTY
                     TREDVALKLKIKPQINESGYMTLEVNQEVTELGAPSQYGFTTTKRQAKTKINAENEQT
                     IVIGGLMKDSVTEAENKVPILGDIPVLGVFFKYKRTVKAKVNLLIILTPHIVESKEDF
                     ARILKRKMEERDEFARKFYGDHVSFEDNIYLDKKRGALLTVVKEVDNTNRKEDEELLR
                     IKSKKDEKSIMVTPDGEEEIISVPEGSLDREDDLVPEAVPEFDGE"
     gene            11877..13562
                     /locus_tag="PL056_01500"
     CDS             11877..13562
                     /locus_tag="PL056_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006002886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HPM45720.1"
                     /translation="MARKKIGEILIEKGHLDASQLSRALELKSQSDKKIGQILVSEGI
                     ITPEQVAEAFAEQTGIPLIPEDFFKKVDPSLLSDFPIGLARKFKAMPVFADTELHVAV
                     TDTDPLLFNQLFMLYRKNVKPYICSTSKINDLINHAYSKIDSNEESLNAEEFADMNDD
                     SDVAIPDLIDSNDEAPIIRFVNNTIAKAVKDKASDIHFESYEDEVIVRLRKDGKLYTV
                     QRVPKAAQAGLITRIKIMSRLNISEKRLPQDGNIKVKIAGNDVDFRVSTLPSIHGESV
                     VLRILDKRSQQLSLDESGFTKRDLERMRKIIRMKHGIFLVTGPTGSGKTTTLYSALTE
                     INSPDVKIITEEDPVEYQLKGVNQIHINSKIGLTFASGLRSILRQDPDIIMVGEIRDR
                     ETADIAINASLTGHLVFSTLHTNDAPTAFTRLIDMGIEPFLISSTVLGILAQRLVRKL
                     CPVCKEAYYPEPAILEELGLDPAEYANHPFYKAVGCDECAHTGYSGRTGIFELVIMDD
                     ELRRAVVNRLDASDIRKIASANGMLNLREDGAMKAIKGITSPDEVLLRTQEDN"
     gene            13562..14791
                     /locus_tag="PL056_01505"
     CDS             13562..14791
                     /locus_tag="PL056_01505"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HPM45721.1"
                     /translation="MPLFAYKIIDSSGKEKKGNIECPNRNAVKSKFLSEGYYITEIRE
                     QSSAQSFSFDSLKSILSFGRKKVPLDEVASMTRLLATLQKAKIPLVESIDAVAQQQEN
                     QMLKEALIIIKGKMNEGYSFSRSARDFPNIFDDLYCNMLAAGEESGATDRVLLRLAGF
                     MESQVDLRNKVKSAMTYPVILMFVAIIVVGVLFTVVIPKLAKMFEDVNMSLPLQTEIL
                     IWLSGFIGSWWWLIAIVIITAFVLFRRHIATEKGAFWWSGFLINMPLFGRVNRMVAVS
                     RFAKTLSTLLHSGVPILNAMDIVKKIIDNKVLEKAVATARENIKEGESIAVPLKRSGE
                     FPPIVIQMISIGEQSGDLEEMLDTLADSYEKEVRYTMDRLTSMLEPIMIVALSVVIGF
                     IVFAVVMPILKINDAVG"
     gene            14808..15239
                     /gene="gspG"
                     /locus_tag="PL056_01510"
     CDS             14808..15239
                     /gene="gspG"
                     /locus_tag="PL056_01510"
                     /inference="COORDINATES: protein motif:HMM:TIGR01710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HPM45722.1"
                     /translation="MFKKFFKALLEERAGVAVKGFSLLEIMVVITIMTMIMGAVGVGV
                     MSYLDKSKIKQARIDIATISNALDLYKTEFGKYPDSEDGLSRLVEEKILKEKKAPRDP
                     WGNDFIYIYPGSNNEDGFDLYSFGPDGKEGGNDDIVNWDTE"
     gene            15247..16062
                     /locus_tag="PL056_01515"
     CDS             15247..16062
                     /locus_tag="PL056_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HPM45723.1"
                     /translation="MKKNSGFSMIEMLAVVALVIVMAGISVAGVGRIGRYKASADLGT
                     FNSFLRFNFMRSVRNGEYIRIVIDMETGSYWSEKSETPFFLSTGEATEKKKEENDEMV
                     ERMENGMGTDPFKEGGSALGMESIMQKARLLSSEDLDNDDYFNYENFIPDRRSLKKIL
                     QPEFETVSNQKKISDGLVVTGFFAYHTPEIVTRDLITDKEMEKNVHIYIFPEGRIEPF
                     YFSLGEESDDGETSYSYITSDMFMNTKIRPGSFEEDIKDMKDLFEDKDEEGRS"
     gene            16059..16706
                     /locus_tag="PL056_01520"
     CDS             16059..16706
                     /locus_tag="PL056_01520"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPM45724.1"
                     /translation="MTVSKGFSLIEVLAATTILAGVLFSLSRIISGSYIYTSKIRNIY
                     LGTELARLKLHDIQEKIKEDGLPETEMEEEGDFDQREYSEFKWKYSIKRVFIPLPDIS
                     SSADDGTQTQAEEAAGMLSLAKGNIEDFFKERIRKLSLTVFWGRGDRDSEKVVFTIFL
                     TTDGAVKSFQQYQGGGGVPGQDPSQGRARKSDPGYNRFKTPLNNPPAGLPGTEQR"
     gene            16709..17362
                     /locus_tag="PL056_01525"
     CDS             16709..17362
                     /locus_tag="PL056_01525"
                     /inference="COORDINATES: protein motif:HMM:NF023044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspJ"
                     /protein_id="HPM45725.1"
                     /translation="MSRKGFSLIEVLVAMSITAILASSVYFSFSNVIAGRSKIKEITE
                     KERKTYFTLELIRNDIKNAFLTNNKGVPEETHKTIFKSEEDDPVSHLTFTTLNHVKMA
                     AGVKQCDQTEIEYYGENVEGENVLFRRESLWIDEFPEKGGNVYPVMSGFERLSFQFWK
                     ESSKEWVDQWNTESADNLNALPPKVKITMLVKEGESGEEDYIIETVVEIKMLRPLSF"
     gene            17369..18574
                     /locus_tag="PL056_01530"
     CDS             17369..18574
                     /locus_tag="PL056_01530"
                     /inference="COORDINATES: protein motif:HMM:NF015867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HPM45726.1"
                     /translation="MKKFFSNMFKSFNRPKRGVALLIVIVMISILLPIVTDMNYEAKT
                     EFDLAINYKRKAEAQQLAASAVNFAVVIFDLQKQIEGILKQFNMGQQIEIWDIIPFDT
                     ALLRGFVTAGPFVELEDVVTKKDVENPTGSALNDQEGDFSHAETGDPIFQFPGDFKVE
                     FTGEDDKINLNHLAYGTRSAVIKMLEALIEPEFYDFIFLENTSRREYVDRLELIQNLV
                     DWVDPGDDKFTDGTKFLSGGDEQSDYADFLPEYKVKNAKFDTIQEVMMVYGVDDLIYK
                     ILEPHITIYSTGKININKASPEMIEGIIRAYAVDKAMTVFYSEDAMRELLGKILAKRA
                     RDGFAKTVDFVQACKDEGIDMESDVEKIIDVSGRVYRIKATGIKEGVESWVEMVVDRE
                     GNIYYYREG"
     gene            18590..20077
                     /locus_tag="PL056_01535"
     CDS             18590..20077
                     /locus_tag="PL056_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45727.1"
                     /translation="MKFASCRVIAGKLLINVFSREYNKTVLESRTESSVENCAEIFKE
                     VDYIDLVIPGKEVLFFSRNYPPVKSSYIRKIIVQDIEVETPFRENDLVIDMKSFSGNG
                     ETSVFCAKKEAVKNILDLFDAVTRDKIRSIVPEEILLFKLPEKIDRALFIGDDYSMLV
                     LRNGQIIMNGGINDLKGDLRTCFSGEDPDSELNAWLSVAGDISVPEGLSDIEMLIRKR
                     VIAFMERVFGFFGPFTNSSGMTGVFINDDLVNGMGRLIEQIDHPVFPGSQSIVYEAYE
                     SLEIIPEIVDNDSSVSFATGEFAYKGGFSFLKKRIVTAVVLYFTALIMLVAGMQMRIS
                     YLDERIEKGEERTKTLMKDVIGKEMPSLRQALSIMNKTIKGETALADKKAVYPYSALY
                     IMEVTFPYFTFENSTIEVSELAIKEEGKIRVTGTSSTLEDINKFTGFLEQDPLIDEIN
                     RGQINTRNDKSSFSISFSYAGPKKDDTKGKKGKKKDSEKDPGEEI"
     gene            20074..20616
                     /locus_tag="PL056_01540"
     CDS             20074..20616
                     /locus_tag="PL056_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45728.1"
                     /translation="MKEKIMEMLNNLSERERTVLTFLITFMMVFMFGGIWFLMDMTVS
                     NRKDTISEQETMIQKMVSMKTLYQKAQIRNEAMKSSISTSSVNLNSDISTVRESTGIG
                     ISTLKELKPRKKGDISIERIELGFRDVSLEQTMAFLYGIENRGRYVFIDSITIKKRFS
                     MQNYDVSVVVAALKKEVGVE"
     gene            20609..>21024
                     /locus_tag="PL056_01545"
     CDS             20609..>21024
                     /locus_tag="PL056_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45729.1"
                     /translation="MNNRLFGKIKEILASGRAGSLLKQTLFINIAFFAGIWLGVKIFF
                     PDDFILSKINKELFVKDMGLTADDVGISILGNISFTDGTLTEKGVKVVTFHKLKFSPS
                     IFSILQGNISGEFSINDINNQGGDLTVSFETSQEPC"
BASE COUNT         6792 a         3790 c         4952 g         5490 t
ORIGIN      
        1 aaacacataa aaaacattta cgattcatct gaattggatg aaaaaagcac ttattccatt
       61 ttggaatata tgggaaacga cggaaaacag ttatataaaa caagatatta caatttggac
      121 atgattattt ctattggcta tcgtgttaat tctgtaaaag caacccgtta gaacagttgg
      181 taagacaggc aatcccaccc aaacatggag ttgtgctcaa aatcctcacg aagaaccttt
      241 caaaacagct aattcttgac attcataaat tcaatgaaca gtttccgcct gctgaaatca
      301 aggaattcac ccattcccag gacagattta ttgacaatat ctcttaaatc caattaaatg
      361 cagtgatgtt tttttaattt ttatggaagt ggataaatgt ttcgaaatat acttgtcaga
      421 tatgacgaaa ttgcattgaa aaaaaggaat cgcggctggt ttgaagatct tcttctgaac
      481 aacatccgtt caagtgtcgg tgaactgggt tctgccggaa aattcagggg aagaatgggt
      541 gtcgagctta aaagtgaaga gagtttgaag gaagttgtga accgactttc gttcattccg
      601 ggaatatcga gcttttcgcc tgctttcaaa ctgccgctca gtgcgccgtg ggatgatatt
      661 gtggaatgtg ctctcaagct tgcccacgaa gctttgtctg atggaagaac cgtcataaaa
      721 gtgatttgta cccgttcaaa taagagatat ccgggaactt cgatcgagat ccagcagaaa
      781 cttgccgagt cggttcttac aaacttggaa gggaagttta cagtttcaat gaaaaaatat
      841 acattcgcac ttgaaattga aatagatacg aacggaatat tccttttcag cagaaaaata
      901 gcagggatta aagggcttcc ttcaggctgt gcaggtgaat atttatcgct tctttccggc
      961 ggtattgaca gccccgttgc atcgttcatg acaatgagaa gagggggccg catccattac
     1021 atcagcttct attctccgcc ttacacaggt gaagagagca tgaaaaaact cgaggacctt
     1081 gcaggacatc tcaagaaata tcagggcgtt tcgacaaagc ttttcattgc tccgctcatt
     1141 gacattcagc ttcttatcaa agcaagatgc cacgaaggat acaggactat tctgtttaga
     1201 cggatgatgt tcaagatcgc tgaaaagatc gcaatcgcca ataaatttga tgctcttatc
     1261 acaggtgaag ccctttcgca ggttgcaagc cagacaattg aaaacctaac ctgcataaac
     1321 gatgccgtaa aaacgctccc tgtcataagg ccgctcataa cttcagaaaa aagcgacact
     1381 attgcgatcg ctgaaaaaat agggactttc ccgatatcaa tcaggaactg tcctgacagt
     1441 tgtacagctt ttctgccgaa atcaccgata atccgcggca aacttgaaaa agtgcttgca
     1501 gaggaaaaaa agcttgagcc ggaaatatct tatcttcttg aagaatctgt taaaaatgtg
     1561 agggtggtaa atgtctgaag agatgcttaa aaaatcaacg gtaaaatcac cttttgttgc
     1621 accggtgaaa aaaatattaa gaaaatatca tctccattcc gtctgtgaat cggcacactg
     1681 cccgaacata ggcgactgtt ttaaaaagaa gactgcaact tttcttattc ttggtaattc
     1741 ctgcacaagg aactgccgtt tctgcaatat aacgcactcc gacgatattc ctcttcctga
     1801 tgttcatgag cccgaaaatc tcgcattggc agtcagagag cttaatttaa gttatgtcgt
     1861 tatcacttct gtcacaaggg atgaccttga agacggtggt gcagaccatt tttttaaagt
     1921 tgtagaaaaa gtgaaggaat attccccgca gactctggtc gaagtgctta ctccggattt
     1981 taaaggtgat gaagaactgc ttgatattat tgcaaaatcg cagatagatg tttataatca
     2041 caatgttgag acgatatacc gtttatatga aaaagtgcgt ccgcaggcag attatttcag
     2101 gtcacttgaa gttctttcct acatgaaaag gaagggtttc ataacaaaat cggggataat
     2161 gctcggagtc ggtgaaacag ttgaagaggt cagagaactg atgcgggact taagagatgc
     2221 cggttgcgac ataatgacga tcgggcagta cttccagccg acaaaggaac atcttgaagt
     2281 cgaaaaggat tatacggaag cagagttcag aatgtttgaa gaatatggtt taagtatcgg
     2341 ttttaaagaa gtttacgcag gaagatttgt gagaagttca ttcaacgcaa gtgagataca
     2401 taagaatgtt tcggggaggg tgatttgaga aaaaaactca taattgtaat cctttttttc
     2461 actttttttc agctttccgc tgtcaatttc gatccggatg aaagttataa ccgcttcatc
     2521 aaagggcacg gagtcggttt ttcgatattt cctctttatg aaggagacag cactcttctt
     2581 gagttcgatc tttacggcaa cttctatttc agcatgctca gattcggatt ccttctgccg
     2641 gtgagattca taatttataa caacgataac attgagaccg caggaaagat atttccgaaa
     2701 gaggactggg acgatgcaag ggactatgtt tcgatactga cctttttcca gtacggtttc
     2761 aaaagcgatc ttttttattt ctatttcggt gagcataaaa acagatatgt gagaaatgga
     2821 acaatactag gcggttactt caacaatctc aagctgaggt tcccgaaaag gggagtcaac
     2881 atcgaagtca acaccgatta tgcagggctt gactttttca tggacgatgt aactcctccg
     2941 aatgttgtcg gaggaagagc gtatgtgaag ccggtaagtt ttttttcaaa acagaactat
     3001 gcaaacaatc ttgagatcgg tgcatcatat tttgccgaca tcttttctcc ctataatatt
     3061 tcgattgttt ctccaggcgg tattccaaaa agagatatcg aaaaatatac ggaccatctt
     3121 ttcggctttg acatctcgtt cagatttgtc gcaacaaagc tgtatcacat gaaattctat
     3181 agtgatatca ataaaatagt tgatggcggt tttggacttc acttcgggct tgagcacgag
     3241 tttctgctcc ctttttcaac agatatgaag atattaagca gatgggaata cagagcgatg
     3301 gaaagcaatt acatcccttc atatttcaac actttctatg atactgaaag ggaatattac
     3361 cgtaacggac agaccaaatc ccgttttgtg aacgatgctt caagagaagg gcttgactgg
     3421 actcacggct attatcttga tcttgtgtac gatctcaccg gcacttttgc ggtcggcggc
     3481 tcttttgaac acaatcgaat ttataaaaac aatgtaaatg acggaaaatt tgacaatttc
     3541 cagctcaacc tttttgtaaa cgccctcatt ttcaacagga tagggacaga tttcataatt
     3601 actttccagg acctcggtga aaaagggaaa cggatgaaag atcaaccttt ctacaacctt
     3661 tcagtatatt ataagctcaa tgaatacttg agcttagggt tctataccgg ctccaagtgg
     3721 tatctcgcct attcaggaac aagcgtggca acggaatcat attacaaaaa caccgcagtc
     3781 tattcagtcg gtgcatacgg aatgatgaga ttttagcaga cagggtcttt tgaaatttta
     3841 acaacatttt gtatttttct attttttgaa tacaatgttt taacattact gagttttctg
     3901 aaatgtgagg tcaaaatgaa aaataccgga gcccttgttt ttaaatgctg tatttttttg
     3961 tctgctctgt ttttcagttg tgaaaatgag ttgtcacaga ttgaatttga ccattctgca
     4021 aaatggaaaa tggtttctcc aggagggaat catgtctgtg caattgatga aaatgatgaa
     4081 ctttactgct gggggagcaa cttaggagga caacttggtg atggaaccga aaaagagagt
     4141 ctcagaccga aaaaagtcaa tgattacaaa tggagtgagg tttctgcagg agaactcttt
     4201 acctgcggga taagagttga cggtgatctt ttctgctggg ggacaaatta ttatggtgaa
     4261 acaggaaccg gcagtgataa agagtttata aataagccgt caaaagtcgg aaatatgaaa
     4321 tggaaaaaag ttaagcttgt aaatatgttg atgaaaacag catgcgcact atcttttgaa
     4381 gatgaacttt actgctgggg caggaattat cctgtcactt tcggtgatat atcgggtgaa
     4441 tcagctaatg ttcctcaaaa agcaggtgat tacaaatgga aagactttgc gatcggtaca
     4501 ttcggcggtt gcggaataaa tcttgaaaat gaacttttct gctggggaca agggcaatat
     4561 ccggttgttg atcctgaaag tgaaacaaga gacataccga ataaaattgg aaatgaaaaa
     4621 tggaatgaaa tcagtgtctt ttcatttgta cattcagacc agacctgtgc gataagatcg
     4681 gattcaactc ttttctgctg gggaaccgga accggtattt tttcaggact tgacagtatg
     4741 aaggaaatca gttccggctg ggttgatgtg tctgtcggtc aatatgatgt atgtggtgtt
     4801 aaaaccgatg ggttccttta ctgtttcgga aaaaacatga atggactatt tttcggtggt
     4861 ggtagagatt atgctgaaga gccggtcaga attgattccg gaaaatggaa aacggtcaaa
     4921 tcgggtttaa cctacaagtg tgccatttca gaggatgaag ggctttactg ctggggttac
     4981 aatgaccttg gtcagatagg taacgggaaa aaggggaacc tggaatatcc agataaaatt
     5041 gatgatgaag aatgggaact tatctcttca gggtatagta ataactgcgg aataaaagat
     5101 ggaaaaatct actgctgggg aaataactcc accggtcagc ttggcgacgg gacatataat
     5161 gacaggcaga tgccggtgaa aacaggaaat aagaactgga aaaccgtatc ggctaaaggg
     5221 tcagcaactt ttgccattga cggggacaac aaactttatt tctggggaaa cagttacgaa
     5281 aattatgtat ctgatatctt tgaacagaaa aactatacag aacctgtcat tgtggatgac
     5341 gggagctggc gacaaatttc aaccggccac aaccacacct gcggaataag atcagataat
     5401 catctttact gctggggtta taacggagat ggtcagctcg gatttgaaac tttttattat
     5461 aatggcgaat atggaaaaga taagccggaa aaagtcaatg atgaagaatg gatgtcagtt
     5521 tatgccggtg atagtcagac ttgtgcactg aataaggaaa accttctttt ctgctgggga
     5581 cattctcatt atggttgtga ttcttatgag ccgaattttc tgataaggca ggttagttat
     5641 atgaaatgga aaaaaattga tctgatgcgt gatacatact gtggaataac tgaagaagat
     5701 gatctctact gctggggaga taatggtgcc ggccaggttg gaatcggaga gattaattca
     5761 tctgtcatct gcgaaccggc aaaagtcgga aaccggaaat ggaaggaagt ttcaacaggt
     5821 ttttcaggag tatgcggtat agacagtgaa gatgatctct actgctgggg acagaatcga
     5881 tacggttttc ttggaattga agtcgtaaat gacaaatatt attcagaaat tccagtaaag
     5941 gtcggaagaa agaaatggaa atcagtttca ctaggatacg agtctgtctg tgctcttgat
     6001 gattccggca aaccctactg ctggggaaat aattttaccg gagcacttgg aaacaatctg
     6061 gcatgggttg aagaaaagtt cagagtgttg agataattgg caaatgatct ggcatgaaaa
     6121 aagttgagca agtataaaaa aacggatgca aaaatcaata acggtcttga ctcacggttt
     6181 ttgtgcttat aaatatcgct gtttccggtt ttgatcaccg gaaaatgttt atttttactt
     6241 actttttata ttgtggaggt aacgatgaaa ttaagacctt tgaacgacag agtgatcgta
     6301 aaaagagttg aaagtgaaac taaaactgcc ggagggatca tcattcctga cactgcaaaa
     6361 gaaaaaccgg tagaggcgga agttgttgct gtgggtatcg gcaaactcat ggataacggt
     6421 gaaagacaga aaatgcaggt aaaggtcgga gacaagatcc tttttggcaa atacagcgga
     6481 accgaagtga agataaacgg tgaagagcat ctgattttaa aagaagatga tgttcttgca
     6541 gttgttgaat aaattcaaat agttataaaa ggagaaaaaa aatgagtgca aaacagatat
     6601 atttcagtca cgatgcaaaa tcaaaaatac tcaaaggcgt caacaaactt gctgacacag
     6661 taaagataac actcggaccg aaaggaagaa atgtcgcaat cgaaaaaagc ttcggtgctc
     6721 ccaagatcac aaaagacggt gtaacagttg caaaagagat cgaacttgaa gataaaattg
     6781 aaaatatcgg agcccagctt ttaaaagaag tcgcttcaaa gacaagcgat gttgcaggag
     6841 acggcacaac aacagctaca gtgcttgctc agtcaatagt aagcgacggt ttcaaatatg
     6901 ttacagccgg ctacagtccg gtaatgctca agagaggaat cgacaaagca gttgcccagg
     6961 ttgttgaaaa acttgattcg atcacaaaga agatctcgac ttttgatgaa attgcaagtg
     7021 taggaagcat ttcagcaaac aacgataaag agattggaaa gatcatcgct gaggcaatgg
     7081 acaaagtggg caaggaaggg gttatcacag ttgaagaggc aaaagggatg gaaacatacc
     7141 ttgaaacagt tgaaggtatg cagttcgacc gcggatacct cagcccatat ttcgtaacaa
     7201 atgctgagaa aatgcaggta attatggaaa acccgttcat acttgtatat gacaagaaga
     7261 taacaaatct caaagatctt gttccggtcc ttgaaggtgt cgctcagcag ggcagacctc
     7321 ttcttataat tgctgaagat gttgaaggcg aggcacttgc aactctcgtt gtgaacaaac
     7381 ttcgcggagt tctcaacatt gccgctgtta aagctcccgg attcggtgac agaagaaaag
     7441 caatgctcga agatatcgca attcttaccg gcggacagtt catcagcgaa gagatcggca
     7501 acaaacttga gaacacaact gttcagatgc ttggaagagc aaagagaata gtcattgaca
     7561 aggataacac aacgatcgtt gacggtgcag gaaaagaaga ggacatcaaa gcgagagtta
     7621 aacagataaa agcacagatc gaagagacaa catctgatta tgataaggaa aaacttcagg
     7681 aacggcttgc caaacttgcc ggcggagttg cagttatcaa tgtaggagcc gcaacagaag
     7741 tcgaaatgaa ggaaaagaag gacagagttg acgatgctct tcatgcgacc cgtgccgcag
     7801 ttgaagaagg aatagtagca ggtggcggca catgtctgct ccgttgcaag gatgttctca
     7861 agaaggtcaa gaccgaaaat gaagaagaga aagttggagt agagatcatc gcaaaagccc
     7921 ttgaggcacc catccgccag atagctgcaa atgcaggggt tgacggaagc atcgttgtca
     7981 acacagttgc gaaaaacaag gatgaagaat ggggatttga tgccgcaaaa ggtgaataca
     8041 agaatatgat cgaaggcgga atcatcgacc cgacaaaagt cacaaaaagt gcactcatga
     8101 acgccgcttc aatcgcttcg acacttctta caacagaagc ggtaataatc gaagctccca
     8161 aaaaagattc tgctccggca atgcctgata tgggcggaat gggtggaatg ggcggcatga
     8221 tgtagtttct gtcataatgt caatttgcct atttgtcaca agataatcta tcgtaaacac
     8281 ctgaaataaa aagacatctc gtctggtata aaaattgcat ttatgggggt cgttttgttt
     8341 ttgatgaggt gatcatttta tgaaaagtag gacaattcta aatgccgggt tgtttatttc
     8401 gataactttt gcgatagctt ccacggtggg ttttcttctg agtttcattg tgaaggatga
     8461 acaggttttt gtgcccattg gaaaaagcta ttacaggaac ggacttgaag aggatacgga
     8521 agcaaaaaag gagagtttct atttcggcag caatgttgaa aaggtttctt catccaatat
     8581 tttcgacgcc gcaatgagga acatcactgt tgctgacaat aaagtgggag aaacaacagg
     8641 tgacggtgcc gttcctgagg cgagtgcaga tgacatatat ttttattttg aaaatcctga
     8701 aaaatgccct ctgatgtcct cgctgctgtt gagcggaacg attgtcgcaa aggagttcga
     8761 tcagtcaatt gcgatactcc gcgaaggaag cagcagggaa gaaggtgcca ttattgccag
     8821 agtgggatca gaagtcaccc ccggagtgaa acttgctctt gtctggagaa attttgtggt
     8881 tctgacaact tcttccggtg taaagtgtat cggtgaaggt gtcggcggtg atgcaaaaca
     8941 aagtgttcct gatcagaggt ttgcccagac aggagagccg cctgttgccg gaaatgacgg
     9001 cattgaagta agaaaggtcg gtgaaaacca gtatgttata agaagacagg atcttcagaa
     9061 agttacaggc aatctcaatt cacttgcaag acaggccagg atcgttcctt caaaaaagga
     9121 taacggtttc aaggtgttct ccataacaaa agacagtctt tacagtaaga taggtattca
     9181 gaacggagat gtgatcaagt caataaacgg catagaactt tcttcgccgg acaaagctct
     9241 tgaggcatac tcacggcttc agagtgcatc aaaactttcg cttgatattg taagacgcgg
     9301 tcagaatgaa acaatggaat atacaataga ataggagaaa aacatgactt ctaagaaaat
     9361 tctctcactg atgcttctgc tttttctggc tttttacgga tatgcggctg acggagacga
     9421 cgatgtaaaa gttcctgtga atttaaataa ccggctcaaa gggataaatc agaatttcag
     9481 tccgaagttc tctcccaagc ttgattctgc taaaaagatt aagaaaaaac agaaagatgg
     9541 taaagaagaa aaagaagaaa acagtgaaaa cattcagaat ccttctgaaa agccgggtgt
     9601 gaagactgtg acaccgcctg ctccgaaaac aatacttgat aagaagagcg aagatgatcc
     9661 tgacgcctca aagctgagcg atgcagtaaa gatcgaggtt gcagaaacaa aaccggtcga
     9721 tcccaacatg aagatcaagc ttgatttcaa ggaagaggac ctgataaaca taataaagct
     9781 cttttccgaa ctgatgcaga tgaatttcat aattcctgag aatcttggaa aagcgaaagt
     9841 caacatgatg gctccgcaga aagttacggt gagagaggca tacagaactt ttctcacact
     9901 tcttgcggta aacgatttca gtgtttcaga ggacgggaaa ttcacaataa taacaaggga
     9961 aaagaatatt ccggaaatga aaattccatt ttataaaggg acagatgttc ccgatctttt
    10021 caaaatggtc gcaacgatca tgaaatttaa ctatgtaacg gccgccgata tggataaggt
    10081 tttgaagctt ttcagagata agggtgcgac atcacttgtt tttgatgaca agacgctcat
    10141 tgttgtcgac tatgcggcaa acatcagaaa aataatgatg ctcgttaagg aacttgatca
    10201 gccctctgag tctgattcgg caaagctcta ttttattaaa ctgacacatg ttcttgccgc
    10261 tgacgcaaga aagatactgg aagacatatt caaggatttt tcaaaaaaag ggacaaaaaa
    10321 gaaaagtgcc ggctcggaca attcacccaa tcttgcaggt gttgcaaatc cacagcgtct
    10381 tccaggttct tcaaggaatc ctgccgatcc tccatcagga gatgaagaga gtctttcagg
    10441 tgaaaacatg tatcttcata ttgttgcaga cgaaagaagt cagcagctta ttgttctatg
    10501 tacccagaca acatataacc tgatcattca gatagttcag aatattgaca gagaagtgga
    10561 aggtgaaggc gagatacatg ttgtgaaact tcagaatgca aaagcggaag atctcgtaaa
    10621 aacgcttaat cagctttcaa agaacaaaaa atcaaccggt tcaaaaacaa cagcaaaagg
    10681 gacagatgtc tttgagggcg aggttcagat atcttcaaac gaatcaacca attcgcttgt
    10741 cgttgtgtca tccatgcagg atttcagaaa tctgaaaaag gtcatagaaa agctggatat
    10801 aagaagaaaa caggtttttg tcgaagcggc gatccttgaa gtgaaaattg acgactcact
    10861 cgaatacgga aatgcctata ctgcaggcgg atttgaagtg aatgttgccg gcgagaagat
    10921 ccctcttttc ttcgggaagg cgctcgattc aaaaatgtct cctggaactg ttgcaggcat
    10981 ggtggctccg gcaataagcg gcactgaagg tattcccggt ctgggacttg caggcggaat
    11041 tccttcgata ggtgttattc ttaatgctgc acagaacgat tcatctgtaa atgtaatgtc
    11101 aactccgcat cttcttacaa cagacaacga agaagctgaa atatctgtag gtgaaacgat
    11161 acctttccct tccggaaaca tcatcagcgg aaccgcagga agtactgtta catatacaag
    11221 ggaagatgtc gctttgaaac tgaaaattaa gccgcagata aatgaaagcg gatacatgac
    11281 tcttgaagta aatcaggaag taacagagct tggtgcacca tcacagtatg gattcactac
    11341 gacaaaaaga caggccaaga ccaaaataaa tgcagaaaat gaacagacaa tagttatcgg
    11401 cgggttgatg aaagattctg taacggaagc cgaaaacaaa gttcccatac ttggtgacat
    11461 tccagtgctc ggagtatttt ttaaatataa gagaaccgta aaagccaaag taaatctgct
    11521 cataattctt actcctcata ttgttgaaag caaagaggat tttgcacgga tacttaaaag
    11581 aaaaatggaa gaaagagatg aatttgccag aaaattctat ggtgaccatg tttcatttga
    11641 agacaacatt tatcttgata agaaaagagg agctcttctt acggttgtga aggaagttga
    11701 caatacaaac cgcaaagagg atgaggaact gctcagaata aaatcaaaga aggatgagaa
    11761 gtcgatcatg gtcactcccg atggagagga ggagatcatc tctgttcctg aagggtctct
    11821 tgacagggag gacgatctcg ttcctgaagc tgtacctgag tttgacggag aatagcatgg
    11881 caaggaagaa gataggagaa atacttattg aaaaggggca tcttgatgct tctcagctaa
    11941 gccgcgcact tgaactcaag agtcagagtg acaagaaaat aggtcagata ctggtttccg
    12001 aagggataat tacccctgaa caggtggctg aggcctttgc cgagcagaca ggcatccctt
    12061 tgatacccga agattttttt aaaaaggtcg atccttcgct cctttctgat tttcccatcg
    12121 gtcttgcaag aaagttcaaa gcgatgcctg tttttgccga tacagagctt catgttgctg
    12181 tgacggatac ggacccgctc ctttttaatc agctcttcat gctctacaga aaaaatgtta
    12241 agccatacat ctgctctact tcaaagatca atgaccttat aaatcacgca tattcaaaga
    12301 tagacagcaa tgaagagtcg ctgaacgccg aagaatttgc cgatatgaac gatgattcag
    12361 atgttgcgat ccccgacctc atagattcca atgacgaagc ccctatcata aggtttgtga
    12421 acaacacgat agccaaggcg gtaaaggata aggcaagtga cattcacttt gaatcgtatg
    12481 aagatgaggt aatagtccgt ttgagaaaag acggaaaact ttatacagtt cagagggttc
    12541 cgaaagctgc ccaggcaggg cttataacaa gaataaagat aatgagccgg cttaacattt
    12601 ctgaaaaacg gcttcctcag gatggaaaca tcaaggtcaa gatagcggga aatgatgtcg
    12661 atttccgagt ttcgacactt ccaagcattc acggtgagtc agtggttctc cgtattcttg
    12721 ataaaaggtc tcagcagttg tcgcttgatg aaagcggatt tacaaaaaga gatcttgaaa
    12781 ggatgagaaa gataatcaga atgaaacatg gaatttttct tgtcacaggc ccgaccggaa
    12841 gcggtaagac gactactctt tattcggcac ttaccgaaat aaattcaccc gatgtaaaga
    12901 taataacaga agaggacccg gttgaatatc agctcaaagg tgtcaaccag attcacataa
    12961 actcaaaaat aggacttaca tttgcaagcg gattgagatc gattctaaga caggaccccg
    13021 acataataat ggttggagag atcagagaca gggaaaccgc tgatatcgcg ataaacgcat
    13081 cattaaccgg tcaccttgtt ttttcgacac ttcacacaaa cgatgctccc acggcattta
    13141 cccgtctgat agatatgggc atagagccgt tccttatttc atcaaccgtt cttggtatac
    13201 ttgctcagcg gcttgtaaga aagctctgtc ctgtgtgcaa ggaggcatac tatcctgagc
    13261 cggcaatcct tgaggaactg ggacttgatc cggctgaata tgcgaaccat cctttttata
    13321 aagctgtcgg ttgtgacgaa tgtgctcaca ccggatattc cggcagaaca ggtattttcg
    13381 aacttgtaat aatggatgac gagctcagaa gggctgtcgt aaaccgtctt gacgcttcgg
    13441 acatcagaaa gatcgcctct gctaacggca tgcttaacct cagagaggac ggagcgatga
    13501 aagctataaa agggataaca tcacctgatg aagttctgtt aaggacacag gaggataact
    13561 gatgcctctt tttgcttaca aaataataga ttcaagcgga aaggaaaaaa aggggaacat
    13621 agaatgtccc aacagaaatg ctgttaaatc caaatttctt tctgaaggat attatattac
    13681 tgaaattcgt gaacagtcat ccgctcagag tttcagtttt gattcactga aaagcatatt
    13741 gtctttcgga agaaaaaagg tgcctctgga tgaagttgct tcaatgacta gacttcttgc
    13801 cacccttcag aaagctaaga tccctcttgt ggaatcaata gatgccgttg cccagcagca
    13861 ggaaaatcag atgctgaaag aggcactcat aataattaag ggaaaaatga acgaaggtta
    13921 cagtttcagt agaagcgcaa gggattttcc caatatattc gatgatttgt attgcaatat
    13981 gcttgccgcg ggagaagaga gcggtgcaac ggacagggtt cttctccgtc ttgcaggttt
    14041 tatggaatca caggtcgatc ttagaaacaa agttaagagc gcaatgacat atcctgtaat
    14101 actgatgttc gtcgccatta ttgttgtggg cgtgcttttt accgttgtaa tccccaagct
    14161 ggctaaaatg tttgaagatg tcaacatgtc acttcctctg cagactgaaa ttcttatctg
    14221 gctttcgggg tttataggaa gctggtggtg gctgatagca attgtaatca taacggcgtt
    14281 tgtccttttc aggagacata tcgcaactga aaaaggggct ttctggtgga gcggatttct
    14341 aattaatatg cctcttttcg gcagagtgaa caggatggtt gcggtcagca gatttgcaaa
    14401 gaccctctct actcttcttc attccggtgt gccgatcctt aatgcaatgg acatagtcaa
    14461 gaagatcatt gacaacaaag ttcttgaaaa agctgttgcg actgcaagag agaacataaa
    14521 ggaaggtgaa tcgatagcag tccctcttaa aagaagcgga gagttccccc cgatagtaat
    14581 tcagatgatc tcgataggag agcagagcgg tgatctggaa gagatgctgg acacccttgc
    14641 agacagctat gaaaaagagg taagatacac gatggatcgt ctgacttcaa tgcttgagcc
    14701 gataatgata gttgctctgt cagttgtaat tggcttcatt gtatttgcgg tcgtaatgcc
    14761 gatacttaag ataaacgatg ctgtaggata atatttcgga ggtaaaaatg tttaagaaat
    14821 tttttaaagc ccttcttgaa gaacgggccg gcgtggcagt taagggcttt tctcttcttg
    14881 agatcatggt cgtcatcacg ataatgacga tgatcatggg tgctgtaggg gtcggagtaa
    14941 tgtcttatct tgacaagtca aagatcaagc aggcgagaat agatattgcg actatatcaa
    15001 acgccctcga cctttataag actgagtttg gcaaatatcc tgacagtgaa gacggcctga
    15061 gccggcttgt tgaagagaaa atactgaaag agaaaaaagc ccccagggat ccgtggggca
    15121 acgattttat atacatctat cccggaagca acaacgaaga cggttttgat ctttactctt
    15181 tcggtcccga tggaaaggaa ggggggaacg atgacattgt gaactgggat acagaataga
    15241 ggtcttatga aaaaaaattc cggattttcc atgatagaaa tgcttgccgt tgtggctctg
    15301 gtcatagtca tggcaggtat ttctgttgca ggcgtaggaa gaatcggaag gtataaggca
    15361 tctgcagatc ttggcacatt caattccttt ttaaggttca attttatgcg ttctgtcaga
    15421 aacggtgaat atataaggat cgttattgat atggaaacgg gctcatactg gtctgaaaag
    15481 agcgaaacac ctttttttct ttcaacaggg gaagcgacag aaaagaaaaa agaggaaaat
    15541 gatgaaatgg tagagagaat ggaaaacggt atggggacag atccattcaa agaaggcggg
    15601 tcggcgctcg gaatggagag cataatgcag aaggcacggt tgctgtcaag cgaagatctt
    15661 gataacgatg attatttcaa ctatgagaat ttcatccccg acagaagatc tctcaaaaag
    15721 atactgcaac ctgagtttga gaccgtttcg aatcagaaaa agatctcaga cggacttgtc
    15781 gtgactggct tttttgcata ccacacacct gaaatcgtta ctagagatct cattaccgat
    15841 aaggaaatgg aaaaaaatgt tcatatatac atttttcctg aaggccgtat tgaacccttt
    15901 tatttttctc ttggagagga aagtgatgac ggtgaaacca gttattctta cattacatcc
    15961 gacatgttca tgaacacaaa gatccgtccg ggaagctttg aagaggatat aaaggatatg
    16021 aaagatcttt ttgaagataa agatgaggaa gggagatcat gacagtgtca aagggttttt
    16081 cactcatcga agttcttgcc gccacaacga tccttgcagg ggtgctcttt tctctttcaa
    16141 gaattatatc aggaagttat atatacactt caaagataag gaacatatat ctggggacag
    16201 aacttgcaag acttaaactg cacgatattc aggaaaagat aaaggaggac ggtcttcctg
    16261 aaactgaaat ggaagaggaa ggtgattttg atcagaggga atatagtgaa ttcaagtgga
    16321 aatactcaat aaagcgtgtt ttcatcccgc ttcctgatat ttcgagttca gctgacgacg
    16381 gaacccagac tcaggcggaa gaggcggcag gaatgctctc tctggcaaaa gggaacatag
    16441 aggatttctt taaggaaagg ataagaaaac tttccctcac tgttttctgg ggacgaggtg
    16501 atcgtgattc agagaaagtc gtttttacaa tttttcttac aactgacggt gcagtaaaga
    16561 gtttccagca gtatcagggc ggaggcggtg ttccgggtca ggatccttct cagggaagag
    16621 cgagaaaatc agatcccggt tacaacaggt tcaaaacccc tttaaacaat cccccggctg
    16681 gtcttcccgg aacggaacag aggtgaaaat gagcagaaag ggtttttcac tcattgaagt
    16741 tcttgtggcg atgtcaatta cagcaattct tgcgtcatcg gtctattttt cattttcaaa
    16801 tgtaatagca ggcagatcaa agatcaagga gataaccgaa aaagagagaa aaacatattt
    16861 cacgctcgaa ctgataagga atgatatcaa gaacgcattt ctcactaaca acaaaggtgt
    16921 gcctgaagag acacacaaaa cgatctttaa aagtgaagag gacgaccctg tcagtcattt
    16981 gacattcaca acgctcaacc atgttaaaat ggccgcaggt gtcaaacagt gtgaccagac
    17041 tgagatcgaa tactacgggg agaatgtgga aggggagaat gttcttttca gaagagaaag
    17101 tctgtggata gatgaatttc ctgaaaaggg gggcaatgta tatcctgtaa tgagcggttt
    17161 tgaaagactt tcatttcagt tctggaagga atcaagcaaa gaatgggtcg atcagtggaa
    17221 cacagaaagt gcagacaatc ttaacgctct tcctccgaaa gttaagataa caatgctcgt
    17281 taaagaaggt gaatcgggtg aagaggatta tattatagaa acagtggttg agataaaaat
    17341 gctcagacct ttaagctttt aggtgacaat gaagaagttt ttttccaaca tgttcaagtc
    17401 atttaacagg ccgaaaagag gtgtcgccct tcttatcgta atcgttatga tatcgatact
    17461 tcttccgata gttaccgaca tgaattacga agcaaagact gagtttgacc ttgccattaa
    17521 ttataagaga aaagctgaag cacagcagct tgccgcaagc gctgttaatt ttgcagttgt
    17581 cattttcgac cttcaaaaac agatagaggg gatcctcaag cagttcaaca tgggacagca
    17641 gattgaaata tgggatataa ttccttttga caccgctctc ttaaggggtt ttgtaacggc
    17701 aggacctttt gtagaacttg aggatgtagt gacaaaaaag gatgttgaaa accccaccgg
    17761 ctcagcactg aacgatcagg aaggtgattt ttcacatgct gaaacaggag atccgatatt
    17821 tcagttcccc ggtgatttca aggtggaatt tacaggtgag gatgacaaga taaacctcaa
    17881 ccatctcgcc tacggtacaa gatcagcagt cataaaaatg cttgaagcac ttatcgagcc
    17941 ggagttttat gattttatat ttcttgaaaa cacttcaagg cgtgaatatg tggaccggct
    18001 ggagcttatt cagaatctcg ttgactgggt cgatccggga gatgacaaat tcaccgacgg
    18061 gacaaagttt ctctcagggg gagacgagca gtccgactat gccgattttc tgcccgaata
    18121 caaagtgaag aatgcaaagt ttgacacaat tcaggaagtt atgatggtct atggtgttga
    18181 tgatcttata tacaagatcc ttgaaccgca cataacaata tattcgacag gaaagatcaa
    18241 cataaacaag gccagtccgg aaatgatcga aggcattatc agggcatatg cagtggacaa
    18301 ggcgatgaca gttttttaca gtgaagatgc gatgagagaa cttctgggga agatacttgc
    18361 aaaaagggcc cgtgacggat tcgccaagac agttgatttc gttcaggcct gcaaagatga
    18421 aggaatagac atggaaagcg atgtggagaa gatcatagat gtgtcgggaa gggtttacag
    18481 aataaaggca acaggcataa aagaaggcgt cgagagctgg gttgagatgg ttgttgacag
    18541 ggaaggcaat atttattact accgtgaagg gtaaagtcag aggtgaaata tgaaatttgc
    18601 ttcttgcaga gttatagccg gcaagttact cattaatgtt ttttccaggg aatacaacaa
    18661 gactgtcctg gagagcagaa cagaatcttc tgttgaaaac tgtgcagaaa tatttaaaga
    18721 ggttgattat atcgatcttg ttataccggg aaaggaagtt cttttctttt caaggaatta
    18781 tccgcctgtt aaatcttcat acatcagaaa aataatcgtt caggatatcg aagttgaaac
    18841 accgtttcgg gaaaatgatc ttgttataga tatgaaaagt ttttccggaa acggcgaaac
    18901 aagcgttttc tgtgcaaaaa aagaagcagt gaaaaacatt ctggacctat ttgacgctgt
    18961 gacaagagat aagatcagaa gcattgttcc ggaagagata ctccttttca aactgccgga
    19021 aaagatagac agagctcttt tcatcggtga tgattactca atgctcgttt taaggaacgg
    19081 acagataata atgaacggag ggatcaacga tcttaagggt gatctgagga catgtttcag
    19141 cggcgaggat cctgacagcg aacttaacgc atggctttca gttgcaggtg acataagtgt
    19201 tcctgaaggg ctgagtgata ttgaaatgct gataagaaaa agagtcattg cttttatgga
    19261 aagagttttc ggttttttcg gaccgttcac aaattcttcc ggtatgacgg gagtcttcat
    19321 taatgacgat cttgtcaacg gaatgggaag acttattgag cagatcgatc atccggtctt
    19381 tccgggaagt cagtcaattg tgtatgaagc gtacgaatca ctcgagatca tacctgagat
    19441 agtcgataac gacagctcag taagttttgc aacgggtgag tttgcatata aaggtggatt
    19501 ttcgttcctg aaaaagagaa ttgtgactgc ggttgttctc tattttacag ctctgataat
    19561 gctggttgcc ggaatgcaga tgagaataag ctatcttgat gagaggatcg aaaaaggaga
    19621 ggagagaacg aagacgctga tgaaagatgt aatagggaag gaaatgccgt cattaaggca
    19681 ggcgctttcc attatgaaca aaacaataaa aggtgaaacc gcacttgcag ataaaaaggc
    19741 ggtatatcca tacagcgcac tttatatcat ggaggtgacc tttccttatt ttacttttga
    19801 gaacagcact atcgaagttt ctgaacttgc aataaaggaa gaagggaaga tcagagtgac
    19861 agggacaagc agcactcttg aggatattaa caaattcacc ggatttcttg aacaggatcc
    19921 tttgatcgat gagataaaca gaggacagat aaacacaaga aatgataaaa gttccttcag
    19981 tataagtttc agctatgccg gtcctaaaaa agatgacacc aagggcaaaa aaggtaagaa
    20041 aaaggattct gaaaaggatc ccggggagga aatatgaaag aaaagataat ggaaatgctc
    20101 aacaacctga gcgaaaggga aagaacggtc cttacttttc tcataacctt catgatggtg
    20161 tttatgttcg gagggatctg gttccttatg gatatgactg taagcaacag aaaagatact
    20221 atttctgagc aggagacgat gatacagaaa atggtttcga tgaaaactct ttatcagaaa
    20281 gctcagatca ggaacgaagc aatgaagagc agtataagca caagcagcgt aaatcttaat
    20341 tccgatattt caacggtcag agagagtacg ggaattggta taagcacatt aaaagagctc
    20401 aaaccccgga agaaaggtga tataagcata gagagaatag aactcggatt cagagatgtt
    20461 tctcttgagc agacaatggc atttctttac ggtattgaga acaggggcag atatgttttc
    20521 attgattcaa taaccataaa aaagagattc agcatgcaga attatgatgt ttctgttgtt
    20581 gttgccgctc tcaaaaagga ggtgggtgtt gaataaccgg ttatttggaa aaataaagga
    20641 gatccttgct tcggggcgtg ccggatctct tctgaaacag acacttttta ttaacattgc
    20701 tttttttgcc gggatttggc tcggagtcaa gatatttttt cctgatgatt tcatcctttc
    20761 aaaaataaat aaggagcttt ttgttaaaga tatgggactt accgcagatg atgtcggaat
    20821 atcgatcctt ggcaacattt catttacaga cggaactttg accgaaaaag gtgtgaaagt
    20881 cgtcactttt cataaactta aattttcacc ctcgatcttc agcattctcc aaggcaacat
    20941 atcaggtgaa ttttcgataa atgacatcaa taaccagggc ggagatctga cggtatcttt
    21001 tgaaacttct caggagccct gtta
//
LOCUS       DAOOYB010000013        20697 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_2424_length_20697_cov_9.238010, whole genome shotgun sequence.
ACCESSION   DAOOYB010000013 DAOOYB010000000
VERSION     DAOOYB010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 20697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20697
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2424_length_20697_cov_9.238010"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            156..2843
                     /locus_tag="PL056_01550"
     CDS             156..2843
                     /locus_tag="PL056_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HPM45730.1"
                     /translation="MTESEWITRKTRIDSKLNSLSQPWKIIPYSENLDTSKLTNHAVE
                     EFPTANGPADYAFFVNGRFLGILEAKKVSLSPQNVLEQAKRYSMGAEKSIGQWRQFKV
                     PFIYSSNGTQIWFADLRDDQYYSRELMNFHTPSAMFEMFERKSGKFRDYFENVPNDIE
                     RLRPYQKLAIANVEKGIIENKRKMMLAMATGTGKTFTTVSMIYRMIKSGYARRILFLV
                     DRRALAAQASQAFSAFQTPSGNKFNQEYEVFSQRFRKEDFEEGEKFDIGVLPNEYLTN
                     PNTAHTFVYVATIQRMAINLFGHEGSFGQSASDAEADDDAGKIDIPIHAFDVIIADEC
                     HRGYTSKDTNIWRSVLNHFDALKIGLTATPASHTVAYFGNPVYRYTVNEAVTEGYLVD
                     YQAVKIKSNVRINGIFLSEGEKVALKNKQTGIETIDQLEDEREFNSSDVERKITSPDS
                     NRKIIEEIAKYAKEHEEKTGRFPKMLIFAVNDIAHISHADQLVNLAREIFGRGDEFVQ
                     KITGSPTVDRPLQKIRMFRNRPEPGIVVTVDMLSTGVDIPALEYIVFLRPVKSRILWT
                     QMLGRGTRKCVDINKEFFTIFDCFDGTMIEYFKDSTDFVIEVTESKDAVDIKQIVENI
                     WNNVERGYNTNRLIKRLRRVADTMSAKARDDFSKFIPEGAVTPFTDNLKDNLRSSFNS
                     TMDILRNKEFQELMENYDRARNPFYVSYSTVDTVSSDLMFKYGEKSLQPADYLEAFSK
                     FINENEEKMEALKVLLNNPRKWNSAALKEIRNEMKRNSFPEEKVQQAHRLTGHKALAD
                     IISMIKNAKDHKNPLLTASERVETAITKLMKKSTFNTEQTEWLNYIKEHLIINLAIDK
                     DNFDLVPVLERHGGLAKAKKVFGNQFEEIIEMINEAVAA"
     gene            2871..3704
                     /locus_tag="PL056_01555"
     CDS             2871..3704
                     /locus_tag="PL056_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45731.1"
                     /translation="MKKITFHGEITNELGVIMAFSKIHEELGFEKIVCSSPIGFDIGS
                     IQYNGNDVTVEFEHKSSNFLTHGHPSKMEKGRKYVVICWEDDCGLMQKIKKDYKKDIF
                     EVIEIRKYVKIDNENSPVDNKDEEPICGILSYNPKYAGDVDFGNWAFVNCYRVNTSEK
                     SRKFKKNEIPKGSKFLFYRDGFIIGGFTVVRYEIIEEPKTKREMEIYKKLTDYPITHY
                     SLTENEISENYLRGHIFYTDFFDMRDYKKNLSKYKINKQMGRQGYITIDRETYYRILG
                     E"
     gene            3784..5010
                     /locus_tag="PL056_01560"
     CDS             3784..5010
                     /locus_tag="PL056_01560"
                     /inference="COORDINATES: protein motif:HMM:NF022633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2971 domain-containing protein"
                     /protein_id="HPM45732.1"
                     /translation="MGFGWFSEKKSNYDRKDFRSLNYKGRWQRKFIDEFVEKLFVFEN
                     RDGVYSEMSICKEEHMPEFLYKFYRPSSNSIVNLENNLLHLSSPEEFNDPFDSYVCID
                     EDKFAKYRLLKTLKEKKHISKDETPDTLSEKEYWEIFYSTTSDNHNYKRKNFDSLIWE
                     IKDKKSKEFQKVLTSIEVDSWRECKEKIKHLRKSCYKITCFSNFSDEFKLGINTTMWS
                     HYADNHKGFCVKYPLKLNELKHKDLILCGLYPVIYTARIPKVSPRELIKSKFLNDKPQ
                     TNKQIQKTVLKSFITKSRFWNYEKEWRLIISNQDDYIFSDGTIPFVKPESIYMGCRIE
                     NNLKKHLVKYADKNGINIYQAYKSEEKFELIFRKVNMESIKRDEYDQKCHDYFRKEDE
                     REKGYLIRKLNEFEKE"
     gene            5018..5617
                     /locus_tag="PL056_01565"
     CDS             5018..5617
                     /locus_tag="PL056_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012621943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45733.1"
                     /translation="MTFNPEIHHRRSIRLRDYDYSQNGAYFVTVVAKNREDLFGEIEN
                     GEMILNDTGKMVLHCWEQIPEHFPDVEIDECCVMPNHFHGIFLINCNRRGVGCKGERF
                     FAPTDSGTSKTVGSIVRGFKIGVTKWARKNLEIIDVWQRNYYEHVIRNESDLEKIRQY
                     IFDNPANWENDENNVRAKNFSPKETITDHVRAKDFSPLR"
     gene            5633..7024
                     /locus_tag="PL056_01570"
     CDS             5633..7024
                     /locus_tag="PL056_01570"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013451965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HPM45734.1"
                     /translation="MSDIVNKLWGMCHTLRHDGVDYGDYIEQLTYLLFMKMADEKSVN
                     LPERCDWESLKSKSGTELTDHYLMVLQKLRDAPGLLGDIFAQSMPKFNNPVSLKKIIT
                     MIDAEDWSSLGVDVKAAAFEGLLEKAASEGKKGAGQFFTPRPLIQSIVKIMQPDPLKQ
                     KGMKICDPACGTGGFLVCSYEWLIEKTKGAIPVDDVPAIKGTTYYGQDLGARPRRLAL
                     MNLFLHGLKPVIYLGDTIYEPDKGERYDVILTNPPFGNKGAGQAPMRDDFTISTSNKQ
                     LNFVQHVLTVLKPGGRAAIVLPDNCLFEDKAGEVFEIIMKDCNVHTILRLPRGTFIPY
                     ANAQANVIFLQKGRPTEKVWIYDCRSNIPGCTKKDRPLTGEMFTDFEKCYGEDPNGNS
                     KRTDQGETGRFRFFNIDEIKKRDYKLDLKWLKDDTLDDPNDLPEPADLITEAVTELEA
                     IMKELNEINELLS"
     gene            7149..8483
                     /locus_tag="PL056_01575"
     CDS             7149..8483
                     /locus_tag="PL056_01575"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HPM45735.1"
                     /translation="MNSNIPKHWVETTLPNICKIETGKWDANHAEENGQYRFYTCAYE
                     FQHCNTKRFSGDCIILPGNGANVGEVFFYSGDFDAYQRTYVLSDFKIDPRYSFFHMLY
                     KWREINKDKQFGSATNFIRIGNFNDYVFEIPPLNEQKRIVEKLDTILPKVKNAKARLE
                     NIPKILKKFRQSVLADACSGKLTEEWREGKELPEWEEVTLKAIADIKGGITKDTGKQK
                     KADIEVPYLRVANVQRGHLNLSEIKTIKIPKEKLEQYLLKHGDILLNEGGDPDKLGRG
                     WIWESQIETCSHQNHVFRARLFDSNNQPKYVSWYANTVGADFFFQSGKQTTGLASINK
                     TMISNLLINLPPIEEQHEIVRRVEKLFAIANSLEEKYKTAIARVEKIEQSVLAKAFRG
                     ELVAQDPNDEPAENLLKRILDVRAKDPIFAKLRRTGLSPLRRKGKTGKTKSK"
     gene            complement(8800..9606)
                     /locus_tag="PL056_01580"
     CDS             complement(8800..9606)
                     /locus_tag="PL056_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45736.1"
                     /translation="MSIRRTLSLVCAVLFLSVFYSCSAKDDSEEVTDKTQETDNNNDL
                     SDDLSDEALAESEDTESEDAESEDPEPDKTPDETPDETPDDTAPSGEGAYRQCDPAMA
                     GSCPEGMNCVKGYDNAKYGQCLKPCSENTDCPPPPETTQQVACHNTEKVCLILCGSYE
                     GECPEWLECFGQEMCLPPSGITPTKGPGEKCNGKDECIGDSDCIEGSSGIAYCYPLCD
                     PNVADDYANKAPGFQAQCTNAGSFSFCMFNCQGVPCPGELQCVAGVFCQG"
     gene            9725..13006
                     /locus_tag="PL056_01585"
     CDS             9725..13006
                     /locus_tag="PL056_01585"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HPM45737.1"
                     /translation="MKKITDIIKRSLKNEPTRTVSIDPDSIIASLAESTILAEKKDDW
                     KYRIEDEIGRGGIGRVLIAFDEKVGREIALKELIKEDNPGSVTVAPLDKMKADEIRFL
                     REAKVTGQLEHPGIVPVYEIGRKPDGNHYYTMRLVRGVTLSQAIYDAGTLQKRLRLLP
                     HFRDICNAIAYSHSRGVIHRDIKPENIMIGEFGETVVLDWGLAKVKGEADQAEGELRE
                     ELNLLKVDNIGKTLKGKAIGTPAYMSPEQANGEISEIDERSDIYSLGAVLYEILTGHA
                     PFKGRDVKETLEMVKLNTPVEITIFEPEAPPDLCAVVSKTLTREKESRYQSAMDVARE
                     IENFMSGGRIKAYEYSSWELFKRFSAKNKLLTGLLATLAVVMIFGSVVIFYAYRESVE
                     NERSAHLNLSLGYLEYAERLIKEKRYLNAKIFSAASLYHNPYNPKSPWSFPEFFMTEN
                     AIESSNQMLSVQSVLYLSTVHQNDSFKEDLAQMDFDPRVLVISPDGSLAAVAGKTRRI
                     MVYSVSTGKLKYTLNGHSDEIAALEFSPDGKYIASGSWDRKLNIWDAREGILIKTIES
                     STGEIYALSFSNDSRFIAFGGTDRNLWISDISDFQLKARKIMELENTVRAVNWSPDGK
                     ILASDSSGNIYLIENGKLKSVFKRHSEPVISLKFLKDSNYFISTGYDKRFLVWNINAS
                     APLYIYEHWDAFFDLDISSDGTFAAAASRDGTVKLIDLRNRQIEDLRGHEGAVYSVSF
                     MPVGNALMSVGEDHLLKVWKTDVKRLVQTYRGHATYIPSLKYSPDGRFLASSSWDNTV
                     KLWDVNREMLISTFGTPGLVSYSIDFSPDGQFVVTGDSDGFIRMWNPVNGKLLSVMKG
                     HSDQISSLAFSSDGRYLVSGSKDRTANLYDMSARKIVKIFKHINPVHAVSFSEKGDVM
                     ASAGRNGEINFWRIPEGDLIFSSQQGKTILSVAFSPDGSQAVSAGEDGTAIKWDVKNK
                     KMDKTIYGHTGAINGVVFSNDGSLFATVGREVIIHNGKTSEKLLAFDLQYNGYSIAFE
                     KDGTHFAVSDGALIKRYPVILEMWKEDPVKMLEEAEHKAGKKLEGFKLSPISEKITVT
                     EETSE"
     gene            complement(13008..13835)
                     /locus_tag="PL056_01590"
     CDS             complement(13008..13835)
                     /locus_tag="PL056_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45738.1"
                     /translation="MKILWCIIFLVAFLGFNHTTLYGTESGTNLKTMKTSGNTEWESA
                     VKYAQKYKKTYKRENAGGYSLIAIGAASILTGCVFLGVTRGGGIILAAPFWSIGLTLI
                     SSGLILRSSADYKLLTSEEFNKISILGDHPDIDAYQFYETSGIKRSVKKNASYKFRTN
                     GIILTSISIPLFALAIGGFVGSAQIDKENREESDDQTMDEAMGSMGAAFASVGFFILG
                     ISSIVPAVLILSGGITLLSISSSWKRLGAEPALTLNSISPIIDPVSRTYGLSAGFSF"
     gene            complement(13846..14886)
                     /locus_tag="PL056_01595"
     CDS             complement(13846..14886)
                     /locus_tag="PL056_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45739.1"
                     /translation="MKNFKLSVLFLLIIIFTHQVYAEDCYEYGESYSKLVFRADGYIK
                     KGKTKQAARLLKEHLECWNNDKNVYVKLAEIYEYEQKYYLAGLAYKKAGMTQKYNEVT
                     LKAQNSRHHGFQQYAISDSARYSKRYEGFKAFSIVFLSLGPIATAAGISLYVVDRLSK
                     REYSMGLQYGLTLGGLSFIAAGTLMSSGAVNAYNRAYVYRKISLEYSGDPGTTPEEYF
                     ENSKHQRNFNIATADNYQKHGLALMGISIPLIGIAVYGFFDSYNRIKESNKDHGNWDP
                     TGLDTFFAHFLQIFSLAPGIATFVGGTILLAKAARHEKAGKTHYLLTLDSVAPIIDPV
                     SRTYGLSAGFSF"
     gene            complement(14959..16089)
                     /locus_tag="PL056_01600"
     CDS             complement(14959..16089)
                     /locus_tag="PL056_01600"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPM45740.1"
                     /translation="MTNILSFLTFLTVVISISILMIRYFIHSMMGGSRRMIKYRRELM
                     LLYIPIFLSFFLFSNRSLQGSLSLSIFSCVLYAFFTFMFVFFLYGLFVELLILIRKLF
                     KNIYSKKLPLHKKRLVFIVLFLISFHTVATGFFLAEDIQVVTLKIKNPKIEKYTRIVQ
                     VTDIHFSATTGLPFAQKIASIITELNPDILISTGDYLDHGMMEPERIAEAMNSIPAPL
                     GKFAITGNHEFINDFNNSKNFLEKNGFKLIDNQIIDVWRNITISAVADKTGKRFGMSA
                     PNDLDILKKAHHSRFNIFLKHQPKIEKGATDRFDLMLSGHTHAGQIFPFGIFVRMAFK
                     YCSGNYKFNSGSILHVSRGTGTWGPPVRFGSMPEITLIELIP"
     gene            complement(16167..16841)
                     /locus_tag="PL056_01605"
     CDS             complement(16167..16841)
                     /locus_tag="PL056_01605"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HPM45741.1"
                     /translation="MFGKKKNRTAVVIGLGEFGLHLALSLSKCGCDCIVADIDAGKID
                     SIKNFVMKAIIADASQKNALYQIVPADADFVIIALGDIESSITAALYLKDMKIENVYV
                     KAISEEHERILKMMEVKNIIFPEKDMGQRFATKLMSNNLLDFLPLSEDYSVAELAPLS
                     SMANKSLKEIDFRKEHNLSIIAIKELVPPNMVISPSAGFKIKISDILVVLGKKDAIEA
                     YNQKTH"
     gene            complement(16853..18190)
                     /locus_tag="PL056_01610"
     CDS             complement(16853..18190)
                     /locus_tag="PL056_01610"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HPM45742.1"
                     /translation="MNNRKITGSHILVFSFLMLIFSGMLLLKLPFASHTGTTWVDALF
                     TATSAVCVTGLIVVDTPVHFTMFGQIVILVLIQLGGIGIMTFAASFIFAFNRKLPLSS
                     RMMMEESFMQGAADIKLSELLRFIIKYTFIFEISGAFLFFMTLDENNLFKRLYFSVFH
                     SVSAFCNAGFSTYSDSLMRYRGNISVNIITMILIIAGGIGFLVTYEIKSKLKGLFFNR
                     RKKTRLRNYYIFSLHTWLVLVTTVILIISGTVLIFTIESLSGNSVSLLESLFQSVTSR
                     TAGFNTVEISNLSKATLLVIAALMFIGGSPGSAAGGIKTSTFAVIISMMFMGRNHFED
                     VVIRNRQLPKRTVYQALMVFIFASVIIFFTVVLVSAFDPEKETIAVLFESVSAFGTVG
                     LSTGITPYLTEISKIIITITMFCGRIGSLTIFSIILSKEKNPGRLAEERVLIG"
     gene            complement(18324..19019)
                     /locus_tag="PL056_01615"
     CDS             complement(18324..19019)
                     /locus_tag="PL056_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012189817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPM45743.1"
                     /translation="MKHTILIIEDEKPVRKFLTAGLSTSDYKIIEAEKGHEGIVLATQ
                     YLPDVILVDLGLPDIDGIELIREIREWYKASIIVISARWKENEKVAALDAGADDYLTK
                     PFGMNELQARIRVALRNNSRTGEFRKDGFVFEKEFLKVDFESRSVFINSEKIHLTPIE
                     YKLLSVMIKYAGKVVTHRQLLEEVWGGEKGEDFNQYARVYMAHLRRKIEPDPAHPKIL
                     ITEVGVGYRLTTD"
     gene            complement(19016..20125)
                     /locus_tag="PL056_01620"
     CDS             complement(19016..20125)
                     /locus_tag="PL056_01620"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HPM45744.1"
                     /translation="MKLLAISKIYLATFAIVACCTLLSFLSKDFLTEGDVAMIYLLGV
                     VAVSSRFSWIHSITAVFLSVALTNFLFVPPFYTFHVENIRSVITFIVMFIVGTVVTWL
                     SVSLRKHNEEMKIKEAEKTRFMQQNEENRIRSEKEKLKNDLLSSIAHDLRTPLSSISG
                     TASVLLQKMNDIKDDTRSDMLKTISDEAFRLGRLVENILNITKLESTDFVVKKEWYPL
                     EEIIGSAVSRVEKLYKDRELRISIPDEMIMIKVDPILIEQVIINLLENAAKYSFEKTS
                     ITVYAEVSDNLVVVGVVDKGPGVKETNIPEIFEKFQQFRSNSDMNSGSGIGLSVCKAI
                     IKVHKGLIFAENTPDGFKVAFSLPFDETFKPEEVI"
     gene            complement(20137..>20697)
                     /locus_tag="PL056_01625"
     CDS             complement(20137..>20697)
                     /locus_tag="PL056_01625"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HPM45745.1"
                     /translation="KRFFGDILMDLKNALNSYYEFRKVTDVNCMRLEKMHSRHIKCKK
                     GCSSCCVNLSVFPVEFEAIREEMKKDGFDIGSVIFDENATCGFLKNDLCQIYKYRPVI
                     CRTHGLPIVFLNDDNPSEIFKEVSFCELNFNNFNEYDDDDDGGLIFNDENTLDIDELN
                     TAFFKINQQFCKDPQKRIQLKDLCRF"
BASE COUNT         6525 a         3862 c         4427 g         5883 t
ORIGIN      
        1 ctcttaaagt ttagaataat cgggattgga attggaaaat ggatgttttt atattccgta
       61 ggggttgatc catgtatcaa ccctgttttg catttttccg taggggcgag atttatcacg
      121 ccctgtggtt ggaataatat gtgtcggggg tttgaatgac tgaaagcgag tggattaccc
      181 gaaagacacg gattgattca aagcttaatt ctttaagtca gccgtggaag atcattccat
      241 attcagaaaa tctcgacact tctaaactta ccaatcatgc ggttgaagaa ttccccactg
      301 cgaacggtcc tgccgattat gctttttttg tgaatggcag gtttttaggc attcttgaag
      361 cgaaaaaagt ttcattgagt cctcagaatg tgcttgaaca ggcaaaaaga tattcaatgg
      421 gtgctgaaaa atcaatcggt caatggcggc aatttaaagt tcctttcatt tattcttcca
      481 acggtactca gatctggttt gccgatttgc gtgacgatca gtattattcc cgcgaactga
      541 tgaattttca tactccttca gcaatgtttg aaatgtttga aagaaagtca ggtaaattca
      601 gggattattt tgaaaatgtg ccaaatgata tagaaagact aagaccttac cagaaattag
      661 ctatagcgaa tgttgaaaaa gggatcattg aaaacaagcg gaaaatgatg cttgcgatgg
      721 caactggaac gggaaagact tttacaactg tctcgatgat ataccggatg ataaagtcgg
      781 gttatgcaag gcggattctg ttccttgtcg atcgtcgtgc acttgcggct caggcatcac
      841 aggctttttc agcttttcag actccaagcg gaaacaagtt caatcaggaa tatgaagttt
      901 tcagtcagag attcagaaaa gaggattttg aagaaggtga aaagttcgat atcggcgttc
      961 tgcctaatga atatctgaca aatcccaaca ccgctcatac ttttgtctat gtcgcaacga
     1021 tccagagaat ggcgataaat cttttcggtc atgaaggttc tttcggtcag tctgcaagtg
     1081 atgctgaagc tgatgatgat gcagggaaaa ttgatattcc gatccatgct tttgatgtga
     1141 taatcgctga tgaatgtcac cgtggatata cttcaaaaga cacaaatatc tggcgtagtg
     1201 tccttaatca ttttgatgct ctgaagatcg ggcttactgc aactcctgcc tctcacacgg
     1261 tcgcatattt cggcaatcct gtttaccgtt acactgtgaa tgaagcggtg actgaaggtt
     1321 atcttgttga ttatcaggcg gtaaagataa aatcaaatgt aaggataaac ggaattttcc
     1381 tgagcgaagg tgaaaaagtc gctttgaaaa ataaacagac cggaattgaa accattgacc
     1441 agcttgaaga tgaaagggaa ttcaattcgt cagatgtaga aaggaagatc acttctcctg
     1501 attcaaacag aaagataatt gaagagatag caaaatatgc aaaagagcat gaggaaaaaa
     1561 caggaagatt cccgaaaatg ctgatattcg ctgtgaacga tatcgctcat atttcccatg
     1621 cagatcagct tgtgaatctg gctcgtgaga tttttggacg aggtgatgaa tttgttcaga
     1681 agatcacggg cagtccgaca gttgacagac cgcttcagaa aatcaggatg ttcaggaaca
     1741 gaccggaacc gggaatagtt gtcacggttg atatgctatc taccggtgtt gatattccgg
     1801 ctcttgaata tattgttttt ctccgtccgg ttaagtcgag aattctctgg actcagatgc
     1861 ttggaagagg gacaagaaag tgcgttgata taaacaagga gtttttcacc atttttgact
     1921 gttttgacgg aacaatgatc gaatatttca aagattcgac cgattttgtc attgaagtga
     1981 cagaatcaaa agatgcggtt gatattaagc agatagttga aaacatctgg aataatgttg
     2041 aaagaggtta caacactaac aggctgataa agcggcttag acgagttgcg gacacgatga
     2101 gtgcaaaggc gcgggatgat ttttcaaagt tcattcctga aggagctgtc acgccgttta
     2161 ccgataatct caaagataat ctgagatcaa gtttcaattc aacgatggat attttaagaa
     2221 acaaagaatt tcaggaactt atggaaaatt atgacagggc gagaaatcct ttttatgtat
     2281 cgtactcgac tgttgacact gtaagttcag atctgatgtt caaatacggc gaaaagtcgc
     2341 ttcagccggc tgactatctt gaagcatttt caaagttcat caatgaaaac gaagaaaaaa
     2401 tggaagctct taaagttctt ctcaacaatc cgagaaagtg gaattcagca gcactcaaag
     2461 agatcaggaa cgaaatgaaa agaaattcat tccccgaaga aaaagttcaa caggcacatc
     2521 gtctcacagg acacaaagca cttgccgaca tcatttcaat gataaagaat gcaaaagatc
     2581 acaaaaatcc gctcctcacc gcaagtgaaa gagttgaaac agcgatcaca aaactgatga
     2641 aaaaaagcac attcaacaca gaacagaccg aatggctcaa ttacataaag gaacatctca
     2701 taatcaacct tgcaattgat aaagacaact ttgatctcgt ccccgtattg gaaagacacg
     2761 gcggattagc caaagcgaaa aaagtcttcg gcaaccagtt tgaagaaata attgaaatga
     2821 taaatgaagc agtggcagcg tgatggacaa atataaaaga ggacaagatc atgaaaaaaa
     2881 tcacttttca cggagaaatt acaaacgaat tgggtgtaat tatggctttt tcaaaaattc
     2941 acgaagagct aggttttgaa aagatcgttt gcagttcacc gattgggttt gatatcggct
     3001 cgattcaata caatggaaat gatgtaacag ttgaatttga gcataagtct agtaattttc
     3061 tcactcatgg ccatccatca aaaatggaga aaggaagaaa atatgttgta atttgctggg
     3121 aagatgactg tggtttgatg caaaaaatta agaaagatta taaaaaagat atttttgaag
     3181 ttatcgaaat ccggaaatat gtgaaaattg ataatgaaaa tagtcctgtt gacaataaag
     3241 atgaagaacc aatatgcgga attctctcat acaatcctaa atatgctggc gatgttgatt
     3301 ttgggaattg ggcttttgtg aattgttatc gagttaacac ctctgaaaaa agcaggaaat
     3361 ttaaaaaaaa tgagattccg aaaggatcta aattcttgtt ttacagagat ggttttataa
     3421 ttggaggatt tactgttgtt cgttacgaga taattgaaga accaaaaaca aaaagagaaa
     3481 tggaaatata caaaaaactt actgattacc caataacaca ttacagtctt acagaaaacg
     3541 aaattagtga aaattatcta agaggacata ttttttacac agattttttt gatatgagag
     3601 attacaagaa aaatctttca aaatataaaa ttaataaaca aatgggacgg caaggatata
     3661 taacgataga cagagaaacc tattatagga ttcttggaga gtagtgagtt aaaatggtga
     3721 attaatcagt gaaaatgtga tgaggaatgt tttttcaaat tctcaatttt taagaggaaa
     3781 ttaatgggtt ttggttggtt tagtgaaaaa aagtcaaatt atgatagaaa agattttaga
     3841 agcttaaatt ataaaggcag gtggcagaga aaattcattg acgagtttgt tgaaaagcta
     3901 tttgtatttg aaaatagaga tggtgtttat tccgaaatgt caatctgcaa agaagagcat
     3961 atgcctgaat ttctttataa attttaccga ccgtcatcta acagcattgt aaatttagaa
     4021 aataatttgc tgcatctttc ttcaccggaa gaattcaacg atccatttga cagctatgtt
     4081 tgtattgacg aagacaaatt tgccaagtac cgattactga aaacactgaa ggagaaaaaa
     4141 catatttcaa aggatgaaac tcctgatacg ctatctgaaa aagaatactg ggaaatattc
     4201 tattccacaa cgagtgacaa tcataactac aagaggaaaa actttgattc tttaatttgg
     4261 gaaataaaag ataaaaagtc taaagaattt caaaaagtac tgacttcaat tgaagttgat
     4321 agctggcgtg agtgcaaaga gaaaattaaa catttaagaa aaagctgtta taaaattaca
     4381 tgtttttcaa atttttctga tgaatttaag ctgggaataa acacaacgat gtggtcacac
     4441 tatgcggaca accacaaagg tttctgtgta aagtatccat tgaaattaaa tgagttgaag
     4501 cacaaggatt taattttatg tggactttat ccggttattt atacggcaag aattccgaaa
     4561 gtttcacctc gtgaacttat aaaatcaaaa tttctgaatg ataagcctca gacaaacaaa
     4621 cagattcaga aaactgtatt gaagtctttt attacaaaat ctaggttctg gaattatgaa
     4681 aaagagtggc gtttaattat aagcaatcag gatgattata ttttttcgga cgggacaatt
     4741 ccctttgtta agcccgaatc aatttatatg ggctgtagaa ttgaaaacaa cctaaaaaaa
     4801 catctggtta aatatgcgga taaaaacggg ataaatattt atcaagcgta taaatcagaa
     4861 gaaaaatttg aactgatttt tcgtaaagta aacatggaat ccataaagcg agatgaatat
     4921 gatcagaaat gtcacgacta tttcagaaaa gaagatgagc gagagaaagg gtatcttatc
     4981 cgtaaattaa atgaatttga aaaagaataa ctagctaatg acattcaatc ctgaaataca
     5041 tcaccgtcgt tcaatccggt tgagggatta cgattattcg caaaacggtg catatttcgt
     5101 gacggttgtg gcaaaaaacc gtgaagatct gttcggtgag attgaaaatg gtgaaatgat
     5161 tttaaatgat acgggaaaaa tggttttgca ctgttgggag caaattcctg aacattttcc
     5221 tgatgttgaa attgatgaat gttgtgtgat gccgaaccat tttcatggta tttttttgat
     5281 taattgtaac cgtagaggcg ttggttgtaa gggcgaaaga tttttcgccc ctacagattc
     5341 aggaacatct aaaacggttg gttcaattgt tcggggtttt aagatcggtg taacgaaatg
     5401 ggcaaggaaa aaccttgaaa taattgatgt ttggcagaga aattattacg agcatgtgat
     5461 aagaaacgaa tcagatcttg aaaaaataag gcaatatatt tttgacaatc ccgcaaattg
     5521 ggaaaatgat gaaaacaatg taagggcgaa aaatttttcg cccaaagaaa caataaccga
     5581 tcatgtaagg gcgaaagatt tttcgcccct acgataaaca aaggagaatc caatgtccga
     5641 tatagtaaat aaactctggg gtatgtgcca cactttgcgt cacgatggtg tggattacgg
     5701 tgattacatt gagcagttga cttatctgct gtttatgaag atggctgatg aaaaaagtgt
     5761 taatctgcct gaaaggtgtg actgggaatc gctgaagagt aagtcgggta ctgagttgac
     5821 ggatcattat ctgatggttc tgcagaaact gcgtgatgct ccggggcttc ttggtgatat
     5881 ttttgctcag tctatgccga aattcaataa tccggtgagt ttgaaaaaga ttatcacgat
     5941 gattgatgct gaggactggt cgagtcttgg tgttgatgtg aaggctgctg cttttgaggg
     6001 gcttcttgaa aaggcggcaa gtgagggcaa gaagggtgca ggtcagtttt tcacgcctcg
     6061 tccgctgatt cagtcgattg taaagatcat gcagccagat cctttgaagc agaaagggat
     6121 gaaaatttgt gatcctgcct gtggaactgg cggttttctt gtgtgctcct atgaatggct
     6181 gatcgaaaag acaaaagggg cgattccggt tgatgatgtt cctgcaataa agggaacgac
     6241 ttattacggt caggatctcg gcgcccgtcc tcgacggctc gcattgatga atctgttcct
     6301 tcacggtctg aaacctgtca tctatcttgg tgatacgata tatgagccgg ataaagggga
     6361 aagatacgat gtgattctga ccaatccgcc gtttggcaat aaaggtgcgg gtcaggcacc
     6421 gatgagagat gatttcacaa tttcaacatc aaataaacag ctcaatttcg ttcagcatgt
     6481 tctcacggtt ttgaagccgg gcggaagagc ggcgattgtt ctgccggata actgtctttt
     6541 tgaagataaa gccggcgaag tatttgaaat aatcatgaaa gactgcaatg ttcacacaat
     6601 actccggctt ccgcgaggaa catttatccc ttatgcaaat gctcaggcaa atgtgatatt
     6661 ccttcagaaa ggaagaccga cagaaaaagt gtggatttat gactgccgtt caaacattcc
     6721 gggatgcaca aaaaaggata gaccgctaac tggcgaaatg ttcaccgatt ttgaaaaatg
     6781 ttacggcgaa gacccaaacg gaaattcaaa acgaaccgat cagggggaaa caggcagatt
     6841 caggtttttc aacattgatg aaataaaaaa gagagattac aaacttgacc tcaaatggct
     6901 caaagacgac acactcgatg acccaaacga tcttccagaa cccgcagacc ttataacaga
     6961 agcagtcacc gaactggaag cgatcatgaa agaattgaat gaaatcaatg aattgttgtc
     7021 gtaggggcga catttatgac gcccacattt atgacgcccg cattttatgc ggtatttgta
     7081 ggggttgatc catgtatcaa cccggaggag aacagggcgc gataaatcgt gcccctacgg
     7141 aggaaataat gaattcaaat attccgaaac attgggttga gacaacactg ccaaatattt
     7201 gtaaaataga aacaggaaaa tgggatgcaa atcatgcaga agaaaatggt caatatcggt
     7261 tttacacttg tgcatatgaa ttccagcact gcaataccaa aagattctcc ggtgattgta
     7321 taattttgcc aggaaatggt gcaaatgttg gagaagtgtt tttctattct ggtgattttg
     7381 atgcatatca aagaacttat gtactttcag atttcaagat agatccgagg tattcctttt
     7441 ttcatatgct gtataaatgg agagaaatta ataaagataa acaattcgga tcagcaacaa
     7501 attttataag aattggaaat ttcaatgact atgtatttga aattccaccc ctcaacgaac
     7561 aaaagcgtat tgttgaaaaa cttgatacga ttttgcctaa agtgaaaaat gcaaaagcac
     7621 gacttgaaaa tatccccaaa atcctcaaaa agtttcgcca aagcgtactt gcagacgcat
     7681 gttccgggaa attgacagag gaatggcgag aagggaagga attaccagag tgggaagaag
     7741 ttactttaaa agcaattgca gatataaaag gtggaattac taaagatacc ggaaaacaaa
     7801 aaaaagcaga tatagaagtg ccatatctaa gagtagcaaa tgttcaaagg gggcatttaa
     7861 atttatcaga aattaaaacg ataaaaattc ctaaagaaaa acttgagcaa tatctgctaa
     7921 aacatggtga tattttgtta aatgaaggtg gagatccaga caaacttggt cgaggttgga
     7981 tatgggaaag tcaaatagaa acctgtagcc atcagaatca tgtgtttaga gctcgtttat
     8041 tcgattccaa taatcaacct aaatatgttt cttggtacgc aaatactgta ggagcagact
     8101 ttttctttca atcaggcaag cagacaacag gtttagcttc tattaacaaa acaatgatta
     8161 gtaacttatt gattaatctc cctcccatcg aagaacagca cgaaattgtc cgtcgtgtgg
     8221 agaagttatt tgccatagcg aattcgcttg aagaaaaata taaaactgca attgccagag
     8281 ttgaaaagat agaacagtca gtcctcgcca aggcattccg tggtgaattg gttgcccagg
     8341 atcccaacga tgaaccagcc gaaaatttgc taaagcgaat tttggatgta agggcgaaag
     8401 atccgatctt cgcaaagctt cgacggacag gtctttcgcc cctacgacgg aaagggaaaa
     8461 ccgggaaaac caaatcaaaa taatcaaacc gcatgaagtg ggcgaggaca gtgccgcgcc
     8521 cctacgacgg aaacgaaaaa aaaacagggt tgaaacggtg ttcaacccct acgaaaacca
     8581 taaaattatc accatccttc gactcattcg atgaactcat gataaataac tcaggacagg
     8641 tagcgaatta tgtcaaaagc gtgaaaatca tctgtttttt tagcgtattt cacgattatg
     8701 ttgacaaaag cgtgaacgag aaggacttcg gggtcaagaa atctcgcccc tacaaaaacg
     8761 acaggcaggc agtttcgccc ctacaaattt atttaaattt taaccttgac agaaaacgcc
     8821 ggctacgcac tgaagttcgc cgggacatgg gactccctga caattgaaca tgcagaaaga
     8881 aaaactgccg gcattggtgc attgtgcctg aaagccgggt gctttgtttg catagtcatc
     8941 tgcaacattg gggtcgcaga gtggataaca gtatgcaatt cctgatgagc cctcgatgca
     9001 gtctgagtct cctatgcatt catcttttcc attgcatttt tcaccgggtc cttttgtggg
     9061 tgtaatgcct gacggcggaa gacacatttc ctgtccgaaa cattcaagcc actctggaca
     9121 ttcgccttcg tagcttccgc agagtataag acacactttt tcggtgttgt ggcacgcaac
     9181 ctgctgagtc gtttccggtg gaggagggca gtctgtgttt tcactgcagg gctttagaca
     9241 ctgtccgtat ttggcgttgt catatccttt tacacagttc atcccttccg ggcatgatcc
     9301 ggccatcgca ggatcacatt gccggtatgc accttctcct gacggagcgg tatcgtcagg
     9361 tgtttcgtcc ggtgtttcat ccggtgtttt atcgggttca ggatcttccg actccgcgtc
     9421 ttccgactcc gtgtcttccg actccgccaa ggcttcgtcg gacaggtcgt cggacagatc
     9481 attgttgttg tcagtttcct gagttttatc agtaacttct tcagaatcat cttttgcgga
     9541 gcaggaatag aaaactgaaa gaaaaagcac ggcacatacg agggaaaggg ttcttctgat
     9601 ggacatgata cacctcctat gtttatcccc cgcttggatg agagcatata ttttatgatt
     9661 ttgcagtcaa cttttatttt tctttttatt cagtataatc ccttgagttt atttaggggt
     9721 tttgatgaag aagatcactg atatcataaa gagatcactg aagaatgagc cgacaagaac
     9781 ggtttctatc gatcctgatt ccatcattgc atcccttgct gaatcaacga tactggctga
     9841 aaagaaagat gactggaaat acaggatcga ggatgagata ggtcgtggcg gcatagggcg
     9901 tgtactgatc gcttttgatg aaaaggtggg ccgagagatc gctttaaagg aacttataaa
     9961 ggaagataat ccgggcagtg tgactgtcgc tccgcttgat aagatgaagg cggatgagat
    10021 aaggttcctc cgtgaagcga aggtgacagg tcagcttgag caccccggca ttgttcctgt
    10081 ctatgagatc ggcagaaaac cggacgggaa ccattattat acgatgcgtc ttgtgagagg
    10141 cgtgaccctt tctcaggcga tatatgatgc aggaactctt cagaaacggc tccgtcttct
    10201 gccccatttc cgtgacatct gcaatgccat cgcatattcc cacagcagag gtgtcattca
    10261 ccgcgatata aaacccgaga acatcatgat aggggaattc ggtgaaactg ttgtccttga
    10321 ctggggactt gcaaaagtaa agggtgaagc ggatcaggcg gaaggggagt tgagagaaga
    10381 actcaatctt ctgaaagttg ataatatcgg aaaaacgctg aagggaaaag cgataggaac
    10441 tcctgcatat atgtctcctg aacaggcaaa tggtgagatt tcagaaattg acgaaagaag
    10501 cgatatttat tcacttggag ctgttcttta tgagatcctg acaggacatg ctcctttcaa
    10561 gggacgggat gtgaaggaaa cgcttgaaat ggtcaagttg aataccccgg ttgaaataac
    10621 tatatttgaa cccgaagccc cgcctgacct ctgtgctgtc gtttcaaaga cacttacaag
    10681 agaaaaggag tcaagatacc agtccgcgat ggatgtcgca agagagatcg agaacttcat
    10741 gtcaggcgga aggataaagg catatgaata cagttcgtgg gaacttttca agagattttc
    10801 tgcaaagaac aaactgctga cagggttgct ggcgacactt gctgtggtaa tgatcttcgg
    10861 ttcagtcgtt attttctatg cataccgcga atctgtcgaa aatgaaaggt ccgcgcatct
    10921 gaatctttca ctcggatacc tcgagtatgc agagcgtctt attaaagaga agcgatatct
    10981 caatgccaag atattttccg cagcatcgct ttatcacaac ccgtataacc cgaaaagtcc
    11041 ctggagtttt cctgagtttt tcatgacaga aaatgctatc gaaagttcaa atcagatgct
    11101 ttccgtccaa tctgtgcttt atctttccac cgttcatcag aatgacagct tcaaggaaga
    11161 tcttgctcag atggatttcg atccgagggt gcttgtaatc tcacctgacg gttctcttgc
    11221 cgctgttgca ggcaagacaa gaagaataat ggtctattcg gtctcaaccg gcaaattgaa
    11281 atatactctg aacgggcaca gtgatgagat tgccgctttg gaattctctc ctgatggaaa
    11341 gtatatcgct tcaggaagct gggacagaaa gctcaatata tgggatgcaa gagaggggat
    11401 acttataaaa actattgaaa gctccacagg tgaaatatat gcactttctt tttcaaatga
    11461 ttcaaggttc atagctttcg gagggacaga caggaatctg tggatctccg atataagcga
    11521 ttttcagctt aaggcaagaa agatcatgga acttgaaaat acagtcaggg ccgtcaactg
    11581 gtcgccagat ggaaaaatac tcgcttcaga cagctcggga aatatatacc ttatcgaaaa
    11641 cgggaaactc aaatccgttt ttaagcgaca cagcgaaccc gtcatctctt taaagtttct
    11701 caaagacagc aattatttca tatccaccgg atacgataaa cggtttttag tctggaatat
    11761 caatgcatct gctccgttat atatttatga acactgggac gcttttttcg accttgacat
    11821 ttcaagtgac ggaacatttg ccgccgcggc ttcaagagac ggcactgtaa agcttataga
    11881 tctcagaaac aggcagatag aggatctcag aggacatgaa ggagctgttt attctgtttc
    11941 gttcatgccg gtaggcaacg cgcttatgtc ggtcggtgaa gaccatctcc ttaaagtctg
    12001 gaagactgat gttaaaagac ttgttcagac atatagaggg catgcaacat acataccttc
    12061 gcttaaatat tcgcctgacg gcagatttct cgcttcatca agctgggata acacggtcaa
    12121 actgtgggat gttaacaggg agatgctcat ttcgactttc ggaacacccg ggcttgtatc
    12181 atacagcata gatttttcac ctgacggaca gtttgtcgta acgggtgatt ccgatggttt
    12241 catcaggatg tggaatccgg tcaacgggaa acttctttct gtcatgaaag gacattcaga
    12301 ccagatatct tccctcgctt tttcaagtga cggacggtac cttgtttcag gaagcaagga
    12361 caggactgca aatctgtatg atatgtctgc aagaaagatc gttaagatat ttaaacacat
    12421 aaatccggta cacgcggtgt ctttttcaga aaaaggggat gttatggctt cagcgggaag
    12481 gaacggagag atcaatttct ggagaattcc tgaaggtgat ctgatatttt cttcacagca
    12541 gggcaagacc atactttcag ttgctttctc acctgacggt tcacaggccg tttcagcggg
    12601 agaggatgga actgcgataa aatgggatgt taaaaataaa aagatggata aaaccattta
    12661 cggacatacc ggagccataa acggagttgt gttttcaaat gacggatcgc tctttgcaac
    12721 agtcggaaga gaagtgatca ttcataatgg aaagacttct gaaaaacttc ttgcctttga
    12781 tcttcagtat aacggctaca gcattgcgtt tgaaaaggac ggcacacatt ttgcggtcag
    12841 tgacggtgct ttgatcaaaa gatatccggt tatccttgaa atgtggaagg aagatcccgt
    12901 caaaatgctt gaagaagctg agcacaaggc agggaaaaaa cttgaaggat tcaaactttc
    12961 accaatatca gaaaaaataa ccgtcacaga agaaacatca gaataaccta aaacgaaaat
    13021 ccggcactca gcccgtaagt tcttgaaaca ggatcgataa tcggggaaat gctgtttaag
    13081 gttaaagcgg gttctgctcc gagtcttttc caacttgatg aaatggaaag gagggttatt
    13141 ccgcctgaaa ggatcagtac agcgggaacg atggaggaaa tacccagaat aaagaaaccg
    13201 acactggcaa atgcagcacc cattgaaccc attgcttcat ccatagtctg atcgtcactc
    13261 tcttctctgt tttctttgtc aatttgtgct gatccgacaa acccgccgat tgcaagtgca
    13321 aacaatggaa ttgaaatgga tgtgagaatt atcccgtttg tccggaattt ataagaggca
    13381 ttcttcttta ccgatctttt tatgcccgaa gtttcataaa attgatatgc atcaatatca
    13441 ggatgatcgc ctaatatgct gattttattg aattcttcag aggtgagcag tttgtagtca
    13501 gcagaagatc taagtatcaa tccgcttgat atcaatgtca atccgatgga ccagaaaggg
    13561 gcagccagaa taattccacc acctcgagtt acgcctaaaa atacacatcc tgtcaatatt
    13621 gatgccgctc cgatagcaat taatgaatat ccgcccgcgt tttcccgttt gtaagttttt
    13681 ttatattttt gtgcatattt gacagctgat tcccattcag tgttacctga tgttttcatg
    13741 gttttgagat tagtcccgct ttctgttccg taaagagttg tgtggttaaa cccgagaaag
    13801 gcaacaagaa aaattataca ccacaaaatt ttcatactgc tcctcctaaa acgaaaatcc
    13861 ggcactcagc ccgtaagttc ttgaaacagg atcgataatc ggggcgacgg aatcaagggt
    13921 tagaagatag tgcgtttttc cggccttttc atgcctggcg gctttggcaa gaaggatcgt
    13981 tccaccgaca aaagtggcta ttcctggagc cagtgaaaat atctgaagaa agtgagcaaa
    14041 aaaggtgtca agacctgtgg ggtcccagtt gccatgatct ttgtttgatt cttttatgcg
    14101 attgtagctg tcaaaaaaac cgtaaacagc aattccgata agaggaattg atattcccat
    14161 cagtgcaagc ccgtgttttt gataattgtc agccgtagcg atattaaaat ttctttgatg
    14221 ttttgaattt tcgaaatatt cttcaggagt tgttccaggg tcaccggaat attcaagaga
    14281 tatcttgcgg taaacataag ccctgttgta cgcatttaca gcaccggaac tcatcaaagt
    14341 ccctgccgca atgaacgaaa gacctcccaa tgtgagacca tactgcagcc ccatcgaata
    14401 ttcccttttt gaaagacggt caacgacata aagactgatg cctgccgcag ttgctatcgg
    14461 tccgagcgaa aggaaaacaa tggagaatgc tttaaatccc tcatatcttt tgctgtatct
    14521 ggcagaatcg gaaatggcat actgctgaaa accgtgatgt ctggaattct gtgccttgag
    14581 agtgacttca ttgtatttct gagtcattcc cgctttttta taggcaagcc cggcaagata
    14641 gtatttctgt tcatattcat aaatttctgc aagtttcaca tatacatttt tgtcattatt
    14701 ccagcattca agatgttcct tgagaagtct tgccgcctgt tttgttttcc ccttttttat
    14761 ataaccgtca gctctgaaaa ccaatttgct gtaagattca ccgtattcat agcaatcttc
    14821 tgcatacacc tgatgggtga aaatgatgat cagcagaaaa agaacagaaa gtttaaagtt
    14881 cttcaaaatc ttacctcagc tctcgtttca taaaatcact gttttgtgac agaaaaacaa
    14941 cttttttatt ataattattt aaggaataag ttcaattaat gttatttcgg gcattgaacc
    15001 gaatctgaca ggaggtcccc atgtccctgt tcccctgctg acatgaagga tcgacccgct
    15061 gttaaatttg tagttcccgc tacagtattt gaaagccatc cgcacaaaaa taccgaaagg
    15121 gaatatctgt ccggcatggg tatgtcctga aagcataaga tcaaagcgat cagttgcccc
    15181 tttttcaatt ttcggctgat gttttaaaaa gatgttgaac ctcgaatgat gggctttttt
    15241 cagaatatca agatcattgg gagcggacat tccgaatctt ttccctgtct tatctgcaac
    15301 tgctgaaatc gttatgtttc tccacacatc aatgatctga ttgtcaataa gcttaaatcc
    15361 gtttttttct aagaaattct ttgaattatt gaaatcattg atgaattcgt ggtttccggt
    15421 tattgcgaat ttcccgagtg gggcgggtat cgagttcatt gcctctgcaa ttctttccgg
    15481 ctccatcatt ccgtgatcga ggtaatcacc ggtgcttatg agaatatccg ggttcagttc
    15541 cgttataatt gaagcgatct tttgagcaaa ggggagtccg gtcgtcgctg aaaaatggat
    15601 gtccgtaacc tgaacgatcc ttgtatattt ttcaattttc ggatttttta ttttcagagt
    15661 caccacctgg atatcttcgg caagaaagaa gcctgttgcg acagtatgga acgagattag
    15721 gaaaaggaca atgaaaacaa gccgtttctt gtgtaacgga agctttttag agtagatgtt
    15781 cttaaacagt tttcttatca ggatcaaaag ttcgacaaaa agtccgtaaa ggaaaaaaac
    15841 gaacatgaaa gtaaaaaatg cataaagaac gcaactgaat atggaaagag aaagagagcc
    15901 ctgtaaagaa cggttggaaa aaagaaaaaa tgaaagaaaa atagggatat agagcagcat
    15961 gagttcccgc ctgtatttga tcattctgcg ggagccgccc atcatggaat ggatgaaata
    16021 acggatcatc agtatggaaa ttgaaattac aaccgttaag aaagttaaaa atgaaaggat
    16081 gtttgtcact cgcacctcct gaaatgcggt aaatgatact ttaaaaagat atcagggtca
    16141 aagttttgat tgaaaaaatt atggaatcag tgcgttttct ggttgtatgc ctcaattgca
    16201 tcttttttcc cgagaacgac cagaatatct gaaattttta ttttaaaacc ggcacttggg
    16261 gaaatgacca tattgggagg cacaagctct tttatcgcaa ttattgaaag attatgctcc
    16321 tttctgaaat cgatctcttt taaagacttg ttcgccatag agcttaatgg tgcaagttcc
    16381 gcaactgaat aatcttccga aaggggtaga aaatcaagca gattgttgct catgagcttt
    16441 gttgcaaagc gttgccccat atccttttca ggaaatatga tgttctttac ttccatcatt
    16501 ttaaggatcc tttcatgttc ctctgatata gctttcacat agacattttc aattttcatg
    16561 tctttgaggt aaagagccgc tgttattgaa gattcaatgt ctccgagagc aatgataaca
    16621 aaatcggcat cagcggggac gatctgatat aatgcgtttt tctgcgatgc atcggcaatg
    16681 atcgctttca ttacaaagtt ttttattgaa tctatcttgc cggcatcaat gtcagcgaca
    16741 atgcagtcac aaccgcattt tgaaagactg agagccagat gcagtccgaa ttccccaaga
    16801 ccgataacaa cggcagttct gttttttttc tttccgaaca tattcacctc ttttatccta
    16861 tcaacaccct ttcctctgct aatcttcccg gatttttttc ttttgagagt attattgaaa
    16921 atattgtaag cgatccgatc cttccgcaaa acattgttat agttatgatg atctttgaaa
    16981 tttcagtgag atatggtgtt attcctgttg aaagaccgac tgttccgaaa gctgaaaccg
    17041 attcaaaaag gacagcaata gtctcttttt ccggatcaaa tgccgaaaca agcaccactg
    17101 tgaaaaaaat tatgacagaa gcgaaaatga aaaccataag tgcctgataa acggttcttt
    17161 taggcaactg cctgtttctt attacaacat cttcaaaatg atttcttccc atgaacatca
    17221 ttgaaattat tacagcaaaa gtcgatgttt ttattccgcc tgcggcagaa ccgggggatc
    17281 cgccgatgaa catcagagcc gcaataacga gcagtgtcgc cttggaaagg ttcgatattt
    17341 caactgtatt gaatccggct gtcctgcttg ttacagactg aaaaagagat tcaagtaaag
    17401 atacagagtt tcctgaaagc gactcgattg taaaaatgag aacagtacct gaaattatca
    17461 gaatgactgt ggtcacaaga acaagccatg tgtgaaggga aaaaatataa taattcctga
    17521 gtctggtctt tttccgtctg ttaaagaaaa gtcccttgag ttttgatttt atctcataag
    17581 tcacaagaaa tccgatgccg cctgcaatta tcagaatcat ggtgataatg tttacagaaa
    17641 tattcccgcg gtatctcatc aaactgtctg aatatgttga aaagccggcg ttacagaaag
    17701 ctgaaacaga atggaataca gagaaataaa gccgtttaaa caggttgttt tcatcaagag
    17761 tcataaaaaa aagaaacgct cctgaaattt caaaaataaa agtatattta ataatgaatc
    17821 taaggagttc agataattta atatcagctg cgccctgcat gaaggactct tccatcatca
    17881 tccggcttga caagggaagc tttctgttga acgcaaaaat aaacgatgct gcgaaagtca
    17941 ttatgccgat tccgccaagc tgaataagaa caagtatcac tatctgaccg aacattgtga
    18001 aatgaaccgg agtatctaca acaataagcc ctgtgacaca aactgcggat gtcgcagtga
    18061 acagtgcatc aacccatgtt gtaccggtgt gtgatgcgaa agggagtttc agcaggagca
    18121 ttccggaaaa gatcagcata agaaatgaaa ataccagaat gtgacttccg gtaatttttc
    18181 tgttattcat aaatacaaat cctcatcctc aaaaaaaccg gaaaagtata tttggcagat
    18241 gtatatatag ttataaaaat aatcttaaaa tagagacggc ttgtgttaaa atcgtataaa
    18301 aataatggtt aatgccaatg aaatcagtct gttgtaagcc gatatccaac cccgacttct
    18361 gtgataagta ttttaggatg cgccggatcc ggctcgatct ttcttcttaa atgcgccata
    18421 tacactcttg catactgatt gaaatcctct cccttttctc cgccccaaac ttcttcgagc
    18481 aactgtctgt gggtcacaac tttaccagca tattttatca ttaccgaaag cagtttgtat
    18541 tcgatagggg taaggtggat cttttcgctg tttatgaaga cgcttcttga ttcaaaatcg
    18601 actttaagaa actctttttc aaaaacaaac ccgtcctttc tgaattcacc ggttcttgaa
    18661 ttatttctga gtgcaaccct tattcttgcc tgaagttcgt tcataccgaa aggttttgta
    18721 agataatcat cggctcctgc atcaagtgcg gcaacttttt cattttcctt ccatcttgca
    18781 gaaattacaa tgatcgaagc tttataccat tccctgatct ctctgatgag ctctattccg
    18841 tctatgtcgg gaagtcccag atccacaaga atgacatcgg gaagatactg tgtggcaagc
    18901 actattcctt catgcccttt ttcggcttca atgattttgt aatcacttgt tgaaagccct
    18961 gcagtaagaa atttcctgac cggtttttca tcttcaatta taagtattgt gtgtttcata
    19021 ttacctcctc cggtttgaaa gtttcgtcaa aagggagtga aaaagcaact ttaaacccat
    19081 ccggagtgtt ttctgcaaat ataagccctt tgtggacttt tattattgcc ttgcagacgg
    19141 aaagtcctat gcctgagccg gaattcatat cgctgtttga gcggaattgt tgaaacttct
    19201 caaatatttc cggaatattg gtttctttaa ctcctggacc tttgtcaacc acgccgacaa
    19261 caacaagatt atctgaaact tcggcataaa cagtgatgga ggttttttca aaagagtatt
    19321 ttgctgcatt ttcaaggaga tttattataa cctgctcaat aaggatggga tcaactttta
    19381 tcattatcat ttcatcaggg atcgagatcc tgagttccct gtctttatag agtttttcaa
    19441 ctcttgaaac ggctgacccg atgatctctt caagaggata ccactctttt tttacaacaa
    19501 aatcagttga ttccagcttt gttatgttca gaatattttc tacaagccgt ccaagtctga
    19561 aggcctcatc ggaaattgtt tttagcatat cgcttctggt gtcatcttta atgtcattca
    19621 ttttctgcag aagtacagag gcagttcctg aaatggaaga gagtggtgtc ctcaggtcgt
    19681 gggcaattga acttagaagg tcgttcttta acttttcctt ttcagatctt attctgtttt
    19741 cctcgttctg ctgcataaaa cgggtttttt cggcttcttt tattttcatt tcctcgttgt
    19801 gtttcctcag actgacagaa agccatgtga caaccgttcc aacaatgaac atgacaatga
    19861 atgttataac cgacctgata ttttcaacat ggaaagtgta aaaaggaggt acaaaaagaa
    19921 aatttgtaag agcaacactt aaaaatactg cggttattga atgaatccat gaaaatctgc
    19981 ttgaaactgc tacaactccc agaagataga tcattgccac atcgccttca gtaagaaaat
    20041 cctttgaaag aaaactgaga agagtacagc aggcaactat agcaaaggtc gcaagataaa
    20101 tttttgatat agctagtaat ttcaaatcat taccagttaa aatctgcaaa ggtctttcag
    20161 ttggatcctt ttttgaggat ctttacagaa ctgttggttt attttaaaaa atgcagtatt
    20221 cagttcatca atgtccagag tgttttcatc gttaaatatc agtcctccgt catcatcatc
    20281 gtcatattca ttaaagttat tgaaattaag ttcgcagaaa gagacttcct taaatatttc
    20341 tgatgggtta tcatcattta agaaaacgat tggaagtccg tgagtcctgc atatcacagg
    20401 tctgtattta tagatctgac agaggtcatt tttaagaaat ccgcatgttg cgttttcatc
    20461 aaatatgacc gatccaatgt caaagccgtc ctttttcatc tcttccctga tcgcttcgaa
    20521 ttccaccgga aagactgaaa gattaacaca gcaggatgag catcctttct tgcatttgat
    20581 atgtctgctg tgcatttttt caagccgcat acagttgaca tcggtcactt ttctgaattc
    20641 ataatagctg ttaagggcat tcttcaaatc cataagtata tctccaaaaa accgttt
//
LOCUS       DAOOYB010000014        20653 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_2432_length_20653_cov_9.409846, whole genome shotgun sequence.
ACCESSION   DAOOYB010000014 DAOOYB010000000
VERSION     DAOOYB010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 20653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20653
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2432_length_20653_cov_9.409846"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1007)
                     /locus_tag="PL056_01630"
     CDS             complement(<1..1007)
                     /locus_tag="PL056_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45746.1"
                     /translation="MNKNSNIIVFQALLLFLFWSCTSCNGNNPENDTENDIDMTRPDK
                     DSDDLSDEALAESEDAESDETDDEVEYADVDYQGAEECPELKVAKFPYYNDDLSIHFC
                     RKCDKPTVKDPQCVENLWKDAAAALYAVAPEAECESGYPCDMADLKPQTQAEWDAALE
                     GLPSLPYRPHECDIVLSARSISTGGKWATDSTAGAIKHFDISDGKVGLFLKNVVLDYK
                     KYQTYSKTMEYDPETRKYRALSPDLLETGSYNKGCMLHIVNNRHIYSEENTFRSYLTY
                     SCSDGNKRVVYPRNINFVSYTPALSEKWAIANIQEEDGGIKYTMYAKVGTWKWTKLME
                     G"
     gene            complement(1082..>2005)
                     /locus_tag="PL056_01635"
     CDS             complement(1082..>2005)
                     /locus_tag="PL056_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45747.1"
                     /translation="EPLLQFELIKEITEYVVKIADKKGFAPHLSIATNGTLINEEVLD
                     FFEKYKFRVELSLDGDEFSHNLNRPMRDGGNSYRSVVRNLAALKKRAEHLAVVGVITP
                     LTSAHIAENAKHVMDELNINSYIMAADYTAPWTEDDLSKVIEQFLILEEWYLAKSRNG
                     DSFYFSAFDAHIAAHIKGGFKPGDFCDVGRKICAVGEDGRIFPCVRFAGKPERAANYV
                     LGDVNSGFDWKKCSEVSTENHSPRTQCSDCSLDGRCITYCPCLNWDTTGSMTQVPGIL
                     CAFESMVIPVADRIARTLFKEKNRSFIKKHY"
     assembly_gap    2006..2105
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2106..2521)
                     /locus_tag="PL056_01640"
     CDS             complement(<2106..2521)
                     /locus_tag="PL056_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dickkopf-related protein"
                     /protein_id="HPM45748.1"
                     /translation="MSGFSVKPVKNYKPGYPVKGFFRRSFVIAVPIKLFSLLVVGFLF
                     FITVPGCFEQEMRTDGVQVPDFDTVTDNDNSAPDEIIGLPDPPDDSFLDNDTADIETN
                     DFDQIDGGAVPECLEDKDCEEGYYCDLTTYTCVEQL"
     gene            complement(2570..3685)
                     /locus_tag="PL056_01645"
     CDS             complement(2570..3685)
                     /locus_tag="PL056_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45749.1"
                     /translation="MMRKNITFFFLILFIFLFAYSTENKDKPVELESVFFNENHVSCQ
                     KYCAADPECVKCVKSSTCDFKFEKIKTFKGPGRNYTACKPVKSENYEECVKYCDSNYR
                     CDHCDSTMNCGVSFVNIKSFRGKGTNWYACEKIRDINSFWASKVKIKPEHRYLVVANG
                     GYGGLFGHDGIEWFCEKYLTPEIAPEALCIGAYGRPRTATWMLSENIKNTVMLMEKVT
                     GVRPQVIMLGKSMGGCKLHHAVAGEKGGSRGGLEKLDISLFVGVDMSCQVKRHFEDPE
                     DILEFKSNIKELWNFYQLNVDTSQTGHRAHFEGQPFDDSIHINVNVESFDTDKNAKIV
                     KPEKPLCENAAHLNVDECVPLLETIQKLVLKKMGKIK"
     gene            complement(3682..4281)
                     /gene="pth"
                     /locus_tag="PL056_01650"
     CDS             complement(3682..4281)
                     /gene="pth"
                     /locus_tag="PL056_01650"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HPM45750.1"
                     /translation="MLINKSGIVLVAALGNPENKYLKTRHNAGFLFADSIAQQFNFSP
                     FSYCVKMEGHISVGNISGKTVILLKPSTYMNRSGISVLKAMNAFNVSHEKLIVIHDDT
                     DINFGSHRIKKGGGDGGHRGIRSISGEIGTPGFARIRIGIGAPLKNLPLEKYVLSDFL
                     DDELNLINNSISSKWKDILLKMIGEGVEESMNAFNRRNG"
     gene            complement(4290..4907)
                     /locus_tag="PL056_01655"
     CDS             complement(4290..4907)
                     /locus_tag="PL056_01655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HPM45751.1"
                     /translation="MKTFKLIVEKRDQGKKSVKAVRTNGSVPVEIYGKFMESNISGAV
                     SRKELVKGLHTSQGKNIIFDLEYEGKSILAVAHDMQIHPVKTNIIHVDFIAVKEDQQI
                     VVTVPVTRSGRSLGEIAGGKVFQVLKEVKVSCRPADIPSEINVDVTEKEIGDRIKISE
                     LPYPQGVTPVFHQDSPVIVVNKGRGQSATEEGEEEKPAAEKSAAE"
     gene            complement(4929..5003)
                     /locus_tag="PL056_01660"
     tRNA            complement(4929..5003)
                     /locus_tag="PL056_01660"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4969..4971),aa:Gln,seq:ttg)
     gene            complement(5004..5870)
                     /gene="ispE"
                     /locus_tag="PL056_01665"
     CDS             complement(5004..5870)
                     /gene="ispE"
                     /locus_tag="PL056_01665"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HPM45752.1"
                     /translation="MYLETKTPAKINAFLKITGKDEVSGMHYIESLMIPVNLYDVLKI
                     KESPEFEIETSGINEVIPKEKNIIFKAFNSFKSYINMPVPDFKIILEKSIPTGAGLGG
                     GSSNAAGFIMFLNKYLGLDLSLKRLNEIASSVGSDVPFFLYGSPAFISGKGEIVEPLK
                     LERVNKYLLLLYPEIIVNTRNAYALFDKKKLTKSGSVNINSVRQTGTGSLKDWTKSIF
                     NDFEDVVFEEYPLLADVRNRLMLSGAEKVFMSGSGSSLIAIFGTDKARENALDEFSDK
                     FATVRKIELLTG"
     gene            complement(5861..6349)
                     /gene="coaD"
                     /locus_tag="PL056_01670"
     CDS             complement(5861..6349)
                     /gene="coaD"
                     /locus_tag="PL056_01670"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007086244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HPM45753.1"
                     /translation="MDKKAIYPGSFDPFTLGHLDIVKRALKIFDHIYIAVGENKNKTT
                     LFSTEERKEQISEIFKNNPKITVTSFNELLVNFAKKINVFTVIRGLRAVSDFEFEFQM
                     ALTNRVIEPEFESIFFMTSEKYSFLSSSIVKEIASKSDTELSQFLTPDVEKALRKKLK
                     CI"
     gene            complement(6352..6909)
                     /gene="rsmD"
                     /locus_tag="PL056_01675"
     CDS             complement(6352..6909)
                     /gene="rsmD"
                     /locus_tag="PL056_01675"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HPM45754.1"
                     /translation="MFLRISSGSLKARKFRVPSTSLRPTCEKVRAAFFNSLFSVINFE
                     DRKFLDIFSGTGAFAFESVSRGFKSACAVEVDSKAAEQIRSNSKELGLDDLVKVINGD
                     VFSIDLNCFSGMKFNAIYIDPPYSMGEKIPSLLERILSSDIIDEVCVICVEGSSLTEW
                     SRHGWTNRNKKFGDTHLSFFYSWED"
     gene            complement(6912..7874)
                     /locus_tag="PL056_01680"
     CDS             complement(6912..7874)
                     /locus_tag="PL056_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45755.1"
                     /translation="MKFICNNCGFSAEVPDRRTTCPMCASDNVSVVSMTEQLATPEKP
                     VEKKEADPVQKEQAEQPVGKDQEKKGRGPTERITLNDEFFSSKPDREQQEIANIIKEL
                     YPEAEEKGRAGFKMPDRKILIGAAAVAVLFIVVLIFALIPDDKSGEDNVASINETEEK
                     NPAVPDEEDIEDIDEENIEPIYKKEIVKPAENVEKKQDEKQIVEKQVPVLKKKEARKQ
                     QPKVVKKAETVRPQNDSKGTFDSYVKAGHKALADKKYTDALHEYKNASRINPTNGPVY
                     KFLGIAYAYLQNQKQACVNYKKYIQYSPDAPDRAQVEAFLKACQ"
     gene            complement(7878..9407)
                     /locus_tag="PL056_01685"
     CDS             complement(7878..9407)
                     /locus_tag="PL056_01685"
                     /inference="COORDINATES: protein motif:HMM:NF041621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MXAN_5187 C-terminal domain-containing protein"
                     /protein_id="HPM45756.1"
                     /translation="MFSNLGLKLKVFFLMAVVISALSAVNFTLFRSFIAKIDIQQGPK
                     NSVKLVEEKVKNTTYSLIANSFNVARQLKFEKASRNAPVRIAEIKSLTEYVVVDQNGD
                     FVAGDIKLMSSQKGIPAVERALKEGYASDGSIFVNDTMYLLGVVPMMTEVPGEGQKLF
                     AAVSVKKLTLAFNVNDFPMPVRVFMGDKFITETMNSKWSEIENSCGNEKVKNAVEDLI
                     KSQGQKELNRWTHVHSYFMPYDMLGGEKVVFITLVSTMPGWEDYTKVVLYSLLYTLAG
                     IMITLFFTFIVTHTIDKVFRNLAADISVMKVGEKLVMKKYSHGADIAVSALNTLVSKY
                     LRHFENADASSGPIGNAGLRREDAVLNKKSDFESQIPDPFGVDDIENTPRKPVQALSE
                     NRSTVKSTAKAPPPSHFDSDDNEKTQIMEVGSKEGLITNSIAPEPEPLDPMEQLWLDY
                     CKVKERNGETVTENEKRSFIGKVRTNRASIMAKYKCSDVQFSIEEKEGKPVIKAKPLN
                     G"
     gene            complement(9438..10175)
                     /locus_tag="PL056_01690"
     CDS             complement(9438..10175)
                     /locus_tag="PL056_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45757.1"
                     /translation="MNNLKRTTCFVVVIVFLILHPFNAEAGMLKLLVSYKKGIEKAGH
                     KTTEIVDGYWRFPVLEVPKPLEREKYSFVIEGYQTIKNVNETLELIGAGISRSNILLP
                     TEGVLTIENREDFPRKINILKEGENSEKVELTVPAKGSITHVFSSSGDYTLVDTLFQW
                     NTVYVRVLQSTYLFVVNEGSSRIEIPDIAPGTYTVRVYYGIRWVYQEDFVMISNAAQN
                     IGYRIEEGTVYSINSTSYSTTISMPGD"
     gene            complement(10177..12270)
                     /locus_tag="PL056_01695"
     CDS             complement(10177..12270)
                     /locus_tag="PL056_01695"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPM45758.1"
                     /translation="MANTNPGRFLLKGKKNDTEIQQFEDLIKKRPDDDRAYVRLAELY
                     ARAGNEDKAIELYEKAAILFEKKGFLNKAKAVLKQALMINSEHGKINVLLADYDRQSG
                     LIKDATMRYQTAVNYYAKIGNKLAAINILRKMIEMFPGNINFSIKLGNMLVAEKMYHE
                     AEKLLLPVAESLKGTDKVNEYAGVLKLLYTATNSESAIGRDLVNLYLKSGSYSNALAV
                     LQKLIVDTPDSIEFLEKLAFVFEKLGETKKLISTYKQIAVACAQRQDIPERDRIYRKI
                     LDIDPNDREALSLLKEEGKLRDLISDKIDSSMMNMGEEDEHIDLVLDIDIDADETENE
                     KDEHQVTETEESAPVIDIETAIKEAGVFLSYRLFNKAIDKIQSCKEWQTSPEAIDVLI
                     QANIESGEIETAGDLIMSLIDLKISTGAYQEAKELLVDAENIVGTDDPRISKRQGLIV
                     EHMRDLDSDIQNNDIFDGIPDLPQKETVSAVSENEDEDELVELIEQEERSEPFELTNV
                     ADEQSGTEKSEFENMTMDILSELQEPPQVQLDELEFYISIEDFASATQLLQELLINYP
                     DSRFLSGIKDILPVRKEDNLSDTMKEMKNVLENSSEYGGGTEDLYDMALSHLSMGMFS
                     EAITLFNKILSADSENLACLTGLAQAYTMADKPDQAVKVLKEAEKLTEDDEILSSIKE
                     QIDELENSKKSRSKR"
     gene            complement(12288..12851)
                     /gene="efp"
                     /locus_tag="PL056_01700"
     CDS             complement(12288..12851)
                     /gene="efp"
                     /locus_tag="PL056_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013886928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HPM45759.1"
                     /translation="MAIQTSEFRKGLRIEIDGIPYQIVELQHIQQKRRAVIKAKLRNV
                     LSGSIAERTFLSGERVDKPDLEEKVMEYLYTDGGHYYFMDSESFEQISIDEKVIEDAI
                     PYMKENEKVTVLFYNGSPISLDLPQFIELEIVETDPGFKGDTVTTSYKPAKLVTGGQV
                     MVPLFISTGDVIRISTEEFTYIERVKK"
     gene            complement(12928..13380)
                     /locus_tag="PL056_01705"
     CDS             complement(12928..13380)
                     /locus_tag="PL056_01705"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF013392.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HPM45760.1"
                     /translation="MKICIIEGPLLDKVGTREPSVYGSFTRDDLVRTTLEKAGQLSVS
                     IEFMNSYLEGELAGLIAGCKCDGMVLNPGAYTHTSVLIRDAALCSGIPFVEAHLTNIF
                     GREDFRHRSFLSDIASAFICGLGIYTYASAVEALYLILKNYENRSSKT"
     gene            complement(13377..13598)
                     /locus_tag="PL056_01710"
     CDS             complement(13377..13598)
                     /locus_tag="PL056_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45761.1"
                     /translation="MITETMVLIALKQKNWMQAESMSREIASEKGSDVMKVINGASKA
                     AQSRPDAAYLLSLWAQIRWNEINDDEDFI"
     gene            13723..15285
                     /gene="purH"
                     /locus_tag="PL056_01715"
     CDS             13723..15285
                     /gene="purH"
                     /locus_tag="PL056_01715"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006002013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HPM45762.1"
                     /translation="MPVISSAIISVFDKRGLTAFVSELHKMGVSIYSTGGTHKMISSE
                     GISAVKIEDYTGFPEMMDGRVKTMHPKIAGGILACRNDEKHMKSARDHGIKMFDMVVV
                     NLYPFKNTVEKGGEMADIIENIDIGGPTLIRSAAKNFKDVVVITSHEDYAAVIEEMKN
                     NNGAVSIETKFRLATKAFSHTALYDGYISSYFSKIDHKGEILNEDPGIITLQFTKKEH
                     LRYGENPHQKASFYVDNSGITGTVASARQLHGKQLSFNNYMDLESAKNIVADFDEPAV
                     AIVKHTNPCGAAIGKTLKAAYESALACDPVSAFGSIIAVNKELDAETAMVMKELFVEA
                     VIAPSFSEEALEILKKKKNIRLIETGTFAKRPDMDWELKKVSGGLLIEDADRRIITEN
                     DLQFVTEKKPSAEEIKELLFAQNLVKHVKSNAILLSKNGATVGIGAGQMSRIDALEIA
                     IKKANLPVKGCVLASDAFFPFRDSVDMAARHGIKAIIQPGGSVKDEDSIIACNEHGIS
                     MVFTGIRSFRHL"
     gene            15307..16974
                     /locus_tag="PL056_01720"
     CDS             15307..16974
                     /locus_tag="PL056_01720"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HPM45763.1"
                     /translation="MKTYSINLHLPENIIESIEVKQRTTFLDLAIKYQKSFDCKIVGA
                     VFNNELCELKKEVSDNGNLELITLRDKDGYLFYLRSLFFILIKAASEMFPHATLHIEY
                     SIGNGYYCWFEGLEWIEPGEIEKLEARMKEIVKADIQFSRRRVPTWSAIEEFRKNGLM
                     DKVMLFKIREEKCTSVYMLESTIGYFSGYLVPSTSYCDRFSISFYNKGIVIVAPDPSN
                     PSVLPAFKDSPKYFNIIKEHAEWLDILEMDNCAKLNDLIREGKSRELILLSEALHEKK
                     LAKIADEVYRRNKVIKIVSIAGPSSSGKTTTANRLAVQLRVNGIKPFLISLDDYFIDR
                     DRTPVDKNGMRDFESISAINVKLFNENLDDLLKGHEVELPTYDFKTGRSMKSGRKLTL
                     PENGVVVVEGIHGLNPDLYFNVPDQKIYRIYVSALTALNIDNHNRISTTDERLLRRMV
                     RDNKYRGHTPVETIRRWPAVRKGEEKNIFPYQENADIMFNSSLVYEFSVLKKYAQPLL
                     ENIEKSTPEYREVKRLLKFLSFFEEMDDSIIPPNSILREFVGGSVFI"
     gene            17051..19360
                     /locus_tag="PL056_01725"
     CDS             17051..19360
                     /locus_tag="PL056_01725"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPM45764.1"
                     /translation="MTFLRTIIFSALFSAPLLMFPADEPADKPVKEETPVETVQKAEK
                     IEVKGTLLERGTRAPVSRKVFYIIEKDNKKIRTDKEGGFSFSLAEGTYTLSFPIVGYE
                     KFETKIEVKKGEKLELTIRLEPLVINPYKIVVTGEKNKGEVSVQRITIEEASKIPGTN
                     RDVLKAVTSLPGVTNISVFNGIGSGLVVRGSSAGDSSTVVNEHGIPLMYHFGGLDPVI
                     EPELVESLDFFAGGFSSEYFNTMGGVVQINLRDPRKDRWGGYVNLSLLNVSFMVEGPI
                     SKKDYLAVAFKRGMLDAYVKIVEELGFFNNSVSFSTYPFYYDAMILYVHEFNKRNRLK
                     MYTVGSWDEVRLGFDNQTSIQKMSNTAKTKTQFVQHTTEWHFRENDFKSLFSPMAEVV
                     WVDINAGDAAYFKNFFFQLELSEKMEVKLNDIHKLRFGAKFYYGFYTMDVNLFAMPKE
                     GEVAYNPLSNEIEDHSEGHYWFPGFYFMDEITYNGLTVVPGINFIYDEHNTQMMFDPR
                     LTIKYKINDQWLLKTSGGLYSQLPSNDESYEPWGTPGLRPEKALHAIVGVEYNPLDFL
                     EFDLQGYYKHFFDLVRRDDSFDLSSFSNGGIGYAYGVEFLIRHKMTEKFFGWISYSFC
                     MSERKDSPDSDWRPFDVDVNHNLIAVASYKLNKYWQFGARFKLASGAPYSNLMFTDYS
                     FDADNNFNIPQYRGDVNDSRLPITHQLDLRIDKYWLFDNWIFSTYLDIQNVYMAKNVL
                     GVSYRNDYTEKVYTYGFPIMVFLGFKGDF"
     gene            19360..20310
                     /locus_tag="PL056_01730"
     CDS             19360..20310
                     /locus_tag="PL056_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45765.1"
                     /translation="MRKYLILLVFITAVSCEPNPEVKPGYIVGDIRPLGMKAEPVEAV
                     PGDMITLSMLIGGRTFSQGSDLKISWLGLEELTIPYTKPLIFPVPENLEELMPADSDN
                     PQASLFIDNFNKNGFADLPVTASFEIPVQGGPETRTLTVTKTVRIYKEPPEDGIRKNP
                     VINHVLASYEIYGSVKSFEIEDGGFISFSISQVPETIIFRSIPLSQEETGWDRLNFAW
                     FFTSDTGELLENDMEVELDRKKYGDAVPEDQKATQNREYFALSFKPVLEEIEESKIKL
                     PVKFNFYHIIRDKAIDAQSSADYRWGQDFMWFEIEITEDE"
     gene            20307..>20653
                     /locus_tag="PL056_01735"
     CDS             20307..>20653
                     /locus_tag="PL056_01735"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HPM45766.1"
                     /translation="MSSHDFNEQEDNRIVSEINVTPLVDIMLTILIIFMLVSSFVAKE
                     AIEVDLPHAATGKETERKTLSVLVSKEGEYYLSGRKYETVADIGTELKKEKSENPQIQ
                     IVISADRLVYHGKV"
BASE COUNT         5796 a         4416 c         4034 g         6307 t
ORIGIN      
        1 aatccttcca taagtttcgt ccatttccat gtccccactt tcgcatacat ggtgtatttt
       61 atcccaccat cttcttcctg aatattagct atcgcccatt tctcacttaa agcaggggta
      121 tatgaaacaa agtttatgtt cctcggataa acgactcttt tgttgccgtc actgcaggaa
      181 taggtcagat aactccggaa agtgttttct tcagagtata tatgccggtt gtttacaatg
      241 tgaagcatgc accctttgtt ataagatccc gtttcaagaa gatccgggga caatgctctg
      301 tatttccttg tttcaggatc atattccatt gtctttgaat aagtctgata ttttttgtaa
      361 tcaagaacaa catttttcaa aaaaagaccc accttcccgt cacttatgtc aaaatgtttg
      421 atcgctcctg cggtggaatc tgtcgcccat ttaccgcctg ttgagattga acgggcggaa
      481 agaacaatat cacattcgtg tggacggtag ggaagtgaag gcaatccttc cagtgccgca
      541 tcccattcag cttgtgtctg tggctttaag tcagccatat cgcagggata cccgctctca
      601 cattctgctt caggtgcaac cgcataaagt gccgccgctg catctttcca aagattctcc
      661 acacattgcg gatctttcac tgtcggttta tcgcatttac ggcagaaatg aatgctcaaa
      721 tcatcattgt aatacgggaa cttcgcaacc tttagctctg gacactcctc cgccccctga
      781 taatcaacat ccgcatattc gacttcatca tcggtttcat ccgactccgc gtcttccgac
      841 tccgccaagg cttcgtcgga caaatcgtct gaatctttat cgggtcgggt catatcaatg
      901 tcattttcag tatcgttttc cgggttattg ccgttgcagg atgtgcatga ccagaacaga
      961 aataagagta atgcctgaaa aacaattatg ttgctgtttt tattcatttt tattttccac
     1021 caaaattcag ctcaggttgt attaaattat ctcaaggtgt caagaaaaaa attgaaagta
     1081 ttcagtaatg cttttttatg aaagatctgt tcttttcttt gaacagagtt cttgcgatgc
     1141 ggtctgcaac ggggatgacc atgctttcaa aagcacacag gattccaggc acttgagtca
     1201 ttgatccggt tgtatcccag ttgaggcagg ggcagtatgt tatgcatctt ccgtcaagtg
     1261 agcagtcgct gcactgcgtt cttggagagt ggttctctgt tgagacttca ctgcattttt
     1321 tccagtcaaa tccgctattt acatcgccaa gaacatagtt tgccgccctt tcaggtttcc
     1381 ctgcaaatct aacacagggg aaaattcttc cgtcttcacc caccgcacag atctttctac
     1441 cgacatcaca gaaatctccg ggcttgaaac cgcctttgat atgggcggca atgtgggcat
     1501 cgaacgctga gaaatagaag ctgtcaccat ttcgactttt tgcaagatac cactcttcaa
     1561 ggatcagaaa ctgttcaatt acctttgaaa gatcgtcttc ggtccaggga gctgtgtaat
     1621 cagccgccat gatatagctg ttgatattca gctcatccat gacatgtttt gcgttttcag
     1681 caatatgagc ggaagttaga ggggttatta caccgacaac tgcaagatgc tccgcccgtt
     1741 tcttaagtgc ggcaaggttt ctgacaacgc ttctgtatga atttccccca tcacgcatcg
     1801 gtctgttaag attatgactg aactcatctc catcaagtga aagttcaaca cggaacttat
     1861 atttctcaaa aaaatcaagc acttcttcat ttatcagagt tccgttcgtc gcaattgaaa
     1921 gatgcggtgc gaagcccttt ttatcagcaa ttttaaccac atattcagta atttctttta
     1981 tcagctcaaa ctgaagaagc ggttcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnaaaag ctgctcaaca caggtatatg ttgtgaggtc gcagtaatat ccttcttcac
     2161 aatctttatc ttccagacat tcaggaacag ctccaccatc tatctgatca aaatcattcg
     2221 tctcaatatc ggcagtatca ttatcaagaa aagaatcatc cggagggtct ggcagtccga
     2281 ttatttcatc gggagcactg ttatcattgt cagtaaccgt atcaaaatca ggaacctgca
     2341 ctccatcagt tctcatttcc tgctcaaaac aacccggaac cgttatgaaa aaaagaaatc
     2401 cgacaacaag aagagaaaac agttttatcg gaactgcaat tacaaaagat cttctgaaaa
     2461 atccttttac cggataaccg ggcttataat tttttaccgg cttaactgaa aaaccgctca
     2521 tttatctcct cctgaaaaat agttcgaagc tttagtatca aaaagatctc tattttattt
     2581 tacccatctt ctttaaaaca agtttctgaa ttgtttcaag aagaggaacg cactcatcaa
     2641 cattaagatg cgccgcattt tcacagaggg gtttttccgg tttaacgatt tttgcgtttt
     2701 tatctgtatc aaaactttca acatttacat ttatgtgaat tgagtcatca aacggttgac
     2761 cttcaaaatg ggcacggtgg cctgtttgag aagtatcaac atttaattga taaaaattcc
     2821 atagttcttt gatattactt ttaaactcaa gaatgtcctc aggatcttca aaatgtcttt
     2881 taacctgaca tgacatatca actcctacaa aaagtgatat atcaagcttt tcaagtcctc
     2941 ctcgacttcc ccccttttct ccggctacag catggtgaag cttacagccg cccatacttt
     3001 tcccgagcat tatcacctgc ggccttacac cggtaacttt ttccataagc ataacagtgt
     3061 tttttatatt ctcgctcaac atccatgttg ctgtcctcgg tcttccgtaa gcaccgatgc
     3121 aaagtgcttc aggggcaatt tcaggtgtca gatatttctc acagaaccat tctattccgt
     3181 catgaccgaa aagtccgccg tagcccccgt ttgcaacgac aagataccgg tgttcaggtt
     3241 taatttttac ttttgaagcc cagaaagagt tgatatccct gattttttcg caggcatacc
     3301 agtttgtccc ttttccacgg aatgatttta tatttacaaa agaaactccg cagttcattg
     3361 ttgaatcgca atgatcacat cgataatttg aatcgcagta tttaacgcac tcctcataat
     3421 tttcactttt taccggcttg catgcagtat aatttctccc gggaccttta aaagttttga
     3481 tcttttcaaa tttaaaatcg catgtacttg attttacaca cttaacacat tcaggatcag
     3541 ctgcacaata cttctggcaa cttacatgat tttcattaaa gaaaacactt tccagttcaa
     3601 caggtttatc tttgttttct gtactgtatg caaaaagaaa gatgaacagg atcagaaaaa
     3661 agaatgtaat attttttctc atcatccatt cctcctgtta aatgcgttca tcgattcttc
     3721 aaccccttca ccgatcatct tcaataaaat atctttccac ttggaactta ttgaattatt
     3781 tattaaattc aattcatcgt caagaaaatc cgataataca tatttttcaa gtggtaaatt
     3841 tttcaaaggg gctccgattc ctattctgat ccttgcaaaa ccgggtgtgc cgatctcccc
     3901 tgaaattgat cttatgcctc tgtgtcctcc gtcaccacca cctttcttta tcctgtgact
     3961 gccgaaattt atatcagtgt catcatgaat aacaataagt ttttcatggc tgacattaaa
     4021 agcgttcatt gcttttaaaa ctgaaatgcc tgagcggttc atgtatgttg aaggtttaag
     4081 caggataaca gttttccctg aaatatttcc aactgaaata tgaccttcca ttttcacgca
     4141 gtatgagaac ggagaaaaat taaattgttg agctatgctg tcggcaaata aaaatcctgc
     4201 gttatgtctg gtctttaaat atttattttc aggatttccc agtgcagcga caagaacgat
     4261 accggattta ttgataagca aagagtgaat tattcagcgg cagatttttc tgcagcaggt
     4321 ttttcctctt cgccttcttc agtggcggac tgacctcttc ccttgttgac aactattaca
     4381 ggagaatcct gatgaaaaac aggagttaca ccttgaggat agggaagttc tgatattttt
     4441 atacggtcgc cgatctcttt ttcagtgaca tcaacattta tttcagaagg aatatctgca
     4501 ggtctgcagg aaactttaac ttcttttaat acctggaaaa cttttccacc ggcgatctcg
     4561 cccagcgacc tgcccgacct tgtaacagga actgtaacaa ctatctgctg atcttccttt
     4621 acagcaatga aatcaacatg aattatgttt gttttgacag gatgtatctg catatcgtgg
     4681 gcaacagcaa gaattgattt tccttcatac tcaaggtcga aaatgatatt tttgccctga
     4741 gatgtatgaa gccctttgac gagttccttc cttgaaacgg ctccgctaat attgctttcc
     4801 ataaatttgc cgtaaatctc tacaggtact gaaccgtttg ttctcactgc cttaacactc
     4861 ttcttgccct ggtctctttt ttcaactatg agcttgaaag ttttcataat aacctccgat
     4921 atgacatttg gttggggcgg gaggattcga acccccggat gccaggacca aaacctggtg
     4981 tcttaccgct tgacgacgcc ccattaacct gttaataatt caattttcct gactgttgca
     5041 aatttatcac taaattcatc aagcgcattc tctcttgctt tgtcagtgcc gaatattgcg
     5101 atcagagaag aaccgcttcc tgacataaaa accttctcgg cgccactcag cattaagcga
     5161 tttctgacat cagcaagcag cggatattct tcaaaaacca catcctcaaa atcgttaaaa
     5221 atgctttttg tccagtcttt taaagaacca gtcccagttt gacggacgct atttatattt
     5281 acagaccccg attttgtcaa ttttttttta tcgaacaggg catatgcatt gcgtgtattt
     5341 acaataattt cagggtataa cagaagaaga tatttattta cccgctctag ttttaacggc
     5401 tccacaatct ccccttttcc acttataaaa gccggagagc cgtaaagaaa gaaaggaaca
     5461 tcgcttccga cagatgatgc aatttcgtta agccgtttta aagaaaggtc taaaccgaga
     5521 tatttgttta agaacataat aaatcctgcc gcgttactgc ttcctccccc aagaccggca
     5581 cctgtcggta tggatttttc aagtattatc ttgaagtcag gaacgggcat atttatataa
     5641 gatttaaaac tgttaaaagc tttaaaaatt atgttctttt ctttaggaat aacttcattg
     5701 atgccgcttg tttcaatttc aaattccgga ctttccttta tttttaaaac atcataaagg
     5761 tttacaggga tcatgagcga ttcaatgtag tgcatgccgg aaacttcgtc ctttccggtg
     5821 atcttaagaa aagcatttat ttttgcaggt gtctttgttt ctaaatacat ttaagcttct
     5881 ttctgagtgc tttctcaaca tcgggtgtga gaaactggga tagttctgtg tcgctttttg
     5941 atgctatttc cttaacaata cttgatgaaa gaaacgaata cttttcagaa gtcataaaaa
     6001 atattgattc aaattcgggc tctatgacac ggtttgtaag tgccatctgg aattcaaatt
     6061 caaaatcaga aactgccctg agacctctta taaccgtgaa aacattgatc tttttggcaa
     6121 aattcacaag caattcatta aatgatgtca cagttatttt cggattattc ttaaatattt
     6181 cagatatttg ttcttttctc tcttccgtag agaaaagagt tgttttgttc ttattctctc
     6241 cgactgcaat ataaatgtgg tcaaagatct tcagtgccct tttgacaata tcaagatggc
     6301 ctaaagtgaa agggtcaaaa ctgcccggat atatcgcttt tttatccatt ttcaatcctc
     6361 ccagctataa aagaacgaaa ggtgtgtgtc tccgaatttt ttatttctgt ttgtccatcc
     6421 atgtctgctc cattcagtca aactgcttcc ttcgacacat attacacata cttcgtctat
     6481 aatatctgaa ctcaaaattc tttcaagaag actggggatc ttttcaccca ttgaataagg
     6541 aggatcaatg tagatagcgt tgaatttcat tccggaaaaa caattcagat caatggaaaa
     6601 gacatctccg tttataactt tgacaagatc atccagaccc agttcttttg aatttgatct
     6661 tatctgttcc gccgctttag aatccacctc tactgcacat gcgcttttaa accccctgct
     6721 gacagattcg aaagcaaaag ctccggtccc ggaaaagatg tcaaggaatt ttctgtcttc
     6781 aaaatttata acagaaaaga gtgagttgaa aaatgccgcc ctgacttttt cacatgtggg
     6841 gcgcaaagat gtggacggaa cccggaattt tcttgctttt aaagatcctg aagaaattcg
     6901 aagaaacatt tttactggca ggccttaaga aatgcttcga cctgtgccct gtcaggagca
     6961 tccggagaat actgtatgta ctttttataa ttcacacacg cctgtttctg attttgaaga
     7021 taagcataag ctattccgag aaatttatat accggaccgt ttgtcggatt tattctggac
     7081 gcatttttgt attcatgtaa agcatcggta tattttttgt ctgcaagagc tttatgtcct
     7141 gccttcacat aactgtcaaa ggtgcctttt gaatcattct gaggacggac agtttctgct
     7201 tttttcacga ctttcggctg ttgtttccgt gcttctttct ttttaagtac tggaacctgt
     7261 ttttcaacta tttgtttttc atcctgtttc ttttcaacat tttcagccgg ctttacaatt
     7321 tcttttttat atatcggctc aatattctct tcatcgatat cctcaatatc ttcttcatcc
     7381 ggaacagcag gatttttttc ttctgtttcg tttatggatg caacattgtc ttcaccgctt
     7441 ttgtcatccg gaataagcgc gaaaatcaga acaacaatga aaagaacagc cacagcagct
     7501 gcacctatca atatttttct gtcaggcatc ttgaatccgg ccctgccttt ttcctctgct
     7561 tcagggtaga gctctttgat aatatttgct atctcctgct gctctctgtc cggcttggaa
     7621 gaaaagaatt cgtcattcaa agtaatcctt tcagtcggac ctcttccctt cttttcctga
     7681 tctttaccaa ccggctgttc agcttgttct ttctgaacag ggtcggcttc ctttttttcg
     7741 acaggttttt caggtgttgc aagctgttct gtcatagaaa caacagaaac attatcactg
     7801 gcacacattg gacatgtggt cctgcgatca ggaacttctg ctgaaaaacc acagttgtta
     7861 caaataaatt tcatagctca cccattcagt ggttttgctt ttattacagg tttcccttcc
     7921 ttttcttcaa ttgagaactg gacatccgaa catttatact tggccattat cgaggcgcgg
     7981 ttagttctaa cttttcctat aaacgatctt ttttcatttt ccgttactgt ttcaccgttc
     8041 ctttccttta ctttgcaata atccagccag agttgttcca tgggatccag cggttccggc
     8101 tcaggcgcaa tgctgtttgt tatcaaacct tctttgctgc ccacttccat tatctgggtt
     8161 ttttcattat catcgctgtc aaaatgtgat ggaggaggag ctttggcggt actttttacc
     8221 gtgcttctgt tttccgaaag ggcctgtacc ggttttctgg gagtgttctc tatgtcatct
     8281 acaccgaaag gatcaggtat ctgcgactca aagtctgatt tcttatttaa aaccgcatct
     8341 tccctcctta aaccggcatt cccgatcggg cctgatgatg catcggcatt ttcaaaatgt
     8401 ctcaaatatt ttgaaacaag ggtgtttaat gcagaaacag caatatctgc accgtgagaa
     8461 tattttttca ttaccagctt ttcaccgacc ttcataactg aaatatcagc cgctaggttt
     8521 ctaaagactt tgtctatcgt atgagttact atgaaggtga agaaaagcgt tatcattatt
     8581 ccggcaagtg tatacaacaa tgaatatagg actactttgg tataatcctc ccatcccggc
     8641 attgtggata ctaaagttat aaatacaacc ttttcgccac ccagcatatc atagggcata
     8701 aagtagctgt gcacatgggt ccatctgttg agttctttct gtccttgaga ctttataaga
     8761 tcttcaacgg cgttctttac tttttcgttc ccgcatgaat tttcaatttc gctccatttg
     8821 gaattcattg tttcagtaat gaatttgtca cccatgaaaa ctctgacagg cattggaaaa
     8881 tcattgacat tgaaagcaag agtgagtttt ttcacactga ctgccgcaaa aagcttctgg
     8941 ccttcaccgg gcacctccgt catcatagga acaaccccga gaagatacat cgtatcattt
     9001 acaaatattg atccgtcaga tgcataacct tctttcaatg ccctttcaac ggcagggatt
     9061 cctttttgtg aagacataag ttttatatca cccgcgacaa aatcaccgtt ttggtctacg
     9121 actacatatt cagtaagtga ttttatttcg gctatcctga caggtgcgtt ccttgatgct
     9181 ttctcaaatt tcaactgtct ggcgacattg aaagagttgg cgatgagaga atatgttgta
     9241 ttcttaactt tttcctctac cagcttaaca ctgtttttcg gaccctgctg aatatcgatt
     9301 ttagcaataa atgacctgaa aagagtaaaa tttaccgctg aaagagccga aattacaacg
     9361 gccattaaga aaaacacctt tagttttaaa ccgaggttgc tgaacatttt tatatctcca
     9421 aacttttaat gatcatatca gtctcccggc atcgatattg ttgttgaata tgatgtcgaa
     9481 tttatgctgt acacagtccc ttcctctatt ctgtatccta tattctgggc tgcatttgag
     9541 atcattacaa aatcttcctg atagacccat cttataccgt aatagaccct gacagtatat
     9601 gtccccggag ctatgtcggg gatttctatt ctgcttgaac cttcattcac cacaaaaaga
     9661 tatgtgctct gcagaaccct cacataaacc gtattccact ggaacaatgt atcaacgaga
     9721 gtatagtcgc cggaagaaga aaacacatgc gttattgaac cttttgcggg gaccgtcagt
     9781 tcgacttttt cgctgttttc cccttctttg agaatattta tctttcttgg aaaatcctcc
     9841 ctgttctcaa tcgtcagaac tccttcagtc ggaagcagaa tattgcttct gcttattccc
     9901 gcacctataa gttccagggt ttcattaaca tttttaatag tctgatatcc ttctattaca
     9961 aatgaatatt tctctctttc cagaggtttg gggacttcaa gaaccggaaa acgccagtaa
    10021 ccgtcaacta tttctgttgt tttgtgacct gccttttcaa tgcctttttt ataactcaca
    10081 agaagtttaa gcattcctgc ttctgcattg aaaggatgaa gtatcaggaa aacaatgacg
    10141 acaacaaaac aggttgttct tttcaaattg ttcacattac ctctttgatc tgcttttttt
    10201 tgaattttcc aattcatcta tctgctcttt tattgaactt aaaatctcat catcttctgt
    10261 aagcttttcc gcttctttca aaaccttaac tgcctgatcc ggtttatctg ccatagtata
    10321 tgcctgggca agccctgtga ggcatgcaag attttcactg tctgctgaaa gtattttatt
    10381 gaaaagagtt attgcttcag agaacattcc cattgaaagg tgactcaacg ccatatcata
    10441 aaggtcttcc gttcctccgc cgtattcaga tgaattttca agaacatttt tcatctcttt
    10501 cattgtgtcg gaaaggttat cctcctttct taccggaaga atgtctttta tgccgcttaa
    10561 aaaacgggaa tcgggatagt tgataagaag ttcctgaaga agctgggttg cacttgcaaa
    10621 atcttcaatg cttatataga attcaagttc atcaagctgt acctgaggcg gttcctgaag
    10681 ttcagacaga atatccatcg tcatgttttc aaattcagat ttttcagtgc cgctctgttc
    10741 atcggcaaca tttgtcaatt caaaaggttc tgagcgttct tcctgctcta taagttcaac
    10801 cagttcgtct tcgtcttcgt tttcagaaac agcactgacg gtctctttct gcggaagatc
    10861 gggtatcccg tcaaaaatat cattgttctg gatgtcactg tccagatccc tcatgtgttc
    10921 aacgatcaat ccttgtcttt tgctgatcct cggatcgtcc gtaccgacta tattttctgc
    10981 atcgactaga agttcttttg cctcctgata tgcccccgta ctaatcttaa gatctatgag
    11041 cgacattata agatcacccg ccgtttctat ttctcccgac tcgatatttg cctggataag
    11101 aacatctatg gcttcaggag aggtctgcca ttctttacat gactggatct tgtctattgc
    11161 cttgttgaaa agtctgtaac ttaaaaaaac tccggcttcc tttattgcgg tctcgatatc
    11221 tatgacaggg gcggactctt cagtttctgt aacctgatgt tcatcttttt cattctctgt
    11281 ttcgtcagca tctatgtcta tgtcgagaac gagatctata tgttcatctt cttcgcccat
    11341 attcatcatg ctggaatcaa ttttatcaga aatgaggtcc ctgagcttgc cctcttcctt
    11401 gagtagggaa agtgcctctc tgtcgtttgg atcaatatca agaattttcc tgtatatcct
    11461 gtctctttca ggaatgtcct gtctctgagc acaggcaaca gcgatctgtt tatatgtcga
    11521 tatgagtttt ttggtttcac cgagtttttc aaaaacaaat gccagttttt caagaaattc
    11581 tatcgaatcc ggtgtgtcga ctataagttt ctgaagaact gccagggcat ttgaataact
    11641 tccgctctta agataaagat tgaccaggtc tcttcctatc gcactttcac tgttcgttgc
    11701 tgtatagaga agtttcagga caccggcata ttcattcact ttatcggtgc cttttaaaga
    11761 ctcggcgacc ggaagaagaa gtttttcagc ttcgtgatac attttttcgg caacaagcat
    11821 gttgccgagc ttgattgaaa agttaatgtt gccggggaac atttcgatca tttttcttaa
    11881 aatgtttatc gcggcaagtt tgttcccgat ctttgcatag taattgacag cagtctgata
    11941 tctcattgtc gcatctttga tcaaaccgct ctgtctgtcg tagtcggcaa gaagaacatt
    12001 tatcttgcca tgctcggaat ttatcatcaa tgcctgtttg agaactgcct ttgccttgtt
    12061 aagaaaacct ttcttttcaa aaagtatcgc cgccttttca taaagctcga tagctttgtc
    12121 ttcgttcccc gccctcgcat aaagctccgc aaggcggaca taggccctgt catcatcagg
    12181 tcttttcttt ataagatctt cgaactgctg aatttctgtg tcgtttttct ttcctttaag
    12241 cagaaaccgt ccgggatttg tatttgccaa tttgattacc tttaatttta ctttttaaca
    12301 cgctcaatgt aagtgaattc ttcagtgctg atccttatca catctccggt tgaaatgaaa
    12361 agcggaacca tcacctgtcc tcctgtaaca agctttgccg gcttgtatga agttgtaaca
    12421 gtgtctcctt tgaagccggg gtctgtttca acgatctcga gctcaatgaa ctgaggcaga
    12481 tcaagtgata tcggcgagcc gttatagaaa agaaccgtaa ctttttcgtt ttccttcata
    12541 tatgggatgg catcttcaat tactttctcg tctattgata tctgttcgaa tgattcgctg
    12601 tccatgaaat agtagtggcc tccgtcagta tacaggtatt ccataacctt ttcctcaaga
    12661 tcaggtttgt caaccctttc accggagaga aatgttcttt ccgcgataga acctgacagc
    12721 acatttctaa gttttgcttt aattacagct ctccttttct gctgtatatg ctgaagttca
    12781 acgatctgat aaggaattcc atcaatttct attctcagcc cctttctgaa ttctgaagtc
    12841 tgaattgcca ttttcaatct cctttatgtt aaaaaaacaa cccctcaacg aaaaagcacc
    12901 aaatactaat gttgaggcgt gtttttgtca agttttagaa cttctgttct cataattttt
    12961 taaaattaaa tatagtgcct ctactgcaga agcataagta tatatcccga gcccgcagat
    13021 aaaagcggaa gcgatgtcgc tcagaaagga acggtgcctg aaatcctctc tgccgaatat
    13081 gtttgtcagg tgggcttcaa caaatggaat tccgctgcaa agtgcggcat cccttataag
    13141 aacgcttgta tgtgtgtatg ctcctggatt cagaaccatt ccgtcacatt tgcaccctgc
    13201 tatcagccct gcgagctctc cttcaagata actgttcatg aattcgatac ttaccgaaag
    13261 ctgtcccgct ttttcaaggg ttgttcttac aagatcatcc cttgtaaaag aaccgtagac
    13321 agagggctct cttgttccga ctttatcaag aaggggacct tcaataatgc agatcttcat
    13381 atgaaatcct cgtcgtcatt tatttcattc caccttatct gtgcccacag cgaaagaaga
    13441 tatgccgcat cgggtcttga ttgagccgct tttgatgcgc cgtttatcac tttcattaca
    13501 tctgatcctt tttcagatgc gatctcgcgt gacattgatt ccgcctgcat ccagttcttc
    13561 tgtttaaggg ctataagcac cattgtttca gtgatcattc cacacctccc cggaaaacaa
    13621 tttgaagcta acagattgat ttctgtgagt caagaaaagt tgcctttaaa atgaacttct
    13681 gatatttaac cgcaagttgc tgttttttat aaggagaatc aaatgccagt gatttcaagt
    13741 gctatcatca gcgtttttga caaaagaggg cttacggctt tcgtttcaga gcttcataaa
    13801 atgggcgtca gcatctattc aacaggcgga acacataaaa tgataagctc ggaaggaatc
    13861 agtgccgtaa aaatcgaaga ttacaccggt ttcccggaaa tgatggacgg ccgtgtaaag
    13921 acaatgcatc ccaagatcgc aggagggatc cttgcctgcc gtaatgatga aaaacacatg
    13981 aagtcggctc gtgaccacgg aataaaaatg ttcgatatgg tcgtagtaaa tctctatcct
    14041 tttaaaaaca ccgttgaaaa aggcggggaa atggctgata ttattgaaaa tattgatatc
    14101 ggcggcccca ctttgataag aagtgccgca aaaaatttta aagatgtcgt tgtgataact
    14161 tcgcacgaag attatgcggc ggtgatcgaa gaaatgaaaa acaataacgg agctgtttcg
    14221 attgagacaa aattccggct cgcaacaaaa gcgttctccc atactgcact ttatgacggc
    14281 tacatcagct cttatttttc aaagatagac cataaaggcg agatactcaa tgaagatccg
    14341 gggataataa cccttcagtt tacgaaaaaa gagcatctga gatacggtga aaacccccat
    14401 cagaaagctt cgttctatgt tgacaattca gggatcacag gaacagtcgc ctcggcaaga
    14461 cagcttcatg gcaaacaact ttctttcaat aattacatgg acttggaaag tgcaaaaaac
    14521 atcgttgcag attttgatga gccggctgtc gcaattgtga aacatacaaa tccctgcgga
    14581 gcggcgatcg gaaaaacttt aaaggcggcg tatgaatcgg cacttgcctg cgaccctgtc
    14641 agtgctttcg gttcgataat tgctgtcaat aaagagcttg atgctgaaac cgcaatggta
    14701 atgaaagagc ttttcgttga agctgtaata gcaccttcct ttagcgaaga agcccttgaa
    14761 atacttaaga aaaagaagaa catacgcctt atagagacag ggacttttgc aaaaaggcct
    14821 gatatggact gggaattgaa aaaagtttcc ggagggcttc ttattgaaga tgccgacaga
    14881 aggataataa ctgaaaatga tcttcagttc gttactgaga aaaaaccttc cgctgaagag
    14941 ataaaagagc tcctttttgc acagaatctg gtgaaacatg taaaaagcaa tgccattctt
    15001 ctttcaaaga acggtgccac agttggtata ggtgcgggcc agatgtcaag gatcgacgct
    15061 cttgaaattg ctatcaaaaa ggccaattta ccagtgaagg gatgtgtgct ggcaagcgat
    15121 gcgttctttc cgttcagaga cagcgtagac atggcggccc gtcacggtat caaagcgatt
    15181 attcagccgg gcggttctgt aaaggatgaa gattcaatta ttgcatgcaa tgaacacggc
    15241 atatcaatgg tcttcacagg tataagaagt ttcagacatc tttgatatta acaaacagga
    15301 gtttcaatga aaacttattc aatcaatctc catctacctg aaaacattat tgaatctatt
    15361 gaggttaagc agcgtacgac atttcttgat ctggcaataa aatatcaaaa gagctttgac
    15421 tgcaaaatag tcggtgccgt ttttaacaac gaactctgcg aacttaaaaa agaagtgtct
    15481 gataacggga atcttgaact gataacgctc agggacaagg atggttatct tttctatctc
    15541 agaagtctgt tcttcattct cataaaggcc gcgtccgaga tgttccctca tgcgacactg
    15601 cacattgaat acagcatcgg aaacggatac tactgctggt ttgaaggact cgagtggata
    15661 gagccgggag agatagaaaa acttgaagca aggatgaaag agatcgtaaa agcggatatc
    15721 cagttctcaa gaagaagggt tccgacttgg agtgcgatcg aagagttcag gaagaatggt
    15781 ttgatggata aagttatgct ttttaaaata agggaagaaa aatgcacaag tgtatatatg
    15841 cttgaatcaa ctattggata tttttcaggt tatcttgttc cttcaacatc atactgcgac
    15901 cgtttttcga taagtttcta taataagggg atcgttattg tcgcacctga tccttcaaat
    15961 ccgtccgttc tgcctgcgtt caaggacagt cctaaatatt tcaacatcat caaagaacat
    16021 gcggaatggc tggacatcct tgaaatggac aactgtgcta agctcaacga cctgataagg
    16081 gaaggaaaaa gccgtgagct gatacttctt agcgaggccc ttcacgaaaa aaaacttgca
    16141 aagatagctg atgaagtcta ccgcaggaac aaagttataa agatcgtttc gatcgcagga
    16201 ccaagctcca gcggaaaaac gacaactgca aaccggcttg cggttcagct cagggtcaac
    16261 gggatcaaac cgttcctcat ttcacttgac gactacttca ttgacaggga cagaactcct
    16321 gttgacaaga acggaatgcg tgatttcgag tcgatatcgg caataaatgt aaagctcttt
    16381 aatgaaaatc ttgatgacct tttaaaagga catgaagttg aacttccgac atacgatttc
    16441 aagacgggca gaagcatgaa gagcggaaga aaactgacac ttcctgaaaa cggtgtagtt
    16501 gttgtcgaag ggatacacgg gcttaaccct gacctctatt tcaatgttcc cgaccagaaa
    16561 atatacagga tctatgtaag tgctcttacg gccttaaata ttgacaatca caatagaatt
    16621 tcgacaacgg atgaaaggct tttaagaaga atggttcgcg acaacaaata ccgcggacac
    16681 acaccggttg aaaccataag aaggtggccg gcagtgagaa aaggtgaaga aaagaacatc
    16741 ttcccgtatc aggaaaatgc cgacataatg ttcaattcaa gcttagttta tgaattcagt
    16801 gtcttaaaaa aatatgcaca gccgctcctt gagaatatcg aaaaaagcac accggaatac
    16861 agagaggtca aacggctttt gaaatttctc tcattctttg aggaaatgga tgattcgatt
    16921 atccccccca attcaatatt acgggaattt gtcggcggaa gcgtgttcat ttaaatttag
    16981 ttgtggacaa aaatcgcgta tctgatatat ttcactgttt ttgttttccg gggtttaaaa
    17041 gggagccggc atgacatttt taaggacaat aatattctca gcactttttt ctgcaccgtt
    17101 attgatgttt cctgcagatg aaccggcaga taaaccggta aaggaggaaa caccggttga
    17161 aactgttcag aaagcagaaa agatcgaagt aaaaggaact ctccttgagc ggggaacacg
    17221 cgcacctgtt tccagaaaag tattttatat cattgaaaag gacaataaga aaataagaac
    17281 ggataaagaa ggcggcttca gtttttcact tgccgaagga acatacactc tctcttttcc
    17341 gatagtcggc tatgaaaagt ttgaaacgaa aatagaagtt aaaaaaggtg agaaacttga
    17401 actgaccata cggctcgaac cgcttgtgat aaatccttac aagatcgttg tcaccggtga
    17461 aaagaacaaa ggggaggtct cggttcaaag gatcacgatc gaagaggcat caaaaattcc
    17521 cggcacgaac cgggatgttc tcaaagctgt cacaagtctg ccgggagtca caaatatttc
    17581 agttttcaac gggataggaa gcggtcttgt cgtcagaggc agctcggcag gtgattcgag
    17641 cacggtggtg aacgagcacg gcattccgct gatgtaccat ttcggaggtc ttgaccctgt
    17701 cattgagccg gaacttgtcg aatctcttga cttttttgcc ggcggttttt caagcgagta
    17761 tttcaacact atgggcgggg tggtccagat aaatctccgc gatccgagga aggacaggtg
    17821 gggtggatat gtcaacctga gtcttctcaa tgtttctttc atggtggaag gaccgatttc
    17881 caaaaaggat tatcttgccg tcgcctttaa aagagggatg ctcgatgcat atgtaaagat
    17941 cgtggaagag cttggattct ttaataattc agtttctttt tcgacttacc ccttctatta
    18001 tgacgcaatg attctttatg ttcatgaatt caataaaagg aaccggctca aaatgtatac
    18061 tgtcggctca tgggatgagg tccggctcgg atttgacaat cagacatcta tccagaagat
    18121 gagcaacact gcaaaaacca agactcagtt cgttcagcat acgacggaat ggcatttccg
    18181 cgaaaatgat ttcaaaagtc ttttttcacc aatggccgag gttgtctggg tcgatataaa
    18241 tgcaggggat gcggcatatt tcaagaactt tttctttcag ctagaactga gcgaaaaaat
    18301 ggaagtgaaa cttaatgata tccacaagtt gagatttgga gccaaattct attacggttt
    18361 ctacacgatg gatgtcaacc tttttgcgat gcccaaggaa ggtgaagtcg catacaatcc
    18421 tctgagcaac gagatcgagg atcactctga aggccactac tggtttccgg ggttttattt
    18481 catggatgag ataacctata acgggctgac tgttgttccg gggataaatt tcatctatga
    18541 cgagcacaac acacagatga tgttcgaccc ccggctgaca attaaataca aaattaacga
    18601 tcaatggctt ctcaaaactt ccggcggact ttattcacaa ctcccttcga atgacgagtc
    18661 ttatgaaccg tggggaacac ctggactgag acccgaaaaa gcactccatg cgatagtcgg
    18721 tgttgagtat aaccctctgg atttccttga gtttgatctt cagggatact ataagcactt
    18781 ctttgatctt gtgagaaggg atgactcttt tgacctttca tctttttcaa acggcggcat
    18841 aggttatgcc tacggtgtcg aatttctgat caggcacaaa atgaccgaaa agttcttcgg
    18901 atggatatca tacagcttct gcatgtctga aagaaaggat tctcctgaca gcgactggag
    18961 accgttcgat gttgatgtaa accacaacct gatcgcagtt gcgagttata aactcaataa
    19021 atactggcag ttcggtgcaa gattcaagct tgcaagcgga gcaccttatt ccaatctgat
    19081 gttcactgac tactcatttg atgccgataa caatttcaat attccgcaat acagaggtga
    19141 tgtgaatgat tcaagactgc caataactca tcaacttgat ttaagaattg ataaatactg
    19201 gctttttgat aactggatat tctcaacata ccttgacatt cagaatgtct acatggcaaa
    19261 gaatgtcctg ggtgtaagtt accggaacga ctacactgaa aaagtttaca catacggatt
    19321 cccgataatg gtctttctcg ggttcaaggg ggatttctga tgaggaagta tttaatatta
    19381 ctggttttta ttactgccgt ttcgtgtgag ccgaatccgg aagttaagcc gggctacatt
    19441 gtcggagaca tccgtcctct agggatgaaa gccgagccgg tagaagctgt tcccggtgac
    19501 atgataactc tttccatgct gatcggaggc agaacatttt cccagggaag cgacctgaaa
    19561 atttcgtggc tcgggcttga agagctgacg ataccctata caaaaccgtt aatatttcct
    19621 gttcccgaaa atcttgaaga gctcatgcct gccgattctg acaacccgca agcatcttta
    19681 tttattgaca attttaacaa gaacggtttt gcagaccttc ccgttacggc atcttttgaa
    19741 attcctgttc aggggggacc cgaaacaagg acgctcaccg taacaaaaac agtaagaata
    19801 tacaaggagc cgcctgaaga cggtataagg aaaaatccgg ttatcaacca tgttcttgcc
    19861 tcttatgaaa tttatgggtc cgtaaaaagc tttgagattg aagacggtgg atttatttcc
    19921 ttttcgatat ctcaggttcc tgaaacaatt attttcagat ctatccctct ttcacaggag
    19981 gaaaccggct gggaccggct gaattttgca tggttcttta caagtgatac cggagagtta
    20041 cttgaaaacg acatggaagt agagcttgat agaaaaaaat atggtgatgc ggttcctgaa
    20101 gatcagaaag ctactcagaa cagggaatat ttcgcactga gtttcaaacc tgttctcgaa
    20161 gaaattgagg aaagcaagat aaagctgccg gtaaaattca atttttatca cattatcagg
    20221 gacaaggcga tcgatgcaca aagcagtgcc gattacaggt ggggacagga ttttatgtgg
    20281 tttgaaattg agatcacgga ggatgaatga gcagtcacga tttcaatgaa caggaagaca
    20341 acaggatcgt ttcagaaata aatgtaaccc ctcttgtcga tataatgcta actattctga
    20401 taattttcat gcttgtcagc tcttttgtcg ccaaagaggc gatcgaggtt gaccttcctc
    20461 atgccgcgac aggaaaggaa actgaaagaa aaacactttc ggtccttgtg tccaaggaag
    20521 gagaatatta tctttcaggg agaaaatatg aaactgttgc agacatagga actgagctca
    20581 aaaaagaaaa aagcgaaaat ccgcagatac agatcgtgat aagcgcagat cgtcttgttt
    20641 atcacggcaa agt
//
LOCUS       DAOOYB010000015        19863 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_2604_length_19863_cov_10.900697, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000015 DAOOYB010000000
VERSION     DAOOYB010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 19863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19863
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2604_length_19863_cov_10.900697"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..699
                     /locus_tag="PL056_01740"
     CDS             94..699
                     /locus_tag="PL056_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45767.1"
                     /translation="MTKLSKSFVFYMKNFETMQALNELDEENEDEINSLFEGSGFKNA
                     SDMVDSVTEVANQTAASILLKIGMEVDEKYSFKMISRFNKIKENWFVENWMYKKRPET
                     KNGYVLSIQICSKGDTTEGIGFLSCSIYDSRKKDTALLEKIFSENIKEEYDDIFGKNE
                     WLILETVILEEDSEEKIIEDFMVKLSEILTQDNLTKLFAKP"
     gene            797..1057
                     /locus_tag="PL056_01745"
     CDS             797..1057
                     /locus_tag="PL056_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HPM45768.1"
                     /translation="MPELSRFYGIVIAMFYDEHNPPHFHAVYGKHKVAVRLDNFAVIS
                     GKFPPRALGLVIEWAMLHKEELEKAWEKLIAQEPLFKINPLK"
     gene            1066..1323
                     /locus_tag="PL056_01750"
     CDS             1066..1323
                     /locus_tag="PL056_01750"
                     /inference="COORDINATES: protein motif:HMM:NF021873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HPM45769.1"
                     /translation="MHFDIKKAVYLGGYKIKLFFRDKKIGIVDFENYPPRKGVFEKFK
                     EIEFFKQFYIDPDFNVLAWPGGIDIAPETIYEIATNTKQSS"
     gene            complement(1434..4238)
                     /gene="ppdK"
                     /locus_tag="PL056_01755"
     CDS             complement(1434..4238)
                     /gene="ppdK"
                     /locus_tag="PL056_01755"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HPM45770.1"
                     /translation="MVKKFVYFFGPEGSEGNATMKNILGGKGANLAEMCNLKIPVPAG
                     FTISTEMCTVYYENNRNYPPELREQVMENLKKVEDVMGKKFGDKKNPLLLSVRSGARV
                     SMPGMMDTVLNLGLNETTLKGLIKTKADERFAWDSYRRFIQMYGNVVMEVKGENFEHA
                     IEGLKKKYKLVNDLDMTADHLKELVAEYKKIVKKISGKDFPTDPMEQLWGAVEAVFGS
                     WMNPRACYYRKMEGYSDKWGTAVNIQAMVFGNMGADCATGVAFTRNPATGEKVFFGEY
                     LINAQGEDVVAGIRTPQQVTLSGSRNWAESNSVKEKERKSKYPSLEEYMPEVYEELCD
                     IYHRLEAHYKNMQDIEFTIENKKLWMLQTRNGKRTAAAAVKIAVDMVNEGMITKDEAL
                     LRVEPSQVDQLLHPQFVKAEKAKAKAIAKGLPASPGAATGMVVFNAEDAVEWKKQKKG
                     TILVRIETSPEDIGGMDAANGILTARGGMTSHAAVVARGMGKCCVSGAGAIVIDYKAQ
                     QFTVKDIVVKQGDWISIDGTSGEVFLGKIATEEPKFEGEFETLMKWADERRVLKIRTN
                     ADTPKDSKVAREFGAEGIGLCRTEHMFFEGDRIKAVREMILADDEEGRRKALKKLIKH
                     QRDDFYGIFKEMEGLPVTIRLLDPPLHEFLPHEPKQIKEMAKEMKVTEAKIISKVEEL
                     HEFNPMLGHRGCRLGNTYPEISEMQARAIMEAACDLTKEGVKVFPEIMVPLVGKVEEY
                     RLQKEIIISTVEQVFKEKKITVEYMVGTMIEVPRGALTADEIAEEAEFFSFGTNDLTQ
                     MTFGYSRDDSGKFLKEYIEKKILTIDPFQSIDQEGVGQLVEIACKRGKKTRPNIKLGV
                     CGEHGGDPASIEFFHRCGLNYVSCSPYRVPIARLAAARAAINNDVVREIKKTMEEAYG
                     TIESGIKSLYKEIEKRLKKN"
     gene            complement(4250..6307)
                     /gene="glyS"
                     /locus_tag="PL056_01760"
     CDS             complement(4250..6307)
                     /gene="glyS"
                     /locus_tag="PL056_01760"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HPM45771.1"
                     /translation="MAHFIFEIGFEEFPAGFIVPAAEQMRAFLISELEKARLANAKCQ
                     MYSTCSRIAAVITELPEKQPDRKELVTGAPKKVSLTPEGTLSKAGESFLAKNGITEYF
                     FENSDKGEVIAGWKEEKGMEIQEVIIPIVKNGIKALSFKKSMRWGEHKFPFARPIRWI
                     FLSINGKPVADKFEDVDFSLTSFGHRFLANGPLTVSAQDYEKVLKGHFVIADRDERKK
                     MINESVNAIADKNQLIANIEENLLDEVTDMVEYPHVIVGTIPEKYSHLPAELITKVLK
                     KDQRYFVLCEQGEKTVVKKFASVLNNIPTDDSVVVKGNEKVVSARLADAAFYFTDDLE
                     KDFHGLKEKLREVLFQKDLGTYHDKIVRIGKIAEFAAQKFFKADKSQIEKIKTASEMI
                     KNDLVTGVVFEFPDLQGIMGRYYAEHAGFDPEISKAVYEHYLPVNAGDLLPSTLTGTV
                     LSIADKVDTITGGFMAGMKPTGSKDKFAIRRNAISLLTLAVENEKASINLRELFEFSG
                     RLILEHNGKLKLDVNEIMEFMTARYNAVLNFETPVIQASTATGADIPAVVKKRAETIN
                     RLLKDNEITGLAQLYKRGCNILKKQENIFGEPVESLFELAEEKNLFKAADTLCQEIVN
                     MSDNLEIALKIITIKPVLDSFFDAVFVMAEDENIRMNRMRLINKVTTLVIKNIGDISY
                     LNI"
     gene            complement(6307..7164)
                     /locus_tag="PL056_01765"
     CDS             complement(6307..7164)
                     /locus_tag="PL056_01765"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HPM45772.1"
                     /translation="MTFQEMIFRLQRFWTEKGCILVQPWDIEMGAGTFHPATFFYALN
                     PANISSVYVQPSRRPTDGRYGESPNRMQRFYQLQVLVKPSPENAQQLYLDSLKVIGID
                     PKDHDIRFVEDDWESPTLGAWGLGWEVWLDGMEISQFTYFQQVGGIDLHPVPVEYTYG
                     LERICMYLQDVDNVYDLKWNDRITYGDIHKMDEVEFSHFNFKEAPVEDFFHSFDKYEK
                     ISRELLEKSLPRPAYEFCIKASHAFNILDARSAISVTERQRYITRIRDLACAIAGKYA
                     ESFTNVGGK"
     gene            7330..7821
                     /locus_tag="PL056_01770"
     CDS             7330..7821
                     /locus_tag="PL056_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45773.1"
                     /translation="MKKGVVFQESQRFTQWWLWLVVIGMDLMYLFAVGKHLFSQIQSG
                     KEPQINSQIIIAVTIVFLITVLFLLLRLDTIIDDAGVHVKFFPFHLSFRHYGWETIAN
                     CFVRKYKPLLEYGGWGIRGTGKNKALNVKGNMGIQIEFHDGKRLLIGTSKFEEVSKFL
                     TGR"
     gene            complement(7831..8949)
                     /locus_tag="PL056_01775"
     CDS             complement(7831..8949)
                     /locus_tag="PL056_01775"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HPM45774.1"
                     /translation="MKRLFFLIFLLTSVISAGEIDIEFSAAIQAGNCVAASEALKKGA
                     DINYVDEEWPLFITAVTSNDIQMVRFFLNNSVNTELKGPDGKTALMHALSLKNAEITA
                     LLVKSGSDLKAKDPSNKNIMMYAAEGNSPELLKLLLDMGFDRKVRSKADKTALDYAVD
                     ARAAECSRILSKLDTLPMDFIEAVEKGDVNLAKKLVGDGADINTKDRNNKAAILVAIE
                     NGHENILKFLLEKGADPNGSYFKNKNATLFVFAMHNGRFNEAVHLLRAGATANFNHRY
                     KDGKTALMLAITNNNPSLINLLVDKKQNIDTIDSFGNTALMFAAEANLFSVVSKLLEK
                     GADPTIRQVEGKTASDIAKAKGHLQISNLLAQAEKKWI"
     gene            complement(8978..10120)
                     /locus_tag="PL056_01780"
     CDS             complement(8978..10120)
                     /locus_tag="PL056_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45775.1"
                     /translation="MKKSLIAIISILMFVFLSCGESSTGTNDYDKITTKLIKAVEGGT
                     IESADGLAKLDIPADALESDTNITIQKRDPSKYPDKEYLGSNIYDFGPDGLQFKKNAT
                     LTLKTTKNKVAGQIITVAVYKEDKWVYAGSGFEQSGDPIMSGDPIMSGDPIMSGDPIM
                     SEVGHFSEFSVMIFDEKDLTDEDQTNDEAVRPDDETGDTEVQDIEDTDDETIDDIIPD
                     EDVTPKNPDDLLGEWDFPNATHVSVMKNEEAGSGYMLDISINDYTEFFMAHGDENSFV
                     GYTFTGEFSIWVEQVEKTGNMTAVFTLHTDDKLDIELNGDDPMGKRTLTGGTRPEPFC
                     GDYMVNNEEICDGNSIECKDITDRSYTVGWAYCNSDCTGWDETQCE"
     gene            10239..11594
                     /locus_tag="PL056_01785"
     CDS             10239..11594
                     /locus_tag="PL056_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HPM45776.1"
                     /translation="MTEMFVFIEKGVSELGALAWGPPMLVFLVGTGIYLTILLKGLQF
                     SRLFYSLKLIFVKIGDEEKGHGDISNFQALMTALSATVGTGNIAGVATAIAAGGPGAL
                     FWMWMTGLVGMATKYAEAVLAVKYREKDKHGAMCGGPMYYISKGLGQKWLGTLFAVFA
                     AVATFGIGNMVQSNSVAAAMESTFGVSSWITGAVLMVFSAFVLLGGIKSIGKVTAVLV
                     PVMIVFYTGYAMFIIFSNMSQIPSAFAIIFEYAFTPVAATGGFLGSTVMLTIRMGVAR
                     GVFSNESGLGSAPIAAAAAKTNHSVHQALISMTQTFIDTLVVCTMTGLVIIMSGLWKD
                     GKTGSDLTASAFSWGIAGGNIVVTVGLILFAYSTLLGWCYYGEKSIEYLFGERSVKPY
                     RTVFVILILVGAISKLDFVWSLSDTFNGLMAVPNLIALLILSPVVVSETKDYFAKIRK
                     P"
     gene            11604..12911
                     /locus_tag="PL056_01790"
     CDS             11604..12911
                     /locus_tag="PL056_01790"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPM45777.1"
                     /translation="MKNVRTLILGAGLAGLSAAYHLKGKDFLVIEKSDHPGGLCTTEH
                     KDGFSFDQTGHWLHMKDERTKALFNTLFNDEFVEIVRKTFVFSHNVFTQYPFQSNTYG
                     LPSDVIKECVLGFIKAKYENDKSKAGDNFYEWCMAYLGEGISKHFMIPYNTKIYTVHP
                     KDYASHWCDYYVPKPTLDQVIEGAVTSPEQKNVGYNATFKYPENGGIGELPKRLFDKC
                     EKEKFLFNVHPVEINSDEKTTLLSNGEIIKYENLINSIPLKDFLKLQTGSAGSWSNEI
                     SDRMKIASVSYLNVAFNRLPGHTGHWFYIPEEKFMPYRIGSFSNIYSKLAPDGTGSAY
                     IEYTHQGDFSEIELLKQKSVELMLEMKMIKSINDIKFMDYRKIENGYVIYHKEYFEDL
                     KRIEKWCAENSIKLAGRYGKWTYAAMEDAILDGMSAVSGLYNI"
     gene            complement(12889..13839)
                     /locus_tag="PL056_01795"
     CDS             complement(12889..13839)
                     /locus_tag="PL056_01795"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HPM45778.1"
                     /translation="MRIIFQVMITVVFTFVVINGCAEKKDPAIYDNYPDESETEKNDE
                     GGCGVIPDENIQKPDKTQSDPDEIQEENVTYDEEFLPDSDEATVAETDIETGDDIQDS
                     DIADDSDAIADGCPSDMVENGTFCIDKYEASKKDATDISHGSDESVAVSKKSVLPWMV
                     NPMTAADYQKFKTACTAAGKRLCRDDEWIFSCEGTEKRKYSWGGTYNREICNTVDAFC
                     DEHCETNSIAPESCLMSENCGYSYYCFNVVKTGDFLNCINHSGAFDINGNVWEITDTG
                     SAYKIRGGAFNCGSPSSRLECGYNATWSELFAGFRCCKDR"
     gene            complement(13906..14502)
                     /locus_tag="PL056_01800"
     CDS             complement(13906..14502)
                     /locus_tag="PL056_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45779.1"
                     /translation="MLSKIEEFGKNKGYFQYKKIGLLNSTPVQNTKTDVIDFDDTVIR
                     VCRDHDIGDRPKSCDALKFICEQNRLDFLELKSVVKIIENAHRTPPKPIEESLEKIRV
                     AIPKKIAGSNLIMNYIINHKELSLSPEELSEYAACSKNFYLIVDNDISQDGLSSFYFN
                     MVFASAFRQIENDNPIIVKGLMEIDEEYRSAGRQLPSG"
     gene            complement(14492..15769)
                     /locus_tag="PL056_01805"
     CDS             complement(14492..15769)
                     /locus_tag="PL056_01805"
                     /inference="COORDINATES: protein motif:HMM:NF024573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPM45780.1"
                     /translation="MKLSLRNIGIINQADINLDGLTVIAGDNDTGKSFAGRTLFSIIK
                     ASNSSGIVFTDELERNIKDIVQEIYIEIRKNLDKEGNRILFDILNPGSFIKELRSAVV
                     AENVFKIEDIIQKRISLLKTSSINKPLLNILLPKLEDLRTVLGIKDTEDKNKIQSKTY
                     KEILQKEFNYNIRNMHSKDNGKISFSEGSNELLNIQLSNGNIEVEYSDNLLVPTATLI
                     ETPFVLQMEGLFGNYNKSLPVHITDLFEKLTGSMGYFDDDTDIFLKKIESTIQGDWDY
                     DTEKDDFIFSKEISGEKHSFDSLNTATGIKSFGIISLLKKAGVLEKRCVLIIDEPEVH
                     LHPKWQIEYAKIITELVKEGVIVLVTTHSPYMVQAFSVFSKEADIKSDFYLSHKNDNG
                     TAEIENVSEDINKIFLQLSKPLNDIVWGKKYAL"
     gene            complement(15779..19441)
                     /locus_tag="PL056_01810"
     CDS             complement(15779..19441)
                     /locus_tag="PL056_01810"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45781.1"
                     /translation="MESVSRNEKIVHLLKKCGVSIRPENEFESIYAHTLVKYSVECKN
                     KNFADFFAKKEIWEAFRMKFSSNYDEFEDRINTYLNIGDDRFMKIRSDGYRNVDDLKI
                     EIERFSKIFDETIKEAMKPSEVKLQDSQDLISKKLDSVMDLLQVKNNIDISGEFLKKS
                     KELLDQKEYDKARIWLEEFNKTHLDSNEKKWKYFNNLGVAYIGVGNQEKAADYLIEAL
                     KYNPDNQNALENAAFGYHIKNDNKKVQELAKKLTEIDKSSIIANQLMILSCDTLKEAE
                     DYFETIPLEIRHKDHVASAIAFKYYQDNDFENALKWYDIAIKERPQDRELIYLYAVAM
                     ASKINKKVHSNISLSEEDLNKGREIAEKIKKFWNEIPTKEEKINRIDWAVNGATLLAD
                     LGEFEESLTLLKEAENLIPNDFYIIRNLAVIYHKTGQTYKALEYFEKLLQKEKTPENI
                     ILYSNLIYNERIVEKFPTTIELLSELIEHIESDKQSKEKSISFKTIILCDLNKFNEAF
                     EFIDKYLKEDPSNPYHLLTMGIYHLKNKNYEKAAEFFEEAKSNISSNNDYFLRKMVAE
                     RLFNLNHFKSASEVYESLIKKPYVYDELTFRYITSLYYSDKKKELLEICKELRDQHEI
                     DEKITPFEINILEIKGDIKEAYNISELYLKKFDNIHERIRNAMILWRFNEDATNDKLD
                     GFLLDFDLVKIKDQPIELWKKLSFLFDKRSLFNSDIRRKYLDFTYEMRRNFYNDIETH
                     KLYYFNFITKFQDLIKKVETIENNCHVQIEIEGSGKFDFIIDDRPDVSVDRKEKMPDD
                     PIVQKVLGQKNGFEYTYRESPFGNNVAKIVGFESKYVFALRDALKIIEHAPDARGFYK
                     IKIPENEKGELDVPAFFKMLENGNKGWKKISEETKDFYTSRRIPLSFYAKAEQKNIIE
                     AWLKVTKDKALGVFSTTGIAKNVYPVLKKLLFRAEDQPIVIDLISIMTLIAIDDENII
                     SDSYGPFLIPQSSLDVINMISTQIPNTQKTLDLANKIKEWIKNNCRIENPSTAIKELE
                     EPMGKEFSDPPSLAKEKAAFYYSDDTIQRSFSETNGVWTELMLMDLLYKGKIDEKKHK
                     DMMVKLAEMYYYRTVVNAEILIYAVQKADGNINSTIEKLFHYFSANIYDEDFSILTGT
                     DFLIELLQQNFLSDFKKQTIINVFFSAFFENRMGRKMAVSKFEATIKNKFQYDPLKRD
                     YLIGMLFDWERIHLAV"
     gene            complement(19613..>19863)
                     /locus_tag="PL056_01815"
     CDS             complement(19613..>19863)
                     /locus_tag="PL056_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HPM45782.1"
                     /translation="VSSPKLGSINHTMMTLEMLKSRGIPVAALVYNMYPAEKIEIAND
                     SINVIKQFSKKLGLQFPVFITPFIDEKSNITVDFEGLV"
BASE COUNT         5571 a         3996 c         3672 g         6624 t
ORIGIN      
        1 cagcaataat tcgtgaaatt gacggagcag gaatggatga taatccaata aatctgattt
       61 cttacataaa taaattgaaa aaggagcgga ccaatgacta agctgagtaa aagttttgtt
      121 ttctacatga aaaattttga aacaatgcag gctttgaatg aacttgatga agaaaacgaa
      181 gatgaaatta attcactttt tgaaggatca ggatttaaaa atgcaagcga tatggttgat
      241 tcagtcactg aggtggcaaa tcaaactgct gcaagtattt tattaaagat tgggatggag
      301 gtagatgaaa aatatagttt taaaatgata agtcgtttca acaaaattaa agaaaattgg
      361 tttgtggaaa actggatgta caaaaagaga cctgaaacaa aaaatggata tgtgttaagc
      421 attcagattt gttcaaaagg agacacaaca gaaggaatag gttttttaag ttgttctatt
      481 tatgatagca gaaaaaaaga cactgcgctt ttagaaaaga tttttagtga aaacattaag
      541 gaagaatatg atgatatttt tgggaaaaac gaatggctta tacttgaaac agttatttta
      601 gaagaagata gcgaagagaa aatcattgaa gatttcatgg taaaactttc agaaattttg
      661 acacaggata atcttacaaa actttttgcg aaaccatgaa attttcataa aaattattac
      721 attgagagca agaaaccgtc aattgacaaa agctattaag ctcatataat aataaaggtt
      781 tttttaggag gagaagatgc cggagttaag cagattctat ggtattgtta ttgcaatgtt
      841 ttatgatgag cataacccac cacattttca tgctgtttat ggaaaacata aagttgccgt
      901 cagacttgat aattttgcag ttatttctgg aaaatttccg ccgagagcat tgggactggt
      961 aattgaatgg gcaatgctgc ataaagaaga acttgagaaa gcatgggaaa aactcatagc
     1021 acaggaacct ctttttaaaa tcaacccttt aaagtgaggc agattatgca cttcgatata
     1081 aaaaaagcag tttatctagg tggttacaag ataaagctgt ttttcagaga taagaaaatc
     1141 ggtattgtcg attttgaaaa ctaccctcca agaaaagggg tttttgagaa gtttaaagag
     1201 atcgaattct ttaagcagtt ttatattgac ccggatttca atgttcttgc gtggccggga
     1261 ggaatagaca tcgctccaga aacaatttac gaaatagcca caaacacaaa acaatcctca
     1321 taaacaccaa catataagaa agaattaaca gttaaattca gcaattattt caataaatca
     1381 tcccaactac ccggaatttc cggatagttg agatttgata tttaaaagga ttatcagttc
     1441 ttcttgagcc gtttctcgat ttctttgtag agtgatttga tgccgctttc gattgtgccg
     1501 taagcttctt ccatggtctt tttgatttct cttacgacat cgttatttat tgctgctctt
     1561 gctgctgcaa gccgtgcaat gggcactctg tatggtgaac agcttacata gttgagtccg
     1621 catctgtgga agaattctat tgatgcggga tctccgccgt gttctccgca gactccgagt
     1681 ttgatgttgg gtctggtttt ctttcctctt ttgcatgcaa tttcaacaag ctggccgaca
     1741 ccttcctgat ctattgactg gaaaggatcg atcgtgagga tcttcttttc aatatattct
     1801 ttcaggaatt tgcctgaatc gtcacggctg tagccgaaag tcatctgggt aagatcattt
     1861 gttccgaatg agaagaactc agcttcttca gcaatttcat cagcagtgag ggcgcctctt
     1921 ggaacctcta tcattgttcc gaccatatat tcaacagtga tctttttctc tttgaaaacc
     1981 tgctcaacag tactgatgat gatctctttc tgcaatctgt actcttcaac tttacctaca
     2041 agcggaacca tgatctcagg gaacaccttg acaccttctt ttgtaaggtc gcaagccgct
     2101 tccatgatcg ctcttgcctg catctcactt atctcaggat atgtgttgcc aagacggcat
     2161 ccacggtgac caagcatcgg gttgaattca tgcaattctt cgactttgct aattattttg
     2221 gcttcggtaa ctttcatttc ctttgccatt tcctttatct gtttcggctc gtggggaagg
     2281 aactcgtgaa ggggcggatc aagaagacgg atagttacag gaagtccttc catctctttg
     2341 aaaattccgt agaaatcgtc tctctggtgc ttgatgagtt tcttaagtgc ctttcttctt
     2401 ccttcttcat catcggcaag gatcatctct cttaccgctt tgatacggtc gccttcaaag
     2461 aacatgtgct cagttctgca aaggccgata ccttcagcgc cgaattctct tgcaacttta
     2521 gaatctttgg gtgtgtcggc gtttgttctt attttgagaa ctcttctttc gtcagcccat
     2581 ttcatcaatg tttcgaactc accttcaaat ttcggctctt ctgttgcgat cttgccgaga
     2641 aatacttcac cactcgttcc atcaatagat atccagtcac cctgttttac aacgatgtcc
     2701 ttaactgtga actgctgtgc tttgtaatca attacgatcg ctcctgcacc cgatacacag
     2761 cattttccca ttcctcttgc aacaacagcg gcgtgtgaag tcattccgcc tctagccgta
     2821 agaataccgt ttgcggcatc cattccgccg atatcttcag gacttgtttc tattcttacg
     2881 agaattgtcc cttttttctg ttttttccat tcaacggcgt cttcagcatt gaaaacaacc
     2941 attcctgttg cggcacccgg gcttgccgga agaccttttg cgattgcttt tgcttttgcc
     3001 ttttccgctt taacgaactg aggatgaagg agctggtcga cctgtgacgg ttcaaccctt
     3061 aaaagagctt cgtcttttgt gatcatccct tcatttacca tatcaacagc gatctttact
     3121 gccgcagcgg ctgttctttt gccgtttctt gtctgaagca tccagagctt tttgttttca
     3181 attgtaaact ctatgtcctg catgttttta tagtgcgctt caagccggtg ataaatgtca
     3241 cagagctctt cataaacttc aggcatatat tcttcaagtg acgggtattt cgattttctt
     3301 tccttttctt ttacactgtt gctttcagcc cagttgcggc ttcctgaaag agtgacctgc
     3361 tgaggagttc ttattccggc aacaacatct tcgccctgag cgttgataag atattcaccg
     3421 aagaacactt tttcacctgt tgcagggttt cttgtaaatg caacacccgt tgcgcaatca
     3481 gcgcccatat ttccaaaaac cattgcctga atatttaccg ctgtacccca tttgtctgaa
     3541 tatccttcca ttttgcggta gtaacaggct ctcggattca tccagcttcc aaaaactgct
     3601 tcaactgctc cccagagctg ttccatcgga tcggtcggga aatcttttcc ggatatcttt
     3661 ttaactatct ttttatattc agcaacaagt tccttcaagt gatctgcagt catatcaagg
     3721 tcgtttacaa gtttatattt tttcttcaat ccttcaattg catgttcaaa attttcaccc
     3781 ttaacttcca tgacgacatt gccgtacatc tgaatgaaac ggcggtagct gtcccatgca
     3841 aatctttcgt ccgctttagt tttgataaga cctttcagag tcgtttcatt aagtcccaga
     3901 ttaagaaccg tgtccatcat tccgggcatt gaaactctcg cgccgcttct gacagaaagg
     3961 agcagaggat ttttcttgtc accgaacttc ttgcccatga catcttcaac ttttttcagg
     4021 ttttccataa cctgttctct cagttcaggc ggatagttac ggttattttc gtaatatacg
     4081 gtgcacattt cggttgaaat tgtaaagccg gcgggaaccg gaatcttgag attgcacatt
     4141 tcggcaaggt ttgccccttt tccgccgaga atgtttttca tagttgcatt tccttctgaa
     4201 ccctcaggtc cgaaaaaata aacgaatttt ttaaccattt ttttctcctc taaatgttaa
     4261 gataactgat atctccaatg tttttaatta ctaatgtagt tactttgttt ataagtctca
     4321 ttctgttcat ccttatattt tcgtcttccg ccattacaaa aacagcgtca aagaaggaat
     4381 ccaacaccgg ctttattgta attattttca aagctatttc aagattgtct gacatattaa
     4441 cgatttcctg acaaagtgtg tcagccgctt taaaaagatt cttctcttca gccagttcaa
     4501 aaagggattc gactggttct ccaaaaatat tttcctgttt tttcaggatg ttacagcccc
     4561 tcttataaag ctgtgcaaga cctgtgatct cattgtcttt taaaagccgg tttattgttt
     4621 cagcccgttt tttaacaaca gccggaatgt ctgcgcctgt tgcggtagat gcctggatga
     4681 cgggcgtttc aaagttaagg actgcgttgt atcttgccgt catgaactcc attatttcgt
     4741 tcacatcaag tttgagtttc ccgttgtgtt caaggatgag tctgcccgaa aattcaaaaa
     4801 gctctcttag atttatggat gccttttcat tttcaactgc aagggtcaga agcgaaatgg
     4861 catttctcct gattgcgaac ttgtcttttg agccggtcgg tttcatcccc gccatgaatc
     4921 cgccggtaat tgtgtcaact ttgtcggcaa tcgaaagaac cgtccctgtc agagtcgatg
     4981 ggagtagatc tccagcattt acaggtagat agtgttcata cactgctttt gatatctcag
     5041 gatcaaatcc ggcatgttcc gcatagtatc ttcccataat gccctgaagg tcgggaaatt
     5101 caaaaacaac tcccgtcaca agatcgttct tgatcatttc agatgcggtc tttatttttt
     5161 caatctgact cttatcggct ttgaagaact tctgcgcagc aaattcagcg attttcccga
     5221 ttcgcactat cttatcgtga tatgttccaa gatctttctg gaaaaggact tcgcgaagct
     5281 tttcctttaa tccgtgaaaa tctttttcaa gatcatcggt aaaatagaaa gcagcatctg
     5341 caagacgggc tgaaaccacc ttttcatttc ctttcacaac aaccgaatca tcggtcggta
     5401 tgttatttaa aacactggca aattttttaa caacggtttt ttcaccctgt tcacacagaa
     5461 caaaatatct ctgatctttt ttaagaactt ttgtgataag ttctgccgga agatgactgt
     5521 atttttcagg gatcgtgccc acgataacat ggggatattc caccatatcc gtcacttcat
     5581 caagaagatt ttcctcaata tttgcaataa gttgattctt atctgcaatg gcgttgacag
     5641 attcattgat catctttttc ctttcatcac ggtctgcaat cacgaaatga cctttcagca
     5701 ctttctcata atcttgtgca gaaactgtca gcggtccgtt tgcaagaaac ctgtgcccga
     5761 aagaggtgag tgaaaaatcg acatcttcaa atttgtcagc gaccggtttg ccgtttattg
     5821 aaaggaatat ccatcgtatc ggtctagcga aagggaattt gtgctctccc caccgcatgc
     5881 tttttttgaa acttaaagct tttatcccgt ttttaacgat agggataata acttcttgaa
     5941 tttccatacc tttttcttct ttccagccgg ctatcacttc gcctttatcg ctgttttcaa
     6001 agaaatattc agttattccg ttctttgcaa ggaacgattc tcccgctttt gaaagagttc
     6061 cttcaggagt taaggatact tttttcggag cgcctgtaac gagttctttc cggtccggtt
     6121 gtttttcagg aagttcagta attactgccg caatccttga acatgttgaa tacatttgac
     6181 attttgcatt cgcaagccgt gctttttcaa gctctgaaat aagaaaagcc cgcatctgtt
     6241 cagctgcagg aacaataaaa cctgccggaa attcctcaaa gccaatttca aaaatgaagt
     6301 gtgccatcat tttcctccga cattagtgaa actttcggca tattttccgg ctattgcaca
     6361 ggcgagatcc cttattcttg tgatataccg ttgtctttcc gttactgaaa ttgcgcttct
     6421 tgcatcaaga atattgaagg catgacttgc tttgatgcag aattcatatg cgggacgggg
     6481 gagtgatttt tcaagaagtt ccctgctgat cttttcatat ttatcaaaag aatggaaaaa
     6541 atcttcaacc ggtgcttcct tgaaattaaa atgtgagaac tcaacctcat ccattttatg
     6601 aatgtcgccg taagttatcc ggtcattcca tttgagatca taaacattgt caacatcctg
     6661 cagatacatg cagatccgtt caagtccgta tgtatattca acaggaacgg ggtgaagatc
     6721 gatgccgccc acctgctgaa aatatgtgaa ctggcttatc tccattccgt caagccatac
     6781 ttcccatcca agaccccacg caccgagagt aggactttcc cagtcatcct cgacaaagcg
     6841 gatgtcgtga tctttcggat cgatccctat cactttcaag ctgtcaagat ataactgctg
     6901 agcattttca ggacttggtt tgacgagcac ctgaagctga tagaacctct gcattctgtt
     6961 cgggctttcg ccgtatctgc cgtctgtggg tcttcttgac ggctgaacat agacagagct
     7021 gatgtttgca gggttaagtg catagaaaaa tgttgcagga tgaaaagttc ccgcacccat
     7081 ttcgatgtcc cacggctgaa ccagaataca gcccttttcc gtccagaatc tctgaagccg
     7141 gaaaatcatt tcctgaaagg tgattccctt catttttccc cccataaaaa agtcgcggag
     7201 gactatatca catttaatgc tcaagtcaaa aatgtcaggt gataaaaatc gccagttgat
     7261 taaaaattga ttcacataaa aaaaagcata atctttcttt tgttatttga ttcatttttg
     7321 gagcgatcta tgaaaaaagg ggttgttttt caggaaagtc agcggtttac gcagtggtgg
     7381 ctttggctcg ttgtgatagg gatggactta atgtatttat ttgctgtggg gaaacatctg
     7441 ttttcccaga tccagtcagg aaaagagccg cagataaatt cccagataat aatcgctgtc
     7501 acaattgtct ttttaataac agtccttttt cttcttttac ggctggatac aattattgat
     7561 gatgccgggg ttcatgtgaa atttttccct tttcaccttt cgtttcgaca ttacggctgg
     7621 gaaaccattg cgaactgttt tgtcagaaaa tacaagccgt tgctcgaata cggcggatgg
     7681 gggataagag gcacgggcaa aaataaagca ctcaatgtca agggaaatat gggaatacag
     7741 atagaattcc atgacggcaa gcgactgttg atcggaacat caaaattcga agaagtttca
     7801 aaatttctga caggaagata acatatcaga tcagatccac tttttttcag cttgagcgag
     7861 cagattggat atctgtagat gaccttttgc ttttgctata tctgaagcgg ttttcccttc
     7921 aacctgcctg atcgtggggt cagccccttt ttcaagaagt tttgaaacga ccgagaacag
     7981 gttggcttca gcggcgaaca tcaacgccgt gttgccgaaa gagtcaattg tgtcaatgtt
     8041 ctgctttttg tcgacgagca gatttatcag ggaaggattg ttatttgtga ttgcaagcat
     8101 caaagcggtt tttccatctt tgtaccggtg gttgaaatta gccgttgcac ctgcccttaa
     8161 aagatgtacc gcttcattaa atctgccgtt atgcattgca aatacaaaaa gagttgcatt
     8221 tttgttctta aaataacttc cgttcgggtc agcccctttt tcaagaagaa atttcaaaat
     8281 gttctcatga ccgttttcga ttgcaacaag tattgccgcc ttattgttcc tgtcttttgt
     8341 gttgatgtct gccccgtcac caacaagttt ttttgcaaga tttacatcac ctttttcaac
     8401 tgcttcaatg aaatccatcg gaagtgtgtc aagtttgctt aagatccttg aacactccgc
     8461 cgcccttgca tcaactgcat agtcaagtgc tgttttatcg gcttttgatc tgacttttct
     8521 gtcaaaaccc atgtcaagaa gaagctttaa taattccgga ctgttacctt ctgcggcata
     8581 catcattata ttcttgttgg atggatcttt tgctttaagg tctgagcccg attttacaag
     8641 cagggcggta atttcagcat tctttagcga cagcgcatgc atcagagccg tctttccgtc
     8701 agggcctttg agttcagtgt tcacgctgtt attaagaaag aaacggacca tctgaatatc
     8761 gtttgaagtt acggcggtaa tgaataacgg ccactcttca tcaacatagt ttatatcagc
     8821 cccttttttc agtgcttctg atgccgcaac acaatttcct gcctggatag cggcagagaa
     8881 ttcaatgtca atttcaccag cagatattac agaagttaat aagaatatta ggaaaaacaa
     8941 tctcttcact cgtcacccgt cactgttcac taatcactta ctcgcactgt gtttcatccc
     9001 agccggtaca gtctgaatta cagtaggccc atccgacagt ataagatcta tctgttatat
     9061 ctttgcattc gatactgttt ccgtcacaga tctcttcatt gttgaccatg tagtcgccgc
     9121 aaaacggttc cggtctggtt cctcccgtca gagttctttt tcccatcggg tcatcaccat
     9181 taagttctat gtcaagtttg tcgtcagtgt gaagcgtgaa aacggcggtc atatttcctg
     9241 tcttttcaac ctgttcgacc cagatgctga attcgccggt gaaagtgtat cccacaaaac
     9301 tgttctcatc cccgtgggcc ataaaaaact cggtataatc attgatgctg atgtcaagca
     9361 tgtatccgct acctgcttct tcgtttttca taacggaaac atgggtagca ttcgggaaat
     9421 cccactcgcc cagaagatca tccggatttt tcggagtgac atcttcatcc ggtattatat
     9481 cgtctatagt ttcatcgtca gtgtcttcaa tatcttgaac ttcagtatca cctgtctcat
     9541 cgtccggtct aactgcttca tcatttgtct ggtcttcatc ggtaaggtct ttttcatcaa
     9601 agatcattac tgagaattct gagaagtggc ctacttcaga catgatggga tctccactca
     9661 ttatcggatc tcccgacata atggggtcgc ctgacataat gggatcccct gattgttcaa
     9721 aaccgcttcc tgcataaacc cacttgtctt ccttatacac tgcaacagtg atgatctgtc
     9781 ctgcaacttt gtttttagtc gttttcaaag tgagtgtggc atttttctta aactgaagac
     9841 cgtcaggtcc gaaatcgtag atattgcttc cgagatattc cttgtcggga tacttagaag
     9901 ggtctctttt ctggatggtt atgtttgtat ctgattcaag agcatcggca ggaatatcaa
     9961 gtttcgcaag gccgtcagcc gattcgatcg ttccgccttc cactgctttt ataagtttag
    10021 ttgttatttt gtcatagtca tttgttccag tgcttgattc tccgcatgat aaaaatacaa
    10081 acatcaaaat tgaaatgatt gcaatcagtg attttttcat aacatcccct ccgaggaatt
    10141 aaagaaataa aacggcaaat tatatcagaa ccgctaaaat ttgcaagatg aagtatagta
    10201 ttgtatattt caccgtactt actgttcagg ggtgtaaaat gacagagatg tttgttttta
    10261 ttgagaaagg tgtttctgaa ctgggtgctc ttgcctgggg tccgccgatg ctcgtttttc
    10321 ttgtcggaac agggatctat ctcacaattc ttcttaaagg tcttcaattt tccagactgt
    10381 tttattcgct gaagctgata ttcgtcaaga ttggagatga ggagaaaggg catggcgata
    10441 tatccaattt ccaggcgttg atgacagcgc tttctgcaac ggtcggcaca ggaaacattg
    10501 caggcgtggc aacagcgata gccgcaggcg gtccgggtgc tcttttctgg atgtggatga
    10561 cagggctggt cggaatggcg acaaaatatg ctgaagcggt ccttgctgtc aaatacaggg
    10621 aaaaagataa gcacggagct atgtgcggcg gaccgatgta ctacatttca aaagggcttg
    10681 ggcaaaaatg gctgggaact ctttttgcag tttttgccgc tgttgcaact ttcggaatag
    10741 gcaacatggt tcagtccaat tcagttgccg ccgcgatgga aagtactttc ggagtatcaa
    10801 gctggataac gggagccgtt ctgatggttt tttctgcttt tgtgcttctt gggggcataa
    10861 aaagcattgg aaaagttaca gcggtacttg ttcctgtgat gatcgttttc tataccggat
    10921 atgccatgtt tataattttc agcaatatgt cgcagatccc ttccgccttt gccataattt
    10981 tcgagtatgc tttcactccg gtagcggcta caggcggatt tctcggttca acagtgatgc
    11041 ttacgataag aatgggtgtt gcaagagggg tgttctcaaa tgaatcgggt cttggaagtg
    11101 cacctattgc agcagcagcg gccaaaacaa atcattctgt acatcaggcg cttatttcca
    11161 tgacacagac ttttatagac acacttgtcg tctgcacaat gacaggactg gttataatca
    11221 tgtcaggtct gtggaaagac gggaagacgg ggtccgatct gacagcgtct gctttttcat
    11281 ggggaattgc aggagggaac attgtggtca ctgtcgggct gatactgttt gcttattcca
    11341 cgcttctggg ctggtgctat tacggagaaa aatcgattga atatcttttc ggtgaaagat
    11401 cggttaaacc ctacagaacg gtttttgtaa tactgatact agtcggtgct atatcgaaac
    11461 ttgatttcgt gtggtcgctt tccgatactt tcaacggttt gatggcggtt cccaacctta
    11521 ttgcgttgct gatactttct ccggtggtag tttctgaaac gaaagattac tttgcaaaaa
    11581 taagaaagcc ataaggagag agcatgaaaa atgtcagaac tctgatactc ggagcggggc
    11641 ttgccgggct ttcagcggcg tatcatctga aagggaagga ttttcttgtg attgaaaaaa
    11701 gtgatcatcc cggcggtctt tgcacaactg aacataaaga tgggttttcg tttgatcaga
    11761 ccgggcactg gcttcacatg aaagatgaaa ggacaaaagc actttttaac acgcttttta
    11821 atgatgaatt tgttgaaata gtccgcaaaa ctttcgtttt ttcacacaat gttttcacac
    11881 agtatccgtt tcagtcaaac acttacggac ttccttcaga tgtcattaaa gagtgtgttc
    11941 tgggttttat taaagccaag tatgaaaatg acaagtcaaa agcgggcgac aatttctatg
    12001 aatggtgcat ggcatatctg ggtgaaggca tttcaaagca tttcatgatc ccttacaata
    12061 cgaaaattta caccgttcat ccgaaagatt atgcgagtca ctggtgcgat tactatgtgc
    12121 cgaaacctac tctggatcaa gtaattgaag gtgctgtgac ctctcctgag cagaaaaatg
    12181 tgggttacaa cgcgacattc aaatatccgg aaaatggagg aataggggag ttgccgaaac
    12241 ggctttttga taaatgcgaa aaagagaagt ttctgttcaa tgttcatccg gttgaaataa
    12301 atagtgatga aaagacaact ctgctttcaa acggagaaat cataaagtac gaaaatctaa
    12361 taaattcaat acctttaaaa gattttctca aacttcagac tggatcggca gggtcgtgga
    12421 gcaatgaaat atcagacaga atgaagatcg catcggtaag ttatctcaat gttgcattca
    12481 atagattgcc tggacacaca ggtcactggt tctacattcc tgaagaaaag tttatgcctt
    12541 accgaatagg ttctttcagc aatatctatt caaaactcgc tcccgacgga accggttcag
    12601 catatatcga atacactcat caaggcgatt tttcagaaat cgaactgctt aaacagaaaa
    12661 gtgtagaact tatgctcgaa atgaaaatga taaaaagcat taacgatatc aaatttatgg
    12721 attaccgcaa aatcgagaac gggtatgtca tataccacaa agaatatttc gaagatctca
    12781 aaagaattga aaaatggtgt gctgaaaatt caataaaact tgccggcaga tacggcaaat
    12841 ggacttatgc cgcaatggaa gatgcaattc ttgacggaat gtccgctgtc agcggtcttt
    12901 acaacatctg aatccggcaa aaagctctga ccatgttgcg ttgtatccgc attcaagacg
    12961 gctcgaaggg ctcccgcagt tgaaagcgcc gcctcttatt ttgtaagcag agccggtatc
    13021 ggttatttcc cacacatttc cgtttatgtc aaatgctccg gaatgattta tgcagttaag
    13081 aaaatcgcca gtttttacaa cattgaagca gtaataactg tatccgcagt tttcagacat
    13141 aagacagctt tcaggtgcaa ttgaatttgt ttcgcagtgt tcatcacaga aagcgtctac
    13201 cgtgttacat atttctctgt tgtatgtgcc gccccacgaa tattttcttt tttcagtccc
    13261 ttcgcatgaa aatatccact catcatctct gcaaagacgc tttcctgcag cggtacaggc
    13321 agttttaaat ttctgataat cagccgcagt catcgggttc accatccatg gaagaacact
    13381 tttttttgaa acggcaacag attcgtcaga cccgtgagag atatctgtag catctttttt
    13441 tgatgcctca tatttgtcaa tgcagaaagt tccgttctca accatgtcag aagggcaacc
    13501 gtcagcaatt gcatcagaat catcagcaat atcagaatct tgaatatcat ctccggtctc
    13561 aatatctgtt tctgctacag ttgcttcatc actgtcaggc agaaattcct catcatatgt
    13621 aacattttct tcttgaattt cgtcggggtc actttgcgtt ttgtcaggtt tctgaatgtt
    13681 ttcatcagga attacgccgc aaccaccctc atcgtttttt tctgtttctg actcgtcagg
    13741 atagttgtcg taaatggcag gatccttttt ttcagcacaa ccattgataa caacaaaagt
    13801 aaaaacgaca gtgatcatga cttgaaaaat aattctcata actgactccg tgataatcta
    13861 agcaccatct gcatagattt tatcaacacc tgtgcagttg ctaaatcaac cggaagggag
    13921 ttgtcttcct gcagatctat attcctcatc aatttccatt agtcctttaa cgattatggg
    13981 attatcattt tcaatttgtc taaaagcgga agcaaaaacc atgttaaaat aaaaggagga
    14041 caaaccatct tgagaaatat cgttatcaac gatcagataa aaattcttag aacatgccgc
    14101 atattctgaa agctcttctg gagacagtga caactctttg tggtttatga tatagttcat
    14161 aatcaggttt gaaccagcaa tcttcttagg aattgcgact ctaatttttt cgagagattc
    14221 ttcaatcggt tttggaggag ttctgtgggc attttcaata atttttacaa cacttttcaa
    14281 ttcaagaaaa tcaagtctat tttgttcaca tataaatttt aaggcatcgc aggatttagg
    14341 gcggtctcct atgtcatggt ctctacacac tcttattact gtatcatcaa aatcaatgac
    14401 atcagttttt gtgttttgaa caggtgtgga atttaaaagt ccaatctttt tatattgaaa
    14461 gtatcctttg ttttttccaa actcctcaat tttagagagc atatttcttc ccccacacta
    14521 tatcatttaa gggttttgac agttgaagaa atattttatt tatgtcttca gaaacattct
    14581 caatttccgc tgttccatta tcatttttat gagaaagata aaagtcggat ttaatatccg
    14641 cttcctttga aaacactgaa aacgcctgaa ccatgtaagg actatgtgtc gttacaagaa
    14701 ctatcacgcc ttctttaacc agttcagtga ttatttttgc atattctatc tgccattttg
    14761 gatgaagatg aacttccggc tcgtcaatta tcagaacgca tcttttttca agaacgcctg
    14821 cttttttcag tagcgatata attccgaaag atttaattcc agttgccgtg ttaagtgaat
    14881 cgaaagaatg tttttctcct gatatttctt ttgaaaagat aaaatcatct ttttcggtgt
    14941 cgtaatccca atctccttga attgtggatt caattttttt caaaaaaata tctgtgtcat
    15001 catcaaaata ccccatgcta ccggttagct tttcaaaaag atcggtaata tgaacaggaa
    15061 gagatttgtt gtagtttcca aaaagacctt ccatttgtaa aacaaaggga gtttcaatta
    15121 aagtcgcagt tggaacaagt aggttgtcag aatattcaac ttctatattt ccatttgata
    15181 gttgtatgtt taagagttca tttgatccct ctgaaaagga tatcttccca ttatctttag
    15241 aatgcatatt tcggatgttg taattgaact ccttttgcag gatttctttg taggttttac
    15301 tttgtatttt atttttgtct tcagtgtctt ttatccctaa aactgttctc aaatcctcaa
    15361 gtttaggaag aagaatattc agcaaaggtt tatttattga agatgtttta agaagagaga
    15421 ttcttttctg aataatatct tcgattttaa acacattttc agcaacaaca gcacttcgca
    15481 gctcttttat aaaagaccct ggattcagaa tgtcaaacag aattctgttc ccttctttat
    15541 ccagattttt tcttatttct atatatattt cctgaacaat atctttaata tttctttcta
    15601 attcgtccgt gaaaacaatc cctgaactat tagatgcttt tattatcgaa aaaagtgttc
    15661 ttcccgcaaa actttttccg gtgtcattgt ctccggctat cactgtcaat ccatcaagat
    15721 ttatatcagc ttgattgatt ataccgatat ttctaagact cagtttcatg ttgtcctcct
    15781 aaactgcaag atgaatcctt tcccagtcaa aaagcattcc tattaaataa tctcttttta
    15841 aaggatcata ctggaattta ttcttaatgg tcgcttcaaa tttgcttacc gccatctttc
    15901 ttcccattct attttcaaaa aatgcgctaa agaacacatt aatgatagtt tgctttttga
    15961 aatctgacaa gaaattttgt tgaagcagtt caataagaaa atctgttcct gtaagtattg
    16021 aaaaatcctc atcataaatg ttcgcagaaa aataatgaaa caatttttca attgttgagt
    16081 ttatattccc atctgctttt tgaacagcat aaatcaagat ctcagcattg actacagtcc
    16141 tgtaataata catttctgca agcttaacca tcatgtcttt atgtttcttt tcatctattt
    16201 tacctttata gagaagatcc atcagcatca gctctgtcca gacaccgttc gtctctgaaa
    16261 aacttctctg aatggtatcg tctgaataat aaaaagcagc tttttcttta gccaaactgg
    16321 gggggtcaga aaactctttt cccattggct cttccaactc tttgatggca gttgatggat
    16381 tttcaatcct gcagttattt ttaatccact cttttatttt atttgctaaa tctaatgtct
    16441 tttgagtgtt aggaatttga gttgaaatca tatttatcac atcaagagaa gattgcggaa
    16501 taagaaatgg accgtaacta tcagaaatta tattttcatc atcaattgca ataagtgtca
    16561 ttattgaaat aagatctata acaattggtt gatcttcagc tctaaaaagc agttttttca
    16621 acacaggata aacatttttt gcaattccag ttgtggaaaa aactccgagt gctttatctt
    16681 ttgtaacttt taaccaggct tcaataatgt ttttttgttc agctttggca taaaatgaaa
    16741 gcggaatcct tcttgaagta taaaaatcct ttgtttcttc tgatattttc ttccaacctt
    16801 tatttccatt ttcaagcatc ttaaaaaaag ctggaacatc cagttctccc ttttcatttt
    16861 ccggaatctt tattttataa aaccctctgg catcaggagc atgttcaatg atttttaaag
    16921 catctctcaa agcaaaaaca tatttgcttt caaaaccgac aatttttgca acattgtttc
    16981 cgaagggaga ctctctgtat gtatattcaa atccattttt ctgacctaaa actttttgta
    17041 ctataggatc gtcaggcatc ttctcttttc tatcaactga aacatcaggt ctgtcatcaa
    17101 ttatgaaatc gaactttcca cttccttcaa tttctatctg aacatgacaa ttattttcta
    17161 ttgtttcaac cttcttaatc aaatcctgaa attttgtgat aaagttaaaa tagtaaagtt
    17221 tatgtgtttc aatatcgtta tagaaatttc ttctcatttc gtaagtgaaa tcaagatatt
    17281 tacggcgaat atcactgtta aacagacttc ttttatcaaa tagaaaactc aactttttcc
    17341 agagttcaat cggttgatct tttattttga ccagatcaaa atctaaaaga aatccgtcaa
    17401 gtttatcatt cgtggcatct tcattaaaac gccacaatat catagcattc ctgattcttt
    17461 catggatgtt atcaaatttt ttcagataaa gttctgaaat attgtatgct tccttgatat
    17521 ctccctttat ttcaagaata ttaatttcaa aaggagtaat tttttcatca atttcatgct
    17581 ggtcacgaag ttccttacaa atctcaagaa gctctttttt tttatcagaa taatataaag
    17641 aagttatata cctaaatgtc aattcatcat aaacataagg tttctttatt aaactctcat
    17701 acacttcgga agccgattta aaatgattca ggttgaacaa cctttccgca accatttttc
    17761 tgaggaaata atcgttgttc gaggaaatat ttgatttagc ttcctcaaaa aattccgctg
    17821 ctttttcata gttcttgttt tttaaatgat atattcccat tgttagaaga tggtatggat
    17881 tggacgggtc ttctttaaga tacttatcaa tgaattcaaa agcttcattg aatttattca
    17941 gatcgcagag gataattgtt ttaaaagata tggacttttc tttagattgt ttatcagatt
    18001 ctatatgttc aatgagttca cttaataatt caatagttgt cgggaacttt tcgacaatcc
    18061 tttcattata aataagattc gagtaaagga ttatattttc cggcgttttt tctttttgca
    18121 gcagtttttc gaaatattca agggctttat aagtttgtcc cgttttgtga taaataacgg
    18181 ctaaatttct aataatataa aaatcgtttg gtattaaatt ttcggcttct ttaagaagag
    18241 taagagattc ttcaaattct cctaaatctg caagcagtgt tgctccattg accgcccaat
    18301 caatgcggtt aatcttctcc tctttagttg ggatttcatt ccagaatttt tttatttttt
    18361 cggcgatttc tctaccttta tttaaatctt cttccgataa agaaatgttc gaatgaactt
    18421 ttttgttgat cttacttgcc atggcaaccg cataaagata aataagttct ctatcctgtg
    18481 gtctttcttt tattgcaata tcataccatt ttaaagcatt ttcaaaatca ttgtcctgat
    18541 aatatttaaa cgctattgcg cttgcgacat gatctttgtg gcgtatttct aaaggtattg
    18601 tttcaaaata atcttcagct tcttttaatg tatcacatga aagaatcata agctggttag
    18661 caataatact tgatttgtca atctcagtaa gttttttagc aagttcctga acctttttgt
    18721 tgtcattttt tatatggtat ccgaatgctg cattttccaa agcattctga ttgtcaggat
    18781 tatatttcaa tgcttcaatt aaatagtcag cagctttttc ctgattccct acaccaatat
    18841 atgcaacccc taaattatta aaatatttcc acttcttttc attgctatct aagtgtgttt
    18901 tattaaattc ctcaagccag attctcgctt tatcgtattc cttctgatca agtaattctt
    18961 ttgatttttt taaaaattct ccagaaatat caatattgtt tttaacttgt aataaatcca
    19021 tcacgctgtc aagtttcttg cttatcagat cttgactatc ctgaagcttt acctctgatg
    19081 gtttcattgc ttctttaatt gtttcatcaa atattttgga aaatctctct atttcaattt
    19141 taagatcatc tacatttcta tatccgtcac ttcttatttt cataaagcgg tcgtcaccga
    19201 tatttagata agtgtttatt ctatcctcaa attcatcgta gttgcttgag aatttcatcc
    19261 tgaatgcttc ccatatctct ttttttgcaa aaaagtccgc aaaattcttg tttttgcatt
    19321 ccacactata tttgaccaga gtatgagcat atattgattc aaattcattt tcgggtctaa
    19381 tggaaacacc acatttttta agcaaatgta caatcttttc attccttgaa acagattcca
    19441 tggttttttc aaaagcgaac tctgctattt tacttaaaac agaaaaaccg atcttcgtta
    19501 aaaccgatga agtgaccata aacttccctc cttaaataac taatcgaaat gatagttatt
    19561 tttgataaat ggtcaaagaa acagaaaaat aatactttat tttatattaa tctcagacaa
    19621 gtccttcaaa atcaactgtg atattggatt tttcatcaat gaaaggggtg ataaacacag
    19681 ggaactgaag tccgagtttt tttgagaatt gcttgataac attgattgaa tcattagcaa
    19741 tttcaatttt ttccgctgga tacatgttat aaacaagtgc ggcaacaggg atacctctgc
    19801 tttttaacat ttcgagtgtc atcattgtgt ggtttatgct tccgagcttt ggtgaagaaa
    19861 cca
//
LOCUS       DAOOYB010000016        19610 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_2658_length_19610_cov_10.776477, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000016 DAOOYB010000000
VERSION     DAOOYB010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 19610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19610
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2658_length_19610_cov_10.776477"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="PL056_01820"
     CDS             <1..638
                     /locus_tag="PL056_01820"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HPM45783.1"
                     /translation="EIDQAQRLYQISRFNTAALPFYAIIDPSTDTVLAVHPDMTNDEN
                     KFAEFLKNGIASFNPAENVVEEKKENTGTDFTFKTIDGAKSVSLSSFSGKWVLLNFWA
                     SWCAPCREELEHVFPEVLKSYPDVLFMTVAFDGEQSAHAAKSFIDGIRIPGIVNLLGP
                     EDPTSAGLSEEYKFEGSLPSTYLISPEGRIVWMKSDVVDGKTLRKELEKTR"
     gene            complement(668..2347)
                     /locus_tag="PL056_01825"
     CDS             complement(668..2347)
                     /locus_tag="PL056_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015905170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HPM45784.1"
                     /translation="MFIPATVEELEKLGWDECDIILVTGDAYIDSPYSGIAIIGHILI
                     AKGYRVGIISQPDMKSGEDIMRLGEPLLFWGLTSGCVDSMVSNYTALKKRRSDDDLTP
                     GGVNDRRPDRALIVYTGLIRRFFKNTVPIVLGGIEASLRRIAHYDYWDDAVRRPLLFD
                     SKADILVYGMGEKAILELADSIKNEKDYDSIKGLCVISKEPVEGFVQLPPFEKVVENK
                     KTFSDMFMTAYRNNDPLTASGMCQKVGERWLIHNPPQPLPTSIELDSIYELDYEYDAH
                     PKCRERGEIRALETIKFSVTTHRGCYGECSFCAIAMHQGRTVISRSEDSIEREIKRFL
                     TKKKFTGIIYDLGGPTANMYGFECEKKMKEGPCRKKRCLHPEICQNMKIDHSRQIRLL
                     KRISNIPGVKKVFIASGVRHDLVLADKKHGTAYLKELVEKHISGQMKIAPEHIENTVL
                     SLMGKPSQKHLRRFRELFNELNRVADKKQFLTYYFIAAHPGCTNKDMESLKDFSRREL
                     KMKPEQIQIFTPTPSTISTLIYHTETDPYTGHNVFVEKDINRKKIQKKIMS"
     gene            2439..3335
                     /locus_tag="PL056_01830"
     CDS             2439..3335
                     /locus_tag="PL056_01830"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPM45785.1"
                     /translation="MDFLKLSNHQKGIVLLFINAFLWGIHGPAGRFLALRGVDMYFVA
                     GMRLLIGAAVLFLFLAARKGITFSIKGHVKDILIVSIVGLFLNTLFYHLALKWISGTL
                     VMVIENLSPVFVIFFIWAISKTKPNKNQILALILSFAGMIVIVAGKDKLSVNGIDSLP
                     GIIFGILAGITLAFYYFFSGELISKVKDDPNKMLSLLMIIFLIAAIPMLPLVISAQNL
                     PSKFDEWFWLIEMGIFQSGVAYITLNLAMRYVSASTASIFFLFTIFFTTMNELIFLDL
                     QLNPYLIIGSILIILAARKASV"
     gene            complement(3356..5755)
                     /locus_tag="PL056_01835"
     CDS             complement(3356..5755)
                     /locus_tag="PL056_01835"
                     /inference="COORDINATES: protein motif:HMM:NF012632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45786.1"
                     /translation="MKRNFLIVIVVTFFLMMSISCENAENSGKVGDDTDNSEYTDMTD
                     EKSDDADSYDVDNEDEDENENDEEIDDDNDNSLWLKIEAGYYHTCGIHSENKSLYCWG
                     ANSYGQLGDGTDGYECMNHKNEPGECSDKTVPIKVDNEEWLEIAAGAFHTCGIKKDKK
                     LYCWGSNRFGEIGDGKKTTSDENNDRNIPVKVNDDEWIAVTAGYYHTCAIKTDDTLYC
                     WGLNVFGQLGLGDEMTGDALIPAKLNDDKWISVTAGGLHTCGINKDNNLYCWGKNSFG
                     EVGDGTNENRFISTKIGNDKWKSVSAGHSHSCGINIDGRIYCWGDNILAKIGDGTETV
                     YGEELTILWNNNKNIPVKISNEFWASVNAGGSHTCGIHEDKSLYCWGGNNAGQAGDGK
                     NGYERYVPTKINDDKWNFITTGSEHTCCVKSDGNLYCWGLNSNGQIGDGENDFKKTVP
                     VKVDDDSWVQVNAGFSDNCGIRSDSKLYCWGFNSYGQIGDESSDYNKPFPVKVDDSSW
                     KVVENGYYHNCALKTDNKLYCWGYNGNGQIGDDTITKVSEYGTIEEQNNKTVPVKIGE
                     DLWSSVSCGVYHTCGIKTDKKLYCWGDNSYGSLGDGTEEGRTVPTNVDDSEWISVFAG
                     YYYSCGIRSDNTLYCWGENELIPTQIGKDKWIAITAGYFHSCGIKTDKKLYCWGENNF
                     GELGDGTEEYKSDPTKVGDDEWIKVSCGMNHTCGIKSDNKLYCWGDNQNGQLGNEENT
                     AKNVPTKVGDDDWIEITAGLLHNCGIKADKKLYCWGDNTYGSIGDGTSGTQSTPVLVE
                     K"
     gene            complement(5884..7086)
                     /locus_tag="PL056_01840"
     CDS             complement(5884..7086)
                     /locus_tag="PL056_01840"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HPM45787.1"
                     /translation="MRVFFAFIFILIFASGCKSEIINKNNTDSDSLLVVDIDIVDDNY
                     ELFNDDEDAKIDDIQDEKQDEYFDEQFEEDISESIDERDEENTDLDADILPESTGRTI
                     CTGQISCYNNTEEIPCPLSGENFSGQDAQYIEKCIPRSYAVSISEGDEIITDNNTGLM
                     WQRTIPETYAGCAFGDPVGSMCSWQEAINYCNDLDYGGYDDWRLPSRIELESITDYGK
                     SNPSIDTDLFIDTPVKWILTTTSFVMDSKRKWSVNQGVGTVGTRNQTDETYVRCVRGN
                     AIYLSNFVEFAVEYKIIVKDLKTNFEWAKEAVSGGTWETVLNYCDNLEYAGYSDWRIP
                     NVNELSTLFYDRAYQPASNFPGIYSEYYWTASTAVSANSSAWRINMFAGGVDLGMKSE
                     KHFAMCVR"
     gene            complement(7193..>7381)
                     /locus_tag="PL056_01845"
     CDS             complement(7193..>7381)
                     /locus_tag="PL056_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012926393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA-phosphatase"
                     /protein_id="HPM45788.1"
                     /translation="AAAGILRITAGEHYPTDVIAGAVLGSLTGLFIPLLHKIKNKNLS
                     VIPVVSSEYGGLQAVVNF"
     assembly_gap    7382..7481
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7482..7747)
                     /locus_tag="PL056_01850"
     CDS             complement(<7482..7747)
                     /locus_tag="PL056_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45789.1"
                     /translation="MQSNCSNKITVFIAVFLFTLTALSLEKNSDEFPFELTDAKEAFI
                     FSAGLGLMTSFMFTPSTDFEKSEILALDKYDLNAFDRSAVSMND"
     gene            complement(7751..8971)
                     /locus_tag="PL056_01855"
     CDS             complement(7751..8971)
                     /locus_tag="PL056_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007292558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM45790.1"
                     /translation="MFEREQYLKKIEPFMEKPIIKVVSGLRRCGKSTFLKMLINRIIR
                     NGAAEKNIVYISMELMEFEFITDYRVLYEYVKKKIQKNNKQKYLFIDEVQDIPGWEKA
                     VNSILAEGVFDIYLTGSNSRMFSSEFATLLTGRYIEIEMFPLSFKEFLQFRGMEDQSK
                     NEIEFETYLKYGGLPGIHNLKFDDVVVFQYINSIYNTILLKDIVARYDIRDISLLEKI
                     TAFVFDNCGSITTAKKIADYLKSQHINAGVGTVSNYINYLKDAYLAYKSGRYDLKGKK
                     HLELYEKYYMSDIGIRHSVLGYKKGDIAGLLENIVYLELLRRGYKVSTGKFDDMEIDF
                     VATRNGEKTYIQVTYLLASKETEEREFKPLEKINDNYPKMVLSMDKIWGDDRNGIVRK
                     NIIDFLLENLSPGA"
     gene            complement(9088..10515)
                     /locus_tag="PL056_01860"
     CDS             complement(9088..10515)
                     /locus_tag="PL056_01860"
                     /inference="COORDINATES: protein motif:HMM:NF013776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM45791.1"
                     /translation="MFFGRLSELKKLEKNFQSKKSELVVIYGRRRVGKSTLIKQFLKD
                     KPCHFIFEGIEGSSSAKQMEYFKDRMAEFTKDSLLEDMKFSGWEKVFKYFTEKMVSRK
                     NNKEKMVVFFDEIQWMASGRKQLISIIKYFWDNHWKDQNVMLILCGSIASFMVDKVIN
                     STALYGRVTEEMLIKGLNPDEIASFFKGKRDKYEILNYQLIFGGVPKYFEEIDISASF
                     YKNINRLCFSPNSLMLNEVEKIFYKQYSKAETYLDVVNLLKNGAFSFKEITEKIAKKS
                     GGSLKKVLLQLEKAELIESFVPFEQGWNTKFRKYRLADEFLRFYFKYMEPNMTAIQNQ
                     KNTTKLFEKISADSLDKWFGFAFERFCLKHSWYLAEIMGFADETLYASPYFGKNDSKF
                     QIDLLYKRADKVITVCEIKHYTGKITTKIIPEVEKKCALLKIPNGYTLEKALISLYGP
                     DEALKDSEYFNHFVTLEDIFRQKRS"
     gene            10655..>11772
                     /locus_tag="PL056_01865"
     CDS             10655..>11772
                     /locus_tag="PL056_01865"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPM45792.1"
                     /translation="MSFSGIRNIFRALSYKNYKIYFFGQITSQVGLWMQQVALGWLVY
                     RLTNSAFLLGIVGFSAQIPSFILSPIAGIAADRYDRYKILIITQSLFMVQALVLAALV
                     LLDIAGVTTIIILSLIQGIIMAFDAPARHAFVVQIVDRKDDIGNAIALNSTTFHASRL
                     VGPPLAGVIIAVANEGICFLINGLSYIMVIAALFMIKVKPLEHTGTSKSAINDIRDGF
                     IYAFRSVPIKAILLMVAFVSIFGMPYTVLLPVMARDVLHGTSQTFGILMGGAGVGALT
                     GALYLASKKNAHGLFKNLAIAPLIFGLGMIGFSMSRSVELSLILLVTGGFGMMTTMAS
                     SNTVIQSIVDENMRGRVMSIYTLSFMGLLPFGNLLAGSV"
     assembly_gap    11773..11872
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11873..12063
                     /locus_tag="PL056_01870"
     CDS             <11873..12063
                     /locus_tag="PL056_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPM45793.1"
                     /translation="TLSFMGLLPFGNLLAGSVAQKVGTPATLFINGSLCIGASLLFMS
                     KTKMIEPLLKGIYDRKGV"
     gene            complement(12042..12923)
                     /locus_tag="PL056_01875"
     CDS             complement(12042..12923)
                     /locus_tag="PL056_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPM45794.1"
                     /translation="MRRFKRVYIEITNVCNMDCSFCPKTNRLKEFMNEEKFVHILDQI
                     QRYTEHIYFHIMGEPLLHSDLARFLDLSDEKGFKVNLTTNGTLIDKLADMLISKPALR
                     LVSISLHSFEGDKPALDSYLDTIFKFVLKAQKKNILCGLRLWNIKDSVENDENRHIIN
                     RIEKEFNVLSLQSELNSRGVEIYKGVYLNFAKQFDWPRLEKDLISEKGFCHGLRDQIG
                     ILVDGTVVPCCLDSEGSIALGNINSENFADIIASQRAESIYKGFSSRIAVEDLCRKCG
                     YRTRFAPKPSDQTPFLS"
     gene            13095..14303
                     /locus_tag="PL056_01880"
     CDS             13095..14303
                     /locus_tag="PL056_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012508794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPM45795.1"
                     /translation="MSFDSLNLIPQIKKALSDEGYTTPTPIQAEAIPLMLEGKDIFGI
                     AQTGTGKTAAFAIPILQILHSQSKHNNRRVIKTLICTPTRELAVQIGESFSAYGRHTG
                     IRNAVVFGGVGQLPQTRALERGVDVLIATPGRLLDLINQGFIDLKHLQIFVLDEADRM
                     LDMGFIHDIKRIIHEIPEKRQSVCFSATMPPEILRLAEKMLKDPVKVEVTPQATTVEI
                     IEQSVYYVDKINKRPLLAHILKNAEVTSALVFTRTKHGADRVVKELKQYNIPASAIHG
                     NKTQNIRQKALNDFKQGKIRVLVATDIAARGIDIEELSLVVNYDLPNIPETYVHRIGR
                     TGRAGAGGIAISFCDIEERMLLKDIQKLIGRKIPVVSDHPFVATEPVIEQQHIKKAPA
                     RLYRKSFRKY"
     gene            complement(14372..14677)
                     /locus_tag="PL056_01885"
     CDS             complement(14372..14677)
                     /locus_tag="PL056_01885"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPM45796.1"
                     /translation="MAKKKNSLVATTKTNFIKALKENVAATLTNDQAKEIYNVFTSIL
                     KDTIAAERKINLNGVGTFKVNNRKARKGINPKTRQAIKIPASKTVGFKPAPSFKKEL"
     gene            complement(14919..16115)
                     /locus_tag="PL056_01890"
     CDS             complement(14919..16115)
                     /locus_tag="PL056_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45797.1"
                     /translation="MKIKRVDRIVSFKGFLIFMAIFVIYLLLSETALHVFDTRDVLND
                     FEYYASKTFAEEQWSDAEKFRRDIISIGQRGVLIRDSIVYIYKPAESETVNFNSLGFR
                     GKEVEPRIEGEIRIAVFGASRIWGNYLPDDKTIPALIEKNMSGSFPNRKITVFNLGIE
                     GSDLQRSVETAKMFQKVLDLDIAVFYYGFSDMNYAYLHGNIDWIPFNSDSPMDEETFK
                     RLVGIMFPKGWLQNRKIYNLIIHSAKGELAGTLLGESQEYVDIPDLQKKTAEEFHNIF
                     FNRIENAAQHLKDKGVETILILPPVPQTKEPLSDLEKEMTLYYEAILPGYDRYIKKCT
                     VNVQKIMNEKKKSFLFVDQSDIFDGDHETVFFDGMHLTPDASTATAAEISEILVRFLG
                     QNMVRN"
     gene            complement(16117..17505)
                     /locus_tag="PL056_01895"
     CDS             complement(16117..17505)
                     /locus_tag="PL056_01895"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HPM45798.1"
                     /translation="MVFSSPVFLYIFFPLVWLVHSVIPQKTKNGFLMLASICFYFYGE
                     GFFLVLMIFTISVTFFASLLITKHPKTAFWTAFAGIVINLSVLAVFKYANFFTVQINH
                     LISGFSIRIPEVRLHLPVGISFFTFQAVSCIVDIYRNPDAEKPRFIDTALYISFFPQL
                     IAGPILRYNNFIPQLKNRMVHYRSLLYGIKRFIYGLAKKVLIANTLAVPVDKIFIADP
                     GSLDVLTAWTGAFLFTLQIYFDFSGYSDMAIGLGRMFGLKIPENFNYPLISRSITEFW
                     RRWHISLSTWFRDYLYIPMGGNRKGVFRMYLALWTVFFVCGLWHGASWNFVLFGLFHG
                     SAMILEKGGRSIGLALPGFMKRPVTFFVVMLIFVVFRSDSFSHFLSFAEAMFNPSGFN
                     FPLYVTEMRTMAAWTAGMLFMFPLYRIFEKKLRKNSYANYFETVFLAVIFALSISHSS
                     MMFADPFIYFRF"
     gene            17642..19399
                     /gene="dnaG"
                     /locus_tag="PL056_01900"
     CDS             17642..19399
                     /gene="dnaG"
                     /locus_tag="PL056_01900"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HPM45799.1"
                     /translation="MAFSEVNRLVSERIPLDKIISKYVKLEPSSKGFKGLCPFHNEKT
                     PSFYVNTVENFFYCFGCGVSGNAITFLMLKDGYSFSDAIKKLGEEFNIPELIKENPEI
                     DSALEKERAAIFNANKVAAKIFIELLEENAEALKYLEERAVTNEIRKKFGIGFVGSGE
                     RFVELMKERGVDMNTMMRAGLIRLDSYSRPVSFFYNRIIIPIIHNKNVIGFGGRIFSG
                     NGPKYLNSPDTIVFNKGENIFGIDFVREGLKGFPFVVLCEGYFDVIAMHRRGFCTAVA
                     SLGTAITESHLNTLEKFNKPVVLFLDGDEAGRKAAKRIAKLKIPEKIDLRAAFIKQEG
                     EDPDSLLVKDDGDRIVKDLIELSRPLFQELIDEQIRIYNSLDNLEEKIKVEDRIREIV
                     KNIPQRKLRTYHQYILKSSGNSIKIFYDRDKRIAGQLKKAEDGKIVKNEFLSKDRNDR
                     LRRLLVMAAIYNEFVPALETIKDVFFSSDFAVVYEDIMKKYFEGEDLMVISEKLDPEE
                     VINAELDNKQLSFIERTFRRELSYLRVQKNSELIKILETDESLESQQKISTLIMENRS
                     LKEIMVNNEIKEPDKSEEC"
     gene            19400..>19610
                     /locus_tag="PL056_01905"
     CDS             19400..>19610
                     /locus_tag="PL056_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45800.1"
                     /translation="MRKLPFMMRKKEPVPRKTDSRVLIKKMKDDEQDEKISNKIEQFQ
                     KEGKKPSVSLKNIAEGVLNSEGNVSI"
BASE COUNT         5703 a         4163 c         3522 g         6022 t
ORIGIN      
        1 gtgaaattga tcaggcacag cggctctatc agataagccg ttttaacacc gcggctctcc
       61 ctttctatgc gataatagat ccttcaacag ataccgttct tgcagtgcat cctgacatga
      121 caaatgatga aaataaattc gctgaattcc tgaaaaacgg aatagcatct ttcaatccgg
      181 cagaaaatgt cgttgaagaa aaaaaggaaa atacggggac agatttcact tttaaaacta
      241 tcgacggagc aaaaagtgtt tcgctctctt cattcagcgg aaagtgggtg cttcttaatt
      301 tctgggcatc gtggtgcgcc ccctgcagag aggaacttga acatgttttc cctgaagttc
      361 tgaaatcata tccggatgta ttgtttatga cggtggcttt tgacggagaa cagtcggcac
      421 atgccgcaaa aagctttatc gacggaatta gaatcccagg cattgtcaac cttcttggac
      481 ctgaagaccc gacatcggca ggactgtctg aagaatacaa atttgaagga tctcttccct
      541 cgacatatct catcagccct gaaggcagga tcgtctggat gaaatcagat gttgttgacg
      601 gaaaaaccct acgcaaagaa ctcgaaaaaa caagataagt aagggcggga tttatcacgc
      661 ccctttttca cgacataatc tttttctgaa tttttttcct gttgatatcc ttttcgacaa
      721 agacattgtg tcctgtgtaa ggatcagttt ctgtgtggta gatgagcgtt gatatggttg
      781 acggagtcgg cgtaaatatc tgaatttgct caggtttcat tttgagttcg cgtcttgaga
      841 aatcttttaa tgattccatg tccttatttg tacatcccgg atgggcggca atgaaataat
      901 atgtgagaaa ctgcttttta tctgcgactc tattgagttc attgaaaagc tctcggaatc
      961 tccgtaagtg cttctgagac ggcttgccca taagagaaag cactgtgttc tcaatatgtt
     1021 cgggtgcaat tttcatctgt cctgaaatat gtttttcaac aagttccttc aaataagcag
     1081 ttccatgttt tttatcggca aggacaagat catgccgcac accgcttgcg ataaaaactt
     1141 tcttcactcc gggaatattt gatatccttt tgagcagtct tatctgtcta ctgtggtcga
     1201 ttttcatatt ctggcatatt tccggatgaa ggcaccgttt ctttctgcac ggaccttctt
     1261 tcattttttt ttcacattcg aaaccgtaca tatttgcagt aggaccgccg agatcataaa
     1321 taattccggt gaatttcttt tttgtaagaa accttttaat ctcacgctct attgaatctt
     1381 cacttcttga aataactgtt ctcccctgat gcattgcaat ggcacagaat gaacattcgc
     1441 cgtaacagcc gcggtgtgtt gtcactgaaa attttattgt ttcaagcgcc cttatctcac
     1501 ctctttccct acattttgga tgggcatcat attcataatc aagttcatag attgaatcaa
     1561 gctcaattga agtcggcaag ggctgtggcg gattgtgtat caaccaccgt tcacctactt
     1621 tctggcacat tcccgatgca gtcaaagggt cgttattgcg atatgcagtc atgaacatat
     1681 ctgaaaatgt ctttttgttt tcaacaactt tttcaaacgg cggaagctga acaaatcctt
     1741 caaccggctc ttttgaaatg acgcataacc cttttatgga atcataatct ttttcgtttt
     1801 ttattgaatc tgcaagctcc agaatcgctt tttcacccat tccgtaaaca aggatatcgg
     1861 cctttgaatc aaacagaagc ggtcttctga cagcgtcatc ccagtaatca taatgggcga
     1921 tgcgccttaa acttgcttca attcctccaa gtacgatcgg cacagtattt ttgaagaatc
     1981 tgcggatgag accggtataa actatcagag cgcgatccgg acgacggtca ttcacaccgc
     2041 ccggagtaag atcatcatca ctccgtcttt ttttaagagc tgtataattt gaaaccatcg
     2101 aatcaacaca accggaagtc aaaccccaga aaaggagcgg ttctccaaga cgcatgatgt
     2161 cttcaccgct tttcatgtcg ggctgagaga taatccctac cctgtaaccc ttggcaatca
     2221 ggatgtgtcc gataatcgca atgccgctat atggcgaatc aatataggca tcacccgtca
     2281 caaggattat gtcgcactcg tcccagccga gcttttcaag ttcttcaact gttgcaggaa
     2341 taaacatcgt ccaccttaaa aacaactgtc agatggtaac tttttcttta cgcttttgca
     2401 aatatgaata acctttacat gcaattttga ggggttttat ggattttctt aaactcagca
     2461 accatcagaa agggatagtt cttcttttca taaacgcatt tctatggggg attcacggtc
     2521 ctgccggaag gtttctcgct ctgcgtggcg ttgatatgta ttttgtcgca ggaatgcgtc
     2581 ttctgattgg tgcggcggta ctttttctct ttcttgcggc aaggaaaggg ataacttttt
     2641 caataaaagg gcatgttaaa gatattctga tagtaagcat cgtggggctt tttcttaaca
     2701 ctttgtttta tcatcttgca ttgaagtgga tatcgggaac gctcgtgatg gtcattgaaa
     2761 acctgagtcc ggtgttcgtc atctttttca tctgggcgat atcaaagaca aaaccaaata
     2821 aaaatcagat acttgcgctc attctgtcat ttgccggaat gattgtgata gttgccggaa
     2881 aagataagct gtccgtcaac ggaattgact cacttcccgg aataatattt gggatacttg
     2941 cggggataac acttgcattt tattattttt tttccggaga actcatctca aaggtcaaag
     3001 acgaccccaa taaaatgttg tcacttttaa tgataatatt tctcatcgcg gcaatcccga
     3061 tgctccccct agtgatctca gctcaaaacc tcccttcaaa atttgatgaa tggttctggc
     3121 ttatcgaaat ggggatcttt cagagcggag ttgcctacat cacactcaat ctcgccatga
     3181 ggtatgtcag tgcttcaact gcaagcatat ttttcctttt cacaatattc ttcacgacaa
     3241 tgaatgaact catattcctt gatcttcaat taaatccata ccttatcatc ggctccatac
     3301 tcataattct cgctgcaaga aaggcatcgg tttaaaattt tgaggaaagt ctctgctatt
     3361 tttctacaag aacaggagtg ctttgagttc cgcttgtacc atcacctata ctgccgtaag
     3421 tgttgtcccc ccagcagtaa agttttttat cagctttaat gccacagtta tgaagtaatc
     3481 cagcggtaat ttcaatccag tcatcatcac ctacttttgt cggaacattt ttggctgtat
     3541 tttcttcatt gcctaactga ccgttctggt tgtcacccca gcaataaagt ttattatctg
     3601 attttatgcc gcaagtatga ttcatgccgc atgaaacctt tatccattca tcatcaccta
     3661 cttttgttgg atctgatttg tattcttctg ttccatctcc aagctcgccg aaattatttt
     3721 ctccccagca ataaagtttt ttgtcagttt ttataccgca gctatgaaaa tatccagcag
     3781 ttattgcaat ccatttgtct ttgcctattt gagtagggat aagctcattt tcaccccagc
     3841 agtagagtgt gttatctgac cttataccgc aggaataata atatccggca aataccgaaa
     3901 tccattcact gtcatcaaca ttcgtcggga cagttcttcc ttcttctgtt ccatcgccga
     3961 gacttccata tgaattatct ccccagcaat aaagtttttt gtcagttttt ataccgcaag
     4021 tatgatagac tccgcacgat acggaactcc atagatcttc accaattttc acaggaacag
     4081 ttttattgtt ctgctcttca atagttccat attcacttac ttttgtgatc gtatcatctc
     4141 cgatctgacc attaccgtta tatccccagc agtacagttt attgtcggtt tttaaagcac
     4201 agttgtgata atatccgttt tccacaactt tccatgaact gtcatctact ttaaccggga
     4261 aaggcttatt ataatcactg ctttcatcac ctatctgacc ataactgtta aatccccagc
     4321 aataaagctt actgtcagat cttattccgc aattgtctga gaaccctgca tttacctgaa
     4381 cccatgaatc atcatccacc ttcacaggaa cagttttttt aaaatcattc tccccgtcac
     4441 cgatctgacc gttgctgttc agcccccagc agtacagatt accgtcagac tttacacagc
     4501 aagtgtgttc acttcctgta gttataaaat tccatttatc atcgtttatt tttgtaggga
     4561 catatctttc atatccgttt ttcccgtcac ccgcctgtcc cgcattattc cctccccagc
     4621 agtaaagact tttgtcttca tgaatcccac aggtatgtga accgccggcg ttaactgaag
     4681 cccaaaactc attacttatt tttacaggta tgttcttatt attattccat aaaatggtca
     4741 actcctcacc atatacagtt tctgttccat cgccgatttt agctaatatg ttgtcacccc
     4801 agcagtatat tctgccatcg atgtttatgc cgcaggagtg agaatgacct gcagacaccg
     4861 atttccactt atcattaccg atttttgttg atataaacct gttttcattt gttccgtctc
     4921 caacctcacc gaaactgttt tttccccagc aatagagatt gttatcttta tttataccgc
     4981 aggtgtgcag acctcccgca gtcactgaaa tccatttatc atcatttagc ttagccggaa
     5041 tcaaggcatc cccggtcatt tcatctccga gtccaagttg cccaaaaaca tttaagcccc
     5101 aacaatataa agtatcgtct gtttttatag cacaagtatg ataatatcct gccgtcacgg
     5161 caatccattc atcatcgttc actttgaccg gaatgtttct gtcgttattt tcatcagatg
     5221 ttgttttctt cccgtcaccg atttcaccaa acctgtttga tccccagcag tacagttttt
     5281 tatccttttt tattccgcaa gtatgaaaag caccggctgc gatctcaagc cattcctcat
     5341 tatcaacttt aattggaact gtcttgtccg aacattctcc cggttcgttc ttatgattca
     5401 tacactcata cccgtctgtt ccgtcgccaa gctgcccgta actgttggcc ccccagcagt
     5461 aaagactttt attttcagaa tgtataccgc aagtgtgata atatcctgcc tctatcttaa
     5521 gccacagact attatcatta tcatcgtcga tttcttcatc attttcgttt tcgtcttcat
     5581 cttcattgtc cacatcatac gaatcagcat catcagattt ttcatcagtc atgtcggtat
     5641 actcagaatt atccgtgtca tctccgactt tgcctgagtt ttcagcattt tcacatgaaa
     5701 tgctcatcat taaaaaaaat gtaacgacaa tgactatcaa aaaatttctt ttcattatct
     5761 gtcctcacaa caaagattag attagtataa aatacacatt cttaagcatc gtgtcaatta
     5821 tcatcttttc cgcaaggaaa ggcatcggtt tagatttaga aattaatttc taatatgaag
     5881 tttttagcgg acacacatag caaaatgctt ttcactcttc attcctaagt caacacctcc
     5941 ggcaaacata tttatccgcc aggcagaaga attggcactt actgcagttg atgctgtcca
     6001 gtaatattca gaataaattc cgggaaaatt actcgcaggc tggtaagctc tgtcatagaa
     6061 gagcgtactg agctcgttga cattggggat tctccagtcc gaatatccgg catactcaag
     6121 attatcacaa taatttaaaa ctgtttccca tgttcctcct gaaacagctt cttttgccca
     6181 ttcaaagttc gtttttagat cttttacaat aattttatat tcaactgcga actcaacaaa
     6241 attactgagg taaatggcat tccctctcac acaccggaca taagtttcgt cagtttgatt
     6301 tcttgtacct actgttccaa ctccttgatt aacactccat ttgcgttttg aatccattac
     6361 aaatgaagta gtggtcaata tccattttac aggtgtgtct ataaataaat cagtatctat
     6421 tgaaggattt gattttccat agtctgtgat gctttctaat tcaattcttg atggcaacct
     6481 ccagtcatca taccctccgt aatccaagtc attgcagtaa ttaattgctt cctgccaact
     6541 acacattgat ccgactggat ctccgaaagc acacccggca tatgtttcgg gaattgttcg
     6601 ctgccacatc agaccagtat tattgtcggt aataatttca tcgccttctg agattgaaac
     6661 agcatagctt ctgggaatac atttttctat gtattgtgca tcctgaccgg aaaaattctc
     6721 gccagacaat ggacatggaa tttcctctgt attgttataa caacttattt gacccgtgca
     6781 aatagtcctg cctgttgatt caggtaaaat atctgcatca agatctgtgt tttcttcatc
     6841 ccgctcatca atactctcag agatatcttc ttcaaactgt tcgtcaaaat attcatcctg
     6901 tttttcatct tgaatgtcat caatttttgc gtcttcatca tcgttgaata attcataatt
     6961 atcatcaaca atgtctatat caacaacaag caagctgtca gaatcagtgt tgtttttatt
     7021 aattatttct gatttacacc ctgatgcgaa gattagtata aaaataaatg caaaaaaaac
     7081 tctcatgctt taactccata ataatgtcag ccaaaaaccg aatacattta tacacaatta
     7141 aaatactttt ttcaacattc cttgccgcga gaaaggcatc ggtttagata ttttaaaagt
     7201 tcacaacggc ctgtaatccg ccatattcag aagacacgac aggaataacg ctcagatttt
     7261 tgttcttgat tttatgaaga agagggatga aaagtccagt gagagagccc aaaactgctc
     7321 cggcaattac atctgtcgga taatgctctc ctgcggtgat cctgagtatt cctgcggcgg
     7381 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nctgtcattc attgaaacag
     7501 cactcctgtc aaaagcgttc agatcatatt tatccagagc aagaatttca cttttttcaa
     7561 aatctgtcga aggagtgaac atgaaagatg tcatcagtcc aagtcccgct gagaaaataa
     7621 atgcctcttt cgcatcagtc agctcaaaag gaaattcgtc actgtttttt tcaagagaca
     7681 gtgccgttaa tgtaaagaga aaaacggcaa tgaaaaccgt tattttattt gaacagtttg
     7741 actgcactta ctatgctccg ggtgacaagt tttcaagaag gaaatctata atgttctttc
     7801 gcactattcc gttgcggtca tctccccaga ttttgtccat tgaaagaacc attttgggat
     7861 agttatcatt tattttttca agcggtttaa attcccgttc ttctgtttcc tttgaagcaa
     7921 gaagatatgt cacctgaata taagtctttt ccccattgcg cgtggcaaca aaatcaattt
     7981 ccatatcatc aaattttcca gttgaaactt tgtatccgcg tcttaaaagc tccagataaa
     8041 ctatattttc cagaagtcct gcgatatcac cttttttata accgagaaca ctgtgacgga
     8101 ttccgatatc actcatatag tatttttcgt aaagctcaag atgctttttc cctttcaggt
     8161 catatcgccc tgatttatat gcaagataag catcttttaa gtagttgata taattagaaa
     8221 ctgtcccgac accggcattg atgtgctgcg atttcaaata atcagctatc tttttggcgg
     8281 ttgtaatact tccgcagtta tcaaaaacaa aagctgttat tttttccaga agagaaatgt
     8341 cccttatgtc atatcttgca acaatatctt ttaaaagaat cgtgttgtag atcgagttga
     8401 tatattggaa gaccacaaca tcatcaaatt taagattatg aattcctgga agcccgccat
     8461 atttaagata agtctcgaat tctatttcat ttttgctttg atcttccatc cctctaaatt
     8521 gcagaaattc tttaaaactt aaaggaaaca tttcaatttc aatatatctt ccagtcaaaa
     8581 gagtggcaaa ttcggaagaa aacattcgtg aatttgaacc agtcagataa atatcaaaaa
     8641 caccttctgc aagaatagag ttgacagctt tttcccatcc cggaatatcc tgaacctcgt
     8701 caatgaaaag gtatttctgc ttattattct tttgtatttt ctttttcaca tattcatata
     8761 acactctgta atcagttata aactcgaact ccataagttc catgcttata taaactatat
     8821 tcttttccgc agctccgttc cggattatcc ggttgatcaa catctttaaa aaagtgcttt
     8881 taccgcacct tctcaagccc gagacaacct ttatgatcgg cttttccata aaaggttcta
     8941 ttttttttaa atattgttct ctctcaaaca tgaacacctc caaaagtttc actcatacaa
     9001 gaatcttttc acattttact aaaaagtttc acttatacaa gaatcttttt gtgtttttgg
     9061 caaaagtttc acttatacag gaatctttca gctccttttc tgtctgaaaa tatcttcaag
     9121 tgttacgaaa tgattgaaat attcgctgtc tttaagggct tcatctggtc cataaagact
     9181 gatcagtgct ttttcgagtg tgtatccatt cggaattttt aagagtgcac actttttttc
     9241 aacttcaggg atgatctttg tagttatttt gccggtgtaa tgctttattt cgcaaactgt
     9301 gataactttg tccgctcttt tataaagcag atcaatttga aatttggaat cattctttcc
     9361 aaaatatggc gatgcataca gagtttcatc tgcaaaaccc attatttcag caagatacca
     9421 cgaatgtttc aagcagaatc tttcaaaagc aaagccgaac catttatcaa gactgtcggc
     9481 agaaatcttt tcaaaaagtt tagttgtgtt tttctgattt tggatagccg tcatattcgg
     9541 ttccatatac ttgaaataaa acctcaaaaa ctcatctgca agacggtatt tcctgaattt
     9601 tgtgttccag ccctgctcaa aagggacaaa tgattcaata agttcagctt tttcaagttg
     9661 taaaagaact tttttaagac tgcctccact ttttttcgca attttctcag taatttcctt
     9721 aaatgaaaaa gctccgttct ttaataaatt gacaacatca agatatgttt cagctttaga
     9781 atattgtttg taaaatattt tctcgacttc attaagcatt aaactgtttg gagaaaagca
     9841 gagacggtta atatttttat aaaatgaagc ggatatgtca atctcttcaa aatattttgg
     9901 aactccgccg aagataagct gatagttcag aatttcatac ttatctcttt ttcctttaaa
     9961 aaatgaggct atttcgtcgg gattcaaccc ttttatcagc atttcttctg taactctgcc
    10021 gtaaagagcg gtggagttta taaccttatc aaccataaat gaagcaatcg acccgcataa
    10081 gatcagcatg acattctgat ctttccagtg attatcccag aaatacttta ttatacttat
    10141 caactgcttt cttcctgaag ccatccattg tatctcgtca aagaaaacaa ccattttttc
    10201 tttattattt ttacgggaaa ccattttttc tgtgaaatat ttgaaaactt tttcccagcc
    10261 ggagaatttc atgtcttcca gaagagaatc ttttgtaaat tcagccattc tatctttgaa
    10321 atattccatt tgcttagccg aactacttcc ttcaatacct tcaaaaatga aatggcatgg
    10381 cttgtctttt aaaaattgtt ttatcaatgt acttttcccg acacgccgtc ttccatatat
    10441 gacaacaagc tcacttttct ttgactggaa atttttctct aattttttga gctcacttaa
    10501 cctgccgaaa aacataagct tttcctccat caaaaaaatc cgccttcaaa acaatttaca
    10561 gattgacggc ggattatgca aattttctta agggtttcta tttgctttta ttaattttcc
    10621 tgatattact tttttaagat ttttggagaa aactatgtct tttagcggta tcagaaacat
    10681 tttcagggct ttaagctata aaaactataa gatctatttt ttcgggcaga tcacttccca
    10741 ggtcggttta tggatgcagc aggtcgctct cgggtggctg gtgtaccgtc tcacaaattc
    10801 agcttttctt ctgggtattg taggattttc tgcacagatc ccgtcattta tcctttctcc
    10861 aattgccgga atagccgctg acagatatga ccggtataag atactgataa tcactcagtc
    10921 ccttttcatg gtgcaggcac ttgttcttgc cgcacttgtt ctgcttgata ttgcaggcgt
    10981 tacaaccatt ataatcctta gcttgatcca gggcataatc atggctttcg atgcaccggc
    11041 aagacatgct ttcgttgttc agatagttga cagaaaagat gatatcggca atgcaatcgc
    11101 ccttaactcc accacttttc atgcttcccg tcttgtcgga ccaccgcttg ccggagtaat
    11161 aatcgccgtc gcaaatgaag gaatatgttt cctcatcaac ggattgagct atatcatggt
    11221 cattgccgcc ttgtttatga taaaagtaaa acctctggaa cataccggaa ccagcaagag
    11281 tgcaataaat gacatccgtg acggatttat ttatgcattc agatcagttc cgataaaagc
    11341 aatactcctg atggtcgctt ttgttagcat tttcggaatg ccttacaccg ttcttcttcc
    11401 tgtaatggca cgcgatgttc ttcacggaac ttcacagact ttcggaattc tgatgggagg
    11461 agccggagtc ggagcactga ccggagcact gtatctcgca tcgaaaaaga acgcccacgg
    11521 actttttaaa aaccttgcaa tagctccgtt gattttcggt cttggaatga tcggcttttc
    11581 aatgtcaaga agtgtcgaac tctctttgat cctgcttgtg acaggcggat tcggcatgat
    11641 gaccacaatg gcttcatcaa atacagtcat ccagagtatc gttgatgaaa atatgagagg
    11701 gagagtgatg agcatttata cactatcatt catgggactt cttcctttcg ggaatctgct
    11761 tgccggctca gtnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnatacacta
    11881 tcattcatgg gacttcttcc tttcgggaat ctgcttgccg gctcagtagc tcaaaaagta
    11941 gggacccccg cgacattatt tatcaatgga tctttatgca tcggcgcatc acttcttttc
    12001 atgtcaaaaa ctaaaatgat agaaccgctg ctgaaaggaa tttatgacag gaaaggcgtt
    12061 tgatcagaag gtttcggggc aaatcttgtc cggtaaccgc actttctgca aagatcttcg
    12121 actgcaatcc gggaactgaa acccttgtaa attgattcag ccctttgact tgcgattatg
    12181 tctgcaaaat tttctgaatt gatattacct aaagcgatgc tcccttctga gtcaagacag
    12241 cacggcacaa ccgttccatc tacaagaatt ccgatctgat cccgaagccc gtggcagaat
    12301 cctttttcac tgatgagatc tttttcaaga cggggccagt caaactgttt tgcaaaatta
    12361 agatagaccc ctttataaat ttcaacaccc cttgaattca gctctgactg aagagataag
    12421 acattaaatt ctttctcaat tctgttaata atatgtctgt tttcatcatt ttcaacggaa
    12481 tctttgatgt tccagagtcg aagtccgcag agaatatttt ttttctgagc ttttaaaaca
    12541 aacttaaata tcgtatcaag ataactgtca agagccggtt tatctccttc aaaactgtgc
    12601 agagaaatgc tcacaagccg caatgccggc tttgaaataa gcatatctgc caatttatca
    12661 atgagagtcc cgtttgtcgt cagattaact ttgaaccctt tttcatctga tagatcaagg
    12721 aaccgtgcca gatcagaatg gagaagcggc tcccccataa tgtggaaata gatatgttct
    12781 gtatatctct gaatttgatc aagaatatgg acaaacttct cttcattcat gaactctttc
    12841 aatctattgg tctttggaca gaaagaacaa tccatattgc agacatttgt aatttcgatg
    12901 taaacccttt taaaccttct caaaacgatc accggtaaaa atctctttgc tgaatttagt
    12961 caaaaactga tccttgacaa gattaagatt gatttttcta aattagtgtg atacatagcg
    13021 gtgcgtattt ggtctgctcc gcaaaatacc gaaacattat tattaatttc cgtttggaga
    13081 gcttgatgac tctgatctca tttgacagct taaatttaat tccgcagata aaaaaagccc
    13141 tttccgatga aggatacacc actcctacac cgatacaggc cgaagccatt cccttaatgc
    13201 ttgaaggaaa ggacattttc ggaatagccc agaccggcac tggtaaaacc gccgcttttg
    13261 caattcccat attacagata ctccactcac agagcaagca taacaaccgc agagtgataa
    13321 aaacgctcat ctgcactccg acccgtgaac ttgctgttca gatcggggaa agcttttctg
    13381 cttacggtcg tcacaccgga atcaggaatg cagtggtatt tggcggtgta ggtcaacttc
    13441 ctcagaccag agcactggag agaggcgtcg atgttctaat tgcaaccccg ggaagacttc
    13501 ttgacctcat caatcagggt tttatagacc tgaaacactt acagatattt gttcttgatg
    13561 aagccgaccg tatgcttgac atgggtttta tccatgacat aaaaaggatc attcacgaaa
    13621 ttcctgaaaa aagacagtct gtctgttttt cggcaacaat gcctcctgaa attctgcgac
    13681 ttgcggaaaa aatgttgaaa gatccggtaa aagttgaagt aacgcctcag gcgacaaccg
    13741 ttgaaataat agagcagtcg gtctattatg tggacaagat aaataaaagg cctcttcttg
    13801 cacacatttt aaaaaacgct gaagttacat cggctcttgt ctttacccgc acaaaacacg
    13861 gtgcagaccg agttgttaaa gaactgaaac agtataatat tccggccagt gcaatacatg
    13921 gcaacaaaac tcagaatata agacagaaag cgcttaacga tttcaagcag ggaaagataa
    13981 gagttcttgt cgcaactgac attgccgcgc ggggaattga tatcgaagag ctttctcttg
    14041 ttgtgaacta tgaccttccg aacattcctg aaacctatgt ccaccgtata ggaagaacag
    14101 gaagagccgg tgcaggcggt attgcgatat cattctgcga cattgaagag aggatgctgc
    14161 tcaaagatat tcagaaactt atcggcagaa agatccccgt cgtgagcgat catccgtttg
    14221 ttgcaactga acctgtcatc gaacagcagc atatcaaaaa agctccagcc cgtctatacc
    14281 gcaaatcttt cagaaaatac tgaacatcat caacaggcaa aaaaaaaccc ttcccggagg
    14341 aagggtctga aaaattttca ggaaataact attacagttc tttcttgaaa gaaggtgccg
    14401 gcttgaatcc aactgtcttt gaagcgggga tcttgattgc ctggcgagtt ttcggattga
    14461 tacctttgcg agcttttctg ttgttaactt taaaagttcc aacgccgtta aggttgatct
    14521 ttctttctgc tgcaattgtg tccttaagaa ttgatgtgaa cacattgtag atctcttttg
    14581 cctggtcatt ggtaagtgtt gcggcaacat tttccttcaa tgccttgatg aagtttgttt
    14641 ttgttgttgc aacgagtgaa tttttctttt ttgccattcg aaactccttt tctcaaaagt
    14701 tcataaaagg gactcgctta ttctatgata ctgaaaagca atgtcaactc tttttgatgt
    14761 tttttttaac ttttttttca accttagcca agccagcaaa gggtttggca gacatggcga
    14821 gcatttttat cagcaatttc aaatagttac tggcggtata acatcttgtt tgcaaattga
    14881 ttcaacctta atatttttag ctacttaaag gttttttttt aatttctgac catgttttgg
    14941 cccaaaaacc tgacaagaat ttcagaaatt tcagcggccg ttgctgtcga ggcatccggt
    15001 gttaaatgca tgccgtcaaa aaaaactgtc tcatgatcgc cgtcaaaaat atcggactga
    15061 tcaacaaaaa gaaagctctt ctttttctca ttcattatct tttgaacatt cactgtacac
    15121 tttttaatat atctgtcata tcccgggaga atggcttcat aataaagcgt catttctttt
    15181 tccagatcgc ttagcggttc tttggtctga ggaacaggag gaagtataag aattgtttca
    15241 acgcccttat cttttaaatg ctgagcagca ttttctattc tgttaaaaaa tatgttgtga
    15301 aactcttccg cagttttttt ctgcagatcc ggaatatcga catattcctg actctctccc
    15361 agcaatgtgc cggccaactc cccttttgct gaatgaatga tcagattata tatctttctg
    15421 ttctgcagcc atcctttcgg aaacatgata ccgacaagcc gcttaaaagt ttcctcatcc
    15481 atagggctgt cggaattaaa cgggatccag tctatgttgc cgtgcagata tgcatagttc
    15541 atatccgaaa aaccgtagta gaaaacagct atgtcaagat caagcacctt ctgaaacatc
    15601 tttgccgttt caacactcct ttgaaggtca cttccttcaa ttcccagatt gaaaacagtg
    15661 atcttgcggt ttggaaaact tccgctcatg tttttttcaa tcagagcagg gatcgttttg
    15721 tcgtcaggga gatagttacc ccagatcctt gacgcaccga aaacagctat tctgatctca
    15781 ccttcgatcc tcggttcaac ttcttttccc ctgaaaccta gagaattgaa attaaccgtt
    15841 tcgctttctg ccggtttata gatgtacact atgctgtctc ttatcagaac tcctctctga
    15901 cctatgctga taatgtcacg cctgaatttt tcagcatcac tccattgttc ttcagcaaag
    15961 gtttttgaag cataatattc aaaatcattc aggacatctc tggtatcaaa aacatgaaga
    16021 gccgtttcag aaagaaggag atatatcaca aaaattgcca tgaatattaa aaatccttta
    16081 aatgatacga tcctgtcaac tctttttatt ttcatgtcag aacctgaaat aaatgaaagg
    16141 atctgcaaac atcattgaac tgtgtgagat cgaaagagca aaaataactg ccagaaaaac
    16201 tgtttcaaaa tagtttgcat acgaattttt ccgcaacttt ttttcaaaga tccggtacag
    16261 cggaaacata aacagcatcc ctgcagtcca ggcggccatt gttctcatct cagttacata
    16321 caacggaaaa ttaaatcctg aagggttgaa cattgcttcg gcaaatgaca aaaaatgaga
    16381 aaaagaatca gacctgaaca caacaaaaat aagcattaca acaaagaaag tgacgggtct
    16441 tttcataaac cccggcagtg ccagtcctat cgaccttcct cctttttcca gaatcatggc
    16501 cgatccgtgg aaaagcccga acaacacaaa attccaagat gcaccgtgcc agagtccgca
    16561 tacaaaaaaa actgtccata gtgccagata cattctgaac accccttttc tgttaccgcc
    16621 catcggaatg taaagataat ccctgaacca tgtggaaagc gaaatatgcc accttctcca
    16681 gaactcagtt attgaccttg agataagcgg ataattgaag ttttccggga tcttaagccc
    16741 gaacattctg ccaagtccga tcgccatatc ggagtatccc gaaaaatcaa aatatatctg
    16801 cagtgtaaag agaaatgctc ctgtccatgc tgtcagaaca tccaacgacc caggatcagc
    16861 aataaatatt ttatccacag gaacagctaa agtattagca ataaggactt ttttcgccag
    16921 accgtaaatg aaccttttaa taccgtacaa cagtgatctg taatgcacca tcctgttttt
    16981 aagctgagga atgaaattgt tatatctcag tatcggacct gcgataagtt gcggaaagaa
    17041 ggaaatataa agagccgtat caatgaaacg gggcttttcg gcgtccggat tccgatatat
    17101 atcaactatg caggataccg cctggaaagt aaagaacgag atgcccaccg ggagatgaag
    17161 ccggacctca gggatcctga tactgaagcc ggagatcaga tgatttatct ggacagtaaa
    17221 gaagttcgca tatttgaata cagcaagaac gctcaggttt atcacgatcc ctgcaaatgc
    17281 cgtccagaaa gctgttttag ggtgttttgt aattaaaagc gaggcaaaga aagtcaccga
    17341 tatcgtaaag atcattaaaa caagaaaaaa tccttccccg taaaagtaaa agcagatgct
    17401 tgcaagcatt aaaaaaccgt ttttcgtttt ttgaggaatg accgaatgaa ccagccatac
    17461 aagcggaaaa aaaatgtata aaaaaaccgg actgctgaaa accataacat ctcccggaac
    17521 attcttacgg cattatcccg aatcaaatgg aaaaatcaac atcattatat ttaaataaaa
    17581 gtttgattta tagccatctt tatgtatcat tgcgcaagaa attttttggg gttttcctga
    17641 gttggcattt tcagaagtca acaggctcgt ttccgagcgc attcctcttg acaaaataat
    17701 ctcgaagtat gtgaagctgg aaccctcttc aaaaggattc aaaggactct gcccttttca
    17761 caacgaaaaa accccttcat tctatgtcaa cactgttgaa aacttctttt actgtttcgg
    17821 ttgcggagta tccggaaatg cgataacatt tcttatgctc aaagacgggt attcattttc
    17881 cgatgcaata aagaaactcg gtgaagagtt caatattcca gaattaataa aggaaaatcc
    17941 tgaaatagac agcgctcttg aaaaagaaag agctgctatt ttcaatgcaa acaaggttgc
    18001 cgcaaaaata ttcattgagc ttcttgaaga aaatgctgaa gcgttgaaat atcttgaaga
    18061 gcgggctgta actaacgaga tccggaaaaa gttcggaata ggttttgtcg gttcgggaga
    18121 acggttcgtt gagttgatga aagaacgggg cgtcgatatg aacacgatga tgcgtgcagg
    18181 actgataaga ctcgacagct actccagacc ggtcagcttc ttttacaaca ggatcatcat
    18241 ccccatcatt cacaacaaga atgtcatcgg tttcggcgga agaatatttt caggtaacgg
    18301 ccctaaatat ctcaattctc cagatacgat cgttttcaac aagggagaaa acatcttcgg
    18361 aatagatttt gtaagagaag ggctgaaagg ttttcctttt gtggttcttt gcgaaggtta
    18421 ttttgatgta atcgcaatgc accgcagggg attctgtaca gccgttgcat cgcttgggac
    18481 agcaataacc gaatctcacc ttaacacact cgagaaattc aataaaccgg tcgttctttt
    18541 tcttgacggt gacgaggcag gaagaaaagc ggcaaaaaga attgctaagc tgaaaatacc
    18601 tgaaaagatc gatctccgtg cagcatttat taagcaggaa ggcgaagacc ctgattctct
    18661 tttggtgaaa gatgatgggg accgtattgt aaaagatctc atcgaactgt ccagaccgct
    18721 ttttcaggaa ttgatcgatg aacagatcag aatctacaat tctcttgata atcttgaaga
    18781 gaaaattaaa gttgaagaca ggataaggga gatcgtcaaa aacattcctc aaagaaaact
    18841 aaggacttat caccaatata ttcttaaaag cagtggcaat tcaataaaaa tcttctatga
    18901 cagagacaaa agaattgcag gacagcttaa aaaggccgag gacggaaaga ttgtaaaaaa
    18961 cgaattcctc tcaaaagaca gaaatgaccg gcttagacgt cttcttgtca tggctgcaat
    19021 ttacaatgaa tttgttcctg ctcttgaaac cataaaagat gtgttctttt cttctgattt
    19081 tgctgttgta tacgaagata ttatgaaaaa atactttgaa ggtgaagatt tgatggtgat
    19141 ttcggaaaaa cttgatcctg aggaagttat aaacgctgaa ctcgacaaca aacagttgag
    19201 ttttattgag cgtacttttc gcagggaact ttcatatctg agggttcaga agaacagtga
    19261 gttgataaaa attctcgaaa cagatgaatc acttgaaagt cagcagaaaa tatccacgct
    19321 tatcatggaa aacagatcgt tgaaagaaat aatggtcaac aatgaaataa aagaacctga
    19381 taaatctgag gagtgctaaa tgagaaaact gccctttatg atgagaaaga aagagcctgt
    19441 tccaagaaaa actgactcca gagttctgat caaaaaaatg aaagatgacg aacaggatga
    19501 aaaaatttcc aacaagattg aacagttcca gaaagaaggg aaaaaaccga gtgtatcttt
    19561 gaaaaacatt gccgaagggg tgcttaattc tgaaggcaat gtttccattc
//
LOCUS       DAOOYB010000017        19468 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_2684_length_19468_cov_8.334158, whole genome shotgun sequence.
ACCESSION   DAOOYB010000017 DAOOYB010000000
VERSION     DAOOYB010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 19468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19468
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2684_length_19468_cov_8.334158"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="PL056_01910"
     CDS             <1..717
                     /locus_tag="PL056_01910"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HPM45801.1"
                     /translation="KGVESILNPYCEYALDMAVALKKRLKDVKITAFTMGPDSAELVL
                     RRALSLGADEAVLISDRAFSGSDCWATAYTLSKGIRHQLKDPDLIICGKQAIDGDTAQ
                     VPGEISEILNMPLLPYVTEIVDINEIISVKCEMGDNILELSSKLPVVITVGNGSNIRS
                     IPSVKDAAAAFSANIVHLDNTIIKCDLTQTGLKGSPTRVVKIEPVTANGKCEFYNEEN
                     LDKGLNKLFDLISIVKGDKV"
     gene            714..1817
                     /locus_tag="PL056_01915"
     CDS             714..1817
                     /locus_tag="PL056_01915"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012082686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPM45802.1"
                     /translation="MRKIEFIDLKAQYAAYKNEIDNAMEKVCLESSFIMGQQVGELEK
                     RLSEFTGSPHAISCSSGTDALLLALMAIGVKDGDEVITTPFTFIATAEVISLLGAKPV
                     FADIDETTYNLDPGKIEEKITERTKAIVPVCLYGQVAEMDEINAIAAKHGIFVIEDAC
                     QSFGAEYKGKKSCNLSKLSCTSFFPSKPLGCYGDGGAVFTSDAELAEKLKSIRVHGQT
                     QRYYHKYIGINGRLDTIQAAILNVKMDHLEEELKKRVEVADHYSKNLAGNPGIVLPEI
                     KSDRTSVYAQYSIRVKDRDGFTAKLNSAGIPTAVHYPRPLHLQEAYSYLGLKNGDFPV
                     SEKVSNEILSIPMSPFLKKDDQDYIIENIIKNV"
     gene            1828..2907
                     /locus_tag="PL056_01920"
     CDS             1828..2907
                     /locus_tag="PL056_01920"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPM45803.1"
                     /translation="MKIGLVSNMYPSENDKNYGVFVKGLVDSMSKKGVEFSFTAVIKG
                     KQNGRWQKIWAYLKLYFSAVFNLLFKECDIVFLHYPLHLAPLIYMMNIFMIKPLVVNF
                     HGTDMNPQSKGLQVLENILKRSLDNVKMVVVPSSFLKKEVMKKYGVPEEKIFVSPSGG
                     VDFDKFRSFDDNPAAVKPYLAGYVSRLTKEKGCDVFLESVISLKREGSVEGKKFLIAG
                     TGPMQNEISDTIRKEGLQETVEFKNINDQSMLPQFFNDMELFVFPSLSESLGLVGLEA
                     MACGTPVTGSEIGGISDYLTDGQNGYFFKAGDPSDLAQKIRQFMLLTRIEKMRFKENA
                     KNTATQYERERVVSMLIRMLELLLF"
     gene            3016..4572
                     /locus_tag="PL056_01925"
     CDS             3016..4572
                     /locus_tag="PL056_01925"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HPM45804.1"
                     /translation="MEKELGKEKNEISLKDVYLVLVNNKKLYLIFSILTFCSLAVIFL
                     FFLTPVYEVNASLEIRQQNASPLSINPMAAMLIGGTAMSDKTIDYEVLRSRRILNSII
                     ENKGLQMNITKKWNTMFSYFLGLLFKNLPSDSFVYFKKIPDVLKSESGKIDAKEEGYS
                     IYFNDEKAECLWDKECSFMGSAVQLDKIGTFSAEMTFKFEYEQIVDARDRVNGWINIG
                     KAEESEMILLGLVHESPTMGSLILNDLVNAYVNIKSDWEKEDITAKKKYINSTLDELS
                     VSLDEKSQKMILFQQTEKTIMPEVEIPELLKKQETLKIQIEELKFKKKIIAGAMENIG
                     KSLDTPISVPFAFEDLSTQEALKSYNDLLFRKSRLSQRVTEDHPMLSAIEKEIETNTD
                     SLKKMLKETADQYDKGINILNELLSMISSGQHKIPENLFSYLRLKRDVELAEKVFVTL
                     SAKLYESAIEPNVGIAPVRLIDLPDPFVKRYFPKAKIFAVIIFIITLFSGAVAIFIKE
                     FLKAVLKSIK"
     gene            4583..6277
                     /locus_tag="PL056_01930"
     CDS             4583..6277
                     /locus_tag="PL056_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HPM45805.1"
                     /translation="MKISKTIIGGAIGNCVHVAGVFSYIQIAEQNGYKGVFLGSAVDP
                     DLFASKIKELSPDIVCISYRLSASAIYPILKRFFDSLGSLEKCAGMLFYFGGTPETIE
                     IAKKFPRFSRFFQGEEPLEIIYSSLNIKNDIEIIALRGPESSAKVPVMNPSLKNRLSQ
                     KTYFPMIRHHFGLPTMKETLEGIRQIADSGVVDVISIATDQNAQEFFFSPEQMNPLLD
                     GAGGVPVRTESDLFQIYDAAGRGNFPLLRIYSGTRDLLRWAEMSLRTINNAWGAIPLF
                     WYSQLDGRSKRSVAEAISEHQEAIKWYADHGIPVEINDSHHWSLRDAPDVMAVVDAYI
                     SAYNAKKLGVKIYIAQFMFNTPRITSGRMDLAKMLAKDTLISELVDDDFQYIKQVRAG
                     LTHFSIDQSVAKGQLAASTVLSMAIKPHILHVVSFSEADHAAKPEDVIESVKIAKGVL
                     KNCIYEFPDLSNDPIVQERKNNLIQEARKVLAVMIETGSCYDDPLISSEYLATAVLSG
                     FLDAPHLKGNPNAKGSIRTMPVNGGYEIVDENNKVISASEYVSRIIEFRKKGGSIG"
     gene            6270..7358
                     /locus_tag="PL056_01935"
     CDS             6270..7358
                     /locus_tag="PL056_01935"
                     /inference="COORDINATES: protein motif:HMM:NF014381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HPM45806.1"
                     /translation="MVNPAPVTIIGAGNSGLAMAAHLSLNGHPVCLWNRSLKTLENII
                     SEGQGSILAEGVVNGNAKIDLITGDIEKAVNFSRIIMVTTPADAHSNISMLSAPYLKK
                     DHIVILNPGRCFGAIDFLKKLSDKNCKDLPTVAETQTILYTCRKMPPNRSLILALKKK
                     VLISSTNFKNTMKVMENLPDCIRPYFHPAGSLLETSLGNVGMILHCLPVLMNVGWIEN
                     KKTSFKYYYDGITPSIAKLLEKIDNERLDVARHLGLTVPSLSAWLEVTYGVSGESLYD
                     KIQGVESYRTIEALDTLEHRYLLEDIPFGLVPIEDIGKQIGLTMKITTLIIELANEIF
                     GFDFRDKGRTLKSLGISLSDLILWNKNE"
     gene            7351..8553
                     /locus_tag="PL056_01940"
     CDS             7351..8553
                     /locus_tag="PL056_01940"
                     /inference="COORDINATES: protein motif:HMM:NF026968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HPM45807.1"
                     /translation="MNKYKNHAVICGSYVNALGLARSLGAEGIAVTVIDYTKNISFYS
                     KYVHGIVCPKPDEDEEGFISFLIDLGHRFDEKPVIFVTNDIWLIPVSRNGERLSKYYH
                     IPMSDWEIIKQCSDKTYMYKIAEENNIPYPKTLFLEKSSQFLKKEHSLKYPVILKPSV
                     TIGFSEKLKSYGRSLEFKDQSSAENWIDKIRNAQLDDVPVVVQEMIPGGAENLFTFTS
                     YSRKGGETVAYSTGHKIRQSPPHAGTIISGHVIPNKDVIENGINVIKKMKFDGIANTE
                     FKYDHRDGLYKLIEINPRPGMWNFSVMASGINLPLYAYLDSLGIDYKPVKSSDKELNW
                     VFWSSDLYYSLFGFKKMGFPEYSLTLCSWWKSLKGKKVSAVFSLMDIKPALFLFSSFF
                     YSKLKRFF"
     gene            8553..10028
                     /locus_tag="PL056_01945"
     CDS             8553..10028
                     /locus_tag="PL056_01945"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HPM45808.1"
                     /translation="MSSKLAQSAISTSFIWNFGAVVFRRIINVIAIPVIARLVSKEDY
                     GNIQIIISTFMLLAVIRNSGFNDYFIKEADDRDKKFFSLFVNTMYLNTFVIAVIFIPA
                     VGIYSYFNDVSSIYTYLVLIVVLLFINVGEIAGETYFRQKVCFKNIAIAGSIKILSYP
                     IFTIIFLLIRPDLFSLFAAFALSIVAEIISYFFFLPDNKVFLPDIKKMTEGFLLQFKE
                     RVFCFSVSAGMFISNFNVSFPIYLFAYAFSAEHSAQYAISAQLVSIPFSLIIDSLSKV
                     IYPVFIKQNEEERRRFYLSILKFFPFILYPMLLWIMIYAEGVVSLFLGEKWIDVVPII
                     RIVSIPVFFNVLSSPLGPVATALGKPHYRVLWSGGSVLLALLAGSMGLQFGFLWGVAS
                     VISSRVVTLIVWQVVACRMINLSHFKLAVQLIPYVPFWIMLVVFWIFFPEVSSFPYVR
                     MFVSGTVGMLIYFLLFMLFFKNDVMQIKKLVNDLFKKKVFE"
     gene            10025..11428
                     /locus_tag="PL056_01950"
     CDS             10025..11428
                     /locus_tag="PL056_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPM45809.1"
                     /translation="MNFKVTVPDNFFAERQYIANVMLKEFLGLDYDIEQVADAKTVKI
                     SNNENTREITFPDVFFSMDESDWLKERSLPKEPLKIINSSVFPGNPILSSDRIPLIYG
                     TNESEVSVSEEEISIPVDVFGSAFFMLTRYEEAVKNNRDTHDRFPASASLAFKEHFLC
                     RPIINEYVEILWSSMRVLWPEIERKERKFEMVLSHDVDRPFKYRNMLPATFLKIVAAD
                     LIRRRSVRKAYESVKSYIAVKMGEIDRDPFNTFDFIMDLSEKAGLKSCFYFISDHSDN
                     KINCDYSINDPEMISLMKKIHSRGHEIGLHPGYDTSDNPEKLKNEFVKLRKICSANGI
                     KQEKWGSRQHYLRFSVPQTWRYLSEAGIDYDSSIGFADHVGFRSGICYDYPVYDVVQR
                     KPLEIVEKPLVVMEKTVIDGNYMNTVSADRTINLIKELAGVTELFKGSFILLWHNSRL
                     SEKKEIELYSRIIKNIF"
     gene            11436..12836
                     /locus_tag="PL056_01955"
     CDS             11436..12836
                     /locus_tag="PL056_01955"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPM45810.1"
                     /translation="MNGIRLSESLKILISMVVSFLLGISFIADNFVVFLFTALLAILF
                     LITVVALNPALFVCVFFSLSYPVSLTIGKLGINNLGLSSLLIFSMLSMAVLFMRNNFS
                     IDKNRLKNILPFFPIIIVVVLSSFYGFIKSGNDSLEILIKFLVFGVFPALFIMIIPFK
                     KEIFLYIIDMTYYVVLSIFGGFTIAYMFFKIVSPSDFMKFFLAFDNPIGTSLFMIQFI
                     LVSVIYLTINRDIPIIRKVFTYGTIFMALFFVVSAFQRSFLIAIGGGVIYYILTTYRI
                     GFKMVFIIALSTILFGTILYNITLIINPDNMKKVEKTIELAEKLSENKDIMRVKEAEE
                     LGTIGYRIIKIIASKRKIKENPYFGNGLGTFSNYAGYKYPHNIFIEFLYSSGFTGFFI
                     FIIFFGYVLARIQIIFNKAESVMVKAISKYVMSFIVLCILILQFSGGIMNIFPYVFFC
                     SFSMFQLLSIYSGNEK"
     gene            12833..13945
                     /locus_tag="PL056_01960"
     CDS             12833..13945
                     /locus_tag="PL056_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45811.1"
                     /translation="MRIVIFAYNYEPAAGVGVLRPAYWAKHMNEIDPEIICDVITAQE
                     NSTLPVLPGGGRVITVKNEGHFLNRFKLDAGAAWTKYLERFFYNTREKYDVAIFTGGP
                     FVHFFFAEILKRKFNSKIIFDFRDPFANNPNHRLNVFKRYIKRAVERKMLKIPDVSIT
                     VNKVCRDLLEKDEFTRVEIIDNGYDETFLVNPKTLIEKEKHRIVYAGKFYKGASPENL
                     FKVLKQRQESVEFDYMGPHSETVLLVNSRNFLISGTVTYMEAFQRIAEADTGIILTGG
                     EPFESTTKVFDYIGLEKNILIITNGEPKAGILHEITKGYPNVFWARNSENDIRNVLEG
                     VMYHIPKQYPEKEKFSRRAGLIKLVNIINRLFEEKK"
     gene            13947..15074
                     /locus_tag="PL056_01965"
     CDS             13947..15074
                     /locus_tag="PL056_01965"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPM45812.1"
                     /translation="MKIFFLSDGSSIHTKKWVNSLIDNGLTIHLFSLSRFDINDYPLE
                     NFSYTFFDIQRRISVCAGFGKINYLRVLPALKKEIKKFVPDIVHAHFASSYGLLGALS
                     GFHPFVISVWGTDVFEFPEKSFVHRMVLRFNLSKADKILSTSHVMAEKTSCYTNKEIA
                     VTPFGIDLNVFKKEKVSRSELSPFNDKDLVVGTVKLLEKKYGINFLIEAFSIVTRKHP
                     DIPLKLLIVGDGNERKTLEKMVENLGLKEKVHFKGMVPHLKVSQYHKIIDIFIALSEE
                     ESFGVAIVEAGACEKPVVVSDAGGLPEVVDDGVTGFVIKRKNSYAASKALEILVLDEE
                     LRVRMGVAGRKRVEKLYNWKDNVDTMIEIYINILEMKRKSR"
     gene            15109..16320
                     /locus_tag="PL056_01970"
     CDS             15109..16320
                     /locus_tag="PL056_01970"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPM45813.1"
                     /translation="MNIWIINETTGSRIHGMVFRPYYLAKEFVKKGYEVVIFSGSFSH
                     IYSNPPEVEGNTRKETIDGIEYCWIKTPKYSRSQSLGRIFNAFVYVLKMFLINKKEFG
                     KPDVVIVTSPTPFSIINGYYYKKKFNARLIFEVRDIWPLTLIEIGSISKKHPFVVFTQ
                     FFENFSYKVSDRVVSVLPNSKNHMISHGMKEDKFVHIPNGIDIEEICNAKDIDPEVKN
                     RIPEKKFIVMYAGKIGISNALEYLLFAAGKLRNNDQIHIVIAGDGSEKQRFIKLKEEE
                     KLENVTFIDPVKKEQVPSLLSFADVFYIGWHKNNLYKYGISANKLFDYMYAGKPVIHS
                     VQIESNDSVKESASGISVEPENPDAIANAVLKLYNMSENDRMEIGRKGKEHILLNHTY
                     EKLSDSYIKIF"
     gene            complement(16337..16903)
                     /locus_tag="PL056_01975"
     CDS             complement(16337..16903)
                     /locus_tag="PL056_01975"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HPM45814.1"
                     /translation="MKNIVIFGAPGAGKGTQCKMLSEKLGLIHISTGDLIRKEIAEES
                     EVGIKAREIISKGLLLDDTTVSEIFINALKKHLNSPGFLFDGYPRTVKQAEILEEILK
                     DLNMEVNLFIELTLSPDEAVKRLLKRAEIEGRSDDNPETVRARFEEYNRKTAPVRNHY
                     IKKGIYKGIEGVGKIEDINGLIVKIILH"
     gene            17004..17612
                     /locus_tag="PL056_01980"
     CDS             17004..17612
                     /locus_tag="PL056_01980"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HPM45815.1"
                     /translation="MNVMKKGRTLALFDFDGTITNRDTFIDFFKFVYGPWKIAGIAAK
                     IAPFIILHFLKIYPGSKLKELFFGDLIKGWSRERFAQEAGNYYEKRLKKIIKTSALNE
                     IKKLKNEGAQIVVVTASAKEWVIPFTDDLGINIIATELEVVDDLYTGNIKGKNCRGEE
                     KVQRISDKYDTTLFDTVLAYGDSSGDKEMLEFATKGFYRIFH"
     gene            17700..19157
                     /locus_tag="PL056_01985"
     CDS             17700..19157
                     /locus_tag="PL056_01985"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPM45816.1"
                     /translation="MVKSKDEVIESLKNRIYELENQISHIENSVRDEYLHSKEWVKHA
                     LEKLVYFAAIELQPASLDVSNHKIVSFLGDDFFGYSYNGFFTFNQRSGKWEVLAQQGE
                     CTVDISSMPEFKGSMVMEEDREIYHFFDIAKNSYLIHVSLVGKDKKFSEHDFSFISLF
                     VVLATSFYSMKVLDKEVQDKMIENSNMRSAARIVNGLKDNTITLEDAFIELIQNLNID
                     EFVIARKSMENGDLKIVLSDGTDARNWDSFLKTVYEDEDYASDEWLLLPMFDDMLTVY
                     GASVFKLSHNLSIRSVQERVLESVIPQFTETLSNKKLHKDSITDELTGAFNRRYIMKI
                     LDDRFNRCLSNPEAKLSVAMIDIDNFKSVNDTYGHLAGDAVLKKVVEAFNKTLREVDI
                     IGRYGGEEFLIIISTKSHVAAKVCERIREAVESMRIEWNGKDLWVTISIGFVSASPEI
                     LTVEEMVALSDLCLYEAKKTGKNRVVEYKGARGEF"
     gene            19154..>19468
                     /locus_tag="PL056_01990"
     CDS             19154..>19468
                     /locus_tag="PL056_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45817.1"
                     /translation="MKTRMIFTIIVFNLIFFNTSAQDTAGYNNQLLFAVQALNIEKAR
                     QAIRNGANVNAIDDDGWPLFISAVNSDNEGMINLFLRSGVKVDVTGPDGKAVTTYSEG
                     TKT"
BASE COUNT         6233 a         3122 c         4020 g         6093 t
ORIGIN      
        1 aaaggtgttg aatcgatact caatccttac tgcgaatatg cccttgacat ggcggttgca
       61 ctaaagaaaa ggttaaaaga tgttaagatc actgctttta cgatggggcc tgattctgct
      121 gaacttgttt taagaagagc tctttcgctc ggggctgatg aagccgttct gataagtgac
      181 agggcttttt ccggctctga ctgctgggct acggcatata cattgtcaaa ggggattaga
      241 catcagctca aagatccaga tctgataatt tgcggcaaac aggcgataga tggtgatacg
      301 gcacaggtcc cgggtgaaat ttctgaaata cttaatatgc cgttgctgcc gtatgtcact
      361 gaaatagttg atataaatga aatcatcagt gtcaagtgcg aaatgggtga caatatcctt
      421 gaactgtcat caaagcttcc tgtagtaata acagtcggga acggttccaa cataagaagc
      481 atcccttctg ttaaagatgc tgccgctgca ttttcagcga atattgtcca tcttgacaac
      541 accattatca aatgtgatct tactcagacg ggtctgaaag ggagtcccac aagggtagtg
      601 aaaatagagc cggtgacagc aaacggaaaa tgtgagttct acaatgagga aaatcttgac
      661 aaagggttga ataagttgtt cgatttaatc agtattgtaa agggggacaa agtatgagaa
      721 aaattgaatt tattgatctt aaagctcaat atgcagcata taaaaatgaa atagataatg
      781 caatggaaaa agtttgtctg gaatcatcgt tcataatggg tcaacaggtg ggagagcttg
      841 aaaaaaggct ttctgaattt acgggctctc ctcatgcaat atcatgttca agcggtaccg
      901 atgcgcttct tctcgcttta atggcgatag gcgttaaaga tggtgatgag gttattacaa
      961 ccccgttcac tttcattgca actgctgaag tcatatcgct gctcggggcg aaacctgttt
     1021 ttgctgatat tgatgaaact acatacaacc ttgatcctgg caaaatagaa gagaaaataa
     1081 ctgaaagaac aaaagcgata gttccagtgt gtctttacgg acaggttgct gagatggatg
     1141 aaataaatgc tatagcggca aagcacggaa tattcgtaat tgaagacgcc tgccagagtt
     1201 tcggagctga atataaaggg aaaaaaagct gtaatctttc aaaactcagc tgcactagtt
     1261 ttttcccttc aaaaccgctt ggttgttacg gtgatggtgg agcagttttc acaagtgatg
     1321 ctgagcttgc tgaaaagctg aaaagcatca gagttcatgg tcagacccag agatattatc
     1381 ataaatatat cggaataaac ggtcgtcttg acacaattca ggcggcgatc ctcaatgtga
     1441 aaatggacca ccttgaagaa gaattgaaaa aacgggttga agtggcagat cattattcaa
     1501 agaatcttgc aggaaaccct ggcattgtac tgcctgaaat aaaaagcgac agaacatcgg
     1561 tctatgcaca gtattcaata agagttaaag acagagacgg ctttacggca aagcttaatt
     1621 ctgcaggaat tccgacagca gttcactatc caagaccgct tcatcttcag gaagcttact
     1681 catatctcgg ccttaaaaac ggggatttcc cggtatctga aaaagtcagc aatgagatcc
     1741 tgagcattcc gatgtccccg ttcctgaaaa aagacgatca ggactatatt attgaaaaca
     1801 ttataaaaaa tgtataagga ttgaatcatg aagatcgggc ttgtgtcgaa catgtatcct
     1861 tctgaaaatg ataagaacta cggagttttt gttaaagggc ttgtcgattc aatgtcgaaa
     1921 aaaggggttg agttcagctt cacagcagtt ataaaaggaa aacaaaacgg aagatggcaa
     1981 aaaatatggg catatcttaa actttatttc tcagcggttt tcaaccttct ttttaaagag
     2041 tgtgatatcg tttttcttca ttatcctctt catcttgcac cacttatcta tatgatgaac
     2101 atctttatga tcaaaccgct cgttgtcaat tttcacggca ctgacatgaa tcctcagtca
     2161 aaaggacttc aagtgcttga aaatattctt aaaagatctc ttgacaatgt taaaatggtt
     2221 gtagtccctt caagttttct gaaaaaagaa gtcatgaaaa aatatggagt acctgaagaa
     2281 aagatatttg tttctccttc cggaggagtt gattttgaca agttcagatc atttgatgac
     2341 aatcctgcag ctgtaaaacc ttatctggct ggttatgtct cgagattgac taaagaaaaa
     2401 gggtgtgatg tttttcttga atctgtaatc tcgctgaaaa gagaaggttc ggttgaagga
     2461 aaaaaatttc ttattgcggg cactggtccg atgcagaatg aaatatctga tacgataaga
     2521 aaagaaggac ttcaagaaac agttgaattt aaaaatataa acgaccagtc gatgctgccg
     2581 caatttttca atgatatgga actgtttgta ttcccgtcac tgagtgagag tctcgggctt
     2641 gtaggacttg aggcgatggc ctgcggaacc cccgtgacag gatcagagat cggaggaata
     2701 tcagattatc ttactgacgg acaaaatgga tatttcttta aagcaggaga tccttctgat
     2761 cttgcacaga agattagaca atttatgctg ttaaccagaa tcgaaaaaat gagattcaag
     2821 gaaaatgcaa aaaacaccgc aactcaatat gagagggaaa gggtggtttc catgcttatt
     2881 aggatgttgg aattattgtt gttttaaatg aatcaagaat cttcaggaat cacaggtctt
     2941 ttgataattt gcatttaaag tgatattaaa atattatgtt gtcgaaattt atgcactttt
     3001 ttgaggattt agcatatgga aaaagagctt ggcaaagaga aaaatgaaat atcattaaaa
     3061 gatgtctacc ttgtcctggt aaataacaaa aaactttatc ttattttctc tattttgaca
     3121 ttttgttctt tggctgtgat cttccttttt ttcctgacgc ctgtttatga agtcaatgct
     3181 tcattggaaa ttcgtcagca gaatgcatct ccactatcaa taaatccgat ggctgcaatg
     3241 ctcataggcg gaacagcaat gagtgataag acaattgatt atgaggtttt gagaagcaga
     3301 agaattctca attcgataat tgagaacaaa ggtcttcaga tgaatattac aaaaaaatgg
     3361 aatactatgt tcagttattt cctggggctt ttgtttaaaa acctgccaag cgattcattc
     3421 gtttatttta aaaagatacc tgatgttttg aaaagtgaat caggaaagat tgatgctaaa
     3481 gaagaagggt actcaatata tttcaatgat gaaaaagccg aatgtttatg ggacaaagaa
     3541 tgttctttta tgggatctgc agttcagctt gataaaatcg gaacattttc agccgaaatg
     3601 acttttaaat ttgaatatga acaaattgtc gatgcccgtg atagagtgaa tggctggatt
     3661 aatatcggga aagctgaaga atctgaaatg atacttcttg gacttgttca cgagagtccg
     3721 acaatgggga gtctaatatt gaacgatttg gtaaatgctt atgtaaatat taaatctgac
     3781 tgggaaaaag aagacatcac agcaaaaaag aaatatataa attcaactct tgatgaattg
     3841 tccgtaagtc ttgatgaaaa aagtcagaag atgattcttt ttcagcagac agaaaaaact
     3901 ataatgccgg aagttgaaat ccccgagctt cttaaaaagc aggaaacatt aaaaatacag
     3961 atagaagaat taaaatttaa gaaaaagatc atagccggtg ctatggaaaa tattggaaag
     4021 tctctggaca caccaatttc cgtgccattt gcttttgaag atctatctac tcaggaggca
     4081 ttgaaatctt ataacgatct tctgttcagg aagagcaggt tgtctcaaag agttacagaa
     4141 gatcatccga tgttgtcagc gatagaaaaa gaaattgaga cgaacactga ttctttgaaa
     4201 aagatgctta aagaaacggc agatcagtat gataaaggca ttaatattct taatgaactg
     4261 ctatcaatga tttcaagtgg acaacacaaa atccccgaga atcttttctc atacttaaga
     4321 cttaaaaggg atgtggaact tgcggaaaag gtatttgtca ctctttcagc taaactttat
     4381 gaatctgcaa tagagccgaa tgtaggcatc gcccctgtaa ggttgataga tctgccggat
     4441 ccgtttgtaa aaaggtattt ccccaaggca aagatttttg cggtaatcat atttattatt
     4501 acattattct ccggtgcggt tgctatcttt attaaagaat ttcttaaagc agttttgaaa
     4561 agcattaagt gagtggtttt ttatgaaaat cagcaaaacc atcattggcg gtgcgatagg
     4621 aaactgtgtt catgtcgccg gcgtttttag ttatatccag atcgcagaac agaacggata
     4681 caaaggtgtt tttctggggt ctgcagttga tcctgatctt tttgcttcaa aaattaaaga
     4741 gttgtctccg gatattgttt gcataagtta cagattgagt gcttcagcaa tttatccgat
     4801 attaaagagg ttttttgatt ctctcggttc tttggaaaag tgtgccggca tgcttttcta
     4861 ttttggggga acacctgaaa caatagagat agcaaaaaaa tttccacgat tcagcaggtt
     4921 ttttcaaggc gaagaaccac ttgaaattat ttacagctct ttgaatatta aaaatgatat
     4981 agagattatt gcattgcgtg gtccggaatc atcagcaaaa gttcctgtta tgaatccttc
     5041 tttaaaaaac agactttcgc agaagacata ttttccgatg atccggcatc acttcggtct
     5101 tcccacaatg aaagagaccc tcgaaggcat aagacagatt gccgattcag gtgtagttga
     5161 tgttatatcg atcgcaaccg accagaatgc acaggagttt ttcttttcac ctgaacaaat
     5221 gaaccctttg ctggatggag ccggaggagt tcctgtaaga acagagagtg atctttttca
     5281 gatttatgat gctgccggca gagggaactt tccgttgtta aggatatatt ccggaaccag
     5341 agacctttta agatgggctg aaatgagtct cagaacaata aacaatgcat ggggagcaat
     5401 cccgcttttc tggtatagtc aacttgacgg cagatcaaaa agaagtgttg cagaagccat
     5461 atctgaacac caggaagcaa taaaatggta tgcagatcat ggaattccgg tcgaaataaa
     5521 tgattcccac cattggagtt taagagatgc tcctgatgtt atggctgtgg ttgatgctta
     5581 tatttcggca tacaacgcaa aaaagttggg tgtgaaaatt tatattgccc agtttatgtt
     5641 taacacgccg aggattacaa gcggcagaat ggatcttgca aaaatgttgg cgaaagacac
     5701 gcttatatca gaattagtgg atgatgattt tcaatacatt aaacaggtta gagccggatt
     5761 gacacatttt tcaattgatc agtcagttgc taagggtcag ttggctgcat ctacagttct
     5821 gtctatggcg attaaaccgc atatacttca tgttgtgagt ttttctgaag cagatcatgc
     5881 agctaaaccg gaagatgtaa ttgaaagcgt taagatagca aaaggtgtgt tgaaaaactg
     5941 catatatgag tttcctgatc tctcaaatga tccaatagtg caggaaagaa aaaacaacct
     6001 gatccaagag gccaggaaag ttttagctgt catgattgaa acaggatctt gttatgatga
     6061 tccgttaata tcaagtgaat atcttgctac tgctgttttg tcaggttttc ttgatgcgcc
     6121 tcacttaaag ggcaatccta atgcaaaagg ttctatcagg acaatgcctg taaacggagg
     6181 atatgaaatt gtcgatgaaa ataataaagt tattagtgct tccgaatatg tttcgcggat
     6241 tatagaattt agaaaaaagg gaggatcgat tggttaatcc ggcacctgta accataatag
     6301 gagcaggaaa ttcaggactt gcgatggcgg cacatctttc tttaaacggt catccggtct
     6361 gtttgtggaa tagaagcttg aaaacacttg aaaatattat ttcggaaggg caggggagca
     6421 ttcttgcgga aggagtggtt aatggaaatg cgaagataga tcttataact ggcgacattg
     6481 aaaaagccgt taatttctca aggataataa tggttacaac tccggctgat gctcacagta
     6541 atatttccat gctctcagca ccatatctga aaaaagacca tattgtaatt ctaaatcccg
     6601 ggagatgttt cggagcgatt gattttttaa aaaaactttc agataaaaat tgtaaagatt
     6661 tgccaacagt tgctgaaact caaacaatat tgtatacatg tagaaaaatg ccacctaacc
     6721 ggtctctgat tctggcatta aagaaaaaag ttcttatttc ttcaacaaac tttaaaaata
     6781 caatgaaagt aatggaaaat ctccctgatt gtattagacc atatttccat ccggccggaa
     6841 gtttgcttga aacatctttg ggaaatgttg gaatgatcct tcactgtctt ccggtactta
     6901 tgaatgtagg gtggatagaa aacaagaaaa caagctttaa atattattat gacgggataa
     6961 ctccctcaat tgccaagctt cttgaaaaaa ttgataatga aagactggat gttgccagac
     7021 atcttggttt gactgtcccg tcattatctg cctggcttga ggtcacttac ggggtgtcag
     7081 gggagagtct ttacgataaa attcaaggag ttgaatcata tagaacaata gaggcacttg
     7141 atacgcttga acaccgttat cttcttgaag atattccgtt tggtcttgtt ccgatagagg
     7201 acataggcaa acagattgga ttaacaatga agataacaac cttaataatt gaacttgcaa
     7261 atgaaatttt cggttttgat ttcagagata aaggaagaac cctgaaatcg cttggaatat
     7321 cactttctga tttgatatta tggaataaaa atgaataaat ataaaaacca tgcggttatt
     7381 tgcggttctt atgttaatgc tctggggctt gcaagaagtc tcggagcaga aggaattgct
     7441 gtaactgtaa tagattatac aaaaaacata tcgttctatt caaaatatgt tcatggaatc
     7501 gtatgtccca aaccggatga ggatgaggaa gggttcattt cattcctgat cgatctgggg
     7561 cacagatttg atgaaaaacc tgtgatcttt gttacaaatg acatatggct tattcctgta
     7621 agccgcaatg gagagagatt aagcaaatat taccacatac ctatgtcaga ctgggaaatt
     7681 atcaaacagt gttccgataa gacatatatg tataagatcg ctgaagaaaa caacattcct
     7741 tatcccaaaa cactgttcct tgaaaaaagc tcacaatttt taaagaaaga gcatagcttg
     7801 aaatatccgg ttatcctgaa accgtctgtt acaataggtt tcagtgaaaa gctgaagtct
     7861 tacggcagaa gccttgaatt taaagatcag tcttctgctg agaactggat tgataaaatc
     7921 agaaatgctc aactcgatga tgttccggtt gttgttcagg agatgatccc cggcggagca
     7981 gaaaaccttt ttacttttac atcatattca agaaagggcg gtgagacagt cgcatattca
     8041 accggacaca agataagaca gtctcctcct cacgctggaa cgataatatc agggcatgtc
     8101 atccccaata aagatgtgat tgaaaacggc atcaatgtta ttaaaaaaat gaaattcgac
     8161 ggtattgcca atacagaatt taaatatgat catcgagacg gattgtacaa attgattgag
     8221 ataaatccaa gacccggcat gtggaatttc tcagtaatgg catcagggat aaatcttcct
     8281 ttatatgcct atctcgattc gctgggaatt gactataaac ctgttaaatc cagtgataaa
     8341 gaattgaact gggttttctg gtcgagtgat ctttattatt ctttatttgg atttaaaaaa
     8401 atgggttttc ctgaatattc cttgactctt tgcagttggt ggaagtctct taaaggtaaa
     8461 aaagtaagtg cggttttctc tctcatggac attaagccag cactgtttct gttcagttct
     8521 tttttttaca gtaagttaaa acggtttttt taatgtcatc taaactcgct cagtcagcta
     8581 tttcaacctc ttttatctgg aatttcgggg ctgtagtttt cagaagaatt ataaatgtga
     8641 tcgcgatacc tgtgatagcc cgactcgttt caaaagaaga ttatggaaat atccaaataa
     8701 ttataagcac ttttatgctt cttgcggtta taaggaattc agggtttaat gactacttta
     8761 ttaaagaagc agacgatagg gacaaaaagt tcttttcatt gtttgtaaat acaatgtatc
     8821 tgaatacttt tgttattgct gtgatattta ttccagctgt gggaatttac agttatttca
     8881 atgatgtgtc ttcaatttat acctatcttg ttttaatagt tgttcttctt tttatcaatg
     8941 tcggtgagat tgctggagaa acttatttca gacagaaagt gtgttttaag aatatcgcta
     9001 ttgcaggcag cataaaaatt ctttcctatc cgattttcac gataatattt cttctgataa
     9061 gacccgatct tttttcactg tttgccgcat ttgccctttc tatagtagct gagattataa
     9121 gttacttttt ttttcttcct gacaacaagg tttttctgcc tgatataaag aaaatgacag
     9181 aagggtttct actgcagttt aaagaaaggg ttttctgttt cagtgtcagt gcaggaatgt
     9241 tcatttcaaa ttttaacgta tcatttccga tctatctctt tgcatatgca ttttcggcag
     9301 aacattcggc acaatatgca atttcagccc aattggtatc aattccgttc agtttgataa
     9361 ttgattcact ttcaaaggtt atatatccgg tatttatcaa gcaaaatgaa gaagagagaa
     9421 ggcgtttcta tctgtcaata ttgaaattct ttccgttcat tctctatccg atgctcctat
     9481 ggataatgat atatgctgaa ggagttgttt ctctttttct gggagaaaaa tggattgatg
     9541 ttgtgccgat aataagaatt gtgtctatac cggtattttt caatgttctc tcaagtccat
     9601 taggaccagt tgccactgcg ttgggaaagc cgcattacag agttttgtgg tcaggtggca
     9661 gtgtgttgct tgctctttta gcaggaagta tgggattgca attcggtttt ttatggggag
     9721 ttgcatctgt aatttcatca agagtggtaa ccctcattgt atggcaggtt gttgcatgca
     9781 gaatgattaa cctttctcat tttaaacttg ctgtacagtt gataccatat gttccttttt
     9841 ggataatgct tgtcgttttc tggatattct ttccggaagt ttccagcttc ccttatgtca
     9901 gaatgtttgt ttccggaaca gttggaatgt tgatatactt tttgctgttt atgcttttct
     9961 ttaaaaatga tgttatgcag ataaagaaac ttgtaaatga tctttttaaa aaaaaggttt
    10021 ttgaatgaac tttaaagtga ccgttcctga taattttttt gcggaaagac agtatatagc
    10081 gaatgtcatg ttgaaggaat tccttggtct ggattatgac atcgaacaag ttgctgatgc
    10141 aaagacagta aaaatctcaa ataatgaaaa cacccgagag atcacttttc ctgatgtatt
    10201 cttttcgatg gatgaatctg actggctaaa agaaagatct cttcccaaag agccgcttaa
    10261 aattattaac agctctgttt ttccaggcaa cccgatactc tcatcggatc gaatcccgct
    10321 tatttacggc acaaatgaaa gtgaagttag tgtctctgaa gaagaaattt ccattcccgt
    10381 agatgttttc gggagtgcgt tttttatgtt gacgcgttat gaagaggcgg ttaaaaataa
    10441 ccgcgacact catgaccgct tcccggcgag tgcgtctttg gcttttaaag aacactttct
    10501 ttgccgtccg ataattaatg aatatgttga gattttatgg tcttcaatga gagttttgtg
    10561 gcctgagatc gaaagaaaag aaagaaaatt tgaaatggtt ttaagtcatg atgttgacag
    10621 accatttaaa tatcggaata tgcttccagc aacatttttg aaaattgttg cagcagattt
    10681 aattagaaga cggtctgtaa gaaaagcata cgaaagcgtt aaaagttata tagcggttaa
    10741 aatgggtgaa atagacagag atccctttaa cacatttgat tttattatgg atctgagtga
    10801 aaaggctgga ttgaaaagct gtttttattt tatttctgat cattcagata acaagatcaa
    10861 ttgcgattat tcaataaatg atcctgaaat gatcagtctg atgaaaaaga tacacagcag
    10921 aggacatgag ataggtctgc atcccggata cgatacttct gataaccctg aaaagcttaa
    10981 aaatgaattt gtaaaactcc ggaagatatg ttctgcaaac ggcattaaac aggaaaaatg
    11041 ggggtccagg cagcattatc ttcgattcag tgtgcctcaa acatggcgct atctctctga
    11101 agctggaatt gattatgact cttctatcgg atttgccgat catgtcggtt ttagaagcgg
    11161 gatatgctat gattatccgg tttatgatgt tgttcaaaga aaaccgcttg aaatcgttga
    11221 aaaaccgctt gtggttatgg aaaaaacggt tattgatgga aattacatga acactgtttc
    11281 tgcagaccgc actataaatt tgatcaaaga attagcaggt gttactgaat tatttaaagg
    11341 aagttttatc cttttgtggc ataacagcag attgtcggaa aaaaaagaaa ttgaacttta
    11401 ttcaagaata ataaaaaaca ttttttgagc attgtatgaa cggaataaga ttgtcagaga
    11461 gtttaaaaat acttatttca atggtcgttt cttttctgct cggcatttca ttcattgcgg
    11521 acaactttgt tgttttcctc tttaccgcat tattagccat cttgtttctt attacggtcg
    11581 ttgctttgaa tcctgctctg ttcgtttgtg ttttcttttc tctttcatat cctgtttctc
    11641 ttacaatagg taagcttgga ataaacaacc ttggtctttc ttctttatta attttttcga
    11701 tgctttcaat ggcagtgctt tttatgagaa acaatttttc catagataaa aacagattaa
    11761 aaaacattct tccttttttt ccgattatta ttgtcgttgt gctaagttct ttttacggtt
    11821 tcatcaaatc cggcaatgat tcattagaaa ttcttattaa atttcttgtt ttcggtgttt
    11881 ttccggcatt atttataatg ataattccgt ttaaaaagga gatattctta tatataatag
    11941 acatgacata ctatgtagtg ttgtcgattt tcggaggatt tactattgct tatatgtttt
    12001 ttaagattgt aagtccgtct gattttatga agttttttct ggcatttgat aatcctatag
    12061 ggacttcgct ttttatgatt cagttcattc tggtttcagt gatctatttg acaataaaca
    12121 gagacattcc gataattaga aaagttttta catacggcac aatctttatg gcattgtttt
    12181 ttgtagtatc ggcttttcag agaagttttc tcatagctat tggcggtgga gtgatatatt
    12241 atatattaac cacatataga ataggattca aaatggtttt catcatagct ctttctacaa
    12301 tattattcgg gacaattctt tacaacataa cactgataat caatcctgac aacatgaaaa
    12361 aagttgaaaa aacaatagaa ttggctgaaa aactcagtga gaataaagat attatgcgtg
    12421 ttaaagaagc tgaagaactt ggcacgattg ggtacaggat aataaaaata attgcgtcaa
    12481 aaaggaaaat taaagaaaac ccctatttcg gaaacggact tggaacattt agcaattatg
    12541 ccggttataa atatccccat aatattttta tagaatttct ttattcttcg ggttttacag
    12601 gtttttttat atttattatc tttttcggat atgttcttgc caggatacag ataatcttta
    12661 ataaagcaga aagtgttatg gttaaagcga tatcgaagta tgtgatgtca tttatagttt
    12721 tatgcatttt aattttgcag ttctcaggag gaattatgaa catatttcca tatgtttttt
    12781 tctgttcgtt ttcaatgttt cagttgttaa gcatttattc tggaaatgaa aaatgagaat
    12841 agttatcttt gcatataact atgaacctgc tgcaggagtt ggtgtattaa gacctgcata
    12901 ctgggcaaaa cacatgaacg agatagatcc tgagataatc tgcgatgtta tcactgccca
    12961 ggaaaacagt acactgcctg ttctgcctgg cggaggacga gttataactg taaaaaatga
    13021 gggacacttt cttaatagat ttaaacttga tgccggagca gcttggacaa aatatcttga
    13081 gagatttttt tataacacaa gagaaaaata tgatgttgca atatttactg gagggccgtt
    13141 tgttcatttc ttttttgcag aaatattaaa aaggaaattt aattcaaaaa taatctttga
    13201 tttcagagat ccgtttgcta acaacccgaa tcacagactt aatgtattca aaagatatat
    13261 taaaagggct gttgagagaa agatgcttaa aattcctgat gtttccataa ctgtgaataa
    13321 ggtttgtcga gatcttctgg aaaaagatga gtttacaaga gttgaaataa ttgataacgg
    13381 atacgatgaa acttttcttg tcaatcctaa aacactaatt gaaaaagaaa agcacagaat
    13441 cgtttatgcc ggcaagttct ataaaggagc ttctcctgaa aatttattta aagttttaaa
    13501 gcaaagacag gaaagcgttg agtttgacta tatggggcct catagtgaaa ccgttttatt
    13561 ggttaacagc agaaactttc taatttcagg gacagtaaca tatatggaag catttcaaag
    13621 aattgctgaa gcagatactg gtattatctt aacgggagga gagccatttg aatcgactac
    13681 aaaggttttt gattatatcg gtcttgaaaa aaacatactc ataattacaa acggagaacc
    13741 caaagcaggt atcttacacg aaataacaaa gggttatcct aatgttttct gggcacgcaa
    13801 tagcgaaaat gatatccgca atgtgttaga aggagtgatg tatcacatac cgaaacagta
    13861 tcccgaaaaa gaaaagtttt caagaagagc cggtctgatt aaacttgtta atattattaa
    13921 ccgtttattt gaggaaaaga aatagtatga aaatattttt cctttcagac ggatcgtcaa
    13981 ttcatacaaa aaaatgggtt aattctttaa tagacaacgg attgacaata catttattca
    14041 gcttaagcag gtttgatatt aatgactatc ctttggaaaa tttctcttat actttttttg
    14101 acatacaaag gagaatatca gtttgtgccg gatttggaaa aataaattac cttcgagttc
    14161 ttccagcctt gaaaaaggaa ataaaaaaat ttgtacccga tattgttcat gctcatttcg
    14221 cttcgagtta cggacttctt ggtgctttaa gcggtttcca tccgtttgtt atttcggtgt
    14281 gggggactga tgtttttgaa tttcctgaaa aatcgtttgt tcatcgaatg gttttgaggt
    14341 ttaatttgtc aaaagctgat aaaattcttt caacaagtca tgttatggct gaaaaaacat
    14401 cttgttatac caacaaagag attgctgtaa caccattcgg aattgatttg aatgtgttta
    14461 agaaagaaaa ggtttcgaga tcagagcttt ctccatttaa tgacaaagat cttgttgtag
    14521 gcactgtgaa actgcttgaa aaaaaatatg gcatcaactt tctgatcgaa gctttttcta
    14581 ttgtaacaag aaaacatccg gatattccgt taaaacttct gatagtaggt gacggaaacg
    14641 aaagaaaaac gcttgagaaa atggttgaaa atctgggatt aaaagaaaaa gttcatttta
    14701 aaggaatggt tcctcatctt aaggtttcgc aatatcataa aattattgat atttttatag
    14761 cattatctga agaagagagt tttggtgttg caattgttga agccggagca tgtgaaaagc
    14821 cagttgttgt ttctgatgcg ggtgggcttc ctgaagtggt tgatgatggc gtcacgggat
    14881 ttgtcattaa gagaaaaaat tcttatgcag catcgaaagc acttgaaata cttgtacttg
    14941 acgaagaatt gcgtgttaga atgggcgtag cgggaagaaa aagagttgaa aagttatata
    15001 actggaaaga caatgttgat acgatgattg aaatttatat aaatatactt gaaatgaaaa
    15061 gaaaaagcag atagcttctg ttgacctggt tattttggtg agaaagaaat gaatatttgg
    15121 ataattaatg aaactactgg atcaagaatc catgggatgg tatttaggcc gtattatctt
    15181 gcaaaagaat ttgtaaaaaa gggttatgaa gttgtcatat tctcaggttc tttctcccat
    15241 atatattcaa atccgccgga ggttgagggg aatacaagaa aagagacaat agacgggatt
    15301 gaatattgct ggattaaaac accgaagtat tcaagatcgc agagtttggg acgaattttc
    15361 aatgcttttg tttatgttct taagatgttt ttaattaata aaaaagagtt tggaaaacct
    15421 gatgttgtaa ttgtgacatc cccaacccct ttttctataa ttaacggtta ttattacaaa
    15481 aagaaattca atgccagact gatttttgaa gttagagata tctggccttt gacattgata
    15541 gaaataggta gtatttcaaa aaaacatcct tttgtagttt tcacacagtt ttttgagaat
    15601 ttctcatata aagtatctga tcgtgtagtt tcagttcttc cgaattcaaa gaaccacatg
    15661 atatcacatg gaatgaaaga ggataagttt gttcatatcc ctaacggtat tgatatagag
    15721 gagatttgca acgcaaaaga tatcgatcca gaagtgaaaa atcgtattcc tgaaaaaaag
    15781 tttattgtaa tgtatgctgg gaagatcgga atttcgaatg cattggaata tctcttgttc
    15841 gcagccggaa aactaaggaa taatgatcaa attcatattg ttatcgctgg tgacggctca
    15901 gaaaaacaac ggtttataaa attaaaagag gaagaaaaac ttgaaaatgt cacttttatt
    15961 gatccggtaa aaaaagaaca ggtgccgtcg ctgctttcat ttgcagatgt tttttatatt
    16021 ggatggcaca aaaacaatct gtataaatat ggaatttcag ctaataaact ctttgattat
    16081 atgtatgcag gcaagcctgt aattcattca gtgcagattg aatcaaacga ttctgttaaa
    16141 gaatcggcat ccggtatttc tgttgagcct gagaatcctg atgctatagc aaatgcggtt
    16201 ttgaaattat acaacatgtc tgaaaatgac cgtatggaaa ttgggagaaa aggcaaagaa
    16261 cacattcttc ttaatcacac atacgaaaaa ctatctgatt cgtatataaa aatattctga
    16321 attttcaggt tatgttttaa tgtaaaatta tcttcactat caaaccattt atatcctcta
    16381 tctttccgac accttcgatg cctttgtata tccctttttt gatgtagtgg tttcttacgg
    16441 gtgcagtttt cctgttgtat tcttcaaaac gggctcttac tgtttccgga ttgtcatcgc
    16501 ttcttccttc tatttcagcc cttttcagaa gccgttttac tgcttcatcc ggtgagagcg
    16561 tgagctcaat gaaaaggttc acttccatat tcaggtcttt aaggatctct tcaagtattt
    16621 cggcctgttt taccgttctt ggatagccgt caaaaagaaa gccgggggag ttgaggtgtt
    16681 ttttcagggc attaatgaaa atttcagaga cggtggtgtc gtcaagtaga agacctttgc
    16741 ttataatttc gcgggctttt attcccactt cactttcttc agcaatctct ttcctgatga
    16801 ggtcgcctgt tgaaatatgt atcaatccga gtttttcaga aagcatttta cattgtgttc
    16861 ctttgcctgc tcctggtgca ccgaagataa cgatgttttt catttttcct cctgttaaaa
    16921 aaagatcggg gtcattataa cattttagaa agggtttgtt aaaatttttt ttactgtttg
    16981 atttttaaac catcagtgat aatatgaatg ttatgaaaaa ggggagaact ctggcacttt
    17041 ttgattttga cggcacaata acaaacaggg atacatttat cgattttttt aagtttgttt
    17101 atggtccatg gaaaattgca ggcatcgctg caaagatcgc cccgtttata attctccact
    17161 ttctgaaaat atatcccgga tcaaagctta aagagctttt ttttggagat ctcataaaag
    17221 ggtggagcag agaaagattt gcgcaggaag ccggtaacta ctatgagaaa agattgaaaa
    17281 agatcataaa aacatcagca ttgaacgaaa ttaaaaaact taaaaacgaa ggggcgcaga
    17341 tagttgttgt gacagcttcg gctaaagagt gggtgatccc ttttactgat gatcttggaa
    17401 taaatattat tgcaacggaa cttgaagttg tcgatgattt atacacggga aatatcaaag
    17461 gtaaaaactg tcgcggagaa gaaaaggtgc aaaggatctc agataaatat gacacaacgc
    17521 tttttgacac agttctcgca tatggtgact catccggcga taaagagatg ctggaattcg
    17581 caaccaaagg attttacagg atttttcact aacttacatg gttgacccat taaatttgaa
    17641 aactaattct ctttctgata tattcatccg tatgtttttt tcacggtgct ggaaacataa
    17701 tggtaaaaag taaggatgag gtcatagaat ctttaaaaaa caggatttat gaacttgaaa
    17761 atcagatttc acacatagaa aattcggtaa gagacgaata tcttcattca aaagaatggg
    17821 taaagcatgc tctggaaaaa ctggtctatt ttgccgcgat cgaacttcaa ccggcttctc
    17881 tcgatgtttc caatcataaa atagtcagtt ttcttggaga tgattttttc ggatacagtt
    17941 acaacggatt ttttactttt aaccaaagat cggggaagtg ggaagtgctt gctcagcaag
    18001 gggaatgtac tgttgatata tcatccatgc ctgaattcaa aggaagcatg gttatggagg
    18061 aagacaggga gatatatcac ttttttgata tagccaaaaa cagttatctg atacatgtat
    18121 ctcttgtcgg taaagataaa aaattttcgg aacatgactt ttcatttatt tcgctttttg
    18181 tcgttcttgc gacatcattt tacagcatga aagttctgga caaggaagtt caggacaaga
    18241 tgatagaaaa ctcaaatatg agaagtgcgg caaggatagt aaacgggctt aaggataaca
    18301 ctataactct ggaagatgca ttcatcgagc tgatccagaa tcttaatatt gatgagtttg
    18361 taatagcaag gaagtcgatg gagaatggtg atttaaaaat agttctttcg gatggcactg
    18421 atgcaaggaa ctgggactct tttctgaaaa cggtatatga agatgaggat tatgcaagtg
    18481 acgagtggct tctgctgccg atgtttgatg atatgctgac tgtttacgga gcatctgttt
    18541 tcaagctttc acataattta agcataagaa gcgtccagga aagagttctt gagtccgtaa
    18601 tccctcagtt tacggaaaca ctttcaaaca aaaaactgca caaggactca ataaccgatg
    18661 aactcacagg tgcttttaac agaagataca taatgaaaat actggatgac agatttaaca
    18721 gatgcctgtc aaaccctgaa gcaaagcttt ctgttgcaat gatagacatt gataatttta
    18781 aaagcgttaa tgatacatac ggtcatctgg cgggggacgc tgtccttaaa aaagtggttg
    18841 aagcttttaa caaaacttta agggaagtcg atataatcgg aagatacggc ggagaggagt
    18901 tcctcatcat aatttctaca aagagtcatg ttgccgcaaa ggtctgtgaa aggatcaggg
    18961 aagctgtgga gtcaatgcgg atagaatgga acggaaaaga tctatgggtg acaatatcaa
    19021 taggatttgt ttcggccagt cctgagatac tgactgttga agagatggtt gcgttatctg
    19081 acctttgtct atacgaggca aaaaaaacgg gaaagaacag agttgttgaa tataaaggag
    19141 cgagaggtga attttgaaga ctaggatgat ttttaccatt attgtattta acctcatttt
    19201 tttcaacaca agtgcacagg atactgccgg atacaacaac cagctccttt tcgctgttca
    19261 ggcactgaat attgaaaagg caaggcaggc aatccggaat ggtgcgaatg tgaatgcaat
    19321 agacgatgac ggctggcctt tgttcatttc tgctgttaac agcgataacg aggggatgat
    19381 caatctgttt ttaagaagcg gtgtaaaagt agatgtgacc ggacctgacg gcaaagctgt
    19441 aaccacctac tctgagggca cgaagacc
//
LOCUS       DAOOYB010000018        19380 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_2704_length_19380_cov_10.287038, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000018 DAOOYB010000000
VERSION     DAOOYB010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 19380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19380
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2704_length_19380_cov_10.287038"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..454)
                     /locus_tag="PL056_01995"
     CDS             complement(98..454)
                     /locus_tag="PL056_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45818.1"
                     /translation="MKKLLIVAVFGIILFVSCGSETTLADAQEDAARVYCEKLFTCDE
                     GIAARPMFGGTEAKCREAMLALMEQEANDDPCTNFNADKADECSTCSENLSCSETFPA
                     EEDVEDPCPVCDQVCD"
     gene            complement(522..1076)
                     /gene="nadD"
                     /locus_tag="PL056_02000"
     CDS             complement(522..1076)
                     /gene="nadD"
                     /locus_tag="PL056_02000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HPM45819.1"
                     /translation="MSNNIKDIAVFGGKFDPPHLAHRMTIDLALEKYKMDEVWLIPSF
                     NHPFGFKTTSFEHRLEMCRILAKQWPEKRIKVLETEREINSGFTIDVLKHFSKLYPEY
                     NFHLFIGADNWKNREKWKEFNELERICKSIKVIGRGKDKFDGSALPDISSTMIKQMIH
                     DKERVALLLPEGIEYYIRKNSLYI"
     gene            complement(1069..1857)
                     /locus_tag="PL056_02005"
     CDS             complement(1069..1857)
                     /locus_tag="PL056_02005"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005883671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HPM45820.1"
                     /translation="MIQLQEINYSKAVNFIVNSIQNEMNRNGHSKAVLGLSGGIDSAF
                     VATLASLALGKENVTGILMPYKSSSRASVEDAMKMVHHLGINHEHIEITSAVDAIAAN
                     NMVNPEISRHIRLGNIMARVRMITLFDYSSAKKAIVFGTGNKSEIELGYFTLFGDSAS
                     AINPVGYLYKTDVFKISKYLGIPESLITKAPSADLFEGQTDESEIGFSYADMDPVIYA
                     LTDLKMNPDEVIKAGHDEKLVMALNKRINAMSFKRNIPVILKYE"
     gene            complement(1858..2343)
                     /locus_tag="PL056_02010"
     CDS             complement(1858..2343)
                     /locus_tag="PL056_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45821.1"
                     /translation="MDTFKGTIVAQTKADRGFWYTIFSNEGTHRFFSRKEIFSLFENC
                     EVVLEKKNNCFFVSDYSTLDETPPLKSRPDNIIAAFWLSRLADSLTFSDSAEFDFIEK
                     CRNAVATDFNKVALDTIENHYIQVSGYNSSDEPEKIIHDCFFSSIKLRRSLINQISLR
                     K"
     gene            complement(2343..3173)
                     /locus_tag="PL056_02015"
     CDS             complement(2343..3173)
                     /locus_tag="PL056_02015"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPM45822.1"
                     /translation="MKRVLSVFLVAAALFSFSCKSAEPKTVSDEKKADSYYQMGLAAW
                     NQGDYIKATREFSKAIEAAPEIPQYYNHLGMVYLQDGQYDKAEENLKKAMTIDPKYSD
                     ARNNLGVLYTRLGKYDQALEELKNVSSDALYPFPHYVETNIGIVHRLQKQYDIAEKHF
                     NSAIKMKGTYCEPYKELGVMYDEQALYEKAADNYKKAISFCPYYVEALYRGALKMYML
                     KQEKTGEIYLLRCLDVDAKNIKEVTIPFLKDCLDLAAKVGVTSDMQKSKKNKQQIDSE
                     "
     gene            complement(3177..3689)
                     /locus_tag="PL056_02020"
     CDS             complement(3177..3689)
                     /locus_tag="PL056_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45823.1"
                     /translation="MKKGLFLLIVLGFFQIFAGDLFINGVKATGMKDTELKNCNVKID
                     SSGNIHIDAPGVKIVDESAKPSKEYIISISFDKPLTNDFVFFINGKEAVKVMKGQQDS
                     ILEINPFLKKGENVVAYNSVPNPEVIKFTIVAGTGIKKGSSIEFAPFAEHKGEINNLG
                     ASGNFKIVAE"
     gene            complement(3693..5720)
                     /locus_tag="PL056_02025"
     CDS             complement(3693..5720)
                     /locus_tag="PL056_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45824.1"
                     /translation="MQKIKFFVITLCAAIFFFPLFAQDDETKEETVKPAGEEKTEKEE
                     KKEEPTEEKKEPPAFYPQDELVEPPAPAQQPQTVTPQSEPAPEPEPPAQPSTPQPDTA
                     PAPQPEPAPEIPAQPEPVTQPEPVIAPAPETPAKPADEQKKDETPKDEKPKEEKVFWK
                     GELANIGSKEILSPYNSAGLSIGMSMIDSIYYLLINPKLSLRFEKANLIFGIHVPLNI
                     ELFNTDITKNNNTFSFRTKDWDEWQDYLKILRYLQVGGSEDTFYFSIGSNFAQTIGHG
                     SILKRYVPNHDSTFSTLSTKINWYAKYGGFELLLGDVARGNIFGGLAFVKPFSGLGGY
                     HAESASFGYTFVADRNAPTSLKYVNYYDMPDPNIKQPENERHPWDIENDTHYGGERVR
                     RIDTDSKNRPVVTDDMFLYMQGIDFDFKIFKTEDKKVDIKTFLDYSWFKNDFERGGLT
                     LGFLGRFNLDEKNQHGIRTQLEFRVHHDSYLPSFFDTFYDIERMEMLTNIYGKSGFQP
                     DGRSKYYHLMNDSSDKWVFSFYGEFTYSWKTWMALSLGYEQLPSSFSLFAHFELPDLW
                     ILKTMLSYYRRGIDSPSAIFKEDRVSSLFRATVRLQVLPILYINGYVGKHWSFWSADN
                     PRPDDNLEGHYISSWDWGFDLEFGYEWGRVGKGKKKDKKKDSADELLKKEE"
     gene            complement(5739..6344)
                     /locus_tag="PL056_02030"
     CDS             complement(5739..6344)
                     /locus_tag="PL056_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HPM45825.1"
                     /translation="MKNENIQVSQGLLVKSLIISFFLLAAAFTAGLLMGRNDHGQIER
                     TSADQGDVQEKLSDCSYRLQEITAKHISMTDMAREKGILDENGRYNQNIVCAVPEPPK
                     KTEDVNTEKEDTAVAEKIVEKQENKKCLYSIQIFSDPSRDLALSAQKRYDIPKTRLVE
                     GIVNGKNWYRIRSGCYSTRSEAEIDLPLIKEIVDSALVVAE"
     gene            complement(6346..7770)
                     /locus_tag="PL056_02035"
     CDS             complement(6346..7770)
                     /locus_tag="PL056_02035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPM45826.1"
                     /translation="MDSESRLSLLFVLESSPEDGNSIAFLNSYIDSLINLLADETDVT
                     LFYPEFSDTEEHYSLNIIQGSKYKKMVTYLPARHESFKDTFANSRMENIFSYILKEEH
                     FDCVHIWSFKNHSFNYLFIAKERMLPVLVTVHDGFLFSNSVFSKGFPADHENDKVRIS
                     NFVNSSVTVFFKKMLNAVKKRSTCSSWFENIGRYSRYYNKTNLNPVDSFVFEERASLC
                     EDVIKLTDRFQFFSQIEYNLFYRNIIPETQVFFLEQGIENDISFANRPFEIEGAVKFG
                     FIGEILPEEGVLELVEAFNILYEDGYHNEMHFYGELHENHHYFTRLKKKVRNPNVYFH
                     GPIQPGRLNSVLNTFDVLMIPSKWHRSDSFLVNNAISGRKAVIVSNRNNISEKIRRSN
                     RGLILEQVDRVNIATAVSELERNRKRLYYFMRTTDDFKNSDIEDDVCTLVDMYSNYSR
                     SRENFDRVLLKRKLIKKKIDRQRG"
     gene            complement(7760..8971)
                     /locus_tag="PL056_02040"
     CDS             complement(7760..8971)
                     /locus_tag="PL056_02040"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase/3-isopropylmalate dehydratase large
                     subunit family protein"
                     /protein_id="HPM45827.1"
                     /translation="MGKTIIEKIFYNHLLDKNEPVTPGSIVWINIDYKTARDFGGANV
                     VNNLQTSFTGELVFDKKNTMFTFDCVAPAKTIPYAVNQQICRNFARKEGLKVYDVDAG
                     IGSHILIDSYAKPGMTVVGTDSHFNIMGAISCFGQGMGDVDIAYIWKTGRTWFEVPES
                     VKLTVKGKRNDNITAKDIVLFMLKKFGSRTFLGKVVELYGDEMEKLTLDERVTIASMG
                     TEMGVISIMIPTKGLAGLEIIEADKDANYEKEYEIDLDELTEPQLAAPPYPHNVKGLK
                     EFNGKKIDTVFIGSCTNGRYSDFEEVAEIVKGKTIKTNGYIVPATKEIYGRMLKEGLI
                     ATLFEAGFVIANPGCGGCASGQIGMTGDGEVIVSTSNRNFKGKQGNGEVHLASPATAA
                     MSALNGEISGF"
     gene            complement(9060..10076)
                     /locus_tag="PL056_02045"
     CDS             complement(9060..10076)
                     /locus_tag="PL056_02045"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HPM45828.1"
                     /translation="MKRTAGFTTSVPVEVILAAGFKPLDLNNIFVSAQNPYDLVEKAE
                     VEGFAGSSCAWIKGLYSVMTGGFLDPLTDVFIAVTEGDCSNAKVLEQIVSMKTGIRSF
                     IFNYPYERDAAKMSDEIDRFAKFMGTTLEKCESVRSSLSSLRKKLKYIDELSYKFPGI
                     IKGVENHLFLVSSSDFNGDPVLFEKNVDDFLKTVEERKSGFKEKNIKKIAYLGVPPIV
                     PIHEFIEKKGAVVIYNEIQREFAMLDECDSLALQYTNYSYPYSARFRFEKALSEIKKR
                     KPDGIIHYVQSFCHRQLEDIILRTALSELKLEAPVLTIEFDKPAGNVDARVATRIEAF
                     LETI"
     gene            complement(10073..10858)
                     /locus_tag="PL056_02050"
     CDS             complement(10073..10858)
                     /locus_tag="PL056_02050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HPM45829.1"
                     /translation="MFIDSHAHIHMLVKEKGCQISEIIDEMKKNSVGAVMNICGNEDE
                     LIFSQQIRSAFSDSGISFFSVAGFHPHEAEKYIDSPTRWISDNSDSITAVGEIGLDFH
                     YDFSPRETQRKMLRKMIDLSLELQKPLIIHGRDGEDEIIETLLEYGLKDKKVLFHCYT
                     GSLETAMKIFKNGWYLSFSGIVTFNRSIELQKILENSPVDRIFFETDSPFLAPVPFRG
                     KVNTPAKVRYVYEFASRFLNIEVSGMQKKVKDNFERFLNIKMP"
     gene            complement(10860..12146)
                     /gene="ricT"
                     /locus_tag="PL056_02055"
     CDS             complement(10860..12146)
                     /gene="ricT"
                     /locus_tag="PL056_02055"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HPM45830.1"
                     /translation="MEKNNRNINRNIDISEEEFRKLQELEVNDEGFKDEENPKHKKAG
                     SPIAPLGGGNAVYFDYSDEFPAFEDVIAIQFQNAGKVYWYYYPEQKNQGNELKQGDVI
                     VAYSERGMELADVLNKSADEFKRQNKINFIKNGFLRKARPDDLEKAKRLEKNAEQAWE
                     TIERLNKELGIKMKIIKVKYTLDDSKAIFYFSSSGRVDFRELIKRLAYELRRRIEMRQ
                     IGVRDTTKIMGGLGPCGMDLCCSRHMHNFIPVSIKMAKDQNLSLNPNKISGICGRLFC
                     CLGYENPSYEEMRKDYPEEETRIVEKASGREGVVRKINVITKNLTVFFSENYDRKEKN
                     EEKTFPKEALRKENGKWVIIPVATPVESSLSKDSKNIIPFIAKEKELLLGEKRSGERS
                     EENGENQEKKKSGDNHKKFNRNRKFRKNFNQKKENS"
     gene            complement(12130..13011)
                     /locus_tag="PL056_02060"
     CDS             complement(12130..13011)
                     /locus_tag="PL056_02060"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45831.1"
                     /translation="MSQKTVKDNILNAVNKKNLPQTILFYGPSDKSIFNLVNSFAQTL
                     IKPKREIDFQKFIDLCSSQSFPDFITLSKGSTGSIKIEDVLALDNIICYQPYESDNRI
                     IFIRDAGTLTPQAQNALLKKIEEPPARTYFILSANKKNSLLPTIVSRSIAIFVPPEKS
                     DNGEITPFDFFTFLEVLGEEIGLDSVMAEKKKMEMETMNINPNSLGWIDKMAEIITDI
                     SNMAGSTNDVSTAEKTEYHKRMYIRMRLAFFSFYIKNRFPDVSLRIAQFLKNNQYFSF
                     DASVFYNIIGDEIGKEQ"
     gene            complement(13061..13990)
                     /locus_tag="PL056_02065"
     CDS             complement(13061..13990)
                     /locus_tag="PL056_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45832.1"
                     /translation="MKFVRRIVPFTTMILTDAGNFISIIEQSLKWCSSDSFNPQGKVY
                     VIDCDTEKTAVVNCGGVSYSEPSENFSLFKFIWTVKPSITIDFCNTNESKLLMMLSRS
                     GRTIASKSLFSFFFSSTSYKGSLANDLQQAAPYLFNDPGNERFGYRTTSIVDKTVECE
                     CIENRTESDKEEELIIDLGTIPTDPSGKVLWIPKRTTPKGMWNDFISLTYFAQQHNCL
                     IDVHFKDEAILRKFSAIFFIKGRKMDFDKYLNIITSDTADEIIFSNGSINYKFIVFSF
                     GFTPEYRSLYFKLKKAGENERIATIAKARKGLI"
     gene            complement(13987..14445)
                     /gene="nusB"
                     /locus_tag="PL056_02070"
     CDS             complement(13987..14445)
                     /gene="nusB"
                     /locus_tag="PL056_02070"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HPM45833.1"
                     /translation="MGLRRRSREKTLQILYSMEFNRKNSVEEAVGIYLKYFNINSSKN
                     CDNETPEEENVFIVYLLNTVSRNLSEIERLIEEAATNWRIERIALIERNILRMSVAEL
                     IDETNGVPFKATINEAIDIAKKFGSKESSSFVNGIINKVADIIDIKSKKL"
     gene            complement(14455..14916)
                     /gene="ribE"
                     /locus_tag="PL056_02075"
     CDS             complement(14455..14916)
                     /gene="ribE"
                     /locus_tag="PL056_02075"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HPM45834.1"
                     /translation="MKTKTFQGELTAKGKKFGIVVSRFNSFVSEALLNGAIDCLERHG
                     AENAEIIKVPGAFEIPFAARKAALTKKYDAIICIGAIIRGNTPHFEYIAAETVKGIAH
                     VSLETGVYISFGVLTVDTIEQAVERAGTKAGNKGFDAALAAIEMLNVTDKI"
     gene            complement(14906..16144)
                     /locus_tag="PL056_02080"
     CDS             complement(14906..16144)
                     /locus_tag="PL056_02080"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020879080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HPM45835.1"
                     /translation="MSYRPELVENIKKAVERIKAGQMVIMVDDEDRENEGDLVIAAQF
                     ANAENINFMAKNGRGLICLALENSIADQLDLPPMVVDNQSRFGTAFTVSIEAKCGVTT
                     GISAADRATTIMSAVKDGAVPTDLARPGHVFPLRAKDGGVLVRTGQTEGSVDLTRIAG
                     LKPAAVICEIMNDNGTMARMPELREFSAKYDIPIITIEDIIAYRLMTESLVEQMADAV
                     LPTKWGGDFNIKVFKSMVDGLEHVALIKGDITTAEPVLVRVHSECLTGDALGSVRCDC
                     GYQLQNAMEIIDREGRGVILYLRQEGRGIGLGNKIKAYHLQDNGMDTVEANIALGFPA
                     DMRDYGIGAQILKEIGVKRIKLLTNNPKKMVSLAGYGLEITDQIPNICGVQEKNIKYL
                     MAKQNKMGHKIFSKGDEHED"
     gene            complement(16157..17455)
                     /locus_tag="PL056_02085"
     CDS             complement(16157..17455)
                     /locus_tag="PL056_02085"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013789090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HPM45836.1"
                     /translation="MTVHLVTGTQWGDEGKGKIVDILTEKVDIVCRYQGGNNAGHTIV
                     VDGVKYDLHAIPSGILREKVISFIGSGVVLDPKQFIEESEKLASKGIDVSPSKLLISP
                     KTAIIMEYHKILDKCREEAKKDNKIGTTGRGIGPAYEDKISRIGIRAIDLLDKDHLRR
                     KIKAALKEKNALFTDLYKKDPFDSEELTLTYSELGLKLKPYIQEPDFSFAGFSKERKI
                     LMEGAQGALLDIDHGTYPFVTSSNTVSGYFGVGCGIPVDRISDNIGLVKAYTTRVGAG
                     PFPTEDHGPDGDELRKTGFEFGTTTGRPRRCGWLDMVALKYIFDMSGFTSIALTKIDV
                     LNNFEKIKIATGYNCKGRKLNGFPASIEMLENIEPVYIEVEGWKEDISKIRKYEELPE
                     KTRKYVELIETMLGVPVSIVSVGPGREETIIRKEFIQLMK"
     gene            complement(17481..19283)
                     /gene="lepA"
                     /locus_tag="PL056_02090"
     CDS             complement(17481..19283)
                     /gene="lepA"
                     /locus_tag="PL056_02090"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018752753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HPM45837.1"
                     /translation="MKNIRNIRNFSIIAHIDHGKSTLSDRLLEMTGSLTEREKKEQFL
                     DDMELEKERGITIKARAVTVLHTYQGEVYELNFIDTPGHVDFSYEVSRALTSCEGALL
                     VVDATQGVEAQTLANVYMAIDNNLEIIPVINKIDLPSADPEKAKNEIIEIIGIDATDA
                     LEVSAKTGFGVRELLDKIVTDIPCPKTDPAMKTRALIFDSWFDAYVGVRMLVRMFDGC
                     IKNGDMIYLMHSNMEYEVIEILRYTPHPVKVNELNAGEVGVIAAGIKTIHDVNIGETI
                     TLKNDISPLPLPGFKPMKQMVFCGIFPVETSKYEELKKAVEKLALNDSSFSHEPENSS
                     ALGFGFRCGFLGMLHLEIVKERLEREFDLELITTAPSVSYKVILNSGEEVFVENPDKL
                     PKQQLIDHIQEPFVKASVFTPAEFIGGLVKLCLDKRGRQKNLTYQTTDRVILEYEIPL
                     SEIVYDFYDKLKSISRGFASLDYELLDYERSNLVKMDILINGDPVDALSVIVHKEKSY
                     YMGRSLVAKMRKVIPRQMYDVAIQAAVGSNIISRETVKAYRKDVTAKCYGGDVSRKRK
                     LLEKQKEGKKKMKQLGNVEIPQEAFLAVLDANEE"
BASE COUNT         5110 a         4218 c         3728 g         6324 t
ORIGIN      
        1 gggatgaaga tgaatcaatt gtcagatgga tgacccactg ggcaactgag aaaaaagatg
       61 tccttgaatt tgttgaagaa attaagagaa tattaaatta gtcgcaaacc tgatcacaaa
      121 cagggcatgg atcttccaca tcctcttcag caggaaaagt ttcagaacag ctcagatttt
      181 ctgaacatgt tgaacattca tcagctttgt cagcattaaa atttgtgcat ggatcatcat
      241 ttgcttcctg ctccataagt gcgagcatag cttctctgca ctttgcctct gttccaccga
      301 acatcggtct agcggcaata ccttcatcac aagtaaaaag cttttcacaa tagactctgg
      361 cggcatcttc ctgtgcatct gcaagagttg tttcgcttcc gcaagaaaca aaaagaataa
      421 ttccgaaaac agcaacgatc aataactttt tcattttttc cctccagaaa taaaaaatcg
      481 aattacgggc ctatattaga gaaaaacaaa gcggtgtcaa attaaatata caaagaattt
      541 tttctgatgt aatattcaat accttcagga agaagaaggg caacacgctc tttatcgtga
      601 atcatctgtt ttatcattgt gcttgaaata tcgggaaggg cggaaccgtc aaatttatct
      661 tttcctcgac cgatcacttt aatgcttttg cagattcgtt ccaattcatt gaattctttc
      721 cacttctccc tgtttttcca attatctgca ccgatgaaga gatgaaaatt atattcagga
      781 tacagtttcg aaaaatgttt taaaacatca attgtaaaac cggaattgat ctcccgctca
      841 gtttcaagaa cttttatcct tttttcaggc cactgctttg ccagaattct gcacatttca
      901 aggcggtgtt caaaagatgt cgtcttgaat ccgaaagggt ggttaaaact cgggattagc
      961 cacacttcat ccattttata tttttcaagt gcgagatcta tcgtcattct gtgggcaagg
     1021 tgagggggat cgaattttcc accgaaaacg gctatatctt tgatattatt actcatattt
     1081 gagaatgaca gggatgtttc ttttaaatga cattgcatta atccttttat tcagagccat
     1141 tacgagtttt tcatcgtgac cggcttttat aacttcatca gggttcattt tgaggtctgt
     1201 cagagcataa atgacagggt ccatgtcggc ataactgaat ccgatctcag attcatcggt
     1261 ctgaccttca aaaagatctg cactcggagc ttttgttata agcgattcag gaataccaag
     1321 atattttgaa attttaaaca catcggtttt ataaagataa ccgactggat ttattgcaga
     1381 agcgctgtct ccaaacaaag taaagtatcc aagttctatc tcgcttttat tccctgttcc
     1441 gaaaactatc gcttttttag ctgatgaata gtcaaaaagg gttatcattc tgacccttgc
     1501 catgatgttg ccaagccgga tatgccttga aatttccgga ttcaccatat tatttgcagc
     1561 aattgcatca acggcggaag ttatttcaat gtgttcatgg tttattccaa gatgatgaac
     1621 cattttcata gcatcttcga cgcttgcacg gcttgaactt ttgtaaggca ttaatattcc
     1681 agttacattc tcttttccaa gggcaagtga cgcaagagtc gccacaaacg cactgtcaat
     1741 accgcctgaa agaccgagaa ctgcttttga gtgaccgttg cggttcattt cattctgaat
     1801 agaattgaca ataaaattaa ctgcttttga ataattaatt tcctggagct gaatcattta
     1861 tttcctcaat gatatctggt ttataagcga tcttctaagt tttattgaac tgaaaaaaca
     1921 gtcgtgaatg atcttttcgg gttcgtcaga tgaattgtat cctgaaacct gaatataatg
     1981 gttttcaatg gtgtcaagag caactttgtt gaaatcagtt gcaacagcat tccggcattt
     2041 ttcaatgaaa tcgaactcgg cactatctga aaatgtgagc gagtccgcaa gacgggaaag
     2101 ccagaatgcg gcaatgatgt tgtcaggtct agacttgaga ggcggagttt catcaagtgt
     2161 ggaataatct gaaacaaaga agcagttatt tttcttttcg aggaccactt cacaattctc
     2221 aaaaagtgaa aagatctctt tcctcgaaaa aaaacggtga gttccctcat ttgagaaaat
     2281 tgtataccag aaacctctgt cagcttttgt ctgggctacg atcgttcctt tgaatgtatc
     2341 cattattccg aatctatctg ctgtttattc tttttggatt tctgcatatc cgaagttaca
     2401 cccacttttg cggcaagatc aagacagtct ttcagaaagg ggatcgtgac ttctttaatg
     2461 ttctttgcgt cgacatcaag acaccgcaga agatatatct ctccggtctt ttcctgcttg
     2521 agcatataca tttttaaagc accgcgatac aatgcctcaa catagtaggg acagaaactg
     2581 atagcttttt tataattgtc agccgccttt tcgtaaagcg cctgttcatc gtacattaca
     2641 ccgagctctt tgtaaggttc acagtaagtg cccttcattt tgatagctga gttgaaatgc
     2701 ttttccgcaa tgtcatactg cttctgaagt ctgtgaacga tacctatgtt cgtttcgaca
     2761 taatgaggaa aagggtagag agcgtcggaa cttacattct tcaattcttc aagtgcctga
     2821 tcatatttgc caagccgtgt gtagagaacg cccagattat ttcttgcatc ggaatattta
     2881 ggatctattg tcatcgcttt ttttagattt tcttcagctt tgtcatactg cccgtcctga
     2941 agatagacca tgcccagatg gttgtaatac tgcggaattt ccggagccgc ttcaatggct
     3001 tttgaaaact cccttgtcgc tttaatgtaa tcgccctgat tccatgcggc aaggcccatc
     3061 tgatagtaac tgtcggcctt tttttcatcg ctgacagttt ttggttcagc cgatttgcac
     3121 gaaaaggaga acagtgccgc agcaacaaga aaaactgaga gaactctttt cattatctac
     3181 tccgcaacga tcttaaagtt tccgcttgct ccgagattgt tgatctcacc tttatgttca
     3241 gcaaacggtg caaattctat tgagctgccc ttttttattc cggttcctgc aacgatcgtg
     3301 aactttatca cttccggatt gggaacagaa ttgtatgcga caacattttc tcctttctta
     3361 aggaatggat ttatctcaag aatcgaatcc tgctgacctt tcataacttt caccgcttcc
     3421 ttgccgttga tgaaaaaaac aaaatcatta gtgagcggct tatcaaaaga gatcgaaatg
     3481 atatattcct ttgaaggttt tgccgattca tcaacgatct tcacccccgg tgcatcgatg
     3541 tgaatgttcc ccgaactgtc gatctttaca ttgcagttct taagctcagt gtccttcatt
     3601 ccggttgctt tcacaccgtt aatgaaaaga tcacctgcaa agatttgaaa aaatcccaga
     3661 acaattaata aaaacaatcc ttttttcaaa acttattcct cctttttcag aagttcatcc
     3721 gctgaatctt tctttttgtc tttcttcttt ccttttccga cacgacccca ttcgtatccg
     3781 aattcaaggt caaaccccca gtcccaagaa gagatgtaat gtccttcaag gttgtcatcg
     3841 ggccttggat tgtctgcact ccagaaactc cagtgtttcc cgacatatcc gttaatataa
     3901 aggataggaa ggacttgtaa cctgacagta gctctgaaaa gtgaacttac ccggtcttcc
     3961 ttgaatatcg cagacggaga gtcgattccc cgtctgtaat atgaaagcat ggtcttgagt
     4021 atccacagat cgggaagttc aaagtgtgca aaaagtgaaa aagaggaggg gagctgttca
     4081 tatccgagcg aaagcgccat ccatgttttc cagctgtatg tgaactcccc gtagaacgag
     4141 aagacccatt tgtctgacga atcgttcatc agatgataat atttcgatct tccatctggc
     4201 tggaaaccgc tttttccgta aatgttggta agcatctcca ttctttcaat atcgtagaaa
     4261 gtatcaaaga aagaggggag ataggaatca tgatgaaccc tgaactcaag ctgggtcctg
     4321 atgccgtgct gattcttttc atcaaggttg aacctgccga gaaatccgag tgtcagtcca
     4381 ccccgttcaa aatcattttt gaaccatgaa tagtcaagaa aggtctttat atcaactttc
     4441 ttgtcctcag ttttaaagat cttaaaatca aaatctattc cctgcatgta gaggaacata
     4501 tcgtcagtca caacaggacg gttctttgag tcggtgtcga tccttctgac ccgttctccg
     4561 ccgtaatgtg tgtcgttttc tatatcccaa ggatgacgtt cattttcggg ttgttttata
     4621 tttggatcag gcatatcgta gtagtttaca tactttaacg aggtcggtgc attcctgtca
     4681 gcaacgaaag tatatccgaa agatgcactt tcagcatggt atccgccaag tcctgaaaaa
     4741 ggtttgacga aagcgagtcc gccgaaaatg ttgcctcttg ccacatcacc cagaagaagt
     4801 tcaaatccac cgtattttgc ataccagttt atttttgtcg acagcgttga aaaggtcgag
     4861 tcgtggtttg gcacatatct ttttagtatt gagccgtgac cgatagtctg ggcaaaatta
     4921 ctgcctattg aaaaatagaa agtgtcctcc gagccgccga cttgaagata acggaggatc
     4981 ttgagataat cctgccactc atcccagtct ttcgtccgga aactgaaagt gttgttgttc
     5041 tttgttatgt cagtgttgaa aagttcaata tttaaaggga catgtatccc aaagataaga
     5101 ttggccttct caaaacgcag agaaagtttc gggttgatca ggaggtaata tatggagtct
     5161 atcattgaca taccgattga aagacctgca ctgttgtaag gcgacaatat ctcttttgag
     5221 ccgatgttgg caagctctcc cttccagaaa actttttcct ctttcggttt ttcatctttc
     5281 ggtgtttcat cttttttctg ttcgtccgcc ggttttgcgg gagtttcagg tgcaggtgca
     5341 attaccggtt ccggttgagt aacgggctca ggttgggcag gaatttcagg tgccggttcg
     5401 ggctgaggag caggagcagt atccggttga ggggttgatg gctgagcagg cggttctggt
     5461 tcaggtgccg gctcagattg aggagttaca gtttgtggtt gttgagcagg agcaggcggt
     5521 tctaccagtt catcctgcgg ataaaaagcc ggaggttctt tcttttcctc tgtcggctcc
     5581 tcttttttct cttctttttc tgttttttct tcgcctgcag gttttacagt ttcttccttt
     5641 gtttcatcat cttgtgcaaa aaggggaaaa aagaaaattg ctgcacaaag agtgataaca
     5701 aaaaatttaa ttttctgcat atttatctcc acactcaatt attctgccac cacaagagca
     5761 ctgtcaacga tctcttttat aagcggaagg tcaatctcgg cttctgatct tgttgaatag
     5821 cagcccgatc ttattctgta ccagttcttt ccattgacta tgccttcaac aaggcgggtt
     5881 ttgggtatgt catatctttt ttgtgctgaa agcgccagat ctctgctcgg atctgaaaat
     5941 atctgtattg aatagagaca ttttttattt tcctgtttct ctacaatttt ctctgcaact
     6001 gctgtatctt ctttttcagt attgacatct tcagttttct ttggtggttc aggaaccgcg
     6061 cagactatat tctggttgta tctgccattt tcatccagta ttcctttttc acgcgccata
     6121 tcggtcatgc tgatgtgctt tgcggtgatt tcctgaagac ggtagctgca gtcgctgagc
     6181 ttttcctgaa catctccctg atcagcactt gttctttcga tctgaccatg atcattcctt
     6241 cccatcagaa gcccggcagt aaaagcggct gcaagaagaa agaacgatat aattaaagac
     6301 tttacaagca atccctgact cacctggatg ttctcatttt tcattttatc ctctctgacg
     6361 gtcgatcttc ttttttatca atttcctctt taacagaact ctgtcaaaat tttcacggct
     6421 tcttgaatag ttgctgtaca tatcaacaag agtacaaaca tcgtcctcga tatcagaatt
     6481 cttaaagtcg tctgtcgtcc gcatgaaata atacaacctt ttgcggtttc tttcaagttc
     6541 tgaaacagcg gttgcaatat ttacgcgatc aacctgttca agaatgagcc ccctgttgga
     6601 tcttctgatt ttttccgaaa tgttgttcct gttggaaact attaccgctt ttctccctga
     6661 aatggcgttg ttgacaagga aggaatcact tctatgccac tttgacggaa tcatgagaac
     6721 atcgaatgta tttagaaccg agttcaatct tccgggttgt atcggtccgt gaaaataaac
     6781 attgggattc cgcacctttt ttttaagacg ggtgaaatag tggtggtttt catgaagttc
     6841 tccataaaag tgcatttcat tgtggtaacc atcctcataa agaatgttga aagcttcgac
     6901 cagctcaagc acaccctctt caggcagtat ctcacctatg aacccgaatt tgaccgcacc
     6961 ttctatttca aaaggtctgt tggcaaaaga gatgtcattt tcaataccct gttcaagaaa
     7021 aaagacctga gtttccggaa tgatatttct gtagaaaaga ttatattcga tctgtgagaa
     7081 gaattgaaac ctgtctgtca gttttatcac atcttcgcaa agagaagccc tttcttcaaa
     7141 cacaaaactg tcaacaggat tgaggtttgt cttattatag tagcggctgt agcgcccgat
     7201 gttttcaaac cacgatgaac atgttgatct tttttttacg gcattcagca ttttcttaaa
     7261 aaagacagtg acagagctgt tgacaaaatt tgatatcctc actttatcat tttcatggtc
     7321 ggcagggaat cccttgctga aaaccgaatt ggaaaaaaga aaaccgtcat gcactgtaac
     7381 cagaacagga agcatccgct ctttagcaat gaacagatag ttgaatgaat ggtttttaaa
     7441 gctccagata tgaacacagt caaaatgctc ttctttaagt atatagctga aaatgttttc
     7501 cattctgctg tttgcgaaag tatctttgaa agattcatgt cttgcgggaa gatatgtcac
     7561 catcttttta tatttcgatc cctgtattat attcagagaa tagtgttctt ctgtatctga
     7621 gaactccgga tagaaaagtg tgacatccgt ctcatcagca agaagattta tgagagaatc
     7681 tatgtaacta ttcagaaaag cgatcgaatt cccgtcttca ggagaagact ccagaacaaa
     7741 aagcagtgac agacgacttt cagaatccac ttatttcacc attgagagcg ctcattgcgg
     7801 ctgttgccgg gcttgcgagg tgaacttcgc cgttgccctg tttccctttg aagtttctgt
     7861 tggatgtgga aacgatcact tctccgtctc cagtcattcc tatctgtcct gaagcgcaac
     7921 cgccgcaacc gggatttgca atgacgaatc cggcttcaaa aagagttgcg atcaaccctt
     7981 ctttcagcat tctgccataa atctcctttg ttgcaggaac aatataacca tttgttttta
     8041 ttgtctttcc tttaactatt tcagcaactt cctcaaaatc actatacctg ccgttcgtac
     8101 agcttccgat gaatactgtg tctatttttt taccgttgaa ttccttcagt cctttgacat
     8161 tatgagggta gggtggagcg gcaagctgcg gctcagtgag ttcatcaaga tctatttcgt
     8221 attctttttc ataatttgca tccttatcag cttctatgat ctcaagcccg gcaagtcctt
     8281 ttgtcgggat cataattgaa ataacgccca tttctgttcc catgctggct atggtaaccc
     8341 tttcatcaag agtcagtttt tccatttcgt cgccgtaaag ttcaacgact ttgccaagga
     8401 atgttcttga accgaacttt ttgagcatga agagaacgat gtcttttgct gtaatattat
     8461 catttctttt ccctttcaca gtaagcttga cagattcagg aacttcaaac cacgtccttc
     8521 ctgttttcca gatataggca atgtcaacat cacccattcc ctgaccgaaa cagctgatag
     8581 cccccattat attgaaatga ctgtctgttc ctacaacggt catcccgggt tttgcataac
     8641 tgtctattaa aatgtgtgaa ccgatgcctg cgtcaacatc gtaaaccttc aagccctctt
     8701 ttcttgcaaa attgcggcat atctgctgat ttactgcata aggaatcgtc tttgccggag
     8761 cgacacagtc aaaagtgaac attgtgtttt tcttatcgaa aacgagttct ccggtaaaac
     8821 ttgtctgaag attgttgacg acattagctc ctccgaaatc gcgtgctgtt ttgtagtcaa
     8881 tatttatcca gacgatcgaa cccggcgtaa ccggctcatt tttgtcaaga agatgattgt
     8941 aaaagatttt ctcaatgatc gtttttccca tgatttccct ctttagtatt taaataacaa
     9001 taaaattcac gcggagataa taacaaacca aatatcattt gtcaaaaaga agtgaaagat
     9061 cagatagttt caagaaatgc ttcgatcctt gttgcgacgc gggcatctac atttcctgcc
     9121 ggcttgtcaa attctatagt taatacaggt gcttccagtt tgagttctga aagagccgtt
     9181 cggagtatta tatcttcaag ctggcgatgg cagaatgact ggacataatg aattatcccg
     9241 tcgggctttc ttttttttat ttccgacagt gctttttcaa atctgaaacg ggcggagtag
     9301 gggtatgaat agtttgtgta ctgcaaagcg agtgaatcgc attcatcaag catggcaaat
     9361 tcacgctgga tctcgttata aatgacgaca gcaccttttt tttcaatgaa ttcatggatc
     9421 ggcactatcg gaggaacgcc gagataagcg atctttttaa tatttttctc cttaaaacca
     9481 ctctttcttt cttcaaccgt tttcagaaag tcatcaacat ttttttcaaa taagactgga
     9541 tcaccgttga aatctgatga gctgacaaga aaaagatggt tctcgacacc tttgataata
     9601 cctgggaatt tataagacaa ctcatcaata tatttaagtt tttttctaag tgatgaaagt
     9661 gaggaacgga ccgattcaca cttttctaaa gttgtcccca taaacttagc aaaacggtca
     9721 atttcatctg acattttagc ggcatctctt tcgtaaggat agttgaaaat gaaacttctg
     9781 atccctgttt tcatggaaac tatctgctca agcacttttg catttgaaca gtctccctcg
     9841 gtcacagcaa tgaaaacatc ggtcaaggga tcaagaaaac cgcctgtcat gactgaataa
     9901 agccctttta tccaagcaca actgcttcct gcaaaacctt caacttcggc tttctcaaca
     9961 agatcataag gattttgagc acttacgaaa atattgttaa ggtcgagcgg tttgaatccc
    10021 gccgcaagga tcacttcaac aggaacagat gttgtaaagc cggcggttct tttcatggca
    10081 tttttatatt aagaaatctt tcaaagttgt ctttcacttt tttctgcatg ccggaaactt
    10141 caatattcag gaatctgctt gcaaattcat atacatatct gacttttgca ggtgtattta
    10201 ccttcccccg gaaaggaaca ggggcaagaa aaggggagtc ggtttcaaaa aatattctgt
    10261 caacaggaga attttctaag attttctgaa gttcgataga tctattgaaa gttacaattc
    10321 cggaaaaaga gagataccag ccgtttttaa atattttcat ggcggtttcc aaagacccgg
    10381 tgtagcagtg aaaaaggact tttttatctt tcagaccgta ttccagcagt gtttcaatga
    10441 tctcatcctc accgtccctt ccatgtatta tcagcggttt ttgtaattct aaagagaggt
    10501 ctatcatttt tcgaagcatt tttctctgag tttctctggg agaaaagtca tagtgaaaat
    10561 caagtccgat ttcacctaca gcggtaattg agtcactgtt gtctgaaatc cagcgggtag
    10621 gggaatcgat gtatttctct gcctcatgag gatgaaatcc agctactgaa aaaaaagaga
    10681 ttccgctatc agaaaaagca ctccgtatct gttgtgaaaa gatcagctca tcttcgtttc
    10741 cgcatatatt catcactgcg cccacactgt tttttttcat ttcatcaatt atttcagata
    10801 tttgacatcc tttttccttg acaagcatat gaatatgggc atgagaatca ataaacattt
    10861 caggagtttt ctttcttctg attgaaattt tttctgaatt ttctgttccg gttaaatttt
    10921 ttatgattat cgccgctctt tttcttttcc tggttttctc cgttttcttc gctccgctct
    10981 ccggatcttt tttcacccag aagcagctct ttctcttttg caataaatgg aattatgttc
    11041 ttggaatcct tagataaact gctttctacc ggagtggcta cagggattat tacccatttt
    11101 ccgttctcct tcctaagagc ttctttcggg aaagtctttt cttcgttttt ttccttcctg
    11161 tcataattct cgctgaaaaa aacggtaaga ttttttgtga ttacattgat cttcctgaca
    11221 acgccttctc tgccggatgc tttttcaact attcttgttt cttcttcagg ataatccttt
    11281 ctcatttctt cataggaggg gttttcatat ccaagacagc agaaaagccg cccgcaaata
    11341 cccgatatct tattgggatt aagagacagg ttctgatcct tagccatttt tattgaaacc
    11401 ggaatgaaat tgtgcatatg cctgctgcag cagagatcca ttccgcatgg gccgagccct
    11461 cccattattt ttgtagtgtc tcttactccg atctgcctca tttcaatgcg gcgtctcagt
    11521 tcgtatgcaa gccgtttgat aagttctctg aaatcaaccc ttccgcttga tgaaaaataa
    11581 aatatcgcct ttgaatcgtc aagggtatac ttaaccttga tgatcttcat ttttatccca
    11641 agctctttgt tgagccgttc aatggtctcc catgcctgtt cagcgttctt ttcaagtcgc
    11701 ttggctttct ccagatcatc aggacgggct tttcttaaaa atccgttctt tatgaaattt
    11761 atcttgttct gtcttttgaa ctcgtcagca cttttattaa gaacatcggc aagttccatt
    11821 ccccgttcac tgtatgcaac gatgacatcg ccctgtttta gttcatttcc ctgatttttc
    11881 tgttcaggat agtagtacca gtaaactttt cccgcattct ggaactgaat ggcaataaca
    11941 tcttcaaaag caggaaattc atcactataa tcaaaataga cagcgttgcc gccaccgaga
    12001 ggggcaatag gggaaccggc tttcttatgt ttggggttct cttcgtcctt aaaaccctca
    12061 tcatttactt caagttcctg taatttcctg aattcttctt cagaaatatc gatgtttctg
    12121 tttatgttcc tattgttctt ttccaatttc atctcctata atgttataaa aaacagatgc
    12181 atcaaacgag aagtactgat tgtttttcag aaactgtgca attctcaatg atacatcagg
    12241 gaatctattt ttaatgtaaa aagaaaaaaa agcaagccgc attcttatat acatcctttt
    12301 atggtattcg gttttttcag cggttgatac atcatttgtg ctaccagcca tattgctgat
    12361 gtcagttatt atttcagcca ttttatcgat ccagccaaga gagttgggat tgatattcat
    12421 agtttccatt tccatttttt tcttttccgc catgactgaa tcaagaccga tctcttcacc
    12481 gagaacttca agaaaagtaa aaaaatcaaa aggggttatc tcgccgttat cacttttttc
    12541 aggaggaacg aatatcgcaa tgctccttga aacaattgtg ggaagaaggg agttcttttt
    12601 atttgcagaa agaatgaaat aggtgcgggc cggcggctct tcgatctttt ttaagagcgc
    12661 attctgtgct tgaggagtca atgttccggc atccctgatg aaaatgatcc tgttgtcgga
    12721 ttcgtagggt tgataacaga tgatattgtc aagagccaga acatcttcga tctttattga
    12781 acctgtagaa ccttttgaaa gtgtaatgaa gtcagggaat gattgcgatg aacagagatc
    12841 gatgaatttc tgaaagtcaa tttcccgttt aggttttata agcgtttgcg caaaagagtt
    12901 gacaagattg aatatggact tatcgctggg tccgtaaaaa agtattgtct gaggaaggtt
    12961 ctttttgttg actgcattca gaatattatc ttttactgtt ttctgtgaca tttagtttac
    13021 ataatatatg ttatgcgaag ttgatgggat gaaacgtcaa tcatattaaa cccttcctgg
    13081 ctttagcaat tgttgcaatc ctttcatttt cccctgcttt cttgagtttg aagtacagag
    13141 atctgtattc aggtgtaaaa ccgaaagaga aaactatgaa cttgtaattg atgcttccat
    13201 tggagaaaat tatttcatct gctgtgtcgc tggttatgat gttgagatat ttgtcaaaat
    13261 ccatctttct cccttttatg aagaatatgg cactgaattt tctgagtatc gcctcgtcct
    13321 tgaaatggac atctataaga cagttatgct gctgcgcaaa atatgttaat gatatgaaat
    13381 cattccacat tcccttcggc gttgtccttt ttggtatcca aaggaccttc cctgaaggat
    13441 ctgtcgggat cgtgcctaga tcaatgatca gttcttcctc tttatcgctt tcagttctgt
    13501 tttcaatgca ctcgcattca acggttttat caactattga tgttgttctg tatccgaatc
    13561 tttcgtttcc cggatcattg aaaagatacg gagccgcctg ctgaagatcg tttgcaagtg
    13621 accccttgta tgaagtgctc gaaaaaaaga atgaaaacaa actcttagat gctatagttc
    13681 ttcctgatct tgatagcatc ataagaagtt ttgattcgtt tgtattgcag aaatcaatgg
    13741 ttatggacgg ctttaccgtc cagatgaatt taaagagaga aaaattctct gacggctctg
    13801 agtagctcac tccgccgcag tttacaacgg ccgttttttc cgtgtcgcaa tcaatgacat
    13861 aaacttttcc ctgaggattg aacgaatcgc ttgaacacca ttttaaagat tgctcaataa
    13921 tagagatgaa atttccggca tcagtaagga tcattgtcgt aaacggtacg atccttctaa
    13981 caaacttcat agttttttgc tctttatgtc aataatatca gctactttgt taatgatgcc
    14041 attaacaaag gaacttgatt ccttgctgcc gaactttttt gcgatgtcaa tagcttcatt
    14101 tatcgtggcc ttaaatggca cgccgttcgt ttcgtctatc agttcagcaa cagacattct
    14161 cagaatattc ctttctatga gggcgatcct ttcaatacgc cagttcgtag cggcctcttc
    14221 gatgagccgt tctatttcag aaagatttct gcttactgtg ttaagaagat aaactatgaa
    14281 aacattctcc tcttcaggag tttcattgtc acagtttttc gaggaattta tattgaaata
    14341 cttaagatat atcccaactg cttcttccac ggagttcttc ctattgaatt ccatactgta
    14401 aaggatttgg agtgtctttt ctctggatcg tcttctaagt cccatgatta attctcaaat
    14461 tttatcagtt acatttaaca tttctattgc tgcaagtgcc gcgtcaaacc ctttgttgcc
    14521 cgcttttgtt ccggcccttt caactgcctg ctctattgtg tctaccgtaa gaacaccgaa
    14581 gctgatataa acgccggttt caagtgagac atgagctatt ccttttactg tttcagcagc
    14641 gatatattcg aaatgaggag tattgcccct tattatcgca ccgatacaga tgatcgcgtc
    14701 atactttttt gtcaaagccg ctttccttgc tgcaaaaggg atctcaaaag ctccaggaac
    14761 ttttattatc tccgcatttt cagctccgtg tctttccaga cagtcaattg ctccgttcaa
    14821 aagcgcttcg ctgacaaaac tgttgaagcg ggaaaccact atcccgaact tctttccttt
    14881 tgctgtaagt tctccttgaa aggtcttagt cttcatgttc atctcccttg gaaaatattt
    14941 tgtggcccat tttattctgc ttggccatga ggtattttat atttttctcc tgaactccgc
    15001 aaatatttgg tatctggtct gttatttcaa gaccgtaacc ggcaagactt accatctttt
    15061 tcgggttgtt cgtcagaagc tttatccttt tcacccctat ctctttaagt atctgggcgc
    15121 ctatgccgta atctctcata tcggcgggaa atccaagagc gatgttggct tcaactgtgt
    15181 ccatgccgtt atcctgaaga tgatatgctt ttattttgtt gccaagccct attcccctgc
    15241 cctcctgtct gagataaagg atcactcccc gtccttccct gtcaattatc tccatggcat
    15301 tctgaagctg atatccgcag tcacacctga cacttccgag tgcatctcct gtcagacatt
    15361 ccgaatgaac ccgcacaagg acaggttcag cggtagtgat atcccctttt atcaatgcaa
    15421 catgctcaag tccgtcaacc atgcttttaa aaacctttat gttgaaatcg ccgccccatt
    15481 ttgtgggaag aacagcatca gccatttgtt caacaagtga ctctgtcata agacggtatg
    15541 caataatatc ttcaatggtt atgataggta tatcgtattt agctgaaaat tccctgagtt
    15601 cgggcatcct tgccattgtg ccgttatcat tcattatttc acatattaca gcggcaggtt
    15661 tcagtccggc gatccttgta agatcaaccg agccctctgt ctgaccggtt cttacaagaa
    15721 ctccgccatc ctttgctcta agcggaaaaa catgaccagg ccttgcaagg tccgttggaa
    15781 ccgctccgtc ctttacagcg gacatgatcg ttgtagccct gtctgcggct gaaattcctg
    15841 tggtcactcc gcactttgcc tctattgaaa cagtgaatgc cgttccgaac ctcgactggt
    15901 tgtcaacgac catcggagga agatcaagct ggtcagcaat gctgttctca agagcaagac
    15961 atataagtcc gcgaccgttt tttgccatga aatttatgtt ttcagcattt gcaaactgag
    16021 cggctattac gaggtctcct tcattttcac gatcttcatc atcgaccatt ataaccattt
    16081 gtccggcttt tatccgctcg acagcttttt ttatattttc gaccaattca ggtctgtaag
    16141 acataataac tctccgtcac ttcataagtt gaataaactc ttttctgatt atcgtctcct
    16201 cccttccagg cccgacggaa actattgaga cgggaacacc gagcattgtc tcaataagtt
    16261 ccacatattt tctggtcttt tcaggaagtt cttcatattt tcttattttg gaaatatcct
    16321 ctttccatcc ctcaacttcg atataaaccg gctcgatgtt ttcgagcatt tctatcgaag
    16381 caggaaagcc gtttaatttg cggcctttgc agttgtatcc tgtcgcaatt tttatcttct
    16441 caaagttgtt gagaacatca atttttgtaa gtgctatcga tgtaaatcca ctcatgtcaa
    16501 agatatattt aagggcaacc atgtcaagcc aaccgcatct tctcggtctt cctgtggtcg
    16561 ttccgaactc gaaacctgtt tttctgagct catcaccgtc aggtccgtga tcttcagtcg
    16621 gaaatggtcc tgcccccact ctcgttgtgt acgcttttac aagtcctatg ttgtcagaga
    16681 tcctgtcgac cggaattccg cagccgacac cgaaataacc tgaaacggtg tttgaagaag
    16741 ttacgaacgg atatgttccg tgatctatgt caagcagtgc tccctgagca ccctccatga
    16801 gtattttcct ctcttttgag aaccctgcaa aactgaaatc gggttcctga atgtagggtt
    16861 tgagcttcaa gccgagttca gaataagtta gggtaagttc ttctgaatca aaaggatcct
    16921 tcttgtaaag gtctgtaaac agtgcatttt tctctttcaa cgccgctttt atctttctgc
    16981 ggagatggtc tttgtcaaga aggtcaatcg ctctgatgcc gatccttgat attttgtctt
    17041 cgtatgcagg gccgataccc cttccagttg ttcctatttt gttgtctttt ttggcttcct
    17101 ctctgcattt atcaagaatt ttatgatatt ccatgattat tgccgttttt ggggaaatga
    17161 gaagtttcga aggtgaaaca tctatccctt tacttgcgag tttttccgat tcctcaatga
    17221 actgtttcgg gtcaaggact acgccgcttc cgataaaact tatcactttt tctctgagta
    17281 ttccggaagg tatcgcatgc agatcatatt tgacaccgtc aacaactatc gtatgtcctg
    17341 cattgttgcc gccctggtat ctgcagacaa tgtcgacctt ttcggtaaga atatcaacta
    17401 ttttaccttt cccttcatca ccccactggg ttcctgtaac aagatgtact gtcatttcgg
    17461 gttctctccg taacaaattt tcattcttcg ttagcatcaa gtactgccag aaatgcttcc
    17521 tgaggtattt ccacatttcc aagctgtttc attttctttt ttccttcctt ctgcttttcg
    17581 agaagctttc tctttcttga aacatcaccg ccgtagcatt tggcggttac atcttttcta
    17641 tatgctttga ctgtctctct tgagattatg ttcgacccga ccgccgcctg aatcgcgaca
    17701 tcatacatct gtctggggat cacttttctc attttagcaa cgagcgatct gcccatgtaa
    17761 taagactttt ccttgtgaac tatgacagaa agagcatcaa ccggatcgcc atttataaga
    17821 atatccattt taacaagatt tgaccgttca tagtcaagaa gttcatagtc gagtgacgca
    17881 aaacctcttg agatggattt aagtttgtca taaaaatcat aaacgatctc gcttagaggt
    17941 atttcatatt caagtattac cctgtcagtt gtctgataag taagattttt ctgccgtccg
    18001 cgtttatcaa gacacagttt gaccaatccg ccgatgaatt cagccggagt gaatactgaa
    18061 gctttgacaa atggctcctg aatgtggtca atcaactgct gtttaggaag cttgtcaggg
    18121 ttttcaacaa aaacctcttc accgctgttg agaatgactt tatatgaaac gctcggagct
    18181 gttgtgatga gttcaagatc aaattcccgc tcaagccgtt ccttaacaat ttcaaggtgc
    18241 agcatcccca gaaatccgca tctgaaaccg aaaccgagag cacttgaatt ttcgggttcg
    18301 tgagaaaagc ttgaatcgtt aagtgccagt ttttccacag cttttttgag ttcttcatat
    18361 tttgatgtct ctacagggaa tattccgcag aacaccattt gtttcatcgg tttgaaaccg
    18421 ggaagcggca aaggagagat atcgttcttc agagttattg tttcgccgat attgacatcg
    18481 tgaatagttt ttatccctgc cgctatgacc cctacttcac cggcattgag ttcgttcact
    18541 ttgacaggat gaggagtata tctgagtatt tcgatcactt catattccat gttcgagtgc
    18601 atcagatata tcatgtcgcc gttctttatg catccgtcga acattctcac aagcattctc
    18661 acaccgacat aggcatcaaa ccagctgtcg aatatcaaag cccttgtttt cattgccgga
    18721 tctgttttgg ggcatggtat atcggtcact attttgtcaa gaagctccct gactccgaat
    18781 cccgttttgg cagaaacttc gagtgcatcg gttgcatcta ttccgattat ttcgatgatc
    18841 tcgttctttg ctttttcagg atctgcactc ggaaggtcga tcttgtttat aacaggaatt
    18901 atttccaggt tgttatctat cgccatgtag acatttgcaa gcgtctgggc ttcgacaccc
    18961 tgagtcgcat caacgacaag aagagctccc tcgcagcttg taagcgctct gctcacctcg
    19021 taagaaaagt cgacatgtcc gggagtgtca atgaaattaa gctcataaac ttccccctga
    19081 tatgtgtgaa gaacagtgac agccctcgct ttaatggtta ttccccgttc cttttcaagt
    19141 tccatatcgt caaggaactg ttcctttttt tccctttctg ttaagcttcc tgtcatttcg
    19201 aggagacggt cggaaagagt gctctttccg tggtcgatat gggcaataat cgagaagttc
    19261 ctgatattcc tgatgttttt cattcaaatt cccacaaggc cgaaaaaatt tcaatctcaa
    19321 caaacaaaaa cgctcggttt tatacaataa cataagagaa attacaagaa tattgtttgt
//
LOCUS       DAOOYB010000019        19299 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_2723_length_19299_cov_9.631470, whole genome shotgun sequence.
ACCESSION   DAOOYB010000019 DAOOYB010000000
VERSION     DAOOYB010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 19299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19299
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2723_length_19299_cov_9.631470"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..562)
                     /locus_tag="PL056_02095"
     CDS             complement(44..562)
                     /locus_tag="PL056_02095"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HPM45838.1"
                     /translation="MRQVIYFSFTMFLLLFFSNCTNKDKLANDLQEKKEVAVDTVLNN
                     WSKKSPNSMNWSDAKKYCENLNEDGHSDWRLPTISELRTLIKNCPATETDGACMVTND
                     CLHDGNCRTSACNGCSGGSDGRYSKFGDTKWLWSSSEPSDITDRAWVVIFKLGLVNYG
                     NKSGTINVRCVR"
     gene            complement(592..2853)
                     /locus_tag="PL056_02100"
     CDS             complement(592..2853)
                     /locus_tag="PL056_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45839.1"
                     /translation="MNPEIPGTNGPVYAMVKHDDYIYIGGVFTFVKNIYASRVARLHI
                     PTNTWESLGNISYSYINALAVDTAGNLYAGVYKINDDGSVTGNVMKWDDSNWTVLGEE
                     MSVATLAVDNSGNLYAGGSFTNAGGVAVNHIARWDGKNWIALGKGVDGAVSSLAVDDN
                     GNLYVGGNFINAGSVVANHIAKWDGNDWSIFGDGVEVGVSSGVEAILLDGNGNLYASG
                     HFVNEEGTASNYIAQWNGIKWSMLESKMSGKNIVGIKCDGTIYISAMTLDNNGNLYAG
                     GCFLSASGVVARHIAKWDGISWSALGNGINDRVYAITFDNAGNLYVAGDFTTAGDMAV
                     GNIVVWNKERWDALGSGINQEVNAIAVDSNGDVYVGASITTTNGLLTKGIAKWNGTDW
                     SALGSGVNGSVYAIAFDNSGNLYAGGDFITAGDVRVNSIAKWNGTDWSALGSGVDGLV
                     YALTFDNIGNLYAGGTFPLAGGVTVGSIARWDGTNWHPVGSGLCYINPNMGGGCYGGV
                     LALEFDNKGNLYAGGSFNRAGALSVNYIAMWNGNYWSTLGSGMNGSVYALALDNSGNL
                     YAGGFFNSAGDVTASYIAQWDGAGWSSLGAGVDGSVSALTFDGKGNLYAGGHFTSAGG
                     IFANRIARWDGSNWSAAGSGIGGSYHSVSTLAVDLSGGLYAGGNFTSAGGKTSLYFAK
                     YLIPDDETTQPDEDTIMPTDEDINIDDDTIYEATDSVAYDSDTILPDEQVSVDADTGK
                     VSSADGCGCTVVF"
     gene            complement(3034..4089)
                     /locus_tag="PL056_02105"
     CDS             complement(3034..4089)
                     /locus_tag="PL056_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006655423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HPM45840.1"
                     /translation="MKILVTGTAGFIGYHVAEHLVNRGDEVVGLDVVNDYYDINLKYS
                     RLENMGIDKEGAVYGKLVKSTNFDNYSFIKLDLKDKESIEDLFKNQKFDKVCNLAAQA
                     GVRYSLTNPDAYIESNIAGFMNILEASRHNGIKHLVYASSSSVYGANEELPFSTHDNV
                     DHPISLYAASKKSNELMAHVYSHLFKIPTTGLRFFTVYGPWGRPDMALFLFTKAILEN
                     KEMEVFNYGEMLRDFTYIDDIVEGVVRVIDNVPRGNVNWNGKKPDPSSSKAPYKIYNI
                     GNNAPVKLMSFIEAIEEKLGKTAKKKLMPLQMGDVPATYADVEDLVQNIGYKPATTIK
                     TGIDRFIDWYLEYYKAD"
     gene            complement(4106..6148)
                     /locus_tag="PL056_02110"
     CDS             complement(4106..6148)
                     /locus_tag="PL056_02110"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPM45841.1"
                     /translation="MTKLINSRPFFYILTLIFSLVSIHVEKSLSFVIAVWSVFAVLVL
                     YFILIEQIRPPLPPLIIVAIVLTGGKASVLMALLFIALPYIVSRLDQIHYLFFALSSF
                     TVLVMTGDFKKPEDFILYILYFLVSVFVWLFHRKDLWQHLVSEKTPASEVPRKIDEIS
                     SSIVEDPFKPLKNYLLRTTAFHGMKIELHLIELMPGGKARKYNSENSFELRGLIHRVV
                     HDRIELAAKTLLAEQEDVPMMSEYNFRLYYPISMFECGSIAMEPEYVLAVDIKIKEKG
                     ENPDNSNVKHELLNSFREIKNDIAELIRQGEAFRQISIEKQKKENLYSGTAGIVDSFS
                     RDSLFQATALAVFNLVPEAGAVFITEQKGDAHIGHAFRIREDSLRSDSVKMENIEEFG
                     KDEITDEKSIHSLMVNGKLDNVYELHGINKRKDNPVFTQKVFEYLNKFENMSVRLITF
                     NNDIKGTVTILTDNSEKFESLRNQTTSIRMIYKVVSSALNNLEMYEKVEELSNVDGLT
                     GLYNRRYFQDMIERMVMESSRTGTPLSMIMLDIDHFKAVNDTYGHKAGDDVIRFLSRT
                     VKNNVRKVDVAARYGGEEFVVLLHNTNVEGAARLAEKIRILVKDSTINADGSHINITS
                     SFGVSSFPSISMSSGDLVKNSDQALYFSKENGRNKVTVYSKDIADSAGEKEKDEEE"
     gene            complement(6132..6413)
                     /locus_tag="PL056_02115"
     CDS             complement(6132..6413)
                     /locus_tag="PL056_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45842.1"
                     /translation="MKNRIFSITAAAVFSAVVLFLIYDTVISYSEISRNGEVIEALEK
                     DSKKIEEEILEMRLKMERFKKDPKAAEAILRKKFEMLRTDQYFINDKTN"
     gene            complement(6410..6820)
                     /locus_tag="PL056_02120"
     CDS             complement(6410..6820)
                     /locus_tag="PL056_02120"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HPM45843.1"
                     /translation="MNLRRKKEREFSIDISSLVDVLFTLLIFFSLTSTFVKESGLKVD
                     LPEASSGAPLMSPEKYEITISQEGAVSLNSVPVSSTVEMKEILSKFDTDMRLRYVMVI
                     KADTATPHGKVTEVLDILKSLRFENIAVGTRSKK"
     gene            complement(6817..8133)
                     /locus_tag="PL056_02125"
     CDS             complement(6817..8133)
                     /locus_tag="PL056_02125"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HPM45844.1"
                     /translation="MIKQGVILASGLGSRLNSEGRNEPKPLMPVGGMALIERVITLMQ
                     SSGIENIVVVLGYRSDQMTEFFKKRKFSGIDTVINDEYNKKNGISLLRSREKLSQEPF
                     LLSMADHIFSNDFFVKFIEKAEPELEKADVLLSIDRDIEGVFDLDDATKVFTEGSLIT
                     KIAKDLGGYNAIDTGLFLCNHRIFDKLDDIYKRTGDVSISEGMKALAQENKFGAVDMT
                     GNLWQDVDTPSMKNEAEERLIDAFIRNEPCADFFSNNFFYKAAHEAAVGFFKKEHFDW
                     RIIDTLFFISAIIISLISIKLEIAFPSIFTVIGSTLIYYLNRLKNSVKPSADEQSGFS
                     LFSYGVNMVISMIPVVLGTNFIAGLIILVFMVATVSSKVTGLDAVIRTQVPELPEEIS
                     AKYMSPSFFALVYLILVVLPVPFVVTAVTGAIFLLFHESGPKGMQK"
     gene            complement(8139..8909)
                     /locus_tag="PL056_02130"
     CDS             complement(8139..8909)
                     /locus_tag="PL056_02130"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HPM45845.1"
                     /translation="MGLIHLKDVATLGNIVIGFSSAILAIEGNIQAACYLIIAAFVFD
                     HLDGAIARWTKMSNQFGKELDVIADHVTYSVCPAFIVYAFYKEINMLLAFVMALVPLI
                     FGCIRHARNTVYDISFPRFWLGTPRPVSAFYIVGVLGTQLSEVIPYYKWITIPVIIWI
                     GYNNLSFRPFLAHHGRKFNKPLKFFMSMAVLVLFLSGVATSMTGKPWLLDCLLFEMLV
                     YTFFTPFAFDPWDMDGYDEYVKNKKAEIARDMKPEHLR"
     gene            complement(8984..10291)
                     /gene="miaB"
                     /locus_tag="PL056_02135"
     CDS             complement(8984..10291)
                     /gene="miaB"
                     /locus_tag="PL056_02135"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HPM45846.1"
                     /translation="MKKFFIQTFGCQMNVYDSERVAVMLSNSGWIHTENASDSDIIII
                     NSCSVRDKPLKKLFSCAGRYLPLKRRKGTLIFIMGCVAQQLGRKIIEKAPFVDGVFGP
                     GAEDLILQVVEKGKYPFVSNDQDLLEREEIFPESSKGSFFEKHVSSITVMHGCNNFCS
                     YCIVPFVRGKEVSRKSSSILQEVDMLIQKGVLEITLLGQNVNSYNDPQTGKDFTDLLY
                     DVAKKDGLQRLRFMTSHPKDFNEKLAAAFTEIPQLMPNLHLPAQSGSDRILNMMNRKY
                     TIADYLKKLDMARKYLPDVALSGDFIVGFPDETEDDFRQTLDLIERVGYDVIYAFAYS
                     PRPMTAASKLMDNISPDVKAERLNRLLDAQKNRMKEVRNRYLNRTVKVLIEKPSPKSR
                     TLMGRNEHNLVTHVVNAVDDDMGRIIDVKVLEVLENTLRGQKT"
     gene            complement(10402..12093)
                     /locus_tag="PL056_02140"
     CDS             complement(10402..12093)
                     /locus_tag="PL056_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HPM45847.1"
                     /translation="MKNTFFLLLTLVAAFLLVSCEAGDTGNFKIRLRLPIEENNNFCG
                     DGAGENDDTTYCIQESDQVLLSIYTSSTPSGEYVYADRKLIRVASSNGGKEEFIRSLK
                     KGIYYRFFVEVTNANEKLKLTGGLDSILYDDAENFEVDIFLGATGDFVRVVKDKNDYD
                     SSLLSYFESYGSKGAAAAPLKNGKLYLSGGYSFDWEMIVSDTMVFDLKNVSSKKAAAL
                     KPGLMDHTASFLDDGSESGKVVVAFGTTEDDSYSNAILLYDPDKDKYRNIGYGDSVTR
                     AKAITIDGDVYITGGCSGDKVSKKIYKVDKTQTVSEYATLQTGRCNHGIADVSYTKED
                     GTFVPRILIVGGSTDEDGEKPVTGDTFAEIVTASSKAITLTDRTDGDSVELLKKGLVS
                     AGATSLVMDDLEIPETVVTLVGGYLMDGEEDNKAPVVSKNLYVLSEKSDGSWVYDVNT
                     SSDGCARPSVGTIGSAEKSPAKYAAVNCGAGQTARTDKVASDQIIFVVQVKRSLDPEL
                     GVNVFGASIKDSLFKNNSDSENGVIVDGPVAVNELGQAYIFGTQFVYQVSSYALP"
     gene            complement(12130..12441)
                     /locus_tag="PL056_02145"
     CDS             complement(12130..12441)
                     /locus_tag="PL056_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45848.1"
                     /translation="MKLIAKLITAVLIVSFAVPAYAQEKAQQTGINLKVGESFYFEGS
                     IKDKAADEKEESMAEIQRTPFYKSTWFITVVSVVVIAGAAVGIYYGTRTEEPEGNVVE
                     Y"
     gene            complement(12573..12968)
                     /locus_tag="PL056_02150"
     CDS             complement(12573..12968)
                     /locus_tag="PL056_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45849.1"
                     /translation="MFVFPGFNSNIDHRNKKYHIQTEVNRAEERSRINTVVYLSGMIY
                     FSVSSELNGDDALNRESAVSTIRKQHNKVIRDLISDQLAPQRSARDKDSVDFLGMYGN
                     DRMFSCRNKTVFGAQDIFRELLIIPEDEK"
     gene            complement(12972..13238)
                     /gene="rpoZ"
                     /locus_tag="PL056_02155"
     CDS             complement(12972..13238)
                     /gene="rpoZ"
                     /locus_tag="PL056_02155"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HPM45850.1"
                     /translation="MARVTVEDCLEKVPNRLELIILAAKRTRQLLEGAEPLISNTRGN
                     KEPIVALREVSAGKILLKTTEGKFKKNFRKRRRITKYFRNQDED"
     gene            13429..14469
                     /locus_tag="PL056_02160"
     CDS             13429..14469
                     /locus_tag="PL056_02160"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011936954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HPM45851.1"
                     /translation="MSLKVGIISAGSWGTALATLMAEKDYNVTIWAKEQEIVDSINTV
                     HENSLFLKGIKLSEKIKAVSDIQSAVVSMDIVIVASPSQYTRELLSGISFMISPGTYI
                     VLASKGIENGTLKMMYQIAEDILPATLHKNIFVISGPTFAAELAMKMPTCAVIAGHNS
                     EDMGSIQAIFSSPYMRIYRSNDITGVELGGALKNIFAIAVGILEGMGFGKNTQAALIT
                     RSIAEITRLVIAMGGNPLTISGLSGIGDLVLTCTGDLSRNRQVGIRLGKGEKIDAILK
                     SMLMVAEGVTTTKAAYDLSKKVNVEMPITETLYRVLYENADLYKSFQALMTRSLKDEL
                     LSYGGFNDSSDT"
     gene            14450..15190
                     /gene="dapB"
                     /locus_tag="PL056_02165"
     CDS             14450..15190
                     /gene="dapB"
                     /locus_tag="PL056_02165"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HPM45852.1"
                     /translation="MIRVTLNGASGRMGRAITRIAEETGKIVVAHKVDVTEGFEKIEE
                     ITPNDVDVVIDISSPEGFKKALMWAVRNRKPFISGATGLQIADLISLDTAAESIPVLH
                     TSNLSKGVNILFEIMKKAAALTGDADIEIIETHHSKKKDAPSGTALEIGKIITSGQST
                     RNLKSVEGRSGITGERASNEIGYHSIRCGDVPGEHTVIFAYDGERIEISHKALTRDIF
                     ARGAIEAALFIAGKKPGRYSMKDVIGKG"
     gene            15315..16457
                     /locus_tag="PL056_02170"
     CDS             15315..16457
                     /locus_tag="PL056_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005770051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPM45853.1"
                     /translation="MALKREFVFPDNSFFLFGPRGTGKSTILKESFNDALYIDLLENE
                     NFRTYSAYPERLGGTVMAHPEKKIIIIDEIQKVPDLLSQIHLLMETHKEKLFIMTGSS
                     ARKLKKDGTNLLGGRAFKKTMHPFTASELGDLFKIEEALNFGLIPVVFFSNDRYEALS
                     AYVDLYVREEVMMEGLTRNIGNFSRFLESISFSHSCQINLANISRECQIERKTVESYV
                     NILEDLLIAQRIPVFTKKAKRNLSVHPKFYFFDTGLYRTLRPSGPLDKDTEIGGPALE
                     GLVFQHLRAFCDLHRADSKIYFWRTKSGNEVDFVLYGEDLFAAIEVKSSVNLNPYDFN
                     GLKSFGEDYEIAKRILVYRGNDRFVKNGVLVVPATEFLFDPGKFVL"
     gene            complement(16502..17815)
                     /locus_tag="PL056_02175"
     CDS             complement(16502..17815)
                     /locus_tag="PL056_02175"
                     /inference="COORDINATES: protein motif:HMM:NF021622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HPM45854.1"
                     /translation="MKKQLLTIKEFGFIGKSENKEAKKDETGNIYLPKTEFKSLEDFV
                     LKAREDDEYGCEYLRLGFKKGLDKILTAKNYVGIIETKNHIIEILPKIYKNENDDDNT
                     RKIFLKMLKSLRNLPFRHFNTASLESEKLHLFEIFVRMFLEELGKLVRSGIKSQYVLN
                     EENLNVVKGKIKWSEQIRHNLVHKERIFVEYDEYSVNRPENRIIKTTIEFLARKGFSN
                     NTSRLIRQYQFIFDEIPASFNIEKDFLLCKNNRLVKDYLNLLPWCRVFLKNESFSTVT
                     GRNDVFSLLFPMEKVFESYVADEIRKNIGDGYIVSTQDKSHNLFVDLEKFALRPDIVI
                     SKGEEKIILDTKWKVIESISESGRNNISQADLYQMFAYGKKYGAKTLFLIYPENEKFQ
                     IYPENEKFQKSCHLKYEKNDLDLYIFPWKFSSNEKESNIGNLINI"
     gene            complement(17812..>19299)
                     /locus_tag="PL056_02180"
     CDS             complement(17812..>19299)
                     /locus_tag="PL056_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPM45855.1"
                     /translation="EIIKENSPSKKRRQTLQEVFKHFKEKGQIEFITFHQSYSYEDFV
                     EGIKPIPPKEDENQSEEMVFRTQPGVFKRICDEAIIENFLEDYSGKKFYRIALGRKNE
                     NPTERLKYCYEKNLIGLGYIGYKKDLSNYKNEKEIKENANVDPTGYKMLNYLVNGMKN
                     GDIVFVTEGQNIVAICEINGNYSFSENPDEIMIGYEHQRAVKWIKKDINLSVKKLFSY
                     KTVPGNSLSPIIFEKKDVVSNFVLIIDEINRGNISKIFGELITLIEDDKRLGEENEMT
                     VTLPYSKDSFGVPTNLHIVGTMNTADRSIALMDMALRRRFSFQEMMPRYDVLEGIKVE
                     KDGKEIIISELLKKINERIEFLYDRDHTIGHAYFIKLKNNPDYKELCSIFANKIIPLL
                     QEYFYEDWEKIQIVLGDHIKQYKKEEKDECKEWDDEINKTRFIQSKISVETDVIGFNH
                     NDFENSTVYRINPKLIENDMPEESFRKISEPAKKSAEEQDSKTKQ"
BASE COUNT         5588 a         4240 c         3509 g         5962 t
ORIGIN      
        1 gcactgatct tttttgattc gcaaagtgac aaacgcagtg acatcaacgg acacagcgga
       61 cattgatcgt accacttttg ttgccgtagt tcacaagacc gagtttgaaa atcacgaccc
      121 atgcacggtc tgttatatct gatggctccg acgacgacca taaccatttg gtgtctccaa
      181 atttggaata tcgcccgtca gatccacctg agcagccatt gcaggcagaa gtccggcaat
      241 taccatcatg taaacaatca ttcgtaacca tgcaagctcc gtccgtttca gttgcagggc
      301 agtttttgat aagtgttcta agttcagata ttgttgggag ccgccaatct gaatggccgt
      361 cttcatttaa attttcacaa tatttctttg catcactcca attcatgctg tttggcgatt
      421 tctttgacca gttattcaaa accgtatcaa ccgcaacctc tttcttttcc tgcaagtcat
      481 tagcaagttt atctttattc gtacaattac taaaaaataa taaaagaaac atcgtaaacg
      541 aaaaatatat gacctgcctc atatcttcct ctccaaaaaa ttaaattctg cttaaaacac
      601 tacggtacag ccacagccat cagcactact gactttgccg gtatcagcat caaccgatac
      661 ctgttcatca ggcaagatcg tgtcgctgtc atacgctaca ctgtcggtgg cttcgtaaat
      721 tgtatcatcg tcgatattga tatcttcatc ggtcggcata atggtatctt catcgggttg
      781 agttgtttca tcatcaggaa taaggtattt cgcaaaataa aggcttgttt tgccgcctgc
      841 actggtgaag tttcctcccg catagaggcc gccgctaagg tcaacagcca aagtggagac
      901 actgtgataa ctgcctccta taccactccc tgcagcgctc cagttactgc catcccatct
      961 ggcaatacgg ttcgcaaaga taccgcctgc acttgtaaag tgtcctcccg catagagatt
     1021 ccctttgccg tcaaaggtaa gggcagacac gctaccgtct acaccggccc cgagtgaact
     1081 ccagccagca ccatcccatt gagcgatata agatgcggtt acatcgcctg cactattgaa
     1141 aaatcctcct gcataaagat ttccgctgtt gtcaagagca agggcataaa cagatccgtt
     1201 cattccgctt cccagtgtgc tccagtaatt accgttccac atggcaatat aatttactga
     1261 caacgccccg gctctattga atgagccacc tgcatataag tttcctttgt tatcgaattc
     1321 aagagctaaa acgccaccgt agcaaccacc acccatgtta ggatttatat aacacaggcc
     1381 gctcccaact ggatgccagt tcgtaccgtc ccatcgagcg atagaaccta ccgtgacacc
     1441 acccgcaaga ggaaatgttc ctcctgcata aagatttccg atgttgtcaa aagtaagcgc
     1501 ataaacaagt ccatccaccc cactacccag tgcactccag tcagtgccat tccacttggc
     1561 gatagagttt acacgaacat cgcctgcagt gataaaatcg ccacctgcat aaagatttcc
     1621 gctgttgtca aaggcaatgg cataaacaga tccgttcact ccacttccca gtgcactcca
     1681 gtcagtgcca ttccatttgg caatcccttt tgtcaacagt ccgtttgtag tggttatgct
     1741 tgcacccaca taaacatcgc cattggaatc aactgcaatg gcattcactt cttggttaat
     1801 gccgcttccc aatgcgtccc agcgttcttt attccacaca acaatattgc caactgccat
     1861 gtcacctgca gtggtgaaat ccccagccac ataaaggttg ccggcattgt caaaggtaat
     1921 tgcgtaaaca cgatcattaa taccgttccc taaagcactc cagcttatac catcccactt
     1981 ggcgatatgt cttgcaacaa cccccgatgc tgagagaaag catcctcccg catacagatt
     2041 tccgttgttg tcaagagtca tggcagaaat atagatagtc ccgtcacatt ttataccgac
     2101 aatattttta ccactcattt tgctttccag catgctccat ttgataccgt tccactgtgc
     2161 aatataattt gaggcagtgc cttcttcgtt gacaaaatgt ccactcgcat aaagattccc
     2221 atttccatca aggagtatgg cttcaactcc actagaaaca ccgacttcga caccatcccc
     2281 gaatatactc cagtcgttac catcccattt agcaatatgg tttgcaacga cgcttcctgc
     2341 gttaataaag tttccgccca cataaagatt tccgttgtca tccacagcaa gagaagaaac
     2401 cgcaccatcc acaccttttc ccagtgcaat ccaatttttg ccatcccatc tggcaatatg
     2461 gtttacagca acaccacctg catttgtaaa agatccacct gcatagagat ttccgctatt
     2521 gtcaacggca agggtggcaa cagacatctc ctctcccaga acagtccaat tactgtcgtc
     2581 ccacttcatt acatttcctg taacagaacc atcatcattt attttatata ctccggcata
     2641 taagtttccg gcggtatcaa cagcaagagc attaatatat gagtatgaaa tatttcctaa
     2701 cgattcccat gtgttcgtcg ggatgtgaag ccgtgcgact ctacttgcat atatgttttt
     2761 cacaaaagtg aaaactccac cgatataaat atagtcatca tgtttaacca ttgcataaac
     2821 cggaccattg gttcccggta tctctggatt catagctatc catctttcat caccaggcaa
     2881 tgcgtatgct gtcatattta aaaaaattag aaatagcgca ataaagatta aaaaaattcc
     2941 ccgcaagact tcaccccagt tccgattgaa aatattatat tgaatttaat ccaaaatgca
     3001 catattttta ctccacaaaa ggcactctga tcactaatct gctttataat actcaagata
     3061 ccagtcgatg aaccggtcaa ttcctgtttt gatggtggtg gctggtttat agccgatgtt
     3121 ttgaacgagg tcttcaacat cagcgtatgt tgcggggaca tcgcccattt gaaggggcat
     3181 gagtttcttt tttgcggttt ttccgagttt ttcctctatt gcttcaatga atgacatcag
     3241 ttttacgggt gcattgtttc cgatgttgta aattttataa ggtgcttttg aagatgacgg
     3301 atcgggcttt ttcccgttcc agttaacatt gccacggggc acattgtcaa tgacccttac
     3361 aacaccttca acaatgtcat ctatatatgt gaaatctcta agcatttcgc catagttaaa
     3421 aacttccatc tctttatttt caagtattgc ttttgtgaaa aggaacaacg ccatgtcagg
     3481 tcttccccac ggtccgtaaa ctgtgaaaaa acgaagtccg gtcgttggaa ttttgaaaag
     3541 atggctgtaa acatgcgcca tgagctcgtt tgatttcttc gacgcggcat acaagcttat
     3601 cggatgatca acattgtcat gagttgaaaa cggaagctcc tcgttcgctc catacacact
     3661 tgaacttgat gcatagacaa gatgctttat cccgttatgc cttgacgcct caagaatgtt
     3721 catgaagccg gcaatgttgc tttcaatata ggcatcagga ttcgttaagg aatatctcac
     3781 tcctgcctga gcggcaagat tgcacacctt gtcaaacttc tgatttttaa aaaggtcttc
     3841 tatcgattct ttgtctttaa gatccagctt tatgaatgaa taattgtcaa aatttgtgct
     3901 tttaacaagt ttcccataaa cggctccctc tttatcaatg cccatgtttt caagccggct
     3961 gtatttgagg tttatgtcgt aataatcatt cacaacatca agcccgacaa cttcatcgcc
     4021 tctgtttacg aggtgttctg caacatgata accgatgaat ccggcagttc cggtaacaag
     4081 aattttcata tttcctctct gatgattatt cttcttcgtc tttttctttt tcaccggcag
     4141 aatcagcgat atccttcgaa taaacagtca ctttgttacg accgttttcc ttggagaaat
     4201 agagtgcctg atcagaattt ttaacaagat ctccgctgct catggaaatt gacggaaatg
     4261 atgatactcc gaaagatgat gtgatgttta tgtgcgaacc gtctgcattt atcgttgaat
     4321 ctttgacaag tatcctgatc ttttcagcaa gccgtgcagc accttcgaca tttgtattgt
     4381 gaagaagaac aacaaactcc tctcctccgt atcttgccgc cacatcgacc tttctcacat
     4441 tgtttttcac agtccttgaa aggaagcgga tgacatcgtc ccccgcttta tgaccgtaag
     4501 tatcgttgac tgctttgaaa tgatctatgt caagcattat catcgaaaga ggagtccccg
     4561 ttctgcttga ttccatgacc attctttcga tcatatcctg aaaatatctt ctgttgtaaa
     4621 gccctgtcag tccgtcaaca tttgaaagct cttcaacctt ttcatacatc tcaaggttgt
     4681 taagcgctga agaaacaact ttatatatca ttcttattga tgttgtctga tttctgagtg
     4741 actcgaactt ctcgctattg tcggtaagta tcgtgacagt ccctttgatg tcgttgttga
     4801 aagttataag ccggacagac atattctcaa atttattaag atattcaaac actttctgag
     4861 taaacaccgg attgtccttt cttttgttta ttccgtgaag ttcatagaca ttgtccagct
     4921 tgccgttaac catcagtgaa tgaattgact tttcatctgt tatttcatct ttcccaaact
     4981 cttcgatatt ttccattttt accgaatcac ttcttaagga atcctctctt atcctgaaag
     5041 cgtgacctat gtgggcatct cctttctgtt cggttataaa tacagcacct gcctcaggca
     5101 cgaggttgaa tacggcaaga gctgttgcct gaaaaagaga atcccggctg aaagaatcaa
     5161 ctatccctgc agttccgctg taaagattct cctttttctg tttttcaatg gatatctgtc
     5221 tgaatgcctc tccctgtctt atcagttcgg caatatcgtt ctttatctct ctaaaactgt
     5281 tgagcaattc gtgttttaca tttgagttat caggattctc ccctttttcc tttatcttta
     5341 tgtcaactgc aaggacatat tcaggttcca ttgcgattga gccgcactcg aacatgctta
     5401 tcggatagta caagcggaaa ttatattcag acatcatagg aacatcttcc tgttcagcaa
     5461 gaagcgtttt tgccgccagt tcgatcctgt catgaacaac cctgtggatc agacctctga
     5521 gctcaaaact gttctcactg ttgtattttc tcgcctttcc gcccggcata agctcaatga
     5581 gatgaagttc tatcttcatc ccgtgaaaag cagtcgttct tagaagatag ttcttaagcg
     5641 gcttaaaagg gtcttccacg attgaactgc ttatctcatc tatttttctt ggaacttcag
     5701 aagccggtgt tttttcagaa accagatgct gccacaggtc tttcctgtga aaaagccata
     5761 caaatactga tacaagaaag taaagtatat agagaatgaa atcttcaggt ttcttaaaat
     5821 caccggtcat cacaagaaca gtaaatgatg acagtgcaaa aaagaggtag tgtatctggt
     5881 cgagccggct gacaatgtaa ggaagtgcta taaaaagcag agccatcaga acgctcgcct
     5941 tgccgccggt caggactatc gcaactatta tgagcggggg cagtggcggc cttatctgtt
     6001 caatcagtat aaaatacagc acaagaacag caaacacgct ccatactgca ataacaaagc
     6061 tcaagctctt ttcaacatgg atggacacca gtgaaaatat cagcgtgaga atgtaaaaaa
     6121 acggtcttga attaattagt tttgtcattt ataaagtact gatcagttct taacatctca
     6181 aatttctttc tcagaatcgc ttcagcggct ttcggatctt ttttaaacct ttccatttta
     6241 aggcgcattt caagtatctc ttcttctatc ttttttgaat ccttttccag cgcttctata
     6301 acctcgccgt tccttgatat ttcggaatat gaaattaccg tatcatatat cagaaaaaga
     6361 actactgccg aaaaaacagc agcagcagta atagaaaaaa tcctgttttt catttcttcg
     6421 acctcgttcc tacagcgata ttttcaaatc tcaatgactt caatatatca aggacttccg
     6481 tcaccttgcc atgaggagta gcagtatccg ccttaattac cataacatac cttaaccgca
     6541 tatctgtgtc aaattttgaa aggatctctt tcatttcaac agtcgaactt acaggaacac
     6601 tgttcaaact gaccgcacct tcctgactta tggtgatctc atatttttca ggagacatca
     6661 acggggcacc tgatgaggcc tcaggaaggt cgacttttaa acccgattct ttaacgaatg
     6721 tgctcgtaag cgaaaaaaag ataaggagtg taaaaagaac atcaacaagt gagcttatgt
     6781 ctatgctgaa ctctctctct ttctttctcc tcaaattcat ttctgcatcc ccttcggacc
     6841 ggattcatgg aaaagaagaa atatcgcgcc tgtgacagct gtcaccacaa aaggaacagg
     6901 aagaacaaca agaattaaat aaaccagtgc aaaaaacgaa ggactcatgt attttgcaga
     6961 tatctcttca ggaagttcag gtacttgtgt gcggataacg gcatcaagac ctgtaacctt
     7021 tgacgaaact gttgcaacca taaaaacaag aattatcaga ccggctataa aatttgttcc
     7081 aagaaccacc ggtatcattg atatcaccat gttgacacca tatgaaaata atgaaaaccc
     7141 cgattgttcg tccgctgacg gtttaacaga atttttcaac ctgtttaaat aataaatcag
     7201 tgtcgaacca ataacggtga agattgaagg aaacgcgatc tcaagcttta tactgatgag
     7261 ggaaatgata attgctgaaa tgaaaaacag cgtatcgatt attctccagt caaagtgttc
     7321 ctttttgaaa aatccgactg ccgcttcatg tgccgcctta tagaaaaaat tattcgaaaa
     7381 aaaatctgca cacggctcat ttctgatgaa agcatctatc agccgctctt cagcttcatt
     7441 cttcattgaa ggagtgtcaa catcctgcca gagattgcct gtcatgtcaa cggcaccgaa
     7501 tttgttttcc tgcgcaagag ctttcatacc ctctgaaatt gaaacatcac ctgttctttt
     7561 ataaatatca tcaagtttat caaatatcct gtggttgcaa aggaaaagac ctgtatctat
     7621 agcattatat ccaccgagat cttttgctat ttttgtaatt aaagaccctt ctgtaaaaac
     7681 ttttgtcgca tcatcaagat caaaaacccc ttctatgtct ctatctatcg aaagaagaac
     7741 atccgctttt tcaagttccg gctccgcctt ttcaatgaat ttaacaaaaa aatcattcga
     7801 gaaaatatgg tccgccattg aaagcagaaa cggttcctgc gaaagctttt ctcttgatct
     7861 taaaagagat attccgttct tcttgttgta ctcgtcgtta ataactgtgt ctattcctga
     7921 aaactttctt tttttaaaaa attctgtcat ctgatcgctt ctgtaaccaa gaacgacaac
     7981 tatattttct attcccgaag actgcatgag cgtaataact ctttcaataa gggccatgcc
     8041 gcctacaggc atcagaggtt tgggttcatt cctcccttcg ctgttaagac ggctcccaag
     8101 tccgcttgca agaataaccc cctgttttat cattgttttc acctcaaatg ttccggtttc
     8161 atatcacgcg ctatttccgc ttttttgttt tttacatatt catcgtaccc gtccatatcc
     8221 catggatcaa atgcaaacgg agtaaaaaaa gtgtatacca gcatctcaaa aagaagacag
     8281 tccagaagcc acggttttcc agtcatactc gttgccactc cggataaaaa aagaacaaga
     8341 acagccatcg acataaagaa tttaagaggt ttgttgaact ttctgccgtg atgagcaaga
     8401 aacggcctga atgacagatt gttgtatcca atccagatta ttaccggaat ggttatccat
     8461 ttataataag gaataacttc ggaaagctgt gttccgagaa cgcctactat ataaaaagct
     8521 gagacaggcc ttggagttcc gagccagaaa cggggaaaac tgatgtcata aaccgtattc
     8581 cttgcatgtc tgatgcatcc gaatatcaaa ggaacaagtg ccattacaaa agcaagtaac
     8641 atattaattt ctttgtaaaa agcatatact ataaacgccg gacaaacaga atatgtgaca
     8701 tgatcggcaa tgacatcaag ctccttaccg aactggttgc tcatttttgt ccacctggct
     8761 attgctccat cgagatgatc aaaaacaaat gccgcaatta tgagatagca ggccgcctga
     8821 atatttcctt cgatagctaa aatagctgaa ctgaatccga tgacaatgtt cccgagtgtc
     8881 gcaacatcct taagatgaat aagacccatt tctgtctcct ttttacgaaa aaaatctaaa
     8941 accgcataat aataaaaaaa attgctgaaa aatcactatt tctttatgtt ttttgaccac
     9001 gaagggtgtt ttcaagaact tcaagtactt ttacatctat gatccggccc atatcatcgt
     9061 ctactgcatt tacaacatgt gtcacaagat tgtgctcgtt tcttcccatt aatgttctgc
     9121 ttttagggct cggtttttct atcagaacct ttactgtcct gttcagatat ctgtttctca
     9181 cttccttcat cctgttcttt tgtgcatcca gaaggcggtt aagccgttca gcttttacat
     9241 cgggagaaat attgtccatc aattttgaag cggcagtcat cggccttgga gaatatgcaa
     9301 atgcatagat aacatcatat ccgacccgtt ctatcagatc aagcgtctgc cggaaatcat
     9361 cttctgtttc atccggaaat ccgacaatga agtctccact taacgccaca tcaggaagat
     9421 actttcttgc catatctaat tttttgagat aatcagctat cgtatatttt ctattcatca
     9481 tattcagtat cctgtcagat cctgactgtg ccggaagatg aagattgggc ataagttgag
     9541 gaatctccgt aaacgcagca gccagctttt cattgaaatc tttcgggtgg cttgtcatga
     9601 accgcaaccg ctgcaaacca tcctttttcg cgacatcata aagcagatcc gtaaaatcct
     9661 ttcccgtctg aggatcatta taactgttca cattctgccc gagcagagtt atttcgagaa
     9721 cacctttctg aatgagcata tcaacttcct gaagtatgga tgatgactta cggctgactt
     9781 cctttcctct tacgaaagga acaatacagt aactgcagaa attgttacaa ccgtgcatca
     9841 ctgttatcga agaaacatgc ttttcgaaaa atgacccctt tgagctttcg ggaaatattt
     9901 cctctctttc aagaagatcc tgatcgttag acacgaacgg atatttccct ttttcaacta
     9961 cctgcaaaat aagatcttcc gctccaggac cgaacacacc atcaacaaaa ggagcttttt
    10021 caataatttt acggccaagc tgttgagcaa cacaccccat tatgaaaatc agagtccctt
    10081 ttcttctttt caaaggaaga tatctgcctg cacaactgaa cagttttttc aagggcttgt
    10141 cccttacact gcaactgttt atgattataa tatcagaatc cgaggcgttt tctgtatgta
    10201 tccatccgct gtttgagagc attacagcaa ctctttcact gtcatacaca ttcatctgac
    10261 aaccgaaagt ctgtatgaaa aactttttca cggtatctcc tccagaaaga tctattgaat
    10321 atatccgtca aatacatcat gtcaactcaa aaaaaaacgg ggagaaaaat ccccccgtta
    10381 taaaagttgg aatgaggcag atcagggaag agcgtagctg cttacctgat aaacgaactg
    10441 tgtcccgaaa atataggcct gcccaagctc attaactgca acaggtccgt ctactattac
    10501 accgttctca ctgtcactgt tgtttttaaa cagtgaatct tttattgatg caccaaaaac
    10561 attaactccc agttccggat caagacttct cttaacctgt acaacaaaaa tgatctggtc
    10621 agatgcaact ttatcggttc ttgctgtctg gccggctccg caatttacag cggcatattt
    10681 tgcgggactc ttttcagcgg aacctatcgt tccgactgac ggccttgcac atccatctga
    10741 acttgtgttc acatcatata cccagctgcc atcactcttt tcactgagaa cataaaggtt
    10801 tttgctcaca acaggtgctt tgttgtcttc ctctccatcc ataagatatc cgccgacaag
    10861 agttacaacg gtttccggaa tctcgagatc atccattacc agacttgtag ctccggcaga
    10921 aacgagaccc tttttaagga gttcaacaga atcgccatct gttctgtctg taagggttat
    10981 tgcctttgat gaagcagtca ctatttcagc aaaggtatct ccggtcaccg gcttttcgcc
    11041 atcttcatca gttgaaccgc cgactataag aattctcggc acaaatgttc cgtcttcctt
    11101 tgtgtatgat acatcggcaa taccatggtt gcaacgaccc gtctgcaggg ttgcatattc
    11161 agaaacagtt tgcgttttat caactttata tatctttttg cttactttat caccgctgca
    11221 gccgcctgta atatagacat caccgtcaat agtgatcgcc tttgcccttg ttacagagtc
    11281 gccgtaacct atgtttctgt atttatcctt atccggatca tacagaagta tcgcatttga
    11341 atatgaatca tcttctgttg ttccgaaagc gacaacaacc tttccggatt cggaaccgtc
    11401 atcaagaaaa cttgctgtat gatccatcag tcccggtttg agggcggcag cttttttaga
    11461 ggaaacattc ttaaggtcga ataccattgt gtcggatacg atcatctccc agtcgaagct
    11521 gtaaccgccg gaaagataaa gcttcccgtt tttaagcgga gccgcagccg caccttttga
    11581 accgtatgat tcaaaatatg aaagaaggga tgagtcataa tcatttttat cttttacaac
    11641 tcttacaaaa tcacctgtgg cacctaggaa tatatctact tcaaaattct cggcatcatc
    11701 atagagtatg ctgtcaagac cgccggtaag tttgagcttc tcgtttgcat ttgtaacttc
    11761 aacaaaaaag cggtaataga ttcctttctt gagtgatctg ataaactctt ctttgccgcc
    11821 gttgctgctg gcgactctta tgagcttacg gtcggcatat acatactcac ctgaaggagt
    11881 tgatgatgtg tatattgaaa gaagaacctg atcgctctcc tgtatgcagt aagtggtatc
    11941 atcattttcg ccggcaccat ccccgcaaaa attattattt tcttctatgg gaagtcttaa
    12001 ccgtatttta aaattccctg tgtcgccggc ttcgcatgaa acaagcagaa aagcggcaac
    12061 caaagtcaac agcagaaaaa atgtgttttt cataaaatcc tccaaattgt attcgaaccg
    12121 ttctttagat taatattcaa caacattgcc ttccggctct tccgttcttg tgccgtaata
    12181 tatcccgacg gcagcacctg cgatcacgac gacagataca acggtaatga accatgtcga
    12241 tttgtaaaaa ggagtccttt gaatttcagc cattgactct tccttttcat cggcagcttt
    12301 gtctttgatg ctgccttcaa aatagaaact ttcaccaact ttcaagttga tcccggtctg
    12361 ttgtgccttt tcctgagcat aagcaggaac tgcaaaactg acaataagaa ctgctgtgat
    12421 taactttgca attaacttca tttttctacc tatccaaaaa attgtccatt ttacaccaaa
    12481 aaccatcttc gctaactgct tatcaaattt tgatgaaaca gtcaaacttt ttttaaattt
    12541 ttaaaaagca gcccctcgaa gggattaccg gctcactttt catcttccgg aataattaaa
    12601 agctctctga atatatcctg agcaccgaat acagttttat tacgacatga aaacatcctg
    12661 tcatttccat acattccgag aaaatcaaca gaatctttat ctcttgctga tctttgggga
    12721 gcgagttgat cggaaataag gtctctgata actttattat gctgtttcct tatcgtggaa
    12781 actgccgatt cgcgattaag tgcatcatcc ccgttcaatt cggatgaaac agagaaatat
    12841 atcattcctg aaagatatac aacggtgttt atcctgcttc tttcctcagc cctgttaacc
    12901 tcagtctgta tgtggtattt tttattgcgg tgatcaatat ttgaattgaa acccgggaag
    12961 acgaacattc attaatcttc gtcttgattt ctgaaatact ttgttattct gcgacgtttg
    13021 cggaagttct tcttgaattt accttcagtg gtcttaagca atatcttgcc ggcactcacc
    13081 tctcttaagg caacgatcgg ttctttgttt cctctggtgt ttgaaataag aggctccgcc
    13141 ccttccagaa gctgtcttgt cctttttgca gccagaatta tcaactcaag tctattcgga
    13201 actttttcca aacaatcttc aacagttact cttgccatta caggttcctc ctcaaaaaac
    13261 aaaatcagca ggtattatac aactaaaaac cggaaaatca aataatatca cagacacaat
    13321 taaaaagatc agtatatctt ctaatgttta caaagtgtta tctctaagtt gactaagctg
    13381 tttagcgata gtagaatgcc ccgttggttt tttaacggag gaagattgat gagcttgaaa
    13441 gtcggtatca tttctgcagg cagctggggc acagcccttg caacacttat ggctgaaaaa
    13501 gattacaatg tcactatctg ggcaaaggaa caggagatag ttgattcgat caacaccgtt
    13561 cacgaaaaca gtttattttt aaaaggtata aagctttctg aaaagatcaa agcggtgagt
    13621 gatattcagt cggccgttgt ttcgatggac attgtaattg tcgcatctcc ttcacagtac
    13681 acaagggaac tcctcagcgg gatctccttc atgatctctc cgggaacata tatcgttctc
    13741 gcttcaaaag ggatcgagaa cggaacgctc aaaatgatgt atcagatcgc cgaagatatt
    13801 cttccggcaa ctcttcacaa gaatatattt gttatctcag gacccacttt tgcggccgaa
    13861 cttgcaatga aaatgcctac ttgcgctgtt attgccgggc ataattcaga agacatgggg
    13921 tcgatccagg cgatattcag ctctccctac atgaggattt acaggtcaaa tgacataacg
    13981 ggtgtcgaac tcggcggtgc tttaaaaaac atttttgcaa tagctgtcgg gatacttgaa
    14041 ggaatgggtt tcggcaagaa tactcaggcg gcactcatca caaggtcgat cgccgagatt
    14101 acccgtcttg taattgcgat gggtggaaat cctcttacaa tttcgggact tagcggaatc
    14161 ggcgatcttg ttctgacatg tactggagat ctcagcagga acaggcaggt cgggatccgg
    14221 ctcggcaaag gtgaaaaaat tgatgccatt ctgaaaagca tgctaatggt cgctgaaggt
    14281 gttacaacga caaaagcggc atacgatctt tcaaaaaagg taaatgttga aatgccgata
    14341 acggaaacgc tatacagagt tttatatgaa aatgcagatc tttataaaag ttttcaagca
    14401 cttatgacac gaagcttaaa agatgaactt ctatcttacg gaggattcaa tgattcgagt
    14461 gacacttaac ggtgcatccg gaagaatggg cagagcgata acaaggatcg ctgaagaaac
    14521 aggcaaaatc gttgtcgcac acaaagttga tgttacagaa gggtttgaaa aaattgaaga
    14581 aataacgcca aacgatgttg atgttgtaat agatataagt tctcccgaag gtttcaaaaa
    14641 agctctcatg tgggctgtca ggaacaggaa acctttcatt tcaggggcaa caggtcttca
    14701 gatcgcagat ctcatatcac ttgacacagc cgctgagtca atacctgttc tccatacatc
    14761 taatttaagt aaaggagtca acatcctttt tgaaataatg aaaaaggccg ccgcactgac
    14821 tggtgacgcc gacattgaga tcatcgaaac ccatcacagc aaaaaaaagg atgcaccatc
    14881 gggaaccgca cttgagatcg gcaagatcat aacgagcgga caaagcacca gaaatcttaa
    14941 atccgttgaa ggaaggtcag gaattacagg cgaaagggca tcaaacgaga tcggttatca
    15001 cagcatcaga tgcggcgatg tccccggaga acacaccgtt atttttgcat atgacggtga
    15061 aaggatcgaa atatcgcaca aagcgctcac aagagatatt ttcgcaagag gagcaattga
    15121 agccgcactc ttcatcgcag ggaaaaaacc gggcagatat tcaatgaaag atgtcatcgg
    15181 caaagggtaa aagtcagcac agaaaataat tttcaaactc ttgacaaaat cataaaatcg
    15241 ctgtattttt ggaagtagat attccatttc ggaaggagaa atggaaaaag cgattccatt
    15301 ttggaggtga attcatggct ttaaaaagag agtttgtgtt tcctgataac agtttttttc
    15361 tgttcggacc aaggggaacc ggaaaatcaa cgattcttaa agaatctttc aatgatgctt
    15421 tgtatattga tcttcttgaa aatgaaaatt tcagaacata cagtgcttat ccggaacggc
    15481 ttggcggaac cgtgatggca caccctgaaa aaaagattat tatcattgat gaaatccaga
    15541 aagtccctga ccttttgagt cagatacatc tgttaatgga aacacataag gaaaaattgt
    15601 tcataatgac cggttcaagt gcaagaaagc ttaaaaaaga cgggacgaat cttctcggag
    15661 gacgagcgtt caaaaaaaca atgcatcctt ttaccgcaag tgaactggga gatcttttta
    15721 aaattgaaga ggcacttaat ttcggactga tccctgttgt ctttttttca aatgacagat
    15781 acgaagcact ttcggcttat gtggatcttt atgtccgtga agaagttatg atggaaggac
    15841 ttaccagaaa cattggaaat ttctcaagat ttctggagtc tatcagtttt tcgcatagct
    15901 gccaaatcaa tttggcaaac atttcaagag aatgccagat agaaagaaaa accgttgaat
    15961 cgtatgtgaa cattctggaa gatcttctga tcgcccaaag aattccggtc tttacaaaga
    16021 aagccaaaag aaatttgtca gttcatccca agttttattt ttttgacaca gggctttacc
    16081 ggacattgag accatcaggt ccgcttgata aagataccga gatcggagga cctgcgcttg
    16141 aaggacttgt ttttcaacat ttgagagctt tttgcgacct tcatagagca gattcaaaga
    16201 tctatttctg gcggacaaaa agcggaaatg aagtggattt tgtcctttac ggagaagatc
    16261 tgtttgctgc tatagaagtg aaaagctcag taaacttgaa tccttatgat tttaacggac
    16321 ttaaatcatt tggtgaagat tatgaaatag ctaaaaggat tcttgtttat cgggggaacg
    16381 acagatttgt gaaaaacgga gttcttgttg ttccggcgac agaatttctt tttgacccgg
    16441 gaaagtttgt tctgtgaagt tccaaagtgt caaacgggtt ttatggaaat gaggggtttt
    16501 tttaaatatt tatgaggttt ccgatattac tttctttttc attggaagaa aatttccatg
    16561 gaaatatgta gagatccaaa tcattttttt catacttaag gtggcaactt ttctgaaatt
    16621 tctcattttc aggatatatc tgaaatttct cattttcggg ataaataagg aaaagtgttt
    16681 ttgctccgta tttttttcca tatgcgaaca tctgataaag gtctgcctga gaaatgttgt
    16741 ttctgcctga ttcagaaata ctttcaatca ctttccactt tgtatcaaga attattttct
    16801 cttcaccttt tgaaatcacg atgtcaggtc ggagagcaaa cttttcaaga tcaacaaaaa
    16861 gattgtgtga tttatcctga gttgaaacaa tatagccgtc accaatattt ttccttattt
    16921 catcagcgac ataactttca aaaacttttt ccatcgggaa gagcagcgaa aagacatcgt
    16981 ttctccctgt aactgttgag aaactttcat ttttaagaaa aactctgcac cagggaagaa
    17041 gattcagata gtctttcacc aatcggttgt ttttgcaaag gagaaagtcc ttttcaatat
    17101 taaatgatgc cggaatttca tcaaaaatga actgatactg cctgataagc cggctcgtgt
    17161 tatttgaaaa acccttcctt gccagaaatt ctattgttgt tttaataatc ctgttttccg
    17221 gtctattcac agaatattca tcgtattcaa caaaaatcct ttctttgtgg acaaggttat
    17281 gcctgatctg ctctgaccat ttgatttttc cttttacaac attgagattt tcttcattca
    17341 ggacatattg agatttgatt cctgacctca caagctttcc gagttcttca aggaacattc
    17401 tcacaaagat ttcaaaaagg tggagttttt cactttcgag cgatgctgtg ttaaaatgtc
    17461 taaacggcag atttctcaat gatttcaaca ttttaaggaa tatctttcga gtgttgtcat
    17521 catcgttttc attcttataa attttgggaa gaatttcgat aatgtgattt tttgtttcaa
    17581 tgattccgac atagtttttt gctgtcagaa ttttatcgag ccctttttta aaaccaagac
    17641 ggagatattc acaaccatat tcatcatctt cacgagcttt aaggacaaaa tcttctagtg
    17701 atttaaactc tgtcttgggc agataaatgt ttcctgtttc atcttttttt gcttctttgt
    17761 tttcagattt gccaataaat ccgaactctt ttattgtgag caactgcttt ttcattgctt
    17821 tgttttgcta tcttgttctt cagcggattt ctttgctggc tctgatattt tcctaaaact
    17881 ttcttctggc atatcatttt caattagttt gggattaatt ctgtaaacag tagaattctc
    17941 aaaatcatta tgattaaagc cgattacatc tgtttcaact gaaatcttgc tctgaataaa
    18001 tcttgttttg tttatttcat cgtcccattc tttgcactca tctttttctt ctttcttata
    18061 ttgctttatg tgatctccga gaacaatttg gattttttcc cagtcctcat agaaatattc
    18121 ctgaagcaag ggtatgattt tattagcaaa aattgagcaa agttctttgt aatctggatt
    18181 gtttttcagt ttgataaaat atgcgtgtcc aattgtgtgg tcacggtcat aaaggaactc
    18241 aattctttca ttaatctttt taaggagttc gcttattatg atttcttttc cgtctttttc
    18301 tactttaatt ccttcaagaa catcatatct tggcatcatt tcctgaaaac tgaaccttct
    18361 tctaagtgcc atatccatca aagcaattga gcgatctgcg gtgttcattg ttccaacaat
    18421 atgaaggttt gtaggaacac cgaaagaatc ttttgaatag ggaagagtaa cagtcatctc
    18481 attttcttcc cccaaccgtt tatcatcttc gattagagtg atcaattctc caaatatttt
    18541 tgagatgtta ccgcggttga tttcgtcgat tatgaggaca aagttagaaa cgacatcttt
    18601 tttttcaaag attattggac tcaaagaatt tccaggaacc gttttgtaag aaaacaactt
    18661 ttttacgctt aagtttatat ccttttttat ccatttaacg gccctttgat gttcataacc
    18721 aatcataatt tcatcaggat tttctgaaaa agaataattc ccgtttattt cacatatagc
    18781 gacaatattc tggccttctg tcacaaaaac gatatcccca tttttcatac cattaacgag
    18841 ataatttaac attttgtatc cagttggatc tacattagca ttttctttta tttctttttc
    18901 atttttatag ttgcttaaat cctttttata tccgatgtat ccaagtccga tgagattttt
    18961 ctcataacag tattttaatc tttctgttgg gttttcattt tttctaccta atgcaatgcg
    19021 ataaaatttc ttcccagaat aatcttcaag gaaattttca attatagctt catcacaaat
    19081 tcttttaaaa actcctggtt gagttctaaa aaccatttct tcagactgat tttcatcttc
    19141 ttttggagga atcggtttta ttccttcaac aaaatcttcg taactatatg attgatggaa
    19201 ggtaatgaat tcaatctgac ctttttcttt aaaatgttta aaaacttctt gcagtgtttg
    19261 tcttcgtttt tttgaaggac tattttcttt tataatttc
//
LOCUS       DAOOYB010000020        19165 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_2758_length_19165_cov_12.546311, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000020 DAOOYB010000000
VERSION     DAOOYB010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 19165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19165
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2758_length_19165_cov_12.546311"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            51..2138
                     /locus_tag="PL056_02185"
     CDS             51..2138
                     /locus_tag="PL056_02185"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HPM45856.1"
                     /translation="MKDICKAGIEQMKPKLIEGAKVTKFASDIHFVQFEGRFVKIDDA
                     ENELMQDFNGLITLADIISKHLSKGDTGVFNRLLSLIIRLNKANLFDKECASVLKGVS
                     KPEKMFRGCEKKIGTLLPKGLCAFKGKIISSIPGIIFLAVISSASLYFPQLKGLNILK
                     EFTGGVLPSEYAYLAAIAFIWITVSIVLSGISLSSGAALTALGMPAPVTAVFRYGMIY
                     LSVSPVPVISKGRKEAAKHYIMLMLIPFFIAGIAAVLWHFGILRQAMAIVHVTAFSIG
                     IWRVSPLIRSPFTMIAGFFIKGDGSTSTFLRRRFLKDLLTTRKNSAETDRLIFLSTLG
                     LIWLYGIYEYFWHVANSTLSYLLADALSATGMTFVLICISLLFIVLPLILSATGALIV
                     VLGNLRSVASTPLSRMRRLADSITSRNVPAKAQIVAFLAQIPLFSGLDETELAALCSH
                     IRLHKFIAGRKIIQQGDKGDSFYTIVSGSADVVIEDASGRERSVETLSTGDSFGETAL
                     LEDIPRTASIISKGSVAVFEISRESFEKFIVASAGGKEKVTDMIRFGKLLMSNPMFSF
                     MSPKELSHVIMKFKNEKFAAGKLFFDQGDKGDKFYLIKEGTVHIRRSEDGKTVIDKEL
                     HQGDFFGEIALIKEVPRTAKATAVSDCTVATLTKEEFLEIIGHSIFSGRELDSVMRER
                     ASQLGKEALRSCL"
     gene            2129..2542
                     /locus_tag="PL056_02190"
     CDS             2129..2542
                     /locus_tag="PL056_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45857.1"
                     /translation="MLMKLIGIITSLVILNQATGIGAEQMEYLTNIVKITLTQSETDS
                     ISYIVYTDKVMGNENSLPYLSEEEWAEYIRKQMQSKAKNRDTSKDLWIRSYEILETDP
                     VPGRDGRGFVVRSSGPDGSFETKDDIVSGYVYRLE"
     gene            2613..3188
                     /locus_tag="PL056_02195"
     CDS             2613..3188
                     /locus_tag="PL056_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45858.1"
                     /translation="MKKLMFAAVFVLILSGNVYAQGSKKIEMYVAPTFGMGFNFDAYN
                     AVGLLAGVDVIFKVWENNKKAPGKMFAGFDTGFEYWIPTRSTGIYTNYKTHYLDWPVT
                     GYFSYEFKVDAGPLAYAGPFFTAGVGFAVVIDDWDSRAIDNNTDFFVVFSGSFGGHLV
                     FTNNWALKQSFTWGGGALGWGSFNLEAEYRF"
     gene            3185..>3869
                     /locus_tag="PL056_02200"
     CDS             3185..>3869
                     /locus_tag="PL056_02200"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HPM45859.1"
                     /translation="MKFLYFSISFLLFSVILFFVLYFSVDLPVNNLSQKESSVVYDRN
                     GEMIRVFTKDGFFYLPYNENDNIPEKLKKAVLFYEDRKFEHHFGVDLSAIVRAAYQNI
                     KKGKIHSGASTVTMQLCRLMTQKKRSFKNKITESIQALKIERKWNKDVILRKYLTYCP
                     YGQNIYGYKTASLKYFRKPAEDLNWSEAALLAILPNAPGLLHPEKNRERLTNKRNDLL
                     KRMMDAQVIT"
     assembly_gap    3870..3969
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3970..4709
                     /locus_tag="PL056_02205"
     CDS             <3970..4709
                     /locus_tag="PL056_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45860.1"
                     /translation="LAWVGSHNFFDDYNNGQVDALTSRRPVDGLLQPFLYALAIDKLN
                     LELSSEISDTKQSYGNYTPTNIDGKYSGKTSAYKALREFRNVPAVHLLHNFGIEEFYT
                     FLKEAGAKSIPQPPEFYGYSLISGGFDLQPVEIITLYNGLLGNGKFAKLKTIQGEQTK
                     ENELISAKAALDVVSALSKHEIKGQLIPYVATCNRNGRECWTVVLEQDHIVLVWGGSM
                     TGISKPDTASTLNELRTLAFSIYNAIE"
     gene            complement(5033..5638)
                     /locus_tag="PL056_02210"
     CDS             complement(5033..5638)
                     /locus_tag="PL056_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45861.1"
                     /translation="MQKKTESDQNIKYEIIKKECVLPFKCALDIRIQNRITDQQLREL
                     ALKIKETLSLEEKKAAKIFIAYWLPGMILNAGSWARTDFTPSLEVQIFGITKEQEKVF
                     KPQPANYEGKVIGQWLHNQLQMKCVFLEKKDGLFMQSTYSDGSKGENKLIKKKVKGGI
                     RYYEKDNDSGEYYMINSNGNLEAYDTDGFIETYTFTKLKDQ"
     gene            complement(5824..6066)
                     /locus_tag="PL056_02215"
     CDS             complement(5824..6066)
                     /locus_tag="PL056_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007652636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HPM45862.1"
                     /translation="MTTLTNKTFSNVAKKISFTTEMLIVFLVDGRIIEIPLDYFPSLR
                     DANETQRNKWRFIGGGIGIHWEEIDEDISVENLLKG"
     gene            complement(6047..6283)
                     /locus_tag="PL056_02220"
     CDS             complement(6047..6283)
                     /locus_tag="PL056_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017293691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HPM45863.1"
                     /translation="MPTVLRHNGFRFFFFSNENDEPLHIHVEKGESYAKFWLKPVSIA
                     RNIGFNQSEITEIKKILFEKQSDIEEKWNDYFNK"
     gene            complement(6390..8231)
                     /locus_tag="PL056_02225"
     CDS             complement(6390..8231)
                     /locus_tag="PL056_02225"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HPM45864.1"
                     /translation="MRLKNLRSWSVLTALNVAAALILYLFFQNDLMSLTQGNDKLFIF
                     RVTLAAVIFAVLLLWTTGMVLILLLNFRSINYFASMFSAIFFIFLFIDVQVFVTTGMH
                     LYSSFVIGNLMQNNIFNELHFEANTVIAFIMVFIGVFFVNGLITFLTLKLADFKISKY
                     IGNLFVAVFAVSFAGILIFFLMNSEKISKNQGVETVPFFSGTYSFLFEKDPADLKKLE
                     VNYPYKTFADIKIEKKPDILMIFVESLRADIFNSEIMPVSYSFAKEKCITSRNHHSAE
                     LSTIYSTFAFLYGLNIHHYYPFHFRKTRSVPIRILKNNGYVTIGAAASNLKDMSIHSD
                     VLYDQFDKYKEFFDNGWKKDRKMVDWIKKQSNDKKEKRPRFYFTFFQSTHHNYYYPPE
                     FEKHVPVIESDFNYLKGTTLKERKIEVKNRYLNAAGYVDSLIGELLEHFKDKIESGEI
                     IIALAGDHGEEFWDIGALGHGKISNNFRSRVPFALCLPGIEHKEVELSSHVDIFPTII
                     SYLNPLSDTKKWSDGISLLGEIPQNRYITVGGFDFPRRSTEVTLINNRGKLFLNKTTD
                     SINPENKFRIIKRTDLEDRPDPNLSKELDWMMDQFAIDMNRFFSGAN"
     gene            complement(8231..10102)
                     /locus_tag="PL056_02230"
     CDS             complement(8231..10102)
                     /locus_tag="PL056_02230"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HPM45865.1"
                     /translation="MNLKAGVVFLKSFFKDLVSLPEIKPWLLLIPLIALDAVFFTIIQ
                     YKHDFNLADHILIVGVIQKILFDSLLFLIIAMLLKFVTRINLPSVIFMIIYLYLIGQD
                     MTVYFFGNTLFEAHFLDLMTWFAIKGFINIYSLFMAAVFVGCSVGVFFIFKKISTHFR
                     FSDLIKYSVLLAILIIVDPTDALIWKSEEHDGKFKGNEDKNVHYILRCKNEQIRYAAK
                     NSAVNFVNEVFLKKSRKNQNILNSAEPLLKDAAKFNIPIGKRNYEKLENRPFSKIVWF
                     ASESISLDLFKKFNDKIPFETGTSFYESDEFITNMMTNYFSSASPTLQALTATFNSHP
                     NYDMILGGLHQNAIGNVLKKQGFETIFLRSASKYYAGENIIFQKFGFKQIIAREYFSQ
                     FPENAEHIYDWGVSDRIMLNKLVDLLEEYKDKKLFVVILGIDTHPPHGRAEYGFRKTE
                     YPETPESYNGFGSAYRFMKSVYNHDYDMAQMLKKIKDRGLFTEDTLIISTADHACPYN
                     NVVKYVPGFKNTNLGRTPLSFFTPAKLPEYDKNILSSQLDLAPSILHLLNIEVPEGYW
                     GESIFSKNKKPQFVGLNRGVIEYRTDTEKMSFNKNSKSKEYSGFIKVFNSVIIKKDN"
     gene            complement(10105..10596)
                     /locus_tag="PL056_02235"
     CDS             complement(10105..10596)
                     /locus_tag="PL056_02235"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HPM45866.1"
                     /translation="MKTKLFIALFLLMVVTPVYANKVAWIDMKKVWESSKEIAAEKKK
                     MEAMYKQSQDSIKAKQNEIMKLQEKAKKEGPMATDEAKAAMLEEYQRKAAEFQQAVQE
                     EERKLAEKDAKAQEEFVEKVKKLAMKVAIQKGYEIVLAKEHVLYIDDKYDITKLVIAE
                     VNK"
     gene            complement(10606..12939)
                     /gene="bamA"
                     /locus_tag="PL056_02240"
     CDS             complement(10606..12939)
                     /gene="bamA"
                     /locus_tag="PL056_02240"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HPM45867.1"
                     /translation="MKKILIVIVLMLLFCSISALTVEDVQVEGNIRTESSVLTRPFTQ
                     GKEFSEEEIDKILKEVIDLAIFQDISVFYSPEKKTLIFKVIEFPIIKEVTVSGNKKID
                     LEDINEVVTVKKNELLDTAKINRNTTNIINLYRDKGYLMASVEADIVRNEERKEVSVG
                     FKINEGKKSRVKKVTVVGNKNLTDQFIKSNLNTQEDGLFSYFQDGGYKKEQLEEDVAR
                     IMYFYSDEGYVNVKVNKPVLTISPDKKDIYVTFVVTEGLRYKAGDLSISGDTLDKGEY
                     PKIKFTQKKGSWYKHTHLIRDVEKIKAMYGDEGYPFVNIYPDRDLNDKDRIVNLDFVI
                     SKGQKANVERIDISGNERSRDKVIRRELVIFEGELYNYTSQRRSEERIKRTGFYDEVK
                     IEIKEGSRPDLVKLLVTVKERKSGTFNVGAGFSTFESFVFNAKIDQNNFLGYGQNLSL
                     SGQASKIKREISLSFYEPYFFDYDLSFSASFYYRYLNYDSSIYEYYADYSQNSYGFSL
                     TFGVPFWTYFRAYTGYRLKKVDISGKTEHQMNYLYRDIFSSSLEFMLSVDTRNDRMFA
                     TKGIYSIASLEIAPKVFGSDEDILKLDFNFRFYQELFWGVVFKSNLEMGYNIHLENKS
                     LPFAERFRLGGMYSIRGYPFFSIGPKHDGNGTSYNVPTDGKDPTSSTYPYVIGGDKKF
                     VMNNELEIPIVKQMRLNFVGFLDVGNTWAKNENMFYINTKKYDEYDLPLGLFWSAGFG
                     IRWVTPIAPLSFEWGFPLTPRPGDPKFQFEFNIKNSF"
     gene            complement(12936..13616)
                     /locus_tag="PL056_02245"
     CDS             complement(12936..13616)
                     /locus_tag="PL056_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPM45868.1"
                     /translation="MNDDRILVLENIKKSYFVEGHRIDVLKGVDLTLRKGEQLSLTGK
                     SGAGKSTLLNIMATLERPDSGNILFNGTDPVKMSDAKLSSFRNREIGIVFQFHHLLPE
                     FTALENVAIPLMLSISKKESLKKAEAMLERVGLSERLNHKPAELSGGEQQRVAIARAL
                     INDPSLLLMDEPTGNLDNETGKMIIELILSIAKERELTTLFVTHNKLFASEMGRMIEI
                     KEGVVYNL"
     gene            complement(13609..14910)
                     /locus_tag="PL056_02250"
     CDS             complement(13609..14910)
                     /locus_tag="PL056_02250"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPM45869.1"
                     /translation="MTEFIRFIGLRYFSQGRKKSTLSLISLISITGLSLGVATLITVL
                     SVMDGLVKKMEQTVLNSTSHSNVYKLLGSFENHEEITKKVLEIEEVTGAGPVVFSEVL
                     ISSGKHISGAILNGVDSENYGTVSDVPSLVTEGNFGCLHDEQKCNYEKKDVTHAEKLD
                     DFLGENEGVLPRIIIGKDLAEKLKVSPGGIVTAVSSKGKRTAGEDAVPVSGSFIVAGI
                     FETGMYDYDSRFAYSNIKDVQKFLQIGSHISFVSMKVKDPSKIDEINRKVMDKAGGFP
                     FAVQDWKEMHKTTFKFLNLQKLIMFIILIFIILVASFGIITTLIMLVISKTREVSVLR
                     SLGAKRSTIAGIFIFDGFIIGLIGTLLGAVLAAVMCIALSRINFPISKEIYFFSTLPV
                     EMSLFSFLSVMVSTMIISIIATLYPSLKATGITPVEGLRYE"
     gene            complement(14903..16210)
                     /locus_tag="PL056_02255"
     CDS             complement(14903..16210)
                     /locus_tag="PL056_02255"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPM45870.1"
                     /translation="MKTESFIGLRYLWNTRRTRFLSVITVICVLGISIGVAALICVQS
                     IMDGLQNQMKKTILGAKAHISVEHIEGEFRDYENIRTEILKHEEISGVTPIISRDVIF
                     SSNDEMIGAVLNGIDPATAETALLLPSQIISGSLECLEEPSTCPAIIEKRMKDPSKIL
                     KRDLENESGKTRYPGIAIGSELAKFYGIDIGDVMKVISPVGGGMGPAGPVPLIRSFQV
                     AAVFYSGLYEYDLNYGYITLKNAADFFSTEDKVDSLGVKLKDVYGVDKPQELIKNMVG
                     DKYTVKNWRDMNRQLFGALKMEKLIWFLILGFIVLVAAFNIVSALVMLVMGKKEEIAI
                     LRAVGFTSASVMKIFMLDGVVIGFLGTVLGTAAGYAICVFLDGLTLSVAQDVYYIDRI
                     PVDMSVLTFVYSSAGSMVLSVIATIYPGRKAASMSPSEALRHD"
     gene            complement(16207..17724)
                     /gene="lysS"
                     /locus_tag="PL056_02260"
     CDS             complement(16207..17724)
                     /gene="lysS"
                     /locus_tag="PL056_02260"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HPM45871.1"
                     /translation="MNSNPVVAQIDENEIIAKKKEKIKALKSEGIHPYANSFSPANNV
                     SQVKEFCSKTAKEELEKTGKDQKFSIAGRVMLLRVMGKLAFANIKDETGTLQVVFAKD
                     FLKDDFDRFKRSYDVGDIVWVEGEPYVTRTGEFSILAHKTELLTKNIRPLPEKFHGLT
                     DKEMRYRQRYLDLIMNDEVRETFKKRVKIISFIRNYMDSEGFLEVETPMMHPLVSGAA
                     ARPFITHHNALDMALYMRIAPECYLKRLLVGGMGKVYEINRNFRNEGMDVKHNPEFTM
                     MEFYWPYATYEDQMNFTEKMVTALVNEVCGSQQVEYQGKMIDFSAPWKRMTIEESIVN
                     TGICTIEQLSDKDKLIEIAVEKGLQKEALLKLSRFKLIMELFDNFVEETLWNPAFITR
                     YPTEVSPLSRRNDENPEYSDRFELFMAGREIANAFSELNDPQQQYEAFAAQVEAKKAG
                     DDEAIDMDNDYIRALEYGMPPAAGQGIGIDRLVMLLTDSASIRDVILFPQMRPEK"
     gene            complement(17721..18014)
                     /locus_tag="PL056_02265"
     CDS             complement(17721..18014)
                     /locus_tag="PL056_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45872.1"
                     /translation="MKKKIIVAVAVVFIAVFAVLVYVSNSGSDSEIKGLEKKIESSMK
                     QNNRIKPGNLKIQKQLDKEKLRKMMKRRKLKEEPNTEEQKTENEKKQTPETEK"
     gene            complement(18125..>19165)
                     /locus_tag="PL056_02270"
     CDS             complement(18125..>19165)
                     /locus_tag="PL056_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45873.1"
                     /translation="QAPVITEDDTVFEWSPVLELNTEYTVKVLDGVEDTAGNLMKTGD
                     GHEWSFKTSAYNIYPVVNFYKVLIPLTKEPLCDSTGNAFNNSSNFEVIDKLTTLAVAG
                     TITCDAGAKTVKFVPATQFAKDGNYTIKIKSNAQYSDSSTIGAVDLIMDFHFEMIIDD
                     DFENGSEGWEMENNWEIGVPNYNAYSYKITTAKSGTKVLATGLTTEHRESASYSAKSK
                     NKFVVEEYDQYYVEFWAYVGLENYMDPQCAMLVKIVEETTPKIESVAMTVWGGADLFG
                     DYWINSAKQPNKAVRGKSVDNTYSFFDGVIEGVTTSSGKQFELWFLLHTDGGYAKAGI
                     YLDDVKLYKTVF"
BASE COUNT         5410 a         3989 c         3592 g         6074 t
ORIGIN      
        1 gtaattgtga gatataaaat aagcaagtaa ccatatttca ggagaatagc atgaaagaca
       61 tttgtaaagc gggtattgag cagatgaagc cgaagctcat tgagggggca aaggtgacaa
      121 aatttgcatc tgacattcat tttgtccagt ttgaaggcag gtttgtaaaa attgatgatg
      181 ctgaaaatga acttatgcag gatttcaatg gtttgatcac tcttgctgat attatttcaa
      241 aacacttgtc aaaaggtgat accggtgttt ttaaccggct tctttctctt ataatccgtt
      301 tgaacaaagc taaccttttt gataaggaat gtgcgtcagt attaaaaggt gtttcaaaac
      361 cggaaaaaat gttccgtggt tgtgaaaaaa agattggaac cctgcttcca aaagggttat
      421 gtgcttttaa agggaagatc atatcctcca ttccaggtat tatttttctt gcggttattt
      481 catcggcttc actctatttt cctcagttga aagggctcaa cattttaaag gaatttaccg
      541 gtggtgttct tccatctgaa tatgcatacc ttgcggcaat agcattcatc tggatcactg
      601 tttcaatagt tctttccgga atatcactct cttcaggtgc cgcattgacg gcccttggaa
      661 tgcctgcacc ggtaacggct gttttcagat atggaatgat ctatctatcc gtaagccccg
      721 ttcctgttat ttcaaaaggg cgaaaagagg cggcaaagca ttacataatg ttaatgctca
      781 tccccttttt cattgcagga attgcggcag ttctgtggca tttcggaatt ttacgacagg
      841 cgatggcgat agttcatgta accgcttttt cgataggaat atggcgggta tcacctctga
      901 taagatcacc attcacgatg attgccggtt tctttataaa aggagacgga agcacttcaa
      961 catttcttag acgcagattc ttaaaagatc ttcttacaac aagaaaaaac tctgccgaaa
     1021 ctgaccggct catttttctg tcaactctcg gacttatatg gctttacggg atctatgaat
     1081 atttctggca tgttgcaaat tcaacactca gttatcttct tgccgatgct ctttcagcaa
     1141 ccggaatgac atttgtgctg atctgcattt cacttttgtt cattgtcctt ccattaattc
     1201 tttctgccac aggcgcactc attgtcgttc ttggaaatct gaggagtgtt gcatcaactc
     1261 cactttccag aatgagacgg cttgcagaca gcataacatc cagaaatgtc cctgcaaaag
     1321 cacagatagt cgcatttctt gctcagatcc ctcttttttc aggacttgat gaaactgaac
     1381 tcgccgcact ttgttctcat atcagacttc ataaattcat tgcgggaaga aagatcattc
     1441 agcaggggga caagggcgac agtttttata ctatcgtttc cggctctgcc gatgttgtta
     1501 tagaggatgc aagcgggaga gagcgatctg ttgaaactct ttcaacggga gattccttcg
     1561 gtgaaactgc actccttgaa gatatcccgc gtactgcaag cataatttca aaaggttctg
     1621 ttgcagtttt tgaaataagc agggagtcgt ttgaaaaatt tattgttgcg tctgcaggcg
     1681 gaaaggaaaa agtgaccgat atgataaggt ttggaaagct tctgatgtca aatccgatgt
     1741 tttcttttat gagcccgaaa gagctttccc atgtcataat gaaatttaaa aatgaaaaat
     1801 tcgcagcagg taaactgttt tttgatcagg gagacaaagg agataagttc tatctgataa
     1861 aagaggggac agttcatatc agaagatctg aagatggcaa aactgttata gataaagaac
     1921 tgcatcaggg cgattttttc ggtgaaattg cattgataaa ggaagttccc cgtactgcga
     1981 aagcaacagc agtttcagat tgcactgtgg caacactcac aaaagaagag ttccttgaaa
     2041 taataggtca cagtattttc agcggcaggg aacttgattc ggttatgcgc gaaagagcat
     2101 ctcaactcgg gaaggaggcc ctcagatcat gcttatgaaa cttatcggga ttataacttc
     2161 acttgtaata ctcaaccagg cgaccggcat cggtgccgag cagatggaat atctcacaaa
     2221 cattgtaaaa attactctca ctcaaagcga aacagattcc atatcctata ttgtctacac
     2281 tgacaaagtg atgggaaacg aaaattcact cccctacctt tcagaagaag aatgggctga
     2341 atacatccgc aaacagatgc agtcaaaggc aaaaaacaga gacacttcaa aagatctctg
     2401 gataaggtct tatgaaattc tggaaactga ccctgttccg ggaagagacg gaaggggctt
     2461 cgttgtgaga agttcggggc ctgacggttc atttgaaaca aaggacgaca ttgtttccgg
     2521 ctatgtctac cgtctggaat gaaaaaactt tgatttttta tcaataaaaa caagaataca
     2581 ttttgagatc agttttttat gaggagggtg gaatgaaaaa attgatgttt gctgcggttt
     2641 ttgtcttgat tctgagcgga aatgtctatg ctcaggggtc aaaaaaaatt gaaatgtatg
     2701 ttgcgcccac tttcggaatg gggtttaatt ttgatgctta taatgcagtc ggtcttcttg
     2761 ccggagtaga tgttattttc aaggtgtggg agaacaataa aaaagcaccg ggaaagatgt
     2821 ttgcaggatt tgacaccggc tttgaatact ggattcccac aagatcgacc ggaatttaca
     2881 caaattataa aacacattat cttgactggc ctgtaactgg ttatttttcc tatgaattca
     2941 aagttgatgc cggacctctt gcctatgccg gaccgttttt tacagcaggt gtcggttttg
     3001 cagttgtaat tgatgactgg gacagtagag caattgacaa taacacagat ttttttgttg
     3061 tcttttcagg gtctttcggc gggcatcttg ttttcaccaa taactgggca cttaaacaga
     3121 gttttacatg gggaggaggc gcattgggat ggggatcttt caatcttgag gcagagtaca
     3181 ggttttgaaa tttctttatt tttccatttc atttctctta ttttctgtaa ttctcttttt
     3241 tgtgctttat ttttcggttg atcttcctgt aaacaatctt tcgcaaaagg agagcagtgt
     3301 cgtttatgac cgcaatggtg aaatgatcag agtttttaca aaagacggtt tcttttatct
     3361 cccttacaat gaaaatgata atatcccgga aaaactgaag aaagcagtcc ttttttatga
     3421 agacagaaaa tttgaacatc atttcggtgt agatctttca gccattgtaa gagcggctta
     3481 ccagaatata aaaaaaggga aaatacacag cggtgcttca actgtaacca tgcagctatg
     3541 ccggctgatg acccagaaaa aaagaagttt taaaaataag atcaccgaat caattcaagc
     3601 acttaaaatt gaaagaaaat ggaataagga tgtgatatta agaaaatatc tcacatactg
     3661 tccatacggt cagaacattt acggatataa aacggcatcg ctgaaatatt tccgcaaacc
     3721 tgctgaagat ctcaactggt cggaagcagc gcttctcgca atacttccga acgctccggg
     3781 acttcttcac cctgaaaaga atagagagcg gctcacaaat aaaaggaacg acctgttaaa
     3841 aaggatgatg gatgcgcaag ttattacaan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnt tcttgcctgg gtaggatcgc acaatttttt tgatgattac aataacggac
     4021 aggttgatgc tctcacttca agaagacctg ttgacggact tcttcagccc tttctttatg
     4081 cccttgcaat tgataaactt aatctcgaac tgagcagtga aatttccgac acaaagcaaa
     4141 gttacggcaa ctatacgccg acgaatattg atggaaaata ttcaggaaag acaagtgctt
     4201 acaaagcatt aagagaattt cggaatgttc ctgccgttca tcttctccac aatttcggaa
     4261 ttgaagagtt ctacactttt ctgaaagaag caggtgcaaa aagtattcct cagccacctg
     4321 aattttacgg atattctctg ataagcggcg gttttgacct tcaaccggtt gagataataa
     4381 ctctttataa cggactgctt ggaaacggaa agttcgcaaa actcaaaacg attcaaggcg
     4441 aacaaacaaa agaaaatgag ctgatatctg ccaaagccgc acttgatgtt gtttccgcac
     4501 tgtcgaaaca cgaaataaaa ggtcaattaa ttccgtatgt tgccacttgc aaccgtaacg
     4561 gaagggaatg ctggacagtt gttcttgagc aagatcacat cgttcttgtc tggggcggca
     4621 gtatgacagg gatttccaaa cccgacaccg cctcaactct gaatgaactc agaacgctcg
     4681 ctttttcaat atataacgct attgagtaag tttcgctttt ttaacttgat aattcaatgg
     4741 gttgcattat actgcaaaag aacgccaaca attgacactc gtttgcagta tggagcataa
     4801 ccgggtcaaa ttcaaatcta atcatatcaa agcgttagat ttttttgcag ggagggcttg
     4861 attgaaaaag gcaactgttt taatgagcga aaacatatgt acttatggcg accaaaattc
     4921 aaatgaattt tattcaacag atctatcagt ggtattttat ccacgaactt cacgaagcga
     4981 catcttattg acaactcatt taagcaagac aaattcaaac ttcttgatta aattattggt
     5041 cttttaattt agtaaatgtg taggtttcaa taaatccatc tgtgtcgtat gcttcaagat
     5101 tgccatttga atttatcatg taatactcac cggaatcatt gtctttttcg taatatctta
     5161 ttccaccttt tacttttttc ttaattagct tgttttctcc ttttgaaccg tctgaataag
     5221 ttgattgcat aaaaagacca tccttttttt caagaaagac acacttcatt tgcaattgat
     5281 tatgtaacca ctgtccaata acctttcctt cataatttgc aggttgaggt ttgaacactt
     5341 tttcctgttc ttttgtaatg ccaaaaatct gaacttcaag tgaaggcgta aaatcagtcc
     5401 gtgcccaaga tcctgcattc aatatcattc ctggtaacca ataagctata aatattttag
     5461 cggctttttt ttcttcgagt gacaaggttt cttttatttt caacgccaat tctcttaatt
     5521 gttgatccgt tattctgttc tgaattctaa tgtctaaagc acatttgaaa ggcaacacgc
     5581 actctttttt aataatctca tatttaatat tttggtctga ctctgttttc ttttgcactt
     5641 gtttttcggt gggctcctcc ttaattcttt ctttcggaac aacaggttga gtaaccgatg
     5701 cattatctta tttgtccttg ttgcatccat taataaaaaa agcaataaat agcagcaaca
     5761 caacaatttt aaatagcgcg tttttctttc tcatcgctga caacctcaat taatgtttta
     5821 aaattatcct ttcaacaggt tttcaactga aatatcttcg tcaatttctt cccagtgaat
     5881 acctattccg ccaccgataa aacgccactt attgcgttga gtttcgtttg catctctgag
     5941 tgaagggaaa tagtcaagcg ggatttcgat gattcttccg tcaaccagaa aaactatcaa
     6001 catttcagtt gtgaatgaga tcttttttgc aacatttgaa aatgtcttat ttgttaaagt
     6061 agtcattcca tttctcctct atatcgctct gtttttcaaa taatattttt ttgatttcag
     6121 tgatttccga ctgattaaag ccgatatttc ttgctattga aacgggcttc aaccagaact
     6181 ttgcataact ttcgcctttt tcaacatgta tatgaagagg ttcatcgttt tcattgctaa
     6241 aaaaaaagaa tctgaatcca ttgtgtctta aaaccgtagg caaagaaccc tccattatta
     6301 aaattcattg aacattataa tttcattgac agaaatatca actacaactt caaaaaaaag
     6361 gagtcatacg ggtaaagaag ccgtaagtct caattagccc ctgaaaaaaa tctgttcatg
     6421 tcgatggcga actggtccat catccagtcg agttcttttg agagattcgg atcaggtctg
     6481 tcttcaagat ctgttctttt gatgatccgg aacttgtttt ccgggtttat tgaatctgtc
     6541 gttttgttta aaaaaagttt accgcggttg ttgataagtg ttacttcagt gcttcttctt
     6601 ggaaaatcaa atccgcctac tgtgatatag cggttttgcg gaatttcgcc aagaagtgat
     6661 attccgtcag accatttttt tgtgtctgac aatggattta agtagctgat aattgtagga
     6721 aatatgtcga catggcttga aagttcgact tctttatgtt caatccccgg aagacacagc
     6781 gcaaaaggaa ctctgcttct gaaattattt gatattttac catgaccaag tgccccgata
     6841 tcccagaatt cctccccgtg atctcctgca agagcaatga tgatttctcc agattctatc
     6901 ttatctttga aatgttcaag aagttcgcct ataagcgaat cgacatatcc cgccgcattg
     6961 agatacctgt tcttcacttc gatttttctt tctttgagcg ttgttccttt aagataatta
     7021 aaatcggatt cgatcacggg aacatgcttt tcaaattcgg gaggatagta ataattgtga
     7081 tgggttgact gaaaaaatgt gaaataaaac cggggtcttt tctctttttt gtcattgctc
     7141 tgctttttta tccagtcaac catttttcta tcttttttcc agccattatc aaaaaattcc
     7201 ttatatttgt caaactgatc gtacaggaca tctgaatgaa tggacatatc tttgaggttt
     7261 gaagcggctg caccgatcgt gacatatcca ttgttcttta atatccggat cggaacagac
     7321 ctcgtttttc taaaatggaa aggatagtaa tgatggatgt taagcccgta aagaaacgca
     7381 aaagtgctgt aaattgttga aagttccgct gaatggtgat tgcgggaagt tatgcacttt
     7441 tctttggcaa aactgtatga aacaggcatt atttcagagt tgaaaatatc agctctgaga
     7501 gattccacaa atatcatcag tatatcgggt tttttctcaa tctttatgtc tgcaaatgtt
     7561 ttgtatggat agttaacttc aagctttttc aagtcagcag gatccttttc aaagaggaag
     7621 gaatatgttc cgctgaaaaa aggaactgtc tcgacccctt gattttttga gatcttttct
     7681 gaattcatta aaaagaagat caatatccct gcaaatgaaa ccgcaaaaac agcaacaaat
     7741 aagttcccga tatatttaga tattttaaaa tctgcgagct ttaaagtcaa aaaagtaata
     7801 agtccgttta cgaaaaacac cccgataaat accataatga atgcaataac ggtgtttgcc
     7861 tcaaaatgaa gttcgttaaa aatattattc tgcatgagat ttccaattac aaaagatgaa
     7921 tacagatgca ttcccgttgt cacgaaaacc tgtacatcaa tgaaaagaaa aatgaaaaaa
     7981 atggcagaga acatggatgc aaaatagtta attgatctga aattaagtaa taatatcaaa
     8041 accatgcctg ttgtccaaag gagaagaacg gcaaatatta cagcggcgag cgtcactctg
     8101 aaaataaaca gtttgtcatt tccctgagtg agcgacatca agtcattttg aaaaaaaaga
     8161 tatagaatta aagccgcagc aacattaaga gcggtcagaa ctgaccacga ccttaaattc
     8221 ttcagacgca ctaattatcc ttctttatta taacggaatt aaaaacctta ataaatccgg
     8281 aatattcttt cgacttgctg tttttattga agctcatttt ctcagtatcc gtcctgtatt
     8341 cgataacacc acggtttagt cccacgaact gtggtttttt attttttgag aaaatacttt
     8401 cgccccagta accttccgga acctcgatat tgaggagatg aaggatgctc ggagcaaggt
     8461 caagctggct tgaaaggatg tttttatcgt attcaggcag ttttgcagga gtaaaaaaag
     8521 aaagcggtgt gcgaccgaga tttgtgtttt taaatcccgg aacatacttt acgacattgt
     8581 tgtagggaca tgcgtgatcg gcagtcgaaa ttataagtgt gtcctcagta aaaagacccc
     8641 tatccttaat tttcttaagc atttgagcca tatcataatc gtgattatat accgatttca
     8701 tgaatctgta tgcactgccg aaaccattgt aactctcggg cgtttcggga tattctgtct
     8761 tcctgaaccc gtattcagcc ctgccgtgcg gagggtgagt gtcaatcccc aggatgacaa
     8821 caaacagttt tttatcctta tattcttcca gaagatcgac aagcttgttg agcatgatcc
     8881 ggtcacttac tccccagtca taaatatgct cggcattttc aggaaactga gaaaaatatt
     8941 cccgtgcgat tatctgctta aacccgaatt tctggaaaat gatattctct ccggcataat
     9001 attttgaagc acttcttaaa aatattgtct caaacccttg ttttttaagg acatttccaa
     9061 tcgcattctg atgaagtcca ccgaggatca tgtcatagtt gggatgactg ttgaaagttg
     9121 cggtaagtgc ctgaagggtc ggagaagctg atgaaaaata atttgtcatc atattagtga
     9181 tgaattcatc gctttcataa aacgatgtgc ccgtttcaaa agggatcttg tcgttgaatt
     9241 ttttaaacag atcaagagag atcgattcgg aggcgaacca gacgatcttg ctgaaaggtc
     9301 tattttcaag cttttcatag tttctttttc cgatgggaat gttgaacttt gccgcatctt
     9361 taagcagcgg ttctgcggaa ttcaggatgt tctgattttt tctgcttttt ttcaggaaca
     9421 cttcatttac aaaatttaca gcactgttct ttgctgcata tctgatctgt tcgttcttgc
     9481 atcttaaaat gtaatggaca ttcttgtctt catttccctt gaattttcca tcatgctctt
     9541 cacttttcca gataagggca tcagtcgggt cgacaataat gagtatggcg agcagaacag
     9601 aatattttat aagatctgaa aatctgaaat gggttgagat cttcttgaaa ataaaaaaaa
     9661 ccccgaccga acaaccgaca aaaacagcag ccataaacag cgaataaatg tttataaacc
     9721 cttttatggc aaaccaggtc atcaggtcaa gaaaatgtgc ctcaaacagg gtgttcccga
     9781 aaaaatatac cgtcatatcc tgcccgatca gatagagata aatgatcatg aaaatgacac
     9841 tcggcaggtt gatacgggtc acaaatttaa gaagcatcgc aatgatgaga aaaaggagcg
     9901 aatcaaaaag aattttctga atcactccaa caatgagtat gtgatcggca aggttgaaat
     9961 cgtgtttgta ctgaatgatc gtgaaaaaga ccgcatcaag cgcaataagc ggaataagga
    10021 gaagccaggg tttgatttcc ggtaaagaga caagatcttt aaaaaaagat ttaagaaaga
    10081 ccacacctgc ttttaaattc atttttattt gttaacttca gctataacca gttttgttat
    10141 gtcatatttg tcatcaatat acagaacatg ctctttggca agaacaatct cgtatccttt
    10201 ctgaattgca actttcatcg caagtttttt caccttttca acaaattcct cctgcgcttt
    10261 tgcatctttt tcagcaagct ttctttcctc ttcctgaact gcctgctgga attcagccgc
    10321 ctttctctga tattcttcaa gcattgccgc tttcgcttcg tcagttgcca tcgggccttc
    10381 tttctttgct ttttcctgca gcttcattat ttcgttctgc tttgccttga tcgaatcctg
    10441 gctctgcttg tacatggctt ccatcttttt cttttcggca gctatctcct ttgaactttc
    10501 ccacactttt ttcatgtcta tccatgcaac tttatttgca tagactggtg ttacgaccat
    10561 aagcagaaaa agagcgataa ataactttgt tttcataatt ccctcctaaa aagaattctt
    10621 tatgttaaat tcgaactgga acttcggatc acccggtctc ggagtcaggg ggaaacccca
    10681 ctcgaaagag agcggtgcaa taggtgtcac ccatcttatt ccgaaccctg cactccagaa
    10741 aagtcccaga ggcagatcat attcatcata tttctttgtg ttgatataga acatgttctc
    10801 gttctttgcc catgtgtttc caacatcaag aaaacctaca aaattaagcc gcatctgctt
    10861 tacaataggt atttcaagtt cattgttcat cacgaatttt ttgtctccgc ctatgacata
    10921 aggataggta ctgcttgtcg ggtcctttcc gtctgtcgga acattgtagc ttgttccgtt
    10981 gccgtcatgt ttaggcccga tcgagaaaaa gggataacct ctgatgctgt acatacctcc
    11041 aagacggaat ctttcggcaa aaggaagcga tttgttctca agatggatgt tgtaacccat
    11101 ttcgagatta cttttgaaca ctactcccca gaaaagttcc tgatagaatc tgaaattaaa
    11161 atcaagtttg agaatatctt cgtcactgcc gaacactttc ggagctattt caagacttgc
    11221 aattgaatat atgccctttg ttgcaaacat tctgtcgttc cttgtgtcta ctgaaagcat
    11281 aaattccagt gaggatgaaa aaatatcacg gtaaagataa ttcatctgat gttccgtttt
    11341 tccgcttatg tcaactttct tgagccggta tccggtatat gccctgaaat atgtccagaa
    11401 agggacaccg aaagttagag aaaaaccgta actgttctgg ctgtagtccg catagtattc
    11461 gtaaatgctt gagtcatagt tcagatatct gtaataaaat gacgcgctga atgaaagatc
    11521 gtaatcaaag aaatatggct cataaaaact taaactgatc tctcttttta tcttgcttgc
    11581 ctgacccgaa agactcagat tctgaccata tccgagaaag ttgttctgat ctatctttgc
    11641 attgaagaca aaactttcaa aagtggaaaa accggcaccg acattgaaag ttccgctttt
    11701 tctttctttg accgtaacaa gaagtttcac aagatcgggt ctgctccctt cctttatttc
    11761 gatcttcact tcatcataaa aaccggttct ttttattctc tcttcgcttc ttctctggct
    11821 ggtataattg taaagttccc cttcaaaaat aaccagttca cgacggatga ctttgtcgcg
    11881 ggacctttcg ttccctgaaa tatctattct ctctacattt gccttctgtc ctttgcttat
    11941 cacaaaatcg aggttcacaa tacggtcttt gtcgttaagg tcacggtcgg gatagatgtt
    12001 cacaaaagga tatccttcgt ctccgtacat cgccttgatc ttttcaacat ctctgatcag
    12061 atgagtgtgt ttgtaccagg aacccttttt ctgagtgaat tttatcttcg gatactctcc
    12121 tttatcaagg gtgtctccgc tgatactaag gtcgccggct ttgtaacgca gcccttccgt
    12181 tacgacgaaa gtgacataaa tatccttctt gtcgggagat atggtgagaa ccggcttgtt
    12241 gacctttaca ttgacatacc cttcgtcgct gtaaaaatac attatccttg ccacatcctc
    12301 ttcaagctgc tccttcttat agccaccgtc ctgaaaatat gaaaaaagcc cgtcctcctg
    12361 agtgttcagg ttggatttga tgaactggtc ggtaagattt ttgttgccga caactgtcac
    12421 tttcttgact ctgctctttt tgccttcatt tatcttaaac ccgacagaaa cctcttttct
    12481 ttcctcgttt ctgacaatgt ccgcttcgac tgaagccata agatatcctt tgtcgcgata
    12541 aagatttatg atgtttgtgg tattcctgtt tattttcgct gtatcaagca gctcgttctt
    12601 ttttactgtt acaacttcat tgatatcttc aagatctatt tttttgtttc cactcaccgt
    12661 gacttccttt atgatgggaa attcgatgac tttgaaaatt aatgtctttt tttccggaga
    12721 gtaaaaaaca gatatgtcct gaaagatcgc aagatctata acttccttga gaattttatc
    12781 gatctcttct tctgaaaact ctttaccctg tgtgaaagga cgggtgagaa cagatgactc
    12841 tgtcctgata tttccctcta cctgaacatc ttcaactgtg agagcactta tgctgcaaaa
    12901 cagaagcatt aaaactataa ctataagaat tttcttcata aattgtaaac aaccccttct
    12961 ttaatttcaa tcattctgcc catttcagaa gcaaacaatt tgttgtgggt aacgaacaaa
    13021 gttgtcagct ctctttcctt tgcaatggaa agaatgagct ctattatcat ttttcctgtc
    13081 tcattgtcca gatttcctgt aggttcatcc attaataaaa gtgaagggtc gttaataagc
    13141 gctcttgcga ttgcaactct ctgctgttct ccgcctgaaa gctcagccgg tttgtggttc
    13201 agccgctcag aaagtccaac tctttcaagc attgcctctg ctttttttaa agattctttt
    13261 tttgatattg aaagcatgag cggtatagca acattttcaa gtgcggtgaa ttccgggagc
    13321 agatggtgaa attgaaaaac tatcccgatc tctctgtttc tgaaactgct taattttgca
    13381 tcactcattt ttacaggatc tgtcccgtta aaaaggatat taccgctgtc gggcctttca
    13441 agagtggcca ttatgttcag gagtgtcgat ttccctgctc cgctctttcc tgtaagagaa
    13501 agctgttccc ctttacgcaa agtaagatcg acccctttta gaacatcaat tctgtgccct
    13561 tccacaaaat agcttttttt gatgttctca agaacaagta ttctgtcatc attcatatct
    13621 caaaccctcc acaggggtta tccccgttgc ctttaaactt gggtaaagtg ttgcaattat
    13681 tgaaattatc atcgttgaaa ccattactga aagaaaactg aaaagagaca tttcaacagg
    13741 gagagtcgaa aagaaatata tctccttgct tatcggaaaa tttatccttg aaagagcaat
    13801 gcacattact gcggcaagca ctgcaccgag caaggttccg ataagaccga ttataaatcc
    13861 gtcaaaaatg aatatcccgg cgatcgtgct cctttttgca cctaaagatc tgagaactga
    13921 aacctctctc gttttgctta tgacgagcat aatgagggtg gtgatgatgc cgaaagaggc
    13981 aacaagaatt atgaaaatga ggatgataaa cattataagt ttctgtaaat taagaaactt
    14041 gaaagtcgtt ttatgcatct ctttccagtc ctgcactgca aaagggaaac cccctgcctt
    14101 gtccataact ttccggttga tctcatcaat ttttgaaggg tcctttactt tcatcgaaac
    14161 aaaactgatg tgtgaaccga tctggagaaa tttctgtaca tcttttatgt ttgaataggc
    14221 aaatcttgaa tcgtagtcat acattcccgt ttcaaaaatt cctgcaacga taaagcttcc
    14281 gctgacaggt actgcatctt cacctgcggt cctttttcct tttgaactta cagccgtaac
    14341 tatgccgccc ggacttactt tgagtttttc agcaaggtct ttccctataa ttatccgcgg
    14401 cagaacccct tcattttcac cgagaaaatc atcaagtttt tcagcgtgtg taacatcttt
    14461 cttttcataa ttgcactttt gttcgtcgtg aagacaaccg aaattccctt ctgtgacaag
    14521 tgacggaaca tcggaaacag tcccgtaatt ttcactgtca actccattaa gtatcgctcc
    14581 tgaaatgtgc ttccccgaag agatcagcac ttcactgaaa acgacaggac ccgcacctgt
    14641 aacttcttct atttcaagca ctttttttgt gatctcttca tgattttcaa aactgccgag
    14701 aagcttgtag acatttgaat gcgaagttga attcaaaact gtctgctcca tttttttaac
    14761 cagtccgtcc atcaccgaaa gaaccgttat caaggttgcc acaccgagcg aaagtcccgt
    14821 tatcgagatc agagagatga gcgaaagagt gctctttttt ctcccttgag aaaaatatct
    14881 taaaccgatg aaccttatga actcagtcat ggcggagtgc ctccgaaggt gacatcgaag
    14941 ccgcttttct tccgggatat attgtggcga tgaccgaaag taccattgag ccggcagacg
    15001 aatatacaaa agtaaggacc gacatatcga caggtattct gtcaatgtaa tagacatcct
    15061 gtgcgacact gagcgtcagt ccgtcaagga aaacgcagat cgcataaccc gccgcagttc
    15121 cgagaaccgt tcctaaaaaa ccgatcacaa cgccatccag cataaatatc ttcattacag
    15181 atgccgatgt gaacccgaca gcacgaagta tcgcgatctc ctcttttttg cccatgacaa
    15241 gcatgaccag tgctgaaaca atgttgaatg cggcaacaag aacaataaaa ccaaggatca
    15301 gaaaccagat cagtttttcc attttaaggg caccgaaaag ctgcctgttc atgtcacgcc
    15361 agttttttac agtatatttg tctcccacca tattctttat taattcctgc ggcttatcaa
    15421 caccatagac atctttcagc tttactccca gggaatctac tttatcttca gttgaaaaga
    15481 aatcagcggc attttttaaa gttatgtaac cgtaattgag gtcatattcg taaagtccgc
    15541 tgtaaaaaac agcggcaacc tgaaacgacc tgatgagcgg aaccggaccg gcaggcccca
    15601 tccctccgcc gacagggctt atcactttca tgacatcacc gatgtcgatg ccgtagaatt
    15661 ttgcaagttc agaacctatc gcaattccgg gataacgggt ttttccgctt tcattttcga
    15721 ggtctctctt taatatttta gaaggatctt tcatcctttt ttcaattatt gcgggacatg
    15781 ttgacggctc ttccagacat tcaagagagc ccgatattat ctgtgaagga agaagaagcg
    15841 ccgtttctgc agttgccgga tcgatcccgt tgagcaccgc accgatcatt tcatcatttg
    15901 aagaaaagat cacatctctt gaaattatcg gcgtaacacc tgaaatttcc tcatgtttca
    15961 atatctcagt ccttatgttt tcataatctc tgaactcacc ttcaatatgt tcaactgaaa
    16021 tatgtgcctt tgctccgagg atcgttttct tcatctggtt ctgcaatccg tccatgatcg
    16081 actggacaca tatcagggca gctaccccga tcgatatccc gagaacacat ataactgtga
    16141 tcacacttaa gaatctcgtt cttctcgtat tccagagata tcttaaaccg atgaaagatt
    16201 cggttttcac ttctcaggcc tcatctgcgg aaaaaggata acatctctga tacttgcaga
    16261 atcggtaaga agcattacaa gccggtctat ccctatcccc tgtcctgctg caggaggcat
    16321 tccgtattca agtgctctga tgtagtcatt atccatatct atcgcttcat catcacccgc
    16381 tttttttgct tcgacctgag cggcaaatgc ttcgtactgc tgctgaggat cgttgagctc
    16441 gctgaatgcg tttgcgatct cccgacctgc catgaaaagt tcaaatctgt cagaatattc
    16501 aggattttcg tcatttcttc ttgaaagagg gctcacttcg gtggggtatc tcgttatgaa
    16561 agcagggttc cagagagttt cttcaacgaa attgtcaaac agctccatta ttaacttgaa
    16621 tctgctcagt tttaaaagtg cttctttctg aagacccttt tcaactgcga tctctatgag
    16681 tttgtcttta tcagacaact gttcgatagt gcatattcct gtatttacta tgctttcttc
    16741 aatcgtcatc cttttccacg gggcagagaa atcaatcatt ttgccctgat attcaacctg
    16801 ttgagaaccg cacacttcat ttacaagagc ggtcaccatt ttttcagtga aattcatctg
    16861 atcttcataa gttgcataag gccaatagaa ctccatcatt gtgaattcgg ggttatgttt
    16921 tacatccatc ccttcatttc tgaaattcct gtttatctca taaaccttcc ccattccgcc
    16981 tacaagcagt cgtttaagat aacattcggg ggcgatcctc atataaagag ccatgtcaag
    17041 agcgttgtgg tgcgtgatga aaggtcttgc cgcagctccg cttacaagtg gatgcatcat
    17101 aggtgtctcc acttcaagaa aaccttctga atccatataa ttccttatga aagagattat
    17161 ttttaccctt tttttgaaag tctctctcac ttcatcgttc attatcagat cgagatatct
    17221 ttggcggtaa cgcatctcct tgtctgtaag tccgtggaat ttttcaggaa gcgggcggat
    17281 gttttttgtg agcagttctg ttttgtgtgc aaggatagaa aattcaccgg tacgggtgac
    17341 atagggctcg ccttctaccc agacaatgtc ccccacatca tacgatcttt tgaatctgtc
    17401 aaagtcatct ttaagaaaat cttttgcgaa aaccacctga agagtccccg tttcgtcctt
    17461 tatattggcg aaagcgagtt tgcccatcac ccgaaggagc ataactctcc cggcaattga
    17521 aaatttctga tcttttccgg ttttttcaag ctcttctttt gcggtctttg aacagaattc
    17581 cttaacctgt gaaacattgt ttgcaggtga aaaactgttt gcataagggt gtatcccttc
    17641 gctttttaaa gcttttattt tttccttttt ctttgcaatt atctcatttt cgtctatctg
    17701 agccacaact ggatttgagt tcatttttct gtctccggtg tctgtttttt ctcattttcc
    17761 gttttctgtt cttctgtatt cggttcttct ttcagttttc ttcttttcat catttttctg
    17821 agtttttcct tatcaagctg tttctgaatt ttcagattgc ccggctttat ccgattgttc
    17881 tgcttcatgg atgactctat cttcttttcc aaccctttga tttcactgtc tgatcctgaa
    17941 tttgaaacat agaccagaac agcaaatacc gcaataaaaa caactgcaac cgcaacgata
    18001 attttcttct tcatgaaaca cctccctaaa aaaaccccgt taatatatta gtatatgtct
    18061 ctttagtcaa atattgtgat catttcactt tataaacgcg tcttcttttc ctctgtgctt
    18121 tcgtttaaaa cactgtctta taaagtttta catcatcaag atagattccg gcttttgcat
    18181 aaccgccatc tgtgtggaga aggaaccaga gttcgaattg cttaccggat gaagttgtaa
    18241 ccccctcgat tactccgtca aagaaagaat atgtgttgtc aacgcttttt ccgcggactg
    18301 ccttattcgg ctgttttgct gaatttatcc agtaatctcc gaaaagatct gctccgcccc
    18361 atactgtcat tgccactgat tcaatcttgg gtgttgtttc ttcaacaatt ttgacaagca
    18421 tggcacactg aggatccata taattttcaa gtccgacata agcccagaat tcaacataat
    18481 actgatcata ttcttcaaca acgaatttgt ttttgctttt tgcagaatag cttgcacttt
    18541 ctctgtgttc agttgtgagt cctgtcgcaa gaacttttgt tccgcttttt gctgtcgtta
    18601 tcttgtaact gtaggcgttg tagttcggca ctccgatttc ccaattgttc tccatttccc
    18661 atccctcaga tccgttttca aaatcatcgt caattatcat ctcaaaatgg aaatccataa
    18721 taagatcaac ggctcctatt gttgaactgt ctgaatactg agcattgctt tttattttta
    18781 ttgtgtaatt cccgtctttt gcaaactggg tcgcaggaac gaacttaacc gtctttgcac
    18841 cagcatcaca ggttatcgtt ccggcaacgg caagagttgt caatttgtca ataacttcaa
    18901 aatttgagct gttattgaaa gcgtttccag tgctgtcgca taacggctct tttgtcaatg
    18961 gtatcaaaac cttataaaaa ttcacaaccg gatagatatt atatgcggaa gttttgaacg
    19021 accattcatg accgtctccg gtcttcatca gattgcctgc cgtgtcttca actccgtcaa
    19081 gcactttgac tgtatattca gtattaagtt caagaaccgg actccattca aacaccgtat
    19141 catcttcagt aattacagga gcttg
//
LOCUS       DAOOYB010000021        18237 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_2947_length_18237_cov_9.266417, whole genome shotgun sequence.
ACCESSION   DAOOYB010000021 DAOOYB010000000
VERSION     DAOOYB010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 18237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18237
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2947_length_18237_cov_9.266417"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="PL056_02275"
     CDS             <1..726
                     /locus_tag="PL056_02275"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPM45874.1"
                     /translation="RFVLSCTDDLLKEVRKLSDHYGILIETHGSEHKEEVEYIRSKSG
                     FGNISYLYHIGALNERSVIAHTVHLDEIEVDLIRNLKVSTVHCPSTNLKLGSGIAPVY
                     RYLQEGIKVGIGADGAPCNNALNIFSELKVASLLQKGLAHNAKIMTAKDTLRLATINS
                     AKILGIEGLVGRLKEGMDADIVVLDMDTPQTLNFECHPSAAVVYGCDPRNVKATMVAG
                     KFLFRDGQFSQEINDLEKFFNEM"
     gene            807..1646
                     /locus_tag="PL056_02280"
     CDS             807..1646
                     /locus_tag="PL056_02280"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HPM45875.1"
                     /translation="MKKVVLMILAVLLMITGCCKKCEEKVDDVKPAEPKKMGKYAADI
                     EIISYRMGFDTAVTAFQSFPEMDTEAICKGVKDAAVKPVEPKYSDDVFNRSMARIRKD
                     KISVDAEKRLGEYFRNQPVSEKYMKEAAKKKGLKKLEKGVLLEVLKEGTGDKVTIKDI
                     AEVRYQGWDAKGTLFDSSFKRNRTSRFNLHDLIEGLRLAVLEMKKGGKYKVYIPEKAG
                     YGTKGFKNRIEAGMALLFEIEIVNVERGKAKDPETETQTENADPAQLMKLMETKNDGV
                     KKN"
     gene            1624..2112
                     /locus_tag="PL056_02285"
     CDS             1624..2112
                     /locus_tag="PL056_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HPM45876.1"
                     /translation="MTVSKRTDYISWDQYFMGVALLSAQRSKDPNTQVGACIVNSEHR
                     IVGVGYNGFPSGCDDSYLPWSREGSFLETKYPFVVHAELNAILNSIKNLRGCTIYVAL
                     FPCNECAKAIIQAGISEIVYLCDKYAETDVVKASKLMLEQAGVKMRKLDPVPEDIVLK
                     FS"
     gene            complement(2113..4242)
                     /locus_tag="PL056_02290"
     CDS             complement(2113..4242)
                     /locus_tag="PL056_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45877.1"
                     /translation="MKRFNIAGLITSVLFFLFFFVWGTKGKFWTVITAFYPAFHITFL
                     IVIISLYAIYATIPNTSASAFNFIVRHRFKFLPFAGFLFSALIAVFVFDSIPHVIDAA
                     HFLWTARVFIETGSFHLPASELYEYYQNTFNVEINNRFFSLFLPGFSVFLIPFELLGI
                     SQFFTPLCNGIAVFLLGKIADRQTDDPRISFFAMIFAVCSSFYLFMGASYMTHSFNLV
                     LTLLPIYLILSSDRPRTYVLSGISGAILLFIRPQNAVFLYVAILAFMYLKNRKLRDAV
                     YFTIPFIFTGFLLLFHNYFYTGHPTLFPQDVYFFTREPYPFCHRLGIGKGCPNTEGDF
                     LPAEGLTFSYAFWVAFTKITLMNFNLSGHPLIFIFVALSFVFAFRKSIELALFFIIFF
                     AGYYFFYLPGNLFGPRYLAETASLLLILAGIAFFKIFDAQKRWLKPLIASLPVATTLF
                     LSTTIMPALISQFSDAFWDTDRAVERAIEKEGIKNSIVFVPNLYGSVFLNLMEKPPYD
                     RHGNLILLDLGEENSYATAYFMEKHDFDGAYVVDYYSRIAVRTAVTPVFTQERTRIQF
                     EFEDKRLPRTGRPDYGVNFAASEDEYKKFYPVKQLTIDVSNGSVFAMRFEDIHEKSYY
                     DFSHPVLESGEYDIELYYVSDECGADFAFEIDGKQIGIFSSHSDTQTRNVFNTSFFLE
                     AGNHFFKLVPLRGRSCLMIDNMVMELN"
     gene            complement(4239..5882)
                     /gene="dnaX"
                     /locus_tag="PL056_02295"
     CDS             complement(4239..5882)
                     /gene="dnaX"
                     /locus_tag="PL056_02295"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HPM45878.1"
                     /translation="MSYQVVARKWRPQRFEQVVGQDHITETLKYAVLNKRIAPVYLFT
                     GIRGTGKTTLARILVKSVNCLDLQSDGEPCNKCENCIEIMEGRSPDFVEIDGASNNSV
                     DDVRNIKENISYYPVKSKFKVYIIDEVHMLSKSAFNALLKTLEEPPSHTIFILATTDH
                     QKIPATVLSRCQRYDLRRLSLDDVAKQLSKILDSEDHKYDMESLYAIAREGEGSMRDS
                     QTILEQLITFGDGSITEQECSLILGCSDKKQIRGIIEAVIKKDPATSVALVTEIYRNG
                     KSIEKAAKDILYLLHHIVLFSELKNHDYLESPKEEKEWIEKCSAMASTPDWIRLFRFW
                     NEQHESIKTSDFALMIFEVAVITACNFPTMDDFRTFAKALSEGASVINLDKNGNEPEK
                     EKKNLSETVSLKDPEPEKPVSFETKTPDTPDEPVREYNWEAFTEYLKDEDPYFYGALV
                     NIPYSEDDYLIALSVDMNLEMAKKDILHRLKLKFSEYFKGTRELEIRLETGSDLSIHN
                     EKEIERLNEIKSKKDSIINSSAVKEAIQLGLELKKVDVE"
     gene            complement(5966..7015)
                     /locus_tag="PL056_02300"
     CDS             complement(5966..7015)
                     /locus_tag="PL056_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004892634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HPM45879.1"
                     /translation="MISIKSGNILESKADAIVNTVNTKGVMGKGIALQFKEVFPMMFV
                     DYKKACKSGIVMIGKMHVWHNNAIFGPKYIINFPTKNDWKNPSKMEYIERGLVDLEKT
                     IFELGIGSIAIPPLGCGNGGLDWNIVKSKIINSLSKFNSLNIEIYEPSGAPEILSKAP
                     GKTIKMTFSRAIMLKLFKRYCILGYELTLLEVQKLCFFLQEFGEPLHLKYEKGEYGPY
                     ADQIRHVLNIFEGYYTQGFRDGSRNKPDTIINLLPNAIVESENFIKNSFDSHQSLNRL
                     KKVQEFIEGFETPFGMELLSTVFWVIKNNHLDINNKPSIINAVLSWNSRKAKIMKPVF
                     INKAIDRIEKFQLII"
     gene            complement(7030..7701)
                     /locus_tag="PL056_02305"
     CDS             complement(7030..7701)
                     /locus_tag="PL056_02305"
                     /inference="COORDINATES: protein motif:HMM:NF025840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4433 domain-containing protein"
                     /protein_id="HPM45880.1"
                     /translation="MVEFTGVKNIVPHNPNQIPLFHITHVDNLYAIIKSGGLWCDRER
                     IIQGFSSANIAHKPLKDRRMATKVPLYLPKTLGDFVPFYFTTRSPMLFSVHTGSVDDC
                     SYGQESIIYLITTTENLTTCQRNWCFTDGHAVEAISEFYNNLNDMDKVDWNLINDWSW
                     HNTLEDSDRKRRKQAEFLVEKSVPFSAFIEISVINEEMERKIHKILEMAKINLAVSVK
                     PKWYY"
     gene            complement(7732..9582)
                     /locus_tag="PL056_02310"
     CDS             complement(7732..9582)
                     /locus_tag="PL056_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45881.1"
                     /translation="MKLSKVLDIVNQMEKSSFLKLIDNFSNDLKSSNNEIREILEKNG
                     CPLKNIENENIVKLFNFSQDKFENLFEKTLNYNSFQFDIICDIISRDGNSIMHYSWFQ
                     KLYQNEVEKLKNNIDLFLPKLEEENNELNFERKRDYTIYKMCVETAYENDSLINRENK
                     ITDEEKSILNTLASCLELSSEETRMIFYSITHLKRLEIDDIINSLKEIGVAFFNKKTL
                     NIYFPDEIISIIRKKSGRELPNKYFKRILLVLTEPELNRICKKHGLAIESSKTDDKIK
                     NIIKQGLSIKRTLSNDIFKNETSKTDKKSRLQEIINDLELPIEKIGATVDERIEKIIE
                     HFSKIELDENIGISIDGYENLIKDLTEKHPFICESIKEAFELSQENICNIDLLMDYNI
                     KPLDILDLVDQDNLKQFAIDKHIKWKGNVRNNILSSYRDMNNLFIENYPLIGRRDFNG
                     LKEKGLTIKESELGSKYEEITKEIFKNLGYNVNETRRNDINSAKNKIDILLDLGENSV
                     ILIECKTIKEGDFSKYSIVSRQIKSYVDHCANNGLRVEWTILVSEGFTDEFIEYCEYD
                     RDLNLSLIDSEGLLKIMTAFKESKLKNFPTKLFQKSGKLNAEMIIKKGLC"
     gene            complement(9606..10691)
                     /locus_tag="PL056_02315"
     CDS             complement(9606..10691)
                     /locus_tag="PL056_02315"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HPM45882.1"
                     /translation="MYRHLILLILSAFILFSCGVEEDETQPDNNNTNDGNTVPDNDET
                     LPDNELNDESNESGEITDEFAESFCYSGGAVLYEGDQQYKMCPGDFKRVLKQICRNGK
                     WVDEGDCTLNYAVVPDGWFKMGCDIDKEGSCEEDNIPVHEPRLSEYAIDKFEVNTERF
                     EQCIAAGACNNNNADEPHYRTSTETYSCNIGNSERKNHPANCVTWYGAKAFCEWAEMR
                     LPTEAEWEKAARAGNVQIYPWGDSPVPDCDHAIIKTVSSGCGYGVTFPIGTTPEGISQ
                     LGLFDMGGNVSEYVSDWYQKDFYSDPSAEDRDVKGPDEPEYDKFKVCRGGSYLYDERR
                     VRTAFRSACKLDDPAIDFGFRCAADRK"
     gene            complement(10692..12488)
                     /locus_tag="PL056_02320"
     CDS             complement(10692..12488)
                     /locus_tag="PL056_02320"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM45883.1"
                     /translation="MTKKDMEMMSADRNLAFLKQNDVENFEFAKAVALSVAIFHIPLI
                     LLDLHRYIFNRDLFFKPGFFHISIIHAVYIVILFGIYFAGLIFKRRYKDMEMCPVLTK
                     IYWRVFLYVAISYALLTTPTCHLMHGNTTTFFITILGVASAVKMRPLEFAINFLTLAI
                     LAIFSVSFVAAGSAFTGYVLDISVATFISIFINIVIYSRALVSFNHRINFEEEHSQKI
                     IANEANKAKTAFLANMSHEIRTPLSGIMGMHSLLHETGLTEEQKEYLGYAEKSSETLL
                     GVINDILDLSVIDSGNVSIEKKPVNFGKLIRNITETVKGYAEKDGLEISVKIDPSIPE
                     IIMLDQLRMTQILNNLLSNAVKFTEIGEVTVTCKNYSGMLNIDVRDSGIGIPEEKMEI
                     VFEKFTQIDSSFRKKYKGAGLGLSITKKLVEMMGGSISVRSNIPEKGTTFSFNIPIEA
                     NGDNDIFDSFETVIETARCLKGLKILFAEDNPVNKELVNRYLEKEGCRITNVNNGLEA
                     FEIYKLERFDLLLLDIQMPVMDGVEAIRKIRDFETDKGYRTIAIALTAYAMKTDRDKF
                     LSAGMDGYVSKPFSKEHLLKEMCLKITRKQGQ"
     gene            complement(12772..13506)
                     /locus_tag="PL056_02325"
     CDS             complement(12772..13506)
                     /locus_tag="PL056_02325"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HPM45884.1"
                     /translation="MTDKKIRINRFIAQSGIASRRKAEELINEGRVTINGRKAILSDT
                     VDADNDVVEFDGKKIQSADKLEYYLLYKPAGILSTSSDDRGRKTVSDLLPEGSKAVPA
                     GRLDLDTTGVLILTTDGELHYRLTHPSFNIKKIYKATIAGEFTEEKAKKITAGIEIDG
                     IIMRAEKLVILNRRGETVTDITIVLNEGRKREIKELVRAVGCRVIQLERVAFANITCN
                     EMNKGEIRPLTDTEISSLYKLTGLKK"
     gene            complement(13499..16210)
                     /gene="secA"
                     /locus_tag="PL056_02330"
     CDS             complement(13499..16210)
                     /gene="secA"
                     /locus_tag="PL056_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HPM45885.1"
                     /translation="MFKYMIKKIFGTENDRYLKSILPLVSRINDLETSVKPLSDNELM
                     LKTLEFKQRINNGESLDSILPEAFAVVREASIRTLGMRHYDVQMIGGIVLHQGKIAEM
                     KTGEGKTLVATLALYLNALEGKSAHLVTVNDYLASRDAEWMGQIYRFLGMSVGTIMNG
                     IGTKERRIAYGSDITYGTNSEFGFDYLRDNMKYDINHYVQRELRYAIVDEVDSILIDE
                     ARTPLIISGPSDESTDLYVRVNAVVRTLSDEKHFTKDEKSKNAILTPEGIAKLESMLG
                     VANLYAPENLELVHHVQQALKAIFMFHRDVDYVVKEFDGLQKVMIVDEFTGRLMEGRR
                     YSDGLHQALEAKEGVKVERENQTLATITYQNFFRMYNKLAGMTGTADTEAREFAEIYN
                     LGVTVIPTNRPVIRADRPDQIYKNEIAKENAVIKEILEKNGSGQPVLVGTVSVEKSER
                     LSHLLTKRGIKHNVLNAKFHMKEADVVAQAGRKGTITIATNMAGRGTDIVLGGNPEKL
                     AYAEAEKTMETVDTTTPEFKEILSKYERICAEEKKEVLTAGGLHIVGTERHESRRIDN
                     QLRGRSGRQGDPGSSRFYLSLEDDLMRIFGSERLSSVMDRLGIEEDQPIEHKLISKSI
                     ENAQKKVEGHNFSIRKNVLEYDDVMNQQRKTIYAMRRDILTGGQKNKDVIYSMIEEIV
                     NFCVTTSVPEKALPSSWDYESIREYIVSVTSPENSGGLENALDPQNFAGMKNSEQAMS
                     ALAQKIYDFLIERYDIKMAQYSTELSNEMERHIVLEVLDTYWRRHLQNMDHLREGIGL
                     RGYGQKNPKLEYKREGFRMFTDMINSIYFESVKRLFAVQVVTQEAVEKFEQKEQKKEA
                     EIQRNTTTSIEKEPVKRNAPKVGRNDDCPCGSGQKFKRCCMNKGIYD"
     gene            complement(16256..16558)
                     /locus_tag="PL056_02335"
     CDS             complement(16256..16558)
                     /locus_tag="PL056_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45886.1"
                     /translation="MAIYICHNCKRSLDEELEDIRGKVGRQDVCPHCYADLHCCLNCR
                     FHDVNYTNECREDSRSFIRERDKSNFCSSFEFIKDEGEDGSEEIDAKTKLGDLFKF"
     gene            complement(16721..>18237)
                     /locus_tag="PL056_02340"
     CDS             complement(16721..>18237)
                     /locus_tag="PL056_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45887.1"
                     /translation="NDIDMIQPDKDSDDLSDDLSDEADEESEALAESEDTESDETDDE
                     VELADVDYQGAEECPELAKAKFPYYNDDLSIHFCRKCDKPTVKDPQCVENLWKDTAAA
                     LYSVSPESECEHGYPCYMPMIIPETLEEWIAYEEKIGHKFPYRPHECDMVLSPGSWAT
                     DSTAGAVKHFDISDGKVGLFLKNVALDYKKYQTYSKTMEYDPETQTYRALSPDLLETG
                     SYNNGCMLHIVNNQHIYSEANTFRSYLTYSCSDGTRKVVYPRNIRYVSYTPALSEKWA
                     IANIQEEDGMPDYTMYAKVGTWKWTKLMEGLSLFPQIVNDKVVFHDIDGMAYYCDLSK
                     TPKSVNECVKINREGETIRFAVINENDETETAYESRLNSEVTITKITVSKEGKKTYSV
                     IENTFSETDPYWVKYGYTPENYRDGFIFYTESYITNENGDWNGNHCFYNLAKKKRTCM
                     KKLADHPFKTNPNLGFGEWEGKWIVYQFLQGSTQAVRDLDCYCEKEGVCPFEQP"
BASE COUNT         5103 a         3651 c         3564 g         5919 t
ORIGIN      
        1 agatttgtcc tttcatgcac cgatgacctt ttaaaagagg tcaggaaatt aagtgatcat
       61 tacggtattc tcattgaaac gcacggttca gagcataagg aagaggttga atatatcagg
      121 tcaaagagcg gtttcggcaa tatttcatat ctttatcaca ttggtgcctt aaatgaaaga
      181 tctgtaatcg cacacactgt gcatcttgat gaaattgaag tcgatctgat cagaaatctc
      241 aaagtgagca cagtgcactg cccatcgacg aacttaaagc tggggagtgg aatagcacct
      301 gtttacagat atttacagga aggaataaag gttggaatag gagctgacgg agctccgtgc
      361 aacaacgctc tgaatatttt ttctgaactt aaagtggcaa gtcttttaca gaaaggtctt
      421 gctcacaatg caaagataat gacagcgaag gatactttgc ggcttgcgac gataaatagt
      481 gcgaagattc tcgggattga gggactggtt ggtagattga aagaggggat ggatgccgat
      541 attgttgttc tggacatgga tactccgcag actctgaact ttgaatgtca tccgtcagcg
      601 gctgttgttt acgggtgtga tccaagaaat gtgaaagcga caatggttgc agggaaattt
      661 cttttccggg atggtcaatt ttcgcaggaa atcaatgacc ttgagaaatt cttcaatgaa
      721 atgtgaataa tacggcaaat aattggcgaa atgaataaaa tctgataaat taccctgttt
      781 tttgttaatt aagaaccgga gttgatatga aaaaagtagt tttaatgatc ctggcagttc
      841 ttttaatgat caccggatgt tgtaaaaaat gtgaagaaaa agttgatgat gttaagccgg
      901 ctgaacctaa aaagatgggg aaatatgctg ctgatattga gataataagc tatcgtatgg
      961 ggtttgatac ggcagtaacg gcattccaga gtttccctga aatggatact gaggcaatat
     1021 gcaaaggagt taaagatgcc gctgtaaaac ccgttgaacc caagtacagc gatgatgttt
     1081 ttaaccggtc aatggcaaga ataagaaaag ataagatcag tgttgatgcg gaaaaacggc
     1141 ttggtgaata tttcaggaat caaccggtaa gtgaaaaata catgaaagaa gcggcaaaga
     1201 aaaaggggtt gaaaaaactt gagaaagggg tgcttcttga agttttgaaa gaaggaacgg
     1261 gcgataaagt caccataaag gatattgccg aagttagata tcagggatgg gatgcaaaag
     1321 ggactctttt tgacagttca ttcaaaagga acagaacctc aagattcaac ctgcacgatc
     1381 ttatcgaagg gcttagactt gcagtgctcg aaatgaaaaa agggggaaaa tataaagttt
     1441 atattcctga gaaggcgggt tacggaacaa aaggttttaa aaacaggata gaagcaggaa
     1501 tggcccttct ttttgaaata gagatcgtaa atgttgaaag aggaaaagca aaagacccgg
     1561 aaacagagac gcagacagag aatgctgatc ctgcacaact gatgaaactg atggagacaa
     1621 aaaatgacgg tgtcaaaaag aactgactat atctcgtggg accagtattt catgggagtg
     1681 gctctgttga gtgcacagag gtcaaaagat cctaatactc aagtcggtgc atgcatcgtt
     1741 aacagcgaac acagaatagt cggtgtgggt tataacggtt ttccatctgg ctgtgatgac
     1801 agttatctgc cgtggtcaag agaagggtct tttcttgaaa caaaatatcc ttttgtggtt
     1861 catgcagaac ttaatgcaat ccttaattca ataaagaatc tgaggggttg cacgatctat
     1921 gttgcactgt tcccgtgcaa tgaatgtgcc aaagcgataa ttcaggcagg aatctctgaa
     1981 atagtttatc tctgtgacaa atatgctgaa acggatgttg tcaaggcatc aaagctgatg
     2041 cttgaacagg cgggtgtcaa aatgagaaaa cttgatcccg tccctgaaga catcgtgctg
     2101 aagttcagct gatcagttaa gctccattac catattgtct atcataagac atgatctgcc
     2161 ccttaacggg acaagcttga aaaagtgatt ccctgcttca aggaaaaaag atgtattgaa
     2221 aacatttctt gtttgtgtgt ctgagtgact gctgaaaatt ccgatttgtt ttccgtcaat
     2281 ttcaaaagcg aaatctgcac cgcattcatc ggaaacatag taaagttcga tgtcatactc
     2341 accggattca agcactggat gagaaaaatc atagtaactt ttttcgtgaa tatcttcaaa
     2401 acgcattgca aaaacacttc cgttggaaac atctatcgtg agttgtttta caggatagaa
     2461 ttttttgtat tcatcttcgc ttgctgcaaa attaacaccg tagtcaggcc ttccagtcct
     2521 gggaagccgt ttgtcttcaa attcaaactg aatccttgtt ctttcctgtg tgaaaacagg
     2581 cgtaactgct gttcttactg caattctgct gtagtaatcg acaacatacg ccccatcaaa
     2641 atcatgtttt tccatgaaat atgcagttgc ataactgttc tcttcgccaa gatctagaag
     2701 tataaggttc ccgtgtctgt cataaggcgg tttttccata aggtttagaa aaacgctacc
     2761 gtacagattg ggaacgaaaa ctatactgtt ctttatccct tctttttcaa tggctctttc
     2821 aacggccctg tctgtgtccc agaaggcatc agagaactgg ctgatcagcg ccggcattat
     2881 agttgttgaa agaaacagtg tcgtggctac aggcaaagag gcgattagag gtttcaacca
     2941 tcttttctga gcatcaaata ttttgaaaaa tgcaatgcct gcaaggatga gaagcagtga
     3001 agcggtttca gcgagatatc tgggaccgaa aagattgcca ggaagataaa agaaatagta
     3061 gccggcgaaa aagattatga aaaaaagagc aagctcgata cttttcctga atgcgaaaac
     3121 aaatgaaagt gctacaaaaa taaaaataag cggatgtcct gaaagattaa agttcatcaa
     3181 agttattttt gtaaaagcga cccagaatgc atagctgaaa gtgagtccct cggcagggag
     3241 aaaatcacct tcagtgttcg ggcatccttt tccaatgccg agccggtggc agaatggata
     3301 aggttctctt gtaaagaaat aaacatcctg cgggaacaga gtggggtggc cggtgtaaaa
     3361 atagttgtgg aaaagaagca gaaaccctgt aaaaatgaaa gggatggtga aataaaccgc
     3421 atcacggagt tttctgttct tcagatacat gaacgcaaga attgcaacat aaagaaaaac
     3481 agcgttctga ggacggatga aaagaagtat agcccctgat attcctgaaa gaacataagt
     3541 ccgcggtctg tcagatgaaa gtatgagata tatcggaagg agagttaaaa ccagattgaa
     3601 cgagtgggtc atataggaag cacccatgaa aagatagaat gaactgcaaa cagcaaatat
     3661 cattgcaaaa aaggaaattc ttggatcatc tgtctgtctg tctgcaattt ttccaagaag
     3721 aaaaaccgct attccgttgc agagcggggt gaagaactgt gatatcccga gaagttcaaa
     3781 aggaatgaga aaaacagaaa accccggtag aaacagtgag aagaaacggt tgtttatctc
     3841 aacattgaag gtgttctgat aatattcata gagttcgctt gcagggagat gaaacgagcc
     3901 ggtttcaata aaaactctgg cagtccatag aaagtgagcg gcatcaatga catgaggaat
     3961 ggaatcaaat acaaaaacag caattagagc tgaaaacaga aaccctgcaa acggcaggaa
     4021 cttgaatctg tgacgcacaa taaagttgaa tgctgatgca gatgtgtttg gaattgttgc
     4081 ataaattgcg tatagagaaa taattacgat cagaaatgta atatgaaagg cgggatagaa
     4141 agcagtaatt accgtccaga attttccttt tgtcccccat acgaaaaaga aaaggaaaaa
     4201 aagtacggaa gtgatcaacc ctgcgatatt aaaccttttc attccacatc aactttctta
     4261 agttccagtc cgagttgtat cgcttctttt acagcgctgc tgtttatgat ggagtccttt
     4321 ttgctcttta tctcattaag ccgttcgatc tctttttcat tgtgtatgct caggtcgctg
     4381 ccagtttcga gccgtatttc aagctcacga gtccctttga aatattcact gaatttcagc
     4441 ttgagacgat gcaaaatgtc ttttttcgcc atttccagat tcatatcaac ggaaagtgca
     4501 atgagatagt catcctcgct gtaagggatg tttacaagag ccccgtaaaa gtaagggtct
     4561 tcatctttta aatactcagt gaatgcttcc cagttgtatt cgcgaaccgg ttcatcaggt
     4621 gtatcagggg tctttgtttc aaaggaaacc ggtttttccg gttccggatc tttcaaggat
     4681 acggtttcag agagattttt tttttccttt tccggttcat tgccgttttt gtcaaggtta
     4741 attacggacg caccttcaga aagtgctttg gcaaatgtcc tgaaatcatc cattgtcgga
     4801 aaattacaag ctgttattac tgccacttca aatatcatca gggcaaaatc agatgttttt
     4861 attgattcat gttgctcatt ccagaatctg aagagtctta tccagtcagg tgtcgaagcc
     4921 atcgctgagc atttttctat ccactctttc tcttctttgg gggattcaag ataatcatga
     4981 ttcttaagtt cgctgaaaag aacgatgtga tgaagaagat ataaaatatc ctttgccgct
     5041 ttttcaattg attttccatt ccggtatatc tctgtaacaa gtgccacaga ggttgccgga
     5101 tcttttttta taactgcttc aattatgccg cggatctgtt ttttatctga acatcccagt
     5161 atgagagagc actcctgttc tgtaatactg ccgtcaccga aagtgatcag ctgttcgaga
     5221 attgtctggc tgtcacgcat acttccttca ccctctctgg caattgcgta gagagattcc
     5281 atatcatatt tgtgatcttc agaatcgagt atcttggaaa gctgtttcgc cacatcatca
     5341 agcgaaagcc gtctcagatc gtaacgctga catctgctta gaacggtggc aggtattttc
     5401 tggtgatctg tagtcgcaag gatgaaaatg gtgtgggaag gcggctcttc gagtgttttg
     5461 agaagtgcat tgaatgccga ctttgaaagc atgtgaactt catcgattat ataaaccttg
     5521 aattttgatt taacaggata ataggaaata ttttccttta tgtttctcac atcatcgaca
     5581 gaattgttag aagcgccgtc gatctcaaca aaatcagggc ttctcccttc cattatttca
     5641 atgcagtttt cacatttgtt gcacggttca ccgtcacttt gcagatcaag acagttcaca
     5701 cttttaacaa gaatcctcgc gagtgtggtc ttgccggttc ctctgattcc ggtgaaaagg
     5761 taaacagggg cgatcctttt gttgagaaca gcatatttca gagtttctgt tatgtggtcc
     5821 tgaccgacaa cctgttcaaa gcgctgaggt ctccattttc ttgcaacaac ctgataactc
     5881 attattccac cttaataaaa aattaagcac acggcattat ataaaacagt tcaaagctgt
     5941 aaagaaaaag agaatacggt tttttttata taatcaattg aaatttttca attcgatcaa
     6001 tagctttatt tatgaataca ggtttcatga tttttgcttt tctcgaattc caagataaaa
     6061 ctgcgtttat tatggagggt ttattgttaa tatctaagtg gttgttttta attacccaga
     6121 aaacggtgga gagcaattcc atgccaaaag gagtttcgaa tccttcaata aattcttgaa
     6181 ctttcttgag acgattaaga gattgatgag aatcaaaaga gttttttata aaattctcag
     6241 attcaacaat agcattggga agcaaattta ttattgtatc tggtttgttt cttgacccat
     6301 ctctaaaacc ctgagtgtaa tatccttcaa aaatattcag aacatgacga atttgatcag
     6361 catatggacc atattcgcct ttttcgtatt taaggtgaag cggctctcca aattcctgca
     6421 aaaagaaaca aagcttctgt acttctagta atgtcagttc ataccctaga atacaatacc
     6481 tcttaaaaag ttttagcatt attgctcttg aaaaagtcat ttttatagtt ttccctgggg
     6541 ctttcgagag aatttccgga gctcctgaag gttcataaat ttctatattt aaactattaa
     6601 atttgctgag tgaatttata atcttagatt ttacaatatt ccaatccagt ccaccattac
     6661 cacaaccgag aggaggaatt gcgatcgagc caattccaag ctcaaatatt gttttttcta
     6721 aatcaacaag accccgttca atatattcca tttttgatgg gttcttccaa tcatttttgg
     6781 tgggaaaatt tattatatat tttggaccga aaatagcatt attatgccag acatgcattt
     6841 tcccgatcat tacgattcct gatttgcatg cttttttata gtcaacaaac atcattggaa
     6901 aaacttcttt gaactgcaat gcgattcctt tccccattac accttttgta ttaacagtat
     6961 taacaatagc atcagctttt gattctaaaa tatttccgct ttttattgaa atcaatacga
     7021 gttcctccgc tagtaatacc atttaggttt aaccgaaaca gccagattaa ttttagccat
     7081 ttctaatatt ttatgaattt ttctttccat ctcttcgttt atgacagata tttcaataaa
     7141 ggcagaaaac ggaacagatt tttcaacaag aaattcagct tgtttccttc ttttccgatc
     7201 actgtcttct aaagtattat gccaactcca atcattaatc agattccaat caactttgtc
     7261 catatcattt aaattgttat aaaactctga tatcgcttct accgcatgtc catctgtgaa
     7321 acaccagttt ctttgacatg ttgttagatt ttctgttgtt gtaataagat aaattatgga
     7381 ttcttgtcca taagaacagt catcgacaga tccagtatga acagaaaaaa gcatcggaga
     7441 acgggtggta aagtaaaatg gaacaaaatc tccgagggtt tttggcaagt ataatggaac
     7501 ctttgttgcc attcttctat ctttcagtgg tttgtgagca atatttgcgg aagaaaaccc
     7561 ctgaattatt ctttccctgt cgcaccataa tccacccgac ttaataattg cataaagatt
     7621 atccacatga gtaatatgaa aaagaggaat ctgattcgga ttatggggca cgatattttt
     7681 cactccagta aattcaacca tttcgttcaa caatataaag cggttctatt tttaacacaa
     7741 cccttttttt ataatcattt ctgcatttag ttttccactt ttttgaaata gctttgtcgg
     7801 aaaattttta agtttggatt ccttaaaagc agtcatgatt ttcagcaaac cttctgaatc
     7861 aattagtgaa agatttaagt ctcgatcgta ttcgcaatat tcaatgaatt catcggtgaa
     7921 tccttctgaa accaaaattg tccactcaac ccgcaaacca ttattggcac aatgatcaac
     7981 ataagacttt atttgtctgc taacaatgga atactttgaa aaatcacctt cttttatggt
     8041 tttgcattca attaaaatga cagaattctc tccgagatct aacaatatat ctattttgtt
     8101 tttcgcacta tttatatcat ttctacgagt ttcgtttaca ttgtatccaa gatttttaaa
     8161 tatctccttg gttatctctt catatttaga ccctaactcg ctttctttta tggtcagtcc
     8221 tttttctttt aatccattga aatctcgtcg tccaattaga ggatagttct caataaacaa
     8281 gttattcatg tctcggtaag aagaaagaat attgtttctc acatttcctt tccattttat
     8341 atgcttatct attgcaaact gtttaagatt atcttgatca acaagatcca atatgtccaa
     8401 aggtttgata ttataatcca tcaacagatc aatattgcag atgttttctt gagaaagctc
     8461 gaacgcttct tttattgatt cacaaataaa tggatgcttt tcagttaagt cttttatcaa
     8521 gttttcatat ccatctattg aaatcccaat attttcatcc agttcaattt tgctaaaatg
     8581 ttcaattatc ttttcaattc tttcatcaac agtggcccct attttttcta taggcagttc
     8641 gagatcatta ataatttcct gaagtctgga ttttttatct gttttagaag tttcattctt
     8701 gaaaatatcg tttgatagag ttctttttat acttaatcct tgttttataa tattttttat
     8761 cttgtcatct gttttagaag attcaatcgc aagtccgtgt tttttacaaa ttctatttaa
     8821 ttccggttca gttagaacga gtaaaattct tttgaaatat ttgttcggta attctcgacc
     8881 tgattttttc ctaataatgc taatgatttc atctggaaaa tatatattaa gtgttttctt
     8941 gttgaaaaaa gcaactccga tttcttttag tgagttgata atatcatcga tctccagtct
     9001 tttaaggtgt gttattgaat aaaaaatcat tcttgtttct tcagaagata attctaagca
     9061 agaggcaaga gtgtttaaaa tgcttttttc ttcatctgtt attttatttt ctctatttat
     9121 gagagaatca ttctcataag cggtttcaac gcacatttta taaatagtgt aatctctttt
     9181 tctctcaaaa ttaagttcat tgttttcttc ttccaatttc ggcaaaaaaa gatcaatatt
     9241 gttcttgagt ttttcaactt cattttggta caatttctga aaccaggagt aatgcattat
     9301 tgaatttccg tcacgagaaa ttatgtcaca aatgatgtcg aactgaaagg aattataatt
     9361 gagtgttttt tcgaataagt tttcaaattt atcctgagaa aaattaaata attttacgat
     9421 attctcattt tcaatgtttt taagagggca tccattcttt tccaaaattt ctctaatttc
     9481 gttattgctg gattttaaat cgttagaaaa attatcaata agttttaaaa aagaactctt
     9541 ttccatctga tttacaatgt ccaacacttt tgatagtttc atggaaatct cctaaaattt
     9601 aattgtcatt tcctgtcggc ggcacagcgg aagccgaagt ctattgcagg atcatcgagt
     9661 ttgcaggcgc ttctgaaggc ggttctgacc cttctttcat cgtagagata agagccgcct
     9721 ctgcaaacct tgaacttgtc atattccggc tcatccggcc ctttcacatc tctatcttct
     9781 gctgagggat ctgaatagaa atccttctga taccagtcac ttacatattc tgaaacattt
     9841 ccgcccatat caaaaagtcc aagttgtgaa ataccttcag gggttgttcc gatggggaag
     9901 gtgactccgt atccgcaacc ggatgaaact gtttttataa tggcatgatc acagtcggga
     9961 actggcgaat ctccccacgg atatatctgg acatttccgg ctcttgccgc tttttcccat
    10021 tccgcttcag tcggcagacg catttcggcc cattcgcaga aagcttttgc tccgtaccat
    10081 gtcacacagt ttgcgggatg attctttctt tcgctgttgc cgatgttgca ggaataagtt
    10141 tccgttgaag tcctgtagtg cggttcatca gcattgttgt tgttgcaagc acctgccgct
    10201 atgcactgct cgaacctttc cgtatttact tcaaacttat cgatagcata ttcagaaagc
    10261 cgcggttcat gaacaggaat gttgtcttct tcgcaggatc cttccttatc aatgtcacag
    10321 cccattttga accatccatc cggaacaacg gcataattca gtgtgcaatc accttcgtcg
    10381 acccattttc cattcctgca gatctgtttc aatacccgtt tgaaatcacc gggacacatt
    10441 ttgtactgct ggtccccttc ataaagcaca gctcctcccg agtagcagaa cgattcagca
    10501 aattcgtcag tgatctctcc tgactcattc gactcatcat tcagttcatt gtccggcaaa
    10561 gtttcatcgt tatcgggaac agtgtttcca tcatttgtat tattgttgtc aggttgtgtt
    10621 tcgtcttcct ccactccgca cgagaacaga atgaacgcag agaggatgag taaaataaga
    10681 tgtctgtaca tttattgccc ctgttttctt gtaattttta aacacatctc ttttaacaag
    10741 tgttcttttg aaaaaggttt cgaaacatat ccgtccattc cggcagacag aaatttgtcc
    10801 ctgtccgttt tcattgcata ggcggtaaga gctatcgcaa ttgttctgta acctttatcg
    10861 gtttcaaaat ctcttatttt ccttatggct tcgacaccgt ccatcacagg catctgaatg
    10921 tcaagcagaa gaagatcaaa tctttcgagc ttatatattt caaaagcttc aagaccgtta
    10981 ttgacatttg ttattctgca cccttccttt tcaaggtatc tgttgaccag ttccttgttg
    11041 accgggttgt cttctgcaaa aagtattttt aatcctttaa ggcagcgggc tgtttctatt
    11101 acagtttcga aagagtcaaa aatatcatta tctccgtttg cttctatcgg tatattgaaa
    11161 gaaaatgtag ttcctttttc aggtatgttg gaccgtacgc tgatactgcc gcccatcatt
    11221 tcaacaagtt tttttgttat tgagagcccc agccccgctc ccttgtattt ttttctgaaa
    11281 cttgagtcta tctgagtgaa tttttcaaag acaatttcca tcttttcttc cggtattcct
    11341 atgccgctgt cccttacatc aatattcagc attccgctgt aattcttgca ggtgacggtt
    11401 acttctccga tttcagtgaa ttttactgca tttgaaagaa gattgttgag tatctgcgtc
    11461 attctgagtt gatcgagcat tattatttca ggtattgacg gatctatttt aactgatatc
    11521 tcaaggccat ccttttctgc ataacctttt acagtttctg ttatgtttct gatcagtttg
    11581 ccgaagttga caggtttttt ctcaattgaa acattgcctg aatcaataac gcttaaatca
    11641 agaatatcgt taattacccc cagaagtgtt tcactgcttt tttcggcata gcccagatac
    11701 tctttctgtt cttcagtaag cccggtttcg tgaaggagcg aatgcattcc cattattccg
    11761 cttaaaggtg ttctgatctc gtggctcata tttgcaagaa atgcagtctt tgccttattt
    11821 gcttcgtttg caattatctt ttgggaatgc tcctcttcaa aatttattct gtggttgaac
    11881 gaaacaagag ctctgctgta aatgacaatg ttgatgaaaa tggaaatgaa tgtggcaact
    11941 gaaatgtcaa ggacatagcc ggtgaatgcc gagccggctg ctacaaaact gaccgaaaat
    12001 atagcgagga tggcaagtgt taaaaaattg atggcaaatt caaggggacg cattttgaca
    12061 gcgcttgcaa caccgaggat cgttatgaaa aaggttgtag tgttcccgtg cataagatga
    12121 caggtcggag ttgtaagaag cgcatagctg attgcaacat aaagaaaaac gcgccagtat
    12181 atttttgtaa gcacaggaca catttccatg tctttatatc gccttttaaa gatcagacct
    12241 gcaaaataaa tcccgaaaag tatgacaatg tacactgcat gaatgataga gatgtgaaaa
    12301 aatcccggtt tgaaaaaaag gtcacgatta aaaatataac ggtgaagatc aagcaggata
    12361 agagggatgt ggaatattgc taccgaaagg gcaaccgctt ttgcgaattc aaaattttca
    12421 acatcattct gttttaaaaa ggcaagattt ctgtcagcac tcatcatttc catgtccttt
    12481 tttgtcatgc ctgtcacctt ttattgttca atatgtcaaa atcccaattc tggaggtatc
    12541 atacctgaaa aggatttttt tgaaaattat tttgcattat catattgact caaagatgtg
    12601 cagcaggtag aattgatatg cctgcctggt gcgagacttc gggcgataga agttttttcc
    12661 ttgataagtt tttggggttt gtactaatac tgcttattaa agcggcgcgg ctttccccac
    12721 ctgtgagaac aggataaaac ttgcctgaag ttacggccga ggcgggtttt tttatttttt
    12781 aagtccggtg agtttgtaca gagagctgat ctcggtatcg gtaaggggtc ttatttcacc
    12841 cttattcatt tcgttacagg taatattggc aaaagcgacc ctttcaagct gaatgactct
    12901 gcatccgaca gccctgacaa gttcttttat ctctcttttt ctcccttcgt tcaggactat
    12961 tgtgatgtcg gtcacagttt cacctctgcg gttcaggatg acaagctttt cagctctcat
    13021 gataataccg tcaatttcga tgccggctgt tatcttcttc gccttttctt cagtaaattc
    13081 gccggcgatc gttgctttat atattttctt aatattgaat gaagggtggg tgagacggta
    13141 atggagttcc ccgtcagttg taagtatcag aacacctgtt gtgtcaaggt cgagccgtcc
    13201 ggcaggaact gcttttgaac cttcgggaag aagatctgaa acggtttttc ttccgcggtc
    13261 gtcagatgat gtcgaaagga tgccggcagg tttgtaaagc agataatatt caagcttatc
    13321 cgcactctga atctttttgc cgtcaaattc gacaacatcg ttatcagcat cgacagtgtc
    13381 tgacagaata gctttccttc cgtttatcgt gacacgccct tcgttgatca actcttcagc
    13441 ttttcttctt gaagctatgc ctgattgtgc gataaagcgg ttaattctga tctttttatc
    13501 agtcatagat ccccttgttc atgcagcatc gtttaaattt ctgaccgctt ccgcatgggc
    13561 agtcatcgtt ccttcccact ttcggagcat ttcttttcac aggttccttt tcaatggaag
    13621 tggttgtatt tctttggatc tccgcttcct ttttctgttc cttctgctca aatttctcaa
    13681 cggcctcctg agtaaccacc tgtacggcaa aaagtctctt gacagattca aaatagatgc
    13741 tgtttatcat atcagtgaac attctgaacc cttcgcgttt gtattcgagt ttggggtttt
    13801 tctgtccata gcccctaagc cctatccctt cacggagatg gtccatgttc tgaaggtgtc
    13861 ttctccagta agtatcaaga acttcaagaa caatatgtct ttccatttca ttgctgagtt
    13921 cagttgaata ctgagccatt ttaatatcgt atctttcaat gagaaaatca taaattttct
    13981 gtgccagagc agacatcgcc tgttcagagt ttttcatccc tgcaaagttc tgcggatcaa
    14041 gagcgttttc gagtccgccg ctgttttccg gggaagtcac tgaaacgata tattccctta
    14101 tgctttcata atcccatgaa gagggtagtg ctttttcggg aacggatgta gtgacgcaga
    14161 aatttactat ctcctctatc attgaataga tgacatcctt gtttttctgt ccgcctgtca
    14221 ggatatctct tctcattgca tagatggttt ttctctgctg gttcatgaca tcatcatatt
    14281 cgaggacatt ctttcttatg ctgaagttgt gtccctcaac ttttttctgt gcgttttcaa
    14341 tggatttcga tataagtttg tgttcgatcg gctggtcttc ctctatcccg agacggtcca
    14401 tgacggaaga gagccgttca cttccgaaaa ttctcatgag gtcatcttca agacttaagt
    14461 agaaccttga tgatccgggg tctccctgac gaccgcttcg gcctctgagc tgattatcga
    14521 tacgacgcga ttcgtgcctt tcggtgccta cgatgtgaag ccctccggcg gtaagaacct
    14581 cttttttctc ttcagcgcat atcctctcat atttactcag tatttcttta aattcaggag
    14641 tggtcgtatc aacagtctcc attgtttttt ccgcttcggc ataggcaagt ttttcagggt
    14701 ttcctccaag cacgatatcg gtgcctcgac cggccatgtt agttgcaatg gttattgtcc
    14761 cttttcttcc tgcctgtgcg acaacatcag cttccttcat gtgaaacttt gcattcagga
    14821 cattgtgctt tattcctctt ttggtaagaa gatggctcag ccgttccgat ttttcaacac
    14881 tgaccgttcc caccagtacc ggctgtccgc tgccgttttt ctcaaggatc tcttttataa
    14941 cggcgttttc ctttgcgatc tcatttttgt atatctgatc ggggcggtcc gctcttatga
    15001 caggtctgtt ggtcggaatt acagttactc cgaggttgta tatctcggca aattcgcgtg
    15061 cttcagtgtc tgccgttcct gtcatgccgg cgagtttgtt gtacattctg aaaaagttct
    15121 gatatgtgat ggttgcaagt gtctggtttt ccctttcgac ctttacccct tccttcgctt
    15181 caagtgcctg atgaagtccg tcgctgtatc ttctcccttc catcaacctt ccggtgaatt
    15241 catcaacgat cattactttc tggagtccgt caaattcttt tacgacataa tcaacatccc
    15301 tgtggaacat gaaaattgcc ttcagtgcct gctgaacatg gtggacaagt tcaaggttct
    15361 caggtgcata aagattcgca actccaagca ttgattcaag ttttgcaata ccttcaggag
    15421 taagtatggc gtttttcgat ttttcatctt ttgtaaaatg tttttcatcg ctcagtgttc
    15481 tgacaacagc gttcaccctc acataaagat cggtgctttc atcactcgga cctgagatga
    15541 tgagaggagt tctcgcttca tcaataagga tcgagtcaac ttcgtcaact atggcatatc
    15601 tgagttctct ctgaacatag tgattgatat cgtatttcat attgtcgcgg agataatcaa
    15661 agccgaattc gctgttagtt ccgtaagtta tatccgatcc gtatgcgatg cggcgttctt
    15721 ttgtccctat tccgttcatt attgttccga cgctcatgcc gagaaaacgg tagatctgcc
    15781 ccatccactc ggcgtctctt gatgcaagat aatcgttgac tgtgacgagg tgtgcacttt
    15841 tgccttcaag tgcgtttaaa tagagagcaa gtgtcgcgac aagcgtttta ccttcacctg
    15901 ttttcatttc agctattttt ccctgatgaa ggacaatgcc gcctatcatc tggacatcgt
    15961 aatgtctcat tccgagagtt ctgatggagg cctctcttac aacggcgaaa gcttccggaa
    16021 gtattgaatc gagcgactcg ccattattga tgcgctgctt gaattcaagg gttttgagca
    16081 tcagttcatt gtcacttaag ggcttgactg aagtttcaag atcgttaatt cttgaaacaa
    16141 gcggcaatat cgatttaaga tagcggtcgt tctcagttcc gaaaatcttt tttatcatgt
    16201 atttgaacat cggggcactc caaaatcaaa aaataaagaa aaacaggtga tatgatcaga
    16261 atttgaacag atcgccaagc tttgtttttg catcgatctc ttcgctcccg tcctcacctt
    16321 cgtcttttat gaactcaaat gatgaacaga agttcgattt atccctttct ctgatgaaag
    16381 accgtgaatc ttcgcggcat tcatttgtgt agttgacatc gtggaaccgg cagttgagac
    16441 agcagtgaag atcagcgtag cagtgcggac agacatcctg ccttccgact ttgcctctga
    16501 tatcctcaag ttcctcatca agtgatcttt tgcagttatg acagatgtaa atcgccattt
    16561 gttcctccat taaaaaacat aacggaatac aatataatta gagtagctga aaaatcaaga
    16621 agcagtttta ctgaagttac ccaaaaaatt ttcagtggat tttacctaat agtgtgagcc
    16681 gttttgattt gaggagaaat cattccctca ttcgacctgc tcatggttgc tcaaacgggc
    16741 acactccttc tttttcacaa tagcagtcaa gatctcgaac tgcctgtgtt gatccttgaa
    16801 gaaactgata aactatccat tttccttccc attctccaaa tccgagattg gggtttgttt
    16861 taaatggatg atcggcaagt tttttcatac atgttctttt cttctttgcc agattgtaaa
    16921 agcagtgatt cccgttccaa tctccgtttt cattggtgat ataagattct gtataaaata
    16981 taaatccgtc tctgtaattt tccggcgtat aaccgtattt tacccagtaa ggatcagttt
    17041 ccgagaaagt gttttcaatc actgaatatg ttttcttccc ttcttttgaa acagttattt
    17101 tcgttattgt gacttcagaa tttaatcttg attcatatgc tgtctctgtt tcgtcattct
    17161 cgtttattac agcaaatctt attgtttcgc cttcacggtt tattttaaca cattcattga
    17221 cacttttggg agtttttgac agatcacaat aatatgccat tccgtcaata tcatgaaaaa
    17281 caactttgtc attcacaatc tgcggaaaaa gagacaatcc ttccatcagt ttcgtccatt
    17341 tccatgttcc gactttcgca tacattgtgt agtcaggcat tccatcttct tcctgaatat
    17401 tagctatcgc ccatttctca cttaacgctg gagtgtagga gacatatctt atatttcgag
    17461 gataaaccac ttttcttgtt ccgtcactgc atgaataggt taaataactt ctgaaagtat
    17521 tcgcttcaga ataaatgtgc tggttgttta ctatatgcaa catgcatcca ttgttgtatg
    17581 aaccggtttc gagcagatca ggagacagtg ctctgtatgt ctgtgtttca ggatcgtatt
    17641 ccattgtctt ggaataagtc tggtattttt tgtaatcaag agcaacattc tttaaaaaaa
    17701 gccctacctt cccgtcactt atgtcaaagt gtttaactgc tcccgctgtg gagtctgtcg
    17761 cccaacttcc cggcgaaaga accatatcac attcatgtgg gcggtaagga aacttgtgcc
    17821 caattttttc ttcatatgca atccattctt cgagtgtttc aggaattatc attggcatat
    17881 aacacggata gccatgttca cattcagatt caggggaaac agaataaagt gccgccgccg
    17941 tatccttcca caggttttca acgcattgcg gatctttcac tgtcggttta tcacatttac
    18001 ggcagaaatg aatgctcaag tcatcattgt aatagggaaa ctttgccttt gcaagttcag
    18061 gacattcttc cgctccctga taatcaacat ctgcaagctc aacttcatcg tctgtttcat
    18121 ccgactccgt gtcttccgac tccgccaagg cttccgactc ctcatcagct tcgtcggata
    18181 aatcgtcgga caagtcgtcg gaatctttgt caggttgaat catatcaatg tcgtttt
//
LOCUS       DAOOYB010000022        18058 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_2992_length_18058_cov_12.377270, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000022 DAOOYB010000000
VERSION     DAOOYB010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 18058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18058
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2992_length_18058_cov_12.377270"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            161..>2949
                     /locus_tag="PL056_02345"
     CDS             161..>2949
                     /locus_tag="PL056_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019490986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM45888.1"
                     /translation="MVNILKIKSLLDSFDFTSIFVNELGWSHPKNIKSLSIQINGMEF
                     SKKMIAELAGVAVFEISSSDGSIPDARTRASIYKEIVPLHHENILIFVDKRRAMSLWY
                     WVKRIEGKLFPRDHIFVKGQPGDLFLSKIKSMVFDISDFDDFGNVSVLQVTSKLKDAL
                     DVEKVTKRFFNEFQEEHVRFIEYIEGIDDEKDRKWYASILLNRLMFIYFLQRKLFLDN
                     GNEKYLQNKLEYIKSFGKDLYYEKFLKILFFEGFAKPEDKRNSESNKLLGNIKYLNGG
                     LFLEHRIETSWRKIKIKDKAFENIFSLFERYSWNLNDTPGGKDNEINPDVLGYIFEKY
                     INQKAFGAYYTCPEITEYLCERTINRLVLEKAERAGKKFDSIGEMLIHADEELTKELL
                     LDFLPKLSLLDPACGSGAFLVAAMKTLINLYSALIGKIEFLQDQSLKLWIKKARLEHP
                     SIAYFIKKKIITDNLYGVDIMEEAVEIAKLRLFLALVASASKAEELEPLPNIDFNILS
                     GNSLIGLMKVDDKDFENKTANIFRMRSYKEILDEKNKLIDLYRHASMYAEDLKSMRDS
                     IENEKKESSLNLNEILHDMFWKELKIKFEEATWDDKKNSEGKTKKRNLEIKDIEALKP
                     FHWGYEFDRIINKKGGFDAIITNPPWEILKPNAKEFFEEHSELVTKKKMTIKEFEKEQ
                     KKLLKDPEIRKLWLEYLSQYPHVSEYFRTSPQYKNQISIVNGKKAGTDINLYKLFTEQ
                     CFNLLRDGGCCGIVIPSGIYTDLGTKQLREMLFNETAVTGLFCFENRKGIFENVDSRF
                     KFVVLSFEKGGKTADFPAAFMRHHVKELERFPREGAINISVDLVKKLSPDSLSVMEFK
                     NDMDIVIAKKMMKFPLLGEKIEGTWNLKLGNEFHMTNDSYLFKTQPGKGRLPLYEGKM
                     IHQFVNNYAEPKYWI"
     assembly_gap    2950..3049
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3226..3954
                     /locus_tag="PL056_02350"
     CDS             3226..3954
                     /locus_tag="PL056_02350"
                     /inference="COORDINATES: protein motif:HMM:TIGR04255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04255 family protein"
                     /protein_id="HPM45889.1"
                     /translation="MKKYNNPPIKEAIFDIRFNERISEGDIQKISETFFKEVEKEFPD
                     KKLQRSFEENITINPDGERKIESKNEITGYLFWSGDKKEVVQTRIDGFAYNILEKYEN
                     WEFFSKKAMNLFNVLKKVDTERTISRIALKYINVINLPLDNLKISDYISNMPPMCDEF
                     PQTMKGFILKTHFVENDVNIINIEKILPLKDNILPFVIDIDVFVQNPSFEKADLQKRM
                     EELRKTKNRIFELCITEKTRSLFR"
     gene            3951..4352
                     /locus_tag="PL056_02355"
     CDS             3951..4352
                     /locus_tag="PL056_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45890.1"
                     /translation="MKYDITIDGSSVQTKSPVSDFALKYIEDVSRKETLKHFYILENE
                     FETLKEKWKKEVKLTSSMTEIINNENYQKIIELGYDVIDFIYEELKKEPDFWFYALKE
                     ITGADPVRSSNYGNLKAMTQDWISWFKSNGF"
     gene            4354..4800
                     /locus_tag="PL056_02360"
     CDS             4354..4800
                     /locus_tag="PL056_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45891.1"
                     /translation="MKQEELSFLFPNSQKEPFTETSPATNSYNCIAWAANDNSKWYWP
                     ENHCYWPEGVEKKETIDSFIKMFSTFGYEICSDGLCEEGIEKIVIFSDENGKPTHAAR
                     QIDENFWTSKLGREIDVSHTLNAMCNGDYGNPAVFMKRRKKQSKEY"
     gene            4801..8097
                     /locus_tag="PL056_02365"
     CDS             4801..8097
                     /locus_tag="PL056_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019490988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HPM45892.1"
                     /translation="MPRIFDNIEEKLLSALEQSLPLAHRSDFCVGYFNLRGWKKISSF
                     IDNFKGGENNCCRLLVGMQRLPEEELKLALYEEDKSLNLDRPTAIKLKKKLAEDFRTQ
                     LTYGFPTNDDEVGLRKLSVQLKSEKLHVKLYLSHQLHAKLYLAFRNDPINPIIGYLGS
                     SNLTFSGLSEQGELNVDVLDNDAAKKLSNWFNDRWNDQWCIDISKELIEIIEESWARE
                     KPVPPYHIYIKIAYHLAQEARAGLSEFRIPKEFGNKLFDYQAAAVKIAAHHINKRNGV
                     IIGDVVGLGKTLMATAVAKVMQEDRFSDTLIICPKNLVQMWEDYVANYRLIAKVISIT
                     KVNDNFHNEMIRYSLVLIDESHNLRNRFGKRYNAIKDYISKNNCKCMLLSATPYNKTF
                     LDLSSQLALFLPDDEDIGIRPEKLIKEIGETEFIKRHQCPLRSLAAFEKSDFAEDWRE
                     LMRLYLVRRTRSFIQNNYAEIDSETGRKHLTFEDGTKSFFPIRVPKTVTFPSKNNQYS
                     ILFSDTVVKTINELKLPRYGLGNYVAPTHEKEPSQNDSKLLSDLSRAGKRLMGFCRTN
                     LFKRLESSGFAFIQSIERHILRNYVFLYAIENNLSLPVGTQNADFLDPEINDSEEKEN
                     IIKLKTVDDFKNQAKPIYDKYLKHQKNYFKWIKSEYFIENLKKDLIYDAEKLIKVLEI
                     CGGWKECEDPKLEELKKLINKTHKNDKVLIFTQFADTVEYIEKALKNKDDSDILGVTG
                     DSDDPTMCAWRFSPVSNKKENVIKKDQEVRILISTDVLSEGQNLQDANIIVNFDLPWA
                     IIRLIQRAGRIDRIGQRSDKIICYSFLPADGVENIIHLRRRVRERLNQNAEVVGTDEA
                     FFEDENTKRKIVDLYHEKAGVLDDDENEVDLTSYAYEIWKKAIDSDPDLKKIIPEIPN
                     VVYSTKQSDKNEGVIVYVKTQEGTDALALIDKNGESITESQYEILKVAQCEPLTQAVE
                     KFENHHELVKKGVKIIAEEERSIGGQLGRSTGTRFKIYEKLKNYAQKYKDTVLDIPSL
                     HKVVDEIYNNPLKQSAIEIINRQIKASISDQNLVDLVISLREEDKLCAIQGEKERKET
                     QIICSMGIRE"
     gene            8216..8515
                     /gene="rpsT"
                     /locus_tag="PL056_02370"
     CDS             8216..8515
                     /gene="rpsT"
                     /locus_tag="PL056_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008401332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HPM45893.1"
                     /translation="MAHHKSAKKRIKTNEKKRLYNRSIKSKVKTFTRYAVETIEAAEK
                     NTAGAAEMKTDAAAKLTKAVSEIQKAVSKGVLQKNTAGRKVGRLTIRFNKVFAAQ"
     gene            8517..9773
                     /gene="hutI"
                     /locus_tag="PL056_02375"
     CDS             8517..9773
                     /gene="hutI"
                     /locus_tag="PL056_02375"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HPM45894.1"
                     /translation="MRYSLVIENISSIFTAENSKAVLTGGDLAIASDMSGKICFAGKK
                     SELLKIDSSYRDNSESVVDAGNRTVFPGLVDSHTHLVFAATREDEFEMKARGATYEEI
                     ASAGGGILNSAKKTASATLDEIVFQSAKRLEQVISYGVTTIEIKSGYGLDLESELKLL
                     KAASELKKLYPVEIVPTFLGAHAYPEKFKNDHDGYIDLIINEMLPEVKNQKLAEFVDV
                     FCEEGYFSLKETQKILRAASDLGFGLKLHADEFNSLGGTELAAEMGAASVDHLEEITE
                     EGIRKLADSGTVAGVLPVTSVFSRLPFAPAQKMIDNGVTVALATDMNPGSCMCGFLPL
                     AASIGATQLRMSVEDSIRGITVNGARSLKRESAKGSISVGKDADLVIMDTSSWIYPVY
                     HFGHNHVHKVFVKGRALDLFKEKNLI"
     gene            9802..10770
                     /locus_tag="PL056_02380"
     CDS             9802..10770
                     /locus_tag="PL056_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45895.1"
                     /translation="MRSIPIFILFAVLTASCSSDTKPVESKWRVLDEQIYAPRIDEET
                     VFTDLDELEKLMFAPTGFFKEKSGMHSFEQKMAYLTNSVVNEEKRELTLEETVKFSSD
                     KDGNFKMRYRNNRHEGWSMVWKDNFLYRRQLGGEYTRTVSMGEHVYLKESLFSSIPSV
                     YAMLRNNARISSHDRKKINGSMATAVIISFGDKKIKREELPEKRYLQNLQGTEEMKND
                     QLIYDLAEKEKKNITGEMTVFVDDNYSVVRMEIKSSFELKAENVFFSIEGERTLSKKV
                     AEKVEIPKYNEEYHRRTLDASVNIMKDRKNDKERDEQTEESDQHSE"
     gene            10718..11077
                     /gene="rsfS"
                     /locus_tag="PL056_02385"
     CDS             10718..11077
                     /gene="rsfS"
                     /locus_tag="PL056_02385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HPM45896.1"
                     /translation="MTKNEMSKLKKVISTLSDKKGINITAFDVDRQSGYTDYMVFVTG
                     TSIQHNNTMSEALMRELKTAGFNKPLVEGSSTAKWILLDAGDVVVNIMLDELRDYYAL
                     EDIWVDSSKVDISSYLS"
     gene            11077..11514
                     /locus_tag="PL056_02390"
     CDS             11077..11514
                     /locus_tag="PL056_02390"
                     /inference="COORDINATES: protein motif:HMM:NF014629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HPM45897.1"
                     /translation="MKITLIVVGKLKKGAGFTELDDFYKSRIKPYSQLEIIELKEKND
                     IRLETESILKSIPSGSFTVALREEGVKMDSLEFSKLLNSRNESHGHITFIIGGAYGFR
                     NVNENISLSIAPWTLPHQLARIVLLEQIYRGLSIIKGSGYHHG"
     gene            11584..12456
                     /locus_tag="PL056_02395"
     CDS             11584..12456
                     /locus_tag="PL056_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45898.1"
                     /translation="MMGIKKLFAVIFVSAVFTAQVYAAQRMDFMLFNLSASNDPQKDF
                     LEMSKEFTAVMAPMFFGEAETRGSSGFEIGLGYSLTKINLNADYWKFSLNDEPVDSGV
                     PPFYNGIDLHVSKGFGFGLKLYGNMRYYVLTEMLSGGGGFEYAINEGLKMAPDISIGG
                     GYNRLFGATDFNMQMIELRLKISKTFVAGYQIKLIPFIAYSHLFSWAKSGRLGGYFPQ
                     GTSDPSQVSSSNPFIINEDGEPFYFKEEFINVDRIFIGFKMVSGYFGFELEAAIPVNA
                     GKTFSFNAGISASM"
     gene            12459..13124
                     /locus_tag="PL056_02400"
     CDS             12459..13124
                     /locus_tag="PL056_02400"
                     /inference="COORDINATES: protein motif:HMM:NF039343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45899.1"
                     /translation="MIDLHSHLLSGLDDGPESVDDSLEIIKYLSSFGFTELVPTPHKF
                     HALFNPVASEVIEKIENLKRSMVKRFSFEYNCNLQSIKDLEDHHEICRTSDDRKVILV
                     EFASGVVRRSEIENSVQSLNFAGFVPLMAHIERYFKKDEFWIELKKKYSVLFQGSIRT
                     FAKPNFNIKKKQLIRLMELDLIDNLATDIHKVSQLVKIEKGLDFIHKAFRNYEKDLFT
                     DKF"
     gene            13197..14432
                     /locus_tag="PL056_02405"
     CDS             13197..14432
                     /locus_tag="PL056_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011877640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA modification/repair radical SAM
                     protein"
                     /protein_id="HPM45900.1"
                     /translation="MDVMKKLEILSDSAKYDASCASSGSNRATPKGGIGNGKQCGICH
                     SWADDGRCVSLFKVLFTNYCIYDCAYCINRRSNDIFRTAFTPKEIADITIEFYKRNYI
                     EGLFLSSGVIKSPDHTMELLTEAIRILREEYLFGGYIHVKAIPGASKELIEKAGKIAD
                     RISVNIELPTKKSLELLTDKKHTDIFTPMKLITEGVSTSIAERKVSRKAPKFAPAGQS
                     TQMIIGATPETDLTIVKLSEAMYNKFDLRRVYYSAFIPVNSDLRLPVLREPPLLREHR
                     LYQADFLMRQYEFSADEILSPEHPMLDIALDPKANWAMNNFHIFPVEVNRADYQLLLR
                     VPGIGPRSAKRIVVARRSHNLSFENLRSLGVVLKRARFFITCKGKRMPFISEKFATVY
                     KSLSENHEPGPFQLPLFNT"
     gene            14437..15195
                     /locus_tag="PL056_02410"
     CDS             14437..15195
                     /locus_tag="PL056_02410"
                     /inference="COORDINATES: protein motif:HMM:TIGR03915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03915 family putative DNA repair protein"
                     /protein_id="HPM45901.1"
                     /translation="MNTLYLFDGTLETFISIIPVLWAISEPSDRLLSQSEYTPGIFEK
                     TVYLDERSSADLPVKNNSIPKEVINAVVYAFSSGIVEAVEKTPQYLNYIKNNGIDAMS
                     HLAEPSVSVIFKAARKTSSEAHKLKGILRFRKLGNILYGPYFSKCFVLPILAEHFKHR
                     LPNESWILHDTERKTGVFYNGKTVHQAFFSPRELFEITNEYEKSGVEDQFETLWKKYF
                     DTIAIEYRKNPKLQQSFMPKQYWRFIPEVAKRVL"
     gene            15257..17266
                     /locus_tag="PL056_02415"
     CDS             15257..17266
                     /locus_tag="PL056_02415"
                     /inference="COORDINATES: protein motif:HMM:NF012632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45902.1"
                     /translation="MKNKLIFIVLFLVLLSFLSCGNTKTGSENNNENPDSEIINESEW
                     SVLAAGGHHTCGIKNGELFCWGANYIGQFGNGTQTDNTTPLKIGIENDWTDVSAGYEH
                     TCAIRNGELYCWGNLYFAYLIPGVDEEMLSPGKVGAENDWTKVVCGFRHTCGIRNGEL
                     YCWGNNFMGELGDGTTEKKETPSRVGNENGWQTVSLGYSHTCGIRSGELYCWGGGYLT
                     YTEGLTYEDFSSPIRIGAENNWKDVAAGGRKPCGLKIGRIYCWDTDKYKTKDVEPVEL
                     DKNSNWKIISGFLYHTCGIRNGELYCWGNNSYAQLGDGTNEDRVEPVKIGTSSGWSVV
                     STGQRHTCGIDGTDLYCWGSNEFGKLGNGEDSFSGDLVQIGSSSGWEAISTGDSHVCG
                     IDNNALFCWGQNYWGELGDGTRINSQIPVLINDSSKWNIITTGNNSTCGIKNDGELSC
                     WGYIGDISSEEIIKWSSVSIFGSGVCGISEGKLFCAEREYQPFVQMDDNDDWNVISSD
                     HDRACGIRSGELYCWGQNMNGRLGIDDEMYIEKPTKIGDLNDWSVINVSSGYICGIRN
                     GTASCWGWNELYFNFDNDENDWTDVSSSEYDICGIKSGKLFCAYYKEDIEQIGNDSDW
                     TSVSVGRNYICGIRDSKLFCWGSNSVGQLGNGKAWELTPVAVVEP"
     gene            17289..>18058
                     /locus_tag="PL056_02420"
     CDS             17289..>18058
                     /locus_tag="PL056_02420"
                     /inference="COORDINATES: protein motif:HMM:NF012632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45903.1"
                     /translation="MKNKLIFIVLFFILLSFLSCGEDGKENAENDSESSNDEDIYEEE
                     DLCGSVDEKCKAGEIKSCVEMNSEIFSDGTAECKDDCSGYNFAGCSVVSGKFDSGTFH
                     VCNISEGKLFCWGNNYYGQLGDGTNIDKNIPVQIGKERDWSFVSAGNNFTCGIRNGEL
                     YCWGDNEYGQLGDGTQEKSNKPIKVDDKKDWTKISSGYYHSCGIRSGKVYCWGNINSL
                     FDKEVTVPFQIGEHDDWEKVESGFFSSCGIRKGELYCWG"
BASE COUNT         6063 a         2911 c         3912 g         5072 t
ORIGIN      
        1 tcaactatga aacagtttca agaatatctt cccacataca gagaattgtt gtttaaaata
       61 agttttataa ctcttttgac ataaatgatg tgatagatat aatccatcag ttaattgaga
      121 gtgaaagtca tagagtcaaa aattttttac acgaggagac atggttaata ttttaaaaat
      181 aaaatctttg cttgacagct ttgatttcac ttcaattttt gtaaatgagc tcggttggtc
      241 tcaccctaaa aacattaaat cactaagcat tcaaataaat gggatggaat tttcaaaaaa
      301 aatgattgct gaacttgcgg gtgttgcagt ttttgaaata agcagctctg acggatcaat
      361 accggatgcc agaacgagag cttcaattta caaggaaatt gtgccgcttc atcatgaaaa
      421 cattctgatt tttgttgata aaagaagagc catgagtctt tggtactggg taaaaaggat
      481 agaaggtaaa ttgtttccaa gagatcatat atttgtgaaa ggtcaaccgg gagatttgtt
      541 tttaagtaaa ataaaatcaa tggtttttga cataagtgat tttgatgatt ttggaaatgt
      601 gtcagttctt caagtgacat caaaattaaa agatgcactt gatgtggaaa aagtcacaaa
      661 aagatttttt aatgaatttc aggaagaaca tgtcagattt attgaatata ttgaaggaat
      721 tgatgatgaa aaagatcgga aatggtatgc atcaattctt cttaacaggt tgatgttcat
      781 atattttctt caaaggaagc tttttcttga taacggaaat gaaaaatatc ttcaaaacaa
      841 acttgagtat ataaaaagtt ttggaaaaga tctttactac gaaaaattcc tgaaaatcct
      901 gttttttgag gggtttgcaa aaccggaaga taaaagaaac agtgaatcaa acaaactgct
      961 tggtaatatt aaatacttga acggaggtct tttccttgag cataggattg agacaagctg
     1021 gcgtaaaata aagataaaag ataaagcttt tgaaaacata ttttctcttt ttgagcgcta
     1081 ttcgtggaac ttaaatgaca ctcccggagg aaaagacaac gaaataaacc cggatgttct
     1141 cggatatatt tttgaaaaat acattaacca gaaagcgttt ggtgcatact acacctgtcc
     1201 tgaaatcaca gaatatctct gtgaaaggac aatcaacagg cttgttcttg aaaaagcgga
     1261 aagagcggga aagaaatttg attctatcgg tgaaatgttg attcatgccg atgaagaact
     1321 cacaaaagaa ctgcttttag actttcttcc aaaattgtca ttgctcgatc ctgcttgcgg
     1381 aagcggtgct tttcttgttg cagcgatgaa aacattaatc aatctttatt ccgctctgat
     1441 aggtaaaata gagtttcttc aagatcagtc attaaaactg tggattaaga aagcaagact
     1501 ggaacatccg agcattgcct attttataaa gaaaaaaatc atcacagata atttatatgg
     1561 tgttgatatt atggaagaag ctgtggaaat tgcaaaactg cgccttttcc ttgcacttgt
     1621 tgcttctgct tcaaaagccg aagaacttga gccgcttcca aacattgatt ttaatattct
     1681 ctccggaaat tctcttattg gtttgatgaa agttgatgac aaagattttg aaaataaaac
     1741 agctaatatt ttcaggatga gatcttataa agaaatactt gatgaaaaaa acaagcttat
     1801 tgatttatac aggcatgcat caatgtatgc ggaagattta aaaagtatga gagattcaat
     1861 tgaaaacgaa aagaaagagt cgtcgttaaa cctgaatgaa attttacatg atatgttctg
     1921 gaaagagctt aaaataaaat ttgaagaggc gacctgggat gataaaaaga attcagaggg
     1981 aaaaacaaag aaaaggaacc tagaaataaa agatattgaa gcactcaagc cgtttcactg
     2041 gggttacgag tttgacagaa taatcaataa gaaagggggt tttgatgcta tcatcactaa
     2101 tccgccatgg gaaatattaa aaccgaatgc taaagaattt tttgaagaac attcagaact
     2161 tgtcaccaaa aagaaaatga caataaaaga gttcgaaaaa gaacagaaaa agcttttaaa
     2221 agatcctgaa atccgcaaat tatggcttga atatctaagc cagtatccgc atgtttcgga
     2281 atatttcaga acttcacctc aatataaaaa tcaaataagc attgtaaatg gcaaaaaagc
     2341 gggaacggat ataaatctgt acaaactttt caccgagcag tgcttcaacc ttcttcgtga
     2401 cggcggatgc tgcggaatcg tcattccaag cgggatttat actgatctcg gcacaaaaca
     2461 gctgcgtgaa atgttgttca atgaaacagc tgtgaccgga cttttctgtt ttgagaatag
     2521 aaaaggaatt tttgagaatg tggacagccg ttttaagttt gttgtgctta gttttgaaaa
     2581 aggcggcaaa acagctgatt tccctgctgc ttttatgcgc caccatgtca aagagcttga
     2641 acggtttccg cgggaagggg caatcaatat ttcagttgat ctcgtgaaga aattatcgcc
     2701 cgattcactc tctgttatgg aattcaaaaa tgatatggat attgtgatag caaagaaaat
     2761 gatgaagttc ccattgctcg gagagaaaat tgaaggaact tggaatctta aacttggaaa
     2821 tgaatttcac atgactaatg acagttacct atttaaaacg cagccgggaa aagggagact
     2881 gccgctttat gaaggaaaga tgattcatca attcgtaaat aattatgcag agccaaaata
     2941 ctggattgan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng gactgactga
     3061 agaggaattt gtccatgtcc tttccacttt tccgattgtt ggcgatgaca taaaacttgc
     3121 ggcacataac gcatttagaa attacgcaaa ggggttgata gaatgaaaaa atttaatctt
     3181 gaattgaatt taattttgtg cataatataa aaattggagg aagcaatgaa aaaatataac
     3241 aacccaccaa tcaaagaagc aatctttgat atcaggttta atgaaagaat tagtgaaggt
     3301 gatattcaaa agatttctga gacctttttt aaagaagttg aaaaagaatt tccggataaa
     3361 aagctacaga gaagttttga agagaatata acaataaacc cagacggaga aagaaaaatt
     3421 gaatcaaaaa atgaaattac cggatattta ttctggtccg gagataaaaa agaagtagtt
     3481 caaacaagaa ttgacggttt tgcatataat attttggaaa aatatgaaaa ttgggaattc
     3541 ttttcaaaaa aggcaatgaa tttgtttaat gttcttaaaa aagttgatac ggagcgaaca
     3601 atttcaagga ttgctttaaa atatataaat gtaatcaatc ttcctctcga caatctgaaa
     3661 attagcgatt atatttcaaa catgccacca atgtgtgatg aatttcccca aacaatgaag
     3721 ggatttattt tgaaaaccca ttttgtcgaa aatgatgtga atataataaa tatagaaaaa
     3781 atactgccgt taaaagacaa cattcttcct tttgttattg atattgatgt gtttgttcag
     3841 aatccttctt ttgaaaaagc agatcttcaa aaaagaatgg aagagttgag gaagacaaaa
     3901 aatcgaattt ttgaattatg tataacagaa aaaacaagga gtttgttcag atgaaatacg
     3961 atattacaat tgacggttct tcagtccaga caaaatctcc agtttcagat tttgctttga
     4021 aatatataga agatgtttca agaaaggaaa cattaaaaca tttttatata ttagaaaatg
     4081 agtttgaaac actcaaagag aaatggaaaa aagaagtgaa gctgacttcg tctatgacag
     4141 aaattataaa taacgaaaac tatcagaaaa taatagagct tggttatgat gtaattgatt
     4201 ttatttatga agaattgaaa aaagaacccg atttctggtt ttatgcacta aaagaaataa
     4261 ccggagctga tcctgtaagg tcaagtaatt acgggaattt aaaggcaatg actcaagatt
     4321 ggatttcatg gtttaaatca aacggtttct gatatgaagc aggaagaact gagtttcctt
     4381 tttccaaatt cccaaaaaga accatttact gaaacaagtc ctgctactaa tagttacaac
     4441 tgcattgcct gggctgcaaa cgacaactca aagtggtact ggccagaaaa tcattgttat
     4501 tggccggaag gtgttgaaaa aaaggaaacg attgattctt ttattaaaat gtttagcacc
     4561 ttcggctatg aaatttgttc tgacggtttg tgcgaagaag gaatagagaa aatagtaatt
     4621 ttttctgatg aaaacgggaa accaacccat gcagcaagac agattgatga aaatttctgg
     4681 acaagtaaac ttggaagaga aattgatgtt tctcatacat tgaatgcaat gtgtaatggc
     4741 gattatggaa acccagccgt ttttatgaag cgccgcaaaa agcaatcgaa ggagtactaa
     4801 atgcccagaa tttttgataa tattgaagaa aaacttctat ccgctcttga gcagtcttta
     4861 cctcttgcgc accgttctga cttctgtgtc ggatatttta atctcagagg ttggaagaaa
     4921 atatcaagtt tcatagataa ttttaaaggt ggggagaaca actgctgccg acttcttgtt
     4981 ggtatgcaga gattgccgga agaagaactg aaacttgctt tatacgaaga agataaaagc
     5041 cttaaccttg atagaccaac tgcaattaaa ctcaagaaaa aacttgccga agatttcaga
     5101 acacagctta cttatggttt tcccactaat gatgatgaag taggtttaag gaaactatca
     5161 gttcaactga aatcagaaaa actgcatgta aaactttatt tatctcatca gcttcatgca
     5221 aagttatatc tggcttttag gaatgatcct attaacccga tcattggata tctcggtagc
     5281 agcaacctta ctttctcagg tttgtcagag cagggagagc ttaatgttga tgttcttgat
     5341 aatgatgctg ccaagaaact ttctaattgg tttaatgacc gatggaatga ccagtggtgc
     5401 attgatatat ccaaggaact tattgaaata attgaggaaa gctgggcgcg cgaaaaacct
     5461 gttccaccat atcatatata tataaaaatt gcatatcatc ttgctcagga ggcaagggca
     5521 ggactgtcag aattcagaat tccgaaagag ttcgggaaca aattattcga ttatcaggcg
     5581 gcggcagtta aaattgcggc acatcatatt aataaacgca atggagtaat aatcggtgat
     5641 gttgtcggac ttggaaaaac tttaatggca acagctgttg caaaagttat gcaggaagac
     5701 agattttctg atacgctaat tatctgtcct aaaaatcttg ttcaaatgtg ggaagactat
     5761 gttgcgaact acagattgat tgcgaaagtt atttccataa caaaagtaaa tgataatttt
     5821 cataatgaaa tgataagata ttcacttgtt cttattgatg aaagtcataa tttgagaaat
     5881 cgtttcggca agagatacaa tgcaattaaa gattacatat caaaaaacaa ttgtaaatgc
     5941 atgctgcttt cagctacacc gtataacaaa acatttcttg acctttcctc acaacttgca
     6001 ctttttctgc cggacgatga ggatatcgga ataagacctg aaaaattaat taaagaaata
     6061 ggtgaaactg agttcataaa aagacatcaa tgtcctttga gatctcttgc tgcttttgaa
     6121 aaaagtgatt ttgctgagga ttggcgtgaa cttatgcggc tttatctcgt tagaagaacc
     6181 agaagtttta tacagaataa ctatgcagag atagattctg aaactggtcg gaaacactta
     6241 acttttgaag atggaacaaa atcatttttt ccaatcagag tgccaaaaac ggtgactttt
     6301 ccttcaaaaa acaaccagta ttcgattctt ttttcagata ctgtagtaaa aacaataaac
     6361 gaattaaagt taccacgata cggtcttggt aattatgttg ctccgactca cgagaaagaa
     6421 ccatctcaaa atgattccaa actgctttca gatttatcac gagccggtaa aagactaatg
     6481 ggtttttgcc gtaccaacct ttttaaaaga ttagaaagtt caggatttgc atttattcag
     6541 tcaattgaaa gacacatact cagaaattat gttttccttt acgcaattga aaacaatctt
     6601 tctcttccgg tcggaactca aaatgcagat tttcttgatc cggaaattaa tgatagcgag
     6661 gaaaaagaaa atataatcaa acttaaaacc gttgatgact ttaaaaatca ggcaaagccg
     6721 atatatgata agtatttaaa acatcagaag aattatttta agtggattaa atcggaatat
     6781 ttcatagaaa accttaagaa agaccttatt tatgatgccg aaaaacttat taaagtgttg
     6841 gaaatatgcg gagggtggaa agaatgtgaa gacccgaaac ttgaagaatt gaaaaaactt
     6901 ataaataaaa cacataaaaa cgataaagtt ctgatattta ctcaatttgc tgatactgtt
     6961 gaatatattg aaaaagcatt aaagaataaa gatgattccg atattttagg tgttacgggt
     7021 gattcggatg acccgacaat gtgtgcatgg agattcagtc cggtaagcaa taaaaaagaa
     7081 aatgttataa aaaaagatca ggaagtgagg attcttatct caaccgatgt tttaagtgaa
     7141 ggacagaatt tacaagatgc caatattatt gttaattttg atcttccttg ggccattata
     7201 agattaattc aaagggcggg ccgtattgat cgtatcgggc aaagatctga taaaattatt
     7261 tgttattctt ttctgccggc agacggagtt gaaaatataa tacatctgcg cagaagggtt
     7321 agagaacggc tcaatcaaaa tgcagaggtt gtagggacag atgaagcgtt ttttgaagat
     7381 gaaaatacaa aaagaaaaat tgtggatttg tatcatgaaa aagcaggtgt acttgatgat
     7441 gatgagaatg aagttgattt gacttcttat gcttatgaaa tatggaaaaa agcaattgat
     7501 tctgacccgg atcttaagaa aattattcct gaaattccta atgtggttta ttcaacaaaa
     7561 caatctgaca agaacgaagg agtgatcgtt tatgttaaaa ctcaagaggg gactgatgca
     7621 cttgcattaa ttgacaagaa tggagagagc ataactgaat cccaatatga gattttaaaa
     7681 gtggctcaat gtgaaccctt gactcaagct gttgaaaaat ttgaaaatca ccatgaattg
     7741 gtaaaaaaag gagtgaaaat aattgcagaa gaggaaaggt ctataggtgg acaacttgga
     7801 agatctacag gtacaagatt caaaatttac gaaaaactga agaattatgc acaaaagtac
     7861 aaagacaccg ttttagacat tccttctctt cacaaagtag ttgatgaaat atataacaat
     7921 ccgcttaaac aatctgcgat agagattatt aataggcaga ttaaggcttc aatttcagat
     7981 cagaatcttg tcgatttagt tatttcttta agagaagaag ataaattgtg tgcaattcaa
     8041 ggggaaaaag aaaggaaaga aactcagatt atatgttcaa tgggaattag ggaataatca
     8101 tatcatatgt caccgtacat tcaaaaactt gacaacagac cttttttgca tataaaaaga
     8161 cggcttttaa gttctgtgga atttttaagg ttttttacta ttgatggagg atagtttggc
     8221 tcatcacaag tcggcgaaaa aaagaattaa gacaaatgaa aagaaaagac tgtacaaccg
     8281 ttcaataaaa tccaaagtga aaactttcac ccgttatgca gtcgaaacaa tagaagcggc
     8341 tgaaaagaac actgccggtg ctgctgaaat gaaaactgat gctgcagcaa agcttacaaa
     8401 agctgtaagc gagattcaga aagctgtcag caagggcgtt cttcagaaga atacggcagg
     8461 aagaaaagta ggacggctga cgattcgttt taacaaggtt ttcgctgccc agtaatttga
     8521 gatactctct cgtaatcgaa aacatttcct caatatttac tgctgaaaat tcaaaagctg
     8581 ttttaaccgg tggtgatctt gccattgcgt cagacatgtc cggaaagatc tgttttgcag
     8641 gaaaaaagag cgaattgctg aaaattgaca gttcatacag ggacaattct gaatcggttg
     8701 tcgatgcagg gaacagaaca gtattccccg gtcttgttga cagccacaca caccttgttt
     8761 ttgccgcaac gagagaagat gaatttgaaa tgaaggcaag aggagccaca tacgaagaaa
     8821 tagcttctgc cggcggagga attctcaaca gtgcaaaaaa gacagcatcc gcgacacttg
     8881 atgaaattgt ctttcagtcg gcaaaacggc ttgagcaggt aatttcatac ggtgtaacca
     8941 caattgagat aaaatcgggt tacggacttg atcttgaaag tgagttgaaa cttcttaaag
     9001 cggcatctga acttaaaaaa ctgtatccgg tggagatagt tccgacattt ctgggtgcac
     9061 atgcatatcc cgagaagttt aagaacgacc atgacggata tatagatctt ataataaatg
     9121 aaatgcttcc tgaagttaag aatcagaaac ttgctgaatt tgttgatgtt ttctgtgagg
     9181 aaggttattt ttcgcttaaa gagactcaaa agatattaag agcggcatca gatttgggat
     9241 tcggtctgaa gcttcatgcc gatgaattca attctctggg cgggactgaa cttgctgcgg
     9301 aaatgggtgc ggcatcagtt gaccatcttg aggagataac ggaagaaggg ataagaaaac
     9361 ttgcagattc aggaactgtt gcgggagttc tgcctgttac atcggttttt tcacggcttc
     9421 catttgctcc tgctcagaaa atgatagata acggtgtcac tgtcgctctt gcgacagata
     9481 tgaaccccgg aagctgtatg tgcgggtttc ttccgcttgc ggcaagcatc ggtgcgacac
     9541 agcttagaat gagcgttgaa gattcaatca ggggaataac ggtaaacggt gcaagatcct
     9601 tgaaaagaga gagtgcaaaa ggttcgatct ctgtcggtaa ggatgccgat cttgttataa
     9661 tggacacctc ttcctggata tatcctgtct accattttgg tcacaaccat gttcacaaag
     9721 tattcgtaaa gggaagagcg cttgacttat ttaaggaaaa aaatttaata tgagtgttgt
     9781 tgttttttcc ggaaatgtaa gatgagaagt attccgattt ttattctgtt tgcagtttta
     9841 acggcttcct gcagcagtga cacaaaacct gtcgaaagca aatggcgggt tcttgatgaa
     9901 cagatatatg cacccagaat tgatgaagag actgttttta ccgaccttga cgagcttgaa
     9961 aaacttatgt tcgcaccgac cggtttcttt aaggaaaaga gcgggatgca ctcttttgaa
    10021 cagaaaatgg catacctgac aaacagtgtt gtcaatgagg agaaaagaga acttacactt
    10081 gaagaaacgg tgaaattctc ttcagataaa gatggaaatt tcaagatgag atatcgcaac
    10141 aaccgccacg agggctggag catggtctgg aaagacaact tcctttaccg cagacagctc
    10201 ggcggtgaat atacaaggac tgtgagcatg ggagaacatg tctatcttaa ggaatctctt
    10261 ttcagttcga tcccttctgt ttacgcgatg ctgagaaata atgcgaggat atcgtcacat
    10321 gacagaaaga agataaacgg cagtatggcg acagcagtaa taatatcttt cggtgataaa
    10381 aagataaaaa gagaagagct tcctgagaag cgttatctcc agaatcttca ggggactgaa
    10441 gagatgaaga acgatcagct tatttatgat ctggctgaaa aagagaaaaa gaacataacc
    10501 ggtgaaatga cagtatttgt ggatgacaac tattccgttg taagaatgga gattaaaagt
    10561 tcttttgaac ttaaagcaga gaatgttttc ttttcgatag aaggtgaaag gacattgtca
    10621 aagaaagttg cggaaaaggt tgagatcccc aaatataacg aggaatatca cagaagaaca
    10681 ctggatgctt ctgtaaacat tatgaaggat agaaaaaatg acaaagaacg agatgagcaa
    10741 actgaagaaa gtgatcagca ctctgagtga caaaaaaggg atcaatatta ctgcgttcga
    10801 tgtggacagg cagtcgggat ataccgatta catggttttt gtcacaggga caagcattca
    10861 gcataacaat acaatgagcg aagcgctgat gcgtgaactg aagaccgccg gattcaacaa
    10921 gccgcttgtg gaagggagca gtacagcaaa atggatactc cttgatgccg gcgatgttgt
    10981 ggtgaacata atgcttgacg agctcaggga ttattatgct cttgaagaca tctgggttga
    11041 cagcagtaaa gttgatataa gttcttatct ttcgtaatga aaatcactct catagttgta
    11101 ggaaaactta aaaaaggggc cggttttacg gaacttgacg atttttataa aagccggata
    11161 aaaccttatt cacagcttga gatcatagag ctcaaggaaa agaacgacat cagacttgaa
    11221 acagaatcaa tattaaaatc aataccttcc ggttcgttta ccgttgcgtt gagagaagaa
    11281 ggggtgaaaa tggattcctt agaattttca aaactgctca attccagaaa cgaatctcac
    11341 gggcacataa cttttatcat cggaggagct tacggattcc gcaatgtcaa cgaaaacata
    11401 tcgttatcca ttgctccgtg gacacttccg catcagcttg caagaattgt tctgctcgaa
    11461 cagatctacc gcggactctc aatcatcaaa ggttccggtt accaccacgg gtgagggtta
    11521 aattcttgac tttgtatcat tcatttgcta actttctgtg ataatgaact tttccgaggt
    11581 atcatgatgg gtatcaagaa gctttttgca gtaattttcg taagtgctgt ttttacggct
    11641 caggtatatg cggctcagag aatggatttt atgcttttca acctgagtgc gtcgaacgat
    11701 ccacagaaag atttccttga aatgagcaag gagttcactg ctgtcatggc tccgatgttc
    11761 tttggtgaag cggaaacgag gggctcttca ggttttgaga tagggcttgg ttattcgttg
    11821 acaaaaatca atctcaacgc tgactactgg aagttctcac tcaatgatga gccggtagat
    11881 tcaggggttc ctccgtttta caacgggata gaccttcatg tttcaaaggg tttcggtttc
    11941 ggactgaagc tttacggcaa catgagatac tatgtcctga ctgagatgtt aagcggcggc
    12001 ggcggatttg aatatgcgat caatgaaggg cttaagatgg ctccagacat ttctatcgga
    12061 ggtggatata accgtctttt cggtgcaact gacttcaata tgcagatgat cgagctgcgg
    12121 cttaagattt caaaaacttt tgttgcggga tatcagataa agctgatacc tttcattgca
    12181 tacagccacc ttttttcatg ggcgaagtca ggccggctcg gtggctattt tccacaggga
    12241 acaagtgatc cttcacaggt ttcatcaagt aatcctttca taatcaatga ggatggagag
    12301 ccattttatt tcaaagagga attcatcaat gttgacagaa tattcatcgg attcaaaatg
    12361 gtgagcggtt acttcggttt tgaactcgaa gcagcaatcc ctgtgaacgc aggaaaaact
    12421 ttcagtttca atgcaggcat ttctgcatca atgtagatat gatagatctc cactcacatc
    12481 tcctttccgg acttgatgac ggtcctgaga gtgtggatga ttctcttgaa attattaaat
    12541 atctttcttc tttcgggttt acagagcttg ttccgacacc gcataaattc catgctcttt
    12601 tcaatcctgt tgcttcggaa gtgatcgaaa agatcgagaa tctgaaaaga agcatggtga
    12661 agaggttcag tttcgaatat aactgcaatc tccagtcgat aaaagatctt gaagaccacc
    12721 acgagatatg cagaacctct gacgacagaa aagttattct ggtggaattt gcctcaggag
    12781 tggtgagaag gagcgagatc gaaaacagtg tacagtcact taattttgcc ggctttgtac
    12841 ctctcatggc tcacatcgag agatatttca agaaagatga attctggatc gagttaaaaa
    12901 agaaatacag tgttttgttt cagggttcca tccgcacctt tgcaaagccg aatttcaaca
    12961 ttaagaaaaa gcagcttatc cgtttgatgg aacttgatct gatagataac cttgcaactg
    13021 acatccacaa agtctctcaa cttgtaaaaa tagaaaaagg gcttgatttt attcacaaag
    13081 cttttagaaa ttacgaaaaa gacctcttca ccgataaatt ctgaaaacct tacagaaaaa
    13141 cttgaaaacc tgaaatgtgt caggtatgat tcacctgtca attgttgagg tgtgtcatgg
    13201 atgtgatgaa aaagcttgag attttgagtg acagtgcaaa atacgatgca agttgcgctt
    13261 cttcagggag taacagggca actccgaaag ggggcatcgg caatggcaag caatgcggaa
    13321 tatgccacag ctgggcggat gacggaaggt gcgtttcact ttttaaagtt cttttcacaa
    13381 attattgcat ttatgactgt gcatactgta taaatagaag aagcaacgat atttttagaa
    13441 ctgctttcac tccgaaagag atcgccgata taacaattga attttataag agaaattata
    13501 ttgaagggct gtttctttca agcggcgtga taaagagtcc cgaccacacg atggagctcc
    13561 ttacggaggc gataagaata ttgcgcgagg agtatctttt cggcggttat atccatgtga
    13621 aggcaattcc cggtgcgtca aaagaactca ttgaaaaagc tggtaaaatt gctgacagaa
    13681 tatcggtgaa cattgagctg ccgacaaaaa aatcgctgga actgcttacc gacaaaaaac
    13741 acaccgacat ttttacgccg atgaaactca tcacggaagg tgtctcaaca agtattgctg
    13801 aaagaaaagt gagtcggaaa gccccgaaat tcgcccctgc cgggcagtca acccagatga
    13861 ttataggtgc tacacctgaa acagacctga caattgttaa gttaagtgaa gcgatgtata
    13921 acaaatttga tctgcggcgt gtctattaca gtgcttttat tccggtaaac agtgacttgc
    13981 ggcttccggt gctccgtgaa ccaccgcttc ttcgcgaaca ccggctttat caggcagatt
    14041 ttctgatgcg gcagtatgaa ttttccgctg atgagatcct gagtcctgaa catccgatgc
    14101 ttgatatcgc tcttgatccg aaagcgaact gggcgatgaa taactttcat atttttccgg
    14161 ttgaagtgaa tagagcagat tatcaactgc ttctgcgtgt tccggggatc gggccaagat
    14221 cggctaaaag gatcgtggtt gcaagaagaa gccacaattt aagttttgaa aatctgagat
    14281 ctcttggagt ggtgcttaaa agggcgaggt ttttcataac ctgcaaaggg aaaaggatgc
    14341 cgttcatcag tgaaaaattc gcaactgttt acaaatcact ttctgaaaat catgagccgg
    14401 ggccgtttca acttccactt tttaacacat gaatttatga atacacttta tctttttgac
    14461 ggaactcttg aaacattcat ttcgattatt ccggttctgt gggctatatc tgaaccgtct
    14521 gatcgccttt tatcgcaatc tgaatacact ccggggatct ttgaaaaaac ggtatatctt
    14581 gatgaaagat catctgccga tctccctgtg aaaaacaaca gtattccaaa agaagtaata
    14641 aatgcagttg tttatgcatt ttcttcagga attgtagaag cggttgaaaa aactccgcag
    14701 tatttaaatt acattaaaaa taacggaatt gatgcgatga gtcatctggc ggaaccttcg
    14761 gtcagtgtga tatttaaagc ggcaaggaaa acatcgagtg aggcacataa attaaaagga
    14821 attctaagat ttagaaagct cgggaatatt ctttacggac cgtatttcag caagtgtttt
    14881 gttctgccca tacttgcaga gcattttaag caccgtcttc cgaatgaatc gtggatactg
    14941 cacgacacag aacggaaaac cggagttttc tacaacggaa aaactgttca tcaggcattc
    15001 ttttctcctc gcgaactctt tgaaatcact aatgaatatg aaaaatccgg agttgaagat
    15061 cagtttgaaa cactctggaa aaaatatttt gacacaatag ccattgaata tcgcaaaaat
    15121 cccaaactcc aacagtcatt catgccgaaa cagtactggc gattcatccc cgaagttgca
    15181 aaaagggttc tttgacattt gtgacttttc aattaacata aatagaactt agtgtttaca
    15241 atcctgagag gaagttatga aaaacaagtt gattttcatt gttttatttt tggttttatt
    15301 gagctttctg tcgtgcggaa acactaaaac cggatctgaa aataacaatg aaaatcctga
    15361 tagtgaaatt ataaacgaaa gcgaatggtc tgttcttgct gctggcggac atcatacctg
    15421 cggaataaag aacggtgaac ttttctgctg gggggcaaat tatatcggac aattcggtaa
    15481 cggaacacag acagataata caactccgtt aaaaataggc atcgaaaatg actggacaga
    15541 tgtttcagcc ggttatgagc atacttgcgc aattcggaat ggtgaacttt actgttgggg
    15601 caatctttat tttgcttacc ttattcccgg tgttgatgaa gaaatgttaa gtcctggcaa
    15661 agttggggca gaaaacgact ggacaaaggt tgtctgcggt ttcaggcata cttgcggaat
    15721 ccgtaatgga gagctttact gctgggggaa taatttcatg ggcgagcttg gagatggcac
    15781 gactgaaaaa aaagaaacac catcgcgagt aggaaatgaa aatggatggc agactgtttc
    15841 gttaggatat tcacatactt gcggaataag gagcggcgag ctttactgct ggggcggagg
    15901 atatctgaca tacacagagg gattgaccta tgaggatttc agttctccga taagaatcgg
    15961 tgcagaaaac aactggaaag atgttgctgc aggcggacgc aaaccgtgcg gactaaaaat
    16021 tggccggatt tactgctggg ataccgacaa atataaaact aaagatgttg aaccggtcga
    16081 attagataaa aacagcaatt ggaagataat atcaggtttc ctttatcata cctgcggcat
    16141 taggaatggc gaactctact gctggggaaa taattcttat gctcagcttg gtgatggaac
    16201 aaacgaagac agagtagaac ctgtcaaaat aggcaccagt tctgggtggt cagttgtttc
    16261 tacaggacag cgtcatacct gtggaatcga tggaactgat ctttactgtt ggggcagtaa
    16321 cgagtttggg aaacttggaa acggagaaga ttcattttca ggagatctgg ttcagattgg
    16381 aagttcatcg ggatgggaag cgatttcaac aggtgattca catgtttgtg gaatagataa
    16441 taacgcttta ttctgctggg gacagaatta ttggggagaa cttggtgacg gaacacgaat
    16501 taacagtcag attcctgttt taataaatga ttcctccaaa tggaacataa taacaactgg
    16561 aaataatagt acttgcggaa taaaaaacga tggagaactc agttgctggg gttatatcgg
    16621 agatatttca agtgaagaaa ttattaaatg gtcatcagtt tcaattttcg gttcaggtgt
    16681 atgcggaata agcgaaggaa aactgttctg cgccgaaaga gagtaccagc catttgttca
    16741 gatggatgat aatgatgact ggaatgtaat ttcatcagat catgatcgtg cctgtggaat
    16801 acgcagcggc gaactctatt gctggggtca gaatatgaat ggaagattgg gcattgatga
    16861 tgaaatgtat attgaaaaac ctacaaaaat aggtgatctc aatgattggt cggttattaa
    16921 tgtgagttca ggttatattt gcgggatcag gaacggaacg gcttcctgct ggggatggaa
    16981 cgaactttat tttaattttg ataatgatga aaacgactgg acagatgttt cttccagtga
    17041 atatgatatc tgcggaatta aatctggaaa actgttttgt gcatattata aagaagatat
    17101 tgaacagatt ggaaatgatt ctgactggac atcggtttcg gtaggaagaa attacatttg
    17161 cggaataaga gatagtaaat tattttgctg gggcagcaac agtgttggtc agctgggaaa
    17221 cggcaaagcg tgggaactga ctcccgttgc ggttgtggaa ccgtgatgaa aaacaaggag
    17281 aggaagttat gaaaaacaag ttaattttca ttgttctatt tttcatttta ttgagctttc
    17341 tgtcgtgcgg agaagatggc aaagaaaatg cagaaaatga ttctgagagc agtaatgacg
    17401 aagatattta tgaagaagaa gatttgtgcg gttctgttga tgaaaaatgc aaagccggtg
    17461 aaataaaatc atgcgttgag atgaattctg agattttttc cgatggaact gctgaatgta
    17521 aagatgattg ttccggttat aactttgctg gatgttcagt tgtttcgggt aaatttgatt
    17581 caggtacttt tcatgtctgt aatatatcgg aaggcaagtt gttttgctgg ggtaacaact
    17641 attatggaca acttggcgat ggaacaaata ttgataaaaa tattccagtt cagattggaa
    17701 aagagagaga ttggtctttt gtttctgccg gaaacaattt tacctgcgga ataagaaatg
    17761 gagagcttta ctgctgggga gataacgagt atggacaact tggcgatgga acacaggaga
    17821 aaagcaataa accgatcaag gtggatgaca aaaaagattg gacaaaaata agttcgggat
    17881 attatcactc ctgcggaata agatcaggta aagtttactg ttggggaaat ataaactctc
    17941 tttttgataa ggaagtaaca gttccttttc aaattggaga acatgatgac tgggaaaagg
    18001 tggagtctgg ttttttttct tcatgcggaa ttcgtaaagg agagctttac tgctgggg
//
LOCUS       DAOOYB010000023        17954 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_3022_length_17954_cov_13.604559, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000023 DAOOYB010000000
VERSION     DAOOYB010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 17954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17954
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3022_length_17954_cov_13.604559"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="PL056_02425"
     CDS             complement(<1..332)
                     /locus_tag="PL056_02425"
                     /inference="COORDINATES: protein motif:HMM:NF020090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45904.1"
                     /translation="MNRPSPIIGTFEKAPVIYIWGDPLDYTLSPFFQEYVLGACGHRV
                     IYKVFRGNAEEFKQLLMSRKCIGANITSPHKQKAVEICEELSDRASKAGSVNTIYKKD
                     GKLCGDST"
     gene            complement(298..1101)
                     /locus_tag="PL056_02430"
     CDS             complement(298..1101)
                     /locus_tag="PL056_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45905.1"
                     /translation="MYFRVYHKKSKQPIATFNERTIVDFCINNKESVKNYYFSTGGQK
                     DLMEWEVFAVKHSELFEKKADERDKKEFGEVKDEGEIKIKSRGSRIPFMIVLTALFFA
                     SGVAYYLYLEQQKEFEEMTRKSLLASQEKKAAIVEKLEKRAVVESDTKFTAAVPSEKE
                     LNETWSSNKGKMEKSLGFDEIKEHMDSYLPNLKECFVKRSKAGDNSLRGMINMKIRVS
                     GDGAVRDVIFTDEKYQATLFGDCIISAMKSKPFKMFKSREQTFSYYWNL"
     gene            complement(1109..2311)
                     /locus_tag="PL056_02435"
     CDS             complement(1109..2311)
                     /locus_tag="PL056_02435"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HPM45906.1"
                     /translation="MGKYSSLVKESGAAADKMKFRFISGKYSGGEFFIRDGQKLSIGR
                     DISSDVAIVDSKVSRNHATIISRAGRIFIEDHNSTNGTFVNGEKILPSSPVEIHNGFK
                     ISIGDSVIAVGEGAADNNEKVERSVKEDTPSGQIGVSSTQNTGKVMPPLDEYDEDEPL
                     TLDAIAEKAEKVENNKISVAKVALKKGGPATTSKTVPDSAVASSKGSLSAIDPVDLLK
                     LLSQSSSSGYLIVNISSPFEEKIEIAIGRAGIVASECISSRNFSQEKALSRFVLAKDG
                     EYEFKVDEAPKREEINRFLEDVFMEISTQRGLLARYRKIINADQLRFMIPITGKLSDL
                     SKPELETLQFMVNTREVLPYLNMFPDNDDFILLSEILKFIDLGILFGDNNEEDHIENL
                     VPDDILQI"
     gene            complement(2442..2873)
                     /locus_tag="PL056_02440"
     CDS             complement(2442..2873)
                     /locus_tag="PL056_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45907.1"
                     /translation="MKTTLLKTFLAITLILLLAVTYLFRQESEMNRKLNEEIIQLKKE
                     KIFVLSEHEECLKHKEMSLKRELIDKYIDSMIVLVNKIEKGTNPSEKELSDFNDRVNF
                     ILENMELAGVSKEEATQYLLFVSSARKSIEQYVNGNSGKKE"
     gene            3038..3601
                     /locus_tag="PL056_02445"
     CDS             3038..3601
                     /locus_tag="PL056_02445"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPM45908.1"
                     /translation="MSVKNGDKIAVHYTGWLEDGTKFDSSVDSGETFKFQVGEGMVIK
                     GFDDAVIGMEIGNKKKITISPENGYGERNEKYVITVEKKSFPADAAIETGSIFEMHSD
                     HGDVIPVRVVEIKDENVVLDANHPLAGEALTFEIELIETGVDIPDYEHHHHGCCCGNE
                     DCDDDCDEECDEDDENCGCGCGCDSKK"
     gene            complement(3701..5272)
                     /locus_tag="PL056_02450"
     CDS             complement(3701..5272)
                     /locus_tag="PL056_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45909.1"
                     /translation="MKKMILLAIVVAAVFMFTACPKAGEPEPKEKVKLSFHVMSKCPY
                     GAQVVAGVKPALDKLGKAVDFELNYIGNEKEGKWLPMHGEPELKGDKIAVCAKKYLPE
                     KYMDLLFCMSKDKEFRKIPDNFDACITETKLSGDKVKACADGEEGTTLVVESYKASKA
                     KGASGSPTIYLNDQPYQGGRTGNDFLRSICEKFTTEKPKACSEIPEPTKVKVTMISDK
                     RCPQCQAERLAQNLKGVFPGLEAEILDYADEKGKAEYQKYAAKGHTLLPIIAFSAEAK
                     KDEGYAKIQKWIQEVDDKLILRVGAKFDPTKEICDNKTDDTGNGKIDCDDDDCKEELI
                     CRTEIKKKLDVFVMSQCPYGTMALDAMAEILEMFKEDKIDFNINFIATETEPGKFQAL
                     HGQPEVDENIRELCAIKHYPKNYMDYILCRNKNIRSAEWKECAKGAIKPDVMEKCFTG
                     GEGVKLHSENIKIANAMGIGGSPTWIINNKHKASGVSAADIQRNLCTHNPDLKGCSKT
                     VKPQDKPAPKGGCGS"
     gene            complement(5380..7413)
                     /locus_tag="PL056_02455"
     CDS             complement(5380..7413)
                     /locus_tag="PL056_02455"
                     /inference="COORDINATES: protein motif:HMM:NF040596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MXAN_2562 family outer membrane beta-barrel
                     protein"
                     /protein_id="HPM45910.1"
                     /translation="MNKITVFVSIIFFLSTVLADDAEYLEKVRLEPEHGSLNYEGEIK
                     ILFDLPKDDLVEKVYLSYTQGVEEHIFSSTEVSGSKLGYLNNSLTLKGSELYELLESL
                     RTSVNFRNITAAADTDDDDVLIDDDAEESDDDTVNDEDETYDEELPDDDSGTGTDRSW
                     ADKTYDIKLTVELDNSGNNEDNETDDSDTAPVTTTTIPEKVTKTLKITFDNTAPQSPE
                     INDDDTVNPEGGDRRIIIYVTPPVDPDDKEERDEIGKYHATLSGLFDRDGTEVQSELK
                     FTTEVESSSYDEEWEFSLSGKEGLELINNDSGLDKYVYTIRIYAEDIAGNSDPEKYVE
                     TAGSAVTTYGYWKNYKEKGGRDDGGFCFVATAGFGSYFHPQVKILRDFRDLVLAKFSA
                     GRSFIKAYYEYGKMPARIIAESQFLRAVTRTLLIPFVITGWLFTTTYGQIILLMWLFL
                     IPVFILRRKAVVLLMVIAGIFVLDPQELKGVDGEFSFNSSFYYPSKIDSEADGAFKEI
                     GGDGLRYLPSLTFGFKVPVLENYIRWSFIGGIGYTRFKGTSVKADGEKSSDESAMHFI
                     PLMGEMKLRPGYDFPLWPYATIGIDYYIWWIREKGETSEDGGTFGFHGSFGLMISLNW
                     MDDSASRKLEELSGITNTSLFVHYRLEKINDFGKEKSFDLSGSRFEFGIVFEF"
     gene            complement(7406..8428)
                     /locus_tag="PL056_02460"
     CDS             complement(7406..8428)
                     /locus_tag="PL056_02460"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013638763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HPM45911.1"
                     /translation="MKKFNVAIVGATGLVGQEFISVLEERKFPVNELIPYASERSIGE
                     TVKFRNKEIDVIPMTVESIQSKNIDIALFSAGSAIALEFAPVFAQKGVYVIDNSSAFR
                     MNDSVPLIVPEVNGDLVKPDGSKIIANPNCSTIQLVYFLKVVNDLFKIKKVVVSTYQS
                     VSGAGKKGIDELREQVTALFSGRSVAPAAFPQRIAFNLIPQIGYFHSDGYCEEEVKMI
                     NESRKILSIPDLDFDVTTVRVPTFFSHAETVYVDLEKKCDVELVRNKISEHPEMVVMD
                     DFEQLIYPTLVDSSGKNEVFVGRLRTGREDGKRLSAWIVADNVRKGAAYNAVRIAETL
                     IGGMDE"
     gene            8639..10621
                     /locus_tag="PL056_02465"
     CDS             8639..10621
                     /locus_tag="PL056_02465"
                     /inference="COORDINATES: protein motif:HMM:NF039815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45912.1"
                     /translation="MKKFFSVILITIFFVACSSGKSSINDEDIATGETKDENQQLPTS
                     DDEPGEVEDDMSDDLSDDMSDEALAESDEDDSPLPDEDTAFYEDKCDCFGSEYTIPEQ
                     FRGIDGWCKQDADSDGLPNCIEAVDGELTNSDSDADPDYLDTDSDDDGIPDSIEGLED
                     IDKDGIPNYRDTDSDGDGYSDSAECPTLPCRDTDGDSIPDYVDFDSDNDGLTDKEEKD
                     LNTDPLKQDSDDDGFDDLSEIAYGSDPLDKADGIPEDDFYVKLPFEAPADEIRILKFK
                     TNVEKADVLILFDLSDSMSQESTNVKNGISSEIIGGISANITDVAFGLATFDDWKGLS
                     RYSDPTEGTNYSGTGTLQHTDSIFSLVQPVTTDMSEITGSINGIQTGSLCGWEPHHEA
                     LYQVATGAGYTGDFYFETTYVSTNKCDVVGNYIPEIPAANCTGHEGNIGGGCFRDDAM
                     PIIIMLSDEAFTNFGAHFTWNIPYHTTEEAIAALNAINAKFIGIDSWSEETFWTTAPE
                     NDFKAISTATGSVDATTGDPFFYKIGSDGTGLSDQIADAVFELTTNIKLDVWTARESV
                     ANPELVDTSLFIKALIPDSSDPVNAYFSKDTTTFYLVNPGAKVNFEIKFHNDFYEPDK
                     AEATVFRAKINVLGDGALLDTREVVILVPGIHSRDN"
     gene            complement(10618..10887)
                     /locus_tag="PL056_02470"
     CDS             complement(10618..10887)
                     /locus_tag="PL056_02470"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HPM45913.1"
                     /translation="MRYKILFTKASVKMLQKIDFPIQKLIKIKLDILSNDFNSLENNL
                     KQLKGDKKIYRLRVADYRVIFEKKDAELVILVIRIGHRKEVYFDI"
     gene            complement(10887..11117)
                     /locus_tag="PL056_02475"
     CDS             complement(10887..11117)
                     /locus_tag="PL056_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45914.1"
                     /translation="MSRIKTTFTLDEDVVAELETFSKGLSKKKSHIIETALSIYFDMM
                     DSHLAEKILKDVESGKEELVDSEKVYKDLNIN"
     gene            complement(11215..11880)
                     /gene="radC"
                     /locus_tag="PL056_02480"
     CDS             complement(11215..11880)
                     /gene="radC"
                     /locus_tag="PL056_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HPM45915.1"
                     /translation="MKQIHELYSDDKPREKMLSKGSAALKNRELIALILGSGQKGMPV
                     MTISRNIENMLEKEGVGGIALEKLTNIEGVGNAKACQIAAAFELAKRFYVESEKVNIK
                     VSRDVADLLDGYASKQQEHFILITLDGANNVIKVRVIFIGTLNKSMVHPREVFAPALA
                     DRAASIVIAHNHPSGNPEPSVEDLLMTRKLKEAGEIIGIEIIDHVIITKKNHYSFQGN
                     GKM"
     gene            complement(12012..12506)
                     /locus_tag="PL056_02485"
     CDS             complement(12012..12506)
                     /locus_tag="PL056_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45916.1"
                     /translation="MKCDIFYDTSKLSHSEQEALLKKAHFICENWWFDKLDCSISFAR
                     EQVKNISFEEAMEHFVEGALMNVIERNQILLDKPCLEVGFRSMESPVDYFLWIIVPLE
                     NADDIVKGMVKEKSDDGINESVPDVILNDVLIEDLEFQPDFDGDKEQCRKKENSRNLG
                     KCSK"
     gene            complement(12503..13048)
                     /locus_tag="PL056_02490"
     CDS             complement(12503..13048)
                     /locus_tag="PL056_02490"
                     /inference="COORDINATES: protein motif:HMM:NF014045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HPM45917.1"
                     /translation="MSIKTSILIDGGYLREILNRKRGHRYYPKPEEVLNFCNSLIKDE
                     EVLYRIFFYDAKPDNDNNSDYFFNKIESQPFFALRMGRLVNHTPKGGPLNYVQKGVDM
                     KIGLDIASMAYKKLVERIVLITNDTDLIPAIKLARINGIQVRIINLDSIHSLFQAHSD
                     MWEKIEIEDHFSESLKKGKKQ"
     gene            complement(13197..16565)
                     /locus_tag="PL056_02495"
     CDS             complement(13197..16565)
                     /locus_tag="PL056_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HPM45918.1"
                     /translation="MRKIKIFSIVSVLTVSTVFLFAGGFWPEVQNNSFRTGRAEMSGA
                     VKEPGILWKYYRGGNLSPNNVAVDENGNIFFSINGVVKAVDKNLNLLWVSENFGASRI
                     FKIIDLNNDGTKELVVAGNLGIRIINKENGSIHSIIPTESPTFAKFADVNEDGFVDLV
                     VRGRWNTKDVRAYDFSSGVTTPQMIWQITENIEGYGFELTFGDLNNDGEKELITDRIS
                     GGLISVFKAIDGTLIRDKARVLDGNYAYGFNQIINVDNDDQNEFIFTGRTSSESDKGS
                     YSITVYDFAEDAVQWQYEYGFNTVNKGFRMVPGSVADFNGDGVIDVVVSVFNNTLEGA
                     TDLDGVNAPDIWTTLIYRGDNGLFQARLDNTYLEGVADLNGDGVKEFIIKSAPDSSKK
                     LRIYSTISAYSFDGDFQNLWSKDKVRVVTKTPVDSEDVGVIYAGDVPAIVRVGSNDAV
                     LLIEDINRDGVGDKLISFSGVASTPVVSDTAYLKDGKSYGFIDSTDFHLYLTGNDGFI
                     HIFDRGATLSNELVIETGTFSGDSQFINSSTGGKILSTISTNSHVLLNSENSDLENPP
                     TVEWTQTNQSAQPLFAFDSNGNGRHEFISLFTDSEGYTDIYLHSSNGGLLWGWTVGKV
                     VTNPANFIAGDFDGDGLKDIAFTFTTEEEGAMLYALRGIDGSEIGSHDPQVDITGYYN
                     NTTVLLGDINDDGSDDLFLGHSFTSEFINGNMTRILKFSFSSWPNNSIAADLNGNETA
                     EIFGNHRTGSFKRTIDNSGNELWTIDISWDQDSLFQYSALYPGIAKIDSDEGFDIVLG
                     GKFGDVSAYSGADGSVLWRKCLNNGTVTDISVSVIPTSTLCGGTNLSNIVTGDIDGNG
                     GDDFVVGDKLGNLYVVDINGELVWTMKFDGVIGNPILADVNGDGKIEILIGAGDGFLY
                     AIGQKVAVPAPAFVRDVAVIDGEIASNTDIDEMLTGESYGGLWEGVPSAAKYHVKLTD
                     QDETVLKETMVTGANQVVFDNLEVEIGDTIYLNVKVINSGGYESGWTTSDGVTIVEED
                     MSDEVSTESDEETDDDEATDDLSDEALAESDDDLTTDEDDAATDDLSDEALAESEEAT
                     DDDTDLSDEALAESDEDDEEIPSKNESGCGCSIL"
     gene            17221..17907
                     /locus_tag="PL056_02500"
     CDS             17221..17907
                     /locus_tag="PL056_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019704047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HPM45919.1"
                     /translation="MKEAAILVDGAFFLKRYFAIVSKVHPPNVIADNLYATLLNHLYS
                     FRDIEGTYSLYRIFFYDCPPLEKKVRHPLTGRNIDYSKTELVSFRKELHNELKKKRKL
                     ALRLGHLKDESWVIEKNRLKKLLKKEITIDDLTEEDILHSIKQKSIDMKIGIDIASLA
                     YKKLVKKIILVAGDSDFVPAAKLARREGVDFVLDPMWNHIAEDLNEHIDGLKSYFPNP
                     LKRQGFFPKS"
BASE COUNT         5041 a         4097 c         3310 g         5506 t
ORIGIN      
        1 tctgtgctgt caccacaaag ttttccatcc tttttatata ttgtgttgac acttccggct
       61 ttagatgccc tgtcgcttaa ttcctcacag atctcaaccg ctttctgctt atgcggggaa
      121 gtaatatttg ctccaataca ttttctggac atgagaagtt gcttaaattc ttctgcgttt
      181 cctctgaaaa ccttatagat gaccctatgt ccgcaagcac caagtacata ttcctgaaaa
      241 aacggcgaaa gagtataatc gaggggatcc ccccagatat atatgacggg agctttttca
      301 aaggttccaa taataggaga aggtctgttc acgacttttg aacatcttaa aaggttttga
      361 tttcattgca gatattatgc agtccccaaa aagtgttgcc tgatatttct catcggtgaa
      421 gatcacatct ctcacagctc catccccgct gaccctgatt ttcatgttta tcatccctct
      481 taatgaatta tcaccggctt tcgagcgttt gacaaaacat tctttgagat tgggaagata
      541 tgaatccata tgttctttta tttcatcaaa accaagagat ttttccattt ttcccttgtt
      601 tgagctccat gtttcattca gctctttttc tgaaggaact gccgccgtaa attttgtatc
      661 tgattcgaca accgctcttt tttcaagctt ttcaacaatc gcagctttct tttcctgtga
      721 tgccagaaga gactttcttg tcatttcttc aaactctttc tgctgttcaa gataaaggta
      781 atatgcaacc cccgaagcaa aaaagagcgc agtaagaaca atcatgaaag ggatccttga
      841 acctctgctt tttattttta tttcaccttc atcttttact tcaccaaact cttttttgtc
      901 tctttcatcg gctttctttt caaacagctc cgaatgttta accgcaaaaa cctcccattc
      961 catgagatct ttctgtccgc ccgttgaaaa ataatagttt tttacggact ctttgttgtt
     1021 tatgcagaaa tcaacgatgg ttctttcatt gaaagtagct atcggctgtt ttgatttttt
     1081 gtgatagact cgaaaataca ttgctcacct aaatttgaag gatatcgtca ggaaccagat
     1141 tttcaatatg gtcttcttcg ttgttgtctc cgaaaagtat tccaaggtca ataaatttca
     1201 ggatctccga aagaagaatg aagtcatcat tatccgggaa catgttaaga tatggcagaa
     1261 cctctctggt attgaccatg aactgaaggg tttcgagctc gggctttgaa aggtctgaaa
     1321 gcttgcctgt gatagggatc atgaacctga gctggtcggc atttatgatc ttcctgtatc
     1381 tcgcaagcag ccctctctgg gtagatattt ccatgaaaac atcttcaagg aatctgttga
     1441 tctcctctct tttgggggct tcgtcgactt tgaactcata ttcaccgtcc tttgcgagaa
     1501 caaatctcga aagggctttt tcctgtgaaa agtttcttga gctgatgcat tcagatgcca
     1561 caatacccgc cctgcctatt gctatctcga tcttttcttc aaaaggtgaa gatatattta
     1621 caatgaggta tccggaagaa gaagactgcg aaagaagttt cagaagatcc accggatcaa
     1681 tggcggacaa acttcctttt gatgaagcca ccgctgaatc aggaacggtt tttgacgttg
     1741 ttgccggacc gccttttttc agagccactt ttgcaacgct gatcttgtta ttttcaactt
     1801 tttctgcctt ttcggctatt gcatcaagtg tcagcggctc atcttcatca tattcatcaa
     1861 gaggaggcat gacctttcct gtattctgtg tcgaagaaac tccaatctgt ccggaaggag
     1921 tatcctcttt tacagatctt tccacttttt cattattatc tgcagcacct tctccgacag
     1981 ctatgacaga atctccgatg cttatcttaa aaccgttgtg gatctctacc ggagaagagg
     2041 gaagaatctt ctctccgttt acgaatgtac cgttcgtgct gttgtgatct tcgatgaata
     2101 tcctgcctgc ccttgaaatt attgttgcgt gatttctgct gacctttgaa tccacaattg
     2161 caacatcaga ggagatatcc ctgccgatcg aaagcttctg tccatcgcgg ataaagaatt
     2221 caccgccaga atattttccg cttatgaaac ggaacttcat tttatcagca gccgctcccg
     2281 attccttgac caacgagctg tattttccca tttttaatcc tcacgaacac cggatttact
     2341 tttgcaatgc tataaaaata tattgttttt tcaagaaaaa cctcaaaaaa aacttaaaag
     2401 aagtttgaaa atcggggcgt tgcaaagata attcgccctg ttcactcctt tttaccgcta
     2461 tttccattta catactgctc tatcgatttt cgtgccgagc ttacaaaaag aaggtattga
     2521 gtcgcctctt cttttgaaac gccggcaagc tccatatttt cgagtataaa gttaactctg
     2581 tcattaaaat cggacagttc cttttctgaa ggatttgtcc ctttttcgat cttgttgaca
     2641 agaactatca tagaatcaat atatttatca atcagttccc ttttcagtga catttccttg
     2701 tgtttaagac attcttcgtg ctcggaaaga acaaagatct tttccttttt aagctgaatt
     2761 atctcttcat tcagttttct gttcatctca gattcctgcc ggaataaata agtaacggca
     2821 agaagaagta ttaaggtgat cgccagaaat gtctttaaaa gagttgtctt catttaactg
     2881 ctccgcaata aagttcgcgc atatattaca gccatcatgt tttttttcaa attaaatgtc
     2941 ttaaaacaat tccttgcgat ttaaagctgt tttaataaca tagcttttca gaaaaggttg
     3001 ctttttcagg tttttttaat tttttatgga gaaattaatg agcgttaaaa atggagacaa
     3061 gatcgctgtt cattatactg gatggcttga agacggtaca aagtttgatt cttcggtcga
     3121 ttcaggcgag acatttaagt ttcaggtcgg agagggaatg gttatcaaag gttttgacga
     3181 tgccgtaatc ggaatggaga tcggaaacaa aaaaaagata accatatctc ccgaaaatgg
     3241 atacggcgaa agaaacgaaa aatatgtgat aacagttgaa aaaaagagct tccccgctga
     3301 tgcggcaatt gaaaccggct caatttttga aatgcattcc gatcacggag atgtaattcc
     3361 tgtcagagta gttgaaataa aggatgaaaa tgtagttctc gatgccaacc atcctcttgc
     3421 aggagaagct cttactttcg agatagagct tattgaaacc ggtgttgaca ttcccgatta
     3481 cgaacatcat catcacggat gctgctgtgg aaatgaagat tgtgatgacg actgcgatga
     3541 agaatgtgat gaagatgatg agaactgcgg ctgcggttgc ggatgcgact caaaaaaata
     3601 gttccggcag ccaatcttta aataaatctc aaaatataaa tctgaacaaa taaaaaaggc
     3661 ggtccgaaga ccgccctgta attattcatc tcagatataa ctaacttccg caaccgcctt
     3721 tcggagccgg tttgtcctgg ggttttacag ttttggaaca tcctttgaga tcaggattgt
     3781 gtgtgcagag attacgctga atgtcagccg ctgaaactcc gcttgcctta tgtttattgt
     3841 tgatgatcca tgtcggactt ccgccgatac ccattgcatt tgcgatcttt atgttttcgc
     3901 tgtgaagctt aacaccttcg ccgccggtga aacatttttc cataacatcc ggctttatag
     3961 cgccttttgc gcactctttc cattcagcac tgcggatatt cttgtttctg cagagaatgt
     4021 agtccatgta atttttcgga taatgcttta ttgcgcaaag ctctctgatg ttctcgtcaa
     4081 cttccggctg accatgaagt gcctgaaatt ttcctggctc tgtttcagtc gcaatgaaat
     4141 tgatgttgaa atcaattttg tcttccttga acatttcaag tatctctgcc atcgcatcaa
     4201 gcgccattgt tccgtaagga cactggctca taacaaacac atcaagtttt ttctttattt
     4261 ccgtacggca gatgagctct tctttacagt catcatcatc acagtctatt ttgccgtttc
     4321 ctgtgtcatc tgttttgttg tcacagatct cttttgtcgg gtcgaatttt gcaccgactc
     4381 tcaaaatgag cttgtcgtca acttcctgga tccatttctg tatttttgca tatccttcat
     4441 ctttcttagc ttctgcactg aaagcaatta tcggaaggag tgtgtgtccc tttgccgcat
     4501 atttctgata ttcagctttc cctttttcgt ctgcataatc aagtatttcc gcttcaagac
     4561 ccgggaatac gcctttgagg ttctgcgcaa gccgttctgc ctgacactga ggacatcttt
     4621 tgtcagagat catggttact ttcacttttg tcggctcagg aatttctgaa catgctttcg
     4681 gtttttcagt tgtgaacttt tcacagatag accttaagaa atcatttcct gttcttccgc
     4741 cctgatacgg ctgatcattg agatagatag tgggtgagcc gcttgcgccc tttgcctttg
     4801 atgccttata actttcaaca acaagtgtag ttccttcttc accatcagca caggccttaa
     4861 ctttgtcacc gctcaatttt gtttcggtga tgcatgcatc aaaattgtca ggtatctttc
     4921 tgaattcctt atcctttgac atgcagaaaa gaaggtccat atatttttcc ggcaggtatt
     4981 tctttgcaca gacagctatt ttgtcacctt taagttcggg ttctccgtgc atcgggagcc
     5041 attttccttc tttttcatta ccgatgtaat taagttcgaa atcgacagct ttgccaagtt
     5101 tgtcaagagc cggtttgact cccgcaacaa cctgtgcgcc gtaaggacat ttactcataa
     5161 catggaaaga aagtttaact ttttccttgg gctcaggttc gcctgctttc ggacaggcag
     5221 taaacataaa tactgcggca acaacaattg ccaataagat cattttcttc aaagttacct
     5281 ccaaatggta taaattaacg aacaaaaaca aggccatcct ttcttttcgg gtctagattt
     5341 tacatttata agaaaaaaaa gcaattttta aagtgtccct taaaattcaa aaactatgcc
     5401 gaactcgaat ctgcttccgc tcagatcaaa acttttttcc tttccgaaat cgtttatttt
     5461 ttcaagacgg tagtggacaa acagcgatgt gtttgttatt cccgacagct cttcaagctt
     5521 tcttgaggcc gaatcgtcca tccagttcag agagatcatc agcccgaatg atccgtgaaa
     5581 accgaatgtt ccaccgtctt cagatgtttc gcctttttcc ctgatccacc agatatagta
     5641 atcaattccg atagttgcat aaggccacaa tggaaaatca tatccgggcc gaagtttcat
     5701 ttcacccatg agaggaataa aatgcatggc actttcatca gaacttttct ccccgtcagc
     5761 ttttaccgaa gtccctttga aacgggtgta tccgatcccc cctatgaaag accatctgat
     5821 ataattttca agcacaggca ctttaaaacc gaaagtaaga gaagggagat acctcaatcc
     5881 gtcaccccct atctccttga aagctccgtc tgcttcacta tctatttttg aaggatagta
     5941 aaaagagctg ttaaagctga attctccgtc aactcctttt aattcctgag gatctaaaac
     6001 aaatatccca gcaatgacca tcaggagaac aaccgctttt ctccttaaaa tgaaaaccgg
     6061 tatgagaaaa agccacatca gaagaatgat ctgcccgtaa gttgtcgtaa aaagccagcc
     6121 ggttatgaca aaaggaataa gaagcgttct tgtaactgct cttaaaaatt gactttctgc
     6181 aatgatccgc gcaggcattt tcccatattc atagtaagct tttatgaacg atctgcctgc
     6241 tgaaaacttt gcaaggacca agtccctgaa atcgcgcagt atcttaactt gaggatgaaa
     6301 atagcttccg aaaccggcag ttgcgacaaa acagaacccc ccgtcgtccc tgccgccctt
     6361 ttccttgtaa tttttccagt atccataggt agttacagcc gaacccgcag tttcaacata
     6421 tttttccgga tcggagtttc ctgcaatatc ttcggcatat attcttatcg tgtatacata
     6481 tttatcaaga ccgctgtcgt tgttgataag ttccagccct tccttaccgg aaagagaaaa
     6541 ctcccattcc tcgtcgtatg atgaactttc aacttctgtc gtaaacttca gctccgactg
     6601 cacctcagtc ccgtccctgt caaataatcc ggaaagagtt gcgtgatatt ttcctatttc
     6661 atccctttct tccttgtcat ccggatcaac aggaggcgtt acatagatga ttattcttct
     6721 gtctccacct tcaggattta ccgtatcatc atcattgatc tcgggagatt ggggagcagt
     6781 attgtcaaat gttattttca gggttttcgt cactttctca ggaattgtcg tggttgttac
     6841 cggtgctgtg tctgaatcat ctgtttcgtt atcttcattg tttccggaat tatcaagctc
     6901 aaccgtaagt tttatgtcgt aagttttatc agcccagctt ctatccgtgc ccgttccgga
     6961 atcatcatca ggcagctctt catcataagt ttcgtcttcg tcgtttacag tatcatcatc
     7021 gctctcttcg gcatcatcat cgatcagaac atcgtcatcg tctgtgtctg cggcggcagt
     7081 tatgtttctg aaatttacag aggtgcggag cgattcaaga agctcataga gttcagaacc
     7141 tttcaaagta agtgaattgt tgagatatcc gagttttgat ccgcttactt ccgttgaaga
     7201 gaaaatgtgt tcctcaactc cctgggtata tgaaagatag accttttcaa caagatcatc
     7261 tttgggaagg tcaaaaagga tctttatctc cccttcatag ttcaatgaac catgttccgg
     7321 ctccagacgg actttttcaa gatattccgc atcatccgct agaactgttg acaaaaaaaa
     7381 gatgatcgaa acaaaaactg taattttatt catccattcc cccgatcaaa gtttctgcaa
     7441 ttctcacagc attgtatgcc gccccttttc tcacattgtc agcgacgatc catgccgaaa
     7501 gtcttttccc gtcctctctc cctgtcctca aacggccgac gaaaacttca ttttttccgg
     7561 atgaatcgac aagtgtcgga tagatgagct gttcgaaatc gtccatgaca accatttcag
     7621 gatgttcgct gattttattt ctgacaagtt cgacatcgca ctttttttca agatcgacat
     7681 aaaccgtttc agcatgggag aaaaatgtcg gaactctaac tgtagtgaca tcgaagtcaa
     7741 ggtctggaat tgagagtatc ttccttgatt cattgatcat tttgacttcc tcttcacagt
     7801 atccgtcact gtggaaataa cctatctgcg gaatgagatt gaaagcaatt ctctgtggaa
     7861 atgctgcagg agccacgctt ctgcccgaaa aaagggctgt cacctgttct ctcagttcat
     7921 ctatcccctt tttccctgca ccgcttacgc tctgatatgt actcacaacc acttttttta
     7981 ttttaaaaag atcgttcacg actttaagaa aatagacaag ctgaatggtg gaacagttcg
     8041 gatttgctat tatttttgat ccatccggct taacgagatc tccatttact tcaggaacga
     8101 tcagcggaac actgtcattc attctgaacg ctgaactgtt atcaatgaca taaacccctt
     8161 tttgagcaaa caccggagca aactcaaggg caattgctga acccgcactg aaaagggcga
     8221 tgtcaatgtt ttttgactgt atagattcaa ccgtcatggg aatgacatca atttccttat
     8281 tgcggaactt cactgtttcg ccgatcgatc tttcgcttgc ataagggata agctcattta
     8341 cgggaaattt cctttcttca agtacactta tgaattcctg acccacaaga cctgtagcac
     8401 ccacaatggc aacattgaac tttttcatgt ttccctccaa aaaaaccgca gaaatatgca
     8461 ataaccgtac catcctgaga actcaacatt ttcaggatgt tacattatgt gaaaaaatga
     8521 caaaaaggca aattgacatt atgacacgcc ctgtctctcc aacggcgaac atttgcaaat
     8581 atctgaaact gctgtaaaat atcagcggtt aaaatcttgt tctcaaaaag gagatgtcat
     8641 gaagaaattt ttttctgtca ttcttattac aatctttttc gttgcatgca gcagcggtaa
     8701 aagctcaatt aatgatgaag atattgcaac aggcgaaaca aaagatgaaa atcagcagct
     8761 cccgacaagc gatgatgaac cgggagaagt tgaggatgac atgtccgacg acttgtccga
     8821 cgacatgtcc gacgaagcct tggcggagtc ggatgaagac gactcaccac ttcctgacga
     8881 agacaccgct ttctatgaag ataaatgcga ttgtttcgga tctgaatata cgattcctga
     8941 acagttcagg ggaattgacg gctggtgcaa acaggatgca gacagcgacg gacttcccaa
     9001 ctgcattgag gcagttgacg gagaattgac aaattctgat tcagatgctg atcccgatta
     9061 tcttgacaca gacagcgatg acgacggaat ccctgattct atcgagggtc tggaagatat
     9121 tgacaaagac ggcatcccca attacaggga tacagacagt gatggtgacg gatattccga
     9181 ttcagctgag tgtccgactc ttccctgccg tgacactgac ggagactcca ttcctgatta
     9241 tgttgatttt gattcggaca atgacgggct gaccgataaa gaggaaaaag atctcaatac
     9301 cgatccgctc aagcaggata gtgatgatga cggatttgat gatctttcag agatcgctta
     9361 cggatctgac cctcttgata aagctgacgg tatccccgaa gatgattttt atgttaaact
     9421 tccttttgaa gcaccggcag atgagatacg gatactgaaa tttaaaacca atgttgagaa
     9481 ggcggatgtt cttattcttt ttgatctttc tgattcaatg agtcaggaaa gcacgaatgt
     9541 aaaaaacggt atttcctctg aaattatcgg cggaataagt gcaaatataa ctgatgtcgc
     9601 attcggactc gcaacctttg atgactggaa gggactttca agatattcag atccgactga
     9661 aggaacaaat tattccggca caggcactct tcagcacacc gattcgatct tttcacttgt
     9721 tcagccagtt actaccgata tgtctgaaat aaccggttca ataaatggaa tacagaccgg
     9781 ctctctgtgc ggatgggaac ctcaccatga agcactttat caggttgcaa cgggggctgg
     9841 ctatactgga gatttctatt ttgaaacaac ttatgtcagc acaaacaaat gtgatgttgt
     9901 cggtaattat attcctgaaa ttcctgccgc aaactgtacc ggacatgaag gaaatatcgg
     9961 aggcggctgt ttcagagatg atgcaatgcc tataataatt atgctcagtg acgaggcatt
    10021 tactaatttc ggtgcacatt ttacatggaa tattccttat cacacaacag aagaggcgat
    10081 tgctgcactt aatgcaataa atgcaaaatt catcggaata gattcctgga gcgaagaaac
    10141 tttctggaca actgctccag aaaatgattt caaagcaata agcacagcaa ccggatcagt
    10201 tgatgcgaca accggcgatc cgtttttcta caagatcgga agcgacggaa caggactctc
    10261 agatcagatc gccgatgcag tttttgaact tacgacaaac ataaaacttg atgtctggac
    10321 tgcccgcgaa agtgttgcaa accctgaact tgtagacact tcgcttttca ttaaagcgct
    10381 cattcccgat tcatcagacc ctgtgaacgc ctatttttca aaagacacaa ccaccttcta
    10441 tcttgtaaat cccggtgcga aagtgaactt tgaaattaag ttccacaacg atttctatga
    10501 accggacaaa gccgaagcga ccgttttcag ggcaaaaata aatgttctcg gtgacggagc
    10561 ccttcttgac acaagggaag tcgtgatact tgttccggga atccattcaa gagataacta
    10621 aatatcaaaa taaacttctt ttctgtgacc gatcctgatg acaagtataa ccagttctgc
    10681 atcttttttc tcaaagataa ctctgtagtc cgcaactctg agacggtaaa tttttttatc
    10741 acccttcaac tgtttaaggt tgttttcaag tgaattaaaa tcatttgaaa ggatatcaag
    10801 tttgatcttt atcagtttct gaatgggaaa atcaatcttc tgcaacattt ttacagaagc
    10861 ctttgtaaaa agaattttgt atcgcatcag ttaatgttca gatccttgta aaccttttcc
    10921 gaatcaacca gttcctcttt gcctgactca acatctttta atattttttc agcaagatga
    10981 ctgtccatca tatcaaaata aattgaaagg gctgtttcaa tgatatgact ttttttcttt
    11041 gataaacctt ttgaaaatgt ttcaagttca gcaacaacat cttcgtccag tgtaaaagtt
    11101 gttttaattc tcgacatttt tccctccata cagacaaacg cacttacaat tgtatgtatg
    11161 attgtatgtt ttgtcaagtt gcgggttacc tccagccctg agttcatcga agggtcacat
    11221 ctttccattt ccctgaaaag agtaatgatt cttcttggtt atgatcacat ggtcaattat
    11281 ttcgatcccg attatctcac cggcttcctt gagtttacgg gtcatcagca agtcttcaac
    11341 ggatggttcc gggtttccgg aaggatggtt gtgggctatc acgatcgaag ccgccctgtc
    11401 agcgagtgcc ggtgcaaaaa cttctcttgg atgaaccatt gatttattga gtgtcccgat
    11461 gaaaatgacc cgcactttga tcacattgtt cgccccgtca agagttatca gaatgaaatg
    11521 ttcctgctgt tttgaagcat atccgtcaag aagatcggca acatcacgcg aaactttgat
    11581 gttcactttc tcagattcga catagaatct ttttgcaagc tcaaaagccg ccgcgatctg
    11641 acacgccttt gcatttccga caccttcaat gtttgtaagt ttttcaagag caattccgcc
    11701 aaccccctct ttttccagca tattttcaat atttcttgaa atcgtcatca ccggcatccc
    11761 tttctgtccg ctgccgagaa taagcgctat cagttctctg ttctttaaag ccgcagagcc
    11821 ctttgagagc atcttctccc gcggtttgtc atctgaataa agttcgtgta tctgcttcat
    11881 gaaaaaatcg ggctcctttt aaaaatgagc ttgaaacatt tcgcttagat taacaagaat
    11941 ttgttttatg tcagatggtc tgacaaaacc ataagatctt ttaaacactt ttctgtctaa
    12001 taaatttatt tttattttga acacttcccc aagtttcggg aattctcttt tttgcgacat
    12061 tgctctttgt ctccatcaaa atcgggctga aattcaagat cttcgatcaa cacatcatta
    12121 agaataacat cgggaacact ttcattgatt ccatcatcac ttttttcttt aaccattcct
    12181 ttcacaatat catccgcatt ttctaacggg acaataatcc agagaaaata gtcaacagga
    12241 ctttccattg agcggaaacc aacttccaaa caaggtttat ctaacagtat ctgatttcgc
    12301 tcaataacat tcattaaagc accttcaacg aaatgttcca tcgcttcttc gaatgatatg
    12361 ttcttaactt gctctcttgc aaaactgatt gaacagtcca acttgtcaaa ccaccagttc
    12421 tcgcaaatga aatgtgcttt tttcaacaaa gcttcctgtt cagaatgcga caacttgctc
    12481 gtatcataaa aaatatcgca cttcattgtt ttttcccttt ttttaaactt tctgaaaaat
    12541 ggtcttctat ttctattttt tcccacatat cagagtgtgc ctggaaaaga gaatgaatgg
    12601 aatcaagatt tattatcctg acttgtatgc cgttaatcct tgcgagtttt attgcaggaa
    12661 taagatcggt gtcattggtt atcagaacta ttctttcaac gagctttttg taagccattg
    12721 atgcaatgtc taagccgatt ttcatgtcaa cacccttttg aacataattt aaaggaccgc
    12781 ccttcggagt gtgatttacc aaccttccca tccgcaaagc aaaaaatggc tgactctcaa
    12841 ttttgttaaa gaaataatct gaattattat cgttatccgg ttttgcatca taaaaaaata
    12901 tcctgtaaag cacttcttca tcttttatta gtgagttaca gaaattcaga acttcttccg
    12961 gttttggata ataccgatga cctctttttc tattaagaat ttctcttaag tatccaccat
    13021 caatcagtat agaagtttta atgctcatta tgctaacctc caaaaaaatg gccgggattg
    13081 ctcccagcca ggaaatatgc ccgcctacgg gcgtagcgga tatataacag atcaaattaa
    13141 cgaatcataa agtactcata tttccatcca aatcaagttt ttttcatcat cgttcactac
    13201 aaaattgaac atccacaccc agattcattt ttgcttggaa tttcttcgtc gtcttcatcc
    13261 gactccgcca aggcttcgtc ggacaagtcg gtgtcgtcat cggtggcttc ttccgactcc
    13321 gccaaggctt cgtcggacaa gtcgtcggtg gcggcatcat cttcgtcagt cgtgagatcg
    13381 tcgtccgact ccgccaaggc ttcgtcggac aggtcgtcgg tggcctcatc atcgtctgtt
    13441 tcttcatccg actccgttga aacttcgtcg gacatgtctt cttcaacgat cgttacaccg
    13501 tcagatgttg tccatccact ttcataaccg cctgaattta ttacttttac attgagatat
    13561 attgtgtcgc cgatctcaac ttcaagattg tcgaaaacaa cctgatttgc ccctgtcacc
    13621 attgtttctt ttaaaactgt ttcatcctga tcggtgagtt tgacatgata ttttgcggca
    13681 cttggaactc cttcccataa tccgccgtaa ctttctccag tcaacatttc atcaatatcg
    13741 gtgtttgatg caatttctcc atcaattact gccacatctc tcacaaatgc cggggcggga
    13801 actgcaactt tctgcccgat cgcataaaga aatccatccc ccgctccgat taatatttcg
    13861 atctttccgt caccgttcac atcagcaagt atcggattcc cgattacccc gtcaaacttc
    13921 attgtccaga caagttctcc attaatatca acaacataca aattcccaag tttatcaccg
    13981 accacaaaat catcaccccc gttcccgtca atgtccccag tcacaatgtt tgaaagattt
    14041 gttccgccgc aaagcgttga agtggggatc acactcactg aaatatctgt aacagtcccg
    14101 ttattcaggc actttctcca gagcacactc ccgtcagcac cagaataagc agaaacatcc
    14161 ccgaatttcc ccccaagcac aatgtcaaaa ccttcatcac tgtcaatttt tgcaatgccg
    14221 gggtacaacg cagaatactg aaaaagcgaa tcctgatccc aacttatgtc aattgtccaa
    14281 agctcatttc cagaattgtc aatcgttctt ttaaatgatc cggttctatg atttccaaat
    14341 atttcagcag tttcatttcc gtttaagtcc gcagcaatcg aattgttcgg ccaggaagaa
    14401 aaagaaaact taagaattct tgtcatgttt ccatttataa attcagatgt gaaagaatgt
    14461 cccagaaaaa gatcatcaga tccatcatca tttatgtcac caagcagaac tgttgtattg
    14521 ttgtaatatc cggtaatgtc aacttgagga tcgtggcttc cgatctcgct tccgtcaatt
    14581 cccctcaaag cataaagcat cgccccttct tcttcagttg tgaatgtgaa agcgatgtct
    14641 tttaatccat ccccgtcaaa atcacccgca atgaaatttg caggatttgt cacaactttt
    14701 ccaacagtcc agccccaaag cagtcctcca tttgaagagt ggagatatat gtctgtgtag
    14761 ccctcactgt cagtaaaaag agaaatgaat tcgtgccttc cgttgccgtt actgtcaaaa
    14821 gcaaagagtg gctgagctga ctgattggtc tgagtccatt caacagttgg cggattttca
    14881 agatctgaat tttctgaatt aagaagaaca tgactgtttg ttgaaattgt tgaaagaatt
    14941 ttcccgccag ttgatgaatt tatgaattgt gaatcaccac tgaaagtccc tgtttctatt
    15001 accaattcat tgcttaatgt tgctccgcgg tcaaaaatgt ggatgaatcc atcattgcct
    15061 gtgagataaa ggtgaaaatc agttgaatca atgaatccat aactttttcc atcttttaaa
    15121 tatgccgtgt cactcacaac cggagttgat gcaacacctg aaaaagagat gagtttgtct
    15181 ccaactccat ctctgttaat gtcttcaatg agcaaaaccg catcatttga tccaactcta
    15241 acaattgcag gaacatcgcc tgcataaata actccgacat cttcgctgtc aactggcgtt
    15301 tttgtcacaa ctctcacttt gtccttggac cacaggtttt gaaaatcacc atcaaaacta
    15361 tatgcagaaa ttgttgaata aatccttaat ttttttgaag aatcgggagc actttttatt
    15421 ataaattctt tcactccatc cccgtttaag tctgcaactc cttcaagata tgtgttatca
    15481 agacgggctt gaaacaaccc gttgtctccg cgatagataa gagttgtcca gatgtcagga
    15541 gcgtttaccc catcaagatc ggttgccccc tcaagagtgt tattaaaaac tgaaacgaca
    15601 acatcaatca ccccgtcacc attgaaatcg gcaactgaac ccggaaccat cctgaaaccc
    15661 ttattcacag tgttgaaacc atattcatac tgccattgca cagcatcctc agcgaaatca
    15721 taaactgtga ttgaataaga ccccttgtca gattctgaag aagttctgcc ggtaaaaatg
    15781 aattcattct gatcgtcatt gtcaacattg atgatctgat taaacccgta agcataattt
    15841 ccatcaagaa ctctcgcttt gtcacgaatg agcgttccgt caatcgcctt aaaaacagaa
    15901 ataagaccgc cacttattct atctgttatg agttcttttt caccatcatt attcagatct
    15961 ccaaatgtga gctcaaatcc gtaaccttca atgttttcag ttatctgcca gatcatttgc
    16021 ggagttgtca caccacttga aaaatcataa gctcttacat cttttgtgtt ccatcttcct
    16081 ctgacaacca aatcaacaaa cccatcttca ttaacatcag caaactttgc aaaagttggt
    16141 gattctgtcg gaataattga atgaattgaa ccgttttctt tgtttattat ccggattccg
    16201 agattccctg caaccaccag ttcttttgtc ccgtcattgt ttagatcaat gatcttaaat
    16261 attcttgaag ctccgaaatt ttcactaacc cataacaaat tcaagttctt atcaaccgct
    16321 ttcaccaccc cattgatcga aaaaaagatg ttcccgtttt catcaactgc aacattattt
    16381 ggactcaaat tcccgccgcg gtaatatttc cataagattc ccggctcttt caccgccccg
    16441 ctcatctccg cacgaccggt cctgaacgag ttgttctgca cctcaggcca gaaaccgccc
    16501 gcaaaaagaa aaaccgttga aacagtcagc acagaaacga tcgaaaagat cttgattttc
    16561 cgcataattc aaaccctcca gtcctaaaat aacttcctaa aattataagg gagaaaggga
    16621 tttagtcaag aggggagtca gcccggggga aagcccagcc attctagcgt gggctaagat
    16681 aacgcggact ttcagcccgc aaatcagccg tctgaaggac ggtgtttgct tagcccgtgc
    16741 ccaacgggcc ggggagactt tcagcccgca aatcagccgt ctgatgacgg tgtttgctta
    16801 gccattcaca ccatcttgac cgccgtagct tcagcgaagg aggttccacg ggcgcgataa
    16861 atcgtgcccc tacacattca caccatcttg accgccatag ctttagcgaa ggtggttcac
    16921 ccgtcacccg tcacgcatca cgcatcacgc atcacccgtc acgcatcacg ggttttcccc
    16981 tacccatcgc ccaaatgttc ttttgcattt gttcatattt taaaaataat agtttgactt
    17041 tcacaatgaa tcaatgtaaa tgtgcaatcg ggcgcaacga tgggcttttt aaaaacaaac
    17101 ttgacattct ggctggttat gactattttg tttttatata gtgccctcga cagtaagctt
    17161 cattcgacat tggatgacag ccgagctcga gggctttttt tttgaaaagg ggaatattaa
    17221 atgaaagaag cagcaattct ggttgacggt gcgttctttt taaaaagata ttttgcgatc
    17281 gtttcaaaag ttcatccgcc aaatgtaatt gctgacaatc tttatgcgac acttctcaat
    17341 catctttatt cttttcgcga cattgaaggt acttattctt tgtacagaat attcttttat
    17401 gactgtcctc cgcttgaaaa gaaagttcga caccctctaa ctggcagaaa cattgattac
    17461 agcaaaacag agcttgtatc tttcagaaaa gaacttcata atgaactgaa aaagaaaaga
    17521 aaactggcat tacggctggg acacttaaaa gatgaaagct gggttattga aaaaaacaga
    17581 ctgaaaaaac ttttaaaaaa agagatcacg attgatgatt tgaccgaaga agacattctc
    17641 cacagcatca agcaaaaaag catcgatatg aaaatcggaa tagatattgc atctctggca
    17701 tacaaaaagc tcgttaaaaa aatcattctt gtagcaggag acagcgactt tgttccagca
    17761 gcaaagttag cacgaagaga aggagttgat tttgtactag atcccatgtg gaaccacatt
    17821 gccgaagact taaatgaaca cattgacggg ctaaagtcat attttcccaa ccctttaaaa
    17881 cgacaaggat tcttccccaa atcataagcc accactcgct tttgctcgat caccgggtct
    17941 ttatcattaa aaca
//
LOCUS       DAOOYB010000024        17952 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_3024_length_17952_cov_9.729899, whole genome shotgun sequence.
ACCESSION   DAOOYB010000024 DAOOYB010000000
VERSION     DAOOYB010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 17952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17952
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3024_length_17952_cov_9.729899"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1645
                     /locus_tag="PL056_02505"
     CDS             <1..1645
                     /locus_tag="PL056_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45920.1"
                     /translation="FVNEKTEVVCVCNPGHYGLNCEYKNLDYSDKFPVGDEYFLPEPN
                     CKKDEDCSEGMKCSETGFCYCDGKIEFTDSVLRNEIISATGKISFIGEDLLNITKLTL
                     SQVSTINNMRCLPNLRALQIFQPNSELKFEELSNLENLQYLVIFDYNGISDFSPILKL
                     KKLLFLNLELTEDNYKFLEKMNPDNQIEGIYFSFFKGDYPENFNFLDNFRKLTSAAIT
                     FGQNTPKNKDCLIDMSNITQNNEISHLRIYAGNCEIKEIQQLEKLTNLKNLTIGHMML
                     VDGTGKRHLQLSSFPVLKNVTQLYIDSTVFDAKVIDKFPSITVLYIWQEGLPVEKLDF
                     IEGLVNLSTFAFSDDIKIVSQFNNEKYDWINKMNNLRYLSIDPYKSRNYDFIKGLKHI
                     EEMGILSQSGWEQKYTEDYEINGSVGDDYSENLLYLMGLADRTYHLDNVKSFRKNCDK
                     GGAVCNKKFREYFYPEASLGYGFSLCPNPIDKDDEDVKYLVEKGVGLTIGNNSQNCEA
                     LFSDGENITRMNHPLLNRKQKLDFRMDITDNSGIKWISK"
     gene            complement(1671..2309)
                     /gene="lexA"
                     /locus_tag="PL056_02510"
     CDS             complement(1671..2309)
                     /gene="lexA"
                     /locus_tag="PL056_02510"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HPM45921.1"
                     /translation="MKNFSNEDLTVSQRRVLEAIRSHIEKNGFPPTSREIGKELGITA
                     TSVFEQLDRLEKKGFIKRQKKAARSIEILDQRKDDNLTEEKVRLIKIPVLGTIAAGQP
                     ILADENFESEILVDESSIGKGKFFALKVKGDSMINAGINQGDLVIIRRQPVAENGEIV
                     AALIDNEATIKRLKMDNGKVLLMPENEKYSPIEVTYREDFRILGKLITTVSY"
     gene            2423..2499
                     /locus_tag="PL056_02515"
     tRNA            2423..2499
                     /locus_tag="PL056_02515"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2457..2459,aa:Pro,seq:cgg)
     gene            2655..3191
                     /locus_tag="PL056_02520"
     CDS             2655..3191
                     /locus_tag="PL056_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45922.1"
                     /translation="MIARLDNRTAQRCSDPFVIDISEIREDRPIVREIKGIKFFLGTV
                     IPEKKEDDAIVDKEDHGVPLKEDTGENQVQQQVIESIEDSDIAVYLVKDDGSFEVSSD
                     EVEDAVVNIQASVKKVDIIITSAIPAAKEKIIKSSTQLVNITKDTDSAVFTYSVIRQD
                     SGEKVVSLLSRDDFGTLF"
     gene            3261..5615
                     /locus_tag="PL056_02525"
     CDS             3261..5615
                     /locus_tag="PL056_02525"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HPM45923.1"
                     /translation="MKDSNGRSPFLKFIESYQLKINKWLREDRIDHALTIFFVFSLLI
                     AYMTIPESMKINYPTSIKDVGSVLKYSVRASRDYIITNEEKTKQNRLLAEKDVPLFYV
                     ASESENASKTIKNAFGHMRESAKEHLEKTISSMNTEIPDEYIKLAASSVPSLPKNPGE
                     AAFIRNELFSHFERERDAFHKILGFPVSSEIFTILSSNFFSSFIEKSIEDIMSRLNTY
                     YVSREGIPADYGLPHVSIKKGEAVINVPVAKIITGSTLREEIRHTQLILQHDKEFTET
                     GINMMAELVFWVLKDNISYSSSLTEESKLVAWNNAPKEEFKIKNGEIIKRAGEVLTKR
                     DIMVFQEMAKQTSQRSLPLIYLQNLIFIMIFSVVLLFSFKKTVSKFSSRNKDILLICI
                     QTIFSIIIFEFIVSLSIPFSNWMGSIDARIFYFLMPVPFVIATVRLLVNSETAICFLI
                     FTTALFILTFPDNFYFPVFYLAGSLFYMFLVTHIEKRGNIIKVSFFLSLFLMMMTVLI
                     FLMDQTLPFDNLPRALLLTFSGAMLSGMLIMAIIPVYEWLFGYTTEITFLEYSTLNHP
                     LMKRMAVHANGTYQHSLTLGSIVEVAAREIGLSSLACKVMAYFHDIGKLERPEYFTEN
                     QAGKNIHDELPSHSMSAMVIINHVKQGLELAKKYRLGEKIEDAVSQHHGTTLVEYFYK
                     QAKKGDKNVSESTYRYPGPKPASREAALLMVADSCEAAVRSIPEKNYQKISDTVVKII
                     AGKLADGQLSECNMTLKDLKKIEMSIIKTLSGIYHARIEYPDQK"
     gene            5628..6092
                     /gene="ybeY"
                     /locus_tag="PL056_02530"
     CDS             5628..6092
                     /gene="ybeY"
                     /locus_tag="PL056_02530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HPM45924.1"
                     /translation="MFLSIQHKNGFNINRAELRNAVKKTLVHNGMKISDPYEISIVFT
                     GRNEIKEMNREYRNIDRATDVISFAFSEGEGSQFVPFLLGDIFICPEVVADHAEKFGT
                     TFENELIFVVVHGVLHLLGFDHNRKSDREKMREAEDIVMKELVGNWNGRCEK"
     gene            6073..7722
                     /gene="lnt"
                     /locus_tag="PL056_02535"
     CDS             6073..7722
                     /gene="lnt"
                     /locus_tag="PL056_02535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HPM45925.1"
                     /translation="MDAVKSKIIKLAVASILFIVCFALSFPFVIPGDPQPVFIHRFIG
                     WIFIFPVMPLFLYVIESFEKLKHKIGMLYLICIPANGLVFYWAYYSIHVYGGVPKSYT
                     ILLVFVMFTALSTFWLFFLLIYEFLKKDGRIAPWKVAASWVTAETVRTFFPVDFYWSC
                     FGHSQYNNKLTLQWASIGAIYFLSFMIVWISVYIFARLRGEKNWKEGIILIVSVTILI
                     TYSVVRIESFRKLEPFKEVKVAIMQPAINQFDINSKEKNLEDIIAVFTSQINSFDKDT
                     DLLVWHEAAIPVRVPVGFDNFRYLWTKYFPDAYRFENQIVGLDMIDRDKREFYNSAGF
                     VKGDRIEKIYRKIKLAPFGEYLPMSDFLHSIGLSTIVPNSVGTFMRGTEHTVFDFGKF
                     NASILICYDGTFSENVRDFVRNGAELLVNISNDAWFGFSSEITQHGSFYPFRAVESGR
                     TIIRASNVGISEVILPDGTRENQTNLFERTSINRKVPLYQYDTIYLKYGNWFLYLVLI
                     ITFGMIFLRFSEIRSAKVQHVEQEPEIKKKRAGRNKNKTKK"
     gene            7823..8044
                     /locus_tag="PL056_02540"
     CDS             7823..8044
                     /locus_tag="PL056_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45926.1"
                     /translation="MRVGAFAAKKGGYTGNTDVQPGFFTDYNGKPSIPVATGGEVYLI
                     DDVNAGTLHIYETVNAFIENTMCKTYDEK"
     gene            8141..8536
                     /locus_tag="PL056_02545"
     CDS             8141..8536
                     /locus_tag="PL056_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016662801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HPM45927.1"
                     /translation="MKGKAEIIEYWKELAEYDLETAKAMLKTKRYLYVGFMCHQVIEK
                     IFKGYFVSRKNETPQYTHNLLKLAKAGDFYGEFSDRQKDLIDYLEPLNIEARYPSYKE
                     ALLKELTKDRIEKILNETEELYEWIKEKF"
     gene            8515..8823
                     /locus_tag="PL056_02550"
     CDS             8515..8823
                     /locus_tag="PL056_02550"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HPM45928.1"
                     /translation="MDKREVLEKLRRFSEIVVEKYNPLKIVLFGSYAKGTNKEESDID
                     VAVIVEKIDGSFLEKEAGLYKIRRNIDENIEPVLFEAGSDKSGFLESILSYGEVIYQR
                     "
     gene            complement(8846..9667)
                     /locus_tag="PL056_02555"
     CDS             complement(8846..9667)
                     /locus_tag="PL056_02555"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPM45929.1"
                     /translation="MSRRIYQLIFFIIQNPFISNFMTGRPYNGPAKSFCAPGLHCYSC
                     PSMAFGCPLGTIQYVIKYAAMIPYYTLGTLSAIGAVFGSASCGYVCPFGLFQELLHSI
                     SPWKSDTAKLPQPFRYIKWGNLILLVIIVPAFGLAQGFCAWICPSGLIMAGLPIIAAN
                     EGIRASIGITFYWKLILTTVFIIFIMREKRFFCRYICPLGLILGFFNKISLYQIKLDK
                     NKCVTCRACQKSCPMGLKLPEEINSIDCIKCGECVPSCPSKFGALSKGFEIKKIL"
     gene            complement(9660..9872)
                     /locus_tag="PL056_02560"
     CDS             complement(9660..9872)
                     /locus_tag="PL056_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45930.1"
                     /translation="MKKIVIVSIILIISLIFLIKGWVHEIDEDGTKEIDLVNAAGKNV
                     CMSCIGLADENIFEKLFGTSEKKDNE"
     gene            complement(9869..10648)
                     /locus_tag="PL056_02565"
     CDS             complement(9869..10648)
                     /locus_tag="PL056_02565"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HPM45931.1"
                     /translation="MKLFCGIDIGSRTGKIVIINDIKEIVFSSTIETIAGATNTINKL
                     SNSIPDEIKKNIVSKAVTGYGRESVFDPSTFTLTEITAHYLGVISLHPETRTVIDIGG
                     QDSKVITIDNDSRIKDFVMNDRCAAGTGRFLEVMCGRIGFDLEKFALLDIEGIESQKI
                     NSTCTVFAESEIISMISRDVPDIVIASTLAEMVARNTYNMARRTHPSDPFFMSGGVSK
                     IKPVKFHLQKLFKSDILTSGFSQIMGALGAAIFAMNKTDKK"
     gene            complement(10645..11439)
                     /gene="mazG"
                     /locus_tag="PL056_02570"
     CDS             complement(10645..11439)
                     /gene="mazG"
                     /locus_tag="PL056_02570"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007007456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HPM45932.1"
                     /translation="MSEMKGITKLLEVMAVLRGENGCPWDKEQSIQSLRPYIIEEAFE
                     TVEALDEFDRSIPESVIELKKELGDLLLQVVFIAQMAGEENIFDFNDIAETLTHKLIS
                     RHPHVFGEDHCRNSAEVLTNWNLIKRKKEERKHLLDGIPRSMPAVLLAQRYASRAASV
                     GFDWEKWEDVTDKIEEEQAELLEAVKENDAAHIEHELGDLLFAVINLGRKLGVDNEKA
                     LKMCAERFKARFDKMEDLDPSFIEGGRPVKELEELWQKAKKELKKD"
     gene            complement(11441..12877)
                     /gene="purF"
                     /locus_tag="PL056_02575"
     CDS             complement(11441..12877)
                     /gene="purF"
                     /locus_tag="PL056_02575"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HPM45933.1"
                     /translation="MCGIAGVFNVENGEILVAEILFAIQHRGQESCGIASKNEFGNIS
                     GYRGMGLVKNVLTESILAKYQGKIAIGHVRYPTAGRSDETNSQPHVIELPEGPHLAIC
                     SNGDIINYYELRTWLEKEYEFTFKSDNDGELIGRLIAFNLIHRKMTIENAIVETQSQL
                     KGAFSSIVIYRDQMFAFRDPHAYRPYILGHLQGVSETGEYSSNGVVLASESCAFGIVG
                     AKMIRELEPGEIIKLEKDKPMKTIAVNSKNKQHCVFELIYFSRPDTIVYEEKVYAVRK
                     KIGARLAEYDKELGFGEDTVVMAVPDSSNFVALGYAHGSGLQFDMGLLRNHYVGRTFT
                     KPDQKQRDEGVRHKFNPLPEFFKGKKVVLVDDSIVRGTTLRKIVRMIRSAGAKEVHVR
                     IGSPRVIGSCYYGIDTPTRKELIAAIKNNDEICQFLEAETLRYIEVTDFEKILKNSDN
                     FCFACFNLKYEYKPEDFHQKVNSPDKRC"
     gene            13032..13928
                     /locus_tag="PL056_02580"
     CDS             13032..13928
                     /locus_tag="PL056_02580"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HPM45934.1"
                     /translation="MITECPSCKKVISVNVPGTVMCPKCNSLVFIGDPLKDEENRVIK
                     TARELKLERDVESSKKKIEDENGFFNPFGEMKKVKKAGFFKGTPWDRWHELGFAEAFI
                     KTTKEIATSPEKFFKDMKHAANPGLMPIYGIILAFLTVLFQTFWVLKLFQSYFPDFAT
                     FRDTFLKMGDLGASIFADENKLRMIYDQMYPDGGSLFSHLVLTPFMSIVVTAFILHLG
                     SVMLGSKTRLTHFYRMSSFIMVTGLFSIFPVIGNIAGFFWRVFLVHKGGMALNNFNGR
                     KAYIFTGFYVAMQLFFSTVGII"
     gene            13925..14404
                     /gene="ispF"
                     /locus_tag="PL056_02585"
     CDS             13925..14404
                     /gene="ispF"
                     /locus_tag="PL056_02585"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019917339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HPM45935.1"
                     /translation="MIFRVGQGFDVHRFEEGRELYLGGVKIDHTGLAGHSDADVLIHA
                     VIDALLGASALGDIGELFPDKDPEFKNIRSTLLLENVVRKLNDNNWLIENIDVTVICE
                     TPKISLYRELIREQISKITGIGTDRIMVKGKTTEKLGFTGRGEGIVAMAVTLLKKEE"
     gene            14405..15007
                     /locus_tag="PL056_02590"
     CDS             14405..15007
                     /locus_tag="PL056_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45936.1"
                     /translation="MKKISFYIGRKNTVTFFLASAVLLFGIYSFFGEVVYFFSGSEAI
                     DLKDAMDIDKANFEKVDDGDFVQVKGITSVQGGAIQRGITGTEYILYYLTGSSKFIIN
                     EKNDGESKGPQYKTVRGRAYKFKTNSNAARMRNFFARSFMIEMDEDGILIEAGLEPGS
                     DYAALIFFIMLIAAMGLNIYLFIRPLKQKEDPMENDIDEI"
     gene            14997..16460
                     /gene="gltX"
                     /locus_tag="PL056_02595"
     CDS             14997..16460
                     /gene="gltX"
                     /locus_tag="PL056_02595"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HPM45937.1"
                     /translation="MKFEKVRTRFAPSPTGYMHVGNLRTALYAWLIARKNNGTFILRI
                     EDTDQERKIEGAVDIIYRTLAETGLNHDEGPDVGGDCGPYVQSERMSSGIYEKCALEL
                     IEKGHAYRCFCTKERLDALRAECEAKGTQTRYDRHCLDLGEEEIKKNLENKVPFVVRQ
                     KMPLTGTTSFHDEVYGDITIENSTMDDLILMKSDGYPTYNFANVVDDHLMGITHVVRG
                     SEYLISTPKYNLLYEAFGWEKPFYVHVSPVMRDAQRKLSKRDGDASYADFIEKGCLKE
                     ALLNYVALLGWNPGTDQEKFTIPELINAFDISGISKSPAIFDEAKLRWLNSEYIRAMS
                     HEEFLQHAMPFIKKTVKRDDVNFDVIAEGLHKRTEYFSDIPPQLDFIDELPEYIAELY
                     EHKKMKTDKVNSLQALEIMLPALESFSDWDHEKLYLMLNDLAEKSGINKKIILWSLRV
                     ALSGKELTPGGGTDIAAIIGKEESIKRLKKGIELLKV"
     gene            16483..17469
                     /locus_tag="PL056_02600"
     CDS             16483..17469
                     /locus_tag="PL056_02600"
                     /inference="COORDINATES: protein motif:HMM:NF005741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HPM45938.1"
                     /translation="MTDIKTNYLGLELTSPIVAGSCSITSSVENLKKLEVAGAGAVVL
                     KSLFEEQIVEERIKSATQGIYSFFHPESMEYISQMSMNFGPEYYLDLIKNAKKEISIP
                     VIASINCVSKEYWVEFAKKIESAGADAIELNISHVTHDIEKSADSVTDDYLATFKAVK
                     EGVRIPVSVKMGQNFTNIARMVKLLETHGASGVVLFNRFYQVDIDIDKLELVPGYVRS
                     SRKDITETIRWISIISGKTKLSLSGSRGVSDGRDVIKMILAGADTVQTVSALYQNGID
                     SIAKMNLEILDWMERKNFKSLSEFKGLLSQKKIDNPDAWERQQYIKALVGIE"
     gene            17585..17944
                     /locus_tag="PL056_02605"
     CDS             17585..17944
                     /locus_tag="PL056_02605"
                     /inference="COORDINATES: protein motif:HMM:NF013665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HPM45939.1"
                     /translation="MDFVKMVHEKAVKAGKTIVLPEGCEPRTVKAATIITKDKLAKVI
                     LVGNEADILKTAQKENVSLDGIKIVEPLKSEWYQDFVSTFYEMRKNKGVDMGRAAEIM
                     KDEPKSFDSDLNSRKMV"
BASE COUNT         5544 a         3353 c         3803 g         5252 t
ORIGIN      
        1 gtttgtcaac gaaaaaacag aggtagtttg tgtatgtaac cctggacact atggacttaa
       61 ttgtgaatat aaaaatctgg attattctga taaatttcct gtaggtgacg aatattttct
      121 tcctgaaccg aattgcaaaa aagacgaaga ttgcagtgaa gggatgaagt gcagcgaaac
      181 aggattttgt tattgtgatg gcaaaatcga attcacagat tctgttttaa gaaatgaaat
      241 aatatctgca acaggcaaaa tttcttttat aggagaagat ctcctaaata ttacaaaact
      301 tactttaagt caggtctcaa cgatcaacaa catgagatgt ttgcccaatt taagggcatt
      361 gcaaattttc caaccaaatt ctgaactaaa atttgaagaa ttatcaaatc ttgaaaatct
      421 ccagtatctt gtaatttttg attataacgg catatctgat ttttctccca tcctgaaatt
      481 aaagaaactt ctttttctta atttagaatt aacggaagat aattataagt ttttagagaa
      541 aatgaatccc gataatcaga tagaaggaat atattttagt ttttttaaag gtgattatcc
      601 ggagaacttt aattttcttg ataattttag gaaactcaca tctgcagcaa taacgtttgg
      661 tcaaaatact ccaaaaaaca aggattgttt aattgatatg tcaaatatta cccaaaataa
      721 tgaaatatca catcttagaa tatatgcagg aaattgtgaa ataaaggaaa ttcagcagct
      781 tgaaaaatta actaatctta agaatctaac tattggacat atgatgctcg ttgacggaac
      841 aggtaagaga catttgcagt tgtcttcttt tcctgtttta aaaaatgtta ctcagcttta
      901 tatagacagc actgtttttg atgcaaaagt tattgacaaa tttccttcaa ttacagtcct
      961 ttatatttgg caagagggat tgcctgttga aaagttggat tttatcgagg ggttggtaaa
     1021 tctttcaact tttgcatttt ctgatgatat taaaattgta agtcagttta acaatgaaaa
     1081 atatgattgg atcaataaaa tgaataattt gagatattta tccattgatc cttataaaag
     1141 cagaaactat gattttatta aaggattaaa gcatattgaa gaaatgggga ttctttctca
     1201 atcaggttgg gaacaaaaat atactgaaga ttatgaaatt aatggatctg tcggagatga
     1261 ttattccgaa aatcttttgt atttgatggg attagctgat agaacatatc atttggacaa
     1321 tgtaaaatca ttcagaaaga attgtgataa aggaggtgct gtttgtaaca aaaaattcag
     1381 agaatatttt tatcctgaag cgagtttagg atatggtttt tctctttgtc ctaatccaat
     1441 agacaaagat gatgaagatg ttaaatattt agttgagaaa ggagtcggac tgacaatagg
     1501 aaataacagt caaaactgtg aggctctatt ttctgatgga gaaaatataa cgagaatgaa
     1561 tcatccactt cttaatagaa aacaaaaact ggatttcaga atggatataa ccgacaactc
     1621 aggaataaaa tggatttcaa aatagaaaac attgaaaagc attaatcttt tcaataactc
     1681 acagtcgtta tcagctttcc taatatcctg aagtcttcgc ggtaggtcac ttctatcggg
     1741 gaatattttt cattttctgg catgaggaga actttgccgt tatccatttt aagccgtttt
     1801 atggttgctt cgttatcaat gagagcggca acgatctcgc cgttctctgc aacgggctgt
     1861 ctgcggatga ttacaagatc accctgattg ataccggcgt ttatcatgct gtcgcccttg
     1921 actttgaggg caaagaactt ccctttgccg atgctgcttt cgtcaacgag tatttcactt
     1981 tcaaaatttt catcggcgag tatcggctgt cctgcggcta ttgttccaag cacaggtatt
     2041 ttgataagtc gaaccttctc ttctgtaagg ttgtcatctt tgcgctgatc aagtatttca
     2101 attgatcttg ccgctttttt ctgccgtttt atgaaacctt tcttctcaag acggtcaagt
     2161 tgctcaaaga cacttgttgc agtaattcca agctctttgc ctatttctct tgatgtcgga
     2221 gggaaaccgt tcttttcaat gtgacttcta attgcttcaa gaaccctacg ctgtgaaaca
     2281 gtaaggtctt cgtttgaaaa atttttcata atatcacctc aaaaataata agaacttgct
     2341 gatcatatac ctgaaaaatg taagggtcaa gaaaaaagtt tgaaatttta taagtaagtg
     2401 gtataaagca agccgtttta gtcgggatgt agctcagcct ggtagagtac tacgttcggg
     2461 acgtaggagt cgctggttcg aacccagtca tcccgaccat tttggttcct gatgtgaaaa
     2521 aaacgcttct aatcttattc ctgttaataa tttcactttc aatatcagtg gtttacatat
     2581 ttaaagatta tggtatcacc catggatgtg tcattattac ttcaggattt aacaacccgt
     2641 cccggctgga ttcaatgata gcccgtcttg ataacagaac tgctcaaagg tgttcggatc
     2701 cttttgtaat tgatatatca gaaattagag aagacaggcc gatcgtaagg gaaattaaag
     2761 ggataaaatt ctttctgggc actgtgatac ctgaaaaaaa ggaagatgac gcaattgttg
     2821 acaaagagga tcacggcgtt cctttgaaag aggatacagg tgagaatcag gtgcagcaac
     2881 aagtcataga aagcattgag gatagtgaca tagcagttta tcttgtaaaa gatgacggtt
     2941 catttgaagt ttcctctgac gaagttgaag atgccgtagt taatatccag gcatctgtaa
     3001 aaaaggtcga tattataata acttcagcca ttcctgccgc aaaagaaaag atcattaaaa
     3061 gcagtacgca acttgttaat attacgaaag atactgactc cgcggtcttt acatattctg
     3121 tcatacggca ggatagcggt gaaaaagttg tctcacttct ttccagagat gatttcggaa
     3181 cacttttctg acaattttga cttgagtatt tttttagtcc ataataccgc ccggatgttg
     3241 ataattttct caggggatga atgaaagatt caaacggcag atcacccttt ttaaaattta
     3301 ttgagtcata tcagctcaag ataaataaat ggctcaggga agacaggata gatcacgccc
     3361 tcacaatatt cttcgttttt tctctgctta ttgcatacat gacaatccct gaatcgatga
     3421 aaataaatta tcccacttcc ataaaggatg tcggaagtgt tctgaaatat tcggtaaggg
     3481 ccagcaggga ttacataata accaacgagg agaagactaa gcagaaccgg ctgctggctg
     3541 aaaaggatgt ccctttattt tatgtcgcat ctgaatctga aaatgcatca aagaccatta
     3601 agaatgcatt cgggcacatg agggagagcg caaaagagca ccttgaaaaa acgatttctt
     3661 caatgaatac agaaattcct gatgaatata taaaacttgc ggcaagttcc gttccctctc
     3721 ttcccaagaa tccaggagaa gccgcattca taagaaatga acttttcagc cactttgaaa
     3781 gagaaaggga tgcgtttcat aaaatactcg gtttccctgt cagcagtgag atattcacca
     3841 tcctctcgag caattttttc tcttctttca ttgaaaagtc tatcgaagac ataatgtcaa
     3901 gacttaatac atattatgtt tcaagagagg gtatccctgc tgattatggg ttgccgcatg
     3961 tttcaataaa aaaaggagaa gcagttataa atgtcccggt tgcaaagatc ataaccggat
     4021 caacactgcg cgaagagatc agacacactc agcttatcct ccagcatgat aaggaattta
     4081 ctgaaacagg gataaacatg atggcggaac tggtcttctg ggtgctgaag gataatattt
     4141 cctacagctc ttcattgaca gaagagagca agctcgtcgc atggaataat gctccgaaag
     4201 aggaattcaa gataaaaaac ggcgaaataa tcaaaagggc aggcgaggtt cttacaaaaa
     4261 gggacatcat ggtgttccag gaaatggcaa aacagacttc acaaagatca ctgcctttga
     4321 tatatctgca gaaccttatt tttattatga tattcagcgt ggtactcctt ttttcattta
     4381 aaaagactgt cagcaagttc agttcaagga ataaggatat tcttctgata tgcatccaga
     4441 cgatcttttc aataataatc tttgaattta ttgtcagtct ttcaatccct ttcagcaact
     4501 ggatgggcag cattgatgca agaatattct attttctgat gcctgttccg tttgtaatcg
     4561 caacggtacg gcttcttgtg aacagcgaga ctgctatttg tttcctaata tttaccacag
     4621 cactttttat tctcacattc cctgacaact tctattttcc ggtgttctat ctcgcgggat
     4681 cattattcta catgtttctt gtgacccaca ttgagaaaag gggaaacata atcaaggtga
     4741 gtttttttct ttcacttttc cttatgatga tgacggtgct gatatttctt atggatcaga
     4801 cactgccttt tgacaatctt cccagagcac ttcttcttac attttcagga gcaatgcttt
     4861 caggaatgct tattatggca atcataccgg tctatgaatg gcttttcggg tatacgacgg
     4921 agatcacatt tcttgaatat tccacactta accatccgct gatgaaaaga atggctgttc
     4981 acgccaacgg tacatatcag cacagtctga cgctcggttc aattgttgaa gttgccgcaa
     5041 gagagatcgg tctaagctca ctcgcctgca aggttatggc atattttcat gatattggta
     5101 aactggaaag acccgaatat tttactgaaa atcaggcagg gaagaacatt cacgatgagc
     5161 tcccgtcaca cagtatgtct gcaatggtaa taatcaatca tgttaaacag ggacttgagc
     5221 tggcgaaaaa ataccggctg ggggaaaaga tagaagatgc agtcagccag catcacggaa
     5281 ctacgctggt tgaatatttt tacaagcagg caaaaaaagg ggacaaaaat gtaagcgagt
     5341 caacttacag atatcccggt ccaaagcccg catcaaggga agcggctctt cttatggttg
     5401 ctgacagttg cgaggcggca gtgcgaagca ttcctgaaaa gaattatcag aaaatatcag
     5461 atacagtggt caaaattatt gcaggcaaac ttgctgacgg acagctttcc gaatgtaata
     5521 tgacccttaa agatctgaaa aagatagaga tgagcatcat aaaaacgctc tccggaatat
     5581 atcacgcaag aatcgaatat cctgatcaga aataagcaga tatgaagatg ttcctgtcaa
     5641 ttcagcataa aaacggattc aatataaaca gggcggaatt aagaaatgct gtaaagaaaa
     5701 cactcgtcca taacggcatg aaaatatcag atccttacga gatctcaata gtttttacag
     5761 gcaggaatga gatcaaggaa atgaacaggg aatacagaaa tatagatagg gcgacagatg
     5821 tcatatcatt cgcattttca gaaggtgaag gttctcagtt tgttcctttt cttctgggtg
     5881 atattttcat ctgtcctgaa gtcgtcgcag atcatgcaga aaaatttgga acgacatttg
     5941 aaaacgaact gatctttgtt gttgttcacg gagttctcca tcttttgggt tttgaccaca
     6001 acagaaaaag cgaccgtgaa aaaatgcgtg aagcggaaga tattgtaatg aaagaacttg
     6061 taggaaactg gaatggacgc tgtgaaaagt aagataatta aattagctgt cgcatcgata
     6121 ctctttattg tgtgctttgc actgagtttc ccttttgtca taccgggaga tcctcagccg
     6181 gttttcattc accgttttat cggatggata tttattttcc ctgtcatgcc tctattttta
     6241 tatgtcattg aatcttttga gaaattaaag cacaaaatag ggatgctcta tctcatttgc
     6301 attcctgcga atggactggt cttttactgg gcttattatt ccattcatgt ttacggcgga
     6361 gttcctaagt catatacgat ccttcttgtt tttgtaatgt tcacagccct ttccactttc
     6421 tggctctttt ttctgcttat ttacgaattt ctcaagaaag acggtcgcat agctccatgg
     6481 aaagttgcgg cttcgtgggt tacggcagaa acggtgagaa ctttctttcc ggtcgatttc
     6541 tactggtcat gtttcggtca ttcacagtac aacaacaagc tcacactgca atgggcatct
     6601 atcggagcga tatattttct ttcgttcatg atcgtctgga tatcggttta tatttttgcc
     6661 aggttgagag gggaaaagaa ctggaaagaa gggataatac tcattgtttc tgtaacaatt
     6721 ctgataactt attccgttgt aagaattgaa agtttcagga aacttgaacc gttcaaagag
     6781 gtcaaggtcg caataatgca gcccgccata aatcagtttg atataaactc aaaggaaaaa
     6841 aaccttgagg atatcatcgc ggtttttacg agtcagataa acagctttga caaagatacc
     6901 gatcttcttg tctggcatga agccgcaata cctgtcagag ttccggtcgg atttgataac
     6961 ttcagatatc tgtggacaaa atattttcca gatgcataca gatttgaaaa tcagatagtc
     7021 ggtcttgaca tgatcgaccg cgacaaaagg gagttttata attcagcagg ttttgttaaa
     7081 ggggaccgga tcgaaaaaat ttacaggaaa ataaagcttg caccgtttgg agagtatctt
     7141 ccgatgagcg attttcttca cagcatcggt ctttcgacga tcgttccaaa cagtgtcgga
     7201 actttcatga gaggaacgga acatacggtt tttgatttcg gaaagttcaa tgcatcaatt
     7261 ttgatctgtt atgacggaac attcagtgaa aatgtgagag attttgtcag gaacggtgcg
     7321 gaacttcttg taaatatttc aaacgatgcg tggttcggtt tttcaagtga gatcactcag
     7381 catggaagtt tttatccatt cagagcagtt gagtctggca gaacgataat ccgtgcaagc
     7441 aatgtcggaa taagtgaagt gatactcccc gacggcacaa gagaaaatca gacaaatctt
     7501 ttcgaacgca cttcaattaa tagaaaagtg ccgttatatc aatatgatac gatctatctt
     7561 aaatacggga actggttttt gtaccttgtg ctcattatca cattcggtat gatatttctg
     7621 cggttttcgg agatcagatc agcaaaagtt caacatgttg aacaggaacc ggaaattaaa
     7681 aagaaacgag cggggagaaa taaaaataaa acaaaaaagt gaggtttcat gatgttttta
     7741 tgaaagtccc gcaaaatttt caagcaatat agcagttcag cacacttatg atgaaacggt
     7801 cacactcctt gttgacggta aaatgagagt gggggcattt gccgcaaaaa aagggggata
     7861 cacaggcaac acagatgttc agcccggttt ttttactgat tacaacggaa aaccatcaat
     7921 cccggttgct acaggcggag aagtttatct cattgacgat gttaatgcag gaacgctcca
     7981 catctacgaa acagtcaatg ctttcatcga aaacaccatg tgcaaaacct atgatgagaa
     8041 gtagagttaa agctctaaag taaataaata tcaagtaaat tgaaaaagaa cgacacatac
     8101 atataatacc aagtgatttg tttgtttaaa ggagatttcg atgaagggaa aagctgagat
     8161 cattgaatat tggaaggaac ttgctgaata tgatctcgag acagcaaaag caatgttaaa
     8221 gacaaaaagg tatctttatg tcggatttat gtgtcatcag gtcattgaaa aaatatttaa
     8281 gggttatttt gtttcaagaa aaaatgaaac accacaatac acacataatc ttttgaaact
     8341 ggcaaaagcg ggagattttt atggtgaatt ttctgatcgg cagaaagatt tgatcgatta
     8401 tcttgagcca ttaaatattg aagcgagata tccgagttat aaagaagcct tgctgaaaga
     8461 attgaccaaa gacagaatcg aaaaaatact taatgagaca gaggagttgt acgaatggat
     8521 aaaagagaag ttttagaaaa attgagaaga ttttcagaga ttgttgtaga aaaatacaac
     8581 cctttgaaaa ttgttctttt cggctcttat gcaaaaggaa caaataaaga ggaaagcgac
     8641 atagatgttg ctgtaattgt tgagaagatt gacggcagtt ttttagagaa agaagcagga
     8701 ctttacaaaa tcagaagaaa cattgatgaa aacattgagc cggtcctttt cgaagccggt
     8761 tcagataaaa gcggattcct tgaaagcatt ctcagttacg gtgaagtgat ctatcaaaga
     8821 taatatgtgc catataatca gtaaatcaca aaattttctt aatttcaaat cctttgctga
     8881 gtgctccgaa ttttgagggg caggagggga cgcattcgcc gcatttgatg cagtctattg
     8941 aattgatctc ttcggggagt ttgagtccca tggggcatga tttctggcag gcgcggcagg
     9001 tgacgcactt atttttatca agtttgatct gatatagtga gattttgttg aagaatccga
     9061 gtatgagtcc aagcgggcag atgtatcggc agaagaaccg tttttctctc attatgaaga
     9121 tgatgaacac tgtggtgaga atcagtttcc agtaaaatgt aattccgatc gatgccctga
     9181 tcccttcgtt tgccgcgatt attggaagcc ctgccataat gagtcctgac gggcatatcc
     9241 atgcacaaaa cccctgagca agtccgaatg cgggaacgat gatgaccagc agaatgagat
     9301 ttccccattt gatgtatctg aacggttgcg gaagtttcgc agtgtcactt ttccatggtg
     9361 aaattgagtg aagaagttcc tgaaaaagtc cgaaagggca gacatatccg caacttgcag
     9421 aaccgaaaac agctccgatt gctgaaaggg ttccgagagt atagtaggga atcattgcgg
     9481 catattttat tacatactga attgtgccga gtggacatcc gaaagccatc gaaggacaac
     9541 tgtagcagtg aagtccggga gcgcagaacg attttgcagg cccgttgtaa ggtcttccgg
     9601 tcataaaatt ggaaatgaac gggttctgga tgatgaagaa aatgagctga tatatccttc
     9661 tactcattgt cttttttctc acttgttccg aaaagctttt caaaaatgtt ctcatcggca
     9721 agtccgatac agctcatgca cacattttta cctgccgcat tcacaaggtc gatctctttg
     9781 gtcccgtctt catcaatttc atgaacccac cctttgatga gaaaaatgag tgaaattatc
     9841 aatattattg agacaatgac gatctttttc attttttatc tgtcttattc attgcaaaaa
     9901 ttgccgctcc aagtgccccc attatctgcg aaaaaccgct tgtaagtata tccgatttga
     9961 aaagtttctg aagatgaaat ttcaccggtt ttatctttga aactccgcct gacatgaaaa
    10021 aaggatcaga tggatgggtt cttcgtgcca tattataagt gttccttgcc accatttcag
    10081 caagagtgga tgcgatcacg atgtcaggaa catctcttga tatcattgaa atgatttcac
    10141 tttctgcaaa aactgtgcag gttgagttta ttttctgcga ctcaattcct tcaatgtcaa
    10201 gcaaagcaaa cttttcaagg tcaaaaccga tccgtccgca catcacctca agaaatcttc
    10261 cagtgccggc ggcacatctg tcattcatta caaagtcttt aattcttgaa tcattatcaa
    10321 ttgttataac cttgctgtcc tgcccgccga tatctataac ggttcttgtt tcagggtgta
    10381 gtgagataac tcccagatag tgtgccgtga tctcagtaag tgtgaatgtt gaagggtcaa
    10441 aaacagactc tcttccgtat cccgtaacag cttttgaaac gatatttttt ttaatttcat
    10501 ctggaatgga gttagaaagt ttgtttatcg tatttgtcgc gccggctatt gtctcgattg
    10561 ttgatgaaaa aactatctct ttaatatcat tgatgataac gatcttgcca gttcttgaac
    10621 cgatgtcgat tccgcaaaag agtttcaatc ttttttaagc tcctttttag ctttctgcca
    10681 gagttcttca agttctttaa ccggtcttcc accttcgatg aaagaagggt ccagatcttc
    10741 cattttatca aaccgcgcct taaacctttc tgcacacatt tttaaagctt tctcattgtc
    10801 aacaccgagc tttcttccaa gattgataac agcaaaaaga aggtctccga gttcatgctc
    10861 tatatgtgcg gcatcatttt ctttaactgc ttcaaggagt tctgcctgct cctcttctat
    10921 cttatcagtc acatcttccc atttttccca gtcaaaaccg acacttgccg ctcttgaagc
    10981 atatctttgc gcaagcagta cagcaggcat tgatcttgga atgccgtcaa gaagatgttt
    11041 cctctcttcc ttttttcttt ttatcagatt ccagtttgtc agcacttcag cactgtttct
    11101 gcaatgatct tcaccgaaaa catggggatg gcggcttata agtttatgtg tcagagtttc
    11161 agcaatgtca ttgaaatcaa aaatgttctc ttcacctgcc atctgagcaa tgaaaacaac
    11221 ctgaagaaga agatctccga gctctttttt aagctcaatg accgattcag gaatagatct
    11281 gtcaaattcg tcaagggctt caaccgtttc aaatgcctct tcgattatat atggtctaag
    11341 tgactgtatt gactgttctt tatcccacgg acatccgttt tcaccacgga gcacagccat
    11401 tacttcaagg agttttgtga tgcctttcat ttcagacatt tcaacacctt ttgtcgggcg
    11461 aattcacttt ctggtgaaaa tcttcaggtt tgtattcgta tttgagattg aaacaggcaa
    11521 aacagaaatt gtcagaattt ttaaggatct tttcaaagtc ggtcacttca atatatctga
    11581 gcgtttccgc ttcaaggaat tggcagatct catcattgtt cttgatggcc gcaatgagct
    11641 cttttcttgt cggagtgtca attccgtagt aacagcttcc gatcactctg ggggaaccga
    11701 ttctgacatg gacctctttt gcgccggcgc ttcttatcat tcttacgatc tttcttaaag
    11761 ttgttccgcg aacaatagaa tcatcaacaa gcacgacctt ttttcctttg aaaaattcag
    11821 gaagcggatt gaacttatgt cttacccctt catcccgctg tttctggtcg ggttttgtga
    11881 aagttcttcc tacataatgg tttctcaaaa gtcccatatc aaactgaaga ccgcttccgt
    11941 gggcataacc taatgccaca aagtttgatg aatcgggaac tgccattacg acggtgtctt
    12001 caccaaaccc gagctctttg tcatattcag caagtcgtgc accgatcttt tttctgacgg
    12061 catatacttt ttcttcatac actatcgtat cgggacggga aaaatatatc agctcaaaaa
    12121 cgcagtgctg tttatttttg ctgttgacag ctatcgtttt catcggcttg tctttttcga
    12181 gcttgatgat ctcacccggt tcgagctcgc ggatcatttt tgctcccact atgccgaaag
    12241 cacagctttc tgaagcaaga acaacaccgt ttgaggaata ttcacctgtt tcgcttacac
    12301 cctgaagatg tccgagaatg taaggcctgt aggcatgagg atctctgaat gcgaacatct
    12361 ggtcgcggta gatcacaata gatgaaaaag cccctttcaa ctggctctga gtttcgacga
    12421 tggcgttttc gatggtcatt tttctgtgta tgagattgaa agcgatcagc cggccgatga
    12481 gctctccgtc gttatcgctc ttgaaagtga actcgtactc tttttcaagc catgttctca
    12541 gttcgtagta atttatgatg tcgccgttac tgcaaatggc aagatgcgga ccttcaggaa
    12601 gttctataac atggggctga ctgttcgttt cgtcacttct tcccgctgtc ggatagcgga
    12661 catgaccgat tgcgatcttt ccctgatatt ttgcaaggat gctttcggta aggacattct
    12721 tgaccagtcc cattcctctg taacctgaaa tgttcccgaa ttcatttttt gaagcaatgc
    12781 cgcagctttc ctgaccgcgg tgctgaattg caaaaagtat ttcagccaca agaatttcac
    12841 cattttcaac attaaatacg ccggcaattc cacacacagt aacctcctga ataagtacca
    12901 aaaatctggc taactctact cataataaaa tctaaaatca aggtgaatga tctaaaaaaa
    12961 aataaatata tcaatcttgc tcaatttaaa caattatggt atacagtaag ccgttttttt
    13021 tggagtgagc gatgatcacc gaatgtccaa gttgtaaaaa agtaatttct gtaaatgttc
    13081 cgggaactgt tatgtgtccg aaatgtaatt ctcttgtttt cattggcgat cctttgaagg
    13141 atgaagagaa tagagttata aagaccgccc gtgaacttaa actcgaacgc gatgtagaat
    13201 catcaaagaa aaagatcgag gacgagaacg gtttcttcaa tcctttcggg gagatgaaga
    13261 aggttaaaaa agcaggtttc ttcaaaggga ctccgtggga cagatggcac gagctcggtt
    13321 ttgcggaagc attcataaaa accacaaaag agatagccac aagccctgaa aaatttttca
    13381 aggacatgaa acatgcggcc aatccgggat tgatgccgat atacgggata atccttgctt
    13441 ttctgacagt gcttttccag acattctggg tacttaaact gttccagtcc tatttccccg
    13501 attttgcgac attccgggac acattcctta aaatgggaga tctgggggca tcaatatttg
    13561 cagacgagaa caagctcaga atgatatatg accagatgta tcccgacggt ggctcgcttt
    13621 tttcccacct tgttcttaca cccttcatgt cgatagtcgt tacagctttc atcctgcatc
    13681 ttggcagcgt aatgctcggt tcaaagaccc ggctgaccca tttctacaga atgagcagtt
    13741 tcataatggt cacaggtctt ttcagcattt ttccggtcat aggtaacatc gccggctttt
    13801 tctggagagt tttccttgtc cataaagggg ggatggcact taataatttc aacggaagaa
    13861 aggcatatat tttcacaggt ttttatgtag ccatgcagct tttcttttcg acagtgggga
    13921 taatatgatc tttagagtcg gacagggttt tgatgtgcac aggtttgaag agggccgcga
    13981 actttatctt ggcggagtga agatcgacca caccggtctt gcaggtcaca gcgatgcgga
    14041 tgtgcttatc catgcggtga tagatgcact tctgggtgca agtgcgctcg gagatatcgg
    14101 cgaactcttt cctgacaaag atccggaatt taagaatatt cgaagtactc tgctcttaga
    14161 gaatgttgtt aggaaactta atgataataa ctggttgata gaaaatattg atgtgactgt
    14221 aatatgtgag acaccgaaaa tatcgcttta ccgcgaactt atcagagagc agatttcaaa
    14281 gataacaggt atcgggactg acagaataat ggtcaaggga aaaacgactg aaaagctcgg
    14341 tttcacagga agaggcgaag gaatagtcgc aatggcagtg acacttttaa aaaaggagga
    14401 gtgattgaaa aagatatcgt tctacatcgg caggaagaac accgtcacat tttttctggc
    14461 ttctgcggtg cttcttttcg gaatatattc tttctttggc gaagttgtct atttcttttc
    14521 aggttcggaa gcgatagacc ttaaagatgc catggatata gacaaggcga attttgaaaa
    14581 ggtcgatgac ggtgactttg ttcaggtcaa agggataact tcggtacagg gcggtgctat
    14641 ccagagagga atcaccggaa cagaatacat tctctactat cttaccggtt cgtcaaagtt
    14701 cattataaac gaaaagaatg acggtgaatc taaaggcccg cagtataaaa ctgtaagagg
    14761 gcgtgcatac aagttcaaga caaattcaaa cgccgcaagg atgaggaatt tctttgcaag
    14821 gtcgttcatg atagaaatgg atgaagacgg gatcctgatc gaagccgggc ttgagccggg
    14881 gagtgattat gccgctctga tcttttttat aatgctgatc gcggccatgg ggctgaacat
    14941 ataccttttt ataagacctc ttaaacagaa agaagacccg atggagaatg acattgatga
    15001 aatttgagaa agtaagaaca agatttgccc caagtcccac cggatacatg catgtcggca
    15061 acttgaggac cgctctttat gcatggctga tcgcaaggaa gaataacggc acatttattc
    15121 tgagaattga agacaccgat caggaaagaa aaatagaggg tgctgtcgat ataatttaca
    15181 gaactcttgc cgaaacaggg ctcaaccatg atgaaggccc cgatgtaggc ggagattgcg
    15241 gtccttatgt tcagagcgaa agaatgtctt caggaatata tgaaaaatgt gctctggagt
    15301 taattgaaaa aggtcatgca taccgctgtt tctgcacaaa agaacgactc gatgcattaa
    15361 gggctgaatg cgaagccaaa gggacacaga caagatatga cagacattgt cttgatctgg
    15421 gtgaagaaga aataaaaaag aatcttgaaa acaaagtccc gttcgttgta aggcagaaaa
    15481 tgccgttgac cggaacaact tcatttcacg atgaagtata tggcgacatc acaatagaaa
    15541 attccaccat ggatgatctt attttaatga aatcagatgg ttatccgaca tacaactttg
    15601 caaatgtcgt tgatgatcac cttatgggga taacccatgt tgtaaggggg agcgaatatc
    15661 ttatctcaac accgaaatac aatcttcttt acgaagcttt cggctgggag aagccgtttt
    15721 atgtccatgt ttctccagta atgagagatg cgcagagaaa gctttcaaaa agggacggcg
    15781 atgcaagtta tgccgatttc attgaaaaag ggtgtcttaa agaggcactt ttgaactatg
    15841 tcgcacttct tggctggaat ccggggacag atcaggaaaa gttcactatt cctgaattga
    15901 taaatgcttt tgacattagc ggaataagca aatctcctgc aatatttgat gaagcaaagc
    15961 taaggtggct caacagtgaa tatataaggg cgatgtcgca cgaagagttt cttcagcacg
    16021 caatgccgtt cattaaaaaa actgttaaaa gggatgatgt caattttgat gtcattgcgg
    16081 aaggacttca caaaaggaca gagtattttt cagacatacc gcctcagctt gatttcattg
    16141 atgaacttcc tgaatatatt gcagaactgt acgaacataa aaagatgaag acagataagg
    16201 tgaattcgtt gcaggcactt gaaataatgc tgccggcact tgaatcgttt tctgactggg
    16261 accacgaaaa gctttatctg atgctcaatg accttgctga aaagagcgga attaataaaa
    16321 agatcatcct ctggtcgctc agagtcgctc tcagcggcaa agagcttact ccgggtggag
    16381 ggactgatat tgctgcgata atcggaaaag aggagtcaat aaaaagattg aaaaaaggga
    16441 tagaactgct gaaagtttaa taaccatatc gggagaagaa aaatgacaga catcaaaaca
    16501 aattatctcg gtcttgagct tacaagcccg attgttgcgg gctcatgcag catcacaagt
    16561 tcagttgaga atttaaagaa actggaagtg gcaggtgcag gggcggtagt gctcaaatca
    16621 ctttttgaag aacagatagt cgaagaaagg atcaaaagtg caacgcaggg aatttattcg
    16681 ttttttcatc ctgaatcaat ggaatacatt tctcagatga gtatgaattt cggtccggaa
    16741 tactatcttg accttattaa aaatgccaaa aaggagatat ccatacctgt cattgcaagc
    16801 atcaactgtg tttcaaagga atactgggtg gagttcgcaa aaaaaattga aagcgccgga
    16861 gccgatgcta ttgaacttaa catcagccat gtaacccatg atattgaaaa aagtgccgac
    16921 tcggtgacgg acgattatct tgcaacattc aaagcggtaa aggaaggggt gaggatcccc
    16981 gtttctgtca aaatggggca gaatttcaca aatatagcaa gaatggtcaa acttcttgaa
    17041 acccacggag cttcaggagt tgtccttttc aacagattct atcaggttga tattgatatt
    17101 gacaaacttg agcttgttcc ggggtatgtc cgttcatcaa gaaaggatat cactgaaacc
    17161 atcaggtgga tcagcattat ttcaggaaag accaagcttt cactttcggg ctcaagagga
    17221 gtatcggacg gtcgtgatgt aataaaaatg atacttgcag gtgctgacac cgttcagaca
    17281 gtaagtgctc tttatcagaa cggcattgac tccattgcaa aaatgaatct tgagatcctt
    17341 gactggatgg aaaggaaaaa tttcaagtca ttaagtgaat ttaaaggtct cttaagccag
    17401 aaaaagatcg ataatcccga tgcgtgggaa agacagcagt atatcaaagc tctcgtcgga
    17461 atagaataaa ggatctatat aaaggacaaa tggtctgcgt ttattccgat ttatttgaca
    17521 acgattaaaa tcagagcata tatttcaact gtgttgttca acttttcatt ctggagattg
    17581 agtcatggat tttgtaaaaa tggtgcatga aaaagctgtg aaagccggaa aaactattgt
    17641 gcttccggag ggttgtgagc caagaactgt aaaagccgct actatcatta caaaagataa
    17701 acttgcaaaa gtaattcttg taggaaatga ggcggatata ctgaaaacgg cacagaaaga
    17761 gaatgtttct cttgacggga tcaaaattgt cgagccattg aaaagtgagt ggtatcagga
    17821 ttttgtttca accttttatg agatgaggaa aaacaaaggt gttgatatgg gcagagccgc
    17881 tgaaataatg aaagatgaac caaaatcgtt cgattctgac ttaaactccc gaaaaatggt
    17941 ctgagctcag ca
//
LOCUS       DAOOYB010000025        17934 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_3030_length_17934_cov_11.900833, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000025 DAOOYB010000000
VERSION     DAOOYB010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 17934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17934
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3030_length_17934_cov_11.900833"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(352..531)
                     /locus_tag="PL056_02610"
     CDS             complement(352..531)
                     /locus_tag="PL056_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45940.1"
                     /translation="MQKPQDKETRYFIDLDLKTMKIIGWNYDQREELVKQKLPPSKVR
                     IYITKGQYNKLVKEG"
     gene            683..2008
                     /locus_tag="PL056_02615"
     CDS             683..2008
                     /locus_tag="PL056_02615"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM45941.1"
                     /translation="MRDLLLKHNKHWSGTGIETGIKREITDLLLAAIDVNHIIAISGA
                     RRSGKSYLFRQLLSHLLKNSIPAANILFLNFEDPFFATQANNAEILDKIFTEYKVLKN
                     PEGRTYLFFDEIQNIQNWQLWVRELYDRDDTVKIFITGSNSEMLSVDLATHLTGRVLA
                     FENFPFSFREFISGMESFLLPDNIDYESLFPVRETIMHLIETRFEKGFFPETAYLESK
                     ELTYDVLSQYFQNVIFKDIIPRFVIRNSKTIEQLAYYCSNTFAAKFSYRRLAEAVSSN
                     ENTIKEYLSYFEKAYLFFVVEHFEYSMRKQLKYAKKLYISDSGLRNATTSSFSPDQGK
                     HAENVVFTHLRKMSKNINFWADEKSGKEIDFIVKKKDKYLLCNVSYTDELPEREFQAF
                     PLFLKLSKMKNYECLVVSKGKFSTQIYDNITINTIPLWLFLLTLNKEDF"
     gene            2096..3538
                     /locus_tag="PL056_02620"
     CDS             2096..3538
                     /locus_tag="PL056_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45942.1"
                     /translation="MTNPYKVRTETESVAPKHVPKEQWEGYPNPVWHKKDKKKVTILY
                     SMIERRKSFFIRGYLIRHGYKFIDMGDHIKEDVRWGKEYGNRMECNPMYFTSGSFIRH
                     LFKLHNELGLSKEEVVDKYVFMSGGGQCGPCRYGMYPQEYLKVANDAGFKGFRLMIFS
                     SELQDSLLPKECAFQFNLNFRINLLIAFVLADLMHIAECALRPYAVDREHALKVIEDC
                     ENILLEAFTSRLYLLKLPEALRKVGKMLADVPKKAVKLPLIYMTGEVFANLAHNEGNY
                     NMRRFIMDEGCEVIPGSFTSRAMYDNWRRSREAERGMKYAATKKEYKYWKKSLKRQKV
                     SDKAILLIYNWFKKNLNPEQFGGRAKLPDLDELARLGYEFYHPEIFGGEGNLEIGEAV
                     HYAGIVDGFLSSKPFGCMPSSGVSDGVQAKIMSMYPDLNFLSIETSGDNEVSILSRVS
                     MMLFKAKEKAGKRNSQQDIADIEALNNVKD"
     gene            3525..3692
                     /locus_tag="PL056_02625"
     CDS             3525..3692
                     /locus_tag="PL056_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45943.1"
                     /translation="MSRIEFVLREIQQLIEFYDKTKELREKIKEKSKEKNRYVVQDKL
                     YGVVSEKSEKD"
     gene            3804..3992
                     /locus_tag="PL056_02630"
     CDS             3804..3992
                     /locus_tag="PL056_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007815142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HPM45944.1"
                     /translation="MFKKNVGSVDKVIRIVLGIAIIGAGIYYGSWLGAIGVVPLLTGI
                     FGTCGLYSLFGINTCKIK"
     gene            4011..4382
                     /locus_tag="PL056_02635"
     CDS             4011..4382
                     /locus_tag="PL056_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015589919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HPM45945.1"
                     /translation="MILVSACLAGIPCRYNGTAKPDSRIIEMVRAGLAIPVCPEQLGG
                     LTTPRIPADIRGEKVVSKNGVDVTAQFNKGAQITLEIAKLYDCREAILKSRSPSCGKI
                     DGITAKLLKKNGIIVKTEEEL"
     gene            4383..>4747
                     /locus_tag="PL056_02640"
     CDS             4383..>4747
                     /locus_tag="PL056_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45946.1"
                     /translation="MNIHPFLDNLIVKMMKAVAGKRRIFSKSIESPSFAELMPLISLK
                     VPKMDGVGIYQVEAETPAGLMNPAFKVQQWLGNDFPTIIYHHGNSERPFKIRRFARNT
                     FFKIFINTGNPVEANLIAL"
     assembly_gap    4748..4847
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5029..6546
                     /locus_tag="PL056_02645"
     CDS             5029..6546
                     /locus_tag="PL056_02645"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HPM45947.1"
                     /translation="MFPAGKAVDAREFDKRAVERGIPSLILMENAAFSLFLEVSRVID
                     EFKPEKIVVFAGKGGNGGDGFALLRILKDRGCNIPMYIVPFFTVTPLHNSTGAINRAP
                     ALTTHDSPFTVHDSLTNWKMLPKSVEITGMENISGKVLFIDAMVGTGLKDDLTGRMRE
                     GVEFINKYNDKFVVAVDIPTGLNSDTGIIMGVAVVADLTVTMGIYKTGLFASKGPSVS
                     GRIVLGKISAIDDEKDQYTYFVEENVVPELVDIPIDSFKNKNGHLLIIGGDLDKLGAT
                     IISARSFMVSGGGLVTAAFSKDLHEKIAGVMPGMMLADIDEVPKKLHQFSSIIIGPGL
                     SKWPFEKHDIFKNYKGILIIDAGMFDIMNEFNDILESLKDLKVVFTPHPGELKRFLKA
                     HTDEPWINSVERFQLKKDHILVAKSHSTFIRSVEKTVIIPHGAKALSFGGSGDALTGL
                     IAYETNFSGFESGVKRAVLRHRMAGIELEKRYSATFHDIGKLIELIGITGKKDVC"
     gene            6536..6976
                     /gene="tsaE"
                     /locus_tag="PL056_02650"
     CDS             6536..6976
                     /gene="tsaE"
                     /locus_tag="PL056_02650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HPM45948.1"
                     /translation="MSVEVHSVKNESETALIAKNIASEIKGGGLITLDGDLGAGKTFF
                     TGALCEALGLERKSVSSPTFVIMKKYSGIVDVYHWDFYRICSVDELYVADFFEYLSET
                     GSITIVEWASMFKECWENHLKRIEIKIEFGEKEDERRIEIKRNF"
     gene            6942..7946
                     /locus_tag="PL056_02655"
     CDS             6942..7946
                     /locus_tag="PL056_02655"
                     /inference="COORDINATES: protein motif:HMM:NF018917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HPM45949.1"
                     /translation="MNDESKSKEISENSDETQEESIFNRPRQKLSIAKERNQKPETPP
                     PPKHEMAIKEEEKKPDNVEIVKEKVSKAVEVTKKKIDETLKGGTSLKTAAKAIFSNGK
                     LTVASLTVVLAMVLITIFSYHQISSWVDSLTAPFFDKEPLTEGFFDYIYYFGWLITRM
                     VFKAVAAIVVFYISFIFSYTVTSPLYSFISVLAEDIYFGKPEDATDFSLDIMIEDVLQ
                     ALKIAGMALVCTVFAFFVNFVPVIGQITAIAFYIVINSLMLIDFPASRRSWDLKTKTL
                     WIRENLMTTLRIGSLPTLISMIPLANNIILAFCFPLFVVHATMNFAEVEKNRTDKSIS
                     "
     gene            complement(7939..11145)
                     /locus_tag="PL056_02660"
     CDS             complement(7939..11145)
                     /locus_tag="PL056_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45950.1"
                     /translation="MKRALILFQFIFAVSIVHAADSVAVFPVGAQNRGEWTVAKDVDR
                     FLIENFGKISGAEPVSFKELLSTNDRKALNKCREDLKCQQNISRKISKKVDFFLFTRL
                     KIGSEITVNTYLFDRKYNRLESAKIDLDEFAEADTIADEIITYWNRFLKKHTSSSGIS
                     DSDFSTERKSKKAAKKQKYENEDSDYEEEEEDASSRSSKSRVVSVENLIMSGFTSYAE
                     GNLKEAESYFEKAASRDVVAKKLLNNVQDAIKFTARGNAAIKAKNHEEAVLMISKAEK
                     ANEEITGLGVKYRAYSKDSSVERVTYLEPTSKDIQIVDSIHRKYSKLTEKSRKDKAAK
                     LAEIDKWLNGRILERESKLKQFETDSKNAVELEKKEYADLAKKIKDLKYQWEKDDSEI
                     EQKIVALENKLTLFEQREKGVVKVSTEGDEKARKDEMDAVDKKYTELLKKMRTEKEQF
                     ENKQKEEIEKGSKANEAAVVKLEEKKKANDAKIVEIDKKIAAEMEKFDAEEKKFSGGN
                     EEVKMKNEDEDRKFKVQVEKEYQVKFDELNKKLQDFDQQESEKRKDLEKYDKAIEEYM
                     TKNVELMSKFQEELEKERATVDAEYKTKKESATAEAEKKYTEDLAKLTTEKTDLEAKI
                     AEKETPQLKKQLAAVEKKISAHESGKETFIADQVMKVDMEYEAKSMEIDTKLAKKNDE
                     LQKDNNKFRTAKLAEKKKAEGDFGGFQKRKDMFKKSIDDQIALAQRERDRKIEDRQKD
                     RTKLSGSWDSDRQKRQANLDRKLKVDRDAKDRLIKDNEKIAADITAVNTKWENTSQDL
                     RASQQTAFEKFDTQWKEKYEKTEAEYKEKKEAVDVKYAQKQVSEKEAQKQQKIKWEEE
                     IQSLNEEKKRRKDERAKILENEKTNWDTKKKQWVEEEKQRKIDKDQFVKDMKKMNEDD
                     RKEASQKRKDADAKYEESTKDIYQKEIDEISNKFREEYKITRTREVTGVKAQADIASL
                     KAEALAKNGLVKLDEKDLLGARRAFAEALFLDKNNQIALDGMKSINISAKSMYWEAYG
                     MRETNKNKAREIFTLLTKTLMPANEYFVKSRIALEDLN"
     gene            11351..13027
                     /locus_tag="PL056_02665"
     CDS             11351..13027
                     /locus_tag="PL056_02665"
                     /inference="COORDINATES: protein motif:HMM:NF004952.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HPM45951.1"
                     /translation="MSELTKKGFGELLEEFLPSEKEVSSEEICKGVVRAVRKDFILVD
                     VGLKSDGIIPSSEFTAEEISKLKVSDEIEVVVDRWEDEKGNICLSKEKAGKLKVWEVV
                     EKAFDDGSLVNGTIVARVNGGLAVDIGVKAFLPNSQVALRPIKNVDDLIGKNFDFKVI
                     KFDRKKGNIVISRRDLLEKEREVLKNTTLEKLQEGAVVKGLVKNITYYGVFVDLGGID
                     GLLHITDMSWGRVNHPSEMFKIGDEVDVKVLKYDRDSERVSLGVKQISEDPWIHASDK
                     YASGKTVKGVIVSLEDYGAFVELERGVEGLIHISEMSWTRRVKHPGKMVAIGDEVEVK
                     ILEIDVENRRISLGMKQIEPNPWELLREKYPINTVVRGTIRNITDFGLFVGVEEGIDG
                     LVRTTDITWDRKLKHPSEYYKKGDEVEAVVLNIDAEKQRFSLGVKQLSEDPWKMFKDQ
                     HNIGEKIEVEVVKIADFGVFVKVFDTIEGLVHKTEFEDENKEYQPGDKVEVIIKSISD
                     TDRKVSLSVKAVSDEDAQKEIRDAAANEKVPEKLGSFADAFKNAEDAEKK"
     gene            complement(13077..14348)
                     /locus_tag="PL056_02670"
     CDS             complement(13077..14348)
                     /locus_tag="PL056_02670"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HPM45952.1"
                     /translation="MTTTHPQSRLTHLPMQDVAFPATFAADFISELAKAVEKDKEGHD
                     SYHSWKVCIIACKIGEALKLEKSRMKELFFASLLHDIGGVMISGHIIESLVQVPDIFG
                     QKNDFFVFAHSHRGETILRSFPTFRKISEIVGAHHEFFDGSGFPNGLKGEKIPLLSRI
                     IRVADSIDIFSRLHKIDDTGELILFLNMVSGEEFDPVIYNTFVKLAENGLLNLINTED
                     DIKNEMRSLKKQMDDRYYFSSTDTVNRFFKMVAMLTDNLTSPQDNHSMRVAELSVQIA
                     YLMGLDEEEILAIRWAAFLHDIGKISGDRSIYLKKEKLSEEEWLSIRMHPQRSYDIIN
                     SISGMDKIAYYILHHHENFDGSGYPESLSTKMIPAGSRIMRVADAFDAMTTNRIYHRK
                     KDWQRALSELKKFSGTQFDPDIVDVFINDIT"
     gene            complement(14349..14765)
                     /locus_tag="PL056_02675"
     CDS             complement(14349..14765)
                     /locus_tag="PL056_02675"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPM45953.1"
                     /translation="MDRKNITILILDDEENILSSLKRILEGTGYTVVTKNKPEDAVDF
                     VRNNVVHIALVDVLMPNMDGITVLNIVKEISGLTQVIMMSAYSTIDRIVSALENGAND
                     FLLKPFDNIELFISIVEESEKKLLRWQDILSQTGAI"
     gene            complement(14783..15367)
                     /locus_tag="PL056_02680"
     CDS             complement(14783..15367)
                     /locus_tag="PL056_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPM45954.1"
                     /translation="MTYLPLSYLLGSRAKVDILRILVNKQNYTGRKIATLSSTNPNSN
                     KKALDFLIETGIVDREHIGNAFHYRLNENHLLYEPMVNLFKEEEKTVNRIMKTGASFI
                     EEYAPKTLAGFVSYLRNDVVLTVLAHSFPRDEMKDYIFEKTGFRVLIKMIDTLNLNEN
                     DLGNFVDRVYFWNNYKSIMKMINSRDVVKNFFNF"
     gene            15570..16985
                     /locus_tag="PL056_02685"
     CDS             15570..16985
                     /locus_tag="PL056_02685"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HPM45955.1"
                     /translation="MSAKKRSEKSFDPKSLLYTKSSGWDGISKNEMQKVSDFSDGYKK
                     FLDNAKTEREAVRYIQKIALSKGYKEITKAKPSDRKLFINFDNVSCVLAEINDTTFNN
                     GFLMVGAHIDTPRLDLKGNPLYEAEDMAYLKTHYYGGIKKYQWVTRPLALHGVVVTEE
                     GELKDITIGEDDSDPVFTINDLLPHLAQDQMQKNARTVIEGENLNILVGSVPYKFKHE
                     KDSIKTAIMSILYGKYGIKEEDFVSAELQLVPAGKARDVGLDRSFVGAHGQDDRVCAY
                     AGMEAIFDSGKSSKNKIAMFFDKEEIGSKGNAGANSNILERAVNKIINLYGKGNYNTL
                     INSLADSKFLSADVNAGIDPEWAGTMEKMNAAKIGFGVCLTKFTGSGGKSNSNEAHAE
                     FVAECRKAFNKDKVKWQTAELGKIDLGGGGTIAMFIAEYGMDVVDCGTALLSMHSPFE
                     IASKADIYHTYAAYKAFYKYL"
     gene            17075..>17934
                     /locus_tag="PL056_02690"
     CDS             17075..>17934
                     /locus_tag="PL056_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013407033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HPM45956.1"
                     /translation="MTGNELIQIDEIKRRIFTIRGMQVMLDSDLAELYGVETKHLNRA
                     VKRNIERFPESFMFQLAETENLRFQNGTSRQHGGRRYLPNAFTESGIAMLSAVLKSDV
                     AIKVSVQIMEAFVAMRKFINQNAEVFFQINSVEKKLVDYKTETDKKLSFIFKTIEANE
                     LPAKQGIFFEGQIFEAHKFVSDIVRSAEKSIILIDNYVDDTVLTLFSKRKRGVALKIL
                     TKNLSKQLQLDTQKFNEQFPPAEIKEFSYSHDRFMIIDDCDLYHFGASLKDLGRKWFG
                     FSKMDVEVAK"
BASE COUNT         5260 a         3345 c         3667 g         5562 t
ORIGIN      
        1 aaattataag tccgatcttc caatacaaat ccaattgaat tgaattagcc gctttaacag
       61 ctttgatcct tgaaagttct atcgcactct taatcgaaag aaacgcttcc ttatagtttt
      121 tcggcatcaa atcgttcaca tcaaacctcc tcaacctgaa aatttgcact gatcaaccgc
      181 tcttaaatat atatcaacca atagataatg caacaattag attaatatta caagagaagt
      241 gataagatta tcttactcgc cggaaaactg gggaaagtgg agtttattta gggagttggg
      301 atggatttaa gacagaaaaa tatgtgtaga aacatgggtc ctgtaaacta cctaaccttc
      361 cttcacaagt ttattgtact gtccttttgt aatgtagatt ctgacttttg agggcggcag
      421 cttctgtttt acgagttctt cccgttgatc gtaattccag ccgatgatct tcatggtttt
      481 gagatccaga tcaatgaaat atcttgtctc tttgtcctgc ggtttctgca tgttttcctc
      541 cattttttta aaaagtcgtt gaattttaca tccgcacttt gattttacaa ggaaatgttt
      601 cttgcaaaaa tcggcttgac atcgtaaatt tgcgaattat aattagcaaa tagaccgtta
      661 tttgcgaatt atggagagct caatgcgcga tttactgcta aaacacaata agcactggtc
      721 aggtaccgga attgaaacag gcataaaaag ggaaattaca gatctgcttt tagctgctat
      781 tgatgttaat cacattattg ccatcagtgg tgcaagaaga tcggggaaat cgtatctttt
      841 tagacagtta ttgtcacatc tccttaaaaa ttcaattccc gcagcaaaca ttctcttcct
      901 caactttgag gatcctttct ttgctacaca ggcaaataat gccgaaattc ttgataagat
      961 atttactgaa tataaagttc tgaaaaaccc ggaaggaaga acatatttgt tttttgatga
     1021 aattcagaat attcagaact ggcagttgtg ggtcagagag ctttacgaca gagacgatac
     1081 ggtaaaaatt tttataactg gttcaaactc agaaatgctt tctgttgatc tggcaaccca
     1141 tcttaccgga agagtgcttg cctttgaaaa ctttcctttt tcttttcgtg aatttatttc
     1201 cggaatggag tcatttttac ttcctgataa tattgactac gaatcccttt ttcctgtacg
     1261 ggaaacaatc atgcatctta ttgaaacgcg atttgaaaaa ggctttttcc ctgaaacggc
     1321 atatcttgag agcaaagagc tgacttatga tgttttaagc cagtattttc agaatgttat
     1381 atttaaagac atcatcccca gatttgtgat acgaaacagc aaaactattg aacagcttgc
     1441 atattactgc tcaaacactt ttgcagcaaa attttcatat agaaggcttg cagaggcggt
     1501 ttcaagcaat gaaaacacta ttaaagagta tctttcatat tttgagaaag cttatctttt
     1561 cttcgtagtg gagcattttg aatattcaat gagaaaacag ctgaaatatg caaaaaagct
     1621 atacatatct gacagtggtt taagaaatgc cacaacaagt tcattttcac cggatcaagg
     1681 aaaacacgct gaaaatgttg tttttacaca tcttcgcaag atgtctaaaa atataaattt
     1741 ctgggctgat gagaaatcag ggaaagaaat tgattttatt gtgaaaaaaa aagacaaata
     1801 tcttttgtgc aatgtttcat atactgacga gttgcctgaa agagaatttc aggcatttcc
     1861 attgtttctt aaattatcaa aaatgaaaaa ttatgaatgt cttgttgttt caaaaggaaa
     1921 gttttcaact caaatttatg acaacattac aataaatact attcccttat ggctttttct
     1981 cctgacatta aataaagaag atttttagaa gctgcttatt cttgcaaaaa tcggctgaat
     2041 ttatatgcta atccgttgta aataacgggt ctttttccaa ttttccgggg gtggaatgac
     2101 aaatccttat aaagtgagaa ctgaaacaga gagtgttgcg ccaaaacatg tgcctaaaga
     2161 gcagtgggaa ggatatccta accctgtttg gcataaaaaa gacaagaaaa aagtgacgat
     2221 cctttattcg atgatcgagc ggagaaagtc gtttttcatc aggggatatc ttatccgtca
     2281 cggttacaag ttcattgata tgggcgacca cataaaagag gatgtccgct ggggcaagga
     2341 atacggcaac aggatggaat gcaatccgat gtatttcact tcgggaagtt tcatcaggca
     2401 tcttttcaaa cttcacaatg aactggggct ttcaaaagaa gaagttgttg ataaatatgt
     2461 tttcatgtca ggcggcggtc agtgcggtcc gtgccgctac gggatgtatc ctcaggaata
     2521 tctgaaagtg gctaatgatg ctgggttcaa gggttttcga ctgatgatct tttcttctga
     2581 attgcaggat tcacttcttc cgaaagagtg tgcatttcag ttcaacctga atttcagaat
     2641 aaatctgctg atcgcttttg tccttgccga tcttatgcat atcgctgaat gtgctttgag
     2701 gccttatgca gttgacaggg aacatgctct gaaagtgatc gaagattgtg aaaatattct
     2761 ccttgaagcg tttacgagcc gtctatactt gttaaaactt cctgaagcat taagaaaagt
     2821 tggtaaaatg cttgcagatg tgccgaaaaa agcggtcaaa ctgccgctta tctacatgac
     2881 tggagaagtc tttgcaaacc ttgctcataa cgaaggcaac tacaatatga gaagattcat
     2941 aatggatgag ggttgcgaag taattcccgg atcgttcaca tcaagagcga tgtatgacaa
     3001 ctggagaaga agtcgtgaag cagaaagggg aatgaaatat gcggcaacta aaaaggaata
     3061 taaatactgg aaaaaatcac tcaaaagaca gaaagtgagc gataaagcga tccttctgat
     3121 ctacaactgg tttaagaaaa atctgaaccc cgaacaattc ggcggaaggg caaagttacc
     3181 tgatcttgat gaacttgcac ggcttggata tgaattctat caccccgaaa tatttggcgg
     3241 agaaggaaac cttgaaatcg gcgaagcagt ccattatgcc ggaattgttg acggattcct
     3301 ttcatccaaa ccatttggat gcatgccctc atcaggagtc agcgacggag tgcaggcaaa
     3361 gatcatgtca atgtaccccg acctcaactt tctatcaatc gaaacaagcg gcgacaacga
     3421 agtcagcatt ttaagccgag tctccatgat gctcttcaaa gccaaagaaa aggcggggaa
     3481 gagaaattct caacaggaca ttgcagacat tgaggcactc aataatgtca aggattgagt
     3541 tcgtactgag agaaattcag caacttattg aattttatga taaaaccaaa gagttgcgtg
     3601 agaagatcaa ggaaaaatca aaagaaaaga accgttatgt tgttcaggat aagctttatg
     3661 gcgtggtgag cgaaaagtct gaaaaggatt gaatctaagg taaaagaagt ggtttccccc
     3721 gcaattcaga cttccgggtc ttgtttttca ggataactgt ttattaaatg agtcagctta
     3781 tttaacaatt gatggagaga atcatgttta agaaaaatgt cggaagtgtt gacaaggtta
     3841 tcaggatcgt gcttggtatc gcaattatcg gtgcaggaat ttattacgga agctggcttg
     3901 gagctatcgg agttgttccg cttttaacag gaattttcgg cacttgcgga ctttattcgc
     3961 tttttgggat aaacacctgt aaaataaagt gattttttta aggtgtagaa atgattcttg
     4021 tcagtgcgtg ccttgctgga attccttgca gatataatgg aactgcaaaa cccgacagca
     4081 gaataatcga aatggtaagg gcaggtcttg cgattccagt gtgccctgaa cagctcggag
     4141 gtcttacaac tccgagaatt ccggcagata tacgtggtga aaaagtagta tcaaaaaacg
     4201 gtgtggatgt taccgctcaa tttaacaaag gggcacaaat tactcttgaa attgcaaaac
     4261 tttatgactg ccgtgaagcg atactcaaat caagatcgcc ttcatgcggc aaaattgacg
     4321 gaataactgc aaaactttta aagaaaaacg gcataatcgt caaaactgaa gaggaacttt
     4381 aaatgaacat tcacccgttt cttgacaatt tgatagtcaa aatgatgaaa gcggtcgcag
     4441 gaaaacggcg aatattcagc aaaagcatcg aaagtccgtc atttgcagag cttatgccat
     4501 tgatatcatt gaaagttcca aaaatggatg gagtcggaat atatcaagtt gaggcagaaa
     4561 cccctgcagg tttaatgaac ccggctttca aggtgcagca gtggctcggg aatgattttc
     4621 cgacaataat ctatcaccac ggaaacagcg aaagaccttt taagataagg agattcgcaa
     4681 ggaatacttt ttttaagata ttcatcaata ccggcaatcc ggttgaagca aatctgatcg
     4741 cactctgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntcc ggcttgacag
     4861 ccatcttgaa tgttatgaaa accaccctgt cagcatcatt gaaagaggtc acctcacaac
     4921 cgcattcagt cctgaaataa tgagggaaca catctttaaa gaaatccgct tttattgaca
     4981 aatttccagg gtgtgtgatt atattcaagg attttttttg aggtggtcat gtttccggca
     5041 ggcaaagcag ttgatgcaag agaatttgat aaaagggcag ttgagcgggg catcccttcg
     5101 ctgatactga tggaaaatgc cgctttttca ctttttcttg aagtttcaag ggtgattgat
     5161 gagtttaaac ctgaaaagat cgtggttttt gcaggcaaag ggggaaacgg cggagacgga
     5221 tttgcacttt taagaatatt gaaagaccgt ggctgcaaca tcccgatgta cattgttccg
     5281 ttttttacag ttacaccgtt acacaattcc acgggcgcga taaatcgtgc ccctgcatta
     5341 accactcacg actcaccatt cacggttcac gactcactca caaactggaa gatgcttcca
     5401 aaatcggttg agataaccgg tatggaaaat atttcaggta aagtgctgtt cattgatgca
     5461 atggtcggaa cgggtttgaa agatgatttg actggcagaa tgagagaagg tgttgaattt
     5521 attaataaat ataacgacaa gtttgttgtg gcagttgaca ttccgacagg gcttaacagc
     5581 gataccggaa ttattatggg tgttgctgtt gttgcagatc ttaccgtcac gatgggaatt
     5641 tataaaacag gtctttttgc ttctaaagga ccgtcggttt caggcagaat agtgcttgga
     5701 aaaatttcag caattgatga tgaaaaagat caatatactt attttgttga agaaaatgtt
     5761 gttccagaac tggttgatat tcccatagac agtttcaaaa ataaaaacgg tcatcttctg
     5821 atcatcggcg gagatcttga caagctcggt gcgacaataa tctccgcaag atcgtttatg
     5881 gtttcaggcg gagggctcgt cacggccgct ttttcaaaag atctgcatga aaaaattgca
     5941 ggggtaatgc cggggatgat gcttgcagac attgatgaag ttccaaaaaa gcttcatcag
     6001 ttcagctcga tcatcatagg tcccggactt tcaaaatggc cttttgaaaa acatgatatt
     6061 tttaaaaatt ataaaggaat tctcatcatt gatgccggaa tgtttgatat aatgaatgaa
     6121 ttcaatgata tacttgaatc tctaaaagat ctgaaagtgg ttttcactcc tcatcccgga
     6181 gaacttaaaa gatttctgaa agctcataca gatgaaccgt ggattaattc tgttgaaaga
     6241 ttccagctta aaaaagacca tattcttgtt gcaaaaagtc attcaacatt tatcagatca
     6301 gttgaaaaaa ccgttataat accccacgga gcgaaagccc tttcttttgg aggaagcggt
     6361 gatgctctca caggtttgat cgcttatgaa actaactttt caggttttga gagcggagta
     6421 aaaagagctg ttttaagaca tcgaatggca gggattgagc ttgaaaaaag atattcagca
     6481 acattccacg atattggcaa acttattgaa cttatcggaa ttacaggaaa aaaagatgtc
     6541 tgttgaagtc cattccgtaa agaacgaatc tgaaaccgca ctgattgcta aaaacattgc
     6601 ttctgaaata aagggaggcg gactgataac tcttgacgga gatctcggtg caggaaaaac
     6661 attttttacc ggtgcgctct gtgaagcact cggattggaa agaaaaagtg tgagcagtcc
     6721 gacattcgta ataatgaaaa aatattcagg aatagtggat gtctaccatt gggattttta
     6781 ccggatatgt tccgttgatg aattatatgt tgctgatttt tttgaatatt tatcagaaac
     6841 aggaagcatt accatagttg aatgggcttc aatgttcaaa gagtgctggg aaaaccactt
     6901 aaaaagaatt gagataaaaa tagagttcgg agaaaaagaa gatgaacgac gaatcgaaat
     6961 caaaagaaat ttctgaaaac agcgacgaaa ctcaggaaga gagtatattt aacagaccca
     7021 gacagaaact ttcaatagcg aaagagagga atcaaaaacc tgaaactccc ccgccaccga
     7081 agcatgaaat ggccatcaaa gaagaagaga aaaaaccgga caatgttgaa atcgttaagg
     7141 aaaaagtatc aaaagccgtt gaagtcacaa aaaagaagat tgatgaaaca ctcaaaggcg
     7201 ggacaagtct taaaactgcc gcaaaagcga tcttttcaaa cggaaagctt actgttgcat
     7261 ccctgacagt tgttctggca atggtgctta tcactatttt ttcatatcat cagatatctt
     7321 catgggtaga ttcgctcaca gctccgttct ttgacaagga accgttgaca gaaggttttt
     7381 ttgattatat ttattacttc ggatggctta tcacacgcat ggtcttcaaa gctgttgcgg
     7441 caatagttgt tttctatatc tcattcattt tttcatacac ggtcacatca ccgctctatt
     7501 catttatttc agtactcgct gaggacattt atttcggcaa acccgaagac gcaactgatt
     7561 tttcacttga tataatgatt gaagatgttc ttcaggcatt gaaaattgca ggaatggctc
     7621 ttgtgtgcac agtttttgcg ttttttgtga actttgtgcc tgtaatagga cagataactg
     7681 ctatcgcatt ctatatagtc ataaattctt taatgctgat cgattttccg gcatcaagaa
     7741 gaagctggga ccttaaaaca aagactctgt ggataaggga gaatctcatg actaccctcc
     7801 gcatagggtc actgcctacg cttatatcaa tgataccgct tgcgaataac ataattcttg
     7861 ctttctgttt tccgttattt gtagtacatg cgacaatgaa ttttgcggaa gttgaaaaga
     7921 acaggacaga taaaagtatc agttaagatc ttccagagcg attcttgatt ttacgaaata
     7981 ctcgtttgcc ggcatgaggg tttttgtcag aagagtaaat atttcccttg ctttgttttt
     8041 gtttgtttct ctcattccgt aagcttccca gtacattgat tttgcagaaa tattaataga
     8101 tttcattcca tcaagagcaa tctgattgtt tttatcaagg aaaagtgcct cagcaaaagc
     8161 tcttctcgct ccgagaagat ctttttcatc aagttttaca agaccgttct tggcaagtgc
     8221 ctcagctttc aaggaagcga tgtccgcctg tgcttttaca ccggtgacct cccttgttct
     8281 tgttatttta tattcttccc tgaatttatt gcttatttca tcaatttcct tctgatagat
     8341 atcttttgtg ctttcttcat atttcgcatc ggcatccttt cttttctgtg atgcttcctt
     8401 cctgtcatct tcgttcattt tcttcatatc cttaacaaac tgatctttgt cgattttgcg
     8461 ctgtttttct tcttcaaccc actgtttttt cttagtatcc cagtttgttt tttcgttttc
     8521 aaggattttt gcccgttcat cctttcttct tttcttttcc tcattaaggg actgtatttc
     8581 ctcttcccat tttattttct gctgcttctg agcttctttt tctgaaacct gtttctgtgc
     8641 atattttaca tcaacagctt ctttcttttc cttatattcg gcttcggttt tttcatattt
     8701 ttccttccac tgggtatcaa acttttcaaa tgcggtctgc tggctggctc tcaggtcctg
     8761 acttgtgttt tcccattttg tgtttaccgc agttatgtcg gctgctattt tttcattgtc
     8821 tttaatgagg cgatcttttg catctctgtc aactttcagt tttctgtcaa gattggcctg
     8881 tcttttctgc ctgtctgaat cccatgaacc cgaaagtttt gttctatctt tctgacggtc
     8941 ttcgatttta cggtctcttt ctctctgggc aagcgctatc tgatcatcta tgctcttttt
     9001 gaacatatct tttcttttct ggaaaccgcc gaaatcacct tcagctttct tcttttctgc
     9061 aagttttgca gttctgaatt tattgttgtc tttctgaagt tcgtcattct ttttggcaag
     9121 ctttgtatct atctccatac tttttgcttc atactccata tcaactttca taacctgatc
     9181 agcaataaaa gtctctttgc cgctttcgtg tgcagatatt ttcttttcaa ctgcggcaag
     9241 ctgtttttta agctgaggag tctctttttc agctatcttt gcttcaagat cagttttttc
     9301 agttgtgagt ttcgcgaggt cttcggtata tttcttttct gcttcagcgg ttgcactctc
     9361 tttttttgtt ttgtattcag catcaacagt tgctctttct ttttcaagct cttcctgaaa
     9421 ttttgacatc agctcaacat tttttgtcat atattcttca atagctttgt catatttttc
     9481 aagatctttt cttttttcag attcctgctg atcgaaatcc tgcagttttt tgttgagttc
     9541 atcaaatttg acctgatatt ccttttcaac ctgaacctta aattttctgt cttcgtcttc
     9601 atttttcatt tttacttctt catttccgcc tgagaatttc ttttcttcag catcgaattt
     9661 ttccatttca gcggctattt tcttatcgat ctcaactatt tttgcatcgt ttgccttttt
     9721 cttttcctca agctttacga cagcggcttc gttagctttg gacccctttt caatctcttc
     9781 cttctgttta ttctcgaact gttctttttc agtacgcatc tttttaagca gttctgtgta
     9841 tttcttatcg actgcgtcca tttcatcttt acgggctttt tcgtcacctt cagtagaaac
     9901 cttcacaaca cctttttctc tctgttcaaa aagagttaat ttgttctcaa gtgccactat
     9961 tttctgttct atttcgctgt catctttttc ccactgatat ttcaagtcct taattttctt
    10021 ggctagatcg gcatattcct tcttttcaag ttcaacagca ttttttgaat ctgtttcaaa
    10081 ctgctttaac ttgctttctc tttcaaggat ccttccgtta agccatttgt cgatttctgc
    10141 aagttttgcc gccttgtctt ttctcgattt ctctgtcaat tttgaatatt ttctgtgtat
    10201 agaatcaacg atctggatat cttttgatgt gggctcaaga taagtaaccc tttcaacact
    10261 gctgtccttt gaatatgcac ggtattttac accgagccca gttatctctt cattggcttt
    10321 ttccgctttg gagatcatca ggactgcctc ttcatggttt ttagctttga ttgcggcatt
    10381 gcctctagcg gtgaacttta ttgcgtcctg cacattgtta aggagttttt ttgcaactac
    10441 atctctggac gcagcttttt caaaatagga ttccgcttcc ttaaggttcc cttccgcgta
    10501 acttgtaaat cccgacatta tgaggttttc aactgaaacg accctggatt ttgacgatct
    10561 gcttgaagca tcttcctctt cttcttcgta atccgaatcc tcgttctcat atttctgttt
    10621 ctttgccgct ttctttgatt tcctttcagt agaaaagtca gaatcagaaa tccctgaaga
    10681 tgaagtatgt ttttttaaaa atctgttcca gtaggttatt atttcatctg cgatcgtgtc
    10741 ggcttcagca aattcatcaa gatcgatctt tgcagattca agacgattat attttctgtc
    10801 aaaaaggtat gtgttgactg tgatctcgct tccgatcttc agtcttgtaa aaaggaaaaa
    10861 atcgaccttt tttgatattt ttcttgagat gttctgctga catttgagat cttcgcggca
    10921 tttgttgagt gcttttctgt catttgtcga gagcagttct ttaaaactga ccggctcagc
    10981 tccggagatc ttgccaaagt tctcaataag aaatcggtca acatcttttg caacggtcca
    11041 ctcgcctcta ttctgtgctc ctacaggaaa gactgcaaca gaatcagcgg catgaactat
    11101 ggacactgca aaaatgaatt ggaaaaggat taatgctctt ttcaaggttt cctccagctt
    11161 ttagctatat gaatcaataa aaaaatgccg tacaaaaaac atgctcctgc gataatgcga
    11221 caatatgtaa ctaaactttc ctctttaagt caaatttttt tattgacaag aacaagttgg
    11281 tttctataat ctccgacacg ctgtgcatgg tctactgtgc cggcgaaaaa aaacaggagg
    11341 tgattttaga atgtcagaat taacaaaaaa aggttttggt gaactgcttg aagagttcct
    11401 tccgagtgaa aaggaagttt cgagcgaaga aatctgcaaa ggtgttgtaa gggcagtgag
    11461 gaaggatttc atcctggtcg atgtgggact taaaagcgac ggcataatcc cgtcatcgga
    11521 gttcactgct gaagagatct caaaactgaa ggtgagcgat gagatcgaag ttgttgttga
    11581 cagatgggaa gacgagaagg gcaacatctg cctttcaaaa gagaaagcag gcaagctgaa
    11641 ggtatgggaa gttgttgaaa aggcatttga cgacggttcg ctcgtaaacg gaactatcgt
    11701 tgcaagagtt aacggcggtc ttgctgttga tatcggtgtg aaagcattcc ttcccaattc
    11761 acaggttgct ctcagaccta taaagaatgt tgatgacctt atcggaaaga attttgattt
    11821 caaggtcatt aagtttgaca gaaagaaagg aaatatcgtt atatcgagga gagatcttct
    11881 cgaaaaggaa agagaagtgc ttaaaaacac aactcttgaa aaacttcagg aaggcgcagt
    11941 ggttaaagga cttgtcaaga acattactta ctacggcgtg ttcgttgatc tcggaggaat
    12001 agacggtctt cttcatatta cagatatgtc atggggcaga gttaaccatc cgtctgaaat
    12061 gttcaagatc ggtgatgaag ttgatgtaaa agttcttaaa tacgacagag attccgaaag
    12121 ggtgagtctt ggcgtaaaac agatatcgga agatccctgg atccacgctt ctgacaaata
    12181 tgcatccggc aaaacagtta agggtgttat cgtatctctc gaagattacg gtgctttcgt
    12241 tgagcttgaa agaggagtcg agggacttat tcacatttct gaaatgagct ggacaagaag
    12301 ggtgaaacat cctggtaaaa tggttgcgat cggagatgaa gtggaagtca agatccttga
    12361 gatcgatgtt gagaacagga gaatttctct tggaatgaag cagatagaac ccaatccatg
    12421 ggaactttta agagaaaagt atccgataaa tactgttgta agaggaacca tccgcaatat
    12481 tactgatttc ggtctttttg tgggtgttga agagggtatt gacggtcttg taagaacaac
    12541 agacatcact tgggacagaa aattgaaaca tccttcagaa tattataaaa agggtgatga
    12601 agttgaagct gtcgtcctga atattgatgc agaaaagcag agattttctc ttggtgtgaa
    12661 acagctttcc gaagatccat ggaaaatgtt caaagaccag cataatattg gtgagaagat
    12721 cgaagtcgaa gttgttaaga tcgcagattt cggtgttttt gtgaaagttt ttgacactat
    12781 cgaaggactt gttcacaaaa ctgaattcga agatgagaac aaggaatatc agccgggtga
    12841 taaagttgaa gtcataatca agtcgatttc tgatacagac agaaaggttt cgctgagcgt
    12901 aaaagctgtt tcagacgaag atgctcaaaa agagatcaga gacgcagccg ctaacgagaa
    12961 agttcctgaa aaactcggtt catttgcaga tgcattcaaa aatgccgagg acgctgaaaa
    13021 gaaatagcag aaatttttgt tgtgaatttt taaagggcac tccggtgccc ttttttttat
    13081 gttatatcgt taatgaatac atcgacaatg tcaggatcaa actgtgttcc gctgaatttt
    13141 ttgagttcac tcaaagctct ctgccaatct ttttttctgt gatatatcct gtttgttgtc
    13201 attgcatcaa aggcatcggc aacacgcatt attcttgatc ctgccggtat catttttgtg
    13261 gaaagacttt cgggatatcc tgatccgtca aaattctcgt gatgatgaag aatgtagtat
    13321 gcgatcttgt ccattcctga tatgctgttt attatatcat agcttctttg agggtgcatt
    13381 cttatcgaca gccactcttc ttctgagagt ttctctttct tcagataaat tgatctgtcc
    13441 cctgaaattt ttcctatgtc gtgaagaaaa gccgcccatc ttatggcaag tatctcttct
    13501 tcatcaagtc ccatgaggta tgctatctgc acagaaagtt cagccactct cattgaatgg
    13561 ttgtcctgcg gtgaagtaag attgtctgta agcattgcga ccattttaaa aaaccggttt
    13621 actgtgtcgg ttgatgaaaa gtaatatctg tcatccatct gttttttaag agacctcatc
    13681 tcgtttttaa tatcatcttc tgtgtttatg aggttcagca gtccgttttc agcaagtttt
    13741 acaaaagtgt tgtaaattac agggtcgaac tcttcacctg aaaccatgtt aaggaaaagt
    13801 atgagttctc cagtgtcatc aattttatga agtcttgaaa aaatatctat cgagtcggca
    13861 acccttatta ttcttgaaag aagagggatc ttttcgcctt ttaatccgtt tggaaagccg
    13921 ctgccgtcaa aaaattcgtg atgtgcgccc actatctcgc tgatctttct gaaagtggga
    13981 aaactcctta agatcgtttc gcccctgtgt gaatgggcaa acacgaaaaa atcgttcttc
    14041 tgtccgaaaa tgtcgggcac ctgaacaagg gattctatta tgtgtccaga tatcattact
    14101 ccgccgatgt catggagaag tgaagcaaaa aaaagttctt tcattctgga cttttcgagt
    14161 ttcagtgcct ctcctatttt gcatgctata atgcagactt tccaactatg ataggagtca
    14221 tgaccttcct tgtctttttc tactgctttg gctagttctg agatgaaatc tgcggcaaaa
    14281 gttgcaggaa aagcaacatc ctgcatggga aggtgtgtaa gtcttgattg cggatgtgtg
    14341 gtagtcaatt aaatagctcc ggtctgggag agaatatcct gccatcttaa aagttttttt
    14401 tctgactcct caacaatgga aatgaaaagt tcaatattgt caaatggttt tagaagaaag
    14461 tcgtttgcac cgttttcaag ggcggaaacg attctgtcaa ttgtgctgta tgcgctcatc
    14521 atgatcacct gggtaagtcc gcttatctct ttaacaatat tcaatactgt tattccatcc
    14581 atattaggca taagcacatc gacaagcgct atgtggacaa cattgttcct cacaaaatca
    14641 acggcatctt ccggcttatt ttttgtgaca acagtgtaac cggtgccttc gaggatcctt
    14701 ttcaggctgg agagtatgtt ctcctcatca tcaagaataa ggatggtaat gttctttctg
    14761 tccatggtct ctctccatat tattaaaagt taaagaaatt cttcactaca tcccttgaat
    14821 ttatcatctt cataattgat ttatagttgt tccagaaata gaccctgtca acgaaatttc
    14881 caaggtcgtt ctcattaagg ttcagtgtgt cgatcatttt tatcagaaca cggaagccgg
    14941 tcttttcaaa aatgtaatct ttcatttcat cacgggggaa agagtgggcg agaactgtca
    15001 gcacgacatc gtttctaaga tatgaaacaa atccggcaag tgttttcgga gcgtattcct
    15061 caatgaaaga tgccccggtt ttcatgatcc tgtttactgt tttctcctct tccttgaaaa
    15121 ggtttaccat cggctcatag agaaggtggt tctcattcag tctgtaatgg aaagcatttc
    15181 cgatatgctc tctgtcaaca attcccgttt caataaggaa gtcgagtgcc ttcttgttgg
    15241 aattgggatt ggtgctgctg agtgttgcga tctttctgcc tgtatagttc tgtttgttga
    15301 caagaatccg cagaatgtca actttagccc ttgatccaag caggtaggaa agaggtaggt
    15361 aagtcatcag gatcctccaa aaagtgaagt taaacgaaca gctctcaaat attataggaa
    15421 acacctttgt tttgtcaaaa aaaataatac aactatgtcc gtatttcaaa ttattttgaa
    15481 acaaagtgcg aaaaaagtga tattataata aaaattgata taatcttacg gaaaatttct
    15541 ttttcaactt gatatcggag gttttgaaaa tgtctgcaaa aaaaagatca gaaaaaagtt
    15601 ttgatccgaa atcgcttctc tacaccaaaa gttcagggtg ggatgggatc agcaaaaacg
    15661 aaatgcagaa agtgtcggat ttttcagatg gttacaaaaa gtttcttgac aatgcaaaga
    15721 ccgaaaggga ggcagtgaga tatattcaga agatcgcatt gtcaaaaggg tacaaggaga
    15781 taacaaaagc aaagccgtca gacagaaaac tttttatcaa ttttgacaat gtttcgtgtg
    15841 ttctggcaga gataaatgac acaactttca ataacggatt tcttatggtg ggcgctcata
    15901 tcgacactcc acggcttgat cttaaaggga accctcttta tgaagctgag gatatggctt
    15961 atctcaagac tcactactac ggcggtatca agaaatatca gtgggttaca agaccgcttg
    16021 cgcttcacgg tgtagttgtt acggaagaag gcgaattgaa agacatcact atcggtgaag
    16081 atgacagcga tcccgttttc acgataaacg atcttcttcc ccatctagca caggaccaga
    16141 tgcagaaaaa tgcaagaacg gtcattgaag gtgaaaatct gaacatattg gtaggttcag
    16201 tgccgtacaa attcaaacat gaaaaagatt ccataaaaac ggcaataatg tcaatacttt
    16261 acggcaaata cgggatcaag gaggaggatt ttgtctctgc tgagcttcag cttgtcccgg
    16321 caggaaaggc aagagatgta ggtcttgaca gaagttttgt cggtgcccac ggtcaggatg
    16381 acagagtttg tgcgtatgcc ggaatggagg cgatatttga ttcaggcaag tcatcaaaaa
    16441 ataagatagc gatgttcttt gacaaggagg agatcgggtc caaagggaat gccggcgcaa
    16501 attcgaacat tcttgaacgg gctgtgaaca aaataatcaa tctttacggc aaagggaact
    16561 ataacacact gataaactct cttgcagaca gtaagtttct gagtgctgat gtcaacgcag
    16621 gcattgatcc tgaatgggcg ggtacaatgg aaaagatgaa tgcggcaaag atcggattcg
    16681 gtgtatgtct tacaaaattc accggaagcg gcggcaaaag caattcaaat gaagcgcatg
    16741 ccgaatttgt agctgaatgc agaaaagctt tcaataaaga caaggtaaag tggcagacag
    16801 cggaacttgg caaaatcgat ctcggcggcg gcggaacgat cgcgatgttc atcgctgaat
    16861 acggaatgga tgtcgttgac tgcggaacag cacttctttc aatgcacagc ccattcgaaa
    16921 ttgcttcaaa agcagacatt taccacactt acgcggcata caaagcattc tataaatatc
    16981 tgtaaaccgg ctttatatat tcttatccaa tttcttgcat atttaatact attgctgcat
    17041 tatctatcgt tgactataat tttggagtcg gaaaatgaca ggaaatgaac ttatacagat
    17101 tgatgagatt aaaagaagga ttttcacaat tcggggaatg caagtgatgc tggacagtga
    17161 tcttgctgag ttgtatggag ttgaaacgaa acaccttaac agggctgtga aaaggaatat
    17221 agagcgattc ccggaatctt ttatgttcca gcttgctgaa acggagaact tgaggttcca
    17281 aaatggcacc tcaagacaac atggagggag gagatatctt cctaatgctt ttaccgagtc
    17341 aggcattgcg atgctttctg ctgttttgaa aagtgatgta gcaataaaag taagtgttca
    17401 aataatggaa gcttttgtcg caatgagaaa atttattaat caaaatgcgg aggttttttt
    17461 ccaaatcaat tccgttgaga aaaagttagt agattataaa acagaaacag ataaaaagct
    17521 ctcatttatt ttcaagacca tcgaggcaaa tgagcttcct gccaaacaag gcatcttttt
    17581 tgaaggtcaa atttttgaag cacacaaatt cgtttcagat attgtaaggt cggctgaaaa
    17641 atccattata ctgattgaca attatgttga tgatacagtc ctcacacttt tttcaaaacg
    17701 aaaacgggga gttgcgctta aaattcttac aaaaaatctt tcaaaacagc ttcaactcga
    17761 cactcagaaa ttcaatgaac agttcccgcc cgctgaaata aaggaattca gttattccca
    17821 tgacagattc atgataattg atgattgcga cctttatcat tttggtgcat cgctcaaaga
    17881 ccttggcaga aaatggttcg gattttccaa aatggatgtt gaagttgcga agat
//
LOCUS       DAOOYB010000026        17788 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_3061_length_17788_cov_9.297017, whole genome shotgun sequence.
ACCESSION   DAOOYB010000026 DAOOYB010000000
VERSION     DAOOYB010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 17788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17788
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3061_length_17788_cov_9.297017"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1236)
                     /gene="dnaK"
                     /locus_tag="PL056_02695"
     CDS             complement(<1..1236)
                     /gene="dnaK"
                     /locus_tag="PL056_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HPM45957.1"
                     /translation="MGKVIGIDLGTTNSCVSIMEGGEPKVIANAEGSRTTPSIVAFTD
                     KGERLVGQLAKRQAITNSKRTVYAIKRLIGRKYEANEVMKMKTVVPFQISKASNGDAW
                     VKIDDKDYSPQQISAMVLQKMKETAEEYLGEKVTDAVITVPAYFNDSQRQATKDAGKI
                     AGLNVLRIINEPTAAALAYGIDKKGKEMKIAVYDLGGGTFDISILELGDGVFEVLSTN
                     GDTFLGGEDFDQRIIEFLAAEFKKDSGIDLSQDPMALQRLKEAAEKAKHELSSTMETN
                     INLPFITADANGPKHLNINMSRARLESLVEDLAKKTLEPCRKVLADAKLSVNDINEVI
                     MVGGMTRMPLIQRLVKEFFQKDIHKGVNPDEVVAVGAAIQGGVLKGDVKDVLLLDVTP
                     LSLGIETLGGVTSRRRTSFT"
     gene            complement(1256..2032)
                     /gene="grpE"
                     /locus_tag="PL056_02700"
     CDS             complement(1256..2032)
                     /gene="grpE"
                     /locus_tag="PL056_02700"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HPM45958.1"
                     /translation="MNGNDKDLKKDAVENETVIKEDGTLEIDEKALQEETDLQKLAEQ
                     LGNEHAKTKEKRFFGRKNRDGESSKKIEQLTEQLKEREEKIKELDNTVKMLVAENRNQ
                     KNRIENEFRTKIRFAAEDFFRDFITVKDDFDKAMEFVPNDENAQNDPFIQGIRHLLSK
                     TESVMKKHGIECFSGIGEKFDPSIHQAMSMIPVEGKEQNDIVAEYVKGYRMHERILRP
                     AMVIVASGGSPSTEAEAGVKEEQNEEEPADDKADDDNIEQ"
     gene            2230..2305
                     /locus_tag="PL056_02705"
     tRNA            2230..2305
                     /locus_tag="PL056_02705"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2263..2265,aa:Ala,seq:ggc)
     gene            2382..4178
                     /locus_tag="PL056_02710"
     CDS             2382..4178
                     /locus_tag="PL056_02710"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HPM45959.1"
                     /translation="MKKTLSAIIIFLTFTVFSVDMKSVHMVELDNHRFDKPLISHGKP
                     STKLLNEVALYKPLTEKLIFGYLPYWNNSTDHLRYGLLTDILYFSCELSTAGSLGDCR
                     DWPESAPIDEAHKYGVRVHLVITGFDGTIVQNLVENSDAKNTFFKNCWDKVNDAGADG
                     INIDFEAFADTKTADLIKFFNDLGTYFHSRNPEMIVSAALPAVDWSNRWDIASMTGLD
                     HAFLMLYDYHWKSGEPGPVAPLHSETPWTANGICVEKSINTYISKNGNEVKDKLIAGY
                     PYYGIKWKTTSGSFPGAKTENGAAVIFDNVFKDHSSIESGWDSGSETPYKIWQDGSQW
                     YQLWYDDDESISLKYAFANSKGVAGSGMWALNYDVSSEDLWKKIAENFVSDRSGSFEN
                     PVKIDSFPFERSDNTYRYASDIVDSYNCSQNISDTTLIDESGPEIIFEFVTECSGILT
                     ATITDGSGGTGSREDVDIHILNGNDGNSCIFRNDSEIKIDIKKGKYYLVADSFSKEGV
                     IKGGNFTILADFICDETENSDEDDTKADDDVVEISDGIEIPDEESTENDDETAIVESD
                     NDNEEADDEFEEKADHDSENTKKQSGCAINAI"
     gene            4168..>4525
                     /locus_tag="PL056_02715"
     CDS             4168..>4525
                     /locus_tag="PL056_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45960.1"
                     /translation="MRYDMLKKSFFAFIFTLILSSCSNEIHREGKLFFYRSEGTKLFT
                     LYQNDDQNYLLSTKLSPLRSDWEKKISGHPFEPAFFIFDNYILCNCKKGKVCLLDSSD
                     GNVAVSVDSSAVITENS"
     assembly_gap    4526..4625
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4626..5195
                     /locus_tag="PL056_02720"
     CDS             <4626..5195
                     /locus_tag="PL056_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45961.1"
                     /translation="GTTFLTCQESLSLAAFTVKNLEDEAQTEEDAVDYFDKVYIFNAS
                     TGERIFDYNSSENLRVLNMTGFMTDRFYLALEEKNEGYDEITVKMFSSTDFKEGLNYV
                     FGNDSTSEEVYISWMHTDINYTVFRISTITGTSVETNFLFRTDTAEKLGRMESYPEIV
                     TGTNAYDIISYDDYFNVVEKTLPDFLIYD"
     gene            5214..5780
                     /locus_tag="PL056_02725"
     CDS             5214..5780
                     /locus_tag="PL056_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45962.1"
                     /translation="MKKIFFIFATLFFTFAMFAQEPEQPDAVNRNVPGEVHNWQLNFG
                     TNFVGVTHFIDIGTSTNIGLPFTATSLSAGYQLSNQIWLMAKFHLYMSMFDDDASAEF
                     LIGPGIKAEFVRTDHISFYGGIFASIGNSGKTFLFSPELFIGIDYNVKNYLGLGILTD
                     FGYLLYARDGNTQHCINFIIGPNLTIYF"
     gene            complement(5784..8693)
                     /locus_tag="PL056_02730"
     CDS             complement(5784..8693)
                     /locus_tag="PL056_02730"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HPM45963.1"
                     /translation="MKIRIILLTTLFAMLFGFSCSSSKKNKNDEEFMDDKVNDEDVLK
                     DEFGFDLSGYNPELKKFSALVKTSRMSGVSTTEFNAEVFITGGNVEGDATYEWTLPAE
                     GKFAAGSKKTDKKIVVTFSKPGRHTVTVSVKDGSGEVVETGGVLMVFDSTHAYTVGDL
                     NSDKVVDDKDVTLLKDYLERKNTLTDEQIKGSDFNLDNRIDSNDLAILETALDSGKAP
                     VFIQGGVKESGDPIMSGDPIMSGDPIMFTASGDPIMIINPELLEPGVESTIAITPSGT
                     LRAVKWDTACLAEMEKHAESEKVIFDSVRPGYATFVIPPKYICLTAEGTFDVVLKKGE
                     KEISLIKDMTIRPLPVAEKVPGSKVVETMGRLRVAIEAMEGGVTEYAENIDNINSDEK
                     AVMIGMIKAASELYNDAYGNFYSVFNRLDDQTKELFERYAKAKGLDSLNDTLKTATEE
                     VIYYTNALKNDSSYLKAGFGTPAIGEDMIKLVCAFAQIADAAQAVADINDDVTSVLEY
                     LDWWPLNRAPIIGPAVDFLNALTNVVGAVTDIIGMVAEYVPTFGDIKVEVSSPQMGIG
                     GSTKIDASIKVNIVSGLCNKAGGALVDSIMDKLKDALTKRMGSMIPVAGKEFKNAKYE
                     VEQMGTIAAGIYKAVGWIAGKIVDATGLNDYMQKLADKFCEMFKDPFMAISADFLAAS
                     CGAIEFDGGVGTWPCTEECAGDPPKTITYSGDRKVCDDEKNGAATATCYKGNVDDDNI
                     DDNDYENDYDIEQPDTDIEMTVTQELVENITTGTETPDVTITKRTETVPPENTGQEYF
                     SIYFNSFLDGGETTPPALKKTVVLRGLGEPLYASGFPGMNVIFYLDWNEYFEVVTTDN
                     APAGFVIKGSGWNGWYVFDKPEIQIFGTTGNQGSIWVNIVLPDDPKYLGTHEFEIAFL
                     NQKSDGTPLLSAAGPYSTFSLKLLEKDGYVSCHSDLECDTETQVCDVSYYYCVPK"
     gene            complement(8705..12274)
                     /locus_tag="PL056_02735"
     CDS             complement(8705..12274)
                     /locus_tag="PL056_02735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM45964.1"
                     /translation="MIYYTANQLDYLIFILGAFIIVEVLKFFISRFFSLRYERKIICV
                     KKDFYISFLVILVLGWLSAEYKGSLADSNLKKDILFQAEAVASTINGDYVEKLLFTQE
                     DISNSHFQRIRSQMISYGKYLGNLRGIYSMAEKEGNIVFGPEDYDESDPMASMPGEIY
                     KMPDDPLRQSFADGIPRVAGPFTDEYGTFVSAYAPVFSRKSGEIILLIGIDVLADDWQ
                     KQIHIARAASMLRTMLVVLIFFTFFSMMGIRDSTKKKKRWFFKHLEAELIFIMGFLLA
                     LVFGMNASKLVQISERDDSMNLAQAQAQLVRHEFFDVRKNIEAMAGYIGTIESGDKIF
                     EKLAAPVVRSAIVSYLQWVELNDDGRAFPVHGMSDNGFVIEKNKWVSSPYFYQNFAGA
                     VKSRMSVSIQNFNEKGEMEWVVITPVYSNLTDRAKGALITAVDTDFLINCTYPLVKFS
                     DGNSHLHLKVLSESGTAEPLVSIADNDSECNDPSRVISGPLFIFGSALSIDMIQKPQP
                     LFSTSLIIGMITFILIFLLCILFSFFIAGLRNRESTLEVLVRNRTKELSESNERFMLA
                     VTGSHDGIWDWNIRTGELYLSPRWKGMIGYEDHEIPNILDSFERRIHPDDKIRVMHFI
                     EDYFKGHEKKYEIEFRFRHKNGSYVWILARGDGLRDENGIVYRMAGSHTDITERKKVE
                     ETILSAKKEAESANNAKSEFLANMSHEIRTPLNGIVGFSDLLIDTELSDIQKQYMSNV
                     SKSAHILLEVVNDILDFSKIEAGKLELDYHAVNISALLNEVAGLTSHQIIEKPIDLEV
                     KLSPDIPEYVRTDQFRLRQVLINLLSNAAKFTDKGRIVLSAVLREKNEEKNKVKVLFS
                     VNDTGIGIKEDQLQNLFESFTQADPSTARKYGGTGLGLAISNRILEKMGSGIKVISEF
                     GSGSEFCFELDFEVSADEKTVTAINADNCDNEKIKLQESSFRMLVVEDNQINMMLIKA
                     IFSSIMPKCDVMQAENGDQALTMFTEEHPDIIFMDVQLPGKDGREITSEIRVLEKNLQ
                     KRTPVIALTAGAVEGEKEKCLAAGMDDFISKPVEVNRIVQILCRWLIYAGSDNHFEKS
                     IFIQNIGGDIPLYKKLLEFVKEDFPIKIKQARTSVAENDCAKLASIAHTLKGSSLNMR
                     FTKLAEFAVMLEKTCKNGCDTDRLNAILDEMEIEFKYLIDNEF"
     gene            complement(12353..14062)
                     /locus_tag="PL056_02740"
     CDS             complement(12353..14062)
                     /locus_tag="PL056_02740"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPM45965.1"
                     /translation="MKKLSVVIAFTLLFSAIYAEEPCYYYGEKLTCLEKIRMDFSVES
                     NSYVKSVSIDGGLKLPLTGEIAVVFRKGVSKKVIAGIIEKYSFKIARTLDGIEGYFIF
                     KTPENTELFNVSRELYESGFVKLAQPVWLERPVLAFVPNDTYYGDQWQHKVINSETAW
                     DKAKGYPDAKIAVLDSGVDTMHPDLFLQLGMSFVPGEPQIDPNMGALQNKYYMAHGTA
                     VSGIAAGQGDNGIGVSGVCPKCSVIPVKYIGLEMSNPPADRKLNAVKWAVDAGAWVIN
                     NSWTIAPDTESQTDQCIDIPLDNFVKEMTDYAVNTGRNGLGTIIVWAAGNSACDTVRN
                     PSLLDDRNVVVSGLDVDSTLVYYSNYGINVDIAAPAGDADPAKSGLITTDVTANGKGF
                     NPAFNDANSGYTDYMDQAYTKYFDGTSAAAPVVAGALALMLSVNPSMTAPEAISCMKK
                     AASAPDAECSHGEKSVCFGAGIVDAGKMIELASSGECGGEPVEFEDDEETPDETVDES
                     VEEHENDVETESPDSNGNTETPDVEKRVDEEERSDSSATDDKPDNANVPEDESTGCLV
                     LTI"
     gene            complement(14179..15459)
                     /gene="hisS"
                     /locus_tag="PL056_02745"
     CDS             complement(14179..15459)
                     /gene="hisS"
                     /locus_tag="PL056_02745"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HPM45966.1"
                     /translation="MISAVKGMNDLFGRRAMRWGRMEEDIRRIVENANFTEFRTPILE
                     EIALFKRGVGETTDVVQKEMYDFLDKKGRHVAMRPEGTASVVRAFIEHGIAMNEPMPF
                     KLYYIGPFFRYERPQKGRYRQFHQLGIEIFGDETPQMDAEMIFTACRVLEHFKIDYEV
                     HINSIGCKECRPAYREKLVEYFSSKKEHLCEDCQKRIDTNPLRLLDCKTCNPKAKYHD
                     IPVMTDNLCGSCDTHFKQLKDALEIFHVNGIIDPFIVRGLDYYTRTAFEIVANTGGSQ
                     NAVAGGGRYDNLVSDIGGPDTPATGFAMGLERLFDLISEDFYTEPLISAGYALCDEAV
                     DDLVHFAKHMSHHPVRFFADYKPKKFKKALQKADKAGAEFMFIIGEDEAKDKKILMKN
                     LNTGEQKLFGQHEVILIVNELLNYESGKFSATKV"
     gene            complement(15459..16058)
                     /gene="tmk"
                     /locus_tag="PL056_02750"
     CDS             complement(15459..16058)
                     /gene="tmk"
                     /locus_tag="PL056_02750"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HPM45967.1"
                     /translation="MFIAVEGIDGSGKSTTIKELSRYLLSKGKDVLLTAEPTDMSSGK
                     IIREVLSRKDSDSPLTHEMLALMFAADRLNHLKERIWPALKAKRTVITDRYFFSSVAY
                     QSVNVSYEWVKGVNRFATMPDILVFIDVSIDKALERLSIFRHSTELYETREYLEQIKA
                     NYETVISDFSEHVKVVRLNGNLKMDEIYSDIETKFEGVL"
     gene            complement(16061..16678)
                     /locus_tag="PL056_02755"
     CDS             complement(16061..16678)
                     /locus_tag="PL056_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45968.1"
                     /translation="MKERVFFLFSAVFLLISCSEAKLEKIFEDNFERAELGENYTVQG
                     GDWKIEEGAITSSKAENRNLVLTGAALPQNGVIELTMWSESEGVDVKFNGWGDGKIHD
                     HGDGYSFILGGWQNRVSVISKLHEHEKKRSEERDVRLKKGERYRVRIVRKGSDIKWFV
                     NDRHFMSYNDDDPLKTSDGFDRFSFGNWRSKVFFDDLKIYKFAGE"
     gene            16951..>17788
                     /locus_tag="PL056_02760"
     CDS             16951..>17788
                     /locus_tag="PL056_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45969.1"
                     /translation="MKKILSALMFFGFCMFLYSDSLNESSDPDDNWVSISEHQGVGWN
                     GLTSVKSIHHYEDYIYVGGEFSAVKSTLANSVARLHLPTGIWSALGSGLNGGVVSLSI
                     VNSEKIFAAGQFQLEDDDHYYDGVYMWNGENWEVISLDFDGDIFSTAIDKNGNLYVAG
                     EFRNIGDVEVNNVAKWDGKKWSSLGNGSNYEVTNIMFDKEGNLLANGGFLSSDEKSVY
                     YFAKWDGQNWISQETGSLKKVSAFTIGKDGYLYLIGFFDESYIGKIVKWDGNDWTVLN
                     ENS"
BASE COUNT         4906 a         4033 c         3268 g         5481 t
ORIGIN      
        1 tgtaaaggat gtcctccttc tcgatgtaac accaccgaga gtttcaatac ccagtgaaag
       61 cggtgttaca tcgagaagga ggacatcctt tacatcaccc ttgaggactc cgccctgtat
      121 tgcggctcct actgcaacta cttcgtccgg attgacacct ttgtgaatgt ccttctgaaa
      181 aaactctttc acaagacgct gaatgagagg cattcttgtc atgccgccca ccattatcac
      241 ctcattaata tcattgactg aaagttttgc atctgcaaga accttcctgc atggttcgag
      301 agtcttcttt gcaagatctt caaccagcga ttcaagacgg gctctggaca tatttatatt
      361 gaggtgtttc ggaccgtttg catcagcagt aatgaacgga aggtttatgt tggtttccat
      421 cgttgaagaa agctcgtgct ttgccttttc ggccgcctct ttcaatcttt gaagagccat
      481 cgggtcctgt gaaaggtcta tacctgaatc ctttttgaac tctgccgcaa gaaattcgat
      541 tattctctgg tcgaaatcct ctccgccgag aaatgtatca ccgtttgtgg aaagaacttc
      601 aaaaacaccg tcaccgagct caagaatgga aatatcgaaa gttccgccgc caaggtcata
      661 aactgcgatc ttcatttcct tccctttctt atcgataccg tatgcaagag cggctgcagt
      721 cggctcgttt atgatcctga gaacattgag tcctgctatc ttcccggcat cttttgttgc
      781 ctgacgctgt gaatcgttga aatatgcagg aactgtaatg accgcgtcag taactttttc
      841 gcccagatat tcttctgcag tttctttcat tttctgaaga accattgcgg agatctgctg
      901 aggactgtaa tccttatcgt cgatcttgac ccacgcatct ccgtttgatg cctttgagat
      961 ctggaaagga acgaccgttt tcattttcat gacttcgttc gcttcatact ttcttcctat
     1021 gagccgcttt attgcatata ctgtcctttt tgaattggtt attgcctgtc tttttgcaag
     1081 ctgtcccaca agccgctctc ctttgtcagt gaaagcaact atgctcggag tggtccttga
     1141 accttccgca tttgcaatta ctttcggctc accgccttcc atgatgctta cacaactgtt
     1201 agtcgtacca agatcaatac cgataacttt tcccattttc ttctcctact ataaattact
     1261 gttcaatatt atcatcatct gctttatcat cagcaggttc ttcttcattt tgttcttctt
     1321 taacaccggc ttctgcttcc gtcgaaggac ttccgcctga agcaacgata accattgcgg
     1381 gcctgagtat ccgctcatgc atcctgtaac ctttgacata ttcggcaacg atgtcattct
     1441 gttcctttcc ctcaacgggg atcatgctca tcgcctgatg tatcgaggga tcgaattttt
     1501 caccgatgcc tgaaaagcat tcaatcccgt gttttttcat aacgctctct gtcttggaca
     1561 gaaggtgcct tatcccctga ataaaaggat cgttctgagc attttcgtcg tttggaacaa
     1621 attccatagc tttgtcaaaa tcatctttca ccgttataaa atccctgaaa aaatcttctg
     1681 cggcaaacct gatctttgtc ctgaattcgt tctcaatacg gtttttctga tttctgttct
     1741 ctgcaacgag catttttaca gtgttatcca gctctttaat cttctcttcc ctttctttca
     1801 gctgctcggt caactgctca atttttttag atgattcacc atccctgttc tttcttccga
     1861 aaaaccgttt ctcctttgtt ttcgcgtgct catttccgag ctgctcagca agtttctgca
     1921 gatcggtctc ttcctgaagt gccttttcat caatttcaag tgtaccgtcc tctttaatta
     1981 ccgtctcatt ttcaaccgca tcttttttaa gatctttgtc gtttccgttc atctctcctc
     2041 cgacggactg atcaccttga cgcaagtgcc tgtaatcatt aagcaccccg atcaagacca
     2101 taataaacgc ccttttatat aaagacatat ccgttaaagt caactgcttt catatataat
     2161 tttttcctca cttttcattg actcgaaaaa caaaacaggt aaattacaaa agccttttga
     2221 tctcatggtg gggctgtagc tcagttggga gagtatttga atggcattca aagggtcgcg
     2281 cgttcgatcc gcgtcagctc caccagtttt cgttgacttt tcattatata tccgtatgaa
     2341 ttgacttggt ttttgtgatt ttttctatgt gggttccaat tatgaaaaaa actctttcag
     2401 cgataatcat ttttcttact tttactgttt tctctgttga tatgaaaagt gtccacatgg
     2461 ttgagcttga caaccacaga tttgacaaac cattgatttc acacggcaaa ccgtcaacga
     2521 agctattgaa tgaagtcgct ttgtataaac cgctgactga aaaacttatc ttcggatatc
     2581 ttccttactg gaacaactcc acagatcact taagatatgg acttctgacc gatattctct
     2641 atttttcgtg cgagctttca accgcaggat ctcttggcga ctgccgcgac tggcctgaat
     2701 cagcaccaat tgatgaagca cataaatacg gagtaagagt tcatcttgtc ataaccggtt
     2761 ttgacggaac gattgtgcag aatttagttg aaaacagtga tgctaaaaac acatttttca
     2821 aaaactgctg ggataaagtg aacgatgcgg gagctgacgg aataaacatt gactttgaag
     2881 cattcgcaga cacaaaaaca gctgatctca ttaagttttt caatgatctt ggaacctatt
     2941 tccactcaag aaaccctgaa atgatagttt cagcggcact tccggcagtt gactggtcaa
     3001 atagatggga tatcgcttcc atgaccggac ttgatcatgc gtttcttatg ctttatgact
     3061 accactggaa aagcggagaa ccgggacctg tcgcaccctt acattcagaa actccctgga
     3121 cagcaaatgg aatatgtgtg gaaaaatcaa taaatacata catttccaag aacggtaacg
     3181 aggtcaagga caaattgata gcaggatatc catactacgg tatcaaatgg aaaacaactt
     3241 ccggctcgtt tcccggagca aagaccgaaa acggtgcggc agttattttt gacaatgttt
     3301 ttaaagacca ttcatcaatt gaaagcggct gggacagcgg atctgaaacc ccgtacaaga
     3361 tctggcagga cggctcgcag tggtatcagt tatggtatga tgacgatgaa agcatttcgc
     3421 ttaaatatgc ttttgcaaac tcaaaaggag tcgccggaag cggaatgtgg gcactgaatt
     3481 atgatgtatc ttcagaagat ctttggaaaa agatcgctga aaactttgtt tcagacaggt
     3541 ctggctcctt tgaaaaccct gttaaaatag acagcttccc ttttgaaaga tcagacaaca
     3601 cataccgata tgcttctgat attgttgact cttacaactg ctctcaaaat atttcagaca
     3661 cgactttaat tgatgaatca gggcctgaaa taatttttga gtttgtaaca gaatgcagtg
     3721 gaattttgac agccacgatc accgacggct caggtggaac cggttcaaga gaggatgttg
     3781 acattcacat tttaaacgga aatgacggta attcatgcat tttcaggaat gattctgaaa
     3841 ttaagatcga catcaaaaaa ggaaaatatt atcttgtcgc cgattctttc tcaaaagaag
     3901 gtgtaataaa gggtggaaat tttaccatcc ttgcagactt tatttgtgat gaaacagaaa
     3961 atagtgatga agatgacact aaagctgatg acgatgtggt tgaaatttct gacggaattg
     4021 aaattcctga cgaagagtca acagaaaatg atgatgaaac cgcgattgtt gagagtgaca
     4081 atgataatga agaagcagat gatgaatttg aggaaaaagc tgatcatgac agcgaaaaca
     4141 caaaaaaaca gagcggatgt gctatcaatg cgatatgaca tgctgaaaaa atcttttttt
     4201 gcattcattt tcacactcat tctttcgagc tgcagtaacg aaattcaccg tgagggaaaa
     4261 ttatttttct acagatctga agggacaaaa cttttcacat tataccagaa cgatgaccag
     4321 aactaccttc tttcaacgaa actctcccct ttaaggagcg actgggaaaa aaagatttca
     4381 gggcaccctt ttgaacccgc atttttcata tttgataatt atattctctg caactgtaaa
     4441 aaggggaaag tgtgtcttct tgacagctcc gacggaaatg ttgccgtttc ggttgattct
     4501 tcagcggtca ttacagaaaa ttcaannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnggaac gacattcctt acatgtcagg aatcactttc acttgcagcg ttcacagtaa
     4681 aaaaccttga ggatgaggcc cagaccgagg aagatgcagt agattatttt gacaaggtat
     4741 acatattcaa tgcctcaaca ggtgaaagga tttttgacta caactcatca gaaaatctcc
     4801 gtgtacttaa catgaccgga ttcatgacag accggttcta ccttgctctt gaagaaaaga
     4861 atgaaggtta tgatgaaata acggtaaaaa tgttctcgtc aaccgatttc aaggaaggac
     4921 tgaactatgt ttttggcaat gacagcacaa gtgaggaagt ttatatctcc tggatgcata
     4981 ccgacataaa ttacacagtt ttcagaatct ctaccatcac tggaacatct gtggaaacca
     5041 attttctttt ccggacagat accgctgaaa agcttggcag aatggaaagt tatcccgaaa
     5101 ttgtaacagg gacaaatgcc tatgacatca tttcatatga cgattatttt aatgttgttg
     5161 aaaagacgct acctgacttt ttgatctatg attaaactgc ttggagatgt accatgaaaa
     5221 aaatcttttt tattttcgca accctttttt ttacttttgc catgtttgca caggaacctg
     5281 agcagcccga tgccgtaaac cgtaatgttc ccggagaagt acataactgg cagctcaatt
     5341 tcgggaccaa ctttgtcgga gttactcact ttattgatat cggaaccagc accaacatag
     5401 gacttccttt taccgcaacc tcactttccg ccggatatca actttcaaat cagatttggt
     5461 taatggcaaa atttcatctt tacatgtcaa tgttcgatga tgatgcttca gctgagtttt
     5521 taataggtcc cggaattaaa gctgaatttg taagaactga ccacatctct ttttatggcg
     5581 ggatctttgc ttcaatcggc aattcaggca aaacattcct tttctcgcct gaactgttta
     5641 tcggtataga ctacaatgtg aaaaattatc tgggattggg cattctgaca gattttggat
     5701 atttacttta tgcaagggac ggtaatactc aacattgcat aaatttcatc atcggaccga
     5761 acctgactat ttatttctga tatttatttc ggcacacagt agtaataact gacatcacac
     5821 acctgtgttt cggtatcaca ttcaagatca ctgtgacaac tcacataacc atctttttca
     5881 agaagtttaa gtgagaaagt tgagtatggc cctgctgcac tcagcaaagg tgttccgtca
     5941 gacttctgat taagaaaagc tatctcaaac tcatgtgttc caagatattt cgggtcatca
     6001 ggaagaacga tgttcaccca gatggaaccc tgattaccgg tggtgccgaa tatctgtatt
     6061 tcaggtttgt caaaaacata ccagccgttc catccggaac ccttaatgac aaatcccgcc
     6121 ggagcattat cggtagtcac cacctcgaaa tattcattcc agtcaaggta aaatatgaca
     6181 ttcattcccg ggaatcctga tgcataaagc ggctctccca gaccccgcaa cacaacggtc
     6241 ttctttaaag caggcggcgt tgtttctcca ccgtcaagga aggagttgaa ataaatgctg
     6301 aaatattcct gaccggtatt ttcaggcgga acggtttcag ttctttttgt tattgtcaca
     6361 tcaggtgttt cggtgccggt tgtgatgttt tcaacaagtt cctgagtcac agtcatttca
     6421 atatcagtat ccggctgttc aatgtcataa tcattttcat aatcgttatc atcaatattg
     6481 tcatcgtcca catttccttt ataacatgtt gccgttgcag ctccgttctt ctcatcgtca
     6541 caaacctttc tgtctcccga atatgttatt gttttcgggg gatcgccggc acattcctct
     6601 gtgcatggcc atgtcccgac gccaccgtca aattcaatgg cgccgcagga tgcagcaaga
     6661 aaatcagcag atattgccat aaacggatct ttgaacatct cacagaattt atctgcgagt
     6721 ttctgcatat aatcattcag ccctgttgca tcaactattt tgcctgctat ccatccgacc
     6781 gccttataaa tcccggcggc tatggtgccc atttgctcaa cttcatattt tgcattttta
     6841 aattcctttc ccgcaacagg gatcatgctt cccattcttt tcgtcagggc gtctttcaat
     6901 ttgtccatta ttgaatcaac gagagctccc cccgccttgt tgcaaagccc gcttacaata
     6961 tttactttta ttgatgcatc tatctttgtg gaacctccaa tacccatttg cggacttgac
     7021 acttccacct ttatatctcc aaaggtcgga acatattcag caaccattcc gatgatatct
     7081 gtcacagctc ccacaacatt tgtaagagca ttcagaaaat caactgccgg accaattatc
     7141 ggagctctgt tgagcggcca ccagtcaaga tattcaagaa cagatgttac atcgtcatta
     7201 atgtcagcaa cggcctgggc ggcatccgct atctgtgcaa atgcacaaac cagttttatc
     7261 atgtcctctc ctattgcagg agtcccgaaa ccggctttca aatatgaaga atcgttttta
     7321 agtgcgttag tgtaataaat aacctcttcg gttgccgttt tcagcgtgtc gttaagcgaa
     7381 tcaagccctt tggctttcgc atatctttca aaaagctcct tcgtctgatc atccagccgg
     7441 ttgaataccg agtagaaatt tccgtatgca tcgttataga gctcagatgc cgctttgatc
     7501 attccaatca taacggcttt ttcatcgcta ttgatattgt ctatgttttc agcatactcc
     7561 gtgactccgc cctccattgc ttcaattgcg actctgagcc gccccattgt ttcaacgact
     7621 tttgagcccg gcactttttc agctacagga agaggtctga tggtcatatc ctttatcaga
     7681 gaaatctcct tttcaccctt tttaagcact acatcaaatg tcccttcagc agtaagacag
     7741 atatatttcg gcggtatgac aaatgttgca tatcccggtc gcaccgaatc aaagatgact
     7801 ttttcagatt cagcatgttt ttccatttca gcaagacagg ccgtatccca tttaactgct
     7861 ctcaaagtac ctgaaggtgt aattgctatt gtcgattcaa cacccggctc aagcagttca
     7921 ggatttatta tcattatcgg atcacccgaa gcagtaaaca ttatgggatc tcccgacatg
     7981 atcgggtcgc ctgacattat cgggtcgccg gattctttca ctcctccctg gatgaaaacc
     8041 ggagctttgc ctgaatcgag tgctgtttca agtattgcaa gatcgtttga gtcgatcctg
     8101 ttatcaagat tgaaatcaga tccttttatc tgttcgtctg tcagagtatt ttttctttca
     8161 agataatctt tgaggagagt gacatcctta tcatcgacca ccttatctga attcaggtct
     8221 cccaccgtat aagcatgagt gctgtcaaac accatgagaa ctccgccggt ctctacaact
     8281 tcgccggatc catcttttac agagacagtg acagtgtgcc tgcctggttt tgaaaaagtt
     8341 acaacgattt ttttgtctgt cttttttgaa cctgccgcaa acttcccttc tgccggaaga
     8401 gtccattcat aagttgcatc accttcaaca tttccgccgg taataaaaac ttcagcattg
     8461 aactctgtcg ttgaaacacc actcatcctt gaagttttaa caagtgctga aaactttttc
     8521 agttccgggt tgtatccgct cagatcgaat ccgaactcat ctttcagcac atcctcatca
     8581 ttgactttgt catccatgaa ctcttcatcg ttcttattct ttttggatga actgcatgaa
     8641 aaaccaaata acattgcaaa caatgtcgtc agtaaaatga ttctgatctt cattctaata
     8701 ccccctaaaa ttcgttatca atcaagtatt tgaactctat ttccatttca tccagtattg
     8761 cattcaacct gtctgtgtca cacccgtttt tacaggtttt ctcaagcatt acagcaaact
     8821 ctgcaagttt tgtgaaccgc atattaagcg atgaaccttt caatgtatgt gcaatagaag
     8881 caagttttgc acaatcgttt tcagcaacac tcgttcttgc ctgtttaatc tttatgggaa
     8941 aatcctcctt tacgaactcg agaagttttt tataaagagg aatgtccccc cctatattct
     9001 gaataaagat actcttttca aaatggttgt cggatcccgc ataaatcaac cacctgcaca
     9061 gtatctgaac gatcctgttc acttcgaccg gttttgaaat gaaatcatcc atcccggcag
     9121 caagacattt ttccttttca ccctcaacag caccggcagt aagtgcgatc acaggagttc
     9181 ttttctgcag gttcttttca agcactctga tctctgaagt tatctctctc ccatccttgc
     9241 cgggaagttg aacatccatg aaaatgatat ccggatgttc ctctgtaaac atagtaagcg
     9301 cctgatctcc gttctccgcc tgcattacat cacatttggg cataattgat gaaaatatcg
     9361 cctttatcag catcatattt atctggttat cctcaaccac gagcattctg aatgatgatt
     9421 cctgcagttt aatcttttca ttatcacagt tatccgcatt aatagcggta acggtctttt
     9481 catcggcaga aacctcaaaa tcaagttcga aacagaactc cgaccctgat ccgaattcac
     9541 ttatgacctt tatgccgctt cccatttttt caagtatcct gttcgatatt gcaagcccca
     9601 gacctgtgcc gccgtacttc cttgcagttg agggatctgc ctgagtaaac gattcaaaaa
     9661 gattctgcaa ctgatcttcc ttaatgccga taccggtgtc attaacggaa aaaagaacct
     9721 ttactttatt cttttcctca tttttttctc ttagaacagc ggaaagcaca atcctccctt
     9781 tatcagtaaa tttagccgca ttgcttaaca gatttatcag aacctgcctt aatctgaact
     9841 gatccgtcct tacatattcc ggtatatcgg gagatagttt cacttccaga tctatcggtt
     9901 tttcaatgat ctgatgactt gtcaatccgg caacttcgtt aagcagggca gaaatattta
     9961 cagcatgata atcaagttcc agtttacctg cctctatctt tgaaaaatcg agaatgtcat
    10021 taacaacctc aagaagaatg tgtgccgatt ttgagacatt tgacatgtac tgtttctgaa
    10081 tatcactcag ctctgtatca atcagaagat ctgaaaaacc tactatcccg tttagcggtg
    10141 tcctgatctc atggctcata ttggcaagaa attcactttt ggcattgttg gcggactccg
    10201 cctccttttt tgccgatagt atcgtctctt caaccttttt tctttctgta atatctgtgt
    10261 gcgaacccgc catcctgtaa acgattccgt tctcatccct cagaccgtca cctctggcaa
    10321 gtatccagac atatgaaccg tttttatgcc tgaaccggaa ctctatttca tatttctttt
    10381 catgtccctt gaaataatcc tcaatgaaat gcatcaccct tatcttatca tcgggatgga
    10441 ttctcctttc aaatgaatca agtatattgg gaatttcatg atcttcataa cctatcattc
    10501 ccttccatcg cggagagaga taaagttcac cggtccttat gttccagtcc catattccgt
    10561 catgagaacc ggttactgca agcatgaacc tttcattact ttccgaaagt tcttttgttc
    10621 tgtttctcac aagaacttca agtgtcgatt ccctgttcct aaggcctgca atgaaaaaag
    10681 aaaagaggat gcaaagaaga aatatcagta taaaagttat cattcctatt atcaaactgg
    10741 tcgaaaacaa tggttgtggc ttctgtatca tatctattga aagagcactg ccgaatatga
    10801 acaaaggtcc tgatataact cttgacggat cattgcattc agaatcattg tctgcaatgg
    10861 aaacaagcgg ctctgctgtc ccgctttcag aaagcacctt cagatgaaga tgagagttgc
    10921 cgtctgagaa ctttaccaga ggataagtgc agttaatgag aaaatctgta tcgacagcgg
    10981 ttatcagtgc cccttttgcc ctgtctgtta aatttgaata aacaggagta atgacgaccc
    11041 attccatctc ccctttttca ttaaaattct gaatacttac agacattctg ctttttactg
    11101 caccagcaaa attctgataa aaataaggcg atgacaccca cttgttcttt tcaattacaa
    11161 acccgttatc ggacatcccg tgaacaggaa aagccctgcc gtcatcatta agctcaaccc
    11221 attgtaaata tgaaactata gcggacctca caacaggagc cgcaagtttt tcaaatatct
    11281 tatcaccgga ttctatagtc ccgatatatc ctgccattgc ttcaatattt tttctcacat
    11341 caaagaattc atgtcttaca agctgtgcct gtgcctgtgc aaggttcatt gaatcatctc
    11401 tttctgatat ctgcacaagt tttgatgcat tcattccgaa aacaagtgcc agcaggaaac
    11461 ccatgatgaa aatgagctca gcttcaaggt gtttgaaaaa ccatcttttc ttcttctttg
    11521 tcgaatccct tattcccatc attgagaaaa atgtaaaaaa tataagaacg accaacattg
    11581 tcctcaacat tgaagctgct ctggctatgt gaatctgttt ctgccagtca tcggcaagca
    11641 catcaattcc tataagtaga attatttctc cgctttttct tgaaaaaaca ggtgcgtacg
    11701 ctgaaacaaa ggttccatat tcatcggtga aagggccggc aactctcgga attccgtctg
    11761 caaaagattg tctcagcgga tcgtcgggca ttttgtaaat ttcacccggc atagatgcca
    11821 tcggatcgct ttcatcgtag tcctctggac cgaaaacaat gttcccctct ttttccgcca
    11881 tggaatatat ccctctcaaa tttccgagat attttccata agatatcatc tgtgaacgga
    11941 tcctctgaaa atgggagttg ctgatgtctt cctgagtaaa aagaagcttt tcgacataat
    12001 cgccgtttat cgttgaagct accgcctctg cctgaaaaag aatgtctttt ttaagattac
    12061 tgtcggcaag agatccttta tattcagcag acaaccagcc cagtaccaga attaccagaa
    12121 atgaaatata aaaatctttt ttaacacaga taatttttct ttcatatctc aaagagaaaa
    12181 aacggcttat aaaaaattta agcacttcta ctattataaa agcacccaat ataaagatca
    12241 gataatcaag ctggtttgca gtatagtaga tcaatcaaat cctcaaaaaa acttttatca
    12301 gaattttatc acattagaaa acaaaaaaca atttttggca caacttgttg ttttagatag
    12361 taagaactaa gcagccggtt gattcatctt cgggaacatt tgcattatcc ggcttatcat
    12421 ctgtggcaga actgtcagat ctttcctctt cgtcaaccct cttttcgaca tccggtgttt
    12481 ctgtattccc gttactgtca ggagattctg tttcaacatc attttcatgc tcttcaaccg
    12541 attcatcaac agtttcatcc ggagtttctt catcatcttc aaattcaacc ggttctccac
    12601 cgcactcacc acttgatgca agttcaatca tttttcctgc atcaacgatc cccgctccga
    12661 agcagacgct tttttctcca tgactgcatt cggcatccgg agctgaagcc gcttttttca
    12721 tgcaggatat cgcttccgga gctgtcattg aaggatttac agaaagcatc agcgcaagtg
    12781 cccctgcaac aacaggagcc gctgcacttg ttccgtcaaa atattttgtg tatgcctgat
    12841 ccatgtaatc ggtatagccc gaatttgcat cattgaaagc cggattaaag ccctttccgt
    12901 ttgcagtgac atctgttgtt atgagtcctg attttgcggg atctgcatca ccggcaggag
    12961 cagcgatatc aacatttatg ccgtaatttg aataatatac aagcgtgctg tcaacatcaa
    13021 gccctgagac aacaacattt ctgtcatcaa gcagggaagg attcctgact gtgtcacatg
    13081 cggaatttcc tgccgcccag acaatgatcg ttccaagacc gttgcgacct gtatttactg
    13141 cataatctgt catttcctta acaaaattat caagcggaat gtcaatgcac tgatcggtct
    13201 gactctctgt gtcgggagcg atagtccatg aattatttat gacccaggca ccggcatcaa
    13261 ctgcccattt tactgcatta agttttctgt ctgcaggagg attgctcatt tcaagtccga
    13321 tatacttgac tgggatgaca ctgcatttag gacacactcc gctcacacct attccgttgt
    13381 cgccctgtcc tgccgcaatt cctgaaacgg ctgttccgtg agccatgtaa tatttgttct
    13441 gcaacgcacc catattcggg tctatctgcg gttcacccgg gacaaagctc atgccgagct
    13501 gcagaaaaag atcgggatgc atggtatcca caccgctgtc aagaactgct attttagcgt
    13561 ccggataacc ctttgccttg tcccatgcag tttcagaatt tatcaccttg tgctgccact
    13621 ggtcaccgta ataagtgtca ttcggcacaa atgcaagtac aggtctttca agccagaccg
    13681 gttgagccaa tttaacaaaa cccgattcgt aaagctccct tgaaacattg aaaagctctg
    13741 tattttcagg tgttttaaag ataaaatatc cttcaattcc gtcaagcgtt cttgcaattt
    13801 taaaagaata tttttcaatg attccggcta tcactttttt actgacccct ttcctgaaaa
    13861 cgacagctat ttcacccgtc aaagggagct tcaaccctcc gtcaattgag accgacttta
    13921 cataactgtt tgattcgact gaaaaatcca tcctgatctt ttcaagacat gtgagttttt
    13981 ctccgtaata atagcacggc tcttcagcat aaatagcaga aaaaaggagc gtaaaggcaa
    14041 tgacaacaga taactttttc attttccacc tcaaaatttc aacacatcac cgcagtaatt
    14101 tgagcttttt aaatcacaat attcgaccgt tttcataaaa aaatgtcaaa agcatgttct
    14161 gagttctaaa tcggaacctt agaccttagt tgctgagaac ttgcctgact catagttaag
    14221 gagctcattg actatcagga taacttcgtg ctgaccaaac agtttctgct caccagtatt
    14281 aaggtttttc atcagtattt tcttgtcctt agcttcatct tcaccgataa tgaacatgaa
    14341 ttccgcccct gctttatcag ctttctggag cgcttttttg aacttcttcg gcttgtaatc
    14401 ggcaaaaaat ctgacaggat gatgtgacat gtgttttgca aaatggacaa ggtcatcaac
    14461 cgcttcatcg cagagtgcgt aacccgctga aataagaggt tcggtgtaaa aatcttcact
    14521 gatgagatca aaaagccgct caagtcccat tgcaaaaccg gtcgccgggg tgtcagggcc
    14581 tcctatatct gaaacaagat tgtcatatct gccgccgccg gcaacagcat tttgcgaacc
    14641 ccctgtattt gcaactattt caaaagcggt tcgggtgtag taatcaagtc ctctgacaat
    14701 gaaaggatca atgattccgt tgacatgaaa tatttcaaga gcatctttaa gctgtttgaa
    14761 atgggtatcg caactaccgc agagattatc ggtcataacg ggaatgtcat gatatttcgc
    14821 tttcggattg caggttttgc agtcaagaag tcgaagcgga tttgtatcta ttcttttctg
    14881 acagtcttcg cagaggtgtt ccttttttga agagaaatat tcaacaagtt tctctctgta
    14941 tgcaggtctg cactctttgc aaccgattga atttatatgc acttcgtagt caattttgaa
    15001 gtgttcaaga actctgcagg ccgtaaatat catctcggca tccatctgcg gagtttcgtc
    15061 cccgaagatc tcgattccaa gctgatggaa ctgacggtat ctccctttct gaggtctttc
    15121 atacctgaaa aaagggccga tatagtaaag tttgaagggc atcggttcgt tcattgctat
    15181 tccatgctca ataaaagccc tcaccacact cgcagtccct tcaggtctca tcgcgacatg
    15241 gcgtcccttt ttatcaagaa aatcatacat ttccttctgc acgacatcag tcgtttcacc
    15301 cactcccctt ttaaaaagag ctatctcttc aaggattggg gttctgaact ctgtgaaatt
    15361 cgcattttca acgatccttc tgatgtcctc ttccattctg ccccagcgca tcgcccttct
    15421 gccgaacaga tcgttcatcc ctttaactgc tgaaatcatt acaacacccc ttcaaatttt
    15481 gtttcgatat ctgaataaat ttcatccatt ttgagatttc cgttaagtct gacaacttta
    15541 acatgttccg aaaaatcgga tatcactgtt tcataatttg cctttatctg ctcaagatac
    15601 tcccttgtct catatagttc tgtgctatgt ctgaaaattg aaagccgctc aagtgcctta
    15661 tctatcgaaa catcaataaa aacaagtata tctggcattg tagcaaacct gttcacccct
    15721 ttcacccatt catagctcac attgacagac tgatatgcaa cggagctgaa aaaatatctg
    15781 tcggtgatga cagttctttt agctttaagg gcaggccaga tcctttcctt caagtggtta
    15841 agccggtctg cagcaaacat cagtgcaagc atttcgtgcg taagcggcga atcagagtct
    15901 tttctggaaa gcacttctct tattattttt ccggaagaca tatcggtcgg ttccgcagtc
    15961 agcagaacat cttttccttt gcttaaaaga tatctgctca attctttaat tgttgtgctc
    16021 tttccgcttc cgtcaatccc ttcaactgca atgaacatta ctattctcct gcaaatttat
    16081 atatcttcag atcatcaaaa aacacttttg agcgccagtt tccaaaagag aacctgtcaa
    16141 aaccgtcact tgttttaagc ggatcatcat cattatatga cataaaatgt ctgtcattga
    16201 caaaccactt gatatcactg ccttttctca caattctaac tctgtatctc tctccttttt
    16261 tcaaacggac atctctctct tcagaccttt tcttttcgtg ttcgtggagc ttggaaatga
    16321 cagaaaccct gttctgccag ccgcccagaa tgaaagaata tccatcaccg tgatcgtgaa
    16381 ttttcccatc accccatccg ttgaatttta catctacacc ttcactttcg ctccacattg
    16441 tcagctcgat cacaccgttt tggggaagag ccgctcccgt cagaaccaga tttctgttct
    16501 cagcttttga gcttgtgatc gctccttcct ctatcttcca gtcaccgccc tgaacagtat
    16561 aattttctcc aagttccgcc ctctcaaaat tgtcttcaaa tatcttctca agttttgctt
    16621 cagaacacga aataagcaga aaaacggctg aaaacagaaa aaaaactctt tctttcacct
    16681 tttacctcct taaaaacaga ggaaaatatc aaatatagcg atgaatgtca aaaatgtgga
    16741 aacaattccc aaactcggcg gacttgttca cagatatgag tggaaaaaaa cattttttaa
    16801 cactacaggt ttcaaatgat caattacttt actgattttg aggttttttt agattcactt
    16861 gacatattaa agacaattta atacaaatcg agcgtaactt ttctctaaga atttgtagtt
    16921 gttttttcac aataatttat gaggaaaaaa atgaaaaaga ttctttctgc tttaatgttt
    16981 tttggatttt gcatgttttt atactccgat tctttaaacg aaagctcaga tccggacgac
    17041 aactgggttt caataagtga acatcagggt gttggatgga atgggctaac atctgtaaaa
    17101 tcaattcatc actatgaaga ttatatttat gttggtggtg aatttagtgc agttaaaagc
    17161 actttggcaa atagcgttgc cagacttcat cttccgacgg gaatctggag tgctttgggc
    17221 tcgggattaa atggcggggt tgtttcattg tcaattgtaa acagtgaaaa aatatttgcc
    17281 gccgggcagt tccaattgga agacgacgat cattattatg acggtgttta tatgtggaac
    17341 ggagaaaact gggaagtgat ctctttagat tttgatggtg atatattcag tactgcaatt
    17401 gataaaaacg gcaatctcta tgttgcaggt gagtttcgta atatcggaga tgttgaggta
    17461 aacaatgttg caaagtggga tggaaaaaaa tggagttctc ttgggaacgg atccaactat
    17521 gaagtgacga atattatgtt cgataaagaa ggaaatcttt tggctaatgg aggtttttta
    17581 tcatctgatg aaaaatcagt ttattatttc gcaaaatggg atggacagaa ttggatttct
    17641 caagaaactg gtagtcttaa aaaagtgagt gcatttacga tcggcaagga tggttatttg
    17701 tatttgatag gattttttga tgaatcatat ataggaaaaa ttgttaaatg ggacggaaac
    17761 gactggactg ttttaaatga aaactcta
//
LOCUS       DAOOYB010000027        17662 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_3100_length_17662_cov_10.888453, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000027 DAOOYB010000000
VERSION     DAOOYB010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 17662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17662
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3100_length_17662_cov_10.888453"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2718)
                     /locus_tag="PL056_02765"
     CDS             complement(<1..2718)
                     /locus_tag="PL056_02765"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HPM45970.1"
                     /translation="MNTNFFNKSYNPEDFLIFIRNNMFPRIDFGLKRKSFSTGDFSLN
                     KIKSITQIGESEKLDIALYEITHESENDPRVTLTREAFRFMRDNVDTYTSNALIIFRS
                     LNSENYRFSLLTTDYKRVGKKTEKELSNPRRFSFYLGPDAKVKTPEEFLIKKGAVADI
                     EDLKSRFSVEVVNKEFYLKIADLFYRFIKEAKYPRDSRKKEFAVKLIGRIIFCWFLKK
                     KKSANGVPLIPEELLSLKSLEKFKDYFHSVLEPLFFEVLNTPVNKRKFENLLQIDELS
                     KTIPFLNGGLFEPDNDDIYSKGTGTFNIISIEDSWFSDLFKLLEMYNFTINENSPLDM
                     ELSIDPEMLGRVFENLLAELNPETGETARNATGSFYTPRPIVEYMVDESLKAYLSQNA
                     GIKEQNLDKLLSWSDENPELSKDQKSDILNALDKMKSIDPACGSGAYPMGILQKTALI
                     LEKVDPDSIEWVMKIVDRISDPIAKTIVEDQLQNEDWSYIHKLGIIQNSIYGIDIQPI
                     AVEISKLRFFLSLIVDAKIDDSAQNRGVKPLPNLEFKIVCADTLYKSPEPLNLETLFG
                     REPFFVELEKLVKSYFGANNDVRKDKLKEEIKSHIKKKVKEKKDAINYALKPAKGTKF
                     EQVWGKEKEAEVDKLNNELELWDSYTNIFNHQKVEFFDTRYFFPDVKDGFDIVIGNPP
                     YIQLQNNNGYLGEKYKDQEYISFAKTGDIYALFYERGADLLKDGGHLCYITSNKWMRA
                     KYGEKLRGYLSEKTIPKILIDFGGYKVFDSATVDTNILLFEKTTHPGKLKACKIEKDF
                     KSSMNIADYFNKNHIDLTGLKSDSWIISSADESIIKRKIEKIGTPLKDWDIQIYRGVL
                     TGFNEAFIIDGATKDKLIKEDPKSAEIIKPILRGRDIKKYKAEFADQWLI"
     gene            complement(2789..2944)
                     /locus_tag="PL056_02770"
     CDS             complement(2789..2944)
                     /locus_tag="PL056_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45971.1"
                     /translation="MVDFEKILLDKLPDVLDEKQKKNKVKNNLQVLRRKGEIYIDGKV
                     WRMSKLK"
     gene            complement(2944..6201)
                     /locus_tag="PL056_02775"
     CDS             complement(2944..6201)
                     /locus_tag="PL056_02775"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HPM45972.1"
                     /translation="MTGKLITNDTHILAEKMRNVIPNCENIYALVGYFYFSGFSELSE
                     DVKNKKMKILVGMDIDALISGKLREVSLIEKIENESSDIEIRKEYYEKLTTLINETDS
                     FDSSRQEKSLKLFIEKIKDGTLEIRKTREPNHAKIYIFENDRNHNENGDYLGIVVTGS
                     SNFSYSGLAGRNEINVMFRDNRDFEEAKTLFEKLWNNSVEIVGQYNKDDFFTEVKEKI
                     WTEKIPAPILLFIRALSEYFSVKQDKSIKLPDEINKKYANFKYQSDAIQKALTVIERH
                     NGVIVADVVGLGKSVIASAVAHNLKLPVIIIAPPHLTDQWENYRMQFNYNARVFSSGK
                     IEDALNKQCFDEKYLIIIDEAARYRNPETKDYALLHKLCQGNKVILLTATPFNNNPED
                     VFALIQLFQIPAKSTIRTVQNLSGAFRELIKEYSALQKLNRSGSIKETELKDEIRRIS
                     MKIREIIFPVVIRRTRIDLDIIEEYKNDLVKQGIHFPIVNEPVLERYDLGKLAPLYES
                     TTEEIYPKNEEKKSGKESFKGARYRPLSYLKPEALERMKNEKTQSEYRNLEYLIEGQK
                     HIAGFMRTLLVRRFESSIEAFRKSVDSILSASEEMQKWFEKGRIPVRKKGNVVEVDVL
                     LNSNGEELEMFSSEVNEIIEELKEKGYNFFLSSDLNDEFITDLKKDIHLLKKIKKEWE
                     NVKNDPKLEAFSNILKEQIRKEPERKIIVFSEFADTVSYLSDKLKYGLQTMKYTSADS
                     TRENKRRIEQNFDAGLDMKEQLNDFNILIATDAISEGYNLHRAGTIFNYDIPYNPTRV
                     IQRIGRINRINRKMFDSLKIYNYFPTDKGEKETGIKTISTFKMAMIHALIGEDAKVLT
                     NEEEVNRYFAEKLCEEKKAQEEESWDAKYRNQLYFVKKLTPELFEAALTLPFRSRVKR
                     TKRVEKSGVLVFGRKGEECSFKFSNSDGVIEEITIETAFKLMESDISEKGDKVSEAFD
                     ILYQNTKSHLFTHSTSSKGDKKQTETTVAIKSFINTNPEKREYLSELLYTVSTLKAIP
                     ENYMKMIRNIDSANEKEEIEALEKEIPIEYLTKIRKRAREIDGGRESIILAEELI"
     gene            complement(6211..6744)
                     /locus_tag="PL056_02780"
     CDS             complement(6211..6744)
                     /locus_tag="PL056_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45973.1"
                     /translation="MKKVLFLIAVCSTTIFPSCGKIDTGNKNTSFIQNIATPYCEKVE
                     ECDPETFNNSFDDVENCSEEFISDTTDWYKGKSTQCHKAYESLITCMVELDCDDFSHN
                     QTDSNPCYDKMVDYYNLCEATEEPDEESVCSEGEKKCEGDSFYWCIEGKWYSQNCYEN
                     NQKTCDENKGCVTEDLF"
     gene            complement(6764..7102)
                     /locus_tag="PL056_02785"
     CDS             complement(6764..7102)
                     /locus_tag="PL056_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HPM45974.1"
                     /translation="MSDCIFCRIIEGRIPSTKIYEDEFFIVIKDISPLYKVHNLIITK
                     KHIASVNEVDESDGAIFASIFSVARKVAEIENIADSGYRLTVNSGKDAGQLVPHFHMH
                     ILGGEKLSEL"
     gene            complement(7099..8499)
                     /locus_tag="PL056_02790"
     CDS             complement(7099..8499)
                     /locus_tag="PL056_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45975.1"
                     /translation="MRKINLTLTFFLYSILSYIFYIIVFVFVKNSSYESGIYYFTPAF
                     IFVFIKLFESGIKKIISVALFVTVPFLISVYLTLQGHLAGWEVSGFIPVIVLISYLFK
                     STDTGATPLRFVHLDRAVWMFFATLLLFASIHFIKTGSIDHALYLAVSIYSPAPAAVV
                     TALFINFIISTAAVSAILNNLRFFNNGAKITRLVFDTDRFLTFPHFNLSGIETVKDVS
                     TIEFLELVEKLNLAASESPEYSQNLKKDKLFSKTFEDGMSLAMAPLNIILSNNGYSHE
                     EIALPEKNDDRSFIALARNSKIIGYYAIDRIKPSTNCSYLELIEKRHGIKSVIIAPEE
                     PELWNSCCEIKRSFSETEITDSDLIITDEPEENTTGTQVVWGKCDSGKGDIYIAKPFI
                     TTIHNIIILSSGLKSRLVKGIIFCSFPFAFSLFSISFGLTLPKISTVSVLFSFVFTMI
                     YIFYMKPSNRDKGVKK"
     gene            complement(8518..9126)
                     /locus_tag="PL056_02795"
     CDS             complement(8518..9126)
                     /locus_tag="PL056_02795"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HPM45976.1"
                     /translation="MEKVSIHLQNPQTKTLETIIAVLNEGGVVLLPGDTSYFLAIKMG
                     KKNALEKLNRIKQTKKRKYYSVVFKDLGDISKYADIDNRHFNLLKRCLPGPYTFILTA
                     SRDIPKIMLENRKEVGIRIPESRFISELLELFGEPLVVSTASSDEGGDFNDPDEDEPQ
                     WLHMIDLLVDGGYVPSELTTIVKLNGDEYEIIREGKGSPDIF"
     gene            complement(9130..13440)
                     /locus_tag="PL056_02800"
     CDS             complement(9130..13440)
                     /locus_tag="PL056_02800"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45977.1"
                     /translation="MSKIVISDDRSLENLDIRFDHLLLGGDDIREESEKFIPLENMLQ
                     RIPQEFFPSLLMSSTKEKAVSVLSGDDELELLFLIILAGERSEELLEKLKSLKNSMDP
                     EKYRDFLIFLFLHSKNGKENIISEIRSSTLPGKDGILDLILAAYDAEPHEIKPLKGAE
                     YLYATFFEALKAEQAGDHARAFSLMLALFERSMYHPFIFEVLKFYIVQYDGISPEQIE
                     IFSSKVTESPLTVPFSDLKFIEFLYYFRNNIENKLEFAVSALAESTDSIFLLNVIAPL
                     LYKYKKWHLIGKFYKLSAKKTTGSERTKYLELLADIYENKLDMPDFATEIHKNIVEDD
                     PMSCSVSLSRVLSVYEENGMWSELFNLYNHLSGREEDETLKAYYFYKAGDVLHRELGK
                     SAEAREYLEKSLAIKHSFEVVRTLSEIYLKMHDYDAYIKTLLRELEFSLEASERIRVL
                     NIIAETYINHKKDFISAQKYLLSILDIAPDHLPTIKKLGKIYYQTRSWKNLAEINFKE
                     IDLTKDLVDTVNLYYRNGSIFFKELGDLERATECFMEILEIEPDHIPTLLYLEKIYLR
                     KRDINNLIILYKRLLEQSHTDSETRQYYLTRLGIIYRDNNMVNEAIDVFRKILKMFPE
                     NIMARENLRMLEGKPDFSESNTGDYIEKDLELFSDLIKNKDHSYVTSQYLLKKEPSLW
                     KYLYFIRKDSRSDIEKPQKPGHDEQFVISLLEQNYSIDNLIKNSSKKIALMLLVEKYI
                     EEGYFRGIQTILKYYLKYEPENKRKVWSIFFRGSDNPELKEELENILLNESDPFSIEV
                     VKDILEKIYLKEEDYNTLIFLRNFFIKKISDPKRKCDLIDSTIELVHEHAGTEQLLDL
                     YKLRYSSTPDEEICAYREIYEKFLSSIGKTEMLTNIYELEWNKNHDICAGKKCFEHYM
                     STGNIEKAVQMARAILAAEWDFSLFMKLVELLKSSGDINLAISDVTARIDNSPEDFRE
                     QLKGLLLDLNLEAGYTANALDLFNKTQYSDNSDRFERGMKLIKQMNTEKPDELTISLV
                     RSLVSSNTDQAVEKLKFLFQSGTDISETDIQPFSSYNEVLHAFDGTLPEEYKARILTH
                     FAQRNDVDAKKVLINNYIKEGDEENAVKLIDSIDPDPVSKTVFLSKLAWKNGQYQKEK
                     ELLKGILFTAIAKKDTYPLKRLAILEKDNRRLQFFIESILKLSGLSDKTDEELYPRIF
                     AIDSKSIFEFLNFTQTELLLKEFLHITSFGIRDETKIPGKPLNSNSHRELVNLIEYIK
                     LSANFDDLTGVFDDKLESPFEIVQAKLPCICFGPAALKMSIRDLKFKVIRNAFLLSCG
                     MRNTFNRDNAGFSSSALIAPLKLEGKEKVRFIKNIRPNFQNRLLDLVTKLENTEPDTF
                     TTFLEKLYDASFYHTFSLVPDIQTAFDFTGNDIEHIADPNPKFQQVFKFINNFFFQQV
                     "
     gene            complement(13456..14988)
                     /gene="guaA"
                     /locus_tag="PL056_02805"
     CDS             complement(13456..14988)
                     /gene="guaA"
                     /locus_tag="PL056_02805"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018294408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HPM45978.1"
                     /translation="MDKVLILDFGSQYNQLIARRIREMNVYCELHPFDVTIDFIKEFA
                     PVGIIFSGGPNSVNDAGSPDVSPEIFKLGIPILGICYGMQLMSVRLGGRLESGDSREY
                     GRAMVENLAPKSPLFRNVSDSTQVWMSHSDHVSVLPSGFYPIAKTNSIPYAAIGNDET
                     KMYAVQFHPEVIHTVEGGRILSNFLFEVCNASAEWKMENYIATAVEKLHDQIKDKKVV
                     LGLSGGVDSSVTAALLAKAIGKNLTAIFVDNGLLRKNEREEVEHNFKDMVNLIVVDAR
                     NEFMSKLAGVDEPEKKRKIIGNTFIEVFEREAAKIKDADFLAQGTIYPDVIESSSSKK
                     GASHTIKSHHNVGGLPEDMKLKLVEPLRDLFKDEVRELGLKLGLAEKLIKRHPFPGPG
                     LAVRILGEVSAEKCDILKDADHIFIEEIRKAGIYDSISQAFVVLLPVKTVGVMGDCRT
                     YEFVVSLRAVKTDDFMTADWFEFDHAFLKRVSSRIINEVKNINRVVYDISSKPPATIE
                     WE"
     gene            15169..15441
                     /locus_tag="PL056_02810"
     CDS             15169..15441
                     /locus_tag="PL056_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004102608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPM45979.1"
                     /translation="MEVKIEKLTDTQLKEKGVFSWGIWEKEVSRFPWHYDSQEECYLL
                     EGEVEVTDDTTGSVYKFGKGDFVTFPEGMNCRWNIRKAVRKHYNFK"
     gene            15581..16642
                     /locus_tag="PL056_02815"
     CDS             15581..16642
                     /locus_tag="PL056_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019217201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HPM45980.1"
                     /translation="MNEIEVKLGQDYKNWLTALKLRIRTVQLKAAVAVNTELLTFYWE
                     LGQDIVTKQKTSAWGDGFLKQLSADLTSEFPEMKGFSISNIKYIKQWHLFYGKPDEKS
                     QQPVGQITENIFSQITRIPWGHNIAIVSKCKTITEALYYVQQTIENSWSRSVLVHQIE
                     SGLFSREGKSITNFEQTLPKPQSDLAQQTLKDPYVFDFLTMTKSYNELDLEKALIDHI
                     AHFLLELGAGFAYLGRQFPLHVGERDFFIDLLFYHARLHCYVVIELKTVDFEPEHTGK
                     LNFYIKAIDEKLRKEGDAPTIGILLCKSKDKMVAEYALSDIHKPMGVSEYQLTQSLPE
                     NLKGSLPSIEEIETELSRE"
     gene            16737..16985
                     /locus_tag="PL056_02820"
     CDS             16737..16985
                     /locus_tag="PL056_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45981.1"
                     /translation="MQRSENICTYGDPTPLVASRLKMLEGYIAVFIKGKIVFIKFNLR
                     KEIVRFKPLIDMSFGNILPIPPLEVNLFSLGEAIKAFK"
     gene            16996..>17662
                     /locus_tag="PL056_02825"
     CDS             16996..>17662
                     /locus_tag="PL056_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45982.1"
                     /translation="MKKRLIILIVFLLFLLSCGGVEETDSDSDETEDIETSDTDTFDG
                     SEVIKFKDVNLEKCVRTKTQKPEGEITVEDVKDVEYLYCDEKGVVVIDGVEKIINLNY
                     LSLMRNDVKDFSLLKNLKSLKTLGINGNVNVDIKTLGELNSLEDLRADSCGLTELGYI
                     VKLAGLKKLVIQSNNIKSIEEIKNLTGLKNLSVPTNYLKNDDLPVLLNLVNLEHLNIR
                     ENCI"
BASE COUNT         5018 a         3329 c         3399 g         5916 t
ORIGIN      
        1 tatcagccac tgatctgcaa attcagcttt gtattttttg atgtctcttc ctctgagaat
       61 cggtttgatt atttctgctg atttaggatc ttcttttata agtttatctt ttgtcgctcc
      121 atcaataatg aatgcttcgt taaagccggt caatactcca cgatatatct gaatatccca
      181 atctttgagc ggagttccga ttttttcgat ttttcttttt atgattgatt cgtcggctga
      241 tgatattatc cagctatcag atttaagtcc tgtgagatca atatggtttt tattaaagta
      301 atcagctata ttcattgatg atttaaaatc tttttcaatt ttacatgctt tcagcttacc
      361 ggggtgagtt gttttctcaa aaaggagtat gtttgtatca accgtagcac tatcaaaaac
      421 tttatatccg ccaaaatcaa taagaatttt cggaatagtt ttttcagaaa gatatcctct
      481 cagtttttcc ccatattttg ctctcatcca cttgtttgaa gtgatataac acaagtgtcc
      541 gccgtcttta agaaggtctg ctccgcgttc ataaaaaaga gcatagatat cgccggtttt
      601 tgcaaaagaa atatattcct gatctttgta tttttcacct aagtatccat tattattctg
      661 aagttgaata taaggcggat tcccgataac aatgtcaaaa ccgtctttaa catccgggaa
      721 gaaatacctt gtgtcaaaaa attcaacttt ctgatgattg aatatgtttg tgtaactgtc
      781 ccagagttca agttcattat tcagcttatc aacttcagct tccttttcct tgccccagac
      841 ctgctcaaac tttgtccctt ttgcgggttt gagagcatag tttatagcat cctttttttc
      901 tttaactttc ttttttatat gactttttat ctcttcttta agcttatcct ttcttacatc
      961 attattcgca ccgaaatagc tcttcaccag cttttccaat tcaacaaaga aaggctctct
     1021 gccaaaaagt gtttcaaggt taagcggctc aggtgattta taaagagtat ctgcacaaac
     1081 gattttaaat tcaaggttcg gaagcggttt aacgcctctg ttttgagcag aatcatcaat
     1141 tttagcatca acgatgagag aaaggaaaaa ccggagcttt gatatttcaa cagcaattgg
     1201 ctgaatgtct atgccataaa tagaattttg aataattcca agcttgtgaa tatatgacca
     1261 gtcttcgttc tgcaactgat cttcgacaat cgttttcgca ataggatctg aaattctatc
     1321 aactatcttc attacccatt cgattgaatc agggtcaact ttttcaagga taagagctgt
     1381 tttttgaaga attcccatcg gatatgcacc gctgccgcaa gcagggtcaa tggatttcat
     1441 cttatcaagt gcattaagaa tatctgattt ctgatctttg gaaagttcgg gattttcatc
     1501 tgaccagctc aacaatttat caaggttttg ttctttaatt ccagcatttt gagaaagata
     1561 tgctttcaaa ctttcatcaa ccatatattc aacaataggt cttggagtgt aaaagctgcc
     1621 cgttgcgttt cttgcagttt caccggtttc aggattgagt tcagcaagaa gattttcaaa
     1681 aactcttcca agcatttcag gatcaataga aagttccata tcaagcggac tgttttcatt
     1741 aatagtgaag ttatacattt caagaagttt gaaaagatca ctaaaccagg aatcttcaat
     1801 tgatattata ttaaatgttc cggtgccttt tgaatagatg tcatcattat caggttcaaa
     1861 aagtccaccg ttaagaaagg ggatcgtttt tgagagttca tctatttgga gaaggttttc
     1921 aaactttctt ttatttaccg gtgtattaag tacttcaaaa aagagcggtt caagcacgga
     1981 gtggaaataa tccttgaatt tttcaagaga tttcaatgag agaagttctt cgggaataag
     2041 cggaacaccg ttcgccgatt ttttcttttt cagaaaccag cagaaaataa ttcgaccgat
     2101 aagttttacg gcaaattcct tttttctgct atctcttgga tattttgcct ctttaatgaa
     2161 acggtaaaac aaatctgcaa tttttaaata aaattccttg tttaccactt caactgaaaa
     2221 tctgctcttc aaatcttcaa tatctgcgac ggctcccttt tttataagaa attcttcagg
     2281 agttttaact tttgcatcag gaccaagata aaaggaaaat cttctcggat tggaaagctc
     2341 tttttctgtc ttttttccga cccttttgta atcggttgta agaagagaaa accgatagtt
     2401 ctcactgttt aaagacctga atattatcaa ggcattggaa gtgtaagtgt caacattgtc
     2461 tctcatgaat ctgaatgctt cccgggttag tgttactctc ggatcatttt cactttcatg
     2521 cgttatctca taaagtgcaa tgtctaattt ttctgactcc ccaatctgag ttattgactt
     2581 gattttatta agagaaaagt cgcctgtact gaaagatttt cgtttcagcc cgaaatctat
     2641 acggggaaac atattgtttc tgataaatat taaaaaatct tcggggttgt acgatttgtt
     2701 aaaaaagttt gtgttcatta tttccgtgct cctcaaaaat ctgtctaaaa atgtctaaat
     2761 ctgtctgaaa atgtctaaaa atgttttgct atttgagttt agacattctc caaacttttc
     2821 catctatgta aatttctcct tttcttctaa gtacttgcaa attgttcttc actttgttct
     2881 tcttttgttt ttcgtctaaa acatccggaa gtttgtctaa aagtattttt tcgaaatcaa
     2941 ccattatatc aactcctcgg caagaattat gctctccctg ccgccatcta tttctctggc
     3001 tctttttctg attttagtta aatactctat tggaatctct ttttcaagtg cttcaatttc
     3061 ttccttttca tttgcagaat ctatatttct tatcattttc atgtaatttt ccggaattgc
     3121 tttcaatgtt gacacagtat agagaagctc cgatagatat tctctttttt cagggttggt
     3181 atttatgaat gattttattg caactgtcgt ttcagtttgc tttttatctc cttttgaact
     3241 cgttgaatgg gtaaaaaggt gactttttgt gttctgatat aaaatgtcaa acgcttctga
     3301 aaccttgtcg cctttttctg aaatgtcaga ttccatcagc ttgaaagccg tttcaatcgt
     3361 tatttcttcg attactccgt cagaatttga aaatttgaaa gaacattctt caccttttct
     3421 tccaaaaacc agaacacctg atttttcaac ccgttttgtt cttttaactc tgcttctaaa
     3481 aggcagagtc aaggcggcct caaatagttc aggagttaat ttcttaacaa agtaaagttg
     3541 atttctgtat tttgcatccc acgattcctc ttcctgtgct tttttctctt cacacagctt
     3601 ttcagcaaaa tatctgttga cttcctcttc atttgtaaga acctttgcat cttcaccgat
     3661 cagtgcatga atcattgcca ttttaaatgt ggaaatagtt tttatccctg tttccttttc
     3721 tcctttgtct gttggaaaat aattgtaaat cttgagtgaa tcaaacatct tgcggttaat
     3781 tctgtttatc ctgccgattc tttgaattac tcttgtggga ttatagggaa tgtcataatt
     3841 aaaaattgtt cctgcccggt gcaggttgta accttctgat attgcatccg tagcaataag
     3901 aatattaaaa tcattcaact gctctttcat atcaagaccg gcatcaaaat tctgttcaat
     3961 tcttctttta ttttcccttg tggaatccgc agatgtatat ttcatcgtct gcaaaccgta
     4021 tttcagcttg tcagaaagat agctgactgt gtctgcaaat tctgaaaaaa caattatttt
     4081 cctttccggt tcttttctga tttgttcttt taaaatattt gaaaaagctt caagtttcgg
     4141 atcatttttt acattttccc actctttttt tatctttttc agcaagtgaa tatctttttt
     4201 aaggtctgtt atgaattcat cattaagatc actgcttaaa aagaaattat accctttttc
     4261 ctttaattct tctattattt cgtttacttc tgaagagaac atttcaagct cttcgccatt
     4321 agagtttaaa agaacatcaa cttcaacaac atttcctttt ttcctgacag ggatacgccc
     4381 tttttcaaac catttttgca tttcttctga agcagaaagt attgaatcaa ctgattttct
     4441 aaacgcttct attgagcttt caaatcttct gacaagaagt gttctcataa atcctgcaat
     4501 atgtttctgt ccctcgatta aatactcaag atttctgtat tctgactgag ttttttcatt
     4561 tttcattctt tcaagggctt caggttttaa ataggagagc gggcggtatc ttgcaccttt
     4621 gaaactttcc tttccgctct ttttttcttc gtttttcgga tatatttcct cagttgttga
     4681 ttcataaaga ggagcaagtt tcccaagatc gtaacgctca agaacaggtt cattaacaat
     4741 cggaaaatgt attccctgtt tcacgagatc attcttatat tcttcaataa tatcaaggtc
     4801 tattcgtgtt cttctaatta ctacaggaaa aattatctcc ctgattttca ttgaaattct
     4861 tctgatttca tcttttaatt ctgtttcttt tatactgccg cttctattca gcttctgcaa
     4921 agccgaatat tcttttataa gttctctgaa tgctccggaa agattctgaa cagttcttat
     4981 tgttgacttt gcaggaatct gaaagagttg aatcagagca aatacatctt caggattgtt
     5041 attgaacggg gtcgctgtaa gaagaattac cttgttaccc tgacacagct tgtgcagaag
     5101 tgcataatct tttgtttcag gatttctata tcttgccgcc tcgtcaataa taatgagata
     5161 tttttcatca aaacattgtt tattcaaagc atcttcaatt ttaccactgc tgaaaaccct
     5221 tgcattgtaa ttaaactgca tccggtagtt ttcccattga tcggtcagat gaggcggtgc
     5281 aattattatt acaggcagct ttaaattatg tgcaacagct gatgcgataa cagatttacc
     5341 aagtcctaca acatcagcaa caataactcc gttatgtctt tcaattactg tcagtgcttt
     5401 ttgaattgca tcagactgat atttgaagtt agcatatttt ttgtttattt catcaggtag
     5461 cttgatagat ttatcctgct ttacagagaa atattcagac aatgctctga taaaaagaag
     5521 tatgggtgct ggaatttttt ctgtccatat cttttcctta acttcagtga agaaatcgtc
     5581 cttgttgtat tggccaacta tttcaactga attgttccac agtttttcaa aaagagtttt
     5641 tgcttcctca aagtccctat tgtcccgaaa catcacattt atttcatttc ttccagcaag
     5701 accgctgtat gaaaaattgc ttgaacccgt aacaacaatt ccaagatagt caccattttc
     5761 attatgattt ctgtcatttt caaagatata aatttttgcg tgattcggtt ctcttgtttt
     5821 tcttatttca agagttccgt ctttaatttt ttctatgaag agttttaaac ttttttcctg
     5881 tctactgcta tcaaagctgt cagtttcatt aataagagtc gtgagttttt cataatattc
     5941 ttttctaatt tcaatgtcgc tgctctcatt ttcgattttt tctatcaaag acacttccct
     6001 cagcttgccg gaaataagag catcaatatc catccctaca agaatcttca ttttcttgtt
     6061 tttaacatct tcagaaagtt cggaaaatcc gctgaaataa aaataaccta caagagcata
     6121 aatattttca cagtttggaa taacattccg cattttttca gccaatatgt gagtatcgtt
     6181 tgttataagt tttcctgtca tttcatccct ctaaaataga tcctctgtga cgcatccttt
     6241 attttcatca catgttttct ggttgttttc gtaacagttt tgtgaatacc attttccttc
     6301 aatacaccag taaaacgagt ctccttcaca ctttttttcg ccttctgagc agactgattc
     6361 ttcatccggc tcttcagtcg cttcgcagag attatagtaa tcgaccattt tgtcatagca
     6421 ggggttgctg tcagtctgat tatgggaaaa atcatcacag tcaagttcaa ccatgcatgt
     6481 tatcagagat tcatatgctt tgtgacattg agttgatttt cctttgtacc agtcggttgt
     6541 atcggaaatg aattcttcgg agcagttttc aacatcatca aatgagttgt tgaatgtttc
     6601 aggatcacat tcttcaactt tttcacagta gggagttgct atgttctgaa tgaaggatgt
     6661 gtttttgttt ccggtatcaa tcttgccgca ggacgggaaa attgtcgtcg aacaaactgc
     6721 gatcagaaat aaaacttttt tcattagatg gctccttaaa ttatcacagt tcgcttagtt
     6781 tttctccgcc gagaatgtgc atgtgaaagt gggggacaag ctgtcctgcg tcttttcctg
     6841 aatttacggt gagacggtat cctgaatcag cgatgttctc aatttcagcc actttccttg
     6901 caactgaaaa gatggatgcg aaaatcgctc cgtcagactc gtcaacttcg ttaaccgaag
     6961 cgatgtgctt tttggttatt atcagattgt ggactttata aagcgggctt atgtccttga
     7021 taacaataaa gaattcgtct tcatagattt ttgttgaagg gatgcggcct tcgattatcc
     7081 tgcagaaaat acaatcactc attttttgac tcctttatct ctgtttgaag gtttcatata
     7141 aaaaatatat atcattgtaa aaacaaagga aaaaaggact gaaacagttg atattttggg
     7201 cagtgtcagc ccgaaagata ttgaaaaaag tgaaaatgcg aatggaaatg agcagaaaat
     7261 aatccctttg acaagacggc ttttcaagcc ggaagaaaga attatgatat tgtgtatggt
     7321 tgtaatgaaa ggttttgcga tatatatgtc accttttcct gaatcgcatt ttccccagac
     7381 aacttgtgtt cctgttgtat tttcttccgg ttcatcggta atgataaggt cgctgtctgt
     7441 gatctcagtt tcgctgaaag atctttttat ttcacagcaa ctgttccata gttccggctc
     7501 ttcgggagca atgattacag attttattcc gtgtcttttc tcaataagtt caaggtatga
     7561 gcagttagtt gacggcttta tcctgtctat tgcataatat ccgatgattt tactattcct
     7621 tgcaagagca ataaacgacc ggtcatcgtt tttttcagga agggctatct cttcatgact
     7681 gtagccattg ttgctcagaa tgatgttaag aggagccatt gcaagcgaca tcccgtcttc
     7741 aaaggttttt gaaaaaagtt tgtctttttt aagattctga gaatattcag ggctttcaga
     7801 agcagccaga ttaagttttt caacaagttc aaggaattct attgttgaaa catcttttac
     7861 cgtttctatt cctgaaaggt tgaaatgggg aaatgtgagg aatctgtctg tgtcaaaaac
     7921 aagtcgggtt atttttgccc cgttattgaa aaacctgaga ttgttgagaa tcgctgatac
     7981 agccgctgtg gagatgatga aattgatgaa aagcgctgtt acaactgccg caggggcagg
     8041 ggagtatatt gaaactgcaa ggtataaagc atgatcgatg ctaccggtct tgatgaaatg
     8101 aatggatgca aacaaaagca gagttgcaaa gaacatccag acagctctgt caagatgaac
     8161 aaacctgaga ggggttgcac cggtatcggt acttttaaaa agatatgaaa tcaggacaat
     8221 taccggaatg aaacctgaca cctcccatcc tgcaagatgt ccctgcaatg tcagatatac
     8281 ggagatcagg aaaggaacag tgacaaaaag cgccacagag atgattttct ttatgcctga
     8341 ctcaaaaagt ttaatgaaaa caaatatgaa ggcaggtgtg aaatagtaga tgccgctttc
     8401 ataactactg tttttcacaa agacgaacac aattatataa aaaatgtaac tgagtatcga
     8461 ataaaggaaa aaagtaagcg tcagatttat ttttctcata ttaagatcct gaatttatca
     8521 aaagatgtcc ggagaacctt taccttctct gattatttcg tattcatcac cgttcaattt
     8581 tactatcgtt gtaagttcag acggcacata gccgccgtca acaagaaggt ctatcatatg
     8641 aagccactgc ggctcgtctt cgtcgggatc gttgaaatca ccaccttcat cactggatgc
     8701 ggttgaaacg acaagcggtt ctccgaataa ttcaagaagt tcggaaatga atctgctttc
     8761 cggtattctt attcccacct cttttctgtt ctcaagcatt atcttgggga tgtctcggct
     8821 tgccgtcaga atgaaggtgt atggtccagg aaggcatctt ttcaggaggt tgaaatgcct
     8881 gttgtcaatg tcggcatatt ttgagatgtc accaagatct ttaaatacga ctgagtagta
     8941 ttttcttttt tttgtctgtt ttatccggtt gagcttttcc agagcatttt tctttcccat
     9001 cttaatagct aaaaaataag aggtatctcc cggaagcaga acaacaccgc cttcgttaag
     9061 aacagcaatg atcgtttcaa gtgttttcgt ttggggattt tgaagatgaa tagagacctt
     9121 ttccattgac tatacctgtt gaaaaaagaa attgttaata aatttaaata cttgttggaa
     9181 tttcgggttt ggatctgcaa tgtgttcaat atcatttccg gtgaaatcga aagccgtttg
     9241 aatgtcagga acaagtgaga atgtatgata gaacgatgca tcataaagtt tttcaaggaa
     9301 agttgtaaat gtatcaggtt cagtattttc cagcttggtt acaagatcaa gaagacggtt
     9361 ctggaagttc ggtctgatgt tctttatgaa cctgaccttt tctttccctt caagtttcaa
     9421 gggtgctatc aaagccgagg aagaaaatcc ggcattgtct ctgttaaaag tatttctcat
     9481 tccgcatgaa agaagaaatg cgttacggat cactttgaat ttgagatctc tgatgctcat
     9541 tttgagtgcc gcaggcccga aacagataca cgggagtttt gcctgaacta tctcgaaagg
     9601 actttcaagt ttatcatcaa aaacaccggt aaggtcgtca aagttcgcgg acagcttgat
     9661 gtattcaata aggttaacca gttccctgtg ggaattggaa ttcagaggtt ttccggggat
     9721 ctttgtttca tcccttatgc cgaaagaggt tatatggagg aattctttca gcagaagttc
     9781 agtctgagtg aaattcagaa attcaaaaat acttttgctg tcaattgcaa atatacgcgg
     9841 ataaagctct tcgtctgttt tgtcggaaag tcctgatagc ttaaggatgc tttcaatgaa
     9901 gaactgaaga cggcggttat ctttttcaag gatggcgagc cgttttaaag ggtatgtgtc
     9961 tttttttgca atggctgtaa aaagaattcc tttcagaagc tctttttcct tctgatactg
    10021 tccatttttc catgcaagtt ttgaaagaaa gactgttttt gaaacagggt cggggtctat
    10081 tgaatcgatg agtttgacag cattttcttc atcgccctct ttaatgtagt tgtttatcag
    10141 cacttttttt gcatcaacat catttctttg tgcaaaatgt gtcagtattc tggctttgta
    10201 ctcttccggc agggttccgt caaaagcatg aagcacttca ttgtatgagc tgaatggctg
    10261 gatgtctgtt tctgaaatat ctgtccctga ctggaaaagg aatttaagct tttccaccgc
    10321 ctgatcagta tttgatgaca caagtgatct taccagagaa atagtcaatt catccggttt
    10381 ttccgtattc atctgtttga tcagtttcat tcctctctca aaacggtcac tgttgtcgct
    10441 gtattgagtt ttattgaaca gatcaagtgc gttagcggtg taacccgctt caaggttaag
    10501 gtcaagcaga agccctttga gctgttcgcg gaaatcttcg ggggaattgt cgatccgtgc
    10561 cgtcacatca cttattgcaa ggtttatgtc accactgctc ttgagaagtt cgacaagttt
    10621 catgaaaagt gaaaaatccc attcggctgc aaggatagct ctcgccattt gaacagcttt
    10681 ttcgatattt cctgttgaca tataatgttc aaaacatttt tttcccgcac agatatcgtg
    10741 atttttgttc cattcgagtt cataaatatt cgtgagcatt tcggtcttcc cgatggaact
    10801 gagaaatttt tcatatattt ccctgtatgc acagatctct tcatcgggtg tggagctgta
    10861 tctgagttta tacagatcaa gaagctgctc tgttccggca tgttcgtgaa cgagttctat
    10921 tgttgagtct atcagatcac attttctttt tggatcggag atctttttta tgaagaaatt
    10981 tctaaggaat atcaatgtgt tgtagtcttc ttcttttaaa tatatttttt ccagtatatc
    11041 tttaacaacc tctatgctga aagggtcgga ttcgtttaaa agtatgtttt caagctcttc
    11101 tttcagttca ggattgtcag atcctctgaa gaatatcgac cagacctttc ttttgttttc
    11161 aggttcgtat ttgaggtagt atttaagtat tgtctgtatt cctctgaaat aaccctcctc
    11221 aatatatttt tcaacaagaa gcatcaacgc aattttcttg gaagagtttt taatcaggtt
    11281 gtcgatcgaa tagttctgct caagaaggga aatgacaaac tgctcatcat gtcccggctt
    11341 ctgcggtttt tcaatatcac tgcgcgaatc ctttcttatg aaatagagat acttccaaag
    11401 agaaggttct ttcttgagga gatactgact ggtaacatat gagtgatctt tgttctttat
    11461 aagatcggaa aagagttcaa ggtccttttc aatgtaatct ccggtatttg attcactgaa
    11521 atcgggtttc ccttcaagca ttctcaggtt ttcacgagcc atgatatttt cagggaacat
    11581 tttgagaatt tttctgaaca catcaatggc ttcgttgacc atgttgttgt cgcggtatat
    11641 tattccaagc cgtgtgagat aatactgcct tgtttcagag tcggtgtgac tctgttcaag
    11701 aagccgtttg tataaaatga tgagattgtt aatatccctc ttgcgcagat agatcttttc
    11761 aaggtagagc agagtgggga tgtggtcggg ttctatctca agtatctcca tgaaacactc
    11821 ggtagccctt tcaagatcac caagctcctt gaagaatatt gaaccgttgc ggtaataaag
    11881 gttcactgtg tcaacaaggt cttttgtgag gtcgatctct ttgaaattta tctcggcaag
    11941 gtttttccag ctccttgtct gatagtagat ctttccgagc ttttttattg tgggaagatg
    12001 gtccggtgca atatcaagaa tgctcaggag atatttctgc gctgaaatga agtccttttt
    12061 atggtttatg tatgtttcgg caatgatatt caacaccctt attctttcgc ttgcctcaag
    12121 cgaaaattca agttctctca gaagtgtctt gatataggcg tcatagtcat gcattttaag
    12181 gtatatttcg gaaagagttc tcacaacttc aaaagagtgc tttatggcaa gacttttttc
    12241 aagatattcg cgtgcttcag cgctttttcc aagttcacgg tgaaggacat cgcccgcttt
    12301 gtagaagtag tatgccttca gagtttcatc ttcctctctg ccggaaaggt gattatagag
    12361 attgaaaagt tcactccaca tgccgttctc ttcatatacc gaaagaactc ttgacagact
    12421 taccgaacag ctcatgggat catcttcaac gatatttttg tgtatctctg tggcaaaatc
    12481 gggcatatca agcttgtttt cgtaaatatc ggcaagaagt tcaagatatt ttgtcctttc
    12541 agaaccggtg gttttttttg cggagagttt gtagaacttt ccaataagat gccatttttt
    12601 atatttatac agaagcggag cgatgacatt gagaagaaat atggaatcgg tgctttccgc
    12661 cagtgctgaa acagcgaatt caagcttgtt ctcaatattg tttctgaaat aataaaggaa
    12721 ttcgatgaat ttcaggtctg aaaacggaac ggtaagagga ctttctgtca ccttgcttga
    12781 aaaaatttcg atctgttccg gagatatccc gtcatactgt acgatgtaaa atttcagcac
    12841 ttcaaatatg aaagggtgat acatcgatct ttcaaaaagt gccagcatca gcgagaaagc
    12901 tcttgcatgg tcgcctgcct gttcagcttt gagagcttca aaaaatgtcg catagagata
    12961 ttccgcccct ttaagaggtt ttatttcatg cggttcagcg tcatatgcgg caaggatcag
    13021 atcaagtatt ccgtcttttc cgggaagagt tgacgatctt atttcagaaa taatgttttc
    13081 ttttccgttt ttgctgtgta gaaaaagaaa tatgagaaaa tcgcgatatt tttcagggtc
    13141 catggaattt tttaaagatt tcaacttttc aaggagctct tcgcttcgct cgcctgcaag
    13201 gatgatgaga aaaagaagtt ccagctcgtc gtctcctgac agaacggaaa cagctttttc
    13261 ctttgtggat gacatgagaa gggatggaaa gaattcctga ggtattctct ggagcatgtt
    13321 ttcaagcgga atgaattttt cagactcctc cctgatatcg tctcctccga gaagcagatg
    13381 atcgaacctg atgtcaaggt tctcaagcga cctgtcatcg gaaataacga ttttactcat
    13441 tgtaacctcc tgttattatt cccattctat cgttgccggg ggctttgaac ttatgtcata
    13501 gaccactctg ttgatgtttt tcacttcgtt tattattctt gatgaaactc tttttaagaa
    13561 agcgtgatcg aactcaaacc agtcggctgt catgaaatcg tcagtcttaa ctgcccggag
    13621 cgaaacaaca aattcatatg tcctgcaatc gcccatcacc cctacagttt tgacgggcag
    13681 aaggacgaca aatgcctgag atatcgagtc gtaaatccct gcttttcgta tttcttcgat
    13741 gaaaatgtgg tcggcatctt tgagaatatc acatttttca gcactcactt cacctaggat
    13801 cctaacagcg agtcccggtc cagggaaagg gtgtcttttg ataagttttt cagcaagtcc
    13861 gagcttaagt ccaagctcac gcacttcatc tttaaacagg tcacgaagcg gttcaacaag
    13921 cttcagcttc atgtcttcag gcagtccgcc gacattgtga tgacttttga tggtgtggga
    13981 agcccctttt ttgcttgatg aactttcaat gacatcagga tagatcgttc cctgcgcaag
    14041 gaaatcggca tctttgatct ttgccgcttc tctttcaaaa acttcgatga aagtgtttcc
    14101 gatgatcttt cttttctttt ccggctcatc aactcctgca agttttgaca tgaattcgtt
    14161 ccttgcgtca acgacgatca ggttgaccat atccttgaaa ttatgttcaa cttcctcccg
    14221 ttcattttta cgcagaagtc cgttgtcaac aaatattgca gtgaggttct ttcctatcgc
    14281 tttggcaaga agtgcggcgg ttactgatga atctactccg cctgaaagtc cgagaacgac
    14341 tttcttgtcc tttatctgat cgtgaagttt ttcaacagcg gttgctatgt aattttccat
    14401 tttccattct gctgatgcat tgcacacttc aaacagaaag tttgaaagta tccgtccccc
    14461 ttcaacagta tgaataactt cgggatggaa ctgaactgca tacattttcg tttcgtcatt
    14521 tccaatcgcg gcataaggga tcgaattcgt ttttgcgatc ggataaaagc cggaaggaag
    14581 aactgaaaca tggtcgctgt ggctcatcca gacctgagtc gaatcggaga catttctaaa
    14641 aagtggggac ttaggtgcaa ggttttcaac cattgcccgt ccgtattctc tgctgtcacc
    14701 gctttcaagc cggccgccaa gacggactga catcaactgc attccgtagc aaattccaag
    14761 gatcggtatg ccgagtttga agatctcagg tgaaacatcg ggtgatcctg catcgttcac
    14821 cgagttgggg ccgcctgaga aaattattcc gacaggtgcg aattccttaa taaaatcaat
    14881 agtcacatca aaaggatgaa gttcgcagta aacattcatc tctctaattc tgcgtgcaat
    14941 aagctgattg tactgagagc cgaaatcgag aatcaaaacc ttgtccataa cacctccgga
    15001 atgaaaaaaa atccgcataa acaaaacagt aacgccttaa ataccataaa attaagaaga
    15061 actcaaagaa ttttatatca cttgaagggt gttcaaactg tttttatttc cattgtcgga
    15121 tcaaaagtgt aaaatcagtc gtcaatgttt aatttgaagg agttaaaaat ggaagttaag
    15181 attgaaaaac tgacagatac gcagttgaag gaaaagggtg tgttttcgtg gggtatctgg
    15241 gaaaaagaag tgagcagatt cccgtggcat tatgattcgc aagaggaatg ttatctgctt
    15301 gaaggtgaag tcgaagtcac agacgacaca acaggcagtg tctataaatt cggaaaaggt
    15361 gatttcgtca catttcctga agggatgaat tgccgctgga acatcagaaa agcagtgagg
    15421 aaacattata attttaagtg aattcacatg cttgcgcgtg aaaacatatg tacttatggc
    15481 acccctatgg ctcctatggc tggacaccta tggctgattg gggaatgtaa atgaaagcgg
    15541 tgggacttga aggatcacaa tagattatgg agttagggtt atgaatgaaa tagaagttaa
    15601 actcggtcag gattataaaa actggctgac agcactgaaa ttgcgtatcc gtactgttca
    15661 gcttaaagcc gctgttgctg tcaacacgga acttcttaca ttttattggg aattgggaca
    15721 agatattgta accaagcaga aaacgagtgc atggggcgat ggttttctta agcagttaag
    15781 tgctgactta acatcggaat ttccggaaat gaagggtttt tctatcagta atatcaaata
    15841 cataaagcag tggcatcttt tttatggtaa gccggatgaa aaaagccaac agcctgttgg
    15901 ccaaattacg gagaatattt tttcgcaaat taccaggatt ccatggggtc acaacatcgc
    15961 tattgtatca aaatgcaaaa ccattactga agctttgtat tatgttcaac agacaattga
    16021 gaatagctgg agtcgcagcg ttcttgtgca tcaaattgaa agcggacttt tcagccgtga
    16081 aggcaagtcc ataacaaatt ttgaacagac acttccaaaa ccgcaatctg atcttgcgca
    16141 acaaaccctt aaagatccct atgttttcga ctttctgaca atgaccaaat catacaatga
    16201 acttgatctt gaaaaagcgt tgattgatca tattgcacat ttcctgcttg agctgggagc
    16261 agggtttgcg tatctcggta gacagtttcc gctgcatgta ggtgaaagag atttttttat
    16321 agacctgctt ttttatcatg cccggctaca ttgctatgtg gttatcgaac tgaaaactgt
    16381 tgatttcgag ccggaacata ccggtaaact gaatttttac ataaaagcca tagacgagaa
    16441 actccgtaaa gaaggtgatg caccaactat cggcattctg ctttgcaaaa gcaaagataa
    16501 aatggtcgca gaatatgcac tcagcgatat tcacaaaccc atgggagtat cggagtacca
    16561 gttaacacaa tccctgccgg aaaaccttaa aggcagtctt ccgagtattg aggaaattga
    16621 aacagagctg agcagggagt aataaaatat aattcacttg aaaaaagtta ttcgaggaat
    16681 ggaaatgaga acatcaaaaa aacttgcgaa acttgttgaa aagacggaga aaatccttgc
    16741 aacggagtga aaacatatgt acttatggcg acccgacccc tcttgtcgct tcgagactga
    16801 agatgctcga gggatatata gcggtgttta taaaagggaa gatagtattt ataaaattca
    16861 atctcaggaa ggagatagtg agatttaagc cattaataga catgagtttt ggaaatattc
    16921 ttccgatacc gccgcttgaa gtgaatcttt tcagtctggg agaggcgata aaggcattta
    16981 aatagggaga tgcgcatgaa aaaaagactg atcatactga ttgtgtttct gctgtttctt
    17041 ctatcctgcg gaggagtaga agagacagat agtgacagtg atgagacaga agacattgaa
    17101 accagtgaca cagacacttt tgacggatcg gaagtgataa agttcaaaga tgttaatctt
    17161 gaaaagtgtg tgaggacaaa aacacagaaa ccggaaggag aaataacagt tgaagatgtt
    17221 aaggatgttg agtatcttta ttgtgatgaa aaaggagttg ttgtaattga cggggtagaa
    17281 aaaataataa atcttaatta cctgtcatta atgcgtaatg atgtgaaaga tttctcatta
    17341 ttgaaaaatc ttaaatcact taaaacgctt ggcataaatg ggaatgtaaa tgttgatatt
    17401 aaaactttag gtgaattgaa tagccttgaa gacttgagag cggatagctg tggtttaaca
    17461 gaactcggtt atatagttaa gcttgccggg ttgaagaagc tagttattca gtcaaacaac
    17521 attaaatcca tagaagaaat aaaaaactta actggtttaa aaaatctttc tgttccaacg
    17581 aactacctta aaaacgacga tcttcctgta ttgctgaatc ttgtgaatct tgaacatttg
    17641 aatatccggg aaaactgtat aa
//
LOCUS       DAOOYB010000028        17602 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_3127_length_17602_cov_8.948766, whole genome shotgun sequence.
ACCESSION   DAOOYB010000028 DAOOYB010000000
VERSION     DAOOYB010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 17602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17602
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3127_length_17602_cov_8.948766"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1535)
                     /locus_tag="PL056_02830"
     CDS             complement(<1..1535)
                     /locus_tag="PL056_02830"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HPM45983.1"
                     /translation="MPAMMSSQNKWFVFLFLMLFSFTIFADENGEEEGKPEKTVFKAG
                     EIVVTGNKSKAVIEDSATTTVIRAEDIEAKGASTLDQALEGVPGIQVYTHTKGHKRIR
                     MRGFDQEKIFILIDGVPINDIYSTDVDLSAIPVNTVSKIVVNRGVSSALYGTDGAIGS
                     INIITKKPDKLFVDSKAEYGSWNNSQFALSHGMPAGDFYYWFSGSVLLSNGFKPSQKL
                     DSTLRKKWYNKLLRYDRYGVTFDEILNPSKNQYIEDSGKWNHTEFTKYNAAAKAGWNP
                     VKWFEAGLSGDYHYSELKSNTYQNNCYSDYDPAQEVWKNARRPGFHDDPRYVQDWILR
                     NRSFVYPENWNYKISPYATINTEKFDMTFTGWFYQVYQSQKGYADNDHLYSKDVAAFN
                     GSKSTAITDPFLDIKKQMGYGFRILPAYKFSDNYKMSMMIHFRDEFFTAHEQAISMDE
                     SPGIIEKMGEDPYPVRDLRAQYFSIAVEDELVVKRFRSTAGISYDSQNFDEFKQRTEM
                     EYG"
     gene            complement(1516..2190)
                     /locus_tag="PL056_02835"
     CDS             complement(1516..2190)
                     /locus_tag="PL056_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45984.1"
                     /translation="MRSVSLFILGFFLIFSTISCSSDEAESDNDSGLADNETVTDDMS
                     DEVLTESDETQDEAVDDVIIPDNEENDEASDETADDEFSDDLSDEVLTESEENDEEND
                     EEVTDDALPPPSEEALLCCQEIVKEWFRCEGTYEVTAIQGCVDCFAALSVCADFYIAE
                     NSFGCSAECDAICEGTGSADNSGKDRRKVADGYCLFIMDKNCDIAPEDVSSFGVVDGL
                     DACYDE"
     regulatory      complement(2180..2302)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            complement(2342..2623)
                     /locus_tag="PL056_02840"
     CDS             complement(2342..2623)
                     /locus_tag="PL056_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016492083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HPM45985.1"
                     /translation="MFKVNQYFDGKVTSLGFNPGNGNATVGVMAPGEYEFGTSTVEYM
                     TVVAGALTVKLPGSDEWKEYIAGTTFKVEKDSKFQLKVKVDTAYLCEYK"
     gene            complement(2625..2831)
                     /gene="thiS"
                     /locus_tag="PL056_02845"
     CDS             complement(2625..2831)
                     /gene="thiS"
                     /locus_tag="PL056_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HPM45986.1"
                     /translation="MERTITVNGNVIEWSEGMTVADVLNVMKYTFKLLVIHVNDEIIK
                     REEWPSTLVPQGAEVKVIHLMSGG"
     gene            complement(2837..>4900)
                     /locus_tag="PL056_02850"
     CDS             complement(2837..>4900)
                     /locus_tag="PL056_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HPM45987.1"
                     /translation="YVNLTDNTISEKPVTQHMKEKFVGGKGFGLKLLWDGTKPETKWN
                     DPENEIIISPGPCAGITQYSGAGKSLVVSISPQTDSIMDSNVGGYFGPYMKFNGFDAI
                     ELQGKAQKDVIIIIDGRTNKVTIEEAPAEAMDSHILAEQLTEMYALDEKDKINIAVVS
                     AGSAADHSLIGMLNFSFYDPRRKLCRLKQAGRGGIGSVFRDKKIKALVAIGTGLKGDL
                     NNVADMKAIQERGQRFNKEMKLDDQQCEMRTKGTAHLMEIMNQYDLLPTKNFQYGRDD
                     SQIANIHSEVFKSKFTQTWKNLEGCWFGCSMSCAKAVDGFELKTGPYKGQKVIVDGPE
                     YETAAGLGSNCGIFDADHIIEANFYCDTYGVCTITVGTATAFVMECYENGILNKERTG
                     GLELNFGNSSSALELLHQMARGEGFGLIAGKGVRYMKNYFAEKGWGDIKFMNDIGMEN
                     KGLEYSQYVSKESLAQQGGYAMTNKGPQHDEAWLIFMDMVNNQIPTFENKAEALHYFP
                     MFRTWFGLQGFCKLPWNDIEPENNRQTAEPAKVPEHVESYVIIYNAVTGNNIDKHELI
                     RQSERVYNFQRVFNIRRGYGLRKHDAQPYRAAGPVTKAEYLSREAFYDKQLKELVNFD
                     PAGKSLEERMSVMRKYREDRYEQLLDAVYERRGWTKNGVPKIEHLKNIGMDLPEVIEV
                     VRPLQ"
     assembly_gap    4901..5000
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5001..5402
                     /locus_tag="PL056_02855"
     CDS             <5001..5402
                     /locus_tag="PL056_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014826871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HPM45988.1"
                     /translation="EAYEFYRDAAAKTKNASLKSIFNELAREEMGHKDLLETYAFDEL
                     RTIKFKEVKDYKITESVELPKLSTEMSFVDGIVLAMKKEEEAMNMYRNFAEVCADDAQ
                     KNVFLELAKMEQGHKAKLENIYNDTAFGESW"
     gene            complement(5405..8089)
                     /gene="fdhF"
                     /locus_tag="PL056_02860"
     CDS             complement(5405..8089)
                     /gene="fdhF"
                     /locus_tag="PL056_02860"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(7031..7033),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HPM45989.1"
                     /translation="MQIFINGIAVEAKPGQTIHQAARDAGIYIPTLCYHDKVGAAGKC
                     RVCVVEIEGMNGLQTSCSVQVREGMKIQTKTEKVVNSQKLIVDLLLSTGTHDCISCDQ
                     NGSCELQSVAYYLGIERGSFNYGMVEKNIDESSEFVYVDRNKCISCGRCVAACNGTVV
                     NEVLDFKYRGSKADIAFDADLPMGTSSCVQCGECSQICPVGAITDKKAKGLARYWEIE
                     KVYSVCPYCGVGCKLELHVDRKANKIIRVKGVEGASTNDGMLCVKGRYGYDFVNHPDR
                     LTTPLIKNPDGNFREASWDEAIDVVARKLFEIKEKHGADKIMGLCSAKVTNEENYTFQ
                     KLFRQVIGTNNIDHCARLUHSSTVAGLAATFGSGAMTNDIAGMDKSDVALIIGSDTSE
                     AHPVISARLKKAARKGKLKIIVIDPKRIRMADYAEIYACQRPGTDVAVMNGMMHLIIK
                     NGWEDKKFIEERVEGYEALKEEVAKFTPEYVEKISGVPAATLEKIAELFGKAETAALY
                     YAMGITQHTTGVDNVKTTANLQMLCGNMGKVGGGVNPLRGQSNVQGACDMGGLPNVFP
                     AYQKVGDDAVQEKFEKFWGVKLDNKIGMPMTVAFDKAYEGQMKAFYIMGENPMISDPD
                     LHHAEEALKKLEFLVVEDIFMTETAKLAHVILPASTYAEKSGTFSNTERRVQLLFEAV
                     KAPGTAKHDWEIHQMLANKMGQNWNYKCAEDIFEEIRKCTPSYAGLTYKRLGFNGMHW
                     PCPNEEHPGTPVLHIGKFTRGLGLMTAIPFKAPAEVPDEEYPMYLTTGRILQHFHTGT
                     MSRKSEGLNNLAGPHAMISVEDAEAIGVKNSEMVKVITRRGEITTKAFITKRCPKGTV
                     YVPFHFWEAPANKLTNNALDPVAKIPEYKVCACKIEKA"
     gene            complement(8102..>9145)
                     /gene="nuoF"
                     /locus_tag="PL056_02865"
     CDS             complement(8102..>9145)
                     /gene="nuoF"
                     /locus_tag="PL056_02865"
                     /inference="COORDINATES: protein motif:HMM:NF010120.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HPM45990.1"
                     /translation="KGRGGAGFPTGLKWGFLPKDTGKPVYLICNADEGEPGTFKDRQI
                     MEYDPHLLIEGMAISMLALGSKLSFIYIRGEFDWIADILDRAIEEAKKNGQLKDLDII
                     VHRGAGAYVCGEETALIESIEGKRGQPRIKPPFPAVQGLYGCPTIVNNVETLASVPYI
                     IENGADAFRKVDFKIFGISGNVKKPGCYEYPIGTSFKDLIEAAGGVDGKLKAAIVGGL
                     SVAILKAEELEDLKMDYTSCAAKGTALGSGGIMVINDEWSIPELALRTMKFYAHESCG
                     KCTPCKEGSKMTVRILEKIVKGQGTKEDLANVERLVGTIKGLTLCPVGEAFSTPIRAM
                     VEKFRPEFEALIK"
     assembly_gap    9146..9245
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9246..9535)
                     /locus_tag="PL056_02870"
     CDS             complement(<9246..9535)
                     /locus_tag="PL056_02870"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HPM45991.1"
                     /translation="MISENSLNKIKDLASRYPKKESAILPALMIVQKENGNALSKKDI
                     EGVAEILGVTHSKAFGVATFYTMYNVREKIGKYHIQVDTNIPATLMGAGKILA"
     gene            complement(9630..10898)
                     /gene="hydF"
                     /locus_tag="PL056_02875"
     CDS             complement(9630..10898)
                     /gene="hydF"
                     /locus_tag="PL056_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HPM45992.1"
                     /translation="MNSRTIRGDRLHIAFFGRRNAGKSSLINALTNQQVSIVSETPGT
                     TTDPVFKSMELMPIGPVVLIDTAGMDDLEDVLGEERVKRSEKVLSKTDLAIIVVDSAG
                     TAGDMEDDLIRQCGENKSSVIVVLNKIDKGLSESVRNWIVEKSFIRVSAKENTGIAEL
                     KSRIIEVAPSEWEPPFLKDLIHPGETVVLVTPIDLGAPKGRLIMPQVKAIRDILDGDG
                     VAVMCKERELLTTLNSLKNPPALVVTDSQVFSQVSADVPESVPLTSFSILSIRQKGDL
                     AEMVKAVLAVENLKPGDKVLIAESCSHHPLEDDIGRVKIPRWLNAHVGGGLKIETVPG
                     CEYPDNLDEYKLVVHCGACTLNRKEMLRRQSIPAKAQIPMTNYGVLISYLHGVFPRAL
                     KPFPEIYRLFEKNGLIQRKKKVKSINSDIL"
     gene            complement(10940..11248)
                     /locus_tag="PL056_02880"
     CDS             complement(10940..11248)
                     /locus_tag="PL056_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HPM45993.1"
                     /translation="MRFGLKEDVISRICSVFAEHPEVEKAVLYGSRAKGNFRNGSDID
                     LTLVGKDNLTLQKLYKIMEDIDDLFLPYSFDLSIFHQISDPEVVDHINRIGIVFYERK
                     "
     gene            complement(11250..11669)
                     /locus_tag="PL056_02885"
     CDS             complement(11250..11669)
                     /locus_tag="PL056_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase substrate binding
                     protein"
                     /protein_id="HPM45994.1"
                     /translation="MSNKDIRWIQRFKHFEQALFQLKKAVELANSRKLTELEEQGMIQ
                     SFEYTHELGWNTIKDFLEDQGHHSLYGSKDTTREAFKRGLIANGEVWMDMIKSRNLSS
                     HTYNREIAEKIAQAIVKDYLTEFEELSKVLRNIAEKG"
     gene            complement(11751..13139)
                     /locus_tag="PL056_02890"
     CDS             complement(11751..13139)
                     /locus_tag="PL056_02890"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF008909.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HPM45995.1"
                     /translation="MRKEKDFMGVVELDDSFPFGINTMRAMQNFNFNDEVMHPSIFKA
                     LLEVKKACAVSNRIAGILDEDTASAIEDGCDRAIAESIYPHLNPYQGGAGTSMNMAAN
                     ELIANYALKIIGKNYGEYEFINPLDHVNLSQSTNDVFPTAVKIATIRGIRQLHENIEK
                     LLHELQEKEHQFSGILKIGRTEMQDAMPVSLGQEFGAWAEGVSRFRWRLSKALDWIRE
                     VNIGGTAVGTGINADADYLRVIASELRKVVQEPLSVSRNGVDGTQNCDSIVEIMGIMK
                     TGAVVIKKIASDIRILSSGPECGIAELSLPAFQQGSSIMPAKVNPVMAEAAEQVCLEI
                     MASDNSVAHAVSQGNLELQQFMPFIAHKTVHSISLFSGMLKSFTKMISGITANETKIR
                     SYVESSTVVATLLAPTIGHERTAELVVEAREKGAKIYDLIIERKILSKEKLDMLLTPE
                     VMASPGMPVVEE"
     gene            13324..13488
                     /locus_tag="PL056_02895"
     CDS             13324..13488
                     /locus_tag="PL056_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004573472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPM45996.1"
                     /translation="MHKINASLCTNCGLCASECPVEAISENGGVHVISDACVDCGACV
                     SVCPVEAISG"
     gene            complement(13548..14474)
                     /locus_tag="PL056_02900"
     CDS             complement(13548..14474)
                     /locus_tag="PL056_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM45997.1"
                     /translation="MKKLSTLIAFLLLLAVIYPFYHVRKHLMENDKPLVETIEAYPEP
                     DFAKVMFLGLNAFTADLLFTRSQYYYGSHYVTDRTYPLLEQMTKVIMALNPDLKFVIR
                     FAEAAISSMRTPEAIESANSLLRLGAELYPDDYEFVFNQGYNYYIYLGEMEKAYPLMY
                     KGARMEGAPERLFWMVSRVTTMGGGYRLGYEYTKEKLSQTKDKNMKDQLEKELKHFEN
                     LIYLTEAVDKYIQEYQKSPDNDLTELIKKKIVKNIPADVFGGKYFFDDKDGKVKTTTD
                     GERYYLETQKRKREQELKELKSKEEPEILKSE"
     gene            complement(14478..15386)
                     /locus_tag="PL056_02905"
     CDS             complement(14478..15386)
                     /locus_tag="PL056_02905"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPM45998.1"
                     /translation="MLVSVFVPFANEENNLAELIERIEKGGIAAGVDLELVMVDDGSK
                     DNSYDIVMKEAQSRQWIKVFRHQRNKGLTEAMITGFRQCKGEIIIFLPSDLESHPDED
                     IPALLNGFEPGVDIVCGNRKKRREIKIFLSMLYNQISNFLFGIKLNDMNWIKAFRREC
                     LNDLELRSDWHRFLVQILHEKGYHAVEVPVKWYRRKSGRSHFGFRRIPISFFDSIAVK
                     FVLTFTKAPMRIFGAFGFLQLIGSIGIVSWMLYAQFVLGQPVFRIRPLLYFSIALFLS
                     GLIFVFMGFLAELIVGLRDEIRKMKE"
     gene            complement(15403..15699)
                     /gene="yhbY"
                     /locus_tag="PL056_02910"
     CDS             complement(15403..15699)
                     /gene="yhbY"
                     /locus_tag="PL056_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HPM45999.1"
                     /translation="MEKLTGKQRKYLRGVSHSLNPVVHIGKNGLADPVLKQIDDALSI
                     HELIKIKFIDFIDEKNEIAAEIEKKLKCESCGSVGHVFVFFRQNEEPEKRKIKL"
     gene            complement(15687..16625)
                     /locus_tag="PL056_02915"
     CDS             complement(15687..16625)
                     /locus_tag="PL056_02915"
                     /inference="COORDINATES: protein motif:HMM:TIGR01212.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HPM46000.1"
                     /translation="MSAKIDEMIFTFNRYMKKKFGGRVARISFDTGYACPWGKCVFCR
                     NDSFSPIVSVNMRKDNRHETLEKSMTFLKNRYNNKFFAAYFQSGTSTSGPVEKLREFY
                     RSAASVEGVVAFIVSTRPDYIDEEKIKMILESVPAHIDEIWIELGLQSVKEESLKWID
                     RGHSPDDYFQAVELIEKYGQGRIKVAPHIILGIPGETIEDMINTVLKSIENPVVKGLK
                     LHHLQIHRGTVLEKMYQENPFNLLTSDEYIKIMGEIIAVIPQDKVLFRLFTTAPEEYL
                     VAPIWNLGTQEALQKLENWLNINRITQGCRRLEWRS"
     gene            complement(16610..>17602)
                     /locus_tag="PL056_02920"
     CDS             complement(16610..>17602)
                     /locus_tag="PL056_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46001.1"
                     /translation="LVAFVKDNPGDDIFKTAKKILFFTENKGKFLKKCPGSKGVVCCD
                     YYTINSVTGCPYDCSYCILQHYIENNPFITVFLNREKAMDEIEEFLKNNSWLRVGTGE
                     LADSLALDHLLDESGFFLSEIEKRGLQNKIQFEFKTKSAEVDRLIEVFKQHKSVNTVA
                     GFSVNIPQFQEKEEPGAESINNRINAARLLIKEGINVAIHFDPVVMIDSFHDEYLKTI
                     DHIFSNLDCSKVVWISMGGFRHTLSLTETITKRFPESSLLLGEMFPGEKDNKLRYLAP
                     VRREFYKAFIDRISKYFNGNPPLYMCMEKSFMWSDMNMPLIKAVFPGGRNCLQR"
BASE COUNT         4592 a         4101 c         3230 g         5479 t
ORIGIN      
        1 tcgccatatt ccatttcggt tctctgtttg aattcatcaa aattttgaga atcataggaa
       61 attccggcag ttgaacggaa tcttttcaca acgagctcat cttcgacagc tatcgaaaaa
      121 tactgggcac ggagatctct caccggataa ggatcttcac ccattttttc aattatgccc
      181 ggagattcat ccattgatat cgcctgttca tgcgcagtga aaaattcatc tctgaaatga
      241 atcatcatac tcattttgta attgtctgaa aacttataag ccgggagaat tctgaacccg
      301 taacccatct gttttttaat atcaagaaaa gggtctgtga tcgctgtact ttttgagccg
      361 ttgaatgccg ccacatcctt tgaataaaga tgatcgttat ccgcataacc cttctgcgac
      421 tgataaacct gatagaacca gccggtgaat gtcatgtcga acttttctgt gttgatcgtg
      481 gcgtaagggg atattttata attccaattc tcaggataga caaatgaacg gttgcgcaaa
      541 atccagtcct gaacataccg cggatcatcg tgaaaccccg gtctgcgggc atttttccaa
      601 acttcctgag caggatcata atcgctgtag cagttgttct gataggtatt gctctttagt
      661 tcgctatagt gataatctcc gcttaatccg gcttcaaacc atttcaccgg gttccatccc
      721 gcttttgccg ctgcgttata ttttgtaaat tcagtatgat tccacttgcc agaatcttca
      781 atatactgat tttttgaagg gttcagaatt tcatcaaaag tgactccgta acggtcataa
      841 cgcaaaagtt tgttgtacca tttctttctt aaagtgctgt caagcttttg agacggttta
      901 aacccgtttg aaagaagaac actacctgaa aaccagtaat aaaaatcacc ggcaggcatt
      961 ccgtgtgaaa gagcgaactg ggaattattc catgagccgt attccgcttt tgagtccaca
     1021 aaaagtttgt ccggtttttt tgtgatgatg tttatcgagc cgatcgctcc gtcagttccg
     1081 tacagagccg atgaaactcc gcggtttacg actatctttg aaacagtgtt taccggaatt
     1141 gccgaaagat ctacatcagt tgaatatatg tcatttattg gaactccgtc tatcagaatg
     1201 aagatttttt cctgatcaaa ccctctcatt cgtatccttt tgtgcccttt tgtgtgggta
     1261 tagacctgaa ttcccggaac tccctcaagt gcctggtcga gcgttgaagc cccttttgcc
     1321 tcaatatctt cagcccggat caccgttgtt gtcgctgaat cctctattac ggcttttgac
     1381 ttgttccctg tgactacgat ctcaccggct ttaaaaactg ttttttccgg tttgccttcc
     1441 tcttccccgt tctcatctgc aaaaattgtg aaagaaaaca gcatcaggaa taaaaaaaca
     1501 aaccatttat tctgactact catcatagca ggcatcaagt ccgtcaacaa ctccgaaaga
     1561 gcttacatct tcaggagcta tgtcacagtt tttgtccatg ataaagaggc agtatccgtc
     1621 agcaaccttt cttctgtctt ttcctgaatt atcggcagag ccggttcctt cacagatcgc
     1681 atcgcattct gctgaacatc cgaatgaatt ttcagcgata tagaaatcgg cacatactga
     1741 aagtgcggca aaacagtcaa cacagccctg aattgcagta acttcatatg ttccttcgca
     1801 tctgaaccac tcttttacta tttcctgaca gcatagaagt gcttcttctg aaggtggcgg
     1861 aagagcatca tcagtcactt cttcgtcgtt ttcctcatca ttttcttccg actccgttaa
     1921 aacttcgtcg gacaagtcgt cagaaaactc atcgtcagca gtttcgtcag acgcttcgtc
     1981 gttttcttcg ttgtcaggaa ttattacatc atcaactgct tcgtcctgcg tttcatccga
     2041 ctccgttaaa acttcgtcgg acatgtcgtc agttacagtt tcattgtcgg caagtccact
     2101 gtcgttgtcg ctttcagctt catcgctgct gcatgaaatc gttgaaaaaa taagaaaaaa
     2161 gccaagaata aataaagata cgcttctcat gtcaacctcc ttttcaagtt tggaaggaaa
     2221 gcacttttca atgcagaccc tgtcggcacc ctttccatag ccccattgcc ttaggaaaga
     2281 gcactcggag aattatttag aaaaatattg aaaaacagga tgttgtcaag tttataaggc
     2341 gttatttata ttcgcaaaga tatgcggtat caactttgac tttgagctga aattttgaat
     2401 ctttctcaac tttgaatgtt gttccagcaa tgtattcctt ccattcgtca gaaccgggaa
     2461 gttttacagt cagtgctccg gcaacaaccg tcatatattc aactgttgaa gttccaaact
     2521 catattcgcc aggagccatg actcccactg tagcgttacc gttaccggga ttgaaaccaa
     2581 gtgatgtcac ttttccatca aaatactgat taactttgaa cattttatcc tccgctcata
     2641 aggtgaatga ccttcacttc ggctccttgc ggaaccagag ttgaaggcca ttcttcccgt
     2701 ttaattattt catcattcac atgaataaca agcagtttga aagtatattt catcacattc
     2761 aggacatctg ccaccgtcat cccttcagac cactcgatga catttccgtt gactgttatc
     2821 gttctttcca tctttcttac tgtaaaggtc ttacgacttc aataacttca ggaaggtcca
     2881 ttccaatatt tttgagatgt tctatcttcg gaacaccgtt ctttgtccag ccccttcttt
     2941 cgtaaactgc atcaagaagc tgttcatacc ggtcttccct gtattttctc ataacggaca
     3001 tcctttcctc aagcgatttt ccggcaggat caaaattcac aagttctttc aattgcttgt
     3061 catagaaagc ttctctgctt aaatattccg cttttgttac aggacctgcc gctctgtagg
     3121 gctgagcatc atgttttcta agaccgtaac cccttctgat attgaaaact ctctgaaaat
     3181 tgtaaaccct ttcagactgt cttatcaatt catgcttatc aatattgttg cctgtaacag
     3241 cattataaat aatcacataa ctttcaacat gctcgggaac ttttgccggc tcagctgtct
     3301 gacggttgtt ttccggttca atgtcattcc agggaagttt gcagaaaccc tgaagtccaa
     3361 accatgtcct gaacatcggg aaatagtgaa gcgcctcagc tttattttca aaagtcggga
     3421 tctgattatt gaccatatcc ataaatatca accatgcttc gtcgtgctga ggacctttgt
     3481 tggtcatggc atatccaccc tgctgagcaa gcgattcctt tgaaacatac tgggaatatt
     3541 caagaccttt attctccatt ccgatatcat tcatgaattt gatatcaccc caaccctttt
     3601 cagcaaagta attcttcata tatctgacac ctttgccggc aataagaccg aacccttctc
     3661 ctcttgccat ctgatggaga agctcaagag cagaggaact gtttccgaaa ttaagttcga
     3721 gtccgcctgt cctttcttta ttgagaatgc cgttttcata acattccatt acaaaagctg
     3781 ttgctgtccc tacggtaatt gtgcaaactc cgtatgtatc acaatagaaa tttgcctcga
     3841 tgatgtgatc cgcatcaaat attccgcaat ttgacccgag acctgcagca gtttcatatt
     3901 caggaccgtc aacaatgact ttctgacctt tgtaaggacc tgtcttgagt tcaaaaccgt
     3961 caaccgcctt tgcacagctc atagaacagc cgaaccagca gccctcaagg tttttccagg
     4021 tttgtgtgaa ctttgacttg aatacttcag aatggatatt tgctatctga gaatcgtctc
     4081 ttccatactg aaaatttttt gttggaagaa gatcgtactg gttcattatc tccatgagat
     4141 gggccgtccc tttggttctc atttcacact gctgatcatc aagcttcatt tctttattaa
     4201 aacgctgtcc tctttcctgg atcgctttca tatcggcaac attattaaga tcccctttta
     4261 agccagtgcc gatagcaaca agtgctttga tctttttatc tctgaaaaca cttccgatac
     4321 ctccgcgacc tgcctgttta agacggcaca gctttcttct cggatcgtaa aaactgaagt
     4381 taagcattcc gatgagagaa tgatctgccg cgcttccggc tgaaacgaca gctatgttta
     4441 ttttgtcctt ttcatccagt gcatacattt ctgtaagctg ttcggcaaga atgtgactgt
     4501 ccattgcttc cgccggagct tcttcaatag tgactttgtt agttctgccg tcaattatga
     4561 ttataacatc tttctgtgct ttcccctgaa gctcaatagc atcaaatccg ttgaacttca
     4621 tatatggacc gaaataccca ccgacattgc tgtccataat cgaatcggtc tgaggtgaaa
     4681 ttgaaacgac aagcgatttt cctgcgccgc tgtactgagt gatgcctgcg catggtccgg
     4741 ggctgataat tatctcattc tcaggatcgt tccattttgt ctccggcttt gttccgtccc
     4801 acagaagttt aagcccgaaa cccttgccgc ccacgaactt ttctttcatg tgctgagtca
     4861 ccggcttttc tgaaattgtg ttgtctgtaa gattgacata nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnnnn gaggcatatg aattttatcg tgatgccgcc gcaaagacaa
     5041 agaacgcaag cttgaaatca attttcaacg agttggcaag agaagaaatg ggtcacaaag
     5101 atcttcttga aacctacgct tttgatgaac ttagaactat taagttcaaa gaagttaaag
     5161 actacaagat cactgaatct gtcgaacttc ctaaactttc gactgaaatg agctttgttg
     5221 acggtatagt tctagcaatg aaaaaggaag aggaagcaat gaacatgtac agaaactttg
     5281 cagaagtctg tgccgacgat gcccagaaaa atgttttcct tgaacttgca aaaatggaac
     5341 agggacataa agcaaaactc gaaaacatct ataacgacac agcgttcggc gaaagctggt
     5401 aaatttaagc tttttctatc ttgcaggcac agactttgta ttcagggatc tttgcaacag
     5461 gatcaagtgc attattggtg agcttgttgg caggagcttc ccagaagtgg aaaggaacat
     5521 aaacggtccc tttcgggcat ctttttgtta tgaatgcctt tgttgttatt tctccccttc
     5581 ttgtgatgac cttgaccatt tcactgtttt taacacctat cgcttcagca tcttcaacag
     5641 atatcattgc gtgaggaccg gcaagattgt taagaccttc tgatttcctt gacatcgttc
     5701 ctgtgtggaa atgctgaagg atcctgcctg ttgtaagata catcggatat tcctcatccg
     5761 gaacttctgc aggagcttta aaaggaattg cagtcataag tccgagacct cttgtaaact
     5821 tgccgatgtg gagaacaggc gttccgggat gctcttcatt aggacacggc cagtgcattc
     5881 cgttgaatcc aagacgctta taagtaagcc ctgcatagct cggagtacac tttctgatct
     5941 cttcaaaaat atcttctgca catttgtaat tccagttctg tcccatttta ttcgcaagca
     6001 tctggtgaat ttcccagtca tgtttagcag taccaggagc tttaaccgct tcaaaaagga
     6061 gctgaaccct tctttctgtg ttggagaaag ttccactctt ttccgcataa gttgaagcag
     6121 gaaggataac atgggcaagt tttgcagttt cagtcatgaa aatatcttcg acaacaagga
     6181 attcaagctt tttgagcgct tcctcggcgt ggtgaagatc ggggtctgat atcatcgggt
     6241 tctcacccat gatgtagaaa gctttcatct gaccttcgta agctttgtca aatgcgacag
     6301 tcatgggcat accgatctta ttgtcaagct taactcccca gaatttctcg aacttttcct
     6361 gaactgcatc gtcgcctact ttctgatatg ccgggaaaac atttggaagt ccgcccatat
     6421 cacaggcacc ctgaacattg gactgaccgc gcaatggatt gacgccgccg ccgactttgc
     6481 ccatatttcc gcagagcatc tgaaggttcg ctgtggtctt aacattgtca acacctgttg
     6541 tatgctgagt gatacccatt gcatagtaaa gagccgctgt ttccgctttg ccgaaaagtt
     6601 cagctatctt ctcaagagtg gcagcaggaa cacccgatat tttctcaaca tattccggag
     6661 taaatttagc tacttcttct ttaagcgctt cgtaaccttc aactctctct tcaataaact
     6721 tcttatcttc ccagccgttc ttaatgatga gatgcatcat accgttcatg acagcaacat
     6781 ctgttcccgg tctctggcag gcataaattt ccgcatagtc cgccattctg atccttttgg
     6841 ggtcaataac tattattttc aactttcctt ttcttgccgc ctttttaaga cgggctgaaa
     6901 ttacagggtg cgcttctgaa gtatctgaac cgattatcag agcgacatca cttttatcca
     6961 taccggcaat gtcatttgtc attgcacctg aaccgaatgt agcagccaga ccggccactg
     7021 ttgagctgtg tcaaagtcgg gcgcaatggt caatgttgtt ggtacctatc acctgtctga
     7081 aaagtttctg gaatgtataa ttctcttcgt ttgtaacttt tgccgagcag agtcccatta
     7141 tcttgtcggc accatgtttc tccttgatct caaaaagctt tctggcaacc acatctatcg
     7201 cttcatccca cgatgcttct ctgaaatttc cgtcaggatt tttaatgaga ggagttgtca
     7261 gacgatcagg atgatttaca aaatcgtatc cgtatctacc tttcacacaa agcattccgt
     7321 cattggttga agcaccttcg acacccttta ccctgattat tttgtttgct tttctgtcaa
     7381 catgaagctc aagtttgcaa ccgactccgc agtatggaca gactgaataa accttttcga
     7441 tttcccagta ccttgcaaga cctttagctt tcttatcggt gatcgctccc accggacaga
     7501 tctgcgaaca ttcaccgcac tgaacgcatg atgatgtccc catcggaaga tctgcgtcaa
     7561 atgcgatatc tgctttgctc cctctgtact tgaaatcgag aacttcgtta acaactgttc
     7621 cgttacaggc agcaacacat ctgccgcagg agatgcattt gtttctgtca acatagacaa
     7681 attctgaact ttcatcaatg ttcttttcta ccattccgta attgaaagag cccctttcaa
     7741 tgccgagata ataagcaacg ctctgcagtt cacagcttcc attctgatca caggaaatac
     7801 aatcgtgcgt tcctgttgaa agaagaagat ctacaataag tttctgtgaa tttacaactt
     7861 tttctgtttt tgtctggatc ttcatccctt ctctgacttg aacagagcag gatgtctgaa
     7921 gaccgttcat cccttcgatc tcgaccacac agacacggca tttgcctgct gcaccgacct
     7981 tatcatggta gcagagtgtc ggaatataaa ttccggcatc ccttgccgcc tgatggatgg
     8041 tctgtcccgg ctttgcttca actgcaattc cgttaataaa aatctgcatc aaaacctcca
     8101 attattttat aagtgcttca aattctggtc tgaatttttc aaccattgct ctgatggggg
     8161 tcgagaatgc ctcgcctacc ggacagagcg taagtccttt gatcgtcccc acaagccgtt
     8221 caacatttgc aagatcttct tttgttccct gacctttaac gatcttttca aggatcctga
     8281 ccgtcatttt cgagccctct ttgcagggag tacattttcc gcaagattca tgtgcataga
     8341 atttcatagt acggagagca agttcaggaa ttgaccattc gtcattgatc accattattc
     8401 cgcctgaacc gagtgccgtt cctttagcgg cgcatgatgt atagtccatt ttaaggtctt
     8461 caagttcttc ggctttgagt atggcaactg aaagtccgcc gacgattgcc gctttgagct
     8521 ttccgtcaac tccgcctgcc gcttcaatga gatctttgaa cgatgtacct ataggatatt
     8581 cgtaacatcc cggttttttt acatttccgc tgattccgaa tattttgaaa tcaactttcc
     8641 tgaaagcgtc agcaccgttt tcgatgatgt atggaacgct tgcaagagtt tcgacattgt
     8701 tgacgattgt cggacatccg taaagaccct gaaccgcagg gaacgggggt tttattctcg
     8761 gctgacctct ttttccttct attgattcaa tgagcgctgt ctcttcaccg catacatagg
     8821 cgccggctcc gcgatgaaca ataatatcaa ggtctttcaa ctgaccgttc ttttttgctt
     8881 cctctatcgc cctgtcaaga atgtctgcta tccagtcaaa ctctccacgg atatagatga
     8941 atgaaagttt ggaaccgagt gccagcatcg aaattgccat cccttcgatc agaagatggg
     9001 gatcatattc catgatctgc ctgtccttga aagttcccgg ttcgccttca tcggcattac
     9061 agatgagata gaccggtttt cctgtatctt tcggcaggaa tccccattta agaccagtcg
     9121 ggaaacccgc accgcctcta cctttnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9241 nnnnngcaag aattttgcct gcacccatca aagttgccgg gatatttgta tcaacctgaa
     9301 tatggtattt accgatcttc tccctgacat tgtacattgt gtaaaatgtt gcaactccga
     9361 atgccttcga atgtgtgaca cctaaaattt cagctacacc ctcaatatcc ttttttgaaa
     9421 gagcattgcc gttttctttc tgcacgatca tcaatgcagg aagtatggcc gattcctttt
     9481 tcggatagcg tgaagccaaa tccttgattt tgttaagaga attttcggaa atcattaatc
     9541 acctcactaa gaaaataaaa cttataaagc tcagcatcga ccgagtatat ttaaaaaata
     9601 acggcttttc aaccttttat atgaatattt tataatatat ctgagtttat tgacttaacc
     9661 ttttttttcc tttgaatgag accatttttt tcaaaaagtc ggtatatctc tggaaacggt
     9721 ttgagcgctc tgggaaaaac tccgtgaaga taactgatga ggactccgta attggtcatg
     9781 gggatctgcg cttttgcagg aattgactgt cttctcagca tttcctttct gttgagtgtg
     9841 caggcaccgc aatggacaac cagtttatat tcatcaagat tatcgggata ttcacatccg
     9901 ggaactgttt ctattttcag tccgcctccg acatgggcat tgagccatct tgggatcttc
     9961 acccttccga tatcatcttc cagcggatgg tgtgaacagc tttccgcaat cagcactttg
    10021 tctcccggtt tcagattttc cacagcaagt accgctttca ccatctcggc aagatcacct
    10081 ttctgcctga ttgaaagtat ggaaaatgaa gttaatggaa cactttccgg aacatctgct
    10141 gaaacctgtg aaaaaacttg agaatctgta acaaccagag ctgggggatt cttcaatgaa
    10201 ttcagtgtag taagcagttc tctctccttg cacataacgg caacaccatc accatcaagt
    10261 atgtcgcgga tcgcttttac ctgcggcatt ataagacggc ctttcggtgc gccaagatca
    10321 ataggggtga ccagaacgac cgtttctccc ggatgaatga gatcttttaa aaaaggcggc
    10381 tcccattctg aaggtgctac ttcgattatt ctgcttttaa gctcggcaat tcctgtgttt
    10441 tccttggcac ttacccttat aaaacttttt tcaactatcc agttacggac tgattccgat
    10501 agacctttat caattttatt aagaactact atcaccgaac ttttgttttc gccgcactgc
    10561 ctgataagat catcttccat gtcccctgcc gttcctgctg aatcaacgac aattatggca
    10621 agatcggtct ttgaaaggac tttttcagac cttttgaccc tttcttcacc aagcacatcc
    10681 tcaagatcgt ccattccggc agtatcaata agcacaacag ggcctatcgg cataagttcc
    10741 atcgacttaa agaccggatc agtagtagtt ccgggagtct cagaaacgat tgaaacctgc
    10801 tgatttgtaa gtgcgttaat taatgatgat ttccccgcat ttcttctgcc gaaaaatgca
    10861 atgtgcagcc ggtcaccgcg aatcgttctt gaattcattt ttattcctca aataactttt
    10921 ttcaaaggga ttatattttt tacttccttt cataaaacac gattccgatc ctgttaatgt
    10981 gatcaacaac ttcagggtcg ctgatttgat gaaaaattga aagatcgaat gaatatggga
    11041 gaaaaaggtc gtcaatgtct tccatgattt tgtaaagttt ttgaagcgta aggttatctt
    11101 ttccgacaag tgtgagatca atatcagagc cgttcctgaa attccctttt gcccgtgaac
    11161 cgtaaagaac cgccttttca acttcaggat gttccgcaaa aactgaacag atacgactga
    11221 tcacatcttc tttaagtcca aatctcattt tatccttttt cagcaatgtt tctgagcact
    11281 ttcgaaagct cttcaaattc agtcagataa tctttaacaa tcgcctgagc tattttttcc
    11341 gctatttccc tgttatatgt gtgggaacta agatttctgc ttttgatcat atccatccac
    11401 acttcaccgt ttgcgataag tcctctttta aatgcttcac gggtggtatc ctttgatccg
    11461 taaagactat gatgcccctg atcttcaaga aaatccttga tcgtgttcca gcccagttca
    11521 tgagtatatt caaatgactg gatcattccc tgctcttcaa gctcagtcag tttgcggctg
    11581 tttgcaagct cgacagcttt tttcaattga aacagtgcct gttcaaaatg tttgaaccgc
    11641 tgaatccacc ttatgtcttt attactcata taatctccta ataaaacagt ttgcaaaaaa
    11701 gaataacctt atcgagtttt ttttcaagtc cagaacaatc aataaccatt ttattcctcc
    11761 acaacaggca ttccgggact tgccatgact tcaggtgtca ggagcatgtc gagtttttcc
    11821 tttgagagta ttttcctttc gatgatgaga tcataaattt tcgccccttt ttctcttgct
    11881 tcaacaacaa gctctgccgt tctttcatgc ccgatcgttg gtgcaagaag tgttgcaacg
    11941 actgttgaag attccacata actccttatt tttgtttcat tagctgtaat ccctgagatc
    12001 attttcgtga agcttttcaa cattcctgaa aatagcgata ttgaatgaac ggtcttgtgg
    12061 gcgatgaaag gcatgaactg ctgcaactca agatttccct gagaaactgc atgagcaact
    12121 gaattgtctg atgccattat ttcaagacac acctgttccg ccgcctccgc cattaccgga
    12181 ttgacttttg caggcataat tgacgatccc tgctgaaaag ccggaagaga taattcagct
    12241 attccgcact cgggtccgct tgacaatatc ctgatatcac ttgcaatttt cttgatgact
    12301 actgcacctg ttttcattat tcccatgatc tcaactattg aatcgcagtt ctgagttccg
    12361 tcaactccgt tccttgaaac ggaaagcggt tcctgaacca ccttcctgag ttctgaagcg
    12421 atgacccgaa gataatcggc atctgcattt attcctgtgc cgacagccgt tccgccgata
    12481 ttgacctctc ttatccagtc aagcgctttg gaaagccgcc atctgaacct tgacactcct
    12541 tccgcccaag ctccgaattc ctgacccaga gatacaggca ttgcatcctg catttccgtt
    12601 ctgccgatct tcaaaatacc tgagaactga tgctcttttt cctgaagttc atgcagaagt
    12661 ttctcaatat tttcatgtag ttgcctgatg cctcttattg tcgctatttt aacagccgtc
    12721 ggaaacacat catttgtcga ttgtgacagg ttcacatggt caagagggtt gataaattca
    12781 tattcgccat agtttttccc aatgatctta agcgcatagt tggcaataag ttcattggct
    12841 gccatgttca ttgaagttcc cgctccgccc tgatacgggt tgagatgagg ataaatcgat
    12901 tctgcgatcg cacggtcaca gccatcttct attgcagacg cagtatcttc atcaagtatc
    12961 ccggcaatcc tgtttgaaac agcacacgcc ttttttactt ccagaagtgc cttaaaaata
    13021 gatggatgca tcacttcatc attaaaattg aaattctgca tcgctctcat tgtgtttatc
    13081 ccgaaaggaa aactgtcatc cagctccaca acacccataa aatctttctc tttcctcatt
    13141 ttatatccct aaatctaaaa tccgtcaatg atcatcttta tagttgtata aggtattatc
    13201 aatcagagaa aaaataaaga ttttaaatat gatctcaaac ctttttactt gacaaagagg
    13261 gcctgcaaat gttaaaaaga gccgttgact agtccattat taacttgatt ttaggagaag
    13321 agaatgcaca agatcaatgc cagcctttgc actaactgcg ggctttgcgc ttcagaatgt
    13381 cctgttgaag cgatcagcga aaatggtggt gtccatgtta tttccgatgc gtgtgttgac
    13441 tgcggcgcat gtgtttccgt atgtcctgtt gaagcgatca gcggctaatt atagtttctg
    13501 aaaaattttc agacctccgc aagggggtct ttttttttgc ttgaatttta ttctgacttt
    13561 aagatttcgg gctcttcctt gctttttaac tctttcagtt cctgttccct ttttcttttc
    13621 tgtgtttcaa gataatatct ctctccgtca gtcgtcgtct ttacttttcc atccttgtca
    13681 tcaaagaaat attttcctcc gaaaacatct gcgggaatat ttttaacgat ctttttcttt
    13741 atcagttccg taagatcatt atcgggagat ttctgatatt cctgaatata tttgtcaacc
    13801 gcctcggtaa gatatatcag attttcaaag tgcttaagtt ctttttcaag ctgatctttc
    13861 atgttcttat cttttgtctg tgaaagcttt tcttttgtat attcatatcc cagacggtag
    13921 ccacctccca tcgtggtaac acggctgacc atccagaaaa gccgctctgg agccccttcc
    13981 attcttgccc ctttatacat cagtggatat gccttttcca tttcgccaag gtaaatgtaa
    14041 tagttgtatc cctgattgaa aacaaactca taatcatcag gataaagttc tgcacccaga
    14101 cgcaaaagtg aatttgccga ttctatcgct tcaggtgtcc tcattgatga aattgccgcc
    14161 tcagcaaatc tgatgacaaa cttcaaatcg gggttcagcg ccattataac tttggtcatc
    14221 tgctccaaaa ggggataagt tctgtcggta acataatgac ttccgtaata atattgggat
    14281 cttgtaaaaa gaagatctgc cgtgaaagca ttcagcccaa gaaacatcac ttttgcaaaa
    14341 tcgggctcag gataagcttc aatggtttca actaaaggtt tgtcattttc cataaggtgc
    14401 tttctcacat gatagaacgg atagatcacc gccaacaaca ggagaaatgc aataagtgtg
    14461 gaaagtttct tcatttttca ctccttcatc tttcttattt catctctcag ccccacaatg
    14521 agttcagcca gaaaacccat gaatacaaat ataagccccg aaagaaataa ggcaatactg
    14581 aaatataata aaggtcttat cctgaaaacg ggctgcccga ggacaaactg cgcataaagc
    14641 atccaactca caatcccaat agatccaatg agttgcagaa aaccaaatgc cccgaaaatc
    14701 ctcatcggtg ccttagtgaa tgtaagaacg aatttaactg ctatcgaatc aaaaaatgag
    14761 atagggatcc ttctgaaacc gaaatggctt cttccgcttt ttcttctata ccattttaca
    14821 ggcacttcaa ccgcatgata ccccttctcg tgcaggatct ggacaagaaa tctgtgccag
    14881 tcgctcctga gttcaagatc gttcaggcac tctcttctga atgcctttat ccagttcata
    14941 tcattgagtt ttatcccgaa aagaaaattg cttatctggt tgtaaagcat cgacaggaag
    15001 atctttatct cccttctttt ctttctgttg ccgcaaacaa tatcgacacc cggttcaaat
    15061 ccatttaaaa gtgcaggaat gtcttcatcg ggatgacttt caagatcaga aggaagaaaa
    15121 atgattattt cccctttaca ctgcctgaag ccggttatca tcgcttcggt aagccctttg
    15181 ttgcgctgat gtctgaaaac cttgatccat tgtctgctct gagcttcctt catcacgata
    15241 tcataactgt tgtcctttga tccgtcatcc accatgacaa gctcaagatc aactcctgcc
    15301 gcgatacccc ctttttcaat tctttcaatg agttcggcaa gattgttctc ttcatttgca
    15361 aaagggacaa ataccgaaac aagcatcatt tactccagtg atttaaagtt ttatctttct
    15421 tttttccggc tcttcattct gcctgaaaaa aacaaaaaca tgacctactg aaccgcagct
    15481 ttcacattta agcttttttt ctatttctgc tgcaatttca ttcttttcgt caataaaatc
    15541 aatgaatttt atctttataa gttcatgtat gctcaatgca tcatcgatct gtttaagaac
    15601 gggatcagca agtccgttct ttccgatatg tacaaccgga tttaacgaat gggacacacc
    15661 tcttaaatat tttctctgtt ttcctgtcaa cttctccatt ccaacctcct acatccttgc
    15721 gtaattctat ttatattaag ccagttctca agtttttgca gtgcctcctg agttccgaga
    15781 ttccaaattg gtgcaacaag atattcttca ggagcagtgg taaaaagccg gaaaaggact
    15841 ttatcctgcg gaataactgc aataatttca cccattatct ttatatattc atcacttgtc
    15901 agcaaattaa acgggttttc ctgatacatt ttttcaagaa cagttcccct gtggatctgg
    15961 agatgatgaa gcttcagtcc tttcaccacc gggttttcga tgcttttcag aaccgtattt
    16021 atcatatctt caattgtttc accgggaatg ccaagaatta tgtgcggagc gaccttgatc
    16081 ctgccttgac catatttctc aattaattca actgcttgaa aataatcgtc aggtgaatgt
    16141 ccccggtcta tccatttcaa ggactcctct tttactgact gcagcccgag ttcgatccat
    16201 atctcatcaa tgtgggcagg aacagattca aggatcattt ttatcttttc ttcatcaata
    16261 taatcgggtc ttgtggaaac tatgaatgca acaacccctt caacggatgc agccgatcta
    16321 tagaactccc tcagcttttc aacaggtccg gaagttgaag tcccgctttg aaaatatgcg
    16381 gcaaagaact tgttattgta acggtttttg agaaatgtca tgcttttttc aagtgtttca
    16441 tgcctgttat ctttgcgcat attgacagat acgatcggtg agaacgaatc attcctgcag
    16501 aaaacacatt ttccccatgg acacgcatat ccggtgtcaa aagatatcct tgcaactctc
    16561 ccgccgaatt ttttcttcat gtagcggttg aaagtaaaga tcatctcatc tatctttgca
    16621 gacaatttct tcctccggga aatacggctt ttatcagcgg catgttcata tcactccaca
    16681 tgaaagattt ttccatgcac atgtaaagcg gcggatttcc gttaaaatat tttgagatgc
    16741 ggtcaatgaa agctttataa aattctcttc tgactggcgc taaatatctg agtttattat
    16801 ctttttctcc tggaaacatt tcgcctagaa gcagcgaact ttccggaaat ctttttgtaa
    16861 tggtttcagt cagcgacaaa gtgtgtctga atccgcccat gcttatccag acaacttttg
    16921 agcagtcaag atttgaaaaa atgtgatcta tggttttcaa atattcatca tggaaagagt
    16981 cgatcatgac aaccggatca aagtggatcg ccacgttaat cccttctttt atcagcaatc
    17041 ttgccgcatt gattctatta ttgattgatt cagctcccgg ctcctctttt tcctgaaact
    17101 gcggaatgtt cactgaaaaa ccggcaaccg tgttaacact tttatgctgt ttaaaaactt
    17161 caatgagccg gtcaacttcc gctgattttg tcttaaactc gaactgaatt ttattctgaa
    17221 gtcccctttt ttctatctca ctaagaaaaa aaccgctttc atcaagaagg tgatcaaggg
    17281 ctagagaatc tgcaagctca cctgttccta ctcgcaacca gctgttattt ttcagaaatt
    17341 cttcgatctc atccatcgcc ttttcacgat tcaggaacac cgtgataaaa ggattgttct
    17401 caatgtaatg ctgcaagatg cagtaagaac agtcgtaagg acaccccgtc acgctgttaa
    17461 tcgtgtagta atcacagcag acaacgcctt tgctccccgg acattttttc aggaattttc
    17521 ccttgttttc agtaaagaac agtatctttt tcgccgtctt aaaaatgtca tcgcccggat
    17581 tatctttcac aaaagcgaca ag
//
LOCUS       DAOOYB010000029        17596 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_3131_length_17596_cov_9.846474, whole genome shotgun sequence.
ACCESSION   DAOOYB010000029 DAOOYB010000000
VERSION     DAOOYB010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 17596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17596
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3131_length_17596_cov_9.846474"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1807)
                     /locus_tag="PL056_02925"
     CDS             complement(<1..1807)
                     /locus_tag="PL056_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46002.1"
                     /translation="MNKKMLLLLLIALIFSVSCKKEKKPVSDDISDEDLLVFDEDTVD
                     SETVQDESDPLIDEEESDDLSDEGLTDSDEDISDEDTFVPLRFYVNLNATGANTGLSW
                     KDAFVKLQDAIDLAEEGYEIWVAKGTYKPDRVVKEITPVSNPGDEGYERFFHFSLKDK
                     VAVLGGFTAGETEKDQRDPAENETILSGDIDEIAGLSSGDAYNVMLNINIYEDTVLDG
                     FTITGGNADYSLSPGVDLYPNQGGGINNRSTANPTIRNCIFKGNYALIAGGAMANLKS
                     HPQIYDSVFEENNSARGGALSNDISSPYVQNTVFRNNSAPDGYGGAVFNKGKSRGFFI
                     DCRFTDNSANDGGAMNNTVDSGVTVTGGIFSGNEAHNGGAVSNSQSSPYFSDSIFENN
                     TASVGGGAIENFSDASPKIVNCIFKNNSATGISDGGAMLTNTGTPIVVSSLFYQNTAY
                     NGGAVANTGSSGHFIANTFVNNSATGQFGYGGGMYNQINSNPMITNCIFYFNSSENEG
                     NEIFNNSSDPVITNSDIQNSFFDTVWDDSLGVDAGGNFETDPLFTDMLYLDFSLKSPS
                     PCIDKGTNAPFETGGMAVSFSEDLDGNERISNGIAD"
     gene            complement(1831..3315)
                     /locus_tag="PL056_02930"
     CDS             complement(1831..3315)
                     /locus_tag="PL056_02930"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPM46003.1"
                     /translation="MSFFSQVKGFQKNYWIVNVMEVFERLAFFSVRAVLPLWMVATAD
                     QHGLGLTFTEKGLIFSVWAACQSLIPMFSGSFTDNFGYKRSLYTAFSLNIFGYILMAN
                     ATGFWTMMIAGITVGTGTAIFKPPVQGTIASSVNEENSSLGFGIFYWIVNIGGFIAPL
                     MASALRGNNTDGYTWHFVFYAAAVATAVNFIPTIFFYKEPAAKTGRSAKQALQNTLTA
                     LKDKDFMIFLMIFSGFWLMFMQLWDLLPNFIDQWIDSRDLAKLLPAFLTDHGSVKAEQ
                     IININALCIILFMVPWSIITGKFPRLVAITAGIFVTTFAFLTAGLFMAGTITAICIVF
                     FSFGEMTCSPKFSEYIGVNAPADKKALYMGLANIPFALGWIVGNMVSGPLYDAFANKL
                     IITRQYLLEVKRVPLDTLKDIVEKYKGSNPDKLLPETIEKMNIETFNFFPYFQQIMGV
                     DQYEANHILWEAYHPWRIWVILSTFGFITIISMLAYSFNKRKNR"
     gene            complement(3316..4734)
                     /locus_tag="PL056_02935"
     CDS             complement(3316..4734)
                     /locus_tag="PL056_02935"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HPM46004.1"
                     /translation="MKRFVFMIFITLFLANVYGELYEISIPRYDQKSKYLVSFLDIIS
                     AAPSEGTGLKYVVDSSPGEIALLYGNCVIKEMNRSITIFPEYSDLTGINKRIDRVVER
                     SGGKAEVFDIGLSIEGRNIRAVRISKNEKTDEDLPEVLFVGLHHAREWISSEVTMGIL
                     EFLVENMDSNPFVSDILQNSVIWVVPMLNPDGYLFSWEEARMWRYNRRVHPDDSIGVD
                     LNRNYDSSWIKIDYVHGEGPFSEPETLAVKNLILNDFEEPLADGIKSLDGLITYHSYG
                     QIIMYPPGSTEEPAAKHEYYKDLAEKMAYLAFSECGSVYIVMQNSSLYFTFGEMTEWF
                     MNTHDGKPSFTFELRPHMGSDYFFMLPEDQIIDTVKENITPALYFVRHIVSGDTDLVM
                     DIDRNGVNDVLENTSFDYKCDRSDLEIPDAVNDSESNDHSLDIPDSDNSDMTDTNGTD
                     NEQNDTVPAEDNGCNLTLIQGH"
     gene            4854..5318
                     /locus_tag="PL056_02940"
     CDS             4854..5318
                     /locus_tag="PL056_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46005.1"
                     /translation="MLEKDKTQYIVEKRRKLILSLVLVTVFGSVLYISGIIHKQDENK
                     EESASGGLFDIAKKLENEVSESAGKKMTSENLLKAPVSLLYSIENDKIKLKETFRINK
                     NVITDGVFTFDTHGDTIYSTGFISKERHVNCKGVMKTGNDRFFIYFCTEKTP"
     gene            5438..6877
                     /locus_tag="PL056_02945"
     CDS             5438..6877
                     /locus_tag="PL056_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46006.1"
                     /translation="MRRILVLSLFMMFIFPLYADKVLFMRPVTKEIEKSDAIKIVRDL
                     ESVLNSRFNSVSYKDVPKGKLAKLAKCGMKTECWYDNASDDDFQYALLSLISMNEDEE
                     ITIRFVFINIEYEEILDDSSKTYDYIDSVNEKEIYRQLRKTTSSVFDEIKSGTGSRKS
                     AKRDSRKSRSYSRDSDYEEDEDNEISARKEKAAQAEEERRRREEERRAKLEEERRQRE
                     LEIEKRREEEDRRRKELERKKRIEEERKRREEEAERERLEKERERREEEKRREQESKK
                     QTLMDNAEKLARARELVLEMCSQGKYNAAIKAIVKVSEIKCECEEDAKVLALKTQLLN
                     FNKMRDKILEGVKLLDYSLILDNLEAAKALDEYIVPGGTEFSKRIDKIEAVGYMAKGR
                     DMEKKDNYILANEAFEKCADLDSEKTECREWLDSKDKLVKRIYDKASVMKSFNPTKAK
                     ELLRSILKLVTADNEHYKKAEADLQKMEY"
     gene            7033..7839
                     /locus_tag="PL056_02950"
     CDS             7033..7839
                     /locus_tag="PL056_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46007.1"
                     /translation="MISISEGEDPEKFFLLTEKGLKKINFDIKEGFPFAYDIKKNMCG
                     LKKSDMDGFLIKIFDVKDNKNIVETSLPETVSSIFSGCFLKNGSLAMLLLEENENLSR
                     RYFLSLSEGLDINSWRSYLIREETSSDLIGKILSLGSSFERPVSIQCSDDRIFIHTLT
                     TYSDMIQASIYRFDVEKKALVFETGYHPLNVKDGTVSIFFNEKKNTGYVHQKRELVIL
                     KGSDFPVKTKFDEPGEVFFSPASDGEGLLIYFIPETKDHVPAKARIMQFD"
     gene            complement(7840..8658)
                     /locus_tag="PL056_02955"
     CDS             complement(7840..8658)
                     /locus_tag="PL056_02955"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HPM46008.1"
                     /translation="MNKVILKTVGFFKLYRPDVPVIVFLGTFAGRVFTTGFSVFVIFE
                     ALFLSLFPYNFVYTLNSITDVTEDSINKPGRPIPSGLITKNEALIWLLFLTVISVSGI
                     SLIFKGVEMFLAFLIILLGFSYSMPPLVIKKRALLAPVITGWGVTHPLFITGGTALFL
                     FSSSVMLHAIGTTSLKDLTDIEGDTAAGRKTISSTKGIPSAITMSLIFKISSIILFQF
                     TQYKIASIIPLLSIFVVLHHYFYLRKDFVRVIYKRTIWTTAALSVFVIAYVQLF"
     gene            8777..9736
                     /gene="rsgA"
                     /locus_tag="PL056_02960"
     CDS             8777..9736
                     /gene="rsgA"
                     /locus_tag="PL056_02960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HPM46009.1"
                     /translation="MGVIARVTGHGRHYYTVLSEKGEFKAKLAGSLRYNSVFKSDLPA
                     VGDMVEISLRGDKAYILSVFERKSCFSRRSSGNVIDEQVLAANLDILFVVMSLDENYN
                     LKRLQRYISAARAGNIKPVVLLSKLDLCSDPDHLTQQVRNMAEGVEVFAVSSANRETL
                     EIINMFLVTGTIAAFTGSSGVGKSTLINMLLGEERQKTAEISSAVKKGRHTTSSRDMI
                     FLNNGAIVIDTPGIREIQMWETEVPEGFEKILELAAGCRFSDCIHDTEPDCAVKKAVS
                     EGLLSSGILEMWRIQQVEMDELQQKKEESVKILESRKAKKRRS"
     gene            9736..10668
                     /locus_tag="PL056_02965"
     CDS             9736..10668
                     /locus_tag="PL056_02965"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MORN motif-containing protein"
                     /protein_id="HPM46010.1"
                     /translation="MSSMRKSFAATILFFFLCACATFEEKPDTEKMAELESMKIKKVE
                     PENKEDPIVDHGRHVLINGDTYIGGFRKGKMEGEGEYLYRNGNRYKGIFKNDEMDGAG
                     TFTYKDGTVYKGDFSEGKKHGFGEIIFRNGNWYKGTFYYDIIDGEGTYFYKNIGEYKG
                     TFINGKREGKGVMKFNNGSVYEGDYLKDRRTGKGKLTLADGTVYEGDFFNGAKQGKGT
                     IVFSNGDEYTGDFKKDLFHGQGTYKFATDGRVYTGEAVLGKLNGPGVITWPNGDKYDG
                     EIKDGAPHGIGTLYKSDGEEVTAEWAQGKIIFSK"
     gene            complement(10672..13038)
                     /locus_tag="PL056_02970"
     CDS             complement(10672..13038)
                     /locus_tag="PL056_02970"
                     /inference="COORDINATES: protein motif:HMM:NF015347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BspA family leucine-rich repeat surface protein"
                     /protein_id="HPM46011.1"
                     /translation="MKRLLLMAILFTFISCSLEKFERPETASEEDTTDNTDLEHDLTD
                     DPFLPDSDIEADDEYTAEIEGVVCTGQTKCYDDNTEIECPNENSNYYGQDGQYSTDNL
                     CVSKSFSIKDNGSGPVAIDNNTHLEWQQMFYLRKYEWDQAVENCKDLNYGGFDDWRLP
                     TYEELWTIIDLGAEESPAINTDVFPFTPADSFWTSSEDPLDNEIGWTVDFIYGGTRER
                     YKNSSHYARCVRGPKLSLNNNFSNKTIRNDRIIVDSTVNLIWTDTQFTNKNWKTALMT
                     CESLNYGGKANWRLPNISELKTLVNITKQDPASDMSGITSQKLWSSTTDSMYYDFYGL
                     TVDFERGTIEGTVKTENADVLCVTGVNDTNNRTCFSDKGCPEERSCIGGKCALPDSFI
                     SKWTTTYDGYSDENSIKLPLVDNGTYNFTVYWGDGTENKITKWNDPALLHRYNKKGTY
                     TIVIEGDISGWQFCNFNETEEKCENSDSGKITEIIQWGTFSFGKTEFQFSGCYNLEIK
                     AVDLPDLSETESLRGVFQDCYILVGTDFFKNLNVSGIKNMGDMFNNARIFNQDISGWN
                     VSKVTDMNSMFKGAVSFNQDISGWDVSNVADMGHLFSGAKMFDHDITGWNVSKVTNMS
                     GMFNGHESFNQNISGWNVSKVTDMNSMFKGAVSFNQDISGWDVSNVTDMSFLFNGAIS
                     FKGDLTTWNVSNVVNMYKMFSHAYSFDQDISGWNVSNVKYMQEMFYNTTLSTANYDAL
                     LNGWSKLILQNSVEFHGGNSKYSSSGETSRAKIISDFNWTITDGGLTE"
     gene            complement(13140..15281)
                     /locus_tag="PL056_02975"
     CDS             complement(13140..15281)
                     /locus_tag="PL056_02975"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HPM46012.1"
                     /translation="MKIQMLILITMFQTPFLTIADEKHDVEIVEDEETEIEEKKKEEE
                     KETVEDSTEEEIVVTGTKTEKLVSEAPVKTSVVGKAEMEKKNAANLADAIDNTTGVRV
                     ENNCQNCGFNQVRLNGLDGHYSQILIDGKGVYSSLAGVYGLEHIPVQMIERVEIVKGG
                     GSSLYGGSAVAGVINVITKKPSENFGNITLETQFIPSESGVDGLSKGLRLGGDAGLVT
                     KDKKAALFVYGGGLARQPWDENDDGFSELGETRQSFGGSNAFLNPFKGGELQGKFSIM
                     RDSRRGGDHFLRAKHKSEVAESTDTDRWGGEVKWKHEILSTKTDYEMSYGFAYTERSS
                     YYGGDDQFGDMYGKTKNPMHVLDASINQLANFLGVMMFTGGFQYTHEDLDDYTADGLA
                     STDETYSNIGGLIQIDWMPKKWVEIIMGVRIDKHSELDVPVFSPRATLMFSHRDFKSR
                     TSFSTGFRAPQIFDEDFHITVVQGEPSPTVNSDDLNAEKSWNISQQFEYKFTKNGFSI
                     KPSINGYLSQIYDAFNTKDIEESDSNYEEGINKKIRYNSGTTTSMGIELDIEMAYKKL
                     WTLDIGWTFEKAENSKNDEDLAGVGINEKRVLKTPNFYGYIAAALTPWKGLEFTTALN
                     LTGPMKIKHEEDFNGSDFELTTSDWFADWNIGIGYKFKADSYSVKPYFTVKNVLNSFQ
                     DDFDEGAGRDAGYIYGPRSPRTFVLGIKGEI"
     gene            complement(15296..15973)
                     /locus_tag="PL056_02980"
     CDS             complement(15296..15973)
                     /locus_tag="PL056_02980"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HPM46013.1"
                     /translation="MNSDRIFALAVILSIIFHGVLIASKTDLFKESSIVTTTKILLNF
                     EEPPPAPPPPPPPPPKPKPKPKIKKEHVIEKPEELREEITDVASPSDGLRMAEMVDAK
                     AGRYEAGIGKPEEPQKPAEKKVDTKKILSEYTKKVNTEIARNKIYPSFAQRYNIEGVV
                     KISFLIDTDGTFKEIKITKSSGQEILDEAAFESVKKSSGRIKKPVEIGHLKIKRNIII
                     RYELTNS"
     gene            complement(15963..16400)
                     /locus_tag="PL056_02985"
     CDS             complement(15963..16400)
                     /locus_tag="PL056_02985"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HPM46014.1"
                     /translation="MIKFSQNLKRKSNREVNLTPMIDIIFNLLIFFLITAVISQKGLN
                     IKLPSAATAEKRPPKALEITVTKDNVITFNGTVVEKDNVLKELKKEKELATNEKHDVI
                     TLKADETAHFGLFIFVMDTARKLGFENLAIAAEKKAENEINEQ"
     gene            complement(16397..17008)
                     /locus_tag="PL056_02990"
     CDS             complement(16397..17008)
                     /locus_tag="PL056_02990"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HPM46015.1"
                     /translation="MMEFIEKGGFMMYPIILSSVVALAVFVERMFVFSTIKMLNKENL
                     REIFNSIEQKGVKETLSMIESEKGIMSSFLTAILKEQSEEQCEKAASTAGDDILFNLN
                     RRLDLLSMLGTVTPLMGLLGTVVGMIKVFSRVATAGDMSDISILAGGIWEALLTTAAG
                     LAVAIPILFAHNYLERKVDKISHRMMQKAQKIVSLMRNSGAFR"
     gene            complement(17056..17469)
                     /locus_tag="PL056_02995"
     CDS             complement(17056..17469)
                     /locus_tag="PL056_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46016.1"
                     /translation="MLKRVRFVTLFILSIFYVLDGYLAILHFSNITHKFSQEDQAFVD
                     VYFNWKAASNYHHNSEEGIFDPYSFFSACESSSECPLLSCSFNPSSLSGWGRSDINFA
                     RAELINDFSDPKNIEFTPSLILFYAPKNSPPSKTA"
BASE COUNT         5366 a         3411 c         3507 g         5312 t
ORIGIN      
        1 catcggcaat gccgtttgag atcctttcgt ttccgtcaag atcctcagaa aaacttactg
       61 ccattccgcc tgtctcaaac ggtgcgttcg tccctttgtc aatgcagggt gaaggacttt
      121 tgagtgagaa atctaaatac agcatatctg tgaacaatgg atcagtttca aaatttccgc
      181 cggcatcgac accgagagaa tcgtcccata ctgtatcaaa gaaactgttc tgtatgtcac
      241 tgttggtaat aacaggatct gaagaattgt tgaatatttc atttccctcg ttttcagacg
      301 aattaaaata aaaaatacag ttggtgatca ttggattaga atttatctgg ttatacatcc
      361 ctccgccgta tccgaactga ccggtcgcag aattattcac aaaagtattg gctatgaaat
      421 gtcccgatga accggtgttt gccacagctc ctccgttgta agccgtattt tggtaaaata
      481 gagagctgac cacgatcgga gtacctgtgt ttgtcaacat tgctccaccg tcacttatgc
      541 cggttgctga attgtttttg aaaatgcagt tgacgatctt cgggcttgca tcgctgaaat
      601 tctcaattgc tccgccgccg acacttgcag tattgttctc aaagatcgag tcactgaagt
      661 aagggctgct ctgagagttt gaaaccgctc ctccgttatg tgcctcattg cctgagaaaa
      721 ttcctccggt aaccgttact cctgaatcaa cggtattatt cattgctccg ccgtcatttg
      781 cagaattgtc tgtaaaacgg cagtcaatga aaaagcccct gctttttccc ttgttgaaaa
      841 cagctccgcc ataaccgtca ggtgcagagt tgttcctgaa tacggtgttc tggacataag
      901 gacttgaaat gtcattgctc aacgcaccgc ctcttgcgga attgttttct tcaaagactg
      961 agtcatatat ctgcggatgg cttttgaggt ttgccattgc tccgcctgca ataagagcat
     1021 aatttccttt gaaaatgcaa tttcttatcg tgggatttgc ggtgcttctg ttgtttatgc
     1081 cgccaccctg attcgggtaa agatcgaccc ccggagaaag agaataatcg gcattgccgc
     1141 ccgttatcgt gaaaccgtca agcacagtat cttcataaat attgatgtta agcatgacat
     1201 tgtatgcatc accgctgctc agtcctgcaa tttcatcaat gtcgccactg agtattgttt
     1261 cattctctgc cggatctctt tgatcctttt ctgtttcacc ggctgtaaaa ccacccagaa
     1321 cagcaacctt gtctttcaac gagaaatgga agaaccgctc atatccttca tcgccgggat
     1381 tggaaacggg agtaatttct ttcaccaccc tgtccggttt gtaagttcct tttgcaaccc
     1441 atatctcata accctcttca gccagatcta ttgcatcctg aagttttacg aaagcatctt
     1501 tccatgacag tccggtattt gcaccggttg cgtttaaatt gacataaaac ctgagaggaa
     1561 cgaaagtgtc ctcatcggaa atatcttcgt ctgaatctgt taaaccttca tcggacagat
     1621 catcagattc ctcttcgtct attaaaggat ccgattcgtc ctgaacagtt tcggaatcaa
     1681 cagtatcttc atcgaaaaca agcaaatctt catcagaaat atcatcggaa accggttttt
     1741 tctccttttt gcatgaaacg gaaaagatca aagcgatgag cagcagtaaa agcatctttt
     1801 tgttcatgaa actctccaag aatcagtttc tcaacgattt ttccttttat taaagctgta
     1861 tgccagcatc gagataattg ttataaatcc gaaagttgaa agaataaccc atattctcca
     1921 tgggtgatac gcttcccaga gaatatggtt cgcctcatac tggtcgacac ccataatctg
     1981 ctgaaaatat ggaaaaaagt taaaagtttc gatattcatt ttttcaatgg tttcaggaag
     2041 gagtttgtca ggatttgatc ccttgtattt ttcaacgata tctttcagtg tatcaagagg
     2101 aactcttttt acctcaagca gatactgtct tgtgataata agtttgtttg caaaagcgtc
     2161 gtaaagagga ccggaaacca tatttccaac tatccagccg agcgcaaaag ggatgtttgc
     2221 aagacccatg taaagcgctt ttttatcagc cggtgcgttt actccgatat attcactgaa
     2281 tttcggtgaa caggtcatct ccccgaaact gaagaaaaca atacagatcg ctgtgatggt
     2341 tcctgccata aaaagtccgg cagtgagaaa tgcaaaagtt gtaacgaaaa ttcctgcggt
     2401 tattgcaaca agacggggaa atttcccggt aatgatgctc caggggacca tgaaaagaat
     2461 aatgcacaga gcattgatgt tgattatctg ttcggccttc acacttccat ggtctgtgag
     2521 aaaagcagga agaagttttg cgagatctct gctgtcaatc cactggtcaa tgaaattcgg
     2581 cagaagatcc cacaactgca tgaacataag ccagaacccg ctgaagatca tgagaaatat
     2641 catgaaatcc ttgtctttta aagcggtcaa ggtgttttgc aaggcctgtt ttgctgatct
     2701 tcctgtcttt gccgccggct ccttataaaa gaaaatggtc ggaataaaat tcactgcagt
     2761 tgcaacagcc gcagcataaa aaacaaaatg ccaggtatac ccgtcagtat tatttcccct
     2821 taaagcgctt gccatcaaag gagctatgaa tccgccgata tttactatcc agtagaagat
     2881 gccgaatccg agagaactgt tttcctcatt tactgaagaa gctatggttc cctgtaccgg
     2941 cggcttgaat atagctgttc ccgttccgac ggtgattccc gcaatcatca ttgtccagaa
     3001 ccccgtggcg ttcgccatga gaatgtatcc gaaaatgtta agggaaaaag ctgtatagag
     3061 acttctttta tagccgaaat tgtcggtgaa agaaccgctg aacatgggta tcaagctctg
     3121 acaggccgcc cagacactga aaataagacc tttttcagta aaagtaagac caagcccgtg
     3181 ctggtccgcg gttgcaacca tccagagtgg aagaacagct ctcacagaaa aaaatgcaag
     3241 acgctcaaat acctccatca catttacgat ccagtagttt ttctgaaaac cttttacctg
     3301 agagaaaaaa ctcatttaat gcccctgtat taatgttaaa ttacatccat tatcctctgc
     3361 cggaacagta tcgttctgtt cattatctgt tccgtttgtg tcggtcatgt ccgaattatc
     3421 tgaatcagga atatcaagcg aatgatcatt ggattcgcta tcgtttacag catcaggtat
     3481 ttcaaggtcg gaccggtcac acttataatc aaaagaagtg ttttccagca catcatttac
     3541 accattcctg tctatatcca tcaccagatc agtatctccg gagacaatgt gtctgacaaa
     3601 atagagagca ggagtaatat tttccttgac tgtgtctatg atctgatctt ccggaagcat
     3661 gaaaaaatag tcgctgccca tgtgcggtct caattcaaaa gtgaaagaag gtttcccgtc
     3721 gtgagtgttc atgaaccatt ctgtcatttc tccgaaagtg aaataaaggg atgaattctg
     3781 catgacaata tagacacttc cgcattctga aaaggcgaga taagccattt tttcagcaag
     3841 atccttgtaa tattcatgtt ttgcagcggg ctcttcggtt gaacccggag gatacattat
     3901 gatctgaccg taagaatggt atgttatcag gccgtcaaga gattttattc cgtctgctaa
     3961 gggttcttcg aaatcattta aaataagatt tttcacggcc agagtttccg gctcactgaa
     4021 aggtccttcg ccgtgcacat aatcaatttt tatccaggag gaatcatagt ttctgttaag
     4081 atctacacca attgaatcgt cgggatggac tctcctgttg tatcgccaca ttctcgcttc
     4141 ttcccatgaa aaaagatatc catcagggtt aagcataggg acaacccaga taacagaatt
     4201 ctgaagaata tcagatacaa agggattgga atccatattt tcaacaagga actcaagtat
     4261 tcccatagtc acttcagaag atatccattc tcttgcatga tgaagaccca caaaaagaac
     4321 ctcaggaaga tcttcatcag tcttttcgtt ctttgaaatt cgcacagccc tgatgttccg
     4381 cccttcaata gataatccaa tatcaaaaac ctcagctttc ccgccggacc tttcaacgac
     4441 tctatcaatt cttttgttta ttcctgtaag atcggaatac tcgggaaaga tagttattga
     4501 tctgttcatt tctttgatta cacaattccc gtaaagcagg gctatctcac cgggggagga
     4561 gtctacaaca tattttaaac ccgttccttc tgagggggcg gcacttataa tatcaagaaa
     4621 actgacaaga tattttgatt tctgatcgta ccgagggatg cttatctcat ataactcacc
     4681 gtaaacattt gccaaaaaca gggttatgaa aatcataaaa acaaatcttt tcaatacatc
     4741 atcctccatg aaggaacttt ccggacatta tactttttat aatatggtag tcaaaacttg
     4801 atatgtttca gatgctggta tattttaata gtgttaatag ttgagagaga attatgctgg
     4861 aaaaggataa aacacaatat attgtggaaa agagaaggaa attgatattg tctcttgttc
     4921 ttgtaactgt tttcggatca gttctttata tttcaggaat tattcacaaa caggatgaaa
     4981 ataaagagga gtctgcttca gggggacttt ttgacattgc aaaaaaatta gagaacgagg
     5041 tttctgaatc agcggggaaa aaaatgactt ctgaaaattt gttgaaagca cctgtttccc
     5101 tattgtattc aattgaaaat gataaaataa agctcaagga aacattcagg ataaataaaa
     5161 atgtaattac agatggcgtt tttacttttg acacacacgg agatactatt tactcaaccg
     5221 ggtttatttc aaaagaaaga catgttaact gtaaaggtgt catgaaaacg ggaaatgata
     5281 gatttttcat atacttctgc acagagaaaa cgccatagct ctataataat aaataagtta
     5341 aggaataatt gacataaaaa cttgaaaatc gataaaaaac aggttaaata ccttgagaat
     5401 tattttttgc tgcagtgttc tgtaaaggag gaagaagttg agaaggattt tagttttaag
     5461 tttgtttatg atgttcatat tccctttgta cgcagataaa gttcttttta tgagaccggt
     5521 tacaaaggag attgaaaaat ctgatgcgat caaaatagtc cgtgatcttg agtcggtttt
     5581 aaattcaaga tttaactcag taagttataa agatgttccg aaagggaaat tagctaaact
     5641 tgctaaatgc ggaatgaaaa cagagtgttg gtatgataac gcgagtgatg atgatttcca
     5701 gtatgctctt ctttcgctta ttagtatgaa tgaagatgaa gagataacca tcagattcgt
     5761 gttcattaac atcgaatatg aagagatact tgacgattca tcaaagacat atgactacat
     5821 cgactcagtc aatgaaaaag aaatatacag acagctcaga aaaacgacat cgtctgtttt
     5881 tgatgaaata aaatccggaa caggcagcag aaaatctgca aagagggaca gcagaaaaag
     5941 ccgtagctac agtagagatt ctgattacga agaagacgaa gataacgaga tatccgccag
     6001 aaaagaaaaa gctgctcaag ccgaagagga aagaagaaga agagaagagg agcgcagggc
     6061 caaacttgaa gaagagagaa ggcagagaga gcttgagatc gaaaaaagaa gagaagagga
     6121 agatagaaga agaaaagaac ttgaaagaaa gaaaagaata gaagaagaaa gaaaaagaag
     6181 agaagaggaa gctgaaagag agcgtcttga aaaagaaaga gaacgcaggg aagaggaaaa
     6241 aaggagagag caggaatcca aaaaacagac acttatggat aatgccgaaa agcttgcaag
     6301 agcaagagag ctcgttcttg aaatgtgttc tcagggaaaa tataatgccg ctattaaagc
     6361 aatcgttaaa gtaagtgaga taaaatgtga gtgtgaagag gatgcaaaag ttcttgcact
     6421 taaaacacag cttcttaact ttaacaaaat gagagataag atccttgaag gtgtcaagct
     6481 tcttgattac tctctgatcc ttgacaatct tgaagctgcc aaagctcttg atgaatatat
     6541 tgttcccgga ggaactgaat tctcaaaaag aattgataag atagaagctg tcggttatat
     6601 ggcaaaaggc cgtgatatgg agaaaaaaga caactatatt cttgcaaacg aagcttttga
     6661 aaagtgtgcg gaccttgatt ctgaaaaaac agaatgtcgt gaatggcttg attcaaaaga
     6721 taaacttgta aaaaggatat atgacaaagc ttctgtaatg aagagcttta acccgactaa
     6781 agcaaaagag ctgctgagat caattcttaa acttgttaca gcagataacg aacattataa
     6841 gaaagccgaa gcagatcttc agaaaatgga atactgatca ttcccggatc attaaaattg
     6901 tctgtcatac aacttacccg catattattt ctactggttc tcttgttttc acataacggt
     6961 tgctctgaat caagggattc attacaattt tgcagccgta gtgattgcag ttattcaatt
     7021 gttttaccag gaatgatctc aatttctgaa ggagaagatc cggaaaaatt ttttctttta
     7081 actgaaaaag gattgaaaaa aataaatttt gacattaagg aaggttttcc ttttgcatat
     7141 gatataaaga aaaatatgtg tggcttaaag aaatcggata tggatggttt tttaataaag
     7201 atatttgatg tcaaagataa taaaaatatt gttgagacat ctcttccgga aacagtatca
     7261 tcaatctttt caggttgttt tctgaaaaac ggttcacttg cgatgctgct gcttgaagaa
     7321 aatgagaacc tcagcagaag atattttttg tcattgtcag aaggattgga tattaatagc
     7381 tggagaagtt acttaataag agaggaaacc tcatcagatc tgataggaaa gatcctttct
     7441 ttgggtagtt catttgaaag accggtttcg atacagtgtt cagatgacag gattttcatt
     7501 cataccctta caacctacag tgacatgata caggccagta tttacagatt tgatgttgaa
     7561 aaaaaagctc ttgtctttga aactggatat catcctttaa atgtaaaaga cggaactgtg
     7621 tctattttct ttaatgagaa aaaaaacact ggttatgttc atcagaaaag agaacttgtg
     7681 attttgaaag gatctgattt tcccgtaaaa acaaagttcg atgaaccggg agaagttttc
     7741 ttttcacctg cgtctgatgg agagggtctt ctaatatatt ttattcctga aactaaagat
     7801 catgttcctg caaaagcaag aataatgcaa tttgattgat cagaacagtt gaacatatgc
     7861 aatgacaaag accgaaagtg ccgcagttgt ccagatcgtt ctcttgtaga tcactcttac
     7921 aaaatctttt ctcagataaa aatagtgatg aagaacaaca aagatcgaaa gaaggggaat
     7981 tattgatgcg atcttgtatt gagtaaactg aaaaagtatt attgaagata ttttgaaaat
     8041 cagcgacatt gttattgccg aaggaattcc cttcgttgaa gaaatcgttt ttcttcctgc
     8101 cgcagtgtct ccttcaatat cagtcagatc tttcagtgat gttgttccta ttgcgtggag
     8161 cataacactt gacgaaaaaa gaaaaagagc cgttccgcct gttatgaaaa gaggatgagt
     8221 cactccccag ccggtaatta ccggggcaag gagagctctt ttcttgatta ctaaaggcgg
     8281 cattgaatag ctgaatccaa gaagaattat taaaaacgca aggaacatct caactccttt
     8341 gaagatcaaa gatattccgc tgactgatat aacggtgaga aataaaagcc agatcaaagc
     8401 ttcatttttt gtaatcagtc ctgatggaat gggtctcccc ggtttgttga tggaatcttc
     8461 agttacatct gttattgaat tgagcgtata aacgaaatta tatggaaaaa gtgacaggaa
     8521 aagagcttca aatatcacaa aaactgaaaa acctgtggtg aaaactctgc cggcaaatgt
     8581 tccaaggaaa acgatcacag gaacatcagg cctgtaaagt ttaaaaaaac cgactgtttt
     8641 caggatcact ttgttcatcg ctttcctcaa gcttaattca gaaaacgatg ttacataatt
     8701 aaagatttaa tgcaagacct tgactaaacc tgtgcaatgt ggatatttag tatacgattt
     8761 tcggggagtt tgaggaatgg gtgttattgc tcgggttaca ggtcacggaa gacattacta
     8821 tacagtttta tctgaaaaag gtgagtttaa ggcgaaactt gccggttcgc tcagatataa
     8881 ttctgttttc aaatcagatc ttccggctgt gggcgacatg gttgaaattt cattaagagg
     8941 ggataaggca tatattcttt ccgtttttga aaggaaaagc tgtttttcaa gaaggagttc
     9001 gggaaatgtt attgatgagc aggttcttgc tgcaaatctg gatattcttt ttgttgtcat
     9061 gtcccttgat gaaaattaca atctgaaacg gcttcagcga tatatttcgg cagcaagggc
     9121 agggaatata aaaccggttg ttctgcttag taaattggat ctttgcagtg atcctgatca
     9181 tttgacacag caggtaagaa atatggctga aggagttgaa gtttttgcgg tatcatcagc
     9241 aaatagagag actttggaaa ttattaatat gtttcttgtt acaggaacga ttgctgcttt
     9301 tacgggctca tcaggggtcg gcaaatccac gctgataaac atgcttcttg gcgaagaaag
     9361 acagaaaacc gctgaaataa gcagcgctgt taaaaaagga agacacacca cttcatcgcg
     9421 tgatatgata tttctgaata acggagcgat agttatagat actcccggaa ttcgtgaaat
     9481 tcagatgtgg gaaactgaag tccctgaagg atttgaaaag attcttgaac ttgccgccgg
     9541 ctgccgtttt tctgattgca tacacgatac agagcccgac tgtgctgtaa aaaaagcagt
     9601 gtctgaagga ttgctgtctt ccggaattct tgaaatgtgg agaattcagc aggttgaaat
     9661 ggatgagctg caacagaaaa aagaggagtc ggtaaagatc cttgagagtc gaaaagctaa
     9721 aaagaggagg agctgatgag ttcaatgaga aaaagttttg cggcaacgat cctgttcttc
     9781 tttttgtgtg cgtgtgctac atttgaagaa aagcccgata ccgagaaaat ggctgaactt
     9841 gaaagtatga agataaaaaa agtcgagccg gagaataaag aagacccgat agtggatcat
     9901 ggaagacatg ttctgataaa tggagataca tatatcgggg gcttcaggaa gggaaaaatg
     9961 gaaggagaag gtgaatatct ttacagaaac gggaacagat acaagggaat tttcaaaaat
    10021 gatgaaatgg atggagcggg aacattcacc tataaagacg gaacggttta taaaggggat
    10081 ttcagtgaag ggaaaaaaca tggtttcggt gaaataattt tcaggaacgg aaactggtat
    10141 aaaggaactt tttattatga cattattgac ggagaaggga catatttcta taaaaacatt
    10201 ggtgaatata aaggaacttt catcaacgga aagagagaag gaaaaggtgt gatgaaattc
    10261 aataacggtt ctgtttatga aggcgactat ttaaaagacc gccgcactgg aaaagggaag
    10321 cttacacttg ctgacggaac agtttatgaa ggtgactttt ttaatggtgc aaaacaggga
    10381 aaggggacaa ttgttttttc aaatggagac gaatacacag gtgattttaa aaaagacctt
    10441 ttccacggac aggggacata taaatttgca accgacggca gagtgtacac aggtgaagct
    10501 gttctgggaa aacttaacgg gccgggcgtt atcacatggc ccaacggtga taagtatgat
    10561 ggagaaataa aggacggagc tcctcacgga atcggcactc tctataaatc cgacggagaa
    10621 gaagtcacag ccgaatgggc acagggtaag atcatttttt caaagtgaga gttattctgt
    10681 caaaccgcca tcagttattg tccagttaaa atcgcttatt atcttagctc ttgatgtttc
    10741 accagaggaa ctgtatttgg agttcccacc atgaaattca acactgtttt gaaggataag
    10801 tttgctccag ccattaagca gagcatcgta gttcgcagtt gaaagagtgg tgttgtagaa
    10861 catttcctgc atatatttta cattcgaaac attccagccg gaaatatcct gatcaaaaga
    10921 ataagcatga gaaaacattt tgtacatatt tactacattt gaaacattcc aagttgttaa
    10981 gtcaccttta aatgagattg caccattgaa aagaaaactc atgtcggtta catttgacac
    11041 atcccagccg gaaatatcct gattaaaaga gacagccccc ttaaacatgc tgttcatgtc
    11101 tgtaactttg gaaacattcc agccggaaat attctgatta aaagattcat gaccgttaaa
    11161 cattccgctc atattcgtta ctttggaaac attccagccg gttatgtcat gatcaaacat
    11221 ttttgctccg ctgaaaagat gtcccatgtc ggctacattt gatacatccc agccggaaat
    11281 atcctgatta aaagagacgg cccccttaaa catgctgttc atatctgtaa ctttggaaac
    11341 attccagccg gaaatgtcct gattgaaaat tctggcgtta ttaaacatat cgcccatgtt
    11401 tttaataccg gaaacattca aatttttgaa aaaatcagtt ccgacaagta tgtaacagtc
    11461 ttgaaataca cctctcagac tctctgtttc acttaaatcc ggcaaatcta ctgctttaat
    11521 ttcaagattg tagcaaccgg aaaattgaaa ttctgtttta ccgaaggaaa atgttcccca
    11581 ttgaattatt tctgtgattt ttccggaatc actgttttca catttttcct ctgtttcatt
    11641 gaaattacaa aactgccagc cggagatatc accttcaatg acaatggtgt aagtcccttt
    11701 tttgttataa cggtgtaata acgcagggtc gttccatttt gtgattttat tttctgttcc
    11761 gtctccccag taaacagtaa aattgtatgt gccgttatca accagaggaa gttttatact
    11821 gttttcatct gaatatccgt cataggttgt tgtccatttt gaaataaatg aatctggaag
    11881 agcacacttg ccgccgatac agcttctttc ttccggacat cctttgtctg aaaaacaagt
    11941 cctgttgttt gtgtcgttca cacctgttac acataataca tcggcatttt ctgtcttgac
    12001 tgttccttcg atggttcccc tttcaaaatc aacagtcaat ccataaaaat catagtacat
    12061 ggaatccgtt gtagatgacc agagtttttg agaagttatt cctgacatat cagatgcagg
    12121 atcttgtttt gttatgttaa caagagtttt aagctcactt atgttaggca gacgccagtt
    12181 agcttttcct ccgtagttta aactctcgca tgtcatcaat gccgttttcc agtttttatt
    12241 ggtaaactga gtgtctgtcc atataagatt tactgttgaa tcgactataa tgcggtcgtt
    12301 tctgattgtt ttatttgaaa aattattatt gagtgaaagt ttgggacctc taacacatcg
    12361 tgcataatga ctgctgtttt tgtatcgctc tctagtacct ccataaataa aatcgactgt
    12421 ccacccgatt tcattgtcaa gtggatcttc cgaagatgtc cagaaagaat ctgcaggtgt
    12481 aaagggaaaa acatcggtgt taattgccgg agactcttct gctccaagat caatgattgt
    12541 ccaaagctct tcataggttg ggagtcgcca gtcatcgaaa ccaccgtaat tcaggtcttt
    12601 acagttttca actgcctgat cccattcata ttttcttaaa taaaacattt gctgccactc
    12661 aaggtgtgtg ttgttgtcaa tggcaacagg ccccgaaccg ttatctttta tcgaaaaact
    12721 cttacttaca cataaattat ccgtgctata ttgtccatcc tgtccgtaat aattgctgtt
    12781 ttcatttgga cattcaattt ccgtgttgtc atcatagcac ttcgtctgac ccgtgcatac
    12841 aactccttca atttcagcag tatattcatc atcagcttca atatcggagt caggaagaaa
    12901 aggatcgtca gttaaatcat gttcaagatc tgtattatcg gttgtatctt cttcacttgc
    12961 cgtttcaggt ctttcaaact tttcaagact gcacgaaata aaagtaaaca gaattgccat
    13021 caacaaaagt cttttcataa ttatctcccg acaacaatct cttaagcagc tgattgtatt
    13081 gaaaataaaa agatgttgca agtttaaaaa ttaatatggg aattttggtt gtttatattt
    13141 catatctcac cttttatgcc aagaacaaat gttctcggac ttctcggtcc gtagatatat
    13201 ccggcatccc ttccggctcc ttcatcaaaa tcatcctgga atgagttgag gacatttttt
    13261 actgtgaaat aaggtttcac gctgtagctg tcggctttaa atttatatcc gattccgatg
    13321 ttccagtcag caaaccagtc tgaagttgtg agttcaaaat cgctgccgtt aaaatcttct
    13381 tcgtgcttta ttttcatagg gcctgtaaga ttcagtgcag ttgtaaactc aagccctttc
    13441 cacggggtca gcgcagcagc tatgtaaccg taaaaattcg gggttttaag cactcttttt
    13501 tcatttattc cgacacctgc gaggtcttca tcgtttttac tattttcagc tttttcaaat
    13561 gtccagccga tatcaagagt ccacaatttt ttataagcca tttcaatatc aagctctatt
    13621 cccatggaag ttgtagttcc tgagttataa cggatctttt tatttatccc ttcttcataa
    13681 ttagaatcgc tttcttcaat gtctttagtg ttgaaagcgt catatatctg acttaaataa
    13741 ccgttgattg aaggtttgat cgaaaaaccg ttcttagtga atttatattc aaactgttgc
    13801 gaaatattcc aggatttttc ggcattaaga tcgtctgaat ttactgtcgg tgagggctct
    13861 ccctgaacaa cagtaatatg aaagtcctca tcaaatatct ggggtgccct gaatccggtt
    13921 gaaaaagagg tccgtgattt aaaatccctg tgagagaaca ttagtgtcgc tcttggcgaa
    13981 aaaaccggaa catcgagttc gctgtgtttg tcgattctaa cccccataat tatctcaacc
    14041 cattttttcg gcatccagtc aatctggatc aagccaccga tattgctgta tgtttcatca
    14101 gtcgaagcta agccgtcagc cgtataatcg tcaagatctt cgtgcgtata ttgaaatccg
    14161 ccagtaaaca tcatcacgcc gagaaaattt gcaagctgat ttatcgaggc atccagaaca
    14221 tgcatgggat tttttgtttt tccatacatg tctccaaact gatcgtctcc gccgtaatat
    14281 gaacttcttt cggtatatgc aaacccgtaa gacatttcat aatcagtttt ggttgaaaga
    14341 atttcgtgtt tccattttac ttctcctccc catctgtctg tgtcagtgct ttcagcaact
    14401 tcggatttgt gttttgcccg caaaaaatga tctccgcctc ttcgtgaatc cctcataatg
    14461 ctgaactttc cctgcaattc accccctttg aacgggttta gaaatgcatt tgagccgcca
    14521 aaagactgcc ttgtttctcc aagttcggaa aatccgtcat cattttcatc ccacggctgt
    14581 ctggcgagcc ctccgccata gacaaaaaga gccgcctttt tatcttttgt cacaagtccg
    14641 gcgtctccac ccagccgcaa tccttttgaa agtccgtcaa cgccgctttc acttggaatg
    14701 aactgtgttt ccaaagttat attgccaaaa ttttcagagg gcttctttgt gatgacattt
    14761 ataactccgg caacggcact tccgccgtaa agggaacttc caccgccttt tacgatctca
    14821 actctttcga tcatctgaac aggaatgtgt tcaagtccgt aaactcctgc aagtgaagag
    14881 taaactcctt tgccgtcaat gagtatctgg ctgtagtgac cgtcaagccc gttaagccga
    14941 acctgattaa atccgcagtt ttgacagttg ttttcaaccc ttacgcctgt tgtgttatca
    15001 atcgcatcag ctaaattagc cgcattcttt ttctccattt ccgccttgcc gacaaccgat
    15061 gtttttacag gagcttcaga aacaagtttt tcagtttttg ttccagttac aacgatctcc
    15121 tcttcagttg aatcttcaac agtttctttt tcttcttctt ttttcttttc ttcaatctca
    15181 gtctcttcat cctcaacaat ttcaacatcg tgtttttcat ccgcgattgt taaaaagggt
    15241 gtctgaaaca tagtgataag tataagcatc tgtattttca tcaatttctc cgtttttaag
    15301 aattagttaa ttcatatcgt ataataatat tccgttttat ctttaaatga cctatttcga
    15361 ctggtttttt tatcctgccg ctgcttttct taacactttc aaaagctgct tcatcaagaa
    15421 tttcctgacc tgaagacttt gtaattttta tttctttgaa agttccgtca gtatcgatca
    15481 gaaatgagat ctttacaact ccttcaatgt tgtacctttg tgcaaacgag ggataaattt
    15541 tgttccttgc aatttcagtg ttcacttttt ttgtgtattc agaaaggatc ttttttgtgt
    15601 caaccttttt ttctgccggt ttttgcggtt cttccggttt tccaattcct gcttcatatc
    15661 tgccggcttt tgcatcaacc atttccgcca ttctcagccc atcggaagga gaggcaacgt
    15721 ctgtgatctc ttctcggagt tcctccggtt tttcaatgac atgctctttt ttaatttttg
    15781 gtttcggttt aggtttaggc ggtggtggcg gaggaggtgg cggtgcagga ggcggttctt
    15841 caaagttcag cagaattttt gtggttgtga caatagagct ttccttgaac agatcagtct
    15901 ttgaagctat taaaacaccg tgaaaaataa ttgaaagaat tacggcaaga gcaaatattc
    15961 tgtcactgtt cattgatttc attctccgcc tttttttcag cggcaattgc aagattttca
    16021 aatccgagct ttcttgccgt atccattaca aaaatgaaaa gtccgaaatg tgcggtttca
    16081 tcagctttaa gtgttattac atcatgcttt tcatttgtag ccagctcttt ttccttttta
    16141 agttctttca gtacattatc tttttcaaca actgttccgt tgaaagtaat gacattgtct
    16201 ttagttactg ttatctcaag tgctttcgga ggtctttttt cagcagtagc agccgatgga
    16261 agcttgatgt tcagcccttt ctgtgaaatt acagccgtta tcagaaagaa aatgagaaga
    16321 ttgaagatta tgtctatcat cggagtaagg tttacttccc ggttagattt tctttttaag
    16381 ttctgactga atttaatcat ctgaaagccc ccgaatttct catcaaagag acaattttct
    16441 gtgctttctg catcatccgg tgggatattt tatcaacttt tctttcaaga tagttatgtg
    16501 caaaaagaat cggaatcgcc acagcaagtc ctgccgctgt tgtaaggagt gcttcccata
    16561 ttcctcctgc gagaatgctt atgtcgctca tgtctcctgc cgttgcaaca cgggaaaata
    16621 ctttgatcat accgacaacc gttccaagaa gtcccattaa cggcgtaaca gttccaagca
    16681 tggaaagaag atcaagccgc cggtttagat taaaaagaat gtcgtctccg gcagttgacg
    16741 ccgccttttc acactgctct tcagactgtt ctttgaggat cgcggtaaga aaactactca
    16801 taatcccttt ttcagattca atcattgaca gagtttcttt aactcccttt tgttcaattg
    16861 agttgaatat ttctcttaaa ttttctttgt tcagcatttt tatagttgaa aaaacgaaca
    16921 tcctttcgac aaacacagca agggcgacaa cagaagacag tatgatggga tacatcataa
    16981 atcccccttt ttcaataaac tccatcataa tacaactcct taaaatcatt aaactttaaa
    17041 attttacggg aattttcagg cagtttttga aggaggtgag tttttaggag cgtaaaaaag
    17101 aattaaagat ggtgtgaact caatattttt aggatcggaa aaatcattga tcagttctgc
    17161 acgggcaaaa tttatatcgc ttctgcccca gcccgaaaga gaactcggat taaaactgca
    17221 tgacaaaaga gggcattcag aacttgattc acatgctgaa aaaaaggaat atgggtcaaa
    17281 aatcccttct tctgagttgt ggtgatagtt acttgcagct ttccagttga aataaacatc
    17341 aacaaacgcc tgatcttctt gtgaaaattt gtgagtgata ttggaaaagt ggagaatcgc
    17401 aagatatccg tcaagaacat aaaatatgct taaaatgaat aatgtaacaa aacggaccct
    17461 tttcaacatt gagacacccg tcaacgaatt tcacagccac aacatagtta gccatggcta
    17521 atttgtcaag aggttttatt gcaattgaag ttcccccaaa aaaaattcag cagatttcta
    17581 actgaagagg aggagc
//
LOCUS       DAOOYB010000030        17163 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_3231_length_17163_cov_14.135083, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000030 DAOOYB010000000
VERSION     DAOOYB010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 17163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17163
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3231_length_17163_cov_14.135083"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /locus_tag="PL056_03000"
     CDS             complement(<1..742)
                     /locus_tag="PL056_03000"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPM46017.1"
                     /translation="MKVSIITTVKDDAKGFLQTMQSVKMQDYSNIEYIVVDASENERL
                     KDKCREHIEKIDVFIEGADSSPYEGMNRGIDAAAGEIIALLHAGDVYCSNSIVSQVVE
                     KITENDIKLVYGDIVYHSIEDVEKTVRVWKSAQIGSDSHENGIFPPHTSLFIKKELYD
                     RVGRYDLQYEIAADSDFMFRLFSQNPKSLYLHKNILSMRVGGRSNRSLFAILRSNIEF
                     CRMLRKHGVSHPFLKTLKKIFSKVRQLFS"
     gene            complement(739..1671)
                     /locus_tag="PL056_03005"
     CDS             complement(739..1671)
                     /locus_tag="PL056_03005"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HPM46018.1"
                     /translation="MTVFPEITLRKEFRGLESDPYLKAIGFIKGGQPSFKGGYVATEA
                     ANIIKYSHLRKITSKMKESGFSKFMPVKGMMLLNTLFSDFQGIRQMADIDILVHPDEF
                     GKVAEFVNNNQEFNYKSNYPVFLRKYFGEDISFYFDSTLVEIHSRITLVKFPGLIEEI
                     FEKSEEKSNPDGEIFIVPPLEYAAVIMLLHDYSRGDFADLTFKRLLEFYIVAGNCDLQ
                     KLKSIAEKYGLEQMLNCHLFLIWTMLDNTFFDRYDFKIIEEFGLITKHERLNCFKVEK
                     PYNLQKALYGKRWKYLKMRNIAAGVFKFAIGKKD"
     gene            1801..2331
                     /locus_tag="PL056_03010"
     CDS             1801..2331
                     /locus_tag="PL056_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019225962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HPM46019.1"
                     /translation="MCNFKTIVPEEAKLNPFLTLKNEWGLLTAGNFEDFNMMTIAWGY
                     FGIMWNKPVFGAVVRPNRYTYEFMEKNSTFTVSFFPPEFRAELEFCGSKSGRDVNKVF
                     ETSFEPVDNNGSVYFSQADIVLVCTKLCFQDFDPENFVDPEIIKHYPEKSFHRLYIGE
                     ITQTLQKEKHKNKVSH"
     gene            2328..3023
                     /locus_tag="PL056_03015"
     CDS             2328..3023
                     /locus_tag="PL056_03015"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPM46020.1"
                     /translation="MKGFAVLVSNCAGRQELKAEHGLSLFIRYGENDYLFDTGGSDLF
                     IENSQFMNLGIENIKCAVLSHFHLDHIGGIPFLSRHFSMISGICDVYHPAFSEILKFP
                     ALRSNTVRESQKIDDECALVLSNAVKDDENFNEISMVAGNTLFTGCGHSGILNILKEA
                     QNFSTVTTIAGGFHNFDMQKDKMKKTASDLFLLGIKKVILLHCSSVNSIRYFEDAGIE
                     CIFGSVGNSFNIE"
     gene            3023..3946
                     /locus_tag="PL056_03020"
     CDS             3023..3946
                     /locus_tag="PL056_03020"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HPM46021.1"
                     /translation="MDNNQISKVLEHFNIEFENTSSFEIYGAPSRSADRAVVTDKNNE
                     KFIIEKHHFWNMSKKVKIAENINLLYNNGFEECSPYIYSVQGPPVIEFENRCWMVQKF
                     IHHDILQRPDYLDDGLKGTAAAETLLKMYEISSNLKMNDTIDSFSLAEHTLKTVRKIR
                     NIHPEILSKISDVINIIEHSFMPLIEEMPLVFCHGDYHPLNILWDGNNLKKVIDWEFS
                     GYRPMLTDIANIIGCAGFENISAFDRNFVRKFLQVIDKYHYFKRYDFNAIIIFVIGFR
                     FSGWLNEWIKDKDGAMVQRETEYLNFLREMI"
     gene            3943..4371
                     /locus_tag="PL056_03025"
     CDS             3943..4371
                     /locus_tag="PL056_03025"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPM46022.1"
                     /translation="MIRPLRSSDIPAIEKIYSFANESIGLPVNKDHFRKDAKKYVSET
                     IYKCENMVYELNGVPAGIISVSSDYIEGIFVDPRFFKKGIGSALIKHFLNKKEYLQLQ
                     VYELNHNALNFYRSHGFVITGGGICRITGLPYFEMVYERK"
     gene            complement(4375..5358)
                     /locus_tag="PL056_03030"
     CDS             complement(4375..5358)
                     /locus_tag="PL056_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46023.1"
                     /translation="MPDKITAFLLVLLFFWGCSSEMKVDETKWSDEDIPFEITDVTDH
                     DTIENDDEISEENQDLENYDEDFTDVETEPDADSDETTDEDASAPNCANATCSDLGFC
                     FVNGGVPECFCPSGYHSEFPDCIANNPASLCSGVECSGNGSCVAVEVFEKPVCTCEEG
                     YLPIGLACIQNVSGDPCDYVDCGPNSECGFDYTAITCECKTGYLADESGWGCIEDKPD
                     PCEKVDCGEHGKCETEKGRAVCKCDEGYFYNGKTCVSVEEINCDYIDCSGHGECVEAK
                     GIFYCRCDEGYYDSGYECLPETAPECVNDFDCYYKCGIFLGECISSKCKCL"
     gene            complement(5413..5892)
                     /locus_tag="PL056_03035"
     CDS             complement(5413..5892)
                     /locus_tag="PL056_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46024.1"
                     /translation="MSINKIQLVIITFLMISCSGRVESEFGNRCRVEDFKYERKQFSS
                     DITGGIRFQLDENALKNSGKVVTGSLLSLSQGTSDLTLAGGMGENCTDCILMFVRGEV
                     AGNSGYRAVSGKVNVEILNHDTKGRVSFAKGHFSRMVFESIDGSECVVTARLDFVFY"
     gene            complement(5882..6943)
                     /locus_tag="PL056_03040"
     CDS             complement(5882..6943)
                     /locus_tag="PL056_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46025.1"
                     /translation="MKTEGFIKAGSFRKFKYILLLLFFVFFDVSAYEANASVTLGGGW
                     KSDVDPSFSFRSASDIFNRSATRSSATMLFSFEAGVQSEKESGFAFDLFLNGLISLQS
                     VSNSVLDSGIEGGYIQFVNKNNIIAFIAGINNSSCDYRSMKSLYVDPYIAFSYLYAPD
                     EIYAIFLRTGLSYYFSTSSLVEYLTGPSLFLEGGARFNFNENAALDVFAGSSATFFKD
                     QFISYNRYEDVYYGELSIEGRYFSLYSGMNLFWEISSFSFPVSLKYIFSRSFDYDTHK
                     IVYWSDVSIPSTIYEKVRIDSTIEFSAKISYDINDNFSILAGYFLHKTFSNVGDDFGD
                     YADYSRLAHTVSVEVSYEY"
     gene            complement(6921..7445)
                     /locus_tag="PL056_03045"
     CDS             complement(6921..7445)
                     /locus_tag="PL056_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46026.1"
                     /translation="MKIIAFTAMLLFLSPLAAKGEGAEIRSRVRFEKGLSENAISGWE
                     KEFFKISEDVPEAADEIADIEEIPDIDTVKKIKKVRHQKKRTVIRSQNKIENTSPQEI
                     AGTQQDAVPQTVEKTEKIPVVDQHMTEMEVESLEEVPDNLADDVDRSERMRKMKELLK
                     KRKGKGRIEDRRVY"
     gene            complement(7442..8137)
                     /locus_tag="PL056_03050"
     CDS             complement(7442..8137)
                     /locus_tag="PL056_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46027.1"
                     /translation="MDCRDVVKAIDEQIELNESSFLEHISSCENCSKYYASAQKLRAL
                     FSKYDKTLDDTFGDDFDNALFIKLQKADISADRKKFRPSIIQIVSAFLFTVIFSVGIF
                     YFSGTGNDGSEFISSTETELNEPVRIVLEYESDQDIQGVEVSFILDKGVRFYSQNEKF
                     RNLDSFIWKGDLKKGKNEIPFVVSAVEEGSWKIVTEARFEGMSHRHLITFRTDGSKVA
                     VVLYKLEKLSDNI"
     gene            complement(8137..8748)
                     /locus_tag="PL056_03055"
     CDS             complement(8137..8748)
                     /locus_tag="PL056_03055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPM46028.1"
                     /translation="MKNSEKELIKLATQGDKRAFCDLVSMHRAKLRSFSVNIACGNAT
                     LADDILQEALVKAFISMKNFALRGSFTTWLWRIIKNEFINYLRDPSNKLDTFSISTDE
                     KDIEIPDPENSESEMIKSDVREEVHRLISLLPLKLRTAVILVDIQEMCYEEAAEILEI
                     SLTALKSRVFKGRKKLVEIVNGNADTRQLSADRFRSIDDDEEE"
     gene            complement(8750..10414)
                     /locus_tag="PL056_03060"
     CDS             complement(8750..10414)
                     /locus_tag="PL056_03060"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HPM46029.1"
                     /translation="MLTDYEIASSVIQQPIEKIAEKMGLKSEHVIPWGRNVGKICLKA
                     KDLHEPKAKMVLVTAMTPTPAGEGKTTLSIGLSEALNKIGKRSIVTLREPSLGPVFGV
                     KGGAAGGGFSQVIPMDSINLHFTGDIHAVTSAHNLLTALLDNDYSRVEQKCLLPREIV
                     WNRVLDMNDRALRNMVIGLGKEAGMVRESRFDITASSEIMAILGLSRDMADLKKRLSD
                     IIIAENNDGVPLRAGGLNASGALAILLKDAIMPNLVQTLEGNPALIHTGPFANIAHGT
                     NSVIATDIARRYADMVVIEAGFGSDLGAEKFFNLVSRSEGMVPPDVVVIVATVRALKF
                     HGGVKIKSLSEPDVTAVEKGFANLKKHIENMRSFNRPVVVALNRFGSDTTQEIEIIKD
                     LVKEAGSQCFEANVWAEGGKGGVELAKAVYELSQQDHGEVNYTYSFKDEPVEKIRKIA
                     KNIYGAKDIHVPRKVENKIEQRKKWGISEVPVCMAKTQYSFSDNPDLKGSPQNFILEI
                     ADIKYAAGAGFMVVYTGEIMTMPGLPAKPAAALMDIDENGKITGLF"
     gene            complement(10398..12071)
                     /locus_tag="PL056_03065"
     CDS             complement(10398..12071)
                     /locus_tag="PL056_03065"
                     /inference="COORDINATES: protein motif:HMM:NF015515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HPM46030.1"
                     /translation="MRSLKPEFIYCAVFILICFLIIFQGGQTLSIIPPVVAIGLAFLT
                     KQVIISLFCGVWSGAVIVLINNGNSLTYSFFSGFLKTIDNYAVKGLNEESHIMIVLFS
                     LMIGGMVGIITASGGMHGIVAALSKRTDTPSKSLILTWAAGLFIFFDDYANTLIVGNS
                     MRPLTDSHKISREKLSFIVDSTTAPVASVALLSTWIGYEVGLIGDALKASGIESDPYT
                     VFIYSLPYRFYAILLLLFIPVFIWMKRDFGPMLKAEKKAADGYADEENAPPEDFSSFK
                     EIDPSKCRWYTAALPITTLIVVTISGIIVSGYSAFEGGSPTLKDIIASADSFRVLIWG
                     SLIGSIVAAVSMILNRTGSVSTAVSSWVEGVKSMVTAMIILVLAWALSAVVTELKTAE
                     YISFLLSGTLPVWSLPALVFVTASVASFATGTSWGTMALLFPTALPLAVTMAQNASIN
                     LETFIFSVTGAILTGAIFGDHCSPISDTTIMSSMSCRVSHIEHVRTQLPYAFAIGLVS
                     LFFGYLPAGFSVNPFILIIVQLAVIVLVFRYFGKKANNGDKGGLNVDRL"
     gene            complement(12068..12451)
                     /locus_tag="PL056_03070"
     CDS             complement(12068..12451)
                     /locus_tag="PL056_03070"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HPM46031.1"
                     /translation="MSKRKMSRVNFRVDALINYNDMKKVCSVRDLSLSGIYLATDLDL
                     ALGKSVNLTVKIISDSTVGEIELTGIVVRKDHDGLALEFSDLPIDSYLFLRNVVVYNS
                     GDPEAIDQEYRRHLASRKMKTPGDL"
     gene            complement(12470..13255)
                     /locus_tag="PL056_03075"
     CDS             complement(12470..13255)
                     /locus_tag="PL056_03075"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HPM46032.1"
                     /translation="MKLKLILFFAAASFFFSIFSCASSEAKAEKAKSESDPEIGEASM
                     KGGERVAIRGYTADKEMNKKMKQLDLEIIDCYKQFTPIGNASEMTIKYNIIIDESGFV
                     EKASYKRVASSFNKIAECIAKDMKELPFRPGELRELDYTLVFKPLKQKKKIDPDDKRS
                     RMILSLSEMKKFRTCYEEAAAVTPGIGGNFTIKFIVTEEGKAEDMKITGNTFPDPKVP
                     LCVVKKLSETIFPEGESEDVVEVNFKYVSANPPKMRKSMDIDM"
     gene            13396..13974
                     /locus_tag="PL056_03080"
     CDS             13396..13974
                     /locus_tag="PL056_03080"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004438322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HPM46033.1"
                     /translation="MKNLILASGSPRRLELLSYFGFDLKVIKPDTDETVDPALTPQEV
                     VISLALQKGNAVAHIVGTKTPLLSADTIVVIDDEIINKPADKDDAINMISKLSGRTHS
                     VFTGVAIFFNDTVDTFFVKSDVTFKKLSMKEISDYCDTDEPYDKAGAYAVQGIGAFMV
                     KSINGSYSNVIGLPVSEVIEHFQKLNIINGLK"
     gene            14115..15251
                     /locus_tag="PL056_03085"
     CDS             14115..15251
                     /locus_tag="PL056_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008991542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM46034.1"
                     /translation="MIGRYLIEEIRKRMFKEKAIVIMGARQVGKSTLVEMVLENFRND
                     HRILKLNGDDSDTRTIFENPTSTKIRNIISGHTIVYIDEAQRIPEIGLVLKIMVDNFN
                     DLQVIATGSSSFELSGKINEPLTGRKFEYTLFPLSFEEMVRHTNYLEEKRMFEQRLIF
                     GSYPDIVNNNNDAGDLLKWLAGSYLYKDIMALENIQKPVLLEKILKALALQVGSEVSY
                     NEIAQLVGADNATVEKYIDLLERAFIVFKLPALTGNVRNEIKKGKKIYFLDNGIRNAV
                     IGNFNSVHSRTDIGALFENFIISERIKYLNYRRKNVSSYFWRTTQQQEIDYIEEEAGL
                     YSAYEFKWNPKKRVVMSKTFISNYQVVKEEVITPENFDSFLTKE"
     gene            15373..16407
                     /locus_tag="PL056_03090"
     CDS             15373..16407
                     /locus_tag="PL056_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015774478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HPM46035.1"
                     /translation="MKGSDFIVTSEILSLISEIDEFKGSWKALKNLKPELMSSLKLVA
                     SIESIGSSTRIEGVKLTDSEIEKLLKGLNPHSFSSRDEEEVAGYSEAMGMIFESFADI
                     DLTENHIKQLHQVLLKFSSKDVRHRGQYKKFPNHVEAFDRDGVSIGVVFETATPFETP
                     AKMEELVKWAHDNLNRSETHPLIAIAVFIVHFLAIHPFQDGNGRLSRILTTLLLLRSG
                     YEYIPYSSLEKVVEANKSDYYLALRQTQGDPEKNMSVWIIFFLKMMKKQKDELLEKLE
                     KEHSLMRLPELSIKILKEIKERGTATISEIELLTKANRNTIKAHLRQLVKDELLEMIG
                     FGKGARYRGR"
     gene            16501..16725
                     /locus_tag="PL056_03095"
     CDS             16501..16725
                     /locus_tag="PL056_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46036.1"
                     /translation="MLNQKEYVLKNFNFDKEVLELIKKLGVRNLSDFVRSAVDDKIRE
                     MQLQSLKERYQSEDMAEMTDDFDCTTGDGL"
     gene            16749..17081
                     /locus_tag="PL056_03100"
     CDS             16749..17081
                     /locus_tag="PL056_03100"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004073100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HPM46037.1"
                     /translation="MILRKGLIVLLSLDPVKGSEIAKTRPAVVVSNDVCNEYSPVVTI
                     IPCSTKIDKIYATEVLIKEGMDKPSKACADQIRTVDKTRIVKILGEISSTTMRQIENA
                     IMIHLDIR"
BASE COUNT         5204 a         3584 c         3245 g         5130 t
ORIGIN      
        1 ttgaaaaaag ctgccgcact ttcgaaaaga tctttttcaa tgttttcagg aacggatggc
       61 tcaccccgtg ttttctgagc attctgcaga attcaatatt agatctcaat attgcaaaaa
      121 gagatctgtt gcttcttcca cccaccctca ttgaaaggat attcttgtgc aaatacagcg
      181 acttaggatt ctgagaaaat agtctgaaca tgaaatctga atctgcggct atctcatatt
      241 gaaggtcata tctgccgaca cgatcataga gctctttttt tatgaaaagc gatgtgtgag
      301 gaggaaatat cccgttttca tgagaatcac tgccgatttg agcacttttc cagaccctga
      361 cagttttttc cacatcttca atcgaatgat aaactatatc tccgtaaacg agctttatat
      421 cattttcggt aattttttca actacttgcg acacaattga atttgagcag taaacatctc
      481 ctgcatgaag gagtgcaatt atttcacctg ctgccgcatc aattcccctg ttcatccctt
      541 cataaggcga agaatcagca ccttcgatga aaacatcaat tttttcaata tgttcccggc
      601 atttatcttt taatctctca ttttctgaag catcaacaac aatgtactca atattagaat
      661 aatcctgcat ctttactgac tgcatggttt ggaggaaccc ttttgcgtca tctttaacag
      721 ttgtaataat tgagactttc aatctttttt tcctatagcg aatttaaaaa cacctgcggc
      781 aatatttctc attttaagat atttccacct ttttccatac agtgccttct gaagattgta
      841 aggtttttca actttaaagc agttaagcct ttcatgtttt gtaataagtc cgaattcttc
      901 aattatttta aaatcatatc tatcaaaaaa agtattatcg agcattgtcc atatcaaaaa
      961 aagatggcag ttaagcatct gttcaagccc gtatttttca gcaatgctct taagcttttg
     1021 aagatcacag ttgccggcga caatataaaa ttcaagaagc cgtttgaaag taagatcggc
     1081 aaaatcaccc cttgaatagt catgaagaag cattatgaca gcggcatatt caagaggcgg
     1141 aacaatgaaa atctcaccgt cgggattgct tttctcttca cttttttcaa atatctcctc
     1201 gatgagcccc ggaaatttca caagagttat ccttgaatga atctcaacga gcgttgaatc
     1261 aaaatagaat gaaatatcct ctccaaaata ttttctcaag aaaaccggat agtttgactt
     1321 ataattaaac tcttgattat tattaacaaa ttcagctact ttgccaaatt catcagggtg
     1381 aacaagaatg tcaatatcag ccatctgcct tataccctga aaatcggaaa aaagggtgtt
     1441 taaaagcatc atccccttta caggcatgaa ctttgaaaaa ccgctctctt tcatctttga
     1501 agtaattttc cgcaaatggc tgtattttat gatatttgcc gcttcagttg caacatatcc
     1561 gcctttgaat gagggttgcc cacctttaat aaatcctatt gcttttaaat agggatcaga
     1621 ctcaagccct cgaaattctt ttcttaatgt tatttcagga aatacagtca tttaaccccg
     1681 ttcaaagatg aaaacttctg aattatacat ccatctgtct gtaaggaaag ctaaaaattt
     1741 gatttcgcct gagttttata taacatcatt tggctttacc atttttttgg gagcagaaac
     1801 atgtgtaatt tcaagacaat agttcctgaa gaagcaaaac tcaacccgtt ccttacactc
     1861 aagaacgaat ggggacttct taccgccgga aattttgaag acttcaacat gatgacaatt
     1921 gcatggggat atttcgggat aatgtggaac aagccggttt tcggcgctgt agtccgtccg
     1981 aacagatata cctatgaatt catggagaaa aacagcactt tcacagtatc tttcttccct
     2041 cctgaattca gggctgagct tgagttctgc ggctcaaaat cgggaagaga tgtaaacaag
     2101 gtttttgaga ccagcttcga accggttgac aacaacggat cggtctattt ttcacaggcg
     2161 gacattgttc ttgtctgcac gaagctatgt ttccaggatt ttgacccgga aaattttgtc
     2221 gaccccgaga taatcaagca ctatcccgaa aaatctttcc accgtcttta tatcggcgaa
     2281 ataactcaaa cacttcagaa ggaaaaacat aagaacaagg tgtctcattg aagggcttcg
     2341 ctgttttggt ttccaactgt gccggaagac aggaattgaa agccgaacac ggactttcat
     2401 tatttatccg ttacggtgaa aatgattatc tttttgacac aggcgggagt gatcttttca
     2461 ttgaaaacag ccagtttatg aaccttggaa ttgaaaacat aaagtgtgcc gttctcagcc
     2521 attttcactt ggaccacatt ggcggcatcc cttttttaag cagacatttt tcaatgatat
     2581 ccggaatttg tgatgtttat catcctgcgt tttctgaaat tctgaaattt cctgcattaa
     2641 gatccaatac tgtcagagaa tctcagaaaa ttgatgatga atgtgctctt gttctgtcaa
     2701 atgcagttaa agatgatgaa aatttcaacg aaatatcaat ggttgccgga aacacacttt
     2761 ttaccggatg cggccattca ggaattttga acattttaaa agaagcgcag aatttctcca
     2821 ctgtaacaac aatcgcaggg ggatttcata attttgacat gcagaaagat aaaatgaaaa
     2881 aaacagcttc agatcttttt ttactcggaa taaaaaaagt tattcttctt cactgttctt
     2941 cagtaaattc aataagatac ttcgaagatg ccgggatcga gtgcatattc ggaagtgtcg
     3001 gcaacagttt taacattgaa tgatggataa taatcaaatc tcaaaagttc tggaacactt
     3061 taatatagag tttgaaaaca cttcttcttt tgaaatttac ggagccccgt caagatcagc
     3121 tgacagagca gttgtcactg acaaaaataa tgagaaattc atcattgaaa aacaccattt
     3181 ctggaacatg agcaaaaagg ttaaaatagc tgaaaacatt aatcttttat acaataacgg
     3241 atttgaggaa tgctcgcctt atatttactc tgtgcaggga ccacctgtaa ttgaatttga
     3301 aaatagatgc tggatggttc agaaattcat tcatcacgat atattacaaa gacccgatta
     3361 tcttgatgac gggttgaaag gaactgctgc tgcagaaact ctccttaaaa tgtatgagat
     3421 ctcttcaaat ttaaaaatga acgatacgat tgactcgttt tctcttgctg aacacacttt
     3481 aaaaacagtc agaaagatcc gtaatatcca tcctgaaatt ttgagtaaaa tatctgatgt
     3541 aattaatatt attgaacatt catttatgcc tttaattgag gaaatgccgc ttgtattctg
     3601 tcacggagat tatcatccgc ttaacatttt atgggacgga aacaatctga aaaaagtgat
     3661 cgactgggaa ttttcaggtt acagaccaat gcttaccgac atcgcaaaca ttatcggctg
     3721 tgccggattt gaaaatatca gcgcttttga tagaaatttt gtgagaaagt ttttacaagt
     3781 tattgataag tatcattatt ttaaaagata tgattttaat gcaattatta tttttgtcat
     3841 agggttcaga ttttccggct ggcttaacga atggatcaaa gataaagacg gggcaatggt
     3901 tcaaagagag acagaatatc ttaattttct tagagaaatg atatgataag acctttaaga
     3961 tcttcagaca ttccggcaat agaaaaaatc tattcctttg caaacgaatc gatcggactt
     4021 ccggtaaata aagaccattt cagaaaggac gcaaaaaaat atgtatctga aactatttat
     4081 aaatgtgaga acatggtcta tgaacttaac ggagttccgg cagggataat ttcagtttca
     4141 tccgattata ttgaagggat ctttgttgat ccccgttttt tcaaaaaagg gatcggatca
     4201 gcacttataa aacattttct taacaaaaaa gaatacttgc aacttcaagt atatgaactg
     4261 aaccataacg ctctgaattt ttacagatct cacgggtttg tcatcacagg tggcggaata
     4321 tgccggatta cgggactccc ctattttgaa atggtttatg aaagaaaata atgtctacag
     4381 acacttacac tttgaactga tacactcacc aagaaaaatt ccgcacttgt agtaacagtc
     4441 aaaatcgttg acacactcgg gggctgtttc aggaagacac tcataacctg agtcatagta
     4501 accttcatcg catctgcagt agaaaatccc tttggcttcg acacattcac cgtgtccgga
     4561 gcaatcaata taatcacaat ttatctcttc aacagagaca catgtctttc cgttataaaa
     4621 atatccttca tcacatttgc agaccgctct tcccttttca gtttcacact ttccgtgctc
     4681 tccgcaatca actttttcgc atggatcggg cttatcttca atgcatcccc agccactttc
     4741 atcagccaga taacctgtct tgcattcaca tgttattgct gtgtagtcaa acccgcattc
     4801 tgaattcggc ccgcagtcaa cataatcaca cggatcgcct gaaacattct gaatacatgc
     4861 taaacctatc ggcagatacc cttcttcgca ggtgcagacc ggcttttcaa atacttcaac
     4921 ggcaacacac gaaccattgc cgctgcattc aactcctgaa cataaagatg ccggattgtt
     4981 agcaatacaa tcgggaaatt cgctgtgata tccgcttgga cagaaacatt caggaactcc
     5041 tccgttcaca aaacagaatc cgagatctga acaggttgca ttggcgcaat tcggtgcgga
     5101 agcatcttca tcagttgttt cgtcactatc tgcatccggc tctgtttcaa catctgtaaa
     5161 atcttcatca taattttcaa gatcttgatt ttcttcacta atttcgtcgt cgttctcaat
     5221 tgtatcatgg tctgtaacat cagttatttc aaaaggaatg tcttcgtctg accattttgt
     5281 ctcatcaact ttcatttcag aggaacagcc ccagaaaaaa agtaagacca acaaaaacgc
     5341 tgttatttta tccggcattt ttttctccag atattctgat agtttaaaca ctttttcgcc
     5401 tttaaaagtt gtttaataaa aaacaaaatc aagtcttgct gtcacaacac actccgaacc
     5461 gtcaatgctc tcaaaaacca tccttgagaa atgccctttt gcaaacgaaa cccttccttt
     5521 tgtgtcatgg ttgagaattt caacatttac tttacctgaa accgccctgt atccgctgtt
     5581 tccggcaacc tcgcctctta caaacataag aatgcagtca gtacagttct cccccatccc
     5641 gccggcaaga gtaagatcag aggttccctg tgaaagcgag aggagagaac cggttacaac
     5701 ttttcctgaa tttttcaagg cattttcgtc aagctggaat cttattccgc ccgttatatc
     5761 gcttgaaaac tgcttccttt catatttaaa atcttcgact cggcatctgt ttccgaactc
     5821 actttcaact ctgccgctgc acgagatcat aagaaaagta atgataacta actgaatttt
     5881 gttaatactc ataacttacc tccacagaaa ccgtatgtgc aagacggctg tagtcggcat
     5941 aatctccaaa gtcatcaccc acatttgaaa atgttttgtg aagaaaataa ccggcaagaa
     6001 tggaaaaatt gtcgttgata tcatagctga tctttgctga aaattctatt gtgctgtcta
     6061 ttctcacttt ttcatatatt gttgaaggga tagatacatc gctccagtaa actatcttgt
     6121 gagtatcgta gtcaaaagac cgggaaaaaa tgtatttcag tgaaacagga aatgaaaaag
     6181 aggagatctc ccagaaaagg ttcatgcccg agtaaagaga aaaatatctc ccttcgatcg
     6241 aaagttcacc gtaatagaca tcttcgtatc tgttataact gataaactga tctttaaaaa
     6301 aagttgcaga acttccggca aacacatcta acgcagcgtt ttcattaaag ttaaagcgtg
     6361 cccctccttc aaggaacaaa gaaggaccgg taaggtactc aacaagtgag ctggtggaaa
     6421 aataatatga aagacctgtc cttaagaaga tagcgtagat ctcgtcggga gcgtaaagat
     6481 atgaaaacgc aatgtacggg tcgacataaa gagatttcat ggatctgtaa tcacaggatg
     6541 aattgtttat tcctgcaatg aaagctatta tattgttttt attgacaaat tggatatatc
     6601 ccccttcaat tcccgaatca agaacagagt ttgaaacaga ttggagcgaa atgagtccgt
     6661 tcaaaaaaag atcaaatgca aatccgctct ctttttcgct ctgaacccct gcttcaaaag
     6721 aaaaaagcat cgttgccgag ctccttgtcg ccgatctgtt gaatatatcg gaagcgcttc
     6781 tgaatgaaaa tgacggatcc acatcacttt tccatccgcc cccgagagta acggacgcgt
     6841 tcgcttcata tgcagaaaca tcaaagaaaa caaaaaacag caacagaagt atgtatttga
     6901 attttctaaa agatccggct ttaataaacc cttctgtctt caatcctgcc cttccccttt
     6961 ctcttcttta gcaactcttt catttttctc attctttcgc ttctgtcaac atcatctgcc
     7021 aaattatcgg ggacttcttc taaactttca acttccattt cagtcatatg ttgatcaaca
     7081 acaggtattt tctcagtttt ttcaacagtt tgaggaacag catcctgttg agtgccggca
     7141 atttcctgtg gagaagtatt ctcaatttta ttttgactgc ggataactgt tctctttttc
     7201 tggtgccgga cctttttgat cttttttacc gtatcaatgt cagggatctc ctcgatatcg
     7261 gcaatttcat ccgcagcttc cggaacatct tctgatattt taaaaaattc tttctcccat
     7321 cccgatatag cgttttctga aagtcctttt tcaaatctca ctctgcttct aatttcagcc
     7381 ccttcacctt tcgcggcaag tggtgacaaa aaaagcagca ttgcggtaaa agcaataatt
     7441 ttcatatgtt atctgatagt ttttccagtt tatagagaac aacggcaact tttgaaccgt
     7501 ctgtgcggaa tgttataaga tgtctgtggc tcatcccttc aaaacgggct tccgtcacta
     7561 ttttccagga tccttcttca acagcggaaa cgacaaacgg gatctcattt tttccttttt
     7621 taagatcccc tttccagatg aaactgtcaa gattccggaa cttttcattt tgggaataga
     7681 atctcacccc tttatcgaga atgaaagaaa cttcaactcc ttgaatgtcc tgatctgatt
     7741 catactcaag aactattctt accggttcat taagctctgt ttccgttgaa gaaataaact
     7801 cagatccgtc atttccggtt cctgaaaaat aaaaaatccc gactgaaaag atcacggtga
     7861 aaaggaaagc ggaaacgatt tgaataatgg aaggtctgaa tttttttctg tcagcagaaa
     7921 tatccgcttt ctgaagtttt atgaaaagag cattatcaaa atcatccccg aatgtatcgt
     7981 cgagagtttt atcatatttt gagaaaaggg ctcttaattt ctgagcagaa gcataatatt
     8041 ttgaacaatt ctcacaactt gaaatatgtt ctaaaaatga gctttcatta agttcaatct
     8101 gttcatcaat cgctttaacg acatccctgc agtccatcac tcctcctcat catcgtctat
     8161 tgaacggaat cggtcggcag aaagttgtct ggtgtcagca tttccattta cgatctctac
     8221 aagttttttt ctccctttga aaaccctcga cttgagagct gtcagcgaaa tttcaagtat
     8281 ttcagccgct tcctcgtaac acatttcctg aatgtcaacg aggatcacag ccgttctgag
     8341 cttaagaggc agcaaagaga tcaggcggtg cacctcttct cttacatctg actttatcat
     8401 ctcgctttcg ctgttctccg gatcagggat ctcaatatct ttttcatcgg tggatattga
     8461 aaaagtatcc agtttattag aagggtctct gagatagttg atgaattcat tcttgattat
     8521 ccgccagagc catgttgtga aagaacctct gagtgcgaaa ttcttcattg aaatgaatgc
     8581 cttgacaagt gcttcctgaa gaatgtcgtc cgcaagagtt gcattaccgc aggcaatgtt
     8641 cactgaaaac gacctgagtt tcgctctgtg catggaaaca aggtcgcaga aagccctttt
     8701 gtctccctga gtcgccagtt ttatgagttc cttttcactg ttcttcaatt tagaaaagtc
     8761 ctgttatttt cccgttttcg tcaatatcca tcaaagccgc cgcgggtttt gcaggaagtc
     8821 caggcattgt cattatctcg ccggtgtata ctaccataaa accggcgcct gccgcatatt
     8881 ttatgtcagc tatttcaagt atgaaattct gcggactccc cttcagatcg gggttgtctg
     8941 aaaaagaata ctgcgttttt gccatgcaga cgggaacttc cgatatcccc catttttttc
     9001 tctgttcgat cttgttttcc acttttctcg gcacatgaat atctttggcg ccgtatatgt
     9061 tctttgcaat tttccttatc ttttcaaccg gctcatcttt aaaagaatat gtgtaattca
     9121 cttcaccgtg atcctgctga ctcagttcat aaaccgcctt tgcaagctca acgccccctt
     9181 tccccccttc agcccacaca tttgcctcaa aacactgcga accggcttct tttacaagat
     9241 ccttaatgat ctcaatctct tgtgttgtgt ccgagccgaa tctgttcaga gctacaacga
     9301 ccggacggtt gaaagaacgc atgttctcaa tgtgcttttt aagatttgca aacccttttt
     9361 ccactgccgt aacatccggc tcagaaagag atttgatctt aacaccgcca tgaaatttga
     9421 gagctctcac cgttgcgaca ataacaacaa catccggagg taccatccct tcactgcggc
     9481 tgacaagatt gaaaaatttc tcagcaccca gatcgcttcc gaaaccggct tcaattacta
     9541 ccatgtcggc atatcttctt gcaatgtcgg ttgctatcac tgaatttgtt ccgtgggcaa
     9601 tgtttgcgaa aggccctgta tggataagtg ccggatttcc ttcaagggtt tgaacaagat
     9661 tgggcattat cgcatcttta agaagtatcg ccagagcacc cgatgcatta agtccgccgg
     9721 ctctcaaggg aaccccgtca ttattttctg caattattat gtccgacaac ctttttttaa
     9781 ggtcagccat atctctgctc agaccgagga tcgccattat ttctgaggat gctgtgatat
     9841 caaaacggct ttctctcacc attccggcct ctttaccaag tccgataacc atgttgcgga
     9901 gtgccctgtc attcatatca agaacgcggt tccatacgat ttcccttgga agaagacatt
     9961 tctgctcaac gcgactgtaa tcattgtcaa gaagggctgt cagaaggttg tgtgccgaag
    10021 tgacagcgtg gatatcgccg gtaaaatgaa gatttatgga atccattgga atcacttgag
    10081 aaaacccgcc tcctgccgca ccgcctttca ctccgaaaac aggaccaagt gacggctctc
    10141 tcaaagttac tatcgatctt tttccgattt tgttgagagc ttctgacaac ccgatgctta
    10201 aagtagtctt tccctctccg gcgggagtcg gtgtcatcgc agtgacaagc accatttttg
    10261 ccttcggttc gtgcagatct ttggctttaa ggcagatctt gccgacattt ctcccccacg
    10321 ggatgacatg ctcactcttt aaacccattt tttctgcaat tttttcaatg ggttgctgaa
    10381 taacggaaga tgcaatttca taatctgtca acatttaaac cccctttgtc accattattt
    10441 gcttttttcc caaaatatct gaaaacgagc acaattacag caagttgaac aattataaga
    10501 ataaacggat tgacagaaaa tcctgccgga agatacccga agaaaaggga aacaagacct
    10561 attgcaaaag catacggcag ctgggttctc acatgctcga tgtgacttac tctgcatgac
    10621 atgcttgaca ttattgtcgt gtctgagatc ggagagcagt gatctccgaa gatagctccg
    10681 gtaagtattg cgcctgttac cgaaaaaatg aatgtttcca agttaataga cgcattctga
    10741 gccattgtta cggcaagagg aagagctgtc gggaaaagaa gcgccatggt cccccacgat
    10801 gtccctgttg caaaactcgc aacagatgct gtaacaaaaa caagtgcggg cagagaccag
    10861 accggaagtg ttcctgaaag aagaaaggag atgtattctg cggtcttaag ctctgtaaca
    10921 acggcactca aagcccatgc aagaacaagg attatcattg ccgtaaccat tgatttgacc
    10981 ccttccaccc acgatgaaac ggctgttgag accgagccgg tccggttaag tatcattgaa
    11041 acagcggcta cgatcgaacc gattagagac ccccatatca gaacacggaa ggaatctgcg
    11101 gaagcgatga tatccttgag tgtcggagac ccgccttcaa aagcagaata accgcttaca
    11161 attattccgg aaattgtcac aacgattaag gtcgttatcg gcagagccgc cgtataccat
    11221 ctgcattttg aagggtctat ctctttaaac gagctgaaat cttccggtgg ggcattttct
    11281 tcatctgcat atccgtccgc tgcttttttc tcggctttca acatcggtcc gaaatctctt
    11341 ttcatccaga taaagaccgg tatgaacaga agaagaagta ttgcataaaa tctgtatgga
    11401 agggaataga tgaaaacggt gtacggatca ctttctatcc ccgatgcctt gagagcgtca
    11461 cctataagtc ccacttcata tccgatccat gtgcttaaaa gtgcgacaga tgccaccgga
    11521 gcggtcgttg aatcaacaat aaagctcagt ttttcgcggc ttattttgtg gctgtcggtc
    11581 aaaggacgca tcgagtttcc gacaatgagc gtatttgcat aatcatcaaa aaaaatgaaa
    11641 agccccgccg cccatgtaag gatcagcgat tttgacgggg tgtctgtcct ttttgaaagt
    11701 gcggcaacta ttccgtgcat tccgcctgaa gctgttatga tgccgaccat tccgccgatc
    11761 atgagggaaa aaagaactat cattatgtgg gattcttcgt taagtccctt cacagcatag
    11821 ttgtctatcg tttttaaaaa tcctgaaaaa aatgaatagg ttaaagagtt gccgttattt
    11881 atgagaacaa tgaccgctcc tgaccataca ccgcagaaaa gagaaattat cacctgcttt
    11941 gtgaggaaag cgagccctat ggcaacaacc ggaggaatta ttgaaagtgt ctgtccgccc
    12001 tgaaaaatga tcaaaaaaca tataagaatg aaaaccgcgc aatatatgaa ttccggcttc
    12061 agcgatctca tagatccccg ggcgttttca tctttcttga agcgagatgc cgtctgtact
    12121 cctgatctat cgcttcagga tcaccgctgt tgtagacgac aacattcctc aaaaaaagat
    12181 atgagtcgat aggaaggtct gaaaactcaa gagcaagacc gtcatgatct tttcttacaa
    12241 caattccggt cagttctatt tcaccgacag ttgaatctga aatgatcttc actgtcaggt
    12301 tcacactttt tcccaaagca agatcaagat ctgtcgcaag gtatattccc gaaagactta
    12361 agtctctgac actacacact tttttcatgt cgttatagtt gatcagcgcg tcaactctaa
    12421 aatttacacg gctcattttt ctcttgctca tttttatctc ccgaccggct tacatatcaa
    12481 tatccatcga ttttctcatt ttcggaggat tcgcagaaac atacttgaaa ttcacttcca
    12541 caacatcctc tgattctcct tccggaaaaa tggtttctga aagttttttc acaacacaca
    12601 gaggtacttt cggatcggga aaagtgttgc cggtgatttt catatcttcg gcttttcctt
    12661 cttccgtgac aatgaatttt attgtgaaat ttcctcctat cccaggcgta acagcagccg
    12721 cttcctcata acaagtcctg aattttttca tttcagacag cgacagtatc attcttgatc
    12781 ttttatcgtc aggatcaatt tttttcttct gtttcagcgg cttgaaaacg agtgtgtagt
    12841 caagctctct gagttctccg ggcctgaacg ggagttcttt catatctttc gctatacatt
    12901 cagcgatttt gttaaaagaa gaagccaccc ttttgtatga tgctttctct acaaatcccg
    12961 actcgtcaat gataatgttg tattttattg tcatttcaga agcattcccg atcggtgtga
    13021 actgtttgta gcagtcaatg atctcaaggt caagctgctt cattttcttg ttcatttcct
    13081 tgtctgcagt ataacctctg atcgccactc tttcgccacc tttcatcgag gcctcgccaa
    13141 tttccggatc agattcagat tttgcttttt ctgcttttgc ctcactgctt gcacaggaga
    13201 aaatcgagaa gaaaaaagaa gcagcagcaa aaaaaagtat cagtttcagt ttcaaaacac
    13261 accctccgtt taattacccg gtaaagttta aacagctttg cttattagtc aaactttcgt
    13321 acttttacaa ataattaatt tcttgacaca aaccggcaca aaaggcatat ttcaccgttt
    13381 tttcaggagg attgaatgaa aaatctgata cttgcttccg gttcaccaag gcggcttgaa
    13441 cttctttcgt atttcggctt tgatctgaaa gtgataaagc cggatactga tgaaacagtt
    13501 gatcctgcct tgactccgca ggaagttgtc atttcgcttg ctcttcaaaa aggaaatgct
    13561 gtcgctcata tcgtcgggac aaaaactccg ctcttaagtg ccgacacgat agttgtcatt
    13621 gatgacgaga tcataaataa accggcggac aaagatgatg cgataaatat gatttcaaag
    13681 ctaagcggca gaactcattc ggtttttaca ggtgtagcaa tattttttaa tgacacagtt
    13741 gatacatttt ttgtcaaaag cgatgtaaca ttcaaaaaac tttcaatgaa agagatctca
    13801 gattactgcg acaccgatga gccgtatgac aaggcggggg cttatgcagt tcagggaatc
    13861 ggcgccttta tggtgaaaag catcaacggc tcatattcaa atgtgatcgg actgcctgtc
    13921 agcgaagtta ttgagcattt ccagaaactt aatattatta acggcttgaa ataacagtca
    13981 cccatattta tacccataaa tacccagaaa tttgattttc agtgcgtcaa aatcgaaaaa
    14041 tatcataata atttgcgatt ccattccaaa aatattataa taattttgga ttttggttta
    14101 tggaggagca gaaaatgata ggaagatatt taattgaaga gatcagaaaa aggatgttca
    14161 aggaaaaagc cattgtaata atgggggcaa ggcaggtggg gaaatccact cttgttgaga
    14221 tggtgcttga aaatttcagg aatgatcaca gaatactgaa acttaacggt gatgattctg
    14281 acacaagaac aatttttgaa aacccgactt caacaaagat cagaaatatt atcagcggac
    14341 acacgatcgt ttatattgat gaagcacaga gaattcccga gatcggactt gttttgaaaa
    14401 tcatggttga caacttcaat gatcttcagg tgatagcgac cggttcatca tcatttgaac
    14461 tttctggaaa aatcaacgag ccgcttacgg gaagaaaatt tgaatacact ctttttccgc
    14521 tctcttttga agaaatggtc aggcacacta actatcttga agaaaaaaga atgtttgaac
    14581 agcggcttat tttcggatca tatcctgata tcgtaaacaa caataacgat gcgggagatc
    14641 ttctgaagtg gcttgccgga agttatctct acaaagacat aatggcactg gaaaatattc
    14701 agaaaccggt gctgcttgaa aagatactta aagcgcttgc ccttcaagtc ggaagcgagg
    14761 tgagctataa cgaaattgct caacttgtag gtgctgacaa tgcaactgtt gaaaaatata
    14821 ttgaccttct tgaaagggcg ttcattgttt ttaagcttcc ggcactcact ggaaatgtga
    14881 gaaacgagat caaaaaagga aaaaagatct attttcttga taacggaatc agaaatgctg
    14941 tgatcggcaa tttcaattcg gtacattcaa gaaccgacat cggtgcactt tttgagaatt
    15001 tcataatctc tgaaagaata aagtatttaa attacagaag aaaaaatgtc tcaagctatt
    15061 tctggagaac tactcagcag caggaaattg attatattga agaagaggca ggtctttaca
    15121 gtgcatacga attcaagtgg aatcctaaaa aaagagttgt tatgtcaaaa acttttatat
    15181 caaattacca agttgtaaaa gaggaagtga taacacctga aaattttgac agttttctga
    15241 caaaagaata atacttataa tttttcggga tttgattcgc aagacccata tttataccca
    15301 taaataccca aaaggttgat tttcagcatt atttgggtat atttgggtat gtatttgggt
    15361 gtggaggttt gcatgaaagg ttcggatttt attgtaacca gtgaaattct ttcgcttatt
    15421 tcagaaattg atgaattcaa ggggagctgg aaagctctga aaaatctgaa accggagctt
    15481 atgtcttcct taaagcttgt cgcatcaatt gaatctatcg gttcttcgac gagaattgaa
    15541 ggggtgaagc tgacagattc tgaaattgaa aaactgctta agggattgaa tccacattct
    15601 ttttcttcaa gagatgaaga agaggtggcg ggatattctg aagcgatggg tatgatcttt
    15661 gaatcttttg ctgatattga cctgacggaa aatcacataa aacagcttca tcaggttctt
    15721 ttaaagttca gttcaaaaga tgtgagacac cgtggtcagt acaaaaaatt tccaaatcat
    15781 gtcgaggcgt ttgaccggga cggagtaagc ataggagtag tttttgaaac ggcaactcct
    15841 tttgaaacgc cggcaaaaat ggaagaactt gttaaatggg cacacgataa tttgaataga
    15901 tcagagactc atccgcttat tgcgatcgca gtttttatag ttcattttct tgcaattcac
    15961 ccttttcagg acggcaatgg acggctttca agaattctga caactcttct tctgctcaga
    16021 tccggctatg aatatattcc ctacagttca cttgaaaaag tggtggaggc aaataaatct
    16081 gattattatc ttgcacttag acaaactcag ggtgatcctg aaaaaaacat gtccgtatgg
    16141 ataatatttt tccttaaaat gatgaaaaaa caaaaagacg aactcctcga aaaacttgaa
    16201 aaagaacact ctttgatgcg actccccgaa ctatccataa aaatcttaaa agaaattaag
    16261 gaaagaggaa ccgccaccat cagcgaaatc gagcttctca caaaagccaa ccgaaacaca
    16321 atcaaagccc acctcaggca actcgtaaaa gatgaactcc tcgaaatgat cggatttgga
    16381 aaaggggcaa gataccgggg gaggtgaaat caaaacctgt tttaatcaca catggcttga
    16441 tatttataaa aacatttata taattattta cagaactgtg tttttttgat ggaggcgttc
    16501 atgttaaacc agaaagagta tgttttgaaa aactttaact ttgacaagga agttcttgaa
    16561 ttgattaaga aattgggtgt cagaaatttg agtgattttg tcaggtcagc ggttgatgat
    16621 aagattcgcg agatgcagct gcaatcactt aaagagcggt atcagtccga agatatggct
    16681 gaaatgacgg atgattttga ctgcacaaca ggcgacggtc tttagatttt tatatatgag
    16741 gtttaattat gatattaaga aaaggtctga tagttctttt atctcttgat ccggtcaaag
    16801 gatctgaaat tgccaaaacc cggccagcag ttgttgtgag caatgatgtc tgcaacgaat
    16861 attctccggt agttacgatt attccctgct ccacaaaaat tgacaaaatt tatgctacag
    16921 aagttttaat aaaagaagga atggataaac caagcaaagc atgtgcagac cagatcagaa
    16981 ccgttgacaa aacccgtata gtcaaaatac ttggcgaaat ctcttcaacc acaatgagac
    17041 aaatcgaaaa tgccataatg attcatcttg atataaggtg aatgttcagt aaattaaata
    17101 acttatattt ttgagggttt acataatgaa cattaatgat ttagggttta acggatattt
    17161 taa
//
LOCUS       DAOOYB010000031        16957 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_3289_length_16957_cov_11.194001, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000031 DAOOYB010000000
VERSION     DAOOYB010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 16957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16957
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3289_length_16957_cov_11.194001"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..2409
                     /locus_tag="PL056_03105"
     CDS             91..2409
                     /locus_tag="PL056_03105"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HPM46038.1"
                     /translation="MQENFDESLVSLYGEALDFAERMKSRQLTSMHFLLAYFSAPSEA
                     AVIFEEKGTSYKEIVDCYNEMVKMARHSGSALKEPVDTVKTLEESSLKKLLNKGQKVS
                     AVHFLASMTTARNSIAYRVLHKLEILTDIRLAAMKLIDEPAKRIKERAEEMRRESTRV
                     VPANAKKEKEKTAENTTETGTASNGRSEKRDDEILAMFGRNLTALAKNGSIDPVYGRS
                     REIESIVDILGRKRNNNPLIIGPAGSGKTAVVEAVALKQTEGLLPEKEIWELNLSSLV
                     GGTEYRGSLEKRIGDLIEFVEKNCERIIVFIDELHLLFSSGNDVVANMLKPALSRGTF
                     PLIGATTTAEFNKHIAKDPAMERRFTLVKVEEPKGEDLYRIVIHAAATLSKYHGVSMN
                     SEDIVRSAISLSNRYISGKSQPDKVLSLLDTVGSVLKRERRAEPSVEDLMSLVSDMTG
                     IPVESLLVDTSRIVKALPVILDRSIIGQVSAKEKIIRLLARRFGNRANGKPLASMIFA
                     GPTGTGKTEMAKKLASFLFGHEERLVVFDMSEFQELHSVSKLIGAPPGYTGFEDGGKL
                     TESFRREPYQLLLLDEIEKAHPKVLSIFLQILEEGRITDTRGFTADMTESIVIMTSNL
                     GAELFSISKVGFGDSGPAGVKDEDISNKIEQFLSPELLNRVDDVVVFRPFSDNELNSL
                     AKNYIDEVIYRASVNEQCFVTLENRDEASAYIVAKMSSKDKMMGARAVKRAVGRHFES
                     PLLEQCYSKKDPEISINITISITSEGLFTTVE"
     gene            2501..3748
                     /gene="pepT"
                     /locus_tag="PL056_03110"
     CDS             2501..3748
                     /gene="pepT"
                     /locus_tag="PL056_03110"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: protein motif:HMM:NF009920.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HPM46039.1"
                     /translation="MKRHQLAADIIKETRMLDRFLEYVKEDTQAVPGSETFPSSMKEK
                     NLGAKLVEELKGLGIDNAEMDENGYVYAKWESNCGVTKKVAFIAHMDVATDCAGEGVK
                     PVIHEKYDGSVIKLNGGTVIDPDDSEHLKNLIGDTVITSDGTTLLGADDKAGVAAIMS
                     MIEYLKKHPDIKRPEIRVCFTPDEEIGRGVDFINLKKVDAEFAYTLDGGTPYEVNYEN
                     FNAYGGKVTIKGVSIHPGYAKDKMVNAIRYAGKFIDMLPKTMSPERTHNREPFIHPVN
                     VTGGSEEVSVSMILRSFVPEDIDREKQIILNIIEVLKAEEPRLEVKAEFKESYLNMYE
                     IMKDKMYVYDYVKKAIEDLGATPDIHPIRGGTDGARLSFMGLPCPNIFAGGVNFHSQK
                     EFVALEDIGLAAAVAVKICENIK"
     gene            3806..6781
                     /locus_tag="PL056_03115"
     CDS             3806..6781
                     /locus_tag="PL056_03115"
                     /inference="COORDINATES: protein motif:HMM:TIGR04534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46040.1"
                     /translation="MKKIYMMFFILVFIFFSCHSEKNSSENDFDETGIDSDNLNDAVE
                     ESDLYDSEADEDTDKDEKSDDDSSGVITISKIPSNVVPDKPSYPYSFTKTENGILFFA
                     TNDATGIEPWITDGTETGTKILFDTCPGKMDSYSRLINSDGVQTIFYACGLIWASDGT
                     FEGTTVVSNSEDESLSFFAINEPEPLLESIQFQEKVFASIPVHDSSVYENPRWEIIII
                     DKELKTFTKFAEVNLFQKILGTKNGKIFFLAGNNDDKLHLWSSDGTKENTAPVVEISP
                     FNETDQWYVIDMAVKENFVFIISTSDYGDDFKFVVTDGAVTGTVIFDKIYESRGFVKI
                     EATDKNLYVSEYGGKFFHFDPSTGELEQIADLGNDFSASIYDMIAFGDSVFLLVSIND
                     IFEILKFDEKTGDFVSFHKLNEPENDYSMLPLFFKKDNTLCFVDNEKYRVFSSYQSYT
                     DVLNIYSVDEDKTREIKLNSPFFWEEDISYRDRPKNFDFIGNDIFFGGNEIIIPTSNS
                     WYHVGNEPKIIKEASAEAELLKNINHEVLSDYYAVYSPFFYDNILYYSTPNVSSGYPE
                     LNMTDHGANRKEKTVDIGVTDYIIFQENLYVNSTDAMFLVDHTSESVFTYVAGRAISI
                     FPLKDFILFSSSDSLHGYELWKTDGTEKGTEFFKDIFPGEEGSFPPEDFKAIENKMLF
                     RAKNSEETGWEPYFTDGTPENTKLLVDALEGWDSSDMFLMKVLDDYFYFVPYDQTADF
                     SRIYRTDGNNTELLADIVMSTEGFVRIMEPLKDKVVIITSNYQEVSVYSIDHKTTEFK
                     KTSSIPARFVWQEAFARIDNKVLFLVQLNSPGRQYELWITDGTATGTKKIKSLFQDLA
                     KTYNYKNYMFNSGGQVFFSISDGTHGDELWISDGTETGTKMVKDIMPGQGQSNPKIFL
                     EHKNILYFTASDGTHGNELWRTDGTTDGTYMVFDLFKGPDSSSINAIFFTDTDDIIVK
                     SYDEKDGYQLRKIPADICN"
     gene            complement(6814..7341)
                     /locus_tag="PL056_03120"
     CDS             complement(6814..7341)
                     /locus_tag="PL056_03120"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPM46041.1"
                     /translation="MKDSAKLKIIEKARDMLFTRNEEEITMIDVANELGITAPTLYHY
                     FSGKDELLAAGNSLITKEITAIAFIKFPSSMPAKMRIVTVASIVAEYFINSGLPVSYL
                     VEDPKDRPVVLKDFRDRITSLFAEYLKNKKAKHTPEKMAYRFLSALQSDMLLLRKNKE
                     KLEDDLAEKVFEDVV"
     gene            complement(7356..8048)
                     /locus_tag="PL056_03125"
     CDS             complement(7356..8048)
                     /locus_tag="PL056_03125"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HPM46042.1"
                     /translation="MIYLVRESLNIVDGFISVSDDEAKYLLKVRRLKKGDAVHFSTSG
                     VVYETVLDLDGRKPVFKIIRKTIGSHSSSRLFVAVASGDISAVEESVRNGVEAGADEF
                     FIFRSEFSNTDLSVVEKRMKRLKTIVVSAASQSRRGHLPEINFSTMEEIAGIDCEHIV
                     FHPYSPENILSYQPVDKREKMLWIGPEGGFSEKETAFFKKAGFKSFSLKTPILRMENA
                     VTFITAFIKNII"
     gene            complement(8045..8791)
                     /locus_tag="PL056_03130"
     CDS             complement(8045..8791)
                     /locus_tag="PL056_03130"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HPM46043.1"
                     /translation="MNKITIVVRTLEDFKETFIKNDEGKEITLPLKYIESPSEVSVGD
                     TVEMTLQIRNVQRTLDLAGEVRWKRLKDINLPGRRISAGLGIEFDEVSIELLKLHFKE
                     LFKELSNIDENMIGGNYIKVRTDIAEKYNIESRKASDFSEKRANPRITIMIPIEIFIK
                     NQTKKLNTVDISLNGMCVTTTEKLPVGEEVLVVFEDKSIRKQFLVKAVILRNVPEKDD
                     SASSYAVGFKFIFEDDRQKKELMKFIIKRA"
     gene            complement(8788..9672)
                     /gene="ftsY"
                     /locus_tag="PL056_03135"
     CDS             complement(8788..9672)
                     /gene="ftsY"
                     /locus_tag="PL056_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011460453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HPM46044.1"
                     /translation="MEDGLSKTKDGFISKLKNLFKTSVVDASLIDELEEILYTSDMGV
                     ETVSWLMSEVNQNRSSFQSGDDVKGFIKEKIRDVLAGTHREFQNRDLKPAVFLMVGVN
                     GAGKTTTIGKLASKFTGEGKQCVLAAADTFRAAAVEQLTVWGERTGCQVISDKEGADP
                     ASVAFNAINSGKSRGSDVVIIDTAGRLQNRVNLMDELSKINRVAGKALEGAPHETILV
                     IDANNGQNALSQAKQFGEAVNVTGIIVTKLDGTAKGGVLIGIAKELSLPVYFVGIGEK
                     VEDMRPFDPEEYTEALFS"
     gene            complement(10004..11401)
                     /gene="ahcY"
                     /locus_tag="PL056_03140"
     CDS             complement(10004..11401)
                     /gene="ahcY"
                     /locus_tag="PL056_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HPM46045.1"
                     /translation="MKDYKVRDISLADFGHKEIALAEIEMPGLMALIEKYGQEKPLKG
                     ARITGSLHMTIQTAVLIKTLKALGADVRWASCNIFSTQDHAAAAIADLGVPVFAWKGE
                     TLEEYWDCTLRALTFPEGLGPQMIVDDGGDATLLIHKGFQYENDNSIFDGKIDSNEMK
                     IIVDLIKREYLSDNNKWHRMAGDIKGVSEETTTGVHRLYQMLEAGELLFPAINVNDSV
                     TKSKFDNLYGCRESLADGLKRGTDIMVAGKTVVVCGYGDVGKGCARSMAGFGARVVVT
                     EIDPICALQAAMEGFEVRIIEEVISSGDIFVTATGNCDVIRTDHMEKMKNMAIVCNIG
                     HFDNEIQVDALYNLPGVKKINIKPQVDRIEFADGHSIILLSEGRLVNLGNATGHPSFV
                     MSNSFTNQVLAQIELWKGNHELGVYRLPKHLDEEVARLHLEKLGVSLTKLSEKQAKYI
                     GVCPKGPFKPDHYRY"
     gene            11569..12579
                     /locus_tag="PL056_03145"
     CDS             11569..12579
                     /locus_tag="PL056_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46046.1"
                     /translation="MKKLIFMIVVFSASIMFAESVKCENENGSCEFMDDGSFTCECSD
                     NSGWGGDGVAGSPSEEEVEMPTAEECLEWVDDMCGLPEGAETCENPAGHCIVYAEGYY
                     DCKCEDGSYGGSGGGESEPGDPGVSPEVDGEDSTEPQPVDEDEEVPDEYPTEECVADE
                     DCPEKFICVDGWCQFDYENVEKPVCAEVLVETCGTEAPDINNICDDKSFPYCSDAYTT
                     IMDKCEGVEIPADKIKELEEGKWNEYGRDIADCCRDYESAKPFIDDMLKCLETKSCEE
                     CYEQYEEVVDEEDKGNTGDSGDTSDSGNTDNEEGAGSNLEDDTPNEKSDGTSTGCSVV
                     AL"
     gene            12751..>13078
                     /locus_tag="PL056_03150"
     CDS             12751..>13078
                     /locus_tag="PL056_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone H1-like repetitive region-containing
                     protein"
                     /protein_id="HPM46047.1"
                     /translation="MAEKKAVAKKPVAKKAVAKKPVAKKAVAKKPVAKKAVAKKPVAK
                     KAVAKKPVAKKPVAKKAVAKKPVVKKAVAKKPVAKKPVAKKPVAKKPVAKKPVAKKPV
                     AKKAAPK"
     assembly_gap    13079..13178
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            13410..14750
                     /locus_tag="PL056_03155"
     CDS             13410..14750
                     /locus_tag="PL056_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006421030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPM46048.1"
                     /translation="MFERSIINRLNAWRNKPNRKPLILRGARQVGKTTVVKMFARQFK
                     HSIFLNMEDPHHRQLFKESLSFEERIDAVLLHGSIPKDADDILVFIDEIQSEPSAVAT
                     LRYFYETTPHIFVIAAGSLLEAAIDLKANFPVGRVEYLIMHPCSFKEFCIATGETELA
                     SILEKVPLPAYAHDKAMNVFKKYMLIGGMPEAVDVYAKTQDMIYVNSVYENLLTSFVD
                     DVEKYAKNGFVRVVRHIIKSAFAEVGSRIKFQGFGKSTYSSKDISESFDLLEKAMLMK
                     LVYPTSSVKIPIEPDLKKSPKLMLLDSGLLHYQSGLQSELFISEDPESVAFGRLVEHI
                     VGVMIYSGFSNPSAGLNFWAREKKQSNAEVDFVYQFKGKVIPIEVKSGATGKLRSLHQ
                     FVDQSPHQYAVRFCNATVSIENAVTIKGTEYKLLNLPWYLAEELENYLEWFVKK"
     gene            complement(14756..>15541)
                     /locus_tag="PL056_03160"
     CDS             complement(14756..>15541)
                     /locus_tag="PL056_03160"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013298849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HPM46049.1"
                     /translation="DAVFISVGSMTAGFTAGSMDKAPEKVAEKKESSWALWEKLAKIS
                     PEFGNPSVFNSDIDRSKWVSFTVTLKDQLFLQLLEKITKRKAGTEGPVTITDSNWLVS
                     FALPNHPHFKEQPENVNLLWGYGLRPDESGNFVKKKMSECTGREILTELLHHLKFEEH
                     LEKIINSSVCIPCLMPYITSQFMPRKIGDRPFVVPENSENFAFIGQYCEMPDDIVFTL
                     DYSVRSAQTAVYKLLKLNKLPTPIYKGHHNPLIVLRTIKTVFR"
     assembly_gap    15542..15641
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15642..16147)
                     /locus_tag="PL056_03165"
     CDS             complement(<15642..16147)
                     /locus_tag="PL056_03165"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: protein motif:HMM:NF017873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HPM46050.1"
                     /translation="MNRKAYIIGGGLSGLSVAFYLIKDGGFKGNDITLFEENDIAGGA
                     LDAVIHPEHDGYFMRGFRMLEQFVYSAVFDLMGDLPSLDDPHKTVLEEFNEFNEKVKT
                     LALSRLVKNGAAINARPFGMTFSDRMRVLKLLVTDESKLEKVRIDEYFSGSFFKSNLW
                     YEFSTTFS"
     gene            complement(16205..>16957)
                     /locus_tag="PL056_03170"
     CDS             complement(16205..>16957)
                     /locus_tag="PL056_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46051.1"
                     /translation="PDIDDASNEPCTYLFGRPNENTGLSWDQCKDFYNCEGVKFTAPV
                     YTESDIAILNSKIHLNPLPVLTSDPYETPELYPLEEEKACGLIWDTLNPDAYTLETFD
                     SIIEAELAGARVTHTGACGHCSSLQSLAVYIYQGDLATPVKKCTLDSILMGDDYLMEC
                     LQKLGFDENCAKIWMYNGKNTKKECMTTCLPLQNAVYHKSDGSLNDCIQCDEDKSGPV
                     FQAVSGRTRRNSGLPTALCRPCNTISPIDHHY"
BASE COUNT         5126 a         3392 c         3385 g         4854 t
ORIGIN      
        1 tgagtgaaat atatcgaatt aatttattgc aaaaaatgtc gggttggttt aatcttgtcc
       61 gcttattttg aggagtatcg ggagagatcg ttgcaggaaa attttgacga atcattggtt
      121 tctctttacg gcgaagcact cgattttgcc gaaaggatga aaagcagaca gcttacaagt
      181 atgcactttc ttcttgcata tttttcagct ccgagcgaag cggctgtaat ttttgaggaa
      241 aagggcacaa gttataaaga gatcgttgac tgctacaacg aaatggtgaa aatggcaaga
      301 cattccggaa gcgctttaaa agagccggtc gatactgtaa aaactcttga ggaaagctct
      361 ttgaaaaaac tactcaataa agggcagaaa gtttcggcgg tacattttct tgcctcaatg
      421 acaactgcaa gaaattcaat agcttacaga gttctgcaca aacttgaaat acttaccgac
      481 atccggcttg ccgcaatgaa actgatagat gagccggcaa aaagaataaa agagcgtgcc
      541 gaggagatgc ggagagaatc gacaagagtc gttcctgcaa acgcaaaaaa ggaaaaagaa
      601 aaaacagccg agaacacaac tgaaactggc acagcatcaa acggcagatc agaaaaaagg
      661 gatgacgaaa ttcttgcaat gttcggcaga aacctcacag ctcttgcaaa gaacggttca
      721 attgatcctg tttacggaag aagccgcgaa atagaatcga tcgttgatat tctcggaaga
      781 aaaaggaaca acaatcctct aataatcggc cctgccggaa gcggaaagac cgcagttgta
      841 gaagcagtcg cgctgaagca gacagaaggg cttctgcctg aaaaagagat atgggagctc
      901 aatctttcct ctcttgtcgg tggaacggaa tatcgcggct ctcttgaaaa aaggattggt
      961 gatctgattg aatttgttga gaaaaactgc gaaaggataa tagttttcat tgatgagctg
     1021 catcttcttt tctcttcggg caacgatgta gttgcgaaca tgcttaagcc ggcactttcc
     1081 cgtggaacat ttccacttat cggagcgaca acaacagctg aatttaataa gcacattgcc
     1141 aaagatccgg caatggaaag aaggttcact cttgtaaaag ttgaagaacc gaaaggcgaa
     1201 gacctttaca gaattgtcat ccatgcagcg gcgacactca gcaaatatca tggtgtcagc
     1261 atgaacagtg aagatatcgt cagaagcgca ataagtttga gcaaccgtta tatttcagga
     1321 aaaagtcagc cggacaaagt tctttcactt cttgatacag tgggaagtgt cttgaaaaga
     1381 gagcggcgtg cagaaccatc tgtggaagat ctgatgtcgc ttgtaagtga catgacagga
     1441 attccggtcg aaagtctcct cgttgacact tcaaggatag taaaagcact tcctgtgata
     1501 cttgacaggt cgataatcgg tcaggtgagt gcaaaggaaa aaataatccg gctccttgca
     1561 aggcgtttcg gtaacagggc aaacggcaaa cctcttgcct caatgatatt tgcaggtccg
     1621 acaggcacag gcaaaacgga aatggcaaaa aaacttgcat cttttctgtt tggacacgaa
     1681 gaacggcttg tcgtttttga tatgagcgaa tttcaggaac ttcactccgt ttcaaagctt
     1741 atcggtgcac ctccgggata cacagggttt gaagacggcg gaaagctcac cgaatcgttc
     1801 aggcgggaac cttatcagct tcttcttctt gatgagattg aaaaggcgca tcctaaagtt
     1861 ctttctatat ttctccagat acttgaagaa ggaagaatta cagacacacg cggctttaca
     1921 gcagacatga ctgagtcaat tgttatcatg acaagcaatc tcggagcgga acttttctct
     1981 atttccaaag tgggtttcgg cgattccgga ccggcagggg tcaaagatga agatatttcc
     2041 aataagatcg aacagtttct gtcccctgag cttttaaaca gagttgacga tgtagttgtt
     2101 ttcagaccat tttcagataa cgaacttaat tcccttgcaa agaattatat tgatgaagtg
     2161 atctatagag ccagtgtgaa tgaacaatgc tttgtaacac ttgaaaatag agatgaagca
     2221 tcggcttaca ttgttgccaa aatgagctcg aaagataaaa tgatgggtgc ccgtgccgtc
     2281 aaaagagccg tcggaagaca ttttgaaagc cctctgcttg agcagtgcta ttcaaaaaaa
     2341 gatcctgaaa tatcgataaa tatcaccatt tcaataactt cagaaggact ttttacaaca
     2401 gtagaatagt tgtccgataa ctggcttcct ctaaaattgc aatgaattta ttgtctgtta
     2461 taatgagccg ttgagttttt atcaatattt ggagggtatc atgaaaagac atcaattggc
     2521 agccgacatc attaaggaaa ccagaatgtt agaccgtttt cttgaatatg ttaaagaaga
     2581 cactcaggcg gttcctggaa gcgaaacttt cccttcttca atgaaagaaa agaatctcgg
     2641 tgcaaaactt gttgaagagc ttaaagggct tggaatcgac aacgctgaaa tggatgagaa
     2701 cggctatgtt tatgcaaaat gggaatcaaa ctgcggcgtt acaaaaaaag ttgctttcat
     2761 tgcccacatg gatgtcgcta cagactgtgc gggtgaagga gttaagccgg taattcatga
     2821 aaaatatgac ggctctgtaa ttaagctcaa cggcggaaca gttatagatc ccgatgattc
     2881 cgaacatctt aaaaatctga taggcgacac tgttataact tctgacggaa ctacacttct
     2941 gggtgctgat gacaaggcgg gtgttgccgc tattatgtca atgatcgaat atctcaagaa
     3001 acatccggac atcaaaagac ctgaaattag agtttgtttc actcctgacg aagagatcgg
     3061 cagaggtgtc gatttcataa accttaagaa agttgatgcc gaatttgcat acactcttga
     3121 cggcggaaca ccttatgagg ttaactacga aaattttaac gcatacggcg gaaaagtgac
     3181 aataaaaggt gtttcaatac atccgggata tgcaaaagat aaaatggtca atgcaataag
     3241 atacgcagga aaattcatag acatgctccc gaaaacgatg tcacctgaaa gaactcacaa
     3301 ccgcgaacct ttcatccatc ctgtgaatgt gacaggagga agcgaagaag tttcagtttc
     3361 catgatactt agaagttttg ttcctgagga catcgaccgc gaaaaacaga taattcttaa
     3421 catcatcgaa gttcttaaag cagaagaacc gcggcttgag gtcaaagctg aattcaagga
     3481 aagctatctc aacatgtacg aaatcatgaa agacaagatg tatgtatatg attatgtcaa
     3541 aaaagcgatc gaagatctcg gtgcgacacc cgacattcac ccgataagag gcggaaccga
     3601 cggtgcacgc ctttcattca tgggacttcc ctgccccaac atttttgcag gaggggtcaa
     3661 cttccactct caaaaagaat tcgtcgccct cgaagatatc ggacttgctg cggcagtagc
     3721 tgtaaaaata tgtgagaata tcaaataaga ctgctgaaga cattactact ggttttttaa
     3781 aaaatattag agagataaac aaatcatgaa aaaaatttac atgatgtttt ttatcctggt
     3841 ttttattttc ttttcatgtc atagcgaaaa gaattcgtcc gaaaatgatt ttgacgaaac
     3901 cggaatcgat tcagacaacc ttaatgatgc cgttgaagag agtgatcttt acgactctga
     3961 agctgatgaa gatactgaca aagatgaaaa gtctgatgat gactcttccg gggttataac
     4021 aatttcaaaa atcccctcta atgtggttcc ggataagcct tcctacccat attcttttac
     4081 aaaaacagaa aacggcatcc tcttttttgc cactaacgat gcaacaggaa ttgaaccatg
     4141 gatcactgat ggtactgaaa caggaacaaa aatattgttt gacacctgcc ctggaaaaat
     4201 ggattcatat tcaagactga taaacagcga tggtgttcag acaattttct atgcttgcgg
     4261 tctgatctgg gcatctgacg gaacctttga aggaacgact gtagtaagca actcagagga
     4321 cgaatctctt tccttttttg ccatcaacga acctgaacct cttctcgaaa gcatacagtt
     4381 ccaagaaaaa gtatttgcat ccattcctgt tcatgacagc tctgtatatg aaaatccccg
     4441 atgggaaata attataatag ataaagaatt gaaaactttt acaaaatttg ctgaagttaa
     4501 tctatttcaa aagatactcg gaacaaaaaa cggtaaaata ttttttctgg ctggcaataa
     4561 tgacgacaaa cttcacttat ggtcaagtga cggaactaaa gaaaacactg cgcctgtagt
     4621 tgaaatttca ccttttaacg aaaccgatca atggtatgtg atagatatgg ctgtgaaaga
     4681 gaactttgtt ttcataataa gcacaagtga ttacggggat gatttcaaat ttgttgtaac
     4741 tgacggcgct gttaccggaa ccgtgatctt tgataaaatc tatgaaagta gaggattcgt
     4801 taaaattgaa gcaactgata aaaacctcta tgtatctgaa tacggtggca aatttttcca
     4861 ttttgatccc tcaaccggag aacttgagca aattgcagat ctcggcaacg acttttccgc
     4921 aagtatttat gacatgatag cttttgggga ttctgttttc ctccttgttt caataaatga
     4981 tatttttgaa atattgaaat ttgacgagaa aaccggcgat tttgtctcat ttcataaatt
     5041 aaatgaacca gaaaatgact atagcatgct tccgctcttt tttaagaagg ataatactct
     5101 gtgctttgta gacaacgaaa aatacagggt tttctcatcg tatcagtcat atactgatgt
     5161 tttaaacatc tacagtgtag atgaagataa aactcgtgag atcaaattaa acagcccttt
     5221 tttctgggaa gaagacatat catacagaga tcgtcccaaa aattttgact tcatcggaaa
     5281 cgacatcttt tttggcggta atgaaataat aattccaact tccaacagct ggtaccatgt
     5341 gggaaacgag ccaaaaataa taaaagaagc atctgcagaa gccgaattgt taaaaaatat
     5401 aaatcatgaa gtgctttcag attattatgc cgtttacagt ccgttctttt atgacaatat
     5461 tctctattac agcactccta atgtttccag cggatacccc gaattgaata tgactgatca
     5521 cggagccaac cgcaaggaaa agactgttga tatcggtgtt actgattata tcattttcca
     5581 agaaaacttg tatgtaaatt caacagacgc aatgtttctg gtggatcata catcggaatc
     5641 tgtttttacc tatgtagcag gtcgggcaat ttccatattt cctcttaagg acttcatctt
     5701 attcagttca agcgactctt tacacggata tgaactctgg aaaactgacg gaactgaaaa
     5761 aggaacagag tttttcaaag acatctttcc aggtgaagaa ggaagttttc cgccggaaga
     5821 tttcaaagcc attgagaata aaatgctttt cagagcaaaa aacagcgaag aaacaggatg
     5881 ggaaccatat tttactgacg gaactcctga gaatacaaaa cttcttgtag atgctttgga
     5941 aggatgggat tcttcagata tgttccttat gaaagtgctt gatgattatt tttattttgt
     6001 tccatacgat cagacagcag acttttccag gatttataga actgatggga acaatactga
     6061 actgcttgca gatattgtca tgtcaaccga agggtttgtg agaattatgg aaccgttaaa
     6121 ggacaaagtt gtcataataa caagcaatta tcaggaagtt tcagtatatt caatcgacca
     6181 caagactact gagttcaaga aaacatcatc gatcccagca agattcgttt ggcaggaagc
     6241 attcgccaga attgacaata aggttctatt tcttgttcaa ctaaacagtc ctggaaggca
     6301 gtatgaattg tggataacag atggaacggc aactgggacc aaaaagatta aaagtctttt
     6361 tcaagatctc gcaaaaacat ataattataa aaactatatg tttaattcag gcggtcaagt
     6421 gtttttttca atctctgatg gaactcatgg agatgaatta tggataagcg acggcactga
     6481 aaccggaaca aaaatggtaa aagacattat gccggggcaa ggtcaatcaa atccgaaaat
     6541 ttttcttgaa cacaaaaaca tcctttattt tactgcgtct gacggaacac atggaaatga
     6601 gctctggaga actgacggca ctacggacgg aacatatatg gtcttcgact tatttaaagg
     6661 tcccgattct tcctcaatta atgcaatctt ttttactgac actgatgaca taattgtaaa
     6721 aagttacgac gaaaaagacg gatatcaatt aagaaaaata ccggctgata tctgtaattg
     6781 atttattgct ctttttccgg aaatataaaa ttatcagaca acatcttcaa aaactttttc
     6841 agcaagatca tcttcaagct tttctttgtt ctttcttaaa agcaacatgt ccgactgaag
     6901 cgctgaaagg aatctgtatg ccattttttc aggggtgtgt ttcgcctttt tatttttaag
     6961 atattcagcg aacagagatg ttatcctgtc tctgaaatct ttcagaacta ccggcctgtc
     7021 cttgggatct tcgacaagat atgaaacagg cagacctgaa ttgataaaat attctgcaac
     7081 tatgctggca accgtaacaa ttctcatttt tgcaggcatg gatgagggaa attttatgaa
     7141 tgctatggct gttatttctt tcgttatcag actgttcccg gccgcaagaa gctcatcttt
     7201 gcctgaaaaa taatgataca gtgtcggggc tgttatcccg agctcgttcg caacatctat
     7261 catcgttatc tcttcctcgt tgcgggtgaa aagcatgtct ctcgcctttt caatgatctt
     7321 aagttttgca gaatctttca tcgtgatctc ctttgtcaga ttatgttttt aatgaacgcc
     7381 gttataaatg taactgcatt ttccattctg aggatcggtg tttttagtga gaacgattta
     7441 aatcccgcct tcttaaagaa cgccgtctct ttttctgaaa atcccccttc cggacctatc
     7501 cagagcattt tctctctttt gtccaccggc tgataactta agatgttttc cggtgaataa
     7561 gggtgaaaca caatatgctc acaatctatt ccggcaattt cctccattgt cgaaaaatta
     7621 atttcgggaa gatgccctct ccttgactga ctggcggctg aaacaacaat ggttttcagt
     7681 cttttcattc ttttttcaac aacagaaaga tctgtattgg aaaactccga tctgaagatg
     7741 aaaaattcat cagcacctgc ttcaacgcca ttccttaccg attcctctac agcactaatg
     7801 tctcctgatg caacagcaac aaaaagtctg gatgaagaat ggcttcctat cgtttttctg
     7861 attattttaa aaaccggttt tctgccatca agatcaagaa cggtttcata gacgaccccc
     7921 gaagttgaaa aatggacagc atcacctttt ttaaggcgtc ttactttaag cagatacttt
     7981 gcttcatcat ctgaaacaga aataaaaccg tcaactatat taagggattc tcttacaaga
     8041 tatatcatgc ccgcttaatg atgaatttca tcagctcctt cttttgtctg tcatcttcaa
     8101 aaatgaattt aaatcctact gcataacttg aagcactatc atctttttcc ggcacatttc
     8161 tgagaataac agcttttaca aggaactgct tacggatgga cttatcttca aacaccacga
     8221 gaacttcttc accgacagga agtttttcag tggttgtaac acacattccg ttcagagaaa
     8281 tatcaaccgt gttgagcttt tttgtctggt tcttgataaa tatttcaata gggatcatta
     8341 ttgttattct tggatttgcc cttttttcgc tgaagtccga cgccttcctg ctttcaatgt
     8401 tgtatttttc agcaatatca gttctgacct tgatgtagtt cccgcctatc atgttctcat
     8461 ctatgttgct cagttccttg aacagttcct tgaagtgaag cttcagaagt tctatggaaa
     8521 cttcatcaaa ttctatacca agacctgcgc ttatccttct tccaggcagg ttgatatcct
     8581 ttagccgctt ccatcttact tcgcctgcaa gatcaagagt cctttgaaca tttcttatct
     8641 gaagggtcat ttcaactgta tcgccgacag aaacttcgga aggactttca atatacttaa
     8701 gcggaagggt tatctctttt ccctcgtcgt ttttaatgaa tgtctccttg aaatcttcaa
     8761 gtgttctgac aacgatagtt atcttgttca tgaaaacagc gcctccgtat attcttcagg
     8821 atcaaaaggt ctcatatctt caactttctc acctatgcct acaaaatata cagggagcga
     8881 taattctttg gctattccta tcagaacacc cccttttgca gtaccatcaa gctttgtcac
     8941 tattattccc gtcacattta ccgcctcacc gaactgcttt gcctgggaaa gtgcgttctg
     9001 tccgttattt gcgtctatga caagaatggt ttcatgcgga gcaccttcaa gggccttgcc
     9061 tgccactcta ttaatttttg aaagctcgtc catcaggttc actctgttct gaagacgacc
     9121 tgccgtatct attataacaa catctgatcc tctgctcttt ccactgttta ttgcattgaa
     9181 agccaccgat gcaggatctg caccttcttt atcgcttatt acctgacatc cggttctctc
     9241 accccagacc gtcaactgct ctaccgccgc cgctctgaat gtgtccgcag cggcaagaac
     9301 acactgtttc ccttcaccag taaatttgct tgcaagcttt cctatcgtag tcgtctttcc
     9361 tgctccgttt acgcctacca tcaaaaaaac agccggcttc agatcacgat tctgaaactc
     9421 tctgtgtgtc ccggcaagca catcccttat cttttccttt atgaaacctt taacatcatc
     9481 tccgctctgg aatgaggacc ggttctgatt gacctcactc ataagccagg aaactgtttc
     9541 aacacccatg tcggatgtgt agaggatctc ttcaagttca tcgataagcg atgcatcaac
     9601 cactgaggtc ttgaaaagat ttttgagctt tgaaataaag ccgtctttcg tttttgagag
     9661 tccgtcttcc atcgttgcat gctttgattc agaaacatgt tccttcggtt gatcttcaag
     9721 ttttatttca atggttttga acggaccgga acctcttgta agatcgatcg ttttcaacgg
     9781 gactctttgt gctttaactt caggcgttat tggtggctta atttcttctt tctttttgtc
     9841 agggatcaat aagacgatta gaagcattat cagaagaaag gcgggaatga tgtaaaagaa
     9901 cagctcaccg ttcttataac ttaattcgat ccaggataga atactcactg caaacctctt
     9961 aacaattcag gaaaaaatac atttttatcg taaaaaagtc aagttaatac ctgtaatggt
    10021 cgggcttaaa agggcctttt ggacagacac ctatatactt tgcctgcttt tcggaaagtt
    10081 ttgtcagcga gactcccagt ttttcaagat gaagtcttgc aacttcctca tcaagatgct
    10141 tgggaagacg gtaaacgccg agttcatggt ttcctttcca gagttcgatc tgtgcaagaa
    10201 cctgatttgt aaatgaattg ctcattacga aactcggatg ccccgttgca tttccaagat
    10261 taacaagccg tccttcgctc agaagtatta tcgaatgacc gtcagcaaac tcgatcctgt
    10321 caacctgcgg tttgatattt atcttcttta caccaggcag attataaagg gcatcgacct
    10381 gaatttcgtt atcaaaatgg ccgatgttgc acactatcgc catgtttttc atcttttcca
    10441 tatgatctgt tcttataaca tcacagttgc ctgtcgcagt tacaaaaatg tctcccgacg
    10501 aaatgacctc ttcaatgatc cttacctcaa atccctccat agccgcctga agagcacaga
    10561 tcggatcgat ctcagtaaca acaacccttg caccgaatcc cgccatcgac cttgcacatc
    10621 ctttccctac atcaccgtaa ccgcagacaa caacggtttt accggcaacc attatgtcgg
    10681 tacctctttt aagaccgtcc gccagcgact ctctgcagcc gtagagattg tcaaacttcg
    10741 actttgtaac cgaatcgttg acatttattg caggaaaaag aagttcgccg gcttcaagca
    10801 tctgatagag acgatgtaca cctgttgtcg tctcttcgct tacccctttg atgtctcctg
    10861 ccatgcgatg ccatttatta ttgtctgaaa gatattctct ctttataaga tcaactatta
    10921 ttttcatttc gttgctatcg atctttccgt caaatataga attgtcattt tcatactgga
    10981 accctttgtg tattagaagt gtcgcatctc ctccgtcatc aacgatcatc tgtggcccta
    11041 gcccttccgg aaaggtgagc gccctgagtg tgcagtccca gtattcttca agagtctctc
    11101 ctttccatgc aaaaacagga acgccgagat ctgcaatggc agcggcagca tggtcctgag
    11161 ttgaaaaaat gttgcagctc gcccatctta catcggcacc gagagctttc aatgtcttga
    11221 ttagtaccgc agtctgaatt gtcatatgca gacttcctgt tatccttgca cccttgagcg
    11281 gtttttcctg tccgtatttt tcaataagtg ccatcaatcc cggcatctct atttctgcaa
    11341 gagctatttc tttgtgtccg aaatcagcaa gtgaaatgtc cctgactttg taatctttca
    11401 ttttttcctc gtattgtttt taatgtttca aagaacctga attaatatca tatcaattat
    11461 atatagtcaa tcgggaggca tttttttcgt gaaaaaattt tttttctaaa taaaattgtc
    11521 cgggattgtg aactaaatca ccgtaatttt tacccacagg aggggataat gaaaaagctg
    11581 atctttatga ttgttgtttt ttcagcatca ataatgtttg ctgaaagtgt aaaatgcgaa
    11641 aacgagaatg gaagttgtga atttatggat gacggaagct ttacctgtga atgctcagac
    11701 aactccggct ggggcggaga cggtgtcgca ggaagtccat cggaagaaga agttgaaatg
    11761 ccgactgctg aagaatgcct tgaatgggtt gatgacatgt gcggacttcc ggaaggggct
    11821 gagacttgtg aaaatcctgc aggacattgc attgtctatg ctgaaggata ttacgactgc
    11881 aagtgtgaag acggaagcta tggcggatca ggcggcggag aaagcgaacc cggcgatccc
    11941 ggtgtatctc ccgaagtaga cggtgaagac agcactgagc cacagccggt tgatgaagat
    12001 gaagaagttc ctgacgagta tcctacagaa gaatgtgtag cggatgagga ctgtcctgaa
    12061 aaattcatct gtgttgacgg ctggtgccag ttcgattatg aaaatgttga aaaaccggtt
    12121 tgcgctgaag ttcttgttga aacatgcgga acagaagctc cggacatcaa caacatatgt
    12181 gatgacaaat catttccgta ttgctcagat gcttacacaa ccataatgga taaatgtgaa
    12241 ggggttgaaa ttcctgctga caagatcaaa gagcttgaag aaggaaaatg gaacgaatac
    12301 ggtagagata ttgcagactg ctgcagagat tatgaatctg caaaaccttt tattgatgac
    12361 atgctcaagt gtcttgaaac aaaaagctgt gaagaatgtt acgaacagta tgaagaagtt
    12421 gttgatgaag aagacaaagg caacacgggt gattcaggtg atacaagcga cagcggcaat
    12481 actgacaatg aagaaggagc cggatctaat cttgaagatg acacaccaaa tgaaaaaagt
    12541 gacggaacat caacgggctg ctcagtagtg gctctctgat atttttcaaa aaaattttct
    12601 gcaacttttt ccggacaaaa aaatatctcc gcaatttttt taagtatttt gtatcatttt
    12661 cgttgactat ggaaatagtt tagtttaaat tataaacaaa tgcgttttat acagcgtgtg
    12721 ttttattcat ttttttgtgg agagaaaaaa atggcagaaa aaaaagcagt agctaagaaa
    12781 ccagttgcta aaaaagcagt agcaaagaaa cctgttgcta aaaaagcagt agctaaaaaa
    12841 cctgttgcta aaaaagcagt agctaaaaaa cctgttgcta aaaaagcagt agcaaagaaa
    12901 cctgttgcta aaaaaccggt tgctaaaaaa gcagtagcta aaaaacctgt tgtaaaaaaa
    12961 gcagtagcta agaaacctgt tgctaaaaaa cctgtagcaa agaaacctgt tgctaaaaaa
    13021 ccagtagcaa agaaacctgt tgctaaaaaa cctgttgcta aaaaagccgc tccaaaaann
    13081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngt agcaaagaaa cctgttgcta
    13201 aaaaacctgt tgctaaaaaa gccgctccaa aaaaagccac aaaaaaatag ttcaactttt
    13261 taacccttaa ataaccaaat atttttttat aaacactaac ttttcttcat ttatccctga
    13321 ctttagggag aattatgcca aaattatggt tgactttagg aaaaaaaaga tgagaattat
    13381 ggctgacttt aggaattttg gaggcgtaag tgtttgaaag atctattata aatcgtctaa
    13441 acgcgtggcg gaacaagccg aatcgaaaac cgttaattct gagaggtgcc cgtcaggttg
    13501 gaaaaacaac tgttgtaaag atgttcgccc gccagtttaa acactctatt tttctaaaca
    13561 tggaagaccc gcaccatcga caacttttta aagaatcatt atcttttgaa gagcggatcg
    13621 acgctgttct tcttcatgga tctattccaa aagatgcaga tgatatactc gtttttattg
    13681 acgagatcca atcagaacct tcggcagttg caactttacg atatttttat gagacaactc
    13741 cgcatatttt tgttattgcc gcaggttcgc ttcttgaggc ggcaattgac cttaaggcga
    13801 atttccctgt cggaagagtt gaatatctca ttatgcatcc ctgctctttt aaggagttct
    13861 gcattgcaac aggagaaaca gagctggctt ctattttaga aaaagtccct ctacctgcct
    13921 atgctcatga taaagcgatg aatgttttta agaaatatat gctcataggc ggaatgcctg
    13981 aagcagtcga tgtttatgca aaaacacagg atatgatata tgtaaattca gtgtatgaaa
    14041 atcttttaac atcttttgtt gatgatgttg aaaaatatgc gaagaacgga tttgtcagag
    14101 ttgtccgtca cattataaaa tcagcgtttg ctgaagtcgg cagcagaata aaatttcagg
    14161 gtttcgggaa gtcaacttat tcgtcaaaag atataagtga atcatttgat ctgcttgaaa
    14221 aggcaatgct tatgaagctc gtttatccca caagttcagt gaaaattcca attgaacctg
    14281 atctgaaaaa atcacccaaa ctgatgcttc tggacagcgg actgcttcat tatcagtcag
    14341 gtcttcaaag tgaacttttt atttccgaag atcctgaatc ggtagctttc ggaagacttg
    14401 ttgaacacat cgtcggcgtt atgatatatt cagggttttc caatccttct gccggattaa
    14461 atttctgggc aagagaaaaa aaacagagca acgctgaagt tgatttcgta tatcagttca
    14521 aaggaaaagt cattccgatt gaggttaaat caggagcaac agggaaactc cgctcactgc
    14581 atcagtttgt agatcagtca ccgcatcaat acgcagtcag attctgcaac gcaactgttt
    14641 caattgaaaa cgccgtcacc atcaaaggca cagaatacaa acttctgaat ctcccgtggt
    14701 atcttgccga agaactcgaa aactatctgg aatggttcgt gaaaaaataa atttgctatc
    14761 taaacacagt ctttatcgtt cttaaaacga tgagcgggtt gtggtgtcct ttgtagatgg
    14821 gtgtgggcaa tttattgagt ttgaggagtt tataaactgc ggtttgtgct gatctcacag
    14881 agtaatcaag tgtgaacaca atgtcatcag gcatttcgca gtattgtccg ataaacgcga
    14941 aattttctga attttccggc actacaaaag gtctgtcacc aattttgcga ggcatgaact
    15001 ggcttgtgat ataaggcatc agacatggaa tacagacaga tgaattgata attttttcaa
    15061 ggtgttcttc aaatttcagg tgatgaagaa gttctgtgag tatttctctt cctgtgcatt
    15121 cactcatttt cttttttaca aaatttcctg attcatccgg tcgaagtccg tatccccaca
    15181 agagattgac attctccggc tgttctttga aatggggatg attcggaaga gcaaaagaga
    15241 ctagccagtt tgaatctgtt attgtaacag gtccttctgt tcccgctttt ctttttgtga
    15301 tcttttcaag cagttgtaaa aaaagctgat ctttgagagt aacagtgaat gaaacccatt
    15361 ttgatctatc gatatcgcta ttgaaaactg aaggatttcc gaattcgggc gaaatttttg
    15421 caagtttttc ccaaagagcc catgaactct ctttcttttc tgcaaccttt tcgggagctt
    15481 tatccattga tccggcagta aaaccggccg tcattgatcc aacagaaatg aaaacggcat
    15541 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naatgaaaaa gtagttgaaa
    15661 attcatacca gagattgctc ttaaaaaaag aacccgaaaa atattcatca atacggactt
    15721 tctcaagttt tgattcatcg gtgacaagaa gtttgagcac tctcattctg tctgagaaag
    15781 tcattccgaa agggcgggca ttgattgcag ctccattttt tacaagtcgt gaaagcgcca
    15841 gcgtttttac tttttcatta aactcattaa actcttcaag aacagtttta tgcggatcat
    15901 caagagatgg aagatcaccc atcagatcaa aaacagccga gtatacaaac tgttcaagca
    15961 ttctgaatcc gcgcatgaaa taaccgtcat gttcaggatg tataacggca tcaagcgctc
    16021 cgcctgcaat atcgttctct tcaaaaagag taatgtcatt tcctttaaat ccgccatctt
    16081 ttatgagata aaacgcaaca gacaatcccg aaagcccgcc accgataata tatgcttttc
    16141 tattcatagt cctcaaaaac aaaaaaccgg agggattata tctgtgaaaa gggatgttgc
    16201 aatgttaata gtgatggtct atcggagata ttgtgttgca cggtctgcaa agagctgtcg
    16261 gcagtcctga gttgcgacgg gttctccctg aaaccgcctg aaacaccggt ccgcttttgt
    16321 cttcgtcaca ctggatacag tcattaagcg atccgtcaga tttgtgataa accgcatttt
    16381 gaagagggag acaggtggtc atacactctt tttttgtatt ttttccgtta tacatccata
    16441 tttttgcaca gttctcgtca aaccccagtt tctgcagaca ctccataaga taatcatccc
    16501 ccataagaat gctgtcaaga gtgcatttct ttacaggtgt tgcaagatct ccctgataaa
    16561 tataaaccgc taagctctgc agagatgaac agtgaccgca cgccccggta tgtgtaactc
    16621 tcgctccggc aagttccgcc tcaataatac tatcaaaagt ttcaagcgta tatgcatcag
    16681 gattgagcgt atcccatatc agtccgcatg ctttttcttc ttcgagcgga taaagttcag
    16741 gtgtttcata agggtctgaa gtaagcaccg gcagaggatt aaggtgtatc ttggaattca
    16801 gtattgcgat atcagattca gtatataccg gtgctgtaaa tttcacccct tcacagttat
    16861 aaaaatcttt gcactggtcc caagaaagcc cagtgttttc attgggtctg ccgaaaagat
    16921 aggtgcacgg ctcattgctc gcatcgtcaa tatcagg
//
LOCUS       DAOOYB010000032        16897 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_3313_length_16897_cov_13.141670, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000032 DAOOYB010000000
VERSION     DAOOYB010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 16897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16897
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3313_length_16897_cov_13.141670"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1356
                     /gene="hutH"
                     /locus_tag="PL056_03175"
     CDS             <1..1356
                     /gene="hutH"
                     /locus_tag="PL056_03175"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HPM46052.1"
                     /translation="TGFGILSDVKIAHENLEELQINLIRSHAIAVGTPYDKETVRAIM
                     VLRANVLAKGYSGITLETFDKLLEIINKNIVPFIPCQGSVGASGDLAPLAHLALVLVG
                     EGFVINEHGEKVDTAVIFKEKGITPVVLKAKEGLALINGTQAMTAIGCLTVSRVRNLL
                     KLADIAVTSTLEGIMGTDRAFDRKVNVVRAHRGQMEVSENVLRIIKDSEIRDSHTCCS
                     KVQDAYSLRCAPQVHGAVRDALRHAESIITTEINSSTDNPLIFPEEKETISAGNFHGE
                     PIGLVMDYLTAAVSEIGSISERRIEQMINPVFSQAPAFLVKEKGLNSGFMIAHVTAAS
                     IASENKTLCFPATVDSIPTSAGKEDHVSMGTTAARKCAQVVDNTVKIICMEIFTAMQA
                     IDFRKPLIPGAGVKAAYELIRKTVPFMEKDRFMYPMFENVLKNEYLIVEEVEKVTGVL
                     N"
     gene            complement(1369..1986)
                     /locus_tag="PL056_03180"
     CDS             complement(1369..1986)
                     /locus_tag="PL056_03180"
                     /inference="COORDINATES: protein motif:HMM:NF013378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HPM46053.1"
                     /translation="MIERYKTVFEQAGPVEIKEKGSRFISYCFHVKTKDDIDNILKTQ
                     KKEHYDSTHVCYGYILGEGEIKLFRYSDDGEPSGTAGLPIYNEILRKELFNVLVTIVR
                     YYGGTKLGTGGLTRAYGGSAREVLEKAAIATVEIKKTVTVEASFDLTGLVMNHISHFS
                     GIEIISNSYNEKGILLEIFIPIGTVEKFKDDLIEKSGGKIVFHKP"
     gene            complement(1983..2270)
                     /locus_tag="PL056_03185"
     CDS             complement(1983..2270)
                     /locus_tag="PL056_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46054.1"
                     /translation="MPKIEMNFQSSKQDKEIWEWLENNLKHILEKKIGVEKIEVIPDK
                     NSKKIEFKGRTVRGTLVLNNGCVSIVVEIPLLYRFFTPHIRSAVEKIFDQI"
     gene            complement(2276..4654)
                     /locus_tag="PL056_03190"
     CDS             complement(2276..4654)
                     /locus_tag="PL056_03190"
                     /inference="COORDINATES: protein motif:HMM:NF025038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM46055.1"
                     /translation="MELITKKRLKPELLRYYPDLSKYKGKTTKNIKPVSQLVFQKRGF
                     EALDFGMTINKKGFNIYVAGDTGTGKTSNVKKHVEDIALKMGTPDDMLYVYNFKNPDA
                     PKLLYMKAGTGERFVKDMEDLLDYFIFQIPRQLESESFEIKKSEVVRWYQEESQKNYL
                     EIEKEALELSFLLKSTQNGLVINPIVEGNPIDKEKFDTLSDEEKEVIRENEEALQEKL
                     IAYFHRERGLEKEYKEKMTNLRQTMVRLIVSEPLRELKKKHSKNEHFDLFLEDLAEHI
                     VNNFDEFFIYRDIEEGKADPSKTVVPDFKEYKVNLLVNNKDLKGAPVIYETTPTFQSI
                     MGYFEFEEMHNSLFTDFTKMKPGALHKANGGFLIIQANDILKNYYAWTALKRAIRNRS
                     LKMGDIDIDFKYRVNTIPDPEPVTLDVKVILIGDDYIYHLLYNHDPDFRRIFRVKADF
                     DSTIDINADTTDSLIGFISRVVNEDCDLPFDLSAIRRLLHYSSRKSEDQKKYRIHASD
                     IVEIIVESVFWGKRKNKKMVDEELVRTAIESREKRHARFKEHYYESIKRGTILIDVKG
                     KKTGQINGLAVYSIGDFSFGIPSRITAKVFAGRKGIVNIERESRLSGAIHDKGAMILA
                     GYLGSIFAQDKQLSIIASIAFEQSYGGVDGDSASSTELYALLSALSEIPIKQHIAVTG
                     SINQMGDIQPIGGVNEKIEGFFEICKIKGLTGDQGVIIPIQNIENLNLSDEIVEAVRK
                     EKFHIYAIKNILEGIEILTGIPAGKKNSNGTFTAGSVFEKVDKKIWQLNEVK"
     gene            complement(4682..5668)
                     /locus_tag="PL056_03195"
     CDS             complement(4682..5668)
                     /locus_tag="PL056_03195"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HPM46056.1"
                     /translation="MAVVLEKTASNQIVLSGSIQNTSFFDILRDIALPKYSGMLKIKT
                     GKKEYRYYFKEGIIIYAENSIERMDQLILSTIKQAEIVSRETVVKCEKMKSKLMKSLL
                     EILIEEGHVSMLLYSKIISAAVRMNILEGFFARKGIYSFELRNSVREVHGVKSVDIRN
                     AQQLASMSDEKLKTVKKILLSMNCDILTNKDAAYFSVNKSFLQNFLVTEIDFIQYISK
                     AAEDFLSGGWTMRSRFATPVILKTGVLYLFRTFVLAGIFVFLYLATMTTTFETRKQHR
                     SVKDFYFFKISLMSSLMSFESGNETDTKDLIRSGMILEHEFDLSGTEKSDDK"
     gene            complement(5670..7442)
                     /locus_tag="PL056_03200"
     CDS             complement(5670..7442)
                     /locus_tag="PL056_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPM46057.1"
                     /translation="MRIFFSFTFFFFIFLNIQHICAENSEHSFIDGRRFTKDVKDALI
                     SGEESMLLEKTAMYLKEKEISHIPNCFECSLFLIRSTIDSSSAVQLVAADLAVKFSPD
                     LPEARLHFISRLIKFAPFKTARILSETREMIKAFFCFPPRDAFFYSVLNTILKACLVF
                     IFIYFLIIGLKYPSLIIHRYMHLAGHSKFYAVSLALVVALSSWILVQNEHNLFLVIMT
                     FLVFLTGVSVLREKIILYAVFIIALLAHGGIILTGASEDPSIDRKTAMNNLYAVYSPS
                     SSDIGKIELSAPGGQMARGFIHFYKDEHEKAVFHLKKELDIISDGKIKTSIYNILGLS
                     LAALGNYKEAVSYLKTAYDESGNIHIGYNLAKTMYEGGMTEDATNFEKQLLETAGAVT
                     LSYPHIEYPGMFRAWRYVSSGKTGDRFENWVRFFLFILSALFFYMMLLLVRMNYIKGI
                     SLSRCLECGIVMCSKCNAGGQHVCVVCKLMKADPEVFLKGEKEKYEVRRNRFFARHSV
                     YTSIFNFMCPGGGLIFSNKVLEGSAYLFITVFLSILIVNRNTGLVFNINTGDSGTMTF
                     ILTALIAVIYLISIVRGFFVSRGD"
     gene            7621..9831
                     /locus_tag="PL056_03205"
     CDS             7621..9831
                     /locus_tag="PL056_03205"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPM46058.1"
                     /translation="MREIIFKYPKTFLALISALVFMPLIGSYPVLGQWEPHYGRVAME
                     MMASGSWDWFLDPVYLGKHNFWSKPIFCFWMVIPFMKILGPTELALRLPFAINGILFV
                     LLVYYITEKLFKDRARSLAAGFITIFMPYTYLISRQFMWDIAFVTFLTGSIGFLFIGQ
                     RDGNKKLLRLSYLFMGLGMLTKGLLSVFFPVAIILIWMFVTLDYSKNTNEIFSDAWKF
                     VRSLRPFEGAGIFLAVSGWWYIYMGIRHGMPFFKEFFGEHHFGRLEGTINKPDGPFEF
                     YIWQLSIGTFPWIGFLIPGLYFSALKMKEKKEEGYVIISFFFMFLFFTLAATKFPHYI
                     FPVVPFMSIIVSVLFVELFRNEKMGKLYPVIAVVAALIVGIVGKDLGTGMNYADLIYI
                     ITTHKVQTWFGRVFDMLPYLAVFVPVMVVFVLVPLIAPLKKVLMKISVAGFVLTTVAW
                     SGYINFYWIPRMLDVFTPKLLVEKYFELKKDGDIIVDYDNWKNRSMYFYLGLDEHLNR
                     VDKVEQIQKLIEKHPENTVYITTKDKKVSELRSALLSKPGVPIKKIMDDAVDTYMEIE
                     MYSASMKDLNSAAMETWKENIVPEEELPKNLKKINSTIGQGSIEIVGYTINKDRFDPN
                     EEMILTVYYKILKKIEKSWKVFFHFDVYSGALPHSWKLDDFPQKGFYPTNKWEEGVIL
                     KDEFVTTIPKSHPGGGIKIYTGFYINKERMEIDNESFNDGQKRFILGTFNVNIK"
     gene            9828..10349
                     /locus_tag="PL056_03210"
     CDS             9828..10349
                     /locus_tag="PL056_03210"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HPM46059.1"
                     /translation="MTPSIFLFHTALEPVAIVWFKNNKQIKIKEIVISNPKFSAEQRV
                     AVKYPDSNFSTCGKVNQLAEKIENCCQGKNEEFDLEMFDFSQIPRFRKDVYFTLFKTK
                     RGETLSYGELALISGYPKAARAVGTAMKNNPFPFVIPCHRVIKSDGRLGGFFGAEEMK
                     RKLIELESGKIIY"
     gene            10439..11053
                     /locus_tag="PL056_03215"
     CDS             10439..11053
                     /locus_tag="PL056_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46060.1"
                     /translation="MKKLIFLIVFLFSGFSMYAESNPVDFNKVKFGMGGGLGFSTAIY
                     DDYGYYGYTIPTKVVMPVQIFKYFRFEPVFSLSVSKYNDNEHDESDTLTNFMGGGGFF
                     FTYPFDSAMIIAGARIEGGANIYKSEDEGETRKRHRGVFLAEPVIGADYFLNSHFSIG
                     CEITLSMVYMGETKYDDVESDDYEGETAFMIRTGGTLFFRFYFF"
     gene            complement(11072..12013)
                     /locus_tag="PL056_03220"
     CDS             complement(11072..12013)
                     /locus_tag="PL056_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HPM46061.1"
                     /translation="MTNICLICGKPHINGTEYHPICSKKFFGSDAAPVLEYKLDEINK
                     IAEETVLKSITLTGVQPKLSLHLSKSVKEKGRKLTIVGLWGSYILKPPSPTYPFLPEN
                     EHLTMHLAEHCGIKTVPHALIKFQSGELAYITKRIDRSKSGKLHMEDMCQLQERLTED
                     KYRGSVEQIGKIIERHSSNSGYDLQRLFEMVVFSFLTGNADMHLKNYSLFYRLPDFRE
                     LAPAYDLLNTAIVIPSDKEESALTINGKKSKLKKSDFDHLAKNLQLIQKVKESIIDRI
                     ISSLSQSSKIIQNSFLNEEMKEKYMNTISSKINQISL"
     gene            complement(12010..12336)
                     /locus_tag="PL056_03225"
     CDS             complement(12010..12336)
                     /locus_tag="PL056_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007897912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA N-terminal domain-containing protein"
                     /protein_id="HPM46062.1"
                     /translation="MSRKTGFVFMHDEFAGIIEETETGYRFTYDPDYVSKSGSIAISC
                     TIPLRKEPYESPVMFPFFDGLIPEGWLLDIAWKSWKINSKDRMELLLACCSDVIGAVS
                     VRREKQ"
     gene            complement(12333..12548)
                     /locus_tag="PL056_03230"
     CDS             complement(12333..12548)
                     /locus_tag="PL056_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004297664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPM46063.1"
                     /translation="MSSEKLRNFVKKRRKMLGMTQLELSERSGTGLRFIRELEQGKAT
                     LRLDKINQVLALFGHEMAPVQIERGDK"
     gene            12773..13102
                     /locus_tag="PL056_03235"
     CDS             12773..13102
                     /locus_tag="PL056_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGGC domain-containing protein"
                     /protein_id="HPM46064.1"
                     /translation="MKVGIIRCQQTEDMCPGNTDFKVAKLGKLAFEETGPVEIIGFVS
                     CGGCPGKKAVSRAKMMVERGAEAIVLASCISRGNPIGFPCSHFKNMKESIEKAIGPDI
                     KIIDWTH"
     gene            13376..>13863
                     /locus_tag="PL056_03240"
     CDS             13376..>13863
                     /locus_tag="PL056_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family antitoxin"
                     /protein_id="HPM46065.1"
                     /translation="MDDIAHPKRENQRVMLVEVSGDVYFVPYVLEKDGNIFLKTAFIN
                     RKYRMKNSKEEEMNNKKKNDGTFFVKEPDFTYNSEEIEEKELHDLYESGAFTAVTDER
                     KKELAEDAKKTLSEMRAIKSITLRINSDILEMIRRKADAERIPYQTLMQLVLSQFAQG
                     KI"
     assembly_gap    13864..13963
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13964..14518
                     /locus_tag="PL056_03245"
     CDS             <13964..14518
                     /locus_tag="PL056_03245"
                     /inference="COORDINATES: protein motif:HMM:NF016144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HPM46066.1"
                     /translation="LSEMTADELGRLFPISLSDHDPEWKGLFESEKRLIEELTGVENI
                     VRITHVGSTSVSGIKAKPIIDILLEVVEFFDKTAFIKRFVDAGYNYIPQPKNSAPHIM
                     LVKGYTDSGFAGQVFHIHVRFSGEWEEIKFCEYLRQHPEIAKEYEKLKIELAQIHKYN
                     RENYTNAKTDFITKINEIIRSEEK"
     gene            14515..15171
                     /locus_tag="PL056_03250"
     CDS             14515..15171
                     /locus_tag="PL056_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HPM46067.1"
                     /translation="MKYTNILFDLDGTLTDPKEGIINSVKFALAKFGVNESEVGDLMK
                     FIGPPLHVSFRDFCGFNEENSELAVQYFREYFTDKGINENRIFPGVVELLESLKSRNL
                     KIILATSKPTVHANTVLNNFEIAKYFQYVKGSNLNGTESGKTEIISHIIDKFKLEKDK
                     TVMIGDRFYDIIGAKENGIDSIGVTFGYGGETELKEAGATYICRDIKDINNLLLRGTQ
                     "
     gene            15168..15551
                     /gene="mscL"
                     /locus_tag="PL056_03255"
     CDS             15168..15551
                     /gene="mscL"
                     /locus_tag="PL056_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HPM46068.1"
                     /translation="MKIINEFKTFAMRGNVIDMAVGIIIGAAFGKIVSSIVGDVIMPV
                     IGLFVGGLNFTTLQFTVGESVIKYGNFIQVTFDFTIVAFAIFMLIKGLNSLKKKEEAA
                     PAPAEPPAPPADIVLLTEIRDLLKK"
     gene            complement(15560..16045)
                     /locus_tag="PL056_03260"
     CDS             complement(15560..16045)
                     /locus_tag="PL056_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46069.1"
                     /translation="MKNSLKLAVIIIAVLSVMPLAAQKMGKGHSGKKGEHMMMMHDKM
                     LEDAGVPDVKRAEIRKKGHEIHKEMMDINYSIGELRIKMKDEYEKAKPDINSLKSLNN
                     QIAELRKKQFILMGNFKIDTFSSLTPDQRKKMLELMKDKRKKFMGRMGGRGPDPMDDD
                     E"
     gene            complement(16059..16331)
                     /locus_tag="PL056_03265"
     CDS             complement(16059..16331)
                     /locus_tag="PL056_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46070.1"
                     /translation="MNNENMKWEKMLNDVMVEGKKIKKRNTLVKTFSGISFVILLAAS
                     VFVTTYKDNNINRDDLMATDLSGNEIEVALVAADAFFDEDLEMIIY"
     gene            complement(16333..>16897)
                     /locus_tag="PL056_03270"
     CDS             complement(16333..>16897)
                     /locus_tag="PL056_03270"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPM46071.1"
                     /translation="FAVIISGRPGGGTMFDRTDYDNYFKELFWNKRFFVQSVIRKYVK
                     NPDRVDDIVQQTFLKIYLNIKKVHNSANPDGYIHSVAVNETMNFFKKSKEMPEVTIDD
                     VLEHILPDKNVNIEMDMENSSVIETFGNTLAALPAKRRAVVSLRIFEEKSFSDISKML
                     GMSEVSARNLFSIAMKSIRRKFIKQGV"
BASE COUNT         4879 a         3232 c         3377 g         5309 t
ORIGIN      
        1 acagggttcg gaattttaag cgatgtaaag atagcacatg aaaatcttga agagcttcag
       61 ataaatctta tcagaagtca cgctattgcc gtgggaaccc cttatgacaa agaaacagtc
      121 cgggccataa tggttctgag ggcaaatgtt cttgcaaagg gctacagcgg aattacactt
      181 gaaacttttg acaaactcct tgaaataatt aataaaaata ttgttccttt cattccctgc
      241 caggggagtg tcggggcgag cggcgatctt gcaccattgg ctcatcttgc actggttctt
      301 gtgggtgaag gatttgtgat aaatgagcat ggcgaaaagg tggatactgc tgtaatattt
      361 aaagaaaaag ggataacgcc cgttgttctg aaagcaaagg aagggcttgc tctgatcaat
      421 ggaactcagg caatgaccgc aatagggtgt cttacggtga gcagagtcag aaatctgctg
      481 aaacttgctg atattgcagt tacttcaact cttgaaggga taatggggac agacagagca
      541 tttgatagaa aagtgaatgt tgtgagagct caccgcggac agatggaagt ttcagaaaat
      601 gtgcttagaa taattaagga ttctgaaatc agggatagtc atacatgctg ttcaaaggtt
      661 caggatgcct attctctccg ctgtgctccg caggttcacg gagcggtcag agatgcatta
      721 agacatgccg aatctatcat aactactgaa attaattcat caactgacaa tccgcttatt
      781 tttcctgaag aaaaagagac catttccgcc ggcaacttcc acggtgaacc gataggactt
      841 gtgatggatt atctcacagc ggcagtttca gagatcggaa gcatttcgga aagaaggatc
      901 gagcagatga taaaccctgt cttttcgcag gccccggcat ttctggtaaa agagaaaggg
      961 cttaacagcg ggtttatgat cgcccatgtc acagcggcaa gcattgcaag tgaaaataaa
     1021 actctctgct tccctgcgac agttgattcg attcccacct cagcaggcaa ggaagatcat
     1081 gtcagcatgg ggacaacagc cgcaagaaaa tgtgctcaag ttgttgataa cacagtgaaa
     1141 attatatgca tggagatttt taccgcaatg caggcgatag atttcagaaa accgcttatc
     1201 cctggagcag gagtaaaagc cgcatatgaa cttataagga aaacagtgcc tttcatggaa
     1261 aaagaccggt tcatgtatcc catgttcgaa aatgtcctga aaaacgaata tctcatagtc
     1321 gaagaagtcg aaaaagttac tggggttctt aactgaagat cttaaatatt acggtttgtg
     1381 aaaaacgatc tttccaccgc ttttctcaat taggtcgtct ttgaatttct cgactgtccc
     1441 gataggtata aatatttcca gcaaaatgcc tttttcgttg taactatttg aaataatttc
     1501 aattcctgaa aagtggctga tatggttcat caccagtcct gtgagatcga acgatgcttc
     1561 cactgttacg gttttcttta tctccactgt tgcaattgcg gctttttcaa gtacttctct
     1621 tgctgatccg ccgtatgccc ttgtgagtcc tccagttccg agttttgttc ctccgtaata
     1681 tctgacaatt gtgacaagca cattgaatag ctcttttctc aatatttcat tgtatatagg
     1741 cagtcccgca gttcccgaag gttctccgtc atcactatat ctgaaaagtt ttatttcacc
     1801 ttctccgaga atgtaaccat aacagacatg tgttgaatca tagtgctctt tcttctgtgt
     1861 tttcaggatg ttatctatat catctttcgt tttgacatgg aaacagtatg aaatgaaacg
     1921 acttcccttt tcctttattt cgactggtcc tgcctgctca aagactgttt tgtagcgttc
     1981 aatcatatct ggtcgaagat cttctcaact gccgatctta tgtgaggagt aaaaaatctg
     2041 taaagaagag gtatctcgac aacgatactt acacaaccgt tgtttaaaac aagtgttccc
     2101 ctgacggtac gacccttaaa ttcgatcttt tttgaatttt tatcggggat cacctcaatt
     2161 ttttcaactc cgatcttttt ttcaagtatg tgcttaaggt tgttttcaag ccactcccag
     2221 atctctttat cttgcttaga gctttgaaaa ttcatttcga ttttaggcat ctttactatt
     2281 ttacctcgtt gagctgccag atctttttat caactttctc aaatactgag cccgcagtaa
     2341 atgtaccgtt gctgttcttt tttcctgctg gaattcctgt aaggatttca attccttcaa
     2401 gaatgttctt gatagcataa atatggaatt tctcttttct gacagcttcg acaatttcat
     2461 cagacagatt aagattttca atgttctgaa tagggataat aactccctga tctcccgtga
     2521 gcccttttat tttgcatatc tcgaaaaacc cttctatctt ttcgtttact ccgcctatcg
     2581 gctgaatgtc tcccatctgg ttgatggagc cggttactgc aatgtgttgt tttatcggga
     2641 tctcagacaa tgcggaaagg agcgcataaa gctcggtgga gcttgcactg tcaccgtcaa
     2701 ctccgccata actctgctca aaagcgattg atgcaatgat tgaaagctgt ttgtcctgag
     2761 caaagatgct gccaagataa cccgccaata tcattgctcc tttgtcatga atagctccgg
     2821 acaggcgaga ttctctttca atgttgacga tcccttttct tcctgcaaaa acctttgctg
     2881 ttattcttga ggggatgccg aaactgaagt caccgatcga atatactgca agtccgttta
     2941 tctgaccggt cttttttcct ttgacatcga tgagaatggt gcctcttttt attgattcat
     3001 agtagtgttc cttgaaccgt gcatgtcgtt tctccctgct ctcgatcgca gttctgacaa
     3061 gttcctcgtc caccatcttt ttattctttc ttttacccca gaaaactgat tctacaataa
     3121 tttcaactat atcgcttgca tgtatcctgt attttttctg gtcttccgat tttcttgaac
     3181 tgtaatggag aagccgtctg atagcggaaa ggtcgaaagg aagatcgcaa tcttcattga
     3241 caacccgtga aatgaaaccg atcaacgaat ctgttgtgtc agcattaata tcaatagtcg
     3301 agtcaaagtc ggcttttacc ctgaatatcc ttctgaaatc aggatcatgg ttgtagagaa
     3361 gatggtagat ataatcatcg ccgatcagga tgactttaac atcaagtgtg acaggttccg
     3421 ggtcgggaat ggtattgacc ctgtacttga aatctatgtc gatatctccc attttgagag
     3481 agcggttcct tatcgccctt ttgagagctg tccatgcata atagttttta aggatgtcgt
     3541 tcgcctggat gatcaggaat ccgccgttcg ccttgtgaag tgcgcccggc ttcatttttg
     3601 tgaaatctgt gaaaagggag ttgtgcatct cttcaaattc gaaataaccc attatgctct
     3661 ggaatgtcgg agttgtttca taaattaccg gagccccttt cagatctttg ttgttcacca
     3721 gcagattaac tttgtattcc ttaaaatcgg gaacaactgt ctttgacgga tcggctttgc
     3781 cttcctcaat gtccctgtaa ataaaaaact catcgaaatt attgacgata tgttctgcaa
     3841 gatcttcaag gaaaagatca aaatgctcgt ttttagagtg ttttttcttg agctctctca
     3901 aaggttccga aacgatcagc cgtaccatgg tctgacggag attggtcatt ttttccttat
     3961 attctttttc aagacctctt tctctatgga agtaagctat cagtttttcc tgaagtgcct
     4021 cttcgttttc tctgatgacc tctttttctt catcgctaag agtatcaaac ttctccttgt
     4081 caataggatt cccttcaact atcgggttta ttaccagtcc gttttgtgtc gacttaagaa
     4141 ggaaactcag ttcaagagct tctttttcta tttcaagata gttcttctga ctttcttcct
     4201 gataccacct tacaacttca ctctttttta tctcaaaact ttcactttca agctgtctcg
     4261 ggatctgaaa aataaaataa tcaagaaggt cttccatatc cttgacaaat ctttcaccgg
     4321 tgccggcttt catgtagaga agtttaggcg catcagggtt tttaaagtta taaacataga
     4381 gcatatcatc cggagtgccc attttcagag cgatatcctc aacatgtttt tttacatttg
     4441 atgttttacc ggttccggta tcgccggcta catatatgtt gaaccccttt ttgtttatag
     4501 tcattccgaa gtccagtgcc tcaaagcccc ttttctgaaa aacgagctgg gagacgggct
     4561 tgatgttttt tgttgttttt cccttatatt ttgagagatc agggtagtat ctcaacaatt
     4621 cgggtttaag tcttttctta gttatcagtt ccatatttcc ctccgtttcg gaataaatcg
     4681 tttatttgtc atcacttttt tcagtaccgg acagatcaaa ttcatgctcc aagatcattc
     4741 ctgacctgat cagatctttg gtgtctgttt catttccgct ctcaaatgac atgagagaag
     4801 acataagcga tattttaaaa aaataaaaat ctttgacact tctgtgctgt tttctggttt
     4861 caaatgttgt tgtcattgtt gcaagataaa ggaaaacaaa tattcctgct aaaacaaatg
     4921 ttctgaaaag atacagcact ccggttttta agatcacagg tgttgcaaac ctgctgcgca
     4981 ttgtccatcc gccgcttaaa aaatcctcag ccgcttttga gatgtattgt atgaaatcta
     5041 tctctgtaac aagaaagttc tgtagaaatg acttgttaac tgaaaaataa gccgcatctt
     5101 tgtttgtcag aatgtcacag ttcattgaaa gaaggatctt tttgacggtt ttcagttttt
     5161 catccgacat tgaagcaagc tgctgagcgt ttcttatgtc aacagatttg acaccgtgaa
     5221 cctctctgac actgtttctg agttcaaaag aataaattcc ttttctggca aaaaaaccct
     5281 caagtatgtt cattctcaca gccgcactga tgatctttga atataaaagc attgagacat
     5341 gaccttcttc aatgagtatt tcaagaagac ttttcatcag tttcgatttc attttttcac
     5401 attttacgac agtctctctt gaaacgatct cagcttgttt tatcgtcgaa aggataagct
     5461 ggtccattct ttcaattgaa ttttcagcat aaatgatgat tccttccttg aaataatagc
     5521 ggtattcttt tttgcctgtt tttattttaa gcattcctga atacttggga agagcaatgt
     5581 cccttaaaat gtcaaaaaaa gatgtatttt gtatagaacc gctcaatact atctggtttg
     5641 atgccgtttt ttccagaaca acagccaaat caatcccccc ttgatacgaa gaatccgcgc
     5701 acgattgata tgagatatat tacagctatc agtgctgtta aaataaatgt cattgtgccg
     5761 gaatcccctg tatttatgtt gaaaacaagc ccggtgttcc tgttaacgat cagtattgaa
     5821 agaaaaaccg ttataaaaag atacgcagag ccctcaagaa ctttattcga aaatattaga
     5881 cccccgcccg gacacatgaa attaaatatg gatgtgtaga cagagtgtct tgcaaagaac
     5941 ctgtttctcc tgacctcata tttttccttt tcacctttta agaaaacttc aggatcggct
     6001 ttcatcagtt tgcagacaac gcacacatgc tggccgcctg cattgcattt tgaacacatg
     6061 acgataccgc attccagaca tcttgagaga gatattcctt taatatagtt cattcgtaca
     6121 agcagaagca tcatatagaa gaacagggcg cttaaaataa aaagaaaaaa tcttacccag
     6181 ttttcaaatc tgtctcctgt ctttccgctt gaaacatatc tccatgccct gaacattccc
     6241 ggatattcaa tatgaggata tgaaagagtt acggcacctg ccgtttcaag gagttgtttt
     6301 tcaaaatttg tcgcatcttc ggtcattccg ccttcgtaca ttgtttttgc aaggttatag
     6361 ccgatgtgga tattgccgga ttcatcatag gcggtcttca gatagctgac tgcctcttta
     6421 tagtttccaa gtgccgcgag ggaaagcccg aggatattat aaatagatgt ttttattttt
     6481 ccgtctgata tgatgtcgag ttcttttttc aggtgaaaca cagctttttc atgctcatct
     6541 ttataaaaat gaatgaaccc tctagccatc tgaccgccgg gagctgaaag ttctattttc
     6601 ccgatgtccg aggatgacgg cgagtaaacg gcatacagat tgttcatggc tgtctttctg
     6661 tctatgctcg gatcttctga agctcccgtg agaattattc cgccatgggc gagaagcgca
     6721 ataatgaaca ctgcatacag tattattttc tccctcagaa ccgaaactcc tgtcagaaat
     6781 accagaaatg tcataatgac aaggaaaaga ttgtgttcat tctgaacaag tatccacgaa
     6841 gagagcgcta caactaaagc aagtgacacg gcatagaatt ttgaatgacc tgcaagatgc
     6901 atataccggt ggatgatcag agaaggatat ttcaatccga tgatgagaaa gtatatgaag
     6961 atgaaaacta gacatgcttt aagtatggtg tttaaaacgg aatagaaaaa agcgtctctt
     7021 ggaggaaaac agaaaaaagc tttgatcatt tctcttgttt cactcaagat ccttgccgtt
     7081 ttaaaaggtg cgaactttat aagccgtgat atgaaatgaa ggcgtgcttc aggaagatcg
     7141 ggggagaatt ttacagcgag gtcagcagcg acaagttgaa ccgcagaaga ggaatcaatt
     7201 gtagatcgta taagaaaaag agagcattca aaacagttgg gaatgtggga tatctctttt
     7261 tccttgagat acatggctgt tttttcaaga agcatgcttt cttcaccgct tatcagggca
     7321 tctttgacat ctttggtaaa ccttctgccg tctatgaatg aatgttctga attttccgca
     7381 caaatatgct gtatgttcaa aaatatgaaa aagaaaaaag taaaactaaa gaaaattctc
     7441 atttatcagc atccttaaca atcaaagaga tatatcatat tgagaataag ttatcaaatt
     7501 tttgtttttt tgacatggat ttgctttgta tctataatca cgaagttttt tttagatcat
     7561 tttgggaaat gcaatgaata atgaaaaagt tagtgcagaa tttggcggag attcagtaaa
     7621 atgagagaaa ttatatttaa atatccaaag acttttctgg ctttgatatc ggcattggta
     7681 tttatgcctc tgataggcag ttatcctgtt ttaggtcagt gggaaccgca ttacggcagg
     7741 gttgcgatgg agatgatggc aagcggttca tgggactggt tccttgatcc tgtctatctc
     7801 ggaaagcaca atttctggtc aaaacctata ttctgtttct ggatggttat cccgttcatg
     7861 aaaattctgg gaccgaccga gctggcgctg cgacttccct ttgccataaa cgggatttta
     7921 ttcgttctgc tggtatatta catcacagaa aagcttttca aggacagggc gagatcgctt
     7981 gcggcgggct tcataacaat attcatgcct tacacatatc ttataagccg gcagtttatg
     8041 tgggacattg cttttgtgac attcctgacc ggctcgatag ggtttctgtt cataggtcag
     8101 cgtgacggga acaagaaact tttacggctc tcatatcttt tcatgggtct tggaatgctg
     8161 acgaaaggac ttttgtctgt tttttttcct gttgcgataa ttctgatatg gatgtttgta
     8221 acgcttgatt attctaaaaa tacaaatgaa atattttcag atgcatggaa atttgtcaga
     8281 tctctccgcc cgtttgaagg agccggaata ttcctcgctg taagcggctg gtggtatatt
     8341 tacatgggga tcagacacgg aatgcctttt ttcaaggagt ttttcggaga gcatcatttc
     8401 ggccggcttg aagggaccat caacaaaccc gacggtcctt ttgagtttta tatctggcag
     8461 ctcagcatag ggacttttcc atggatcggt tttttaattc cggggcttta tttcagcgct
     8521 ttgaagatga aggaaaagaa agaggaagga tatgtcatca tctcattttt cttcatgttc
     8581 ctgtttttca cccttgctgc gacaaagttc cctcactata tttttccagt cgttccgttc
     8641 atgtcgatca tagtttcagt cctgttcgtt gagcttttca gaaacgaaaa gatgggcaaa
     8701 ctttatccgg taattgccgt agttgcggcg cttatagtag gtatcgtcgg aaaggatctc
     8761 ggaacgggaa tgaactatgc cgatcttatc tatatcatta cgacacataa agttcagaca
     8821 tggttcggca gagtttttga catgcttccg tatcttgctg tttttgttcc ggtcatggtt
     8881 gtttttgtgc ttgttccctt aatagctcct ttaaaaaaag tgctgatgaa aatatctgtt
     8941 gccggttttg ttttaacaac tgtcgcatgg tcgggatata ttaattttta ctggattccc
     9001 agaatgcttg atgtgttcac acccaagctt cttgttgaga aatattttga actgaaaaaa
     9061 gatggcgaca taatagttga ctatgacaac tggaagaacc ggtcaatgta tttttatctg
     9121 gggcttgatg agcatttgaa cagggttgat aaagttgaac aaattcaaaa gcttatagaa
     9181 aaacaccctg aaaacactgt ctatatcaca acaaaggaca aaaaggtgtc tgagctcagg
     9241 tcggcactcc tttcaaaacc cggtgttcct ataaagaaga tcatggatga tgccgttgac
     9301 acttatatgg aaatagaaat gtattccgct tcaatgaaag atctcaacag tgcggccatg
     9361 gagacatgga aggaaaacat tgtgcccgaa gaggaacttc ctaaaaatct caaaaaaata
     9421 aattccacga tcggtcaggg ttcaatagaa atagttggat ataccataaa taaagaccgg
     9481 ttcgatccaa atgaggaaat gatccttacg gtttattata agatattgaa aaaaatagag
     9541 aaaagctgga aagtgttttt tcattttgat gtttacagcg gtgctctccc gcacagttgg
     9601 aagcttgatg atttcccgca gaaagggttt tatccaacaa ataaatggga ggagggagtc
     9661 attcttaagg acgaatttgt cacaacaatt cccaaaagtc atccgggagg cggaatcaag
     9721 atatataccg gtttttacat caataaggaa agaatggaga tcgacaacga atctttcaat
     9781 gacggacaga aacgattcat cctcggaact ttcaatgtca acatcaaatg accccttcga
     9841 tctttttatt tcacaccgct cttgagccgg ttgcaattgt ctggtttaaa aacaacaaac
     9901 agataaagat aaaagagatt gtgatttcaa atccgaaatt ctcggctgaa cagagggttg
     9961 ccgtaaaata tcctgattca aatttctcaa cttgtggaaa agtgaatcaa cttgctgaaa
    10021 agattgagaa ttgctgtcaa ggcaaaaacg aggaatttga tcttgaaatg tttgactttt
    10081 ctcaaattcc cagatttaga aaagatgtct attttacact tttcaagaca aagcggggcg
    10141 aaacgctgag ttacggcgaa cttgcactta tctccggtta tccaaaagcc gcaagagcag
    10201 tcggaaccgc aatgaaaaac aacccgttcc cgtttgtgat tccctgccac cgtgtcatca
    10261 aatccgatgg tcggctcggc ggattcttcg gagccgaaga aatgaaaaga aagctcatcg
    10321 aactcgaatc tggcaaaatt atttattaaa aaagtagatt ttctaatatc cattaatata
    10381 ctattttaaa cagtgtgttt tgttaagggg tgttttttat ttttatctgg aggtcgtgat
    10441 gaaaaagtta atttttttga tcgttttctt attttccggt ttttcaatgt atgctgaatc
    10501 caatccggtg gattttaaca aagtcaaatt cggaatgggt ggaggactgg ggttttccac
    10561 tgctatttac gacgattacg gttattacgg ctacactatt cctacaaaag tcgtcatgcc
    10621 tgtacaaatc tttaaatatt tccgttttga gccggttttc agtctttcag taagtaaata
    10681 caatgacaac gagcatgatg aatcggatac tctaacaaat ttcatgggtg gtggcggatt
    10741 ttttttcaca tatccatttg attcagcgat gatcattgcg ggagcacgaa ttgaaggcgg
    10801 agccaatatc tacaaatcag aagatgaagg tgaaacaaga aaaagacaca gaggcgtttt
    10861 tcttgcggaa ccggttattg gtgcggatta ctttttaaac agccacttca gtatcggctg
    10921 cgaaatcact ttaagcatgg tatatatggg agaaaccaaa tatgacgatg tggaaagtga
    10981 tgattatgaa ggagaaacgg catttatgat cagaaccggc ggcacgctgt ttttccgttt
    11041 ttatttcttt taaagctcct ttcaaaaaag atcaaagcga aatctgatta atttttgagc
    11101 ttattgtgtt catgtatttt tctttcattt cttcatttag aaatgagttt tgaattattt
    11161 ttgaactttg agaaagtgaa ctaattatcc tgtcgatgat ggattcttta accttttgaa
    11221 ttaattgcag gtttttggca aggtgatcga agtcagattt tttaagcttt gatttttttc
    11281 cgtttattgt gagtgctgat tcttctttgt cagatggaat aactattgcg gtattaagaa
    11341 gatcatatgc gggtgcaagc tctcgaaaat cgggcaaacg ataaaaaagt gaatagttct
    11401 ttaaatgcat gtcggcgttc cctgtcagaa acgaaaaaac aaccatttca aaaagccgct
    11461 gaagatcata tccggagttt gaagagtgtc tttcaatgat ttttccgatc tgttcaactg
    11521 atccccggta tttatcttcg gtcagccttt cctgaagctg gcacatatct tccatgtgaa
    11581 gctttcccga ttttgatctg tcgatccttt ttgtgatata tgcaagttca ccgctttgaa
    11641 attttatcaa ggcgtgggga acggttttaa ttccgcagtg ctctgcaagg tgcattgtaa
    11701 gatgttcatt ttcaggaaga aatggatagg tcggtgatgg cggttttaaa atatatgatc
    11761 cccaaagtcc gacaatcgtg agttttctcc ctttttcttt gaccgatttt gataaatgaa
    11821 gtgaaagttt cggctgaact ccggtaagag taatgctttt taatacagtt tcttcagcga
    11881 tcttgttgat ctcatcaagt ttatattcaa gaactggagc agcatcagag ccgaaaaact
    11941 ttttagaaca gatcgggtga tattcagtcc cgtttatatg aggtttccca cagataagac
    12001 agatatttgt cattgttttt ccctccgtac tgaaactgcg ccgataacat cactgcaaca
    12061 ggcaagaaga agctccattc tgtcttttga attgatcttc cagcttttcc atgcaatatc
    12121 gaggagccat ccttccggaa taagtccgtc aaaaaacgga aacataaccg gtgattcata
    12181 tggctctttt cttaaaggaa ttgtgcaact tatggcaatt gaaccgcttt tggaaacata
    12241 atcaggatca taagtgaatc tgtaccccgt ttcggtttct tcaataattc ccgcaaactc
    12301 atcatgcatg aaaacaaatc cggtttttcg gctcatttgt cacctctttc gatttgaaca
    12361 ggagccattt cgtgcccgaa cagcgcaaga acctgattga tcttgtcaag ccggagagta
    12421 gcttttccct gctccagttc acggatgaac cgcagacccg ttccactccg ttcagaaagc
    12481 tcaagctggg tcataccgag catttttctt ctttttttaa caaaatttct aagtttttct
    12541 gaactcatat tatacctcat cgggtgtaaa aaacatcaaa cattatgatt ttatacctta
    12601 tcgggtgcaa ttgtcaagtt gttttgtgct tttatacccg aaagggattg aattcgattt
    12661 tttaatcaaa tcagaaatga taataaaatt tgcgattttg tttctaaaac atcataatat
    12721 tggcggattt ggaaagtaga tttaaaggga atcagttttt ttggaggaga caatgaaagt
    12781 tggaattatc cgttgtcagc agactgaaga tatgtgtccg ggcaacaccg attttaaagt
    12841 tgcaaagctt ggaaaacttg cgtttgaaga gactggtccg gttgaaatca tcggttttgt
    12901 ctcttgtggc ggatgtccgg gcaaaaaggc agtctcaaga gcaaaaatga tggtggaaag
    12961 gggtgcagag gcaatcgttt tagcttcatg tataagcaga ggaaatccga taggttttcc
    13021 atgttcgcac ttcaaaaaca tgaaagaaag catagaaaaa gccatcggtc ctgacataaa
    13081 gatcatagat tggacacatt agtattcggg agatcgtttg aaatacacaa acattctatt
    13141 tgatctgaac tgaccctgat taactctcaa tacattttgt aaaaccggac attttaactt
    13201 tgctgacatt tttaaaaagt atgttgactt gtattttata agatgtaaaa taagaatgtt
    13261 ttagattctt tataggtgtc taatgagatt tgatattgaa aaagatttaa gattgcaaaa
    13321 cgagcgtggc ataagcttcg aaatgataat tgaagcgatt tctgagggaa aggttttgga
    13381 tgatatcgct catcccaaaa gagaaaatca gagagttatg ctggttgaag tttcaggaga
    13441 tgtttatttt gtgccgtatg ttttagaaaa agacgggaac atttttctta aaacggcttt
    13501 tattaacaga aagtacagaa tgaaaaattc aaaggaggaa gagatgaata ataaaaagaa
    13561 gaacgatggc acattttttg ttaaagaacc tgattttaca tataattctg aagaaatcga
    13621 agaaaaagag cttcatgatc tttatgagtc cggtgcgttc actgcggtga cagacgaaag
    13681 aaaaaaagaa ctcgctgaag atgctaaaaa aacgcttagt gaaatgagag cgataaagag
    13741 cataactcta agaattaaca gcgatattct tgaaatgatc cgcagaaaag ccgatgctga
    13801 aagaattccg tatcagacac ttatgcagct tgttttaagc cagttcgccc agggaaaaat
    13861 aaannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnctttcag aaatgacggc
    13981 tgatgaactc gggcggctgt ttcctatatc tctgtcagat catgatccgg agtggaaagg
    14041 attatttgaa tctgaaaaga ggttgataga agagctgaca ggagttgaaa acattgtgcg
    14101 gataactcat gtaggaagca cttcagtttc tggaataaaa gcaaaaccta tcattgatat
    14161 tctgcttgaa gtggttgaat tctttgataa aacagcattt ataaaaagat ttgtcgatgc
    14221 cggatacaat tatattccac aacctaaaaa ttctgctccc cacattatgc ttgtaaaagg
    14281 ttataccgat tcaggatttg ccggacaggt gtttcatatt catgtcagat tcagtggtga
    14341 atgggaagag atcaaattct gtgaatatct gagacaacat cctgaaattg caaaagaata
    14401 tgaaaaactc aaaattgagc ttgcacaaat ccataaatat aaccgcgaaa attacacaaa
    14461 cgcaaaaaca gatttcatta caaaaattaa cgaaataatc agaagcgaag agaaatgaaa
    14521 tacacaaaca ttctatttga tcttgacgga acattgacag acccgaaaga gggtattata
    14581 aattctgtta aatttgcttt ggcaaaattc ggagtgaacg aaagcgaggt tggtgatctg
    14641 atgaagttca tcggaccgcc gcttcatgtc tctttccggg atttctgcgg ttttaatgaa
    14701 gaaaattcag aacttgcagt gcagtatttc cgtgaatatt ttactgataa aggtatcaat
    14761 gaaaatagaa tatttccggg agttgtcgag cttcttgaat cacttaaaag ccggaatttg
    14821 aagataattc ttgcaacttc aaaaccgaca gttcacgcaa acacagttct caataatttt
    14881 gaaatagcca agtatttcca atatgttaaa gggagcaacc ttaacggcac agaatcagga
    14941 aaaacggaaa taatcagcca cataatcgac aaattcaaac tcgaaaaaga taaaacagtc
    15001 atgatcggcg accggtttta tgacatcatc ggcgcaaaag agaacggcat cgactccatc
    15061 ggcgtcacat tcggatacgg cggcgaaaca gaactcaaag aggcaggcgc gacttatatt
    15121 tgccgcgata taaaagatat taacaactta cttttgagag gaacacaatg aaaatcatta
    15181 acgaattcaa gactttcgcg atgaggggaa atgtcattga catggctgtg ggtatcatta
    15241 tcggagccgc tttcggcaag atcgtaagtt ctattgtcgg tgatgtgatc atgccggtga
    15301 tcggactttt tgtagggggc ttgaacttta caactttgca gtttacagtg ggtgaatcgg
    15361 ttatcaaata cggtaatttc attcaggtta cattcgattt caccatcgtt gcttttgcaa
    15421 ttttcatgct gataaaggga cttaattccc ttaaaaagaa ggaagaagcg gctcctgctc
    15481 ctgctgaacc gcctgctcct ccagctgata ttgttctgct taccgaaata agagatcttc
    15541 tgaaaaaata agagattttt tattcgtcgt catccatcgg gtcgggacct ctgccaccca
    15601 ttctgcccat gaatttcttt cttttatctt tcatgagctc cagcatcttt tttctctggt
    15661 cgggagtaag tgaactgaaa gtatcaattt tgaaatttcc catcagaata aactgtttct
    15721 tcctgagttc agcaatttga ttgttcaatg attttaaaga gttaatgtcc ggttttgctt
    15781 tttcatactc gtctttcatt tttattctca gttcgccgat actatagttg atgtccatca
    15841 tttccttgtg gatttcgtgc ccttttttac ggatttctgc cctttttaca tcgggaactc
    15901 ctgcatcctc gagcattttg tcatgcatca tcatcatgtg ctcacccttt tttccactgt
    15961 gtccttttcc cattttctgt gcggcaagcg gcataactga taaaactgca attattatca
    16021 ccgcgagttt aagtgaattt ttcatcttct cctccatttt aataaattat catttccaga
    16081 tcttcatcaa aaaaagcatc ggctgcaacg agagcaacct ctatttcgtt accgctcaaa
    16141 tctgtggcca tcagatcatc gcggttaata ttgttgtctt tataggtagt tacaaacact
    16201 gaagctgcaa gcagaataac aaaactgatt ccgctgaatg tttttactaa tgtgttcctt
    16261 tttttgatct ttttcccttc aaccatgaca tcgttgagca ttttttccca tttcatgttt
    16321 tcattattca ttttacaccc cctgtttaat aaattttctt ctaatacttt tcatagcaat
    16381 tgaaaaaaga ttccttgctg aaacctcaga cattccgagc atttttgaaa tatctgaaaa
    16441 gcttttctcc tcaaagatcc gaagagagac aactgctctt ctttttgccg gaagtgcagc
    16501 gagagtattt ccaaaagttt cgattacaga gctgttttcc atatccattt ctatgttgac
    16561 atttttatca gggagaatgt gttcaaggac atcatcaatt gtaacttcag gcatttcctt
    16621 ggatttcttg aagaaattca ttgtctcatt tactgcgacg gaatgaatgt atccgtccgg
    16681 attcgctgaa ttgtgaactt tttttatgtt taggtatatc ttaagaaaag tctgctggac
    16741 aatgtcgtca actctgtcgg gatttttcac atattttctg atcactgact gaacaaagaa
    16801 ccttttgttc cagaaaagtt ccttgaaata attgtcgtaa tctgttctgt caaacattgt
    16861 ccctcctccg ggtcgtccgg atattataac cgcaaaa
//
LOCUS       DAOOYB010000033        16741 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_3352_length_16741_cov_10.301870, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000033 DAOOYB010000000
VERSION     DAOOYB010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 16741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16741
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3352_length_16741_cov_10.301870"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..858
                     /gene="pyrH"
                     /locus_tag="PL056_03275"
     CDS             148..858
                     /gene="pyrH"
                     /locus_tag="PL056_03275"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004917986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HPM46072.1"
                     /translation="MLYKRILLKLSGEALLAPDGKPIDQKFLNYLSNEIKPVYENGVQ
                     IAIVVGGGNIFRGLNANAETGLDRVTGDYMGMLGTVINSLALKAAFEAAGMPSRTMTA
                     IEIDKVAEPFIRGRALRHLEKGRIVIFGAGTGNPYFTTDTAAALRAAEIGAEVLLKAT
                     KVDGLYDKDPNKYKDAVFIKRTTYQEAIEKKLRVMDMTALSLCEENGLPVKIVNIFEK
                     GNIFKALTDDKTGSMVTN"
     gene            879..1442
                     /gene="frr"
                     /locus_tag="PL056_03280"
     CDS             879..1442
                     /gene="frr"
                     /locus_tag="PL056_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017753788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HPM46073.1"
                     /translation="MFEQKAFFDDLKDKFDKSIDAYKTEIAQYRTGKATPKLLDGIFV
                     DYYGARTPLNQMASVTTPDARLILVQPYDRSVLKGVETAIRNANLGFNPVNDGANIKV
                     PVPSLTEERRKDLVKQLHQLTEKYRVSMRNLRRDALAEIKRAEKDKEITEDEEKKFSD
                     KVQKDLNDFIKKLDDIAKVKENDIIEL"
     gene            1455..2984
                     /locus_tag="PL056_03285"
     CDS             1455..2984
                     /locus_tag="PL056_03285"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HPM46074.1"
                     /translation="MSEYVLGIDLGTSTTTVSIVKDGMATVIPIEGEEEGKIMPSVVS
                     FLNEGKNILVGKKAKDRRHLNPINTIYSIKRIIGRKYSHPETIKYAKKFPYKVVGGKN
                     DSVEIEIFGKRVTPQMISSLVLRKAKKAATDYLGFEVSDAVITVPANYNEAQRRATQD
                     AGKLAGLNVLRIINEPTAAALAYGFGNNLNEKIAVFDFGGGTFDITVLEVKDNFFEVL
                     STAGDSFLGGDDIDAALVQFMVVDIEANYNISMDMNNEMMTILLAEAERIKISLSTQD
                     KIKVMIKNMIPLGEKKYADYERIIDRKLFQEIISPIIQRTFDITANAIENAHVEKNQI
                     DAVILVGGSTKIPYVQEKVTEFFGQPPYFGIETVLVISMGASILGHTLISGYSEAAPV
                     LLDVIPLSVGVGTVGDYIEIIIEKNEPLPIERTSVFTNATDDQTAVRIEIFQGDARNK
                     SESHLMGDLVLSDLRKAKRGELKIEVKFEVDTNGVLNISAVDLETGKIQKISLNILGL
                     E"
     gene            2988..3902
                     /locus_tag="PL056_03290"
     CDS             2988..3902
                     /locus_tag="PL056_03290"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HPM46075.1"
                     /translation="MKAKIIICMKDEKYLRSLEKFIVSKVKDCECTGVLEIKELSGLD
                     LSSYTLMIVSSLLSEGVWLKALPVIRKVQNFILLGVNEGPEVTDAIALKYGAAAFFKL
                     PLNSDSLLNAVNDIIERAERKAKVPDIITKDFIDSITDFFTNMDSMNYYQLFGIERTC
                     SVADMKKKYISVARKFHPDKFRNVPAEIKTMVYEITKRANEAYSVLSHPNRKVIYDKM
                     LTENPDIKRFDFRNKVAYKENPEDAVQNDQARRFAVLAKKAMDGGDFRSAITQLKMAM
                     SMEKNNSYLIKLMEEATQKVAPKPEKSE"
     gene            3921..4682
                     /locus_tag="PL056_03295"
     CDS             3921..4682
                     /locus_tag="PL056_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HPM46076.1"
                     /translation="MTENFLKDNGLTKERLPQHVAIIMDGNGRWAKKRLMNRLKGHRE
                     GADSVDRITSFCRKAGIEYLTLYAFSTENWNRPVEEVGGLMDMLDEFLNKKRETLLKN
                     NIRFNVIGDLSRFPKNITKTIKSLMNETALNDPVMTMTLCLSYGSRFEIISAAKKIAQ
                     EVLSGNVRLEDIDEKMFGDSLFTWDIPDPDLVIRTSGEKRLSNYLLWQSAYSEFYFTD
                     VFWPDFKEPEFIKALVDYASRERRFGKTAEQLKKR"
     gene            4685..5551
                     /locus_tag="PL056_03300"
     CDS             4685..5551
                     /locus_tag="PL056_03300"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HPM46077.1"
                     /translation="MLKDWKRYATGLSLMPIVLGIVYLLPDNFFLYLILIVAFFTFSE
                     YLTMFNERKQPFFILIAVIMMFMIESISGMSALMKLSSITFGEVDGLFRPLKQFFLAI
                     FCSAVLIPVFSLYGKDAIEIKFKRMLIYFFGFFYFGMTFGLFPLIRNYGTHHHWLTFA
                     LVTPWICDTAAYFGGRFFGKHKFAPAISPKKTWEGAISGTLFSMITGLIFNFTLLRNE
                     NLFFILAVSFFIGTLGQLGDLVESLIKRGASVKDSGTLFPGHGGLFDRTDSLVVSVAV
                     VFIAFLVKNYVS"
     gene            5541..6695
                     /locus_tag="PL056_03305"
     CDS             5541..6695
                     /locus_tag="PL056_03305"
                     /inference="COORDINATES: protein motif:HMM:NF014700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46078.1"
                     /translation="MCPEKIVIFGITGSIGTNSEKIISAFPDRFELIGASAGNNIELL
                     NTIIKRHPSVEAVAIASGNDHSKISDFHGTVYSGEEELIRLLDLKPDKIVMALPGKQG
                     WKITVEAVKRNIPVCLANKESLVIAGFYLGNKVTSDRSMIIPIDSEHAALMQLLENCD
                     RSRINKVYITASGGALRNMSKDEMLEADAQKALAHPVWNMGKKVTVDSATMLNKGLEL
                     FEAFWLFNIDPGKLGVLVHPSVDVHAAIMFKDGSAVSQNAVGNMLVPIAAAMSYPEML
                     PVVGKFPDMEFKFENREMKFSPPDLTKYPLLAKAFELLNKKDFSGMVAYAISDEIAVE
                     KFLKNEITVKGIHEIVAKSVEKCAGRLAPSSINKIEEFIDEIEIFAREVL"
     gene            complement(6692..>7093)
                     /locus_tag="PL056_03310"
     CDS             complement(6692..>7093)
                     /locus_tag="PL056_03310"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPM46079.1"
                     /translation="IILLTVPLSLIGSALGLMIFNQPFSFTAFLGISSLIGIVVNNAI
                     LIIEFLNMYRSKEKDIVTASKTAVSRRFRPIMLSTVTTVMGLVPLVISGSTLFEPMSV
                     SLMSGLLVSTLLTLIVVPVVYVMIEGESTLS"
     assembly_gap    7094..7193
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7194..9400)
                     /locus_tag="PL056_03315"
     CDS             complement(<7194..9400)
                     /locus_tag="PL056_03315"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPM46080.1"
                     /translation="MRSLILASVTNRRITIFLVIALVIAGFASYYIMPRQENPDLKPS
                     IALVTAVYPGAIPADVEKLVTKKIEDAASEIINFESVESFSKNSASIVTVRIDANADR
                     DKTWTDLRQKIDDIVPELPEGCWKPQIFTNLGETAGMILAISGSNYNYDQLEAFAERF
                     KERLVEVDGVRRFDIAGKIDREVVVEIESERLNSYQLSFEDVFRILAAQNIEIPSGAI
                     KFPQGRVNVNVPGIFESINDIENAIVDVSPMNGSVARIKDFAKVEMRLKDDAVKTRHN
                     GKDAILLSGYFQEDRNVVIIGKTVREILEEVKSTLPPDLEVSEVLYQPDDVKEAVSGF
                     MMNLLQGMLFVIIVVFLGMGLRNAAIVATAIPLSVLISFIVMALTGIQIHQISTTALI
                     ISLGMLVDNAIVISDAIQYRIDNGIDNINASVDGALESAVPVFTATLTTVAAFTPLLF
                     LPGQIGQYVIAVPQLVMISLTASYFVAMFVTPALASILFVPDKSAGSKKDSMIKRVFA
                     YLLNKGLLHRWKTAGLSFLIFFAVMILGFMFLQLRFFPNTDKNIVYIDIVSEMTDIDK
                     TDAITTEIEKIVSSQPEVLQYTTSVGDDIPKFYIAMMPKAPSVDYSQIMMRVDLKKGG
                     RFRSKEDFAFDLQKVLNSKVSGGKIKVKVPQQAEPMAAPVIFRVNGENPEHISKVVND
                     LEKEISEIEGTVDVRNNSLKDAYEFSVDIDPDSVSSLGILKYDVQRQVNIALKG"
     gene            complement(9397..9954)
                     /locus_tag="PL056_03320"
     CDS             complement(9397..9954)
                     /locus_tag="PL056_03320"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HPM46081.1"
                     /translation="MLYLPRRTPIKKHRIIFLNKKGEIVAAGYPVVVVRNESFVINTG
                     VPSKELVMIRQGTEADIKVDGRILKGKVSVISQVPDERSRTYNVEVEIAGKTDEAELF
                     IGSVAKVDFITGNIEGIWIPVFAILTDGTDYVYTDVDGIALRKNIRTGNISGEFVLVD
                     GLNSGDRVIVKGMKDISDGDLVKEQ"
     gene            complement(9899..10144)
                     /locus_tag="PL056_03325"
     CDS             complement(9899..10144)
                     /locus_tag="PL056_03325"
                     /inference="COORDINATES: protein motif:HMM:NF024923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HPM46082.1"
                     /translation="MSVLTVNNESRISYIRSIGMVSSKDIKKYGFKIPGKIASITVKK
                     GDRVKKGTLLARLETKDLEFALSAAENTYKKTQDYFS"
     gene            10380..11285
                     /gene="thiL"
                     /locus_tag="PL056_03330"
     CDS             10380..11285
                     /gene="thiL"
                     /locus_tag="PL056_03330"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HPM46083.1"
                     /translation="MLKNRERDIIDLISDKFNKFIGDDCAVWDERSLVVTTDHMCEGV
                     HFDLSFMFPEAVGWRLMAANASDIISMGSLPTHFLFNIAIPPDRLKTAERIIDGVKKF
                     AGKYNIEIMGGDTTRAKNFMIGATMFGEKPAKPLLRSGAKPGDRVYLADSTGLSHCGL
                     LHLKNRSKGFERSVEKFLFPSPFDSIPQNLNDINCAIDISDSLISELKLIAEMSNVSI
                     EIDYDRIPVEDEVLQTSEMFSIPVEKLVLGSGEEFILLFTSDKIIKNAFEIGTVTTKK
                     EKDVKIFSKKSMLDLCDTALFSHFG"
     gene            11215..11670
                     /locus_tag="PL056_03335"
     CDS             11215..11670
                     /locus_tag="PL056_03335"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HPM46084.1"
                     /translation="MLRSFQKKACLIFATLLFSVISAEDICSYPAEAEITVTSKTGAK
                     ISFAVGIAETPAEHEKGLMHCAELKKGKGLFFIFNDDREHFFWMKNTTVELAIIYIDR
                     DFNVVSIKRGKPLSETTLPSIFPSRYVLEVNWAEGGKILPGDKVFFKMK"
     gene            11774..13348
                     /locus_tag="PL056_03340"
     CDS             11774..13348
                     /locus_tag="PL056_03340"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HPM46085.1"
                     /translation="MQKILMITVLYTMLLFPFAVSGFSWDDVQPCQPVFIRLSFVDSD
                     ATSKMGITWQTKNECSAELRYGSDFAPDKYVKGATIKGNNDLGYIHQVTLEGLTHSTF
                     YQYQVGGENNWSILYNFTTAPFQGGCDPFAFVVASDSRVDQTAEEGNPVKWRDIFTEA
                     LNDGASFLINGGDMVWDGEKTSQWLNYLDTTSDSFAAKPLMIAMGNHDNGPGEGENAN
                     FNQLMHYPENPVTETEDSYFFRYGNALFIIISTVTYTDLFEDTAKWMDEVLTNNSDAK
                     WKFVFNHHPFYSSAGLEYLGMRIGHEMNEQGQNPFFIPIFDKHHVDFVISAHSHYYER
                     FAPSYGYIDRTDPDEIEPQIVDSFDKGTVYVVSGGGGAITFQNFLVDMLCTNSIKGSQ
                     SCNGKNHYLKFMIDNNRLVMEMWTTMRQFDGFNPNDREMVDVVEIIKADASCETPEET
                     PDEEPADNDTNDEDIEPLNDESQQDEDITESNDEDSVKVDDNTQIDEITEEPDDEDNP
                     SAETPGESGCGCTLIF"
     gene            complement(13349..13720)
                     /locus_tag="PL056_03345"
     CDS             complement(13349..13720)
                     /locus_tag="PL056_03345"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HPM46086.1"
                     /translation="MGFFDFLKLKPTVMPVELTLLNFHPSIKNSPLPVLVDFYSNTCA
                     PCAMMAKTVTKFATDFQGRIRVGAFNIEQDKEGKIVVPFKVRAVPTLIMFNKGEPVET
                     FVGVTGYLKLQEALEKIEGKK"
     gene            complement(13733..15205)
                     /locus_tag="PL056_03350"
     CDS             complement(13733..15205)
                     /locus_tag="PL056_03350"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPM46087.1"
                     /translation="MKRVIFAILFFSLNIFANDTVFVKFENNIKKFSAIEFSDCGIND
                     YYKIPRTSYYAVKTENSAKAVRCISQNRSVKKVFADEPMNISYKKAVPDPYLQNQWHF
                     QNTGQTGIAGNDAKIIEAWNYLEELGIKPGLGVKIGLIDDAFDLHHPDMEGKFLKGRD
                     VTNGSDYPYAHDNEPHGTCVAGVIAAVKDNGIGVAGACPFCRIVPVRASEELGKAENM
                     ANAFNYLLDRGVHVISNSWGPSDNSGPVEMPEIIAEIINFARFEFRKGKGIVVFFAAG
                     NGNESISDPETFDGFAASPDVIAVGAVNASGSRSAYSDFGEDLDIVSPSSDVDAGYIW
                     DPFAIDLTSDGIWTVDARWYYGYNQTDYTSTFGGTSSATPLVAAVAGLMISAYPDVTW
                     DEIYEIITESADKIAPADAGYDENGFSIYYGYGRINAVNALKTLCEKYECAGGLEESK
                     EEVYESYDQEIPDEGEFSEFPDDSMLPIEKYAYGCNITLF"
     gene            complement(15314..>16741)
                     /locus_tag="PL056_03355"
     CDS             complement(15314..>16741)
                     /locus_tag="PL056_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46088.1"
                     /translation="EANKTVCTDTNLDGVEECACVATYHLEETTCVSNTKMVDCVDNA
                     PANATSTIVPVEVTWNGTAWSTPVDCEWDCDGGYTLESGLCINSKMVNCTDAAPANAT
                     STIVPVEVTWNGTAWSTPVDCEWDCDDNFVINGTADGCEPDPCDPNPCSGGTPVCLAD
                     ETPEGYSCYPDYNVDWCIVQFPKAVEGALLGDKVDLYSRVYIPGITTVTADAIDLHPL
                     LKVQFGGAPVGTPVSEWEADTWTDATPNEGYGNDDEYMVLQHTLTIAGEFDYTFRYSV
                     DAGQTWTVCDTEGNMPYDPAKLGFASVNRIFAKWPLDTDFIADGSATLVDDAVEVTNF
                     VGGSGIGAITWGTNGAFASGWSTGALDLNDYFQVSMKVKDAFAQNIVIDNLLYGERRS
                     GTGIRDYTIRYSKTEDFSSYTDLDSFNVPDNDLTRTNRFDNIGDIVVAPGETIYFRFY
                     GYTSEAAAGTWRMINLSFGGYYQDI"
BASE COUNT         5105 a         3538 c         3370 g         4628 t
ORIGIN      
        1 caacctgttg agggaataaa ccatgaaagc gttgtggaat tcttgaaaaa cccatctctt
       61 taaaacggat atccccccgc ttccaaaaaa cttgaaaact gaaatgttat actgtaacct
      121 tgcgcgtatt taactgatgg aggtaatatg ctttataaaa gaatactatt aaaattaagc
      181 ggcgaggcac tccttgcacc tgatggaaaa ccgatagatc agaaatttct caattacctt
      241 tcaaatgaga taaagccggt ttatgaaaac ggtgtccaga tcgcgatcgt tgtcggggga
      301 ggaaacattt tcagaggtct caatgcaaat gccgaaaccg gtcttgacag agtcaccgga
      361 gattatatgg ggatgctcgg aacagtcata aacagtcttg cacttaaagc ggcatttgaa
      421 gcggcaggaa tgccttcaag aaccatgacg gcgatcgaga tcgacaaagt tgctgaacct
      481 ttcatcagag gccgtgcttt aagacacctc gaaaaaggcc gcatcgtgat attcggtgcc
      541 gggacaggca acccgtactt tacaaccgat acagcagcgg cactcagagc tgctgaaata
      601 ggagccgaag tgcttttaaa ggcgacaaag gtcgacggtc tttacgacaa ggacccgaac
      661 aagtacaaag atgccgtctt tatcaagaga acgacctatc aggaagcaat agaaaaaaaa
      721 ctcagggtta tggatatgac cgctctttcg ctttgcgaag agaacgggct tcccgttaaa
      781 attgtcaata tattcgaaaa aggcaatatt ttcaaagcac ttacagacga caaaaccggc
      841 agtatggtga caaactgaaa taatatttgg aggttaacat gttcgagcag aaagcttttt
      901 ttgatgacct taaagacaag ttcgacaaat cgatcgatgc ctacaaaacc gagatagctc
      961 agtacagaac aggaaaagct actcccaaac tccttgacgg catttttgtt gactattacg
     1021 gggcaagaac acctcttaat cagatggcgt cagtaactac tcccgatgca cggctcatac
     1081 tggtccaacc ttacgacaga tctgttctca aaggtgttga gactgcgatc agaaacgcaa
     1141 atctgggatt caatccggta aatgacggtg ctaacataaa agttcctgtt ccttcactca
     1201 cagaggaaag aagaaaggac cttgtaaaac agctccatca gcttactgaa aaatacagag
     1261 taagtatgag aaatctcaga agggatgccc ttgctgaaat aaaaagagct gagaaagata
     1321 aagagataac cgaagacgaa gagaagaaat tctctgataa agttcagaag gatctcaatg
     1381 atttcatcaa gaaactcgac gatatcgcta aggtgaaaga gaacgacatt attgaacttt
     1441 aaccggagag aagaatgtca gaatatgttc ttggaataga cctcggaact tcaacgacaa
     1501 cagtttccat agttaaggat ggtatggcca ctgttattcc gatagaagga gaagaggaag
     1561 ggaagataat gccttcagtt gtctcttttc ttaatgaagg gaagaatatt ctcgtcggga
     1621 aaaaagccaa ggacagaaga catttaaacc cgataaacac catctacagt ataaaaagaa
     1681 taatcggtcg caagtacagt catcctgaaa cgatcaaata tgcgaagaag ttcccctaca
     1741 aggttgtcgg gggaaaaaac gattctgttg aaatagagat cttcggcaaa agggtcactc
     1801 ctcagatgat ctcttcgctg gttttgagaa aggcaaaaaa agcggcaacc gattatctcg
     1861 gatttgaagt gagtgatgcc gtcataacag ttcctgcaaa ttataacgag gcacagagaa
     1921 gagcgacaca ggatgcaggc aaactggccg ggctcaatgt cctgagaata atcaatgaac
     1981 ctactgcggc ggcacttgca tacggattcg gcaacaatct taacgagaag atcgccgttt
     2041 tcgactttgg cggtggaact tttgatatca cagtacttga agttaaagac aacttttttg
     2101 aagttctttc aactgccggc gacagtttcc tcggtggtga cgacatagac gcggcacttg
     2161 tacagttcat ggtcgttgac attgaggcaa actataacat cagcatggat atgaacaatg
     2221 aaatgatgac aattcttctt gctgaagcgg aaaggataaa gatttcgctt tccactcagg
     2281 ataagatcaa ggtcatgatc aagaacatga taccgcttgg tgaaaaaaaa tatgccgatt
     2341 atgaaaggat aattgaccgg aagctttttc aagagatcat ttcacccata atccagagaa
     2401 catttgatat aactgcgaat gcgatcgaga acgctcatgt tgaaaagaat cagatcgatg
     2461 ccgtaattct tgtcggcggc tcaacaaaaa taccgtatgt acaggaaaaa gttactgaat
     2521 tttttggaca gcccccttat ttcggaatag agaccgtcct tgtaatctca atgggtgcca
     2581 gcattctcgg tcacacactg ataagcggtt acagtgaagc ggctccggta cttctggatg
     2641 taattcccct ttccgtgggt gtcggaaccg taggcgacta cattgaaata ataattgaaa
     2701 agaacgagcc ccttccgatt gagcggacaa gtgtttttac aaacgcaacc gacgatcaga
     2761 cagcagtaag aatagaaata ttccagggag atgccaggaa caagagtgaa tcccatttga
     2821 tgggggatct cgttctttct gacttgagaa aggcaaaaag aggcgaactg aaaatcgaag
     2881 ttaagttcga ggttgacact aacggagttc ttaacatcag cgctgtcgat cttgagacag
     2941 gaaaaattca gaagatttca ttaaacatac ttggtttgga gtaactgatg aaagcaaaga
     3001 tcattatttg catgaaagac gagaagtatt taagatcact agagaaattc attgtatcca
     3061 aggtgaagga ctgtgaatgc acaggagttc ttgaaataaa agaactttcc gggctggatc
     3121 tatcttccta tactcttatg atagtttcga gtcttctttc ggaaggggtc tggctgaaag
     3181 ccctgcctgt aataagaaaa gttcagaact tcattcttct tggtgtcaac gaaggtcctg
     3241 aagtgactga tgctatagct ttgaaatacg gtgcggccgc ttttttcaaa cttccactta
     3301 acagcgattc tcttctaaat gcagtaaacg acatcattga aagagccgaa cgaaaagcca
     3361 aagttcctga tatcattaca aaagatttta tcgactcgat aactgatttt tttacaaata
     3421 tggacagcat gaattactac cagttgttcg gcatagaaag aacctgctct gttgccgata
     3481 tgaagaaaaa atatatatcg gttgccagaa aatttcatcc tgacaagttc agaaatgttc
     3541 cagcagaaat aaaaaccatg gtctatgaaa taacaaaaag ggcaaacgag gcatattctg
     3601 ttctctcaca tccgaataga aaagtgatct atgacaagat gcttactgaa aatccggata
     3661 tcaaaagatt cgatttcaga aacaaggtcg cctacaagga aaatcccgaa gatgctgttc
     3721 agaacgatca ggcaagaaga ttcgcggtac tggctaaaaa agcgatggac ggcggggatt
     3781 tcagatctgc gataacacag cttaagatgg caatgtcaat ggaaaagaac aacagttatc
     3841 tcataaagct tatggaagag gcgacacaga aagttgcacc taaacctgaa aaatctgaat
     3901 aattaaaggt tactgttaaa atgactgaaa attttctcaa agataacggt cttactaagg
     3961 aacgacttcc tcagcatgtt gcaataatca tggacggcaa cggacgatgg gcaaagaagc
     4021 gtcttatgaa ccgtcttaag ggacaccgcg aaggggctga cagtgttgac aggataactt
     4081 cattctgccg caaagcaggg atagaatatc tcactcttta cgcgttttcc acagagaact
     4141 ggaaccgtcc ggttgaagaa gtgggcggac ttatggatat gcttgatgag tttctgaaca
     4201 aaaaaaggga gacacttctt aaaaacaaca taaggttcaa tgttatcggc gacttatcaa
     4261 gatttcctaa aaatataaca aagacaataa aatcacttat gaatgaaacc gcacttaatg
     4321 atccggtgat gacaatgact ctgtgcttaa gttacggatc ccgttttgag atcatttcag
     4381 ccgcaaaaaa gatcgcacag gaagttcttt caggaaatgt cagattagag gatattgatg
     4441 aaaaaatgtt tggcgattcc ctttttacct gggatattcc tgatcctgac ctcgtaataa
     4501 gaacaagcgg agaaaagcgg ctttcaaatt atcttttatg gcagtcggca tattcagaat
     4561 tttattttac agatgttttc tggcccgatt tcaaagaacc ggaattcatt aaagcccttg
     4621 ttgactatgc ttcgagggag agaagattcg gaaaaacagc tgaacaattg aaaaagaggt
     4681 gaaaatgcta aaagactgga aacgatatgc cacagggctt tcacttatgc ccattgtgct
     4741 ggggatcgtc tatcttcttc ctgacaattt ctttctttat ctgatactta ttgttgcctt
     4801 tttcacattt tccgaatatc tgacaatgtt caatgaaagg aaacaacctt ttttcattct
     4861 tattgctgtc ataatgatgt tcatgatcga atctataagc ggaatgagcg cgctgatgaa
     4921 actttccagc ataactttcg gtgaggtcga cggattgttc cgtcctctga aacagttctt
     4981 tcttgccatt ttctgttcgg cagttctgat accggtcttt tcactttacg gaaaagatgc
     5041 aatagaaata aaattcaaaa gaatgctgat atatttcttc ggctttttct atttcggcat
     5101 gacattcggg cttttcccgc tgatccgcaa ttacggaact caccaccact ggcttacatt
     5161 cgccctcgta accccatgga tctgcgatac agccgcatat ttcggaggga gatttttcgg
     5221 aaaacacaaa tttgctccgg ccatttcacc gaaaaagaca tgggaaggtg caatttccgg
     5281 aacactgttc tcaatgatta caggactgat ctttaatttc actcttttaa gaaatgaaaa
     5341 cctgtttttt attcttgcag tatcattttt tatcgggaca ctcggacagc tcggtgatct
     5401 tgttgaatcg ctcataaaaa gaggagcaag cgttaaagat tcgggaactc ttttcccggg
     5461 tcacggcgga ctttttgaca gaaccgattc ccttgttgtt tctgtagctg ttgtgttcat
     5521 cgcatttctt gtgaaaaatt atgtgtcctg aaaaaatagt aatattcgga ataaccggat
     5581 caatcggcac aaattcagaa aagataattt cagcttttcc tgacagattt gaacttatcg
     5641 gtgccagtgc aggaaacaat attgaattac ttaacaccat cattaaaaga cacccctccg
     5701 tcgaagctgt tgcaatagct tccggaaatg atcacagcaa aatctcagat tttcacggaa
     5761 ctgtttattc aggtgaagag gaacttatcc ggcttcttga cctcaagccg gataaaattg
     5821 taatggcgct tcctgggaaa caggggtgga aaataaccgt agaagcggta aaaagaaata
     5881 ttccagtctg ccttgcaaac aaagaatctc tagtaattgc agggttttat cttggaaaca
     5941 aggtaacatc cgacaggtca atgatcattc cgatagacag tgaacatgcc gcattgatgc
     6001 aacttttaga gaactgtgac agatcaagga taaataaagt ttatattacc gcttccggtg
     6061 gtgccttaag aaatatgtca aaagacgaaa tgcttgaagc cgatgcacaa aaagcacttg
     6121 cccaccctgt ctggaacatg ggaaaaaagg tgacagttga cagcgcaaca atgctgaaca
     6181 aagggcttga actttttgaa gctttctggc ttttcaacat tgatcccgga aagctcggag
     6241 ttcttgttca tccgagcgtt gatgtccatg ccgcaataat gttcaaagac ggctcagcgg
     6301 tgtcgcagaa cgctgtcggc aacatgctcg ttcctattgc cgccgcaatg agctatcctg
     6361 aaatgcttcc ggtagttggg aaatttcccg atatggagtt caagtttgaa aaccgtgaaa
     6421 tgaagttttc tccgcctgat ctgacaaaat atccccttct agccaaggca tttgaattgt
     6481 tgaataaaaa agatttctcg ggaatggtcg cctatgcgat atcagatgag atagctgtgg
     6541 aaaagttcct taaaaatgaa attactgtga aaggaatcca tgagatcgtc gcaaagtccg
     6601 tcgaaaaatg cgcaggcagg ttggcacctt catcaattaa taaaatagaa gaatttattg
     6661 atgaaataga aatcttcgcc agagaagtcc tttaagacag tgtgctttcc ccctctatca
     6721 ttacatacac aactggaaca acgatcagcg taagaagtgt cgaaacaagc agtccgctca
     6781 ttaaactgac agacatcggc tcaaaaagag tgctccccga tattacaagc ggcaccagcc
     6841 ccattacagt tgttacagtg ctcagcatta ttggtctgaa ccttcttgaa actgctgttt
     6901 ttgaagctgt tacgatatct ttttctttcg atctgtacat gttaaggaac tcgattatta
     6961 atatcgcatt atttacaact attcctatca gacttgatat tccaagaaac gcggtaaatg
     7021 agaacggctg gttgaatatc atgagtccaa gggctgaacc gataagcgac aatggaacag
     7081 taagaagaat aatnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncctttta
     7201 aagcgatgtt cacctgcctt tgaacatcat atttgagaat tccgagactg ctgacggagt
     7261 cgggatcaat atcgacggaa aattcatagg catctttcag ggagttgttt ctcacatcaa
     7321 ctgtcccctc aatttcagag atctcttttt ccagatcatt gaccactttt gaaatgtgtt
     7381 cggggttttc accgtttact ctgaatatga caggagccgc catcggttcg gcctgctgcg
     7441 ggactttaac cttgatcttt ccaccgctga cttttgaatt gagaactttc tggagatcaa
     7501 aggcaaaatc ctcttttgat ctgaaccttc cccctttttt aagatccacc ctcatcatta
     7561 tctgtgagta atcaactgaa ggggctttag gcatcatggc aatgtagaat ttcgggatat
     7621 catctcctac ggatgtcgta tattgcagaa cttccggctg agagctcaca attttctcga
     7681 tctcagttgt aattgcatct gttttgtcaa tatctgtcat ttcagaaaca atatctatat
     7741 aaacaatgtt tttgtctgta ttgggaaaga atctcagctg taaaaacata aacccgagta
     7801 tcatgacagc aaagaatatt aaaaaggaaa gtcctgccgt tttccatctg tggagaagtc
     7861 ctttattcag aagatatgca aaaactcttt ttatcatcga atcttttttt gaacctgcac
     7921 ttttatcagg aacaaaaagt attgaagcta aagcaggagt cacgaacatt gccacaaaat
     7981 atgaagcggt gagggaaatc atgacaagct ggggaactgc aatgacatac tgcccgatct
     8041 gtccgggaag aaaaagaaga ggggtaaaag ccgcgacagt tgtcagggtt gcggtaaaaa
     8101 caggaaccgc tgattccaaa gcaccgtcaa cagaggcatt gatgttgtca ataccgttat
     8161 ctatcctgta ttgtatagca tctgatatga caatggcatt atcgacaagc attccaagtg
     8221 aaattataag cgcggttgta gatatctggt gtatctgaat gccggtcagt gccattacaa
     8281 tgaacgaaat aagtactgac agaggaatag ctgttgcaac gatagccgca tttctcagtc
     8341 ccataccgag aaaaaccaca attataacaa aaagcatccc ctgaagaaga ttcatcatga
     8401 aaccgcttac cgcttctttc acatcatccg gctgatagag aacttcagac acttcaagat
     8461 caggaggaag tgtcgatttt acttcttcaa ggatctctct taccgttttt ccgattatca
     8521 caacatttct gtcttcctga aaatatcccg aaagcagaat ggcatcttta ccgttatgcc
     8581 ttgtctttac cgcgtcatcc ttcagacgca tctcaacttt tgcgaaatcc tttattctcg
     8641 cgacagatcc attcatgggt gaaacatcaa ctattgcgtt ttcaatatcg tttattgatt
     8701 caaaaatgcc cggaacattc acatttaccc ttccttgcgg gaattttata gcaccggaag
     8761 gtatctcaat attctgggcg gcaagtatcc tgaaaacatc ttcaaacgaa agctgatagc
     8821 tgttaagccg ctcagattcg atttcaacca ctacttcacg atcgatcttg cctgcgatat
     8881 cgaaccttct gactccgtca acttcaacga gccgctcttt gaacctttcg gcaaaagctt
     8941 cgagctggtc gtaattataa ttcgacccgg aaatggcaag tatcattcct gccgtttcac
     9001 cgagatttgt gaatatctgc ggtttccagc acccttcagg aagttccgga acaatatcat
     9061 cgatcttctg cctcagatct gtccaggtct tgtctctatc agcatttgca tcaattctta
     9121 cagtgacaat tgaagcactg ttctttgaaa aactttcaac actttcaaaa tttattatct
     9181 cactcgcggc atcctctatc ttttttgtca caagtttctc aacatcagca gggatcgccc
     9241 ccggatatac tgcggtaaca agagcgattg aaggtttaag atcagggttt tcctgtctcg
     9301 gcatgatgta ataacttgca aaaccggcaa ttactaatgc gataacaaga aaaatggtta
     9361 ttctgcgatt agttaccgat gcgagaatta aagatctcat tgctctttta ccaggtcacc
     9421 gtcactgata tctttcatcc cttttactat gaccctgtca ccggagttca aaccatcaac
     9481 aagaacaaat tcacctgaaa tattacccgt tcttatgttc tttctcaaag cgatgccgtc
     9541 aacatcagta taaacatagt ctgtaccatc cgtaagaatg gcaaaaaccg ggatccagat
     9601 cccttcaata tttcctgtaa taaaatcaac tttggcaaca gaaccgatga aaagctctgc
     9661 ttcatcagtt ttccctgcta tttcaacctc gacattgtat gtcctgctcc tttcatcagg
     9721 aacctgagaa ataaccgaaa cttttccctt aaggattctg ccgtcaactt ttatgtcggc
     9781 ctctgtcccc tgtcttatca ttacaagctc ttttgaagga acgccggtgt ttatcacaaa
     9841 cgattcattc ctaaccacca caaccggata acctgctgca acaatttccc cttttttatt
     9901 aagaaaaata atcctgtgtt tttttatagg tgttctccgc ggcagataaa gcaaattcaa
     9961 gatcttttgt ctcaagtctg gcaaggagtg tccccttttt cactctgtca ccttttttca
    10021 cagtaattga tgcgatcttt ccggggatct taaaaccgta tttcttaata tcttttgaag
    10081 aaaccatccc gatcgatctg atatatgaaa tccttgattc gttattgacc gtaagaaccg
    10141 acaccggctt tattctctgc tctttttttt cttcctgtaa accgcatgaa agagtcaaca
    10201 aagaggttca gattacaata aaaacgaatc ttttcattcc tttactccca aaacaccttg
    10261 aaatttctca gtgtttgcga tatttaattt atttaatgca gatatgtcaa atttttcacc
    10321 ttcaaatatt gcacttcaat agttgatgaa tattatagaa aaggttttca ggaggaagta
    10381 tgttaaaaaa cagagaacgg gacataatcg atctgatctc agacaaattc aataagttca
    10441 tcggtgatga ctgtgcagtt tgggatgaaa ggtctttagt cgtgaccaca gaccacatgt
    10501 gcgaaggtgt tcatttcgat ctttcattca tgttccctga agctgtcgga tggcgtctta
    10561 tggcggcaaa tgcaagcgac ataatttcca tgggatcact gccgacccat tttcttttca
    10621 atatcgcaat tcctcctgac cggctgaaaa cagcggaaag gataattgac ggagttaaga
    10681 aatttgcagg taaatataac atagaaataa tgggcggaga cacaacgaga gcaaagaact
    10741 tcatgatcgg agccacgatg tttggtgaaa agccggcgaa accacttctc agaagcggtg
    10801 caaaaccggg tgacagagtg tatcttgctg attccacagg actttcccac tgcgggctcc
    10861 ttcatcttaa aaacaggtca aaaggttttg aaagatctgt ggagaaattt ctgttcccct
    10921 ccccttttga ttcaattccg caaaatctta atgacataaa ctgtgcaatc gatatcagtg
    10981 attcgctcat ttcggaactg aaactcattg cagaaatgtc taatgtttcc atagagatag
    11041 attatgacag aatacctgtt gaagacgaag tgctgcaaac ttcggaaatg ttctccatcc
    11101 ctgttgaaaa gcttgtcctc ggcagcggag aggagttcat tcttcttttc acatcggata
    11161 aaattataaa aaacgccttt gagataggaa ctgtcactac gaaaaaggag aaagatgtta
    11221 agatcttttc aaaaaaaagc atgcttgatc tttgcgacac tgctcttttc agtcatttcg
    11281 gctgaagata tatgcagtta cccggctgaa gctgaaataa ccgtcacttc gaaaacaggt
    11341 gcaaaaatat cttttgcagt cgggatcgct gaaacaccgg cagaacacga aaaagggctt
    11401 atgcactgcg ctgaacttaa gaaagggaaa ggacttttct ttattttcaa tgacgacagg
    11461 gaacattttt tctggatgaa aaacacaact gttgaactcg caattattta tatagacagg
    11521 gattttaatg ttgtttccat aaaaagggga aagccgctga gcgaaacgac tcttcccagc
    11581 atctttcctt caagatatgt ccttgaagtc aactgggcgg aagggggcaa aatacttccc
    11641 ggagataaag ttttctttaa aatgaaatga cattctgtta acaatttaaa tatttttctg
    11701 ttaggaacct taacatttct atttttcaca ctataatgcg acatgtgtgt ctttttttaa
    11761 ggaggttttc ccaatgcaga aaatattaat gatcaccgta ctttatacga tgttattatt
    11821 cccgtttgct gtttcaggtt tcagctggga tgatgttcag ccgtgtcagc cggtgttcat
    11881 acggctttct tttgtggatt cagatgccac atccaaaatg ggaataacat ggcagacaaa
    11941 gaatgaatgc agtgctgaac tcagatacgg aagtgatttt gcccctgaca aatatgttaa
    12001 aggagccacg ataaagggaa acaacgatct tggatatatt catcaggtga ctctggaagg
    12061 acttacccac tcaacttttt atcagtatca ggtcggcgga gaaaataact ggagcatact
    12121 ttataatttc actacggcac cttttcaagg gggctgtgat ccgttcgctt tcgttgtagc
    12181 gagtgacagc cgcgttgacc agactgcgga agaagggaat cctgtgaaat ggcgggacat
    12241 ctttacagaa gcattgaatg atggtgcctc ttttctgata aacggcggtg acatggtgtg
    12301 ggacggcgaa aaaacatctc agtggctcaa ctatcttgat actaccagtg acagctttgc
    12361 ggcaaaaccg cttatgatcg ccatgggcaa ccatgacaac ggtccgggag aaggagaaaa
    12421 tgctaatttc aatcagctta tgcactatcc agaaaaccct gtgaccgaaa cagaagacag
    12481 ctatttcttc aggtacggaa atgctctctt cattatcatt tcaacagtaa catatacaga
    12541 tctttttgaa gataccgcaa agtggatgga tgaagtgctt acaaacaaca gtgatgcaaa
    12601 atggaaattt gtattcaacc atcatccgtt ctattcgtcg gcaggtcttg aatatctcgg
    12661 aatgagaata ggacacgaga tgaatgaaca gggtcagaat ccgtttttca ttccaatctt
    12721 tgataagcat catgttgact ttgtgatatc ggcacacagt cattattacg aaagattcgc
    12781 tccaagctac ggatatatcg acagaactga tccagacgag atagagcccc agatagtcga
    12841 cagctttgac aagggaacgg tctatgttgt aagcgggggc ggcggagcaa taacttttca
    12901 gaattttctt gttgacatgc tttgtacaaa ttcaattaaa ggaagtcagt cctgcaacgg
    12961 aaagaaccac tatcttaaat tcatgatcga taacaaccgt cttgtgatgg aaatgtggac
    13021 aaccatgaga cagtttgacg gattcaaccc taacgaccgt gaaatggttg atgtagttga
    13081 gataattaaa gctgatgcca gctgtgaaac acctgaagaa actcccgatg aagaacctgc
    13141 ggacaatgat accaatgatg aagatatcga acccctcaat gatgaaagtc aacaggatga
    13201 agatattact gagtcaaatg atgaggattc tgttaaagtt gacgataata ctcaaattga
    13261 tgaaatcacc gaagaaccgg acgatgaaga taatccatca gctgaaacac cgggtgaatc
    13321 gggatgcggc tgcactttga tcttttaatt atttctttcc ttcgatcttt tcaagagctt
    13381 cctgaagttt cagataacct gtgacaccga caaatgtttc aaccggctct cctttgttga
    13441 acatgataag tgtgggaacc gctctaacct tgaacggaac aactattttc ccctctttat
    13501 cctgttcgat attgaaagct cccaccctga tcctgccttg aaagtctgtt gcaaattttg
    13561 tgacagtttt agccatcatt gcacatggag cacatgtatt tgaatagaaa tcgaccagaa
    13621 cgggaagagg tgaattcttg attgatgggt gaaaattaag cagtgtcagt tcaacaggca
    13681 tcaccgtcgg tttcagtttt aaaaaatcaa aaaatcccat aattttctcc ttttaaaata
    13741 aagttatatt acaaccgtaa gcatatttct ctatcggaag cattgagtcg tcaggaaact
    13801 cagaaaactc accttcatcg ggaatctctt gatcgtatga ctcataaact tcctctttgc
    13861 tctcctcaag accaccggca cattcatatt tctcacacag cgttttaagg gcattaactg
    13921 catttatcct tccgtaaccg taataaatgc taaacccgtt ctcatcatat ccggcatctg
    13981 ccggggctat cttatcagcc gactctgtaa ttatttcata tatttcatcc catgtaacat
    14041 caggatatgc agatatcata agtcctgcaa ctgctgcaac aagaggagtt gcactgcttg
    14101 ttccgccgaa agttgaagtg tagtcagtct gattgtaacc gtaataccac cttgcatcaa
    14161 ctgtccagat accgtcactc gtaaggtcaa ttgcaaaagg atcccatata tagccggcat
    14221 caacatctga agaaggggaa actatgtcaa gatcttcacc gaaatcactg taggcagatc
    14281 ttgatccgct tgcattcact gctccgactg caattacatc aggacttgcg gcgaatccgt
    14341 caaaagtttc aggatcagat atgctttcat tgccgttacc tgccgcaaaa aagacaacga
    14401 ttcctttacc ttttctgaat tcaaatcttg caaaattgat tatttcagca attatttcag
    14461 gcatttcaac tggtccactg ttgtcactcg gcccccagct gtttgaaatt acatgaacac
    14521 ctctgtcaag aagataattg aaagcgtttg ccatgttctc cgcctttcca agttcttccg
    14581 aagcccgaac cggaactatt ctgcagaatg gacatgcccc tgcaactccg atgccgttgt
    14641 ccttaactgc cgcgataact cctgcgacac atgtcccgtg cggttcattg tcgtgggcat
    14701 acggataatc cgaaccgttc gtaacatccc ttcctttaag aaattttcct tccatgtcgg
    14761 ggtgatgaag gtcaaaagca tcatcaataa gcccgatctt tactcctaaa ccgggcttta
    14821 taccaagttc ctcaagataa ttccacgctt caattatttt tgcatcattc cctgcaatgc
    14881 cggtctgtcc tgtgttttga aaatgccact gattttgtaa ataagggtcg ggaactgctt
    14941 ttttgtatga aatgttcatc ggttcatctg caaaaacctt ttttaccgac ctgttttgag
    15001 atatgcatct gaccgctttt gcactgtttt ctgtttttac agcataatat gaagtacggg
    15061 gaatcttata ataatcattg attccgcaat ctgaaaattc tatcgcagaa aacttcttga
    15121 tattattctc aaatttcaca aaaacagtat cgttagcgaa aatattcaaa ctgaaaaaaa
    15181 gtatagcaaa aataacccgt ttcattccat tccctcgaaa caactgaaag attgtacagc
    15241 aaaatcagtc aaggtaaagc aaaaaaaagg ggagccaatt gctccccctg cgtaaaaaat
    15301 gttatcaaat gacttaaata tcctgataat atccaccgaa tgaaagatta atcattcgcc
    15361 atgtcccggc agctgcttca gaagtgtatc catagaatct gaaatagatc gtctctcccg
    15421 gagcaacgac aatatcacca atgttgtcga atctatttgt tctggtcaaa tcgttatccg
    15481 gcacattgaa gctgtcaagg tctgtatatg aagaaaaatc ctccgttttt gagtaacgga
    15541 tagtgtaatc ccttattcct gtgccagaac gcctctcacc ataaagaaga ttatcaatga
    15601 cgatattctg ggcaaaagca tctttgactt tcattgaaac ctgaaaatag tcattcagat
    15661 ccaaagcccc agttgaccat ccacttgcaa aagcaccgtt tgttccccat gttattgctc
    15721 cgattcctga tccaccgaca aaattggtaa cttcaacagc atcatcaact aaagtagcac
    15781 tcccatcagc tatgaaatca gtatcaagcg gccattttgc aaaaattctg tttacagatg
    15841 caaacccaag ctttgccggg tcatacggca tgttaccttc tgtgtcacag actgtccatg
    15901 tctgtccggc atctacactg tatctgaatg tataatcaaa ttcacctgct attgtgagag
    15961 tgtgctgaag caccatatat tcatcatcat tgccgtaacc ttcattggga gttgcatcag
    16021 tccatgtgtc agcttcccat tcagaaaccg gagtgcctac cggtgctccg ccgaattgaa
    16081 ctttaagcag tggatgaaga tcaattgcat ctgcagtaac agtagtaatt cctggaatat
    16141 acactctgct gtaaagatca actttatcac ctaaaagagc tccttcgaca gctttcggaa
    16201 actgaacaat acaccagtca acattataat ccggatagca ggagtatcct tcaggtgttt
    16261 catctgcaag acaaaccgga gttccaccac tgcaaggatt cggatcacaa ggatcaggct
    16321 cacaaccgtc agcggttcca tttatcacaa aattgtcatc acaatcccat tcacaatcaa
    16381 ccggggtcga ccatgctgtc ccgttccatg ttacttcaac aggcacaatc gtgctcgtcg
    16441 catttgcagg agcggcatct gtacaattaa ccattttact gtttatgcat aatccgcttt
    16501 caagagtgta acctccgtca caatcccatt cacaatcaac tggagtcgac catgctgtcc
    16561 cgttccatgt tacttcaaca ggcacaatcg tgcttgttgc atttgcaggt gcattgtcaa
    16621 cacagtcaac catttttgta ttgcttacac atgttgtttc ttcaaggtga tatgttgcaa
    16681 cacaagcaca ttcttcaaca ccgtcaagat ttgtgtctgt gcagacagtc ttgttcgctt
    16741 c
//
LOCUS       DAOOYB010000034        16710 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_3369_length_16710_cov_9.932273, whole genome shotgun sequence.
ACCESSION   DAOOYB010000034 DAOOYB010000000
VERSION     DAOOYB010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 16710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16710
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3369_length_16710_cov_9.932273"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="PL056_03360"
     CDS             <1..428
                     /locus_tag="PL056_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HPM46089.1"
                     /translation="LATNPGLFSGLMLSQYTADTLIVEQRILCTPAAIQSIPAAADQE
                     DFVSMGMNTAIKNRQIYENACGVLGIEFMAASQALDIRKYENEGKGVAAAKKKIREHV
                     EFLEIDRPLYPDHTEMQKLVMSGEILKAVEEECVFPSDE"
     gene            complement(435..1688)
                     /locus_tag="PL056_03365"
     CDS             complement(435..1688)
                     /locus_tag="PL056_03365"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HPM46090.1"
                     /translation="MKIIDFVQLLREIYGKKEPDLEKIEKMGLLAVKIAQHFALRIDF
                     LDESVCRHLMKLYGHASKLDRTVSESLLREVTPDGWEKNFARLDYNPFTAASVGQVHN
                     ALLHDGTEVIVKLIKSDYLENFLYDIKRLKRLMKTAIFFYPKLERVFDPVAILDYIAQ
                     YTTQELNLVDEYNGQQTLMKLADDNRHRYDFSKLKFHKIYREMTGERYLVSERIKGKT
                     LDDLIVEGKVTYQMILDLFQIHGFYLFAAGTFHGDIHPGNVMVDDDGYFYFIDTGAVS
                     SVSKRMRVGLFRFFESLSQFDYETSAERIQLMATTTISGKKLDSYKVKFMELYKDFAG
                     STVSEVSLTKKMMETIKLAVHSGMRFEKDMFSIIKSMMYLDGMVLRVNPKAQLMKDMQ
                     PFIGAFNKVLSEMDAEENKIDHSQI"
     gene            complement(1693..3171)
                     /gene="crtI"
                     /locus_tag="PL056_03370"
     CDS             complement(1693..3171)
                     /gene="crtI"
                     /locus_tag="PL056_03370"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006829101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HPM46091.1"
                     /translation="MSKKVIVIGAGPGGLSSAMILASKGIDVTVYEQKSYPGGRNGFI
                     SRNGFTFDIGPTFLMMLNILEDVFKMSGKKLTDHVKITELEPMYRLSFDGGKKEFFPS
                     RDHEKMKSEIEKFSPGSFEGYKKYLKDEARKYRKIFPILRKPFNSVTDLLDWELLSSG
                     PSATPLKTLSDVIGSYYKDEYMQKAFTFQGKYIGMSPWEAPGLFSILSYVEHGGGIFH
                     VEGGLNKISQAMANIVQESGGKIHFNTKVSEIIVEDGTAKGVLLENGTIDKSDFVIIN
                     ADFAHAMTHLVEERHRKKYNDRKIASLEYSCSGFLMYLGVDGIFDDLPHHNILFADDY
                     RNNIDDIVKRKVISDDLSIYVQNASVVDKTVAPNGKSTLFVLVFMPNNTSGIDWEKEK
                     GPLREKVLDKLENQRFQGLRQKIEEEVIITPTDWEKDYSVYSGAIFNISHKLKYMLYF
                     RPHNKFEDIGNCYLVGGGTHPGSGLPTIYESGRISAELILRE"
     gene            complement(3211..4122)
                     /locus_tag="PL056_03375"
     CDS             complement(3211..4122)
                     /locus_tag="PL056_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPM46092.1"
                     /translation="MAEEKKISVVSTENGKTKPMDDSKEIRTLLMKSINSLNALDAAI
                     KEISQKVNRKDQGEANRFYITFVILGIVMILAFFFYFRAEVVKVAEKYTLMQEHNEYL
                     EKEVEELKEKLFSLENNDIKAYNLYLALKEGDPDHAFKLYGEFNLSALSRLERLIIDK
                     ETSLIKQKAAVQKYDEGEILFKRKSYEAAVDRFKESLEISSTGDHIPNLFYLTSLSYY
                     RMKDYNKAAIAFERFLFINTKKDYQKDRAELLLGVCYEKLNQWDRAKNFYIQSMKDNR
                     YNRYYPTIRDRLKNIEKKLEKQKAGED"
     gene            4245..5171
                     /locus_tag="PL056_03380"
     CDS             4245..5171
                     /locus_tag="PL056_03380"
                     /inference="COORDINATES: protein motif:HMM:NF014114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HPM46093.1"
                     /translation="MTKGFFEFKPVGFRGEVAIVAPSSPFDRTLFNAGITELKLNKLR
                     PVIDKNVFLKESFLAGNDTSRAELLINSFSKESVEAVWTARGGYGSIRLLEILERNIE
                     KIKSNPKLFIGFSDVTAIHSWLVEKCGFVTVHGPNITTLSTVDKYSKIQIFEILSGAE
                     RAFSIFSKNIRAVNHGRSRGMVKGGNLTTVASLLGTPFEPDFTNCIMFLEEVGEVPYR
                     VDRLITQLRLAGKFKGIKGLILGDFSYREFRPPAEKSVNPYAIIESAGIERNVPAICN
                     FPSGHGNQNMAFLLGAIAELDTESRTLKYTFS"
     gene            5186..5497
                     /locus_tag="PL056_03385"
     CDS             5186..5497
                     /locus_tag="PL056_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46094.1"
                     /translation="MGSNKKTREEMIELAYALQNAVTALCSKRPLRITTNKDNSILRI
                     VWMKGYEKKEWYNAAGMKILTAAFTDKHGIHVKEITVDECNHSAVYLTKKSTDPERTG
                     R"
     gene            5498..7093
                     /locus_tag="PL056_03390"
     CDS             5498..7093
                     /locus_tag="PL056_03390"
                     /inference="COORDINATES: protein motif:HMM:NF025418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HPM46095.1"
                     /translation="MKRVFVPVFILFFLLSAKADCYSGFIPKMRLSHSNSKECLYNPL
                     IHINSEDRVAIDYLETVYGEPKDSFTILPRGFYDTVKYSNDPYRIKPFLNGVETSNYW
                     QIISSAGVEYYYSNRAANFIPGSSGKIFQHPSTFVMSQSGNMVLLNSLALYWELREYN
                     YFDNSSNWESFSVDFNRVYAKLKLWKISAMFGKDALHLGPGEHGMILSANAAPYWMMK
                     FQNEETIRLWGSWNFVIMKGWLKDEREDVSNPEIFAMRMTYRPQGSFDFFELGMTRTM
                     MYSGDGMYHYKIVDYPVLISGAKDNLPRGKFDADSYGGIDFTFNIPFYKLVPELKVFK
                     LYFEESGTDIKAIWQVEDLGDFTIPYILFKFYERAYLTGIFIGLENDAMRLEYTKTAF
                     SFYKHHLYPHEGYSYRGMSLGHPLGNNHQALKFNHTHWFENGLSFKWEIGYYQLPGIN
                     RNDKDKSFSALFPMFSLDEGLIRRGYFSLWAGWVVKGHILRGYFSLDGGPKSDSDPRP
                     SKITVSDKATVDITLGFSFLLKF"
     gene            7187..7498
                     /gene="rplU"
                     /locus_tag="PL056_03395"
     CDS             7187..7498
                     /gene="rplU"
                     /locus_tag="PL056_03395"
                     /inference="COORDINATES: protein motif:HMM:NF013025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HPM46096.1"
                     /translation="MYAMIDFGGNQLKVVPGEKVLVYNLNKKEGELVENTNVLLFSDG
                     ENVSIGKPYLTDVKVKLEVVKNFKGKKVIVFKKRRRKTSKVKNGFRQSFTQLKVIEIV
                     K"
     gene            7515..7784
                     /gene="rpmA"
                     /locus_tag="PL056_03400"
     CDS             7515..7784
                     /gene="rpmA"
                     /locus_tag="PL056_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013515775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HPM46097.1"
                     /translation="MAHKKSGGSAKNGRDSAGQRRGIKKFGGEPVIAGNIIVRQVGTK
                     WIPGKNVGLGTDYTIFALCDGVVEYQTFRKNGTERKRVNVLPLEV"
     gene            7858..8901
                     /gene="obgE"
                     /locus_tag="PL056_03405"
     CDS             7858..8901
                     /gene="obgE"
                     /locus_tag="PL056_03405"
                     /inference="COORDINATES: protein motif:HMM:NF008954.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HPM46098.1"
                     /translation="MRFVDQAVITVTGGNGGAGAVSFRREKFVPLGGPDGGDGGKGGD
                     VILEGDENITTLYDVRYQKNIKAESGANGMGANMYGRGGKNETVKIPVGTLVYDNSTG
                     ELIADVTKHRQKVVVASGGIGGRGNVKFKTSVNKAPRHAQSGIAGESKIIRLELKLMA
                     DVGIIGYPSVGKSTLISVISNARPKIAEYHFTTLTPNLGMVEGPDFIPYVVADIPGLI
                     EGAHEGLGLGLQFLRHVERSRFLIHLVEINPARTSPVDDIITINRELELFNSDLAKRD
                     QIYVLNKTDIYGSEHEELRLELKKFVEGKTYFEISGVTREGVKDLMAFVGRMVAEYKL
                     KEKVDQSELKESI"
     gene            complement(8874..9239)
                     /locus_tag="PL056_03410"
     CDS             complement(8874..9239)
                     /locus_tag="PL056_03410"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HPM46099.1"
                     /translation="MMGRDNRNFRRALISLKIRLTESGDDISIKFDTHNLSEGGLFVK
                     SSILWEPEQEFDLSFFLPNCEKEIKVKGKVARSDDKYSIFVPSSEDSSMPGMGIRFLN
                     LSQEDRETIKNYIDSLSSD"
     gene            complement(9254..9598)
                     /locus_tag="PL056_03415"
     CDS             complement(9254..9598)
                     /locus_tag="PL056_03415"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HPM46100.1"
                     /translation="MFRKILKSKIHRARITGADIEYQGSITIDKNLIELADLKPFEFV
                     DIWDISNGARFETYVIEGKEGSGEICINGAAARLVAKNDLVIIASHSYIDDASQIPEP
                     KLVFVDSDNKPV"
     gene            complement(9673..10215)
                     /locus_tag="PL056_03420"
     CDS             complement(9673..10215)
                     /locus_tag="PL056_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015791337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HPM46101.1"
                     /translation="MKYEVRFSGSGGQGVILASVILGTAAALFEKKHAVQSQAYGPEA
                     RGGASKADVVISDNPVKYPKAREIDAMVCLTQKAANKYISTMKKGGVVILDSDYVDLP
                     ECENYKLIKLPMSSKTLEKQGKLIALNVVSLSALVGITKIVSFESLEKAVLESAPKGT
                     EKFNLEAVAIGRELAQNSGN"
     gene            complement(10212..11036)
                     /locus_tag="PL056_03425"
     CDS             complement(10212..11036)
                     /locus_tag="PL056_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HPM46102.1"
                     /translation="MFDYSKYLRNEKLPHIWCPGCGHGIVMKSLLRAIDKTGWSKDEI
                     VLVSGIGCASRLPGYVDCNTLHTAHGRALAFATGIKLAKPKMHVIVVSGDGDALAIGG
                     NHFIHACRRNIDITLIVFNNGIYGMTGGQYSPTTADGDKTATSPYGNVEPHFDVVDLA
                     TGAGATFVARGTSYHVPQLDTYIHKGLMHKGFSVIDVTEACYTTHGRKNKKKYGSNLD
                     MLKYQKDNAISIEKAGGMSEEELAGKIVTGVFCDKEKLEFTDKYQKIIDSVSGGAK"
     gene            complement(11054..12187)
                     /locus_tag="PL056_03430"
     CDS             complement(11054..12187)
                     /locus_tag="PL056_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HPM46103.1"
                     /translation="MTKENYEFIQGNIACAKGAAYAGCSFFGGYPITPSTEIAEFMAE
                     WLPKNGGKFIQMEDEIASIGSIIGASLTGSKSMTSTSGPGFSLMQELIGYGCIAEVPI
                     VVVNIMRGGPSTGLPTSPAQSDLLQAKWGTHGDHPTIALVPNSVEEMFTETVRAFNLA
                     EYFRTPVIVLGDEIIGHMRERIRIPDPGEIEIINRTVPNSDGLCPLPYASDGYKPACL
                     PNFGKSRFHVTGLNHDDSGFPTMDPEIVARENERIIRKVYTQRNLVDKFEAIHCEDAK
                     ILMLAVGSAARAAEESALELRKKGVKAGLFRPITLWPFPKEEFAKYALNADFIVVPEM
                     NLGQLKTVAERFTKRNKIIPVNLVNGEPLGPETIISAISHLNI"
     gene            complement(12184..12432)
                     /locus_tag="PL056_03435"
     CDS             complement(12184..12432)
                     /locus_tag="PL056_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPM46104.1"
                     /translation="MGKKEFVEIKYENAKCTLYIRPELCKGCEVCIEFCPVKALSLDK
                     KKLIVTADNMEKCIACGMCELRCPDFAIYIEKKEGAKK"
     gene            complement(12442..12945)
                     /locus_tag="PL056_03440"
     CDS             complement(12442..12945)
                     /locus_tag="PL056_03440"
                     /inference="COORDINATES: protein motif:HMM:NF017494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase C-terminal domain-containing
                     protein"
                     /protein_id="HPM46105.1"
                     /translation="MIFSNGMSEEALNAGMVTRITGELITVRDQSLKKIIEMVEKGEP
                     LPFEMKGAYIFFAAPTPGYTEHRASIGPTTSWRMGAFIPFLLELGVKGFIGKGFLPLG
                     VPEAIRRHKALYFQALGGAGAYYGSRMTAMEPIMFPELGPEAVFRIEVADFPLIISID
                     TRGAIFS"
     gene            complement(12958..13776)
                     /locus_tag="PL056_03445"
     CDS             complement(12958..13776)
                     /locus_tag="PL056_03445"
                     /inference="COORDINATES: protein motif:HMM:NF017493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HPM46106.1"
                     /translation="MRIIDGTHIIEAVRNLFIELNYQPESLKNISFKEEGMSDDEAEL
                     AKILDENMLNAKDELRPLCQDCGIAQVFIKKGKFVSYSDAPELSILINKGVELAYSDG
                     LLRKSTVRDPFERVNEGKNLPAFIHIEECEGDIFELWGLVKGGGSENVSALKMISPTE
                     GRTGVANFVLETLRSAAGKGCPPYFIGLGTGGTFDSIPVTAKKALLEKCNEDEELYDM
                     IMKRIRELDFGILGFPGISAVKELYIKKEPTHIAMMPVAVALNCHSFRSGKIIF"
     gene            complement(13891..15009)
                     /locus_tag="PL056_03450"
     CDS             complement(13891..15009)
                     /locus_tag="PL056_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020527077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM46107.1"
                     /translation="MYERSLHAVIAKKTGGNKAIVIVGPRQVGKTTLIQNFLKDREHL
                     FLDADDPTVRVLLTNPNTEELRRIIGKSTTVFIDEAQRVPGIGITLKIITDQFKSVQL
                     FVSGSSSFELSNELNEPLTGRKWEYELLPVSWEEYEHHHGYLVAEQQVENRLLYGFYP
                     DVLNHPGEEKDILKQLVNSYLYRDILAYANIRKPEILEKLVLALALQVGSEVNYNELA
                     QTVGVDRKTIENYVNILSQGYVVFSLGSYNKNLRSEIKKSRKIYFYDNGIRNMIIGNF
                     NSLDMRSDKGSLWENFLVSERKKQHAYKNSFSNSYFWRTRQQQEVDYVEESGGKLTGF
                     EFKWKAKSGLKLPKTFTETYTADTKIIDRSNFRDFVVM"
     gene            complement(15150..16559)
                     /locus_tag="PL056_03455"
     CDS             complement(15150..16559)
                     /locus_tag="PL056_03455"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HPM46108.1"
                     /translation="MKQGHLIFILFIFVTVSCTKEISIPVIVTTDIHGMFVSQENEPG
                     SLASIATAVNEIRSKNKDLILLDNGDILQGTPALHYFNFIAKDRKHIVSQVMNHLKYD
                     AASIGNHDIEAGFENCKRIEKEFTFPWLSGNITLNETKMPLFKPFTIIERKGVRIAVL
                     GMTTLTAQKAVRPENIEILYLNSIREMSEKWIRIIKQDHKADVIIGLFHEGLEFVKPV
                     AESVKGFDIIFTGHDHTVNKETVKSPEGKEVLIVGSSDSGKSFVLAEIIKSKGIFSFK
                     GDVVPIKKTSEDTGFIDFIEPLLQEAVKYESSVAGEAPVEITPEIYVDIIHETLLRIT
                     NAEVSLTAPVTDDVSLKAGNVTVRDLFYLYPFDNFPVTLKLSGKEIESLLKYSHELQK
                     PGSRYKRFYNDISPRYTAAIPLEQTRLYTVVMNSYHASDGGEILSKGIGLDAETIKSR
                     IVKIFPENIREHLFKTENR"
BASE COUNT         4653 a         3485 c         3487 g         5085 t
ORIGIN      
        1 ttcttgccac gaaccccggt cttttcagcg ggttgatgct ttctcagtac acagccgaca
       61 cactgatcgt tgaacagaga attctctgca cgcctgcagc aattcagtca attcctgccg
      121 ccgcagatca ggaggatttc gtttcaatgg gaatgaatac ggcaataaaa aaccgccaga
      181 tatatgaaaa tgcgtgcgga gttttaggaa tagaatttat ggcggcttcg caggcacttg
      241 atataagaaa atatgaaaat gaaggaaaag gggttgccgc ggcaaagaaa aagatccgcg
      301 aacatgttga attccttgaa attgacagac cgctctatcc cgatcacacc gaaatgcaga
      361 aacttgtcat gtcaggagaa attctcaaag cagttgaaga agagtgtgtt tttccgtctg
      421 atgagtaggt ttgttcagat ttgtgagtga tcgatcttgt tttcctcagc atccatttcg
      481 ctcagcactt tgttgaaagc tccgatgaag ggctgcatat ctttcatgag ctgggctttc
      541 ggattgaccc gcaggaccat tccgtcaaga tacatcatgc tttttatgat cgagaacata
      601 tccttttcaa accgcattcc gctgtgaacg gcaagtttaa tggtttccat catctttttt
      661 gtaagactta cttcgcttac tgtgcttcct gcaaaatctt tgtagagttc cataaatttg
      721 actttgtaac tgtctaattt ctttcctgaa attgtcgttg ttgccatgag ttgtattctt
      781 tcggcgctcg tttcatagtc aaactggcta agcgattcaa aaaaacggaa aagaccgacc
      841 cgcattcttt tggaaactga acttactgca cctgtatcaa tgaaatagaa atatccgtca
      901 tcatcaacca tgacatttcc cggatgaatg tcaccgtgaa aagtacctgc ggcaaaaaga
      961 tagaatccgt ggatctggaa aaggtcaagg atcatctgat atgtcacttt cccttcaaca
     1021 atgaggtcat caagcgtttt tccctttatt ctttcgctta caagataccg ctcaccggtc
     1081 atttcccggt agatcttatg gaacttcaac tttgagaaat cgtatctgtg acggttgtca
     1141 tcagcaagtt tcataagtgt ctgctgaccg ttatattcat caacaagatt aagttcttga
     1201 gttgtgtact gagcaatgta gtcaaggatt gcgaccggat caaagaccct ctcaagttta
     1261 ggataaaaga agatagctgt tttcatcaat cttttcagtc gcttaatgtc atataaaaag
     1321 ttctcaagat agtcgctttt tatcagtttt acgataactt cagtcccgtc atgaagcaag
     1381 gcattatgaa cctgtcccac tgaagcagca gtaaaagggt tgtagtcaag acgggcaaaa
     1441 ttcttctccc acccgtcagg agtcacttca cgcagaagag attcggaaac agttctatca
     1501 agctttgaag cgtgtccgta aagtttcatc agatggcggc agaccgattc atcaagaaaa
     1561 tctatcctta acgcaaaatg ctgggcaatt tttactgcaa gaagtcccat tttctcaatc
     1621 ttttcaagat ctggctcctt tttgccgtaa atctccctta aaagctgaac aaaatcaata
     1681 attttcatag catcactccc ttaaaataag ttcagccgag atccgtcctg attcataaat
     1741 tgtgggaagt ccgcttccgg gatgtgttcc tccacccaca agatagcagt tgccgatatc
     1801 ttcaaacttg ttgtgaggcc tgaaatagag catatactta agtttgtgac ttatgttgaa
     1861 tatcgccccg ctataaactg aataatcttt ttcccaatca gttggagtta ttatcacttc
     1921 ttcttcgatt ttttgtctaa gtccttgaaa cctctgattt tcaagcttgt caagaacctt
     1981 ttctcttaac ggcccttttt ctttttccca gtcaattccg ctagtgttgt tcggcatgaa
     2041 aacaaggaca aaaagagttg attttccatt tggagcaaca gttttatcaa caaccgaagc
     2101 gttctggaca taaattgaaa gatcgtcact gatcactttt cttttaacga tgtcatcaat
     2161 attgttgcgg taatcatcgg caaaaaggat gttgtgatga ggcagatcgt caaaaattcc
     2221 gtccacaccc agatacatga gaaatcctga gcagctgtat tcaagcgatg cgatctttct
     2281 gtcgttatat ttctttctgt gtctttcttc taccagatgt gtcatggcat gggcaaaatc
     2341 ggcgtttatg atgacaaaat cacttttgtc aatagtgccg ttttcaagca acactccttt
     2401 tgcagttcca tcttcaacaa taatttcaga tacttttgtg ttaaagtgga tttttccgcc
     2461 agactcttgc acgatatttg ccatcgcctg agaaatttta ttaagtccac cttcaacatg
     2521 aaatatccct ccaccatgct caacatacga caggatgctg aaaagacccg gagcttccca
     2581 aggtgacatt ccgatatatt ttccctgaaa agtgaaagct ttctgcatat attcgtcttt
     2641 gtaataactt ccaatgacat cgctaagcgt tttgagaggt gttgcacttg gacctgagga
     2701 aaggagttcc cagtcaagaa gatcggtgac actgttaaat ggctttctta atatcgggaa
     2761 tatttttctg tacttacgag cttcatcttt aagatatttc ttatagccct caaaacttcc
     2821 gggactgaac ttttcaattt cagatttcat cttttcatga tcgcggcttg ggaaaaactc
     2881 ttttttgccg ccatcaaaag agagtcggta catcggctca agttcagtaa tcttcacatg
     2941 atcagtcagt tttttccccg acattttaaa aacatcttca agaatattga gcatcatcag
     3001 aaatgtcggt ccaatatcaa aagtaaaccc gttccttgaa atgaagccgt tccgtccacc
     3061 cggataactt ttctgttcat ataccgttac atcgatccct tttgacgcaa ggatcattgc
     3121 agaactgaga ccacccggtc ccgcccctat gacaataact ttttttgaca ccttccctcc
     3181 ggaatatgaa ttattaacaa aaacagcgtc ttaatcttcg ccggctttct gtttttcgag
     3241 tttcttttcg atgtttttca aacggtcgcg gatcgttgga tagtatctgt tgtagcggtt
     3301 atccttcata gactggatgt agaaattttt cgcacggtcc cactgattga gcttttcgta
     3361 acatactcca agaagaagtt cagcccggtc tttctgataa tcctttttgg tgttgatgaa
     3421 aaggaaccgt tcaaatgcta ttgccgcctt gttgtaatcc ttcattctgt agtaggagag
     3481 ggatgtaaga tagaaaagat tgggtatgtg gtctcctgtc gaagaaattt caagcgattc
     3541 cttgaacctg tcaacagccg cctcatagct tttcctttta aaaagtattt caccttcatc
     3601 atatttctgg acggcggctt tctgttttat caaactggtc tctttgtcaa tgataagccg
     3661 ttcaagacgg ctgagcgctg aaagattgaa ttccccgtaa agtttgaaag cgtggtcggg
     3721 atcaccctct ttcagtgcaa gatagaggtt gtatgccttg atgtcgttat tttcaagtga
     3781 gaaaagcttt tctttcagtt cttcaacttc tttttcaaga tattcgttgt gttcctgcat
     3841 gagagtatat ttttcagcaa ctttgacaac ttccgccctg aagtagaaga aaaatgcaag
     3901 gatcatgact atcccaagga tgacaaaagt gatgtaaaac cggtttgcct caccttgatc
     3961 tttgcggtta accttttgac ttatttcttt aattgcagca tcgagcgcgt taagactgtt
     4021 tatggatttc atcaggagag tacggatctc ctttgaatcg tccatcggtt tagtttttcc
     4081 gttctccgtt gatacaacag aaatcttttt ttcttcggcc atcttaaaag ctccgcaata
     4141 aagcagacgg gagtttaacc cattttatgt taatttcaaa aaaaatagga aaaaccttta
     4201 aaatttggta tcaaatggtg tactttttca ttttggagac ttgtatgaca aaaggatttt
     4261 tcgaattcaa gccagtcgga ttcagaggtg aggttgcgat agttgctccg tcatcaccgt
     4321 ttgacagaac gcttttcaat gcgggaataa cagagctgaa acttaacaag ctgagacctg
     4381 tgatagacaa aaatgttttc ctgaaggaaa gttttcttgc cggaaacgac acatcaaggg
     4441 cggaactgct tataaattca ttttcaaagg aaagtgttga ggcggtctgg actgcaagag
     4501 gcggatacgg ttctataagg ttgcttgaga tacttgaaag aaatatcgaa aagataaagt
     4561 ccaatccgaa gctttttatc ggttttagcg atgttaccgc aattcattca tggcttgttg
     4621 aaaaatgcgg gtttgttact gttcacggcc ctaacataac gacactttca acggttgata
     4681 agtattcgaa aatacagata tttgagattc tttccggtgc tgaaagggct ttcagcattt
     4741 tcagcaagaa catcagggcg gtaaatcacg gcaggtctag aggaatggtc aaagggggaa
     4801 atctcacaac tgtcgcatca cttctgggaa caccgtttga gcccgatttt acgaactgca
     4861 tcatgttcct tgaagaagtg ggagaggttc cctaccgtgt tgaccggctt ataacgcagc
     4921 ttcggctcgc tggaaaattc aagggaatta aaggacttat tctcggagat ttttcttaca
     4981 gggaattcag gccgcctgct gaaaaaagtg taaatcccta tgcaataatc gaatcagccg
     5041 gaattgaaag gaatgttcct gcgatctgca acttcccttc aggacacggg aatcagaata
     5101 tggcattcct tcttggagcc atcgctgaac tcgacacgga atcaagaact ttgaaatata
     5161 ctttttcata agcggagcat gcggtttggg cagtaataaa aaaacaagag aggagatgat
     5221 agagcttgca tatgctctcc agaatgccgt tactgcactt tgcagcaaaa ggcctctacg
     5281 gataaccaca aataaagata acagcattct caggatagtc tggatgaagg gttacgagaa
     5341 aaaagagtgg tataatgcgg cgggaatgaa gatactgaca gctgctttca ctgataagca
     5401 tggaatacat gtcaaggaaa taacagttga cgaatgtaat cattccgcag tttatcttac
     5461 aaagaaaagc actgatccgg aaaggacggg aaggtaaatg aaaagggtat ttgttcccgt
     5521 atttattctt ttttttcttt tgtcagcaaa agcggattgt tacagcggtt tcatcccaaa
     5581 gatgcggctt tctcacagta actcaaagga atgtctctat aatccgctga tccatataaa
     5641 ttcagaagac agggttgcga tagattatct tgaaactgtt tacggagaac ctaaagattc
     5701 tttcactata cttcccaggg gattttacga tacagtgaaa tattcaaacg acccttacag
     5761 aataaagccg tttcttaacg gtgtcgaaac atcaaattac tggcagataa tttcaagtgc
     5821 aggtgtcgaa tattattatt caaacagagc agcgaacttt attcccggaa gttcaggaaa
     5881 aatatttcaa cacccgtcaa cctttgtgat gagccagtca gggaacatgg ttcttttaaa
     5941 ttcgctggca ctttactggg aactcaggga atacaattat tttgataatt cttcaaactg
     6001 ggaatcattc tcagtcgatt ttaacagagt ctatgcaaag ctcaagctct ggaaaatatc
     6061 ggccatgttc gggaaggacg cgcttcatct cggaccgggt gaacacggca tgatcctttc
     6121 agcaaacgcc gcgccttact ggatgatgaa atttcagaac gaggagacca tccgactttg
     6181 gggaagctgg aattttgtaa ttatgaaagg gtggctgaaa gatgaacggg aagatgtatc
     6241 aaatcctgaa atatttgcaa tgagaatgac atacagaccg caggggtcat tcgatttttt
     6301 tgagcttgga atgactagga caatgatgta ttcaggtgac ggcatgtacc actacaagat
     6361 agttgactat ccggtcctga tttcaggggc aaaggacaac ctgccacgcg gaaagtttga
     6421 tgccgacagt tacggcggaa ttgattttac attcaacatt ccgttttaca agctggttcc
     6481 ggagcttaaa gtcttcaagc tttatttcga ggagtcaggc acagacatta aagccatctg
     6541 gcaggttgaa gacctcggcg atttcaccat accttacata ctgttcaaat tttacgaaag
     6601 ggcttatctg acagggattt tcatagggct ggaaaatgat gcaatgcgtc tggaatatac
     6661 aaagactgca ttttcatttt ataagcacca tctttatccg cacgaaggat attcatacag
     6721 aggaatgtct ctcggtcatc cgctgggaaa caaccatcag gcattaaaat tcaaccatac
     6781 ccactggttt gaaaacggtt tatcatttaa atgggagatc ggttattatc agcttcccgg
     6841 aataaacaga aatgataaag acaaatcttt ctctgcactt ttccccatgt tctctctgga
     6901 tgaaggtctg ataaggagag gttatttctc actctgggcc ggctgggtgg tcaagggcca
     6961 tattttaagg ggatattttt ctcttgacgg cggaccaaaa agcgattccg atccacgccc
     7021 ttcaaagata actgtcagcg ataaagcgac cgttgacata acactgggtt tttcattcct
     7081 gttaaagttc taaattttat aacaaaaaaa gcttgacagt tggatgccgg catgtaaaat
     7141 gccacgcgtt atttgggtta agctctttat tattggagga gtgattatgt atgcgatgat
     7201 cgatttcggc ggcaaccagc ttaaagttgt ccccggagaa aaagtactgg tttacaattt
     7261 gaacaaaaaa gaaggtgaac ttgttgagaa tactaatgtt cttctttttt ctgacggtga
     7321 aaatgtttct atcggcaaac cgtatctcac agatgtgaag gtaaaacttg aggttgtaaa
     7381 gaacttcaaa ggaaaaaaag ttattgtttt taaaaagaga agaaggaaaa cgagtaaagt
     7441 taaaaacggt ttcagacaga gttttactca gcttaaggtt attgaaatag ttaaataaac
     7501 agggagatta gatcatggca cataagaaaa gcggcggaag tgcgaagaac ggtcgtgaca
     7561 gtgcaggtca gagacgcgga attaaaaagt tcggcggaga gccggtaatt gcagggaaca
     7621 tcatcgtccg tcaggtcgga acaaaatgga ttccgggaaa gaatgtcggg ctcggtacag
     7681 attacacgat ctttgcgctt tgtgacggtg tggttgaata tcagaccttc agaaagaatg
     7741 ggactgaaag aaagagagtt aatgttcttc ctctcgaggt ctgaagtttt caataaaata
     7801 agtttcaaac tcccgtaaaa gcgggagttt ttttttgctt ctttccggag atatcaaatg
     7861 agatttgttg atcaggcagt aataacggta accggcggta acggcggagc cggagcagtc
     7921 agtttcagaa gggaaaaatt cgttcctctc ggaggacctg acggcggtga cggcggaaaa
     7981 gggggcgatg tcattcttga gggtgatgag aacattacaa ctctttatga tgtaaggtat
     8041 cagaagaaca tcaaagcaga gagcggtgcc aacggcatgg gtgctaatat gtacggcaga
     8101 gggggaaaga acgaaactgt gaagatccct gtcggcacac ttgtttatga caattcaacc
     8161 ggagagctga tcgcagatgt tacaaaacac cgccagaaag ttgttgttgc ttcaggaggt
     8221 atcggcggca gaggtaatgt gaagttcaag acaagtgtaa ataaagcccc tagacatgcc
     8281 cagtcaggga tagcagggga atcaaagatc atacggcttg agcttaagct tatggctgat
     8341 gtcggcataa taggataccc ttcggtcggt aaatcaacgc tgataagtgt gataagcaat
     8401 gcaaggccta agatcgcgga atatcacttc acaacactca cacccaacct cggaatggtg
     8461 gaaggtcctg attttattcc gtatgttgtc gcagatattc ccgggcttat tgaaggtgcc
     8521 catgaaggac tcggactggg gctgcagttt ttaagacatg tggaaagatc gagattttta
     8581 attcacctcg tagaaataaa tcctgcaaga acaagtcctg tggatgatat aattacaata
     8641 aacagggaac ttgaactttt caattcagac ctggcgaaaa gggatcagat atatgtcctt
     8701 aataaaactg atatttacgg ttctgaacac gaagaattga gattggaact caagaaattt
     8761 gtagaaggga agacatattt tgagatcagt ggcgttacaa gagagggcgt taaagatctt
     8821 atggcttttg tcggaaggat ggtcgctgaa tataaattga aagagaaagt cgatcagtca
     8881 gagcttaaag aatcaatata gttcttaata gtttcgcgat cttcttgcga aagatttaaa
     8941 aaccttattc ccattccggg cattgatgaa tcttctgaac ttggaacaaa aatagaatat
     9001 ttgtcgtcac ttcttgcaac cttgcctttg actttgattt ctttttcgca attgggaagg
     9061 aagaaagaaa ggtcaaattc ctgttcaggt tcccacagaa tggatgattt tacgaaaagt
     9121 cccccttcac tgagattgtg cgtgtcaaat ttgatgctta tgtcatctcc gctttcagtc
     9181 agtctgatct tgagagatat caaagccctt ctgaagtttc tgttgtctct acccatcatt
     9241 gttttaatcc ctcttaaacc ggtttgttgt cggaatctac aaaaaccagt ttcggttcag
     9301 gtatttgaga agcatcgtca atatatgaat gactggcgat tattaccaga tcgttctttg
     9361 caacaagccg tgctgcggca ccattgatgc agatctcacc ggacccttct ttgccctcga
     9421 tcacataagt ttcaaacctt gcgccattgc tgatgtccca aatgtcaaca aattcaaatg
     9481 gtttgaggtc tgcaagctcg ataagattct tgtcgatagt gatacttccc tgatattcta
     9541 tgtcagctcc ggttatcctt gcacgatgta tttttgattt taatattttt ctaaacacaa
     9601 aatcctcaga aatgaaacat ccaaaaacag tcagaataat agcaaaagag ttgatgttgt
     9661 caagggttga atttaattac cgctattttg agcgagctct ctgcctatcg caacggcttc
     9721 gagattgaat ttttcggttc ctttcggggc tgattccaga accgcttttt ccagtgattc
     9781 gaagcttacg atctttgtta tgcctacaag cgccgacaaa gaaacgacat taagggcaat
     9841 aagttttccc tgtttttcaa gagttttcga tgacatgggg agttttatca gtttatagtt
     9901 ctcacattca ggaagatcga cataatcgct gtcaaggatt acaaccccac cttttttcat
     9961 tgttgaaata tatttgttgg ctgctttctg agtgaggcac accattgcat caatttcacg
    10021 ggctttcgga tatttgaccg gattgtcgct gatcacaaca tcggcctttg atgctccgcc
    10081 tcttgcctca ggtccgtatg cctggctctg aacagcatgc ttcttttcaa aaagtgctgc
    10141 ggccgttccg agaatgacag atgcaaggat gacaccctgt ccgccgcttc ctgaaaatct
    10201 tacttcatat ttcattttgc acctcctgaa acggagtcaa taattttctg atatttgtca
    10261 gtaaattcaa gcttttcttt gtcacagaat actcctgtta cgatctttcc tgcgagttct
    10321 tcttcagaca ttccgccggc tttttcaatg gaaatcgcat tatctttctg atatttgagc
    10381 atatcgaggt tcgatccgta tttcttcttg ttttttctgc cgtgagtcgt gtagcatgct
    10441 tcagtaacat caataactga aaaacccttg tgcatcaagc ccttatgaat gtatgtatca
    10501 agctgcggaa catggtagct tgtccctctt gcgacaaaag tagctcctgc acctgtggca
    10561 agatcaacga catcaaaatg aggttcaaca ttgccgtaag gagatgtcgc agttttatct
    10621 ccgtcagcag tggtcggtga gtactgtcca ccggtcatac cgtagatgcc gttgttgaaa
    10681 acgatgagtg ttatgtcaat gttccttctg cacgcatgga tgaaatggtt gccgccgatg
    10741 gccagagcgt caccgtcgcc tgaaacgacg atcacatgca ttttaggttt tgcaagtttg
    10801 atccctgttg caaaagcaag tgctcttccg tgggctgtat gaagggtgtt gcagtccaca
    10861 taaccgggga gacgggatgc acagccgata ccgctgacta aaacgatctc gtctttgctc
    10921 cagccggttt tgtcgatagc cctgaggagt gatttcatta ctatgccgtg tccgcatccg
    10981 ggacaccaga tgtgcggaag cttttcgttc cttaaatatt ttgaataatc gaacatattt
    11041 tatctcctct cttttaaata tttaaatggc tgatggctga aattatggtc tccggtccga
    11101 gcggttctcc gttgacaagg ttcacaggga ttatcttgtt tctttttgtg aatctttccg
    11161 caacggtttt aagctgcccg aggttcattt cgggaacgac aataaaatca gcgttcagag
    11221 cgtattttgc aaactcttct ttcgggaagg gccagagagt aataggtctg aaaagtccgg
    11281 ctttaacacc tttctttcta agctccaacg ctgattcttc agcagctctt gcagcacttc
    11341 cgactgcaag catgagtatt tttgcatctt cacagtgtat cgcttcaaac ttatcaacaa
    11401 ggtttctttg agtataaacc tttcttatta tcctttcatt ttctcttgcc acgatctcag
    11461 ggtccattgt cgggaaaccg gaatcatcgt gattaagacc tgtcacatgg aatctgcttt
    11521 ttccgaaatt gggcagacat gcgggtttat atccgtcaga agcataggga aggggacaga
    11581 gtccgtcact gttcggtaca gttctgttaa ttatttcaat ttcaccggga tccggaattc
    11641 ttatcctttc tctcatgtga ccgattattt catcacccag aacgattacc ggggttctga
    11701 aatattctgc aagattgaaa gcccttacag tttcagtgaa catctcttca actgaattcg
    11761 gaacaagtgc gatcgtagga tggtcaccgt gtgttcccca ttttgcctga agaaggtcag
    11821 actgtgccgg agaagtcgga agacctgtcg aaggcccgcc gcgcatgata ttaacaacaa
    11881 cgatcggaac ctctgcgata cagccgtatc cgatcagttc ctgcatgagc gagaatccgg
    11941 gtccgctggt ggaagtcatt gattttgaac cggtaagtga cgctccgatg atcgagccga
    12001 ttgaggcgat ctcatcttcc atctggatga acttgccgcc gtttttcggg agccactcag
    12061 ccataaattc agcaatttca gtagaaggtg ttatcgggta tccgccgaaa aaactgcaac
    12121 cggcgtatgc ggcccctttt gcacaggcaa tattaccctg aatgaattca tagttttctt
    12181 ttgtcatttc tttgctcctt cttttttctc gatataaatg gcaaaatcgg gacatctgag
    12241 ctcgcacatt ccgcaggcga tacatttttc catgttgtct gccgtcacaa tgagtttctt
    12301 cttgtcaagt gaaagagctt tcaccggaca gaattcaata cacacttcgc atcctttaca
    12361 aagttcaggt ctgatgtaca gtgtacattt ggcgttttca tacttgattt cgacaaattc
    12421 tttttttccc atgatcacct cttaagaaaa tattgcaccc ctggtgtcga ttgatattat
    12481 cagcggaaaa tccgcgactt ctattctgaa tactgcttcc ggtccaagtt cagggaacat
    12541 tatcggttcc attgccgtca tccggcttcc gtaatatgcg cctgctccgc caagtgcctg
    12601 aaaataaagg gctttgtgtc ttcttatcgc ttcaggtaca ccgagaggaa gaaacccttt
    12661 tccgataaat ccctttactc cgagttcgag gaggaacggg atgaaagcgc ccattctcca
    12721 gcttgttgtc ggtccgatgc tcgccctgtg ttctgtataa ccgggagtcg gagcggcaaa
    12781 aaatatgtat gcccctttca tttcaaatgg aagcggctcg cctttttcaa ccatttcgat
    12841 gatctttttg agtgactggt cacgcacggt tatgagttct ccggtaatac gggtcaccat
    12901 tccggcgttt agtgcttctt cagacattcc gttagaaaat atcaaaaagg tagctcctta
    12961 aaaaattatt tttccgcttc tgaaactgtg acagttaagt gcaacagcaa caggcatcat
    13021 cgcaatgtgg gtcggttcct tttttatgta aagttcctta actgccgaaa ttccggggaa
    13081 tccgagtatt ccgaaatcaa gttctctgat ccttttcatg atcatgtcat ataactcttc
    13141 atcctcgttg catttttcaa gaagtgcttt ctttgctgta accgggatgc tgtcaaaagt
    13201 tccgcctgtt cccaatccga tgaagtaggg cgggcatcct ttccctgccg cagatcgcag
    13261 tgtttcaaga acgaaattcg caacaccggt gcgtccttca gtcggggata tcatttttag
    13321 cgcgctgaca ttttcgcttc cgcccccttt tacaagtccc cagagttcaa agatatctcc
    13381 ttcgcattct tcaatgtgga tgaacgcagg aaggtttttt ccttcgttta ctctttcaaa
    13441 agggtcccgt actgtcgatt ttctaagcaa tccgtcgctg tatgcaagct cgacaccttt
    13501 gttgatgagt atcgaaagtt ccggtgcatc gctgtatgaa acgaactttc ctttttttat
    13561 gaagacctgt gcaattccgc agtcctgaca gagcggacgg agttcatctt ttgcgttaag
    13621 catgttttca tcaaggattt tggcaagttc cgcttcgtca tcagacatcc cttcttcttt
    13681 aaatgaaatg tttttcaaag attcgggctg atagttaagc tcgatgaaga ggtttctgac
    13741 cgcctcaatt atgtgagtcc cgtcaataat tctcattgcg accccaaata actaagggat
    13801 tattaaatat tgatactgta taagtcaagc aaaatcacaa aaagtttatg tttgacctta
    13861 tattcttgtc aaatattact tcctgaaata tcacataact acaaaatccc taaaattact
    13921 tctatcaata attttggtat cagcggtgta tgtttcagta aaagttttcg gcagcttcag
    13981 tccggacttt gccttccatt tgaactcgaa tcctgttaac ttgccgcctg attcctcaac
    14041 ataatcaact tcctgctgtt gacgggtacg ccagaaatag ctgtttgaaa aactattttt
    14101 atatgcatgt tgtttttttc gttcagatac gagaaaattt tcccatagag accctttgtc
    14161 agaacgcata tcaagggaat tgaaatttcc gatgatcata tttcgtattc cgttatcata
    14221 aaaatagatc ttccggctct tttttatttc actcctcaag tttttattgt aacttcccaa
    14281 tgaaaaaaca acatacccct gactgagaat gtttacataa ttttcaatag tttttctatc
    14341 aacacctacc gtttgagcaa gttcattata attaacttcg cttcccacct gcagggcaag
    14401 tgccaaaacc agtttttcaa gtatttccgg ttttctgata ttggcataag ccagaatatc
    14461 ccgataaaga taactgttaa ccaactgttt taaaatatcc ttttcttctc ccggatgatt
    14521 taatacatca ggataaaatc cataaagcag acggttttca acctgttgtt cagcaacaag
    14581 atatccgtgg tggtgttcat actcttccca gcttaccgga agaagctcat attcccattt
    14641 tcttcccgtc aacggctcat ttaattcatt tgaaagctca aaagatgatg acccgcttac
    14701 aaaaagctgc accgatttga actgatcggt tattattttc agtgtgatgc cgattcccgg
    14761 cactcgttgt gcctcatcaa taaaaactgt tgtggatttt ccgataattc tccgtaactc
    14821 ttcagtatta ggattggtta aaagcactct gactgtcgga tcatcagcat ctaaaaacag
    14881 atgttctcta tcttttagaa aattctgaat aagagttgtt tttccaactt gacgaggacc
    14941 aacaataaca atcgctttgt tgccacctgt tttcttagca attacagcat gtaatgatct
    15001 ttcatacata aaatcctcca taaaaaaaca cagaaatcag ggaacatatt cccatatagt
    15061 aataactatt tgggattaca ataccatatt gttatactct tctgtgataa atatgcaaga
    15121 aaactgtcgc aatggttttc tccgtctttt caacgatttt cagttttgaa aaggtgttcc
    15181 ctgatatttt cggggaatat tttaactatt cgggatttga tggtttcggc gtcgagtccg
    15241 atcccttttg acagtatttc acctccgtcg gatgcatggt aagagttcat gacaacggtg
    15301 tagagacggg tttgttcaag aggtattgca gcggtgtatc ttggcgaaat gtcgttgtag
    15361 aaccttttat acctgcttcc gggtttctgg agttcatggg agtatttaag aagcgattcg
    15421 atctcttttc ctgaaagttt caatgttaca ggaaaattgt caaaaggata cagataaaaa
    15481 aggtcacgga cggtcacatt gcccgctttt aaagaaacat cgtcagttac aggtgctgtg
    15541 agcgaaactt cagcatttgt tattctaaga agcgtttcgt gtatgatatc gacatatatt
    15601 tcaggtgtta tttcaaccgg tgcttcaccg gcaaccgaac tttcatattt taccgcctct
    15661 tgaagcagcg gctcaataaa atcaataaat ccggtgtctt cagaagtttt tttgatcgga
    15721 acaacatcgc ctttgaatga aaaaattccc tttgacttga taatttccgc aagaacgaac
    15781 gatttgccgc tgtccgatga cccgacgatc agaacttcct tcccttcagg tgatttaaca
    15841 gtttctttat tcacagtgtg gtcgtgtcct gtgaaaatga tgtcaaatcc tttaacactc
    15901 tctgcaacgg gtttaacgaa ctcaagtcct tcatggaaaa gcccgataat tacatcagct
    15961 ttatgatcct gtttaattat tctgatccat ttctccgaca tttccctgat actgttcaga
    16021 tagaggattt caatattttc aggccgcacc gctttctgag cagtcagcgt tgtcattccc
    16081 agaaccgcta tgcgaacccc ttttctttca atgatcgtga aaggtttaaa cagaggcatt
    16141 ttggtttcat taagcgtgat attcccgcta agccacggga aagtgaattc cttttctatt
    16201 cttttacagt tttcaaaacc ggcttctata tcgtgattcc cgatcgatgc ggcatcatat
    16261 ttaagatggt tcattacttg agaaacaatg tgttttctgt cttttgcgat gaagttgaaa
    16321 tagtgaagag ccggtgttcc ctgaaggatg tcaccgttgt caagaagtat cagatctttg
    16381 tttttactgc gtatttcgtt cacagctgtt gctatcgatg caagagatcc gggttcattt
    16441 tcctgtgaaa cgaacattcc gtgaatgtct gttgtgacaa ttacgggaat tgagatctct
    16501 tttgtacagg aaactgtcac gaaaataaaa agaataaaaa tcaagtgtcc ctgtttcaaa
    16561 acatcctcca tcattataag accgggtaaa tttaacatga agagaaaaat atggcaagaa
    16621 agaaagagtt gtatataatc agtatgtaaa ttttcttttg gagtcaacta taaataaatg
    16681 atttcatgca gcgacataaa attcctcctt
//
LOCUS       DAOOYB010000035        16645 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_3392_length_16645_cov_15.236709, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000035 DAOOYB010000000
VERSION     DAOOYB010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 16645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16645
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3392_length_16645_cov_15.236709"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(87..1016)
                     /locus_tag="PL056_03460"
     CDS             complement(87..1016)
                     /locus_tag="PL056_03460"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46109.1"
                     /translation="MAINILIFVVSFLAVIKGSTLATRYASRLAESFNISKYIVGFII
                     VAFISILPETLISINSAISDIPEFGLGTLFGSNIADLSLIFAILIIYAGRGMKIESKV
                     LKNVRLYPFFLLLPLLFGLNGYYSRIEGLFLIIAGIIFYYLVFKNGIDVSANLRNGNG
                     KYKSFLFLIISMVLLLIGSHFTVTSATALAHALKITPILIGMLVVSLGTTMPELFFSL
                     KAVKRKDDGLAVGDIMGSVLADATIVVGILATISPFFFPVKIVYITGVFMAISSFILL
                     KFMKTGKLITKNEGYLLLLFWVVYVVVEFLANR"
     gene            complement(1021..1677)
                     /locus_tag="PL056_03465"
     CDS             complement(1021..1677)
                     /locus_tag="PL056_03465"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPM46110.1"
                     /translation="MNNMITSNKISKTIRFLILSDFFLFFAVGLLAPIFAVFILENIE
                     NKIEVIGYAMSCYWMTRVIIVIPLSRLMDKIRGEVDEYTFMIVGTFLISIIPLFYTIS
                     SEPWHIYVLQIMNGIANSMAVPAWRILFTNHVDKKYIGFEWSLEDVGIGIATASSATI
                     GAFIASKFGFNVLFGIISFFGLISVLILIILSKKKRMAFKNLIYNKSERAPLKLDTFK
                     "
     gene            complement(1670..2062)
                     /gene="rnk"
                     /locus_tag="PL056_03470"
     CDS             complement(1670..2062)
                     /gene="rnk"
                     /locus_tag="PL056_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013842521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HPM46111.1"
                     /translation="MNIYITKNDHKKLIKLINEKLPNDDYTHTLLLELKKATIVESEK
                     IPADVITMNSLVNFEDIESGAELEYWLVFPQDADISQHKLSVVSPIGCALLGYKVGDI
                     INLKTPGGERKIKVVKILHQPEAEGNYE"
     gene            complement(2299..3150)
                     /locus_tag="PL056_03475"
     CDS             complement(2299..3150)
                     /locus_tag="PL056_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006887321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HPM46112.1"
                     /translation="MFKISHKKNNKFHYSPLRYPGGKTFLFPFFDKVIKDNKLDGITY
                     VEPFAGGAGAALALLFLEKVDHIVINDLDKAIYSFWKSAIFNSEKFIKKINSTPINIK
                     EWRKQKTIYHDAKAKQLDRGFATFYLNRTNVSGILDGGPIGGVKQLGKYKINARFNKE
                     KLIEKISQLSQYKNRITVTNKDGLDLITEYLNKKNAFIYLDPPYYEKGSTLYLNHYKK
                     DDHQSLAEKLNKSPDAFWLLTYDNKKEIKSLYPDRRIINFSLNYNAYESRKGKEVMIL
                     SDVLNIK"
     gene            complement(3131..4537)
                     /locus_tag="PL056_03480"
     CDS             complement(3131..4537)
                     /locus_tag="PL056_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46113.1"
                     /translation="MKKEIAKPNYEEWPKQKIKVTDLFLDTKNIRLDIGVDLSQDALI
                     NDLFTNEDAMQVLRSIAINGFFPDEIPVVVKENSKYIVLEGNRRVAAVKALLRPEIVP
                     GKESAIKGILKTAIPVANEREVVIAPDRDSAMVFLANKHTQNTKRRWRPLRQAYFYKA
                     ELERGKTVQNLRDDYPTVDISKFLRLINIHKIAKSITFDSDSTAKKVHNEKTFPASTI
                     ERLYDDKQVRDFLGFDFDGNGEVKINIDKKEFEKGFKKIVEDVVEKNVDSRALNSEKD
                     RKEYLSNFVKENTPDKSKTGKVTTSKDFTEALIVTTKKRNRLAPSSISFTLQCGGVKR
                     MLKELQAIDYHKFPNAAHDLLRSFLECGLKAYFDHCKKTIAPKNGGKYVFLDTVLEEF
                     IKEMNQSKNNKLSQVASRIKDNSKMNSYSATFLNATNHNPDIFVTPQEVEDAWDAMEP
                     LFNYILAPNKKPNVQNKP"
     gene            complement(4586..7669)
                     /locus_tag="PL056_03485"
     CDS             complement(4586..7669)
                     /locus_tag="PL056_03485"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006670411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HPM46114.1"
                     /translation="MTNYKYTESDLENATLEWLEELGFGIVFGPNIAPDGESPERESY
                     KDIVLIGRLRSAVSAINPNIPRDAQEDAIKKVVNVAYSNPSLLETNHSFHRMLAEGVD
                     VEYRREDGSIAGDKVYLVDHKNLSNNDWLAVNQYTVIENNHNRRPDVIIFINGLPLAV
                     FELKNPADVNATVRNAFNQIQTYKSDIPSLFTYNAFCIISDGIDSRIGSLTADMERFM
                     KWRTIDGETVAPASIPEMEVLIKGVFTKDHLLDIAKNFIVFETDGETTIKKLAAYHQY
                     HATNKAIIRTIGATSEKGDRRAGVVWHTQGSGKSLTMVFYAGKIIQVLDNPTLVILTD
                     RNDLDDQLFGVFSNCHELLRQTPTQAKDRADLKKLLSVASGGVIFTTIQKFLPENKDE
                     KHPLLSDRRNIVVIADEAHRSQYDFIDGFARHMHDALPSASFIGFTGTPIELSDKNTR
                     AVFGEYIDVYDISRAVEDHATVPIYYEARLAKIQLSESEKPHIDTEFEELTEGEEVAK
                     KEKLKSKWARLEAMVGSDKRIKLIAKDVVDHFEARRDAMEGKGMFVSMSRRIAVELYD
                     EIIKLRPQWHSDDINKGFIKVVMTGSASDPANFQKHIHTKDERELIAKRMKDPKNDLK
                     FVIVRDMWLTGFDVPVLHTMYIDKPMQGHGLMQAIARVNRIYKDKQGGLIVDYLGIAP
                     SLKEALSYYADNDKEAPTIPQEEAVSVMLEKYEVVKALYHQFDYSSYFSSKASEKTRV
                     ISGAMDYVLSLDKGKDRYLKAVTELSHAFSLAVPSDESIQIRDDVAFFQAVGAVIRKF
                     DQNGGQGGPTEEDYDQAIKQIVSNAVVSDEVVNVFDAAGLKSPNIAVLSDEFLEEVRN
                     LEHKNIALELLKKLLNDEIRTMQRKYLVKSRSFVKMLEETIKKYQNQTIEAAQVIAEL
                     VELAKKIREEKERGNELNLTEDEIAFYDALCESDSAVMEIGDDTLKQIAQELVAMLRK
                     NTTIDWTLKEQVRAKLRVYVKKLLNKYGYPPDKQEQATKTVLEQAEILCKDWGNE"
     gene            complement(7675..8304)
                     /locus_tag="PL056_03490"
     CDS             complement(7675..8304)
                     /locus_tag="PL056_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46115.1"
                     /translation="MEHQTNLDKILYKLETSKEPFIKVTKEMLESANGNLYPLDLFVL
                     GIVKRSILLTSGFCSLIRQNNFLSAGPIVRLHLDNLLNLHASFIAPDPHEFSMSKLQG
                     ERTSKLKDKTGQFMTDTYLAESLSKEKETEWVINLYRESSKFIHVSDKHIFSTVEELK
                     KDGQMSFVISDKQNIPEKSIIEATEAMVSISEQIFRHLFGWIQAKNNKS"
     gene            complement(8309..9532)
                     /locus_tag="PL056_03495"
     CDS             complement(8309..9532)
                     /locus_tag="PL056_03495"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HPM46116.1"
                     /translation="MEIATKYKDTKIGTVPNEWGVFAVNDLCKVGRGASPRPVGDPKY
                     FGGSTPWIKIADANRTGTRYIEETRETVTEEGKNRSVFLKKGSLIVSNSGSAGIPKFL
                     AVDGCIHDGWLYFTEYKNLDDKFFYYWWLYNGQRVSDTYANGAVLQNLNTDLVRNLFI
                     ATPKIEEQKKISSILWNIDDKIELNRKINNNLETLVGIIFKQWFIDVKDNLPSGWKIG
                     KVSDVVKVESGFPFNSRMFDDFGKYKLVTIKNVQDGYFITECTDSLSEIPQKMPTHCK
                     LKDGDILLSLTGNVGRVCLVNGSNYLLNQRVANLIPIIENNRAFTYFLFRQKDFQNTL
                     MSISRGTAQQNLSPIETKSLEIVVPSQEALDRFSKIASPILKMLIENHNEIRNLLILR
                     DSLLPRLLNGKIVVK"
     gene            complement(9539..11092)
                     /locus_tag="PL056_03500"
     CDS             complement(9539..11092)
                     /locus_tag="PL056_03500"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HPM46117.1"
                     /translation="MAKQAKKASSANIGFEETLWSAADKMRNNMDPAEYKHVVLGLIF
                     LKYISDSFEEVYNEIKSDKDYYAGQEEDKDAYLAEGVFWVPQEARWEYLKANAKKPEI
                     GKMLDDAMIAIEKDNPSLKGVLAKNYARPDLDKVRLGELIDLMSSIGLGDKASRSKDV
                     LGRVYEYFLGRFAGAEGKGGGEFFTPQSVVKLLVEMIEPFKGRVYDPCCGSGGMFVQS
                     EKFVEAHGGQTHDLAIYGQESNPTTWRLCKMNLAIRGIEGNLGQHNADTFHNDLHKDL
                     KADFILANPPFNISDWGGERLKDDVRWKYGTPSAGNANYAWIQHMIHHLAPTGIAGFV
                     LANGSLSSNTSGEGEIRKSLIQNGLVDCIVALPSQLFYTTQIPACLWFVSRDRYDHKF
                     RNRHDEILFIDARKMGTMVTRKNRELTDEDLNKIIGVYHAWRTINGKYEDVKGFCKAA
                     KIEEVEKNGFVLTPGRYVGSDFVEEDDSSFEDKMTRVTANLSDQFKKSKEIENQIKHD
                     LKEIGFEIK"
     gene            11215..11787
                     /locus_tag="PL056_03505"
     CDS             11215..11787
                     /locus_tag="PL056_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46118.1"
                     /translation="MDSLIAKLYQSPKTILSNKDLALIWQENDKFNLNAKIAYYVKQK
                     ALIRLTRGVFAKSKDYNSKELATSIYTPSYISFETVLREAGVIFQHYETIFVASKWPK
                     TTKIDKYTITFRKLKDAVLFNSTGIVSKDNYSVATTERAFLDMIYLFPNYYFDNLKSI
                     NWEKCNEIAKIYNNKQLIKRLNKYRKNYAQ"
     gene            11819..12490
                     /locus_tag="PL056_03510"
     CDS             11819..12490
                     /locus_tag="PL056_03510"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HPM46119.1"
                     /translation="MKDIYTNVSISPLLGFKGGTCAYFFYNLPRFSVDLDFDLLVVNE
                     ENQKLVFEKIVAILSKYGQIKDQYIKRFTVFALLSYGDDDHNIKVEINVRKLVENIQD
                     HYEMKEYLGISMFVAKKDYLFASKLSALTLRNETAMRDIYDIHYFAKSNWDINTEVVK
                     ERTSKTVKEYLADCIAFIEKVKDSQIMQGLGELIDSEKEKAWVRNHLKADTIFMLKNY
                     MSVIK"
     gene            12497..13015
                     /locus_tag="PL056_03515"
     CDS             12497..13015
                     /locus_tag="PL056_03515"
                     /inference="COORDINATES: protein motif:HMM:NF015935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HPM46120.1"
                     /translation="MAKKEQKKHTPEPEKSDKRYLKKFEIVETDVELPIQGQVRDPED
                     LYKFLKDLENESVSKVIGIYLDDKNLFLGHQVFLGSSPKAFDTQLLYHYYALFLAKKF
                     IVLINHPGSTDPTPDEADKKLIRALQADAQVLSFKPFFADFIIVAKRSYFSMSTNDGT
                     ACHCGHQEYIPE"
     gene            13045..13218
                     /locus_tag="PL056_03520"
     CDS             13045..13218
                     /locus_tag="PL056_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HPM46121.1"
                     /translation="MNKIALGVSFGFTGGAIFGIILGLLTKDFAVWLAVGVGFGMIIG
                     SSVGLFLKNTKRN"
     gene            13550..14569
                     /locus_tag="PL056_03525"
     CDS             13550..14569
                     /locus_tag="PL056_03525"
                     /inference="COORDINATES: protein motif:HMM:NF014177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HPM46122.1"
                     /translation="MFSKFQALPSYFGGKRKLVKHIFKPIKKPDGIFIDAFLGGGSVS
                     LYAKAKGYKVISNDIAFRSVIIGRAIIANNSVKLESEDLARLFFETKHDSFIKKNYCP
                     KVFTTKIADFLDNAFASANKVENDTKRYLLLHLLIKFILSCRQFGKFTHTRDTLDLEA
                     RKWEWPLRSKSHAPKNLRMIQNPMMTLEKLKDEINHGIFDNHQENEIHQKDVFDFLKD
                     VRGDVVYFDPPYPGSSAYEVEYGILDNILANKKLDNKPSDFSNKDADEFLEKMFESAK
                     HIPQWIISLGQNKEDVGVKPEKLLAMVQKHRKAEVQMLDHKWSVANAGGRNQSNNIEY
                     MIVTL"
     gene            14566..15360
                     /locus_tag="PL056_03530"
     CDS             14566..15360
                     /locus_tag="PL056_03530"
                     /inference="COORDINATES: protein motif:HMM:NF014273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HPM46123.1"
                     /translation="MKKEKTEIKRVKIKECVPNWWNPNEMNARQFTSLKKALTKYGQT
                     KPIQVGVWPNMKAKAGYVIVGGYHTWKVLGELGETEIEANIHNFKNEDEAKLYGLTDN
                     IHGSNQQIKLGKLAYELTQNGYSIKDIAQSLGEEDINVKDALDLVKDEIEKKMIELKK
                     AERENTVVLNFIVDKDPEASADEFIKAVTAFAKSKGVKVEKAVKDINSKRETIALVSF
                     NVTNPQKGVIDEALNILSKQSKMTESEAIAEVCSQFINTLNGSKPQ"
     gene            15372..15794
                     /locus_tag="PL056_03535"
     CDS             15372..15794
                     /locus_tag="PL056_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46124.1"
                     /translation="MSKKVDIKSIRYQEAIRLKYNNESYSSIAKKIKVPVSTIEKWFA
                     FGGLLKEEYEKYRDEQNQIRHENGLEVFKKNVETASSMIVALMASENDTVKLNASKEI
                     VYRQYGKPKETVEHKGLFESGLNYEQILRKSRDKPPDG"
     gene            15748..>16645
                     /locus_tag="PL056_03540"
     CDS             15748..>16645
                     /locus_tag="PL056_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46125.1"
                     /translation="MNRYYENQETNHPMVNNLIAFLRLCEADPKVFIEECLSIKTKDQ
                     KTVLLKFNTAQSMIYERIKMLRAKKKPIRMIILKARQEGVSTFCEGLIFEKTVRQENT
                     NSLIVAHEPESTDAIFAMSKLFYDMLPVDVKPMRRYDNKKQMVFENPDQKARADDPGL
                     RSRMVISTADKVKIGRGLTLHNFHGSEVAFWKNAKELMLSVMQAIPSLPTTAVFLEST
                     ANGFGGDGEYFYQMVQDALAGKNEFELIFLPWHLMPEYSQPFASEAERKQFAETLNEY
                     EKELLNKENLRYEQLKWRRWAIQ"
BASE COUNT         5195 a         3296 c         3101 g         5053 t
ORIGIN      
        1 agtgagaata aaaagcaaaa ggagcccttt tgagcccctt ttttgccgtt tgtttaccct
       61 tgtggcgagc tttctaaata cctttgttat ctattagcta aaaattcaac aacaacataa
      121 acaacccaaa aaagtaagag caaatacccc tcatttttcg taatcaactt tcctgttttc
      181 atgaacttaa gcagtatgaa tgaggatatg gccataaata cacctgtaat ataaacaatt
      241 ttgactggaa agaaaaacgg actgattgta gccaaaatac caaccacgat agtagcatct
      301 gccaaaactg atcccataat atcgccaact gccaatccgt catcttttct tttgacggcc
      361 tttaaagaga aaaatagttc tggcatggtc gtacctaaac ttacaactaa catgccgatt
      421 aaaataggtg ttatttttag agcatgagct aaggcagtag ctgaagttac agtaaaatga
      481 gagccaatca atagtagaac cattgagata attaaaaaca agaagctttt atattttcca
      541 ttaccatttc gcaaattagc agatacatca attccgtttt taaataccag ataataaaaa
      601 ataatacctg cgataattaa aaataagcct tcaattcttg aataataccc gtttaaaccg
      661 aatagtaatg gtaaaagcag aaagaaagga tatagtcgta cattttttaa aaccttgctt
      721 tctattttca tacctcggcc tgcatatatg attagaattg caaatatcaa agataaatca
      781 gcaatatttg agccaaataa tgtaccaagt ccgaattcag ggatatcgct aatggcagaa
      841 ttaattgaaa ttaatgtctc tggtaggatt gaaataaacg caacgataat aaatcccaca
      901 atatattttg agatattaaa actctccgct aaacgagaag cgtatcttgt cgccaaagtc
      961 gatcctttta tgacggctaa aaatgaaacg acaaagatta agatgtttat tgccataaag
     1021 ttatttaaag gtgtccagtt ttagaggagc cctttcagat ttgttgtaaa tcaagttttt
     1081 gaaagccatt ctctttttct tgcttaatat gatgagtatt aatacgctta tcagtccgaa
     1141 aaacgaaata atgccgaaca aaacattaaa accaaatttg ctggcaataa aagcaccaat
     1201 tgtcgcgctg gaagccgtcg ctataccgat cccgacatct tccagcgacc attcgaaacc
     1261 tatatatttc ttatcgacat gattggtgaa aagaatccgc cacgcaggaa ccgccatcga
     1321 gtttgcaatc ccgttcataa tttgcaatac atatatgtgc caaggttctg acgatattgt
     1381 atagaaaagc ggaataatag aaattaaaaa agttccaact atcataaaag tatattcgtc
     1441 aacttcaccc cttatcttat ccatcaatcg actaagcgga ataacaataa ttacccgagt
     1501 catccagtag cacgacatgg cgtatccaat tacctctatt ttattctcaa tattttctaa
     1561 aataaaaaca gcgaatatcg gtgccaataa tccgactgca aaaaaaagaa aaaagtctga
     1621 taatattaaa aagcgaattg ttttgctaat tttattgctg gttatcatat tattcatagt
     1681 taccttcggc ttcgggttgg tgcaaaattt ttaccacctt tatttttctt tcgccacccg
     1741 gagttttaag attaataata tcgccgactt tatatcctaa caacgcacaa ccaatcggag
     1801 agactacgga tagtttgtgc tgactaatgt cagcgtcttg tggaaatacc aaccaatatt
     1861 ccaattcggc accggattca atatcttcaa aatttactaa tgagttcata gtaataacat
     1921 cagccggtat tttttccgac tcaactatgg tcgctttttt caattctaaa agcaaagtat
     1981 gagtgtaatc atcatttggt aatttttcat tgatcagttt aatcaatttt ttgtgatcat
     2041 tttttgtgat atagatattc atggcgattt caatttaaaa aaacaaccgt tttgatcggt
     2101 tgtttttagt taacacacta aaacaaccga ctaagtgacg gtaggaaaag aaagtgatgt
     2161 atttttgtat atattgcgca gaaacataat tagtataatt ataacaaata taacgataaa
     2221 aaacaatttt ttctttaaat tcgctaaaat atatgaattt atttatttca ttatacaatg
     2281 ttcggttaaa tacaattttt atttaatatt taatacatct gacaaaatca taacttcttt
     2341 gccttttctg gattcgtaag cgttgtagtt cagggaaaaa ttaataattc ttctatcggg
     2401 gtaaagagat tttatttctt ttttattatc atatgtgagc agccaaaaag catctggtga
     2461 tttattcaat ttctctgcta aggattggtg atcgtctttt ttgtaatggt ttaaataaag
     2521 cgtggagcct ttttcataat aaggaggatc aagatatata aaagcatttt ttttatttaa
     2581 gtattcagtg attaaatcaa gcccatcttt gttggtaacg gttattctat ttttatattg
     2641 agatagctga ctaatctttt caataagttt ttccttatta aatctggcgt ttattttata
     2701 ttttcctaac tgcttaactc caccaatagg accaccatct aaaattcctg aaacattagt
     2761 cctgtttaaa taaaaagtgg caaatcctct gtctagctgt tttgcctttg catcgtggta
     2821 aatagttttt tgtttcctcc attctttaat gtttatcggt gttgaattta tttttttaat
     2881 aaatttttct gaattaaaaa tggcagattt ccaaaaagaa tatatagctt tatcaagatc
     2941 gtttataacg atatgatcaa ctttttctaa aaataataaa gcaagggcgg caccagcccc
     3001 tcctgcaaaa ggttcaacat aagttatgcc gtcaagttta ttgtctttta ttactttatc
     3061 aaaaaagggg aaaagaaaag tctttccacc aggatacctc agaggactgt aatgaaattt
     3121 attatttttt ttatggctta ttttgaacat ttggtttttt gttaggagct aatatgtagt
     3181 taaataatgg ctccatagca tcccatgcgt cttcgacctc ttgcggggtg acaaaaatat
     3241 ctgggttgtg attcgttgca tttaagaaag tggcggaata ggaattcatt tttgaattat
     3301 ctttaatcct tgatgccact tgggaaagtt tattattttt tgactgattc atttctttaa
     3361 taaactcctc aagaactgtg tcaagaaaaa catatttccc cccattcttg ggggcaatag
     3421 tttttttaca gtgatcaaaa tatgctttca atccgcattc taaaaaactt cgaagcagat
     3481 cgtgtgcggc gttcggaaat ttatggtaat caattgcttg caactccttc agcattctct
     3541 tgactccacc gcattgcaaa gtaaatgata tgcttgaagg tgccagcctg tttctttttt
     3601 tggttgttac gatcaatgct tccgtaaaat ctttgctggt cgttactttg ccggttttgc
     3661 ttttgtcggg cgtattttct ttaacaaaat ttgacaggta ttcctttctg tctttttcgc
     3721 tgtttaatgc acgagaatca acattcttct caacaacatc ctcaacgatc tttttgaaac
     3781 ccttctcgaa ttctttctta tcaatattga tttttacttc gccgtttccg tcaaaatcaa
     3841 aaccgagaaa atccctgact tgtttatcat cgtataatct ttcgatggta gatgccggga
     3901 aagttttttc attgtgaact ttcttcgctg tagaatcaga atcgaatgtg atagattttg
     3961 ctattttgtg aatattaatt aatcgcaaaa acttactgat atcaacggtt ggataatcgt
     4021 ctctcagatt ctgaaccgtt ttgcctcgct caagctccgc tttataaaaa taggcttgtc
     4081 ttaacggtct ccatcttctt tttgtgtttt gagtatgttt attggccaaa aaaaccatcg
     4141 ccgaatcacg atcgggagca attacaacct cacgctcatt tgccacagga atagcggtct
     4201 ttaaaatgcc ttttatagct gactctttcc cgggtactat ttccggtctt aataatgctt
     4261 tgacggccgc aactcttcta tttccctcaa ggacgatgta tttgctgttt tcttttacaa
     4321 caacaggaat ctcatccgga aaaaatccgt tgatggcaat gctcctcaaa acctgcatcg
     4381 catcctcatt ggtaaataaa tcgtttatca gggcatcctg cgacaagtcg acgccaatat
     4441 caagacgaat attttttgtg tcgagaaaaa ggtcagtaac ctttattttc tgttttggcc
     4501 attcttcgta atttggtttg gcgatttctt ttttcataaa attcttatta tattatatca
     4561 atttttttga ttactggcga atttattatt cgttacccca atccttgcac aagatttcgg
     4621 cttgctccaa aacagtcttt gtagcttgtt cttgcttatc cggtgggtaa ccgtatttat
     4681 ttagtaattt tttaacatag acacgaagct tggctcgaac ctgctcttta agcgtccaat
     4741 caatcgtggt atttttgcga agcatagcga ccaactcttg agctatttgt ttgagagtgt
     4801 cgtctccaat ttccatgaca gcactgtcgc tctcacaaag ggcatcgtaa aaagctatct
     4861 cgtcttccgt gagatttaat tcattgcctc gttctttttc ctcgcgaatc ttttttgcca
     4921 actcaaccaa ttcggcaatc acttgagccg cctcaatggt ttgattttga tattttttaa
     4981 tagtctcctc gagcattttc acgaatgaac gagatttgac caagtacttc ctttgcatcg
     5041 ttctgatctc gtcgttcaat aattttttca acaattcgag agcgatgttt ttgtgttcaa
     5101 ggtttctgac ctcttctaaa aactcatcag acaataccgc aatattagga gatttaagtc
     5161 cggccgcatc aaagacatta accacttcgt cagaaacgac ggcattggat acgatctgtt
     5221 taatagcctg atcgtagtct tcttctgtcg gtccgccttg gccaccgttc tggtcaaatt
     5281 tgcgaataac agcaccgaca gcctgaaaga atgccacatc atcccggatc tgtattgatt
     5341 catcgcttgg aacagccaac gagaaagcgt gagacaattc ggtaacagct ttaagataac
     5401 gatccttgcc tttatcaaga ctcaagacat aatccatggc accggaaata acccttgtct
     5461 tttctgaagc tttggatgag aaataagatg agtaatcaaa ctgatgatat agagctttta
     5521 caacctcata tttttccaac atgactgaaa cagcttcttc ctgcggaata gtcggggctt
     5581 ccttgtcgtt gtcagcgtaa taagaaaggg cttcttttaa agagggagcg ataccaagat
     5641 agtccacgat cagaccgcct tgtttgtctt tatagatacg gttcacacga gcaatggcct
     5701 gcataagccc atgaccctgc atcggcttgt cgatatacat cgtatgcagg acgggcacat
     5761 caaagccggt caaccacata tcacgaacga tcacgaattt gagatcgttc ttcgggtctt
     5821 tcattctttt ggcaataagc tctcgttcat ccttagtatg aatgtgcttt tgaaaattag
     5881 ccggatcact ggcagagccg gtcattacaa cttttataaa acccttattg atatcatcgc
     5941 tgtgccattg cgggcgaagt ttgataattt catcatacag ctcaaccgca attcttcggc
     6001 tcatggatac aaacatacct ttgccctcca ttgcgtcacg gcgagcctca aagtgatcaa
     6061 ctacatcttt ggcgatcagc ttgatgcgtt tatcagaacc gaccatagct tccagtcgag
     6121 cccatttgct ttttagtttt tcctttttcg caacttcttc gccctcagtc aattcttcaa
     6181 actcagtatc aatgtgtggt ttttctgatt cagacaactg gatttttgcc aagcgtgctt
     6241 cgtaataaat tggaacagtg gcgtgatcct caaccgcacg agagatatca taaacatcga
     6301 tatattctcc aaagaccgct ctggtatttt tatcagataa ttcaatggga gtaccagtaa
     6361 agccaataaa ggatgcactt ggcagagcat catgcatatg acgagcgaag ccatcaataa
     6421 agtcatactg actgcgatgg gcttcatcgg caatcactac aatgttgcgt ctgtcggaca
     6481 atagcggatg tttttcgtct ttattctctg ggaggaattt ctgaattgtg gtaaagataa
     6541 caccgccgga agcaacgctt aataattttt taagatcggc acgatccttg gcctgtgtgg
     6601 gagtttggcg tagtaattcg tgacagttag agaatacgcc gaataactga tcatcaagat
     6661 cgttgcggtc agtaagaatg acgagtgttg gattatctaa aacctgaatg attttgccag
     6721 cataaaatac cattgtcaga gatttgccgg aaccttgagt gtgccaaaca acaccagccc
     6781 tgcggtcgcc tttttctgaa gtagcaccga ttgttctgat gattgcttta ttagtagcat
     6841 gatattggtg ataagctgcg agttttttga ttgtagtttc tccgtcagtt tcaaagacga
     6901 taaaattctt tgcgatatcc aacagatgat cttttgtaaa tacacctttg atcaaaactt
     6961 ccatttccgg aatagaagct ggggcgacag tctcgccatc tattgttcgc catttcataa
     7021 agcgttccat atcagccgta agcgatccaa tgcgactatc aattccatcg ctgattatgc
     7081 agaaagcgtt gtaagtaaaa agagaaggaa tgtctgattt atatgtttgg atttgattga
     7141 aagcgtttct aacggtagcg ttaacatcag ccggattttt caactcaaat acagccaatg
     7201 gcaagccatt gatgaaaatg atgacatcag gacggcggtt gtgattattt tcaataacag
     7261 tatattgatt gaccgccaac caatcgttat tagataaatt cttatgatct acaagataca
     7321 ctttatctcc agcgatcgaa ccatcctcac ggcggtattc aacatcaaca ccctcagcca
     7381 gcattctgtg aaatgagtga tttgtttcca atagacttgg attggaataa gcaacattaa
     7441 caactttctt gatagcgtcc tcttgggcgt ctcgtggaat attgggatta attgctgaaa
     7501 cagctgatcg caatctgccg atcaagacaa tatctttgta agactcacgc tccggtgatt
     7561 cgccatcggg agcaatgttc ggtccaaaga caattccaaa acccaactcc tcaagccatt
     7621 cgagcgttgc gttttccaag tctgattctg tgtatttgta gttagtcata atatttacga
     7681 tttattattt ttagcttgta tccatccgaa caaatgtctg aatatttgtt ccgaaatact
     7741 gaccattgcc tccgttgctt ctatgattga tttttctggt atgttctgtt tatcggaaat
     7801 cacaaaactc atttgaccgt cctttttcaa ttcctccacc gtgctgaata tgtgcttgtc
     7861 actgacatgg ataaattttg atgattcgcg atatagattg ataacccact ctgtttcttt
     7921 ttcctttgat aatgattccg ctaagtaggt atctgtcata aattgcccag tcttatcttt
     7981 taatttgctc gtccgttctc cttgcagttt actcattgaa aattcgtgcg gatcaggagc
     8041 aataaacgag gcatgcaaat ttagtaaatt atccaaatgc aaccgaacaa taggtccggc
     8101 acttaaaaaa ttattttgcc ttatcaaact acaaaaaccc gaggttaata aaattgatcg
     8161 cttaacaata ccgagcacaa acaaatctag cggataaaga ttgccattgg cagactccag
     8221 catttcttta gtcaccttga taaaaggctc ttttgaagtc tctaatttat aaagtatttt
     8281 gtctaaattt gtttgatgtt ccataacatt atttcactac tatctttcca ttgagtaatc
     8341 tgggaagtaa agaatcccta agtattaata aatttcttat ttcgttatga ttttcaatca
     8401 acattttaag tataggtgat gcaatcttgg aaaaacgatc caacgcttct tgggatggta
     8461 caactatttc taaagattta gtttcaatgg ggctcagatt ttgttgagct gtgccacgag
     8521 aaatactcat caatgtattt tgaaagtcct tttgtctaaa aaggaaatat gtaaaagctc
     8581 ggttattttc aataatcgga atcagatttg ccacacgctg atttaataga taattagacc
     8641 cgttcactaa acatactctc cccacattac cagtcagcga taacaaaata tctccgtctt
     8701 ttagtttgca atgggttggc attttctgtg ggatttcact cagtgagtct gtgcattcag
     8761 ttataaaata tccgtcttga acatttttta ttgtaaccaa cttgtattta ccaaaatcat
     8821 caaacatcct actgttaaat gggaatccgg attcgacttt gacaacatca gaaacctttc
     8881 caattttcca gcctgatggc aaattatctt taacatcaat aaaccattgt ttaaatatta
     8941 tcccaaccaa tgtttcgaga ttattgttta tctttctatt tagttcaatt ttgtcatcga
     9001 tattccacaa tatggaagat atcttttttt gttcctcgat tttaggtgtt gctataaata
     9061 gatttcttac taaatcagtg tttaaatttt gcaacacagc tccatttgcg taggtgtcgg
     9121 agactctttg cccattgtaa agccaccagt aataaaaaaa tttatcatct aaatttttat
     9181 actccgtaaa ataaagccaa ccgtcatgaa tacatccgtc tactgcaaga aattttggta
     9241 ttcctgccga tccgctattg gatacgatta aagagccttt ttttaaaaaa acacttctat
     9301 ttttaccttc ttcagttacg gtttctctgg tctcttcaat gtatctcgta ccggttctat
     9361 ttgcatctgc aattttgatc caaggtgttg atccaccaaa atattttgga tctccaactg
     9421 gtcgaggtga agcacctctg ccgactttac ataaatcgtt tacagcaaag acaccccatt
     9481 cattggggac tgttcctatt tttgtatctt tgtattttgt ggcgatttcc atatataact
     9541 attttatttc aaaaccgatt tcttttaagt cgtgttttat ttgattttca atttcttttg
     9601 atttcttgaa ttggtcagaa agatttgcgg ttactcttgt catcttgtct tcaaacgagc
     9661 tgtcatcttc ctctacaaaa tctgaaccaa cataacggcc gggagtaaga acaaacccgt
     9721 ttttttccac ttcctcgatt ttagctgctt tgcagaatcc cttcacatct tcgtatttgc
     9781 cattaatggt tcgccaagcg tgatatacgc cgataatttt atttaaatct tcatcagtca
     9841 attcacgatt cttgcgagtg accattgttc ccattttgcg agcgtcaatg aaaagaatct
     9901 catcatggcg gttgcggaat ttatgatcgt agcgatcacg agacacgaac cacaaacaag
     9961 ctggaatttg tgtggtgtag aatagctggc ttgggagagc gacaatacaa tcaactaaac
    10021 cattttggat tagacttttt ctgatttcgc cctcaccgga agtattggat gagagcgatc
    10081 cgtttgctaa aacaaaacca gcaatgccgg tgggagcaag atgatggatc atgtgctgaa
    10141 tccatgcata gtttgcgttg ccggctgatg gtgtgccgta tttccagcgg acatcgtctt
    10201 ttaagcgttc accaccccaa tcactgatat tgaaaggcgg atttgctaaa ataaaatctg
    10261 cttttaaatc tttgtgtaaa tcattatgaa aagtatcggc attatgttgt ccgagattgc
    10321 cctcaatacc acgaatggca aggttcattt tgcaaagtct ccatgtggta ggatttgatt
    10381 cctgaccata tatagccaag tcatgagttt gaccgccgtg tgcttccaca aatttctcac
    10441 tctgcacaaa cataccgcct gaaccacaac aaggatcgta gacacgacct ttgaacggct
    10501 caatcatttc aacaagaagt ttgacgacag actggggagt gaaaaactct ccaccgcctt
    10561 tgccttctgc tccagcaaat cgtccgagaa agtattcata gacacggcca agcacatctt
    10621 tgcttcggct ggctttatca ccaagaccga tagagctcat aagatcaata agttcaccaa
    10681 gtctaacttt atcgagatcg gggcgagcgt aattttttgc caaaacaccc ttgagactcg
    10741 gattgtcttt ttcaatcgca atcatggcat catcaagcat ctttccaatt tccggttttt
    10801 tagcattagc tttcaaatat tcccagcgag cttcctgtgg cacccaaaac acaccttcgg
    10861 caagataagc atctttgtct tcctcctgac cagcatagta atccttgtca cttttgattt
    10921 cgttatagac ctcctcaaaa gagtcggaaa tatatttgag gaaaatcaaa ccaagaacca
    10981 catgcttata ttcagccgga tccatgttat tcctcatttt gtcagccgcc gaccagaggg
    11041 tttcttcaaa gccaatgtta gcggaagatg ccttttttgc ttgttttgcc atatattatg
    11101 attgcttatt tatgcgatat ttttatagtt ccattgtacc gcacttgact ataatttgtc
    11161 aataatatac aatggaaata agaaaagtta tcaattccat caaataacat agatatggac
    11221 agcttaatcg caaaattata tcaatcacca aaaacaatcc tgtcgaataa ggatttggcc
    11281 ttaatttggc aagaaaacga taaattcaac ctgaatgcca agatagccta ttatgtaaag
    11341 caaaaagcct tgatcagact gacaagaggc gtttttgcca agagtaaaga ctacaattca
    11401 aaagaattgg caaccagtat ctacaccccc tcttatatca gctttgagac agttttgaga
    11461 gaggctggcg ttatattcca gcattacgaa acgatcttcg tggcatcaaa gtggccgaaa
    11521 acaaccaaga tagacaaata cacgatcaca ttcaggaagt taaaagatgc tgtgctgttt
    11581 aattcaaccg ggattgtcag taaagacaac tacagcgtag ccacaaccga aagagccttc
    11641 ttggatatga tttatttatt cccgaattat tactttgata atctgaaatc aatcaactgg
    11701 gaaaaatgta acgagatcgc aaaaatttat aacaacaagc aattgataaa acgattgaat
    11761 aaatatcgca aaaattatgc tcaatagaga aaaacatcaa ttaataatgg gtcagatttt
    11821 gaaagacatc tacaccaatg tctcaatcag tccgctctta ggattcaaag gcggtacttg
    11881 tgcctatttc ttttataacc tgccgagatt ctcggtggac ttggattttg atttgctggt
    11941 agtgaatgag gaaaatcaaa agttagtatt tgaaaagatt gtcgctattt tgagcaaata
    12001 cggacagatc aaagatcaat acataaaaag atttactgtt tttgcattac tctcctacgg
    12061 cgatgacgat cacaacatca aggtggaaat caatgtcaga aaattagtag aaaatatcca
    12121 agaccactac gagatgaaag agtatcttgg catttcaatg tttgtcgcca aaaaagatta
    12181 tttgtttgcc agtaagctct cggctttaac actccgaaat gaaacagcaa tgagagacat
    12241 ctatgatatt cattattttg ccaagagtaa ttgggacatc aatactgaag tggtcaaaga
    12301 aagaaccagt aaaacagtaa aagaatattt ggctgattgc atcgctttta ttgagaaagt
    12361 aaaggatagc cagataatgc agggattggg cgaactgatt gatagtgaga aagaaaaagc
    12421 atgggttaga aatcatttga aagccgacac tatcttcatg ctgaaaaatt acatgtcggt
    12481 gataaaataa aaatatatgg caaaaaaaga acagaaaaaa catactcccg aaccggaaaa
    12541 atcagataag agatatctga aaaagtttga gatcgttgaa accgatgtcg aacttcctat
    12601 ccaagggcaa gtccgcgatc cggaggattt atataaattc ttaaaagatt tggaaaacga
    12661 aagcgtttcc aaggttattg gaatttattt ggatgacaag aatctatttc tcggacatca
    12721 agtattcctc ggatcttcgc ctaaagcttt tgatacgcaa ttgctctacc attactacgc
    12781 cttattttta gcaaagaaat tcatcgtctt aattaaccat cccggatcaa ccgacccgac
    12841 tcccgatgaa gcagataaga aattaatcag ggctttgcaa gccgatgcac aagtcttgtc
    12901 attcaaacct ttttttgccg atttcatcat tgtggccaag cgatcatact tcagtatgtc
    12961 gacaaatgac ggcactgcct gccattgcgg acatcaggaa tacataccgg aataacattt
    13021 aatataaaaa ctatggaaaa taatatgaac aaaatagcac ttggagtatc atttggcttt
    13081 actggtggtg caatatttgg aattattctc ggcctattaa ctaaagattt cgcagtttgg
    13141 ttggctgttg gtgtgggatt cggaatgatt attggatcaa gtgtgggatt atttcttaaa
    13201 aacaccaaaa gaaattaata ttggttattg ttataaaata ttatgcacaa aaaaactatt
    13261 gtgctgtttt ttagaaagta gtataataag tttaacaatc aaatagagtt atgccttacg
    13321 aggcatcctg aaaggaattc cgggttcaag agctcgtgat ccattatcac ttttgtggta
    13381 atcggtcacg agctcttttt gtttgattgt tgccagctct ctgccacgcc caataacggt
    13441 cgacatccaa tcttgttaat cgacctcaca ctgcccgagc tcaagggttc gggtaataaa
    13501 aaatatacaa gggatgtggg cgtgccagag atttgagaat taaaaaaata tgtttagtaa
    13561 atttcaagcg ttgccgagtt acttcggcgg aaaaagaaaa ctggtcaagc acattttcaa
    13621 accgatcaag aaaccggacg gaatttttat tgatgcattt ttgggaggcg gatcagttag
    13681 tctctacgcc aaggccaaag gatataaagt cataagtaat gacattgcgt tccgatcagt
    13741 gattatcggc cgagcgatca ttgccaacaa ttcagtaaag cttgaatccg aggatttggc
    13801 cagactattt tttgaaacca aacatgacag ctttataaaa aagaattatt gcccgaaagt
    13861 attcaccacc aaaatcgccg actttttaga taacgccttc gcttccgcca acaaggtgga
    13921 aaatgatact aaacgatacc ttttgttgca tttattgatt aagtttattc tatcctgccg
    13981 acaattcggc aaatttacac atacccggga cacgctggac ttggaagccc gaaaatggga
    14041 gtggcctctt cggtccaaat cacacgcccc gaaaaatttg cggatgatac aaaatccgat
    14101 gatgacctta gaaaaactga aagatgagat aaaccacggc atctttgata atcaccaaga
    14161 gaacgaaata caccaaaaag atgtttttga ttttttgaaa gatgttagag gcgatgtcgt
    14221 gtactttgac cctccatatc ccggatcttc tgcctatgag gtggaatacg gaatcttgga
    14281 caacatactt gccaacaaaa agctggataa caagccgagc gatttctcca acaaagatgc
    14341 cgatgaattt ttagaaaaga tgtttgagtc cgccaagcat ataccgcagt ggataatctc
    14401 gcttggccaa aacaaagagg atgtgggagt gaagccggaa aagcttttgg cgatggtgca
    14461 aaaacaccgt aaagccgaag tgcagatgct tgatcacaaa tggagtgtgg ctaacgcagg
    14521 aggtcgaaac caaagcaaca acatcgaata catgatcgta actttatgaa aaaagaaaaa
    14581 accgaaatca aaagagtgaa aataaaagag tgcgttccaa actggtggaa tccgaacgaa
    14641 atgaacgctc gccaattcac atcgctgaaa aaagctttga ctaagtacgg acaaaccaaa
    14701 ccgattcaag tgggagtctg gccgaatatg aaagccaagg ccggttatgt gattgtgggc
    14761 ggttatcaca cttggaaagt cctcggcgaa ttgggcgaaa ccgaaattga agccaatatt
    14821 cataatttca aaaatgagga cgaagccaag ctttatggat tgaccgacaa cattcacgga
    14881 tcgaaccaac agataaaact cggcaaactg gcctacgagc tgactcaaaa cggatactca
    14941 atcaaagaca ttgcccagag tctcggcgag gaggatatca atgtcaaaga tgctctcgac
    15001 ttggtgaaag acgagattga aaagaaaatg atcgagctta agaaagccga gcgagaaaac
    15061 accgttgtcc tgaacttcat cgtggataaa gacccggaag cgtcagccga tgaatttata
    15121 aaagccgtga ctgcatttgc caaaagcaaa ggcgtaaagg tcgagaaagc ggtcaaagat
    15181 ataaacagca aaagagaaac cattgccctt gtaagtttca atgtcaccaa tccgcaaaag
    15241 ggcgtgatag acgaagcgtt aaatatcctg tcaaagcaat caaaaatgac agagagtgag
    15301 gcgatcgccg aagtgtgtag ccagtttata aacacactaa acggcagtaa accgcagtaa
    15361 acagcaacga aatgagcaaa aaagtcgaca ttaaaagcat caggtatcaa gaagcgattc
    15421 gccttaaata caacaacgaa tcgtattctt cgattgccaa aaaaataaaa gtgccggtgt
    15481 cgactatcga aaaatggttc gcttttggcg gtttgcttaa agaggagtac gaaaaatacc
    15541 gtgatgaaca gaatcaaatc agacatgaaa acggtctgga agttttcaag aaaaatgttg
    15601 aaaccgcctc aagcatgatc gttgccttaa tggccagcga aaatgacacg gtgaagctta
    15661 atgcctccaa agagatcgtc taccgccagt acggaaagcc gaaagagaca gtagaacaca
    15721 aaggattatt tgaatcaggc ctgaattatg aacagatact acgaaaatca agagacaaac
    15781 cacccgatgg ttaataacct cattgctttc ctgaggttat gcgaggctga cccgaaagtg
    15841 tttattgagg agtgtctgtc cataaaaacc aaagaccaaa aaaccgttct tttaaagttt
    15901 aataccgctc aatccatgat ttacgaacga atcaagatgc taagagcaaa aaagaagccg
    15961 atcagaatga taattctaaa agccagacaa gagggcgttt caacattctg cgagggattg
    16021 atatttgaaa agaccgttcg tcaagaaaat accaattcgc tgattgtcgc tcacgagccg
    16081 gagtcgactg atgcgatatt cgccatgagc aagctgttct acgacatgct tccggtggat
    16141 gttaagccaa tgaggcgata cgacaacaag aaacagatgg tgtttgaaaa tcccgatcag
    16201 aaagcgagag ccgatgaccc gggactcaga tcaagaatgg tgatatcaac ggccgataaa
    16261 gtaaaaattg gtagaggtct gactcttcat aatttccacg gatctgaagt cgcattctgg
    16321 aaaaacgcca aagagctgat gctttccgtg atgcaagcga ttccaagcct gccaaccact
    16381 gccgtctttc ttgaatcaac cgccaatggt tttggcggtg acggcgaata cttctaccaa
    16441 atggtgcaag atgctttggc cggtaagaat gaattcgagc ttatattcct cccgtggcac
    16501 ctgatgccgg aatacagtca accttttgcc agtgaagccg agaggaaaca gtttgccgaa
    16561 accttgaacg aatatgagaa agaattgctg aacaaagaaa acctgagata cgagcaattg
    16621 aaatggcgta gatgggcaat tcaaa
//
LOCUS       DAOOYB010000036        16628 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_3398_length_16628_cov_7.292222, whole genome shotgun sequence.
ACCESSION   DAOOYB010000036 DAOOYB010000000
VERSION     DAOOYB010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 16628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16628
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3398_length_16628_cov_7.292222"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /locus_tag="PL056_03545"
     CDS             <1..737
                     /locus_tag="PL056_03545"
                     /inference="COORDINATES: protein motif:HMM:NF016439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase sigma-54 factor"
                     /protein_id="HPM46126.1"
                     /translation="LSLDKEDLTELLQFFRKGVSPYPSFGYEKINTEFVRPDIKVFMV
                     NGEPVIQIEDKMLPSVVLKTEVFLEKLNTAKTREDRRFMKEKYRNAEWIIKSISERNR
                     TLYQVAASIFNYQKSFLELGENFLKPLTLKDVSDDIDRHPATVSRLTNGKYAETPHGI
                     YELKYFFVKQVNDNLTTNKRLEQMITEIVEKEDRNNPLSDDDITNLLSREGVEVARRT
                     VAKYRSKLNIPLARERKRNYQFSKGN"
     gene            740..1192
                     /locus_tag="PL056_03550"
     CDS             740..1192
                     /locus_tag="PL056_03550"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HPM46127.1"
                     /translation="MCRIEAYIKKELVHCKVPIKTKDGLFRYLSDIVEEVAGVSSETV
                     LESIQERESFGSTGIGHGVAIPHGRIPGCREVVIHVLTLSGKIPYGSIDGEGVSLVFM
                     LAVPEGNNLLYLKLLSRISIICNDSSIRDMLINAKSQEEMIRIIKEVP"
     gene            1192..2148
                     /gene="hprK"
                     /locus_tag="PL056_03555"
     CDS             1192..2148
                     /gene="hprK"
                     /locus_tag="PL056_03555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HPM46128.1"
                     /translation="MPTSGSLNLFELLNSPELELSLENMNEEKLRKIEVHDNRVQVVG
                     LSIAGFVDHLYKDRIQLFGKSEMDYLDHLKPKDRLKMSDTFLKERPILVIFTHPIKPP
                     DYFSHAALKYKVPLMSTSMTSSVFVDYIRGFLAAKLSVYTTLHAQLIDVLGVGMLIMG
                     ESGVGKSETSLDLVMRGHKLIADDVVEIKHIPPDRLLGGCNEMMKSLIEIRGIGIVDL
                     QQMFGITSVAAEKEIDCIVRLALWDEKHHFEYERVGSTANYYEILGVKKPYYVIPVRN
                     GSNLSSIMEATARDYLLKKMGINAAEQFENKLSEKLKKIGGN"
     gene            2148..2576
                     /locus_tag="PL056_03560"
     CDS             2148..2576
                     /locus_tag="PL056_03560"
                     /inference="COORDINATES: protein motif:HMM:NF015567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46129.1"
                     /translation="MYSFIFITHGDLGRTMLDIASRIMDEDVSEGCRLFTIDFAMVTE
                     LEEIKNNIKAAAEKFIAEGRKVIIFVDLFGGSPSNIAFTIAKNENVDVLSGVNLSMVM
                     YAIEHRNSGKTLPVMVEGIMKSGLQNITSAKKLLESREKK"
     gene            2573..2842
                     /locus_tag="PL056_03565"
     CDS             2573..2842
                     /locus_tag="PL056_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005882018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HPM46130.1"
                     /translation="MKDIELKITNKLGMHARAASMFVKTAEKYKCRVEMEKDGVRVNA
                     KSIMGILMLAAPVGSVIRLITEGEKEEECLKELADLIVNKFGEGE"
     gene            2844..4601
                     /gene="ptsP"
                     /locus_tag="PL056_03570"
     CDS             2844..4601
                     /gene="ptsP"
                     /locus_tag="PL056_03570"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HPM46131.1"
                     /translation="MRERVLLKGEKISSGIGIGRLFFIDRKFVSIPHISLAGGREAVL
                     NEIERFKKAVSSSEHELAKIISDKKTPGQTRSILEVHRMMLTDPTVSELVLKTVTEKK
                     INAEWAVLDVFSGLVEMLDRSSSGYYIKAKIVDLEVIRDKLISQLTGGVSPTVLEMPE
                     EDLVICSHSLTVSELNYFSQFPNVKGIVLESPGGVSHLTVVLRALEIPAVMGVPSLIS
                     SIDYGDIVIVDGVNGEVVLRPDRSEIKDAIRKNKRFEKYFARFLENVETPSVSKDGRI
                     LKIGGNIDQPGEVPFVKKYGGEFIGLFRTELMFPEKQVVPSEERHYEIYSQVLHFANP
                     LRATIRIFDFGEDKEGAIVHKGFMGMRGIRLCKRRPDVFIPQIRGLIRAAEQGNLNIL
                     LPFVSNVHEVDDFKELLFKEAAKMGLSRNLDNVELGAMIEVPSAIFIAEQIAKEVSFF
                     SVGTNDLIQYLMAVERKDQYSSDYFSHFHPSVIRVLFNLAHIAKEQGKEISICGEMAA
                     DRYFPLVFLAMGISSLSMSPISIPIVKKIIKCGYVKEGDMMLKKMLVAKKNSQIIKIM
                     RDFMVDRYPHIFTETWNNN"
     gene            4615..5514
                     /locus_tag="PL056_03575"
     CDS             4615..5514
                     /locus_tag="PL056_03575"
                     /EC_number="2.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate butyryltransferase"
                     /protein_id="HPM46132.1"
                     /translation="MTFKSISSFIEMAKAGEKRKIAVAAAEDEPVLMAVKNAIAEGIV
                     EPILVGDEKQIREISAKIDFSLDGIEIINELKPANACRKAVALIKEKKAGILMKGLVG
                     TADLLKAVLDKENGLRKGSVISHIAFFESPYYHKVFIVTDAAMNVAPEFKEKVAIVEN
                     AVDACHRLGLEKPKVAVIGAVEVINEKMEATVHAGMLTMMNKRGQIKGCIIDGPFALD
                     NAVSKEACHHKKIETEVGGDADILMCPDIEAANVLYKSLNFLGGAESAAVIMGASVPI
                     VLTSRADSEKSKLNSIALAAAMK"
     assembly_gap    5656..5755
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5756..6358
                     /gene="buk"
                     /locus_tag="PL056_03580"
     CDS             <5756..6358
                     /gene="buk"
                     /locus_tag="PL056_03580"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HPM46133.1"
                     /translation="LNQKAVGRKYAREKNKKYEELNLIIAHLGGGISVGAHKKGKVVD
                     VNNALNGEGPFSPERSGGIPSGQLTKLCFSGKFTSAEIHKKIKGEGGLVAYLGTNNAY
                     EVEKKVAAGDKHAELIYRAMAYQISKEIGLLATVLKGEVDAIILTGGVAYDKGFVAWI
                     KEHVSFIAEVVVYPGEDEMTALAEGCYYGIKGEITVRDYE"
     gene            6351..7919
                     /locus_tag="PL056_03585"
     CDS             6351..7919
                     /locus_tag="PL056_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46134.1"
                     /translation="MNRSCLLFFFILAVISGCSDTVIKKDLLKPGFYELCYSFPKLPE
                     NTNENDIRTASEAYEEHLTGNGITVLKRRYAADSVCYFTDSIKDLSLPRVELENNISQ
                     YLLTAYFNSEHSSLYGVFQGKRRSEKITDNIPFLRKLPIYIEKQEGLVISGTNRKMLF
                     VARYLDVLEIENEFGFNDKGRMVISVRSDESERWKMIPFLFDLLNLDEFVSLLPGDYA
                     VLNECREEKVSLKLSIPAWAVYSVPVAKDKGVVNEDALVFSVSSMSLPFDEETLLKYF
                     YSLSKSYLLPPSSGLYYSPNFKGSNVSPTVLLFSSELAISGEYIISPEMIYMWVSSVK
                     TQLEQFPDSFYANLLALKIARKLYTGTPSFLGGSELVNKTTAVPFENIAKMLFHTGNV
                     TVFGSISSSLYLYNGDIISEFSNLIEMQKVARVKALFGSPENSDRSIISFVVRSANAE
                     VLTGRIGDDLKKEGFDPTHLLFEQVSLNDAWGSVSVSVDKKSEKKILDLYEKKFKLLN
                     NTMSIGVYRVAKEE"
     gene            7900..10203
                     /locus_tag="PL056_03590"
     CDS             7900..10203
                     /locus_tag="PL056_03590"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HPM46135.1"
                     /translation="MSQKKNDAVKKKKKGSGEGSSEKKLIIIEISAAFVFLSAIFLWF
                     SLANFSAKSVRSGFFTGTLGYDAAHSLINLFGIVSFYIPLLLALTILCFIKRGFIRHV
                     ILNYLNFFVLLPLFWMISGYFRFYGIKNENFLGKLIYENIFHLYLGKTGFILLTTCWM
                     IIFSIFSFNVSYFFIFRKMVWFWSRFFEKVKETKLIDRDDPGQDDIDENDGVEEELTE
                     NSDDRKEFSFEPPPVPEKIEEKIEKVVEEKKITKRKDDISVSIAEQDGTGLERKTVRS
                     HPDYSGYKFPPLDLLKGEEKVKAENDREKKVRERALLLERKLLEHRLKVAVQGATVGP
                     VVTMYEVELGEGVRVNQVMAMETDLGVAVGGRKIRVVSSLPGKPYIGIEVPNDDRLMI
                     RLKTILRSDMFVKQSKTGLPIALGQDVSGKPFAANLVKMPHLLVAGTTGSGKSVGINT
                     FIVSLLFSLKPDEVKFIMVDPKGNEFNIYEGIPHLLLPVVVDSKKAAKALQWAVNEME
                     ERFHRLAENMVRDIEAYNEKVDNINKNLANVEHHMKKMPYIVVVIDEFADLMMVAGKD
                     VEIAVSRIAQKARAVGIHLIIATQRPTRDVVTGLIKGNLPVRIAFRVASALDSRTILD
                     SNGAEMLLGNGDMLYIPPGSSEAERVHGAFVGTDEIKKIVKFIKDQVGDDVATDDSIL
                     GSSPIDFLEDEGEGGYGGENEKDPMYDEILAYVLSTGKCSASMLQRKFKIGYNRAARA
                     VEVLEAQGIVSPADGAKPRTVINPKEG"
     gene            complement(10204..13311)
                     /locus_tag="PL056_03595"
     CDS             complement(10204..13311)
                     /locus_tag="PL056_03595"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPM46136.1"
                     /translation="MNFPKFAVSRPVFVSIIFIIMGLFGLYSLKELPIDLMPDMEIPA
                     ISVITIYKGAGSEEVEEKVSKVIESALSSTAGMKNIYSRSMENVSTVTCEFDYGTDLS
                     EATNKVRDLLNMSRSYLPDDVEEPLIFKFDFSMMPIMFLSVTSKKENIRFKTEEIEDA
                     LTDPIKRIPGVGSVITFNRMEKKIIISAEKQRLSAYNLTISDLVSTVQAENLSLPAGN
                     IEIGSLDYTIRVPGEYKNIDEINETIVASTPSGLIKIKDVARVFWGAEDTRQFGLKDG
                     EYMVFMMVQKQSGANTVEIADAVTAKLEEIKPRLPDGFKIDILLDTSDFIKKTISSLA
                     SSVLTACFFVILVVIFFLRRVKSSLIVLLSIPASMIIAFAFLYGFGFTLNMVSLMSLS
                     LAIGMVVDNSIVALDNIMRHLDDGKNKIDAAIDGTSEVGGAILASTLTTIGIFAPLFF
                     VGGLIGILFGQLAGVIILTLTASLLAAMLLTPMVSAKILTRETNTNYDNWFFRAGEKF
                     LTYTEKLYTKVIVSTLKHRKKTVLAAGIIFIVSLVFIPIIGIDFMPEGDEGMIQIAFE
                     MPLGTKIETTLATAQHIERIMKEEIPQKYLLKTFMRGGPDYSGFSQKVDDTNTATVGA
                     RLVSQDYRKENVKVFVNKIRARIVKEVPGIEKMDMQTSSGMSNLMSGGEKPVTVKLAH
                     KDFTKINEAALRLQKELEKIPGLKDISNDSDSLKPQIEVKIDRIRASQVGLKTSMIGT
                     AVRNAFYGNTASVYRKDGDEFEIMVKYKKADRTNIEQLKELEIKTLMGTTVKLKDVAE
                     VKEGLTSLSVNRQNRERIVTIGARLEDNIAIGKIAVEVEKAIKNSNIPPDVEIIYGGN
                     IQSQKETTGDLVLMLLLGIVLVYLVMAAQFESFVDPFIIIFSIPFAISGVLMGLLLTG
                     HALSVPAFLGMIILVGIVVNNAIVLIDYTNTMKVKHNFTMDEALIYSGERRLRPILMT
                     ATTTICGMVPLAMMSGEGHETYNPMGVAVVFGLTFSTLVTLVLIPCMYSAVDSFLRKH
                     HLRKG"
     gene            complement(13308..14471)
                     /locus_tag="PL056_03600"
     CDS             complement(13308..14471)
                     /locus_tag="PL056_03600"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPM46137.1"
                     /translation="MKAKLVSAAAVIVLTLTTGLVSCSKNELTDSSNLTQAVKITKPE
                     KRKFSRSFDTASVAMAVKSAYINPKVAATIKSFNVKEGDFVNAGSTLAQLDGSDYEIA
                     VNASKAQLSAAEAGVMQAEAAFAKISADYERFKTLKQNGSVSDSEFEQVESGYKQAEA
                     GLAAAKAQKNMAQSALDGNTRQFNYTSIIAPFSGYIASRNGEIGEMASPANPRPMFEI
                     VQTDKLKIEIFISELEIGGIDKNTTAKVIFDAFPEKEVTARVNLINSKVDTMTKSIKV
                     EMLIDNPGNVFKSGMTVRVRFTLPEHEYLVVPRNAVFTRDNEAGIIYAKNEADRVFTK
                     EVFIGGSVEGYSIIEKGLDGSEDVVVGGGRRLEEGQKVNVISDNATAESEVKK"
     gene            complement(14468..15157)
                     /locus_tag="PL056_03605"
     CDS             complement(14468..15157)
                     /locus_tag="PL056_03605"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPM46138.1"
                     /translation="MARTSEEKKEMIIMNFVSLIQQHGINRVTLNDVAEKSGLTKSAL
                     YYYFESKEVLLIEGFEHFNKKIQEKLVPLIEKAKSPKDILFIYCDFQFKVFFGGYEEF
                     KPLMEISTEVFLEIQKYMFNSPDIAKKIMDHRDSELNWLNSVIAEYIGESPDDERTKK
                     ITLMFVGFMHSFVTMSCNITKQSKALASYSLISDFPWRIDNIDNTDIFKFLIGGLNNL
                     KRAIFNMENDK"
     gene            complement(15157..16485)
                     /locus_tag="PL056_03610"
     CDS             complement(15157..16485)
                     /locus_tag="PL056_03610"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPM46139.1"
                     /translation="MRKLLISFLFFVPFFIFSSELSLDECIENSVKESEKIKSAEAGA
                     EAAKAGKNAVIFSFLPTAKLEASYQWLKFDPEPEPVTMDLTAMGMGVVEFGAIPDKSR
                     TVGMTIVQPITPLWSVFNGYKAAEIGFEISKLQKELSIEQIKLEVMTLFYNYQMLSES
                     NKLLSETKEQLKRYYNQASNFVNAGLSDKRAVLKIDIEIARLDQQIQIVEGNLDLIKK
                     NLSIFMNMPVDSFTLKTVKPVEAALSSTASELENMMINNRIELKMIEKSSKIAKHMEW
                     MSAQPFIPTLAIAGGFKKTWDDSQFQPAMTMFIGGSLSWNVGFDWGKAGFEYYKAKKE
                     RLKTDLDNINTKKMLGLQLIQLENDVETKYQAIEVAKREIASATENLRIEEDKYREKM
                     TTETDLLDASISLRSAKLKLLTSMYEHLVALNRLAITVGVDYQTIVSGGE"
BASE COUNT         4775 a         3265 c         3772 g         4716 t
ORIGIN      
        1 aactttcgct tgacaaggaa gatcttactg agcttctcca gtttttcagg aaaggagttt
       61 ccccttatcc ttcattcgga tatgaaaaga tcaacactga atttgtgaga cccgatatta
      121 aagtgtttat ggtgaacggg gagccggtta tccagatcga agacaaaatg ctcccttctg
      181 ttgtcctgaa aacagaggtg ttccttgaaa agctcaatac tgcaaaaaca cgcgaagacc
      241 gtagatttat gaaggagaaa tatagaaacg ccgaatggat cataaaaagc ataagcgaaa
      301 ggaacagaac gctttatcag gttgcggcga gcattttcaa ttatcagaaa tcatttcttg
      361 aactgggaga gaatttcctt aaaccgctta cgcttaaaga tgtttccgat gacatcgacc
      421 ggcatcccgc cactgtttca agacttacca atggaaaata tgctgagact cctcatggaa
      481 tatacgaact gaaatatttt ttcgtcaaac aggtcaatga taaccttacg actaacaagc
      541 gtcttgaaca gatgataact gaaatagtcg aaaaggagga caggaacaat cctctcagcg
      601 atgatgacat aacaaatctt ctctcaaggg agggtgttga agttgcaaga cggactgttg
      661 caaaatacag atccaagctt aatataccgc ttgcgaggga gagaaagaga aactaccagt
      721 tttctaaggg gaattaaatt tgtgcagaat tgaagcttac ataaaaaaag aacttgtgca
      781 ttgcaaggtt ccaataaaaa ctaaagacgg gctctttcgg tatctgtctg atatcgttga
      841 agaagttgca ggcgtttctt ctgaaacggt tcttgaaagc attcaggaaa gggagtcttt
      901 cggttcaacg ggcataggtc acggagttgc catccctcac ggaagaatac ccggctgcag
      961 ggaagttgtt atccatgttc tcacgctcag cggaaaaatt ccatacggca gcatagatgg
     1021 cgagggggta agtctggttt tcatgcttgc tgtcccggaa ggcaacaatc tgctttatct
     1081 taagcttctg agcaggatct caataatatg taacgacagc agcataagag acatgctgat
     1141 caatgcaaaa agccaggaag aaatgatacg gatcatcaag gaggtgccct gatgcctaca
     1201 tccggttcgc tcaatctttt cgagctcctt aacagtccgg agcttgaact ttcacttgaa
     1261 aacatgaatg aagagaagtt gcgcaaaatt gaagtgcacg acaacagggt tcaggttgtg
     1321 gggctttcaa tagcggggtt tgtagatcac ctttacaaag acaggataca actgttcgga
     1381 aagagtgaaa tggattatct ggatcatctc aaaccgaaag accgtttaaa aatgtctgac
     1441 acttttctta aggaaagacc gatacttgta atattcactc atcctatcaa gccgcctgat
     1501 tatttcagtc atgccgcttt gaagtataaa gtccccctca tgtcaacatc tatgacatca
     1561 agtgtctttg ttgattacat caggggattt cttgcagcaa agctttctgt ctataccact
     1621 ttacatgccc agcttattga tgttctcggt gtcgggatgc tcataatggg agagagcgga
     1681 gtcggaaaaa gtgaaacgag tctcgatctt gtcatgagag ggcacaaact gatagcagat
     1741 gatgtcgttg aaataaagca tattccgcct gaccggcttc tgggcggctg caacgaaatg
     1801 atgaaaagtc ttatcgaaat aagaggcatc ggcattgtcg atcttcagca gatgttcggc
     1861 ataacctctg tcgccgctga aaaagagatc gactgcatag tgcggttggc actctgggat
     1921 gaaaaacacc actttgagta cgaaagggtt ggaagcactg caaattatta cgaaatactc
     1981 ggggtgaaaa agccgtacta tgttattccg gtaaggaacg ggtccaatct gagttcgata
     2041 atggaagcga ctgcaagaga ttatctgctc aagaaaatgg gtattaatgc tgcggagcag
     2101 tttgaaaata aactttccga aaaacttaag aaaatcggag gaaactgatg tattctttta
     2161 tttttatcac tcatggcgac ctgggcagga caatgcttga catcgcttca cggataatgg
     2221 acgaggatgt ttctgagggt tgccggcttt ttaccataga ctttgcgatg gtgactgaac
     2281 tggaagagat caagaacaac ataaaggccg ctgctgaaaa attcattgcg gaggggcgga
     2341 aagtcattat ttttgttgat cttttcggag gaagtccttc aaatatcgct ttcaccatcg
     2401 ccaaaaacga gaatgttgat gttctcagcg gagtaaatct ttcgatggtg atgtatgcaa
     2461 tagagcacag aaacagcggg aaaacgcttc ctgtcatggt agaggggata atgaagagcg
     2521 gtctgcagaa cattacaagt gcaaaaaaac ttcttgaatc aagggaaaag aaatgaaaga
     2581 tattgaactt aagataacga acaaactcgg aatgcatgcc agagcggcga gcatgtttgt
     2641 gaaaactgct gaaaaatata aatgcagagt cgagatggaa aaggacggtg tgagggtcaa
     2701 tgcaaaaagc attatgggaa ttcttatgct ggcggctcct gtcggaagtg ttatccggct
     2761 cattactgaa ggcgaaaaag aggaagagtg tctgaaggag ctggcagacc ttattgtgaa
     2821 taaattcggt gaaggggagt gatttgagag aacgggtgct tctcaaaggg gaaaaaatat
     2881 caagcggtat cggtataggc agactttttt tcattgacag aaagtttgtt tcaattcctc
     2941 acatttctct tgcaggcggc agagaagcgg ttcttaacga aattgagcgg ttcaaaaaag
     3001 ctgtttcaag ctcggaacac gaactagcaa agataatatc tgataaaaag acccccggtc
     3061 agacaagatc aattcttgaa gttcacagga tgatgcttac agatccgaca gtttcggaac
     3121 ttgtccttaa gactgtgact gaaaagaaga taaatgccga gtgggcggtg cttgatgttt
     3181 tcagcgggct tgttgaaatg cttgaccgga gcagttcagg ttactacatc aaagcaaaga
     3241 tagttgacct tgaagtgata agagacaaac tgatatcaca gttgacagga ggcgtttcac
     3301 ctactgttct tgagatgccg gaagaagatc ttgttatctg ttcgcactct ctgacagtaa
     3361 gcgaactcaa ctatttttct caatttccaa atgttaaagg gatagttctt gaatcaccgg
     3421 gaggtgtcag ccatcttaca gtagttctcc gggcacttga gatccctgcc gtcatgggag
     3481 tcccttcgct tatatcaagc atagattacg gtgacattgt tattgttgac ggagttaacg
     3541 gggaagttgt tctgagacct gacagaagtg agatcaaaga tgcaataagg aaaaacaaaa
     3601 ggtttgaaaa atattttgcc agatttcttg aaaatgttga aacaccctct gtttcaaagg
     3661 atggccgcat actcaaaatc ggcggcaaca ttgatcagcc gggtgaggtt ccgttcgtaa
     3721 agaaatacgg aggcgagttc atcggacttt tcaggactga actcatgttc cctgaaaaac
     3781 aggttgtccc gagtgaagag agacattatg agatatattc gcaggtactt cattttgcaa
     3841 acccgctgag agcgaccata aggatattcg atttcggaga ggataaggaa ggagccatag
     3901 ttcacaaggg atttatgggg atgcgcggca tccggctctg taaaagaaga cccgatgtgt
     3961 tcattccgca gataagggga cttatcaggg cggcggaaca gggcaatctc aacattcttc
     4021 tcccttttgt aagcaatgtg catgaagttg atgattttaa ggagcttctg ttcaaggagg
     4081 cggcaaagat ggggcttagc cgcaatcttg acaatgtcga gctcggtgcg atgatagaag
     4141 tcccttcagc gatattcatt gccgaacaga tcgcaaaaga ggtcagcttt ttcagtgtcg
     4201 gaactaatga tctcattcag tatcttatgg ctgtcgaaag gaaagatcag tattcatcag
     4261 attatttttc ccattttcat ccttcagtca tcagggttct tttcaatctt gcccatattg
     4321 caaaagagca ggggaaagag atctcgatat gcggtgaaat ggccgctgac agatattttc
     4381 cgttagtatt tcttgcgatg gggatatcgt ctcttagcat gagccctatc tcaattccga
     4441 tagtaaagaa gatcattaag tgcggctatg taaaagaagg cgatatgatg ctgaaaaaaa
     4501 tgcttgtagc aaaaaagaac agtcaaataa ttaaaattat gagagatttt atggttgaca
     4561 gatatccaca catatttacc gaaacatgga acaacaatta aaaaggaggc agaaatgaca
     4621 ttcaaatcga tttccagttt tattgaaatg gcaaaagccg gtgaaaaaag gaaaattgct
     4681 gttgcggccg cggaagatga gccagtcctt atggctgtga agaacgccat tgccgaaggg
     4741 atcgttgagc cgatccttgt cggagacgaa aaacagatca gagagatttc ggcaaaaata
     4801 gatttttctc ttgacggaat agagataatc aacgaattaa agcccgctaa tgcatgcagg
     4861 aaagcggtgg cgcttataaa agaaaagaag gcggggatac ttatgaaagg tcttgtaggc
     4921 actgcggatc ttctgaaggc ggtacttgac aaggagaacg gtctcagaaa aggatcggtg
     4981 ataagtcata tcgcattttt tgaaagccct tattaccaca aggttttcat tgttacagat
     5041 gcggcaatga atgtggctcc ggaatttaag gaaaaggttg caattgtaga aaatgcagta
     5101 gatgcctgcc accgtctcgg acttgaaaaa ccgaaagttg cagtaatagg cgcagtagag
     5161 gttataaatg agaaaatgga agcaaccgta catgccggaa tgcttacaat gatgaacaaa
     5221 aggggtcaga taaaaggttg cataatcgac ggtcctttcg ctcttgataa tgcagtttca
     5281 aaagaagcgt gtcatcacaa aaagatagaa acagaagtgg gcggtgatgc cgatattctc
     5341 atgtgtcccg atatcgaagc ggcgaatgtt ctctataaat ctcttaattt ccttggcgga
     5401 gctgaatcgg cagcggtcat tatgggagcc agcgttccga tagttcttac aagcagagcc
     5461 gatagcgaaa agagcaaact taattcgatc gcgcttgccg cggcaatgaa atagggaggt
     5521 tgtaatgaaa atacttgcaa taaacccggg atcaacctca acaaaaatag cagtttttga
     5581 agatacgaaa gagattatga cacagaacat aaagcacaca actgaagagc tgaaggattt
     5641 ttcccatatt tttgannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnttgaa
     5761 ccagaaagct gtcggaagaa aatatgcaag agaaaaaaat aagaaatatg aagaacttaa
     5821 tctaataatt gcacatcttg gcggaggtat ttcggtcggt gcacacaaaa aaggtaaagt
     5881 tgtggatgta aacaatgctc tgaacggtga agggcctttt tcacctgaaa gaagcggcgg
     5941 aattccttca ggacagctta caaaactctg tttctcagga aagttcacct cagcagaaat
     6001 tcacaaaaag ataaaaggcg aaggcgggct tgtagcatat cttggaacga acaatgccta
     6061 cgaagtcgag aaaaaggtgg ctgcaggtga taagcacgct gaactcattt accgtgcgat
     6121 ggcatatcag atctcaaagg agatagggct ccttgcaacg gttctgaagg gtgaagtcga
     6181 tgcaataatc ctgaccggcg gcgttgcata tgacaaggga tttgttgcat ggataaagga
     6241 gcatgtaagt ttcatcgctg aagttgttgt atatccgggt gaagatgaaa tgacagcgct
     6301 tgcggaaggt tgctattacg gcataaaagg agaaataact gttagagatt atgaataggt
     6361 cttgtttact attctttttt atactggctg ttataagcgg atgcagtgat accgttataa
     6421 aaaaggatct tctcaaaccc ggtttttatg aattgtgcta cagtttcccc aaactgcctg
     6481 aaaatacaaa cgaaaatgac ataagaacag cttctgaagc atacgaggaa catttaaccg
     6541 gcaacgggat aacggttctc aaaagaaggt atgcggctga ttctgtctgc tactttacag
     6601 attccatcaa agatctttct cttccccgcg tagagcttga gaacaatatt tcacagtatc
     6661 tcctcacggc ctatttcaat agtgagcata gttcacttta cggagttttc caggggaaga
     6721 ggagatcgga aaagatcacc gacaacatac cgtttttaag aaaacttccg atatatattg
     6781 aaaaacagga aggtcttgta atatcaggga ctaacaggaa aatgcttttt gtggcgaggt
     6841 atcttgatgt tcttgagata gagaatgaat tcggcttcaa tgacaaaggg agaatggtca
     6901 tcagtgtcag atctgacgaa tcagagaggt ggaagatgat accgttcctt ttcgatcttc
     6961 tcaatctcga tgaatttgta tcacttcttc cgggtgatta tgcggttctg aacgaatgca
     7021 gagaggaaaa agtctctctg aagctcagca taccggcatg ggcggtttat tctgtgcctg
     7081 ttgcaaaaga caaaggtgtc gtaaatgaag atgccctggt cttttctgtc agctcgatgt
     7141 cgcttccatt tgatgaagag acgctgctga agtattttta ttcactttca aagagctatc
     7201 ttcttcctcc ttcgagcgga ctttactact cacctaactt taaaggttca aatgtgagtc
     7261 cgacagttct tcttttttca tcggaacttg ccatatcggg ggaatacatt atttctcccg
     7321 agatgatata tatgtgggtc tcttccgtaa aaacccagct tgaacagttc cctgattctt
     7381 tctatgcgaa tcttcttgca cttaaaattg caagaaagct ttatacaggt actccttctt
     7441 ttctgggcgg atctgagctt gtgaacaaaa ctacggcagt cccttttgaa aatattgcaa
     7501 agatgctttt tcacacagga aatgtgactg ttttcggctc catcagcagc tcgctatacc
     7561 tttataacgg agacattata agcgagttct ccaatttgat agaaatgcag aaggtcgcaa
     7621 gagtgaaagc ccttttcggt tcacccgaaa acagcgaccg ctccataatc tcatttgtcg
     7681 taagatcggc aaatgcggaa gtgcttaccg gcaggatagg ggatgatctg aaaaaagagg
     7741 gatttgatcc gacacatctt ctgtttgagc aggtctcatt gaatgatgca tggggatcgg
     7801 tttctgtttc tgttgataaa aaaagtgaaa agaagatcct tgacctgtat gaaaaaaagt
     7861 ttaaattatt aaacaacaca atgtcaatcg gagtttaccg tgtcgcaaaa gaagaatgat
     7921 gctgttaaaa aaaagaaaaa gggctccggt gaaggatcaa gcgaaaaaaa actgatcatt
     7981 attgaaatat ctgccgcgtt tgtctttctt tctgccatat tcctgtggtt ttctctggcc
     8041 aatttttcgg caaagtcggt taggagcggg ttctttacag gcaccctggg gtatgatgcc
     8101 gcacactcgc tgataaattt attcggaata gtttcgtttt acatcccttt acttcttgct
     8161 cttacaatac tctgtttcat aaagagaggg tttataaggc atgtgatact taactacttg
     8221 aatttttttg ttcttctccc tttgttctgg atgatatccg gttatttccg cttttacggg
     8281 attaaaaatg aaaattttct cgggaagctc atatatgaaa acattttcca tttatatctg
     8341 ggtaaaacag gttttatcct tctgacaaca tgctggatga taattttttc aatattttca
     8401 ttcaatgtaa gctatttttt cattttcaga aagatggtgt ggttctggag caggttcttt
     8461 gaaaaagtta aagagacaaa actgatagat agagatgatc cggggcaaga tgatattgat
     8521 gaaaatgacg gcgttgaaga agaactgaca gaaaattcag atgacagaaa agagttcagt
     8581 tttgagccgc cgccggttcc ggaaaagatt gaagaaaaga ttgaaaaagt tgttgaagaa
     8641 aagaagatca ctaaaaggaa agatgatatt tcggtcagca tcgcagagca ggacggtaca
     8701 ggacttgaaa gaaaaactgt aagatcgcat cctgattatt ccggctataa attcccgccg
     8761 cttgatcttt tgaaaggcga agaaaaagta aaagctgaaa atgacagaga gaagaaggtc
     8821 agggaaagag cactgcttct tgaaaggaag cttcttgaac acagacttaa ggttgcagtt
     8881 cagggagcaa cagtaggacc tgttgttaca atgtatgaag tggagctggg tgaaggtgtg
     8941 agagtgaacc aggtaatggc aatggagact gatctcggtg tcgctgtcgg agggcgaaag
     9001 ataagggtgg tttcaagtct tccgggaaaa ccgtacatcg gcatagaagt tcccaatgat
     9061 gaccggctga tgatccggct caaaactatc ttaagaagcg atatgttcgt taaacagagc
     9121 aaaacagggc ttcctatcgc tcttggtcag gatgtatcag gcaagccgtt cgccgcaaat
     9181 ctggtaaaaa tgccccatct tcttgttgca ggtacgacag ggagcggaaa gtcggtcggt
     9241 atcaacacat ttattgtttc tctgcttttc agtttaaagc cggatgaagt taagttcatc
     9301 atggtcgatc ctaaaggtaa tgagttcaat atttatgaag gaattccgca tctccttctt
     9361 cctgtggtcg tagacagcaa aaaggcggca aaggcgcttc aatgggcggt aaatgaaatg
     9421 gaagagagat tccaccgtct tgcagagaat atggtgcgtg acatcgaggc atacaatgaa
     9481 aaggttgaca acataaacaa aaatcttgcc aatgttgagc accacatgaa aaagatgccc
     9541 tacattgtcg ttgtaattga cgagttcgct gatctgatga tggttgcggg aaaggatgtg
     9601 gagatcgctg tttcaagaat agcacagaag gcgagggctg tcggaataca tcttattata
     9661 gcgacacaga gaccgacaag ggatgttgta actgggctta tcaaaggaaa tctgccggta
     9721 agaattgctt tcagggttgc gtcagcactt gattcaagaa cgatactcga ttccaacggt
     9781 gccgaaatgc ttcttggtaa cggtgacatg ctttacattc cgccaggatc atcggaagct
     9841 gaaagagttc acggtgcttt tgtcggtaca gatgaaataa aaaagatagt taaattcatt
     9901 aaggatcagg tcggtgatga tgttgcgacg gatgattcga tattaggttc ttcgccgatc
     9961 gattttcttg aagatgaagg tgaaggcgga tatggaggcg agaacgaaaa ggatccgatg
    10021 tacgatgaaa tactcgccta tgttctttct accggaaagt gcagtgcaag catgcttcag
    10081 agaaagttca aaatagggta taacagagcc gcaagggctg ttgaagtgct tgaggcgcaa
    10141 gggatagttt ctccggctga cggagccaaa cccagaacgg ttataaaccc gaaagagggc
    10201 tgatcagccc tttcttaaat gatgtttcct taagaatgag tcaactgctg aatacatgca
    10261 gggtatgagg acaagtgtta caagtgttga aaatgtgagt ccgaaaacga ctgcgacgcc
    10321 catcgggttg tatgtttcgt gtccttctcc gctcatcatc gcaagcggta ccataccgca
    10381 gatggtcgtt gtggctgtca tgaggatcgg tctgagcctt ctttcacctg aatagatcag
    10441 agcttcatcc atggtgaagt tatgtttcac cttcatggtg ttcgtatagt cgataagaac
    10501 tatcgcattg ttgacgacaa ttccgacaag tatgatcata cccaggaatg ccggaactga
    10561 aagtgcatgc cccgtcagca gaagtcccat caggacaccg cttattgcaa acgggatgga
    10621 gaaaatgata atgaacgggt cgacaaaaga ttcaaactgc gccgccatga caagatagac
    10681 aagcacgata ccgagaagaa gcatgaggac gaggtcgccg gtagtctctt tctggctctg
    10741 tatgtttccg ccgtatatta tttctacatc cggaggaatg tttgaatttt taattgcctt
    10801 ttcaacttcg actgcgatct tgcctatggc tatattgtct tcaagtcttg ctccgatcgt
    10861 gacgatcctt tcgcggttct gtctgttgac cgaaagagat gtcagtccct ccttgacctc
    10921 tgcgacatcc ttcagtttta cagtcgttcc catcagagtc tttatttcga gttctttaag
    10981 ctgttcaatg tttgttctgt cggctttttt atatttgacc attatttcga actcgtcacc
    11041 gtcctttctg tagacagatg ctgtgttccc gtaaaatgca tttcttaccg ccgttcctat
    11101 catcgatgtt ttcaggccta cctgacttgc ccttatgcgg tctatcttga cctcgatctg
    11161 aggtttgaga ctgtcagaat catttgaaat gtccttaagc ccggggatct tttcaagttc
    11221 tttctgaagt ctcagtgcgg cttcatttat ttttgtgaaa tctttgtgag cgagtttaac
    11281 tgtgacaggt ttttcaccgc cactcataag gttgctcatt ccgcttgatg tctgcatatc
    11341 cattttctca atgccgggca cttcttttac tattcttgca cggatcttgt ttacgaacac
    11401 ctttacattc tctttcctgt aatcctgtga aacaagcctt gctcccacag tcgcagtgtt
    11461 cgtatcatca actttctgtg aaaaaccgga atagtcagga cctcctctca taaatgtctt
    11521 aagaagatat ttctgaggga tctcctcttt cattatcctt tctatgtgct gtgctgtagc
    11581 aagagtcgtt tctatttttg taccgagagg catttcaaac gctatctgta tcataccttc
    11641 atcaccctca ggcatgaaat ctatgccgat aatagggatg aaaacaaggg aaacaataaa
    11701 aatgatccca gcggcaagaa ctgttttttt cctgtgtttg agggtggata ctattacttt
    11761 ggtgtataat ttttctgtat aagtaagaaa tttttctccg gccctgaaaa accagttgtc
    11821 ataattggtg ttcgtctctc ttgtcaggat ctttgcgctt accatgggtg taaggagcat
    11881 agcggcaaga agagaagcgg tcaaagtaag gattatgaca ccggcaagct gtccgaaaag
    11941 gattcctata agacctccga caaagaagag cggagcaaaa attccgatcg ttgtaagggt
    12001 cgatgcaagt atggctccgc cgacctctga agttccgtcg atcgcagcgt caatcttgtt
    12061 tttaccatcg tccagatggc gcataatgtt atcaagtgcg acaattgagt tgtcgacgac
    12121 cattcctata gcaagggaaa gcgacatcag cgacaccatg ttcagagtga acccgaatcc
    12181 gtacaggaac gcaaaagcga tgatcatgga agccgggatg gaaagaagta cgataagact
    12241 cgacttaacc ctgcgcagga agaatatgac gacaagaatg acaaagaaac acgctgtcag
    12301 gacagaggat gcaaggcttg agatcgtttt cttgatgaaa tctgaagtat caaggagtat
    12361 gtctatctta aatccgtccg gaagtctggg ttttatctct tcaagttttg ctgtgaccgc
    12421 atcagctatt tcgacagtat tcgcgcctga ctgtttctgc accatcatga aaaccatata
    12481 ttcgccgtct ttcagaccga actgtcttgt atcttcggcg ccccagaaaa cccttgctac
    12541 atctttgatc ttaatgagtc ctgacggggt gcttgcaacg atcgtctcgt tgatctcatc
    12601 tatgttctta tactctccgg gaacccttat ggtatagtcc agagacccta tctctatgtt
    12661 gcctgcagga agcgaaaggt tctctgcctg tacggtgctc acaaggtctg atatggtcag
    12721 attatatgcg gaaagtctct gtttctcagc ggaaataatg atcttctttt ccattctgtt
    12781 gaatgttatt acagatccga caccggggat cctttttatc ggatcagtaa gtgcgtcttc
    12841 aatttcttcc gttttaaatc tgatattttc tttttttgaa gttaccgaaa ggaacattat
    12901 cggcatcatg ctgaaatcga acttgaagat caatggttcc tcaacatcat caggcagata
    12961 tgatcttgac atgttaagaa gatctcttac cttgtttgtg gcttcgctca gatcggttcc
    13021 gtagtcaaat tcacatgtga ctgtggaaac attctccatt gaacggctgt aaatgttttt
    13081 catccctgca gttgaagaaa gagccgattc gatgactttt gaaaccttct cttcaacctc
    13141 ttccgatccg gcacctttgt atatagttat taccgaaatg gcagggatct ccatgtcggg
    13201 catgagatcg atgggcagtt ccttaagtga gtagagaccg aaaagaccca ttatgatgaa
    13261 gataatggat acaaataccg gtctgcttac tgcaaatttc gggaagttca ttttttcacc
    13321 tcactttccg cagttgcatt gtctgaaatg acattgacct tctggccttc ctcgagcctt
    13381 cttcctccgc cgacgactac atcttcgctg ccgtcaagac ctttttcaat tattgaataa
    13441 ccttcgaccg atccgccgat gaacacttct tttgtgaaga ccctgtcagc ttcgttcttt
    13501 gcatagatta taccggcttc attatctctt gtgaaaacgg catttctcgg cacgaccaga
    13561 tattcatgtt cgggaagggt gaacctgact cttactgtca ttcctgactt gaaaacattt
    13621 cccggattgt caatgagcat ttcaaccttt atgcttttgg tcatggtgtc aactttactg
    13681 tttatgagat tgactctggc agtcacttct ttttcgggaa aagcatcgaa tatcactttt
    13741 gcagtagtgt tcttatctat tccgcctatc tcaagttcgc ttatgaaaat ttcgatcttg
    13801 agcttgtcgg tctgaacgat ctcaaacatg ggtctcgggt tggctggtga tgccatttcg
    13861 cctatctcac cgttccttga tgcaatatat cctgaaaaag gtgctatgat cgatgtatag
    13921 ttgaactgtc ttgtattgcc gtcaagagcg ctctgagcca tatttttctg agcctttgcc
    13981 gcggcaagac ctgcctcagc ctgtttatat cctgattcga cctgttcgaa ttcgctgtca
    14041 gaaacagaac cattctgttt aagagttttg aacctttcgt aatctgcgct tatttttgca
    14101 aatgccgctt cagcctgcat tacaccggct tcagccgcgc tgagttgagc ttttgatgca
    14161 ttgactgcga tctcataatc actgccgtca agctgagcaa gtgttgatcc ggcattaaca
    14221 aaatcacctt cttttacatt gaaggatttt attgtagccg caactttcgg gtttatgtag
    14281 gcggatttaa cagccattgc aacagaggcc gtgtcaaaag acctgctgaa ctttcttttt
    14341 tcaggttttg taatcttaac agcctgagtc agattggaac tgtcggtgag ctcatttttt
    14401 gaacatgata caagccctgt tgtaagtgtc agaactatca cagcagcggc ggaaacaagt
    14461 ttagctttca tttatcattc tccatattga agattgctct tttaaggttg ttaagtcctc
    14521 caatgaggaa tttgaatatg tcagtattgt caatattgtc gattctccac ggaaaatcgg
    14581 agatgagtga atatgaggcg agtgctttgg actgtttggt tatgttgcac gacatcgtaa
    14641 caaaggagtg catgaaaccg acgaacatca gcgttatttt ctttgttctt tcgtcatccg
    14701 gactttcacc gatgtattct gcgatcacgc tgttaagcca gttaagttcg ctatcacggt
    14761 ggtccatgat cttttttgct atatcaggtg agttgaacat atatttctgt atctcaagaa
    14821 aaacctctgt tgatatttcc atcagaggtt taaactcttc atacccgccg aaaaacactt
    14881 tgaactgaaa atcgcagtag ataaaaagga tgtctttcgg tgatttggct ttttcaatga
    14941 gaggaacgag tttttcctgt atctttttat tgaaatgttc aaatccctca attagaagaa
    15001 cttcttttga ttcaaagtaa taataaagtg cagattttgt aagccccgat ttttcagcga
    15061 catcgttcag tgtcactctg tttattccgt gctgctggat aagcgacacg aagttcatga
    15121 tgatcatttc ctttttttct tcacttgttc tggccattat tcacctcctg aaacgatcgt
    15181 ctgatagtca actccgacag ttatcgcaag ccggttaagc gcaaccagat gctcatacat
    15241 tgaagtaagc agtttgagct ttgccgatct gagcgatatt gatgcatcaa gcaggtcggt
    15301 ctcggtggtc attttctcac ggtacttatc ttcctcaatt cttaaatttt cagttgctga
    15361 agcgatctcc cgttttgcaa cttcaatcgc ctgatatttg gtttcgacat cattttcaag
    15421 ctgaatgagc tgaagtccga gcatcttttt cgtgttaatg ttgtcaagat cggttttcag
    15481 ccgctctttt tttgccttgt agtattcaaa accggcctta ccccagtcaa aaccgacatt
    15541 ccagctcaac gatccgccta tgaacatcgt cattgcgggc tggaactgag aatcatccca
    15601 tgtcttttta aaaccgcctg caatggcgag tgtagggatg aacggctgtg cggacatcca
    15661 ctccatgtgc ttagctatct tagagctttt ttcgatcatt ttcagttcga tcctgttgtt
    15721 aatcatcata ttttcaagct ctgatgcagt tgatgaaagt gcggcttcaa ccggcttaac
    15781 tgttttgaga gtaaaagaat caaccggcat gttcatgaat attgaaagat tcttctttat
    15841 gagatcaaga ttcccttcaa ctatctgaat ttgctggtca agtcgtgcta tttcgatgtc
    15901 tattttaaga accgctcttt tatcgcttaa gcccgcattg acgaaatttg atgcctgatt
    15961 gtaatatctt ttaagctgtt ccttagtttc ggaaagaagt ttattagatt cggaaagcat
    16021 ctgataattg tagaaaaggg tcatgacttc aagttttatc tgttcgatgg aaagttcctt
    16081 ttgaagtttt gagatttcaa acccgatctc tgccgccttg tatccgttga aaacactcca
    16141 gagcggtgtt atcggttgga cgatcgtcat tcctacagtt ctgcttttat caggaatcgc
    16201 accaaattct acaactccca tccccattgc agtcaggtcc attgtaaccg gttcaggctc
    16261 aggatcgaat ttcagccatt gataacttgc ttcaagcttt gctgtgggaa ggaatgagaa
    16321 aattaccgca ttcttccctg ctttggcggc ttcagctcca gcttcagcag acttgatttt
    16381 ttcgctctct ttgacagaat tttcaataca ttcatcaagt gacagctcac ttgaaaaaat
    16441 aaagaaagga acaaaaaata aaaaagaaat caataatttg cgcatgacac agaacctcca
    16501 gctttcaaac atttgaacaa aaaacaaaaa aagtcaaaaa gttttatatt caaagattca
    16561 aaaatgtcaa gagggagaga aaactttatt ttcaattgta tccagtcact tgattaattt
    16621 tggaattt
//
LOCUS       DAOOYB010000037        16435 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_3454_length_16435_cov_13.100488, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000037 DAOOYB010000000
VERSION     DAOOYB010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 16435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16435
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3454_length_16435_cov_13.100488"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1607)
                     /locus_tag="PL056_03615"
     CDS             complement(<1..1607)
                     /locus_tag="PL056_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46140.1"
                     /translation="MRRIISVFGLLFILLISFTVSAQNKVVVVSTIGKSGAEKKLARE
                     IDDNLSLLFSTIKDADVVGEKDALSSAQSAKITRCNGNLKCIVQVASDAEGVDYIVTT
                     RVQARKGDGVKIAVSLYDSGMKRLGNKPVTAGDDADGEDLASDIIGVVKSLISDNVSS
                     GSSSRSRSSSSSSSSSKKLSSYTEVKNEIVKGFNAYDKGDMDEAADIFNRAANEMKCN
                     CSQNDTAKSLSDDIDKIRKGLPKANDAMNANDYRTALRTLEDVRKADENIREQGYKYM
                     VFKKDRNIRLKYLQPNPKDAEAVEQIHRGFKSKIEEARKWKAKQLLDIDKWVNDNIKE
                     REKKIADTKGREKEILKNQKDAEDALKKKIQDMKYQWEKDDSSLETEIVNLESQITQI
                     EQREKGVIKVSNKKREAEKDKELKTHDKAYADWKTALQKEKDAFYDKQKDLEKVEGDR
                     ITKKVSELEKKKQELEAKNKEIDAEIQKMVEEFEKDERKIMADNESVKMKNEDEDRKY
                     NVTVEKEYQKRFDELNQNLAKYDSQES"
     gene            complement(1775..2881)
                     /locus_tag="PL056_03620"
     CDS             complement(1775..2881)
                     /locus_tag="PL056_03620"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPM46141.1"
                     /translation="MSGYLFSNPQIKKWKTMFFLSFLMNLILIAVIVMREQEVSENTQ
                     ENEELVVKPDSSQDQADVQPDKNSDAGEVQKPENKPAKSNYVPGEPLKGFVVIEENFF
                     SAFSQSEQVAAFASDTGISNLSNLLSAHIGRLLMWDMVLRNDVRKKDRIDFVFRVIPD
                     DEKKKRDDMPDLIEIIAVKYNSKKFNKDIRAYRFLPEKTGFYKYYYKDGTMLEKIISP
                     FPPIKKYFQVTSLLDDRAPRHEGIDYKALVGSPVYSTVEGTVSRKNWMTKYNGYCIEI
                     EEKGKPFVYKYLHLAEVLVDSGQKVKPGDHIANSGNTGKTTAPHLHYQINLGQKGKAI
                     DPFLHHKTVIEKLSGSDEEKFRNVVVDTDRILNL"
     gene            complement(2878..4539)
                     /locus_tag="PL056_03625"
     CDS             complement(2878..4539)
                     /locus_tag="PL056_03625"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HPM46142.1"
                     /translation="MKISETVKLKDVFIMTATGFAVFFYIFLTSHLVGFVRDEGFYMK
                     AASIKADWFEFMEESFSKGDLLEPFSKKSIDRYFRYNNEHPTLIKTMFGFSQYLFERK
                     LGWMSFAQSARFVAALFAALTAVMIYLSGLLFFNRFTAVVSPVMFFLMPHIFFHSHLA
                     CFDVPILFFWSGMFILYAIHLRKKDWLSGIITAVFLGLAMAAKHNVFFIPVILFMSWV
                     TFYFIYYRKEPSAKGLSGFFKTIPKVYYMFVFITIPVYFLSWPWIWYDTVGRFIEYLN
                     FHAKHVNYTNYYFGTELSRGPFPFSFPWAMTFFTTPLPQLIFFISGIYYLSGKVLTEQ
                     VRNEKEINFIFIAGSLFPVFLIALPSVPIFGGIKHWFTGYPLMMTAGIFYISSKMRKV
                     AGKTELFKTVAVSIIFFFSAVSLIPLNVKFAKRGAAFYNQLIGGAQGAAELRMQRNFW
                     GYDILELVGELNRRAPERSTVFVMGGYEGLNWNSFLYLKKEGIIRSDINGTNNLKNAD
                     FAFFFYEKQNEHMLNQIALEFGSAKALAVSETDSVFYSALFGREK"
     gene            complement(4539..5360)
                     /locus_tag="PL056_03630"
     CDS             complement(4539..5360)
                     /locus_tag="PL056_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46143.1"
                     /translation="MSDRSGVDEETEKAFAGVLNSKFEQDDIVFTEVDWDLGFLKYID
                     HGIHPVYLTLKETTAEDLRLMKEDGGKIFFLLKNKDNWKEISDRTGVKEIEIIKAGAG
                     VVVIGSDGTESQRKPLVFSRDIGNAKNVWFSKGDEKFPCTKSSSSKWQCGKDEWNYVG
                     LTTAAMNGKQQRAVWAHPKTRMTLHVEFDVPEKGERIFLNTAFLETGYRSQNSSPVDV
                     ELLMDGASALNYSNKSEKRVYSNSIAIPENSNVLELKFTTEDAGQRHFVFNGYIR"
     gene            complement(5441..6283)
                     /gene="nadC"
                     /locus_tag="PL056_03635"
     CDS             complement(5441..6283)
                     /gene="nadC"
                     /locus_tag="PL056_03635"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HPM46144.1"
                     /translation="MKCPEYVSPDFIKLVINEDIGRGDITTSLIENSGRKGSAVIKAK
                     EDQIICGVDLGAHIFGAIDSSIKVKINVSDGDEISAGTVIMTVSGSSGSILKAERTVL
                     NFLQRLSGIATLTSKYVKQIDRKSKTKITDTRKTTPGWRTLEKYAVRIGGGSNHRFGL
                     DDGIMIKDNHIAMAGSIDKAVRSVRSKAHHLLKIEVETSNLTEVKEALKSKADVIMLD
                     NMSVDMMSKAIELIDKKAIIEISGGVTLEKINELSKLGADFISVGAITHSAVAKDINL
                     TLKI"
     gene            complement(6280..8955)
                     /locus_tag="PL056_03640"
     CDS             complement(6280..8955)
                     /locus_tag="PL056_03640"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HPM46145.1"
                     /translation="MVNMEISKKYDHSGIEKKWYRFWEESGFFKADPESTKPPYSIVL
                     PPPNVTGVLHMGHAVMAVVQDVMSRYKRMSGYDVLWLPGTDHAGIATQMVVERDLQKK
                     GISKHDLGREKFLEKVWEWKNENGNTITTQLRCLGTSLDWSRERFTMDEMLSKAVREV
                     FVSLYEEGLIYRNNYIINWCPRCHTALSDLEVDHEDVEGNLWHIKYPVAGNPDEFLVV
                     ATTRPETMLGDTAVAVHPDDKRYGHLAGKMVELPLTGRTIPVIADSILVDMEFGTGAV
                     KVTPAHDFNDFETGKRHNLEEINVIDEDGLINSLGGKYQGLSTAEARKKIVEDLKEQG
                     LLLKIDKHSHAVGHCDRCRTVVEPILSKQWFIKIESLAKPAIEAVETGRIVFQPDNWK
                     KTYYEWMYNIKDWCISRQLWWGHQIPAWYCEDCGETIVSRVDPVKCTKCSSSNISRDK
                     DVLDTWFSSALWPFSTMGWPEHTKDLDKYYPTTLMETGFDIIFFWVARMIMMGMKFMK
                     DIPFQKVYFHGLVRDEHGQKMSKTKGNVIDPLEITEKYGADALRFTFAMMPLSSRDIK
                     LSVDWIEGYWGFINKIYNAVRFCLMQFKDGEILEFDPVEYKEDDFSFADKWILSRLEI
                     VTAEVNKTLEEMRFNDSAGAVYHFFWNEFCDWYIELVKPAFFGTNEKEKEVSKKVLVK
                     VLDASLKLLHPFMPHVTEELWQNLPFKNSGKLSSIMISRFPRVGDSPAFEKDAAVMEQ
                     IIEIITCIRTIRSENNIKPSLEMSIMLSLNGEVESAIKEKEEYIKKLARVSAIDYVSE
                     NYSPDRETASAMVKGGMIFISLSGLVDFTEEIARIEKEIVKIQKDFDLCDRKLSNPGF
                     LEKAKPDVIEKEKEKHGELKENLEHLKNRLVEIKK"
     gene            9118..9822
                     /locus_tag="PL056_03645"
     CDS             9118..9822
                     /locus_tag="PL056_03645"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HPM46146.1"
                     /translation="MDFFLTGKGRVGTALISYFKSKNIALNDLDESDPDVTGILFAAL
                     PDDHLLNYINKIRNKNNHLHIVHFSGSLTANIERCYLFHPYASINKDTDISDILFTLW
                     GKKNPTLEAALSKTGLKFIYAGTRPTPFYHISAVLSGNFTQFFFVAALELLKQQGFSH
                     NDSIILIKQLIASSIENCVVSATSGITGPAARGDRNTVDAETAVIAGKDEKLAEVFSL
                     INNLIFKAVRNGAVFK"
     gene            9803..10714
                     /locus_tag="PL056_03650"
     CDS             9803..10714
                     /locus_tag="PL056_03650"
                     /inference="COORDINATES: protein motif:HMM:NF013514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HPM46147.1"
                     /translation="MVLFSNNVALSEFVERAYAALLTRKFSVIPESFKEIDYGISFKL
                     DCAEEKKSISVSIYHTEKKGFSFVTKNPDVRSILNSLLTSTGVAGSDEAGKGDLFGPL
                     VVCSFLLGEKENELLKLNIKDSKKLKNHEIIDIYRHISKEHPSSFSLVRIMPERYNSF
                     YKDLAGKGRNLNYMLAWAHSKALSGLIEKRSDIKKIIVDRFTENPVVNKIITDAAGKI
                     PVEFFVRAEQNPAVAAASIIARANYLISLKNISETVLLGKFELISGSGSGSDELLAKI
                     IDEFGTDITDRICKTHFANLTKNLSVF"
     gene            10798..12711
                     /locus_tag="PL056_03655"
     CDS             10798..12711
                     /locus_tag="PL056_03655"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HPM46148.1"
                     /translation="MEENKTDYKLPRLFGKYYLLELINVGGMAEVFKAKMFGVEGFEK
                     IVAIKKILPEVAEDAEFIKMFIDEAKIAVRLQHPNIVQILELGKIDDSYFIAMELVNG
                     KDLKTIRKRLKRVELLMPVEQSAYIISQVCDGLDYAHRKTDDKMNSLNIVHRDISPQN
                     MIVSYEGTVKLIDFGIAKAKSKSTKTQAGMLKGKFSYMSPEQVSGQPIDRRSDIFSLG
                     VVFFEMLTGKRLFLGKNDVETLEKIRKADVPPPSVFNSNVPPELDRIVLKALSKDREE
                     RYQWASEFSEDLKKFSFTTGNSFSRQDMMNFMSEFFADELEEETAKLEEYQKIQKPVD
                     HHQPQHYNDYRQPSRTEKTHHDTEMSMPKRSKKVPVLIAVLLVLSFLAYFGFTMLETK
                     KDTATILIDSSEKNTIVMLNEGTMYQKRCETPCKIEGILPGDHQIELTKSGFVPVKET
                     VSLRQGEVFNKSIKMFKIGEQKTMITVKTDPEGAVLYVNDVKAKALSPTVVDDIPVGL
                     DIVIRIEKQGYFPYEENIGKVPAKVNKEISIILKPMKNAPAIPLQTNRKAEKNKPSVN
                     AKEQAFISINSRPWANVYINGKLVKATPIIKHSLPAGSYKIQFKNPKFNIDKTFPVDL
                     KPGEHQRMIKNFD"
     gene            12820..14085
                     /locus_tag="PL056_03660"
     CDS             12820..14085
                     /locus_tag="PL056_03660"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPM46149.1"
                     /translation="MAPKTKISESLNGNPENGDNNKRPRLSTTTSMLQKSATLEIAAK
                     AKRMNAQGLNVISFSAGEPDFNTPQNVKNAGKEAIDLNFTRYTDSSGTPELKCVIRQK
                     FLRENNLHYDENSIIITNGAKYAIHTLLAAITSPGDEIIIPAPYWVSYPTLVRLNGGV
                     PVFCYAKEEAGFRLTLEDIKPVVTDKTKAIMINSPNNPTGAVYTLKDLEEIVNYLTER
                     NIIVISDEIYEKVIYDNIQHISLASIVKEKYRRNVVVVNGVSKSYAMTGWRIGYAAGP
                     RDIIERMKYFLGHTTSNANSIAMHAAVEALSGSQNSVEMMVKRFNRRRRFMYDELSSI
                     PNLTSAYPEGAFYIFTNFKHYLNKKVNEHKINSTVDLANYLLDEAHVAVVPGESFGMP
                     GYIRFSYATSMDNIAEGMDNLKSALADFE"
     gene            complement(14122..14316)
                     /gene="rpmB"
                     /locus_tag="PL056_03665"
     CDS             complement(14122..14316)
                     /gene="rpmB"
                     /locus_tag="PL056_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HPM46150.1"
                     /translation="MSRKCEICGKKPMFGHNVSHSNNKTQKIQNPNVQKVKIKTENGS
                     SKKTVVCTRCLRSGKVQKAL"
     gene            complement(14406..15347)
                     /locus_tag="PL056_03670"
     CDS             complement(14406..15347)
                     /locus_tag="PL056_03670"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004162.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HPM46151.1"
                     /translation="MRIYHNLKDVPKNEKISLAIGNFDGVHQGHLQIIEKCRKNGGSC
                     TLLTFDVHPREYLYPGFAPRLLTLKHEKYAFLKHAGVDIVIELPFSQYCSIDPIKFLG
                     ILDRELQLDSITIGFNFFFGQNQKGNADLMFWWGRSTGVRINVVPPIIKNGMRVSSTI
                     VREFLATGEMWNAFSFLAYPYVISGKVIEGRRIGRTIDFPTLNLDVPPKIIPPEGVYF
                     TRTVLENETFFSLTNIGANPTIDSEEKTKRIETWIANGNIDEMYGRNIAVYFYKKVRD
                     EIRFSSKEKLSEMINHDRVQLEQFKNANKFEDLPDIF"
     gene            complement(15356..16360)
                     /locus_tag="PL056_03675"
     CDS             complement(15356..16360)
                     /locus_tag="PL056_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HPM46152.1"
                     /translation="MCAVSYKELGLVNTVEMFKKAVDGGYAIPAYNFNNMEQLQAIIM
                     ACVETNSPVILQVSKGARDYANATLLRNMAKGAVEYAHELGKDIPIVLHLDHGSDFEV
                     CKQCIDNGFSSVMIDGSHLPYEENIALTKKVVEYAHSQKDYVTVEGELGVLAGIEDDV
                     VAAHSTYTQPSEVIDFVSKTGVDSLAISIGTSHGANKFKPEQCTRNADGILVPPPLRF
                     DILEEIEHKLPGFPIVLHGSSSVPADLVEMVNKYGGKMKDSVGIPEEQLRRAAKSAVC
                     KINIDSDGRIAMTAAIRKVLAEKPSEFDPRKYLGPAREALKEVYKHKNLNVLGSAHNG
                     "
BASE COUNT         4747 a         3581 c         3084 g         5023 t
ORIGIN      
        1 tcagattcct gggaatcata ttttgcaaga ttctggttga gttcatcaaa tctcttctga
       61 tattcttttt caactgttac attatatttt ctgtcctcgt cctcgttttt cattttaaca
      121 gattcgttat ctgccattat cttgcgctcg tccttttcaa actcttccac cattttctgg
      181 atctcggcat ctatctcttt attttttgcc tcaagttcct gctttttctt ttcaagttca
      241 gaaacctttt tggttattct gtcgccttca accttctcaa gatctttctg cttgtcatag
      301 aaagcatcct tttctttctg taatgcagtt ttccagtcag catatgcctt gtcgtgagtt
      361 ttcagctcct tgtccttttc ggcttccctt ttcttgttgg aaaccttgat aacacctttt
      421 tctctctgct ctatctgtgt tatctggctc tcaagattaa ctatttctgt ttcgagactt
      481 gagtcgtcct tttcccactg atacttcatg tcctgaatct ttttcttaag agcatcctct
      541 gcatctttct gattttttaa gatctctttt tccctgcctt tcgtatcagc aatcttcttt
      601 tccctttcct tgatgttgtc attaacccac ttgtcaatgt caagaagctg tttcgctttc
      661 cattttctgg cttcttctat cttagattta aagcccctgt ggatctgttc gacagcttca
      721 gcgtctttcg gatttggctg aagatatttt agacggatgt ttctgtcctt cttaaagacc
      781 atgtatttat agccctgctc tctgatgttc tcgtcagctt ttctgacatc ttcaagcgtc
      841 ctgagggcag ttctgtaatc atttgcgttc atcgcatcgt ttgctttggg aagccccttt
      901 cttatcttgt caatatcatc agaaagagat tttgccgtat cattctgtga acagttacat
      961 ttcatttcgt tcgcagcacg gttgaatatg tctgctgctt catccatgtc gcctttatca
     1021 taagcattaa atcccttcac gatctcattt ttcacctcag tgtaggatga gagcttcttt
     1081 gagctgctgc tacttgaaga agatgatctt gaacgagaag aagaaccgct tgaaacattg
     1141 tctgaaataa gtgactttac aacaccgatg atatctgaag cgagatcttc accgtcagca
     1201 tcatcgcctg cagtaacagg cttgtttccg agtctcttca tgccgctgtc atagagagaa
     1261 accgctatct tgaccccgtc tcctttgcga gcctgtaccc gggttgtcac tatatagtcc
     1321 acaccttctg catctgaagc cacctgcacg atacatttga gattaccgtt gcatcttgtt
     1381 atctttgcag actgcgctga agaaagagca tccttttcac cgacgacatc agcgtccttg
     1441 atagttgaaa acaacagtga aagattgtca tcaatttctc ttgcaagttt tttctcagct
     1501 ccgcttttac cgatcgttga gacaacaaca accttgttct gagcactgac tgtgaaagaa
     1561 atcagcagaa tgaataataa tccgaaaaca ctaatgatcc ttctcactgg accctcctgt
     1621 caaacgatat gataaagtaa caataaaaca tccactaaaa tcttagtgca tgttaggata
     1681 taaatcagac ggcactttgt caattttttt gtgttataat tagctaaatt tatttaataa
     1741 gataaaacat gccaaaaaaa gacatcggtg aactttaaag atttaaaatg cgatctgtgt
     1801 caacaacaac atttctgaac ttttcttcat cagaccctga aagtttttcg ataacggtct
     1861 tgtggtgaag aaacggatct atcgctttcc ccttttgacc gagatttatc tgataatgga
     1921 ggtgcggagc ggtggttttt cctgtatttc ccgagtttgc aatgtggtct cccggtttga
     1981 ctttctgacc cgaatcgaca agaacctcgg caagatggag atatttgtat acaaacggtt
     2041 ttcccttctc ttctatctcg atgcaatatc cgttatattt tgtcatccag ttctttcttg
     2101 aaacagttcc ttccactgtg gaataaacag gggagccgac aagagctttg tagtcaatgc
     2161 cctcgtgtct gggagctctg tcatcaagca gactggtgac ttgaaaatat ttctttatcg
     2221 gcgggaacgg cgatattatt ttttccagca tcgtcccgtc tttgtaatag tatttgtaaa
     2281 aaccggtttt ttcaggaagg aacctatatg ccctgatgtc tttgttgaac ttttttgaat
     2341 tgtatttgac ggcaattatt tctatgagat cgggcatatc atcccgtttt ttcttttcat
     2401 catcaggtat cactctgaaa acaaaatcta ttctgtcttt tttcctgaca tcattccgga
     2461 gcaccatatc ccacataaga agtcttccga tatgggcact taaaagattt gaaaggttag
     2521 atattcctgt gtctgacgca aaagccgcca cttgttcact ctgactgaaa gcggaaaaga
     2581 aattttcctc tatcacaaca aatcctttca gcggttcacc cggcacataa ttgctttttg
     2641 cgggtttgtt ttccggtttc tgcacttcac cggcatcgct gtttttgtcc ggctggacat
     2701 ccgcttgatc ttgcgagcta tcaggtttta caacaagctc ttcgttttcc tgtgtgttct
     2761 cagaaacttc ctgctccctc atgacaatga ccgcaataag gatcaggttc attaaaaagg
     2821 aaaggaaaaa catcgttttc cattttttta tctgaggatt ggaaaaaaga tagccgctca
     2881 tttctccctc ccgaaaagag cagaataaaa aaccgaatcc gtttcagaaa cggccagagc
     2941 ttttgccgag ccgaattcta aagctatctg gttaagcatg tgttcgttct gtttttcata
     3001 aaagaagaat gcaaaatcgg catttttcaa attatttgtg ccgtttatgt cacttcttat
     3061 gataccttcc ttttttaaat aaagaaaaga gttccagttt aatccttcgt atccgcccat
     3121 tacaaaaaca gtcgatcttt ccggtgctct cctgttaagt tcaccaacaa gttcaagtat
     3181 gtcataaccc cagaaatttc tctgcattct aagttcagcc gctccctgtg ctccgccgat
     3241 taactgatta tagaatgcgg cacctctttt tgcaaatttt acattgagag ggattagtga
     3301 aacagcagaa aaaaagaaaa taattgaaac agcaactgtt ttgaataact cagttttacc
     3361 tgcaaccttc cgcattttgg aactaatata gaaaatgccg gctgtcatca tcagcggata
     3421 acctgtgaac cagtgtttta ttcctccgaa aattggaact gacggcaatg ctataaggaa
     3481 taccgggaaa agagaaccgg caataaatat aaagtttatc tctttttcat tgcgaacctg
     3541 ttctgttaaa acttttccgg acagataata tattcccgaa atgaaaaata taagctgggg
     3601 aagaggtgta gtgaaaaatg tcatagccca cggaaaactg aacgggaaag gacctcttga
     3661 tagttcagtc ccgaaataat aattggtata gttcacatgt tttgcatgaa agttaagata
     3721 ttcaataaat ctgccgacag tgtcatacca gatccagggc cagcttaaga aataaacagg
     3781 aatggtaata aaaacaaaca tatagtaaac cttcggaatt gttttgaaaa acccggacaa
     3841 accttttgca gagggctctt tacggtaata aatgaaataa aaagtaaccc acgacatgaa
     3901 aagtattacc ggtatgaaaa agacattgtg ctttgccgcc atcgccagac cgagaaaaac
     3961 tgcggttata atacctgaca gccaatcttt ctttcttaaa tgtatggcat aaagaataaa
     4021 cattccgctc cagaaaaaga gtatcggcac atcaaaacat gcaagatggg aatgaaagaa
     4081 aatatgaggc atcagaaaga acatgaccgg tgaaactaca gcggtgaacc tgttaaaaaa
     4141 caaaagtccg gaaagatata tcatcacagc cgttaaagca gcgaaaagag ctgctacaaa
     4201 tcttgcagac tgcgcaaaac tcatccatcc gagctttctt tcaaaaagat actgggaaaa
     4261 accgaacatt gtttttatga gtgtggggtg ttcattgtta tacctgaaat atctatctat
     4321 cgattttttt gaaaacggct ccagcaaatc tcccttgctg aaactttctt ccataaattc
     4381 aaaccagtcg gctttaattg atgcggcctt catataaaaa ccttcatctc tgacaaatcc
     4441 gacaagatgt gatgtcagga aaatataaaa gaataccgca aatcctgtcg cggtcattat
     4501 aaaaacatct ttcaatttca ccgtttcaga aattttcatt acctgatata tccattaaaa
     4561 acaaaatgtc tctgccccgc atcttcggta gtaaacttta actccagaac attgctgttc
     4621 tctggaattg caattgagtt ggaataaact cttttctcgc ttttatttga ataattcaaa
     4681 gcacttgccc cgtccatcaa caattcaaca tcaacaggtg aactgttttg tgaccggtat
     4741 cctgtttcaa gaaatgctgt gttaagaaat attctttcac ctttttccgg cacatcaaat
     4801 tcgacatgaa gagtcattcg ggtcttaggg tgtgcccaga ccgctctctg ctgttttcca
     4861 ttcattgcag ccgttgtaag accgacataa ttccactcgt cctttccaca ctgccatttt
     4921 gatgaagatg actttgtgca gggaaacttt tcatctcctt ttgagaacca cacatttttt
     4981 gcatttccga tatcccttga aaaaacaagc ggctttcttt gagattctgt tccgtcagag
     5041 ccaatgacca caacacctgc tccggctttt attatttcaa tttcctttac tccggttctg
     5101 tccgatatct ctttccagtt atctttattt tttaaaagga aaaatatctt tccgccatct
     5161 tctttcatta aacggagatc ttctgccgtt gtttctttta atgtaagata taccgggtgg
     5221 ataccgtgat caatatactt gagaaatccg aggtcccagt caacttcggt aaaaacgatg
     5281 tcatcctgtt caaactttga attgaggaca cctgcaaacg ctttttcggt ctcttcgtca
     5341 acacctgatc tgtcactcat aaacgaccag aaaataaagg ttgaaggaat ggaaataagg
     5401 aacagaatta caaaagatct gccgattatt ttatctatgt tcatatttta agagtaaggt
     5461 tgatatcttt tgcaacagcc gaatgggtta tcgctcccac agaaatgaaa tcggcaccca
     5521 gttttgaaag ttcgtttatc ttttcaagag taactccgcc tgaaatttca atgatcgctt
     5581 ttttatcaat taattcaatg gcttttgaca tcatatcaac agacatattg tcaagcatta
     5641 taacatccgc ctttgattta agtgcctctt tcacttcagt aagatttgaa gtttcgactt
     5701 ctattttaag aagatggtgc gcttttgaac ggactgatct gaccgcttta tcaattgatc
     5761 ccgccattgc aatgtggttg tcttttatca ttattccgtc atcaagtccg aaacggtgat
     5821 ttgagccgcc gcctattctg accgcatatt tttcgagtgt tctccacccg ggagttgtct
     5881 ttcttgtgtc tgttatcttt gttttggact ttctgtcgat ctgttttaca tattttgatg
     5941 ttagcgttgc tatgccgctc aaccgctgaa gaaagttaag aactgtcctt tctgctttaa
     6001 gaattgagcc ggaactgccc gagacggtca ttattacagt ccctgctgat atttcatcac
     6061 cgtcactgac atttatttta acttttatag aactgtcaat agctccgaat atatgagctc
     6121 caaggtcaac tccgcaaatg atctggtcct cttttgcttt aatgaccgca ctcccctttc
     6181 tgcctgaatt ttcaatcagc gaagtggtta tgtcacctct tccgatatct tcattgataa
     6241 ccagttttat aaaatcgggt gaaacatatt ccggacattt catttcttta tctcaacaag
     6301 tctgttttta agatgctcaa gattttcctt gagttcgcca tgcttttcct tttctttctc
     6361 aatgacatcg ggttttgctt tttcgagaaa accggggttg gaaagctttc tgtcacaaag
     6421 atcgaaatct ttctgaattt taactatctc tttttcaatt ctggcgatct cttcagtaaa
     6481 atcgacaagc ccggatagag aaataaatat cattcctcct ttaaccatgg ccgatgccgt
     6541 ttccctgtcc ggggagtaat tttcagaaac atagtctatt gccgaaactc tcgccagttt
     6601 ttttatatac tcttcttttt cttttatagc agattcaact tcaccgttta aagaaagcat
     6661 tattgacatt tctaaagagg gtttgatgtt gttttccgac cttattgttc ttatgcaggt
     6721 tattatttct atgatctgtt ccattacggc ggcatctttt tcaaatgccg gcgaatcgcc
     6781 gaccctgggg aatctgctga tcatgatcga agagagcttt ccgctgttct taaacggaag
     6841 attctgccac aattcttctg taacatgcgg catgaaagga tgaagaagct tcaaacttgc
     6901 atcaagcact ttcacaagaa cctttttgga aacttccttt tctttttcgt tagttccgaa
     6961 aaatgccggt tttacaagtt caatatacca gtcgcagaat tcgttccaga aaaagtggta
     7021 gacggcaccc gcactgtcgt taaacctcat ttcctcaagg gttttattaa cttcagccgt
     7081 tacaatttca agtctggaaa gtatccattt gtcggcaaac gaaaagtcat cctctttata
     7141 ttctacaggg tcaaattcca gtatctcgcc atccttgaac tgcatcaggc agaatctgac
     7201 agcgttatat attttgttta taaatcccca gtaaccttct atccagtcaa ctgaaagctt
     7261 tatgtccctt gatgaaaggg gcatcattgc aaaagtgaat ctcaaagcgt cagcaccgta
     7321 tttttcggtt atctcaagag gatcgatgac attccctttt gtctttgaca ttttctggcc
     7381 gtgttcatct cttacgagcc catgaaaata gactttctga aaagggatgt ctttcatgaa
     7441 tttcatcccc atcatgatca ttcttgcgac ccagaagaaa atgatgtcaa agcccgtttc
     7501 catcagggtt gtaggataat atttgtccaa atcctttgta tgctccggcc atcccattgt
     7561 tgaaaagggc catagtgctg aactgaacca tgtgtcgaga acatccttgt ctctgctgat
     7621 atttgaagaa gagcattttg tgcactttac aggatcaact ctcgaaacga tcgtttcgcc
     7681 gcaatcctca caataccatg cggggatctg gtgaccccac cagagctgac ggcttatgca
     7741 ccagtctttg atgttgtaca tccattcata atatgttttc ttccagttgt ccggctgaaa
     7801 aactattctg ccggtttcaa cagcttcgat cgcaggtttt gcaagggatt ctatctttat
     7861 gaaccactgt tttgaaagta tcggttcaac gaccgttctg catctgtcgc agtgtcctac
     7921 ggcatgtgaa tgtttgtcaa tttttaaaag aagcccctgc tctttcaaat cttcaacgat
     7981 cttttttctt gcttcagccg ttgaaagtcc ctgatatttt cctccaagag aatttatcaa
     8041 gccgtcttca tcaattacat ttatctcttc aagattgtgt cttttccctg tttcaaaatc
     8101 gttaaaatca tgcgccggag tgaccttcac cgctcctgtt ccgaattcca tgtcaacaag
     8161 aatgctgtct gcgatgaccg gaatggtacg accggttaaa ggaagttcaa ccatcttccc
     8221 tgcaagatgt ccgtatcttt tatcatcagg gtgaacagcg acagcggtat ctccgagcat
     8281 agtttcaggc cttgttgtcg caactacaag aaattcatcc ggatttcctg caacgggata
     8341 ttttatatgc cagaggtttc cttcaacatc ttcgtgatcg acctcaagat cgctgagcgc
     8401 cgtatgacat ctcggacacc agtttattat gtaattgttc ctgtatataa gcccttcttc
     8461 atataaagag acaaaaactt cacggaccgc ttttgaaagc atttcgtcca ttgtgaatct
     8521 ctctctggac cagtctaaag aagtgccgag gcatcttaac tgagttgtga tcgtatttcc
     8581 gttctcattt ttccattccc acaccttttc aaggaatttc tcccttccaa gatcgtgctt
     8641 tgatatccct tttttctgca gatccctttc gacgaccatc tgggtcgcta tccccgcatg
     8701 atcagtcccc ggaagccaga gaacatcata acccgacatt cttttgtatc tgctcattac
     8761 atcctggaca acagccatga cggcatgtcc catatgaaga actcccgtaa cattcggagg
     8821 tggaaggact attgaataag gaggttttgt gctttcaggg tctgccttga aaaaaccgct
     8881 ttcctcccag aatctgtacc attttttctc aatacctgaa tggtcatact ttttactgat
     8941 ttccatattg acaattctcc aaataacaac cagttaaatc aatgcgcctt aatttctacc
     9001 tgctaaggga aaaaagtaaa gtttttcaat tgatagacat gctttcgaca atgagtttaa
     9061 atttgactta gcaagggtaa actactaata tcacaatgtt tgacacggga ggaaaaaatg
     9121 gacttttttc tgacaggaaa agggcgtgtg ggaacagctc tgatttcata tttcaagtca
     9181 aaaaatattg cacttaatga tctggatgaa tctgatcccg atgtgacggg cattcttttt
     9241 gcggcacttc ccgatgacca tcttctgaac tatataaaca aaatacgcaa taaaaacaac
     9301 cacctgcaca tagttcactt ttcgggttca ctgactgcga acatagagag atgctatctt
     9361 ttccaccctt atgcttcgat caataaagat actgacattt cagacattct tttcactctc
     9421 tggggaaaga agaaccccac acttgaggcg gccctttcaa aaaccggtct caaattcatt
     9481 tacgcaggga caagaccgac tcctttttat catatttcgg ctgttctttc gggcaatttc
     9541 actcagttct tttttgttgc ggcgctggaa cttcttaaac agcagggttt ttcacataat
     9601 gacagcataa ttctgataaa acagcttatc gcttcatcca ttgaaaactg tgttgtttca
     9661 gccacttcag ggataacggg ccctgccgca agaggtgaca ggaacactgt cgacgccgaa
     9721 acagcggtca ttgcgggaaa agatgaaaaa ctggcggaag tgttctctct cattaacaat
     9781 ttgatattta aagcggtgag aaatggtgct gttttcaaat aatgtggcgc tttctgaatt
     9841 tgtggaaagg gcctatgcgg ctttacttac aagaaaattt tctgtgatcc ccgaaagttt
     9901 caaagagatc gattacggaa tttcgttcaa gctcgattgc gctgaagaga aaaaatccat
     9961 ttccgtatca atttaccaca cagaaaaaaa agggttctct tttgttacaa aaaatcctga
    10021 tgtgcggtcg atacttaact cacttctcac ttcaaccgga gttgcaggaa gcgacgaagc
    10081 tggcaaaggc gaccttttcg ggcctttagt tgtctgctct tttctgctgg gtgaaaaaga
    10141 aaatgagctt ctgaaactca acataaaaga cagcaaaaaa cttaaaaacc atgaaattat
    10201 cgatatttac agacacattt caaaagaaca tccttcttct ttttcactgg tcagaataat
    10261 gcccgaaagg tataattcat tttacaaaga ccttgccgga aaagggagaa accttaatta
    10321 catgctggca tgggctcatt ccaaggcgtt gtcaggactt atcgaaaaaa gaagcgacat
    10381 aaaaaaaata atcgttgaca gatttactga aaatcccgtc gtaaacaaaa tcatcactga
    10441 tgctgccgga aagatacctg ttgaattctt tgtcagggcg gaacaaaacc cggctgttgc
    10501 ggcggcatca attattgcaa gagctaacta cctgatctcc ttaaagaata tctcggaaac
    10561 ggttcttttg ggaaagtttg agctcatatc aggaagcgga agcggatcgg atgaacttct
    10621 cgccaagatc attgatgaat tcgggacaga catcactgac agaatctgta aaacccactt
    10681 cgcaaattta acaaaaaacc tgtccgtttt ttaaaccaaa tatttattga catatcattc
    10741 ccgtataact aatatatccc gattacagat tttcgtatga aaagcggtgt gacccttatg
    10801 gaagaaaaca agactgatta caagctgccg agattgtttg gcaaatacta ccttctcgaa
    10861 ctcatcaatg tcggaggtat ggccgaagtt ttcaaagcga aaatgttcgg agtggaagga
    10921 tttgaaaaga tcgtcgccat aaagaagatc cttcctgaag tcgctgaaga tgccgaattt
    10981 attaaaatgt tcattgatga agcaaagatc gcggtccggc ttcagcaccc gaacattgtt
    11041 cagatccttg aacttggaaa aattgacgat tcctacttca ttgcaatgga actcgttaat
    11101 ggaaaagatc tcaagaccat cagaaaaaga cttaaaagag ttgaactcct gatgccggtc
    11161 gagcaaagcg catacatcat ttcacaggtg tgtgacggac ttgattacgc ccaccggaag
    11221 accgatgata aaatgaactc tctcaacatt gttcacaggg acatctcacc tcagaacatg
    11281 atcgtttcat acgaaggaac cgttaagctg atagatttcg gtattgcaaa ggcaaaaagc
    11341 aagtccacaa aaactcaggc gggaatgctg aaaggaaagt tcagctatat gtcccccgaa
    11401 caggtgagtg gacaaccgat tgatcgtaga agcgatattt tctctcttgg tgtagtattc
    11461 tttgaaatgc ttaccggaaa gcgattgttc cttgggaaga acgatgtcga aacacttgaa
    11521 aaaataagaa aggcggatgt tccgccgcca agcgttttca atagcaatgt ccctcctgaa
    11581 cttgacagga tagttctcaa agcactttca aaagatcgtg aggaaagata tcagtgggca
    11641 agtgaatttt cagaagattt gaaaaaattc tctttcacaa ccggcaattc tttttcaaga
    11701 caggacatga tgaatttcat gagtgagttc ttcgctgacg aacttgaaga agagactgca
    11761 aagcttgaag aataccagaa aatacagaaa cctgttgacc atcatcagcc gcagcattat
    11821 aacgactacc gccagccgtc aagaacagaa aaaacgcacc atgacacaga aatgtcaatg
    11881 ccgaaacgga gtaaaaaggt ccctgttctg atcgcggtac tccttgtcct ttcctttctt
    11941 gcctatttcg gttttacaat gcttgaaaca aaaaaagata ctgcaacgat cctcatagat
    12001 tcctctgaaa aaaacacaat tgtcatgctg aacgaaggga caatgtatca aaaaaggtgt
    12061 gaaacacctt gcaagatcga gggaatactc cccggagatc atcagataga gcttacaaag
    12121 agcgggttcg tccctgttaa agaaacagtc tctctcagac agggtgaagt tttcaataaa
    12181 agcatcaaaa tgttcaagat cggcgaacag aaaacaatga taaccgtcaa aactgatcct
    12241 gaaggggcag tcctttatgt taatgatgtt aaagccaaag ctttgtctcc aaccgttgtg
    12301 gatgacattc ctgtgggtct tgacatcgta ataagaattg aaaaacaggg gtacttccct
    12361 tatgaagaga atataggaaa ggttccggcc aaagtaaata aagaaataag tatcatcctg
    12421 aaaccgatga aaaacgctcc ggcgatccct ttgcagacca atagaaaagc agagaaaaac
    12481 aagccctcag taaatgcaaa ggaacaggca ttcataagca ttaacagtcg cccgtgggcc
    12541 aatgtctaca taaacggaaa actcgttaaa gcgacaccca tcatcaagca ttctcttcct
    12601 gcgggaagtt ataagatcca gtttaaaaat ccgaaattca atatcgataa aacctttccg
    12661 gttgatctta aacccggtga acatcagaga atgatcaaga atttcgactg atttccattt
    12721 aaatttatct tgattaaaga ttttttgagc aatatactcg ccccgataca gctctttaaa
    12781 ctataaactt tagtaatttc aatttgatga ggtgtagtta tggctcccaa gacaaagatc
    12841 agtgaaagtt tgaacggaaa tccggaaaac ggcgacaaca ataagcgccc aagattaagc
    12901 accactacct caatgcttca gaaatctgcc actcttgaaa tagcggcaaa agccaaaagg
    12961 atgaatgcgc aggggttgaa tgtgatctct ttctctgccg gagagcccga tttcaacaca
    13021 ccgcagaatg taaaaaatgc agggaaggag gcaattgatc tcaatttcac aagatacaca
    13081 gattcatcag gaacacctga actcaaatgt gttatccgcc agaaattctt aagagagaac
    13141 aatcttcact atgatgaaaa cagcatcatc attacaaacg gcgcaaaata cgccattcac
    13201 acccttcttg ccgcaattac cagccccggc gacgaaatta taattcctgc accttactgg
    13261 gtcagctatc cgacgcttgt ccgtcttaac ggaggagttc ccgttttctg ttatgcaaaa
    13321 gaagaggccg gtttccgtct cactcttgaa gacataaaac ctgttgttac tgacaagacc
    13381 aaggcaataa tgataaactc tccgaacaat cctacaggag ctgtttacac actcaaagac
    13441 cttgaggaga tcgttaacta ccttacggaa aggaacataa ttgtgatcag tgatgaaata
    13501 tatgaaaagg taatttatga caacatacag cacatttcac tggcaagcat tgtaaaggaa
    13561 aaatacagaa ggaatgtcgt tgtagtaaac ggagtttcaa aatcctatgc catgaccggc
    13621 tggagaatag gttatgccgc aggaccgaga gacatcattg aaagaatgaa atatttcctc
    13681 ggtcacacga catcaaacgc aaactcaata gcgatgcatg cggcagttga agcactgagc
    13741 ggaagtcaga actcagttga gatgatggtt aaaagattca accggagaag acggttcatg
    13801 tatgacgaac tcagctccat tccgaacctt acatctgcat atcctgaggg ggctttctat
    13861 attttcacca actttaaaca ttacttaaat aaaaaagtga atgaacacaa gataaactca
    13921 actgttgatc ttgcaaatta tcttcttgat gaagcccatg ttgcagtagt tccgggagaa
    13981 agcttcggta tgccgggata tatccgcttt tcatatgcaa caagtatgga taacattgca
    14041 gaagggatgg ataatcttaa atcagctctt gccgacttcg aataatttaa aacaaatatc
    14101 ttcagaaata ctttcagaaa attacagagc tttttgaact tttcctgaac ggagacatct
    14161 tgtgcagacg actgttttct tagaggagcc gttttctgtt tttattttca ctttctgaac
    14221 attcgggttc tggatcttct gtgtcttgtt gttcgaatgg cttacattgt gaccgaacat
    14281 cggctttttt ccacatattt cacatttgcg tgacatcgtt ctctccttca tatgttaaca
    14341 tcagcaaaaa atcaggtgga tattacatgc tttgggtgta aatgcaaatt tttccagaca
    14401 aagattcaga atatatcagg caaatcttcg aatttattgg cgtttttaaa ctgttcaagc
    14461 tgaactctgt cgtggttgat catctctgaa agtttttcct tagatgaaaa acggatctcg
    14521 tcccttactt ttttataaaa ataaactgca atatttctgc cgtacatttc gtcaatattt
    14581 ccgtttgcga tccatgtctc gatccttttt gtcttttctt cagaatcaat agtcggattc
    14641 gctccaatat tagtcagaga aaagaatgtc tcattttcta aaaccgtacg ggtaaaataa
    14701 actccctcag gtggtattat tttcggcgga acatcaagat taagcgtggg aaaatctatt
    14761 gtccttccga tcctccggcc ttctatcact tttcctgaaa ttacataagg atatgcaaga
    14821 aatgaaaatg cgttccacat ttcgccggtt gcaagaaatt cccttactat ggtcgaactg
    14881 accctcattc cgttctttat tatcggagga acaacattta ttctgacacc ggttgatctt
    14941 ccccaccaga acataagatc tgcatttcct ttctggttct gaccgaaaaa gaagttaaat
    15001 ccgatggtta tcgaatcaag ttgaagttct ctgtcaagta tcccgagaaa ctttatcgga
    15061 tctatcgaac agtattgcga aaacggaagt tcaattacga tgtcaacgcc ggcgtgtttc
    15121 aaaaaagcat atttttcatg tttcagggta agaagccgcg gtgcaaagcc ggggtaaaga
    15181 tactctctgg gatgaacatc aaatgtcaga agtgtgcatg aaccgccatt ctttctgcat
    15241 ttttcaatta tttgaagatg accctgatga acgccgtcaa aatttcctat cgcaagggag
    15301 atcttttcat ttttaggaac atctttgagg ttatgataaa ttctcatgaa ttttctcagc
    15361 cgttgtgggc actgccgagc acatttaaat ttttgtgttt ataaacctct ttaagtgctt
    15421 ctctagccgg tccgagatat tttctgggat caaattctga cggtttttcg gcaagaactt
    15481 ttcttatcgc ggcagtcatt gcgatacggc cgtcgctgtc gatattgatc ttacagactg
    15541 cactttttgc cgctcttcta agctgttctt ccggaattcc gactgaatct ttcatttttc
    15601 caccgtactt attgaccatt tcaacaaggt cggcaggaac tgatgaagaa ccgtgaagaa
    15661 cgatcggaaa accgggaagc ttgtgttcga tctcttcaag aatgtcaaaa cggagaggcg
    15721 gcggaacaag tattccgtca gcatttcttg tacactgctc cggcttgaat ttgttggcac
    15781 catgacttgt gcctattgaa attgcaagtg aatcaacgcc tgtttttgaa acaaaatcaa
    15841 ttacttcgct tggctgagta taagttgaat gagctgcaac cacatcatct tcaatgcctg
    15901 caagaactcc aagttcccct tcaactgtca cataatcctt ttgcgaatgg gcatattcaa
    15961 caaccttctt tgtgagtgcg atattttctt cataaggaag gtgtgatcca tcgatcatga
    16021 cagagctgaa accgttgtca atgcactgtt tacaaacttc aaaatcactg ccgtggtcaa
    16081 gatggagaac gattggaata tcttttccga gttcatgtgc gtattcaact gcacctttgg
    16141 ccatattcct taaaagcgtg gcattggcat aatcccttgc acctttagaa acctgtaata
    16201 tcactggaga atttgtttca acacaagcca tgatgatcgc ctggagctgt tccatgttgt
    16261 tgaagttata ggcagggatc gcatatccgc cgtcaacagc tttcttaaac atttcaacag
    16321 tattcaccaa cccgagctct ttgtaactta ctgcgcacat atcttcctcc tgaaagttaa
    16381 ataagatcaa atccgcaatt agtataatca gcaaaaaaaa attgtgaaac tttta
//
LOCUS       DAOOYB010000038        15964 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_3598_length_15964_cov_11.710164, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000038 DAOOYB010000000
VERSION     DAOOYB010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 15964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15964
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3598_length_15964_cov_11.710164"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2591)
                     /locus_tag="PL056_03680"
     CDS             complement(<1..2591)
                     /locus_tag="PL056_03680"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HPM46153.1"
                     /translation="MVSILKVLYHILSKKRFALFIVFSILVIAAGSAASKIKLEEDIT
                     KAIPFGDDGEEYSRILKEFKFLDRIIFTVSFADRSQDDHMELANFANDFIDGMKKSEC
                     ASMIDSFSNEKTLIDQKAVYSHFYEFLPFFLDENDYAELERNTLPEVLKETVGALYRN
                     IISPAGAVSAQFIRRDPLSITFKGMEKLKSLNPESSFEIIDGHIFADSRKSLIFFIQP
                     ALSAGDTGNNAKLVSEMERVLSETTAKYEGRIKGGFFGGIPVGVGSADRIKTDVIISS
                     VVAVILIFLILMLYFRKLKLIPLVFLPAVFGALAAVSVAVMIKGTISAIALGITSVLL
                     GIMVDYSIHVISHVIKSRNTADAISDLSFPLILSCLTTVAAFVCLLFLDAPLLNDLGL
                     LASIAVLSAMIFSIVILPHIIDAVRSQDDISHCMEKENGLISKLSSIEFEKNRILLIF
                     MVLLTAVLYFYSNDVRFEDNLDSINYIPPELKGTMNEIDRISSINKKAVYLVYQGDSL
                     DKVLEEREKSEFAINKLFSEGVITSISDISGVVMSKSRQAKKIERWNSFWNSERKKEV
                     KETLITEGAVYGFKEDAFADFYELIDKKFDPVDPYWLTKFAPSLFDEWIAESKNGYMT
                     AALLKLSGPDSSEISEYVSGSSDMFLFDRAEIMHRFVKYLKMDFEKLLTYSILVVFLI
                     LFILSGRIELALLSMIPVTFSWIWTLGVMNIFGIKFNIVNIIIVTFIFGLGIDYVAFI
                     MRAKMQEYAHGSKETMISYKSSILISCLTTVAGVGALFGAVHPALRSIALIAVTGMIS
                     TLINSFVVAPFLFDWLMIRQKKKKAPPHTFIVFTYTFLAYANYVIFSLILSSLGFAIF
                     TTWPFKSG"
     gene            complement(2593..3756)
                     /locus_tag="PL056_03685"
     CDS             complement(2593..3756)
                     /locus_tag="PL056_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HPM46154.1"
                     /translation="MWSEKFREIKCCVIIPTYNNSKTLSSVISGVLKFTDSVIVVNDG
                     STDGTSDILKQFQDITVVTHEVNKGKGIALRTGFKKAVELDLDYAVTIDSDGQHNPED
                     LPNFIEKIEKEPGSLIVGARNMEQSTVPGKSSFGHKFSNFWYKVETGIDLPDTQSGYR
                     LYPVKKLKDIRYITNRFEFEIEVIVRAAWTGIRVTHVPVSVIYFPKETRVSHFRPVRD
                     FTRVSILNTILVFLAFTIFRPLMYIRDLKKKSAREIVENILGSNESALKISSAVALGG
                     TIGVSPFWGFQIMLILIFAHILKLNKVTALIASHISIPPFIPFIIYYSYVLGGYFYDS
                     EIALDINNLTLESVSLNIVQYVTGSFVLMVVTGILFFAVTFPVIKIRRKKRTA"
     gene            complement(3865..4380)
                     /locus_tag="PL056_03690"
     CDS             complement(3865..4380)
                     /locus_tag="PL056_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006647485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46155.1"
                     /translation="MIVQTDGYEFDFTDAINAFKFDETNHSSPTFHGVTELKADDIIA
                     ELSGAYLFIEIKEYADHNIFNEALGANSQDKKERHDKYKWLKRYLKYKYRDSFLYRFA
                     EEKVDKPVHFICLLNFDNALNNKMKKDLSRELPVGKKSPRWKKKLVESCQVLNLNQWN
                     LQFPKWPASKI"
     gene            complement(4377..5414)
                     /locus_tag="PL056_03695"
     CDS             complement(4377..5414)
                     /locus_tag="PL056_03695"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPM46156.1"
                     /translation="MIKKIKFEKFTAFDSLDIDFSPGINVFIGENGTGKTHILKTIYS
                     ACDITKSKKGFADKINDVFYPSAKQIGRLVKRSTTSIKGSVEIKRVVKEQEMSLKLSL
                     TNHTMTPKKAKISGTYKQWMETQMESVYIPVKDMMANAAGFRSLYAMRNLHFEEVYAD
                     IIDRAFLPALKGPTENKRRKILDILQQAMDGKVILKNEEFFLKNKQGELEFTLLAEGF
                     RKLGLLWLLIQNGTLLSGSSLFWDEPETNLNPRLMKTVVDILIELQRLGVQIFISTHD
                     YVLLKEFDLAVNSKDNIFYHSLYHNKELNNIIVCSTSKSFDAISPNAIDDTFGSIINR
                     QIEKEMGGLGK"
     gene            complement(5651..6034)
                     /locus_tag="PL056_03700"
     CDS             complement(5651..6034)
                     /locus_tag="PL056_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011771164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HPM46157.1"
                     /translation="MYYIVETEKMFDQASIDLEQAVKRLGFGVLHTHDLGESLRSKGV
                     EFNEQCRIFEVCNPQHAADVLSSDMRLNMVLPCRISVFTQKGKTKIGLIKPEKMLMAL
                     SNDPELVFVAKEVEKKIIQMVDEAK"
     gene            complement(6086..6754)
                     /locus_tag="PL056_03705"
     CDS             complement(6086..6754)
                     /locus_tag="PL056_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic aminobenezylarsenical efflux permease
                     ArsG family transporter"
                     /protein_id="HPM46158.1"
                     /translation="MIAALISAIWLGILTSISPCPLATNIAAVSYISKNLSDKKSVAM
                     SSILYTFGRMAAYTLISAIIVISVVSTPKLSHFLQVWGTKIIGPVLILTGAFLLELIT
                     IPIPSFGGQPVKPEKFGLFGSFLLGFIFALSFCPVSAALFFGSLIPLAVSEQSRFVLP
                     LLYGIGTALPVVLFAILIATGSSKIGTVFTHITKIEKWVRTAFGVILIILGLYYTVMY
                     VFLN"
     gene            complement(6751..7233)
                     /locus_tag="PL056_03710"
     CDS             complement(6751..7233)
                     /locus_tag="PL056_03710"
                     /inference="COORDINATES: protein motif:HMM:NF040494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrophenyl compound nitroreductase subunit ArsF
                     family protein"
                     /protein_id="HPM46159.1"
                     /translation="MDKKKISGIILLIFVALSVIVLIAKEMTKNTSTTEVNVPQKQET
                     QLKRHLKVIYFHSTMRCPSCLKIENYTKETVTSKFKPEMDSGLIEFSEINVDKPENEK
                     YIEQYQLTTKQVIVSEYENGIEKKWKDLDRVWELLREEEEFKSYVEMEVNGWLNEVKK
                     "
     gene            complement(7242..7475)
                     /locus_tag="PL056_03715"
     CDS             complement(7242..7475)
                     /locus_tag="PL056_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HPM46160.1"
                     /translation="MKKLQILGTGCPKCKKLAEVTEAAAKELGIEFELIKVTDINEIM
                     DFGVMMTPCLAVDGTVKVVGKVPSVEEIKKLIG"
     gene            complement(7497..8792)
                     /locus_tag="PL056_03720"
     CDS             complement(7497..8792)
                     /locus_tag="PL056_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011188188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HPM46161.1"
                     /translation="MNWKNEWKPLSIIVSVFFAAFFLPVGVYRFDNALKEALELVKWY
                     AEEHVLLCLVPAFLIAGAIGVFISQNAVMKYLGAGAKKSVAYLVASVSGTILAVCSCT
                     ILPLFAGIYKRGAGLGPAIAFLYSGPAISVLSIILTARILGAEIGIARAIGAISFSVI
                     IGFIMEKIFQKEEAARSAALQMPVAEEGRPLYQTVIYFFFMVMILVFANWGKPDVTEG
                     LWWSIYSVKWIITAVSALLFGVVLVAWYSHKVWKIVLIGIVSAIFAFVFPSIPIIAFS
                     AGFIGLTWSGSTADEENGDWISASWGFAKQILPLLLIGVFVAGFLLGRPGNEGIIPNE
                     WVAGLVGGNSLFSNFFAAIVGAFMYFATLTEVPIVQGLMGSGMGKGPALSLLLAGPAL
                     SLPNMLVIKSVIGTKMTAVFVVLVVIMATLTGLIYGSLF"
     gene            complement(8789..9082)
                     /locus_tag="PL056_03725"
     CDS             complement(8789..9082)
                     /locus_tag="PL056_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013513460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HPM46162.1"
                     /translation="MKTKEDYKREVLILKALANEARLMIVDSLKDRDITVGELTELLK
                     LDQSTVSKHLSVLYNAGIVAYRKDGNLVYYQLLTPCVLDAFKCACRVVKEGKK"
     gene            complement(9166..12636)
                     /locus_tag="PL056_03730"
     CDS             complement(9166..12636)
                     /locus_tag="PL056_03730"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HPM46163.1"
                     /translation="MKGLEKSFKEAALAVIAAENGMISPQKLVHIFSDWVNSPGVSIR
                     KKLEETLPEENRALLDLLGSESVEDEPQNRSIEVLMDSVNRIKEQTRMAVGTPTGQGD
                     NTSDRLITDETPGKYMIQGELARGGAGRVLIALDRHIGREIAMKELLLDLRDKRDGKI
                     RNDPQTVSLRNRFLREARVTGQLEHPSIVPVYEIGRHPDGIFYYTMRMVKGKTLNQAI
                     KICNSLEDRLELLPHFYNICNAVAYAHSKGVINRDLKPSNVMIGEFGETVVLDWGLAK
                     VKGQQDMGAEKLQTGLRLFLDADVGKTVVGYAIGTPSYMPPEQAEGKVDDIDETSDTY
                     SLGAILYQLLTGDPPYSGKTASEILRKVVDTDPQKATSVDRNIPPELAAIAHKAMSKK
                     KKDRYSSVTELIDELNSFMSGGNVKVYEYSSMEILKRFASKNKAVFISSILILVMLSL
                     STLMIAWYYDREVTAKKTAQKEKIVSQYRTAQAFNEKAARLETQKQFLSSRIYAAASL
                     YFNPANRNSPEYVPWFRKNFKDSDVLLSDAASRFYHRHFHRGALLETGFDTDCTITAL
                     AVSSDLSSIFAGCGNGDVAVYSYPGLKEKNRFKTGSQIDSIDLTEDGKFAAFAGISGA
                     VTIADLEKNTVSTLIKKGLGIKSIRFSPDGSQLLSCGIDSIIKIVNLTSGEAVEIKGH
                     AGIVNDAVFVDEKTILSASADSTVRIWDKDAGSEISLYQTDEEVKKLAVINGPGIILA
                     GTENGSVIVLSKVDLRFIRKLSYHGSPVTSIDVSSDGSLFLTSERERKSVVWDAGKMI
                     PLFALEGHRNNMTAAVFDNNSEKIITVGTEGFIRVWARHDRKEIKTFSYDFQKIRKGS
                     ISKNGKLALLPKNDLIVVQDMKEGTRKEIQLQTPVLDVDISPDGSKIAAACWDTIVRI
                     IDVETGSFVNELKGNENAVSAVKFSGDGRYVASAGRDGSISIFSSQGGERIAYKCASG
                     GATGVSFSSDSTLVSGICDNGDIPVLKVPAMEVVKLIKNYEKKPVSVSFTADNGSIMT
                     GYLAHSPEVTGINSDTLISLPGQHGSVTATAVSDDGKFGATSSTDSLVQLWDMAEHKP
                     FLTITTQKDPGCIMFMPDSSSLGVCDGGTIKFYPLSTPDLEMDPLTLLKKMEKEAGMK
                     LKDFYLEAGM"
     gene            12790..15375
                     /gene="mutS"
                     /locus_tag="PL056_03735"
     CDS             12790..15375
                     /gene="mutS"
                     /locus_tag="PL056_03735"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HPM46164.1"
                     /translation="MSAIEKALETPMMKQYTAFKEKYPDAILFFRLGDFYEMFFDDAV
                     TASEILGITLTCRHKDAEIPLAGIPWHSADQYIRKLLEAGKKVAVCEQTEKPDKKKKT
                     VERDVVRILTPGTVVEESSLVENRSNWLMSVFFEKDSAAVSWVDVSCGDVFYTVVNET
                     EIEDVVRNIGPKEIVMNTTSSHICAEVIDQERFEDWVPSENGANYLEKLRDYNILNSV
                     EKSLEMMLFYVDNLYFGNFPPLRTPEKWKSEDSAALDYNTAANLEIEKTLIGGNREGS
                     LLWAIDRTQTPMGKRVLANTIKKPLKNLDMILLRQQCVSTFYSDGTLLRNIREDLGKI
                     RDFERTLSRILVKRGGPREIRMLADSLVFAIGLKDSILKRDDLEFFKSTPAFNITGKI
                     AQNWKKRFVDEPPFNYKEGGFVSPDHSPEMFQLSDLINNSRGHIAALEAREKESTQIP
                     TLKAGFNRVFGYYFEVSKRFADKVPAHYIRKQTTANGERYFTAELKELEEKILTAKDK
                     MTVLELEILEKTVVEINGYKEDILNLAKLISWLDLFCGFAKLAVDNNYCCPDVVEKEG
                     IEIVDGRHPVVEASISKGSYVPATVVIGDAQKRFCIITGPNMGGKSTVMRMTALVVLL
                     AQTGSFVPAKSARIGIVDSIFTRVGASDNLSKGESTFLVEMKETAYILNSATSKSLII
                     LDEIGRGTGTYDGISLARAIAEHIIKKINAVTLFATHYHILTELAEDFSSAFNFHMGA
                     KEYGGKIRFTYQMNEGGSSRSFGVEVAKLTDMPRTVIKTAEKILKEMEMADRKFRFER
                     GGSLQTDIFSMNFGQVETIPDHLHELENMIRRIDPEKMSPREAMNILFQLFDIVNEKK
                     ENENG"
     gene            15368..>15964
                     /locus_tag="PL056_03740"
     CDS             15368..>15964
                     /locus_tag="PL056_03740"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPM46165.1"
                     /translation="MVKVGGNIKAITPYKPGKPISEVERELGLKDSIKLASNENPLGP
                     SEKVVEAVRAAASELNFYPDGNAFYLKNAIVEYHKNRGEEIHFDELIVGNGSNEVIDI
                     AIRTFLSGNEEIIVSEQAFIVYELIPAAANIKTVLIPQTKDHVVDIDAHIAAINDKTK
                     MICLVNPNNPTGTYYSKAAFEKLLKAIPEDVILAVDEAY"
BASE COUNT         4649 a         3449 c         3115 g         4751 t
ORIGIN      
        1 ccgcttttaa aaggccatgt ggtgaatatc gcaaaaccga gcgaagaaag gatcaagctg
       61 aaaatgacat agtttgcata tgcaagaaaa gtgtatgtga aaacaatgaa agtgtgcggc
      121 ggagctttct ttttcttctg tctgatcatc agccagtcaa aaaggaacgg ggcgaccaca
      181 aaggaattta tcagcgtcga gatcatccct gtcaccgcta tcagcgctat tgatctcaat
      241 gcggggtgta ccgctccgaa cagtgctcct actccggcaa cggtggtaag acatgagata
      301 agtatcgaac ttttatagga tatcatcgtt tcttttgatc cgtgggcata ttcctgcatc
      361 tttgctctca tgatgaaagc gacataatca atgccgagcc cgaatatgaa tgtgacgatg
      421 attatattga caatgttgaa ctttattccg aaaatgttca ttactcccag agtccatatc
      481 cacgaaaatg ttacaggaat catcgataaa agggcaagtt caatccggcc cgaaagtata
      541 aaaaggataa gaaaaaccac aagtattgaa taagtaagca gtttttcaaa gtccattttc
      601 aggtatttaa caaatctgtg cattatttca gccctgtcaa aaaggaacat gtcgctgctt
      661 cctgaaacat attctgatat ttcagaactg tcgggaccgg aaagtttaag gagtgcggcg
      721 gtcatgtatc cgtttttgct ttcagctatc cattcatcaa aaagtgaagg ggcaaatttt
      781 gtaagccagt aagggtccac cggatcgaat ttcttatcga taagttcgta aaaatctgca
      841 aaagcatctt ctttaaaacc gtaaactgct ccttctgtga tcagggtttc tttaacttct
      901 tttttccttt cactgttcca gaaagaattc catctttcta tctttttcgc ctgtctgctt
      961 tttgacataa ccaccccgga aatatccgag attgaagtta tgaccccttc gctgaaaagt
     1021 ttgtttattg caaattcact tttctctctt tcttcaagga ctttatctaa cgaatccccc
     1081 tgatagacaa gatataccgc ttttttattt attgaactta ttctgtctat ctcgttcatc
     1141 gttcctttaa gctcaggagg aatgtaatta atgctgtcaa ggttgtcttc aaaccttaca
     1201 tcgtttgaat agaaatacag aaccgccgtc agaagaacca tgaaaattaa aaggatccgg
     1261 ttcttttcaa attcgatcga actcagtttt gatataagtc cgttttcttt ttccatgcag
     1321 tgggaaatat catcctgtga tcttactgca tcaattatat gaggcagtat cacgatggaa
     1381 aaaatcattg ctgaaagtac tgctatggat gcaagaagtc cgagatcgtt aagtaaagga
     1441 gcatcaagga aaagcagaca tacaaatgcc gcaaccgtag taagacagct cagtattagg
     1501 ggaaatgaaa gatcggatat cgcatctgcg gtatttctgc tttttatcac atgggatatt
     1561 acatggatcg aatagtcaac cattatgcca aggagtacgg aagtgatacc gagggctatt
     1621 gcggatattg tcccctttat catcactgcg acagaaactg cggcaagagc tccgaaaacg
     1681 gcagggagga acacaagggg aattaacttc agttttctga aatagagcat aagtataaga
     1741 aatataagga tcaccgccac gacagaggat attattacat cggtctttat cctgtcagcg
     1801 cttcccactc ctaccggaat accgccgaaa aagccgcctt tgatcctccc ttcatatttt
     1861 gctgtcgttt cagaaagcac tctttccatt tcacttacaa gttttgcatt gttaccggtg
     1921 tcacctgcag aaagagcagg ctgaatgaaa aatatgagac tttttctgct gtcggcaaag
     1981 atatgtccgt cgattatctc aaatgaactt tcagggttga ggcttttgag tttctccatc
     2041 cctttgaaag taattgaaag gggatcgcgc cttatgaatt gtgcagatac cgctccggcg
     2101 ggagagatta tgttcctgta aagagctccg acagtctctt tcagcacttc aggcaatgta
     2161 ttcctttcaa gctctgcata atcattttca tcaaggaaga aaggcagaaa ttcatagaaa
     2221 tgtgaataaa ccgccttctg atcaatgagc gttttttcgt ttgagaagga atctatcatc
     2281 gatgcacatt ctgacttttt catcccatca ataaaatcgt tcgcaaaatt tgcaagttcc
     2341 atatgatcat cttgcgatct atctgcaaat gatacagtga atattatcct gtcgaggaat
     2401 ttaaactctt ttaatattct gctgtattcc tctccgtcat ctccgaaagg gatcgctttt
     2461 gtgatatcct cttcaagctt tatttttgaa gcggcagaac cggcggcgat aaccagaata
     2521 gaaaacacaa tgaacagagc aaaccgtttt tttgaaagaa tatggtaaag aacttttaat
     2581 attgaaacca ttttatgctg tcctcttttt tcttctgatc ttaataacag gaaaagtcac
     2641 tgcaaaaaaa agaatgcctg tcaccaccat gaggacaaat gaccctgtaa catactggac
     2701 aatgtttaaa gacactgatt cgagagtgag attgttaatg tccagcgcga tttctgaatc
     2761 gtagaaatat cccccgagaa catagctgta gtaaattatg aacggtatga aagggggaat
     2821 tgaaatatgc gacgcgatga gtgcagtaac tttattaagt ttcagaatgt gtgcaaagat
     2881 cagaatgagc attatctgaa aaccccagaa aggggaaact ccaatggttc cgccaagagc
     2941 tactgcggaa gatattttca atgcagattc gtttgaacca agaatgtttt caactatttc
     3001 acgggcactt ttctttttca ggtctcttat atacatcaac ggtctaaaga tcgtaaaagc
     3061 taaaaaaaca aggatcgtgt taaggatgct cacccttgta aaatcgcgca ccggtcggaa
     3121 gtggctcacc cttgtctctt tcgggaaata aatgactgaa accggcacat gtgtcaccct
     3181 tattcctgtc catgcggctc ttactatcac ttctatctca aactcgaatc tgtttgttat
     3241 gtatctgata tctttgagtt ttttaactgg ataaaggcga tatccgcttt gtgtgtcagg
     3301 aagatctatc cctgtttcga ctttgtacca gaaattggag aacttgtgtc cgaaactgct
     3361 tttgccaggt actgttgact gctccatgtt ccttgcaccc actatcaggg agccgggctc
     3421 tttttctatt ttctcaatga aattgggcag atcttcggga ttatgctgac cgtctgaatc
     3481 aattgtgacc gcatagtcaa gatcaagttc cacagctttt ttaaatccgg ttctaagcgc
     3541 gatccctttc cctttgttca cttcatgggt tacaacggta atatcctgaa actgtttaag
     3601 aatatctgag gtgccgtcag tcgatccgtc attcacgaca atgactgaat ctgtaaactt
     3661 taaaacccct gaaattactg atgaaagtgt tttgctgtta ttatatgtcg gaattattac
     3721 acagcattta atttctctga atttttcaga ccacattttc ccgcccaaaa aaaaacaaga
     3781 ggagtttatt tgcaaaaggg agtgttgtca attttgagaa taattcaagc tcttactggt
     3841 tttattttaa taaagttata tcgcttaaat cttacttgcc ggccactttg ggaactgcag
     3901 attccattga tttagattta atacttgaca gctttctacc aattttttct tccatctggg
     3961 tgactttttg ccaacaggca actcccgact taaatccttt ttcattttat tgttcaaggc
     4021 attatcaaaa ttaagaagac agataaaatg aaccggctta tccacctttt cttcagcaaa
     4081 acgatataaa aaactatctc gatatttgta ttttaaatag cgtttaagcc atttatattt
     4141 atcatgtctt tcttttttgt cttgggaatt agcacctaaa gcttcattaa aaatattgtg
     4201 atcagcgtat tccttaattt caataaaaag atatgcacca gacaattctg caataatatc
     4261 atcagcttta agttccgtta ctccatggaa tgttggtgac gaatggtttg tctcatcaaa
     4321 cttaaaagca ttaattgcat cagtaaagtc aaattcatac ccatcagttt ggactatcat
     4381 ttcccaagcc ctcccatttc tttttcaatc tgccgattga taattgatcc gaaagtgtcg
     4441 tcaatcgcat tcggagaaat ggcatcaaac gatttgctgg ttgaacaaac aatgatgttg
     4501 tttaattctt tgttgtggta aaggctgtga taaaaaatat tatcttttga gttgacagcc
     4561 aaatcaaact cttttaataa tacatagtcg tgagttgaaa taaatatctg aacaccaagt
     4621 ctctggagtt ctatcaaaat atcaacaact gttttcataa gtctgggatt tagatttgtt
     4681 tcaggttcat cccaaaaaag tgaactacca ctcaacagtg tcccattctg aattaaaagc
     4741 catagaagtc ctaactttcg aaacccttca gcaagaagag taaattcaag ttctccctgc
     4801 ttatttttaa ggaaaaattc ctcgtttttc aaaattactt ttccatccat tgcttgttga
     4861 agtatatcaa gaattttcct tcttttattt tccgtaggtc cttttaatgc tggtaaaaaa
     4921 gctcgatcaa taatgtcggc ataaacctct tcaaaatgaa ggtttcgcat tgcgtaaaga
     4981 gaacgaaaac ccgcagcatt agccatcata tctttaacgg gtatataaac cgactccatc
     5041 tgcgtttcca tccactgctt gtatgtgcca gagatctttg cttttttagg ggtcatggtg
     5101 tgatttgtca aagacagttt taatgacatt tcctgttctt ttacaacacg ctttatttcc
     5161 acggatcctt ttatgctggt cgttgacctt tttacaagtc tgcctatttg ctttgctgaa
     5221 gggtaaaaga catcattaat tttatccgca aatccttttt tactttttgt gatatcacag
     5281 gcggaatata tcgttttaag aatgtgagtc tttcctgttc cgttttcacc tatgaaaaca
     5341 ttaattccag gagaaaaatc aatgtcaaga gaatcgaatg ctgtgaattt ttcaaactta
     5401 atctttttaa tcataactct tttacctcaa taaattatgt gacaaaaaag catatgataa
     5461 tataaaaaaa atgccattat atcaagcttt ttaaataatt tgttgtcttt tatctccgtg
     5521 gaaatcagtt cgcagaaatt ttagatagaa ctctttcaga tatttcagga taaaggagac
     5581 ggatacagtc aggacagatc gtatgagaaa catctgtagc tgaaattaaa attcatcatg
     5641 tgacaagtga ttattttgcc tcatcgacca tttgaatgat ctttttctca acctcttttg
     5701 caacaaaaac cagctcagga tcattagata gtgccataag cattttctcc ggtttaataa
     5761 gaccgatctt cgttttgcct ttttgggtaa aaacagagat tcgacacggc aaaaccatgt
     5821 tcagccgcat atccgaagac aatacatcgg cagcatgctg agggttgcaa acctcaaaaa
     5881 ttctgcattg ttcattaaat tcaacgcctt tacttctaag tgattctccc aggtcgtgag
     5941 tgtgaagcac accaaagccc agacgcttaa ccgcctgctc aaggtctatt gatgcctgat
     6001 cgaacatctt ttcagtttca acaatgtagt acattcgttt tcctccttgt cttaaaacat
     6061 ttttcgtaaa acccgcgtct tcctctcaat tcagaaacac atacataacc gtgtaataaa
     6121 gtccgagaat tatcaagata actccgaaag cggttctcac ccatttttcg atttttgtga
     6181 tgtgggtaaa tactgtgccg atttttgagc tgcctgttgc gatcaggatt gcaaaaagga
     6241 caacggggag tgctgtgccg atgccgtaaa gaagaggaag gacaaacctt gactgttctg
     6301 atactgcaag tggtatgagt gatccgaaaa agagtgcggc tgatactgga cagaatgaga
     6361 gcgcgaagat gaatccaaga agaaaagagc cgaacagtcc gaatttttcc ggttttaccg
     6421 gctgtcctcc gaaagatggg atcggaattg ttataagttc aagtaaaaat gcaccggtca
     6481 ggatcagcac gggtcctatt atctttgttc cccatacctg aagaaaatgg gagagtttag
     6541 gtgttgaaac aactgaaatg acgattatag cgcttatcag agtatatgcc gccatccgcc
     6601 cgaaagtgta aagaattgaa gacattgcaa cacttttttt atcactcagg ttctttgaaa
     6661 tatagctcac agccgcaata tttgtcgcaa gcggacacgg tgaaattgaa gtcagaattc
     6721 caagccatat cgctgaaatt aaagccgcga tcattttttt acctcgttaa gccagccgtt
     6781 cacttccatt tcaacatagc ttttaaactc ttcttcttct cttaaaagct cccagactct
     6841 gtcgaggtct ttccattttt tttcaatgcc gttttcatat tctgaaacta tgacctgttt
     6901 tgtagtaagc tgatattgtt cgatatattt ttcgttttcc ggcttatcaa catttatttc
     6961 actgaactca ataagacctg aatccatctc tggcttaaat tttgaagtga ctgtttcttt
     7021 agtataattt tcgatcttca ggcacgacgg acaccgcata gtgctgtgaa agtaaattac
     7081 tttaaggtgc cgttttaact gagtttcctg cttttgcgga acatttactt ctgttgttga
     7141 agtgtttttt gtcatctctt tggcgatcag gactatgacg cttaatgcaa caaaaattaa
     7201 aaggatgatt cctgatatct ttttcttatc catctttccc cctaccctat cagttttttt
     7261 atctcttcaa cacttgggac tttgccgaca actttaactg ttccatccac tgcaagacag
     7321 ggggtcatca ttacaccgaa atccatgatc tcgttgatat ctgtaacttt gatgagttca
     7381 aattcgatcc cgagttcttt tgctgctgct tctgtaactt cagcaagttt tttgcatttt
     7441 gggcagccgg ttcctaaaat ctgtaatttt ttcatagtgt tacctcttaa aaaatattaa
     7501 aaaagtgagc cgtaaataag acctgttaaa gttgccatta tcacaacaag aacaacaaaa
     7561 accgctgtca tttttgtgcc gatgacactt ttaatgacaa gcatattagg cagagaaagt
     7621 gcaggacctg ccagcagcaa cgaaagtgca ggaccttttc ccatgccgct tcccatcaaa
     7681 ccctgaacga taggaacttc tgtgagagtt gcaaaataca tgaatgcacc gacaatcgca
     7741 gcaaagaagt ttgaaaaaag agagttcccg ccgacaagtc cggcaaccca ttcatttgga
     7801 attatccctt cattccccgg tctgccgagc agaaaacccg caacaaaaac gccaatcagc
     7861 aaaagaggca ggatctgttt tgcaaaaccc cacgaagcac ttatccagtc accgttctct
     7921 tcatcagcag ttgagccaga ccatgtcagc ccgataaagc cggctgaaaa ggcaattatc
     7981 ggaatagacg ggaaaacaaa tgcaaaaatc gctgaaacaa tgccgataag aactattttc
     8041 caaaccttat gtgaatacca tgcaacaaga acaactccga aaagcagtgc tgaaactgct
     8101 gtaattatcc attttactga ataaattgac caccatagcc cttcagtcac atccggtttt
     8161 ccccagttag cgaaaacaag gatcatcacc atgaaaaaga aatagatcac cgtctgataa
     8221 agcggccttc cttcctcagc taccggcatt tgaagtgctg cagaacgggc cgcttcctct
     8281 ttttggaata tcttttccat gataaatccg ataattactg aaaaagagat tgctccgatc
     8341 gctctggcaa taccgatctc agctcctaaa atccttgcag tcagtattat cgaaagaaca
     8401 cttatcgcag gacctgaata taaaaatgcg attgcagggc caagacctgc ccctcttttg
     8461 taaatgcctg caaataatgg cagaattgtg catgaacaca ccgcaagaat agttccggaa
     8521 acactggcaa caagatatgc aacgctcttt tttgctccag ctccgagata tttcatgaca
     8581 gcattctgcg aaataaatac accgatcgct cctgctatca gaaatgccgg aaccagacag
     8641 agcagaacat gttcctcagc gtaccatttc acaagttcaa gtgcttcttt cagtgcatta
     8701 tcaaaacggt aaactcccac cggcagaaaa aacgccgcaa agaacaccga aacaataata
     8761 gaaagcggct tccattcatt tttccagttc attttttccc ctccttaaca accctgcatg
     8821 cacatttgaa cgcatcaagc acacaaggag taagcagttg ataatataca agatttccat
     8881 cttttctgta agcaacaatc cctgcattgt aaagaaccga aaggtgtttg ctcaccgttg
     8941 actgatcaag cttgagcagt tctgtaagct cgcccactgt tatatcgcga tctttaagcg
     9001 aatcaacgat catcagcctc gcttcatttg ccagcgcctt taaaatcaga acctctcttt
     9061 tataatcttc ctttgttttc attttgcacc tccgcaaaaa acgcaacatt actatattgc
     9121 caatttgcca tattgtcaag tggttgcttt aactgtttga ttttactaca ttcctgcttc
     9181 gaggtagaaa tccttgagtt tcattccggc ttctttttcc atttttttaa gaagtgtcag
     9241 cggatccatt tcaaggtcag gggttgaaag aggatagaat tttattgttc ctccgtcaca
     9301 gactccaagc gaactgctgt ccggcataaa cattatgcat cccgggtctt tctgggtcgt
     9361 tattgtaaga aacggtttgt gttccgccat gtcccaaagc tggacaagtg aatcggttga
     9421 cgatgtcgca ccgaacttcc cgtcatctga aacagcggtt gccgtaaccg aaccgtgctg
     9481 tcccggaaga gagatcaatg tgtctgaatt tattcctgtt acttcaggcg aatgggccag
     9541 ataccctgtc attatgctcc cgttgtcagc cgtaaaagag accgataccg gctttttctc
     9601 atagtttttt ataagtttaa cgacttccat cgcaggaact ttcaatacgg gaatgtctcc
     9661 attgtcacat attcctgaaa cgagagtgct gtcagaagaa aaagaaactc ctgttgcacc
     9721 tcccgacgca catttatagg cgatcctttc tccgccctga gaagagaaaa tgcttattga
     9781 tccgtctctt ccggcagaag cgacatatct tccgtcccct gaaaacttaa cagcggaaac
     9841 agcgttttcg ttgcctttca attcattcac aaagctgccc gtttcaacat caattatcct
     9901 tacgatcgta tcccagcatg ccgccgcgat ttttgatcca tcgggagaaa tgtcaacatc
     9961 gagcaccggg gtctgaagct gtatctcttt ccttgtcccc tctttcatat cctgaaccac
    10021 aatgagatca ttcttcggaa gaagtgcaag tttcccgttc tttgaaatgg aacctttcct
    10081 gatcttttga aaatcgtatg aaaatgtctt gatctctttt ctgtcatggc gcgcccagac
    10141 ccttatgaat ccttccgttc cgacagttat tatcttttct gaattattgt caaacactgc
    10201 ggcagtcatg ttgttcctgt gtccttcaag agcaaaaaga gggatcattt tccctgcgtc
    10261 ccagacaaca cttttccttt ccctttcaga tgttaaaaaa agagagccgt cggatgaaac
    10321 atcaattgag gtcaccgggc ttccgtgata tgaaagcttt cttataaatc tgagatcaac
    10381 ctttgacaga acaataactg atccgttttc cgttcctgca agaattattc ccggtccgtt
    10441 aattacggca agctttttta cttcttcatc tgtctggtaa agtgatattt cgctgccggc
    10501 atctttatcc cagatcctta ccgttgagtc cgccgaagca gatagtattg ttttttcatc
    10561 aacaaagacc gcatcgttaa ctattcccgc gtgacctttt atctcaacgg cttctcctga
    10621 agttaaattt actatcttaa ttattgagtc tattccgcag gaaagaagct gtgacccgtc
    10681 aggagaaaat cttatagatt tgatccctaa accctttttg atcagtgtcg aaacggtgtt
    10741 tttttcaaga tcggcaattg tcaccgcacc cgaaatacct gcaaatgcgg caaactttcc
    10801 atcctctgtc agatcaatgc tgtcgatctg tgagccagtc ttgaatctgt tcttttcttt
    10861 taatcccgga tatgaataaa cagcaacatc cccgtttccg caacctgcaa agatcgatga
    10921 caaatcactt gaaacagcaa gtgcagtaat tgtacaatcg gtgtcaaacc ctgtttcgag
    10981 cagggctcca cgatgaaaat gcctgtgata gaaccttgaa gcggcatcac ttaaaagaac
    11041 atcggagtct ttgaaattct ttctgaacca cggaacatat tccggactgt tcctgttggc
    11101 gggattgaaa tagagcgatg ctgcggcata tattcttgac gaaaggaact gtttctgggt
    11161 ttcaagacgg gccgcttttt cattgaatgc ctgtgccgtc ctgtactgcg aaacgatctt
    11221 ctccttctgc gcagttttct tggctgtgac ctcccggtca taataccatg ctatcataag
    11281 tgtcgaaaga gagagcatta caagaatcag tattgaggaa atgaaaactg ctttgttctt
    11341 tgacgcaaac ctcttgagta tctccattga agaatattcg taaactttta cattgccccc
    11401 cgacatgaaa ctgttcagct catcaataag ttccgtaacg gaagaatacc tgtccttttt
    11461 cttttttgac attgccttgt gcgctatcgc cgcaagctca ggaggaatgt tgcggtcaac
    11521 agatgtcgcc ttctgcggat cggtgtctac aacttttctc aatatttctg acgccgtttt
    11581 tccggagtac ggcggatctc ctgtcagaag ctgataaagt atggctccca gagaataagt
    11641 gtctgaagtt tcgtcaatgt catcaacttt cccttccgcc tgttcgggag gcatgtatga
    11701 cggagttccg atcgcatatc ctacaacagt ttttcctaca tcggcatcaa gaaaaagacg
    11761 gagtcctgtc tgaagctttt ccgctcccat gtcctgctga cctttaactt ttgcaagacc
    11821 ccagtcaaga acgaccgttt caccgaactc cccgatcatg acatttgacg gtttaagatc
    11881 gcggtttatg acacctttgc tgtgggcata cgccactgca ttgcaaatgt tgtaaaaatg
    11941 gggcagaagt tcaagccggt cttcaagcga attacatatc tttattgcct gattaagggt
    12001 ttttcccttg accattctca ttgtgtaata aaaaattccg tccgggtgtc tccctatttc
    12061 gtaaacagga actattgaag ggtgttcaag ctgtcctgtg acccttgcct ctcttaaaaa
    12121 cctgttgcga agactcactg tctgaggatc gttccttatt tttccgtccc ttttgtcacg
    12181 aaggtccagc agcagttcct tcattgctat ttcgcggccg atgtgtctgt caagggcgat
    12241 cagaactctt ccggctccac ctcttgcaag ttcgccctgt atcatatatt ttccaggagt
    12301 ttcatcggta atgagccggt ctgaagtgtt gtctccctgc ccggtcggag tgccgacagc
    12361 cattcttgtc tgttccttta ttctgttgac gctgtccatc agcacttcaa ttgatctgtt
    12421 ctgtggctca tcttcaacag attccgaacc gagaagatca agaagagccc tgttctcttc
    12481 aggaagtgtc tcttcaagct tttttcttat cgaaacaccg ggggagttca cccagtctga
    12541 aaagatatgg accagcttct gtggagatat cattccattt tccgcggcga taacagctaa
    12601 agccgcctct ttgaacgatt tttcaagacc tttcattgct ttccctttca gcaaattatt
    12661 caaatcacga cattatatgt gttttttttt aaaaacaaaa aaattattgt tctgtgtcta
    12721 tcttctttat tttttatttt ttgtatgata tggtcaaagc ggtgtcggcg gttttataca
    12781 ggtgaatgaa tgtcagctat cgaaaaagct cttgaaaccc ccatgatgaa acaatatacg
    12841 gcgttcaagg aaaaatatcc cgatgcgata ctttttttcc ggctcggtga cttttatgaa
    12901 atgttctttg atgatgctgt aactgcttct gaaattttgg gaattacact cacctgccgt
    12961 cataaggatg ccgaaatacc gcttgccgga attccgtggc acagcgctga ccagtatatc
    13021 agaaaacttc ttgaggcagg gaaaaaagtt gcagtatgtg aacagaccga aaaacccgat
    13081 aaaaagaaaa aaacggtcga aagagatgtt gtaagaattc tcactcccgg aactgtggtt
    13141 gaagaaagtt cgctggtaga aaacaggtca aactggctta tgagtgtgtt ctttgaaaag
    13201 gattctgcgg ctgtttcatg ggtcgatgtg agctgcggag atgtttttta caccgttgtg
    13261 aatgaaacgg aaattgaaga tgttgtcaga aatataggcc cgaaagagat cgttatgaac
    13321 accacttcgt cacatatttg cgcagaagtg atagaccagg aacggtttga agactgggtt
    13381 ccttctgaaa acggggctaa ctatctggaa aaattgagag attacaacat tttgaacagt
    13441 gttgaaaaat ctcttgaaat gatgcttttc tatgttgaca atctctattt cggcaatttt
    13501 ccaccgctta ggacccctga aaaatggaaa agcgaagatt cagcggcact tgattataac
    13561 actgcggcaa atcttgaaat agaaaagaca cttataggcg gaaaccgtga aggatcgctc
    13621 ctttgggcaa tagacagaac tcagactccg atggggaaaa gagttcttgc aaataccata
    13681 aaaaagcccc ttaaaaatct cgacatgata ctcctccgtc agcagtgcgt ttcaacattt
    13741 tactcagatg gaactcttct tagaaatatt cgggaagatc ttggaaaaat tagagatttt
    13801 gagcggacat taagccggat cctcgtcaag agaggaggtc ccagagagat aagaatgctc
    13861 gccgattcgc ttgtttttgc tatagggctc aaggattcga tcctgaaaag ggatgacctt
    13921 gaattcttta agtccactcc cgctttcaac atcaccggca aaattgcgca aaactggaaa
    13981 aaaagatttg tcgatgagcc cccgttcaat tataaagagg gtggttttgt ttcccctgac
    14041 cactctcctg aaatgttcca actatctgat cttattaata attccagagg acatattgcc
    14101 gctcttgagg caagggaaaa ggaatcgaca cagattccga cacttaaagc gggattcaac
    14161 cgcgttttcg gatattattt tgaggtgagc aaaagatttg ccgacaaagt tccggctcac
    14221 tacatcagaa aacagaccac tgcaaacggt gaaagatatt ttactgcaga acttaaggag
    14281 cttgaagaaa agatcctcac agccaaagat aaaatgactg tgcttgagct tgagatctta
    14341 gaaaaaacag tagtagaaat aaatggttat aaagaagaca tccttaatct tgcaaagtta
    14401 atttcttggc ttgacctttt ctgcggcttt gcaaagcttg ctgttgataa caattactgc
    14461 tgtcccgatg tcgttgaaaa ggagggcatt gaaattgttg acggaagaca tcctgtcgtt
    14521 gaagcttcaa tctctaaagg gagttatgtt cctgcaactg ttgttatcgg tgatgcacag
    14581 aaaagattct gcataatcac cggtcccaac atggggggaa aatcaacggt gatgaggatg
    14641 acagctctgg ttgttcttct tgcacagact ggctcttttg tccctgcaaa aagtgcccgc
    14701 atcgggatag ttgactccat tttcacccgg gtaggcgcaa gcgacaacct ttcaaaaggg
    14761 gagtccactt tccttgtcga aatgaaggaa accgcataca ttttaaattc ggcaacatct
    14821 aagagcttga taatacttga tgaaatagga agagggactg gaacttacga tggcatttca
    14881 cttgcccgtg caatagctga gcacattata aaaaaaataa atgccgttac cctttttgcc
    14941 actcattatc acatacttac agaacttgcc gaagattttt cctctgcttt caactttcat
    15001 atgggggcaa aggagtacgg tggaaagatc cgtttcacat atcagatgaa cgagggcggg
    15061 agttcaagaa gtttcggcgt tgaagttgca aaactgaccg acatgcccag aactgtaata
    15121 aaaactgccg aaaagatatt aaaggaaatg gagatggctg atagaaaatt ccgttttgaa
    15181 agaggcggct ctcttcagac cgatatcttt tcaatgaact tcgggcaggt cgaaacaatt
    15241 cccgatcatc tgcatgaact cgaaaacatg atcaggcgga tcgacccgga aaaaatgtcc
    15301 cctcgcgaag caatgaatat tttatttcaa ctatttgata ttgttaacga aaaaaaggag
    15361 aatgaaaatg gttaaagttg gcggaaacat caaggcgata accccttaca aaccgggaaa
    15421 accgatttct gaagttgaaa gagaactcgg gcttaaagac tccataaagc ttgcaagcaa
    15481 tgaaaatccc ctcggaccga gtgaaaaagt tgtcgaagcg gtcagagcag cggcatctga
    15541 acttaacttt tatcctgacg gaaatgcatt ctatcttaaa aatgcaattg tggaatatca
    15601 caaaaacaga ggtgaggaaa ttcattttga tgaactcatc gtgggcaacg gttccaacga
    15661 ggtaattgat atcgccataa gaacctttct ttcaggtaat gaagaaatta tcgtgtcaga
    15721 acaggcattt atcgtctatg aactcattcc tgccgcggca aacataaaaa cagttctgat
    15781 cccccagacg aaagatcatg ttgtcgatat tgatgcccac atcgccgcaa taaatgacaa
    15841 aacaaaaatg atctgccttg tcaatccgaa caacccgacc ggtacttatt attcaaaagc
    15901 cgcttttgaa aaacttctca aagcgatccc cgaagatgta atacttgcag ttgatgaagc
    15961 atat
//
LOCUS       DAOOYB010000039        15938 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_3605_length_15938_cov_13.121010, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000039 DAOOYB010000000
VERSION     DAOOYB010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 15938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15938
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3605_length_15938_cov_13.121010"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="PL056_03745"
     CDS             complement(<1..287)
                     /locus_tag="PL056_03745"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HPM46166.1"
                     /translation="MIAGVGTDIVEIERLKDKPDLWKRFLSLKDFEYIGKFKDPLERI
                     AGFWAAKEALVKALDDKTIEFTEINIIHNENGKPHFENVPHTGIFHLSISH"
     gene            complement(284..1624)
                     /gene="glmM"
                     /locus_tag="PL056_03750"
     CDS             complement(284..1624)
                     /gene="glmM"
                     /locus_tag="PL056_03750"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HPM46167.1"
                     /translation="MTEKKLFGTDGIRGKANVYPLLPETAVIVGKAITHYFSGNSSSR
                     ISAAIGMDPRISSPMLVNAVASGIMSAGGDVTYLGVVPTPLVSDFVRNNGCDFGIVIS
                     ASHNPYHDNGIKIFNSEGEKLNDEEELEIEQIFFDNPAGDGTVGKYAVKTDSYADYAN
                     RLSAVFSKNLNDCSFKICVDCANGAASFVAQQIFKNIEGITPHFFSKDPDGFNINDNC
                     GALHPEIMSKTVVEKQCDIGITYDGDADRLITIDELGNIVDGDKLIGIFARYLKNNGV
                     LKNNTVITTVMSNAGLEKFLKDEGIKLLRTNVGDRYVYEKIKETGSSLGGENSGHIIL
                     SDINPTGDGILASLMILAVMNATKKKLSELAADIELFPQFMTKVKVSEKKPLETIEGL
                     AELIEKEEKGLDGGRALVRYSGTEPVLRVMAEGPDMDKVKKAVENIAQFVRSTL"
     gene            complement(1621..2340)
                     /locus_tag="PL056_03755"
     CDS             complement(1621..2340)
                     /locus_tag="PL056_03755"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HPM46168.1"
                     /translation="MKEALKNFFTQYSSEKLLALLLTLILWTFSISRNQESRDYPVKV
                     NIIHPKDQIIVSDTIDQIQVKLSGSVFDFAKINDRDFVINIDISAKKPGKFTRLLDAK
                     MMTFAGNLSVEKIFPAEISVRTAKKIERKVKIDPWLDGQPPAGSKIKGYKVTPKKVTI
                     AGPEKEISEIDSVTTEKIVLDTLKGSFEKELKLVLPSIHISTVETDKATVTITLEKEA
                     KTIDADEPKSEDRVKAKKGEK"
     gene            complement(2337..3137)
                     /gene="cdaA"
                     /locus_tag="PL056_03760"
     CDS             complement(2337..3137)
                     /gene="cdaA"
                     /locus_tag="PL056_03760"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HPM46169.1"
                     /translation="MFLNYAGAYWQAIWGGSPSAMVIRVIDLMIVFFIIYRLLVLVKG
                     TKSMQILSGFLVLLFIYFIGNLVGLKTTEWILKGLFANFFIIIIILFHEEIRTILSGF
                     EFFDIFKGSGRKKDSLFVVDEITESLSYLASISEGALIVIAQKGNPESFVSGGVKIDS
                     NVKKELLLTIFKKSSPLHDGAVIIKDGRIALASAVLPLSTNPDIDPNFGTRHRAAYGI
                     SEAVDCIVFVVSEERGQISMLKKGKITRNMSRDIVRKVLIAHLVKEKK"
     gene            complement(3128..4324)
                     /gene="folP"
                     /locus_tag="PL056_03765"
     CDS             complement(3128..4324)
                     /gene="folP"
                     /locus_tag="PL056_03765"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HPM46170.1"
                     /translation="MLVFPFEVYSEEYDPLMKKLTVSSSGISIMKDRFKMKSFMISDI
                     STPAANVVKQHVLSLGGEAAVPAYSVNCSNPKADLIFSVREDRLPALLEKFRCQCWKL
                     PEVAEKIERLIINKPPWFSFSSEKFNTESPLIMGILNVTPDSFSDGGKFAKFENAVSH
                     AEKMILDGADIVDIGGESTRPGAATVEESDEIKRVVPVIKAIREKFPSTAISIDTTKS
                     RVAEAALDAGADIVNDISGLTFDTNMAVLAAERKAPVVIMHILGTPKTMQNNPVYDNF
                     FTETLEFLNNSIEIALSKGVRENSIILDPGICFGKDLNHNIAIIKHLEVYHSFAMPLL
                     AGVSRKSMIAKITGRDDPMERTAGTVSLNTKLLEKGVAVIRVHDIKEAKDCVAVHNAM
                     RDIKCF"
     gene            complement(4333..5352)
                     /locus_tag="PL056_03770"
     CDS             complement(4333..5352)
                     /locus_tag="PL056_03770"
                     /inference="COORDINATES: protein motif:HMM:NF017911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46171.1"
                     /translation="MKKFPPSIKDLVPLDNNGGIFFIHSVERYIIRKMKEELREYSAE
                     KNGVDLFFLDMSDGKNIIQEAVNTAREIGFFCSQKIVVLELAEKLSDKERDFLESYID
                     NCEPLNFLIVFVAEIDKRTKFFRSLQKMDKIYHPVPIPSTNDIRSFIISEFKPFTADE
                     ILISFFLNIENQDMFYIHSEIEKIKLYASVKGLKHISYEDSDEILNGLSEQVIFRIMD
                     LLSAGMVPQAMKLYRETLITEGDYKVNPLIISMFFKHFKALMKGRILLKENRANDFFS
                     YLTKSRLFYLKNNAVNTAGKYKNIKLLKALRKLSDIELGMKGAYGVRTTETTIEIEQF
                     MADYF"
     gene            complement(5355..7901)
                     /gene="leuS"
                     /locus_tag="PL056_03775"
     CDS             complement(5355..7901)
                     /gene="leuS"
                     /locus_tag="PL056_03775"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HPM46172.1"
                     /translation="MFYDHSVIEPAWQKKWRASDNFKYRGVQSGKPKYYMLEMFPYPS
                     GKLHMGHVRNYSIGDVVSRYYFRKGYDVLHPMGWDAFGLPAENAAIENGIPPAKWTYS
                     NIENMRERFFQLGFSYDWDREIATCSPDYYKWEQRFFIEMLSKGLVYKKNSEVNWCPQ
                     CNTVLANEQVEDGTCWRHGCEVEKKKIEQWYFKITDYAGELLDSLDDLPGWPDHVKEM
                     QRNWIGRSIGTYIDFQLEGTDKTVTVFTTRPDTLMGVTFASMAVEHEMAQFFIDNSKE
                     SEKMKNFIDEVKKLHLETRDDDYEKKGFFTGYNLIHPVDGRKVPLYFANFVLADYGTG
                     TVMAVPAHDQRDFEFAQKYGLALQIVIKPADSELTLPLEAAYTEAGIMVNSGIFDGMV
                     STEGKKAVNDHLVKTGKGKTGINYRLRDWGISRQRYWGTPIPVIYCDKCGTVPEKIEN
                     LPVKLPENVAFSGATSPLKDMPEFYETVCPECGGKARRETDTMDTFVESSWYHMKYCD
                     PKSVNEPFSKAEVDHAMPVDQYIGGVEHAVMHLLYTRFFTKVLADLGYVSFREPVKNL
                     LTQGMVCMETYHNGTGYLYPEEVYSKDGSHFDKSNDAPVKIGRVEKMSKSKKNVVDPV
                     RLIEKYGADTARLFVLFAAPPEKDLEWSEDGVEGCSRFLIRVWNIVNQNAELVKNCKK
                     IEKVDGEGKELWNKTHETIKKVTDEIQRFHFNTAISATMELLNSCYKFKPENEEQNNI
                     LGEALKDLIHLLNPFAPHITEELAHICGMEQLSESPFPEPLEAALVKDEILVIVQVNG
                     KLRDRITFSADVTKEDVEKTVRSKDYSKFLNSGEIRKIIYVPGKLINIVG"
     gene            complement(7921..10359)
                     /locus_tag="PL056_03780"
     CDS             complement(7921..10359)
                     /locus_tag="PL056_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46173.1"
                     /translation="MSRRLDTLKEQYISNPSLPSLIEYLEECKKEKLWETMIETVTGW
                     NGSETPEIHFYKGVALLNTGKREEGAEELRKVISHNPNHFAAKKEIENFGINDSQDAG
                     TVQGSNDALRKILVIEPRLESSEHYRNLKYKNMFLLIIITVAVLLFAYFMLRENRTDK
                     YNQMLEDPETSFTSLTYADFMSRIRELKIIDIRENIGDPLKKSILWILSYAVLDFHID
                     SQEELSQFKMFSTLVSAHEKPLEMITDYIESGAVPQGTELYHKLDADYPGSANEIKLL
                     KISDPGEITKKNLRESFYKALMLFRKKDFSAAAELNERVLAAFPGYELSQKLKVMIKA
                     GSSISGNIVLANISEELAVLEQWKTMSQERYFLGEAKVLLGRAADRDTVISDGFYSVC
                     PGRHFCTETVEYFISKGQTREASRMALYMKEQKENKRDADDIKLVVETSYADGDFSNC
                     YFSFRELTQFFGDSVDDATLMKGGECSERNGYFEEAVAIYEEINKKNQTIEITAKILR
                     MKYRLSREELYYTQMKDLAEKNPGSISVLYSYLDVLNRRNDIRETIKVLEKLYDLESS
                     DNRFRIIEEYIRQGAVFQAVKNLEKLKEDPAAAKTLSEIYNRYMLFDLADAVLPKDQT
                     IDPDWIFFREQLELFRNGEYEIVSKEVEKKMSSLEKCEPAFLHLKAEAFRNLGDKQRT
                     FGMIDNLLECNPYFMPGLMLAAEITYYQGDFTKAKDGIKYLLDNEEFLSPGKLYYHNY
                     LVLLNAEIMVAQGQENKLIPYLKKNLLAQMALGKKEFEKISDVSEKLKEQKQKELYEF
                     IKKFFRISVIATEK"
     gene            complement(10359..11255)
                     /locus_tag="PL056_03785"
     CDS             complement(10359..11255)
                     /locus_tag="PL056_03785"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPM46174.1"
                     /translation="MSELKRKIIEVLKGWEETLPEDVVPIVREFLDEVDENLVAEFLE
                     KYQTAGTDWGFSEGSKLVRLCLERVIEKMVPMDIRNGENLRTALAALKEGKVARIVIV
                     SNHLSYSDANIIAVAFSKYFKEYGFEDDLSVIAGPKVFSHPLRKFASMHFHSLLIAQS
                     QSVASLEVPYPIRVIARAAAKCAEDIKEKVKIFLVFPEGKRSRDGSLNQFLQGVYRLV
                     DTDENVLVLPVSVIGGDKFLPISCPNLHKTDAVISIGVAENIRDIARNIPFSSTVKQD
                     IMDYLGKKIAALHPEERRGVYR"
     gene            11486..12001
                     /locus_tag="PL056_03790"
     CDS             11486..12001
                     /locus_tag="PL056_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46175.1"
                     /translation="MKPQDVLVLFKLLIFGGRFVPYSEFAKEIFLSTSELHSSIKRSE
                     KNGLFSADTNRVIISALKEFVFHGAKYVFPVEIGMVTRGIPTSFAAEPLKSELSQSDI
                     EIPVWPDAEGTHRGISFSPIYKTVPDAAKKDEKLYRLLALFDALRSGRARERAVAKEL
                     MEREIELYAKD"
     gene            11988..12686
                     /locus_tag="PL056_03795"
     CDS             11988..12686
                     /locus_tag="PL056_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46176.1"
                     /translation="MPKINADDMLRVHKSLKSAGLDFVFIGGGILGFLIDDPLATPVR
                     TTIDIDVVLDIVTDPGQIMLEEKLGSAGFKHDMSDRAPRCRWIINNIKVDILSLKDRF
                     SGMNLKWLQETVSMPLEIKEEETVFKIASPACFIAMKLEAFKDRGNADYLGSRDIEDV
                     ISVIDGRSSIVEEIKSSDPKVVEFISKTIAELLWKNEFTASISGHLMPDPASQKRRLI
                     VIERLNRISQFQGD"
     gene            12691..13878
                     /locus_tag="PL056_03800"
     CDS             12691..13878
                     /locus_tag="PL056_03800"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HPM46177.1"
                     /translation="MKYEKSVILEKGRERLARNHNPWIYSGAVRSINGNPQDGDIVAV
                     LDFEKTFVAWAHYNSRSGIALRLLEWNEKVDPDILWIRKKISEAYELRKKMIGSDTNL
                     FRLIYSESDMLPGIICDIYDSVAVLQISTPGFDRLKKDIAEIISEIVGISSVYEKSDG
                     DGRRIEGLAPSAGWIKGESDSTELIVKENGLNFFIDLSGQKTGFYADQRENRLLFGKI
                     ATGEVLDMCSFSGAFSIHALKNGASHVTLCDISKETLKLIDKNMEINDIAKEKHDFIA
                     GDAFEVLRNLERNGRKFDSIVLDPPKLVPSNKYLEKGLKAYKDLNFNALKLLRDGGVL
                     ATFSCSGAVTMADFKNAVAYAAKDTGKEIKILHQMHQPFDHPVRMSAPETEYLKGLLL
                     KVC"
     gene            13879..14526
                     /locus_tag="PL056_03805"
     CDS             13879..14526
                     /locus_tag="PL056_03805"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HPM46178.1"
                     /translation="MKTDSYQKKIIAIAIILTVAVTAFALWMNTTKFEFRYYVFPAVL
                     IFLPLVFNSSFRPQNILKPSKKGFKLFFYVTITVIVLYPPAFFIYWITLKNQVFMIPG
                     TEEVLKAVLKGLTAVAVAAIPEEFFFRGYLQEHVFRGLERKFIKVVTLKNLITSILFG
                     VVHAVAFLDITRAATFFPSLLFGLFCEKSEGKIFYSILFHVISNILAFVLWTFIK"
     gene            14627..>15938
                     /locus_tag="PL056_03810"
     CDS             14627..>15938
                     /locus_tag="PL056_03810"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPM46179.1"
                     /translation="MERAVMNSLLEWREKKNRKPLIIQGARQVGKTHLLKEFGKRHFE
                     KYHYINFENKSVLSEVFEKTLEPASIIMQIEFYLETKIDKDKDIIIFDEIQREPRALT
                     SLKYFCEEMPEIAVCAAGSLLGVTMNIDSFPVGKVDMLELRPLNFSEFLIATEDKMVS
                     DFLNGYDLTKPLPVIIHQKLMEIWKHYLIVGGLPEAVEHYRTHRDDLFSAFSKVREIQ
                     EVLLKTYMADIAKHSGKINSIHIEQVFMSVPAQLAKSVDESAKRFIFKNVVKGINGYN
                     RLSNPIGWLERADLILRVPVIEHVEIPLIAQVKPNIFKLYFFDVGLLGAISELTPLQI
                     LRFDFTTYKGFFTENFVLQELISSGITRTYCWQGRTSEIEFLLQTNAGDIVPVEVKSG
                     AVTQSKSLSVYNERYKPSFSIVVSAKNEGRQPKRWYVPVYAAGKL"
BASE COUNT         4398 a         3388 c         3117 g         5035 t
ORIGIN      
        1 tcatggctga tggaaagatg gaagattccg gtgtgaggga cattttcaaa atggggtttg
       61 ccgttttcgt tatgaatgat attaatctca gtaaattcaa tggttttgtc atcaagtgcc
      121 ttgacgagtg cttcttttgc cgcccagaag ccggctatcc tttcaagcgg atctttgaat
      181 tttccaatgt attcaaaatc tttaagggat aaaaaccttt tccatagatc gggcttatct
      241 ttaagtcgct caatttcaac aatatcagtt ccaactcctg cgatcaaagt gtgctcctga
      301 caaattgtgc aatgttttca actgcttttt taactttgtc catgtcgggg ccttccgcca
      361 tgacacgaag aacgggctca gttccgctgt acctgacaag tgctctgccg ccgtcaagtc
      421 ccttttcctc tttttcaata agttcggcaa gcccttcaat tgtttcaagc ggctttttct
      481 cactcacttt cacttttgtc ataaactggg gaaaaagctc aatatctgct gcaagttctg
      541 ataacttttt ctttgttgcg ttcataactg caaggatcat gagcgaagca agaattccat
      601 ctcctgtcgg attaatgtca gaaagaataa tgtgtccact gttctctccg ccaagagatg
      661 agccggtttc cttaattttc tcgtaaacat atctgtcgcc gacatttgtt cttagaagtt
      721 taatcccttc atcttttaaa aacttttcaa gccctgcgtt gctcataacc gtcgtaataa
      781 cggtgttatt tttcaacacc ccgttatttt tgaggtatct ggcaaaaatg ccgatcagtt
      841 tgtctccgtc aacaatattt ccaagttcat caattgtaat aagccggtcg gcatcaccgt
      901 cataagttat cccgatgtcg cattgttttt ccaccacagt ttttgacatt atttcaggat
      961 gaagcgcccc gcagttgtca ttgatgttga aaccatccgg atcttttgag aaaaaatgcg
     1021 gtgtaattcc ttcaatgttt ttaaatattt gctgcgccac aaaagatgcc gcgccatttg
     1081 cacagtcaac gcatatttta aaagaacagt catttaaatt tttggaaaaa acggcactga
     1141 gccggtttgc ataatcagca taagagtcag ttttcacagc atatttgccg acagttccgt
     1201 cacctgccgg gttatcaaag aatatttgtt cgatctctaa ctcctcttca tcattgagct
     1261 tttcaccttc tgaattaaat atttttattc cgttatcgtg ataaggattg tgacttgcag
     1321 agatcactat gccgaaatca cagccgttgt tccttacaaa atcgctcaca agaggtgtcg
     1381 gaacaactcc aagataagtt acatcgcctc cggcactcat aatgcccgat gctacagcat
     1441 ttaccagcat tggcgaggaa attctggggt ccatacctat tgcggcactg atccttgaac
     1501 tggaatttcc tgaaaaataa tgggttatgg cttttccaac aattacggct gtttcaggta
     1561 aaagtggata aacattggct tttcccctta tcccgtcagt cccgaataat ttcttttctg
     1621 tcatttttca cctttttttg cttttaccct gtcttcgctc ttcggctcgt ccgcatcaat
     1681 tgttttcgcc tctttttcaa gtgttatcgt aacagtcgcc ttgtcagttt caacagttga
     1741 gatatgtatc gagggaagaa caagtttcaa ttccttttca aaagacccct tcagagtatc
     1801 gagtactatc ttttcggtag ttaccgagtc aatctccgag atctcctttt ctggaccggc
     1861 aatagtaact tttttcggag tgaccttata tccttttatc tttgacccgg cgggtggctg
     1921 gccgtcaagc cacggatcaa tttttacttt tctttcaatt tttttagccg tccttaccga
     1981 aatctctgcc ggaaatattt tttcaacact cagattgccg gcgaaagtca tcatttttgc
     2041 atcgagaaga cgggtgaact ttcccggttt cttcgcagaa atatcaatgt ttataacaaa
     2101 atccctgtcg ttgatctttg caaaatcgaa aacgcttcct gaaagtttta cctgtatctg
     2161 gtcaatggta tcagaaacga ttatctgatc tttggggtgt atgatattaa ctttgaccgg
     2221 ataatctctt gattcctgat tcctgctgat cgaaaatgtc cagaggatca gagtcagcag
     2281 cagtgcaaga agtttttctg aagaatactg tgtaaaaaaa ttctttagtg cttctttcat
     2341 tttttctcct tcacaaggtg ggcaatgagc acttttctga caatatcacg gctcatgtta
     2401 cgagttatct tccctttttt gagcatggat atctgccctc tctcctcgct cacgacaaaa
     2461 acaatacagt caaccgcttc acttattccg tatgcagcac ggtgccgtgt cccgaaattg
     2521 gggtcaatat cgggatttgt actcagcggg agaacggcac ttgcaagagc gatccttccg
     2581 tcttttatta tcacggctcc gtcatgaaga ggtgagcttt ttttgaatat tgtaagaaga
     2641 agttcctttt ttacatttga atcgatcttt accccccccg aaacaaacga ttcgggattt
     2701 cctttctgag caatgactat cagcgctccc tcagagatag atgcaagata tgataatgat
     2761 tcagttattt catcaacaac gaataagctg tccttctttc ttcccgaacc tttaaaaatg
     2821 tcgaagaact caaatcctga aagaatcgtc ctgatctctt catgaaaaag aatgatgatt
     2881 atgatgaaaa agttggcaaa aagacctttg agtatccatt cggttgtctt aagtccgacc
     2941 agattcccga taaagtaaat gaaaagaaga acgagaaacc ccgacagtat ctgcatcgac
     3001 tttgtccctt ttaccagaac aagcagacgg tagataatga aaaagacgat cataaggtca
     3061 atgacccgta tcaccattgc cgacggcgaa ccgccccaga tcgcctgcca gtatgcccct
     3121 gcatagttta aaaacatttt atatccctca ttgcattatg aaccgccaca cagtctttcg
     3181 cttcctttat atcgtgaacc cttattaccg caaccccttt ttcaagcagc tttgtattga
     3241 gtgacaccgt tcccgctgtt ctttccatcg gatcgtctct gccggttatc ttcgctatca
     3301 tgctttttct gctcactccg gcaaggagag gcatggcaaa tgaatgatag acctcaagat
     3361 gttttataat tgcgatattg tggttgagat ctttgccgaa acagatgccc gggtcaagta
     3421 taatgctgtt ttctctgaca ccttttgata gagcaatctc tatggaattg ttgagaaatt
     3481 ccagtgtctc agtaaaaaaa ttgtcgtaaa caggattgtt ctgcatggtt ttgggagttc
     3541 ccaatatatg cattatcaca acaggtgcct ttctttcggc agcaagaact gccatgtttg
     3601 tatcaaaagt cagaccactg atatcattaa caatatcagc tccggcatca agagctgctt
     3661 ccgcaactct gctttttgtg gtatcgatcg atattgcagt tgacgggaat ttttccctga
     3721 tcgctttaat aacagggaca actctcttta tttcatcgct ttcttcaaca gttgccgctc
     3781 caggacgggt tgattctccg ccgatatcga cgatgtccgc cccgtcaaga atcatttttt
     3841 ctgcatgaga aaccgcattt tcaaattttg caaacttccc tccgtcagag aacgagtcag
     3901 gagtaacatt aagaattccc attatcaacg gactttctgt gttaaatttc tctgatgaaa
     3961 atgaaaacca cggaggttta tttatgatca atctctcaat tttttcagca acttcaggaa
     4021 gtttccagca ctgacatctg aatttttcaa gcagagccgg cagccggtct tcccttactg
     4081 aaaagatcaa gtcggccttc ggatttgaac aattcacaga atatgcagga actgccgctt
     4141 caccgccaag agaaagaaca tgctgtttca ctacattggc tgcaggagtg gaaatatctg
     4201 atatcataaa gcttttcatt ttaaaacggt ctttcattat tgatatgccg ctacttgaga
     4261 cagtcagttt tttcatcaac gggtcatatt cttcgctgta aacttcaaat ggaaaaacta
     4321 acaagtccgc tcctaaaaat aatccgccat gaactgttcg atctcgatcg ttgtttcagt
     4381 cgttctcact ccgtatgcac ccttcatgcc cagttcgatg tcagagagtt ttcttaatgc
     4441 tttcaacaac ttaatgttct tatattttcc tgcggtgttc accgcattgt tcttgagata
     4501 gaaaagtcta ctttttgtca ggtatgaaaa aaaatcgttt gctctgttct ctttaagcag
     4561 tatccttccc ttcatcagcg ccttgaaatg tttaaaaaac attgaaatta ttaaaggatt
     4621 cactttatag tcaccttctg taataagggt ttccctgtaa agtttcatcg cctgcggaac
     4681 catcccggct gaaagaaggt ccatgatacg gaatatcacc tgttcgctca acccgttcag
     4741 aatttcgtca gaatcttcat aacttatatg cttaagaccc ttaaccgatg cataaagctt
     4801 tatcttctct atctctgaat gaatgtagaa catgtcctga ttttcaatgt tgagaaagaa
     4861 cgatatgagt atttcatcag cggtgaaagg tttgaattca gatattataa aactcctgat
     4921 atcgttagtt gaagggatgg gcacaggatg atatatttta tccattttct gtaatgatct
     4981 gaaaaactta gtccttttat caatctctgc tacaaagacg atgagaaagt taaggggttc
     5041 gcagttgtct atataagatt caagaaagtc gcgctctttg tcgctcagtt tttctgcaag
     5101 ttcaaggacc acgatcttct gtgaacagaa aaatccgatc tcgcgtgctg tattgaccgc
     5161 ctcctgaata atgttctttc catcgctcat atcgagaaaa aaaagatcca cgccattctt
     5221 ttctgctgaa tattcgcgca gttcctcttt cattttcctg ataatatatc tttcgacaga
     5281 gtgaatgaaa aatattccgc cgttattatc aagcggaaca agatctttta ttgaaggggg
     5341 aaattttttc ataattatcc gacgatgttt atcagttttc cggggacata aattattttc
     5401 cttatttcgc cactatttaa aaattttgag taatctttgg accttactgt tttttcaaca
     5461 tcttcttttg tcacatctgc actgaatgtt atcctgtccc tgagctttcc gttgacctga
     5521 actatcacga gaatctcgtc tttaacaagt gccgcttcaa gtggttcagg aaaaggagat
     5581 tctgaaagtt gctccattcc gcagatatga gcgagctctt cagtgatgtg gggagcaaac
     5641 ggatttaaaa gatgtatcag atctttcaat gcttcgccga gaatattgtt ctgctcttca
     5701 ttttccggct tgaacttata acaactattc agaagttcca ttgttgcgct tattgccgta
     5761 ttaaagtgaa agcgctggat ctcatcagtc acttttttga ttgtttcgtg tgttttgttc
     5821 cagagctctt ttccttcacc atcaactttt tctatctttt tacagttctt aacaagttca
     5881 gcgttctggt ttacgatatt ccagaccctg ataagaaaac ggctgcaccc ttcaactccg
     5941 tcctcggacc attcaaggtc tttctcaggc ggagcggcaa aaagtacgaa caggcgggca
     6001 gtatcggcac cgtatttttc gatgagtcgc acaggatcga caacattttt ctttgatttg
     6061 ctcatttttt caaccctgcc gatcttgaca ggcgcatcat tgcttttatc aaaatgcgat
     6121 ccgtcctttg aatacacttc ttcagggtat aagtaaccgg ttccattgtg ataagtctcc
     6181 atgcagacca ttccctgggt cagaaggttc tttacgggtt ccctgaaact gacatacccc
     6241 agatcggcca gcactttcgt gaaaaaacgg gtgtagagaa ggtgcattac agcgtgttca
     6301 actccgccga tatactgatc aaccggcatt gcatggtcaa cttcggcttt agaaaatggt
     6361 tcattcacgc ttttagggtc acagtatttc atgtgatacc aggagctttc aacaaatgtg
     6421 tccattgtgt cggtctctct tcttgccttt ccgccacatt caggacagac agtttcataa
     6481 aattcaggca tatccttcaa agggcttgtt gcacctgaaa acgcaacatt ttcaggcagt
     6541 tttacaggaa gattctcgat cttttcagga acagtcccgc atttgtcgca gtatatgaca
     6601 ggaatagggg tgccccagta tctctgacga cttatacccc aatcgcggag acggtaattg
     6661 atgcctgttt taccttttcc ggtcttgaca agatggtcgt ttaccgcttt tttcccttcc
     6721 gtcgaaacca ttccgtcaaa aattccgctg ttcaccatta ttcctgcttc ggtatatgcc
     6781 gcttcaagcg gaagtgtgag ctcactgtcc gcaggtttta taacgatctg taaagccaga
     6841 ccgtactttt gagcaaattc aaaatccctc tgatcgtgag cagggaccgc cattaccgtc
     6901 cctgtgccgt aatctgcgag aacgaaattt gcaaaataga gaggaacctt ccttccgtca
     6961 accgggtgga tcaggttgta tccagtgaaa aaccctttct tttcgtaatc gtcatccctt
     7021 gtttcaagat gcagtttttt cacttcgtca atgaaattct tcatcttttc gctttctttg
     7081 ctgttgtcaa tgaagaactg cgccatttca tgttcaacag ccatgctggc aaaagtcact
     7141 cccatcaaag tatcgggcct tgtggtgaaa acagtgaccg ttttatctgt cccttcaagt
     7201 tgaaaatcga tatatgtgcc gatacttctc cctatccagt ttctctgcat ctcctttaca
     7261 tgatcgggcc atcccggaag atcgtcaagt gagtcaagaa gttctccggc ataatcagtg
     7321 atcttgaaat accactgctc gatctttttc ttttcaactt cacagccgtg ccgccagcag
     7381 gttccgtctt ccacctgttc atttgcaaga actgtgttgc actgcggaca ccagttaact
     7441 tcgctgttct ttttgtaaac cagtcctttg ctgagcattt cgataaaaaa gcgctgttcc
     7501 catttataat agtcaggcga acatgttgcg atctctctgt cccagtcata ggaaaaaccc
     7561 agctgaaaaa atctttccct catgttttca atgttgctgt atgtccattt tgcaggaggt
     7621 attccgttct ctattgcggc attttcagcc ggaagtccga aagcatccca tcccatcgga
     7681 tgaagcacat catacccttt tctgaagtaa tatcttgaaa ccacatctcc gatcgaatag
     7741 ttccttacat gccccatatg aagttttcct gatggatagg gaaacatttc aagcatgtaa
     7801 tatttcggct tcccggactg aactcccctg tatttaaagt tgtcggaagc tctccatttc
     7861 ttctgccaag ctggttcaat tacagaatga tcgtaaaaca tttttcctcc ggaatgagat
     7921 tcatttttct gtggcaatca cggaaattct aaaaaatttc tttataaatt catatagttc
     7981 cttctgtttc tgttctttga gcttttcaga gacatcgctt atcttttcaa attctttctt
     8041 ccccaaagcc atttgtgcaa gaagattctt tttcagatac ggaataagct tgttctcctg
     8101 tccctgagca accattatct cagcattgag aagaacaaga taattgtggt aatagagttt
     8161 gcctggagag aggaactcct cattatccag aagatatttt ataccgtctt tggctttggt
     8221 gaaatcaccc tgataatatg ttatttctgc ggccagcata agtcccggca taaaataagg
     8281 attgcattca agaagattgt ctatcattcc gaaagttctc tgtttgtccc ctaaattcct
     8341 gaaagcttcg gctttaaggt gaagaaaagc cggctcacat ttttcaagag aagacatttt
     8401 cttctcaact tcctttgaga ctatctcata ttctccgttc ctgaacagtt ccaactgttc
     8461 tctgaagaat atccagtcag gatcaattgt ctgatctttc ggaagcactg catcggcaag
     8521 atcaaaaagc atgtatctgt tatatatttc tgaaagtgtt ttggcggcgg cagggtcttc
     8581 tttcagtttt tcaagatttt tcaccgcctg aaaaaccgct ccctgcctga tgtattcctc
     8641 gatgatcctg aacctgttat cagaagattc aaggtcataa agtttttcaa gcacttttat
     8701 ggtttccctg atgtcgttcc ttctgttgag aacatcaaga tatgaataga gaacagaaat
     8761 gctcccggga tttttttccg cgaggtcctt catctgagta tagtaaagct cctctctgga
     8821 aagccggtat ttcatcctca gtatttttgc agtgatctct atcgtctggt tctttttgtt
     8881 tatctcctcg taaattgcaa cggcctcttc aaaatagcca ttcctttcgc tgcactcacc
     8941 tcccttcatc agggttgcat catccacgct gtccccgaaa aactgagtaa gctccctgaa
     9001 tgaaaaatag cagttgctga aatctccgtc agcataactt gtttcgacaa caagctttat
     9061 atcatcagcg tctcttttgt tctctttctg ctctttcatg taaagagcca ttcttgatgc
     9121 ttcccttgtc tgacctttgc tgatgaaata ttcaacggtc tctgtgcaga aatgccttcc
     9181 cgggcataca gaataaaatc cgtcggagat aacggtgtct ctatccgccg ctcttccgag
     9241 aagaaccttt gcttctccca gaaaatatcg ctcctgactc atagttttcc actgttcaag
     9301 cacagcaagt tcttctgaaa tatttgcaag aactatattt cctgatattg atgaaccggc
     9361 ttttatcata actttcaatt tctgtgacag ctcatagccg ggaaatgccg caagcaccct
     9421 ttcgttaagt tctgcggccg cactaaaatc cttttttctg aaaagcatga gagctttata
     9481 gaaagattct ctcaagttct ttttcgtgat ctcacccgga tcacttattt tcagcagttt
     9541 gatctcgttt gcagagccgg gataatccgc atcaagctta tgataaagct ccgtaccttg
     9601 aggaacggca cccgattcaa tatagtctgt gatcatttca agcggttttt catgagccga
     9661 aacgagcgtt gagaacattt taaactgaga cagctcctcc tgtgaatcaa tatgaaaatc
     9721 aaggacggca tatgaaagta tccacagaat gctctttttg agcggatcac cgatgttctc
     9781 gcggatatca atgatcttaa gttcccttat cctgctcata aaatcggcat atgtcaaaga
     9841 ggtgaagctg gtttcaggat cttcaagcat ctggttgtat ttgtcggttc tgttctccct
     9901 gagcataaag tatgcaaaaa gcagaacggc aaccgtgata atgatcagaa ggaacatgtt
     9961 cttatatttg agattccggt agtgctcact gctttcaaga cgcggctcta ttacaaggat
    10021 cttcctgagt gcatcatttg atccctgcac ggttcctgca tcctgagaat catttatccc
    10081 gaaattctcg atctcttttt ttgcagcgaa atggttcgga ttgtgactga tcacttttct
    10141 gagctcttct gccccttcct ctctttttcc ggtattgagg agcgccaccc ctttgtagaa
    10201 atggatctcc ggagtttcgc ttccgttcca gccggtcact gtttctatca tcgtctccca
    10261 gagcttttcc tttttacatt cctcaagata ttcgatgaga gaagggagtg agggatttga
    10321 aatgtactgt tctttcagtg tatcaagccg tctgctcatc agcggtatac cccccttctt
    10381 tcttctgggt gaagagctgc gatcttttta cccagataat ccataatatc ctgtttgaca
    10441 gtggatgaga aaggaatgtt tcgtgcaata tctctgatgt tttcagccac tccgatgctt
    10501 attacggcat ctgttttatg aagattcggg caggatatcg gaagaaattt gtctccgccg
    10561 ataacagaaa caggaagaac aaggacattt tcgtctgtat caacaagccg gtaaactccc
    10621 tgaagaaatt gattcagaga cccgtctctg cttcttttac cttcaggaaa aacaaggaat
    10681 atcttaactt tttccttgat atcttctgca cattttgcgg cggctcttgc tatcaccctt
    10741 atcggataag gaacttctag agaggcaaca gactgtgact gggcaattaa aagtgaatga
    10801 aaatgcatgc ttgcaaattt tctcaacgga tgggaaaata ctttagggcc tgcaattaca
    10861 gaaagatcat cttcaaaacc gtactctttg aaatatttac tgaaagctac tgcgatgata
    10921 tttgcatctg aataactcaa atgatttgaa acaattacaa ttcgtgcaac tttgccttcc
    10981 ttgagggccg cgagggcagt tcttaaattt tctccattcc tgatatccat gggaaccatt
    11041 ttttcaataa ctctttcaag acagagccgc accagttttg acccttccga aaacccccag
    11101 tcagttccgg cagtttgata tttttcaaga aattcggcaa ctaagttttc gtcaacttca
    11161 tcaagaaatt ctctgacaat gggaactaca tcttcaggaa gtgtctcctc ccaacctttc
    11221 agaacttcga ttatttttct tttcagctca gacattatcc ggttcttctc ctataataat
    11281 taacaaagag agtatttctt ataccagtat cactaaaaaa atcaagataa caccgcttat
    11341 ataaaaatct tcaacatcta acattaagtg aattgatata gctgtgggaa taaattgaaa
    11401 agctaagttt tttgttgaca atatgattta taagattacg atatttttag atattcgctg
    11461 ttcgcgaata gcgtatggag gtaaattgaa gccacaggac gttctggttt tattcaaatt
    11521 gctgatcttt ggcgggagat tcgtccctta ctctgaattt gcaaaagaga tttttttatc
    11581 aacttcagaa cttcactctt caattaaaag atctgaaaaa aacggtcttt tttcggctga
    11641 taccaacagg gtgataatat ctgctttaaa agagtttgta tttcacggag cgaagtatgt
    11701 ttttcctgtt gaaattggta tggtaactcg tggaattcca acgagttttg ccgcagaacc
    11761 gctgaaaagc gaactttctc aaagcgatat tgaaataccg gtctggcccg atgctgaggg
    11821 aactcaccgc ggcatttctt tttcaccgat atataaaact gttcctgacg ctgcaaaaaa
    11881 agacgaaaaa ttataccggc ttcttgctct ttttgatgca ttgagaagtg gcagagcaag
    11941 agaaagagct gttgcaaaag aattgatgga acgggagatt gagctgtatg ccaaagatta
    12001 atgcagatga tatgttaaga gtccataaaa gtttgaagtc ggctggtttg gactttgttt
    12061 tcatcggtgg cggcatactt ggttttttaa ttgatgaccc cctggcaact ccggtaagaa
    12121 caactattga cattgatgtt gtgcttgaca ttgtcaccga tcccgggcag atcatgcttg
    12181 aggaaaaact cggttcagcc ggtttcaagc acgatatgag cgatagagct ccccgttgtc
    12241 gctggataat taacaacatt aaagttgata ttctttcgct taaagacaga ttttcaggaa
    12301 tgaatttgaa gtggcttcag gaaacggttt caatgcctct ggaaataaaa gaagaagaaa
    12361 ccgtttttaa aattgcatcc ccggcatgtt ttattgctat gaaacttgaa gctttcaaag
    12421 ataggggaaa tgccgattac ctgggcagca gggatataga agatgtgata tctgtcattg
    12481 atggaagatc ttcaattgtt gaagaaatta aaagttctga tccaaaagtt gttgagttta
    12541 tttcaaaaac aatagcagag ttgctttgga aaaatgaatt tacagcttca atatcaggtc
    12601 accttatgcc tgatccggca agtcagaaaa gaagattaat tgttattgag cggcttaaca
    12661 ggatcagtca attccaggga gattaaactt atgaaatatg aaaaatcggt tattcttgaa
    12721 aaagggcgtg aaagattggc aagaaaccat aatccgtgga tatattccgg agcggtgaga
    12781 tctatcaacg gcaatccgca agatggtgat attgttgcag ttcttgattt tgaaaaaaca
    12841 tttgtggcat gggcacacta caacagcaga tcgggaatag ccctgcggct tcttgagtgg
    12901 aatgaaaaag ttgatcccga tatactatgg attagaaaaa aaatatctga agcttatgaa
    12961 cttcgtaaaa aaatgatcgg ttctgataca aatctgttcc ggcttatcta ttctgagtcc
    13021 gatatgcttc cggggatcat ctgcgatatt tacgattcgg tcgccgttct tcagatatca
    13081 actccgggat ttgacagatt aaagaaggat attgctgaaa taatatcaga aattgtagga
    13141 atttcatctg tttatgaaaa aagtgacggg gatggcagga ggatagaagg tcttgcccct
    13201 tcagccggat ggataaaagg agaatctgat tcaacggaac tgattgttaa agagaacggt
    13261 ctgaattttt ttattgatct gagcggacag aagactggtt tttatgccga ccagagagaa
    13321 aaccgccttc tttttggaaa aattgcaaca ggcgaagtgc ttgacatgtg ctcgttttca
    13381 ggtgcttttt caatacacgc tctaaaaaac ggagcgagcc atgtaacttt gtgcgacatt
    13441 tcaaaagaaa ccttgaagct tatcgacaag aatatggaaa ttaatgatat tgctaaagaa
    13501 aaacatgatt ttattgcagg tgatgcgttt gaagttttaa gaaatctcga aaggaatgga
    13561 agaaaatttg attccattgt ccttgatccg cctaaacttg tcccttcaaa taaatatctt
    13621 gaaaaagggc tgaaagctta caaagatctc aatttcaatg ctctgaaact tctgcgggac
    13681 ggaggtgttc ttgccacttt ttcctgttcg ggagcggtaa caatggctga tttcaaaaac
    13741 gctgtcgcct atgcggcaaa agacacagga aaagagataa aaattcttca tcagatgcat
    13801 cagccatttg accatccggt gaggatgagt gcccctgaaa ctgaatatct caaaggcctt
    13861 cttttaaagg tttgttaaat gaaaacagat agttatcaaa agaaaatcat agcgatcgca
    13921 attatactga ctgttgcggt gacggctttt gcactttgga tgaacaccac aaaatttgag
    13981 ttcagatact atgtttttcc cgctgtgctg atatttctcc ctctcgtttt taacagctct
    14041 ttccgtcctc aaaacatctt gaagccgtca aaaaaagggt tcaaactctt tttttatgtc
    14101 actataaccg ttattgttct ttatccgcct gcatttttca tctactggat aacgcttaaa
    14161 aatcaggtat ttatgattcc agggacggaa gaagttctca aagcggtctt aaaaggactc
    14221 actgcggtcg cagttgccgc gatccctgag gaattctttt tcaggggcta tcttcaggaa
    14281 catgttttca gagggcttga aagaaagttc ataaaagtcg ttacactgaa aaaccttata
    14341 acttcaatac ttttcggcgt tgtgcacgca gttgcttttc tagacataac aagagccgca
    14401 acattctttc catctcttct tttcggtctt ttctgtgaaa aaagtgaagg gaaaatattt
    14461 tacagcatcc tttttcatgt gatctcaaac attcttgcgt tcgttctgtg gacttttatt
    14521 aaataaagtc cttgcacttt ttatacaaat atgacaaaaa gtccttgcac tttttataca
    14581 aatatgttaa ttttagcaag cacttttgtt tttgagaggt gaaaaaatgg aacgagctgt
    14641 tatgaattct cttcttgagt ggagagagaa aaagaataga aagccactta ttattcaagg
    14701 agcaaggcag gttgggaaaa cccatctgct caaagaattt ggtaaaagac actttgaaaa
    14761 gtatcattat attaattttg aaaacaaaag cgttttgtcg gaagtgtttg aaaagacgct
    14821 tgagccggca tcaataataa tgcagataga gttttatctc gaaacaaaaa ttgataaaga
    14881 taaagacatt ataatttttg atgaaatcca aagagagccg agagctttga catcgctaaa
    14941 atatttttgt gaagagatgc cggaaattgc tgtgtgtgcc gcaggttcgc ttcttggtgt
    15001 gacgatgaat attgattcat ttccagtcgg caaagttgat atgcttgagc tgagaccgct
    15061 caacttttca gagtttttaa ttgcaactga agacaaaatg gtatcagatt ttttaaatgg
    15121 atatgacctg acaaaaccgc ttcctgtgat aattcatcag aaactaatgg agatatggaa
    15181 gcattatctc attgtgggag gtctgcctga agcagttgag cattacagga ctcaccggga
    15241 cgatcttttc agtgcttttt caaaggttag agaaattcag gaagtgcttt taaaaacata
    15301 tatggccgat attgccaaac acagcggcaa gataaattct atccatattg aacaggtttt
    15361 catgtctgtt cctgcccagc ttgcaaaaag tgttgatgaa tcggcaaaaa gattcatttt
    15421 taaaaatgtt gtgaagggaa taaacggata taaccggctt tcaaatccga tcggctggct
    15481 tgagagggcg gatcttattt tgagagtgcc cgttatcgaa catgttgaaa ttcctttaat
    15541 tgcacaggtt aaaccgaata ttttcaagct ttatttcttt gatgtcggac tgcttggagc
    15601 aataagtgaa cttacacctt tgcagatact tcggtttgat tttacaacat ataaaggttt
    15661 tttcacagag aactttgttt tgcaggaatt aatatcatca ggaattacaa gaacttactg
    15721 ttggcagggg agaacatctg aaatagagtt ccttcttcag acaaatgcag gggatatcgt
    15781 tccggttgaa gtcaaatccg gcgcagtgac acaatcaaaa tcactctcag tttataatga
    15841 acggtataaa ccgtcttttt caatagtcgt cagtgcaaaa aatgagggca gacagccgaa
    15901 aagatggtat gtcccagtct atgccgcagg gaaacttt
//
LOCUS       DAOOYB010000040        15938 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_3606_length_15938_cov_11.115910, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000040 DAOOYB010000000
VERSION     DAOOYB010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 15938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15938
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3606_length_15938_cov_11.115910"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            12..854
                     /locus_tag="PL056_03815"
     CDS             12..854
                     /locus_tag="PL056_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46180.1"
                     /translation="MRILIFSILLIPGLLFADKIVVWNLKPQAGVTEKEAATVSSIVT
                     TEIARVSKSTIISESEMKSVIDAEQMKMSCGAEDSSCIAEIGAAMGAPLSVSGTFSKM
                     GDYWIITLQLVDVRKVEVQSRVSKKFKGNENALVEAITPMICELFNDKECMEKSVQQT
                     KEDQKNEKKEKTPVSWKKVTGWVLTVPGVLMFGSGVAAHILMKNAKEDYELTGVDEGR
                     YKSFRTYSTVGYVTGSVLMAGGIALLIWDGVEKSKKNKKNEAVSFYVIPSFDGGYAGM
                     QWSW"
     gene            854..>1056
                     /locus_tag="PL056_03820"
     CDS             854..>1056
                     /locus_tag="PL056_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46181.1"
                     /translation="MKKIFFAVTVFLMFSGCSDFKDSLIGTVGEKCFGNGTCKDGLIC
                     IEGKCAKDEEAENDDDSGNTGDTG"
     assembly_gap    1057..1156
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1157..2595
                     /locus_tag="PL056_03825"
     CDS             <1157..2595
                     /locus_tag="PL056_03825"
                     /inference="COORDINATES: protein motif:HMM:NF012632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46182.1"
                     /translation="TGDDDDVSNVETEVDDDVINYPCDPNPCATLLNSTGECSPDVDD
                     SHVCTCNDGFEWVFEDKVCNAIPDPCLNDPCTTLADSTCVPEKTEDQQNFTGMLECVC
                     NEGFDKSFIGSEICVPAFKSVSTGKDHACAVDYYDKLYCWGSNFNAQLGNSLTGTGEG
                     CLAEHPFKVDDNSWTAVSAGEAHTCGLDAGNNLYCWGYNYYGQIGNNLSGEGQQEETP
                     VLVYASAGLSFSAGRNHTCSIKTGDLYCWGQNTSGQLGTGNYDNVNQPVNVASTIEWS
                     FIEAGQNHTCGIDDADDLYCWGMNAEGQLGDGSINMKNTVQHIGTDKWSSVSAGYNHT
                     CGIKIGGTLLCWGGNGEGQLGDNGSNQNSNIPVAVDSMMVTSWKMVSAGSMHTCGISS
                     DDKLYCWGKNNYGQLGNGTIEPQNYPGELMGYGWADVSAGDGFTCAVKKSGSLHCWGL
                     NEFGQLGIGPVTGYHSDMQVISVKEDGN"
     gene            2686..2763
                     /locus_tag="PL056_03830"
     tRNA            2686..2763
                     /locus_tag="PL056_03830"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2721..2723,aa:Pro,seq:tgg)
     gene            2783..2859
                     /locus_tag="PL056_03835"
     tRNA            2783..2859
                     /locus_tag="PL056_03835"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2817..2819,aa:Arg,seq:tct)
     gene            2888..2962
                     /locus_tag="PL056_03840"
     tRNA            2888..2962
                     /locus_tag="PL056_03840"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2920..2922,aa:His,seq:gtg)
     gene            2973..3048
                     /locus_tag="PL056_03845"
     tRNA            2973..3048
                     /locus_tag="PL056_03845"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3006..3008,aa:Lys,seq:ctt)
     gene            3106..3188
                     /locus_tag="PL056_03850"
     tRNA            3106..3188
                     /locus_tag="PL056_03850"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3140..3142,aa:Leu,seq:tag)
     gene            3240..4544
                     /gene="tig"
                     /locus_tag="PL056_03855"
     CDS             3240..4544
                     /gene="tig"
                     /locus_tag="PL056_03855"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HPM46183.1"
                     /translation="MDYKLNHLSNVEKELEITFSAEEVKENIQGTLSHLSRTASIKGF
                     RKGKVPLNVVKQMYGKGIKDEAENHFVSTGIQETAVKENLKFATAPDIFEKSSVAEGE
                     PFIFKYRVEVFPSVNVEVKPFEAEYTPLKFKDEMVDQEIEEIRKRFVEFKESEDVPSA
                     EKDRMTITFSGKIGEEEIEGTKGNNVTVVLGEKRFIPDFEKALYGHKAGDSFSADVTF
                     PQDYQQKELAGKTVTFSIAIDKVEKCENEPELTDEFLAGKEGYPDTVEALREEMKKHI
                     LEYIENLNLDNKKYIAIDTYVKNHDIDVPPSILKSEVEARVDEQKKRNKVEEIAEDAL
                     KKIEEDAKWVAKRYIILHELSEKLGITVTERDINDALSKEAANYGLPAEYAEQLRKYY
                     GEERLNAKKMEIREGKVLEKITEAMIFIEKNTEEKKDEGKNF"
     gene            4525..5127
                     /gene="clpP"
                     /locus_tag="PL056_03860"
     CDS             4525..5127
                     /gene="clpP"
                     /locus_tag="PL056_03860"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013637631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HPM46184.1"
                     /translation="MKERTSEASLIPIVVEKEGGGERSYDIYSRLLKDRIIFLGDDVT
                     DDVANLIVAELLFLEAQDSEQDIQLYINSPGGSVTAGLAIYDTMQYVKPDVATICVGQ
                     ASSMGAVLMAAGAKGKRFSLPNSRIMIHQPMGGFRGQATDIEIHAKEILFLKERLLAI
                     LEEHTGKDRKTLKKDMERDFFMSAEEAVQYGLIDSVVKRK"
     gene            5137..6402
                     /gene="clpX"
                     /locus_tag="PL056_03865"
     CDS             5137..6402
                     /gene="clpX"
                     /locus_tag="PL056_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HPM46185.1"
                     /translation="MTQKDKNGVIQCSFCGKTQIEVERIIYGRGVYICNECVGLCSDI
                     LHEEGDSEIPQNEAGELLTPVQIKTRLDRFVIGQEYAKKVLSVAVYNHYKRVNAPKKI
                     KTKIQKSNILLLGPTGSGKTLLARTLAELLNVPFAIADATALTEAGYVGEDVENILLN
                     LIQAVNYDIEKAERGIIYIDEIDKIARKSENPSITRDVSGEGVQQALLKIIEGTVANI
                     PPTGGRKHPHQEYLKLDTSNILFIVGGAFEGIDKIISRRIGQKKLGFGSKPAVKKSEE
                     KILSNVQPEDLLKFGLIPEFIGRFPVTAYLDELNDSELLRILTEPEDALVSQYKELFK
                     LDGIDLSFTEDSLKEVVKKTGKLKTGARGLRRILETAMLDIMYTTPEEKDKIKEIIIT
                     PGVINSNDDPVKVIEKKTPVGTKSGNKGA"
     gene            6408..8789
                     /gene="lon"
                     /locus_tag="PL056_03870"
     CDS             6408..8789
                     /gene="lon"
                     /locus_tag="PL056_03870"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HPM46186.1"
                     /translation="MNSEKFFPLLPIRDAVVFPYTSITFKVGRDFSIKAVEESLNSKG
                     KEIVLITQKNPEMEIPDEKGMFKIGTLCSVLQVVEGPGDIFHVFIEGIRRVCIGEIRS
                     NIKGFFEVGTKVFKSKPVKKEEVEKERKLFEKTLTKYFEELDIPAGIISSTFAIKDDS
                     RFIDVSASIIRTSDPLDLQPVLESRDVRERFSRMTAILKMEMELRTLEADIESTVREK
                     FEKGQKEFFINEHINALKNELQKNSNFPDKESETLEARIDSLEAPKYVKERLEEELEK
                     YESISPVNPESSVIRNYIETLLALPWNTGTSSEIDLDKASEILENDHFGLEEVKKRIL
                     EYLAVLKLKGDMKSPIICLAGPPGVGKTSIAASLARAVGRKFIRQSLGGIKDEAEIRG
                     HRRTYVASMPGKIIQSIKKAKVNNPLFLLDEIDKLGSDYKGDPSSALLEVLDPEQNET
                     FTDHYIDVEFDLSKVLFVATANDVSMIPPALRDRMEIIWIDGYTWYEKKNIALVHLVE
                     KQKKLNGIENVVLEFTASGLNCLIDSYTSESGVRELERKIASICRKIALKKASTGGSP
                     LKRVTVSEKNIKDYLGVEKYNDDKIAKEKETGVVNGLAWTPYGGSVLKIEAIRYPGKG
                     DIKITGQLGDVMRESVEIAVSYIKSLSERALRIDKSLWDSSTIHVHFPEGATPKDGPS
                     AGVAIALAVGSMMSGLPVLPGIAMTGEITLRGKVLKVGGIRAKIMAAKKAGIKKVFIP
                     EANRDEYGELPLEIRDGIEAIMIKSCETVIMESLENLKKIKRSQAITKENVLQ"
     gene            8984..10234
                     /gene="rho"
                     /locus_tag="PL056_03875"
     CDS             8984..10234
                     /gene="rho"
                     /locus_tag="PL056_03875"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HPM46187.1"
                     /translation="MNLQELKNKKNDELVKMAEKLGIEDASGKRSQELIYEILQKLVE
                     NNEIILADGVLQILPDGFGFLRSPKYNYLPGPDDIYVPPSMIKKYGLRTGQTINGEIR
                     SPKDGERYFALQKITSVFENEDIDKNRHTPLFDNLTPFYPREKIVLETKSSVMSTRIM
                     DLLVPIGMGQRGLIVAPPRTGKTVLLQDIAHSITGNHPDVFLIVLLIDERPEEVTDMM
                     RTVDAEVIASTFDEPATRHVQVAEMVLEKAKRMVEFGKDVVILLDSITRLARAYNAIV
                     PPSGKILSGGVDANALHKPKRFFGAARNIEEGGSLTIIATALIDTGSRMDEVIFEEFK
                     GTGNMEIHLDRKLMEKRIFPCLDINKSATRKEELLLETDVLNKVWILRKLLHPLNVID
                     SMEFMLDKMGKTKSNKDFLKAMNS"
     gene            10380..12227
                     /locus_tag="PL056_03880"
     CDS             10380..12227
                     /locus_tag="PL056_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46188.1"
                     /translation="MKKILVSILVAVCFVMNLHAVADDSLLKSDETQKKYERKAVMGL
                     ADIGFMQNSINIPMQYCESSQSGYRCTPWSFTVNYPLAYDEKVKAAKEFYGWTITNRF
                     DKIILQNGNASMFTKNGEMTVETGEYLPYFSSGTDSLDGFKNRLENYLNSGVKESIGK
                     LLDATVETRYKELPETEQATFMVTKAKELGVSAEFVRALVNSSYVFAAHVQYIKGAGK
                     VIRKQIKTLDGRTIYTYDIEFDIPVNVTFLIYNYNFESKQFTLYKKLVAMSGMVSGST
                     DSFRIIPTHGQVMDAFNDTFQTAIKAASINANYQLKKDDNFALFMPVKAVNGSKIETD
                     FGVMEDIRVDHPFTVMESVDGQMKKKGYVRARKVSENCSDDYNKPTIMKITNGKAEEA
                     DLLREYPWTGLFFNLGLGMNSSLLTGIKDTNGALIDDIGGMFLSPGLHLGVSIDLGYS
                     TNSSALSEFYMDIFFQTSFGIDDSVDAGRPIWFGGGLGFSKRLYVGAGGFYIAPALNL
                     AYEGGATLNDYSMQQFILNPQLHFGFSVNPSFDVVLKGGWNLGFVVQVKDDLDRELDS
                     DLLAMGLTATLDFNFHLPIVGTMAKLYTKPSKVCAVKRKSEKKVEKETE"
     gene            12276..13490
                     /locus_tag="PL056_03885"
     CDS             12276..13490
                     /locus_tag="PL056_03885"
                     /inference="COORDINATES: protein motif:HMM:NF040342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6175 family protein"
                     /protein_id="HPM46189.1"
                     /translation="MKRMLVSLTVVISMLFASCGSAPVCNANTQPTPASYEASFVESS
                     GTGEMMIKATGLGCTMEEATNEAKKTAIWFVLEGGDKPILKSPAEKTKAVGLAQQMYA
                     NNAKYIRYQSEIKGKTKQGDKTKLTYLFKVDVAMITEELVASEIISSSEDIGEKIGMP
                     TISVFADKGGDYAKTAVVVIQEYFTDNSFEVYKADQGDKVNNLVSKISTLEGADTDPM
                     HDMALSLGSDIYAKINYSGGAGSMGTAKASVVVEVYETASGKMLGSTTGYSAERRVTD
                     PNALVQEATNDASSKILSQIKKEWMEMVKKGKPFKVTVLSNSNDGPAVDEAIYGALKN
                     MSPRPIKRQGGGKSTFSYIAYIKDVPNAYELFMNIKNNYSGPGKLEKVSDTGSFLVFK
                     ATSTGNIELTIE"
     gene            13499..14752
                     /locus_tag="PL056_03890"
     CDS             13499..14752
                     /locus_tag="PL056_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46190.1"
                     /translation="MKNIVLRIGIMFLIAVFLPVCLTAKGNYSKVKKDASDAFAELDG
                     SGPASPAPASSSKKGGKPSIPDVDVETAVLKGTGYGNSEKTAKMDALTQLSHSIVARI
                     KGETSLSQVEKDGKYTESLKNDITVNSAVFLKGVEFTAPKKVTQGYEVTALMSAQAVI
                     NTITYLITTLPDDLEALEPVKYDSILTTIYLAFSLLYSVPDSSVPEKKKYISMLSEVK
                     NEIERLASHGSIYFSGKTGLKGSVDINGTVNSINKKIFLKPGSYNFLIKADGYKNLKG
                     SFQISKGDKKFVELVLIPEKMAKKEVHLQVISEVRMVDDIEKQLLDFGIIPAQNEELP
                     HWIVVTLKGSTITVDNYRKYILEVDIHTFKNGQKYKITHYEHKPFFVTPQNEQMKIRE
                     ETKNVSLAVVKKFFSSINLEEFLSD"
     gene            14765..15658
                     /locus_tag="PL056_03895"
     CDS             14765..15658
                     /locus_tag="PL056_03895"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HPM46191.1"
                     /translation="MFNLRRNCLIIFAILLCSVFVYAQNIKVAVTDLDGGTYYKDYAS
                     TVTQIIISELSADSKIELVERSQLKKILDEHGLQMTGLVEPEKAVELGKFIAINKLIT
                     GTLGPLGNQFVITVRMIDIETAKIDLSVTETVPDASSLPSGSKVVAEKLLNAIKSGKA
                     VSESAREKLKYSCETKKDATACFKLGVSWYKGIEGMKNLIFAKNHFATSCSYGHGRAC
                     YYTGNMLKKGIGGNKELLNSGKYFKAACDSGFQKGCTQKGFSSGGSSFGAPATGGGNE
                     SIGPKKKPSSGSEETISELEE"
     gene            15679..>15938
                     /locus_tag="PL056_03900"
     CDS             15679..>15938
                     /locus_tag="PL056_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015775010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HPM46192.1"
                     /translation="MDLSVKIGKMTLQNPILTASGTFGYGPEFSYYLDLNKIGGFCTK
                     GISIEPRGGNPAPRIVETASGMLNSIGLENCGSEAFLTKVMPT"
BASE COUNT         5110 a         2889 c         3775 g         4064 t
ORIGIN      
        1 aggagttata aatgagaatt ctgatctttt caattttgct gataccgggc ttgctttttg
       61 ccgacaagat agttgtctgg aacttaaaac cgcaggcggg agttactgaa aaggaagcgg
      121 cgacagttag ttcgatagtg acgactgaaa tagcgagagt ttccaaaagc acgatcatca
      181 gtgaatcaga gatgaagtcg gtaatagatg ccgaacagat gaaaatgtca tgtggagcgg
      241 aagactcttc atgtatagca gagataggtg ccgcgatggg tgcgccatta tcggtttccg
      301 gaacattttc aaaaatggga gattactgga taatcactct tcagcttgtc gatgtgagaa
      361 aagttgaggt tcagtcaagg gtttcaaaga agttcaaagg gaatgagaat gcccttgtcg
      421 aagcgataac tccgatgatc tgcgagctgt ttaacgacaa ggaatgcatg gaaaaaagtg
      481 ttcagcagac aaaagaggat cagaagaacg aaaaaaagga gaaaacccct gtttcatgga
      541 aaaaagttac cggctgggtc ctcacggttc cgggagtatt gatgttcggg tcaggtgttg
      601 cggcacatat tctgatgaaa aatgcaaaag aagattatga attgactggc gttgatgaag
      661 ggagatacaa aagtttcaga acatattcaa ctgtcggcta tgtcaccggc tcggttctca
      721 tggcgggtgg aattgcactg ctgatctggg acggagttga aaaaagcaag aaaaataaga
      781 aaaatgaagc ggtgtcattt tatgtaatcc cttcatttga cggtggttat gcaggaatgc
      841 aatggagctg gtgatgaaga aaatattttt tgctgtgact gttttcttaa tgttttcggg
      901 ttgttctgac ttcaaagaca gtttgatcgg aactgttggt gaaaagtgtt tcgggaacgg
      961 aacatgcaag gatggtctaa tatgtattga aggaaaatgc gccaaggatg aagaagccga
     1021 aaatgatgac gattcgggta acaccggaga caccggnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnacac cggtgacgat gatgatgttt caaatgtcga aacggaagta
     1201 gatgatgatg tcattaatta tccttgtgac ccgaatccct gtgcaactct cctgaattcg
     1261 accggagagt gctctcctga tgttgatgac agtcatgtct gtacatgcaa tgacgggttt
     1321 gaatgggttt ttgaagataa ggtatgcaac gccattcccg acccgtgtct taatgacccc
     1381 tgtacaactt tggctgattc aacatgtgtt cctgaaaaaa cagaagatca gcagaatttt
     1441 accggaatgc ttgaatgtgt ctgcaatgaa ggatttgaca agagtttcat aggttcagag
     1501 atctgtgttc cggcttttaa atcggtttcg acagggaaag atcacgcctg tgcagttgat
     1561 tattatgaca aactgtactg ctggggttca aactttaacg cgcagctagg caattcatta
     1621 acaggaacag gagaaggatg cctggcagag caccctttta aagtcgatga taactcatgg
     1681 actgctgttt cagccggaga agcacacacc tgcggattag atgccgggaa caatctttac
     1741 tgctggggat acaattatta cggacagatc ggtaacaacc tgtcaggcga aggtcagcag
     1801 gaggaaacac ctgttcttgt ctatgcaagt gccggtttga gtttttctgc cggaagaaat
     1861 cacacatgtt caataaaaac aggggatctt tactgctggg gacagaacac aagcggtcag
     1921 cttggaaccg gaaactatga taatgtgaac cagccggtca atgttgcttc aacaattgaa
     1981 tggagtttta tagaagcagg acagaatcat acatgcggga tcgatgacgc agacgatctt
     2041 tactgctggg gaatgaacgc tgagggacag cttggcgacg gatcaataaa tatgaaaaat
     2101 accgtgcagc atatcggaac tgataaatgg agctctgttt ctgcgggcta taatcataca
     2161 tgcggaatta agataggcgg aactctttta tgctggggtg gtaacggtga aggtcagctt
     2221 ggagataacg gatccaatca gaactctaat attcctgttg ctgtggattc gatgatggta
     2281 acatcatgga aaatggtttc tgcaggaagt atgcacacct gcggaatttc gtcagatgac
     2341 aaactttact gctggggaaa gaacaattac ggccagctcg gaaacggaac aatagaacct
     2401 cagaactatc ctggagaact tatgggctac ggatgggcag atgtttcagc cggtgatgga
     2461 ttcacatgtg ctgttaaaaa gagcggttca ttgcattgct ggggattgaa tgaatttggt
     2521 cagcttggta tcggtccagt cacaggatat cattctgaca tgcaggtgat aagtgtgaag
     2581 gaagacggta attagccgta atcattgttt aatttatgtg ccaatttctc aaaatcgtta
     2641 aaaaacttga ttttgaccag tttgtttata atatccttcg tgcttcgggg tgtagcgcag
     2701 tccggttagc gcatcggttt tgggaaccga gggtcggagg ttcgaatcct ctcaccccga
     2761 ccatgtgact ttttttggga gtgcgcccgt ggctcagttg gatagagcaa cggacttcta
     2821 atccgtaggt cagaggttcg aatcctctcg ggcgtaccac ttgttttttt acatgtgaat
     2881 tatcgcggtg ggtgtagttc aatggtagag caccggattg tggttccggg cgttgtgggt
     2941 tcgagcccca tctcccaccc cattttttta gtgggccctt agctcagttg gtagagcaac
     3001 tgactcttaa tcagtgggtc gaaggttcaa atccttcagg gcctaccact tttttcacgg
     3061 tcttagatgg cagattcaat taaacggtat tttaaaaatg atactgcgaa ggtggcggaa
     3121 ttggcagacg cactagactt aggatctagc gggcgaccgt gggggttcga gtcccccccc
     3181 tcgcaccaga ctgaaaccgg tgatcacttg aaaacagcta attaatacga ggagttaaaa
     3241 tggactacaa attgaatcat ctcagcaatg tggaaaaaga acttgaaata acattttctg
     3301 cagaagaagt taaggaaaac attcagggca cactttcaca tctttcaaga acagccagca
     3361 taaaaggttt cagaaaaggg aaagtcccct tgaatgtggt taagcagatg tacggaaaag
     3421 gcattaaaga tgaagccgag aaccatttcg tatcaacagg cattcaggaa actgcggtaa
     3481 aggaaaacct caagtttgca acagcacctg atatttttga aaaaagttcc gtagctgaag
     3541 gcgagccgtt catattcaaa taccgtgttg aagttttccc gtccgttaat gttgaagtga
     3601 aaccgtttga agctgaatat actcctttaa agttcaagga cgagatggtc gatcaggaaa
     3661 ttgaagagat cagaaagaga tttgtggagt tcaaggaaag tgaagatgtg ccatcagccg
     3721 aaaaagaccg catgacgata actttttcag gtaaaatcgg tgaagaggag atagaaggga
     3781 caaaaggaaa caatgtaaca gttgttcttg gtgaaaaaag attcattccc gattttgaaa
     3841 aggcacttta cggacataaa gcaggtgaca gtttttcggc tgatgtgacc tttccacagg
     3901 attatcagca gaaagagctt gcagggaaaa ctgtaacatt ttcaattgcg atagacaaag
     3961 ttgaaaaatg tgagaacgaa ccggagctta cagatgaatt tcttgcagga aaagaagggt
     4021 atcccgatac cgttgaggca ctaagggaag agatgaaaaa acacatcctt gaatatattg
     4081 aaaatcttaa cctggacaat aaaaagtata tcgcaattga cacctatgtt aagaaccacg
     4141 atattgatgt tcccccttca attttgaaaa gtgaggtcga agcaagagtt gacgaacaga
     4201 aaaaaaggaa taaagttgaa gagatagctg aagatgcttt aaagaaaata gaagaagatg
     4261 caaaatgggt tgcaaagagg tacataattc ttcacgagct ttcagaaaaa ctcgggataa
     4321 ctgttactga aagggatatt aatgatgctc tgtcgaaaga ggcggcaaat tacggacttc
     4381 ctgcagaata tgctgaacag cttagaaaat attacggtga agagcggctg aatgccaaga
     4441 aaatggagat aagggaagga aaagtgcttg aaaaaattac agaagcgatg atatttattg
     4501 aaaagaacac tgaggagaaa aaagatgaag gaaagaactt ctgaagcatc acttatcccg
     4561 atagttgttg aaaaggaagg gggtggagag agatcttatg acatctattc ccgtctttta
     4621 aaggaccgta taattttcct tggggatgat gtgacagatg atgttgccaa tctaatcgtt
     4681 gcggaacttc tctttcttga agcgcaggac agcgaacagg acatccagct ttatatcaac
     4741 agccccggtg gttcggttac agcaggtctt gcaatatacg acaccatgca gtatgtgaag
     4801 cccgatgttg caacgatttg tgtcggccag gcatcgagca tgggtgctgt gcttatggcg
     4861 gcgggagcga aggggaaaag attttcgctt cccaattcaa ggataatgat ccatcagccg
     4921 atgggtgggt tcagaggaca ggcgacagat attgaaattc atgcaaaaga gatcctgttc
     4981 cttaaagaaa gacttcttgc aattcttgaa gaacacaccg gaaaggacag aaagactctg
     5041 aaaaaggata tggaaagaga ttttttcatg tctgccgagg aagccgttca gtacggactt
     5101 attgacagcg tggtgaaaag aaaataggag gatgaaatga cccagaagga caagaacgga
     5161 gttattcagt gctctttctg cggcaaaacg cagatcgaag ttgaaagaat aatatacgga
     5221 cggggagttt atatctgcaa tgaatgtgtg gggctgtgct cggatattct tcatgaagaa
     5281 ggtgacagcg agatcccgca gaacgaggca ggagaacttc ttactcccgt tcagataaag
     5341 acccgtcttg acagatttgt gataggtcag gaatatgcca aaaaagtcct ttctgtagca
     5401 gtttacaacc attataaaag ggtcaacgcc ccgaaaaaaa tcaaaacgaa gatacagaag
     5461 agcaatattc ttcttctggg gcctaccggt tcaggtaaaa ccctgcttgc aaggacactt
     5521 gccgaacttc tgaatgttcc ttttgcgata gctgatgcaa ctgctctgac cgaggcggga
     5581 tatgtaggtg aagatgttga gaacattctg ctgaatctga ttcaggcggt caactatgat
     5641 atcgaaaagg cggaaagagg gatcatatac attgacgaaa tagataagat cgcaagaaaa
     5701 tctgaaaacc cttcgatcac aagagatgtg agcggtgaag gtgttcagca ggcgttactg
     5761 aaaataatcg aaggaactgt tgcaaacatt cccccgacag gcggcaggaa gcatcctcat
     5821 caggaatatc tgaagcttga tacttcaaac attcttttca tcgtcggcgg agcttttgag
     5881 gggatagaca aaataatatc aagaaggatc ggtcagaaaa aacttggatt cggttcaaaa
     5941 cccgctgtga aaaagtctga agaaaaaatt ttgtcaaatg ttcagccgga agatcttctc
     6001 aagttcgggc ttattcctga gtttataggc agatttcctg tcactgcata tcttgacgaa
     6061 cttaatgaca gtgaactttt aagaatactt accgagcccg aagatgcact tgtaagtcag
     6121 tataaagagc tattcaagct tgacggcatt gacctttcgt tcacggaaga ttccttgaaa
     6181 gaagttgtaa aaaagaccgg aaagctcaaa acaggggcaa ggggattaag aagaatactt
     6241 gaaacggcga tgcttgacat aatgtataca actccggaag aaaaggacaa gatcaaagag
     6301 ataataatca ctcccggcgt tatcaattca aatgatgatc cggtaaaagt cattgaaaag
     6361 aaaaccccgg tcggtacaaa aagcggcaac aaaggagcct gacacgaatg aattctgaaa
     6421 aattttttcc actgcttccg ataagggatg cagtagtgtt tccatataca tccataactt
     6481 tcaaggtcgg cagggatttt tccataaagg cagttgaaga atccttgaac agcaaaggga
     6541 aggaaatagt tctcattaca cagaaaaatc ccgaaatgga gatccctgat gaaaaaggaa
     6601 tgttcaagat aggaactctc tgttcggttt tgcaggtagt tgaagggccc ggcgatattt
     6661 tccatgtttt catagagggg ataagaagag tctgtatcgg agagataaga tctaacataa
     6721 aaggtttttt cgaggttgga acaaaggttt tcaaatctaa accggtaaaa aaggaagaag
     6781 ttgaaaaaga gagaaagctt tttgaaaaga cactcacaaa atattttgaa gaacttgata
     6841 ttccggcagg gataataagc agcactttcg ccattaagga tgattcaagg ttcatagatg
     6901 tctcggcttc aataatcaga acatcagatc cactcgatct acagccggtt cttgaatcga
     6961 gggatgtcag agaaaggttc agcaggatga ccgcgatact caaaatggaa atggagctga
     7021 gaactcttga agctgacata gagtcgactg tcagagagaa atttgaaaaa ggacagaagg
     7081 aatttttcat aaatgagcat atcaacgcac tgaaaaatga acttcagaag aattcaaatt
     7141 ttccggataa ggagagcgaa actcttgaag cgaggataga ttcgctggag gcacctaaat
     7201 atgtcaagga acggctggaa gaggagttgg aaaaatatga atcaatatct cccgtaaatc
     7261 cggaaagttc cgtcatcaga aactatatcg aaacgcttct tgcccttccg tggaacacag
     7321 gaacttcatc tgagatcgat cttgataagg catctgagat acttgaaaat gaccacttcg
     7381 gacttgaaga agtaaaaaag agaatacttg aatatcttgc agtgctcaaa ttgaaaggag
     7441 atatgaaatc accgattata tgtcttgccg gccctcccgg tgtgggcaaa acctcaatag
     7501 cggcatcatt ggcaagagct gtgggaagaa aattcatcag acagtcgctt ggcggaataa
     7561 aggatgaagc cgaaataaga ggtcacagaa gaacatatgt cgcttcgatg cccggcaaga
     7621 taatccagtc gataaaaaag gcgaaagtga ataatcccct tttccttctt gatgagatag
     7681 ataaactcgg aagtgactac aagggagacc cgtcaagtgc tcttcttgaa gttcttgatc
     7741 ctgaacagaa tgaaactttc actgatcact atattgatgt ggaatttgat ctgagcaaag
     7801 tcctttttgt cgctactgca aatgatgttt caatgatacc tcctgctctg agggacagga
     7861 tggagataat ctggatcgac ggttatacct ggtatgaaaa aaagaacata gctctcgttc
     7921 atcttgtcga aaaacagaaa aagctcaacg ggatcgaaaa tgttgttctt gaatttacag
     7981 cttcgggtct taactgcctg attgacagtt acacatcgga atcgggggtc agggaactcg
     8041 aaagaaaaat tgcctcgata tgcaggaaga tcgctttgaa aaaagcttct accggaggga
     8101 gccctctgaa aagagtgact gtttcagaaa aaaatatcaa agattatctt ggtgttgaga
     8161 aatataatga cgacaagata gcaaaggaaa aggagaccgg tgtggtgaac ggtcttgcgt
     8221 ggacccctta cggaggaagt gttctcaaga ttgaagcgat aagatatccg ggtaaaggtg
     8281 atataaagat aactggacag cttggcgatg taatgaggga atctgtggaa attgcagtaa
     8341 gttacattaa gagtctttct gaaagagcac ttcgtataga taagagtttg tgggacagtt
     8401 cgaccattca tgtccacttt cctgaagggg cgacacccaa agacggtcca tctgccggcg
     8461 ttgcgatcgc acttgctgtg gggtccatga tgtcgggact tcctgtcctt ccgggtattg
     8521 ccatgacagg ggaaataacg ctgaggggca aagttcttaa agtcggcggc ataagggcaa
     8581 agatcatggc ggcaaagaag gcggggataa aaaaggtgtt cattccggaa gcaaacaggg
     8641 atgaatacgg ggaacttcct cttgaaataa gggacggaat tgaagcgata atgataaaat
     8701 catgcgaaac cgtaatcatg gaaagtcttg aaaatttgaa aaagatcaag cgatctcagg
     8761 caattacaaa agaaaatgtt ttacagtgat caaatatatt tgacaaaacg gctaaaatgg
     8821 ttaattaact gaatgtgcat ttcgttcaac tcaaaaattt tgagtacagt cagcaaaaaa
     8881 tctgcaaatg atttaccgga agggccgtgg atggtgtctt gcaaaatttt aaatttgaat
     8941 catacacaaa ataggatact ttattaactt aatggagaaa agaatgaatt tacaggaatt
     9001 aaaaaacaaa aagaatgatg aactggtcaa gatggccgaa aagctcggta ttgaagatgc
     9061 ttcaggcaag cgctcgcagg aacttattta tgaaattctg cagaaactgg ttgaaaacaa
     9121 tgagattatt ctggcagacg gcgtgttgca gatccttcct gacggtttcg gatttttaag
     9181 aagcccgaaa tacaactatc ttccgggtcc tgatgacata tatgttccgc catcaatgat
     9241 aaaaaaatac ggtctccgta ccggtcagac aataaacggt gagatcagaa gccccaagga
     9301 cggtgaaaga tatttcgcac ttcagaagat aacatcagta tttgaaaatg aggatattga
     9361 caagaacaga cacacaccgc tttttgacaa tctcacccct ttctatccca gagagaagat
     9421 agttcttgaa acgaagtcga gcgtaatgtc aacaaggatc atggaccttc ttgttccgat
     9481 cggaatgggt cagagaggtc ttattgttgc tccgcctaga accggaaaaa ctgttcttct
     9541 tcaggatatt gcacattcca tcacagggaa ccatcctgat gttttcctta ttgttcttct
     9601 cattgatgaa agacctgaag aggttaccga tatgatgaga actgttgatg ccgaagttat
     9661 tgcctcaaca tttgatgaac ccgcaacacg ccatgttcag gttgctgaaa tggttcttga
     9721 gaaagcaaag agaatggttg aattcggcaa agatgttgtc attcttcttg actcgataac
     9781 acggcttgca agggcttaca atgcgatcgt tcctccgtca ggcaagatcc tttccggtgg
     9841 tgttgatgca aacgctcttc acaaaccgaa aagattcttc ggcgcggcaa gaaacattga
     9901 agaaggcggc tctctgacaa ttatcgcaac agcccttatt gatacaggaa gtcgtatgga
     9961 cgaagtaata tttgaagagt tcaagggaac cggtaacatg gaaattcacc ttgacagaaa
    10021 actcatggaa aagagaatat tcccatgtct tgacatcaac aagtctgcaa ccagaaaaga
    10081 agaacttctt cttgagacag atgttctcaa taaggtatgg atacttagaa aacttctcca
    10141 tcctctcaat gtaatagatt caatggaatt catgcttgac aagatgggca agacaaaatc
    10201 caacaaggat ttcctcaaag cgatgaattc ctagtatttt caaatacata tcaatttaat
    10261 gcccgcttgt aacgagcggg cttttttatt taattaagtt aatgttataa aatttgattt
    10321 ttggggctaa tatgagataa tcggccaagt gaattttagg ggtttttcag ggggtagata
    10381 tgaaaaagat tctggtttca attttagtag cagtatgttt tgtaatgaat cttcatgcgg
    10441 ttgcagatga ttctttatta aaatcggatg aaacgcagaa aaagtacgaa aggaaggcgg
    10501 tcatgggact tgccgacatc ggattcatgc agaattcgat caacattccg atgcagtatt
    10561 gcgaaagcag tcaaagcggt taccgctgca ctccgtggtc attcacggtc aattatccgc
    10621 ttgcctatga tgaaaaggtc aaagcggcga aagaattcta cggatggacg ataactaacc
    10681 gttttgacaa gataattctt caaaacggca acgcaagcat gttcacaaag aacggtgaaa
    10741 tgactgtcga aacaggcgaa tatcttccat atttttcttc gggaaccgac tcacttgacg
    10801 ggtttaagaa ccggcttgag aattatctga acagcggagt caaggagagt atcggcaagc
    10861 ttcttgatgc aactgtcgag acaagataca aggaacttcc tgagaccgag caggcaactt
    10921 tcatggtgac taaagcgaaa gagcttggtg tttcagcgga atttgtaaga gcgcttgtaa
    10981 attcgtccta tgtttttgcg gcccatgtcc agtatataaa aggtgcaggg aaagttatca
    11041 gaaagcagat caaaactctg gacggaagaa caatttatac atacgacatc gagtttgata
    11101 ttccggtaaa tgtcacattt ctcatctata attacaactt tgaatcaaag cagttcacac
    11161 tttataaaaa acttgttgca atgtctggaa tggtttccgg atcaacagat tccttcagga
    11221 taattcctac tcacggtcag gtcatggatg ctttcaacga cactttccag actgcgataa
    11281 aagcggcttc gatcaatgcg aattatcagc tcaaaaaaga tgataacttc gcccttttca
    11341 tgccggtcaa ggcagtaaac ggttcaaaaa ttgagaccga tttcggtgtt atggaagata
    11401 tcagggtcga tcatcctttt acagtaatgg aatctgttga cggacaaatg aaaaagaaag
    11461 gttatgtgag agcacggaaa gtaagcgaga actgctcgga tgactataac aagccgacaa
    11521 taatgaagat cacaaacgga aaagctgaag aagccgacct tttgcgagag tatccgtgga
    11581 caggtctttt cttcaatctc ggtctgggaa tgaattcatc acttcttaca ggcataaaag
    11641 acacaaatgg tgctttaatt gatgatatcg gtggaatgtt cctttcgccc ggactccatc
    11701 tcggggtgag catagatctt ggttattcaa caaacagttc tgcactgagc gaattttata
    11761 tggatatttt ctttcagaca agtttcggaa ttgatgattc tgttgatgca ggtcgtccga
    11821 tctggtttgg cggcggactc ggtttttcaa aaagattgta tgtcggagca ggcggttttt
    11881 acatcgctcc ggctttaaac cttgcttatg aagggggagc aacactcaat gattattcaa
    11941 tgcagcagtt cattttaaac cctcaacttc atttcggttt cagcgttaat ccgtcttttg
    12001 atgttgtttt aaaaggtggc tggaatctcg gatttgttgt tcaggttaaa gacgatcttg
    12061 atagagaact tgacagtgat cttcttgcaa tgggcttaac tgctacactt gattttaact
    12121 ttcaccttcc gatcgttgga actatggcaa aactttatac aaagccgtca aaggtatgtg
    12181 ctgtaaaaag aaaatcagaa aaaaaagttg aaaaagaaac tgaatagggc gtttatataa
    12241 ataggtttat ttttaatttc tgggaggaaa tcaaaatgaa aagaatgtta gtaagtttaa
    12301 cagttgttat ttcaatgctt tttgcaagct gcggctcagc tccggtctgc aatgcaaaca
    12361 ctcagccgac tccggcaagt tatgaagcaa gttttgttga aagttcagga acaggcgaaa
    12421 tgatgatcaa ggcgactggt cttggatgca caatggaaga agcgacaaat gaagcaaaga
    12481 aaaccgcaat atggttcgtt cttgaagggg gagataaacc catactgaag tctcctgcag
    12541 aaaagacaaa agcagtcgga cttgctcagc agatgtatgc aaacaatgca aaatacatca
    12601 gatatcagtc agagatcaaa ggaaagacaa aacagggtga caagacaaag ctcacttatc
    12661 ttttcaaagt tgatgtggca atgataaccg aagaacttgt tgcaagcgag atcatctcat
    12721 cgtctgaaga tatcggtgaa aagatcggaa tgcccacaat ttcagttttt gccgacaaag
    12781 gcggtgacta tgcaaaaacc gcagttgtag taattcagga atatttcaca gacaattcat
    12841 ttgaagttta taaggcagat cagggcgaca aggtcaacaa tcttgtcagc aagatctcta
    12901 cgcttgaagg tgccgacaca gatccgatgc acgatatggc cctttcactt ggaagcgata
    12961 tctatgcaaa aataaattat tctggcggtg caggaagcat gggaacagca aaagcttcag
    13021 tagttgtgga agtttatgaa actgcctcag gcaaaatgct cggttccacg accggttatt
    13081 cagcagaaag aagagtgacc gacccgaatg cacttgttca ggaagcgaca aatgatgctt
    13141 cttcaaaaat attaagccag atcaagaaag agtggatgga aatggttaag aagggcaagc
    13201 cattcaaagt tactgtactt tcaaattcaa atgacggccc ggcagttgat gaagcaatct
    13261 acggagcgct caagaacatg tcaccaagac caataaaaag acagggtggc ggaaaatcta
    13321 cattcagcta tatcgcctac ataaaagatg ttccgaacgc ttatgaactt ttcatgaaca
    13381 ttaagaacaa ctacagcggc ccgggtaaac ttgaaaaagt aagcgatacc ggctcattcc
    13441 ttgttttcaa agcaacaagc acaggtaaca ttgaattaac aattgaatag gtcaagtaat
    13501 gaaaaatatt gttttgcgca tcggaataat gtttctgatc gctgtttttc ttccagtctg
    13561 cttaacggca aaagggaact acagcaaagt gaagaaagat gcctccgatg cttttgcaga
    13621 acttgatgga tcggggccgg caagtccggc tcccgcatct tcatcaaaaa aaggtggcaa
    13681 accctctatt cccgatgttg atgtcgaaac agccgtttta aagggaacgg ggtatggcaa
    13741 tagcgaaaaa actgcaaaga tggatgctct cactcaactc agccattcga tcgttgcgcg
    13801 gatcaagggt gaaacatctc tttctcaggt ggaaaaagat ggcaagtata cagaatcatt
    13861 gaaaaatgac atcacagtca actctgctgt tttccttaaa ggtgttgaat ttacagcccc
    13921 taaaaaagtg acacagggtt atgaggtcac tgctttaatg tcagcgcagg cggtaataaa
    13981 tacaataact tatcttataa caactctgcc ggatgacctt gaagcgcttg agccggttaa
    14041 atatgattca attctcacaa cgatatatct cgctttttcg ttgctttatt ctgtgcctga
    14101 cagttctgtt cctgaaaaga agaagtatat cagcatgtta agcgaagtta aaaatgaaat
    14161 tgaacggctt gcttcacatg gctccattta tttttcaggt aaaacgggtc ttaaagggag
    14221 tgttgatata aacggaactg taaacagcat caataaaaag atattcctga aacctggcag
    14281 ttacaatttc ttaataaaag ccgacggtta caaaaacctt aaaggttctt tccagatctc
    14341 aaaaggggac aagaaattcg ttgaactggt gcttattcct gaaaaaatgg caaaaaaaga
    14401 ggttcatctt caggtgataa gcgaagtgag aatggttgat gacattgaaa agcaacttct
    14461 tgatttcggg ataattcctg ctcagaacga ggagcttccc cactggatag tcgtaactct
    14521 taaaggaagc acgataactg ttgataatta cagaaaatat attcttgaag ttgacatcca
    14581 cacattcaag aacggtcaga aatataagat cactcactat gaacacaagc cgttctttgt
    14641 aactcctcag aatgaacaga tgaagataag agaggagaca aaaaatgttt ctcttgctgt
    14701 cgtaaaaaag tttttctcat caataaatct tgaggaattc ctctcggatt aaacggaggc
    14761 gatcatgttt aatttaaggc gcaactgctt gataatattt gcaatattgc tttgctccgt
    14821 gtttgtgtat gctcagaata tcaaagttgc tgttacagac cttgacggtg gaacttacta
    14881 caaagattat gcttccacag ttacccagat aataatttct gagctttccg cagattcgaa
    14941 gatcgagctt gttgaaagaa gtcagcttaa aaagatcctt gatgaacacg gtttgcagat
    15001 gactggtctt gttgagccgg aaaaagctgt ggaactggga aaattcattg cgataaacaa
    15061 gcttataaca ggaacgctcg ggccgcttgg caaccagttt gtaataactg tcagaatgat
    15121 cgatatagaa acggcaaaga tcgatctttc ggtaactgaa actgttcctg atgcctcttc
    15181 gcttccatcg ggctcaaaag ttgttgctga aaaactgctt aatgcgataa agagcggaaa
    15241 agcagttagt gaaagtgcca gagaaaaact aaaatatagt tgcgaaacaa aaaaagatgc
    15301 gactgcatgc tttaagcttg gagtttcatg gtataaagga attgaaggga tgaaaaacct
    15361 gatatttgca aaaaaccatt ttgcgacttc ttgcagttat ggacacggaa gggcttgtta
    15421 ttacacagga aatatgctta aaaaaggaat cggcgggaac aaggaactac ttaattcggg
    15481 taaatacttt aaagccgcat gtgattcagg atttcagaaa ggatgcacgc aaaaaggttt
    15541 ttcttccggc ggatcgtctt tcggagcacc tgcgacagga ggaggcaatg aatcgatcgg
    15601 tcctaaaaag aagcccagca gtggatcaga agagacaata tctgaacttg aagagtgaca
    15661 caataggaga caaattcaat ggacctgtct gtaaaaatcg gcaaaatgac tctgcaaaat
    15721 ccaatactta ccgcttcagg cactttcggg tacgggcctg agttctcata ttatcttgat
    15781 ctgaataaga tcggcggttt ctgcacaaaa gggatttcaa ttgagccccg tggcggtaat
    15841 cctgctccga gaattgttga aacagcttcg ggaatgctta actcgatcgg gcttgaaaac
    15901 tgcggatcgg aagcgtttct tacaaaagtg atgccgac
//
LOCUS       DAOOYB010000041        15854 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_3635_length_15854_cov_11.171277, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000041 DAOOYB010000000
VERSION     DAOOYB010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 15854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15854
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3635_length_15854_cov_11.171277"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..1253)
                     /locus_tag="PL056_03905"
     CDS             complement(84..1253)
                     /locus_tag="PL056_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007277337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HPM46193.1"
                     /translation="MTQKDFSKLIEAVKIVEEFGFEGLEEALTLIFNEAMKAERSRAL
                     GAERYERTESRKGYANGFKPKTVNSRLGRLELEIPQVRGEIEFYPSALEKGLRSERAL
                     KIVMAEMYVQGVTTRKVRKVFEKLCGVEVTSMEVSRAAKLLDEELEKWRNRPLGEIPY
                     VQFDARYENVRMNGSVVSAATLIAVGVRKDGKRTVIGVSTSVSEAEIHWRKFFESLVK
                     RGLHGIKMITSDDCPGLKTAMKAVFTGVPWTRCHVHLQRNSFKYIPKQAMRDEVSRTI
                     SGILNAPDLHEAQRQLAKAVDHYEKKAPKLAEWMDTNIPEGFAIFQLHHKLRRKLRST
                     NLLERLNREIKRRTRVSTLFPNEESILRLVSAILMEQSEEWETGICYINLEDCSD"
     gene            complement(1884..2375)
                     /locus_tag="PL056_03910"
     CDS             complement(1884..2375)
                     /locus_tag="PL056_03910"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HPM46194.1"
                     /translation="MKRVLILSTIFFLSVAMVSCGGEDKCPDSNKFCYEYASLNWSDI
                     SSNSMYWDAAVTYCENIGGRIPTISELRTLIQNCPATETGGECGVTDDCLSNIDCYSL
                     SICSGFDSDDSNEYSIFGDKKILWSSSEVPDKDYVYLVDFEFGRVFYYDKISEPNNVR
                     CVR"
     gene            complement(2674..3858)
                     /locus_tag="PL056_03915"
     CDS             complement(2674..3858)
                     /locus_tag="PL056_03915"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM46195.1"
                     /translation="MQKLLNTFNPWWDDGYLFQMQKRDKYLNRIFQHMESRDIILLAG
                     LRRVGKTSIMKLIIGELISKGFDKTRILFVSMDDFLFNEKSIIDIIAEYRLLHKIRME
                     EKIHLFFDEVTYKDKWQQQLKNLYDKENVKIIASSSSSSIFIDENAYLTGRSRIIEVK
                     PLDFDEWIEFKDIKLKAVDKDLMVTYFDEYLSDGGMPEFVLTKDRAYLQNLVEQLIYK
                     DIIARHNIKMKSVIKDLFLLLMERSGKQLSINNIAKILNITPSNAKRYIEYFEETFLI
                     GLIKKYGKTNELITSQNKLYSGDIGIRNAYTGYRDKGAVFENYIYLKIKEKKPSYYVE
                     DGIEIDFITDRYSGSQYLIECKYNSELTEKQKKVFEKAAVKNKIILNGYVDLHLLKKL
                     EN"
     gene            complement(3973..6300)
                     /locus_tag="PL056_03920"
     CDS             complement(3973..6300)
                     /locus_tag="PL056_03920"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPM46196.1"
                     /translation="MVNFFNFRTGYRVMAVAVLMTVFFSLNSESFVSIHGYPQEKPEV
                     PDYQFQTQTTMIINQTIDRILYPVIGFPAMVTAVDPEMSFIIRSENIPEIDHVRIIRE
                     IGDNVKEFQDLIPYSVEQCGFGLYKVKMIVPVILPSVRYDLSVKLKGEEMPIISRNSV
                     FFPEFDASTKFFIWADPQIEDLQSKMAGDLNYNSGEYPYKSDSILDFSRQAGIIKTTI
                     SNLNMGDRHFVTMLGDIVFGINYQREYEDILSLIVNLEIPFFPVPGNHDGYAKFTEQN
                     NLFSPLDWDGLQYWTKFVGPLHYAFNFNGQTFLMLNTYDGTPQRRAAGDALGIGDNAA
                     VPVSNWGGFLTDRSLAWIEIMMDDYDVFGLFSHMTPLGQNATGKYHKMKKFPKDSVIG
                     VTDSQEWNIETSEYDSDPTDLIFNETEKINTGIKLAELITRQFPPPIYFSGHTHKDRL
                     YLFEKDSELVDGSGVYAKDDMEFIMTTTAATSGQLYWGFRKVDVGVTGDVSYNYTCER
                     GVNCMPGSETEKGFQSVPAGNMWVTYKWNSFGNETESIFAGGDGSADSVSAEVMNYLP
                     TEEPVTLRFFMPAGKSYKIENPKFHISNAAVSKDMTTLILTVKGSIAAGSEMDKFLAK
                     DFSRKVEFVTVTPSTATVVEPQIEYPSSIFEDEPLSAEVKNGDDFLSLVWIRNKIEFA
                     QGKSFSVKFDNYQNVETIFLIYIDKSGVHGTALFKTDIQKRPEEEPDEEVSEEIEEIS
                     DPDMIEIDEIQEDSDEDSDTQEAKKKKSGCSVTVI"
     gene            complement(6410..6505)
                     /gene="ffs"
                     /locus_tag="PL056_03925"
     ncRNA           complement(6410..6505)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PL056_03925"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(6518..6607)
                     /locus_tag="PL056_03930"
     tRNA            complement(6518..6607)
                     /locus_tag="PL056_03930"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6571..6573),aa:Ser,seq:gga)
     gene            complement(6630..6721)
                     /locus_tag="PL056_03935"
     tRNA            complement(6630..6721)
                     /locus_tag="PL056_03935"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6685..6687),aa:Ser,seq:cga)
     gene            complement(6724..7227)
                     /gene="tadA"
                     /locus_tag="PL056_03940"
     CDS             complement(6724..7227)
                     /gene="tadA"
                     /locus_tag="PL056_03940"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008636096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HPM46197.1"
                     /translation="MEREISYMKQALELAETAGSQGEVPVGAVVVFENRVIGKGYNRR
                     ESTNSPLSHAEIEAIMEASENMGSWRLEDCELFVTLEPCIMCSGAILQSRIKRVVFGP
                     RDPKAGAVRSLYNLLEDTRLNHRVEVVEGVLADECSQILTSFFKEIRERQKKYASKSR
                     PENSDDI"
     gene            complement(7240..7316)
                     /locus_tag="PL056_03945"
     tRNA            complement(7240..7316)
                     /locus_tag="PL056_03945"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7280..7282),aa:Arg,seq:acg)
     gene            complement(7329..7420)
                     /locus_tag="PL056_03950"
     tRNA            complement(7329..7420)
                     /locus_tag="PL056_03950"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7384..7386),aa:Ser,seq:gct)
     gene            complement(7439..7526)
                     /locus_tag="PL056_03955"
     tRNA            complement(7439..7526)
                     /locus_tag="PL056_03955"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7490..7492),aa:Ser,seq:tga)
     gene            7687..8460
                     /locus_tag="PL056_03960"
     CDS             7687..8460
                     /locus_tag="PL056_03960"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HPM46198.1"
                     /translation="MNQWYQAVVPRDYSIERFFQIEGFLGSMERENVMVLYFEKNDII
                     RKMLEDLDLTVISEGDWVEKWREYFVPVSVGQITVVPPWRRKEGNVVINPSRGFGTGH
                     HETTKISINLIEEVLKKDNCVTSMMDVGTGSGILAISALRMKPGIEVTAIDNDSDALE
                     NAFENVVFNDLVSSVNISREPLFRFEKPCNLVVANIISSVLYFLADDLKRLSSKWLVL
                     SGVLESESDVFMQKMDLNSFELIKKVQENEWVGFVFKRR"
     gene            8462..9364
                     /locus_tag="PL056_03965"
     CDS             8462..9364
                     /locus_tag="PL056_03965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPM46199.1"
                     /translation="MAGKKNEETQKATQIIDLSKREDLDFSGDRFILLEIYGANLGKR
                     RDIDDDSIVIGRGENCNIIVNDPSVSRNHLRIFKKDKLFYAEDLGSTNRTYVNEDEIS
                     GIKKLENGDRVKIGNTIFKFISSHDMEAEYYDQLYQFSIRDGLTGLYNRKSFDDKLES
                     EFSRCKRYGHSMSLVMIDIDHFKPVNDTFGHLAGDAVLTNLSKLFGRFFRSVDFIARY
                     GGEEFVIILPETPLSGAVLTTERIRLAVAQNEVFVNQHTIRVTISAGVAEYKPETASA
                     TALIDAADKKLYEAKNNGRNRVEC"
     gene            9364..9798
                     /locus_tag="PL056_03970"
     CDS             9364..9798
                     /locus_tag="PL056_03970"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HPM46200.1"
                     /translation="MDSFFKKLLSTQFVKFCITGGLGLITDAGIFHLIKTSFGVENWL
                     LLNIIPVVGYSAAVIQNYIINHFWTFRSQTVEMELSKDAFIKFLSVSIISLVPRYIVY
                     NSVLASFTSNRGFVPDIANLSGIIAGTLVNFLGSKFIVFKSK"
     gene            9798..10604
                     /gene="dapF"
                     /locus_tag="PL056_03975"
     CDS             9798..10604
                     /gene="dapF"
                     /locus_tag="PL056_03975"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HPM46201.1"
                     /translation="MMKFFKYQAAGNDFVIVKEPTITSENAENICERHFGVGADGVLI
                     HFNSDSSDAGMKIFNSDGSTAQMCGNGLRCFVSYLVTDYGLGKNPLKIETGRGELEVK
                     WEKLRNGKLSVEANLGKPELKSEETVEFNNKNFDAYSVSMGNPHFVLFPSVKPDASEI
                     HRIANHFQKNSSWNCEVNVEIVTDINKRSKSVFVIVKERGAGFTLSCGTGGGAVMHSL
                     LQKNIVKPDERWKVFFPGGEITYRINDRNEIIMSGVPEKVFEGMLDPAEF"
     gene            10604..11731
                     /locus_tag="PL056_03980"
     CDS             10604..11731
                     /locus_tag="PL056_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46202.1"
                     /translation="MSEFNTNTTDKTLQDYQHSVDRKDSGISKEYRGKPLKLYLEFLV
                     ELSPYLKTGKDIKPYIRDMVNILIEAKRYLDLATIFIFLKNHNEIKSETGNIYPVMKE
                     ILNPHINVFFTKSITYSSVELDEMFSDILGKDHALLMELISFIFKEYGLFNAKLSENI
                     YKKFIEIADEDILSFIEHADSKFLAFYLSSIPQLPFVPAKHVSKWTELILYQSTKEKY
                     TSKIMSAMKEHPSLDILLVFILSPRENERSEMLALMRDCLEMGIVNEELFKTGAVYFL
                     KKSLTSEFYDFNSIPRTQKETICSFILATGGKDLKHIVISMIREKNIHSDPKITETKM
                     VFTNLIKELLPKDPEMTIIVRQMLKDETVEPAVKEAFLKIT"
     gene            complement(11724..12089)
                     /locus_tag="PL056_03985"
     CDS             complement(11724..12089)
                     /locus_tag="PL056_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46203.1"
                     /translation="MNIREKIILTLSFTGLMISTMLVMNEIELRGYNPSLAGVPSSYI
                     FANSFILIILSVFFLKESVKSILFVAGSTLGISVSIYFSATHLLMVNLSPSLYSVSTS
                     YIFTVLFLVCTVAKYLVIK"
     gene            complement(12089..13045)
                     /locus_tag="PL056_03990"
     CDS             complement(12089..13045)
                     /locus_tag="PL056_03990"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HPM46204.1"
                     /translation="MTLSELVLVINFPIFLGWFFKQTKIFREGDISVLRKFVVKVTVP
                     FIIFRNLYHADINEMGQLIPAAAAFFTVSILFTAAVLLIRKFTSSQKEISNSFCIGSF
                     MGNYGYLGWGVLFYFYGESGFTRAVFFTVFFWPVFLLLGFVMVVILSARKLDKESKTA
                     VFSALRSNAVVPLVAVFIALVMNFQNISLAPWLSKSIESFASITIPAILFTVGLSFSI
                     IIKKAHIRAVATGAVLRLFLGMVFGISAMLLTSLLFDIDIVTKKVILLESVMPSAAIS
                     PFFADFIDSDKEAVSGVVTFSTLISLITLPLWYSIIETWSWI"
     gene            complement(13109..14317)
                     /locus_tag="PL056_03995"
     CDS             complement(13109..14317)
                     /locus_tag="PL056_03995"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HPM46205.1"
                     /translation="MNIKHLTFERIVEVSENPEIIRSEEKLHIENCPVCSKRLVAAGM
                     IAPALKKTPEVDHLVDMSAVEMMADNVFDIVSSQENRKGGFSKRAVLALSGVAALIIA
                     VLSVVMSISENDDKKVEDTYAQKEVIQEKSGTDNSEVENVSEFQSSVIDIEKGSAIKR
                     GKYEIAAKVPSRIVHEHENYYSVRKGVVEFKVQSGTDFMVNLNNVALVRVLGTVFTVS
                     VEKTGCSVSVSEGLVEIIDLERGLSRSVAKGGSDTVSKVKRQPVPETVTELSDTIVAE
                     VKKDGSGKISLTDIFSGSHNDEMTRVLINDLETALKFSDSPEIQLNELFGLYRHTGRW
                     GSIIHQWNAKSDRVDTKNNPFLREMNFAACEASIKLYLYDNKVCKRYRQRFPEGPDPD
                     GMEDHLKMAW"
     gene            complement(14314..14889)
                     /locus_tag="PL056_04000"
     CDS             complement(14314..14889)
                     /locus_tag="PL056_04000"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPM46206.1"
                     /translation="MEKQLFCELDDNELVSYAINGSKECWNELVKRYYRMMFATAYRI
                     TGGRDTDDVVQEVLIQLFKSLGSFRKEAAFSTFLYRLTVNIACREVKKMTNLSSIVAV
                     GNEVIERMLEGEPSENSSFESLDKDEMEKMIRKALFKISSDMRMYLTLFYVEELTLKE
                     ISEIVGSPITTVASRIKKGKVLLLKEMERMA"
     gene            complement(14943..15557)
                     /locus_tag="PL056_04005"
     CDS             complement(14943..15557)
                     /locus_tag="PL056_04005"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HPM46207.1"
                     /translation="MMQKYGFIKDIDVKARFDEVKAQLFKEYGIRELHILKQIHSDII
                     LVDEGGTGDGIIITKPLTGALVRTADCFGVALIDRKMNISGIFHSGWRGTELKITVKG
                     VQMMKDLKCSDIEAVIFPGIEKCCFEIGSELVERFKNAGIPVEMKNGKYFADLKTSII
                     EGLKAQGVENIKDMSECTFCGKGFFSFRRDGTEKRHGAFVINFS"
BASE COUNT         4701 a         3455 c         2930 g         4768 t
ORIGIN      
        1 atagaaaaat ccgtctgtat aacttgaatt ttcaaaatca cacgcatctc ctttgataaa
       61 gcaacaattt ttctgtaaat tggtcaatca ctgcaatcct cgaggttaat ataacaaatt
      121 cctgtttccc attcctcgct ctgttccatc agaattgcgc ttaccagtct gaggattgat
      181 tcttcgtttg gaaacaatgt gctcactctg gttcttcttt ttatttctct gttcagtctt
      241 tcaagcaggt tcgtggatct cagtttccgc cgcagcttat gatggagctg gaaaatggcg
      301 aatccttcag ggatattggt atccatccat tcagcaagtt tcggagcttt cttttcgtaa
      361 tgatccactg cttttgcaag ctgccgttgt gcttcatgaa gatctggggc gttgagaatt
      421 ccgcttattg ttcttgaaac ctcatctctc atcgcctgtt tcggaatgta tttgaaagaa
      481 ttcctttgca gatggacatg acagcgggtc cacggaacac cggtaaaaac agctttcatg
      541 gcggttttca gccctggaca gtcgtcactt gttatcattt ttattccgtg cagaccacgc
      601 ttaaccagac tttcaaagaa ctttctccag tgaatttctg cttcacttac tgatgtgctc
      661 actcctataa ccgttctttt tccgtctttt ctaacaccta ccgctataag tgttgcggct
      721 gaaacaactg atccgttcat tcttacattt tcgtatctgg catcaaactg aacatatgga
      781 atctctccaa gaggacggtt cctccatttt tcaagttcct cgtccagaag cttggccgca
      841 cggcttactt ccattgatgt gacttcaact ccgcacagct tttcaaatac tttccgaacc
      901 ttacgggttg taacgccctg aacatacatc tccgccataa ctatttttaa tgctctttca
      961 cttctcaatc ctttttcaag tgctgacgga taaaactcaa tctctccccg gacttgaggt
     1021 atttcaagtt caagtcgccc taaacggctg ttcactgttt tcggtttaaa tccgttggca
     1081 tatcccttcc tgctttctgt ccgctcatac ctttctgccc caagtgcccg acttcgttca
     1141 gctttcatcg cctcattaaa tatcaacgtt aatgcttcct ccagtccttc aaaaccaaat
     1201 tcttcaacaa tctttacagc ttcaattaat ttgctaaaat ctttctgggt catgtctaca
     1261 cctccttttt gtgcaaatcg aaggttgcag atttgaccca cttttgcaag ttcatttcaa
     1321 cctttttttt gaggtcaaaa tgaatttaca gaaacaatgt tacactagca aatatccgtc
     1381 tgggctatcc tcagattcac ttaaaactat tgaaagttca tctgctccaa ataaataaaa
     1441 gaaactgaat aaaattaagc aaataaatcc ttttttcatc aaacacctcc cgtaaggtat
     1501 aaaaaaaata aaaccttgcc tctaagaatc ttttgcagaa ggagtttctt tattttcaat
     1561 atcaatatga gttcctcaac agaaaaatca tatttaaatt ttttataaat ttcaatattt
     1621 ttctaattta aattatatca ttttttatgt aattacaaat ccctttttaa aatcaggatt
     1681 gcttcatatt gtatatgata atcttctctt ctattaaagc ttttataaca ggtggtttac
     1741 aaaaagcttg ttacaaaaga gcatggtgta tattagactg gatcgtccaa aatatgaatt
     1801 ttgtcaatat aactggttcg ctgttttcca ttaagcaatt tctgggttga tttaagtgag
     1861 attaaaagaa atcctaacaa atatcatcgc acacagcgga cattgttggg ttcgctaatc
     1921 ttatcatagt agaaaactct gccaaactcg aaatccacaa ggtatacata gtctttatcc
     1981 ggtacttccg aagaagacca gagaatcttt ttgtctccaa aaatagaata ttcgttggaa
     2041 tcgtcagagt caaaaccaga acatattgac agactataac aatcaatatt tgacaaacaa
     2101 tcatcggtaa ctccgcactc gccccctgtt tcagttgcgg gacagttctg aatgagcgtt
     2161 ctgagttcgc ttattgtagg tatcctgcca ccaatgtttt cacaataggt tacggctgca
     2221 tcccaataca tagaattgct tgaaatatct gaccaattca aactggcata ttcgtaacag
     2281 aacttattac tgtccggaca cttatcctcc ccaccacacg aaaccatcgc aaccgataaa
     2341 aagaaaattg ttgagagaat taaaactctt ttcatatctg actccacaat gaattaagaa
     2401 attcaaatta ttttaactta ttaaaaccga aagacaaata tttttattcc aagatacgga
     2461 gtccagaagc gtttttattt gggatttctt cgctttggtg aatttttgtt ggattttaat
     2521 ggaatcttct acttctcctc acactcacaa atactcacac aaatagaggt cgccataagt
     2581 acatgtgttt atcctttcca actttctcaa gccctcctgt tttcaccatg aggtcaccgt
     2641 tagctgtgta ggtataggtg ttcgtgccat ccatcaattc tcaagctttt tcagcagatg
     2701 caaatcaaca tatccgttta gaatgatctt gttttttacg gctgcttttt caaacacttt
     2761 tttctgtttt tcggtaagtt ctgaattata tttgcattct attaaatact gacttcctga
     2821 ataacggtct gttataaaat cgatctctat tccgtcttcc acataatagg aaggcttctt
     2881 ttctttaatt ttgagataaa tgtaattttc aaacactgcc cctttatctc tgtaaccagt
     2941 ataagcattt cttattccga tgtctcctga atacagtttg ttctgtgaag tgatcaattc
     3001 atttgttttt ccatattttt ttatcagccc gatcaggaaa gtctcttcga aatattcaat
     3061 atacctctta gcattgctcg gagtaatgtt caatattttc gctatgttat ttatgctcaa
     3121 ctgttttccg cttctttcca tcaataacag aaaaaggtct tttattactg atttcatctt
     3181 tatattgtga cgggctatga tatctttgta aataagctgt tcgacaagat tctgaagata
     3241 tgctctgtct ttggtcagaa cgaattccgg cattcctcca tcagacagat attcatcaaa
     3301 atatgtcacc atcagatcct tatccacagc tttcaattta atgtccttaa actcaatcca
     3361 ctcatcaaag tcaagcggtt tgacttcaat tattctgctt cttccggtaa gatatgcatt
     3421 ttcatctata aaaatcgaag aacttgacga actggcgatg atctttacat tttctttgtc
     3481 atatagattt ttcaattgct gctgccattt atctttgtaa gttacttcat caaaaaagag
     3541 atgtattttc tcttccattc ttattttgtg aagaagccgg tattcagcga ttatgtctat
     3601 tattgatttt tcgttgaata gaaaatcatc catgcttacg aataaaatcc ttgtcttgtc
     3661 aaaccctttg cttatcaatt caccgattat cagcttcatt attgatgttt tcccgacacg
     3721 ccggagtcct gcaagaagaa tgatatccct gctttccatg tgttgaaaaa ttctattcag
     3781 atatttatct cttttctgca tctggaataa atatccgtca tcccaccatg gattgaatgt
     3841 gttcaacaac ttctgcatct ttacattccc aaagaataat ttcttggcaa attatacttt
     3901 gccatagtat agcagtcaag aaaaaaaacc actatacaat gttttgggtt tatcaaggat
     3961 ttatgaatga ccctaaatca cagtaactga acagcccgat tttttctttt ttgcttcttg
     4021 tgtgtcggaa tcttcatcgg aatcttcctg aatttcatca atttcgatca tatccggatc
     4081 agagatttct tcaatttctt cagatacttc ttcatcgggt tcttcttcag gacgcttctg
     4141 aatatcagtt ttaaagagtg ctgtgccgtg aacaccgctt ttatcaatat aaataaggaa
     4201 gatcgtctca acattctgat aattatcgaa ttttactgag aaactcttcc cctgtgcaaa
     4261 ctcgatcttg ttccttatcc atacaagtga aaggaaatca tcaccgttct taacttcagc
     4321 acttagcggc tcatcttcaa aaattgatga tggatattcg atctgcggct caacaactgt
     4381 cgcagtggac ggggtaactg taacaaattc aactttcctt gaaaaatcct tcgcgaggaa
     4441 tttatccatt tccgaaccgg cggcaatgct tccctttact gtcaggataa gcgttgtcat
     4501 atcctttgaa acagccgcat ttgatatatg gaacttggga ttttcgatct tatagctttt
     4561 acctgccggc ataaaaaatc tcaaagtcac cggctcctct gtaggcagat aattcatcac
     4621 ttcggcactg actgaatctg cagaaccgtc tccacctgca aagattgact ctgtttcatt
     4681 tccaaaagag ttccatttgt atgtcaccca catatttcct gccggaaccg actgaaaacc
     4741 tttttccgtt tcactgccgg gcatgcagtt tacacccctt tcacaggtat agttgtaaga
     4801 aacatctccc gtaacaccga catcgacttt tctaaatccc cagtaaagct gaccacttgt
     4861 agcagcggtt gttgtcatta tgaattccat gtcatctttt gcatataccc cgcttccatc
     4921 aacaagctcc gagtcttttt cgaacagata cagacgatct ttatgggtgt gtcctgaaaa
     4981 atagatgggt ggtggaaact gccttgtgat cagttccgca agtttgatgc ctgtattaat
     5041 tttctctgtt tcattgaaga tcaggtctgt cggatcactg tcatattcag aggtttcaat
     5101 attccattcc tgagaatcag ttactccaat tacagaatct ttggggaatt tcttcatttt
     5161 atgatatttt ccagttgcat tctgaccaag aggtgtcatg tgactgaaaa gcccgaaaac
     5221 atcatagtca tccatcatta tctcgatcca ggcaagagac ctgtcggtaa gaaatccgcc
     5281 ccagttgctg accggcacag ccgcattatc accaattccg agagcatcgc ctgccgccct
     5341 tctttgcgga gttccgtcat aagtattaag catcagaaat gtctgcccgt taaaattgaa
     5401 cgcataatga agagggccca caaatttcgt ccagtactgc aaaccatccc agtcaagcgg
     5461 tgaaaaaagg ttattctgtt cagtgaactt cgcatacccg tcatggttgc ccggaaccgg
     5521 gaagaacgga atttcaagat tgacaataag tgaaagaata tcttcgtatt ctctctgata
     5581 atttattccg aaaactatgt caccgagcat tgtgacaaaa tggcggtcac ccatattaag
     5641 gttcgatatg gtggttttta tgatcccggc ttgtcttgaa aaatcgagga tcgaatcact
     5701 tttgtaagga tattcccccg aattgtaatt gagatctccc gccatttttg attgcaggtc
     5761 ctctatctgc ggatcagccc agataaaaaa tttcgtggat gcatcaaatt cgggaaaaaa
     5821 cacagaattc cttgatatta ttggcatttc ttcgccttta agtttaacac tgaggtcata
     5881 tcttacactt ggaagaatta caggaacgat cattttcact ttataaagac caaatccgca
     5941 ctgctcaacg gaataaggga taagatcttg aaattcctta acattatcgc ctatttctcg
     6001 gattattctc acatgatcta tttcaggaat attctcagac cggattataa atgacatttc
     6061 gggatcaacc gctgtgacca ttgccggaaa accgattacc ggataaagaa ttctatcaat
     6121 cgtctggttt atgatcatag ttgtctgagt ctggaactga taatccggaa cttcgggctt
     6181 ttcctgcgga tatccgtgaa ttgaaacgaa cgactcactg ttgagagaga agaaaactgt
     6241 catgagaact gccacagcca taaccctgta tcccgttcga aagttaaaaa aattaaccat
     6301 attttttctc cataaacaca caatcaacag aattttaaca attttaaaga tttagtcaat
     6361 gcaaaaagtg gactcgagtg tcatttttct aaaaataaac ttaaaaaatg aaggccggga
     6421 aaactgcaac acacaaacga tccgttaccg ctgcttcctt tcggacctga cgggattcac
     6481 ggggtttgtt gtacagcacc cggccataaa aaaaagatgg cggagagggt gggattcgaa
     6541 cccacggtag gggttaacct acacacaatt tccaatcgtg ctccttcggc ctctcggaca
     6601 cctctccgtt taaaagatcg aaaaaagaat ggcggagaga tagggattcg aaccccaggc
     6661 accccgtaaa gagcgcacct gatttcgagt caggctcctt caaccactcg gacatctctc
     6721 cgcttaaata tcatcagaat tttccggtct tgactttgac gcgtactttt tctgacgctc
     6781 ccttatctct ttaaaaaaag atgttaggat ttgggaacac tcatccgcca gaacgccttc
     6841 gaccacttca actctatgat tcaaccttgt atcctcgaga agattataaa gagatctgac
     6901 agcccctgct ttagggtctc tggggccgaa aacaactctt tttatccttg actggagaat
     6961 tgctccgctg cacataatgc agggttcaag tgttacaaaa agctcgcaat cctcaagccg
     7021 ccagcttccc atgttctctg atgcttccat gatcgcctct atttcagcat gagacaaagg
     7081 cgaattggtc gattctctcc tgttatatcc ttttccgatc actctgtttt caaagaccac
     7141 tacagcaccg acagggactt caccctgtga acctgccgtt tcagcaagtt caagcgcttg
     7201 tttcatataa ctgatttctc tttccatcct gaaagaccat ggcgtgccca ggaggattcg
     7261 aacccccagc ctcctggtcc gtagccagac gctctatcca gttgagctat gggcacactc
     7321 aaaaaaaatg gcggagaggg tgggattcga acccacggta gagttttagc cctacaccca
     7381 cttagcaggc gagctccttc ggcctctcgg acacctctcc gcaaacacaa aaaaataatg
     7441 gcggaggagg taggattcga acccacggac gctctcacgt caacggtttt caagaccgcc
     7501 gccatcgacc actcggccac tcctccgcaa ttttggctca attgttaaaa agagcaaagc
     7561 agaagtataa tgattaaaaa tgactcaaag tcaacatttt ttaaatcact ttccggaata
     7621 aaaactaatt tgcatttttg acagctatcg ttattattaa gtcaattatt tgttttgtgg
     7681 tgaagcatga atcagtggta tcaggcagtc gtaccgagag attattcaat cgaacggttt
     7741 ttccagatcg aaggatttct cggaagcatg gaaagagaga atgtaatggt tctctatttt
     7801 gaaaagaacg atatcatcag gaaaatgctt gaagaccttg atcttacagt tatatctgaa
     7861 ggtgactggg ttgaaaaatg gcgcgaatat ttcgttcctg tatcagtcgg acagatcacc
     7921 gtcgttccgc cgtggcggag aaaagaggga aatgttgtca taaatccttc ccgcggtttc
     7981 ggaactggtc atcacgaaac gacaaagatc tcgatcaatt taatcgaaga ggtgctgaaa
     8041 aaagataact gtgtaacttc aatgatggat gtcgggaccg gctcaggcat acttgcgatc
     8101 tcggcactgc gtatgaagcc gggaattgaa gtgacagcca tcgacaacga ttctgatgca
     8161 cttgaaaatg cttttgaaaa tgtcgttttc aacgatcttg tgagttcagt gaacatcagc
     8221 agggaaccac ttttcagatt tgaaaaacct tgcaaccttg tcgttgcaaa cataatatcg
     8281 tctgttctct attttctggc cgacgacctt aagcggcttt cttcaaaatg gcttgttcta
     8341 tcgggagttc ttgaatctga atctgatgtg tttatgcaga aaatggacct caacagcttt
     8401 gaacttatta aaaaagttca ggaaaatgaa tgggttggat tcgtttttaa gaggagataa
     8461 aatggcggga aaaaagaacg aggaaacaca gaaggcgact cagatcatcg atctttccaa
     8521 gcgggaggat ctcgatttca gcggagacag gtttattctg cttgagatct acggagctaa
     8581 tctcggtaaa agaagagaca tagatgacga ttcgatagtc atcggcagag gcgaaaactg
     8641 caacattata gtcaacgatc cgagtgtaag ccgtaaccac ctgagaatat ttaaaaaaga
     8701 caagctgttc tatgctgagg atctcggaag cactaacagg acatatgtta acgaagatga
     8761 gatctccggc ataaaaaaac ttgagaacgg tgaccgagta aagatcggca acacaatatt
     8821 caaattcatt tcaagccatg atatggaagc ggaatattac gaccagcttt accaattttc
     8881 aatcagggac ggacttaccg gactttacaa cagaaaatct tttgacgaca aacttgaaag
     8941 cgagttttca agatgtaaaa gatacgggca ctcaatgtcg cttgtcatga tcgacataga
     9001 ccatttcaaa ccggtcaacg atactttcgg acaccttgcg ggagatgccg ttcttacaaa
     9061 tctgagcaaa cttttcggaa gatttttcag aagtgtggat ttcatagcca gatacggtgg
     9121 cgaggagttt gtaataatac ttcccgaaac acctttaagc ggagccgttc tcacaaccga
     9181 gaggatccgt ctcgctgtgg cacagaacga agtttttgta aaccagcaca ccatcagggt
     9241 cactatcagt gcaggtgttg ctgaatataa gccggaaaca gcatcagcca cagctctcat
     9301 tgatgcagca gataaaaaac tttatgaagc aaagaacaac ggccgcaaca gggtcgagtg
     9361 ctgatggaca gttttttcaa aaaacttctt tccacacagt ttgtgaaatt ctgcataaca
     9421 ggcggactcg ggctgataac ggacgcagga atatttcatc ttataaaaac atctttcggt
     9481 gtagagaact ggcttctgct caacatcatt ccggttgtcg gatattctgc ggctgtaatt
     9541 cagaattata ttataaacca cttctggaca ttccgttccc agacagtcga aatggaactt
     9601 tcaaaggatg cttttataaa attcctttct gtaagcatta tctcgcttgt tccgagatat
     9661 attgtctata attcagtttt agcttctttt acaagcaacc gcggttttgt tcccgacatt
     9721 gcgaatcttt cagggataat agcaggcaca cttgtgaact ttctcgggag caagttcatt
     9781 gtcttcaaga gcaaatgatg atgaaattct ttaaatatca agcggctgga aatgattttg
     9841 tgatagtcaa agagccgacc attacatctg agaatgcaga aaacatctgc gaaagacatt
     9901 tcggtgtcgg tgctgacgga gttctgatac actttaactc agattcgtcc gatgcgggaa
     9961 tgaaaatatt taacagtgac ggttcaactg cacaaatgtg cggcaacgga ctgagatgtt
    10021 tcgtttctta tcttgtaaca gattacggtt tgggaaaaaa ccctcttaag atcgagactg
    10081 gcagaggaga gcttgaggta aaatgggaaa agctcagaaa cggaaaactt tccgttgaag
    10141 ccaatcttgg aaaacctgaa ctcaaaagcg aagaaacggt tgaattcaac aataaaaatt
    10201 tcgatgcata ttccgtatca atgggaaacc cccacttcgt cctttttcca tctgtaaaac
    10261 cggatgcttc tgaaatacac cgcatcgcaa atcattttca gaagaacagt tcatggaact
    10321 gtgaagtaaa tgtcgagatc gtgacagaca ttaacaagcg gtcaaaaagc gtttttgtga
    10381 tcgttaagga aagaggcgcc ggatttactc tttcctgcgg aacaggaggc ggcgcagtaa
    10441 tgcactcact cttacaaaaa aatatcgtta aaccggatga gcgttggaaa gtttttttcc
    10501 ctggcggaga gataacatac agaattaacg acagaaatga gataattatg agcggtgttc
    10561 ctgaaaaagt ttttgaagga atgcttgacc ctgcggagtt ctgatgagcg aattcaacac
    10621 aaatacgaca gacaaaacgc ttcaggacta ccaacattca gttgacagaa aggattccgg
    10681 catttcaaaa gagtaccggg ggaaaccttt aaaactttat cttgaatttc ttgtcgaact
    10741 ttccccttat ctaaaaacgg gtaaagacat caaaccctat atcagagata tggtgaacat
    10801 tctcatagag gcaaaaagat atctggacct tgcaacgatc ttcatttttc tgaaaaatca
    10861 caatgagatc aaatctgaaa caggaaacat ctatcctgtg atgaaagaga tcctcaatcc
    10921 ccacatcaat gtttttttca caaagtcgat aacttacagc agtgttgagc tggacgaaat
    10981 gttttccgat attctcggca aagaccatgc ccttttaatg gagcttataa gtttcatttt
    11041 caaggaatac ggccttttca atgcaaagct ttctgagaat atctataaga aattcattga
    11101 gatagctgac gaagacatct taagtttcat tgaacacgca gattcaaaat ttcttgcttt
    11161 ctacctctca agcattcctc aacttccttt tgttccggca aagcatgttt caaaatggac
    11221 tgaactgatc ctctaccaga gcacaaagga gaagtacact tcaaaaataa tgtcggcaat
    11281 gaaagagcac ccttcacttg atattctcct tgtattcatt ctttcaccac gcgaaaacga
    11341 acggtctgaa atgcttgcac ttatgagaga ctgtcttgaa atgggaattg tcaatgaaga
    11401 actttttaaa acaggcgccg tatattttct caaaaaatct cttacaagtg aattttatga
    11461 tttcaacagt atcccgagaa cccagaaaga gactatctgt tcgttcatac ttgcgacagg
    11521 gggaaaagat ctcaaacaca tcgtgatctc gatgatacgg gaaaaaaaca ttcacagcga
    11581 tcctaaaata actgaaacaa agatggtttt caccaatctt atcaaagagt tactgccgaa
    11641 agacccggaa atgacaataa tcgtcagaca gatgctcaaa gacgaaacgg tcgagccggc
    11701 tgtaaaagag gcatttttaa agatcactta atcacaagat atttcgctac cgtacataca
    11761 agaaacaaaa cggtgaaaat ataacttgtt gaaacagaat acagtgacgg ggaaaggttc
    11821 accatgagaa gatgcgttgc agaaaaatag atcgagacag aaattccgag cgttgatcct
    11881 gcaacaaaaa ggatggattt tactgattct ttcagaaaga aaaccgataa aatgatcaat
    11941 atgaatgaat tggcaaaaat atagcttgaa ggaacacctg caagtgaagg gttgtatccc
    12001 ctaagttcga tctcgttcat gaccagcatg gttgagatca tcagtcctgt aaatgacaaa
    12061 gtgaggatta tcttttctct aatgttcatc agatccagct ccatgtctca atgatcgaat
    12121 accagagcgg aagagttatc agagatatga gtgttgaaaa tgtaaccact ccgcttaccg
    12181 cctctttatc cgaatctata aaatctgcaa aaaatggcga tatcgccgct gaaggcatga
    12241 ctgattcaag aagtatcacc ttttttgtca cgatatctat gtcaaacagt aacgaagtga
    12301 gtaacattgc ggaaattccg aaaaccattc ctaagaaaag acggagaacg gctcctgttg
    12361 caaccgctct gatgtgagct ttttttatga tgatagaaaa tgaaagtccg actgtgaaaa
    12421 gaattgcagg gatagttatt gaagcaaatg attcaatact ttttgaaagc catggagcaa
    12481 gagagatatt ctgaaaattc attaccagtg ctataaacac tgcaactaaa ggaacaacgg
    12541 cattgcttct caaagctgaa aaaactgctg tctttgattc tttgtccagc tttctggctg
    12601 agaggataac aaccatcaca aatccgagaa gaagaaaaac aggccagaag aacactgtga
    12661 aaaaaacagc tcttgtaaaa ccgctctcac cgtagaaata aaaaagaaca ccccagccga
    12721 gatatccgta gttacccatg aatgagccga tgcagaaact gtttgatatc tctttttgcg
    12781 aagatgtgaa ctttctgatc agcagaaccg ctgccgtaaa aagaatcgat acagtgaaaa
    12841 acgccgcagc agcagggata agctgaccca tttcgttaat atctgcatga taaagattcc
    12901 tgaaaataat gaaagggact gttactttga caacgaattt ccttaaaacg gagatgtccc
    12961 cttccctgaa tatttttgtc tgcttgaaaa accaccctaa gaatatcggg aagtttatta
    13021 cgagaacaag ttctgaaagt gtcatctcac ctcaattaac aaaccgctga aaggataaga
    13081 ttttatgatg atctgtcaaa taatcggatc accaggccat tttgaggtga tcttccattc
    13141 cgtcgggatc gggtccttca ggaaagcgct gacgataccg tttgcagacc ttgttgtcgt
    13201 aaagatataa ttttattgac gcttcgcatg ccgcgaaatt catttccctt aagaaaggat
    13261 tgttttttgt atcaactctg tcagattttg cattccactg gtgtattatt gagccccagc
    13321 gaccggtatg cctgtataat ccgaacagtt cgttaagctg aatttcagga gaatcagaga
    13381 atttcagagc agtttcaagg tcgtttatca atacacgggt catttcatcg ttatgcgatc
    13441 cgctgaaaat gtctgtcaga gagatctttc ctgacccatc tttttttact tctgcaacaa
    13501 tggtatctga taattctgtg acagtttcag gaaccggctg ccgcttcacc tttgagaccg
    13561 tatcactccc cccttttgca acagacctgc tcagacccct ttcaagatct attatttcaa
    13621 caagtccttc gcttacgctg actgaacatc ctgtcttttc gacagaaacc gtaaaaacag
    13681 ttccaagaac cctgaccaat gcaacattgt tcaaattcac catgaaatcg gttccgcttt
    13741 gaactttgaa ttcaacaaca ccttttctga ctgaataata attttcgtgt tcatgtacta
    13801 ttctgcttgg cacttttgcg gcaatttcat attttcccct ttttattgca gacccttttt
    13861 caatgtcaat gaccgagctt tggaattcag aaacattttc aacttcactg ttatctgttc
    13921 ctgatttttc ctgaatcact tctttttgag cgtaagtatc ttcaactttt ttatcgtcat
    13981 tttcagagat tgacatcact actgacagta ctgctattat caatgcggcg actccggata
    14041 aagccagcac cgctcttttt gaaaaaccgc cttttctgtt ctcctgcgaa gaaacaatgt
    14101 caaaaacatt atctgccatc atttccacag cagacatatc aaccaggtga tccacttccg
    14161 gtgtcttttt caaggcaggg gctatcatac ctgcagctac cagtcttttg gaacataccg
    14221 gacagttttc aatgtggagc ttctcttcag atctgataat ttcaggattt tcagatactt
    14281 ccacaattct ttcaaaagtc agatgcttaa tgttcatgcc attctttcca tttctttaag
    14341 taaaagaact ttcccctttt taattcttga tgccactgtg gtaataggcg atcccactat
    14401 ttccgagatc tctttcaaag tgagttcctc gacatagaaa agcgtaaggt acattctcat
    14461 gtcggaagaa attttaaaaa gtgcttttct tatcattttt tccatttcgt ctttatccaa
    14521 actttcaaat gaactgtttt cactgggctc accttcaagc attctctcaa tcacttcgtt
    14581 tccgacagcc acgatcgaag aaaggtttgt catttttttc acttccctgc atgcgatatt
    14641 gaccgtcagg cgatagagaa atgtcgaaaa tgccgcttct ttacggaaag aaccgagtga
    14701 tttaaagagc tggatcagaa cttcctgcac aacatcgtca gtgtcccttc ctccggttat
    14761 tctgtaagct gttgcaaaca tcattctgta ataccttttg accagttcat tccagcactc
    14821 ctttgaaccg tttatagcgt atgaaacgag ttcattgtca tcaagctcgc aaaacaattg
    14881 cttttccaat ctgtttccca aaaaacggtt catgataaat tagtatgttt ccatggatat
    14941 ttttacgaaa aatttataac aaaagcaccg tgcctttttt ctgttccatc ccttctgaaa
    15001 gagaaaaatc ctttgccaca aaatgtgcat tcagacatat ctttaatatt ttcaacacct
    15061 tgtgctttca aaccttctat gatcgaagtt ttaagatcgg caaaatattt gccgtttttc
    15121 atttcaacag gaatgccggc atttttaaat ctttcaacca gttcagagcc gatctcaaaa
    15181 cagcactttt caatcccggg aaatatcacc gcttcgatat ctgaacactt aagatccttc
    15241 atcatctgaa cacccttgac tgttattttg agttcagttc cccgccagcc gctgtgaaat
    15301 attccggaaa tgttcatttt cctgtcaatc agcgcaactc cgaaacagtc ggcagtacgg
    15361 acaagtgcac ctgtcaacgg cttcgtgatt atgatgccat ctcccgttcc cccttcatca
    15421 acaagaataa tgtcgctatg tatctgcttt aaaatgtgga gttcccggat cccgtattcc
    15481 ttgaaaagct gtgccttcac ctcatcaaaa cgggctttga catcaatatc cttaatgaat
    15541 ccgtatttct gcatcatatc aaccaccgta aaagatcatg ctgatacttt acttattttg
    15601 ggcggaaaag aaaatattga gaaaaaagcg gttaagaata aaattaatga gctgtccttt
    15661 tatttatctg agaacggagg gttcccatga agaagatctt tacactggtt tttgtcacac
    15721 tttttgcact cattatcgtt tcctgcggcg aggaatccaa ggaaacaggt gatactggcg
    15781 acacaggtga tactggtgat accggcaata ctggtaacac aggagataca ggaaataccg
    15841 gcaacaccgg ggat
//
LOCUS       DAOOYB010000042        15771 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_3663_length_15771_cov_13.989374, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000042 DAOOYB010000000
VERSION     DAOOYB010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 15771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15771
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3663_length_15771_cov_13.989374"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            65..1630
                     /locus_tag="PL056_04010"
     CDS             65..1630
                     /locus_tag="PL056_04010"
                     /inference="COORDINATES: protein motif:HMM:NF024352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TaqI-like C-terminal specificity
                     domain-containing protein"
                     /protein_id="HPM46208.1"
                     /translation="MDKRKKLVKLFNDIKMFIETNSLKATPHSFLLNRFSLSINNKRP
                     GAVSLSGITKHEFSLINKEFEDRLKRDFVLSLSISSIISLPSRKIFELDNIKVSPRSS
                     ILLLTDTVVIPYEIEKKVSLFIGYGENVFDSAPFQNIEHYPVSRDVPFSISLQSTLFD
                     LISNDTTKLRPFSFDVIFDDAASFSFPSENLIENVSKLLKTKGDYHLTLKKNFLSSPL
                     TKKCKKSLYSFFNANEINRFSSFLHMKLEKRPETNVTTVSNVVIKDHIENRVIKLEKE
                     RLDFNNLLTFDDGIRPEQINIISKIESRAANSSGECFRFFIGMFKEGSSVPVLEHFRK
                     SSRFKPFIRSKEIEPYSVAKVHQYVLPDKDVFFQIPPFENFEREKLILRYLSVKPVFS
                     YDDAGLYFLNDVAAVVPRTDEIDLLFAEGYYNSKVMEYYYRIKFPHHNKFLKKNFNKI
                     PFYLCSRSIQKIIADLVKEIRDINRSVQLKDESEGIIESRRERMGRLDSFLYQLFKLS
                     ADEIRIIEDSLNG"
     gene            complement(1627..2100)
                     /locus_tag="PL056_04015"
     CDS             complement(1627..2100)
                     /locus_tag="PL056_04015"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HPM46209.1"
                     /translation="MKKEPSANRMQTENNAVENIDETQEKETVAGKITVRDIYSGETY
                     PLEKISMHYPVFIEISASWCPACKEMEKTTEKLYEYFKGKVFFIRLFMAGDGSLDENS
                     VIPSMEIASSPEEMKIEYSEALPRVLILSRNGKETVADLTGTYPLLYYYGILSEL"
     gene            complement(2156..3268)
                     /locus_tag="PL056_04020"
     CDS             complement(2156..3268)
                     /locus_tag="PL056_04020"
                     /inference="COORDINATES: protein motif:HMM:NF016584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0007275 - multicellular organism
                     development [Evidence IEA];
                     GO_process: GO:0030178 - negative regulation of Wnt
                     signaling pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dickkopf N-terminal cysteine-rich
                     domain-containing protein"
                     /protein_id="HPM46210.1"
                     /translation="MKKLMFLFAVLLLFSIVSCEEEKKEEPYVPSMAAFFTQYLNTVC
                     DAASKCTSGFVNQSNVLSCPDVIMYNPFPFEGFHKGESVIFKHKYEMMISAETMGWVF
                     ADMDQAQLCFDLIAKMEPCNPLDVQLLDISECASVFSGIKFIKQECFQDEECDNGWCD
                     LKGDRCPGNCVEYKLPDQSCNSTLDRCSPGYVCRSSGCSKAVDGEPGDPCSTDDDCGN
                     FLFCRKTGSDALGNCFKRKSEGEPCLEEKECVVGLSCVDNLCTRSRISDQVGAPCGVQ
                     EEKDENGDPIVLECNAFAKLECGAQKTCQKIAADSNFPCTTRCDKGLYCESNSGTCQW
                     QKTAGLPCVSDENCVSLYCFNTVCATPQCITTQDAE"
     gene            complement(3269..4372)
                     /locus_tag="PL056_04025"
     CDS             complement(3269..4372)
                     /locus_tag="PL056_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46211.1"
                     /translation="MTNIQLILFYLSIFAVLSAFAVFVVEHIRRKIPVYSLKGNFSKD
                     GELLFEALKECHFKNSHEYLQSIKKLAEIYPDEPVFFLFAGDIARRTDPQKALEIHRD
                     VLFRPTTNGKFRSLVLKHIAEDYVALKQNSKALSVLKDSLKSANFPAAHLTMSKIFET
                     EGNFDDAYVQMEKYISGSDIKERAHLKKLAARATYHFFKNNSEASSIKWLEIIAKLSN
                     DPNEIKAVEYSVALIKGKQKKAAVYLKALSEAGENYEILGRSLLIKKEQGHEINSAAE
                     GKFADVFYLLFNPQIKNIETASIISNRTAFYGLLLSRSVSDPEYSGFLKDTISEKTLF
                     VCSECGNDIKNISPVCRNCQKITGRKFRILLED"
     gene            complement(4369..4728)
                     /locus_tag="PL056_04030"
     CDS             complement(4369..4728)
                     /locus_tag="PL056_04030"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HPM46212.1"
                     /translation="MKLARKLTLIMLIAVIAVMISYFAKANDRYVTIDLVFVTIENIQ
                     IWLLSLFCFLAGIIFLIVLVAVDIIMVNYRERKLRKENKNLRDEISRIRNSKLAEIDN
                     GAGSGTNNFDTDPSDSL"
     gene            complement(4801..5430)
                     /locus_tag="PL056_04035"
     CDS             complement(4801..5430)
                     /locus_tag="PL056_04035"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HPM46213.1"
                     /translation="MFTGIIEKTSVVKKIISSGNEYKLTIENPFSNEIKPGDSIAVDG
                     VCLTVEKFSETAIDFFISKVTALKTIAAKYSTGSIVNLERAMNMNGRFDGHIVQGHID
                     TTGVISNIRKIGLGIEMTFRYDRAFSKFIISGGSIAVNGISLTAASVDETSFTVSLIP
                     ETLKRTSFSKQLKTGVEVNLEFDIIGKYVAGLIDNRKGVSNLGSLLEKL"
     gene            5543..7060
                     /locus_tag="PL056_04040"
     CDS             5543..7060
                     /locus_tag="PL056_04040"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrin alpha"
                     /protein_id="HPM46214.1"
                     /translation="MTRITAVILMLTGFIFFSCSNSSTKSGNDSEMNDEATDIATDGN
                     TDDLSDEVLTESEVDTESEETNDSDTVESQQIQTGGIYIFSYMGGNDEKTLFDGKRVL
                     GQGGFFGYSIFNLNEKYWAVGALHESIPPYDFEKATGRMYVFEKGQIPGNIGEAAFVL
                     THPDLLYNAGFSFTAAGTCDINKDGFDDIAVSAHLATYGDAYAAGEIVVFYGTEDGWN
                     EENTSISRVSDGLIQKADSMSQSLVCGDIDGDGFADVLAGGQNAGPELSSGGSQGMVA
                     FFKGSADGLGKNESWTLLPEVEEKAQYFGSSMILEDINGDEIQDLIVSGWGLKESGES
                     DNTGGVYIYHGGTDWQQGPSEKYFGIKDSQFGSVIRIVQVEGRKGLGVIATQEDIFGR
                     IHFFGNMSSPFDVPDTLMSSDSGSFSDFLTVDGIDGYDTLVIAGGKYFFGGTGGLLCA
                     GIGQDYAFKWEKCQWQPGDVSGGFGSSILNIGDINGDKRPEIVVGMPEYIHTVTD"
     gene            7136..7945
                     /locus_tag="PL056_04045"
     CDS             7136..7945
                     /locus_tag="PL056_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46215.1"
                     /translation="MKKLFVMFMMLFIFSFVYAQEEPATDENPPQEVKTDTEVVKENA
                     EEAQKELTTELEPVKEEPAPAEEPERVFTSEKKKIDAKPVYLEEYKPAEKERTKKWSF
                     LPINIAFGTSFQVGFTIAQFTHRNFEIKIGNMMLGPGFRFGSRVKDENNDDGFVPGLT
                     FMGEVAFGKFFGNPLKENCFGLMIGVGSKIIFDIRNIEDDWEEGIYDSDPLYIDFLPL
                     YLTYRWNKGERFYDLSLRIPLVWQNGSYVFTDETRLYNGLPDLTLNFSFVF"
     gene            complement(7923..8537)
                     /locus_tag="PL056_04050"
     CDS             complement(7923..8537)
                     /locus_tag="PL056_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HPM46216.1"
                     /translation="MTTNRFSSSFFNRDALKVASDLLGHFLVRNINGTIVKAMIVETE
                     AYCGAVDKGCHAFGNRRTKRTEAMFLPGGHAYIYLIYGLNYCLNVVTEKKDNPHAVLI
                     RAVEPVEGLDIIKQNRPKIEKTQDLTNGPGKLAKALNIDISLNCCDMVKGNELYLEKN
                     PELPKFSITSATRIGIDYAQGFKDKLWRKYITGNIFVSRKQTKN"
     gene            complement(8571..9145)
                     /gene="rhuM"
                     /locus_tag="PL056_04055"
                     /pseudo
     CDS             complement(8571..9145)
                     /gene="rhuM"
                     /locus_tag="PL056_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991047.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
     gene            complement(9226..10386)
                     /locus_tag="PL056_04060"
     CDS             complement(9226..10386)
                     /locus_tag="PL056_04060"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HPM46217.1"
                     /translation="MKVAIFVIFGFFLFSCDDGWSELSSEELSYTDAYKYCEDLEEDG
                     TSNWRLPRISELRSIITGCDRTEPGGSCKVNDDCLNFYSECMNDPCGCGPKSDGYSKL
                     GDKKLLLSGSWFEDFPDSCGYVDPETYAAAVDFRNAAISFEKEGFVRCLKISKGDERE
                     IDCTEKPLHSQWNLYSKIKQIWDGGKWMPSNISTHNDESSREECRFKCDNTYEWEEKS
                     GTCKSTIEYPWTDPETSLEWSNAEIFRKWEDVEIFCKEMDEGGHTDWRVPDINELRTL
                     ITNCSLTQIDGKCEITLPFSGSESLSEECSGCNPDKSGIYSKLGDSYYLISSDYYELS
                     EGSWENKVFLYIDFLKGRILPDFQPGAESSNSTQISPHCRCVRNVQLSSEII"
     gene            complement(10486..11262)
                     /locus_tag="PL056_04065"
     CDS             complement(10486..11262)
                     /locus_tag="PL056_04065"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HPM46218.1"
                     /translation="MKPIPKIGNFEINSRLFLGSGKYRSNEEMMASVISSGTQVVTAS
                     LRRMDLDKGESLLKPLLENKNIIILPNTAGARTAEEAVFYAELAEAAGLPKWVKLELT
                     PEPRYLLPDPVETLKATEILVKKGFTVLPYINADPMLALRLQDAGAATVMPLASPIGT
                     NKGIIMFEMIRIIIEQARVPVVVDAGIGTPSDAAKAIEMGADFVLVNTAIATAKDPEK
                     MGLAFKLAVEAATIAISAGIPRKYDSAQQSSPELDFIDKL"
     gene            complement(11275..12189)
                     /gene="nadA"
                     /locus_tag="PL056_04070"
     CDS             complement(11275..12189)
                     /gene="nadA"
                     /locus_tag="PL056_04070"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HPM46219.1"
                     /translation="MKEMIEKINFLKKEQNAIILAHTYTHPEVQDIADFVGDSYGLSK
                     KAADAGDFDTIVFAGVRFMAETAKILSPQRKVLLPVLAAGCPMADMIDEHQLAKFKSE
                     NPGAKVVCYVNSDVRTKALSDVCVTSSNAVKIVKKLDAEKILFVPDMHLGSYVAEQVP
                     EKEIICWKGFCPVHHAVKPEDVIEAKKLWPNAVVLMHPECPPQTRITADFILSTGQME
                     TLVKEKKYKQYIIVTESGITYPLSKADKDAEFIELPSASLMCVNMKKIMLTDVLRSLE
                     ENVYEIEIPKAIEEKARAALLKMLELAK"
     gene            complement(12186..12425)
                     /locus_tag="PL056_04075"
     CDS             complement(12186..12425)
                     /locus_tag="PL056_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HPM46220.1"
                     /translation="MSEMNWKEKVKEVLDQVRPALQRDGGDVELIDVADDGIVTVKLQ
                     GACAGCPMSQMTLKMGIEQHIKRQIPEVKEVRNLK"
     assembly_gap    12552..12651
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12652..13623
                     /locus_tag="PL056_04080"
     CDS             <12652..13623
                     /locus_tag="PL056_04080"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPM46221.1"
                     /translation="MAELLRSGVTTFSDMYFFEEDVASAAKEINIRAMIGEGVINFPS
                     PAGRTPEQTIEYTEHLAKKYKDDELISVCCAPHSPYLTHESYLLKLKEISDTYKIPFH
                     IHMAESRFEVENYKASTGKTEFERFEELGLLSERFLAAHCVNLADIELEIMSRNKVTV
                     AHCPSSNMKLGSGIAKVAKMVEMGINVAVATDGSASNNNLNLLAEAELASKLQKVDRR
                     DPTVFKSIDALKSVTSNPGRIAGRNLGTIKTGAPADLVLIKTDSYEMSPVYDPASALL
                     YASSHSDIQAVFVAGKKVVENGHILTIDEENLKKEFSKFVQKVKEEI"
     gene            13765..15108
                     /locus_tag="PL056_04085"
     CDS             13765..15108
                     /locus_tag="PL056_04085"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM46222.1"
                     /translation="MLFDIIRRSIIDFHNGKRKQIVKRNMTFRDIEGKASVFIGVRRC
                     GKSTLLFQLIDDLVSKKVDMKNILYINFFDDRFTELKTKGLSSVIEAYYSLYPEKKGV
                     ETVYFLFDEIQEIPEWEPFIDRILRTEKCRVFITGSSAKMLSKEIATAMRGRSLSFEV
                     FPFSFIEFLKFKEHENLVMTTKNRLILQNDFEEYFIKGGFPEVRNSVESDRIDIHQEY
                     FRTIMWRDVIERHNLSNPQTVIQLGYRLMTSCASLYSLNRLTEYLKSLGFKTSKEFVF
                     TVLSAFEDCYFLFSVPIYDKSEIKRNVNPKKIYCVDHSMMSSIDPQINEKKGQLLENL
                     VFSTIRRSSSAIYYYKTASGKEVDFCWIDRSGKKNLVQVSFDLNDEKTRKREVSALFE
                     AMGEINEKSATLVTMNESGVLELEDRTIQIVPAWEYLINFEYSEKSVFQINDLML"
     gene            complement(15121..>15771)
                     /locus_tag="PL056_04090"
     CDS             complement(15121..>15771)
                     /locus_tag="PL056_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HPM46223.1"
                     /translation="QSSFIYLKDVFDSVILKDVIKRNKIRDIDLLERVIQFVVANVGN
                     TFSATSISRFLKSENRTVAPETILNHIKVCEEAFLFHKVLREDIIGKKILQVNEKYFI
                     TDHGIREAIYGNNERDINLVLENIVFLEMLRRGYKINIGRVHNEEIDFVCRKNNETIY
                     IQVAYLLATEETIRREFDVLKKVPDNYPKFVLSMDQFDFSRDGIKHFNIPDFLTNQ"
BASE COUNT         4615 a         3054 c         3049 g         4953 t
ORIGIN      
        1 gatttacctt gatttttaaa atccgcaacc tataatttat cgaactttga aaagggtcga
       61 aaatatggat aaaagaaaaa agctcgtcaa actcttcaat gatatcaaaa tgttcattga
      121 aaccaactct cttaaggcaa caccgcattc ttttctgtta aaccggttct ctctttcaat
      181 aaacaataaa agacccggtg cagtatcgct gtcaggcatt acaaaacatg aattttctct
      241 tatcaacaaa gagtttgaag accggctgaa aagggatttt gttctctctc tgtctatttc
      301 ttcaataatt tcacttcctt caagaaagat ctttgaactt gacaatataa aagtgtcacc
      361 gagaagttca atactgcttt taacggatac ggttgttatt ccgtatgaaa ttgaaaaaaa
      421 ggtgtctctg tttatcggtt acggtgaaaa tgtctttgat tcagctcctt tccagaacat
      481 tgaacattat ccggtttcaa gagatgttcc tttctcaatt tctttacagt caacactttt
      541 tgatcttata agcaacgata caaccaagct tagaccgttt tcttttgatg tcatatttga
      601 tgatgctgca agtttcagtt ttccaagtga aaatctaata gaaaatgttt caaaactctt
      661 aaagacaaaa ggtgattatc atctgactct taaaaagaac tttctttcaa gtcctctgac
      721 aaaaaaatgc aaaaagtcat tgtattcttt ctttaatgca aatgaaataa acagattttc
      781 ctcctttctt cacatgaagc ttgagaaaag acccgagact aatgtcacaa cagtttcaaa
      841 tgttgtaatt aaagaccata ttgaaaacag ggttatcaag ctggaaaaag aacggcttga
      901 ttttaacaat ctcctcactt ttgatgacgg gatccggcct gaacagatca atatcatttc
      961 aaaaattgaa tccagagcgg caaacagttc aggagagtgt ttcaggtttt ttatcggtat
     1021 gttcaaagag ggcagcagtg ttcccgttct tgagcatttc agaaaaagca gcagattcaa
     1081 accttttata aggtcaaagg aaatagaacc ttattccgtt gcaaaggttc accagtatgt
     1141 tcttccggat aaagatgttt tttttcagat acctcctttt gaaaattttg aaagggaaaa
     1201 gctcatactc cggtatcttt ctgtaaagcc cgtcttttca tacgatgatg caggtcttta
     1261 ttttcttaat gatgttgcgg cagttgtacc cagaactgac gaaatagatc ttctttttgc
     1321 tgaaggttac tataattcaa aggtaatgga atactattac agaataaaat ttcctcatca
     1381 caacaagttt ctgaaaaaga attttaacaa aatacctttt tatctctgca gccgcagcat
     1441 tcaaaagata atagccgatc ttgtaaagga gatcagggat attaaccgtt cagtccagtt
     1501 gaaagacgaa agcgagggaa ttattgagtc aagaagagaa agaatgggcc ggctggacag
     1561 ctttctttat cagctcttta agctttcagc agatgagatc agaataatag aggattcttt
     1621 aaacggctaa agttcagaaa ggattccata ataatagagc aatggatatg ttcctgtcag
     1681 gtcagcaacg gtttcttttc cgtttctgct taatattaaa accctcggga gtgcttcgct
     1741 gtattcgatc ttcatttctt ctggagacga tgctatttcc atcgaaggta taacgctgtt
     1801 ctcatcaaga gatccgtctc ccgccataaa aagacggatg aaaaacactt tccctttgaa
     1861 atattcataa agtttttcag tggttttttc catttctttg caggcaggac accatgaagc
     1921 cgatatttca ataaaaacag gataatgcat tgaaattttt tctaaaggat atgtttcacc
     1981 gctgtaaatg tcccttacag ttattttgcc ggcaacggtt tctttttcct gagtttcgtc
     2041 aatgttttca acagcattgt tttcagtttg catcctgttt gcagatggtt cctttttcat
     2101 tcctgagcag gatgctgata ctgaaaataa tatcagtatg aaaaaaatgt gaaaattact
     2161 ccgcatcttg agttgtgata cattgaggag ttgcgcagac tgtgttgaag cagtaaagtg
     2221 aaacacaatt ttcatcagaa acacacggaa gaccggctgt tttctgccat tggcatgtgc
     2281 ctgaatttga ttcacagtac aatcccttgt cacatcttgt cgtacatgga aaatttgaat
     2341 ctgctgctat tttctgacat gttttctggg caccgcactc aagttttgca aatgcgttgc
     2401 attcaaggac gataggatcc ccgttttcgt ctttttcttc ctgaactccg caaggggctc
     2461 cgacctgatc gcttattctt gatcttgtgc agagattgtc cacacagctt aatccgacaa
     2521 cacattcctt ttcttccaga cacggttctc cttcactttt tcttttaaaa cagtttccca
     2581 gagcatctga ccctgttttt ctgcagaaaa gaaaattacc gcagtcatca tcggtggaac
     2641 agggatctcc aggttctccg tcaaccgcct ttgaacagcc ggatgagcgg cagacatatc
     2701 ccggagaaca tctgtccaga gttgaattac atgactgatc cgggagtttg tattcaacac
     2761 agttgcctgg acagcggtca cctttcaggt cacaccatcc gttgtcacat tcttcatcct
     2821 ggaagcattc ctgctttatg aattttatac cgctgaaaac cgaggcgcat tcacttatgt
     2881 caagaagttg aacatcaaga gggttgcagg gctccatttt tgcaataagg tcaaagcata
     2941 gttgtgcctg atccatgtct gcaaaaaccc aacccattgt ttcagcactt atcatcattt
     3001 catatttgtg tttaaaaata acactttccc ctttgtgaaa tccttcaaat ggaaatggat
     3061 tgtacattat aacatcaggg cacgacagaa cattgctctg atttacaaag ccggatgtgc
     3121 attttgaagc tgcatcacag acagtgttaa gatactgtgt aaagaacgcc gccatgctcg
     3181 gaacataagg ttcctctttt ttctcttcct cgcatgaaac tattgaaaat aaaagaagaa
     3241 cagcaaacag aaacatgagt tttttcattt agtcctccaa aagtattcta aatttccgcc
     3301 ctgttatttt ctgacagttt ctgcataccg gtgatatgtt ttttatatca tttccgcatt
     3361 cagaacatac aaaaagtgtt ttttccgata tagtgtcttt aagaaaaccg ctgtattccg
     3421 gatcgcttac cgaacgggag agaagaagtc cgtaaaatgc agttctgttg ctgatgattg
     3481 aagccgtttc gatgtttttt atctgagggt tgaaaaggag atagaacaca tctgcaaatt
     3541 ttccttcggc agcggaattt atttcgtgcc cctgttcttt tttgataaga agcgatcttc
     3601 cgagaatttc gtaattttcg ccggcttcag aaagggcttt taagtatact gccgcttttt
     3661 tctgttttcc ctttataagt gcgacagagt attcaacggc ttttatctca tttggatcat
     3721 tgcttagttt ggcaattatt tcaagccatt ttatcgaaga tgcttccgaa ttgttcttga
     3781 agaaatgata tgtcgctctt gcggcaagtt tttttaaatg tgccctttcc tttatatcag
     3841 atcccgaaat atatttttcc atttgaacat acgcatcatc aaaatttcct tctgtttcaa
     3901 atattttgga cattgtcaaa tgtgccgcag gaaaatttgc actttttaat gaatctttca
     3961 gaaccgaaag ggcttttgaa ttctgtttca atgcaacata atcttcagca atgtgtttta
     4021 agacaagaga tctgaacttt ccgtttgtag tcggtctgaa aagaacatca cggtgaattt
     4081 caagagcttt ctggggatca gttcttcttg ctatatcacc tgcaaaaagg aaaaaaaccg
     4141 gttcatcggg ataaatctct gcaagttttt ttatgctttg aagatattcg tgactgttct
     4201 taaagtggca ttccttgagc gcttcaaaaa gaagttctcc gtcttttgaa aaatttccct
     4261 tgagcgaata gaccggtatc tttcttctga tatgttcaac aacaaataca gcaaaagccg
     4321 ataaaacagc gaatatagat aaatagaata atatcagctg gatatttgtc ataaactgtc
     4381 gcttggatcg gtgtcaaaat tatttgttcc tgagcctgca ccgttatcga tctcagcaag
     4441 cttgctgttt ctgatccttg agatctcatc tcttaagttc ttgttttcct tgcgtaattt
     4501 tctttccctg tagttgacca taattatatc gacagcaaca agtactatca aaaagattat
     4561 tccggcaaga aaacagaaaa gggaaaggag ccagatctga atgttttcaa tcgttacaaa
     4621 gacaaggtca attgtgacat atcggtcgtt cgcttttgca aaatacgata tcattaccgc
     4681 aattactgca ataagcatta taagcgtcag ttttctggca agtttcatat ttatctcctt
     4741 aaagaattaa gcgcagttat tatacttaaa acaggcatca tttaaaattt ttcctaaaaa
     4801 tcagagtttt tcaagcagag atccgagatt tgacacccct tttctgttgt ctattagccc
     4861 ggcgacatat ttgcctatga tgtcaaattc cagattaact tccactcctg ttttcagctg
     4921 ttttgaaaag gatgttcttt tcagtgtttc gggaatgaga gagacggtga atgatgtttc
     4981 gtcgactgat gccgcagtaa gcgaaatccc attcactgca atcgatccgc ctgaaatgat
     5041 gaattttgag aaagccctgt catatctgaa tgtcatctct attccgagac caatttttct
     5101 gatgtttgaa atcactccgg ttgtgtcaat gtgtccctga acaatgtgtc cgtcaaaccg
     5161 tccgttcata ttcattgccc gttcaaggtt tacaattgag ccggttgaat attttgccgc
     5221 aattgttttc aaagctgtaa cttttgaaat aaaaaaatca attgcagttt cagaaaattt
     5281 ttcaactgtc agacacactc cgtcaactgc gatcgaatca ccgggtttta tctcatttga
     5341 aaagggattt tcaattgtca gcttatattc atttcctgaa ctgatgattt tctttacgac
     5401 cgatgtcttt tcaattattc cggtaaacat ctcttacctc acaagtagtt cagcaaatca
     5461 taaatgaaat ctgttttttt gcaacaatca cttattagat gtataatatc tgccggatca
     5521 aatttgttca caggagaata tgatgacaag aattacagca gttattctga tgttgaccgg
     5581 gtttatattt ttcagttgca gtaacagcag cacaaaatcg ggaaacgatt cggaaatgaa
     5641 tgatgaagca accgatattg ccaccgacgg gaacaccgac gacttgtccg acgaagtttt
     5701 aacggagtcg gaagttgaca cggagtcgga agaaacaaat gactctgaca cagttgagtc
     5761 tcagcagatc cagaccgggg gaatttatat cttctcttat atgggcggca atgatgaaaa
     5821 aacgcttttt gacggaaaaa gagttcttgg gcaaggcggc ttttttggtt attcaatatt
     5881 caatctgaat gaaaaatatt gggcagtcgg tgctcttcat gaatctattc cgccgtatga
     5941 ttttgaaaag gcaactggca gaatgtatgt ttttgaaaaa gggcagatac ctggaaatat
     6001 tggtgaagcc gcatttgttc tgacacatcc cgatctcctt tacaacgccg gtttttcatt
     6061 tacagctgcc ggaacctgcg acataaataa agacggtttt gatgatatag ctgtttcggc
     6121 ccatctggca acttacggag atgcttatgc cgcaggggaa attgttgttt tttatggaac
     6181 ggaagatggc tggaatgaag agaacacttc aatctcaaga gtttcagacg gtcttattca
     6241 gaaagctgat tcaatgagtc agtcacttgt ttgcggagac attgacggag acggatttgc
     6301 cgatgttctt gcagggggac agaacgctgg accagaactt tcaagtggcg gaagtcaggg
     6361 gatggttgca ttttttaagg gaagtgcaga cggacttgga aaaaacgagt cgtggacact
     6421 gctgccggaa gttgaagaaa aagcgcagta ttttggaagc agtatgatac ttgaagatat
     6481 aaacggagat gaaattcagg atcttatcgt ttcgggctgg ggacttaagg aaagtggcga
     6541 atctgacaac acagggggag tttatatata tcacggcgga actgactggc agcagggacc
     6601 gtcagaaaaa tatttcggaa taaaagattc acagttcggt tcagtcataa gaattgttca
     6661 agttgagggt agaaaaggac tgggagttat tgcaacacag gaagatattt tcggcagaat
     6721 acattttttc ggaaacatga gttctccttt tgatgttcct gatacactta tgtcttctga
     6781 cagcggttcg ttctcagatt ttctgaccgt tgacggaatt gacggatatg acacactcgt
     6841 aattgcaggt ggaaaatatt tttttggcgg gacaggagga cttctttgtg ccggtatagg
     6901 acaggattac gcttttaaat gggaaaaatg tcagtggcag cctggagatg tgtcgggtgg
     6961 attcgggtca agtatcttaa atatcgggga catcaacggt gacaaaaggc cagaaattgt
     7021 cgtcggaatg ccagaatata ttcacacagt taccgattaa actgctgaaa atcaataatt
     7081 tgacccggaa aatgttacaa aatcaacgct tttaaatgtt tttttggagg gtgatatgaa
     7141 aaagcttttt gttatgttta tgatgctgtt tattttcagt tttgtttatg ctcaggaaga
     7201 gccggcaact gatgaaaacc cgccgcaaga ggttaagaca gatactgaag ttgtaaagga
     7261 aaatgctgaa gaggcgcaaa aagagctgac aactgaattg gaacctgtaa aagaggagcc
     7321 cgctcccgca gaagaaccgg aaagggtttt tacaagtgaa aaaaagaaaa ttgatgccaa
     7381 acccgtttat ctggaagagt ataaacccgc ggaaaaggaa agaactaaaa aatggtcttt
     7441 tcttccgata aatatcgctt tcggaacttc atttcaggtc ggtttcacaa tcgcgcagtt
     7501 cacgcacagg aattttgaga tcaagatcgg aaacatgatg ctcggtcccg gtttcagatt
     7561 cggctcaagg gtgaaagacg aaaacaacga tgacggtttt gtcccagggc tcacatttat
     7621 gggtgaagtg gcttttggaa aatttttcgg aaatccgctg aaagagaact gtttcggttt
     7681 aatgatagga gtcggatcaa aaataatctt tgacattaga aacattgaag atgactggga
     7741 agaaggaatt tatgacagtg atcctctgta catagatttt cttccattgt acctgactta
     7801 ccgctggaat aagggtgaaa gattctatga cctttcgttg agaattccgc ttgtctggca
     7861 gaacggatct tatgttttca cagatgaaac cagactttat aacggtcttc ctgacctgac
     7921 ccttaatttt tcgtttgttt tctagatacg aaaatatttc cggttatata tttccgccac
     7981 agcttatctt tgaatccttg agcataatca atccctattc ttgtcgcaga agttattgaa
     8041 aattttggca attctggatt tttctcaaga taaagctcat ttcctttcac catatcgcag
     8101 cagtttaacg aaatgtcaat gttcagtgct tttgcaagtt tccccggacc gtttgtaagg
     8161 tcttgtgtct tttcaatttt aggacggttc tgcttaataa tatcaagtcc ctcgaccggt
     8221 tcgaccgctc gtatcagtac tgcatgagga ttgtcctttt tttcagtcac cacattaaga
     8281 cagtaattca gcccgtaaat gagataaata tatgcatgac ctcccggaag gaacatcgct
     8341 tcagtccgct ttgtccgcct gttcccaaaa gcgtgacaac ccttgtcaac cgctccgcag
     8401 taggcctctg tctcaactat cattgctttg acaattgttc cgttaatatt cctgacaaga
     8461 aaatgcccaa gcaggtcact agccactttc aaagcatccc ggttaaaaaa acttgaagaa
     8521 aaacggttag ttgtcatgat gatttgtggg aatacataag gataatcatg ttatttcaca
     8581 gataattttt taatgtcatt ttcaagctct tgaaggcttt cttctctatc ttcttttctt
     8641 ttttcgttta agtatttttc atattcctgc ccagcttttt caatagctga ttcatgagaa
     8701 atttttcctg catgtgtcaa aagttccttt ccattcattt taattattac atccagtcgc
     8761 tcgacccagt ctttcatata cattggaatc tgttgttgag ccattgattc agcaaaagca
     8821 atcgaaattg agtgccctgt ttggatttga ctctgtatcc tactgaaatt attacatcaa
     8881 gattgtaaaa gttcggagct ttctgctgaa attcggaatt tccgaatttc ttcagaactt
     8941 cttgttcaac aagctcgcct tcagcaaaaa tattttttat gtgttcatta attgttgact
     9001 ttgcttttcc aaaaagttca gccatgtgat cttgtgtcag ccagacagtt tcatcttcga
     9061 cacgaacatc aatctttatt tttccgtcag ctgcttcata aattataatc tgcgaatcgc
     9121 taaactttgt gtggattgat tccatttttc tctccgttcg ctcgtaacct gaccagagag
     9181 attttatcgt ttataatcca tttatcaaaa taaatcagag gaaacttaaa ttatttctga
     9241 agataactgg acattcctca cacagcggca gtgtggtgaa atttgcgttg aattagaact
     9301 ctctgccccc ggttgaaaat cgggcagaat gcggccttta agaaaatcta tataaagaaa
     9361 cactttattt tcccatgatc cttcagaaag ttcataataa tctgaactga taagataata
     9421 tgaatctccg agttttgaat aaatgccgct cttgtcagga ttacatccgg aacattcttc
     9481 acttaaactt tcactgcctg aaaagggcaa agttatctca catttcccgt cgatttgtgt
     9541 caaagagcag tttgttataa gcgttctaag ctcattaatg tcaggcactc tccagtctgt
     9601 atgccctcct tcgtccattt ccttgcagaa aatttcgaca tcttcccact ttctaaatat
     9661 ttctgcattt gaccattcca gtgatgtttc cggatcagtc cacggatatt caattgtgct
     9721 tttgcatgtt cctgattttt cttcccactc ataggtattg tcacacttaa acctgcattc
     9781 ttcacggctc gattcatcat tgtgcgttga aatatttgac ggcatccatt tgccgccatc
     9841 ccatatctgt ttgatttttg aatacaagtt ccattgagaa tgcagcggct tttcagtgca
     9901 gtcaatctcc ctttcatctc ctttgctgat tttcagacat cttacgaaac cttctttttc
     9961 aaaggaaatg gccgcattac ggaaatcaac tgcggcggca tatgtttcag gatccacata
    10021 gccgcaggaa tcgggaaaat cctcaaacca gctgcctgac aaaagtagtt ttttgtctcc
    10081 gagttttgaa taaccatcag attttggacc gcatccacag ggatcgttca tacattcact
    10141 gtagaaattc aaacaatcat cgttgacctt gcaacttcca ccaggttcgg ttctgtcaca
    10201 tccggttata atagatctca actcggatat tctgggtagc cgccaattag aggttccgtc
    10261 ttcttcaaga tcttcacagt atttataggc atccgtgtag ctcaattcct cgctgctcaa
    10321 ttctgaccag ccgtcatcac aagaaaaaag aaaaaaacca aagatgacaa aaattgcaac
    10381 tttcatcaga accaccctta aataactgaa aaaacgaatc ttttgttaca aaacattatt
    10441 cattttatta aagaaaatgc aaatgcacat tagaaaaatt aaaaattaca gcttatcaat
    10501 aaagtcgagt tcggggcttg actgctgtgc tgagtcatat tttctgggga ttccggctga
    10561 tattgcgata gttgcggctt cgactgcaag tttgaatgca agacccattt tttcggggtc
    10621 tttggctgtg gcgatcgctg tgttcacaag gacaaaatca gctcccattt cgattgcttt
    10681 tgcagcatct gagggagttc ctatccctgc gtcaactaca acgggaactc ttgcctgttc
    10741 gattatgatc cggatcattt caaacatgat tatccctttg ttggttccaa tcggtgaagc
    10801 aagcggcata actgttgcgg ctcctgcatc ctgaagacgg agtgcaagca tcgggtcggc
    10861 gttgatatat ggaaggacag taaaaccttt tttcacaagg atttcagtgg ctttcagtgt
    10921 ttcaacgggg tctggaagaa gatatctcgg ttccggtgtg agctcaagtt tgacccattt
    10981 gggaagtcct gccgcttcag caagttctgc ataaaatact gcttcttctg ctgttctcgc
    11041 tcctgcggtg tttggcagaa tgatgatatt tttattttca agcagcggct ttaaaagcga
    11101 ttccccttta tcaaggtcca ttctgcgtaa agaagctgtc acgacttgtg ttccggaact
    11161 tatgacagaa gccatcattt cttcgtttga acggtacttt ccgcttccaa gaaaaaggcg
    11221 tgaatttatt tcaaaatttc cgatcttagg gattggtttc atgattttct ccggttattt
    11281 tgcaagttca agcattttta aaagtgccgc tcttgctttt tcttcgattg ctttaggtat
    11341 ttcgatttca taaacatttt cttcaagcga tctcaaaaca tctgtaagca ttatcttttt
    11401 catgtttaca cacataagcg atgcactcgg aagttcaata aattccgcat ctttatctgc
    11461 tttgcttaat ggatatgtaa ttcctgattc agttacaatg atatactgct tatatttttt
    11521 ttccttcacg agtgtttcca tctgaccggt cgaaaggata aaatctgctg taattctagt
    11581 ttgaggcgga cattcaggat gcatcagaac tactgcgttt ggccagagct tttttgcttc
    11641 aatgacatct tcgggtttta ctgcatgatg aaccgggcag aaccctttcc agcagatgat
    11701 ctctttttcg ggaacctgct cagccacata acttccaaga tgcatgtcgg gaacaaaaag
    11761 aatcttttct gcatcaagtt tctttactat tttcacagcg ttagagcttg taacacacac
    11821 atcgctcagt gcttttgtcc tcacatcgct gttgacatag cacaccactt tggctccagg
    11881 attttcgctt ttaaactttg caagctgatg ttcatcgatc atgtcagcca tcgggcagcc
    11941 ggctgcaaga accggcagaa gaactttcct ttgaggagaa agaattttcg cagtttcagc
    12001 catgaatctc acacctgcaa aaactattgt gtcaaaatca cccgcatctg ccgctttttt
    12061 tgaaagtccg taactgtcac cgacaaaatc ggcaatgtcc tgaacctcag gatgagtgta
    12121 agtatgtgca agtattatag cattctgctc ttttttaaga aaatttatct tttcgatcat
    12181 ttctttcatt tcaggtttct tacctctttt acttccggga tctgcctttt tatgtgctgt
    12241 tctattccca ttttgagtgt catctggctc atcggacagc cggcacatgc accctgaagt
    12301 tttaccgtaa cgattccgtc atcagcaaca tcgataagct cgacatctcc accgtctctt
    12361 tgcaatgcgg gacgcacctg atcaagaact tccttaacct tttccttcca gttcatttcg
    12421 ctcatttttc tctcctattg agatggttgt tcaaaacttt aaaaaaacaa gttcagacaa
    12481 ttatctaatt ataacaactc atgtcaagga agaaatgaat ccttgtttta atggatgagt
    12541 ttttgtatta tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn natggctgaa
    12661 cttttaagaa gcggtgttac aacattttca gacatgtact tttttgaaga ggatgtggca
    12721 agtgccgcca aagaaataaa catcagggcg atgatcggtg aaggggtgat aaattttcca
    12781 tctccggcag gtcgcactcc tgagcagacc attgaatata cggaacatct tgcaaagaaa
    12841 tataaagatg atgaactgat aagtgtgtgc tgcgctcctc attcgcctta tctgacacac
    12901 gaatcgtatt tgcttaaact caaagaaata tcagatactt acaaaattcc attccatatc
    12961 cacatggctg aaagccgctt tgaagttgaa aattataaag cgtcaaccgg caaaaccgaa
    13021 tttgaaagat ttgaagaact tggattgcta agtgaaagat ttctggcggc tcactgtgtc
    13081 aatcttgctg acattgaact tgaaattatg tcccgaaata aagtgaccgt cgcacattgt
    13141 ccctcaagca acatgaagct tggaagcggt atcgcaaaag tagccaaaat ggttgaaatg
    13201 gggataaatg tcgcagttgc aacagatggc agtgcaagca ataacaactt gaatttattg
    13261 gcagaagctg aacttgcctc aaagctccag aaagttgata gacgcgaccc cacagtgttt
    13321 aagtcaattg atgcactcaa atctgtcaca agtaacccgg gaagaattgc aggcagaaac
    13381 ttaggcacaa taaaaaccgg agcccccgca gaccttgtat taataaaaac agacagttac
    13441 gaaatgtcac cggtttatga ccccgcatcg gcacttcttt acgcatcaag ccattctgac
    13501 attcaagcag tatttgtagc aggaaaaaaa gttgtcgaaa atggacacat cctcaccatt
    13561 gatgaagaga atctaaaaaa agagttctca aaattcgttc aaaaagtaaa agaagagata
    13621 taagaactgt tgtaaaaaaa caaaaaagca tcaattgaga cagacttctg gagaatttgc
    13681 ttgccaattt tgcttaaatg tggtagcgta attcgccaat ttgtgtgaaa agtggcagct
    13741 aaattatccg gaatggaggg caaaatgctt tttgacatta tcagacgatc aattattgat
    13801 ttccataatg gtaaaagaaa acaaatagtt aagagaaata tgacatttcg tgatattgaa
    13861 ggcaaggctt cagtttttat tggtgtgagg cgttgtggca agtcaactct tcttttccaa
    13921 ttgatagatg atctcgtttc taaaaaagtt gatatgaaaa acattttgta cattaatttt
    13981 tttgatgaca ggtttacaga gctcaaaaca aaagggcttt catcagtgat tgaagcatat
    14041 tattcacttt atcctgaaaa aaaaggtgtt gaaactgtct attttctttt tgatgaaatt
    14101 caggaaatac ctgaatggga gccatttata gacagaatat tgagaactga aaaatgcaga
    14161 gtttttataa cgggttcttc cgcgaaaatg ctttcaaaag aaattgcaac agcaatgagg
    14221 ggcagatctt taagttttga ggtgttcccg ttttcattta ttgagttttt aaagtttaaa
    14281 gagcatgaaa accttgttat gacaacaaaa aaccgattga tattgcaaaa tgattttgaa
    14341 gaatatttta tcaagggtgg ctttcctgag gttagaaatt cagttgaatc cgacagaata
    14401 gacatacatc aggaatattt tagaacaata atgtggcgag atgtaattga gcggcacaac
    14461 ctttcaaatc cgcagactgt aattcagctt ggttatcgtt tgatgacaag ctgtgcatcg
    14521 ctatattcac ttaaccggct cacagaatat cttaaatcat taggatttaa aaccagcaag
    14581 gagtttgttt ttacagtact ttcggcgttt gaagattgtt attttctttt ttctgtgccg
    14641 atatacgaca agtcggagat aaaaagaaat gtaaatccca agaaaatata ctgcgttgat
    14701 cattcaatga tgtcatcaat tgacccacag ataaatgaaa aaaaaggtca actgcttgaa
    14761 aatcttgttt tttcaacgat cagaagaagc tcaagcgcaa tttattatta taaaactgca
    14821 tccggaaagg aagttgattt ctgctggata gacagatccg gaaaaaagaa tcttgttcag
    14881 gtttcttttg acctaaacga tgaaaagaca agaaaaagag aagtttcagc actttttgag
    14941 gcaatgggtg agatcaatga aaaaagtgcg acacttgtaa caatgaatga atcaggtgtg
    15001 ttagaacttg aagacagaac aattcaaata gttccggcat gggaatatct gattaatttt
    15061 gaatatagtg aaaaaagtgt gtttcagata aacgatctga tgctttaaaa acagttgtga
    15121 tcactgattc gtcaaaaaat ccggtatgtt gaaatgtttt attccatcac ggctgaagtc
    15181 gaactgatcc attgagagga caaatttcgg atagttgtct ggaacttttt taagtacatc
    15241 aaattctctt ctgatggttt cttctgttgc aagaagataa gcaacttgaa tatatatggt
    15301 ttcattgttt tttctacata caaaatctat ttcttcgtta tgtactctgc cgatatttat
    15361 tttgtagccg cgtcttagca tttcaagaaa aacgatattt tcgagtacaa gattgatgtc
    15421 tctttcgttg tttccataaa ttgcttccct gatgccgtga tcagttatga aatatttttc
    15481 atttacctgc agaatctttt ttccgatgat gtcttctctt aaaactttat gaaaaagaaa
    15541 cgcctcctcg cagactttta tgtggttgag gatcgtctca ggagcaacgg tgcggttttc
    15601 actttttaaa aatctggaaa tgcttgttgc ggaaaatgtg tttccaacat ttgcaacaac
    15661 aaattgtata actcgttcaa gcagatctat gtctctgatc ttattccgct ttataacatc
    15721 tttgagtatg acggaatcaa aaacatcttt caaatatata aaagatgact g
//
LOCUS       DAOOYB010000043        15740 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_3673_length_15740_cov_9.709085, whole genome shotgun sequence.
ACCESSION   DAOOYB010000043 DAOOYB010000000
VERSION     DAOOYB010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 15740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15740
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3673_length_15740_cov_9.709085"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..937)
                     /gene="bioB"
                     /locus_tag="PL056_04095"
     CDS             complement(<1..937)
                     /gene="bioB"
                     /locus_tag="PL056_04095"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HPM46224.1"
                     /translation="MDFHKFIQNAKNGIKPTKDEAVEFLKLGAEYFNEIIYLGGTLRN
                     HFRGNRISTCTIINAKSGKCSENCRFCAQSAHHNSKIEIWDLLEKEEISEHAKVDAPF
                     GSYYGIVTSGRGLEAEDVEKVAETVEYFKKKEVPQKVCGSLGILSFEELVKLKKAGLQ
                     RFHHNLEVSRKFFPNLCTTHTWEERKETIENAKTAGLSLCVGGIFGVGESISDRVDLL
                     FEIAECDPDSVPLNFLVPIPGTPLENQPLFPLFEAVKIIVLARLIMPSKDIKIGGGRV
                     EIFKENHALPFLAGASSIITGQLLTVKGITPDDDIR"
     gene            complement(924..1979)
                     /gene="hydE"
                     /locus_tag="PL056_04100"
     CDS             complement(924..1979)
                     /gene="hydE"
                     /locus_tag="PL056_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013388214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HPM46225.1"
                     /translation="MILKDIIKLLSLKEKSDLEALYAAAYAEKIKHSGKKVFFRGLIE
                     FSNICAKDCFYCGIRKSNKNTGRYFLKMEEIIDVANFAYEQQYGSVVLQSGEREDDEF
                     VKFVEKVLREIKKIGNGALGITLSVGEQTEDVYKKWFDAGAHRYLLRVETSNRELYRK
                     LHPADHSFDRRVECLNILRKLGYQVGTGVMIGLPGQTIEDLANDILFFKETGVHMIGM
                     GPFIPHHQTPLADSIPEFDKVKDYQLELGLKMIAVTRLVLKNVNIASTTALQALSETG
                     RELGLKAGANIIMPNITETKYRKGYQIYDNKPSLNENAETTMKSLEMKINSIGEEIGY
                     NQWGDSPKFKEEKDGFS"
     gene            complement(1984..2910)
                     /gene="cysK"
                     /locus_tag="PL056_04105"
     CDS             complement(1984..2910)
                     /gene="cysK"
                     /locus_tag="PL056_04105"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005813707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HPM46226.1"
                     /translation="MVEKIFRSMLDLVGSTPLVKLNSISRETGAEVIGKLEYFNPMSS
                     VKDRIALAMIEDGEKRGVLNQNVTIVEPTSGNTGVGLAFVCAVKEYRLILTMPETMSI
                     ERRKLLKILGAELVLTPGEKGMKGAIEAAEKMAAENPGHIILQQFANPANPETHVRTT
                     AVEILNDCDGHVDIFVAGVGTGGTITGVGKVLKEKNPDVLIIAVEPDDSPVLSGGTPG
                     PHKLQGIGAGFVPAVLDKGVIDEVIRVTTDQAFSESRKIAREEGVFCGISSGAALSAA
                     RTVAARPENKGKRVVVIIPDTGERYLSTALFE"
     gene            complement(2922..3269)
                     /locus_tag="PL056_04110"
     CDS             complement(2922..3269)
                     /locus_tag="PL056_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46227.1"
                     /translation="MRYEVKKITKMIDEVLTFFIYNYNSCAKVDLMCQTDGYRIGFDF
                     PGLHISDEEIARIKKLLKAKRDPSLANYYWQLTGEVEDDCELPLVALMADKVDIKKDE
                     TGVKLIIYRKKLE"
     gene            complement(3259..3843)
                     /locus_tag="PL056_04115"
     CDS             complement(3259..3843)
                     /locus_tag="PL056_04115"
                     /inference="COORDINATES: protein motif:HMM:NF019244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HPM46228.1"
                     /translation="MLILTGFSISPVKEIPAGLMNIIYSPGILITDYLAVGGAGAAFV
                     NAGFCGLAASLMIVILKVNVNGPFIAAVFTVTGFAFFGKNIFNIWPVLIGVWLYAAYM
                     KTSFRNYLIVALFGTTLAPMVSYLAFGSGLSTSISVPVSMFFGILSGFVLPPLASHML
                     RFHDGYNIYNIGFTGGFSCSSYRCFEKKELKNAL"
     gene            complement(3899..5041)
                     /locus_tag="PL056_04120"
     CDS             complement(3899..5041)
                     /locus_tag="PL056_04120"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPM46229.1"
                     /translation="MTASSFKETGINTDLVKALAVQSITEPTEIQIQAIPELLKGIDV
                     IGCSETGTGKTLAYLLPLLMRIDQQKTSNQAIILAPTHELAIQIFREIEKLVTAGGLK
                     ITSAQCIGGANINRQIDKLKKKPHIIVGSTGRILELITAKKINPDIGTLVIDEADRML
                     DDNNFNLLLRLVRTLVKTARQTILFSASIGSETIKRAKAVTKDAVFIKTTQRIEINQN
                     LEHFYIECDKRDKILLVRKFYAAFADKKALVFVNNDVLVAELSEKLSFHNLKISAIHG
                     RFKKFERKTALENFRKGAARFLISSDISARGLDVKDIDFIINFDMPEESLDYLHRTGR
                     SARAGKVGSAVSFVTPPELKLLLKHSKHFKFELKKAQFHDGELHIK"
     gene            complement(5038..7923)
                     /locus_tag="PL056_04125"
     CDS             complement(5038..7923)
                     /locus_tag="PL056_04125"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HPM46230.1"
                     /translation="MRFSKRFKGFVFLFVAFASLSMFAETLVVMDIADKTGKIHPDVI
                     KGSKEYVIKLLSSVGKYTVIADAAVTDARKRSKEWTACSQMDCQIEIGKAFKADVIVV
                     PAVEYFAGIFTLTVNYIYVDGKRKVEAGATDFNGTAAGMKRALETVVSIIHGKKIEQQ
                     VFVEKNEALEKYKAENFKEAPKKEYVVPVEPMLIPIPPKILPGGVEFTSSHSGVRATV
                     DIGSQNSASCVVPCKVKDLNPGMHTVRFELKGYTTKDESFEIRQNETAKRSVSLTPVI
                     KSLEEINNELIQAAKEGKQKLVKELLEKGASVNYHDASGLSPLLIAALSGNRELAGYL
                     FGRGAKFSSVEAGTLMYVAVEKNDTWIASLVARNKSDLNITFDKGRTILWHAAQKEAW
                     DVFDQFVKSGVNLGIKDSTGSTIISWAMESGASNVIDRLNKLGVKTVPPDVDRMVKQA
                     VTNEDIDRLSSLVMMKPNLNAVYEDGLTLLWYASIKKRTDMVATLLKGGAKANVADKT
                     GRSLLMYCIETRRFEIAKLLKTYGAVLKNEDGVKLLQKGLVIGDSQLIQLLVQLNVNV
                     NIKFPDGLSALWIAAFTNNREIIEVLADAGANLNIKDKNGKTVLMWSVENDRKEVANI
                     LITRGANINLMDDTKKTALMSAVIADKPRMVHMLVKNGAKIDLKDAEGNSPMLVASAR
                     GNAGLVKLFLENGAFVNGVDGNGNTPLAISLHKNFDDITMLLLDKGADINKQNNLGES
                     PLMIVARKGQMRFVKIFVERGALIDSTDRDGNTALIHAKRENRREAVFYLLSKDAVIE
                     KRSEQDELMAFGTLNSYHDIVTNLISRNISVNRRFTDGLFPLYYAVRNGDSQMIDILI
                     AADADLEQRDKDGETVLLYASAKREEHVVVNLINKGANIAVTDKQKSTPLMIVAGRGF
                     AQAVKALIDKKADVNAKDDKGRTALVRAKFTGNYNIVDMLKRAGAYE"
     gene            complement(7997..8383)
                     /locus_tag="PL056_04130"
     CDS             complement(7997..8383)
                     /locus_tag="PL056_04130"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPM46231.1"
                     /translation="MTRKIKVLIVEDDRINQMVLSKMLELRGITTVCVSDGFEAVKYF
                     SEHDDLDIVLMDIQLPGMDGIETTAKMRKIEENKVKRTPVVALTAYFSTGNESEFLSQ
                     DFDDHAVKPVDYEFLIRLIKKHTIAS"
     gene            8511..11291
                     /locus_tag="PL056_04135"
     CDS             8511..11291
                     /locus_tag="PL056_04135"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HPM46232.1"
                     /translation="MKKVFLLIYIFLFSTISAEINGPFKNLIHRSWGSDEGLPQNSIY
                     TIFQDSKGFVWIGTAEGLVQFDGSSFKLYDKYASSAFKSNVILAIYESDDKCLYIGLR
                     NGGLVKKCGENFKQFSVDEGLTSNTVTSIVRSGDKLYLGTFGGKISVMQGDLIIPFPK
                     NSFIPDSFIHDMAVSSDGVLHVATDDGLYAINKDKVTRFGIDEGLPEQKIRNIFFDSG
                     KNLWVGTSDSGIAVFRNDGVTVYNKKNGLASDRVFAISEDSEGKIWVGTVGGGISVID
                     KGKISNFSKDDGLTSDIVRVLFKDRENNMWAGTFGGGLNLFRKGVFSSISSNNGLSDN
                     VIFALLESSNGDIYAGTYGKGLNIISPDGTVKIFNSSNGLSGDIPGAIHEDRSGRIWV
                     GSYGTGLDIIEKNGKISHFGHKDGLEMNTIVSIFEDSNGTVFLGGFDNSLTLFKNGAF
                     HSFSKEGLLKNRTVWAITQGIDGSILLGTDGAGIVKMKDNEFTVLDRESGLSDNKITA
                     IHKDSENIIWAGTYDNGINIITPEGKISHVKKEDGLFDDTIYSLVEDDEENLWMSSNK
                     GLFYVAKQDLLDLAEKKISSVRSKVFSWKDGMPSNECNGGFQKAGIKTRDGRLMFPTI
                     KGVAVMDPSIKKEMPFVPHPVITSVTVDGKRVENVSRIVLEPGVSKIRMEYTAPFFST
                     PQKIEFRYKLSGFDRNWIYAGKSRFAEYMNLNPGEYVFEVSVSDMDGNWNEGTEKISI
                     IQEPAFHQHMLFRLLMLLIAGVLIYIPVNRKIRRMKIKNEELSDRITETEEEIKQISE
                     ELESKYASSSLSEEDMDFYKETLEKYMKSEKPYLDNELSIRKLAQQLEMQPHHLSQVI
                     NSGFDNNFYTYINNYRINEVIDLMKDPKRKHHTILAIAYDSGFKSKSSFNTIFKKMTG
                     KTPSEFRDELES"
     gene            complement(11311..14229)
                     /locus_tag="PL056_04140"
     CDS             complement(11311..14229)
                     /locus_tag="PL056_04140"
                     /inference="COORDINATES: protein motif:HMM:NF013507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46233.1"
                     /translation="MKYLFFLIFILFAGCGSNSAANDGDINDEEKDSDTILDSDSYFD
                     SDIENADERDDDDLVDNNTLVNLSDWERVSDGFEFNYYSNRSITFLNGQYYLVSGDEV
                     YSSVDGINWLTVCNNAPFKSRKGHSVVKFKENMLVVGGGVYTSLGDIWYSKDGVVWDI
                     LTRFAPFGARTNLSAIEFKDSLWILGGSFVHNTCEVCSGSEIWSTNDLENWEIKVSEA
                     PFGNLYSQELIEHKNELWLFATHDENTKNSVWRSTDGISWESIADVLPFDARSSVSFY
                     SWKGKLWLAGGYGSDYNYFSDVWSSEDGVEWVKTGNFEEFGGRYKSTVIANEKEVLLI
                     SGYQQKSANLKQILSTEDGVNWKLKWGSEYLPDRFAHTVDRFKDKFWVIGGRIRYSED
                     GVELRDVYSSEDGRKWDRVVSNAPFGSRIGHASVVYKDRIWIFGGYFSSGPYNGGDVW
                     SSKDGVDWNVDSVKAPFHLRSIDDAVVYEDEMVLVGGVSDSAEKVLWYSKDGVNWRKG
                     ENIDFAECDNSKMVVHEGSLFLIGGCTEDRYGNEVYRKNDEGDWIKLETENVFSPRRA
                     HRVASMGGFIWVIAGKDGDDLNDVWYSKDGKKWYQEKNAAFSPRAEFDILSYDSKIWV
                     FGGGGSAENSHDYGDVWVVEIESVKQREETPENDSENPDFNDIKWKSAGDDIPSERFD
                     HSFLKTDDGFYIIGGRLWGDDLYEIENSAIFSSDGASWEILITNEVLLDKIGVAYVYF
                     KDKIWGIGGNSDSIDFYGQAVTNQIIFSENGKDWQIAVESPEITPRYNHKAVVFKDRI
                     WVVGGFDADENGLDDVWSSEDGINWKREVSVSDFRGRGVHGLHVLNDRIYMIGGMRNS
                     WDNGSSEVWSSEDGISWERDSYLGFSQRRMFGSTVASGVLWVFGGAGREGELNDLYYS
                     SDGIKWNKAECNYVVSKRRGSVILGDDNRLWLSGGVFTDSSYVAHTYKDVWFADISIK
                     "
     gene            complement(14300..14947)
                     /locus_tag="PL056_04145"
     CDS             complement(14300..14947)
                     /locus_tag="PL056_04145"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPM46234.1"
                     /translation="MKVRSVIFDMDGTLLNTMEMIVKCNNSVLKSEGYPERKFDEFFN
                     FVGDGMKQCVTRALPAGTDEVVITDVLSKVLEKYKSEDVSTIKTYEGVDELLKVLVSK
                     GIKISILTNKEHDYAVVNAQIALVDHRFEAVIGDRPHTPLKPDPAGVFEIAEITGIPL
                     TETIFVGDMTADILTGKNAGVFAVGCLWGFGNKKDLENAGADLLIEHPLELLKII"
     gene            complement(14976..>15740)
                     /locus_tag="PL056_04150"
     CDS             complement(14976..>15740)
                     /locus_tag="PL056_04150"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPM46235.1"
                     /translation="GCPFHCVYCSYPVIEGKKVRALEPHNVVETIKRSVEKGLDYFFF
                     TDSVFNMNPEYNRELAELIIKNDIKVKWGAYFRPAGTSFEDLELYKKSGLTHIEFGTE
                     SFSDPVLKSYGKEFSFEDIKNYTQMCRELKIYNAHFLILGGYGETEETLEQTFERSKL
                     LPWTVFFPFIGMRIYPHTPLYKIALNEGVITADDDLMESKYYLADGVDLSMKKLKDQA
                     VATGRKWVFPDEDHSKILKKMKSRGKRGPLWEFLVN"
BASE COUNT         4477 a         3654 c         2809 g         4800 t
ORIGIN      
        1 gccggatatc atcgtccgga gttattcctt taacggtaag aagctgtcct gtgattatgg
       61 aactagcacc tgcaaggaat ggaagtgcat gattttcttt aaatatctca actctgccgc
      121 ctcctatctt aatatccttt gaaggcatga tgagccgcgc cagcactatt attttcactg
      181 cttcaaaaag cgggaaaagc ggctggttct caagcggagt tccgggaata ggaacaagaa
      241 aattaagcgg caccgaatcg ggatcgcatt cagctatctc aaaaaggaga tctactctat
      301 ctgaaattga ttcccctact ccgaaaatgc caccgacaca aagcgacaat cccgcagttt
      361 tagcgttttc tatggtctct tttcgctctt cccatgtgtg agttgtgcaa agattcggaa
      421 aaaatttccg tgaaacttca aggttgtgat gaaacctctg aagtcccgct ttttttaatt
      481 ttacaagctc ctcaaaagac aagatcccga gtgacccgca aactttctga ggaacttctt
      541 ttttcttaaa atattcaaca gtttcagcaa ctttctcaac atcttctgct tcaagacctc
      601 tgccagaagt gacaatcccg tagtaacttc caaatggagc gtctaccttc gcatgctcag
      661 agatctcttc cttttcaagc agatcccata tttctatttt tgaattatga tgggcactct
      721 gggcacagaa ccggcagttt tcagaacatt tgccgctttt tgcattaatt attgtgcatg
      781 tagagattct gtttcccctg aaatgattcc ggagcgttcc acctaaatag ataatttcat
      841 taaagtattc agcaccaagc tttagaaact caactgcttc gtcttttgtc ggttttattc
      901 cgttttttgc gttttggata aatttatgaa aatccatctt tctcctcttt gaatttcggt
      961 gaatcgcccc actggttgta accgatctct tcaccgatag agtttatttt catttcgaga
     1021 cttttcatgg tcgtttccgc attctcattt agactcggct tgttatcata aatctgataa
     1081 cctttcctgt attttgtttc ggtaatattc ggcataatta tatttgctcc ggcttttaaa
     1141 ccgagttctc ttcctgtttc actcagggcc tgcaaagctg tggttgaagc aatgttgaca
     1201 ttcttaagca cgagtcgggt cactgctatc attttgagtc caagttcaag ctgataatct
     1261 ttaactttat caaattcagg gatcgaatcg gcaagcggag tctgatgatg aggaatgaac
     1321 ggacccattc ctatcatatg aacaccggtc tccttgaaaa agagaatatc gtttgcaaga
     1381 tcttcaatgg tttgaccggg aagaccgatc attacccctg tccctacctg ataacccagt
     1441 tttctcaaga tgttaagaca ttcgaccctt ctatcaaaac tgtggtccgc agggtgaagt
     1501 tttctgtaaa gttctctgtt tgaggtttca actctcaaaa gatatctgtg tgctcccgca
     1561 tcaaaccatt ttttatagac atcttctgtc tgctcaccca ccgaaagagt tatccccaga
     1621 gcaccattcc cgatcttctt tatttcgcga agaacctttt ctacaaattt tacaaactca
     1681 tcatcctccc tttctcccga ttgaagaact acagaaccgt attgctgctc ataggcaaaa
     1741 ttagccacat caatgatctc ttccattttg agaaaatatc ttccggtgtt tttatttgac
     1801 tttctgattc cgcagtaaaa acagtccttt gcacatatat tgctgaactc aataagacct
     1861 ctgaaaaaca cttttttccc actgtgttta attttttcag cataagccgc cgcataaaga
     1921 gcttccagat cggatttttc tttcagcgac aaaagtttta ttatatcttt taaaatcata
     1981 ttcttactca aaaagtgcag ttgacaaata cctttcgcct gtatcaggaa tgatcacgac
     2041 aacccttttc cccttgtttt caggtcttgc ggcaactgtt cttgccgcag aaagtgccgc
     2101 tcctgaagaa attccgcaga aaacaccctc ctctcttgca atttttctgc tttcagaaaa
     2161 tgcctgatct gttgtaactc tgatcacttc atcaatgaca cctttatcaa gaacggcagg
     2221 gacaaaacct gctccgatcc cctgaagttt gtgaggtccg ggggttccgc cgcttaagac
     2281 tggcgaatca tctggttcaa ctgcgattat taacacatca ggatttttct ctttcagcac
     2341 ttttccgaca cctgttattg ttccaccggt acccacacca gcaacaaaaa tatcaacatg
     2401 cccgtcacag tcgttcaata tttcaactgc cgtcgtcctg acatgtgttt cgggatttgc
     2461 agggtttgca aactgctgaa gaattatgtg ccccggattt tcagcagcca ttttttccgc
     2521 cgcctcaatt gcccccttca tccctttttc acccggagtc agaacaagtt cagcccctaa
     2581 aattttaaga agttttcttc tttcaatgct catcgtttca ggcatcgtta gaataagtct
     2641 atactctttt acagcacata cgaaagcgag acccacaccg gtattgccgc ttgtcggctc
     2701 aactatcgtt acattttgat tgagcacacc tcttttttca ccatcttcga tcattgcaag
     2761 tgcaattcta tccttgacac ttgacatagg attgaaatat tcaagtttac cgataacttc
     2821 cgccccggtt tccctgctga tcgagttgag ctttacaagg ggcgtacttc cgacaagatc
     2881 aagcattgat ctgaaaatct tttcaaccat gatcaacctc cttattcgag tttttttctg
     2941 taaataataa gtttcacacc ggtttcatct tttttgatat caactttatc ggccatcaga
     3001 gcgacaagag gaagttcaca gtcatcttcc acttcgcctg taagctgcca gtaatagttt
     3061 gcaagagagg gatctctttt cgctttaaga agttttttta tcctggcgat ctcttcgtct
     3121 gagatatgca atcccggaaa atcaaatcct atcctgtatc catctgtctg acacatgagg
     3181 tcgacttttg cacaggagtt gtaattgtaa atgaaaaatg taagcacttc gtcgatcatt
     3241 tttgtgattt ttttcacttc ataacgcatt tttcaactcc tttttctcaa agcatcgata
     3301 agaggaacaa gaaaacccgc cggtaaaacc gatattgtat atgttgtatc cgtcgtggaa
     3361 acggagcatg tgagaagcca gaggcggaag aacaaatccg gaaagaattc cgaaaaacat
     3421 tgatactgga actgatatcg aagttgaaag ccctgatccg aacgcaagat aacttaccat
     3481 aggagcaagt gttgttccga aaagtgcaac aatgagataa tttctgaaac ttgtcttcat
     3541 atatgctgca tataaccaga cccctataag cacaggccag atgttaaaaa tgttcttccc
     3601 gaaaaaagca aatcccgtaa cagtgaaaac cgcggcaatg aaaggtccat tgacattcac
     3661 cttcaaaatt acaatcatca gggaagcagc cagtccgcaa aacccagcat ttacaaaagc
     3721 cgcacccgcc ccgccgacag ccagatagtc ggttatcaga ataccgggag aataaattat
     3781 gttcattaga cctgcaggta tttccttaac cggagaaatc gaaaaacctg tcaggatcaa
     3841 cattagcgga agagcaacta ataaagggta tttaaagtgt tctttcatgt caccggtttc
     3901 attttatgtg aagttcacca tcatggaatt gcgctttttt caactcgaat ttaaaatgtt
     3961 ttgaatgttt taaaagcagt ttaagttcag gcggtgtgac aaaagaaact gcagatccga
     4021 ccttgcctgc ccttgcgctt cttcctgttc tatggagata atcgagagat tcttcaggca
     4081 tatcaaaatt tataataaaa tcaatgtctt taacatcaag tcctcttgct gaaatatcag
     4141 aactgataag aaatctggca gctcctttcc tgaaattttc aagtgccgtc ttcctttcaa
     4201 attttttgaa tctaccgtga attgccgaaa tcttgaggtt gtgaaacgaa agcttttcag
     4261 aaagctctgc aacaagaaca tcattattta caaaaacaag agctttctta tctgcaaaag
     4321 cggcatagaa ttttctgaca agaagtatct tatccctttt atcgcactca atgtaaaaat
     4381 gttcaagatt ctgattaatt tcaattctct gagtggtttt tataaacact gcgtcttttg
     4441 tgactgcttt tgccctttta atagtttcac tcccgatcga tgcagaaaaa aggattgtct
     4501 gtctcgctgt tttcacaagg gttctgacaa gccgcagaag gagattgaaa ttattgtcgt
     4561 caagcattct gtcagcttca tcaattacaa gcgtgccgat gtcagggttt atcttttttg
     4621 cggttatcaa ttcaagaatt cttccggtgg atccgacaat gatgtgaggt ttctttttca
     4681 acttgtcaat ctgtctgttt atattagcac caccgatgca ctgagctgaa gtgatcttca
     4741 accctcctgc agttacaagc ttttctatct ctctgaatat ctgtatcgcg agttcatgcg
     4801 tgggggccag tattattgcc tgatttgatg tcttctgctg atctattctc atcaaaagag
     4861 gcaggagata ggcgagcgtc ttgcctgttc ccgtttctga acaaccgata acatctatcc
     4921 ctttgaggag ctccgggatc gcctgaatct gtatctcagt tggttctgta attgactgga
     4981 ccgcaagggc ttttacgaga tctgtgttta tcccggtctc tttgaatgat gatgccgtca
     5041 ttcgtatgca ccggctcttt tcagcatatc tacaatgtta taattacctg tgaacttagc
     5101 cctgacaagt gcggtacgcc ctttatcgtc ctttgcgtta acatccgcct ttttgtcaat
     5161 aagagctttt accgcctgtg caaaacctct gccggccact atcatcagcg gagtgctttt
     5221 ctgtttgtct gttactgcaa tgttcgcacc tttattaata agattgacaa caacatgttc
     5281 ttctcttttt gccgaagcat agagcagtac cgtttcgccg tccttgtctc tctgctcaag
     5341 gtcggcatca gccgcaataa ggatatctat catctgactg tcaccgttcc tgactgcgta
     5401 ataaaggggg aaaagaccat ctgtaaaacg gcgattgacc gaaatgttcc ttgatatgag
     5461 atttgtaact atgtcatgat agctgttaag tgtcccgaaa gccataagct catcctgctc
     5521 gctccttttt tcaataacgg catccttgct cagaagataa aatacggctt ctctcctgtt
     5581 ctccctcttt gcatgtatga gcgctgtatt tccgtctctg tccgttgagt ctatgagcgc
     5641 ccccctttca acgaatatct ttacaaaacg catctgccct tttcttgcta cgatcatcag
     5701 gggcgattca cctaaattgt tctgtttgtt gatatcagca cctttatcaa gaagcagcat
     5761 ggttatgtcg tcgaaattct tatgaagcga tattgcaagc ggagtgttgc cgttgccgtc
     5821 gactccgtta acaaatgctc cgttttcaag aaaaagcttt acaagtccgg cattacccct
     5881 tgctgatgcg acaagcatcg gagagttgcc ctcagcatct ttgaggtcta tctttgctcc
     5941 gttcttaaca agcatgtgca ccattctcgg tttatcggca attactgcag acatgagtgc
     6001 cgtttttttg gtatcgtcca tgaggttgat gtttgcacct cttgttatga ggatgttggc
     6061 aacctctttt ctgtcatttt caacagacca cataagcaca gtctttccat tcttgtcttt
     6121 tatgttcagg ttcgcgcctg catcagcaag aacttctatt atctcacggt tgttcgtgaa
     6181 agctgcgatc cacaatgcac tcagaccgtc cgggaattta atgttcacgt tgacattaag
     6241 ttggacaaga agctgaatga gctgactgtc acctattaca agacctttct gcaaaagctt
     6301 cacaccgtct tcgttcttta aaacggctcc gtatgtcttt aaaagtttcg ctatttcaaa
     6361 acggcgtgtc tcgatgcagt acataagcag tgacctgccc gttttgtctg caacatttgc
     6421 ttttgctcct cccttaagaa gagtcgcgac catatcagtt cttttcttta ttgatgcgta
     6481 ccacagaaga gtaagaccgt cttcatacac agcattcaga ttcggcttca tcatgacaag
     6541 agagctgagt ctgtcaatat cctcgtttgt gaccgcctgt ttcaccatcc ggtcaacatc
     6601 aggcggaaca gttttaacgc caagcttgtt gagccggtca attacatttg aagctccgct
     6661 ttccatagcc cacgatatta ttgtgcttcc cgttgaatct tttattccga ggttgacccc
     6721 gctttttaca aactgatcaa agacatccca cgcctctttc tgtgcagcat gccacaaaat
     6781 cgttctcccc ttatcaaatg tgatattaag gtcggattta ttccttgcga caagagacgc
     6841 tatccaagta tcgttctttt caacagcgac atacatcagg gttccggcct caacagaaga
     6901 aaatttcgcg cctcttccga aaagataccc tgcaagctcc ctgttaccgc tcaatgcagc
     6961 aataagaaga gggcttaacc ctgaagcatc atggtaattt acacttgcac ccttttccag
     7021 aagctccttg acaagcttct gctttccttc ttttgccgcc tggataagtt cgttattaat
     7081 ctcttcaaga ctttttatta ccggtgtaag cgaaacactc ctctttgcag tttcgttctg
     7141 tctgatctca aacgattcat cttttgtcgt gtaacctttc agttcaaaac ggacagtatg
     7201 cataccggga ttgaggtctt ttactttaca gggaacaaca catgatgcag aattctgtga
     7261 accgatgtca actgtcgccc tgactccgct gtggcttgat gtgaattcaa ctccgccggg
     7321 aagtattttt ggcggtatcg gaataagcat cggctcaaca ggaacaacat attccttttt
     7381 tggcgcctct ttgaaatttt cagctttata tttttcaagt gcttcatttt tctcaacaaa
     7441 aacctgctgt tctatcttct tgccgtgaat tattgaaaca actgtctcca gcgctctttt
     7501 cattcccgca gcagttccgt tgaaatctgt ggcccctgct tcaacctttc ttttcccgtc
     7561 aacataaatg tagttaactg tcagggtgaa tataccggcg aaatattcaa cagcagggac
     7621 aactataaca tccgccttga atgctttacc tatttctatc tggcaatcca tctgagaaca
     7681 tgctgtccac tcctttgacc gttttctggc atcagtcacg gcagcgtctg caataaccgt
     7741 atattttccc acactgctta aaagcttgat gacatattcc ttagaccctt ttatgacatc
     7801 agggtgtatc ttccccgtct tgtcggctat atccatcacc acaagagttt cagcgaacat
     7861 gctcagagaa gcaaaagcga caaacagaaa aacaaaacct ttgaaccgtt tagagaacct
     7921 catatttttc ctccgatcac gacacattat cggattaaat atactataaa gtcatatcta
     7981 ttcaataaaa ttcttatcag gacgcaattg tatgtttttt tataagccgg ataagaaatt
     8041 catagtcaac cggcttgaca gcatgatcat caaaatcctg tgaaagaaat tcactttcat
     8101 ttcctgttga aaaataggcg gtcaatgcca caacaggcgt tcttttcacc ttgttctcct
     8161 ctatttttct catcttggca gttgtttcga taccatccat tccgggaagc tgtatatcca
     8221 taagaacgat atcaagatcg tcatgctcac tgaaatattt gacggcttca aacccatccg
     8281 aaacacagac tgttgttatt cccctgagtt caagcatttt tgacagtacc atctgattta
     8341 tcctgtcatc ttcgacaata aggaccttta tcttcctggt cataaaaata atctccgaaa
     8401 atacaaacga agtatagcat ttatcaaaaa tacaggcaag ataattactt gcttgatttt
     8461 aaaaaaatat taagttaaca tcagttgtat taattgtgtg tcttacagta atgaaaaagg
     8521 tttttctgct tatatacatt tttctgtttt cgacaataag tgcggaaatc aatggtccgt
     8581 tcaaaaatct catacataga agctggggat cagatgaagg actgccgcaa aactcaattt
     8641 acactatctt tcaggattcg aaaggatttg tctggatagg aaccgctgaa ggtcttgtac
     8701 aatttgacgg atcgtcattc aagctttatg acaaatatgc ctcttcggca tttaaaagca
     8761 atgtcatact tgcaatatac gaatctgatg acaagtgtct ttacatcgga cttcgcaacg
     8821 gcggtcttgt aaaaaaatgc ggcgaaaatt tcaaacagtt ttctgtagat gaagggctta
     8881 catccaatac ggtaacttcc attgtacggt ccggcgacaa gctttatctt ggaacattcg
     8941 gtggaaaaat atcggtaatg cagggcgatt tgataatacc ttttccaaaa aacagcttca
     9001 ttccggattc gttcatacac gacatggccg tatcttccga cggagtgctt catgttgcta
     9061 cagatgacgg actgtacgct ataaataaag ataaggtgac ccgtttcgga attgatgaag
     9121 gacttcctga acagaagatc cgcaacatat tttttgattc cggaaaaaat ctctgggtcg
     9181 gcacaagtga ctcaggtata gccgttttcc gtaacgatgg agttaccgtc tacaataaaa
     9241 agaacggtct tgcttcagac agagtttttg caatttctga agatagcgaa ggaaaaatat
     9301 gggtaggaac ggtcggagga ggcatctccg ttattgacaa aggcaagatc tccaacttca
     9361 gcaaagatga cggtctcact tcagatattg tcagagtcct tttcaaagac agagagaaca
     9421 acatgtgggc cggaacattc ggcggcggtt tgaatctttt cagaaaaggt gttttcagtt
     9481 caatatcatc aaataacggt ctgagcgaca atgtaatatt cgcccttctt gaaagttcaa
     9541 acggtgacat ttatgcagga acatacggaa aaggtctcaa catcatcagt cctgacggaa
     9601 ctgtaaaaat atttaactcc tcaaacggtc tttcgggcga cattcctgga gccattcacg
     9661 aagaccgcag cggcaggatc tgggtgggtt catacggaac aggtcttgac atcattgaaa
     9721 agaacggaaa aatatcgcat ttcggtcata aggacgggct tgaaatgaac actattgtct
     9781 caatatttga agacagtaac ggaacagtgt ttctgggcgg atttgacaac tctttgactt
     9841 tattcaagaa cggggctttt cattcttttt caaaagaggg gctgcttaag aacagaacgg
     9901 tatgggcaat aacacagggg atcgacggat caatacttct cggaactgac ggagcaggta
     9961 tagttaaaat gaaagataac gagttcactg ttctggacag ggaaagcgga ctcagcgaca
    10021 acaagatcac tgcaatacac aaagatagtg aaaacattat ctgggctggt acttacgaca
    10081 acggcataaa cataatcact cctgagggaa agatatctca tgtaaaaaaa gaggacgggt
    10141 tatttgatga tacaatatac tcccttgtcg aagatgacga agaaaatctc tggatgagtt
    10201 ccaataaagg ccttttctat gttgcaaaac aggatttgct tgatcttgcc gaaaagaaaa
    10261 tttcctctgt aagatcaaaa gttttttcat ggaaggacgg tatgccttca aatgaatgca
    10321 acggcggatt ccagaaagcc ggaataaaaa cccgcgacgg acggctgatg ttccctacga
    10381 tcaagggagt cgccgtcatg gacccgtcaa ttaaaaaaga gatgccgttt gttccgcatc
    10441 ctgtaataac ttctgtaacg gttgacggaa aaagagtgga aaatgtttca cgcatagtcc
    10501 ttgagccggg agtttccaaa ataaggatgg aatatacagc cccttttttt tcaactcccc
    10561 aaaaaataga gttcagatac aaactttccg ggtttgacag gaattggata tatgcaggaa
    10621 agtcgaggtt tgcggaatat atgaacctta accccggaga gtatgttttt gaagtgagtg
    10681 tgtctgatat ggacggcaac tggaatgaag gaacggaaaa gatctcaata attcaggaac
    10741 ctgcattcca ccagcacatg ctgttcagat tactgatgct gctcattgca ggtgttctta
    10801 tctacatccc ggtaaacaga aagatcagga gaatgaaaat aaagaatgag gagcttagtg
    10861 acagaataac cgagacagaa gaggagatca agcagataag cgaggagctc gaatccaaat
    10921 atgcttcaag cagtctaagc gaagaggaca tggattttta taaagagact cttgaaaaat
    10981 atatgaaaag tgaaaaaccc tatcttgaca acgaactcag catcagaaaa ctcgctcagc
    11041 agcttgaaat gcagccgcat cacctttcac aggtcatcaa cagcggattt gacaacaatt
    11101 tctatacata tatcaacaac taccggataa atgaagttat agacctgatg aaggatccta
    11161 aaagaaagca ccacacgatc cttgccatcg cctatgattc cggattcaaa tcaaaatcat
    11221 cgttcaacac aatattcaaa aaaatgacag gaaaaacccc gagcgaattc agggatgaac
    11281 tggaatcata aagccgattc ttacaatttt ttattttatg ctgatatctg caaaccagac
    11341 atctttatat gtgtgtgcaa catatgaaga atccgtgaaa accccgccac tcaaccaaag
    11401 ccggttgtca tctcccagaa ttacagatcc tcttctcttg ctcaccacat aattacattc
    11461 tgctttgttc cactttattc cgtcagatga gtaataaaga tcattaagct ctccttcacg
    11521 cccagctcct ccaaaaaccc agagcacacc ggacgcaaca gtcgaaccaa acattctcct
    11581 ttgtgaaaaa ccaagatacg aatctctttc ccagctaatg ccatcttctg aagaccatac
    11641 ttctgaagaa ccgttatccc aactgtttct cattccgcct atcatataaa ttctatcgtt
    11701 taaaacatga agtccgtgaa cacctctacc cctgaaatcc gaaactgaaa cttctctttt
    11761 ccagtttatt ccatcttcag aactccacac atcatccaag ccattttcat cagcgtcaaa
    11821 accaccaaca acccagatcc tatctttgaa aactactgct ttatgattat agcgtggagt
    11881 aatctccgga ctttccacag caatctgcca gtcttttcca ttttcggaaa atattatttg
    11941 atttgtcacg gcttgaccat aaaaatcaat gctgtccgaa tttcctccta ttccccaaat
    12001 cttatctttg aaataaacat aggccacgcc tattttgtca aggagcactt catttgttat
    12061 caatatttcc caggaagcac cgtcactgga aaaaatcgca gaattttcaa tttcataaag
    12121 atcatctccc caaagtcttc cgccgataat ataaaaaccg tcatctgttt tcagaaagct
    12181 gtgatcaaat ctttctgacg gtatatcatc tcctgctgat ttccatttta tatcattgaa
    12241 atccggattt tcgctgtcat tttcaggagt ttcttctcgt tgctttaccg actcaatttc
    12301 aacaacccat acatcaccgt aatcatggct gttttcagca gatccgcctc caccgaacac
    12361 ccaaatcttt gaatcatagg acaaaatatc aaattccgct cttggactaa aagcagcgtt
    12421 tttttcctga taccattttt tcccgtcttt tgagtaccac acatcattaa gatcatctcc
    12481 gtctttaccg gctataaccc agatgaatcc gcccattgaa gcaactctgt gtgctctcct
    12541 tggagaaaaa acattttctg tttcaagctt aatccaatca ccttcgtcgt ttttccggta
    12601 aacctcgttt ccatatctat cttcagtaca gccgccaatt aaaaaaagag acccttcgtg
    12661 tactaccatt ttgctgttat cacattcagc aaaatcaata ttttctcctt ttctccagtt
    12721 aactccgtct ttagaatacc acaatacttt ctcagcacta tccgaaactc cacctacaag
    12781 caccatttcg tcttcataaa caacggcatc atctattgat cttaaatgga aaggggcttt
    12841 aactgaatct acattccaat caacgccatc tttactgctc caaacatctc cgccattgta
    12901 aggaccactt gaaaaatatc ctccgaatat ccaaatcctg tctttatata ctactgaagc
    12961 atgtccgatc cggcttccga atggagcatt agaaaccact ctatcccatt ttctgccatc
    13021 ctcagaagaa taaacatctc tcagttcaac accgtcttct gagtatctta ttcttccacc
    13081 gatcacccag aacttatctt taaatcggtc aacagtatgt gcaaatctat caggaagata
    13141 ttcagagccc cactttaatt tccagttaac tccatcttct gttgagagaa tttgttttaa
    13201 atttgctgat ttttgctgat atccgcttat taaaaggact tctttctcat ttgctattac
    13261 ggtgctcttg tatcgaccgc caaactcttc aaagtttccg gtttttaccc attccacacc
    13321 atcttcagat gaccacacat cgctaaaata attataatca ctgccatatc caccagctag
    13381 ccaaagcttt cctttccacg aataaaaact gacactgctt ctagcatcaa aaggcaaaac
    13441 atctgctata ctttcccagc ttattccatc agttgaccgc catacactgt tttttgtgtt
    13501 ttcatcatgt gttgcaaata gccatagttc atttttatgt tctataagct cctgtgaata
    13561 caaatttcca aatggagctt ctgacacttt tatttcccag ttttccagat cgtttgttga
    13621 ccatatttca gaaccgctac acacctcaca ggtattatgt acgaaacttc ctcctaaaat
    13681 ccaaagacta tccttaaatt caattgcact taaattcgtt cttgctccaa aaggagcaaa
    13741 tctggtcaaa atatcccaga caactccatc ctttgaatac cagatatccc caagagatgt
    13801 gtatacgcca cctccgacaa caagcatgtt ttctttaaat tttaccacag aatgcccttt
    13861 tctcgattta aaaggggcat tattacaaac tgtcaaccaa tttatgccgt caacggatga
    13921 atacacttca tcacctgaaa ccagataata ttgtccattt agaaatgtaa ttgatctgtt
    13981 gctataataa ttaaactcaa aaccgtctga aactctctcc cagtcagaaa gatttacaag
    14041 agtattgtta tccaccaagt catcgtcatc tctttcatcc gcattctcaa tatcactatc
    14101 aaaatacgaa tcagaatcca gaatagtatc agaatctttt tcttcatcat taatgtcacc
    14161 gtcattcgct gcagaattag aaccgcatcc tgcgaataaa ataaaaatca gaaaaaacaa
    14221 atacttcata taattcccca taaaggttga cattatctca gatattctat ttaaaaatgt
    14281 ttaaaaatgt caaatattat cagataattt tcagcagttc cagcggatgc tctatcaaca
    14341 gatcggctcc cgcattttca aggtcttttt tattgccgaa tccccagaga catccgactg
    14401 caaaaacccc tgcattcttg ccggttaata tatcagctgt catgtcgccg acaaaaattg
    14461 tttctgtgag aggaatccct gtaatttcag ctatttcaaa aacaccggca gggtctggct
    14521 tcaacggagt atgcggtctg tcacctatca ccgcttcaaa cctatggtcc acaagagcta
    14581 tttgagcgtt aacaactgca taatcatgct ccttgttggt aagtatggat atctttatcc
    14641 cttttgaaac aagcactttg agcagttcgt caacaccttc ataggttttt atcgttgaga
    14701 catcctcgct tttatatttt tcaagcactt ttgaaagaac atctgttatt acaacttcat
    14761 cggttcccgc aggaagtgcc ctcgttacgc actgcttcat tccgtcaccg acaaaattaa
    14821 aaaattcgtc gaattttctt tcaggatatc cttcgctttt aagaacggag ttgttacact
    14881 tgactatcat ttccattgtg ttgagcaaag ttccgtccat atcaaatatc accgatctta
    14941 ctttcattat tagttctcca ttttacggat gtttttcagt ttacgagaaa ttcccagagc
    15001 ggtccccgtt tgccgcggct tttcattttt ttaagtatct ttgaatggtc ctcatcggga
    15061 aaaacccatt ttctacctgt tgcgaccgcc tgatctttaa gttttttcat tgaaagatca
    15121 acaccgtctg caagataata ttttgattcc ataagatcat catcagctgt tattacccct
    15181 tcattaagag caattttata aagcggtgta tgaggataaa ttctcatccc gataaacgga
    15241 aaaaagactg tccacggaag aagttttgac ctttcaaaag tttgctcaag agtctcttcg
    15301 gtttctccat atccgcccag aataaggaaa tgtgcattgt aaattttaag ttcacgacac
    15361 atctgagtgt agttttttat atcttcaaaa gagaactcct tgccgtaact tttaagcacc
    15421 gggtcgctga acgattccgt tccgaattca atgtgggtaa gtccgctttt cttataaagt
    15481 tcaaggtctt caaaacttgt tcccgcaggc ctgaaatatg caccccattt gaccttaata
    15541 tcattcttta ttatcaattc agcaagttcc ctgttatatt ccgggttcat gttaaagacc
    15601 gaatcggtga aaaagaaata atcaagccct ttttcaacag atctttttat cgtttcaaca
    15661 acattatgcg gctcaagggc tcgcactttt ttcccctcga taacgggata cgagcagtaa
    15721 acacagtgga aaggacagcc
//
LOCUS       DAOOYB010000044        15487 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_3782_length_15487_cov_9.535835, whole genome shotgun sequence.
ACCESSION   DAOOYB010000044 DAOOYB010000000
VERSION     DAOOYB010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 15487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15487
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3782_length_15487_cov_9.535835"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            80..2113
                     /locus_tag="PL056_04155"
     CDS             80..2113
                     /locus_tag="PL056_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46236.1"
                     /translation="MKKFSVFLIVIAVVIFTSCDKNKGDDFTYGDYPDSVNKNDEDDY
                     NYVKKDADTVKKDDDSYTSTDNDTVSDPDDDTFILTDEDNFTLPDDDNFTLPDEDGSV
                     LADEDNFSFPDEDNYILPDEDNSTLPDEDSTVTPDNAVADIDTYVEPECKNGIVEDGE
                     ICEPTIPKQCHTLNPEIYQSGWATCEDDCSGWIMTSCVEYPCSEKPDVPDSLFTDSNC
                     DGIDGNIIESVFVDPVFGSNMNDGTMSLPVATITKGIEIAQNTGKKHVLVSGASYSEN
                     VVLKTGISVHGGYSGNPNWTRSTMFETIIQTGVNGILAEWVGDFTLSFLTIRSANAVS
                     AGQSSFGMVLKNCENITLSNVKVIAGNGAPGTDGTDGVKGGDGTQGTQGNQGCESSGG
                     FFCDSPCSAPEGGAGGSSPCGANGGKGGTPGKGSSNGYPGENGVGSTDNGGDGDIAGS
                     WDGMCLYYEGLAVHGGDGELGSFGVNGTGGGTLGTVSESGYVPADGGNGTDGEHGQGG
                     GGGGGGCGGTDACNSYGSSGGGGGGGGCGGTAGTKGTGGGGSFALWLYKCTDIVFETV
                     RLESKNGGAGGKGGNGMSGGFGALGGAGGTYGGDGDQDDGGCGGWGGAGGSGGTGGSG
                     GGGGGGPSIPMVKIQSSVEGMYFATYVFGSGGAGGPSNGNVGLNGRAGQYYED"
     gene            2136..>3026
                     /locus_tag="PL056_04160"
     CDS             2136..>3026
                     /locus_tag="PL056_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HPM46237.1"
                     /translation="MKNTSILFLVVFFVFACTPDKDDSIYDYPDNTVVKNDEDSSVKN
                     DNTVINDSDSQLPDTHVLPDGEQPDTLTPDNESTDDPFIATCGNGIVDFGEVCERDTE
                     KECSAIDPGSYVDGVAKCNDSCSGWIVSDCVSVGCETAVDNPDDAFADTNCDGIDGDA
                     SASVFVDKLNGLNTNPGTKDLPVATIMKALEIAQEQDKKHILVGTGAYSEQVELKEGI
                     SIHGGYSGHPNWARGVEYEVQIVSGNPCVIADAVSDVTLAFLVIRAANNFEETGNSTA
                     VLLKNSSMIYLNNVNIESGHG"
     assembly_gap    3027..3126
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3127..3531
                     /locus_tag="PL056_04165"
     CDS             <3127..3531
                     /locus_tag="PL056_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46238.1"
                     /translation="GHGSSTGSSGSAGAGGTGTGGSGGTSPYSSDRCPEPSIKGNDGN
                     SGNIGEHGAAGKSFGGYDENGYLPGHGGDGATGTHGHGGGGGGPSIGIVSIESTFDGI
                     MHTTFLVGKGGEGGKSEFNEGYPGKENMVLEY"
     gene            complement(3538..5544)
                     /locus_tag="PL056_04170"
     CDS             complement(3538..5544)
                     /locus_tag="PL056_04170"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HPM46239.1"
                     /translation="MNHSLSRSQLEAVTTTEGPVLVFAGAGSGKTRVITHRIAYLINE
                     MNVHPSSILAVTFTNKAAKEMKERVSDLIGSDETTSGLTLCTFHSLGLRILKSEFRKI
                     GYPSDFTIYSPYEQNELMKKVMVEENISSDRFSSRGLIAAVSKMKNDPSLMNDTSFLI
                     SSIVNSTARKIIGTYGRMLKTSGAMDFDDLILITVELLKNDKDVLQKYSSKYRYIMID
                     EYQDTNKTQFELIKLLSSHHGNLFVVGDDDQSIYSWRGADIENILNFEKDFKKSRVIK
                     LEENYRSVDEIVNAAGRLILNNKSRALKTCFTSKKAILDEGIKVVNILDETAEAEFTA
                     DEIRKWTAGGGKLKNIAVIIRANHQTKFFEIAFSRHAIPFSIIGGHKFFENKEIRDII
                     AYIRVVLNPFDEISLRRIINYPARGIGVSTLEKFFETASSLKINPGLFLKEFENYESL
                     FKKEQVSSIKKFVNIYKALNSEINEMEPVAFAKLLVSLSTIEDEIRKTSENETVARIK
                     LENVRAFIDAVATDPDRIKFKTARPFFSNFINSITLLQSGDEDIKTNSVTIITAHSAK
                     GLEFDYVFLAGFYQGGIPNYIAIEENNIEEERRLCYVAMTRAKKLLVITIPSTISFRG
                     STKNAEPSIFLKEAGLDEEKYTGKPPEDDPSRVFEEILKKIRNN"
     gene            6190..6792
                     /locus_tag="PL056_04175"
     CDS             6190..6792
                     /locus_tag="PL056_04175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HPM46240.1"
                     /translation="MDKNVTVVDFETTGLYPDSGDRITEIGAIKMSGNKVRGVFTSLI
                     FPERAIPESATFITGITNEMVKNAPTIGQALPLFTDFVQDDILVMHNAKFDGSFLRYE
                     MKHLSIEKEFQYFCTLIAARKDVKSSNFKLSTLKNVLGLKTFGSMHRALSDTFVTAQL
                     FLILEKNFGIDTVANMERDISELLSKMESDDLYVLKGSNN"
     gene            6845..9343
                     /locus_tag="PL056_04180"
     CDS             6845..9343
                     /locus_tag="PL056_04180"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HPM46241.1"
                     /translation="MTKNGKMAKDSIFFTDILTEESSALERDFFKALEYLQVKDRTTV
                     KERDIYLAISTAMRHRLIKDWLRTQHQYRVTKAKRVYYLSMEFLMGRLLGNMLMNIDC
                     YNEASDLVQKLGYHLEELRDLEPDMGLGNGGLGRLAACFLESMATLELPAYGYGIRYE
                     YGIFKQGIKNGYQVEVPDNWLKYGCPWEICRPELEYRVKFGGRVITVTDEKGAEKYKW
                     VDTEDVIALAYDVPVPGYDTFTVNNLRLWQARSTNEFDFQYFNSGDYMAAVNDKNVSE
                     NISKVLYPNDNMHEGKVLRLKQQYFFVSATLQDIIKHYKKNHGNSFDDFPEKVCIQLN
                     DTHPSIAIPELMRIFIDEEGIEWNDSWELTKRTFAYTNHTVLPEAVEKWDVDIMGPLL
                     PRQLQIIYEINRRFINEARDFKGFDTRRIKDVSIFEEAGHKFVRMANLAIVGSSSVNG
                     VSALHTEILKNDMFRDFNELFPGKINNKTNGITPRRWLKKANPFLANLISDQIGTEWA
                     KNLDELKKLEPLATDTSFQEVWQEAKWLAKKQLQDYVKREMNFDLNVESMFDVQVKRM
                     HEYKRQLLNVLHAITLYNRIKKNPKGDFVPRTKIFGGKAAPGYYVSKQIIKLINSVSN
                     VVNNDPEVNKYLNVFFFPNYSVSMAEKIIPASELSEQISTAGFEASGTGNMKFMLNGA
                     LTIGTLDGANVEMLEEAGADNIFIFGLTADGVKEAKMTGYNPRVIYETDPELREVLDK
                     IRSNYFNPEEPEVFAHLFNDLVTYGDNYMLLADYRSYVNAQDKVEAVYRDRFEWTKKS
                     ILNTARSGKFSSDRTISEYADEIWHVAPVKVQTD"
     gene            9354..10103
                     /locus_tag="PL056_04185"
     CDS             9354..10103
                     /locus_tag="PL056_04185"
                     /inference="COORDINATES: protein motif:HMM:NF023048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-binding motif-containing protein"
                     /protein_id="HPM46242.1"
                     /translation="MKRPLIAVAAALLTIFMDAQEVQMCTAYVDRDGDGYGTDEKTVD
                     FYCDGGVPVGFAAKKGDCNDLNRYINSGVAERCNGIDDNCDRITDPENSSECNSYYKD
                     MDGDGYGIDESKCLCVSEGKFSTLERGDCDDTNSRVFPGVKEICNDLDDNCNGIIDEG
                     EGVQGCRPFYYDSDGDGYGSGDDSKCLCKPEGLYRADMTGDCNDNNPAVHPNAHEYFD
                     RIDNNCNGIVDENPGIPGPAKNHHHKKDKKD"
     gene            10113..11048
                     /gene="nagZ"
                     /locus_tag="PL056_04190"
     CDS             10113..11048
                     /gene="nagZ"
                     /locus_tag="PL056_04190"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF003740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HPM46243.1"
                     /translation="MYPGLKTIKQKAGAPFFLSVSSSVLKSSELKMLKEINPAGIIYF
                     KRNIENCESLQYLIKSTCSVDSIKYHAVDEEGGRVRRLPAGEWSLPSMLEIAENGNLF
                     ATEKIDVLGRKLQSIGINMNMAPNVDLRSGENTSIVGDRSFGEDPQQVIQFAEIYIEM
                     MKKNGIYPVLKHFPGHGTTTVDSHKSLPVINKPLDALFEEDLLPYKALANKAGFVMVA
                     HLLHPDVSDLPATLSREWNEILRKNTGFKGISMTDDMEMHALDSYSYLEKMELFYESG
                     IDMLLVCSGNEEAISGFFEASVRMVEKGVSGKFKH"
     gene            complement(11028..11393)
                     /locus_tag="PL056_04195"
     CDS             complement(11028..11393)
                     /locus_tag="PL056_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46244.1"
                     /translation="MAKQMNELTDILKKEGFADTGCQYYREVEFNGRWRLYDVLENML
                     LAYSMIKENVELVIVVNTDCAKEIENKVTVDRNLSPVGKKFKDIAGRAKMYVVEESEG
                     RNFVTVKLKPNTFSVLKLS"
     gene            complement(11484..12260)
                     /locus_tag="PL056_04200"
     CDS             complement(11484..12260)
                     /locus_tag="PL056_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HPM46245.1"
                     /translation="MRLFFKIFFTLAITFSLFSDTLVQQPNSSVKSFSKAKRFLNKTV
                     YNGSIARKTFYCGCLYDEKLNVDLESCGYVSPKNQKRSKRVEWEHVVPASAFGKNLSE
                     WKYGHPECVDKKGKPFKGRNCASKTNKLFQLMQADMYNLVPSIGEVNGDRSDLDHGII
                     SGEKRDYGRCDFEISGKTAEPAENIRGDIARIYMYMNSAYPGFGIINEKNKKMFEAWN
                     ISDPVDTSECLRSSEIEKIQGNYNLILRKYCIELLKNKKK"
     gene            complement(12424..13854)
                     /locus_tag="PL056_04205"
     CDS             complement(12424..13854)
                     /locus_tag="PL056_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46246.1"
                     /translation="MKKMIFLVLVSGLFFISCSTSENSKNDEEVSDKGCDGFEMSDTS
                     YDEETIDNIMADSNEENDDFEENDEYSDEDSETGEFLSGFDIAGKECIADIPQGRIIL
                     TTARNYSDYDAPWRVYILILKDDGTVVDTEKYFETDPDLRGIGFNSNGRLAAVSSWKT
                     GHVTLFALIDSTLCISDTEIVLPNLKVNGEDVERVIFDEVVPHPTDPYKMILVSGNII
                     ETGDYNNYNGGIYTMTVDRFGKAVISEDRPQMHVPSAFTVLPGGNKAVVIGGKEFITE
                     EGSAVGSSGPDDLALLDLTTETPQVIKWFDIWGATGVNSSSPSVKTIGVSDNGYLMIA
                     NSSEYSDDTGKIKLFQYDGNETISHVSTFSNADLDAPDYITLNDAGTTAIVLNSLFKG
                     ATLAINDGEVSYTKKETHDLTEPMIKLKTAPFADHVLIHSFRSSNDESSSITIAEITT
                     EGLNELSSTLIPLKDDFSAQSVTVQN"
     gene            complement(13898..14245)
                     /locus_tag="PL056_04210"
     CDS             complement(13898..14245)
                     /locus_tag="PL056_04210"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HPM46247.1"
                     /translation="MSKKSDLKRLEFILTMIENIAVICERHCGIKNALNDMEGQNAIF
                     MCLVQIGEKIKKIEDLSIKEHLPVKEAASVRNFIIHDYTGVDLEIIEEIVDSDLKVLE
                     KTIKTMLKKNISR"
     gene            complement(14238..14894)
                     /locus_tag="PL056_04215"
     CDS             complement(14238..14894)
                     /locus_tag="PL056_04215"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HPM46248.1"
                     /translation="MRESAISARIDFEKKKRVEEVFAKLGLTHSSAINLFYSQVLLHG
                     GLPFETRLKESSGMTVAEPSVAYNQTGKEIMTLLSKFGKSKEEKAEIVKLALEYFLNE
                     VAGRDDSSKESVAYDRKTILKKLRQIKKKYEKNGFVILGIFGSYARNEENESSDLDIL
                     YELSSDFRAKFKGFKAIAEINRIKEELENHFGMKIDLVDRSTQNSSEHNTILDEAVYV
                     "
     gene            complement(14979..15389)
                     /locus_tag="PL056_04220"
     CDS             complement(14979..15389)
                     /locus_tag="PL056_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46249.1"
                     /translation="MRYDILIAIYSFAFVMTIVLELPVVWFLSHKVFKHPLKKTIISG
                     LLSQIATHPLFIGVIPVAVILLKNDAPEWFKFIDNIYLKELLLIPLIEAGIYYAILKP
                     AEKWQPFAISYAANLTSWGIGYLIPMKWIIELMR"
BASE COUNT         4589 a         2921 c         3394 g         4483 t
ORIGIN      
        1 tcagctctca tcttgcttta aattcagttg taatgtataa ttaagaccat tatgtgcttt
       61 gatcttttcg ggggtttaaa tgaaaaagtt ctcggttttc ttaattgtca ttgcagttgt
      121 tatatttact tcctgtgata agaacaaggg agatgatttc acttacggtg attatcccga
      181 ttctgtaaat aaaaacgatg aagatgatta taactatgtt aaaaaagatg cggatactgt
      241 caaaaaagac gacgacagtt atacatcaac cgataatgac actgtgtctg atcccgatga
      301 tgatacattt attctgaccg atgaagataa tttcacactt cctgatgatg acaattttac
      361 cttgccggat gaagacggtt ctgttctggc tgacgaagac aatttttctt ttcctgatga
      421 agataactat attctgcccg atgaggataa ttccacactt cctgatgaag attcaactgt
      481 cacacccgac aatgctgtgg ctgatataga cacttatgtc gagccggaat gcaagaacgg
      541 tatcgttgaa gatggagaaa tatgcgaacc gacaattccc aaacagtgtc acacattgaa
      601 tcccgaaata tatcagagcg gctgggcaac ctgtgaagat gactgttccg gctggataat
      661 gacatcgtgt gttgaatatc catgctctga aaaacccgat gttcctgact ctttattcac
      721 cgactcaaac tgtgacggaa tagacggtaa cataatagaa tcagtattcg tagaccctgt
      781 cttcggatcg aacatgaatg acggaacgat gtcgctgccg gtcgcaacaa ttacaaaagg
      841 gattgagata gcacaaaaca cagggaaaaa gcatgtgctt gtttcaggag caagttatag
      901 cgagaatgtg gtgcttaaaa caggaatatc agttcacggc ggctacagcg gaaatccgaa
      961 ctggacaaga agcaccatgt ttgaaactat tattcagaca ggggtaaacg gaattcttgc
     1021 agaatgggtt ggtgatttta ccttatcgtt cctgacgatc aggagtgcaa atgcagtttc
     1081 agcgggacaa agctctttcg gcatggtttt gaaaaactgt gaaaatataa ctctcagcaa
     1141 tgtaaaagtc attgcaggga acggtgcccc cggaacagat ggaacggacg gagttaaagg
     1201 gggagacgga actcagggaa cgcaggggaa tcagggttgt gaaagcagtg gcggattttt
     1261 ctgtgacagc ccttgctcag ctccggaagg cggtgcaggc ggaagttcac catgcggagc
     1321 aaacggaggt aaagggggca caccgggaaa aggaagttcg aacggatatc cgggagagaa
     1381 cggagtagga tcaactgata acggcggaga tggtgatatt gcaggaagct gggacggaat
     1441 gtgcctttac tatgaaggtt tggcagtcca cggaggcgat ggagagctcg gatctttcgg
     1501 tgttaacggg actggcgggg ggactctcgg tacagtatct gaatcaggat atgtgccggc
     1561 agacggaggc aacggaactg acggtgagca cggtcagggt ggcggcggcg gcggcggcgg
     1621 ttgtggcgga actgatgcat gtaacagtta cggttcttca ggcggtggcg gcggcggagg
     1681 cggttgcggc ggaacagcag ggacaaaagg gaccggcgga ggaggaagtt tcgcattatg
     1741 gctttataag tgcacagaca tagtttttga gacagtccgg cttgaatcaa agaacggcgg
     1801 agcaggcgga aaagggggaa acggtatgtc cggaggattt ggagcacttg gcggagcagg
     1861 cggcacatac ggtggtgacg gagatcagga tgacggcggt tgcggtggct ggggaggcgc
     1921 aggtggaagc ggcggaacag gcggaagcgg cggcggcggc ggcggcggac cgagcattcc
     1981 aatggtaaag atacaaagtt cagttgaggg aatgtatttt gccacatatg tctttggaag
     2041 cggcggtgca ggcggaccga gcaacggaaa tgtcggatta aacggcaggg caggacaata
     2101 ttatgaagat taaatttcat attggggaga gatttttgaa aaataccagt attctttttc
     2161 ttgttgtttt ttttgttttt gcatgtaccc cggataaaga cgattcaata tatgactatc
     2221 ctgataatac agttgtaaaa aatgacgagg acagttcagt aaaaaatgat aatacggtca
     2281 taaatgattc tgattcacaa ctccctgata cgcatgtttt accggacggt gaacagccgg
     2341 acacattgac tcccgacaat gaatcgaccg acgatccctt tattgcgaca tgcggaaatg
     2401 gaattgtgga ttttggagaa gtctgtgaaa gggatacaga aaaggagtgt tccgcaattg
     2461 atccaggttc ttatgtagac ggtgttgcaa aatgtaatga ttcatgcagc ggatggatag
     2521 tttctgactg cgtttcagtc ggttgtgaaa cagcggttga caatcccgat gatgcttttg
     2581 cagacacgaa ttgtgacgga atagacggag atgcatctgc ttcggtattt gtagataaac
     2641 ttaacggtct gaacactaat cccggaacaa aggatctacc ggttgcgaca attatgaaag
     2701 cacttgaaat tgcacaggag caggacaaaa agcacatcct tgtcggcaca ggggcttatt
     2761 ctgaacaggt tgaacttaaa gaaggaatat caatccacgg tggctacagc ggtcatccta
     2821 actgggcaag gggagttgaa tatgaagtgc agatcgtgag cggcaatcca tgtgtcatag
     2881 ccgatgcagt ttcagatgtc actcttgcat ttcttgttat ccgtgccgca aacaattttg
     2941 aggaaaccgg aaattcgacg gcagtgttac ttaagaacag ttcgatgatc tatctgaata
     3001 atgtaaatat tgagtcagga cacggtnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnggac acggttcatc aacgggatct tccggttcag caggagcagg gggaacggga
     3181 acaggcggaa gcggcggcac aagcccgtat tcaagtgaca gatgtccgga gccgagcata
     3241 aaaggtaatg acggaaattc cggaaatatt ggtgaacacg gtgctgcagg gaaatcattc
     3301 ggcggatatg acgaaaacgg atatcttccc ggtcacggag gagacggtgc aacagggact
     3361 cacggtcacg gtggcggcgg cggcggaccg agcatcggta tcgtaagcat agaaagcaca
     3421 tttgacggaa taatgcacac cactttcctg gtcggtaaag ggggcgaagg gggtaaaagc
     3481 gaatttaacg aaggataccc cggaaaagaa aatatggttc ttgaatatta aataaattca
     3541 gttatttcga atcttcttca aaatttcttc aaacactctc gaagggtcgt cttcaggtgg
     3601 tttccctgta tatttttctt catcaagccc cgcttcttta agaaaaatag agggttctgc
     3661 gttttttgta gatcccctga atgaaatggt tgaaggtatt gtaattacaa gcagtttttt
     3721 tgctcttgtc attgcgacat agcacaacct tctctcttct tcgatattgt tctcctctat
     3781 cgcaatgtag ttggggattc ccccctgata gaatcctgca aggaaaacat aatcaaattc
     3841 aagccctttt gcgctgtgag ctgtaattat cgtgaccgaa ttggtcttta tatcttcatc
     3901 gcctgactga agaagtgtta ttgagtttat gaaatttgaa aagaagggcc ttgccgtttt
     3961 aaatttaatt ctgtcgggat ctgttgccac tgcatcgatg aaagcgcgca cattttcaag
     4021 ttttatcctt gccacagttt cattttcaga tgttttcctt atttcatctt caatggttga
     4081 cagcgacacg agaagttttg caaaagcaac cggctccatt tcatttattt cgctgtttaa
     4141 cgccttgtat atattaacaa attttttaat tgaagaaacc tgctcctttt taaataatga
     4201 ttcatagttt tcaaactctt taagaaaaag tccgggattt atctttaatg atgaagctgt
     4261 ttcgaagaat ttttcaagag ttgaaactcc tattcctctt gccggatagt tgattattct
     4321 tcttaaactt atttcgtcaa aaggattgag aacaactctg atgtatgcga tgatatcccg
     4381 tatttccttg ttttcaaaga acttatggcc tccgattata gaaaacggga tggcgtgacg
     4441 gctgaaagct atttcaaaga acttggtttg gtggttagcc cttataatta ccgctatgtt
     4501 ctttagcttt cccccccctg cggtccattt tcttatttca tccgcagtga actcggcctc
     4561 ggcagtttca tcaaggatat taactacttt tatcccttca tccaggatcg cttttttaga
     4621 agtgaaacag gtttttagag ctcttgactt attgttgagg atcaaccgtc cagcggcatt
     4681 tacaatttca tctacagatc tgtaattttc ctcaagtttt ataaccctgc tttttttaaa
     4741 atccttttca aaattcaaaa tattctcaat atcggcaccc ctccagctgt aaatgctctg
     4801 atcatcatca ccgacaacaa aaagatttcc atgatgagat gaaagaagct taatcaattc
     4861 aaactgggtc ttgttggtgt cctgatattc gtcgatcatt atatatctgt atttacttga
     4921 atatttctgc agaacatctt tgtcgttctt tagtagttct acggttataa ggatcagatc
     4981 atcaaaatcc attgcacctg aagttttcag catccttcca taagttccga tgatctttct
     5041 ggcagttgaa ttgacaatgc ttgatataag aaaagatgta tcgttcataa gtgatggatc
     5101 gtttttcatc tttgagacgg cggcaattag tccccttgaa gaaaatctgt cgcttgaaat
     5161 gttctcctct accatcactt ttttcatgag ctcattttgt tcatatggtg aatatatagt
     5221 aaaatcagaa ggatacccga ttttcctaaa ttcacttttt aatattcgca gccccagaga
     5281 atgaaaggtg caaagcgtta agccagatgt tgtttcgtca gaaccgataa gatccgaaac
     5341 tctctctttc atttcttttg cggccttgtt tgtgaaagta actgcaagaa ttgatgacgg
     5401 atgaacattc atttcattga taaggtaggc aatacggtga gtaattacac gggtttttcc
     5461 tgatccggca cctgcaaaca caaggacggg tccttcggtt gttgtaacgg cttcaagttg
     5521 tgagcggctt aaagaatggt tcaatcttca ccccaaaaaa agagagtttt gcaactgaca
     5581 gtaataatat ggcttttttt aacatttttt caacattatt ggaaattatt tcatatgatg
     5641 cctgttttca acactaaatc atcaagagat tagagtaagt ccttaataac aatgaggaaa
     5701 gctgttgcac tgaaaagtgt gcaagtggtg gaagtatttg attatcaaga ttttttctgc
     5761 aataaagagc aaaagtgtta acatttatgt tgaaaacccg aagttaaaga gagctccgtt
     5821 atgtgaggat aaaaaaattc ccggatttca aaaaatgata caactttttt tgagggtctc
     5881 agggagattt ataaacactt ttttaacata tgcatcaaat gtgttaatta atgctgtctt
     5941 tgaacattaa aagatgtgta aatgattgca agatcttgaa attatatgat aaaatggatg
     6001 cacataaaaa tgtgcaaaac agaaaaaacc atgtaattca agaggttctt ccatataaaa
     6061 aggagaaaat tcaacataaa tgttaaaaag atgccggtat ggctgaagga aaagtttgac
     6121 taaataaaac accattataa agttttaccg gttgccggat cgttttaaca caaaaaaaca
     6181 ggtgcctgaa tggataagaa tgtaacagtt gtagattttg aaactaccgg gctgtatccc
     6241 gattcagggg acaggataac cgagattggt gcgataaaaa tgtcgggcaa caaggtgaga
     6301 ggagttttca cttcgctcat ttttcccgaa agagcgatcc ctgaatcagc gacatttatt
     6361 acaggtataa cgaatgaaat ggtaaaaaac gctccgacga tcggtcaggc acttcctctt
     6421 tttacagatt ttgttcagga tgacattctt gtaatgcaca atgctaaatt tgacgggtct
     6481 tttttgagat atgaaatgaa acatctgagt attgaaaagg aattccagta tttctgtaca
     6541 cttatagccg caagaaaaga tgtcaaatct tcaaatttca aattgtcgac acttaaaaat
     6601 gtgctggggc ttaaaacttt cggctcgatg cacagagcac tttcagacac atttgtgaca
     6661 gcacagcttt ttctgatact tgaaaagaat ttcggaatag ataccgttgc aaatatggaa
     6721 agggacatca gcgaactcct ttcaaaaatg gaaagtgacg atctttatgt tttgaaaggt
     6781 tcaaataatt gaaaaataac tgcactgtgt taaaatcatc cgttttattt atcgggagtg
     6841 aaaaatgaca aagaatggaa aaatggctaa ggattcaata ttcttcacgg atatcctgac
     6901 ggaagagtcg agtgcattgg aaagagattt tttcaaggcg cttgaatatc ttcaggttaa
     6961 ggaccgtacg acggtgaagg agcgtgatat ttatctggcg atatcaacgg caatgagaca
     7021 ccggctgatt aaagactggc tgagaaccca gcatcagtac agggttacca aagcgaaaag
     7081 agtttattat ctttcaatgg aatttcttat gggccgtctt cttggaaata tgctcatgaa
     7141 cattgactgt tataatgagg catccgatct tgtccagaaa ctgggatatc atctggagga
     7201 gcttcgtgac cttgagccgg acatggggct ggggaacggt ggtctgggac ggcttgccgc
     7261 ctgttttctt gaatcgatgg ctacacttga gctgccggct tacgggtacg gaataagata
     7321 cgaatacgga atattcaagc aggggatcaa gaacggttat caggtggaag tgcctgacaa
     7381 ctggctgaaa tacggttgtc cgtgggaaat atgcaggcct gaactcgaat acagggtcaa
     7441 gttcggaggc agagttataa cggtaactga tgagaaaggt gctgaaaaat ataagtgggt
     7501 cgatacagag gatgtcattg cgcttgcgta tgatgttccg gttcccggtt atgatacttt
     7561 tacggtaaat aatctccggc tctggcaggc aagaagtaca aatgagtttg attttcagta
     7621 tttcaacagc ggtgattaca tggccgcagt aaatgataaa aatgtaagcg agaacatttc
     7681 aaaggtgctt taccctaacg ataatatgca tgaaggaaaa gtgcttcgtc ttaaacagca
     7741 gtatttcttt gtgtcagcaa cacttcagga cataattaag cattacaaga aaaatcacgg
     7801 aaactcgttt gatgatttcc ctgaaaaggt gtgcatacag cttaacgata ctcatccgag
     7861 cattgccatt cctgaactga tgaggatatt tatcgatgaa gaagggatag agtggaatga
     7921 ttcatgggaa cttacaaaga gaacttttgc atatacaaac cacaccgttc ttcctgaggc
     7981 ggttgaaaaa tgggatgtcg acatcatggg acctcttctt ccaagacagc ttcagataat
     8041 ttacgaaata aacagaaggt tcatcaacga agccagggat ttcaagggct ttgacacaag
     8101 aagaataaaa gatgtctcga tttttgaaga ggcggggcac aagttcgtaa gaatggccaa
     8161 ccttgcaatt gtcggaagca gttcagtaaa cggtgtgagc gcccttcata cagagatcct
     8221 taagaacgat atgttcaggg atttcaatga gcttttccca ggcaagatca acaataaaac
     8281 caacggtata actccgagaa ggtggcttaa gaaggcaaat ccgttcctgg cgaatctaat
     8341 ttcagatcaa ataggaacag aatgggcaaa gaaccttgat gaacttaaaa aacttgaacc
     8401 gcttgccacc gacacatcat ttcaggaagt gtggcaggag gcaaaatggc ttgccaaaaa
     8461 acagttgcag gattatgtga aaagagaaat gaactttgac cttaatgtgg aatcgatgtt
     8521 cgacgttcaa gtcaaaagga tgcacgaata caaaagacag cttctcaatg ttcttcatgc
     8581 aataacactt tacaacagga tcaaaaagaa ccccaaaggt gattttgttc caaggacaaa
     8641 gatattcggc ggaaaggcgg ctccgggata ttatgtttca aagcagataa tcaagctgat
     8701 aaattcagtt tcaaatgttg taaacaatga tcctgaagtg aataagtatc tgaatgtatt
     8761 ctttttcccc aattattcag tgtcaatggc cgaaaagatc attcccgcaa gcgagctttc
     8821 tgaacagata tcaacggcag gttttgaggc aagcggaaca ggcaacatga agttcatgct
     8881 taacggagca cttactatag gaactctaga cggagcgaat gttgaaatgc ttgaagaagc
     8941 aggtgctgac aacattttta ttttcggtct tactgcagat ggtgtaaaag aggcgaaaat
     9001 gacgggatat aatcccagag tgatttatga aactgatcct gaactgagag aagtgcttga
     9061 taagattaga agtaattact tcaaccctga agagccggaa gtttttgcgc atcttttcaa
     9121 cgatcttgtt acctatggcg ataactacat gcttcttgct gattacaggt cgtatgtcaa
     9181 cgctcaggat aaagtcgaag cggtttacag ggacaggttc gaatggacaa agaagtcaat
     9241 acttaatacc gcaagaagcg gaaagttctc ttcagacaga acgatcagtg aatatgctga
     9301 tgagatctgg catgttgctc cggtgaaagt gcagacagat taaggaagtt gcgatgaaac
     9361 gcccgttgat tgcagttgcc gcggcactgc ttacaatatt tatggatgca caggaagttc
     9421 agatgtgcac ggcttatgtt gacagggacg gagatggata cggaacagat gaaaagacag
     9481 ttgattttta ctgtgatgga ggtgttcctg tcgggtttgc ggcaaaaaaa ggggattgca
     9541 acgatctcaa cagatatata aattcgggag ttgcagaaag atgcaacggc attgatgaca
     9601 actgtgacag aataacagat ccggaaaatt caagcgaatg caacagttac tacaaagata
     9661 tggacggaga cggttacggg attgacgaat cgaagtgtct gtgtgtatct gaagggaaat
     9721 tctcaacact tgaaagaggc gactgtgatg acacgaacag cagggttttt ccgggtgtta
     9781 aagagatttg caacgatctg gatgataatt gtaacggcat tatagatgaa ggagagggtg
     9841 tccagggatg cagaccgttc tattatgact ctgacggaga cggttacgga tccggagatg
     9901 attcaaagtg cctttgcaaa cccgaaggtc tttacagggc agatatgaca ggggattgta
     9961 atgacaataa tcctgctgta cacccgaatg cacacgaata ttttgaccgc atagataaca
    10021 actgtaacgg aatcgtcgat gaaaatccgg ggatacccgg tccggctaag aaccatcatc
    10081 acaaaaaaga taaaaaagat taaggagttt tcttgtatcc gggactgaaa acaattaaac
    10141 agaaagccgg cgcccctttt tttctgtctg tctcatcatc agtgcttaaa agttcagaac
    10201 tgaaaatgct taaagagatc aatccggcag gaattatata ttttaaaaga aatatagaaa
    10261 actgtgaatc attgcagtat cttataaaat caacatgcag tgtggactcc ataaaatatc
    10321 atgcagttga tgaagaggga ggcagagtcc gccgtcttcc ggcaggtgag tggtcacttc
    10381 cttcgatgct tgaaatagct gagaacggaa acctttttgc gacggaaaag atagatgttc
    10441 tcggaaggaa acttcaaagc atcggaataa acatgaacat ggcgccgaat gttgatctca
    10501 gaagcggaga gaatacttca atcgtcggtg acagatcttt cggtgaagat cctcagcagg
    10561 ttatacagtt tgctgaaatt tatatagaaa tgatgaaaaa gaacgggatt tacccggttt
    10621 taaagcattt tcccggtcac ggaaccacaa cagtagattc gcacaagtcg ctgccggtga
    10681 taaataaacc gctggacgcc ctttttgaag aagatcttct gccgtacaaa gctcttgcaa
    10741 acaaggcagg gtttgtgatg gtcgcccatc tgcttcatcc tgatgtgagt gatcttcctg
    10801 cgactctttc aagagaatgg aacgaaatac tgaggaaaaa cacgggattt aaagggatct
    10861 ccatgaccga tgatatggaa atgcatgcac ttgacagcta ttcctatctt gaaaaaatgg
    10921 aactgtttta tgaaagcgga atagatatgc ttcttgtctg ttcaggtaat gaagaggcaa
    10981 tatcaggttt ttttgaagcg tccgtcagga tggtcgaaaa aggagtttca ggaaagttta
    11041 agcactgaaa atgtattcgg tttaagcttt accgtaacaa aatttctccc ctctgattct
    11101 tcaacgacat acatttttgc tcttcctgcg atgtctttga attttttacc gacaggggaa
    11161 agatttctgt caacggtcac tttgttctct atttcttttg cacagtcagt atttacaaca
    11221 atgacaagtt caacattctc tttgatcatg gaatatgcaa gaagcatatt ttcaagaaca
    11281 tcgtaaagac gccatctgcc gttgaattca acttcgcgat aatactggca tcctgtgtct
    11341 gcaaaacctt ctttttttaa aatgtctgtg agttcattca tctgcttagc catttttccc
    11401 tcctttaata agatggcatt gaggatttta agtaaatcga tcctaaaatc aaccagaaaa
    11461 acattttttt tgaacactcc ggttcatttt ttcttgtttt tcaggagttc aatgcaatat
    11521 tttcttaaga taaggttgta atttccttga attttttcaa tttcagagct tctaagacac
    11581 tctgatgtgt caacggggtc tgatatgttc catgcttcaa acattttttt gtttttttcg
    11641 ttgattatgc caaaaccggg gtacgctgaa ttcatataca tataaattct tgcgatgtct
    11701 ccgcggatgt tttcagcagg ttctgcggtt ttgcccgata tttcaaaatc acatctgccg
    11761 tagtcccttt tttcacctga aattataccg tgatcaaggt ctgatctgtc accgttcact
    11821 tcacctattg aaggaacaag attatacata tcggcctgca tcaactggaa aagtttgttt
    11881 gtttttgaag cgcagtttct ccctttgaac ggttttcctt ttttgtcaac gcactccgga
    11941 tgtccgtatt tccattcaga aaggttcttt ccgaatgccg aggcagggac gacatgttcc
    12001 cattcaactc ttttcgacct tttttggttt ttcggtgaaa catatccgca gctttcaagg
    12061 tcgacattaa gcttttcatc ataaagacat ccgcagtaaa atgtttttct ggcaatactt
    12121 ccgttgtaaa cggtcttgtt tagaaacctc ttcgctttgg aaaaagattt tactgaggag
    12181 ttcggctgct ggaccaaagt atctgaaaac agtgaaaaag ttatcgctaa agtaaaaaat
    12241 attttaaaaa ataaacgcat cgccacctcg cgaatttata cctatcttaa tttattatcc
    12301 gatgttaata aacagaaatg tcagcgaata tagagtgtta tatgtttagc ggcgtaatga
    12361 aaataatagc ttgaccgttg gtgattcaaa atcggttgtt agaaaattga agacgatcga
    12421 tacttagttc tgaacagtca cgctctgggc tgaaaaatca tctttaagcg ggatcagagt
    12481 tgatgataat tcgttaagac cttcagtcgt tatctctgca attgtgatgg atgaactttc
    12541 gtcatttgaa cttctgaatg aatgtatgag tacatggtcg gcaaatggag ctgttttaag
    12601 ttttatcatc ggttccgtga ggtcgtgtgt ctcttttttt gtgtaagaca cctccccatc
    12661 attaattgca agtgttgccc ctttaaacaa tgaattcagt actatagctg ttgttccggc
    12721 atcgtttaat gtgatatagt cgggagcatc aaggtcggca ttagaaaatg tcgaaacatg
    12781 tgaaatcgtt tcattcccgt catattggaa aagctttatt ttgccggtgt catctgaata
    12841 ttcgcttgaa ttggcgatca ttaaatagcc gttatcagaa actccgattg tttttacaga
    12901 tggacttgaa ctgttaactc ctgttgctcc ccagatatca aaccatttga taacctgcgg
    12961 agtttcagtt gtgagatcta aaagtgcaag atcgtcaggg cctgatgaac cgactgcact
    13021 cccttcttca gttatgaact cttttccgcc gataacgact gccttatttc cacccggaag
    13081 aactgtaaaa gcacttggaa catgcatctg cggacggtct tcgctgatga ctgctttccc
    13141 gaatctgtca acagtcattg tgtaaattcc gccgttatag ttattgtaat cacctgtttc
    13201 aatgatgttt ccgcttacaa ggatcatttt gtaagggtca gtcgggtggg ggacaacttc
    13261 atcaaaaata acccgttcaa catcttcacc attcactttt aaattcggca gaacaatttc
    13321 agtgtcagaa atgcagagcg ttgagtcaat taacgcaaaa agagtgacat gccctgtttt
    13381 ccatgaagaa actgccgcaa gacgcccgtt tgaattgaat ccaatccctc taagatcagg
    13441 atctgtttca aaatattttt cagtgtcaac aacagtcccg tcatctttta gaatcaggat
    13501 ataaactctc catggtgcat cataatcaga ataatttctt gcagttgtca gaataatccg
    13561 accttgcgga atgtcagcaa tacactcctt gcctgctatg tcaaaacctg aaagaaattc
    13621 accggtttca gaatcttcat cagaatattc atcgttttct tcgaagtcat cgttttcttc
    13681 atttgaatct gccataatgt tatcgattgt ctcttcatca tagctggtgt cagacatttc
    13741 aaacccgtca caacctttgt cactcacttc ttcatcgttt ttactgtttt cagaagttga
    13801 acatgaaata aaaaacaacc ccgaaaccag aactaaaaaa atcatttttt tcataatatt
    13861 ctccattttt gtcaccgcat cagttcaatt atccatttca tcgggatata ttctttttca
    13921 acatcgtttt aatagttttt tcaagcactt tgaggtcgga atcgactatt tcttcaatta
    13981 tttcaagatc gactccggtg tagtcatgga taatgaaatt tctcacactt gccgcttctt
    14041 tgacaggcag atgttctttg attgacaaat cctcgatttt cttaattttt tcacctatct
    14101 gaaccagaca cataaaaatt gcgttctgtc cttccatatc attaagagca tttttaattc
    14161 cacaatgcct ttcacatatt acagctatgt tttcgatcat ggtgagtatg aattcaagcc
    14221 gtttgaggtc gcttttttta gacatatact gcctcatcga ggatcgtgtt gtgttcagag
    14281 ctattttgtg ttgatctgtc aacgaggtca attttcatgc cgaaatgatt ttcaagctct
    14341 tcttttatcc tgtttatttc agcaatggct ttaaatcctt tgaattttgc tctgaaatct
    14401 gatgaaagct catataaaat gtcaagatcg cttgactcat tttcttcgtt tcttgcatat
    14461 gaaccgaata ttccaagaat tacaaatccg tttttttcat acttcttctt gatctgtcta
    14521 agtttcttta aaatagtttt tctgtcataa gcgactgatt cttttgaaga atcatctctt
    14581 ccggcaactt cattaagaaa atattccaga gcaagtttta caatttctgc tttttcttcc
    14641 ttcgattttc caaactttga aagcaatgtc ataatttctt tacctgtctg attatatgcg
    14701 acagatggtt ctgcaacggt catccccgaa gattctttta atctcgtctc aaaaggaagt
    14761 cctccgtgaa gaagaacctg actgtaaaaa agatttattg cacttgaatg agttagaccg
    14821 agtttggcga aaacttcttc gactcttttc tttttttcaa agtcaattct tgctgaaatt
    14881 gccgactctc tcataatatt tttctccaat actttcacac ctatattgta atacatagaa
    14941 atacattgtc aagaggtttc ccccaaacca tataaaaatc accgcatcag ttcaattatc
    15001 catttcatcg ggataagata gccgattccc cagcttgtga gatttgcggc ataagagatg
    15061 gcaaacggct gccatttttc tgccggtttt aaaattgcgt aataaattcc ggcttctatc
    15121 aaaggaatca aaagcaattc cttcagataa atattgtcaa tgaatttaaa ccattccgga
    15181 gcgtcatttt tcagcagaat gacagcaaca ggaataaccc cgatgaacaa cggatgtgtc
    15241 gctatctgac tcagcagacc tgaaatgatc gtctttttca gcggatgttt aaaaacctta
    15301 tgcgacagaa accagacaac aggcagttcc agcacaattg tcatcacaaa cgcaaatgaa
    15361 tatatcgcaa tcagaatgtc ataccgcaag acctactcct tttaaaatca gattttagtc
    15421 aaactgcagc catccgaatc agttttatta gaagtgtctg atgtttcttc cggcttgtta
    15481 tcaatgt
//
LOCUS       DAOOYB010000045        15372 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_3836_length_15372_cov_8.118822, whole genome shotgun sequence.
ACCESSION   DAOOYB010000045 DAOOYB010000000
VERSION     DAOOYB010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 15372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15372
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3836_length_15372_cov_8.118822"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="PL056_04225"
     CDS             <1..312
                     /locus_tag="PL056_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016776777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HPM46250.1"
                     /translation="LFVFTGIFWMTDPNHYADPLFHMLSGGVMLGAWFMATDMVTSPM
                     SVKGQILFAVLAGVLCGSIRLFGGYPEGCSYSILIMNAFVPLIDKYIKPVRFGKEVNY
                     G"
     gene            305..874
                     /locus_tag="PL056_04230"
     CDS             305..874
                     /locus_tag="PL056_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HPM46251.1"
                     /translation="MAKKLESTFPNMVLVLTVISIVAAAALGFTYTKTKTQIAEMQKQ
                     KKISAIEKVVPKFNNNPDSEKYTVKELAGLEFYPAKMDGVLVGTAVQTFTEKGYAGRI
                     DIMVGFNDKMDFVDYVILNAKETPGLGTKLSDNKFKSQFPGKNPATFNLKVKKDGGDV
                     DAITAATISSRAFLDAVNKAVEGLKKGGE"
     gene            874..1464
                     /locus_tag="PL056_04235"
     CDS             874..1464
                     /locus_tag="PL056_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HPM46252.1"
                     /translation="MSLFKEFSKGIIKENPTLVLLIGMCPPLAVTSSAINGMGMGIAT
                     TFVLVCSNLVIAALKNIIPDKVRLPAFIVVIASFVTIVDLSMQAYTPALSASLGVFIP
                     LIVVNCIILGRAEAFAQKNGIIPSIFDGLGMGLGFTLALTILGGIREVLGNGSIFDIK
                     FVSEDATTILLFVLAPGAFIMLGLMIAAMNKLMKKV"
     gene            1475..2050
                     /locus_tag="PL056_04240"
     CDS             1475..2050
                     /locus_tag="PL056_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit A"
                     /protein_id="HPM46253.1"
                     /translation="MDFYMKIMIGSILVNNIILAQFLGICPFLGVSNKVSTSVGMSAA
                     VIFVMTLATTVTYLLYSRVLVPLHIEFMQTVGYILVIAALVQMVEIIMKKVSPPLYQA
                     LGVFLPLITTNCAVLGVAIIVIQKQFNLVTSVVFTIATALGFFLALVVFAGIREQLEM
                     FEPPKAMKGAPLSLITAGLLSLAFMGFAGLV"
     gene            2200..3069
                     /locus_tag="PL056_04245"
     CDS             2200..3069
                     /locus_tag="PL056_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46254.1"
                     /translation="MVDLKDLGTMENEMIALVSSGVVYTFADIQKLYNENRNKFPAYN
                     RSFKKFLRKLLELGLAERHILDEEGYQKKTVFTFGDFDSYECISKINKNAYFSHHTAL
                     FMNSMTEQIPKTAYVSYELSQTKITVTRPELKQENIDKVFSAPPREARYYLFNRRQVI
                     LHASKFNGYTGVIKNSNGTFFQTDPERSLIDSVVRPYFSGGIFNVLNVFIENRERYSI
                     SKIVNYLKALNYIYPYHQAIGFLLEKAGFPEKDLALFENMGIKHKFYIDYEISDRSLS
                     KRWNLIYPSAFDE"
     gene            complement(3041..3928)
                     /locus_tag="PL056_04250"
     CDS             complement(3041..3928)
                     /locus_tag="PL056_04250"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HPM46255.1"
                     /translation="MPNAKLSEAWLLDMRKKIMLSFFSDDELMDIFVLKGGNALELIY
                     KVTKRPSLDIDLSMEDDFVESDLRRIEKKIFKSLNDFFKDDNLEVVELKFEEKPAPEK
                     RNTPRGNGYKITFKLIEVKEAFIGESEDIKRKRALDIAGLKKTFHADISKYEYCESKV
                     KVNVDGYTVYVYSPEMIILEKMRAICQQTKEYRSQENSGGRPRPKDFYDIYMLINKMK
                     INIRSAENALMLKKIFEKKRVPADLLLIIKEYREYHRNGFRELLDTLPEREKNVSYNE
                     IFDYVVEKMLSIHQTLKDI"
     gene            complement(4425..>5761)
                     /locus_tag="PL056_04255"
     CDS             complement(4425..>5761)
                     /locus_tag="PL056_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46256.1"
                     /translation="MKRFLAITILFSFVLVLSAQETKKEPVEQKKTEVTEQKKESVEQ
                     KKLLVQKVEAAGVEEAFAKSLEEALVLDIGGREGISVVTSAEMQNVVKTAQLKSETGC
                     EGSDECLIEVQKKLDVETLIAGKVTKLGDEYILSINTVNVANKTVGERISVQGKDLND
                     VKGKIKESVDILLGISAPKQMFKLADGEELRLAVMPLAARGMEQTTADSLTSILSAQL
                     NQIKGISVISQDDIKAMLSKAALDSSVSCTDSLECVVEIGASLGLSKLVTGAVGKVKD
                     TFVISVQLIDVRNADVVNRVLESYTGDEGELQNAIKLAAYQIAGVDYKALLGEVDFSF
                     NVKKGTIQFGETESPFEKPQYSAGNLIPGRYYLKVRAANDNYLPLQTDVYVAPGAKNV
                     KTFNVLEKPTKWYKSWWFWTITSVVVLGAAGATTAVILTQQTPSGQGTVTVE"
     assembly_gap    5762..5861
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5862..6166)
                     /locus_tag="PL056_04260"
     CDS             complement(<5862..6166)
                     /locus_tag="PL056_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013409647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HPM46257.1"
                     /translation="MKILRIPFVLIISVIAFSILFADEPKSEGLKKGDKMVDFGMRKV
                     DVEKGKLSSLVWLGDYVGKKDSKEKKKILVLNFFANWCAPCIKEMPVLQGLYEKYSE"
     gene            complement(6170..8056)
                     /locus_tag="PL056_04265"
     CDS             complement(6170..8056)
                     /locus_tag="PL056_04265"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HPM46258.1"
                     /translation="MKSSPYLKLLVVVAAFLVGTAVVHTPAVKKLDFSFLDLMMISVE
                     PEAPAESVLIAVDQYSLSVFGWPVDRGLYGAMISVLTEAGAKKIGFDLFFTDRKEETD
                     TEALKRMVESEEVLGEESSKAGAILGSFVNTDYRKDPSKVVEYPPTPENSGINCPCSD
                     LSEEEIEESKFKFLEKMVPGVLKFNPLVAHVHVIPSDVDRVNRTIIGCKKVFDGCVPD
                     LALAMVQKGPYEGCCKEEIIPYFRTSSEFTVIPMVDVIESSGSDEKMKILREKVNGKY
                     VFVGATDETLKDIGPTPSGLVEPLVFLHLNRAEALLNDIRITRTSLWIIILIPFLILL
                     ASAVLFEHARAFLISGTVWVAVIYSASYVLFRKSFIFIPPMEMFIPFFFANIACAGYL
                     GWKYFLFNQVLSNAFDNYVSPEILSWLKETGGKVLQSNSAERRQITILFSDIAGYTSL
                     SNSLNAESIMKSLRLYLDEMMTITAEHNGYVDKINGDGLMILFGAPKPFGDHAKKALD
                     CAVAMQKKVHDMQKEWMEITGRELKIRIGIATDTVFVGNLGGAGHIEYSAIGRGVNFA
                     ARLESKSEIGGILVSEETVESAGLRPEGYKRSVELKGYEGEVAVWQISPVNYEKEKIN
                     ENKE"
     gene            complement(8060..10864)
                     /locus_tag="PL056_04270"
     CDS             complement(8060..10864)
                     /locus_tag="PL056_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46259.1"
                     /translation="MRNFFSMVTVVLMMFCYFSIQAVDLRIVLLPKMTVDGKEYDSAS
                     IETEMASILTGKNMRIVELSTALKAQKAALSDLVAEGKVPTELSVLNADALVSAQLAC
                     DKNSDSIMGSNIKSWFCVLNSKVIRVDSGDVIFADSKNYTATGLNALQAINTVVKKNM
                     RKNADAAVDQWSKNWSNEGKWNMDIIITGVAQKTKIDQINGELKKVDGVKDAAVVMFK
                     KDLSKISVSGEGAANYSKLREAIDTSDKLSLRVNYEAGRVIHAEFDFGQAYSRGVAAY
                     ITPPKSGGKKTMADLIASKGPDLLTSYLMNLEYFEIKESETLKGSKDSAVSEAKKNDV
                     PLVVFAEVVEKDDEWLSVIDLITSKDGKKIAVAKGTAGDPFEAMDKAVRTMDATYRIS
                     MTKPAFRKSLNFANDAATITDSIKLTVDKFEVGQIFPSLVPYYRAKGIGSITLKNISN
                     GTLKNVEIAFKISDKVVGTAKISDIAPNAVVAADVKLDTIPEGKSEYAQLAANITFTT
                     GESYGRKDAYAPLVIHSRNTIDWSNPMTIASFVDPSNKAVRDLATQTIKKGAPKELAT
                     KQLGNAALIFSSLWHTPLKYISDPVSTSFDSNIDTVQFPAETLSRGAGDCDDLTVLLA
                     SLFESVGLATVIITSPGHVFLGVESGSLAGGNVIFNLPQSLFVEVDGALFIPVEATAI
                     GSSFAEAWLKAADIVKKSANSISAFRTREAWKSYPVTSVNSDKVSLNFVEPKDGVVKN
                     VLADIRKAHKDKAPVWAESIHKSKIEHKNPDIKLETLSKEPLAKSVALWLTGKRDEAI
                     TEAANLCSGGTVEGCYNMTVMSMYDALERSDLQTIDANMTKNNYGEAVAMLPPNVINM
                     LSDNGGLGMGDEASKESETKKKIADILKQAREKAGNKGKASNTSKEIKTSHVGGRKAS
                     DATTEASATAEMFFWDKIKK"
     gene            complement(10964..15322)
                     /locus_tag="PL056_04275"
     CDS             complement(10964..15322)
                     /locus_tag="PL056_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin-like protein"
                     /protein_id="HPM46260.1"
                     /translation="MTVDRTTPSFSSNTCDVTVSSGYTNPAGIKAVKAGQTVTANCNI
                     TANETIKGNPVLKVGGYAATRQGSCPGGYNNCYIYTVAGTEGDGNKVITSEVEDLAGN
                     ITASSTGQQVSFDFTPPQLVYYNIFRTPDIEGSTEGQMIHVSAYHPISQQSTVVTFQL
                     FADEHIDAGTVSLVSSPAGFSPLLSSVNNNIAEFNKTVLLANSGIYTFSITWRDKLGN
                     TSGTIPLDWSINANNDPTSGSDIDIDRILFTRVPYGSEDTEGLPRYILTGSAGAVTEA
                     SIASIAAYTLSGSIAGHAAVMPDGSFSMPNMSSGNVAEIFVNPIRKSGASMMPEGVYD
                     PVNDFIQVRKTRYISSMGYKTAGSDLNNPNDWYVTKTDKYSVEYVGTNTSTEADGGLL
                     SRLDANPLESGTASKWILAGRNEHPEASYRTTMCYDSKRGKIVLFGGAYDFYSNETWE
                     WYDGKWTRALVDDPEGDGEPVEAVYGHMAYDNKRGVCVYYGGADKNGNVLDKTWEWNG
                     RSWKRVFPTVSPMGIYGGGFAYDNHRGVMVLAGGVYGAANWQVYEYDGDSWKQMPRVS
                     GVSPSSSGGKMVYDRSRRVMVFAGGGNQDDETLEYAGQKFIQVCGTGTSCTGLTERED
                     VMMAYDAKRKVTVYFGGVQGGTATNTLNEWNGTSWVTKNPAGDKPDAREEGSMVYHEG
                     RQTVILYGGRNGMSTKYNDTWEWNGTKWTRLSNADETWAYPAAKTDYAVGYDESRGVT
                     ILHGGDLNANVLGETWEWNGYGWKKLSTATTVYRAGHTLVYDTNSQKLLMFGGIDNGA
                     PTVHRNEVYEWSGTNWVLRPVLVGTAPSGRVYHSAAYDSVRNKMVILGGRNSSGTMLR
                     QLYEWDNATQAWVYRGVSSPAARINAGMVYDPVKQRTIVFGGRNYSLSPESYGDTWEY
                     NNATNGWNNVIANPWSWGSYYPGPGCPSGESWCWKNNSATESPMPRWGQMMVYDPQMR
                     KTVVSGGMVFQDWSEGIHFTAGDTWIYDSASTSWQELDVINKNAFADRTNIYMHKGFY
                     DSNKNEVASWGGVRYMEDSQLAYYNTNILRTGASELPSHMFTVSLDAAGLTEQMAWRD
                     ISSINLKVYAGATVYSGTSCAEVNGVILYGWADDRWLLLDQSPAPRSSVTELTFSINK
                     PEMISKMLNGESSELYFKIETKGKAGCGNNDEPLLAVDYAEISVDIKEDSIKERYYVS
                     TSSATWAEARADCISRGMDLAVISSQKEADLIESIITTTHYWIGLYEPSVEGEWEWVN
                     GLKAWSGGTDGAGKTWTKWAVDSNNGMYKPNDTANLNCAIFYNYLSRIFWDWTCGSTT
                     RYICEKRDYSYSGASGSKFTNQTACNNMGGDLVSVNSELEEDAIWPHVVQATNYQYIG
                     LTNMSSSDYIYSWNDGDVCWDGNSGTTGTAYGYTNWGTSQPDSTSTDCVAYLYTMDKW
                     YDYSCYYSNWSGICEF"
BASE COUNT         4142 a         3424 c         2880 g         4826 t
ORIGIN      
        1 ctttttgttt ttacaggaat attctggatg acagacccga accattatgc tgatccgctc
       61 tttcacatgc tttcgggcgg agttatgctt ggagcatggt tcatggcgac tgatatggtc
      121 acaagcccga tgtctgttaa aggtcagata ctatttgcag tccttgcagg tgttctttgc
      181 ggctctataa gacttttcgg aggttatccg gaaggttgca gttattcaat acttataatg
      241 aacgcatttg ttccgcttat agataaatac ataaaaccgg taagattcgg aaaggaggtt
      301 aactatggct aaaaagctcg aatcgacttt cccaaacatg gttcttgtac ttacagtgat
      361 ctccatagtt gccgcggccg ctctcggttt cacttacaca aagaccaaaa cccagatagc
      421 tgagatgcag aaacaaaaga aaatttccgc aatagaaaaa gtcgtaccta aattcaacaa
      481 caatcctgat tctgagaaat atacagttaa agagcttgcc gggctggagt tctatcctgc
      541 aaagatggac ggagtccttg taggaacagc ggtccagaca ttcacagaaa agggatatgc
      601 aggcagaata gatatcatgg tcggttttaa cgataaaatg gattttgtgg attatgtaat
      661 actgaacgca aaagagactc ctggtcttgg aaccaagctt tccgacaaca agttcaagag
      721 ccaatttcct ggaaagaatc ctgctacatt caatcttaaa gttaaaaaag atggtggaga
      781 tgtagatgca ataacagctg ctacaataag ttccagggca ttcctggatg ctgttaataa
      841 agccgttgaa ggtcttaaaa agggaggcga ataatgagtc tttttaaaga gttcagcaag
      901 ggaataataa aagagaaccc gacactggta ttgcttattg gaatgtgtcc tcctcttgca
      961 gtaacatcaa gtgccataaa cggaatgggt atgggcattg ctacgacatt tgttctagta
     1021 tgctccaatc ttgtaatagc agctctgaaa aatattattc ctgacaaagt caggctacca
     1081 gcattcattg tcgtaattgc atcttttgtt acgatagtgg atctctcaat gcaggcttat
     1141 acaccggcat tatcggcttc tcttggtgtt tttattccac ttatcgttgt caactgtata
     1201 attcttggaa gagctgaagc atttgctcag aagaatggta ttattccatc aatatttgac
     1261 ggtttgggaa tggggttggg atttacactt gcgctcacta ttctcggagg gataagagaa
     1321 gttcttggca acggttcgat ctttgatatc aagtttgttt ctgaagatgc aacaaccatt
     1381 cttttatttg ttctggcacc gggagctttt ataatgcttg gactgatgat agctgccatg
     1441 aacaaactta tgaagaaagt ataggagggg tgtaatggat ttttatatga aaataatgat
     1501 cgggtctatc ctggtcaaca acataattct tgcacaattt ctcggaatat gtccttttct
     1561 gggtgtttcg aataaagttt ctacttctgt gggaatgagt gcggctgtta tttttgtaat
     1621 gacgctggca acaacggtga catatctttt atattcacgt gtgcttgttc cgcttcatat
     1681 tgagtttatg cagactgtag gatacattct tgtcatagcg gctcttgtgc aaatggtaga
     1741 aataataatg aagaaggttt ctccgccgct atatcaggct ctcggggttt ttcttccgtt
     1801 gataacaacg aactgcgcag ttctaggtgt tgcgatcatc gtaattcaaa agcaattcaa
     1861 tcttgttaca agtgttgttt ttacaattgc aacagcactt ggatttttcc ttgcgctcgt
     1921 tgtttttgca ggaataagag agcagcttga aatgtttgaa ccgccaaaag caatgaaagg
     1981 agctccgctt tctcttatta cagccggcct tctttcactc gcattcatgg gatttgcagg
     2041 gctggtttaa gtcaccgaaa ttcactccgc aaatcatttc attttttctg aacttttctc
     2101 ttccatctct ttccggaaag ttttgtgcaa agaatttatt gctttttgga ttttatatag
     2161 tataatatat atattatact gtatggtaat gatggcaaaa tggttgattt gaaagattta
     2221 gggacaatgg aaaatgagat gatcgctctt gtttccagcg gtgttgttta cacatttgca
     2281 gatattcaga aactgtataa tgagaacaga aataaattcc ctgcttataa cagatctttc
     2341 aaaaagtttc tccgcaaact tcttgaactc ggacttgctg aacggcatat ccttgatgaa
     2401 gagggatatc agaaaaagac tgttttcact ttcggagatt ttgacagtta cgaatgtatc
     2461 agcaaaataa acaaaaatgc ttatttcagc caccacactg cccttttcat gaacagcatg
     2521 actgaacaga taccgaagac ggcttatgtg tcttatgaac tctctcaaac aaaaataact
     2581 gtgacaaggc cggaacttaa gcaggaaaat attgataaag tcttttccgc tccaccccgt
     2641 gaggcacggt attatttatt taaccgcaga caggtcattc tgcatgcttc aaagtttaac
     2701 ggatataccg gagtgattaa aaacagtaac ggcacatttt ttcagacaga tccggaacgg
     2761 agtctgatcg acagtgttgt ccgtccttat ttttcgggcg gaatatttaa tgttttaaat
     2821 gtttttattg aaaacagaga acggtactct atttctaaaa ttgtgaatta tctgaaggca
     2881 ttgaactaca tttatcctta tcatcaggca atcggatttc ttcttgaaaa agcgggattt
     2941 cctgaaaagg atcttgcact ttttgaaaat atgggaataa agcataaatt ctacattgat
     3001 tatgagatat ctgatcgatc attatcaaaa aggtggaatc ttatatatcc ttcagcgttt
     3061 gatgaataga tagcattttc tcaacaacat agtcaaaaat ttcattgtat gacacattct
     3121 tttccctttc aggcagagta tcgagcaatt cacggaagcc gttcctatga tattcacggt
     3181 attctttaat tatcagcagc agatcggcag gaactctttt cttttcaaag atttttttga
     3241 gcattagtgc attctcagcg gatctgatat ttattttcat cttatttata agcatataga
     3301 tatcataaaa atctttcggt ctcggcctgc cgcctgagtt ttcctgactg cggtattctt
     3361 ttgtctgctg acagattgcc cgcattttct caagaatgat catttcagga gaatagacat
     3421 agacggtata tccgtcaaca ttcaccttca ctttgctttc acagtattca tattttgaga
     3481 tatccgcatg gaaagtcttt tttaatcctg cgatgtcaag ggctcttttg cgtttaatat
     3541 cttcgctttc tccaatgaaa gcttctttta cttcaatcaa tttaaaagtt attttatatc
     3601 cgttgcctct tggagtattc cttttttcag gtgccggttt ttcttcaaat ttcaactcga
     3661 ccacttccaa attatcatct ttaaaaaaat cattcagtga tttgaatatt ttcttttcta
     3721 tccttctcag atcgctttca acgaaatcat cttccatgga caggtcaata tcaagtgagg
     3781 gtctttttgt aactttataa ataagttcca gagcgttacc ccctttcagc acgaaaatat
     3841 ccataagttc atcatcggaa aaaaaggaaa gcatgatttt ctttctcata tcaagaagcc
     3901 atgcttcact taattttgca tttggcatat gtttatccgc cgacagttaa taaaccgtca
     3961 cccattttaa cagaaaaaca gagtgagtaa agttagttgt gttaaatctt ttatgacaca
     4021 aaaaaacgct taatttaatc agtttagctt taattgtaaa acatattaaa caacaacaac
     4081 cttgtatttt gaaagcgtga gtttcagaac aaccttgtat tttgaaaatt ttagattttt
     4141 tagatgcctg cggaaatccc acagcttttg aaatcatcaa ctctaagtcg gaaaaactgc
     4201 ctgaaaccaa acacaattct ctgcttttag aattaagaaa atatttttta gtaggcagaa
     4261 tgcccgaaag gaattgtctt ctcaacgagg gaatatgctc agatcccgga acagaaatta
     4321 atgttccttc cactttacta tgccggaagt attttaaatg tttctaatta atgccttctt
     4381 tcactcgcat tcatgggatt cgcagggctg gtttagggaa ttaatcactc tacagttaca
     4441 gttccctgtc ctgacggtgt ctgctgggta agtatgactg cggtcgtagc tccggcggct
     4501 ccaagtacta cgacacttgt tattgtccag aaccaccatg atttatacca ttttgtgggt
     4561 ttttcaagga cattaaaagt tttcacattt tttgctccgg gggcgacata cacatctgtc
     4621 tgaagaggca gataattgtc atttgcagct ctcactttca gataatatct tcccggaata
     4681 agattgccgg cactgtactg cggcttttca aaaggagatt ccgtttcacc gaactggata
     4741 gttccctttt tcacattgaa cgagaaatca acttcaccca gaagtgcttt gtagtcaact
     4801 ccggcgatct gatatgcggc aagctttatc gcattctgca gttcaccttc gtcccctgtg
     4861 taagattcaa gaacacggtt tacaacatca gcatttctga catctatgag ctgaacactg
     4921 attacgaaag tgtcctttac ttttcctaca gctcccgtaa caagctttga aagaccaaga
     4981 gaagcaccta tttcaacgac acattcaaga ctgtcagtgc atgaaactga actgtcaaga
     5041 gccgcttttg aaagcatcgc cttgatatca tcctgagaaa tgacgcttat tcctttgatc
     5101 tggttaagct gtgctgaaag aatggatgtt agagaatcag cagtagtctg ttccattccg
     5161 cgggctgcaa gaggcatgac tgcaagccgg agctcttcac cgtcagcaag tttgaacatc
     5221 tgtttcggag cagaaatacc aagaagaatg tctacagatt ccttgatctt tcctttgaca
     5281 tcgttcagat ctttgccctg aacacttatt ctttcaccta cagttttatt tgcaacattg
     5341 actgtattta tagaaagaat gtattcatca ccaagcttgg taacttttcc ggcaataagt
     5401 gtctcaacat caagtttttt ctggacctca ataagacatt catcgcttcc ttcacagccg
     5461 gtttccgatt ttaactgggc ggtcttgaca acattctgca tttcggcact cgtaacaacc
     5521 gagatacctt ctcttccccc tatatcaaga acgagtgcct cttcaagtga tttggcaaat
     5581 gcctcttcca caccggcggc ctcgactttc tggacaagaa gctttttctg ttcaactgac
     5641 tccttcttct gttcagtgac ttccgttttt ttctgctcaa ccggttcttt ttttgtttcc
     5701 tgtgcagaaa gtacaagcac aaaagaaaaa agaatggtaa ttgccaaaaa tcttttcatt
     5761 tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nttttcagaa tatttttcat
     5881 acaatccctg aagaaccggc atttccttga tgcatggggc acaccaattc gcaaaaaaat
     5941 taagaaccag gattttcttc ttttccttgg aatccttttt gcccacataa tcaccaagcc
     6001 agacaagaga tgaaagcttt cctttctcaa catcaacttt gcgcatgccg aaatcaacca
     6061 ttttgtcacc ttttttcaaa ccttcgcttt tcggttcatc ggcaaaaaga atggaaaaag
     6121 caataacaga aatgatcaaa acaaaaggaa tcctcaagat cttcataaac tactccttat
     6181 tttcattaat tttctctttt tcataattta caggtgatat ctgccacaca gctacttcgc
     6241 cttcatatcc tttaagttcg acacttcttt tataaccctc cggtctcaat cccgcagatt
     6301 caacagtttc ttcagaaaca agaattcctc caatttcaga tttactttca agccgtgcgg
     6361 caaaattcac acctcttcct attgctgagt attcgatgtg accggcaccg ccgaggtttc
     6421 cgacaaacac agtatcagta gcgatgccta ttcttatttt aagctctctt cctgttatct
     6481 ccatccactc tttctgcata tcatgaacct tcttctgcat tgcaacggca cagtcaagtg
     6541 ctttttttgc atgatcaccg aatggtttcg gtgctccgaa aaggatcatc aacccgtcgc
     6601 cgtttatttt gtcaacatat ccattgtgtt cagcagttat cgtcatcatt tcgtcaagat
     6661 aaagccggag agacttcatg atgctctctg cgttgagcga gtttgacaat gatgtataac
     6721 ctgcgatgtc gctgaaaagt attgttatct gccttctttc tgcagagttg ctctgaagca
     6781 ctttaccgcc tgtttcttta agccagctca gtatttcagg agatacatag ttgtcaaatg
     6841 catttgaaag cacttgattg aaaaggaaat atttccatcc gaggtatccg gcacatgcaa
     6901 tatttgcaaa aaagaacggg atgaacatct ccatcggtgg aataaaaata aaactttttc
     6961 tgaaaagaac atagcttgca gaatatatta ctgcaaccca aacagttcct gatatcagaa
     7021 aagccctggc gtgctcaaaa agaactgctg atgccagaag aatgagaaaa ggaatgagaa
     7081 ttattatcca aagagatgtt cttgtgatcc ggatgtcgtt taaaagcgct tctgctctgt
     7141 taagatgaag aaagacaagt ggctcaacaa gtcctgaagg agttggacct atatctttca
     7201 atgtttcatc agttgcacct acaaagacat attttccgtt caccttttct cttaggattt
     7261 tcattttctc atcacttcct gaagactcta tgacatccac catcggaatg accgtgaatt
     7321 cagaagatgt tctgaaatac ggaatgatct cttctttgca gcacccttca taaggtcctt
     7381 tctgcaccat tgcgagggca agatcgggaa cacatccgtc aaagaccttt ttgcagccga
     7441 tgatggttct gttcactctg tcgacatctg aaggaatgac atgaacatga gcaacaagag
     7501 gattgaattt caaaacaccg ggaaccattt tttcaaggaa tttgaatttt gactcttcga
     7561 tctcttcttc agaaagatcg ctgcatggac agtttattcc gctgttttca ggagtcggcg
     7621 gatattcaac gacctttgaa gggtcttttc tgtaatctgt gtttacaaag cttccgagaa
     7681 ttgccccggc tttggagctt tcttctccga gaacctcttc agattcgacc atccgtttta
     7741 atgcttctgt atccgtttct tcctttctgt cagtgaaaaa aagatcaaaa ccgatttttt
     7801 ttgcacctgc ttcggtaaga acagatatca ttgctccgta aagtcccctg tcgaccggcc
     7861 aaccgaaaac agaaagcgaa tactggtcaa cagcgatcag aacgctctcc gccggtgctt
     7921 ctggctcaac tgatatcatc atcaggtcaa gaaatgaaaa atcgagtttt ttaaccgcag
     7981 gagtatgaac gactgctgtt ccgacaagaa atgccgcaac aactacaaga agttttaaat
     8041 acggagaact tttcatgaat tatttcttta tcttatccca gaaaaacatt tcagcggtag
     8101 cagaagcttc tgttgttgcg tcgcttgctt ttcttcctcc gacatgggat gtttttatct
     8161 cttttgaggt gtttgacgcc tttcctttgt tcccggcttt ttcccttgcc tgcttaagga
     8221 tgtcagcgat ctttttcttt gtctcggatt ctttggatgc ttcatctccc attccaagtc
     8281 cgccgttatc tgaaagcatg tttattacat tcggaggaag catggcaacg gcttctccgt
     8341 aattattctt ggtcatgttg gcatcaattg tctgaagatc acttctttca agagcatcat
     8401 acattgacat tacagtcatg ttgtaacacc cttcaacagt acctccgcta cacagatttg
     8461 ccgcttcggt tattgcttca tctcttttac cggtaagcca gagagcaaca ctttttgcaa
     8521 gtggctcttt tgacagcgtt tcgagcttaa tatccggatt tttatgctcg atcttgcttt
     8581 tgtgaatact ttcagcccag acaggagctt tgtccttgtg tgcttttctt atgtcggcca
     8641 gaacattttt tacaacgcca tctttcggct caacaaagtt caaactgacc ttgtctgagt
     8701 tgacagatgt tacaggataa cttttccacg cctctcttgt tctgaaagca ctgattgaat
     8761 ttgcagattt cttaacaata tcagctgctt tgagccatgc ctctgcaaat gatgagccaa
     8821 tcgcagtcgc ttcaaccgga ataaaaagag caccgtcaac ttctacaaaa agagattgag
     8881 gaagattgaa aattacattt ccgcctgcga gtgagccact ttcaacaccg aggaacacat
     8941 gtccgggaga agttatgatg actgttgcaa gaccaacaga ttcaaaaaga gaggcaagaa
     9001 gaactgtaag gtcatcacag tcacctgcac cccttgaaag agtctcggca gggaactgga
     9061 ctgtatcaat atttgaatca aaactggtac ttaccgggtc gctgatatat tttaagggtg
     9121 tgtgccagag tgatgagaaa ataagagccg catttccgag ctgttttgtg gcaagctctt
     9181 taggagcacc tttcttgatc gtctgtgttg caaggtctct taccgctttg tttgacggat
     9241 cgacaaaaga agcaatggtc atcgggtttg accagtcgat agtgtttctt gaatgaatta
     9301 ccagaggagc gtatgcatct tttctgccgt aactttcacc agttgtaaaa gtaatatttg
     9361 cggcaagctg agcatattca gatttccctt caggtatcgt atcaagtttt acatctgcgg
     9421 caacaaccgc attcggagcg atatcgctga tctttgctgt tccgacaact ttgtcgctga
     9481 tcttgaaagc gatctcgaca tttttaagag ttccgtttga gatattcttc aatgttattg
     9541 aaccgatacc ctttgctctg taatatggaa caagtgaagg gaatatctgc ccgacttcga
     9601 atttatctac tgtgagcttg atggaatctg ttatcgttgc cgcatcattt gcaaaattaa
     9661 gtgattttct gaaagcaggt ttggtcatgg atatgcggta tgtggcatcc attgttctta
     9721 ccgctttgtc catcgcttca aaaggatctc cggcagttcc tttcgcaact gcgatctttt
     9781 tgccgtcttt tgaagttatg agatctatga cagaaagcca ttcatcgtct ttttcgacaa
     9841 cttctgcaaa aacgacaagg ggcacatcat tcttttttgc ttcagataca gcggaatcct
     9901 ttgaaccttt aagcgtttcc gattccttga tctcaaaata ttcaagattc atcaaataac
     9961 ttgtaagaag gtccggacct tttgaagcaa tgagatcagc catcgttttc ttgccaccgc
    10021 ttttcggagg agtgatgtaa gccgcaacac ctcttgaata tgcctgaccg aaatcaaatt
    10081 ctgcgtgaat gacccttcct gcctcataat tcacacgaag actcaacttg tctgaagtgt
    10141 caatggcttc tctgagcttg ctgtaatttg cagcaccttc tccggaaacc gaaatttttg
    10201 aaagatcttt tttaaacatt acgacagcag catcttttac gccgtcaacc tttttaagtt
    10261 ctccgtttat ctgatcgatc tttgttttct gggctacacc ggtgatgatg atgtccatgt
    10321 tccattttcc ttcatttgac cagttctttg accactgatc aacagccgca tcagcgtttt
    10381 tcctcatatt cttttttaca acagtattga tcgcctgaag tgcgttaagt cctgttgctg
    10441 tgtaattctt tgaatcagca aagatgacat ctcctgaatc aactctgatc acttttgaat
    10501 tcaaaacaca gaaccagctc tttatgttgc ttcccatgat tgaatctgag tttttatcac
    10561 aggcaagctg ggctgaaacc agcgcatcag cattaagtac cgaaagttcg gtcggaacct
    10621 ttccttccgc aacgagatct gaaagagccg ctttctgggc tttaagtgca gttgaaagtt
    10681 caactattct catgttctta ccggtgagaa tgcttgccat ctctgtttct atggatgcgg
    10741 aatcatactc ttttccgtca actgtcattt tcggcagaag aactattctc aagtcaacgg
    10801 cctgtattga aaaataacag aacatcatta aaactacagt taccatgcta aaaaaatttc
    10861 tcatttttcc ctccatgaaa taccattaaa aagcattagt gcataatctt atattttaga
    10921 ggtataaata gcaagttttt gaaatggtct aattcagttt ctatcagaat tcacagattc
    10981 cgctccagtt tgagtagtag caggaatagt cataccattt atccatagtg tataagtatg
    11041 ccacacagtc agtacttgta ctatcaggct ggcttgtgcc ccagttggtg taaccatatg
    11101 ccgttcctgt tgtaccgctg ttcccgtccc aacacacatc accgtcattc catgaataaa
    11161 tataatcact tgatgacata ttggtcagtc cgatatactg ataattagtt gcctgaacaa
    11221 catgcggcca tattgcatct tcctcaagct cgctgttaac gctgacaaga tcaccaccca
    11281 tattattgca tgctgtctgg ttcgtaaatt tactgcctga tgctcctgaa tatgagtaat
    11341 ctcttttttc gcatatatag cgggtagtgc ttccgcatgt ccagtcccag aatatccggc
    11401 tcagataatt gtaaaaaatc gcacaattta aattagctgt gtcatttggt ttatacatcc
    11461 cattgttgct gtcaaccgcc cattttgtcc aggttttacc tgcgccgtct gtacctccgc
    11521 tccatgcttt caatccgttt acccattccc attcaccttc aacggaaggc tcataaagac
    11581 ctatccagta atgagtcgtt gttataatgc tttcaatcag atcggcttct ttctgtgatg
    11641 atattactgc aaggtccatc cctcttgaaa tgcaatcagc acgggcttct gcccaagttg
    11701 cagaacttgt ggagacataa tatctctctt ttatggagtc ttcttttatg tcaacggata
    11761 tttcagcata gtcaactgca agaagcggct catcgttgtt tccgcaacct gctttccctt
    11821 tcgtttcgat cttgaaataa agctctgaag attccccgtt taacatcttg cttatcattt
    11881 ccggtttatt tattgaaaat gtcagctctg tcacagagct tctcggtgcc ggactctggt
    11941 caagtaacag ccagcggtca tcagcccatc cgtaaagaat aacaccgttc acttcggcac
    12001 aactggttcc gctgtaaaca gttgctccag cataaacctt cagatttatt gatgaaatat
    12061 ctctccacgc catttgttcc gtaagtcctg cggcatcaag cgatactgta aacatatggg
    12121 aagggagttc tgacgctccg gtccttaaga tattggtgtt gtaatacgca agttgagaat
    12181 cctccatgta tctgactccg ccccagcttg ccacttcatt cttgtttgaa tcataaaaac
    12241 ctttgtgcat atatatgttt gttctgtcag caaaagcatt tttatttatg acatcaagct
    12301 cctgccagga ggtagaagca ctgtcatata tccatgtatc accagcggta aagtgaatac
    12361 cctcgctcca atcctggaaa accataccgc ctgacactac cgtctttctc atctgaggat
    12421 cataaaccat catctggccc catctcggca ttggactttc agttgcgctg ttattcttcc
    12481 agcaccatga ttcacctgat ggacatcctg ggccgggata ataacttccc cagctccatg
    12541 gatttgcaat aacattattc catccatttg tcgcattgtt gtattcccat gtatcaccat
    12601 aactttcagg gcttaagctg taatttcttc ctccgaaaac aatagttctc tgcttaactg
    12661 ggtcataaac cattccggca tttattcttg cagcaggact cgaaacacct ctatataccc
    12721 acgcttgtgt agcattatcc cattcataaa gctgtctgag cattgttcct gaactatttc
    12781 tgcctcctaa tattaccatt ttattccgta cactgtcata agcggcacta tgataaactc
    12841 tgcctgaagg tgccgtacca accaaaacag ggcggagcac ccagttcgtt ccgctccatt
    12901 catacacttc atttctgtga actgtgggag caccgttatc aataccgccg aacataagga
    12961 gtttctgaga atttgtatcg taaaccagag tatgtccagc tctgtaaact gttgtcgcag
    13021 tagagagttt tttccagccg taaccgttcc actcccaggt ttcacccaga acattggcat
    13081 ttaagtctcc tccatgaaga atagtaactc cgcgtgactc atcataacct accgcataat
    13141 cagtttttgc tgctggatat gcccaggttt catctgcatt ggaaagcctt gtccactttg
    13201 ttccgttcca ttcccatgta tcattgtact ttgttgacat tccgtttctt ccaccgtaaa
    13261 ggataacagt ttgacgccct tcgtgataaa ccattgatcc ttcttctctt gcatccggct
    13321 tgtcacctgc cggatttttt gtcacccacg atgttccatt ccattcatta agcgtatttg
    13381 ttgcagttcc accttgaaca cctccgaaat agactgtgac ttttcttttt gcatcataag
    13441 ccatcattac atcttcgcgt tccgttagac ctgtacagct tgttcctgtt ccgcaaacct
    13501 gaataaattt ctgtccggca tattcaagag tttcatcatc ctgatttccg ccgccggcaa
    13561 aaaccataac ccttctgctt ctgtcgtaaa ccattttgcc gcctgagctt gaaggggaaa
    13621 cgccgcttac tctcggcatc tgtttccaac tgtcaccatc atattcataa acctgccagt
    13681 ttgccgctcc atatacacca cccgcaagaa ccatcactcc gcgatgattg tcataggcaa
    13741 atccgcctcc atatattccc attggactca cagtcggaaa cactcttttc cagctccttc
    13801 cattccactc ccaagtctta tcaagaacat ttccgttttt atccgctcct ccataataga
    13861 cacacacacc tcttttgttg tcataagcca tgtgtccgta aaccgcttca actggctcgc
    13921 cgtccccttc cgggtcatca accagagcgc gagtccattt tccgtcatac cattcccatg
    13981 tctcattact gtaaaaatca taagctccgc caaaaagaac tatttttccc ctttttgaat
    14041 cataacacat tgttgttctg tatgatgctt caggatgttc gttcctgccg gcaagaatcc
    14101 atttagaagc ggtcccgctt tcaagcggat ttgcatcaag ccggcttaac aagccaccgt
    14161 cagcttccgt tgaagtgttt gttcccacat attcaacaga atacttgtca gtttttgtca
    14221 cataccagtc atttggattg ttaagatcac tgcctgctgt tttgtatccc attgaagaga
    14281 tatatcttgt tttcctaacc tgtataaaat cattaaccgg atcataaacc ccttccggca
    14341 tcattgaagc accgcttttc ctgatgggat ttacaaatat ctcggccaca tttccacttg
    14401 acatattcgg cattgagaaa cttccgtcag gcataacagc tgcgtgtcct gcaatgcttc
    14461 ccgaaagagt ataggcagct attgaagcaa ttgatgcttc agtaacggca ccagcagaac
    14521 cggtaagtat atatcttggc agtccttctg tatcttcaga accgtatgga actcttgtaa
    14581 aaagaattct gtcgatatct atatcgcttc ctgaagttgg atcattgttg gcgttaatgc
    14641 tccagtcaag aggtattgtt ccgctagtat ttccaagctt gtcacgccag gtaatgctga
    14701 aagtataaat gcctgaatta gccagtagaa ctgttttatt aaattcggct atgttattat
    14761 taacagaact tagcaaagga ctgaatcctg ccggagatga aacaagcgaa actgttccgg
    14821 catcaatgtg ctcatccgca aaaagctgaa aagttacaac tgtactctgc tgtgagattg
    14881 gatggtatgc actgacatgt atcatctgtc cttctgtaga accttcaata tcaggtgttc
    14941 tgaaaatatt gtaataaaca agctgaggcg gagtaaagtc gaaagaaacc tgctgacctg
    15001 ttgaagaagc agtaatgttg cctgcaagat cctcgacctc tgatgttatc accttatttc
    15061 cgtcaccttc agttccggca acagtataaa tatagcagtt attgtatcca ccgggacagg
    15121 agccctgtct tgttgcggca tatccgccga ctttcaaaac aggattgcct ttgatggttt
    15181 catttgcggt aatattacag ttagcagtaa ctgtctgacc tgctttaaca gctttgattc
    15241 cagcgggatt tgtgtatccg cttgaaacag tcacatcaca ggtgtttgaa gaaaaagaag
    15301 gtgtcgttct gtcaactgtc acagatctcg acctgttttc cgttagcacc gaaataaggt
    15361 cctacattga ca
//
LOCUS       DAOOYB010000046        15174 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_3915_length_15174_cov_12.113764, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000046 DAOOYB010000000
VERSION     DAOOYB010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 15174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15174
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3915_length_15174_cov_12.113764"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="PL056_04280"
     CDS             complement(<1..382)
                     /locus_tag="PL056_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002628183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HPM46261.1"
                     /translation="MKSRSIISKHYTEIVKDQKEEVKSNELFKDLIEIERYKKPKTRA
                     ECLRMRRPCLFITCKYHLFLDVNPSTMSIKFNFPEKEIWDLNHTCALDIADKGGATLE
                     KIGVAMNLTRERVRQIEMRAINKLK"
     gene            complement(401..847)
                     /gene="nusB"
                     /locus_tag="PL056_04285"
     CDS             complement(401..847)
                     /gene="nusB"
                     /locus_tag="PL056_04285"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HPM46262.1"
                     /translation="MGVRRNSREKAVQIMYMLHFNKILSLSEARDAYYEHLNIVNTNR
                     RNEDEFEFVTYLLDQVKMNQFYIESLVEAALKNWKIERVGIIEKNILRISVAEFLDSN
                     NKVPYKVTINEAIEIARKFCSRDSGSFVNGVINRVADILNIKDYKI"
     gene            complement(903..1181)
                     /locus_tag="PL056_04290"
     CDS             complement(903..1181)
                     /locus_tag="PL056_04290"
                     /inference="COORDINATES: protein motif:HMM:NF038144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PxxKW family cysteine-rich protein"
                     /protein_id="HPM46263.1"
                     /translation="MINESEKTKILIKLAGRDKHYVDRRSPVVELCDGCARTEEISGT
                     KFCLKYQVPSFHWENEQICAFATHIERKRADKKKKINPIKLSKRLRKR"
     gene            complement(1222..2565)
                     /gene="gdhA"
                     /locus_tag="PL056_04295"
     CDS             complement(1222..2565)
                     /gene="gdhA"
                     /locus_tag="PL056_04295"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013499074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HPM46264.1"
                     /translation="MNLYVNSVFEDIKSRYYWEKDFIQAVKEVFESIGPLMSANLKYH
                     SSRVLERIVEPERIICFRVPWIDDKGKLNINRGFRVQFNSAIGPYKGGIRFHPDVTLD
                     TLKFLGFEQTFKNSLTGLPMGGGKGGSDFDPNGKSDNEIMTFCQSFMIELFRHIGPNT
                     DVPAGDIGVGGREIGYLFGMYKRLANEFSGVLTGKGLSFGGSLIRPEATGFGGIYFAQ
                     EMLDKMNDSIEGKKIAVSGFGNVAWGVVRKATELGAKVITLSGPDGYVYDKDGVSSEE
                     KCNYLITMRSSGRNRVQDYAEKFGAAFYPDKKPWGIPTDIAIPCAIQNELNLQDAKKL
                     VSNGCRYIIELSNMGCTSEASSYASKKITFVPGKAANAGGVAVSGLEMSQNSIRFNWS
                     AEEVDWKLKEIMRRIHDTCADTAVAYGLKANNYISGANIAGFKKVADAMISQGAV"
     gene            complement(2913..4298)
                     /gene="gltA"
                     /locus_tag="PL056_04300"
     CDS             complement(2913..4298)
                     /gene="gltA"
                     /locus_tag="PL056_04300"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HPM46265.1"
                     /translation="MKKTDIPAQIMPLCDPEKRKKNMDEVPLGYTMEMAVTEASRCLD
                     CKNAPCVKGCPVGIDIPKMLNLTAEKKFTEALETVREMNFLPAICGRVCPQENQCQKE
                     CTLGKMKGGTVPPLSIGNIERFLADNYSDEIIIEKTASPTGKKIAIAGSGPGGLTCAG
                     ELAAMGHKAVIFEALHKPGGVLIYGIPEFRLPRYILSREIEFLRRLGVELVMNTIVGK
                     TITVDEILKEYDALYVTTGAGVPHFPDIPGTHLYGTSSANEFLTRVNLMESWKKDSRT
                     PLFVGETTIVIGGGNVAMDAARTALRTGSKRVIVAYRREEHDMPARREEVIHAKEEGI
                     EFMFLISPEKIVENEHGHTGGVLFRKMRSEINPEGGKNRIFDTDETFLIDCDAVIFAT
                     GTSPNPIVAKSLPSLATNKAGGIAVDMNNMKTSVPGVFAGGDAVSGAATVILTMLHGK
                     TAAKAINEFLK"
     gene            complement(4310..5140)
                     /locus_tag="PL056_04305"
     CDS             complement(4310..5140)
                     /locus_tag="PL056_04305"
                     /inference="COORDINATES: protein motif:HMM:NF004862.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HPM46266.1"
                     /translation="MEKIISNTVIAKDIHKIVITAPRITRSWRPGQFIIVRFSEQSER
                     IPLTVCKNDEKNGLITLIVQSIGEGTKEICSGEEGSFINDVAGPLGKPAEIDENLKNV
                     LFISGGIGAAPLLPVMQYLHDRDISCVTLAGTKSRNTLVLEEEMRELSYNRLHISTDD
                     GSIGRKGFVIALIEEAVKKYVPFDRAFVAGPVKMMAATVKELQKFNIPSTVSLNPIMI
                     DGTGMCGGCRVTVNGETKFACIDGPEFEGDLVDFDSLIKRLSSYGHQHNHNCRIASSQ
                     "
     gene            complement(5168..6364)
                     /locus_tag="PL056_04310"
     CDS             complement(5168..6364)
                     /locus_tag="PL056_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014959333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HPM46267.1"
                     /translation="MNFKNAIGINGLGRIGKLTLWNLILSDDFDKIVINIGREAGKSL
                     SDLIDYVKNDSTYGSLESFLFGYKGKENIEITDDENGETVFFGKKVKFLRKERSPEKI
                     SWDREGVSIVIDTTGKFTDPSEDPSSSGNLRGHIISGAKKVVLSAPFKFKGKNKVMPD
                     DSVMLVYGINHQSFNPSKHHVISAASCTTTALAHMIKPLLDHEETSSILTASMSTIHS
                     MTNSQSVLDSVPSSGTSDLRKNRAAGNNIILSTTGAAKALEYILPEMKNIGFMADSVR
                     VPVMTVSLINLNLTFNSPLDSNGFPSISREVINRIYKDAAEGKYIDSIHFSQRQNVSG
                     DLKGKMSAVVIESNDTHTRTGFIDIPPLSNIPVTHAKIFGWYDNEMGSYVNSLCRLVS
                     YISKSI"
     gene            complement(6390..10478)
                     /locus_tag="PL056_04315"
     CDS             complement(6390..10478)
                     /locus_tag="PL056_04315"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HPM46268.1"
                     /translation="MANIVHKSEALRANMLETHSVHNNFSEKELFLLEIVAPFPMVTK
                     RLTDYLKEKHHRFPDLDEIVEGYRWIILENLWSFMKHPEREKIFICFADELVSITLFK
                     LNQSQLHRVINTLLGAVEILYKFSGQETTATSSLAYYVDILDKMLSTDNLGLIESSSY
                     LKKYLAGVATQAALQNTVFNIMKKSLIKNILYWKTIQPFAVWEYGLGIEYGPESKEVD
                     EMLKTQFPSKLFEDSAEMIHNAKSFEDLLVVPDFSFFTENLRRSLDLISTPESRIYYI
                     FYLLGLESMAGMREHLLFDLNRILKTVFLDDNEKSKLFVTRIFNFFKPVQNRFQSAVL
                     DCIKTLGIEIVSKAGPELIDHYFEKVIEAGFVYPEVKGVSSDWQIIMNRNHIKNIRTW
                     LEIIAVDPSKCTKLLSALLINLKVGGVFISDTDLFQNDVTKFLNSRISSSYHIARQIA
                     VLFPVYFNEIGAEGELRKISTAIDELFHRNDRLIHFLRKQIHAESNNTHIELVKIIFD
                     FWVSTKKEILAGRVPLDVFESLDISEHSFLKIHEIASSVLEKVSGDVDELFSMDTGEL
                     FKLLDSMKIPESNELKKVEYLFKLNFLLREKYYFNPGDVSKKLRNFNYIPKELIESLE
                     NELSAGNWKKALNCSFSIMSYLKNTVLSAEKTEGVENIYYKRHIAIGIPSMYGEYREP
                     KFEALGMIFRMEIFVSSLMDRMISNAKLNYISAETLSIVTEIIDLFREGLELGGITNE
                     NLISDLNILKYSMESSSFSINQYTNIFEFLAEDISKIVVDFFLGIHDRNLKLILEQHI
                     EKSDLKFSDEKEKECYIHKRSEEFYRDVISSSFLIQKVDNLINNVLSLFREMPADISN
                     ENIHLIMNYRSDSVISKLCEQSDELDNQVFLGAKGYFLKKIRSYGMPVPPGFILTTEL
                     FRRRKALIKHPEIKKEIRKMIMENLKEIERSAGLKYGDPSNPLLLSVRSGSAISMPGA
                     MNTFLNVGMNDEFAEKLSKKDNYGWTSWDCYRRLLQSWGMSFGTNRDVYDRIMSDFKK
                     IYKVEKKIEFKPAVMKKIAKEYMKILEVSDIFFPQDLEEQLICSIHSVFDSWDSERAE
                     VYRKKMNIAPEWGTAVIIQKMVLGNINYDSGTGVIFTREPFSGERDLKLYGDFVMCSQ
                     GEDVVGGLVHPYPVTEKQRFSMKDAPEISMEKDFPEIYSELGKISSQLVSDKGFGHQE
                     IEFTFETKNSGDLHILQIRQYHQNEPWLSVSIPENATQIGEGTGIGGNTLMGRAAFSQ
                     EDLMKIKKKYPEEKIIVIRPDTVSDDIGIIFEADGLLTSRGGATSHAAVTAARLGKTG
                     VVNCRALVLNEEKKLCHINSITIKAGEKIMIDGKSGLIYGFL"
     gene            complement(10650..12020)
                     /locus_tag="PL056_04320"
     CDS             complement(10650..12020)
                     /locus_tag="PL056_04320"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPM46269.1"
                     /translation="MKSVFPSEFLLLRNIKLLYVEDDDDLRNTVFVFLKDFFHTIHLA
                     SNGSDGLKKFIQHRPDIVITDLRMPYKDGIQLTKEIKAINRNIPVILNTAFAEIPDLI
                     SAIEAGVDRYLQKPVDEKNILNVLYDLSLPLMQQEQIRSLQEQNSQENWFFAKSPKMK
                     EVFDKARSVAWTPFSVILRGETGSGKSYLARAIHDMSGRKNHPFVKLDLGTLPENLIE
                     SELFGHEKGAFTGADKKRYGAFESANNGTIFLDELENASAHLQSRLLRVVEEKTIVPV
                     GSNTPTNIDVRIISASNKPLFELVKEGKFREDLYYRLAEFEIILPPLRERKEDVTWLA
                     SRFMIEAADELKKQVYNISKEAQEYLCDRPWYGNVRELRNFIRKAVLLSKGSAITLDD
                     ISGINQESLKHTGENIQTRNFMSLADNEREYIEKALLITDGNKTKAAMLLGIDITTLR
                     RKLKKY"
     gene            12271..12684
                     /locus_tag="PL056_04325"
     CDS             12271..12684
                     /locus_tag="PL056_04325"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPM46270.1"
                     /translation="MMKKKVLLVDDDADVVAQNKMFLESAGYFVESAGSAGEAEKKLD
                     SFDPDIVVVDIMMEHPKAGVELARKLTDPDNDKRYPVIFLTSYGANPELLDEPDYIWS
                     KTVNVLSKPADPLKLLDEINGYFEFLMLQSDQVKG"
     gene            12762..13133
                     /locus_tag="PL056_04330"
     CDS             12762..13133
                     /locus_tag="PL056_04330"
                     /inference="COORDINATES: protein motif:HMM:NF039992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SxtJ family membrane protein"
                     /protein_id="HPM46271.1"
                     /translation="MISKKMALDSGLALVLISLIVFFITKNIYAIYFATGFTVVCMTV
                     PIVFKPFAKLWFGLSHFIGTFLSKIILSIVFYTVITPVALLRQVMGKDSLMLKKFKKG
                     TDSVFVERNHTYKSEDILKPY"
     gene            13143..13292
                     /locus_tag="PL056_04335"
     CDS             13143..13292
                     /locus_tag="PL056_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009106868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HPM46272.1"
                     /translation="MEFIKDLWGFLRVRKKYWMIPAIFVLLLFGVLIVLSSGSAIAPF
                     IYTIF"
     gene            13308..15167
                     /locus_tag="PL056_04340"
     CDS             13308..15167
                     /locus_tag="PL056_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015863045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase"
                     /protein_id="HPM46273.1"
                     /translation="MRQIILGISAFYHDSAASLLIDGEIIAAAHEERFTRKKHTAEFP
                     VNAVKYVLDEAGIALDDVTVIAFYDKPYIKFERLLETYHAFAPHGLKSFLSAVPVWIK
                     EKLFMKDMLKSEFEKLGTKIPQIVFPEHHLSHAASAFYPSPFADAAILTLDGVGEWAT
                     TTICHGTGKDIKILKELHFPHSIGLLYSAFTHYCGFKVNSGEYKLMGLAPYGNPDSEE
                     REIFKKKTLENLVDVREDGSILLNQKYFDYATGLSMTNDDKWQELFGIPRRKSESDLN
                     QSYMNMSLAIQEITEEVVIKLAKTAKELTKSDNIVLAGGVALNCVANGKLLREKIFKG
                     LWIQPAAGDAGGSLGAALAAYYIHLNNERTKIDKRDSMKGAYLGPEFGDVEIEKVARK
                     YQAKFVKYDDFDKLCDKTADLLNGDIVIGWFQGRMEFGPRALGNRSILGDSRSPEMQK
                     KLNLKIKYREGFRPFAPSVMEEEIRTFFDLDDITPYMLLVAPVQEKRRNKVPANYNSL
                     PLYERLYHLRSDVPSITHVDFSARIQSVSKETNLRYWQLIDTFRQKTGFGVIVNTSFN
                     VRGEPIVCTPDDAFRCFMRTEMDYLVMGNYFFDKKEQPGWDKKDNWKEEFKLD"
BASE COUNT         4289 a         3172 c         2788 g         4925 t
ORIGIN      
        1 ttttaagttt atttattgcc ctcatttcta tctgccgtac cctttccctc gttaaattca
       61 ttgcaacgcc tattttttca agagttgctc ctcctttatc ggcaatatcg agcgcacagg
      121 tgtgattaag atcccaaatt tctttttcag gaaaattaaa ttttatggac atagtggaag
      181 gatttacatc aagaaaaaga tgatacttgc aggtaatgaa cagacaaggt cttctcattc
      241 taaggcattc tgctctggtc tttggctttt tatatctctc tatctctatc aagtctttga
      301 aaagttcgtt acttttcact tcttctttct ggtctttgac tatttcagtg tagtgtttgc
      361 tgataattga cctgcttttc atttaaatcc ctcatgaata tcaaatctta taatctttta
      421 tatttaaaat atcagctact ctgttaataa ctccattaac aaaggaacct gaatccctgc
      481 tacaaaactt tcgtgcaatt tcaattgctt catttattgt aactttatat ggcaccttgt
      541 tgttgctatc caaaaactct gcaactgaaa ttcttaaaat atttttctca ataataccta
      601 ctcgttcaat tttccaattt ttaagagctg cttcaactaa tgattcaata taaaattgat
      661 tcattttcac ttgatccaaa agatatgtta caaattcaaa ttcatcttcg tttctgcggt
      721 tagtgtttac tatattcagg tgttcatagt aagcatccct agcttcagac aatgaaagaa
      781 tcttgttaaa atgtaacatg tacataattt gcacagcttt ttctcttgaa tttcttctaa
      841 ccccaatttt tttatcctct tacaaattat tgaacagaat cttttaattc agtgttccgc
      901 ttctacctct tccttaagcg cttagaaagt tttatcggat tgatcttttt ctttttatca
      961 gcccgtttcc tttcaatatg tgttgcaaat gcacatatct gttcattttc ccaatgaaaa
     1021 gacggcacct gatatttaag acagaacttt gttccgctaa tttcttctgt tctggcacaa
     1081 ccatcacaaa gctcaacaac aggacttctt ctatcaacat aatgtttatc tctgccagcc
     1141 aattttatta atattttcgt tttttcagat tcatttatca tttcaatctc cctttttctg
     1201 aattacttaa attaattaca attataccgc tccctgagat atcatcgcat cagcaacttt
     1261 tttaaatccg gcaatatttg caccagatat ataattattg gctttaagac catatgccac
     1321 ggccgtatca gcacatgtat catgaatacg ccgcattatt tcttttaatt tccagtcaac
     1381 ttcttcagct gaccaattga aacggatact attttgactc atttcaagac ctgaaacagc
     1441 aactccgcct gcatttgcag ccttcccggg aacaaatgtt atttttttag aagcatagct
     1501 tgaagcttct gatgtacaac ccatgtttga aagttcaata atatatctgc aaccatttga
     1561 gacaagtttc tttgcatcct gaagatttag ttcgttctgt atagcacaag gtatggcaat
     1621 atcagttgga attccccaag gttttttatc aggataaaat gcagctccga acttttccgc
     1681 ataatcctgt actctattcc ttccacttga cctcattgtt ataagataat tgcatttctc
     1741 ttctgaagaa acgccgtctt tgtcatatac ataaccgtca ggtccagaaa gggttatcac
     1801 ttttgcaccc agctcggttg cttttctaac cacaccccat gcgacatttc caaaacccga
     1861 tactgcaatc ttttttccct caattgaatc attcatttta tctaacattt cctgagcaaa
     1921 ataaatcccc ccgaatccag ttgcttccgg acggatcaat gaaccgccga aagacagacc
     1981 cttcccggtc agcacacctg aaaactcatt tgcaagtctc ttatacatcc caaacaaata
     2041 acctatctct ctccctccta caccaatatc tccagcgggg acatctgtat ttggaccgat
     2101 atgacgaaaa agttcaatca taaaactctg gcaaaaagtc ataatttcgt tatcgctctt
     2161 tccgtttgga tcaaaatcgg aaccaccttt tccacctccc atgggaagac cagtcaagct
     2221 gtttttaaaa gtctgttcaa aacccaaaaa ttttaatgta tcaagggtta catcaggatg
     2281 aaatcttata ccgcctttat atggtcctat agcactatta aactgaacac ggaaccctct
     2341 gtttatatta agcttccctt tatcatcaat ccatgggacc ctaaaacaga taatcctttc
     2401 cggttcaaca attctttcaa gaactcttga tgagtgatat ttaagatttg cagacatcaa
     2461 tggaccaatt gattcaaaaa cttccttgac agcttgaata aaatcttttt cccaataata
     2521 acgggattta atatcttcaa aaactgaatt tacataaaga ttcatttcta tctcctaata
     2581 aaaaaaataa ataattattg aatcctgctg atgattctac ttgtgcttta gctcaaagat
     2641 gaaaatctct tttgaatcct gtttttttac aaagatggta acaattccac attttatcag
     2701 gcacgtgctt ttttttcttt taaaatcatc ctcctttaaa aacatcttga ggttcagccc
     2761 tcaacaatga tttagcaaaa ggcatgccac aaattagatt gaaaaacact aaattttaca
     2821 gcaagttaac ttagacagaa aaaagtctta atttgtcagg gcgatttgct ctggcaaatt
     2881 gccctgacaa caacttgtgt tgttgatcct tattacttta aaaattcatt tattgctttt
     2941 gcagcagttt tgccgtgaag cattgtcaat attacagtcg cggctcctga tacggcatca
     3001 cctcctgcaa atactccggg aactgaagtt ttcatgttgt tcatatcaac tgcaatgccc
     3061 cctgctttat tggtagcgag ggaagggagg cttttggcaa caatggggtt gggacttgtt
     3121 ccggttgcaa atataactgc atcacagtcg atcagaaaag tttcgtctgt atcgaaaatc
     3181 ctgtttttgc ccccttccgg atttatttcc gatctcattt ttctaaaaag gactccacct
     3241 gtatgtccat gttcattttc aacgattttt tccggtgaaa taagaaacat gaattcaatt
     3301 ccttcctctt ttgcatgaat gacctcttct cttcttgcag gcatatcatg ctcttccctt
     3361 ctgtatgcaa cgatcactct tttgcttccg gttcttaatg cggttctggc ggcatccatc
     3421 gccacattgc ctccgccaat tacaatagtg gtttccccga caaaaagagg tgttcttgag
     3481 tcttttttcc atgattccat aagatttacc cttgtcagaa attcatttgc agatgaagtt
     3541 ccgtatagat gggttcccgg aatgtcagga aaatgcggaa ctcccgcacc ggtcgtaaca
     3601 taaagtgcat cgtattcctt caggatctcg tcaactgtga tggtcttgcc gacaattgta
     3661 ttcataacca gttccactcc cagtctgcgc agaaattcga tctcacggct taaaatgtat
     3721 ctgggaagtc taaattcggg aataccgtaa ataagaactc ctcccggttt atgaagtgct
     3781 tcaaagataa ccgctttgtg tcccatcgcc gcaagttcac cggcacatgt aagacctccc
     3841 ggtccgctgc ctgcgatcgc gatctttttt cctgtaggag aggcggtttt ttcaatgatg
     3901 atttcatctg aatagttatc ggcaagaaat ctttcaatgt tacctatgct taacggaggc
     3961 acagtccccc ctttcatttt tccgagcgta cattctttct gacactgatt ttcctgaggg
     4021 cacaccctgc cgcagatcgc ggggagaaaa ttcatctctc ttacggtttc aagtgcttca
     4081 gtaaatttct tttccgcagt cagatttaac attttcggaa tatcaattcc tacgggacat
     4141 cctttcacac aaggagcatt cttgcagtca agacaccttg atgcctcagt tacagccatt
     4201 tccatcgtat atccgagagg tacttcatcc atgttctttt ttcttttttc ggggtcgcat
     4261 aaaggcatta tctgtgccgg aatatcagtt tttttcattc gttcctctct tactgtgatg
     4321 atgcaattct gcaattatgg ttgtgctgat gtccgtatga ggaaagtctt tttataagag
     4381 aatcaaaatc aacaagatct ccttcaaact caggcccgtc aatacatgcg aattttgttt
     4441 ctccattcac agtgaccctg cacccgccgc acattcctgt gccgtctatc attatcgggt
     4501 tgagacttac tgttgaaggt atattgaatt tctgcagttc tttcacggtg gccgccatca
     4561 ttttaacagg tcccgctaca aatgctctgt caaaagggac atatttcttt accgcttctt
     4621 caataagtgc aataacgaat ccttttctgc cgatacttcc gtcatcagtt gaaatgtgaa
     4681 gtctgttgta tgaaagttca cgcatttcct cttcaagaac aagagtgttt ctgctttttg
     4741 tccctgcaag agttacacac gaaatgtccc tgtcatgaag atactgcatt accggaagaa
     4801 gaggcgctgc tccgataccc ccggaaatga aaagcacatt cttaagattt tcatcaattt
     4861 cggcaggttt tcccagaggt cctgccacat cgtttatgaa cgatccctct tcgcctgaac
     4921 atatttcctt tgtgccctct ccgatactct gaactatgag agttataaga ccgttctttt
     4981 catcattttt gcataccgtc aagggaatcc tttcactttg ttctgaaaaa cggacaataa
     5041 tgaattgacc cggacgccat gatcttgtaa tacgaggtgc ggtgataaca attttgtgaa
     5101 tgtcttttgc aattacagtg tttgaaatga tcttttccac aacaatactc cccgggtata
     5161 acccttttta aatagatttt gatatatagg aaaccaaccg gcacaaactg tttacataac
     5221 tacccatctc attgtcatac cacccgaata tcttcgcatg tgtaacaggt atatttgaca
     5281 aaggaggaat atcaatgaac ccggttcttg tgtgggtgtc gttactttca attacaactg
     5341 ccgacatttt tcctttcaag tcgcctgata cattctgcct ttgagaaaaa tggatgctgt
     5401 cgatgtattt accttcagcg gcatccttat atattctgtt tattacttct ctgcttattg
     5461 aagggaatcc gttgctgtca agcggggagt tgaatgtcag attaaggttt atgagtgata
     5521 cagtcataac aggaactctt accgaatccg ccataaatcc gatatttttc atttccggaa
     5581 gaatatattc aagtgcttta gccgctcctg ttgttgaaag aataatgttg ttccctgccg
     5641 cccggttttt tctaagatcg gatgttcctg aagacggaac tgaatcaaga acgctctgtg
     5701 aattagtcat tgaatggatt gttgacatgc ttgcggtgag aattgaagat gtctcctcat
     5761 ggtcaagaag gggctttatc atatgcgcaa gagccgttgt agtgcatgat gcagcagaga
     5821 tcacatggtg ttttgacgga ttaaatgact ggtgatttat tccgtatacc agcataactg
     5881 aatcgtccgg catgacttta ttctttccct taaatttaaa tggtgcggaa agaacaactt
     5941 tttttgcacc tgaaatgatg tgccccctca gatttcctga tgaagacggg tcttctgaag
     6001 ggtctgtgaa ttttcctgtt gtgtcaatga caatgcttac gccctccctg tcccagctta
     6061 ttttctcagg acttctttct tttcttaaaa atttaacttt ttttccgaaa aaaacggttt
     6121 ctccgttttc atcatcagtt atttctatgt tctcttttcc tttatagccg aaaaggaagg
     6181 attcaagtga gccgtaggtg gaatcgtttt taacataatc tataaggtct gacagagatt
     6241 ttcccgcctc tctgccgata tttataacaa ttttatcaaa atcgtctgac aatatcaggt
     6301 tccacaaagt caatttgccg attcttccaa gcccgttgat gccgatagcg tttttaaaat
     6361 tcatctgaat cattttttcc tccctgatac tataaaaaac cgtaaataag tccggactta
     6421 ccgtctatca ttatcttctc acccgcttta atggttatgc tgtttatatg gcacagcttt
     6481 ttttcttcat taagcacaag cgccctgcag tttacaacac ccgtcttgcc aagccgtgcc
     6541 gctgttactg ccgcatggga tgtcgctccg cctcttgatg ttaaaagccc gtctgcttca
     6601 aagattattc cgatgtcatc actcacagtg tcggggcgta tgacaattat tttttcttca
     6661 ggatatttct ttttaatttt catgagatcc tcttgtgaaa aagcggctct tcccataagt
     6721 gtatttccgc caatgcctgt tccttcgccg atctgagtgg cgttttcggg tattgaaaca
     6781 gaaagccacg gttcgttctg atgatactgt cttatctgca atatgtggag atctccgcta
     6841 ttcttggtct caaatgtgaa ttcaatttcc tgatgtccga accccttgtc actgacaagc
     6901 tgagaagaga tctttccaag ttctgaatat atttctggaa aatctttttc catgcttatt
     6961 tccggagcat ctttcattga aaaacgctgt ttttcagtaa cgggataagg gtgaacgaga
     7021 ccgcctacaa catcttctcc ctgactgcac atgacaaaat caccataaag ctttagatct
     7081 ctttcaccgg aaaaaggttc tcttgtaaaa ataacccctg tacctgagtc gtaattaatg
     7141 tttcccaaca ccattttctg aattattaca gcagttcccc attcaggcgc aatgttcatc
     7201 ttttttctgt aaacctcggc cctttctgaa tcccaggaat caaatactga atggatcgaa
     7261 cagataagct gctcttcaag gtcctgcgga aagaaaatat cagatacctc aaggattttc
     7321 atatattcct tagcgatctt tttcattacc gccggcttga attctatttt tttctcaact
     7381 ttataaattt tcttgaaatc agacattatc cggtcatata catccctgtt tgtgccgaaa
     7441 ctcatccccc agctctgaag aagtcttctg tagcaatccc acgatgtcca gccgtaatta
     7501 tcctttttag aaagtttttc agcaaactca tcattcattc ccacattaag aaaagtgttc
     7561 attgctcccg gcatggaaat cgccgatccg ctcctgaccg aaagtaaaag agggtttgat
     7621 ggatcaccgt atttcaaacc ggcacttctc tcaatttctt taagattttc catgatcatt
     7681 ttccggatct ctttttttat ctccggatgt tttataagtg ctttcctgcg tctgaaaagc
     7741 tctgtggtaa gtatgaatcc gggaggaaca ggcattccgt aacttcttat ttttttaagg
     7801 aaatatccct tcgcgccgag aaaaacctga ttgtccagtt catcgctctg ttcacaaagt
     7861 tttgatatta cagagtcgct tctgtaattc attatcaggt gtatgttctc gtttgaaata
     7921 tctgcgggca tttctctgaa cagggaaaga acattgttta taagattgtc aaccttctga
     7981 atgaggaagg atgacgaaat gacatctctg taaaattcct cggacctttt atgaatgtag
     8041 cattcctttt ccttttcatc agaaaatttc agatcgctct tttcaatgtg ctgctcaagt
     8101 atgagcttga gatttctatc atgtatcccc agaaaaaaat caacaactat ttttgaaata
     8161 tcctctgcca gaaactcaaa aatatttgta tactggttta tggaaaagct tgatgattcc
     8221 atgctgtatt taagtatgtt cagatcggat atgaggtttt cgttagttat tccgcccagc
     8281 tcaagccctt ctctgaacag atcaattatt tctgtaacaa tgctcagggt ttctgctgaa
     8341 atgtaattca gctttgcatt ggagatcatt ctgtccataa gcgatgaaac gaagatctcc
     8401 attctgaata tcatgccgag agcttcaaat tttggttcgc ggtattcccc gtacattgaa
     8461 ggaatgccta tggcgatatg tcttttatag tagatattct caaccccttc agtcttttct
     8521 gcggaaagca cggtgttttt aagatatgac attattgaaa aagaacaatt aagcgccttt
     8581 ttccaatttc ccgcagaaag ctcattttca agagattcga tcagctcttt aggtatatag
     8641 ttgaaatttc taagtttttt tgaaacatct cccggattga aataatattt ctctcttaaa
     8701 aggaaattca acttgaaaag atattcaact tttttaagtt cattgctttc aggaattttc
     8761 atggaatcga gcagtttaaa aagttcaccg gtatccatac tgaagagctc atctacatct
     8821 cctgagactt tttccagcac cgaggaggcg atctcgtgaa ttttaaggaa agagtgttcc
     8881 gaaatatcaa gagattcaaa aacatccaga ggaactctcc ccgccaggat ctcttttttc
     8941 gtgctgaccc agaaatcaaa aattattttt acgagctcaa tatgtgtgtt gttgctttcg
     9001 gcatggatct gctttctgag aaaatggatc agccggtcat tcctgtgaaa gagttcatct
     9061 atcgcagtgg atatttttct cagttcaccc tcagcaccga tctcattgaa atagaccgga
     9121 aacagcacag cgatctgtct tgcaatatga taggaagagc tgatcctgct gttaaggaat
     9181 tttgtgacat cgttttgaaa aaggtctgta tcggaaataa aaaccccgcc gactttaaga
     9241 ttgataagaa gtgcagaaag aagttttgtg cattttgaag ggtcgactgc aattatttca
     9301 agccaggtgc ggatattctt gatatggttc cggttcatta ttatctgcca gtcagaactg
     9361 acccctttca cttcgggata gacgaaccct gcttcgatca ccttttcaaa atagtggtcg
     9421 attaattcag gcccggcttt gcttacgatc tcaataccca gagtttttat gcagtctaaa
     9481 accgcactct ggaaacggtt ctgaaccggc ttaaaaaagt taaagatcct tgtgacaaat
     9541 aatttgcttt tttcattatc atcaagaaaa actgttttca gaatcctgtt aagatcaaat
     9601 agaaggtgct ctctcattcc tgccattgat tcaagtccga gaagatagaa aatgtaatat
     9661 attctgcttt caggagtgga tataagatcc aatgaacgac gcagattttc tgtgaaaaaa
     9721 gaaaaatcag gaacaacaag aagatcttcg aatgatttgg cgttatgtat catttcggca
     9781 gaatcttcaa acaactttga ggggaattga gtttttaaca tttcgtcaac ttcctttgat
     9841 tcaggaccgt attcaattcc caggccgtat tcccagacag caaagggttg tattgtcttc
     9901 cagtagagta tgttttttat cagagatttt ttcattatgt tgaaaactgt attctgcaaa
     9961 gccgcttgtg ttgccacacc ggccaaatat tttttcagat aactggatga ttcgatcagt
    10021 cccaaattgt ctgtagataa cattttatcg agtatatcaa catagtatgc caaagaagat
    10081 gttgctgttg tctcttgacc tgagaattta taaagaattt ccacagcgcc aagaagagta
    10141 ttgataacac gatgcagctg agactgattc agtttgaata atgttattga cactagttca
    10201 tctgcaaaac agatgaatat tttctctctt tcgggatgtt tcatgaaact ccagagattt
    10261 tcaagaatta tccatctgta accttcaacg atctcatcaa gatcgggaaa acggtggtgt
    10321 ttttctttaa ggtagtcggt caatcttttt gtgaccattg gaaagggggc gacaatttca
    10381 aggagaaaaa gttctttttc tgagaaattg ttatgaacag agtgtgtttc cagcatgtta
    10441 gctctaaggg cttctgattt gtgaacaata tttgccattt gtctgcttcc tttaaaaaga
    10501 aaaaacaaaa aaccttgcgc agccacttat atgcaaacga tatgccaggt aaatttaaaa
    10561 aacaaactca gttaattcaa ccacttagat ccggagctgc aaaattgatg gttttatgac
    10621 agggcatttt gccccatgtg ctctttttgt taatattttt tcagttttct tcttaaagtg
    10681 gtgatatcta ttccaagaag cattgcggct tttgttttgt tgccgtcagt gataagaagt
    10741 gccttttcaa tatattccct ttcgttatca gcaagtgaca tgaaattacg ggtctgtatg
    10801 ttctctccgg tatgttttaa agactcctga tttatgccgg atatatcgtc aagagtaatg
    10861 gcacttccct ttgaaagcag aacggctttt ctgataaaat ttcgtaattc ccttacattt
    10921 ccgtaccatg gtctgtcaca gagatattcc tgtgcctctt tagaaatatt atagacctgt
    10981 ttctttaatt cgtctgcggc ttctatcata aatcttgatg caagccatgt gacatcctct
    11041 tttctttccc gtaaaggggg gagaattatt tcaaattctg caagccggta gtaaaggtct
    11101 tccctgaatt ttccttcttt taccaactcg aaaagaggtt tgttgctggc actgattatc
    11161 cgcacatcaa tgtttgtagg ggtgtttgaa cccacaggaa cgatggtttt ttcttccacg
    11221 actctgagca gtcttgactg aaggtgtgcc gaagcattct caagttcatc aagaaaaatt
    11281 gttccgttat ttgcagattc aaaagccccg tatctttttt tatcggcacc ggtaaaagcc
    11341 cctttttcat gtccgaaaag ttcactttca ataagatttt cgggcaaagt tcccagatca
    11401 agtttaacaa acgggtgatt tttccttccg ctcatatcat ggatcgctct ggcaagataa
    11461 ctttttcctg agcccgtctc tcctcttaat attactgaaa aaggtgtcca tgcaacgctt
    11521 ctggctttgt caaaaacctc tttcatttta ggactttttg caaaaaacca gttttcctga
    11581 gagttctgtt cctgaagaga tcttatctgc tcctgctgca ttaaaggaag cgaaagatca
    11641 tacaggacat tgaggatgtt cttttcatca accggttttt gtaggtacct gtcaacacct
    11701 gcctctatgg cactgataag atcaggtatt tcagcaaatg cggtgttgag gatgaccgga
    11761 atgttcctgt tgatggcttt tatttccttt gtaagctgta ttccgtcttt ataaggcatt
    11821 ctaagatcgg ttattacaat atcgggacgg tgttgaatga atttttttaa tccgtcactt
    11881 ccgttagatg ccaggtgaat agtgtgaaag aaatccttaa gaaaaacaaa aacagtattc
    11941 ctcagatcat catcatcttc aacataaagc agttttatat tccgtaaaag aagaaattca
    12001 ctggggaaaa cacttttcat tacagcacct taacaaaaaa aaccgactgc tatataagag
    12061 atacagaata aaactgcaaa gtcaaataat acagaatttc agaaaccgga agggggcgtt
    12121 atgccctggt attttgccct gtctgtgctg aaatgattgg gaaaaaagac ttgccgcccc
    12181 tgtattaaca acataacggc attttggcac aaactttgct cagtataaga gttttaaggt
    12241 agaaaaagtt catttaattc ggagagtgat atgatgaaga aaaaagtgct tcttgttgat
    12301 gatgacgctg atgttgttgc tcagaacaaa atgttccttg aatctgccgg ttattttgta
    12361 gagtcggccg gaagtgcagg agaggcggaa aaaaaactgg attcatttga tccagacatt
    12421 gttgtggttg atataatgat ggagcacccc aaagccggag ttgagcttgc aagaaaactg
    12481 acagatccgg acaatgataa aagatatccg gttatatttc ttacaagcta cggagctaac
    12541 cctgagctgt tggatgaacc agattacata tggtcaaaaa cagtgaatgt cctgagtaaa
    12601 ccggccgatc ctttaaagct tttagatgag atcaacggat atttcgaatt tttaatgctt
    12661 caatccgatc aagtaaaggg ataaagaatt acactctcaa tctttacaat tttcaatact
    12721 tctgataaat taccatctgg ctgatttgaa atggaggaat tatgatctct aaaaagatgg
    12781 ctcttgacag cggtcttgca ctggtgctga tatcgctgat agtttttttc attactaaaa
    12841 acatttatgc aatttatttt gcaaccggtt ttactgttgt ctgcatgact gttccaattg
    12901 ttttcaaacc gtttgcaaag ctctggttcg gtctgtccca ttttatcgga acatttcttt
    12961 caaagatcat tctttcaata gttttttaca cagtgattac tcctgtggcg cttttaaggc
    13021 aggtgatggg caaagattca cttatgctta aaaagttcaa aaagggaact gattctgttt
    13081 ttgttgaaag gaatcatact tataaaagcg aagacatttt aaaaccatat taggagttag
    13141 caatggaatt tattaaagat ctctggggat ttttaagggt cagaaaaaaa tactggatga
    13201 ttccggccat ttttgttctt cttcttttcg gtgtgctgat cgtcctttca agcggcagtg
    13261 caattgctcc ttttatttac acaatattct aataaatccg aggtgttatg agacagatca
    13321 ttcttggaat atcggctttt tatcatgaca gtgcggcgtc acttttaatt gacggagaga
    13381 tcattgcggc ggctcacgaa gagcggttca caagaaaaaa acatactgcc gagtttccag
    13441 tgaacgcagt taaatatgtt cttgatgaag ccggaattgc tcttgatgat gtgacagtca
    13501 ttgcttttta tgataagcca tacatcaaat ttgagcgact tcttgagaca tatcacgcat
    13561 ttgcgccaca tggactcaaa agttttcttt cagccgtacc ggtctggata aaggaaaaac
    13621 ttttcatgaa agatatgctc aaaagcgaat ttgaaaagct ggggacaaaa atacctcaaa
    13681 tcgtttttcc tgaacatcat ctttcacatg cggcaagtgc tttttatccg tcaccttttg
    13741 ctgatgcggc gatacttact cttgacgggg tcggtgaatg ggcgacaaca acgatctgtc
    13801 acggaaccgg taaagatata aaaatactga aagaactcca ctttcctcat tcaatcggtc
    13861 ttttatattc cgctttcact cattactgcg gattcaaagt taattcaggc gaatacaagc
    13921 tgatgggact tgccccttac ggaaatcccg atagtgagga gcgtgagata ttcaagaaaa
    13981 agacacttga aaaccttgtt gatgtccgtg aagatggatc aatcctctta aatcagaaat
    14041 attttgatta tgcaacggga ctgagcatga ccaatgatga taagtggcag gaactgttcg
    14101 ggattccgag aagaaaatcg gaatcagatc ttaatcagag ttatatgaac atgtcattgg
    14161 cgattcagga gataactgaa gaagttgtga taaagctggc taaaaccgct aaagaattaa
    14221 caaaatctga taacatcgtt cttgcggggg gagttgcgct caactgtgtt gcaaacggaa
    14281 aactgcttag agaaaaaata ttcaagggct tatggatcca gcctgcggca ggtgatgccg
    14341 gtggatcgct cggagccgca cttgctgctt actacattca tctgaataac gaaaggacta
    14401 aaattgacaa acgggattcg atgaaagggg catatcttgg tcctgagttc ggtgatgttg
    14461 aaattgaaaa agtcgcaaga aaatatcagg cgaagttcgt taaatacgat gattttgaca
    14521 aactctgcga caaaactgcc gaccttttaa atggggatat tgtcatcggc tggtttcagg
    14581 gaaggatgga attcggccca agggcacttg gcaaccgctc tatccttgga gattcaagaa
    14641 gtccggaaat gcagaaaaaa ctgaacttaa agatcaaata tcgtgaagga ttcagaccat
    14701 ttgcgccatc agtcatggaa gaagaaataa gaactttctt tgatcttgat gacatcactc
    14761 cctatatgct tcttgtcgcc ccggttcaag aaaaaagaag aaataaagtt cctgcaaact
    14821 ataattctct gcctctttat gaacggcttt accatcttcg cagtgatgtg ccgtcaataa
    14881 ctcatgttga tttttcagca agaattcaaa gtgtcagcaa agagacaaac cttagatact
    14941 ggcagctcat tgacacattc agacagaaaa ccggcttcgg agtcatcgtc aacacctctt
    15001 tcaatgtcag aggcgaaccg atcgtctgca ctcccgatga cgcattccgc tgttttatga
    15061 gaaccgaaat ggattatctt gtcatgggca actatttctt tgacaaaaaa gaacagcccg
    15121 gctgggacaa aaaagacaac tggaaagaag aattcaaact ggattgagga tctg
//
LOCUS       DAOOYB010000047        15139 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_3930_length_15139_cov_9.173296, whole genome shotgun sequence.
ACCESSION   DAOOYB010000047 DAOOYB010000000
VERSION     DAOOYB010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 15139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15139
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3930_length_15139_cov_9.173296"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1080
                     /locus_tag="PL056_04345"
     CDS             <1..1080
                     /locus_tag="PL056_04345"
                     /inference="COORDINATES: protein motif:HMM:NF028240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HPM46274.1"
                     /translation="KKEKNILDMFSLNGKIKTRYEFEKKAKIDRSGHDTRHRGVFEAK
                     LGFVFRPVDYFKAGFGLASGAETPVSSNETMKGQASSKPIFLDLAYIEVHPYYKWISL
                     LTGKFRNPLFKAGGSQMVWDNDVNPEGAALSFKYDFGQFNLFGSGALHILEEFKVEPD
                     DPFMAVLQLGAGLNISGVKFNVAVSYSDTINLEKKLTQSRAGSDSDYSYSYSNSVSLD
                     DKYVYDYNVLGVDADFSYTAHKYFMITLFGQFYSNVAKDVEERYAYIYGIKISSEKMK
                     KPGDYCFEFRSRHVDRDAYLDSFTDGTFYYGRTGIVSYDLAVKAAIWNDVTFDVLYSR
                     QMLKTTDGVYNQPIDSVQLNFEVKF"
     gene            1119..2069
                     /gene="trxB"
                     /locus_tag="PL056_04350"
     CDS             1119..2069
                     /gene="trxB"
                     /locus_tag="PL056_04350"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HPM46275.1"
                     /translation="MFFRRMNMSAVYDVAIIGAGPAGMTAGIYASRAMLKTVMIEKGV
                     AGGLMALTDKLDNWPGELSVSGAELSEKMLKQATGFGCEFQMGEVTKIDKLSDGNFVI
                     DLADNEKILAKTVIYAAGSVPRLADIEGEKEFAGRGVSYCAVCDAAFYRGLKVAVLGG
                     GDSALKEALYLTKFADEVTIIHRRQEFRAEKIIQEEVKNNPKIKFVLDSIAEKIEGSS
                     FVERVVVKNVKTGEMSVVNADGVFVFLGYIPKVDPVRHFVELAEDGRIKADYRMHTNV
                     EGFFAAGDVIQKLVNQVATAVGDGATAATAAEHFISSAKK"
     assembly_gap    2144..2243
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2244..2438
                     /locus_tag="PL056_04355"
     CDS             <2244..2438
                     /locus_tag="PL056_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine dimethylase"
                     /protein_id="HPM46276.1"
                     /translation="VGGDVGGGESGIVHTVPLYLKEFNIVTVYAHGVFAVDSFDFNGP
                     LKSIWKLESLCAKRYIEKYL"
     gene            complement(2435..3595)
                     /locus_tag="PL056_04360"
     CDS             complement(2435..3595)
                     /locus_tag="PL056_04360"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012342913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HPM46277.1"
                     /translation="MNNFWLTLPTEIFFGKGQISVLGKTLKKEGCTKALLVYGYGSIK
                     RNGLYNDTVNELNSHGIPFVELQGVDPNPRISSVRKGAELCRKHNIDFIIAAGAGSVI
                     DCCKAIAAARYYNGDPWDFFIRRTSVKKALPICSILTLAATGSEMNGGTVITNELTGE
                     KLSTGSNKMKPRFSILDPVYTFTVPSHQTAAGVADIMSHVFEQYFSYPEAYLQDRMSE
                     AVLKTVIKYGPVVIKEPENYEARANIMWAGTIGLNGLLGIGKDGDWATHLIEHELSAK
                     YDITHGTGLAILTPHWMRRVLDTENASKFAEYGRNVWNILDKDDMSCAVAAIDRTAVF
                     LTSIGIPSKLSELGIDDSKFEEMAGSALKYSKIGRFKSLSKEDIVDIYKAAL"
     gene            complement(3597..4496)
                     /locus_tag="PL056_04365"
     CDS             complement(3597..4496)
                     /locus_tag="PL056_04365"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPM46278.1"
                     /translation="MSQIKIYSFALISMTFWALSFVWYKDAFISYGPVTIIFFRLILS
                     VSLLLIVTRIIRKDLRLEKKDYRYMALLSFFEPFCYFLGEGFGMQHVSPTTGAIIIST
                     IPLITPFVLMLMNLKETIHFKNFLGMMISFAGIILVVTSDNASFAASLKGVSLLFVAV
                     ISAIFFSIILKKVGNRYNSMVIVFWQNIFSVLYFMPLFLALELESFMASSHEPAAYLA
                     IIKLGTFPSTISFILFIPVVTYLGATKANAFANIIPVITAIFSYFYLGEQFSLLKILG
                     VAAVITGLFFSQMRFIKTKIAEV"
     gene            complement(4521..4799)
                     /locus_tag="PL056_04370"
     CDS             complement(4521..4799)
                     /locus_tag="PL056_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015837447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPM46279.1"
                     /translation="MNRTVLSKEIAKKKGVSQKEALKYIDLITESIVNAMEKGERVEI
                     RGFGSFIVKKYDKRTGINPKSGEKITIPAKKLPSFKPGKDLKSKINSK"
     gene            complement(4809..5216)
                     /locus_tag="PL056_04375"
     CDS             complement(4809..5216)
                     /locus_tag="PL056_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46280.1"
                     /translation="MRSVFFIAAILLFSLPVSAAAAFAEAIDKDGNSITWTFSFEDGK
                     NVTKAKNNAIAQLKKEGHSSVRSAASTTLNQGFFAVLYSAYWVDGILKKTFAFAFSSK
                     SEEDAKNEALKNLKKIKGWKEEQGYSVNKSGSF"
     gene            complement(5293..7122)
                     /locus_tag="PL056_04380"
     CDS             complement(5293..7122)
                     /locus_tag="PL056_04380"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HPM46281.1"
                     /translation="MEENKQGTSKPTIDKEYLKKLAEKIKAEKARKESLQKKPENEST
                     TPPPPPMASISPTLLDDDDEEEDMAAEKTAIIDLTALSGHTADARLTILDGKDEGKSI
                     DITRDEIFAGRSLDNDFVISDISVSRKHFKVMREGDHFSVSDMGSGNGIRVNGVKQPV
                     TTLYHGDVITAGARQIKFEILNPDMKEKYSRKSDVPDVQEAVYSKEKRKGSSILPWVA
                     IFIVISGLAAGGYLFMKQSEQIQQVAAAEFKLGIEDIDKIDEMIEQKDLKGAEKNTKF
                     FLEKVPGNKQLSERLVLIEKEKAVQTKFEQGKVIFTQNKDEASKILKEIPEDSIFYED
                     VKTLVGPEMIIKWLVDDIKALISAKKEDDALKKIYAALIENPDNEEIKNLQKTITSQL
                     GEEKAKDIEKKAAEEKKAAEEKKAAQKKKQEERQAKRRPAPRPQPKPQPVEKVDTSSG
                     GSESDLERAIELYLSKDFDGAIQNLEKAATSRDKGISKKAQLLKLEVTKFRKAWPAVQ
                     SEKGKKKKSTIKKLVIYDKKISGGQLASEIEALDSDESQASSSRSKSSGMSDDEAKEL
                     YMRARSMRNEDPSKARELLQQVIDGTDSSNDYNIKSKKLLKNL"
     gene            7309..8529
                     /gene="coaBC"
                     /locus_tag="PL056_04385"
     CDS             7309..8529
                     /gene="coaBC"
                     /locus_tag="PL056_04385"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HPM46282.1"
                     /translation="MVKNNILKGKKIVLGVTGGIAAYKSVYLLRLLVKNGASVQVVGT
                     LNSLNFIGKATWESLSGRMPLFDTFENSDSSKISHIILAQEVDAVVVAPATGNIIGKA
                     ACGIADDLLSTILMAASVPVLFAPGMNTVMYENPATICNIGTLSKRKNIFFVEPQTGE
                     LACGTSGKGRMAEPVDIIKKLEEILSPVGSSGVKWLVTGGATREFIDPVRYITNGSSG
                     RTGIAIAEAAFRSGGDVTFVGVNVETSLSRSIKKIKTVTAAQTSTAVKDLVKESDIFV
                     MSAAVADYTPDKNRSKIKKKEDGLVIELSRTEDILQATVDMMKQGSVRIGFAAETENL
                     AENALLKLRKKKLDMVVANLVSEEFDPFGSETNSVTMITESGTESFENIDKHELGRII
                     VEKAMQIFAVKNGK"
     gene            8519..8953
                     /locus_tag="PL056_04390"
     CDS             8519..8953
                     /locus_tag="PL056_04390"
                     /inference="COORDINATES: protein motif:HMM:NF014762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HPM46283.1"
                     /translation="MENNPSSLKKLLTATQEDYLEAVLNLTSSRKVARNMEIADLLGV
                     KRATVTRAIKLLAKKGLVEHETHGYVTLTELGRSIAVEITERHSLFTHFFKDILGVSQ
                     SESDKLACKIEHMISGRVLQKFRDFIEKTDAAVNSENNRKRK"
     gene            8959..9633
                     /locus_tag="PL056_04395"
     CDS             8959..9633
                     /locus_tag="PL056_04395"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HPM46284.1"
                     /translation="MERSYQPGETVLIKNELSGNFFIIIRGRIKFELNGKRFFLSDGD
                     FFGEEGVFFNKPNPFSVTAAEETQVQIFDRNEAKNFISHNHDAAFNMFIRNSARFSEG
                     IEPVSDLSHVHIKILERITPFVSHSEGDFPEYEATTDIEKFSTMLQMTTDKMVNLLET
                     VEPLGYVKLSSKGKILTSGKSRINKLIRRYYREMVFYGAEAGSGSGLFPLINVINKKE
                     KKGINI"
     gene            9654..13367
                     /locus_tag="PL056_04400"
     CDS             9654..13367
                     /locus_tag="PL056_04400"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPM46285.1"
                     /translation="MKIKCPHCNTNYNIDDSKIPEKGLLVKCSVCKNQYRIKKQEESA
                     ADDDVVGKMHEKMDIETGRYDHDQSNPSPEKSDRVNVNKDDFLEDEETKEDTLDFKIK
                     TPPQEPEKTVPPVEKRKDDNEFSLSNDRFDGDSLFEDKPSDKLKKEDHLSLFDDDPDE
                     EIAGEPVKTADSSDLFSDAKSPETKVERGPAEKSSKPGQDDFLKELFGDAKTGFEEEE
                     EKKGIYFRKKSTGEVVGPFDEAEIENLMLNAVISEEDDISYDGFSWSSSDGSLQPKTE
                     SKSSPRYGLSLDDEEGEAFDSISFDQDRGISETGVFKDAANEFEDTSFTSVGGQNQNV
                     DHIFIPDDFSEDKMEKTGHQKKSKKERKGDSPVLFYAVLSFSTMIVLGLIGGGFYYYL
                     KYVKGTQGDILDNISESIAVNTGTLVDVRDALNKDLPDDYINSIGILKQYLKSEDSAP
                     SAVGLDGQVKFNLLISYNRRLEPSATLNEKIDAALKKAPANLDLIKAKALSLYESKMF
                     DEALVLIQPFAESSDQEIFYILGLIAQAKKDVQKAEMFFNQGFMQGGGKNSKIIYALA
                     EMKYRNGDAQSAMAFLNRIISDNPNYLKAYLFKAGIIMNTDNKLEEAEKFLKGVSTMI
                     ISKAEDFQKADYYQKLATIAHKRGLMKEAVGYYEKAVEINKTDTSSIATIADFYVQIS
                     NSSKAMEYYEMALKIDPKFPPAILGKTEIFILLGQNDKVYLELAKLDIKSVVDAQFLI
                     RLGRIYYKIGDKGKSMEFYDLAIKSNPSLVEPYLAKVVILLEFKKIKEIDAIAAVVEK
                     LGKETYAFNLIKAVVQHEEANYKRAEEYFKKAVERNTAGDDRVFFYYGLFLFDQQNYR
                     ESSKMLEKAYKIDPRNYNYIQSYADSLEKEKKWKAVIDLLETGTFNEKRMYRSFVTLA
                     NAFYHLNKFDEALNFVNRAIELNNQHTHVFHMKAKIYYAMEKYNEAEKEIDTAVVIDM
                     RNFDNYMLYAKVLAKKGDFKGAIEKIEAAEKIDPSDQQLMLMKGIIYKNLDDYRSALA
                     YFRKVTNKSFIKEAYLEIGESYLQLNRVDDAMKFFKKAENSGNKLAHKHLARIYYESG
                     KLDQAVTYYRKALRADKNDTTAMRQLGYIYKEKQEWSKALSYLKMYQKRINDAAEKKM
                     IDEEVYFLEKNLPQGRPVHIAESLNELDENDSEAINERAKELYIEGRALRDEDPETAR
                     DRFREVMKIVPKTNEYYKKAFKAFNKLNESKEK"
     gene            13507..14685
                     /gene="rocD"
                     /locus_tag="PL056_04405"
     CDS             13507..14685
                     /gene="rocD"
                     /locus_tag="PL056_04405"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HPM46286.1"
                     /translation="MHIEHTYGAHNYHPLEVVICKAEGVWVWDPEGKKYMDCLSAYSA
                     VNQGHRHPKIIKALKDQADILTLTSRAFFNDKWPLFAQKLAEYTGKDMILPMNTGAEA
                     VETAIKMTRRWGYEVKGVPADKAEIIVMKENFHGRTTTIVSFSTDPDAKNNYGPFTPG
                     FKVVEYNDLKAIEEAITPNTVGVMLEPIQGEAGVFVPFDGYLKGIREICDKHNVLFVC
                     DEVQTGFCRTGKKFAFMHEGIVPDVIIMGKALGGGIMPVSAVAANKDIMGVFTPGTHG
                     STFGGNPLACAVATAALDVLEEENLAQRSEELGKYFRERLVAMNSPKIELVRGKGLLN
                     AVVLKKEAGKARIYTTAMKNRGVLAKETHEWIIRFAPPLTVTKEELDWALNIIEEVLK
                     "
     gene            complement(14701..>15139)
                     /locus_tag="PL056_04410"
     CDS             complement(14701..>15139)
                     /locus_tag="PL056_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46287.1"
                     /translation="TITIFVFLLFSASCGSTEKKKDPTIKGARADSGITVKDENFKFE
                     GIKKVILYNEVDDEESNAILYGISGAFQQKGIEAVIDTGESMTYDGESFRVRTYKNLM
                     SVYFNLVILKPGGKKGNEEIIADIKADSIPKLVEITVDFIFQK"
BASE COUNT         4583 a         2788 c         3292 g         4376 t
ORIGIN      
        1 aaaaaagaga aaaacattct cgatatgttc tctctgaacg ggaaaataaa aacaaggtat
       61 gaatttgaaa agaaggcaaa aatagacaga tcgggtcatg acacccgcca cagaggggtt
      121 tttgaagcaa agctgggatt cgttttcaga ccggtggatt attttaaggc gggtttcggg
      181 cttgcatcgg gagctgaaac ccctgtttca tcaaatgaaa cgatgaaagg acaggcatca
      241 tcaaaaccga tattccttga tctggcctat attgaagtac atccgtatta taagtggata
      301 tcacttctca cagggaagtt cagaaatcca ctttttaaag ccggtggaag ccagatggtg
      361 tgggataatg atgtgaatcc tgaaggagcg gcactcagct ttaaatacga tttcggtcag
      421 ttcaatcttt tcggaagcgg agctctgcat atccttgaag agtttaaggt cgagccggat
      481 gaccctttca tggctgtttt gcagcttgga gcaggtttga atatctcagg tgtaaaattt
      541 aatgtcgcag tgagttattc agatactata aatcttgaaa agaagcttac ccagtcaaga
      601 gccggctcag actcggatta ttcgtattct tattccaact ctgtttctct tgatgacaaa
      661 tatgtttatg attacaatgt gttgggggtt gatgctgatt tttcatacac agcacacaaa
      721 tacttcatga tcactctttt tggacagttc tactcaaatg ttgccaaaga tgttgaagag
      781 agatacgcat atatctacgg aataaagatc tcaagcgaaa aaatgaaaaa accgggtgac
      841 tactgttttg aattcagatc aagacatgtg gacagggatg catatctcga ttcttttact
      901 gacggcacat tctattatgg aagaacaggt attgtttcgt atgatcttgc agtcaaagcc
      961 gccatctgga atgatgtgac ttttgatgtt ctttactcaa gacagatgct taaaaccaca
     1021 gatggtgttt ataatcagcc gattgattca gttcaactca attttgaagt aaaattctaa
     1081 gttgactctt ttttcattgt gcttattcta cagtgagcat gtttttcagg aggatgaata
     1141 tgagtgcagt ttatgatgtg gcgattatcg gtgcaggtcc ggcagggatg acagcgggga
     1201 tatacgcttc gagggcgatg cttaagactg tcatgatcga aaaaggtgtt gcaggcggat
     1261 tgatggctct gaccgataag cttgacaact ggccgggaga gttgtcggtt tcgggagcag
     1321 agctttcaga gaaaatgctt aagcaggcga ctggtttcgg gtgtgaattt caaatgggag
     1381 aagtaacaaa aatagacaaa ctcagtgacg gtaattttgt tatagatctt gctgataatg
     1441 aaaaaatatt agcaaaaaca gttatttatg cggccggctc tgttccaaga cttgcagaca
     1501 tagagggtga aaaagagttt gcaggcagag gagtaagcta ttgtgcagtg tgtgatgcgg
     1561 cattttaccg cggtcttaaa gtcgccgttt taggtggcgg agacagtgca ttgaaagagg
     1621 cactttatct tacaaaattt gctgatgaag ttacaataat tcaccgtagg caggagttcc
     1681 gggctgaaaa gataattcag gaagaggtga aaaacaatcc gaagataaaa tttgttcttg
     1741 acagtatcgc tgaaaagata gaagggagca gttttgttga aagagttgtc gtgaagaatg
     1801 ttaaaacagg ggaaatgtcg gtggttaacg ctgacggggt gtttgttttc cttggataca
     1861 ttcccaaagt cgatccggtt agacattttg ttgaacttgc tgaagatggc aggattaaag
     1921 ctgattatag aatgcataca aatgtcgaag ggttctttgc ggcaggcgat gtgattcaga
     1981 aacttgtaaa tcaggtcgca acagctgtcg gtgacggtgc aactgcggcc actgccgctg
     2041 aacattttat ttcatcggct aaaaagtaga gtttacgatt gtcatattta atagacccga
     2101 aactcaaaca ggcaaaagtt cttgctgaat atgctttaac aggnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnngttggag gagatgtcgg agggggagaa agcgggatag
     2281 ttcataccgt accgctctat ttaaaggaat tcaatattgt aacagtctat gctcacggag
     2341 tttttgcagt ggattccttt gattttaacg gaccgctgaa aagtatttgg aaacttgagt
     2401 cactgtgcgc aaaaaggtat attgagaaat atctttaaag agcagctttg tatatgtcaa
     2461 caatgtcttc tttcgaaaga cttttaaacc gcccgatctt tgaatatttc aatgcggatc
     2521 cagccatttc ttcaaacttt gagtcgtcaa ttccaagttc agagagcttt gaaggaattc
     2581 cgatggatgt gaggaaaacg gcagttctgt caattgccgc aacagcacac gacatgtcgt
     2641 ctttgtccag gatgttccag acatttcttc cgtattcagc aaattttgaa gcattttctg
     2701 tgtccagaac ccttctcatc cagtgaggcg tgagtatcgc aagacctgtt ccatgcgtga
     2761 tgtcatattt tgcactcagt tcgtgttcaa taagatgtgt cgcccagtca ccgtctttgc
     2821 cgattcccag aagtccgttc agaccgattg ttcctgccca cattatattt gcccgtgctt
     2881 catagttttc cggttctttt attacaacag gcccatattt tataactgtc ttcagcacgg
     2941 cttctgacat tctatcctga agataagctt caggataact gaagtactgt tcaaatacat
     3001 ggctcatgat atcagcaact cccgctgcgg tctgatgtga aggaacagta aaagtgtaaa
     3061 cagggtcaag aatactgaaa cggggcttca ttttgttact tccggtggaa agcttttccc
     3121 cggtaagctc gttagttatg acagtaccgc cattcatttc acttcccgtt gctgcaaggg
     3181 tgagaattga gcatatcggc agagcttttt ttacggaagt tcttctgata aagaaatccc
     3241 atggatcacc attgtaatat cttgctgcag cgattgcttt gcagcagtct atgacacttc
     3301 ctgcccccgc tgcgattata aaatctatgt tgtgtttcct gcaaagctca gcaccttttc
     3361 ttacactcga gattctcggg ttggggtcaa ctccctgaag ttcaacaaac ggaatgccgt
     3421 gtgaattcag ttcattaact gtgtcgttgt ataatccgtt ccttttaatg cttccgtatc
     3481 cgtaaacaag aagggctttt gtacagccct cttttttcag agtttttcca agcacgctta
     3541 tctgcccttt gccgaagaat atttcagtcg gaagggtaag ccagaaatta ttcatatcag
     3601 acctccgcga tctttgtttt tataaatctc atctgagaaa agaaaagtcc cgtgataaca
     3661 gcggcaacac caaggatttt tagtagagag aactgttctc caagataaaa atatgagaaa
     3721 attgcagtta ttacaggaat aatatttgca aaagcatttg cttttgtggc accaagatat
     3781 gtaacgacag ggataaagag aatgaatgaa atagtagacg gaaatgttcc aagctttatt
     3841 attgcaaggt atgcggcagg ctcgtgtgaa gaagccatga aactctctaa ttccaaagca
     3901 aggaacagag gcatgaaata taatactgaa aatatattct gccagaaaac tatcaccatt
     3961 gaattatatc tgttcccgac ctttttaagt atgatcgaaa aaaagatagc actgatcaca
     4021 gcaacaaaaa ggagggaaac accttttaaa gatgcagcaa atgaagcatt gtcgctggtc
     4081 acaacgagaa ttatgccggc aaaagagatc atcatgccaa gaaaattctt gaaatgtatg
     4141 gtttccttaa ggttcatcag cataagcaca aacggagtta tgagaggaat tgttgaaata
     4201 attatcgccc cggttgtagg ggacacatgc tgcattccga aaccttctcc cagaaaataa
     4261 cagaagggtt caaaaaaaga gagaagagcc atatatctgt aatccttttt ttcaagacgg
     4321 agatcttttc tgattattct tgtcacgatc agtaaaagag atacagataa aataagacgg
     4381 aaaaatatta tcgtaacagg tccgtaactg atgaaagcat ctttatacca gacaaatgaa
     4441 agtgcccaga atgtcatgga tataagtgca aatgaataaa tctttatctg actcaaactg
     4501 aattctccgg aaaaaaagga ttatttgctg ttgatcttgc ttttaaggtc tttgcccggc
     4561 ttgaaagaag gaagtttctt cgcggggatt gtgatcttct caccgctttt cgggttaata
     4621 ccggtccttt tgtcatattt ctttacaata aaactgccga aaccgcgaat ttcaactctt
     4681 tctccttttt ccattgcgtt aacaatgctt tcggttatga gatcaatgta tttgagggct
     4741 tccttctgcg aaacaccttt cttttttgcg atctcttttg ataatacggt cctgttcatt
     4801 gctgcctcct aaaatgaccc tgatttattg acgctgtaac cctgttcttc tttccatccc
     4861 ttaattttct tcaaattttt cagtgcctcg ttctttgcat cctcttcaga tttcgaactg
     4921 aatgcgaatg caaaggtttt tttgagtatt ccgtcaaccc agtatgctga ataaagaaca
     4981 gcaaaaaatc cctggttcag agttgttgac gcggcacttc tcacacttga gtgtccttct
     5041 tttttcaatt gtgcgatggc attgtttttc gcttttgtaa catttttccc gtcttcaaag
     5101 gaaaatgtcc atgttattga gttcccgtct ttgtcaattg cttcagcaaa agccgccgct
     5161 gcggaaacag gaagagaaaa cagtaaaatt gctgcaataa aaaaaacaga tctcatgaaa
     5221 acctccatat aaaaatcata aatacagaca aatgatgcca tctaagaaaa aaaagtcaaa
     5281 gtaatttaag gatcagagat tcttcagaag tttctttgat tttatattgt aatcatttga
     5341 tgagtcggtg ccgtcaatta cctgctgaag taattctctt gcttttgacg gatcctcatt
     5401 tctcatgctt cttgctctca tgtagagttc ttttgcttca tcgtcactca ttcctgaaga
     5461 tttagatctg gaagacgatg cctgtgattc atcactgtca agagcttcaa tttcagaagc
     5521 aagctgcccg ccggagattt ttttgtcata aattacaagc tttttaatag ttgatttctt
     5581 tttctttcct ttttctgact gaactgccgg ccatgctttt ctgaatttag taacttcaag
     5641 ttttaaaagc tgagcttttt tcgaaatccc tttgtctctt gaagtagcgg ctttttcaag
     5701 gttttgtata gcaccgtcaa aatcttttga aagataaagc tcaatggctc tttcaaggtc
     5761 gctttcagag ccaccggaag aagtgtcaac tttttctaca ggctgaggtt tcggctgagg
     5821 tctcggagcc ggacgccttt ttgcctgtct ttcctcttgt ttctttttct gggcggcttt
     5881 cttttcttct gcagcttttt tctcttcggc tgctttcttt tcaatatcct ttgctttttc
     5941 ttcaccgagt tgtgatgtta tggttttctg gaggttctta atttcctcgt tatcaggatt
     6001 ttcgatcaga gcggcataga tcttttttaa cgcatcatct tctttttttg cagaaattag
     6061 agcttttata tcgtcaacca gccattttat gatcatttct gggccgacga gtgttttaac
     6121 atcttcatag aaaatggaat cttcagggat ctctttaaga atttttgaag cttcatcctt
     6181 attttgagta aaaatgactt taccttgttc aaactttgtc tgaacggctt tttctttttc
     6241 tatcaagaca agtctttcag agagctgttt gttgccggga actttttcaa gaaagaactt
     6301 tgtgttcttt tcggcacctt tcaggtcttt ctgttcgatc atttcatcga tcttatcaat
     6361 gtcttcgatc ccaagcttga actctgctgc ggcaacctgc tgtatctgct ctgactgctt
     6421 catgaaaaga tatccgcctg ctgcaagacc gctgataact atgaagatgg caacccatgg
     6481 aagtatggaa gatccctttc ttttttcttt agaataaaca gcttcctgaa catcgggcac
     6541 atcactcttg cgactgtatt tttctttcat gtcaggattg agtatttcaa attttatctg
     6601 tctggcgcct gctgtaatga catcaccgtg gtaaagggtt gttaccggct gtttcacccc
     6661 gttaaccctt atgccgttac cgcttcccat gtctgaaacg gaaaagtgat ctccttcacg
     6721 cattactttg aaatgctttc tggaaacgga tatatcgcta ataacaaaat cgttgtccag
     6781 acttcttcct gcaaatattt catctcttgt tatgtcaata ctcttgcctt catccttgcc
     6841 gtcaagtatt gtcaatcttg cgtccgcagt atgtccggaa agtgcagtaa gatctattat
     6901 cgctgtcttt tctgcagcca tatcctcttc ttcatcgtca tcatcaagaa gagtcggcga
     6961 aattgaagcc atcggaggcg gaggcggtgt ggtactttca ttttccggtt ttttctgcag
     7021 tgactccttt ctggcttttt ccgcctttat cttttctgca agtttcttga gatattcttt
     7081 gtcaatagtc ggcttactgg ttccctgttt gttttcttcc ataatctaaa ctcctgaata
     7141 gaacaacaca aaaaaaatgt cataatttga tagtgcaaaa aaacttaaaa gtcaattatt
     7201 tatacaatta aaggttgatt tatatgttga taaatttttt gtgtattatt ttttcggtgc
     7261 tataatgtca cgcttattgt tttaatgatg gttggcttaa aggttcagat ggttaaaaac
     7321 aacatattaa aagggaaaaa aatagtgttg ggcgtaacag gtggcattgc agcatacaaa
     7381 tcggtgtatt tattaagact cctcgttaaa aacggtgctt ctgtccaagt tgtagggacc
     7441 ttgaattctt taaacttcat tggaaaggcg acatgggagt ctctttccgg aagaatgccc
     7501 ctttttgata catttgaaaa cagtgattct tccaaaataa gccatataat tctggctcag
     7561 gaagttgatg ccgtagttgt tgctccggca acaggaaata ttatcggcaa ggcggcatgc
     7621 ggaatagcgg atgatcttct ttctacgata cttatggcgg cttcggtgcc ggtgcttttc
     7681 gctcccggaa tgaatacagt gatgtatgaa aatcctgcga caatatgcaa catcgggaca
     7741 ctcagtaaaa gaaagaacat tttctttgtg gaacctcaaa ccggtgaact tgcctgcgga
     7801 acatccggaa aagggcggat ggcagaacca gttgatataa ttaaaaaact tgaagagatc
     7861 ctgtcgcccg tggggtcatc aggagtaaaa tggcttgtca caggcggtgc gacacgcgaa
     7921 tttatagacc cggtgagata tattaccaac ggatcttccg gccggacagg tattgcgata
     7981 gcagaagccg ctttcagatc aggaggcgat gtgactttcg tgggtgtaaa tgttgaaaca
     8041 tctttgagcc gcagtataaa aaagataaaa accgttacag ctgctcagac ttcaactgca
     8101 gtgaaagatc ttgtaaaaga atcggatatc tttgtgatga gtgccgcagt ggcggactat
     8161 actcctgata aaaaccggtc caagataaag aaaaaagagg atggtttagt gatagagctt
     8221 tcgaggacag aagatattct tcaggcaacg gttgatatga tgaaacaggg ttctgtccgt
     8281 ataggttttg ctgctgaaac tgagaatctt gcagaaaatg ctttgttgaa actgagaaaa
     8341 aagaaacttg atatggttgt tgccaatctg gtgtcagaag aatttgatcc tttcggttca
     8401 gaaactaaca gcgtaacaat gataactgaa tcgggaactg aatctttcga aaatattgat
     8461 aaacatgaac tcggcaggat cattgttgaa aaagccatgc agatttttgc ggtgaaaaat
     8521 ggaaaataac ccttcatcat taaaaaagct tcttaccgcg acgcaggaag actaccttga
     8581 ggcagtgttg aatctgactt ccagcagaaa ggttgcaagg aacatggaga ttgctgatct
     8641 gctcggagtc aagagggcta ctgttacaag agccataaaa ctgcttgcaa aaaaaggtct
     8701 tgtcgagcat gagacacatg gatatgtaac cttgacagag cttggcagaa gcatagctgt
     8761 cgagataact gaaagacaca gtttgttcac tcattttttc aaagatattc tgggtgtgag
     8821 ccagtctgaa tctgacaaac tcgcatgtaa aatagagcat atgatatcag gtagagtgct
     8881 tcagaaattc cgtgatttca ttgaaaaaac agatgccgct gtaaattcag agaacaatag
     8941 gaaaaggaag taaaagaaat ggagagatct taccagccag gagagacggt actgataaag
     9001 aatgagcttt ccggaaactt tttcataata ataagaggaa gaataaaatt tgaattgaac
     9061 ggtaagagat tttttctttc agatggtgat tttttcggag aagaaggtgt ctttttcaac
     9121 aaacccaacc ctttttcggt tactgccgct gaggaaactc aagtacagat atttgacaga
     9181 aatgaagcga agaactttat ttcgcacaat catgatgccg catttaatat gttcataaga
     9241 aattctgccc ggttttctga aggtatagag ccggtttcag atctgagcca tgtgcacata
     9301 aagatactcg aaaggatcac tccttttgtt tctcattcgg aaggggattt tcctgaatat
     9361 gaagcaacca cagatattga aaaattttcg acgatgcttc agatgaccac ggataagatg
     9421 gtgaatcttc ttgaaactgt tgagccgctg ggttatgtga aactcagctc caaagggaag
     9481 atcctgacat caggtaagag cagaataaat aaattgataa gaagatatta cagggaaatg
     9541 gttttttacg gtgctgaggc aggcagtggg tcgggtcttt tcccgttgat aaatgtgatc
     9601 aacaaaaaag agaagaaagg tattaacata taaacttttt aatgggtctg aaaatgaaga
     9661 tcaaatgtcc tcattgcaac actaactata acattgatga ttcaaaaatc cccgaaaagg
     9721 gtctgctggt gaagtgttcc gtatgcaaga accagtacag gatcaaaaaa caggaggaga
     9781 gtgctgcaga cgatgatgtc gtcgggaaga tgcatgaaaa aatggatatc gaaacagggc
     9841 gatacgatca tgatcagtca aatccctcac ctgaaaagtc tgacagagtc aatgttaata
     9901 aagatgattt tcttgaggat gaggaaacaa aggaagatac tcttgatttc aagataaaaa
     9961 caccgcctca ggagccggaa aaaacagttc ctccggtaga aaaaagaaaa gatgacaatg
    10021 aattttctct ttctaatgac aggtttgacg gtgactccct ttttgaagat aaaccatctg
    10081 ataaactgaa gaaagaagac cacctttcgc tttttgacga tgatccggat gaagagattg
    10141 ccggggaacc tgttaaaacg gccgattctt cagatctttt cagtgatgca aaaagtcctg
    10201 aaacaaaagt tgaaagaggt ccggctgaaa aaagttcaaa gcccggccag gatgattttt
    10261 taaaagagct tttcggcgat gcaaaaacag gatttgaaga agaagaagag aaaaagggga
    10321 tatatttcag aaaaaagagc accggtgagg ttgtgggacc ttttgatgaa gccgagattg
    10381 aaaatcttat gcttaacgcg gtcatatcag aggaagatga catcagctat gacggttttt
    10441 cgtggagttc aagtgatggt tcgctacaac cgaaaactga atcgaaatct tctcccagat
    10501 acggcctttc tcttgatgat gaggagggtg aggcgttcga ttccataagc tttgatcaag
    10561 atcgggggat ttctgagaca ggtgttttca aagatgccgc caatgagttt gaagatactt
    10621 cgttcacaag cgtcggcgga caaaatcaga atgttgatca tattttcatt ccggatgatt
    10681 tttctgaaga taagatggaa aagacaggtc atcagaaaaa atcaaagaaa gagagaaaag
    10741 gtgacagccc agtccttttt tatgcggtac tctcattttc aacaatgata gttctcggtc
    10801 tgataggcgg agggttttat tattatttaa aatatgtaaa gggcactcag ggtgatattc
    10861 ttgataacat cagcgaatct atagcggtta atacgggaac tcttgttgat gtaagggatg
    10921 ctcttaacaa agaccttcct gatgattata tcaacagcat aggtatctta aaacagtatc
    10981 ttaaatctga agattcagct ccgagcgctg tcggacttga cggtcaggtg aagtttaatc
    11041 ttcttatctc ctacaacaga agacttgagc cgtcggcgac acttaatgaa aaaatagatg
    11101 cagctttgaa aaaagctccg gccaatcttg atctgataaa ggcaaaagca ctctcacttt
    11161 atgaatctaa gatgtttgac gaggcacttg ttctgataca gccgtttgct gaaagcagtg
    11221 atcaggagat attttacatt ctggggctca ttgcacaggc aaaaaaagat gtgcagaagg
    11281 cggaaatgtt cttcaatcag ggttttatgc agggtggagg aaaaaactcg aagataatat
    11341 atgctcttgc cgaaatgaag tatcgaaatg gagatgctca gtctgcaatg gcattcctta
    11401 accggataat atcagacaac cccaactatt tgaaagcata tctctttaag gccggtataa
    11461 taatgaatac tgacaataag ctggaggagg ccgagaaatt tcttaaaggt gtctccacca
    11521 tgatcatttc taaagctgag gatttccaga aagccgatta ttatcagaaa cttgccacta
    11581 ttgctcataa aagaggcctg atgaaggagg cagtggggta ttatgaaaag gcagttgaaa
    11641 taaataagac agacacttct tccatagcaa caatagctga tttctatgtt cagatatcaa
    11701 attcgtcaaa agctatggaa tattatgaaa tggctctgaa gatagatccc aaatttccac
    11761 ctgcaatact cggcaaaacg gaaatattca ttcttcttgg tcagaatgat aaagtttacc
    11821 ttgaattggc taaacttgat ataaagagtg tcgtcgatgc acaattcctc ataagactcg
    11881 gaagaatcta ttacaagatc ggagacaaag gtaaatccat ggaattctat gatcttgcca
    11941 taaaatccaa cccgtcgctc gttgagccgt atctggctaa ggtggtaata cttcttgagt
    12001 ttaaaaagat aaaggagatc gatgccattg ccgctgttgt cgaaaagctc ggaaaggaaa
    12061 cctatgcatt caaccttatc aaagcggttg tgcagcacga agaggcaaat tataaaagag
    12121 ccgaagaata ttttaaaaag gcagttgaaa gaaacacagc gggagacgac agggttttct
    12181 tttattacgg gttgttcctt tttgaccagc agaactatcg ggaatcttcg aaaatgcttg
    12241 aaaaggcata taagatcgat ccgagaaact acaactacat tcagtcctat gccgacagtc
    12301 ttgaaaaaga gaagaagtgg aaggcggtta ttgatcttct tgagacaggc actttcaatg
    12361 agaaaagaat gtaccgcagt tttgttacgc ttgcgaatgc attctatcat ctaaacaaat
    12421 ttgatgaggc cttgaatttt gtgaacaggg ccatagaact taacaatcag catacgcatg
    12481 ttttccatat gaaagctaag atttattacg cgatggaaaa atataacgaa gctgaaaaag
    12541 agatagatac agcagtggta atagacatga gaaacttcga caactacatg ctttatgcga
    12601 aggttcttgc aaaaaaaggt gatttcaagg gggcgataga aaaaatagaa gcggcggaaa
    12661 agatagatcc atcagaccag cagcttatgc tgatgaaagg gatcatctat aaaaatcttg
    12721 atgattacag aagtgctcta gcgtatttca gaaaggttac caacaaaagt tttatcaaag
    12781 aagcatatct tgaaataggt gaaagttatc ttcagcttaa cagggttgat gatgctatga
    12841 agttttttaa gaaagctgaa aattcaggga acaaattagc acacaagcat cttgcaagga
    12901 tatactatga gtccggcaaa ctcgaccagg cggtgacata ttaccgtaaa gctctcaggg
    12961 cagacaagaa tgatacaacc gcaatgagac agctgggcta tatttacaaa gagaaacagg
    13021 agtggagcaa agctctgtca tatttaaaaa tgtatcagaa aaggataaat gacgccgcag
    13081 aaaagaagat gatagatgaa gaggtttatt ttcttgaaaa gaatcttcct caaggtcgtc
    13141 ccgtacatat tgccgagtct ctaaatgaac ttgacgagaa tgactccgaa gcaataaatg
    13201 aaagagcaaa ggagctttat atcgaaggtc gcgctttaag agatgaggat cctgaaactg
    13261 caagggacag attcagggaa gtaatgaaaa tcgttcctaa aacgaatgag tactataaaa
    13321 aggcattcaa agctttcaac aagcttaacg aaagcaaaga gaaataaact caaaatcctt
    13381 gatttagctg ttttagcggt atacaatcca taagtaacgg ttcgttaaag gtttactgtt
    13441 tttattcaaa aggaggagaa aatgggttgc tgctgcaatg aaaaaaaact tacaagcgct
    13501 gaacttatgc acattgaaca cacttacggt gcacacaatt accatccgct ggaagttgtc
    13561 atctgtaagg cagagggcgt ttgggtatgg gatcctgaag gtaagaaata tatggactgt
    13621 ctcagtgcct acagcgctgt aaatcaggga cacagacacc ctaagataat taaggcctta
    13681 aaagatcagg cggatattct tactcttaca agccgtgcat tttttaacga caaatggcct
    13741 cttttcgctc agaaacttgc tgaatataca gggaaggata tgatcctgcc tatgaataca
    13801 ggagcagaag ctgttgagac cgcaataaag atgacgcgcc gctggggata tgaagtcaag
    13861 ggagttcctg ctgacaaagc tgaaataatt gtgatgaaag agaatttcca cggcagaaca
    13921 acaacaatag tttccttttc caccgatcct gatgcaaaaa acaattacgg tccttttacc
    13981 cccggtttca aagttgttga atataatgat ctcaaagcta ttgaagaagc aataactcca
    14041 aatactgtgg gtgttatgct tgagcccatt cagggtgaag ctggggtttt tgttcctttt
    14101 gacggatacc tcaaggggat cagagagatc tgtgacaagc acaatgtcct ttttgtctgt
    14161 gacgaagttc aaacgggttt ctgcagaaca ggaaagaaat ttgcattcat gcacgaaggg
    14221 atagttcctg atgtaataat aatgggaaaa gctctcggtg gaggcataat gcctgtttcg
    14281 gcagttgccg caaataaaga tatcatgggt gttttcacgc ctggaacgca cggttccact
    14341 tttggtggaa atccgcttgc atgtgcagtt gcaactgcgg ctcttgatgt tcttgaagag
    14401 gaaaatcttg cacagagatc agaagagctc ggcaaatact tccgggagcg gcttgtcgca
    14461 atgaacagcc cgaaaataga gctggtcaga ggaaaaggtc ttctcaatgc agtcgttctt
    14521 aaaaaagagg caggcaaggc aaggatctat acgacagcaa tgaagaacag aggggttctt
    14581 gcaaaagaga cacacgaatg gatcatcaga tttgctcctc cgttgacagt tacaaaagaa
    14641 gagcttgact gggcgctcaa cataatcgaa gaagttctta aatagtttct cagatcggaa
    14701 ttatttctga aagatgaaat caacagttat ttcaacaagt tttggaatag aatctgcttt
    14761 tatatctgca atgatctctt cgtttccctt ctttccgccc ggtttgagaa ttacgaggtt
    14821 gaaataaacg ctcatgagat ttttgtaagt tctgactctg aagctttctc cgtcgtaagt
    14881 cattgattct cctgtgtcaa ttacagcttc aattcctttc tgctgaaaag ctcctgaaat
    14941 tccgtaaaga atggcgttgc tctcttcatc atcgacttcg ttgtagagaa taactttttt
    15001 tatcccttca aatttaaaat tctcatcttt gacagttatc cctgaatcag cccttgcccc
    15061 ttttattgtg ggatccttct ttttttcggt tgaaccgcaa ctggctgaaa acaataagaa
    15121 aacaaaaatt gttattgtg
//
LOCUS       DAOOYB010000048        14962 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_4004_length_14962_cov_15.141343, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000048 DAOOYB010000000
VERSION     DAOOYB010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 14962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14962
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4004_length_14962_cov_15.141343"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="PL056_04415"
     CDS             complement(<1..467)
                     /locus_tag="PL056_04415"
                     /inference="COORDINATES: protein motif:HMM:NF013854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HPM46288.1"
                     /translation="MKIIFATGNKGKLKEAREIFAGHEIISIKEFDPGFAPDETGDSF
                     FQNAVIKAEAAMKIVKNIAVLSDDSGLIVSVLDGAPGVYSSRFGGEEGNDLLNREKLL
                     NELSGITERSAYFSCTAVLLFPDMSAIVTNGKCSGKIGFKELGDNGFGYDPLF"
     gene            complement(464..1063)
                     /gene="trmB"
                     /locus_tag="PL056_04420"
     CDS             complement(464..1063)
                     /gene="trmB"
                     /locus_tag="PL056_04420"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HPM46289.1"
                     /translation="MITHNIELPLEEIAFKPLDFAEVFQNRNRVEIEIGFCSGFFLSE
                     YANDNPDTGLLGIEISRKFFVRGERNLKRVLKQDNVRITCFDAVPVVRELIPFNSVDA
                     IHIYFPDPWPKKKHHKFRILRFENFLLFYNRLKAGGHVYIATDHPEYGEFIRKEIAVS
                     KEFFKALPYGIEDRKIKTKWEKKQIADCWPINYFLLEKI"
     gene            complement(1070..1579)
                     /gene="hpt"
                     /locus_tag="PL056_04425"
     CDS             complement(1070..1579)
                     /gene="hpt"
                     /locus_tag="PL056_04425"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HPM46290.1"
                     /translation="MKKLICRNTIHKRVQELGTEITSFYKERPFTCLIILKGSTVFAA
                     DLIRAITSENMDIDFIRLSSYCGTHSTGKVEMLMGELEKFRGRELLVVEDIVDTGTTL
                     SFFLEKLNEIKPLTVKICSLLEKKEINKGRVKVDFLGFDIKNEFVIGYGLDMNERFRN
                     LPDIMVYEG"
     gene            complement(1576..3588)
                     /locus_tag="PL056_04430"
     CDS             complement(1576..3588)
                     /locus_tag="PL056_04430"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPM46291.1"
                     /translation="MRKILIVDSDEQITAVLGLKLRKENFEVKTIASALKAVDVVKEF
                     KPDLIISEMVLAEVSGVDFLKRVKMNPDTMKTPFIFLSNSRNVEDKILAHEMGAEAFF
                     MKPIFIKVLINRIKDFFDEKNFKEMISSKEAKSFSGELTSISIIDILNIISENMSTGE
                     VLIASSTSEKATIFFVNGSVVRVETGDGTGRNGVDELFKILSWLDGTFTINYKDIMVV
                     RNINIPHDRLVMRAVNWFEDYSGELSEMPPTDSLVFVDFGKLMNNINKFPDGIGEILK
                     NIGSEGSKISSIIDNTPFDKRQTAKYLKKMMEINVLSLENRDTEFNVPKEPVWFTGGN
                     EKRNTAPQSEYLNDTSISTFKDLDIDEVIDEKDRAEEGKTLVPPPSFAKKDTLDPALG
                     IDIDFNDSREGGNERKADHSININIDILEERPAEDKTEIIRTVSDDDEIKVKSRKETE
                     ELLSMHKNPEKRPAGRKALLFFIFLFLSAGVIFAAYVQYPEKFSSIISMFPVEEIESV
                     PENTVVETEVENEVSQQQPAEIVLTEEQKELAQKSVAELISMSTERFDLEKYGEAIDL
                     NRVALYKFKVEKTESGEEYEKVVTNMAIFLYTAEKYEEALEFAEESLLLRNDEKSIEL
                     KAAILEELKRPAAAASLLRTKLNDEKFSHKKEEWMMEINRLENIQR"
     gene            complement(3566..4102)
                     /locus_tag="PL056_04435"
     CDS             complement(3566..4102)
                     /locus_tag="PL056_04435"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM46292.1"
                     /translation="MKEPQPALELNFEIRSDLNLIPGVVKKIIESSSGMFDDLYMLEV
                     AIYEAVYNAIEHGNLEITRSRKEKMIEEGTYDDFLKGRASKENCVSKKVEIKLICCKE
                     NLKIEIKDEGKGFNWKEEMTNAKIFDGKDLNQFNGYGLRIILSVFDEVFYNESGNHLT
                     MIKFRKNMEERIEKNTDC"
     gene            complement(4120..5196)
                     /gene="cheB"
                     /locus_tag="PL056_04440"
     CDS             complement(4120..5196)
                     /gene="cheB"
                     /locus_tag="PL056_04440"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HPM46293.1"
                     /translation="MTGKKRVLIVDDSRTAREVLAEIISGFEDFEVAGKASDPFEAHD
                     FMKKEMPDIVTLDIEMPKMDGLTFLDKIMRGAPLPVIMISSLTTENAAFTLKALELGA
                     VDYVLKPGGDESLKDVAQIIHEKLVAASMVPKSWLNLHKYRKKDESLSAFSRRTIKLV
                     PIDDSPKMNPDVSLIVMGASTGGTMVIEKVLSKLDHRKMPPIAVVQHIPPFFSKSFAD
                     RLNKLFPFNVYEAENGKLMLPGDVIIAQGGKHLLVQKAPNGFAGLVKEGPKVNRHQPS
                     VDVLFKSAAIVYRSNVIGVLMTGMGSDGAAGMVELKKNGAVTIAQDRYECAVAGMPRS
                     AIDLGVVDKELDSERIADYLNSVF"
     gene            complement(5193..5813)
                     /locus_tag="PL056_04445"
     CDS             complement(5193..5813)
                     /locus_tag="PL056_04445"
                     /inference="COORDINATES: protein motif:HMM:NF015908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemoreceptor glutamine deamidase CheD"
                     /protein_id="HPM46294.1"
                     /translation="MYKSFNKKLQKDIIVIQPGEYFVVDRSSDGIATVLGSCVAVCLI
                     DAEKCIGGMNHFMLPGDFRNDEVFASKSSRYGMFAMESLINEMMKRGAGREKLVAKIF
                     GGGHVLNFRKSDGNIPQANINFVKSYLEFEEIPVVASDLGGDVGRKVIFLPSEGGRVL
                     VKKLMKSPENAIDVENKYKAKVLNKKQDDVSGGVTIFLKDHQEEIK"
     gene            complement(5822..6679)
                     /locus_tag="PL056_04450"
     CDS             complement(5822..6679)
                     /locus_tag="PL056_04450"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HPM46295.1"
                     /translation="MNTSKFEIKKISLSDSEFDKLRELVYKISGISLANTKKELVISR
                     FSKRLKELKLSSFGEYHDLLVSPGGFSEVQNFINSITTNKTDFFRESHHFDFIVSTFI
                     PQVVASARPVVRVWSAACSTGEEPYTIAMVMSKYLVEPYGIPVKILATDIDTNVLKAA
                     ARGVYDSHAIGQVPEKYLKKYFLRGKGDSFGLFKVKDDIRSMVTFKQLNFIAPEYPIT
                     ATFDIIFCRNVIIYFSPETKKQVVGKLFRYLSEGGYIMMGHSETLFNMIEGLVYLKNT
                     VYQKRIMDK"
     gene            complement(6669..8009)
                     /locus_tag="PL056_04455"
     CDS             complement(6669..8009)
                     /locus_tag="PL056_04455"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HPM46296.1"
                     /translation="MSDFFNLISEFINESDSQLAEIRKCFPAFIKSLKNCEIPSYEKN
                     SRLLRAVHSISGGSSFSGLTEIEKISSKLEKMIYLAISGKIEPCEILAAAVEKTLAVL
                     EEGLSSNGKIKKKEWAKIEKDVERIIFSSSGSHVPENFHSEIIISFEGETATFSINDP
                     DVPEKLRSQNLYLLKFDAETDLREDELSLFQMITILAGEGDILDSMIEIDQVGEESVP
                     IKILYMSTLALNKLKKLLNFLPSKSIKKIDAKSIEIAFDIKTEDLPESHAVEKSKPEP
                     VENIIQIEEKIKNPVSSNDDDDDQEEAASNKYVSFKIGTEHYAVPIEFVYDMKEMLPC
                     SRIPNQPDTYLGVANLRGNVIPVIDLRRVFGIKNVKYDEFTVFLMVKIENKIKGCVVD
                     SIDDVVFLEPENTQITPALSRKIKTDFVKFIAKDPKTERFLIVIDVEKMLENEH"
     gene            complement(8018..8524)
                     /locus_tag="PL056_04460"
     CDS             complement(8018..8524)
                     /locus_tag="PL056_04460"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HPM46297.1"
                     /translation="MNRSGFAENLKKENEAKEEKFLQFVSFMVGEEEYATDILKVYEI
                     TSYKKLTKVPSMPPFVKGVLSIKDIIVPVIDLREKFSIQDPANTKFTIIMIVDVSGRV
                     TGVVVDRMKDIIKVKASDIQPPPRFSTGVKTSFIRGMIKKGKDDFTIVLDMAKILSDY
                     ELNIMDTL"
     gene            complement(8521..10626)
                     /locus_tag="PL056_04465"
     CDS             complement(8521..10626)
                     /locus_tag="PL056_04465"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HPM46298.1"
                     /translation="MNKKNSLYTIFLEEAAELVSGLENDLLVLESNMSDRQLVDQIFR
                     AVHTLKGSSGMVEYSLFTSLAHEFENILSRVRSGDLKITGNLITVFLNSTDVLKQLVS
                     VDPKISERELGPEVRVAVENLKKFKGMQPEFDPVEQKTAGMAGENFSEKHIQITLKFK
                     PDIFVSGTDPLMLLKELSQMGEFVRIKCNPGAVPVLDSFKYESLYLSWELILKTDFPV
                     SSVENVFVFVKENNTIVVEDISSKFVEGVDLRYADKMLGEILEEEGYVTENDLKKIAE
                     TQKRVGEILVENKNVDTSVVNRLLKSQQKSRDFQVSQTIRVDAYKLDRLVNLVGELVI
                     GIARVNQIVTENHYKNRELNDASEHLERISRYIQEQVMKTRMIPIEGTFRNFQRVVRD
                     MAGELSKRIKLFLAGSETELDKTLIEQIDAPLKHLIRNSIDHGIGSPEERLSTGKPVE
                     GSIWMRAYQQEGKIIIEVEDDGKGIDKKEVLKKATERGLYKGGDLSDKEIYNFLFTPG
                     FSTAKSVTEFSGRGVGLDVVRKNIENLRGSVEVFSEKGKGTLFRIKLPLTLAIIEGMQ
                     VSIGKEVITVPLFSIIEAVRPESNIVKTIEGKGELIEFRGGFIPLIRLYEVFDFPTDV
                     TDPLNGFILILDGHNRKIALMVDDIIGQQQVVIKSLEKNHHQIPGISGATVLGDGRIS
                     LILDVYGLEKLAFGEKEKK"
     gene            complement(10631..11008)
                     /locus_tag="PL056_04470"
     CDS             complement(10631..11008)
                     /locus_tag="PL056_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPM46299.1"
                     /translation="MNKTILIVDDSATLRASVNFVLTDAGYSVIQAKNGEEGLAKLLE
                     NKRAGEKVDMIISDINMPVMDGITFIKEVKKGDFRFIPILILTTESENSKKMEGKAAG
                     ASGWLVKPFKPEQLVWVVKKFVR"
     gene            complement(11005..11292)
                     /locus_tag="PL056_04475"
     CDS             complement(11005..11292)
                     /locus_tag="PL056_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46300.1"
                     /translation="MANFKIDGTIIYISGDISISEGESLYNFLRGMPIFKEEITVDMK
                     RVESWDTSSFQTFISWIKSTEMSIKWKNIPEEMSKDLKITGLSTLFKGVQK"
     gene            complement(11294..11602)
                     /locus_tag="PL056_04480"
     CDS             complement(11294..11602)
                     /locus_tag="PL056_04480"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPM46301.1"
                     /translation="MINFNIDHSEQVSTLTVKGRIDNEGIQGFQDALNALLEKESKTV
                     ILNFNDLEFINSSGIGKLLIFYKKSKNFGRETSINGISDDVFTLFKAIRLDKLIDIKR
                     "
     gene            complement(11631..12338)
                     /locus_tag="PL056_04485"
     CDS             complement(11631..12338)
                     /locus_tag="PL056_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46302.1"
                     /translation="MEYLKNYIDILKDMLSTVFDHTEKTCHECKLMINSLDSSVASID
                     ELRESVAELTVLFQFGDIIFQSFESFDVIFDELSRLESPDGKISYIQAEKFSKLIGAV
                     SANTFTMTGRTLVNLKENLISARDILCKIENSTKNSFGDEKMSSLGEASEKCRKFVEN
                     NILQMESSVRRAVIRTEGLGLFSVDTIFSSKHSLNDKILSDKLHEKIRVREHIEILNG
                     LFPTGKSYSETGSLELF"
     gene            12479..13012
                     /locus_tag="PL056_04490"
     CDS             12479..13012
                     /locus_tag="PL056_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46303.1"
                     /translation="MVRKILFLLTISLILGCLLTFCSKKEPEKIDTSISQEDIDLLIK
                     HKKNIDRITGKYDAEMQKVKQHEKPAIIQKGKDEIDNYLKSNNLSPVVFMRKSKKILK
                     GYLAFLETSEESLQKKTEMLNKEGLKEEEIKQKAALYKKAGENAFKELTSELSDYEIQ
                     LIKSNLKNLSVVVKQGR"
     gene            complement(13009..13812)
                     /locus_tag="PL056_04495"
     CDS             complement(13009..13812)
                     /locus_tag="PL056_04495"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HPM46304.1"
                     /translation="MTKFEVLVPLAEGGKEVVVREEGPNWFCALESALSGLGMADSMK
                     NVICDVKEDGAIHVTDTASGRKFLVRELDLTKNIQKIKEEPKKEEPKVTRKDEMLADL
                     FTDVMDAWDLPEKDIPGFFLDLALKYIPSESGSFAKSDLSSTDMEFVACRGPKGDDVC
                     GIKIKVGQGLVGFSAKHSCFIAVGDVHKDPRFFKEISEKIGYETTSIACAPVKSLETT
                     ITFGVLELINKKNSYRYNEDDLEFLRFIAEKMAELFHSRWTESNNNLED"
     gene            complement(13893..14468)
                     /locus_tag="PL056_04500"
     CDS             complement(13893..14468)
                     /locus_tag="PL056_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46305.1"
                     /translation="MKFHQRISQLAEKIEHLEHSFNFYFTAQEKKPPLDKLNRLKKEI
                     DELMKVSQDLKNSAERFLAMQIVNRFTSYRIKWEKGVKDIEEGRAKPGLHFFGGLGIG
                     RSPMDDIKKSADELAKKDANAFRMSSVIDEAAEKYIKMSKKYTGKTFDKEAVTSMLEK
                     KIEEVRKKFGDRFKFSVVYEEGKVRIKPEKE"
     gene            complement(14481..>14962)
                     /locus_tag="PL056_04505"
     CDS             complement(14481..>14962)
                     /locus_tag="PL056_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46306.1"
                     /translation="NLKERVDKIKEEMKRDSIEIPIDPELIETAKLYKDKIEVTNVRN
                     GEVEFGNGLSQYMMDATVKNNSDKHLSYVGVLVKFMDDANQKVLFEKTFFLIYTRQIE
                     EIYPLAPGEEKNIIIPGYDAGDIEGNWTGKLQWEVNAVKVATPAEMIKIEDLNLPKK"
BASE COUNT         4065 a         3338 c         2539 g         5020 t
ORIGIN      
        1 atgaatagcg gatcatatcc gaaaccgttg tcgccaagct ctttaaatcc aatttttcct
       61 gaacattttc cgttagttac aattgccgac atatcgggga aaagcagcac cgcagtacac
      121 gaaaaatatg cacttctttc agtgattccg cttaactcgt ttaaaagctt ttcccggttt
      181 aataagtcat tcccctcctc tcctccgaac cttgaagaat atactcccgg agccccgtca
      241 agcacagaaa caataagccc cgaatcatca cttaacacag caatattttt aactattttc
      301 attgccgcct cagctttaat caccgcattc tggaaaaacg aatccccagt ctcatcagga
      361 gcaaaacccg gatcaaattc cttaatcgaa atgatctcat gacccgcaaa aatctctctt
      421 gcttccttta attttccctt atttccggtc gcaaaaatta ttttcatatt ttctccagca
      481 gaaaataatt gatgggccag cagtcagcga tctgtttttt ctcccatttg gttttgattt
      541 tgcggtcttc aatgccgtaa gggagggctt tgaaaaattc ttttgagaca gcaatttctt
      601 ttctgatgaa ttctccatat tcggggtgat cggtcgcgat ataaacgtga ccgccggctt
      661 taagccggtt atagaaaagc aaaaaatttt caaagcgtaa gattctgaat ttatgatgtt
      721 ttttctttgg ccatggatca ggaaaataga tgtgtattgc atcaactgag ttaaaaggga
      781 tcaactctct taccacggga actgcatcaa aacaggttat ccttacattg tcctgtttaa
      841 gaactctttt aaggttccgc tctcctctta caaaaaattt ccggcttatt tctatgccaa
      901 gaagtccagt gtcggggtta tcgtttgcat attcggaaag aaaaaatccg ctgcagaaac
      961 cgatttcaat ctcaaccctg ttcctgtttt gaaaaacttc agcaaaatca agcggtttaa
     1021 aggcaatttc ttctaatggg agctctatgt tgtgagttat cattgaaaat cagccctcgt
     1081 aaaccattat gtccggaaga tttctgaacc tttcgttcat atccagacca taaccgatca
     1141 caaattcgtt tttgatgtca aacccgagaa aatcaacttt tacccgtcct ttgtttatct
     1201 cctttttttc aagaagtgaa catattttaa cagttaatgg cttaatttcg ttaagttttt
     1261 caagaaagaa agaaagcgtt gtccctgtat caacgatgtc ttcaacaaca agaagttccc
     1321 tccctctgaa tttctccagc tcccccatca gcatctcgac tttgcctgtt gaatgagtac
     1381 cgcaatagct tgaaagccgg ataaaatcaa tatccatatt ttcagaagtt atagccctta
     1441 tcagatcagc tgcaaaaact gtgctccctt taagaattat aagacatgta aaagggcgtt
     1501 ctttgtaaaa tgaagttatt tcagttccaa gttcctgaac tcttttgtga atggtgtttc
     1561 tgcaaataag ttttttcatc tctgtatgtt ctccaggcgg ttaatttcca tcatccattc
     1621 ttcttttttg tgactgaatt tttcatcgtt taacttggtt cttaaaagtg atgccgctgc
     1681 tgccggtctt ttaagttcct caagtatcgc ggccttaagt tcaatcgatt tttcatcatt
     1741 tctaaggaga agtgattctt cagcaaattc aagagcttct tcgtatttct cagccgtata
     1801 gagaaatatt gccatattgg tcacaacctt ttcatactct tccccgctct cagttttttc
     1861 aactttgaac ttgtaaagag cgaccctgtt taaatctatt gcctcgccat atttttccaa
     1921 atcaaaccgc tcagttgaca tgcttatcag ttctgcaact gacttctgtg ccagttcttt
     1981 ctgttcttcg gttagaacga tctcagccgg ttgctgctgt gaaacttcat tttcaacctc
     2041 agtttctacc acggtattct caggaacaga ttcaatttcc tcaacaggga acattgagat
     2101 aatacttgag aatttttcag gatattgaac ataagccgca aatattacac cggcagacag
     2161 aaacagaaat atgaaaaaaa gaagagcttt ccttcctgct ggtctttttt cggggttttt
     2221 gtgcatcgaa agaagttctt ccgtctcttt gcggctttta acttttattt catcatcatc
     2281 agaaacagtt ctgattattt cagttttatc ctccgccggc ctttcttcaa gaatatcaat
     2341 gtttatattt atggaatggt ccgctttcct ttcattgccg ccttccctgc tgtcattaaa
     2401 gtcgatgtct attcccaaag caggatcgag agtatctttt tttgcaaaag atggtggcgg
     2461 aaccagcgtt ttcccctctt ctgcacgatc tttttcatca atgacttcat caatatcaag
     2521 atctttgaat gttgaaatgc tcgtgtcatt taaatattca ctctgcgggg cggtatttct
     2581 tttttcattt ccgcctgtaa accagaccgg ttctttcggt acattgaatt ctgtatctct
     2641 gttttcaagc gagaggacat ttatctccat cattttcttc agatatttag ctgtctgtct
     2701 tttgtcaaaa ggagtattgt ctatgatgga actgatcttg ctcccttcgc tcccgatatt
     2761 tttaaggatt tcgccaatcc cgtcagggaa cttattgata ttgttcatta attttccaaa
     2821 atccacaaaa acaagagagt ccgtgggagg catttcacta agttccccag aatagtcttc
     2881 aaaccagttg acagctctca ttacaagccg gtcgtgcgga atattaatgt ttctcacgac
     2941 cataatgtct ttgtaattaa tggtgaaagt tccgtccagc cacgaaagta tcttgaaaag
     3001 ttcatcaacg ccgttccttc cggtgccgtc acctgtttca actcttacaa cagatccatt
     3061 gacaaaaaat atggtggctt tttcagaagt tgaagatgcg atcagaacct ctcctgtgga
     3121 catgttttct gaaataatgt taagtatgtc aattatggaa atgctggtga gttcaccgct
     3181 gaagcttttg gcctcttttg acgaaatcat ctctttgaag ttcttttcat cgaaaaaatc
     3241 ttttattctg tttatgagaa ctttaatgaa aatcggtttc atgaaaaaag cttccgctcc
     3301 catttcgtgg gcaaggatct tgtcttcaac atttcttgag tttgaaagaa aaataaacgg
     3361 tgttttcatc gtgtcagggt tcattttgac ccttttgaga aaatcgactc cgcttacttc
     3421 agccagaacc atttctgaaa taatcaggtc gggcttgaac tctttcacaa catcaacggc
     3481 ttttaaagcg gaagctattg ttttcacttc aaaattctct ttgcggagtt tcaaacccag
     3541 aactgcggtg atctgttcgt ctgaatcaac aatcagtatt tttctcaatt ctctcctcca
     3601 tgttttttct gaacttgatc atcgtgaggt ggtttccaga ttcattataa aaaacttcat
     3661 caaatacaga aaggatgatc ctcagtccgt aaccgttgaa ctgatttaag tccttgccgt
     3721 caaatatttt tgcgttcgtc atttcctctt tccagttaaa tccctttcct tcgtctttta
     3781 tctcgatctt aagattttct ttgcagcata ttaattttat ttcgactttt ttgctgacac
     3841 aattttcttt actggccctt ccttttaaga aatcatcata agtgccttct tctatcattt
     3901 tttctttccg gcttctcgtt atctcaagat tgccgtgttc tatggcattg tataccgctt
     3961 catagatcgc aacttcaagc atataaagat catcaaacat ccccgaagag gattctatga
     4021 tcttttttac gacaccggga atgaggttaa ggtcactcct gatctcaaaa ttcaattcca
     4081 atgcaggttg cggttctttc atttttcccc gtgaataacc taaaaaactg aattcaagta
     4141 atccgctatt ctttctgaat cgagttcctt gtccacgaca ccgagatcta ttgccgatct
     4201 cggcattcct gcaacagcac attcatatct gtcctgagca atcgttactg ctccgttctt
     4261 tttcagctcc accattcccg cggctccgtc tgatcccatt cctgtcataa ggacaccgat
     4321 gacattcgac ctgtaaacaa ttgccgccga tttgaacaga acatccactg agggctgatg
     4381 cctgttcact ttcggacctt ctttcacaag accggcaaac ccattcggcg ccttctgcac
     4441 aagaagatgt tttccgccct gagctattat gacatcaccc ggaagcataa gttttccgtt
     4501 ttcagcttca tagacattga aagggaaaag cttattcaag cggtctgcaa aagatttaga
     4561 aaagaacggg ggaatatgct gaaccactgc tatgggaggc atttttctgt ggtcaagctt
     4621 tgaaagcact ttctcgatta ccattgtccc gcctgtagaa gcgcccatta ctatcagcga
     4681 gacatccgga ttcatctttg gagaatcgtc tatgggaaca agctttattg tccttcttga
     4741 aaaagcactc agtgattcgt ctttttttct gtatttgtga agattaagcc agcttttggg
     4801 aaccattgat gccgcaacaa gtttttcgtg aattatctgg gcaacatctt tcaaagattc
     4861 atctccgccc ggctttaaaa cataatcaac ggcaccaagt tcgagggctt tcagcgtaaa
     4921 agcggcattt tctgtcgtaa gcgaacttat cattattaca ggaagaggag cgcctctcat
     4981 aattttatcg aggaaagtga gcccgtccat tttgggcatt tctatatcca gagtaacaat
     5041 gtcaggcatc tcctttttca tgaaatcgtg tgcctcaaaa ggatccgatg cctttcctgc
     5101 aacttcaaaa tcttcaaaac cgctaatgat ctctgcaaga acttctcttg ccgttcttga
     5161 atcatcaact ataagaaccc gtttttttcc tgtcatttga tctcttcctg gtgatcttta
     5221 agaaatattg tcacgccacc actgacatca tcctgttttt tattaagcac ttttgcttta
     5281 tatttatttt caacatcgat agcgttttca ggacttttca tcaatttttt taccagaacg
     5341 cgtcctccct cgctcggcag aaatatcact tttctcccga catctcctcc aagatcgctt
     5401 gcaacaacgg ggatctcttc aaattcaaga taacttttca caaaattgat gtttgcctga
     5461 ggaatattgc catctgattt tctgaagttc agaacatgac ctcctccgaa tatctttgca
     5521 acaagttttt ccctcccggc acctcttttc atcatttcat ttataagact ttccattgca
     5581 aacataccgt aacggctgct ttttgaagca aaaacctcgt cgtttctgaa atctccggga
     5641 agcatgaagt gattcattcc tccgatacat ttttcagcat cgatcagaca tactgcgaca
     5701 cacgacccaa gaacagtggc aatcccgtca gaagatctgt ccacaacaaa atattctccc
     5761 ggctggatca caatgatgtc tttctgaagt tttttgttaa agcttttata catttctctt
     5821 cctacttgtc catgatcctt ttctgataaa cggtattctt taaataaacc agcccttcta
     5881 tcatattgaa aagtgtttca gaatgaccca tcattatata cccgccttcg cttaaatatc
     5941 tgaaaagctt tccaacaacc tgcttttttg tctccgggct gaaatagatt atgacatttc
     6001 tgcagaaaat aatgtcaaaa gttgcagtta tcggatattc cggagctatg aagttaagct
     6061 gtttgaatgt gaccatgctt ctgatatcat cttttacttt gaagagaccg aagctgtccc
     6121 cttttcctct tagaaaatat tttttgagat acttttcagg aacctgtcct attgcgtgtg
     6181 aatcataaac accccttgcc gccgctttaa gaacattcgt gtcaatatcg gttgcaagga
     6241 tcttcaccgg tattccgtac ggctcaacca ggtattttga cattaccata gcgatggtat
     6301 agggctcttc acccgttgag catgcggcag accacaccct gacgacgggg cgcgccgatg
     6361 cgacaacctg cgggatgaat gttgaaacaa taaaatcaaa atgatgtgat tctctgaaaa
     6421 aatcggtttt atttgtagtt atggaattga taaagttctg tacctcggaa aagccgccgg
     6481 gagagacaag aagatcatga tattctccga agctgctgag tttcaactct ttgagtcttt
     6541 ttgagaatct tgatataacg agttcctttt tcgtgtttgc aagacttatg cctgaaattt
     6601 tataaaccag ctccctcagt ttatcaaatt cgctgtcgct taaagaaatt ttcttaattt
     6661 caaatttgct agtgttcatt ttcgagcatt ttctctacat caattactat taaaaatctt
     6721 tctgttttcg ggtctttggc aataaattta acaaaatcgg tttttatttt acggctcaat
     6781 gcgggagtta tctgagtgtt ttccggctca agaaaaacga catcgtctat cgagtcaaca
     6841 acacatcctt tgatcttgtt ctctattttt accatcagaa aaaccgtaaa ttcatcatat
     6901 tttacatttt tgatgccgaa aactcttctg aggtctatta ccggaatgac attgcctctc
     6961 aggtttgcaa ctcccagata tgtatcgggc tgattcggta ttctcgaaca ggggagcatc
     7021 tctttcatgt catatacaaa ttctatcgga acagcatagt gttctgttcc gatcttgaaa
     7081 gaaacatatt tgttgcttgc tgcttcctcc tggtcatcat catcgtcgtt agatgaaacc
     7141 ggatttttaa ttttctcttc gatttgaata atgttttcaa cgggttccgg cttgcttttt
     7201 tcaacagcat gggactccgg aagatcttct gtttttatgt caaaagcaat ttctattgat
     7261 tttgcatcga tcttttttat agattttgac gggagaaaat ttaaaagttt tttcaactta
     7321 ttcaaagcaa gagtgctcat atagagtatt ttgataggaa ccgactcttc tccaacctga
     7381 tctatctcta tcattgaatc aagtatgtca ccttctcctg caaggatggt tatcatctga
     7441 aaaagagaaa gctcatcttc gcgaagatcg gtttcggcat caaattttaa aagataaagg
     7501 ttctgtgatc tgagtttttc cggcacatca ggatcgttaa tcgaaaaagt tgccgtctca
     7561 ccttcaaaac ttattataat ttcgctgtga aaattttcag gaacatggct gccggaagaa
     7621 gagaaaatta tcctttcgac atctttttct attttggccc attccttttt ttttattttt
     7681 ccattactgc tgagcccttc ctcaagaact gccagtgttt tttcaacggc ggcagcaaga
     7741 atttcacagg gttctatctt tcccgaaatt gcaagatata tcattttttc gagctttgag
     7801 gatatctttt ctatttctgt aagaccggaa aaggaactcc ctccgcttat tgagtgaact
     7861 gctcttaaaa gacgggagtt tttttcataa gaggggattt cacaattttt cagactttta
     7921 ataaaagccg gaaaacattt cctgatttca gcaagctgag aatcagattc attgatgaac
     7981 tccgaaataa gattaaaaaa atcgctcatt gtcaccttta aagagtgtcc attatgttaa
     8041 gttcatagtc tgaaaggatc tttgccatat ccagcactat tgtaaaatca tctttccctt
     8101 tttttatcat tccccttatg aaacttgtct tcacgccggt tgaaaaccgc ggtggcggct
     8161 gtatgtcaga tgctttcact tttataatat ccttcattct gtcaacgacc acacctgtaa
     8221 ctctgccgct cacatcaacg atcatgataa ttgtaaattt tgtgtttgcc ggatcctgta
     8281 tgctgaactt ctctctgagg tcaataactg gaacaataat gtcctttata cttaaaacac
     8341 ctttcacaaa agggggcatg gaaggcactt ttgtaagctt tttataactt gttatctcat
     8401 atacttttaa aatgtcggtc gcatactcct cttcgcccac catgaaagag acgaactgaa
     8461 gaaatttttc ctcttttgcc tcattttctt tttttaaatt ctccgcaaaa ccggatctgt
     8521 tcattttttc tccttttccc cgaaagccag tttttcaagc ccgtaaacat caagaataag
     8581 cgatatcctg ccatctccaa gcactgtagc tccgcttata cccgggatct gatggtgatt
     8641 tttctcaaga ctttttatga caacctgctg ctgaccgatg atatcatcga ccattaaggc
     8701 aatttttctg ttgtgtccgt caagtatgag aatgaatccg ttaagcggat ctgtcacatc
     8761 ggttggaaaa tcgaaaacct catacagtct tataagcggg atgaatccgc ccctgaactc
     8821 aatcagctcc ccttttcctt ctatcgtttt tacaatgttg ctttccggcc ttaccgcttc
     8881 aattatcgag aaaagcggaa cagtaatgac ctcttttccg atagatacct gcattccttc
     8941 tattatcgcc agagtgagcg gaagctttat tctgaaaaga gttcccttcc ctttttctga
     9001 aaaaacctca acactcccgc gcagattttc gatgttcttt ctcacaacat caagcccgac
     9061 acctctgccg ctgaattcgg tgacactttt cgccgttgaa aatccaggag tgaagaggaa
     9121 attgtatatt tccttgtctg aaaggtcccc tcctttataa agccctcttt cggtcgcttt
     9181 tttgagcact tcctttttgt caatcccttt tccgtcatct tccacttcaa tgattatctt
     9241 cccttcctgc tggtaagctc tcatccagat cgaaccctcg acgggcttgc ctgtgctcaa
     9301 tctctcttcc ggagagccga tcccgtgatc gatcgaattt cttataagat gtttgagcgg
     9361 agcatctatc tgttctataa gcgttttgtc aagctccgtc tccgaaccgg caagaaaaag
     9421 ctttattctt ttgctgagct ccccggccat atctcttaca actctctgaa agtttctgaa
     9481 tgtcccttct atcggtatca ttctggtctt cataacctgt tcctgaatat accggcttat
     9541 tctttcaaga tgttccgaag catcattaag ctctctgttt ttataatgat tttcggtaac
     9601 tatctgattt actctcgcaa ttccaataac aagttccccg acaaggttta caagccggtc
     9661 aagcttgtat gcatcaactc ttattgtctg acttacctga aagtccctgc ttttctgctg
     9721 acttttaagc aacctgttga caacactggt atcgacattt ttattttcta ccagtatctc
     9781 accgaccctt ttctgggttt cagcaatttt cttaagatcg ttttcagtta catacccttc
     9841 ctcttccagt atttccccaa gcattttgtc ggcatatctc agatcaacgc cttctacaaa
     9901 cttagaactt atatcctcca caacgatcgt gttgttttcc ttaacaaaaa caaatacatt
     9961 ttcaacagat gaaacaggaa aatcggtctt taaaataagc tcccagctca aatacaatga
    10021 ttcatattta aagctgtcca gaaccggcac agcacccggg ttgcatttta tccttacgaa
    10081 ctcacccatc tgggaaagct ccttcagaag catcagagga tccgttcctg aaacaaatat
    10141 atccggcttg aacttgagcg ttatctgaat gtgtttttcg gaaaagttct cacctgccat
    10201 tcctgcggtt ttctgttcaa ccgggtcgaa ttccggctgc atccctttga atttcttcaa
    10261 gttttctaca gcaaccctta cctcaggtcc caactctctt tctgagattt tagggtcgac
    10321 cgacaccagc tgctttaaca catccgttga attaaggaac accgttataa gatttcctgt
    10381 tattttaaga tctcccgacc taacgcggga aaggatgttt tcaaattcat gggcaagtga
    10441 tgtaaaaaga gaatactcaa ccattccgga actgcctttg agagtgtgaa ccgccctgaa
    10501 gatctgatca acaagctggc ggtcggacat gttcgactca agaacaagaa gatcgttctc
    10561 aagtcccgaa accagctcag cggcttcttc cagaaagatt gtgtaaagcg aatttttctt
    10621 gttcataaag ttatcttaca aattttttta caacccagac aagctgctcc ggcttaaacg
    10681 gtttcacaag ccagcccgat gcgcctgcgg ctttgccttc catttttttc gagttttcag
    10741 attcagttgt caatatgagg atgggaatga acctgaagtc gcctttttta acttccttta
    10801 tgaaagttat cccatccatt accggcatat ttatgtctga aattatcata tccacttttt
    10861 cgccagccct tttattttca agaagttttg caagaccctc ttccccgttc tttgcctgta
    10921 tgacactgta tcctgcatca gtgagaacaa aattcaccga agcccgcaga gtggcagaat
    10981 catcaactat caatatggtt ttattcattt ttgaactccc ttaaaaagag ttgaaagccc
    11041 tgttatttta agatcttttg acatttcctc aggaatgttt ttccacttta tggacatttc
    11101 tgtggacttt atccacgaaa taaaagtctg aaaagaagat gtgtcccacg actcaaccct
    11161 tttcatatca acagtgatct cttctttaaa aatgggcatt cctcttaaaa aattatacag
    11221 gctttcccct tcactgatcg aaatgtcacc ggatatgtaa ataattgttc catctatctt
    11281 gaaatttgcc attttatctc tttatatcga taagtttgtc cagcctgatg gctttgaaaa
    11341 gtgtaaatac atcatcgctt atgccgttga tggatgtttc tcttccaaaa ttttttgatt
    11401 ttttataaaa gatcaggagt ttgccgatac ctgagctgtt aataaactca agatcgttaa
    11461 aatttaatat cactgttttt gattcttttt ccaaaagagc attcagtgca tcctgaaaac
    11521 cctggatgcc ttcattgtca attcttcctt taaccgttaa agtggaaacc tgttcgctgt
    11581 gatcaatgtt aaagttaatc atctcattcc tcctgaaaaa tattatgtca tcaaaaaagt
    11641 tcaagacttc ctgtttcaga gtagctttta ccagtaggaa acagtccgtt aagtatttca
    11701 atatgctctc ttactcttat tttctcgtgc agtttgtccg acaggatctt gtcgttcaat
    11761 gaatgttttg aagaaaaaat tgtatcaaca gaaaaaagtc ccagcccttc agttcttata
    11821 actgcacggc gcacagaact ttccatttgg agaatgttgt tttcaacaaa tttacggcat
    11881 ttttcagatg cctcacccag actggacatt ttttcatcgc cgaaactgtt ttttgtgctg
    11941 ttttcaattt tacacaggat gtctctggca gatatcagat tttctttcag gtttaccagc
    12001 gtccttcccg tcatagtaaa tgtatttgct gaaaccgctc ctatcagttt tgagaatttt
    12061 tctgcctgaa tataactgat cttgccgtca gggctttcaa gtcgtgagag ctcatcaaaa
    12121 ataacatcaa aactttcaaa gctctgaaat atgatatctc caaactgaaa aagaacggtg
    12181 agctcggcta cagactctct tagttcatca atagaggcga cagaactgtc cagggagttt
    12241 atcattaatt tgcactcatg acaggttttt tctgtgtgat caaaaactgt tgaaagcata
    12301 tctttaagaa tgtcgatata attttttaaa tattccaaag agtcttcacc tcataaatct
    12361 cacgcataat ataaccttaa attttttagc ggtcaatttt agatacatac catttgaata
    12421 aatttgtatt tgtcagatat ttactgtaac atcctctgat gttttttgga acgggaaaat
    12481 ggtgcgaaaa attctctttt tactgacaat atccttaatt ttgggttgtt tgctgacatt
    12541 ctgttcaaaa aaagagcctg aaaaaataga tacttccata tctcaagaag atatcgatct
    12601 tcttataaaa cacaaaaaga acatcgaccg tataacagga aaatacgatg ccgaaatgca
    12661 gaaagtgaaa cagcacgaaa aaccggcaat tattcaaaag ggaaaagacg aaatagataa
    12721 ttatttgaaa tccaacaatc tctcccctgt tgtttttatg agaaaatcaa aaaaaatatt
    12781 aaagggttat cttgcttttt tggaaacaag cgaagaatca cttcaaaaaa agacggagat
    12841 gttgaataaa gaaggattga aggaagaaga aataaaacag aaagccgctt tatataaaaa
    12901 ggctggcgaa aatgctttta aggaattaac atccgaactt tcagattatg agatacagct
    12961 tataaaatca aaccttaaga acctttctgt tgttgttaaa caaggccgct agtcttcaag
    13021 attgttgttg gattcagtcc accttgaatg aaaaagttcc gccatttttt ctgctatgaa
    13081 ccttaaaaat tcaagatcat cttcgttata acggtagctg ttctttttat ttataagttc
    13141 aagaacgcca aaagtgatcg tggtttcaag agacttcaca ggtgcacagg caatagaagt
    13201 agtctcataa cctatctttt cgcttatctc tttaaaaaaa cgggggtctt tatgaacatc
    13261 gcctacagca ataaaacagc tgtgctttgc agaaaaacct accagaccct gaccgacctt
    13321 tatctttatt ccgcagacat catcaccttt cgggcccctg catgcaacaa actccatatc
    13381 cgttgaactc agatccgatt ttgcaaaaga gccgctctca gaaggaatat atttaagcgc
    13441 cagatcaaga aaaaatcccg ggatatcctt ttccggcaaa tcccaggcat ccattacatc
    13501 tgtgaaaaga tcggcaagca tttcgtcctt tcttgttact ttcggctctt cttttttagg
    13561 ctcttccttg atcttctgga tattttttgt aagatcgagc tctcttacaa gaaattttct
    13621 tccggaggct gtgtcggtaa catgaatggc tccgtcctct ttgacatcgc atattacatt
    13681 tttcatgctg tcggccattc caagcccgga gagagcagat tccagagcac agaaccagtt
    13741 gggaccttct tctcttacaa ctacttcttt tcctccctca gccaaaggaa caagaacttc
    13801 aaatttcgtc attttttcac ctcacggaaa gataatatca agacaatgat atgttttctc
    13861 tttttttttt caacaaattt aaggaaatat ttttattcct tttccggttt tattctgact
    13921 ttaccttctt cataaacaac actgaattta aagcggtctc caaatttttt tcttacctct
    13981 tctatctttt tttcaagcat cgatgtaaca gcttccttat caaatgtctt tccagtgtat
    14041 tttttagaca tttttatata tttttctgcc gcttcatcta ttacagaact cattctgaat
    14101 gcatttgcat ctttttttgc caattcatca gcagattttt taatgtcatc catgggggac
    14161 cttccgatgc caagacctcc aaaaaaatga agcccgggtt ttgctctccc ctcttcaata
    14221 tccttaaccc ccttctccca ttttattctg tatgaggtaa aacggtttac gatctgcatt
    14281 gcaagaaatc tttcggcact gtttttaaga tcttgtgaaa ctttcataag ttcatcaatt
    14341 tccttcttaa gtctgttaag cttgtccagt ggcggttttt tttcctgagc tgtaaagtaa
    14401 aaattaaaag aatgttcaag atgttcaatt ttttcagcta gttgagaaat tctttgatga
    14461 aatttcattt aattgaactt ttatttttta gggagattga gatcttcgat ctttatcatt
    14521 tcagcaggtg ttgccacctt tacagcgttg acttcccact gaagctttcc tgtccagttg
    14581 ccttcaatgt caccggcatc gtatcctggg ataattatgt tcttctcttc accgggggca
    14641 agcgggtaaa tctcctcgat ctgtcttgta taaatgagaa aaaatgtttt ctcgaaaaga
    14701 actttctggt ttgcatcgtc cataaatttt acaagaacac ccacataact taaatgcttg
    14761 tctgagttat tctttacagt tgcgtccatc atgtactggc tgagcccgtt gccgaattca
    14821 acttcaccgt tacggacatt tgtaacttcg atcttgtcct tataaagctt tgctgtttca
    14881 atgagttccg gatcgatcgg aatttcgatg gagtctcttt tcatttcctc ttttatcttg
    14941 tctacccttt ccttgagatt ga
//
LOCUS       DAOOYB010000049        14893 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_4038_length_14893_cov_9.572045, whole genome shotgun sequence.
ACCESSION   DAOOYB010000049 DAOOYB010000000
VERSION     DAOOYB010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 14893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14893
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4038_length_14893_cov_9.572045"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..992)
                     /locus_tag="PL056_04510"
     CDS             complement(<1..992)
                     /locus_tag="PL056_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016523519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HPM46307.1"
                     /translation="MNLKDFKSGRYEKGYQYQYFVPEKINHSFSWDDETINYLLEKAS
                     FKLGELNSFSRFVPDIDMFIKMHIFKEAVVSNRIEGTRTNIEEALNNENDINPEKRDD
                     WKEVNNYVSAMNSSIENLKTLPISSRLLKDTHKVLLSNVRGEHKNPGEFRTSQNWIGG
                     ASIADAVFVPPVHTELPKLITDLDLFLNNDELKIPYLIKIAIAHYQFETIHPFLDGNG
                     RIGRLLITLYLVSAGILEKPLLYLSEFFEKNKGLYYDNLTFVRTKNDITQWIRFFLTG
                     IIQTAENSVETLRKIMDLKSRIEKEEIVSLGKRSQQGMNLFHKLFKNPVVSVKTV"
     gene            complement(1177..3510)
                     /locus_tag="PL056_04515"
     CDS             complement(1177..3510)
                     /locus_tag="PL056_04515"
                     /inference="COORDINATES: protein motif:HMM:NF020269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GON domain-containing protein"
                     /protein_id="HPM46308.1"
                     /translation="MKKVAFFILLLILTGCEQGKNDSVKVVSSRPDVFKVCLDLDDSR
                     KCEESEPIFVTDEKGMLQKSFLSEYSQAVNLVVTEGSVTGRMYLISDVNSDVISPATT
                     LLMFHDLSGSAGSLEEFFKEEKKIQQISELYLELFKMFSAKGLESYDSGKKAYEMIID
                     KGDSVVLKDGLSSKEPGTYIADPLVLTFRQGFQHGAGLNALSGELTAVNSCIKETASV
                     KTAELNETLLSIDLVTSIDEFLKSMEIELRISFDSINVSGGIYGKFKDISDRDSNSVF
                     AVVKIMAKIADYNIENVELTDEAVKHYVDNYDVFREKCGDLFLNTVTTGGAYIGILEI
                     KTGSAYEKLQLEAALRGAYKPMHLEIEASLAYLKEKLSIVSNYTMHVLSTGGISDVIT
                     HPSVDSNDKILKSFLENGQKFANALYENYKNGKGVSVNNFTARFEPYHQIINIAPPGL
                     TEAANRNEAISQYDDIYNSYSTVLLSTREILARPWDYEEGKEREQELRETESDLEWFL
                     ALVKTTAVMCGKERNDCTYPEDFLKENVMEPALKIENGLPVEKSLYPRNCVERKRYFA
                     NRNDEDVYTLFMGGDKNSPFSVYCENMDGDDPQSYLILNNFSPLPAPESNNDLSPMYN
                     FSTRYISGATYEEKAKEVTVYRRIKVKINYDNLTIMPSQDKFFESVIMNNFSNMKAER
                     LSPGTATVCGDTGFFDAENNRHLPVVANINVAGTQFCFPDGENRFYVTGYNPPPDVEM
                     VVENTSDNISIMLSNNTDETCVGVEPLQDIILEYCGN"
     gene            complement(3507..5630)
                     /locus_tag="PL056_04520"
     CDS             complement(3507..5630)
                     /locus_tag="PL056_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46309.1"
                     /translation="MYARLKIFLVFVFVVHIPACFDPGIGGGVTVTEIQDNALFSSGE
                     IFYIPGMRFGSGFKKGVGSLISQADCLNEYKITKINERNDTIFKLTFIENAQHFKDLV
                     YFRGKMSGVITTQAGPIKLTGETKLSNYIKQNSNNVFIMVEIEAELSDYAVERPVMKI
                     EDGDISGDKEGTLRNLYKKDYSKFVEKCGDVFLSTITTGGRYIGVIEIHTDSQYKKNQ
                     VTLELSGKYIPLQLTVTGGLNVFVETLASKYQMNIHVISRGIPSHEKIVESLSKEHLK
                     ADNQLDDAKNDDKLDENPGPNIDVAPLVKSDIKLFLDDVQNFHDKLKEYEKTSWEYSA
                     YNARFSSYASTAPEIERGTGSILSQNREDANEQYKDIYNNYMELLEKIEDIKFFPEKY
                     FSDETGVASLIDDFYLKETDIRNSIVDLENIILQCNNNENMCIKIDFADQPDGLNMVD
                     VFAELEALPVKDAVYPATCRELQESYDVTEGDNVILYLGHDKNKPWNAFCSSPEEIYS
                     GSEITFSNMPGFLQEYLVIENLSTVESDSMRYNFSYAEYDRMDYVPSDADNESPDEDE
                     VLITRINAKSAYRKLKVAVNSDHLAVESYQSSEVDEVLDDQISIAGNTNSFFGSVRTC
                     SDRNIYIDGDHEIKPGANADLNGTPFILSEKINYSVFPQNILEDNYNFIFSSDRKILN
                     IYLTSDDGSCVRAEHNGDFILEYEK"
     gene            complement(5635..7461)
                     /locus_tag="PL056_04525"
     CDS             complement(5635..7461)
                     /locus_tag="PL056_04525"
                     /inference="COORDINATES: protein motif:HMM:NF020269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GON domain-containing protein"
                     /protein_id="HPM46310.1"
                     /translation="MFFLFLVFVGAGVFAEVPATPWDQGMGFGTGIDVLTGKLKTASE
                     AVTWNSIDEPVCHHERELEFRLISDKNELQSTVGFELGAKIDVIVATASGSIGATYAN
                     DKSETSIFILIKASHKECSYNMNSPEMKKDAQTLYFSDNNAFRTKYGDAFLSTITTGG
                     TFYGVAEIKHKTVEDKAAFEVQLKAKVLGVTVFKKVIRKTLYNLSKTANISFDVIVNG
                     HIQSTSSVDYFFRQYDNFLTETASEKCQGSSGYMKCPYKTGYFEDYSILTKETFSDAV
                     LDTAKLNITLMENKMSALLDILAALEKVKKNKDGYFFQRSKTTGESFSEIFKDVEKQV
                     KDLYLETFNKNRSCRNNYFVKEGNLKKLNSDCEIENSWSYMDDIKEINKNLPQKKLPS
                     DCMELQNNYKMKFDGVYTLYVNNEGHRFFKAYCRNMDSEFPAETYLLLPAISPVSGNP
                     DHNYSKYVNYMHETLKTETTLTTVFHGYLIEKYSEDVYEIVMGQNFYLASHGGSIKVK
                     TAYGGTDILYFTVAGYPSVCNNSDELDESIRRIANMNLSGSPFAFDIDESDFKIYGNL
                     KDAQVEITMDAQKKNLEIKKTKIGPDSCIAVRAERLLIKYGK"
     gene            complement(7494..11228)
                     /locus_tag="PL056_04530"
     CDS             complement(7494..11228)
                     /locus_tag="PL056_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46311.1"
                     /translation="MTKRSMKADFLKFAEIIVLTVLFVLVSGCDTKEESLGGEEEGRV
                     ITQTYISNANVCLDINMNFRCEKSERTVATDASGFFSVTALSLTEDEIEKYQLVAELH
                     SESEYVGYGRKTGGNVLSSIPGHPGFITPLTTIIAVETGRTGGTSIEEAENKFKEAFG
                     LPNYTYFKSDYVVEENDTLNTIASFISAHQKENLADVYNDKEITSLRFHLEQVNDDIF
                     ENAQVIQALSSTPEDNTRQGKGQVNLDPKVLSNLPFTQGMDFGVGYDKLTSNILTASQ
                     CIESNLEPYKDSTGRLPASFYEDKINIGRMVVQRTAERNEVDYFFQVVESKQDLYDMF
                     SLDASIDLNIGEFAGSLQGSYAKEMENDQSSVYVLIKVDAKLADFKIVNPTLTTEAVD
                     YFLCNSTHLKGCVDNDYTQGYNPENFRKKCGDYFLNVITVGGSYYAMLKIKTNSLQEK
                     KEISGKLQASYKSKESGTTGNVSGEFSYVIDNLTENYNATIHTITKGVPGSLSAVKSK
                     DDLMKDIDAFFKNLSENAGYPQVSFDDEKTEEKETTPFVPSIDYRKAVFEVKFEDYEQ
                     SFMDNIKGGADEITVDALKKVALDKEIKDTIIELYSRYEHAETTIRFMLAFPESFIGV
                     IERDKELGEMLKKLRAFKSITKFFAYECTKTVGRSCKLITQSACDDATTEAGTCTDNE
                     EIVPELYSVANVLWFKDECDVDKPQMCLERPETIVNSLPAERIVYPATCVQRKKLYAT
                     NDKSGIYTLFMSSDKEKPYLIYCDDMDVSEEECNPTGSEDSEESPTQADYDINCGPKE
                     YLILSNISPQPGSTADPTLFTPSYNFSRYVGYSGDDGSGLETMLTSVYYKLRVLVNRS
                     HLAVVPDQEMFVSTIGDMPEGIDGSTARYGSTSACSLNKEWSAKAGGVQGYLTRISAN
                     ISLEGTPFYLPDDLTFITNKQFDVVNTAASHDAAYKNAVSKGGYLPVVQDEEDNNKFK
                     SIINTLGMKGSWMGLKSEGTKALIEVFVAEKDDKDCQEGENKIIKGIDKNSNGKLDNL
                     EIQSVVCTAEAVGEIDAATDCGVEGGKKRTVGASEIKLCNTSFQTGFKGFHWLDTTIP
                     FNESHNYFVYDDDASRNKDENELMCGFMSGHPRSYPLINAGGTDFEDVATTVDKNWFE
                     FPCSGEITAASGTQVYEYPYIIEYLSLSTEIEDIDYKFTPDGSTTEKTLRQGVNLKIS
                     NKLDNGKCGYLRPAYDLILEYDKDLADHLGIIEKAEIPGE"
     gene            complement(11255..12529)
                     /locus_tag="PL056_04535"
     CDS             complement(11255..12529)
                     /locus_tag="PL056_04535"
                     /inference="COORDINATES: protein motif:HMM:NF012632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46312.1"
                     /translation="MGKLTYLIIFVFFVSSCGDLNVGHGQSVLSDDDIDENVIPDLIE
                     EIDEDISYDEEIDIDDNEIVTDDGTVITDDEKSVFWEAVSAGEEHTCAIADGSIYCWG
                     KNTYGQLGNGTTEDSDIPVRAGSGSNWKSISSGRTHTCALNAASELYCWGDNWAGQIG
                     TGTEDNESAPLKIGFKNDWTFISSGDGHTCGIAAGRLYCWGDNWAGQVGDGTEIMRRI
                     PVKIGENSDWSVVSAGYYYSCGVQGGKVFCWGKNSSGRLGDGSGEDALSPVRVGDKTS
                     WTTVSAGFGSHTCGISKGKVFCWGANTYGQLGTGTLTNSDLPEIVPDVSDWQRVSAGN
                     DHTCGISKTVLYCWGDNYFFQLGDGTTENSLNPEKSSQAAVYQSVSAGFNHSCAIAGG
                     ALYCWGNNKSGQLGDGTKTDRENPVLIKIKAN"
     gene            complement(12531..14654)
                     /locus_tag="PL056_04540"
     CDS             complement(12531..14654)
                     /locus_tag="PL056_04540"
                     /inference="COORDINATES: protein motif:HMM:NF012632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46313.1"
                     /translation="MKFQVCILIICSTLIFAVSCGDTDTRTISDENVTIFKTFEEAPG
                     ENCLNGGIKIVSGHDENGDGELTEKEILSTDYVCNGDNGYDGNDGSNGTDGANGDQPL
                     VRTSVEPSGENCLEGGTKIETGMDENGNGELDDAEVDSTLTQYVCHGLEGADGQDVLV
                     KTTNEPAGENCTEGGTKVEIGIDENGDGNLDEEEIDPDLTKYICNGQKGACSDNHVPE
                     ISEVYVDDTLYSGSALPVKPKEEITVKVKITDEDAEDAHSVTISGYGAEITGNTSTDV
                     EVDEDGRYVFKITVSALGLFNFSIMVTDGCNIATGTVTMVSSNVKMTTVSAGEGHTCA
                     LNNFGAAYCWGKNNAGQLGNGSTDESTLPVAVNMSNLPEGKKFTAISAGKIHTCALDN
                     DGKAYCWGNNTNGELGDNTDTIRLIPVAVNTTGALSGVVLTVISAGETHTCSGDGSNV
                     YCWGGNSHGQLGNGASGAGVVSKVAVKVQDSAGDLGSVTNISSGGYHTCAWNKNEAGK
                     AFCWGFNNYGQIGDNSSDVDREKAVSIDFSGVISGKTIAQISAGYWHTCAVDSDGKVY
                     CWGHNFYKQTGTDSSGNDVLSPVVVNENSVLTGKTVVSVSTGRSHTCSVDDKGDVYCW
                     GWNKYGQLGNGSTEDSGSLAVKVDKDNSALKDIKADGIYLGYDHSCAVSVTEFEGNVY
                     CWGSNSSGQLGNGNKIDTNLSVEVK"
     gene            complement(14736..>14893)
                     /locus_tag="PL056_04545"
     CDS             complement(14736..>14893)
                     /locus_tag="PL056_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018034316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HPM46314.1"
                     /translation="IGFKELGDNGFGYDPLFIPDGCDKTMAELSSDIKNSLSHRGNAF
                     RKLMEKL"
BASE COUNT         3920 a         3378 c         2628 g         4967 t
ORIGIN      
        1 actgttttta ctgagacaac ggggttttta aaaagtttgt gaaaaaggtt catgccctgt
       61 tgtgatcttt tccccaaaga aacaatctct tctttttcta ttctgctttt taaatccatt
      121 atttttctta gcgtttcaac tgagttttcc gctgtctgaa taatgcctgt cagaaagaag
      181 cggatccatt gggttatgtc attttttgtt cttacaaatg tgagattatc ataatacaga
      241 cccttgtttt tctcaaaaaa ttcagataga tacaaaagcg gcttttcaag aattccagca
      301 gaaacaagat aaagtgtgat aaggagccgc ccgattcttc cattcccgtc aagaaaaggg
      361 tgaattgttt caaactgata atgtgcgatt gcaattttta tgagataagg aatcttcaat
      421 tcatcattat ttaaaaacag gtcaagatct gtaataagtt taggtagttc cgtatgaaca
      481 gggggaacaa agacagcatc tgcaattgat gctcctccaa tccagttctg tgatgttctg
      541 aattcccccg gatttttatg ttcacctcta acatttgaca acaatacctt atgagtgtct
      601 tttaaaagtc ttgaagaaat cggaagggtt ttaagatttt ctattgagga attcattgcc
      661 gaaacatagt tgttgacttc tttccagtca tctcttttct caggattgat gtcattttcg
      721 ttgttcagcg cctcttcaat atttgttctg gttccttcaa ttctgtttga tacgacagct
      781 tctttaaaaa tgtgcatttt tataaacatg tctatatccg gaacaaaacg ggagaatgaa
      841 tttaattcac caagtttaaa agatgctttc tcaagcaggt agtttattgt ttcatcatcc
      901 cacgaaaaag aatgattaat tttctcagga acaaaatact gatattgata tcctttttcg
      961 tatctgccgg acttaaaatc ttttaaattc atctccccct ccaaagtaaa acacaaaaat
     1021 ccttaattta cctaagcggc gtaaagtaaa acacagacct ccttatttta ctttaatgcc
     1081 tcttcctatt ttaagagttg ccgttgaaat agcaattaat ttgtttattt cacattccga
     1141 agggataaga gttgaagtta agtgatcggt ttcttttcag tttccacagt attcaaggat
     1201 tatgtcctgc agaggttcaa cgcccacaca ggtttcatct gtgttattgc ttaacattat
     1261 tgagatattg tcagaggtgt tttccacaac catctcgaca tccggaggcg gattgtaacc
     1321 ggtgacataa aatctgtttt ctccatcggg aaaacaaaat tgtgtgccgg caacattgat
     1381 gtttgcaacc acaggaagat gtctgttatt ttccgcatca aaaaaaccgg tatctccgca
     1441 cacagttgct gttcccggcg aaagacgctc cgctttcata tttgaaaaat tgttcattat
     1501 cacactttca aaaaatttgt cctgagaagg catgattgta aggttgtcat aatttatttt
     1561 taccttgatc cgtcggtaaa ccgtgacttc tttcgctttt tcttcataag ttgctcccga
     1621 tatgtatctt gtcgagaaat tgtacatcgg ggaaagatcg ttatttgatt caggtgcagg
     1681 taatggactg aaattattta agataagata actctgcgga tcgtctccat ccatgttttc
     1741 gcaataaaca gagaaaggac tgtttttatc tccgcccata aaaagtgtat atacatcttc
     1801 gtcatttcta tttgcaaaat accgcttcct ttcaacgcaa tttctgggat ataacgactt
     1861 ttcaacagga agaccgttct ctatttttaa cgctggctcc ataacatttt ctttaagaaa
     1921 atcttcagga tatgtacagt cattcctttc ctttccgcac atgacggcag tcgttttgac
     1981 taaagcgagg aaccattcga gatcactctc tgtttctctt aattcctgct ccctttcctt
     2041 tccttcttca taatcccacg gccttgcaag aatttctctt gtcgaaagaa gaactgtgct
     2101 gtatgaatta taaatgtcat catactgaga tatcgcttcg ttcctgtttg cggcttctgt
     2161 cagtccgggc ggagctatgt tgattatctg atgatacggt tcaaaccttg cagtaaaatt
     2221 gtttaccgaa accccttttc cgtttttgta attttcataa agggcatttg caaacttctg
     2281 cccgttttct aaaaaggatt tcagtatttt atcgtttgag tccaccgaag gatgagttat
     2341 tacatcgctt atcccgcctg tgctcagaac atgcattgtg tagtttgata ctatcgaaag
     2401 cttttcctta agatatgcaa gcgaggcctc tatttcaaga tgcatcggtt tatacgcacc
     2461 ccttagtgcc gcttcaagct gaagtttttc ataggcactg cctgtcttta tttccagaat
     2521 ccctatgtat gctccgccgg ttgtaacagt attaagaaac aaatctccgc acttttccct
     2581 gaaaacatcg taattatcaa cataatgttt caccgcttca tctgtcagtt caacattttc
     2641 aatattgtaa tctgctattt ttgccattat ttttacaacc gcaaaaacgc tgttggagtc
     2701 tctgtctgaa atgtctttga actttccgta tatacctccg gaaacattaa ttgaatcaaa
     2761 gcttattcta agttcaatct ccatgctttt aagaaattca tctattgaag ttacaagatc
     2821 tatgctcaac agtgtttcgt tcagttcagc cgttttaact gacgctgttt ctttgataca
     2881 gctgtttacg gccgtgagct ctccgcttaa tgcattaaga cccgcaccgt gttgaaatcc
     2941 ctgccggaat gtaagaacaa gcggatcggc aatatatgtt cccggctctt tgcttgacaa
     3001 accgtccttt aaaacaacgc tgtcaccctt atcaattatc atttcataag ctttttttcc
     3061 tgaatcataa ctttcaagcc cttttgcgct gaacatttta aaaagctcaa gataaagttc
     3121 agatatctgc tgtatttttt tttcttcctt aaaaaactct tcaagcgatc ctgccgatcc
     3181 cgaaagatca tggaacatca gcagagtcgt tgcgggagaa atgacatccg agttaacatc
     3241 agatataaga tacattctcc ctgttacact tccctctgta acaacaagat taactgcctg
     3301 agaatattca cttaaaaacg atttttggag cattcctttt tcgtctgtaa caaaaatcgg
     3361 ctcactctct tcacattttc tgctgtcatc aagatcaaga catactttga aaacatcagg
     3421 tcgcgacgaa acgaccttta cgctgtcatt tttcccctgt tcacaaccgg ttaaaattaa
     3481 aagcagtata aaaaatgcga cttttttcat ttttcatact ccagaataaa atcaccgttg
     3541 tgttctgctc tgacgcagct cccgtcatcc gaagtaagat aaatattcag aatttttcta
     3601 tctgaggaaa aaatgaaatt gtaattatct tcaagaatgt tttgcggaaa aactgaataa
     3661 tttatcttct ctgacagaat aaacggtgtt ccgttgagat cggcatttgc ccccggctta
     3721 atttcgtgat cgccgtcaat gtagatgttt ctgtcactgc atgttctcac agatccgaaa
     3781 aaggaatttg tattaccggc aatgctgatc tgatcatcaa gcacttcatc aacttcactg
     3841 ctctggtaac tttcaacggc aagatgatca gaattaacag ccactttcag tttccggtat
     3901 gcactttttg catttattct ggttatcagc acttcatctt catcagggga ttcattatct
     3961 gcatccgacg gaacataatc catccggtca tattcagcat aagagaaatt gtatctcatg
     4021 ctgtcacttt caacagttga cagattttca attacaagat attcctgaag aaaaccgggc
     4081 atattggaaa atgttatttc gcttcctgaa taaatctctt ccggagaaga acagaatgcg
     4141 ttccacggtt tattcttatc gtgaccaaga taaagaatga cattatcccc ttctgtaaca
     4201 tcataagatt cctgaagctc tctgcaggtt gccggataaa ccgcatcttt taccggaagt
     4261 gcttcaagct ctgcaaaaac atccaccatg ttcaagccat ccggctgatc tgcaaaatct
     4321 atttttatac acatattttc attgttattg cactgaagga taatgttttc cagatccact
     4381 attgaattac ggatatcggt ctcctttaga taaaaatcat ctataaggga agcgacgccc
     4441 gtttcatcgc tgaaatattt ttcaggaaag aactttatat cctcaatttt ttcaagaagt
     4501 tccatataat tgttatatat gtccttatac tgttcgttgg catcttctct gttttgtgac
     4561 aaaatagagc ccgttcccct ttctatttca ggggcagttg acgcatagct tgaaaaacgg
     4621 gcgttatatg cggagtattc ccatgaagtt ttttcatatt ccttaagttt gtcgtgaaaa
     4681 ttctgaacat catcaagaaa aagtttgata tccgacttga ctaacggagc aacatctata
     4741 tttggaccgg ggttttcatc aagtttgtca tcattttttg catcatcaag ctgattatcc
     4801 gctttcaggt gctcttttga aagggattca actatttttt catgacttgg gatgcctctt
     4861 gagatgacat gtatgttcat ctgatatttt gaagcaagcg tttcgacaaa aacattaagc
     4921 cctccggtga ctgtaagctg aagggggata tattttcctg acagctcaag ggttacctga
     4981 ttttttttat actggctgtc agtatgaatt tctataactc cgatatatct ccctccggtt
     5041 gttatagtgc tgagaaaaac atctccgcat ttttctacaa attttgaata gtctttttta
     5101 taaagattcc gcagcgtccc ttctttgtct ccgcttatat cgccatcttc gattttcatt
     5161 accggtcttt caacggcata atcagaaagt tccgcttcaa tttcgaccat tataaaaaca
     5221 ttgttgctgt tctgttttat ataattggaa agctttgtct cccctgtcag cttaattgga
     5281 cccgcctgag tggtgattac tcccgacatt tttcccctga aatagacaag atctttaaaa
     5341 tgttgcgcat tttcaataaa cgtcagctta aaaatggtat cgttcctttc gtttattttt
     5401 gttattttgt attcattcag acagtctgcc tgacttatga gagaaccgac ccctttttta
     5461 aatccgcttc caaatctcat acccggaata tagaatatct caccagaaga aaaaagggcg
     5521 ttgtcctgaa tttctgttac ggtcactcct cctcctatgc cgggatcaaa acatgctgga
     5581 atatgtacaa caaaaacaaa tacaagaaat atttttaacc tcgcatacat gcttctattt
     5641 tccgtacttt atcaaaagtc tttcagctct tacagcaata caggaatcag gtccgatctt
     5701 tgtttttttt atttccaaat tctttttttg agcatccatt gtgatctcta cttgagcatc
     5761 tttgaggttt ccatagattt tgaagtcaga ttcatcaata tcaaaagcaa acggagaacc
     5821 ggaaagattc atattcgcta ttcttctgat gctttcatcc agttcatcgc tgttgttgca
     5881 gacagacgga tatccggcaa cagtgaaata aagaatgtct gttcctccat atgccgtttt
     5941 cacctttatt gatccaccgt ggctggcaag ataaaaattc tgccccatca ctatttcata
     6001 aacatcttcc gaatatttct caataagata gccgtggaaa acagttgtaa gcgttgtctc
     6061 cgttttaagc gtttcatgca tatagttcac atattttgaa tagttatgat ccggattgcc
     6121 tgaaacaggg cttattgccg gaagcagaag ataggtttca gcagggaatt cactgtccat
     6181 atttctgcag tatgctttaa aaaaccggtg tccttcattg tttacataaa gagtgtaaac
     6241 cccgtcaaat ttcattttat agttattctg aagttccata cagtcggagg gaagtttttt
     6301 ctgcggcaga tttttgttaa tttcctttat gtcatccata taactccagc tgttttctat
     6361 ttcacagtct gaatttagtt ttttaagatt tccttctttc acaaaatagt tgttgcggca
     6421 gcttctgttt ttattaaaag tctcaagata aaggtctttt acctgttttt ccacatcctt
     6481 gaaaatttcg ctgaagcttt cacctgttgt ctttgaccgt tggaaaaaat atccatcttt
     6541 gttcttttta actttttcaa gggcggcaag aatatcaagg agggcggaca ttttattttc
     6601 catcaaagta atattcaact tggccgtatc aaggacagca tcactgaatg tctcttttgt
     6661 cagtattgaa taatcttcaa aatatcctgt tttatatgga catttcatat atccgctgct
     6721 cccctgacat ttttcagaag ccgtttcggt taaaaagttg tcatactgcc tgaaaaagta
     6781 atcgactgag gatgtgctct gaatatgacc gttcacaatt acatcaaagg agatatttgc
     6841 cgttttgctt aaattataaa gagtttttct gataactttc ttaaaaaccg tgactccgag
     6901 cacctttgct ttaagctgaa cctcaaatgc ggctttatct tccacagttt tatgctttat
     6961 ttccgcaact ccgtaaaaag taccgccagt tgttattgtg cttaaaaaag catctccgta
     7021 ttttgttctg aatgcgttat tgtctgaaaa atagagtgtt tgagcatctt ttttcatttc
     7081 cggactgttc atgttgtaac tgcattcctt gtgagatgcc tttattaaaa taaaaatgct
     7141 cgtttccgac ttgtcgtttg cgtatgtagc accgatggaa cctgaagccg ttgcgacgat
     7201 tacatctatt tttgctccga gttcaaatcc tacggtgctc tgcagttcat ttttgtctga
     7261 tataagccgg aattccaatt ctctttcgtg atgacagacc ggctcatcta tcgaattcca
     7321 ggtgacagct tccgatgccg tttttaattt tccggtcaac acatcgatgc ctgtgccgaa
     7381 acccatgccc tggtcccagg gcgtggcagg aacttctgca aaaacaccgg caccgacaaa
     7441 gaccagaaac agaaaaaaca accctgctat tttgcccgta tgaaacaaac tgtctattct
     7501 ccaggtattt cagctttttc aatgataccg agatgatcgg caagatcctt atcatattca
     7561 agaatcaggt cgtatgccgg tctcagatat ccgcattttc cgttatcaag tttgttggat
     7621 atcttaaggt ttacaccctg ccttaaagtt ttttcagttg ttgagccgtc aggtgtgaac
     7681 ttgtaatcaa tatcctctat ttctgtgctc aagctcaggt attcgataat atacggatat
     7741 tcatagacct gcgttccgga ggccgccgtt atttcacctg aacacgggaa ttcaaaccag
     7801 tttttgtcaa cagtcgtcgc cacatcttca aaatctgtcc cgcctgcatt tattaaagga
     7861 tatgatctgg gatgaccgct cataaatccg cacatcaact cgttttcatc cttatttctc
     7921 gatgcgtcat catcataaac aaaataattg tgactttcat taaacggaat ggtcgtgtca
     7981 agccagtgaa agcctttaaa ccctgtctgg aacgaagtat tacaaagttt tatttccgat
     8041 gcacctactg ttctcttttt tccaccttcg acaccacaat ctgtcgctgc atcaatttca
     8101 cctacagctt ccgcagtaca gactactgac tgaatttcta aattatcgag tttgccgtta
     8161 ctgtttttat caattccttt aattattttg ttttcccctt cctggcaatc cttgtcatct
     8221 ttttctgcaa caaaaacctc tataagcgct tttgtccctt ctgatttaag tcccatccag
     8281 cttcctttca ttccgagagt gttgattatg ctcttaaact tattgttgtc ctcttcgtcc
     8341 tgaacaacag gaagatatcc cccctttgaa acagcgtttt tataagccgc atcatgcgaa
     8401 gccgctgtgt ttactacatc aaactgttta tttgtgataa aagtgagatc gtcaggaaga
     8461 taaaacggcg ttccttcaag acttatgttt gcagagatcc ttgtcagata tccttgaaca
     8521 ccacctgctt ttgcactcca ttccttgttc agactgcatg cacttgttga accgtatctc
     8581 gctgttgacc cgtctatccc ttcaggcata tcaccgatag ttgacacaaa catttcctga
     8641 tcgggaacaa ctgcaaggtg acttctgttc acaagtactc tgagtttgta ataaaccgat
     8701 gtgagcatcg tttcaagtcc gcttccgtca tctccgctgt atccgacata tcttgaaaaa
     8761 ttatatgacg gggtaaacaa ggtcggatct gctgttgaac cgggttgagg gcttatgttg
     8821 ctgagtatca gatattcctt cggcccgcaa tttatgtcgt aatctgcctg cgtgggactt
     8881 tcttctgaat cctcggaccc ggtcgggtta cattcttcct ctgaaacatc catgtcatca
     8941 caataaatga gatatggttt ttccttatca ctgctcatga aaagtgtata tatgcccgat
     9001 ttgtcatttg tcgcataaag ttttttacgc tgaacacatg ttgcaggata aacaattctt
     9061 tcagcaggaa gtgaattgac tattgtttcg ggtctttcaa gacacatctg cggcttatcc
     9121 acatcgcact catccttgaa ccacaaaaca tttgcgacac tgtaaagttc aggaacgatt
     9181 tcttcattat cggtacaggt tcccgcttca gttgttgcat catcacaggc actctgtgtt
     9241 atgagcttgc acgatcttcc gacagttttt gtacattcat aagcaaagaa ttttgttatt
     9301 gatttgaatg ccctgagctt tttgagcatt tctccaagct ctttatctct ttcaatgaca
     9361 ccgatgaagg attcagggaa cgcaagcatg aaacggatcg ttgtctcggc atgttcatat
     9421 ctgctgtaaa gctcaatgat cgtgtctttt atctctttat ctaaagcaac cttttttaag
     9481 gcatctaccg ttatttcatc cgctccgcct ttgatgttgt ccatgaaaga ctgttcataa
     9541 tcttcaaatt taacctcaaa gaccgccttt ctgtaatcta tgctcggcac aaacggtgtg
     9601 gtttcctttt cttcagtttt ttcatcatca aaggaaacct gcggatatcc tgcgttttca
     9661 gaaagatttt taaagaaagc atcaatatct ttcatcaggt cgtctttgct tttaactgcg
     9721 gaaagacttc ccggaacacc ttttgttatt gtgtgaattg tggcattata attttcagta
     9781 aggttgtcaa ttacatatga aaactctcca cttacattcc ctgtggttcc gctttccttg
     9841 cttttatatg atgcctgaag ttttcctgat atttcctttt tttcctgaag actgttggtc
     9901 tttattttaa gcatcgcata gtaactgccg cccacagtaa tgacattaag aaaatagtct
     9961 ccgcatttct ttctgaaatt ttccggattg tagccctgtg tataatcgtt gtccacacaa
    10021 cctttcaaat gtgtagaatt gcagagaaag taatccaccg cttcagttgt gagtgtcgga
    10081 tttacaattt tgaaatcggc aagttttgca tcaactttta taagaacata aacacttgac
    10141 tggtcgtttt ccatctcttt tgcataagaa ccctgaagac ttcctgcaaa ttcaccgata
    10201 ttcagatcta ttgatgcatc cagactgaac atatcataaa ggtcctgttt gctttcaacc
    10261 acctggaaaa aataatcgac ttcattcctt tcagctgttc tttgcacaac cattcttccg
    10321 atatttatct tatcttcata aaatgatgcc ggaagtctgc ctgtgctgtc tttatacggc
    10381 tcaagattac tttctataca ctgagacgcc gtaaggatat tgcttgtaag cttgtcgtag
    10441 cctacaccaa aatccatacc ctgtgtaaaa ggaaggtttg aaagtacttt agggtcaaga
    10501 tttacctgcc ccttcccctg ccttgtattg tcttcaggag ttgaagaaag cgcctgaatc
    10561 acctgtgcat tttcaaatat gtcgtcattg acctgttcaa gatgaaacct cagagatgtt
    10621 atttccttat cattatagac atctgcgaga ttttctttct gatgagcaga aatgaaagaa
    10681 gcaatcgtat taagggtgtc gttttcctct acaacataat cactcttaaa gtatgtataa
    10741 ttcggcagtc cgaaagcttc tttaaacttg ttttcagctt cttcaatgct cgtgccccct
    10801 gttcttcctg tttcaacagc aattattgtt gtcagaggag tgatgaatcc gggatgtccc
    10861 ggaatagatg aaagaacatt tcctcctgtt tttcttccgt aaccgacata ctcagactca
    10921 ctgtgaagct cagcaacaag ctgatatttt tctatttcat cctcagtaag actaagcgct
    10981 gtgacactga aaaacccgga agcatcagta gcaactgttc tttcagattt ttcgcaacgg
    11041 aagttcatgt taatgtcaag acagacattt gcgtttgaaa tataagtctg agttataaca
    11101 cggccttctt cttccccgcc aagactttcc tctttggtat cacatcctga gaccagcaca
    11161 aaaagcactg ttaaaacaat aatttctgca aactttaaga agtctgcttt cattgatctt
    11221 ttcgtcattt cttttcctcc atatatgtca actgttaatt cgcttttatt tttatcagga
    11281 cgggattttc cctgtctgtt tttgttccgt cgccaagctg cccgcttttg ttgtttcccc
    11341 agcagtaaag agctccgcct gcaattgcgc aactatgatt aaatcctgca gaaaccgatt
    11401 gatatactgc cgcctgactg cttttttcag ggttcaaact gttttcagtt gttccgtcac
    11461 caagctgaaa aaaatagttg tcgccccagc agtaaaggac tgtttttgaa attccgcaag
    11521 tgtgatcgtt acccgcagaa accctctgcc agtctgaaac atctggaact atttcgggga
    11581 gatcgctgtt ggtcagcgtt cctgttccaa gctgtccgta agtgtttgca ccccagcaga
    11641 aaacctttcc ttttgatatt ccgcaggtgt gggaaccaaa tccggctgaa acagtagtcc
    11701 agctggtttt atcacctact cttacaggtg aaagtgcgtc ttcgcctgaa ccgtctccca
    11761 atcgcccgct gctgttcttg ccccagcaga aaacttttcc tccctgaact ccgcatgaat
    11821 agtaatatcc tgcggaaacc acgctccaat cagagttttc tccaatctta acaggaattc
    11881 ttctcattat ttctgtgccg tctccgacct gtcctgccca gttgtcaccc cagcagtaaa
    11941 gtctgcctgc cgcgattccg caggtatggc cgtctcctga tgaaataaat gtccagtcgt
    12001 ttttaaatcc gatttttaaa ggagcacttt cattgtcctc tgtccctgtt ccaatctgcc
    12061 ctgcccagtt gtcaccccag cagtaaagct ctgatgcggc attcaatgca caggtatggg
    12121 ttcgtcctga agagatgctt ttccagttac ttccggatcc tgccctgaca gggatatcgc
    12181 tgtcttcagt tgttccgtta ccaagctgac cgtatgtgtt ctttccccag cagtatattg
    12241 agccatcagc tattgcacag gtgtgttcct ctccggcgga aacagcttcc cagaatacac
    12301 ttttttcatc atctgtgata acagtaccgt catctgtaac aatctcattg tcatcaatat
    12361 caatttcttc atcataactt atgtcttcat caatttcttc aatgagatca gggattacat
    12421 tttcatcaat gtcgtcatca gacaaaactg actgaccgtg accgacattc aaatccccgc
    12481 aggaagaaac aaaaaacaca aaaattatca aatatgttaa ttttcccatt ttatttaact
    12541 tcaacagaaa ggtttgtatc tattttattt ccgttaccga gctgtccgct tgaattgctg
    12601 ccccagcaat agacatttcc ttcaaactca gttactgaaa ctgcacagct gtgatcatat
    12661 ccaagataaa taccgtctgc tttgatatct ttaagggcac tgttgtcctt atcaactttc
    12721 acagctaaag atccgctgtc ttccgttgac ccgtttccaa gctgtccata tttattccag
    12781 ccccagcagt agacatcgcc cttgtcgtca acagaacagg tgtgtgaacg gccggttgat
    12841 acactcacaa cggtctttcc tgttaaaaca ctgttttcat ttacgacaac aggagacaga
    12901 acatcatttc cggaagaatc ggtaccggtc tgtttgtaaa aattgtgtcc ccagcagtaa
    12961 acttttccat cactgtcaac agcacaggta tgccagtatc cggcagagat ctgagcaatg
    13021 gtctttccgc tgataactcc ggaaaaatca atacttacag ctttttctct atcaacatca
    13081 cttgagttgt cgccaatctg tccgtaatta ttaaatcccc agcagaatgc ctttccagct
    13141 tcatttttgt tccatgcaca agtgtggtat ccacctgaag aaatatttgt cacacttcca
    13201 agatccccgg cactgtcctg aactttgact gcaactttgc ttaccacacc tgcacctgat
    13261 gcaccgttgc cgagctgacc atgcgagttg ccgccccagc agtagacatt gctgccgtca
    13321 ccggaacagg tgtgtgtttc tcccgctgaa atcacagtga gaacaacacc gcttaatgca
    13381 ccggtcgtat tgacagcaac aggtatcagt cttattgtgt cggtattgtc accaagttct
    13441 ccgtttgtgt tgtttcccca acagtatgct tttccgtcgt tatcaagagc acaagtgtgg
    13501 atctttccgg cagaaatggc ggtaaatttt ttcccttccg gcagattgct catattaact
    13561 gcaacaggaa gagtgctttc gtcagtggag ccgttgccaa gctggccggc attgtttttt
    13621 ccccagcagt atgcggcacc gaaattgttt aatgcgcaag tgtgtccttc acctgcagat
    13681 acagtcgtca ttttcacatt ggatgaaacc attgtcactg ttcctgttgc aatattgcat
    13741 ccgtctgtga ccatgatcga aaaattaaaa agccccagtg cgcttactgt aattttgaaa
    13801 acatacctgc cgtcttcgtc cacttccaca tctgttgatg tatttcctgt tatttctgca
    13861 ccgtatccgc ttatggttac tgaatgtgca tcttcagcat cttcatcggt tatcttgacc
    13921 tttaccgtga tctcttcttt tggtttcacc ggaagagcag aacctgaata taaagtgtcg
    13981 tcaacataca cttcgcttat ttccggaaca tgattatccg agcaagcccc tttctgaccg
    14041 ttgcagatat attttgtaag gtccggatct atctcctctt catcaagatt tccgtcaccg
    14101 ttttcatcaa ttcctatctc aacttttgtc cccccttcag tacaattttc acctgcgggt
    14161 tcatttgttg tcttcacaag aacatcctga ccgtccgccc cttccaatcc gtgacagaca
    14221 tactgggtga gagtgctgtc aacttcggca tcatcaagct caccgttacc gttctcgtcc
    14281 attcccgttt ctatttttgt ccccccttca agacagtttt cacctgacgg ctcaacagat
    14341 gttctgacta gcggctgatc tccgtttgcg ccgtctgttc cgtttgatcc gtcattgcca
    14401 tcatatccgt tatctccgtt gcagacataa tctgtagaga ggatctcttt ttcagtcagt
    14461 tctccatcac cgttttcatc gtgaccgctg actattttta ttccgccgtt taaacaattt
    14521 tctcccggcg cctcttcgaa tgttttgaat attgtaacat tttcgtctga aattgttctc
    14581 gtatccgtgt ctccgcaact cacagcaaaa ataagagttg aacaaataat taaaatacat
    14641 acttgaaatt tcatcgatgc ctccaattga accgttattg aaattaaccc ttaagaaatc
    14701 aatctgtgag agaatataac atttttttgt tttttttaaa gtttttccat cagttttctg
    14761 aatgcgtttc cgcggtgact gagggagttc ttgatatctg aagaaagttc tgccattgtt
    14821 ttgtcgcaac cgtcggggat gaatagcgga tcatatccga aaccgttgtc gccaagctct
    14881 ttaaatccaa ttt
//
LOCUS       DAOOYB010000050        14696 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_4118_length_14696_cov_13.960795, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000050 DAOOYB010000000
VERSION     DAOOYB010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 14696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14696
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4118_length_14696_cov_13.960795"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..1017)
                     /locus_tag="PL056_04550"
     CDS             complement(31..1017)
                     /locus_tag="PL056_04550"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HPM46315.1"
                     /translation="MNLFSFSNHFKNEKDAGLFSGWQWGLIKNSNFSCGSSGTYDFNS
                     HDEISDNTCFDLASLTKVVFTVPVVYELIYEKILNPYDKISGFFNNLSSDITIIELLS
                     HTSGFPAWLPIYEMVEKNLSLEERKKEVIKIISASRTNDRSKCYSDLNYILLGFIIEK
                     ITGKTIDAVFENFKKDNHLNFNISFSPDKRAPKTAFSNLRNSYPTMTVEDENCHFLGG
                     KCGHAGLFASSSSVVKYFDKLLNLQWFSSISKSLQYAGFDRPEGSDSNYGKKADNSFI
                     GHLGFTGTAVLIDPEKKTVAALLTNCTHPNPEKPQRKERIKKCRQIFFDEVF"
     gene            1160..1309
                     /gene="rpmH"
                     /locus_tag="PL056_04555"
     CDS             1160..1309
                     /gene="rpmH"
                     /locus_tag="PL056_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HPM46316.1"
                     /translation="MKSTYNPSKISRKRTHGFRKRMKTADGRKVIKARRKKGRKILSV
                     AIYKK"
     gene            1323..1673
                     /gene="rnpA"
                     /locus_tag="PL056_04560"
     CDS             1323..1673
                     /gene="rnpA"
                     /locus_tag="PL056_04560"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HPM46317.1"
                     /translation="MDFSFTKSDKIRKSRDYSLLKETGSVFKTKLMVFNYSVSERTKL
                     GVIVTKKVGNSVFRNRVKRWIREVFRTERGRFVTPVDLVMIPRQSSLSYEDIKRDFLY
                     FAVKLNGKSSNRIN"
     gene            1645..1857
                     /gene="yidD"
                     /locus_tag="PL056_04565"
     CDS             1645..1857
                     /gene="yidD"
                     /locus_tag="PL056_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012863543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HPM46318.1"
                     /translation="MGKVLIALIKIYRVAFSPFLGKNCRFYPSCSAYMIEAIEKKGAF
                     KGFFLGIWRILRCNPWNKSCGYDPVK"
     gene            1869..3452
                     /gene="yidC"
                     /locus_tag="PL056_04570"
     CDS             1869..3452
                     /gene="yidC"
                     /locus_tag="PL056_04570"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HPM46319.1"
                     /translation="MENKNFIIAVVLSVAVMFIWSSFFAPQPEPVPEISEEQKSDSVT
                     EKPSAVAEKVDQEQVKTQELQAVVPTTDIGHPEKTGVIKNGSLELGYSSVTGKITRSL
                     IIKEKYLSKNVDLSQALVKDSFFPEMSSVFGKEPEYEVIAADSSSVSFKYENGGVAEI
                     KTIKLLEDFKIVVEKTVTNNSTGTISWKPTLSFNSQHDNKDILSAYNRTFNVIAHKQG
                     AGFEEMEDKEDIEKFFSTGNEVDWIGINYGYFIFAVISPDKKFSASAEIDSSKNSSFF
                     KSESASKVLQPGESHTEKFTVFYGTKEMELLKEEGVGLEKSVTFGWTGFLAEPLLIAL
                     NFFNGFIGNYGLAIIFLTIVVKLLMFPLSNASYKSMSKMRVLQPKIKELQEKYKKDKE
                     TLNKETMLLYQKEGVNPLGGCLPMFIQMPVYIALYYMIQNAVELYNAPFLPFWLTDLS
                     EKDPFYIIPVSLGLLMFLQTKLTPQTMDNAQAKIMMYTMPVVFTWISLVLPSGMTLYW
                     FVNTLLGIAQQLYVNKKYS"
     gene            complement(3546..3767)
                     /locus_tag="PL056_04575"
     CDS             complement(3546..3767)
                     /locus_tag="PL056_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46320.1"
                     /translation="MKFLAIVIVSLLFAVSCGSAEKKADPAPAETAPAATEAPAEVAP
                     AEEAAPAEEAAPAEEAAPAEEAPAEEAAE"
     gene            3957..4745
                     /gene="truA"
                     /locus_tag="PL056_04580"
     CDS             3957..4745
                     /gene="truA"
                     /locus_tag="PL056_04580"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HPM46321.1"
                     /translation="MYVLTISFCGRNYHGWQFQENAVSVQGVLKNTLSEIFRKECPYP
                     SGCSRTDTGVHALEFIATVPALQNIPAESLKKGLNSLLPDDIRILSVEKKDNFQDGRE
                     FVFGKHYRYLICTKPAHSPFSSDLSWHSAYELDIQKMEHALQYFTGTHDFASFMASGS
                     DVKTTVRTIRKTRIFKTDGYIFIDFAGDGFLKHQVRIMSGTLVGVGKGRIEPDSIPSL
                     INAKDRNVVPHTLPGKGLYLYKLFKSTGEMLSYDFPDTFQDMVW"
     gene            complement(4747..5817)
                     /gene="ribD"
                     /locus_tag="PL056_04585"
     CDS             complement(4747..5817)
                     /gene="ribD"
                     /locus_tag="PL056_04585"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HPM46322.1"
                     /translation="MTSSDLKNKMMTLAVELSKKCTTSTYPNPKVGAVVFNDEGAVLA
                     EGFTSCYGGDHAEIDALKKIDFNASGLNMAVTLEPCNHFGKTPPCSQAILKAGIRKVF
                     IAKHEENGKACNGACFLEERGVEIEFMDEFSADVEEINRFFFKNIRTGLPWVTVKVAV
                     SSDGFITEKEGTPSKITGDASKVYVHQLRAQHMAIAVGAKTVNSDNPLLTVRFADGLS
                     PRPVIFSRNLSLNLNAEVIKSSPVIITSSSDTEIKDKLERSGAVVETLKNGFSVYDAL
                     SSLFKKYCLNSIMVEGGASLIKSFIIEKAVDEFQTNTSPKKLVNGVKLFDNESLHVFG
                     NDFQLKGEMFLGEDLLKIYRKK"
     gene            complement(5804..6826)
                     /locus_tag="PL056_04590"
     CDS             complement(5804..6826)
                     /locus_tag="PL056_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46323.1"
                     /translation="MLPFIFALFLSTISSEDSSICQNREVAVEETLYKEILLSSNDFK
                     VKRKYLEKFGHCFQKTGINLIEIKNIPVLEMLDCEKPLKRPGSDVFFSKDGTLCSRSV
                     MFFPSRKVFTINESAQVSSVSGQWKNINNGETILFYNSGASFEENADPSGRKLRKSGD
                     DHYTDQMGKPEHPQFIYESGDWKTVYPLRETFDLRISDDRSFEINMITESPVSYEPEK
                     LIETTGLKASGINIETPVPSNYFIRGKNIKGVRIPRLVPVNSEEGYHGTYVIRITHSA
                     LNSKTSVNISEKNYSISASNSAGKSEFILTVKQKKLTSGELTKILAVLQKQIPSFYIV
                     KENDKQ"
     gene            complement(6826..7185)
                     /gene="rplS"
                     /locus_tag="PL056_04595"
     CDS             complement(6826..7185)
                     /gene="rplS"
                     /locus_tag="PL056_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012765795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HPM46324.1"
                     /translation="MVKQLVQKLEEQQKRIDHYYVKPGDKIEVHTRIIEGEKTRIQVF
                     KGDVIAIKNGRNNNRTTITVRKFSGGFGVEKIIPLHSPMLEKIELVNRGLVRRGKLYY
                     QRLRKGNAARIKPKNVW"
     gene            complement(7218..7766)
                     /locus_tag="PL056_04600"
     CDS             complement(7218..7766)
                     /locus_tag="PL056_04600"
                     /inference="COORDINATES: protein motif:HMM:NF021455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPM46325.1"
                     /translation="MKHFYVALIHHPVIREGSVITSSVTNLDIHDISRVCSTYGLGGY
                     FIVHPDEKMRSIASRLSGHWTDGAGKKNNPDRCEAIKILKIVESLEEVIEKIKSLENR
                     EPLIVGTTARKRKNSITISDLKKKKNLPVLILFGTAGGIDDNYLDSLDFILEPIDAGS
                     GYNHLSVRSAVSIFIDRLYVAE"
     gene            complement(7763..8470)
                     /gene="trmD"
                     /locus_tag="PL056_04605"
     CDS             complement(7763..8470)
                     /gene="trmD"
                     /locus_tag="PL056_04605"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HPM46326.1"
                     /translation="MKIDVLTLFPEFFQSPLSVSMMKRGVENGAVSILMHQIRNFGIG
                     KRRQVDDSPYGGGAGLVMKPEVITAAWDAVPKNDKTLTLLMSPRGKVFNQQMAADLSQ
                     NCEHLIIVCGHYEGIDQRFIDISGAVEVSVGDYILTGGETAAVCIIDAVVRLLPGVLG
                     NEESSKNESFSIPLLEHDQYTMPRVFRGLKIPKVLTTGDHAKIESWRIRNSLKITAER
                     RPDLMSSLPPSFWEGLK"
     gene            complement(8472..8972)
                     /locus_tag="PL056_04610"
     CDS             complement(8472..8972)
                     /locus_tag="PL056_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46327.1"
                     /translation="MRSGDSRGLIDGNRLIVVGQFIKPVKRDGTMLFKPFFTDSGFIL
                     DLKHLYHETFGTKWDVVSIQQIAKGFHIKLKGVNDTSDAGFFSGESVCIYRNEIKGEP
                     VDLLIGEKVYSRDSEMIGVIVSFSKTPSYLLFEIAGNDGEKFFVPFTEEFIVVENSEI
                     RLIKEP"
     gene            complement(8945..9178)
                     /locus_tag="PL056_04615"
     CDS             complement(8945..9178)
                     /locus_tag="PL056_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014958697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HPM46328.1"
                     /translation="MKKFVETIVKQLVDNPDKVSVTEVDGERTTVIELRVAKEDLGKV
                     IGREGRIAKAIRVLLSASTSREQKKCVLEIVED"
     gene            complement(9207..9449)
                     /gene="rpsP"
                     /locus_tag="PL056_04620"
     CDS             complement(9207..9449)
                     /gene="rpsP"
                     /locus_tag="PL056_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HPM46329.1"
                     /translation="MSVSIRLTRGGSKKKPSYRIVVLDSRKKRDGDYLDKLGHYNPCV
                     NPAEIVVDTEKLESWISKGAQCSDTVASLVRKIKKG"
     gene            complement(9473..10813)
                     /gene="ffh"
                     /locus_tag="PL056_04625"
     CDS             complement(9473..10813)
                     /gene="ffh"
                     /locus_tag="PL056_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HPM46330.1"
                     /translation="MFDQLTEKLESTFKKLRGVGKLTEENISEGIRELKLSLLEADVN
                     FKVVKELIEKVKERALGADVMKSITPGQMFIKIFHEELVSALGEGDHSLNISVRPPAV
                     ILMAGLQGSGKTTTAAKLASMLKNKQKKRVVLVPADVYRPAAIDQLKKLGSQIGVDVY
                     PSETGMDPVDIAKKSIEFGKNIVADVIIIDTAGRMQIDVDMMDEITNIKNAVEPSEIL
                     FVADAMTGQDAVNVAAEFHRRLTITGIVLTKMDGDARGGAALSINAVTGAPVKFVGVG
                     EKIDQFELFHPERIAGRIVGMGDILSLVEKAGEKINAEEAMRLQKKMVKNSFDLEDFL
                     SQMKMIKGMGPMEDMLGMIPGIGKEVKKIQGKVNFEKELSRIEAIIFSMTKEERRKPE
                     ILNASRRRRIANGSGTRVQDINQFMKQYLEMKKMMSNLNRFGLGGLMKKFKGIG"
     gene            11116..>11645
                     /locus_tag="PL056_04630"
     CDS             11116..>11645
                     /locus_tag="PL056_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HPM46331.1"
                     /translation="MTRKKVLIMGAAGRDFHNFNTYYRNNKEYEVVAFTATQIPDIDG
                     RKYPAELAGELYPQGIPIYDEADMPRLVKELGVTEVVFAYSDVPYNYVMNRSARVNSL
                     GADFILLGYDKTKVKSSKPIISICAARTGCGKSQTTRFIYDIFKKMGKKVVAVRHPMP
                     YGDLVAQKVQRFAEIA"
     assembly_gap    11646..11745
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11746..12303
                     /locus_tag="PL056_04635"
     CDS             <11746..12303
                     /locus_tag="PL056_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HPM46332.1"
                     /translation="GEVNVCMADVIVINKMDSATLQDIETVKANCKRLNPNAVVIEAD
                     SALTVSGDIKGKRVLIVEDGPTLTHGEMKIGAASVAATRGGVAEMVDPRPFAVGKIAY
                     TYQKYPNIGKGILPAMGYGEEQKKDLETTMNAVDADVVVSGTPIDLGRIVKSNKPIVK
                     VGYELAPRGDGAKKLEEIIVRKLFK"
     gene            12372..13307
                     /gene="arcC"
                     /locus_tag="PL056_04640"
     CDS             12372..13307
                     /gene="arcC"
                     /locus_tag="PL056_04640"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013932733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HPM46333.1"
                     /translation="MKIVIALGGNALIKPGDKGTIEDQERNIASSLDGIIKLIHEGHQ
                     IILTHGNGPQAGSRLIRNEMAEKEVHPDPLVICVADTQGSMGYMIEQGLMNCLSKAGE
                     APKVATLVTQIEVSPDDPAFDKPTKPVGPFYNKDKALEKMNSGWIMVEDSGRGYRRVV
                     PSPMPLKVLNPEALNTLLEAGFTVITGGGGGIPVYRKDDNNFAGIDAVIDKDLASVMI
                     AKTINADMVIILTGVEKVAINFKSGSPTWLDTMTSKEAQEYLEKGEFPAGSMGPKIRA
                     AIDFVKNDSGKTVLITSEDKLTEALEGHTGTKIVQ"
     gene            13356..13928
                     /locus_tag="PL056_04645"
     CDS             13356..13928
                     /locus_tag="PL056_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46334.1"
                     /translation="MKGKMFVVFAGIAFTFILSSCSATLSTSTSSTMSQMKAEPMALW
                     TRPLEVGFTVIGMGEGSSETSQMEGSLSSKKVVDSAPSFFLGGSVPDRSLSPMAKLAA
                     YNAIQKAGADGMYVIMVKEESDGSVKKAWVKGLLLKLGVYGPVSAERADARKCGCGKH
                     SKKEEKNELKPVEEPVMTAPVNDLQSETEK"
     gene            complement(13929..>14696)
                     /locus_tag="PL056_04650"
     CDS             complement(13929..>14696)
                     /locus_tag="PL056_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46335.1"
                     /translation="FIGTWAQKIILKSVSSTSLAKNVPSVTTRYVISEIKLDNDGNLT
                     LDRKNGIMCRTDNRTGDSALNKGVVGFNEPNSKFNTIYHFWKPNQIPGQENMLDAEAS
                     VVGDDIFFKTNKEWELRGANMADPSTESMPTKDNDTRIFDHDNDTKPGFTITFNGFIN
                     GSIHYVQRLTAIFEGKLVSEGKIEGNVEWTDEQYALSSSSAGLKDQKTTVTDKAKSVF
                     QFVKVDDSMDCAALVANLNGANDIFDIADPNTDVPNN"
BASE COUNT         4211 a         2944 c         2998 g         4443 t
ORIGIN      
        1 ttaatccccg cccaggggat ttcctttgat ttaaaacact tcatcaaaaa agatctgtct
       61 gcactttttt atccgttctt ttctttgcgg tttttcgggg tttggatggg tgcagttggt
      121 aaggagtgcg gcaacggttt tcttttcagg gtcgatgagt actgctgttc ctgtaaatcc
      181 gaggtgtccg ataaatgagt tatcggcttt tttcccataa ttggagtcgc ttccttcggg
      241 gcggtcaaat ccggcatatt gaagtgattt ggaaatgctt gaaaaccatt gtaaatttaa
      301 tagtttgtca aaatatttaa caaccgacga actgcttgca aaaaggccgg cgtgtccaca
      361 tttgccccct aaaaaatggc agttttcatc ttcaacagtc attgtcggat atgagttcct
      421 taaatttgaa aaagcggttt tgggagcccg tttgtcagga gaaaaagaga tgttgaaatt
      481 caaatggtta tcttttttaa aattttcaaa aacggcatca atcgtttttc ctgttatttt
      541 ttcaattata aaaccaagaa gaatatagtt caggtcgctg taacattttg atctgtcatt
      601 tgttcgactt gcggaaatga tctttatgac ctcttttttc ctttcttcaa gcgacaaatt
      661 cttttcaacc atttcataaa tcgggagcca tgcgggaaac cccgatgtgt gagataaaag
      721 ttcaattatg gtgatgtcgc tgcttaagtt gttgaaaaaa ccagatattt tgtcgtaggg
      781 attgagaatt ttctcatata tcaattcata aacaacagga acagtgaaca cgaccttggt
      841 caatgatgca agatcaaagc atgtattatc agaaatttca tcatgactgt taaaatcata
      901 agttcctgaa gatccgcagg aaaagttcga gtttttaatt aagccccact gccagccgga
      961 aaaaagtccg gcatcttttt catttttaaa atggttggaa aaagagaaca aattcatttt
     1021 gacacctgaa acaaattcac gctatactac tgatattttt taatttttta aagtttttca
     1081 cttcaatatt tgacaacaca taattcatga gtatatttcc ccgagttttt tgaggtgttt
     1141 tgacagtaag gagtgagcta tgaaaagcac atacaatcca agtaagattt caagaaagag
     1201 aacccacggt ttcagaaaga gaatgaaaac tgctgacgga agaaaggtca tcaaggcccg
     1261 cagaaagaaa ggaagaaaga tcctctccgt agcaatttac aaaaagtagt caaacaagat
     1321 ctttggactt ctcatttaca aagtctgata aaataaggaa aagcagagat tattccttgt
     1381 taaaagaaac gggcagtgtt ttcaagacaa agctgatggt tttcaattat tcagtgtctg
     1441 aaagaacaaa actcggtgta attgttacaa aaaaagtggg caattcagtt ttcaggaaca
     1501 gggttaaaag atggataaga gaagtgttca ggactgaaag agggagattt gtcacgccgg
     1561 tcgatcttgt catgatcccc agacagagca gtttgtcgta tgaagatatc aagagagatt
     1621 ttttatattt tgcggtaaaa ttaaatggga aaagttctaa tcgcattaat taaaatatac
     1681 agagtggctt tttctccttt tctggggaaa aactgcagat tttatccgtc atgttcggca
     1741 tatatgatag aagcaattga aaaaaaaggg gcttttaaag gtttttttct tggaatatgg
     1801 agaatattaa ggtgtaatcc atggaataag agctgtggtt acgacccggt aaaataggag
     1861 ataacagaat ggaaaacaaa aattttatta tagcagtggt tctttcggtc gctgtgatgt
     1921 tcatctggtc atcatttttc gcaccccagc cggagccggt tccggaaatt agtgaggaac
     1981 agaaaagtga cagtgtaaca gaaaaaccgt ccgctgtggc tgagaaagtt gatcaggagc
     2041 aggttaaaac tcaggaattg caagctgttg ttccgacaac ggatatcggc caccctgaaa
     2101 agaccggtgt tataaaaaac ggttcgcttg agcttggata cagttctgta acaggcaaaa
     2161 taacaagatc tctcataata aaggaaaaat atctttcaaa aaatgtcgat ctttcacagg
     2221 cattggtaaa agattcgttt ttccctgaaa tgagttcagt gtttggaaaa gagccagaat
     2281 acgaagttat cgcggcggac agttcttcgg tttcttttaa atatgaaaac ggcggagttg
     2341 ctgaaataaa aacaattaaa cttcttgaag atttcaagat agttgttgaa aaaactgtca
     2401 caaacaattc gacaggaact atttcgtgga aaccaacgct ttcgttcaat tcacagcatg
     2461 acaacaaaga tatactctcg gcttacaaca gaacattcaa tgttatcgcc cataagcagg
     2521 gtgccggttt tgaagagatg gaagacaaag aagatattga aaaattcttt tcaaccggaa
     2581 atgaagttga ctggataggt attaattacg gttattttat ttttgccgtt atttccccgg
     2641 ataagaaatt cagtgcttct gccgagatag attcctctaa aaacagctct tttttcaaat
     2701 ctgaatccgc ttcaaaagtt cttcagccgg gagagagcca cacagaaaag ttcactgttt
     2761 tttacggaac gaaagaaatg gaacttctta aagaggaggg cgtagggctt gaaaaaagtg
     2821 taactttcgg atggaccggg tttttagctg aaccgcttct gatcgcactt aatttcttca
     2881 acggctttat tggaaattac gggcttgcaa taatttttct tacaattgtt gtgaaacttc
     2941 tgatgttccc tctttctaat gcaagttaca aatcaatgag caaaatgaga gttcttcagc
     3001 cgaagatcaa ggagctgcag gaaaaatata aaaaagacaa agaaactctg aacaaggaaa
     3061 caatgcttct atatcagaaa gaaggggtca acccgcttgg cggctgtctt ccgatgttca
     3121 ttcaaatgcc tgtatatatt gcactttact atatgataca gaatgcggta gagctttata
     3181 acgctccctt ccttcctttc tggctcacag acctttcgga aaaggaccct ttctatataa
     3241 ttcctgtttc tctggggctt ttgatgtttc tccagacaaa gcttactccg cagacaatgg
     3301 ataatgctca ggcaaaaata atgatgtaca ccatgcctgt tgtgttcacc tggatatctc
     3361 ttgttcttcc aagcggaatg actctgtact ggtttgtcaa cacactgctc ggcattgctc
     3421 agcagctcta tgtaaataag aaatattcct gaaaaacatc aatagataaa aaaatcccgc
     3481 tcgacatcag aaataaaact ttctaaagcg aacgggaaaa gaatctttta agcagaaacc
     3541 gattattatt cggctgcttc ttcagcagga gcttcttcag caggagctgc ttcttcagca
     3601 ggagctgctt cttcagcagg agctgcttct tcagcaggag caacttcagc aggagcttct
     3661 gtggctgcag gagctgtttc agcaggagcc ggatcagctt tcttctcagc gcttccgcag
     3721 gatacagcaa aaagcagtga tacgattacg attgcaagaa acttcatgtt ttcctccaaa
     3781 aaaatttcaa caaaagtcaa acacgtaata aagccaaaaa cttcatacaa ttcgagaaga
     3841 tatagcacat ttttttaaaa agtccagaaa acctcaaaaa aagcgcaaaa aattttgttt
     3901 cttgactgat gtttccttaa gtgttaaatg gttcagatta ctttttcggg tggaacatgt
     3961 atgttttaac tatttctttc tgcggcagaa actatcacgg atggcagttt caggaaaatg
     4021 cagtttcagt tcagggtgtt ttgaaaaata cattgtccga aattttcaga aaagaatgtc
     4081 catatccttc aggttgcagc agaactgata caggggttca tgcccttgag ttcattgcaa
     4141 cggttcctgc attacagaat attcccgccg aatctttaaa aaaagggctc aactctctcc
     4201 ttcctgatga tataaggata ctgagtgttg aaaaaaagga taactttcag gacggaaggg
     4261 aattcgtttt cggaaaacac tataggtatc tgatctgcac taagccggca cactctcctt
     4321 tttcatccga tctttcatgg cacagtgcat atgaacttga tattcagaaa atggagcatg
     4381 cacttcaata ttttacaggc actcatgatt ttgcaagctt catggcttca ggaagtgatg
     4441 tgaaaacaac tgtgagaaca ataaggaaaa cccgtatttt taaaacagac ggatatattt
     4501 ttatcgattt tgccggagat ggattcctga aacatcaggt aaggataatg tcgggaacgc
     4561 tcgtaggtgt cggaaaaggg aggatagaac ctgattcaat tccttcgctg ataaatgcaa
     4621 aagacagaaa tgttgtaccc catactcttc cggggaaagg gctgtatctt tacaaacttt
     4681 tcaaatcgac cggtgaaatg ctcagttatg attttcctga tacttttcag gatatggtct
     4741 ggtagattat ttttttctgt atatttttaa aagatcttcc ccaaggaaca tttcgccctt
     4801 taattgaaag tcgtttccga aaacatgaag tgattcatta tcgaagagtt ttactccgtt
     4861 aacgagtttt ttcggtgatg tgtttgtctg gaattcatca acggcttttt caatgataaa
     4921 ggactttatg agagaagctc cgccttcaac cattatcgaa ttgagacagt attttttaaa
     4981 aagggaggac agagcatcgt agacagaaaa accatttttt aaagtttcta caactgctcc
     5041 tgatctttca agcttatctt taatttctgt atctgaagat gaagttatta ttacagggct
     5101 gctctttatt acttctgcat taaggttcaa agagaggttc ctgctgaaaa tcacaggccg
     5161 cggacttaaa ccgtcagcaa accttactgt aagaagggga ttatctgaat taactgtttt
     5221 tgcgcctact gctattgcca tgtgctgtgc tctgagttga tgaacataca ctttagatgc
     5281 gtcaccggtt atttttgaag gtgttccctc tttttctgta atgaaaccgt cagaagaaac
     5341 agcaactttt actgttaccc agggaagccc ggtcctgatg tttttgaaaa agaatctgtt
     5401 tatttcttcc acatctgcgg aaaattcgtc catgaattca atttcaactc cgcgttcttc
     5461 aagaaaacat gctccattgc acgcttttcc gttctcttca tgttttgcaa tgaaaacttt
     5521 tctgatccct gctttcagta ttgcctgact gcacggaggt gtcttgccga aatggttgca
     5581 tggctcaaga gtgaccgcca tgtttagacc ggaagcatta aaatcaattt ttttaagagc
     5641 atcgatttct gcatgatctc cgccgtaaca tgatgtgaat ccttctgcca gaacggctcc
     5701 ttcatcattg aaaacaacag cccccacttt cgggttggga tatgttgaag ttgtacattt
     5761 ttttgacagt tcaactgcca gtgtcatcat tttgttttta agatcactgc ttgtcatttt
     5821 ctttaactat gtaaaaagaa ggtatctgtt tctggagtac cgcaagtatt tttgtcaatt
     5881 ctccggaagt tagttttttc tgtttcacag taagaatgaa ttcgctcttt ccggctgaat
     5941 ttgaagcaga aattgaatag ttcttttctg atatgtttac ggatgtttta gaattgagag
     6001 cagaatgggt tatgcggata acataagttc cgtgatatcc ttcttcggaa tttaccggga
     6061 caagccgggg tatccggacc cccttgatat tttttcccct tataaaataa ttgctcggta
     6121 caggtgtttc aatgtttatg ccggatgctt tcagaccggt tgtttctatc agtttttccg
     6181 gttcgtatga aacaggggat tccgttatca tattaatctc aaaagacctg tcatcgctga
     6241 ttcgaagatc gaatgtttct cttaaaggat aaactgtttt ccagtcacct gattcataaa
     6301 taaactgagg gtgttccggt ttacccatct ggtctgtata atgatcgtca ccgcttttcc
     6361 tgagcttcct tcctgaagga tcagcgtttt cttcaaatga tgctccggag ttatagaaaa
     6421 ggatggtctc accgttatta atgtttttcc attgacctga aacagatgaa acctgcgctg
     6481 attcgtttat ggtaaacact ttccttgaag ggaagaacat tacagatctg gaacacaaag
     6541 ttccatcctt ggagaaaaag acatcagatc ccggtctttt caatggcttt tcacagtcaa
     6601 gcatctcaag taccggaata ttctttatct cgatcaggtt tattcctgtt ttctgaaaac
     6661 aatgtccgaa tttctcaaga tattttcttt ttactttaaa atcgttcgaa gaaagaagaa
     6721 tttccttata aagagtttct tcaacagcaa cttccctgtt ttgacagata gatgaatctt
     6781 ccgaagaaat agtgcttaaa aatagcgcaa aaataaacgg taacactacc agacgttctt
     6841 gggtttgatc cttgccgcat ttcctttgcg gagacgctga taataaagtt tccctcttct
     6901 tacgagacct ctgttgacaa gctcgatctt ttcaagcatc ggtgagtgaa gaggaattat
     6961 tttctctacg ccgaatccac ccgagaattt tcttacggtt attgtcgttc tgttgttgtt
     7021 tctgccgttt ttaattgcaa taacatcacc tttgaaaacc tggatacggg ttttttcacc
     7081 ttcgataatt cttgtgtgaa cttcgatctt gtctcccggt tttacataat aatgatcgat
     7141 tcttttctgc tgttcttcaa gtttctgaac taattgcttt accatatctc actccatcaa
     7201 aaatacggac gctgatatta ttcagccaca tacagtctgt caataaaaat tgaaacggca
     7261 cttctgacac ttaaatggtt atagccactt ccggcatcaa tcggctcaag tataaaatcc
     7321 aatgaatcca aatagttatc gtctattccg ccagctgtcc cgaacagtat cagaacgggc
     7381 aggtttttct tttttttaag gtcggaaatc gtaatggaat ttttcctttt tcttgcagtt
     7441 gtccccacaa taagtggttc acggttttca agggacttta ttttttcgat aacttcttcc
     7501 agagattcaa ctatttttaa tatttttatt gcttcacagc ggtcggggtt gttttttttg
     7561 ccggctccgt ctgtccagtg acctgaaagt cttgaagcaa tgctcctcat tttttcatca
     7621 ggatggacaa taaagtaacc gcccagcccg taagttgaac aaactcttga aatatcgtga
     7681 atatcaagat tcgtaacaga agaagtgatg actgatcctt cccttatgac cggatgatgg
     7741 atcaaagcga cataaaaatg cttcatttca gaccttccca gaatgaagga ggcagagagc
     7801 tcataagatc aggtcttctt tctgcggtta tcttcaggga gtttctgatc ctccacgatt
     7861 ctattttcgc atggtcgcct gttgtaagta ccttcggtat tttcaagccc ctgaaaactc
     7921 tcggcattgt atactggtca tgctcaagaa gagggatgga aaacgattca ttctttgaag
     7981 actcctcatt tccaagaact cccggcagaa gtcttacgac tgcatctata atgcaaacgg
     8041 ccgcggtttc tccgcctgtc agaatgtaat cgcccacaga aacttcaaca gctcccgaaa
     8101 tatcaatgaa acgctgatcg atcccttcgt aatgtccgca tactattata agatgttcac
     8161 agttttgaga aagatctgcc gccatctgct gattaaaaac tttccctctt ggagacatga
     8221 gcaatgtgag tgttttgtcg ttcttaggaa cagcatccca tgcggccgtg ataacttcgg
     8281 gcttcatcac aagccccgct cctcctccat aaggcgagtc atcgacctgt cttctttttc
     8341 cgatcccgaa gtttcttatc tggtgcataa gaatagagac agctccgttt tcgacacccc
     8401 gtttcatcat gcttaccgaa agaggggact gaaaaaattc agggaaaagt gttaacacat
     8461 caattttcaa atcatggctc ctttataagt ctgatctcgc tattctcaac cacaatgaac
     8521 tcttcagtga aagggacgaa aaacttctct ccgtcgttac cggcaatttc aaaaaggaga
     8581 taggacggtg tttttgaaaa agaaactatt acgccgatca tttctgaatc acgggaatag
     8641 actttctctc cgatcagaag atctaccggc tctcctttta tttcattccg gtatatgcag
     8701 acggattctc ctgaaaagaa accggcatct gaagtatcgt taacaccttt aagcttaatg
     8761 tgaaatcctt ttgcgatctg ctgaattgaa acaacatccc attttgtccc gaaagtctca
     8821 tgataaagat gcttaagatc caaaatgaat cctgagtctg taaaaaaagg tttgaacagc
     8881 atggtgccgt ccctttttac aggtttaatg aattgtccga caactatcag tctgttgccg
     8941 tcaatcaatc ctctactatc tccagaacgc actttttctg ctcgcgggaa gttgatgcgc
     9001 tcagaagaac tctgatagct ttcgctattc ttccttctct tccgattact ttcccgagat
     9061 cttctttggc aactctgagt tcgatcacag tcgttctttc tccgtcaact tctgtaacag
     9121 agactttgtc agggttgtca acaagttgct ttacgatcgt ttcgacaaat tttttcatag
     9181 cttcctccaa aaaatcagta gaccggtcag cctttcttaa tttttcttac aagcgatgca
     9241 actgtgtctg aacactgggc accttttgag atccagcttt caagcttttc cgtatccaca
     9301 actatttcag cagggtttac gcacggattg taatgtccga gtttgtcaag gtagtcaccg
     9361 tctctttttt tcctgctgtc aagaacaaca atacggtaag agggtttctt ttttgatccg
     9421 cctcttgtca gtctgatact aacgctcatt aaaaatcctc caaataatct ggttatccta
     9481 ttcccttgaa cttcttcatt aatccgccca gtccgaaccg gttgagatta ctcatcattt
     9541 ttttcatttc aaggtactgt ttcataaact ggtttatatc ctgaaccctt gttccgcttc
     9601 cgtttgcaat tcttcttctc cttgaagcgt taaggatctc gggttttctt ctttcttctt
     9661 ttgtcattga aaagattatc gcttctattc ttgaaagctc tttttcaaaa tttacttttc
     9721 cctgtatctt tttaacttcc tttccgatcc ccggaatcat tccgagcata tcttccatcg
     9781 ggcccattcc ttttatcatt ttcatctggg aaaggaaatc ttcgaggtca aagctgttct
     9841 ttaccatttt tttctgaagg cgcatggctt cttcagcatt tattttttca ccggcttttt
     9901 caacgagtga aagaatatct cccattccca ctattcttcc tgctatcctt tcggggtgga
     9961 aaagttcaaa ctgatcgatc ttttcaccga ctccgacgaa tttgacagga gcaccggtga
    10021 cagcattgat agaaagagct gctccgcctc ttgcatcacc atccattttt gtaaggacga
    10081 tgcctgtaat tgtgagccgt ctgtgaaatt ccgcagctac attaactgca tcctgacctg
    10141 tcatggcgtc tgcaacaaaa aggatttcag atggttcgac ggcatttttt atgtttgtta
    10201 tctcatccat catatcgaca tctatctgca ttctgccggc tgtatcgatg atgatgacat
    10261 cggcaactat attttttccg aattcaatgg atttcttagc aatatcaact ggatccattc
    10321 cggtttcaga aggatagaca tcaactccga tctgacttcc gagctttttg agctgatcga
    10381 tcgcggcagg tctgtagaca tcggcaggaa cgagaactac ccgttttttc tgcttatttt
    10441 taagcattga tgcaagtttt gccgccgttg tagtctttcc gcttccctga agacccgcca
    10501 tgagaataac agcaggagga cggacactga tatttaatga atggtcccct tcacccagag
    10561 cgcttacaag ttcctcgtgg aatattttga tgaacatctg ccccggagta atgcttttca
    10621 tcacatcggc tccgagtgct ctttccttaa ctttttcaat gagttccttg acaaccttga
    10681 agtttacatc agcttcaaga agtgaaagtt tcagctctct tattccttca ctgatgttct
    10741 cttcggtaag tttgccaacc cctcggagct ttttaaatgt gctttccagc ttttcagtaa
    10801 gctgatcgaa catacagttc tcctaaacaa aaaagccaaa agattatagt ggagagcacc
    10861 tgttagtcaa gaaattatgt gaatttagct aaattaatca acaaattcaa ggtgttgtgc
    10921 tggtggcgcc tgctgttgac aacaattaag gaaacagttg gttatttaaa acatattaat
    10981 tttacttaag ttttaaaatc atccttgaca tagattttta gtatggtatt ttactttccg
    11041 ccgttgaccc tgtccggccg ccgttgacca agtccggcca ttgtttagca tgatttaaca
    11101 ttttggaggt taaaaatgac cagaaagaaa gttctcatta tgggtgctgc cggtagagat
    11161 ttccacaatt tcaacaccta ttacagaaac aacaaagagt atgaagtggt tgctttcact
    11221 gcaactcaga tccctgacat tgatggaaga aaatatcctg ctgaacttgc gggcgagctt
    11281 tatcctcagg gaattccgat ctatgacgaa gcagacatgc caagactcgt aaaagagctc
    11341 ggagttacag aagttgtttt tgcatacagc gatgttcctt acaattatgt tatgaacaga
    11401 agtgcacggg taaattctct gggtgcggat ttcattcttc ttggatatga caagacaaaa
    11461 gttaagtcat ctaagccgat catttcaata tgtgcggcaa gaaccggctg cggaaagagc
    11521 cagacaacaa gattcatata tgacattttc aagaaaatgg gcaaaaaagt tgtcgcagtg
    11581 cgtcatccga tgccttacgg cgaccttgtt gcacagaaag ttcagagatt tgctgaaatt
    11641 gcagannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnggtga agtcaatgtc
    11761 tgcatggctg atgtaattgt catcaacaaa atggacagcg caactcttca ggatatcgaa
    11821 acagttaagg caaactgtaa gcggctcaac ccgaatgcag ttgtgatcga agcagacagt
    11881 gcacttacag tcagcggtga cataaaaggc aaaagagttc tcatcgttga agacggcccg
    11941 acacttacac acggtgaaat gaaaataggt gcggcaagcg ttgcggctac cagaggcgga
    12001 gttgctgaaa tggtggatcc gagacctttc gcagttggaa aaattgcata cacatatcag
    12061 aaatatccga acattggtaa aggcattctt cctgcaatgg gttacggcga agagcagaaa
    12121 aaagatcttg agacaacaat gaacgcagtt gatgccgatg ttgttgtaag cggaactccg
    12181 atcgaccttg gcaggatcgt gaaatcgaac aagccgatag ttaaagtcgg atatgagctt
    12241 gctccaagag gcgacggtgc aaagaaactt gaagaaataa tcgtcaggaa gctttttaaa
    12301 taaaaatttc tgatcaaact tttgtgaggg ggctttttta agtccccttt tcttttttag
    12361 atggagatcg aatgaagata gtgatagcac tcggcggcaa cgcattgata aaaccgggcg
    12421 ataaagggac tattgaagat caggaaagaa acattgcatc atctcttgac ggcattataa
    12481 aacttatcca cgaaggacat cagatcatac ttactcatgg taacggccca caggcgggat
    12541 cacggctcat aaggaatgaa atggctgaaa aggaagtcca tcctgaccct ctggttatct
    12601 gtgttgcaga cactcaggga agcatggggt atatgataga gcaggggctg atgaactgtc
    12661 tgagcaaggc gggtgaagca ccgaaagttg caacacttgt gactcagatc gaagtcagtc
    12721 cggacgatcc ggcatttgat aaaccgacaa aacctgtagg acctttctat aataaggata
    12781 aggcacttga aaaaatgaat tcgggatgga ttatggttga agacagcgga aggggataca
    12841 gaagggttgt tccctctccg atgcctttaa aggttttgaa ccctgaagca cttaatactc
    12901 tgcttgaagc agggtttact gtaattactg gtggcggagg aggcattcct gtctatcgga
    12961 aagacgataa caattttgcc ggaatagatg ccgttattga taaagatctt gcaagtgtaa
    13021 tgatagcaaa gacgataaac gctgatatgg ttataatcct taccggagtt gagaaagtcg
    13081 ccatcaattt caaatcaggc agcccgacat ggcttgacac catgacttca aaagaggcac
    13141 aggaatatct tgaaaaaggt gagtttcctg caggaagcat gggtccgaag ataagagcgg
    13201 caattgattt tgtaaagaac gattccggaa agacagttct catcacatct gaagacaagc
    13261 ttactgaagc acttgaaggt cataccggga caaagatagt tcagtagata tattaacaag
    13321 ttccatttaa ttgttttttg atcaggaggg agatcatgaa agggaaaatg tttgttgttt
    13381 ttgcaggaat tgcattcact tttattctga gcagctgttc ggcaacattg tcaacatcaa
    13441 catcatcgac aatgtcgcaa atgaaagctg aaccgatggc tttatggaca agaccgctcg
    13501 aagtgggttt cacggttatc ggaatgggcg aaggaagctc cgaaacttct cagatggaag
    13561 ggagtctttc atcaaagaaa gttgttgaca gcgctccctc attcttcctt ggcgggtcag
    13621 ttcctgacag gtccctcagc cccatggcaa agcttgctgc ctacaatgct atccagaaag
    13681 cgggtgctga cggcatgtat gtcatcatgg taaaagagga atctgacggt tctgtaaaaa
    13741 aggcatgggt taaaggcctt cttcttaaac tcggtgttta cggtcctgtc tctgctgaaa
    13801 gggctgatgc aagaaaatgc ggatgcggaa aacattctaa gaaagaagag aaaaatgagc
    13861 tcaaaccggt agaagaacct gttatgactg caccggtaaa tgaccttcag agtgaaacag
    13921 aaaaataatt aattgttcgg aacatcggta ttcggatcag ctatatcgaa aatatcgttt
    13981 gcaccattga gatttgcaac aagtgcggca caatccatcg agtcatcaac tttaacaaac
    14041 tggaaaacac tttttgcttt gtcagtaaca gttgttttct gatcttttaa gccagctgaa
    14101 gatgaggaaa gagcgtactg ctcatctgtc cattcaacat ttccttcgat ttttccttcg
    14161 ctgacaagtt ttccctcaaa gatggcggtg agccgctgaa catagtgaat gcttccgtta
    14221 ataaatccgt tgaatgttat tgtaaagccg ggttttgtat cgttatcgtg atcaaaaatt
    14281 ctggtgtcat tgtctttagt aggcattgac tctgttgaag gatcagccat gttggctcca
    14341 cgaagttccc attctttatt ggttttaaag aaaatatcat ctcccaccac gctggcttca
    14401 gcatcaagca tattttcctg acccgggatc tggttcggtt tccagaaatg gtaaattgtg
    14461 ttgaatttgg agttcggttc attgaaaccg acaactccct tattgagagc actgtctcct
    14521 gttctgttat cagttctgca catgataccg ttctttctgt caagagtaag gttgccgtcg
    14581 ttatcaagct ttatttctga aatgacatat cttgtcgtaa cgcttggaac atttttagcc
    14641 agcgatgtgc tggaaacaga tttaaggatt attttctgag cccatgttcc gatgaa
//
LOCUS       DAOOYB010000051        14591 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_4155_length_14591_cov_18.327600, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000051 DAOOYB010000000
VERSION     DAOOYB010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 14591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14591
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4155_length_14591_cov_18.327600"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="PL056_04655"
     CDS             <1..456
                     /locus_tag="PL056_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46336.1"
                     /translation="QLAEQLKASLAEAMKASDDEPEDTPDIEALLDKKLEEREMKAKT
                     KSEIDKWIEEHPDFKEPELGHKILDIIEKEKLPFNARTLQLAYDSITKDAQAKKATEE
                     ALKKEEVKNLERENASGVGGGSPTAKGQTPKDNPFDDLVGESINPNRVR"
     gene            513..1736
                     /locus_tag="PL056_04660"
     CDS             513..1736
                     /locus_tag="PL056_04660"
                     /inference="COORDINATES: protein motif:HMM:NF036552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 family protein"
                     /protein_id="HPM46337.1"
                     /translation="MPEPTHIIGTRNTLTIEQSRRIPDVDDKIFLLEPGESPLTAFLT
                     QIGKIGDGGGKFKGMALQKRVVYNPEFTEYEDQYSGVWAQINNGAGYLAGDTALVVDN
                     PGGSIFTKYDLIKNTRTGEIMRVTAVNYSTHTITVTRGAGATAAAAINDNDWLLIIGP
                     AFEEGSKSGDSNTTKLVKVTNFTQIFKTKFGVTETENASKLYPSGNPGADLKYLRAKH
                     GIEQAKKIERAYWFGEKKEVTGPDGKPLRLTGGVLEAIISAGNVQDEASSSLTEAEFR
                     SFLQNYAFKFGSSEKYFFCGNTILGHLESFATGKLMLTPSDKTFGVEVRKYQSSFGTL
                     NIVRHPMFDNQYAGMGVVLDLSTLKHCPLNGRDTTLETNIQDNDADEEMDQYKTEVGL
                     QRVNFEKNALIKGVV"
     gene            1838..2194
                     /locus_tag="PL056_04665"
     CDS             1838..2194
                     /locus_tag="PL056_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46338.1"
                     /translation="MAKYNSHIKKLRIVLKPAMPVYEAGVKVGDQPGEYAQFEDGQFE
                     TQDEAVIEKLESLGTFKIDFWRVSEESSPTEDTTVDKDLAKMTKKELQSLAQEKNVEV
                     DGTETKERLIELLLNK"
     gene            2227..3006
                     /locus_tag="PL056_04670"
     CDS             2227..3006
                     /locus_tag="PL056_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46339.1"
                     /translation="MPIVSADLKEYKSSNANSDGGGISATEVVDNTDNNLFTDITGDE
                     ASVGGTEYRKVFRKNNHGSLSWQNVVSWLQSQPTNSALSFGFGVDHADDADGAQGNMS
                     AFSANAVVAVVSDGADTRQVTIVGEDASGNRQTETLTLNGTTEVVGSLTFSKLYGAYV
                     NSLSGSRSVTIRQGSGGTSRGTIGINKKVSFIWYGKRYSGGSLVNAEGGDMASKVAGL
                     KHGDISSAGNFGVWYRLTWPAGAGAVTATTTQVKSEGDTAA"
     gene            3022..4575
                     /locus_tag="PL056_04675"
     CDS             3022..4575
                     /locus_tag="PL056_04675"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HPM46340.1"
                     /translation="MAKTFLTKKNNAKSTITDNPLLSGATTLNVQTGDGSKFPDAPFH
                     ATLYASDPATSEIVKVTVKSTDQFTIERAKENTTAQEWPQGTRLELLVTAKLFDDLQS
                     RTEIIVATDGSGDYNCDGTDDQTEINEAISNLPAGGGIVRIKKGTYNLTGVVEILKSN
                     VVLEGEGSATLLRAANASNLGDIVRVGNGGTTSYQNVTIRKFMIDGNQANQTSGASSP
                     LVLWGTSSYKHSRITIENMWLTGARQDCLKVIAIEDSVIKNNFIYSNIGTAIGLFTTS
                     QYISITGNILTSNSYGVYDSACNYCTMTGNVLRSNGYGFYVSNGWRETISGNTIFTST
                     NYGVYLIGSQRVTVIGNNFYGDSWSIVIGNSSNITRYNVITGNVIAWPTSQGIALYYG
                     SGTTVSNVITGNHIFGAGGHGIYLYASSYNTITGNAIDGSSRSSNNTSHDIYLSNNGS
                     VYSTYNVISSNNLQATQTNKVAYHIRELGANDDYNLVIGNICKDGVTGQINLQGANSV
                     RGTNIPASG"
     gene            4582..5883
                     /locus_tag="PL056_04680"
     CDS             4582..5883
                     /locus_tag="PL056_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46341.1"
                     /translation="MFNAYQFNTAQLNSTATKPIAKIMNYVMDVFGLISRPVTYVYDQ
                     FVLVFNLMTYKADILNLIEKAEIYVLDVYATINKIISYVLDIKSLITKAVSYTLDIRN
                     LITKAVSYVWDVIGGALMGVIYKLDIRQLAVKQTSYLLDILLMLGKTLTYRFDSFLSA
                     IKQTIYKLDIRNLATKLQSYLIDIGGVVARIHNYKLDILNLAEKQISYLHDIRALIAK
                     YWTYNFDIRSLIAKTQRYILDVFNKIEKTVDYALDILLLAVRQTAYILDIRSLANKLT
                     RYLFDIKNLAGRISTYKLDIKTLIAKIENYLWDIRSLITKTETYVLDVLSKITKTWTY
                     AFDVLIGIEKQWSYVLDIRGLIAKAWTYCFRIWKVIEGVKIAVEFLSAKLSVLMLSAK
                     ETLGLTTAKPNIEVKTARQNQQMQTSKSDINLPSNKKDINL"
     gene            5880..6194
                     /locus_tag="PL056_04685"
     CDS             5880..6194
                     /locus_tag="PL056_04685"
                     /inference="COORDINATES: protein motif:HMM:NF022117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HPM46342.1"
                     /translation="MSIKIVQNDTRPPLEFSLTQDGSPVDLTGCTVKFYMKDATTGTI
                     KINGTACVITDATKGKCRYNWSASDTNTVATYLGEVEVTFPDGKIQTGYKQLSIIIRD
                     DI"
     gene            6200..6832
                     /locus_tag="PL056_04690"
     CDS             6200..6832
                     /locus_tag="PL056_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46343.1"
                     /translation="MTRGEIKTAIKNEVDDQTLDTSLLNTWIQRADEKVQSWRPPEDR
                     NQTFDYWDYLKTEQPFTTVANQEKYPVPENYRAFIELKIGTDKTPYKLIDFRDRDKFD
                     DHAVYMLGGYFYPVATPTTDGNAMRFVFVRISDDFASDNDEPEVEKLYHQAYVEWGKK
                     MYYNQQGDTELERQADNNFERVMLGKWRDQELARMASASDQAEIPRSSLV"
     gene            6774..8531
                     /locus_tag="PL056_04695"
     CDS             6774..8531
                     /locus_tag="PL056_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46344.1"
                     /translation="MPEWRRLLIKLKFQEAVSYKNTMALPKKIWKTSQFHKGISNLNV
                     EGSFWFSNGLEFDQYPPFMKVANKFFKESDSVSLPALDEPTYMCYFNYRYYCVNHDDG
                     KIFQNDYSSWVEVHDSTNIWGGSGLYADDDYMYYAGSTWVGRFDKTTWVDNWQSFNVN
                     TAFDDVPITKFLKFICFGNKNYLATWDTGASVWNPNKLILPTGYVIVWLAPLTDYLVI
                     SAHHPTFGSALFFWDGYSQTYNRVLQLTQTQSLAGVVDKNVLYFLTRDGWICRFNGSG
                     IEKLNRFPEMNHEDYISLKAGCMKVYQGTILIAKGSNTYDVSRRSQYCGIWCYNPATN
                     ALYFKHRVSSGAVNHVTPGVSVMYGLYVDNNTIRVMYGDGNKSIIDVNHETGTFRPYE
                     FGAYWISPLIDNEPYLRKRWKQFILNFLKPMPNSSFARFEIKYNIAEQSIRDQAKYVT
                     GGGSDYFTLSSIGSSIEVGDEVIVLAGPAAGQIRHIKSIDTVTKTVYLDETLYYVGTS
                     QGYLQAGDYVAITPFKRLGTIKGNENQGKVNKMLRFNARSKKIQFKVEVWSNSGYSGE
                     WDMGVKDVSAVFVPDRIIK"
     gene            8942..10288
                     /locus_tag="PL056_04700"
     CDS             8942..10288
                     /locus_tag="PL056_04700"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HPM46345.1"
                     /translation="MSQITIQKGQTLSGIAKQYGTTVNDLLKANPTIKNPNLIYAGSA
                     LNLPGAQATPVVAPKASVPSPSPTVTPSKPVTTTVAQNSSPGASGVPEKTTMYGTDVP
                     SSTSLLKQYRSELGLDTALKSMQDSQTKYLETLQNLPKKSDIYQQERTKKGIDTMEKN
                     LQSLDARLATMEESINSSEKDIRDRIVGSGGLVTESQTQRLVASEKQPLIDEYRKLLN
                     ERNRLATQISTADTSAKEYAGMQYEDSALPLTVAEKTLGFQKDQYGVLGDILSQVFGA
                     SEKDVANLIDSAKYGKEQEKKEAQDVIDRAIKLAQLANETPAGTVLDIAGTKIVGTKG
                     SGSGSSSGGTSDSNDIEAYALELLNNPDFTVSNVPQSIRAKVIAKRDQIKAEAEAGST
                     MANSTPSMISSNNPPITAQQFGEGLKEVFTKQIPGVAKNVWGGTKGFFKGLFGVGN"
     gene            10295..13483
                     /locus_tag="PL056_04705"
     CDS             10295..13483
                     /locus_tag="PL056_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46346.1"
                     /translation="MAYIKDPKTGKYIKTDSMPTATVKTSVDTSAGQVKYEKNSQGKY
                     EVKPVDTSATTSAKPNVTTASGLRDFALQNGVDISETQPKESTLQKILNVLNTGAYAV
                     GGLISGKGVKQGIKEHVLPSEALGIKSKVGGFIADILLDPTTYITFGYGGGAKLATKA
                     GSVVLNKTGTKLLKKSITELGEGAARKAIAEKVLQEGGEKFLAKGGLKFAGQEILPRS
                     IATAPFKGADKLLEKTPMVGKLYQSAKDLAGKAFVPFKEIKDLPGGIGEQYVDKFSKF
                     SKATRYEVGKAVDEVSALGKTARKELGDKAGIEIGRTLENIPTPPVPIPRGLESVSHM
                     LKGVDLGDIKGVDRANIVDMKVFGSSVEGKLKPNDVDIFVKVKDGSMKFKNRGGLPSP
                     IVVKKGKIQYFIMPESQGDELLDAMLYTGRKDQSRLYSGATVKVPKNYWSGALKIADD
                     SINQPGSRKAVDDIVDFIRGEHKKFATAEKQRGLLDYELPDYLRHYLTPEGREFLQKS
                     GMNITSEINKPLRVKNPFAKTRQIDDTVEGINNYFRKRYGVRLFEQDAFKAFAARKAE
                     HIKAVGTYDFLTEIGKEFGKQAEVITKQIKNPVTGKMTAKEVIKPIFENGIKYIESSV
                     PQLKGTLLPEQIVKHVDDTYKTLTNEEATRRFLKLYDKVLGFWKGSVTGWFPAFHTRN
                     FMGGTFNNWIAGVTNPARYLQADQIARGSKGTITTKLGTKYGYEQIKELASKLGVVGQ
                     PGYLDVMKEVEKDVTKGPVAKLMGTPAKAMEVVENRLRLPLFVDRLIKGDAPEQAAKT
                     VFQFHFDYAPEALAPFEQNIMKRLLPFYRWTRGNIPLQLEQMVKQPGKYSAIGKGLQN
                     LQSDKEKAQEEFASLPPYMREGLPVRLGEKDGFSQYIYGFGLPVEDINRLYKGSAKRT
                     VASMVGELSPLLKYPIEVGTGQNLFTGEPIAESNRVYPFINKVPGLREWLEVREKENK
                     NGSVSYTANAYKLHFLNTALGRFYTTAGKLTDDKTSGAVKFLYGLLGSKAKSVDIEKE
                     KYWRDQELQDRLEQALESRGLIRQFERAYVPK"
     gene            13693..14205
                     /locus_tag="PL056_04710"
     CDS             13693..14205
                     /locus_tag="PL056_04710"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HPM46347.1"
                     /translation="MELKPDKIKYLVIHHSATARDTTTFDAVKRYHISKGWGDIGYHF
                     FITTSGKVMAGRSENTIGAHCVADSMNFKSLGICLPGNFMTEVPTVEQLSSLLDLLKK
                     LMSKYSIPKENVLGHREVSGASTACPGDNLQKWVSNFRGQKSEIDRSVIAEQVMNHLE
                     QAIDKLKQIL"
BASE COUNT         4728 a         3165 c         3321 g         3377 t
ORIGIN      
        1 caactggccg aacagctgaa agcaagtctt gccgaagcga tgaaagccag cgatgacgag
       61 ccggaggaca cgcccgatat tgaagcactt ctcgataaga agctcgagga acgagagatg
      121 aaagccaaga cgaaatccga aatcgacaaa tggatcgagg aacacccgga cttcaaagag
      181 ccggaacttg gacacaagat tctcgacatc atcgagaaag aaaaactccc gttcaatgct
      241 cggacactgc aattggccta tgactccata accaaggatg cccaagccaa gaaagcgacc
      301 gaggaagctt tgaaaaaaga ggaagtgaaa aatctcgagc gagaaaacgc atcaggtgtc
      361 ggaggcggtt caccaactgc caaaggccaa acgcccaaag ataacccctt tgacgatctt
      421 gtgggcgaaa gtatcaaccc taaccgagtc aggtaacgct tataagattt gaggtctcgt
      481 gagcggtatc gactcaataa aataacaaac ttatgcctga accaactcat atcatcggca
      541 cgagaaatac gctgacaata gaacagtcac gccgtattcc cgatgttgat gacaaaattt
      601 tcctccttga accaggcgaa tcaccgctca cagctttctt gactcaaatc ggaaagatcg
      661 gcgacggcgg aggcaagttc aagggcatgg ctctgcaaaa aagagtcgtg tacaacccgg
      721 aatttactga atacgaggat cagtactccg gtgtctgggc tcagatcaat aacggtgccg
      781 gttatttagc tggcgataca gcattggtcg tagacaaccc gggcggatcg atattcacca
      841 aatacgatct gatcaaaaac actcgtaccg gtgaaatcat gcgtgtgact gcggtcaatt
      901 actccactca caccatcacc gtaactcgtg gagccggtgc cacggcggcc gccgctatca
      961 acgataacga ctggctgttg atcatcggtc ctgcttttga ggaaggatcg aagtctggcg
     1021 attccaatac gactaaactc gtgaaggtca ccaacttcac ccaaatcttc aaaaccaagt
     1081 tcggtgtcac cgaaaccgag aacgcttcga aactatatcc gtccggaaat cccggtgcgg
     1141 acttaaagta cttgcgtgcc aaacatggta ttgagcaagc caagaaaatc gaacgcgcgt
     1201 attggttcgg tgaaaagaaa gaagtcaccg gtcccgatgg taaacctctc cgtttaaccg
     1261 gaggtgtttt ggaagccatc atctctgcgg gcaatgtgca agacgaagca agctccagct
     1321 taaccgaagc ggaattccgt tccttcttgc agaattatgc ctttaagttc ggctcgtctg
     1381 aaaaatactt cttctgcgga aacaccattc tcggacattt ggaaagcttt gccactggca
     1441 agcttatgct gactccgagc gacaagacat tcggtgttga agttaggaag tatcagtctt
     1501 cattcggaac gctgaacatt gtccgccacc cgatgttcga caatcagtat gccggtatgg
     1561 gagtcgtgct tgatttatca actttgaagc attgtccttt gaatggtcga gacaccacgc
     1621 tcgaaaccaa cattcaagac aacgatgctg acgaggaaat ggatcagtac aaaactgaag
     1681 tcggtctaca gagagtcaac ttcgagaaaa acgctttgat caagggcgtg gtctaaacct
     1741 tgatcatcgg ctctgctctg actcgctggc tttcaatttt ggaagccagg agtaagggcg
     1801 gaagccatca attaaaaact ttaaccaacc aacttatatg gctaaataca attctcacat
     1861 caaaaaactg cgaatcgtgc tcaagccggc catgccggta tatgaagccg gagtcaaagt
     1921 cggcgatcag ccgggcgaat atgcccaatt cgaggacggc caattcgaaa cccaagacga
     1981 agccgtcatt gaaaaacttg aatcgcttgg aacattcaag atcgacttct ggagggtctc
     2041 cgaggaatcg tccccgaccg aggacaccac ggtggataaa gacttggcca aaatgaccaa
     2101 gaaagaactc cagtctctgg cacaagaaaa aaatgtcgag gtcgacggca ccgaaactaa
     2161 agagagattg attgagctct tgcttaataa ataaagaccc aagaggtcga taatcaaatc
     2221 aaacttatgc caatagtatc tgcggactta aaagaatata agtcatcaaa cgccaattcg
     2281 gacggcggtg gaatttccgc cactgaagtc gtggataata ccgacaataa cttattcacc
     2341 gatatcaccg gtgatgaagc atctgtcggt ggaacggaat atcgaaaagt attccgcaaa
     2401 aataatcacg gctcgctgtc ttggcaaaat gtggtctcgt ggcttcaaag ccagccgact
     2461 aattccgcac tgtcattcgg atttggagtg gatcacgccg atgatgccga tggtgctcaa
     2521 ggaaacatgt cggcattttc cgccaacgcc gtagtggccg tagtttccga cggtgccgac
     2581 accagacaag tgacgattgt cggcgaggat gcatcaggaa acaggcaaac ggaaactttg
     2641 acacttaacg gcacaaccga agtcgttggg tctctcacat tctccaagct ttacggtgcc
     2701 tatgtgaact cattgtccgg atctcgttcg gtaacgataa gacagggatc aggaggaact
     2761 tcaagaggca caatcggaat caacaaaaag gtcagcttta tctggtatgg caaacgctat
     2821 tccggaggtt ctctcgtgaa tgccgagggt ggcgatatgg ccagcaaagt ggccggatta
     2881 aaacacggcg acatctcttc agccggcaat ttcggcgtgt ggtatcgcct aacatggcca
     2941 gccggtgctg gtgcggtaac agcgacaacc acacaagtaa aatccgaggg cgacacggcg
     3001 gcctaaaaga agttataagc tatggctaaa acattcctaa caaaaaagaa taatgccaaa
     3061 agcacaatca ctgataatcc actcctttcg ggtgcgacaa ctttgaatgt acaaactggt
     3121 gacggctcaa aatttcctga tgcaccgttc catgcaactt tgtatgcgag cgatcctgca
     3181 accagtgaaa ttgtgaaagt gacagtgaaa tcaaccgacc aattcacaat tgaaagagct
     3241 aaagaaaaca ccacggcaca ggaatggcct caaggcacac gccttgaact tttagtcaca
     3301 gccaaacttt ttgacgactt acaatcaaga acagagatta tagtcgccac tgacggttcg
     3361 ggtgattaca actgcgacgg tacagatgat caaacagaaa taaacgaagc aatcagcaat
     3421 ctgcctgccg gaggcggaat cgtgcgaatt aaaaaaggaa cttataacct gaccggtgtc
     3481 gttgaaatct taaaaagcaa tgtcgtttta gagggcgagg gttcggcaac gctcctgaga
     3541 gcggccaacg caagcaactt gggagatata gtgagagtcg gaaacggagg cacaaccagt
     3601 tatcaaaatg tgacgatccg aaaattcatg attgacggca atcaagccaa tcagacttca
     3661 ggtgccagca gtccgctggt gctatggggt acttcttcct acaaacacag ccgaatcaca
     3721 attgagaaca tgtggctcac cggtgcgaga caagattgtc tgaaagtcat tgccatcgag
     3781 gacagcgtga taaagaataa ttttatttat tccaacatag gcacggctat cggattattc
     3841 accacttctc aatacatcag catcaccggg aatattctga catccaattc ctacggcgtg
     3901 tacgacagtg cctgcaatta ttgcacgatg actggtaatg tcttgcgaag caacggctac
     3961 ggtttctatg tctcaaacgg ctggcgagaa actatatcgg gcaatacgat attcaccagt
     4021 actaactatg gggtgtattt gattggatct caaagagtaa ccgtaatagg caacaatttc
     4081 tacggtgaca gctggagtat tgtcattggc aacagctcaa acatcacccg atacaatgtc
     4141 atcactggaa atgtgattgc atggccaacc agtcaaggca tcgctttgta ttacggatca
     4201 ggcacgaccg tgagtaatgt gatcaccgga aatcatatct tcggggccgg tggacacgga
     4261 atatacctct acgcctcctc atacaacacg atcaccggaa atgccattga tggttcgtcc
     4321 cgatcatcta acaacacatc tcatgacatc tacctctcaa acaacggatc tgtttattcc
     4381 acttacaatg tgatttcctc caacaatctg caagcgactc aaacaaataa agtcgcttat
     4441 cacatcagag agctgggtgc gaacgatgac tataacttgg tcattggcaa tatctgcaaa
     4501 gacggcgtga ctggacaaat aaacctacaa ggagctaatt cggtgcgagg tactaacatc
     4561 ccagcttcgg gttaaacgat tatgttcaac gcttatcaat tcaacacagc acagcttaat
     4621 agcacggcga ccaaaccaat cgctaaaata atgaattatg tgatggatgt tttcggactg
     4681 atatccagac cggtcactta tgtctacgat cagtttgtct tagttttcaa tctgatgact
     4741 tataaagcag acatcctaaa tctgatagaa aaagctgaaa tttatgtgct ggatgtttac
     4801 gcaacgataa ataaaataat ctcatatgtg ctggacatca aaagtctgat tacaaaagcc
     4861 gtatcttaca cattagatat tcggaatctg ataaccaaag cagtctcgta tgtgtgggat
     4921 gtaatcggcg gtgcgttaat gggagtgatt tataagctgg atatcagaca actggccgta
     4981 aagcaaacga gctatctgct ggacatctta cttatgctcg gaaaaactct gacataccgc
     5041 tttgactcgt tcctttcggc gattaaacag acaatctaca aactcgatat cagaaatctt
     5101 gcaaccaaac ttcaatccta cctgatagac atcggcggtg tggtagcaag aattcataac
     5161 tacaaactcg atatcctgaa ccttgctgaa aaacaaataa gctatctgca cgatatcaga
     5221 gcattgatcg caaaatattg gacttacaat tttgacatca gaagcctgat tgccaaaaca
     5281 caaagataca tcctcgatgt tttcaacaag attgaaaaga ctgtcgacta tgcgttagat
     5341 attttgcttc tcgcagtaag acaaacagct tatattctcg acattcgaag tttggcaaat
     5401 aaactcacaa gatatttatt tgatatcaag aatttggccg gacgaataag cacatacaaa
     5461 ttggatatca aaacactcat tgccaagatt gaaaactatc tgtgggacat aagaagtttg
     5521 atcactaaga ccgaaaccta tgtgctggat gttttgagca aaataaccaa aacatggaca
     5581 tacgcattcg atgtcctgat cggaatcgaa aaacaatggt cgtatgtttt agatatccga
     5641 ggactaatcg caaaagcgtg gacttattgc ttccgcattt ggaaagtaat cgagggagta
     5701 aaaattgccg ttgaattttt atcagccaaa ttgtcggtcc tgatgctttc ggcaaaagaa
     5761 actctcggtc ttaccactgc caaaccaaac attgaggtca aaactgcacg gcagaatcag
     5821 cagatgcaaa catccaagag cgatatcaat ctgccaagca ataaaaaaga tataaaccta
     5881 tgagcataaa aattgtacaa aacgacacca gaccgccatt ggaattcagt ctgacacagg
     5941 acggctcgcc agtggatctc actggatgca cggtcaaatt ctatatgaaa gatgcgacaa
     6001 ccgggacgat aaagatcaac ggaactgcct gtgtgattac cgatgctacc aaaggcaaat
     6061 gccgatataa ctggtcggct tccgacacca acacggttgc gacttacttg ggagaggtcg
     6121 aggtcacttt tcccgacggc aaaatccaaa ctggatataa gcaattatca atcatcattc
     6181 gtgatgacat ttaaaaccta tgaccagagg cgaaataaaa acagcgataa agaacgaagt
     6241 cgatgaccaa acactcgata cctcattgct gaacacatgg atacaaagag cggatgaaaa
     6301 ggtgcagagc tggcgaccac cggaagatcg taatcaaact tttgattatt gggattactt
     6361 aaaaaccgaa caaccattca caacggtggc taatcaagag aaatatcccg tgccggaaaa
     6421 ttaccgtgcg tttattgaac ttaaaatcgg aacggataag acaccttata agctgatcga
     6481 cttccgggat agagacaagt ttgatgacca cgcagtgtat atgctggggg gttatttcta
     6541 tccggtggcg actccgacaa ccgacggcaa tgccatgaga tttgtctttg tccgaatatc
     6601 tgatgacttt gcatctgaca atgatgagcc ggaggtcgaa aaactctacc atcaggccta
     6661 tgtcgaatgg ggcaagaaaa tgtactacaa ccagcagggc gataccgagc tggaacggca
     6721 agccgataat aactttgaaa gagtgatgct cggaaaatgg cgagatcaag aacttgccag
     6781 aatggcgtcg gcttctgatc aagctgaaat tccaagaagc agtctcgtat aaaaacacta
     6841 tggcactacc taagaaaatt tggaaaacat cgcaattcca taaaggcatc tcaaacctta
     6901 atgtcgaagg gagcttttgg ttttccaacg gtttggaatt tgatcagtat ccgccgttta
     6961 tgaaagtagc taacaaattt ttcaaagaga gcgacagcgt gagtcttccg gcactcgatg
     7021 aaccgaccta catgtgctat ttcaattatc gatattattg cgtcaatcat gatgacggga
     7081 aaatttttca aaatgattat agtagctggg ttgaggtaca tgacagcaca aatatctggg
     7141 gaggaagcgg actctacgcc gatgacgact atatgtacta cgccgggagc acatgggtcg
     7201 gacgattcga taaaacgaca tgggtggaca attggcagtc gttcaatgtg aacacggcat
     7261 ttgatgatgt tccgattacc aagtttttga aattcatttg cttcggaaat aaaaactatc
     7321 ttgccacatg ggataccggt gcaagcgttt ggaatcctaa taagctcata ttgccgaccg
     7381 gttatgtgat cgtgtggctc gcaccactca ccgactatct cgttatttcc gctcaccatc
     7441 caacattcgg atcagctttg tttttctggg atggatattc tcaaacttac aatcgtgtct
     7501 tgcaacttac tcaaacccaa agccttgccg gtgtggtcga caaaaatgtc ttgtatttct
     7561 taacaagaga tggctggatt tgtcgattca atggttcggg aatagaaaaa ctgaaccgct
     7621 ttccggaaat gaaccacgag gactatatat cacttaaggc tggatgcatg aaagtatatc
     7681 aaggcacgat tctgatagcg aaaggatcga acacttacga tgtaagtcgc cgaagtcagt
     7741 attgcggaat atggtgctat aacccagcaa ccaatgctct gtatttcaaa caccgagtat
     7801 caagcggtgc ggtcaaccat gtgactcccg gagtatcagt catgtacgga ctttatgtgg
     7861 acaataacac gattcgggtg atgtatggcg acggcaacaa atcaatcatc gatgtaaatc
     7921 acgaaactgg aacattcaga ccttatgaat tcggggcata ttggatcagt ccattaatcg
     7981 acaatgagcc gtatctccgc aaaagatgga aacagtttat tctgaacttt ttaaagccga
     8041 tgccaaacag tagttttgcg aggtttgaaa tcaagtacaa catcgccgag cagagcataa
     8101 gagatcaagc aaagtatgtg accggtggcg gttcggatta tttcaccctt tcgtcaatag
     8161 gtagctcgat tgaagtcggc gatgaagtta ttgttcttgc cggccctgcg gccggacaga
     8221 tacgacatat taaaagcata gatacggtaa ccaaaacagt ctatctcgat gaaactttgt
     8281 actatgtcgg aacttctcag ggatacctgc aggctggaga ttatgtggca atcactcctt
     8341 ttaaaagact gggaacgatt aagggaaatg aaaaccaagg caaggttaac aaaatgcttc
     8401 ggttcaatgc gaggtcgaaa aagattcaat ttaaggttga agtttggagt aattccggat
     8461 actcgggcga atgggacatg ggagtgaaag atgtctcggc cgtctttgtg ccggacagaa
     8521 taatcaaata aagatatgag taaattcact ctggaaaaaa ttgatggcga gaatgtgaaa
     8581 aagatcgata cgcatgagtc ggtctcaatc gtaaacattc ctgctttgca aaaacaaaaa
     8641 gataatttag aaaaacaaat cgtcgtcatg caacaacagc tcgtccaaat caatgaggtc
     8701 ttggacgaat ataaaaaact tccatagata tgaacaatac accaaccaac aaagatcagc
     8761 cgtttgatcc gaatctgggt tcgatactca atttgctaag agacatacca gtactaaaca
     8821 caccgccgac cgatacgcca agaaccccga tcagcttcgc attatacgat aacggaagca
     8881 cacgacggtt ttatatcttt tttaatggag tgtggaggta cgcaacgcta acttaaaaat
     8941 tatgtcgcag ataacaatac aaaaaggtca gaccctttcg ggaatcgcca aacaatacgg
     9001 caccaccgta aacgatcttc tgaaagccaa cccgactatc aaaaatccga acctgatata
     9061 cgccggttct gccttaaatt tacccggagc acaggctaca cccgttgttg cccctaaggc
     9121 gagcgttccg agcccaagtc ccaccgtgac accttctaaa cccgtaacga ccactgttgc
     9181 ccaaaattcg agcccagggg cgagcggtgt acctgaaaag accacgatgt atggaactga
     9241 tgtgccttcc agcacttctt tgcttaaaca ataccggtcc gaattaggcc ttgataccgc
     9301 cctaaagagc atgcaggaca gccagactaa ataccttgaa accctgcaaa atcttccaaa
     9361 aaaatctgac atctaccaac aagaaagaac caagaaaggc attgatacga tggaaaagaa
     9421 tttgcaaagt ttggatgcaa gactagccac aatggaggaa tctatcaaca gctcagaaaa
     9481 agacattaga gacagaattg ttggaagtgg cggtctcgtt actgaaagcc aaacccaaag
     9541 attagtcgct tcggaaaagc aaccgcttat agatgagtac agaaaattgc tcaacgaaag
     9601 aaatcgactc gctacacaaa taagcactgc ggatacttcc gctaaagaat acgctggtat
     9661 gcaatatgaa gattcggctc ttcctctgac agtggccgag aaaactctcg gatttcaaaa
     9721 agatcaatac ggtgtgcttg gcgacatact aagccaagtg tttggtgctt cggaaaaaga
     9781 tgtcgctaat ttaatcgact ccgccaaata cggaaaagaa caggaaaaga aagaagctca
     9841 agatgtaatt gaccgggcga taaagcttgc ccaactcgcc aatgagactc cagccggaac
     9901 cgtgcttgat attgctggga caaaaattgt cggcaccaaa ggaagcggat caggttcatc
     9961 aagtggcggt acgagcgatt ccaacgatat agaagcttat gcgttggaac tattaaataa
    10021 tcccgacttc accgtaagca atgttccgca aagtattaga gcaaaagtaa tcgccaagag
    10081 agatcaaata aaagcggagg ctgaagctgg atcaaccatg gccaacagta cgccatcaat
    10141 gattagtagc aataatcctc cgattactgc tcagcaattt ggcgagggat tgaaagaggt
    10201 cttcactaaa cagataccgg gcgtggcaaa aaatgtgtgg ggaggaacca agggattctt
    10261 caaaggactc ttcggcgtag gaaattaaaa aactatggct tatatcaaag accctaaaac
    10321 tggcaaatac atcaagactg attccatgcc aacggcaacg gtaaaaactt ctgtcgacac
    10381 ttctgccgga caagtaaaat atgaaaagaa ttcgcaggga aaatatgaag taaagcccgt
    10441 tgacacttct gccacaactt ctgccaagcc taatgtgaca acagcctccg gacttagaga
    10501 ttttgcgttg caaaacggtg ttgatatcag cgaaactcaa ccgaaagaat caaccctgca
    10561 aaagatactc aatgtcttaa ataccggagc gtatgccgtg ggaggactta taagcggtaa
    10621 aggagtaaaa caaggaatta aagagcatgt tttgccgtcc gaagcattgg gaataaaaag
    10681 caaagtcggc ggatttatcg cagacatact gcttgatcca acgacctaca taacattcgg
    10741 ttatggtggc ggtgcaaagc tggcgaccaa agccggaagt gttgttttga ataagacagg
    10801 aaccaagcta ttaaaaaaat ccatcactga acttggcgag ggagcggctc gaaaagcaat
    10861 tgcggaaaaa gttttgcaag aaggaggcga aaagtttttg gccaagggcg gacttaagtt
    10921 tgccggacaa gaaatattgc ctcgatcaat agccaccgca ccattcaagg gagcggataa
    10981 attactggaa aaaaccccga tggtcgggaa gctttatcag tcggccaaag atttggccgg
    11041 aaaagctttt gtgccattca aagaaataaa agacttgcca ggaggaattg gcgaacagta
    11101 tgtcgataag ttttcaaaat tcagcaaagc gacaagatac gaagtcggca aagccgttga
    11161 tgaagtatcg gctcttggca aaacagcaag aaaagaactc ggcgataaag ccggaatcga
    11221 gatcggcaga acgctggaaa acatcccgac tcctccagta ccaatcccaa gaggccttga
    11281 atcagtaagt catatgttaa aaggcgttga cctcggcgac atcaagggtg tggatcgtgc
    11341 caatatagtc gatatgaaag tgttcgggtc atccgttgag ggcaagctta aaccgaacga
    11401 tgtggatatt tttgtgaaag tgaaagacgg ctcgatgaaa ttcaaaaatc gtggtggatt
    11461 accgtcgccg attgtggtca agaaaggaaa aatacaatat ttcataatgc cggaaagcca
    11521 aggcgatgaa cttctcgatg cgatgcttta taccgggcgt aaagatcaaa gtcgattata
    11581 ttccggtgcg accgtaaaag taccaaagaa ttattggagt ggtgctttga aaattgccga
    11641 tgattcgata aaccaacccg gaagtagaaa agcagtcgat gacatcgttg actttattcg
    11701 gggcgaacat aaaaaatttg ccactgctga aaaacagcgt ggtcttttgg attatgaatt
    11761 gcccgattac ctaaggcact acttaactcc cgaagggcgt gaatttttac aaaagagcgg
    11821 gatgaacatt acttccgaaa taaacaaacc gcttcgtgta aaaaatccat ttgccaagac
    11881 aagacagatt gatgacacag tcgaaggaat aaacaactac ttccgcaaaa gatatggcgt
    11941 aagacttttt gaacaggatg cgttcaaagc attcgcggct cgaaaggccg aacacatcaa
    12001 ggctgttggc acctacgact ttttgaccga aatcggaaaa gaattcggca agcaagccga
    12061 agtgataacc aaacagatta agaatccagt aactggaaag atgaccgcca aagaagtaat
    12121 aaaaccaata ttcgaaaatg gcattaaata tattgaaagc tccgtgcctc aattaaaagg
    12181 tacattgttg cccgaacaga tcgtgaagca tgtcgatgac acttataaga ctttgactaa
    12241 cgaggaagca acaaggagat ttttgaagct ctacgataag gttctcggtt tttggaaagg
    12301 atcggtcact ggatggtttc ctgctttcca cacaagaaac ttcatgggag gaactttcaa
    12361 caactggatt gccggcgtga ccaatcctgc cagatatttg caagctgatc agatagccag
    12421 aggatcaaaa ggaacaatta ccaccaagct cgggactaaa tacggatacg agcaaataaa
    12481 agaactggca agtaaattag gagtcgtcgg tcagccggga tatttggatg tcatgaaaga
    12541 agtggaaaaa gatgtcacta aagggccggt ggccaagctt atgggaacac ctgcaaaagc
    12601 aatggaagtc gtggaaaaca gactgcgact gccactgttt gttgatcgat taattaaagg
    12661 cgatgcaccc gaacaggccg ccaaaacagt cttccaattt cacttcgact atgcaccgga
    12721 agccctagca ccatttgagc aaaacatcat gaagcggtta ttgccgtttt acagatggac
    12781 tcgtggaaat attcctctgc aacttgaaca aatggtcaaa cagccaggca aatattcagc
    12841 catcggaaaa ggattgcaga acctacaatc agacaaagag aaagctcaag aggaattcgc
    12901 ctcattgcct ccttatatgc gagaggggtt gccggtccga cttggcgaaa aagacggctt
    12961 ttcgcaatac atctacggct tcggtttgcc cgttgaggat ataaaccgac tttataaagg
    13021 aagtgcaaaa agaactgtgg ccagcatggt tggcgaatta tctccgcttt tgaaataccc
    13081 gattgaagtg ggtactggac aaaatctgtt cactggggag ccaatagccg aatcaaaccg
    13141 ggtgtatccg tttataaaca aagtaccggg gctgagagaa tggctggaag ttagagaaaa
    13201 ggaaaataag aatggttcgg taagttacac cgccaatgct tacaagcttc actttttaaa
    13261 cactgcattg gggaggttct ataccacggc cggaaaacta actgacgata aaacatcggg
    13321 tgcggttaag tttctttatg gattactcgg ctcaaaagca aaatcagtgg atatagaaaa
    13381 agaaaagtat tggcgagatc aagaactgca agaccggctt gaacaggcgt tggaaagtag
    13441 aggtctgatc agacaatttg aaagagcata tgtaccaaaa taaaaatatg aatgacgaat
    13501 cttatcaaaa caacatgcag gacaaacggc ttgatagtat cgaaaagcat attgtcatta
    13561 ttaacgagga aatggggtcg gttaaaaatg acatggcttg gatcaagaag tttttctggc
    13621 ttatcgccac tgcatcagtc ggcggactta taaccgcatt aatcaattta ttaatctcat
    13681 tagcaaaaca ctatggaact taaacctgac aaaatcaaat atttagttat ccatcattct
    13741 gcgaccgcca gagataccac gacattcgat gcggtaaaaa gatatcacat ctcaaaaggc
    13801 tggggcgaca tcgggtacca cttttttata acaaccagcg gtaaagtgat ggctggacga
    13861 tctgaaaata cgattggggc acattgtgta gccgacagca tgaatttcaa atcgcttgga
    13921 atttgtctgc ccggaaactt catgactgaa gtaccgaccg ttgaacaatt atcctctttg
    13981 ttggatttac tcaaaaagct gatgtcgaag tacagcattc cgaaagagaa tgttctcggc
    14041 catcgtgagg tcagcggagc ttcgacagct tgcccaggag ataatctcca aaaatgggta
    14101 tctaatttta gaggccaaaa gagcgaaatt gaccggtctg tgattgccga acaggtcatg
    14161 aaccatttag aacaagcaat cgataaatta aaacaaatac tatgacacaa gcacataaag
    14221 aggccttaaa gcatcttgcg atagttttcc tatattccgg agtctcgtcc attctgccga
    14281 tcctgttggc ttatgtacag aatgaccctc gttgggcatt gttaataccg cttataaact
    14341 cagcgtggta tgccgtaacc cgatacctga aagaaaggaa actgatcgag gaagccctaa
    14401 cgagaggcga tgaattttag ctaaattaag caattcagat taatagactt tttgagcaaa
    14461 agccggaaca tcaaaatagc taggattatc cttgtaaatt cggcgtaatt ggcgtataat
    14521 tttatcaata cgactcattg accagcttct ggtggtgtaa actggttcaa agttccggaa
    14581 atcgccaccg c
//
LOCUS       DAOOYB010000052        13792 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_4505_length_13792_cov_12.508554, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000052 DAOOYB010000000
VERSION     DAOOYB010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13792
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4505_length_13792_cov_12.508554"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            180..3914
                     /gene="dnaE"
                     /locus_tag="PL056_04715"
     CDS             180..3914
                     /gene="dnaE"
                     /locus_tag="PL056_04715"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HPM46348.1"
                     /translation="MAFVHLHLHTIYSFLDGLVKPSELLDNLKKLGMKAAAITDHGNM
                     HGAIDFYTTMIGKATSLKEADIKPIIGMEAYISSGDRTEKTKQDAYHIILLAKDNEGY
                     RNLCYIASTAFIDGFYYKPRIDRALLKDHAKGLIAFSACLGGEIPKALLSGRYSDAKK
                     IALEYQELFGKDDFYIEIQVNGLKEQEEVNPLLIKLAREIGAGLVATNDVHYLKPENA
                     VAQDILFCINEKKKVEDEDRMHHESNAFYLKSEDEMRHLFSVYGEAGAEAIENTWKIA
                     EKCNVELKLRVPYLPDFETHGMTEPEYLSNLAGKGLEQRFTELGTPEEKKKEYYDRLE
                     YELKMIEKTGFAGYFLIVSDFIGWAKDNDIPVGPGRGSGAGSLVAYSTRITDLDPLRF
                     GLVFERFLNPERISMPDFDVDFCQDKRDQVIDYVSKKYGEKNVAQIATFGQLKAKSAV
                     RDVARALNISLSTANDLAKLVPDSFSELVTPQFSKKTKDITRKLIDEYGVSSETANDP
                     DLLRTTLEKLPLRQDDIAAVKLLISEIDTYVNERILIKNNRDYKRIVDIATQIEGLLR
                     QPGKHACGLVIGQKPIFEYSPLFVDKDNYRVTQYDKNMVELVGLVKFDFLGLKTLTMI
                     NHAVTLVRRKIPGFDINKIPFDDAPTYRFLCDKSTQGIFQMESAGFEKMIHQMKPDRI
                     EDLIAGVALYRPGPMDIIPNYIRRKHGREPIEYEHEWLEEILKETFGLIVYQEQVMQI
                     SRKMAGFTMGKADTLRKAMGKKLAKEMEKMKSEFIEGAQKLSVDKDVAEQVFDHMEKF
                     ASYGFNKSHAACYGYISYQTAYLKTHYPVEFFTALISSESASADKVFSYISDARSMGV
                     SVYAPDINKSDFSFSIEENSIRFGLGAIKNVGEGAIEVIIDERKKNGPFKSLIDFTAR
                     INQSKVNSRTIEFLIKSGAFDFTGINRGLLLSMLPYAIKEGEKSAADRESGQVSLFDA
                     FSIAGEGCKVCDNDETILNMVENRQELDIFDSLALEKEVLGIYLSNHPANLFVKDFLG
                     LGIASIKEIVDEVESGRRFYGQRTEAVWVPGIITTDIKPAKGREGEYYLKGVIESSDA
                     AAEFTINRIDNPLANPAVAKLDSKVPLMFKVKVRALRNRDDNTLEKVVLNIEDIGRDV
                     LTIDEFLFEQRKNNEGFRIRMVAEISEKEFLSRKKEISSMLFNRNVPGTEMAFVIKIF
                     FDEGKNHALLEGKANVNIERIPEYKKVFGYNTLYLAGRLK"
     gene            3911..4846
                     /locus_tag="PL056_04720"
     CDS             3911..4846
                     /locus_tag="PL056_04720"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HPM46349.1"
                     /translation="MKKLLMFSLFFFVNTFLFSQTVTFKYSEDRFEALYKTVSKHYKK
                     DELKQLLEKSEVKEIDPATEKNLKFILNPRSVKQQASKHEDYISKLVNDETVKKGVDF
                     FGKYNAVLEEVYKKTKVHPADIIAIINWESKLGEMTGDQQIIKIFIGQYFFAEDYNAM
                     LIKEGAYEKEGAMTREQADKRIERLKKNALGNLTALLTQAKLRNFDPATVKGSWAGAI
                     GYPQFMPASMCFAADGNNDGKIDLFTMEDAIASVANYLKENGYHSKGREHSFKRYNPD
                     RIYAKGVKMYGDMARKAGVEPGKCPPSGAACPIDL"
     gene            complement(4843..7125)
                     /locus_tag="PL056_04725"
     CDS             complement(4843..7125)
                     /locus_tag="PL056_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46350.1"
                     /translation="MKKLLLAATIIILFANLVSAKEMKTEFFNINRTVRTSADLKNFV
                     KLELPSVISLKNSSIKGELLGTNEISGMKIHKIQWSYNGIPVVGRYTVINEKGGKVLN
                     VINGLKDFSLNTAPDLSSVEAAEKVSFQNNGNIVADHDFISNLVIIERFGKYRLAYRI
                     RFKPSSILDGRFYYVDAHDGKYLGGGNFVMKSSENTAKVFNTNPIRDKEAVEVELPWV
                     ADDAEGKLTAEIDDAGIRKVVASNCLDLGDTMDYYGSKYPICTPTQNASKIDNGNFIY
                     EDWNKGVAYSFDVEDNYSEIAVYWHMTRVYKYLIDLGLKNFTHLATHNKAGQLNPIIG
                     VGNFQMPESTTRLAPMDNAFYSPHDPYFKDMFFQNFVYEGDIIVLGQGSKADFAYDGD
                     VIYHEFGHATIEGTAKLSYMAFPDKYGYSNETLGINEGMADTFSFIISGDPCLGEYVS
                     EAYGSRYGYEKTGDYYCLRHAENEEIVNESFTGESHHDGLPAVSAHWLMYQHALEKGF
                     NMDDFAKYFMTSLLSIVFSDLDYEGWGNILLKTVKNTDLAPLEETFRKILEDKGFFSE
                     IRARNIMSKVDYLFSGGIAEYQGMPSSTLAVEIEGNLIEVAPMYVQLYYDVPECVDTL
                     TITGMATDGQSMSTSSAPKYNLLARKDEPVIWTVDSEPLKVDYDTYIKGDGEWVAKGL
                     ESGKRYYFQFINTGPEGLLYNPKAVGSWSSENECNAEVTDEEVSDNEVNDEEIDDTEL
                     PDDQINPEKKDDSGCSLSVI"
     gene            complement(7222..10479)
                     /locus_tag="PL056_04730"
     CDS             complement(7222..10479)
                     /locus_tag="PL056_04730"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HPM46351.1"
                     /translation="MNGFSAKDVPLKGMNLIEASAGTGKTYSIAAIFLRLVLEGAPVE
                     SILAVTFTKSATAELKNRLLDFLRSAKKILEGTDKTQNGTLIGQIASQYSDNREESLK
                     KLRRAITNFDRSSVFTIHGFCSRIVRENAFESGARFNLELSKENSEPVEQAVIDFWRK
                     YSGYMDEEFINRASKEKWFSVDELIKLIKGRTLCEKPLIKPAVERTELSFERNREITS
                     DPHALEKEFCTDALILVDRLFDEAKKNIIDRRDRTGKMSFDDLLSILESALSRNDAFS
                     KQLVAIMEKKYHAVLIDEFQDTDPVQYSIFTKLFGTSGNPVFYIGDPKQSIYSFRNAD
                     VFAYLKVSSSQKSITKYSMEKNFRSSPMMTEAVNALFSSGKNPFIIDESEIGYKPVKP
                     KDADCRNDLKINDIPHHGLEIRYLSALDPKYSAFTTGSRKFPEKQKIKLAPVKGIVFD
                     DLCSKILQLVDENNGYTIEGVPVRPGDITILVIKHSEATEIKSYFERSGIPAVLTSDQ
                     SVFMSKEMQDLLIIMEAAANPIPSRIKAALLTMFFNMGPSEIIDIADDPPVFEHYLDL
                     FSNIYRNWEEKGFLTAFSEFMNSENRYSLLAGYGERTLTNIRHLFELLHNRESVAGRS
                     VEPLLSWVKEKMTGDEKNEDEQLRLESDENAVSIMTVHKSKGLDFKIVFIPYFIKKPG
                     TKKSWYFHYHDKADNYRPVLSFDINDKAAADLSRIETMSENLRLLYVAMTRAKFLTVI
                     YWGNINSTGSAPLSKILFNVCDDDVFKNMTDSELMKKVNDLEEKSGGAIKVDTDLSGN
                     GKALKKEYGKTPLIPARKMKKGINMEWAVTSFSAISSSKQSEHISNVISHSEEAVRSD
                     ISLFPAGASAGTVLHSIFENIDFSSGDNSDVISSILDSNYMRYHSSEADMLPWVERCV
                     ENVLNAPVFDGSSLKNVKKNSIASELEFFLPSKKFAYREISDILGDAVNIEGGSISGF
                     IHGFIDLVFRHNGKYYIVDWKSNKLGNDYSCYDDDEIKKEMVRHNYVLQYMLYLAAFD
                     TYMSKTDENYSYESDFGGIRYIFLRGVTPENGYVTGIYKDLPHIDTVRKLQSLFR"
     gene            complement(10476..13715)
                     /gene="recC"
                     /locus_tag="PL056_04735"
     CDS             complement(10476..13715)
                     /gene="recC"
                     /locus_tag="PL056_04735"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HPM46352.1"
                     /translation="MPSSSGFFLYQSNMLEMLVEKAVPIINGLCYKDPLKKNMAVVQT
                     SGMGRWLSLRIAERSGICANIETILPENFIQHIASIIFDEKGSDPFFETPNLRWFIYD
                     LLSTEFIDKKELKPVRTYIGKDDVKKYQISGHIADMFDQYQIFRPDIIAKWKKGETVY
                     KKDKDEIWQKEIYSAIISAKPDSLDRAAFFDAFRNKIEKRDLSKLPLPEGVVLFGIST
                     MTAFQLELFKSLSKIMEVHLFHMDPSREFWGNIETTEFSGKDSFFYESDNPLLSELGF
                     AGKNFFNLLSESDGQHIDCFAPDTEGKTILNRLQNDIKACVNPKPCHLDFDGSVIING
                     CWGKMREMEVLKDTLLSLFDSNEDLNPRDVIVMAPQIEEYSDFIEAVFGSGSSGNLIP
                     FTVADRSATKESGLIDPFLNILSLDGTNFSRTAVIAIFENPNIYRKFNVEKEDLESIK
                     KAVSESGIKWGIDKNWRRSRGYHEFEQNSWKFGIDRMLLGYALPGKGTETFSDILPYD
                     DIEGGIARTIAKFISFTDLIFEFRNDFYESRTLAQWEEKLNRLIDLLFIDNAETSATL
                     RYLRDSFNRLVKSTAAGGFCGKVPLSVVREHLNDYFSNSGFGRGFMNGQVTFCSLKPM
                     RSIPFKVIALIGMNDESFPRENMSSAFDLMFKYPRITDRFVKENDKYLFLEALICARE
                     KLVISYIAKSMNDSSKNIPSSVVESLRNYITEKYCVKPDQIEKLHPLQPFCAKYFMEG
                     SNLFSYSERDFCAAKHFHSGSPEQVAGKVNGTVDVSRPELRQKTNMQKITVNDLLSFF
                     ANPQKYFFRNTLDIVFPYIRKEDDDLELFTFDNLQGYGLKNEFAKMNQAGFSAKDFMT
                     KMRGSGLIPHGSTGRVLLNRQIEELPAFLDKIKNLKGTDAEETVSVDLKFNIGKNEIS
                     LRGDLDNVFKDRQVLFRPSKTPKDKDRVKALILHLAMNASGVMKETCFSFMDADLFFK
                     PSQKSCEELSDLVEIYLNGMSKIPLFNPWIAEKLFSGNKPGRSEREIFNMMIEKMDDD
                     FGGMTRDIYFDMAARITGFFESNDETIAEVIGTARKIFSKISDLSEEIK"
BASE COUNT         3943 a         2849 c         2958 g         4042 t
ORIGIN      
        1 ctcaaaaaaa caaaccggtt cataataaaa cggcaggagg gaatgtcaag tcgaagaaca
       61 attttcccaa tgggcaaagt tatttctcaa tgattttaag taagcccttt tttgcctctt
      121 tactataact tttttattta tagaatagct taagaaaaaa acttttcccg ggggaatcca
      181 tggcttttgt tcaccttcat ctgcatacaa tttattcgtt cctggacggg cttgtcaagc
      241 cgtcggaact tcttgataac cttaaaaagc tcggcatgaa agccgctgcc atcacagatc
      301 acggcaatat gcacggagcg atagattttt atacaacaat gatcggtaaa gcgacatcgt
      361 tgaaagaggc cgacattaaa ccgataatcg gaatggaggc atacatctct tccggtgaca
      421 ggactgaaaa gaccaagcag gacgcatacc acataatact tcttgctaag gacaacgaag
      481 gttacaggaa tctctgctat atcgcatcaa cggctttcat cgacggattc tattacaagc
      541 cccgtatcga ccgcgcactt cttaaagatc acgcaaaagg gctcattgcg ttcagtgcct
      601 gcctcggagg tgagatcccc aaagcacttt tatcgggaag atacagtgat gcaaaaaaga
      661 tagcgcttga atatcaggaa ctttttggaa aagatgattt ctatattgaa atacaggtga
      721 acggactgaa ggaacaggaa gaggtaaacc cccttctgat aaagcttgcc agagagatcg
      781 gtgcaggact tgtggcaacc aacgatgtcc attatcttaa gccggaaaac gctgtcgctc
      841 aggacattct tttctgcatc aatgaaaaga agaaagttga ggacgaagac agaatgcacc
      901 acgaaagcaa cgctttttat cttaaaagtg aagatgagat gcgtcatctt ttcagtgttt
      961 acggtgaagc aggagctgaa gcgatagaga acacatggaa aatagctgaa aaatgtaatg
     1021 tggagctgaa gctcagagtt ccgtatcttc ccgattttga gacccatggt atgaccgagc
     1081 cggaatatct ttcaaacctt gccggaaagg gacttgagca gagatttacc gaacttggga
     1141 cacctgaaga aaaaaagaaa gagtattatg accggcttga atatgagctg aaaatgatag
     1201 aaaagacagg attcgccgga tatttcctca tagtttcaga ctttatcggc tgggctaagg
     1261 ataatgatat tcctgtcggc ccgggaagag gcagcggcgc aggttcgctt gttgcatatt
     1321 cgacaaggat aaccgatctt gatcctctcc ggttcgggct ggtttttgaa agattcttaa
     1381 atcccgaaag aatttcaatg cctgattttg atgttgattt ctgtcaggat aaaagagatc
     1441 aggtcattga ttatgtttca aaaaaatatg gtgaaaagaa tgtcgcccag atcgcaacat
     1501 tcggtcagtt aaaggcaaag tcagcagtca gggatgttgc aagagcactc aatatatcac
     1561 tttcaactgc aaacgatctt gccaagcttg ttcccgattc cttttcagaa ctggtaactc
     1621 cccagttctc aaaaaagaca aaggacatca caagaaaact gatcgatgaa tacggcgtct
     1681 cttctgaaac ggcaaacgac cctgatctgt tgagaaccac acttgaaaaa cttcctctca
     1741 ggcaggatga tatcgccgca gttaagcttc tcataagcga aatagacaca tatgtgaacg
     1801 aaaggatact tataaagaac aacagggatt ataaaaggat agtcgatatc gcaacgcaga
     1861 tagaaggact tttgagacag ccggggaaac atgcctgcgg actggtcata ggacagaaac
     1921 cgatatttga atattcgccg ctgtttgttg acaaggacaa ctaccgggtg acacagtatg
     1981 acaagaacat ggtggaactt gtaggtcttg tgaaattcga ctttctcgga ctgaagactc
     2041 ttacgatgat aaaccacgct gtaacccttg tcagaaggaa gattcccggt tttgacatca
     2101 acaagatccc gtttgacgat gcccccactt accggttcct ttgtgataaa agcactcagg
     2161 ggatattcca gatggaaagt gccggttttg aaaaaatgat ccatcagatg aaaccggata
     2221 gaattgaaga tctcatcgcc ggtgttgcgt tgtatcgacc gggcccgatg gatataattc
     2281 ccaactacat cagaagaaag cacggcaggg agccgatcga atatgaacac gaatggctgg
     2341 aagagatact gaaggaaact ttcggactca ttgtctatca ggaacaggta atgcagatct
     2401 cccgtaaaat ggccggattc acaatgggca aggcagatac cctcagaaag gcaatgggaa
     2461 agaagcttgc aaaggaaatg gagaagatga agagcgaatt catcgaaggg gcgcagaaac
     2521 tttccgttga caaggatgtg gcagaacagg tctttgatca catggaaaag tttgcaagct
     2581 acgggttcaa caagtcgcat gccgcctgtt acggttatat ctcatatcag accgcatatc
     2641 ttaaaaccca ttatccagtg gaatttttca cagcactaat ttcatctgaa tctgccagtg
     2701 cagacaaggt tttcagctat atcagcgacg caagatcaat gggtgtgagc gtctatgccc
     2761 cggatataaa caaaagcgat ttttcgttca gcattgagga aaattctata agattcgggc
     2821 tcggagcgat aaagaatgta ggtgaaggag ccattgaggt tattatcgat gagcgtaaaa
     2881 agaacggccc gttcaaatcg ctcattgatt tcacagcaag aataaatcag tcaaaagtca
     2941 attcaaggac gatagagttc ctgatcaaat ccggtgcgtt cgattttacc ggaataaaca
     3001 gaggacttct tctttcgatg cttccctatg cgataaagga aggtgaaaag tcagccgcag
     3061 acagggaatc aggacaggtg agtctttttg atgctttcag tattgcaggt gaagggtgca
     3121 aagtgtgtga caacgacgaa acaattctca atatggtgga aaacagacag gaacttgata
     3181 tttttgattc ccttgcactt gaaaaggaag tgctcggaat atatctttca aatcatcctg
     3241 caaacctttt tgtaaaggat tttctgggac tcggcattgc aagcataaaa gagatcgttg
     3301 acgaagttga atcgggcaga aggttctatg gacagagaac tgaagcggtt tgggttcccg
     3361 gtataatcac aacagacata aagccggcaa agggaagaga aggggaatac tatctcaaag
     3421 gtgtcatcga aagctctgat gcggcggctg aatttacaat aaacaggata gataatccgc
     3481 tcgcaaatcc ggcagtggca aaacttgatt caaaagttcc cctcatgttc aaagttaaag
     3541 tgagggcatt gagaaatcgc gatgacaata cgcttgaaaa agttgttctc aacattgaag
     3601 atatcggacg cgatgtgctt actatagatg aattcctgtt tgaacagaga aagaacaacg
     3661 aagggttcag aataagaatg gttgccgaga tctctgaaaa agagtttctg agcagaaaaa
     3721 aagagataag ttcaatgctt tttaacagaa atgttcccgg aacggaaatg gctttcgtga
     3781 taaaaatatt ctttgacgaa ggaaaaaacc atgcactcct tgaagggaaa gccaatgtaa
     3841 acattgaacg aatccctgaa tataagaagg ttttcggtta taacactctt tatcttgcgg
     3901 ggagattgaa atgaaaaaac ttctgatgtt ttctctgttt tttttcgtga atacttttct
     3961 tttttctcaa accgtgacat tcaaatacag cgaagacaga tttgaggcgc tgtataaaac
     4021 agtttcaaaa cactataaaa aggatgaact gaaacagctt cttgaaaaga gcgaagtaaa
     4081 agagatcgat cctgcgacag aaaagaatct caaattcata ctgaatccgc ggtctgtaaa
     4141 acagcaggcg agtaagcatg aagactatat ttccaaactt gtaaatgatg aaacggtaaa
     4201 gaaaggagtt gattttttcg ggaaatacaa tgccgtttta gaagaagtct ataaaaagac
     4261 caaagtccat cccgccgaca taatcgcaat aatcaactgg gagagcaaac ttggagagat
     4321 gacaggtgac cagcagataa taaagatatt catcggacag tatttctttg cagaggatta
     4381 taatgcgatg ctgataaagg aaggggcata cgaaaaggaa ggggcgatga cccgtgagca
     4441 ggcggataaa aggatagagc ggcttaaaaa gaacgcgttg ggaaatctca cagctcttct
     4501 tacacaggca aaactcagga attttgatcc ggcaactgta aaaggttcat gggcaggtgc
     4561 gattggatac ccacagttca tgccggcatc aatgtgtttt gccgctgacg gaaataacga
     4621 cgggaagatc gatcttttta caatggaaga tgccattgcc tctgttgcaa attatcttaa
     4681 agagaacggt tatcattcaa aagggagaga acactcattc aaacgataca accctgacag
     4741 gatctatgca aaaggggtga agatgtatgg cgatatggca aggaaggcgg gagttgagcc
     4801 ggggaaatgt cctccgtcag gtgccgcctg cccgatagat ctttaaatta cagagagcga
     4861 gcatcccgaa tcatctttct tttcaggatt tatctggtca tcaggaagtt cagtatcatc
     4921 aatttcctca tcattaactt cattgtcgga cacctcttca tctgtgactt cggcattgca
     4981 ttcattttcc gaactccatg aaccgacagc tttcggattg tagagaagtc cttcggggcc
     5041 tgtgtttatg aactggaaat aatatctttt tccgctttca agtccttttg caacccattc
     5101 tccatcgcct ttaatatagg tgtcgtaatc aacttttaaa ggttctgaat ccacagtcca
     5161 gattaccggt tcatcttttc ttgcaagcag attgtattta ggagcagaag aagtgctcat
     5221 tgactgtccg tcggtcgcca tccctgttat tgtaagagtg tcaacacatt caggaacatc
     5281 gtaataaagc tggacataca tcggagcgac ttcgatcaga tttccttcta tttcaacagc
     5341 aagagtgctt gagggcattc cctgatactc agcaataccg ccactgaaga ggtagtccac
     5401 cttgctcatg atatttcttg ctctgatttc agaaaagaaa cctttgtctt caagaatttt
     5461 cctgaatgtt tcttcaagcg gagcaagatc agtgttcttt acggtcttga ggagaatgtt
     5521 cccccagccc tcgtaatcaa ggtctgaaaa tacgatcgaa agaagtgatg tcatgaaata
     5581 ttttgcaaaa tcatccatgt tgaagccctt ttcgagtgca tgctgataca taagccagtg
     5641 tgctgatacc gcaggaagtc cgtcgtggtg actttcacct gtgaagcttt catttacgat
     5701 ctcttcgttt tcagcgtgtc taaggcagta atagtcgcct gttttttcat atccgtacct
     5761 tgaaccgtaa gcttcagaaa catattctcc aagacaggga tcacctgaaa tgatgaaaga
     5821 aaaagtgtct gccatacctt cgttgatgcc gagagtttcg ttgctgtaac cgtatttgtc
     5881 aggaaatgcc atatagctca gctttgctgt tccttcgatc gttgcatgac cgaattcatg
     5941 ataaattaca tctccgtcat aggcaaaatc ggcttttgat ccctggccga gaacgatgat
     6001 atcaccttca tatacgaaat tctggaagaa catatcttta aaataagggt cgtgcggact
     6061 gtagaacgca ttatccatcg gagcaagacg ggttgtgctt tccggcatct ggaagttccc
     6121 gacaccgatt ataggattga gctgtcccgc tttgttatgt gttgcaagat gggtgaaatt
     6181 tttgagtccc aggtcgatga gatatttgta tactcttgtc atgtgccagt acacagcgat
     6241 ctcagagtag ttatcttcaa catcaaaact gtacgcaaca cctttgttcc agtcttcgta
     6301 aatgaagttt ccgttgtcga tcttactggc attctgagta ggggtgcaga tcgggtattt
     6361 agaaccgtag taatccatag tatctccaag atcaagacag ttgctcgcaa caacttttct
     6421 tattcctgca tcatcaattt cagcagtgag ctttccttca gcatcatcag ccacccaggg
     6481 gagttcgacc tcgaccgcct ctttgtctct gatagggttt gtgttaaaaa cttttgcagt
     6541 attttcagaa gatttcataa caaaatttcc accgccgaga tatttcccgt catgtgcatc
     6601 gacataatag aaccttccgt ctaggatcga tgacggcttg aaccttattc tgtaagcaag
     6661 acggtatttt ccgaaccgtt caatgatgac aagatttgaa atgaaatcat gatctgcgac
     6721 aatattgccg ttgttctgaa atgaaacttt ttcagccgct tcaacagagg agagatccgg
     6781 cgctgtattt agtgaaaaat cctttaatcc gttgatgaca tttaatactt tccccccctt
     6841 ttcgttgatg acggtgtatc ttccaacgac cggtattccg ttgtagctcc actgtatctt
     6901 gtgaattttc attccagata tttcattcgt tccaaggagc tctcctttga tcgagctgtt
     6961 tttgagcgag atcacactgg gaagttcaag ttttacaaag tttttgagat cggcagatgt
     7021 tctaactgtt ctgttgatgt taaaaaactc agtcttcatc tcttttgcac tgacaaggtt
     7081 tgcaaaaaga ataataattg tcgcggcaag cagtaacttt ttcataattc ctcctgaaaa
     7141 aaacaacatt actgtaatat taactgcaat tgtcatacct catttttttg ttctgttatt
     7201 tcagagaatt agagcgatct cttatctgaa aagtgactgg agtttcctca ctgtatctat
     7261 atgaggcaga tctttataaa ttcctgtaac atatccgttt tccggagtta cgcctcttaa
     7321 aaatatatac cggatgccgc cgaaatcaga ctcatagctg taattttcat cagtttttga
     7381 catatatgtg tcaaaagcgg caagataaag catgtactga agaacatagt tgtgtcgaac
     7441 catctctttt ttaatttcat catcatcata gcacgaatag tcattcccga gcttgttgga
     7501 tttccagtca acaatgtaat atttaccgtt gtgacggaaa acaaggtcga tgaacccgtg
     7561 aatgaaaccg gatattgagc cgccctcaat gtttacagca tcgcctaaaa tatcgctgat
     7621 ctctctgtat gcgaatttct ttgaagggag aaagaactca agctcgctag caatcgaatt
     7681 cttttttaca tttttaagtg aagatccgtc aaaaaccggt gcattaagaa cattttcaac
     7741 acatctctca acccatggaa gcatgtctgc ttctgaagaa tggtatctca tgtagttgct
     7801 gtcaagaatg gaagagatga catcgctgtt gtcaccgctg ctgaaatcga tgttctcaaa
     7861 tatggagtga agaaccgttc ctgccgatgc tcctgccggg aaaagtgaaa tatcgcttct
     7921 caccgcttct tcgctgtggc tgatgacatt ggaaatatgt tcactctgct ttgaagagct
     7981 gatcgcactg aaacttgtaa cagcccactc catgtttatc ccttttttca tttttcttgc
     8041 agggataaga ggggttttgc catactcttt tttcagagct ttcccgtttc ctgaaagatc
     8101 ggtgtcaact tttatggcgc cgccgctttt ttcttcaaga tcattaactt ttttcatgag
     8161 ttcgctgtca gtcatgtttt taaacacatc gtcatcacac acattgaaaa ggatctttga
     8221 aaggggtgcg ctgcctgtag aatttatgtt cccccagtat atgaccgtaa gaaacttcgc
     8281 ccttgtcatc gcgacatata aaagccgcag attttccgac atggtttcga tcctgctcag
     8341 gtctgccgcc gctttatcgt taatgtcaaa tgacagtacc ggcctgtagt tgtcagcttt
     8401 gtcatgatag tggaagtacc acgatttttt cgttccaggt tttttaatga agtaagggat
     8461 gaaaacgatc ttgaaatcaa gccccttgct tttgtgtacg gtcattattg aaacagcgtt
     8521 ctcgtcactt tcaagccgga gttgttcatc ttcgtttttc tcatcaccgg tcattttttc
     8581 cttgacccat gaaagaagcg gttcaaccga tcttcctgcg acggattctc tattgtggag
     8641 aagttcaaaa agatgtctga tgtttgtcag agtcctttcg ccgtatccgg caagaagcga
     8701 atacctgttc tcactgttca tgaattctga aaaagcggtc agaaaccctt tttcctccca
     8761 gttgcggtaa atattggaaa aaagatcaag gtaatgttcg aaaacaggcg gatcatcagc
     8821 aatgtcaatt atctcagacg gtcccatgtt gaagaacatt gtaaggagag cggcttttat
     8881 ccttgacggg atagggtttg ccgcagcttc catgatgatg agaagatcct gcatctcttt
     8941 tgacatgaac accgactggt cggatgtgag aactgcgggg atgccgcttc tttcaaaata
     9001 tgatttgatt tcagttgctt cactgtgctt aatgacaagg atagtgatat ctcccggtct
     9061 gacaggaact ccttcgattg tgtaaccgtt gttttcatca acaagttgaa ggatcttgga
     9121 acatagatca tcaaagacaa tacctttaac gggagcgagt tttatttttt gtttttccgg
     9181 gaattttctg cttcctgtcg tgaatgcaga atatttcgga tcaagtgcgc tcaggtatct
     9241 tatctcaaga ccgtgatgag gaatgtcatt tattttaaga tcgttccggc agtcagcatc
     9301 tttgggttta acgggtttgt atccgatctc gctctcatcg atgatgaaag ggtttttccc
     9361 tgatgagaac agcgcattga cagcttcagt catcatcggg gaagatctga aatttttctc
     9421 catcgaatat tttgtgatgc ttttctgaga tgaacttact ttcagatatg cgaaaacatc
     9481 ggcatttctg aaactgtaaa tagactgctt cggatcaccg atatagaaga cagggttgcc
     9541 ggaagtgccg aaaagttttg tgaaaatact gtattgcacc gggtcggtat cctgaaactc
     9601 atctatcaga acggcgtgat actttttttc cattatcgcg acaagctgtt ttgagaacgc
     9661 atcatttctg ctcaaagccg attcaaggat cgaaagaaga tcgtcaaagc tcatttttcc
     9721 ggttctatcc cgtctgtcga ttatattttt tttagcttca tcaaaaaggc ggtcgacaag
     9781 tatcagtgca tcggtgcaga actctttttc aagggcatga gggtcgcttg tgatctctct
     9841 attcctttca aaactcagct ccgttctttc aacagccggc tttatcagcg gtttttcgca
     9901 aagagtacgg cctttgatca gttttataag ttcatcgaca gagaaccatt tttcttttga
     9961 tgcacggttt atgaactctt catccatata tccgctgtat tttctccaga aatctatcac
    10021 tgcctgctca accggttcag aattctcttt tgaaagttca agattgaatc tcgcacctga
    10081 ttcaaaggcg ttctccctta ctatcctgga acagaatccg tgaatggtga agaccgaact
    10141 tctgtcaaaa tttgtgatgg ctcttctcag ttttttcagt gattcttctc gattgtctga
    10201 atattgtgag gcgatctgtc cgatcagcgt tccgttttga gttttatcgg ttccctcaag
    10261 gatctttttt gccgatctta gaaaatcaag gagacggttt ttaagttcag cggtggcact
    10321 ttttgtgaat gtgacagcaa gaatggattc aaccggagca ccttcaagaa caagccggag
    10381 aaatattgcg gctatcgaat atgtctttcc tgttcccgca cttgcctcga tgaggttcat
    10441 cccctttaac ggaacatctt ttgctgaaaa cccgttcact ttatctcctc gctcagatca
    10501 cttatttttg aaaagatctt tcgtgcagtt ccgatcactt ccgcaatagt ttcatcattg
    10561 ctttcaaaaa aaccggttat gcgggccgcc atatcgaaat agatgtctct ggtcattccg
    10621 ccgaaatcgt catccatttt ttctatcatc atattaaaga tctctctttc acttcttccg
    10681 ggtttattcc cgctgaacag tttttcagct atccacggat tgaacagggg tatttttgac
    10741 ataccgttca gatatatttc aacaaggtcc gaaagctctt cgcagctttt ctgtgacggc
    10801 ttgaaaaaaa ggtcagcatc catgaaagaa aagcaagttt ccttcataac tcctgatgcg
    10861 ttcatggcaa ggtggagtat gagcgctttt actctgtcct tgtctttagg tgtttttgac
    10921 ggtctgaaaa gaacctgtct atccttaaaa acattgtcaa gatcaccgcg aagtgatatc
    10981 tcattctttc cgatgttgaa tttcaggtcc acagaaacag tttcttcagc atccgtccct
    11041 ttcaggtttt ttattttatc aagaaacgca ggcagctctt cgatctggcg gttaagaagc
    11101 actcttcctg tgcttccgtg aggtattaag ccggatcctc tcatttttgt catgaaatct
    11161 tttgcgctaa atcctgcctg attcattttt gcaaattcat ttttcaaacc gtatccctga
    11221 agattatcaa aggtgaaaag ttcaaggtcg tcatcttcct ttctgatata agggaaaacg
    11281 atatccagag tattacggaa aaaatatttc tgaggatttg caaaaaatga gagaagatca
    11341 ttgaccgtta ttttctgcat atttgttttc tgcctgagtt cgggtcttga aacatccaca
    11401 gttccgttaa ccttcccagc cacttgttcc ggtgaaccgg aatggaaatg ttttgcagca
    11461 caaaagtcac gctctgaata ggagaaaaga tttgatcctt ccatgaaata ttttgcgcag
    11521 aagggctgaa gcggatgaag tttttcaatt tgatcgggtt tgacacaata tttttcagtg
    11581 atgtagtttc tcaagctttc aaccactgat gacggaatgt tctttgatga gtcgttcatg
    11641 ctttttgcaa tgtagctgat gacaagcttt tcccttgcac agataagtgc ttcaagaaag
    11701 agatacttgt cattttcctt gacaaaacgg tctgttatcc gcgggtattt gaacatcaga
    11761 tcaaaagcgc tcgacatgtt ttctctgggg aacgattcat cattcattcc tataagagca
    11821 atcactttga aagggatgga acgcatcggc ttaagcgaac agaaagtgac ctgtccgttc
    11881 ataaatcccc gcccgaaacc tgaattggaa aaatagtcat tcaggtgttc tctcaccaca
    11941 ctgagcggaa ctttcccgca aaatcctcct gcagcggtgc ttttgacaag acggttgaat
    12001 gaatcccgca gatatctcaa agtcgcagaa gtttcggcat tatcaataaa gagcagatca
    12061 ataaggcggt tgagtttctc ttcccattga gcaagagttc ttgattcgta aaaatcgttc
    12121 ctgaattcaa atatcaggtc ggtaaaagag atgaactttg caattgttct tgcaattcct
    12181 ccttcgatat catcataagg gagtatgtca ctgaatgttt cagtcccttt ccccggcagg
    12241 gcatatccca gaagcattct gtcaattccg aatttccacg agttctgctc aaattcgtga
    12301 taacctctcg atcttctcca gtttttatct attccccact tgatgccgct ttcggaaacc
    12361 gctttcttta tagattcgag atcttctttt tcaacattga attttctgta gatattgggg
    12421 ttttcaaaaa tagcgatgac ggcagttctt gaaaagttgg tcccgtcaag tgaaagaata
    12481 tttaaaaaag gatcgatcaa tccgctctct tttgttgctg atctatctgc aaccgtgaac
    12541 ggaattaggt ttcctgacga tccgctcccg aaaaccgctt caatgaaatc gctgtattcc
    12601 tcgatttgag gtgccatgac tatcacatca cggggattaa gatcttcatt tgaatcaaaa
    12661 agggacagaa gagtgtcttt aagcacttcc atctctctca tttttcccca gcatccgttt
    12721 atgatgaccg atccgtcaaa atcaagatgg catggtttcg ggttgacgca tgctttaata
    12781 tcgttttgca ggcggtttaa aatggttttg ccttcggtat caggcgcaaa acagtcaatg
    12841 tgctgaccat cggattcaga cagaagattg aaaaagttct ttcctgcaaa tccaagttcc
    12901 gataacagcg ggttatccga ttcataaaaa aatgaatctt tccctgaaaa ttctgtcgtt
    12961 tcaatgttgc cccagaactc gcggctcgga tccatgtgga aaagatggac ttccattatt
    13021 tttgaaaggg atttaaaaag ttcaagctgg aatgcggtca ttgtcgagat tccgaaaagc
    13081 acgacaccct caggcagggg gagtttggaa agatctcttt tttcgatttt atttctgaat
    13141 gcatcaaaaa aagcggctct gtcaagtgag tcgggctttg cagaaattat tgctgaatat
    13201 atctctttct gccagatctc atctttgtct tttttataga cggtttcacc ttttttccac
    13261 tttgcaatta tatcgggtct gaagatctgg tactggtcga acatatctgc aatatgtccc
    13321 gatatctgat atttttttac atcgtctttc cctatatagg tgcggacggg tttcaactct
    13381 tttttgtcga tgaattcagt acttaaaaga tcatagatga accagcggag attcggtgtt
    13441 tcaaaaaacg ggtcagatcc tttttcatca aaaataatgg aggcaatgtg ctgaatgaag
    13501 ttttcaggaa ggatcgtttc aatgtttgca catattcctg atctttctgc gattcttagt
    13561 gaaagccatc ttcccattcc tgaagtctgg actacagcca tatttttttt aagcggatct
    13621 ttataacaaa gcccgtttat gataggaact gctttttcaa caagcatttc gagcatgttg
    13681 ctctgataaa ggaaaaaccc tgaggatgac ggcatgctga attaatttat gatctaatcg
    13741 gtgcagacag ggtagggaag ctgtgagccg gcagcactcg gatcaagcag at
//
LOCUS       DAOOYB010000053        13771 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_4518_length_13771_cov_11.018664, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000053 DAOOYB010000000
VERSION     DAOOYB010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13771
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4518_length_13771_cov_11.018664"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2285)
                     /locus_tag="PL056_04740"
     CDS             complement(<1..2285)
                     /locus_tag="PL056_04740"
                     /inference="COORDINATES: protein motif:HMM:NF012632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46353.1"
                     /translation="MKKFVIIFLMSFLIFSCDKEKTVNDGDETLQDGDVVETQDDDET
                     GIAEPKCADNWEKTDRIDDVSSLFDFQIEKRNDVWDWESNGEYEKLDYSGSINPVYIT
                     DDGTMLLNEFTELEDGYAFMSKLKNDKTVEKFVVKFEKGETISVNGQNSGMHMFSNVI
                     YDGSSNVATAIISMRSKKGLVKPFIVRISSEGDMSYFSWEVTDNSYCGLVDNGEKMIF
                     AICSNWKDGEMGQSIDVYGLSEDSIYKKRIVSGDYAYLSNFFVNGGDLHIDYFALVGE
                     SEETDEMVIIDKDLCSEVKVDTDFFLTDYFRIKKNILTTDFISVSSNFSFLEGVVSES
                     MREGELHYSVGLDFSYFNYYDREIFISLGDRTAPQEDESMFYSFVQTGPVSIVDGKVF
                     KTGSATYDMEGKGRSWVNSEETQQEESGFRAYLEYIDPDTLEVYVRHFILEGMDQHPG
                     QIMVKGDDLFYKPLQAVVKSELIGGTALFKIPKTWLINKDNKAKDDVLKIIEKKLERE
                     NIRKVEQLSTGGRHSCSINEEGRLFCWGDNSDGQMGDAWGEMDGMNEIRNYLPGPVDT
                     SEILMDKQVVDLSAGYLHSCAVNEDGVVFCWGFGRDGQLGNGTYLNVFSPVMADISGE
                     MKEKKIIAVSTGGSFSCGVDESGIVYCWGLNLWGQLGDGTTLQDREDPMPVDMTEALK
                     DRKVVKISSGSGHTCVVADDGGVYCWGLNDRGQLGDGEGGFKGDPTRMADFSRIPVKA
                     ESGELKFSDVSAGYSHTCILS"
     gene            2436..3167
                     /locus_tag="PL056_04745"
     CDS             2436..3167
                     /locus_tag="PL056_04745"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HPM46354.1"
                     /translation="MGFYLKNQYVAIILIVFVTGMSFVSCRKERLNPETYKAREKLVV
                     LGHDFTEEGFMKAIDSKDAGIVKLFMESGMGPDTTVKIKELDIPAIFYALEKGNDLIA
                     QLFIDKGAKLDTSVKGVTVLMKAVEKASVDTLSLILKQSVDVNKPGYQGLTPLMLAIE
                     RNNSGAIWLLLQKGADINLADANGITPLMRALRVGETDIVRELIKKGANVNAVTKNGM
                     KTTKTMNEKNKEALTILLKDAGAQF"
     gene            3185..4333
                     /locus_tag="PL056_04750"
     CDS             3185..4333
                     /locus_tag="PL056_04750"
                     /inference="COORDINATES: protein motif:HMM:NF022646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2891 domain-containing protein"
                     /protein_id="HPM46355.1"
                     /translation="MKYYFPLILIAIFSISLLIITAFFDEDDPEVQENRAFEQIEIRD
                     IPVLTSEMGSKLALLPLSCIKKEYPNKPGHVHESSSSEVAHTLKTPIFSGCFDWHSSV
                     HMHWTLIRLMRLFPDLKQKDQIISLMNEQFTEEKVGTELAFFNSRYNQTFERTYGWAW
                     LLRLQSEFILLNDENGKKWAQTLSPLVKVIRDRSIEYFNKLPKPVRPGTHSNTAFSMD
                     HMLKYAEITNDSEFVESLKKRAVDFFLKDRNCPTDYEPSGIDFVSPCLAQAHLMAQVL
                     EKKAFFNWLNEFLPAPGSDEFSFVMNPPEITDHTDYVIGHLVGLNFQRAWSYIGISNA
                     LDERDYRKELFRSVAAIHLHEGLNGMDKTGYGGEHWLASFATYALTSE"
     gene            complement(4334..4873)
                     /locus_tag="PL056_04755"
     CDS             complement(4334..4873)
                     /locus_tag="PL056_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46356.1"
                     /translation="MKINSAWHITFSGRSLRIDIPGGYVFKRDAIVFTKTAKLGAYIK
                     EDTRIIPEKIAKAMKLRKYIALSEKTVVNAGVFEVRIEDADIYRMRYNISINDSLYFL
                     SFIPDIVKPMSKDSVLLIPADTLYFSIEDLEKIKNFIDLSKAKKIFATGDMSSDLAGM
                     FKPGKITVASEAVQQDIFR"
     gene            complement(4873..6234)
                     /gene="dacB"
                     /locus_tag="PL056_04760"
     CDS             complement(4873..6234)
                     /gene="dacB"
                     /locus_tag="PL056_04760"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HPM46357.1"
                     /translation="MSRFVIPIILLSSLFLSAVELDFLPVNKDDVSILLYDIEKGEEV
                     LDINADKPFYYASNLKLLTSVAALHYLGGGFRFMTLFAFDPAEGTLYIKAAGDPEMVI
                     EKLWVMANDFKRRGIKDIKKVVVDDFIYGPKGFQIAESGDKGDNAYLAFISPIGLNYN
                     AVEIFVKAVEPDKPVEVTLTTPGPHFVINNTAVGTKGGEHGLIVGASAKNGKTEILVK
                     GKLGAGRKKPEIIYKRVGNPLNHFVETLLYLMGEKENIPILREKIPSTVFVREGSINY
                     THKSRPLRDILRDMNLYSSNYLADSIQFFMGAVLKGDSLKGVDILKEYAKVYLDEEID
                     IVNGSGLGNDKNRLSARFYIKLLKKVYGDYYGSIDLFSSLPVMGEDGTLKKASSGSDS
                     TGLIRAKTGSLTGVAALSGLMKAKSGKLYLFAFAVNNFPSKQFKPMWAFRDRIISELW
                     EKY"
     gene            complement(6237..6740)
                     /locus_tag="PL056_04765"
     CDS             complement(6237..6740)
                     /locus_tag="PL056_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46358.1"
                     /translation="MKKNIEDALKTILSNEQVTALKEEIAAGIKKMNSAVESNEKMKA
                     KKDLLVQSLAKVDKLIETSDKSPVLFKAFYSAAKSNIKKFVQPIIEKMIEEEELTGLD
                     DIPDVQEKSDKPDKPETRSAPEKKTEEDPARKNAHKPFKKNKNARHNKPAQAQNKQKN
                     EVKPKGK"
     gene            complement(6749..7342)
                     /locus_tag="PL056_04770"
     CDS             complement(6749..7342)
                     /locus_tag="PL056_04770"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPM46359.1"
                     /translation="MKSSKKNTRKQQILLAASRVFSKKGYHATSISDICAKSGVARGT
                     IYLYFENKRDIFETLIKDFSSAMLENIRMFSLDEPLCDQFDRNIRHFAETIVQNRDLT
                     KIIASEAVGLDSEFDHHLILFYTKLAEYVEGALVMMQKCKEIPDRINARLLAYSIIGT
                     MKEVSYQWSLDCGSILEIDPLIKYLRDFKLKDFIQFK"
     gene            complement(7349..9085)
                     /locus_tag="PL056_04775"
     CDS             complement(7349..9085)
                     /locus_tag="PL056_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46360.1"
                     /translation="MITVDLIGTEMKKNVSEFQALAVTIMNAPASVDADIKAAYEASV
                     QQPTVVLTDTALPKIEEGCPEDAKVLVNLTGEDASTASWAVRIVPSITDSLSDAGSAD
                     EREDLLNIVRQASFDLIQREMMNCSVYLTESAEFNCKIELSVPTQFSKIAYDALLHYF
                     PIDEKSSKVYVKTRKTGMPAIRVICCPDWVNEEWMYWKSRSDEGGDDEPPRIMMIYDI
                     ETNTAFLLGAKSFSEVRKAIKILGWNTAVTTCEALPVNGTAKTVSILKNGKKTETTFL
                     TISNSCGDRAFFGMNVHSADFKTKNEEVLISGDSGMVMLTNIQGRKKSLINFGRNYFG
                     SIDSAVARTSGKPEVISAENVAVAKNDKGDKGVVLNQLFSPEGKVHTLFKQAEKDLNA
                     PEYLVFIVRDELLPPAVMIKDNDLISASWFTYTTECDCCSDMSIVPGGNPNAVWDLSK
                     ELDVFDKALKKTKFKLVMLNSLYFFGQSGEPVEISDEILLSVYTKIAKGEMKWKDWKM
                     MPGYSVPEKNIFKNVKKDFDTVFDPQKITDMDLYTDMMRDSIEMKVDYLRGVCAPAGF
                     IAAFYKILSKLV"
     gene            9299..9832
                     /locus_tag="PL056_04780"
     CDS             9299..9832
                     /locus_tag="PL056_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46361.1"
                     /translation="MRNFLVVLSLLLFSSVLSAQKASFVMKTGKTVSGKITKVEAGAI
                     DSKNLSETSSLNAAQGTNELMIQISDIKEIVFKNHDDVSCFEDGRFVPVRKFCSMKML
                     YHITLKTPSKDKNPIELTDNRVLFFHIEGQKEPVTVFFYKIQVSSEGREDKVDYPDLE
                     REVMSFNKSGVKKITFN"
     gene            9846..10166
                     /gene="trxA"
                     /locus_tag="PL056_04785"
     CDS             9846..10166
                     /gene="trxA"
                     /locus_tag="PL056_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002749437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HPM46362.1"
                     /translation="MLSVNSSNFASEVENFQGLVIIDFWAPWCGPCRMLGPVFEELAS
                     EYTSNEHVKFVKVNTDESPEIARQFMIRGIPTIKFIKNGKEVDQQVGAVPKEVIAGFI
                     SKHL"
     gene            complement(10169..10660)
                     /locus_tag="PL056_04790"
     CDS             complement(10169..10660)
                     /locus_tag="PL056_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HPM46363.1"
                     /translation="MKNCNCGSGKSFAECCEPVLKGKRKPVTAEELMRARYSAFVEKD
                     IDFIMETVAPGSRDSMPRDGVKKWAERNDWKKLEIIKTVKGGKSDDEGYVEFKAHSVV
                     EDVSQVHHENARFIKVKGSWYFEDGEQMLPETVKRESPKTGRNDPCPCGSGKKFKKCC
                     GNS"
     assembly_gap    10775..10874
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10875..11776
                     /locus_tag="PL056_04795"
     CDS             <10875..11776
                     /locus_tag="PL056_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46364.1"
                     /translation="NEGRDFRDYGTIKFDNSELSEAPQLPENLKKEILFLYYYGKNIN
                     YYKILNIPESYNAPQEKIAKRCNCYRQLFAGKNFEGIDLGNYFQKLTKARETIRTASQ
                     IEDPDIRKKYDTFLISISRSRKTDEETKKIKTSSDRASERFGIAMKLLYDKKIQQAYN
                     EILIALHLDPDNKEYLDFKNEISEKLKEEKTNSLFNALEKNDMLLLDESKLENVIDNI
                     LELTENSPVTHLKLAQIALEKEMPEMAIQHAMEAVRKDSDMKNKVAEIVKKAEKQKYD
                     LNTEIEHGPKTYNITRNNIARKN"
     gene            complement(11773..12594)
                     /locus_tag="PL056_04800"
     CDS             complement(11773..12594)
                     /locus_tag="PL056_04800"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPM46365.1"
                     /translation="MDLRKQFQAELFTKVSKGFDQSGPQFFSYFGKVLADIAGINCGF
                     TVLDVGCGRGASLIPAAKLVGNTGKVVGIDIAEGMITELEKDVEKLNLKNIELITGDI
                     EQFEFSDRFDVILCGFSIAAFPDPDRVFNKFRQILKPDGSVAVSNWHKDAHKGWEWMS
                     KAIGDNITSVDTLLKTTGEEYEFDTSETMHIFLKKMGLKNIEVSVIESLFHFRNEEEW
                     WENSLNNGMRGTLDRMDDKSLRKFRKTAFVKLSEMKTEKGIPYPAKVISGVGQNS"
     gene            complement(12629..13765)
                     /locus_tag="PL056_04805"
     CDS             complement(12629..13765)
                     /locus_tag="PL056_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013926403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM46366.1"
                     /translation="MEGAMIPRAYDNLDNYLKKGRVLVIFGPRQVGKTTLLKNFLSKT
                     HLKYRLDNGEDFRIAELFSSRDPDRISEYAKGYELVVIDEAQKIPDARSGLKMLIDNN
                     PDLMVIATGSSSFDLAGTVGEPLTGRKRTLTMFPVSQIELSEMFNRYDLKDMLEEFLI
                     YGSYPEVVEAKSKDEKSRIVTELTHSYLLKDIIAFDRIKNSRVIFDLLRLLAFQTGSE
                     VSLSEIAKNLGIDGKTVGRYIDILEKSFVIFRLTGFSRNLRKEIVKKNKYYFYDTGIR
                     NALIANFNPLDIRNDAGELWENFVLIERFKRNTYKNEILNSYFWRTWNQQEIDLVEEK
                     GGWLSGYEFKYGKKTPKAPDLWTETYSNASFEVINRENYFDFIL"
BASE COUNT         4026 a         3059 c         2401 g         4185 t
ORIGIN      
        1 tcactcagaa tacatgtgtg agaatagccg gctgaaacat cactaaattt caattctcct
       61 gattctgctt ttacaggaat ccggctgaaa tcagccattc ttgttggatc tcctttaaaa
      121 ccgccttccc cgtcaccaag ctgacccctg tcattcaaac cccaacagta aaccccgcca
      181 tcatctgcaa caacacaagt gtgaccggaa ccacttgata tttttacaac ttttctatct
      241 tttaaagctt ccgtcatatc gaccggcatc ggatcttccc gatcctgcaa ggttgttccg
      301 tctccgagtt gaccccataa attcagaccc cagcagtaaa caatgccgct ttcatcaact
      361 ccgcaggaaa aacttcctcc ggtggaaact gcaattattt tcttctcttt catctctcct
      421 gatatgtcag ccatcactgg agaaaaaaca tttaaatatg ttccgttgcc aagctgaccg
      481 tctctgccaa acccccagca gaatacaact ccatcttcat taacagcaca ggaatggagg
      541 tatccggcag aaagatcaac tacttgttta tccatcagga tctcgctcgt atcaacaggt
      601 cccgggagat aattcctgat ttcattcata ccgtccattt ctccccacgc atcacccatc
      661 tgaccgtctg aattatcacc ccagcagaaa agacgaccct cttcattaat tgagcaggaa
      721 tgtctgccac ctgtagacaa ctgttcaact tttctgatat tctccctttc caattttttt
      781 tcaattatct tgagcacatc atctttcgct ttgttatctt tatttatcag ccatgtttta
      841 gggattttaa aaagcgccgt cccgccgatc agttcacttt taacgacagc ttgcaacggc
      901 ttataaaaaa gatcatcccc tttcaccatt atctgaccgg gatgctgatc catcccttca
      961 agaataaaat gtctgacata aacttccaat gtatcaggat cgatatattc aagatatgcc
     1021 ctgaaaccac tttcttcctg ctgcgtctct tcactgttga cccagcttct tccctttcct
     1081 tccatgtcat atgttgcaga accggtctta aaaaccttcc cgtcaactat acttaccgga
     1141 cctgtctgga caaaactgta aaacatgctt tcatcttcct gcggagctgt cctgtcgccc
     1201 aatgaaataa atatctctct gtcgtaatag ttaaaatagg aaaaatcaag accgacactg
     1261 taatgaagtt ctccttcacg catgctttcc gaaacaaccc cttccaagaa actgaaatta
     1321 ctggaaacac taataaaatc agtcgttaat atattttttt tgatcctgaa ataatctgtc
     1381 agaaagaaat ctgtatctac tttgacctca ctgcagagat ctttatcaat tatcaccatt
     1441 tcgtctgttt cttcactctc gcccactaaa gcgaagtaat caatatgaag atctcctccg
     1501 ttgacaaaaa aattgctcaa atatgcataa tcccctgaaa caattctctt tttatatatg
     1561 ctgtcttcag aaagaccata aacatcgata ctttgaccca tctcgccatc cttccagttg
     1621 ctgcagatag caaaaatcat tttttctcca ttatcgacca gaccgcagta tgaattatct
     1681 gtcacctccc atgaaaaata tgacatatca ccttcactgc tgatccttac aataaaaggt
     1741 ttcaccagac ctttctttga cctcatgctg atgatcgctg ttgcaacatt tgaagatccg
     1801 tcataaatta catttgaaaa catatgcatg ccggaattct gaccgttgac gcttatcgtt
     1861 tcaccttttt caaatttcac cacgaatttc tcgacagttt tatcattttt cagctttgac
     1921 atgaatgcgt atccgtcttc aagctctgtg aattcattta acagcatcgt gccgtcatca
     1981 gtgatataaa ccggattgat cgaccctgaa taatcaagtt tctcatactc gccgttactt
     2041 tcccagtccc agacatcatt tcttttttct atctgaaaat caaaaaggga actgacatca
     2101 tctatccggt ctgttttttc ccaattgtct gcacacttcg gttcagcaat tccggtttca
     2161 tcatcatcct gagtttcaac aacatctccg tcctgaagtg tttcatctcc gtcattaaca
     2221 gttttttcct tgtcacagga aaagatcagg aatgacatta aaaaaattat tacgaacttc
     2281 ttcataccgc acctcctgtt gtttttcata tataaacgca agaaataatt tactacatgt
     2341 tataatgaaa gtcaacacct gttccaagaa acatttgaat ataaaaccgt taagtgtata
     2401 atgtttgcgg aactgttttt aagaggggtt ttatcatggg attttatttg aaaaatcaat
     2461 atgttgcaat tatcctgatc gtgttcgtga ctggaatgtc ttttgtttca tgcaggaagg
     2521 aacggcttaa tcccgaaact tacaaggcac gggaaaagct tgtcgtactg gggcacgatt
     2581 tcactgaaga agggttcatg aaagcaatag acagcaaaga tgcaggcatt gtaaaactgt
     2641 ttatggaatc ggggatggga cccgacacga ctgtcaaaat aaaagaactc gacattccgg
     2701 caatttttta tgcccttgaa aaagggaatg acctgatcgc tcaactgttc attgacaaag
     2761 gtgcaaagct cgacacttcg gtgaaaggtg tgacagttct gatgaaagcg gtcgaaaaag
     2821 caagcgttga tactctctcg ctgatactta aacaaagtgt tgatgtaaac aaaccaggat
     2881 atcagggact taccccttta atgcttgcaa ttgaaagaaa taacagcggt gccatctggc
     2941 ttcttcttca gaaaggtgcc gacataaatc tagccgatgc aaacggaata acaccgctga
     3001 tgagagctct ccgtgtcggt gagaccgata tcgtcagaga acttataaaa aaaggggcga
     3061 atgttaatgc tgtcacaaaa aacggcatga aaacgacaaa aaccatgaac gaaaaaaata
     3121 aagaagccct taccatactt ttaaaggacg ccggcgcaca gttctaattt cttcggagaa
     3181 tgcaatgaaa tattatttcc ctctgatttt aatagctatt ttctcaattt cacttctgat
     3241 cattaccgct ttttttgacg aagatgatcc tgaagttcag gaaaacaggg cttttgaaca
     3301 gatagagatc agggatatcc ctgtattaac ttctgaaatg gggtcaaaac ttgcacttct
     3361 tcctctttca tgcataaaaa aggaatatcc caacaagccc ggacatgttc acgaaagttc
     3421 ctcgtctgaa gtggctcata cccttaaaac accgatattc tccggatgtt ttgactggca
     3481 cagctccgtc cacatgcact ggacacttat ccggctgatg cggcttttcc ccgatctgaa
     3541 gcagaaagat cagataattt cattgatgaa tgaacagttc actgaggaaa aggtcggaac
     3601 cgagcttgct tttttcaaca gcagatacaa tcagaccttt gaaaggactt acggctgggc
     3661 atggcttctg cggcttcaga gcgagttcat ccttttaaat gatgaaaacg ggaaaaaatg
     3721 ggcacagaca ctctcccctc ttgtaaaagt tatccgtgac agaagtattg aatattttaa
     3781 caaacttcct aagccggtca gacccggaac tcattcaaac accgcttttt caatggatca
     3841 catgcttaag tatgcggaaa ttacaaatga tagtgaattt gtcgaatcgt taaaaaaacg
     3901 ggctgttgac ttctttttaa aagacaggaa ctgccccact gactatgaac cgagcggaat
     3961 tgattttgtc tctccgtgtc ttgctcaggc acatctgatg gcacaggtcc ttgagaaaaa
     4021 agcttttttt aactggctca atgaatttct tcctgctccc ggaagtgacg aattttcgtt
     4081 tgtcatgaat ccccctgaaa ttacagacca cacggattat gtcatcggac atctcgtcgg
     4141 acttaatttc cagagagcgt ggtcatacat cgggatatca aatgcgcttg atgaaagaga
     4201 ctaccgaaag gaactcttcc gctccgttgc cgcaatacac cttcacgaag gacttaacgg
     4261 aatggataag accggatatg gtggagaaca ctggctggca agctttgcca cctatgccct
     4321 gacatcagaa taattacctg aatatgtcct gctgaacagc ttcgcttgca actgtgatct
     4381 tcccgggctt gaacattccg gcaagatcgc ttgacatatc acctgttgca aagatcttct
     4441 tagcttttga caaatctatg aaattcttga tcttctcaag atcttcgatg ctgaaataga
     4501 gtgtgtcggc cgggatcaga agaaccgaat cttttgacat cggctttacg atatcgggga
     4561 tgaacgaaag aaaatagagt gaatcattta ttgaaatgtt atacctcatg cggtaaatgt
     4621 cggcatcttc gatcctgact tcaaaaacac ctgcgttcac aaccgttttt tcagaaagag
     4681 cgatatattt tctaagcttc atcgccttag caattttttc agggattatt ctggtatcct
     4741 ctttaatgta agcaccgagt tttgcagttt ttgtaaacac aatggcatcc cttttaaaaa
     4801 catatccgcc cggaatgtct atcctcagcg atcttcccga aaaagttatg tgccatgctg
     4861 aattgatctt catcaatatt tttcccaaag ctctgagatt atcctgtcgc ggaaagccca
     4921 catcggctta aactgttttg aaggaaaatt gtttactgca aatgcaaaaa gatagagttt
     4981 tccgcttttt gccttcatga gacctgaaag ggcggcaact cccgtaagag agccggtctt
     5041 tgccctgatc aacccggtac tgtcacttcc tgaagaggcc tttttcagtg tcccgtcctc
     5101 acccatgact ggaagtgatg aaaaaaggtc tatactcccg tagtaatcac cgtaaacctt
     5161 tttcagaagc ttgatgtaaa atcttgcact cagccggttc ttgtcgttgc caagcccgct
     5221 gccgttgaca atatcaattt cctcatcaag atacactttt gcatattctt taaggatatc
     5281 aacgcctttc aatgaatcac cttttaaaac ggcccccatg aaaaactgaa tgctgtcggc
     5341 cagataattt gacgaataca ggttcatatc cctaagaata tcgcgcagag gtctgctctt
     5401 atgtgtgtag tttatcgatc cttccctgac aaaaacagtt gacgggattt tttctcttaa
     5461 aatcggaatg ttttcttttt cacccatcaa ataaagcagt gtttcaacaa agtggttcag
     5521 tggatttccg acccgtttat aaattatttc gggttttttc cgtcctgcac ccagtttccc
     5581 tttcacaagt atttcggttt tcccgttctt tgcacttgcg ccgactatca gaccgtgttc
     5641 accccccttt gttcctacag cagtgttatt gataacaaaa tgaggtcccg gagttgtcag
     5701 cgttacttca acaggtttgt ccggctcaac tgctttcacg aatatttcca cagcattata
     5761 gttaagaccg attggtgaaa tgaaggcaag atatgcatta tctcctttat ctccggattc
     5821 agcgatctga aaacctttcg gtccgtaaat gaaatcatca actacaactt ttttaatatc
     5881 ctttatcccc cttcttttga aatcatttgc cataacccag agtttttcaa taaccatttc
     5941 aggatcgccg gccgctttga tgtaaagtgt tccttccgca ggatcaaacg caaaaagtgt
     6001 catgaacctg aacccgccac ccagatagtg gagtgcggca accgaagtaa gaagctttag
     6061 atttgaagca tagtaaaaag gtttatcagc gttaatatcc agaacttcct cacctttttc
     6121 gatgtcataa agaagaatcg aaacatcatc tttattaacc ggcagaaaat ccagctctac
     6181 agcagataag aatagagaac ttaataatat tattggaatt acaaaacggg acatagttat
     6241 tttcctttag gttttacttc gttcttctgt ttattctgag cttgtgccgg cttattgtga
     6301 cgagcatttt tgttcttttt aaacggctta tgagcatttt tccgggcagg atcttcttca
     6361 gttttcttct caggagcaga tcttgtttcc ggcttatccg gcttatcaga tttttcctga
     6421 acatccggaa tatcatcaag tcccgttaat tcctcttctt ctatcatttt ttcgattatc
     6481 ggctgaacaa acttcttaat attggacttt gccgcagaat aaaaagcttt aaaaaggacc
     6541 ggcgacttat cagatgtttc tataagttta tcaacttttg caagtgattg aacaagaagg
     6601 tcttttttag ctttcatttt ctcgttgctt tccacagcgg aattcatctt ttttattccc
     6661 gcagcgatct cctctttcaa agccgttacc tgttcattcg aaaggatcgt cttaagtgca
     6721 tcttcaatat tctttttcat tttcacacct atttgaattg aataaaatct ttcagcttga
     6781 aatctctcaa gtatttgata agaggatcta tttcaagaat actgccacaa tccagagacc
     6841 actgatacga aacctctttc attgttccta ttatcgaata tgcaagcagc cgtgcgttta
     6901 tcctgtccgg tatttctttg catttctgca tcataacaag agccccttcc acatattcgg
     6961 caagttttgt gtagaagagt atcagatgat gatcaaattc gctgtcaaga ccgaccgctt
     7021 cgctcgcaat gatcttggtg aggtcccggt tttgaactat cgtctcggca aagtgcctga
     7081 tatttctgtc aaactggtca cagagaggtt catctaaact gaacattctg atgttttcaa
     7141 gcattgctga agaaaagtcc tttatcagcg tttcaaaaat atctctttta ttctcgaaat
     7201 agagatagat ggttcctctc gcaacacccg attttgcaca aatatccgaa atacttgtag
     7261 catgataacc cttttttgaa aaaaccctgc tcgcagcaag aagtatctgc tgtttccgtg
     7321 tgtttttctt ggagcttttc atattaaact acacaagttt ggacaatatt ttataaaatg
     7381 cggcaatgaa acctgcggga gcgcaaactc ctctcagata gtcaactttc atttcaattg
     7441 aatctctcat catatctgtg tagagatcca tgtcggttat tttctgcgga tcaaaaactg
     7501 tgtcaaaatc ctttttcaca ttcttaaaga tgttcttttc aggaactgaa tagccgggca
     7561 tcattttcca gtctttccat ttcatttcgc cctttgcaat tttagtgtac acagaaagaa
     7621 gtatttcatc acttatttca accggttcgc ctgactgacc gaagaaataa agactgttga
     7681 gcataacaag tttgaacttg gttttcttga gagctttgtc aaaaacatca agctcttttg
     7741 aaaggtccca cactgcattg ggattaccgc cgggaacaat gctcatatcg ctgcagcagt
     7801 cacactcggt agtataagtg aaccatgaag cggatataag atcattatcc tttatcatga
     7861 cagccggggg aaggagttca tctctgacaa taaaaacaag atattccggt gcgttaagat
     7921 ccttttctgc ctgtttaaaa agagtgtgca ccttcccttc gggagaaaac aactgattta
     7981 aaacaactcc cttgtcacct ttgtcgtttt ttgcaacagc aacattttca gccgatataa
     8041 cttcgggttt acctgaagtt cttgcaacag cactatctat tgaaccgaaa taatttcttc
     8101 cgaaatttat cagagatttc tttcttccct gaatatttgt aagcattacc atgccggaat
     8161 caccgcttat aagaacctct tcatttttag ttttaaaatc tgcgctgtgg acattcatgc
     8221 cgaaaaacgc cctgtcaccg caactatttg aaattgtgag gaaagttgtt tccgtcttct
     8281 tgccattctt caatattgaa actgtttttg ccgtaccgtt gacaggaagg gcctcacatg
     8341 ttgtaacagc ggtattccat ccaagtatct tgatggcttt ccttacttct gagaaacttt
     8401 ttgcaccgag aaggaaagcg gtatttgttt caatgtcgta tatcatcatt attcttggcg
     8461 gctcgtcatc tccaccctcg tcagatcttg atttccagta catccactct tcatttaccc
     8521 agtcaggaca gcaaattact cttattgccg gcatacctgt cttgcgggtt tttacataaa
     8581 cttttgaact tttttcatct atcgggaaat aatgaaggag cgcgtcataa gcgatcttgg
     8641 agaactgtgt cgggacagaa agctctattt tgcagttaaa ttcagcactt tcggtaagat
     8701 acactgaaca gttcatcatt tctctctgaa taagatcaaa agacgcctgc cttacgatat
     8761 ttaaaagatc ttctctttca tcggcactgc cggcatcact taaagaatca gttattgaag
     8821 gaactatcct aaccgcccag ctggctgtgg aagcatcctc gcctgtgaga tttacaagaa
     8881 cttttgcatc ttccggacac ccttcctcga tttttggcag agctgtatcg gtaagtacaa
     8941 ctgtgggctg ctgaacagaa gcttcgtacg ccgcctttat atcagcatcg actgaagcag
     9001 gagcattcat aattgtaacg gctaatgcct ggaactccga aacattcttt ttcatttcag
     9061 ttccgatcaa atcaactgta atcatctggt tactccatga tattattggt aaaaacatta
     9121 aaaagccgtc aaattataca aaaaatataa ataatatcaa ataaatgttg ctgactagtt
     9181 tcagtttttt attcatactg tatatcatct tgttaatttt ttgcattcac tcttgccatt
     9241 tttgatattg atactataat gtacgctggt ttgttgaaac aattttccgg aggttaatat
     9301 gagaaatttt ctggtagttt tgtcattatt actgttttca tccgttcttt ctgcccagaa
     9361 agcgtcattt gtcatgaaaa ccggcaaaac cgtcagcggc aaaataacaa aggtggaagc
     9421 gggagcgatc gacagcaaga atctctcaga aacctcgtct ctcaatgctg cgcagggaac
     9481 aaacgaactg atgatccaga tcagtgatat caaggagata gtgtttaaaa accatgatga
     9541 tgtgtcttgt tttgaagacg gcagattcgt tcctgtaaga aagttctgct cgatgaaaat
     9601 gctctatcat ataactctta aaaccccgtc aaaagataaa aatccgatcg aactgacaga
     9661 taaccgtgtt ctttttttcc atattgaggg acagaaggag ccggtaacag tctttttcta
     9721 taaaattcag gtcagcagtg aaggtcgtga agataaagtt gactatcccg atcttgaaag
     9781 agaggtcatg agttttaaca agagtggcgt aaagaaaata acttttaatt aacaggagaa
     9841 ttatcatgtt atcagtaaat tcatctaatt ttgcttctga agttgaaaat ttccaaggac
     9901 ttgtaattat tgacttctgg gctccctggt gtggaccatg cagaatgctc gggccggtat
     9961 ttgaagaact tgcttcagaa tacacatcta acgaacatgt taaatttgtt aaagttaaca
    10021 ctgacgaaag ccctgagatc gcaagacaat tcatgatcag gggaatcccg acaataaaat
    10081 tcattaaaaa cggaaaagag gttgatcagc aggttggtgc cgttccgaaa gaagttattg
    10141 ccggatttat atcaaaacat ctttagtatc aactgtttcc gcaacatttc ttgaattttt
    10201 tgccgcttcc gcaagggcat ggatcgtttc ttcccgtttt cggggactct ctcttaactg
    10261 tttcaggaag catctgctca ccgtcttcaa aataccatga gcccttaact tttatgaatc
    10321 ttgcattttc atgatgcacc tgtgacacat cttcaacgac cgaatgggct ttgaactcga
    10381 cataaccttc atcgtcactt ttgccccctt taacggtctt gattatttcg agctttttcc
    10441 agtcgttcct ttcagcccac ttcttcacac cgtccctggg catgctgtcc cttgagccag
    10501 gcgcaaccgt ttccataata aaatcaatgt ctttttcaac aaatgcagaa tatctcgccc
    10561 tcatgagctc ttccgctgtc accggcttcc tctttccctt caataccggc tcacaacact
    10621 ctgcaaaact ctttccgctg ccgcaattac aatttttcat gacctactcc cgtttaacaa
    10681 agaaaatatc ggacagatta tattctgact cgtttcataa tcaaaaaaaa gatttttgaa
    10741 ttaaaaagta aatgttatgt gtaaaatttg acaannnnnn nnnnnnnnnn nnnnnnnnnn
    10801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10861 nnnnnnnnnn nnnnttaatg aaggaagaga ttttcgtgac tacggaacaa ttaaatttga
    10921 taattctgaa ctttcagaag cacctcaact gcccgaaaat cttaaaaagg agatcctgtt
    10981 tctctattat tacggaaaga acatcaatta ttacaagata cttaacatac ctgaatcata
    11041 caatgctcct caggaaaaga tagccaaacg atgcaactgc tacagacagc tttttgcagg
    11101 taagaatttt gaaggaatcg atcttggaaa ctattttcag aaacttacaa aagcaagaga
    11161 gaccattcga acggcttccc agatagaaga ccccgatata agaaaaaaat atgacacttt
    11221 tctgatttct atatcccgct caagaaaaac tgatgaagaa acaaagaaga taaaaacgag
    11281 ctcagacaga gcaagtgaaa gatttggaat cgcaatgaaa cttctttatg ataaaaagat
    11341 ccagcaggca tacaacgaga tccttatcgc tctccacctt gatcctgaca acaaagaata
    11401 tctcgacttt aagaacgaaa tttccgaaaa gctcaaagaa gaaaaaacaa actctctttt
    11461 caatgctctt gaaaagaacg acatgctcct gctcgatgag tcaaagctgg aaaatgtcat
    11521 cgacaacatt cttgaactga cagaaaattc tcccgtgaca catctgaaac tggcacagat
    11581 cgctcttgaa aaagaaatgc ctgagatggc tatacaacac gcaatggaag ctgtaagaaa
    11641 agactctgac atgaaaaaca aagtagctga aattgtaaaa aaagctgaaa aacagaaata
    11701 tgatttgaat acagaaatcg agcacggacc taaaacttat aatattacca gaaacaacat
    11761 cgcaagaaaa aattagctgt tttgcccaac ccctgatatc actttcgcag gatatggaat
    11821 tcctttttca gttttcattt cagatagttt tacgaaagca gtttttctga attttctcaa
    11881 tgacttatcg tccattctat caagagtgcc cctcattccg ttattaagtg aattctccca
    11941 ccactcctct tcatttctaa aatgaaataa cgattcaatc actgaaacct caatgttttt
    12001 cagtcccatt ttttttaaga atatgtgcat agtttcactg gtgtcaaatt catactcttc
    12061 gccagttgtt tttaagagcg tatcaactga agttatgtta tctccaatgg cttttgacat
    12121 ccattcccac cctttatggg catctttatg ccaatttgaa acagcaactg atccatccgg
    12181 cttaagaatt tgtctgaatt tattaaaaac cctgtccgga tcagggaatg cggcaattga
    12241 aaaaccgcag agaataacat caaatctgtc gctgaattca aactgttcaa tgtcaccggt
    12301 aatcagttca atgtttttga gattcagttt ttcaacatct ttttcaagct cagttatcat
    12361 tccctccgca atgtcaattc cgacaacttt cccagtgttt ccaacaagtt ttgcggcagg
    12421 gatgagagat gcaccgcgac cgcaaccgac atcaagaacc gtaaacccgc aattaatacc
    12481 ggcaatatca gcgagaactt ttccaaaata agaaaaaaac tgcggacctg actgatcaaa
    12541 accctttgaa acttttgtaa acaactcagc ttgaaactgc tttcttaaat ccattgtcct
    12601 ttcccttccg gtcctgaggc aaaacagatc acaaaataaa atcaaagtaa ttttctctgt
    12661 ttatcacttc aaatgatgcg ttactgtaag tttcagtcca gagatccgga gctttcggag
    12721 ttttctttcc atatttgaac tcatatccgg atagccaccc ccctttttct tcaacaagat
    12781 caatttcctg ctgattccat gttctccaga aatagctgtt aagtatttca tttttatatg
    12841 tatttctttt aaatctttca attaatacaa aattttccca aagttctccc gcatcgtttc
    12901 ttatatcaag tggattgaaa ttggctatca gagcatttct tattccagtg tcataaaaat
    12961 aatacttgtt ctttttgacg atctctttcc tgagatttct gctgaaaccg gtaaggcgga
    13021 atatgacaaa cgatttttcg agaatatcaa tatatcgtcc aactgttttt ccatcaattc
    13081 ctaagttttt tgcaatttca gaaagtgaaa cttcgctccc tgtctgaaag gcgagaagcc
    13141 ggagaagatc aaagatcaca cggctgttct ttattcggtc aaaagctatg atgtctttaa
    13201 gaagatatga atgtgtaagc tctgtaacta ttcgtgactt ctcatctttt gactttgctt
    13261 caacaacttc agggtatgat ccgtaaataa gaaattcttc aagcatatct ttcaggtcat
    13321 atctgttgaa catttctgaa agctcaatct gagagacagg aaacatggtc aaagtccttt
    13381 ttcttccagt cagaggttct ccgacagttc ctgcaagatc aaaagaggaa gaaccagttg
    13441 caataaccat caaatcagga ttgttgtcta tgagcatttt aagtcctgaa cgggcatcgg
    13501 ggattttctg agcttcgtct atcacaacaa gttcatatcc tttagcatat tcagaaattc
    13561 tgtcgggatc tcttgatgaa aaaagctctg cgatccggaa atcctcacca ttatctaaac
    13621 ggtatttaag atgtgttttt gataaaaagt ttttcaaaag agttgtcttt ccaacctgcc
    13681 ttggaccaaa aataaccaga acccttcctt ttttcaaata attatcaaga ttgtcataag
    13741 ctctcggaat catggctccc tccataaaaa a
//
LOCUS       DAOOYB010000054        13695 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_4548_length_13695_cov_12.117889, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000054 DAOOYB010000000
VERSION     DAOOYB010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13695
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4548_length_13695_cov_12.117889"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..905)
                     /locus_tag="PL056_04810"
     CDS             complement(<1..905)
                     /locus_tag="PL056_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46367.1"
                     /translation="MTLKKVLVLLFAALFILPLSAADDFDDEFGGFDDFEDEGKKKDV
                     DDEDYDAEENAEKKKERDQEASDAAMKDLLGDEEEIIEKETEKEDRKESKKSSKSDEP
                     KKEKKKAEPKEKGLKPVILIKGGFTLLGQYKTAPKTTLGSMFGGVDEGLLGAEFIGDH
                     VIAKGTMNIRTDNPLIDKGNNPLAKKNLHTIQNGAENGLYELYGGLKFFDIFVKAGKM
                     LPEYGLVDTYQTIGMGFTTPFLTRSLIAVEGFIPETDAGIALGYKGTFADAHTILVGL
                     MLGTGSIASDYWNSDKTMGLYGRVG"
     gene            1103..1771
                     /locus_tag="PL056_04815"
     CDS             1103..1771
                     /locus_tag="PL056_04815"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPM46368.1"
                     /translation="MKRIFKMFAVFSLVFSVIAGCAGAGSIEKKPDPVEVTHKVILET
                     GEGRIVLALFGNGMPQTVKNFVDYVNSGFYDGLIFHRVVKNFVIQGGGFSAEMVQKET
                     KPPVKFEMPPFEEVVSESGKKSKKLLITHEKYALAMARTRDPHSATSQFYITLAPTKQ
                     LDPNPEYSEPNGYAVFGKVVEGFEIIDRIGSVEVGMQKGHANVPVEPVKIIKMSLMAQ
                     GEKK"
     gene            1768..2145
                     /gene="queD"
                     /locus_tag="PL056_04820"
     CDS             1768..2145
                     /gene="queD"
                     /locus_tag="PL056_04820"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HPM46369.1"
                     /translation="MKFEVYREIHFSSAHRLRNYCGKCENIHGHNWRVRLCVTRKELD
                     KTGFVMDFKDIDRILKKITDFLDHKDLNALEQFHEINPTAENIALFIFNEAEKEVAAV
                     DREASVSKVYVWESERSCACVGE"
     gene            2147..2590
                     /locus_tag="PL056_04825"
     CDS             2147..2590
                     /locus_tag="PL056_04825"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPM46370.1"
                     /translation="MNFRKNVAAVIVDDKGLILVGKRTDVADAWQLPQGGVDKDENEQ
                     YAVLREVFEETGIQPHFLNVVKKTSVIRYLFPPEIRKGMKFDGQEQIYFLIKIVGPGW
                     SLERSDEFSDFEWLDQNEVVKRAVEFKKNSYIEAFRQLFGVKDGI"
     gene            2580..5192
                     /locus_tag="PL056_04830"
     CDS             2580..5192
                     /locus_tag="PL056_04830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HPM46371.1"
                     /translation="MASEKKKKKSIIRKIAELKKTIMMLILISALSGFLISVAVYYKY
                     KAEIPSINTVSDYKPDAGTKVYSYDGELIAEFYHENRRIIVPYDKIPEKLRLAFVAGE
                     DSSFYEHSGIDLFGMMRAGFKYLVTGVKQGGSTITQQLAKSFVGTERTWGRKIKDIIL
                     AIELEKHLSKEEILYLYLNEIYLGSGSFGVEAASRKYFSKHVWELDLHEIAVLAGLPK
                     FPSLANPIQNPKTALRRRNYVLKRMFEEKYITEAEYEKAKNEPVVTDPQKELFLDKSP
                     YFSERVRRYLLEKYGFDKLYKEGMSVQTTVDMRATKLAHEAVFMGLRELSKRQGYARS
                     TENSYQKSKKFKGEVTNPLYKINLKKDLEKYLLRHEEHFGEVTESNISSGIYYQGVVI
                     EIKPKEAVIQVGKVKRPVYLEDLMWARPWNPTGYPPVMREMSDVLSPGDVVMVRATDK
                     EGTRDVFDHRNYSKKLKKAFFFVLEQIPASQAAIIVKDPYSGYVKALMGGYDFEISEF
                     DRTTQACRQPGSAIKPVFYSLALEKQGGRPPLLTPASMILDAPVTVVDTNFKPSNFEN
                     TFKGEITVWEALIHSMNTPAIRVLQKTGLADALKWAQNLGIKSQIRPEFGSMLGASCL
                     TIDELTDAYAHFPNMGKSPYTTYIRKVYDKYGKVLEDNTVFYDPYISAPEKIARLVYF
                     AKREENQVVTPQTAYIVNKVLEDVVNRGTAGSARGLNNYKCNKKPNPAEVTESSEQVE
                     ESSYCSRHVAGKTGTTNDYFDAWFIGYSPNLVAGVWVGNDQHGKPIGRVETGGKAALP
                     VWMDFMGRYLANSEPTDYKIPYGIVSRAIDTATGLVTESGGVRMYFHSGSEPQGSVDE
                     KHILDPTKGPEGIF"
     gene            5189..6793
                     /gene="murJ"
                     /locus_tag="PL056_04835"
     CDS             5189..6793
                     /gene="murJ"
                     /locus_tag="PL056_04835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HPM46372.1"
                     /translation="MSTRSILKSSYVLVGFTLISRFLGLARESVKAAMLGTTALSDAF
                     SVAFLIPNLLRRLFAEGTISAAFIPTFKGYLSRNDKKETADFLSAVFTFGITVFAVTV
                     FIGIGLSGALVKIFGPAFSQDVYSEAVFLTQIMWPYLAFISVAALLQGVLNSLGIFGP
                     SGFTPILFNLSFITAAFILSPFTANPARAIAIGVTAGGIIQMLFQIPFMMKTGMRFSF
                     TNLKKAFFHPGVRKVMRLISPTLLGMGAYQINIIATTHIANRAGEGIVSSLQFSNRLE
                     ELILGVFIVSLSTVILPELSTDAKKGDWENFNKNLVFGIKVTAMISIPATVFTLLMRE
                     EIVSLLFKFGRFDDESVRLTSYALMFHISGLFFIAVTRILFPAFFAQEDTVSPTVAGI
                     VSVVVNIVAAYLLVGSMKGGGIAFASTISAFVSTVILVFMLRKGGKINMNAVLSSFSY
                     AFRFLLISVCCGVPLYFTKDFVYGVFSKIPAMSAFGEKIIREFVPFSIITAIFGIFLV
                     AVLIIIKDENAVYIFDKVARRLKWKK"
     gene            6781..8148
                     /gene="lpdA"
                     /locus_tag="PL056_04840"
     CDS             6781..8148
                     /gene="lpdA"
                     /locus_tag="PL056_04840"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HPM46373.1"
                     /translation="MEKVKLLIIGAGPGGYVAAIRAAQLGLEPVVIEKADLGGTCLNW
                     GCIPTKALIAAAETFENINHSDAFGITATATFDWTKVVAHAKNAVTKNRAGVQYLFKK
                     NKVRLVTATAKFISPKTVKAGETEFEAENIIIATGSKVRDVAGFPVDGKNIISSDHAL
                     FQEKLPKSIAIIGGGVIGVEMAYIYHSFGVEVTIIEAMNSIIPFEDADISKELTKEFK
                     KKKMKIHTGVFVEKVEKVNDLMVITLNNGTTVEAAQVLSSVGRSPNLDGLDIEKGGVV
                     LNEKGFIKTDELFKTNVEGVFAIGDCIPTPMLAHTAEHEGIVAVEQIAGMKVHKIDYL
                     LNPGCIYCLPSIGSIGYRESTAIEKGIKIKTGKFPFSANGKAVASNHTTGFVKVIIEE
                     ESHKIIGAHIIGYGATDLISEFILAMKLGATAEDIIEAIHPHPTLCEASLEAVLNSLG
                     RTIHI"
     gene            8159..9802
                     /locus_tag="PL056_04845"
     CDS             8159..9802
                     /locus_tag="PL056_04845"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HPM46374.1"
                     /translation="MYQLLMLPAGLAFFLFGMTQLSSMLSGFTGKSFADKLDKWTRND
                     FTKIFTGFTITTLFQSSGATTAILVSMTSVGLVTVTSSVSYLIGANIGSITTAWIVAV
                     KVTSFGVFMFTSGFLLKFVLKNEKLKKISIFVAGLGLIFFGLQMMMDSVSFLKENQKA
                     LSFFSGFDASSSFISMLILVIAGVVFTAVIHSSGATAAMIIGLVSTGAVSLHSGAAVV
                     LGSTLGTTATAYLASLGTSAEGKRTAFLQIFINVIELLAGLVFFYPMVELVLTISKTY
                     SFDAGFMIALYMTFLKIILAVIVFPLRKVFAVTAEKFIRKKFRLITESLNISPVTADD
                     TPEKIKEKFEPVIELCVKYITDMLSYSYIGIRKPALRSIYKKVIHYEEIIDEAQKDIV
                     EIISKSRNSHTDILWLFLKMADELESMGDHAKSIAKYGVRLDEIRHKLNDEQKKILLE
                     CYLMVFRQFHEVCVKKNFDCALVAKGEEIERHLRREKRKIYSLLCTESDHNYEKRLIL
                     VDILSEYSKFNHSVKRILQVNIDVSEGKGIYLWESRCKN"
     gene            9803..10528
                     /locus_tag="PL056_04850"
     CDS             9803..10528
                     /locus_tag="PL056_04850"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HPM46375.1"
                     /translation="MTFTERTDLVFGADGIEKLKNSTVAVYGLGGVGASAAVSLVRAG
                     VGTIAVTDFDKVNISNLNRLVFGFESTLNLDKTEVFVKTAKDINPDIKIIISKDFVHG
                     ADAAQKVHSADFHIDAIDSLNPKVNLIIALLENNAAFISSMGTGGKFRPELLQISDIW
                     KTDVCPLSKFVRKRLRNRGIRKGFPVVFSKELSTEPVDLEDDHNEEMPGRKRLTQGST
                     PFVPQAAGLILASYAVRKLLGKG"
     gene            10615..11643
                     /locus_tag="PL056_04855"
     CDS             10615..11643
                     /locus_tag="PL056_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HPM46376.1"
                     /translation="MRKDKKKESNQSQIILYTTPDGDIKIDTILQDETIWLNQSAISE
                     LFDVKIPAVSKHLKNIFESGELDEKVVVSILENTTKHGAIEGKTQKKEVKYYNLDVVI
                     AVGYRVNSKRATQFRIWAASILKEYILKGFVMDDDRLKNLGGGGYWKELLQRIRDIRA
                     SEKVFYRQVLDIYATSIDYDPKAEVSIEFFKKVQNKIHYAVHGQTAAEVIFNRADSEK
                     EFMGLMSFPGSRPYLKDVVVAKNYLNEKELRSLGQIVSGYLDFAERQAEREQAMTMKD
                     WAEHLDKILTMSGEKLLLGAGSVSHETATEKAISEYKKYQQKTLSEAERNYLESLKTL
                     ESKSKNQK"
     gene            complement(11881..12849)
                     /locus_tag="PL056_04860"
     CDS             complement(11881..12849)
                     /locus_tag="PL056_04860"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HPM46377.1"
                     /translation="MSNLSRITFIDERIRSRGFVTRKEVAEEFEVHIDTVKRDIEYMR
                     LFLNAPLEYSNKFHGYTYSTPFETVFSAAEDAALYYIFVHRITDGFRLGGVPYVPVIS
                     KDILTKIKSYVPSEYENILDSVVYDSSDQDIMDIRDFRNILRSIKSSLQLEIGYCNAK
                     GEISSRVVEPMLLTNYLGKWYIIGYCHKRKELAVFLISRITNCRVTENMFENAVTKKE
                     LKNYTDGSFGMFKSSKTALATIRISEPSMHYIRNQKWHKDQIVIEGSSKGINYIEITL
                     PVGERHNEIIARTMAYAPDSEIVAPKELRDKWLDTIKKMWNKYGTL"
     gene            complement(12885..13100)
                     /locus_tag="PL056_04865"
     CDS             complement(12885..13100)
                     /locus_tag="PL056_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46378.1"
                     /translation="MLNLFKRIFGFDADEVHLFGASFKERIEEGLEIIAEGIEDISKA
                     ASSFGEEDSETVDSLEDVTEEVEIIEP"
     gene            complement(13093..13665)
                     /locus_tag="PL056_04870"
     CDS             complement(13093..13665)
                     /locus_tag="PL056_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46379.1"
                     /translation="MKKNLPVPQELRARLKMMSGYHEAKNALSQSRFPVVKKSYVIKS
                     DGDEIDEMTDRVNGILEGEITKLRDHALGVVERFVNSLEVAMCDFLKEHGVEPVDLVL
                     GNDPFWRLKELPMVITTYGFEERLIFKGLGDLLLTRGKIMGLDEYPGFAVSALDNVFE
                     AGVSSVFKKMGIIIKDEKKTELLTAEGSDA"
BASE COUNT         4087 a         2660 c         3026 g         3922 t
ORIGIN      
        1 tatccgacac gaccgtaaag acccattgtc ttatcactgt tccagtaatc ggaagcgata
       61 gaaccggttc caagcataag gccgacaagt attgtgtggg cgtcagcgaa tgtccctttg
      121 taaccaagtg caatacctgc atctgtctca ggaataaaac cttcaacggc aatgagactt
      181 cttgtgagga aaggagttgt gaatcccatt ccgatcgtct gataagtgtc aacaagaccg
      241 tattcaggaa gcatttttcc tgcttttaca aatatgtcga agaacttaag tccgccgtaa
      301 agttcataaa gaccgttctc agcaccgttc tggatggtgt gaaggttctt ttttgcaagg
      361 gggttgttcc ctttgtcgat aagaggattg tcggttctga tgttcattgt tccttttgcg
      421 atgacatgat caccgatgaa ctctgcacct aaaagacctt catcaacacc gccgaacatt
      481 gaaccgagag ttgttttcgg agcggtttta tactgaccga gaagtgtgaa tccgcctttg
      541 ataagaataa ccggcttcag ccccttttct ttgggttcag cttttttctt ttcctttttc
      601 ggttcatcag attttgatga tttttttgat tctttcctat cttccttttc agtttctttt
      661 tcgatgatct cttcttcatc gccaagaaga tctttcattg cggcatcgct tgcttcctga
      721 tctctttctt tctttttttc tgcattttct tcagcatcat agtcttcatc atcgacatct
      781 ttcttttttc cttcgtcttc gaaatcatcg aatccgccga attcatcatc aaaatcatct
      841 gcggctgata atggaaggat aaaaagagct gcaaacaaca gtaccaatac tttcttaaga
      901 gtcattaatt cctccgtcat agctaaaaca aataaatctc tatttttaat aacagtcaaa
      961 aaatctttag acaccttatt ggaacatctt ggaaaattca agttttttta acattaaact
     1021 gagtttatac ttattttctt tgaaagaaat agagttttgt gatattttgc cggtgttgct
     1081 gtttgttgca ggaggaatca aaatgaaacg gatttttaaa atgtttgcgg ttttttcttt
     1141 agttttttca gttatagcag gatgcgccgg agcgggaagc attgaaaaaa aacctgatcc
     1201 ggtggaagtt acacacaaag ttattcttga aacgggtgaa ggcaggatcg ttcttgcact
     1261 tttcggcaac ggaatgcctc aaactgttaa gaattttgtt gattatgtga acagcggttt
     1321 ttacgacggg ttgatcttcc accgcgtggt gaagaatttt gtaattcagg gtggcggttt
     1381 ttcagccgag atggtgcaga aagagacaaa gccacctgtg aagtttgaga tgcccccttt
     1441 tgaagaggtt gtttcggaat cggggaaaaa atcaaaaaaa cttctgatca ctcacgaaaa
     1501 atatgctctt gcgatggccc gaacaagaga tcctcacagt gcaacaagcc agttctatat
     1561 aactcttgct ccaacaaagc agcttgaccc taatcctgaa tacagtgaac ccaacggata
     1621 tgctgttttt ggaaaagttg tcgagggttt tgaaattatt gacaggatcg ggtctgtgga
     1681 agttggcatg cagaaaggtc atgcaaatgt tcctgttgag ccggtgaaaa taataaaaat
     1741 gtcactgatg gcacaagggg agaaaaaatg aaatttgaag tataccggga gatccatttt
     1801 tcttctgctc accgtttgag aaattattgc ggaaagtgtg aaaatattca cggacataac
     1861 tggcgcgtca gattatgtgt cacaagaaaa gaacttgata agacaggatt tgtcatggat
     1921 ttcaaggaca tagacagaat tttaaaaaag atcacagatt ttcttgatca caaggatctt
     1981 aacgctcttg agcagtttca tgaaataaat cctacggcag aaaatattgc gctgtttatt
     2041 ttcaacgaag cagaaaaaga ggttgcggcg gttgacagag aggcatccgt ttcaaaggtt
     2101 tatgtgtggg aaagcgaaag gtcatgtgcc tgtgtcgggg agtagaatga atttcaggaa
     2161 aaatgttgcg gcagtgatag ttgatgataa agggctgata ttagtgggta aaaggactga
     2221 tgttgcagat gcgtggcagc ttcctcaggg gggtgttgac aaagatgaaa atgaacagta
     2281 tgcagttttg agagaagttt ttgaagaaac gggaattcag ccgcattttc tcaatgttgt
     2341 gaaaaagacg agtgttatca gatatttgtt cccgcctgaa ataagaaagg gtatgaaatt
     2401 cgatggtcag gaacagatct attttctcat aaagatagtt ggcccgggct ggtcgcttga
     2461 aagatcagat gaattttccg attttgaatg gcttgatcag aatgaagttg taaaaagagc
     2521 tgttgaattt aaaaaaaata gttatattga agcgttccgg caattattcg gagtgaaaga
     2581 tggcatctga aaaaaagaaa aagaagtcga taataagaaa aatagctgaa ttgaagaaga
     2641 ccataatgat gctgatcctg atttcggcac tttccggttt tctcataagt gtcgctgttt
     2701 attacaaata taaggcggaa ataccttcta tcaacacagt cagcgactat aagcccgatg
     2761 caggcacaaa agtttattca tatgacggtg aacttattgc ggaattctat cacgagaata
     2821 gaagaataat tgtcccttac gacaagatac ccgaaaaact ccggcttgct ttcgttgcag
     2881 gggaggacag ctctttttac gagcacagcg gaatagatct tttcggaatg atgagggctg
     2941 gattcaaata tcttgtgact ggggtcaagc agggcggaag caccataacc cagcagcttg
     3001 ctaaatcttt tgtcggaact gaaaggacat ggggaagaaa gataaaggat ataattcttg
     3061 ccattgaact tgaaaaacat ctttcaaaag aagagatcct ttatctttac ctgaatgaaa
     3121 tatatctcgg gagcggttct ttcggcgttg aagcggcatc aagaaaatat ttcagcaaac
     3181 atgtgtggga acttgatctt catgaaattg ctgttcttgc agggcttcct aaatttccct
     3241 ctcttgcaaa ccccatacag aatcctaaaa cggctttaag aaggagaaat tatgttctga
     3301 aaaggatgtt tgaagaaaag tacataactg aggcggaata tgagaaagcc aagaacgaac
     3361 cggttgttac tgatcctcag aaagagcttt ttcttgataa atccccttat ttcagcgaaa
     3421 gggtgagacg atatcttctt gaaaaatacg ggtttgacaa actctacaag gaagggatgt
     3481 cagttcagac aaccgttgat atgcgggcga caaaacttgc ccacgaagct gttttcatgg
     3541 gattgcggga actttcaaaa aggcagggat atgcaagaag cactgaaaac agttatcaga
     3601 aaagcaagaa atttaaaggt gaagtgacaa acccgcttta caagataaat ctgaaaaagg
     3661 atcttgagaa atatctgctt agacatgagg agcatttcgg ggaagtaact gaaagcaaca
     3721 tcagcagcgg catatattat cagggcgttg tcattgaaat aaagcccaaa gaggcggtaa
     3781 ttcaggtcgg gaaagtgaaa agaccggtat atcttgaaga tctgatgtgg gcaagaccct
     3841 ggaatccgac aggttatccg ccggtgatga gggaaatgtc agatgttctt tcacccgggg
     3901 atgttgtaat ggtcagggct actgacaaag aagggacaag agatgtattc gatcatcgca
     3961 actattcaaa aaaactgaag aaagcatttt tctttgttct tgaacagata ccggcaagtc
     4021 aggcggcgat aattgtaaag gacccttatt cgggatatgt aaaagcgctg atgggcggat
     4081 acgattttga gataagtgaa tttgacagga caactcaggc atgcagacag ccgggaagtg
     4141 cgatcaagcc ggttttttat tcacttgcac ttgaaaaaca gggtggaaga ccaccgcttc
     4201 tgaccccggc gtcaatgata cttgatgctc ctgtaacagt tgtagataca aattttaagc
     4261 catctaattt tgaaaatacc ttcaaaggtg aaataactgt ctgggaggca ttgatacatt
     4321 cgatgaatac tccggcaata agggttttgc agaaaacagg tcttgccgat gcacttaaat
     4381 gggctcagaa tctcgggata aaaagccaga taaggcctga gtttggaagc atgctcgggg
     4441 cgagctgcct gacgatcgat gaactgactg atgcctatgc tcattttccg aacatgggaa
     4501 aaagtcctta cacgacatac atcagaaaag tttatgacaa atacggcaaa gttctggagg
     4561 acaatacggt cttttacgat ccctatattt cagctccgga aaagatcgca agactggtat
     4621 attttgcaaa aagggaggaa aatcaagttg ttacgcctca gactgcatat atagtcaaca
     4681 aagttcttga agatgtcgtc aaccgcggta cggccggatc agcaagaggg cttaacaact
     4741 ataaatgcaa taagaaacca aaccctgctg aagtgactga atcatctgaa caggttgagg
     4801 aaagttctta ctgcagccgt catgttgccg gaaagacagg cactacgaac gattattttg
     4861 atgcgtggtt cattggctac tcccccaatc ttgtcgcagg tgtctgggtc gggaacgatc
     4921 agcacggaaa accgatcgga agagttgaga ccggcggcaa ggcggcactt ccggtgtgga
     4981 tggatttcat ggggagatat ctcgcaaatt ctgaaccgac tgattataaa atcccttacg
     5041 gaattgtctc aagagcgatt gataccgcaa caggtcttgt aactgaatca ggcggcgtaa
     5101 gaatgtattt tcattcagga agtgagccgc agggaagtgt cgatgaaaag cacatccttg
     5161 atccgacaaa aggtccggag gggatatttt gagcacaaga tcgattctga aaagcagcta
     5221 tgtgcttgtc ggattcactc tcatttcaag atttttaggg cttgcaagag aatctgtcaa
     5281 ggcggcaatg ctgggcacca cagctttaag tgatgctttc tctgtcgctt ttctgatacc
     5341 taatcttctg agacggcttt ttgcagaagg gactatctct gccgctttca ttcccacttt
     5401 taaaggatat ctcagcagga atgataaaaa ggagaccgca gattttctga gcgctgtgtt
     5461 cactttcggg ataacagtct ttgctgtaac ggtatttatc ggtatagggc tgtctggtgc
     5521 tctcgtaaag atattcgggc cggctttttc tcaagatgtt tattctgaag cggtttttct
     5581 tacgcagatc atgtggccgt atcttgcctt catttctgtt gcggcactgc ttcagggtgt
     5641 tctgaacagt ctgggcatat tcggtccgag cggatttacc ccgattcttt tcaatctttc
     5701 gtttattaca gccgctttca ttctttctcc ttttactgca aatccggcaa gggcgatagc
     5761 gatcggtgtg actgcgggcg gaataattca gatgcttttt cagatcccgt tcatgatgaa
     5821 aacggggatg agattttcgt ttacaaatct gaaaaaggca ttttttcacc cgggtgttag
     5881 aaaagtcatg cggctcatat caccgacact tcttggaatg ggcgcatatc agatcaacat
     5941 tatcgcaacg acccatatag caaacagggc aggagaggga atagtgagct ctcttcagtt
     6001 ttcaaaccgg cttgaggagc ttatactcgg ggttttcatt gtttctctga gcacggtcat
     6061 cctgccggaa ctttcaactg atgccaaaaa aggggattgg gaaaatttca ataaaaatct
     6121 ggtgttcgga ataaaagtta ctgcgatgat atcgataccg gcaacagtat tcacgcttct
     6181 tatgagagaa gaaattgtat cccttctttt caaattcggc aggtttgatg atgaatcggt
     6241 gcggctgact tcctatgccc tgatgttcca tatttcaggc ctttttttca ttgctgtgac
     6301 aagaattctc tttcctgcct tttttgctca ggaggacaca gtgtcgccta ctgttgcagg
     6361 gattgtttca gtagttgtga acattgttgc ggcatatctt cttgtcggct cgatgaaagg
     6421 tggcgggatc gcatttgctt ccacaatatc ggcttttgta agcacagtga tacttgtctt
     6481 tatgctcaga aaaggtggaa agataaatat gaacgcagtt ctttcatcat tttcatatgc
     6541 gtttagattt ttattaatat cagtatgttg cggagtgccg ctctatttta caaaagattt
     6601 tgtatatggt gttttttcaa aaataccggc aatgtcagcg tttggtgaga aaattatcag
     6661 ggaatttgtc cctttttcta ttatcaccgc tatctttgga atatttcttg ttgcagtgct
     6721 tataatcatt aaggatgaaa acgcggttta tatttttgac aaggtcgcaa ggaggttaaa
     6781 atggaaaaag tgaagcttct catcatcggt gcaggtcccg gcggttatgt tgcggcaata
     6841 agggcggctc agctcggtct tgagccggtt gtaatagaaa aagccgatct tggcggaaca
     6901 tgcctgaact ggggatgtat tccgacaaaa gcattgatcg cagcagcgga aacatttgag
     6961 aacatcaatc attccgacgc attcggaata acggcaactg ccacatttga ctggacaaaa
     7021 gttgtggcac atgcaaagaa tgcagtgaca aaaaatagag caggagttca atatcttttc
     7081 aaaaagaaca aagtccgtct tgttactgca acagcaaagt tcataagccc gaaaactgtc
     7141 aaggctggcg aaactgagtt cgaagcagaa aatataataa ttgcaaccgg ctcaaaagtt
     7201 cgtgatgttg cgggcttccc ggtggatgga aaaaacataa taagctcaga tcatgccctt
     7261 tttcaggaaa aacttcccaa aagcattgca ataatcggcg gcggagtgat cggtgttgaa
     7321 atggcctata tttatcattc gttcggagtt gaagttacaa taattgaagc gatgaactcg
     7381 ataattcctt ttgaagacgc tgatatttca aaagagctca caaaagagtt caaaaagaaa
     7441 aaaatgaaaa tacacacagg cgtttttgtt gaaaaagttg agaaagtcaa tgatttaatg
     7501 gtgataacgc ttaataacgg cacaactgtt gaggcggcgc aggttctttc gagtgtcggc
     7561 agaagcccca atcttgacgg acttgacatt gaaaaaggag gcgtggtttt aaatgaaaaa
     7621 ggattcataa aaactgacga actttttaaa acaaatgtcg aaggtgtttt tgcgataggc
     7681 gactgcattc cgaccccgat gcttgcccat accgctgaac atgaagggat cgttgctgtt
     7741 gagcagattg ccggaatgaa agttcataaa attgactact tgcttaatcc gggatgcata
     7801 tactgtcttc cctcaatcgg ctcaatcggc tacagagaat caactgcaat cgaaaaaggg
     7861 ataaaaataa aaacaggcaa attcccgttc agtgcaaacg gaaaagctgt tgcatcaaat
     7921 cacacgaccg gttttgtaaa agtaataatt gaggaagaaa gtcacaaaat tatcggagca
     7981 catatcatcg gatacggtgc gacagacctc atttcagagt ttattctggc aatgaaactc
     8041 ggtgcgactg ctgaagatat tattgaagcg atccatcccc atccgacact ttgtgaagca
     8101 tcccttgaag cggtgctgaa ttcgcttgga agaactatcc acatttaacg gaatttatat
     8161 gtatcaactt ttaatgcttc cggcagggct tgcgtttttc cttttcggga tgacacagct
     8221 cagttcgatg ctttcgggtt tcacaggcaa gtcttttgca gacaagctcg acaaatggac
     8281 aaggaatgat tttacaaaga ttttcacagg ttttacaata acgacccttt ttcaatcttc
     8341 aggtgcaaca acggcgatcc ttgtttcgat gacctccgtc gggcttgtaa cggtcacgag
     8401 ttcagtaagt tacctgatcg gagcgaacat tggttcaata acaacagcgt ggatagttgc
     8461 agtaaaagtg acctcattcg gtgttttcat gtttaccagc gggtttttat taaaatttgt
     8521 gctgaaaaat gaaaagttaa aaaagatctc tatatttgtt gccggtctcg gtcttatatt
     8581 ttttggtctt caaatgatga tggattcagt ttctttttta aaggaaaatc aaaaagcatt
     8641 gtcatttttt tcgggatttg atgcatcttc atcatttatt tccatgctca ttcttgtgat
     8701 cgcaggtgtt gtttttactg ctgtgatcca ttcaagcggt gcgactgcgg caatgataat
     8761 cggactggtt tcgactggtg cagtctcttt gcatagcggt gcggctgtgg tgctcggttc
     8821 aactctcgga acaacggcta ctgcatatct tgcttcactt ggaacatcag ccgaagggaa
     8881 aaggacggcg tttttacaga tattcatcaa tgttattgaa ctcttggcag ggcttgtgtt
     8941 cttctatcct atggtggaac ttgtattgac gatctcaaaa acatattcat tcgatgccgg
     9001 atttatgata gctctttata tgactttcct taaaatcatt cttgccgtaa tagtttttcc
     9061 attaagaaaa gtgtttgccg taactgcaga aaaattcatc agaaagaaat tcagactgat
     9121 aactgagtcg ctcaatattt cacccgtaac ggctgacgat actcctgaaa aaattaagga
     9181 aaaatttgaa ccggtgatcg aactgtgtgt gaaatatata actgatatgc ttagttacag
     9241 ctatatcgga ataaggaaac cggcactgcg ttccatttat aaaaaagtta tccactatga
     9301 agagataatt gacgaagctc aaaaggatat agttgaaatt atcagcaaaa gcagaaacag
     9361 tcacactgat atactctggc tttttcttaa aatggctgat gaacttgaat caatgggcga
     9421 ccacgccaag tctatcgcaa aatatggagt ccgtcttgat gagatcaggc ataaactgaa
     9481 cgatgagcag aaaaagatcc ttcttgaatg ttatctgatg gttttcagac agttccatga
     9541 agtgtgtgta aaaaagaact ttgactgcgc ccttgttgca aaaggggaag agatagaaag
     9601 acatttgagg cgtgaaaaaa gaaaaatata ttcccttctt tgcactgaat cggatcacaa
     9661 ctacgaaaag cggctcatac ttgttgatat actttctgaa tacagcaaat tcaaccattc
     9721 ggtaaaaagg atccttcagg taaacattga tgtgagcgaa ggaaaaggga tatatctgtg
     9781 ggaaagcagg tgtaaaaatt aaatgacatt cacagaaagg acagatttgg tattcggtgc
     9841 agacggaatt gaaaaactca aaaattcaac cgttgcggtc tatggtcttg gcggagtcgg
     9901 agcaagtgcc gctgtatccc ttgtcagagc aggggtcggc acaatagcag tcaccgattt
     9961 tgataaagtg aacatttcaa acctcaaccg tcttgttttt gggtttgaat caacgctaaa
    10021 cctcgataag accgaggttt tcgttaaaac agccaaagac ataaaccctg acataaaaat
    10081 tataatatca aaagatttcg ttcacggagc tgatgccgct caaaaagttc attctgccga
    10141 ttttcatatt gatgcaattg attcactcaa cccgaaagta aacctgatta tcgcacttct
    10201 tgaaaacaat gccgccttca tctcctcaat gggaacaggg ggaaaattca gaccggagtt
    10261 acttcagatc tctgacatct ggaaaacgga tgtttgccct ttatcaaaat ttgtaagaaa
    10321 acggctcagg aacagaggaa tcagaaaggg atttcccgtt gttttcagca aggaactttc
    10381 cacagaaccc gttgaccttg aagacgacca caatgaagaa atgcccggca gaaaacggct
    10441 cactcagggc tccacaccct ttgtccctca agccgcagga ctcatccttg caagttatgc
    10501 agtaagaaaa ctgctgggga aggggtgaaa agaattgaaa aaggggtttt ttgacagtgt
    10561 gttccggaga gtatatatta tttggaattg ggaattagag gacggataaa caatatgaga
    10621 aaagataaaa aaaaagaatc gaatcaatca caaattatcc tttatactac ccctgacggt
    10681 gatataaaaa ttgatactat tttgcaggat gaaactatct ggcttaacca aagtgcaata
    10741 tcagagcttt ttgatgttaa aattccagca gtatccaagc acttgaaaaa catttttgaa
    10801 agtggggagc tggatgaaaa agtggttgtt tccattttgg aaaacaccac taaacacggt
    10861 gccattgaag ggaaaaccca aaaaaaagaa gtgaaatact acaaccttga tgttgttatt
    10921 gcggtgggtt atcgggtcaa ctccaaacgg gcgactcagt ttagaatctg ggccgcaagc
    10981 attcttaaag aatacatttt aaaaggcttt gttatggacg atgaccgact gaagaatttg
    11041 ggcggtggcg gctattggaa agaattgcta cagcggatac gcgatattcg tgcatccgag
    11101 aaagtatttt atcgccaggt tctggatata tacgcaacca gtattgatta cgacccaaaa
    11161 gcagaggttt ccatagaatt ttttaaaaaa gtgcaaaaca aaatacatta tgcagtccac
    11221 ggacaaacag cagctgaagt tattttcaat cgtgccgatt ctgaaaaaga atttatgggt
    11281 ctgatgtcgt ttccaggcag ccgtccttat ttaaaagatg tggttgttgc aaaaaattat
    11341 ttaaacgaaa aagaacttcg ttcgctcgga cagattgtat cagggtattt ggattttgct
    11401 gaacggcaag ccgaacggga acaagcaatg actatgaaag attgggctga acatttggat
    11461 aaaattttga ccatgagcgg cgaaaaacta ttgctgggag ccggttctgt aagccacgaa
    11521 acagcaacag aaaaagccat ctcagaatat aaaaaatatc agcagaaaac attgagcgaa
    11581 gctgaaagaa attatctgga aagcttgaaa acattagaaa gtaaaagcaa aaatcaaaaa
    11641 taaattgata ttcacccctc acctcggctt cccagacctg tccaccgttc acccatttgt
    11701 acaaaagtcg gggcaaattt gattatattt aaggaaaaat gccaaaactg gcaagatttt
    11761 ttgccggatc cgaaaatgat tttaactatt tcccggcgag ttttgctttg atgtattcgt
    11821 cgagtccgcc gcactgcatg atctcgtcaa cttctggcgg aaggggtaaa ttatcggtcg
    11881 tcagagagtt ccgtatttat tccacatctt ctttatggtg tcaagccact tgtcccttaa
    11941 ttctttcgga gcgacgatct cgctgtccgg ggcgtaggcc attgttcttg caatgatctc
    12001 gttgtgtctt tctccaacgg gtaaagttat ttcaatataa ttgatgcctt tagatgatcc
    12061 ttctataact atctgatctt tatgccattt ctgatttctg atataatgca ttgacggttc
    12121 agatattctg atcgttgcga gtgcggtctt tgatgatttg aacattccga atgagccgtc
    12181 tgtgtaattt ttgagctctt tttttgtgac tgcgttttca aacatgtttt cagttacccg
    12241 gcaatttgtt atccttgata tgagaaagac cgcaagctcc ttccgtttgt ggcagtatcc
    12301 tatgatatac cattttccaa gatagtttgt cagaagcatc ggctcaacaa ctcttgatga
    12361 aatttcccct tttgcgttgc agtaaccgat ctcaagctgt aagctgctct ttatggatcg
    12421 gagtatgttt ctgaaatccc tgatatccat gatgtcctga tcagatgaat cataaacgac
    12481 gctgtcaagg atgttttcat attcacttgg cacataactc tttatttttg ttaagatatc
    12541 ttttgaaata accggtacat agggaactcc gccaagacgg aatccgtcag ttatccggtg
    12601 aacgaagata taatacagag cggcatcttc agcggctgaa aacacagttt caaacggtgt
    12661 ggagtaggtg tacccatgaa atttgtttga atattcaagc ggagcgttta gaaaaagccg
    12721 catatactcg atgtcccgct taaccgtgtc aatatggact tcaaattctt cagcgacctc
    12781 ttttcttgtg acaaatcccc ttgatctgat ccgttcatca atgaatgtta tccttgacag
    12841 gtttgacatt ttatcctccc gaacatctaa ccgggatgat accatcaggg ctcaatgatt
    12901 tcaacttctt ccgtgacatc ttccaaacta tcaaccgttt cactgtcctc ttcaccaaat
    12961 gatgatgccg cctttgatat atcctcaatt ccttcggcaa tgatctcaag cccttcttcg
    13021 atccgttctt tgaaagatgc cccgaaaaga tgtacttcgt cagcatcaaa accgaatatc
    13081 cttttaaata gattaagcat cgctaccctc cgctgttaaa agttctgtct ttttttcatc
    13141 ttttatgatg atccccattt ttttgaacac tgatgaaact cccgcttcaa aaacattgtc
    13201 tagggctgaa actgcaaaac cgggatattc atcaagaccc ataattttcc ccctggtaag
    13261 gagaagatct ccgagaccct tgaaaataag ccgttcttca aaaccgtaag tggttataac
    13321 cattgggagc tctttcaacc gccagaaagg atcattcccg agcacaagat caacaggctc
    13381 aaccccatgt tctttcagga agtcacacat ggcaacctca agagaattta caaatctttc
    13441 gaccactccg agagcatgat ccctgagttt agtgatctca ccttcaagaa ttccgttaac
    13501 cctgtcggtc atttcatcaa tttcatcgcc atcacttttg atcacatagc ttttcttcac
    13561 aaccgggaac ctgctttgtg aaagggcatt cttcgcctca tgataacccg acatcatctt
    13621 aagtctggcc cgtaattcct gcggaaccgg taagtttttc ttcatcgtct cctcctgaaa
    13681 aaaacattaa tgaca
//
LOCUS       DAOOYB010000055        13619 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_4575_length_13619_cov_12.974639, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000055 DAOOYB010000000
VERSION     DAOOYB010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13619
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4575_length_13619_cov_12.974639"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2897
                     /gene="rpoC"
                     /locus_tag="PL056_04875"
     CDS             <1..2897
                     /gene="rpoC"
                     /locus_tag="PL056_04875"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HPM46380.1"
                     /translation="VLLNRAPTLHRLGIQGFEAVLVDSKAIRLHPLVCVAFNADFDGD
                     QMAVHLPISIEAQLESRVLMMSTNNVLSPASGTPVITPSQDMVLGLYYLTTAKPKSKG
                     ENKVFFSDVEALMARDAGIVEWQSMVRVRIDGKIYVTTPGRLLIWKVAPKVLGFETIN
                     KQMKKSDWSNILDRSFRVAGGKATVIFADKIKDLGFHYSTKAAYSISVDDIKVPEAKK
                     TIIAETEKEVREIEEQYNLGVVTKSERYNKIVDLWDKANERVTNKMMDELIEQTINHP
                     IWGKVKMPSENPIYVMADSKARGSKDQIRQLGGMRGLMAKPSGEIIETPILANFREGL
                     DSLDYFNSTHGARKGSADTALKTANSGYLTRRLVDVAQDVVVVEEDCGTNTGIWAEAL
                     MNDSGEVISPLRERILGRVSLDDIEDPDTGEIILEANELIDEATADRIVELGIDSIMI
                     RTVLSCRSKYGVCAKCYGRDLARGHLINVGEAIGIIAAQSIGEPGTQLTMRTFHVGGV
                     ASRTIEKSSHETKRAGKIIWKDIKTLEKLDGGFVAMNRNGKLLIVDDKGRERERYPVT
                     YGTNLRVANGAKVNPGDILADWDPYNTPIIAETDGVIAYEDIENGVTMKEEVEAQTGR
                     SKAVVIMTRDAKMSPKLLITEEGKKGRKKAKTIARGEAIYHLPANAYIEVANGDTVAT
                     GDVLAKVPRAARKTKDITGGLPRVSDLFEARKTKIPAKVSEISGYVSVQSEGKGARSK
                     RIITITPEFGETTPPIPVPKERHINVHDGDYVEAGEPLIDGSVDPHDILRIKGEIEVA
                     KFLVQEVQRVYKLQGVPINDKHIEVIVKQMLKKRKIVESGDTTFMKGEFIEKEKLESV
                     NETIEKKGLKPATWETILLGITKASLNTESFFSAASFQETTKVLTNASIEKKKDVLRG
                     IKENIIIGKMIPAGTGFPKYHDSNYEISFPENEKMEEESHEADPEETKPE"
     gene            3012..3377
                     /locus_tag="PL056_04880"
     CDS             3012..3377
                     /locus_tag="PL056_04880"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPM46381.1"
                     /translation="MTKKAKILIVDDDLELLSTMSRYLELKGLEVFGKTSGEEAAELM
                     AKENFDLIILDIVLPGINGIEILKSIKKKDPQQKVIVMTAMLSIENTIAALREGAVEY
                     LLKPFSSMEYIWTVIEDYL"
     gene            complement(3358..4392)
                     /gene="plsX"
                     /locus_tag="PL056_04885"
     CDS             complement(3358..4392)
                     /gene="plsX"
                     /locus_tag="PL056_04885"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HPM46382.1"
                     /translation="MSSTIIVDAMGGDSPLDVPVQSSLEVCKENPDIKLILVGSEKDI
                     IDSIDEKIPENISIVNAESEITMDDSPAKAYREKIDSSIHKGIDLLVNGKGDAFFSAG
                     NTGAIVAVSYFKSGVIQQVSRPALATVYPSVDSKKLIILDLGATLEPKPENMYDFGVL
                     GEAYRFCITGLEDSRISILNVGEEDTKGTRTILEAAEMLKRSPLNFAGFIEGNRLFVK
                     PDTDVVVTDAFTGNITLKTVEGLNETISRLIKSRIAPPQFKRTRTFIFNKIFGEVFNQ
                     FQYNLYGAALLLGMNHLIGIGHGRSDKKAFKNAILRLHKCVERDFLRKFKERTIHIKN
                     SSGRIKDNLQ"
     gene            complement(4401..4586)
                     /gene="rpmF"
                     /locus_tag="PL056_04890"
     CDS             complement(4401..4586)
                     /gene="rpmF"
                     /locus_tag="PL056_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HPM46383.1"
                     /translation="MAVPKRKHCKARSRKRRGGHLKSDPVATISCPKCGGTMMPHRIC
                     PNCGNYKGKEFVSVEEQ"
     gene            complement(4615..5052)
                     /locus_tag="PL056_04895"
     CDS             complement(4615..5052)
                     /locus_tag="PL056_04895"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HPM46384.1"
                     /translation="MKLFLSEITDSVNSRKVEFVPVGGELGERTRVTGVDMNLDFYRA
                     GETICLKFTGVYDVETTCDRCSAGIEMSSDIEESYYVFPQAAGDEVDYHYSGDSVEID
                     DFIREILVMNMPNKILCSDECKGLCSVCGANLNDVDCKCSSGE"
     gene            5216..5965
                     /gene="map"
                     /locus_tag="PL056_04900"
     CDS             5216..5965
                     /gene="map"
                     /locus_tag="PL056_04900"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007428495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HPM46385.1"
                     /translation="MSGIIIKSPEQIEGIREAGKILSELYLELKNYIKPGVSTLQIDS
                     FADRFIKKRGGYPTFKTVAGYRHATCVAINNEVVHGIPKKTKIVQRGDLISVDCGVTL
                     NKFIGDSTVSYLMPGASETAKKLFDVTKKCLDIGIQECFPGKRVGDIGAAIQEYAESN
                     GFSVVTEYVGHGTGVLLHEEPSIPHFGTRNTGTLLQEGMVLAIEPMINEGTYKTRTLN
                     DGWTVVTLDGKLSCQFEHTVAVTKNGPVVLT"
     gene            5985..6278
                     /locus_tag="PL056_04905"
     CDS             5985..6278
                     /locus_tag="PL056_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46386.1"
                     /translation="MVKHIIYIFIILFLLANFCWEKKPLWKHFIPVAEDAMENVVEGT
                     EKSLKEGKNIIKEGAEKIKETIKDAPKAEISEEDREKLNEIIKKSSEKDKKKK"
     gene            6309..8153
                     /locus_tag="PL056_04910"
     CDS             6309..8153
                     /locus_tag="PL056_04910"
                     /inference="COORDINATES: protein motif:HMM:NF025067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HPM46387.1"
                     /translation="MKIVKYFFQDIPEKGEKAFKRFYLPLTVSMVSTFFVIFLLYEEK
                     FFSGRFYSVENMAMALYLGVPVLLTTFLVAEYYKLNQMLKCIFGLSILAAVIFYYFSL
                     PEKFLVTDTIRFILLSVFSFMTLFSFPIFLKKDRFVTRKIALFLYQRMMFAVIVAVLV
                     YAGLSFAVYAIQLLFIPGWNDSFRLTSAIFAVSSGIIAPWVVMAGIPEIEKISEVEVV
                     EKAAALISKFVFIPVVLFYLVILYLYFGKVLFFEELPKGMTSYLILSFCTAGSITYSI
                     ILEESKRAQKGITAMYEKWFYRTVFPLLFLLWYAIWKRTDDYGITEKRYILIVLSILL
                     TTWASYFIFSKRKNIVIIPLLTSIVSIFISFGPWGMFETSYRSQKTRIIEILEKNLLL
                     ENGKIIPSTKDVSFEDRKNLGSALLYIETRWGLNRISEIIPENANISTTPQKYLSRYS
                     GESSNTFKLMKWMGLKYVREWDNVENSESFSFYLNPRNGYAIKGPFDAMVSGTCPTYE
                     HEKKEGTPKSGIRTEFDKECKVLKIMNDNGMIFELDLLPVIKKMNEKTDKTNYNLSVD
                     QLSHNYENDSVTATVLITQAHGRLKSEKENSTISNISVSVYYFLKIQE"
     gene            8163..8957
                     /locus_tag="PL056_04915"
     CDS             8163..8957
                     /locus_tag="PL056_04915"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HPM46388.1"
                     /translation="MSGFILRSDELKKNFKDYIILDCRENPADCKHIAGSYFVDGESV
                     LTGKKGQHGGRHPFPNMDEFVSDMENLGISDSSKVACFGDFGARAAFMLRLFGITASF
                     ISGNSETLKRSGIPFDNDDPAPVKKGKITGKINNDLLVSMEKVRKNINNTEHILIDSR
                     SGERYRGENEPLDPIAGRIPNAVNFFWQELIKPDGTFLSDEEIRKHFNIDTGKNITLY
                     CGSGVTACFNWLALRQIGVKCSIYTGSWSDWISYPENLDRIEPKIH"
     gene            complement(9399..9755)
                     /locus_tag="PL056_04920"
     CDS             complement(9399..9755)
                     /locus_tag="PL056_04920"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HPM46389.1"
                     /translation="MLTKERTYFRKHARYSRHYYDMFMLSRSVIRESALKDIELLESV
                     TAFKKRFYPRGWARYDLAVPGTMKLIPPDHVLKFMKNDYRDMQNMIFGEYPSFENIIN
                     GLSMLEGEINSIKASR"
     gene            complement(9781..10422)
                     /locus_tag="PL056_04925"
     CDS             complement(9781..10422)
                     /locus_tag="PL056_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018284811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6088 family protein"
                     /protein_id="HPM46390.1"
                     /translation="MNIIPYRNCLELNMNTLKNKIVSRIYGKGRGWCFTRSDFLDIAS
                     PEAIDVTLHRLNGDGMIARALRGVYCYPKFNKLLNIFLSPDIDQVAQAIAGKFKWEIQ
                     PTGDTALNILGLSNQVQGKFVYLSTGPSKKYKIGNTDLIFKRTALKNSGFRHRKSSIV
                     FQALKALGEENVSPDTIDSVRDFLTGDEQRKVLKDTEKTTAWVYEIIKKICKD"
     gene            complement(10489..11040)
                     /locus_tag="PL056_04930"
     CDS             complement(10489..11040)
                     /locus_tag="PL056_04930"
                     /inference="COORDINATES: protein motif:HMM:NF027338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HPM46391.1"
                     /translation="MAKYFLKQGWVKKPIENLKVECDLNISTDSISAVFNVTEPTVRA
                     VNTGFNQPVWQDSCVELFLSFDNKNYYNFEINCIGSILAQYGENRHNRKFINPQALEL
                     IKVSSTLGNKPFGVKESVTSYQVKVFIPKEVLIFDENPDLINVSANIYKCADLSPTPH
                     YMYLFEIKTEKPDFHRPEFFRGF"
     gene            complement(11103..11453)
                     /locus_tag="PL056_04935"
     CDS             complement(11103..11453)
                     /locus_tag="PL056_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HPM46392.1"
                     /translation="MKVAKLMISLIALMFIFSGFNLFAQDEPAPEKEMKKEQIQEHKE
                     QKEQKKEMKQEKKQEKKQEKKQEHKEAKEENKEMKKEMKQEMKQEKKEQKKEQKKMNK
                     EGKEGGEKKGDCPK"
     gene            complement(11545..>11771)
                     /locus_tag="PL056_04940"
     CDS             complement(11545..>11771)
                     /locus_tag="PL056_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46393.1"
                     /translation="YFFESDEWSGIKKKRTDHAIAPSFKIVINATDFLAIVFGYKYLK
                     NFSTLGESDSDYYDYNYDRHRVMLEFSFKF"
     assembly_gap    11772..11871
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11872..12162)
                     /locus_tag="PL056_04945"
     CDS             complement(<11872..12162)
                     /locus_tag="PL056_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46394.1"
                     /translation="MKRIKNETKNGKNRAGSILKTLVILLIILCFQTLAAIEDGGGSV
                     SIGSSYDSNPGDDETSTTRGGRKDLLKDVGDFSLDLSTDLFIYPVQQLYLDYF"
     gene            complement(12113..12388)
                     /locus_tag="PL056_04950"
     CDS             complement(12113..12388)
                     /locus_tag="PL056_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46395.1"
                     /translation="MLKELFALIFIFFFAMTPLTGEEKKEEPKKPKVKTGVEVEKGDN
                     APKWEDEILKKKKEAKKESEKEMKKEKREKKLEKNKERNEKRKKSGR"
     gene            complement(12381..12956)
                     /locus_tag="PL056_04955"
     CDS             complement(12381..12956)
                     /locus_tag="PL056_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46396.1"
                     /translation="MEKQSPEKIADALFEQLSYKKKKLPENFALEMASKFSAVSTRKR
                     TGSLFMKSGFAVAAVIIIFASGFFAGTIVNENNGIEEYFEHSVIASAEVLTGETVTIK
                     LVYDSENDVDDVDFSINLPKGLAFNSENPEILNTKTINWSGALKKGRNEIPFVVKAVE
                     AGLWHIDATAEYDGGSLNHEIVVKATGEINA"
     gene            complement(12991..>13619)
                     /locus_tag="PL056_04960"
     CDS             complement(12991..>13619)
                     /locus_tag="PL056_04960"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPM46397.1"
                     /translation="VYFGTNLIYTLSNLELCFGELMGIQEKYFIHRSKSGDGGSFEKL
                     VKKHYQVLFRFALSITKGNEDLASDVLQNALLKAFLNISSFEGRSSFTSWLWVIIRNE
                     FISLLRKESGEITGTEGDISDDQPDTESVSIENSLMLEQRKENLLRMIDQLPENYREI
                     IMMIEMSGMSYVEASEFLGVPVGSIKSRLSRAREELHRLIERNLELFS"
BASE COUNT         4180 a         2759 c         2537 g         4043 t
ORIGIN      
        1 ctgttcttct taaccgtgcc cctacacttc accgtcttgg tattcaaggg tttgaagcag
       61 ttcttgtcga ctcaaaggcc attcggcttc acccccttgt ctgtgtcgct ttcaatgcag
      121 actttgacgg tgaccagatg gcggtacatc ttcctataag cattgaagca cagcttgaga
      181 gccgtgttct gatgatgtct acaaacaatg ttctttctcc ggcatccgga actccggtaa
      241 taacaccttc tcaggatatg gttctgggtc tttactacct tacaacggca aaaccgaaat
      301 ctaaaggcga aaacaaagtg ttcttctcag atgttgaagc tttaatggct agagatgcgg
      361 gaatagttga atggcagtct atggtcaggg tcagaattga cggtaaaata tatgttacaa
      421 ccccgggaag gcttctgata tggaaggtcg caccgaaagt cctcgggttt gaaaccataa
      481 ataagcagat gaaaaaaagc gactggtcaa acattcttga cagaagtttc cgtgtagcgg
      541 gaggaaaggc gaccgtaatt tttgcagaca agataaaaga tctcggtttc cattattcta
      601 caaaagcggc atactccatc tctgttgacg atataaaagt tcctgaagcg aaaaaaacca
      661 tcattgctga aacagaaaaa gaggtccgcg agatagagga acagtataac ctcggtgtcg
      721 taactaaatc tgaaaggtat aacaaaatag tagacctctg ggataaggca aacgaaagag
      781 taaccaataa aatgatggac gaactgattg agcagaccat caatcatcct atctggggca
      841 aagtgaaaat gccttctgaa aatccgatct atgtcatggc cgactcaaag gcaaggggat
      901 caaaagatca gatcaggcag ctcggtggaa tgagaggtct tatggctaag cccagcggtg
      961 agatcataga aacccctatt cttgcaaact tcagagaagg tcttgattct cttgactatt
     1021 tcaactcaac acacggcgca agaaaaggtt cagccgacac agcccttaaa acggcaaact
     1081 ccggatatct cacaaggcga cttgttgatg tcgctcagga tgttgtcgtt gttgaagaag
     1141 attgcggaac aaataccggc atatgggcgg aagcgcttat gaacgattcc ggagaggtca
     1201 tttcaccttt aagggaaaga attctcggca gagtttctct tgatgatata gaagaccctg
     1261 atacaggtga aataattctc gaagcgaacg aacttattga tgaagcgact gctgacagaa
     1321 tagttgagct cggtattgat tcaataatga taagaactgt gctttcctgc agaagtaaat
     1381 acggagtctg tgcaaaatgt tacggaagag atcttgcaag aggacatctt ataaatgttg
     1441 gagaagccat cggcattatt gctgctcaat cgatcggtga acccggaact cagcttacaa
     1501 tgagaacttt ccatgtcggc ggtgttgcat caagaacgat agaaaaatcc agtcacgaaa
     1561 caaaaagggc cggcaaaata atatggaaag atattaagac acttgaaaaa cttgacggcg
     1621 gctttgttgc aatgaacaga aacggaaaac ttctgatcgt tgacgacaaa gggagagaaa
     1681 gagaaagata tcctgtcaca tacggaacaa atctccgtgt tgcaaacgga gcaaaagtca
     1741 atcccggcga catactcgca gactgggatc cttacaacac tccgatcatt gctgagaccg
     1801 acggtgtcat tgcttatgaa gacattgaaa acggtgtaac aatgaaagaa gaggttgaag
     1861 cccagacagg tagatctaaa gccgtagtca tcatgacaag agatgccaaa atgtctccga
     1921 aactccttat caccgaagaa ggcaagaaag gaagaaagaa agcaaagacc atagcaagag
     1981 gtgaagcaat ataccatctt ccggcaaacg catacataga agttgcaaac ggagatactg
     2041 tggcaaccgg tgatgttctt gcaaaagttc caagagcggc aagaaagact aaagacatca
     2101 caggtggtct tccaagggtc agcgaccttt tcgaagcaag aaaaaccaaa attcccgcaa
     2161 aggtttctga aatttcaggt tatgtttcag tgcagtcgga aggaaagggt gcgagaagca
     2221 agcgaataat taccatcacc cctgagttcg gcgaaacgac accacctatt ccggtaccga
     2281 aggaaagaca cataaatgtc catgacggtg actatgtgga agcaggtgag ccgcttattg
     2341 acggatctgt agatccgcat gacatcctcc gcatcaaggg agaaattgaa gttgcaaaat
     2401 tccttgttca ggaagttcag agagtttaca aacttcaggg tgttcctata aacgacaaac
     2461 acattgaggt cattgtcaaa cagatgctca agaaaagaaa gatcgtcgaa tcaggcgaca
     2521 ccacttttat gaaaggcgag ttcattgaaa aggaaaaact tgaatctgtc aacgaaacta
     2581 ttgaaaagaa gggtttgaag ccagcaacat gggaaacaat tcttcttgga ataacaaaag
     2641 cttctcttaa cacagaaagc ttcttctcgg cagcaagctt ccaggaaacc acaaaagttc
     2701 ttaccaacgc aagtatcgaa aaaaagaaag atgttctccg tggaataaaa gagaacataa
     2761 taattggaaa aatgatacct gccggaacag gattcccgaa ataccacgat tcaaattatg
     2821 aaataagttt cccggaaaat gaaaagatgg aagaagaaag tcatgaagct gacccggaag
     2881 agacgaaacc tgaataaatt ctttatcaaa taacccattc tgttacaaaa agcacacatc
     2941 aataaattga caaatttttt ttatgaacta taatttatca atgatattga ttgtttccaa
     3001 ggaggcggta agtgacaaaa aaagcaaaga tcttgattgt tgatgacgat ctcgagctcc
     3061 tttcaaccat gtccagatat cttgagctga aaggtctgga ggttttcggc aaaacaagtg
     3121 gcgaagaagc tgctgagctt atggcaaaag aaaatttcga ccttatcata cttgacattg
     3181 ttcttcccgg cattaacggg atcgagatcc ttaaatcaat aaaaaagaaa gatcctcagc
     3241 agaaagtcat tgtaatgaca gcaatgctct ctattgaaaa cactatcgcc gctttaaggg
     3301 aaggtgctgt ggaatatctg cttaagcctt tttcaagtat ggaatatatc tggactgtta
     3361 ttgaagatta tctttgatgc gaccgctact gttcttaatg tgaatcgttc tctccttaaa
     3421 ttttcttaag aaatctcttt cgacacattt atgaagtcgc aatattgcat tcttaaaagc
     3481 ttttttgtcg cttcttccgt gtccaatccc tatgaggtgg ttcattccaa gaagaagtgc
     3541 cgcgccataa agattatact ggaactgatt aaacacttcc ccgaatatct tgttaaaaat
     3601 aaaagttctt gtccttttaa actgtggcgg cgcaattctt gattttataa gtctgcttat
     3661 cgtttcgttg aggccttcaa ctgttttcag agttatgttg cccgtaaaag catctgttac
     3721 gacaacatcg gtatctggct ttacaaaaag ccggtttccc tcaatgaacc cggcaaaatt
     3781 aagaggggat cttttgagca tttcagccgc ttccaatatt gttctggttc cttttgtatc
     3841 ctcctctccg acattaagta ttgagatcct ggaatcctca agacctgtta tgcaaaacct
     3901 gtaagcttca cccagaactc cgaaatcata catgttctcg ggcttaggtt caagagtcgc
     3961 accaaggtca aggattatca gcttctttga atcgaccgaa ggatataccg ttgcgagagc
     4021 cggccttgag acctgctgaa tgactccgct tttaaaataa cttactgcaa caatggctcc
     4081 ggtatttcct gcagaaaaga atgcatcgcc tttcccgttg acaagaagat cgatcccttt
     4141 atgtatggaa gaatcaattt tttctctgta agcttttgcg ggagaatcat ccattgttat
     4201 ttcgctttca gcgttgacaa tcgaaatatt ttcagggatt ttttcatcta tactgtcaat
     4261 aatatccttt tcagagccga caagtatcaa tttgatatca ggattttcct tgcagacttc
     4321 cagagatgat tgaacaggaa catcaagagg gctgtcccct cccatagcat caacaatgat
     4381 agttgatgac atttatttgc ttactgctct tctacagaaa cgaactcttt acccttgtag
     4441 ttgccgcaat tggggcagat acggtgaggc atcattgttc caccgcattt cgggcaggag
     4501 atcgttgcca ccggatcaga cttaagatgt ccgcctcttc tttttcttga gcgggctttg
     4561 caatgttttc tttttggaac agccatttaa tcctccacaa atataaaaag tcctttattc
     4621 accgcttgaa cacttgcagt caacatcgtt aaggtttgct ccgcagacag aacacaaccc
     4681 tttacattcg tcactgcaaa gtattttatt cggcatattc ataacaagta tttctctaat
     4741 gaaatcatca atttccacac tgtcaccact ataatggtaa tcaacttcat cacctgcagc
     4801 ttgaggaaaa acataatagg attcctcaat atccgaagac atttctatcc ctgcagagca
     4861 gcgatcacag gttgtttcaa catcgtaaac acctgtaaat tttaagcaaa ttgtttcacc
     4921 agcacgataa aaatcaagat tcatatcaac tccggtaacc cttgtccttt ccccgagctc
     4981 accacctaca ggcacaaatt cgactttcct tgaatttact gaatctgtta tttcgctcag
     5041 gaacaatttc atcaccgcaa atccgtcaaa aaaacaacac gggaaatata cactcaaaag
     5101 ccgttttgtc aagataatat aaaggaataa aaggagatga aaactaaaat tgattgtgtc
     5161 acagtaatga atattattac cgtagctgat attagttttt tgaacgggag agaacatgtc
     5221 cggcataata attaaatcgc ctgaacagat agaaggcatc cgcgaagcag gaaaaatact
     5281 ttcagagctt tatctggaac tcaagaatta tatcaaacca ggcgtttcaa ctttgcagat
     5341 agacagtttt gcagacagat tcataaaaaa aaggggagga tatcctacat ttaaaactgt
     5401 tgccggatac cgtcatgcca cttgtgtagc aattaacaat gaagttgtac acggaatccc
     5461 gaaaaaaacg aagatcgttc aaagaggaga ccttatctcg gttgactgcg gtgttacgct
     5521 taataaattt attggcgaca gcaccgtttc atatttaatg ccgggagcaa gcgaaactgc
     5581 aaaaaaactg ttcgatgtca caaaaaaatg tctcgatatc gggattcagg aatgttttcc
     5641 gggaaaaaga gttggagata tcggggcggc aattcaggaa tatgccgaaa gcaacggttt
     5701 ttcagttgta accgaatatg tcggacacgg aactggagtt ctcctgcacg aagaaccgtc
     5761 aatacctcac ttcggcacaa ggaataccgg cactcttctt caggagggaa tggtccttgc
     5821 aatagagccg atgataaatg aaggaactta caaaacacgg acattgaatg acggatggac
     5881 tgtagttaca cttgacggaa agctgagctg tcagtttgag catacggttg cagttacaaa
     5941 gaacggacct gtagttttaa cataaatttt ttcaggagga caaaatggtc aaacatatca
     6001 tatacatatt cattatcctt tttcttcttg ccaatttctg ctgggaaaag aaaccacttt
     6061 ggaaacattt catccctgtg gcggaagatg caatggaaaa tgtggttgaa ggaacagaaa
     6121 aatcactgaa agaagggaaa aacatcataa aggaaggtgc tgaaaagatt aaagaaacca
     6181 ttaaagatgc gcccaaagcc gagatatctg aagaagacag ggaaaaactc aacgaaataa
     6241 tcaaaaaaag ttctgaaaaa gataaaaaga agaaataatc aggttccgta atttaaaacg
     6301 gaggcaaaat gaagatcgtg aagtattttt ttcaggacat tcctgaaaaa ggggaaaaag
     6361 catttaaaag attttacctt ccgctaactg tttccatggt ctctactttt tttgtaatat
     6421 ttctgcttta tgaagaaaag ttcttttccg gaaggtttta ctctgtagaa aacatggcaa
     6481 tggctctcta tcttggtgtc cctgtgctgc tcaccacttt tcttgttgct gaatactaca
     6541 aattaaatca aatgcttaag tgcatttttg ggctgtctat ccttgctgcg gtcatttttt
     6601 attacttttc tcttcctgag aaatttctcg ttactgacac cattcgcttc attcttcttt
     6661 cggtgttttc atttatgact ttgttctcat ttccaatatt tctaaaaaaa gacaggtttg
     6721 taaccagaaa gatcgccctt tttctttatc agagaatgat gtttgcggtg attgtagcag
     6781 ttttggttta tgcgggactc tcgtttgccg tttatgccat acagctgctt ttcattcccg
     6841 gctggaacga ttctttccgg cttacaagtg caatatttgc agtaagttcg gggataatcg
     6901 ctccatgggt tgtcatggct ggaattcctg aaattgaaaa aatatctgaa gttgaagtcg
     6961 ttgaaaaagc ggcggctctg atttcaaagt tcgtttttat tcctgtcgtt cttttctatc
     7021 ttgtaattct ctatctttac tttgggaaag tgctcttttt tgaagaacta ccgaaaggta
     7081 tgacaagcta tctgatcctc tctttctgca ccgccgggag tataacatat tcaataatcc
     7141 ttgaagaatc taaaagagct caaaagggaa taaccgccat gtatgagaaa tggttttacc
     7201 gaactgtttt tcctcttctt tttcttctgt ggtatgctat ctggaaaagg actgacgatt
     7261 acggaattac tgaaaaaaga tatatactta tagttctttc aatattgctt acgacctggg
     7321 cttcctactt cattttctcc aaaagaaaaa acattgtcat cattcctctt cttacttcga
     7381 ttgtatcaat tttcatttcg tttggtccat ggggaatgtt tgaaacatca tatagaagtc
     7441 agaaaacgag aataattgaa attcttgaaa aaaatctact acttgaaaat ggaaagatta
     7501 ttccttcaac taaagatgtc tcttttgaag acagaaaaaa tctcggctca gcacttttgt
     7561 acatcgaaac aagatggggg cttaacagga tttctgagat aattcctgaa aacgcgaata
     7621 tcagcacaac tcctcagaaa tatctatccc gttacagtgg agaaagttca aacacattca
     7681 agttgatgaa atggatgggg cttaaatatg tcagagaatg ggacaatgtt gaaaattctg
     7741 aaagtttcag tttttatctt aatccaagga acggatacgc aataaaagga ccttttgatg
     7801 caatggtctc aggcacctgt ccgacatacg aacatgaaaa aaaggaaggc actccaaagt
     7861 ccggtatcag aacagaattc gataaagaat gtaaagttct taaaattatg aatgataatg
     7921 gaatgatttt tgaattagac cttctgcctg tgatcaaaaa gatgaatgaa aaaaccgata
     7981 agacaaatta taacctctct gttgatcaac tttcgcataa ttatgaaaat gattcagtaa
     8041 cagcaacggt tcttatcact caggcacacg gccggttaaa atcagaaaaa gaaaattcaa
     8101 ccatcagcaa tatttctgta agtgtttatt attttttaaa gattcaagag tgagtaaaaa
     8161 gtatgtcagg atttattcta agatcggatg aacttaaaaa gaacttcaag gactatatca
     8221 ttttagactg cagagaaaat cctgcggact gtaaacacat agccggatca tattttgttg
     8281 acggagaatc agtgcttact gggaaaaaag gtcagcacgg cggaaggcac cctttcccca
     8341 atatggatga atttgtaagc gacatggaaa atctcggcat ttcagatagt tcaaaagttg
     8401 cgtgctttgg agatttcgga gcaagagctg cttttatgtt gcggcttttt ggaatcactg
     8461 cttctttcat cagcggaaat tcagaaactt taaaaagatc agggattcct tttgataacg
     8521 atgatcctgc tcctgttaaa aaagggaaaa ttacaggaaa aatcaataat gatcttcttg
     8581 tgagcatgga gaaggtgcgc aaaaacatca ataatactga acacatactc attgattcga
     8641 gatccggcga aagatatcgc ggagaaaatg aaccgcttga tcccattgcc ggaagaatcc
     8701 ccaacgcagt gaacttcttc tggcaggaac ttataaaacc ggacggaacc ttcctttcag
     8761 atgaagagat cagaaaacat tttaatatcg acaccggcaa aaacatcaca ctttactgcg
     8821 gaagcggcgt aacagcttgt tttaactggc ttgcactcag acagatcgga gtcaaatgtt
     8881 caatatatac aggatcctgg agcgactgga taagttatcc ggaaaatctg gatagaatcg
     8941 aacctaaaat tcattagtgt ttcctgccac catacccctc tttactaagg gttttcatca
     9001 ggttcttgtt cacataatat ggatttctga aaaacattgt tgctccgttt gggcctgtgc
     9061 tgttgtcttt aaataatgga ggttcgtcgc ctcctccaaa attattattt aacagctata
     9121 tcaacaaaaa atatccataa aaacaaaaga attaaattta taataaacaa caatattatt
     9181 tccctccata tgcagatttc agatcgccat agcttctaga aatataatca aaaacaattg
     9241 aagcaacaaa acttacttcc ataaaaccca aaaaaaacaa gtataataaa ttacttttaa
     9301 tatttctcat tcccatgaat caacctccgg ctgaattatt cctctcattt gccacatttc
     9361 ataagtattc gaccacaaag caccgtcttt ctgagtaatc atcggcttgc cttaattgaa
     9421 ttaatctcac cttcaagcat tgaaagtccg ttaatgatgt tctcaaaaga cggatactcg
     9481 ccgaatatca tattctgcat gtctctgtaa tcatttttca tgaatttcag gacatggtca
     9541 ggcggaatga gcttcattgt tccgggaacc gccagatcat acctcgccca gcctcttgga
     9601 tagaaccttt ttttaaaagc cgttacactt tcaaggagct caatgtcttt caatgcggac
     9661 tctctgataa cacttctgct caacatgaac atatcataat aatgtcttga atatctcgca
     9721 tgcttcctga aatatgtcct ttctttcgtc agcacttaat tttacgacat tttccattgt
     9781 ttaatcctta catatctttt taattatctc atagacccat gcagttgtct tttccgtatc
     9841 tttaagcacc tttctttgtt catcgcctgt cagaaaatct cttactgaat ctatcgtgtc
     9901 aggcgaaaca ttctcttcac cgagtgcttt cagtgcctga aacactattg agctctttct
     9961 gtgtctgaac cctgaatttt tcagagcagt tcttttaaag ataaggtctg tattccctat
    10021 tttgtatttt tttgaaggtc ctgttgaaag atagacaaat ttcccctgaa cctgatttga
    10081 caatccgaga atattcaatg ccgtgtcacc tgtgggctgt atctcccatt taaactttcc
    10141 cgcaattgcc tgagcaacct ggtcaatgtc agggcttaaa aaaatgttga gcagtttatt
    10201 aaatttagga taacagtaaa cacctcttag tgcccttgcg atcattccgt caccgtttaa
    10261 ccggtgcaga gttacatcaa tggcctcagg ggaagcaata tcaagaaaat cacttctggt
    10321 aaaacaccaa cccctaccct tcccgtaaat cctgctaact attttatttt tcaaagtatt
    10381 catattcaat tccagacagt ttctgtaagg aataatattc atttttactt gcaaaagcaa
    10441 gttttataac attatcacaa tgttgatttc tacaaaaaag ggaacaacct aaaatcccct
    10501 gaaaaattcc ggtctgtgaa aatcgggctt ttcggtttta atttcaaata gatacatata
    10561 atgaggggtc ggtgaaaggt cggcacattt atagatgttt gctgaaacat ttataagatc
    10621 aggattctca tcaaaaataa gtacttcttt tggaatgaaa actttcacct gataacttgt
    10681 gacactttct tttaccccaa acggcttatt cccgagagtt gatgatactt taatcaattc
    10741 aagtgcttga ggattaataa atttccggtt gtgtctattt tcaccatatt gagcgagaat
    10801 cgaaccgatg cagtttattt caaagttgta ataattttta ttatcaaaag aaagaaaaag
    10861 ctcaacacag ctatcctgcc agaccggctg attaaaaccg gtattcacag ccctgaccgt
    10921 tggttctgtc acattgaaaa ccgctgaaat tgaatcagtt gatatattaa gatcgcactc
    10981 tactttcaga ttttcaattg gttttttaac ccagccctgt ttaagaaaat actttgccaa
    11041 aaatcattcc aaagaaagtt attgcgggat aattatacag attttcttga aaaaattaaa
    11101 gattatttag ggcagtcgcc tttcttttct ccaccctctt ttccttcttt gttcattttc
    11161 ttctgttctt ttttctgctc tttcttttcc tgtttcattt cctgtttcat ttcttttttc
    11221 atttccttgt tttcttcttt tgcttctttg tgctcttgtt ttttctcctg ctttttctcc
    11281 tgcttttttt cctgtttcat ctcttttttc tgctctttct gctctttgtg ctcctggatc
    11341 tgctctttct tcatctcttt ttcaggtgcg ggctcatcct gtgcaaaaag attaaaacct
    11401 gaaaaaatga acatcaaagc aattaaagaa atcattaatt ttgcaacttt catggtaacc
    11461 tccatcaaat tatttaaaga accgaactgt ctgtctgtca gacatccata tgacggctaa
    11521 gaatctaaaa atgttccgta tttttcagaa tttgaacgaa aattccaaca ttactctgtg
    11581 acggtcataa ttgtagtcat aataatctga gtcgctttcg cctaaggtgc tgaaattttt
    11641 gagatattta tagccaaaca ctatggcgag aaaatctgtc gcatttataa caattttaaa
    11701 agacggagct atcgcatggt ctgtcctttt tttctttatg ccgctccatt catcactttc
    11761 aaagaaatac annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naaagtaatc
    11881 gagataaagc tgctgtaccg gataaatgaa aagatctgtg gaaagatcaa gtgaaaaatc
    11941 tccaacatct ttaagcaggt cttttcttcc gccacgggtc gtacttgttt catcatcacc
    12001 gggatttgaa tcataagatg agccgatcga aacagacccg ccaccatctt caatagcggc
    12061 aagtgtctga aaacataaga ttattaacag aataaccagg gttttaagaa tgctacctgc
    12121 ccgatttttt ccgtttttcg tttcgttctt tattcttttc aagctttttt tctcttttct
    12181 cttttttcat ctctttttca gattcctttt tcgcttcctt tttctttttc agtatttcat
    12241 cttcccattt cggcgcatta tcaccttttt caacttcgac tccggttttc actttaggtt
    12301 tttttggctc ctcctttttt tcttcaccgg ttaatggagt cattgcgaaa aaaaagatga
    12361 agatcagagc aaataattct ttaagcattt atttctcctg tcgcttttac aacgatctcg
    12421 tggtttaacg atccgccgtc atattcagca gtcgcatcaa tatgccaaag ccctgcctca
    12481 acagctttta ccacaaaagg tatttcattt ctcccttttt tcagtgctcc gctccagttt
    12541 attgttttgg tgttcaatat ctcgggattt tccgaattga aggcaagccc ttttggaaga
    12601 tttattgaaa aatcgacatc atctacatca ttttcagaat cgtaaacaag ctttattgtc
    12661 acagtttctc ctgtcaaaac ctcagcggat gcaatgacag agtgctcaaa atattcttct
    12721 attccattat tttcattaac tattgttcct gcaaaaaaac ctgacgcgaa tataatgatc
    12781 accgcggcga ctgcaaaacc gcttttcata aaaagggagc cggttctttt ccgcgtcgaa
    12841 actgcactga atttagaagc catttctaaa gcaaaatttt caggaagttt tttctttttg
    12901 taacttaact gctcaaaaag ggcatctgct attttttccg gactctgttt ttccattttt
    12961 tcgtcctcca tcgttcataa gactcagaaa ctacgagaaa agttccaaat ttctttcaat
    13021 gagccggtgc agttcctctc ttgcccttga aagacggctt ttaatagaac cgaccggaac
    13081 accgagaaat tccgatgctt caacatagct cattcctgac atctctatca tcatgataat
    13141 ttcacgataa ttttcaggca gttgatctat cattctgagc aggttttctt tcctctgctc
    13201 aagcattaag ctgttttcaa tagagacaga ttccgtatcg ggctgatcgt ctgaaatatc
    13261 gccttcagtt cctgtaatct caccggactc ttttctaagt aacgatatga attcatttct
    13321 gattatcacc cacagccatg aagtgaaact gcttcttcct tcaaatgagg agatgtttag
    13381 aaacgctttg agcagtgcat tctggagaac atcgctcgcc agatcttcat tcccttttgt
    13441 tatcgaaagg gcaaacctga aaagaacctg ataatgcttt ttcaccagtt tttcaaatga
    13501 tccgccgtcc ccactctttg atctgtgaat gaagtatttt tcctgaattc ccatcaattc
    13561 tccgaaacaa agctctaagt tggacagagt atatattaaa tttgttccaa aataaacat
//
LOCUS       DAOOYB010000056        13488 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_4654_length_13488_cov_8.317427, whole genome shotgun sequence.
ACCESSION   DAOOYB010000056 DAOOYB010000000
VERSION     DAOOYB010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13488
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4654_length_13488_cov_8.317427"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(175..795)
                     /locus_tag="PL056_04965"
     CDS             complement(175..795)
                     /locus_tag="PL056_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HPM46398.1"
                     /translation="MKKNLFKENLWDEDFYLVERALNDDDLDAYDQLKYHDFYEKFVE
                     SSKILLKIIETNFDSDINDTILKMAFVYSVTTMESYFKKALLFISQQKPEFSQDVLKE
                     LNARKPHKSLANNLTSEEKVFQIINKRPLQNPKKISKLFSILGIETEKYAGLYELDEL
                     NEYIKLRHGITHRDEELEEGRKKLIDLVKRIRFFVGQINSEIETQK"
     gene            1336..1551
                     /locus_tag="PL056_04970"
     CDS             1336..1551
                     /locus_tag="PL056_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016874720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HPM46399.1"
                     /translation="MKFNVVLEKAEEGGFNVTVPALDGCFTQGDTEEEALRNASEAIK
                     CYLEGLEKLNQIKTGTKKKVRELMVRL"
     gene            1575..1763
                     /locus_tag="PL056_04975"
     CDS             1575..1763
                     /locus_tag="PL056_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HPM46400.1"
                     /translation="MVRALRRLGFVVDHQRGSHIFMHNLDRHISVIVPVHDELKKGTL
                     NNIIKKVGITLEQLKKLV"
     gene            1953..2237
                     /locus_tag="PL056_04980"
     CDS             1953..2237
                     /locus_tag="PL056_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001197622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HPM46401.1"
                     /translation="MIVVGKKLLFEYLRKNVNAKAALSAWVTEAERACWKTPQDIKKR
                     YSTASFLHGNLVVFNIKGNNYRLVVVVLYEEEIVRIKWIGTHSEYDRQKF"
     gene            2247..2846
                     /locus_tag="PL056_04985"
     CDS             2247..2846
                     /locus_tag="PL056_04985"
                     /inference="COORDINATES: protein motif:HMM:NF025742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family antitoxin"
                     /protein_id="HPM46402.1"
                     /translation="MKSEFLKIIKDDAQYEKALERIDELIALENRGDATDEIKILSLL
                     VSDYENSKYKLDFPDPVDAILFRMDQMGLKQKELVECIGSKSKVSEILSRKRPLTLSM
                     IKNLNEFLGISLDILLKSKEKNHPLNRKRKSFSMPAFEVVREKKASYKGQGKKKTKSV
                     TIRIQEDVIENIKERANDENIPYQTMINLIIKKFLESAK"
     gene            2963..>3522
                     /locus_tag="PL056_04990"
     CDS             2963..>3522
                     /locus_tag="PL056_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46403.1"
                     /translation="MKIYVAFDDTDTIDCGRGTGKLARWFEDVLPQNCKLSGIVRQQL
                     LKRADIPFTSHNSSLTCIIEAENDGIIPALIKAGTDHIANHFVEGSDPGLCVCAENSP
                     AIKNLIAFGKRCLFEKVTQDEAYLAANGCHLSGHGGTNDGIIGAAAGVGLTHNGMHGR
                     CNELKFNGMKLRDIPEKTTAEFLKSYG"
     assembly_gap    3523..3622
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3623..4510
                     /locus_tag="PL056_04995"
     CDS             <3623..4510
                     /locus_tag="PL056_04995"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HPM46404.1"
                     /translation="IEIPLKLKVPMLFFKKKLSILNTVLSGEDEILQGMIVIALIFTA
                     VYLIQVLSDYFRDVFLGTANQRIMKKIKKEIFSKVINMPEYLIKNEKAGDLMSRVTYD
                     AMVLSNLMDIFVELVRSMVYMLIFVPLMFFINWKIAFFTAIFFPLTFYFIKLLSKRVR
                     KSSRMVTDSTAEYTAFIEENIKDIGMIKKEGTEKTQIEQFDLLVENNYNDNVRLIIAK
                     SILKPSNEFMGIFGLSIASVFFSYLIVRHNFNAGNAVLFLYMMKTSYKPFKKVAQASG
                     ELFNSIVCAGKIFDLLDRR"
     gene            4516..5064
                     /locus_tag="PL056_05000"
     CDS             4516..5064
                     /locus_tag="PL056_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46405.1"
                     /translation="MLTSQRVIMIGAFEKHSGKTTLSEKIIRKFSEHGIYAVKITIHR
                     TDDPQEKYSITEDKEIRPDKDTGRLKLAGAKKVFWVRCNSNSAHIAVSEVMSMIPEDV
                     PVLCESNMARKYMKPGIFVMVKRDVAGPVKETAAEVEKFADLITVSTMKEGVIEYIPD
                     ICNILKLEGFEWKIDLIEKSTI"
     gene            5028..5828
                     /locus_tag="PL056_05005"
     CDS             5028..5828
                     /locus_tag="PL056_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPM46406.1"
                     /translation="MEDRFNRKINYLRISVTDRCNLRCVYCMPEEGVKLLRHEDILSF
                     DEITEVVKTAVELGIDKIRLTGGEPLVRKNIVELVSMIAQNSGVKDFGLTTNGILLDE
                     FALPLKKAGLHRLNISLDTMDKDKYREITRCGDISAVINGIKAAQNAGFTKIKINTVI
                     DSSPDEIDAVSVAEFARKNNFQIRFIRKMDLEKGTFWGVIGGDGGKCSICNRLRISSD
                     GTVTPCLFSDQGFNIRKIGIKEAFIQAIHSKPEKGVMSRTHKFYNIGG"
     gene            5830..6288
                     /gene="moaC"
                     /locus_tag="PL056_05010"
     CDS             5830..6288
                     /gene="moaC"
                     /locus_tag="PL056_05010"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007280996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HPM46407.1"
                     /translation="MVNKKLTHVDNEGKAKMVDVADKPDQLRTALAEGYITLKPETVA
                     MIRKNLMKKGDVLTVAQIAGIQAAKLTHQIIPLCHPLLITSIDVNLSLGDNGVKAVST
                     VKCIGKTGIEMEALSAVNGALLGVYDMCKAVDKTMVIQNIMLIEKRKEDL"
     gene            6288..7214
                     /locus_tag="PL056_05015"
     CDS             6288..7214
                     /locus_tag="PL056_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HPM46408.1"
                     /translation="MTGKIDSINISVAKGTEKTEIPSCIVTMKGIEGDAHAGLWHRQI
                     SVLDKSSIDNFSNEKDIELSPGAFGENLVIDGIDLKKVSVLDRFITGEVELEVTQIGK
                     KCHGDACSIFRRTGECIMPKTGLFTRVIKEGSINKGQSVELVERKLKFHIITMSDRAS
                     AGEYPDESGPMIEKMVQEHFSNLRWHLQIEKSIIPDEPELLKTMLIKDREDQVDFVIT
                     TGGTGIGPRDITPEVVVSLADMTIPGIMDHIRLVYGKDHPSALISRSVAAVMGKTIVF
                     TLPGSVKAVREYTQELQKNFEHLILMLHGIGH"
     regulatory      7296..7415
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            7406..8965
                     /locus_tag="PL056_05020"
     CDS             7406..8965
                     /locus_tag="PL056_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004071527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPM46409.1"
                     /translation="MCIFENQKLLSSLGIREINYGATTGLSNGWVKTTGKELQSISPV
                     DGKVIAAVIQATKEEYEYVAKKAHEAFLKFRMMPAPKRGAIVREIGDELRKHKEELGQ
                     LVTLEMGKIKMEGVGEVQEMIDVADFALGLSRQLYGLTMHSERPEHRMYEQWHPLGVI
                     GVITSFNFPVAVWSWNALVAAVCGDSIIWKPSSKTPLTAIAVQNIIAPVVDKYDLDGV
                     FSMVIGKGSDVGDAIVNDSRIPLVSMTGSTKMGIQIAQSLSKRLGRSILELGGNNAVI
                     IAEDADIDMALRAVLFGAIGTAGQRCTSTRRVIIHKSVKEKFVHALINAYKQIKIGNP
                     LDESVNMGPVADGSAIKEMLASIIALKAEGGKVLYGGETVKVPGLEGGFYITPAIAEA
                     KNEMKIVKDETFAPLLYIIEYEDFEDAIFMNNDVPQGLSSTLFTNSVRYSEMWLSHIG
                     SDCGIANVNIGTSGAEIGGAFGGEKETGWGRESGSDSWKQYMRRQTNTINWGRELPLA
                     QGIKFDLTNRK"
     gene            8977..10632
                     /gene="pruA"
                     /locus_tag="PL056_05025"
     CDS             8977..10632
                     /gene="pruA"
                     /locus_tag="PL056_05025"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013886566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HPM46410.1"
                     /translation="MTTNAIFTLPRPQNETVLDYAPGSIEREILKNELVKQKGIQVDI
                     PLIIGGKEIRTGKTVNVVSPHDHRHILGVCHLGGEKEVNMAIEAAMKVKNEWEEMDWH
                     HRASIFLKAGELLSTKYRAVLNAATMLGQSKTCYQAEIDSACELIDFYRFNAHYLQEI
                     YRTQQVHNSKGVWNRIEYRALEGFVFAVTPFNFTAIAGNLPMSPAIMGNVVLWKPAST
                     SLLSNYYIMQILKEAGLPDGVINFLPGSGGNIGDPAIDSELFSGIHFTGSTGVFRSIW
                     QRIASNIRKYRSYPKTVGETGGKDFIFVHSSANFEEVAVAAVRGAFEYQGQKCSAASR
                     MYVPRSMWKKLKPLLTEMTESIKMGDVCDFTNFMGAVIDHKSFDNTMSYITKSKESAE
                     AEVIAGGTGDDKNGYFIRPTIVETSNPHFITMEEEIFAPVLSVFVYEESKFEETMELC
                     DTTSPYALTGAIFATDRNAIIKSEKILRHCAGNFYINDKPTGAVVGQQPFGGARGSGT
                     NDKAGSHLNLVRWVSPRTIKETFVPPVSYDYKYMSDNNLCKKE"
     gene            10756..11217
                     /locus_tag="PL056_05030"
     CDS             10756..11217
                     /locus_tag="PL056_05030"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HPM46411.1"
                     /translation="MSVNVERIREVFADYAPEKGNFMVLLHEVQKTCGNWLPEEALKE
                     VAKLAEMPLNKVYGFVSFYSMFSTKPRGKHIVRVCKDGPCAVKGSIMLAELLMKELDV
                     DVDETTEDGKFTLEQTACIGTCTAAPAMMIDDEVYGNVTPESLNTILKKYR"
     gene            11228..12484
                     /gene="nuoF"
                     /locus_tag="PL056_05035"
     CDS             11228..12484
                     /gene="nuoF"
                     /locus_tag="PL056_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HPM46412.1"
                     /translation="MKFNTKILSTDNVLLKNIGSIDPLKIDDYIAAGGYEALKKAVKM
                     DRKEIIDVVSDSGLRGRGGANFPTGRKWLFTYNTQADQKYLVINADEGEPGTIKDRVL
                     MEGDPHQIIEGMVIAAYAIGATKGFIYIRGEYSDSIKTMNAAIKQAEEKGMLGKNILN
                     SGFDFEMSVAMGGGAYVCGEETALIDSMEGKRGEPRLKPPYPPVEGLFGKPTIVNNVE
                     TIVNVPHIIAKGGEWFRSLGASCSPGTKLFMLSGDVNEPGIVETPVDLSFEEVINKFG
                     KGVTGGKVKFAQIGGSSGNIFTPEMLKKVWTYERCCEFKVSYGTGSIFVCNEERGIVD
                     HLLTVAEFFEHESCGKCTPCREGTHRVREIMEKFAYGEGTVSDLELLNELCHVMKRSP
                     ICGLGQACVNPVLDAIEHYKNEILAN"
     gene            12507..>13488
                     /locus_tag="PL056_05040"
     CDS             12507..>13488
                     /locus_tag="PL056_05040"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HPM46413.1"
                     /translation="MSDKVKVVIDGIEIEVPADFTVLQAAQIAGIDIPTLCYHPDLRT
                     TGNCRVCIVEIEGMPLFQPSCAFKVYSNMKVRTNTPELRKARKMIVELIVSRHNTDCL
                     KCDSNLNCELQNLTDKMNIRDVRFEIQPDFEHPMDNSNIVQRDPSKCVNCGRCIRMCN
                     EIQTVNALEWTGRGYHKMAMTTMEKPLAETACISCGQCINVCPVGALTERDDTEIAWS
                     FIDDPEIHTVVQTAPAIRAGLGEACGLEQGHLSAGKMFAAIKKVGFDKAMDTDFTADL
                     TILEEGTEFLGRVTRAVKDGATDVAFPMFTSCSPGWINFMETFYPELLPNL"
BASE COUNT         4357 a         2353 c         3024 g         3654 t
ORIGIN      
        1 actttcggag gtttttgtca agttttttat tgaaaatcga acgatttagg taataattta
       61 atgtgaaaaa actttaaaaa tgaaagattt aaccaaaagt tttatgttga tagcggagat
      121 ttagaagatt caataataga aaatgttgga gaaattggta tccattccag cggactattt
      181 ctgggtttct atttcactgt ttatctgacc cacaaaaaac cttattcgtt ttacaagatc
      241 gattagcttt tttctacctt cctcaagttc ctcatcccga tgagtaatgc cgtgtcttaa
      301 tttaatatac tcatttaatt catccaattc atatagtcca gcatattttt ctgtttctat
      361 acccaaaata gaaaaaagtt ttgagatttt cttagggttc tgaagtggac gcttattaat
      421 tatctgaaac accttttctt ctgaggttaa attgttggca agagacttat ggggcttacg
      481 agcattcagt tcctttaaaa catcttgtga aaattcaggc ttttgttgtg aaataaaaag
      541 tagcgctttt ttaaaataac tttccatagt tgtgaccgaa taaacaaaag ccatcttcaa
      601 tattgtgtca ttgatgtctg aatcaaaatt tgtctcaatg attttcagaa gtattttaga
      661 tgattccaca aatttctcat aaaaatcatg atattttaat tgatcgtaag catcaagatc
      721 atcatcgtta agtgcccttt caacaagata gaaatcttca tcccacaaat tttctttaaa
      781 taaattcttc ttcataaact cctccttctt gtttttgtat taaaactcaa cataccatga
      841 ttacttagca taaactcaaa attccaagca attttcttta aatctttcac catcttcacc
      901 tccaataatt caaaaaaaca gacaccgttt ctcaaaactg agatctcata gtttttgaga
      961 aaaatgatcg atcatctcat atataatacg gaaaaaaata aaatctgaat aaaataggtg
     1021 ttccggtttg aaacacaagt caaaataatt caatgttttc aatgtgttgc agaattgtct
     1081 atttgctgct taacaaaaac agaggacata tctcaataaa tatgtaatag gagggagcaa
     1141 tatttatata taaaactgta acttgaataa ccccaactct ctaaataaat tccacttttt
     1201 ctctgttttc tggcgagcaa gatattctta tttgttttct tgctcttcca ctcacttcct
     1261 cgcacgcttt gatttgacaa tggtgctgtc cacgagtaaa ctatttaccg ctaacatttt
     1321 gaagtgaggc gtgatatgaa atttaatgtt gttcttgaaa aagccgaaga aggcggattc
     1381 aatgttactg ttccggcgct tgacggttgt tttacgcagg gagataccga agaagaagct
     1441 ttgcgtaatg catccgaagc aattaagtgc tatctcgaag ggctcgaaaa actcaatcag
     1501 atcaaaaccg gaacaaagaa aaaagtaaga gaactaatgg tcagattatg agcaaaacat
     1561 tttcagggcg ggaagtggtt agagctttaa ggcggctcgg ttttgtggtt gatcatcagc
     1621 gtggcagcca catttttatg cacaatcttg accgccatat ctctgtcatt gttccagttc
     1681 acgatgaact aaaaaaggga acactcaaca acatcatcaa aaaggtcgga ataactctcg
     1741 aacaacttaa aaaactggtc tgattccaaa accagaataa acgacaacac cctaaataaa
     1801 ctccactttt cccagttttt aggcgagcaa gataatctta ttcattttct tgcatattta
     1861 attcatttgt tgcattatct atcggttgat taaattaatg ttcgcaagtt gggaactttt
     1921 gtgttgacaa atatttgtga agaattaaga taatgattgt tgttggtaaa aaactgctct
     1981 ttgaatattt gagaaaaaat gtaaatgcaa aagcagctct ttcagcatgg gtaactgaag
     2041 ctgagcgtgc atgttggaaa acaccgcagg acataaaaaa gagatacagc acagcaagct
     2101 ttttacatgg caatttagtt gtgttcaaca taaaaggaaa caattaccgc cttgtggtcg
     2161 ttgttcttta tgaggaagag atcgtgcgaa taaaatggat tggaacacat tcagaatatg
     2221 acaggcagaa attttagggg gaaatggtga aaagtgaatt tttgaaaata ataaaagatg
     2281 atgctcaata tgaaaaagca cttgaaagga ttgacgaatt gatagctctg gaaaatcgtg
     2341 gtgatgcaac ggatgaaatc aaaatcctgt ctttgctcgt gtcagactat gaaaacagta
     2401 agtataaatt ggatttccct gatccggttg atgcgattct tttcaggatg gaccagatgg
     2461 gcttaaaaca gaaagaactt gttgagtgta tcggttcaaa aagcaaggtt tcagaaatat
     2521 taagcagaaa aagaccttta actctgagca tgataaaaaa cctgaatgaa tttcttggaa
     2581 tatctctgga tattcttctt aaaagtaagg agaaaaatca tccgttaaat agaaaaagga
     2641 aaagtttttc aatgcccgct tttgaagttg tacgggaaaa gaaagcgagc tacaaaggtc
     2701 agggaaagaa aaaaacaaaa tctgtaacca tcaggattca ggaagatgta attgaaaaca
     2761 ttaaagaaag agcaaacgat gagaatattc cttaccagac gatgataaac ctcataatta
     2821 agaaattttt agaatccgca aagtagccgg tttttcaaaa atggatctca aaatgacaaa
     2881 aaaactggtc agcatatttc ttgcattttc aattgtttaa attacgattt ccgggcgtta
     2941 tgaatttgtt tttgaggtaa aaatgaagat atatgtcgct tttgatgata ctgatactat
     3001 tgactgcggt agagggacgg ggaaacttgc aagatggttt gaagatgttc ttcctcaaaa
     3061 ctgtaaactt tcagggatag taaggcagca gcttttaaag cgggctgaca ttccgttcac
     3121 ttcgcataac agttcgctta cctgcatcat tgaagcggaa aatgatggaa taattccggc
     3181 acttataaaa gcgggaaccg atcacatcgc taaccatttt gttgaaggga gcgatcctgg
     3241 actttgtgtc tgtgcggaaa acagccccgc tataaagaac ttaattgctt tcgggaaaag
     3301 gtgtctattt gaaaaagtta ctcaggatga ggcatatctt gctgcaaatg gctgtcatct
     3361 ttcgggacac ggcggaacaa atgacggaat aatcggagcg gcggcaggtg tcggactgac
     3421 ccacaacggg atgcacggaa gatgcaacga actaaagttt aacggaatga aactccgtga
     3481 tattcctgaa aaaactactg cagaatttct taaaagttac ggnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnattgaaat tccgcttaaa cttaaagttc cgatgctttt
     3661 tttcaaaaag aaactttcca ttttaaacac tgttcttagc ggcgaagatg aaattcttca
     3721 gggaatgatc gttattgctc tcatttttac cgccgtttat ctgattcagg ttctaagtga
     3781 ctatttccgc gatgtttttc ttggcactgc caatcagcgg atcatgaaaa aaataaagaa
     3841 agagatattt tcaaaagtca ttaacatgcc ggaatactta ataaaaaatg aaaaggccgg
     3901 agacctcatg tcaagagtga catatgacgc catggtcctt tcaaatctta tggatatttt
     3961 tgttgaactt gtcagaagca tggtctatat gcttattttt gttccgctga tgttctttat
     4021 aaactggaag atcgcttttt tcactgcgat cttttttccg cttacatttt attttataaa
     4081 acttttatca aaacgggtca ggaagagttc aagaatggtt acagacagca cagcggaata
     4141 tactgcattt attgaagaaa atataaaaga tatcggtatg atcaaaaaag aagggacaga
     4201 aaagactcag atagagcagt tcgatctgct tgtcgaaaac aattataatg acaatgtccg
     4261 cctgataatt gcaaaaagca ttcttaaacc gagcaatgaa ttcatgggta tattcggtct
     4321 ttcgatcgct tctgttttct tttcgtatct gatagtcagg cataatttta atgcaggcaa
     4381 tgcagtttta tttctctata tgatgaaaac gagttataaa ccgtttaaga aagttgcaca
     4441 ggcatcggga gaacttttca attccatcgt ctgtgcagga aagatatttg atctgcttga
     4501 caggaggtga taagtatgct gacttctcaa agagttatca tgatcggagc ttttgaaaaa
     4561 cattcaggca agacaacgct ttctgaaaaa ataataagaa agttctctga acatggaatc
     4621 tatgcggtaa agataaccat acaccgaact gacgatccgc aggaaaagta ttccataact
     4681 gaagataagg agatcagacc cgataaagac acagggcggc ttaaacttgc aggggcgaaa
     4741 aaggttttct gggtgagatg caattcaaat tcagcacata ttgctgtttc cgaagtgatg
     4801 tccatgattc ctgaagatgt gccggtactt tgtgaatcaa acatggcccg caagtatatg
     4861 aagccgggga tttttgtcat ggtgaaaaga gatgtggcag gtccggttaa ggaaacggcg
     4921 gcagaagttg aaaaatttgc cgatctgatt actgtttcaa ccatgaaaga aggagtgatt
     4981 gaatacattc ctgacatttg taatattcta aaactggaag gatttgaatg gaagatagat
     5041 ttaatagaaa aatcaactat ttaaggatat cagtcaccga caggtgcaat ttgagatgtg
     5101 tatattgcat gccggaagaa ggggtgaagc ttttaagaca cgaggacatt ctctcttttg
     5161 atgaaataac tgaagtagtg aaaacggcgg ttgaacttgg aatagataaa atacggctta
     5221 ccgggggaga accgcttgtc aggaaaaaca ttgttgagct tgtttcaatg atagctcaaa
     5281 atagcggtgt gaaggatttc gggcttacaa caaacggaat actgctggat gaatttgccc
     5341 tgccgcttaa aaaagccgga cttcacagat tgaacatcag tcttgacacg atggataaag
     5401 acaaataccg tgaaattaca agatgcggag atatttcagc agtaattaac gggataaaag
     5461 ccgctcagaa tgcagggttc acaaagataa aaattaacac ggtaattgat tccagtccgg
     5521 atgagatcga tgcggtatct gttgctgaat ttgcccgtaa aaacaatttc cagataagat
     5581 ttatcagaaa aatggacctt gaaaaaggga ctttctgggg agtgatcggc ggtgacggcg
     5641 gaaaatgttc gatatgtaac cggctgagaa tttcttctga tggaactgta acaccgtgtc
     5701 ttttcagtga tcagggtttt aatatcagaa agatcgggat aaaagaggcg tttattcagg
     5761 cgatacactc caagccggaa aaaggggtga tgagcagaac acataaattt tataatatcg
     5821 gagggtagtt tggtaaacaa aaaactgaca catgttgata atgaaggaaa ggcgaagatg
     5881 gttgatgttg cagacaagcc cgatcaattg agaactgcac tggctgaagg ttatataact
     5941 ttaaaaccgg aaacagttgc gatgatcagg aaaaatctca tgaaaaaagg ggatgttctc
     6001 actgttgcgc agattgccgg aattcaggcg gcgaaattga cacatcagat aattccgcta
     6061 tgtcatccgc tccttataac ctcaattgat gtcaatcttt cgttgggtga taacggtgtg
     6121 aaagctgttt caactgtaaa gtgcatcgga aagacaggga tagaaatgga ggcgttaagt
     6181 gctgtgaacg gagcgctgct gggcgtttat gatatgtgca aagcagttga taaaacgatg
     6241 gtcattcaaa acattatgct cattgaaaaa agaaaggagg atctgtgatg acaggaaaga
     6301 tcgattcaat aaacatatcg gttgcaaaag ggactgaaaa aacagagata ccttcatgca
     6361 ttgtgaccat gaaagggatt gaaggagacg cgcacgcggg tctgtggcac aggcagatca
     6421 gtgttttaga taaaagctcg atagataatt tctcaaatga aaaggatatt gaactttctc
     6481 ccggtgcatt tggggaaaat cttgttattg acggtatcga tctgaaaaaa gtatctgttc
     6541 ttgacagatt tataactggt gaagttgaac ttgaagtgac ccagatagga aagaagtgtc
     6601 atggcgatgc gtgttccata ttcagaagaa cgggtgagtg cataatgcct aagacaggac
     6661 tgtttacaag ggttataaaa gaaggatcca ttaataaagg ccaaagtgtt gaacttgtgg
     6721 aaagaaaact taaatttcac ataataacca tgtctgacag agcttctgca ggcgaatatc
     6781 ccgatgagag cggtccaatg atcgaaaaaa tggtacagga acatttttca aacctcagat
     6841 ggcatctgca aattgaaaag agcataattc cagatgagcc ggaacttcta aagacgatgc
     6901 tcattaaaga tagagaggat caggttgact ttgttattac gaccggaggc acaggtatcg
     6961 gacccagaga tataactcct gaagttgttg tttcccttgc ggacatgacg atccccggca
     7021 ttatggacca tatccggctc gtgtacggca aagatcatcc atctgcactg atttcccgct
     7081 ctgttgcggc agtaatggga aaaactatcg tgttcactct tcccggaagc gtaaaagctg
     7141 tcagagaata cacacaggaa cttcagaaaa attttgagca tctcatatta atgcttcacg
     7201 gaatagggca ctgacccgtt caggtgtttc cacggtctta tttttattta ctttctaaag
     7261 aagatttgtt aaaagtaagt gtctttagtt ctgttaaata ttaaaaactc tgaagtcttc
     7321 tgtcctaaag cgtctgccat ggacttaaga ccaacgggtc ggctctgaaa agatccggtc
     7381 tgccgttatt gcaaaggaga aatttatgtg catttttgaa aatcagaaac tgttatcttc
     7441 tcttggtatc agggaaatta attacggagc aacaaccggt ttatcaaacg gatgggtgaa
     7501 aacgacagga aaagagctcc agagtatctc accggttgac ggtaaagtca ttgcagcagt
     7561 tattcaggcg acaaaagaag agtatgaata tgttgcgaaa aaagctcacg aggcattttt
     7621 aaaattcaga atgatgcctg ctccaaaaag aggcgccatt gtccgggaga tcggggacga
     7681 attaagaaaa cacaaagaag agctgggtca gcttgtcact ctcgaaatgg gcaagatcaa
     7741 gatggaagga gtgggcgaag tgcaggaaat gatagatgtt gccgattttg ctctgggact
     7801 cagccgccag ctttacggat tgactatgca cagcgaaaga ccggaacaca gaatgtatga
     7861 acagtggcat ccgctcggag tgatcggtgt tattacatca ttcaattttc cagtcgcagt
     7921 ctggtcgtgg aatgcgcttg ttgccgcggt ctgcggagac agcataatat ggaaaccgag
     7981 ttcaaaaacg ccgcttacag ctattgcagt tcagaacatc attgctccgg tcgttgacaa
     8041 atacgatctt gacggagttt tctcgatggt gataggaaaa gggagcgatg tgggtgatgc
     8101 aattgtgaac gatagcagaa ttcctcttgt aagcatgaca ggaagcacaa aaatgggtat
     8161 tcagatcgct cagtctcttt caaaaagact tggcagaagc attcttgaac ttggtgggaa
     8221 caacgctgtt attatcgctg aagatgcgga cattgacatg gctctcagag ctgtactttt
     8281 cggtgcaata ggtactgccg gtcagagatg tacatcaaca agaagggtca taattcataa
     8341 gtcggtaaaa gagaaattcg tccatgctct gataaatgcc tataaacaga taaagatcgg
     8401 caacccgctt gatgaaagcg tcaatatggg tcctgtggct gacggttctg ctataaaaga
     8461 gatgcttgct tcgattattg ctctcaaagc tgagggaggc aaagttcttt acggtggtga
     8521 aacagtgaaa gtgccggggc ttgaaggcgg cttctatatc actcctgcaa tagctgaagc
     8581 gaaaaacgaa atgaagattg tgaaagatga aacttttgca cctcttcttt acataataga
     8641 gtatgaggat ttcgaagatg cgatattcat gaacaacgat gttccgcagg ggctgagttc
     8701 aacacttttt acaaacagtg tcagatattc tgaaatgtgg ctttcccata tcggttctga
     8761 ttgcggaata gctaatgtca acatcggcac aagcggagct gagatcggag gagctttcgg
     8821 tggtgaaaaa gagaccggct ggggcaggga atcaggctca gattcatgga aacagtacat
     8881 gagaagacag accaatacaa ttaactgggg gagagagctg cctctggcac agggaataaa
     8941 atttgatctt acaaacagga aataaaggag aaacaaatga caacaaatgc aattttcacc
     9001 cttccgcgtc cgcagaatga aactgttctg gattatgctc cgggatcaat agaaagagaa
     9061 atattaaaaa atgagcttgt aaaacagaaa ggtattcagg ttgacatccc tttgataatc
     9121 gggggcaaag agatcagaac gggaaaaacg gtcaatgtcg tctctccaca cgatcacaga
     9181 catattctcg gagtatgtca cctcggtggt gaaaaagagg tcaacatggc aattgaagct
     9241 gccatgaaag taaaaaatga atgggaggag atggattggc atcaccgtgc atctatattc
     9301 cttaaggcag gagaacttct ttcaacaaaa tacagagcgg ttcttaatgc ggccacaatg
     9361 cttggacaga gcaagacatg ctatcaggcg gaaattgact cagcttgcga gctgattgat
     9421 ttttaccgtt ttaatgcaca ctatttgcag gaaatataca gaactcagca ggtacacaac
     9481 tcaaaagggg tgtggaacag aattgaatac agggcgcttg aagggtttgt ctttgctgta
     9541 actcctttta attttactgc aattgcagga aatctgccta tgtctcctgc tatcatggga
     9601 aatgttgtct tatggaaacc tgcttcgact tcacttttat caaactacta catcatgcag
     9661 atacttaagg aagccggact tccggacgga gtaataaatt ttctccccgg aagcggcggt
     9721 aatatcggtg atcctgcaat agattctgaa ctgttttcag gaattcattt taccggctca
     9781 actggggttt tcagatcaat ctggcagaga atagcaagta atatccgcaa atatagatca
     9841 tacccgaaaa ctgtcggtga aacaggcggc aaggatttca tttttgttca cagcagtgca
     9901 aattttgagg aagttgctgt tgccgctgtc agaggggctt ttgaatatca gggacagaag
     9961 tgttctgccg cttcaagaat gtatgttcca agatcgatgt ggaagaaact taaaccgctt
    10021 cttactgaaa tgacagaaag cataaaaatg ggtgatgtgt gtgatttcac caattttatg
    10081 ggcgctgtaa tagaccataa aagttttgac aatacaatga gttacataac aaaatcgaaa
    10141 gaaagcgcag aagctgaagt aattgcaggc ggaacaggtg atgataagaa tggatatttt
    10201 atccgtccga caattgttga aacctcaaat cctcatttta taacaatgga agaagagatt
    10261 ttcgcaccgg tgctgtctgt ttttgtgtat gaagaaagta aatttgagga aactatggag
    10321 ctttgtgaca caacttctcc ttatgccctt actggagcga tttttgcgac tgacaggaat
    10381 gctataatta aatctgaaaa aatattgagg cattgtgccg gcaactttta tatcaatgat
    10441 aagccgactg gtgctgttgt aggtcagcag ccgttcggag gagcaagggg aagcgggaca
    10501 aatgacaaag ccggcagtca cctgaatctt gtacgatggg tcagcccgag aacaataaaa
    10561 gagacattcg ttccaccggt gagctatgat tacaaatata tgagtgataa caatctgtgt
    10621 aaaaaagaat aaaatgttga aaaaaaatga aaaaaatgtt gaaagaaaag aaaattttgt
    10681 atatacaaat gcaactggtt gttttttctt gagattaagc gatattaaat tgttattaga
    10741 agttaggagg agtttatgag tgtaaatgtt gaaagaattc gcgaagtttt tgcggattat
    10801 gctcccgaga agggtaattt tatggtttta ctgcatgaag tgcagaaaac atgcggaaac
    10861 tggcttcctg aggaggcatt aaaggaagtt gcaaaacttg cagaaatgcc gctgaacaag
    10921 gtttacggct ttgtctcttt ttattctatg ttctcaacaa aacccagagg aaagcacatt
    10981 gtcagggttt gcaaggacgg tccctgtgca gtaaaaggtt ccataatgct tgctgaactt
    11041 ctgatgaaag agcttgatgt tgatgttgat gaaacaaccg aagacggaaa gttcacactt
    11101 gagcagaccg catgtatcgg aacatgcact gccgctccgg caatgatgat cgatgatgag
    11161 gtttacggga atgttactcc tgaatctctg aacacaatac tgaaaaagta cagataagga
    11221 ggataatatg aaatttaaca caaaaatcct ttcaaccgat aatgttcttt tgaaaaacat
    11281 cggttcaata gatcctttga aaatcgacga ttacattgca gccggcggat atgaagcact
    11341 taaaaaagcc gtgaaaatgg acagaaagga gatcattgat gttgtaagtg attccggtct
    11401 tcgcggtaga ggcggagcaa atttcccgac agggagaaaa tggctgttta cctataacac
    11461 acaggcagat cagaaatatc ttgtgatcaa tgctgatgaa ggtgaaccgg gaacgatcaa
    11521 ggacagagtt cttatggaag gcgaccccca tcagatcatt gaagggatgg tcattgccgc
    11581 ttacgctatc ggtgcgacga agggatttat ctatatcaga ggcgaataca gcgattcaat
    11641 aaagacaatg aatgccgcaa taaaacaggc ggaagaaaaa gggatgctcg ggaagaacat
    11701 tcttaacagc ggattcgatt ttgagatgag cgttgcaatg ggcggcggtg cctatgtatg
    11761 cggtgaagag accgcgttaa ttgattcaat ggaagggaaa agaggtgagc cgcggcttaa
    11821 acccccttat ccgcctgttg aaggtctttt cgggaaaccg acaatagtca acaatgtaga
    11881 aacaattgtc aatgttccgc acattattgc aaagggaggc gaatggttca gatctcttgg
    11941 cgcttcatgc agtcccggaa caaaactttt catgctttcc ggtgatgtta acgagcccgg
    12001 catagttgaa acacctgttg atctttcttt tgaagaagtg atcaataaat tcggaaaagg
    12061 tgtaaccggt ggaaaagtga agttcgccca gatcggtgga agttcaggaa acatattcac
    12121 tccggaaatg cttaaaaaag tatggactta tgaaaggtgc tgcgaattca aggtaagtta
    12181 cggaaccggc tcgatttttg tatgtaacga agaaagaggc attgttgatc atcttcttac
    12241 tgtagctgaa tttttcgagc acgaatcatg cggaaaatgc actccatgca gagaagggac
    12301 acacagagtc agagagatta tggaaaaatt cgcttacggt gaaggaacag tcagtgatct
    12361 ggaacttctc aatgaactct gtcatgttat gaagagaagt cctatctgtg gtcttggaca
    12421 ggcatgtgtc aaccctgtgc ttgacgcaat tgagcattat aagaatgaaa ttttagcaaa
    12481 ctgattaaaa acagaaggag tgaaaaatgt cagataaagt aaaagttgtc attgacggga
    12541 tagaaatcga agttcccgct gattttacag tgcttcaggc agcacagatc gcaggaatag
    12601 atattccaac gctttgctat catcctgatc tcagaacaac cggaaactgc agagtttgta
    12661 ttgtcgagat cgaagggatg ccccttttcc agccgtcatg tgcatttaaa gtttacagca
    12721 atatgaaagt aaggacaaac acaccggaac ttagaaaagc acgcaagatg atcgttgaac
    12781 ttattgttag ccgtcacaac acagactgtc tcaagtgtga cagcaacctc aattgcgaac
    12841 tgcagaatct tacagacaaa atgaatatcc gtgatgtccg ctttgaaatt cagcccgatt
    12901 ttgagcaccc gatggataat tcaaatatcg tacagagaga tccttccaaa tgcgtaaatt
    12961 gcggaagatg catcaggatg tgcaacgaga tccagacggt caacgctctt gaatggacag
    13021 gtcgcggata ccacaaaatg gcaatgacaa caatggaaaa accgcttgca gaaactgcat
    13081 gtatttcctg tggtcagtgc ataaatgtat gtcctgtcgg tgctctcact gaaagagatg
    13141 acacagaaat tgcgtggtcg ttcattgacg atcctgaaat tcacacagtt gttcagactg
    13201 ctcctgctat cagagcgggt cttggtgaag cttgcggact tgaacagggt cacctgagtg
    13261 caggaaaaat gttcgcagca ataaagaaag tgggattcga caaagcgatg gataccgatt
    13321 tcacagccga tcttacaatt cttgaagaag ggacagaatt cttaggcaga gttacaagag
    13381 cagtaaaaga cggtgcgacc gatgttgctt ttccgatgtt cacatcatgt tcaccgggct
    13441 ggatcaattt catggaaact ttctatcccg aacttcttcc gaaccttt
//
LOCUS       DAOOYB010000057        13452 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_4676_length_13452_cov_12.142121, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000057 DAOOYB010000000
VERSION     DAOOYB010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13452
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4676_length_13452_cov_12.142121"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="PL056_05045"
     CDS             <1..462
                     /locus_tag="PL056_05045"
                     /inference="COORDINATES: protein motif:HMM:NF014171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HPM46414.1"
                     /translation="TDFPKVYLDSPLSDAMRRMIKKGVIELPVLEKNGKLAGCIHEHD
                     IINFYNREILSKGSLLKTVNKIEGTEQSFIQFEDEYRIEVFNSTPSMWGKNLIDLRFR
                     EKFGILVLAVKEKKTGKNILSPTKVLEPGDVLVAAGTKDDLTLFKEFDKKS"
     gene            540..2195
                     /locus_tag="PL056_05050"
     CDS             540..2195
                     /locus_tag="PL056_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPM46415.1"
                     /translation="MLKENLVGSFERTLKDNWEKPCFSDYGMSPVTFCHVAVKIKEFH
                     GLFEMLGIGKGDRIAVAGKNSTNWAIIYLATVSYGAVIVPVLSDFAVDEIHHIVNHSG
                     SKLFFSSESIYDRINDESMTGLKVIFSLENFAVLRDYSNKKEKIDEFIEEFRSKNELS
                     PEKFSFDEISSEKLASIVYTSGTTGFSKGVMLSHNCLMANVQFFIDNLPVENDSRVVS
                     FLPLAHCFGCAFDFLSPFVKGCHITFLGKIPSPKIILKAFEEIKPVIIFAVPLILEKI
                     YRAKIMPVLESAKMKILMKIPGLKNLIIKKIGRSINEAFGGNHYEIIVGGAAFNPEIE
                     EFFMKAGVRVTVGYGMTECGPLISYRDYYKGRPVGSCGKVINYLDIKIDSPDEHGVGE
                     ICVKGENIMDGYYKMDEQTAEAIDSEGWLHTGDLAYIDSEGFIFISGRSKNMILSSSG
                     QNIYPEEIEAKINFMAYVAESLVLDSGNGKLVAYVYPDKEKMSANNITEEMVAEIMKE
                     NRVKLNEVLPLFAKVTDIRLYPEEFEKTSTKKIKRRLYTSLVK"
     gene            2283..3260
                     /locus_tag="PL056_05055"
     CDS             2283..3260
                     /locus_tag="PL056_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HPM46416.1"
                     /translation="MEFSQLNERIREQAIAVNTLRAEISKVIVGQQDMVDSLIMGMIG
                     EGHILLEGVPGLAKTTAVKAFADATDLEFKRIQFTPDLLPADIIGSRIFNINDRNFEI
                     KKGVIFSNVILADEINRSPAKVQSALLEAMQERQVTIGDTTFKLPDPFIVLATQNPLE
                     QEGTYPLPEAQVDRFMFKVLIDYPSKDEELKIVERFEKNELLQKLNRVIGNGEIKKWQ
                     ETVKAIYTDERIKKYVISIVFETREPKDKSVSQLIDVGASPRASIFLILAGRIRAFME
                     GRNFVTPEDIKEVAYPVLRHRIIPSFEAEAEKISTDRIIGELLNRVEVP"
     gene            3266..4129
                     /locus_tag="PL056_05060"
     CDS             3266..4129
                     /locus_tag="PL056_05060"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HPM46417.1"
                     /translation="MLNEELFKKIRYIELNVKKKVDNYLQGSYHTLFKGQGLEFQEVR
                     EYTENDDARLIDWNVTARTGKPFVKVFREERELTVMIAADISGSTLYGKSSGLREVMA
                     TIAAAISFSAIKNSDKVGLLLFSDRIEEYIPPRRGRNHLLTVIRALLTATPKGRKTDP
                     EVPFKFLNKVMKRKTLTFFLSDMMFDKLPESLNITASKHEIVPVGVIDETIFTPLVQG
                     AVKVLDPETGETSILDLSIPGLNDMTKFIAAHEAGFKRKGIRPVWIMNRENFMDELVN
                     EFKKLSSVRKK"
     gene            4126..4986
                     /locus_tag="PL056_05065"
     CDS             4126..4986
                     /locus_tag="PL056_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46418.1"
                     /translation="MRKAALLILLLFSQIVFCEDLKTEVSKESVYLGEVFTLKAGTLA
                     KENMTAVFAGETKDVAILGVEKGDDGNVIIKMITLVSGELTVPQIDLTIDGNVFNIGQ
                     FKVSSMNRTAENDMNLRDIKDTVKIMEKDYTLLYVLAFLLAIAALIFLFLKLRKRFAK
                     KESSITQKVEPSEVAAKFIKEAKAKRESGDNEAFVDLSTLGLKTYMSLISDLNYTEMT
                     TSEVKRALKKDKLFSAATDVIVGILKLGDRFKFADDTLSESDFDRIIDGFAGTVRETE
                     KGRVVKNDAA"
     gene            4973..5959
                     /locus_tag="PL056_05070"
     CDS             4973..5959
                     /locus_tag="PL056_05070"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPM46419.1"
                     /translation="MMLLDPLWLLLIIPALIITAFQLRIKGSGSSIFYNNFKAGRKKA
                     SGIYFLPNIIDLLAVIMAVLALARPVSLEKTVTPPVEGKDIMVVLDVSGSMEALDFQP
                     KNRIEAAKSVIESFVKGRTSDRLGLVFFAKDSFLQVPLTTDYDIFIELLSMLKTGVIE
                     DGTAIGNGLGLAVSRLENSSAKSRIVILLTDGDNNAGNVSPANAAQYASAKGVKIYSI
                     LIGTDAEVPFPAGKDFFGRDTFQKVQMKTNPDLLKQISNVSGGKFYQSISTDELKKAF
                     RDIDELEKSPVPARKLKIYNEYAPLFIMLAVIFILLARLIAMKFRIYPEVER"
     gene            5956..6927
                     /locus_tag="PL056_05075"
     CDS             5956..6927
                     /locus_tag="PL056_05075"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPM46420.1"
                     /translation="MKFYDWTYFLTAVIWIPVIFLWTVYSSKKRNDFLKLFSKSQTEK
                     IVDISQRKIVVKRVLIFLALSFFTVSAARPLMGGEEIKADSSGIDIAVVFDVSLSMYA
                     EDESGPRFEKGRKLLIDVLSSLTGDRVAIIPFAGAAFLQLPLTDDYETLQTVVSVLEP
                     GMIERQGTALGNALEMAVETLTSSGNESDRMIVVISDGEDPELNFEKVRKLIKENGIH
                     LAVVPMGTVEGAPVRIGESYLKDENDNTVISKLDRGFFEKCFETLGAVEIKKGTTISE
                     HVKNFKKAIRNENRNLNIYTERFQMPLFAGIIIFWLFQLLSLGRRRS"
     gene            6924..7661
                     /locus_tag="PL056_05080"
     CDS             6924..7661
                     /locus_tag="PL056_05080"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPM46421.1"
                     /translation="MKNILLISTVFIFLAGFTPFEKETPESREGRKKYAAGDMEGAKS
                     EFEKASERLENSAEAYYDLGNSLQRSGDNEGALNSYRKSLETGGADNQLKAKIFHNMG
                     NSLAAMQKYKEAEDFFIRSLMEDPQDDTAENLEIVRRIIRQQEQQKQEQQKQDQEQKE
                     EQKQDQEKEDQQQEQKEDQQQEQKEEQKEDQQQEQNAAQAEEKEEEEKKEDGKEEMMQ
                     QFRQRKNLQISPFMLKKDARSKNGQTW"
     gene            7674..9410
                     /locus_tag="PL056_05085"
     CDS             7674..9410
                     /locus_tag="PL056_05085"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HPM46422.1"
                     /translation="MNKFSIVHKFLILFALLAISNSISAQSVTLSTDSSVVSAGEVFR
                     FILTAEGSFDSIIEPDLSNFDVDNRSESQSSSISIINGKMESSKSINYTYFLRAKKAG
                     VFKIGPASVKLKNGRKKDSNIVTVTVTGSSAGSSDQTEGEDSKSGENPEPTANSLLAP
                     LTTWEKRTPNHFIRAVVSPAGEVYEGEPVVVKYYIFTKPNAISDLNFYKTPSFENCWK
                     EEKNESRLSFKRGAIDGNVYDYGLLKTYYLIPEKGLAEISGTQMIVDVMTGSFFSTRK
                     QSVSTPALRIPLTPIPEKESHNEGFFADISVTADKNSVTLDKDNLLETVTFTINGCGN
                     LQSAVAELLPTPELKYFSPEVELNAGGTADGYCGQKKYRFMVKGLKKGKFSIKAKDIE
                     TFSREKGWQVLKMPEITVDVKEVSNSTDSVSESVSQRFELLRELPEGIKKYENTPLIK
                     RNWFMVMLAVPFLMVMISFLIWLSGSMRENRSKSRRARIEKWKEKLNIANSLNELLNI
                     FYDALKEIYSIELRGERKHNVKKKYGDSTGDILAFIKDIEFASYSSNADSDISAFKSK
                     AIGFLDIRGVKK"
     gene            9407..10117
                     /locus_tag="PL056_05090"
     CDS             9407..10117
                     /locus_tag="PL056_05090"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HPM46423.1"
                     /translation="MRKTAVFIFLTVSFLSLFANEDGFSLYLKKNYKEAYNAFNEQFI
                     SSNGDPLYAYNLGVTSTALGQKGSAVYFYIQALQRAPELPEAKNNLKILAGEMNITIP
                     QVLFEPVHSIDYLLMIFIVSIYVFAIFVSILCFKNDWRIKTALLPVFLIMTVSAAMYF
                     MNYEEENKINWAVAVSGDSLRSGPDSSLTEIGKIREGEIINIVSSSGSWYKVKSFQDN
                     VEGWIELKSIRAVMRGHI"
     gene            10114..10833
                     /gene="bamD"
                     /locus_tag="PL056_05095"
     CDS             10114..10833
                     /gene="bamD"
                     /locus_tag="PL056_05095"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HPM46424.1"
                     /translation="MNYRILLVFSVALTVVFSYSCKSAPVIKGAESDGEVVYKQGLYY
                     LEKGDYVNAENKFMKVISDFSYSIYEPFATVALGDTYFYKEEYPSAVEVYSRFVKMRP
                     THEKTPWAELQMANSYLKQMPSDFFIFPSPSERDVDIVEKAVVQFRHYLSKYPDDGNR
                     EKGIKDLEKAELVLIERDLRVAEYYAKKKRCASANTRIKYISDNFTVTTEKNKKRIGA
                     ILKKCPEVKEEKKPEEKTDAK"
     gene            10823..11632
                     /locus_tag="PL056_05100"
     CDS             10823..11632
                     /locus_tag="PL056_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46425.1"
                     /translation="MQNRCSAIILFFSLIAGIFSSCNLSQDQKANIVKILEIRESLVN
                     SGDVKSLEVLMTEDFPDKKEYLDQMTYRYQYFLKFSYDMNTIDILSSSVLGNRAHLML
                     SYDLSFRNPDDVAETFWLDRKEDVIMVKEKIGWKIADIKEMKNTGRKIDPQTVHDIFF
                     ALDTRKTALNNGDNELFSTIIWEKYPDREKLVDNFKKNASAFINVNYGLKSRRFQYVS
                     PSMDEARVIQYFDLVFNIKGLDEAEKIEDQSELISLKKMPDNTWKITDGLK"
     gene            11813..12418
                     /locus_tag="PL056_05105"
     CDS             11813..12418
                     /locus_tag="PL056_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6088 family protein"
                     /protein_id="HPM46426.1"
                     /translation="MQGIDNKVIKRIRGWGSGSVFTPTMFLNLGSRAAIDKILSRFSQ
                     KGIIRRLSRGLYDLPKKHPVMGKLYPSPEAVAKAISDRDSTKILPSGAYATNLLGLST
                     QVPAQIIFLTDGASRSVKVGGHTITLRRTTPKNMATAGRISGLVIQALKYIGKDHVDP
                     SIIQMLTETLNETDKEQLLKDLVFAPSWIGKIIRKIAEEVK"
     gene            12415..13422
                     /locus_tag="PL056_05110"
     CDS             12415..13422
                     /locus_tag="PL056_05110"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HPM46427.1"
                     /translation="MINFILLSSEERKLYFEQVASSMKVSPLIIEKDFWVCYTLHKLF
                     SLPLIGETLIFKGGTTLSKIYDIIQRFSEDIDISIDRKSLNSEGHDPAETGIGTKEKQ
                     RRLSKLADLCRVKINEEIQPLLSEAIGKDVPNRDWVLEKDDSDPDGQTLSFKYPSVLI
                     NMKSGYIRPAVKIEMGARSDHWPSEIHSVISYVAQQYPQPFKNPSTKVKVLSIQRTFF
                     EKATLLHAEYHRPVEKTMPSRLSRHYYDMAMLMQKGVKIDEGLLEKVAEHKKIFFQSG
                     WAKYDEAKKGTLHVLPNSQRLKALEEDYKRMEEMFFVKPITFSEILAILSSWEKEFNE
                     F"
BASE COUNT         4414 a         2402 c         3027 g         3609 t
ORIGIN      
        1 actgatttcc ccaaggtata tcttgattcc cctttgtccg atgcaatgag aaggatgata
       61 aaaaaggggg tgatagaact tcctgtcctt gaaaagaatg gcaaacttgc cggatgcatt
      121 cacgagcacg acatcattaa tttttataac agagagatcc tttcaaaagg gagtctgctc
      181 aaaaccgtta ataaaattga aggtacagag cagagcttca ttcagtttga agatgaatac
      241 agaatagagg tttttaattc gaccccatca atgtggggta aaaatctcat tgatctccgt
      301 tttcgggaaa agttcggaat acttgttctc gcagtaaagg aaaaaaagac ggggaaaaac
      361 atactttctc cgacaaaagt tcttgaacct ggagatgttc tggtagctgc aggaacaaaa
      421 gatgacctca ctcttttcaa agagtttgat aaaaaatcat aaaatcaggt ttttttgttg
      481 attttgcttg ataaaaaagt agactcaagt gtcagttcta tgctttttgg agaggagcga
      541 tgcttaaaga gaatcttgta ggttcatttg aaagaacgct gaaagataac tgggaaaaac
      601 catgtttcag tgattacgga atgagtcctg tcacattttg tcatgttgca gtaaagatca
      661 aagaattcca tgggttattt gaaatgctcg gaatcggaaa aggtgaccgt atcgccgttg
      721 cagggaaaaa cagcacaaac tgggcgataa tttatcttgc cacagttagc tatggtgctg
      781 taattgttcc ggtattaagc gattttgctg tggatgagat acatcacatt gtcaaccatt
      841 cgggatcaaa attgtttttt tcgtcggaat cgatatacga cagaataaat gatgagtcaa
      901 tgactggact caaagtaata ttctctcttg aaaattttgc agtactgcgt gattattcaa
      961 acaaaaaaga aaaaatagac gaatttattg aagaattccg cagtaagaac gaactttcac
     1021 ccgaaaaatt ctcatttgac gagatcagtt ctgaaaagct tgcttcaatt gtttacactt
     1081 caggaacgac cggattttca aagggcgtca tgctttcaca caactgtctg atggcgaatg
     1141 tccagttttt tatagataat cttcctgttg aaaatgattc aagagtcgtt tcatttcttc
     1201 ctctcgcaca ctgtttcgga tgtgcttttg attttctaag ccctttcgtt aaagggtgtc
     1261 acataacatt ccttggaaaa attccgtcac ccaagatcat tctcaaggca tttgaagaga
     1321 taaaaccggt gataattttt gcagttccgc tcattcttga gaaaatttac agagcaaaga
     1381 taatgcctgt tcttgaatcg gcaaaaatga aaatattgat gaaaatacct ggacttaaaa
     1441 atcttataat aaaaaagatc ggccgcagta taaacgaggc attcggcgga aatcactatg
     1501 aaattattgt gggtggagcc gctttcaatc ctgagataga ggaatttttc atgaaagcgg
     1561 gtgtcagagt cactgtcgga tacggaatga cagaatgcgg gccgctgatc agctatcgtg
     1621 attattacaa gggaaggcct gtaggcagtt gcggaaaggt gataaattac cttgatatta
     1681 aaattgacag ccctgatgaa cacggcgtag gagagatctg cgtaaaaggt gagaacatca
     1741 tggacggtta ctacaaaatg gatgaacaga ccgctgaagc cattgacagt gaagggtggc
     1801 tccataccgg agatcttgca tatattgaca gtgaaggatt cattttcata tcgggcaggt
     1861 caaagaacat gatactttcc tcatcaggtc agaacattta tcctgaagag atcgaagcaa
     1921 agataaattt catggcttat gtggctgaat cgcttgttct tgattccgga aacggaaagc
     1981 ttgtcgccta tgtctatccg gataaggaaa agatgtcagc gaacaacatt actgaagaaa
     2041 tggttgcgga aataatgaaa gagaacagag ttaagctcaa tgaagttctt cctctatttg
     2101 caaaagtgac cgatatcagg ttataccccg aggaatttga aaagacatcc actaaaaaga
     2161 tcaaaagacg gctttacaca tctttagtga aatagatcca ctcttttttg attttttctg
     2221 aaataaaact actatcaccg gtgtttataa gtcagttttt ttgtaatgat ggaggaatgt
     2281 tcatggaatt cagtcagctt aatgaaagga tcagggagca ggcgatcgca gttaatactt
     2341 tgagagcaga gatctcgaag gtgatcgtgg gtcagcagga catggtcgat tctcttatca
     2401 tggggatgat aggtgaaggg catattctcc ttgaaggtgt tcccgggctt gcaaaaacta
     2461 cggcagtaaa agcatttgca gatgccacag accttgaatt taaaaggatc cagttcactc
     2521 ccgatcttct tccggcagac atcatcggaa gcagaatatt taatattaat gaccgtaatt
     2581 tcgaaataaa aaagggagtt atcttttcaa atgtgatcct tgccgatgag ataaacagat
     2641 ctcccgcaaa agttcagtcg gcactgcttg aagcgatgca ggaaagacag gtgacaatag
     2701 gtgacacgac tttcaaactt cccgaccctt tcattgttct tgcaacacag aatcctcttg
     2761 aacaggaggg gacatatcct cttcctgaag cacaggtgga cagattcatg tttaaagtgc
     2821 tcattgatta tccttcaaaa gacgaagagc ttaagatcgt tgaaagattt gaaaaaaatg
     2881 aactccttca gaaactgaac agagttatcg ggaacggtga aataaagaag tggcaggaaa
     2941 cagttaaagc gatatatacc gatgaaagaa taaaaaaata tgtaatttca atagtttttg
     3001 aaacaaggga acccaaggac aaatccgttt cccaactcat tgatgtcgga gcaagcccga
     3061 gagcgagcat ctttcttatc cttgccggaa gaattagagc tttcatggag ggaagaaatt
     3121 ttgtaactcc tgaagatatc aaggaagttg catatccggt gcttcgacac agaattattc
     3181 cgtcgttcga agcggaagcg gaaaaaatat cgacagacag gataatcggt gaacttttga
     3241 acagggttga ggttccttaa tatccatgct caatgaagaa ctttttaaaa agataagata
     3301 tatcgagctc aatgtaaaaa agaaagtcga taactacctt caggggtcat accacaccct
     3361 tttcaaaggg caggggcttg aattccagga agttcgcgaa tacaccgaaa atgacgatgc
     3421 ccggctcatc gactggaatg ttacggcaag aacaggaaag ccgttcgtca aagttttcag
     3481 agaggaaaga gagcttacag tcatgatcgc agcagacatc tccggctcga ctctttatgg
     3541 aaaaagttca ggattaagag aggttatggc aacaattgcc gctgcgatat ctttcagtgc
     3601 gataaaaaat tctgacaagg tcggactcct tcttttttca gaccgcatcg aagaatatat
     3661 tcctccaaga agggggagaa accatcttct gactgttatc agagcactgc tgaccgccac
     3721 tcccaaagga agaaagacag acccggaggt cccttttaaa tttctgaata aagttatgaa
     3781 aaggaagacg ctcacatttt ttctttcaga catgatgttt gataagttgc cggaatcttt
     3841 gaatataact gcttcaaaac acgaaattgt ccctgtcgga gtgatcgatg agacaatatt
     3901 tactcctctt gtccagggtg ctgtaaaggt ccttgaccct gaaacgggtg agacatccat
     3961 tcttgatctg agcattccgg gactcaacga catgacgaaa ttcattgcgg cacatgaagc
     4021 tggctttaaa agaaaaggga taagaccagt ctggattatg aaccgtgaga attttatgga
     4081 tgaacttgtc aatgaattca agaagttaag cagcgtgaga aaaaaatgag aaaagcggcg
     4141 ttgttaatat tgctcctttt ttcacagata gttttctgtg aagatctaaa gaccgaagtg
     4201 tcaaaagaga gtgtatattt aggggaagtt ttcaccctca aagctggtac tttagcaaaa
     4261 gagaacatga ctgcagtttt tgcaggtgag acaaaagatg tcgctatcct tggggttgag
     4321 aaaggtgatg acggcaatgt cataattaaa atgataactc ttgtcagcgg tgaactgaca
     4381 gttccgcaaa tagatctcac tatagacggg aatgtgttta atattgggca gttcaaagtt
     4441 tcgtcaatga accgtactgc tgaaaatgac atgaacctgc gggacataaa agatactgtt
     4501 aaaataatgg aaaaggatta tacacttctt tatgttcttg cttttcttct tgcgattgcc
     4561 gcacttatat tcctgttctt aaagctcaga aaaagatttg caaaaaaaga gagctcaatc
     4621 actcaaaaag ttgagccgtc tgaagttgcg gcaaagttca tcaaagaggc aaaggcaaaa
     4681 agggaatcgg gtgataatga agcgttcgtt gatctttcaa cgctggggct taaaacatac
     4741 atgtcattga taagtgactt aaattatacg gaaatgacca catcggaagt gaaaagggct
     4801 ttaaagaagg acaaattgtt ttccgccgct acagatgtaa ttgtcggaat acttaagctt
     4861 ggtgacagat ttaagttcgc tgatgataca ctgtctgaaa gtgactttga caggatcatt
     4921 gacggatttg cgggaaccgt aagagaaact gaaaaaggaa gggttgtaaa aaatgatgct
     4981 gcttgatccg ctatggcttc tgctgattat tccggcatta attatcacag cgttccagtt
     5041 gaggataaag gggtccgggt catcgatatt ctataataat tttaaagccg gcagaaaaaa
     5101 agcatccgga atttattttc tccctaatat tattgatctt ctggctgtaa ttatggcagt
     5161 tctcgctctt gcaagacctg tttctctcga aaagacggtg actccgccgg ttgaaggaaa
     5221 ggatataatg gtcgtacttg atgtgagcgg ttcgatggag gcgcttgatt tccagccgaa
     5281 aaacagaata gaagcggcaa aaagtgtgat cgaaagcttc gtcaaagggc ggacgagcga
     5341 ccgtctcggg cttgtatttt ttgctaaaga cagctttctg caggttcccc tcacaacaga
     5401 ttacgatatt ttcattgagc ttctttcaat gctcaagaca ggtgtcattg aggatggaac
     5461 tgcgatcggc aatggtctcg ggcttgctgt gagccggctt gagaacagct ctgcaaaaag
     5521 caggatagtc attcttctaa cagacggcga caacaatgca ggaaatgtca gtccggcgaa
     5581 tgcggctcag tatgcttcag ccaaaggggt taaaatatat tcaattctta tcgggacaga
     5641 tgctgaagtg ccgttccccg caggaaagga tttcttcggc agagatactt tccagaaagt
     5701 tcagatgaag accaaccctg atcttttaaa gcagatttcg aatgtttcag gcggaaagtt
     5761 ctatcagtcc atttcaacag atgaactgaa aaaggcattc agggatatcg atgaacttga
     5821 aaaaagtccc gttcccgcaa gaaagctgaa aatatataat gaatacgcac cattattcat
     5881 tatgttggcg gtaatattca tactccttgc cagacttatt gcgatgaaat tcaggatata
     5941 tccggaggtg gagagatgaa attctacgac tggacatatt tcctgacagc ggttatatgg
     6001 atacccgtga tattcttatg gacagtttat tcttcaaaaa agaggaatga ttttcttaaa
     6061 cttttctcaa aaagccagac tgaaaaaata gttgatattt cccaaagaaa aattgttgta
     6121 aaaagggttc tcatatttct cgctctctca tttttcacag tttccgcggc aagacctctc
     6181 atgggtggcg aagagataaa agccgacagc tcaggaatag atatcgcagt agtatttgat
     6241 gtttcattaa gcatgtatgc cgaagatgag tcaggaccca gatttgaaaa ggggagaaag
     6301 cttctgatcg atgtgctgag ttcactcaca ggtgacagag tcgcaataat tccttttgcc
     6361 ggagccgctt ttcttcaact tcctctgaca gatgactatg aaacgctcca gacagtggtt
     6421 tccgtccttg agccgggaat gatcgaaagg cagggaacag ctctgggaaa tgcactggag
     6481 atggcggttg agacgcttac atcttcaggg aatgaatctg acagaatgat agttgtaata
     6541 tctgacggag aggacccgga acttaatttc gaaaaggtga ggaagctcat aaaggagaac
     6601 ggaattcatc ttgcggtcgt ccctatgggt acagtcgaag gagcaccggt caggatcggt
     6661 gaaagttacc tgaaagatga gaacgataac actgtaatat caaaacttga cagaggtttt
     6721 tttgagaaat gttttgaaac gctcggtgca gtcgagataa aaaaggggac aacgatcagt
     6781 gaacatgtta aaaactttaa aaaagccata agaaacgaga atagaaacct caacatctat
     6841 actgaacggt tccagatgcc tctttttgca ggaattatca tattctggct tttccagctt
     6901 ctctctttag gaaggagaag atcatgaaaa acatattact gatctctaca gtttttattt
     6961 ttcttgccgg ttttaccccg tttgaaaaag aaacccccga aagccgcgag gggagaaaaa
     7021 aatatgccgc aggtgatatg gaaggggcaa aaagtgaatt tgaaaaagct tcagaacggc
     7081 ttgagaacag tgcggaagct tattatgatc tcggaaattc gttacagaga tcaggagata
     7141 atgaaggtgc tttgaattca tacaggaaat cgcttgaaac aggcggagct gacaatcagc
     7201 taaaggcaaa gatatttcac aacatgggta attcgcttgc ggcaatgcag aaatataaag
     7261 aagcagaaga ttttttcatc agatcattga tggaagatcc gcaagacgat actgctgaaa
     7321 atcttgaaat agtaagaaga ataattcgtc agcaggaaca gcagaaacag gaacagcaga
     7381 aacaggatca ggaacagaaa gaagaacaga aacaggatca ggaaaaagaa gatcaacagc
     7441 aggaacagaa agaagatcag cagcaggaac agaaggaaga acagaaagaa gatcagcagc
     7501 aggaacagaa tgctgcacag gcggaagaga aagaggaaga ggaaaaaaag gaagacggaa
     7561 aagaagaaat gatgcagcag ttcagacaga ggaaaaattt gcaaatatct cctttcatgt
     7621 taaagaaaga tgcaaggtca aaaaatggtc agacctggta gtggcggatt tatatgaaca
     7681 aattcagtat cgtacataag tttctgattt tatttgcatt gcttgcgata tcaaattcaa
     7741 tttcagctca gagtgtaacc ttgtcgaccg acagcagtgt cgtttcagca ggcgaagttt
     7801 tcagattcat tcttactgcg gaagggtcat tcgattcaat aatagagccg gatctgagca
     7861 attttgatgt agataacagg tcggaatcac agtcgagctc aataagcatc atcaatggaa
     7921 aaatggaatc gagcaaaagc attaactata catattttct cagagcaaaa aaagcaggtg
     7981 ttttcaagat cgggccggca tctgtaaaat tgaagaacgg aaggaagaaa gattcaaaca
     8041 ttgttacagt gacagtaaca gggtcttctg ccggaagcag tgatcagacg gaaggtgaag
     8101 actcaaaatc cggtgaaaac cccgaaccga ctgccaattc tctgcttgcg ccacttacaa
     8161 catgggaaaa aaggactccc aatcatttta tcagagctgt tgtgagtcct gcgggagaag
     8221 tttatgaagg tgaaccggtc gttgtgaagt actatatttt cacaaaaccg aatgccatat
     8281 cagatctcaa tttttataag acaccttctt ttgagaattg ctggaaagag gaaaagaacg
     8341 agagccgcct gagtttcaaa aggggtgcga tagacgggaa tgtttatgat tacggattgt
     8401 taaagacata ttatctgata cctgaaaaag ggttggccga aatttcaggg acacaaatga
     8461 tagttgatgt gatgaccggc tcgttcttta gcacaagaaa gcagagcgtt tccactccgg
     8521 cattgaggat tccgctgact ccgatccctg aaaaagaatc gcataacgaa gggttctttg
     8581 cagatatttc ggtaactgcc gacaagaatt ctgtcacact tgacaaagat aatcttctcg
     8641 aaacggtaac tttcactatc aacggttgcg gaaacctgca gtcagctgtg gccgaacttc
     8701 tgccgactcc tgaacttaaa tacttttctc ctgaagtgga acttaatgcc ggtggaactg
     8761 ctgatggtta ctgcggacag aagaagtaca ggttcatggt gaaaggattg aaaaaaggga
     8821 agttcagtat aaaagcgaaa gatattgaaa cattcagcag agaaaaagga tggcaggtct
     8881 tgaaaatgcc cgagatcacc gttgatgtta aagaagtttc aaattcaacg gattcagttt
     8941 ccgaaagtgt gagtcagaga tttgaacttt taagggaact gcctgaggga ataaaaaaat
     9001 atgaaaacac cccgctgata aaaagaaact ggtttatggt aatgcttgcc gttccttttc
     9061 tgatggtaat gatatctttc cttatctggc tttccggtag tatgagagaa aaccggtcaa
     9121 aaagccgcag ggcgagaatt gaaaagtgga aagagaaact taatattgca aattcactta
     9181 acgaacttct caatattttt tacgatgccc tcaaagagat atattcgata gagctcaggg
     9241 gtgaacggaa gcataatgtc aaaaagaaat atggtgattc gacaggcgac atacttgcat
     9301 tcattaaaga tattgaattt gcatcctatt cgagcaatgc agattccgat atatcagctt
     9361 tcaagtcaaa ggcgatcggg tttcttgata tcagaggtgt aaaaaaatga gaaaaactgc
     9421 tgttttcatt tttttaactg tttcatttct atctcttttt gcaaacgagg acgggttctc
     9481 tctctatctc aaaaagaatt ataaggaggc gtacaacgct ttcaacgaac agttcatcag
     9541 ttccaacggc gacccgcttt atgcctacaa cctcggtgtg acctctaccg ctctgggaca
     9601 gaagggcagt gctgtatatt tttatattca ggcgcttcag cgggcaccgg agcttcccga
     9661 agcaaagaac aacctgaaga tccttgccgg tgagatgaat atcactattc cgcaggtact
     9721 ttttgaaccg gttcattcaa ttgattatct tctcatgata tttattgttt cgatatatgt
     9781 ttttgcaatt tttgtttcga ttctctgttt taaaaatgac tggagaataa agacggcgct
     9841 tctccctgtt tttcttataa tgaccgtttc tgcggccatg tatttcatga attacgagga
     9901 agagaacaag atcaactggg ccgttgcggt gagcggggat tctctcagaa gcggtcctga
     9961 ctcatctctg actgagatag gaaaaataag agaaggggaa ataataaata tagtatcttc
    10021 gagcggttcg tggtataaag tgaagagttt ccaggacaat gtagagggct ggatagagtt
    10081 gaaaagtatt cgggcggtta tgagaggtca tatatgaact ataggatttt attggttttt
    10141 tctgttgcat tgacagttgt tttttcatat tcctgcaaat cggctccggt cattaaagga
    10201 gcggaatcgg atggagaagt agtatacaag cagggtctgt actacctcga aaaaggggat
    10261 tatgtcaatg ccgagaacaa gttcatgaag gtgatctcag atttcagtta cagtatttat
    10321 gaaccttttg ccacagttgc gctgggtgac acatattttt ataaagagga atatccttcc
    10381 gcagttgaag tttacagccg ttttgtgaaa atgagaccga ctcatgaaaa gactccgtgg
    10441 gctgaacttc agatggcaaa cagttatctt aagcagatgc cgtcagattt tttcattttc
    10501 cccagtcctt cggaaagaga tgtcgatatt gttgaaaaag cagttgttca attcaggcac
    10561 tatctttcaa agtatcccga tgacggaaac agggagaaag ggataaaaga tcttgagaaa
    10621 gcggaactgg tacttatcga gagggatctt cgggttgctg aatattatgc taaaaagaag
    10681 agatgcgcct cggcaaatac aagaattaaa tatatttcag acaatttcac agtcactact
    10741 gaaaaaaaca aaaagaggat cggagcgatc ctgaaaaaat gtccggaagt caaagaagaa
    10801 aagaagccgg aagaaaaaac agatgcaaaa tagatgttcc gctatcattc tgttcttttc
    10861 tctaatcgcg ggaatatttt catcctgtaa cctttctcaa gatcagaaag ccaatatagt
    10921 gaagatcctt gaaattagag agtctcttgt caactcaggc gatgtcaaat ctcttgaagt
    10981 tctgatgaca gaagatttcc ctgataaaaa ggaatatctt gaccagatga catacagata
    11041 tcagtatttt ctaaaatttt cttatgatat gaacacgata gacattcttt cgtcatcggt
    11101 cctgggcaac agagcccacc tgatgctgag ctatgatctt tcattcagaa acccagatga
    11161 tgtggctgaa actttctggc ttgacaggaa agaagatgtc atcatggtaa aagagaagat
    11221 cggctggaag atagctgata taaaggaaat gaagaacaca gggagaaaga tagatccaca
    11281 gactgttcac gatatatttt ttgcacttga tacaaggaag accgctctga ataatggtga
    11341 taatgaactc ttttcaacga taatctggga aaaatatccc gacagggaaa agcttgttga
    11401 taatttcaaa aagaacgcct ctgcgttcat caatgtcaat tacggtctca agagcagaag
    11461 attccagtat gtttcaccct caatggatga agcaagagtc atccagtatt ttgatcttgt
    11521 tttcaacatc aaagggcttg atgaagccga aaagatcgaa gaccagtcag aactcatctc
    11581 attaaagaaa atgcccgaca acacatggaa gatcactgac gggttgaagt agtatttttt
    11641 caccacctaa agtaaaattt ccaaataatg atgaaataag ttatacttcc gacgcattaa
    11701 acagacgagt gacgaaagtg gtggatgggg ttcatgactc ttcaactttt cttttcttga
    11761 cttgtcagaa aaagtatata atatttctga cagataattg attcgaggat gcatgcaagg
    11821 tattgataat aaagtgataa aacgcataag aggatggggg agtggttctg tgttcactcc
    11881 aactatgttc cttaatcttg gaagtcgtgc ggcaattgat aaaattcttt caagattttc
    11941 acaaaaaggt attatcagga ggttgtccag aggtttatat gatctgccga aaaagcatcc
    12001 tgtgatggga aagctttatc catctcccga ggctgttgca aaagctatat cagacagaga
    12061 ttcaacaaag attctgccgt caggagctta tgcgacaaat cttctcggtc tttcaacaca
    12121 agtccctgct caaatcatat ttcttactga cggggcatcc cgttctgtca aagtcggagg
    12181 tcatacaata actctccgtc gcacaactcc caaaaatatg gccacagccg ggaggataag
    12241 cggactggtt atccaggcat tgaaatacat aggaaaagat catgtcgatc cttcaattat
    12301 tcaaatgttg acagaaactt taaatgaaac agacaaagaa cagttattga aggatttagt
    12361 attcgctcct tcatggatag gtaaaataat aagaaaaata gctgaagagg tgaaatgata
    12421 aattttattt tattgagttc tgaagaaaga aaactctatt ttgaacaggt agcttcttca
    12481 atgaaagtgt ctcccttgat aattgaaaaa gatttctggg tttgctacac acttcacaaa
    12541 ctattttcac tgccgcttat tggagaaacc ctaatattta agggaggaac gacactttca
    12601 aagatatatg acataattca acggttttct gaggacattg atatttcaat agacaggaaa
    12661 agtttgaata gtgaaggaca cgatcctgct gaaacaggta tcggaacgaa agaaaaacaa
    12721 agacggcttt ctaaactggc tgatttgtgc agagtaaaaa ttaatgaaga aattcagccg
    12781 cttttatctg aagcaatcgg gaaagatgtt ccgaatagag actgggtact tgaaaaagac
    12841 gattcagatc cggatggaca gacactatct tttaaatatc cgtctgtgct gatcaacatg
    12901 aaaagtggtt atatcagacc ggcagtaaaa atcgaaatgg gtgcacgatc tgatcattgg
    12961 ccaagtgaaa tacacagcgt aatttcctat gttgctcaac aatatccgca accatttaaa
    13021 aacccgtcaa cgaaagtaaa ggttttgtca atacaaagaa ctttttttga aaaagcaacg
    13081 ctgcttcatg ccgaatatca tagaccagtt gagaaaacta tgccgtcaag gttatcccgt
    13141 cattactatg atatggcgat gctcatgcaa aaaggggtga aaattgacga agggcttctt
    13201 gaaaaagttg cggaacacaa gaaaatattt tttcaaagtg gctgggcaaa atatgatgaa
    13261 gccaaaaaag gcacgctgca tgttttgcca aatagtcaaa gattaaaagc acttgaagaa
    13321 gattataaga gaatggaaga aatgtttttt gttaagccga taactttttc tgaaatactg
    13381 gccattcttt catcatggga aaaagagttt aatgaatttt gaattttcat ggcggcaacc
    13441 ttctcaaagt aa
//
LOCUS       DAOOYB010000058        13343 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_4731_length_13343_cov_10.368829, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000058 DAOOYB010000000
VERSION     DAOOYB010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13343
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4731_length_13343_cov_10.368829"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1139
                     /locus_tag="PL056_05115"
     CDS             <1..1139
                     /locus_tag="PL056_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005909290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HPM46428.1"
                     /translation="DSPKVAFDKAAEFITKKEPISIAYHGNIVDLLEYADKNNCHIDM
                     LSDQTSCHAVYDGGYCPAGITFEERTKLLGTDKHKFNKMVDETLRRHYEVIKKLVSKG
                     TYFFDYGNSFMRAVFDAGVKEISKNGVDTFEGFIWPSYVEDILGPELFDYGYGPFRWV
                     CLSGKEEDLIKTDAAAAEVIKTCREELRYQDKDNYQWVKDAMKNKLVVGTQARILYQD
                     AFGRTRIAKKFNEMVKNGEIGPIMLGRDHHDTGGTDSPYRETSNIKDGSNIMADMATN
                     IFAGNCARGMSLVALHNGGGVGIGKSINGGFGMVLDGSARVDAILDIAFPWDVMGGVS
                     RRAWARNEHSIETCIEYNEINKNNDHITLPYIVKDELIKKVCKL"
     gene            1218..2405
                     /locus_tag="PL056_05120"
     CDS             1218..2405
                     /locus_tag="PL056_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM46429.1"
                     /translation="MIKRKVFNRIFNILCESRKFIHVLAGPRQVGKTTVINQVIEEIN
                     IPSAYFSADDSTQTGKLWIQMNWEAARIKVRTEKLNDFILVFDEIQKIPDWTEMIKKL
                     WDEDSLSKMPIKVVLLGSSTLLIQQGLTESLAGRFEITRLTHWSYPEMEQAFSFSVEQ
                     YIYFGGYPGAATLINDEERWKNYVRDSIVETTISKDILLMKRIDKPALLRQLFYLGAS
                     YSGQILSLNKMMGQLQDAGNTTTLSHYTELLDKAGMVKGLQKYASQQFRQRNSSPKFI
                     VYDTAFLTVQLGISFEKAVSDRALWGRVAESAVGAYLVNQCVGKDIELFYWRESPDEV
                     DFVLKKGENIVAIEVKTGFKNESLKGMSKFIQSFPKSKPLLIGTGGIPVADFFKIEIE
                     ELF"
     gene            complement(2414..3646)
                     /locus_tag="PL056_05125"
     CDS             complement(2414..3646)
                     /locus_tag="PL056_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46430.1"
                     /translation="MRITLLMFFVLSIQTLSADYLFEKDLVVIGRNATALKTFFSVEH
                     DSMLIINELQTNSFFYAESNKSLEILNKMNKNIFWGSSIQNLLPVEGGVVITVEFAGT
                     IEKIFAKTVVLAESTKLGKALIEKQHQSQPFQRYFAECYEDFRIIEPAGNIFLANAET
                     FFVTEKNNPELLAGMMVSNLVKNKLNGKACIFPDGISIAGIPTDETMKHIKKIKPKMF
                     GLVGDKPDLDWITNEKNTEKLGYKFERFFGSWSFEFLKNLKSEDISNIDMLGYCPQFN
                     EISEELWAERIDFLKERFKNTKLLVGPCVDLYGTINQRTNSADYSGFEIKNEKIAKYL
                     NGDYDGYIENTLKNRNEIGVFITYYEKFLNSEEFILATEKIMRDGHLFGVTTNPDNYF
                     LFELIYQNSCRKVAEYKD"
     gene            3878..4354
                     /locus_tag="PL056_05130"
     CDS             3878..4354
                     /locus_tag="PL056_05130"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46431.1"
                     /translation="MWSGRAKDIFNKLEPLANSMGLKIVELDLPIGQNGVFRIYLDTM
                     SADKGITVDECGRFSPIVSDYLDTEDMFPFRYYLEVSSPGLDRPYRRWEDLHLAIGKT
                     LKIKLSETVEGRKRITGTLISVSDEKGEFTVDSEGTMITIAKGFVKKINEIWKGEK"
     gene            4354..5916
                     /gene="nusA"
                     /locus_tag="PL056_05135"
     CDS             4354..5916
                     /gene="nusA"
                     /locus_tag="PL056_05135"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HPM46432.1"
                     /translation="MDFNLKELVDQLVKMKGIKRELIVDILKEALYQAVKKKLGSDAD
                     IDIMFDDATGELQAFYFREVVEGEGDPQLDINIEEARKLDPEVQIGDTLGVKMEADEL
                     GRIAAQVAKQVIVQKIKTLEGDIIYNEFKDRQNEIITGTVRRFEKGGIIVDIGKTEAY
                     LPYSQHIPSDRFRINDRIKAFITEIVKTPQGCNILLSRINTMFLIKLFELEVPEIAEG
                     IVKIEGVARDPGHRSKISVRSIDHDVDPVGACVGMKGSRIQNVVHELSGEKIDIIPWD
                     EDSVRYICNTLSPVEVSQIIIDEDEHSMDVIVPDDQLSVAIGRGGENVRLASQLTGWS
                     IDIQSESQMAMMLEEAKRRLLFIDGIDESIADSLIKLGYTTLEDLTSVAPEVLSELPD
                     ITTEKAESIINQAKKMLEKGMGMIDIDDDLEEKLNVPTTTLKCVNEELAEYIAEKGYH
                     TLADIQSEPDTETFAKNAELSLRRARQIRYSLQIHIDDLNGKKRGKDGRVNADSFFDF
                     SRIEENGEDEKN"
     gene            5903..6502
                     /locus_tag="PL056_05140"
     CDS             5903..6502
                     /locus_tag="PL056_05140"
                     /inference="COORDINATES: protein motif:HMM:NF016204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF448 domain-containing protein"
                     /protein_id="HPM46433.1"
                     /translation="MKRTDRERTCFACNGTDSKENLMKWVAVAGRILPDWLQKIEARS
                     IYTHYSKNCISAFLNSRKLPEKFFHGIPVFEIGQERIFSFLKERSVDSISHFLSICRK
                     SGVVFKGQNLIMEKAKKGQKFKYLICASDVSENTVSDVERVTGMHCFKTGLDKSAVGA
                     MFDGRPTGVFALVESAQSEKLFFYMNLLEKFISGDKYAH"
     gene            6492..9137
                     /gene="infB"
                     /locus_tag="PL056_05145"
     CDS             6492..9137
                     /gene="infB"
                     /locus_tag="PL056_05145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HPM46434.1"
                     /translation="MRISLLSKEIGVGSKEIMEFLKSKGLDYSSHFNVLNPDEEKLVR
                     EKFSSGLPVAEAEDDSKKTVTTIRRSGAQRQRSFIPHSQRESAEEGALFKNLDEQVGT
                     DDQEHPVSAENRTSEENDNTADNKSKTDESGTNDKKSEEHVHEHEHDRRLRVEEEARR
                     LLAGDSGTEDVIEKVEKDEKDTSKKDDKKKIEVLDEIIDDDDLDLKSKHKKRKVVIPD
                     NETPVVANIPAQRHNKVRKNIKNVEPANVSLKVKGKIKGPDIAQFIEDPYAIQREARR
                     RKRDRRKMKPVAVVPANPTKAIKKVLKLEDTVQVSELARRMGVKASQLISKLMVMGEV
                     TGINDRINYEMAQLLAEEFGFTAENVALNEETYIEISEVREDHLVSRPPVVTVMGHVD
                     HGKTKLLDAIRHTNVVDGEAGGITQHIGAYSVELERGKITFLDTPGHEAFTAMRSRGA
                     QATDVVILIVAADDGVMPQTVEAINHAKAANVPMIIAINKIDKPDANPQKVKNELLKY
                     EIVAEELGGEHLFVEISAKQRINIEGLLESVLLQVEMMDLKADPKKPAEGIVIESKMD
                     PGKGPVATVLVKDGTLNIGDLVVTGTSMGFVRSMMDWTGSRVKDAGPSMAVEITGLDF
                     VPPAGEKLHVFKDEKKAKGLVDLRCNKLREKKHEKESRMSLENLFTKMELGDVQDINI
                     VVKGDVDGSVEAIISSLKKIEHKKMNVKIIHAGVGGINENDVLLASASNGIIVGFNVR
                     IDNKAKQLAVSEMVDIRIFSIIYELIDSIKSAMTGMLAPIIKEELSGKLEVRDIFKVG
                     KVGTIAGCMVVEGRIVRGGKVKLVRENVVIYEGKLGTLKRFKDDVKEVRNGYECGATI
                     DNFNDIKVGDVIECYIDKEIRDEVV"
     gene            9137..9508
                     /gene="rbfA"
                     /locus_tag="PL056_05150"
     CDS             9137..9508
                     /gene="rbfA"
                     /locus_tag="PL056_05150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HPM46435.1"
                     /translation="MAGQIRQSRIATAIKKFVSTLMLSEYGDSPVSMITISKVTVMPD
                     LRSAKIFYSTFDSGSVDKIQEYLDLKTKNIRFKLASHLKTLKFAPEIKFVYDDSVEKI
                     IRLEKIFDSIKSDQNGSGNDE"
     gene            9501..10472
                     /locus_tag="PL056_05155"
     CDS             9501..10472
                     /locus_tag="PL056_05155"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HPM46436.1"
                     /translation="MSDFRKINEIIDSSRSILIAGHEMPDGDSMGSCIAMASILERMG
                     KDVKVYRSGQFPFNYMFLKGSGNACESIPEGPFDLFVILDSGSPERVGEDLYKLMASS
                     TGKKILFDHHTLKKENDDFFDAVYSDTSACATAAIVYRWAADRGMALNKEESEAVYSA
                     IISDTGGLKYGSTNKESFIILSELLDRVNPWDVATRIYENVPVEQLKMLSEVLADLRI
                     LAEGKAAVIRITLDQFERYKLHPDHVDSFVNYARSIKGVQIAMRFREKGPGTWKVSMR
                     SRGNIDANEVASRFGGGGHKNAAGFVFKGTFEEGLKAVEEIVRSVSN"
     gene            10459..11343
                     /gene="truB"
                     /locus_tag="PL056_05160"
     CDS             10459..11343
                     /gene="truB"
                     /locus_tag="PL056_05160"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HPM46437.1"
                     /translation="MFQTNGSCFITLNKAAGISSASALNRLKNLLKLKKAGFCGTLDP
                     LAKGVIVVATGKATKLISLVTDSDKIYSGKMILGYESASFDKDTELVPTGVEFDLSKV
                     DLREIEKKFTGYVKQTPPAYSAVKIDGVRAYKLARQGECPEIKERTVFMNRISLSVSS
                     DTEISFSIECSKGTYIRSLVNDIGKHIGCGAVMAELLRERVGGFDLAHSVTIDEIKND
                     ISSVERGVTEIDAFLKRFKTLDVDRNSYDWLRNGKDIAALKLELSEGLNYIRFENEPA
                     FVIKSEADSFSYFAYLRD"
     gene            11345..12175
                     /gene="folE2"
                     /locus_tag="PL056_05165"
     CDS             11345..12175
                     /gene="folE2"
                     /locus_tag="PL056_05165"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HPM46438.1"
                     /translation="MNDVQNYRDTRNIPVDQVGVWDIEYPVIVKDRANSTQNTVASID
                     MTVMLLPEFKGTHMSRMVEVLNSVRGEITMANMPGILKKMRDMLDSPQAITSMRFPYF
                     IEKKAPVSEISSMFPVSVLFSGFSDSKDGDSFILGVRTPVTTLCPCSKEISESGAHNQ
                     RSFVTLYLKFRDFVWIEDVVKIIEESASCEIFPLLKRNDEKFVTEKAFSNPVFAEDIV
                     RNVSVRMMKDSNVLWFMVETLHLESIHTHNAYARIERFRERSFLLDEHLRMFKGFPKI
                     "
     gene            12190..>13343
                     /gene="alr"
                     /locus_tag="PL056_05170"
     CDS             12190..>13343
                     /gene="alr"
                     /locus_tag="PL056_05170"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HPM46439.1"
                     /translation="MNNGAYTSWIEISKPAYKKNIEFFRSRIINGAEISVVIKANGYG
                     HGMLETAHIAQECGIEIFCVHSLEEALILRKAGFHTRIIILGPVMRETIPAVVENDLE
                     PVCYLIENLRLFEKHASIKDKSVNIHLKIETGTNRQGINGLELVEFVKELKKCDHVKL
                     KGVYSHFANIEDTTDRSYAKEQIDRFKREVELIRNAGFSGFDLHFASSAAAVIFQNTH
                     FSMIRLGISQYGLWPSKETYLSYITEHGHGPEHILTPVLTWKTVIGQIKNLKAGEFVG
                     YGCTHQVTRDSKIAILPIGYSDGYDRGLSNQGYVLVHGKRAAVVGRIAMNLTTIDITD
                     IPDVSVEDEVVLIGEQGDQIIRAENLAALTGTINYETVSRISSHIQRIVV"
BASE COUNT         4309 a         2418 c         3113 g         3503 t
ORIGIN      
        1 ccgattctcc gaaagtggct tttgacaaag ctgctgaatt tattacaaaa aaagagccga
       61 taagcattgc atatcacggt aatatcgttg atcttcttga atatgccgac aaaaacaact
      121 gccatattga catgctgagc gaccagacaa gctgtcacgc agtttatgac ggcggatact
      181 gccccgcagg tatcacattt gaagagcgga ccaaacttct tggaacagat aagcataaat
      241 ttaataaaat ggttgatgaa acgctccgca ggcattacga agtgataaaa aaattagtaa
      301 gtaaagggac ttactttttt gattacggca acagtttcat gagagcagtg tttgatgcag
      361 gagttaagga aatatcaaag aacggagttg acacatttga gggctttatc tggccaagct
      421 atgttgaaga tatcctcgga cctgaacttt ttgattacgg ttacggcccc ttcaggtggg
      481 tatgtctgag cggaaaagag gaagatctca taaaaaccga tgcggccgct gcggaagtta
      541 taaaaacctg tcgtgaagaa ctcaggtatc aggataaaga caactatcag tgggtaaaag
      601 acgcgatgaa aaacaaactc gttgtcggaa ctcaggcaag aattctctat caggatgctt
      661 ttggcagaac cagaatcgcc aagaaattca acgagatggt taaaaacggc gaaatcggtc
      721 ccatcatgct tggaagagat caccacgaca caggaggcac cgacagcccg taccgcgaaa
      781 catcaaacat caaagacgga tcgaacatca tggcagacat ggcgacaaac attttcgcag
      841 gaaactgtgc aaggggaatg agcttagttg cactccataa cggcggcgga gtcggcatcg
      901 gaaagtccat aaacggcgga ttcggaatgg ttcttgacgg ctcagcaaga gttgacgcaa
      961 tcctcgacat agcttttccc tgggatgtca tgggcggagt aagcagaaga gcatgggcaa
     1021 gaaatgaaca ctccatcgaa acctgtatcg aatataacga aatcaacaaa aacaacgatc
     1081 acatcacact tccctacatc gtaaaagatg aattaattaa gaaagtctgc aagttataat
     1141 taaaaatttg attttactca gtgcattgag taaaattgct cagttaattg agtaaaatat
     1201 tttatgtgga gtttgtgatg atcaaaagaa aggttttcaa cagaatattc aatatcttgt
     1261 gtgaatcgag gaagtttatt catgttttgg ctggaccgcg acaggtgggc aaaacgactg
     1321 tgataaatca ggtcattgag gaaataaata tcccttctgc atacttttct gctgacgatt
     1381 caacgcagac cgggaaattg tggatacaga tgaactggga ggcggcaaga ataaaggtca
     1441 gaacggaaaa gcttaacgat ttcatccttg tttttgatga aatacagaaa attcctgact
     1501 ggactgaaat gataaaaaaa ctatgggatg aagactcgtt atcaaaaatg ccgataaaag
     1561 tcgttctgct cggttcttca acacttttaa ttcagcaggg gcttaccgaa agtcttgcgg
     1621 gtcgttttga gataacccgt cttactcact ggtcgtatcc tgaaatggaa caagcgtttt
     1681 cgttttcagt tgagcagtat atttattttg gcggttatcc gggagctgca acgctgataa
     1741 atgatgaaga aagatggaaa aactatgtga gagattcaat tgtggaaaca accatctcca
     1801 aagatattct gctgatgaaa aggattgata aaccggcact tttaagacag cttttttatc
     1861 tcggtgcaag ttacagcggg caaatactct cattaaacaa aatgatggga cagcttcaag
     1921 atgccggaaa cacgacaact ctttcccatt acacagaact tcttgataaa gccggaatgg
     1981 tgaaaggatt gcagaaatat gcttctcaac aattcagaca acggaattca agtcctaaat
     2041 tcatcgttta tgacacagct tttctgactg ttcagcttgg aattagtttt gaaaaagcag
     2101 tttcagacag agcattgtgg ggtcgtgttg ctgaatcggc agtaggagca tatcttgtaa
     2161 atcaatgtgt cggaaaagac attgaacttt tttactggag agaaagccct gatgaagttg
     2221 attttgtttt gaaaaaaggg gaaaacattg tagcaatcga agtaaaaacc ggatttaaaa
     2281 atgaaagttt aaaagggatg agcaaattta ttcagagttt tcccaaatcc aaacccttac
     2341 tgatcggaac aggcggaatt cctgttgccg atttcttcaa aattgaaatt gaagagctgt
     2401 tttgatattc agatcaatcc ttatattctg caacttttct gcatgaattc tgataaataa
     2461 gttcaaacag gaaatagttg tcaggatttg tggtgacccc gaacaaatgt ccgtcccgca
     2521 tgatcttttc agttgccaaa ataaactctt cggagttaag gaacttttcg taataagtta
     2581 tgaaaacacc gatctcattg cggtttttaa gcgtattctc aatataaccg tcatagtcgc
     2641 cattcagata ttttgcgatc ttttcatttt ttatttcaaa accgctgtaa tcagctgaat
     2701 ttgttctctg attgatagtt ccgtaaagat caacgcaagg tccgaccagc agctttgtgt
     2761 ttttgaaccg ttcctttaaa aagtctatcc tttcagccca tagttcttca gaaatttcgt
     2821 tgaactgcgg acagtaacct aacatgtcaa tattactgat atcttcgctt ttaaggtttt
     2881 tgaggaattc aaatgaccac gaaccgaaaa atcgttcaaa tttatagccc agtttttctg
     2941 tatttttctc attcgtgatc cagtcaagat caggtttgtc accgacaaga ccgaacattt
     3001 tcggctttat cttttttatg tgcttcatcg tttcatcagt gggaatgccg gcgatgctga
     3061 tcccgtccgg aaaaatgcat gctttgccat tcagtttgtt ctttaccaga tttgagacca
     3121 tcattccggc aagtaattca ggattgtttt tctctgtaac aaaaaaagtt tcagcatttg
     3181 ctaaaaatat gtttccagcc ggctcaataa ttctgaaatc ttcataacat tcagcaaaat
     3241 acctctgaaa aggttgtgac tgatgttgtt tttcaattaa agcttttccc agttttgtgc
     3301 tttcagcaag gacaacagtt ttagcaaata ttttttcaat cgttcctgca aattcaacgg
     3361 tgatgacaac tcctccttca actggcaaca aattctgaat tgaggatccc cagaaaatat
     3421 ttttgttcat tttattgagt atttccagac ttttattgga ttcagcataa aaaaaagaat
     3481 ttgtctgcaa ttcatttata ataagcattg aatcatgctc caccgaaaaa aaagttttca
     3541 gtgccgtcgc atttcttccg attaccacaa gatctttttc aaataaatag tccgcagaaa
     3601 gggtttgtat gcttaaaaca aaaaacatga gaagcgttat tctcaaatcc ggctccatta
     3661 aacagattaa acataaaaat attactttcg tttttatatg tgtcaacaca aagatgtaac
     3721 cggctgaaag agagctttca attgacacag aaacacagct aatataaaaa aattgcttga
     3781 tttttatctt ttgcgtggta ttaaatgagc ggaaagttct ttttctaatt ttctctgcat
     3841 gcggtaaaac gcaagattaa atacagtgag gttgttaatg tggagtggca gagctaagga
     3901 tatttttaac aaattggagc cgttggcaaa cagcatgggg ctcaagatcg ttgaacttga
     3961 tcttcctatc ggccagaacg gtgttttcag gatatatctt gacacaatgt ctgctgacaa
     4021 aggaattacc gttgatgaat gcggcaggtt cagtccaatc gtcagcgact atcttgatac
     4081 tgaggatatg ttcccgttca gatactatct tgaagtaagt tctcctgggc ttgacagacc
     4141 ttacagaaga tgggaagacc ttcatcttgc gataggaaaa acactcaaaa taaaattatc
     4201 ggaaacagtt gaaggtagaa agcggataac cggaactctt atctcggtct ctgatgaaaa
     4261 aggggagttt acagttgatt cagaggggac gatgataacc attgcaaaag gatttgtgaa
     4321 aaaaataaat gagatttgga aaggagaaaa atgatggatt tcaatcttaa agagttggtt
     4381 gaccagctgg tcaagatgaa agggatcaaa cgtgaattaa ttgtcgacat tcttaaagag
     4441 gccctttatc aggcggtgaa gaagaaactc ggttccgatg ccgacattga tatcatgttc
     4501 gatgatgcga caggagagct gcaggcgttc tatttcagag aagttgtcga aggtgaaggt
     4561 gatcctcagc ttgacattaa tattgaagag gcgcgcaagc ttgatcctga agtgcagatc
     4621 ggagatacgc tcggagtcaa aatggaagcc gatgaacttg gaaggatcgc ggcacaggtt
     4681 gccaagcagg tcatagttca gaagataaaa actcttgaag gtgacataat atataacgaa
     4741 ttcaaggaca ggcagaatga aattatcacc ggtacggtca gaaggtttga aaaagggggt
     4801 ataatagttg atataggaaa aaccgaggca tatcttcctt acagtcagca cattccttcc
     4861 gacagattca gaattaacga caggatcaag gcattcataa cggaaattgt gaaaactccg
     4921 cagggatgca acatacttct aagccgtatc aacacaatgt tccttataaa actttttgaa
     4981 ctagaagtgc ctgaaatagc tgaaggaata gtcaaaatag aaggtgttgc aagagatccg
     5041 ggacaccgtt cgaagatcag tgtcagatca atagatcacg atgtcgatcc ggtcggagca
     5101 tgtgtcggaa tgaagggctc aagaattcag aatgtagttc atgaactcag tggcgaaaag
     5161 atagacatca ttccgtggga tgaagattct gtcagatata tctgcaacac tctttcccct
     5221 gttgaagttt cacagataat aattgatgaa gatgagcaca gcatggatgt aatagtccct
     5281 gacgatcagc tttcagtcgc aataggaaga ggcggtgaaa atgtccgtct tgcaagccag
     5341 cttaccggat ggagtattga tattcagagt gaaagccaga tggcaatgat gcttgaagag
     5401 gcgaagagac ggcttctttt cattgacggg atcgatgaaa gcatcgctga ttcgcttatt
     5461 aaacttgggt acacgactct tgaggacctt acttctgttg ctcctgaagt gttgtctgaa
     5521 cttcccgata ttacaacaga aaaagctgag tctataatca atcaggccaa aaagatgctc
     5581 gaaaagggaa tgggaatgat agatattgac gatgatcttg aggaaaaact caatgttccg
     5641 accacgacgc tcaaatgtgt caacgaagaa cttgctgaat atattgctga aaaagggtat
     5701 catacacttg cagatattca gagcgaaccc gatacggaaa cattcgccaa gaacgcagag
     5761 ctgagcttga gaagagcaag gcagataaga tatagtctgc agatccatat cgatgatctc
     5821 aatggaaaga aaagaggtaa ggacggcaga gtcaacgctg actcattctt tgatttcagc
     5881 cgtattgaag aaaacgggga agatgaaaag aactgacaga gaaagaacat gttttgcctg
     5941 taacgggacc gatagtaagg aaaatcttat gaaatgggtc gccgttgcag gaagaatatt
     6001 gccggactgg cttcagaaaa tagaagccag atctatatat acgcactaca gcaagaattg
     6061 catttcagct tttctaaaca gcagaaaact tcctgaaaag ttttttcatg ggatacctgt
     6121 ttttgagatc ggtcaggaaa gaatattcag ttttttgaaa gagaggtctg ttgattcgat
     6181 cagccatttt ctttcgatct gtagaaaaag cggggtcgtt ttcaaagggc agaatcttat
     6241 tatggaaaag gcaaagaagg ggcagaaatt taaatatctc atctgtgcct ccgatgtttc
     6301 tgaaaacaca gtttctgatg ttgaaagagt tacaggcatg cattgtttta agacaggact
     6361 tgacaaatct gctgtcgggg cgatgtttga cggcaggcca accggtgttt ttgcccttgt
     6421 tgaatcagct cagagtgaaa aactgttttt ttatatgaac ttattggaaa aattcatttc
     6481 aggagataaa tatgcgcatt agtctgctat ccaaagagat aggtgtagga agtaaggaga
     6541 tcatggagtt ccttaagtca aaaggtcttg attattcaag ccacttcaat gttcttaatc
     6601 cggatgagga aaagctggtg agagaaaaat tctcttcagg tttgcctgtt gctgaagccg
     6661 aagatgattc aaagaaaaca gttacaacaa taagaagatc aggtgctcag agacagagga
     6721 gttttattcc tcattcgcag cgtgaatccg ctgaagaagg tgctcttttt aaaaatcttg
     6781 atgaacaagt cgggacagat gatcaggaac atcctgtttc tgcagaaaat agaacatcag
     6841 aagaaaatga taatactgcg gataataaat ccaaaacaga tgaatccggt acaaacgata
     6901 aaaaatctga agagcatgtc catgaacacg aacatgacag aagacttcgt gttgaagagg
     6961 aggcgagaag acttcttgcg ggagactccg gaactgagga tgtaattgaa aaagtcgaga
     7021 aggatgaaaa ggatacttca aaaaaagatg ataaaaagaa aatcgaagtt cttgatgaga
     7081 ttatagatga tgatgatctg gatctcaaga gcaagcacaa gaaaagaaag gtcgtaattc
     7141 ctgataatga gacacctgtt gttgcaaata ttcctgctca gaggcacaac aaagtaagga
     7201 agaatatcaa aaatgtcgag ccggcaaatg tttcgctcaa ggttaaaggc aaaataaagg
     7261 gtccggacat tgcacagttc atcgaagatc catatgcgat tcagcgggag gcgcgcagaa
     7321 ggaagaggga tagaaggaaa atgaagcccg ttgcagttgt gccggctaat ccgacaaaag
     7381 cgataaaaaa agtattgaag ctcgaagata cagtgcaggt ttcagaactt gcaagaagaa
     7441 tgggtgtgaa ggcgtctcag ctcatttcaa agcttatggt catgggagag gtgaccggta
     7501 taaatgacag aataaactat gaaatggcac agcttcttgc ggaggagttc ggttttacag
     7561 ccgaaaatgt agctctaaat gaagaaacat acatagagat cagcgaagtt cgtgaggatc
     7621 atcttgtttc cagacctcct gttgtaacag ttatggggca tgtcgatcac ggcaaaacaa
     7681 aactgcttga tgcgataagg catacaaatg tggttgacgg tgaagcaggc ggaattactc
     7741 agcacatcgg agcatattca gtagaacttg aaagagggaa gataaccttt cttgatacac
     7801 ccggacacga agctttcacc gcaatgagaa gcagaggtgc acaggcgacg gatgttgtta
     7861 ttctcattgt tgcggctgat gacggcgtaa tgcctcagac agttgaggcg ataaaccacg
     7921 ccaaagcggc gaatgttccc atgatcattg caataaacaa aatagacaag ccggatgcaa
     7981 atccgcagaa ggtcaagaac gaacttctta aatatgagat cgttgctgaa gaactgggcg
     8041 gagaacatct tttcgtcgaa atatcggcaa aacagagaat aaacattgaa ggactgctcg
     8101 aatctgttct tcttcaggta gagatgatgg atcttaaagc cgacccgaaa aaaccggctg
     8161 aaggaatagt cattgaatca aaaatggatc cagggaaagg accggttgca acagtgcttg
     8221 tcaaagacgg gacattgaat atcggtgatc ttgttgttac aggaacatca atgggatttg
     8281 taagaagcat gatggactgg actggttcga gagttaagga tgcgggtcct tcaatggccg
     8341 ttgagataac aggtcttgac tttgttccgc cggcaggaga aaaactgcat gtgttcaaag
     8401 atgagaaaaa agccaagggt cttgttgatc tcagatgcaa caagcttcgt gaaaagaaac
     8461 atgaaaagga aagccggatg tcgcttgaaa atctttttac taaaatggaa ctgggtgatg
     8521 ttcaggacat aaatatcgtt gtaaaaggtg atgtagacgg ttctgttgaa gcgataatct
     8581 catccttgaa aaagatagag cacaagaaga tgaatgttaa gataatacat gccggtgtcg
     8641 gcggaataaa cgaaaacgat gttcttctgg caagtgcttc caacggtatt attgtcggat
     8701 tcaatgtcag aatagataac aaggcaaagc agcttgcggt ttcagaaatg gtagacatca
     8761 gaatattctc tattatttat gaactcattg attcaataaa gtctgcaatg acagggatgc
     8821 ttgctcctat aattaaagaa gagctttcag gcaaacttga ggtcagggat atatttaaag
     8881 tgggcaaagt tggaaccatt gccggatgta tggttgttga aggcaggata gtcagaggcg
     8941 gcaaggttaa acttgtcaga gaaaatgtcg ttatatatga aggtaaactc ggtacgctca
     9001 aacgattcaa agatgatgtt aaagaggtgc gtaacggtta cgaatgcggt gcaacgatag
     9061 acaacttcaa cgatatcaag gtcggtgatg tgatagagtg ctacattgat aaagagataa
     9121 gggacgaggt tgtctgatgg ctggacagat aaggcagagc agaatagcca ctgccattaa
     9181 aaagtttgta agtacgctca tgctcagcga atacggtgat tctcccgttt caatgataac
     9241 catcagcaaa gtaactgtaa tgcctgacct tagatcggca aagatattct attccacttt
     9301 cgatagcggg agtgtcgata agatacagga ataccttgat ctcaagacaa agaacataag
     9361 attcaagctg gccagtcacc tgaaaacatt gaagttcgct ccggagataa aattcgtcta
     9421 tgacgattcc gttgaaaaga tcatccggct cgaaaagatt tttgattcaa taaaatctga
     9481 tcagaacggc agcggtaatg atgagtgatt tcagaaaaat aaacgaaata attgacagct
     9541 cccgttcgat actgatcgcc gggcacgaaa tgcctgacgg tgacagcatg ggaagttgta
     9601 ttgcaatggc gtcgatcctt gaaagaatgg gaaaagatgt caaggtttac aggtcaggtc
     9661 agttcccgtt caactatatg tttttaaaag gttcaggtaa tgcatgtgag tcaattcctg
     9721 aaggaccttt cgatcttttt gtaatactag acagcggatc tccggagaga gtcggtgaag
     9781 atctttataa attaatggca tcatcaacag ggaagaaaat actttttgat catcatactc
     9841 tgaaaaaaga gaacgatgat ttttttgatg ctgtatattc cgacacttca gcttgtgcaa
     9901 ccgctgcaat tgtctacaga tgggctgcgg atagagggat ggctcttaac aaggaagagt
     9961 cggaagcggt gtattccgcc atcataagtg acaccggcgg attgaaatac ggcagcacca
    10021 acaaagaatc atttataatt ctttctgaac tgcttgaccg cgtcaaccca tgggatgttg
    10081 ctacaaggat atatgaaaat gttccggtgg aacagctgaa aatgctttcg gaagttcttg
    10141 ccgacctgcg aattcttgct gaaggaaagg cggcagtaat acggatcact cttgaccagt
    10201 ttgaaagata taaacttcat ccggaccatg ttgattcttt tgtcaactat gcaagaagta
    10261 taaaaggggt gcagatcgcc atgagattca gggagaaagg acccggcaca tggaaagtaa
    10321 gcatgagaag ccgcggaaac attgatgcaa atgaagtcgc atcaaggttt ggaggcggag
    10381 ggcataagaa tgccgccgga tttgttttta aaggtacttt tgaagaaggg ctcaaagcag
    10441 tagaggaaat tgtcagaagt gtttcaaact aacggatcct gtttcataac tctgaacaaa
    10501 gcggccggta tatcatccgc atccgccctt aaccggctca agaatctgct gaagttgaaa
    10561 aaagccggtt tctgcggcac gctggaccct cttgccaaag gagttatcgt cgttgcaacg
    10621 ggcaaagcta caaagctgat ctctcttgtg acagattccg acaagattta ctcagggaaa
    10681 atgattctcg gttatgaaag tgcaagcttt gataaagata cggaactggt tccgacaggt
    10741 gttgaatttg acctttcaaa ggtcgatctg agagagatcg aaaaaaagtt caccggatat
    10801 gtgaagcaga caccgcctgc ctacagtgcg gtaaaaatag acggggtaag ggcatataaa
    10861 cttgcaagac agggtgaatg tcccgaaatt aaggaaagaa cggtcttcat gaaccgcatc
    10921 tctctttcgg tttcatctga tacagaaatc tctttcagca ttgaatgttc aaaagggaca
    10981 tacatcagat ctttggttaa cgatatcgga aagcatatcg gctgcggagc tgttatggct
    11041 gaacttctga gagaaagggt gggtggcttt gatctggctc attcagtaac aattgatgaa
    11101 attaaaaatg atatttcctc cgttgaacgg ggtgtaacag agatagatgc tttcctgaaa
    11161 aggtttaaaa cgctggatgt tgacaggaat tcatatgact ggctgagaaa cggaaaggat
    11221 atagcggcac tcaagcttga gctttcagag ggtttgaact atatcaggtt tgaaaatgag
    11281 ccggcgtttg tcataaaaag tgaagccgac agtttttcct attttgctta tttgagggat
    11341 tgatatgaac gatgttcaga attaccgtga cacaaggaac attcctgtcg atcaggtcgg
    11401 tgtatgggat attgaatacc cggtaatcgt taaagacagg gctaactcaa ctcagaacac
    11461 agttgcttcc atagatatga cagtcatgct tctgcccgag ttcaaaggga cacacatgag
    11521 ccgcatggtg gaggtgctca acagtgtcag aggcgagatc acgatggcta atatgccagg
    11581 aatattaaag aaaatgaggg atatgcttga ttcgccccag gcgataacat caatgagatt
    11641 cccatatttt attgaaaaaa aagcacctgt ttcggaaatt tcttcaatgt tcccggtttc
    11701 cgttcttttc agcggattca gcgattcaaa ggacggtgac agtttcattc tcggtgtcag
    11761 aacgcctgtt acgacacttt gcccttgctc aaaggagata agcgaaagcg gagcgcacaa
    11821 ccagagaagt tttgtgacac tttatctgaa gttcagggat tttgtctgga ttgaggatgt
    11881 cgtgaaaata atagaagaat cggcaagttg cgagattttt ccccttttaa agagaaatga
    11941 tgagaagttc gtgaccgaga aagctttttc aaatcctgta tttgctgaag atattgtcag
    12001 gaatgtatct gtcagaatga tgaaagacag caatgttttg tggttcatgg ttgaaacact
    12061 ccatcttgag agcattcata ctcacaatgc ctatgcaagg atagaaaggt tcagggaaag
    12121 gtcatttctc cttgacgaac acttgagaat gttcaaaggt tttcctaaaa tttaaaagag
    12181 aggtgatttt tgaataacgg tgcatacaca agctggatcg aaatttcaaa accggcttat
    12241 aaaaagaaca ttgaattttt ccgttcaaga ataatcaacg gtgcagaaat atctgttgtt
    12301 ataaaagcaa acggatatgg ccatggaatg cttgagacag ctcacatcgc ccaggaatgc
    12361 ggaatagaga tattctgtgt acactctctt gaagaggcgt tgatacttag aaaagccggt
    12421 ttccatacca ggataataat actcggtcct gtgatgagag agacaattcc cgctgtagtt
    12481 gaaaatgatc tcgagccggt gtgttatctg atcgaaaatc tcagactttt tgaaaagcac
    12541 gcttctataa aggataagtc cgtcaacata catcttaaaa ttgagaccgg gaccaacaga
    12601 caggggatca atggtcttga actggttgaa tttgtgaaag aacttaaaaa atgtgaccat
    12661 gtaaagctga aaggggtcta cagccatttt gccaatattg aagatacaac cgaccgttca
    12721 tacgcaaaag aacagataga ccggttcaaa agagaagtgg aactgatcag gaatgcgggt
    12781 ttttccggat ttgaccttca ttttgcttca agtgccgctg cggtcatatt ccagaacacc
    12841 catttctcaa tgatccggct cggcatttca caatacggac tctggccttc caaagagaca
    12901 tatctcagtt atatcacaga gcacggtcac ggccccgaac atattctgac acctgtgctg
    12961 acatggaaga ccgtgatcgg tcagataaaa aatctcaaag caggcgagtt tgtcggatac
    13021 ggttgcactc atcaggtgac aagggattcg aaaatagcaa ttcttccgat aggttacagc
    13081 gacggatatg ataggggcct ttcaaatcag ggatatgttc tcgtacacgg gaaaagggcc
    13141 gcggttgtcg gcagaatagc aatgaacctc acaacaatag acataacaga catccccgat
    13201 gtctcagttg aagacgaagt tgtcctcatc ggagaacagg gtgatcagat catccgtgct
    13261 gaaaatcttg ccgcattgac cgggaccatc aactatgaaa cagtttcaag aatatcttcc
    13321 cacatacaga gaattgttgt tta
//
LOCUS       DAOOYB010000059        13338 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_4735_length_13338_cov_10.139803, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000059 DAOOYB010000000
VERSION     DAOOYB010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13338
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4735_length_13338_cov_10.139803"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="PL056_05175"
     CDS             complement(<1..356)
                     /locus_tag="PL056_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46440.1"
                     /translation="MKRSVFFTIISLFILIFVSCEQSKKIRIAPLQDEEMTESEDQDF
                     SDEDSGNTGDTGNTGNSGNSGNSGNSGNSGNTGDTGNSGDTGNSGNSGDSSTDEDIVV
                     IKDCTKDEDCVYLDGE"
     gene            498..2483
                     /locus_tag="PL056_05180"
     CDS             498..2483
                     /locus_tag="PL056_05180"
                     /inference="COORDINATES: protein motif:HMM:NF014466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HPM46441.1"
                     /translation="MKNYFLVLTLIMFGLVISGCLEKKTIKDKETYADSEIYDSDSVV
                     DDESTDGIINENDPVIDDIPEIPDDEDDDGWGGDDENPDEDDPRPDDVIINPDENGKP
                     DEDSIVEFSGTHFSYDYNGTKIIADMTALDGDNSIVLKKASLPSDLEGKVVITFKSDF
                     DEIVFSLSEEDLGNSDDITLDNTGNTAIWKVNGSVYGRFSGKVIIENFTKTGDAYSSV
                     KLNGNDLKFSNAVIENPDEDPDTEYPDQDEVPDIDPGDSSDLTFYQNGEDFSGSVSAE
                     TDESSFNFKASSKASIIFTSCPSDYCFSTTFSSRYGTINLRAAFNSNSFPSTIDLEDG
                     YSYLRWNTNGYQYGHFTGIIMVYDYAESGFPNFSVDLADIGSDSIYFIKDVVIEDSDG
                     DGIIDSADNCPSVSNPGQDDIDSDNIGDVCDDDKDGDGSLNNNDGCPADPLKTDPGTC
                     GCGVADTDSDEDGTPDCNDLCPDDINKIAEGICGCGISDSDSDNDGKVFCQDNCPNDF
                     NPDQTDTDSDGKGDACDTDIDGDGRNNSVDNCVYVPNSNQKDTDSDGIGDVCDNDIDG
                     DTILNENDNCPVNSNNDQLDSDEDGTGDVCDQCKNDPDKIIPGVCGCGAPDNDTDSDG
                     VLDCNDNCLNVFNKSQLDSDNDGIGDACDENPYSPDF"
     gene            2675..4522
                     /locus_tag="PL056_05185"
     CDS             2675..4522
                     /locus_tag="PL056_05185"
                     /inference="COORDINATES: protein motif:HMM:NF039815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46442.1"
                     /translation="MKSILFRLLLISTLVFTAACGPNDEENNQGDTGNSGNSGDSGDS
                     GNSGDSGNTGDTGDSGNTGNSGNSGNSGNSGNTGGEEEDACGPGLLKTEWETEAWKKA
                     DDDSDGIPNSVECSECPCNDTDGDGMPDYMDSDSDGDGYSDSQECPSQPCANSDKDDI
                     PDFIDKDSDNDGLSDKKEKEGGTDPYNKDSDGDGSDDLAEIAYGSDPLDDGDSIPAGI
                     FYVVLPYNAPEDVTRTLTFSTKIESIDVLIVFDDSGSMSDEQSNLKKEIKTKIIDAIA
                     TQFTSPGFAAYGFVIFGWDKNPYQILQHITINGDDVKSAVDSYKFGGQGDEFHWPSFY
                     LTATGEEYIGSVQSCLPAAFGGCNQNLVKDAVYNVAKADCTQKLGSVGGACFRKKTMP
                     IYIMISDEAFLDCIEFDPTFNKEGCMFNPGTKMLTREMAIAALNGIGAKFIGINSAFN
                     CDDQGNNCKVSNAPDEDYEFLSQMTGSLDGTGKNFNSHTANPDGSGMSDQIAQAIINL
                     TTFIDMDVTTGKMSDDDCSGQTAADFIKSSKTIAADPPEGVSGQSETTFFSVTQGTDV
                     TFDVRFFNDFCINSNDTYIKFDAQVTVLGNGSYLSSRLVNVIVPESNPK"
     gene            4545..6290
                     /locus_tag="PL056_05190"
     CDS             4545..6290
                     /locus_tag="PL056_05190"
                     /inference="COORDINATES: protein motif:HMM:NF039815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46443.1"
                     /translation="MKKSLVIFLALMIILSFQSCGPDDDNDDLGNSGDSGNSGDSGNS
                     GNSGDSGNTGDTGNSGNTGDTGNTGNSGECPCGAADEDDDGDGITNGVEGCIDSDGDG
                     LADCIDLDSDGDGFTDSEECPEQPCRDTDGDEIPDILDRDSDNDGLLDKKEKELGTDP
                     LKKDTDDDGNDDLAEVVYGSDPKDPEDSIPAGIFYVVLPYNGKTDVERELSFSTKIEA
                     IDVMFVIDGSGSMFAEIDQVRDNIKTQIIDTIKTEFPGNEFAAFGLSRITFANTGEFM
                     RQKMTLNTDQLKTALDNFGERQGGMAQGIAELHSPVLYWNANPEGFFGTASLTGFSIK
                     APINLAPADCTNDLGSIGHACFRKKSMPIYIYITDAEHADCGIAPNCSWDSAQELGPT
                     FDDALKMMGAIGAKFIGINTWHEFQDEEETKPADGANPIDDMTMMAQVTGSLDKDGNP
                     FIYQTVDNEGNGMPTQIGEAIVDLTTFIDMNVRTGKMSDEECNGTNAAEFIKSSKTVK
                     AVPPEGVDSFTDVEFISVKQGTEVFFNVKFYNDFCENTTEEPLEFEALVTVLGNGSFL
                     SSRIVTVIVPVNANK"
     gene            complement(6295..7806)
                     /gene="crtI"
                     /locus_tag="PL056_05195"
     CDS             complement(6295..7806)
                     /gene="crtI"
                     /locus_tag="PL056_05195"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019866844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HPM46444.1"
                     /translation="MKKHVIVIGAGPGGLTSAMILAKRGFDVTVFEKDDQVGGRNKEI
                     RLGEYSFDTGPTFLMLKNVLDEVFMEAGADGEKLLNYQKIEPMYRLVYDEKDSINPSS
                     DHEFMKSEIKRLFPGQEKGMEEYLKREKTRFEKMFACLQKPYGSITDYFSKQFINAIP
                     HFSLGKSLMDVLTGYFNEEKAALAFTFQAKYLGMSPWECPGAFALISYIEHAYGIYHV
                     EGGLCKISHAMADVAVKNGAKLHLSTPVKKVITENGKAVGVELENGEKTMADEVIINA
                     DFGYAMTHLFDEGVLKKYTPQKVRSMKYSCSIFMLYLGIDKVYDMPHHAIYFADDYRT
                     NVDDIFKNLKLSDDMSFYVRNTEGVDKTNAPEGHSALYILVPVANLEADVDWEKEKER
                     YTELVIDTVIKKTVMKDLKDHIKAQKVITPLDWQNDYNVYNGATFNLAHNLGQMLYSR
                     PHNQFEDVENVYLTGGGTHPGSGLPTIYESGRISANMICKKYGVKYESMNTLV"
     gene            complement(7834..9198)
                     /locus_tag="PL056_05200"
     CDS             complement(7834..9198)
                     /locus_tag="PL056_05200"
                     /inference="COORDINATES: protein motif:HMM:NF037833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46445.1"
                     /translation="MKSFVIVSVVLLALLNSAGCENKKKDTIYDDGNTGNSGDSGDSG
                     DSGDTGLPDNNTSDDDDIVKKDDDTVMSEDSDLFQNGDNENDEETVDVDSSDDIAVPD
                     EDAEKNDDGGRVMTAVILLPGYSSIHEAGKPFQIKGEVSDTKYPPDQLNVKWEIKKYS
                     GTWTTLVEDKPDSSGITEFQHTIPENDAAYFALRLTLKAPDDTEFMSQEKVFGICTYP
                     FGKIQDFNDPVLGEGWMLSGDAYRDSDGSNGWLELTGDDYNKRGVIYNNLMKVYPGDI
                     TIKLDIRTGQPSVPGDSGADGFAMTVFDSENAVALADYVAAVKTGGCLGYGVAGEYCG
                     LGGETIVDAFHIEFDTWYNSEQGLDDPTTENHIAVNLNGDPGGHQLWKNIFLEDSLWH
                     SIEVTTSGSKVTVKMDGNTLIDNDIEGFSFRGGYIVFSGSTGAAKNLHSVDDLEIVQE
                     CRVP"
     gene            complement(9296..10195)
                     /locus_tag="PL056_05205"
     CDS             complement(9296..10195)
                     /locus_tag="PL056_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HPM46446.1"
                     /translation="MAIIITKENRVIVQGITGREGQVRTKLMREYGSNVVGGVTPGRG
                     GETVMDLPVFDTVEELVENIGAVDTSVIFVPAHLVKDAAIEAILAGVKLLVIVPDRVP
                     VHDVIEIAAYAKKYGADFIGPNTLGVLSVDEGILGMIGGKASSAKAWFKKGNVGVTSR
                     SGGITSSISYYLSQSGVGLSTIVHVGGDGVIGMPHNRVMELFENDPQTELIVMFGEIG
                     TSQEEAVAEMMKAGKLTKPVVAYIGGKGAKKGTRFSHAGAIVEGNKGSWEGKVAALRE
                     AGAHIVENFQEIPAKVLEVLGKK"
     gene            complement(10198..11751)
                     /locus_tag="PL056_05210"
     CDS             complement(10198..11751)
                     /locus_tag="PL056_05210"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HPM46447.1"
                     /translation="MAKLYEYQGKQILKSVKIPIPEGGVAGSVDEAVKQANSIGYPVV
                     VKIQTWSTGRNAVGGVKFADNDLQLKEHVTRMLGMKIGNFAVEKVLVEKKIAIKREFF
                     AGFILNDTDKCPMLLFSSRGGSGIEEIAKEYPESVATMKIDFFNGFKPFEAISLVKKT
                     GVDSAGAAEIGPVLVKMYEGMKKSDARSFEVNPLVQLEDGKIMAADCHVVIDDYAVYR
                     HPEFGIEIAREFDRPPTELERIAYMVEKNDYRGTFFFFQMNEANEFENVIGFHGAGGG
                     GSMMSMNAVSENNCKIANFCDTSGNPPASKVYRAAKVILSQPGIKGYFASGSGVASQE
                     QFHSARGFVKAFLEEDIDIPCSIRLGGNSEDLAIEILEQYLGNKSVNIKGFGKNDSPD
                     KVMKHLLEGMEKNGGAGHKLTPFVLPDVSKCDYSFQTMTGSIHVETAKCETCTSKACI
                     ESCIPKILKDENGSPRLAIDIKEAKKGKCIECLACELACMSKGNQALKIVLPIEGLKE
                     YREKISEGK"
     gene            complement(11870..>13338)
                     /locus_tag="PL056_05215"
     CDS             complement(11870..>13338)
                     /locus_tag="PL056_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46448.1"
                     /translation="KYFWILIAAFMFVPVFADTDIEPDDGDVEVTDNYADEEELPDED
                     FSLEEDGAGLSITTAVNGSTVIYGRVFYNYITDYSRGRIYIYEQIGETGEFAFAGQIP
                     ANREKTSYFGGFVGISQNTLFTLNAIYGNTTFNDKQLDIYRKNDDSAWLHHKTVELPE
                     NYDVTGKGFATDNGNVLVAAYNTTIDADSSEIFVYSDADDYTSHKTLFPANVDKNLHR
                     FFFPGGLDISGDTIIATSTSLETEYQAGPTSIHIFSKDPESGEWSETQTVTSEFGETV
                     KAYYNISISGERFAVQTFLDEELTQGSVQIFEKLEGTWELVKEILPPEDAKNHFGYNV
                     SMGEDFAVATDKGNSKSLPYGTTYVFYKDYDPANPDTKTEDNWGLFDKYEVTDESVTP
                     LMGSVAVIDGDTLSMGAAPTILKAGDPMTPYIAKLKTPEFTETPDETETPDETEVNDE
                     KETTDDAKTDDSDSAEETTDTESKDKEEKSSGCSVMVF"
BASE COUNT         3727 a         2999 c         2701 g         3911 t
ORIGIN      
        1 cattctccat ctaaatatac acaatcctcg tcctttgtgc aatccttgat gacgacaata
       61 tcttcatcag tgctgctgtc acccgaattt ccactgtttc ccgtatcacc tgaatttccg
      121 gtatcaccag tgttaccgga attgccactg ttccctgaat tccctgaatt ccctgaattc
      181 cctgtatttc ctgtatcacc cgtattcccc gaatcttcat ctgagaaatc ctgatcttca
      241 gattcagtca tctcttcatc ctgtaaagga gctatcctga tcttcttcga ctgttcacag
      301 gaaacgaaaa tgagaataaa aagagaaata attgtaaaaa aaactgacct tttcatgttt
      361 ttctccgttg tgataaacat tcaacacaaa ccattttatc ctctgttgtc tcaaaggtca
      421 acagatttga cttaaaaata aaatacagta acattctttc cagttttggt gatttttaca
      481 ttcttatgag gtgagttatg aaaaattatt ttctggttct tacattgatc atgttcgggc
      541 ttgtcatttc aggatgtctt gaaaaaaaga ccataaaaga caaagaaacc tatgctgatt
      601 ctgagatcta tgactctgat tcagtagtcg atgatgaatc gaccgacggc atcataaacg
      661 aaaatgatcc tgttatagac gacatccccg aaattccgga tgacgaagat gatgacggat
      721 ggggcggcga tgatgaaaat cctgacgaag atgatccaag accggatgat gttataatca
      781 atcctgatga aaatggaaaa cctgatgaag attcgattgt ggaattcagc ggaacccatt
      841 tttcatacga ttacaacggc acaaaaatca ttgccgacat gacggccctc gacggagaca
      901 attccattgt tttgaaaaaa gcatctcttc catcagatct tgaaggaaag gttgtaataa
      961 cattcaaaag cgattttgat gaaatcgtgt tttccttatc cgaagaagac ctcggcaact
     1021 ctgatgacat cacacttgac aacactggga acactgccat ttggaaggta aacggttcag
     1081 tttacggccg tttctcgggt aaagtaatta ttgagaattt tacaaagacc ggcgatgcat
     1141 attcttctgt gaagctaaat gggaacgatc tgaaattctc aaacgctgta attgaaaacc
     1201 ctgatgaaga tcctgacacg gaatatcctg atcaggacga agttcccgac atagatcctg
     1261 gcgacagttc cgaccttaca ttctatcaga acggagaaga tttttcaggt tccgtaagtg
     1321 cggaaactga cgaaagttca tttaatttca aagcttcttc aaaagcatcg atcattttca
     1381 cgagctgtcc ttctgattac tgtttttcca caactttttc aagcagatac ggaacgataa
     1441 atctcagagc ggcattcaac agcaactcct tcccttcaac tatagatctt gaagacggat
     1501 attcttattt gagatggaat acaaacggat atcagtacgg tcacttcaca ggtatcataa
     1561 tggtatatga ctatgctgaa agcggatttc caaatttttc agttgatctt gcagacatag
     1621 gttctgattc aatttacttc atcaaagatg ttgtaataga agattccgac ggtgacggaa
     1681 taatcgattc tgcagacaac tgtccttctg tttccaatcc gggtcaggat gacattgatt
     1741 ccgacaatat tggagatgtg tgtgatgacg acaaggacgg tgacggaagt ttgaataata
     1801 atgacggatg ccctgccgat cctttgaaaa cagatccagg cacctgcgga tgcggagttg
     1861 ccgacaccga ttctgatgaa gacggaactc cggactgcaa cgatctttgt cctgacgata
     1921 taaacaagat cgctgaggga atctgcggat gcggaataag tgacagcgac agtgacaatg
     1981 acggcaaggt tttctgtcag gacaactgcc cgaatgattt caatcctgat cagaccgata
     2041 ccgattctga cggcaaaggc gatgcctgtg ataccgatat tgacggtgac ggacggaaca
     2101 attcggttga caattgtgtc tatgttccga actctaacca gaaagatacc gattctgacg
     2161 ggattggaga tgtctgcgat aatgatattg acggcgacac gatcctgaat gaaaacgata
     2221 actgtcctgt gaattccaac aatgatcagc ttgatagtga tgaagatgga accggtgatg
     2281 tctgcgacca gtgcaagaac gatcctgata aaatcattcc aggagtttgc ggctgcggag
     2341 ctcctgacaa cgacacagat agtgacggag tccttgactg caacgataac tgcctgaatg
     2401 ttttcaacaa atcacaactt gacagtgata atgacggcat aggtgacgcc tgtgacgaaa
     2461 atccctactc tcccgatttt tagttcttag aaactcctga aatcattaaa gccgtgtctg
     2521 ttagtatttc acgctaataa caaaatcctc aattttttca ataattttaa acttttctct
     2581 tcattttcgc atctattttc ttataatcag aaaagttgat gttgtgtgac actaaaattt
     2641 agtagtattt gacaataaac ctcaggaggg aacgatgaag tcaattttat tcagattatt
     2701 actgatttca actctggttt ttactgctgc atgcggacca aatgatgaag agaacaatca
     2761 gggtgacaca ggtaacagcg gaaacagtgg tgattcaggc gatagcggta attccggtga
     2821 ttcagggaac acaggcgata ccggagactc aggaaataca ggtaattcag gaaacagcgg
     2881 aaactccggc aactccggga acaccggcgg tgaagaggaa gatgcctgcg gaccgggact
     2941 gctcaagacc gaatgggaaa cagaggcctg gaaaaaagct gatgatgata gtgacggaat
     3001 acccaattcc gttgaatgtt cagagtgtcc ctgcaatgat accgacggtg acggaatgcc
     3061 cgattatatg gattctgaca gtgacggtga cggatacagt gactcgcagg aatgtccatc
     3121 tcagccctgt gcaaactcag acaaggatga tattcccgac tttattgaca aggatagcga
     3181 caatgacggt ctaagcgata aaaaagaaaa ggaaggtgga acagatcctt acaacaagga
     3241 ttccgacggt gacggaagtg acgatcttgc cgagatagct tatggctctg atccgcttga
     3301 cgacggggat tcaattcctg ccggcatttt ctatgttgtc cttccatata acgctcctga
     3361 agatgttaca agaaccctta ctttttcgac taaaattgaa tcgattgatg tattgatcgt
     3421 ttttgatgat tccggctcaa tgagcgatga acagagcaat ctcaaaaaag agataaagac
     3481 taagatcatc gatgctattg caactcagtt cacttcaccg ggatttgcgg cgtacggatt
     3541 tgtaatcttc ggatgggata aaaatccata tcagatcctt cagcatatta caatcaatgg
     3601 tgatgatgta aaatccgctg ttgacagcta taaattcgga ggacagggtg atgagttcca
     3661 ctggccgtca ttctacctta cagcgacagg agaggaatat atcgggtcag ttcagagctg
     3721 tcttcctgcg gcttttggcg gatgtaatca gaatttagtt aaagatgctg tatacaatgt
     3781 tgcaaaagct gactgcaccc aaaagctcgg aagtgtcggc ggagcatgtt tcagaaaaaa
     3841 gaccatgcct atctacatca tgataagcga tgaggcattc cttgactgca ttgaattcga
     3901 tcccactttc aacaaggaag gatgcatgtt caatcccgga acaaaaatgt taacccgtga
     3961 aatggcaatt gccgccctta acggcatcgg tgcaaaattc atcgggataa actctgcttt
     4021 caattgtgat gatcagggaa acaactgcaa ggtcagcaat gctccggatg aagactatga
     4081 atttctttcc cagatgaccg ggtctcttga cggaacagga aagaacttta attcgcacac
     4141 tgcaaatccg gatggatcag gcatgagtga tcagatcgct caggcaataa taaaccttac
     4201 aacttttata gacatggatg taacaaccgg aaaaatgagc gatgatgact gtagcggcca
     4261 gacagcagcc gatttcatca aatcttcaaa aaccattgcg gcagatccgc cggaaggtgt
     4321 ttcgggacag agtgagacca ctttcttttc tgtaactcag ggaacagatg taacctttga
     4381 tgtcagattc tttaacgatt tctgcatcaa cagcaatgat acctacataa aattcgatgc
     4441 tcaagtgact gttcttggca acggcagtta tctttcaagc cggcttgtaa atgtaatcgt
     4501 tcctgaaagc aaccctaaat aataattagg atatggagaa tacaatgaaa aaatctctcg
     4561 taatttttct ggctcttatg atcattttaa gtttccagtc atgcggaccg gatgatgata
     4621 atgatgatct tggaaattca ggtgattcag gtaattcagg tgattcaggt aattcgggaa
     4681 attcaggtga ttcagggaac acaggcgaca ccggcaattc cggtaataca ggcgacacag
     4741 ggaacaccgg caacagtggt gaatgtccct gcggtgcggc tgatgaagat gatgacggtg
     4801 acgggataac aaatggcgtc gaaggttgta tagacagtga tggagacggc cttgcagact
     4861 gtattgatct cgactccgac ggagacggtt ttaccgattc tgaagaatgc cctgagcagc
     4921 cgtgcaggga tacagacgga gatgaaattc ctgacattct ggatagagac agtgataacg
     4981 acggactgct tgacaaaaag gaaaaggaac tgggaacaga tcctctcaaa aaagataccg
     5041 atgacgacgg aaatgatgac cttgcggaag ttgtttacgg ttcagaccct aaagatccag
     5101 aagacagtat tccggcagga attttctatg tcgttcttcc atataacgga aaaacagatg
     5161 ttgaacggga attgtcattc tcaacaaaaa tagaagcgat tgatgttatg tttgttattg
     5221 acggttcagg cagtatgttt gctgaaatag atcaagttag ggataacata aaaactcaga
     5281 tcattgacac cataaaaact gaattccctg gtaatgaatt tgccgctttc ggcctttcaa
     5341 ggataacttt tgcaaatacc ggtgaattca tgcggcagaa aatgacactt aacacagatc
     5401 aacttaaaac tgctcttgac aactttggcg aaagacaggg tggaatggct cagggaattg
     5461 cggaactcca cagtcctgta ctttactgga acgcaaatcc tgaaggtttt ttcgggactg
     5521 caagtcttac aggattttca attaaagctc ctataaatct tgctcctgct gattgtacta
     5581 atgatctggg atctatcgga cacgcatgtt tcaggaaaaa atcgatgccg atatatattt
     5641 acataaccga tgcagaacat gctgactgcg gaattgcacc caactgttca tgggattcag
     5701 ctcaggagct tgggcccacc tttgacgatg ctctgaaaat gatgggggcg atcggtgcaa
     5761 aattcatagg gataaacaca tggcatgaat ttcaggatga agaagaaact aaaccggctg
     5821 acggagcgaa ccccattgat gacatgacta tgatggctca ggttaccggc tctcttgata
     5881 aggatggcaa tccgttcatt tatcagaccg tggacaatga agggaacgga atgccgacac
     5941 agatcggtga ggcgatagtt gatctgacca cttttattga tatgaatgtc agaaccggaa
     6001 aaatgagtga tgaggaatgc aacgggacaa atgcagctga gttcataaag tcatcaaaaa
     6061 ccgttaaagc agttcctcct gaaggtgttg acagctttac cgatgttgaa tttatttccg
     6121 taaaacaggg aactgaagtg tttttcaatg ttaaatttta taatgacttc tgtgagaaca
     6181 caacagaaga accacttgaa tttgaagcac ttgtcacagt tcttggcaac ggatcattcc
     6241 tttcaagcag gatcgttaca gtaatcgtcc ctgtgaacgc caacaaataa agatctacac
     6301 cagagtattc attgattcgt atttgacacc gtattttttg cagatcatgt ttgcagatat
     6361 tctccctgat tcatagatag tgggaagtcc gcttccgggg tgtgttccgc cgcctgtgag
     6421 atatacattt tcaacatcct caaactggtt atgcggtctt gaataaagca tctgtccaag
     6481 attgtgcgca aggttgaaag tcgctccgtt atagacattg taatcgttct gccagtcaag
     6541 aggagttatc actttctgtg cctttatatg atctttgaga tctttcataa ctgtcttttt
     6601 tataactgtg tcaatgacaa gctctgtata tctttccttc tctttttccc agtcaacatc
     6661 agcttcaagg tttgcaactg gaacaaggat ataaagagcg ctgtgccctt ccggagcgtt
     6721 ggttttatct actccttcag tgtttctaac ataaaacgac atatcatcgc tgagtttgag
     6781 attcttaaag atgtcatcga cattggtcct gtagtcatct gcaaaataaa ttgcatgatg
     6841 cggcatgtca taaaccttgt ctattccaag atacagcatg aagatcgaac aggagtattt
     6901 cattgatctt actttctgcg gagtatattt ctttaacact ccttcatcaa aaagatgagt
     6961 catggcatag ccgaaatcgg catttatgat gacttcatca gccatcgtct tctcaccgtt
     7021 ttcaagttca actccgactg ccttgccatt ttcagtgata acttttttaa caggtgttga
     7081 aagatgcagc tttgcaccat tcttaactgc aacatcggcc attgcatgag aaattttaca
     7141 aagccctccc tcgacatgat aaattccgta agcgtgttcg atgtatgata tcagagcaaa
     7201 ggccccgggg cattcccatg gtgacattcc gagatacttt gcctggaatg tgaaggcaag
     7261 ggcggctttt tcttcattaa aataaccggt aagaacatcc atcagagatt taccgagcga
     7321 aaaatgggga attgcattga taaactgctt tgagaagtaa tctgttattg atccgtacgg
     7381 cttctgaaga catgcgaaca tcttttcaaa ccttgtcttt tcgcgcttta aatattcttc
     7441 cattcctttt tcctgacctg gaaaaagtct tttgatctca cttttcataa attcatggtc
     7501 acttgatgga tttattgaat ctttttcatc atatacgagc cggtacatcg gctcgatctt
     7561 ctgatagttc aacagttttt caccatcagc tccggcttcc ataaaaactt catcaagaac
     7621 attcttcagc atgagaaatg tggggccagt gtcaaaagag tattctccaa gccggatctc
     7681 tttgttcctg ccaccgactt gatcatcctt ctcaaaaacg gtcacatcga atcctctttt
     7741 cgcaaggatc atagcgcttg taagaccacc cggtcctgca ccgataacaa ttacatgctt
     7801 tttcatttct tactccctga taacattaaa tatttacggc actctgcatt cctgaacaat
     7861 ttcaagatca tcaactgaat gaaggttttt tgcagccccc gtcgaaccgc tgaaaacaat
     7921 gtatccccct ctgaatgaaa aaccttcaat gtcgttatct atcaaggtgt tcccatccat
     7981 tttgactgta actttgcttc ctgatgttgt gacttctatg ctgtgccaca agctgtcctc
     8041 aagaaaaatg ttcttccaga gttgatgtcc ccccggatct ccgtttaagt ttactgcaat
     8101 atgattttca gttgtcggat cgtcaagacc ctgttcgctg ttataccatg tgtcaaactc
     8161 gatatggaaa gcatcaacta tcgtctctcc tccgagtccg caatattcac ctgccactcc
     8221 gtatccgaga cagccgccgg ttttcacagc ggcgacataa tcggcaagtg caacagcatt
     8281 ttctgaatca aagacagtca tcgcaaatcc gtctgctcct gaatccccag gaactgaagg
     8341 ttgaccagtc ctgatatcca gttttatagt tatgtcaccg gggtacactt tcatcagatt
     8401 gttgtatata actcctcttt tgttgtaatc atctcctgtc agttcaagcc acccgtttga
     8461 accgtctgaa tcgcggtatg catctccgga cagcatccat ccttcgccaa gaacaggatc
     8521 attaaaatcc tgaatctttc caaaaggata tgtacatatt ccaaaaactt tttcctgtga
     8581 catgaattct gtgtcatcgg gtgcttttaa tgtaaggcgt aatgcaaaat atgcggcatc
     8641 attttcaggg atagtgtgct gaaattcggt tatacctgaa ctgtccggct tatcttccac
     8701 cagagttgtc catgttcctg aatatttctt tatctcccat tttacattca actgatccgg
     8761 cggatatttt gtgtcactca cttcaccttt tatctggaaa ggtttccctg cttcgtggat
     8821 tgaagaatat cccggaagaa ggatcacggc tgtcatcact ctccctccgt catcgttctt
     8881 ttcagcatct tcgtcaggaa ctgcaatatc atcactgctg tcaacatcaa ctgtctcttc
     8941 atcattttca ttatcaccat tctgaaaaag atctgaatct tcagacatca cagtatcatc
     9001 atccttttta acaatatcgt catcatcaga agtgttattg tccggaagcc ctgtatctcc
     9061 gctatctcct gaatcaccgc tatccccgct gtttccagtg tttccgtcgt cataaattgt
     9121 atcttttttc ttgttctcgc aacctgcaga attaagcaaa gcaagaagaa cgactgacac
     9181 tatcacaaaa cttttcataa acaccccttt actctttcac taacaacaaa gaattatatc
     9241 agtgacttgg tttatgtcaa aaaagtggaa tgactattct taaaaaattg tgatcttatt
     9301 tctttccgag aacttcgaga acttttgcag gtatttcctg aaaattttca actatgtgtg
     9361 ctccggcctc cctgagtgca gccaccttgc cttcccatga acccttattt ccttcgacta
     9421 ttgctccggc atgtgagaat cttgtccctt tctttgcccc ttttccgccg atatatgcaa
     9481 caaccggctt tgtgagtttt cctgctttca tcatttcggc aaccgcctct tcctgcgaag
     9541 ttccgatctc tccgaacatt acgataagct ctgtctgcgg atcgttctca aaaagctcca
     9601 tcactctgtt gtggggcatt ccgataactc cgtctccacc gacatgaacg atggttgaaa
     9661 gtcctactcc cgattgagaa agatagtaac ttattgaact tgtgatacca ccgcttctgc
     9721 ttgtgacacc gacattaccc tttttaaacc atgctttcgc tgaagatgcc tttccgccta
     9781 tcattccgag tatcccttca tcgactgaaa gaactcccag cgtgttcgga ccgataaaat
     9841 ccgctccgta ttttttcgca taagccgcaa tttcaatgac atcgtgcact ggaaccctgt
     9901 ccggaactat cacaagaagc ttaactccgg caaggatcgc ttctatcgcc gcatctttga
     9961 ccagatgtgc cggaacaaaa atgacggaag tatcaactgc gccgatgttt tcaacaagtt
    10021 cttccactgt atcaaaaaca ggaaggtcca ttactgtctc ccctcctctt ccgggagtga
    10081 caccgcccac cacattggag ccgtattctc tcataagctt tgttctgacc tgaccttcac
    10141 gacctgtgat cccctgaact ataacgcggt tttcttttgt aattattatt gccatgacta
    10201 tttcccctct gaaatttttt cacggtattc tttcagtcct tcgatcggta gaactatttt
    10261 cagcgcctga tttccttttg acatgcatgc aagttcacat gcaagacact caatacactt
    10321 tcctttcttt gcttctttta tgtcaatggc aagccgtggc gaaccgttct cgtctttcag
    10381 tattttcgga atacagcttt cgatacacgc ttttgaagta catgtttcgc attttgcagt
    10441 ttccacatga atgcttccgg tcatagtctg gaacgaatag tcgcattttg aaacatcagg
    10501 aagaacaaac ggggtaagtt tatggcctgc tccgccattt ttttccatcc cttcaagaag
    10561 gtgtttcata actttatctg gagaatcgtt cttgccgaaa ccctttatat ttactgattt
    10621 atttccaaga tactgttcaa ggatctcaat tgccagatcc tctgaattac cgcctagccg
    10681 gatcgagcat ggaatatcaa tatcctcttc aaggaatgcc tttacaaaac ctcttgccga
    10741 atggaactgc tcttgagaag cgacgccgct tcctgaagcg aaatatcctt taattccggg
    10801 ctgactgagt atcacttttg cagctctgta aacttttgaa gcaggaggat ttcctgatgt
    10861 gtcacagaaa tttgctatct tgcagttatt ttccgaaacc gcattcatcg acatcattga
    10921 accgccgccg cctgcaccgt gaaaaccgat cacattctca aattcgttcg cttcattcat
    10981 ctggaaaaag aaaaatgtcc cgcggtagtc gttcttttca accatatatg cgatccgttc
    11041 aagctcagta ggcggtctgt caaattctct ggctatttcg atgccgaact caggatgtct
    11101 gtaaacggca taatcatcaa taacaacatg acagtcagcc gccatgatct tgccatcttc
    11161 aagctggaca agaggattta cttcaaatga tcttgcatca ctcttcttca tcccttcata
    11221 catcttgact aataccggac cgatttcagc cgcaccggca gagtcaacac ccgtcttttt
    11281 cacaagcgat attgcctcaa agggcttaaa tccattgaag aaatctattt tcatcgttgc
    11341 aacagattcc ggatattctt ttgcgatctc ctctattccg cttccgccgc gggaactgaa
    11401 aaggagcatc gggcatttat ctgtgtcgtt aagaataaat ccggcaaaaa actctctttt
    11461 tatcgcgatc ttcttttcaa caagaacctt ttcaaccgca aaattaccga tcttcattcc
    11521 aagcattctt gtcacatgct ctttaagctg cagatcattg tcagcgaact tcactccacc
    11581 cactgcattt cttccagttg accaggtctg aatctttaca acaacaggat aaccaattga
    11641 atttgcctgc tttactgctt catcaacact tccagccaca ccaccttcag ggatcgggat
    11701 cttaacactt ttaagtatct gcttgccctg atactcatac aatttagcca tgatcagtca
    11761 cctcacacag ttaatcaaca aaagcgaatt atcctaactc aaaaaatttt agattacaaa
    11821 ttttaagact tactgtctgc cttcgttgta gatctttctc caagtcatct taaaatacca
    11881 tcactgagca gccggaactt ttttcttcct tatctttgct ttctgtatca gttgtctctt
    11941 ccgcagaatc gctgtcatct gtttttgcat catctgtcgt ttctttttcg tcatttactt
    12001 cagtttcgtc aggagtttct gtttcatcgg gggtttcagt gaattcgggt gttttaagtt
    12061 ttgcaatgta aggggtcatg gggtcacctg ctttcaggat cgtcggggcg gctcccattg
    12121 aaagtgtgtc accgtcaatg actgcaactg aacccatgag cggagtcaca gattcgtcag
    12181 taacttcata tttatcaaac agtccccagt tgtcttctgt ctttgtatca gggtttgcag
    12241 ggtcgtaatc tttgtaaaaa acataagttg tgccgtatgg aagtgatttg gagttgcctt
    12301 tgtcagttgc aaccgcaaaa tcttctccca tggaaacatt gtatccgaaa tggttctttg
    12361 catcttcagg cggaagaatt tccttcacaa gttcccatgt cccttccaac ttttcaaata
    12421 tctggactga accctgtgta agctcttcgt caagaaatgt ctgaacagcg aatctttctc
    12481 ctgatatgga gatgttgtaa tacgctttga cagtttctcc aaattctgat gtaactgtct
    12541 gggtttccga ccactctccg ctttcaggat ctttgctgaa tatgtgtatc gatgtcgggc
    12601 ctgcctgata ttccgtttca agtgatgtgg aggttgcgat tattgtgtcg cctgatatat
    12661 ccaaacctcc cgggaaaaag aatctgtgca ggttcttgtc aacatttgca gggaaaagtg
    12721 ttttatgcga agtgtaatca tctgcatccg aataaacgaa tatttcactg ctgtcggcat
    12781 ctatggttgt gttatatgcg gcaacaagga catttccgtt atctgtggca aaacccttgc
    12841 cggtaacatc atagttttca ggcagttcga ctgttttatg gtggagccat gcggaatcat
    12901 cgtttttcct gtagatatca agctgtttgt cattaaatgt cgtattgccg taaattgcat
    12961 tcagggtgaa taatgtgttc tgagatattc cgacaaatcc gccgaaatag cttgttttct
    13021 ccctgttcgc cgggatctgg cctgcaaagg caaattcacc ggtttcacca atctgttcat
    13081 agatataaat tctgccgcgg gaatagtcgg ttatgtagtt atagaaaact cttccataaa
    13141 taaccgtgct gccgtttacc gcagtggtaa tcgatagacc ggctccgtct tcttccagtg
    13201 aaaaatcctc atcgggaagt tcctcttcgt cagcataatt atcagttact tcaacatcac
    13261 cgtcatccgg ttcaatgtcg gtatctgcaa aaaccggaac aaacatgaaa gctgcaatca
    13321 agatccaaaa atacttca
//
LOCUS       DAOOYB010000060        13334 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_4737_length_13334_cov_9.283304, whole genome shotgun sequence.
ACCESSION   DAOOYB010000060 DAOOYB010000000
VERSION     DAOOYB010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13334
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4737_length_13334_cov_9.283304"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            37..1335
                     /locus_tag="PL056_05220"
     CDS             37..1335
                     /locus_tag="PL056_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46449.1"
                     /translation="MKVFKIYFLSLLLIFAFPVLAQESAGEGETEPGKSVSEDAEEDV
                     EEEEDEAEDEAEAKEEKPAEAKPEEVKPAEAKPEPVAEKPAEAAPAEVKPEPVAEKPA
                     EAPQPKVAVKIESEPEDEKGKDDDKEKENKKFNISVINGFSHGFAKERKAFSYNLTLG
                     ATYMFPWQIAFSADVGLNALYRYDLDSSVATEAGYTTDENIDSGKFDGTPLNLGLSKG
                     FPVVWGIMGNLGINAGLPFTSTELWEQYEIYTMLTASFGVARPFKVGKETSVTPAFNF
                     SYTKTFAKNDYAMDEYQNDISSPINEHTFVMGLNLSLAYKGLKIAVNGGYVISRNYSV
                     TSMPDDLYGDGDYAGTKIGSGTYQDWSHVFSFGASVSYSIKEWSFGIGVMTNAPEYDS
                     GNYTYNAVPGDPDVNNGSSNYPFKPKYTRVFANIGYSYSF"
     gene            1353..6560
                     /locus_tag="PL056_05225"
     CDS             1353..6560
                     /locus_tag="PL056_05225"
                     /inference="COORDINATES: protein motif:HMM:NF027637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HPM46450.1"
                     /translation="MNWFKRAFSLLAALIVVMIAVSCTTERDAVDKTPELSLDKRLFE
                     GEFFLRATIVDIPYTVDYSFIGESNDGKVIKWKITENWLIAYSVWDKLNSIDTNEIID
                     VNETPVFAYPIAMHYDIVPSENPTTGEALPVLSPNTDRPWQERRYFTLAYDTTSGVNS
                     FEYKYLNMTMEEGAPFYKYGINIGTDWEFYAHDGTYIVPKKYKEYTAIADDKERFNKE
                     VEWFQFYSTEYLAPLNSWDSIYTMEDVEEFIGSEPAGMTQRYVFTKVNRDVVGKRDYD
                     LATRSFMWKKGSKDSGFRPLEFRDELFREYGFFTNTFRGYDNYHGYKDDNFHILANYW
                     NMAWANEKKEWNFKCGNSETYNDCLKKVKYQQKIVFVSSPKTPLRMIPINCAITKDYN
                     HAMLAARYAAMNPGSDTRQFEKWYVDNYKNDFFKTENGQTIRDQYWYLNELPEDWKTK
                     CFIPDEYAWIQRWDGSFIDENGDDTVNTDMEKILEEYKNDIVVMVENHVESYVPARDG
                     KSLYAFDNYHNYSNESRENAVKVCIDMEEAAKYREDVVMQYTTCVTNNTGMSKLYVDE
                     AGNPEWRGDRWGCKVFAQDEDCPEGTVEAKAACVLNEERSCEVEAGLPVLRYKYDNGN
                     PKAALINWVEKPTEYGILGVSQWNVNPETGQSLGGGSNVAGSVLAWATERAVEMARMV
                     ISKDDPAAWDFEDLLNPDYAKYPEVAWNNNPQTYEGKSLKKRVPQNSMKMINEPLPKF
                     NPSLPMEMQDDVLSKYNDLFMATTQKYDRFDYSSIKGTKWETKMVPYSIKRALYPWAD
                     PADNPSYSDAEREIMEPWYGGPALMEAQMLRWMDARARDFDYDESFLDGTIINFIKAK
                     QLELRGQITDWDKGGKDKYEKALIYSIYDELEKLMFKGVAQHEMGHTVGLRHNFAGST
                     DINNFGDAYFAERNYPAMMEGVETLVKDEIAKPDYNPTLLGDKVYRFKKEFDSDINYY
                     TYNSVMDYQREAYIHANGLGKYDSAAIKFVYGRSIEQYMVEGGNKYGLIVKEGDSNDP
                     NPELVNKVRDERYPDFVKVNTPFIDEKTGKLAKVEKPREVVEYYENGEKVTRMIPRFQ
                     RTLLGNMMKNDDGSLKWGHDCDGSDVDQYYCEGDTTYLVNDGSHYEYIFVSDEKSYDE
                     PKANTFDGGYLAADIIRQMVDMDDMFYYLRFFRRGNPKFREFRGRTSMKMIYDSLFKE
                     YKFVHFLLDYNFYAYGKWFRHMPLWNSEDGCSTMQIPVGETEDTFIDVEDYLCQEHSK
                     ALAGIEYWVDPTTDKVRGMTPMGAGDHVLAGMEGIQYLLFERIYKPSAGEYMKVDNDA
                     NASTKMLPGQTFYYQSTDNYIDGISSDPSPFGISKSDFMTVPASMGRYHRDRYDIQDN
                     PGIYYDKVIRRGFAEEKMVSLFTLSNSGWFDGKYRRESRANSLGQLFDGLTNVIFNVL
                     SDIATEEAVMTFSPYCSNSDISNNMVKAQYPTNLLTNFGALPYFWPQGSTLEGAYEPA
                     INICSMIDPVKPEKYVPLHAGWIYFDKMWPNYWAMGNLANISADTSVLRRWMSGVIPA
                     YAIGLYPTPDVNEEQFLNQDESVYYRAQIPVSAMDNLDKTAFRAWRECREGLIENGID
                     DFTPCLASAVWPDWYLTDIITANNPTWTVTQVNDELDRMKTDGENVRFASEVEFKKFL
                     ADKYARYSPAYRIVRDAKILKENEIEEAASGSTEPFSRLVIMETTLDQLNSFAYDQFG
                     QAAYYVSHY"
     gene            6759..8360
                     /locus_tag="PL056_05230"
     CDS             6759..8360
                     /locus_tag="PL056_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46451.1"
                     /translation="MRKFLICSAVFLYFTDLFPSPLSETVGAMGTTNPFNSRVFAQGA
                     EVTYFNPALLTLMERRFSLNFFYSYQNLDISLMDKPVGSDVIGDVDEGTGIYGATQQG
                     TAPLETNLPFKTRPTADLDERGGHSTKDGEFYGALGLVFPLWEDYLALGFYGLIPIGN
                     LMKQDSFFVDEREANFSNSLHYELYDDRMSSFNLSLALSGGYKYVFAGVGVTATAEAV
                     INSTVFTPDAGKNENKIHADTTIKTKFTPHFGLIIKPWNFLNIGFTAHLPSKTGVDTK
                     NKITFWYIDREKEAEINTNNLEVAYSYKPLSLSPSIALVDFEISKDLKLSAGFTAIWR
                     QWSKYVNRYNEKPEDNIFWDSEMEIKDKEGNIQSIGGWAYEEIDRFKWKDSWEFIIGT
                     AIEYKALKVGLDLGYYMTPVPDQKGRTNYVDNNKVNLAAGVSYAWKIKDMKLETGINF
                     QTHIMIERETVKEEGVENSVGKDSKVVDEFPDSECDPFNPDPDCETGATMPESVGFQT
                     NNPGYPGYTSSGQIFSGGLWFALYF"
     gene            8373..9545
                     /locus_tag="PL056_05235"
     CDS             8373..9545
                     /locus_tag="PL056_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46452.1"
                     /translation="MKKILVVMIAAIVFSVSCGDDKKETGDTGNTGNTGDTGDTGDTG
                     DTGDTGDTGDTGDTGNTGNTGDTGDTGNTGNTGNTGNTGNTGDSGNTGNTGDTVAECT
                     NGEKQIGTTLCGEANKGKLYQKCENEKWVDTQECILCNPGSYPNVCGHYAQKMWFTAA
                     SKVATINMAEAWTRTYFHIVQEQDEDKVFIKAKICSIKIDNSASSILKLNMLQSFADA
                     LPLLPKESFLVDNGNGTFGFTQDVSWEIRSVDPACYGDNPGGYELPEKETDACVQDWD
                     NDTIPGLKVVATGTMSGDVHIVEKSSSKFENGWFATDGQTAGGNVIWTDEQNVVKTNN
                     SLLKSGAQNSMKVTSEHFSGPANFFEQFKIPEGSDCAYVVFYSASIFNPDPINIDD"
     gene            complement(9579..12314)
                     /locus_tag="PL056_05240"
     CDS             complement(9579..12314)
                     /locus_tag="PL056_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46453.1"
                     /translation="MKKNVALLLILIFAVSCSETSTKKNKDSDIIQDFDFIELDEDAI
                     EEIDDVIFPDNSQEPDDFENIDDPDNEEQTDEDETVDETVDELNDETVDDDVFEGNSI
                     EWLGDTATVSYTGNELKTFTISTTADLRDNTPADKKRTFSQKEGDMILKSGNIMFDAL
                     FTMALREVEENSVSQISDWSFNNGNPVPCDCFETGAKWHYVWTRDTSYAVDLGLAFVD
                     PERSKNSLSFKISERKASAGGSNMEIIQDTGSGGSWPVSTDRVSWALGAYETLKYLEG
                     SSYTDFLDKAYTAIKNTVMTDRSHVFDAADGLYYGEQSFLDWREQTYPVWTASDTVHI
                     AMSKTLSTNVLHYIILDTGSKLAEIKGDAAFKNDCDTWKNSLKNSIVDKFYLSDAKLF
                     SAMKTTFLNDAAVKKFDLLGQSLAVIAGIADESKAKDAVANYPVTTAGPPVIWPQEPS
                     TRIYHNRGIWPFVTAYALRSAVKTRNDMAYENAFMSIVRGAALNLSNMENFEFTTLSN
                     YYADNRKDQLNRDLTGPVVNSQRQLWSVAAFISLVKDGIFGMNIDMDSITFDPVIPVA
                     LRKEFFNNGEGIQIKYFKLKGKNLKIAVMFPFNDENESGQYVLGSVKFNGKVHGSTFL
                     ISELDEYSDIDLILEHSAATGTITEKNCSIESNCFAPHAPVIPTSPYGIEVNSGKLAV
                     TFTGDNGVTFNMYRDGVKVAENVTSPWTDTNSADYTDRSYCYSVSAVNSSGLESHIAN
                     PVCFWGTTFERVTKIEANGFDQTPNASDHGRSHFADWGNPAAVLSASSFTPPSSGTYL
                     VQIEYGSGRPIDTGITSCFKKVEFIDESDSSVVFSGFVTMPHLGADNWDRWGDSSFLK
                     VALNNTKTYKISITDAFNMSYFEHFVPYTGGAVGGGTEVYNRVNLSTIKFLLKEK"
     gene            complement(12333..>13334)
                     /locus_tag="PL056_05245"
     CDS             complement(12333..>13334)
                     /locus_tag="PL056_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HPM46454.1"
                     /translation="VKKGDIFFNIDTTGSMDGELYNLKHTLSSIIIPQTKAKISDSAF
                     GVAQFRDEDESSMIQINPSTDILAVQQAVNLLVAEFGGDGPEAGYYSLNNLAANGTWR
                     NETIPIAIHITDAVSHERGADRASAISALNAKGIKVITVFSEGGYDAVNALIQLSELS
                     NVTGAEVPECAGAGRTTLLYTIDENGNGLDEAVVNGIDALIKYADFDVYSLADDDGDP
                     LTADTSCFIKKVEALEFLPPPGDTCAPVAELAKMNGSTYFNGFTNFVTGTSNPAVTGS
                     MLTFKVYAQNDICIKQTKQAQQFTAVIHIVDAATNAYLDSQNVTIIVPPEIPGLGES"
BASE COUNT         4072 a         2555 c         2945 g         3762 t
ORIGIN      
        1 acggtttgag aaacttattc ataaatggag gagacattga aagtatttaa gatttacttt
       61 ctctctctgc tgcttatttt cgcttttccg gttttagctc aggagagtgc cggtgaaggg
      121 gagacagagc ccggaaaatc agtttcggaa gatgctgagg aggatgtaga ggaagaagaa
      181 gatgaagcgg aggatgaagc agaggcaaag gaagaaaaac ctgctgaagc gaagccggaa
      241 gaagtaaaac cggcagaagc gaagccggaa cctgttgctg aaaaaccggc agaggcggca
      301 ccggcagaag taaagccgga acctgttgct gaaaaaccgg cagaagctcc tcaacctaag
      361 gtggctgtta agatcgaaag cgaacctgaa gatgaaaaag gtaaagatga tgataaagaa
      421 aaagagaaca aaaagttcaa catcagcgtc atcaacggtt tttcacatgg atttgcaaag
      481 gaaagaaaag ctttttccta taatcttaca ttgggcgcaa catatatgtt cccgtggcag
      541 atagcttttt ctgctgatgt aggtcttaac gctctctaca gatacgacct tgacagctct
      601 gttgcaactg aagccggata tacaactgat gaaaacatcg attcaggaaa gttcgacggc
      661 accccgctca atctgggtct ttcaaaagga tttcctgttg tctggggaat catgggaaat
      721 cttggaataa acgcaggtct tccatttaca tcaactgaac tctgggagca gtatgaaatt
      781 tatacaatgc tcactgcaag tttcggtgtg gcaagacctt ttaaagttgg aaaagagact
      841 tcggtaactc cggcatttaa tttttcatat acaaagacct ttgcaaagaa cgattatgca
      901 atggatgaat atcagaacga tatatcaagt ccaataaatg aacacacttt tgttatgggt
      961 cttaatcttt ctcttgccta caaggggctt aagatagcag tgaacggcgg atatgttatt
     1021 tcaagaaatt attctgtcac atcaatgccc gatgatctgt acggtgacgg cgactatgcc
     1081 ggcacaaaaa taggcagcgg tacttatcag gactggagcc atgttttcag tttcggagcg
     1141 tcagtttcat attctattaa ggaatggagt ttcggaattg gagtcatgac caacgctccc
     1201 gaatatgaca gcggcaatta tacatacaat gcagttccag gcgatcctga tgttaacaac
     1261 ggatcttcca attacccttt caagcctaaa tacacaagag tttttgctaa cattggctat
     1321 tcatacagtt tctaatttaa ggaggtttta ttatgaactg gtttaaaaga gcattttcac
     1381 tgcttgcagc tcttattgtt gtcatgatcg cagtatcatg tacgacagaa agagatgcag
     1441 tggacaaaac acctgaactt tctctggaca agagattgtt cgaaggagaa ttctttctcc
     1501 gtgcgactat cgttgatatt ccctacacag ttgattattc tttcataggg gaatcaaatg
     1561 acggaaaagt gatcaagtgg aaaattaccg aaaactggct tattgcctac agcgtatggg
     1621 acaagcttaa ttcaattgac accaacgaaa ttattgatgt caatgaaaca ccagtatttg
     1681 catatcctat cgctatgcat tatgacattg taccgagtga aaaccctaca acaggagagg
     1741 cacttcctgt tctttctcca aataccgaca gaccgtggca ggaaagaaga tattttactc
     1801 ttgcctatga cacaacgagc ggtgttaatt catttgaata caaatatctt aatatgacaa
     1861 tggaagaggg cgctcctttc tacaaatacg gtataaatat cggtacagac tgggaattct
     1921 atgctcatga cggaacctac attgttccaa agaaatataa agaatatacc gccatagctg
     1981 atgacaagga aagattcaac aaagaagttg agtggtttca gttctattcg acagagtatc
     2041 ttgctcctct taacagctgg gattcgattt acacaatgga agatgttgaa gagttcattg
     2101 gaagtgaacc cgcaggcatg actcaaagat atgttttcac aaaagtgaac agagatgttg
     2161 tcggaaaaag agattatgac cttgctaccc gttcatttat gtggaaaaaa ggttccaagg
     2221 atagtggttt cagacctctt gaattcagag atgaactttt ccgtgaatac gggtttttta
     2281 caaatacatt cagaggttac gacaactatc atggttacaa ggatgacaat tttcacattc
     2341 ttgcaaacta ttggaatatg gcatgggcaa atgaaaagaa agaatggaat ttcaaatgcg
     2401 gaaacagcga aacatacaat gactgcttga aaaaagtgaa atatcagcag aagatagttt
     2461 ttgtttcttc tccgaaaact ccgcttagaa tgattccgat aaactgtgca atcacaaaag
     2521 attacaacca cgcaatgctt gccgcaaggt atgccgcaat gaaccctgga agtgataccc
     2581 gtcagttcga aaaatggtat gttgacaact ataagaatga tttctttaaa acagagaatg
     2641 gacagactat tcgtgaccag tactggtatc ttaatgaact tccggaagac tggaaaacaa
     2701 aatgttttat tcctgatgaa tatgcatgga tccagagatg ggacggttca ttcattgatg
     2761 aaaatggtga cgatactgtt aatacagaca tggaaaagat acttgaagaa tataagaacg
     2821 acatcgttgt aatggtagaa aaccatgtcg aatcttatgt tcctgcaaga gacggaaagt
     2881 cactttacgc ttttgataat tatcacaact attcaaatga aagccgtgaa aatgctgtga
     2941 aagtctgtat agatatggaa gaagcggcaa aatacagaga agatgttgtt atgcagtaca
     3001 ccacctgtgt tacaaacaac accggaatgt cgaagcttta tgttgatgaa gccggcaatc
     3061 ctgagtggag aggtgaccgt tggggttgta aagttttcgc acaggatgaa gattgtcctg
     3121 aaggaacagt tgaagcaaag gcggcatgtg ttcttaacga agaaagatct tgtgaagtag
     3181 aggcaggtct tccggttctc cgttacaaat atgacaacgg taatccgaaa gcagctctta
     3241 taaactgggt tgaaaaaccg actgaatacg gtattctcgg tgtttcacag tggaatgtta
     3301 accctgaaac aggtcagtct ctgggtggcg gttccaatgt tgcaggttct gttcttgcat
     3361 gggcaacaga aagagcagtt gaaatggcaa gaatggtcat ttcaaaagat gatcctgctg
     3421 cttgggattt tgaagacctt cttaatccgg actatgcaaa ataccctgaa gttgcctgga
     3481 acaacaatcc acagacctat gaaggcaaat cgcttaaaaa gagagttcct caaaacagca
     3541 tgaaaatgat taatgagcca cttcctaaat ttaatccgag ccttcctatg gaaatgcagg
     3601 atgatgttct gtcgaaatat aacgaccttt ttatggcaac aacccagaaa tatgacagat
     3661 ttgattacag ctcgataaaa ggaacaaagt gggaaacgaa gatggttcct tattcgatta
     3721 agagagctct ttatccgtgg gctgatcctg ctgacaatcc ttcctacagc gatgcagaaa
     3781 gagagataat ggaaccgtgg tacggtggac cggctcttat ggaagcacaa atgcttagat
     3841 ggatggatgc aagagcgcgt gactttgatt atgatgaaag ttttcttgat ggaacaataa
     3901 tcaatttcat caaggcaaaa cagcttgaac ttcgtggtca aatcacagac tgggacaaag
     3961 gcggaaagga taagtatgaa aaagctctta tatactctat ctatgatgaa cttgaaaagc
     4021 ttatgtttaa aggtgttgca cagcacgaaa tggggcatac agttggtctt agacacaact
     4081 ttgcaggatc aaccgacatc aacaattttg gtgatgcata ttttgctgaa agaaattatc
     4141 ctgcaatgat ggaaggagtt gaaactcttg taaaagatga aattgcaaaa cctgattaca
     4201 acccgactct tcttggtgat aaagtttaca gattcaagaa ggaatttgac tctgacataa
     4261 attactacac gtataattca gtaatggatt accagagaga agcatacatt cacgcaaatg
     4321 gccttggaaa atatgacagt gctgcaatta aatttgttta cggccgttcg atcgaacagt
     4381 acatggtcga aggtggaaat aaatatggtc tcattgttaa ggaaggagac agcaacgatc
     4441 cgaatccgga acttgttaat aaagtcagag atgaaagata tcctgatttc gtaaaggtta
     4501 acactccttt catcgacgaa aagactggaa aacttgcaaa ggttgaaaaa ccgagagaag
     4561 ttgttgagta ttatgagaac ggtgaaaaag taacaaggat gattcccaga ttccagagga
     4621 cactccttgg aaatatgatg aaaaacgatg acggttcgct gaaatggggt cacgactgtg
     4681 acggttcaga tgttgatcag tattactgtg aaggggatac aacatatctt gtaaacgacg
     4741 gtagtcacta cgagtatatc tttgtttccg atgagaaatc ctatgatgaa ccgaaagcga
     4801 acacattcga tggcggatat cttgctgccg acataattag acagatggtt gatatggatg
     4861 atatgttcta ttacctcaga ttcttcagaa gaggtaatcc gaaattcaga gagttccgcg
     4921 gaagaacatc aatgaagatg atatatgatt cactctttaa agaatataaa ttcgttcact
     4981 tccttcttga ttataatttc tatgcttatg gaaaatggtt cagacacatg cctctctgga
     5041 actccgaaga cggttgttca acaatgcaga tcccggtagg ggaaacggaa gatacattta
     5101 ttgatgttga agactatctc tgtcaggaac actcaaaagc acttgcaggc atagaatact
     5161 gggtagatcc taccacagat aaagtaagag ggatgacacc gatgggtgcc ggtgaccatg
     5221 tccttgcggg tatggaaggt atacagtatc ttcttttcga aaggatatat aagccatctg
     5281 caggtgaata tatgaaagtt gataacgatg caaacgcttc aacaaaaatg cttccaggac
     5341 agacattcta ctatcagtca actgacaact acattgacgg tatcagttca gatccatctc
     5401 cttttggaat ttccaagagt gactttatga cagttccggc ttcaatgggc cgttaccaca
     5461 gagaccgtta tgatatccag gacaaccccg gaatttatta tgataaagtt atcagaaggg
     5521 gttttgcgga agagaaaatg gtttctcttt tcacactctc caactccggc tggtttgacg
     5581 gaaaatacag aagggaaagc agagcaaaca gtttaggaca gctttttgac ggtttgacaa
     5641 atgttatttt caatgttctt tcagacattg caacagagga agctgttatg accttctctc
     5701 cgtactgttc aaattctgac atcagcaaca atatggtaaa agcacagtat ccaaccaacc
     5761 ttcttacaaa cttcggtgca ttaccatatt tctggccgca gggatcaaca cttgaaggtg
     5821 catatgagcc ggctatcaac atctgttcaa tgatcgaccc agtgaaaccc gaaaaatatg
     5881 ttccgcttca tgccggctgg atctacttcg acaaaatgtg gccgaattac tgggcaatgg
     5941 gaaatctggc aaacatttct gcagacacat cagttctcag aagatggatg tcaggtgtaa
     6001 ttcctgctta tgctatcggt ctgtatccta cacctgatgt taatgaagaa cagttcctta
     6061 atcaggatga gagcgtttac tacagagctc agattccggt aagtgcaatg gataaccttg
     6121 ataagacagc tttcagggcc tggagagagt gcagagaagg tctgatcgag aacggaattg
     6181 atgattttac tccgtgtctt gcttcagcgg tatggccgga ttggtatctt accgatataa
     6241 tcactgccaa taatccgaca tggactgtaa ctcaggtaaa cgacgaactt gatagaatga
     6301 aaactgacgg tgaaaatgta agatttgcaa gtgaagtaga gttcaagaaa ttccttgctg
     6361 acaagtatgc aagatattca cctgcataca gaatcgtaag agatgctaaa atacttaaag
     6421 agaatgagat tgaggaagct gcatccggtt caacggagcc attcagccgc cttgttatca
     6481 tggagacaac tcttgaccag cttaacagtt ttgcatatga tcagttcggt caggccgcat
     6541 attatgtaag tcactactga tctgaaaaga aatgattttt taaagggggc ttctgccccc
     6601 ttttttttta ttcagatatt ttattattct tcaacaagtt aaaccacttg aataaaaatg
     6661 acactcgagt acagaaaaga gtgataactt cttgacagga tagagaaaat ctggtacatt
     6721 cctgtgctgt ggatgttgtt tattataagg aaaatgacat gagaaaattt ctaatctgtt
     6781 ctgctgtatt tctttatttt actgacctgt ttccatctcc tctttcagaa accgtgggtg
     6841 ctatgggaac gacaaatcct tttaattcaa gggtgtttgc ccagggtgct gaagtgacat
     6901 atttcaatcc ggcacttctt acgctgatgg agagaagatt ttcactcaat tttttctaca
     6961 gttatcagaa tcttgacatt tctctcatgg ataaaccagt cgggagtgat gtcatcggtg
     7021 atgttgacga aggaacagga atttacggcg caacccagca gggaacagca cctcttgaaa
     7081 cgaatctgcc ctttaagaca agacctaccg cagatcttga tgaaagaggc ggacattcaa
     7141 caaaagacgg ggagttttac ggtgcactcg gattggtctt tcctctgtgg gaggattatc
     7201 tggcacttgg tttttacggg cttattccga tcgggaacct gatgaaacag gattctttct
     7261 ttgttgacga aagggaagcg aatttttcaa actcactgca ctatgaactt tatgatgaca
     7321 gaatgagttc ttttaacctt tctctggcgc tttcgggcgg ttataaatat gtttttgcag
     7381 gagtaggtgt tacagcaacg gctgaagctg taataaattc tacagttttt actcccgatg
     7441 caggtaagaa cgaaaacaaa atacacgctg atacaacaat aaaaacgaag ttcactcccc
     7501 atttcggact cataataaaa ccttggaatt ttctgaacat tggatttacg gcacaccttc
     7561 cttcaaaaac cggtgttgat acaaagaaca aaataacttt ctggtatatt gacagggaaa
     7621 aagaggcgga aataaacaca aacaaccttg aagtggctta cagttataag ccattgtcgc
     7681 tgtccccgtc gatcgcactg gttgattttg agatcagtaa agatcttaaa ttgagtgccg
     7741 gttttacagc tatctggaga caatggtcaa aatatgtgaa caggtataat gaaaaaccgg
     7801 aagataatat tttctgggat tctgaaatgg agatcaaaga taaagagggc aacatccaga
     7861 gcataggtgg gtgggcgtat gaagagatag accgtttcaa atggaaggat tcatgggaat
     7921 ttataattgg aacagctatt gaatataaag ctctcaaggt gggacttgat ctaggatact
     7981 atatgactcc ggtgcctgat cagaaaggac ggacaaatta tgttgacaac aacaaagtga
     8041 atctggctgc cggagttagt tacgcatgga aaataaagga tatgaagctt gaaacgggaa
     8101 taaattttca gacacatata atgattgaaa gggaaactgt taaagaagaa ggagttgaaa
     8161 attcagtcgg aaaagacagt aaagttgttg atgagtttcc tgattcagaa tgtgatcctt
     8221 ttaatccgga tccggactgt gagacgggag cgacgatgcc tgaatctgtc ggtttccaga
     8281 caaacaatcc gggttatcct ggatacactt caagtggtca gatattttca ggcggactct
     8341 ggttcgcttt atatttctaa taggaggttg acatgaaaaa gatccttgtg gtcatgattg
     8401 cggcgattgt tttttctgtt tcatgcggcg atgataaaaa agaaaccgga gataccggca
     8461 ataccggcaa tacaggcgac acaggcgata caggcgatac tggtgatact ggtgatactg
     8521 gtgacactgg tgacactggt gacaccggca atactggaaa cactggtgat accggagata
     8581 ccggcaatac tggaaataca ggaaatacag gtaacacggg aaacacgggt gactcaggaa
     8641 acaccggtaa tacaggagat actgttgctg aatgtacaaa cggtgaaaag cagatcggta
     8701 ctacattgtg tggagaggca aataagggaa agctttatca gaaatgtgaa aatgaaaaat
     8761 gggttgatac tcaggaatgt atattatgca atccgggaag ctatccgaat gtctgcggtc
     8821 attatgcaca gaaaatgtgg tttacggctg cctcaaaagt tgccacgata aatatggccg
     8881 aagcatggac caggacatat tttcatattg ttcaggaaca ggatgaagac aaggttttta
     8941 taaaagcaaa aatatgcagc ataaaaattg ataatagtgc gagttcaata ttgaagctta
     9001 atatgcttca aagctttgcc gatgccctgc cgttgctgcc taaagaatca ttccttgttg
     9061 ataatggtaa tgggactttt ggatttacac aggatgtttc atgggaaata agatcagttg
     9121 atccggcgtg ttacggagac aatcccggtg gctatgaact gcctgaaaaa gaaaccgatg
     9181 cctgtgtgca ggactgggat aatgatacta ttccgggtct aaaagttgtc gcaacaggaa
     9241 caatgagcgg agatgtgcat attgttgaga aatcttcttc aaagtttgag aacggatggt
     9301 ttgcgactga tggacagact gccggtggaa atgttatctg gactgatgag cagaatgttg
     9361 ttaaaacaaa taattcgctt cttaaatcgg gcgctcagaa cagcatgaaa gtgacctctg
     9421 aacatttttc cggtccggca aatttttttg aacagttcaa aattcctgag ggatctgatt
     9481 gtgcttatgt tgttttttat agtgcttcaa ttttcaatcc cgaccctatc aacatcgacg
     9541 actaaccagc ttcaacttaa atcattaaca ctctgaaatt atttttcttt gagcagaaac
     9601 tttattgttg aaaggttgac acggttataa acttctgttc cgcctccgac tgctccgcca
     9661 gtgtagggga cgaagtgttc aaaatagctc atgttgaatg cgtcggttat tgaaattttg
     9721 tatgtttttg tgttgtttag cgcaactttc aggaatgacg aatcgcccca tctgtcccag
     9781 ttgtcagctc cgaggtgagg cattgtgaca aagcccgaaa aaacaactga agagtcagat
     9841 tcatcaatga attcaacctt tttaaaacac gatgtgattc ctgtgtcaat cggtcttccg
     9901 cttccgtact cgatctgaac aagataagtg ccgcttgaag gcggagtgaa tgacgaagct
     9961 gaaagcacag ctgcggggtt gccccagtca gcaaagtgag atctgccgtg gtcagatgcg
    10021 ttcggtgtct ggtcaaaacc attcgcttcg atttttgtaa ccctttcaaa tgtcgtgccc
    10081 cagaaacaaa ccggatttgc aatgtggctt tcaaggcctg acgagtttac tgctgaaaca
    10141 ctgtaacaat atgatctgtc tgtataatct gctgaattcg tatctgtcca tggacttgtc
    10201 acattttcag caactttaac gccgtcacga tacatattga aagttactcc gttatctccg
    10261 gtgaatgtta ccgcaagctt tccactgttg acttcaatgc cgtatggact tgtcggaatg
    10321 actggagcat gaggagcaaa acagtttgat tctatcgagc agttcttttc agttatcgtt
    10381 cctgttgcag cactgtgttc aagaatgaga tcaatgtcgc tgtattcatc aagttcgctg
    10441 atcagaaatg tagaaccgtg aacttttcca ttaaatttaa ctgatcccag aacatactgc
    10501 ccgctctcat tttcatcatt gaatgggaac atcacagcta ttttaagatt tttccctttc
    10561 agcttgaaat atttgatctg tatcccttca ccattattaa aaaattcttt ccttaaagca
    10621 accggaataa ccgggtcaaa ggtgatcgaa tccatatcaa tattcattcc gaaaattcca
    10681 tctttgacta aagaaataaa cgccgcaaca ctccagagct gtctttgcga attaacaaca
    10741 ggccctgtaa gatcacggtt taattggtct tttcggttgt cggcatagta atttgaaaga
    10801 gttgtgaatt caaaattctc catgtttgaa aggttaagtg ccgcacctct tacaatgctc
    10861 ataaaagcgt tttcataagc catgtcattt cttgttttta ccgcagatct cagtgcataa
    10921 gcagtcacaa aaggccatat tccacggttg tgataaattc ttgttgacgg ttcctgaggc
    10981 cagatgaccg gcggaccggc agttgtcaca ggataatttg caacggcatc tttcgccttg
    11041 ctttcatcag cgatacctgc aataacagcc agcgactgcc cgagaagatc aaatttttta
    11101 acggcggcat cgtttaaaaa tgtcgttttc attgcactga atagtttggc atcactgaga
    11161 taaaatttgt caactattga atttttaagg gaatttttcc aagtatcaca atcattttta
    11221 aaagctgcgt ctcctttgat ttcagcaagt tttgagccgg tatctaaaat tatataatgg
    11281 agcacattgg ttgaaagtgt ttttgacatt gcaatatgaa cggtgtcact tgcagtccag
    11341 acaggatagg tctgctctct ccagtcaagg aaggactgct cgccataata aagaccgtca
    11401 gcagcatcaa aaacatgtga tctgtcagtc ataacagtgt tttttattgc agtatatgcc
    11461 ttgtcaagaa agtcagtata agaacttcct tcaagatatt tcagtgtttc ataggcaccg
    11521 agtgcccacg ataccctgtc agtgctcaca ggccacgatc cgccactacc tgtatcctga
    11581 attatttcca tatttgatcc gccggcggat gcttttcttt ctgatatttt gaaactaagt
    11641 gagttctttg acctttccgg atcaacgaat gcaagcccga gatcaacggc atagcttgta
    11701 tctcttgtcc atacataatg ccattttgca ccagtctcaa aacagtcaca ggggacaggg
    11761 tttccattgt tgaaactcca gtcggagatc tgagaaacgc tgttttcctc aacttccctt
    11821 aaagccattg taaaaagtgc atcaaacatt atgtttccgc ttttaaggat catgtcacct
    11881 tctttttgcg aaaaagtcct cttcttatct gcaggagtgt tgtcacgaag gtcggctgtt
    11941 gtcgaaatag tgaatgtttt cagctcattg cccgtatatg aaacagttgc tgtgtcgccg
    12001 agccattcta tagagtttcc ttcaaaaaca tcatcatcaa cagtttcgtc atttaattca
    12061 tcaaccgttt catcaaccgt ttcatcttca tctgtttgtt cttcattatc aggatcatca
    12121 atattttcaa aatcatccgg ctcctgagaa ttgtccggaa aaataacatc gtcaatctct
    12181 tcaatcgcat cttcatcaag ttcaataaag tcgaaatcct gaataatatc agaatctttg
    12241 tttttcttgg ttgatgtttc tgaacaggaa accgcaaaaa taagaattaa cagcagagct
    12301 acattttttt tcattatttc ctcgtttgtt ttttatgatt ctccaagtcc gggtatttcg
    12361 ggcggaacga ttattgttac attctgggag tcaagatatg catttgttgc ggcatcaaca
    12421 atgtgaatta ctgctgtgaa ttgctgtgcc tgcttggtct gttttataca gatgtcgttt
    12481 tgagcataga ctttgaatgt gagcatggaa ccggtgacgg cagggtttga tgttcctgtc
    12541 acgaagttgg tgaagccgtt gaaataagtt gaaccattca tttttgcaag ttcagcaacc
    12601 ggagcacagg tgtctcccgg cggcggcaga aactcaagtg cttcaacttt cttgatgaaa
    12661 caggaagtgt cagcggtcaa aggatctccg tcatcgtcag ccagagaata cacatcaaag
    12721 tcggcatatt ttatcagcgc atcaattccg ttaacaaccg cttcatcaag tccgttgccg
    12781 ttttcatcaa tggtgtaaag aagtgtcgtt ctgcctgctc ctgcacattc agggacttca
    12841 gctcctgtca catttgaaag ttccgacagc tgaatgaggg cgtttaccgc atcatatccg
    12901 ccttcagaaa aaacagtaat gactttaata ccttttgcat taagcgcact gattgctgat
    12961 gctctgtctg ctcctctttc gtgacttacc gcatcggtta tatgaattgc tatcggaatc
    13021 gtttcatttc tccatgttcc gtttgcggca aggttgttta atgaataata gccggcttcc
    13081 gggccgtcac cgccgaattc ggcgactaaa agattgactg cctgctgaac cgcaaggata
    13141 tcagttgacg ggttgatctg gatcattgac gactcatctt catctctgaa ctgggcgaca
    13201 ccgaatgcgc tgtcggaaat ttttgccttt gtttgaggaa tgatgatgct tgaaagtgtg
    13261 tgtttcagat tataaagttc tccatccatt gatccggtag tgtcgatgtt aaagaaaata
    13321 tctccttttt taac
//
LOCUS       DAOOYB010000061        13091 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_4869_length_13091_cov_10.565357, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000061 DAOOYB010000000
VERSION     DAOOYB010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13091
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4869_length_13091_cov_10.565357"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="PL056_05250"
     CDS             <1..682
                     /locus_tag="PL056_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46455.1"
                     /translation="PVVEVQIPMEFIEYSFEPTEEVFVFTKGDIDPPVPSIADMNKTV
                     EIMQIRTKALKHSNTLERIRIKTATKSVLFGEGIESIKIFNDADKDGSFAAEELLATA
                     SISSPVSLVDVNFLPPLNYSENEEKTLLVVATFNIPKDKMAQVEILDKTSLAENVDII
                     GLPLKSKEFWYKCEEGDTSCQGEVDEKDGGCTVSAIDESSVNSILMAVLAVFGLLLFG
                     RKYGLKSK"
     gene            731..806
                     /locus_tag="PL056_05255"
     tRNA            731..806
                     /locus_tag="PL056_05255"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:764..766,aa:Met,seq:cat)
     gene            complement(912..1793)
                     /locus_tag="PL056_05260"
     CDS             complement(912..1793)
                     /locus_tag="PL056_05260"
                     /inference="COORDINATES: protein motif:HMM:NF022932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPM46456.1"
                     /translation="MSKNDELVLKLQEIGFNRLESEVYLTLLKQSPLTGYKIAQILGE
                     GSSNIYKALDILIKKGAVMVEELPSTRQFTAVSAKEYLERVEKQIAGSLKFLEENLPL
                     NGDAVNETKVFKLGNVEQVLERAVEMIENAQKQIVVDAFPKMLELIKPHLLKTARKKN
                     EKKVYVVVNSYDGTELKDCVMVKKSAGADAVKVWKDSWLNMVVDGEKYLLAVIAPDGK
                     TLRHAVWTNSPFLSYVLYSGIASEMMFSVLMDELKKREMVDIIRSALEKYSEPVMGSS
                     AQTKQLPGFQKIMKSQA"
     gene            complement(1823..2353)
                     /locus_tag="PL056_05265"
     CDS             complement(1823..2353)
                     /locus_tag="PL056_05265"
                     /inference="COORDINATES: protein motif:HMM:NF021363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HPM46457.1"
                     /translation="MTSNMINMEPPRKVPWYDLKGQFVQIWKKLLSLNDSPHDIALGL
                     ALGIFIGFLPIMGIQMAVVLVFALPFRNANKVAAVAGVWITNPLTVIPIYAFIYWVGT
                     AFYPAEYVLTSEILKCKMRDIIQLDGFIDQTQAFLSLGADIFLPMCIGGAIVGFIAMF
                     PTYFITRKLIENYRKK"
     gene            complement(2343..3653)
                     /gene="thiC"
                     /locus_tag="PL056_05270"
     CDS             complement(2343..3653)
                     /gene="thiC"
                     /locus_tag="PL056_05270"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006002019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HPM46458.1"
                     /translation="MTQLTQIESARKGIITEEVKIAAAEEKMTPEIMSRRIAEGTIVI
                     CKNRVHNVKPLAIGEGCRIKVNANIGTSEDVNSLEEEIEKAKIAVKFGADAVMDLSTG
                     KDLELTRHKIMEAAPVIIGTVPIYQAIVECVREKKKPFSDMTVDEIFEAIEKQVASGV
                     DFITVHCGVNWKLLKTIGKSKRVMGIVSRGGSITARWMAHNKQENPLFEYFDRLIDIC
                     HKYDCVFSLGDGLRPGALADATDRPQIQELITLGELTERAKDRGVQVMIEGPGHVPLH
                     QIKTNMQIEKTLCKGAPFYVLGPIVTDVAPGYDHITSAIGGAIAAMSGANFLCYVTPA
                     EHLSLPTIDDVKEGVIASRIAAHAADIANGHPGAIEWDHAMAKARRALDWEKQLELSM
                     DPEKCIKYRGDRPAEADKETCSMCGDLCAVKNFNEAEKNLENDE"
     gene            complement(3650..4240)
                     /locus_tag="PL056_05275"
     CDS             complement(3650..4240)
                     /locus_tag="PL056_05275"
                     /inference="COORDINATES: protein motif:HMM:NF014622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HPM46459.1"
                     /translation="MLWMITDFSKYRTVSEAIDHISMCLKSGVAKVTLRNTQIYSGNL
                     ICEIKDVLDARFPDKEIFIHDPSVEEIKRFRHLHFPSKRIDEAINVKKDHPEKTIALS
                     THHGTEYEKAFDNGIDFAFFSPVFKPLSKPDDIRDTVLPINVKNLYLLGGIDKMKALL
                     LIEKGFFNLAGISLFNGENAEDDIKELIKKTTEMEL"
     gene            complement(4301..4999)
                     /locus_tag="PL056_05280"
     CDS             complement(4301..4999)
                     /locus_tag="PL056_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46460.1"
                     /translation="MGLKESRTLLFLKRSSSRIFILGGIMLIVALYHDHYYSKKFFHF
                     PTKGHVVKNVSEKGVLLFSSGNKVELKNEYLSKELIKRTGSVVIIEGTTAGEFDPTLK
                     PLIRSNDYIVEINTSEVPKACKEKFVMQYPSFLRRFIYYMPQAPIFDGQKWEIDACGG
                     DLICNYTIIFHEKAGKVEMLCSGNIGDAEIAMSGDLEMNAKFNGFSEVILEISSESPE
                     LISSWKFSESSKTK"
     gene            complement(5005..6384)
                     /gene="dnaB"
                     /locus_tag="PL056_05285"
     CDS             complement(5005..6384)
                     /gene="dnaB"
                     /locus_tag="PL056_05285"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HPM46461.1"
                     /translation="MDYNPKLLPGNIEAEASLLGCLLINGKLFGTVEGTGLSSQDFYE
                     ERHSVIFKIIQSIHNEGKIADAVTVGDRISTVKGGGAGVEMKYLYELANTAPVVSESI
                     LEDYCRIIIDKSLYRELIRICSESVDSAYRQSAPFVEVVDKSTTDLIALSNRQQAGSV
                     IEIKTIVDAEMQKLKERIDSGRKISGISSGYPVLDNFGSGFKPGDMIVLAGRPGMGKT
                     SFALNMTIEIARLGHNVLFFSLEMPSSQLVQRIISIECSVNAKNLAIGKFEKDEITRL
                     WAGVDKVSKLPIFIDETSKLTVSDLRSKAKKLDAELRKSPPSTVRSNRLDCIFVDYLQ
                     LMASNVYREDKVRQVEDISRNIKLFAKELGIPIVALAQLNRKVEERKESKIPMLSDLK
                     DSGAIEQDADLVMFIHREEVYKKDTEKKNIADIFIQKNRHGQQGSVHLRFVPQYTKFA
                     TMDPSEFSK"
     gene            complement(6388..6900)
                     /gene="rplI"
                     /locus_tag="PL056_05290"
     CDS             complement(6388..6900)
                     /gene="rplI"
                     /locus_tag="PL056_05290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HPM46462.1"
                     /translation="MKIILAKDVAKLGSAGDIVKVKDGYARNYLIPKGLGLIANEKNE
                     KQLEFNKRVIEKKINTETIEAKLLASKLAACEIKIKKKVGEEGKLFGSVTNREIEAAL
                     KESGFEINHKDIVLDSNIKKSGVYEVEVKLFREVKGVFKLWVISEDSESVVSEQTPVE
                     ADTAEKSEQE"
     gene            complement(6911..7135)
                     /gene="rpsR"
                     /locus_tag="PL056_05295"
     CDS             complement(6911..7135)
                     /gene="rpsR"
                     /locus_tag="PL056_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HPM46463.1"
                     /translation="MVAYKKRKVCRFCIHTDMEIDYKNNDLLLNYITERGKIIPRRIS
                     GVCAKHQRELSREIKRARTIALLPYTVTGK"
     gene            complement(7152..7664)
                     /gene="rpsF"
                     /locus_tag="PL056_05300"
     CDS             complement(7152..7664)
                     /gene="rpsF"
                     /locus_tag="PL056_05300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HPM46464.1"
                     /translation="MERRHYELTAILKPEGSPDVIRQFAERVDGIINDFGGKTITLRS
                     WGEKKLAYEIKKNMKGHYLFFDLVGDGKMIEEVERNFNIWEHVLKFMSIKVEKKENLD
                     ELIANAKGITTLFEKAERKQVYTEVKNVEPVEEVKEKEKKVLDDSRKYNEELQKSLNV
                     SGEEEEDVEL"
     gene            7934..9931
                     /locus_tag="PL056_05305"
     CDS             7934..9931
                     /locus_tag="PL056_05305"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46465.1"
                     /translation="MNFFKLSMFSVVFLSVFFISAVTTMDGDDIGPEISVYSGSELKW
                     EEGNRDFFVMFKSLLDRKNDTYNIGSDGKLNKLNPQADSCKESATFELSDFHLPPDAY
                     VTDAFLIWNAAQPAAKLAELTDNSVHLKFTDSSGNVIIDKEITAPVHNKLDGEKSFDF
                     ESFAGIRQYPNIDYTAPEDGEMGYYTYRVNITDVFEEAHAAGRDSGISDSRNALLGKW
                     EVSGLDCATDDFPYLRGTTLVSNWSIFFVYTSSAPGVKPKKIYVYNGFKDYQDKSQDI
                     LVKGFKFPNDPSIRVTILSSEGDSANYVANSTDHKIPEGLKFRSDISLNWAPVFNICH
                     PHREPYAFAGKGYDDIFNSISSVFGWNSTTPFCVGGRIDSPGEVDIATAEWAIDVDTF
                     LLNSFDFPGYLNEGDTDIEIQLNMNNDVVYTNMMIVSLDTAPADFDIPEESEKNFCSC
                     SSKQNSICSDSSFYYTVKVENWSEKSAGGVVVTDKLPQEIEYVAGTTEIASLFDETGA
                     GINWTAIADGPGGSFPLESGYYVDDSLEPCSFESCPESYLIRFKVKPKAGLPKNAAIK
                     NTAIISDSAGVTYRTNSDVPLRLVFDAACPETAECSAPDRCSPSCGGCGTDGTDDNGN
                     GSDDDKGTSGTDDSENDSKEELNDSGNIPEDEETGCAIIVI"
     gene            10020..11213
                     /locus_tag="PL056_05310"
     CDS             10020..11213
                     /locus_tag="PL056_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HPM46466.1"
                     /translation="MYRFISFIGIAVFIAIAWLLSENRKKVDFKIVINGLLIQFAFAL
                     FILKTPPGKMIFNFLNKIFSKMIDFTNAGSEFVFGGLLKEFSFAFSILPTIIFFSSFF
                     AVLYYFGILQFFVKIFAWMLAKILKTSGAESLSAAANIFMGQTEAPLMVRPYIEKMTR
                     SELMCLMTGGLATVAGGVLAAYVNMGINAGHLIAASVMSAPAAIIIAKIMVPETEKPL
                     TSSITGIKIEVTDRNALDAAARGAGEGVYLAINVAAMLVAFIAFVALFNYVFSIFGTS
                     LEGIFGMIFKPFAFLMGVPWEETSQVGSLLGEKTVLNEFIAYDSLAKMNKDVATALSE
                     RSTVIATYALCGFANFGSIAILLGGIGTMAPSRRGELASLGIKALIGGTIACFITANI
                     AGILI"
     gene            complement(11215..12462)
                     /locus_tag="PL056_05315"
     CDS             complement(11215..12462)
                     /locus_tag="PL056_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004079659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM46467.1"
                     /translation="MEFLKIEEILSDQREVFGGKSPGIERNINFETALKHERIVVVSG
                     VRRAGKSTLLRQFAQKMDDWHYANFDDERLINFSVDDFQTMMVIFHKRSLSKNIIIDE
                     IQNVDGWERFARRIHDEGYKLFVSGSNAKMLSSELGTHLTGRYSKIELFPFSFKEFIN
                     FKGLDLQTVTTAVKAQVLNYFDEYLLTGGFPEYLNFNDTEFLKRTYDDILYRDIVSRY
                     GIRNVKQFKQLSHYLFTNFTKDTNYSTLKDNLNFTNTTTVSNYIHYLEESYLLFEVFQ
                     FDYSLKKHQSNQKKIYAIDNGLRNSIAFQFSKEYGNHLENMIFIELKRRGCDIYFYRS
                     KKECDFVIVDREKVSKVIQVTYSLNDANRERETNGLLEAMKELKTDNGIIITAHQDET
                     LRIDNKDISVISAWRWLADSQLN"
     gene            12735..>13091
                     /locus_tag="PL056_05320"
     CDS             12735..>13091
                     /locus_tag="PL056_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46468.1"
                     /translation="MLTAFFVFLLVSCGKSEEKLKNDNNLNDSDVEKYEDDSNAGDSD
                     ITGDETVSESDETVNDDDAVIIIEKDKITGFVQKGPFINGTSISLFELDENLVQTGKI
                     YNTQITKNDGIFELADV"
BASE COUNT         3641 a         2838 c         2432 g         4180 t
ORIGIN      
        1 tcctgttgtt gaagttcaaa tcccgatgga attcattgaa tactcttttg agcccaccga
       61 agaagttttc gtatttacaa aaggtgatat agatccccct gtgccgtcaa ttgctgacat
      121 gaacaagacg gtcgagatca tgcagataag gacaaaagca ctcaagcaca gcaacactct
      181 tgaaagaata aggataaaaa ccgctactaa atcagtcctt ttcggtgaag ggatagaaag
      241 catcaaaata ttcaacgatg cggacaaaga cggatcattc gcggctgaag aattgcttgc
      301 aacagcttcg atatcctctc ctgtgagtct tgttgatgtt aacttcctgc ctcctttaaa
      361 ctacagcgag aatgaagaaa agacccttct tgttgttgca actttcaaca ttcccaaaga
      421 caaaatggca caggttgaga ttctcgacaa gacctctctt gctgaaaatg ttgacatcat
      481 cgggcttcct cttaaatcaa aagagttctg gtacaaatgt gaggaaggtg acacaagctg
      541 tcagggtgaa gttgatgaga aagatggcgg ctgtacagtt tcagcaattg atgaatcatc
      601 agtaaattca atactcatgg cagttcttgc tgttttcgga cttctccttt ttggaaggaa
      661 atacggatta aaatcaaaat aaaggttgac aactgaatct tagtaactac tatatcacct
      721 gatctaaggt tgcggggtag agcagctggt agctcgtcgg gctcataacc cgaaggtcat
      781 tggttcaaat ccaatccccg cgaccatttt tctttttatc tccctcacag atcaagctaa
      841 acggataaca cattgtggtt attagtcttt tgccatcaag cactatcttg tgaaacatca
      901 gttccagcag tttacgcctg agatttcatt attttctgga atccggggag ctgttttgtc
      961 tgggctgaag accccattac cggctcagaa tatttttcaa gagctgatct tatgatgtca
     1021 accatctctc ttttcttaag ttcatccatc agaacagaga acatcatttc agaagctatc
     1081 ccgctgtaga gaacatagct taaaaacggg ctgttagtcc acactgcatg tctaagtgtc
     1141 tttccgtcag gtgcaatgac cgccagaaga tatttttctc cgtcaaccac catgttaagc
     1201 cagctgtctt tccacacttt tacagcatca gctcccgcgc tcttcttaac cattacacaa
     1261 tctttaagtt ctgtaccgtc atagctgttg accacgacat aaaccttttt ttcatttttc
     1321 tttctagccg ttttcagtaa atggggtttt atcagttcaa gcatcttcgg gaaagcatca
     1381 acaacgatct gcttctgagc gttctctatc atttcaacag ccctttcaag cacctgctcg
     1441 acatttccca gtttaaaaac ttttgtttca ttgaccgcat caccgtttaa cggcagattt
     1501 tcttcaagga atttcagact gcctgctatc tgcttttcga ccctttcaag atattctttc
     1561 gctgacacag ctgtgaactg cctcgttgaa ggaagctcct caaccattac agctcccttc
     1621 tttatcagaa tatccaatgc cttatagata tttgaagacc cttctccaag tatctgggca
     1681 atcttatatc ccgtaagagg cgactgtttt aaaagtgtaa gataaacctc ggattcaagg
     1741 cggttgaacc ctatctcctg aagttttaaa acaagttcat cattttttga catttccccc
     1801 tcccctctct aacacacaca acttatttct ttctataatt ttcgatcaac tttcttgtaa
     1861 tgaaatatgt cgggaacatt gcgatgaatc cgactatcgc cccgccgatg cacatcggca
     1921 gaaatatatc agcgcccaag ctgaggaacg cctgcgtctg atcaatgaat ccgtcaagct
     1981 ggattatatc tctcattttg cacttcagaa tttcagatgt caggacatat tccgcgggat
     2041 aaaaagcagt tcccacccag taaatgaatg catatatcgg aattacagtc agcgggtttg
     2101 ttatccacac gcctgctact gccgcgactt tgttggcatt tctgaaagga agtgcaaaaa
     2161 caagaacaac cgccatctga atgcccatta tcggaagaaa accaatgaaa attccaaggg
     2221 caagacccag tgcaatgtca tgaggagagt cgttaagaga aagtagtttt ttccatatct
     2281 ggacaaactg acctttcaga tcataccacg gaacttttct gggcggttcc atattgatca
     2341 tgttactcgt cattttctaa attcttctca gcttcgttaa aattctttac agcacagaga
     2401 tctccgcaca tcgagcaggt ttccttgtct gcttcagccg gtctgtctcc ccggtatttg
     2461 atacattttt caggatccat tgaaagttca agctgttttt cccagtcaag agcacgcctt
     2521 gcttttgcca ttgcgtgatc ccattcgata gctccgggat gaccgtttgc aatatctgcc
     2581 gcgtgagccg caattcttga agcgatgact ccttccttca catcatcaat tgtcggcaga
     2641 gacagatgct cggcaggtgt gacataacag agaaagttag ctccgctcat tgccgctata
     2701 gctccgccga tagctgaagt aatgtgatca taacccggag caacatcagt cacaatagga
     2761 ccgagaacat agaacggcgc acctttacac agtgtttttt ctatctgcat atttgttttt
     2821 atctgatgca gcggaacatg ccctggacct tcgatcatga cttgcactcc tctgtccttt
     2881 gccctttctg taagttctcc aagagttata agttcctgaa tctgaggacg gtctgtagcg
     2941 tcggcaagag caccgggacg gagtccgtcg ccaagagaga acacacagtc atacttgtga
     3001 catatatcaa taagccggtc aaaatattca aaaaggggat tttcctgttt gttgtgggcc
     3061 atccaccttg cagtaatact tccgcctctt gagactattc ccatgaccct ttttgatttt
     3121 cctattgttt tcaacaactt ccagttgaca ccacagtgaa ccgttataaa atctactccg
     3181 cttgcgacct gtttttctat cgcttcgaat atttcatcga cagtcatatc ggaaaatggc
     3241 tttttctttt ccctgacgca ttcaacaatc gcctgatata tcggaacagt tccgataatt
     3301 acaggagccg cttccattat tttatgtcgt gtcaactcaa ggtccttgcc tgttgaaagg
     3361 tccatcaccg catcggcacc gaacttaacc gctatctttg ccttttcaat ctcttcttca
     3421 agtgaattaa catcttcgga agtgcctatg tttgcgttta cttttattct gcacccctca
     3481 cctattgcaa gcggcttgac attgtgaacc ctgttcttgc atattacaat tgttccttca
     3541 gcgatccttc tgctcataat ctccggcgtc attttctctt cggcggcggc gatcttaacc
     3601 tcttccgtta ttattccctt tcttgctgat tcgatctgag taagttgagt cataattcca
     3661 tctccgttgt tttttttatc agctccttaa tatcatcttc tgcattctct ccattaaaaa
     3721 gcgagatgcc cgcaagattg aaaaatccct tttcaataag caaaagggct ttcattttat
     3781 ctattccgcc aagaagataa aggtttttca catttatcgg caaaacagta tcccttatat
     3841 catcaggttt ggataagggt ttgaaaacag gcgaaaaaaa tgcaaaatct attccgttat
     3901 caaatgcttt ttcatattca gttccatgat gagttgaaag agcgatggtc ttttcaggat
     3961 gatctttttt gacatttatt gcctcatcta tcctttttga agggaagtgg agatgcctga
     4021 atctttttat ctcttcaact gacggatcgt gaatgaatat ctctttatca gggaaccttg
     4081 cgtcaagaac atcctttatc tcacaaatta aatttcctga ataaatttga gtatttctaa
     4141 gagttacttt cgccacacca gattttagac acatcgaaat atgatcgatc gcctcactta
     4201 ccgttctata ctttgaaaaa tctgttatca tccagagcat tgttcacctc cggatgtaaa
     4261 ttaaccccta aaaacaagtt tgtcaaacag ggatggagat ctattttgtt tttgaacttt
     4321 ctgagaattt ccaacttgag atcagttcag gagattctga ggatatttcg agaattactt
     4381 ctgaaaatcc gttgaatttt gcattcatct caagatctcc tgacatcgca atttccgcgt
     4441 cgccgatatt gccggaacac agcatttcca cttttcctgc tttttcatga aatattattg
     4501 tataattaca tatcagatct ccgccacaag catcgatctc ccatttctgt ccgtcaaaaa
     4561 ttggagcttg aggcatatag tatatgaatc tccttaaaaa cgaaggatac tgcatcacaa
     4621 atttttcctt acacgctttc ggcacttccg atgtatttat ctccacaata taatcattcg
     4681 atcttattaa cggtttcagc gtcggatcaa attctccggc agtggtgcct tctatgatca
     4741 cgactgaacc ggttcttttt ataagttctt ttgaaagata ttcgttcttc agctcgactt
     4801 tatttccgga tgaaaacaaa agaactccct tttcgctaac attctttacc acatgtcctt
     4861 ttgtcggaaa atgaaaaaac ttttttgaat aatagtgatc atgataaagt gccacgataa
     4921 gcattattcc gccgagaatg aatatccttg agctcgatct ctttaagaaa agaagggttc
     4981 tgctctcttt taaccccatg tgaactactt actgaattct gacgggtcca ttgttgcaaa
     5041 ttttgtgtat tgaggtacaa aacgcaaatg gaccgaaccc tgctgtccat gacggttttt
     5101 ctgtatgaat atatctgcaa tatttttctt ttcggtatct ttcttataaa cttcctctct
     5161 gtgaatgaac attacaaggt cggcatcctg ttcaatggct cccgaatcct taagatcact
     5221 cagcataggt attttacttt ccttcctctc ttcaaccttt ctgttgagct gtgcaagggc
     5281 gactatcgga ataccgagtt cctttgcaaa aagcttgatg ttccttgaaa tatcctccac
     5341 ctgtctcacc ttgtcctctc tgtagacatt tgaggccatt agctgaagat agtcaacaaa
     5401 tatacagtca agacggttgg accttacagt ggaaggcgga gactttctaa gctccgcgtc
     5461 aagtttcttt gctttagatc taaggtctga aacagtgagc ttggatgttt catcaataaa
     5521 tatcggcagt ttactaactt tatcgacacc tgcccaaaga cgggtgatct catctttttc
     5581 aaattttccg attgcaaggt tttttgcatt tacactgcat tcaattgaaa ttatcctctg
     5641 cacaagctga cttgacggca tttcaagtga aaagaaaagg acattgtgac caagtctcgc
     5701 tatctcgatg gtcatgttca gagcaaaact tgtcttaccc atgccgggtc ttcccgcaag
     5761 aactatcata tcaccgggtt taaaaccgct gccaaaatta tcgagaacag gataaccaga
     5821 tgaaatacct gatattttcc tgccgctgtc aattctctcc ttaagtttct gcatctctgc
     5881 atcaacaatg gtttttattt caatgacact tcccgcctgc tgtctgttgc tcaaagctat
     5941 cagatcagtg gtggatttat cgacaacctc aacaaaaggt gcagactgtc tgtatgcact
     6001 gtcaacgctt tcagaacaaa tacggatgag ctctctgtag agtgatttat caatgattat
     6061 tctgcagtaa tcttcaagta tcgattcaga aacgaccgga gcagtatttg ccagttcata
     6121 gagatatttc atctcgaccc cggcacctcc gcctttgaca gtacttattc tgtcaccgac
     6181 agttaccgca tccgcaattt taccttcatt atgtattgac tggatgatct tgaatatcac
     6241 agaatgtctc tcttcataaa aatcctgtga agaaagtcct gttccttcaa ctgttccgaa
     6301 aagttttcca ttaatgagga gacatcccag aagagatgcc tccgcctcaa tgtttccggg
     6361 cagcagtttt ggattataat ccattaacta ctcctgttct gatttttccg ctgtatctgc
     6421 ttctaccggt gtctgttctg aaacgactga ttcactatct tctgatatta cccagagttt
     6481 gaaaacacct tttacttctc tgaaaagttt tacttcaact tcataaacac cgcttttctt
     6541 gatattgctg tcaagaacga tgtccttgtg attgatctca aaaccgctct ctttgagtgc
     6601 cgcttcgatc tctctgttgg tgacactgcc gaaaagcttc ccttcctcac ccactttttt
     6661 ctttatcttt atttcgcatg cggcaagttt tgatgcaaga agttttgctt cgatagtttc
     6721 ggtgtttatc ttcttttcga taactctctt gttgaattca agctgttttt cgttcttttc
     6781 attggcaata agtccgagtc ctttcgggat aagatagttg cgggcataac cgtccttaac
     6841 ttttactata tcccctgcac ttccgagttt tgccacatct tttgcaagta ttattttcat
     6901 ttacgccccc ttatttgccg gttactgtgt atggaagaag agcgatcgtt cttgctcttt
     6961 ttatctccct tgaaagctct ctctgatgtt ttgcacagac accgctgatc cttctgggta
     7021 ttatttttcc tctttcagtt atgtaattaa gaagaaggtc attattttta taatcgatct
     7081 ccatatcagt gtgaatgcag aaacggcata cttttctctt tttatatgct accattgttt
     7141 tctcctctca tttaaagttc aacatcttct tcttcttcac ctgaaacatt cagggatttc
     7201 tgaagctctt cgttatactt ccttgaatcg tcaagcactt tcttttcctt ttccttgacc
     7261 tcttcaacag gttcaacatt tttcacttcc gtataaacct gtttccgttc agctttttca
     7321 aaaagtgttg tgatcccttt tgcgtttgcg ataagttcat caaggttttc tttcttctct
     7381 accttaatgg acatgaattt aagtacatgt tcccagatgt tgaagtttct ttccacttct
     7441 tcgatcattt ttccgtcacc caccagatca aagaaaaggt aatgaccttt catgttcttt
     7501 ttgatctcat aagcaagctt cttttctccc catgaacgga gagtgatggt ttttccaccg
     7561 aaatcattga tgattccgtc aacgcgctct gcaaactgcc tgatcacatc agggctgcct
     7621 tccggcttta agatggcagt aagctcataa tgtcttcttt ccataattcc tccttacggc
     7681 tgtgaagtcc aaacataaaa ttgttgaaca aggagatgtt cccagtgttc aaaaaacaaa
     7741 aagcttatgg attttagtta taaaagccgg acttgcaact tttttcttta tttaaaaaat
     7801 gtatttcagt ccgtctttct aattccagac tgcaattaac agaaaaaaag tcctgacttc
     7861 acatccggac gaatattatt ttaaaaattc atatttttca gagttgtttt tattgaaaaa
     7921 gcaggaggaa ttcatgaatt ttttcaaact gtcaatgttt tcagttgttt ttttaagtgt
     7981 tttttttatt tccgctgtca caaccatgga tggagatgat atcggacctg aaatttcggt
     8041 ctacagcggt tctgagttga aatgggagga aggcaacagg gatttttttg tgatgttcaa
     8101 gtcacttctt gaccgtaaga atgatactta caacatcgga agcgacggaa agctcaataa
     8161 attaaaccct caagctgatt cgtgcaaaga atctgcaact tttgaacttt cagatttcca
     8221 tttaccgcct gatgcttatg taactgacgc ttttctgatc tggaatgccg cacagcctgc
     8281 tgcaaaactt gctgaactga ctgataattc cgtccatctt aaattcaccg acagttcagg
     8341 gaatgtaata attgataaag agataacagc acctgttcac aacaagctcg acggtgaaaa
     8401 gagcttcgat tttgaaagct ttgcaggaat aagacagtat ccaaatattg attataccgc
     8461 tcccgaagac ggagagatgg gatattacac ctatagagtt aatattactg atgtttttga
     8521 agaagctcac gccgcaggca gagattccgg tattagtgat tcacgaaacg ccctacttgg
     8581 aaaatgggaa gtttcagggc tcgattgtgc aactgatgat tttccttatc tccgtggaac
     8641 gactctggta agcaactggt cgatattttt tgtctatact tcttcagccc ccggagtaaa
     8701 gcccaaaaag atctatgttt acaacgggtt caaagattat caggataaat ctcaggatat
     8761 acttgtgaag ggattcaaat ttccaaacga cccgtcaata agggtgacga ttctttcatc
     8821 agagggagat tcagcaaatt atgtagcaaa ttcaactgat cacaaaattc cagaggggct
     8881 gaagttcaga agcgatatat cactcaactg ggctccggta ttcaatatct gtcatcctca
     8941 cagagaaccc tacgcttttg caggaaaagg atatgacgat attttcaact ccatttcaag
     9001 tgtcttcggc tggaattcca caactccgtt ctgtgttggc ggaaggatag acagccccgg
     9061 tgaagttgac attgcaactg cggaatgggc aattgatgtt gacactttcc tgttgaattc
     9121 atttgacttt ccgggttatc tcaacgaagg agatactgat attgagatcc agcttaacat
     9181 gaataacgat gtcgtttata ccaatatgat gatagtcagc cttgacacag ctcctgccga
     9241 ttttgacatt cctgaagagt ctgaaaagaa tttctgcagc tgttcatcaa agcagaattc
     9301 catctgttca gacagttcgt tctattacac agtaaaagtg gagaactgga gtgaaaaaag
     9361 tgcaggcgga gttgtcgtta cagacaaact tcctcaggaa attgaatatg ttgcaggaac
     9421 aacagaaatt gcatcacttt ttgatgaaac cggtgcaggg ataaactgga cagcgatcgc
     9481 tgacggacca ggcggatcat ttccccttga atctggatat tatgtcgatg attcactcga
     9541 accatgcagt tttgaaagtt gtcctgaaag ctatttaata aggtttaaag tcaaaccgaa
     9601 agcagggctg ccaaaaaatg cggcaattaa aaacaccgcg ataatatctg acagtgccgg
     9661 agtcacatac agaacgaact cagatgtacc tcttcgactt gtatttgatg cggcatgccc
     9721 tgaaaccgct gagtgcagtg ctcctgacag atgcagtcct tcatgcggag gatgcggaac
     9781 agacggaaca gatgacaacg gcaacggatc agatgatgac aaaggcactt caggaacaga
     9841 tgacagtgaa aacgattcaa aagaagaact taacgacagc ggaaacatac ctgaagatga
     9901 agaaaccggc tgcgcgatca ttgtgattta gaaagaaatt gtttcataaa agtttttttt
     9961 actctttctg cttaattaat tataatcctt atcgttgttc acttatctgg agaatccgaa
    10021 tgtacagatt cataagtttc atcggtattg cagttttcat tgccattgcg tggttgctaa
    10081 gtgaaaacag aaaaaaggtt gattttaaga tcgtgataaa cggtcttctc attcagtttg
    10141 cattcgccct tttcattttg aaaactcctc cggggaagat gatatttaat tttctcaaca
    10201 agatattctc aaagatgata gatttcacaa atgccggttc tgaatttgtc ttcggcggat
    10261 tactgaaaga gttctctttt gcattctcaa ttctgccgac aataatcttt ttctcctctt
    10321 ttttcgctgt gttatactat ttcgggatat tacagttctt tgtgaagata ttcgcatgga
    10381 tgcttgcaaa aatactgaaa acaagcggag cggaaagtct ttcggcagcg gcaaatattt
    10441 tcatgggtca gaccgaagca cctctgatgg tcaggccata cattgaaaag atgacccgaa
    10501 gcgagctgat gtgcctgatg acaggaggac ttgcaacagt tgcaggggga gttcttgccg
    10561 catatgtcaa catgggaatt aacgcaggac acctcatagc cgcaagtgta atgagtgctc
    10621 cggcagccat aataattgca aaaataatgg tccccgaaac tgaaaaacct cttacatcaa
    10681 gcataacagg aataaagatc gaagtgaccg accggaatgc tcttgatgcc gccgcccgtg
    10741 gtgcaggtga aggagtatat cttgccataa atgttgccgc aatgcttgtc gccttcatcg
    10801 cttttgtcgc ccttttcaac tatgtattca gcatattcgg cacatccctg gaagggattt
    10861 ttggaatgat atttaagccc tttgcgtttc tgatgggcgt tccgtgggag gaaacttctc
    10921 aagtcggttc ccttcttggc gaaaaaaccg ttcttaacga attcattgcc tacgattcgc
    10981 tcgcaaaaat gaataaggat gtcgcaaccg cactttcaga aagaagcacc gtcattgcaa
    11041 cttatgccct ctgcggattt gcgaatttcg gttcgatcgc cattttactt ggcggcatcg
    11101 gcacaatggc cccatcaaga agaggagagc ttgcatcact tggaataaaa gcactcatcg
    11161 gcggaaccat cgcctgtttc atcactgcaa atatagcagg aatacttatc tgatttaatt
    11221 caactgacta tcggcaagcc atcgccatgc agaaataaca gaaatgtctt tgttatcaat
    11281 tctgagggtt tcatcctgat gggctgtgat tattattccg ttatcagtct ttagttcttt
    11341 catcgcttca agaagcccgt tcgtttccct ttctctattt gcatcgttta gagaatatgt
    11401 cacctgaata acttttgaaa ctttctctct atcaactata acaaaatcac actctttttt
    11461 tgagcggtag aaatatatgt cgcaacctct tcttttgagt tctataaaaa tcatgttttc
    11521 cagatgattt ccatattctt ttgaaaactg gaaagctatt gaatttctaa gtccgttatc
    11581 aatagcataa atctttttct gattagactg gtgttttttt aagctgtaat caaactggaa
    11641 aacttcaaaa agaagataag attcttcaag atagtgaata tagtttgaaa cagttgtggt
    11701 gtttgtaaaa ttgagattat ctttcaaagt actgtaattt gtatcttttg taaaattagt
    11761 gaacagatag tgcgaaagct gtttgaactg tttaacattt ctgattccgt atctgctgac
    11821 gatgtcgcga tatagaatat catcataagt tcttttcaga aactcagtat cattaaaatt
    11881 gagatactcg ggaaatccac cggtcagaag atattcatca aaataattaa gcacttgagc
    11941 ttttacggcc gtggtaacag tttgaagatc gagtccttta aagtttataa actctttgaa
    12001 agaaaacggg aaaagctcaa tttttgaata tcttccggtg aggtgtgtgc cgagttcaga
    12061 actgagcatt ttggcatttg aaccgcttac aaaaagttta tatccttcat cgtgtattct
    12121 tcttgcaaac ctttcccacc catcaacatt ctgaatttca tcaataatta tgttctttga
    12181 aagagatctt ttgtgaaata tcaccatcat cgtctgaaaa tcatcaacgc tgaaatttat
    12241 caatcgctca tcatcaaaat ttgcatagtg ccagtcatcc attttttgcg caaactgcct
    12301 taaaagagta gattttccgg ctcttctcac ccctgacacc acaacaatcc tttcatgctt
    12361 caaagcagtt tcaaaattta tgttccgctc tattccgggc gatttgccgc caaacacttc
    12421 tctttgatca ctcaagatct cttcaatctt taaaaactcc attttcacct ccactcttta
    12481 cactatacct tatcatattt cgcaaatatg tcaattgcta attgagcttt tcgcagaatt
    12541 tggagaagta tagtgcgtaa aatcaaattt ttgttttcat ctttttttct aacaatcagt
    12601 gaaatcagca tttaaattta acaataactc gaccggaatg taaactgttt ttcgctaata
    12661 ttttaaagtg agttgtgact tttggtttaa tttttttgtt gaggtttgac atgtgtttta
    12721 acagattatt tgtgttgtta acagcttttt ttgttttttt attggtttca tgtggtaaaa
    12781 gtgaagaaaa actcaaaaac gacaataact taaatgattc agatgttgaa aaatatgagg
    12841 atgacagcaa cgctggtgat tctgatatca ctggagacga aacggtttct gaatcagatg
    12901 aaactgtaaa tgatgatgac gctgtgataa ttatagaaaa agataaaatc acaggttttg
    12961 ttcagaaggg acctttcata aatggaactt ctatttcact ttttgagctt gatgaaaacc
    13021 ttgttcagac tggaaagatt tataatacac aaataactaa aaacgatggc atttttgaat
    13081 tagcggatgt c
//
LOCUS       DAOOYB010000062        13087 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_4872_length_13087_cov_10.370856, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000062 DAOOYB010000000
VERSION     DAOOYB010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13087
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4872_length_13087_cov_10.370856"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(262..1416)
                     /locus_tag="PL056_05325"
     CDS             complement(262..1416)
                     /locus_tag="PL056_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009146903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HPM46469.1"
                     /translation="MKEESKLTTNIRTFNDLVLNIVSTDEYLTCSVSKAVNKSLTLRN
                     WLIGFYIDVFELNGEDRANYGDNLFLDLSNELKEKGVSNSGKRQLYQYVKFYKTYPGI
                     VRTLSAQFKNLPENNDLEKMRTLSTQSSNTYNLNVFEMLSYSHFDELCNIDDDMKRMF
                     YEFEAVTGNWSVRELKRQIGSLYYERSGLSKDKHKLSEYVKSIAETNSSKLTVRDPFV
                     FEFLGLKSKEVMYESNLEDAILDNLQNFLLELGHGFCFEARQKRILIGGEHYFVDLVF
                     YNRILKCHVLIELKVEKFSQEHLGQLNTYVNWYRKNEMQDGDNPPVGILLCTKKNHSL
                     VEYALAGMNNQLFVSKYLTNLPDPKEIEKFLNEKMETLTEEEIKIVEGGE"
     gene            complement(1413..4844)
                     /locus_tag="PL056_05330"
     CDS             complement(1413..4844)
                     /locus_tag="PL056_05330"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TaqI-like C-terminal specificity
                     domain-containing protein"
                     /protein_id="HPM46470.1"
                     /translation="MKLSSFKTGLVEGIKNLFNDLNIPVNPITELKAEPKDILSTANL
                     ESESLKLIEAVYPYGLVNDEAFNDQKTIKISDISEKYEAIAVFGVKLGKRENGLLPTR
                     KIFADITRAFNREFKYTPVIIVFKYQDFITIASSERSEYIQKWKEGEKIGKITLLRDI
                     NIENTHTGHLKILEGLKIPTSGKNTVNSFASLHKHWQEVFDVSILNKKFYKELSNWYF
                     WAMDNVEFPDDGEKRKDVRNATNLIRLITRIIFIWFIKEKNLVPEKLFNKNEVNDIID
                     DFNKNKKSGNYYRAILQNLFFGTLNQKMGERKFAKDGGHEDKETNREEYGVKTLWRYG
                     SLFKISKLEAEKLFKDIPFLNGGLFDCLDKENEKNRTIYVDGFSRNPGKQAFVPDYLF
                     FGEETEVDLNEIYGTKNKTYSAEGLINILNSYKFTIAENTPTEEDVALDPELLGKVFE
                     NLLASYNPETKTTARKQTGSFYTPREIVNYMVDESLIAYLKTKLDDETEETDHLLHRL
                     FKHNDDQPFEDKDKVLKIVRAFDEIKILDPAVGSGAFPMGVLNKVVHVLSKLNPDNSE
                     WEELQLKKIDRKVSDTTDKTEIKELEKLRMDIMKAFCDNEADYPRKLYLIENCIYGVD
                     IQPIAIQICKLRFFISLIVDQKVDRRKDNHGIRPLPNLETKFVSANTLIGLDKPNQGG
                     IEEIFIEPLKKLLLKVRHEHFSAKTRNEKLACIKKDKEIRKQIADVLIQNHYKATDAN
                     KIADFDLYDQNKSSDWFDPEWMFGIKEGFDVVIGNPPYILLQNIQGISNQTIDKYKKF
                     VSAQYKTDLYHLFIEKGILLLSKRGSISFITPNTFIKNKYNNKLREFICVNSSILSIV
                     LFYSQVFENVSVDNLIFVFSKNKKGSLSRIYEIKKSINDISVPIRFFDSKKIIKPEYI
                     FNFDLDEKKERFLKKIHLDTKPLSFYGRSYFGIQTHDRETYVSEMKIDNNWMPVIDGG
                     NVFRYNLKKNTEFVCYIKDAIKSGGNDEVYKKNRIVIRQVGKFPEGTICSPGIFTLNT
                     IYNIFLNDNCEIDLKFLLALINSKVLRCYWILTNYDNKETFPKIKKDSIESIPIKKTN
                     INEQKRFTKIVDIIMNKKKLGQDTTDLEKQIDAMVYELYGLTEEEIRIVEGGDKQ"
     gene            complement(4971..6419)
                     /locus_tag="PL056_05335"
     CDS             complement(4971..6419)
                     /locus_tag="PL056_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007223406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM46471.1"
                     /translation="MNRYLKIIEDYFRSPKENEWIEFKENNCNPQLIGEYLSALSNSA
                     ALHGKNKAFLIFGIENGTGKIVGTNFLPSNTKGKGNEDLEPWLSRKLSPRIDFKVIEF
                     DQDEKHIVVFEIDATLNTPVKFDGEAFIRVGGHKHKLSDHPEKERKIWKKAESVSFEK
                     AVAASNLSLADVLNKIDYHNFFKLQNIPVPENQNRIIEELVQDGIVIQDDNSYSITNL
                     GALLIAKNLHDFEHLSRKAVRVIVYNGNSRVNAKNEITGKKGYAAGFKGLIDWINDLI
                     PSNEFIEEAFRVGKKRYPKLAIREFVANALIHQDFTETGRSPMIEIFDTRIEITNPGS
                     PLIDTERFIDYPPKTRNEKLASLMRRMNICEERGSGVDRALAQVEIFQLPPPLFQAED
                     DYTKVILYSYREYKDMDRIERIRACYQHACLKWVCGEYMTNSSIRERMKIDLKNSAII
                     SRIIKEALIENTIKIHDDSVGTRARKYLPWWA"
     gene            complement(6432..9785)
                     /locus_tag="PL056_05340"
     CDS             complement(6432..9785)
                     /locus_tag="PL056_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006423989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HPM46472.1"
                     /translation="MLLDNSNDNKKVYEWIEQYTENGFFDCVTGYFTIGALNHISKVI
                     NEKVKDMRFILGDIVSTGGIEDRAIDLLNENISVEAALKLSKISKEAVEFLKQEKVEL
                     KTLEPNFCHAKLYLFKSEKDDRHDYFISGSSNLTESGIGLKITGNVELNIAETGQGNQ
                     FKELKIWFNDIWKSKKAHSKKTIIHADGTKTEKDFKQYIIEEIEKVFREYLPKEIYYK
                     ILFELFWKDIGDEVESPDFNKKIGRVENTVLYNSLFDFQKSAVISLVKMIQKYNGAIL
                     ADAVGLGKTWTALAVMKFYEMEGYEVILFCPKKLDQNWRKFLHKHNSRFEQDRLNYTI
                     RYHTDLQDDRLQRYQDGLTIEDYFQSDRPKLFVIDESHNLRNERSSRYQFLIDYLLKK
                     NKTVKVLMLSATPINNSLNDIRNQFRLIVKGGDTGFTENLGIRNLMHLFRNAQREFNK
                     WSEEGTGRISDFIKKLNPDFFKLIDSLLVSRTRKHVESINEKMLNFPEKELPENIFIT
                     PKEIGNIESFKELLDHFPYMSGYQPAWYVEEKEVENVIEDEKQRDRFLVKMMYILLIK
                     RLESSWFSFKSTVEKISEHHQEVLNTIKQYNDKVKSEDIDIYGQMTIFDEEEDAEFEE
                     FTLGKKRTVNISEIDRAGKLDLLKKDLKKDIDKLDSLITNVRRFSEIVEKETGKDSVD
                     SKLKKLIQLILKKQENSNNKKVLIFTVFKDTATYLYNELTKRGFQKVAMVSGDETKSS
                     DGYNGKFFEPVLERFAPFTKLFMEKEWVGFERDNKKTDVQNFNLWIEWTKENKPEVYK
                     KIDNSVDILIATDCLSEGQNLQDCDYIVNYDIHWNPVRIIQRMGRIDRIGSPNKKIKG
                     VNFWPSESIESYLDLQKRVADKMAAMTLAGSEVPAFSKEHEEMLKDETLEQRQNAKMM
                     KLMESSWDEVQKNSEGLGFDDFSLESFRQELFSYLEDEKEKYIKMPNGVYSGFIKDGE
                     FCKKNGLVALLGSPSRPSGDKKFKYERHELVYIDENGNSLLLNQKDILRALSENKEKQ
                     RHVDKEIDNGNGEKIKKLSMAIRSWVESQAYEVDENGEKVLGKKGTDILEKIKSGNCE
                     AIKDVKGKKAIEQRYCFENYDLIAWMVIE"
     gene            complement(9942..10820)
                     /locus_tag="PL056_05345"
     CDS             complement(9942..10820)
                     /locus_tag="PL056_05345"
                     /inference="COORDINATES: protein motif:HMM:NF018009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HPM46473.1"
                     /translation="MSESLSTFGIKGMSPTNLRKFREFYQNYKIQQALPAELDKFPET
                     MQNTIEYLSSRFVLGWTHYVTLMTVKNDEERRFYEIEAAKNGWGYRELERQINSALFE
                     RLVLSRDKNGVKELSIKGQIVEKPADIIKNPYILEFTGLEPQKKYTEHELETAIIDKL
                     ELFILELGKGFLFESRQKRFSFDNRHFYVDLVFYNRLLKCFVVIDLKIGDLKHQDLGQ
                     MQMYVNYFDRYVKTEEENPTVGILLCLTKSDSLVELTLPKDRNIYASNYQLYLPSKEE
                     LKKQIEEAEKEFSKNK"
     gene            10921..11943
                     /locus_tag="PL056_05350"
     CDS             10921..11943
                     /locus_tag="PL056_05350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HPM46474.1"
                     /translation="MNAKKRILIVHTGGTFVMESNEQGQLKPGDYAFDYIDHTIHALF
                     DAEIVQTRLLNIDSSLFRPCHWVEIAEHINKQYDNFDGFVIIHGTDTMAYTASALSFM
                     LKNLGKPVVMTGSQLPLKNRRSDALMNLVDSIEVALFSELNEVVVLFNHTVFRGNRVK
                     KKDSWDFDAFYSPNYNSIIKLGIGMEKKPHRYLPKKPGVFEIDARLVEEVIIIPFFPG
                     IDFSTFSIMVRKKKVKGIVIEAYGSGNIPSDNPGLEELFKDAAENSVPIAVCSQSPIG
                     KVNLGLYEVASKAEYYGLISAVDMTREATLVKLMIALGRYQSIDKVKKFMMESISGEK
                     ESADVR"
     gene            11933..12865
                     /gene="era"
                     /locus_tag="PL056_05355"
     CDS             11933..12865
                     /gene="era"
                     /locus_tag="PL056_05355"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HPM46475.1"
                     /translation="MLGKELKYTGFAAIIGAPNSGKSTFLNNVVKSKVSIVTPKAQTT
                     RNKIRGVYTKDDAQIVFTDTPGITSTDFGKLLNNWMNRYSFSAANESDFTLFFVDVST
                     QHPELGIGEEEKHILDNLKSRTPTILVANKCDIVKKMRADDTIAVYRKYFKFADTFAV
                     SALDGTGIDELVEAIKKYCVSGPQLFPEDMTTDQDDSFLVSEIIREKLFMELSQELPY
                     HTVVTIERMTDHKSKDILMIDATIHVARKSQKSIVIGAKGSTLGNIGKKARLELEKIY
                     GCQVGLKLFVRVEEKWFEKERLLQKVGFEKDFDR"
BASE COUNT         3819 a         2586 c         2064 g         4618 t
ORIGIN      
        1 tcaatttccg caagagagta taaatcaaac aaaaaagtgt caagattttc gtaggagcgt
       61 atccgtatta tccgtagggg cgaaaaatct ttcgcccagt tttgttataa ttttgctgac
      121 ttgaccccta agttccgccc cccgtacgga gggcgacaaa tactttgaag caaacggacg
      181 gggctatctt ggtttcaatc cacgcccccc gtgaagaggg cgacttaatt gtctcaatct
      241 tttttggtat tttcatgctc ttcactcccc accctcaacg atttttattt cttcttctgt
      301 gagtgtttcc atcttttcgt taagaaattt ttcaatttct ttggggtcag gaaggttagt
      361 aaggtatttt gacacgaaaa gctgattatt cattcctgca agcgcatatt ctaccagtga
      421 atgattcttt tttgtacaca gtaaaatacc gacgggcgga ttatcgccat cttgcatttc
      481 atttttccta taccagttaa cataggtatt aagctgaccg aggtgttcct gagagaactt
      541 ttcaaccttt aattctatca gcacatgaca ttttaaaatt cgattataaa acaccagatc
      601 aacaaaataa tgttctccac cgataaggat tcttttttgc cgggcttcaa aacagaagcc
      661 atgtcccagc tcaagaagaa aattctgtaa attatcaagt attgcatctt ccaaattaga
      721 ctcatacata acttctttcg attttaatcc aagaaattca aagacaaaag gatctcttac
      781 agtgagttta ctgctgttcg tttcagcaat gcttttaaca tattctgaaa gtttatgctt
      841 atctttagaa agcccgcttc tttcataata tagagaacct atctgtcgtt ttagttctct
      901 gacagaccag tttcctgtaa cagcttcaaa ctcatagaac atccttttca tatcatcatc
      961 aatattgcaa agctcatcaa aatgggaata ggataacatt tcaaaaacat taaggttgta
     1021 tgtgttggaa gactgtgtgg acagcgtccg cattttttca agatcattat tttccggcaa
     1081 atttttaaat tgtgccgaca gtgtccgcac aattccggga tatgttttat aaaacttaac
     1141 atactgatag agctgccttt tcccggaatt actaacacct ttttctttca gttcattaga
     1201 aagatctaaa aagagattgt ctccgtagtt agctctgtct tcaccgttaa gttcaaaaac
     1261 atcaatataa aaacctatga gccagtttct taaagtaaga cttttattta ccgcttttga
     1321 gacggaacag gtcaaatact cgtctgtact tacaatattt aaaacaagat cattgaatgt
     1381 cctgatgttt gttgtcaatt tcgattcttc tttcattgtt tatccccacc ctcaacaatt
     1441 cttatttctt cttctgtgag tccgtaaagt tcataaacca ttgcatctat ttgtttttcg
     1501 aggtctgttg tgtcttgtcc aagctttttc ttattcataa ttatatcaac tattttggtg
     1561 aatctttttt gttcattaat atttgttttc tttatgggaa tcgattcaat tgagtccttc
     1621 ttaattttag ggaaagtttc tttgttgtca taatttgtca atatccaata acatctaaga
     1681 actttagagt taattaatgc caacaaaaat tttaaatcaa tttcgcaatt atcatttaag
     1741 aaaatattgt atattgtgtt gagtgtgaaa attcctggag aacatattgt accttccggg
     1801 aattttccaa cttgtctgat tacaatacgg tttttcttat aaacctcgtc atttccacca
     1861 cttttgattg catctttaat ataacaaaca aattctgtgt tttttttcag gttataccta
     1921 aaaacattac caccatctat tacaggcatc caattattgt cgattttcat ttctgacaca
     1981 tatgtttctc tatcatgtgt ttgtatccca aagtatgaac ggccataaaa acttaatggt
     2041 ttagtgtcta aatgaatttt tttcaaaaac ctttccttct tttcatccaa atcaaagtta
     2101 aaaatatatt cgggtttaat aattttttta ctgtcaaaaa atctaatcgg aaccgaaata
     2161 tcatttatag atttttttat ctcatatatt cttgataatg atcctttttt attttttgaa
     2221 aaaacaaata tcaaattatc aacagacaca ttctcaaaaa cctgggaata aaacaaaact
     2281 atggataata tagatgaatt aacgcaaata aattccctta atttgttgtt gtatttgttt
     2341 tttataaaag tgttaggggt aataaacgat atgcttcccc tttttgataa caacaaaatc
     2401 cctttttcaa tgaataagtg gtaaagatct gttttatatt gggcactgac aaattttttg
     2461 tatttatcaa ttgtttgatt tgagatacct tgaatgtttt ggagcaaaat ataaggcgga
     2521 ttccctataa caacatcaaa cccttctttt attccgaaca tccattcggg gtcaaaccag
     2581 tctgagcttt tattctgatc atacaggtcg aagtctgcga ttttattggc atctgtggct
     2641 ttataatgat tctgaatgag tacatctgca atttgttttc tgatttcttt gtcttttttg
     2701 atgcaggcaa gtttttcatt tcttgttttt gcggaaaaat gttcatgccg cactttgagg
     2761 agtaatttct tgagcggttc tataaaaatc tcttcaattc cgccttgatt cggtttatca
     2821 agtcctatga gtgtgttcgc tgacacaaac tttgtctcaa ggttaggaag cggtctgatt
     2881 ccgtgattat cttttcttct gtcaactttc tgatcaacga tgagcgagat aaaaaacctg
     2941 agtttgcata tctgtattgc aatgggctga atgtcaacac cataaatgca gttttcaatg
     3001 agataaagct ttcttggata atcggcttcg ttatcgcaga atgctttcat tatatccata
     3061 cgaagctttt caagctcctt tatttcagtt ttgtcagtgg tatcggatac tttcctgtct
     3121 atctttttta gctgcaactc ctcccattca ctgttgtccg gattgagttt gcttaaaaca
     3181 tgaacaactt tattaagaac tcccatgggg aaagcaccac ttccaacagc cggatcaaga
     3241 atctttattt catcaaatgc tctaactatc tttaaaactt tatctttatc ttcaaacggt
     3301 tgatcgtcat tatgtttaaa aagacgatgc agaagatggt ctgtctcttc agtttcatca
     3361 tcaagttttg ttttcagata tgcaataaga gattcatcaa ccatataatt tacaatttct
     3421 cttggagtat aaaaggaacc agtctgcttt cttgcagttg tctttgtctc cgggttatag
     3481 cttgcaagaa gattctcaaa aacttttccc aaaagttcag gatcaagtgc aacatcttct
     3541 tcagtcggag tgttttccgc aattgtgaat ttataagaat taagaatatt tataagacct
     3601 tctgcagagt atgtcttgtt ttttgttcca tatatttcat tgagatcaac ttctgtctcc
     3661 tctccgaaaa atagataatc cggaacaaat gcctgcttgc ccggatttct tgaaaaaccg
     3721 tcaacatata ttgtcctgtt cttctcattt tctttatcaa gacagtcaaa aagtccacca
     3781 tttaaaaaag gaatgtcttt gaacagtttt tcagcttcga gttttgaaat tttaaaaaga
     3841 gaaccgtatc tccaaagagt tttaactcca tattcttctc tgtttgtttc cttatcttca
     3901 tgtccaccgt cctttgcaaa ctttctttcg cccatcttct ggttcagcgt tccgaaaaac
     3961 aggttctgca aaattgctct gtaatagttg cctgactttt tgttcttatt aaaatcatca
     4021 ataatatcat ttacttcatt tttgttgaac aatttttcag gaacaagatt cttttctttt
     4081 atgaaccaga tgaagatgat tcttgtgata agacggataa gattcgttgc gtttcttaca
     4141 tctttccttt tttctccatc atcaggaaat tcaacattat ccatcgccca gaaataccag
     4201 ttggaaagct ctttataaaa cttcttgtta aggatggaaa catcaaaaac ttcctgccag
     4261 tgtttatgaa gagaagcaaa tgaatttact gtgttttttc cacttgtcgg aattttcaga
     4321 ccttcgagaa ttttcagatg tccagtgtga gtattttcaa tgtttatgtc gcgaagaagt
     4381 gtaattttac caattttttc accttctttc catttctgaa tatattcact tctttcacta
     4441 cttgcaattg tgataaagtc ctgatattta aaaacaatta ttacaggagt atatttgaat
     4501 tccctgttga aagctcttgt aatatctgca aatatctttc ttgtcggaag aagtccgttc
     4561 tctcttttcc caagtttcac tccaaaaacg gcgattgcct catatttttc agaaatgtct
     4621 gaaattttaa tggttttctg atcattgaaa gcttcgtcat tgaccaatcc ataaggataa
     4681 accgcctcaa taagtttaag agattcactt tctaaatttg cagttgaaag aatgtctttc
     4741 ggttcagctt ttaattctgt tatcggattt acaggaatat tcagatcatt gaaaagattt
     4801 ttgattcctt ctacaagacc ggtcttgaaa ctgcttaatt tcatattttt ctccgtctta
     4861 tcaaacaaca gtcaaacaaa ttcctaatat aaaacagctt gaatatattg atgaattgcg
     4921 aacacaatca ctaaaaatca ccatttttgt caaacaacag tcaaacaaat ttaagcccac
     4981 cacggtaaat attttctcgc tcttgttccg acggaatcat cgtgaatttt aatagtattc
     5041 tctatcaaag cctcttttat tattctggat attattgcac tgtttttcag atcaattttc
     5101 attctttccc ttatggatga attggtcata tattctccgc atacccattt taaacatgcg
     5161 tgctgataac atgcccttat tctttcaatt ctatccatat ctttgtattc cctgtaagaa
     5221 taaagaatta cttttgtgta atcatcttca gcctgaaaaa gcggtggcgg cagttgaaat
     5281 atttcaacct gtgcaagagc acggtcaact ccgcttcccc tttcttcgca aatattcatc
     5341 cttctcataa gtgaagcaag tttttcattt cttgttttag gcggatagtc aataaatctc
     5401 tcagtatcaa tcagtggact tcccggattt gtaatctcaa tccttgtatc aaatatttca
     5461 atcatgggac ttcttccggt ttctgtaaaa tcctgatgaa ttaaagcgtt ggcgacaaat
     5521 tctcttatag ccagcttagg atacctcttt tttccaactc tgaacgcttc ttcaataaat
     5581 tcatttgaag gaatcagatc atttatccaa tcaatcaatc ctttaaatcc ggcagcatat
     5641 ccctttttcc ctgtaatctc atttttcgca ttaaccctgc tgtttccatt ataaactatc
     5701 accctgacag ctttccttga caagtgctca aaatcatgga ggttttttgc gatcaaaagt
     5761 gcaccgagat ttgttattga ataactgtta tcatcctgta ttacaattcc atcctgcaca
     5821 agctcttcaa ttattctgtt ttggttttcc ggaacaggaa tgttctgaag tttgaaaaaa
     5881 ttatgataat caattttgtt tagaacatct gcaagagaca ggttagaagc cgcaacagct
     5941 ttttcaaaag agaccgattc agcttttttc catattttgc gctctttttc cgggtgatca
     6001 ctcaatttgt gtttatgtcc accgacacga ataaacgctt ctccatcaaa tttcacaggt
     6061 gtatttaaag tcgcatcaat ttcaaaaacg acaatatgtt tttcatcctg atcaaattca
     6121 attactttaa aatcaatcct cggagagagt tttcttgaaa gccacggttc caagtcttca
     6181 tttccttttc cttttgtgtt tgaaggtaaa aaattcgttc caacgatctt ccctgttcca
     6241 ttctcaatac cgaatattaa aaatgccttg tttttcccgt gaagagcggc tgaattactt
     6301 aatgcggata aatattctcc aattaattga gggttacagt tattttcttt aaattctatc
     6361 cattcatttt ccttgggaga tctgaaataa tcctctataa tctttaagta tctattcata
     6421 aatgcacctc tttattcaat gaccatccat gcaataagat catagttttc aaagcaatat
     6481 ctctgttcta tggctttttt tccttttaca tcttttattg cttcacagtt tccggatttt
     6541 atcttttcaa gaatatctgt tccttttttt ccgagcactt tttccccatt ttcatccact
     6601 tcgtatgcct gtgactcaac ccagcttcta attgccattg aaagtttttt tattttttca
     6661 ccatttccgt tgtctatctc tttatcaaca tgtctttgtt tctctttgtt ttcagacaat
     6721 gctcttaaaa tatctttctg gtttaataac agtgagttac cgttttcatc tatataaaca
     6781 agttcatgtc tttcatattt gaatttctta tctcctgatg gccttgaagg tgatccaaga
     6841 agtgcaacca atccattttt tttgcagaat tcaccatctt taataaaccc tgaataaaca
     6901 ccattaggca ttttaatgta tttttctttt tcatcttcaa gatatgaaaa cagttcctgc
     6961 ctgaaagatt caagagaaaa gtcatcaaaa ccaagtcctt cactgttttt ctgcacttca
     7021 tcccacgaag attccatcag tttcatcatt ttggcattct gtctctgctc aagtgtttca
     7081 tctttaagca tttcttcgtg ctctttagaa aatgcaggga cctcagaacc ggcaagagtc
     7141 attgcagcca ttttatccgc cactcttttt tgtagatcaa gatagctttc tattgattca
     7201 gaaggccaga aattcactcc ttttattttt ttattcggtg atcctattct gtcaattcta
     7261 cccatcctct gaatgattct tactggattc cagtgtatat cataatttac aatataatca
     7321 caatcctgaa gattctgacc ttcgctcaaa caatcggttg cgattagaat atcaacagaa
     7381 ttatcaatct ttttatatac ttccggctta ttctcttttg tccattcaat ccacaaattg
     7441 aagttttgaa catctgtttt tttattatct ctttcaaatc caacccactc tttttccatg
     7501 aataacttcg taaatggagc gaacctttca agaaccggtt caaaaaactt gccgttgtat
     7561 ccatcagatg attttgtttc atctcccgac accattgcaa ctttttgaaa tcctctttta
     7621 gtgagctcat tataaagata tgtcgcagta tctttgaaaa ctgtgaatat caaaaccttt
     7681 ttattattag aattttcctg ttttttcaga attaactgaa taagtttttt cagcttcgag
     7741 tcaacagagt ctttccctgt ctctttttca acgatctctg aaaatcttct tacatttgtt
     7801 attaaagaat caagcttgtc aatatccttt ttgaggtctt ttttaagaag atcaagtttc
     7861 ccggctctat ctatttccga aatgttcaca gttctttttt taccaagagt gaactcttca
     7921 aattcagcat cttcttcttc gtcaaaaata gtcatttgac cataaatatc aatatcttca
     7981 gacttaacct tgtcgttata ctgctttatg gtattaagca cttcctgatg gtgttcactt
     8041 atcttttcaa cagttgattt aaatgaaaac catgacgact caagccgctt tataaggaga
     8101 atatacatca ttttaacaag aaaacggtct ctttgttttt catcttcaat aacattttca
     8161 acttcttttt cttcaacata ccatgccggc tgatagcctg acatatatgg aaaatggtca
     8221 agcagttctt tgaaactctc aatatttcct atctcttttg gtgtaatgaa tatgttttcg
     8281 ggaagttctt tctcggggaa attaagcatt ttctcattaa tagactcaac atgttttctc
     8341 gttctggaaa caagaagaga atcaatcagc ttaaaaaaat caggatttaa tttttttatg
     8401 aaatcagata ttctgcctgt tccttcttca gaccatttgt tgaattcccg ctgtgcattc
     8461 ctgaaaagat gcatcagatt tcttatcccc aaattttcag tgaaaccagt gtctcctcct
     8521 tttacaatta atctgaactg gtttcttatg tcattaagag agttgtttat aggagttgca
     8581 gaaagcatca acaccttaac ggttttgttc tttttcaaaa gatagtctat caaaaattga
     8641 taccttgaag acctctcgtt tcttaaattg tgactttcat caattacaaa taattttggt
     8701 ctgtcactct gaaaataatc ttcaattgta agcccatcct gatatctttg aagtctgtca
     8761 tcctgaagat cagtgtgata tcttattgta tagttaagtc tatcctgctc aaatcttgaa
     8821 ttatgcttgt ggagaaactt tctccagttc tgatcaagtt ttttgggaca aaaaagaata
     8881 acttcatatc cttccatttc ataaaacttc attacagcaa gtgctgtcca agttttacca
     8941 agaccaacag catcagcaag aatggctccg ttgtatttct gtatcatttt cacaagactg
     9001 ataacagcgc ttttttgaaa atcaaacagg gaattgtaaa gaacggtatt ttctaccctt
     9061 ccgattttct tattaaaatc agggctttcg acctcgtctc cgatgtcttt ccagaaaagt
     9121 tcaaaaagta ttttgtaata tatttctttg ggcaaatatt ctctgaaaac cttttctatt
     9181 tcttcaataa tatactgttt gaaatctttc tctgtttttg ttccgtcagc atgaattatt
     9241 gtttttttgg agtgagcttt tttagacttc caaatatcat taaaccagat tttaagctct
     9301 ttaaattgat ttccctgtcc tgtttctgca atattcagct caacatttcc tgttattttc
     9361 aaaccaattc cggactcagt aagatttgag ctgcctgaaa taaaataatc atgcctatca
     9421 tctttttctg atttaaaaag atatagtttt gcatgacaga aatttggttc aagcgtctta
     9481 agttcgactt tttcctgttt taggaattca acagcctctt tagatatttt tgaaagtttt
     9541 aaagctgctt caactgaaat gttttcattt aaaagatcaa ttgctctgtc ttcgattccg
     9601 ccggtactta caatgtcccc aagtataaat ctcatatctt taactttttc gttaattact
     9661 ttagaaatat ggttgagggc tccgatcgta aaataacccg ttacacagtc aaaaaaacca
     9721 ttttctgtgt attgttcaat ccattcataa acttttttgt tatcgttact gttgtctaat
     9781 agcatttttt cctctcttct tcaacaagaa tgcagataat ataatcaaac tgaatcgaat
     9841 agtaaataaa agttgaccgg cgtttcttaa tgtttccctg tgttcggttg aagataattt
     9901 agtgtagggt caaatccgtg tgttcgcccg tgtaaatcca ctcatttatt ttttgaaaat
     9961 tctttttctg cttcttcgat ctgttttttc agttcttctt tgcttggaag gtagagctgg
    10021 taattggatg cataaatatt gcggtctttc ggtaatgtca actccacgag agaatcactt
    10081 tttgtgagac acagcagtat tccgactgtc ggattttcct cttctgtttt cacataacgg
    10141 tcaaaataat ttacatacat ctgcatctgt ccaagatcct gatgtttcag atctccgatt
    10201 ttaagatcaa ttacaacaaa acatttcagt agccggttat aaaacacaag atcaacatag
    10261 aaatgtctgt tatcaaaaga aaaccgtttc tgcctcgatt caaaaagaaa tcctttgcca
    10321 agctcaagaa taaaaagttc aagtttatca ataattgctg tttcaagctc gtgttcggtg
    10381 tattttttct gcggctccag acctgtaaat tcaaggatat atggattttt aattatatct
    10441 gctggttttt ctacaatttg tccctttatt gacagctctt tcactccgtt tttatcgcga
    10501 ctaagcacca atctttcaaa aagagcggaa tttatctgtc tttcaagctc tctatatccc
    10561 caaccatttt ttgctgcttc aatttcatag aatcttcttt cttcatcatt ttttactgtc
    10621 ataagagtca cataatgtgt ccagccgaga acaaaccttg aagagagata ttctatagta
    10681 ttttgcattg tttccggaaa cttatccaat tcggcaggca gtgcctgttg aatcttgtaa
    10741 ttctgataaa actctctgaa ttttcgcaaa tttgttggag acattccttt tattccgaag
    10801 gttgaaagac tctcagacaa aatatcaatt tatttagtga gtcgtctttt caacttatga
    10861 atcttgcaat aagccgcaat ataagtaaaa ataatgcgtg ttgataagat ggagttagag
    10921 atgaacgcaa agaagaggat cctgatagtt cacacgggcg gaactttcgt gatggagtca
    10981 aatgagcagg gacagctcaa gccgggtgat tatgcttttg actatattga ccacacaata
    11041 catgctcttt ttgatgctga aatagttcag acacggcttt taaacattga ttcatcactt
    11101 ttcaggccat gccactgggt tgagatcgct gaacacatta acaagcagta tgacaacttt
    11161 gacggttttg tgataattca cggaactgac acaatggcat acaccgcttc ggcgttgtcg
    11221 ttcatgctta aaaacctcgg caaacccgtt gtcatgaccg gctcccagct tcctttgaaa
    11281 aatagacgga gcgacgctct gatgaacctt gttgattcaa ttgaagttgc ccttttcagc
    11341 gaacttaatg aagtggttgt ccttttcaat cacactgttt tcagaggcaa ccgcgttaag
    11401 aaaaaagatt cgtgggattt tgacgcattc tacagcccca attacaactc gataatcaag
    11461 ctcgggatcg gaatggaaaa gaagccacac agataccttc cgaaaaaacc gggagtattt
    11521 gaaattgatg cacgacttgt tgaagaagtc atcatcattc ctttcttccc cggcatagat
    11581 ttctcgactt tctcgatcat ggtaagaaag aaaaaggtta aaggaattgt cattgaggcc
    11641 tacggatcag gcaacattcc aagcgacaat cccggacttg aagagctttt caaggatgcc
    11701 gccgaaaatt cagtgcccat tgcggtatgc agtcagtcac cgatcggaaa ggtaaatctc
    11761 ggactttatg aagttgcgtc aaaagctgaa tattacggac ttataagtgc tgttgacatg
    11821 acaagggagg caactctggt taaacttatg atcgctcttg gaagatatca gtcgatcgac
    11881 aaagtgaaaa aattcatgat ggaaagcatt tcaggcgaaa aagagagtgc cgatgttagg
    11941 taaggaacta aaatataccg gctttgccgc aataatcggc gctccgaact caggaaaatc
    12001 aacattcctc aataatgtag taaaatcaaa ggtttcgata gtcactccga aagctcaaac
    12061 tacaaggaac aagatccgcg gagtttatac aaaagacgat gcacagatag ttttcaccga
    12121 cacacctggg ataacttcga ccgattttgg caaactcctt aacaactgga tgaacagata
    12181 cagcttttct gccgcgaatg aatctgattt caccctcttt ttcgttgatg tttcaactca
    12241 gcaccctgaa cttggaattg gtgaagaaga aaaacacatt ctcgacaacc tcaaaagtag
    12301 aaccccgacc attcttgtcg ccaacaaatg cgatatcgtt aaaaaaatga gagctgatga
    12361 caccattgcc gtttacagga aatatttcaa attcgctgac acatttgcag tttcagcgtt
    12421 agacggaacc ggaatagatg aactcgttga agcaataaaa aaatactgcg tttccgggcc
    12481 tcaacttttt cctgaagata tgacaacaga ccaggatgat tcattcttag tatctgaaat
    12541 aattagagaa aagcttttta tggagctttc gcaggaactc ccctaccaca ccgttgtaac
    12601 gatcgaaagg atgaccgacc acaaatcaaa ggacatcctc atgatcgatg ccacaataca
    12661 tgttgcaaga aaatcccaga aaagcatcgt catcggcgca aagggctcaa cactcggcaa
    12721 tatcggtaaa aaagcacggc ttgaacttga aaagatctac ggctgtcagg tcggacttaa
    12781 actgttcgtc agagtcgagg aaaaatggtt tgaaaaagaa cggcttttgc agaaagtcgg
    12841 ctttgaaaag gattttgaca ggtaaatatc cgttactctt ctttttgttg acaattagac
    12901 acagcttata ttaaacttca ccgttttaac tctatttaca gaggtttcgg aaatgaaaaa
    12961 gaatcttttt gtgattattc tgtccggatt aattttattc acctcctgcg aactccagaa
    13021 gtttgaaaga catgttgcgg aaaatgatga agaacagtcg gaatcagatg atgaaatctc
    13081 tgaaaat
//
LOCUS       DAOOYB010000063        12984 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_4933_length_12984_cov_9.714904, whole genome shotgun sequence.
ACCESSION   DAOOYB010000063 DAOOYB010000000
VERSION     DAOOYB010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12984
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4933_length_12984_cov_9.714904"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..1270)
                     /locus_tag="PL056_05360"
     CDS             complement(11..1270)
                     /locus_tag="PL056_05360"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HPM46476.1"
                     /translation="MSKNIENKLVPKLRFPEFLNEANWVEDTLVNIAGFRRGSFPQPY
                     GLPKWYDDINGMPFIQVYDVGDDFRLKPQTKNKISKIGAEQSVFISKGTVIITIQGSI
                     GRVAITQYDAYIDRTLLLFEEFYIELDKTFFAYLLFLLFEIEKLKAPGGIIKTITKEV
                     LSQFVVKLPSPKEQQKIAACLSSLDEVIAAENQKLDILKDHKKGLLQNLFPQEGETVP
                     KYRFKEFENSGEWVEKTLGEIGEPLMCKRIFKEETTPNPNNGIPFYKIGTFGRVADSY
                     ITKEIYEEYKSKYSFPKIGDILISASGTIGRLVIYDGSPAYFQDSNIIWLGHNEKQVL
                     NSFLFYCYSNLEWQTSDGGIISRLYNSDFKRMTIFFPKNQKEQDKIADTLSSIDDLIN
                     AQNQKLETLKLHKKGLLQGLFPGVNET"
     gene            complement(1263..4241)
                     /locus_tag="PL056_05365"
     CDS             complement(1263..4241)
                     /locus_tag="PL056_05365"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016200262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HPM46477.1"
                     /translation="MIKETQIENSLIAKLTDLKYTYRSDIRDRDALEKNFREKFQALN
                     KVNLTDSEFTRLKDEIINADVFASSKRLREINTFIREDGTPLHYSLVNIKDWCKNEFE
                     VINQLRINTENSHHRYDVILLINGIPVVQIELKSLEVSPRKAMQQIVDYKSDPGNGYT
                     NSLLCFMQLFIVSNRANTYYFANNNDAHFNFNTDEQFLPIYQLASKENKKITHLDDFS
                     EKFLSKCTLGQMISRYMVLVASEQKLLAMRPYQIYAVKAIVDCIHENRGNGYIWHTTG
                     SGKTLTSFKASTLLKDNPDIEKCLFVVDRKDLDRQTREEFNKFQEGCVEENTNTETLV
                     RRMLSDDYANKVIVTTIQKLGLALDPNNRNNYKERLEPLRKKRIVFIFDECHRSQFGD
                     NHEAIKEFFPNAQLFGFTGTPIFDDNAIYKQIDGNIGSYKTTKDIFQQSLHQYTITHA
                     IEDKNVLRFHIDYFKPEQNITIGSIQHKTAVANAIIKKHDAATHSKRFNALLATASIN
                     DAISYYELFKNMQAKRSKEDENFIPLNITCVFSPPAEGNKDVQQLQEDLQQEKEDNKV
                     EPEKKKKALQAIIGDYNLKYGTNHGINEFDLYYQDVQQRIKFQKFSNTDYPHKNKIDI
                     TIVVDMLLTGFDSKYLNTLYVDKNLKFHGLIQAFSRTNRTLHSTGTEVTIKPYGNILD
                     FRQQQAEVDRAIALFSGEDTGRAKEIWLVDSAPKVIKKYAEAVKAMEKWMESKDVVAE
                     PQAVYNIKGDTARVEFINRFKEVQRLKTQLDQYTDLNEDQKAIIKELMPEEQLRSFRS
                     SYLELAKQLKEVQQKEGDKAPENIQQLDFEFVLFASAVIDYDYIMNLIAKYTQGTSKK
                     QKMTRDQLISVLSSSANLMEEREDIIDYIKTLEVGKGLNEHEIRNGFQQFKAEKIAKE
                     LTEIAQKNGLELSALLKFTENIINRMIFDGEKLSELFAPLDLSWKERTQRELALMEEL
                     TPLLKKQAEGREISGLKAYE"
     gene            complement(4354..5103)
                     /locus_tag="PL056_05370"
     CDS             complement(4354..5103)
                     /locus_tag="PL056_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016196388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone oxidoreductase"
                     /protein_id="HPM46478.1"
                     /translation="MINSIKMRILQGDPIVHDVRKNPMSPVYRGFPIISDAKCADNCT
                     ACFDICPVKAISLDPVSIDLGKCVYCPECEKICPAEKIHFSQNYIMSATERGSLVIKN
                     GMTEIKPETASEKIRKYFGKSLKLRQVSAGGCSGCELELNAAGNINFDMGRFGIEFTA
                     SPRHSDGIVITGPITKNMAKATEICLEAVPNPKIIILNGACAISGGIFQNSTEIDRSF
                     LEKHKIDLYVPGCPPHPLTFVTALLNWLDRK"
     gene            complement(5119..6597)
                     /locus_tag="PL056_05375"
     CDS             complement(5119..6597)
                     /locus_tag="PL056_05375"
                     /inference="COORDINATES: protein motif:HMM:NF012566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HPM46479.1"
                     /translation="MTSINVHNNEPVSLSSIKFLDKVTFSQSVKADLASGKRMIGLLP
                     ARKNDPSKIIAVLADPSSSLISIAGCDFKNGPLEFESFANEYPHTNFFECELSENHAF
                     IPLNHPWMRPVRKQNVIFGKTPYEFYRITGEEIHEVAVGPIHAGVIEPGHFRFQCHGE
                     LVYNLEINLGYQHRNIEDLIIKSNSVQRLILAESIAGDTVMGNMNAHCNAMEGLSGTV
                     PDKRAQIIRFIGEEIERIAMHIGSLSGIATDVGFSLAANSYGRIRTIMINSLALICGS
                     RFGRGLFTYGGVRFDITDNIIKTLKDNLKFVADDILKINKYMFSSVGALSRFETTGKV
                     SSEDAETVGLVGVAGRSCGIRKDTRVDFPYGAYKFIPVSIITMNSGDVYARAFIRSLE
                     INESFRHILEQLEKLPQGEISSTMKDPAPDSLIISITEGFRGEIVHIACTDSSGGLVQ
                     YKIKDPSFNNWYGLSIALRNTAISDFPLCNKSFDLSYAGHDL"
     gene            complement(6594..8048)
                     /locus_tag="PL056_05380"
     CDS             complement(6594..8048)
                     /locus_tag="PL056_05380"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HPM46480.1"
                     /translation="MTAILVMLTLPFMAAVTSFLFKNWKIQYAVTLLSAFLHLTVSFA
                     IFTGFYIPEQFFMFGYDSLSKLFLVVLSNTYFWIVLVSYSYIKLPVESDSTSGKKYYF
                     MLMNFYIIANTAAILSNHFAMYWVAAEATTLSVAPLVYYSRSQESLIAMWKYLFIVSV
                     GIAFAFIGILFLALSSKGTVLDGQQLYFSEFIKNAHLLSPVWLKAGFVFIFVGLSTKI
                     GVAPMHAGDVDATSTAPSPIAALFSGTLRITALLGVLRMLQILKNTGSYEFAKFLLVT
                     GGLLSILVTLIFMLKATDMKKMVAYSSVEHLGIIILGIGTGGLAFIGAMYHIVFNSMT
                     KVGLFLCAGKIHHKYNSKNISEISGVLERLPWTGWLFLISFFAVSGIPPFGFFFSEIK
                     IFEGMLFSQRPYILLITMFMFLLIFINMGNLFFKMLYRKDPQKEFVSNDEKFDVFHFT
                     VISLMLIMIAVAVATPDVLSKNILDIANDFGMLP"
     gene            complement(8051..8680)
                     /locus_tag="PL056_05385"
     CDS             complement(8051..8680)
                     /locus_tag="PL056_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46481.1"
                     /translation="MQNVLSILFCLSLFYLVVTTSVKSYISVLRMQGVLLVILMILPF
                     IPDFSIFGLILPLTMLVVKVILIPRYMEKIMNDLNIKREIESTLQQFNFLLLSCFSMA
                     VIFIVSNYLSKSTEFAVIPFATAFSAVTIGIYIIMFRKKLIVHVIGFLVLENGIFMFG
                     LAVASEFPIIIELGSLLDVFAVVFLMGIAINKISSTIFTDDRLAQRRDP"
     gene            complement(8689..9615)
                     /locus_tag="PL056_05390"
     CDS             complement(8689..9615)
                     /locus_tag="PL056_05390"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HPM46482.1"
                     /translation="MELIEIVQGLISFFLILAFSPLFAGIVNKQKALFIGKKGAPVLQ
                     PYYELIKLFKKETINAGDSSFISVFSPLINFTSVTVAAAILPIGLWQPLISFEGDIIL
                     FAYLLGLARFFQILAAMDIGSSFEGMGASREALFAVFAEPIFFFTVGSIAFISGHTSI
                     SGIFHSVSLNNISVIVFVVVCSISSFILAVTECSRMPVDDPNTHLELTMIHEVMILDN
                     SGIDLFFYQISSYIKLFIYSVIITSFFYPFSVENYWLALLVFLVVMFAISSVLALTET
                     VTSRFRMKNIPQYLLFATAIGILNLLIFTFSE"
     gene            complement(9606..11561)
                     /locus_tag="PL056_05395"
     CDS             complement(9606..11561)
                     /locus_tag="PL056_05395"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HPM46483.1"
                     /translation="MESLFFPGLAVQMTALIFAILIKDRKKSLILSQVILIIGIIISF
                     LPVLISLLPGHAGINISYNGMFRFDGLTKFFLFVIQIIAVPTSIYNLSYFDHYIKKGH
                     SAASSIVFFIILVVACQFIVISNHAIMFLISWEIMGTSAYGAMLFEKEKPDVQKGSFY
                     YFVASHVLVFIMYIAFFLLHHESGSWFFTDFDLSGSESSIVAAIFLLSFAAFGIKAGF
                     IPFHFWLPRAHPVAPTPLSAFLSGIIIKLGIYGIMRTILFLPSVSEWMGWFVLAVSMV
                     SAIFGVWYALAQHDLKKLLAYHSIENIGIIGLGIGIGLIGSANNSIGIQVLGFGGALL
                     HTLNHAIFKSLLFTGSGIIYQNLGTRNIESMGGIVHHSKFFTLLFIIGSVAISGIPPL
                     NGFISEFIIFNGFFMISNELKNDYSLAMIVSVVGLALVGGLAVACFTKINSIMLLGSE
                     RKKIVSFKVSMAEYISLGFFAILCIFIGFYPQPVLWIINGVLEEGFLPAGKGTLFSIK
                     WGYINLVCISLTAGVAVLFVLRRILKKKYGGRRSAAWGCGYDLLTPKMQYTASSYADE
                     LNIIPKALLVYCKKYQDHDSVFPVETGFHSHSDDFIDTKIVLPVYRKSVAIIAKFDYF
                     SRSDVRYYVAFILGSIAIYTMVAFLWN"
     gene            complement(11650..>12984)
                     /locus_tag="PL056_05400"
     CDS             complement(11650..>12984)
                     /locus_tag="PL056_05400"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HPM46484.1"
                     /translation="YTNDEHGHIYEKDGWYKGSVLYEMWEEEEKNCRGCNVFRISGGD
                     NYTGTAISSLFNGEPVAKIMGLTGYRISALGNHEFDFGTLPFENNRKISGIEYLSSNI
                     ISSDLKNAFEPSLIYPFKDGRISFIGATTEELKQISFASYLKNLRVVKPLSTVGRELK
                     KNEPLSDFQVVIVHESAETAKDWVKDLPVKPLIVFGAHEHVRIVKNYNDVLFVQTEGY
                     LKSYARVEVQKKGKSVTVTKVDVIPLRKKADFSSDTSKKIKDITDSYLEKLDKKAGQR
                     LITSEKDFEFESFQKLYACSLLCSFPQYDAAMSNPGAFRDVIDCGPVKKSDIISMLPF
                     QNRIVISTLKGEDLIYNLNLSQESYCGAIKKGNDWFIKGEPVQKERKYTAVIHEYIYS
                     GGDYYKFILDDSNNEITSRDWREPLEKYLAEASSKGMNLEEAYADLMAKFNR"
BASE COUNT         3800 a         2619 c         2516 g         4049 t
ORIGIN      
        1 aatttgttac tcatgtctca tttactcctg gaaacaaacc ctgcaataaa ccttttttgt
       61 gcaatttcag ggtttctaat ttctggtttt gggcattgat taaatcgtct attgatgaaa
      121 gtgtgtctgc aattttatct tgttctttct gatttttagg aaagaaaatg gtcattctct
      181 tgaaatccga attatacaat ctacttatta taccgccatc agatgtttgc cattctaaat
      241 ttgaatagca ataaaataag aaactattta gaacttgttt ctcattgtga cctagccaaa
      301 tgatgtttga atcctggaaa tatgcaggtg aaccatcata aattactaat ctacctattg
      361 tgccagacgc agaaattaat atgtctccaa tttttggaaa agaatacttg cttttatact
      421 cttcataaat ctctttggtg atgtaggaat cagcgacacg gccaaatgtt ccaattttat
      481 aaaatggaat gccattattt ggattgggag ttgtttcttc tttgaatatc cttttacaca
      541 ttaagggttc accaatttca cccaaagttt tctctaccca ctccccacta ttctcaaact
      601 ccttgaaccg atacttaggt acggtttctc cttcctgtgg aaaaagattt tgcagtagtc
      661 cttttttgtg gtctttcaga atatccaact tttggttctc ggctgcgatg acttcatcta
      721 atgacgaaag acaggctgca attttttgtt gctcttttgg agaaggtaac ttaactacaa
      781 attgactcaa tacttctttc gtaattgttt tgatgattcc tcctggtgct tttagtttct
      841 caatttcaaa aagtagaaat aatagataag caaaaaatgt tttatcaagc tcaatgtaaa
      901 attcttcaaa aagtaaaagt gtcctatcaa tataggcatc atattgtgta attgccactc
      961 tgccaatcga gccttgaatt gtaattatta cagttccttt tgaaataaat acactctgtt
     1021 ctgctcctat tttacttatt ttatttttag tctgtggttt taatctaaaa tcgtcaccaa
     1081 catcatacac ttgaataaaa ggcataccat tgatgtcatc ataccattta ggaagtccat
     1141 aaggttgtgg gaaactccct cttcgaaaac ctgctatatt tactaaagta tcttcgaccc
     1201 aattagcttc attcaaaaat tcaggaaatc ttaactttgg cactaattta ttttcgatgt
     1261 tcttactcat atgctttcag tcctgatatt tcccgtcctt cggcttgttt ttttaatagt
     1321 ggtgtcaatt cttccatcaa tgccaattcc cgttgggtgc gttctttcca gctcagatct
     1381 aaaggtgcaa acagttcact caacttttcg ccatcaaaaa tcatccggtt tataatgttt
     1441 tctgtgaatt ttagcaacgc tgaaagctcc aagccgtttt tctgtgcaat ttcagttaat
     1501 tctttggcta ttttttccgc tttaaattgc tggaagccgt ttcttatttc atgctcgttt
     1561 aagcccttgc cgacttccag tgttttgatg tagtcaataa tatcttcccg ttcttccatc
     1621 aggttggcac ttgaactcaa cacactaatc aattggtcac gagtcatttt ctgctttttc
     1681 gatgtgcctt gtgtgtattt ggcaatcagg ttcatgatgt aatcgtaatc tatcacggca
     1741 gaagcaaaca acacaaattc aaaatcaagt tgctgaatat tttcgggtgc tttgtcgcct
     1801 tctttttgct gaacttcttt gagttgttta gcaagttcca gatacgaact gcggaacgaa
     1861 cgcaattgct cttcaggcat cagctcttta attattgctt tttggtcttc gttcaggtcg
     1921 gtgtattggt caagctgtgt tttcagccgt tgtacttctt taaaacgatt gataaactcc
     1981 acgcgggcag tatcgccctt gatgttgtaa actgcctgag gttctgcaac aacatctttc
     2041 gattccatcc atttttccat ggcttttacg gcttccgcat atttttttat cactttcgga
     2101 gcagaatcaa ccagccagat ttctttggct cgtcctgtgt cttctccgct gaataaggca
     2161 attgctctgt ccacctcggc ttgctgctgc ctgaaatcaa gaatgtttcc gtatggcttt
     2221 atggtcacct cagtccctgt ggaatgcaag gtgcgattag tgcgactgaa tgcctgaatc
     2281 agtccgtgga atttcagatt cttgtccaca tacaaagtat tcaggtattt agaatcaaaa
     2341 cctgtaagca acatatccac tacaatcgta atgtctattt tgtttttgtg cggatagtcg
     2401 gtattgctga acttttgaaa ttttatgcgt tgctgcacat cctgataata caaatcaaat
     2461 tcgttgatgc cgtggttcgt tccatatttc aagttgtaat cgccaataat ggcttgcagg
     2521 gcttttttct ttttttcagg ttcaactttg ttgtcctctt tttcctgctg taaatcttcc
     2581 tgcaactgtt gcacatcttt gtttccttct gcaggaggag aaaacacaca agtaatgttt
     2641 aaaggaataa aattctcatc ttctttgctt cgctttgcct gcatgttttt gaatagttca
     2701 taatatgaaa tagcatcgtt aatagaagcc gttgcgagca aagcgttaaa ccttttgctg
     2761 tgggtagctg catcgtgctt tttgatgatg gcattggcaa ctgctgtttt gtgctgaata
     2821 ctgccgatgg ttatattttg ttccggcttg aaatagtcaa tatgaaaacg caatacattt
     2881 ttatcttcga ttgcgtgggt aatggtgtat tgatgtaatg attgctgaaa aatgtctttg
     2941 gtagttttat aagaaccgat gttgccatct atttgcttgt aaatggcatt atcatcaaat
     3001 atgggagtgc ctgtgaaacc gaataattgt gcattcggaa agaattcttt tatggcttca
     3061 tgattatcac caaattgtga acgatgacac tcatcaaaaa tgaacactat tcgttttttg
     3121 cgaagtggtt caagtcgttc tttgtagttg ttcctgttat ttggatctaa agccaatcct
     3181 aatttctgaa tggtggtgac aataactttg ttggcataat cgtcagataa catacgcctt
     3241 actagtgttt ctgtgttggt gttttcttct acacaacctt cctgaaattt attaaactcc
     3301 tcacgggttt gacgatcaag atccttgcgg tcaacgacaa acaaacattt ctcaatgtca
     3361 ggattgtctt tgagcaaggt agatgcctta aaagatgtca gcgtttttcc gcttcctgtg
     3421 gtgtgccaaa tgtagccgtt ccctctattt tcgtgaatgc agtcaacaat agcttttaca
     3481 gcataaatct ggtaaggtcg catcgccaac aatttttgct cacttgcaac caaaaccatg
     3541 tatctgctta tcatttgccc caatgtgcat ttgctcagaa acttttcaga gaaatcatct
     3601 aagtgtgtga tttttttatt ttctttactt gccaactgat atataggcaa aaactgttca
     3661 tctgtattga agttgaaatg tgcatcgttg ttgttggcaa agtaatatgt gttagctcgg
     3721 ttactcacaa taaataactg catgaaacaa agcaatgaat tggtgtagcc gttgcccggg
     3781 tcgcttttat aatccacaat ttgctgcatg gctttgcgtg gactgacttc taaacttttc
     3841 aattcgattt gcactacagg aattccatta ataagcagaa tcacatcata gcggtggtgg
     3901 ctgttttctg tgtttatccg cagttgattg attacctcaa actcattttt gcaccagtct
     3961 ttaatattta ccagcgagta atgcaaaggc gttccgtcct cacgaataaa agtattgatc
     4021 tctcttaatc gttttgaaga agcaaaaaca tcagcgttta taatttcatc ctttaatcgt
     4081 gtaaattcag aatcagttaa attaacctta ttgagtgcct gaaacttttc acggaaattc
     4141 ttttcaagag catccctgtc ccttatgtca gacctgtaag tatatttaag gtcagtcaac
     4201 tttgcgatta agctgttttc tatttgtgtt tccttaatca ttaaattttc acctcaaaca
     4261 ctccaacact catttataag ccttgcatat ccgacagaat actataaaaa ttaacacatt
     4321 cggtcaagga aagtggcaga atttgtctgt gtattatttt ctatccagcc agttaagaag
     4381 tgcagtgacg aatgtgagag gatgtggcgg gcatcccggg acataaaggt ctattttgtg
     4441 cttttcaagg aagcttctgt ctatttctgt ggagttctgg aaaattccgc ctgagatggc
     4501 acatgctccg ttaaggatta tgatctttgg attgggaact gcttcaaggc atatttcagt
     4561 agctttggcc atattttttg tgataggtcc tgttatgact attccatctg aatgtctggg
     4621 tgaagcggtg aactcaatgc cgaatcttcc catatcaaaa tttatgttcc ctgccgcatt
     4681 aagttcaagt tcgcatccgc tgcatccccc tgctgaaacc tgtctgagct tcagtgattt
     4741 cccaaaatat tttcttattt tctcgcttgc ggtctcaggt tttatttcag tcataccgtt
     4801 tttaatgaca agagatcccc tttctgttgc cgacataatg tagttctgtg aaaaatggat
     4861 cttttctgcc ggacagatct tttcacattc agggcagtat acacattttc caagatcaat
     4921 gctgacagga tcaagtgata ttgctttaac agggcagatg tcaaaacatg cggtacagtt
     4981 atcggcacat ttagcgtcgc tgataattgg aaaaccgcga taaacaggag acatcgggtt
     5041 ttttcttaca tcatggacga tcggatctcc ctgaagtatc ctcattttta ttgaatttat
     5101 catatcacgg ctcctttttc acaggtcatg tccggcataa gagagatcaa agctcttgtt
     5161 gcacagagga aaatctgaaa tggctgtatt tcttaaagca atgctcagcc cgtaccagtt
     5221 gttgaaagac gggtccttta tcttatactg gaccagtccg cctgaactgt ctgtgcaggc
     5281 aatgtgaact atttctcctc tgaacccttc tgtaattgaa attatcagtg aatctggagc
     5341 cggatctttc atggtggaac ttatctctcc ctgcggaagt ttttcaagtt gttccaatat
     5401 gtgcctgaaa gattcgttta tttcaagact tcttataaaa gctcttgcat aaacatctcc
     5461 gctattcatt gtgattatgg aaacgggaat gaatttatat gctccgtaag gaaaatcaac
     5521 ccttgtatct tttcgaatcc cgcatgatct tccggcaaca ccgacaagac cgacagtttc
     5581 cgcatcttct gatgaaactt tcccggtagt ttcaaatctt gaaagtgcac cgactgatga
     5641 gaacatatat ttatttatct ttaaaatgtc atctgcgaca aatttaaggt tatctttcag
     5701 tgtttttatg atgttgtctg tgatgtcaaa tctcactccg ccgtaagtaa aaagtcctct
     5761 tccaaaccgt gatccgcaga ttagtgcgag gctgtttatc atgatagttc ttattcttcc
     5821 gtatgaattt gcggcaagcg aaaatccgac atctgttgcg ataccggaaa gagagccgat
     5881 atgcatggcg atcctttcga tctcttcgcc tataaacctg atgatctgtg ctcttttgtc
     5941 aggcactgtt cctgacagcc cttccatcgc attgcagtga gcgttcatgt tgcccatcac
     6001 agtgtcacct gcgatggatt cggcaagaat gagccgctgt acagaattac tttttattat
     6061 caggtcctct atgtttctat gctgatatcc gagattgatc tcaagattgt aaacaagttc
     6121 tccgtggcac tgaaatctga aatgacccgg ttcaataact cctgcatgta tcggccctac
     6181 cgcaacttca tggatctctt cacctgtaat tctgtaaaat tcatatgggg ttttcccgaa
     6241 gatcacattc tgttttctca caggtctcat ccatgggtga tttaacggaa tgaacgcgtg
     6301 gttctctgag agttcacatt caaaaaaatt tgtatgagga tattcgtttg caaaactttc
     6361 aaattcaagc ggcccgtttt tgaaatcaca tccggcaatg gagatgagtg aagaagaagg
     6421 gtctgcaagt acagcaatga tctttgaagg atcatttttc cttgccggaa gaagtccgat
     6481 catccttttt cctgaagcga gatctgcttt tacactctga gaaaatgtta ctttatctaa
     6541 aaattttatt gatgacaaag ataccggttc attgttgtgt acattaattg aagtcatggc
     6601 agcatcccga aatcattagc gatatccaat atattttttg aaagcacatc aggtgttgca
     6661 actgcgactg cgatcatgat cagcattaaa gaaatgactg taaagtggaa aacatcgaat
     6721 ttctcatcgt tggagacgaa ctctttttga ggatcttttc tgtaaagcat tttgaaaaat
     6781 aagtttccca tatttatgaa aattaaaaga aacatgaaca ttgtaatcag cagaatgtaa
     6841 ggcctttgtg aaaaaagcat cccttcaaag atcttgattt cactgaaaaa gaatccgaaa
     6901 gggggaattc ctgaaacggc aaagaaagag ataaggaaaa gccatcctgt ccaggggagt
     6961 ctttcgagaa ctccggaaat ctcagaaata ttttttgaat tatatttatg gtggatcttc
     7021 cctgcacaga gaaaaagtcc gacctttgtc attgaattaa aaacaatatg gtacatggct
     7081 cctatgaatg caagaccgcc ggtccctatt cccagaatta ttattcccag atgttcaacg
     7141 cttgaatacg cgaccatttt tttcatgtct gtcgctttga gcataaagat cagtgttact
     7201 aaaatcgaaa gaagtcctcc ggtcacaagc agaaatttcg caaattcgta tgagccggta
     7261 ttctttaata tctggagcat ccgcaatact ccaagaagtg cggttattct cagtgttcct
     7321 gaaaaaaggg cggcgatggg gcttggagca gtgcttgtcg catcaacatc tccagcgtgc
     7381 atcggtgcaa cccctatttt tgtactaaga ccgacaaata taaagacaaa cccggcttta
     7441 agccatacag ggctgagcag atgtgcattt ttaataaatt cagaaaaata aagctgctga
     7501 ccgtcaagga cggtgccttt tgatgataat gcaaggaaaa gaattccgat aaaagcaaaa
     7561 gcgatgccga ctgaaactat gaaaagatat ttccacatcg cgataagtga ttcctgactc
     7621 cttgaataat atacaagagg agccacgctc agagttgttg cttcggcggc aacccagtac
     7681 attgcaaaat gattgcttaa aattgccgcg gtatttgcaa taatataaaa gttcatgagc
     7741 atgaaataat attttttgcc gcttgttgaa tccgattcaa ccggtagttt gatataggaa
     7801 tatgaaacaa gtactatcca gaaataagta tttgaaagga caacaagaaa cagcttgctt
     7861 agtgaatcat atccgaacat gaagaattgt tccggaatgt aaaatccggt aaagatcgca
     7921 aatgaaaccg ttaaatgcaa aaaagcagac agcagtgtta cggcatactg tattttccag
     7981 tttttgaaca gaaaagatgt aactgccgcc ataaacggca gtgtaagcat aacaagaatc
     8041 gcagtcatat ctaaggatcc ctcctttgtg ccagcctgtc atctgtgaaa atggtcgagc
     8101 ttattttgtt tatggcaatt cccattaaaa atactactgc aaaaacatca agaagacttc
     8161 ccagctctat tattattgga aactctgatg caaccgcaag tccgaacatg aatattccgt
     8221 tttcgagcac aagaaatccg attacatgaa caatgagctt tttccggaac attattatgt
     8281 aaatacctat agttacagct gaaaatgctg tggcgaaagg tattacggca aattcggtgc
     8341 tttttgagag atagtttgaa acgatgaata tgacagccat ggaaaagcac gaaagaagaa
     8401 ggaagttgaa ctgctgaaga gttgactcga tctctctttt tatgttgagg tcattcataa
     8461 ttttttccat gtatctgggg atcaggatga ccttgacaac aagcattgtt aaaggaagaa
     8521 ttaatccgaa gatcgaaaaa tcaggaataa aagggagtat cattaaaata acaagaagca
     8581 ctccctgcat tctcagaacc gatatgtagg atttgacact cgtagtgaca accaggtaaa
     8641 aaagtgaaag acagaaaagg atgctgagta cattttgcat ttatacacct actccgaaaa
     8701 tgtaaatatc agcaggttta aaatacctat cgccgtagca aaaaggagat attgaggaat
     8761 attcttcatc ctgaatcttg aggtgacagt ctctgtcaat gcaagcacag atgaaatagc
     8821 gaacattact acaagaaata caagaagcgc aagccagtag ttttcaacgc tgaaaggata
     8881 gaagaaagaa gtaatgatca ccgaatatat aaaaagcttg atataacttg agatctgata
     8941 gaagaacaag tctattccgg agttgtcaag tatcatgacc tcgtgtatca ttgtgagctc
     9001 aaggtgggtg ttgggatcat ccacaggcat tcttgaacat tctgtcacag caagaatgaa
     9061 tgatgatatt gaacacacaa cgacgaaaac tattaccgag atgttgttaa gagaaacgga
     9121 atgaaaaatt ccgcttatcg aagtgtgccc gcttataaac gcgatgcttc cgactgtgaa
     9181 aaagaagatc ggttcggcaa atacggcaaa aagggcttca cggcttgccc ccatcccttc
     9241 aaatgagctc ccgatgtcca ttgccgcaag gatctgaaag aatcttgcga gtcctagaag
     9301 atatgcaaaa agtatgatat ccccttcaaa gcttatcaat ggttgccaca gaccgatcgg
     9361 aagtattgcc gcggctactg ttacagaggt aaaattaata aggggtgaaa aaacgctgat
     9421 gaaagatgaa tcccctgcat taatggtttc cttcttaaat aattttatca gttcataata
     9481 gggctgaagc accggcgccc ctttttttcc gataaacaga gctttctgtt tatttacaat
     9541 gccggcaaac agaggggaaa acgcaagaat tagaaaaaag gatatcaacc cctgtactat
     9601 ctcgatcagt tccataaaaa cgccaccatt gtgtaaattg caatagaccc cagtatgaat
     9661 gcaacataat atctgacatc agatctgctg aagtagtcaa attttgcgat tatcgccaca
     9721 gattttctat aaacgggaag cacgatctta gtgtctataa aatcatctga gtgtgaatga
     9781 aatccggtct caacaggaaa aaccgaatca tggtcctgat attttttgca ataaacaagc
     9841 agagctttcg gtattatatt gagttcgtcc gcatacgaag aggcggtgta ttgcattttc
     9901 ggtgtgagaa ggtcataccc gcatccccat gccgctgatc ttctgccgcc gtatttcttt
     9961 ttcaggatcc ttcttaaaac aaaaagaaca gctacgcctg ctgtcaaaga aatgcagaca
    10021 aggttgatat acccccattt tattgaaaaa agagttccct tccctgccgg cagaaaacct
    10081 tcttcaagaa caccgttaat gatccataga accggttgag ggtaaaagcc gatgaaaata
    10141 cagaggatcg caaaaaaacc aagagagatg tattcagcca tggaaacttt gaaggaaacg
    10201 atctttttcc tttccgatcc cagaagcatt attgagttta tttttgtaaa gcaggcaact
    10261 gccagaccgc ctaccagagc aagtcctaca acggacacta tcatcgcaag agaataatcg
    10321 ttctttaatt cgtttgagat catgaagaac ccgttgaata tgataaattc agaaataaag
    10381 ccgttcagtg gagggattcc gcttattgca acgcttccta taatgaaaag aagagtaaaa
    10441 aattttgagt ggtgaactat tcctcccatt gattcaatgt ttcttgtccc gagattctgg
    10501 taaattatcc cgcttcctgt gaaaagaaga cttttaaata ttgcatggtt caatgtgtgg
    10561 agaagcgctc cgccgaatcc cagaacctgt attccgatag aattatttgc cgaaccgata
    10621 agcccgatcc cgatcccgag acctatgatc ccgatgtttt cgatcgaatg gtatgcaaga
    10681 agttttttta agtcatgctg ggcgagggca taccacactc cgaatattgc actgaccata
    10741 cttactgcaa gaacaaacca gcccatccac tcggaaactg acggaaggaa aaggattgtc
    10801 cgcataatgc cgtatatccc aagttttatg ataattcctg aaagaaatgc actcagcggt
    10861 gtcggtgcta cggggtgtgc tctcggaagc cagaaatgaa aagggatgaa tcccgctttt
    10921 atcccgaatg cagcgaagga cagtagaaat attgcggcaa cgatcgaact ttctgagcca
    10981 gacaggtcaa aatcggtaaa aaaccagctt cccgattcat gatgcagaag aaaaaatgcg
    11041 atatacataa taaatacaag aacatgtgat gcaacaaaat aatagaaact ccctttttga
    11101 acatcaggtt tttctttctc aaaaagcatg gcaccataag ctgatgtccc cattatttcc
    11161 cagcttataa gaaacatgat ggcatgattt gatattacaa tgaactgaca ggcgacaaca
    11221 agaatgataa aaaacacgat ggagcttgcc gccgaatgtc cttttttaat gtaatgatca
    11281 aaatatgaga gattatagat ggatgtgggg acggcgatga tctgtatcac aaaaagaaaa
    11341 aactttgtaa gaccgtcaaa acggaacatt ccattatatg atatgttaat tccggcatga
    11401 ccgggaagaa gtgaaatcag aacaggaaga aaagagatga taattccaat tatgagaatt
    11461 acctgtgaca gtatcagtga ctttttacgg tctttgatca atattgcgaa tataagtgcg
    11521 gtcatctgaa ccgcaagtcc cgggaagaac aaactttcca tacctgtagc atcctgcagt
    11581 aaaatgctga cacaaaccgt taaatcgacg gcagtatagc actttgaaat ctattttcaa
    11641 ggaaaaggtt cagcggttga atttcgccat cagatctgcg tatgcttctt caaggttcat
    11701 cccttttgat gaagcttctg caagatattt ttcgagcggt tctctccagt cccttgatgt
    11761 gatctcgtta tttgaatcgt caagaatgaa tttgtagtaa tctccgccgc tgtagatata
    11821 ttcatggatg accgctgtat atttccgttc tttctgaacc ggctcaccct ttatgaacca
    11881 gtcattccct tttttgatag ctccgcagta gctttcctgt gaaagattga gattgtatat
    11941 caggtcttca cctttcaatg ttgaaattac tatccggttc tggaagggaa gcattgagat
    12001 tatatcactc ttcttgaccg ggccgcaatc aatgacatcc ctgaacgctc cggggttgct
    12061 cattgccgca tcatattgag gaaaagaaca aagaagtgag catgcgtaaa gtttctgaaa
    12121 tgattcaaat tcaaaatcct tttcagaagt tataagccgc tgacccgcct ttttatcgag
    12181 cttttcaaga tatgaatcgg ttatgtcttt aattttcttt gatgtatcag atgaaaaatc
    12241 cgctttttta cgaagaggga tcacatcaac ttttgtaacg gtgacagatt ttcccttttt
    12301 ctgaacttcg acccgtgcat aacttttcag atatccttct gtctgaacga aaaggacatc
    12361 attataattc ttaactatcc gaacatgttc gtgcgcaccg aaaacaatga gcggctttac
    12421 aggcagatct ttgacccagt cctttgccgt ttcagcggat tcatgcacaa taactacctg
    12481 aaaatctgaa agcggttcgt tctttttcaa ttctctgccg acggttgaca gcggttttac
    12541 aactctcaaa tttttcaggt agcttgcaaa agagatctgc ttgagttctt ctgtagtagc
    12601 accgataaaa gagatcctgc cgtctttaaa tggatagatc agagatggct caaaagcgtt
    12661 tttaaggtcg gacgaaataa tgttggaaga aaggtattcg atgcctgaaa tcttcctgtt
    12721 attctcaaaa gggagtgttc cgaaatcaaa ttcatggttc cccagagcac ttattctgta
    12781 acctgtaagc cccattattt tggcaaccgg ctctccgttg aataaagatg atatcgctgt
    12841 tccggtgtaa ttatcgccac ctgaaattct gaagacatta catcccctgc agttcttttc
    12901 ctcttcctcc cacatttcat aaagaactga acctttatac cagccgtcct tttcataaat
    12961 gtgaccgtgc tcatcgtttg tata
//
LOCUS       DAOOYB010000064        12845 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_5023_length_12845_cov_12.969977, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000064 DAOOYB010000000
VERSION     DAOOYB010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12845
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5023_length_12845_cov_12.969977"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..351
                     /locus_tag="PL056_05405"
     CDS             121..351
                     /locus_tag="PL056_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017732857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46485.1"
                     /translation="MDRLLLIKKTNGYLKKLPSEKLIEVHDFVEYLYSKCDQYVLREG
                     MSYLSSKSGSLDFLNDEEEDVYTVSDVKEEYK"
     gene            348..668
                     /locus_tag="PL056_05410"
     CDS             348..668
                     /locus_tag="PL056_05410"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HPM46486.1"
                     /translation="MKGDIVLVNFPFSDMTGNKVRPACVLADDGRDVTLAFISSVIGN
                     SGIFDIKLKPNSTNNLKKESLLKVSKLATLSRKIILGKIGVIEQTEINDVDKLLVHYF
                     QIKC"
     gene            720..1484
                     /locus_tag="PL056_05415"
     CDS             720..1484
                     /locus_tag="PL056_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46487.1"
                     /translation="MKKRLIILIVFLLFLLSCGGVEETDNDSDETEDIETSDSDTFDG
                     SEVIMFKDVNLEKCVRTKIQKPEGEITVEDVKNVKSLDCLRMNIKVLDGIEKLPSLSN
                     ISFYENDIKDFSSLKGINTLKKLNIFRNENVDINTLSELNSLEELDVSSCGLTTLEPL
                     KNLEFLKVLSANTNNLTSIEEVKNLTGLESLILNKNYLQNDDLPVLLNLVNLEHLNIR
                     ENCITDFTVIDQMKENLPDAEILGGIIEAQDTTRCK"
     gene            1513..1806
                     /locus_tag="PL056_05420"
     CDS             1513..1806
                     /locus_tag="PL056_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46488.1"
                     /translation="MKKKFLLILLMLFVASCGGVEETDNDSDEMTDMESNDDENSIEF
                     PWTDPHTGLVWSVKNDRYGVKHDSANYFCKNMKENEKTQNLKISRNSLIFVTV"
     gene            2032..3354
                     /locus_tag="PL056_05425"
     CDS             2032..3354
                     /locus_tag="PL056_05425"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HPM46489.1"
                     /translation="MKNYFYKLADLAVSKTKAGEFIYLNLSGEESDFIRLNHALIRQN
                     GHVSQYYLSITLIFGGKQAHHSLSLSKNFETDSMSVDNALNSLRNIVSQVEIDPYILY
                     STEVNSTETVLKNELPESENVVNEIIALAKGLDLVGIFAGGNIIAGFANSAGQKNWFE
                     KPGFNFSWSVYHSTDKAVKSGYAGYKWDESRFNAKMEKVRTELEIMKRTPVTIQRGKY
                     RVYLAPSALEDFFGVIGWGGFGIKSQKTKNSSLIKLVENEVTLNEAVTVTENTLEGSS
                     PNFNSTGFIKPDVVKLIEKGKHAGSLVSPRSSKEYGEIQNGANGYEMPESLEMNGGNI
                     VATDILKVLDTGIYLNNVHYLNYSDRASCKVTGMSRFAAFYVKNGEITAPINVMRFDE
                     SVFKMFGENFVDATSEREMLISTDTYGQRSTESSHLPGIIVKDFSFTL"
     gene            3481..4074
                     /locus_tag="PL056_05430"
     CDS             3481..4074
                     /locus_tag="PL056_05430"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPM46490.1"
                     /translation="MICGKRIREIRIILKKNQKLMAEELGISQSYLCAIERGAKRPNG
                     NTIIKLVNNFHVSCSWLMDGTGPEFLDCNDICYIRSAEVNEKAPLYNFPVLKQYLQDT
                     VGINADMLNFYIADSDNMEPAIKKNDIVLVDTSENLCDMEGVYLFRIESRKFLGRLLF
                     FPQKHIVNDNPTIKNSSRIFDSSVECVGKIVWFCRKL"
     gene            4150..4374
                     /locus_tag="PL056_05435"
     CDS             4150..4374
                     /locus_tag="PL056_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013749205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HPM46491.1"
                     /translation="MFLSDLKSGEAGVVVSIESGLRLFNRLASMGISEGRELKVVKNS
                     GEGPVVIDVEGSRYAIGRGMAQKIVVSEKL"
     gene            4371..6404
                     /gene="feoB"
                     /locus_tag="PL056_05440"
     CDS             4371..6404
                     /gene="feoB"
                     /locus_tag="PL056_05440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HPM46492.1"
                     /translation="MKSIRIAIAGNPNVGKSTLFNKIAGSDQHVGNYPGVTVERKEGR
                     VSFKDYELTVIDLPGIYSMNAFSPEEIVTRNYIAEERPDVIINIVDASNLEKNLYLTL
                     QILEMKVPVVLFLNMLDSAEVKGIGISREILEKEVETPVIEGIAKKGTGINEVLEKCI
                     EIYEKKPPLAVVPYSEMVGRYISRIKKAIELESPEIAKDWRAIRFIEGSLVERKYHSS
                     EVLDKIHEIIQDLMNETGHRIHSMVMVQERYHLIKNIVNASMCVSCVQKEDVTDKIDK
                     LVLHRIWSMPIFLFMLFSLFQVVFTLGEPIENLLALVFDQLGNFAASLWPQGADSLLK
                     NLIVEGIIGGVGGILVFTPYIFLVFMTISLLEDSGYMSRVAVIMDKWMGKIGLSGKSF
                     MPMILGFGCTVPAVMGARIIENKFERLATIMVIPFMSCGARLPVYLLIISAFVPLKFQ
                     AVVLLLVYATGVFFAAVSAKILRLTIFKGEENPLIIELPKYSTPSLKSVWMLTWHRGK
                     HFLEKAGTIILFASIVLWVLNTFPRIDTEELTGMSENDAALLKAENSFSGRIGKAIEP
                     AFKIMDADWKVASSFLASLAAKELFVSQMSILHAMGNEDENSAALQNKFKEGYSLATA
                     IAIILFILLSAPCVATFAVVKVETDSWFWPTVQYAGMIIIAYFAALAGFFIFK"
     gene            complement(6453..6893)
                     /locus_tag="PL056_05445"
     CDS             complement(6453..6893)
                     /locus_tag="PL056_05445"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HPM46493.1"
                     /translation="MKIVRYREPNRLVPGSFNNWMEDLFSEFFNNNLERIPAASIYPK
                     VDIEENENSYVFKAELPGVRKEDVSIEIDNRVLTISGEKKEEREEKSGNVYRKESYSG
                     SFSRSFTLPEHVNPDDADAKYENGILFLSIPKSEDKAKKKIDIK"
     gene            complement(7032..7910)
                     /locus_tag="PL056_05450"
     CDS             complement(7032..7910)
                     /locus_tag="PL056_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46494.1"
                     /translation="MSVGKFLLNKENLNFVAKNSFKFSKSYIVDETMRYFEKNEKEVQ
                     KIAENLEYMGLSSTGEGLDHALKGIAVHYFEKLFVLTKNYEMFWIAKNKIETGSSLEI
                     IKEAQDSKKAVFAAQSHFGASYFMSITFMVNGFNIASVGKFPEPVGSMIKNGSETIAQ
                     KYGTGKTKLINIADPDCDVAYEMFSTLLRGGILSNVFDENNQFSNEVKLLGKKVMGGS
                     GMDIILQKWNDEKLIVVTPFIIRTGDDTYRLELDRHYLNKGNIIQSFYDSLGKRVATH
                     PDQWYFIHELHEAIPE"
     gene            complement(7913..10279)
                     /locus_tag="PL056_05455"
     CDS             complement(7913..10279)
                     /locus_tag="PL056_05455"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPM46495.1"
                     /translation="MKYDITIIGSGIGGLTCALELARNGYSVCVIEKHNQPGGYAHSF
                     KRAGFEFDVSLHHIGGLSKGKSIHGVLKTLGILDKIGFTKKKNILSVRFPDGEKTVPN
                     RPGAFENFLINEFPQEKENIIALISHLKDLRWHIIGGWIDPDFDVPMEKLLTKDYLDK
                     TFHELLTKFVHDEKLIAYLSQFWILIGLPPKLVNATFATCVFNSHFLEETYDIDGGGA
                     ALSKAFVSRMEELGSKVMLNSEVSKITVENGRATGVVLNNGTVINSEIVISNVDPYQT
                     FTKLVAKEFTSDLFRFRLEKMEKSVSLYSLYLGLDCKPSELGVPDTTFFYNHSYEPLK
                     GYANILKGNSESTDWCCTNYENSLINKAPKGNYVLTFVEVTPTADWLTLDKESYKIRK
                     QEILNTLLNKYNKCFPGLKDHIKVMEFATPRTMNRYTSNCGGSVYGLAQTVEQSASKR
                     LRNITPVENLFLTGAWTWSGGGYEGAIMSGIQTSASIKKKWGSKNDALPIKVKMDIGK
                     PSSRTYNKFVRIYNKDVSAGGIADMNCFLRLMDRGRVDSGEELFNIAETESLFTHYNI
                     QVYSITVKKHRDNFSGDKLNVTSRYFRRTSIRMVCHQQLINSVTGKETIDAIVELVQL
                     DDNGKLIDIPAEINDPTDPPVICDISKYAVSLKKKSSHFFHKIRVYTEDTDLQGVTFH
                     ASYLRFCEETLFEFLDKVCRSTKNFTHWVYPEFNIRFFNSARVGEILDINLTADLTSD
                     GRLVFFETMGIEGTDKIGVQICFDLELRNDKNEKVPFPAELREFIGRG"
     gene            complement(10279..>12845)
                     /locus_tag="PL056_05460"
     CDS             complement(10279..>12845)
                     /locus_tag="PL056_05460"
                     /inference="COORDINATES: protein motif:HMM:NF020243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPM46496.1"
                     /translation="PEDARPKAPVRTITGLAKAFSEASCRGETPFSHSPASPASQTSQ
                     TSQNSTESEKKNEFDIELDSEKPSEWSLFTRNWEFVRPDDLKKFNCENVLVIAEKKEH
                     ASDLKNGLKKLGIDCSIKIPADFLKKDPEKSQKLLRDCDTIVYLAHYDLVSAERNNEN
                     DKVFYQTTKELFELFKMFYGTISENSKRVIVPVAKNSAFESVPLNGQFYSAFPTGFFR
                     SMKNELPGSQVMIINSDPENWNGTIIDNINVCFSGFDMGFINNSPVKGALSKVINSKK
                     LDYQLDKDDSVLVTGGARGIVFECVAALSAKTGCRLILTGRTTKPGSEIEGLSFDRDN
                     MTASIRELEMKLVKERKLSLKDAKKEALSLKSAWEVSENLRRLSDSGINARYEKCDVS
                     NPEELKKLVSKLKKENVVITGVVHGAGVQRSKMLPDLEMDDIIRTIETKINPLFAMLE
                     ILDWKKIRFFNAFTSIAGLFGNAGQTDYAFANDILAEMTVSIKVRYPHIQVSSIDWTA
                     WSGTGMVSKEEAKRFKESGLIPVDIENGVKMFLKAAGDPTHIRFAVFNEGAAFAGDAV
                     TKYSLSSNPFRTLSETHSEPMKVNFSLKRDIYLHQHVVNGAPVVPGTFVSEIFAENLL
                     SKGSSISGLKFRRPLAVNSDHFNVELVKSGDRTFIVPLDRPVLEGKGLENLSFSSCDT
                     KTSSKPKKPDWINFDIDEIDKKFRSKSEKNYFYQYLDEKFSHALKTGPIFRGIVSTFE
                     EDGIYYSTVALTKDAVASFAIPGEFKFNPVLADMAVQVASAWNMKKQDVMAIPAEIES
                     FNVFEKTSSTDSVVVCRMVDMTPAQATFDVAVFERDGKLIFTMEKLLLKTIAGLEK"
BASE COUNT         3791 a         2374 c         2614 g         4066 t
ORIGIN      
        1 gctcctcctc ttcagttaga aatctgctga attttttttg ggggaacttc aattgatacg
       61 actgcttgac agaaaaacgg gacaagttat aattataaga atttttttac ggagattcaa
      121 atggacaggc ttttactgat taaaaaaacg aatggttatt tgaagaaact cccaagcgaa
      181 aaacttatag aagtgcatga ttttgttgaa tatctttatt caaaatgcga tcaatatgtt
      241 ttaagggaag gaatgtcata tctttcttca aaatcaggaa gccttgattt tctcaacgat
      301 gaagaagaag atgtttatac tgtcagcgat gtgaaggaag aatacaaatg aaaggagata
      361 ttgttcttgt caatttccct ttttctgaca tgacaggaaa taaggttaga cctgcttgtg
      421 ttcttgctga tgatggccgc gatgtgaccc tcgcatttat atcaagcgtg attggaaatt
      481 ccggaatatt tgatattaaa ctgaaaccaa actccacgaa taatctaaaa aaggaatctc
      541 ttttaaaagt gtcaaaactt gcaacgctca gtcgcaaaat aatattggga aaaatcggtg
      601 ttattgaaca aacagaaata aacgatgttg acaagctcct tgtccactat ttccagataa
      661 aatgttaaaa tttttgtcta aattgttttt ttgcgagaag cttaaatagg aggatgcgca
      721 tgaaaaaaag actgatcatc ctgattgtgt ttctgctttt tcttttatcc tgcggaggag
      781 tagaagagac agataatgac agtgatgaaa cagaagacat tgaaaccagt gattcagaca
      841 cttttgacgg ttcagaagtg ataatgttca aagatgttaa tcttgagaaa tgtgtgagga
      901 caaaaataca gaaaccggaa ggggaaataa cagttgaaga tgttaagaat gttaaatctc
      961 ttgattgtct cagaatgaac ataaaggttc ttgatggaat agaaaaactg ccaagtctaa
     1021 gcaatatttc tttttatgaa aacgacatca aagatttttc atctctgaaa ggaataaaca
     1081 cccttaaaaa actcaatata tttaggaatg aaaatgttga tattaatact ttgagtgaat
     1141 tgaatagcct tgaagaactg gatgtgtctt cttgtggctt gacaacactt gagccgttaa
     1201 aaaatctaga gtttcttaaa gttttatctg caaacacaaa caacctgacc tcaatagagg
     1261 aggtaaagaa tttaactggt ctggaatctc tcattttaaa taaaaactac cttcaaaacg
     1321 acgatcttcc agtattgctg aatcttgtga atcttgaaca tttgaatatc cgggaaaact
     1381 gtataaccga ttttacagtc atagatcaga tgaaggaaaa tctgccggat gccgaaatac
     1441 tcggcggaat aatagaggca caggatacca cccggtgtaa atagaaaagg agcattatag
     1501 gtgcagttaa acatgaagaa gaagtttttg ttaattctgc tgatgctgtt tgtggcttcc
     1561 tgcggaggag tagaagagac agataatgac agtgatgaga tgacagacat ggaaagcaat
     1621 gatgatgaaa acagtattga gtttccctgg acagatcctc ataccggtct ggtctggtcg
     1681 gtcaaaaatg atagatatgg agtaaaacat gattctgcaa attatttttg caaaaatatg
     1741 aaagaaaatg aaaaaacaca aaacctcaaa atttcccgaa atagcttaat atttgtcact
     1801 gtgtgacggt gcggtcaggt gttgaaaaat tcagtgcgtt gcattaaaaa acgacctcga
     1861 ttattattct ttttacactt ttctggagat aaaaagcagt aaaatttaca cttttttgga
     1921 gatagcatct gaaaacttta gaagtaaatt gccaaagcgt ttgacttgtt ttacaatcgt
     1981 gtgattttct ggagaaagtc gctgtttgtc aatttgagca ggaggtgaat aatgaaaaac
     2041 tatttttata aacttgcaga tcttgcagtt tcaaaaacaa aagcgggtga atttatttat
     2101 cttaatttaa gcggtgaaga gagcgatttt atccgtctaa accatgcatt gatcaggcag
     2161 aacggtcatg tttcacagta ttatctttca attactctca ttttcggagg gaaacaggcg
     2221 catcactctc tgtcgctgtc aaaaaacttt gaaacagatt cgatgagcgt tgacaatgct
     2281 ctaaattcat taagaaatat agtttcacaa gttgagattg atccttatat tctctattca
     2341 actgaagtga attcgaccga aacggtttta aaaaatgaac tgcctgaaag tgagaatgta
     2401 gttaatgaaa taatcgcttt ggcaaaaggt cttgatctgg tggggatatt tgcaggcgga
     2461 aacatcattg caggttttgc aaacagtgct ggtcagaaaa actggtttga aaagccgggt
     2521 tttaatttca gctggagcgt atatcattca acagataaag cagtgaaatc gggttatgcc
     2581 ggctataaat gggatgaaag caggtttaat gcaaaaatgg agaaagtgag aacagagctt
     2641 gaaataatga agcggactcc ggtcacgatt caaagaggta aatacagggt ttaccttgct
     2701 ccaagtgctc ttgaggattt tttcggagtg atcggctggg gcggtttcgg aattaaatca
     2761 caaaagacaa aaaacagttc gttaatcaaa cttgttgaaa atgaagttac tttaaacgaa
     2821 gcggtgacag ttactgaaaa tacattagag ggaagcagcc caaactttaa ttcaaccggt
     2881 ttcattaagc ccgatgttgt taaacttatc gaaaaaggaa aacatgcggg atcgcttgta
     2941 agcccgagaa gttcaaagga atacggcgaa attcaaaacg gggccaacgg ttatgaaatg
     3001 cccgaatctc ttgaaatgaa tggcggaaac attgtggcaa ccgacattct aaaagtgctt
     3061 gatacgggga tttatcttaa taatgtccac tacttaaact attcagacag ggcaagctgc
     3121 aaagtaacag gaatgtcgag atttgccgcg ttttatgtga aaaacggtga aataactgct
     3181 ccgatcaatg tcatgagatt tgatgaatcg gtatttaaaa tgttcggcga aaattttgtt
     3241 gacgccacca gtgaaaggga aatgctcatc agcacagata cttatggcca gagaagcacg
     3301 gaaagttcac atcttccggg gatcatagtg aaagattttt catttacgct ctgaaattaa
     3361 atatttttct cttgattttt tcatttctaa gtgttagttt cgcgtgttaa tgttaattaa
     3421 aactgcaaga acagattata tacaaataag aatgttgttt atatcctaca ggagagtatg
     3481 atgatttgtg gaaaaaggat cagggaaatt cgcattattc ttaaaaagaa tcagaagtta
     3541 atggcagaag aactcggaat ttcacagtct tatctttgcg cgattgaaag gggtgctaag
     3601 cggcccaacg ggaacacgat aataaaactt gtaaacaact tccatgtaag ctgttcgtgg
     3661 ttgatggatg gaaccggacc tgaatttttg gactgtaatg atatctgcta cattagatct
     3721 gcagaggtca atgagaaagc accgttatac aattttcctg ttttgaaaca atatcttcag
     3781 gatactgtcg gcattaatgc agatatgctt aacttttaca tagcggattc tgacaacatg
     3841 gagcccgcga taaaaaagaa tgatatcgtt cttgttgata cttctgagaa tttatgtgat
     3901 atggaaggtg tgtatctttt cagaattgaa tcgagaaagt ttttaggcag acttctcttt
     3961 tttccacaga aacatattgt aaatgacaat cctacaataa aaaacagcag cagaatattt
     4021 gattcatctg tagaatgtgt cggcaaaata gtctggttct gcagaaaact gtgaattttt
     4081 ttatactatc ttattgacaa attagccaaa gctaactata ttccccatag ttaatttatg
     4141 gggtttgtca tgtttctttc tgatctgaaa agtggtgagg ccggtgttgt ggtatctatt
     4201 gaatccggtc ttcgtctttt taaccgactt gcttcaatgg gtattagtga agggagggag
     4261 ttgaaggtgg ttaaaaacag cggtgaaggt ccggttgtca ttgatgtcga agggagtaga
     4321 tacgctattg gcagaggtat ggctcagaaa atagttgtat ctgaaaaatt atgaaatcta
     4381 ttagaatagc tatagcagga aatcccaatg tggggaaaag tacacttttc aataaaatag
     4441 cgggaagcga ccagcatgta ggcaactacc ccggtgttac agttgaaagg aaggagggta
     4501 gagtcagttt caaagattat gagctgacgg ttatagatct gcccggaata tactcaatga
     4561 acgctttttc tccggaagag atcgttacca gaaattatat tgcggaagaa agacctgatg
     4621 ttataattaa tattgttgat gcttcaaatc ttgaaaagaa tctgtatctt acactgcaga
     4681 tattggaaat gaaagtcccg gttgtccttt ttttgaacat gcttgattcg gctgaagtga
     4741 aggggatagg tatttcaagg gagatccttg aaaaagaggt cgagacaccg gttattgaag
     4801 gaatagcaaa aaaagggacc ggaataaacg aagttcttga aaaatgtatt gaaatatatg
     4861 agaaaaaacc tcctctggct gttgttcctt attctgaaat ggttggcagg tatatttcaa
     4921 ggataaagaa agctattgag ctggaatcac cggaaattgc aaaagactgg agagcgatac
     4981 ggtttattga aggaagtctt gtagaaagaa aatatcacag cagtgaagtc cttgataaaa
     5041 ttcatgaaat aatacaggat cttatgaatg aaacaggtca caggatccat tccatggtca
     5101 tggttcagga aagataccat ctaattaaaa atatcgttaa tgcctcaatg tgtgtgtctt
     5161 gcgttcaaaa agaagatgtt accgataaga ttgacaagct ggttcttcat cgcatctggt
     5221 cgatgcctat atttttattt atgctgtttt cactttttca agttgttttc acacttggag
     5281 agccgattga aaacttgttg gcgttagttt tcgatcaact gggcaacttc gccgcatcgc
     5341 tatggccgca gggagctgat tcgcttttaa agaatcttat tgttgaagga attatcggcg
     5401 gagtcggagg gatactggtc tttacgccgt atatatttct tgtttttatg acaatatcgc
     5461 tgcttgaaga ttcaggttat atgtccaggg ttgctgtaat aatggataag tggatgggca
     5521 agataggttt aagcggtaaa agtttcatgc cgatgatact tggcttcgga tgcactgttc
     5581 cggctgttat gggtgccagg ataattgaga ataaatttga acggcttgcg acaataatgg
     5641 ttatcccttt tatgagttgc ggagcacggc ttccagtcta tcttttaata atatcagctt
     5701 ttgttccttt gaaatttcag gcggtagtat tgcttcttgt atatgcgacc ggtgtttttt
     5761 ttgcggcggt cagtgcaaaa attctccggc ttacaatttt taaaggtgaa gaaaatcctt
     5821 taataattga actgcccaag tattcgacac catctttaaa atcggtctgg atgcttactt
     5881 ggcacagggg aaaacacttt cttgaaaaag cgggaacaat aattcttttt gcgagcattg
     5941 ttctttgggt gctgaacact tttccgcgca ttgatactga ggaactgacg ggtatgagtg
     6001 aaaatgatgc agctctgtta aaagcggaaa actctttttc aggcagaatc ggaaaagcga
     6061 tagagccggc atttaagata atggacgctg attggaaagt tgcgagttca ttccttgcaa
     6121 gtctggcagc aaaagagctg tttgtttctc agatgtcgat acttcacgca atgggaaatg
     6181 aagatgaaaa ttcagctgca ttgcagaata agttcaaaga gggatattcc cttgctacag
     6241 ccatagcgat cattcttttt attcttcttt ccgctccttg cgttgcaaca tttgcagtcg
     6301 taaaagttga gacagattca tggttctggc cgacagttca gtatgccgga atgatcataa
     6361 ttgcctattt tgccgcctta gccggatttt tcatttttaa ataaaaaacc cggccaattt
     6421 gaccgggttc atgaactgga ttttcactga tgtcatttga tgtcgatctt cttttttgcc
     6481 ttgtcttcac ttttcggaat tgaaaggaaa aggatgccgt tttcatattt tgcatcggca
     6541 tcgtcaggat ttacatgttc aggcagagtg aacgaccttg aaaatgagcc gctgtaagac
     6601 tctttccgat aaacatttcc gcttttttcc tctctttcct ctttcttttc cccgcttatt
     6661 gtaagaaccc tgttgtcgat ctcaatggaa acatcctctt ttctcacacc gggaagttcg
     6721 gctttgaaaa cataggagtt ttcattttcc tcgatgtcaa ctttcggata gatcgaagca
     6781 gcgggaatcc tttccagatt gttgttgaag aactcactga aaagatcttc catccagttg
     6841 ttaaatgatc cgggaactaa tctgttcggt tccctgtaac gaacgatctt cataattaac
     6901 ctccatttca aaagttaata aagcccagta ataacaaggt gttaaagagc tttttcgctc
     6961 acggattttt ttatatgcac gattttttat gagtcaagag gggagatatt gaaaaaatta
     7021 tttcaaacag atcactcagg tattgcttcg tgaagttcgt ggatgaaata ccactgatca
     7081 ggatgtgtag ccaccctttt tccgagtgaa tcataaaaac tctgaatgat gttccctttg
     7141 ttcagataat gtctgtcaag ttcaagccgg tatgtgtcgt cgccggtcct tataatgaaa
     7201 ggtgttacaa cgatcagctt ttcatcattc catttctgca gaattatgtc catccctgat
     7261 ccgcccatca cttttttgcc taacaatttg acttcattgg agaattgatt gttctcgtca
     7321 aacacattcg aaagtattcc gcctcttaaa agagtgctga acatttcata agccacatca
     7381 cagtcaggat ctgcaatgtt tatcagtttt gtttttcctg ttccgtattt ttgggctatt
     7441 gtttccgaac cgttctttat cattgaaccc acaggttccg ggaattttcc gacagaagcg
     7501 atgttaaaac cgttcaccat gaatgttatg ctcatgaaat acgatgcacc gaaatggctc
     7561 tgggctgcaa atactgcttt tttcgaatcc tgtgcttctt ttataatttc aagactggag
     7621 ccggtctcta ttttgttttt agctatccag aacatttcat aattttttgt cagaacgaaa
     7681 agtttttcaa aataatggac agcaatccct tttagagcat ggtcgagacc ttctccagtt
     7741 gacgaaagcc ccatatattc aagattttct gcgattttct gaacttcttt ctcgttcttt
     7801 tcaaaatatc tcattgtctc atcaacaatg taggatttac tgaatttaaa agaatttttt
     7861 gccacaaaat taagattttc cttgtttaaa agaaattttc ctacgctcaa tttcatcctc
     7921 tccctataaa ttctctaagt tcagcaggga agggcacttt ttcattttta tcgttcctga
     7981 gctcaagatc aaaacagatc tggacaccta ttttgtccgt cccttcgata cccattgtct
     8041 caaaaaatac aagccgtccg tcagaggtca ggtcagcagt aagatttata tcaagaattt
     8101 ctccaactct tgctgaatta aaaaaccgaa tgttgaattc aggatagacc cagtgggtaa
     8161 aattctttgt agacctgcag accttgtcaa gaaattcaaa aagtgtttct tcacaaaatc
     8221 ttaaataact tgcatggaat gtaacgccct gaaggtcggt atcttcagtg tagaccctta
     8281 ttttatgaaa aaaatgagaa gattttttct tcagagagac ggcatatttt gatatgtcgc
     8341 aaattacagg gggatcagta ggatcgttta tttcagcagg aatatcaatg agttttccat
     8401 tgtcatcgag ctgaaccagc tcaactatgg catcgatcgt ctcttttccg gtcacgctgt
     8461 tgatcagttg ctgatgacag accattctga tagaagttct tctgaagtac ctgcttgtaa
     8521 cattgagttt atcgcctgag aaattatcac ggtgtttttt tacagttatt gaatagacct
     8581 gaatgttgta atgggtgaaa agcgattcag tttcagctat attgaaaagc tcttcaccgc
     8641 tatcgacccg gcctctgtcc ataagtcgca aaaagcagtt catgtctgca attccgccgg
     8701 cacttacatc tttgttgtat atccttacaa acttgttgta tgttcttgat gaaggttttc
     8761 ctatatccat cttgactttt atcggaaggg catcattttt tgaaccccat tttttcttga
     8821 tcgaagctga tgtctgaatg cctgacataa ttgctccttc atagccgccg cctgaccatg
     8881 tccatgcacc ggtaagaaaa agattttcaa ccggtgttat gtttctaagc cgcttgcttg
     8941 cggattgttc aactgtctgt gccagtccgt atacgcttcc tccgcaattt gaagtgtaac
     9001 ggttcattgt tctgggagtt gcgaattcca ttacttttat atgatcctta agtccgggga
     9061 agcatttgtt gtacttattt aaaagagtgt tgagaatttc ctgctttctg atcttatagg
     9121 attctttatc cagtgtaagc cagtctgccg ttggtgtcac ttcaacaaaa gtcaacacat
     9181 aatttccttt tggagcttta tttatcaggg agttttcata atttgtacaa caccagtcag
     9241 tagattcgct gtttccttta agaatgtttg cataaccttt aagcggttca taagaatggt
     9301 tgtaaaaaaa tgttgtatcc ggaaccccca gctctgaggg tttgcagtca agtcctagat
     9361 atagactgta aagagatacg gatttttcca ttttttcaag acggaatcta aaaaggtcgc
     9421 ttgtgaattc ttttgcaaca agtttagtaa aagtctgata aggatcaaca tttgatatga
     9481 ctatttcgct gttaattact gttccgttgt taagtacgac gccggtcgcc cttccgttct
     9541 caacggttat ttttgaaact tctgagttga gcatgacttt tgagcccagt tcctccattc
     9601 ttgaaacaaa tgcttttgac agtgccgctc cacctccgtc aatatcatat gtctcttcaa
     9661 gaaaatgact gttgaataca catgtggcaa aagtggcatt gacaagtttg ggaggaaggc
     9721 ctatcagtat ccagaactga ctgagatatg ctatgagctt ttcatcatgc acaaattttg
     9781 taagcagttc atgaaatgtt ttgtcgagat aatcttttgt aagaagcttt tccatcggaa
     9841 cgtcaaaatc gggatcgatc catccgccga taatatgcca ccgcaagtct ttaaggtggg
     9901 agatcaatgc tataatgttt tccttttcct gaggaaattc attgatgaga aaattctcaa
     9961 atgctccggg tctattcgga acagtctttt ctccgtccgg aaaccggaca cttaagatgt
    10021 tttttttctt agtgaaaccg atcttatcaa gaattccgag agttttaagt accccgtgta
    10081 ttgatttgcc tttagacaat cctccgatat ggtgtaaaga gacatcaaac tcaaaccctg
    10141 ctcttttaaa gctgtgagca tagccaccgg gctgattgtg tttttcaatt acacagactg
    10201 aatatccgtt ccttgcaagt tcaagagcac aggtaagtcc gccgatcccc gaccctataa
    10261 tagttatatc gtatttcatt atttctccaa tcccgcaata gttttaagaa gaagcttttc
    10321 catagtgaat atcagctttc cgtccctttc aaatacagcg acatcaaagg ttgcctgagc
    10381 cggtgtcatg tcaaccattc tgcagacgac gacagaatca gtagaagatg ttttttcaaa
    10441 aacattgaaa gattctatct ctgccggaat ggccatgaca tcctgctttt tcatgttcca
    10501 ggcacttgca acctgaaccg ccatatcggc aagtacgggg ttgaatttaa attcccccgg
    10561 gattgcaaat gaagctactg catctttcgt gagcgccact gtactgtaat atattccgtc
    10621 ttcttcaaat gtcgaaacta ttcccctgaa gataggccct gttttcaatg catgagagaa
    10681 tttttcatca agatactgat aaaagtagtt cttttcgctt ttagatctga attttttatc
    10741 tatttcgtca atgtcgaaat ttatccagtc cggttttttc ggttttgatg aagtttttgt
    10801 gtcacaagaa gaaaaactga gattttccaa tcctttccct tcaagtaccg gtctgtcaag
    10861 cggaactatg aatgtccggt ctccgctttt tacaagttcg acattgaagt gatcactgtt
    10921 aaccgcaaga ggtcgtctga attttagacc gctaatcgaa cttccttttg aaaggaggtt
    10981 ctctgcaaat atttcagaaa cgaatgtccc gggaacgact ggagcaccgt taactacatg
    11041 ctgatgaagg tagatatctc tttttaagga gaaattcact ttcatcggtt cggaatgagt
    11101 ttcagaaaga gtcctaaacg ggtttgacga aagcgaatat ttagttacgg catctcctgc
    11161 aaaagcagct ccttcattga aaacggcaaa tctgatatga gtcggatctc cagccgcctt
    11221 taaaaacatt tttacaccgt tttcgatgtc aaccgggata agccctgact ctttaaatct
    11281 tttcgcctcc tcttttgata ccatgcctgt tccgctccag gccgtccagt caattgatga
    11341 aacctggata tgcgggtatc gaacctttat tgaaacggtc atttcggcaa gaatatcgtt
    11401 tgcaaaagcg taatcagtct gtccggcatt tccgaaaagt ccggcgatcg aagtgaatgc
    11461 gttgaaaaac ctgatctttt tccagtcaag gatctcaagc attgcaaaaa gagggtttat
    11521 ttttgtttca atcgttctga ttatatcatc catttcaaga tcgggaagca ttttcgatct
    11581 ctgtacaccg gcgccgtgaa ctactccggt tattacaaca ttttcctttt tcagcttcga
    11641 tacaagcttt tttaattctt cagggttgga aacatcacac ttttcgtacc gtgcgtttat
    11701 accgctgtca ctaagtcttc tgagattttc actaacttcc cacgcacttt ttaaggacaa
    11761 cgcctctttt tttgcatctt tcaaagagag ttttctttcc ttcacaagtt tcatttccag
    11821 ttctcttatc gaagccgtca tattgtcacg gtcaaaagac aatccttcta tttctgagcc
    11881 cggtttagtc gttcttcctg taagaataag ccggcacccc gtttttgctg ataacgccgc
    11941 aacgcattca aaaacgattc ctcttgcacc acccgttaca agaactgaat catccttgtc
    12001 gagctgataa tcgagctttt ttgagttaat tacttttgac agagcacctt ttacaggcga
    12061 gttgtttatg aatcccatat caaatcccga aaaacagaca ttgatgttat caataattgt
    12121 tccgttccag ttttcaggat ctgaattgat tatcattacc tgcgaacccg gaagttcgtt
    12181 tttcatgctt ctgaaaaatc ctgtcgggaa agccgaataa aattgaccat ttaatggaac
    12241 actttcaaaa gcgctgtttt ttgcgacagg gacaatgacc cttttggaat tttcagaaat
    12301 tgttccgtaa aacatcttaa aaagctcaaa aagctctttt gtcgtctgat agaagacctt
    12361 gtcattttca ttgtttcttt ctgcgcttac cagatcgtaa tgggccagat aaacaatagt
    12421 atcacaatct ctcagaagtt tttgtgattt ttccggatct tttttcagaa aatcggcggg
    12481 tattttaata gaacagtcaa ttccaagttt ttttaatccg ttctttaaat ctgacgcatg
    12541 ttcttttttt tcagcaatga caagaacatt ttcacaattg aattttttca gatcatccgg
    12601 acggacaaat tcccagtttc ttgtaaaaag agaccactca gaaggttttt ctgaatctaa
    12661 ttcgatatca aattcgtttt ttttttctga ctcagtagag ttctgactgg tctgactggt
    12721 ctgacttgca ggactcgctg gactgtgcga aaacggtgtt tcgcccctac aggaagcttc
    12781 actgaaagct tttgcaagtc ctgtgatggt tctgacagga gctttcggcc ttgcgtcttc
    12841 aggta
//
LOCUS       DAOOYB010000065        12635 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_5163_length_12635_cov_8.935692, whole genome shotgun sequence.
ACCESSION   DAOOYB010000065 DAOOYB010000000
VERSION     DAOOYB010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12635
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5163_length_12635_cov_8.935692"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1667
                     /locus_tag="PL056_05465"
     CDS             <1..1667
                     /locus_tag="PL056_05465"
                     /inference="COORDINATES: protein motif:HMM:NF039815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46497.1"
                     /translation="SGDSGNSGNSGDSGNTGDTGNSGNTGNSGNSGNSGDTGNTGDSG
                     DSTVECPCGADDADDDGDGIPNGVEGCNDADGDGLPECLDIDSDGDGISDSEECPSQP
                     CKNSDKDDVPDFMDRDSDNDGLSDKKEKEGGTNPYDKDSDGDGSDDLAEIAYGSDPLN
                     DADSIPAGIFYVVLPYNAPEDVTRTLTFSTKIEAIDVLIVFDRSASMDPEADNLKKEI
                     KTGIIDAVAAQFTSPGFAAYGLTSFGYETMYKMHQTMTLSTDEIKSAVDGLDADEGNE
                     MTTEAIYQATTGEGITGQYYTKVMGNTMMKEDVNIPKQDCSNKLGSVGGACFRKKTMP
                     IYIVITDEYFTTCPLATYAPNPNNGCMWGWANPEPHSVEETIAAMNGIGAKFIGIDSG
                     FDDSSKKTYQAEEDFELYAQLTGSLDSTGKPFIYHTENSNGTGIGGQIADAVIALTTW
                     IDMDVTTGKMSDDNCYGASAADFIKSSKTITADPVNGVDHWDDKTFYSVTQGTDVTFD
                     VRFYNDFCINNNDNYMEFKAQVTVLGNGSYLSSRLVTVIVPESAPK"
     gene            1682..3397
                     /locus_tag="PL056_05470"
     CDS             1682..3397
                     /locus_tag="PL056_05470"
                     /inference="COORDINATES: protein motif:HMM:NF039815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46498.1"
                     /translation="MKKIIITFLVLLVVSLSGCSVKDDPGETNDNGNSGNSGDSGDSG
                     NTGNTGDTSDTALECGCGDPDDDPDGDGIPNKVEGCNDSDGDGLPDCLDLDSDGDGYS
                     DAEECPSQPCKNSDKDDVPDYLDRDSDNDGLSDKKEKEMGTNPYDKDSDGDGSDDLAE
                     IAYGSDPLKDTDTIPPGIFYVVLPYNAPEDVTRTLTFSTKIEAIDIAIMFDTSGSMSD
                     EFNNLKKEIKTKIVDKINAAFTSPGFTAYGFNTVPWNLRTVVTDDIELVKSKVDETEC
                     DGSGNEIQVQTIHQAATGEGFSSILRTCLNGTCGKIFNVNVPDEIINWPEADCTGQLG
                     SVGGICSRKESMPIYIMITDESLQTCPPEQFLQKWDDCAWAIGQPEGVPIEEAIAAMN
                     GIGAKFIGINSEFDCNDQGTSCSEGDAPDEGFQLIAELTGSLDSQGKSFNTHTKNADG
                     SGMSDQIAEAIISLTTWIDMDVTTGKMSDENCNGLSAADFVKSSKTIKADPADGVSGQ
                     DETTFYSVTQGTDVTFDVRFYNDFCINNFDNFLKFQAQVTVLGNNSYLSSRLVTVIVP
                     EGMSK"
     gene            3417..5228
                     /locus_tag="PL056_05475"
     CDS             3417..5228
                     /locus_tag="PL056_05475"
                     /inference="COORDINATES: protein motif:HMM:NF039815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HPM46499.1"
                     /translation="MKRSFAFLLILIFVSIVSTCGPKDDDNNTGDTGNSGNSGNSGNT
                     GDSGNSGDTGNTGNTGNTGDSGNTGNSGNSGEETCGPGLMKTEWETETWKTADEDNDG
                     IPNSAECSECPCVDTDGDGMPDYIDTDSDGDGYSDSEECPSQPCKNSDKDDVPDYLDR
                     DSDNDGLSDKKEKEKGTNPYDKDSDGDGSDDLAEIAYGSDPLNDGDSIPAGIFYVVLP
                     YNAPEDVTRTLTFSTKVEAIDVLIVFDDSGSMGEEIEILKQEAKTKIIDAIADHFTSP
                     GFAAYGLVMLGWENPYAVYQPITNDAEAVKSAIDKLKGNQGNELHTQAMYLAATGEEY
                     FATLKTCLPAAFGGCDQTYIKPAVYSVPKADCTDQLGSVGGACFRKKTMPIYIVISDE
                     RYDDCISYTPTPPNGIDCIHTQGAPQITVEMTMAAMNGIGAKFIGIDSGFDDQGKETH
                     DPEEFYTLFAQMTGSLDGDGKNFNSHTTSPNGSGMSDQIAQSIIDLTTFIDMDVTTGK
                     MSDENCNGQSAADFIKSSKTITADPVNGVDHWDETTFYSVTQGTDVTFDVRFFNDFCI
                     NNSDNFMKLNAQVTVLGNGSYLSSRLVTVIVPEGDSK"
     gene            5251..6996
                     /locus_tag="PL056_05480"
     CDS             5251..6996
                     /locus_tag="PL056_05480"
                     /inference="COORDINATES: protein motif:HMM:NF039815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46500.1"
                     /translation="MKKSLVIFLALMIILSFQSCGPDDDNDDLGNSGDSGNSGDSGNS
                     GNSGDSGNTGDTGNSGNTGDTGNTGNSGECPCGAADEDDDGDGITNGVEGCIDSDGDG
                     LADCIDLDSDGDGFTDSEECPEQPCRDTDGDEIPDILDRDSDNDGLLDKKEKELGTDP
                     LKKDTDDDGNDDLAEVVYGSDPKDPEDSIPAGIFYVVLPYNGKTDVERELSFSTKIEA
                     IDVMFVIDGSGSMFAEIDQVRDNIKTQIIDTIKTEFPGNEFAAFGLSRITFANTGEFM
                     RQKMTLNTDQLKTALDNFGERQGGMAQGIAELHSPVLYWNANPEGFFGTASLTGFSIK
                     APINLAPADCTNDLGSIGHACFRKKSMPIYIYITDAEHADCGIAPNCSWDSAQELGPT
                     FDDALKMMGAIGAKFIGINTWHEFQDEEETKPADGANPIDDMTMMAQVTGSLDKDGNP
                     FIYQTVDNEGNGMPTQIGEAIVDLTTFIDMNVRTGKMSDEECNGTNAAEFIKSSKTVK
                     AVPPEGVDSFTDVEFISVKQGTEVFFNVKFYNDFCENTTEEPLEFEALVTVLGNGSFL
                     SSRIVTVIVPVNANK"
     gene            complement(7001..8512)
                     /gene="crtI"
                     /locus_tag="PL056_05485"
     CDS             complement(7001..8512)
                     /gene="crtI"
                     /locus_tag="PL056_05485"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019866844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HPM46501.1"
                     /translation="MKKHVIVIGAGPGGLTSAMILAKRGFDVTVFEKDDQVGGRNKEI
                     RLGEYSFDTGPTFLMLKNVLDEVFMEAGADGEKLLNYQKIEPMYRLVYDEKDSINPSS
                     DHEFMKSEIKRLFPGQEKGMEEYLKREKTRFEKMFACLQKPYGSITDYFSKQFINAIP
                     HFSLGKSLMDVLTGYFNEEKAALAFTFQAKYLGMSPWECPGAFALISYIEHAYGIYHV
                     EGGLCKISHAMADVAVKNGAKLHLSTPVKKVITENGKAVGVELENGEKTMADEVIINA
                     DFGYAMTHLFDEGVLKKYTPQKVRSMKYSCSIFMLYLGIDKVYDMPHHAIYFADDYRT
                     NVDDIFKNLKLSDDMSFYVRNTEGVDKTNAPEGHSALYILVPVANLEADVDWEKEKER
                     YTELVIDTVIKKTVMKDLKDHIKAQKVITPLDWQNDYNVYNGATFNLAHNLGQMLYSR
                     PHNQFEDVENVYLTGGGTHPGSGLPTIYESGRISANMICKKYGVKYESMNTLV"
     gene            complement(8540..9904)
                     /locus_tag="PL056_05490"
     CDS             complement(8540..9904)
                     /locus_tag="PL056_05490"
                     /inference="COORDINATES: protein motif:HMM:NF037833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46502.1"
                     /translation="MKSFVIVSVVLLALLNSAGCENKKKDTIYDDGNTGNSGDSGDSG
                     DSGDTGLPDNNTSDDDDIVKKDDDTVMSEDSDLFQNGDNENDEETVDVDSSDDIAVPD
                     EDAEKNDDGGRVMTAVILLPGYSSIHEAGKPFQIKGEVSDTKYPPDQLNVKWEIKKYS
                     GTWTTLVEDKPDSSGITEFQHTIPENDAAYFALRLTLKAPDDTEFMSQEKVFGICTYP
                     FGKIQDFNDPVLGEGWMLSGDAYRDSDGSNGWLELTGDDYNKRGVIYNNLMKVYPGDI
                     TIKLDIRTGQPSVPGDSGADGFAMTVFDSENAVALADYVAAVKTGGCLGYGVAGEYCG
                     LGGETIVDAFHIEFDTWYNSEQGLDDPTTENHIAVNLNGDPGGHQLWKNIFLEDSLWH
                     SIEVTTSGSKVTVKMDGNTLIDNDIEGFSFRGGYIVFSGSTGAAKNLHSVDDLEIVQE
                     CRVP"
     gene            complement(10002..10901)
                     /locus_tag="PL056_05495"
     CDS             complement(10002..10901)
                     /locus_tag="PL056_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HPM46503.1"
                     /translation="MAIIITKENRVIVQGITGREGQVRTKLMREYGSNVVGGVTPGRG
                     GETVMDLPVFDTVEELVENIGAVDTSVIFVPAHLVKDAAIEAILAGVKLLVIVPDRVP
                     VHDVIEIAAYAKKYGADFIGPNTLGVLSVDEGILGMIGGKASSAKAWFKKGNVGVTSR
                     SGGITSSISYYLSQSGVGLSTIVHVGGDGVIGMPHNRVMELFENDPQTELIVMFGEIG
                     TSQEEAVAEMMKAGKLTKPVVAYIGGKGAKKGTRFSHAGAIVEGNKGSWEGKVAALRE
                     AGAHIVENFQEIPAKVLEVLGKK"
     gene            complement(10904..12457)
                     /locus_tag="PL056_05500"
     CDS             complement(10904..12457)
                     /locus_tag="PL056_05500"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HPM46504.1"
                     /translation="MAKLYEYQGKQILKSVKIPIPEGGVAGSVDEAVKQANSIGYPVV
                     VKIQTWSTGRNAVGGVKFADNDLQLKEHVTRMLGMKIGNFAVEKVLVEKKIAIKREFF
                     AGFILNDTDKCPMLLFSSRGGSGIEEIAKEYPESVATMKIDFFNGFKPFEAISLVKKT
                     GVDSAGAAEIGPVLVKMYEGMKKSDARSFEVNPLVQLEDGKIMAADCHVVIDDYAVYR
                     HPEFGIEIAREFDRPPTELERIAYMVEKNDYRGTFFFFQMNEANEFENVIGFHGAGGG
                     GSMMSMNAVSENNCKIANFCDTSGNPPASKVYRAAKVILSQPGIKGYFASGSGVASQE
                     QFHSARGFVKAFLEEDIDIPCSIRLGGNSEDLAIEILEQYLGNKSVNIKGFGKNDSPD
                     KVMKHLLEGMEKNGGAGHKLTPFVLPDVSKCDYSFQTMTGSIHVETAKCETCTSKACI
                     ESCIPKILKDENGSPRLAIDIKEAKKGKCIECLACELACMSKGNQALKIVLPIEGLKE
                     YREKISEGK"
BASE COUNT         3676 a         2768 c         2650 g         3541 t
ORIGIN      
        1 attcaggtga ttcaggtaat tcgggaaatt caggtgattc agggaacaca ggcgatacag
       61 gcaattcagg taataccgga aattcaggga attcaggtaa cagcggtgac acaggaaata
      121 caggcgattc aggagattcg actgttgaat gtccatgcgg agctgatgat gctgacgatg
      181 acggagacgg catcccgaat ggcgttgaag ggtgtaacga tgctgacggg gatggacttc
      241 ctgagtgcct tgacattgat tctgacggag atggaatttc cgactctgag gaatgtccat
      301 ctcagccatg caaaaactca gacaaggacg atgttcccga ctttatggac agggacagtg
      361 ataacgacgg acttagcgat aagaaagaaa aagaaggggg cactaatcct tatgacaagg
      421 attctgacgg tgacggaagt gacgaccttg cggaaattgc atacggttca gacccgttaa
      481 acgatgccga ttcaattccg gcaggcattt tctatgttgt gcttccatac aatgctcctg
      541 aagatgtcac aagaaccctc actttctcaa ccaagataga ggcaatcgat gtgcttatag
      601 tttttgacag aagtgcatca atggatcctg aagctgataa tcttaagaaa gagatcaaga
      661 ccgggatcat tgatgctgtt gctgctcaat tcacttcgcc ggggtttgca gcatacggtc
      721 tgaccagctt cggatatgaa acgatgtaca agatgcatca gacaatgaca ctttcaactg
      781 atgaaataaa atcggctgtt gacggacttg atgcagatga aggaaatgaa atgacaactg
      841 aagcaatata tcaggcaacc acgggcgaag ggatcacggg acagtattat acaaaagtaa
      901 tgggaaacac tatgatgaaa gaggatgtta atattcccaa gcaggattgc agcaacaagc
      961 tcggaagtgt cggcggagca tgtttcagga aaaaaacaat gccgatatac attgttataa
     1021 ccgatgaata ttttacaacc tgtcctttag ctacatacgc accgaacccg aacaatggat
     1081 gcatgtgggg atgggctaat cctgaaccgc actcagttga agagacaatt gctgcaatga
     1141 acggcatagg ggcaaaattc ataggaattg actccggttt tgacgacagc agcaagaaaa
     1201 cctatcaggc agaagaagac tttgagttat acgcacagct taccgggtct cttgattcaa
     1261 ctggcaaacc tttcatttat cacactgaaa actcaaacgg tacagggatc ggcggtcaga
     1321 tagctgatgc agtcattgca cttacaacct ggatagatat ggatgttaca acaggcaaaa
     1381 tgagtgatga taattgttac ggtgcaagtg ctgccgattt tatcaaatca tctaaaacca
     1441 tcactgctga tcctgtaaac ggtgttgatc attgggatga caaaactttc tactcagtaa
     1501 cgcagggaac agatgtaact tttgatgtga gattctataa cgacttctgc atcaataaca
     1561 atgataacta catggaattc aaagctcaag tgactgttct tggcaacgga agttatcttt
     1621 caagcagact tgtgacggta atcgttccgg aaagtgcacc aaaatagtgt tggagtaaaa
     1681 aatgaaaaaa atcattatca cctttcttgt tcttctagtt gtatctttat caggatgttc
     1741 tgttaaagat gatccgggag aaacaaatga caacggaaat tccggcaatt caggggattc
     1801 cggagactca ggcaataccg gcaataccgg tgatacaagt gacacagctc ttgaatgtgg
     1861 ttgcggagat cctgacgatg atcctgacgg agacgggatt ccaaacaagg tagaaggatg
     1921 taacgattcc gatggcgacg ggcttccgga ttgccttgac ctcgattctg acggtgacgg
     1981 atacagcgat gcagaggaat gtccatctca gccatgcaaa aattctgata aagacgatgt
     2041 tcctgactat cttgacaggg atagtgataa tgacggattg agtgataaaa aagaaaaaga
     2101 aatggggacc aacccctacg acaaggactc cgatggtgac ggaagcgatg acctcgccga
     2161 gatcgcatac ggatcagatc cgctaaaaga cacagacacc attcctccgg gaatttttta
     2221 tgtcgttctt ccttacaatg caccggaaga tgttacaaga acccttactt tttcaacaaa
     2281 gatagaagct attgatattg caataatgtt tgacacatcg ggttcgatga gcgacgaatt
     2341 caacaatttg aaaaaagaga taaaaacaaa gatcgttgat aagatcaatg ccgctttcac
     2401 aagtcccggt ttcacagctt acggcttcaa caccgtacca tggaatctgc gaactgttgt
     2461 aaccgatgat attgaattag taaaatcaaa agttgatgaa acagagtgtg acgggagcgg
     2521 aaatgaaatt caggtgcaga ccattcatca ggcggctacc ggtgaaggat tcagcagtat
     2581 tttaaggact tgtcttaacg gaacttgtgg aaaaatattc aacgtaaatg ttcccgatga
     2641 aataataaac tggcctgaag ctgactgcac aggtcaactc ggaagtgtcg gagggatctg
     2701 ttctagaaaa gaatccatgc ctatttatat tatgatcacc gatgaatctc ttcagacatg
     2761 tcctcccgag cagttccttc agaagtggga tgactgtgca tgggcaatcg gtcaacctga
     2821 aggagtacct atcgaagaag caatagccgc tatgaacggg atcggtgcaa aattcatagg
     2881 gatcaattct gaatttgact gcaatgatca gggaacaagc tgcagtgaag gagatgctcc
     2941 ggatgaaggg tttcaactta ttgcagaact taccggatct cttgattcac agggaaaatc
     3001 atttaacaca cacacaaaaa atgctgacgg aagcggaatg agcgatcaga tagccgaagc
     3061 cattatctca ttaacaacat ggatagacat ggatgttacg acaggaaaga tgagcgatga
     3121 gaactgtaac gggttaagtg ccgctgattt tgtaaaatct tcaaaaacga tcaaagccga
     3181 ccctgctgac ggtgtctcgg gtcaggatga aacaactttc tattctgtaa ctcagggaac
     3241 agatgtaaca ttcgatgtac ggttctacaa tgatttctgt attaataatt ttgacaattt
     3301 tctgaaattt caggcacagg ttacagttct tgggaacaac agttatcttt cgagccgtct
     3361 tgtaacagtc atcgtgcctg aagggatgag taagtaataa ttttaggaga agttccatga
     3421 aaagatcttt tgcttttctt ttaattctaa tttttgtgtc aattgtttcg acatgcggac
     3481 ccaaagatga cgacaacaat acgggtgata ccggcaattc aggcaattca ggcaattcag
     3541 gtaacacagg tgattcaggc aactccggtg atacaggtaa tacgggaaac accggaaata
     3601 caggtgattc aggaaatacc gggaactcag gaaattcagg tgaagaaaca tgcggtccgg
     3661 gactgatgaa gacagaatgg gaaactgaaa catggaaaac agccgatgaa gataacgacg
     3721 gaattccaaa ttccgctgaa tgttcagaat gtccctgcgt agatacagac ggcgatggaa
     3781 tgcctgacta tattgatacc gacagcgacg gtgacggtta tagcgattca gaagaatgtc
     3841 catctcagcc atgcaaaaat tctgacaagg acgatgttcc tgattatctt gacagggatt
     3901 ctgacaacga tggattgagc gataagaaag aaaaagaaaa gggaacaaat ccttacgaca
     3961 aagattctga cggtgacgga agtgatgacc ttgctgaaat tgcatacggg tcagatccgc
     4021 ttaatgacgg agattctatt ccggcaggaa ttttttatgt tgttcttccc tataatgccc
     4081 ctgaagatgt tacaagaact ctcacttttt ccactaaagt tgaagcaatt gatgttttaa
     4141 tagtttttga cgattccggc tcaatgggag aagaaataga aattcttaaa caggaagcaa
     4201 aaaccaaaat aattgatgct atcgcagatc atttcacttc acctggtttt gcagcatacg
     4261 gacttgtaat gctcggatgg gaaaatcctt atgctgttta tcagccgata actaatgatg
     4321 ccgaagccgt aaaaagtgca attgataagc ttaaagggaa ccagggcaat gagctgcata
     4381 cgcaggcgat gtatcttgcc gcaacaggag aagaatattt tgcaacactc aaaacctgtc
     4441 tccctgccgc tttcggcgga tgcgatcaga catatatcaa acctgctgtt tacagtgtcc
     4501 cgaaagcaga ctgcaccgat cagctcggca gtgtcggagg agcatgtttc agaaaaaaga
     4561 ccatgccgat atacattgtc atcagtgatg aaagatacga cgactgtatt tcctacacac
     4621 ctactccacc taacggaatt gactgcattc acactcaggg agcgcctcag ataactgtcg
     4681 aaatgactat ggcggcaatg aacgggatcg gcgcaaaatt tatcggtatt gattccggtt
     4741 ttgatgacca gggaaaagaa acgcatgacc cagaagaatt ttatactctt tttgctcaaa
     4801 tgacgggctc tcttgacgga gacggtaaaa attttaattc ccacaccacc agtccgaacg
     4861 gatcaggaat gagtgatcag atagctcagt caataattga tcttactact ttcattgata
     4921 tggatgttac aacgggaaaa atgagtgatg aaaactgcaa cggtcaaagt gctgccgatt
     4981 ttatcaaatc atctaaaacc atcactgctg atcctgtaaa cggtgttgat cattgggatg
     5041 aaacaacatt ttattctgta actcagggaa cagatgtcac ttttgatgtg cgatttttca
     5101 acgatttctg catcaataac agcgataact ttatgaagtt gaacgctcag gtgacagttc
     5161 ttggaaacgg aagctacctt tcaagcagac ttgtaacagt tattgttcct gaaggtgatt
     5221 cgaaataata attaggatat ggagaataca atgaaaaaat ctctcgtaat ttttctggct
     5281 cttatgatca ttttaagttt ccagtcatgc ggaccggatg atgataatga tgatcttgga
     5341 aattcaggtg attcaggtaa ttcaggtgat tcaggtaatt cgggaaattc aggtgattca
     5401 gggaacacag gcgacaccgg caattccggt aatacaggcg acacagggaa caccggcaac
     5461 agtggtgaat gtccctgcgg tgcggctgat gaagatgatg acggtgacgg gataacaaat
     5521 ggcgtcgaag gttgtataga cagtgatgga gacggccttg cagactgtat tgatctcgac
     5581 tccgacggag acggttttac cgattctgaa gaatgccctg agcagccgtg cagggataca
     5641 gacggagatg aaattcctga cattctggat agagacagtg ataacgacgg actgcttgac
     5701 aaaaaggaaa aggaactggg aacagatcct ctcaaaaaag ataccgatga cgacggaaat
     5761 gatgaccttg cggaagttgt ttacggttca gaccctaaag atccagaaga cagtattccg
     5821 gcaggaattt tctatgtcgt tcttccatat aacggaaaaa cagatgttga acgggaattg
     5881 tcattctcaa caaaaataga agcgattgat gttatgtttg ttattgacgg ttcaggcagt
     5941 atgtttgctg aaatagatca agttagggat aacataaaaa ctcagatcat tgacaccata
     6001 aaaactgaat tccctggtaa tgaatttgcc gctttcggcc tttcaaggat aacttttgca
     6061 aataccggtg aattcatgcg gcagaaaatg acacttaaca cagatcaact taaaactgct
     6121 cttgacaact ttggcgaaag acagggtgga atggctcagg gaattgcgga actccacagt
     6181 cctgtacttt actggaacgc aaatcctgaa ggttttttcg ggactgcaag tcttacagga
     6241 ttttcaatta aagctcctat aaatcttgct cctgctgatt gtactaatga tctgggatct
     6301 atcggacacg catgtttcag gaaaaaatcg atgccgatat atatttacat aaccgatgca
     6361 gaacatgctg actgcggaat tgcacccaac tgttcatggg attcagctca ggagcttggg
     6421 cccacctttg acgatgctct gaaaatgatg ggggcgatcg gtgcaaaatt catagggata
     6481 aacacatggc atgaatttca ggatgaagaa gaaactaaac cggctgacgg agcgaacccc
     6541 attgatgaca tgactatgat ggctcaggtt accggctctc ttgataagga tggcaatccg
     6601 ttcatttatc agaccgtgga caatgaaggg aacggaatgc cgacacagat cggtgaggcg
     6661 atagttgatc tgaccacttt tattgatatg aatgtcagaa ccggaaaaat gagtgatgag
     6721 gaatgcaacg ggacaaatgc agctgagttc ataaagtcat caaaaaccgt taaagcagtt
     6781 cctcctgaag gtgttgacag ctttaccgat gttgaattta tttccgtaaa acagggaact
     6841 gaagtgtttt tcaatgttaa attttataat gacttctgtg agaacacaac agaagaacca
     6901 cttgaatttg aagcacttgt cacagttctt ggcaacggat cattcctttc aagcaggatc
     6961 gttacagtaa tcgtccctgt gaacgccaac aaataaagat ctacaccaga gtattcattg
     7021 attcgtattt gacaccgtat tttttgcaga tcatgtttgc agatattctc cctgattcat
     7081 agatagtggg aagtccgctt ccggggtgtg ttccgccgcc tgtgagatat acattttcaa
     7141 catcctcaaa ctggttatgc ggtcttgaat aaagcatctg tccaagattg tgcgcaaggt
     7201 tgaaagtcgc tccgttatag acattgtaat cgttctgcca gtcaagagga gttatcactt
     7261 tctgtgcctt tatatgatct ttgagatctt tcataactgt cttttttata actgtgtcaa
     7321 tgacaagctc tgtatatctt tccttctctt tttcccagtc aacatcagct tcaaggtttg
     7381 caactggaac aaggatataa agagcgctgt gcccttccgg agcgttggtt ttatctactc
     7441 cttcagtgtt tctaacataa aacgacatat catcgctgag tttgagattc ttaaagatgt
     7501 catcgacatt ggtcctgtag tcatctgcaa aataaattgc atgatgcggc atgtcataaa
     7561 ccttgtctat tccaagatac agcatgaaga tcgaacagga gtatttcatt gatcttactt
     7621 tctgcggagt atatttcttt aacactcctt catcaaaaag atgagtcatg gcatagccga
     7681 aatcggcatt tatgatgact tcatcagcca tcgtcttctc accgttttca agttcaactc
     7741 cgactgcctt gccattttca gtgataactt ttttaacagg tgttgaaaga tgcagctttg
     7801 caccattctt aactgcaaca tcggccattg catgagaaat tttacaaagc cctccctcga
     7861 catgataaat tccgtaagcg tgttcgatgt atgatatcag agcaaaggcc ccggggcatt
     7921 cccatggtga cattccgaga tactttgcct ggaatgtgaa ggcaagggcg gctttttctt
     7981 cattaaaata accggtaaga acatccatca gagatttacc gagcgaaaaa tggggaattg
     8041 cattgataaa ctgctttgag aagtaatctg ttattgatcc gtacggcttc tgaagacatg
     8101 cgaacatctt ttcaaacctt gtcttttcgc gctttaaata ttcttccatt cctttttcct
     8161 gacctggaaa aagtcttttg atctcacttt tcataaattc atggtcactt gatggattta
     8221 ttgaatcttt ttcatcatat acgagccggt acatcggctc gatcttctga tagttcaaca
     8281 gtttttcacc atcagctccg gcttccataa aaacttcatc aagaacattc ttcagcatga
     8341 gaaatgtggg gccagtgtca aaagagtatt ctccaagccg gatctctttg ttcctgccac
     8401 cgacttgatc atccttctca aaaacggtca catcgaatcc tcttttcgca aggatcatag
     8461 cgcttgtaag accacccggt cctgcaccga taacaattac atgctttttc atttcttact
     8521 ccctgataac attaaatatt tacggcactc tgcattcctg aacaatttca agatcatcaa
     8581 ctgaatgaag gttttttgca gcccccgtcg aaccgctgaa aacaatgtat ccccctctga
     8641 atgaaaaacc ttcaatgtcg ttatctatca aggtgttccc atccattttg actgtaactt
     8701 tgcttcctga tgttgtgact tctatgctgt gccacaagct gtcctcaaga aaaatgttct
     8761 tccagagttg atgtcccccc ggatctccgt ttaagtttac tgcaatatga ttttcagttg
     8821 tcggatcgtc aagaccctgt tcgctgttat accatgtgtc aaactcgata tggaaagcat
     8881 caactatcgt ctctcctccg agtccgcaat attcacctgc cactccgtat ccgagacagc
     8941 cgccggtttt cacagcggcg acataatcgg caagtgcaac agcattttct gaatcaaaga
     9001 cagtcatcgc aaatccgtct gctcctgaat ccccaggaac tgaaggttga ccagtcctga
     9061 tatccagttt tatagttatg tcaccggggt acactttcat cagattgttg tatataactc
     9121 ctcttttgtt gtaatcatct cctgtcagtt caagccaccc gtttgaaccg tctgaatcgc
     9181 ggtatgcatc tccggacagc atccatcctt cgccaagaac aggatcatta aaatcctgaa
     9241 tctttccaaa aggatatgta catattccaa aaactttttc ctgtgacatg aattctgtgt
     9301 catcgggtgc ttttaatgta aggcgtaatg caaaatatgc ggcatcattt tcagggatag
     9361 tgtgctgaaa ttcggttata cctgaactgt ccggcttatc ttccaccaga gttgtccatg
     9421 ttcctgaata tttctttatc tcccatttta cattcaactg atccggcgga tattttgtgt
     9481 cactcacttc accttttatc tggaaaggtt tccctgcttc gtggattgaa gaatatcccg
     9541 gaagaaggat cacggctgtc atcactctcc ctccgtcatc gttcttttca gcatcttcgt
     9601 caggaactgc aatatcatca ctgctgtcaa catcaactgt ctcttcatca ttttcattat
     9661 caccattctg aaaaagatct gaatcttcag acatcacagt atcatcatcc tttttaacaa
     9721 tatcgtcatc atcagaagtg ttattgtccg gaagccctgt atctccgcta tctcctgaat
     9781 caccgctatc cccgctgttt ccagtgtttc cgtcgtcata aattgtatct tttttcttgt
     9841 tctcgcaacc tgcagaatta agcaaagcaa gaagaacgac tgacactatc acaaaacttt
     9901 tcataaacac ccctttactc tttcactaac aacaaagaat tatatcagtg acttggttta
     9961 tgtcaaaaaa gtggaatgac tattcttaaa aaattgtgat cttatttctt tccgagaact
    10021 tcgagaactt ttgcaggtat ttcctgaaaa ttttcaacta tgtgtgctcc ggcctccctg
    10081 agtgcagcca ccttgccttc ccatgaaccc ttatttcctt cgactattgc tccggcatgt
    10141 gagaatcttg tccctttctt tgcccctttt ccgccgatat atgcaacaac cggctttgtg
    10201 agttttcctg ctttcatcat ttcggcaacc gcctcttcct gcgaagttcc gatctctccg
    10261 aacattacga taagctctgt ctgcggatcg ttctcaaaaa gctccatcac tctgttgtgg
    10321 ggcattccga taactccgtc tccaccgaca tgaacgatgg ttgaaagtcc tactcccgat
    10381 tgagaaagat agtaacttat tgaacttgtg ataccaccgc ttctgcttgt gacaccgaca
    10441 ttaccctttt taaaccatgc tttcgctgaa gatgcctttc cgcctatcat tccgagtatc
    10501 ccttcatcga ctgaaagaac tcccagcgtg ttcggaccga taaaatccgc tccgtatttt
    10561 ttcgcataag ccgcaatttc aatgacatcg tgcactggaa ccctgtccgg aactatcaca
    10621 agaagcttaa ctccggcaag gatcgcttct atcgccgcat ctttgaccag atgtgccgga
    10681 acaaaaatga cggaagtatc aactgcgccg atgttttcaa caagttcttc cactgtatca
    10741 aaaacaggaa ggtccattac tgtctcccct cctcttccgg gagtgacacc gcccaccaca
    10801 ttggagccgt attctctcat aagctttgtt ctgacctgac cttcacgacc tgtgatcccc
    10861 tgaactataa cgcggttttc ttttgtaatt attattgcca tgactatttc ccctctgaaa
    10921 ttttttcacg gtattctttc agtccttcga tcggtagaac tattttcagc gcctgatttc
    10981 cttttgacat gcatgcaagt tcacatgcaa gacactcaat acactttcct ttctttgctt
    11041 cttttatgtc aatggcaagc cgtggcgaac cgttctcgtc tttcagtatt ttcggaatac
    11101 agctttcgat acacgctttt gaagtacatg tttcgcattt tgcagtttcc acatgaatgc
    11161 ttccggtcat agtctggaac gaatagtcgc attttgaaac atcaggaaga acaaacgggg
    11221 taagtttatg gcctgctccg ccattttttt ccatcccttc aagaaggtgt ttcataactt
    11281 tatctggaga atcgttcttg ccgaaaccct ttatatttac tgatttattt ccaagatact
    11341 gttcaaggat ctcaattgcc agatcctctg aattaccgcc tagccggatc gagcatggaa
    11401 tatcaatatc ctcttcaagg aatgccttta caaaacctct tgccgaatgg aactgctctt
    11461 gagaagcgac gccgcttcct gaagcgaaat atcctttaat tccgggctga ctgagtatca
    11521 cttttgcagc tctgtaaact tttgaagcag gaggatttcc tgatgtgtca cagaaatttg
    11581 ctatcttgca gttattttcc gaaaccgcat tcatcgacat cattgaaccg ccgccgcctg
    11641 caccgtgaaa accgatcaca ttctcaaatt cgttcgcttc attcatctgg aaaaagaaaa
    11701 atgtcccgcg gtagtcgttc ttttcaacca tatatgcgat ccgttcaagc tcagtaggcg
    11761 gtctgtcaaa ttctctggct atttcgatgc cgaactcagg atgtctgtaa acggcataat
    11821 catcaataac aacatgacag tcagccgcca tgatcttgcc atcttcaagc tggacaagag
    11881 gatttacttc aaatgatctt gcatcactct tcttcatccc ttcatacatc ttgactaata
    11941 ccggaccgat ttcagccgca ccggcagagt caacacccgt ctttttcaca agcgatattg
    12001 cctcaaaggg cttaaatcca ttgaagaaat ctattttcat cgttgcaaca gattccggat
    12061 attcttttgc gatctcctct attccgcttc cgccgcggga actgaaaagg agcatcgggc
    12121 atttatctgt gtcgttaaga ataaatccgg caaaaaactc tctttttatc gcgatcttct
    12181 tttcaacaag aaccttttca accgcaaaat taccgatctt cattccaagc attcttgtca
    12241 catgctcttt aagctgcaga tcattgtcag cgaacttcac tccacccact gcatttcttc
    12301 cagttgacca ggtctgaatc tttacaacaa caggataacc aattgaattt gcctgcttta
    12361 ctgcttcatc aacacttcca gccacaccac cttcagggat cgggatctta acacttttaa
    12421 gtatctgctt gccctgatac tcatacaatt tagccatgat cagtcacctc acacagttaa
    12481 tcaacaaaag cgaattatcc taactcaaac aattttagat tacaaatttt aagaccctgc
    12541 ctctgtggaa tttagctggt ttgggtttcc agctttttta atagaacgga aaatagtcgc
    12601 tgcatttcaa caagtccact ggtttttgag ctttt
//
LOCUS       DAOOYB010000066        12500 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_5260_length_12500_cov_10.688389, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000066 DAOOYB010000000
VERSION     DAOOYB010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12500
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5260_length_12500_cov_10.688389"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1578
                     /locus_tag="PL056_05505"
     CDS             <1..1578
                     /locus_tag="PL056_05505"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPM46505.1"
                     /translation="QKYVDDPAISAINCSFGPMAGMGNVPVSNAEIESHKNFLQNGRA
                     GLGGAIVYASGNDGVDASYYRLLDYEFSFERNSKNVKAKVMSVGASSGWDTRVAYSNF
                     SETLDIIAPSLSMNPILGIATSYLVGYGDLDDDYTNQFSGTSSAAPVVTGFFGVIFSV
                     NPALTLEEATEILYESADKINPETGFWDAQGHSVKFGYGRVNLHKAVRLAAGLDACEA
                     TATETDNNIDDNCDGFVDEGFGKDLSKVGTKCSANADCANGDFAEADVECLKGEYKAY
                     NFTDGYCTIKNSTFACPDGTGQYSDAQTDKNCFLECNIENKCPEGFSCTDTVLGKCFP
                     KCGGDEDCAEGSYCNDDNQCKQNPSEPGGECESDEGCKYGAMCITQIPGGFCVLMCSS
                     DAQCGDEGAKCVKVDFGGQGAYDICLPSCEEDSECRSFGGMMQMKCHEQYNGKEDVCS
                     MPCEEDADCFDPDAECNNSRCIPIGSATNDDDEVTDGDEKTDGETVDDESTMEDDPVP
                     DEETTKKKSDGCSAIIM"
     gene            complement(1609..1684)
                     /locus_tag="PL056_05510"
     tRNA            complement(1609..1684)
                     /locus_tag="PL056_05510"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1649..1651),aa:Phe,seq:gaa)
     gene            1841..4006
                     /locus_tag="PL056_05515"
     CDS             1841..4006
                     /locus_tag="PL056_05515"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPM46506.1"
                     /translation="MLNLLDKKNSLILRTIFSAAAFILFSIWAVNLDLFLPFKVFFPY
                     FHLIFAFTAVTVFLISKKIISKSGDLKNLKSFFYILPAVGFFASLSISFIFFQGMPHI
                     QDSVDYLVMAQNLKSGKWHSEMPEHYEFFKFLYMIPDGEKFYSMFLPGFSFFLVPFVI
                     LGFPVIANPLLTALNVYLTGMIAKKLFDEKIAVISMQFALMSSFLIVMGGTFMAHSFC
                     AVMTLSAIYFYILSLEKDTWKYPLLTGAAIGWLMLIRPQNAVFIALPLALHALLNIRN
                     DFVFKNGLKTVAAFIPFFGFLMFYNWMYTGNPFIFKQDIYFNYSEPATMCHRFGIGSG
                     CPYSNWIEMPKEGLTWSHAVLVSFRRLSPLIMNLFLHPLTIIFIYIAFLFAKKQSDIK
                     SLSSLFIMFLFTFAGYFFFYFDGNVFGPRYYYETSFLLVIIIAYGFMQTEGHVRVIAV
                     SLVISGFIFQSAVIVPELYHSYKNGFWDIDAKLGEAVKEKGIQNAIVFVSPKHLYGSG
                     FALMDHSDIDKNSVIYGRDLGEKQNSRLMGKYPHRSYYLAKFKKPGKNTEPPKIIEIK
                     PKPDYGNIHIELEDKSYPLTGSPDYCNIFPEKSYLDKYFEMEPPYKYLQFGQWFFFCR
                     FVSTQQYYDFGQKFNDSGKYEMIVKAVSGPEMGKFKVLVDDKYTGVIDFNSPVEEFKL
                     FSVEMKVKKGFRSIKLQPSELVSPLNYFFIDYIEFVPRR"
     gene            4009..5502
                     /locus_tag="PL056_05520"
     CDS             4009..5502
                     /locus_tag="PL056_05520"
                     /inference="COORDINATES: protein motif:HMM:NF017722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004062 - aryl sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HPM46507.1"
                     /translation="MKEFVIFVMLILFTAGCENTAHNTPHTEEAADPDIVLEPDEDSV
                     ETPLIFDVKVEQNEKNVLSCRLTFKTKEKTKIVVRYFSKDHKGYEIVEENEKTDHYFF
                     LWGMRAEKKYTIEIYDSENLNEIIGTSEYATGFLPDTVHYMFLSVNEKDKVSDGFVLF
                     TTSATIVDKANPVALMVDTEGEVVWYFEYYMAGFNVLGDMQFIDETSTIIISLTKGPN
                     MAEIMAEEAIEIDLEGNLLWKSPEAANIYDEPGSWNHIYEWLSDDTILMLRANLESVI
                     QFQNVVNVDRDYNVIWTWSPFDHFETPDCDPSSWCDWTHFNSVSMFKDAGVVYLNSRN
                     MSKYFKIDMNSGNVVWTLGKDGDFTIETENTDPWFEVAHDPEIKSFDGDTVIFYDNGS
                     LERGFSRVIEYKLDFENMTAEISFIYDGTNDGRMWFTEYWGDADSLPNGNIFVTAGDY
                     QLYSNSRLFEVTREGEVVWELFMDKQEDWIVALYNAQKFVPPLKRLD"
     gene            complement(5503..7050)
                     /locus_tag="PL056_05525"
     CDS             complement(5503..7050)
                     /locus_tag="PL056_05525"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPM46508.1"
                     /translation="MLDLVIYIGIATFVAAFIYIIASYAKGLKGSPRELYLMFFTKVT
                     EYSAYGGINIAFILYLSKDMGLSDVSAGTYMGTWSIFLTLLVIIVGPVCDAIGIRKTL
                     LIGSFMLVFSRFLMPFMPNIFLSTFFGFIPMAVGIAITGPVLSVAIKRFTTKETAALG
                     FGLFYTLMNVGFAIGAYIFDLVRHKFGEHAYFSIPLTGLSVSSYQLIILIGFFITIPG
                     MLSILFMRDGVEMTEDNGIVITPGKKIGDGGAFLPALISTVKHTALDTFTKMKQVFSE
                     KPFWIFIFMLFITVFVRLTFQHFHYTFPKYGIRIFGDGVKVGSIYGVLNPTMIVILVP
                     LIAALTKKIGSYKMMLIGTFISAFSVFIAVIPHQYFAFLNNTWVGELIFARWLDVPVE
                     GRSPLFFSVILFVIIFTIGEAFWSPRLMQFTAEIAPKGREASYLSLAVLPFFFAKFFV
                     GPLSGWLVKTYTPEGLPSGVENYPDHYMVWVWVGTTAIISPIGLVVFRKLFTHAEKNI
                     ADAKAAC"
     gene            complement(7197..8813)
                     /locus_tag="PL056_05530"
     CDS             complement(7197..8813)
                     /locus_tag="PL056_05530"
                     /inference="COORDINATES: protein motif:HMM:NF018530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HPM46509.1"
                     /translation="MQTLLSNSVFVLFLIIATGKILEQIKIRNFSLGIASIFIIASIF
                     GYYGFVIPKDFEIFALALFVYCVGIEAGPQFFTMFSKKGRSWIIVPPVYVFFLILFTS
                     LLALAAGGTFSAGSYTGLFAGAFISTPAMASALVRSGDNAIGAAFGIIYPISLIGNVY
                     LISYLPVIFRHNVVKLISLHKEQSENSARSRIFKFFKVTNPNITGKHFGSLTQFKLSG
                     VVFSRYIENGKSFLANDNIILNEGGYVAAVGSPENLENLEILIGPSGIPEIDKDDSVT
                     TAKILISKGNVAGRTLGELDIEDHFNVKITRLIRGSVELSPDKGKQLVLGDKIVVIGN
                     SESIQKLTEFLGDDVNEIFKTQFAPVSIGIVLGMIAGNFPIPGLGYSLGFTGGILAVS
                     MFLGNRVKFLSILWQMPQQTNSFLRQLSLYIFFAALGTSTGGELINIFINPGSTLFVS
                     GAILLAFLPVIFTYGFSTFVLRKDPLETVGLIAGTLNSTSAVLNSNEVLKTDIQNTAF
                     AFAYPVGLILAILSTELFQILSLFIVQRAN"
     gene            9041..9202
                     /locus_tag="PL056_05535"
     CDS             9041..9202
                     /locus_tag="PL056_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46510.1"
                     /translation="MTQPGSVSVEADSNILKGQIVYGDSIAQLIENYTDFCGRMDALP
                     EWMDNGAFK"
     gene            9214..9519
                     /locus_tag="PL056_05540"
     CDS             9214..9519
                     /locus_tag="PL056_05540"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HPM46511.1"
                     /translation="MGYPVVRHPMFHYESDPEVFKLEYQFMLGSDFMIAPVLDQGKNE
                     IEIYLPEGEWVHLWSDLEYINETGIYVTVSAPPGNPAVFYRKGSKYGNKLKDSLKDL"
     gene            9696..11009
                     /locus_tag="PL056_05545"
     CDS             9696..11009
                     /locus_tag="PL056_05545"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPM46512.1"
                     /translation="MERKIYSDLKKWAVQKTRKPLLLRGARQVGKTHSVRNLGKEFEN
                     FVELNFEENRELAVFFSSSLTPQKITDQLSQYTGQSIVPGQTLLFFDEIQACPDALRS
                     LRFFYEKMPDLAVVAAGSLLEFALSEIPSFGVGRISSMFMYPVSFFEFLDATENQLLR
                     KAIQNAGFKNPLSEPLHNKAMKLLRSYMVLGGLPEVVDNYARTGNTVVSQKMIDELIR
                     TFRDDFAKYKTRVATEKIDATFNSTAFQAGSKFVYNAVGDGSTTGFSTALELLNKAGL
                     AEKVFHSSSRGIPLKAQIKPNKFKVLPFDTGVYQRLMGLDIPGFLVADNTEIVNKGAI
                     AEIYVGLMLMQTFPNHMKPDSFYWQREEKGSNAEVDYVIEHNGKIFPIEVKASKRGSM
                     QSMQLFLKERGFHAGIRIAGEKFSSYQNILVVPLYAVENIRELIQ"
     gene            complement(11046..>12500)
                     /locus_tag="PL056_05550"
     CDS             complement(11046..>12500)
                     /locus_tag="PL056_05550"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HPM46513.1"
                     /translation="IVESYETVTISDGIIKGLAADGVISYKGIPYALPPAGDLRWKAP
                     AKSEPWEGTLEAKDYSKACPQSVPIAAGYIPPWDEDCLYLNVFRPFDPKLNDLPVMVF
                     IHGGGYVNGAASLATYEGEWLASQGIVLVTINYRLGQFGFLAVPDTEIVGNFGTMDQI
                     AALEWVKENISAFGGNSENVTIFGESAGGLSVGTMIALRPDLFKRAVIQSATISWGDA
                     MKKEDADDQGIRYIEEAGCIDAENIEKCLKDMTADEVLDTLESGIFDAQSESYGPHVD
                     GELFIDVPYKMAIVGDGKKIPLIIGTNSDEGTAFTIAYQDYIKTEEQYENLIKQQFGL
                     IANQVLTEYPASAYESPWHAYTDIFADLYFNCSSILMTRDFSKYNKNIRYYRFSKVPD
                     YGASTGLGCFHGAELGYLFNTWAENYKKTELGYQETVDNISGLWLKFAADGTLDDWPV
                     YDSEEKYLNISDTLVEEAQLEKKNCDFWSTWYKP"
BASE COUNT         3736 a         2322 c         2745 g         3697 t
ORIGIN      
        1 cagaaatatg ttgatgatcc ggcaataagt gcgatcaact gttcattcgg gccgatggcc
       61 ggaatgggaa atgttcctgt gtccaatgct gaaattgaaa gccacaagaa tttcctccag
      121 aacggcagag cagggctcgg cggagcaatt gtttatgcaa gcggaaacga cggcgtggat
      181 gcttcatatt accggcttct tgactatgag ttctcatttg aaagaaacag taagaatgtt
      241 aaagcgaagg ttatgagcgt cggtgcatct tccggctggg atacgagagt tgcatattca
      301 aatttcagcg aaacgcttga tattatagca ccttctcttt ccatgaatcc catcctcgga
      361 atcgcgactt catatctcgt aggatacggt gatcttgatg atgattatac caaccagttc
      421 agcggaactt cttctgcggc tcctgttgta acaggtttct tcggtgtaat attctcggta
      481 aatccggcac ttacacttga agaagcgaca gaaatacttt atgagagtgc agacaagata
      541 aatcctgaaa caggtttctg ggatgcacag ggacacagcg taaagttcgg ctatggaagg
      601 gtaaatcttc ataaagctgt tcgacttgcc gcaggtcttg atgcctgtga agcaacagcg
      661 acagaaactg acaataacat cgatgacaac tgtgacggtt ttgttgatga aggtttcggc
      721 aaagaccttt caaaagtcgg aacaaagtgt tctgcaaatg ctgactgtgc aaacggtgat
      781 tttgcggaag ctgacgtaga gtgtctgaaa ggagaataca aggcatacaa tttcacagat
      841 ggctactgca ctatcaaaaa ttcaacattt gcatgtcctg acggaactgg acaatattct
      901 gatgctcaga cagacaaaaa ctgtttcctt gagtgcaaca ttgaaaataa atgtccggaa
      961 ggattctcat gcacggatac agttcttgga aaatgttttc cgaaatgtgg cggcgatgaa
     1021 gactgcgcag agggctcata ctgtaatgat gacaatcagt gtaaacagaa tccttcagag
     1081 ccgggcggag aatgtgagtc agatgagggc tgcaaatacg gtgcaatgtg cattactcag
     1141 atcccgggtg gattctgcgt tcttatgtgt tcaagtgatg ctcagtgcgg agatgaaggg
     1201 gcaaaatgtg tgaaagtcga tttcggcggt cagggtgcat acgacatctg tcttccaagc
     1261 tgtgaagaag attctgaatg cagaagtttc ggtggaatga tgcagatgaa gtgccatgaa
     1321 cagtataacg gcaaggaaga tgtctgcagt atgccttgtg aagaggacgc tgactgtttc
     1381 gatcctgatg ccgaatgtaa caattcacgg tgtattccga tcggttccgc aaccaatgat
     1441 gatgatgaag ttactgacgg tgatgaaaaa actgatggtg aaactgttga tgacgaaagc
     1501 accatggaag atgatccggt tcccgatgaa gaaactacaa agaagaaaag tgacggctgt
     1561 tcagcaatta taatgtaatc agaaaaaata tcagaaaaaa atcagaagtg gtgcccaggg
     1621 acagaatcga actgcccaca cggggatttt cagtcccctg ctctaccgac tgagctacct
     1681 gggcaccagc gggcaagtaa ttaccagagt gttaaagcaa agtcaaggaa aaatgaaaat
     1741 gatgcttgat ttagaccttc tttcttgcaa ttgcttcaat aattgataag ctgaaagccg
     1801 ttatctatgg aggtttattg ataaagcact gatgcggaat atgctgaatt tattagataa
     1861 aaaaaacagt ttaattttaa ggacaatctt ttctgctgct gcattcattc ttttttcgat
     1921 atgggctgta aatttagatc tgtttttgcc tttcaaggtg ttctttcctt attttcatct
     1981 gatatttgca ttcactgcag tgactgtttt tctgatctct aaaaagataa tatcgaagtc
     2041 gggtgatctt aaaaatctta aatcgttttt ttatattctg ccggcagtgg gtttttttgc
     2101 gtctctttca ataagtttca tcttttttca ggggatgccg catattcagg acagtgttga
     2161 ttatcttgtg atggcacaga acttgaagag cgggaaatgg catagtgaaa tgccggaaca
     2221 ttatgaattc tttaagtttt tatacatgat ccctgatggt gaaaagttct attcgatgtt
     2281 cctgcccggt ttttcattct ttcttgttcc ttttgtgata ctcgggtttc cggtgatcgc
     2341 caatcctctt ttgacggcac ttaatgttta tctcacaggg atgatcgcaa aaaaactttt
     2401 tgatgagaaa attgcagtaa tttcaatgca atttgctctg atgtcgagtt ttcttatcgt
     2461 tatgggcgga actttcatgg cccattcctt ctgtgctgta atgaccttgt cagcaatata
     2521 tttttatatt ctttcgttag aaaaagatac ttggaaatat cctcttctga ccggtgcggc
     2581 gatcggttgg ctcatgctga taagaccgca gaatgccgtt tttattgcac ttcctctggc
     2641 tttacacgca cttcttaata tcaggaacga ttttgttttt aagaacggac ttaagacagt
     2701 tgccgcgttt atcccgtttt tcggattttt aatgttctac aactggatgt ataccggaaa
     2761 tcccttcata ttcaagcagg atatttactt taattacagt gagccggcaa cgatgtgcca
     2821 ccgtttcggt atcggttcgg gatgtcctta ttcaaactgg atagagatgc ctaaagaggg
     2881 gctgacatgg tcccatgcag tccttgtttc attcagacgg ctttctcctc ttattatgaa
     2941 tctttttctt catccgttga caattatatt tatttacatt gcatttctgt ttgcaaaaaa
     3001 acagagtgat atcaagtcat tatcgtcgct tttcataatg tttctattca cttttgccgg
     3061 atatttcttt ttctatttcg atggaaatgt tttcggaccg agatattatt atgaaacttc
     3121 ttttcttctt gtgatcataa ttgcttacgg atttatgcag acagaaggac atgtaagagt
     3181 tattgcggtt tcgctggtca tttctggttt tatttttcaa agtgctgtaa ttgttcctga
     3241 actttatcac agctataaaa acgggttctg ggatattgat gctaaattag gggaggcggt
     3301 caaagagaaa gggattcaaa acgccattgt ttttgtgtct cccaaacatc tttacggatc
     3361 gggttttgcc ttgatggatc actctgatat tgataagaac agtgtcattt acggaaggga
     3421 tctcggtgaa aaacagaaca gccgtctgat gggaaaatat ccgcatagat catattatct
     3481 tgccaagttc aaaaaaccgg ggaagaacac tgaaccgcct aaaataattg agataaagcc
     3541 aaagccggat tatggaaaca ttcacataga acttgaagac aaaagctatc cgctcacagg
     3601 ttctccggat tactgcaaca tttttccgga aaaatcgtat cttgataaat attttgaaat
     3661 ggaaccgccg tataaatatc ttcagttcgg gcagtggttc tttttctgca gatttgtaag
     3721 cactcagcaa tattacgatt ttggtcagaa attcaatgac tccggcaagt atgaaatgat
     3781 cgtgaaagca gtcagcggcc ctgaaatggg gaaatttaaa gttttagttg atgataaata
     3841 tacaggtgtt attgatttca attcacctgt tgaagaattc aagctttttt ctgttgaaat
     3901 gaaagtgaaa aaagggttca gatcaataaa actccaacca tctgaacttg taagtccttt
     3961 aaattatttc tttattgact atattgaatt tgtgccgcgg agatgatgat gaaagaattt
     4021 gttatatttg taatgcttat tttgtttact gccggctgtg aaaatacagc tcacaacaca
     4081 ccgcataccg aagaagcggc ggatcctgat atcgtccttg agccggatga agatagtgtt
     4141 gaaactccct tgatatttga tgtgaaggtt gaacaaaatg agaaaaatgt tctgtcatgt
     4201 cgtcttacat tcaagacaaa agagaagact aaaattgttg tcagatattt ctcaaaagat
     4261 cataaaggtt atgagatcgt tgaagaaaat gaaaagacag atcactattt ctttctctgg
     4321 gggatgaggg ctgaaaaaaa atatactatt gagatatatg attctgaaaa tctgaatgaa
     4381 attattggaa caagtgagta tgctacgggt tttctgcctg atacagtgca ctatatgttt
     4441 ctttctgtaa atgaaaaaga taaggtttct gacggatttg ttctttttac tacttcagca
     4501 actattgttg acaaagcaaa tcctgtagct ttgatggttg atacggaagg ggaggtggtg
     4561 tggtattttg aatattacat ggcggggttt aatgttcttg gtgatatgca gtttattgat
     4621 gagacatcga ccataattat ttcgcttaca aaagggccga atatggcgga aattatggct
     4681 gaagaagcga tagagataga tcttgaagga aatctgctct ggaaatctcc ggaggcggca
     4741 aatatctatg atgagccggg cagctggaat catatttatg agtggctaag tgatgacacg
     4801 atattaatgc tgagagcgaa tctggaatca gtgatccagt ttcagaatgt tgtaaatgtc
     4861 gacagggatt ataatgtgat ctggacatgg agcccttttg accactttga gacgcctgat
     4921 tgtgatcctt cctcatggtg tgactggaca cattttaatt ctgtttcaat gttcaaagat
     4981 gccggggttg tttatcttaa ttcaaggaat atgtcgaagt atttcaagat agacatgaat
     5041 tcgggaaatg ttgtgtggac attgggcaaa gacggtgatt ttacaattga aacggaaaat
     5101 acggatccgt ggtttgaagt cgcccatgat cctgaaataa agagttttga cggcgatacg
     5161 gtcatttttt atgacaatgg atcgcttgag cgcggcttca gcagggtgat agaatacaag
     5221 cttgattttg agaatatgac cgctgaaata tcttttatct atgacgggac aaatgacggc
     5281 agaatgtggt ttaccgaata ctggggcgat gccgattcac ttcccaatgg aaatattttt
     5341 gtcacagcag gtgattatca gctctatagc aattcccgtt tatttgaagt cacccgtgaa
     5401 ggtgaagtgg tctgggaact tttcatggat aagcaggaag attggatagt ggctctatat
     5461 aatgcccaga aatttgttcc gccactgaaa cgattggatt aatcagcacg ccgcttttgc
     5521 gtcagcgata ttcttttcgg catgtgtgaa aagttttctg aaaacaacaa gacctatcgg
     5581 tgaaattata gcggttgttc cgacccagac ccataccatg tagtgatccg gatagttttc
     5641 aacgcctgag ggaagccctt ccggtgtata tgtcttgaca agccagccgg aaagcggtcc
     5701 gacaaaaaat ttcgcaaaaa agaagggaag aactgcaagt gaaaggtatg atgcttctct
     5761 ccctttcgga gcgatttccg ccgtgaactg catgagccgt ggtgaccaga acgcttctcc
     5821 gatagtgaag attatgacaa atagtattac tgaaaagaaa agaggagatc ttccttcaac
     5881 cggcacatca agccatcttg caaaaatgag ctctccgacc catgtgttat tgagaaaagc
     5941 aaaatactga tggggaatga cagctatgaa aaccgaaaaa gcggaaatga aagttcctat
     6001 cagcatcatt ttgtatgacc cgatcttttt ggtcagggct gcgatcaaag ggacaagtat
     6061 aacgatcatt gtcgggttca gaactccgta aatgcttccg actttcacgc cgtcaccgaa
     6121 gatcctgatg ccgtatttgg gaaaagtgta atgaaaatgc tggaaagtaa gacggacaaa
     6181 aactgttatg aaaagcatga aaatgaatat ccagaaaggt ttttccgaga aaacctgctt
     6241 cattttcgta aaagtatcga gcgccgtatg cttaacggtg cttatcagtg cagggaggaa
     6301 agctccgccg tcacctattt tctttccggg agttataact attccgttat cttcagtcat
     6361 ttctactcca tcccgcataa aaaggataga cagcattccc ggaattgtaa tgaaaaagcc
     6421 gatcagaatt ataagctgat aacttgacac tgaaagtccg gtaagcggaa ttgaaaaata
     6481 tgcatgttct ccgaatttgt gccgcacaag gtcgaaaata taggcaccta ttgcaaaacc
     6541 gacattcata agagtgtaga aaagaccgaa tcccagcgca gcagtttctt tagtggtaaa
     6601 tctttttatg gcaactgaaa gaactggtcc tgttatagcg attcctaccg ccatcggaat
     6661 gaagccgaaa aaagtggaaa gaaagatatt aggcatgaag ggcatcagaa atcttgaaaa
     6721 aacgagcatg aatgagccga tcagaagagt ttttctgatt cctatcgcat cgcatacagg
     6781 tcctacgatt atgacaagaa gtgtgagaaa tatcgaccat gttcccatgt atgttccggc
     6841 tgagacatct gaaagtccca tgtcttttga aaggtaaaga atgaaagcga tgtttatccc
     6901 gccgtatgcg gaatattcgg taactttggt gaaaaacatc agataaagtt cccttggcga
     6961 acctttcaat ccttttgcgt agctggcaat aatgtaaatg aacgcggcaa caaaagtcgc
     7021 aatgccgatg tagataacaa gatcaagcat ccgttactcc tttaaattaa tgaactttca
     7081 ttgataaaaa ggataactat tgtttagaaa ccgtcaagga aaaagctaaa tctaatataa
     7141 attatacatc cttaattttc acttggttaa tgcccgttag cgttatttta attttgtcag
     7201 tttgccctct gaacaataaa tagtgaaagg atctgaaaaa gctcagttga aaggatggca
     7261 agtatcagtc cgacaggata tgcaaaagca aatgcggtat tctggatgtc ggttttaaga
     7321 acttcgtttg aattaagcac tgccgatgtg gaattgagtg ttcctgcaat aagtcccaca
     7381 gtttcaagag gatcttttct gagtacgaaa gttgaaaaac cgtatgtgaa aatcaccggc
     7441 agaaatgcaa gaagaatcgc tccagacaca aaaagagtgc ttcccggatt aatgaaaata
     7501 tttatcagtt caccccctgt gctggttccc agagccgcaa agaaaatata aagtgaaagc
     7561 tgtcgcagaa atgaatttgt ctgctgaggc atctgccaga gtattgacag gaatttgacc
     7621 ctgttgccaa gaaacattga gactgcgagt atccctcctg taaatccgag agaatatcca
     7681 agtcctggta tggggaaatt gcctgcgatc attccgagca cgattccgat cgagacagga
     7741 gcgaactgtg ttttgaatat ttcatttaca tcatcgccaa gaaactctgt aagtttctga
     7801 attgattctg aatttccgat aacaacaatt ttgtcaccga gaaccagctg tttcccttta
     7861 tcgggcgaaa gttcaacaga tccccttatc agacgggtaa tctttacatt gaaatggtct
     7921 tcaatatcga gttctccgag ggttcttccg gcaacattgc ctttggatat gagtatcttc
     7981 gctgtggtca cagaatcatc tttatctatt tcaggaattc ctgacggtcc gataagtatc
     8041 tcaagatttt caagattttc aggacttccg actgcggcga cataaccccc ttcattgagg
     8101 atgatattgt cattggcaag aaaacttttt ccgttttcga tatatcttga aaaaacgaca
     8161 ccgctcaatt taaattgtgt gagtgaaccg aaatgttttc ctgtgatatt ggggtttgtg
     8221 actttgaaaa acttaaagat ccttgatctt gctgaattct cagactgttc tttgtgtaaa
     8281 gagataagtt ttacgacatt gtgtctgaaa attacaggga gatagctgat aagatagaca
     8341 tttccgatca acgatatcgg gtagattatt ccgaaagccg cacctattgc attatctccc
     8401 gatctcacaa gggcggaagc cattgcaggg gtcgaaatga atgctcctgc aaaaagaccg
     8461 gtgtaggagc cggcggaaaa agttcctcct gcggcaagag ccaaaaggga tgtgaacaag
     8521 atcagaaaga acacatagac cggtggaaca atgatccagc tccgtccttt ttttgagaac
     8581 attgtgaaaa actgaggtcc cgcctctatt ccgacacaat atacgaaaag agcaagagcg
     8641 aatatttcaa aatccttcgg gatgacaaaa ccataataac cgaatattga tgcaatgatg
     8701 aatattgaag caattccgag agagaaattt cttattttta tctgttcaag gatcttgcct
     8761 gttgcaatta tcagaaaaag gacaaagaca gagtttgaaa gaagtgtctg catggttcct
     8821 ccaataaaca gaaaaaactt agtccgctcg aatgtataac cgcaggataa taaaatcaat
     8881 aaaaatcaaa ataataaaat catggatttt tatattgatt ttacagggta atcggataga
     8941 ctcgagtgtc attttttttg agtcataatg cctgaaatat tgtttgtcat attaattaat
     9001 tcctgaaaac cctgttacag atgtcaattc ggcctttgat atgactcagc ccggctcggt
     9061 ttcagttgaa gctgattcaa acatattgaa agggcagatc gtttatggtg atagtatagc
     9121 gcaactgatt gaaaattata ctgatttctg cggaagaatg gatgctctgc ctgaatggat
     9181 ggataacggg gcttttaaat agaggcgcag aacttgggat atcctgttgt aaggcatccg
     9241 atgttccatt atgagtcaga tccggaagtg ttcaaactcg aataccagtt catgcttgga
     9301 agtgatttta tgatcgctcc ggtccttgat cagggtaaaa atgaaattga aatttatctt
     9361 cctgaaggtg aatgggttca tctgtggagc gatctggaat atattaatga aacaggaatc
     9421 tatgtgacag tttctgcacc tcctggaaat cctgcagttt tttatagaaa agggtcaaag
     9481 tatggaaata aactgaagga ttctctaaaa gatctttaaa gctcatacca ggtatgccaa
     9541 aatcgcagtt tttcttttca agttgtgtta cgccccgact tttttaaaga tagatgaatg
     9601 atacgccttg acttttttga agacagatgt attatacgcc ttgacttttt taaagatgat
     9661 cctttaagca tttgaatttg ttggcggagg tgattttgga aagaaagatt tattcagatc
     9721 tcaaaaaatg ggcagtgcag aaaacaagaa aaccgcttct tttaaggggt gcaaggcagg
     9781 ttggaaaaac acattcggta agaaatcttg gaaaagagtt tgagaatttt gttgaactga
     9841 attttgaaga aaatcgtgaa cttgctgttt ttttcagttc atctctaact cctcagaaaa
     9901 ttactgatca gctgtctcaa tatactggtc aatcaattgt tccggggcag acacttttgt
     9961 tttttgatga aattcaggca tgtcccgatg cattaagatc gttaaggttt ttttatgaaa
    10021 aaatgccgga tcttgctgtt gttgccgcag gatcgctact tgaattcgca ttgtctgaaa
    10081 ttccgtcttt cggagtggga aggatctctt caatgttcat gtatccggtt tcattttttg
    10141 aatttcttga tgcgacggaa aatcagcttt taagaaaggc catacagaat gccggtttta
    10201 agaaccctct ttctgagccg ctgcataata aagcgatgaa actgttaaga tcatatatgg
    10261 ttctcggggg tttgccggaa gtagttgaca attatgccag aactggaaat acggtcgttt
    10321 ctcaaaaaat gatagatgaa ctgatacgca ctttccggga tgatttcgca aaatataaaa
    10381 caagagttgc aacagaaaaa attgatgcaa catttaattc cacagctttt caggcaggat
    10441 caaagtttgt ttacaacgct gtcggtgatg gttccacaac tggtttttca acggcacttg
    10501 agcttttgaa caaagcgggg ctcgcagaaa aagtatttca ttcatcaagt cggggcatcc
    10561 cgttaaaagc acagataaaa cccaataaat tcaaagtcct tccgtttgac acgggtgtct
    10621 atcaacggct gatggggctt gatattccag gttttcttgt ggcggataac acagagattg
    10681 taaacaaggg agctatagct gagatttatg tcggtcttat gcttatgcag acatttccaa
    10741 atcacatgaa acccgatagt ttttactggc agagagaaga aaaaggaagc aatgccgaag
    10801 ttgactatgt aatcgagcac aatggaaaaa tattccctat agaagttaaa gcatcaaaaa
    10861 gaggaagtat gcagagcatg cagctttttt taaaagaacg gggttttcat gctggaataa
    10921 gaattgccgg agaaaaattc agcagttatc agaatatttt agttgttccg ctatatgccg
    10981 ttgaaaatat aagagagctt attcagtagc aaaagcataa acatatgtaa gcatatctta
    11041 tctttttaag gtttgtacca agttgaccag aaatcgcagt ttttcttttc aagttgtgct
    11101 tcttcaacaa gagtatcaga aatgttaaga tatttctctt cactgtcata aaccggccag
    11161 tcatcaagtg ttccgtctgc cgcaaattta agccagagtc cgcttatgtt atcgactgtt
    11221 tcctgatagc ccagttcagt ttttttataa ttttcagccc aggtgttgaa aagatatccg
    11281 agctctgctc cgtgaaaaca cccaagacct gtggaagcac cgtaatcggg aacttttgaa
    11341 aatctgtaat accttatgtt cttgttatat ttggaaaaat cgcgtgtcat gagaatcgat
    11401 gaacagttga agtaaagatc tgcaaaaata tctgtgtatg catgccatgg tgattcgtaa
    11461 gctgatgcag gatattcagt cagcacctga tttgcaataa gcccaaactg ctgttttatg
    11521 agattttcat attgctcttc tgttttgatg taatcctgat aagcgatagt gaaagctgtc
    11581 ccttcatcac tgtttgtacc gattataaga ggaattttct ttccatcccc gacgatcgcc
    11641 attttgtaag ggacatcgat gaagagttcc ccgtcaacat ggggaccgta gctttctgac
    11701 tgagcatcaa aaatgccgct ttcaagagta tcaagaactt catctgccgt catatcttta
    11761 agacatttct caatattttc agcatcaatg catcctgcct cttcaatata acggatcccc
    11821 tgatcatcgg catcctcttt tttcattgcg tcgccccagg aaatggtcgc actttgaatt
    11881 acagctcttt tgaaaagatc ggggcggagc gcgatcattg ttccgacaga gagtccacct
    11941 gccgattctc cgaaaattgt gacattttca ctgtttccgc cgaaagctga aatgttttct
    12001 tttacccact caagtgccgc gatctgatcc attgttccga aattgccgac aatttcagta
    12061 tcaggaacag caagaaatcc gaactgtccg agacgatagt tgatcgttac aagaacgatt
    12121 ccctgagatg caagccactc tccttcataa gttgcgagcg atgccgcacc attgacatat
    12181 ccgccgccat gaatgaaaac cattactgga agatcattta attttggatc aaatggtctg
    12241 aaaacattaa gataaaggca gtcttcatcc catggaggaa tatatcctgc cgcaataggt
    12301 actgactgag gacatgcttt ggaataatct ttcgcttcaa gagttccttc ccacggttca
    12361 cttttcgctg gtgctttcca acggagatct cccgccggag ggagggcgta agggatccct
    12421 ttatagctta taacaccatc tgccgcgagc ccttttatta ttccgtcact gatcgtaact
    12481 gtttcataag attcaacaat
//
LOCUS       DAOOYB010000067        12460 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_5284_length_12460_cov_9.431439, whole genome shotgun sequence.
ACCESSION   DAOOYB010000067 DAOOYB010000000
VERSION     DAOOYB010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12460
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5284_length_12460_cov_9.431439"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..964)
                     /locus_tag="PL056_05555"
     CDS             complement(<1..964)
                     /locus_tag="PL056_05555"
                     /inference="COORDINATES: protein motif:HMM:NF015210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HPM46514.1"
                     /translation="MESFDSTKRGLYKILEDVDSGKIQLPDFQRKWVWDDDRIKGIIA
                     SVAKSFPIGAVMLLETGNESIRFKTKPIEGVVGADNKKPEMLILDGQQRITSLYQAII
                     SNRVVNTRNSKGYEIKRWYYIDMKKALDPDTDLEEAVFSINENKIITENIGRDIVLDL
                     TTQEKEFQNLMYPVCMVDEYDEWRTRFSEYWDYEREKIKFWNEFEKKIIKSFNGYMLP
                     IIEMKKENPKEAVCQVFEKVNTGGISLTVFELLTASFASEEFDLKEDWEKVKKKFEPY
                     KVLLSTSNTDLIQAVTLFSTYKKRQGLIKLKFPQKKFSRFLTEEE"
     gene            complement(1244..2680)
                     /locus_tag="PL056_05560"
     CDS             complement(1244..2680)
                     /locus_tag="PL056_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008617964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM46515.1"
                     /translation="MKEIEHQNIEWKESWRDEYLKWICGFANADGGTLVIGKRDDGTI
                     AGIANAKKLMEDIPNKVKDIMGIMVDVNLKKSKEGEYIEIIVEPYHNPVSYKGEYHYR
                     SGSTKQELKGAALDRFLLRKYGRNWDSVPVPYVKVEDLSAEAFAHFRKLAKKGHRIDE
                     ELLSEPNKYLLEKLHLYEGSYLKRAAVLLFHPDPEKFITGSYVKIGFFRNNTDILYHD
                     EVHGDLFTQSKKTLEILTTKYLRALISYEGIQRVETLQVPNDALREAILNALVHRDYA
                     TGAPIQIRVYSDKLMIFNSCVLPDNWTIKNLVGKHSSHPYNPDIANAFFRAGEIESWG
                     RGIERIFTACKNEKAPKPKLELEGTHGLWIEFPFIQLSEEEMSGKMSGKTSGKTSGNK
                     LGNSKDELREKYGRSTEEIRKKYGIIAEKIILNMIENSHITIKEIAVLLSVSQSAVEK
                     NIAKLKKDALLQHIGSTKGGHWEVLEEK"
     gene            complement(2795..4141)
                     /locus_tag="PL056_05565"
     CDS             complement(2795..4141)
                     /locus_tag="PL056_05565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPM46516.1"
                     /translation="MSENFYKRALAVAIPVAVHNLIISSINMADVFMVGQLGESSIAA
                     LGISNQLMFLFILFMFGMGSGGSIFISQFFGKKDRVNLHRVIGIMMVPAVAISIIIAA
                     GAVFIPEILMSVFSNDAEVLDQSGRYLRIVGLSYPLTAVSFIFQIVLRSTDKPRIPMY
                     TSFFALLINISFNWLLIFGNMGFPKLGIEGAAIATVLARGLETVLTIFIVYYTKSAAA
                     AKLRVMLDFKKDLVKRVFITSMPVFINESVWASGIVALNIIYASLGTQSIASISIMDS
                     VGRISFAAFIGIANAGAVILGTDIGAQKYDQVWEHSKKFSAMSIYAGIVVSFLLFITA
                     PSVISIYNISPTVADLAYKTILVWVFIIPIVSFNCLNIVGILRSGGDTKAALWIDIGA
                     LWAIGVPAALIGASWFQLPIYWVYLLAKSEEIFKFFFGMKRYLSKKWIRNLVEDVE"
     gene            4276..5040
                     /locus_tag="PL056_05570"
     CDS             4276..5040
                     /locus_tag="PL056_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46517.1"
                     /translation="MRSLTKSMTLIVIAVLLSVVTGCWDDSNKATGDLGVLKYYLTTK
                     YDADGNLKTGILPTNISHRIGAYSTGEEYLGKYDEIYHIVEPAENAVLINHDNTYDVP
                     DFTIKVTKAGSYKITTKNDEKVLDMITLKFEDPASLEVITKVRVAYGDSFDVMPSTGT
                     ITVEEGSQVAFIPYLKTTSGTRMIGDVKCKFTADPDWMVVPDVEDTYITEDTSIAFGY
                     ESTFYFIEEGTVTFNFKHTESGYTGSQKFDVTAVSK"
     gene            5158..6096
                     /locus_tag="PL056_05575"
     CDS             5158..6096
                     /locus_tag="PL056_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46518.1"
                     /translation="MSPDQQLERSAYREAGRAVMSYFAGYSCRHLTVTEKNSTCNLND
                     FDFGSDTPMINAVSRYKDDPSIYEAFPENIQTRCKDVSLKIIIIIMGAPAGEAIYKNN
                     GKFAGSPLLSIPAQDLEIADKIDYFLSIVKYGQHPGNYLQMIFGQVLQLMEGKEVWNA
                     AYALASAIMGSTDHKLEKNEIEKILVETGFLSYLCHLRQGSQQVPKTPSSHSGFSREE
                     LEKMKKKNQNASLLNKDDAVTKIVSFAKNYKFGQLQIGLAEDDLKDLSGLEKIIVETQ
                     SHNIAKEVMMYFVCLGMKISPLSSKSATASKVFVNF"
     gene            6240..7442
                     /locus_tag="PL056_05580"
     CDS             6240..7442
                     /locus_tag="PL056_05580"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HPM46519.1"
                     /translation="MKRFLAFISFFIVITLSAGDSIKDEDRASLTDLLTELTKAKPDK
                     ILNDIKVWPGESSIRVAIYPSDKVKYKYNLLKGEDKDRIYIDLLAVDADGFTLPDVKY
                     DSFLKGIRMGKRDTGIRIVLDTGKVESYNVIEMEEPWRIVIDYYGKKPVEVEKKSEVK
                     ESKVSEKVKEKEQKKEPQAKKKKVVVIDPGHGGKDPGAVVGKLYEKDIVFNLAKRIKK
                     LSQKYDSIEIKLTRDKDIFLPLEERAAIANTMNGDLFISLHANSFSDENVGGLEVYHL
                     DNTRDNYTDKLAMVENKISDSSSLLNTILVDMTMSYFVKDSLNYATSIGIGLKENLKK
                     HNVKVRGYKKGALFYVLVGARMPSLLLEIGYLTNKMERGLLLKDNYLDDIAVSVLDAI
                     AKTANEKK"
     gene            7462..9300
                     /locus_tag="PL056_05585"
     CDS             7462..9300
                     /locus_tag="PL056_05585"
                     /inference="COORDINATES: protein motif:HMM:NF017722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004062 - aryl sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HPM46520.1"
                     /translation="MKRFLTGFFQILLVFVFIVSCGENKTNEKDDDNVTDNEVSDNED
                     TDNESNDEWELFDEPVLNPSGNIPLSASISTWLGGGQTSVSVAVKDIDGVSKDFEKEY
                     SLVGLSDRAEIPVLGLFPDHENIVVIKAFDKDKKLVAEREFKIKTAPLPKNFPEVELT
                     GKIESGWTIVNWLFTPRERPEMSAIAVDQSGRIRWYTDFPYAAAFPITVHDGYLYTSD
                     GASILYKYDFMGNEIEKWDVEPHGYTEIHHEIIIKDDGNIILGVSKKDDPWIEDRVIE
                     INPENDQLRGTWDLRKTFPDVCDLFYDVPLTEGDDPAGQKNDPVHNNAIWYNAEDDSI
                     ILGSQRSGVAKITHSGYLKWFLAPHIVAVIDDEDSDGFSDSLIDGYDEADPTTHIGDF
                     KGDKYVIDRMPVAGKPHEVYSSFDFRYQEFLLTPVDKDGDEITDVEVLNGFKDHEDFA
                     WPFRQHTPTLLKNGNLMVFDNGLARNFGYPPIYSTHYSRAVEYEITPDKEDGYGGTVK
                     QVWEYVLQNDPMWYSLSIVVSGANEIENGNRLITSGSIGSSFFPDQLKVMYGDGPVGA
                     LIVEVDPKDNTELNQLFFKRYIDDEYPNTQFSAYRAYRFELSAKLR"
     gene            complement(9305..10795)
                     /locus_tag="PL056_05590"
     CDS             complement(9305..10795)
                     /locus_tag="PL056_05590"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HPM46521.1"
                     /translation="MKNRGQNRIFFILIAVAVLLVLLVVFHDTPQVEESAVEENTVIP
                     EKVEESSPTAETAKINEPVAEPVAEPVAETAPASVEPGKKGDEEGDAAVPDEPIEKDK
                     NSEIDAEGFIHGKILPKKGFEEALLKLPGIQLSHTMEIINAIRYDVDLSQIKAGEDFK
                     VKLSNEGVVEEFVFYPDIITFHILKRDPADRKLKYTQKIMPTEKRYRIIEGEIETTLN
                     QALIDREDVTRTIRGVTNGILECIVSFRTDARKGDRYRILVEDKFYNDQMVPGSKILY
                     ASYEGKRAGLNEAFRFEDTDPKSAFNAHYTKEGKALIPSAMRLPVDRVHITSPFGMRR
                     HPVTGRRAFHNGVDYGGPVGDPIYAVAPGKVIDVKTTDYGGKQITIQHADRSQTYYLH
                     LNSFYVRAGSTVGPRQKIGTMGRTGRVTGPHLHFGIKSPKGEWLNPLKIKMIASPQLD
                     GTRFSKFKAQMKDTVILLNDTEKYQEWLRKYDEGPQPEEFYKQYEL"
     gene            complement(10891..12459)
                     /gene="nadB"
                     /locus_tag="PL056_05595"
     CDS             complement(10891..12459)
                     /gene="nadB"
                     /locus_tag="PL056_05595"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HPM46522.1"
                     /translation="MGSGLSGLSTALSLAEKNAKVLIVTKESIDVSNSANAQGGIASV
                     VDFGHDSFEKHIKDTISAGAGLNKEDVVRFFVENGPDAINYFKDLGCVFTENEQGTLD
                     LGKEGGHTERRVVHAGDMTGMELIRAIKKGVLNHPEITVLENHTAVDLITTSKFMLSD
                     HNRIIGAYVLNDKENVITIIAKAVLLATGGCGKVYLYTSNPDVTTGDGVAIGFRAGVS
                     AVNMEMIQFHPTILYHEKVTSMLISEALRGEGGVLVDKRGHSFMEKHHSLKSLAPRDI
                     VARAIDFEMKKSGDDCVFLDMTKLDGNFLRKRFPNIFRVCLDAGIDMRTQPIPVVPAA
                     HYMCGGLKSDVSGKTSLPGLFISGEIAHTGLHGANRLASNSLLEAAVMAHAAGKEILQ
                     YLGSSTKIVQEPPDWNDESAGSIDEKIIIKQNWDEIRRFMWNYVGIVRSDKRLERALK
                     RLELIKHEINEYYWNFKITRDLLELRNITLVAEIIIKSAMKRKESRGLHFNIDYPETR
                     NEAIDTEILPEEIL"
BASE COUNT         3597 a         2581 c         2508 g         3774 t
ORIGIN      
        1 cctcctcttc agttagaaat ctgctgaatt ttttttgggg gaacttcaat ttaatcagtc
       61 cttgtctttt cttatatgta gagaataatg taactgcttg aataaggtcg gtattacttg
      121 tacttaaaag aaccttatat ggttcaaatt ttttttttac tttctcccag tcttccttaa
      181 ggtcaaattc ttctgatgca aaagatgctg tcaaaagttc aaaaacagtg agagatattc
      241 cacccgtgtt cactttttca aaaacctgac agactgcttc ctttggattt tcttttttca
      301 tttcaattat gggaagcatg tatccattaa agcttttgat tatttttttc tcaaattcat
      361 tccaaaattt tattttctct ctttcataat cccaatactc tgaaaatctt gtgcgccatt
      421 catcgtattc atcaaccata caaactggat acatcaaatt ttgaaactct ttctcctgtg
      481 tagtgagatc tagaacaata tctcttccaa tattctctgt aataattttg ttttcgttga
      541 tagaaaaaac tgcttcttcg agatctgtgt ccggatcgag tgcttttttc atatcaatat
      601 agtaccatct ttttatttcg taaccctttg aatttctagt gtttacaact ctattcgata
      661 ttattgcctg atataaagaa gttattcttt gttgtccatc caaaattaac atctcaggtt
      721 ttttattatc agccccgacc acaccttcga taggttttgt tttaaacctt atactttcat
      781 ttccagtttc aagaagcata actgcaccaa tgggaaagga ttttgcaact gaagctataa
      841 ttccttttat gcgatcgtca tcccacaccc acttacgctg aaaatccggc aattgaatct
      901 ttccagaatc cacatcttct aaaattttat aaagccctct ttttgttgaa tcaaaagatt
      961 ccatctgctg tctccttttt tttaaattaa aatttttttc cattttgagg cgtaatcaat
     1021 gtcgccccta caaaaaaaat caatgttttt catcccattc ctcacatgta agggcgaaaa
     1081 atttttcgcc ctgtttcagg gttgaaacga ggttcaaccc gtacagcaca tggttcaacc
     1141 cgtacagcac atggttcaac ccgtacgaaa aaaccaaaca cctattgggc gtcatcaatg
     1201 tcgcccctac gaaaaccaat aaatccaaat tttcttaact tcatcatttt tcttccaata
     1261 cttcccagtg tccacctttt gtggaaccaa tatgttgcaa caaagcatct ttcttaagtt
     1321 tggcaatgtt tttctctaca gcactttgac tgactgaaag aagcactgca atttctttga
     1381 ttgtaatatg tgagttttct atcatattaa ggattatttt ttctgctatt ataccgtatt
     1441 tcttccgtat ttcttccgta cttcttccgt acttctccct caattcgtcc ttactattac
     1501 ccaacttgtt tcccgacgtt ttccccgacg tttttcccga catttttccc gacatttctt
     1561 cttctgagag ctgtatgaaa ggaaattcta tccacagacc gtgcgttcct tcaagttcca
     1621 gctttggttt tggtgctttt tcattcttgc atgcggtaaa aatccgttca ataccgcgtc
     1681 cccacgattc aatttcacca gcacgaaaaa acgcattggc aatatctgga ttgtagggat
     1741 gagaagagtg tttgccgaca agatttttta ttgtccagtt gtcaggcagc acacacgaat
     1801 tgaaaatcat cagtttatct gaataaaccc tgatctgaat gggtgctccg gttgcataat
     1861 cacgatgaac gagagcatta agaatagctt ctctgagtgc atcattaggc acttgaagtg
     1921 tttcaacacg ctgaattcct tcataactta tgagtgcacg cagatatttt gttgtaagta
     1981 tttcaagtgt ttttttagac tgagtgaaga gatctccatg aacttcatca tggtaaagaa
     2041 tatctgtatt gttcctgaag aatcctattt ttacataaga gccggttata aatttttcag
     2101 gatcaggatg aaaaagaagt actgccgctc tttttagata gcttccttcg taaagatgta
     2161 gtttttcgag aagatattta ttcggttcgc tgagcagttc ttcatcaatt ctatgacctt
     2221 tttttgcgag ttttctgaaa tgagcaaatg cctcagctga aagatcttcc acctttacat
     2281 aaggcaccgg cacactgtcc cagtttctgc catatttgcg aagcaaaaag cggtcaagtg
     2341 cggcaccttt taattcctgt tttgtactgc cactcctgta atgatattcg cctttgtaac
     2401 tcaccgggtt gtgatagggt tcaacaataa tctcaatata ttcaccctct tttgattttt
     2461 tgagattaac atcaaccata atgcccatta tgtctttgac tttgttggga atatcttcca
     2521 taagtttttt tgcatttgca attcctgcaa tagtcccatc atcccttttt ccgataacaa
     2581 gtgtgccgcc gtcagcattt gcaaaaccgc aaatccattt aagatattca tctcgccacg
     2641 attctttcca ttctatgttc tgatgttcga tttctttcat tcactccccc ctcttcattt
     2701 cctcacttct gaatcagatt ctgaaagatt ttaacgattg ttattttgtt ggcaattaat
     2761 ttgttttttg gtaagggcga aaaatctttc gcccttactc aacatcctcg acgagattgc
     2821 ggatccattt ttttgagaga taccgtttca ttccaaagaa aaatttaaag atctcttcgc
     2881 tttttgcaag aagatagacc cagtaaattg gaagttggaa ccaacttgct ccgatgagtg
     2941 ctgctggaac tccgatcgcc cagagtgctc cgatatcgat ccagagtgct gctttggtgt
     3001 cgccgccgct tcgcagaatt cccacaatat tcaggcagtt gaatgatact atcggaatga
     3061 tgaacaccca gacgaggatg gttttatatg caaggtcggc aactgtcggt gaaatgttgt
     3121 atattgaaat gacggaaggt gctgtgatga aaagaagaaa actcacaacg attcctgcat
     3181 agatcgacat ggcggagaat ttctttgaat gttcccacac ctgatcatat ttctgtgctc
     3241 cgatgtcagt cccaagaatt actgctccgg cattggcaat tccgatgaat gcggcaaacg
     3301 agatccttcc gacgctgtcc attatgctta ttgatgcgat agattgtgtt ccgagcgatg
     3361 cataaatgat gttaagtgct acaattccgc ttgcccagac cgattcattg atgaaaacag
     3421 gcatggaagt tataaacacc cttttcacaa gatctttttt aaaatcaagc atcactctga
     3481 gttttgcggc tgcagcagat tttgtgtaat aaacgatgaa gatcgtaaga actgtttcaa
     3541 gccctctggc aagcactgtt gcaattgccg ccccttcaat tccaagcttt ggaaaaccca
     3601 tattcccgaa tatcagaagc cagttgaacg aaatgtttat caaaagtgcg aaaaaagatg
     3661 tgtacatcgg aatgcggggt ttatcagtgc ttcttaaaac tatctgaaaa atgaatgaga
     3721 ccgcagtcag cggatagctt aatcccacaa tgcgaagata ccttcctgac tgatcaagaa
     3781 cttctgcatc attagaaaag accgacatca gaatttcagg aatgaaaacc gcccccgcgg
     3841 caataataat tgagatcgca actgcaggaa ccatcatgat cccgataaca cggtgaagat
     3901 ttacccggtc ctttttgccg aagaactgcg aaatgaaaat tgaaccgccg cttcccattc
     3961 cgaacatgaa caggatgaaa aggaacatga gctgattgct tatccccaga gccgcaattg
     4021 acgattcacc gagctgcccc accatgaaaa catcggccat gtttattgat gaaattatca
     4081 gattatgcac tgcaaccggt atcgctaccg ccaaagcccg tttgtaaaag ttctcagaca
     4141 tcgcattccc tcaaaaaaac ggcgcattct aatgcaaagg gctgaagtgt caaaatataa
     4201 atgatgaata taataaatcc tcccgcgaca tgtcactaaa caatcgtttt tgatatttaa
     4261 tttatggagg ggaaaatgag atcgttaaca aaatcaatga ctttgattgt tattgcagtt
     4321 ctattgtctg ttgtaacagg ttgctgggat gattcaaaca aggcgacagg ggatcttgga
     4381 gtgcttaagt attatctcac gacaaaatac gatgctgacg gcaatttgaa gacagggata
     4441 cttccgacga acatttctca ccggatcggt gcatattcga caggcgagga gtatctgggc
     4501 aaatacgatg agatatatca cattgttgag ccggcggaaa atgctgtgct gatcaaccat
     4561 gacaacactt acgatgtgcc tgatttcact atcaaagtta caaaagccgg ctcttataag
     4621 ataacaacga aaaatgatga aaaagtgctt gatatgataa ccctcaaatt tgaagatccg
     4681 gcatcacttg aagtgatcac aaaagtgaga gtggcttacg gagattcctt cgatgttatg
     4741 ccgtcaaccg gaacgataac tgttgaagag ggttctcaag ttgctttcat cccgtatctt
     4801 aaaaccacat caggaacgag aatgatcgga gatgttaaat gtaaattcac ggcagatcct
     4861 gactggatgg tcgttcccga tgttgaagac acatatatca ctgaagacac ttcgatcgct
     4921 ttcggatatg aaagcacttt ctatttcatt gaagaaggga cagtcacttt caatttcaaa
     4981 catactgaat cgggatatac cggatcacag aagttcgatg tcacggcagt ctccaaataa
     5041 ttaaaaactt taattttaca caatcagaat aattttttca ttaagtaaaa caatttctgg
     5101 cacctttttt gcataaagta aaacaggtta ttgctctaaa ctttgacagg agttaaaatg
     5161 agtcccgatc agcaacttga aagatcagct taccgcgagg cagggagagc ggtaatgtca
     5221 tattttgccg gttattcatg caggcatctt actgtaactg agaaaaattc cacctgtaat
     5281 ctcaacgatt ttgatttcgg aagcgacact cccatgataa atgcagtgtc acgatacaag
     5341 gatgaccctt caatttacga agcttttccc gaaaacatac aaacaagatg caaagatgta
     5401 tctctaaaga tcataattat cataatgggt gctccggcag gagaagccat atataaaaat
     5461 aacggaaaat tcgcagggag ccctcttttg tcaattcctg cacaagatct tgaaattgct
     5521 gataaaattg attattttct ttcaatcgtc aaatacggtc agcatcccgg caactacctt
     5581 cagatgatat tcggacaggt tcttcaactt atggaaggaa aagaggtgtg gaacgcggct
     5641 tacgccctgg catctgccat aatgggttca actgatcaca aactggaaaa gaacgagata
     5701 gagaaaatac ttgtcgaaac tggattctta agttatctgt gccacttaag gcaaggttcg
     5761 caacaagttc caaaaacccc ttccagccat tccggctttt caagggaaga gttggaaaaa
     5821 atgaaaaaga agaatcagaa cgcatcactt ctgaacaaag atgacgcagt gacaaaaatt
     5881 gtcagctttg ccaaaaatta taagttcgga caacttcaga tcggtctggc cgaagatgat
     5941 ctcaaagatc tttcaggttt agaaaaaata attgttgaaa cacagtcaca taatattgca
     6001 aaagaagtta tgatgtattt tgtctgtctt ggaatgaaaa tttctccgct ctcatcaaaa
     6061 agtgcaacag cttcaaaagt ttttgtcaat ttctgatcat tgaaaacctg agaatttatc
     6121 cttaattttt gaaaaaagtc aatcagtgaa gattgacacc ctattttaaa tctgataatg
     6181 tctcgtgctt tgaacaattg cttatttcgt tgtggttaat tcaggcaggt gtttttggga
     6241 tgaaaaggtt tttagcattt atctcttttt ttattgttat aacgctttct gcgggtgatt
     6301 caataaaaga tgaggacagg gcatcgctta ctgatcttct gactgagcta acgaaagcaa
     6361 aacctgacaa aattctcaat gatatcaagg tttggccggg tgaaagttcg attcgtgtcg
     6421 cgatatatcc aagtgacaaa gtgaagtata aatacaatct gctcaaaggt gaggacaagg
     6481 atcggatcta tatcgatctt cttgcagttg atgctgacgg tttcacactt cccgatgtta
     6541 aatacgactc atttctcaaa gggataagga tgggaaagag agatacgggg ataaggatag
     6601 ttctcgatac aggcaaagtc gaaagttaca atgtcatcga gatggaagaa ccgtggagaa
     6661 tagtgataga ctactacgga aaaaaacctg ttgaagttga gaagaagtcg gaagtaaaag
     6721 aaagtaaagt gtctgaaaaa gttaaggaaa aagaacagaa aaaagagccg caggcaaaaa
     6781 agaaaaaagt tgtggtcatt gatcccggtc atggcggaaa ggacccaggt gcagttgtcg
     6841 gaaagcttta tgaaaaggac atagttttca atcttgcaaa aaggataaaa aaactttcgc
     6901 aaaagtatga ctcgatagaa attaagctta caagagataa agacattttt cttcctcttg
     6961 aagaaagggc ggcaatcgca aacacgatga acggagatct ttttatttca cttcacgcca
     7021 attccttcag tgatgaaaat gtcggtgggc ttgaggtgta tcaccttgat aacacaagag
     7081 ataactatac agataaactt gcaatggtcg aaaacaagat aagcgattct tcgtcgctgc
     7141 tcaacactat tctcgtagac atgacaatga gctattttgt gaaagacagt ctgaattatg
     7201 ccacttctat aggaatcgga ctgaaagaga accttaaaaa acacaatgtt aaagtccgcg
     7261 gctacaaaaa aggggcgctt ttttatgttc tggtaggagc gaggatgcct tctttactgc
     7321 ttgagatcgg gtatcttaca aataaaatgg aaagaggatt actgctcaaa gacaactatc
     7381 ttgatgatat tgctgtttca gtgcttgatg cgatcgcaaa gaccgcaaat gagaaaaaat
     7441 aatttttgcg aggggtgtgt catgaaaagg tttttaacag gttttttcca gattctgctt
     7501 gtttttgttt tcattgtcag ttgcggtgaa aacaaaacaa acgaaaaaga tgacgataat
     7561 gttactgaca atgaagtgtc tgataacgaa gataccgata atgaatcaaa cgacgagtgg
     7621 gagctttttg atgagccggt tcttaatccg tcaggcaaca ttcctctttc cgcatcgata
     7681 agtacatggc tgggcggagg tcaaacctct gtttctgttg cagttaaaga tatcgacgga
     7741 gtgtctaagg attttgaaaa agagtattca ctcgtcggat tgagtgatag agcggaaatt
     7801 cctgtacttg gtcttttccc cgaccatgaa aacattgttg taataaaagc atttgacaaa
     7861 gataaaaaac ttgttgctga aagagagttc aagatcaaaa cggctccgct tccgaagaat
     7921 ttccctgaag ttgaacttac tggaaagatc gaaagcggct ggacaattgt gaactggctt
     7981 tttacaccga gagaaagacc cgaaatgagt gcgatcgcag ttgatcagtc gggaaggata
     8041 aggtggtaca ccgattttcc ttatgcggcg gctttcccga taactgttca tgacggatat
     8101 ctttacactt cggatggagc aagcattctc tataaatatg attttatggg taatgaaatt
     8161 gaaaaatggg atgttgagcc gcacggatat actgaaatac accacgaaat aattataaag
     8221 gatgacggca acataattct cggtgtcagc aagaaggatg atccatggat tgaagatcgt
     8281 gtaatagaga taaatccgga aaatgatcag ttgagaggaa catgggattt aagaaaaaca
     8341 tttcctgatg tgtgcgatct tttttatgat gttccgctta cggaaggaga cgacccggca
     8401 gggcagaaaa acgatcctgt acacaacaat gcgatatggt ataacgctga agatgattcg
     8461 ataattctcg gctctcaaag atccggtgtt gccaagataa cccattcagg atatctgaaa
     8521 tggttcctcg cacctcacat tgttgcagtt attgatgatg aggacagtga tggattcagc
     8581 gattctctga tcgatggtta tgatgaagct gatccgacaa cccatatcgg tgatttcaaa
     8641 ggcgacaaat atgttataga cagaatgcct gttgccggaa aaccgcacga agtttacagt
     8701 tcttttgatt tcagatatca ggaatttctt cttacgccgg ttgataaaga cggtgatgag
     8761 ataacagatg tcgaagttct caacggattt aaggatcatg aagattttgc gtggccgttc
     8821 agacagcaca caccgactct gcttaaaaac ggaaatctta tggtgtttga caacgggctg
     8881 gcaagaaatt tcggctatcc gcccatttat tcaactcatt acagcagagc tgtcgaatat
     8941 gagatcacac ctgataaaga ggacggttac ggcggaacgg ttaagcaggt atgggaatat
     9001 gttcttcaaa atgatccgat gtggtactct ctgagcatag ttgtaagcgg agcaaatgag
     9061 atcgagaacg gcaaccggct tataacatcg ggatcgatcg gctcatcgtt ctttcctgat
     9121 cagctcaagg tgatgtacgg tgacggaccg gtcggagcac taattgtcga agttgatccc
     9181 aaagacaaca ccgaacttaa tcagctgttc ttcaaaagat atatcgatga cgaatatccg
     9241 aatactcagt tttcagcata cagggcatac agattcgagc tttctgcaaa attaaggtag
     9301 atatctacag ttcatactgc ttatagaact cttcgggctg aggcccttca tcatatttcc
     9361 ggagccattc ctgatatttt tcagtgtcat ttagaagaat gaccgtgtct ttcatctgag
     9421 ctttaaattt actgaatctt gttccgtcaa gctgcgggga ggcgatcatc tttattttca
     9481 gcggattaag ccattcacct ttcggacttt ttattccgaa atgaagatga ggaccggtta
     9541 ctcttccagt tcttcccatt gttccgatct tctgtctggg accgacagtt gagcccgccc
     9601 tgacataaaa gctgttaagg tggaggtagt aagtctggga tctgtctgca tgctgaatag
     9661 ttatctgttt tccgccgtaa tcggttgttt tgacatcaat gactttaccg ggagccacag
     9721 cgtaaatagg atctccgaca ggtccgccgt aatcaactcc gttatggaac gctcttctac
     9781 cggtcacagg atgtcttctc attccgaaag ggcttgttat gtgaactctg tcaacgggaa
     9841 gccgcatcgc actggggatg agcgcttttc cttcttttgt gtaatgagca ttgaaagctg
     9901 atttcggatc tgtgtcttcg aatctgaacg cttcgttgag tcctgccctt ttcccttcgt
     9961 atgaagcata aagtattttt gaacccggca ccatctgatc attgtaaaat ttatcttcaa
    10021 caagtattct gtatctgtca ccttttcttg catcagttct gaagcttact atgcattcga
    10081 gtattccgtt cgtcactccg cggatcgttc tggtgacatc ttctctatct ataagtgcct
    10141 gatttaatgt tgtttctatt tctccttcga taatgcggta ccttttttct gtcggcatta
    10201 tcttctgggt atatttaagt tttctgtcgg caggatctct ttttaaaatg tggaaagtga
    10261 tgatgtcggg atagaatacg aactcttcaa cgacaccttc attagaaagc ttcaccttga
    10321 aatcttcgcc tgcctttatc tggcttaagt caacatcgta tcttatagcg ttgataatct
    10381 ccatcgtgtg ggaaagctga atcccgggga gcttgagaag tgcctcctca aacccttttt
    10441 ttggaagtat cttgccgtgg atgaaccctt cagcatcaat ttcagaattt ttatcttttt
    10501 ctatcggttc atcagggaca gcagcatcac cctcttcatc acccttcttt cctggctcta
    10561 ctgatgccgg tgctgtctct gcaacaggtt ctgcaacagg ttctgcaaca ggttcgttga
    10621 tctttgccgt ttcagcagtg ggggaactct cttcaacttt ttcaggaatg acagtatttt
    10681 cttcaactgc actttcttca acttgcggag tgtcgtggaa gacaacaagg aggacaagaa
    10741 gaaccgctac agcaataagg atgaagaata ttctgttctg accacggttt ttcatcaaaa
    10801 tcgaaactgc cgctcctgat tagaaaaaac aacggcaaaa cctaacagaa aagggtgtgg
    10861 cagtcaatac aaaaattaaa attactgatt ttaaagaatt tcttccggta atatttctgt
    10921 gtcgattgct tcgttccttg tttcaggata gtcgatgttg aagtggagtc ctcggctttc
    10981 cttccgtttc attgcacttt ttatgatgat ctcggctaca agtgtgatgt tcctgagctc
    11041 aagaagatct cttgttatct tgaaattcca gtagtattca ttgatctcat gtttaataag
    11101 ttcaagccgt ttgagcgccc gctcaagccg cttatcgctt cttacgatcc cgacataatt
    11161 ccacatgaac cttcttatct catcccagtt ttgttttata atgatctttt catcgatgct
    11221 tcctgccgat tcatcgttcc agtcaggcgg ctcctgaacg atctttgtcg atgatccaag
    11281 gtattgcagg atctcttttc ccgccgcatg agccatgacc gccgcttcaa gaagtgaatt
    11341 tgatgccagc cggttggctc cgtgaagacc tgtgtgggcg atctcaccgc taataaaaag
    11401 accgggcaga gaagtttttc ccgaaacatc gcttttaagt ccgccgcaca tgtagtgggc
    11461 agcagggaca acgggaatcg gctgagttct catatctatc ccggcatcaa ggcagacacg
    11521 gaaaatgttg gggaatcttt tccttaaaaa atttccgtca agttttgtca tatccagaaa
    11581 tacacagtcg tcaccgcttt ttttcatttc aaaatctatc gcccttgcaa cgatgtccct
    11641 tggggcaaga gatttaagag aatgatgttt ttccatgaaa gaatgtcccc gtttgtcgac
    11701 aaggacacca ccttctccgc gcaaagcttc agaaatgagc attgaagtca ctttttcgtg
    11761 atagagaatg gtaggatgga actgtatcat ctccatattt acagcggaaa ctcccgccct
    11821 gaagccgatc gccaccccgt cacccgttgt cacatcaggg tttgatgtat agagatatac
    11881 ttttccgcaa ccgcccgttg caaggagcac tgcttttgca attatggtaa taacattttc
    11941 tttatcgttc agaacatagg cgcctattat cctgttgtga tctgaaagca tgaacttgga
    12001 ggttgtgatc aggtcaacgg ctgtatgatt ttcaagcaca gttatttcag gatggttaag
    12061 gacacctttt tttattgctc tgatgagttc cattcctgtc atatcacctg cgtggacaac
    12121 ccttctttct gtgtggcccc cttcttttcc aagatcaagt gttccctgtt cgttctcagt
    12181 aaaaacacaa ccgagatcct tgaagtaatt tatggcatca gggccgtttt cgacaaaaaa
    12241 acggacgaca tcttctttgt tcagccctgc tccagccgaa atggtgtctt taatatgttt
    12301 ttcaaatgaa tcgtgaccga aatcgacaac acttgcaatt ccgccctgag catttgcgct
    12361 gtttgaaaca tctattgact cttttgtgac tatcagcact ttcgcattct tttcagcaag
    12421 acttaacgct gttgaaagac ctgaaagacc gcttccgata
//
LOCUS       DAOOYB010000068        12371 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_5332_length_12371_cov_9.697223, whole genome shotgun sequence.
ACCESSION   DAOOYB010000068 DAOOYB010000000
VERSION     DAOOYB010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12371
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5332_length_12371_cov_9.697223"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1059)
                     /locus_tag="PL056_05600"
     CDS             complement(<1..1059)
                     /locus_tag="PL056_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HPM46523.1"
                     /translation="MKKILHYILFAALMFLFSACDSGKDKIYGDDALSDSNAHVDDEM
                     TEYMERPDSDTYPGGGGTKTDPDNPVTDDVEETDDNPSVDVNDETPDETDEGIYSEDP
                     SVGTCESGRVAENQKTLVLDRLNYIRSLHDLPPVVYEEDDDVVTAECSLIIAANKQLS
                     HTPDNSWKCFSADAYTGCNKSNIFIQWGGDPLSFPSESVVDAFMTDENVESLGHRRWF
                     IDPWLAHISFGRVDDTYNKVLGSAIKVINDTEQSIASSSIEYVAYPYEYYPAELYNDN
                     VMMSFTLIYDRNNKWTNNKISFASSAIAITDPSNKTMKISGMKFDNDGYGVPNNIRWF
                     AEGIKSGVKYNVVITNVKV"
     gene            complement(1140..4328)
                     /locus_tag="PL056_05605"
     CDS             complement(1140..4328)
                     /locus_tag="PL056_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46524.1"
                     /translation="MLRKFFVLCFVVLSISVVSAQSKIAVLPAAGKGKSAQMAKNLAK
                     EIKSIYKTVKDVNLIDAERVLSASERSDFNACSTKQKCVADRSKGINKVDMILFPVVR
                     SKEGTFNVTLYIFSTKSGKRVAKPVVKGEEDIDAEDLAADIAAALIDASSNLTVETDE
                     TDGEDDEDDSGSSASAGKKEEVIEKVSPREQKNRLRAGFKAYKSGDAKGAAKLFREGG
                     DETLAGMAEDIDNAVQNAKKLIKDKDFGEAVKILNSVESRDFELREKGYKELQFIKET
                     NKKHKYNEPSDSDYTKVKNIFRDIKKDIKAVADWKNKEIEKLDDSMRGELKQKERITR
                     DFEKFEKEQREKEKNMESDHLKKIEKMKSDLENLDSKYRDKISDIEREISSLNKKLED
                     TRSPEEVYKTDIEGELKELDKKYKRLSLEQKKQLVMTQKQAKADIEKAEKDNEAKLKD
                     IEKANADLDKKIQELSKEIEKLNNDFDKNEQRESQQFEKGLAAQEAKDRKDREDLEKK
                     SSAEIEAMNKELEEYDKKIQAAAEKIDKLDREISDYVDKQDQRLQKVQENTDKKREEF
                     ERKFDADRQSAESKAEQEYQKGQSALARKIELLESSILKIEDKVDNYEKDPKWKNEKK
                     NLKSATDELIKYEDAHDAFINKQIAPVVNAHKENVAKLDASFKTLEGTIKKEIAAYKT
                     KKNAERSSLDKEVKSLEKGKPAFEKQLQAKIRTATANREKQMKAIDSRSAARDKERES
                     KSKARKTEFDKTVDAKRKQLAALEKQISVNTANADKQRAALSDQLEKTRIANEKKLSD
                     LEIANEKKREANEAAQEKERLAIYQKYEQKMAADRKEVNAKIAQFENNMRNLIAQRGK
                     EENTLRANIQNAEKQTDTMQSGWANEAKKRLAAYDANLKNAEKKEVAAKAKYEAALKS
                     IENQYKSKVDAIIQTASKKASSGGAGAEFTVERDRNFEFSSYTKSINATKADALSARG
                     MEKLKSDDIAGARKDFFEALYADTESKSALDGLKEIEKTAGAMYDKAYKMVTEDPDMA
                     KKILIDLRRNLSPKSEYYLKTLALLEELKAGE"
     gene            complement(4473..6557)
                     /locus_tag="PL056_05610"
     CDS             complement(4473..6557)
                     /locus_tag="PL056_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009244868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPM46525.1"
                     /translation="MNSYQNNSENGNQDPLKKYGRNLVELAKSGKLDPVIGRDSEIRR
                     IVRILSRRTKNNPVIIGSPGVGKTAIVEGLARRILRGDVPVSIQNKEIFELDMGALIS
                     GAKFRGEFEERLKEVLKAVKDSDGKIILFIDELHNIVGAGRAEGSMDAANMLKPMLAR
                     GELHCIGATTLDEYRKYIEKDAALERRFQPLIVQPPDEEETISILRGLRERFEIHHGI
                     TISEGAMVAAVKLSTRYISDRFQPDKSIDLIDEACSMLRTEIDSMPAELDDLKRKKMQ
                     LEIEMEGCKREQSGSTNEQVAAIDKKLREISKVYEEKFKIWENEKKAVETVKKLKKEI
                     EETKIEIERAEREYNLSRVSELKYGTLSDLQNKLKSAQEKTSSENVMVTEMLTEKQIA
                     EVVSKWTGIPVASMTQSEKDRILSLPEKLKECIIGQDEPIESISRSILRSKAGLTNPN
                     RPLGSFIFLGPTGVGKTELAKRTAEQLFDTSENMIRIDMSEYMEKHSVSRLIGAPPGY
                     IGYDEGGQLTEAVRRKPYSIILLDEIEKAHDDVFNILLQILEDGRLTDNQGRTVSFKN
                     TIIIMTSNIQKDLVKSKFKPEFLNRIDDIIEFKGLGHDELKKITQLLIADINKVLADR
                     EISFELDNSAMEKVIKESFDTEYGARPVRRYLEKKIETLLAVSILNGTVTAPSKSVVT
                     VEQGLFKLKTLK"
     gene            complement(6627..7892)
                     /locus_tag="PL056_05615"
     CDS             complement(6627..7892)
                     /locus_tag="PL056_05615"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HPM46526.1"
                     /translation="MRKSVIFLFVIFLCSSAVAAKPKIAVMDIHDRSNKMSKDLLENA
                     TEMIRGKLSATGQFMVIDRSSQQEKMKQMIGKEKKESYNQNYDKESQIPLGRALAADS
                     ILRSTISCLGNDCMLSAEIVDIAREVSTKGGTSKFIFDKSDLSSLIAAIEDVVEQMAD
                     MSKGELSSKPFPQDPGNDVFVDPNASFKIIFDASDQAEVYVDGARVCKSTPCQQYVGG
                     GMHQVKMVSRKMAPQEESIKFYRDERIIFNFAPAGAGVTITPVTVKGRTITDVSVYNG
                     RNVIGTLPGTLFLTPDNNKIVLKRAGFQDHKMKVNVTGNFDLNLSPIMEFEKYRPYKG
                     GAIAGFVVGGILFVAGGIGYLVAETMDALGEDADTAFIASYSLLGIGVGGIVLGAVLI
                     SIKRPVPGAEGFTFDVSGNGAKVSYTYKF"
     gene            complement(7925..8485)
                     /locus_tag="PL056_05620"
     CDS             complement(7925..8485)
                     /locus_tag="PL056_05620"
                     /inference="COORDINATES: protein motif:HMM:NF014961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HPM46527.1"
                     /translation="MDNGLNLFENIQFSDILAHLNKGVLFRSRWQLSVSEGEKELERI
                     LEEYHTGQLVIKGVCKIITNEKKGYFAVTVGSSGHEKALEIYKMDRYFDYFLFNGLLS
                     ELAEAAAEWIDQFIRLELGIDKSSRISPGYPLWPDITDQEKLLKMLTAEKIGISLTEA
                     YQLVPEHSITGMVVENKNLEKIKLNG"
     gene            complement(8478..11000)
                     /locus_tag="PL056_05625"
     CDS             complement(8478..11000)
                     /locus_tag="PL056_05625"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HPM46528.1"
                     /translation="MNNISKIIKERPLLFDGAMGTSIINFGLTADDYHGHINCNDYLS
                     ISRPDVITSIHEAYLESGCDIIETNSFGSNPLSFSEFGIADKAFETAKAAAMTAKKAA
                     LKFSTDSKPRFVSGSVGPGTKLPTLLQSDFDTLYNSYLIHFSALIEGGVDLFQIETCQ
                     DLLQIKSALAACYDAMKKYRCDLPVIVQVTVEKNGKMLLGTTLETVVTALSGFDLFAL
                     GLNCGTGPEGMYESIKTLKKISPFPVSVLPNAGFPELINGKLQYSLSAEKFGEHIGFF
                     VKDLGVEIVGGCCGTTPEHIRSAYGNINDFKMMAQKYPLAGSAVCSTFAVSELDVELK
                     PLIIGERANATGSKEFREALLSGDTDGMMDIISSQQSEGSHIIDLSVGYAGRNELEDM
                     AQLVKLINRDVPTPLCIDSTDPQTIETALKCYGGKALINSINFEDGGVKAEKILTLAK
                     KYGAAVIGLTIDETGLAETVERKMEVAERLINSVLKAGLHRSDLLIDTLTFTLGSGDP
                     KYFNAAVESFKAVKKIKELYPDVFTVLGVSNCSYGLKPKARKVLNSVYLFHAVKWGLD
                     ASIFHAGKVVSLDCVESSAKKICEDLIFNRRSDGYDPLHELINYFENDKLQDEKSSAA
                     ASLDPSEKLFSHVIEGKTKGLEETVSELLETTEPLEIINNILLSAMKQVGVRFQNGTT
                     QLPFVLKSAEVVKKACSIVEPHIKSGEVNKKGTIVLATVKGDIHDIGKNLVDIILSNN
                     GYTVHNIGTDRSAAEIAAAVDEIRPDAVGLSALLVKSALEMKNVAQFLSSNGMEIPLI
                     CGGAALTNDFVEKEVSPLNRKSAYYANDAFEAMNILEKLKNG"
     gene            complement(11016..>12371)
                     /locus_tag="PL056_05630"
     CDS             complement(11016..>12371)
                     /locus_tag="PL056_05630"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HPM46529.1"
                     /translation="ENTLPGGKTVALKTVAAIAHLSVRGFPVPCSFAKIPFFNEIFII
                     QGDNQSAVSGESSFSSHLKLLSETSRAVTEKSLVLIDEIGTGTDPLQGGAIARAYLEF
                     IAEKNCHAVVTSHLAEVKSIALENKKFIPVAMGFDTGSDKPTYKFSYNIVGSSNALSL
                     VKKIGFPAEFVTKLENLLFAKENSIEPLINRLRKKEEELDRLKEETEQLRTAAIIERE
                     NAAAIKISLEKKEKHFEASRLNSLKKLVEMEEMELKKKISAVDKKDAPVKVALIKKEK
                     ESIQDAINRQKVSLDEKKGEKLADVAGSIIPGKTIVYDKLLKIEGILQQVKNGKAEYI
                     CNGKMLVSPVERLIVLKMESGVKTSSSKNPVESKYAETCDVRGLFVEDAAEKIEKALD
                     NAFGGGAASLTIIHGHGSGKLKKFIRDTLPSFQKRYRFEFNSGTSEEGGDAVTVIRFS
                     K"
BASE COUNT         2997 a         2749 c         2333 g         4292 t
ORIGIN      
        1 tactttgaca ttggttataa cgacattata ttttacacct gattttatcc cttcggcaaa
       61 ccatctgata ttattgggaa ccccataccc gtcattatca aacttcatgc cggagatctt
      121 catcgtctta tttgacgggt cggttatggc tatcgcagaa gaagcaaaac ttatcttgtt
      181 gtttgtccat ttattattcc tgtcataaat gagcgtaaaa gacatcatca cattatcgtt
      241 gtaaagttct gccggatagt attcgtaagg ataggcaaca tattcaattg agcttgaagc
      301 gatgctctgt tcagtatcgt ttattacttt gatcgcagat cccagcacct tgttgtaggt
      361 atcatcaaca cgcccgaaag aaatatgggc aagccagggg tcaatgaacc accttctgtg
      421 accgagcgat tcaacatttt catcggtcat gaaagcatct acaacggatt cacttggaaa
      481 agagagcgga tctccgcccc attgaatgaa aatattgctt ttattgcacc cggtataggc
      541 atctgcagaa aaacatttcc aactgttatc cggagtgtgg ctcaactgtt tattggcagc
      601 aataatgagg gagcattccg cagtaacaac atcatcatct tcttcataca ctacaggcgg
      661 cagatcgtgc agtgatctga tgtaattaag ccggtcaaga accagagtct tttggttttc
      721 tgcaacccgt cccgattcgc atgttcctac agaagggtct tcagagtaaa ttccttcatc
      781 agtttcatca ggcgtttcat cgttgacatc aaccgaagga ttgtcatcag tttcttcaac
      841 atcgtctgtg acgggattgt caggatcggt ttttgttcct ccgccgccag gatatgtgtc
      901 actgtccggg cgttccatat attcggtcat ttcatcgtca acatgtgcat ttgagtcact
      961 cagagcatca tctccgtata ttttgtcttt tccgctgtca caggcagaaa ataaaaacat
     1021 aagagccgca aataaaatat aatggagaat ttttttcata ttgtacctct tgttaaaaaa
     1081 aacttcatta ttatatcatc tattgaatca aaagaaaagg ggagcggtat tatgatattt
     1141 tattcgccgg ctttcaattc ttcaagaaga gcaagagttt taaggtaata ttcacttttc
     1201 ggactcagat tccttctaag atcaataagg atcttcttag ccatgtcagg atcttcggtc
     1261 accattttat atgccttgtc atacattgcg ccggcggttt tttcgatctc tttcaatccg
     1321 tcaagagctg atttgctttc cgtatcagca taaagtgcct caaagaaatc ttttcttgct
     1381 cctgcaatat catctgattt gagtttttcc attcctcttg cagaaagggc atctgcttta
     1441 gttgcgttaa tggattttgt ataactgctg aattcgaagt tgcgatctct ttcaactgta
     1501 aactctgctc ctgctccacc tgatgaagct ttctttgatg cagtctgaat gatggcatca
     1561 actttagatt tatattgatt ttcaatgctc tttagagctg cttcatattt tgcttttgcg
     1621 gcaacttcct tcttttcagc atttttcagg tttgcatcat aagcggcaag tctctttttt
     1681 gcctcgttag cccagccgct ctgcatagta tcagtctgtt tttcagcatt ctgaatgttt
     1741 gctcttaatg tgttttcttc cttgcctctc tgggctatga ggtttctcat attgttttca
     1801 aattgagcta tttttgcatt gacttctttt ctgtcagccg ccattttctg ttcatatttc
     1861 tgatatattg caagccgctc cttttcctga gccgcttcat ttgcttctct tttcttttca
     1921 tttgcaattt caaggtcgct gagtttcttt tcgttggcga tccttgtttt ttcaagctga
     1981 tctgaaagag cggctctctg cttatctgca tttgccgtat ttaccgaaat ctgtttttca
     2041 agtgcggcaa gctgttttct ctttgcatcg acagttttgt caaattcagt ttttctcgcc
     2101 ttcgattttg attcgcgttc tttgtctctt gcggcacttc tgctgtctat cgccttcatc
     2161 tgcttttctc tgtttgcagt agcggttctg atctttgcct gaagctgttt ttcaaatgcg
     2221 ggctttcctt tttcaagaga ttttacttct ttgtcaagag aacttctttc agcattcttt
     2281 ttagttttat aagcggcgat ctcttttttt atggtccctt caagagtttt gaaagaagca
     2341 tccagtttag caacattttc tttatgtgca ttaacaaccg gagcgatctg cttatttatg
     2401 aatgcatcgt gtgcatcttc gtattttatc agttcatctg tggcagattt cagatttttc
     2461 ttttcatttt tccatttagg atccttttca tagttatcaa ccttatcttc tattttgaga
     2521 atactggatt caagcagctc gatctttctt gcaagagcag actgaccttt ctgatattcc
     2581 tgttcggctt tggattcagc ggattgtcta tcggcatcaa attttctttc gaactcctct
     2641 cttttcttat ctgtgttttc ctgaaccttc tgaagccgct gatcctgctt atcaacataa
     2701 tcgcttattt cgcggtcaag cttatctatt ttttccgctg cggcctggat tttcttgtca
     2761 tattcctcaa gctctttgtt cattgcttct atttcagcag aactcttttt ctcaaggtct
     2821 tcacgatctt ttctgtcttt ggcttcctgt gcggcaagtc ctttttcaaa ctgctgactt
     2881 tcgcgctgtt cgttcttgtc aaaatcgttg ttgagttttt cgatctcttt tgaaagttcc
     2941 tgtatctttt tgtcaagatc ggcattcgct ttttcgatgt ccttgagctt tgcctcgtta
     3001 tctttttcgg ctttttcaat atcagctttt gcctgtttct gagtcatgac gagctgcttt
     3061 ttctgctcaa gagaaagtct tttgtatttt ttatcgagct cttttaattc accctcgata
     3121 tctgttttgt aaacttcctc aggtgagcgg gtgtcttcca gtttcttatt cagcgaagat
     3181 atctctcttt ctatatcaga gatcttgtca cgatattttg aatcaagatt ttcaaggtct
     3241 gatttcatct tttcgatctt tttcagatga tcagattcca tattcttttc tttttcacgc
     3301 tgttctttct caaatttttc aaaatctctt gttatccgtt ctttctgctt gagttctccg
     3361 cgcatcgaat catcaagttt ttcgatctct ttgtttttcc agtctgcaac tgctttgatg
     3421 tcctttttta tatctctgaa aatgtttttt acttttgtgt aatcagagtc actcggctca
     3481 ttatatttat gttttttgtt tgtttccttt atgaactgaa gctccttgta acctttttca
     3541 cgcagttcaa aatccctgct ttcaacgctg ttcaatatct ttacagcttc tccgaaatcc
     3601 ttgtctttaa taagtttttt tgcattttgc actgcattgt cgatatcttc cgccatccct
     3661 gcaagagttt cgtctccccc ttccctgaaa agttttgccg caccttttgc atcaccgctt
     3721 ttgtaagctt tgaatcctgc acggagacgg ttcttctgtt ctctcggaga tactttttct
     3781 attacttcct cttttttccc ggcacttgct gaagatcctg aatcatcttc gtcatcttcg
     3841 ccatcagttt catcagtttc aacagtaaga tttgaagatg catctatcaa tgcggctgca
     3901 atgtcggctg caaggtcttc ggcatctata tcctcttcac cctttacgac tggttttgca
     3961 actcttttgc ctgattttgt tgagaaaata tagagcgtta cattgaatgt tccttctttg
     4021 ctccttacaa cggggaaaag tatcatgtca actttgttta tcccttttga tctgtctgca
     4081 acacatttct gttttgttga gcaggcattg aaatcgcttc tttcacttgc agataaaact
     4141 ctttctgcat caataagatt gacatctttt actgttttgt agatggattt gatctctttt
     4201 gcgagatttt tcgccatctg ggcggatttc ccttttcctg ctgcgggaag aacagcaatc
     4261 tttgactgag cggaaacaac cgaaatagag aggacaacga agcataaaac aaagaatttt
     4321 cttaacattt tcacaccttt cttattacaa acatattaaa atgaacagtg aaaagatcac
     4381 tacaactgct ttcatgaaac cacaaattca cgacagagta ataaaaagga ccttttttgt
     4441 caaaattttt attggatttt aaaatacagg tattatttaa gggttttaag tttaaatagt
     4501 ccttgttcga cagttacgac agatttagaa ggagccgtaa cagttccgtt caggatagaa
     4561 acagcaagca gtgtttcgat tttcttttcc agatatcttc tcaccggtct tgctccgtat
     4621 tcggtgtcaa aggattcttt tatcactttt tccattgcgg aattgtccag ttcaaaggag
     4681 atctcacgat ctgcaagcac tttattaata tctgctatca ggagttgagt tatctttttg
     4741 agctcatcat gtccaagccc tttgaattca atgatgtcgt ctatcctgtt taagaattca
     4801 ggtttaaact ttgatttgac aagatctttc tgaatgtttg atgtcatgat gatgatagtg
     4861 tttttaaatg aaaccgttct tccctgattg tcagtaagcc gtccatcttc aaggatctga
     4921 agcagaatat taaacacatc atcatgggct ttttcaattt catcaaggag aattattgaa
     4981 tacggttttc tccttacagc ttcggtaagt tgacctcctt cgtcataacc gatatatccc
     5041 ggaggtgctc ctatcagtct ggaaacactg tgcttttcca tatattcact catgtcgatc
     5101 cggatcatgt tctcagatgt gtcaaaaagc tgttcggcgg ttcttttggc gagctcagtt
     5161 tttcctacac cggtaggtcc cagaaaaatg aaagaaccaa gcggtctgtt cgggtttgta
     5221 agtcccgctt ttgatcttaa tattgagcgg gagatacttt ctatcggctc atcctgtcca
     5281 ataatacact cttttaattt ttccggcaga gaaaggatgc ggtccttttc gctttgagtc
     5341 atgctggcaa cgggaattcc agtccattta ctgacaactt cagcaatctg tttttcggta
     5401 agcatttccg tgaccataac attttccgag gatgtctttt cctgcgccga cttaagttta
     5461 ttctgaaggt cggacaaagt tccgtatttg agttcgctta ctctgctcag attatattcc
     5521 cgttcagccc tttctatttc gatttttgtc tcttcgatct cttttttcag ttttttcaca
     5581 gtttctacgg cttttttctc attttcccaa attttgaact tttcttcata tactttactt
     5641 atctcccgaa gttttttatc aattgcagca acctgttcat tagtggatcc gctctgttcc
     5701 cttttacagc cctccatttc gatctcaagc tgcatctttt ttcttttaag atcatcaagt
     5761 tcagccggca tgctgtctat ttcagttcta agcatcgaac atgcttcatc tataaggtct
     5821 attgatttgt cgggctgaaa tctgtcagaa atgtatcttg ttgaaagttt tactgcggca
     5881 accattgctc cttcagaaat tgttattccg tgatggatct caaatctttc tctgagacct
     5941 cttagaattg agatggtctc ttcttcatca ggcggctgga caatgagtgg ctgaaacctc
     6001 cgttcaaggg cggcgtcctt ttcaatatat tttcgatatt catcaagcgt tgttgctccg
     6061 atgcagtgca gttctcctct tgcaagcatc ggcttaagca tgtttgccgc atccatgctt
     6121 ccttcagctc ttccggctcc gacgatgttg tgaagttcgt caataaaaag gattatcttc
     6181 ccgtctgaat cttttacggc tttgagcact tccttgagcc gttcttcaaa ttctccgcga
     6241 aatttggcac ctgatatcaa agcacccatg tcaagctcaa atatctcctt attctgaatg
     6301 cttacaggca catctccacg caatatcctt cttgccagac cttcaactat tgctgttttt
     6361 cctacaccgg gggagccgat tatcacagga ttgttcttcg ttcttctgga aaggatcctc
     6421 acaattcttc ttatttcgct gtctcttcca ataacagggt cgagtttccc gctttttgca
     6481 agttcaacaa gattcctgcc gtattttttc aacggatcct ggttcccgtt ttcgctgtta
     6541 ttctgatagc tgttcataaa aaacctccct gaaaattacc ggataactta atagtgacag
     6601 ttttgattat ttcaagggat aggggattag aacttatatg tgtatgatac ctttgcgccg
     6661 tttcctgaaa catcgaaagt gaagccctca gcaccgggaa caggccgttt tattgagata
     6721 agaacagctc cgagcactat tccgcccact ccgattccca gcagtgaata gcttgcaata
     6781 aaagctgtat ctgcatcttc gcctaaagca tccatcgttt ccgcaaccag ataaccgatt
     6841 ccgccggcga caaaaaggat tccgccgaca acaaaacctg ctatggctcc gcctttgtag
     6901 ggtctgtatt tttcaaattc catgatcgga ctcaaattga gatcaaaatt tcctgttaca
     6961 ttcactttca ttttgtggtc ctgaaaaccg gctcttttca aaacgatctt gttgttatca
     7021 ggagtaagga aaagtgttcc ggggagagtc ccgatgacat tccttccgtt gtagactgaa
     7081 acatcggtaa tggttctccc ttttactgta acgggggtta tggttacgcc ggcaccggcg
     7141 ggagcgaaat tgaaaattat tctttcatcg cggtagaatt ttatggattc ttcctgaggg
     7201 gccatttttc tgctcaccat cttaacctga tgcattccgc cgcctacata ctgctggcag
     7261 ggtgtcgatt tgcagactct tgctccgtct acataaactt ccgcctggtc gctggcatca
     7321 aaaattatct tgaatgatgc gttgggatct acaaaaacat cattgccggg atcctgagga
     7381 aacggttttg atgaaagttc gcctttactc atatcagcca tctgttcaac gacatcttca
     7441 atagcggcga tgagagatga aaggtctgat ttgtcgaaaa tgaactttga agttccccct
     7501 tttgtagaaa cttctcttgc gatatcaact atttcggcgg aaagcataca gtcgttccca
     7561 aggcaggata tggtgcttct taatattgaa tctgcggcga gtgctctacc gagagggatt
     7621 tgagactctt tgtcgtagtt ctgattgtaa ctttcctttt tttccttgcc gatcatctgt
     7681 ttcatttttt cctgctggct cgacctgtca attaccatga actgacctgt tgcagaaagc
     7741 tttccacgga tcatttcggt cgcattttca agaaggtctt ttgacatttt gtttgaacgg
     7801 tcatgaatgt ccatgacagc gattttgggt tttgccgcaa cagctgatga acataaaaaa
     7861 atcacaaaaa gaaaaataac tgactttctc atattaactc ctcaatgaaa aatctcgcaa
     7921 tacattagcc gtttagtttg attttttcaa gatttttatt ttcaacaacc attccggtga
     7981 tgctgtgttc ggggacgagc tgataagctt ctgtgagcga tattccgatc ttttcggcag
     8041 tgagcatttt tagaagtttt tcctgatctg ttatgtcagg ccagagcgga tatcctggtg
     8101 atattcttga tgatttatca attccaagtt caaggcggat aaattgatct atccattctg
     8161 ctgcggcttc agcaagttca ctgagaagtc cgttgaaaag aaaatagtca aaatacctgt
     8221 ccattttgta tatttcaagt gccttttcat gccccgaact tccgactgtt actgcaaaat
     8281 atcctttctt ttcattagtg attattttgc agaccccttt tattacaagc tgcccggtgt
     8341 ggtattcctc taatatcctt tcaagttctt tttccccttc agaaacggat aactgccatc
     8401 ttgacctgaa aagaacacct ttgtttaagt gggcaagaat atcggaaaat tgaatgttct
     8461 caaataaatt taaaccatta tccattcttt agtttttcca gtatgttcat cgcttcaaat
     8521 gcatcgtttg cataataggc actttttctg ttaagagggg agacctcttt ttccacaaaa
     8581 tcatttgtca gcgccgctcc gccgcagatc agaggaatct ccattccgtt tgaagaaagg
     8641 aattgggcga catttttcat ttcaagagca cttttaacta gaagcgcact caatcccacc
     8701 gcatcaggcc tgatctcgtc aaccgccgcc gctatttctg ccgcagacct gtcagttccg
     8761 atattgtgaa cagtatatcc gttatttgaa agaatgatgt caaccagatt tttgccgatg
     8821 tcgtggatgt cacctttaac agttgcaaga acgatggtcc cttttttgtt aacctcaccc
     8881 gattttatgt gcggctcaac tatcgagcat gcctttttta ccacttcagc acttttcagc
     8941 acaaacggga gctgggtcgt tccgttctgg aaccttacac cgacctgttt catcgcagac
     9001 agaagaatat tgttaattat ttcaagtggc tcagtggttt caagaagctc gcttacagtt
     9061 tcttcaagcc cttttgtttt cccttcaatg acatgagaga aaagtttttc agaaggatca
     9121 agtgaagcgg cggcactgct tttttcatct tgaagcttat cgttttcaaa atagtttata
     9181 agttcgtgta acgggtcata tccatctgat ctacggttga atatcagatc ttcacaaatt
     9241 ttctttgcac tgctttcgac acagtcaaga gaaactactt tgccggcatg aaaaattgaa
     9301 gcgtcgagtc cccatttcac tgcatggaaa agataaacgg aattaagcac ttttctcgct
     9361 ttcggcttta aaccgtaaga acagttgctg accccgagaa ctgtaaaaac atcagggtaa
     9421 agctctttta ttttttttac agctttaaag ctttcaactg cggcattaaa atatttcgga
     9481 tcacccgaac ccaaagtgaa ggtaagggta tcaataagaa gatctgatct gtgcaaccct
     9541 gctttaagaa ctgaatttat caatctttct gcgacttcca ttttcctttc aactgtttca
     9601 gccaaccctg tttcatcaat tgtaagtcct atgaccgccg caccgtattt tttcgcaagt
     9661 gttaatattt tctcagcttt taccccacca tcttcaaaat taatgctgtt gataagagct
     9721 ttccctccgt aacatttaag agccgtttca attgtttgcg gatctgtcga atcaatgcaa
     9781 agaggtgtcg ggacatcacg gttgataagt tttacaagct gtgccatatc ttccaattca
     9841 ttccttccgg catatccgac tgaaagatcg attatgtgcg agccctctga ctgctgagat
     9901 gaaataatgt ccatcatccc gtcagtatca ccggaaagaa gagcttctct gaattccttt
     9961 gatcctgttg cgtttgccct ttcaccgatg atgagtggct ttagttccac atcaagttca
    10021 ctcactgcaa atgttgaaca tacagccgat ccagccagag gatatttttg agccatcatt
    10081 ttgaaatcgt tgatgtttcc gtaagcagat ctgatgtgct cgggagttgt tccgcaacag
    10141 ccgcctacaa tttccacacc gagatcttta acaaaaaaac cgatgtgttc tccgaatttt
    10201 tccgctgaaa gcgaatactg aagttttccg ttgataagtt caggaaatcc tgcatttgga
    10261 agaactgaaa cagggaaggg ggatatcttt ttaagtgttt ttatgctttc atacattcct
    10321 tcagggcctg ttccgcagtt gagcccgaga gcaaaaagat caaatcctga aagagcggtt
    10381 acaacagttt caagagttgt gccaagaagc atcttgccat tcttttcaac tgtcacctga
    10441 actataacgg gcagatcaca ccgatatttt ttcattgcgt cgtaacaggc ggcaagtgcc
    10501 gattttatct ggagaaggtc ctgacatgtc tcaatctgga agagatctac tcccccttcg
    10561 ataagtgctg aaaaatgaat gagatatgaa ttatagagtg tgtcaaaatc tgattgaaga
    10621 agagtcggaa gctttgttcc gggaccgact gaaccggaaa caaaccgcgg ctttgaatca
    10681 gttgaaaact ttaatgcggc tttttttgct gtcatggcgg cggcttttgc cgtttcaaat
    10741 gccttgtcag cgataccgaa ctcggaaaat gaaaggggat tcgatccgaa agagttggtc
    10801 tcaattatat cgcagccgga ctcaagatat gcctcgtgaa tggaagttat gacatccgga
    10861 cggcttatcg aaaggtagtc attacagttg atatgtccgt gataatcatc ggcagtcaac
    10921 ccgaaattaa taatggatgt tcccattgct ccgtcaaaaa gcaacggtcg ctcttttatt
    10981 atttttgaaa tattgttcaa tttaacacct cggatctatt ttgaaaatct gatcactgta
    11041 actgcgtcac cgccttcttc actggtccct gaattgaatt caaaacggta tcttttctgg
    11101 aaggagggga gtgtgtcgcg gatgaatttt ttaagtttcc ctgaaccgtg accgtgaatt
    11161 attgtcagag acgcggctcc gcctccgaaa gcattgtcaa gcgctttttc gatcttttca
    11221 gcggcatctt caacaaacag acctctcaca tcgcatgttt cggcatactt tgattcaaca
    11281 gggttctttg atgatgatgt cttaaccccg gattccattt tcaggacgat cagccgttca
    11341 acaggggaaa cgagcatttt accattacag atatattctg cttttccgtt ttttacctgc
    11401 tgaagtatcc cttctatctt gagaagtttg tcataaacta tcgttttgcc ggggattatc
    11461 gaccctgcga catctgcaag tttctcacct tttttttcat caagagaaac tttctgcctg
    11521 tttatggcat cctgaatgct ctctttttcc tttttgatca gtgccacttt cacaggggca
    11581 tcttttttat caacagcaga gattttcttt ttcaattcca tctcctccat ttcgacaagt
    11641 ttctttaatg aatttagccg acttgcctca aaatgttttt cttttttctc aagacttatc
    11701 tttattgcgg cggcattttc cctttcaata attgcggcag tgcggagctg ttcagtttct
    11761 tctttaagcc ggtcaagctc ctcttctttt ttcctgaggc ggtttattaa tggctcgatg
    11821 ctgttctctt ttgcgaaaag aaggttttca agctttgtaa caaattctgc agggaaaccg
    11881 atctttttta ctaacgacaa agcgttactg ctgccgacga tgttataaga aaatttgtat
    11941 gtcggtttat cagatccggt atcaaaaccc attgcgacag gaatgaactt tttgttttca
    12001 agtgcgatgg acttaacttc ggcaagatgg cttgtcacga cggcatggca gttcttttcc
    12061 gcaatgaatt caagatatgc ccttgctatc gctccgccct gaagaggatc agtacctgtt
    12121 cctatttcgt ctatcagcac aagacttttt tcagtgactg cccttgatgt ttcggaaagc
    12181 agtttcaaat ggcttgagaa acttgattca cccgaaacag cactctggtt gtcaccctga
    12241 attataaaga tttcattaaa aaacgggatc ttagcaaaag agcaggggac tggaaagccc
    12301 ctgacagaaa gatgagctat tgcagccaca gtctttaatg ccactgtttt acctccgggc
    12361 aaggtgttct c
//
LOCUS       DAOOYB010000069        12317 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_5363_length_12317_cov_7.679416, whole genome shotgun sequence.
ACCESSION   DAOOYB010000069 DAOOYB010000000
VERSION     DAOOYB010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12317
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5363_length_12317_cov_7.679416"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            26..2617
                     /locus_tag="PL056_05635"
     CDS             26..2617
                     /locus_tag="PL056_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46530.1"
                     /translation="MKKFLLLTLLFLLVSCDSGASKIINKDNDDESSNDSDVVVACTE
                     GKTKCVDNTVFKCESEEWVEWEDCSVQGLVCAVKAGAAQCLTDGGDTGNTGNTGNSGN
                     SGDSGDSGDSGDSGDSGNTGNTGNTGDTGNSGDSGDSGDSGNSGNSGDSGDSGDTGDT
                     GNTGDTGNSGNTGDSGDSGNSGDSGNSGNTGNSGDSGSGCIAYVDTRCNADDNVETCN
                     AGGDGWVLTSVCNDDCLNGKCIVNPSCTPNERRCVNKTAQKCNADGLAWIVTDVCKYG
                     CEAGECTGVCDTGEKRCNISGNAETCDSEGNWDVTGACTSTTPCIDEIGECAIESLSL
                     VGKTITMGGLYYYYGNVSLTASTTITVDPSIGWLEIHAGGNITIDATSGIYADVLKCG
                     PSDGGSASGYNYGGGGGGGHKDAGGNSKKYTSSTINVSGGAAVESLSILKYKTGSCGG
                     SNVYNSAGGKGGGAIILYGANITVEGTLSAKGGNGISEYYGGSGGGSGGSITLFGGMV
                     DLKSSADLNVDGGTGGTSGYSSNYHGGAGSKGMIKILSGQKSAGIIDPLAKVGSYNVA
                     TVAPPDKMSSTTHPDQTKWYNDGFSNILFSWNRPTYLAVDAYFYKINANSVFVPKSGD
                     GTYIEDNAVNIETGWINGTNYFHLTTNDIDAVSGTVSNSFVVNVNNTPPTITSSSHSN
                     TNTFYDVSTVALNWTDPTGKEGSFPAYRYIWDRYAQTTPSSTTGVLTDKKDLTRTGVP
                     AGIWYLHVISVDSMGYPTKTARHFKVNVGLQPGKGNIAGYVKVNGTNTEIQGATVKVN
                     DGIWNATTASNGSYSIGESTVYSNTVYSWPKWEVSAEAPGYKRSVQSIDVAVGETTPL
                     NFFLDPE"
     gene            2810..3673
                     /locus_tag="PL056_05640"
     CDS             2810..3673
                     /locus_tag="PL056_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46531.1"
                     /translation="MKHSRLSIFTIVFFVFLSFSLLGDDLFDLDGGSGGEAPAEETKP
                     AEETGAPEQGAETKDTPKTAQLEIGTAAADSGAEKELRDLNIEKKKKIKVVQKKDFHK
                     DGRFEMGIDLGFVNGDWEDVLVVGARMAYHINEYLAVQGRFLYGAVSWENETLKQADE
                     ADAYVAKSNMVMAGGLGVVFTPFYGKLSLFGDMIPKYDINITAGGYYYQTKSEDKNNS
                     VKPLTNHNGAFEIGFAPRIFLGKNASLSFNCDWFMMKDKRPKQYGGHSSYLKTDFMFT
                     VNVSMFLPFAK"
     gene            3688..5310
                     /locus_tag="PL056_05645"
     CDS             3688..5310
                     /locus_tag="PL056_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46532.1"
                     /translation="MKKTIILISITLSLLLVASCAAQLTVREKEMKKYEKIDPKLVEG
                     MPEAIVKVVKAYNDEDYFTSAVGFYAIMKNKEWDRLHETARYYYAESLFRMGLYQASE
                     YQLAEILFEGPDSHYFISSLLKLLAVTYETQDERVLFAVLSNVDYSVLPKKFANELTY
                     YLGKISFYNGQDDQAIKMFSGVKDYSSFYPKAKYFLGVIQVRQQMYAEAMKSFTEIKQ
                     MPDDKYLGGDIKKLKGMAELALGELYYAAAWQAQNKLAMFNVALNYFSNVGRDNAQWF
                     ESLFARTWASLMIGRFDSTLGTVVTLRSPFFTDIYFPEVNIVEAVTYYNLCQYDEVNN
                     VIDYFFGVYPDYSQKMTSWLENVSTKTGLDIYKELLKMYRDAQEGKKTELPEAVLKNI
                     LTESLFQRKYSHIKEIERELSIIENSPEAFKKSVIAEDVTKKMMYQLTTLRNESGIWL
                     VQRVQNLNGELAQLIADMRAIRFEMTDSQKAALEKEELHGKDIKEDEGQKQQSGMNPS
                     VPDGYYYFPFDGEYWEDELGYYFFNIENLCKE"
     gene            5383..8946
                     /locus_tag="PL056_05650"
     CDS             5383..8946
                     /locus_tag="PL056_05650"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPM46533.1"
                     /translation="MILAFSTSVFAQRKKRDSDTTTGPAKTKIETAEVKTDSKSMEKR
                     DEAIKQLNELIKDYPEGPRKAEVYRRLAELYWEKARGIKAVIMDEHNKKIDKYYEMND
                     PNAPMPELDLAASWEWNNKAIDVCDYVIKKYPDFQNIDEVYFFIASNLMEVGQPLRAI
                     RYYTLVVEKYTKSKFVPDAYFEMGEYFFNNNNVFKAIPSYKAIIDKFPENKFYGFALY
                     KYAWCMYNVGEYEQSVKLFQQVVINAEKIKDIALKEDALNDMVAPYAESGTVDDAEKY
                     FKTIVKEQKYFIAVLKRLADIYFDQDRSAEAIKIYKKLQQEAPARPEGPLWQKQIVEC
                     YKKLNNKELVREEIINLVKKYADKDAAWVKANAKDETTLESARQTAEAALRILTVDYH
                     NEARKTKGEETWKIVGELYPTYLKYFPKSEASYDMRFNYAEFLYDHKKYSEAGDQYQT
                     VAEMNPKGHHFEDASYGAVSCFGALLEAEHKKAQNEAKNRVAEAKKQGAGAIQANMVK
                     TADDKDAGKKDEVAAEFKPKPIPELHQKYINACDTYIKNIPRSKYLVDIIYQQAITYY
                     AFNHFDKAVPVFEMIVQKYPRHDLAVYSADLIMDSLNMSKDWELINNKSREFLRNSAL
                     LSGRNRLKTDLDNFKEMATFYSADIPHKNGKSLESADRYMAFATEFPKSKFNDVALYN
                     AVVYYQKGGDLYKSIRVQEQFLRESDDLYKKSNLKDKVMFGLAKNYEAIAYYDKAAQL
                     YIEFVEKSPTSKDAGDAVYNAAVLFESIGETEKAIDNYRLFVDKYAKTDKEKFQIALQ
                     YGYIWMRKGKTFYDRADKGFDRFLTDHLDIDGVKDYYYIDEACKKKPVSEVNKIKVEK
                     GDPNTILALYGAKKKMAREMGNTKEFYKNVDKVLQIFRGGEFKEKEDLNEISRDIIAE
                     ALLEEILPEFNEYMALKFDNIKVAPKHKGYEILQYGFDVEDGKVMHHGDGDVSQLAPS
                     DIANLKAAAKIKDDFNKESQEKMTKKIELTISLADKYAQIIEIAKSPRFTPASLFYIG
                     KIYKDLTDQMFNAPLAPWLSEAQIAEYKNFLDEKAFGAQKNAVAFFESAMKKGYETSV
                     YNDWVSKAKYELRFFQEVTGGKYYDENEIVPTPNMVETSSLIGKIDIDFKFPKITDEE
                     RKELKKMLNKPKTATTQSSAVAAPAAETVTPAEPTNEKQGE"
     gene            8950..10104
                     /locus_tag="PL056_05655"
     CDS             8950..10104
                     /locus_tag="PL056_05655"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPM46534.1"
                     /translation="MKKIILLITIIVSLVFVSCASAPDKKEEVKDDKKVIVVTQEPKE
                     PEDWAKYYTQKGMEALELKDGKADFDKAVEYFELATTAYPSAPIVHYNLGLVYQRKGD
                     DQNAVKHYNKALELDAKYSMPIINKAAIYANKLEYNRAIDVCEKFLEENDKDKGVLSA
                     VAGFYLLKGDYSNAIQSVRKILIQDQANVDALKNLGIFYFKKNDMGLASMVTTNSYNL
                     KPDDPGIANNRGVLYSTQKDDSLAMLYFSKAISNDPKNKSANFNVGSIAMKYGDAETA
                     YERFAMVLSQDPDNLLARIGLAISLRGLGRFEDSEREYMAVLQKDPNNALATFNLAVL
                     HYDHMNKKVEAKRNFRKYLSLAESVSDDHIVHQYLKDAVNIRPVEKTEGQ"
     gene            10180..10446
                     /locus_tag="PL056_05660"
     CDS             10180..10446
                     /locus_tag="PL056_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46535.1"
                     /translation="MKKMVIMFVLLLAFGIVWAQDQDTDEQGRLIKYREKTVIDFEDV
                     MLEGQIKKPSGQFLIDRSKTKFNSLINLKKDFNKELVRSVDLLN"
     gene            10507..11898
                     /locus_tag="PL056_05665"
     CDS             10507..11898
                     /locus_tag="PL056_05665"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HPM46536.1"
                     /translation="MRKRLLYDLTPIPEVPVNKNVTGKLEPEIRVVYDKRILLDLQKI
                     KKSFKVGSENVADCVVDETYVPEQAYEFATVSGTQFSINLKENLKGVIYKGDEKTDLT
                     EYVAKKGNTVDIDESTLLIVEFGKISICAMKSQLEPFKSGGFLKQFDAVLGIIMLLSF
                     LIGGFIVNMIIETPPIEVDIFELDNRFAKLIVGEEQDVKVEELEEEKKVKLEKPKEGL
                     VEASQQRKSLGEEGRVGDKKSRVANAQGSARRGLDAKVAQSSGIMGALSSGAANFDRV
                     FGGGGLGAGMEKTLGSVTGLAGVDQFGSGGLGTRGFGTGGGGNALGIGGLGTRGRGGG
                     GGGKYGMAAGQIGRKGRSDITAGGGQAVIMGALDRSVIDAYIKRNLAKIRWCYEKELN
                     KDPKLFGRIVINFIISGTGTVSSSKVNRTTMGNQNVESCVADQIKKIRFPAPKGGGIV
                     IVNYPFVFKHSEG"
     gene            11908..>12317
                     /locus_tag="PL056_05670"
     CDS             11908..>12317
                     /locus_tag="PL056_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46537.1"
                     /translation="MKKLLVIAAILLLSCSIFAESAEETGDVSGNDLNPVIRGFNFQV
                     SGGILAYVGKAGGENAGSPLGTAFKFNIGYDIPIKNVVNIGVAASIGMLQFNTNPNDI
                     GLGTKYNKSPWVEDYSPLTAGLEIEVAWMITQRW"
BASE COUNT         4052 a         2079 c         2832 g         3354 t
ORIGIN      
        1 ttaatgtttt ttgtgaggtt aaaaaatgaa aaagtttctg ttacttacgc tgttgttcct
       61 tttggtttcc tgtgattcag gagcatcaaa aataatcaac aaggacaacg atgatgagag
      121 ttcaaacgac agtgatgttg ttgttgcttg cacagaaggt aagacaaaat gtgttgacaa
      181 tactgttttc aagtgtgaaa gcgaagagtg ggtggaatgg gaggactgtt ctgtccaggg
      241 tctggtttgt gccgtgaaag caggtgcagc gcaatgtctc actgatggcg gtgatactgg
      301 aaatacaggg aatacaggta attcaggaaa tagtggtgat tccggagaca gcggtgattc
      361 cggagacagc ggtgattcag gcaacaccgg taacactgga aataccggag acacaggaaa
      421 tagtggtgat tctggagaca gtggtgattc aggaaatagc gggaattccg gagacagtgg
      481 tgattcagga gataccggag acacaggaaa taccggcgat acaggaaact ccgggaatac
      541 tggtgattca ggtgacagtg gtaactctgg tgacagcggc aactctggaa acaccggtaa
      601 ttccggtgac agcggttcag gatgtattgc ttatgttgat actagatgca acgctgatga
      661 caatgtggaa acatgcaatg caggtggtga cggctgggtt ctgacaagtg tatgcaacga
      721 tgactgtcta aacggaaaat gtatcgttaa tccaagttgc acgccgaatg agagaagatg
      781 tgtaaataaa acagcacaga aatgtaatgc cgacggactg gcatggatag ttactgatgt
      841 ctgcaaatat ggttgtgaag caggagaatg tactggagtc tgcgatacag gtgaaaaaag
      901 atgtaatata agcggtaatg cggaaacatg tgattcagaa gggaactggg atgttaccgg
      961 agcatgtacc tcaacaactc cttgcattga tgagataggt gaatgtgcga tcgaatcgtt
     1021 aagtctcgtc ggcaagacca taacaatggg cggactttat tattattacg gaaatgtatc
     1081 tctaaccgca tccacgacaa taacagttga tccttcgatt ggttggcttg agattcacgc
     1141 cggcggcaac ataacaattg atgcaacatc aggaatttat gccgatgttt taaagtgcgg
     1201 accttcagat gggggaagcg catcaggtta caattatggc ggcggtggcg gtggcggaca
     1261 taaagacgca ggaggaaatt caaaaaaata tacctcatca acaataaatg ttagcggtgg
     1321 agctgctgtc gagagtctta gcattttaaa atataaaaca ggatcatgcg gaggttccaa
     1381 tgtctataat tccgctggag gcaaaggcgg cggagcaatt atcctttatg gggccaacat
     1441 aacagtcgaa ggcactcttt ctgccaaagg tggtaacgga ataagtgaat attacggtgg
     1501 ttccggtggc ggatcaggcg gctctattac tctttttggc ggaatggtcg atctgaaatc
     1561 aagtgcagat ctcaatgtcg atggtggaac cggcggaaca agcggttaca gctcaaatta
     1621 tcacggagga gcaggaagca aagggatgat caagatcctt tccggacaga aatcagcagg
     1681 gataatagat cctttggcaa aagtagggtc ttacaatgtt gcgactgttg cgccacctga
     1741 taaaatgtct tcaacaactc acccggatca gacaaaatgg tacaatgacg gattcagcaa
     1801 catccttttc agttggaaca gacctacata tcttgcggta gacgcctatt tttataagat
     1861 caacgcaaat tccgtttttg tcccaaaaag cggagacgga acatacatag aagacaatgc
     1921 tgtaaacatc gaaaccggct ggattaacgg tacaaattat tttcacctga caaccaacga
     1981 tattgatgca gtatcaggaa ctgtttcaaa ttcatttgtt gttaatgtca ataacacccc
     2041 tccgaccata acatcttcgt ctcactccaa tacaaacaca ttctacgatg tttcaactgt
     2101 tgcactcaac tggaccgatc cgacaggtaa agaggggagt ttcccggcct acagatacat
     2161 ctgggacaga tatgctcaaa caacaccttc ttcgacaacc ggagttctga cagacaagaa
     2221 ggatcttaca agaacaggag ttcctgccgg tatctggtat ctccatgtga tcagtgttga
     2281 ctcaatggga tatccgacaa aaacagcccg tcacttcaaa gtgaatgtcg gtcttcagcc
     2341 cggtaaagga aatattgcag ggtatgtaaa ggttaatgga acaaataccg aaattcaagg
     2401 cgctacggta aaagttaatg acggtatctg gaatgcgaca acagcttcca acggttcata
     2461 ctccattggt gaaagcactg tttattcaaa tacagtttat tcatggccta aatgggaagt
     2521 ttcagcagag gcaccgggat acaaaagatc tgtccagtca attgatgtcg ctgtcggtga
     2581 aacaacacct ttgaactttt ttcttgatcc tgaataatga tctttcatag attctcaaaa
     2641 aaatgcattt aagaggtgga aaaattatta tcgcgaagat aattcaataa attcaatcat
     2701 atacatttgt ttcaatgatg gacaaaattt cttgacattg tttttaaaat aaaatagtct
     2761 tcgccgagag tttttggtgg attaacaatt cttttttagg taaggagcta tgaaacacag
     2821 cagattatcc atttttacaa ttgttttttt tgtttttctt tctttttccc ttctgggaga
     2881 tgatcttttt gatctcgacg gcggaagcgg aggtgaagca ccggctgaag aaacaaagcc
     2941 ggctgaagaa acaggtgctc cggaacaagg agcagagaca aaggacactc ctaagacggc
     3001 acagcttgaa ataggtactg ccgcggctga ttcaggagca gaaaaagagc tcagagacct
     3061 gaatatcgaa aagaaaaaga aaatcaaggt tgttcaaaag aaggatttcc acaaagacgg
     3121 tcgttttgaa atgggaatag accttggatt tgttaatggc gattgggagg atgttcttgt
     3181 cgtcggtgca agaatggcct atcacatcaa tgaatatctt gccgttcagg gtcgttttct
     3241 ctatggtgcc gtttcgtggg agaatgaaac acttaaacag gctgatgaag cagatgcata
     3301 tgttgcaaaa agcaatatgg tcatggctgg tggactcggt gtggttttca cccctttcta
     3361 cggcaagctc agtcttttcg gtgacatgat cccgaagtac gacataaata ttactgccgg
     3421 tggatattac tatcagacaa aatctgaaga taagaataac agtgttaagc cgctcacgaa
     3481 ccacaacggt gctttcgaga taggattcgc tccgagaata ttccttggaa agaatgcaag
     3541 cttgagcttt aactgtgact ggtttatgat gaaggacaaa cgcccgaaac agtatggcgg
     3601 ccactcttca taccttaaaa cagactttat gtttacggtt aatgtaagta tgtttcttcc
     3661 ctttgctaag tagtggggtg tcttaaaatg aagaaaacaa taatccttat ttcgatcacg
     3721 ctatcattgc tgttggtcgc atcatgtgcg gctcagctta cggttcgtga aaaggaaatg
     3781 aaaaaatatg aaaagatcga ccctaaactt gtcgaaggta tgccggaagc tatcgtaaag
     3841 gtggtcaagg catataacga cgaggattat tttacttcag cggtcggttt ttacgcgata
     3901 atgaagaaca aagagtggga cagacttcac gaaacggcta gatactatta tgctgaatca
     3961 cttttcagaa tgggtctata tcaggcatcg gaatatcagc ttgcagaaat actgtttgaa
     4021 ggccctgatt cacattattt catttcaagt cttttgaaac ttcttgctgt tacctatgaa
     4081 acgcaggatg aaagagttct gttcgcggtt ctttcaaatg ttgattactc agttcttcct
     4141 aaaaagtttg ccaatgagtt gacatattat cttggaaaga tcagcttcta taacggacag
     4201 gatgatcagg ccataaagat gttcagcggt gtgaaagatt acagttcttt ctatccgaaa
     4261 gccaaatatt tcctcggcgt tattcaggtt cgtcagcaga tgtatgcaga ggcaatgaaa
     4321 agttttacag agatcaaaca gatgccggat gacaaatacc tcggcggaga tattaaaaag
     4381 cttaaaggta tggcagagct tgcgctcggt gagctttatt atgctgctgc atggcaggca
     4441 caaaacaagc ttgcaatgtt caatgtcgca cttaattatt tctcaaatgt cggaagagac
     4501 aacgcccagt ggttcgaatc tctttttgca aggacatggg cttctttgat gataggaaga
     4561 tttgattcaa ctcttggaac tgttgtgact ttgaggtctc ctttcttcac agacatatat
     4621 ttcccggaag ttaacattgt tgaagcggtt acatattaca acctctgcca gtatgacgag
     4681 gtgaacaatg tcattgacta ctttttcggt gtttatcccg attactctca aaagatgacc
     4741 tcatggcttg agaatgtttc aactaaaaca ggactcgaca tttataaaga actcctgaaa
     4801 atgtatcgtg atgctcagga aggcaaaaag acagagcttc ccgaagcagt gttgaaaaac
     4861 atactcacag agtcactttt ccagagaaaa tattcacaca ttaaagaaat tgaaagggaa
     4921 ttgagtataa ttgaaaattc tcctgaagca tttaaaaaga gcgtaattgc cgaagatgtt
     4981 actaaaaaaa tgatgtacca gctaacgacg cttagaaacg aatcgggaat atggcttgta
     5041 cagagagttc aaaacctcaa tggcgagctt gcacaactta ttgccgacat gagagctatc
     5101 cgttttgaaa tgacagatag ccagaaagcg gctctggaaa aggaagagct ccatgggaaa
     5161 gatattaagg aagatgaagg tcagaaacaa cagtccggaa tgaatccttc tgttcctgac
     5221 ggctattatt atttcccgtt tgacggagaa tactgggaag atgaacttgg ttattatttc
     5281 ttcaacattg aaaatttgtg taaagaatag aatgttaact tcaaatagag gatccaacag
     5341 atgttaaaga gtaaatcatt gcgcgcctta ctggtgatct ttttgatttt agctttttca
     5401 acgagtgttt ttgctcaaag gaaaaaaagg gattctgata ctactacagg tcctgcaaaa
     5461 accaaaattg aaacagctga agttaaaact gacagtaagt caatggaaaa aagagatgaa
     5521 gctataaaac agcttaatga acttataaaa gactaccctg aaggacctag aaaagctgaa
     5581 gtttatagaa gacttgctga actttactgg gaaaaggcga ggggaataaa agctgtcatt
     5641 atggacgagc ataataaaaa gattgataaa tattatgaaa tgaatgaccc gaacgctccg
     5701 atgcccgaac ttgatcttgc cgcttcatgg gaatggaaca acaaggcgat tgatgtttgt
     5761 gattatgtta taaaaaaata tcctgatttc caaaatattg atgaagttta tttctttatt
     5821 gcttctaact tgatggaagt cggtcagccc ttaagagcaa taaggtatta cacacttgtt
     5881 gttgaaaaat ataccaaatc aaagttcgta cccgatgcct attttgaaat gggagaatat
     5941 tttttcaata acaacaatgt attcaaagcg attcccagct ataaagcgat cattgacaaa
     6001 tttcccgaaa ataagtttta tggttttgct ctctacaaat atgcatggtg catgtataat
     6061 gtcggagaat acgagcagtc agtaaaactt tttcagcagg ttgttattaa tgctgagaaa
     6121 attaaagaca ttgcgttgaa agaagatgct ctgaacgata tggttgctcc ttatgctgaa
     6181 tcaggaacgg ttgatgatgc cgaaaaatac tttaagacca ttgtaaaaga acagaaatat
     6241 ttcattgcgg ttttaaaaag actggctgat atctattttg atcaggatag atcggctgaa
     6301 gccataaaaa tttacaagaa actgcagcag gaagctccag caaggcctga aggtccttta
     6361 tggcagaaac aaatcgttga atgctataaa aaacttaata acaaggagct tgtaagagaa
     6421 gaaatcatta accttgttaa aaaatatgct gacaaggatg ctgcatgggt aaaagcgaat
     6481 gcaaaggatg agacaactct tgaaagtgcc cgccaaacag cagaagccgc actaagaatt
     6541 cttaccgttg attaccataa cgaagcaaga aagacaaaag gtgaagagac atggaagatc
     6601 gtcggcgaac tttatccgac atacctgaaa tattttccaa aatctgaagc atcttatgat
     6661 atgcgtttca attacgctga gtttttatat gatcataaaa aatattcaga agccggtgat
     6721 cagtatcaga cagtggctga aatgaatccc aaaggtcatc actttgaaga tgcttcttac
     6781 ggtgcagtaa gctgtttcgg ggcgttgctt gaagctgaac ataaaaaagc acagaacgaa
     6841 gctaagaacc gagtggctga agcaaaaaaa cagggtgcgg gagcaattca ggcaaatatg
     6901 gttaagaccg cagatgataa agatgccgga aagaaggatg aagttgctgc tgaattcaaa
     6961 ccaaaaccta taccagagct tcatcagaaa tacataaacg catgcgatac atatataaaa
     7021 aacataccta gatcaaaata tctggttgat atcatttatc agcaggcaat tacatattat
     7081 gctttcaacc attttgataa agcggttcct gtttttgaga tgatagttca gaaatatcca
     7141 agacatgatc ttgctgttta ttctgccgac cttatcatgg actcgttgaa tatgtctaaa
     7201 gattgggaat tgattaacaa caagtcaaga gagttcttga gaaattcagc cctgctttcg
     7261 ggaaggaaca gacttaaaac ggaccttgat aatttcaagg aaatggcgac tttctattct
     7321 gcagacattc ctcataaaaa tggaaagagt cttgaatctg ctgatagata tatggctttt
     7381 gccacagaat tcccgaaatc aaaatttaat gatgtcgcac tttataatgc ggttgtatat
     7441 tatcagaaag gcggagacct ttataaatca ataagggttc aggagcagtt cttgagagaa
     7501 agtgacgatc tttataaaaa atcgaatctt aaagacaagg taatgttcgg tctggctaag
     7561 aactatgaag cgattgctta ttatgataaa gctgcccagc tttatatcga gttcgtagaa
     7621 aaatctccga caagcaaaga cgctggggat gctgtttaca atgcggcagt tctttttgaa
     7681 agtatagggg aaacagaaaa ggcaattgac aactaccgtc tttttgttga taaatatgca
     7741 aagacggata aagaaaaatt ccagattgct cttcagtacg gttatatatg gatgagaaaa
     7801 ggaaaaacat tctatgatag agcagataag ggtttcgacc gttttcttac tgatcatctt
     7861 gatattgatg gagttaaaga ttattactac atagatgaag catgcaagaa aaaacctgtt
     7921 tctgaagtga ataagattaa agttgaaaag ggtgacccaa acacgattct tgctctttat
     7981 ggcgctaaaa agaaaatggc aagagaaatg ggtaacacaa aggaattcta taaaaatgta
     8041 gacaaagttc tccagatatt cagaggagga gagtttaaag aaaaagaaga ccttaatgaa
     8101 atatctagag acattatcgc agaagctctt cttgaagaaa ttctgccgga atttaatgaa
     8161 tatatggctt tgaagtttga taatattaaa gtcgctccca aacataaagg atatgagata
     8221 ctgcagtatg gttttgatgt tgaagacggc aaggtgatgc atcatggaga tggggatgta
     8281 agtcagctcg caccatctga tattgcaaat cttaaagcag ctgccaaaat aaaggatgat
     8341 ttcaataaag agtctcagga gaaaatgaca aagaaaattg aactcacaat ttctcttgca
     8401 gataaatatg ctcaaataat tgaaattgca aaatctcccc gtttcacacc ggcatctctt
     8461 ttttacattg gaaagattta taaagatctt actgatcaga tgtttaacgc tcctcttgca
     8521 ccgtggctta gtgaagcgca gatagctgag tataagaatt tccttgatga aaaggcgttc
     8581 ggtgcacaga aaaacgcagt tgctttcttt gaaagtgcca tgaaaaaagg atatgaaaca
     8641 tctgtttata atgattgggt ttcaaaggca aaatatgagt tgagattctt ccaggaagtg
     8701 acgggtggaa aatattatga tgagaatgaa attgttccga ctcctaatat ggttgaaact
     8761 tcttcattga taggaaagat agacatcgat tttaaatttc ccaaaattac tgatgaagaa
     8821 agaaaggaat tgaaaaagat gttaaataaa cctaaaacag caacgacgca aagtagtgct
     8881 gttgcggcac ctgctgctga aaccgtaact ccggcagaac caacaaatga gaagcaggga
     8941 gaataagtca tgaaaaagat aatactgtta ataacaataa tcgtttctct ggtttttgtt
     9001 tcatgtgctt ctgcacctga taaaaaagaa gaagtaaagg atgataaaaa ggtaatagtt
     9061 gtaacgcagg agccaaaaga acctgaagac tgggcaaaat actatactca aaaaggtatg
     9121 gaagcccttg aattgaaaga tggtaaagca gattttgata aagctgtcga atactttgag
     9181 cttgctacaa cggcatatcc ttcggcaccg atagttcatt acaatctggg tttggtttat
     9241 caaagaaaag gcgacgacca gaatgcggta aagcactaca acaaagctct tgagctggat
     9301 gcaaaatatt ccatgccgat cattaataaa gctgcaattt atgcaaacaa gttggaatac
     9361 aacagagcta ttgatgtatg tgagaagttt cttgaagaaa acgacaaaga caaaggtgtt
     9421 ttgtcagctg tagccggatt ttatcttctc aaaggtgact attccaacgc catacaatcg
     9481 gtaagaaaga tacttattca ggatcaggcc aatgtagatg ctttaaaaaa tctcggtata
     9541 ttttatttta agaaaaacga catgggtttg gcatcaatgg taacaacaaa ctcctataat
     9601 ctgaaacctg atgatcctgg tattgcaaac aacaggggcg ttttgtattc aacacagaaa
     9661 gatgattctc ttgcgatgtt atatttttct aaggcgatca gcaatgatcc aaagaacaaa
     9721 agtgccaatt ttaatgttgg ctctatagca atgaaatatg gtgatgctga aacggcatat
     9781 gaaagatttg cgatggttct ttctcaggat ccggacaatc ttcttgcaag aataggtctt
     9841 gcaatatcac taagaggtct tggccgtttt gaggattctg aaagagaata tatggcagtt
     9901 ttgcagaagg atccgaacaa tgcacttgcc acattcaatt tagcggttct tcactatgat
     9961 cacatgaaca agaaggttga ggcaaaaaga aatttcagga agtatttgtc tttggccgaa
    10021 agcgtttctg atgatcacat tgttcaccaa taccttaaag atgctgtgaa cataagacct
    10081 gtcgagaaaa cggaaggaca atagaagtaa aatttgatat ttttgcggtt atatgtatag
    10141 agtgatgcgt gttggttaat aaatgaggag gtcgccgaaa tgaagaaaat ggtgattatg
    10201 tttgttctgt tgctcgcatt cggtattgtc tgggcgcagg atcaggatac ggatgaacag
    10261 ggacgactta ttaagtaccg agaaaaaaca gttatagatt ttgaagatgt aatgcttgag
    10321 ggtcagataa aaaagccatc agggcagttt ttaattgaca ggtccaaaac aaagttcaat
    10381 tctttgatta atctgaaaaa agattttaac aaagagttgg taaggtcggt tgacctttta
    10441 aattgagtgc tatgtttgtg ttaataattt aataatgagg ccggagccat gcctgagaaa
    10501 tcaaagttga gaaaaagact actttacgat cttacgccta ttccggaggt cccggtaaac
    10561 aaaaatgtta ccggaaaact ggaaccagag atcagagttg tttatgacaa aagaatcctt
    10621 ctggatttgc agaagattaa aaagagtttt aaagttggat ctgaaaatgt tgccgactgt
    10681 gttgttgatg aaacctatgt tcctgaacag gcatatgaat ttgctactgt ttctggaact
    10741 cagtttagta tcaatctaaa ggaaaacttg aaaggagtaa tttacaaagg tgacgaaaaa
    10801 acagatctga ctgaatatgt tgctaaaaaa ggcaatacag ttgatattga tgaatcaact
    10861 cttttgatag ttgaattcgg caagataagt atctgtgcga tgaaatctca gctggagcca
    10921 tttaaatccg gtgggttttt gaagcagttt gatgctgtgc ttggaattat tatgcttcta
    10981 tctttcctga tcggtgggtt tattgttaac atgataattg aaacccctcc catagaggtt
    11041 gacatttttg aacttgacaa tagatttgca aaactcattg tgggtgaaga gcaggatgta
    11101 aaagttgagg aacttgagga agaaaagaaa gtaaaacttg aaaaaccgaa agaaggtctt
    11161 gtagaagcaa gtcagcaaag aaagtctttg ggtgaagaag gccgtgttgg cgataagaag
    11221 agcagagtcg caaacgctca gggatctgct agacgtggtc ttgatgcaaa agttgctcaa
    11281 agttcaggta ttatgggtgc gttgtcatcc ggtgccgcaa actttgaccg agtttttggt
    11341 ggtggtggtc ttggtgccgg tatggaaaaa actcttggat cagttacggg tcttgcaggt
    11401 gttgatcagt ttggttcagg tggtttggga acaagaggtt tcggtacagg tggcggtggt
    11461 aatgctctcg gcatcggtgg tttaggaaca agaggtcgtg ggggcggcgg tggtggaaaa
    11521 tatggtatgg ctgccggaca gattggcaga aaaggccgct cagatattac agcaggtgga
    11581 ggtcaggccg ttattatggg agctcttgac cgctctgtta ttgatgcata tataaagaga
    11641 aatctagcta agataagatg gtgctatgaa aaagaactta acaaagatcc gaagcttttt
    11701 ggaagaattg ttataaattt cattatttcc ggcacaggta ctgtaagtag ttcaaaagtc
    11761 aacagaacaa ctatgggtaa ccagaatgtt gaaagctgtg ttgcagatca gataaagaaa
    11821 attcgcttcc ctgcacctaa aggcggcgga atagtcattg taaattatcc gtttgtgttt
    11881 aaacactctg aagggtagga gagaaaaatg aagaaactgt tagtaattgc tgcaatactc
    11941 ttactaagct gttccatttt tgctgagtca gctgaagaga caggagatgt ttccggaaat
    12001 gaccttaatc cggtcatcag aggatttaat ttccaggttt caggcggtat actcgcatat
    12061 gttggtaaag ccggtggtga aaacgcaggc agtccacttg gtacagcttt taaattcaac
    12121 attggatatg acatcccaat aaaaaatgta gttaacattg gtgttgctgc ttctattggt
    12181 atgttacaat tcaacacaaa tcccaacgac atcggtctgg gcacaaagta taacaaatct
    12241 ccgtgggttg aggattacag tcctcttacc gcaggtcttg agattgaagt tgcatggatg
    12301 ataactcaaa gatggga
//
LOCUS       DAOOYB010000070        12282 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_5379_length_12282_cov_9.436902, whole genome shotgun sequence.
ACCESSION   DAOOYB010000070 DAOOYB010000000
VERSION     DAOOYB010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12282
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5379_length_12282_cov_9.436902"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="PL056_05675"
     CDS             <1..339
                     /locus_tag="PL056_05675"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPM46538.1"
                     /translation="PFSFKANRDIVPEESLIKKRLESVGLETDLFDKMMSEVSGGQQQ
                     RLAIVRGLLLKREIYLLDEITSNLDGTNKKLIIDIFRNMNDVSILSVGHDEEWINCAT
                     RVIKLGKGEK"
     gene            336..1142
                     /locus_tag="PL056_05680"
     CDS             336..1142
                     /locus_tag="PL056_05680"
                     /inference="COORDINATES: protein motif:HMM:NF015605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPM46539.1"
                     /translation="MNTADISVSGMIFAFAAVAVSLIILTNLKIKLRKEVVLTFARMT
                     VQLLLVGLYLQVLFDINSPFLNVAYIFIMIIAADYTILRSSGFTMALFIYTLPAYMIS
                     ITAVLVYYMIAVFSPDNVLDARYIIPIAGMLLGNSMTRTVVTLERFYTSIQREYDTYL
                     SYLFMGATYKEAVIPFFRNAYKAGLAPSLANYAAIGLVSLPGMMTGQILGGSNPMVAV
                     KYQIAIILAIFAATEISTMLALIFSLKKGILPTGFLNRSIFRKARVSIKS"
     gene            1234..2454
                     /locus_tag="PL056_05685"
     CDS             1234..2454
                     /locus_tag="PL056_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010447488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HPM46540.1"
                     /translation="MNLKKIFTKLNLGTWIMIAMFAGIGAGLILGNDAEVLRPIGELF
                     IKLIKMLVVPLVAFSIILGASSMGDAKSAGKAGITTFAYYLFTTAIAVVLGLLAGNIF
                     EPGAGLEVSTIQNMFSGEYADKGATPGFWETVSGFIPENPFLSLTNGNILQVLFFALF
                     FGFGLSFMENKKKEPVINFVSTVNDVLIWMILKVMLTAPIGVFALMADAVGTFGWDIL
                     ALVLKLLFVYTGVLLIQTFGVYPLMIRLFSKMKPLEFIKKISKAQIVALSTSSSMATL
                     PVTMEVCEKEMNVSKETSSFVLPLGATINMDGNAIYYALSALFFAQIFGIELGMAQYV
                     AIIITATLGSIGQAGVPGPTLLVVAVLLSADIPVVGLPLLFGVDRLFDMMRTAVNITG
                     DASCAVIVDSMMEK"
     gene            complement(2714..3076)
                     /locus_tag="PL056_05690"
     CDS             complement(2714..3076)
                     /locus_tag="PL056_05690"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HPM46541.1"
                     /translation="MSRVVILGASNQPGRYSNMALHSLISHGHEVVPVHPVIPVIDGI
                     KVTKKIADITGEVDTVTLYIGSSRVEEIADEIIGIKSKRIIANPGTETETLKKKALQA
                     GIEYIEGCTLVMLSSGLF"
     gene            3214..>3456
                     /locus_tag="PL056_05695"
     CDS             3214..>3456
                     /locus_tag="PL056_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane lipoprotein lipid attachment
                     site-containing protein"
                     /protein_id="HPM46542.1"
                     /translation="MKKIIFLILAAVFSAGCSFVNTSNGHIDADDDVFSFVDDDIADL
                     SDELTDPDDNPDETVTPDESVTPDESVTPDESVTPDE"
     assembly_gap    3457..3556
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3557..4439
                     /locus_tag="PL056_05700"
     CDS             <3557..4439
                     /locus_tag="PL056_05700"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HPM46543.1"
                     /translation="SVTPDESVTPDESVTPDEDIESPEKVVIATYNTHMFFDTVCDSG
                     SCASSDFEEQPSVSEYNDKVADVAEALTMINADIVLLQEVEKKICLEDLFDEMNGVYD
                     DLYIGETGYDASIDTAVITKGEITYTNKHTGSIPHPDGGTTTFTRAFLEAHINLGGRK
                     IIVFSAHFKAKSNDDPERRQAEADAALEIINDVADSNSDALIVMGGDLNDTPGSGPVS
                     TLENSSELVRVASELSSTDQATYYYDGPIAIDHIFFSVEGSGSYVSKSAEVIKDAPSW
                     YSLISSDHAALKAEFEF"
     gene            4443..5357
                     /locus_tag="PL056_05705"
     CDS             4443..5357
                     /locus_tag="PL056_05705"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPM46544.1"
                     /translation="MFRFAAVTQKPLIIPIFVPNMGCPNLCTFCNQKIVTGVSEPSLD
                     DVERTVEEYLSFSKKRKRTEISFYGGSFTAIPKETMMAYISKGAEFVDKKTVNALRCS
                     TRPDAVDEKTVKILKENHFETVELGVQTMSESLLVKMKRGHSAEDVLKGVGIIKNNGI
                     TAGLQFMTGYPDESVEDVEITIDSLKNLKPDFIRIYPFVPLEGSEMFSEISSGRKELI
                     QKEEVMERTVSLFLEAMKLKIPVIRIGLPVSSSMPDIYPHNLSQVVVGRAIERLAAEG
                     ETLFDLPSEWMTSFFTARKKFPQILVDK"
     gene            5557..6603
                     /locus_tag="PL056_05710"
     CDS             5557..6603
                     /locus_tag="PL056_05710"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HPM46545.1"
                     /translation="MKKLAVMIVTAVAWLFSCENTDYSDYDLPGDCGGKCEFVSDMNS
                     FRDAVANSAAQFSNCICLEEGLLDGEITVSKTLNIIGRKDSSSMLKRLSFYNAQNVLV
                     SDITFQNISVNDSAMFISGSSVKIENVVFSNISAGSLFGGRGVVVSGKSSDVIFENVR
                     IKKTDGTGLLIDGSHEISVISSTFSDCGFAGIWVQNSRETSGMLKVSDTVLNDNGAVS
                     LQILGKTALMVSDSQINGVKKREINMEVVGDGIVVKNGMMTQSGSVSIKNLKVTGFER
                     AGMIFDGGNGSALNGIVLENISITSENGQFGAVVQNGSEPAGFRNGIDVNTFTQNDKN
                     LTSPLYLIETIQEM"
     assembly_gap    6739..6838
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6839..8088
                     /locus_tag="PL056_05715"
     CDS             <6839..8088
                     /locus_tag="PL056_05715"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HPM46546.1"
                     /translation="VFINETVISDCVKGGINVCGGEYECGSELFFERSRIENIVPEKQ
                     TEISYGISMGPGRVTIKDSVMSGFNSFGVALWGGNEGQSIVAEIENTVISDVYGGNEI
                     FEGHAVYAEGGTVLSIAESKINDSSATFIYFSSDMAGGVLNMSDVTLENIIKTEKEQG
                     GIVLDGRMSAQMERIYIKNSRGNGIFSNGTEITANDITIDSVFSDGFGNNGSGMMLFD
                     GSDSEFNRISIKNSQTAGILMDGICFVKIENFTISQTRSDSSIFEFGVGAAVQEGAEL
                     IMESGIIDGNRECGVMAVNGKVDMNNVEIKNTMPRECYELNSCQFAPGVPFGHGISLY
                     QGSELVLGKISISSNNNGLNIENSEVVRTGGEKAIFIKNISAVNAWNISSYKDLEKSL
                     TDSEYCENQSVFTTDVQPVREGI"
     gene            complement(8091..9377)
                     /locus_tag="PL056_05720"
     CDS             complement(8091..9377)
                     /locus_tag="PL056_05720"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM46547.1"
                     /translation="MNGAAKDKNLKLLKELHEYSFELAEKDHTEMFDFIVTKARELFS
                     VKACWISTFNNETSEILVKTIALPGNEISIINNLLKKNVVGTTIKLTPDQYQKMITEK
                     ISPPLSLKEVVLGSIPETVCEVVQKTLGFGWFIGISLVNHGHLFGNIFFAGHRDQTPI
                     EEEELLIFRAITSTAMAKKETEMKLLKAKEAAESANKAKSDFLSNMTHELRTPLCGII
                     GFTDLLETETKLDETQTEFVQMLKSSAKTLLDLVNDTLDLSKIEAGKLELHTQETDLE
                     ALFHEVFKTFEPLAKEKGLKASLMLPENIPQKIFVDQLRLKQILLNLTGNAVKFTNSG
                     FVNLKLEYFKEKKNLPFLQVSVEDSGIGIEQQKLHKVTENFYQADTSITRKFGGTGLG
                     LAITDKLLRKMNSSLEISSTPQKGSTFSFKIPLSDK"
     gene            complement(9374..>10294)
                     /locus_tag="PL056_05725"
     CDS             complement(9374..>10294)
                     /locus_tag="PL056_05725"
                     /inference="COORDINATES: protein motif:HMM:NF012246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RuBisCO large subunit C-terminal-like
                     domain-containing protein"
                     /protein_id="HPM46548.1"
                     /translation="AVEICGHELTQFLTVLFGNISLLKGIRLLKINQHNYLKNISTGP
                     RFGIEGLRKLTGVHDRALLCGAIKPMGLSPSTLAEMAADFTLGRIDLIKDDHGLADQP
                     FSRFRERVKAVSDTVNEINSKTGSKTLYAPSISASASEVIDRAHFAKEAGAGALLIAP
                     FVTGIDFARMISEDDSIALPLIAHPALSGSFVNSDESGISHYALFGQIMRMSGCDASV
                     FPSYGGRFSFSKDECSSIQKGCTEPFAGYSPIFPAPGGGMTLERMSELKKFYGNDAMF
                     LIGGGMFQNNGNISENVKRFKAAYLKMELS"
     assembly_gap    10295..10394
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10395..11257)
                     /locus_tag="PL056_05730"
     CDS             complement(<10395..11257)
                     /locus_tag="PL056_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HPM46549.1"
                     /translation="MEIFSKVHGHNLVKWAADKKEVIVLSADLTSSCEADLFRDAYPD
                     RFFSLGIAEQNMLSWAAGMAGEGFIPYIHTFAVFIYRRAYDQVAMSAAYSNIPVKMIG
                     FLPGVTTPGGASHQAIEDISLMRSLPNMTVLETGDATDVETVLDTAHKINGPVYIRML
                     RGEIPRLFKDPLIFNRSRVISNGTDITLLTSGICTEEAMRATKLMKEKGVSVCHHHIS
                     TLKPFTDPQVENAISQAKFGVITMENHSVIGGLGTCVAEIIASKGLNKKLVKLGIQDR
                     FLHGASRRYLM"
     gene            complement(11259..12161)
                     /locus_tag="PL056_05735"
     CDS             complement(11259..12161)
                     /locus_tag="PL056_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012003712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HPM46550.1"
                     /translation="MNIQRIQHVADNIRKRVLTHVISQNGGYMSQACSSAETFSALYL
                     HIMSMPELDTPLKPGLFQGVPSADNMSYKTGAEYNGGHVEGFDRFYLSPAHYALVVYA
                     LLIETGRMTADGLDYFNRDGYSVEMIGAEHSPGMEVTAGSLGQCISRAAGSALARKMK
                     NDNGNNFVYLSDGEFQIGQTWEAIQAASHYKLDNLIIFADMNGQQCDGPVGNVMEMGS
                     VADRLRSFGAEVVTVDGHDIEALCKSVETPHENKVLAVLCKTDPCRGLEILRRNAPKL
                     HYLRFKSDSEKAEYTQILNELGGK"
BASE COUNT         3494 a         2337 c         2609 g         3542 t
ORIGIN      
        1 cctttttctt tcaaggcaaa ccgtgacatt gttcctgaag aatcgttgat aaaaaaaaga
       61 ttggaaagtg tcgggcttga aactgatctg tttgataaaa tgatgagcga agtttcaggc
      121 ggtcagcagc agcggcttgc aattgtgaga ggccttcttc ttaaaagaga gatctatctt
      181 cttgatgaga taaccagcaa tctggatgga acaaacaaaa aactcataat tgatattttc
      241 agaaatatga acgatgtcag tatattatct gtcggccacg atgaagagtg gataaattgt
      301 gcaaccagag tgattaagct tggcaaaggt gaaaaatgaa tacggctgat atttcagttt
      361 caggaatgat atttgcgttt gccgctgttg cagtatcgct gataattctt acaaatctca
      421 aaatcaaatt gagaaaagag gttgtgctca cttttgctag gatgactgtt cagcttctcc
      481 ttgtcgggct ttatcttcag gttctttttg atattaacag cccattttta aatgttgcct
      541 atattttcat tatgataatt gcggcggatt acacgatatt aagaagcagc ggttttacga
      601 tggcgctatt tatttataca cttcctgcct atatgatctc gataacagca gtgctcgttt
      661 attatatgat tgcggtcttt tcgcctgaca atgttcttga tgcgagatat atcattccga
      721 tagccggaat gcttcttgga aacagcatga ccagaactgt tgtgacgctc gaaaggtttt
      781 atacttcgat ccagagagag tatgacacat atctgagcta tcttttcatg ggggcgactt
      841 ataaggaagc ggtcattccg tttttcagga atgcatataa agccggactt gcaccgtcac
      901 tggcaaatta tgccgccatc ggacttgtct cgcttcccgg aatgatgacc ggacagatac
      961 tcggcggaag caaccccatg gtcgccgtga aatatcagat cgcaataata cttgccatat
     1021 ttgccgccac cgaaatatca acaatgctcg ccctcatttt ctcattaaaa aaagggatac
     1081 tcccaaccgg ctttctcaat aggtcgatct ttcggaaagc aagagtttct ataaaaagct
     1141 gacatgaacc ttaaccttga aataataaat tttcacaata caatcaaagg gatagttgat
     1201 aaggaattct aaaacagatc cggagatgat gctatgaatt taaagaagat cttcactaaa
     1261 cttaatctag gcacatggat aatgatcgca atgtttgcag gcatcggggc tgggttaatt
     1321 ctcggtaatg atgccgaagt actgcgtccg ataggagagc ttttcattaa gctgataaaa
     1381 atgcttgtcg ttccgcttgt cgctttttca ataattctcg gtgcttcaag catgggagat
     1441 gcaaaaagtg cgggaaaagc tggaattaca acctttgcat attatctttt tacaacggcg
     1501 atcgctgtag ttcttggact tctggcagga aacatttttg aaccgggagc cggtcttgaa
     1561 gtttcaacaa ttcagaacat gttcagcgga gaatatgctg ataaaggggc gacccccggc
     1621 ttctgggaaa cggtttcggg ctttattccg gaaaacccgt ttttatctct tacaaacgga
     1681 aacattctgc aagtgctctt ttttgctctg tttttcggat tcgggctgag ctttatggag
     1741 aataagaaaa aggagccggt cataaatttt gtttcaacgg tgaatgatgt tcttatctgg
     1801 atgatactca aagtgatgct gaccgctccg attggtgttt ttgcattgat ggctgatgca
     1861 gtcggcacat tcggatggga cattctggca cttgttctga agctcctttt tgtatatacc
     1921 ggagttcttc tgatacagac tttcggagtc tatccgttga tgatcagact tttctcaaag
     1981 atgaaaccac ttgaatttat taagaaaata tcaaaagccc agattgttgc actttcaaca
     2041 tcaagctcta tggcaactct tcctgtcaca atggaagtct gcgaaaaaga gatgaatgtt
     2101 tcaaaagaga cctcttcgtt tgtcctgcct ctgggcgcca cgatcaatat ggacggaaat
     2161 gcgatatatt atgcgctgtc tgcacttttt ttcgctcaga tattcggcat tgaactcgga
     2221 atggctcaat atgttgcgat cataatcacc gctaccctcg gctcgatcgg tcaggcaggg
     2281 gttcccggac ctacacttct tgttgtcgca gtccttcttt cggcagacat tccggttgtg
     2341 ggactgccgc tccttttcgg agttgaccgg ctctttgaca tgatgagaac cgcagtgaac
     2401 attacagggg atgcaagttg tgctgttatt gttgacagta tgatggaaaa ataaaaacat
     2461 tcgtgtataa ctgaaaccgt cttttttaat gaattgattt taatctgcaa ttatattatt
     2521 attatcgtat gaacttggag aaaattatat gagaaatttg ttgcctgtac tgatttttat
     2581 gacagcgttg ttttgtgcat taatttgttc cggaaacagt gattatggga agttaggcga
     2641 agaatgaaat ttcttttctg aaatatcttt taccgttcgt taagaaaaat cggtcatcga
     2701 tggcaataat gtttcagaac aatccgcttg aaagcatgac gagtgtgcag ccctcgatat
     2761 attcgatgcc ggcttgaaga gcttttttct ttaaagtttc agtttctgtt cccggatttg
     2821 caataatcct tttcgacttg atcccgatta tttcatctgc aatttcttca acccttgaag
     2881 aaccgatata aagtgtgaca gtatcaactt cacctgttat gtcagcgatc ttctttgtaa
     2941 cctttattcc gtcgatcacc ggaataacag gatgaaccgg aacaacttca tgaccgtgac
     3001 tgataagcga atgaagcgcc atgttcgaat atctgcccgg ctgattgctt gccccgagaa
     3061 taacaactct gctcatattt ccctccagaa aaataaaaaa cccagcacat tgaaaaatta
     3121 caccttagaa aaaaaattgc aataaaaggg caacatttga catcctctca tcagataata
     3181 aaatgtagtc cggttatttt taaggagata gttttgaaaa aaatcatatt tctcattctt
     3241 gcggcggtat tttctgccgg atgcagtttt gtcaatacga gcaatgggca cattgatgcc
     3301 gatgatgatg tcttttcttt tgttgacgat gacattgccg atctcagtga cgaactcaca
     3361 gatcccgatg ataaccctga tgaaacagtg actccagatg aatcggtgac tccagatgaa
     3421 tcggtgactc cagatgaatc ggtgactcca gatgaannnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnatcg gtgactccag atgaatcggt gactccagat gaatcggtga
     3601 ctccagatga agatattgaa tctcctgaaa aagtggtgat tgcgacatac aacacccata
     3661 tgttctttga cacagtatgc gattccggct catgtgcttc ttcagatttt gaagaacagc
     3721 cctccgtttc cgaatataat gataaagttg ccgatgtggc tgaagctctc accatgataa
     3781 atgccgacat tgttcttctg caggaagtcg aaaagaagat ctgccttgaa gacctttttg
     3841 atgaaatgaa cggagtgtat gatgatcttt acatcggcga aacgggatat gatgcatcaa
     3901 ttgatactgc agtcataaca aaaggggaga taacctacac aaataaacat accggctcga
     3961 tccctcatcc agacggaggg acaacaactt tcacaagggc gttccttgaa gctcatatca
     4021 acctcggagg gagaaaaata atagtctttt ccgcccattt caaggcaaag agcaacgatg
     4081 atccggagag gagacaggcc gaagcggatg cggcacttga aataataaat gatgttgcag
     4141 acagtaactc tgatgcattg atagtgatgg ggggagatct caacgacaca cccggttcag
     4201 ggccggtatc aactcttgag aattcatcag agcttgtcag ggtcgcatca gaactttctt
     4261 caacagatca ggcaacttac tattatgacg gtcccattgc cattgatcac attttcttct
     4321 cagttgaagg gtcaggatcg tatgtttcaa aatcagcaga agtaataaaa gatgctccga
     4381 gctggtattc gctgataagt tcagatcatg ccgcgttaaa agcggaattt gagttctaaa
     4441 aaatgttcag atttgccgct gtaacacaaa aaccgctgat aattccgata tttgtcccta
     4501 atatgggttg tccaaatctg tgcacattct gcaatcagaa aattgtgaca ggggtttcag
     4561 agccctctct ggatgatgta gaaaggaccg ttgaagaata tctttcattt tcaaaaaaaa
     4621 gaaaacggac ggaaatatca ttctacggcg gaagttttac agccattccc aaagaaacga
     4681 tgatggctta catatcaaaa ggagctgaat ttgttgataa aaagacagtt aatgcgttaa
     4741 ggtgctcgac aagaccggat gccgttgacg aaaaaacagt gaagatatta aaagaaaatc
     4801 attttgaaac agttgagctc ggtgtccaga caatgtcgga atctctgttg gtaaaaatga
     4861 aaagagggca ttcagcggaa gatgttctca aaggtgtcgg aatcataaaa aacaatggaa
     4921 taacagcagg tctccagttt atgaccggat atcctgatga aagtgtagaa gatgttgaaa
     4981 taactattga ttcactcaaa aatctcaagc cggactttat aaggatatat ccatttgtcc
     5041 cccttgaagg gagtgaaatg ttttctgaga tttcatcagg aagaaaagaa cttattcaga
     5101 aagaagaggt tatggaaaga acagtttctc tctttcttga agcaatgaag ctgaagattc
     5161 ctgtcatccg gatcggtctg cccgtttcat cttcaatgcc cgatatttat ccgcacaatc
     5221 tctcccaagt cgttgtcgga agggctattg aacggcttgc ggctgaaggc gagaccttat
     5281 ttgatctgcc ttccgagtgg atgacatcat tttttacggc aagaaaaaaa tttcctcaaa
     5341 ttcttgttga caaatgaaaa aatcgtaata aattaaattc cgttagtgct attcagtaca
     5401 ttcttactca atgttctatg gttattttct tttacgattg aaaatccccg ctcaggggat
     5461 tctcatcttt taaaaaagtt ctaatataaa attgataaat agcttttccg gagtaatata
     5521 aataagcttg tggtttaaga atatggagcg gatgaaatga aaaagcttgc agtcatgata
     5581 gttacagcgg tcgcctggct tttctcatgt gaaaatacgg attacagcga ttacgacctt
     5641 cccggggatt gtggcggaaa atgtgaattt gtaagtgaca tgaactcatt ccgtgatgcc
     5701 gttgcaaatt ccgcggcaca gttctcaaac tgcatctgtc ttgaagaagg gttgctggat
     5761 ggtgagataa ctgtttcaaa aacattgaac ataatcggaa gaaaagacag ttcatccatg
     5821 ttgaaacgcc tctcttttta caatgctcaa aatgttcttg tatcggacat aacttttcag
     5881 aatatttcag ttaatgactc tgcaatgttc atttcaggaa gctcggttaa gattgaaaat
     5941 gtcgtttttt ccaatatcag tgccggctct ctttttggcg gaaggggagt tgttgtttct
     6001 ggaaaaagtt cggatgtcat ttttgaaaat gtaaggatca aaaaaactga cggaacaggt
     6061 cttttgatag acggatcaca tgaaatttca gttatatcaa gcactttttc agattgcggt
     6121 tttgcaggaa tatgggtcca gaatagtcgg gaaacatcag ggatgcttaa ggtctcagac
     6181 accgttctta atgataacgg tgcagtttca ttgcagatat tgggaaaaac agctctaatg
     6241 gtttctgatt ctcaaataaa cggagtgaaa aaaagagaga taaatatgga agtggtaggg
     6301 gatggaatag ttgtcaagaa cggaatgatg acgcaaagcg gatcagtatc aataaaaaac
     6361 ctcaaagtta caggatttga aagagccgga atgatatttg acggtggaaa cggctctgcg
     6421 ctaaacggaa tagtccttga aaatatcagc atcacttctg aaaacgggca gttcggagca
     6481 gttgtgcaga atgggtcaga gccggcagga ttcaggaacg gaattgatgt aaacacattt
     6541 acgcaaaatg ataaaaattt gaccagtccg ctatatctta ttgaaacaat tcaggaaatg
     6601 taacatgctg aggattgttt ttctcctttt attttttatt tcctgcagtg tggaaaaacc
     6661 tgttcctgaa agtgatcatg aaaatagaga tgactcagaa cttcaatgcg gaaattatga
     6721 agtgactgtc gaaaccccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnct
     6841 gtatttatta atgaaaccgt tatatcagat tgcgtgaaag gggggataaa tgtctgcggc
     6901 ggggaatatg agtgcggaag tgaactgttt tttgaaagaa gcagaattga aaatatagtt
     6961 cctgaaaaac aaactgagat aagttacgga atttcaatgg gtccgggcag agtaacaatt
     7021 aaagattctg ttatgtcggg atttaattca ttcggggtcg cactttgggg ggggaatgaa
     7081 ggtcagagca ttgtcgcaga aatagaaaac actgtaatat cagatgttta tggagggaat
     7141 gaaatctttg aaggtcatgc tgtatatgca gaaggaggga cagttctttc aattgcggaa
     7201 agtaaaataa atgacagttc cgcaacattt atctattttt catcagatat ggcgggtgga
     7261 gttctgaata tgtccgatgt tacattggaa aatatcatta aaacagagaa agagcagggg
     7321 ggaatagttc ttgacggcag aatgtctgct caaatggaga ggatttatat aaaaaattca
     7381 aggggtaacg ggatattttc aaatggaact gaaattactg caaatgatat aaccatcgat
     7441 tctgtttttt ctgacggttt tggaaacaac ggctcaggaa tgatgctttt tgacggaagc
     7501 gattcagaat tcaaccgtat ttctataaaa aacagtcaaa ctgcgggaat tctcatggat
     7561 ggcatctgct ttgtaaaaat agagaacttc acaatttcac aaactagatc agacagcagt
     7621 atttttgaat tcggagtggg ggccgctgtt caggaaggcg ctgaattaat tatggaaagt
     7681 gggataattg atgggaacag agagtgcgga gttatggctg tgaacggaaa agtcgatatg
     7741 aacaatgttg aaataaagaa cacaatgccg cgggaatgtt atgaactgaa cagctgtcaa
     7801 tttgcccccg gagttccttt cggtcacggg atttcgcttt atcagggttc tgaactggtt
     7861 ttaggcaaga tctctatttc cagtaacaac aacggactca acattgaaaa ctctgaagtg
     7921 gttagaacgg ggggagaaaa agcaattttc ataaaaaaca taagtgccgt caatgcatgg
     7981 aacataagca gttacaaaga tcttgagaaa agtttaactg attcagaata ttgtgaaaac
     8041 cagtcagttt ttacaactga tgttcagcca gtcagggaag ggatatgaga ctacttgtca
     8101 gacaaaggta ttttaaagga aaaagtactc cctttctgag gtgtgctcga tatttcaagg
     8161 gatgaattca tttttcttaa aagtttatct gtgatagcga gtccgagtcc tgttccgccg
     8221 aattttcttg tgatggaagt gtccgcctga taaaaattct ctgttacttt atgaagtttc
     8281 tgctgttcta tccctatgcc tgaatcttca actgaaacct gaagaaaagg aagattcttt
     8341 ttttccttaa aatattcaag tttaaggttc acaaatccgc tgtttgtgaa tttcacagca
     8401 ttgcctgtca agttcaaaag gatctgcttt aacctcagtt gatcaacgaa tattttctga
     8461 ggaatatttt caggaagcat gagagatgct ttaagacctt tttcttttgc aaggggctcg
     8521 aaggttttaa aaacttcatg gaaaagagct tccaaatcgg tttcctgtgt gtgcagttca
     8581 agtttgcctg cttcaatttt gctcaggtca agagtgtcat ttacgagatc gagcagtgtt
     8641 tttgccgaac ttttaagcat ctggacaaac tctgtctgtg tttcatcaag ctttgtctcg
     8701 gtttcaagaa ggtcggtaaa tccgattatt ccgcaaagag gagttctgag ttcgtgggtc
     8761 atgttcgaaa gaaagtccga ttttgcctta ttcgctgatt cagccgcttc ttttgctttg
     8821 aggagtttca tttcggtctc ttttttagcc attgctgtcg aagttatcgc tctgaaaata
     8881 aggagttctt cttcttcgat cggggtctga tcacggtgtc ctgcaaaaaa tatgttgccg
     8941 aaaagatgac cgtgattgac cagagatatt ccaatgaacc atccgaaccc gagcgttttc
     9001 tgaacaactt cacacacagt ttcaggaatg gagccgagaa ccacttcctt taaagataga
     9061 ggcgggctga tcttttcagt tatcattttt tgatattgat caggtgtcag ttttatggtc
     9121 gtgcccacaa cattcttctt taagagattg ttgatgatcg aaatttcatt ccctggaaga
     9181 gcgatggttt taacaaggat ttcagaggtt tcattgttga aagttgatat ccagcatgct
     9241 tttactgaaa agagttcccg tgcttttgta acgatgaaat caaacatttc agtatgatcc
     9301 ttttcagcaa gttcaaatga gtattcatgc agttctttaa gtagtttcag attcttatct
     9361 tttgcagcac cattcatgac aactccattt tcaaataagc agctttgaat cttttcacat
     9421 tttctgaaat atttccatta ttttgaaaca tccctccgcc gatcaggaac attgcatcat
     9481 ttccgtaaaa ttttttgagt tcagacattc tttcaagtgt cattcctcca cccggagcag
     9541 gaaatatcgg actgtatcct gcaaatggct ccgtgcatcc tttctgaatt gaactgcact
     9601 catctttaga aaacgagaat cttccgccgt aacttggaaa aacacttgca tcacaccccg
     9661 acattcgcat aatctgacca aaaagggcat agtgacttat tccgctttca tcagaattca
     9721 caaaacttcc tgaaagtgca ggatgggcaa tgagcggaag agctatggaa tcatcttcag
     9781 aaatcattct tgcaaagtcg atccctgtca caaacggggc gataagcaaa gcgcctgccc
     9841 cggcctcttt tgcaaaatgt gcacggtcaa taacttctga agcggatgca gaaatgcttg
     9901 gagcataaag agtttttgag ccggtttttg aatttatctc atttactgtg tcactcactg
     9961 ctttaaccct ttccctgaaa cggctaaacg gctgatcggc aagtccgtga tcgtctttga
    10021 tgaggtcaat tctgcccaga gtaaaatcgg ccgccatttc tgcaagtgtt gaggggctaa
    10081 gtcccatcgg ttttatcgct ccgcaaagaa gcgctctgtc gtgaacaccg gtcagttttc
    10141 ttaatccttc aattccaaat ctcggaccgg ttgatatgtt ttttaagtaa ttatgctggt
    10201 ttatctttaa cagccggatc cctttaagca aagatatatt gccgaaaagg acagtgagaa
    10261 actgtgtaag ctcatgtccg cagatctcaa cggcnnnnnn nnnnnnnnnn nnnnnnnnnn
    10321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10381 nnnnnnnnnn nnnnttcatc agataccgtc ttgaagcacc gtggagaaat ctgtcctgaa
    10441 ttccgagctt tacaagtttt ttattcagtc ctttggaggc aataatttca gcgacacagg
    10501 ttccaagacc gccgataact gaatgatttt ccatggttat cacaccgaat tttgcctgac
    10561 ttatcgcgtt ttcaacttga gggtcagtga acggtttgag agttgaaatg tggtggtggc
    10621 agacagaaac acctttttcc ttcatgagct ttgtcgccct cattgcttct tcggtgcaga
    10681 ttccgcttgt aagaagagtg atatcagttc cgtttgaaat gactcttgat cggttgaaaa
    10741 tgagcggatc tttgaaaagc cgcggtattt cgcctcggag catcctgata tagaccggtc
    10801 cgtttatttt gtgtgccgta tcaagaactg tttcaacatc ggttgcatcg cctgtttcaa
    10861 gaactgtcat attgggaaga gaccgcatca gcgaaatgtc ttcgatcgcc tgatgacttg
    10921 ctccgcccgg agttgtaacg cccggaagaa agccgatcat tttgacagga atattgctgt
    10981 atgccgcact catcgcaacc tgatcataag cccttctgta aatgaaaacg gcaaaagtgt
    11041 gaatataagg aatgaatcct tcaccggcca ttccggcagc ccagctcagc atgttctgtt
    11101 cagcaattcc aagagagaaa aaacggtcgg ggtatgcatc tctgaaaaga tcggcttcgc
    11161 atgaacttgt gaggtctgcg ctcagaacga tgacctcttt tttatctgcc gcccatttga
    11221 caagattgtg tccgtgaact ttagagaata tttccatgtc actttcctcc aagttcgtta
    11281 agtatttgag tatattcagc tttttcagag tcagacttaa atctgagata gtggagtttc
    11341 ggagcgtttc ttcttaaaat ttcaagtcct ctgcatggat ctgttttgca aagaacggca
    11401 agaactttgt tttcatgagg agtttcaacg cttttgcaaa gtgcttcaat gtcgtgtccg
    11461 tcaactgtca caacttctgc tccgaaagag cggagacggt ctgcgactga tcccatttcc
    11521 atgacatttc cgaccggtcc gtcacactgc tgaccgttca tgtcggcaaa aatgatcaga
    11581 ttatcaagct tataatgact tgccgcctgt atcgcctccc atgtctgtcc tatctggaac
    11641 tctccgtccg aaagatatac aaaattattt ccgttatcat ttttcatttt tcttgcaagg
    11701 gcggaacctg ctgcccgact tatgcactgg ccaagtgagc cggcggtcac ctccatcccc
    11761 ggagagtgtt cggctccgat catttcgact gaatatccgt cacggttgaa ataatcaagc
    11821 ccgtcagcag tcatccttcc cgtttcgatc agaagtgcat aaacaacaag ggcatagtgt
    11881 gccggagaaa gataaaaccg gtcaaaacct tcaacatgtc cgccgttata ttcagcaccg
    11941 gttttgtaac tcatgttatc agcagacgga accccctgaa aaagtcccgg tttcagcgga
    12001 gtgtcaagct ccggcatgct cataatatgt aaataaagag cagaaaaagt ctctgcactt
    12061 gaacatgcct gagacatata tccgccgttc tgagagatga catgcgtaag cacccgtttt
    12121 ctgatattgt ctgcaacatg ctgaattctt tgaatattca tagtattttc tccacaatcc
    12181 tgaaataacc cggctgatta tatctgattg gttactctaa acaagatatt tctaaaatct
    12241 tatcaccccc aaaaaaaata aaataaattt cactcaaatc ta
//
LOCUS       DAOOYB010000071        12187 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_5442_length_12187_cov_10.944197, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000071 DAOOYB010000000
VERSION     DAOOYB010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12187
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5442_length_12187_cov_10.944197"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..718)
                     /locus_tag="PL056_05740"
     CDS             complement(68..718)
                     /locus_tag="PL056_05740"
                     /inference="COORDINATES: protein motif:HMM:NF018595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HPM46551.1"
                     /translation="MEKKCDGPKKPRITCTVHSPLMVSDLKNLFDCNGNALSTKPVMS
                     LCRCGKSEKMPYCDGTHTKEGLDLKKQSDRIPDRNKDYTASDIIVHFNLGICSHDGSC
                     LKLKPVFDRHRRPWILPELGNKDAIIETIKKCPSGALSYTVDGVCYKDLFDRDPAIKV
                     AKGGPLMVEGYIEFKDDQNSCPESKEHYCLCRCGQSKNHPFCDGAHLSCGFYEEGK"
     gene            complement(788..1156)
                     /locus_tag="PL056_05745"
     CDS             complement(788..1156)
                     /locus_tag="PL056_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46552.1"
                     /translation="MIVIPKETLVIGIDKWFVENCVKGDFFWFGHPFENSKAKGFISP
                     QKEFSIKLPVTLIVDLSEATEADILSFDLENTIFYSEEWKGDASKVASFIKNIINFKD
                     GVPDIVKKDHQLMQGDLKSC"
     gene            complement(1163..1651)
                     /locus_tag="PL056_05750"
     CDS             complement(1163..1651)
                     /locus_tag="PL056_05750"
                     /inference="COORDINATES: protein motif:HMM:NF014602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HPM46553.1"
                     /translation="MKCFKPGDLAVYPNYGVGKVVSIEHRPMGESEVKCYVVSMVSSG
                     SKVFVPVDSADKMGLRTITKMECTEDVYSCFKHRNLDLLKMNWNKRYRFLQDKMKTGK
                     ITDVASVISDLMFLKKKKGLSYGEDKLLNEAEEILSTELSISENLPKDDVMSKIHSSF
                     KK"
     gene            complement(1750..2682)
                     /gene="miaA"
                     /locus_tag="PL056_05755"
     CDS             complement(1750..2682)
                     /gene="miaA"
                     /locus_tag="PL056_05755"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HPM46554.1"
                     /translation="MVIFILGPTASGKTRVSVELAKEINGEIIGADSVQIYRDLVIGA
                     ASPTLEEMEGVPHHLIGTHDLKDEISAGIYADLALDAINDINSRGKVPIIVGGTNFYV
                     DALINGLSPVPELDDVARKDYNDSLKLFSTEELYTKLLETDPLWAERISSKNDRQRIR
                     RGLEVFELTGKKLSEWNSLERVKKYPGEFFSFAISSEREELYEKINIRSKAIVKSGLV
                     DEVRKVNSMGFNVLNCRPLNSIGYAQADLFLKGLISTEDELIKAIAQNTRHLAKRQMT
                     WLKKMDYVIWKDVYCIIKSVLDALQNRYQDVSKK"
     gene            complement(2682..4370)
                     /gene="mutL"
                     /locus_tag="PL056_05760"
     CDS             complement(2682..4370)
                     /gene="mutL"
                     /locus_tag="PL056_05760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HPM46555.1"
                     /translation="MGRIKLLTEKTINQIAAGEVVERPASIIKELVENSIDAGALNIF
                     IDIENGGKKRITVKDDGCGMTEDDVFMALERHATSKINDIADLEKISTMGFRGEAIPS
                     IAAVTHFSISSAISHGEGFRIVSKNGVITENGPVSMPKGTEISCSNLFHNIPARKKFL
                     KSDEREMAHIKEMIQKFSIINYKTGFSFAHNGRNLFTLNPSPSVSVRISDIWKLNREM
                     IRSFHMERDGISLTVHVPSPFEAPVSLTAVSVNGRIVNDRVINGAILRTFREQVGGEF
                     RSPVVILLEVQNGTVDINVHPSKLEVRFRDPFIISELISNSITGALKSFRPVEKIETQ
                     AGQAFEGVTMTVSEKESPESYSGEIFSPENENLFEMKIRDYKKLGVLFGVYQIIEMTD
                     RVIFLDQHASHERITFTALKKASELKSGMSQMLVAPIIVGLSDIEKDIISSNIILFSN
                     AGFLIEQFDESSVAVRAVPAIGFDADWKGVVKEMAGELENYGSSGVMEDIFMSRLATA
                     ACRSSVKRNDLLTSLEIDSLIEDINHSESLTCPHGRPFFFSMSRNEFEKKVKRQ"
     gene            complement(4374..5657)
                     /locus_tag="PL056_05765"
     CDS             complement(4374..5657)
                     /locus_tag="PL056_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46556.1"
                     /translation="MKEGKIDFTSVLFVFILLSVGVFIFLFVNAQQDRYDYFIYLYGA
                     EVAPGSKALLKVISRDGEFVTNPEIFINGRKSATSLIDIRPDLREIAVVVGKSEVKFP
                     VRYSDLENIRVPAPVRTHISAKELENVPFPAVSGTKTVFLLPDQFRAVPEFETKVHFY
                     CLDGDGPCREREIIINGIAKELKEGYLSFTTIFTHEKNVNISFSDGSSVIAKVPYNGK
                     MFKFYTDDSGMMLASLSDTSNVHVDCYSKERWIGTDIVAINNAGTRLPDGYMNCETVQ
                     ASFNSSSPGATYVVFSRSPDLKGEVDDPYYSGLVQFLQKFPLQAQESFIRSYNSSFFM
                     PLPLIFSGEVLEKEFNEKKSGELNFYWWMILISSLAGLMLFIRTVFRKMKVVEGIDGE
                     LISRSMNNQRLILAGAIVLYAGVIFLLLYLLKNLA"
     gene            complement(5666..6544)
                     /locus_tag="PL056_05770"
     CDS             complement(5666..6544)
                     /locus_tag="PL056_05770"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HPM46557.1"
                     /translation="MRDFKVCTAIRNKDFEDSMAEIIRNEGWNHSNVNVDDIVNEHRR
                     GALFFIVEDNYTSILDLIELHNSCRLNFLPTVILSSAGSKHANWADHVRYTDKYFRAP
                     MDGFKPVAVQVIKTVLAFTNAHKYKNFFKFREFVDNSFKGCSHFSVFMNDVLTRMLEM
                     LYAGRGSMMLLNKSGNLVIEASTKKNLIGLEVEPKPGSVAWTVIDTGKPVFVENIDND
                     SRFKKTEGYSKDYFMSLPVFINGKIEGVLNLSDKMVSLLFDSSDQKNADRLLKIIEPY
                     LFIEKLKRSALSDILK"
     gene            6688..7965
                     /locus_tag="PL056_05775"
     CDS             6688..7965
                     /locus_tag="PL056_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006194097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HPM46558.1"
                     /translation="MKEKPSPINTIWLMMVLISVVAASYTGKMSGLTASVFESAKSAV
                     TLAIGLVGAMALWLGIMKVLEEAGALKLIAKAVRPVMTRLFPDVPHDHPAMSAMVMNI
                     AANMLGLGNAATPMGIKAMTELDKLNREKGTATNAMCLFLAINTSSVTLLPLGVITVR
                     ASAGASEPASILIPSILATILSTSSAIIASKLLQSKNSDAAISEEKAEEEKTPANYEK
                     PEIFKIVAVSLFIALVAGGIYFSASANTEPVSWRSFMQQLSDWLIPVIMVAFLLFGYL
                     KGVKLYETVTEGAKEGFNVAVKIIPFMVAIFVAVGMFRSSGALDIMVNIFNPLTSLIG
                     MPAQALPMAFIRPLSGSGAFGIMSEIVSKEPDSFLSYLVSTMQGSTETTFYVLAVYFG
                     AVGIKNARHAVPAALIADGTGIIGSVIICRILF"
     gene            complement(7972..9609)
                     /locus_tag="PL056_05780"
     CDS             complement(7972..9609)
                     /locus_tag="PL056_05780"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HPM46559.1"
                     /translation="MSSNVEFLALGGLKEVGKNCLLIKYEDQAIMVDCGMGFPGNDDF
                     MEDDFFIPDFDIINELDIELLGCVITHGHEDHIGGVPYLLQKFNVPVYMTKFPKMILE
                     ERIGRYAKYKNRIESFSSKKTPEINLGPFRVEMFDVPHSIPEAKGLIIDVGGITIVHT
                     GDFKYDGKKGSPFKGRVPKKVDVLLSDSTNIENTGHSESESSIIPNITQIIENAPGRV
                     IATGFSSNTTRIKNIIDISLSKGRTVGLLGRSVCSYTRIASELGHLKLPASLLTEQTK
                     INRVPENKLTLIVTGSQAEPRSVMKRMSLDMMKSVSVKFGDTIFFTSKNIPGNELSIG
                     RMIDNLVEKGADVYYENTDDIHVSGHAKQFDIVQCFKDVNPDLIIPVHGHRRFLELSA
                     KLAERNGFNAQVITDGEMLSLRKNKRPVISHKYDLTVKVVSNGDPDLVDIENIRERKK
                     IAKAGIMTVMMTVDFLANSLLSKPRIVSSGIASVPKMKKIEREVKDIIDNYFKDELAD
                     EPFWKDVEEEIRIRVRRHLFAITRKKPIVNAIIVNLD"
     gene            complement(9636..10886)
                     /locus_tag="PL056_05785"
     CDS             complement(9636..10886)
                     /locus_tag="PL056_05785"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPM46560.1"
                     /translation="MNVKWFLALTISVAVIVSAIVYDLHFRKDVNLHVSWSSAGSKRK
                     IETTYVQVPEGKYDDMFEEMIGTENWKTAKQLYRKNAGLNRFMAGDIFSVSTLGEKVL
                     SYNLHHYKNRIIGEKISFIRKGRNFEKETYEISLEVKTVVKYFKIKESLAKDDPQFYE
                     MARERLVWDWGILDKLMPGDSVSFMVKGIFDHDILVHAFGILGLSVRSSTIGDFTMTA
                     FRDATYGDYFVASHGMMLSPPGQFRSPIDSGRISSFFGYRSDPFNKRKKFHNGVDIIA
                     KIDTPVRAADAGTVSFVGKKGGLGNTIVLEHGNGMKTVYGHLNRFLVLSGYTVQKGEV
                     IAGAGNSGRSTAPHLHFTVLLDGKSVDPMQFTYERVWSAPFDIAGGFRNVSASRTAQL
                     EEVMDKARTVFVQEKYADIMSTMN"
     gene            11026..11832
                     /locus_tag="PL056_05790"
     CDS             11026..11832
                     /locus_tag="PL056_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HPM46561.1"
                     /translation="MTKSPKEAGTEKRILEKLKGKPLILAKEIFSDPEIIHLQNYANT
                     VSITRLNYNDHGPVHMKRVAYNSIKIMSILHEQGFKFSLEKEGAGTYEESLNAVLSAS
                     LIHDIGMSIGRENHEKNALILADPILRRILKKVYPDDFEKQVIVRALAFECISGHMGT
                     LKVHSYEAGVVLIADGCDMEKGRSRIPIMINDEPKPGDIHQYSSAAIEKVRITKGENK
                     PLAINVEMSASVGFFQIEEVLFRKINASTLKPFIELSAGVTGKETKRYLH"
BASE COUNT         3502 a         2730 c         2264 g         3691 t
ORIGIN      
        1 gtgaaaaatc atcaattccg actccattat ccgaattaaa aaataatttg tcaagagaaa
       61 aaataaatta ttttccttct tcgtaaaaac cgcagcttag atgtgctccg tcgcagaacg
      121 gatgattttt tgactgaccg cagcggcaca gacaatagtg ctcttttgat tcgggacatg
      181 agttctgatc gtctttaaat tcgatatagc cctcaaccat caaagggccg ccttttgcaa
      241 ctttaattgc cgggtcacgg tcgaagagat ccttgtaaca aactccgtca acggtgtatg
      301 aaagtgcacc gctaggacat ttttttatgg tttctattat tgcatcctta tttccaagtt
      361 cgggaagtat ccatggcctt ctgtgacggt caaaaactgg tttaagcttc aggcacgagc
      421 cgtcgtgact gcaaattccg aggttgaagt gaactatgat gtcactggct gtataatctt
      481 tgtttctatc cggaattctg tcagattgtt ttttcagatc gagcccttct tttgtatgtg
      541 ttccgtcgca ataaggcatt ttctcagatt ttccacatct gcaaagtgac atcaccggtt
      601 ttgttgaaag agcgtttcca ttacagtcaa acagattttt cagatcgcta accatcaaag
      661 gagaatgaac tgtgcaggtt attctcggct ttttaggtcc gtcacacttt ttttccattg
      721 gtcttctcct taaaaaaagt actggcaaat atagattaaa tcagatgact tgtaaacttt
      781 tagattttca acaacttttc aaatcacctt gcatcagttg atgatctttt ttgacgatat
      841 cgggaacacc gtctttgaaa tttataatat tttttatgaa tgacgcgact tttgaggcat
      901 cacctttcca ttcttcgctg tagaaaatgg tgttttcaag atcaaatgaa agaatgtcag
      961 cttcagtagc ttccgacaag tctacaataa gggtgacagg aagcttgatt gaaaactctt
     1021 tctgcggaga gatgaaccct tttgcctttg aattctcaaa aggatgacca aaccagaaaa
     1081 aatcaccttt tacgcaattt tctacaaacc atttgtcaat tccaataaca agagtctctt
     1141 tcggaatcac gatcaaggga aattacttct tgaaactgct gtgaattttc gacatcacat
     1201 cgtctttggg aaggttttca gaaattgaaa gttctgttga aaggatctcc tcagcttcat
     1261 tgagaagctt atcctcacca tagctcaacc cttttttctt ctttaagaac ataaggtccg
     1321 aaataacgga agcaacatca gtgatctttc cggttttcat tttgtcctga agaaatctgt
     1381 accttttgtt ccagttcatc ttcaaaagat caagatttct gtgtttgaaa cagctgtaaa
     1441 catcttcggt acattccatt tttgtaattg tccgaagccc cattttgtca gcagaatcaa
     1501 ccggaacaaa aacttttgaa ccacttgaca ccatgcttac gacataacat ttgacttctg
     1561 actcacccat cggcctgtgc tcgatagata caacttttcc cacaccgtaa ttgggataaa
     1621 ctgccaagtc gccgggttta aaacatttca taaggaacct ccaagttcaa gaacatgacc
     1681 tattgattat acttattatt atcaagcagt caagcggaaa cataaaaatt cccgcataat
     1741 ttcaggcagt tacttttttg atacatcttg atacctattt tgaagagcat ctaagactga
     1801 tttaataatg caataaacat ctttccatat cacataatcc atttttttta accatgtcat
     1861 ctgacgcttt gcaagatgcc tcgtattctg tgcaattgct ttaatcagct catcttcagt
     1921 agaaattaaa cctttcagaa aaagatccgc ctgggcataa cctattgaat taagcggtct
     1981 gcagtttaaa acattgaagc ccatactgtt aactttcctt acttcatcaa caagacccga
     2041 tttaacgatg gccttagacc taatgtttat tttttcatac aattcttccc tttcgctgct
     2101 aatggcaaaa gaaaaaaact ctcccggata tttttttact ctttctaaac tattccactc
     2161 tgaaagtttt tttcccgtaa gttcaaatac ttcgagacct ctccttattc tctgtctgtc
     2221 attctttgaa gaaatgcgct ctgcccacaa cgggtctgtt tcaaggagtt ttgtatacaa
     2281 ttcttctgtc gagaacaatt tcagcgaatc gttataatcc ttcctcgcca catcatcaag
     2341 ctccggcaca ggggacaggc cgtttataag agcatctaca taaaaatttg ttccgccgac
     2401 aattatcggt accttccctc ttgaattgat atcgttaatt gcatcaagag caagatcagc
     2461 ataaattccg gcacttatct catctttcag atcatgcgta ccgataagat gatggggaac
     2521 tccttccatc tcctcaagag tcggagaagc tgcgccaatg acaagatctc tgtatatctg
     2581 aacagaatca gcacctatga tctctccgtt tatttctttt gcgagttcaa ctgaaactct
     2641 ggtttttcct gatgctgtcg gaccgagaat gaatatgacc attactgtct tttcaccttc
     2701 ttttcaaatt cgttcctgct cattgaaaaa aagaacggtc ttccatgcgg gcaagtcaga
     2761 gattcagaat gattaatgtc ttctatcagt gaatcaatct ccagcgaggt gaggagatca
     2821 tttcttttca ctgagcttcg gcatgcggca gtcgcaagac gggacatgaa aatatcttcc
     2881 ataacccccg aagaaccgta attttcaagc tctccagcca tttccttgac aactcccttc
     2941 cagtcagcat caaaacctat cgcaggaaca gctctgaccg cgactgaact ttcatcgaac
     3001 tgttcgatca gaaatcccgc attgctgaac aagataatat tgctgcttat gatatctttc
     3061 tctatatcag aaagtccgac tattatcggc gcaacaagca tctggctcat tccgctttta
     3121 agttccgacg cttttttcaa agctgtaaaa gttatccttt catgtgatgc gtgctgatca
     3181 aggaatataa ccctgtcagt catttcaatt atctggtaaa ctccgaaaag aaccccgagt
     3241 tttttgtaat ccctgatctt catttcgaaa agattttcat tttccgggct gaagatctcg
     3301 cctgaatagc tttcggggga ctctttttca gaaacagtca tcgttacacc ttcaaatgcc
     3361 tgccctgcct gtgtctcaat tttctcaacc ggccggaaag atttgagcgc accggtaatg
     3421 gagtttgaga tcagctcaga aatgatgaac ggatccctga acctcacttc aagcttggaa
     3481 gggtggacat taatatctac agttccgttc tggacttcaa gaagaataac gacaggagac
     3541 ctgaattccc ctcctacctg ttcgcggaat gtacggagaa tcgcaccgtt tataactctg
     3601 tcgttgacta ttcttccgtt gactgaaaca gcggtgagag aaacgggagc ttcaaaaggt
     3661 gaaggaacat gaactgtcaa tgatatgcca tccctttcca tgtggaatga acggatcatt
     3721 tccctgttta acttccagat atctgatatc ctcacagaaa cagaaggaga agggtttaaa
     3781 gtgaacagat ttcttccgtt gtgagcaaat gaaaagccgg ttttataatt gataatactg
     3841 aatttctgta tcatctcttt aatatgcgcc atctctcttt cgtcactttt cagaaacttc
     3901 tttcttgccg gaatattgtg aaaaaggttg gaacatgata tctccgtccc tttcggcatg
     3961 gagaccggac cgttttcagt tataactccg ttctttgaaa ctatcctgaa accctcacca
     4021 tgagaaatcg cactggaaat cgaaaaatgt gtcactgccg ctattgacgg gatcgcttcg
     4081 cctctgaaac ccattgtcga gattttttca aggtctgcaa tatcatttat tttacttgtt
     4141 gcatgccttt caagagccat gaaaacatca tcttcggtca ttccgcatcc gtcatcttta
     4201 acagttatcc tttttttacc gccattctct atgtcaataa agatgttaag agcccctgca
     4261 tcaatactgt tctcgacaag ctccttgatt atggatgcgg gtctttcaac aacttcccct
     4321 gccgctatct ggtttattgt tttttctgtt aaaagtttta ttctgcccat aatttaagcc
     4381 agatttttaa gtaaatataa taaaagaaat ataactcctg cataaagcac tatcgctccg
     4441 gcaagtatga gtcgctggtt gttcatcgat cttgatatca attctccgtc tataccttcc
     4501 acaactttca ttttacggaa aactgtccgg ataaaaagca taagtcctgc aagagatgaa
     4561 attaatatca tccaccagta aaaattaagt tcaccgctct ttttttcatt gaactccttt
     4621 tcaagaactt ctcctgaaaa tatgagcgga agcggcatga aaaaagagga gttgtaactt
     4681 cttatgaaac tctcctgtgc ttgtaacgga aacttttgga gaaattgtac aagtccagaa
     4741 taatatggat cgtccacttc accttttaaa tccggagatc tggaaaaaac cacataggtt
     4801 gctccgggcg aggaggaatt gaagcttgcc tgtacagttt cacagttcat atatccgtca
     4861 ggcagtcttg ttccggcatt gtttatcgcc acaatgtcag ttcctatcca ccgctctttg
     4921 ctgtaacaat cgacatgaac attgcttgtg tctgaaagtg atgcaagcat catccctgaa
     4981 tcatcggtgt agaatttgaa cattttaccg ttgtacggca cttttgcgat taccgaagaa
     5041 ccgtcactga aagaaatatt aacatttttc tcgtgggtaa atatcgttgt gaacgaaagg
     5101 tatccttctt tcaattcttt tgcaataccg tttattataa tttccctttc cctgcacgga
     5161 ccatctccat ccaaacagta gaaatgaact ttggtttcaa actcaggaac cgctctgaac
     5221 tggtctggaa gcagaaaaac ggtttttgtt cctgaaacgg caggaaatgg aacattttca
     5281 agttcttttg ctgaaatatg tgtccggaca ggagcaggaa ctctgatatt ttccagatcg
     5341 ctgtatctca cagggaattt aacttcactt tttcccacaa cgacagcaat ttctcttaga
     5401 tcaggacgga tatcaataag agatgtcgca gactttctgc cgttaatgaa tatttcaggg
     5461 tttgttacga attcaccatc tcttgaaatg actttcagca gagcttttga gccaggggca
     5521 acttctgcac cgtaaagata aataaaatag tcgtacctat cctgctgggc attgacaaaa
     5581 agaaaaatga aaacaccgac ggaaagaaga ataaatacaa aaagaacaga tgtgaaatct
     5641 atttttcctt ctttcatttt caagactact tcagaatgtc actgagggcg gatcttttca
     5701 gcttttcaat gaaaagatac ggctcaatga tctttaaaag gcggtccgca ttcttctgat
     5761 cggatgaatc aaaaagaaga gataccattt tatctgaaag attgagaacc ccttctatct
     5821 ttccgtttat aaagaccggt aacgacatga aataatcttt ggaataacct tccgtttttt
     5881 tgaacctgga atcattatct atattttcaa cgaaaaccgg ttttcccgta tctatgactg
     5941 tccatgccac actgcccggt ttcggctcta cttcaagccc tataagattt ttttttgttg
     6001 aagcttctat cacaaggttg ccgcttttat taaggagcat catggaacct cttcctgcat
     6061 aaagcatttc aagcattcta gtaaggacat cgttcataaa aacagaaaaa tgggaacacc
     6121 ctttgaaaga gttgtccaca aattccctga atttaaagaa attcttgtat ttatgggcat
     6181 ttgtaaaagc cagtaccgtc tttatgacct gcactgcaac tggcttaaag ccgtccatcg
     6241 gtgcacggaa atatttgtcg gtgtatctta catgatccgc ccagtttgca tgctttgaac
     6301 cggcagatga aaggattaca gtcggaagaa agttgagccg gcacgaattg tgaagttcga
     6361 tcagatcgag tattgaagtg tagttatctt cgacaatgaa gaaaagagca cctcttctat
     6421 gctcattgac tatatcatcg acattgacat tggagtgatt ccacccctca ttccttataa
     6481 tctcagccat actgtcttca aaatccttgt ttctgatagc tgtgcaaacc ttgaaatccc
     6541 tcatggatac cccctgactt tttctgtgcg gagtatatta ttttttcaaa aaactggcaa
     6601 gttttcaaac attgtcatca aactttcatc aaagttgtat cttttgacaa atctgataat
     6661 atcggctgtc attttatggg agtgaacatg aaagaaaaac cttccccgat aaatactatc
     6721 tggctgatga tggtgcttat ttccgttgtt gcggcttcat acaccggaaa gatgtccggg
     6781 ctgaccgcct cggtttttga aagtgcaaaa tcggccgtaa cccttgcaat aggacttgtc
     6841 ggagcaatgg ctctgtggct tggaataatg aaggtgctcg aggaagcagg tgctctgaag
     6901 ttgatcgcaa aagcagtgcg acccgtaatg acccgtcttt tccccgatgt tccgcatgat
     6961 caccctgcaa tgagtgcaat ggtgatgaac atcgccgcaa atatgctcgg actcggcaat
     7021 gcggctacac ctatgggaat taaagcgatg acagagcttg ataaactgaa cagggaaaag
     7081 ggaactgcca caaatgcaat gtgtcttttc cttgcaataa acacttcaag cgtaacactt
     7141 cttccgctcg gagtcataac tgtcagggcc tcagcaggtg caagcgaacc cgcttcgatc
     7201 cttatccctt ccatccttgc aacaatactc tcaacatcat ccgcaattat cgcatcaaag
     7261 cttctgcagt ctaaaaacag cgatgccgca ataagtgaag agaaagccga ggaagaaaaa
     7321 acaccggcaa attacgaaaa acctgaaatc ttcaagattg ttgcggtctc attattcatc
     7381 gcacttgtcg caggcggaat ttacttcagc gcatcagcaa acacagagcc cgtttcatgg
     7441 agaagcttca tgcagcaact ttctgactgg cttatacctg tgatcatggt tgcattcctc
     7501 ctgttcggat atctcaaagg tgtcaaactt tatgaaactg tgactgaagg ggcaaaagag
     7561 ggtttcaatg tggcagtaaa gataattcct ttcatggtcg ctatatttgt tgccgtcgga
     7621 atgttccgtt caagcggagc tcttgacata atggtcaaca ttttcaaccc actgacatca
     7681 ttgatcggaa tgccggcaca agctcttccc atggcattta tcaggcctct ttcaggaagc
     7741 ggagcatttg gcataatgag cgaaatagtt agcaaagagc ccgattcctt cctttcatat
     7801 cttgtttcaa caatgcaggg atctactgaa acaacattct atgttcttgc tgtatatttc
     7861 ggagccgtcg gaataaaaaa tgccagacac gctgtacccg ctgctctcat agctgacggc
     7921 acaggtataa tcggttctgt gataatctgc agaatacttt tttagataat atcagtcaag
     7981 gttgacaatt attgcattta cgatcggttt tttcctggtt atcgcaaaaa gatgccttct
     8041 gacacggatc cttatctcct cttccacatc cttccagaat ggctcatcag ccagttcatc
     8101 cttaaaataa ttgtcaatga tgtctttgac ctccctttct atctttttca ttttcgggac
     8161 agatgctatt cccgaagaaa ctattctcgg cttggaaaga agcgagttcg caagaaagtc
     8221 aactgtcatc atgacagtca tgatccctgc tttagctatt ttcttccgct ccctgatatt
     8281 ctcaatgtct acaagatccg gatcaccgtt cgagacaact ttgacggtaa gatcatattt
     8341 gtgtgaaata acaggccgtt tattcttacg aagggaaagc atctctccgt cagttatcac
     8401 ctgcgcattg aacccgttcc gctcagcaag ctttgcactc agttcaagaa atctgcggtg
     8461 accgtgaaca ggaataatga gatcagggtt tacatcttta aaacactgaa caatatcaaa
     8521 ctgtttagcg tgtcctgaaa catgaatgtc gtcagtattc tcataatata catcggcacc
     8581 tttttcaacg agattgtcta tcattctccc gatcgaaagt tcatttcccg gaatgttctt
     8641 tgatgtgaag aatattgtat caccgaattt caccgaaaca gacttcatca tatcaagact
     8701 cattcttttc ataacacttc tcggttccgc ctgcgatcct gtaacgatga gtgtcagctt
     8761 gttctccggc actctgttga tctttgtctg ttctgtcaga agagatgccg gaagtttcag
     8821 atgacccagc tccgaagcga tccttgtgta tgagcagaca cttctgccca gcaatccgac
     8881 ggtgcggcct tttgaaagtg agatgtcgat tatatttttt atccttgttg tatttgagga
     8941 aaatcccgtc gcaatgactc tgcccggggc attctcaatt atctgggtga tattcggaat
     9001 tattgaactt tcactttctg aatgtcctgt attctcgata tttgtgctgt cagaaaggag
     9061 aacatcaact tttttaggga ctctgccttt aaaaggggat cccttttttc cgtcatattt
     9121 aaaatcacct gtatggacga ttgtaattcc gccgacatct attatgagtc ctttcgcttc
     9181 agggattgaa tgaggaacat cgaacatttc aactctgaac ggaccaagat ttatctcggg
     9241 tgtctttttt gaactgaaac tttcaattct attcttatat tttgcatatc ttcctattct
     9301 ttcttccagt atcattttag gaaattttgt catatagaca ggaacattga atttctgaag
     9361 gagataaggg acgcccccta tatgatcttc gtgtccatga gttataacac atcccagaag
     9421 ttcaatatca agttcattta tgatgtcaaa atcgggaatg aaaaaatcat cctccatgaa
     9481 atcatcgttg ccggggaaac ccattccgca atccaccatt atcgcctgat cttcatattt
     9541 gatgagaaga cagttcttcc ccacttcctt cagcccgccg agagccagaa attctacatt
     9601 tgatgacatt ttattctccg gaaatattgt tattttcaat tcattgttga cataatatcg
     9661 gcatattttt cctgaacaaa gactgtcctg gctttatcca tgacctcttc aagctgagcg
     9721 gttcttgatg ccgaaacatt tctgaagccg cctgcaatat caaaaggtgc agaccacacc
     9781 ctttcgtatg tgaactgcat cggatcgaca ctcttgccgt caagaagaac ggtaaaatga
     9841 agatggggag cagtcgacct gccggaattt cctgctcctg ctataacttc gcctttctgc
     9901 actgtgtatc cgcttaatac aagaaagcgg tttaaatgac cgtaaacagt tttcattcca
     9961 ttgccgtgtt ccagaactat tgtgtttccc aaccccccct ttttacctac aaaggaaact
    10021 gttccagcat cagccgctct gaccggagta tcaatttttg cgattatatc cactccatta
    10081 tggaattttt ttcttttatt gaacggatcc gatctatatc cgaaaaaact ggaaattctg
    10141 ccgctgtcga tcggggatct gaactgaccg ggaggagaaa gcatcattcc atgacttgca
    10201 acaaaataat caccgtaagt tgcatctctg aatgccgtca ttgtaaaatc gcctatggtt
    10261 gaacttctta ccgacagccc cagaatgccg aaagcatgaa caagaatatc gtgatcgaat
    10321 atccctttta ccatgaacga tacagaatca cccggcatca gcttgtcaag aataccccag
    10381 tcccatacca accgctctct tgccatttca taaaactgcg gatcatcctt tgcaagagat
    10441 tcctttattt taaaatattt gacgactgtt tttacctcca gagatatttc ataagtctct
    10501 ttctcgaaat tacgaccttt ccttataaac gatatctttt cgccgataat tctgttcttg
    10561 tagtggtgaa gattgtatga aagaaccttt tcgccaagag tgctgacaga aaaaatatct
    10621 ccagccataa aacggttcag accggcattc tttctgtaaa gctgttttgc agttttccag
    10681 ttctctgttc cgatcatctc ttcaaacatg tcgtcatatt ttccttcggg aacctgcaca
    10741 taggttgttt ctatcttcct tttactgcct gcgctactcc aggatacatg cagatttaca
    10801 tcttttctaa agtgaagatc atagacaatt gccgagacaa taacggcaac cgatattgta
    10861 agtgccagaa accattttac attcattaaa aaactgctat tgaacaacaa aacacacata
    10921 ttttaatcat tatttgaatt atgtcaaaag caaaacttca gcttcagttg acacagaaat
    10981 atttacagta taatcttgtc ccatgatttt ttgtgaggta aataaatgac aaaatcaccg
    11041 aaagaagcag gaacagaaaa aagaattctt gaaaaactta agggaaaacc gcttattctg
    11101 gcaaaagaga tattcagcga tcctgagata atccaccttc agaactatgc caacaccgtt
    11161 tcgatcaccc gtcttaatta caacgatcac ggcccggttc acatgaaaag agttgcatat
    11221 aattctataa aaataatgtc gatcctgcat gaacagggat ttaaattctc tcttgaaaaa
    11281 gaaggagccg gtacttatga agagtcgctt aacgctgttc tttcggcttc tttaatccac
    11341 gatatcggca tgtcgatcgg aagagaaaat cacgaaaaga acgctctgat cctcgctgac
    11401 ccgatattaa gaaggatatt aaaaaaagtt tatcctgatg attttgaaaa acaggttatc
    11461 gtgcgggcac tcgcatttga atgcatcagc ggacacatgg gaactctcaa ggttcattcc
    11521 tatgaagccg gcgtagttct tatcgctgac ggatgtgaca tggaaaaagg acggagccgt
    11581 attcccataa tgataaacga tgagccgaaa ccgggcgaca ttcaccagta ttcttcagcc
    11641 gcaattgaga aagtgcgcat cacaaaaggg gaaaacaaac cacttgcgat aaatgtcgaa
    11701 atgagtgcat ctgtcggctt tttccaaatc gaggaagtcc ttttcagaaa gatcaacgca
    11761 agcacactca aaccattcat cgaactctca gccggagtca ccggaaaaga gacaaaaagg
    11821 tatctgcact gatctttttt gattcgcgaa gtgacaaatt attttacgca acggacaccg
    11881 tatttgatgc tattggagac gtagcccaca tgcccagctt tgaaactcac ataacatgcg
    11941 atgtatttgc gatctgatag ctccgacgac gaccaaaaca aatcagtatc tctaaaaacc
    12001 gaatattcgc cagatgtgtc atttttacac ccacgacatg aataactcca acaatcggaa
    12061 gataaacaac tgtcagtgac tccacattca ccacctgttt cagtagtcgg acagttctga
    12121 ataagtgttc tgagttctga aattgcagga agtctgccac cgaggttttc acaataagtt
    12181 attgcat
//
LOCUS       DAOOYB010000072        12175 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_5447_length_12175_cov_9.473845, whole genome shotgun sequence.
ACCESSION   DAOOYB010000072 DAOOYB010000000
VERSION     DAOOYB010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12175
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5447_length_12175_cov_9.473845"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            187..453
                     /locus_tag="PL056_05795"
     CDS             187..453
                     /locus_tag="PL056_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005993393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PxxKW family cysteine-rich protein"
                     /protein_id="HPM46562.1"
                     /translation="MAIKGKSAGGNEKYAKITKLPIDERCEGCERIEEVESKKFCKKY
                     ADPSFHWENDQTCSFATHIKREIKIVKKVVNPLKASKRAAAKKK"
     gene            466..2064
                     /locus_tag="PL056_05800"
     CDS             466..2064
                     /locus_tag="PL056_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46563.1"
                     /translation="MEKFYFIQYPEQIFPLKQDIEDIIPVIPVYEDLADFGRIISSIK
                     RWGRPAAVFYERTLYPLKQFLEKSGEFDSVFTIDADVVKALRETGFNGKIYLFSNFRM
                     LVHNGFFNDFRVIPVVSGKANYDIAKNKGLEPVYFVSDKICVADFGLCISRKSDPQCF
                     YNCDNRCREKSISKQGLEFPLSMKPETIYPENTSYLVFSEKLQFPNVKQGELRDQVLE
                     RSWNYRYPLGIEILKPGKTGNGKNYTFPLDRKSAKLFEKGLIEFYGYSGSIYKGRWIK
                     SAKAKLSADDKLSVSIGETYQNVLMVQRYADDEKDLLKRARHFIFTLPEKKLLDKIEE
                     EPESIEPVKPGPVRKYSGKTKLTLITDDIKKFHTFKGRDIERKVLIYKRGVPAGFPEF
                     LFIAGAINDYDFEDISHMRKLKGIVVPDGIMKAAFERLFPEKEIVLHPMSYYDPSKSP
                     SFLSASTTIFVSRFKSEKRNAYGWNDINIFLRNQPGFTEFISRKKLSRHGGRKELWVN
                     IADITDDALKYLKSQAELIVRKVR"
     gene            complement(2102..3256)
                     /locus_tag="PL056_05805"
     CDS             complement(2102..3256)
                     /locus_tag="PL056_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HPM46564.1"
                     /translation="MTFNFFATCPRGLEVPLINELSEISAADIKKDDGGVHFSGDISV
                     CYKANLFVRTAGRVYWKVSSSEYRNEQDIYDLAYLIPWHKWFDVSRTIRVSMVGIRCP
                     LKSLNFAGLKIKDAICDRFRKECGSRPDVDPNNPDIRIHGFVTEKELVISIDTSGDTL
                     FKRGFREETNAAPIRENLAAGILYLAGWKPGIPLLDPMCGSGTFLIEAAQMSLNIAPG
                     ISRKFAFEKFKDFDRDLWLKICEKAVEEELDVTELPIFGSDISADAVEMAKTNLNNAG
                     LLEAVTLNCSDILDISAPADNGLIVANLPYGERMGEINELRDLYPKIGDTLKKNFSGW
                     NAYLLSSDLQMPKFIRLSVSKRTPLFNGQLECRLFEYKMVSGSNRSKKDQ"
     gene            complement(3264..4451)
                     /locus_tag="PL056_05810"
     CDS             complement(3264..4451)
                     /locus_tag="PL056_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46565.1"
                     /translation="MINKFITVLLTAAMFLAIFSACEETDFKGLYTPTKPPEIGDKRT
                     YECKGKPGGEAYSWNSVSEYEQTWNGSSWEPVDDPDTEYGDKFYPSSTSCQYTCATCY
                     TWNYSECVSNPNCVTRTYYCQPKPEGEEYEWNTVSEYKQESTDQGASYYPSDDPSTEY
                     NDSPSTTSCQFKCAQGFIWNGSICTKAATGSRTYECPDPLPDTNGAYVWNSVQSYNQE
                     TYDDGVTWNPPDDPETEYNEEGSVTSCRFKCNTGYSWNGSECVYVNWESAVGYQWLKA
                     PNGMTYEDANTFCSNNGGYIPAIDQLKQLIQNCSQTVYGGTCTATNSCVSWSSCRNAD
                     CDGCTMQSSGYYSVFGDESWFWSSTQLSDQEAAAYTINFDTGAINMSVINSTYTGNVR
                     CIK"
     gene            complement(4463..6211)
                     /locus_tag="PL056_05815"
     CDS             complement(4463..6211)
                     /locus_tag="PL056_05815"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HPM46566.1"
                     /translation="MRKLKLLFLTFVFLIIPLISGAAEKDRLAVMDIQDSENIFDAKT
                     QIKFTDYIFTKFQITGVYWMIPKADRDTALEQAIADTAEGSRKQCVDEKCQISLTAQL
                     QANFLINTEIKKLYEGTCQISIRKFDVEKRAGVDAWEEKFDCTDKGIYGAIDSFNFTG
                     KKSAFQAGRISEEALNMNVEAEDGTIVKIVTEPTEAIVLVDGIMICQKTPCSKMIAFG
                     KHQVTIQKENYVPHQKEYDVKAGLALNLILEPNFGWVTINSEPSGMEIELDGKDLGVT
                     PISKRIIETGGHQAQTKNKCHYNEMEKFVIKRGEDKVITFKMNPRESALKVTAKDEQD
                     NDLEAEVMVDGNPVGKTPKTWKVPLCSDNIYVKADVGEFTQKLSLTEGEIKVVDAVLK
                     KAVVYTPPPASTAPAYNNYNYDYSSRRSGSGKFNLHGLMSIPVGGVFDKAFYIGIDFG
                     LEIGVGSGSKADYHRFFSWSILARMGIVSDFNFDKGFLGMRSFDFKLNTKLNFWYLAG
                     GGPQVEALLGTTGYPNKYLVGVSMWAINLPIYLWTDDRSGFNITLLNVDMGWDYNADY
                     KKGQIVFYTSFEFMFH"
     gene            complement(6208..6744)
                     /locus_tag="PL056_05820"
     CDS             complement(6208..6744)
                     /locus_tag="PL056_05820"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HPM46567.1"
                     /translation="MKKLFAVVIILLCLFQVYGSNDKKAYNFISYDLNGNFFNLKAFK
                     EKTKAKYYVVDFFSIVCEPCKKALPDWEKEYPELQKKGVEMVVVALPSDKMKAKDEKP
                     LVKEYFETNKVSFQVMYDLFYAVGDKFGVSKTEHEKTTVEVPMVFLLNDEFKIVKKST
                     KHIEIIEEIKKLTSGVKK"
     gene            complement(6762..7586)
                     /locus_tag="PL056_05825"
     CDS             complement(6762..7586)
                     /locus_tag="PL056_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46568.1"
                     /translation="MKNLFRNCVIFVWFFAVFGCGTAPVAKPEWTKQNGHASFNGSVY
                     VVGRSDGKQTEKEALDSAFITALGDLNLYFGVSVNATLEDVESETDGVYSYSLKKNSV
                     ITGAPIKINKFNKTNVFTEKVDGMFNGYVQIAVPVDEAARITKEIKGMTGYKVIPARA
                     GHEDILSDFVRQWSQKKGLKIAGSALPVDDNVSIDEIAKKSDTAYFIVVKGEVEEPKQ
                     DGKVFITKVKVIIRQFSLVEGRELNTIVEEMKWGEYTPEEAVDKGMKKLVQKMLAQ"
     gene            7759..8094
                     /locus_tag="PL056_05830"
     CDS             7759..8094
                     /locus_tag="PL056_05830"
                     /inference="COORDINATES: protein motif:HMM:NF013209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HPM46569.1"
                     /translation="MDLKNERIVEIGGILTGKIRVSIIRKLLQGPGIVTDIVDELQLN
                     QAVVSKQLGILKNYGIIKCCPSGRCREYCISDPEMMRTLLDSMNKMAEICEKTAKLQN
                     REINGGKNA"
     gene            8087..8431
                     /locus_tag="PL056_05835"
     CDS             8087..8431
                     /locus_tag="PL056_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HPM46570.1"
                     /translation="MLRTNLKHIETVEEFKNLIESGKKTVVVCGRMGPMCIPVYDVME
                     SFESKYPDINFRDMEFDSNVAIQTIRSLPEVRNFAGLPFTVYFRDGKVAAATSSIQNK
                     QQVKEILDTKLV"
     gene            8447..9376
                     /locus_tag="PL056_05840"
     CDS             8447..9376
                     /locus_tag="PL056_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010526550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPM46571.1"
                     /translation="MNKLYDVIILGGGPAGLTAAIYLARSRMKTLIIEKGSTGGQMNL
                     TDKVVNYPGIHEASGAEIAFTMRQQAKNFGAEIITQAEITHFDISGKIKSFEIEDEGI
                     FEAPAVIIASGGVPRTLGVPNEDKFKGRGISFCATCDGDFFKDKKIAVIGGGNSALEE
                     AVSLTKFARHVTIIHEFDHFQAHAWAIEDAQKNPGITFLMNQKVTEFLGDEHISGVVS
                     LDKSTGKKNITEIEGVFLFIGYLPNTSMFKDIVPMNEREEIITDELLKTGIEGVFAAG
                     DAREKRYRQVTTAVSDGTIAAMSATEYVAAKYK"
     gene            complement(9390..>9998)
                     /locus_tag="PL056_05845"
     CDS             complement(9390..>9998)
                     /locus_tag="PL056_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005643755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HPM46572.1"
                     /translation="TDLKIGYWLGNSPYKQETWKFLGVLVSAATVGGVIMILNKTYGF
                     TGENALVAPQANAMAAVIEPMMSGQAAPWMLYAAGAFLALTLNMIGVPALAFSLGMFI
                     PLDLNTPILVGGIINHIVTTRSKDEKLNNARSEQGTLIASGFIAGGALMGVTAAIIRF
                     AGFNFVNEHWFESNGAQWLGIVMFLALCGYMIFHTLKAKVEE"
     assembly_gap    9999..10098
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10099..11216)
                     /locus_tag="PL056_05850"
     CDS             complement(<10099..11216)
                     /locus_tag="PL056_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008123242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HPM46573.1"
                     /translation="MSHQQPEKIKGIPDNAFRELKEGEIYQPIMSPDKVYPEINIWSV
                     VWGLVMAVVFSAAAAYLGLKIGQVFEAAIPIAIIAVGVSTVTKRKNALGENVIIQSIG
                     ACSGVIVAGAIFTLPALYILQAKYPDITVSFMQIFLSSLFGGFLGILFLIPFRKYFVA
                     DMHGKYPFPEATATTQVLVSGEKGGNQAKILLIAGLIGGFYDFAIATFGAWAETVSTR
                     IVGAGVALADKYKLVLKLNTGAAVMGLGYIVGLKYAAIICAGSFVGWFVILPVIAYFA
                     EGQTIPVGANIVNLIGTMSPEEIFRNYVRPIGIGGIAMSGMIGIYKSRKIILQAFGLA
                     ANELTGKGESKKIDLRTQRDIPMKIIAIGILVMTVVLFV"
     gene            11400..11657
                     /locus_tag="PL056_05855"
     CDS             11400..11657
                     /locus_tag="PL056_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006889987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HPM46574.1"
                     /translation="MPEISRFLGIVVFMNFKDHNPPHFHAMYGDYQIIVEINTGVVEG
                     KFPKRALSLLMEWYEIHRDELIKNWETLKNKGSYEKITPLE"
     gene            11661..11918
                     /locus_tag="PL056_05860"
     CDS             11661..11918
                     /locus_tag="PL056_05860"
                     /inference="COORDINATES: protein motif:HMM:NF021873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HPM46575.1"
                     /translation="MILNVKKAVYLKDYVLKVTFNNGETRIIDLESAIFNDPLPLFVP
                     LREKDFFQQFEIKLNTICWKNDFDLAPEFLYEISVPADEAA"
BASE COUNT         3542 a         2568 c         2382 g         3583 t
ORIGIN      
        1 tgcaatgtca aaagtaaaaa ccactactgt aaggatatat gacgatgttc cggtcgatcc
       61 gatagtcatt gaaagtgcat atatggtaaa ataaaatcct aaaattttat gcttttattg
      121 acaaccggga aaaactgtgt ttaataaccc cgtttttaag ttcttaacca tttggaggat
      181 gctgaaatgg caattaaggg taaaagtgcc ggcggaaacg aaaaatacgc caaaataaca
      241 aaacttccca tcgatgaacg ctgcgaagga tgtgaaagga ttgaagaagt tgaatcaaaa
      301 aagttctgta aaaaatatgc agatccttca tttcattggg aaaatgacca gacatgttca
      361 tttgccaccc atataaagag agagatcaag atagttaaga aagtcgtcaa tccgcttaaa
      421 gcttccaaga gagcggcagc taaaaagaaa tagcagtatc ctgatttgga aaagttctat
      481 ttcattcaat atccggaaca gatatttcct cttaaacagg acattgaaga tatcattcct
      541 gtaatccccg tttatgaaga tcttgccgat ttcggtagaa taatctcttc aattaaaaga
      601 tggggccgtc ctgctgccgt tttttatgaa agaacccttt accctttgaa gcagtttctt
      661 gaaaaaagcg gtgaattcga ttcagttttt acaattgatg ccgatgtggt caaggcgttg
      721 agagaaaccg gatttaacgg aaaaatttat cttttcagca acttcagaat gctcgttcac
      781 aatggattct tcaatgattt cagagtcatt cctgttgttt ccggcaaagc caactatgat
      841 attgcaaaaa acaaagggct ggaaccggtc tattttgtca gcgataaaat atgtgtcgcc
      901 gatttcggac tgtgtatttc aaggaaaagc gatccgcagt gtttttataa ctgtgacaac
      961 cgctgccgtg aaaaaagtat ttccaaacag ggactcgaat ttccactttc aatgaaaccg
     1021 gaaactattt accccgaaaa tacatcttac cttgtttttt cagagaagct tcagttcccg
     1081 aatgtaaaac agggtgaatt aagagatcag gttcttgaaa gatcgtggaa ttacagatat
     1141 ccgctcggaa ttgagatcct taagccggga aagaccggaa atgggaaaaa ctataccttt
     1201 ccccttgaca gaaaatctgc aaaacttttt gaaaaagggc ttattgaatt ttatggatat
     1261 tccggctcta tctataaagg aaggtggata aaatcggcaa aagcaaaact ttccgcagat
     1321 gacaaactct ctgtttccat tggtgaaaca tatcagaatg tccttatggt gcagcgttat
     1381 gctgatgacg agaaagatct tttaaaaagg gctcgacatt tcattttcac acttcctgaa
     1441 aaaaagctcc ttgataaaat tgaagaagaa ccggaaagca tcgaacctgt taaaccgggg
     1501 cctgtaagaa aatattcagg aaaaacaaaa ctcactctga taactgatga tatcaagaaa
     1561 tttcacacat tcaaaggtcg cgatatcgaa aggaaagttc ttatctataa aaggggcgtc
     1621 cctgccggat ttccggagtt tctttttatt gccggagcca taaacgatta tgattttgaa
     1681 gacatatcac atatgagaaa gctaaaggga atagttgttc cggacggcat aatgaaagcc
     1741 gcttttgaaa gattattccc tgaaaaagag atcgttcttc atcccatgtc ctattatgat
     1801 ccttcaaaat ccccttcttt cctttctgct tcaactacca tttttgtttc aaggttcaaa
     1861 tctgaaaaaa ggaacgccta cggatggaat gacatcaaca tatttttgag gaaccagccg
     1921 ggctttactg agttcatatc aagaaagaaa ctctcaaggc acggtggcag aaaagaactc
     1981 tgggttaaca tcgcagacat caccgatgac gcacttaaat atctaaaatc acaggcagaa
     2041 ttaatcgtca gaaaagtcag gtgacatgcc tggaacccaa aattgatcaa gtaatcgaat
     2101 tctattgatc tttctttgat ctgttgcttc ctgacaccat tttatattca aaaaggcggc
     2161 attcaagctg tccgttaaat aaaggtgtcc gctttgaaac ggaaagacgg ataaatttag
     2221 gcatttgaag gtcggaagaa agaagatatg cgttccagcc cgaaaagttt ttcttgagtg
     2281 tgtctcctat tttgggatag aggtctctga gttcgtttat ctcccccatc ctttcgccgt
     2341 atggaagatt tgcaacgatg agaccgttat cagccggagc tgaaatatca agtatatctg
     2401 aacagttaag tgtcaccgcc tcaagcagtc ctgcattatt aagatttgtt ttagccattt
     2461 caacggcatc agccgaaatg tcactgccga atatcggaag ttcagtcaca tcaagttctt
     2521 cttcaaccgc tttttcacaa attttaagcc agaggtcgcg gtcaaaatcc ttaaatttct
     2581 caaatgcaaa ttttcttgat atcccaggag caatgtttaa agacatctgc gccgcttcga
     2641 tcaggaacgt tccgcttccg cacatcggat caaggagagg aattcccggc ttccagccgg
     2701 caagataaag aatgccggcg gcaagatttt ctcttatcgg tgcggcattg gtctcttcgc
     2761 ggaatcccct cttgaaaagt gtgtctccag atgtatcaat tgaaattacc agctcttttt
     2821 cggtaacaaa cccgtgaatt ctgatatcag gattattggg atccacatcg ggtcttgagc
     2881 cgcactcctt tctgaacctg tcgcatatcg catctttgat cttaagtcct gcaaaattga
     2941 gacttttcag aggacatctg atcccgacca tgctgactct gatcgtcctt gaaacatcaa
     3001 accacttatg ccacggaatt aaatatgcaa gatcataaat atcctgctcg tttctgtatt
     3061 ctgacgatga aactttccaa tagaccctgc cggcagtgcg gacaaagaga ttcgctttgt
     3121 aacatactga tatgtctcct gaaaaatgaa caccaccgtc atctttttta atatccgctg
     3181 cagaaatttc tgaaagttca ttgattaggg gaacttcaag acctctggga catgttgcaa
     3241 agaaattaaa tgtcattatg aaactatttt atgcaacgga cattaccagt ataagtacta
     3301 tttataacac tcatattgat tgcaccggta tcaaaattta tagtatatgc tgctgcctcc
     3361 tgatctgaca actgagttga agaccagaac cagctttcat caccaaaaac agaataatat
     3421 ccagaacttt gcatagtaca tccatcacaa tctgcatttc tgcatgatga ccatgaaaca
     3481 catgaattag tcgcagtgca tgttccaccg taaactgttt ggctacaatt ttgaataagc
     3541 tgtttaagct ggtctattgc aggaatatat ccaccgttat ttgagcagaa tgtgttagca
     3601 tcctcataag tcattccatt gggagcttta agccactggt accctactgc agattcccag
     3661 ttcacataaa cacattccga tccgttccat gaatatcctg tattgcactt gaaacggcag
     3721 cttgttacgc ttccctcttc gttatattct gtttccggat catccggcgg attccaagtc
     3781 acaccgtcat cataagtttc ctgattataa ctctgaactg aattccagac atatgctcca
     3841 tttgtatcgg gaagaggatc ggggcactca taagttcttg aacctgtagc cgccttggta
     3901 catattgaac cgttccagat gaaaccctga gcacatttga actgacagct tgtggttgat
     3961 ggtgaatcgt tgtattccgt tgaaggatcg tctgatggat aatatgacgc tccctgatca
     4021 gttgattcct gcttatattc ggagacagta ttccactcat actcctctcc ttccggtttt
     4081 ggctggcagt aataagttct ggttacacaa ttcggattgg aaacacattc gctgtaattc
     4141 caagtgtagc atgttgcaca tgtatactga cagcttgtac tacttggata gaacttatcg
     4201 ccgtattcag tgtccgggtc atcaaccggc tcccatgatg aaccgttcca tgtctgctca
     4261 tattctgaaa cagagttcca cgagtaagct tcaccgccgg gtttgccctt gcattcataa
     4321 gttcttttat ctcctatttc cggtggctta gttggtgtat aaagtccttt gaaatcggtt
     4381 tcttcacatg cggagaaaat tgccagaaac atcgctgctg tcaacagtac cgttataaat
     4441 ttatttatca tttttctcct ccttaatgaa acatgaattc aaaacttgtg tagaaaacta
     4501 tctgcccttt cttgtagtca gcattataat cccagcccat gtcaacattc agaagtgtta
     4561 tgttgaaacc cgacctgtca tctgtccaca gataaatcgg cagattaatc gcccacatgg
     4621 aaacacccac aagatactta tttggataac ctgttgttcc aagaagagct tcgacctgag
     4681 gaccgcctcc cgcaagatac cagaaattga gcttggtgtt aagtttgaaa tcaaacgatc
     4741 tcattccgag aaatccttta tcaaagttga aatcagatac aattcccatt ctggcaagaa
     4801 ttgaccacga gaaaaaacgg tggtaatcag ctttgctacc gctacccaca ccgatctcaa
     4861 gaccgaaatc aataccgata tagaacgcct tgtcaaaaac accgcctacg ggaatgctca
     4921 taagtccgtg tagattgaac tttccagacc cgcttcttct tgaactgtaa tcatagttat
     4981 agttgttgta tgccggagct gttgatgctg gaggaggagt ataaactact gcttttttaa
     5041 gaacagcatc aactaccttg atctctcctt ctgtgagtga aagtttctgc gtgaattccc
     5101 ccacatcggc tttgacatat atattgtcac tgcataacgg aactttccat gttttgggtg
     5161 tttttcctac aggatttccg tcaaccatga cttcagcttc aagatcgttg tcctgttcat
     5221 ctttggctgt aactttgagt gctgattctc ttggattcat cttaaaagtt atgaccttgt
     5281 cttcgcctct tttgatgacg aatttttcca tttcattgta gtggcatttg ttttttgtct
     5341 gtgcctgatg acctcctgtc tcaatgatcc tttttgagat tggtgtcaca cccaggtctt
     5401 tcccgtcaag ctcgatttcc attcccgacg gttcagaatt tattgtcacc catccaaaat
     5461 tcggctcaag tatcagattc agggcaagac cggctttcac atcatattcc ttctggtgag
     5521 gaacataatt ttccttctgg atcgttacct gatgctttcc gaaagctatc attttggaac
     5581 acggggtttt ctgacatatc attatcccgt caacaagaac tatcgcctca gtcggttcag
     5641 taactatctt gacaattgtg ccgtcttccg cttcgacatt catgttgaga gcttcttcag
     5701 atattcttcc tgcctggaaa gcactctttt tgccggtaaa attaaagctg tcgatcgcac
     5761 cgtaaatgcc tttgtctgta caatcgaact tttcctccca tgcatcaacg ccggcccttt
     5821 tttcaacatc gaattttctt atgcttatct ggcaagtccc ttcgtaaagt ttcttgatct
     5881 cggtatttat aagaaaattt gcctgaagct gtgcagttag agatatctga catttttcat
     5941 ctacacactg ttttctgctc ccttccgctg tgtcggcaat tgcctgttcg agagcagtat
     6001 ccctgtcggc tttcgggatc atccagtaga caccggttat ctggaatttt gtaaagatat
     6061 aatctgtaaa tttaatttga gtcttggcgt caaaaatatt ttcgctgtcc tgaatatcca
     6121 ttacggcaag acggtccttt tcagccgcac cggaaattaa cggaatgatc agaaaaacaa
     6181 atgtcagaaa cagtaattta agttttctca ctttttgacc cccgatgtca gttttttaat
     6241 ttcttcaata atttcaatat gtttagttga tttcttaaca attttgaact cgtcgttaag
     6301 cagaaatacc atcggaactt cgactgtcgt cttttcatgc tccgtttttg aaacaccgaa
     6361 cttgtcaccg accgcataaa aaagatcgta cataacctgg aaacttacct tgttggtttc
     6421 aaaatattct ttcacaagcg gtttttcatc tttggctttc attttatccg aaggaagggc
     6481 aacaacgacc atttcgacac cttttttctg aagttccggg tattctttct cccagtcggg
     6541 aagtgccttc ttgcaaggtt cacagactat gctgaaaaaa tcgacgacat aatactttgc
     6601 tttcgttttt tctttaaatg ctttcaaatt gaaaaaattt ccgttgagat catagctgat
     6661 aaaattgtac gcttttttat cattgctgcc gtagacctga aacagacaga gcagaatgat
     6721 caccaccgca aaaagttttt tcatcgtttc ctccagttta tttattgtgc cagcattttc
     6781 tgtacaagtt tcttcatccc cttatcaact gcttcttccg gtgtatattc accccatttc
     6841 atctcttcaa ctatcgtatt aagctctctt ccttcaacaa gcgagaactg tctgatgatc
     6901 actttcactt ttgtaataaa taccttgccg tcctgtttcg gctcttcaac ttcacctttt
     6961 acgacaatga aatatgctgt gtcagatttt tttgcgatct catcaattga cacattgtca
     7021 tctacgggaa gagccgagcc ggctattttc aatccttttt tctgtgacca ctgtctcaca
     7081 aaatcgctca gtatgtcctc atgaccggct cttgcaggaa taactttata tccagtcatg
     7141 ccctttatct cttttgtgat ccttgccgcc tcatcgacag gaacagctat ctgaacatag
     7201 ccgttgaaca ttccatcaac tttttcggtg aacacattgg ttttattgaa tttattgatc
     7261 ttaatcggag ccccggtgat cacactgttc tttttaagcg agtatgaata gacaccgtct
     7321 gtctctgact caacatcttc cagagtcgcg ttgacagaaa ccccgaaata tagattgaga
     7381 tcgccaagag ccgtaatgaa tgctgagtcc aacgcctctt tttcagtctg tttaccgtca
     7441 gaacggccga ctacataaac agagccgtta aagctcgcat gtccgttctg tttggtccat
     7501 tccggctttg caacaggtgc cgttccgcat ccaaacacag caaaaaacca cacaaaaatc
     7561 acacaatttc tgaaaagatt tttcaagagt ccactccgcg aaaagaaatt taatggtgat
     7621 aattatacta atatagttaa aaaatcaagg ttttgccttg tcagattttg gtttgacagg
     7681 ttttgtaatt cagtttgccc ccttgacata ttccgttttg gatatatata catgtcatgt
     7741 tcatttcgga ggtgtaacat ggatttaaaa aatgaaagga tagtcgagat cggaggaatt
     7801 ttaacgggaa aaatcagggt gtcgatcatc cgcaaacttc ttcaagggcc gggaatagtt
     7861 acagatattg ttgatgaact gcaattaaat caagctgttg tttcaaaaca gctcgggatt
     7921 ttaaaaaatt acggcattat aaaatgctgt ccttcaggta gatgcagaga atactgcatt
     7981 tcagatccgg aaatgatgag aacacttctt gattcaatga acaaaatggc cgaaatatgt
     8041 gaaaaaacag caaaacttca gaacagagag atcaatggag ggaaaaatgc ttagaacaaa
     8101 tctaaaacac attgaaacag ttgaagaatt taagaatctt atcgagagcg gtaaaaaaac
     8161 cgtggtagta tgcggcagaa tgggccccat gtgcatccct gtatatgatg tcatggaatc
     8221 ttttgaatca aaatatccgg atatcaactt cagggatatg gaatttgatt caaatgttgc
     8281 tattcaaacc atcagatccc ttcctgaagt cagaaatttt gcaggacttc catttactgt
     8341 ttatttcagg gacggcaaag ttgccgctgc gacaagcagt attcagaaca aacagcaggt
     8401 aaaggagatc cttgacacta aacttgtgta ggaggagtgc cgaaaaatga ataaactgta
     8461 tgatgtgatc attctgggcg ggggtcctgc gggacttact gcggcaattt acctcgcccg
     8521 ttcaagaatg aaaactctga taatagaaaa aggaagtacc ggtggacaga tgaatctgac
     8581 ggacaaggtt gtcaactatc ctgggataca cgaagcgagc ggcgctgaaa tagcattcac
     8641 aatgcgacag caggcaaaaa atttcggtgc agagatcata actcaggcgg aaataactca
     8701 ttttgatatt tcaggaaaga taaaatcatt tgagatcgaa gacgaaggca ttttcgaagc
     8761 accggcagtt atcatagcaa gcgggggcgt tccgagaaca cttggtgttc ctaacgagga
     8821 taaattcaaa ggcaggggaa tttcattctg tgcgacttgc gatggcgatt tcttcaaaga
     8881 caaaaagatt gctgttatcg gaggaggaaa ctctgcattg gaagaagctg tttctcttac
     8941 taagtttgcc cggcatgtaa caataattca tgaatttgac cactttcagg cccatgcatg
     9001 ggctattgaa gacgcacaga aaaaccccgg gatcacattt cttatgaacc agaaagttac
     9061 agaatttctt ggagatgagc atatcagtgg tgttgtttcg ctcgataaat caactgggaa
     9121 aaagaatatt actgaaattg aaggggtgtt tctatttatc ggatatcttc cgaacacatc
     9181 aatgttcaaa gacattgttc caatgaacga acgggaagag atcataacag acgaactcct
     9241 caaaacaggg attgaaggtg ttttcgccgc gggagatgcc agagagaaaa gataccgtca
     9301 ggtcacaaca gccgtttcag acggaactat agctgcaatg agcgcaactg aatatgtcgc
     9361 ggccaaatac aaatagatcc tgttgaattt tattcttcga cttttgcctt gagtgtgtga
     9421 aaaatcatat atccgcagag tgcaaggaac atcactattc cgagccactg tgcgccgttt
     9481 gattcaaacc agtgttcgtt gacgaagtta aagccggcaa aacggattat cgccgctgta
     9541 actcccatta aggcaccgcc tgcaataaag cccgatgcga taagtgttcc ctgctcgctt
     9601 cttgcattgt taagtttttc gtcctttgaa cgggttgtaa cgatgtggtt gattattccg
     9661 ccgacaagta tcggtgtgtt aagatcaagc ggaatgaaca tgcccagaga gaaagcaagt
     9721 gccggaactc cgatcatatt gagagttagc gcaagaaaag ctccagccgc ataaagcatc
     9781 cacggagccg cctgtccgct catcatcggt tcaatgaccg cagccattgc attcgcctga
     9841 ggagccacaa gagcattttc gccggtaaaa ccgtatgttt tgttgagtat cattattact
     9901 ccgcctactg ttgcggcaga aacgagaact ccgaggaact tccatgtttc ctgcttgtat
     9961 ggagaatttc caagccagta acctattttc agatctgtnn nnnnnnnnnn nnnnnnnnnn
    10021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10081 nnnnnnnnnn nnnnnnnnac aaaaagaact actgtcatca caagaattcc gatagctatg
    10141 atcttcatcg gaatgtcccg ctgggtacgg agatctatct tttttgattc tccttttcca
    10201 gtgagttcgt ttgccgcaag accgaatgcc tgtaatatga tctttctgct cttataaatt
    10261 ccaatcattc cgctcattgc gataccgccg ataccgatag gacggacata gttcctgaat
    10321 atttcttccg gagacatagt accgataaga ttaacgatat tggctccgac aggaattgtc
    10381 tgaccttcag caaagtatgc aatgactgga agtattacaa accagccgac aaaagaacct
    10441 gcacagatta ttgccgcata tttgagtccc acgatataac caagacccat gaccgcggca
    10501 cccgtgttga gcttaagaac cagtttatat ttgtctgcga gggcaactcc tgcaccgact
    10561 attcttgttg aaacagtttc tgcccatgct ccgaatgttg caatcgcaaa gtcataaaaa
    10621 ccgccgataa gtcctgcaat taaaaggatc ttcgcctgat ttccaccctt ttctccgctt
    10681 acaagcactt gtgttgtggc agttgcttca gggaaaggat attttccgtg catgtcggcg
    10741 acaaaatatt ttctaaatgg gatcagaaac agaattccca aaaatccgcc aaaaagtgaa
    10801 gaaaggaata tctgcatgaa actcacagta atatcagggt atttcgcctg aagaatgtaa
    10861 agagccggca gagtgaaaat cgctccggca acaataacgc ccgaacatgc tccgattgac
    10921 tggataatta cattttcacc cagtgcattc tttctttttg tgacagtgga aacacctacc
    10981 gcaatgatcg caataggaat tgccgcttca aaaacctgac cgatcttaag cccgagatat
    11041 gccgccgcgg cagaaaaaac gaccgccatg acaagtcccc agaccactga ccagatgttg
    11101 atctccggat agaccttatc cggtgacatg atcggctgat aaatttcgcc ttccttaagc
    11161 tctctgaacg cattgtctgg aatgcccttg atcttttccg gctgctgatg tgacatgata
    11221 actccttcgt taaagttaaa tcaacaaaag aatttaagag aaaactggtt ttatgtcaac
    11281 attatggaac acaaatcctt ggcacttgct tttaaaaaaa gactgcaact gaaaatgcgg
    11341 ttgacatagc atcataatta tataaaatta gttagttttt tgagttatgg agttgtaaga
    11401 tgccggaaat atccagattc cttggaatag ttgtttttat gaactttaaa gatcacaatc
    11461 cacctcactt ccatgctatg tatggtgatt atcaaattat tgttgaaatt aataccgggg
    11521 ttgttgaagg caaatttccc aagagagcac tttctcttct gatggaatgg tatgaaatcc
    11581 acagagacga attgattaag aattgggaaa ctctgaaaaa caaaggttct tatgaaaaaa
    11641 taacacctct ggagtaaaca atgatactta atgttaaaaa ggccgtttat ttaaaggatt
    11701 atgttttgaa agttactttt aataacggag aaactcgaat aattgacctt gagtcggcaa
    11761 ttttcaacga tcctcttcct ttgtttgtgc cgctcaggga aaaggatttt tttcagcagt
    11821 ttgaaattaa acttaataca atttgctgga aaaatgattt tgatctcgct cctgaatttc
    11881 tttatgagat cagtgttccc gctgatgaag ccgcctaagc tgaatttttc aatcgttcct
    11941 atacacacag tttccctcta aatttgccag atgcaatagt ttaatgtata atcggtttta
    12001 agatttttga cagtcgtttt tattgcgagg ttgatgatga taaagcggat tttgttaatg
    12061 attccattgg tttttttgtt ttcttcgatt ttttctgcgg aaaaagaccg tcttgcggtt
    12121 atggatgttg ctgatgaagc gaaactgttt aacaagacaa caattgccaa tgtta
//
LOCUS       DAOOYB010000073        12143 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_5467_length_12143_cov_13.291198, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000073 DAOOYB010000000
VERSION     DAOOYB010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12143
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5467_length_12143_cov_13.291198"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2745)
                     /locus_tag="PL056_05865"
     CDS             complement(<1..2745)
                     /locus_tag="PL056_05865"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HPM46576.1"
                     /translation="MKKLFGLLFSVFFMLTINAAKFDIPLNLFDSGFSNKIALDKAVV
                     DTESRYISIYMTVDKSWIEKLDDLNLEALNRFYLNFLINKKIDFKGIAFFVRTNNLEA
                     YQHILKFLPVLPPVPKKEGESAPQTKSLVPEKKYGNIEGGLTGKTIFTSPGHGWVYDT
                     DNGTWNTQRGINLGMREDDSNAEIVSYFLVPYLQNAGALVFSARETDRNESMVIVDDA
                     DGSSFPESGTYEEGGSWTETTSGKGYGRTAFPIEIESNVMENGKYRYATAGTGAWAKW
                     TPNIPEDGNYHVYVSYKTFTDRAPDAVYTVNHAGGETEVTVSQKHHGSTWVDLGQFYF
                     KKGTGGSVTLNKNDDAESGTIIIADAVRIGGGNAILKRGTSTSGKPRWEEAAKVHAQF
                     LGAPSTVYQSSVNDRSADVTARSRWTAWENEAGVDDSIYISWHSNAFNGSSKGISTYI
                     YSSDEPGSTYVTTEAQAGSVELGELVHNRIFNAVKSLFDSSWKQIGNGMYSAYFGELN
                     PTYNNEMPACLVELAFHDNELDAAMLKNPKFRNIAARAAYQAIVKYFADRDDYDPVYL
                     PSHPRNLRTETNDDGSVTLFWDEPESDENFYLGHPADGYLVQMSTDGKSFDTGTDVGN
                     VTQFTVEDPQPGQPLYFRIIAYNDGGVSFPSVVAGAVPQKTGAQILIVDGFERVDQNT
                     PRFAVGTNGSNRYMLEFINSYSYVIHYLEVFEELGFSADFTQKTNIGSIDLSKYDAVI
                     WFAGEQSSNDTTFSISEQGYIAQYISDGGSFFVSGSEIGWDLVEKGNSADITFFNDVL
                     NTDYINDSSNLYTFKGTGDFSTISGLFDDGTYIYQPNFPDVIEPFNSNGESFLFYDND
                     ETLGAGVITIDTKKVAILGFPFETILEKNMKTGLMEKVFEKFEITPKEIPDDEEPDDG
                     E"
     gene            2861..3904
                     /locus_tag="PL056_05870"
     CDS             2861..3904
                     /locus_tag="PL056_05870"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HPM46577.1"
                     /translation="MAESTKKPLYKTIAQDLKRRIDEGEFDKEGRLPAIRDLAKEYLT
                     TAVTMTNAIRELDEEGLLKKVLGSGIYLKKRAVDPFFPVDDLKKIFISVLENEGAEAF
                     EYGDSSGFFGLKEVVKERALKKLNNMKDREILITSGSQQALHLIFSSMLKVGDWVLVE
                     EPTYPAAIRILKEKGAQIETVKMKDGGPDIKEITRIFDSRPLKMAYLMPRFHTPTGFS
                     YDEKVKKTVSDLSKKHGVYIVEDNSYGELDFETHKEVPLTVEAVLHIYLSSFSKTFLS
                     GARIGTCICGNEVYEKLTKAKLDHDLSSPLFFQMVLAKYIMSGHYDTRLKELNKLSKN
                     KFDEIRQGMKVSS"
     gene            complement(3894..4313)
                     /locus_tag="PL056_05875"
     CDS             complement(3894..4313)
                     /locus_tag="PL056_05875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HPM46578.1"
                     /translation="MQKNLNITEKTNVVIHSLALIAAHPEMDAVSVKFIAKELAVSES
                     YLAKVLQPIVKNGLLDSSRGAKGGYVLKKKPEKIKMLDLILLLEGNFPHSQCLFDRPR
                     CSDESCPFRRLSVKVKETIIKELDGFTIADVAKNFKN"
     gene            complement(4338..5984)
                     /gene="hcp"
                     /locus_tag="PL056_05880"
                     /gene_synonym="priS"
     CDS             complement(4338..5984)
                     /gene="hcp"
                     /locus_tag="PL056_05880"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010939296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HPM46579.1"
                     /translation="MFCHQCQETAMNRGCSAKQGVCGKSAETANLQDLFIWSLKGISF
                     WAVRAKKHGWYDEKTAFFVDKGLFKTITNADFNKQDFIADIYEAVKLREKIRTFMNGK
                     ETVTAIPECAEWVPGNDDDIYAKAALGAGGWLEIENEDIRSLKSLVLYGLKGISAYLE
                     HGYQIKKQGREIFEYLMYALDQIAGNEITADELVALTMKTGEWGVTVMKYLDEANAHY
                     GEQEITEVNLGVRNNPGILISGHDFVDLEELLEQTKGTGVDVYTHSEMLPAHYRPAFK
                     KYDNLVGNYGNAWWLQKEEIKKFNGPVLFTTNCLVPPELDYKERIFTTGAVGFEGLKY
                     IADRPEGGHKDFSEIIEMAKKCQPPVELEKGKIVGGFGHKQTLALADKVIDAVKSGAI
                     KRFIVMAGCDGRHKTRSYYTEVAEKLPKDTVILTAGCAKYRYNKLPLGDIGGIPRVLD
                     AGQCNDSYSLAVIAIKLAEAFELDDINKLPLSFDIAWYEQKAVLVLLALLYLGVKGIR
                     LGPTLPGFLSAGVAKVLVEKFDIKGITDADSDVAAMMEGR"
     gene            complement(6093..7505)
                     /locus_tag="PL056_05885"
     CDS             complement(6093..7505)
                     /locus_tag="PL056_05885"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPM46580.1"
                     /translation="MKKLIKLSILLIIPVLYHFTAFAESMTIGEVIKVALEQNLQLQS
                     EKSEEEYQSYISKSVRGKYLPVLKVSASALLWDEENKTKMDLPVLDAVIQEIVPTLSP
                     DSRQRIQNAVNESDSSGITVHDQHAYKISATIVQPVLSLYGIYNLHNASLKMKKAAEY
                     DTLKIKRELQKDIAKVYFRMLSAIENEKSFDAAIDQIEKTYPVIEALIDEGRVEPNAL
                     LKLKMQRSDFEKGKISASIAVMNSKLILNMIMNRDLDSPLEPVWNGEYFKTFTQKITE
                     NDNAENTVDFLNNIPEIKSTEERINALKSNRHAAVSSFLPELNLVLNYDFQEGFGDMQ
                     PQNQFYAGILFSWNIWEWGSGFYKIKAAQTQTAKTQLNLALLKSGKTVEIRQLQNSLK
                     KALTEIETGEVQVEYAKENLKIEEELFKAGNSTTTELLQAQTSLVKAENDLNIKQAEL
                     CSFYIEFILSTGRDFKDLEI"
     gene            complement(7502..10600)
                     /locus_tag="PL056_05890"
     CDS             complement(7502..10600)
                     /locus_tag="PL056_05890"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPM46581.1"
                     /translation="MRFTEGLIKNKYVVWALVIAAVIFGIQAYFSIPMQLFPDTAPPL
                     INVITAYPGAGAKDVNETLSQKLEEEFASLDGVVKTKATSQDNLSIISVEFDYERDVD
                     LAAVDIQNSISRIKNTLPAGITDPQVLKFSTSDKPVVTIGIISDDLTKARKMSEDVFA
                     PTLQRIQGVAAVDVFGGNKPALIIELNRRDVEAYRIPFQKVVNAVREFNYSMPAGQIR
                     TETTQTMFRLESRAENIEDIRNIPISLPNGNRLLLGEISSIKKGSLDDDARFSIDGRN
                     AIAIQIFKTYDANTVEVVKKSLEKAKELDSQYSSYSFITGEESATFTEISVSNLLSNV
                     WQALLFASIIIFLFLGRIRSSLVTIVSMPLSFGLTFVLMKLFNVEFNMVTLSAIILAV
                     GMVVDSSIVILENITRRMMEEGESPEKAVVNGTSEVGLAVLAGVATTLSVLIPLLFLE
                     GFTSKTFGPLALTLIFAFSSSIAVAFVLVPVLTLYTAGKSKIDTIGEKVAKPFQFLMD
                     MLRNFYTKVLEKALNHRTVTLFAVLILFVVSIIGLQSLGMETLPKMDGGSFSVSLQTP
                     SGSSLEETAKAIGQIESFLTEMPEVEKIQSQSGFEQGMKSMSSFGVQGPTQGSITVTL
                     SPRNERDRSIWEIQSVVRNKLRKIPGIAASTVREIGNTAKATTSAPIVAVITGNEPVV
                     LDRIGNEVVKKLSQVPNVIEPVRSWDFDHKSVAVKVDDQLAVELGISPATVAQTMNMG
                     SNGISAGEFFGYSGNPDPVLIRYSRTDTSEPDDILGFPLFAPGTLHIIPLRAVAKIEE
                     RKGQGVFTRENLISTLEVSAFSEGRALSFVINDVEKSLSDLLLPQGYGITITGEKSDL
                     GESKKELIGAFLIAIIAVYLLLVAQLRSFIQPVTIMISIPLSMIGVFLALFIAGKYIS
                     MPVMVGLILLVGIVVNNAIILIEFINQERERGVKRKKALINAVTLRFRPIMMTSFSTV
                     VGMIPLSLELALGSERFSPLATAIIGGMTAATFLTMIVVPVFYDVFDSMSEKLSKIGA
                     KK"
     gene            complement(10597..11700)
                     /locus_tag="PL056_05895"
     CDS             complement(10597..11700)
                     /locus_tag="PL056_05895"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPM46582.1"
                     /translation="MKNGKIKTIPILISLFLLSTLAFLSCSTEKTEIVRETPVAVKII
                     AVRKGDASKKIRYIGSVHSENEIRILARINGNILALPFKEGMSVKKGAVLSKISAPET
                     GARISKMKEEIAKAQKESEFICDQAEADERLFEKNAVPSIRAETSKKNCENSKLSINS
                     ATSSLDELEIMSSKTVESAPFSGTVLKWFSNPGENIMPGFPILLFGDDSYIVKVFVHE
                     KDISKGIKKGLKVILENNEDQSAESAVSFVSPISAGPGRMYETHIPLKKDLLQNFSHG
                     HSINVSFIVSEKKNSFSVPSNSVLKDGESEYIFLFEDEKIEKRKIVLGISEKGWVTID
                     GDIPENSKVVVGNLEALSSGMTVYPVFLKGEKL"
     gene            complement(11697..>12143)
                     /locus_tag="PL056_05900"
     CDS             complement(11697..>12143)
                     /locus_tag="PL056_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46583.1"
                     /translation="TKNDIVLYIIKSIEHKFFEGSADFSGDPVERLGQFFKKRISVLF
                     KNRNAMNVMFSDQFSKIAGSEGVAIIKEMREKSLKFVHSTLNDAFNAGLLKDGLDPDN
                     LTVLILGSILSIYNSHLYTSLMKERELKKISAGMWETWEKLIRRQS"
BASE COUNT         3435 a         2578 c         2308 g         3822 t
ORIGIN      
        1 ttcaccatca tccggttctt catcatcggg aatctctttt ggagtaattt caaatttctc
       61 aaacactttt tccatgagcc cggttttcat atttttttca agaatggttt caaacggaaa
      121 accgagtatc gcaacttttt ttgtgtcaat tgtgatcact cccgctccta aagtttcgtc
      181 gttatcataa aaaagaaaac tctctccgtt tgaattaaat ggctcaatga catcaggaaa
      241 attgggctga tagatatatg ttccgtcatc aaaaagtcct gaaattgttg agaagtcgcc
      301 agttcctttg aaagtgtaga gatttgaaga gtcatttata tagtcagtat taagaacatc
      361 attaaagaaa gtaatatcag cagaatttcc tttttcaaca aggtcccatc cgatctcgga
      421 accgcttaca aaaaagctgc ctccatcaga aatatattga gcgatataac cctgctcgga
      481 aatggaaaaa gttgtgtcat ttgaggactg ttctccggca aaccagataa ctgcatcata
      541 ctttgaaaga tcaatggaac cgatgtttgt cttctgagtg aaatctgctg aaaaaccaag
      601 ctcttcaaac acttcaagat agtgaattac ataactataa ctgttaatga attcaagcat
      661 atatctattg cttccatttg tgccgactgc aaatctcgga gtgttctggt caaccctttc
      721 aaatccgtca acgatcagga tctgtgctcc ggttttttgg ggaactgcac ctgcgacaac
      781 gcttggaaat gaaacaccac cgtcgttata tgcaattatc ctgaaataaa gaggttgtcc
      841 cggctgcgga tcttcaactg taaattgagt aacatttccg acatctgttc cggtatcaaa
      901 actctttccg tcagtactca tctgaacaag atatccatct gcaggatgac cgagataaaa
      961 attctcatca ctttccggct catcccagaa cagagtgact gatccgtcat cattagtttc
     1021 agttctcaga ttgcgtggat gggagggaag atagaccgga tcgtagtcat cacggtctgc
     1081 aaaatatttt actatcgcct gataagccgc tcttgccgca atatttctga atttcgggtt
     1141 tttaagcatt gccgcatcaa gctcattatc atgaaatgca agttccacaa gacatgcagg
     1201 catttcattg ttataagtcg gattcaattc cccgaaataa gcactgtaca tcccgttacc
     1261 gatctgtttc cagcttgaat caaaaagtga tttcaccgca ttaaaaatcc ggttgtgaac
     1321 aagttcccca agctcgacgc ttcccgcctg tgcctcagtt gtaacataag ttgaacctgg
     1381 ttcatcactg ctgtagatat aagttgaaat gcctttggat gaaccgttga aggcatttga
     1441 gtgccatgaa atatatatgg aatcatcaac tcccgcttcg ttttcccatg cagtccatct
     1501 gcttcttgct gttacatcgg ctgaccggtc gttaactgat gactgataaa ctgttgacgg
     1561 tgctccgaga aactgggcat gaaccttggc cgcctcttcc catctcggtt ttccggatgt
     1621 tgaagttcct ctctttaaaa tagcattgcc tccaccgatc cggactgcat cggcaattat
     1681 tattgttccg ctttccgcat catcgttttt attaagcgta accgacccgc cggttccttt
     1741 tttgaaatag aactgaccga gatcgaccca ggtgcttccg tggtgtttct ggcttactgt
     1801 gacttcagtt tcacctccgg catgattgac tgtataaacc gcatcaggag ctctgtcggt
     1861 gaatgtttta tagctgacat agacatgata atttccatct tcagggatgt tcggtgtcca
     1921 ttttgcccag gcacctgttc ctgccgttgc atatctgtat tttccgtttt ccatgacatt
     1981 gctttcaatt tcgatcggga aagctgttct gccatatcct tttccgcttg ttgtttcagt
     2041 ccaggagccc ccttcttcat aagttcccga ctcaggaaaa gatgatccgt ctgcatcgtc
     2101 aactatcacc atggattcgt ttctgtccgt ttctcttgcg gaaaagacaa gtgcaccggc
     2161 attctgaaga tacggaacaa ggaaataact cacaatttct gcatttgaat catcttccct
     2221 catgccgaga ttaattcccc gctgagtgtt ccatgttccg ttgtcggtgt cgtataccca
     2281 gccgtgaccg gggcttgtga aaattgtttt tccggtaagt ccaccttcga tatttccata
     2341 tttcttttca ggaacaagtg attttgtctg tggagcggat tcgccttctt tttttggtac
     2401 tggcggaaga acaggcagga attttaagat atgctgataa gcttccagat tattcgttct
     2461 gacaaaaaaa gctatccctt taaaatcaat tttcttattt atcagaaagt taagataaaa
     2521 cctgttaagt gcttcgagat ttaagtcgtc aagcttctca atccatgact tatcaacggt
     2581 catatagatc gaaatgtatc tgctttcagt atcaacaacc gccttatcaa gagcaatttt
     2641 atttgagaag ccggagtcaa acagattaag aggaatatca aacttcgccg catttattgt
     2701 aagcataaaa aaaacagaaa ataataagcc gaataatttt ttcatatttt gccccgtaaa
     2761 aaagtccggt gcttattcta cttaatttta gggaaattga caataaaatt caaagtgtta
     2821 tattacaatc ttgaagtttt aatgtttttg cggggaaaac atggcagaat caactaaaaa
     2881 gccgctttac aaaacgattg cacaagattt gaaaagaagg atagatgagg gagaatttga
     2941 taaagagggc cggcttcctg cgataagaga tcttgctaaa gaatatttaa caactgctgt
     3001 aactatgact aatgcgatca gagaacttga tgaagagggg cttttgaaaa aagtgctcgg
     3061 cagtggaatt tatcttaaaa aacgggcagt tgatcctttt ttcccagttg acgatctaaa
     3121 gaaaatattc atttcggttc ttgaaaatga aggggcagaa gcttttgaat atggtgacag
     3181 ttcaggtttt tttggactta aagaagtggt aaaagaaaga gctctcaaaa aactgaataa
     3241 tatgaaagat cgtgagatac ttataacaag cggttcccag caggcgcttc atcttatttt
     3301 ttcttcaatg ctcaaagtgg gtgactgggt tcttgtcgaa gagccgacat accctgccgc
     3361 aataaggata ttgaaagaaa aaggggctca gatagagacc gttaaaatga aagacggagg
     3421 tcctgacata aaggaaataa caagaatatt tgattcccgt cctttaaaaa tggcctatct
     3481 catgccgaga ttccacactc ctaccggttt cagttatgat gaaaaagtta aaaagaccgt
     3541 ttctgatctt tcaaaaaaac acggcgttta catcgttgaa gacaattcat acggagagct
     3601 cgacttcgaa acgcacaaag aagttccgct cacagttgaa gccgtgcttc acatttattt
     3661 aagcagtttt tcaaagacat ttttaagcgg agctaggatt ggaacatgca tctgcggaaa
     3721 tgaagtttat gaaaagctca caaaggcaaa actcgatcac gacctttcaa gtcctctctt
     3781 tttccagatg gtgcttgcaa aatatatcat gagcggtcac tatgacaccc ggcttaaaga
     3841 actcaacaag ctctcaaaaa ataaatttga tgaaataaga caggggatga aagtcagttc
     3901 ttaaaattct ttgcgacatc ggcaatggtg aatccgtcga gttccttgat tatggtttcc
     3961 ttaactttga cagaaagtcg gcggaaaggg cacgattcgt cactgcatct gggtctgtcg
     4021 aaaaggcact gactgtgagg aaaattccct tcaagaagga ggatcagatc aagcattttt
     4081 attttttcag gtttcttttt gagaacatag cccccttttg ctcctcttga agagtcaaga
     4141 agaccatttt taacaatagg ttgaagaact tttgcaagat agctttctga aactgcaagc
     4201 tctttggcaa tgaatttaac agacacggca tccatttcag gatgtgccgc aataagtgca
     4261 agcgaatgta tcacgacatt ggtcttttct gtaatgttca gatttttctg cattattttc
     4321 ccttcctcac caaaaaatta tcttccttcc atcatagcgg cgacatcact atccgcatca
     4381 gttatacctt taatatcaaa tttttcaaca agaacttttg caacacctgc tgaaagaaat
     4441 ccgggaagtg tcggtccaag ccgtattccc tttactccaa gatagagcag cgcaaggaga
     4501 acaagaaccg ctttctgctc ataccatgca atgtcaaatg aaaggggaag tttgttgatg
     4561 tcgtcaagct caaatgcttc tgcaagtttt atcgcaatta ctgcaagaga atacgaatcg
     4621 ttgcactgtc ctgcatcaag aactctcgga attccgccga tgtcaccaag aggaagtttg
     4681 ttatatctgt attttgcgca tcctgctgta agaatgactg tgtctttcgg cagtttttca
     4741 gcaacttcgg tgtagtaact tctggtctta tgtcttccgt cgcagccggc catgacaata
     4801 aatctcttta ttgcaccgct tttaacggca tcgatcactt tgtcggcaag tgcgagagtc
     4861 tgtttgtgcc cgaatccgcc aacgatcttc cctttttcaa gttcaacagg tggctgacat
     4921 ttttttgcca tttcaattat ttctgaaaaa tctttgtgtc cgccctcagg tctgtcagca
     4981 atatatttca gtccttcaaa acccactgca ccggtagtga agattctttc cttataatca
     5041 agttccggcg gaacaagaca gttagtcgtg aaaagaacgg gaccgttgaa ttttttgatc
     5101 tcttctttct gaagccacca tgcgttgccg taatttccaa cgaggttgtc atatttctta
     5161 aacgccggtc tgtaatgagc gggaagcatt tcgctgtgag tataaacatc aacgccagtc
     5221 ccttttgtct gctcaagaag ctcctcgaga tcaacaaaat cgtgccctga aatgaggata
     5281 ccgggattgt tcctgactcc gagatttact tcagttattt cctgctcacc gtaatgggca
     5341 ttcgcctcgt caagatattt cataactgtg acaccccatt ctcccgtttt catggtaaga
     5401 gcgacaagtt catcggcagt tatttcgttt cctgcgatct ggtcaagtgc atacataaga
     5461 tattcaaaaa tttcccgacc ctgttttttg atctgataac cgtgctcaag ataagctgaa
     5521 attcctttca gaccataaag aacaagcgat ttaagtgaac ggatatcctc attttcaatt
     5581 tcaagccatc cgcctgcgcc aagagcagct tttgcataga tatcatcatc atttcccgga
     5641 acccattctg cacactcagg aattgccgta acggtttctt ttccgttcat aaaagttctt
     5701 attttctcac ggagtttaac cgcttcataa atatctgcaa tgaagtcctg cttattgaaa
     5761 tcggcatttg tgatggtttt aaaaagccct ttatcaacaa agaacgcagt tttttcatca
     5821 taccagccgt gcttttttgc tctcactgcc cagaaagaaa ttcctttcaa agaccagatg
     5881 aaaagatcct gaagatttgc agtttcagca ctttttccgc acacaccctg ttttgcactg
     5941 catcctctgt tcattgcagt ttcctgacac tgatgacaga acattttgaa ttcgttcatg
     6001 gtttcctcca aattaattaa aaaacagaca attgcgactc cattatccga attaaaagcc
     6061 ggtttgtcaa gtatatttca aacaattttt tcttaaattt ccaagtcttt gaaatcacga
     6121 cctgttgaaa gaataaattc aatataaaaa gagcagagtt ccgcctgttt tatgttcaga
     6181 tcgttttctg cttttactaa agaagtctgt gcctgaagaa gctcagttgt tgtcgagttg
     6241 cctgctttga aaagctcttc ttcaattttg aggttttctt ttgcatattc gacctgaact
     6301 tctccggttt ctatttctgt aagcgccttt ttaagcgaat tctgaagctg tctgatctca
     6361 acggtctttc ctgatttcag aagtgccaga tttaactgtg ttttggctgt ctgtgtctgg
     6421 gccgctttta ttttgtaaaa tccacttccc cactcccata tattccatga aaaaagtatt
     6481 ccggcataga attgattctg cggctgcata tctccgaatc cttcctgaaa atcatagttt
     6541 aagacaagat ttagttcagg aagaaatgat gataccgcgg catgtctgtt agactttaat
     6601 gcattaattc tttcttctgt gctttttatt tcaggaatgt tgttaagaaa atctacagtg
     6661 ttttctgcgt tatcattttc ggttatcttt tgtgtaaaag ttttaaaata ttctccgttc
     6721 cataccggct caagtggtga gtcaaggtcc ctgttcatga tcatgtttaa aataagtttt
     6781 gaattcataa ctgcaattga ggcggagatt tttccttttt caaaatctga ccgctgcatt
     6841 ttcagcttaa ggagtgcgtt cggctcaaca cgcccttcat caatgagtgc ttcaatcaca
     6901 ggatatgttt tttctatctg gtcaattgcc gcatcaaaac ttttttcgtt ttcaattgct
     6961 gaaagcattc tgaaataaac ttttgcaata tctttctgaa gctctctttt tattttcaat
     7021 gtgtcgtatt ctgccgcttt tttcattttt aacgaagcat tatgcagatt atatataccg
     7081 taaagagaca gaaccggctg aacaattgtc gccgatattt tataagcatg ttggtcatga
     7141 actgtaatgc ctgagctgtc actttcattc actgcattct gaattctctg tctgctgtca
     7201 ggtgaaagag tagggacaat ttcctgaatt acagcatcaa gaacgggcag atccattttt
     7261 gttttgttct cttcatccca gagcagagca ctggcagaca cttttaatac aggaagatat
     7321 tttccccgca cactttttga aatatatgac tgatattctt cttctgattt ttctgactgc
     7381 aattgaagat tctgttctaa agctaccttg atcacttcgc cgatggtcat tgattcagca
     7441 aaagcagtaa aatgatacag cacaggaata attaataaaa ttgataactt aattagtttt
     7501 ttcatttttt ggctccaatt tttgataact tttcactcat agaatcaaaa acatcataaa
     7561 agaccggaac gactatcatg gtaagaaatg ttgcggcagt cattccaccg ataattgccg
     7621 ttgccagcgg tgaaaatctt tcagatccga gcgcaagttc caaagaaagc ggtatcattc
     7681 cgacaacagt tgaaaacgat gtcatcataa taggtctgaa cctcaaagtt acagcgttga
     7741 tcaaagcttt cttccttttc accccccttt ctctttcctg gtttatgaac tctataagaa
     7801 taattgcgtt gttcacgaca attccgacaa gcagtatcag tcccaccatg accggcattg
     7861 aaatatattt acctgcaatg aaaagtgcca ggaaaacacc aatcatgctt aaggggatgc
     7921 ttatcataat ggtaacgggc tgaataaaag atctaagctg agcaacaaga agaagataaa
     7981 ccgcgataat ggcaataaga aacgctccga taagttcttt tttggattct ccaagatcac
     8041 ttttttctcc ggtaatagta attccgtatc cctgtggaag caaaaggtct gataatgatt
     8101 tttcaacatc atttattacg aaactcagtg ctcttccttc agaaaatgcc gaaacttcaa
     8161 gtgttgaaat aaggttttct cttgtaaaaa ctccctgtcc ctttctttct tcgatcttag
     8221 caacggctct taaaggtata atgtgaagag ttccaggggc aaaaagcgga aaccccagaa
     8281 tatcatcagg ttctgaagtg tctgttctgc tgtatctgat tagaacaggg tcaggatttc
     8341 ctgaatatcc gaaaaattca cctgctgaaa taccgtttga ccccatattc atagtttgtg
     8401 caacagttgc cggagaaatt ccaagttcca ccgccaactg atcatcaact ttgacagcaa
     8461 cgcttttatg atcaaaatcc cagcttctca ccggctctat cacattgggc acttgagaga
     8521 gtttcttaac aacctcgttc ccgatacggt caaggacaac cggctcattt cctgttatta
     8581 cagccacgat gggagctgat gttgtcgctt ttgcggtgtt tccgatctct ctgactgttg
     8641 atgcggcaat gccggggatt tttcttaatt tatttctgac tactgattgt atttcccaaa
     8701 tgctcctgtc tctttcattt ctggggctta aggttacggt tatacttccc tgtgtcggac
     8761 cctgaacccc gaatgaagac attgatttca ttccctgctc aaaaccggac tgtgactgaa
     8821 ttttctcaac ttccggcatt tccgttagaa aagactctat ctgtcctatt gctttagcgg
     8881 tttcttcaag agatgaccct gaaggcgtct gaagcgaaac ggaaaaactt cctccatcca
     8941 ttttcggcag ggtttccatt cccagactct gcaagccgat gatagaaaca acaaaaagaa
     9001 ttaaaacggc aaaaagcgtt actgttcggt gattcaaagc tttttcgagc acttttgtat
     9061 agaagttcct cagcatatcc attaaaaact ggaaaggctt tgccactttt tcaccgatcg
     9121 tatcaatttt gcttttgccg gcagtatata gtgttaaaac aggaaccagc acgaatgcga
     9181 ctgcaattga agatgagaag gcaaagataa gagtcagagc aagaggcccg aaagtcttgc
     9241 tcgtgaaacc ctcaagaaaa agaaggggaa ttagaacaga aagagttgtg gcaacacccg
     9301 ccaaaacagc cagtcccact tctgaggttc cgttaacaac cgccttttcc ggggattcac
     9361 cttcttccat cattcttctt gtaatgtttt caagaattac aattgacgaa tccacgacca
     9421 ttcccactgc gagaatgatg gctgaaaggg tcaccatgtt gaactcaaca ttaaaaagtt
     9481 tcattaaaac aaaagtaagc ccgaatgaaa gaggcataga aacaattgta acaagtgaac
     9541 ttcttattct gccaaggaaa agaaaaataa tgattgaagc gaataacagg gcctgccaga
     9601 cattcgaaag cagattgctg accgatattt cagtaaaagt tgcactttcc tcaccggtaa
     9661 tgaagctgta tgaactgtat tgagagtcaa gttcttttgc tttctcaaga gattttttca
     9721 caacttcaac ggtatttgca tcataagtct taaatatctg gatagcgatc gcatttctgc
     9781 cgtcaattga aaaacgggca tcatcatcaa gtgacccttt ttttatgctg gaaatttcac
     9841 caagtagaag tctgttaccg tttggaagag atatcggaat gtttctgatg tcttctatat
     9901 tttccgccct gctttcaaga cggaacattg tctgagtagt ttctgttcta atctgtccgg
     9961 cgggcattga atagttgaat tctctgactg cattgacaac tttctgaaac ggaattctgt
    10021 atgcctcgac atctcttctg ttaagttcga taattaaagc aggtttgttc ccgccgaaaa
    10081 catcaacagc tgcaacacct tgaattcttt gaagagttgg ggcaaaaaca tcttctgaca
    10141 tctttcgtgc cttggtaaga tcatcggaaa ttattcctat agtaacgaca ggtttgtcag
    10201 aggttgagaa ctttaaaacc tgagggtcgg taatgccggc aggaagagtg ttttttattc
    10261 ttgagattga attctgaata tcaactgctg ccagatcgac atccctttca taatcgaact
    10321 cgaccgaaat gatggaaaga ttgtcctgag atgttgcttt tgttttgaca actccgtcaa
    10381 gtgatgcaaa ctcctcttca agtttctggc tcagtgtttc atttacatct tttgctccgg
    10441 ctccggggta tgctgttatg acattgatga gaggcggagc tgtgtcaggg aaaagctgca
    10501 tcggaattga aaagtaggcc tgaatcccga aaataacagc tgctatgaca agcgcccaca
    10561 ccacatattt attttttatg agcccttctg taaatctcat agcttttccc cttttagaaa
    10621 tacagggtaa actgtcatgc cgctgcttaa tgcttcaaga tttccaacaa caactttact
    10681 gttttcgggg atgtcgccat caattgtcac ccatcctttt tcagaaatcc ccaagacgat
    10741 ttttcttttt tcgatctttt catcttcaaa aaggaagata tattcgcttt ccccatcttt
    10801 cagaactgaa ttcgaaggca ctgaaaaaga gtttttcttt tctgaaacga taaaactgac
    10861 atttattgaa tgtccatgag aaaaattttg caaaaggtct ttttttaacg gaatgtgagt
    10921 ttcatacatt ctgccggggc ctgctgaaat aggcgaaaca aaagatacag cagattcagc
    10981 actttgatct tcattatttt cgagaatcac tttcaacccc ttttttatcc cttttgagat
    11041 atctttttca tgaacgaaaa ctttaactat gtaggagtca tcgccaaaaa ggagaatcgg
    11101 aaaccccggc atgatatttt ctcccggatt gctgaaccac tttaaaacag tgccgctaaa
    11161 aggtgcactc tcaactgttt ttgagctcat tatttcaagt tcatcaagtg acgaagttgc
    11221 tgaattaatt gagagtttgc tgttttcgca attcttctta cttgtttcag cccgtattga
    11281 aggaactgca ttcttttcaa aaagccgctc atcagcttct gcctgatcgc agataaactc
    11341 tgattctttt tgtgcttttg caatttcctc tttcatcttt gatattctcg ctcctgtttc
    11401 aggggcactt atttttgaaa gaacggcacc ttttttcaca ctcattccct ctttaaaagg
    11461 aagtgccaag atatttccgt ttattcttgc aaggatcctg atttcatttt cagagtgaac
    11521 agagccgata tacctgattt ttttagatgc atcacctttg cggacggcaa ttattttaac
    11581 agccaccggg gtttctctta cgatttcagt tttttctgtg ctgcaggata aaaaagcaag
    11641 ggtagataaa aggaaaagag aaattaatat cggtattgtt ttgattttcc cgtttttcat
    11701 gactgtctcc ttattaattt ttcccaggtt tcccacattc cggcagatat tttctttaac
    11761 tccctttctt tcattaatga tgtgtaaagg tggctgttat aaatgcttaa aattgatccg
    11821 agaattagaa ctgtaagatt gtcaggatca agtccgtctt ttaaaagacc ggcgttaaac
    11881 gcatcattaa gtgttgaatg gacaaacttc agcgattttt cacgcatttc tttaattatt
    11941 gcaacacctt cgctaccggc aatttttgaa aactgatctg agaacataac attcattgcg
    12001 tttcgatttt taaaaagaac tgaaattctt tttttaaaga actgtccgag gcgttcaacg
    12061 ggatctccgc taaaatctgc cgatccttca aagaacttat gttcaattga cttgatgatg
    12121 tatagaacaa tatcattttt agt
//
LOCUS       DAOOYB010000074        12067 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_5525_length_12067_cov_11.505828, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000074 DAOOYB010000000
VERSION     DAOOYB010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12067
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5525_length_12067_cov_11.505828"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="PL056_05905"
     CDS             complement(<1..237)
                     /locus_tag="PL056_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46584.1"
                     /translation="MKDNFKHYQVFVFAALFLIIPELMFGAADTSGVKSKFTEMADTV
                     FDVARYIGLYGGLGGAIISLLFRYINKDQNIWSDA"
     gene            complement(396..764)
                     /locus_tag="PL056_05910"
     CDS             complement(396..764)
                     /locus_tag="PL056_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46585.1"
                     /translation="MDSYTIGKILGWLSVIALAVLLLNFVTVKIIKKPKFLRKITSAL
                     HTPAAVTLVVAAVIHSYLIWGNLRIHTGHILFFGILLTAAASILGKKYKFKKWLPLHI
                     YLAFATLALFLVHYFFPYLV"
     gene            900..1361
                     /locus_tag="PL056_05915"
     CDS             900..1361
                     /locus_tag="PL056_05915"
                     /inference="COORDINATES: protein motif:HMM:NF016102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HPM46586.1"
                     /translation="MFFKLFILFTIIPVLELWLLIEIGGVIGFFPTVMVVIITGISGA
                     WLAKMEGISVLTKIQRSMSQGVIPKTELVNGLLVFVGGAMLLTPGFITDITGLLMIFP
                     PTRAIFSTVLISYFERKIKDGTVHFYHSGQTENKTRIDDESVIDADFEEKK"
     gene            1380..1745
                     /locus_tag="PL056_05920"
     CDS             1380..1745
                     /locus_tag="PL056_05920"
                     /inference="COORDINATES: protein motif:HMM:TIGR04134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM-associated putative lipoprotein"
                     /protein_id="HPM46587.1"
                     /translation="MLRFIKKSLLWIVSGTVSVILTACYGVASMAECFGKKITVTDSE
                     SNPIKGLEITVSDAGGKQDLVLTDEAGVAEINFCTNYGKVTIEISDVDGAENGEFKEK
                     TVVFLTDDNPETVEMESVE"
     gene            1742..2800
                     /locus_tag="PL056_05925"
     CDS             1742..2800
                     /locus_tag="PL056_05925"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPM46588.1"
                     /translation="MILKIREKAWELYRNIEQREHNLQYLFLEITKKCNLNCLHCGSD
                     CKAEYSTAELTTESWLKIIDYVKAHFGEQVLFVISGGEPLVHPDIFKIGQHITDTGMK
                     WGMVTNGIKLDGKCMKNLVDSGIYSITISVDGMEKNHNFLRNHKSAYKKVEAALKLIG
                     NAPISMKDAVTCVYPANLNELDKIAELLIKNNIPAWRLFRIFPNGRAEKNIELHLTKE
                     NTKEMLNWIAEKRPFYKKQDLNVSASCEGFLPFKQDVTVRDYPFFCRAGINIAGILSD
                     GTITGCTNNHEGFAQGNILKDDLATVWERKFEDFRKRKWIENTDCASCRYFRKCRGGS
                     IHLWKKGEKSPRFCYVEK"
     gene            complement(2802..3149)
                     /locus_tag="PL056_05930"
     CDS             complement(2802..3149)
                     /locus_tag="PL056_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013010576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HPM46589.1"
                     /translation="MERFYPFFLLIGSNLFMTYAWYGHLADFKNRSLFVVILISWGVA
                     FFEYCLQVPANRIGFQFYSLGQLKVMQEIITMGVFALFSVLYMHEKLTLDFLYASICL
                     AGAAFFMFRNVAI"
     gene            complement(3164..4600)
                     /gene="cysS"
                     /locus_tag="PL056_05935"
     CDS             complement(3164..4600)
                     /gene="cysS"
                     /locus_tag="PL056_05935"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015404469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HPM46590.1"
                     /translation="MLKIYNTLTEKKEEFVPIHEGKVGIYACGMTVYDKPHIGHARKE
                     VAIDLIVNYLKYCGYEVNYVRNFTDVDDKIIARANERKIGFTELSEENIDAFYQAVDA
                     LGLQRPDVEPKATEHIPEIIDMIEKLIKNGYAYPVEDGSVYFAVEKFKGYGKLSNRKL
                     DELIAGTRFEPEAGKRNPLDFALWKASKPEEPKWKSPWGEGRPGWHIECSAMSTKYLG
                     DTFDIHAGGRDLIFPHHENEIAQAECATGKPFVKYWVHNGFLTVEGEKMSKSLHNFIT
                     VEDALVQYHPEVLRYFMLSTQYRTPIDFSMSNLKETEKRIYYFYNTLKALNQFAIKSE
                     GTAQKNDKAIEFINEFKESMDDDFNSARVVAAMMSLFKHLNEVLVSKKTRPSSAECAG
                     FAEVIKEAGKVLGVLLRDPDEIINEIKEIQLRRYGITKAEIEDIIEKRKQFRTEKNFE
                     EADKMRDVLLSKGISIQDTPLGTDWSFN"
     gene            4763..6724
                     /locus_tag="PL056_05940"
     CDS             4763..6724
                     /locus_tag="PL056_05940"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HPM46591.1"
                     /translation="MIDLEKRHDILREWKKFMNSMPVDPAVIHPYLIASWERSRNYGI
                     TQEMKDYPRISSGELVNRIHENRKMLSIAVPYMKKIIGFMNDPESIILITDKNGIVLE
                     LFGSDEMKKIDSSPKLGSRLLEQNIGTNGVGTAIVENRTLDIWAGEHFLEDLHFWYSS
                     ADTIHDSQGKIIGCLCLVCRWDLVNTHTLGMVGGVSGAIERQYQLEETLAGKILEIKK
                     QQAIVESINEGIIVIDAEERITGLNPQAKLMLDLDNRDYSGRSIYEIILSGVNFFKIM
                     TGGTNIYDREATLKLKSKLFHCTMSTSLIQSEGSVNGMVITIRESKALHQLAGKIAGS
                     RAYISFSDIIGNSSAIQEAIKVAKTAGKSKSNVLLLGESGTGKELFAQSIHNHSDRAN
                     SSFVAVNCGAIPRDLVESELFGYEGGAFTGSRKEGRPGKFELADEGTIFLDEIGDMPL
                     DAQVNLLRVLQNSEVVRVGGTYPKKINVRVIAATNHDLKKAVEHKTFRDDLYYRLNVL
                     SITIPPLRERVEDISSLANHFARKTAHNLNKTITEITPKAMFMLERYGWPGNIRELEN
                     VVERAVNIMESAVITPAELPDYIRNSSVEVSTLEPQSLKAREYEIIVSMLSETAGNLR
                     ETAKRLGIARGTLYKKITKYGIEAEKFRF"
     gene            complement(6753..7514)
                     /gene="panB"
                     /locus_tag="PL056_05945"
     CDS             complement(6753..7514)
                     /gene="panB"
                     /locus_tag="PL056_05945"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HPM46592.1"
                     /translation="MKKVTMPALVKKKGVAKISMVTCYDASFAKLVEKAEIDSILVGD
                     SLGMVIKGDTNTLEVDISEVEYHVKAVRKGAKTPFLIADMPFGSYQITVAKGLKNAVA
                     LMKAGAEAVKVEGGKEVSKLISTLTSYGIPVVGHVGLTPQYVNKFGGFGKRGKTPEER
                     KFIFDSALAVEKAGAEMIVLESIPEELANEITSALKIPTIGIGAGKNTDGQVLVIYDL
                     LGMDENFNPSFLKKQCNLDQIITNALKTYKLETEK"
     gene            complement(7511..8326)
                     /gene="rsmA"
                     /locus_tag="PL056_05950"
     CDS             complement(7511..8326)
                     /gene="rsmA"
                     /locus_tag="PL056_05950"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HPM46593.1"
                     /translation="MSLSEIKHYLSKTGSYAKKNFSQNFLFNDDTLYKIVKLVPDGKG
                     FYVEIGGGLGSLTKIAVDRKLLPLTVADLDDGMLTVLKERFGEVANIISQDGAKIDFS
                     QYFNGVKGFVFGNLPYQVSSPIVMNTVFNSTNLDGAVFLLQKEVAEKFCAKPGTRNFG
                     PVAALIQLTGCAEYIYTLAPEEFYPAPKVYSSVLKISFKDHNYNAMELKIFADFVRVL
                     FSNRRKTLSNVFKINNLDMKILEILNISATKRAEELDWDSILKIGDHLSGGRK"
     gene            complement(8323..9333)
                     /gene="tsaD"
                     /locus_tag="PL056_05955"
     CDS             complement(8323..9333)
                     /gene="tsaD"
                     /locus_tag="PL056_05955"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HPM46594.1"
                     /translation="MTEYILGIESSCDDTSVAVVDSNYAVLSLKTVSQTSIHKLFGGV
                     IPEIASRNHIAWIIPALDAVIEESGIKPEDLSAVAVTNYPGLIGSLLIGVSVAKGLSI
                     GWNKPLIAVNHLDAHIASAFIGKKNNPVFPYLALVVSGGHTSLIEVTENGKSVLGETM
                     DDAAGEAFDKVAKMAHLGYPGGPVIDKMAQNGEAAKYKLPYLLKNNNKYIDEIIFSFS
                     GIKSAVKRVLDEGNVDMDSLMASFQERIIELIERKMKLAISRKEYKSIVISGGVSANS
                     GVRKMASSLAHKNGLELFMPELKYCQDNGAMVAAAAFDDFFKGNFSSLDFDVAPTVRP
                     RI"
     gene            complement(9336..10181)
                     /gene="nfo"
                     /locus_tag="PL056_05960"
     CDS             complement(9336..10181)
                     /gene="nfo"
                     /locus_tag="PL056_05960"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HPM46595.1"
                     /translation="MKLIGAHVSASGGVENAPVNAASIGANAFALFTKNQKQWFASPL
                     TKSSIDEFIENCEKYGIRRTAVLAHDSYLINLGHPQQEGLEKSRKAFIDEMKRCELLR
                     LDKLNFHPGSHLREISETECLARIAESINIALGKTRGVSAIIENTAGQGSNVGYKFDH
                     LAEIIDKVEDKTRVGICIDTCHAFTAGYDIKTEEACVRTFGELDMLVGLEYLKGMHLN
                     DSKKPHESRVDRHETLGNGFIDIVCFKWLMNQSFTDDIPLILETPEPEKWKDEITLLR
                     SFVRN"
     gene            complement(10209..10925)
                     /locus_tag="PL056_05965"
     CDS             complement(10209..10925)
                     /locus_tag="PL056_05965"
                     /inference="COORDINATES: protein motif:HMM:TIGR02495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HPM46596.1"
                     /translation="MLPITGHQQTSLIDFPGKIATVFFFAGCDLRCPYCHNSSLFEVN
                     KEAILSKEYVLSEIEKRSKFIDGVSFSGGEPTLYEEFLGFLKEIKEKFKLEIKIDSNG
                     LRPEFIEKALPYLSHIAIDIKTTPDLYSKLGCMLTKDEVKQKLLKTKEILENSKGVKI
                     EYRTTMYPPIVESYERVYKMFEFIPENAEYYLQRFISDNACSAEAKNTKSYTVEQLER
                     MALEIRRNTRKEKVFVRTYA"
     gene            11077..11928
                     /locus_tag="PL056_05970"
     CDS             11077..11928
                     /locus_tag="PL056_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46597.1"
                     /translation="MRNLVLKMVTEGVLTSENANKMLAMKKGVDPVWRALSKGLIDEK
                     KLARFYEKEGYPVIYDEGRGPMSDDFFTRFFTPDVIARHLAVPISFKKESRDIIVGFI
                     NAALIPQIKENILKIFPEFNTTFFHIPYSIFKKIAAKHFLFDIDKYTSVMIQMEVSGE
                     MHSDKVVSVSSVRKKLREAAEQVYMLEIDAQGSVVFKEESMTSRFPLKSLPIFKEGFE
                     RGYMEIIPGDNLNSLSHTEKILLFTNVGIKNDDKIMILAADEEKKTFVLLLNPKVPKD
                     QIDFLLR"
BASE COUNT         3618 a         2403 c         2474 g         3572 t
ORIGIN      
        1 agcatcgctc cagatgtttt gatccttgtt tatatatctg aaaagaagag atatgatcgc
       61 tccgccaaga ccgccgtaaa gaccgatata tcttgccaca tcaaacacag tgtcagccat
      121 ttcagtgaac tttgatttca ctcccgaagt gtcggccgca ccgaacataa gttccggaat
      181 tattaaaaat aatgccgcaa aaacaaaaac ctgataatgc ttaaaattgt ctttcatcag
      241 ttcctccaag tttttcaaat gctttcaaat atcgaagcag gcaactttat tatctaacat
      301 cgagtagagt ataaatggag ctatcttaaa tgcaagaaaa atttaacgac tgtttaaatt
      361 agtatgttgt tggagcgaat gtttgcagtg actgctcaca ccagataagg gaaaaaataa
      421 tggacaagga aaagggcgag tgtcgcaaaa gcgagataaa tatggagcgg gagccatttc
      481 ttgaatttgt attttttgcc gagtattgat gcggcggctg tcagaagaat tccgaaaaag
      541 agaatgtgtc cggtgtgaat gcggagattt ccccagatga gatacgaatg aattactgcc
      601 gcaacaacaa gtgtaactgc cgcgggagta tggagagccg aagtgatctt tcttaaaaac
      661 ttcggttttt taattatctt aactgtcaca aagtttaaaa gcagtacagc aagtgcaatg
      721 actgaaagcc agccgagtat ttttccgatc gtgtaactgt ccattttttt cctccgtaat
      781 gatccttagt ttttaaagta tataaaattc ctgatttttt ggaatatttt caaatgatct
      841 ctttttttga cacttttatc gctaactgat attgttttgc agaaaaaacg gaggtattaa
      901 tgttttttaa gcttttcatc cttttcacaa ttattcctgt tcttgaactg tggcttttga
      961 ttgagatcgg cggagttatc ggcttttttc cgactgtcat ggtcgttata atcacaggta
     1021 tttcaggtgc gtggcttgca aaaatggaag ggatctcggt tctgacaaag attcaaagat
     1081 caatgtcgca aggagtgatt cccaaaactg aacttgtgaa cggacttctt gtgtttgtcg
     1141 gcggagcaat gctgctgact cccggattta tcactgacat cacgggactt ctgatgatat
     1201 ttccaccgac aagagcgatt ttttcaaccg ttctcatttc atattttgaa agaaaaatta
     1261 aagacggaac tgtccatttc taccattcag gacaaactga aaacaaaacc cgaatcgatg
     1321 atgaaagtgt aatagatgcc gattttgaag agaagaaata actttttttg gagtgttaaa
     1381 tgctcagatt tattaaaaaa tcacttcttt ggatcgtgag cggaacagtt tcagtaatcc
     1441 tcaccgcatg ttacggtgtt gcttcaatgg cggaatgttt cggaaaaaag ataacagtca
     1501 ccgactctga aagcaacccg ataaaagggc ttgagatcac agtttctgat gctggcggca
     1561 agcaggacct tgttttaaca gatgaagccg gggttgctga aataaatttc tgtacaaatt
     1621 atggcaaagt tacaattgaa atcagtgatg ttgacggcgc tgaaaacgga gagtttaaag
     1681 aaaaaacggt tgttttccta accgatgata atccggaaac tgtggaaatg gaatctgtcg
     1741 aatgatcctc aaaatcaggg aaaaggcgtg ggagctttat cggaatattg aacagcggga
     1801 acacaatctg caatatcttt ttcttgagat cacaaaaaaa tgcaatttga actgtcttca
     1861 ctgcggctca gactgcaaag ctgaatattc cacggcagaa cttacaactg aaagctggct
     1921 gaaaataatt gattatgtca aggcgcattt cggtgaacag gtgctttttg tcatttccgg
     1981 aggagaaccg ctggttcatc ctgatatttt taaaattgga caacatatta ctgataccgg
     2041 aatgaaatgg ggaatggtca caaacggcat caaacttgat ggaaaatgca tgaaaaacct
     2101 tgttgattca gggatttaca gcataacaat aagtgttgac ggaatggaga aaaaccataa
     2161 ttttttaagg aatcacaaaa gtgcatataa aaaggttgaa gcagctttga agcttattgg
     2221 aaatgcaccg atatcgatga aagatgctgt gacatgcgtc tatcctgcaa acctcaatga
     2281 acttgataag atagctgaat tgctgataaa aaataatatt ccggcatggc ggcttttcag
     2341 aatttttcca aatggaagag ctgaaaaaaa cattgaactt catctgacaa aggaaaacac
     2401 aaaggaaatg cttaactgga tagctgaaaa aagaccgttt tacaagaaac aggatctgaa
     2461 tgtttcagca agctgtgaag gatttcttcc gtttaaacag gatgttacag tcagagacta
     2521 tccttttttc tgccgggcgg ggataaatat cgcaggaatc ttaagcgacg gaacgatcac
     2581 aggatgcacc aacaatcacg aaggttttgc acaggggaat attttgaaag atgatcttgc
     2641 aacagtctgg gaaagaaagt ttgaagattt cagaaaaaga aaatggattg aaaatacaga
     2701 ctgtgcttcc tgcagatatt tcagaaaatg ccgaggcggc tccatccacc tctggaaaaa
     2761 aggagaaaaa tcaccacgat tctgttatgt tgaaaaataa tctaaatagc tacattcctg
     2821 aacatgaaaa atgctgcgcc ggctaggcat attgaggcat acagaaagtc gagagtcagt
     2881 ttttcgtgca tgtaaagaac tgaaaacagt gcaaaaacgc ccattgtgat tatttcctgc
     2941 ataactttga gttgacccag cgaatagaac tggaaaccga tgcggtttgc cggaacctga
     3001 agacagtatt caaaaaatgc aaccccccag ctgataagta ttacaacaaa caaagacctg
     3061 ttcttgaaat cagccagatg gccataccag gcataagtca tgaaaagatt cgacccgata
     3121 aggagaaaga aaggataaaa ccgctccatt gcaacctcct gaattaattg aaactccagt
     3181 cagttcccaa aggtgtgtcc tgaatcgaga tcccttttga aagaagcaca tctctcattt
     3241 tatcggcttc ttcaaagttc ttttcagttc tgaactgctt ccttttttct attatatctt
     3301 caatttcagc tttcgtgatg ccgtatcttc taagctgtat ctcttttatt tcattgatta
     3361 tttcatcagg atccctcaga agaactccga gaacttttcc tgcctctttt attacttcag
     3421 caaacccggc acattcagca gaagagggcc ttgtcttttt tgaaaccagc acttcgttaa
     3481 gatgtttgaa cagcgacatc attgctgcga ccacccttgc actgttgaaa tcgtcatcca
     3541 ttgattcttt aaattcgtta atgaattcaa tggctttgtc attcttttgg gcagtgcctt
     3601 cagattttat ggcaaactgg tttaaagctt tcagagtgtt gtagaaataa tagatccttt
     3661 tttcagtttc tttgagattt gacattgaaa aatcaatggg tgtacggtac tgcgttgaaa
     3721 gcatgaaata gcggagaact tcaggatgat actgaacgag agcatcttca acagtgatga
     3781 aattatggag cgatttgctc attttttccc cttcgacagt tagaaatccg ttgtgaaccc
     3841 agtattttac aaatggcttg cctgttgcgc attccgcctg agctatctca ttttcatgat
     3901 gaggaaagat caggtctctt ccgccggcat gaatatcaaa agtgtcgccg agatattttg
     3961 tgctcattgc actgcattca atatgccagc ccggacgacc ttcaccccac ggacttttcc
     4021 atttcggttc ttccggtttt gaagctttcc agagtgcaaa atcgagcgga ttgcgttttc
     4081 ccgcttcggg ttcaaacctc gttcctgcaa tgagttcatc aagttttctg tttgaaagtt
     4141 ttccgtaacc tttgaatttt tcaacagcaa aatagacact gccgtcttca acaggatatg
     4201 cgtaaccgtt ttttatcagt ttttctatca tatctataat ttcaggaatg tgctctgtcg
     4261 ccttgggttc aacatcaggc ctttgaagtc cgagagcgtc aactgcttga taaaacgcat
     4321 caatattctc ttcgctgagt tcagtaaatc cgatctttct ttcattcgct ctggcaatta
     4381 ttttgtcatc aacatctgtg aagttcctga catagtttac ttcatatccg caatatttca
     4441 gataatttac aataagatca atagcgactt cctttcttgc gtgaccgatg tgcggcttgt
     4501 cataaacggt cattccgcag gcataaattc caaccttccc ttcatgaatc ggcacaaatt
     4561 cctctttctt ttcagtcagt gtgttgtaaa tcttaagcat ccggctctcc tcaaagatta
     4621 taaaaataag ctgaatagta tttatatctg agccagtcta taagtcaagg aacaacatgt
     4681 tattgctaat taatggaaag attttacttt tcatggagtt tttcataata tcttcagatt
     4741 cgtgttttat aatggtgtta agatgatcga tcttgaaaaa cggcatgaca ttttaaggga
     4801 atggaaaaag tttatgaatt ccatgcctgt tgatcctgcg gtcatacatc cttatcttat
     4861 cgcttcatgg gaaaggagca gaaattacgg aataacgcag gagatgaaag attatccgag
     4921 aataagctcc ggtgaacttg tcaacagaat acacgaaaac cggaaaatgc tttcaattgc
     4981 agtgccttac atgaaaaaga tcatagggtt tatgaatgac cctgaaagca taattctcat
     5041 cacagataag aacggaatag tccttgagct tttcggatca gatgaaatga aaaagataga
     5101 tagctccccc aagctcggaa gccgtcttct tgagcagaat atcggcacaa acggtgtcgg
     5161 aacggcaata gttgagaaca ggacgcttga catctgggca ggggaacatt ttcttgaaga
     5221 tctccatttc tggtattcaa gtgccgatac gatacatgac agtcaaggga aaatcatcgg
     5281 ctgtctctgt ctggtgtgcc ggtgggatct tgtaaatacc cacactctag gaatggtcgg
     5341 cggtgtttct ggagcgatag aaagacagta tcagcttgaa gaaactcttg ccggaaaaat
     5401 tcttgagatt aaaaaacagc aggcgatcgt tgaatctatc aatgaaggaa taatagtcat
     5461 cgacgctgaa gaaaggataa ccggacttaa cccgcaggca aaactgatgc ttgatcttga
     5521 caaccgtgat tattccggca ggtcgatata tgagataatt ctcagcggag tgaatttttt
     5581 taagatcatg accggaggaa caaatattta tgacagagaa gcgactttaa agctcaaaag
     5641 caagctgttc cactgcacaa tgtcaacttc gcttattcag agtgagggaa gtgtgaacgg
     5701 aatggtcata acgatcagag aatcaaaggc acttcaccag cttgcaggaa agatagcggg
     5761 ctcaagggca tacatttcat tttcagacat aattggtaat tcttcagcca ttcaggaagc
     5821 tataaaagtt gccaaaactg ccggaaaaag caagtcgaat gttcttcttc tcggtgaaag
     5881 cggaacagga aaagagcttt ttgcccagtc aattcacaat catagcgaca gagcaaacag
     5941 ttcttttgtt gccgtgaact gcggagcgat tcccagagat cttgttgaga gcgagctttt
     6001 cggatatgaa ggaggagctt tcaccggctc aagaaaagaa gggcgtcccg gaaagttcga
     6061 acttgccgat gaagggacaa tattccttga tgaaataggt gacatgcccc ttgatgctca
     6121 ggtaaatctt ctgagagttc tgcagaattc agaagttgtg agagtaggcg gaacatatcc
     6181 caaaaagata aatgtcaggg tcatagccgc tacaaaccat gaccttaaaa aagcggtgga
     6241 acataaaact ttcagggacg atctttatta ccggctcaat gtcctttcaa taaccattcc
     6301 cccattaaga gaaagggtgg aggacatctc aagtcttgcc aaccattttg caagaaagac
     6361 ggcacacaac ctgaataaaa ccattactga aataactccc aaagctatgt tcatgcttga
     6421 aagatacggg tggcctggaa atatcagaga gcttgaaaat gttgttgaaa gagctgtgaa
     6481 cataatggaa agtgctgtga taacacctgc ggaactgcct gattatatta gaaattcttc
     6541 tgttgaagtt tcaactttgg agccacagtc actcaaagca agggaatatg aaataatagt
     6601 ttcgatgctg tctgaaactg ccggcaatct ccgtgaaacg gctaaacggc tcgggatcgc
     6661 tcgcggcaca ctttataaaa agatcacaaa atacggaata gaagccgaaa aattcagatt
     6721 ttaaacaaaa atttctttaa aaggatttag tatcactttt ccgtttctaa tttataggtt
     6781 ttcagggcgt ttgtgatgat ttgatcaagg ttgcactgtt ttttgaggaa tgagggattg
     6841 aaattttcat ccattccgag aaggtcataa attacaagca cttgtccgtc tgtatttttt
     6901 cctgctccga tgccgatggt gggaattttc agtgccgagg ttatttcatt tgcgagttct
     6961 tcgggaattg actcaagaac tatcatttca gcacccgctt tttcaactgc aagggctgaa
     7021 tcaaatatga actttctttc ttcgggcgtt ttgccccgtt tcccgaagcc accgaattta
     7081 ttgacatatt gaggagtaag tcccacatgc ccgacaacag gaatgccgta agaagtaaga
     7141 gttgaaataa gttttgaaac ctctttcccc ccttcaactt ttaccgcttc cgcccctgct
     7201 ttcatgagcg caactgcgtt tttcagccct tttgcaacag tgatctgata acttccgaaa
     7261 ggcatatcgg caattaagaa cggggtttta gccccttttc ttacagcttt gacatgatat
     7321 tcaacctctg aaatgtcaac ttcaagagtg ttggtgtctc cttttatcac cattccgaga
     7381 ctgtcgccga caagaattga gtctatttca gctttttcta cgagtttggc aaaacttgcg
     7441 tcatagcatg ttaccatcga gatctttgcg acaccttttt tcttaactag tgccggcata
     7501 gtcacttttt tcattttctt cctcccgaca gatgatcccc gattttaagg attgaatccc
     7561 aatcaagctc ctctgccctt ttcgtagctg aaatgttaag tatttcaagt atcttcatgt
     7621 caagattgtt tattttgaaa acatttgaga gggttttcct tctgttggaa aaaagaactc
     7681 tcacaaaatc tgcaaatatt ttaagttcca ttgcattgta attatgatct ttgaaagaga
     7741 tcttcagaac tgaactgtaa actttcggag caggataaaa ctcttccgga gcaagtgtgt
     7801 aaatatattc tgcacaaccg gtaagctgaa tgagtgcggc aacaggaccg aaatttcttg
     7861 ttccgggctt tgcacagaat ttttcggcga cctctttctg gagaaggaat accgctccgt
     7921 caagatttgt gctgttaaag acagtgttca taactatagg agagcttacc tgatagggga
     7981 gattgccgaa aacaaaacct ttaactccat taaaatactg agaaaaatca atttttgcac
     8041 cgtcttgact tataatgttc gcaacttcac cgaaccgctc tttgagaaca gtaagcattc
     8101 cgtcgtcaag atctgcaaca gtcaaaggga gaagctttct atcaacagcg atctttgtca
     8161 gagagccgag tccgccgccg atctcaacat aaaaaccctt tccatcaggt acaagtttga
     8221 caattttata aagagtatca tcgttaaaaa gaaaattctg tgaaaagttt tttttggcgt
     8281 aactacctgt ttttgataag taatgtttta tttcagataa gctcatatgc ggggtctcac
     8341 agtgggtgcg acatcaaagt caagcgacga gaaattccct ttgaaaaaat cgtcaaatgc
     8401 cgccgcggca accattgcac cgttgtcctg acaatattta agctcaggca tgaaaagttc
     8461 aaggccgttt ttatgagcaa gagatgaagc catttttcta acccctgaat ttgcacttac
     8521 tccgcccgaa attacaatgg atttatattc ttttctactt attgcaagct tcatttttct
     8581 ttcgataagt tcgatgatcc gctcctgaaa agaggccatc agcgaatcca tatcgacatt
     8641 tccttcatcg agcacccttt ttacagcaga ttttattccg ctgaaggaaa atattatttc
     8701 gtctatatat ttgttattat tctttaaaag atacggtagt ttatattttg ccgcctctcc
     8761 gttttgagcc attttgtcta tcaccggtcc gcccggataa ccgagatgcg ccatctttgc
     8821 aactttgtca aatgcctctc ctgccgcatc atccattgtt tctccgagaa cacttttccc
     8881 gttttcagtc acttcgataa gagatgtgtg tccacctgaa accacgaggg caagataagg
     8941 gaatacagga ttattttttt tcccgataaa agcgctggct atgtgtgcat caaggtggtt
     9001 tactgcaatt aacggtttgt tccaaccgat cgaaagacct ttcgcaaccg aaactccgat
     9061 aagaagactg ccgatgagcc ccggatagtt tgtcactgca actgccgata aatcttcagg
     9121 ttttatcccg ctttcctcta tgactgcatc aagtgccgga atgatccagg caatgtgatt
     9181 tcttgacgcg atctcgggga tgacaccgcc gaaaagtttg tgaatgctgg tctgcgaaac
     9241 agtttttaaa gaaagaactg cataatttga atctacaacc gcaacagaag tgtcatcgca
     9301 gctgctttca attccaagaa tatattcagt cattgtcaat tccttacaaa acttcttaaa
     9361 agcgtgatct catctttcca tttttccggc tcgggagttt caagaataag tggtatgtcg
     9421 tctgtgaaac tctggttcat aagccatttg aagcacacaa tgtcgataaa gccgtttccg
     9481 agagtttcat gccggtcaac cctgctttca tgcggctttt ttgaatcgtt cagatgcatt
     9541 ccctttaaat attcaagacc gacaagcata tcaagttcac caaaagtcct tacacatgct
     9601 tcttcagttt ttatatcata tccggcagtg aaggcatgac atgtatcgat acagatgcct
     9661 actcttgttt tatcttcaac tttgtcaatg atttcagcga gatgatcaaa tttatatccg
     9721 acattcgaac cctgtcccgc agtgttttct ataattgcag aaacacccct tgtcttgccc
     9781 agagcgatgt ttatcgattc ggcaattctt gcaagacact cagtttcgct tatttcccgc
     9841 agatgactcc ccggatggaa gttcaatttg tcgagccgga gcagttcaca ccttttcatt
     9901 tcgtcaatga atgcttttct tgatttttca agtccttcct gctgaggatg tccaagattg
     9961 atcagatagc tgtcatgggc aagaacggcc gttctccgga taccgtattt ttcacagttc
    10021 tcaataaatt cgtcaatact gctctttgtc agaggtgaag caaaccactg tttctggttt
    10081 tttgtgaaca aagcgaaagc attcgccccg atggatgccg cattcaccgg agcgttctcg
    10141 actccgccgg aagctgaaac atgtgcacct ataagcttca ttcaccatac ctctataata
    10201 atatatcttt aagcgtaggt tcttacaaag actttttctt tcctggtatt ccttcttatt
    10261 tcaagagcca ttctttcaag ttgttccaca gtatatgatt ttgtgttttt agcttcagcg
    10321 gaacaagcgt tgtcgctaat gaatctttgg aggtagtact cagcattttc aggaatgaac
    10381 tcaaacattt tgtagacccg ttcataactt tcaacgatcg ggggatacat tgttgttctg
    10441 tattcgatct tcaccccttt tgaattttca aggatctctt ttgttttaag gagcttctgc
    10501 ttcacctcgt cctttgtgag catgcatcca agctttgaat agagatcagg cgtggtttta
    10561 atgtctattg caatatgcga aagataaggg agcgcctttt caatgaattc aggtcgcaag
    10621 ccgtttgaat caattttaat ttccagtttg aacttttctt ttatctcttt cagaaaacct
    10681 aaaaattcct catacagagt gggttctccg cctgaaaatg aaactccgtc aatgaatttt
    10741 gatctttttt caatttcgct aagaacatat tcctttgaaa ggatggcttc tttgtttacc
    10801 tcgaaaagtg aagagttgtg acagtaaggg catctaagat cacagccggc aaaaaagaaa
    10861 actgtggcga tctttcctgg aaaatcaata agagaagtct gttggtgacc ggtgatgggg
    10921 agcatttttt tcctccaaaa aactgttaac tgcaggatgt tatcacaaag gaaaaaaaaa
    10981 taaagtttga gggagcttca aaccattaaa taaattgata tggatcaaaa gaatggtata
    11041 atcaggccgt tgttgaatca gttcaagagg tgaatcatga gaaatcttgt tcttaagatg
    11101 gttactgaag gcgttctcac ttcggaaaat gccaacaaga tgcttgcaat gaaaaaagga
    11161 gttgaccctg tatggagagc tctttcaaaa gggttgatag atgaaaagaa gctggcaaga
    11221 ttctatgaaa aagaagggta tccggtcatt tatgatgaag gaagagggcc gatgtcggat
    11281 gatttcttta ctagattttt tacacctgat gtcatcgcac gccatctggc cgttcctata
    11341 tcattcaaga aagagagccg ggatataata gtaggtttta taaatgcggc gcttattcca
    11401 cagataaaag agaacatcct taagatcttt cctgaattca acaccacttt ttttcacata
    11461 ccttactcta tatttaaaaa gatcgcggct aaacactttc tttttgatat agacaaatat
    11521 acctctgtca tgatccagat ggaggttagc ggggaaatgc attcagataa agtggtgtcg
    11581 gtatcgtcag tcagaaaaaa actgcgtgaa gccgccgaac aggtctatat gcttgaaatt
    11641 gatgcacagg gatcagtcgt tttcaaagag gagtcgatga caagcaggtt ccctctcaag
    11701 tcacttccta ttttcaaaga aggttttgaa aggggatata tggagataat cccgggagac
    11761 aaccttaatt cattgagcca cactgaaaaa atattgctgt ttacaaatgt cgggataaag
    11821 aatgacgaca agataatgat acttgcggca gatgaagaaa aaaagacatt tgttcttctg
    11881 ctaaacccca aagttcctaa agatcagata gattttcttt taagataaaa atataatcca
    11941 gttaattcag ccacttaact ctgttctgat taaaagtgaa aaaaataagt tgacagaaag
    12001 ggggggaatc tttatattgt tcgagcgctt ttttgaggtc attgaaaact gagcagcagg
    12061 aagacaa
//
LOCUS       DAOOYB010000075        12008 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_5571_length_12008_cov_11.170919, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000075 DAOOYB010000000
VERSION     DAOOYB010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12008
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5571_length_12008_cov_11.170919"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            120..2243
                     /locus_tag="PL056_05975"
     CDS             120..2243
                     /locus_tag="PL056_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46598.1"
                     /translation="MKRLTGLTFSIIIFLTYFVIATKGRIYTIFTMFFPEFHIILLIS
                     VLFFGIIYAFFPKTEVKDDNRLVKSLFFIIPFSVFAFSLFINLFFIIAPVPDDGIHYV
                     WLSKLILNGKLYLETPDFYEHYYSNFLFNHNGNYVSIFLPGFSFFMTPFTFFGIEFCF
                     NPVIAGINTYLAGIHAVKLKDRSAGIVVMLMFGFSSVHLLHGGFYFPHHFGLMLVLIA
                     VYLIVHRAEKRSSFIIAGALLSIMLFIKPQNALYTYFAGAVYIIFKERSVKNAVLFTF
                     PFLVTGSALMLYNYHLTGNPFVFAQDVYFNALDLREFCHRPGFGKGCAGNHGETLPPE
                     GVTFVYATGITFLRLNNFLHRITAHPAILLFIIPVIFKKPYKYFLYYFMPLCAIFAYF
                     TFYIEGNFVGPRYLLESGALFVIAAGCGFSEIYCFFRNRKGIRNQFSAGSFAGIFAST
                     LIFFAFFVMPQMLVKTDPPDNLKKVKTLIEKEEIENSIILLPHSFGFHFHSILSVQDN
                     PPHDKYGNLIIYSLGELLDSNIQKFYKDSKYDKIVKVNKNANNFSIMEIPFLEDDGKY
                     HAEFEMKFIPLKGKPKFILGFYHIHQAQTFEFNPTPDVEFSYGAQGVLFDSQEEFYEF
                     EHSVKTSGNYKIDIVVVETGCTVKFSVLVNGLQKTEFIPEKGEISLKKITFTSQLNAG
                     KNTVRIIPHNEGCLILDYMDVSRSP"
     gene            2411..2851
                     /locus_tag="PL056_05980"
     CDS             2411..2851
                     /locus_tag="PL056_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018474514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPM46599.1"
                     /translation="MKVITIKNETDFPGQFGRSQLVDFMHESLDEFRDSKKDINNALD
                     YAFSTDPGKGGFVLLGIDNGNIVSGLVMNKTGHVGYIPENILVYVATKASERGKGLGR
                     KIITKAIETAGTDIALHVEYDNPAKKLYERLGFTSKYAEMRYKK"
     gene            2855..3961
                     /locus_tag="PL056_05985"
     CDS             2855..3961
                     /locus_tag="PL056_05985"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HPM46600.1"
                     /translation="MSKIIVNIPKIIENINEISQLMKKNKKEWTIVCKVLAGNREILR
                     NILHSKVIKKVHSVADSRLSGLKIMKEINPEIRTMLIKPPSKYQIPSVVRFADISLNT
                     SIKTIHSLNREAEKIGVVHKVIIMIELGELREGILKENTVKFYSSVFDLKNIEIIGIG
                     TNLGCMYGVEPDHDKLLQLTLFRKIIELKFGRDLPVISGGSSITLPLLELGAIPEGMN
                     HFRIGEAAFIGTTPYNNKRFHNLNEDGFRLSANIIEIEKKPLFPDGRIGNGNVGHADM
                     GLFKENSKENDHFKAIVDFGALDVSPETDLTPLDEKVRFIGTTSDMTVFDIGKNKVGL
                     KVGDTIKFKPNYMGVARLMLSKYVKKEPVDNINH"
     gene            3972..4532
                     /locus_tag="PL056_05990"
     CDS             3972..4532
                     /locus_tag="PL056_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46601.1"
                     /translation="MKLFLLTNLIFLLLFSCSTAQKKESAGQGGADRHELSSYYDEGD
                     NDKMAESSSKRDHLSSLAKECREGDGDSCYRAGSIYFQGRENEKSYKKAGKYFFEACK
                     LDHARGCYVLGMMFNNAVGTKRNCKKADDFFMRGCELDDENACVYLWECDESKEKGRF
                     YFSEDEKDEDYDRSDSYDEFSEYSDW"
     gene            complement(4533..6116)
                     /locus_tag="PL056_05995"
     CDS             complement(4533..6116)
                     /locus_tag="PL056_05995"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPM46602.1"
                     /translation="MQLADFITYSIFAGMTIAFLAIIWSYAKGLRQSPRDMWLMFIYN
                     IIEYTAYMAMNIAVTLWLTADCGVGDLAAGNYIMLWSILLSIIGMITGAVVDTIGIRK
                     TLMISIIFLLISRFFMSFITDPYIIFVLGFIPMAIGFAIVGPLISVAIKRFTTKDTVA
                     MGFGLFYVLMNLGYALGGLMFDGVRDVFSLRDSAGKIINENAGIDILGLHFSTYQMFF
                     VIGFLFTIVSMIVAFFIRGNIELNDDGKLTEVAKKELGSGFKAVRNSAIDVWNMISKV
                     VAEKMFWVYIGMLALTLFVRFVFFHFSYTFPKYGISILGEGAKIGNIYGVLNSFLIIF
                     TVPVVAYLTKKTASYKMLIIGSIVSSLSCFIAVIPSRFFVSLTDTVLGEMIFVKWLGL
                     ADSAQALSVNPPTPEYWPLIFFILIFTIGEAIWSPRLMQFTAEIAPKGKEGTYIALSI
                     LPWFAAKFFVGPMSGLLVKTYTPFQDGHPLPPADNYTMVWVWIGGMALLTPVTLLLFG
                     KYFMKILVPEKVAGNTSEN"
     gene            complement(6155..6766)
                     /locus_tag="PL056_06000"
     CDS             complement(6155..6766)
                     /locus_tag="PL056_06000"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HPM46603.1"
                     /translation="MISSQVAFFADLGSKLILVLLFVASFFVVAFYVERKLFFRKHFN
                     SDMPLLLKRLNSSPGRQDIETVLRSDKFTESEIVMKALSNTKVNSGEKLLSTVTTEID
                     MEKKNWEKFLLYFATVGSNAPFLGLLGTVLGLMQSFSDLALAARPDAKVAMAGISNAL
                     ITTVAGIVIAIPSVIIYNMLSKKVATVSANIKAIARTVADTIF"
     gene            complement(6769..7047)
                     /locus_tag="PL056_06005"
     CDS             complement(6769..7047)
                     /locus_tag="PL056_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019254016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b5 domain-containing protein"
                     /protein_id="HPM46604.1"
                     /translation="MACASTPYKETRAVPDKSFTVAELAKFDGKEGRRAYVAYKGVVY
                     DFTDVKSWKNGEHKDGKKAGNDYTDVLDKVWHGAKVLKDKPVVGKLVK"
     gene            complement(7136..7211)
                     /locus_tag="PL056_06010"
     tRNA            complement(7136..7211)
                     /locus_tag="PL056_06010"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7176..7178),aa:Thr,seq:cgt)
     gene            complement(7314..8546)
                     /locus_tag="PL056_06015"
     CDS             complement(7314..8546)
                     /locus_tag="PL056_06015"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPM46605.1"
                     /translation="MERFFELSRQFIAAGNKDFRRYFLEKNDFSHRFNIISGQRGVGK
                     STAMIQILAEYSKGDLFSNKILYVPSDHFLVAGSHLYTIAEEFYKLGGEFICFDEIHK
                     YKEWSMELKSIYDSFPDLKIIASGSSALEIHKGTHDLSRRAIVYKIDGLSFREFLTIR
                     ENVDLNTVTVDEILKDHEKLSFFINETLKNKNIRILPSFYEYLRIGFYPYFLQFKEEN
                     LMHIMLEQNIHTSIESDLTSVYSFINGETVRKIKKLLAVVASLVPYTPDLNSLKEICG
                     VSDHRTLKTYLSYIEDAGIISQYQKSGNEMDSLRKPEKIYLNNTAQMYAISPDNSKNI
                     GNIRETFFSQAVSPVHKLRIPKKGDFEIDGDLIVEIGGKSKKTGQLDNLKKSIYALDE
                     INSGFKNKIPLWLFGFLY"
     gene            complement(8681..10225)
                     /locus_tag="PL056_06020"
     CDS             complement(8681..10225)
                     /locus_tag="PL056_06020"
                     /inference="COORDINATES: protein motif:HMM:NF015530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46606.1"
                     /translation="MSKANEAISKFKKVDISYDYSTLPADIKNALPHLKKAFDLITTI
                     FMRQQMEGLPEIYNQVMAGNDEEKKEFFKLFKGPWDQLADFKSVYPEFSDRPATCAMY
                     PADITKEEWEKTLAEMSAEEKELMTDTYTVIVREEGKLKAVPYHVYYAEELGEIFEHL
                     EAAADCVENELLKEYLISRAEGMVTGNYRDSEALWVKMKDVPIDPVFGPFEVYADGFL
                     GLKATYEAFLMVVDHEKGNRLKEIENNLANLAAIFPVPEGSKAAVGKIAPMIVVHQIY
                     SSGEAAQGIMASAFNLPNDARVRGNVGWKQIMIYNMMQAKFNNATMKIADKISHGKIV
                     AEFDPYFYFVLMHEVSHGLGPAYRADGTPVSKAIGKNYTGIEEAKADIGGLYILLKLG
                     GKYGIPEFGKEKILNSFFAGLFRSMRFGVHEAHGAANVLEFNFFKENGIIKVLENGDF
                     ETDSTRLVEVTETLLNELCRIEANATPEEAAAFLKRFAVPGSDILDALAKLTDIPIDI
                     RVEYKL"
     gene            complement(10238..11941)
                     /locus_tag="PL056_06025"
     CDS             complement(10238..11941)
                     /locus_tag="PL056_06025"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HPM46607.1"
                     /translation="MTVATLLILFISSIISGCSDNKTNQTDNEGVISDEDGYVPLCGN
                     GFLDETEFCDSKSAKCSLLEPEKYRAGIATCLEDCSGYDFSKCLSVYECGNSIVEPGE
                     VCDGNFKLCSEIDPDKYSMGFASCFGHCNGWDSSKCKDFHMCGNEFLEQMETCEIGQT
                     KECAILYPSIFATGEAVCNEYCTEWNISSCIPLMCNDVKCGSIPIEIDGTDHIFNCGT
                     CFGGDICNSSNRCEFPCSDSKCGEVKIVDSDGITQTFECRECSGMQYCDTDNQCKAAC
                     SDMACGTDKGIDCGTCPDGYYCGPFPNRCKPKPKIEFTDIPGGTFFMGCNDVLDNNCA
                     ESERPGHIIELSPYSISTYEITVAQYETCIEAGFCRMDNPEYLDYKTYILNFRCNIGS
                     PRGAAHPANCISYYGAEAFCKFIGARLPTEAQWEKAARGGCEFYENCEAETQIFPWGN
                     EMATCDTAVMMDALSGIPGCETGGTMFVGSKPMGVSPYGLFDMSGNAWEWTSDWFDPG
                     YYNNSLEKDPQGPETGREKVLKGGSCNFSSNAIRPSYRYNVYPNVNYTYGGFRCVKTH
                     N"
BASE COUNT         3589 a         2416 c         2377 g         3626 t
ORIGIN      
        1 ccttcaaaaa tcagtaaaac aacaaagtat agcagaaatc cgtcagatta aaatatattg
       61 tgtctccttg aaaaaaagtg aattaaagat aaactgctaa aaactttccg gaaggtgccg
      121 tgaaaagatt aacaggttta acattttcaa taataatttt cttaacctat tttgtaatag
      181 ctacaaaagg gcggatatat accattttta cgatgttttt ccctgaattt catataattc
      241 tccttatatc ggtgcttttt ttcggaatca tttatgcatt cttcccgaaa actgaagtca
      301 aagatgacaa ccggctcgtc aagtcgctgt tttttataat tcctttttct gtctttgcat
      361 tttccctgtt tataaacctt ttttttatta tcgcacctgt gcctgatgac ggaatccact
      421 atgtctggct ttcgaaactt atcttaaacg ggaaactata ccttgaaacc cccgatttct
      481 atgaacatta ctactctaac tttcttttta atcataacgg gaactatgtg tcgatctttc
      541 ttccgggttt cagttttttc atgactccgt tcacattttt cgggattgaa ttctgcttta
      601 atcccgtaat tgcagggata aacacatatc ttgcaggaat tcatgcagta aaattaaaag
      661 acagatctgc cggaatagtt gtaatgctca tgttcggatt ttcatcagtc catcttcttc
      721 atggaggatt ttatttccct catcatttcg ggttgatgct tgttctgatc gcagtatatc
      781 tgatcgtcca cagagctgaa aaaagaagtt ctttcataat tgcaggagca cttctttcaa
      841 taatgctttt cataaagccg cagaatgcgc tttacactta ttttgccggt gccgtctata
      901 taatttttaa agagagatca gtcaagaacg ctgttctttt cacatttccg tttcttgtca
      961 ccggttcagc gttgatgctt tataattacc atctgacagg gaatcctttc gtttttgctc
     1021 aggatgttta tttcaacgcc cttgacttaa gagaattctg tcatcgtccc ggatttggca
     1081 aagggtgtgc cggcaatcac ggtgaaacac ttccgcccga aggtgtgact tttgtgtatg
     1141 cgacaggaat aacatttctg cgacttaaca actttcttca tcgaattact gcacatccgg
     1201 caattctgct tttcattatt cctgttatat ttaaaaaacc ttataaatat tttctctatt
     1261 atttcatgcc attatgtgca atttttgcat attttacttt ttatattgag ggaaatttcg
     1321 taggccccag atatcttctt gaatccggtg ctctttttgt tattgcggca ggttgcggat
     1381 tttcagagat atactgcttt ttcaggaaca gaaaagggat caggaatcag ttttcggctg
     1441 gctcatttgc aggaatattt gcttcgacac ttatattctt cgcatttttt gtaatgccgc
     1501 agatgcttgt aaaaacggat cctccagaca atttaaaaaa agtgaagaca cttatagaaa
     1561 aagaagagat cgaaaattca ataattcttc tccctcacag tttcggtttc cattttcatt
     1621 caatcttaag cgttcaggac aacccgcctc acgataaata cggaaatctt atcatttatt
     1681 ctcttggcga gctcctggac agtaacattc agaaattcta caaggactca aaatatgata
     1741 aaattgttaa agtgaataaa aatgctaata atttcagtat aatggaaatc ccattccttg
     1801 aagatgacgg gaaatatcac gctgaatttg aaatgaaatt catcccttta aaaggaaaac
     1861 cgaaatttat tcttggattc tatcatatac accaagctca gacatttgaa ttcaacccga
     1921 cgcctgatgt agaattcagt tacggcgctc agggtgttct ttttgacagt caggaagaat
     1981 tttatgagtt tgaacacagt gttaaaacca gtggaaatta caaaattgac atcgttgttg
     2041 tcgaaaccgg ctgtactgtt aagttttcag ttcttgtgaa cgggttgcaa aaaacagagt
     2101 tcatccccga aaaaggagag atatctttaa aaaaaataac tttcacatca caactcaatg
     2161 caggtaaaaa taccgttagg ataattcctc acaatgaagg atgtcttatc cttgattata
     2221 tggatgtttc tagatctcct taaaacggcc atatcgaatt gacaaagatg ccattttgtg
     2281 gtactatgtt tgaagtgatc tttttatgga ggtgctttaa ctatctgaaa agtctcaaaa
     2341 atttgttttt taattgagtc tcgatcttct gattttctga agcattcatt attgtttgga
     2401 ggcaaaaaga atgaaagtta ttactattaa gaacgaaact gattttccag gtcagttcgg
     2461 aagaagtcaa cttgttgatt ttatgcatga atctcttgat gagttcagag attccaaaaa
     2521 ggatataaat aatgcgttgg attatgcatt ttcaactgat cccgggaagg gcggctttgt
     2581 ccttttggga attgataacg gtaacatagt cagcggtctt gtaatgaata aaacaggaca
     2641 tgtcggatat atacctgaaa atattctggt ttatgtcgca acaaaagcaa gtgaaagggg
     2701 aaaggggctc ggcaggaaaa ttattacaaa agcgattgaa acagcgggga ccgacatcgc
     2761 tcttcatgtt gaatatgata atcctgcaaa aaaactatat gaacggcttg ggtttacatc
     2821 aaaatatgca gaaatgaggt ataaaaaata aacgatgtca aaaattattg tcaacattcc
     2881 aaaaataatt gagaatataa atgaaatcag ccagttgatg aaaaagaata aaaaggaatg
     2941 gacaattgtc tgtaaagtgc ttgccggaaa cagagagatc cttagaaaca tccttcattc
     3001 aaaagtgatc aaaaaagttc attctgtcgc tgattcaaga ctttcaggat taaagataat
     3061 gaaagagatc aatcctgaaa tcagaaccat gctcataaaa ccgccatcaa aatatcagat
     3121 accgagtgtt gtaaggtttg ccgatatttc tctaaataca tcaataaaaa caattcattc
     3181 attaaatagg gaagcggaaa agataggtgt cgttcacaaa gttataataa tgatcgagct
     3241 aggtgaatta agagaaggaa ttctcaaaga aaatacggtt aagttctatt caagcgtctt
     3301 tgatcttaaa aacattgaaa taatcggcat cggaacaaat ctcggttgta tgtacggagt
     3361 cgaacccgat catgataagc ttcttcagct cacgctgttt aggaaaatta ttgaactgaa
     3421 attcggaagg gatctgccgg tgatatcagg cggaagctcc atcacacttc ctctgcttga
     3481 actgggcgca attccggaag gcatgaatca tttcaggatc ggagaagcgg cgtttattgg
     3541 aacaacacct tataataaca agagattcca caacctgaac gaggacgggt tcagattgtc
     3601 agccaacata atagagatag aaaaaaaacc tttgttcccg gacggcagga ttggaaacgg
     3661 aaatgtgggg catgctgata tgggtctgtt caaagaaaat tcaaaagaga atgaccattt
     3721 taaagcaata gttgatttcg gagctttgga tgtaagcccc gaaactgact taacaccgct
     3781 tgatgaaaaa gtgagattta tcggtaccac ttccgatatg acagtgtttg acataggaaa
     3841 gaacaaagtc gggctcaaag tcggagatac tatcaaattt aaaccaaatt atatgggggt
     3901 cgcacgatta atgctctcaa aatatgttaa aaaggagccg gtagataata ttaaccatta
     3961 aggaggcggc gatgaagcta tttttattaa caaatttaat ttttcttctt cttttttcct
     4021 gttcaacggc acagaaaaaa gagagtgcgg ggcagggggg ggccgatcgt catgaattgt
     4081 caagctacta tgatgaaggg gacaatgata agatggcgga gtcttcttca aaaagggacc
     4141 atctgagttc tcttgccaaa gagtgcagag aaggggacgg agattcctgt tatagggcag
     4201 ggtctatcta ttttcaggga agagaaaacg aaaaaagtta caaaaaagcg ggaaaatact
     4261 tttttgaagc atgtaaactt gatcacgcaa gaggatgtta tgttctcgga atgatgttta
     4321 acaatgctgt gggaacaaag agaaattgca aaaaagcgga tgattttttt atgcgcggat
     4381 gcgagctcga tgacgaaaat gcatgtgttt atctctggga atgtgatgaa tcaaaggaaa
     4441 aagggcggtt ttatttcagt gaagatgaaa aagatgagga ttatgaccgc tctgacagct
     4501 atgatgaatt cagtgaatat agtgactggt agttaatttt ctgaagtatt gcctgcaact
     4561 ttttcaggga ccagtatctt catgaaatat ttgccgaaaa gaaggagtgt gacaggtgtc
     4621 agcagcgcca ttcctcctat ccagacccag accattgtgt agttgtctgc cggtggaagg
     4681 ggatgtccat cctgaaaagg ggtatatgtc ttgacaagaa gtcccgacat cggaccgaca
     4741 aagaattttg cggcaaacca gggaaggatg gaaagtgcga tgtaagttcc ttcttttccc
     4801 ttgggtgcga tctctgccgt aaactgcata agccgtggtg accagattgc ttcaccgatc
     4861 gtgaaaataa gtatgaaaaa tataagaggc cagtattcgg gagtcggcgg atttaccgag
     4921 agagcttgag cagaatctgc aagtcccagc catttaacga atatcatctc accgagcaca
     4981 gtgtcagtaa gagaaacaaa aaatctcgaa gggataacgg caatgaagca cgaaagcgaa
     5041 gaaacgatcg aaccgatgat gagcatttta tatgatgccg ttttcttggt taagtatgca
     5101 acgacaggaa cagtgaaaat tatgaggaat gaatttaata cgccgtaaat attgccaatt
     5161 ttagcacctt cgccaagaat gctgatcccg tatttgggga atgtgtatga aaaatggaaa
     5221 aatacgaacc ttacaaaaag tgtcagtgcg agcattccga tataaaccca gaacattttt
     5281 tcagcgacaa ctttagatat catattccag acatcaattg cagaatttct tacagcttta
     5341 aatccgctgc cgagctcttt tttagcaact tctgtcagtt ttccgtcgtc attcagttca
     5401 atattgcccc ggataaagaa tgcaacaatc atgcttacaa ttgtaaaaag aaatccgatg
     5461 acaaagaaca tctgatatgt ggaaaaatgg agcccgagaa tatcaatacc ggcattttca
     5521 ttgataattt tcccggcaga gtcacgcaga gagaacacat cccttacacc atcgaacatc
     5581 aatccgccca gagcataccc gagattcatc agaacataga aaagcccgaa acccatagcc
     5641 acggtatctt tggtcgtgaa ccgttttatg gcaacggata tgagtggacc aactatcgca
     5701 aaacctattg ccatcggaat gaacccgagc acaaaaatga tatacgggtc tgtaatgaaa
     5761 gacatgaaaa accgggatat caaaagaaaa atgatgctta tcatcagagt ttttctaatg
     5821 ccgatcgtat caacgaccgc tccagtaatc attccgatta ttgaaagaag gatcgaccag
     5881 agcataatgt agttgcctgc cgcaagatct ccgacaccgc aatctgccgt aagccacagt
     5941 gtcacagcaa tgttcatcgc catataggca gtgtattcga tgatgttgta tatgaacata
     6001 agccacatat cacgcggact ttgtctcaac cctttcgcat agctccagat aattgcaaga
     6061 aatgcgattg tcattcctgc aaaaatggaa taagtaataa aatcagccaa ctgcatcaag
     6121 acacctcagg tatttatgtt aaaaaaatat tcaatcagaa aatcgtgtca gccacagttc
     6181 ttgcaatggc ttttatgttg gcggaaacag ttgcaacctt tttggacagc atgttatata
     6241 ttattactga agggattgcg atgacaattc ccgcaacagt tgtaataagg gcattgctta
     6301 ttcccgccat ggcgactttg gcatccggtc ttgccgcaag tgcaagatct gaaaatgact
     6361 gcattagacc cagaactgtt ccaagaagtc caagaaaagg ggcatttgac ccgacagtgg
     6421 caaaatagag aaggaatttc tcccagttct ttttttccat gtcaatttca gtggttacag
     6481 ttgaaagaag tttttctcca ctgttcactt tagtatttga aagggctttc ataactattt
     6541 ctgactctgt gaatttgtca gatcttaaaa cagtttcaat atcctgtctt cccggtgaac
     6601 tgttcagccg ttttaaaaga agaggcatat cgctgttaaa gtgttttctg aaaaacagct
     6661 ttctttcaac ataaaaagca acaacaaaga aacttgccac aaaaaggaga acaaggatca
     6721 gctttgaacc cagatcggcg aaaaatgcaa cttgagaact tatcatggct atttaaccag
     6781 cttaccgaca acgggcttat ctttgagaac ttttgcaccg tgccacacct tgtcaagaac
     6841 atcggtatag tcgttccctg ctttcttgcc gtctttatgt tctccgttct tccatgattt
     6901 aacatcggtg aaatcataga caacgccttt gtatgccaca tacgctcttc ttccttcctt
     6961 accgtcaaat tttgcaagct cggcaacagt gaaagatttg tcaggaaccg cccttgtctc
     7021 cttgtacggg gttgaagcac atgcaatgaa aattactgcc accacgatga acgcaaaaac
     7081 tgaacctgca atgatcttct ttttcatgat aaccctccaa aaagattgaa aaaactggag
     7141 cttccgagcg gaatcgaacc gctgacctac tgattacgaa tcagttgctc taccgactga
     7201 gccacggaag caccatcgcg gaattataga aattcggcgt gagttgtcaa agttatcaat
     7261 aaataaaatg gcagtgttcc cagtttagca aaaatcttct ttaaaaacca ttactaatac
     7321 agaaatccaa agagccacag cggaattttg tttttaaagc cggaattaat ttcatcaagg
     7381 gcgtaaatgc ttttttttag attatctaac tgtccggttt tcttgctttt tccgccgatt
     7441 tcaacaataa ggtcaccatc aatttcaaaa tctccttttt tcgggattct cagtttatga
     7501 accggtgaaa ctgcctgcga aaagaaagtt tcccttatgt ttccgatgtt ttttgaattg
     7561 tcaggcgata ttgcatacat ttgagcagta ttattgagat atatcttttc aggttttctc
     7621 agtgaatcca tttcatttcc cgacttctga tactgtgaga taattcctgc atcttcaatg
     7681 tatgaaagat atgttttcag cgttctgtga tctgaaactc cgcaaatttc ctttaaagag
     7741 ttcaaatcag gcgtatatgg aacaagtgaa gcgacaacag caagcagttt ctttattttt
     7801 ctgacagttt ctccatttat gaaactatat accgatgtca gatccgattc aatcgaggtg
     7861 tgtatgttct gttccagcat aatgtgcatc agattttctt ctttaaactg taaaaaataa
     7921 gggtagaatc cgatccgcag atattcataa aaactcggca gaatgcggat attcttattt
     7981 ttaagggttt cgttgataaa aaaggagagc ttttcatgat cttttaatat ttcatcaact
     8041 gtgacagtgt taagatcaac attttccctg atagtaagaa attctctaaa agaaagcccg
     8101 tcaattttat aaacgatcgc ccgtcgcgaa agatcgtgtg ttcctttatg aatttcaaga
     8161 gcgctgcttc ccgaggcaat tattttaaga tcaggaaatg aatcataaat actttttagc
     8221 tccatagacc actctttgta tttatgtatt tcatcaaagc agataaattc tccgccaagt
     8281 ttataaaatt cttcggcaat agtatagaga tgacttcccg caacaagaaa atgatctgac
     8341 ggaacataga gaattttatt tgaaaacaga tcccccttgg aatattcagc cagaatctgt
     8401 atcatggctg ttgattttcc gacccctctt tgacctgaaa taatgttaaa tctgtgcgag
     8461 aagtcatttt tttcaagaaa atatctccta aaatccttgt tcccggcagc aataaactgt
     8521 ctgctcagtt caaaaaacct ttccatcata acctccaaat taggattgga ttaccatcac
     8581 acacagtcaa ttatggtatt ccattacaat aattcaagta aaaaattagt attggattac
     8641 tattttgatt gttttttttg tgaaatcgtt tgaaaatcaa ttacaattta tactcgactc
     8701 tgatatctat ggggatgtcg gtgagtttgg cgagtgcgtc aaggatgtcg cttccgggga
     8761 cggcgaaccg tttgaggaat gctgctgctt cttcgggtgt ggcattggct tcaatgcggc
     8821 agagttcgtt aaggagtgtt tcggtgactt caacgagtcg tgttgagtcg gtttcaaaat
     8881 cgccgttttc aaggactttg atgatgccgt tttccttgaa gaagttgaat tcaaggacat
     8941 ttgcggctcc gtgtgcttcg tgtactccga atctcattga tctgaagagt cctgcaaaaa
     9001 agctgtttaa gatcttttcc ttgccgaatt cggggattcc gtattttccg ccgagtttaa
     9061 gaagtatgta aagtccgccg atgtcggctt ttgcttcttc tattccggta tagttcttgc
     9121 cgatagcttt tgaaacaggt gtgccgtcag cacgatatgc aggtccaaga ccgtgactta
     9181 cttcatgcat gaggacaaaa tagaagtagg ggtcaaactc tgcaacaatt ttgccgtgag
     9241 atattttgtc agcgatcttc attgtcgcat tgttgaactt tgcctgcatc atgttgtaga
     9301 tcataatctg tttccagccc acatttcctc ttacccgcgc atcgttaggg agattgaacg
     9361 cacttgccat gatcccctga gccgcttcac cgcttgaata gatctggtga acaactatca
     9421 tcggagcgat cttgccgaca gccgcttttg aaccctctgg aaccggaaat attgccgcaa
     9481 gatttgccag attgttctca atttccttta aacgattccc tttttcgtgg tcaacgacca
     9541 tgagaaatgc ttcataagtt gctttaagtc caagaaatcc gtctgcatat acttcaaaag
     9601 gaccaaacac cggatcgatc ggaacatctt tcatttttac ccacaaggcc tcggaatcac
     9661 gatagtttcc agtcaccatt ccttcagctc ttgagatgag atactctttc aggagttcgt
     9721 tttccacaca atctgccgca gcttcaaggt gttcaaagat ctcaccaagt tcttcagcat
     9781 aatagacatg ataggggact gctttgagtt tgccttcttc acgaactatg acggtatatg
     9841 tgtcagtcat aagctccttt tcttccgcac tcatttctgc aagggttttc tcccattctt
     9901 ctttagtaat atcggcagga tacattgcgc atgtcgcagg cctgtcgctg aattcgggat
     9961 ataccgactt aaaatcggca agctgatccc acggcccttt gaaaagcttg aaaaactcct
    10021 tcttttcctc atcattgccg gccatgacct gattgtaaat ctcaggaagt ccttccatct
    10081 gctgacgcat gaagatcgtt gtgatcagat cgaatgcttt tttcaggtgg ggcagggcgt
    10141 ttttaatatc agccggtaaa gttgaatagt cataactgat atcaactttc ttgaatttac
    10201 tgatcgcttc gtttgctttt gacatttttt cctccggtta gttatgagtt tttacacagc
    10261 ggaatccgcc gtatgtatag ttgacattgg gatagacatt atatctgtaa cttggtctta
    10321 tagcatttga gctgaaattg caggaacctc ctttcagcac tttttctctc cctgtttcag
    10381 gaccctgagg atctttttcc aaagaattgt tgtaatatcc cggatcaaac cagtcacttg
    10441 tccattccca tgcatttcca gacatatcaa aaagtccgta cgggcttaca cccatgggct
    10501 ttgaacccac aaacattgtt ccaccggttt cgcatcctgg aataccgctg agagcatcca
    10561 tcattacggc agtatcgcat gttgccattt catttcccca tggaaatatc tgcgtttcag
    10621 cttcacagtt ttcatagaat tcgcaaccgc ctcttgccgc tttttcccac tgtgcctcag
    10681 taggcagccg ggctccaatg aatttacaga atgcttcggc accgtaatag ctgatgcaat
    10741 ttgcaggatg agcggcacct cttggagatc cgatgttaca cctgaagttg aggatatatg
    10801 ttttgtagtc aagatattcc ggattatcca ttctgcaaaa ccctgcttcg atgcaggttt
    10861 cgtactgcgc aacagtaatt tcataagtgc ttatggaata gggacttaat tctataatgt
    10921 gtccgggtct ttcactctcg gcacaattat tgtcaagcac atcgttgcaa cccatgaaaa
    10981 aagttccgcc gggtatatcc gtgaattcta ttttcggctt tggcttacat ctgttcggga
    11041 aaggaccaca gtaatatcca tccggacatg ttccgcaatc aattccttta tctgttccgc
    11101 aagccatatc tgagcaggcg gccttacatt gattatctgt gtcgcaatac tgcattcctg
    11161 aacattctct gcattcaaaa gtctgggtta taccgtcact gtcgactatt ttcacttcac
    11221 cgcatttgct gtctgagcag gggaattcac atcgatttga gctgttgcag atgtctccgc
    11281 cgaagcatgt tccgcaatta aaaatatgat cagtgccgtc aatctcgatc gggatactgc
    11341 cgcatttaac atcattgcac atcaatggaa tacaactgga aatgttccat tccgtacaat
    11401 attcgttgca taccgcttcc cctgttgcaa atatggaagg atacagtatt gcacattctt
    11461 tggtctgacc gatctcacag gtttccatct gttcaagaaa ttcattgccg cacatatgaa
    11521 aatccttgca ttttgaagaa tcccatccat tacagtgccc gaaacaggac gcaaaaccca
    11581 ttgaatattt atcaggatct atttcactgc atagtttaaa attcccgtca cagacttcgc
    11641 ccggctccac aatggagttg ccgcattcat aaacggaaag gcatttagaa aagtcatagc
    11701 cggaacagtc ttcaagacat gttgcgatcc ctgctctgta tttttcaggt tcaaggagtg
    11761 aacattttgc tgatttagaa tcacaaaact ctgtttcatc aagaaagccg ttgccgcaca
    11821 aagggacata accgtcttca tctgaaatga cgccttcatt atccgtctga tttgttttgt
    11881 tgtcagaaca tcctgatatt attgatgaaa taaagagtat cagtaaagtg gcaactgtca
    11941 ttttcttatt taagttcaaa ccagtaggaa tagtttttaa aagtagtatt tactttgaca
    12001 ttggttat
//
LOCUS       DAOOYB010000076        11947 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_5625_length_11947_cov_12.042297, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000076 DAOOYB010000000
VERSION     DAOOYB010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11947
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5625_length_11947_cov_12.042297"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            83..718
                     /gene="gmk"
                     /locus_tag="PL056_06030"
     CDS             83..718
                     /gene="gmk"
                     /locus_tag="PL056_06030"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006003864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HPM46608.1"
                     /translation="MEENGHLYHGKIFVLSAPSGAGKTTLVKNIKERFPFLVESISCT
                     TRAPRKNEVDGVDYFFLTVEEFIKRENAALFAETAEVHGNFYGTPLNFLKERITEGKN
                     IICDIDYQGAINIQKAFPDESVLIFILPPSMEELEKRLRKRATDNEDTVQKRLENARN
                     EITYYTFYNHIVVNDNFETASIQLEHIIRAYTESSVLHNLETIKRLTGENG"
     gene            711..>2326
                     /locus_tag="PL056_06035"
     CDS             711..>2326
                     /locus_tag="PL056_06035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HPM46609.1"
                     /translation="MDRTELKTVLKRDPYIKLPKVIRPSHIIDAASEQYSMMKEEHIE
                     EILEKIHKAIAVASSAHEGVVRKNGEPYIFHPYAVAYLLAKMGMDADCIIAGLLHDTV
                     EDTTTTVKQIESMFGRSVANLVEGVTKLTEMKLAQEEKQAIAFKKLITFAKDDIRIIP
                     IKLLDRLHNMMTLDAMPEEKQKRISSETMRFYAPLAHRLGFYWLKEELESLSFYFGMK
                     DEWKMIDSFIENKYPNISETIQKLIGKVGEAIEIKSPDLSEKIYDIYGRAKSYFSIYK
                     KTIRKNLEISSLHDILGVRVILGDENKDDCYLAMAAIHSYPEFTIINKYFKDYISRPK
                     ENGYMSIHTVVRYKEFFIEVQFRSKEMDRVAREGNASHWAYKNDVSHKDKVAKWLEEV
                     LCDFTDSDNAIEFMSGIENVLPLDAIAVFSPKGDIFPLPDGSTLLDFAYAIHGDVGDQ
                     CIGGTVNGKRVTINHPLHSKDEVRVETSKNQVPRKDWLTFVKTAKAKQYIKRALSKKE
                     KEATENRGREILKPLFLSQGRANDFENLRD"
     assembly_gap    2327..2426
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2427..2846
                     /locus_tag="PL056_06040"
     CDS             <2427..2846
                     /locus_tag="PL056_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46610.1"
                     /translation="IKDYIIAGCCQPVQGDNVSTYISPTRGYILHKSNCPSLAGFSSE
                     RIEKDVYWFEYSEYTIEFIVKLKNNKGALLELVEELTFRDFNIMSMHLNPEDAFEKLG
                     NVYVSVKGSKIHEIESLSRNLKSKKSIIDLIISNIDY"
     gene            2862..3731
                     /gene="dapA"
                     /locus_tag="PL056_06045"
     CDS             2862..3731
                     /gene="dapA"
                     /locus_tag="PL056_06045"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HPM46611.1"
                     /translation="MKGVYTAVITPFDKAGNVDFKSLEKILETQVEGGVSGIVAAGTT
                     GEAATLTLDEKIELFSFVKEKCPGLDIVAGTGSNNTSDSVKQTEAALKTGINKVLIVT
                     PYYNKPTANGLVRHYGEIGKTGAEMILYNVPGRTGLNVAPEALAKLAAIDNIVAVKEA
                     TGDLGRFADYLDAVGSERFFFLSGDDFTIAPFISMGGTGVISVVSNVLPELTVALVNA
                     ALSGDFKKAADIQISMNRLNHAMFMESNPGPVKTALSLMGLCEDNFRSPLDKMEEKNI
                     NKLSEILKDYHLI"
     gene            complement(3745..5076)
                     /locus_tag="PL056_06050"
     CDS             complement(3745..5076)
                     /locus_tag="PL056_06050"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HPM46612.1"
                     /translation="MKITRIALISAVIFCSLIVFSSCGKSKKKTIDDNDVSDIDAIDE
                     DVIEDEETVDDGEIIHDDDIPLNDPDLDIADDDLPVDNDSVIEPDDGVADDLQDTDVV
                     DDSDQQDDSDQNDTSILPDEDTDEDADEEIADVDPYEPVVACTGMSKCYNNSAEMTCP
                     ATGDFYGQDHQYAVLGVCIAKSFTVSGTAPQEVVADDLTGLIWQRTLPSIYPGCTGGL
                     TSGETCSWQQAVDYCEGLDYGGFTDWRLPSVEELSTLPDYGKYIPAIDSDVFPETDPS
                     YFWTSSVYAVLNTYVWLTYFNNGDVDRYLKTKYYYARCVRGEQYKNGSVFTKITLTGQ
                     DVIKDERTGIIWADTYATAKTWQEALKYCEDMDYAGSTGWRLPNVNELKSLLDYSLRN
                     PATSFPAMPDQFFWSSTSYLGYGEYGWYVNFTYGEVYYHTKTNYYHARCIR"
     gene            complement(5158..5871)
                     /gene="rnc"
                     /locus_tag="PL056_06055"
     CDS             complement(5158..5871)
                     /gene="rnc"
                     /locus_tag="PL056_06055"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HPM46613.1"
                     /translation="MALDLELVRELEKKLRYEFSDQKLLEKAFTHPSSEVSGNDHYQR
                     LEFLGDEVVGLGVSALLFKTFPDAEEGKLSKARSNLIDEPGLAFNARSLELGPLLILG
                     KGEEKMEGRDKDSILADSFESLMAVIFLEAGWMTAFRIITDVFAPLINASPDIEDLLD
                     HINRDYKTRLQEIAQELELPLPVYNVIGKTGPEHDLMFVIECSAVGFTGTGTGRNKKS
                     AEQDAAMKILREMRIISSK"
     gene            complement(5871..6602)
                     /gene="rlmB"
                     /locus_tag="PL056_06060"
     CDS             complement(5871..6602)
                     /gene="rlmB"
                     /locus_tag="PL056_06060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HPM46614.1"
                     /translation="MAKSSVEYVYGINSCFEVIRGGKRKIYQVYLGEKNVNNPRIKKL
                     IEVLDKKEIPHEWVTKERLNQLSGSTDNQGAVMKTSLYKYEGLESVIGAKRILLLDNV
                     EDPHNVGAVLRSAEIFGFKSVLLPVRGVPEIYPSVIKVSAGASEHLNIVRESASNVYV
                     KKLQEEHGYTVVALDGEGKSTLDDVKNINPEKILLVIGGEDRSVGQFILNKADFVVAI
                     KQFGKINSLNASVAASIAMYALGGK"
     gene            complement(6681..7217)
                     /locus_tag="PL056_06065"
     CDS             complement(6681..7217)
                     /locus_tag="PL056_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46615.1"
                     /translation="MNKRNIIMGLMLIAMIGIFYFKESSVEKNVSKNIEKESVKLFEN
                     FDASSLRSIDITNLKKGEKVTFEKSGDNWIVKDKNCNADKNAIMRIIETLPKIRLGRD
                     MGAFSPDHMNEYGFDKGLEIQAGGKVIMLGDQKGVSIALKDGNNIYLAPFGEKYVFEK
                     HDGNWCEKTEEKKPETAE"
     gene            complement(7217..8833)
                     /locus_tag="PL056_06070"
     CDS             complement(7217..8833)
                     /locus_tag="PL056_06070"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HPM46616.1"
                     /translation="MKNKRRDAVISVVLATAIAIFINILATDLFYRLDFTKGGLYTIS
                     SSTKEILSKLNDDVEIKFYVSEELPPKVLPIKRNVLDLLTEYERYGGGKVTLSVLYPE
                     KSADIEKDAKSAGIQKVQLNVIGQNKEELQAAYMGIVLFFKDKMETLPVVLSVNDLEF
                     QLTSLILKLAKDKKERITFLNSKPQLPPDLDPQMRAQLAAQMPQSYSINQDTQAVAQA
                     LRELYHVEEIRLEKGENFKEDTKIVVLHEPANLSGWEKFAVDQFLMKGGKVIVLQSGM
                     KVSQQGFAQEKQSNYDDMFKSYGFDIQKNMVMDVYNYSVMIPQGNMRYLAPYPLWIKI
                     PPENISSDLPESIRNAGTLAFTYTSSINLNKVEDIDYKSIVKSSGKSWAQSSMVMLDP
                     SAIKEPSMSDLKVFDLAVLGQGKFKSAFTKDTLPEEAKDRGSDLIEKGIADGAVFYIA
                     APEFILDRTVQNFQSNGIFFINMVDYLTNSTDLVNIRSRGQGFVHISPEITDNAKNII
                     KWTGTLLVPFIVIVFGLIRMISRNRRSGRA"
     assembly_gap    8909..9008
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9009..9430)
                     /locus_tag="PL056_06075"
     CDS             complement(<9009..9430)
                     /locus_tag="PL056_06075"
                     /inference="COORDINATES: protein motif:HMM:NF024741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPM46617.1"
                     /translation="MKRALVIAKKELLSYFNSAIAYIFFTLFLVILGFLFFRLYFLIG
                     VVSMNDFFSIIPWIFLIFIPSVTMKSWAEEKKTGTMEVLFTMPVTDLVLVTGKFLAGF
                     AFVSIAIILTFIVPVLVSMTGTLDWGPVITSYIGLFLLA"
     gene            complement(9430..10356)
                     /locus_tag="PL056_06080"
     CDS             complement(9430..10356)
                     /locus_tag="PL056_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013252981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPM46618.1"
                     /translation="MDVIIELANVSKSYNEIKAVENLSFKLKKGEILGFLGPNGAGKT
                     TAMKMITGYMPPDTGTIKVKNEDILKDPVKARISIGYLPENNPLYLDMTVAEYLNFIA
                     EMRGIENREKAIDSALAKCGIKNVSHRIINHLSKGYKQRVGLAQAIIHDPEILILDEP
                     VNGLDPKQITEIRNLIKEIGKEKTVILCSHILYEVEAVADRVLIINNGRIAADDTIEN
                     LRERSKGSNIFVLDIIDETADIAEKLNTLENVKKVEKVHSGLYRIFAAGNKETGHSIF
                     VLAASNNWRVVQLYLEGNSLENMFLNLTEGGK"
     gene            complement(10434..10724)
                     /locus_tag="PL056_06085"
     CDS             complement(10434..10724)
                     /locus_tag="PL056_06085"
                     /inference="COORDINATES: protein motif:HMM:NF027117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin, RelE/StbE family"
                     /protein_id="HPM46619.1"
                     /translation="MNNIVLKFKESASKEFSKIPKQLQPKIYDEIQSLKENPLKGKKL
                     KGKLSAFRRIRIGKYRVAYKFIHCEIVIIVVKIGDRKDFYEQLSRLVQDMRK"
     gene            complement(10727..11170)
                     /locus_tag="PL056_06090"
     CDS             complement(10727..11170)
                     /locus_tag="PL056_06090"
                     /inference="COORDINATES: protein motif:HMM:TIGR02384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HPM46620.1"
                     /translation="MRESTISARMDSEMKKKVESILNKLGLSHSSAINLFYSQILMNE
                     GLPFEMKLTGKKTKNRKIDVVAEPEITYNQTDTLFESMEDLPSDLIKMISRFGKSKKA
                     KAAIVKLAVEFFLEELEFIEECEQRMNDDNDPLISEEEFKKRFGL"
     gene            complement(11254..11877)
                     /gene="udk"
                     /locus_tag="PL056_06095"
     CDS             complement(11254..11877)
                     /gene="udk"
                     /locus_tag="PL056_06095"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HPM46621.1"
                     /translation="MPLFIGIAGGSGSGKTTIARKIKKSFSKGERVIILEMDSYYKDL
                     HGKSFEEREKNNYDIPDALDFELLEKQLKTLLENKPIQKPVYDFTIHLRKDETELVEP
                     ADIIILEGILTFHKKEIRDLLDIRIFVDTDADIRLLRRIRRDMEQRGRSFEEIRERYS
                     CMVRPAYRDFVKPTIQHSHLVIPEGGQDFVAIDMIVSKITHWLMEKK"
BASE COUNT         3424 a         2507 c         2271 g         3545 t
ORIGIN      
        1 aataacaatc aagttgaaat agaaaaaatt ctgctatata atcaccggac actatttgtt
       61 atacttttta ccggagtatg ttgtggaaga gaacggtcat ttataccacg ggaagatttt
      121 tgtactttca gctccttccg gagcggggaa aacgacactt gtaaagaata ttaaggagcg
      181 gtttccattc cttgttgaat caatttcatg cactacaaga gctccgagaa aaaatgaagt
      241 tgacggggtc gattatttct ttctgacagt tgaagagttc ataaaaagag aaaatgcagc
      301 cctttttgca gaaactgccg aggttcacgg aaatttttac ggaacaccgc tcaattttct
      361 caaagagagg ataactgaag ggaagaacat tatctgcgac atagactatc agggtgcgat
      421 aaacattcag aaagctttcc cggacgaatc ggtgcttatc ttcattcttc cgccatcaat
      481 ggaggaactt gaaaaaagac tgcgcaaaag agcgacagat aacgaggaca cggttcagaa
      541 acggcttgag aacgcaagga acgaaataac ttattacact ttttacaacc atattgtggt
      601 gaacgacaac tttgaaacgg cgagcattca gcttgaacac atcatcagag cttataccga
      661 atcttcagtt ctacacaatc ttgaaacaat taaacgactt acaggtgaaa atggatagaa
      721 ccgaacttaa aaccgttctg aaaagagacc cttacataaa actgccgaaa gtcatacggc
      781 ccagtcatat aatcgatgcg gcaagcgaac aatacagtat gatgaaggaa gaacatattg
      841 aagagatact cgaaaagatc cacaaggcca tcgctgttgc ttcatcagcc catgaaggag
      901 ttgtgagaaa gaacggtgaa ccgtatattt ttcaccctta tgccgttgca tatcttcttg
      961 caaaaatggg aatggatgcg gactgtatca ttgcagggct tcttcatgac acagttgaag
     1021 acacgaccac gaccgtaaag cagatcgagt ccatgttcgg ccgttcggtc gcaaatcttg
     1081 ttgaaggggt tacaaaactt acagaaatga aactggcaca ggaggaaaag caggcgatcg
     1141 catttaaaaa gctcatcact tttgccaaag acgacatccg gataatcccg ataaagctgc
     1201 ttgaccgtct tcacaacatg atgacgcttg atgcaatgcc ggaagaaaaa cagaaaagga
     1261 tatcgagcga aacaatgagg ttttacgctc cgctcgcaca tcgtctcgga ttttactggc
     1321 ttaaagagga actggaatcc ctgtcattct atttcggcat gaaagatgag tggaaaatga
     1381 tcgacagttt tattgagaac aaatatccga atatatccga aactattcag aaacttatcg
     1441 gaaaagtcgg agaggcgatc gagataaagt cacccgacct ttctgaaaaa atatatgaca
     1501 tctacggcag agctaaatca tatttcagca tttataaaaa aacgatcagg aaaaatcttg
     1561 agatatcaag tctccatgac attctcggag tgcgtgttat ccttggtgac gaaaacaagg
     1621 acgactgcta tcttgccatg gcggcaattc attcatatcc tgaatttaca ataatcaata
     1681 aatattttaa ggattacatt tcaagaccca aggaaaatgg atacatgtcc attcacaccg
     1741 ttgtcaggta caaggaattt tttattgaag ttcagttccg ctccaaggaa atggaccgtg
     1801 tcgcaaggga aggcaatgct tcccactggg catacaaaaa tgatgtttcc cacaaggata
     1861 aagttgcgaa atggcttgaa gaggtacttt gtgacttcac tgactcagac aacgccattg
     1921 aatttatgtc ggggatagaa aatgttcttc ctcttgacgc aattgctgtg ttttctccaa
     1981 aaggggatat tttcccgctt cctgacggat cgaccctgct tgattttgca tatgcaattc
     2041 acggtgatgt cggcgatcag tgtataggcg gaacagtcaa cggaaaaaga gtgaccatca
     2101 atcatcccct tcacagcaag gatgaggtca gggtcgaaac atcaaaaaat caggtcccga
     2161 gaaaagactg gctcactttc gtaaaaactg ccaaggcaaa acaatatata aaaagagctc
     2221 tttcaaaaaa agaaaaagaa gcaacagaaa atagagggag agaaatactt aagccgcttt
     2281 tcctttcgca gggcagagca aatgattttg aaaatctcag ggatttnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnatca aagattatat aattgccggg tgctgtcagc
     2461 cggtacaggg agataatgtc tccacatata tttctccgac gagaggatac attcttcaca
     2521 aaagcaattg tccgtcatta gccggttttt caagcgaaag gatagaaaaa gatgtctact
     2581 ggtttgaata ctcggaatat actattgaat tcatagtcaa acttaaaaac aataaggggg
     2641 cgcttcttga acttgttgag gaattgacct tcagagattt caacataatg agtatgcacc
     2701 tcaatcccga ggatgcattc gaaaaactgg gcaatgtcta tgtctcggta aagggctcca
     2761 aaatacatga gatagaaagt ctgagcagaa acctgaaaag taaaaaatct attatcgatc
     2821 tgatcataag caacattgac tactaaactg gggaggagtg aatgaaaggg gtctacacag
     2881 cggttataac tccgtttgac aaagcgggaa atgtcgattt taaatcactg gagaaaattc
     2941 ttgaaactca ggtcgaaggt ggcgtttccg gtatagtagc cgccggaaca acaggtgaag
     3001 cggcaacact tacgcttgat gaaaagatag agctgttcag ttttgtcaaa gaaaaatgtc
     3061 cggggcttga tattgttgca ggaaccggct caaacaatac atctgattca gttaaacaga
     3121 ctgaagcggc gctcaaaacc ggcataaaca aagtgctgat agttacacca tattacaaca
     3181 aaccgacagc aaatggtctt gtcagacatt atggtgaaat tgggaaaaca ggtgccgaaa
     3241 tgatacttta caatgttccg ggcagaaccg gtctgaatgt ggctcctgaa gcactcgcaa
     3301 aacttgcggc aatagacaac attgtggctg ttaaagaggc gacaggcgac cttggcagat
     3361 ttgcagatta tcttgatgct gtgggaagtg aaagattttt cttcctttcc ggtgatgact
     3421 tcacgatcgc tcctttcatt tcaatgggtg gaacaggtgt gataagcgtc gtctcgaatg
     3481 ttcttccaga gcttaccgtg gcacttgtga atgctgcttt atccggcgat ttcaaaaaag
     3541 ccgccgacat tcagatctcg atgaaccggc tcaatcacgc gatgttcatg gaatccaacc
     3601 cgggaccagt caaaactgct ctttcattaa tgggtctgtg tgaagataat ttcagatccc
     3661 cgcttgataa aatggaagag aagaatatca ataaattatc ggaaatattg aaagattacc
     3721 atctgatctg aatttcaggc aactttacct gatacacctt gcgtgataat aatttgtctt
     3781 tgtgtgataa tatacttcgc cgtatgtgaa attgacatac cacccatatt caccgtatcc
     3841 cagatagctg gttgacgacc agaaaaactg gtcgggcatt gccggaaaag atgttgcagg
     3901 atttcttaaa gaataatcta gaagtgattt cagctcattg acattgggaa gccgccagcc
     3961 cgtagagccg gcataatcca tatcttcgca gtatttcaat gcctcctgcc atgtttttgc
     4021 agtagcataa gtatccgccc agatgatgcc ggtcctttca tctttaatta catcctgacc
     4081 ggtcaaagtg atcttggtga acacactgcc gtttttatat tgttcgcccc tcacacatct
     4141 tgcataataa tatttagttt tgagatatct gtcgacatca ccgttattaa aatatgtcag
     4201 ccagacatat gtgttgagga cagcgtatac agacgatgtc cagaaatagc tcggatcagt
     4261 ttcagggaaa acatcagaat caatggcagg gatatatttt ccgtaatcgg gaagtgttga
     4321 cagttcttca actgacggaa gccgccagtc tgtgaaaccg ccgtaatcaa gaccctcgca
     4381 gtaatcgact gcctgctgcc agctgcatgt ttcaccggaa gtcaatcctc cggtacatcc
     4441 cggatagatg gaaggaagag ttctctgcca gatcagaccg gtcagatcat cagccacaac
     4501 ttcctgcggt gctgttcctg aaacagtgaa gctttttgca atgcaaacac caagcactgc
     4561 atattggtga tcctgcccgt aaaaatctcc agttgccgga catgtcatct ctgctgagtt
     4621 attatagcac ttgctcattc ctgtacatgc aacgaccggt tcataaggat caacatctgc
     4681 aatttcttca tcggcatctt cgtctgtgtc ttcatccgga agaatggatg tgtcattctg
     4741 atcagaatca tcctgctgat cagagtcatc aacaacatca gtatcctgca aatcgtcagc
     4801 aacaccatca tccggttcga taacactgtc attgtcaacg ggaagatcgt catctgcgat
     4861 atccagatca ggatcattca acggaatgtc atcatcatga attatctcac cgtcatcaac
     4921 agtttcttca tcttcgataa catcttcatc gatcgcatca atatcagaaa catcgttgtc
     4981 atcaattgtt tttttcttcg attttccgca acttgaaaaa acaataagag aacagaatat
     5041 cacagccgaa attaacgcta ttcttgttat tttcactgcc tcaccccttg gaaataaaag
     5101 ttaaaaaact tgaaaaacac aaatatcata caacatttac aggaattttt caatttgtta
     5161 tttggaactg ataattctca tttcccttaa gatcttcatt gcggcatcct gttcggctga
     5221 tttcttgttt ctgcctgtgc ctgttcctgt gaaaccgact gctgaacatt ctatcacaaa
     5281 catcaggtcg tgttcaggtc ctgttttgcc gataacatta tataccggaa gaggaagttc
     5341 gagttcctga gctatttcct gcaatcttgt tttataatca cggtttatgt ggtcaagcag
     5401 gtcttcaata tcgggagagg catttatgag gggggcaaaa acatctgtaa ttattctgaa
     5461 agctgtcatc cagcccgctt caagaaaaat gacggccata agagattcaa acgaatcggc
     5521 aagaattgaa tccttgtctc tcccttccat cttttcttca ccttttccaa gtatcagaag
     5581 cggtccgagc tcaagagatc ttgcgttgaa agcaagtccc ggctcatcaa taagattact
     5641 tctggctttt gaaagtttac cttcctcggc atcaggaaaa gttttgaaaa gaagggccga
     5701 cacaccaaga cctacaactt catcaccgag aaattcaagc cgctgataat gatcattgcc
     5761 tgaaacttcg gaacttggat gggtaaatgc cttttcaaga agtttttgat ctgagaactc
     5821 atatctcagc tttttttcaa gttccctgac aagttcaaga tcaagagcca ttattttcct
     5881 ccgagagcgt acattgcaat gctcgccgcc actgaagcgt tgagcgaatt tatctttccg
     5941 aactgtttga tggcaacgac aaaatcggct ttgttgagta tgaactgacc gacagatctg
     6001 tcttcgcccc ctatcaccag aaggatcttt tcaggattga tatttttaac atcatcaaga
     6061 gtgcttttcc cttctccgtc aagagctaca accgtatatc catgttcttc ctgaagtttt
     6121 ttcacataaa cattggaagc cgattccctg acgatattca gatgttcact cgctcctgca
     6181 ctgactttta ttaccgatgg atatatttca ggaactccgc ggacaggaag aagaacgctt
     6241 ttaaacccga agatctcagc actcctgagc acagcaccca cattatgggg atcttcaaca
     6301 ttatctaaaa gcagaatcct ttttgcaccg atgacacttt caagtccttc atatttgtat
     6361 agggaggttt tcatcacagc gccctgatta tcggttgaac cggaaagctg attgagccgc
     6421 tcttttgtga cccactcatg aggtatttcc tttttatcaa gaacttcaat aagttttttt
     6481 attcttggat tatttacatt cttttcacca agatatacct gataaatttt tctttttcca
     6541 cccctgatca cttcaaaaca ggagttgatc ccgtaaacat attcgacaga tgattttgcc
     6601 ataataccgc ctttaaaaaa aagaatcagt caacggtttg cattgtattt aatttgtaaa
     6661 tagtgtcaaa ctggctttat ttattcagca gtttcgggct ttttttcttc tgttttttca
     6721 caccagttgc cgtcgtgctt ttcaaataca tatttttcgc caaatggtgc gagatagata
     6781 ttgtttccat ctttaagtgc aatagaaact cctttctgat caccgagcat tatgactttc
     6841 ccgcccgcct ggatctcaag tcctttgtca aaaccgtatt cattcatgtg atcaggtgag
     6901 aatgctccca tatccctgcc gagccggatc ttcggaaggg tttctatgat cctcatgatc
     6961 gcatttttat ctgcattgca gtttttatct ttaactatcc agttgtcgcc gcttttttcg
     7021 aaagtgactt tttccccttt tttcaggttt gtgatatcaa ttgatctaag cgatgacgca
     7081 tcaaagtttt caaacagttt aacagattcc ttttcaatat tctttgagac atttttttca
     7141 acggagcttt ctttaaaata aaatataccg atcatagcaa ttaacattaa tcccatgata
     7201 atattgcgtt tattcattat gccctccccg atcttctgtt ccttgagatc attctgatca
     7261 gaccgaaaac aattacaatg aacggaacaa gcagtgttcc tgtccacttg atgatgttct
     7321 ttgcattgtc tgttatttca ggactgatat ggacaaagcc ctgtccgcga cttctgatat
     7381 ttacaagatc ggtgctgttt gtcagataat cgaccatatt gatgaagaat attccgtttg
     7441 actggaaatt ctgcacagtt ctgtcaagta tgaattcagg ggccgcaata tagaagacag
     7501 ctccgtcagc aattcctttc tcgatcagat cggaacccct atcttttgcc tcttccggca
     7561 gggtatcttt tgtgaaagca ctcttgaact ttccctgacc gagcactgca agatcaaaaa
     7621 ctttaagatc gctcattgat ggctctttta tggcagaggg atcgagcatg accatgcttg
     7681 actgagccca gctctttcct gacgacttta caattgactt gtaatcaata tcttcaactt
     7741 tgttaagatt tatggaactt gtatatgtaa aagcaagggt tccggcattt cttattgatt
     7801 ccggaagatc agatgaaata ttttcgggtg ggatctttat ccacagagga taaggtgcaa
     7861 gatatctcat attcccctga ggtatcatca cagaatagtt atacacatcc attaccatgt
     7921 tcttctgaat gtcaaagccg taactcttga acatatcatc ataatttgac tgtttttcct
     7981 gcgcaaatcc ctgctgtgat accttcattc ccgactggag aacaataact tttccaccct
     8041 tcatcaggaa ctggtcaact gcgaattttt cccagccgct cagattagcc ggctcgtgga
     8101 gaacaactat tttcgtgtct tccttaaagt tctccccttt ttcaagacgg atctcttcaa
     8161 catgatataa ttcccgcagt gcctgtgcca ctgcctgagt atcctgattt attgaatagc
     8221 tttgaggcat ctgtgccgca agctgagccc tcatctgcgg atcaagatcc ggtggaagct
     8281 gcggttttga attgagaaaa gttatccgtt cctttttatc ttttgccagc ttcaatatca
     8341 gcgaagttag ctgaaattca agatcgttca ccgaaaggac gacagggaga gtttccattt
     8401 tatccttgaa aaaaagcacg atgcccatat atgccgcctg aagctcctct ttgttctgcc
     8461 cgatgacatt aagctgaacc ttctggatac ctgcactttt tgcgtctttt tcaatatcgg
     8521 cgcttttttc aggatagaga acagaaagtg tcacttttcc gccgccgtat ctttcatatt
     8581 cagtaagaag atcaagtaca tttctcttaa tcggaagaac tttcggtggt agttcctcgc
     8641 tcacatagaa ttttatttcc acatcgtcat tgagttttga aaggatctct tttgtcgatg
     8701 aacttattgt ataaagtccc ccttttgtaa aatcaagccg gtaaaaaaga tcggttgcaa
     8761 gaatgtttat gaaaatcgcg atcgctgttg caagaacaac gcttattact gcatctcttc
     8821 ttttgttttt cattgttccc tcctaccagt ttctgctttc aatcgagcgg atgttgaaga
     8881 acatgaaaag ggaaatgata gaaagatann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9001 nnnnnnnngc cagaaggaaa agcccgatgt atgaagtgat cacgggcccc cagtcaagag
     9061 ttccggtcat tgagacaaga accggcacga tgaatgtgag gattatcgcg atcgaaacaa
     9121 aagcaaagcc agccagaaat ttccctgtta caagaacaag gtcagtgacc ggcattgtga
     9181 aaaggacttc cattgttcca gtctttttct cttcagccca gcttttcatt gtgacagagg
     9241 gaatgaagat gagaaatatc catggaataa tgctgaaaaa atcattcatg ctcacaactc
     9301 cgatcaggaa ataaagccgg aaaaacaaaa atccgagtat cacaaggaac agagtaaaaa
     9361 atatgtaggc aatcgctgaa ttaaaatagc tgagcagttc tttttttgca attacaagtg
     9421 ctcttttcat tatttccctc cttctgtaag gttaaggaac atattttcca gtgaattccc
     9481 ttcaagataa agctgaacga ccctccagtt gtttgatgcc gcaaggacaa atatggaatg
     9541 accagtttct ttgtttcctg cggcaaatat tctgtaaagt ccgctatgaa ctttttcaac
     9601 tttcttcaca ttttccaaag tgttgagttt ttcagcaata tcagcggttt catcaataat
     9661 atcaagcaca aagatgttgc tgcccttgct tctctcacgg agattttcga tggtgtcgtc
     9721 cgctgcgatc ctgccgttat ttattatcag aactctgtca gcgaccgctt caacttcata
     9781 aagtatatga ctgcacagaa ttacggtctt ttcctttccg atctctttaa taaggtttct
     9841 tatttcagtt atctgtttgg ggtcaagtcc gttcaccggt tcatcgagaa taagaatttc
     9901 agggtcatgg attatcgcct gggcaaggcc cactctctgt ttgtaaccct ttgaaaggtg
     9961 gttgatgatt ctgtgagaga catttttgat gccgcatttt gccaaagccg agtcaatcgc
    10021 tttttcacgg ttttcaattc ctctcatttc tgcaatgaaa ttaagatatt cggccactgt
    10081 catgtcaaga tagagcggat tgttctcagg gagataaccg atgcttatcc ttgccttcac
    10141 cggatctttt aaaatgtctt cattctttac tttaattgtt ccggtgtcag gcggcatgta
    10201 accggtgatc attttcatgg cagttgtctt tccggctccg tttggcccca gaaatcctaa
    10261 aatttcacct tttttcagtt tgaaagaaag gttttcaact gctttgatct cattgtaact
    10321 ctttgaaaca tttgcaagtt caataattac atccataaaa ccctccaaaa aaaatcaaaa
    10381 ttcgtatcta taacagatta tttttgaaaa ttattccaga aaaataagaa aaattatttt
    10441 ctcatatcct gaacaagcct ggatagttgt tcgtaaaaat cttttctgtc accgatttta
    10501 actacaataa taacaatctc gcaatgaatg aatttgtatg caaccctgta ttttccaatc
    10561 cttattcttc tgaaagcgct taattttcct ttgagctttt ttcctttcaa cggattttct
    10621 tttaaagatt gaatttcatc gtaaatcttt ggttgaagct gtttggggat tttgctaaat
    10681 tccttcgagg cagactcttt gaattttaaa acgatgttat tcaattttat aacccaaatc
    10741 tttttttgaa ctcttcttca cttatcaacg ggtcattgtc atcattcatc ctttgctcac
    10801 attcttcaat aaattcaagt tcttcaagaa aaaactcaac tgcaagttta actattgcgg
    10861 cttttgcttt ttttgatttt ccgaatcttg agatcatttt aattaaatcg cttggcagat
    10921 cttccatact ttcaaataat gtatctgtct gattataggt aatttcaggt tctgcaacca
    10981 catctatttt tctgttcttt gttttcttcc ctgtcaattt catttcaaaa ggaagtcctt
    11041 cattcattag aatctgactg taaaaaaggt ttattgcaga agagtgcgaa agccccaatt
    11101 tattaaggat gctctccacc ttcttcttca tttcagaatc cattctcgca gaaattgttg
    11161 actctctcat aaacttacct ccatcgcaaa ttgacaaaat aattgtacta cgaagttgca
    11221 caatgtcaag aataagccgg tttttttatg cttctacttc ttttccatga gccagtgagt
    11281 tatttttgag acgatcatgt caattgcgac aaaatcctgt cctccttccg ggatgacgag
    11341 gtgtgaatgc tgaattgtcg gctttacaaa atcgcggtat gcaggtctga ccatgcatga
    11401 atatctttcc ctgatctctt caaatgatct tcctctttgc tccatatcgc gacggatcct
    11461 tcttaaaagc cggatgtcgg catcggtgtc aacaaagatc cttatgtcaa gcaggtcacg
    11521 gatctctttt ttatggaaag tgagtatccc ttcaagaatt attatgtctg ccggttcaac
    11581 aagttcggtt tcatcttttc tgaggtggat ggtaaaatca tagaccggtt tttgaattgg
    11641 cttgttttca agcagcgttt tcaactgttt ttcaagaagt tcaaaatcca gggcatcggg
    11701 aatatcatag ttgttttttt ccctctcttc aaagcttttt ccatgcaagt ccttgtaata
    11761 actgtccatt tcaaggatga taacccgctc tccttttgaa aaggattttt ttatctttct
    11821 tgcgatcgta gttttacctg aacccgaccc gcctgcaata ccaatgaaaa gaggcatgat
    11881 aaactccaaa aaaaattaaa gtcacagcaa atatacaaag caccaatatt ttttcaagtt
    11941 gttacgc
//
LOCUS       DAOOYB010000077        11616 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_5857_length_11616_cov_9.397543, whole genome shotgun sequence.
ACCESSION   DAOOYB010000077 DAOOYB010000000
VERSION     DAOOYB010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11616
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5857_length_11616_cov_9.397543"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1592)
                     /locus_tag="PL056_06100"
     CDS             complement(<1..1592)
                     /locus_tag="PL056_06100"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HPM46622.1"
                     /translation="MRNIFIISIVILSFFQFMHSEESTGEVVLYVFSLGKPAKDLRAI
                     FDEKTVEFTDESGAARAVLNAGSHKVKVTGDKKVVAVAEFLVVSGESTRIIISAKNDG
                     TNSEIEIEHPEGSIEKVDENINKNLAPGVFSGVVTSLVDKSVVPQASIFIKGIRSRIV
                     TDESGSFKTELPPGSYAVSFIHAQFSTQTVEGVEIKEKLETVKNIELTPASIELDEYV
                     VLVPHIQGGIASLMEERKESSAVSDVIGAEQMSKSGDSNAASALKRVTGLTVVGGKYV
                     YVRGMGERYSSTLLNGSTLPSPEPEKRVVPLDIFPTGMLQSVEVQKTFTPDMPGEFGG
                     GVIKIKTKGIPEKFTASLSLSAGFADDSFSTGMSYKGGKYDWLGIDDGSRDIPKDVLD
                     ATKGDVALKEKDKYSKDGFTKEEMESLGESFPNRWNTHETMILPDLGFSITLGDRWKL
                     RDVSFGLLGSFSYSNGMETKQTTTNSYGFSGDDLASKSRYVYNVTTNDIKMGSILESG
                     LDIGKHTKIGNTFIILRTTEDAV"
     assembly_gap    1692..1791
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1792..2573)
                     /locus_tag="PL056_06105"
     CDS             complement(<1792..2573)
                     /locus_tag="PL056_06105"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:NF020132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HPM46623.1"
                     /translation="MYRNMLKPPAILIISGVDPTNGAGMGRDISTVRENDGFPISVPS
                     VLTIQNSMDFSGVIPVSFDYVSDSIDVLQDEFQIYSIKTGLLPLENEWILKLTEKIQN
                     FKVPVVIDPVFKATSSKVENLIVPDSYISFMAGDNKVITPNLKELKIIHRLLKNSEET
                     VEIMSENISKELGCCVVTTFEAGESFIFVTGRGVTTKIPIELFEPQRNIHGTGCTFSS
                     SLAVNLAKGIPLNESVGLSAQYTLEKVKKSVRYNKSGQYFLQ"
     gene            complement(2554..2700)
                     /locus_tag="PL056_06110"
     CDS             complement(2554..2700)
                     /locus_tag="PL056_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46624.1"
                     /translation="MNGVASVVASIFSIILAMAYGFTFVFTMAAFCYFAAIVAFLRFV
                     QKHA"
     gene            complement(2710..4974)
                     /locus_tag="PL056_06115"
     CDS             complement(2710..4974)
                     /locus_tag="PL056_06115"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46625.1"
                     /translation="MIFTGIFLISFGIIALQILQTRIFSFTLWHHLAYMVITVALMGM
                     AAAGTWLSIRKKQPVNPHRFLALCSSGFSISSVISLAVALRVPLDTFMPDKILQLAYI
                     FLYYLLLIFPYFFAGVAISFIFTFISKRINLYYMSNLAGSAIGGMAVLPVMEKFGAEG
                     AVFAVSLLGMLSALFFAISGRDRRSLIVSISGFILLAALFPLREDLMPAKPPASKALG
                     IGYTYDSGQKITHTKWDRVARIDVFENKHSQGFFNYFPDLQNKVITIDGDAYTLLYNF
                     PGGSADISGSLYSIGYHLHEKPKVLVVGLGGGTDIVTALHNGAYSVTGVEINKAMIDV
                     TKDLYADFIGKPYQDSRVNIVHSEGRSFIRRSPDKFDIIQMSGVDTWAALSTGAYVMS
                     ESFIYTTDAMREYLEHLTDDGTLSIIRWLFWPSREMLKLCTQAVQVLRERGVESPENN
                     IAVVGDGALAAILVKNRPFTWSELDHMNEVVSATENIRIIYAPGFSASAQYYDPVFAK
                     HNFSTSEGINYINSGFNAFFKAVEKGDEEKFISEYYYDIEPVSDDRPFFFKYYRVNHL
                     FSDVNRTGGSFVDSMPIGLLILGLSLFQALFFSFMFIVMPLFFMGKKERSFSPVNQII
                     YFFMIGTGFMFIEMSVIQQFVLFLGNPADAISVTIMILLLSAGIGSLLSRKILIAVGE
                     KGIFGAMLLIFPLIIMGYAVLLPNFTHKFIHYSFSARIVLSLVVLFPLGFLLGQFFPT
                     ALTIVGEKNSSFIP"
     gene            complement(4994..6514)
                     /locus_tag="PL056_06120"
     CDS             complement(4994..6514)
                     /locus_tag="PL056_06120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HPM46626.1"
                     /translation="MKRQIIVNAVHGEKRVAIIEEKKLVELHIMRSDTGYCLGNVYSG
                     IVKRVLPGMQAAFVEFGGTRTGFIFINDLRTDITYEEFQKTVQDDEIEESQENQSGHP
                     VKRDIAKYVKEGERVLVQVIKEPIGQKGARLTTHISLPGRFCVLLPTISHIGISRKIT
                     DNIKRNELKELGTKVQKKGYGIILRTLAGSSDVKIIEKEAVSLIKKWVEIQNAYKKEK
                     GAALIHDSLDPLTEIIQNTGIEDLKEIVTDSRLDEKNVRSYLQFYNENPDELVKFYDL
                     PYPIFDYYSIEPEITSVLKKKIWLKSGGFLIIEQTEALTVIDVNTGKFVGKGNLESTV
                     LKTNLEAVEEIAYHLRLRNIAGIIVVDFIDMRNQNNKKKVISELERELEKDPAKCSVF
                     YFTRLGLIQITRKRTTESNISSMTEPCPYCNGNGIIRSRDTMVFQMFRDIQREVKLYG
                     SRFINIKAHPDAAFALEHKHSEEFGELKKELKILVEVQTDNSFHREHYIVSSREKD"
     gene            complement(6528..7298)
                     /locus_tag="PL056_06125"
     CDS             complement(6528..7298)
                     /locus_tag="PL056_06125"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPM46627.1"
                     /translation="MLSILPLGCYGSDLENKKCISMQITPSTVIDTGSLMSSLDIPQL
                     LEIRNVIITHSHFDHIKNLPIFADFMLSLGNHVFTVYTTEKIMSEIKTHILNDLIWPD
                     FTKLPSKSKPTINFQPVEFEKPFSLDGIEFLPIEVNHLVESIGFIIRKNDCSIAYSGD
                     TFICDNFVNHVNDEPTVKAVCWETSFPNRLENIAVASKHLTPALLEKELLKLKRKNEV
                     HTFHLKPNLENEIIEDLKKVQADMPIIPMKQKRPLTVS"
     gene            7505..9412
                     /locus_tag="PL056_06130"
     CDS             7505..9412
                     /locus_tag="PL056_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HPM46628.1"
                     /translation="MFKNFTFLMLIPAILILISCNPEDQYLSKLYPKESRNYAIQSLK
                     KMKGEIGNLDKTCEKLLKLDRTPEAGVAVLAAGEIGCTMSIPRIGEIVDDCLVSLNVR
                     NLKTLESAAMAMGLLKNPDAVPILAKYFSINTPEVLAAGMREKAESVAKRAAIEALSK
                     MPNESKHLVPEILKVFNSSIEDFGTKYTTAGIMGEWQDPSTVRPLVTSLFYEEQGFSL
                     FPEARKSLIRLGKLAEEELIKAYNGQNPTVNDIQNANKDRATKQFCPEYIDNEEAKKK
                     GDCPKDDEFVATIASIETTTKLKTSIVLADIRSKLAVDMVIAELEAQLATDKKQPFLA
                     EHLAVQLAKFGDFKATDTLLKMVSKKFTKNAVKKPDHKPSKEEIAQAKLADRGQEISI
                     TMKGAEALAILGDPKSIPYLLELAKQKPISEYNEMNEKILFYESIVWGADALTRMMYE
                     PEMASDFIKHAESIISEGESYVAGVEKKTKEQILKETKGQELSAEDLDKKIKTQARLD
                     VNYDSTNKTVEMFKRFVSRAKVSQECGKEVKCYVEKLKDKDSAVVEKAVYMIGYSGEI
                     KHYKDQLKPVFTHVEPYVREALSIALLKTEDKGFVPIIEEVLKNEGDKVEYALAMREF
                     RAIASYLSSLN"
     gene            9456..11327
                     /locus_tag="PL056_06135"
     CDS             9456..11327
                     /locus_tag="PL056_06135"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HPM46629.1"
                     /translation="MKVAIVNVNRSRISRNTEYHLFTLKAFIDERAVSDIETEIINIY
                     MTDDMATTANLLKNKNADIILFSVLYWNSAYILKLAESCRTLPAIKGLWGHDTFSHPE
                     EYLKKDFDFIIQDEPELALYEMASLKKENRDISSASGIIFKDRVKKTFVFGENKVISD
                     LDLIPSPYLKEMIEVDEHTVVYWEVARGCSFKCDFCVEFSKAGNLRYHSFAYLEKELR
                     YFVSRNVSQIVIGCPIFNLSHQHFVKILDMLKFHLPHASVEIQARPDLLTREDIESLA
                     EMNVFINIGLQTVNQKVHENLMTSLNVENALNIIRYMANFPNLPFGIDIIAGLPKMTF
                     DDFLNDLENAFNLWPVSLNVFRLSMYPGTRMFNRMREYNYTVEHSYPYRALDNPQFNK
                     REFEKISEMAEGIDVLYNKGRMVSIITMLAKGLEMPCHEIITRWNKWIKKQPVDMNSM
                     DPDDINYDQLFEYINEFFSYLFDRFQKKKLWPVASDILKHNYFYTTSLMTLEEDIITY
                     PYQIEAIDNKTLIGINCSAFFDKFSYDIEDIIETGYIDLKKYSCDVDKESMYGLVYRL
                     DGSVFTKTITDEEGSIFSFIRKKNSVSISEIQKKFKNIDVLNFITFWCDEGVLFTVQ"
     gene            complement(11355..11430)
                     /locus_tag="PL056_06140"
     tRNA            complement(11355..11430)
                     /locus_tag="PL056_06140"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11395..11397),aa:Asn,seq:gtt)
BASE COUNT         3537 a         2391 c         2190 g         3398 t
ORIGIN      
        1 accgcatctt ctgttgtgcg cagaataata aaagtgttcc ctatttttgt gtgtttgccg
       61 atatcaagac ctgactcaag aattgagccc atttttatat cgtttgttgt aacattgtaa
      121 acatatctcg attttgaagc aagatcatcg cctgaaaaac cgtaactgtt ggttgttgtc
      181 tgctttgtct ccattccgtt gctgtatgaa aaagagccaa gaagtccgaa actcacatct
      241 cttagcttcc acctgtcacc aagagtaata gaaaacccca gatcgggcag aatcattgtt
      301 tcatgagtgt tccatctgtt cggaaaagat tcgccaagtg actccatttc ctcttttgta
      361 aaaccgtctt tactgtattt atccttttcc tttaatgcaa catccccttt tgtcgcatca
      421 agaacatctt ttggaatatc gcgagacccg tcatcaatgc ccaaccagtc atattttcca
      481 cctttgtaac tcattccggt tgaaaaagaa tcgtcagcaa aaccggcact taacgataaa
      541 gaagcagtga atttttcagg aattcccttt gtttttatct tgatcactcc gcctccgaac
      601 tctccgggca tatcgggggt aaatgttttc tgaacttcaa cgctctgaag cattccggtt
      661 ggaaaaatgt caagcggaac aaccctcttt tccggctcgg gactcggcag ggttgaaccg
      721 ttcaggagtg tgcttgaata tctttcgccc atacctctga catagacata tttgccacct
      781 acaactgtga gccccgtgac tctttttaaa gcagatgccg catttgaatc acccgatttt
      841 gacatctgct ctgccccgat aacatcgctc acggcacttg attcctttct ttcctccatt
      901 aatgaggcaa ttccaccctg gatatgaggc acaagaacga catattcatc aagctcaatt
      961 gatgccggcg taagttcaat attcttaaca gtttcaagct tttcctttat ctcaactcct
     1021 tcaactgtct gagtggagaa ctgtgcatga atgaatgaaa cagcataact gccgggtgga
     1081 agttcagttt tgaaggaacc cgattcatca gttacaatac gcgaccttat tcctttaata
     1141 aatatagatg cttgcggcac gacacttttg tcaacaagtg aagtgacaac ccctgaaaaa
     1201 actccaggtg caaggttttt gttaatattt tcatcaactt tttctatact tccttcagga
     1261 tgctctattt caatttcaga atttgttccg tcattttttg ctgagattat tatccttgtg
     1321 gattcaccgg aaacaacaag aaactccgca actgcaacaa cttttttgtc tcctgtaact
     1381 ttaaccttgt gtgatcctgc atttaaaacc gctcttgccg cacccgattc atctgtaaat
     1441 tcaactgttt tctcatcaaa gatcgctctc agatcctttg cgggcttccc aagagaaaaa
     1501 acataaagaa caacttcacc tgtactttcc tcagagtgca taaactggaa aaatgacaga
     1561 ataacgatag aaattatgaa gatatttctc aaacttaact ccggaaaaca gtttttaaac
     1621 aactacaacc ggatactatt taaattaaat ttgttaaaac agtgttagcg ttgtgtgaga
     1681 aagatgtttt gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntactgtaga
     1801 aaatactggc cggatttgtt gtatctaact gattttttta ctttttcaag tgtatattgt
     1861 gcagaaagcc cgactgattc gtttaacgga atcccttttg caagattgac tgccagagat
     1921 gaagagaatg tacagccggt gccatgaata tttctttgcg gttcaaacag ctcgatagga
     1981 atttttgttg ttacacctct gccggtgaca aaaatgaatg actcccctgc ttcaaatgtt
     2041 gtaactacgc agcatccaag ctcttttgag atgttttcag acattatttc cactgtttct
     2101 tcagagtttt taagcagtct gtgaataatt ttcaattcct ttaagtttgg agttattact
     2161 ttgttgtctc ctgccataaa tgagatgtaa ctatccggaa caattaagtt ttcaactttg
     2221 cttgatgtgg ctttgaaaac cgggtctatc actaccggca ctttaaaatt ctgaatcttt
     2281 tcagttaatt ttaatatcca ctcattttca agcggcagca atccggtctt gatcgaatat
     2341 atctgaaatt catcttgaag aacatcaatg gaatcagaca cataatcaaa tgagacagga
     2401 ataacacccg aaaaatccat tgagttctgg atcgtgagaa ctgaagggac cgatatcgga
     2461 aatccgtcat tttcccttac tgttgaaatg tcacgcccca ttccagcacc gtttgttggg
     2521 tcaacgcccg aaatgataag tattgcaggt ggcttaagca tgtttctgta caaatcttaa
     2581 gaaagcgact attgccgcaa aataacagaa tgccgccatc gtgaaaacga atgtgaaccc
     2641 gtaagccatt gcaagtatta tcgaaaatat cgaagcgaca accgaagcca caccgttcac
     2701 agcatacgct cacggaataa aactgctgtt cttttcacca acaatggtca gagccgtcgg
     2761 gaaaaactgt ccaagcagaa atccaagcgg aaaaaggaca acaagcgaca acactatcct
     2821 cgctgaaaat gaatagtgaa tgaacttgtg tgtaaagttc ggaagcagta ccgcataacc
     2881 cattatgata agcgggaaga tcagaagcat tgcgccgaat atcccctttt cgcctacagc
     2941 aataaggatt ttcctgctta aaagagaacc gatacctgcc gaaagaagaa gtatcattat
     3001 tgtcaccgat attgcatcag ccggatttcc aagaaacaga acgaactgct gtataaccga
     3061 catttcgatg aacataaaac ccgtccctat catgaaaaaa tagatgatct ggtttacagg
     3121 gctgaacgac cgttcctttt tacccatgaa aaaaagcggc attacaatga acatgaacga
     3181 aaagaaaagt gcctgaaaaa gactgagtcc gagtatcagc agacctatcg gcatagaatc
     3241 aacaaaagag ccgcctgtcc tgtttacatc ggaaaaaaga tgattgacac ggtaatattt
     3301 aaaaaagaaa ggtctgtcat ccgaaaccgg ctcaatatcg taataatatt cagaaatgaa
     3361 cttctcttca tctccttttt caaccgcctt gaaaaacgca ttgaaaccgc tgtttatata
     3421 atttatccct tcagaagttg aaaagttgtg ctttgcaaat actggatcgt aatattgagc
     3481 ggaagcggaa aatccggggg cgtatattat tcttatgttt tcagttgctg acaccacttc
     3541 attcatatga tcaagctctg accaggtgaa aggccggttt ttgacaagga tcgccgccaa
     3601 agcaccgtca cccacaactg caatgttgtt ttccggactt tcgacacctc tttcccgaag
     3661 cacctgaact gcctgcgtgc agagcttcag catttctctc gaaggccaga aaagccaacg
     3721 gattatactt aaagttccat catcagtaag atgttccaga tattccctca tcgcatcagt
     3781 cgtgtatatg aagctttcgc tcattacata ggcaccagtc gaaagtgccg cccatgtatc
     3841 aacccccgac atctggatta tgtcaaattt gtcaggactt ctgcggataa agctccttcc
     3901 ttcgctatga acaatattta cacgactgtc ctgatatggt tttccaatga aatcggcata
     3961 aagatccttt gtgacatcta tcattgcttt gtttatctct acacccgtaa ctgagtatgc
     4021 accattatgc agagctgtta caatatcagt ccccccgcca agacccacga caagcacttt
     4081 cggcttttca tgaagatggt aaccgataga gtaaagtgat cctgaaatgt ccgcactccc
     4141 tcccgggaag ttatataaaa gtgtgtaggc atcaccgtca attgttatca ccttgttttg
     4201 caagtctgga aaataattaa agaatccctg tgaatgtttg ttttcaaaaa catcaatcct
     4261 ggcaacccta tcccactttg tgtgagttat cttctgacct gaatcgtatg tgtaaccgat
     4321 tcctaacgcc ttggaagcgg gaggtttcgc aggcatcaga tcttctctca gaggaaaaag
     4381 agcggcaagt aaaataaaac cggatattga aacaattaag gaacggcgat ccctgcctga
     4441 tattgcaaaa aacagagcag acaacatacc cagcagcgaa acggcaaaaa cagcaccttc
     4501 agccccgaac ttctccatta caggaagaac cgccatgccg ccaatggcgc tacctgcaag
     4561 attcgacata taataaagat tgatcctttt acttatgaat gtgaaaatga aggaaattgc
     4621 cacacctgca aaaaaatacg ggaaaataag aagaagataa taaaggaaaa tataggcaag
     4681 ctgaagtatt ttgtcaggca tgaaagtgtc aagcggaacc ctcagtgcga ctgcaagaga
     4741 aattacagat gaaattgaaa aacctgaaga acagagcgca aggaatctgt gcggatttac
     4801 gggctgtttc tttcttatcg aaagccaggt tccagcggct gccattccca tgagagcaac
     4861 tgtaatgacc atgtaggcaa gatgatgcca gagcgtgaat gaaaatattc ttgtctgaag
     4921 tatctgaaga gcaattattc caaatgagat tagaaatatt ccagtaaaaa tcatatgatc
     4981 ctattctccg gttttaatct ttttcccttg aagaaacaat atagtgttcc ctgtgaaaag
     5041 agttgtctgt ctggacttcg acaagtattt taagttcttt tttaagttca ccgaattctt
     5101 cagagtgctt gtgctcaaga gcaaaagccg catcaggatg agctttaatg tttataaaac
     5161 ggcttccgta aagtttaact tctctctgta tgtccctgaa catctgaaaa accattgtgt
     5221 ctcttgatct tattataccg tttccgttac agtaaggaca aggttccgtc atggaggaga
     5281 tgttactttc agttgtcctt tttcttgtta tctgtatgag acccagacgg gtgaaataga
     5341 aaaccgaaca ttttgcaggg tctttttcca gttcccgttc aagctctgaa ataaccttct
     5401 ttttattgtt ctggtttctc atatctatga agtcgacaac gattatacct gctatatttc
     5461 tcaatctgag atgatatgct atctcctcaa ccgcttcaag attggttttg agaacagtgc
     5521 tttcgagatt tcccttgccg acaaattttc cagtattcac atcaatgacc gtcaacgctt
     5581 cggtctgctc aattataaga aaccctccgc ttttgagcca tatcttcttt ttcagaactg
     5641 aagttatttc cggctctatc gaataataat caaatatcgg atagggaaga tcgtagaatt
     5701 tgaccagctc atcaggattt tcgttgtaga actgaagata gctcctcaca ttcttttcat
     5761 cgagtctgct gtctgtaacg atctccttca gatcttcgat gccggtgttc tgtataattt
     5821 ctgtcaaagg atcaagtgaa tcatgaataa gtgccgcacc tttctctttt ttatatgcat
     5881 tctgtatctc aacccatttt tttatcagag agaccgcctc tttttcaatt attttcacat
     5941 cactgctgcc tgcaagagtt ctcaaaatta tgccgtaacc ctttttctga acttttgtgc
     6001 cgagctcttt caattcattt ctttttatat tatctgttat ctttctgctg atccctatgt
     6061 gcgatatggt cggcagaaga acgcaaaacc ttccgggcag cgatatgtgt gttgtcaaac
     6121 gggctccttt ctgacctatc ggctctttaa taacctgcac aagaacccgc tccccttcct
     6181 ttacatattt tgcaatgtcc ctttttacag gatgaccact ctgattttcc tgtgattcct
     6241 cgatctcgtc atcctgaacg gttttctgga actcttcata agtaatatcc gttctaagat
     6301 cgttgataaa aataaaaccg gtccttgtgc cgccgaattc aacgaaagcg gcctgcatcc
     6361 cgggaagaac cctttttacg atccctgagt acacatttcc gagacagtat cctgtatccg
     6421 acctcattat gtgaagttct acaagtttct tctcctcaat tattgcgacc cttttctcac
     6481 cgtgaacggc atttacaatt atctgtcttt tcataccggg atttccgcta agatacagta
     6541 agaggtcttt tctgtttcat cggtattatc ggcatgtctg cctgaacttt tttcaagtct
     6601 tcaattatct cattctcaag attcggttta agatggaaag tatgcacttc atttttcctt
     6661 ttaagtttca acagttcctt ttccaaaagt gccggggtaa gatgttttga tgcaaccgca
     6721 atattttcaa gccggtttgg aaaagatgtt tcccagcaga cggcttttac agtgggctca
     6781 tcgttgacat ggttcacaaa attgtcacag ataaaagtat ctcctgaata tgcaattgaa
     6841 cagtcgtttt ttctgataat aaaacctatt gactcaacta aatgatttac ctctatcgga
     6901 agaaattcga ttccgtcaag tgagaaaggt ttctcaaact ctaccggctg aaaatttatc
     6961 gttggtttgc tcttagaagg aagttttgta aaatccggcc agataagatc gttaagtatg
     7021 tgggtcttaa tctcagacat gattttttct gttgtgtaaa cagtaaaaac atggtttccc
     7081 agcgaaagca tgaaatcagc aaatattggg agattcttta tatggtcaaa atgagaatgt
     7141 gttataataa catttctgat ttcaagcagt tgaggtatat caagagaaga catcagtgag
     7201 ccggtgtcaa tgactgtaga aggggtgatc tgcatcgaga tacatttctt gttttcaagg
     7261 tcgctaccgt aacagccaag cggcagaatt gaaagcatgc ttacctcctt gttttggccg
     7321 gcgtatgcta tattctaaat agattcatgt caagaaaagc ttagttaatg caattaccga
     7381 aatatcacaa agttattttt tttacaaaaa tttgatattt tgtgtttttc tgctaaagtc
     7441 cattttgatg aaagttttgg ttactttaaa ataattcacc aatgtattga atgaggtatt
     7501 tgttatgttt aagaatttta catttttaat gcttattccg gcaattctga ttttgatctc
     7561 atgtaaccct gaagatcagt atctcagtaa gctttatccg aaggaatcaa gaaattatgc
     7621 gatccagtca ctgaaaaaaa tgaagggtga gatcggtaat cttgacaaaa catgcgaaaa
     7681 actgctcaaa cttgatagaa cacctgaagc cggagtagca gttcttgcag cgggtgagat
     7741 cgggtgcacg atgtcaattc cgaggattgg agaaattgtc gatgactgcc tcgtttcatt
     7801 aaatgtaaga aatcttaaaa ccctagaaag cgccgcaatg gcgatgggtc ttctcaaaaa
     7861 ccccgatgct gttccgatcc ttgcaaaata tttttccatc aacactcctg aagtgcttgc
     7921 agccggtatg cgtgaaaaag cagaatctgt cgcaaaaaga gcggcgatag aagcactttc
     7981 aaagatgccg aacgaaagca aacaccttgt tccggaaata ttgaaagttt ttaactcatc
     8041 cattgaagat ttcggaacaa aatacacaac tgccggaatt atgggtgaat ggcaggaccc
     8101 gtctactgtc cgtcctcttg tcacttctct tttttatgaa gaacagggat tcagtctttt
     8161 ccctgaagcc agaaaatctc ttatccgtct cggtaaactt gccgaagaag aacttataaa
     8221 ggcatacaac ggtcagaacc ctacagttaa tgacattcag aatgcaaaca aagacagagc
     8281 tacaaaacag ttctgccctg aatatattga caatgaagaa gcaaagaaaa agggtgattg
     8341 tccgaaagac gatgaattcg ttgccacaat agcttccata gaaaccacaa caaaacttaa
     8401 aacatccatt gtccttgcag atatccgttc aaaactcgct gttgacatgg ttattgcaga
     8461 acttgaagca cagcttgcaa ccgacaagaa acagccattc cttgcagaac atcttgctgt
     8521 tcagcttgca aaattcggag atttcaaggc aacagacact cttcttaaaa tggttagcaa
     8581 gaaattcaca aagaatgctg tgaaaaaacc tgaccacaaa ccctcaaaag aagagatcgc
     8641 tcaggcaaaa cttgctgaca ggggacagga aatatcaatc acgatgaaag gtgcagaagc
     8701 acttgcaata cttggtgatc cgaaatcaat tccatatctt cttgagcttg caaaacagaa
     8761 acctatctct gaatataacg agatgaatga aaagatactt ttctatgaat caatcgtatg
     8821 gggtgctgat gcattgacca gaatgatgta cgaaccggaa atggcatctg actttatcaa
     8881 acatgctgaa agtattataa gtgaaggcga aagctatgtc gcaggggtag aaaagaaaac
     8941 aaaagagcag atcttaaagg aaaccaaggg acaggaatta agtgctgaag accttgacaa
     9001 aaagatcaag acccaggcaa gacttgatgt taactatgat tcgacaaata agactgttga
     9061 aatgtttaag cggtttgtct caagggcgaa agtgtcacag gaatgcggca aagaggttaa
     9121 atgttacgtg gaaaagctta aggacaaaga ctctgccgta gttgaaaaag cggtttacat
     9181 gatcgggtat tcgggtgaaa taaaacatta taaagaccag ctcaaacctg ttttcacaca
     9241 tgttgagcca tatgtccgtg aagctctttc aatcgctctt cttaaaactg aagacaaagg
     9301 ttttgtgcca ataatcgaag aggttcttaa aaatgaagga gacaaggttg aatacgcact
     9361 tgcaatgaga gagtttagag ctatagcaag ttatctcagt tcacttaatt agatctgctc
     9421 ttgatcacct tctttattcc ttttgaggtt ctcacatgaa agttgcaatc gtcaatgtaa
     9481 accgttcaag aataagcaga aacacagagt accatctttt tacattaaaa gcttttattg
     9541 acgaaagagc cgtcagtgac attgaaactg agatcataaa catttatatg accgacgaca
     9601 tggcaactac cgccaacctt ttgaaaaata aaaatgccga cattattctt ttcagtgttc
     9661 tttactggaa ttcagcttac attttaaaac tcgctgaaag ctgcagaaca ttacctgcga
     9721 tcaaaggttt gtggggacac gacacattct cgcatccgga agaatacctc aagaaagatt
     9781 ttgattttat aattcaggat gaaccggaac tcgctctgta tgagatggca tcacttaaga
     9841 aagaaaacag agatatcagc tctgcaagcg gtataatatt caaagaccgg gtgaagaaaa
     9901 ccttcgtctt cggtgaaaac aaggtgatca gtgatcttga tcttattccg tccccatatc
     9961 tgaaggagat gatcgaagtt gatgagcaca cagtcgttta ctgggaagtc gcaaggggct
    10021 gtagttttaa atgcgatttc tgtgttgaat tttccaaggc aggcaatttg agatatcaca
    10081 gttttgctta tcttgaaaaa gaattgcgct attttgtatc cagaaatgtt tctcagatag
    10141 tgataggatg cccgatcttc aacttaagtc atcagcattt tgtcaaaata cttgatatgc
    10201 ttaaatttca tctgcctcat gcatctgttg agattcaggc aagaccggac cttctcacga
    10261 gagaggacat agaatctctg gctgaaatga atgtgtttat taacatcgga ttgcaaaccg
    10321 taaatcagaa agttcatgaa aacctgatga cttctcttaa tgttgaaaat gcacttaaca
    10381 tcatcaggta tatggcaaac tttcccaatc ttcctttcgg catagatata atcgccggac
    10441 ttccgaaaat gacatttgat gactttctta acgatcttga aaacgcattc aatctctggc
    10501 ctgtttcttt aaatgttttc aggttatcaa tgtatcctgg gacaagaatg ttcaacagaa
    10561 tgagagaata taattatacc gtagaacact cgtatccata cagagctctt gacaatccgc
    10621 aattcaataa aagagaattt gagaagatca gtgaaatggc tgaaggaata gatgtccttt
    10681 acaacaaagg cagaatggtg tctatcataa ctatgcttgc aaaaggactg gaaatgccat
    10741 gccatgagat cataacccgt tggaacaagt ggataaaaaa gcaacctgtt gacatgaatt
    10801 caatggaccc tgatgatatt aattacgatc agctttttga atatataaat gagtttttct
    10861 cctatctttt cgaccggttc caaaaaaaga agctgtggcc ggtcgcttca gatattctca
    10921 aacacaatta tttttacacc acatccttaa tgacacttga ggaggatatt ataacttatc
    10981 cttatcagat agaagccatt gataacaaaa ctttgatagg gatcaactgt tctgcctttt
    11041 ttgacaaatt ctcatacgac attgaagata ttatcgaaac cggctacatc gatttaaaga
    11101 aatattcctg cgatgttgac aaggaaagca tgtatggtct agtttaccgt cttgatggca
    11161 gtgttttcac aaagactatt actgatgaag aaggcagtat tttttccttc atacgcaaaa
    11221 agaattccgt ttcaatttct gaaattcaaa aaaagttcaa gaatatagat gttcttaatt
    11281 ttatcacttt ctggtgtgac gaaggcgttc tttttacagt tcagtaagct gacctcagaa
    11341 aaatcaaaaa aaaatggctc ctcgtgacag acttgaactg ccgacctagt ggttaacagc
    11401 cacccgctct accgactgag ctaacgagga accttaataa agatcacaga gccaacgaac
    11461 atcagcgaga ggtaatatac atatcatgag catggtgtca aatattttca caatcatttt
    11521 ttttattctg ataacattaa tgattacaag agattatttt ttgattaatt ttgcttgacg
    11581 gatacctttt ttcaagtaac attcttttgt ggattt
//
LOCUS       DAOOYB010000078        11593 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_5885_length_11593_cov_11.373635, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000078 DAOOYB010000000
VERSION     DAOOYB010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11593
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5885_length_11593_cov_11.373635"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            369..2948
                     /locus_tag="PL056_06145"
     CDS             369..2948
                     /locus_tag="PL056_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46630.1"
                     /translation="MLTRLSVLIFFLLVFSGCGQISTAGKEKENVSESFSDYQDDDNY
                     VSDLLNNDAAEGESSDFDFFDYDTEYADENINEKPDEDGVYEKYKFQWTFVKEEKDLA
                     HVFSRGAQNNAAIVFKNKFYILSGGNPSFILQSLNAVAWTVLSSENLPVFSGQGTVVV
                     HRDMIFALTESGLFVSEDGIKWDLVDLPGGIGLRTGWSLTSWNDHLWIAGGYAENITA
                     NDLWYSGDGKTWMIHSVFENVKVSDKQYHSWDAENGLVGMSFFKTDDYMFLFGGHLEK
                     KIDRYSIFPYGGGASFACSQRIFGYSDVSYRINKDSITETVEIVGDGLGITNAVAFRH
                     ADKNWITAGQIIKSDDYYEQRYTGGTTIAICSDNDPLSYFFMKKSKDLSNWDFDDTVL
                     PETVEKLPVILNYNDKVWIFGAENSSEIWNGTEQFFTYDNEKDYVCSRGEYKLGMSVV
                     IKNNEDVLKYKGFTKMPYLQITGEVDDLSPLFCLEEVESLEVQDTTMLKSLNGLTNII
                     RINHQMYFISTNLEKIEMINLGRIGEPAIISFNKLLKSIKINNLEKFPIELKISSNPM
                     LEETDVCSSESDLSLDGVTIIDTPNLRKNDFTEKITNALNIRIENVPISSVMFPKLEY
                     SEKIKLKEILEDKGDAVIFPKLKEAGDMTLVYLDPQVFPVLERINNDLEIYGNQLFKD
                     SNNFPALTSVGGRIEFINFFESEISGFNSLKTVGGISFSQGMVERVSGFDSLTTIDSF
                     SVSSNEFIKDFSGFIFLNRIQELSMKSIFIEGNKLLENIDALSIIRKISSIKILDNSS
                     LVDISALKNCTSIGDDFQIFSRGAGVLPVSQAKLITDHFISLGANEESIFFSGCFENS
                     SCE"
     gene            complement(2975..4423)
                     /locus_tag="PL056_06150"
     CDS             complement(2975..4423)
                     /locus_tag="PL056_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015379 - potassium:chloride symporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HPM46631.1"
                     /translation="MKYKLLIRPLAVIAAVTAFFMIIPAGIALYSNEIASAKAFFYVI
                     SFTIVIASVMIFVSRNWETGKMSTKEGFLLVFAGWIISSFVGALPFFISGAIPSFGDA
                     FFETASGFTTTGATILTSIESIPSSMLFWRSITHWLGGMGIVVLTVAILPVLGVGGLQ
                     LLKAEAPGPTVDKITPRIRETAKVLWLIYLGMTVAQTILLRFAGMSYFDALTHTFGTL
                     ATGGFSPKNDSVGAYNSAYIDGVITVFMILAGINFTIHYRLLIGKTKNILRNTELITY
                     LSILAVATMVMTINLYEKTYSTISESFRYASFQVASIMTTTGFATADFEKWPAFSQGI
                     LFLLMFFGACSGSTGGGIKVIRLVTLFKLALNEMKYLLHPRAVLPLWIDGKVIKKDMV
                     YSIAAFVFLYISMLLLTTLVCASSGIDLTTSFTASLATVGNIGPGFGSIGPTENYAFF
                     ADYVKWFLSFAMIVGRLEVYTVLIIFTKSFWK"
     gene            complement(4413..5765)
                     /gene="trkA"
                     /locus_tag="PL056_06155"
     CDS             complement(4413..5765)
                     /gene="trkA"
                     /locus_tag="PL056_06155"
                     /inference="COORDINATES: protein motif:HMM:NF007039.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HPM46632.1"
                     /translation="MKFIIAGAGSVGYDIARHLIKEGMDVVIIEKDTVRAKFLESDLD
                     CIVLNREINSIQTLKDADIQAGDYFIAVTESDEVNLIACSIASTFENVKKIARVRNLE
                     YSHAEVFGRPFLGADYVISPEIEAAKQITEIIDRGAATDVLTFEGTDITVRAGIIEKN
                     SIFKNKSLMKIRREINRQFIIALIKRGDKEIIPSGNTVLREGDTIFIVADSEAMNYIL
                     AKTGKRALKVKSALIVGTTRIARNLLRTLSVKPIDLKIVVENYDEAKELSRTFPNVLV
                     INGDVRNESLFEEEKLNEVDLIVTVTDNQELNVITALYAKSTGVDRAVAAVSKQSYMS
                     IAQKLGVDVTISPKKSAVDAILKYIRRGNIKTFHSLLEGDAEAIEFVVPEKCVAVGKL
                     IRELKMPFDSIIAAISRNGKVFIPDGSFQLQKEDSIIVILKSSVASKVETIFANTNEI
                     "
     gene            complement(5863..6894)
                     /locus_tag="PL056_06160"
     CDS             complement(5863..6894)
                     /locus_tag="PL056_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46633.1"
                     /translation="MRSLMGFYVVFSVVFFVSCANNELGKVDETDETNDESVDESVQT
                     EEETADTTDYELSDDLSDDLSGELSDEALAESETLSESEETTDEDEQPTEITEESFME
                     SVEGRYAHYDIVAYYSDMGLMGVFKNLIISYGFTTFEQEGGKLKITDRFCHSEQISNQ
                     DFTTTVPDALTQAIIPDSTFMEIKQDDNGGFYLFRPQTPTLLGIEYDDPYNTPLPESV
                     KPDDPRLVDADDDGNPGVTVFIDMFNKTEQLYIARREIFAFDAYLKENGNIEGIVYDE
                     SEQLIIDATSFLLKNQTGEWLQYRGENGDDYSLSPIILVPVDESYDCEKLMNERDSFF
                     PPNPAVWVD"
     gene            complement(7024..8133)
                     /locus_tag="PL056_06165"
     CDS             complement(7024..8133)
                     /locus_tag="PL056_06165"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPM46634.1"
                     /translation="MSIILALGAFPGIIFAVIKIFNKSEDISNRILGTAIFFYSTMLL
                     LVFLGKHSYISYSKEFIVTANTFISVSWPLFYLYIQLITGVTDRIAKKHLIHIIPFSV
                     YVAFFVIPALTISDLPDLFFRLSFIYSLFVSFGYSLLMFVNIRAYKIKFLEFSSEENT
                     AKVSWLKYSVLIWLFIEVLQFVFVPMRSYFLVCCPFVTVIHEFILNTASVSWIYLFAY
                     FAISHPDIFSQSKKMTESLKEEKLPSSTISDDYAEIIGKRIEKIMTEQKPYLNFDLTI
                     ATLGEMINVQPYLLGRYINNRLGKNFMTYINELRIQEVKSMFANPEFDDRTILEISLQ
                     SGFRTKSAFNSFFKRSTGATPLEYRKSIKQSRPHS"
     gene            8280..8618
                     /locus_tag="PL056_06170"
     CDS             8280..8618
                     /locus_tag="PL056_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6150 family protein"
                     /protein_id="HPM46635.1"
                     /translation="MKKVLFFTVLILLLTSTAYAQKVFSVQYENQADVKVFVVNYENQ
                     ADLKVYKVKYENQAGQNDGKWFFTQYANQAKKKIFFVKYENQADVKIYFVKYENQAGW
                     KNNSKKHFFF"
     gene            complement(8803..9132)
                     /locus_tag="PL056_06175"
     CDS             complement(8803..9132)
                     /locus_tag="PL056_06175"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HPM46636.1"
                     /translation="MKDEKIRNKIKAIVTEECNLRDFNVLKFILFGSRARQDNNKESD
                     WDFIVVLDKPVSWKDKMKLWLTINRRLAIIKADADIIFKSESEYEKDKKDVGKTTYYA
                     NKEGVLI"
     gene            9393..10217
                     /locus_tag="PL056_06180"
     CDS             9393..10217
                     /locus_tag="PL056_06180"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HPM46637.1"
                     /translation="MKKVLFLLMFAVIFMVVSCDLFEPGDKNISCMTENEERVVQCAK
                     DIEKEQKQICLDGEWTDKGFCYLCKEKEARTITCGTDIEKEQDQVCSNGEWVDKGFCY
                     LCEENEKRTIPCETNETMILNQTCILGDWYDDGKCRCSADDVFCHEKQGFWSDISNDT
                     MNWEDALVYCENLGGQPPSISELRELIIACSGTIFDGECEVTDDCLSYNDCWNSACKG
                     CENNNYLEYSIFDDIENLWSFSELSETTDYAWYVDFKNGSVNQSIKLNKHHVRCVK"
     gene            10509..11351
                     /locus_tag="PL056_06185"
     CDS             10509..11351
                     /locus_tag="PL056_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HPM46638.1"
                     /translation="MSGLVVFGDKKVRRVWHENEWYFSIVDVVGVLTDSVDASAYWRK
                     LKERLKKEGNEPVTNCHTLKMKAADSKMRETDVSNIEGLFRIIQSIPSPKAEPFKRWL
                     AKVGYERIEEIENPEIAAQRMREIYRQKGYSEGWIEKRMRGIAVRDELTGEWRKRGVK
                     ENLEFAILTAEISKATFGMTPAEYKKFKELSKPDENLRDHMTDLELIFTMLGEASTSE
                     IARTRDAQGFENNKEAAQVGGRIAGNARKELEKKTKAKVSTKDNFKRISELRKNELKN
                     KQEE"
     gene            complement(11352..>11593)
                     /locus_tag="PL056_06190"
     CDS             complement(11352..>11593)
                     /locus_tag="PL056_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46639.1"
                     /translation="IFRKEKPENIELEEGFELSQRKGYGDGNLVINGGFEFHLGKRVF
                     LMLEYIYRHPVVNDPGDYYSFLPNHFGGLAIRFER"
BASE COUNT         3671 a         2004 c         2367 g         3551 t
ORIGIN      
        1 gaaaacggga tgttgacggt gaaacaagag aagatctcaa ctactggata gaacagttcg
       61 aaaaagataa acatgaagcc gcacttaatt tctggtttga aataagaaaa ggaattgacg
      121 aatctttgag agatttccag taaatacagt cacttccaga acgattttgt gaatattata
      181 agcactgtat agacttcaag acggccgact atcattgcaa acgagagaaa ccattccttc
      241 caataggtag cttaactgtt tcctctattt ttttctattt tctaaagaaa tctgcggctt
      301 tacccttgac acaaaaatct taatgtaata tagtttttat gtactttaat tctctcagtg
      361 aggtcgcaat gttaaccaga ctgagcgttc taattttttt tcttttagtt ttttcaggtt
      421 gtggtcagat atctacagcc ggcaaagaaa aagaaaatgt ttctgaaagt ttttctgatt
      481 atcaggacga tgataattat gttagtgacc tcttaaataa tgatgccgcg gaaggtgaaa
      541 gttctgattt cgattttttt gattatgata cggaatatgc tgatgaaaat atcaacgaaa
      601 aacctgatga agacggggtt tatgaaaaat ataaatttca gtggacattt gttaaagaag
      661 aaaaagatct tgcgcacgtt ttttcccgcg gtgctcaaaa taatgccgcc atagttttta
      721 aaaataagtt ttacatttta agtggcggga atcccagttt tatacttcaa tctttaaacg
      781 cagttgcctg gacagttctc tcttctgaaa atctgcctgt tttttcagga cagggaacag
      841 ttgttgttca cagggatatg atattcgctc ttaccgaaag cggtcttttc gtcagtgaag
      901 acggtataaa atgggacttg gtggatctgc ccggaggaat cggcttaaga acgggctggt
      961 ctttaacatc atggaacgat catctatgga ttgcaggggg gtatgctgaa aatataactg
     1021 ccaatgatct ttggtactcc ggtgatggaa aaacatggat gattcacagt gtttttgaaa
     1081 atgttaaggt ttctgataag cagtatcatt cgtgggatgc ggaaaacggg cttgtcggga
     1141 tgtcattttt taaaacagat gactatatgt ttctgttcgg aggacatctg gaaaagaaaa
     1201 tagacaggta cagcattttc ccttacggcg gaggagcaag ttttgcctgt agccagagaa
     1261 ttttcggtta ttcagatgta agttacagga tcaacaaaga ttcaataaca gaaacagttg
     1321 aaattgttgg ggacggttta ggtataacaa atgctgttgc attcagacat gctgataaaa
     1381 actggataac agccggacag attataaaaa gtgatgatta ttatgaacag aggtatactg
     1441 gtggaacgac cattgccatt tgttcagata acgatccgct ttcatatttc tttatgaaaa
     1501 aaagcaaaga tctttcaaat tgggattttg atgataccgt gcttcctgaa actgttgaaa
     1561 aactacctgt aattctaaat tataatgata aagtgtggat tttcggtgca gaaaattcat
     1621 ctgagatatg gaatggaacg gaacagtttt tcacatatga caatgaaaaa gattatgtct
     1681 gttccagggg tgaatataaa ctcggcatga gcgttgttat taagaacaat gaagatgttt
     1741 tgaaatataa aggttttaca aagatgccat atttacagat tacaggtgaa gttgatgatc
     1801 tgagtccgct tttttgtctt gaagaggtgg aaagtctgga agtgcaggac acaacgatgc
     1861 ttaaatcgct gaatggctta acgaacataa tcagaataaa ccatcaaatg tatttcatat
     1921 ccactaattt ggaaaaaatt gaaatgatta atcttgggag aattggtgag ccggcaatca
     1981 tttcttttaa taagttgttg aaatctataa aaattaataa tttagaaaag tttcccatcg
     2041 aacttaagat aagcagtaat cccatgcttg aagagacaga tgtttgcagt tctgaatcgg
     2101 atttatcact ggatggagtt actattattg atacccctaa tcttagaaag aatgatttta
     2161 ctgaaaaaat tacaaatgct ttgaatataa ggattgaaaa tgttcctata agttcggtga
     2221 tgtttccaaa acttgagtat tctgaaaaaa taaaattaaa agagatttta gaggataaag
     2281 gtgatgcagt tatttttcca aaactcaaag aagcggggga catgacatta gtgtatctgg
     2341 atccccaggt gtttcctgtt cttgagagaa ttaataacga tctggaaata tacggaaacc
     2401 agttatttaa agacagcaat aattttccgg cacttacatc tgtcggagga agaatagagt
     2461 ttataaattt ttttgaaagt gaaataagcg gttttaattc attgaaaacg gtgggcggaa
     2521 tttcttttag tcaggggatg gttgaaagag tttcagggtt tgactctttg acaaccattg
     2581 attctttttc agtttcaagt aatgaattca taaaagattt ttcgggtttc atatttttaa
     2641 acaggattca ggaactgtca atgaaatcta tttttattga aggtaataaa ttgttggaaa
     2701 acatagatgc acttagtatt atcagaaaaa tcagttccat aaaaattctt gacaatagtt
     2761 ctcttgtgga catttccgcg ttaaaaaact gcacatcaat aggggatgat tttcaaatat
     2821 tttcccgcgg tgcgggcgta cttccagttt ctcaggcaaa gcttataact gatcatttta
     2881 tctcactggg agcaaatgaa gaatccatct ttttttcagg atgttttgag aattcatcgt
     2941 gtgaatagcc gccactattc tgattctttt tacatcactt ccagaacgat tttgtgaata
     3001 ttataagcac tgtatagact tcaagtcggc ctactatcat tgcaaacgag agaaaccatt
     3061 tcacataatc tgcaaaaaag gcataattct cggttggtcc gattgaaccg aatccgggtc
     3121 cgatatttcc cactgttgca agtgaagcgg tgaatgaagt tgtaaggtca attcctgatg
     3181 aagcacagac aagtgttgtc agaaggagca tggaaatata aagaaaaaca aatgccgcaa
     3241 tcgaataaac catatctttt tttattactt ttccatcaat ccacagagga agaaccgctc
     3301 ttggatggag aaggtatttc atttcgttga gtgcaagctt gaaaagtgtt acgagtctta
     3361 tcacctttat tccgccgccg gttgagccgg aacatgcacc gaaaaacata agaagaaaaa
     3421 gtatcccttg agaaaaagcg ggccattttt caaaatcagc tgttgcgaaa cctgttgtag
     3481 tcatgatcga agcgacctga aaagatgcat atctgaaaga ttctgaaatt gttgaatatg
     3541 ttttttcgta gaggtttatt gtcatgacca ttgtcgcaac tgcaagaatg gagagatatg
     3601 tgataagttc agtgtttctt aaaatgttct ttgtctttcc gatgagcagg cggtagtgga
     3661 ttgtaaagtt tattccggca agtatcatga aaaccgtaat aaccccgtca atgtaagctg
     3721 agttatatgc tccgaccgag tcattcttcg gggaaaatcc gcctgttgcg agagttccga
     3781 aagtgtgggt tagagcatca aaataactca tacccgcaaa tcttaacaga attgtctgtg
     3841 caactgtcat tccaagatag atcaaccaga gaacttttgc agtttcacgg atccttggag
     3901 taattttatc aactgtcgga ccgggtgctt cggctttaag gagctgaagc cctcccactc
     3961 caagaacagg aagtatggca acggtaagaa caacgatccc cataccgccg agccagtgag
     4021 taatgcttcg ccagaaaagc attgatgaag ggatggattc tatcgaagta agtatcgttg
     4081 cccctgttgt tgtgaatccc gaagcggttt caaaaaaagc atcaccaaaa cttggtatcg
     4141 ctcccgaaat aaagaaaggg agagctccta caaatgatga aattatccag ccggcaaaaa
     4201 cgagaagaaa tccttctttt gttgacatct tgccggtttc ccagttccgt gaaacaaaga
     4261 tcatgaccga agcgatcacg atcgtgaaag agataacata aaaaaacgct tttgctgatg
     4321 cgatctcatt tgaataaagt gcaataccgg caggaattat cataaaaaat gctgttactg
     4381 cggcaattac cgcaagcggt ctgatgagaa gtttatattt catttgtgtt tgcgaaaatg
     4441 gtttcaactt ttgaggcaac tgagcttttt aagatgacaa taatactgtc ttctttctgc
     4501 agctggaaac tgccgtcagg aataaaaacc ttaccgttcc tgcttattgc cgcaattatg
     4561 gaatcaaacg gcattttaag ttcccggata agttttccga ctgcaacaca tttttcggga
     4621 accacaaact cgatagcttc agcatcacct tcaaggagag aatgaaaagt tttgatgttg
     4681 ccccttctga tatattttaa aattgcatca actgcacttt ttttggggct tatggtcaca
     4741 tccacgccca gtttctgggc aattgacata taactctgct tgcttactgc cgccacggct
     4801 ctgtcaacgc ctgtgctttt tgcgtataaa gcggtgataa cattgagttc ctgattatcg
     4861 gtaactgtta ctataagatc tacttcattc agtttttcct cttcaaaaag tgattcgttc
     4921 ctgacatctc cgtttattac gagaacattg ggaaatgttc ttgaaagttc ctttgcttca
     4981 tcgtaatttt caacgacgat cttgaggtct ataggtttta ctgaaagagt ccggagaaga
     5041 tttctcgcga tccttgttgt tccgacgatc agtgcacttt taactttcag agcccttttc
     5101 cctgtttttg caaggatata gttcatcgct tcgctgtctg caacgatgaa gatggtgtca
     5161 ccctccctga gaacagtgtt gccagatgga attatttcct tgtcgcctct ttttataagt
     5221 gcaatgatga actgccggtt gatctctctt ctgatcttca tcagcgattt gttcttaaat
     5281 atcgaatttt tctcaataat tccggctctt actgttatat ctgtcccttc aaatgtaaga
     5341 acatctgttg cggcaccgcg gtcaattatc tcagttatct gtttagccgc ctctatttca
     5401 ggggaaatga cataatcggc accgaggaac ggtcttccga aaacttcagc gtgcgaatac
     5461 tccaggtttc tgactctggc gatctttttt acattttcaa aagttgatgc gatcgagcag
     5521 gcaatgagat tgacttcatc actttcagtt acagcgatga aataatcacc cgcctgaatg
     5581 tcggcatctt ttaatgtctg gatagagttt atttcgcggt tgagtacgat gcagtcgagg
     5641 tcgctttcca gaaactttgc tctgacagtg tccttttcaa ttattacgac atccattcct
     5701 tcttttatga gatgtcttgc aatgtcataa ccgactgacc ctgcacccgc aataataaat
     5761 ttcattgaat taccttaaaa acagtaaatg aacattcccg ccaatattaa agaacttatt
     5821 tttattgtca atttatagga aaaggagggg agtttttaag attcagtcta cccagacagc
     5881 gggattgggg gggaaaaagg agtctctttc gttcatcagt ttttcgcagt cataactctc
     5941 atcaacagga acaagaatga tcggacttag tgaatagtca tctccgtttt cacctctgta
     6001 ctgaagccat tcgccagtct gattttttaa cagaaagctt gtggcatcaa ttataagctg
     6061 ttccgattca tcataaacta tcccttcgat atttccgttc tctttcaaat atgcatcaaa
     6121 agcgaagatc tctcttcttg caatgtataa ctgctcagtt ttattaaaca tgtcaatgaa
     6181 aaccgttacg ccgggatttc cgtcatcatc agcatcaaca agccgtggat catccggttt
     6241 gaccgattca ggaagcggtg tgttgtaagg atcgtcatat tcaattccaa gaagtgtcgg
     6301 agtttggggt ctgaaaagat aaaaacctcc gttatcatcc tgttttattt ccatgaaggt
     6361 tgaatccgga attattgcct gcgtcagtgc atcaggaaca gttgtcgtga aatcctgatt
     6421 tgagatctgt tcggaatggc agaagcggtc tgttatcttc aattttccgc cttcttgttc
     6481 aaaagtagta aaaccgtatg aaattataag gtttttgaac actcccatca atcccatgtc
     6541 tgaataatat gcaacaatgt cgtaatgggc ataccgtcct tcaacgcttt ccataaagct
     6601 ttcttcagtt atttcagttg gttgttcatc ttcatcagtt gtttcttccg actccgacaa
     6661 ggtttccgac tccgccaagg cttcgtcgga tagctcgccg gacaagtcat cggacagatc
     6721 gtctgaaagt tcataatcag ttgtatcggc agtttcctct tcggtctgta cactttcgtc
     6781 aacagattca tcatttgtct catctgtttc atcaactttg cctaattcat tgttggcaca
     6841 ggatacgaaa aaaacaacac tgaaaacaac ataaaatccc atcaatgatc tcattgcgac
     6901 ctccaaaatg ataaaaaaaa caccaacacc ggatgctttt ttaccaaagc tgtgaaatga
     6961 attcgtcctt gattctgaat ttttgcgaaa aagtactttt ttaaggcgga aaactgtttg
     7021 aatttatgaa tgcggacggc tctgttttat agattttcta tattcaagag gagttgcacc
     7081 ggttgatctt ttaaagaaac tgttaaaagc cgattttgtc ctgaatcccg attgaagtga
     7141 tatttcaagg attgttctgt catcaaattc cgggtttgca aacattgact taacttcctg
     7201 aattctcagc tcatttatat aggtcatgaa gttcttgccg agccggttgt ttatatacct
     7261 tccaagaaga tatggctgga catttatcat ttcgccaagc gttgcaatcg tcagatcaaa
     7321 attgagataa ggtttctgtt cagtcataat tttttcaatt cttttcccga ttatttccgc
     7381 ataatcatca ctgatagttg aagatggcag tttttcctct tttaaggatt cagtcatctt
     7441 ttttgactgt gaaaatatgt cagggtggga gattgcgaaa taggcaaaaa gatatatcca
     7501 cgaaactgaa gcagtgttca ggataaattc gtggataact gtgacaaaag gacagcagac
     7561 aagaaaataa cttctcatcg ggacaaagac aaactgaaga acttcaatga aaagccagat
     7621 cagaactgag tatttcagcc atgagacttt cgcagtattt tcctcagagc tgaattcaag
     7681 gaatttgatc ttgtaagccc ttatatttac aaacattaac agagaatatc caaatgaaac
     7741 aaaaagagaa tagataaaag aaagtctgaa aaaaagatcg ggcaggtcgc tgatggttaa
     7801 agcaggaatg acaaaaaaag caacatacac cgaaaaaggg atgatgtgaa tgagatgttt
     7861 ttttgcaatt cggtcagtaa cacctgtaat taactgaata tataagtaaa acagcggcca
     7921 cgaaacgctg ataaaagtgt ttgctgtgac aatgaactct ttcgagtaac ttatataact
     7981 gtgctttccg agaaatacaa gaaggagcat cgttgaatag aaaaatatcg cagttccaag
     8041 gattctgtta cttatatctt cggatttatt gaatattttt attaccgcga aaatgatccc
     8101 cggaaatgct ccaagtgcaa ggattatgct cattaaagat cctgaaaaaa gttcaaaaag
     8161 ctcaaaatcc taaatggaat ggactttcaa gtcaattttt tgaactgttg ccgaaaaatt
     8221 gtttttattt gtgtaagata tatcattttc tcaaagatct ttatatttgg agggtcaata
     8281 tgaaaaaagt tctatttttt acagttttga ttcttctgct cacatcaaca gcttatgcgc
     8341 agaaggtttt ttcagttcag tatgaaaatc aggcagatgt aaaggttttt gtagtaaatt
     8401 acgaaaatca ggcagatctt aaagtttaca aggtgaaata cgaaaatcag gcagggcaga
     8461 atgacggaaa atggtttttc acccagtacg ccaatcaggc aaaaaagaag atcttcttcg
     8521 taaaatatga aaatcaggcc gatgtaaaga tctattttgt aaagtacgaa aatcaggccg
     8581 gctggaaaaa caattcaaaa aaacacttct ttttttaaaa cctgaaccta cctctcaaat
     8641 ctgatagcga gtccgccgaa gtggttgggc agaaatgaat agtaatcgcc ggggtcgttc
     8701 acgaccgggt ggcggcagat attttctttt gaataaagtt tttaacagtt tcagcaagct
     8761 taattgcata tccagcttct tcgttggtgg gaatattatt gctcatatca gaacaccttc
     8821 tttatttgcg taataagttg tttttccgac atcttttttg tctttttcat attcagactc
     8881 gctcttgaaa ataatatccg catctgcttt tataatcgca agtctgcggt taattgtaag
     8941 ccatagtttc attttatctt tccagctgac cggtttatcc agaacgacaa taaaatccca
     9001 atcactttct ttgttgttat cttgtcttgc acgacttcca aaaagaatga attttaaaac
     9061 attgaaatct cgcaagttgc actcttcagt gacaattgct ttgattttgt ttctgatctt
     9121 ttcatctttc atttaagccc cctttaaaaa ccgtctgata agtgtatctt aaaaaataaa
     9181 catttcaagg gaaaaaacct ctccaacatg tgtaaccatc ttaaaagctt ctgatttttt
     9241 ttctgtatca ttcatctgat tcaaaaacac tgtcatttta tcctttcttg atttatgagg
     9301 atatgagtcc gccgagaaaa agttccaaaa aattgtgttt atttatgtaa gatatatcat
     9361 tttctcaaga ttttgcgaag tggagggtca atatgaaaaa agttttattt ttattaatgt
     9421 ttgctgtgat ttttatggtt gtttcgtgtg atctgtttga gccgggagat aaaaatataa
     9481 gttgtatgac cgaaaatgaa gaaagggttg ttcaatgtgc aaaagatatc gagaaagaac
     9541 aaaaacagat atgcttagat ggtgaatgga ctgataaagg attttgctat ctttgtaaag
     9601 aaaaagaagc aagaacaatt acttgcggaa cagatattga aaaagaacaa gaccaggtat
     9661 gttcaaacgg tgagtgggtg gataaaggat tttgctatct ttgtgaagaa aacgaaaaaa
     9721 gaacaattcc atgtgaaaca aatgaaacaa tgatactgaa ccaaacttgt attttgggtg
     9781 actggtacga tgatggtaaa tgccgatgtt cagcagacga tgttttctgt cacgaaaaac
     9841 aaggattctg gtcagatatt tcaaatgata ctatgaattg ggaagatgcg ttagtttatt
     9901 gtgaaaactt aggaggacaa cctccttcaa tttcagaact aagggaactc attattgcct
     9961 gttcaggaac gatatttgat ggagaatgtg aagttacgga tgattgcctt tcgtataatg
    10021 attgttggaa cagtgcgtgt aaagggtgtg aaaacaacaa ctatctggaa tattccattt
    10081 ttgatgatat agaaaatctt tggtcatttt ctgaactgtc agagaccaca gactatgcat
    10141 ggtatgtgga tttcaaaaat ggaagtgtga accaaagtat caagttgaac aagcaccatg
    10201 tccgctgtgt aaagtagaaa acaagtctgt cggtgataag caaacaacaa taaaaaatgc
    10261 gtataaagat ataattttat ttagattttc cctgcctctc aaatctgatg gcgagtccgc
    10321 cgaaatgatt tggcagaaat gaatagtaat cgccggggtc gttcaaaact gggcttttga
    10381 atttgattaa ctgtaaatat tgtaaaaagt gttttaagtt aaaataaaat tgtatttaaa
    10441 atcaaaatat tatatcttgg caaagtttaa gcaattcttt gttaacatgt ttgaggagag
    10501 gtgttaagat gagcggtctt gtagttttcg gtgacaaaaa ggtaagaagg gtttggcatg
    10561 agaatgagtg gtatttctca attgtggatg ttgtcggtgt tttgacggat agcgttgatg
    10621 caagtgctta ttggagaaaa ttaaaagaac ggcttaaaaa agaaggaaat gaacccgtga
    10681 caaattgtca cactttgaaa atgaaggcag cggacagcaa aatgagagaa accgatgttt
    10741 ccaacataga aggtcttttt cgtataattc aatcaattcc atcccctaaa gctgagccat
    10801 ttaaaagatg gcttgcaaaa gtcggatatg aaagaattga ggaaatagaa aatcctgaaa
    10861 ttgctgctca aagaatgcgt gaaatatata gacagaaagg atattcagaa ggttggatag
    10921 aaaagagaat gagaggaata gctgtgagag atgagctgac aggcgagtgg agaaaaagag
    10981 gtgttaaaga aaaccttgaa tttgcgatac tcacagctga aatcagcaag gcaacttttg
    11041 gaatgacgcc tgctgaatat aaaaaattta aagaactttc caaacctgat gaaaatcttc
    11101 gtgatcacat gacagatctt gaacttattt tcactatgct gggagaagct tccacatctg
    11161 aaattgcacg gacaagagat gcacaagggt tcgaaaataa caaagaagca gcacaggtcg
    11221 gtggtagaat tgctggcaat gcaagaaaag aattagaaaa gaaaaccaaa gcaaaagtgt
    11281 caacgaaaga taactttaaa agaatttctg agttaagaaa gaatgagttg aaaaacaaac
    11341 aggaagagtg actacctctc aaacctgatg gcaagtccgc cgaagtggtt gggcagaaat
    11401 gaatagtaat cgccggggtc gttcacgact gggtgtcggt agatatattc aagcatgaga
    11461 aaaactcttt tgccgaggtg aaattcgaat ccgccgttga tgacgagatt tccgtcgccg
    11521 tatccttttc tttgagataa ttcaaagccc tcttcaagtt caatgttttc aggtttttct
    11581 ttgcggaata tcc
//
LOCUS       DAOOYB010000079        11493 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_5962_length_11493_cov_11.036020, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000079 DAOOYB010000000
VERSION     DAOOYB010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11493
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5962_length_11493_cov_11.036020"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..938
                     /locus_tag="PL056_06195"
     CDS             129..938
                     /locus_tag="PL056_06195"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HPM46640.1"
                     /translation="MSLKHRWMKLRNFQSADFWAMVFARPLTILFLFPVIEKKWVTPN
                     RVTFLSVITKIAGAGFIAIDMSYRGGVIGAVLVNLGLILDNMDGTIARYHNTASKFGF
                     FYDKATDAVTMTIMFWAMGYRGYLMTGDMSDLIIPLLAATCVYITGYSKWVAERVLFD
                     MKISEKFHKSQLNEYAADQEQGYIWTPPPQRSFTDWLKWLGKAFFSILYFNEVDIFFW
                     AGLALITEEMWIFTKFDSGFMLLGLFVGPFLFGYKVRKKERELIKLKKGLN"
     gene            938..2248
                     /locus_tag="PL056_06200"
     CDS             938..2248
                     /locus_tag="PL056_06200"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF004490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HPM46641.1"
                     /translation="MRLFYDIIADKRDKRELTGEDISYFLESYLHGELSDYQMSAMLM
                     AIFFNGLSENELKTWVEKMLFSGEVLDFSHIDGPVVDKHSTGGVGDKISIPLAPLLAE
                     LGFCVPMISGRGLGHTGGTLDKLESIPGFSVNIEKDRFNEIINKNRMLLIGQTPQIAP
                     LDKKLYALRDVTGTVESIPLIASSIMSKKLAEGINGLIIDLKIGGGAFMKTMESGRKL
                     AETMKDIGKRFNTHVDVLFTDMNEPLGLTTGNSLEIIESVEVMKGKYVPQVTELVLRM
                     AATLMMTFKMCRSIEEGEKIALEKLQSGTVAERFRKTVELQGGDSRVIDEPEKILPSS
                     KFRKEIFADQPGFIQKMDALSFGKALVQLGGGRLRKEDGINYGAGFVFNKKVKDAVKT
                     GDLLYTVHYDDKDSILKAEEYLKSAVVTTGHKPPSESSIILGSM"
     gene            2257..4518
                     /locus_tag="PL056_06205"
     CDS             2257..4518
                     /locus_tag="PL056_06205"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HPM46642.1"
                     /translation="MNINRFFFLRKFSPAFSNYLHKYIFRIIFKKADPEIPDLDLLKE
                     TPPDKKIVTVYISGRKSRIEQFLLCGFMIDNKITVPTHSFGPKTIFFRKHRELWIMLL
                     SLIKKTMLKEMQIESLYIPVDSPEELAIDPVFIKIFHKIKFKEARVIPVITLWNKDLD
                     RKSRSEWIEKYIGKYNLWSTSRELLLLLIKRRKLTLRIEHPVVTPIDSTPEVFIRKLY
                     KRMEDSKLNVVGASLKNWLDLKNDALLDLDLETDGERKQALKIMDSMAGKYSPVLAEI
                     YTSATGRLFKTVFSRFHYSDDEIKLLRRLCAMQGANMILVPTHKSYFDYLILNYVLYK
                     EKVTVPLVASGDNLDFFPLSMILRKMGAFFIKRTMKDDNLYRDVLKSYLKQVISAGYN
                     IEFFIEGGRSRSGMVRSPRMGMLKMLSETAKSTSRKLYVVPVSITYEKLKEIEEYKKE
                     RTGEKTPESDNFFQRLRNLFKVNYGPVYIRFARPVYLGSKFSAETAYRIAEIQEKSSV
                     ISFSSIFSTLFLSFSELSSSQIVERMEFINSELQKLSYIQTSMSLDNLDVNCSKLLRK
                     LVKKGDIRLVPPRKEFFSLSPEAVSEFSFYKNSVAFAFAPFFCLLFEGSQTHPFISEY
                     LEIAIMGYNRQLKPETAVNTADYPEWFVILLKKFFFEKFVLLKILIEAMKNRSDLFGK
                     TVTHTELVEKLFPHLTAEIQNVSKDQLFELIFFLEKKSVLTNNLTDFNSDLADKFISE
                     ADGVVKLLTEEKS"
     gene            4515..6275
                     /locus_tag="PL056_06210"
     CDS             4515..6275
                     /locus_tag="PL056_06210"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HPM46643.1"
                     /translation="MIELVYIPSFIKITSISGKNAVCYGSEKELDEKIKAADSEYFLI
                     IYESDYKPDSIAVKNSYATIIISFGCDFEQIDHFTFRVDGEKITRFSLECIISLVETT
                     SSLYDRIIENLQIGISLTVEKEPDNLYSSILTFLRKATGAEAGTLYLMSRERDKIFFI
                     SSQNSRFDSKMIEKKEIPFNRQSLVGYVCHNGEPLNIPDAYNIPESAPYQFNKEIDKK
                     LNYKTVSIITVPMKTTTGEIVGAVQLINKIDERSKTEIPFSDFDEMMLMSLSTLAAVS
                     VENNNLLLETETLLNNMIISSVKAIEQRDPATKGHSLRVAAYTLALLKRVASRPDILK
                     NETVDRYTVKTAETAALLHDFGKVGVREKILMKQGRIYSEQVEAVKWRMRFIIASVNG
                     REEDIAIVREMVEKIDGLNIPRPHTDEEKQLIEKAGGLTFEVDGMTVPVLTAEELDFL
                     KIKSGTFNDSERADMEKHVIYSHELLNAIDWPQHLKNVPEIASSHHEKLDGSGYPFQK
                     SAKDLGIIERILGIVDIYDALTAKDRPYKKAIPPEIAISIIEKEVAAGKLDPEIFSIL
                     VREREEIEQEVSRKIKENKL"
     gene            6301..7329
                     /locus_tag="PL056_06215"
     CDS             6301..7329
                     /locus_tag="PL056_06215"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HPM46644.1"
                     /translation="MKNIVLVIMAAFSLFLTISCEQEKEENIYGEYEDKNYEQPDENF
                     LPDEFQKKPDEDSIVINDSEPDDQSDSESESTDEEQVDPYKDMVLVPQGYAWMGCNEN
                     VETDCPAAELPYLEISTKAYYIDKYEVTVSDFQKCIDAKACGDEEFAYDTVSGNPYCN
                     LGARDTHPMNCVSYFGAQAYCLWVGKRLPSEGEWEKAARGGCEIHGDDNCEEKSYIYT
                     WGNEGEPSCANVSMAVMDGPNIDYGCGSDSTSVGGSYEAGKSPYGAYDMLGNLFEWVD
                     DEWSADHSTAKPDGSSTGSADGEAVIKGGGFMQTNKAEFRISYRAGAYVDEILFSRGF
                     RCVADIEN"
     gene            7574..9481
                     /locus_tag="PL056_06220"
     CDS             7574..9481
                     /locus_tag="PL056_06220"
                     /inference="COORDINATES: protein motif:HMM:NF026177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HPM46645.1"
                     /translation="MKLLTKFVLATVSIFIILAFAISYVSIKWINSNTINDAQHRVEL
                     TMKSARDIFNKKNDSTLQLLEVFAQNEVLRDYLKNPSDKELSEKVMERFRNLRISYNM
                     DILNIISPDGTVMMRTRPPFNKGDDVSDDPIVKNVLKKKKSVKGVYVLTKSLLMDEGT
                     SLYQRTIDTTGESDAMIMGTAIPVMHGEKMIGIISAGFLLNGNNDLVDEIRDSILYGD
                     YYKGRSVNTVTIFMGDKRIATNVVGENAGRATGTRASEKIVSTVMQNGDPWTGRAWVV
                     DAWYLSRYESIRNPDGGIIGMLYVGELEEKYRDIRFKAVTSILSVILAAMVFSILVFT
                     FIAWGILVPVKNLHNATLMLMNGDRKARIKGNYSGEIGHLTNSFNNMANDIEKHTLEI
                     EKKQKELAALNNELELTNRNYIDMLGFVSHELKNPLASAVMGLHTVKDGYLGELNEKQ
                     LKALNSVAESLDYFKDLIKNYLDLSRLEKGEYIVQRQNIFLKNAVIDPVLQQFTEEFK
                     SRHISIDLGVHDDTKVDADPDLLRIVYDNLISNAVKYGKEGGVIRLREKIVDGRFVFS
                     VYNEGVGIPKEKMTLLFKKFSRIYRAEHAGKRGTGLGLYSCHEIIKKHGGKIWAESVP
                     DKWVEFSFEIG"
     gene            9493..9891
                     /locus_tag="PL056_06225"
     CDS             9493..9891
                     /locus_tag="PL056_06225"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPM46646.1"
                     /translation="MVNKILIADDDVQMVNAVKTLLENVGYDTVSTNDPKEVIELARK
                     EMPDLIMLDVMFEGMTGPNGFELSRKIASDKDLKKIPVIILSGVKKVISSDFEVTPDK
                     EWLPVAAFLDKPVKPDQLLKEINNLIQKRS"
     gene            9894..10364
                     /gene="nuoE"
                     /locus_tag="PL056_06230"
     CDS             9894..10364
                     /gene="nuoE"
                     /locus_tag="PL056_06230"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005722.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HPM46647.1"
                     /translation="MESQVKKVLEKYPGAGRDKLIPILQEVQGLDGFISKNSVIEIGK
                     HLNLAPSKIFGVATFYNQFRFEPKGKHHILVCRGTACHVKGSAETLDAIERELGIKAG
                     NTTKDGLFSIEVVACLGACGLAPLICVDNEFYANATRERVKEIIKSYRAKEREA"
     gene            10366..>11493
                     /locus_tag="PL056_06235"
     CDS             10366..>11493
                     /locus_tag="PL056_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit F"
                     /protein_id="HPM46648.1"
                     /translation="MKNNIANNCWSDAGTASDALLEKLVKKGFTLKPGNPDEIKKLTR
                     ETIDKTVIYIGAGTCGLGAGAAATRKEIEKYIADKSIDARIIEVGCIGLCSAEPLVDI
                     QIPGRTRVSFRNITSEKVEKLFDDFFNGVVDEDKVLGQFHCQSSLKWDNIPFLSQHPF
                     FAPQKRWVLINCGIVDPLSIEEYVSRGGYASLNDALAKTPGDVCNEVEKSGLRGRGGG
                     GFPTGKKWKFAAAEKSEQKYLICNADEGDPGAFMDRAVIEGDPFRLLEGMTIAAYGIG
                     ASKGYIYIRAEYPLAIERLYFAIDKAKKYGLLGSNILGTGFNFDLQIKMGAGAFVCGE
                     ETALINSIEGKRGMPRPRPPYPAVSGLFGKPTVINNVETLAN"
BASE COUNT         3725 a         2262 c         2522 g         2984 t
ORIGIN      
        1 ctccataaaa aaaccggact attatatccg taatcttata taattcccac ttttttgact
       61 ttaaagatat cgggtctaaa ataaataagt ttttttattt tttattgtaa ttagttttgg
      121 agatactgat gtcactaaag cataggtgga tgaaacttag aaactttcaa agtgccgatt
      181 tctgggcgat ggtgtttgca agaccgctaa cgatcctctt tctgttcccg gtgatcgaaa
      241 aaaaatgggt aactcccaac agagtcacat ttttaagtgt cattactaaa attgcgggag
      301 caggtttcat agcaattgac atgagctacc gcggaggggt cattggagca gtgcttgtaa
      361 atctcggtct cattcttgat aatatggacg gaacgatagc aagataccac aacaccgcat
      421 caaaattcgg atttttctac gacaaggcaa ccgatgcagt taccatgacg atcatgttct
      481 gggccatggg ataccgcgga tacttaatga caggcgatat gtcagacctg ataatcccgc
      541 ttcttgcagc aacatgcgtc tacatcacag gatattcgaa atgggttgcg gaaagagttc
      601 tttttgatat gaaaatatct gagaaatttc acaagtccca gcttaacgaa tatgcggcag
      661 accaggaaca gggctatatc tggacacctc cccctcagag atctttcact gactggctga
      721 aatggcttgg aaaagcattc ttctcgatcc tttatttcaa cgaagtcgac atattcttct
      781 gggctggact tgcacttata acagaggaga tgtggatatt taccaagttc gactcgggct
      841 ttatgctcct tggacttttt gtgggaccgt ttcttttcgg atacaaggtc agaaaaaagg
      901 agcgtgagct tataaaactt aaaaaaggac tcaactgatg cgtcttttct atgacatcat
      961 tgcagataaa agagacaaaa gagagttgac aggtgaagat atttcatact tccttgaaag
     1021 ttacctccat ggagaattaa gcgattatca gatgtctgca atgcttatgg cgatattttt
     1081 caacggactg agcgaaaacg agcttaaaac atgggttgag aaaatgcttt tcagcggtga
     1141 agttctcgat ttttcacata ttgacggacc tgttgttgat aaacactcga ccgggggagt
     1201 cggagataaa atatcaattc cgcttgctcc gttattggcc gaactcggat tctgcgttcc
     1261 gatgatatcg ggcagagggc ttgggcatac tggtggaacc cttgataaac ttgaatcaat
     1321 tcccggattt tcagtgaaca tagaaaaaga ccggtttaac gaaataatca ataaaaacag
     1381 aatgttgctt atcggtcaga ctccgcagat agcacctctt gacaaaaagc tctacgcttt
     1441 acgggatgta acgggaacag ttgaatcgat ccctcttatc gcttcctcaa taatgagcaa
     1501 aaaactggct gaggggataa acggactgat aattgatctg aaaataggcg gcggcgcctt
     1561 catgaagacc atggaatcag gcagaaaact cgctgaaaca atgaaagata tcggaaaacg
     1621 atttaatacg catgttgatg tcctttttac agatatgaat gaaccgctcg ggcttacaac
     1681 aggcaactct cttgaaataa ttgaatcagt tgaagtgatg aaggggaaat atgttccgca
     1741 ggttacagaa cttgttctga gaatggctgc aactttgatg atgactttta aaatgtgccg
     1801 ttccatcgaa gaaggcgaaa agatcgcgct tgaaaaactt caaagcggta cagttgccga
     1861 aagattcaga aaaactgttg aacttcaggg cggtgacagt cgtgtcatag acgaacctga
     1921 gaaaatactc ccctcttcaa aattcaggaa agagatcttt gcagatcaac ccggattcat
     1981 tcagaaaatg gatgctctgt cttttggaaa agcacttgtt cagcttggcg gaggacggct
     2041 tagaaaagaa gacggcatta actacggcgc aggatttgtt tttaataaaa aagtcaaaga
     2101 tgctgtcaaa acaggagacc ttctctacac agttcattac gatgacaaag acagcatcct
     2161 gaaagcagag gaatatctta aaagtgcagt tgtgacaaca ggtcacaaac ctccttcgga
     2221 aagcagcatt attcttggct caatgtagag aaatggatga atataaacag attctttttc
     2281 cttagaaagt tctctccggc attttcaaac tatcttcaca aatacatttt caggataatt
     2341 ttcaaaaagg ccgacccgga gatccccgac cttgatcttc taaaagagac gcccccggat
     2401 aaaaagatcg taactgtcta tatttcagga agaaagagcc ggatcgaaca gtttctcctg
     2461 tgcggtttca tgatagataa caaaatcacc gttccgacac actctttcgg acccaaaacg
     2521 atctttttcc gaaaacaccg ggaactgtgg ataatgctcc tttcacttat aaagaagacc
     2581 atgctgaaag aaatgcagat cgagtctctt tacatacctg tcgactctcc ggaagaactc
     2641 gcgatagatc ctgtattcat aaaaatattt cataaaataa agtttaaaga agcaagagtc
     2701 attcctgtaa taacgctctg gaacaaagat cttgaccgga aaagccgatc tgaatggatt
     2761 gaaaaatata tcggaaaata caatttgtgg tcgacatcaa gggaactgct cctgctcctg
     2821 ataaaaagga ggaaactcac tttaaggatc gaacatcctg ttgttactcc gatagacagc
     2881 acacctgaag tattcataag gaaactttat aaaagaatgg aggactccaa gctgaatgtc
     2941 gtcggtgcgt ctcttaaaaa ctggctcgac ctgaaaaacg atgcgctgct cgatcttgat
     3001 ctggaaacag acggagagcg gaaacaagct ctgaaaataa tggactcgat ggccggaaaa
     3061 tacagccctg tccttgcaga aatatatact tcagcgaccg ggcggctttt caaaactgtt
     3121 ttttcaagat ttcactactc tgacgatgag atcaagcttt taagaagact ctgcgcaatg
     3181 cagggagcca acatgatcct tgttccgaca cataaaagct attttgatta tctgatactc
     3241 aactatgttc tttataaaga gaaagttact gtccctcttg tggcatcagg cgacaatctc
     3301 gatttttttc ctctttcaat gatcctgcgg aaaatgggag cttttttcat taaaagaaca
     3361 atgaaagacg acaatttata cagggatgtc ttgaaaagtt atctcaaaca ggtcatttcc
     3421 gcaggttaca atatcgagtt tttcattgaa gggggaagat cacgaagcgg aatggtccgt
     3481 tctccgagaa tgggaatgct gaaaatgctt tcggaaacgg caaaatccac atcaagaaaa
     3541 ctttatgtcg ttcctgtttc cataacatac gaaaaattaa aagagatcga ggaatacaaa
     3601 aaagagcgta caggtgagaa aacccctgaa tcagacaatt tctttcaacg gctgagaaat
     3661 ctttttaaag tcaactacgg tccggtatac atccgttttg cccgtccggt ctacctcggc
     3721 agcaaattct ccgcagaaac tgcataccgt attgctgaaa ttcaggaaaa atcgtcagta
     3781 ataagtttct cttccatctt ctcaacgctc tttttaagtt tcagcgagct ttcatcaagt
     3841 cagatagttg aaagaatgga attcataaac agcgaactgc aaaagctctc atacatacag
     3901 acatcaatgt cgcttgacaa tcttgatgta aactgttcaa aacttttaag aaaacttgta
     3961 aagaaaggcg acatacggct tgtccctccc agaaaagagt ttttttcgct ttcaccggaa
     4021 gcggtttctg aattctcctt ctataaaaac tcagtggctt ttgctttcgc cccttttttc
     4081 tgtctccttt ttgaaggttc gcaaacccat cctttcataa gcgaatatct cgaaattgcg
     4141 ataatgggat acaaccgtca gttgaaacct gaaacagcag taaatacagc agattatcct
     4201 gagtggtttg tgattcttct gaaaaaattc ttttttgaaa aatttgttct ccttaaaata
     4261 cttattgaag cgatgaaaaa caggtcggat ctttttggta aaacagtaac tcacactgaa
     4321 cttgtcgaaa aactttttcc ccatcttaca gcagaaattc aaaatgtttc aaaagatcaa
     4381 ctgtttgaac tgatcttttt tcttgagaaa aaatctgttt tgacgaataa tctgactgat
     4441 ttcaatagtg accttgctga taaatttatt tctgaagcgg acggagtcgt aaaactgctc
     4501 acggaggaaa agagttgata gaacttgtct acatcccttc atttataaaa ataacatcaa
     4561 tatcaggcaa aaacgcagtc tgttacggat ctgaaaaaga acttgatgaa aaaataaaag
     4621 cggctgattc agagtatttt cttataattt atgaatcgga ttacaaacct gattcaattg
     4681 cagtaaaaaa cagctatgcg acaataatca tttcctttgg atgtgatttt gaacagatag
     4741 accatttcac attcagggtt gacggagaaa agatcacccg gttttcactt gaatgcataa
     4801 tctctcttgt tgaaaccact tcatctcttt atgacagaat aattgaaaac cttcagatcg
     4861 gcataagtct taccgttgaa aaggaaccgg ataatcttta cagcagtatt ctgacatttt
     4921 tgagaaaggc aacgggagcg gaggcgggaa cactttatct catgagcagg gaaagggata
     4981 aaatattctt catttcatca cagaacagcc gttttgactc taaaatgatc gaaaagaaag
     5041 agattccctt caacagacag agtcttgtag gatatgtgtg tcataacgga gagccattaa
     5101 acattcctga tgcttacaat attcccgagt ccgcaccgta tcagtttaac aaggaaattg
     5161 ataaaaaact caactacaaa accgtctcca taatcactgt cccgatgaaa accacaacag
     5221 gtgaaattgt cggggctgtt cagcttataa acaagatcga tgaaaggtcg aaaactgaga
     5281 tccctttcag tgattttgat gaaatgatgc tgatgagctt atccactctt gccgctgttt
     5341 cagttgagaa caataatctt cttcttgaaa ccgaaacact cctgaataac atgataatct
     5401 cttcagttaa agccatagaa cagagagatc ctgcaacaaa aggccattca ttaagagtcg
     5461 cggcatatac acttgcactt ttaaaaagag ttgcatccag accggacatt ctgaaaaacg
     5521 aaaccgttga cagatatact gttaaaactg ctgaaacggc tgcacttctt catgattttg
     5581 gaaaagtagg agtaagagaa aagatcctga tgaagcaggg gcggatatat tcagaacagg
     5641 ttgaggcggt aaaatggaga atgcggttca tcattgcttc tgtgaatgga cgggaagaag
     5701 atattgcgat cgtcagggaa atggttgaaa agatcgacgg gctgaacata ccgagacctc
     5761 ataccgatga agaaaaacag ttaattgaaa aagcaggcgg tctgacattt gaagttgacg
     5821 gaatgactgt tccggtcctt actgctgaag aacttgattt tcttaagata aagagcggaa
     5881 catttaacga ttcagaaaga gccgacatgg aaaagcatgt catctacagc catgaactcc
     5941 tcaatgcgat cgactggccg caacacctta aaaatgttcc cgaaatcgct tcatctcatc
     6001 atgaaaaact tgacggatcg ggatatcctt ttcaaaaaag tgcgaaagac ctcggaatta
     6061 ttgaaagaat acttggaata gttgatattt acgatgcgct taccgctaaa gacagaccat
     6121 acaaaaaagc aataccgcct gaaattgcaa tctcaataat agaaaaagaa gttgccgccg
     6181 gcaaactcga ccctgaaata ttcagcatcc ttgtcagaga aagagaagag atcgaacagg
     6241 aagtcagcag aaaaatcaaa gaaaataagt tgtaattatt aaagctgaat ggaggaaaaa
     6301 ttgaaaaata tcgttcttgt cattatggca gcattttcac tttttttaac aatttcctgc
     6361 gaacaggaaa aagaagagaa tatttatggt gaatatgaag acaaaaacta tgagcagccg
     6421 gatgaaaatt ttcttcccga tgaattccag aaaaaacccg atgaagatag cattgtgata
     6481 aatgacagtg agccggatga ccagagtgac tcagaatcag agtcaaccga tgaggaacag
     6541 gtcgatccgt acaaggacat ggtcctcgtt ccgcagggtt atgcatggat gggttgcaat
     6601 gaaaatgtgg aaaccgactg tcctgccgct gaactcccgt atcttgagat ctctacaaaa
     6661 gcttactaca tcgataaata tgaagtgacg gtatccgatt ttcaaaaatg cattgatgcc
     6721 aaagcatgcg gtgatgaaga gtttgcatat gatacagtta gcggcaaccc ttactgtaat
     6781 ctaggagcaa gagacacaca tccgatgaac tgtgtgagtt atttcggagc acaggcatat
     6841 tgtctctggg tcgggaaacg gcttccgagc gaaggagaat gggaaaaagc ggcaagaggc
     6901 ggctgcgaaa tacacggaga tgacaactgt gaggaaaaaa gctatatcta cacatggggc
     6961 aatgaaggtg agccaagttg cgcaaatgta agcatggctg taatggatgg acctaacatt
     7021 gattacggtt gcggatcaga ttcaaccagt gtcggaggaa gctatgaagc tgggaaaagc
     7081 ccttacggag catacgacat gctcggcaac ctttttgagt gggttgatga tgagtggagc
     7141 gctgatcaca gcacagcaaa gccagacggc tcatcaacag gatccgctga cggagaagca
     7201 gtcattaaag gcggcggatt tatgcagaca aacaaagctg aattcagaat aagttaccgt
     7261 gcaggtgcct atgttgacga gatcctcttt tccagagggt tcagatgtgt cgccgatata
     7321 gaaaattaaa caaacacttt tcctcagcca aatctttcca aaatcgtcac actttgaaca
     7381 tgtaaccaaa gacatgttca ttatttgaac acttttctgc tttttcatgt taggtttcca
     7441 aaaggtgtta tacaattgaa ctgtctgtat ttattcgata ttctttgttt gtcttccttt
     7501 ccgttatttc tggcacgcac tttgctcata tatatcggct tatgtttcgt tattcacttt
     7561 ttgaggtttg gttatgaagc tgcttacaaa gttcgttctt gcaactgttt caattttcat
     7621 aattctggct tttgccattt catatgtaag catcaagtgg ataaacagta ataccataaa
     7681 tgatgcacag caccgtgttg aactcacaat gaaatcagcc cgcgatatat tcaataagaa
     7741 aaatgacagc acgcttcagc tccttgaagt ttttgcacag aatgaggttt taagggatta
     7801 tctcaaaaac ccttcggaca aagaactttc cgaaaaagtt atggagcggt tcagaaacct
     7861 gagaatttcc tataacatgg atatccttaa cataatctca cctgacggaa cggtcatgat
     7921 gagaacaagg cctccgttca acaagggaga tgatgtttcg gacgatccca tcgttaaaaa
     7981 tgtgctgaaa aagaaaaaaa gcgtgaaggg ggtttatgtc cttacaaaaa gtcttctcat
     8041 ggatgaagga acctcgcttt accagagaac cattgacact accggtgaat cagatgcaat
     8101 gataatggga acagctattc ctgtaatgca tggagaaaaa atgatcggca tcatttcagc
     8161 cggtttcctt ctgaacggga acaatgatct cgtcgatgag atcagggatt ccatccttta
     8221 cggtgattat tataagggcc gatccgtaaa caccgttact atattcatgg gtgataaaag
     8281 gatcgcaaca aatgttgtgg gtgaaaatgc aggcagagca accgggacaa gagcttcaga
     8341 aaagatcgtg agcacagtta tgcagaacgg agacccttgg acaggaaggg cctgggtcgt
     8401 tgatgcgtgg tacctttcaa gatatgagtc gatcagaaat cctgatggcg ggatcatcgg
     8461 aatgctctat gtcggggaac ttgaagaaaa atacagagat atccgattca aggcggtgac
     8521 gagcattctt tcagttatac ttgccgcgat ggtgttcagc attctcgttt tcacattcat
     8581 tgcatggggc attcttgttc cggtgaaaaa tcttcacaat gcaactctca tgcttatgaa
     8641 cggagacaga aaggcgagga taaagggaaa ttattccgga gagataggtc accttacaaa
     8701 ctcttttaat aatatggcga atgatattga aaagcatact ctcgagatcg agaagaaaca
     8761 gaaggaactt gccgcactca acaacgagct tgaactcaca aacaggaatt atatcgatat
     8821 gctcggattt gtcagtcacg aacttaaaaa tccgctcgca agtgccgtca tgggtcttca
     8881 tactgttaaa gacggatatc ttggcgaact gaacgaaaaa cagctcaagg cacttaactc
     8941 tgttgcagaa agtctcgatt acttcaaaga tcttatcaaa aactatcttg acctttcacg
     9001 gcttgaaaaa ggggaatata tcgttcaaag acagaacatt ttccttaaaa acgccgttat
     9061 agatccggtg ctccagcagt tcactgaaga gttcaaatcc cgccacataa gcattgatct
     9121 cggggttcac gatgatacaa aagttgatgc agaccctgac ctgttaagga tcgtttatga
     9181 caaccttatc agcaatgcgg tcaaatacgg taaagaagga ggcgtgatcc ggcttcgcga
     9241 aaagatagtg gatggccggt ttgtattcag tgtgtacaac gaaggagtcg gcatacctaa
     9301 agaaaaaatg acactgctct ttaaaaaatt ctcaagaata taccgtgcag aacacgcagg
     9361 aaagagagga accgggcttg gactttattc ctgccacgag ataatcaaga aacacggtgg
     9421 aaagatatgg gctgaatcag ttcctgacaa atgggtcgag ttcagctttg aaattggcta
     9481 acggagtaaa aaatggtaaa caagatcctt attgcagatg atgatgtaca gatggttaat
     9541 gcagtgaaaa ctctacttga aaatgtggga tacgacaccg tttcaaccaa tgatcccaaa
     9601 gaagtcatcg agctggcaag aaaagaaatg cccgacctca ttatgcttga tgtcatgttc
     9661 gaggggatga ccggacccaa cggttttgaa ctttcaagaa agatcgcttc cgacaaagac
     9721 ctgaaaaaaa taccggtaat aatcttaagc ggtgtaaaaa aagtcatttc aagcgatttt
     9781 gaagtgacgc ctgacaaaga gtggcttccg gtcgccgcat ttttggacaa gccggtaaaa
     9841 cctgaccaat tgctgaagga aattaataac ttgattcaaa aaaggagttg aaaatggaat
     9901 cacaggtaaa aaaagtgctg gaaaaatatc ccggtgcagg aagagacaaa ctgataccca
     9961 ttctgcagga agttcagggt ctcgacggat ttatcagcaa aaactcggta atcgagatag
    10021 ggaagcatct gaatcttgct ccctcaaaaa ttttcggggt agccacattc tacaatcaat
    10081 tcagatttga gccgaaagga aagcatcaca tactggtctg cagaggaact gcctgccatg
    10141 tcaagggctc tgccgaaaca cttgatgcta ttgaaaggga acttggaata aaagcgggga
    10201 atacaactaa agacggactt ttcagcattg aagttgtcgc ctgtctcggt gcctgtggac
    10261 ttgcaccgct catctgcgtt gacaacgaat tttatgcaaa tgcgacaaga gaaagagtta
    10321 aagaaataat aaaaagttac cgtgcaaagg aacgggaggc gtaacatgaa aaacaatatt
    10381 gctaataact gttggagcga tgcaggaacg gcttcagatg cactgcttga aaaacttgta
    10441 aaaaaaggtt ttacgctgaa accgggaaac cctgatgaaa ttaaaaaatt aacccgtgaa
    10501 acaatcgata aaacagtgat ctatatcggt gcaggaacat gcggactcgg tgccggagcg
    10561 gctgcaacca gaaaagagat cgaaaaatat atcgcagaca aaagtatcga cgcccggata
    10621 atagaagtcg gctgcatcgg tctttgcagt gcggaacccc ttgttgacat tcagatcccg
    10681 ggcagaacaa gagtaagttt caggaacatc acatcggaaa aagttgaaaa gctttttgat
    10741 gattttttca acggtgtggt ggatgaagat aaagttttag gtcagtttca ttgccaaagc
    10801 tctctcaaat gggacaacat tcccttcctt agccagcatc ccttctttgc accacagaaa
    10861 cggtgggtgc ttatcaattg cggtatagtc gaccccttga gcatcgaaga atatgtttca
    10921 aggggcggct atgcatcttt aaatgatgct ctggctaaaa ctccgggaga tgtctgtaat
    10981 gaagttgaaa aaagcggact ccgcggaaga ggcggcggtg gctttccaac aggtaaaaaa
    11041 tggaaattcg cggctgctga aaaaagtgaa cagaaatatc tgatatgcaa tgctgacgaa
    11101 ggtgaccccg gtgctttcat ggatagggcg gttatcgaag gtgatccgtt ccggctgctt
    11161 gaaggaatga caatcgcggc ttacggaata ggtgcttcaa aaggttatat ctacatcaga
    11221 gctgaatatc ctcttgcaat agaacggctt tattttgcaa tagataaagc aaagaaatac
    11281 gggctgttag gcagcaacat cctcggaacc ggctttaact ttgatctcca gataaaaatg
    11341 ggagcaggtg cctttgtatg cggagaagag actgccctta tcaacagcat cgaaggaaag
    11401 cgcggcatgc cgagaccgag accgccatac cctgctgtca gcgggctttt cggcaaaccg
    11461 acagtcataa acaatgttga aaccctggct aat
//
LOCUS       DAOOYB010000080        11292 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_6109_length_11292_cov_12.186794, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000080 DAOOYB010000000
VERSION     DAOOYB010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11292
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6109_length_11292_cov_12.186794"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="PL056_06240"
     CDS             complement(<1..407)
                     /locus_tag="PL056_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HPM46649.1"
                     /translation="MEAKVSGRKIAFMKGNEALAEAALRAGMTGYFGYPITPQSEVVE
                     YLSREVPNHPGVCVLQAESEVAAANMLYGAGGAGGRVMTTTSSPGYSLMQEAVSYIAC
                     AEIPTVFVNVQRGGPGLGTIQPSQGDYFQAVKGG"
     gene            complement(425..649)
                     /locus_tag="PL056_06245"
     CDS             complement(425..649)
                     /locus_tag="PL056_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPM46650.1"
                     /translation="MAAKGFVEISVEGCKGCGLCIHYCPVKCLALKMDDTNSFGLHYS
                     YLAEPEKCIGCANCAMICPDGAITVYKQSK"
     gene            complement(823..1557)
                     /locus_tag="PL056_06250"
     CDS             complement(823..1557)
                     /locus_tag="PL056_06250"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004589617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HPM46651.1"
                     /translation="MKKISVFLFAAAALAIISCQMPSQKPEEKPVVKEDIQEGLNKRS
                     YALGLDISTNFKSRGVELDMRTFIAGFKSGIDGNALITEEEKMTALNELQQEMMKKMQ
                     EEMTQKSSANKEKGLKFLEENKTKEGVKVTESGLQYIVEQEGTGPNPKAEDTVEVHYT
                     GTLLDGTEFDSSVKRGQPAKFPLNRVIKGWTEGLQLMKKGAKFKFFIPSDLAYGDYGS
                     QNIGPGETLVFEVELLSIEKAAPAKK"
     gene            1733..3160
                     /locus_tag="PL056_06255"
     CDS             1733..3160
                     /locus_tag="PL056_06255"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPM46652.1"
                     /translation="MFAMLKNYFSEFKILKTVSKEFWLVNAIQFFDGLSYFSVIIVIS
                     MYLTENVGFSDYDAGVWQGIFFLFITAFMFTVGSICDAIGVKKSFLFAAFLLMVSRLG
                     MGVGPEMFAGDALKYVIIAMLLIMALGTAVLVTNTNTALRHFTSKENRATGFNVYYLI
                     MNIGAAIAGFGVTDGFRNWLGTVKGNLAIFTFGFVMSAVCFVAAYMINESNREEKEEP
                     KEPGKSKTPIQIFAEVWKEKPFQKLVFFLVLTLGVRLVFTHQTMVMPKYYLRTLYADF
                     QLGAVNSLNPIIIVVGLILVIPILNKFNIVKLLVVGMGISAVSLLFLAVPGEWITAVP
                     GIRNLDQAYYWVIVLQILVFAVGELIFSPRFVEYVASVAPKDKVASYMGLSALPMFIA
                     RPLNGFVSGLLIAGLSYDGIRAKIDTGNIDYLNSPEFMWMIYFALAIMSPVAVIALKK
                     SLSQENIDEAPSSEPQTASQTEEAN"
     gene            3171..3956
                     /locus_tag="PL056_06260"
     CDS             3171..3956
                     /locus_tag="PL056_06260"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HPM46653.1"
                     /translation="MTNYKAIIRDGLILMVISTVFALVFKVGFDQLRGQVQDDPLITA
                     SLQDNIDMMITTLSEESFKKDPQGKQTYEEFTKLMTNKQDEQKRSPIMWVAYVNFYDR
                     QIIKSKEEKRLVAAKLLLEKGADIGLKDEHGWTALSWASWTGFPTLVSLFIEKGADLN
                     ITDLKGQTPLMIASIRSNDEVVRILLEKGADREIKNSEGKNALDFAQEYLNRYPERKS
                     NYENIISSLQTVVKVEEPKPAEEPQAAEEEKPATEVPVEQKAE"
     gene            complement(3960..4910)
                     /locus_tag="PL056_06265"
     CDS             complement(3960..4910)
                     /locus_tag="PL056_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018125661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HPM46654.1"
                     /translation="MKSPGPNSLKEAAVLAAKGLCMGGADIIPGVSGGTIALIAGIYQ
                     KLLAAIKSVDIVFFKKLLKFDIKGAIAEVHIRFLVVLFSGIAVAIISLSRLMNFLLKN
                     HQPPTFSLFYGLIAASIYVVGKQVKWKTAEYISIVAGIVMAYLIVGLIPVSTPTGLWF
                     IFLSGVIGICAMILPGISGAFILLILGKYEFITGTLKSPFSVNEITGVNNLIIIIIFC
                     LGCAAGLAGFSRFLNWLLSKWHSITLAFLTGLMAGSMRRIWPWKGEAVIEIIRGKEHI
                     VSQPNVFPESFGLSFVFCLFLMVLGIAVIIILDRFSKRFS"
     gene            complement(4958..5719)
                     /gene="surE"
                     /locus_tag="PL056_06270"
     CDS             complement(4958..5719)
                     /gene="surE"
                     /locus_tag="PL056_06270"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF001490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HPM46655.1"
                     /translation="MTNDDGIHAAGLNAVEEDLESRGIPFFTAAPLYENSGAGHSITL
                     DKPLKIKKFSNNRFGVTGTPTDAVFLGLHELLKEKPVFAVSGINKGGNLGNDITYSGT
                     VAAAIETFYNGITSFAVSLYISDKESFCDDLFSIAAKVLFDIIIPDIEVKIGKNELYS
                     IPHLFNINIPDTALKKKDYKIEWTSLGKRLYGGEVVKRTDPRGGEYYWIGGNQHLFEN
                     IEGSDCNAVSEGFISISPLKLSFSNDSLLEKIKNN"
     gene            complement(5751..5828)
                     /locus_tag="PL056_06275"
     tRNA            complement(5751..5828)
                     /locus_tag="PL056_06275"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5791..5793),aa:Pro,seq:ggg)
     gene            complement(5878..6165)
                     /locus_tag="PL056_06280"
     CDS             complement(5878..6165)
                     /locus_tag="PL056_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HPM46656.1"
                     /translation="MSVTKVELAELVYEQMKVDKRHAAELVDMFFEELKTGLVNDGEI
                     QISGFGKFVVKDKKERKGRNPQTNKPLTISKRKVATFHHSQVLKDRLNSKK"
     gene            complement(6182..6532)
                     /gene="rplT"
                     /locus_tag="PL056_06285"
     CDS             complement(6182..6532)
                     /gene="rplT"
                     /locus_tag="PL056_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015902141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HPM46657.1"
                     /translation="MRVKRGFKAKRRRATIMKAAKGNYQSRSKTYRVAKETVERGWAY
                     AFAHRKLKKRDFRKLWITRINAAVRERGLTYSTFIDMLNKANIDIDRKVLAFLAVEDP
                     GAFDKVIEEARAVN"
     gene            complement(6550..6747)
                     /gene="rpmI"
                     /locus_tag="PL056_06290"
     CDS             complement(6550..6747)
                     /gene="rpmI"
                     /locus_tag="PL056_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004223312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HPM46658.1"
                     /translation="MPKMKTNRAAAKRVKSNGSGKLKRWHAFKSHILTKKSRKRKRDL
                     RKGVMIENCDVPAFKKLLPYG"
     gene            complement(6761..7315)
                     /gene="infC"
                     /locus_tag="PL056_06295"
     CDS             complement(6761..7315)
                     /gene="infC"
                     /locus_tag="PL056_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013933182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HPM46659.1"
                     /translation="MKNSKAESNGAAASQGVGLVNETIKSGTMRVIAPTGEVLGVLAK
                     RDALEKAREFGLDLVCVSPNTDPVVCKIMDYGKHLYKMKKKAKKAKANQVVVEIKELK
                     IRPNTDTHDLMTKVRQAKKFLEEGNKVKFTVFFRGREIMFAEKALENLNLIVKELGGE
                     DTLQIELDSIVKNKKMTMMVSQKN"
     gene            complement(7315..9228)
                     /gene="thrS"
                     /locus_tag="PL056_06300"
     CDS             complement(7315..9228)
                     /gene="thrS"
                     /locus_tag="PL056_06300"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HPM46660.1"
                     /translation="MFEVTLPDGRIVSSEKAENWLEFIKREIGAGLAKAAVAVSVNGK
                     TVDVTSNVETGGMTVITTKSPEGLEIIRHSTSHVMADAVQKLFPGTKVTIGPAIDNGF
                     YYDFDSPHRFSQEDFEAIEMKMADIIKAKHPFIRKEVSREEAIELFTNMGENYKVEII
                     QDLNEPVVSLYFSGDFVDLCRGPHVPDTGFIKAYKLMSVAGAYWRGDEKNAMLQRLYA
                     TGFATKEALEAHIKMLEEAKERDHRKLGADLDLFDFSENVGAGLVLWTPNGGVVRTVV
                     ENFWRKQHVRNGYELVFTPHIGRSHLWETSGHLGFYKESMYSPMDIDGEDYYVKPMNC
                     PFHVMLYKRKKWSYREFPFRWAELGTVYRYEKSGTLNGLKRVRGFTQDDAHLFCRLDQ
                     LEDEIKRVLDFSLFMLRSFEFNEFKLFLSTRPQKYVGQIEVWDKAEEALRNTLEKSGL
                     SWETNEGDGAFYGPKIDINVTDSIGRLWQLTTIQVDFNLPERFDISYTGDDGAEHRPI
                     MVHRALLGSIERFFGVLIEHFKGAFPVWLSPVQVAVLPVSDRHNATASKFRDMLVENG
                     IRVKFDERNEKIGYKIREWVMEKTPYIVVIGDNEADLKIISVRNRSGEQEAVTVEDFI
                     NMLNKENFNGVYY"
     gene            complement(9263..9337)
                     /locus_tag="PL056_06305"
     tRNA            complement(9263..9337)
                     /locus_tag="PL056_06305"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9303..9305),aa:Val,seq:gac)
     gene            9546..10799
                     /locus_tag="PL056_06310"
     CDS             9546..10799
                     /locus_tag="PL056_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015283416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HPM46661.1"
                     /translation="MGQTFAEKIFSKMAGRPVVPGTILTIRPDHVLTHDNSSAIFSTF
                     KKMGGKKVNDPDQIIIILDHIVPASDEKAATNHQTVRKFVKDQGVRNFYDGGRGICHQ
                     VVIEEGFALPGKFIVGSDSHTTTYGVMGAFSTGIGRSESAGIWATGEIWLKVPESMKI
                     VMNGKPKPFVSSKDVLLHIAGLINVDGGLYKSVEFSGDYIDSIELADRMIFTNMSVEI
                     GAKNGYIAPDKKVFDYLEKRGVTKDKYDVIYPDADAEYAEVLEIDVSAIEPVVAAPHS
                     VDIIRKVSEVKDLKVDQVLIGTCTNGRIEDFRIAAKILKGKKIARDTRLLILPASSEI
                     YLAAMEEGLFKVFIEAGGIILNPGCGPCLGAHEGIMAPGEVTVSTANRNFKGRMGCNQ
                     SDIYLASPAVAAATAIKGFLTVPEN"
     gene            10815..>11292
                     /locus_tag="PL056_06315"
     CDS             10815..>11292
                     /locus_tag="PL056_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase"
                     /protein_id="HPM46662.1"
                     /translation="MAIIKGKVFVYGHDVNTDVIFPGKYTYTVTDPKEIASHSLEDED
                     PAFIKEVQPGDIIIAGKNFGCGSSREQAAFCFKYLGMGAIIAESYSRIYFRNCINAGL
                     PALEIKNLLGKVKRGDTVEINTETGTVTLPDGSKVTFPPLPPEVDEIMQCGGLDEYI"
BASE COUNT         3223 a         2485 c         2211 g         3373 t
ORIGIN      
        1 ccgcctttaa ctgcctgaaa ataatcgccc tgactcggct gaattgttcc aagacctgga
       61 ccgccgcgct gaacattgac aaaaacagtg ggaatttcag cgcatgctat atatgaaacc
      121 gcttcctgca tcaacgaata gccgggagat gatgttgttg tcataactct tccgcctgct
      181 ccgcctgctc cgtaaagcat attagctgca gcaacctcac tttctgcctg aagaacacaa
      241 actcccggat ggttgggaac ttcacgggaa agatattcaa caacttctga ctggggagtt
      301 atcggatatc cgaaataacc ggtcatgccg gctcttaaag cggcctccgc cagagcctcg
      361 tttcctttca taaaagcaat cttccttcca gacacttttg cctccatctt attgaaatta
      421 ttaactactt tgactgtttg taaactgtta tcgccccatc agggcagatc atggcacagt
      481 ttgcacaacc gatacatttt tccggttcgg caagatatga ataatgcaga ccgaaagagt
      541 tggtgtcatc cattttaaga gcaagacatt ttaccggaca ataatgaatg caaagaccgc
      601 agcctttaca accttcaact gaaatttcca caaatccttt tgctgccatt tcttcctcca
      661 agaattgaaa ttgaaattaa caaacgcact ggtgaatgta tcacctaaag atataaaaat
      721 caagtgggaa aacgcaaaaa atagagcatt attaactttt gttgctaatg agattaagcg
      781 tgttatttca gagtgttata aaaatttgct caaattgggc aattattttt ttgcaggagc
      841 cgccttttca attgaaagaa gttccacttc aaaaacgagt gtttcgcccg gtcctatatt
      901 ctgactgccg tagtcaccgt atgcgaggtc tgaaggaatg aaaaatttga attttgcccc
      961 tttcttcata agctgcagac cttccgtcca gcccttgata actctgttga gggggaactt
     1021 agccggctgg cctcttttta cagaactgtc aaattcggtc ccgtcaagaa gagttcccgt
     1081 ataatgaact tcaactgtat cctcggcttt ggggttcgga cctgttcctt cctgttcaac
     1141 tatgtactgg agccccgatt cagtgacctt cacgccttct tttgtcttgt tttcttcgag
     1201 aaacttcagc cctttttctt tgtttgctga tgatttctgc gtcatctcct cctgcatttt
     1261 cttcatcatt tcctgctgaa gttcattcag agctgtcatt ttctcttctt cagtgataag
     1321 agcattgccg tcaataccgg acttaaaacc ggcaatgaaa gtcctcatat caagttcaac
     1381 accccttgat ttaaaatttg ttgaaatatc aagaccgagt gcataacttc ttttgttaag
     1441 cccttcctga atgtcctctt tgacgaccgg tttttcttcg ggcttctgag aaggcatctg
     1501 gcacgaaatg atcgcaagag ctgccgctgc aaataaaaaa acggaaattt ttttcatcat
     1561 atctctcctt gagattaaag ttaacaaaaa gacccgaaac attaccagaa aaaacatttt
     1621 tagcaaaatt atttagtgct atccgtaaaa ttcacgcagc cattaagttt tttttgactt
     1681 acgatgactt ttctaagaaa cttacccgac tttttttttg gaggagcgat caatgtttgc
     1741 aatgttgaaa aactattttt cagaattcaa gatccttaaa acagtaagta aggaattctg
     1801 gcttgtcaat gctattcagt tctttgacgg tctatcttat ttttcagtta ttattgtcat
     1861 ttccatgtat ctgactgaaa atgtaggatt cagcgattac gatgccggtg tatggcaggg
     1921 gattttcttt ctatttatta ccgcgttcat gtttacagtc ggttcgatct gcgatgcaat
     1981 aggtgtgaaa aaatcattcc tttttgccgc atttcttttg atggtaagcc gtcttggaat
     2041 gggtgtagga ccggaaatgt ttgcaggaga tgcactgaag tatgtcataa tcgcaatgct
     2101 tctgatcatg gctcttggaa cagcagtgct tgtcacgaac accaatactg cacttcgtca
     2161 ctttacttca aaagagaaca gagcgaccgg gtttaatgtc tattatctga taatgaacat
     2221 cggtgccgcc attgcagggt tcggtgttac cgacggattc aggaactggc tcggcacagt
     2281 taaaggcaat ctcgctattt ttaccttcgg ttttgtaatg agcgcagtct gttttgttgc
     2341 cgcatatatg ataaatgaaa gcaaccgtga agaaaaagaa gaaccgaaag aacccggaaa
     2401 atcaaaaact ccgatacaga tatttgcaga agtatggaag gaaaagccat tccagaaact
     2461 tgttttcttc cttgtgctta ctctcggagt ccggcttgtt ttcactcacc agacaatggt
     2521 tatgcccaaa tattatctga gaactcttta tgctgatttt cagcttggtg cagttaactc
     2581 attaaacccg ataattatag ttgtgggact tatccttgta attccgatac ttaataaatt
     2641 caatattgtt aaacttcttg ttgtcggcat ggggatttca gccgtttccc ttcttttcct
     2701 tgcagttcca ggagaatgga taaccgcagt tccaggcatc agaaatctcg atcaggcata
     2761 ttactgggtt atcgtacttc agatccttgt ttttgcagtt ggagaactta ttttctctcc
     2821 aagatttgtt gagtatgtcg catccgttgc tcctaaagac aaagtcgcgt cctacatggg
     2881 gctgtctgcg ttgccgatgt tcattgccag accattgaac ggttttgtaa gcgggcttct
     2941 tatcgcgggt ctttcgtatg acggaataag agcaaagatc gacacaggga atattgatta
     3001 tctcaacagt cctgagttca tgtggatgat atactttgct cttgccataa tgagccccgt
     3061 tgctgtaatt gcacttaaaa aatctctttc tcaggaaaac attgatgaag ctccttcttc
     3121 agagccgcaa acggcatctc aaactgaaga agcgaattaa ggagggtaaa atgacaaatt
     3181 ataaagcaat aataagagat ggccttattc tcatggtcat cagcacagtg ttcgccctcg
     3241 tattcaaagt cggatttgat cagttaagag gccaggttca ggatgatccc cttataactg
     3301 catcgcttca ggacaacatt gatatgatga taaccacttt aagtgaagaa agctttaaga
     3361 aagatccgca gggcaaacag acatatgaag agttcacaaa actcatgacc aacaaacagg
     3421 atgaacagaa aagatctcct ataatgtggg ttgcatatgt aaatttttat gatcgccaaa
     3481 taataaaaag caaggaagaa aaacggcttg ttgctgcaaa actgctgctt gaaaaaggtg
     3541 cggatatcgg cttgaaagac gagcatggct ggaccgcttt gtcatgggcg tcatggacag
     3601 gttttccaac actggtttca cttttcatcg aaaaaggggc tgatctgaat atcactgatc
     3661 ttaagggtca aacgccgctg atgatagctt ctataagatc taatgatgag gttgtcagaa
     3721 tacttcttga aaagggtgct gacagagaga tcaaaaacag tgaaggtaaa aacgctcttg
     3781 attttgcaca ggaatattta aacaggtatc ctgaaagaaa atcaaactac gaaaatataa
     3841 tttcgtccct tcagacagtt gtaaaagttg aagaaccgaa acctgctgaa gaaccgcaag
     3901 ccgctgaaga ggaaaaacct gcaactgaag ttcctgtaga gcaaaaagcc gaataatttt
     3961 taagaaaatc tctttgaaaa acggtcaaga ataattatca cagcaattcc aagaaccata
     4021 agaaacagac agaacacaaa agagagtccg aaactttcag gaaaaacatt cggttgagaa
     4081 acaatatgtt cctttccacg aatgatctca ataactgctt cgcctttcca cggccagatc
     4141 cttctcattg agccggccat gagtccggtt agaaaagcaa gtgtgatgct gtgccattta
     4201 gataaaagcc agtttaaaaa tcttgaaaaa ccggcaagcc ccgctgcaca gcccagacag
     4261 aagatgataa tgataatgag attgttcact cccgttattt cgtttacaga aaaaggtgac
     4321 ttcagggttc cggtaataaa ttcatatttt ccaagtatca gcagaatgaa cgcaccgctt
     4381 attcccggaa ggatcatagc gcagatcccg ataactccgc ttaagaagat gaaccacagt
     4441 ccggtgggag ttgaaacagg aatgaggccg acaatgagat acgccattac aattccggca
     4501 actattgaaa tgtactcagc tgttttccat tttacctgtt ttcctaccac atatattgat
     4561 gcggcaataa gtccgtagaa caaactgaat gtcggtggct gatgattctt taaaagaaag
     4621 ttcatcaacc ttgaaagaga tatgatcgca actgcaatac ctgaaaaaag aacaacaaga
     4681 aaccttatgt gaacttcagc tattgcccct tttatatcaa atttcagaag ttttttaaaa
     4741 aaaacaatgt caaccgattt aattgccgca agaagcttct gataaattcc tgctataagg
     4801 gcaatagttc ctcccgaaac accgggaatt atgtcagctc cgcccataca gagacctttt
     4861 gcagcaagaa ctgccgcctc tttaagggaa tttggaccgg gagatttcat aactgcctgc
     4921 ctgaaagtca ttttttccat ttttcacctt attttaatca attgtttttt attttttcaa
     4981 gaagagaatc gttggaaaaa gaaagtttca acggagaaat gcttatgaat ccttcgctga
     5041 cagcattaca atcgctccct tcaatatttt caaaaagatg ctgattgcct ccgatccagt
     5101 agtattctcc acctcgggga tctgttcttt taacaacttc tccaccgtaa agtctcttac
     5161 ccagagaagt ccactcgatt ttataatcct tttttttcaa agctgtatca ggaatgttga
     5221 tgttaaaaag atgcggtatt gaataaagtt catttttccc tatctttact tcaatatcag
     5281 gaattataat gtcaaaaaga acttttgccg caatactgaa aagatcatca caaaagctct
     5341 ctttatctga aatgtaaagt gaaaccgcaa atgaagttat gccgttataa aaagtctcaa
     5401 tagctgcggc gacagtaccc gaataagtta tgtcatttcc gagattgccc cccttgttta
     5461 ttccggaaac cgcaaatacc ggtttttctt ttaaaagctc gtgaagacct aaaaagactg
     5521 cgtctgtcgg agtccctgtt acaccgaaac gattgttgga aaatttcttt attttcagag
     5581 gtttgtcaag agttatcgag tgacccgcac cgctattttc ataaagcggg gcagcggtaa
     5641 aaaaaggaat gcccctgctt tcaaggtctt cttcaactgc attaagtccg gcggcatgaa
     5701 taccgtcgtc atttgtcaat aatatttttt cgttcacaat attttaaaag tggtcgggat
     5761 gactggattt gaaccagcga ccacttgaac cccattcaag tacgctaccc aggctgcgct
     5821 acatcccgac ctttaaaaac aaaatcctga aaaaaatcag gacttgtttt ataaacttta
     5881 tttcttgctg ttaagccggt ctttaagcac ctggctgtga tggaatgtcg caaccttacg
     5941 cttgctgata gtcaacggct tgtttgtctg gggattacga ccctttcttt ctttcttgtc
     6001 ttttacgaca aattttccga atccgctgat ctggatctca ccatcattta ccaatcctgt
     6061 tttaagctct tcaaagaaca tgtcaacaag ttctgctgca tgccttttgt caaccttcat
     6121 ctgctcgtaa acaagttccg caagttctac tttagttacg ctcattttgt cccccaattt
     6181 atcagttaac ggctctggct tcttcgatca ccttatcgaa agccccggga tcttcaactg
     6241 caagaaatgc aagtactttt ctatcaatat ctatatttgc tttattaagc atgtcaataa
     6301 atgttgaata ggtgagacct ctttctctga cagctgcatt gattcttgta atccaaagct
     6361 ttctgaaatc tctcttttta agttttctat gcgcaaaagc ataggcccag cccctttcga
     6421 ctgtttcctt ggcaactctg taggtctttg accttgactg atagttgcct tttgcggcct
     6481 tcattatcgt ggctcttctt ctttttgctt taaatcctct tttaactctc attttggact
     6541 cctttaattt taaccgtacg gcagaagttt cttgaatgcg ggaacatcac agttctcgat
     6601 cattacaccc tttctcaaat ctctttttct cttacggctt ttctttgtaa gaatgtgact
     6661 tttaaaagca tgccatcttt taagttttcc gctaccgttg gatttaactc tttttgcggc
     6721 tgctctattg gttttcattt tgggcatagc actctctcca tcagtttttt tgcgatacca
     6781 tcatcgtcat ttttttattt ttcacgatac tgtcaagttc gatctgaaga gtgtcctctc
     6841 cacctagttc cttaacgata agattgagat tctccaatgc tttttcagca aacatgatct
     6901 ctcttcctct aaaaaaaaca gtgaacttga ccttgttccc ttcttcaaga aattttttgg
     6961 cctgacgaac tttcgtcata aggtcgtgcg tgtcggtatt cggtctgatc ttcagctcct
     7021 tgatctcgac aacgacctga ttggccttcg ccttcttggc tttctttttc atcttgtaca
     7081 gatgtttacc ataatccatg atcttgcaca caacggggtc tgtgttcggg gaaacacaaa
     7141 cgagatccag cccgaattct cttgcttttt ccaaagcatc cctttttgca aggacaccca
     7201 gtacctcacc ggtcggagct attactctca tggtgccgga tttaatcgtc tcattgacaa
     7261 gtccaacacc ctgggaagct gccgctccat tggattcggc tttactgttt ttcatcaata
     7321 gtaaactccg ttaaaatttt ccttgtttaa catatttata aagtcttcga ctgtaacggc
     7381 ttcctgttca ccgcttctat tccgtacact tattatttta agatcggctt cattatcccc
     7441 gataacaaca atgtacggtg ttttttccat aacccattct ctaatcttat aaccgatctt
     7501 ttcatttctt tcgtcaaatt tcactcttat cccgttctca acaagcatat ccctgaattt
     7561 ggaagctgtt gcgttgtgtc tgtcagaaac aggaagaacc gccacctgta ccggagaaag
     7621 ccacacaggg aaagctcctt tgaaatgttc aataagaact ccgaaaaatc tttcgataga
     7681 accgagaagc gctctgtgaa ccattatagg tctgtgttct gctccgtcat caccggtgta
     7741 tgaaatatca aatctttccg gaaggttgaa gtcaacctga attgtggtaa gctgccagag
     7801 ccgaccgatc gaatcggtga catttatatc tattttaggt ccgtagaaag ctccgtcacc
     7861 ctcatttgtc tcccatgaaa gtccgctttt ttcaagcgta tttctaagtg cctcttctgc
     7921 tttatcccag acctctatct gacctacata tttctgtgga cgggtgctca aaaacagttt
     7981 aaattcattg aattcaaagc ttcttaacat gaaaagggag aaatcaagaa ccctttttat
     8041 ctcatcttca agctgatcaa gccggcagaa aagatgtgca tcatcctgtg tgaatccgcg
     8101 aactcttttc agtccgttaa gagtgcccga tttttcatat ctgtaaaccg ttccaagttc
     8161 agcccatctg aaaggaaatt ctctatatga ccattttttc cttttataaa gcattacatg
     8221 gaatggacag ttcatcggtt ttacataata atcctctccg tcgatatcca tcgggctgta
     8281 catactttct ttataaaaac cgagatgtcc gcttgtctcc cagagatgac tgcgacctat
     8341 atgcggagtg aaaacaagtt catatccgtt cctgacatgc tgttttctcc agaaattctc
     8401 aacaacagtt cttactactc cgccattggg tgtccataac acaagtccgg caccgacatt
     8461 ttcagaaaag tcgaaaagat caaggtcggc tccaagtttt ctatgatctc tttcttttgc
     8521 ctcttcaagc atttttatat gtgcctcaag ggcttctttt gttgcaaagc cggtcgcgta
     8581 aagccgctga agcattgcgt ttttctcatc acctctccaa tacgcaccgg caaccgacat
     8641 gagtttatac gcctttataa aaccagtgtc aggaacatga ggaccgcggc agagatcaac
     8701 aaaatcaccc gaaaaataaa gagataccac aggttcgtta agatcctgaa ttatttcaac
     8761 tttataattt tcacccatat tggtgaaaag ttcgatagct tcttctctgc tgacctcttt
     8821 cctgatgaaa ggatgttttg ctttgattat atcggccatc ttcatttcga tcgcttcaaa
     8881 atcttcctgt gagaaacggt ggggagaatc gaaatcgtaa taaaatccgt tatctattgc
     8941 cggacctatt gtgaccttgg ttccagggaa aagtttctga accgcatcgg ccatgacatg
     9001 cgaagttgag tgcctgatta tctcaagtcc ttcagggctt tttgtcgtga tgactgtcat
     9061 gccgcctgtt tctacatttg aagttacatc aacggttttt ccgttcacag aaacagctac
     9121 agccgctttt gccagacctg caccgatctc tcttttaatg aattcaagcc agttttctgc
     9181 tttttccgaa cttacaatcc tgccatccgg aagagtcact tcaaacattc aaccctctta
     9241 atgttgtctt cccccctgat aatggtaggc ccgagtggga tcgaaccact gacccctgct
     9301 acgtcaaagc agtgctctcc cgctgagcta cgagccccca gaacaaggaa aaagtgctga
     9361 tttttatagc agttagccct attattgtca atcttttttc aagaaacagt gctaagttgc
     9421 tgaatctaaa ggaaaaataa tgtctgcaac atatatttga aagataaaat ttgtgttatt
     9481 tattaaaaat gtaaaaatgg tccggacggg ttctttaaac ttctaatttt gcgaggtaac
     9541 gggaaatggg acagactttt gctgaaaaga tcttttcaaa aatggcaggg agacctgttg
     9601 ttccgggtac gatccttacg atcagacctg atcatgtgct gactcacgac aactcaagtg
     9661 ctattttttc gactttcaaa aaaatgggcg gaaaaaaagt aaatgatcct gatcagatca
     9721 ttatcatcct tgaccacatt gttccggcaa gtgacgaaaa ggcggcgaca aatcaccaga
     9781 cagtaagaaa atttgtcaaa gatcaagggg ttagaaattt ctatgacggc ggaagaggga
     9841 tctgtcatca ggttgtaatt gaagaaggtt tcgcacttcc gggcaaattt atcgtcggaa
     9901 gcgactccca caccacaact tacggtgtca tgggtgcttt ctcaaccggt atcggaagaa
     9961 gtgaatcggc aggtatctgg gctacaggcg agatctggct taaggttcct gaatcaatga
    10021 agatagttat gaatggaaag ccgaagccgt tcgtttcatc aaaggatgtt ctcctgcata
    10081 ttgcagggct tatcaatgtt gacggtggct tatacaaaag tgtagagttt tcaggtgatt
    10141 acatcgactc catcgaactt gcggacagga tgatattcac aaatatgagc gtagagatcg
    10201 gtgcaaagaa cggttatatc gcccccgata aaaaagtttt tgactatctt gaaaaaagag
    10261 gagtaacaaa agataaatat gatgtcatct atcccgatgc agatgctgaa tatgctgaag
    10321 ttcttgaaat agatgtatcc gcaattgaac cagttgttgc cgcaccgcac tctgttgaca
    10381 taatccggaa ggtttctgaa gtaaaagatc ttaaagtaga tcaggtactt ataggaacat
    10441 gtaccaacgg ccgtattgaa gatttcagga tcgccgcaaa aatactgaaa ggaaaaaaga
    10501 tagctcgcga tacacggctt ctgatacttc cggcaagttc tgaaatatat cttgccgcaa
    10561 tggaggaagg tcttttcaaa gtattcattg aagccggcgg aataattctc aatcccggtt
    10621 gcggaccgtg tcttggtgct cacgaaggaa taatggctcc cggcgaagtg acagtttcaa
    10681 ccgcgaacag aaacttcaag ggaaggatgg gctgcaatca gtctgacatc tatcttgcaa
    10741 gtccggcagt tgccgcggct acggcgatca aaggattttt aactgttcct gaaaattaac
    10801 tgtaggagga gaatatggca ataataaaag gaaaagtttt tgtttacgga catgatgtca
    10861 atacagatgt cattttcccc ggcaaatata catacacagt gacagatccc aaagaaatcg
    10921 caagtcactc tcttgaagac gaagatcctg ctttcattaa agaagttcaa cccggtgaca
    10981 tcataatagc cggaaaaaat ttcggttgcg gatcatcgcg tgaacaggcg gcattctgct
    11041 tcaaatatct cggaatggga gcaataatcg ctgaatcgta cagcagaatt tatttcagaa
    11101 actgcatcaa cgcaggactc cccgctctcg aaataaaaaa tcttctcgga aaagtaaaaa
    11161 gaggcgacac cgttgagatc aacaccgaaa caggcacagt tacactccct gacggctcga
    11221 aagtgacatt ccccccgctt ccgccagaag ttgacgagat catgcagtgc ggcggactcg
    11281 acgaatacat ca
//
LOCUS       DAOOYB010000081        11282 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_6125_length_11282_cov_10.664648, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000081 DAOOYB010000000
VERSION     DAOOYB010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11282
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6125_length_11282_cov_10.664648"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(320..628)
                     /locus_tag="PL056_06320"
     CDS             complement(320..628)
                     /locus_tag="PL056_06320"
                     /inference="COORDINATES: protein motif:HMM:NF013555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HPM46663.1"
                     /translation="MNTGWVICKGCGAEYFIEHKKYPVKDEGDSLSCEKCGELLHKWG
                     KGTDDYILFTKEQMQKIEKEKALIPRCDCGAKMLKRKVKWGYFWGCSKFPNCQKTKKI
                     "
     gene            complement(686..2128)
                     /locus_tag="PL056_06325"
     CDS             complement(686..2128)
                     /locus_tag="PL056_06325"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HPM46664.1"
                     /translation="MAIINCPECGKKISEYAERCPSCGLPQDKFIKDKQIGVQEKEET
                     TKVTKTRKFIINKVVFLMAFLLFTIIVATILLISDDDEIVMSFKVNNKYGLIDLNGNI
                     LIEPKMEYLGTFFDGLAPASNEDGKVGFINKRGDFIIKPIFDFASSFYSSANAAVVMI
                     NGLWGVIDRKGDFIVQPLHELAETIPKSNEYFMFMKNGKYALFRKNVRITDYVFDEGH
                     GNFDESRYIVARKEGKLGVIDLEGNTIIDFKYDSLTIPSKSESNFVVFKKDFKTGVLN
                     KKGEEVLPAGLYDFISSFSNGRAFARKGEDIYLLDENHKIIKTLKHGLEFYYWGNKNV
                     VVYKDDKFGLIDYNGEIIFEPKYEEIEPIENGKYLVKVNGSYGVFNEKEEYLIPPIYS
                     KLYGTIDFSDMIYFLGTALKKHGKDLYGMIDDKVLKNLEKAGQNLTIECFEEFLKLLS
                     ALSANDLNKENEERILNHSKRFVEECTSLY"
     gene            complement(2152..2445)
                     /locus_tag="PL056_06330"
     CDS             complement(2152..2445)
                     /locus_tag="PL056_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46665.1"
                     /translation="MGHNDHEDRGFKDFLQQIVEGGFIEQPVLGITKQVISKGEESLS
                     EKQKAVFRRKVVDFYKGIKCKRCGDDISWSEKYDAISDHGMCNYCSHKTEKGE"
     gene            complement(2632..2991)
                     /locus_tag="PL056_06335"
     CDS             complement(2632..2991)
                     /locus_tag="PL056_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HPM46666.1"
                     /translation="MIRDTLLYLDDISDAIKKIESFISEITEEQFFKDDKTIFAVTRA
                     LEIIGEAVNKIPEELKEKYPEIPWKDIYGMRNVLIHAYFGVDIHVLWKTLHTDIPQLQ
                     ETVNKIRITEFPDEIEG"
     gene            complement(2988..3290)
                     /locus_tag="PL056_06340"
     CDS             complement(2988..3290)
                     /locus_tag="PL056_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010869621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HPM46667.1"
                     /translation="MVAKKINTSEVIALLRVNKDFLRSLKIKSIYLFGSYARNEQTDK
                     SDVDLIVEFDVVPSLFEFIRVQRELSNLLGIKVDLVIKDSLKPRVAKTALKEALAI"
     gene            complement(3394..4608)
                     /locus_tag="PL056_06345"
     CDS             complement(3394..4608)
                     /locus_tag="PL056_06345"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPM46668.1"
                     /translation="MKKLILVQLFVFIVLTGCDNNFRKIVVPEAEVKNDSDNSDIMIP
                     DNDESDDDLVDQDDLSDDLPDDLSDDLSDEYLAESEVLTESEEEPDEDDYDGFYDVPS
                     DPSPIDLNGVEARFHADIAYGDKQLNTFDIFIARTKEPAPLVIYIHGGGFTMEDKSDA
                     YEAGGPFVIRSLLENGISFASVNYQLLEQNDKDGVIKPLNDCKRALQFIRSESEVFNI
                     DKEKIVSYGFSAGAGASLWIGFNDDMADPESEDPVLRESTRLKGVAALETQATYDIVR
                     WETEIFKPYDITLKSFVNLGFGPTLYSFYGVSSLDEIYTPAITEYRKKVDMLDLMSAD
                     DPEIFVNNAYDDAGKPTNLYALYHHPYHASALMEEAEKKGLQGIFYIPAMNIIDESNE
                     ELIQFFLRKLKY"
     gene            4755..5396
                     /locus_tag="PL056_06350"
     CDS             4755..5396
                     /locus_tag="PL056_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46669.1"
                     /translation="MNKALLIVILLFCLFNISASEQSGKDGWTLKKKEDRYQIYQKKT
                     PGSDFKDAKTIALITGSIDESFKVVTDPANFTALFNPHVERSAVLKKKEKCNEIYILI
                     NPPLFSRRETTVEVCYSDLSETSFKLNWKNSDTNNSAKSEYVRMNTFSGTCSFSRTEA
                     NDELKVECITSLDLGGSIPIFTINDINSRAVVQFIWKIYHEILNQRKNKGGQK"
     gene            5393..6238
                     /locus_tag="PL056_06355"
     CDS             5393..6238
                     /locus_tag="PL056_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46670.1"
                     /translation="MKKLIVSASIFLFAGFFLFAADDPAQKEEEKKDEKSSSGWFGEP
                     EPAMLELGLGFFYHTYHFETHDDDGRDETQINYRKDLGYEKAYLHFDADFAFRFKKFK
                     LSAGYTMLNAVSKKAAGRELTFYDAIFNMGEDMKSTFNVHLIKLDFTWYILDLDVGKN
                     LNFKLGPSIRIDMFVTDIRLESTEDKSKKDKFISPVVPFPSLGISFETTIFKYSGIFF
                     DINGMYAGKYLGFMDLKSGIRVYPWHWTGIEFAYRHLLAKALWQGDGVETEFKGFNMS
                     FILRF"
     gene            6247..>6476
                     /locus_tag="PL056_06360"
     CDS             6247..>6476
                     /locus_tag="PL056_06360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009725094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPM46671.1"
                     /translation="MSCPLISAIIPVFNRPELVKRAINSILSQTYKNFEIIVVDDGST
                     DETPVVLKGYDGRINAIRTENKGVSAARNTAV"
     assembly_gap    6477..6576
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6730..7584
                     /gene="speB"
                     /locus_tag="PL056_06365"
     CDS             6730..7584
                     /gene="speB"
                     /locus_tag="PL056_06365"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HPM46672.1"
                     /translation="MFQIPRFISSRENPEEASIIVAGIPYDCTSSFRSASRFGPRELR
                     NYAFEAIEEFSFYQGKTLDDVPFCDVGDLEVMVGNPANMVKTVMEEVKPFLEDGKRVI
                     SIGGEHLITYPLFLAHKEFYPEFTMVHFDAHADLREGYAGDELSHASVMKLCLDKGLE
                     RLIQIGIRSGTKEEYQLRQTDHRIIPANTLTEAADALEEGEVIYISIDLDYFDPSFVP
                     GTGTPEAGGGSFNDFMKFIKIINEKKCRIIGADIVELAPDLDSSKISIAFTAKVLREL
                     LISSHLNS"
     gene            7608..10913
                     /locus_tag="PL056_06370"
     CDS             7608..10913
                     /locus_tag="PL056_06370"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPM46673.1"
                     /translation="MKRAALLFSLIALSFVSCQDLADKFKKKVAENVPGIEKDIVKIE
                     ELVKDLASDGSNTISWLRYPSISPDGREIAFSFMGDIYITSVKGGYARPLTHASSFES
                     APVWSNDGKNIAFLSDRHGNHDVFIISVNGGEAKRLTHHSANDIPLTFSPDDSKIYFS
                     SARAGSKESSAFPTRGMPQLFSVSVNGGNDFLEIPLPVFDLRISKDGKRFVYHDKKGY
                     ENEWRKHHTSSVTRDIWEYLPEDVKFNKISNFSGEDRSPAFSPDETAVYYLSEKSGSF
                     NVWKIDSGKVETQITKFDTHPVRFLSASRDGTLCFSYHGDIYTMKEGSNPQKLEIFSP
                     KEIRKQDIRQMLQSVTDMAISPDGTEAALVMRGEIYVINPETGTTKRITQTPNEERWV
                     SFHPDGRKLLYSSFRNGSWNIYETVLEDENELYFFASTKMTEKPLIEGKFNTFQPGYS
                     PDGKKVAYLRERTELVVYDTEKNLHTTVLSGDRNISYVDGDQEFTWSPDSSRLAVVFL
                     DRDLWSGEIGIVSSDGRGPVVNITNSGSDDTNPKWSSNGEMISWFNHGEITAFFMNRS
                     GRDNFFMTKEEFDIKKKRDEKKKENEIKEKASSGNSKDKDEKKPDPVNFETEKIEKRI
                     VKLSLNPANYLDHTFSSDSETLYFMIMEPAEYKIISLSMREKKDKLIASIPRPRNLSY
                     WEKPEFSIILDKTGSSLFAVADNRTYKITVADGKMKPLAPAAELSVNFQDEKFYLFEH
                     VWKTVNDKFYVAEMHNVDWKKYFNEYVKFIPFINNNYDFTELLSEMLGELNASHTGSG
                     YIPQKPLGDDTGRLGIFFSYSQRGLIVDEVMTGSPLDKAGSRIKTGTVIEQIDGISTL
                     ENNFDSLLNRKAGRNVRLSLLDPETSNRWEEVVKPVPYRAEFDLLYTRWVEKNRERVE
                     ILSKGRLGYVHIRGMNGQSFKEIYNDILGRYNDREGIVVDTRFNGGGWLHNELSILFS
                     GKSYTRYSHRGVKNFGGDPNNQWTKKTVLVVNEGNYSDAHLFPHAYKTLKLGKIVGMP
                     VAGTSTAVWWPSMLDSTMYFGIPQIGIRDTDDNHLENSQLEPDYLVPVSPEQYIRGED
                     PQIEKAVKVLLEN"
     gene            complement(10910..>11282)
                     /locus_tag="PL056_06375"
     CDS             complement(10910..>11282)
                     /locus_tag="PL056_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008779366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HPM46674.1"
                     /translation="VKIATKGAQKEMILPAMIGFLTPAVVGTFVGIAGVMGYLLGIIS
                     TGLLMAIMMSNAGGAWDNAKKIIESGVHGGKKSEAHKAAVVGDTVGDPFKDTTGPSLN
                     ILIKLSAMSSIIFVGLALLKF"
BASE COUNT         3488 a         2352 c         2039 g         3303 t
ORIGIN      
        1 aacacaatct cgacatcatc aaatgctcag attacataat cgacatcggc ccacagggcg
       61 gagatgaagg gggaaaaatc gtggcagtcg gaactcccga agaagtcgca aaaaacaaaa
      121 aatcactcac cgggaaacac cttaaaacgg ttctttgaga tttaaacaga tatccccttt
      181 tccgtcacag taaaccgcat attcaccaaa catcttccta aaagtgataa cccccgcatc
      241 ctcaatgttt tcaaggtgtc gtctatagat tcgcatgagg taggcatcta agttctttaa
      301 tttttgctct aaatttatgt tatattttct ttgttttttg gcaattagga aatttcgaac
      361 aaccccagaa atagccccat tttacttttc tttttaacat cttagcacca caatcacatc
      421 ttggtattaa tgctttttct ttttctattt tttgcatttg ttcttttgta aacaatatgt
      481 aatcgtctgt tccttttccc catttatgca acagttctcc gcacttctca caagataaag
      541 aatcaccctc atcttttact ggatattttt tatgctctat aaaatactct gccccacaac
      601 ctttacaaat aacccaccct gtattcatgt ctttactctc taaagaaaag tgaagtgtta
      661 ttttcttttt ttatcagtat ttccatcaat acaaactagt gcactcctct acaaatcttt
      721 ttgaatgatt caagattctt tcttcgtttt ctttgtttag atcgtttgca cttaaagcag
      781 ataataattt aaggaattcc tcaaaacatt ctattgttaa attctgtcct gctttttcca
      841 aatttttcaa aaccttatca tcaatcatgc catacaaatc ctttccatgt tttttaagag
      901 ctgtgccaag gaaataaatc atatccgaga aatcaattgt cccataaagc ttagaataaa
      961 ttggtggaat taaatactct tctttttcat taaatactcc ataagaacca ttcactttta
     1021 ccaaatactt cccattttca ataggttcta tttcttcata tttcggctca aaaatgattt
     1081 caccattata atcaataagc ccgaatttat catctttgta aactacaaca tttttatttc
     1141 cccaataata aaactctaaa ccatgtttca gcgtttttat aattttgtga ttttcatcca
     1201 aaagatagat atcctctcct tttctagcga atgctcttcc atttgaaaat gaagatataa
     1261 aatcatataa tccagctggc aaaacctcct ctcctttttt attaagcaca ccagttttaa
     1321 aatctttctt gaaaaccaca aaattgcttt cagattttga agggatagtc aacgaatcat
     1381 atttgaaatc tataattgtg ttgccttcta aatcaatcac tccaagttta ccttcttttc
     1441 ttgcaactat gtatcttgac tcatcgaaat ttccatgtcc ttcatcaaaa acataatctg
     1501 taatcctgac atttttccta aataatgcat atttaccgtt tttcataaac ataaaatatt
     1561 cattagattt gggtatcgtt tctgctagtt catgtaaagg ttgtacaata aaatcacctt
     1621 ttcgatcaat tactccccac aaaccattaa tcatcacaac cgcagcgttt gcagaactgt
     1681 aaaacgaact tgcaaaatca aaaattggct tgattataaa atcacctctt ttgttaataa
     1741 aaccgacctt tccatcttcg tttgaagcgg gagccaaacc atcaaaaaag gtgccaagat
     1801 attccatttt aggctcaatt aaaatgtttc catttaaatc tattaaaccg tatttgttgt
     1861 ttactttgaa agacatcaca atttcatcat catcggatat taataatatc gttgcaacaa
     1921 ttattgtaaa aagaagaaag gccatgagaa aaacaacttt attgatgatg aattttcttg
     1981 tttttgtaac ttttgttgtt tcctctttct cttgaacgcc aatctgttta tcttttataa
     2041 acttatcttg tggtagtccg caagatggac atctttccgc atactcactt attttttttc
     2101 cacactctgg acaattaata atggccattt ccacaactcc tttttattgg attattctcc
     2161 tttttctgtt ttatgcgagc aataattgca cattccatga tcagaaatag catcatattt
     2221 ttctgaccat gatatatcat caccacatct tttgcattta attcctttat aaaaatcaac
     2281 aacttttctt ctaaaaacag ctttttgttt ttctgacaaa gactcttcac ctttagaaat
     2341 aacctgcttt gttattccaa gaacaggttg ttcaataaaa ccaccttcaa caatttgctg
     2401 gagaaaatcc ttaaatcctc tgtcttcgtg atcattgtgc cccattgtac tgcctctcct
     2461 ttaaaattat ttaatctcgt tcctatctct ccagaataat agattcagcc cctctttttg
     2521 tcaaaataaa gggaatattt attgtctctt ttttgaattc atcaaattat gatatctttt
     2581 ggagttattt aatgaaaccc gttgttagaa aaccctctgc tgttgaaaaa atcaaccctc
     2641 aatttcatca ggaaattcag taattctaat tttatttact gtttcctgaa gttgaggaat
     2701 gtcggtatga agagttttcc agagcacatg gatgtcaact ccgaaatatg catgaattaa
     2761 aacatttctc attccataga tgtctttcca tggtatttca ggatattttt cttttaactc
     2821 ttcaggaatt ttgttgactg cttcaccgat gatctcaaga gctcttgtta ctgcgaaaat
     2881 cgttttatca tctttgaaaa actgctcttc tgttatttcg gatataaatg actcaatctt
     2941 ttttatggca tctgaaatat cgtccagata taacaaagta tctcttatca tattgcaagt
     3001 gcctctttta acgctgtttt ggcaactctc ggtttcaatg aatctttaat gaccagatca
     3061 acttttattc ccaaaagatt ggaaagctct ctttgaactc ttataaattc aaaaagtgac
     3121 ggaacaacat caaattcaac aattaaatca acatcgcttt tgtcagtctg ctcgtttctg
     3181 gcataagaac cgaaaagata gattgattta attttaagag atctcaggaa atctttatta
     3241 accctgagca acgctatcac ttcagatgtg ttgatctttt tcgcaaccat ttccataact
     3301 cccgaaacat tcgaatccca ttttacacaa cttattctaa aaataaagaa ctgtctgaaa
     3361 ttatatacaa aacggttttg tgaaccagtt tcatcaatat ttaagctttc tcaggaaaaa
     3421 ctggataagt tcttcatttg attcatcaat tatgttcatt gcggggatgt agaaaatccc
     3481 ttgaagtcct ttcttttctg cttcttccat gagggctgat gcgtgataag gatggtggta
     3541 aagtgcgtat aagttagtcg gttttcctgc atcgtcataa gcgttattca caaatatttc
     3601 ggggtcatcg gcactcatga gatcaagcat atcaaccttt ttacgatatt cagtaatggc
     3661 cggagtatat atttcatcaa gtgaagagac tccgtaaaaa gagtaaagtg tcggcccgaa
     3721 cccaagattt acaaatgatt tcaaggtgat gtcatacggt ttgaaaattt ctgtctccca
     3781 cctcacaatg tcataggtcg cctgagtttc aagtgcggca acgcccttca accgcgttga
     3841 ttcacgaaga accggatcct cgctttcagg atcagccata tcatcattaa aaccgatcca
     3901 cagacttgct ccggcacctg ctgaaaagcc gtatgaaaca attttttcct tatcaatgtt
     3961 gaatacttct gattcactcc ttataaactg caacgccctt ttacagtcat tgagcggctt
     4021 tatcaccccg tctttatcat tctgttcaag gagctgataa ttcacacttg cgaatgaaat
     4081 tccgttttca agcagcgacc ttatcacaaa cggtccaccc gcttcatagg catcgctttt
     4141 atcttccatt gtgaaaccac ctccgtgaat ataaattaca agcggagccg gttcttttgt
     4201 tcttgcaata aaaatatcaa atgtattcaa ctgcttatcg ccataggcga tgtcagcatg
     4261 gaatcttgct tccacgccgt taagatctat cggtgaagga tcagaaggaa catcataaaa
     4321 cccgtcgtag tcatcttcat ccggctcttc ttccgactcc gttaaaactt ccgactccgc
     4381 caagtattcg tcggacaaat cgtcggacaa atcgtcgggt aagtcgtcgg ataagtcgtc
     4441 ttgatcaacg agatcgtcat cgctctcatc attgtcgggg atcataatgt cagaattatc
     4501 tgaatcgttt ttaacttcag cttcaggaac gactatcttc ctgaaattgt tatcacaccc
     4561 tgtcagaaca ataaaaacaa acagttgaac caaaatcagc tttttcatca agcaatctct
     4621 caaacagttg tcaacacaat atataacgga aaattgagta aaaaagtttt tatattactg
     4681 actgttcttg ataaatcctg ttttgccgat attatgaatc agttttgttg aaaattagaa
     4741 ctcttggaga ttttatgaac aaagctctgc ttatcgtgat cctgttattc tgtctgttta
     4801 atatctctgc atctgagcag agcgggaaag atgggtggac tttaaagaaa aaagaggacc
     4861 gttatcagat atatcagaaa aaaacaccgg gatctgactt taaagatgca aagaccatcg
     4921 ccctcataac cggatcgata gatgaatcct ttaaagttgt tacagaccct gcaaatttca
     4981 cagcactgtt caatccccat gtcgaaagat cggctgtttt gaaaaaaaag gaaaaatgca
     5041 atgagatcta cattctgata aatccgcctc ttttttcaag gagagaaact actgttgaag
     5101 tctgctattc agatctttcc gaaacatctt tcaagctcaa ctggaaaaac agtgacacaa
     5161 ataacagcgc caagagtgaa tatgtcagaa tgaacacttt ttcaggaaca tgcagtttct
     5221 caagaactga ggcgaacgat gagctcaaag ttgagtgcat cacctcttta gaccttggcg
     5281 gctcaatccc gatctttaca ataaacgaca taaattcaag agctgtcgtt caattcatct
     5341 ggaaaatata ccacgaaata cttaatcaaa ggaaaaataa agggggtcag aagtgaaaaa
     5401 gcttatcgtt tcagcatcaa tttttctttt tgccggcttt tttctttttg cggctgatga
     5461 tccggcacag aaagaagaag aaaaaaagga tgaaaaatct tcttccggct ggtttggtga
     5521 gccggagccg gcaatgcttg agcttggact cggttttttc taccacacat accattttga
     5581 aactcatgat gatgatggcc gtgatgaaac tcaaataaat taccgtaaag atctcggata
     5641 tgaaaaggca tatcttcatt ttgatgcaga ttttgctttc agattcaaaa aattcaagct
     5701 ttcagccgga tacacgatgc tcaatgcagt ttccaaaaaa gcagcaggaa gagaactgac
     5761 tttttatgat gcaatcttca acatgggtga agatatgaaa agcactttca atgtccacct
     5821 gataaaactt gatttcacct ggtatattct tgatcttgat gtcggtaaga accttaattt
     5881 caagctcggt ccttcgatca ggatagatat gtttgtcacc gatatccgtc ttgaaagtac
     5941 cgaagacaaa agcaagaaag acaagtttat ctcacctgtt gtccccttcc cttctctcgg
     6001 tatttcattt gaaacaacaa tatttaaata ttcagggatt ttctttgata tcaacggaat
     6061 gtacgcagga aaatatctcg gtttcatgga tctgaaatca gggatcagag tttatccgtg
     6121 gcactggaca gggatcgaat ttgcatacag acaccttctt gcgaaggcac tctggcaagg
     6181 tgacggagta gagaccgagt ttaagggttt caacatgagc tttattttaa gattctgaaa
     6241 tcattaatga gttgtccgct tatctcagca ataatcccgg tttttaacag acctgaactg
     6301 gtaaaaagag caatcaactc catcttgagc cagacttata aaaactttga gataattgtc
     6361 gtcgatgacg gttcaaccga tgaaactccc gttgttctca aaggttatga cggaaggata
     6421 aacgcaatca gaactgaaaa taaaggtgta agtgcagcaa ggaacactgc cgttaannnn
     6481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaaat atacgcaaac ggagccagaa
     6601 aacggggaaa cgacgaaaac gccttgtggg cagaaaacat cgtgaaagga tcataattct
     6661 tgctttttgt gactctcacc gtatttttgt tgaagtatct gttttttata ttaattcaag
     6721 gagaaaacca tgtttcagat cccgcggttc atttcttcaa gggaaaatcc tgaggaagca
     6781 tcgataattg ttgccggaat tccctatgac tgcacttcat ctttccgttc tgcaagccgt
     6841 ttcggcccga gagaattaag aaactatgca tttgaagcaa ttgaagagtt ctcgttctat
     6901 cagggaaaaa ctctcgacga tgtgccgttt tgcgatgtcg gagatcttga agtgatggtg
     6961 ggaaaccctg caaacatggt aaaaaccgta atggaagaag tcaaaccttt tctcgaagac
     7021 ggcaaaaggg tcatttcaat aggcggagaa catcttataa catatcctct ttttcttgca
     7081 cataaagagt tctatcctga attcacaatg gtccattttg atgcacacgc cgatctccgt
     7141 gaaggttatg caggagacga acttagccat gcatcggtca tgaaactctg ccttgacaaa
     7201 ggacttgaac ggttgatcca gatcgggata agaagcggaa caaaagagga atatcagctc
     7261 cgtcagaccg accacagaat catccctgca aacactttga ctgaagcggc tgatgctctt
     7321 gaagaaggtg aagtgatcta tatttcaatc gaccttgact actttgatcc ttcatttgta
     7381 ccgggaacgg gaacacccga agcaggcggc ggatcattca acgacttcat gaaattcata
     7441 aagataatca acgaaaaaaa atgcagaata atcggagcag acattgttga acttgctccc
     7501 gaccttgaca gctcaaagat ctctatcgct tttacagcca aggttttaag agagctcctt
     7561 atcagttcac accttaactc ttaaagtttt taccggagga aaatattatg aaaagagctg
     7621 ctttgctatt ttcccttatc gcactctctt ttgtttcgtg tcaggacctt gcagacaaat
     7681 tcaagaagaa agttgcagaa aatgttcctg ggatcgaaaa agatatcgtc aaaatagaag
     7741 aactggtcaa agatctggct tctgacggtt caaataccat aagctggctg agatatcctt
     7801 caatatcacc tgatggtcgc gaaatagcat tttcattcat gggtgacatc tatatcactt
     7861 ctgtcaaagg aggatatgcc agaccgctca cccatgcttc aagctttgaa tcagctcctg
     7921 tctggtcaaa tgacggaaag aacatcgcat ttttaagcga ccgtcatggg aatcacgatg
     7981 ttttcatcat ctctgttaac ggcggagagg caaaacggct cactcaccac tcggcaaacg
     8041 acattccgct caccttttca cctgacgatt caaagattta tttctcttcg gcaagggcgg
     8101 gcagtaagga aagctcggct ttcccgacaa gagggatgcc tcagctcttt tccgtttcag
     8161 taaacggcgg gaacgatttt cttgagatac ctcttccggt tttcgatctg cgtatttcaa
     8221 aagacggaaa aagatttgta tatcacgaca agaaaggtta tgagaacgaa tggagaaaac
     8281 accatacatc aagcgtgaca agagatatct gggaatatct gcctgaagat gtaaagttca
     8341 acaaaatcag taatttctca ggagaagacc ggtctccggc attttctccc gatgaaacag
     8401 cggtatatta cttaagtgaa aagagcggct cgttcaatgt ctggaaaatt gacagcggca
     8461 aagttgaaac ccagatcaca aaatttgata cccaccctgt aagattcctt tccgcaagcc
     8521 gtgacggaac actctgtttt tcataccatg gagatattta cactatgaaa gagggttcaa
     8581 atcctcagaa acttgagatc ttttctccaa aagagatcag aaaacaagat atcaggcaga
     8641 tgcttcaaag tgtcaccgat atggccatca gccctgacgg aactgaagcc gcccttgtta
     8701 tgcggggtga aatttatgtt atcaatcccg aaacaggaac aacaaaaagg atcactcaaa
     8761 ccccgaacga ggaaagatgg gtaagcttcc atccggacgg aagaaaactt ctttattcat
     8821 ctttcaggaa cggttcatgg aatatatacg aaacagtgct tgaagatgaa aatgaactct
     8881 atttcttcgc ttcgacaaaa atgacagaga aaccgctgat cgagggcaaa ttcaacacct
     8941 ttcagcccgg ctattcacct gatgggaaga aagtggcata tctcagggaa agaaccgaac
     9001 ttgttgtata tgacactgaa aaaaaccttc acacgaccgt tttaagtggt gacagaaaca
     9061 taagttatgt ggacggtgat caggaattca catggtctcc ggacagttcc cggctggctg
     9121 ttgtatttct tgacagagat ctctggagcg gagagatcgg catagtcagc agtgacggta
     9181 gaggaccagt cgtgaacata acaaactccg gctcagatga cacaaacccc aagtggagca
     9241 gtaacggtga aatgatctcc tggttcaatc atggtgagat aacagctttt tttatgaata
     9301 gatccgggcg tgacaacttt ttcatgacaa aagaggagtt cgacattaaa aagaaaaggg
     9361 atgaaaagaa aaaggaaaat gaaataaagg aaaaggcatc ttccggtaat tcgaaagata
     9421 aagacgaaaa gaaaccggac cctgtaaact tcgaaacaga aaagatcgaa aaaaggatcg
     9481 ttaagctctc cttaaacccc gcaaactatc ttgaccacac attttcaagc gacagcgaga
     9541 ctctatattt catgataatg gaacctgctg aatataagat aatctcactt tccatgaggg
     9601 aaaaaaagga caagctgata gcttcgatcc cccgcccgag aaacctttca tactgggaaa
     9661 aacctgaatt ttcaataatt ctggacaaaa ccggttcttc cctctttgca gttgccgaca
     9721 acaggacata caaaataact gtggccgatg gaaaaatgaa accccttgca cctgccgctg
     9781 agctttcggt caactttcag gatgagaaat tttatctctt cgaacatgtc tggaaaacgg
     9841 taaacgacaa gttctatgtt gcagaaatgc acaatgtcga ctggaagaaa tattttaatg
     9901 aatatgtgaa gttcatccct ttcataaata acaactatga tttcacagag cttctgtccg
     9961 aaatgctcgg tgaactgaac gcaagccaca caggaagcgg atatattcct caaaaaccac
    10021 tcggtgatga taccggaaga ctgggcattt tcttttcata cagccaaaga ggactgatcg
    10081 ttgacgaggt catgacgggt tctcctcttg acaaggcggg aagccgcatc aagacaggaa
    10141 ctgtgataga gcagattgac ggaatttcaa cacttgaaaa caattttgat tctctgctga
    10201 atagaaaagc tggcagaaat gtacggctgt cgcttcttga tcccgaaaca tcgaacagat
    10261 gggaggaagt tgttaaacca gtgccgtaca gagctgaatt tgatcttctt tatacccgct
    10321 gggtcgaaaa gaaccgtgaa agagtcgaaa tactttcaaa aggacggctc ggctatgtcc
    10381 acatcagagg aatgaacgga cagagtttta aagagatcta caacgacatt ctgggcagat
    10441 ataacgaccg cgaaggaatt gtcgttgaca ccagatttaa cggtggcggc tggcttcata
    10501 atgaactgag tatccttttc agcggaaaat catacaccag atattcccac cgcggagtta
    10561 aaaacttcgg cggcgatccg aacaaccagt ggacaaagaa gaccgtgctt gtagtaaatg
    10621 aaggtaacta cagtgatgca cacctttttc cccatgcata caaaacactt aaattaggta
    10681 agatcgtcgg tatgcccgtt gccggaacat caactgctgt ctggtggcct tcaatgcttg
    10741 actcaacgat gtatttcgga attccgcaga taggcatcag agatactgat gacaaccatc
    10801 ttgagaacag tcagcttgag ccggactatc ttgttcctgt gtcacctgaa cagtatatca
    10861 gaggtgaaga tccccagata gaaaaagctg tgaaagtcct tcttgaaaat tagaatttaa
    10921 gaagtgcaag acccacgaaa ataatcgaag acatcgcaga aagcttgata aggatgttca
    10981 gtgacggacc agttgtatct ttgaaaggat caccgaccgt gtcaccgact acagctgctt
    11041 tatgagcttc agactttttg ccgccgtgta ctcctgattc aattattttc ttcgcattgt
    11101 cccatgctcc gccggcattt gacatcatta tcgccataag aagaccggtt gaaataatac
    11161 cgagaagata tcccattaca ccggcgatac cgacaaatgt tccaaccact gcaggtgtaa
    11221 ggaaaccgat cattgcaggg agtatcatct ctttctgtgc ccctttggtc gcaattttaa
    11281 ca
//
LOCUS       DAOOYB010000082        11177 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_6216_length_11177_cov_12.418540, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000082 DAOOYB010000000
VERSION     DAOOYB010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11177
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6216_length_11177_cov_12.418540"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /gene="secG"
                     /locus_tag="PL056_06380"
     CDS             complement(<1..359)
                     /gene="secG"
                     /locus_tag="PL056_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020888254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HPM46675.1"
                     /translation="MFETFAVIQIIICLLLVVVILLQEGKKGMGAIFGGSSSSIFGAR
                     GAGNILTKVTSVLAILFMLNSVWMSYISSRDASVIDEIKTTEESREPSMEPAPVKEEK
                     VEEPVKEEAPANKEEAPA"
     gene            506..1375
                     /locus_tag="PL056_06385"
     CDS             506..1375
                     /locus_tag="PL056_06385"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HPM46676.1"
                     /translation="MKKYFGGIDLGGTKILTVVTDENRNTLAKVKVATESVKGVEHVI
                     GNMFSSLEQCCNECRISISDLEGVGIGVPGPVDFEKGMVYDCPNLYGWKNIEVREIFK
                     RKFNIPVVLENDARVAGLAEVRLGSAKGFRHVFYVTVSTGIGGAMIFDRKIYHGADGA
                     AGEFGQMRLIDGTIFEQRYAGPAIERIFGISTHQIPELIKKNDPGAKKALEHLITGLG
                     TYLSNITTLLNPEIIVMGGGVSSIGDILLKPIEQEIRKQGFSISSKNVKVVRAGLGTD
                     SGAIGAVELLFDD"
     gene            complement(1372..2835)
                     /gene="thyX"
                     /locus_tag="PL056_06390"
     CDS             complement(1372..2835)
                     /gene="thyX"
                     /locus_tag="PL056_06390"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR02170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HPM46677.1"
                     /translation="MKVVLAGYNIDAQIIEELKKRASWSEDNVTPETLSAAYARISRD
                     PADIGSLRQKSRIETEKARKSNEMIIFGLGHSSVAEHAVFNFDITGLSRLAVEEVQKF
                     RLASFTEKSQRYIKLDGDFVMAEELHELHLSGKLEKLINIQNRTYFSLYETLRNHFFA
                     KFPSRITTKNGKITVDGWAKEDARYVVSLATVTQFGMTVNARTLENMLRRFASSDLQE
                     IRELGKRIFEAVHPLSPSIIKYTKPTSYDTEREKTIKRALEKYNERILDPQYDQIPVK
                     LHSFTKKPDEVLCAAFIYTYTGSDFQSCIRSASSLTNTQKKELIKASLCSKDSYDKVE
                     RAFESVEFTFELIVSATNYAQLKRHRMSSQIPQDYDTVLGYTVPPSVIETGQTALFSE
                     IMKRSEEFYNILRTTAPLSKNYALTNAHRRRVLMKLNARELYHFVSLRDDEHAQWDIR
                     ETAKEMKRLAEEAAPLTTMMLSGKSDFEECRKKLFKY"
     gene            2977..3534
                     /locus_tag="PL056_06395"
     CDS             2977..3534
                     /locus_tag="PL056_06395"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPM46678.1"
                     /translation="MVERDRGTKTIMSHGIKNEIIENTIVLIATKGSWSISVQEIADA
                     CKTTPASLYTYFGSKKEIFEEAREEMEKRFDSVISLPIPEKIPEEMKIRMISFYILDF
                     VTRNKWASDFIDPFSGCNATKKLMERISVLLKKNKLSKDELEYRIYRFLAGIHFKIKY
                     RFMRNEVPVEADIDDISKFMLYSEV"
     gene            3531..4205
                     /locus_tag="PL056_06400"
     CDS             3531..4205
                     /locus_tag="PL056_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46679.1"
                     /translation="MIKKALLFSIITALFSGLSADGSVIEKMYQKLSGFYEKNQGVIS
                     VRELSAVSKDPETGKVLKKFSAKIERTDYFYKTPVIKALQYTENGEENSVKNYDTREI
                     EPVYPVFDKKGKDNYTLEILGNENVDGRNCLKIKVVPRKISMRHFTGEIFIDPGKLEI
                     VKMKGSPSKLHWAMKEFSFEYLFAALDGFPVVKSAKVKARVKVALFVSDNITDYEVEA
                     LSNRFF"
     gene            complement(4227..5129)
                     /locus_tag="PL056_06405"
     CDS             complement(4227..5129)
                     /locus_tag="PL056_06405"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HPM46680.1"
                     /translation="MFSDSKFIEQFLDNLFEAVYFVDTSRQIMHWNKAAENLTGWKKE
                     EIIGHRCMDNILVHIDSEGNNLCTGNCPLIQSMKSRSLHQSDIYLHHKDGHRIPVHVR
                     IIPLFSESGESTGAIEIFSNTSADNDLMGQIDELRKLSMIDALTGIANRRYGEKIILD
                     RIDEKNRFGWDTGIIFFDIDNFKYFNDSFSHNTGDRVLKMVSNTLRESIRSFDHVSRW
                     GGEEFIVVLSNVTIEQLKEKAELLRRLTSESFFFEKEIRLFATISGGATMIRADDTVE
                     TVVERADHLMYQSKRSGKNKVTAG"
     gene            5259..8258
                     /locus_tag="PL056_06410"
     CDS             5259..8258
                     /locus_tag="PL056_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46681.1"
                     /translation="MFTFYRIFISVAENITDIINDSLKSHSQKIEEFMLSAEKFREAI
                     FVKGENVDLKILWDDIVTHIDDLVALSEKHVNTDLKLGPVVDGIYQNFSADIDALSEK
                     EMVWFLLGFKNGIIKDLESSGTVKLFLHNQYHFDCYRHTFKIDEVLKRYFQNSFLSEF
                     MYIAEKHICRAVLLEAEHFSKLHEKFEEFQDDMMFFDEFPQTVSKKRVEVPPVLERVD
                     RYIAGFATFFSEIEKVKKEIHGALMEAVDKALHSAVGKAYMLNSPVVRSLFYSVRKVE
                     SNKEKIREKFLSDDRMFSDHFSIEISDWKKDLEISKYQIFTGDLTVDTVNLSDNKLEE
                     RYRPAFLQINKMLESTINVISSFQPGDFQKIKMAILKESRAIQKALREEHVPGIIDVA
                     QKASILSVIKNFVQRIDDAIEKIDVSHTIFSSKKVSNNVTVSKSINVPLRELIRYKTD
                     SALVVQLADFIDDIRKNNEKVIRKTGEIEKIVEYNMDAALSVMRNRDEEDEEAAIKAV
                     TIAKEGLERALNLSNELLGIIMLTVEKIRTVMPEIVSEYLNQIEELTFAEKVIKLNIY
                     LVRKRTVKNLKEHLIAIKAFSEKTLPELINLVAEYFNKTKKNYSKFKKITGLSQSKDD
                     FGFFYMSRLNEMENRFRSMPYVYQRLFSYDSLPDRNFYIERKNVAEKLLEDFTYWKSG
                     GFVSTVLVSEKGNGKTTFFDIARAELLKEHRIYKAELLKTVRTSSELLEVLKDSFEMT
                     DASSIDELEQRIMSNDSRKIFFLENLQNMYLRTMDGFEAVERFLLFMERTQEKVFWIV
                     SCNLYAWNYIDLAVSASRYFQKTIVISSLSSIEIEELIMKRHNSSGYDILFEVPEEIR
                     TAKKFSKIKNVDDRQNYLKKLYFEKLAKLSGGNIKSSILMWLSSIRDVAQDRMTVSAD
                     LLTDYPFIIDLPKEEKFALTTLIIHDALDPDDYSYVSRENKSGSSMMLLRLFNKGLLV
                     RKDENFQIHPFIYRDVVRVLKSLNMLH"
     gene            8268..9284
                     /locus_tag="PL056_06415"
     CDS             8268..9284
                     /locus_tag="PL056_06415"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HPM46682.1"
                     /translation="MKSIFTVLSFIIAINAGNSENCQSIDKRYVEEDFFNIDKVSFAE
                     ELEKPSETQSEKLPESDDAENKNPVSGTIIDSKPQIEWKVSFGKVFWMIIIFLFAYML
                     IKYFTKALEAIAEKWAASRLFIKRVIPVFRLSGWIATIYLMIVGVLAPPIETILAVTA
                     SFGIAMGFASQDILKNIFGGIMILFDNPFQVGDKIQVGEHYGEVIHIGLRSVRIVTPD
                     DSTVSIPNSDIVSRSVSNSNTGEFNCQVVAEFYFPIDVDLEKLRKLATRAAIVSKYVY
                     LRKPVAVRFKNEIHEGRSMVKMRLKAYVLDLRYEFAFSTEMSEIVISEVLKQGLVTPQ
                     QMSG"
     gene            9309..10472
                     /gene="chrA"
                     /locus_tag="PL056_06420"
     CDS             9309..10472
                     /gene="chrA"
                     /locus_tag="PL056_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013362680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HPM46683.1"
                     /translation="MKYGSFLKDVFICSLGAYGGPEAHFSIFLDQMVVKKKYLTEEEL
                     VELVALCSILPGPTSTQALVSIGYKNGGPLLGFLTMIVWALPVLMVMTALSFLYQFLS
                     FQGISSGVLRFIGPMAVGFIIVAAFRIGRKVVKDIPTGALMLFGAVTTFFIRAPWIFP
                     VVLLIGGAVTIFLSREKGIWNKIKLAPPWGYLFVFAVIAVLAIVLATVTDIRMFQLFE
                     SFYRYGYLVFGGGQVIVPVMHSELVEVRQYITNEEFLTGYGLVQGLPGPMFSFTAYAG
                     GMAARDSGIFVQIMSAAVSGIGIFLPGVLLIYFVYPVWEELKQIKAVKISLTGINAVA
                     GGLIAVAAVILMKASGFQIENLIVTAVSVALLMSRKVPAPLIVVAAILGGVFV"
     gene            10606..>11177
                     /locus_tag="PL056_06425"
     CDS             10606..>11177
                     /locus_tag="PL056_06425"
                     /inference="COORDINATES: protein motif:HMM:NF022018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HPM46684.1"
                     /translation="MTENELINIDDIKSRIFTIRGMQVMLDSDLAEFFGVETKRLNEQ
                     VRRNILRFPEEFCFKLSKEEKNKVVAICDHLQNLKYSHHLPSVFTEQGVAMASALINS
                     DIAVMMSIKIMKAFVAMRRFISNNGDVFKRMETVEIKLLETDSKIEKVLSALESKQIQ
                     PKQGIFFEGQVFEAHKFVSDIVRSAEKSIIL"
BASE COUNT         3385 a         1997 c         2437 g         3358 t
ORIGIN      
        1 gccggggctt cttctttgtt tgccggggcc tcttctttaa cgggctcttc gaccttttcc
       61 tcttttaccg gagccggttc cattgaaggt tccctgctct cttcagtagt tttgatctca
      121 tcaataacag atgcgtctct gcttgagatg tatgacatcc agacagagtt gagcatgaaa
      181 agtattgcaa ggaccgaagt aacctttgtc aatatattac cggctcctct tgctccgaaa
      241 atggaactgc tggaaccgcc gaaaatggct cccattcctt ttttaccttc ctgaagaagg
      301 ataacgacaa cgagcagaag acagatgatg atctgaataa ctgcaaaagt ttcaaacatt
      361 ttgaacctca tacaagttta aaaaaccgta agcgtgcaat ttatagccga ttcggtttta
      421 ttgtcaaact ttttctgtca tattctggaa aagtgccgaa aatacagtac aatggcttcg
      481 cgtaatcttg taacaggagg ataacttgaa aaaatatttc ggaggcatcg accttggcgg
      541 aaccaaaatt ctgacagttg taactgacga aaatagaaac acacttgcca aggtaaaggt
      601 tgcaactgaa tcggttaaag gtgttgagca tgtgatagga aatatgtttt catctcttga
      661 acagtgctgc aatgaatgca ggataagtat ttcagatctt gaaggagtgg gaatcggtgt
      721 tccggggccg gttgattttg aaaagggaat ggtatacgac tgtcctaatc tttatggctg
      781 gaagaatata gaagtccgcg aaatatttaa gagaaaattc aacatccctg tcgttttaga
      841 aaatgacgca agggttgccg ggcttgctga agtgcggctc ggaagtgcaa aaggtttcag
      901 acatgttttt tatgttacgg tcagcacagg cataggcggc gcaatgatct ttgacaggaa
      961 aatatatcac ggagcggatg gagcggcagg ggagttcggg cagatgcggc ttattgacgg
     1021 aacgatcttt gagcagcggt acgcgggtcc ggcaattgaa agaatatttg gcatttcaac
     1081 tcatcaaata cctgaactta ttaagaaaaa tgatccggga gcaaaaaagg cgcttgagca
     1141 cctcatcacc ggacttggaa catacctttc aaacataacg acattgctca accctgaaat
     1201 aattgtaatg ggaggaggcg tttcgtccat cggcgacata cttcttaaac cgatagaaca
     1261 ggagattcga aaacaggggt tttccataag ttcaaaaaat gtcaaagttg tcagagcagg
     1321 tcttggaacc gattccggag caataggggc cgttgagctt ttgttcgatg attaatattt
     1381 aaaaagtttt tttctgcatt cttcaaaatc gctcttgcca ctgagcatca tggttgtcag
     1441 aggcgcggct tcttctgcca gccgtttcat ttccttagca gtttcacgga tgtcccactg
     1501 agcatgttca tcatcacgga gactgacgaa atgatagagt tctctggcat tcagtttcat
     1561 caggactctt cttctgtggg cattggtcag tgcatagttc tttgataaag gggctgttgt
     1621 tctgaggatg ttataaaatt cttcacttct tttcatgatc tcggaaaaaa gggcggtctg
     1681 tccggtttca attacagacg gaggaacggt atatcccaga acggtatcgt aatcctgcgg
     1741 gatctgggag ctcatacggt gtcttttaag ctgtgcataa tttgtcgcac tgacgatgag
     1801 ttcaaatgtg aactctactg attcaaaagc gcgctcaacc ttgtcatagc tatcttttga
     1861 gcacaatgat gcttttatca gttccttttt ctgagtattg gtaagcgatg aagcggatct
     1921 gatgcacgat tgaaagtcag atccggtgta agtgtaaata aatgccgcac agagaacttc
     1981 atcgggtttt tttgtgaagc tgtgaagttt tacagggatc tgatcatatt gaggatcaag
     2041 tattctttcg ttatattttt ccagagcacg ctttattgtt ttttcccttt cggtatcata
     2101 acttgtcggt tttgtgtact tgattattga tggtgagagt ggatgaacag cttcaaatat
     2161 tcttttcccg agttcccgta tttcctgaag atctgacgat gcaaatctgc gaagcatgtt
     2221 ttcaagagtt cttgcgttca cggtcattcc gaactgtgtg actgttgcaa gtgatactac
     2281 atatcttgcg tcttcctttg cccagccgtc aacggttatt ttgccatttt ttgttgttat
     2341 tctggacgga aattttgcaa aaaaatggtt tctcaatgtt tcataaagtg agaaataagt
     2401 acggttctgg atgtttatga gcttttcaag ttttcctgaa agatggagtt catgaagttc
     2461 ttcagccatg acaaaatcac cgtcgagttt tatgtaacgc tgactttttt ctgtaaacga
     2521 tgcaagccgg aatttttgaa cttcttcaac tgcaaggcga ctgagtccgg taatgtcaaa
     2581 attgaaaacg gcgtgttccg ccacacttga atgaccaagt ccgaagatta tcatttcatt
     2641 ggatttccgg gctttttctg tttcgatgcg ggatttttgt cttaaactgc cgatgtctgc
     2701 cgggtctctt gatatccttg catatgctgc actcagagtt tcaggggtga cattatcttc
     2761 actccacgaa gctcttttct taagttcctc aatgatctgt gcgtcaatgt tgtatcccgc
     2821 aagaactact ttcatgtctg ctccattcaa attgttccgg acggcattat aaaaaatgaa
     2881 gtgtcttaaa tcaactttgt ttttttaagt aaatttgtaa aagatcttaa aagggctata
     2941 attaaacagt ttacaacctt tcggaagaaa atgtaaatgg ttgaaagaga tcgcggcacc
     3001 aaaacaataa tgtctcatgg aataaagaat gaaattattg aaaatacgat tgtcctgatc
     3061 gcgacaaaag gcagttggag catatcggta caggaaatag ccgatgcctg caaaacgaca
     3121 ccggcatcgc tttataccta tttcggatct aagaaagaaa tatttgaaga ggcaagggag
     3181 gagatggaaa aaaggtttga ttcagttatc agtctgccga tacctgaaaa gatacctgaa
     3241 gagatgaaaa ttagaatgat atctttttat atacttgatt ttgttaccag aaacaagtgg
     3301 gcttccgatt ttatagatcc tttttcaggg tgcaatgcca caaaaaaact tatggaaagg
     3361 atcagtgttc tgctgaaaaa gaacaagctt tcaaaagatg aactggaata caggatctac
     3421 agatttctcg caggtatcca tttcaagata aaataccgtt tcatgcgtaa cgaagtccct
     3481 gttgaagccg atatagatga catttcaaag ttcatgcttt atagcgaagt atgattaaaa
     3541 aggcacttct tttctcaatt attacagctt tattttccgg actttctgct gatggatcag
     3601 tcattgagaa aatgtatcag aaactgtcag gattttatga aaagaaccaa ggtgtcataa
     3661 gtgtaaggga attgagtgca gtttcaaagg accctgaaac aggaaaagtt ttaaaaaaat
     3721 tctcagcaaa aattgaaagg actgactatt tttataaaac gcctgtgata aaagcgcttc
     3781 aatacactga aaacggagag gaaaatagtg tcaaaaacta tgacacaagg gaaattgagc
     3841 cggtataccc ggtctttgat aaaaaaggaa aagataatta cacgcttgaa atactcggca
     3901 acgaaaatgt tgatggacgc aactgcttga aaataaaggt tgttcctcga aaaatatcaa
     3961 tgaggcattt caccggtgag atattcatag accccggaaa acttgaaatc gtcaaaatga
     4021 aaggatctcc ttcaaaactt cactgggcaa tgaaggaatt cagctttgaa tatctttttg
     4081 ccgcacttga cggctttccg gtcgtaaaat cggcaaaagt gaaagcaagg gtcaaagtcg
     4141 ctcttttcgt atctgataac atcaccgatt acgaagttga agcattgtca aacaggtttt
     4201 tctgatttta cattgtactg taatgttcac cctgcggtga ctttgttctt tcccgatctt
     4261 tttgactgat acatgaggtg atctgctctt tcaactacag tttcaacggt gtcatcagca
     4321 cggatcattg ttgctccgcc ggatattgtg gcaaaaaggc gtatctcttt ttcaaagaag
     4381 aatgattctg atgtaagccg ccgtaaaagt tcggcttttt ctttcagctg ttcaattgtc
     4441 acattgctta agacaacaat aaattcttct cctccccatc ttgaaacatg gtcaaagctt
     4501 cttatgcttt cgcgaagagt gtttgaaacc atcttgagca ctctgtctcc cgtgttatga
     4561 gagaatgagt cgttgaaata cttaaagttg tcaatatcaa aaaatatgat ccctgtgtcc
     4621 catccgaacc tgttcttttc atcaattctg tcgaggatga tcttttctcc gtatcttctg
     4681 tttgcaattc cggtcaaggc atcaatcatt gaaagttttc tgagttcatc tatctgaccc
     4741 ataagatcat tatctgcgga tgtgttggaa aaaatctcga ttgcgccggt tgattcccct
     4801 gactcgctga aaagaggaat tattctgaca tgtaccggaa ttctgtgtcc gtccttgtga
     4861 tgaagataaa tgtcagactg gtgaagggat ctgcttttca ttgattgaat aagagggcag
     4921 ttgcctgtgc agagattgtt tccttcgctg tcgatatgaa ccagaatgtt gtccatgcac
     4981 ctgtgtccga ttatctcttc ctttttccag ccggtgagat tttcagccgc tttattccag
     5041 tgcattatct gccgggaggt atcaacgaaa tagactgcct caaaaaggtt atcaagaaac
     5101 tgttcaatga atttactgtc agaaaacata aaaaacctct taaaaacaga aaagcaactg
     5161 attataacaa cacatatcgc attgtccata cataaatttg attttaatat caggaaatta
     5221 taaaatcacc agtttattta ataggcgaga ggttgtatat gttcactttt tacagaatat
     5281 tcataagtgt agctgagaat attactgata tcataaacga ttcacttaaa agccattctc
     5341 aaaaaattga agaattcatg ctttcagcag aaaaattccg tgaagctatc tttgtaaaag
     5401 gggagaatgt tgatcttaaa attctttggg atgatatagt aactcatatt gatgatcttg
     5461 tagcgctttc agagaaacat gtgaacactg atcttaagct cggtcctgtg gttgatggaa
     5521 tatatcagaa tttctcagca gacattgatg cattgagtga aaaagagatg gtatggttcc
     5581 tgctcggttt taagaatgga atcataaaag atcttgaatc atcggggact gtaaaactgt
     5641 ttctccataa tcagtatcat tttgactgtt accgccacac atttaaaata gacgaagttc
     5701 tcaaaaggta tttccagaac agttttctta gtgaattcat gtatatagcc gaaaaacaca
     5761 tttgcagggc agttctgctt gaggcagagc atttttcgaa actgcatgag aaatttgaag
     5821 aatttcagga tgatatgatg ttctttgatg aatttccgca gacggtttca aaaaagagag
     5881 tcgaagttcc tcccgtactt gaaagagtgg atagatatat tgccggattc gccacttttt
     5941 tcagtgagat cgaaaaagta aaaaaggaga tccatggagc tttaatggaa gcggttgaca
     6001 aagctctgca cagtgccgtt ggaaaagcat acatgctgaa cagtccggtt gtcagatcgt
     6061 tattttacag cgtcaggaag gttgagtcca ataaggaaaa gataagggaa aaatttcttt
     6121 ccgacgacag aatgttcagc gatcatttct ctattgagat cagtgactgg aaaaaggacc
     6181 tggagatatc aaaataccag atatttacgg gagatttgac tgttgatacg gtcaatcttt
     6241 ccgataataa acttgaagag agatacaggc cggcgtttct gcagataaat aaaatgcttg
     6301 aaagcacgat caatgtgatc tcatctttcc agccgggtga ttttcagaaa ataaaaatgg
     6361 caatacttaa ggaatcaagg gcaattcaga aagcattgag agaggaacat gttccgggaa
     6421 ttatagatgt tgcacagaaa gcgagcattc tttcggtaat aaaaaacttt gtacagagga
     6481 ttgatgacgc gatagaaaaa atagatgtca gtcacaccat tttcagttca aaaaaagttt
     6541 ctaacaatgt cacagtatca aaaagtatta atgttccgct cagagaactt atcagataca
     6601 agactgattc agcacttgtt gtgcagttag ctgactttat tgatgatata agaaagaaca
     6661 acgaaaaggt cataagaaag accggcgaga tagagaagat cgttgaatat aatatggatg
     6721 ccgccctttc tgtgatgaga aacagggatg aagaagatga agaagcagca ataaaagcag
     6781 taacgattgc aaaggaagga cttgaaagag cgctcaatct ttcaaatgaa ctgctgggaa
     6841 taataatgct gactgttgaa aaaataagaa cagttatgcc tgagatcgtt tctgaatatt
     6901 taaatcagat agaagagctt acttttgctg aaaaagtgat taaactgaac atatatctgg
     6961 taagaaagag aactgttaaa aatcttaaag aacatctgat cgcaattaaa gcattttctg
     7021 aaaagacact gcctgaactt ataaatctgg tcgctgagta ttttaataaa acaaaaaaga
     7081 attattcaaa gtttaaaaaa ataacagggc tgtctcagtc gaaagatgac ttcggctttt
     7141 tttatatgag ccgtctgaat gaaatggaga acaggttcag atcgatgccg tatgtttatc
     7201 agcggctttt cagctatgat tcacttcccg acagaaattt ttatattgaa agaaaaaatg
     7261 tggctgaaaa acttcttgag gatttcacat actggaagag tggaggtttt gtctccacgg
     7321 tgcttgtgtc tgaaaaaggt aacgggaaga ccactttttt tgatattgcg agagcagagt
     7381 tgctgaaaga gcacagaata tacaaggccg agcttttaaa gacagttcga acatcttctg
     7441 agctgcttga agttcttaag gattcttttg aaatgactga tgcctcaagt attgatgaac
     7501 tcgaacaaag gataatgtca aatgattcaa gaaaaatttt ctttcttgag aatttgcaga
     7561 atatgtactt gagaacaatg gacggatttg aagcggtgga aagattcctt ctttttatgg
     7621 agcgcacaca ggagaaagtg ttctggatag tttcgtgcaa tctttatgca tggaactata
     7681 tagatctggc tgtttcagct tcaagatatt tccagaaaac cattgtaata tcaagtcttt
     7741 ccagtattga aattgaagaa ttgataatga aacgacacaa ttcttcaggg tacgatattc
     7801 tttttgaagt tcctgaagaa ataaggacgg caaagaagtt ctcaaagata aaaaatgttg
     7861 atgacaggca gaattacctt aaaaaacttt attttgaaaa gttggcaaaa ctttcaggcg
     7921 gtaatatcaa atcatcaatt ctcatgtggt tgagttccat cagagatgta gcccaggaca
     7981 gaatgacagt ttctgcagac ctgttgactg actatccttt tattatcgac ctgccgaaag
     8041 aggaaaaatt tgcactgacc acccttatca tacacgacgc gcttgacccc gatgattatt
     8101 catatgtttc aagagagaat aaaagcggaa gcagcatgat gcttctgcgt cttttcaata
     8161 aagggcttct tgtgagaaaa gatgagaatt ttcaaataca tccattcata tatcgggatg
     8221 ttgtgcgtgt tctgaaatct cttaacatgc tgcattagga gggtgtaatg aaatctatat
     8281 ttacggtatt atcattcatt attgctataa acgcaggaaa cagcgaaaac tgtcaatcta
     8341 tagacaaaag gtatgtcgaa gaagactttt ttaatattga caaggtcagt tttgctgaag
     8401 aattagaaaa accatcggaa acacaatcag aaaaactgcc ggaatctgat gatgctgaaa
     8461 acaaaaatcc tgtttcggga acaataatag actcaaagcc ccagatagaa tggaaagttt
     8521 ctttcggtaa agtgttctgg atgatcatca tctttctttt tgcctatatg ctcataaaat
     8581 attttacaaa agcacttgag gcaattgctg aaaaatgggc ggcttcccgt cttttcataa
     8641 aaagagtgat tcctgtattc cggctttctg gctggattgc gacaatatat ctgatgatag
     8701 tcggagttct tgctcctcca attgaaacaa tacttgcagt tactgcgtca ttcggtattg
     8761 cgatgggttt tgcgtctcag gatatactta aaaatatatt cggcggaata atgatccttt
     8821 ttgacaatcc tttccaggtc ggtgataaga tacaggtcgg ggaacattac ggagaggtga
     8881 tccacatcgg cttaagatcg gtaagaattg tcacccctga tgacagcacg gtttcaattc
     8941 ccaatagtga tattgttagc agatcggttt ccaattcaaa tacaggcgag ttcaactgtc
     9001 aggttgttgc cgagttctat ttcccaattg atgttgatct tgaaaaactg agaaaacttg
     9061 cgacaagagc ggcaattgtc tcaaaatatg tttatttgag aaaaccagtt gcagtaagat
     9121 ttaagaatga aattcatgaa ggccgttcaa tggtgaaaat gaggttgaag gcatatgttc
     9181 ttgatttgag atacgagttc gctttttcaa cagaaatgag cgaaatagtg atatcggaag
     9241 ttttaaaaca gggacttgtc acccctcagc agatgtcagg atagtttaag atctatagag
     9301 ggcatggagt gaagtacgga tcatttctga aagatgtttt tatctgttca cttggagctt
     9361 acggtggtcc ggaagcccat ttcagcatat ttcttgacca aatggttgtc aaaaagaagt
     9421 atctgaccga agaggagctt gttgaacttg ttgcgctctg cagtattctg ccggggccga
     9481 cgagtactca ggcacttgtt tcgatcgggt ataaaaacgg cgggccgctt ctaggttttc
     9541 tgacaatgat tgtctgggca ctgccggttc tgatggtgat gacggcattg tcgtttcttt
     9601 atcagtttct ttcttttcag gggatttcaa gcggtgtttt acggtttatc ggtccgatgg
     9661 ctgtaggttt cataattgtc gccgcattca ggatcggcag aaaggttgta aaagacattc
     9721 cgaccggagc gttgatgctt tttggagctg ttacgacatt ttttatccgt gctccgtgga
     9781 tctttccggt tgtgctgctg atcggcggag cggtcacgat ctttctttca agggaaaaag
     9841 ggatctggaa taaaatcaag cttgctcctc catggggcta tctatttgtt tttgctgtga
     9901 ttgcagttct tgcaatagtt cttgcgacag ttactgacat aaggatgttt cagctttttg
     9961 aaagtttcta ccgttacgga tatctcgttt tcggcggagg gcaggtgatc gttcctgtca
    10021 tgcacagtga acttgtcgaa gtgaggcagt acatcacaaa tgaagagttt ctgaccggtt
    10081 acgggcttgt tcagggactt ccgggaccga tgttcagctt tactgcctat gccggtggaa
    10141 tggcagcccg tgacagcgga atttttgttc agataatgag tgcggcggta agcggaatcg
    10201 gcattttcct tccaggagtg cttctcatct attttgtcta tccagtctgg gaggaactta
    10261 aacagatcaa agcggtcaaa atatcactca ccggtatcaa tgccgttgca ggcggactca
    10321 ttgcagttgc cgcagtgata ttaatgaaag caagcggatt tcagatcgaa aacctcattg
    10381 tcacagcagt ttcagtcgca cttctgatgt caagaaaagt tccagcaccc ctgatcgttg
    10441 tcgccgcaat ccttggcgga gtgtttgtct gaaattgctt aactccctaa attaaaacaa
    10501 cttttttcct aatttccatt gactaaataa tttgcatcaa atttcttgca aatttaatct
    10561 gattactgca ttatctattg gtgactaaac tttcggagtc ggaaaatgac agaaaatgag
    10621 ttgataaata ttgatgatat taaaagcagg attttcacaa ttcgcggaat gcaggtaatg
    10681 ctggacagtg acttggctga atttttcgga gttgagacaa agcgacttaa tgaacaggtg
    10741 cggaggaata ttttaagatt tcctgaagaa ttctgtttta aattaagtaa agaagaaaag
    10801 aataaggtgg tcgcaatttg cgaccacctt caaaatctga agtattctca tcatcttcca
    10861 tctgttttta ctgaacaggg tgttgccatg gcatctgctt taattaacag cgacattgct
    10921 gtgatgatga gcataaaaat tatgaaagca tttgtagcga tgcggcgttt tatttcaaac
    10981 aatggtgatg tatttaaaag aatggaaact gttgaaataa aactgcttga aacggacagc
    11041 aaaatcgaaa aagttctttc tgctcttgaa tcaaagcaaa ttcagccaaa acaaggcatc
    11101 ttttttgaag gtcaggtttt tgaagcacac aaattcgttt cagatattgt gagatcggct
    11161 gaaaaatcca ttattct
//
LOCUS       DAOOYB010000083        11127 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_6259_length_11127_cov_9.465047, whole genome shotgun sequence.
ACCESSION   DAOOYB010000083 DAOOYB010000000
VERSION     DAOOYB010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11127
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6259_length_11127_cov_9.465047"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="PL056_06430"
     CDS             complement(<1..186)
                     /locus_tag="PL056_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46685.1"
                     /translation="MSRIILVSLVLFSFLNLFPQDTVPEEAAVEPSEESSGDSAQAEE
                     PAPAEEPAPAEEVSPAEE"
     gene            complement(201..626)
                     /locus_tag="PL056_06435"
     CDS             complement(201..626)
                     /locus_tag="PL056_06435"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46686.1"
                     /translation="MRVEQISVLLRNEPGVLSKILESISNGNINILGLTINETAAFGE
                     LRLVVSDTQKTKGILEKIDVSYNVVKIIVISLPDKPGELMKIAKLLSDNDINIDYMYT
                     LSAVRNSAFIAIKSWDMPATEEILGEKGFKIVSINEITK"
     gene            complement(709..1128)
                     /locus_tag="PL056_06440"
     CDS             complement(709..1128)
                     /locus_tag="PL056_06440"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HPM46687.1"
                     /translation="MKKYYSYLLIAFLLSGVVLLNLSLSRDDNAGINGAGDFPDGVKV
                     ISFSSPHCGACEGVKKEVEKVRSELEGDAVFESIDVTEDIQTADYYRIAVVPTILITY
                     QGIEVDRFTGSKTAKELSAAIKKEIGFPKYCEDGTTC"
     gene            1277..2620
                     /locus_tag="PL056_06445"
     CDS             1277..2620
                     /locus_tag="PL056_06445"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HPM46688.1"
                     /translation="MKKCMLIETIPCGVFVTDRDYNVHFVNETMLELAGVTKNLVSRR
                     GIAAFEFRRSGGELMKPDDSDCLCKKGEKVIQRHLYLSKKAGGLRPVFISGISSSFEK
                     ISDAIIFCVTDLTRLEGCNILPALERSKTQFHNMIGESEPMKMLFRHIELASASDVTV
                     LITGESGTGKELVASAIHNESSRRKAAFIKVNCSSLAENLLESELFGHARGSFTGAIS
                     DRPGKFEAADKGTLFLDEIGDISPSTQVKLLRAVQEKVVTRVGENRERRVDIRLITAT
                     NRDLRQMVEQGKFREDLFFRLNVFPLRTAPLRERGSDIFLITEHLIEKLNLKYGKSVS
                     GMSRSALQIIMDHPWPGNIRELENALEYAFIFRSDGEITSADLPESIISSKTVYTAET
                     IGNGPKQNKFSRAPVNAEELAQLLKKYKSARKDVAAHLGISTVALWKKMKKFNLL"
     gene            2630..3256
                     /locus_tag="PL056_06450"
     CDS             2630..3256
                     /locus_tag="PL056_06450"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPM46689.1"
                     /translation="MDTRTAQYLSDFLKHDAEYLDGLRKSNRSRNDIQPMIEMEIGKL
                     LIFLIKLTKAKKVLELGTSNGYSALWMAHALKGTGGHITTIEGHDRMAEEAQKNLNEA
                     GVDNVELIKGKIETVLPELIAKKKKYDLIFQDSGKYLYPLMLENTVKLCRKGGIIIAD
                     DTLFRVIETVRSGLGDYTHDYNRAVFAHKKLFSTILPIGHGLTMSYRV"
     gene            3351..5255
                     /locus_tag="PL056_06455"
     CDS             3351..5255
                     /locus_tag="PL056_06455"
                     /inference="COORDINATES: protein motif:HMM:NF036933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgGFc-binding protein"
                     /protein_id="HPM46690.1"
                     /translation="MKKTFIFIIFAITVFFLSCTKDEESVSENENINDSEATDKDPSA
                     DGDSAGSDTENSDDNSSKTDDDSKETPDNENPSITCTPGMRVCNPSDNDAVYECNAEG
                     TGLNETPVETCEEGLVCFFGKCESAACASSGSSYEGCEFYTAKLYNGRKTELVTEHDS
                     FSVAIANTHPTLTATIKLLKTLPDGTETPVSEFAYCYKTVENDIFGNPYDAMKCDNKD
                     SGFAITIPPKEIILVTPRKDDRMLIGTSASFLSYHIQSDVPVTIYQFNPFENLSSNDA
                     SLLFPSKKLGTDYYVSTYEDQTGVGSDGPGYFTVIGVNNMDVELEIVPTVEIKGGGGI
                     PASPAGTPFNVTVRKGEVLNITSGNNLDDLTGTRIYCKNPLNNCAPFAVFGGHGCANI
                     PKDRGYCDHLEHQLLPVNRWGQRYAVVKTRPREKERDVIRIVASEDSTNVKIKTMDDK
                     QESGNEFNVTLNKGQWHQYEIYYFWGDGFDPGSSFIEADKPVQVVQYLSGSEDQSFDC
                     RDSTTGSSHTGCYGDPAMSIIPPVEQFRNEYFFFSSSVKGEDDGNENFVQVVTDQGVF
                     VTLDETTPVPVKQGTVYGSDKIFYIFELDEDFMRHQLNCTGPCGILIYGWGMDVSYMY
                     PGGLNLRVLK"
     gene            5368..5559
                     /locus_tag="PL056_06460"
     CDS             5368..5559
                     /locus_tag="PL056_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46691.1"
                     /translation="MVSMTERLRETGREEGRLEEKNTVCKKAILAGISNELIVTITAL
                     PLETVVAMQKELRKKKPVY"
     gene            complement(5569..5898)
                     /locus_tag="PL056_06465"
     CDS             complement(5569..5898)
                     /locus_tag="PL056_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000135495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HPM46692.1"
                     /translation="MSTSKEFIDYILENIEDAGVVTVRKMFGEYAVYCDGKVIALVCD
                     NQFFLKPTKAGRDFIGDVIEAPPYNGAKPHYLIEDGIDDCKWFSRLIKITANEVPEPK
                     PKKKKAK"
     gene            6113..6910
                     /locus_tag="PL056_06470"
     CDS             6113..6910
                     /locus_tag="PL056_06470"
                     /inference="COORDINATES: protein motif:HMM:NF014460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPM46693.1"
                     /translation="MRSAIENIGNRTIAKYNDIANFSSFVLRCLKAIGSGSFFNRAVF
                     DVLVIQIFFTAYELLSLFIFLSVVYGSIFIGIVLQTVKSLGLVQYLGDIIMGLVVLEM
                     APLITVFLLALRSSSAVNAEIAVMKVNKEIDALESFGIDPVKYLFVPRIISFVLSVTI
                     LSSVFSIVVLFSGFIFSSIIFQMSPGMYADLIAKSVTATDLLVMLLKSMTFGFLISVI
                     PLYHGWRTKYMMTAIPVAVLKGMLSVFMSIILVEVLSLAARLALNYL"
     gene            6907..7350
                     /locus_tag="PL056_06475"
     CDS             6907..7350
                     /locus_tag="PL056_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46694.1"
                     /translation="MNEPDFDSWFAEHINLAKTAFVSPDIPLLSNLTAIDNIALVARF
                     HSDIPDKMIFSNIKALMELLDINSSINSKPSSFDEMTRFKVKLLRAIMMTGADIVIDR
                     PFYMLRNMPTVQSIRKILTLLDTHFEQCIIADYEWHRDRYGDLIE"
     gene            7343..8284
                     /locus_tag="PL056_06480"
     CDS             7343..8284
                     /locus_tag="PL056_06480"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HPM46695.1"
                     /translation="MSRSAGFKVGIFLIISVVAIIVFVIFLLIQKDFFTDHRFYTLSS
                     PSGEGITEGMPLLFSGFEIGKVATLELDPAGNVVISVKVPQRHAERINKSSTFTLEKP
                     FIGSPRFVVKTPDINAPEVDEELVFPTDTIDDINEVIKKLQPMVEKVDEIATNVKVMT
                     GKESDLTKTLANAETITNELARKQGVIEMLAGDPKTAKDFKESISSLNTALKEAELLI
                     SNANKTLLKADEQILGEEGTVKKVNSIIDDIEKKMKELDSLVSNLVKTGENVENATND
                     LEMLRKDVDMTLNSVNIMIQDVRNALPIEQKKEIKLP"
     gene            8281..8934
                     /locus_tag="PL056_06485"
     CDS             8281..8934
                     /locus_tag="PL056_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46696.1"
                     /translation="MKKIVPLYFLSILILSCSSQPAPAWQQESFVNMNNFISNFMEGN
                     DKFSESYYKKLVENLEMTTDPDEIVKAHLIKCSLDYSLMNYRTCTEAEPLLDILRKNE
                     NTVYYNFISSKNSDIPEKYKDIEKHTSPCDTAKLNKAVKKLDEPLSKLIASSFAVKKD
                     CYDENTLLNAVETASKEGWKKAALSYLTFTAEHFDRKGESEKAENLRKRIDSLNKKD"
     gene            8957..9451
                     /locus_tag="PL056_06490"
     CDS             8957..9451
                     /locus_tag="PL056_06490"
                     /inference="COORDINATES: protein motif:HMM:NF025226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HPM46697.1"
                     /translation="MKRSNWLVFLIIFIVSSCGSVETRSQGTDEQKSGTSKKQEGAVK
                     GSSVNSGDIEQLVEAIRTGNKDAAVKMIDGGIDVNSKNKSGVYPLHWAVAKGDNDLVK
                     ILLGKKAYINATDGYREEPVIMWAAKKGHFETVKMLIEKGAAVNIRQIQRDASYGCSP
                     LRSQ"
     gene            9381..10085
                     /locus_tag="PL056_06495"
     CDS             9381..10085
                     /locus_tag="PL056_06495"
                     /inference="COORDINATES: protein motif:HMM:NF025226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HPM46698.1"
                     /translation="MPQSISDRYKETPLMDAARYGHNEMLKLFLNNRANINDVTVDGM
                     SALMFASQNGHTEIVKTLLEYRADINKIHFKTSKTALGYAKERNNKDIIAMISKQAKV
                     EGDLIKRPAKPEDTEFVTTPPDEATLRKAWAGREDYLPASLNGKQVMVHMNKFLPDIT
                     QCYQNRYEQGDRNMMGTMELMVRVAGSGKILDIYFTTEKYQSSLFGDCILDAIKSRDF
                     PMFYDGQVDFKYSYSI"
     gene            complement(10082..10852)
                     /gene="rhuM"
                     /locus_tag="PL056_06500"
     CDS             complement(10082..10852)
                     /gene="rhuM"
                     /locus_tag="PL056_06500"
                     /inference="COORDINATES: protein motif:HMM:NF024706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
                     /protein_id="HPM46699.1"
                     /translation="MQNQMAELFQTDRSSIAKHIGNVYKSLELDRLSTCAKIAQVQIE
                     GDRKVSRKIEYYNLDMIISVGYRVNSLRGTQFRIWANKVLKDYLMKGYALNQRIEQIE
                     EKLFEHDKKFELIIQQAIPLKEGIFFDGQVFDAYELVVSFIKEAKKSIVLIDNYVDET
                     VLSMLSKRKDQVKATIYTANLSKELSLDLKKHNAQYEPVEIKVFSKSHDRFLIIDEKI
                     VYHIGGSLKDLGKKLFAFSRIEIDPQIILNYLVEFRQL"
BASE COUNT         3502 a         2287 c         2266 g         3072 t
ORIGIN      
        1 ttcttcagca gggctcacct cttcagcagg cgccggttct tcagcaggtg cgggttcttc
       61 agcttgcgca gaatccccgg aagactcctc agatggctca acagccgcct cttcgggaac
      121 tgtatcctga ggaaaaagat ttaaaaagga aaacaggact aaagaaacaa gaattattct
      181 tgacatttat ccccctggat ttatttggtt atttcgttaa ttgaaactat cttgaaccct
      241 ttttcgccaa gtatttcttc tgtagcaggc atatcccagc tctttatagc tatgaatgca
      301 ctgttcctta ctgcagaaag agtgtacata taatcaatat tgatgtcgtt atcactcaaa
      361 agttttgcga tcttcatcaa ttctcccggt ttgtcaggaa gcgaaattac aattatttta
      421 actacattgt atgaaacatc tatcttttcc agaatgccct ttgtcttctg tgtgtcgctg
      481 acaaccagac gaagctctcc gaaagcagcg gtttcgttga tggtaagacc aagaatattt
      541 atgttgccgt tacttattga ttcaagtatt ttgctcaaaa ctcccggttc atttctcaaa
      601 aggactgaaa tctgttcaac tctcatttac ccctccgaat aaatttatgc cggtcggtaa
      661 catagcactt aaaagtatgt attgtcaaaa ttttagaaaa aattgtgatc agcaggttgt
      721 tccgtcttcg caatacttag gaaaacctat ctcttttttt atagctgcag agagttcttt
      781 cgcagttttc gatcctgtaa atctgtcaac ttctattccc tgataagtta tcagaattgt
      841 tggaactact gcgattctat agtagtctgc ggtctgtatg tcctctgtaa catcgatcga
      901 ttcaaaaacg gcgtctcctt cgagttcact gcgaactttt tcaacttcct ttttaacccc
      961 ttcgcaagct ccgcaatgag gagaagaaaa agaaataacc ttaactccgt caggaaagtc
     1021 accggctcca tttatcccgg cattatcatc tctgctcaga gacagattaa gcaaaaccac
     1081 gccggacagc aaaaaagcga ttaacagata agaatagtac ttcttcataa ttcctccaca
     1141 aaaaaaagac aacaactgca tacaataacc gtgcctgaaa aaccggttga aaaacgaact
     1201 ttaagaaata acttgcatta acttatctaa ttatgttata ttaacttgtt tttgttaatt
     1261 aactttaagg tgccacatga aaaaatgcat gctgatagaa acaatacctt gcggggtgtt
     1321 cgtaaccgac agagattata atgtccactt cgtaaatgaa acaatgcttg aacttgcggg
     1381 agttacgaaa aatcttgttt caagacgggg tatcgccgct tttgaattca gaagatcggg
     1441 aggggaactt atgaaaccgg atgacagcga ctgtctgtgc aaaaagggtg agaaggtcat
     1501 ccagcggcat ctttacctgt caaaaaaagc cggcgggctg aggcctgttt tcatttccgg
     1561 cataagctca tcttttgaaa agatctctga tgccattatt ttctgtgtaa ctgatctcac
     1621 acggcttgaa ggatgcaata ttcttcctgc ccttgagaga tcaaaaacac agtttcacaa
     1681 catgatcggt gaaagtgagc cgatgaaaat gcttttcagg catattgagc ttgcttcagc
     1741 gagcgatgta acagtactca taacaggtga aagcgggacg ggaaaagaac ttgtggcttc
     1801 agcgatacac aacgaaagtt caaggagaaa agccgctttt attaaagtca actgctcgtc
     1861 tttggctgag aaccttcttg agagcgaact tttcggtcac gccaggggtt ctttcaccgg
     1921 tgcgataagc gatagacccg gaaaattcga agcggccgac aaggggaccc tttttcttga
     1981 tgaaataggt gatatatctc cttcaacgca ggtcaaactg cttcgggccg ttcaggaaaa
     2041 agttgtgaca agggtcggag agaacaggga aaggcgggtt gacatccggc tcatcactgc
     2101 aactaacaga gatctaagac agatggttga gcagggaaaa ttcagggagg acctcttttt
     2161 ccggctcaat gttttccctt tgagaacggc acctctgagg gaaaggggtt cagacatctt
     2221 tctgataacg gagcatctga tcgaaaaact gaacttaaaa tatggaaaaa gtgtttcagg
     2281 tatgagcagg tcagctctgc agataattat ggaccatcca tggcccggca atatcagaga
     2341 gctcgaaaac gcactggaat atgccttcat tttcagaagt gacggtgaga taacatcagc
     2401 ggaccttccc gaaagcatca tttcatcaaa gaccgtgtat actgcagaaa ccattggaaa
     2461 cggcccgaaa cagaataaat tcagcagagc tccggtaaac gctgaagaac ttgcgcaact
     2521 gcttaaaaag tataaatctg caaggaagga tgttgcggca catcttggga taagcactgt
     2581 cgctttgtgg aaaaaaatga aaaaattcaa cttgttataa ggagtcaaca tggacacaag
     2641 aacagcacag tatctgagcg attttttaaa gcacgatgcc gaatatcttg acgggttaag
     2701 aaaatcaaac aggagtcgta atgatatcca gcccatgatc gaaatggaaa tcggaaaact
     2761 gctgattttt cttataaaac ttacaaaagc caaaaaagtg cttgaacttg gcacaagcaa
     2821 cggttattca gcgctctgga tggcacacgc tctgaaagga acaggcggac atatcaccac
     2881 cattgaaggt cacgacagaa tggctgagga agcgcagaaa aacctgaatg aagcaggagt
     2941 tgacaatgtt gagcttataa aaggaaagat cgaaactgtt cttcctgaac ttattgcaaa
     3001 aaagaaaaag tatgacctga tctttcagga cagcggaaaa tatctttatc cgttaatgct
     3061 tgaaaatact gtgaaattat gcagaaaagg ggggatcatc atcgctgacg acaccctttt
     3121 cagggtcatt gaaacagtaa gaagcggact tggcgactac acccacgact ataacagagc
     3181 cgtttttgcc cacaaaaaac tcttttccac aatacttccc atcggacatg gactgacaat
     3241 gagttaccgt gtgtgaaccg aattcctgtt tttcatcatc tgtttttttg acataaagtt
     3301 gcatatcacc tattatcggc ctgtgtgtga attaattttg gagattttcc atgaaaaaga
     3361 cttttatttt cattattttt gcaattacgg tgttcttcct ttcctgtaca aaagatgaag
     3421 agtccgtttc cgaaaatgag aatatcaatg actctgaagc gaccgacaag gatccgtctg
     3481 ctgacggtga ttcagccggc agtgacaccg aaaactcaga tgacaactca tctaaaaccg
     3541 atgatgacag caaagagacc cctgacaatg aaaatccgtc aataacctgc actcccggaa
     3601 tgagggtctg taacccttct gacaatgacg cagtctatga atgcaatgct gaaggaacag
     3661 gtctgaatga aactcctgta gaaacatgtg aagagggact cgtctgtttt ttcggaaaat
     3721 gtgaatctgc tgcgtgtgca tcatcaggaa gcagttacga aggttgcgaa ttctacacag
     3781 caaaactcta caacggcaga aaaacagaac ttgtgacaga gcatgacagt ttttcagtcg
     3841 cgatcgcgaa cacacacccg actcttactg caacaataaa actgcttaaa acccttcctg
     3901 acggaacaga aacaccggtc agtgaattcg cctactgtta taagactgtt gaaaatgaca
     3961 tcttcggcaa tccttatgat gcgatgaaat gtgacaacaa agattccggc tttgccatca
     4021 ccattccgcc aaaagagatc atccttgtaa ctccgagaaa agacgacaga atgcttatcg
     4081 ggacttcggc aagtttcctg agttatcaca ttcagagcga tgttcctgta acgatctatc
     4141 agttcaaccc ttttgaaaat ctttcatcaa atgacgcatc actgctgttc ccttcgaaga
     4201 aacttggaac tgattactat gtttcaactt atgaagacca gactggagtg ggaagcgacg
     4261 gtccgggata tttcaccgtg ataggggtaa ataatatgga tgtcgagctg gaaatagttc
     4321 cgacagtcga aataaaggga ggcggcggaa tccctgcatc cccggcaggc acacctttca
     4381 atgtcacagt cagaaaagga gaagtgctta atattacaag cggaaataat ctggatgacc
     4441 ttacagggac aagaatttac tgtaaaaatc cgcttaataa ctgcgcacct tttgcagttt
     4501 tcggaggaca tggatgtgca aacattccca aagacagagg atactgcgat catcttgaac
     4561 atcagcttct gcctgtgaac cgctggggtc agcgttatgc tgttgtaaaa accaggccga
     4621 gagagaaaga aagggatgta ataagaattg tggcatcgga agattctaca aatgtcaaaa
     4681 taaagaccat ggacgacaaa caggaatcgg gaaacgaatt taatgtcact ttaaataaag
     4741 gccagtggca ccagtatgag atctattatt tctggggtga cggttttgac cccggctcat
     4801 cgttcattga agcggataag ccggttcagg ttgtccaata tctcagcggg tcggaagatc
     4861 agagctttga ctgccgcgac agcactaccg gctcttcaca tactggctgt tacggagatc
     4921 cggcaatgtc aataatccct ccagttgaac agttcagaaa tgaatatttc tttttttcaa
     4981 gctctgttaa aggggaagat gacggaaatg agaattttgt ccaggtcgtt accgatcagg
     5041 gcgtttttgt cacacttgat gaaaccactc ccgttccggt aaaacaggga acagtttacg
     5101 gctctgataa aatattctac atttttgaac ttgatgaaga ttttatgcga catcagctca
     5161 actgcaccgg accttgcgga atactgatct atggatgggg aatggatgta agttatatgt
     5221 atccgggcgg actcaatctg agagttctga aatagtatct tgacttaaca tcaccaaaac
     5281 catatcatta cttaatgaaa taagattttg tcccgcaccc ctatattttc attaaagttg
     5341 taatttaaat cagtacgagg cagtaaaatg gtatcaatga cagaaagact cagagaaaca
     5401 ggccgtgaag aaggcagact tgaagaaaag aacacagtat gcaaaaaagc gatcctcgcc
     5461 ggaatttcaa acgaacttat cgtgacaatt actgctcttc cccttgaaac agtggttgca
     5521 atgcagaaag aactcagaaa aaagaaaccg gtctattaag aatcatcatc acttagcttt
     5581 cttctttttg ggcttaggct cgggaacttc atttgccgtt atctttatca acctgctgaa
     5641 ccatttgcaa tcatcaatac catcctcgat aagataatgc ggctttgcac cattgtaagg
     5701 cggcgcctca atgacatcac cgatgaaatc acgccctgcc ttagtcggtt tcaggaaaaa
     5761 ctgattgtca caaacaagag ctatcacctt cccgtcacag taaaccgcat attcgccgaa
     5821 catcttcctg acagtgacaa ctcccgcatc ttcaatgttt tcaaggatat agtctataaa
     5881 ttcttttgaa gtggacatta actactccag atattttcta taatcacata aaaaaatgac
     5941 ccgctttcag atcttacggt atgcagaacg ggtactgttg gaaaaaacta acttattttt
     6001 tatttttatc aactttttta aatattcacc aatggttttg acagattgat tctctaaatt
     6061 gtaatatttt ttgcgttgtg tataatcaat ttgcattttt ttgaggttta ttttgagatc
     6121 ggcgatagag aacatcggca acagaaccat tgcaaaatac aatgatatag cgaatttttc
     6181 ttcatttgtc ctcagatgtc tcaaggcgat cgggtcaggg tcgtttttca accgtgctgt
     6241 attcgatgta cttgtaatcc agatattctt cacggcttat gaacttctgt ctcttttcat
     6301 atttttaagt gtcgtttacg ggagcatttt catcggaata gttctgcaga cggtcaaaag
     6361 cttggggctt gtccagtatc ttggcgacat catcatgggg ctcgtggtgc ttgaaatggc
     6421 accgctcata acagtttttc tgctcgctct tagaagttca agcgccgtca atgccgaaat
     6481 tgcagtgatg aaagtcaaca aagagataga cgcccttgaa tcgttcggaa tagatccggt
     6541 gaaatatctt tttgtgccga gaataatcag ctttgtcctg agtgtcacga tcctttcttc
     6601 cgttttttcg atcgtcgtcc ttttcagcgg attcatcttt tcaagcatca tttttcagat
     6661 gagtcccgga atgtatgcgg atctcattgc aaaatctgta acggcgacag atcttcttgt
     6721 aatgctgtta aaaagcatga ctttcggatt cttaatatca gtcattcccc tttatcacgg
     6781 ctggcggaca aaatatatga tgaccgccat ccctgttgcc gttttaaaag gaatgctgag
     6841 cgttttcatg tcaattatac ttgtggaggt gctgtcatta gcggcaaggt tagcgttaaa
     6901 ttatttgtga acgaacccga ttttgattcg tggtttgccg aacacatcaa cctggctaaa
     6961 actgcattcg tctctcctga cattcctctc ctttcaaatc tcacagctat tgacaacatc
     7021 gcacttgttg caaggttcca ttcagacatt ccagacaaaa tgatattcag caacataaaa
     7081 gctctcatgg aacttcttga cataaattcc tcaataaatt caaaaccttc ctcgtttgat
     7141 gaaatgacaa ggttcaaggt gaaactttta agggctatca tgatgacggg ggcagacatc
     7201 gtcattgaca gaccttttta tatgctgaga aatatgccaa cagttcagtc gataagaaaa
     7261 atacttacat tgcttgacac acattttgag cagtgtatca ttgcagatta tgagtggcac
     7321 cgtgacagat acggagattt gattgagtag atcggctggc ttcaaagtcg gaatttttct
     7381 tattatttca gtcgttgcca taattgtgtt cgtaatattt ctccttattc agaaagattt
     7441 tttcacagat caccgctttt ataccctttc atcaccgagc ggagaaggga taacagaagg
     7501 aatgcctctg ttgttttccg gctttgagat aggaaaagtt gccacacttg aacttgatcc
     7561 tgcaggcaat gttgtcatct cagtaaaagt ccctcaaagg catgccgaac ggataaacaa
     7621 aagctcaact ttcactcttg aaaaaccgtt catcggctct ccgagatttg tggtgaaaac
     7681 tcccgatatc aatgctcccg aagttgatga agaactcgtt ttcccgactg acaccattga
     7741 tgatatcaac gaagtcataa aaaagcttca gccgatggtt gaaaaagtgg atgagatcgc
     7801 aacaaatgtg aaagtgatga caggcaaaga gagcgatctt acaaaaacac ttgcaaacgc
     7861 cgaaactatt acaaatgaac tcgcaaggaa acagggtgta atcgaaatgc ttgcaggtga
     7921 tccaaagaca gcaaaagact tcaaggaaag catctcatcc cttaacacag ctctgaaaga
     7981 agcggaactg cttatatcaa atgccaacaa aacccttttg aaagctgatg aacagatact
     8041 tggagaagaa ggaactgtta aaaaagtgaa cagtataatt gatgacatcg agaaaaaaat
     8101 gaaagagctt gattctcttg tttcaaacct tgtcaaaaca ggtgaaaatg ttgaaaatgc
     8161 gaccaatgac cttgagatgc tccgtaaaga tgtcgatatg actctgaact ctgtaaacat
     8221 aatgatccag gatgtcagga acgcccttcc gatcgaacag aagaaggaga taaaactgcc
     8281 atgaaaaaga tcgttcccct ttatttttta tcaattctta tcctttcatg cagttctcag
     8341 cccgcacctg catggcagca ggaaagtttt gtgaacatga acaatttcat atcaaatttc
     8401 atggaaggga acgataaatt ttcagaaagt tactataaaa aactggttga aaatcttgaa
     8461 atgacaacag atcccgatga aatagtaaaa gcacatctta ttaaatgctc ccttgactat
     8521 tccttaatga actacagaac ctgcacagaa gcggaaccat tgctggacat tctcagaaag
     8581 aatgaaaata ccgtttatta caatttcatt tcttcaaaaa attcagatat tcctgaaaaa
     8641 tataaggaca tcgaaaaaca cacttcaccc tgcgatactg caaaactgaa caaagcggta
     8701 aaaaaacttg atgaaccgct ttcaaaactg atcgcctctt cttttgccgt aaaaaaggac
     8761 tgttatgatg aaaacactct gctcaatgct gttgaaacgg catcaaagga agggtggaaa
     8821 aaagcggctt taagttattt gacattcact gcggaacatt ttgacagaaa gggtgaatct
     8881 gaaaaagctg aaaatctcag gaaaagaatt gactccttaa ataaaaaaga ttgaccggat
     8941 tttaacggag gttaatatga aaagatctaa ctggctggtt ttcttaatta ttttcattgt
     9001 cagctcatgc ggttccgttg aaacaagatc gcagggcaca gatgaacaaa agtccggcac
     9061 atcaaaaaaa caggaaggtg ctgtcaaagg atcatccgtt aattccggcg acattgaaca
     9121 gctcgttgaa gcaataagaa cgggaaataa ggatgccgca gtaaaaatga tcgacggcgg
     9181 aatcgatgtg aattcaaaga acaaaagcgg tgtctatccc cttcactggg ctgtggctaa
     9241 gggagacaac gaccttgtca aaatacttct tgggaaaaaa gcatacatca atgcaactga
     9301 cggataccgt gaagagccgg taataatgtg ggcggcaaaa aaagggcatt ttgaaactgt
     9361 gaaaatgctc attgaaaaag gtgccgcagt caatatcaga cagatacaaa gagacgcctc
     9421 ttatggatgc agcccgttac ggtcacaatg aaatgctcaa gctctttctc aataacaggg
     9481 cgaatatcaa cgatgtgaca gttgacggaa tgagcgcttt aatgttcgct tcacagaacg
     9541 gtcatacaga aatagtcaaa acccttcttg aataccgtgc cgacataaac aagatacact
     9601 ttaaaacaag taagacggca ctcggctatg caaaagaaag gaacaacaaa gatataattg
     9661 caatgatctc aaaacaggcg aaagttgaag gagatctcat aaaaagaccg gcaaagccgg
     9721 aagacactga attcgttacg actcctcccg atgaagcgac actcaggaaa gcgtgggcag
     9781 ggagagaaga ttatcttcct gcatcactta atggaaaaca ggtcatggtc cacatgaaca
     9841 aatttctccc tgacatcacc cagtgctacc agaacagata cgaacagggc gacaggaata
     9901 tgatgggaac aatggaacta atggtaagag ttgcaggaag cggaaaaata ctcgatatct
     9961 atttcacaac agagaaatat cagtcatccc ttttcggtga ctgcatactt gatgcaatta
    10021 aatcacggga ttttcccatg ttctatgacg gacaggtcga tttcaaatat agttattcaa
    10081 tttaaagctg cctaaattcc accaaataat tcaagataat ttgagggtca atttctattc
    10141 ttgaaaatgc gaacagcttt ttcccgagat ctttgagtga accgccgata tgatagacaa
    10201 ttttttcatc aattatcaga aatcggtcat gtgacttgct gaaaaccttt atttcaacag
    10261 gttcgtattg agcattgtgt tttttaagat caagagaaag ttcttttgaa agattggcag
    10321 tataaatagt tgctttaact tgatctttcc gtttcgaaag cattgataac actgtttcat
    10381 ccacataatt atcaatcaga acaatagatt ttttcgcttc ttttatgaat gaaacgacaa
    10441 gttcataagc atcgaacacc tgcccgtcaa agaatattcc ttctttcagt gggattgcct
    10501 gctgaattat cagttcaaac tttttatcgt gttcaaacag tttttcttcg atctgctcga
    10561 tccgctgatt gagtgcatac cctttcataa ggtagtcttt cagcacttta tttgcccaga
    10621 ttcgaaactg agtgcctctt aaagaattaa cacggtatcc gacagaaatg atcatatcga
    10681 gattgtaata ttcaattttt cttgaaactt tccgatctcc ttcaatttga acctgtgcaa
    10741 tttttgcaca ggttgataat ctgtctaatt ccagcgactt atatacattg ccaatatgct
    10801 ttgcgattga tgaccggtct gtttgaaaaa gctccgccat ctgattttgc atcttttcct
    10861 ctaattaaaa ctaaacacta tcaaatatag cgactcgaat cattttcgtc aaattattta
    10921 tattactcct tgattaattt atgattttga gataatctga tccgttaatt atttgatttt
    10981 ttaaggtttt atgaagcacg cgattctgat tttcacactt tttctgtttt cctgcacatc
    11041 ctgcaacggc aataacccga ggaacgacac tgaaaacgac attgatatga ttcaacctga
    11101 caaagattcc gacgacttgt ccgacga
//
LOCUS       DAOOYB010000084        11070 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_6313_length_11070_cov_11.064911, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000084 DAOOYB010000000
VERSION     DAOOYB010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11070
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6313_length_11070_cov_11.064911"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..537)
                     /locus_tag="PL056_06505"
     CDS             complement(31..537)
                     /locus_tag="PL056_06505"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HPM46700.1"
                     /translation="MEKRIFLMIAVVFLSFQMSGDEDVKRFDLYTLDDVLYSSQKELE
                     KNDTKIMVADFFSIHCEPCKKSISKWDEVYRKYSAKGLKFVVIVIPVEEDRSAELVKI
                     KEFFKKQKISFPVVFDKYSITGKKFGIVDNNGSAVVPQMFMLDKTGRIIFKSEKHPEL
                     IKEIEKSL"
     gene            complement(652..948)
                     /locus_tag="PL056_06510"
     CDS             complement(652..948)
                     /locus_tag="PL056_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46701.1"
                     /translation="MHYFAFLSYEVLKAENYNHDFFNGELEEIGLYSKIKTEGDTLRD
                     LPRYTYIGEYKFADKEELKNVLYSEVKKLFQSNGVQVTLFIGVSEKATIGLDIL"
     gene            complement(957..1502)
                     /locus_tag="PL056_06515"
     CDS             complement(957..1502)
                     /locus_tag="PL056_06515"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HPM46702.1"
                     /translation="MKIKSKKILMIIAPEKFRDEEFEIPFKKFVDEGASVTVASLKKG
                     VATGMFGAKFDVKKTLDEVNESDFDAVVFVGGAGVPSVRADKRAVEIAKNSAGHEVLG
                     AICWAPTILAKAGAVKGKKTTVWLGDDAEYGMKTSAVMEKYGAVFSGGEVIEDGNIIT
                     GNGPAAAEKFAIAIVNKLSSK"
     gene            complement(1492..2679)
                     /locus_tag="PL056_06520"
     CDS             complement(1492..2679)
                     /locus_tag="PL056_06520"
                     /inference="COORDINATES: protein motif:HMM:TIGR04552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04552 family protein"
                     /protein_id="HPM46703.1"
                     /translation="MEPKNRIEKNFLKFPYELFNVVFSRKSFIDINRLNVHDEETALS
                     YMKNYGYDITDPLMKQKVVMILQESKNFIQNYLLEDPEGNDPALTVPPEVLFNDNILD
                     LLVMASEKDKSLFQTWACAVLRVAHTITHVENDLAKSFMPGIKKQIFSRFMDHLKMSR
                     TGEYYLGEGDQKIQLKMFEMKNEKSRESLILKLLHKKENVTADVFDRIGVRVVTYTKL
                     DVILVLKYFSVNHVVSFANIKPSRTRNNLINVERFLQGIEELNAHDLSSWKEEDVSEF
                     LERHLQLPPEEKEIETDRIIKENNLYSSTLYSSLQLTGRQLITIKDFFKPEINYRFFF
                     PFEIQILDEESFNESREGRASHEEYKKNQICAARKRVLNNVIKYQRQHLWKNDYQGGE
                     DEN"
     gene            2816..3562
                     /locus_tag="PL056_06525"
     CDS             2816..3562
                     /locus_tag="PL056_06525"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPM46704.1"
                     /translation="MKTVLQCCDGKAEIRQFKGTNSNMYIISYPDEKKSILVDCGLPW
                     DIPQLIEFIKVNDLPPVEKVVCTHFHVDHCSGWVELKKVFKSPLIHFHIDASPFVDGT
                     KTMDMPAFSDFKDIMIPVMKENQYMPELKEAIKTLYYGTTFKSRFPMDRVEFFNSEDN
                     LIPGFLTIHTPGHRPESVSFFDPSSGCFISGDFIIAMKGKIVVNTFVYDSEKQMESLE
                     KVKKLEDLMYLLPGHGEIKHFNEKLLKYIV"
     gene            complement(3577..4389)
                     /locus_tag="PL056_06530"
     CDS             complement(3577..4389)
                     /locus_tag="PL056_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46705.1"
                     /translation="MKKFSMLIAFLVLIFISCNEVEFSQNEYILENTLVCVKVDPPVA
                     TPGSKVTISVAVDDGKGEEPVVDISAGNMVFAGKTTAEFTIPDDVSTLFGDAAAAEMK
                     NEGFVDVEIGINIRNTEKNGVKMLRIAKAGYEPSNFGVNPDISKMEYEVVGSAQKIET
                     EKGSTISFEPSNIPEKVNLMPEKTVVNDVIKDDYIFSWFVSGTSTELPEIVEFDEDSG
                     QILVNFRDSSDAPLVGRYKFIAVLKPVKSLVGTDDAHYGSDFFSVTVDTDGK"
     gene            complement(4404..>6740)
                     /locus_tag="PL056_06535"
     CDS             complement(4404..>6740)
                     /locus_tag="PL056_06535"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPM46706.1"
                     /translation="IEEKKEEPAVAAPELVPINAGKKKPVKEKKAKPVEPAKPIEAKK
                     PVEPAKPAEAKKPIEPAKPAEAKKPVEPAKPAEVKKPAEPVKPAEVKKPAEPVKPAEV
                     KKPVEPAKPAEPVKPVEVKKPVEEAKPVEPVETQTVEEKKKKVEQYYFDSPQQLEDRE
                     SGIDITEPKKEVQKKLIRYNNENSTSKIPFVYNGLPSTAYGTGLIMGGNSSNENSYYI
                     DGVKVPFSHHMLTEKSVIDRNVLKSTYIYSGNFGPELGDSIGGVTNLSFKDPRTDRIG
                     GTFDLSMFGVSLMSEGPLAKSNYFSISFDFGSDDLFTRLAYDDENSIVSQGNLSGHIR
                     YLSEIDDKNSIVFTIAGARDNLYHLSTMKSKNVPVLSESLSPEIMFILAKGDYIYKTE
                     SINSRFTGSFVLSNFDYHVFGGDKLNTIDNRGTIEEHLSFKINEGNRFDLGVVFAAGI
                     FSTDTSFLLLPLEGEPGMIRSAGMLDQADNIGYIHPSLYLKYRFLAKGFEAVPGVYVS
                     GDFHNRDEWSGTIDPRLYLAYDITKWMKVYANGGMYSKRPQYDIAFHKLGNEALKHEK
                     AVHAKLGFAFEYEGFTADASGFYKYFYDLIRRNPEKITDYENTGTGWAAGTEIKAGYS
                     NKEVNGWVSYSFVKSLRTDGESAGERRADGDIPHIFKAALSYNFLKNWTVSGDISVAS
                     GTLMTGLAGTTYLADAGIYMPLYQEDDINGIRISNSVGYGFRIEYALFFKDFILGIYG
                     DIKGSNAKMDVVYNRDYSESGGLYLYPVLGTVGVKGDF"
     assembly_gap    6741..6840
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6841..7045)
                     /locus_tag="PL056_06540"
     CDS             complement(<6841..7045)
                     /locus_tag="PL056_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46707.1"
                     /translation="MKRIFILFLVLIFCIPLFLSAQKKKFAPEKTEEKEKVEKEEKKE
                     EKKEEKIEEKKEEPAVAAPELVPI"
     gene            complement(7056..10544)
                     /locus_tag="PL056_06545"
     CDS             complement(7056..10544)
                     /locus_tag="PL056_06545"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPM46708.1"
                     /translation="MKKFNFHINKNARERYSFKKGIFTICGNVIFPDYAAAKEFADRM
                     NRKFIEDGVKRYVRPGEIYAMGLIDEIMHYVFSVYRKNIDPLFLSKAYGSAVSNVGEK
                     SLQSTIDFFAGEFPPVPVESGEISGSEFLNGKIDDMPCMLIEFEEMILLKITNENPAT
                     SEFKELFDDSALIEKTEYLSAFSSIERSFDSQEKITVNGKKFSLIELLRAPFRASPDS
                     LSGQLLYMKENWGLELLKDFSDRLLVSVDIMKEETRPVFGQGGGHGGEVITKESLSSN
                     DWSYYDVETENFSVDSSWMPRVVMIAKNVFVWLDQLSKTYATNISTLDKIPDDELKSL
                     AKRGFTGLWLIGLWERSRASKRIKQIMGNHDAVASAYSLYDYSIAEELGGQHAFENFK
                     QRAWQFGIRIASDMVPNHMAIDSKWVNEHPEWFLSLDEPPFPGYTYNGEDLSSHPEIG
                     IYIEDHYFDKSDAAVTFKRVDKRNGDVKYVYHGNDGTCMPWNDTAQLNYLREDVRENV
                     ISTIIRIAKDFPIIRFDAAMTLAKRHYHRLWFPEPGKGGDIASRSERGMTRDEFNSFF
                     PKEFWREVVDRIAVEVPETLLLAEAFWLMEGYFVRTLGMHRVYNSAFMNFMKDEDNAK
                     YRESIRNILEFDPHILERHVNFLNNPDEETAVRQFGTGDKYFGVTLSMVTMPGLPMFG
                     HGQIEGYSEKYGMEYRKAYWNESVNWELVQRHEREIFPLMHRRNLFAHVEHFNLFDVY
                     RGDGSVDENVFAYSNGYGDKRVLVFYHNKYASTEGWIKTSAAYLDKSSGTLARKELHE
                     ALGICVREGKYLKFRDLITGLEYVISCKEICDNGFFVKLNAYKYYVFSDFTEVADSAL
                     EPWGELSAKLQGSGVEDLEFELDRIRFRSIHDLFRDFVNQETFEKFVEERLSVKKKSP
                     VSRFYKGLMDKYQKFINELSRFEGVEHINRDIAGLFINDLIAVIDLPKYKLKQYDIEA
                     DDYLHRNLSEDKYYLYMVLGFVTGARLSMIQKEKNDAKKSWELFIKYRLNLIFRELFE
                     STGLSSHHAMRIVRHIRAFIYYQNWWRQYEPEVLPLKLMEDVFRDDNMKSILQINEYE
                     GEIWFNKESFEDFTAGLFVISVLEILRNTEKVSERKIEFTKRYMVIKNLIAAAERSGY
                     KVNNFMQEVMQFGVTV"
     gene            complement(10559..10999)
                     /locus_tag="PL056_06550"
     CDS             complement(10559..10999)
                     /locus_tag="PL056_06550"
                     /inference="COORDINATES: protein motif:HMM:NF020977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HPM46709.1"
                     /translation="MISELKKAMMKAKMEHDTVKSNLLSTLVAEAAMVGKNDGNRATT
                     DQETINIIKKFIKNANENIKILDEMGKDKSAVIKEKEILESFLPKQLSPEELETIIDG
                     IAAALPERSVKMMGAVMAELKKKYDGQFDGRLASETVKKCLLKN"
BASE COUNT         2962 a         2539 c         1915 g         3554 t
ORIGIN      
        1 ctttcatcat tttcacctca atatgcctgc ttacaaactt ttctcaattt ctttaatcag
       61 ttcagggtgt ttttcacttt taaatattat tctgcctgtt ttgtcgagca tgaacatctg
      121 cggaaccact gctgaaccgt tattatctac tatgccgaat ttttttccgg ttatggaata
      181 tttgtcaaac actacgggaa atgagatctt ctgtttcttg aaaaactctt ttattttcac
      241 taattctgcc gatctgtctt cttcaaccgg aatgacgatg accacaaatt tgagtccttt
      301 cgctgaatac ttgcggtata cttcatccca ttttgaaatt gattttttac atggctcgca
      361 atgtattgaa aaaaaatcag cgaccatgat ctttgtgtca tttttttcaa gttccttctg
      421 agatgaatat aaaacatcat caagggtata aagatcaaat ctcttaacat cttcatcacc
      481 gctcatctgg aaagacagaa acacaactgc tatcatcaga aaaatccgtt tttccatatt
      541 caactccgta attggatcac tgtcaaaaat cttgatcccg attatacatt aaactttttg
      601 accttgcaaa tttaggaaaa ctgtttaacc cgcctttgaa aaggtttgag attacagaat
      661 atcaagtccg atcgtggctt tttcggacac acctatgaaa agggtcacct gaactccgtt
      721 tgactggaaa agttttttca cttcgctata gagaacattt ttcaattcct ctttatctgc
      781 aaacttatat tccccgatgt aggtatatcg gggaagatcg cgaagtgtgt caccttctgt
      841 tttgatcttg ctgtaaagtc cgatctcttc aagttctccg ttgaaaaaat catggttgta
      901 attttctgcc ttgagaactt cataacttaa aaaagcgaaa taatgcatgg ggttacctat
      961 tttgaagaaa gtttattcac tatagcaatt gcaaattttt cagcggcggc aggcccgttg
     1021 cctgttatta tgttcccgtc ttcaatcact tctccaccac tgaaaaccgc accgtatttt
     1081 tccatcacgg cacttgtttt cattccgtat tcagcatcat caccaagcca tactgtcgtc
     1141 ttctttcctt tgacagcacc ggcttttgca aggatcgtgg gagcccagca gatcgcaccg
     1201 agaacttcat gacctgcact gttcttagct atttcaaccg cccttttatc ggctctgaca
     1261 gaaggaacac cggctccacc gacaaaaaca actgcatcga aatctgattc attcacttca
     1321 tcaagcgtct ttttaacatc gaattttgca ccgaacatgc cggtcgcaac accttttttc
     1381 aagctcgcaa cagttactga agcaccttca tcaacaaatt tcttgaaagg aatttcaaac
     1441 tcttcatccc tgaacttctc cggcgcaatg atcataagga tctttttgct tttaattttc
     1501 atcttctcct ccttgataat cgtttttcca aagatgctgt ctttgatatt ttatgacatt
     1561 gttgagaact ctctttcttg cggcacatat ctgatttttc ttgtattctt catgacttgc
     1621 ccttccctct ctgctttcat tgaagctctc ttcatcaagg atctggatct caaacgggaa
     1681 gaaaaatctg tagtttatct ccggcttgaa aaaatctttt atggttataa gctgccttcc
     1741 tgtcaactgc aaagatgaat acaaagttga tgaataaagg ttgttctctt tgattatcct
     1801 gtctgtttct atctccttct cttccggcgg aagctgaaga tgcctttcaa ggaattcaga
     1861 aacatcctcc tctttccagc ttgaaagatc atgtgcgttc agctcttcta ttccttgaag
     1921 aaatctttcg acatttatca gattgttcct tgttctgctc ggcttgatgt ttgcaaaaga
     1981 gaccacatga ttgacagaaa aatatttcag gacaaggatg acatcaagct tggtataggt
     2041 cactaccctc acaccgatcc ggtcaaaaac atcagcagtc acattctctt ttttatgaag
     2101 aagtttaagt atcagacttt ccctgctttt ttcatttttc atctcaaaca tcttaagctg
     2161 gatcttctga tctccttcac ccagataata ttcccccgtt ctgctcattt taagatggtc
     2221 catgaatctt gagaatatct gtttttttat cccgggcatg aaactttttg caagatcatt
     2281 ttctacatga gttatcgtgt gggcaactct cagaacggca catgcccatg tctggaaaag
     2341 acttttatcc ttttctgaag ccattacaag aagatcgaga atgttgtcat tgaaaagcac
     2401 ttccggcgga actgtaagtg ccggatcatt tccttccgga tcttcaagaa ggtagttctg
     2461 aatgaaattt tttgattcct gaaggatcat caccaccttc tgcttcataa gaggatcagt
     2521 tatatcgtaa ccgtaattct tcatataact cagtgctgtt tcttcatcgt ggacattgag
     2581 cctgtttata tcaatgaagc ttttccttga aaacactaca ttgaacagct cataaggaaa
     2641 tttaagaaaa ttcttttcga ttctgttttt tggttccata tccgtttcct gaaaagatca
     2701 aaacgccatt ttacagaaaa taagcgttcc gacaactttt tggagattta tataaaccgc
     2761 tcttgcaaaa acagcaaaaa aaactatact ttcatttgtt ctggggagga aattcatgaa
     2821 aacagttctg caatgctgtg acggaaaagc tgagatcagg caattcaaag ggacgaacag
     2881 taacatgtat ataatcagtt atcctgatga gaaaaaaagc atccttgttg actgcggtct
     2941 tccgtgggac attccgcagt taattgaatt tattaaagta aatgatctgc cgccagtcga
     3001 gaaagttgtg tgcacccatt ttcatgtcga tcactgctcg ggctgggtcg aacttaaaaa
     3061 ggtttttaaa tcccctctta tacattttca tattgatgca tccccttttg tagacggaac
     3121 aaaaacgatg gatatgccgg cattttcaga tttcaaagat atcatgattc ctgtaatgaa
     3181 agagaaccag tatatgcctg aactgaaaga agcaataaaa acattatatt acggaacaac
     3241 atttaaaagc aggttcccga tggatagagt tgagttcttc aactccgaag acaacctgat
     3301 cccgggattt ctgacgattc acacacccgg gcacagacct gaatcggtca gcttttttga
     3361 tccatcgtca ggatgcttca tttcaggaga cttcataatt gcaatgaaag gaaagatcgt
     3421 cgtgaacact tttgtctatg atagtgaaaa gcagatggaa agtcttgaaa aagtaaagaa
     3481 actggaagac ttgatgtatc ttctgcccgg tcatggtgaa ataaaacatt tcaacgaaaa
     3541 actattgaaa tatatagtct aagaaaaccg gaagacctat ttgccgtccg tatcaacagt
     3601 cactgaaaag aaatcgcttc cgtaatgggc atcatctgtc ccgacgagac ttttgaccgg
     3661 tttcaaaacg gcgataaatt tatatcttcc taccagcgga gcatctgaag aatctctgaa
     3721 attaacaagt atctgccctg aatcctcatc aaattcaact atttccggaa gttcagtcga
     3781 agttcctgaa acgaaccatg aaaaaatata gtcgtctttt ataacatcgt taaccactgt
     3841 tttttcaggc atcaaattaa ccttttcagg tatgtttgac ggctcaaatg aaatagttga
     3901 ccctttttcc gtttctatct tctgagcact tcccaccact tcgtattcca tctttgaaat
     3961 gtcaggattc accccgaagt ttgacggctc atatcccgct ttggcaatcc tcaacatttt
     4021 cacaccgttc ttttcggtat ttcttatgtt tattccgatc tccacatcca caaacccctc
     4081 gtttttcatt tcagcggcag cagcatctcc gaaaagtgtt gacacatcat caggtattgt
     4141 gaactctgca gtagtctttc ctgcaaagac catgttgcct gcacttatat cgaccaccgg
     4201 ctcttcaccc ttgccgtcat caactgcaac tgaaatagtg accttgctcc ccggtgtggc
     4261 aacaggcgga tctactttta cacagaccag tgtattttca agaatatatt cgttctgtga
     4321 aaattccact tcgttgcacg aaataaaaat aagaaccaga aaagctatta acatactgaa
     4381 ttttttcatg atattcctcc tcatcagaaa tctcccttca cgccgacagt tcccaatacc
     4441 ggatagagat aaagcccgcc gctctccgag tagtcacggt tatagacgac atccattttt
     4501 gcattggaac cctttatgtc accgtatatc ccaagaatga aatccttaaa gaacagtgca
     4561 tattcaattc tgaaaccgta tccgacggaa ttgctgatcc ttatcccgtt tatgtcatct
     4621 tcctgatata aaggcatata tattcccgca tctgccagat atgtcgttcc ggcaagtcct
     4681 gtcatcaatg ttcccgatgc cacggaaatg tctcctgaaa ccgtccagtt cttgagaaaa
     4741 ttatatgaaa gcgccgcctt gaaaatatgg ggaatgtccc cgtcagccct tctttctccc
     4801 gctgattccc cgtcagttct caaactttta acaaacgaat aagataccca tccgttcact
     4861 tccttgtttg aataacctgc ttttatctcg gttcctgccg cccaaccagt gccggtgttt
     4921 tcataatctg tgatcttttc aggatttctt cttataagat cgtaaaaata tttataaaat
     4981 ccagaagcgt cagctgtaaa accttcgtat tcaaatgcaa atccgagttt tgcatgaacg
     5041 gctttttcgt gtttgagagc ttcgttaccg agcttatgga aagcaatgtc atactgcggt
     5101 ctttttgaat acattccgcc atttgcataa actttcatcc atttagtgat atcatacgca
     5161 agataaagtc gcgggtctat tgttcccgac cattcatctc tgttgtgaaa atctcccgac
     5221 acatatactc caggaactgc ctcaaacccc ttggccaaaa aacggtattt cagatatagc
     5281 gaagggtgta tgtaaccgat gttgtctgcc tgatcaagca ttccggcaga ccttatcatt
     5341 cccggttcac cttcgagggg aagaagaagg aaagaagtgt ctgtcgagaa tattcctgcg
     5401 gcaaagacta ctccgagatc gaatctgttt ccttcattga tcttaaagct cagatgctct
     5461 tctattgttc cgcggttgtc aatcgtattt aatttgtctc caccgaaaac atgataatca
     5521 aaatttgaaa ggacaaagct tcctgtaaac ctcgaattga ttgattctgt tttgtatatg
     5581 taatcccctt ttgcaaggat gaacattatc tccggggaaa gcgattcgct aagaacaggg
     5641 acatttttgc ttttcatagt tgaaagatga taaaggttgt ccctcgctcc ggcaatagtg
     5701 aaaactattg aatttttatc atcaatttca gatagatacc tgatgtgacc gctaagatta
     5761 ccctgagaaa ctatcgagtt ttcatcatca taggcaagtc ttgtgaaaag atcgtcagac
     5821 ccgaaatcaa atgagatgga aaaataattc gattttgcaa gaggtccttc tgacatcaat
     5881 gaaaccccga acatgctcag atcaaatgtt cctccaatcc tgtcagttct cgggtccttg
     5941 aaagacagat ttgtcactcc accaatcgag tcaccaagtt caggaccaaa atttcctgaa
     6001 taaatatagg tgcttttaag aacattccga tcgatcacac ttttttctgt cagcatgtga
     6061 tgggaaaagg gaaccttcac gccgtcaatg taataactgt tctcgtttga actgttaccg
     6121 cccattatta agccggttcc ataagcagtt gaaggaagtc cgttatatac aaacggaatt
     6181 ttagatgttg aattttcgtt attatatctg atcagttttt tctgaacttc ttttttcggt
     6241 tctgttatat ctattccgga ctctctatcc tcaagctgtt gcggagaatc aaagtaatac
     6301 tgctctactt tcttcttttt ttcctcaacc gtctgtgttt caaccggctc aactggcttt
     6361 gcttcttcaa cgggtttctt cacctcaacg ggttttaccg gttcagcggg ctttgccggt
     6421 tcaacgggtt tcttcacctc agcgggtttt accggttcag caggtttctt cacctcagcg
     6481 ggttttactg gttcagcagg tttcttcacc tcagcgggct ttgccggttc aacgggtttc
     6541 ttcgcctcag cgggctttgc cggttcaatg ggtttcttcg cctcagcggg ctttgccggt
     6601 tcaacgggtt tcttcgcctc aatgggcttt gccggttcaa cgggttttgc ctttttctcc
     6661 tttaccggtt tctttttgcc ggcatttatc ggaacaagtt caggcgctgc aaccgccggt
     6721 tcctctttct tttcttctat nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6841 ttatcggaac aagttcaggc gctgcaaccg ccggttcctc tttcttttct tctattttct
     6901 cttctttctt ctcttccttt ttttcttctt tctccacctt ctctttttct tcagtttttt
     6961 caggtgcgaa tttctttttc tgtgcactca gaaataaagg aatacaaaaa atcaggacca
     7021 gaaataaaat aaaaatcctt ttcatcggaa acctcctaaa cagtaacccc aaactgcatc
     7081 acttcctgca taaagttatt aaccttataa cctgatcttt ctgcagcggc aataaggttt
     7141 tttataacca tgtatctttt tgtgaactct attttccttt cagagacctt ttctgtgttt
     7201 cttagtattt caagcactga aataacaaaa agtccggccg tgaaatcttc aaaagattcc
     7261 ttgttgaacc agatctctcc ttcatattca tttatctgaa gtatcgattt catattatca
     7321 tccctgaaaa catcttccat taacttcaag ggaagaactt caggttcgta ttgtctccac
     7381 cagttctgat aataaatgaa agccctgatg tgtcttacga tcctcattgc atgatgtgaa
     7441 gataaaccgg tcgattcaaa aagttccctg aatatcaggt tcagccggta ttttatgaac
     7501 agttcccagc tcttttttgc atcattcttc tctttttgta tcattgaaag tctcgctccg
     7561 gtaacaaatc cgagcaccat atacaggtaa tatttgtctt ctgaaagatt tctgtgaaga
     7621 tagtcatcag cttcaatatc atattgcttc agtttatatt tcggaagatc tatcaccgct
     7681 atcaggtcat tgatgaaaag tcctgcaata tctctattga tatgctccac accttcaaat
     7741 ctgctgagtt cattgataaa tttctggtat ttatccataa gacctttgta aaatctggaa
     7801 acgggactct ttttctttac gctaagccgt tcctcaacaa atttttcaaa agtttcctga
     7861 tttacaaaat cacggaacag atcgtgaatc gaacggaacc ttatcctgtc gagctcaaat
     7921 tcaagatctt ccacacctga tccctgaagt tttgctgaaa gctctcccca tggctctaaa
     7981 gcagaatcag caacttcagt aaaatcgctg aaaacataat atttataggc atttaattta
     8041 acaaaaaaac cgttgtcgca aatttctttg cagctgataa catattccag tccagtgata
     8101 agatccctga atttcagata ttttccttct ctcacacata ttccgagcgc ctcatgaagc
     8161 tcttttcttg caagtgttcc tgaactttta tcaaggtacg ccgctgatgt ctttatccat
     8221 ccttctgtcg aagcatattt gttgtggtag aacaccagga ctcttttatc accgtaaccg
     8281 tttgaatatg cgaaaacatt ttcatcaaca cttccgtcac cgcgataaac atcgaacagg
     8341 ttgaaatgtt caacatgtgc aaaaaggttt cttctgtgca tcagaggaaa gatctctctt
     8401 tcatgccttt gaacaagttc ccagttgact gactcgttcc agtaagcttt tctatattcc
     8461 attccgtatt tttcagaata accctctatc tgtccgtggc cgaacatcgg cagtcccggc
     8521 attgtcacca ttgaaagtgt cactccgaaa tacttgtcac ccgttccgaa ctgtcttact
     8581 gccgtctctt cgtcaggatt attcagaaag ttcacatgtc tttcaagaat gtgcggatca
     8641 aattcaagaa tatttcttat tgattctcta tacttggcat tatcctcatc tttcatgaaa
     8701 ttcataaatg ccgaattata gaccctgtgc attccgagag ttcttacaaa atatccttcc
     8761 ataagccaga atgcttcggc aagaagaagt gtttcgggga cttcaacagc gatcctgtca
     8821 acaacctctc tccagaattc tttcggaaaa aatgaattga attcatctct tgtcattccc
     8881 cgttcagatc tggaagcaat atcgcccccc ttgcccggct caggaaacca cagacggtgg
     8941 tagtgtcttt tcgcaagtgt cattgcggca tcaaaacgga ttatcggaaa atcctttgct
     9001 atcctgatga tggtgctgat aacattttct cttacatctt cccgcagata gttaagctgc
     9061 gccgtatcat tccaaggcat gcatgtcccg tcattcccat gatagacata tttgacatct
     9121 ccgttccttt tatccaccct tttaaatgtg actgcggcat cggacttgtc aaaatagtgg
     9181 tcttctatat atatccctat ctccggatga ctcgaaagat cctctccgtt gtaggtgtag
     9241 cccgggaaag ggggctcatc cagagataga aaccattccg gatgttcgtt tacccatttt
     9301 gaatctatcg ccatgtgatt gggaaccata tcgcttgcga tccttatgcc gaactgccat
     9361 gctctctgct taaagttctc aaaagcatgc tgtcctccaa gttcttccgc aatggaataa
     9421 tcgtaaaggg aatatgctga agcgaccgca tcatgattgc ccatgatctg ctttatcctt
     9481 tttgaagcac ggctcctttc ccataacccg ataagccaca acccggtaaa cccccgtttt
     9541 gcaagggatt tcagctcgtc gtccggtatc ttgtcaagcg ttgaaatatt tgtcgcgtat
     9601 gtttttgaaa gctgatcgag ccagacaaag acattctttg cgatcataac aactctgggc
     9661 atccacgatg aatcaacaga aaaattttct gtttcaacat cataataact ccagtcattc
     9721 gatgaaaggg actctttcgt tatgacttct cctccatgac ccccgccctg accgaagaca
     9781 ggtctggtct cctctttcat tatgtcaaca gaaacaagaa gccggtctga aaaatccttt
     9841 aaaagctcaa gcccccagtt ttccttcata taaagcaact gccccgaaag tgagtcgggc
     9901 gatgccctga aaggtgctct caaaagttct atgagcgaaa atttctttcc gttcaccgtt
     9961 atcttttcct gtgaatcgaa cgatctttct attgatgaaa aagctgagag atattcggtt
    10021 ttttctatca gagctgaatc atcgaaaagt tccttgaact cacttgttgc gggattttca
    10081 tttgtaattt taagcagtat catctcttca aactctatga gcatgcacgg catatcgtct
    10141 atctttccgt ttagaaactc agaacccgag atctcgcctg actcaacagg aacaggagga
    10201 aactcaccgg caaaaaaatc tatggttgac tgaagtgact tctcccctac attggaaact
    10261 gcagatccat atgcctttga aagaaaaaga gggtcgatat tctttctgta gacactgaaa
    10321 acatagtgca ttatctcatc aataagcccc attgcatata tttcgcccgg cctgacatat
    10381 cttttgacgc cgtcttcaat gaacttccgg ttcatcctgt cagcaaattc ctttgccgcg
    10441 gcataatcag ggaagatcac attcccgcat atcgtaaata tccctttttt aaaagaatat
    10501 ctttctctgg cgttcttatt gatgtgaaaa ttgaattttt tcattaggat gtttccattc
    10561 agttttttaa aaggcatttt ttcactgttt cgcttgcaag tctaccatcg aactgaccgt
    10621 cgtatttctt tttaagctca gccatcaccg cacccatcat ttttacagat ctttcgggaa
    10681 gtgcagcggc gataccgtca attatcgttt caagttcttc agggctcaat tgtttcggga
    10741 gaaatgattc aagtatctcc ttttccttta tgacagcact tttgtctttc cccatttcat
    10801 caagaatttt tatgttctca ttggcattct taatgaattt tttaatgata ttaatggttt
    10861 cctgatcagt cgtcgccctg ttgccgtcat ttttccccac cattgccgcc tctgccacaa
    10921 gggttgaaag aaggttgctt ttgactgtgt catgttccat tttggctttc atcatcgcct
    10981 ttttaagttc gcttatcatt ttccacctct tagaatgaat gatatcttaa cggggaatta
    11041 tatctgttat aaattaaata tcaaattagc
//
LOCUS       DAOOYB010000085        10916 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_6443_length_10916_cov_9.111500, whole genome shotgun sequence.
ACCESSION   DAOOYB010000085 DAOOYB010000000
VERSION     DAOOYB010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10916
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6443_length_10916_cov_9.111500"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..893
                     /locus_tag="PL056_06555"
     CDS             <1..893
                     /locus_tag="PL056_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46710.1"
                     /translation="MSKVYFVDQFVTTDEDAIKTALDNTPGTAGGTECHWLVLYAATT
                     EDDIVGPCSTSEIMGTKTTCNIPKPDCSGKEGNRGGLCFRDKAMPILIMITDEDFTDN
                     KMPPVNELASTLAQKSLVEQNAKFIGIDSSSTSGSTAIKNKYESISQLTGTLDKNGKS
                     FNFSVGTDAVAGDGKPMSEKIAEAIESLTAFVQMDVWVAGDSDELCNDKSAAEFIIGG
                     IPIKADPPEGAGIDTANMKFTDVNPGTVVTFDVQFQNTFCPNYTDNPLLFKAQAMVLG
                     EGAYLSKKEVNVIVPKSDSK"
     gene            915..2603
                     /locus_tag="PL056_06560"
     CDS             915..2603
                     /locus_tag="PL056_06560"
                     /inference="COORDINATES: protein motif:HMM:NF039815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46711.1"
                     /translation="MKRSLFFLLFFFILAGCGESGNNNTGDTGNTGNTGDSGNSGNSG
                     DTGNTGDTGNTGDTGNSGNTGNTGNTEPVCPCGNDDEDDDKDGIKNGVEGCEDLDGDG
                     LPNCLDSDSDGDGYPDNTECPEQPCRDTDGDEIPDFLDKDSDNDGLPDKKEKEVGTDP
                     LDKDTDDDGTDDLAETVYGSDPTDPNSKVPANIFYVVLPYEAPDEVVRRLEFNTEIEK
                     VDAAIMIDLSGSMGEERQNLIDNIQTQIIGGIREVVEDSGFGLIHFMDLSPMSPLNQY
                     YGIDSFISTDAEKVKTAVGNLPDTTGASEPHYLVLHQASRGDGLTAKLKEDGDPIGFN
                     VNILPPDCSFHEGSIGGLCFRELALPIFIMITDEAMQDYIVANSNPQRILSVEAFEGM
                     NKINGKFIGVDSSGETGVVMNDYAVVSQATGSLDKNGDHFNFTIPSDGSVDMSAMIKD
                     AVVELTSFIQMDVWTDYSSEDECNGKSATEFFLFSKPIEADPADGVESFDDEKFYKVD
                     PGTKVTFDIQFHNDFCENRTDDYALYIAKIMVLGDGAFLSMREVQIIVPASQSK"
     gene            2687..4324
                     /locus_tag="PL056_06565"
     CDS             2687..4324
                     /locus_tag="PL056_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018247428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HPM46712.1"
                     /translation="MNSIYILVFSALWLIIAYFWYGKRVLQKKLVEPLDENPCPSHAS
                     NDGVDFYPAPSLVLFGHHFSSIAGAGPIVGPIVAVTFFGWGPAVIWLLVAGVFVGAVH
                     DYLSLIISVRHNGVSIPETAEKYVSKKARILFLIFVWLTIILVVAVFGNLAAKAMIAK
                     PELVLPTFLIIPLAIVFGMINKNGLLPLWANTAIALAGLIFLIWLGFQYPVNLPFDPK
                     TAYAIWFTLLMIYGFVASVTPVWILLQPRDYISSWVLIIGVALAFAGIFVVSPEMNAP
                     FIATVYSESQGPIIPFLFIIIACGAVSGFHSLIASGTSSKQIDKESDALFVGFGGMLV
                     ETIIGIICVIIAGGAIVWGTESGQLQEIFKSGGALAVYGTGFGKLTSFIFGSDVGSVI
                     GITIVNIFIMTTLDTSVRLSRFLTNEMIGGILPKPLKNKFSYTIIPIIPAFILGVTDS
                     WASIWPVFGAANQLVAALVFIILTSYLYSKGKPVKYTIYATVFMLLMTISALLWMVWK
                     FFFAKPDFLLGTVSAILIILAIMMVIEGVKKIRISRS"
     gene            complement(4321..5499)
                     /locus_tag="PL056_06570"
     CDS             complement(4321..5499)
                     /locus_tag="PL056_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46713.1"
                     /translation="MNRMILRNVSSGLFLLMIMISCGSQSSVEKYREDLKTFNNSVSK
                     IVENFDVISQKNGELNDLLNVDDLAGYGMNISLINSLLDETVNELDKLQKVTDGIDGE
                     NDSVPVAELRIEPVTMAGAALVVAGLAAFGKKCRDLAKKATDQWKKTSDTLADVADDK
                     ATEEDYKAEKNKLIETNQEFGYELSSRIITANLPGNPKNLGTLILKEVVTDQVQEGVK
                     TLFATQKCNDDIESTFCKVGAAVSNSDGKVPIFPEKSDVVIVKDGNARVVVEDVTVAT
                     GAEKEIVRAEIPVEDADPEIVTQNDAGTYDPTKTDIEGEGTTIMCYTFVDGVGHYGCL
                     KYNGSYSKLQNEDFDDAMAQCINADYKQYRAYFETDLACRKDCKAKAGEYGNISCDTP
                     "
     gene            complement(5593..6870)
                     /locus_tag="PL056_06575"
     CDS             complement(5593..6870)
                     /locus_tag="PL056_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46714.1"
                     /translation="MKYLTIIFLLFSLSLSATQRGEAILIGDTFVREHWSCSAANTTG
                     ASQSCPSGYSSYYSVGNYIGVPYKWGGFDSIAQFRDKISQGYGAGSYSSDGVLACVTG
                     VDCSGYVSRCWEQTTKYGTSTIQGISYELGSTDEMKKGDAFNKAGTHIVMLAYYHRDG
                     SPVIMEAAGGDYRKAVFRKVTWSYLNGYVPIRFDSISEDTNVTGTVSNPEIIDSFPYF
                     HSGNTRNMNSLEIDSYSAKPETMETGPEVIYQFTVSTSGTVDISVTDNQSEGIDNDIH
                     LLTTLNVDGSGMATDALARDDHNIVMHIDAGTYYIVVDSFSSSGVDKPGEYTMSASFE
                     HDPVIETDDDNEIPDVGVDQSVTPDDEITDNSEQPDNNSGNDDPVTPDDENVVSDNES
                     SSDDAQSVDDTEKTEDDHENVSEKSSGCSLSVL"
     gene            complement(6873..7703)
                     /locus_tag="PL056_06580"
     CDS             complement(6873..7703)
                     /locus_tag="PL056_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46715.1"
                     /translation="MKKMVFLLITFLVQLVFPGELVLDLKKENVNKLEKKATVLYGEK
                     DSEVGVFIAQKPDETSYGVSSFLVDKDVIYVIDSVNGKLKSAEISDFKFSSKINIKPN
                     TIDILKDKESIYLYNFQNSEFLKVGTQNLVPVIRKKTTSPIFKPFSNLTNEIGKKVDD
                     VVSVSKNSVKTATISVLKEKHTLVLKNSDLGSLQFISSDGAGNLHFVVEIIKSMNPIK
                     VERYWLKTDNGFKLLNISNLPISDIFLPFREIQVNSDGTIWFINPAINGLEILFGKGR
                     "
     gene            7863..9461
                     /locus_tag="PL056_06585"
     CDS             7863..9461
                     /locus_tag="PL056_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46716.1"
                     /translation="MKFNFFVTVILSVIITACATASNTAESDSVPTMTKSANAQNYGD
                     EAPKVWTPLTQIEMGALIKAKMDPDNESQMVLSLFFTGDIREGEEIGASIGAYRTFIE
                     LCDTELAKIKDAKEKGKKLHELFFKQFIESPRKKDAPSSGIFGLLTKKEYDANTAALL
                     FAIIAQKYGFTSEISFTKGIVEEIVNQKTGLSIKIFSGKAYLTLKHKEMYSPAQVIPF
                     LEDGFDPYINLSFFENMHEKNPSEFEDAQTELGKYYKRMPLTFEKALLFQYKIDKLNE
                     TAETENPPMNRRIEMAAHLTDLCDIHLDRIRAWRNTYPFILQKKIPGEMFSFIDTINS
                     ELNRTAELCKDEPDFEDLAWDLFLFSAFEYSDSLDGVKLKTALFNGYRFLSTSSKDYE
                     KKKVFLANSLSHYLNKIISSDVIEKELATVKDVIAAIPVTEIRTGSASSFYYQSGEHY
                     LAKKDLWRAGQFYAECSFVYGGQYQKICIQKGVDSLYKYAQTCLGRGVCATASDAVSK
                     CMEKIPDKKECQKIKDLYDQQCGK"
     gene            complement(9471..10241)
                     /locus_tag="PL056_06590"
     CDS             complement(9471..10241)
                     /locus_tag="PL056_06590"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015175441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPM46717.1"
                     /translation="MEKKQWYESLFENYGKTYDNECFTQGTIGECDFLETEFNFDKSL
                     KILDVGCGTGRHAIELTKRGYNVTGIDLSESQLERAREKATEAGFDIKFLKADARNLP
                     FNEEFDAAIMLCEGGFPLMETDEMNFEILKNVSRSLNKNGKLIFTTLNGLFPLYHSVE
                     QFCDSAKGEGNATYRGNTFDLMTFRDHNITELVDDLGNKKTLDCNERYYVPSEITWLL
                     KTLGFSKIEIFGAKLGAFSRSDKLTTEDFEMLVIGEKG"
     gene            complement(10266..>10916)
                     /locus_tag="PL056_06595"
     CDS             complement(10266..>10916)
                     /locus_tag="PL056_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46718.1"
                     /translation="CATNQKKEVDELKKEIMRLQDDIREMKELLKESRPKETPEKKVE
                     KPQPVEPVELPPQEPTLNPQPKKIEIVKPVTTEEKETVHYYNPRMGKNKVSVRITPWK
                     DGKRKMYLYDPYGNVTFEREDVRMSYSDTATIKKFHDNGAVAVLYIHMNPGASMYWYE
                     TTITFSINNEPEWKTSLQQPATLEDHLNSKYYWDKKTKTWKKQEIIREQPVPKEYQ"
BASE COUNT         3096 a         2386 c         2155 g         3279 t
ORIGIN      
        1 atatgtcaaa agtatacttt gttgatcaat ttgttacaac agatgaagat gcaatcaaga
       61 ctgctcttga caacactcct ggaacggcag gtggaactga atgtcactgg cttgtccttt
      121 atgccgctac aacagaagat gatattgtag gtccgtgctc aacatcagaa ataatgggaa
      181 caaaaacaac ctgcaatatc ccgaaacctg actgctcagg caaagaagga aaccgcggag
      241 gactttgttt cagagataag gcgatgccta ttctcataat gataactgac gaagatttta
      301 cagacaataa aatgcctccg gtaaatgaac ttgcttcaac gcttgcccag aaatctcttg
      361 ttgaacagaa cgctaaattc atcggaattg actcgtcaag cacaagcgga agtacagcga
      421 taaagaacaa atatgaaagt atctctcagc ttaccggaac tcttgataaa aatggtaaaa
      481 gttttaattt ctcagtagga actgatgcgg ttgcaggtga cggcaaaccg atgagcgaaa
      541 agatcgctga ggcaattgaa tcactcaccg ctttcgttca gatggatgta tgggtggcag
      601 gtgattcgga tgaactttgc aatgacaaaa gtgccgctga attcataatc ggcggaattc
      661 cgataaaagc cgatccgcct gaaggagcag gaattgacac agcaaacatg aagttcacag
      721 atgtcaaccc cggaactgtt gtcacattcg atgtacagtt ccagaacaca ttctgcccca
      781 actacactga caatcctctc ctcttcaaag ctcaggcaat ggttcttggt gaaggtgcat
      841 atctttccaa aaaagaagtc aatgtgatcg ttccgaaatc cgacagcaag taattcaaaa
      901 taatggagaa ttccatgaaa agatctttgt ttttcctttt attttttttc attttagctg
      961 gatgcggtga aagcggaaac aacaacaccg gtgataccgg gaatactgga aacacgggtg
     1021 attccggcaa cagcggcaac tctggtgata ccggcaatac aggagacaca ggaaacaccg
     1081 gagatacagg aaacagcggc aatactggca atacgggcaa caccgaaccg gtctgtccct
     1141 gcggcaatga cgatgaagat gatgacaagg acggaattaa gaacggtgtc gaaggttgtg
     1201 aagaccttga cggtgacgga ctgccaaact gtcttgactc agattcagat ggtgacggat
     1261 acccggacaa cactgaatgc cctgaacaac cgtgcagaga taccgacggt gacgaaatcc
     1321 ccgattttct tgacaaagac agcgacaacg acggacttcc cgacaaaaag gaaaaagaag
     1381 taggcacaga tccgttagac aaggatacag atgatgacgg aaccgatgac cttgcggaaa
     1441 ctgtctacgg ctctgacccc accgacccga acagcaaggt gcctgcaaat attttttatg
     1501 tcgttcttcc ttatgaggca cccgatgaag ttgtcagaag attggaattt aatacagaga
     1561 tcgaaaaggt tgatgcggca ataatgatag acctttccgg ttcaatgggc gaagaaaggc
     1621 agaatctgat cgacaatatt cagacacaga taatcggcgg cataagggaa gttgttgaag
     1681 acagcggttt cgggcttata cacttcatgg acttgagtcc gatgtcacca ttaaatcagt
     1741 attacggcat agacagtttc atttccaccg atgctgaaaa agtcaaaact gctgtaggaa
     1801 atcttcccga cacaacaggt gcatctgaac cccattatct tgttcttcat caggcatcaa
     1861 gaggcgacgg actgaccgct aaactgaagg aggacggcga tccaatcggt tttaatgtca
     1921 atatacttcc gcctgactgc tcattccacg aaggaagcat cggtggactc tgtttccgtg
     1981 aacttgccct gccgatattt attatgataa cagatgaagc aatgcaggat tacatagttg
     2041 ccaactcaaa tccacaaaga attctgagtg ttgaagcatt tgaaggaatg aataaaatta
     2101 acggaaagtt cataggtgtt gattcttcag gggaaacagg tgttgtcatg aacgattatg
     2161 cagtcgtcag ccaggcaacc ggctcacttg acaagaacgg agaccacttt aatttcacaa
     2221 taccatcgga cggttcagtt gacatgagcg caatgataaa ggacgccgtt gtcgaactca
     2281 caagcttcat tcagatggat gtctggacag actacagctc cgaagacgaa tgcaacggca
     2341 aaagcgcaac agagttcttc cttttctcaa aaccgataga agcagatccg gcagatggcg
     2401 ttgaaagctt tgatgatgaa aaattctata aagtcgatcc cggaacaaaa gtcacattcg
     2461 acatccagtt tcacaacgat ttctgtgaga acagaacaga cgattatgcc ctttacatcg
     2521 ccaagatcat ggttttaggc gacggcgcat ttttaagcat gagagaagtc cagataatag
     2581 ttcccgcttc acagagcaag taaggtctta aattatctaa aaacagtttg aaatccttat
     2641 tcatcggagt attatctctc ccggattaac atttccggag ttcgttatga atagtattta
     2701 cattcttgtg ttttcggctt tgtggctgat catcgcctat ttctggtatg gaaagagggt
     2761 tctgcagaaa aagcttgttg agccgctcga tgaaaacccg tgtccgagcc atgcttcaaa
     2821 tgacggtgtt gatttttatc ctgctccgtc attagtcctt ttcgggcatc acttttcatc
     2881 aattgcgggg gctggtccaa ttgtcggtcc gatcgttgcg gtgacttttt tcggctgggg
     2941 tcctgcagtt atctggcttc tggttgccgg ggtttttgtg ggagctgttc atgactatct
     3001 ttcgctcatt atttcagtgc gtcacaatgg agtgagcatt cctgaaacgg cggaaaaata
     3061 tgtttcaaaa aaagcccgca tcctttttct gatctttgtc tggcttacga taatccttgt
     3121 tgtagcagtt ttcggcaatc ttgcggcaaa agcaatgatc gcaaagccgg aacttgttct
     3181 accgactttt cttataatcc ctcttgcaat agtttttgga atgatcaata aaaacggtct
     3241 attgcctctc tgggcaaata ctgctatcgc tcttgccgga ctgatatttc tcatctggct
     3301 cgggtttcaa tatccggtga atctgccatt tgatccgaaa acggcttatg ctatctggtt
     3361 cacgctttta atgatatacg gatttgtcgc ttcggtaact cctgtctgga tccttcttca
     3421 gccgagagac tatatttcaa gctgggttct tattatcgga gttgcacttg cctttgccgg
     3481 aatttttgtc gtaagtcccg aaatgaacgc ccctttcatt gcaacggtgt acagtgaatc
     3541 tcaaggccct atcataccgt ttttattcat aattattgcc tgcggagcag tttcgggatt
     3601 tcattcgctt attgcttcag gaacatcttc aaagcagata gataaagaaa gcgatgccct
     3661 ttttgtcggc tttggcggaa tgcttgtcga aaccataatc ggaataatct gtgtcatcat
     3721 tgcaggcgga gcaatagtct ggggcacaga aagcggtcaa ttgcaggaga ttttcaagtc
     3781 aggaggagca cttgcggtct atggaactgg tttcggcaaa cttacctctt tcattttcgg
     3841 atctgatgtc ggcagtgtca tcggtataac gatcgtgaac attttcataa tgactacgct
     3901 tgacaccagt gtccgtctct caagatttct cacaaatgaa atgatcggcg gcatcttgcc
     3961 gaaaccttta aaaaacaagt tctcatatac gataatcccg ataattccag cgtttattct
     4021 gggagttaca gattcatggg caagcatctg gcctgttttc ggagcggcaa atcagcttgt
     4081 cgcggcactt gttttcatca ttctcacttc atatctgtat tcaaaaggaa aaccggtgaa
     4141 atacacgata tatgcaactg ttttcatgct tctgatgaca atatctgcac ttttgtggat
     4201 ggtctggaaa ttcttttttg caaaacccga ctttcttctt ggaacagttt ctgctatact
     4261 tatcatcctt gcgataatga tggtgattga aggggttaaa aagataagaa tatcaagatc
     4321 ttaaggtgtg tcgcaactga tattcccgta ttctcctgct ttggctttgc agtccttccg
     4381 gcaggcgaga tcagtttcaa aatatgcgcg gtactgttta taatcagcat tgatgcactg
     4441 agccattgca tcatcaaaat cttcattctg gagttttgag tatgaaccgt tgtatttgag
     4501 gcagccataa tgaccgacac catcaacaaa agtataacac atgatcgttg tcccctcacc
     4561 ttcgatatca gtttttgtgg gatcataagt cccggcatca ttctgtgtca ctatttcggg
     4621 gtcagcatct tcaacgggaa tttcagctct gacaatttct ttctctgctc ctgttgcaac
     4681 tgtcacatct tcaacgacaa ctcttgcatt cccgtcctta acgatcacaa catccgattt
     4741 ttcaggaaaa atagggactt tgccatcgct atttgaaacg gcagcaccga ctttacagaa
     4801 agtgctctcg atgtcgtcat tacatttttg ggttgcaaaa agggttttca ccccttcctg
     4861 aacttgatcg gtaacaactt ctttcaatat cagtgttccc agatttttcg gatttccggg
     4921 aagattggca gttatgatcc ggcttgaaag ttcatatccg aattcctgat ttgtttcaat
     4981 gagtttattc ttttctgctt tataatcctc ttctgtcgcc ttatcatccg ctacatcggc
     5041 aagagtatca cttgtctttt tccactgatc tgttgctttt tttgcaagat ccctgcattt
     5101 ttttccaaaa gcggcaagtc ctgcgacaac aagagcggct ccggccatcg tgaccggctc
     5161 tattctcagc tcagccacag gcacagaatc attttcaccg tcaatcccgt cagtcacttt
     5221 ctgcaactta tccagttcgt tgactgtctc atcaagaagc gaatttatga gagaaatgtt
     5281 catcccgtaa cccgccagat catcaacatt aagaagatcg ttcaattcac cgttcttttg
     5341 agatatcaca tcaaaattct caacgatctt tgacaccgaa ttgttgaaag ttttaagatc
     5401 ttcacggtat ttctccacac tgctttgaga accgcaacta atcataatca ttagtagaaa
     5461 caacccagac gaaacatttc ttaaaatcat tctattcatc gtcacctcca caaatatcgc
     5521 aatattttat ctcttatcac cctttttaca agaaacttgc aactccaaca aaaatagcaa
     5581 tcaaatccta atttacaaca ccgacaacga gcatcccgag cttttttctg agacattttc
     5641 gtgatcatct tctgtttttt ctgtgtcatc aacggactgt gcatcatcag aagagctttc
     5701 attgtcactc acgacatttt catcatcggg cgttaccgga tcatcatttc cgctgttgtt
     5761 gtcgggttgt tcagaattat cagtgatttc atcatcaggt gttacactct ggtcaactcc
     5821 aacatcaggt atttcgttgt catcatccgt ctcaattaca gggtcatgct caaaagatgc
     5881 gctcattgtg tattcacccg gtttatcaac tcctgaagaa gagaatgaat caacgacaat
     5941 ataataagtt ccggcgtcaa tatgcatcac aatgttgtga tcatcccttg caagtgcatc
     6001 tgtcgccatt ccggagccgt caacattaag agttgtaaga agatgaatgt cgttgtcgat
     6061 cccttcgctc tggttatccg ttacagatat gtcaacagtt cctgaagtac tgacggtgaa
     6121 ttgataaata acttccggtc ctgtttccat tgtttcgggt ttagctgaat aactgtcaat
     6181 ttcaagagag ttcatatttc ttgtgtttcc gctgtgaaaa tagggaaacg aatcaataat
     6241 ttcagggttt gaaactgtcc ctgttacatt tgtgtcctca gaaatactgt cgaatcttat
     6301 cggaacatat ccgttgaggt aactccatgt aacttttctg aaaaccgcct ttctgtaatc
     6361 tcctccagcc gcttccatta ttaccggaga accgtctcta tgatagtagg caagcataac
     6421 aatatgcgtt cccgccttgt taaaagcatc tccttttttc atttcatcgg ttgatccgag
     6481 ttcataagaa attccctgaa tagttgaagt tccgtatttt gttgtctgtt cccagcatct
     6541 tgaaacatag ccggagcagt caacacctgt aacacatgcc agaactccgt cagaagaata
     6601 tgaaccggct ccgtaacctt gtgatatctt gtcacggaac tgtgcaattg aatcaaatcc
     6661 gccccattta tatggaactc cgatataatt tccgactgag tagtaacttg aatatcctga
     6721 tggacagctt tgagatgcac ccgtagtatt tgccgctgaa caactccagt gttctcttac
     6781 gaatgtatca ccgataagta tcgcctcacc tctttgggtt gcggacaatg ataacgaaaa
     6841 aaggagaaaa ataatggtta aatatttcat tttcacctcc cttttccaaa aagaatctct
     6901 aacccgttaa ttgcaggatt tatgaaccat atcgttccat ctgaattaac ctggatctct
     6961 ctgaacggaa gaaaaatgtc actaatcgga agatttgaaa tattcaacaa tttaaaaccg
     7021 ttatcagtct taagccagta tctttcaact tttatagggt tcatgctttt tatgatctca
     7081 acaacaaagt gaaggtttcc tgcaccgtca cttgaaatga actgtaacga gccgagatca
     7141 ctgtttttaa gaacaagtgt gtgcttttcc tttaaaactg agatcgttgc ggttttcaca
     7201 ctgttttttg aaacagaaac gacatcatcc acctttttac cgatctcatt agtcagattt
     7261 gaaaatggct taaatatcgg tgatgtggtc ttttttctga taacgggaac gagattttga
     7321 gttccgactt ttaaaaattc actgttttga aaattataaa gatagatgct ttccttatcc
     7381 tttaaaatat caatcgtatt tggtttgata ttgatttttg agctgaactt aaaatcagaa
     7441 atttctgcac ttttaagctt tccattaacg ctgtcaataa catatataac atctttatca
     7501 acaagaaatg aagaaacgcc gtaacttgtt tcatcgggct tttgagcaat aaaaacaccg
     7561 acttctgagt ctttttcccc gtaaagaacg gttgctttct tttcgagctt attcacattt
     7621 tcttttttca ggtcaagcac caattcccct ggaaaaacca gctggacaag aaatgtaatt
     7681 aataaaaaga ccattttttt catgactttc tccacaatca gttttaaagc acgcaaaata
     7741 ttttagttta atatgtgatt tttgcaaata acattcagag ctgataatcc ccttttattt
     7801 gcttttaaat atctttactg ttatgacaaa actgttgatg gtttatgtgt gttgaggttg
     7861 ttatgaaatt taattttttt gttacagtta ttctttcagt aattattact gcctgtgcta
     7921 ctgcatcaaa tactgctgaa tcagacagtg tccctacgat gacaaaatcg gccaatgctc
     7981 agaattacgg agatgaggca ccaaaagtat ggactccgct aacccagatt gaaatgggcg
     8041 cacttataaa ggcaaaaatg gaccctgaca atgaatcaca gatggttctg tcgcttttct
     8101 ttacaggcga tataagagaa ggtgaagaga tcggtgcatc aataggtgcc tacagaactt
     8161 ttattgaact ttgtgacact gaacttgcaa aaataaagga tgcgaaggaa aaaggaaaaa
     8221 aacttcacga acttttcttt aaacagttca tagaaagtcc cagaaaaaaa gatgctccga
     8281 gctccggcat tttcggactt cttacaaaaa aggaatatga cgcaaatact gcggctctcc
     8341 tttttgcaat aattgcacag aaatacggat tcacttctga aatatcattt acgaaaggta
     8401 ttgtcgaaga gatcgttaac cagaaaacag gactttcaat aaaaattttc tcaggcaagg
     8461 cataccttac tctcaaacat aaggaaatgt actctcctgc tcaggtaata ccttttcttg
     8521 aggatggatt tgacccctac attaatctca gtttctttga aaacatgcac gaaaagaacc
     8581 catcggaatt tgaagatgct cagaccgaac ttggaaaata ttataaaaga atgcctctca
     8641 cttttgaaaa ggcgcttctg ttccagtata agatagacaa gctgaatgaa acggcagaaa
     8701 ctgaaaaccc gccaatgaac agaaggatcg agatggcggc tcacctgaca gatctgtgtg
     8761 acattcacct tgacaggata agggcttgga gaaacacata tccgttcata cttcaaaaaa
     8821 agatccccgg agagatgttt tcgttcattg acacaattaa ctctgaactt aacagaaccg
     8881 cggaactctg caaagatgag ccggattttg aagaccttgc ctgggacctt ttcctttttt
     8941 ctgcatttga atattcagat tcacttgacg gtgttaaact gaaaactgca cttttcaatg
     9001 gatacagatt cttatccact tcgtcaaaag attatgaaaa aaagaaggtg ttccttgcaa
     9061 actccctcag tcactacctc aacaagataa tcagttcaga tgtaattgag aaagaacttg
     9121 caacagtaaa ggatgtcatt gccgccatcc ctgtgacaga aatacggaca ggatccgctt
     9181 catcttttta ttatcagtca ggagaacact atctggcaaa aaaagatctc tggcgtgcag
     9241 gacagttcta tgctgaatgt agctttgtct atggcggtca ataccagaag atctgcattc
     9301 agaaaggagt tgattcgctt tataaatatg ctcagacctg tcttggaaga ggagtctgtg
     9361 ccaccgcctc agacgcagtc agtaaatgta tggaaaaaat tcccgataag aaagaatgcc
     9421 agaaaataaa agatctttac gaccagcaat gcgggaaata aataacttat tcagcccttc
     9481 tcccctatca caagcatttc aaaatcttct gtggtgagtt tatcgcttct gctgaatgca
     9541 ccgagttttg cgccgaagat ctctattttg ctgaagccga gagtttttaa gagccaggtt
     9601 atttcgcttg ggacatagta tctttcgttg cagtcgagtg tctttttgtt gccgagatca
     9661 tcaacaagtt cggtaatgtt gtggtcacgg aatgtcatca gatcgaatgt gttgcctctg
     9721 tatgttgcgt tcccctcccc ttttgcactg tcgcagaact gttcaacaga atgataaagc
     9781 ggaaaaagtc cgttaagtgt cgtgaaaatc aacttcccgt ttttattaag cgatcttgag
     9841 acatttttca gtatctcaaa attcatttca tctgtctcca tcagcggaaa acccccttcg
     9901 cacaacatta ttgcagcatc aaactcctcg ttaaacggaa ggtttcttgc atcagctttc
     9961 aagaatttta tatcaaatcc ggcttctgtt gctttttccc ttgccctttc aagctgagat
    10021 tcagacagat caattcctgt cacattgtat cccctttttg taagctcgat tgcatgacga
    10081 cccgttccgc agccgacatc cagtattttc aatgacttat caaagttaaa ctcagtttcc
    10141 agaaaatcac actcgccgat tgttccctgc gtgaaacatt cgttgtcata agtttttcca
    10201 taattttcaa aaagtgattc ataccattgt ttcttttcca taaaatctcc cctaatctat
    10261 tgtttttact gatattcttt cggaaccggc tgttctctta tgatttcctg ctttttccat
    10321 gtttttgttt tcttatccca ataatatttg ctgtttaagt gatcttccag tgtagccggc
    10381 tgctgaagag aagttttcca ttccggttca ttgttgatgc tgaaagtaat tgttgtttca
    10441 taccagtaca tacttgcacc cgggttcatg tggatataaa gaaccgcaac cgctccgttg
    10501 tcgtggaatt tctttattgt cgccgtgtca ctgtaactca ttctcacatc ttccctttca
    10561 aaagtgacat ttccataagg atcgtacaga tacatttttc tctttccatc tttccacgga
    10621 gtgatccgca ccgaaacctt atttttcccc attcgcggat tgtaataatg aaccgtctct
    10681 ttttcttcag ttgtgaccgg ctttacaatt tcaatttttt ttggctgagg attcaatgtc
    10741 ggctcctgag gcggcagttc aaccggctca accggttggg gcttttcaac cttcttctcc
    10801 ggcgtttcct taggtctgct ctctttcaaa agctccttca tctccctgat atcatcctga
    10861 agccgcatta tctccttttt tagctcatca acctcttttt tctgattcgt tgcaca
//
LOCUS       DAOOYB010000086        10795 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_6551_length_10795_cov_11.466108, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000086 DAOOYB010000000
VERSION     DAOOYB010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10795
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6551_length_10795_cov_11.466108"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /locus_tag="PL056_06600"
     CDS             complement(<1..854)
                     /locus_tag="PL056_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HPM46719.1"
                     /translation="MAGNELIQIDAIKSRIFTIRGMQVMLDSDLAELYGVETKNLNLA
                     VKRNIERFPNTFRFQLAEEEYKVLRLQIETSNEKRGGRRYAPFVFTEQGVAMLSAVLR
                     SETAIKVSIRIMEAFVSMRHFILENEQISKRFEIIEKRQMEYKIETDSRIEKVLSALE
                     SKQIQPKQGIFFDGQVFDAHKFVSDIVRSAEKSIILIDNFVDDTVLTLFSKRKQGVTL
                     KILTKNLSKQIQLDMQKFNEQFPPAEIIEFNHFHDRFMIIDNHDLYLFGASLKDLGRK
                     WFGFAKMD"
     gene            complement(1352..2671)
                     /locus_tag="PL056_06605"
     CDS             complement(1352..2671)
                     /locus_tag="PL056_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46720.1"
                     /translation="MKGIATVLILSGICFSVYASSLKKENAKITADRKMIADLEKGLS
                     KDERGCEKPSEGLDKSVLIELQIYCNARNSAKRRLVPLKDHISAYETAVTNYYANCKI
                     VDTDELFVLCEALDDKVVKAAELVNDEKESLRKDLDEMENSAKKAAIINKEKNDWSKK
                     EHDQQINDSLRVKRDLVMTMQKGLIDDSVILEKSRIKFSSALNNSSGEIRDRTEYLLK
                     GVTSLISVNKNLRDTCQSMTRNIDITNSSCTIKYSEKACTEAENKLTQVFDEGNAKLK
                     AYTGEKERLRTLEMELHKLSIAEIAVKMENANRQISFYIGNLQLQNDQLKQRIELSGN
                     DLEVLKGMDGKAFVETYLGYVETMKSLEKESAGFIDFLGLSTARINEFIKNCTQNKSE
                     YSTECRIEGDNIVKDVDSIMKKISQYGAKFSRLNSDLAAFAKKVSGK"
     gene            2774..3223
                     /gene="dtd"
                     /locus_tag="PL056_06610"
     CDS             2774..3223
                     /gene="dtd"
                     /locus_tag="PL056_06610"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HPM46721.1"
                     /translation="MISVIQRVKSGSVSVDGSVISSINKGLLVLAGIEKGDTFADLEY
                     SAKKITELRIFEDDAEKMNLSVIDVGGEILAVSQFTLLGNVQKGRRPGFDNAMSPEIA
                     KTMFDDFVKMLSKAISTVKTGSFGAHMEVSLINDGPVTFVIDSRKRV"
     gene            3293..4108
                     /locus_tag="PL056_06615"
     CDS             3293..4108
                     /locus_tag="PL056_06615"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HPM46722.1"
                     /translation="MRIFNQTLYIAVICAALLCSCLKSESSVPAFDRGGTEKSKIGVR
                     GFGSENNEQTAPEVSERRLVKEGSVGFQTDSITETTPKIRQMITQYKGFISREDAYNY
                     TGSRSFTLIARIPVDSFDKFIEELEHIAGSFDSRSIGVKDITAQFVDSEARLKAKKEI
                     EKNYLAILEKAKTINETLAVQKLLGEIRGEIESVEGQLKLMQDQTAYSTLTISYYEQK
                     ESVFSFVRNIVNALKDGWVGFLQVLIAAAYAWVFIVIVATFLIFRKLRKNRKK"
     gene            4306..5400
                     /locus_tag="PL056_06620"
     CDS             4306..5400
                     /locus_tag="PL056_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004078264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPM46723.1"
                     /translation="MKLDQIKIKGFKSIKEADIELGDLNVLIGPNGAGKTNFISLFKL
                     LNKMIEGNLQKHITQGSGAENFLFFGSKTTDRIELFLKFANNGYEAKFYQDNSDRLIF
                     EEEKCYFQGPEYKKPYKEVIGTNHSETALLEIMKIPKMTVGNHVVENLKSWKYYHFHD
                     TSDSAKVKKMCQINDNMYLRTDASNLAAFLYLMQEKHHENYEAIRDTVRLVAPFFDNF
                     ILRINPFNEEMIRLEWREKGSDFPFLANQLSDGTLRFICLATVLLQPKPQLPSTIIID
                     EPELGLHPYAIKVLASLMKSASKHTQIIVSTQSVPLVNEFEPENLIVVDKIENASVFK
                     KVTSKELSSWLEEYTLGELWEKNVLGGRPQ"
     gene            5397..6071
                     /locus_tag="PL056_06625"
     CDS             5397..6071
                     /locus_tag="PL056_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012593984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4276 family protein"
                     /protein_id="HPM46724.1"
                     /translation="MIRVNVIAEGQTEETFVRTVLAPELGTKNVFLTARCVETSRSKS
                     RIYRGGLLDYEKAKKDILRWLKQDKEAVVTTMFDLYALPDSFPGKKENKKITDPYKLV
                     SILEERFFEDINSETECEKRFIPYIQLHEFEGLLFSDVEKIHEVMKVKTDERSKLVEI
                     SEKFNNPELINQGSDTAPSKRLSNLYPGYDKITNGAIIASKIGLSEIRRKCSHFNEWL
                     SKTESL"
     gene            6091..6369
                     /locus_tag="PL056_06630"
     CDS             6091..6369
                     /locus_tag="PL056_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46725.1"
                     /translation="MRIFLAVMVFLCAFALSARDYGKCSFNSDCNPNVKCNSGVCADA
                     AGGKCSFASDCGFKGAKCNSGKCSNAPDGKCSFSSECPSGKCDSGKCK"
     gene            6411..7055
                     /locus_tag="PL056_06635"
     CDS             6411..7055
                     /locus_tag="PL056_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46726.1"
                     /translation="MKIINKCKLLTVFLFVGFFCSGLYAEDFKKHTLGISVEGGVSRS
                     FLNSYASNGKAWHDIYGTFGGTFFYQYRFTKVVAVRPGLGIYSYFFGVPGPMDDLKDE
                     VFATIHGRIDLSLALYTYTGNKVAFPFLITPFISMRFYDSAGTYETYFEGEKEVMHNF
                     RTFIGGQISFGIESSNPEKTGAGFHIFMRMNDVMGTGGQSGVPCSLGGSLMLFW"
     gene            7062..7613
                     /locus_tag="PL056_06640"
     CDS             7062..7613
                     /locus_tag="PL056_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014473453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HPM46727.1"
                     /translation="MKKLKHTIVFAMLFPAFFAGCASNKMPELKTVEKVDLSRYLGLW
                     YEIARIDQTFQKGCVATTAEYSLKDDGRIKVLNKCRKNTIDGKEKSAEGVAWVVDKET
                     NAKLKVSFFWPFSGKYWVIDLEPEYKWAVVGDPSREYLWILSRSPEMDEETYEGIMKR
                     LKEQLYDISKIQKYPQIKSDKKN"
     gene            7679..7888
                     /locus_tag="PL056_06645"
     CDS             7679..7888
                     /locus_tag="PL056_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006965610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HPM46728.1"
                     /translation="MRTTLDLPEKLLEKAMKAANIKTKTQVIVQALEELIRRHKIAEL
                     KNFKGKVDLELDLDCVRSRDEDYSG"
     gene            7869..8267
                     /locus_tag="PL056_06650"
     CDS             7869..8267
                     /locus_tag="PL056_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015680194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HPM46729.1"
                     /translation="MKIIVDSSVWIDYFRNGQNSGILDNLIDENLIAVNDLILTEIIP
                     PLQLKKQTRIIDLLNEIENLELHIDWQQIRDFQYICLKKGINKVGISDLIIAQNAIQN
                     NTSIFTLDKHFSLLKTVIGVSVFDQKTLQI"
     gene            complement(8356..10011)
                     /locus_tag="PL056_06655"
     CDS             complement(8356..10011)
                     /locus_tag="PL056_06655"
                     /inference="COORDINATES: protein motif:HMM:NF012632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46730.1"
                     /translation="MKKILFLTVTVLLFSVSFSCVTDEKEIFDDSELTNDSDQGNSGN
                     TGNTGDSGDSGNSGDTGDSGDSGDSGNSGNSGNTGNSGDSGNSGDSGNSGNTGSYGDN
                     TPEPETETVKMVSGGTSHVCAIKESGHLYCWGQGNRGQLGNNQTPGGDNPNPMPLKVG
                     MDAWQSICAMADATCGVKKNKESFCWGHNADGQIANGLDGYTFQATGTPSPILLGADQ
                     NQYAPLFCGGSTILAYNNDDLWGWGSNRMGTLANGQVGYDASYPGGSATAYVPRLLNE
                     NKDNWPKFTNISISTVHACGISDEKELFCWGSYDMGQFGDGRRGDGFDKYSYRSQPLY
                     SEYEKPVKINEFKWIDVASLSQNTCAVKDDGTLWCWGIAEWGIIGIGDVFASYTAGTP
                     QDNATYTEAFQQLVTNGQCEEQFYSEDKAASQKRRYPPVCTKPVQVGTASNWVSVSGS
                     SNHVCAVNSSNELFCWGENEYGQIGNGEKGDVYSTLPSNYDLVTSPYKIEGEFIAVSA
                     GYNFTCAISLDKKLYCWGFSALGIPGFTGNKDNPQTTPVKISF"
     gene            10197..>10795
                     /locus_tag="PL056_06660"
     CDS             10197..>10795
                     /locus_tag="PL056_06660"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HPM46731.1"
                     /translation="MKVISNLKFKELLEKAKLKEGTFLIFAAILIGIAAGCAFLFFQW
                     LINYLSVLFIGENTNNSIAGFKRLPLHFKILLPLTGVFISGFIIRYISRESGGAGIGF
                     LLKVMKVKNGIMPPMMTFFKIVTSALSIATGVPLGTEGPIIIIGSAVGSTVGQFFNMS
                     ISRIKLFVGCGAAAGLAVAFNAPIAGTILAVETILGNYA"
BASE COUNT         3246 a         2057 c         2194 g         3298 t
ORIGIN      
        1 atatccattt ttgcaaatcc gaaccatttt ctgccgaggt ctttgagtga tgctccgaaa
       61 agatagaggt cgtgattatc aattatcatg aagcggtcgt ggaagtgatt gaattcaatt
      121 atttcagcag gtgggaactg ttcattgaat ttttgcatgt cgagttgaat ctgttttgaa
      181 aggtttttag tgagaatttt gagcgtaact ccttgtttcc gttttgaaaa aagagtgaga
      241 acggtgtcat caacgaaatt gtcaatgaga atgatcgatt tttcagctga acggacaata
      301 tctgagacaa atttatgtgc gtcaaaaacc tgaccgtcaa aaaagattcc ttgctttggc
      361 tgaatttgct ttgattcaag ggccgacaaa actttttcaa ttctactatc ggtttctatt
      421 ttatattcca tctgcctttt ctcaataatt tcaaaccttt tagatatctg ctcattttcc
      481 aatataaaat gtcgcattga aacaaaagct tccattattc ttatacttac tttaatagca
      541 gtttcactcc tcagaacagc agaaagcata gcaactccct gttctgtaaa aacgaatggt
      601 gcgtatcgtc ttcctccccg tttttcgttt gaggtttcaa tttgcaacct caaaactttg
      661 tactcttctt ctgcgagttg aaatctgaat gtgtttggaa atctttcaat atttcttttt
      721 acagctaaat tgaggttttt tgtttcaaca ccatagagtt cagccaaatc actgtcgagc
      781 atcacctgca tcccgcgaat tgtgaaaatt ctgcttttaa tcgcatcaat ctgtattagc
      841 tcatttcctg ccattttcca actccaagag tttatttgcc gatagataat gcaataatca
      901 aattaaatat gcaagaaaac agataagatt atctaactcg aagaaaaaca ggaaattatg
      961 aattgaatta tgcgcctggt ttattccttt aattgctaat tgctttaatt gacaagcttc
     1021 tgctgaatca tataatttta ctggattttt taaatagtga gagggaccaa aatgaaaaaa
     1081 gttttaatta ttatcgcatc ggtgattctg gttttaatta ttggcggatt cacaaccgca
     1141 aaagctctgg aaaaaaaaga gtaactacaa tatcttaaga aaattcgtgc aaattgaaag
     1201 ttgtacttgc aatttacata aattgttgca atattccgat attattctct tttctaaggt
     1261 gtggacataa cctttcggaa taaataaaaa taaagaaata tttgccggga aaaaagtagg
     1321 acttgtaatt tcaggtggaa atgttgagag attatttccc cgaaactttc tttgcaaaag
     1381 cggcaagatc gctgttgaga cggctgaatt tcgccccgta ttgagagatc tttttcataa
     1441 tggaatcaac atctttgact atgttgtctc cttcgatcct gcattcagtt gaatattcac
     1501 ttttattttg cgtacagttt tttatgaatt cgtttattct tgcggtgctg agacctaaaa
     1561 agtcaataaa tccagcactt tccttttcaa gtgatttcat cgtttcaaca tatccaagat
     1621 aagtttccac aaatgctttt ccatccattc ccttgagcac ttcaagatcg tttccgctga
     1681 gctcaatcct ctgtttaagc tgatcgttct gaagctgaag attcccgatg taaaacgaaa
     1741 tctgacggtt cgcattttcc attttgacag caatttcagc aatagagagt ttatgaagct
     1801 ccatttcaag agttctgagt cgttcttttt cgccggtata tgccttgagt ttggcatttc
     1861 cttcatcaaa tacctgagtg agcttatttt cagcttctgt acacgccttc tcagaatatt
     1921 ttatagtgca tgaactgttt gtaatgtcta tgttccgtgt cattgactgg caggtatcac
     1981 gcagattttt gttgactgaa ataagtgaag taaccccttt gagcagatat tcagttctat
     2041 ctctgatctc tcctgaactg ttattgagtg cggacgagaa tttaattctg gatttttcaa
     2101 gaattaccga gtcatcaata agccctttct gcatggtcat cacaaggtct cttttgactc
     2161 tgagagaatc gtttatctgc tgatcgtgct ctttttttga ccagtcgttc ttttctttat
     2221 tgatgattgc cgcttttttt gccgaatttt ccatttcatc aagatctttt cttaaacttt
     2281 ccttttcatc atttacgagt tcagcggctt ttaccacttt gtcgtcaagg gcttcgcaaa
     2341 gtacgaacaa ctcatctgtg tcaacaattt tgcagtttgc ataatagttt gttacagcgg
     2401 tttcataggc ggaaatgtga tcttttaaag gcacgagccg tctttttgcg gaatttcttg
     2461 cattgcaata tatctgaagt tcgatcagga cgcttttatc aagtccttcc gatggttttt
     2521 cgcagcccct ttcatctttt gaaagtcctt tttcaaggtc ggcgatcatt ttgcggtcgg
     2581 cagttatttt tgcattttct tttttgagcg aactcgcata aactgaaaaa catattccgg
     2641 ataagatcag aactgttgca atccctttca attttttact ccatcaaatc agcgcatata
     2701 ttttaatgat aagggaaaaa tagacaattt tttgatgatt gttagaattg acaagttttt
     2761 ttaggagttg tctatgatct cagtcattca gagggtgaaa agcggatcgg tttcagttga
     2821 tggaagtgtc atttcttcaa taaacaaagg gcttttagtc cttgcaggaa ttgaaaaagg
     2881 tgatactttt gcagatcttg aatattcagc aaaaaagata actgaattaa ggatatttga
     2941 agatgatgca gagaaaatga acctttcagt cattgatgta ggtggagaaa ttcttgccgt
     3001 cagccagttc acacttttag gcaatgtgca gaaaggtcgc agacccggat ttgacaacgc
     3061 aatgtctcct gaaatcgcca aaacgatgtt cgatgatttt gtaaaaatgc tctccaaagc
     3121 aatatcaaca gtaaaaacag gtagttttgg cgctcacatg gaagtgtcac ttattaatga
     3181 cggtccagtc acattcgtca tagattcaag aaaaagagtt taggaaagtt tgataaaaaa
     3241 ctgatttagc ttattatttc caagttgttt aaatcttttt caggaggaga tcatgagaat
     3301 ttttaatcaa actctttata tcgcggtgat ttgtgccgct ctgctttgtt catgtttaaa
     3361 aagtgagtcg tccgttccgg catttgatcg tggcggtact gagaaatcaa agatcggtgt
     3421 caggggcttc ggctctgaaa ataatgagca aaccgcccct gaggtttctg aacgcagact
     3481 tgtgaaagag ggaagtgtcg gttttcagac tgattcgata acggaaacaa ctccgaagat
     3541 ccgccagatg ataactcaat ataaagggtt tatttctcgt gaagatgcct acaactatac
     3601 tggaagcaga agttttactc ttatcgcccg cattcctgtt gattcgtttg acaagtttat
     3661 tgaagagctt gagcatatcg caggttcttt tgattcgaga tctatcggag taaaggacat
     3721 cacggctcag tttgttgaca gcgaagcccg tctcaaagcg aaaaaagaga ttgaaaagaa
     3781 ctatcttgcc attcttgaaa aagcgaaaac gataaatgag acacttgcag tccagaaact
     3841 gcttggcgag atccgcggtg aaattgagtc tgttgaagga cagctcaagc taatgcagga
     3901 tcagacggct tacagcacac tcacaatttc gtattacgaa cagaaagaat cggtattctc
     3961 attcgtcaga aacatcgtaa acgcattgaa agacggatgg gtcggattcc tgcaagtgct
     4021 catcgccgca gcttacgcct gggtcttcat cgtcatagtc gcaacatttt tgatattcag
     4081 aaagctgaga aaaaatagaa agaaatagaa gcaaatgtta agaaaaatct tcaaactcga
     4141 tgaaccgctt ggaagagcgg gatttatttc tgtttcagta accatttctt tgttttttct
     4201 tttaagctga tatattttct ggtgaataat cacggtattt agaaagtttg ataaaataac
     4261 aggtttagtt tattattcca cagagttttt atcaaaggag gatgtatgaa gcttgatcaa
     4321 ataaagatta aagggtttaa atctataaaa gaagctgata ttgaactcgg agatttgaat
     4381 gttcttatcg gtccaaacgg agccgggaaa accaacttca tttctctttt taagctgtta
     4441 aacaagatga tagaaggcaa ccttcaaaaa catatcaccc aaggaagtgg tgcagaaaac
     4501 tttctttttt tcggttcaaa aaccactgat cgaatagaac tgtttcttaa atttgctaac
     4561 aatggatatg aagctaaatt ttatcaggat aattccgaca ggcttatatt tgaagaagaa
     4621 aagtgctatt ttcaaggacc ggaatataaa aaaccatata aagaagtaat tggtacaaat
     4681 cattcagaaa ctgcattgct tgaaattatg aaaattccta aaatgacagt aggaaaccat
     4741 gttgttgaaa atctaaagag ctggaaatat taccattttc acgacacaag tgattcagcc
     4801 aaagtgaaaa agatgtgcca gataaatgat aatatgtatc ttcggactga tgcatcaaac
     4861 cttgctgcgt tcctttatct gatgcaggaa aagcaccatg aaaattatga agcgatcaga
     4921 gacactgtca gattggtcgc tccttttttt gataatttca ttcttcgcat taaccctttt
     4981 aatgaagaaa tgatccggct tgagtggcgt gaaaagggct ctgattttcc atttcttgca
     5041 aatcagcttt cggacggaac tttgagattc atctgtcttg ctacagttct tcttcaacct
     5101 aaacctcaac ttccttccac aataattatt gacgaacctg agctcgggct tcatccttat
     5161 gcgatcaaag tgcttgcctc tttaatgaaa tcggcttcaa aacacacaca gataatagtt
     5221 tcaactcagt cagtaccgct tgtgaatgaa tttgagccgg aaaatttaat agttgtcgat
     5281 aagattgaaa atgcaagtgt gtttaaaaaa gtgacgagca aagagctttc aagctggctt
     5341 gaggaataca ctctcggcga actctgggaa aagaatgttc tcggaggcag accgcaatga
     5401 taagagtaaa tgtcatagca gaaggacaga ctgaagaaac ttttgtcaga acagtactcg
     5461 ctcctgaact aggaacgaaa aatgtttttc tgaccgcaag atgtgtggaa acatcaagaa
     5521 gcaaatcaag aatatatcgt ggcggtttac ttgattatga aaaagcaaaa aaggatatat
     5581 taagatggtt gaagcaggat aaagaggcag ttgtaactac catgtttgat ttgtatgcac
     5641 tgcccgattc ttttccaggg aagaaggaaa acaaaaaaat cacagatcct tacaaactag
     5701 tttctattct tgaagaacgg ttttttgaag acataaattc tgaaacagag tgtgaaaaaa
     5761 gattcattcc ttatattcaa ctgcatgaat ttgaaggact gctgttctca gatgttgaaa
     5821 aaattcacga agttatgaaa gtgaaaaccg atgaaagatc gaaacttgtt gaaatatcag
     5881 aaaaatttaa caatcctgag ctgataaatc aaggatcaga cacagcacct tcaaaaagac
     5941 tttcaaacct ttatccgggt tatgataaga tcacaaatgg agccataatc gcttcaaaaa
     6001 taggactcag tgaaattcgc agaaaatgca gtcactttaa tgaatggctt agcaaaacag
     6061 agagtttgta atcttttttt ggaggggatc atgagaattt ttttagcagt tatggttttt
     6121 ttgtgtgctt tcgctctttc cgcccgtgat tacggcaaat gcagtttcaa ttccgactgt
     6181 aatccgaatg taaagtgtaa cagcggagta tgtgcagacg cagccggtgg caaatgttct
     6241 tttgcttcag actgtggctt caaaggtgca aaatgcaatt cgggcaaatg ctcaaacgca
     6301 cctgacggca aatgttcctt cagctcagaa tgcccaagcg gaaaatgcga ttcaggaaaa
     6361 tgtaagtaga atcagaaatg agtgaaaatt aaaaaaaaca gggcaccgta atgaaaataa
     6421 ttaataaatg caaattgttg acagtgttct tgtttgtcgg ctttttctgt tcaggtctat
     6481 atgcagaaga ttttaagaag cacacattgg gcatttcagt agagggtggt gtttcaagaa
     6541 gttttttaaa ttcttatgca agcaatggaa aggcctggca tgatatttac ggaactttcg
     6601 gtggaacttt tttctatcag tacagattta caaaagttgt tgccgtaagg cctggattgg
     6661 gcatttattc gtattttttt ggtgttccgg gcccgatgga tgaccttaag gatgaagttt
     6721 ttgctacaat tcatggcagg attgatctca gccttgctct ttacacctat acagggaaca
     6781 aagttgcttt tccgtttctt ataacgccgt ttatttcaat gaggttttat gactcagcag
     6841 gaacatatga aacctatttt gaaggagaaa aagaagtcat gcataacttt agaactttca
     6901 tcggcggaca gatctctttc ggaatagaaa gttcaaaccc ggaaaaaaca ggtgcaggat
     6961 ttcatatttt tatgcggatg aatgatgtta tgggaacagg cggacaatcc ggtgttcctt
     7021 gttctctggg cggatcttta atgttgttct ggtagaatga aatgaaaaaa ttaaaacata
     7081 cgattgtttt tgcgatgctt tttccggcat ttttcgccgg gtgcgcttca aataagatgc
     7141 cggagctcaa aactgtcgaa aaggttgatc tgagccgtta tctcggtttg tggtatgaga
     7201 tcgcccgcat tgatcagact tttcagaaag gatgtgtggc gacaactgct gaatattcgc
     7261 tgaaagatga cggcagaata aaagtgctca acaaatgccg caaaaacacc attgacggaa
     7321 aggaaaaatc ggcagaaggt gtcgcatggg tcgttgacaa ggaaacaaac gcaaaactga
     7381 aagtcagttt tttctggccg tttagcggaa agtactgggt gatagatctt gaacccgaat
     7441 ataaatgggc tgtcgtcggt gatccaagcc gtgaatatct ctggatactt tcaagatctc
     7501 ccgaaatgga tgaagaaact tatgaaggaa taatgaaacg gcttaaagaa cagctttatg
     7561 atatctcaaa aatccaaaaa tatccgcaga tcaaatcaga taaaaaaaat tagaaagcga
     7621 ttttccacct tgacttttat gcataaaaac ataaaatcat gcagatggag gtggtgaaat
     7681 gagaacaaca ttagatttgc ctgaaaaatt gcttgaaaaa gcaatgaaag ctgcaaacat
     7741 caagactaaa actcaagtta ttgtccaagc ccttgaagag cttatcagaa gacacaaaat
     7801 tgctgaactg aaaaatttca agggaaaagt tgatcttgag ttggatcttg attgtgtcag
     7861 gagcagagat gaagattata gtggatagtt cggtctggat cgattatttc aggaacggac
     7921 agaattccgg catactcgat aatctcattg atgaaaacct gatagctgtg aacgacctta
     7981 tcctgactga gataattccc cctttgcagt taaaaaaaca gacacggata attgatctgc
     8041 tcaatgagat agaaaacttg gaacttcata ttgactggca gcagataagg gattttcaat
     8101 acatttgtct gaaaaaaggc atcaacaaag tgggaatctc cgaccttata attgctcaga
     8161 acgccattca gaacaacaca tcaatcttta cactggataa acatttcagt ctgctgaaaa
     8221 ctgtaatcgg tgtaagtgtt tttgatcaga aaactcttca aatttaactt aaaacttatt
     8281 cttgtctgtg tattagttac cctgattcga ggtaactatt tcaaattagt tccctcgttt
     8341 gaggataact ttgcgttaaa aactgatctt cacgggtgtg gtctgagggt tatccttgtt
     8401 gccggtgaat ccggggatgc cgagtgctga aaagccccag caatacagct ttttatcaag
     8461 tgatatggcg catgtaaagt tgtaaccggc tgatactgct atgaattcgc cttcaatttt
     8521 atatggtgaa gtaacaaggt cgtagtttga gggaagtgtt gaatagacat cacctttttc
     8581 gccgtttccg atctgtccgt attcattttc accccagcag aaaagttcat tgctgctgtt
     8641 tactgcacag acatgatttg acgagccgga aactgaaacc cagtttgaag cggttcccac
     8701 ctgcacaggt tttgtgcaga ccggtggata tctccttttc tgagatgccg ctttatcttc
     8761 agaataaaac tgctcttcac attgaccgtt tgttacaagt tgctgaaatg cttcagtata
     8821 tgtggcgtta tcctgcggag ttccggcagt atagcttgca aaaacatcgc cgatcccgat
     8881 gatgccccat tcagcaattc cccagcacca taaagtgccg tcatctttga ccgcgcaagt
     8941 gttctggctc agagacgcga catctatcca tttgaattca ttgattttaa cgggcttttc
     9001 gtattcgctg taaagtggct gagagcggta tgaatacttg tcaaaaccat ccccccttct
     9061 tccatcaccg aactgcccca tatcataact tccccagcag aaaagctctt tttcatctga
     9121 aatgccgcag gcgtgcacag tagatattga aatatttgtg aatttcggcc agttatcctt
     9181 gttttcatta agtaatctag gcacataggc agttgcgctt ccacccggat aagaagcgtc
     9241 gtaaccaacc tgaccgttgg caagagttcc cattctgttg cttccccaac cccagagatc
     9301 atcattgttg taggcaagaa ttgtgcttcc tccgcagaaa agcggtgcat actgattctg
     9361 atctgcacca agaaggatcg gagaaggagt cccggtcgct tgaaaagtgt atccgtcaag
     9421 cccgttagct atctggccgt cagcgttgtg gccccagcag aacgattctt tattcttttt
     9481 tactccgcag gtcgcatcag ccatagcaca gatactctgc caagcatcca ttcccacttt
     9541 cagcggcatc ggattaggat tgtctccacc cggagtttga ttgtttccaa gctgtccgcg
     9601 gtttccctga ccccagcagt agagatgacc gctctctttt attgcacaga catggcttgt
     9661 tccgcctgaa accattttga ctgtctcggt ttccggctcg ggagtgttgt cgccgtaact
     9721 gcccgtatta cctgagtttc ccgagtcgcc tgagttgcca gaatcccctg aatttcctgt
     9781 gttgccggaa ttcccagagt tcccggaatc gcctgaatct cctgagtcac cggtatcgcc
     9841 tgaattaccg gaatcgcctg aatcaccagt gttccctgtg tttccggagt taccctgatc
     9901 agagtcattt gtcagttcac tgtcgtcaaa gatctctttt tcatcagtga cacatgaaaa
     9961 tgaaacagaa aaaagcagaa cagtaaccgt taaaaaaaga atttttttca tagccccacc
    10021 atttaaaaaa aacaacactt caaacaatgc ttaaatatca tcaattatcg tctttttcca
    10081 gaaagtcggt cagaaaagaa ccttattggt ggtacgattt caccaatccg cactttttat
    10141 ttacaagaca gtataaaaag tgtatatgaa aaacgctaaa agttttgttg agaaaaatga
    10201 aagtcatttc aaatctaaaa tttaaagaac ttcttgaaaa agcgaaactc aaggaaggaa
    10261 ctttcctcat tttcgcagca attctgatcg gtattgcggc aggatgtgcg ttccttttct
    10321 tccaatggct tatcaactat ctttcagttc ttttcatcgg ggagaatacg aacaacagca
    10381 tagcaggttt taaaagactt ccccttcatt ttaagattct tcttcccctg actggtgttt
    10441 tcatcagcgg attcatcata agatacatca gcagagagag tggcggagca ggcataggat
    10501 tccttcttaa agtgatgaaa gtcaaaaacg gaataatgcc gccgatgatg acatttttta
    10561 aaatagtgac atcggctctt tcaatagcaa caggagtgcc gctcggaact gaagggccca
    10621 taatcattat cggttcggca gtcggatcca ctgtgggtca gttcttcaac atgtcgatat
    10681 cgagaataaa actttttgtg gggtgcggag ccgccgcagg tcttgccgtt gcattcaatg
    10741 ctccgattgc cggaacaata cttgcagttg agacaatcct tggaaattat gcgat
//
LOCUS       DAOOYB010000087        10792 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_6553_length_10792_cov_8.919903, whole genome shotgun sequence.
ACCESSION   DAOOYB010000087 DAOOYB010000000
VERSION     DAOOYB010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10792
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6553_length_10792_cov_8.919903"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..382
                     /gene="rpsJ"
                     /locus_tag="PL056_06665"
     CDS             74..382
                     /gene="rpsJ"
                     /locus_tag="PL056_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HPM46732.1"
                     /translation="MSDKIRIKLKAFDHKILDQSVSEIIETVSRSGARIAGPIPLPTK
                     IHKYCVLRSPHVDKKSREQFEMRIHRRILDILNPTNQTMDELMKTDLSAGVEIEIKTY
                     "
     gene            425..1045
                     /gene="rplC"
                     /locus_tag="PL056_06670"
     CDS             425..1045
                     /gene="rplC"
                     /locus_tag="PL056_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HPM46733.1"
                     /translation="MAAGLIGRKLGMTQIFREDGKILPVTVIEAGPCYVIQKKETEKD
                     GYTAIQVGFEKKEKNVKKPEAGHFKNAAKGVFRKLKEFRVAANEIGSYELGSELTVTM
                     FKPGEKIKVTGFSKGRGFAGVVKRWSFGGGRTTHGSKFHRKGGSIGQNVCPGKVWKGK
                     KMPGRYGNEQITVKNLEIVRVVPADNTIVVKGAIPGSTGGIVYIKK"
     gene            1057..1686
                     /gene="rplD"
                     /locus_tag="PL056_06675"
     CDS             1057..1686
                     /gene="rplD"
                     /locus_tag="PL056_06675"
                     /inference="COORDINATES: protein motif:HMM:NF012782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HPM46734.1"
                     /translation="MPVVDLFDRSKKKVGEIELKDAVFAVPFEEKKVLMYEVIKMQTA
                     SWRSGTHSTKTYATISGGNSKPWKQKGTGRARRGTMRASTLRGGAPAFGPQPRDYSYT
                     IPKKKRIAAMRALLSERLSKGNLFVVKAFDFDQIKTKEAVSVLKGNWDLKEAVIVGGD
                     SEEKFLLSVRNIQEYLFLHKTQINLYDIMAYKSVIFTEEAIKYIDEVLG"
     gene            1686..1985
                     /locus_tag="PL056_06680"
     CDS             1686..1985
                     /locus_tag="PL056_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005545486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HPM46735.1"
                     /translation="MKKEKHFDIIKRPLLTEKSAVLSKDQKQSYVFEVKLDSTKEDVK
                     EAVEKVFNVKVDSVNTVVVGGKTKRVGRTLGKRSTWKKAYVTLKEGHQINLVEGL"
     gene            2018..2848
                     /gene="rplB"
                     /locus_tag="PL056_06685"
     CDS             2018..2848
                     /gene="rplB"
                     /locus_tag="PL056_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HPM46736.1"
                     /translation="MGIKIFKPTSNGRRNMTGSDYSEITKSKPEKSLVEPIRKSGGRN
                     NTGRITIRHRGGGNKTMYRIIDFKRNKFDVPGKVEAIEYDPNRNARIALIFYLDGEKR
                     YIIAPDKINVGDVIVSAGEKEADIKPGNSMKIKFIPVGTFIHNVELKIGKGGQIARAA
                     AAGVQVLGKEGDHATVRMPSGEIRIVHLECRASVGTVGNQEISNIKIGKAGKQRWLGK
                     RPHVRGVVMNPVDHPHGGGEGKTSGGRHPVTPWGKPTKGYRTRNNKRTDKFRVRRRDK
                     "
     gene            2881..3147
                     /gene="rpsS"
                     /locus_tag="PL056_06690"
     CDS             2881..3147
                     /gene="rpsS"
                     /locus_tag="PL056_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HPM46737.1"
                     /translation="MPRSVKKGPFVDSHVWKKVIKAVDGGDKKPIKTWSRRSTIIPEM
                     VGLTFSVYNGQKFIPVFVTEDMVGFKLGEFSPTRTFRSHSGSKK"
     gene            3217..3561
                     /gene="rplV"
                     /locus_tag="PL056_06695"
     CDS             3217..3561
                     /gene="rplV"
                     /locus_tag="PL056_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HPM46738.1"
                     /translation="MEDKIVRAQLRNFRMSASKVRLVADIIRGKSAGDAKNILEFTPR
                     KAAMALKKLLDSAIANAENNYNLDPDTLFLKTVMVDEGQVMKRFVPRAQGRAAKILKR
                     SSHVTIELAANK"
     gene            3587..4255
                     /gene="rpsC"
                     /locus_tag="PL056_06700"
     CDS             3587..4255
                     /gene="rpsC"
                     /locus_tag="PL056_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HPM46739.1"
                     /translation="MGQKVNPIGLRLGVTRTWESVWYSHKNYSKWLHEDLKVRKAIKK
                     EFYPAGIAKIDIERSGEKLKIIIHTAKPGIVVGRKGVGIEKIKEMVQKIAKDVTDIFV
                     QTEEVRKPEIDAQLVSENVAEQLERRAHFRRAMKRAISQAMKSGAEGIKIQTSGRLGG
                     AEIARTERYQEGRTPLHTLRANIDYGYAIAKTTYGVIGVRVWMSKGDSIDKKPENKRK
                     QKKN"
     gene            4273..4692
                     /gene="rplP"
                     /locus_tag="PL056_06705"
     CDS             4273..4692
                     /gene="rplP"
                     /locus_tag="PL056_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HPM46740.1"
                     /translation="MLMPNKVKHRKVMKGKRSGLATSCNSIDFGEFALKATECGWINS
                     RQIEAARVAIMRAVKRGGRMWIRIFPDKPITKKPLEVRMGKGKGNPEEWVCVIKPGRI
                     LFELGDVDSETALEAFRLASHKLPIRTKIIEKGDELK"
     gene            4689..4895
                     /gene="rpmC"
                     /locus_tag="PL056_06710"
     CDS             4689..4895
                     /gene="rpmC"
                     /locus_tag="PL056_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003247579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HPM46741.1"
                     /translation="MKVSELREKTLAELQEKLLSYKEEQFNLRMQKGMGQMENNSRAV
                     SIKKDIARIKTMITEKKKAVEVKK"
     gene            4892..5149
                     /gene="rpsQ"
                     /locus_tag="PL056_06715"
     CDS             4892..5149
                     /gene="rpsQ"
                     /locus_tag="PL056_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002642288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HPM46742.1"
                     /translation="MTEKIKRTLTGTVVSDAMDKTVAVQVTRRFKHALYKKMITARKK
                     FLAHDEQNQYKVGDIVKIQECAPMSKRKTWFVKEKIGESRR"
     gene            5152..5520
                     /gene="rplN"
                     /locus_tag="PL056_06720"
     CDS             5152..5520
                     /gene="rplN"
                     /locus_tag="PL056_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011567679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HPM46743.1"
                     /translation="MIQEMTRLAVADNSGAKEVQCIKVYGGSKIRYAGVGDVIVVAVK
                     VAEPKGKVKKGDVKKAVIVRQAKEIRRDDGSYIRFDDNAVVIIDDAYEPIGTRVFGPV
                     ARELRGKGFMKIVSLAPEVL"
     gene            5531..5842
                     /gene="rplX"
                     /locus_tag="PL056_06725"
     CDS             5531..5842
                     /gene="rplX"
                     /locus_tag="PL056_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007009524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HPM46744.1"
                     /translation="MANIKKNDTVKVIAGKDKGKSGKVLKVLVKDEKVVVEGVNVYKK
                     HQKQTATKEAGIVEKSMPIHISNVVALNPKTKEPTSFRMKTLEDGTRVRVCKKTGETI
                     D"
     gene            5854..6405
                     /gene="rplE"
                     /locus_tag="PL056_06730"
     CDS             5854..6405
                     /gene="rplE"
                     /locus_tag="PL056_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013637822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HPM46745.1"
                     /translation="MSEKYISRLREQYEKEIKPALQKELGVGAMAVPKIEKIVVNMGV
                     GEAIANKKILEDAVKELTAIVGQKPLVNKAKKSIATFKLREGMPIGTSVTLRKDMMYD
                     FLDHLVNIALPRVKDFKGVSGKAFDGKGNYTIGIKEFFIFPEIDFNKVEKAKGFNITI
                     VTTAKNDKDAKLLLKKFNMPFNS"
     gene            6427..6612
                     /locus_tag="PL056_06735"
     CDS             6427..6612
                     /locus_tag="PL056_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009110294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HPM46746.1"
                     /translation="MAKKSIRIKAERKPKFGVRGYNRCPLCGRPRAFLRKFNMCRICF
                     RTLANQGLVPGVTKSSW"
     gene            6628..7026
                     /gene="rpsH"
                     /locus_tag="PL056_06740"
     CDS             6628..7026
                     /gene="rpsH"
                     /locus_tag="PL056_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HPM46747.1"
                     /translation="MAMNDLISNYLTRIRNGILAGKENVSGIPSSKIIEEISRVLKEE
                     GYISDFRINELNGKKTVSVDLKYYRNKSVISKIERVSTGGRRVYSQSAEIPFIREGLG
                     VSIITTSKGVMTDAKARELNVGGEIICKIW"
     gene            7039..7581
                     /gene="rplF"
                     /locus_tag="PL056_06745"
     CDS             7039..7581
                     /gene="rplF"
                     /locus_tag="PL056_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015955691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HPM46748.1"
                     /translation="MSRVGKSPIALSKDVKASLKDGVLTVQGPKATLERVIHPAVIVE
                     IDSEVINVRRIDESIETRSLHGLFRMLVSNMVTGVTKGFTKVLEVKGTGYKFEMKGDK
                     IGFSLGFSHPVEMKLPKGVSAEINKTNNELTLTCADKELLGDFAAKIKKLRPVEPYKG
                     KGIFYKGQVIRRKAGKAAGK"
     gene            7597..7956
                     /gene="rplR"
                     /locus_tag="PL056_06750"
     CDS             7597..7956
                     /gene="rplR"
                     /locus_tag="PL056_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HPM46749.1"
                     /translation="MRPDIKKERRIRRKAGVRKRISGTPERPRLSVFKSNVHIYAQVI
                     DDVAGVTLASASSDEKGFVKPEGDKKSVAVKVGQSIALKCKEKGIELVVFDRNGYPFH
                     GRLKALADAARESGLKF"
     gene            7966..8490
                     /gene="rpsE"
                     /locus_tag="PL056_06755"
     CDS             7966..8490
                     /gene="rpsE"
                     /locus_tag="PL056_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HPM46750.1"
                     /translation="MKEINKPINPEMMELNEKLVYVNRVAKVVKGGKNFSFAALMVVG
                     DGKGFVGIGLGKAREVPEAIRKGVVRAKKNLCQIKLLRGTVPHEITGEYGAGKVILRP
                     ASAGTGVIAGSAVRAVLEAAGVTDILSKSIGSNNPHNMAKATLNALLNVETIEEVEAK
                     RNVGVKDYQLKEKE"
     gene            8495..8944
                     /gene="rplO"
                     /locus_tag="PL056_06760"
     CDS             8495..8944
                     /gene="rplO"
                     /locus_tag="PL056_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HPM46751.1"
                     /translation="MQLYELKKPEGLKSRKRIGRGDGSGTGSTAGKGHKGQKARSGGG
                     VRRGFEGGTMPYNRRIPKRGFFNKWATDVCEINIRYLEKFESGSVVDLEKLKEAGLYN
                     GKDEVVKIIGRFDLTKAITVRVHKFTAGAKESIEKSGGKAEEISLDK"
     gene            8934..10250
                     /gene="secY"
                     /locus_tag="PL056_06765"
     CDS             8934..10250
                     /gene="secY"
                     /locus_tag="PL056_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015905421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HPM46752.1"
                     /translation="MISKVLKNIFKIPDLRERVIFTLLMLAVYRLGIFVTTPGVNRSV
                     MEKIMSTKATGTLLGLFNMFSGGAVENLSIFALGIMPYISASIVMSLLAVIVPKIGEL
                     QKEGDQGYRKITQYTRYGTVLLCVVQGFMMAKFLESLTGPVGEVVVNVPGWFFRIITV
                     ITLTAGTLFLMWLGEQITDRGIGNGVSLIIFASIVARFPNMFFQMGKYLKEQLIQPHQ
                     LVIFLIILVATFAIIVFVESSQRRIPVQYARRVAGGRKIQNSHLPLRINTSGVIPPIF
                     ASALLVFPATLSSFLKFDMGPLQGWLNPGGWLYNLLYLGLIVFFCFFYTFIQFKTEDV
                     AENINKQGGYIPGVRPGKETVTYINTVLSRITFGGAIYLSAVCMIPDLLRNKMGLPFY
                     MGGTSILIVVGVAMDMASQIESYLINNNYEGFSIGGKGSLVKSRRG"
     gene            10390..10620
                     /gene="infA"
                     /locus_tag="PL056_06770"
     CDS             10390..10620
                     /gene="infA"
                     /locus_tag="PL056_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HPM46753.1"
                     /translation="MVKEEGIVVEGTIVDTLPNAMFRVELENGHQVLAHISGKMRKYF
                     IRILPGDKVQIELSPYDLTRGRIVHRMKNNGN"
     gene            10634..10747
                     /gene="rpmJ"
                     /locus_tag="PL056_06775"
     CDS             10634..10747
                     /gene="rpmJ"
                     /locus_tag="PL056_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015851088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HPM46754.1"
                     /translation="MKVRASVKKICTKCKIVKRKGILRVICENPKHKQKQG"
BASE COUNT         3645 a         1840 c         2692 g         2615 t
ORIGIN      
        1 gggaaggcgg cagaactgta ggttcaggcg ttgtgtccaa gataattgaa tagatttttc
       61 aagaggtgtt gaagtgagcg acaagatcag aatcaaatta aaggcattcg atcacaagat
      121 acttgatcag tctgtaagcg agatcattga aactgtgtca cgcagcggag cgagaattgc
      181 aggtccgatc cccttaccga caaagatcca caaatattgt gtgcttcgtt ctcctcatgt
      241 agataaaaaa tcgcgtgagc agtttgagat gagaattcac agaaggattc ttgatattct
      301 caatccgacg aaccagacaa tggacgagct gatgaagaca gatttatctg ccggagttga
      361 aattgaaatc aagacttatt aaagggaatg ttaactttta tgtcataaag cagagagggt
      421 aactatggct gctggactca taggaagaaa attaggcatg actcagatct ttagggaaga
      481 cggcaaaatt ctgcctgtaa ccgtaattga agctggtccg tgctatgtaa ttcaaaaaaa
      541 agagaccgag aaggatggct atacagcgat tcaggtcggt tttgaaaaga aagagaaaaa
      601 tgtgaaaaaa ccggaagccg gtcattttaa aaatgccgca aaaggtgttt tcagaaaatt
      661 aaaggaattc agagttgccg ccaatgagat aggcagctat gaacttggct cggagcttac
      721 tgttacaatg ttcaagcccg gtgaaaagat caaggtaacc ggcttttcaa aaggcagggg
      781 ctttgcgggt gttgttaaga gatggagctt tggaggtgga agaacgacac acggttcgaa
      841 attccataga aaaggcggtt ccataggcca gaatgtctgc cctggaaaag tttggaaagg
      901 gaagaaaatg ccgggtcgtt acggtaacga acagatcacc gtaaaaaatc tggagatagt
      961 aagagttgtt ccggctgaca acacaatcgt tgtaaaaggt gcgatccccg gttccaccgg
     1021 cggtatcgtt tacattaaga agtagtggag gcgaacatgc ccgttgttga tttatttgac
     1081 agatcaaaga aaaaggtcgg tgaaatagag ctgaaagatg cagtttttgc tgtgccgttt
     1141 gaagaaaaga aagttctcat gtatgaagtc attaaaatgc agactgcctc ctggagaagc
     1201 ggaactcatt cgacaaaaac ctatgccacc atttcaggcg gtaattcgaa accctggaaa
     1261 cagaaaggaa cgggaagggc aagaagggga acgatgagag catcaacgct cagaggcgga
     1321 gctcctgctt ttggaccgca acccagagat tattcctaca caattcctaa aaagaaaaga
     1381 attgctgcaa tgagagctct tctttccgaa agactgagca aggggaacct ttttgtagtc
     1441 aaagcatttg atttcgatca gatcaagaca aaagaagctg tttccgtcct caaaggtaac
     1501 tgggatctca aggaagctgt tattgtcggc ggagacagtg aagaaaagtt tctcctgagc
     1561 gtcaggaaca ttcaggaata tcttttcctt cacaagactc agatcaatct ttacgacatc
     1621 atggcgtata agagtgtgat ttttacagag gaagcaatta aatacattga cgaggttcta
     1681 gggtaatgaa aaaggaaaaa catttcgaca ttatcaaaag accgcttttg actgaaaaat
     1741 ctgctgtgtt aagcaaagat cagaaacagt cttatgtttt cgaggtaaaa ctcgattcca
     1801 caaaagagga tgttaaagaa gcggtggaaa aagtttttaa cgttaaagtc gatagcgtta
     1861 acaccgttgt tgtgggcgga aaaacaaaaa gagtaggcag gacacttgga aagaggtcaa
     1921 cctggaaaaa agcctatgtg acattgaaag aaggccacca gatcaacctc gttgaaggac
     1981 tttaataatt gattaagtaa ggtttcggag aaaaattatg ggtatcaaga tttttaagcc
     2041 gacttctaac ggtcgcagaa atatgactgg aagcgactat tctgaaataa cgaagtcgaa
     2101 acccgagaaa tctctggtcg aaccgatcag aaaatccgga ggaaggaaca atacaggcag
     2161 aataacaatt cgccataggg gtggcggcaa caaaaccatg tacaggatca tagacttcaa
     2221 aaggaacaaa ttcgatgttc cgggaaaagt tgaagccatt gaatatgatc cgaacaggaa
     2281 cgcaagaata gcacttatat tctatcttga cggagaaaag agatatataa tcgctcccga
     2341 caagataaat gtaggagatg ttattgtttc cgccggtgaa aaagaagccg atatcaaacc
     2401 cggcaactca atgaaaataa agtttattcc cgtcggaaca ttcattcaca atgtggaact
     2461 caagataggt aaaggcggtc agatagcaag agcggcggct gcaggtgttc aggttctcgg
     2521 caaagagggt gatcatgcga cagtgagaat gcccagcggt gagataagaa tagttcacct
     2581 tgaatgcaga gcttctgtag gaaccgtcgg taaccaggag atatcaaaca tcaagatagg
     2641 taaggccggc aaacagagat ggctcggcaa gagaccccat gtccgcggtg ttgtaatgaa
     2701 cccggttgac catccgcacg gtggtggcga aggaaagaca tcaggtggtc gtcatcctgt
     2761 tactccttgg ggtaaaccga ctaaaggata ccgtacaagg aataataaga gaactgacaa
     2821 gtttagagtc agaagaagag acaagtaatt gtattaactg taaaaatatg gagggttgca
     2881 gtgcctcgtt cagttaaaaa agggcctttt gttgattcac atgtgtggaa gaaagttata
     2941 aaggcagttg acggcggaga taaaaaaccg atcaagacat ggtcaagaag atcaaccatt
     3001 atccccgaaa tggtcggtct cactttttcg gtatacaacg gacagaaatt catccctgtt
     3061 ttcgtaacag aagacatggt tggttttaaa cttggagagt tttccccgac gagaaccttc
     3121 aggtcacact caggcagcaa gaaataattc ttgcgtaatg ttaagctata tgtatgatat
     3181 agccgctaaa aatttttcga tcgttggaga tgcgatatgg aagacaaaat agtcagagca
     3241 cagctcagaa atttcaggat gtctgcaagc aaggtaagat tagttgcgga cattataaga
     3301 ggcaaaagcg ccggggacgc caagaacatt cttgagttca caccaagaaa agccgccatg
     3361 gctctgaaaa agcttcttga ttcggcaatt gctaacgctg aaaacaatta caatcttgat
     3421 cctgacacac tgtttctcaa aactgttatg gtcgatgaag gtcaggttat gaagcgtttc
     3481 gtaccgagag cacagggcag agctgccaaa atacttaaga gatcaagcca tgttacaatt
     3541 gagcttgcgg cgaataaata aggtttttac tatacggagg tgaactttgg gacagaaggt
     3601 taatcccatc gggcttagac tcggggtgac ccggacctgg gaatcggttt ggtacagtca
     3661 caagaattac agtaagtggc tgcatgagga tcttaaggta agaaaagcga tcaaaaagga
     3721 attctatcct gcaggtattg ccaagataga tatcgaaagg tcgggcgaaa agcttaaaat
     3781 aataattcat accgcaaaac ccggtattgt tgtcgggaga aaaggtgtcg gtattgaaaa
     3841 gatcaaggaa atggttcaga agatagctaa agatgtcact gatatctttg tgcagactga
     3901 agaggtaagg aagccggaaa tagatgcaca gctcgtaagt gaaaatgttg ctgaacagct
     3961 tgaaagaaga gctcatttca gaagagctat gaaaagagcg ataagccagg caatgaaaag
     4021 cggtgcggaa gggataaaga tccagacatc cggtcgtctc ggaggagccg aaatagcaag
     4081 aaccgagaga tatcaggagg gaagaactcc tttgcataca ctcagggcga acattgatta
     4141 cggatacgca attgcaaaaa caacttacgg tgttataggc gtaagagtgt ggatgtcaaa
     4201 gggcgactca atcgataaaa agcctgagaa taaaagaaaa cagaagaaaa attaaaatag
     4261 cgggtacgaa ctatgttaat gccgaataaa gtaaaacaca gaaaggtaat gaaagggaag
     4321 aggtccggtc tggcaacttc ctgtaacagc attgatttcg gagagttcgc tcttaaagct
     4381 accgaatgcg gatggatcaa cagcagacag atcgaagccg cccgtgtagc aataatgaga
     4441 gctgttaaaa gaggcggccg tatgtggata aggatattcc ccgataagcc tatcacaaaa
     4501 aagcctctgg aagtaagaat gggaaaggga aagggaaatc ctgaagaatg ggtttgcgtc
     4561 attaaaccgg gcagaatact ttttgaactt ggtgatgttg acagtgaaac tgctcttgag
     4621 gcattccgcc ttgcttctca taaacttcct atcagaacga aaatcataga aaaaggagat
     4681 gaactgaaat gaaagtttct gaattaagag aaaagacact ggctgaactc caggagaagc
     4741 ttctgtctta taaagaggag cagttcaact tgagaatgca gaaaggaatg gggcagatgg
     4801 aaaacaactc ccgtgctgta tccattaaaa aggacatagc gagaataaaa accatgataa
     4861 ccgagaaaaa gaaagcggtt gaggtgaaga aatgacagaa aaaataaaaa gaacacttac
     4921 gggtacagta gtttcagatg caatggataa aactgttgct gttcaggtta cgcgtcgttt
     4981 caaacatgcg ctgtacaaga aaatgatcac tgcccgcaaa aagtttcttg cccatgacga
     5041 gcagaatcag tacaaggtcg gagatattgt taaaatacag gaatgcgctc ctatgagcaa
     5101 aagaaaaacc tggtttgtaa aagaaaaaat tggtgagagc aggagatagc aatgatacag
     5161 gaaatgacaa gactggctgt tgccgacaat tcaggcgcca aggaagtcca gtgcattaag
     5221 gtttacggcg gttcaaaaat aagatatgcc ggtgtcggtg atgttatagt tgtcgctgta
     5281 aaagttgctg aaccgaaagg caaagtgaag aaaggcgatg taaagaaagc tgttattgtc
     5341 cgtcaggcaa aggagatccg tcgtgatgac ggttcctata taaggtttga tgacaatgca
     5401 gtcgtaataa tcgatgacgc atacgagcca atcggaacgc gcgttttcgg accagttgca
     5461 agagaacttc gtggcaaagg gttcatgaag atagtttctc tcgcaccgga agtattgtaa
     5521 ggggattacc atggctaaca ttaaaaagaa tgacaccgta aaggttattg ccggaaaaga
     5581 taaaggcaag agcggaaaag tattaaaggt tctcgttaaa gatgaaaagg ttgttgttga
     5641 aggtgttaat gtttacaaaa aacatcagaa acagaccgcg acaaaagaag caggcatagt
     5701 tgaaaaaagt atgccgatcc atatttcaaa tgttgtggct ttaaacccga agacaaaaga
     5761 gccgacatct ttcagaatga aaacacttga agatggaacc cgggtcaggg tatgcaaaaa
     5821 aaccggcgaa accattgact aacggagatg acaatgagtg aaaagtacat aagcagactt
     5881 agagaacagt atgaaaagga aataaagccc gcacttcaga aagagctggg tgtcggagca
     5941 atggctgttc ccaaaatcga gaaaatagtt gtcaatatgg gtgtcggtga agcgatagct
     6001 aacaagaaaa tacttgaaga tgctgtgaag gaactcacgg caatcgttgg acagaaacct
     6061 cttgttaaca aggcgaaaaa atcgatagcc acattcaagc tcagggaagg aatgccgata
     6121 ggaacatcag taactctgcg taaagacatg atgtatgatt ttcttgacca ccttgttaac
     6181 atagctcttc ccagagttaa ggatttcaaa ggtgtttcag gaaaggcatt cgatggaaaa
     6241 ggcaactata ctatcggtat aaaggaattt ttcatttttc cagagataga ttttaacaaa
     6301 gttgaaaaag caaaaggttt taacattact atcgttacga ctgcaaaaaa cgataaagat
     6361 gcaaagcttc tgcttaaaaa gttcaatatg cctttcaaca gttaattttt gagataggag
     6421 gataaattgg caaagaaaag cattcgcatc aaggcggaaa gaaagccgaa gttcggtgtc
     6481 agggggtaca acagatgtcc tctttgcgga agaccaaggg ctttccttag aaaatttaat
     6541 atgtgcagaa tatgtttcag aacgcttgct aaccagggtc ttgtacccgg cgttaccaag
     6601 tcaagctggt aaataatgag gtgaaatatg gcaatgaatg atttgatttc aaactatctt
     6661 accagaataa ggaacggtat ccttgctggc aaggagaatg tttcaggaat tccttcttcc
     6721 aagattatcg aggagatctc gagagtttta aaggaagagg gctacatcag tgatttcagg
     6781 atcaatgagc tcaatggaaa gaaaacagtt tcggtagatt tgaaatatta ccggaacaaa
     6841 agtgtaattt ccaagatcga gcgggtaagt accggtggaa gaagagtgta ttcacagtct
     6901 gccgagatcc cttttattcg tgaaggtctg ggtgtttcaa taattacaac atccaaaggc
     6961 gttatgaccg atgcaaaagc gagggaactt aatgtcggcg gagaaataat ctgtaaaata
     7021 tggtaatgga gggttaaagt gtcaagagta ggaaaatcac cgattgcatt gagcaaagat
     7081 gtaaaagcct cactcaagga cggtgtgctt acggtccagg gtccgaaggc aactcttgag
     7141 agagttatac atccggcagt aatcgttgaa atagattcag aagtaataaa tgtaagaaga
     7201 atcgatgaat ctattgaaac aaggtcactc cacggacttt tcaggatgct tgtaagcaac
     7261 atggtcacag gtgttacaaa gggatttaca aaggtacttg aagttaaagg caccgggtac
     7321 aaatttgaga tgaaagggga caaaatcgga ttttctttag gtttttctca tcctgtagag
     7381 atgaaattac ctaagggggt ttctgctgag atcaacaaaa ccaacaatga gttgaccctt
     7441 acatgtgcag acaaagagct ccttggagac tttgcggcaa agatcaaaaa actccgcccg
     7501 gtcgagcctt ataaagggaa aggtattttc tataagggtc aggtaattag aagaaaagcc
     7561 ggaaaggctg ctggtaaata gagcggaggt taaaaaatga gaccggatat aaaaaaagaa
     7621 agaagaatta gaagaaaagc tggtgtaaga aaaagaattt ccggcactcc ggaacgtcca
     7681 cggctttctg ttttcaagag caatgtacat atttatgctc aagtgatcga cgatgttgcg
     7741 ggagttacac ttgcttcagc atcatctgac gagaaaggtt ttgtaaaacc tgaaggtgac
     7801 aaaaaatctg ttgcagtaaa ggttggtcag tcgatcgcat taaaatgtaa ggaaaaagga
     7861 atagagctcg tcgttttcga caggaacgga tatcctttcc atggcagatt gaaggcactt
     7921 gccgatgcgg caagagaaag cggacttaaa ttttaggagt tgctgatgaa agaaataaat
     7981 aaaccgatca atccggaaat gatggagctt aacgaaaagc ttgtttatgt caacagggtt
     8041 gccaaggtcg taaaaggcgg taaaaacttc agttttgccg cattgatggt tgtgggtgac
     8101 ggcaaggggt ttgtcggcat agggcttgga aaagcaagag aagttccgga agctataagg
     8161 aagggagttg tcagggcaaa gaaaaacctt tgtcaaataa aacttctgag agggacagtt
     8221 cctcatgaaa taacaggtga atacggagcg ggcaaagtta ttctccgtcc tgcatcagca
     8281 ggtacgggag ttattgctgg atctgcagta agggcagtgc ttgaggcggc tggtgtaaca
     8341 gatattcttt caaagagtat cggttccaac aaccctcaca atatggctaa agcgacactt
     8401 aatgctcttt taaatgttga gaccattgag gaagttgaag caaagagaaa tgtcggagtt
     8461 aaagattatc agttgaagga aaaggagtaa caatatgcag ctgtacgaac ttaaaaagcc
     8521 cgaaggactc aagtccagaa aaagaatagg cagaggtgac ggaagcggta caggcagtac
     8581 ggcaggaaaa gggcataaag gacagaaagc ccgctccggc ggtggtgtga gaagaggatt
     8641 tgaaggcgga acaatgcctt ataacagaag aatcccgaaa agaggttttt tcaataagtg
     8701 ggcaacagat gtttgcgaga tcaacataag atatcttgag aaatttgaat cagggtctgt
     8761 tgtcgatctg gaaaaactta aagaagccgg tctttataac gggaaagacg aagttgttaa
     8821 gataattggc agatttgacc tgacaaaagc tataactgtc agagttcata agtttacggc
     8881 aggcgcaaaa gaatcgattg aaaaatcagg cggaaaagcc gaggagatta gtcttgataa
     8941 gtaaggtatt aaaaaacatt tttaagattc ctgacttgag agagagggtt atttttaccc
     9001 ttttgatgct cgcagtatat cgtcttggta tttttgttac cactccgggt gtcaaccgct
     9061 ctgtcatgga aaagataatg tcaacaaaag cgacgggcac gcttctgggt ttatttaata
     9121 tgttctcagg tggtgcagtg gaaaatcttt cgatctttgc tctgggcata atgccgtaca
     9181 tcagtgcatc gatcgtaatg tctcttctag ctgttatcgt tcccaaaata ggagagctcc
     9241 agaaggaagg tgaccagggt tacaggaaga taactcagta cacaagatac gggacagtgc
     9301 ttctgtgcgt agttcaggga tttatgatgg caaagtttct cgaaagtctc acaggaccgg
     9361 tcggggaagt agttgtaaat gtgcccggct ggtttttcag aataattact gtcataactc
     9421 tgacggcagg aacccttttc cttatgtggc ttggagagca gatcactgac agaggaatag
     9481 gcaacggcgt gtcactgata atttttgctt ctattgtagc aagatttcca aatatgtttt
     9541 ttcagatggg caagtactta aaagaacagc ttattcagcc gcatcagctt gtgatttttc
     9601 tcataatcct tgttgcaaca tttgccatca ttgtttttgt tgaaagctca cagagaagga
     9661 ttccggttca atatgcaaga agagttgcag gcggaaggaa gatacagaat tcacatcttc
     9721 cacttagaat aaacacatcg ggagtaattc ccccaatatt tgcaagcgca cttcttgttt
     9781 tcccggcaac tttgtcgagc ttccttaaat ttgacatggg tcctttgcag ggatggttga
     9841 acccgggtgg atggctctac aatcttcttt atctcggact tatagttttc ttctgttttt
     9901 tctatacttt tattcagttt aagacagagg atgtggctga aaacattaat aaacagggcg
     9961 gttacattcc tggtgtaaga ccgggaaagg aaacagtgac ctatataaac acggttcttt
    10021 caaggataac attcggcgga gctatatatc tttccgcagt atgcatgatc cccgatcttc
    10081 tcagaaacaa gatgggtctc cctttttata tgggcggaac atcaattctg atcgtagtcg
    10141 gtgttgcaat ggatatggca tcccagatag aaagctatct catcaacaac aactacgaag
    10201 gtttttcaat tggcgggaaa ggttcccttg ttaagagcag aagagggtga aagcaatgac
    10261 ttgctgtttt aaaatttgca ggaaaatgca taaaaaaatc ggcaagttat ccttatgtgc
    10321 cagaatttat ttgaaaaatc gtacaatacg attaaattca acatgttttt ttgatcagga
    10381 tgtgagataa tggtaaaaga agaagggatt gtagttgaag ggacaatagt tgacaccctt
    10441 ccaaatgcaa tgttcagagt tgagcttgaa aatgggcatc aggtgctcgc tcacatttcc
    10501 ggaaaaatga gaaaatattt tatccgtatt cttccggggg ataaggttca gatagaactt
    10561 tctccttatg atcttacaag aggaaggatt gtgcacagga tgaaaaataa cggtaattaa
    10621 ttaggagaga aagatgaaag ttcgtgcttc tgtaaaaaag atatgcacaa agtgcaagat
    10681 cgttaaaaga aaaggtattt taagggttat ttgtgaaaac ccgaagcata aacagaaaca
    10741 aggataagat agatcaggag gtgacagttg gttcgtatcg caggagttga tc
//
LOCUS       DAOOYB010000088        10790 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_6555_length_10790_cov_9.257569, whole genome shotgun sequence.
ACCESSION   DAOOYB010000088 DAOOYB010000000
VERSION     DAOOYB010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10790
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6555_length_10790_cov_9.257569"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            169..1047
                     /gene="pdxS"
                     /locus_tag="PL056_06780"
     CDS             169..1047
                     /gene="pdxS"
                     /locus_tag="PL056_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006785221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HPM46755.1"
                     /translation="MNDRYELNANLAQMLKGGVIMDVVNAEQAKIAQEAGAVAVMALE
                     RVPADIRKDGGVARMSDPALIIEIQKAVTIPVMAKARIGHFVEAQILEALEIDYIDES
                     EVLTMADEDNHINKHKFRIPFVCGARNLGEACRRISEGAAMIRTKGEAGTGDVVEAVR
                     HMRTMNKEIAELCSLNDEEVGTFCKNHGISYELAFRIREIKRLPVVNFAAGGIATPAD
                     AALMMQLGCDGVFVGSGIFKSGNPAIRAKAIVTATANFNDPKIIAEVSKNLGEPMVGI
                     NCSSIKEEDKYAKRGI"
     gene            1047..1670
                     /gene="pdxT"
                     /locus_tag="PL056_06785"
     CDS             1047..1670
                     /gene="pdxT"
                     /locus_tag="PL056_06785"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007063009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HPM46756.1"
                     /translation="MSRIAILDLQGGVAEHAAHIRDCGHEPVFVKKVEHLENVSGLIL
                     PGGESSTLRKLLKETGLDKEIIQKVKTGMKVWGTCAGAILAASKVVGEEPCLSIIDIE
                     IERNLFGSQLSSFTEFKVISKVSSQPVKLVFIRAPGIKSVWGDTKILLETKGVIVAAE
                     NESVLATSFHPELSPNREIHKYFIKKSGLQVKESTDIQWDQNRWMKR"
     gene            1703..2989
                     /locus_tag="PL056_06790"
     CDS             1703..2989
                     /locus_tag="PL056_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46757.1"
                     /translation="MKTIFRFVAPIVIVILIFSLLELFSWFYIKRFSVPTTQWDYRIQ
                     RPEPYKNAPFFSEEFVKEAFFQPGGWNVSEKTGDISPKDFSGRYFNTSDGIRKTSFQP
                     EKFENKVYIFGDSTVYGSEVPDEHTIPSQLQILFNKFYPEKYIVLNYGITSFSSDKQL
                     KMLKTVNDLKQGDIVIFYEGINEVFVNIFYANINSSYMKFVQNDLDALSWLLKIMVAL
                     SERSYFAKLFLNPVDYAVPEHLSDDVFVKKMTEQTRPGFTRDIIAAHSYSTKSGAKFF
                     HFLQPHFFADDTYTEYEEKIKNNKYLTPTGIEGSFKKGYEVLQKANAELAESMNSVDL
                     TEILNEHKDGEEYFLDYVHVNHSANKIIAENIFKNIRKGLSTLHKHHREELSLCGQSR
                     KVYHQIRLSNKRSLTPCTMFYVHSPLKTNYFTDIFL"
     gene            complement(2970..10733)
                     /locus_tag="PL056_06795"
     CDS             complement(2970..10733)
                     /locus_tag="PL056_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin-like protein"
                     /protein_id="HPM46758.1"
                     /translation="MFNLLKKLGSFVVLAIVLLSGCDNLSKESGTFSVKFTWPENNKP
                     DFSGDSYYAWIDIREWIDGDAENMKIHLKEGPVAFDNEGKLTLKLENLSYGKNRVIVV
                     EVRKSNSDQDRTLYYGISDMFDLDPGKHTDVTVDMNFTPGTVEGNNSFAIDIFQNDVK
                     ITSTANEKVSIRVFKATGSKVVFSNRLDLLDIQINNETRDYTGSEVIDFSTLTKIDDI
                     TFEYPEWDLTAGREDAENGDGNKTVYAKVIDSNGYRSEMAYASVNLDTTDPSVSNITI
                     SPAGDPGLANIGTQIVVKFIMSEPVTDLMLFSDGIEMIDKSSGSDMYFVYTVTDADVD
                     GSVYNFKVSAKDNAGNQLNDFPLGSVQIDSTLPELLNFSVSKNKVRTGEEFSVSLEVS
                     EALKNINVLVGAKSISDKCNLEDGSETKYICKHFADIDGDEGDGVKQIAVELTDLAGN
                     KMSQTLKKDAEPVTIEYDITAPILASAVILPETVNRSSDKINLKFSFAEKVNFDLSDL
                     VLDPDTDLVFDCGDISTASKSFECSAPFAKEDQRVADYNVSVSVSDEVGNESKDIELG
                     TISVDRISPFLSNQNVTPEAVKLNEEFVITFSVSEELLADPLVRVGDKTLPGSECGYS
                     STTSLWTCTHQANKDGDEPDGQKMVSVYIVDKAGNAATEILTKSINYDATPPVMINPV
                     FIPGAKLNSYDGLFWVRFSFSERVLNDDSFVFSYVTDEGDIFNKPVSCSSTDGMNQSF
                     SCAFNLLPEDNIQGKYTFFIDATDLSGNRLAQDGTSNKIGTIEVDRKLPEINIVSVDP
                     ENVNYETDHIEVVFTTNEPVQTLVNPEVKFGTNIQTSSPFSVNAERTEFIYHITNLQM
                     AYDGTNVINIKAGDLSGNTGDASRGGIHVDRTRPVVINSDLNKNLAREGEDIIVSVTF
                     SEEMDPSSVKIEDSGLGLIKDTHSSTDRIFVYLYTVKPSDAGIREKIYDISILGSDTF
                     DNKIAESYAIGTVILDLTPPDNSKITDLEIITATSSFYTVDSLPVASTYQPEIEYSFN
                     ITNASEIFERPEVFIGYNPAPITDCNGDETVISCYGTYNISGDEGEGVKHLTVNLYDA
                     VGNRVTYTPGAVIFDFTGPRLVSTSVTRDPAFAHSRDETGKIQYFSRKDPFSEKNVQL
                     VLTMYADELIDTDFSNTIDYLPCETDSYAVKPEEISGNSLTHIRTVNDCLGLYSPMVI
                     WRDFVGNETARESDWKISIVDDQPDPLNIKRDYITYYRKPFGSIDSTATPRFYVTSSP
                     KTVMSDDIVMVAFYTETGILAGTAVVGDDGSFSVDTLTGGDVPVIYINPVSFTGNKIP
                     VKDDLGNPYDIKTVLQPVPNVVWTANLNRKIQGSQWPNPTTFINTSAETNYLVRDSFL
                     NSYETSELPSSVYSGVSSSKRDGAQIQVFEDPMSSFYRTGRASAYNPAKDELLVFGGR
                     TVSDGSSMELWFNDLAVWNGDKWKIIETEGQTPDKSENASMVYFEAKDQYVLFGGSKN
                     IDMVQYYYSQTWIWTGNYWINTGKTGPKARDNGAMAYDPVRKKAVLFGGRDPYGTINP
                     IDGHKYADDGTWEWDGETWLNIPVSGPDPSAGEMVYDLTSRKLIYYDGISGSTWSYDG
                     LIWETLATGGQQLKNFNMVYDSLLNRTLIYGGIKPDYSYSNSVYSFNGSSWTALAAAP
                     YQIADHVMWYSKSKNTAYAAFGAYNDLWIDPFRNLLELKGSVWKERTFQTHGVASPSI
                     TYYPTKNKVYMFGGETDEFSDTYTFEFDGLRWDRVNNGATRPTTVTDNDLIYYPPADV
                     LLLFERSPARMWQYNGSTWSIRTTTGSPSKIDSYKMEYFPEQGHVVFIATTTEGNIET
                     WTYNGSTWTNVCRDGVPGGCIQPDLIFNEDFSISYNSQAQKVFGFMSGDHSGDIETWR
                     WTGSVWQAVSVTDPENDGTPGFIYNPSASYSQAYKSTIVSGTNNTDSFEIWLFNGTSY
                     RKIEQYDISKRFYFDSVWMENEQALLIRGGRVGRMSTDYFVKDYFVVKLPQNVVPTQQ
                     YIIPMSTAGMPNEAVIRKIDVSWIGGGTTKTETADNNLAILSFWYKNGWEELTYGSGS
                     SSSNSLMFEEINDPDIIDFMQNGIENNISFKINPKIIGTYSYSTPVISTSAVEVDVYY
                     NVEGKRDIPRYLDSQYYIPSKQFDYESARRTCLEWGGDLLIINNEDEENIVRDLVGFD
                     DSIQYWIGVNDIHRDLDFRTVSGRLIYSNGVIDGNYVKWAKYHPYTSTTDNCVYLLNG
                     RWSLGNCAVAGNFICEKTRENSYVISTKTENWANAKAVCELLNMDLVIMNGPFEQSKI
                     EKMTGGTTDFWIGYTDADVEGTWRWVNGLTGWIGNSEGTPYYYNNWGTGYPSTASGTY
                     DFAFISMAGNKKWRNNAAGTLLKHYICEENSEEMFCSLNNQDCTLSDECCSGICSDNY
                     GECVACLKNGDSSCAAGSNCCSGICNTSSKECAECLNLNQPTCTSATDCCDKSATCAS
                     GKCCYSLGLDCSLNSECCTGMSCGDESKCCKSLTTSCLLNSECCTGVCTGVTKKFCSC
                     RTTDQSCIINSNCCTGICSKGICQCSELGDQCLTTTDCCKGICSKGKCQ"
BASE COUNT         3091 a         2267 c         2102 g         3330 t
ORIGIN      
        1 aaattaaagt cacagcaaat atacaaagca ccaatatttt ttcaagttgt tacgcagagt
       61 tgcgattttt tatctgcacg gggttgacta agtcgatttg tgatattaca atgcagcgtt
      121 ttgtaagtta tttgaattat tgtgatataa cactttttgg aggtagaaat gaacgatcgt
      181 tatgaactta atgcaaacct tgcccagatg cttaaaggcg gagttatcat ggatgtagtg
      241 aatgccgagc aggcaaaaat agctcaagag gcaggtgcgg ttgcggttat ggcacttgaa
      301 agagttcccg cagacataag aaaggacggc ggtgttgcaa gaatgagtga tcctgcactt
      361 atcatcgaaa tccagaaagc tgtgacaatt cctgtaatgg caaaggcgag aataggtcac
      421 tttgttgaag cacagatact tgaagcactc gaaattgatt atattgatga aagtgaagtt
      481 cttacaatgg ctgatgaaga taaccacatc aataagcaca agttcagaat cccttttgtc
      541 tgcggagcca gaaatctcgg tgaagcatgc agaagaattt ctgaaggtgc ggcaatgata
      601 agaacaaaag gtgaagcagg aacaggtgat gtagttgagg ccgtcagaca catgagaacc
      661 atgaataaag agatcgctga actctgttct cttaatgatg aagaggtcgg aactttctgc
      721 aaaaaccacg gaatatctta tgaacttgca ttcagaataa gagagattaa aaggcttcct
      781 gtagtaaatt tcgcggctgg cggaatcgca actcctgctg atgcggctct catgatgcag
      841 cttggctgtg acggtgtttt tgtcggttca ggaattttta aatcaggaaa tcccgcaatc
      901 agggcaaaag cgatagtaac tgcgactgcc aatttcaatg atccgaaaat tattgctgaa
      961 gtttcaaaaa atctcggtga accgatggtc ggaatcaatt gctcaagtat caaagaagag
     1021 gataaatacg caaaaagagg gatctgatga gccgaattgc catacttgac cttcagggcg
     1081 gagttgcaga acatgccgcc cacataagag attgcggtca tgaaccggta ttcgtgaaaa
     1141 aagtagaaca tcttgaaaat gtttcagggc tcatacttcc cggaggagaa agctcaacac
     1201 ttagaaaact tctgaaagag accggtcttg acaaagaaat cattcaaaaa gtgaaaaccg
     1261 gaatgaaagt gtgggggact tgtgcaggag cgatacttgc cgcttcaaaa gtagtaggcg
     1321 aagagccttg tctgtcaata atcgacatcg aaattgaaag gaaccttttt ggttctcaac
     1381 tatccagttt tactgaattt aaagtcattt ccaaggtttc atcacaaccc gtaaaactcg
     1441 ttttcatcag agctcccgga ataaaaagtg tctgggggga caccaaaatc cttctggaaa
     1501 caaaaggggt gatcgttgcc gcggaaaatg aatcggttct tgcgacaagc tttcaccccg
     1561 aactctctcc aaacagggaa attcacaaat atttcattaa aaaatcaggt cttcaagtaa
     1621 aagaatcaac tgacatccaa tgggatcaaa accgctggat gaagagatag attaaagagt
     1681 gaaaatgtat aagccggatg ttatgaaaac aattttcaga ttcgttgctc caatcgtaat
     1741 agtgattctt attttttcac tccttgaact tttttcgtgg ttttatatta aaagattttc
     1801 tgttcctaca acacagtggg attatagaat acagcgtccc gaaccgtata aaaacgcacc
     1861 gttcttttct gaagagtttg ttaaagaagc tttttttcag ccgggtggat ggaatgtttc
     1921 agaaaaaaca ggggacattt ctccaaaaga tttttccggc aggtatttca acacttcaga
     1981 tgggatcaga aaaacctctt tccagccgga gaaatttgaa aataaggtgt atattttcgg
     2041 agattcgact gtttacggtt cagaagttcc cgatgaacat acgatcccaa gtcagctgca
     2101 gatacttttc aacaagttct accctgaaaa atatattgtc ctgaattacg gaataacttc
     2161 tttttcatca gacaaacaat taaagatgct taaaacggtc aacgacctga aacagggtga
     2221 cattgtgatc ttttatgaag ggataaatga ggtgtttgtt aatatttttt atgcaaatat
     2281 taattcatcc tatatgaaat ttgttcagaa cgatcttgat gcactttcat ggctgcttaa
     2341 gatcatggtc gctctttcag aacgatcata ttttgcaaaa ctattcctca atcctgttga
     2401 ttatgccgtt cctgagcatt tgtcagatga tgtttttgtg aagaagatga ctgaacagac
     2461 tcgacccgga ttcacaaggg atattattgc cgcacacagt tattccacta aatcaggagc
     2521 gaagttcttt cattttctgc agccccattt ctttgccgat gacacatata cggaatatga
     2581 ggaaaaaatt aaaaacaaca aatatctgac cccgacaggg atagagggct cttttaaaaa
     2641 agggtatgaa gtgctgcaaa aagccaatgc ggaacttgca gagtcaatga acagtgttga
     2701 tctgactgaa atacttaatg aacataaaga cggcgaagag tattttcttg attatgtcca
     2761 tgtcaatcac tcagccaata aaattatagc cgaaaatatc ttcaaaaaca tcagaaaggg
     2821 attaagcacg cttcataaac atcatcggga agaactttcc ctgtgcggtc aatctcgtaa
     2881 agtttatcat cagatccgtt tatcgaataa acgatcttta actccttgca ccatgttcta
     2941 tgttcattcc ccgctcaaaa caaattattt tactgacatt ttcctttaga gcaaatccct
     3001 ttacaacagt cagtcgtcgt taagcattga tctccaagtt cagaacactg acatattcct
     3061 ttactgcata ttcctgtgca gcagttggaa ttaataatac aagactggtc agttgtcctg
     3121 catgagcaga attttttagt aactccggta cagactcctg tacaacattc actattcagc
     3181 agacagcttg ttgttaagga tttgcagcat tttgattcat caccgcaact cattcctgtg
     3241 cagcattctg aatttaagga acagtcaagt ccgagcgaat aacagcattt ccctgaagca
     3301 catgtcgcag atttatcaca gcagtctgtt gcacttgtac aagtcggctg attaaggtta
     3361 aggcattccg cacattcttt tgacgatgtg ttacatattc cactgcagca gttgcttcct
     3421 gcagcacatg atgaatcgcc gttcttaagg catgccacac attcgccata gttatctgaa
     3481 cagattccgc tgcagcattc atcactcagc gtgcagtcct gattgttgag cgaacagaac
     3541 atctcttcac tgttttcttc gcagatatag tgtttcagca gtgttcctgc ggcattattt
     3601 ctccatttct tatttcctgc cattgaaatg aaagcaaagt cataagttcc ggatgccgtg
     3661 gacggatatc ctgttcccca gttgttgtaa taataaggcg ttccctctga attgcctatc
     3721 cagcccgtaa gtccgtttac ccagcgccag gttccttcaa catcggcatc ggtatagccg
     3781 atccagaaat cagttgttcc tccggtcatt ttttcaattt ttgactgctc aaaaggaccg
     3841 ttcattatca caaggtccat gttaagcaat tcacaaactg ctttggcatt tgcccagttt
     3901 tctgtttttg ttgaaatgac atagctgttc tcccttgttt tttcacaaat gaagtttcct
     3961 gcaacagcac agttcccaag agaccatctt ccgttcagaa ggtagacaca gttgtccgtg
     4021 gttgatgtgt aaggatggta tttcgcccat ttcacataat taccatcaat aactccattg
     4081 ctatatatca gtcttccgct cacagttctg aaatcaagat ccctgtgaat gtcgttaact
     4141 cctatccagt attgtatcga atcatcaaat ccgacaagat ccctgactat attctcttcg
     4201 tcttcattgt taataattag aagatctccg ccccattcaa ggcatgtccg tcttgctgat
     4261 tcataatcga attgttttga aggtatgtaa tactgactgt caagatagcg gggaatgtcc
     4321 cgtttccctt caacattgta gtacacatcc acttcaactg ccgaagttga aatgacagga
     4381 gtggagtatg aataggtccc gatgatcttc ggattgattt tgaaagaaat gttgttctct
     4441 ataccatttt gcatgaaatc aataatgtca ggatcattaa tctcttcaaa cattaaactg
     4501 ttggaacttg aacttcctga tccgtatgtc aattcctccc atccgttttt ataccagaag
     4561 cttaatattg ccaagttgtt atccgctgtt tctgtttttg ttgttccacc gcctatccat
     4621 gaaacatcaa ttttacggat aactgcttca ttcggcatac cggctgtact catcggaata
     4681 atgtactgtt gagtcggaac aacattttga ggcagtttaa caacaaaata gtctttcaca
     4741 aagtaatctg tcgacatcct gccgacccga ccgcctctga taagaagggc ctgttcgttt
     4801 tccatccaca cagagtcaaa atagaacctt tttgaaatat catactgttc tattttcctg
     4861 taactggttc cgttaaaaag ccatatctca aatgaatctg tattgttcgt tccggaaaca
     4921 attgtgcttt tgtaggcctg agaataactt gccgacggat tgtaaataaa tccgggagtc
     4981 ccgtcatttt caggatcggt tacagatact gcctgccata ctgatccggt ccatctccat
     5041 gtttcaatgt ctccgctgtg atctccggac ataaatccga acactttttg agcttgcgaa
     5101 ttatatgata tcgagaaatc ctcattaaat atcagatccg gctggatgca tcctcccgga
     5161 acaccgtctc tgcagacatt tgtccatgtt gatccgttgt aagtccatgt ctcaatattt
     5221 ccttctgttg tagtggcaat aaatacgaca tgcccctgtt ccggaaagta ttccattttg
     5281 taactgtcaa tttttgaagg acttcccgtt gtggtcctga tagaccatgt ggagccgtta
     5341 tattgccaca ttctcgccgg tgatctttcg aaaagaagaa gaacatcggc aggcgggtag
     5401 taaatcagat cattatccgt gacagttgtc ggcctggttg ctccgttatt tacacgatcc
     5461 catctcaatc catcaaattc aaaagtgtat gtgtctgaga attcatcagt ttctcctccg
     5521 aacatataca ctttattttt ggtaggataa taagttattg atggagatgc aacgccatga
     5581 gtctggaatg tccgctcttt ccatactgac cctttaagtt caagaagatt tctgaagggg
     5641 tctatccata ggtcattata ggcgccgaaa gcggcatatg cggtgttctt tgattttgaa
     5701 taccacatga catggtcagc aatctgatat ggtgccgcag caagagcagt ccatgatgat
     5761 ccgttaaatg aataaactga attactgtaa ctgtaatcag gttttattcc gccgtagata
     5821 agagttctgt tcagaagcga atcatacacc atattgaaat ttttaagttg ctgccctccg
     5881 gttgcaagag tttcccaaat taaaccgtca taactccatg ttgatccgga tatgccgtca
     5941 tagtatatca attttcttga agtcagatca taaaccattt cccccgcaga aggatcaggc
     6001 cctgaaaccg gaatattcaa ccaggtttct ccatcccatt cccatgttcc gtcatctgca
     6061 tatttatgtc cgtcaatcgg atttatagtt ccgtacgggt ctcttccacc gaaaagaaca
     6121 gcttttttcc ttaccggatc ataggccatt gcaccattgt ctctggcttt agggccggtt
     6181 tttcctgtat tgatccagta gtttcctgtc catatccatg tctgtgaata ataatactga
     6241 accatatcaa tatttttaga tccgccgaaa agaacatact gatcttttgc ttcgaaatag
     6301 accattgatg cattttcaga tttgtcgggg gtctgacctt cagtttcgat tatcttccac
     6361 ttatccccgt tccatactgc aagatcatta aaccacagtt ccattgaact tccatcagaa
     6421 acagtccttc ctccgaaaac aagaagttcg tctttggcgg gattatatgc agaagctcta
     6481 cctgtccggt aaaaagaact cataggatct tcaaacacct gaatttgagc accatctctt
     6541 tttgaagatg aaactcctga ataaacagaa gaaggaagct ctgaagtctc ataagaatta
     6601 agaaagctgt ctcttacaag atagttagtt tctgcagaag tgtttataaa tgttgtcgga
     6661 ttcggccact ggcttccctg tatctttctg ttcagatttg cagtccacac aacattggga
     6721 accggctgaa gaacggtttt tatatcgtaa ggattcccga gatcatcttt aacaggtatt
     6781 ttgtttcctg taaatgaaac aggattgata taaataaccg gaacatctcc tccggtaagg
     6841 gtgtcaacgc tgaaagaacc gtcatctccg acaacggcag ttccggcaag aatccctgtt
     6901 tcagtataga aagcaaccat tacaatgtca tcagacataa cagttttggg tgaagatgtt
     6961 acataaaatc taggtgttgc ggtagaatcg atcgatccga aaggttttct ataataagtt
     7021 atgtaatctc tttttatatt gagaggatca ggctgatcgt ctacaatgga tattttccag
     7081 tctgattccc gagctgtttc gttgccgaca aaatctctcc atattaccat tggagagtat
     7141 agacctaaac aatcattgac agttctaatg tgagtcaaag aattgccgct tatctcctca
     7201 ggcttgacgg cataactgtc ggtttcgcat ggaagatagt caatcgtgtt tgaaaaatct
     7261 gtatctatta attcatcggc atacattgta agaacaagct gaacattctt ttcgctaaac
     7321 gggtcttttc tgctgaaata ctgaattttt ccagtttcat cccttgaatg tgcaaatgcc
     7381 ggatctcttg ttaccgaagt gcttacaagc cgcggtcctg tgaaatcaaa aatgacagct
     7441 cccggagtat atgtaactct gtttcctaca gcgtcataaa ggtttaccgt caaatgtttg
     7501 acaccctcac cttcatcgcc tgatatattg tatgttccat agcagctaat tactgtttcg
     7561 tcgccattgc agtctgttat gggtgcggga ttatagccga taaaaacctc aggtcgttca
     7621 aaaatttcag atgcatttgt aatattgaat gaatattcta tttccggctg atatgttgat
     7681 gcaacaggaa gagaatcaac agtataaaat gaacttgtag ctgtaattat ctccagatca
     7741 gtaatttttg aattatcggg aggggtaaga tcgagtataa ctgttccgat tgcataagat
     7801 tcagcaattt tattatcgaa tgtgtcgctc cccaagatcg aaatatcata gatcttttct
     7861 cttattcctg catcagacgg tttaacagtg taaagatata cgaatattct gtcagtggaa
     7921 ctgtgtgtat cttttatcag accgagtccg ctgtcttcta tttttactga tgagggatcc
     7981 atttcttcag aaaatgttac cgaaactata atatcctctc cttctctggc taaatttttg
     8041 ttgagatcgg agtttattac tacaggtctt gtcctgtcaa catggattcc gcctcttgag
     8101 gcatcaccgg tgttgccgga aagatccccg gctttgatat ttattacatt tgtgccatca
     8161 tacgccatct gcagatttgt tatgtgatat atgaactcag ttctttccgc attaacggaa
     8221 aagggtgaag aggtctgaat attggtcccg aacttaacct cgggatttac aagtgtctga
     8281 accggctcat ttgtagtaaa cactacctca atatgatccg tttcataatt tacattttcg
     8341 ggatcgacag atacaatgtt tatctcagga agttttctat caacttcgat cgtgcctatt
     8401 ttgtttgatg tgccgtcctg agcaagccgg ttgccgctaa ggtctgtcgc atcaatgaaa
     8461 aatgtgtatt taccctgaat attgtcttca ggcagaaggt tgaatgcaca tgaaaatgac
     8521 tggttcatac cgtcagttga agaacatgaa accggcttat tgaatatgtc cccttcatct
     8581 gtgacataag aaaaaacaaa gctgtcgtca ttcagaaccc tttctgaaaa actgaatctg
     8641 acccagaaaa gtccgtcata gctgttaagt ttagctcccg gtatgaaaac cgggtttatc
     8701 ataacaggcg gagtcgcatc ataattaatt gattttgtta atatttcagt tgcggcattc
     8761 cctgctttat caacaatata gacagagacc attttctgtc catccggctc atctccatcc
     8821 ttatttgcct gatgagtaca tgtccaaaga gatgttgtgg aactatagcc gcattcgctt
     8881 cccggcagag tcttgtctcc taccctcaca agaggatcag caagaagctc ttcactgaca
     8941 ctgaaggtta ttacaaattc ctcattaagt tttactgctt caggtgtaac attctgattt
     9001 gaaagaaaag gagatatcct gtcaaccgat attgttccaa gctctatatc tttgctttca
     9061 ttacccactt catcactcac agaaactgaa acattgtaat ctgcaactct ttggtcttct
     9121 ttggcaaaag gtgcagaaca ttcaaagctt tttgaagcag ttgaaatatc tccgcaatca
     9181 aaaaccaggt cggtatcagg gtcaagaaca agatcactta gatcaaagtt tactttttca
     9241 gcaaatgaga atttaagatt tatcttgtca gacgatctat tcacagtttc cggaagtatc
     9301 acagcactcg caagtatcgg tgctgtaatg tcatattcaa ttgttacagg ttcagcatct
     9361 ttttttaatg tctgagacat tttgttccct gcaagatctg taagttcaac agcaatctgc
     9421 ttaactccgt caccttcatc tccatcaatg tcggcaaaat gtttgcagat atattttgtt
     9481 tcagatccgt cctcaagatt gcatttatct gatattgatt ttgctccgac gagaacattg
     9541 atattcttca atgcctctga aacttcaaga gatactgaga attcttctcc cgtccggacc
     9601 ttgttttttg aaactgagaa attcaacaac tcaggcaatg tactgtctat ctgaacgctt
     9661 ccgagaggaa agtcatttaa ttggtttcct gcattgtctt ttgctgaaac tttaaaatta
     9721 taaacggaac cgtcaacatc agcatctgtg acagtataga cgaagtacat gtcagatcca
     9781 cttgatttgt caatcatttc aatcccgtct gaaaacagca tcagatctgt taccggctcg
     9841 ctcattatga actttacaac gatctgcgtc ccaatgtttg ccagaccggg atctccggca
     9901 ggggaaatag taatgtttga tactgacggg tcggtcgtgt caagatttac tgatgcatat
     9961 gccatttcac ttcgataacc gttagagtct atgacttttg catagacggt cttgttgcca
    10021 tccccgtttt cagcatcctc tcttcctgcg gtgaggtccc attccgggta ttcaaatgtt
    10081 atgtcatcta tttttgtaag tgtcgaaaaa tcaataactt ctgaaccggt ataatctctt
    10141 gtttcgttat ttatctgaat gtcgagcaga tcaagtctgt ttgaaaatac cacttttgaa
    10201 ccggtggctt taaaaacccg tattgaaact ttttcattcg cggtacttgt tattttgaca
    10261 tcattctgaa atatatctat ggcgaaactg ttgttgcctt ctacagttcc cggagtgaag
    10321 ttcatgtcca ctgtaacatc ggtgtgtttt ccgggatcaa gatcaaacat gtcagatatt
    10381 ccgtaataaa gagttctgtc ctgatcagaa ttcgattttc tcacctctac cacgatgacc
    10441 ctgttctttc cgtatgaaag gttttcaagt ttgagggtca gtttcccttc attgtcaaag
    10501 gcgacaggac cttctttaag gtggatcttc atgttttcag catcaccgtc tatccactct
    10561 ctgatatcta tccatgcata gtatgaatca ccggaaaaat cgggcttgtt gttttcaggc
    10621 catgtgaact tgaccgaaaa tgtcccgctt tccttgctca gattgtcaca accggaaagc
    10681 aggacaattg ctaaaacaac aaaagaacca agttttttta acaggttaaa cattttgacc
    10741 tcttatatta aaagtatact gcatcctttg tttttctttt ctgattcttc
//
LOCUS       DAOOYB010000089        10726 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_6618_length_10726_cov_12.340830, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000089 DAOOYB010000000
VERSION     DAOOYB010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10726
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6618_length_10726_cov_12.340830"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            75..743
                     /locus_tag="PL056_06800"
     CDS             75..743
                     /locus_tag="PL056_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46759.1"
                     /translation="MNIKFVFMILPVLFMLSCIQETTDKYSNPSYLTDSESYYKDYPD
                     ETGVSDDDPENEVWDVRFSFLGVINDGNAAAENIQYGSGNLTYLDLENNSISINSAMW
                     AIKKSIKISSGHEVPIIQIFFADDPANTSEEGKVTYYILQLEGSSIAKSETYYLNNDK
                     IYRAEVTLEEELIKEICYVQEPYSNKGRVTIFTHNIRVGEELRVEGVANMFNMEIIEC
                     KVLN"
     gene            725..1840
                     /gene="add"
                     /locus_tag="PL056_06805"
     CDS             725..1840
                     /gene="add"
                     /locus_tag="PL056_06805"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HPM46760.1"
                     /translation="MQSSELSKKGDSGLKCTEKLTSEFFRILPKTDLHVHLDGSLRLE
                     TILELAKKEKIELGASNVEELKALIGPGKTHKSLNDYLKGFDITLKVLQTREGLYRAA
                     YELCEDCASENIEYFEVRYSPVLHTNLGLPLPAIVESVVEGLKAGQRDFKVNSGVIIC
                     GIRNISPEVSLRMAELAVAFKNRGVVGFDLAGAEYNYPARDHLEAFSLILSNNVNVTI
                     HAGEAYGPESIHQALHYCGAHRIGHGVRLREDGDLLNYVNDHRIPLELCPSSNVQTGA
                     VRNIKSHPLKFYLDLGIRATINTDNRLITDTTMSKEYELIYNTFNLTCQNILDLVING
                     IKSSFMPFEQKKWYLRDTKSKVVGLIKEHTGMNIRNP"
     gene            1842..2405
                     /locus_tag="PL056_06810"
     CDS             1842..2405
                     /locus_tag="PL056_06810"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPM46761.1"
                     /translation="MTASKERKLIENAALGDVRSFEELLESVEDKVFSFALSISGGNR
                     SAAEDIYQEAVIKAYLNIGKFSFKSSFSTWLWKIIRNAYFDHLKFERKCGDLSIEDLE
                     GFEPSIDEKQELSLISDDRAVMLRKLISFLPVSYSEVITLVDLQEMDHDEAAQLLEID
                     KNLLKVRLHRARARLEQLIENNMEYFK"
     gene            2473..3063
                     /locus_tag="PL056_06815"
     CDS             2473..3063
                     /locus_tag="PL056_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46762.1"
                     /translation="MTERSEKILSELIAAGKKEKITVPLHIKHSLHNKLNAVAVKNNN
                     KFSKFIKPLLAVAAVAAVVCSVSFAVFNNSSEKIERYLIHESTVAGGRPVTIKLVYNA
                     ADDLRNVKFSLDLDEGISFHSDNPEIKSRKNHEWTGELKKGVNSIPFVVKTDRFGKMK
                     IVAKAVSGKFRHTQEIVLEARESDTVVSMFVVLTDN"
     gene            3064..3966
                     /locus_tag="PL056_06820"
     CDS             3064..3966
                     /locus_tag="PL056_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46763.1"
                     /translation="MTLKKCLLKIVFISILAVSPFVFSESNDPFTGAVEVEDGENVVN
                     WEEGIFGPQQKKKKEEEVDNDRKEEQKKEIVKKEEKPVKNDVTADDRQDAENVPENTV
                     ENSEPDENIEEYGKNSPEWGKYYGENIKVTEEGKFVEVQQKDPVQKEDAALAQNEEPF
                     MFPIDLLLWSHGGDGYNAMINTGVKVSYWNVYSVLSVGTDFSTIAARPLSLGVTAGGF
                     YRISDFSITADLGYEKVWDFGDNAAINDYALGLRAGLSYSVLSWFAISAGAGVNYSIF
                     KDSSFEEGKFIPMFFGGFEFSLIK"
     gene            complement(4111..5904)
                     /locus_tag="PL056_06825"
     CDS             complement(4111..5904)
                     /locus_tag="PL056_06825"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPM46764.1"
                     /translation="MKIDLISIHIKKSPQAMALGTAMIKAAIDSTSELKDLLNVSCFD
                     YYLDQHIDIIADSILIRKPDAVGFSTYLWNTAHVMKIAGIIKDRSPETYLFAGGAEPS
                     SSPEKLINSGLFDFVIKGEGELATVEAIGRIFNNKPLEQSIFSVPVADINELPSPFLN
                     GTVKLENNGGVLWELSRGCPFKCDFCFESRGVSNVRTFSLERIKAELELFEKHNISQV
                     FVLDPTFNKNRERAKKILKMIAQTAPEIHFTFEVRTEFLDREMAKLFKSVNCGLQIGL
                     QSSDPAVLSNVNRFINPSNFKEKIKILNSENIVFGLDLIYGLPGDTLAGFKKSIDFAV
                     SLQPNNLDIFPLAIFPGTVIHEKADSFGINFSQSAPYTVISTPSFSKEDIEAAAVITD
                     GCNVFYNQGKSVGWLFMILETLKISGSDFFEEFASFLSYRDRLDIEKLQLDFVKKLFE
                     SHNLITLYKPMENIIRLNHSLDRSLEAGFATLKEKSTKPPQNQILAASKGTFLITLDY
                     DPDDLLNIGHMTLKDFVKHFRPQKTEVVTYNSNGESIFTALDPKWARLLRKFNGENTL
                     SRVIEALKLQYKGEVAEFIEFCISSGILESS"
     gene            6151..7536
                     /locus_tag="PL056_06830"
     CDS             6151..7536
                     /locus_tag="PL056_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016199036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HPM46765.1"
                     /translation="MNLPYAEPFRIKVVEPLKTSTKEERVKWIKDARFNLFNLRSEQV
                     FIDLLTDSGTGSMSSHQWAEMMLGDESYAGASSFYKLKETIGELLGFPYFLPTHQGRA
                     AENVLFSAMIGKDGTGKVVPGNSHFDTTKGHIEFRNARAIDCTIDEAFDTTVDHPFKG
                     NINLEKLEKVFIQYGKDNIPLCIITVTCNSSGGQPVSMQNIKDVSALCRKYGITVIFD
                     AARFAENAYFIKIREDGYQNKTIKEIVYEMFSFVDGCTMSSKKDGVVNIGGFIGLRSR
                     ELFEKASVFNIVYEGYITYGGMAGRDMGALSVGLKESTEFDFLRQRIGQIEYLGKLLK
                     DYGVPVQEPFGGHAIFVDAKRFLSHIPKEQFIAQTLGVELYIEGGVRGVEIGTLLADR
                     DPDTRENRYPNLELLRLAVGRRLYTDNHMAYVAAALKNVYDRRNEIKTGLKIIKEAPI
                     MRHFTVELERA"
     gene            7555..10068
                     /locus_tag="PL056_06835"
     CDS             7555..10068
                     /locus_tag="PL056_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015775245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HPM46766.1"
                     /translation="MSEFKKLVDSPAGSRFIVQGNTAFALGVVHAGYHAADGYPGTPS
                     TEVIDKNLKYVADRMTVGWSVNEAVATAVTIGHSVAGMDALVTMKIPGLFQAGDTITT
                     SAFYTGQSGAFVVYAATDYVPSSTQHVIDARYFLSAARIPVLEPRDHQEMYDIAFLAA
                     DISRKFRTQVCVLASGILAHSEGLVTTREPRKIEPRPLPQNLHEWMLMPGIARKNYNE
                     VNKVRIPQLLEYAEKSEDFMSEHIGTDDWGVIVVGESDIIVKEAFVTIKADPSILSMS
                     IANPVPVNRIREFAAKVTGPLFVFEDGDVFLQEKIAVEGIKVIGKEKNSVITDWTPFE
                     VISFLKKHIQISFDITPLKIDVKPLMRPPSICPGCPYKAYGLAAENLRKKGKLYAGFG
                     EIGCSTLLYFNKGIDTILCMGGSDAMRQGFCMSRPEMVSKVVSVIGDSCECHSGLDAT
                     RNGVFRNVPGVQVVLDNRITAMTGGQPAPTSEMNLAGVPNKFNLKKAIAAEECNVVAV
                     NAYDLKGVEKALTEALDEAERGQFTNLVLEGACQQIVDPKLKKRTIRFDREKCKKCGR
                     CNICPGIKFGEDGYPEFTNLCINCAGNTQVCMQRCPFGAIVPIDEESRKVSEKIELPA
                     PKVFTSATVKKEDLPESLRIAIRGIGGQGNLFFGKVLSEVALRTPYSEARIVKGDTHG
                     MAQLGGSVISTFACGNAYSPILSPGSTDILVVMETSEVLREGFLELLKPGGTIVLNRF
                     EAVPPTAKKEDYPKFDDIKKALSNYKVIEFDALETVSKLGDKLGRTANVAVLGLLSTI
                     EPLNKIPLEIWMSALMKLSPKDSIRSANQIAFEAGRSAK"
     gene            10065..10544
                     /locus_tag="PL056_06840"
     CDS             10065..10544
                     /locus_tag="PL056_06840"
                     /inference="COORDINATES: protein motif:HMM:NF024135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HPM46767.1"
                     /translation="MSKVLIGYFSKTGSTKEIAERIGANLAKKGVESDVLELEKIKNI
                     ENYSSVILGAPINGMKHVPEFIKFIEENKTALDGKVKGLFTVAYIYYDGRSLWRSFIN
                     KSITKIEKLINPKNSIVFAGRIDKKMPAFANLLFGIGSDLPNDLINHEEIDKWSEGV"
BASE COUNT         3326 a         2002 c         2443 g         2955 t
ORIGIN      
        1 caaacaaaat attgataggt ttcaagtttg tggtataatg ctctgtttta tgttcgggac
       61 atttggagtc agcgatgaat attaaatttg ttttcatgat cttaccggtg cttttcatgt
      121 tgtcatgcat tcaggagacg acagataagt acagcaatcc gagctatctg accgacagcg
      181 aatcttatta caaggattat cctgatgaaa caggggtcag tgacgatgat cctgaaaacg
      241 aagtgtggga tgtgcggttc agttttctgg gcgttataaa tgacggaaat gcggcggcgg
      301 aaaatattca gtacggatca ggcaatctga catatcttga ccttgaaaac aacagtatct
      361 caataaattc agcgatgtgg gcgataaaaa aatcgatcaa gatcagttca ggacacgaag
      421 ttccgataat ccagatattt tttgccgatg atccggcaaa taccagcgaa gaggggaagg
      481 tcacatatta cattctccag cttgagggtt catcgatcgc aaaatctgaa acctattatc
      541 tcaacaacga caagatatac cgtgcagaag tgactcttga agaagaattg ataaaagaga
      601 tctgctatgt tcaggagccc tattccaaca agggaagagt cacaatattc acccacaata
      661 taagagtcgg tgaagaactc agggtggaag gtgtcgccaa tatgttcaac atggagatca
      721 tcgagtgcaa agttctgaac taagcaaaaa aggggattca ggactcaaat gcacagaaaa
      781 gcttacctct gaatttttcc ggatacttcc taaaactgat cttcatgtcc atcttgacgg
      841 atcgctccgt cttgaaacga tactcgagct tgcaaaaaag gaaaagatag agctcggggc
      901 ttcaaatgtt gaagaactga aagcgctcat cggtcctggc aaaacccata aatcactgaa
      961 tgattatctc aaagggtttg atattacttt gaaagttctc cagacacggg aagggcttta
     1021 cagggcggct tatgaacttt gtgaagactg cgctagtgag aacatcgaat attttgaagt
     1081 tcgttactcg cctgtgcttc acactaacct gggccttcct cttcctgcaa ttgttgaatc
     1141 tgttgtcgaa gggctgaagg cgggacagcg tgatttcaag gtcaacagcg gagtcatcat
     1201 ctgcggaata aggaatatct ctcctgaagt gagtctcaga atggccgaac ttgctgtagc
     1261 tttcaagaac agaggtgttg tcggatttga tcttgcaggt gctgaataca actatccggc
     1321 aagagatcat cttgaagcat tttcactcat cctttcaaac aatgtcaatg tgacaataca
     1381 cgccggagag gcatacggac ccgagagcat tcatcaggca cttcattact gcggggcaca
     1441 caggatcggt cacggtgtcc ggctcagaga ggacggcgat ctgctcaact atgtcaatga
     1501 ccacaggata cctcttgaac tgtgcccttc aagcaatgtc cagaccggag cggtaaggaa
     1561 cataaaatcg catcctctca aattttatct cgatcttggt atcagggcta cgataaatac
     1621 cgacaaccgg ctgataaccg acacaacaat gtcaaaggaa tatgagctga tctacaacac
     1681 tttcaacctt acatgccaga acattcttga ccttgtcata aacgggataa aaagctcttt
     1741 catgcctttt gagcagaaaa agtggtatct ccgtgataca aagagcaagg tcgtcgggct
     1801 cataaaagag catacaggaa tgaacatcag aaatccttaa aatgacagct tcaaaagaaa
     1861 gaaaattaat agaaaatgcg gccctgggtg atgtcaggtc atttgaggaa ctacttgaaa
     1921 gtgttgaaga taaagtgttt tcatttgctc tttcaataag cggagggaac agaagcgctg
     1981 ccgaggacat ttatcaggaa gcggtaatta aagcatatct gaacattgga aaattctctt
     2041 tcaagtcctc tttttcaaca tggctttgga aaataatcag gaatgcctat ttcgatcatc
     2101 tcaaatttga aagaaaatgc ggagacctga gtattgaaga tcttgaaggt tttgagccgt
     2161 caattgatga aaaacaggag ctctctctaa tatctgatga cagggcggta atgcttagaa
     2221 aacttatttc gttccttcct gtatcatatt ctgaagttat tacgctcgtc gatcttcagg
     2281 aaatggatca tgatgaagct gcgcaactcc ttgaaattga taagaatctt cttaaagtcc
     2341 ggcttcacag ggcacgagcc cggcttgaac agttgataga aaataacatg gaatacttta
     2401 aatgatgtgt aacttccaac aagataacgg tgtcttaatt atcggagtgg ttgggaataa
     2461 ggagttcaaa ctgtgactga aagatctgaa aaaatactaa gtgaacttat tgcggccggg
     2521 aaaaaggaaa aaataacagt tccacttcat ataaagcatt cccttcacaa taaattaaat
     2581 gcagtggcag ttaaaaacaa caataaattt agcaagttca taaagccctt gcttgccgtg
     2641 gcggctgttg ctgcagttgt ctgttcggtc tcatttgcag ttttcaataa cagttcagaa
     2701 aagatcgaaa gataccttat acacgagtca actgttgccg gtggcagacc tgtgacgata
     2761 aaacttgtct acaatgccgc tgatgatctt agaaatgtta aattttcact tgatcttgac
     2821 gaagggattt cgttccattc tgacaatcct gaaataaaga gcagaaaaaa ccatgaatgg
     2881 acaggtgagc tcaaaaaagg agtaaattcg ataccttttg ttgtaaagac agaccgtttc
     2941 gggaaaatga agatcgttgc aaaagcggtt tcaggaaagt tccggcatac ccaggaaata
     3001 gttcttgaag ctcgcgaatc agatactgtt gtttcaatgt ttgttgtctt gacggataac
     3061 tagatgacct taaaaaaatg tcttttaaaa attgttttta tatcaattct tgcagtttct
     3121 ccattcgttt tttctgaatc aaatgatccc tttacgggtg cggttgaagt tgaagacggt
     3181 gaaaatgttg tgaactggga ggaagggata ttcggccctc aacagaaaaa gaaaaaagaa
     3241 gaagaagttg ataatgacag aaaagaggag cagaagaaag aaatagtaaa gaaagaggaa
     3301 aaaccggtaa aaaatgatgt aacggctgat gaccgtcagg atgctgaaaa tgtgccagaa
     3361 aacacagttg aaaattcaga acctgatgaa aatattgaag aatatggaaa gaacagtcct
     3421 gagtggggaa aatattacgg ggaaaacatc aaagtaaccg aagaaggaaa atttgttgaa
     3481 gttcagcaaa aagatcctgt tcaaaaagaa gatgctgcgc ttgctcaaaa cgaagaaccg
     3541 ttcatgttcc cgatagatct tcttttatgg agtcacggag gagacggata caatgcgatg
     3601 ataaacaccg gagtcaaggt aagttactgg aatgtctatt cagttctttc tgtcggcact
     3661 gacttcagca caattgcggc tcgtcctctt tcgcttggag tgactgccgg cggattttac
     3721 agaataagtg atttttccat aactgcagat ctgggatatg aaaaagtttg ggattttggt
     3781 gataatgcgg caataaacga ttatgctctc ggtttaaggg cagggctcag ttacagtgtc
     3841 ttaagctggt ttgcgataag tgccggggca ggggttaatt acagtatttt taaagattcg
     3901 agttttgagg aaggtaaatt tatcccaatg tttttcggtg gtttcgagtt cagcttaata
     3961 aaatgatctt ttttctgtaa ccaataattg acaactcagt ctaataattg agatggctga
     4021 aaaacatcca cagtttttat tcagtcgcct cgtatctgga tgggttgatc acacattgcc
     4081 ggctgatgca agtcagccgg ttcttttttt ttaagaagat tccagaattc ctgatgaaat
     4141 gcaaaattcg ataaattcag caacttcacc tttgtattgg agttttaaag cttcaatgac
     4201 ccgggaaagt gtgttttctc cattgaattt tctcaaaagc cgtgcccact tcgggtcaag
     4261 tgctgtgaaa atgctttcgc cgtttgaatt ataagtcacg acttctgttt tttgcggtct
     4321 gaaatgtttc acgaagtctt tcagtgtcat gtgtccgata tttaagagat catcgggatc
     4381 ataatcaaga gttatgagaa atgttccttt tgaagcagca agtatctgat tctgcggtgg
     4441 ttttgtgctt ttttctttca aagttgcaaa tcctgcctca agagacctgt cgagagaatg
     4501 gttaagccgg ataatgtttt ccatcggttt gtaaagagtg atcaaattgt gactttcaaa
     4561 aagctttttc acgaaatcaa gttgcagttt ctcaatatct aaacggtcac gataggatag
     4621 aaaggaggca aattcttcaa aaaagtccga tcctgaaatc tttaatgttt ccagtatcat
     4681 gaacagccat ccgacagatt ttccctgatt atagaaaaca ttgcagccat cggtgataac
     4741 ggcagcggct tcaatatctt cttttgaaaa tgagggagtt gatataacgg tgtagggagc
     4801 cgattgagaa aagtttatac cgaaagaatc cgctttttcg tgaattacag tgcctggaaa
     4861 tatcgcaaga ggaaaaatat caagattgtt aggttgaagt gaaactgcaa aatcaatgct
     4921 ttttttaaac ccggcaagag tgtcaccggg aagcccgtag atgagatcaa gtccgaaaac
     4981 gatattttca gaattgagta ttttgatctt ttctttgaaa tttgaaggat ttatgaatct
     5041 gtttacattg gacagaactg ccgggtcaga actttgaagc cctatctgaa gaccgcagtt
     5101 gaccgatttg aaaagttttg ccatttcacg atctaaaaat tctgttctga cttcaaaagt
     5161 aaagtgtatt tcaggggcgg tttgagcgat cattttcagg atcttttttg cccgttctct
     5221 atttttgttg aaagtgggat caaggacaaa gacctgagaa atattatgtt tttcaaacag
     5281 ttccagctct gctttaatcc tttcaagtga aaatgttctt acattggaaa cgcctcgtga
     5341 ttcaaaacag aaatcacatt tgaaaggaca tcctctcgat agttcccata aaactccgcc
     5401 gttgttttca agcttgacag ttccgttcag gaacggggag ggaagttcat ttatgtcagc
     5461 aaccggaaca ctgaaaatag actgttcaag tggtttgtta ttaaaaattc tgccgattgc
     5521 ttcgacagtt gcaagttcac cttctccttt aatgacaaaa tcaaaaagcc ctgaatttat
     5581 tagcttttct ggtgatgatg atggctctgc gcctcctgca aaaaggtatg tttcaggaga
     5641 tctgtcctta attattccgg caattttcat tacatgagct gtattccata aatacgttga
     5701 aaaccctact gcgtctggct tgcgaattaa aatagaatca gcaataatat caatgtgttg
     5761 atccagataa taatcaaagc atgaaacatt gagcaggtct ttcaattcac ttgtggaatc
     5821 aattgctgct tttatcattg ccgtccccag agccattgcc tgaggcgatt tttttatgtg
     5881 tattgagatc agatcgattt tcatgccgtc tcccaagaaa ttcaactgca atatacacat
     5941 atttgttttt tcttgaaact tttttaagtc attatagaat gcattgttgt tgttgttttg
     6001 gaacaataga gaaacatgag attcaagatc atcagagatg tgacaattct taaaaatata
     6061 agtccgttga gaggcaattc agattacgcc tactcaaatt agtttttgaa ataaatccaa
     6121 gctgttatat taattattgt ggagactgcc atgaatttac cttatgcgga acctttcagg
     6181 atcaaagttg ttgaaccttt gaaaacaagc acgaaggaag aacgggtcaa gtggatcaaa
     6241 gatgcccgtt tcaatctttt caaccttaga agtgagcagg ttttcattga tctgcttact
     6301 gattcgggaa cgggctcgat gagtagtcat cagtgggcgg aaatgatgct cggagacgaa
     6361 agttatgcgg gagcgtcatc attttataag ttaaaggaaa ctatcgggga gcttctcggg
     6421 tttccttatt ttcttccgac acatcaggga agagcagcgg aaaatgttct gttttcagct
     6481 atgatcggaa aagacggaac aggcaaagtg gttcccggca acagccactt tgacacaaca
     6541 aaaggacata tcgagtttcg gaacgcaaga gcgatagatt gtacgatcga tgaagctttt
     6601 gatacaacgg tcgatcatcc gttcaaggga aatatcaatc ttgaaaagct tgaaaaagtt
     6661 tttattcagt acggaaagga caacattcct ctttgtataa ttactgtcac ctgcaactca
     6721 tcagggggac agccggtttc aatgcagaat ataaaagatg tcagtgcact ttgcagaaag
     6781 tacggcataa cagttatttt tgatgccgca agatttgctg aaaacgccta tttcatcaag
     6841 atcagagaag acggatatca gaacaagacg ataaaagaga tcgtttatga aatgttcagt
     6901 tttgttgacg gctgtacaat gagttcaaaa aaggacgggg tagtaaatat cggtggtttc
     6961 ataggactga gaagcaggga gctgtttgaa aaagcgtctg ttttcaacat tgtatatgaa
     7021 ggttacatta cttacggcgg catggcggga agagacatgg gagctctttc cgtagggctt
     7081 aaggagtcaa cggagtttga ttttctaaga cagagaatag gtcagataga gtatcttgga
     7141 aaattgctca aagattacgg tgtgcctgtt caggaacctt tcggcggtca tgcaattttt
     7201 gttgatgcaa agagattcct ttcccatatt cctaaagagc agttcattgc tcagacattg
     7261 ggtgttgaac tttatatcga agggggtgtg aggggtgttg agatcggaac tcttcttgct
     7321 gacagagatc cggatacaag agagaacaga tatccgaatc ttgaactttt gagactcgca
     7381 gtgggaagac gactttatac agataaccac atggcatatg tcgccgcggc acttaaaaat
     7441 gtttatgaca gaagaaatga aataaaaaca ggtctgaaaa ttattaaaga agctcctata
     7501 atgagacatt ttacagttga gcttgaaaga gcataattca aaaaggagag aaaattgtcc
     7561 gaatttaaaa agcttgttga ttcgccggca gggagccggt tcattgttca gggaaacacc
     7621 gcttttgcgc ttggtgttgt ccatgccggc tatcatgccg ctgacggcta tccgggaact
     7681 ccgagcacgg aagttattga taaaaatctc aaatatgttg ctgacagaat gactgtcgga
     7741 tggagcgtca atgaagctgt ggcgactgct gtaacgatcg gtcacagcgt tgcaggaatg
     7801 gatgcccttg tcacaatgaa gatccccggt ctttttcagg caggcgacac aattaccact
     7861 tccgctttct acacgggaca gtcaggagca tttgtcgttt atgcggctac tgattatgtt
     7921 ccctcttcaa cccagcatgt gatcgatgca agatattttc tttctgctgc aagaattcct
     7981 gttcttgagc cgagagatca tcaggaaatg tatgatattg cgtttttagc agcagacatt
     8041 tcaagaaagt tcagaactca ggtatgcgtt cttgcaagcg gaatattagc tcacagcgaa
     8101 gggcttgtga caaccagaga gccaagaaag atagagccga gaccgcttcc tcaaaatctt
     8161 catgagtgga tgctaatgcc gggtattgca agaaaaaact ataacgaagt caataaggtt
     8221 agaattcctc aactgcttga atatgcagag aaaagcgaag attttatgag tgagcatatt
     8281 gggactgatg actggggagt aattgttgtc ggagaaagcg acataattgt aaaagaggca
     8341 tttgttacga ttaaagcaga tccttccatt ctttcaatgt caattgcaaa ccctgttccg
     8401 gtgaatagga tcagggaatt tgctgcaaaa gtgacggggc ctctttttgt ttttgaagac
     8461 ggcgatgttt ttctgcagga aaaaattgca gtggaaggga tcaaagtgat cggcaaggag
     8521 aaaaattcgg taattaccga ctggactcct tttgaagtga taagttttct taaaaaacat
     8581 attcagatca gtttcgatat aactcctttg aaaattgatg tgaaaccgtt aatgagaccc
     8641 ccttcaatat gccctgggtg tccttataaa gcttatggtc ttgcggcaga aaaccttagg
     8701 aaaaaaggga agttatatgc agggttcggt gagattggct gttcgacgct cctttatttc
     8761 aacaagggta tcgacacaat tctctgcatg ggcggaagtg atgcgatgag gcagggtttc
     8821 tgcatgagca gacctgaaat ggtttcaaaa gttgtgagcg tcatcgggga cagttgtgag
     8881 tgtcacagcg gtcttgatgc gactcgaaac ggcgttttca ggaatgttcc gggagttcag
     8941 gtcgtacttg ataacagaat tacagcaatg actggcggtc agcccgctcc gacaagtgag
     9001 atgaaccttg caggagttcc aaataagttc aacctcaaaa aagcaattgc ggctgaagag
     9061 tgcaatgttg ttgcagtgaa tgcctatgat ctcaaaggtg ttgaaaaagc acttactgaa
     9121 gcacttgatg aagctgaaag aggtcagttc acaaatcttg ttcttgaagg ggcatgtcag
     9181 cagatagttg acccgaaact caaaaagaga actatccgtt ttgacaggga aaaatgcaaa
     9241 aaatgcggaa gatgcaacat ctgtcccgga ataaaattcg gcgaagacgg ttatccggaa
     9301 ttcacgaatc tctgtatcaa ctgcgccgga aatacacagg tctgcatgca gagatgccct
     9361 ttcggagcaa tcgttccgat tgatgaagaa agcaggaagg tgtctgaaaa gattgaactt
     9421 ccggctccca aggtttttac atcggcgaca gtgaaaaagg aagatcttcc cgaatcatta
     9481 aggatagcga tcagaggaat aggtggacag ggaaacctct ttttcggcaa ggttttaagt
     9541 gaagttgcgc tccgaactcc atacagcgag gcgagaattg taaaaggtga cactcacgga
     9601 atggctcagc ttggcggaag cgttatttcg acatttgcct gcggaaacgc atattcaccg
     9661 atactttctc cgggaagcac tgatattctt gtcgtcatgg aaacttcaga agtgctcaga
     9721 gaagggttcc ttgaacttct caaaccgggc ggcacgatcg ttctcaacag gtttgaagca
     9781 gttcctccta cagcaaaaaa agaagattat cccaaatttg atgacattaa aaaagcgctg
     9841 tcaaattata aagttataga atttgatgca ctcgaaactg tttcaaaact tggagacaaa
     9901 ctcggcagaa ccgcaaatgt cgcagtgctc ggactccttt ccaccatcga accgcttaac
     9961 aaaataccgc ttgaaatatg gatgtccgca ttgatgaaac tttcacccaa agattcaata
    10021 agatcagcaa atcagatagc ttttgaagca ggaaggagtg caaaatgagc aaagttctaa
    10081 tcggttattt ttcaaagacc ggatcaacaa aggagatcgc agaaagaata ggtgcaaacc
    10141 ttgcgaaaaa aggtgttgaa agcgatgttc tggaactcga aaagatcaaa aatatagaga
    10201 attattcaag tgtcatcctc ggagcgccca taaacggaat gaagcatgtg cccgaattca
    10261 ttaaatttat tgaagaaaat aaaaccgctc ttgatggtaa agtcaaagga cttttcacag
    10321 tcgcctacat ctattacgac ggcagaagtc tgtggcgttc attcataaat aaaagcatca
    10381 cgaaaattga aaagctcatc aaccccaaaa actcaattgt ctttgccggc agaatcgaca
    10441 agaaaatgcc agcattcgcc aatcttctct tcggcatcgg cagtgacctc cccaacgacc
    10501 tcatcaacca tgaagaaatc gacaagtgga gcgaaggggt ttgatgctcc gcttaatgtt
    10561 ccccgtaatg ttcgtttttg gtgttgcggc ggcctgtttc aagatcatga tcgcgttcgc
    10621 ttgaatcttt tttgaacggt gcaagactgt tgacccttat ctgcttgcct ttataaacaa
    10681 attcagtccc tgagaactca agtctctgaa ggacaaaaat atcacc
//
LOCUS       DAOOYB010000090        10579 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_6742_length_10579_cov_9.664196, whole genome shotgun sequence.
ACCESSION   DAOOYB010000090 DAOOYB010000000
VERSION     DAOOYB010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10579
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6742_length_10579_cov_9.664196"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(159..2207)
                     /locus_tag="PL056_06845"
     CDS             complement(159..2207)
                     /locus_tag="PL056_06845"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HPM46768.1"
                     /translation="MKRSLFTVLCAFLLFSVSCASAGDTKQTEVKPAETKAQEKPAED
                     FGMVGNAYLKLCRNEIASISGIASEIKTSAGKKSGLEILNLMNLLEIKQSDMWSKSGI
                     FQLNHPDEGMRKAAMKCETEISAQATELSLDNELYKAIASISPGDPALDSDDKYFLAD
                     MIIDFKRNGVDKDEATRDAIKKLSAEIIALEQTFYSNIGSDKKTIKIKDVNRLKGLPE
                     DFIKGKKPDEEGNIILTTDWPDYFPVMENAQDESLRAEMYKNVLNIAYPQNTEIFSKI
                     LKARKKFSELLGYKNFAEYTQQKLMIENPENADEFIKKVAGISTAYAKKDLEVFLAKK
                     KSVVGETAEVEPWDRFFYQKLIKMEKYNYDPEQARKYFEMTKVIDGIFLVSGKIFGVT
                     FEKVKRDDLWHESVQSYNMLADGKVIGMFELDLFPRPNKYKHFAMFTNELGVKDLRIP
                     RAAIIGNFPEPVDGKAYISHEDVQTLFHEFGHLLHSLFAGNQRYVRFSGTNCQRDFVE
                     VPSQLMEEYVWDASILQLWATNDAGEPIPADLVDNMKKASEFAKGLHVSRQMYLAALS
                     LGIYLQDPENFDHHAYEKKLEKELSPWKSHEDTHLIENFSHLVNYYSNYYTYMWSLAI
                     VKDFAGHIRKTGLMDAAVTKQYRDKVLNIGGAKPGHQMVKDFLGRELSFEEFEKWLNN
                     "
     gene            complement(2219..3247)
                     /locus_tag="PL056_06850"
     CDS             complement(2219..3247)
                     /locus_tag="PL056_06850"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003369397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HPM46769.1"
                     /translation="MVAKADIKWSELRFGYIKCDYRFISHFKDGKWDEGELTKDDYIR
                     IHEGSTAIHYGQQCFEGMKAYTTKEGKILLFRPDRNAARMKNSCEQLLMAVYPEEKFI
                     EACIKVVKANERFVPPYGSGASLYLRPYLIGVGENVGVRPAPEYLFGIFVTPVGPYFK
                     GGMAPCHFVISEYDRAAPRGTGGVKVGGNYAASLKAHKVAVDAGYADCIYLDPATHTN
                     IEEVGAANFFGITADNSFVTPKSPSILPSITKYSLMHLAEHNLKMKVVEKEIPVTSLG
                     EFVEAGACGTAAVITPIGSITHEGKKYAFYGEGKEVGPKIKELYSLLTSIQTGDIKGP
                     DGWVVEVK"
     gene            complement(3353..>5287)
                     /locus_tag="PL056_06855"
     CDS             complement(3353..>5287)
                     /locus_tag="PL056_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lectin domain-containing protein"
                     /protein_id="HPM46770.1"
                     /translation="GTLWNTVSSYEQIWDGTNWIPAESTTEYNAEPSSEHCRFKCDTN
                     YTWNSGTSTCEANTKPFTCDPKPATGTIWNTVDSYTQTWDGGKWVPDDSDTEYNETAS
                     SSACRFKCGDNYTWNSLMCVADSKPFNCAEKPGGNTTVWNTVSGYTQTWSGTAWLPAD
                     SSTTYNGTASATECRYKCANNYTWNSGSSTCVANTQTANCTGLPSNATWNNVSSINQT
                     WNGTDWAPSSTGTYNITPSSTECRFVCNVNFNWNSPNCVAATQTFSCTAKPGGETTMY
                     NTVSSYTQTWNGSGWLPADDEVTEYNETGSLTSCRYKCANGYTREGENCVINTKTFTC
                     ADKPGGTTTVWNTVSSYTQTWNGANWLPADSTTAYNETGSDTSCRYKCADNHVWDGSS
                     CLACASGWELMNLGTPRCIKAFTDTRTADSARAQCMTFNTGRLVTIRNASENEFVRSK
                     LGANSWIGLMFYNGRSVNPTVNNSSATPIWLGNYGGVAVGDTGSSPSGNRFHYLKFTT
                     KEAALYSASLWGMSDDLDLYLYDANCSSVITKSETYGDELLFATLSANTTYCVRIGYS
                     GYSSAYIVAVNYAAREEEQYKDWVSNGIDMPLYNYHNWNSGEPNNDDELCTQMYTTGF
                     WNDLDCSDSLQYVCERNPGS"
     assembly_gap    5288..5387
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5388..6554)
                     /locus_tag="PL056_06860"
     CDS             complement(<5388..6554)
                     /locus_tag="PL056_06860"
                     /inference="COORDINATES: protein motif:HMM:NF019271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding EGF-like domain-containing
                     protein"
                     /protein_id="HPM46771.1"
                     /translation="MSFRIIFFTSVLVIVFSACSSENKETPECITAFDCLDGQICVDG
                     KCIKGEAGNTGDTGNTGDSGNTGNTGNTGNSGNSGNTGNTGNSGNTGNTGNTGNTGDT
                     ATDPCDPNPCLGISFSDEICTVEGENYSCGCVEGYFWNESLCDDVDECSEHLLNNCSE
                     FADCTNITGSYECECKENYTGDGFDCVADTLQVNCENALPDNASWSSSNPDGSLNQIW
                     DGEKWAPSADECAWECNENYTKSDQTCVADTKTFTCTGKPGSITTVWNSVDKYTQTWD
                     GSVWVPADSATEYNAESSETACRYACAENYTWNGTVCSANTKIYVCPEKPEPGTEWNS
                     VDSYTQTWSGSAWLPAGSSTVYNTTASAFSCRYKCAENFTWNGTICAANTKIFTC"
     gene            complement(6586..>9043)
                     /locus_tag="PL056_06865"
     CDS             complement(6586..>9043)
                     /locus_tag="PL056_06865"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EGF domain-containing protein"
                     /protein_id="HPM46772.1"
                     /translation="SDSDNTNPCDPNPCLEIALSDEICTAEGENYSCGCVDGYFWNEE
                     KCDDIDECVDGILNDCSESADCSNTTGSYECTCRENYSGDGFECVADTKDVNCENTFP
                     ENAAWNSENPEGMLTQTWNGTDWAPAADQCKWDCIADFAKNDAEDGCINSRNVSCTNI
                     PANASGSGDNSDGKIFQTYTDLTGWTPGADTCSWNCNTDYVLNETSDGCINSKLAPCS
                     PNDILNSTDATDAVIITYTTAGGWTTPAKCSWTCNTDYEQNGNSCINSKLVSCDTNNS
                     NPANSTDIIANVTVTYTTVGGWIAPAKCDWTCNQYYKKSGLVCILACGEGNVDTGEAC
                     DSGTENADLEYNFTPTCNTNCQWNSYCGDGVENNAKNNLSQTIVTLYMDEGSGTTALD
                     SSGSGNHGSISNASWVTGKHGQALSFNGTDSVVSLSYTPPTNNFTMMAWIKASLTHEI
                     DPQQIDIVTGTTGQNYVFGADHRSEFAGAGVSAGTNGVSVYEHGDGYMPALAVYSGSI
                     GTGWVHVAVTYADRQPKIYINGNLVVTGLVSAKSTVFAPTKIGGGSYGYFNGAIDGVK
                     IIERAVTAEEIKSAMSEACDDGVLNGTSGKCKIDCTGYECVPGTYTFEYTGWEQSFIV
                     PGPGTYLVEAWGGQGRTNGAGAVAGAYGGYAKGTLAVTTGETLYIYVGEGGGTSFAGG
                     WNGGASGGSSPSYPASNGGGGGGAWGGTGGGGGTQSGGGEAGLFGYIGNSGIAGTYGA
                     GGAGGNVSENSNQSDSNTAPGGGTGGGLTGISGGSGSTKYTGGGGGGGSGYTGGVTNG
                     SMQNGIRTGNGRVVITRTCP"
     assembly_gap    9044..9143
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9144..9458)
                     /locus_tag="PL056_06870"
     CDS             complement(<9144..9458)
                     /locus_tag="PL056_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46773.1"
                     /translation="MPIIFRISIFIVFVFAFIFGCSSDKTQYCITAFDCDAGQICVDG
                     FCVDKESGNTGNSGDSGDSGDSGNSGNTSDQSDQSDQSDMSDMSDSDNTNPCDPNPCL
                     EIA"
     gene            complement(9465..>10579)
                     /locus_tag="PL056_06875"
     CDS             complement(9465..>10579)
                     /locus_tag="PL056_06875"
                     /inference="COORDINATES: protein motif:HMM:NF024220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine rich domain-containing protein"
                     /protein_id="HPM46774.1"
                     /translation="LTLFLYSCSGSESSYCITAFDCKEGEICRNGECIPGTAGDSGNS
                     GDSGNIGDSGNSGNSGNSGNSGDSGNSGDSGDSGDSGDSSDDDETVVNPDNTYSDNET
                     DYETTDITEDEISDIDADESIDETIIDDDPPPCVPGTFTFEYTGAEQTWTVPSGTGTY
                     TAEVWGAQGGQGSADGIAQGALGGYAKGTLSVTSGQILYVYVGQGNVYSTTTLVSAFN
                     GGGAGRGNSDTNTRGAGGGASDIRIDGNALANRVIVAGGGGGSSGTSYIGGAGGGVSG
                     SDGTGGLPGTGGTQTAGGISAFEAEMTHGSLGQGGGLTEGSTFTLAGGGGGYYGGGGG
                     LGAGGGSGYIGGVTEGTMQNGVNSGNGKVVITRTCP"
BASE COUNT         2602 a         2477 c         2056 g         3244 t
ORIGIN      
        1 aaaagactct ctgtttttat ctcagatttt ttgggggaac ttcaaaatcg gttaaaaagc
       61 atagaccttc acacatttga atcttcccac atattcttca aatgttgaac acaacgccgc
      121 cgttgaacac tatcaggtca aaggactcag aacgaagttt aattattaag ccatttttcg
      181 aattcttcaa aggagagttc gcggccgagg aaatctttca ccatctgatg cccgggtttt
      241 gctccgccga tgttgagaac tttatcgcgg tactgttttg ttactgcggc atccataagc
      301 cctgtctttc tgatgtgacc tgcgaaatct tttacaattg caagtgacca catgtaggtg
      361 taatagttcg agtagtagtt tacaagatga ctgaaatttt ctatcagatg tgtgtcttca
      421 tgggatttcc atggtgaaag ttctttttca agcttctttt cataagcatg atgatcaaag
      481 ttttcagggt cctgaaggta tattccaagt gaaagtgcgg caagatacat ctgtcttgaa
      541 acatggagac cttttgcaaa ttcacttgcc tttttcatgt tgtcaacaag atcagccgga
      601 atcggttcgc ctgcatcatt agttgcccag agctgaagga tcgatgcgtc ccagacatac
      661 tcttccataa gctgtgacgg cacttctaca aaatcccgct gacagttggt tcctgaaaaa
      721 cggacatatc tctggtttcc tgcaaaaaga gaatgaagca gatgtccgaa ttcatgaaag
      781 agcgtttgaa catcttcatg agaaatatat gcctttccgt caaccggctc agggaaattc
      841 ccgattatcg ccgctctcgg gattcttaaa tccttaactc cgagttcatt tgtgaacatc
      901 gcaaagtgct tgtatttatt gggtcttgga aacagatcga gctcaaacat tcctatcact
      961 ttaccatctg caagcatgtt gtatgactga acactttcgt gccacagatc gtctcttttc
     1021 actttttcaa atgtcactcc gaaaatcttt cctgaaacaa gaaatatgcc gtcgataacc
     1081 tttgtcatct caaagtattt tctcgcctgt tcagggtcat agttgtattt ttccattttt
     1141 atcagtttct gatagaaaaa cctgtcccac ggttcaacct cagcagtttc tccaacgaca
     1201 gatttctttt ttgcaagaaa aacttcaagg tctttttttg cataagccgt tgaaattcca
     1261 gcaacttttt tgataaattc atctgcattc tcagggtttt ctatcataag tttttgctga
     1321 gtgtattctg caaaattctt gtatccaaga agttcagaga atttctttct ggctttcagt
     1381 atctttgaaa atatctctgt attctgagga tatgcgatat tcagaacatt tttatacatc
     1441 tctgctctga gagattcatc ctgagcattt tccataacag gaaagtaatc aggccagtct
     1501 gttgtaagaa taatattgcc ttcttcgtcc ggttttttcc ctttgatgaa atcttcagga
     1561 agtcctttaa gacggtttac atccttaatt tttatggtct ttttgtcaga gccgatgttg
     1621 ctgtaaaatg tctgttcaag tgctattatt tcagcactga gcttttttat cgcatctctt
     1681 gtcgcttcgt ctttatcaac accgttcctt ttaaaatcaa tgatcatatc ggcaagaaag
     1741 tatttatcat cgctgtcaag agcgggatct ccaggagaaa tggaagcgat cgccttataa
     1801 agttcattgt caagagaaag ctctgttgcc tgagcactga tctccgtttc acatttcatt
     1861 gctgcttttc tcatcccttc gtcaggatga ttaagctgaa atattcctga ttttgaccac
     1921 atgtcacttt gttttatttc gagaagattc atcagattaa gaatttccag tccgcttttt
     1981 ttaccggcag atgtctttat ttcagaagca attccagata tagatgcgat ctcgtttctg
     2041 caaagtttta aataagcatt gccgaccata ccgaaatctt cggcaggttt ttcctgtgct
     2101 tttgtttctg ccggtttcac ttcagtttgt tttgtgtctc ctgcactggc acatgaaaca
     2161 gaaaacagaa gaaaagcaca aagcactgta aataatgatc ttttcaattg aacctccttt
     2221 atttgacttc aaccacccag ccgtcaggtc ctttaatgtc cccagtctgt attgaagtaa
     2281 gcagagaata gagctctttt atttttggac caacttcttt tccttcaccg tagaaagcat
     2341 attttttccc ttcgtgagtt attgaaccga tcggagttat gacggccgca gttccacagg
     2401 caccggcctc gacaaattct ccaagagagg tgacagggat ctctttttcg acaactttca
     2461 ttttgagatt gtgttcagca aggtgcatca gtgagtattt ggtgatggaa gggagtatgg
     2521 aaggggattt cggggtaaca aacgagttgt cggcagttat tccgaaaaag tttgcggcac
     2581 ccacttcttc aatgtttgtg tgagttgcag gatcaagata aatacagtcg gcatatcccg
     2641 cgtcaactgc aactttatgc gctttcaagc ttgccgcata atttccgcca acctttactc
     2701 cccctgtgcc tcttggagct gcacggtcat attcagaaat aacgaaatga catggagcca
     2761 ttcccccttt aaaatagggt cctacagggg ttacaaagat tccgaaaaga tattcaggtg
     2821 caggtcttac accgacattt tcaccgacac ctataagata tggtctgagg tataaagagg
     2881 caccgctgcc gtaagggggg acaaatcttt catttgcttt taccaccttg atgcatgctt
     2941 caatgaactt ctcttcagga taaacagcca tgagaagctg ttcacatgaa tttttcattc
     3001 ttgccgcatt tctgtcaggt ctgaaaagga gtattttgcc ttcttttgta gtgtatgcct
     3061 tcatcccttc aaaacactgc tgtccgtaat gaattgctgt tgaaccctca tgaattctga
     3121 tgtagtcgtc ttttgtaagt tcgccttcat cccatttgcc gtccttgaaa tgtgaaatga
     3181 aacggtaatc acatttgatg taaccgaaac ggagttccga ccatttgatg tcagcttttg
     3241 caaccatttt tacctccaga attttattat taatttagtt gaaaaaaaac gtaatgcaaa
     3301 agactttaga aaataaaaag aggtttgtca accttgacag ggatgaagaa ttttacgatc
     3361 ctggatttct ttcacacaca tactgcaaac tgtctgagca gtcaagatca ttccagaagc
     3421 cggtagtata catttgagtg cagagttcat catcattgtt gggttctcca ctgttccagt
     3481 tgtggtaatt gtaaagaggc atgtcaattc cgtttgaaac ccagtcttta tactgttctt
     3541 cttctctggc agcatagttt actgcaacta tataagcact tgaatagcca ctataaccga
     3601 ttctgacaca gtaagtggta tttgccgata aggttgcaaa caaaagttca tcaccataag
     3661 tttctgattt tgttataaca gagctgcaat ttgcatcata taaataaaga tcaagatcat
     3721 ccgacattcc ccaaagtgat gcagaataaa gggcggcttc ttttgtagta aatttaagat
     3781 aatgaaatct gtttccgctt ggtgaagaac cggtatctcc aacagccaca ccaccataat
     3841 ttccaagcca gataggagtt gcagaactgt tgttaactgt cggatttaca gatctgccgt
     3901 tataaaacat cagtcctatc caggaatttg cacccagttt tgatctgacg aattcatttt
     3961 cagaagcatt tcttattgtt acgagtcttc cagtattgaa ggtcatgcac tgagctcttg
     4021 ccgaatcggc ggttcttgta tctgtgaatg ctttaataca tctcggtgtt cctaaattca
     4081 tcaactccca acccgatgcg caggcaagac agctcgaacc atcccagaca tgattgtcgg
     4141 cacatttata tctgcatgat gtgtcgcttc cagtttcgtt gtatgctgtt gtgctgtctg
     4201 caggaagcca gtttgctccg ttccaggtct gagtgtaact tgaaacagtg ttccacacag
     4261 tcgttgttcc acccggttta tcagcacagg tgaaggtttt tgtgttgatg acacaatttt
     4321 ctccttctct ggtataacca ttggcacatt tatagcgaca tgaagttaaa cttcccgttt
     4381 cgttgtattc agtcacttca tcatctgccg gcagccatcc ggaaccgttc caagtctgag
     4441 tatagcttga aaccgtgtta tacatcgttg tctctccgcc cggctttgca gtacagctaa
     4501 atgtctgagt tgccgcaaca caatttggac tgttccagtt aaagtttaca ttacagacaa
     4561 acctgcattc agttgagctt ggagttatgt tatatgttcc ggtgctggac ggagcccaat
     4621 cggtgccgtt ccaggtctga tttatgcttg aaacattgtt ccatgtagca tttgacggaa
     4681 gaccggtgca gtttgctgtc tgagtattgg caacacaggt cgaagatccg gaattccatg
     4741 tatagttgtt tgcgcatttg tatctgcatt ctgttgcact tgctgttcca ttgtatgttg
     4801 ttgaactgtc agcaggcagc catgcagttc cgctccaggt ctgagtgtat cctgagacgg
     4861 tgttccagac agttgtgttt ccgcccggtt tttcagcaca gttgaaaggt tttgaatctg
     4921 caacacacat caagctgttc catgtgtagt tgtcaccaca cttgaatctg catgcggaac
     4981 tacttgctgt ttcattatat tctgtgtcac tatcgtcagg aacccatttg cctccgtccc
     5041 atgtctgagt atagctgtca acagtgttcc agatagttcc tgttgcaggt ttagggtcac
     5101 atgtaaaggg ttttgtgttt gcttcacagg ttgatgttcc ggaattccat gtatagttgg
     5161 tgtcacattt gaatctgcag tgttcactgc ttggttcagc attatattcg gttgtgcttt
     5221 ctgcgggtat ccagtttgtg ccgtcccaga tctgttcata gcttgataca gtgttccaca
     5281 aggttccnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaca tgtgaaaatt
     5401 tttgtgtttg cggcacagat cgttccgttc caggtaaaat tttcagcaca tttatatctg
     5461 cacgaaaaag cgctcgcggt agtgttgtaa actgttgaac tccctgcagg aagccatgcg
     5521 gagccgctcc aagtctgagt ataactgtca acagagttcc attcggttcc cggttccggc
     5581 ttttcaggac agacatatat ttttgtgttt gctgaacaga ccgtgccgtt ccatgtataa
     5641 ttttcagcac aggcatatct gcatgcggtt tcactcgatt cggcattgta ttctgtggcg
     5701 gaatccgcag gaacccatac cgaaccatcc catgtctggg tatatttgtc aacgctgttc
     5761 cagacggttg ttatagatcc aggttttccg gtacatgtaa aagtttttgt atctgcaacg
     5821 catgtctgat ctgatttggt gtaattttcg ttgcattccc atgcacattc gtctgctgac
     5881 ggagcccatt tttccccatc ccatatctgg ttgagcgatc cgtcaggatt tgatgaactc
     5941 catgatgcat tgtcaggaag agcgttttca cagttgactt gtaatgtatc tgccacacaa
     6001 tcaaatccgt caccggtata attttcctta cactcacatt catatgagcc ggttatattt
     6061 gtgcaatcag caaactcgct gcagttgtta agcagatgct cactgcattc atcaacatca
     6121 tcacataaac tttcattcca gaaataacct tcaacacagc cgcaggaata attttcacct
     6181 tccacagtac atatttcatc gctaaatgaa attccaaggc aggggttcgg gtcacaagga
     6241 tcggttgccg tgtcgcctgt gttcccagta tttccagtat tccccgtatt gccgctattt
     6301 cctgtgttgc ctgtgtttcc tgaattacct gagtttccag tatttcctgt gttgccggta
     6361 tttccactgt ctccggtatt tccagtgtct ccagtgtttc ccgcttcgcc tttaatgcat
     6421 ttcccatcaa cgcaaatctg cccgtcaaga cagtcaaaag cggtaataca ctcaggtgtt
     6481 tccttgtttt ctgaagaaca tgctgaaaac actataacca gcactgatgt aaaaaaaata
     6541 atacgaaaac tcatttttcc ctcgcaagta taaaaaaatt aaatcttaag gacaagtcct
     6601 tgttataacc actctcccgt ttcctgttct gataccgttc tgcattgatc cgttagtaac
     6661 tccgcctgtg tatcctgaac cgccgccacc gccaccgcca gtgtattttg ttgaacctga
     6721 accacctgaa atacctgtca atccaccacc tgtaccgcca ccgggagcag tattggagtc
     6781 tgattgattg ctgttttcac ttacatttcc gcctgctcca cctgcaccgt atgttcctgc
     6841 tataccacta tttccgatat atccaaacaa acctgcttcg ccaccacctg actgtgtgcc
     6901 tccgcctccg cctgtgcctc cccatgcacc accgccaccg ccaccgtttg atgcaggata
     6961 agatggggat gatcctccgc ttgcaccacc gttccagccg cctgcaaagc ttgttccgcc
     7021 accttcaccg acataaatat agagagtttc accagttgtt acagcaagag ttccttttgc
     7081 atatccaccg taagcaccgg ctacagcacc tgctccattt gtgcggccct gtccgcccca
     7141 tgcttcaaca agatatgttc ccggtcctgg aacaataaac gactgttccc atccagtata
     7201 ttcaaatgta taagtgccgg gaacacattc atatccggtg cagtcgattt tacattttcc
     7261 tgaagttccg tttaaaacgc catcatcgca tgcctcagac attgcagact ttatttcttc
     7321 agcagttaca gctctttcaa tgatctttac tccatcaatg gcaccattaa aataaccgta
     7381 agaacctcct ccgatcttgg taggtgcaaa aactgtcgat tttgctgaaa caagtcctgt
     7441 cacaacaagg tttccgttaa tgtatatttt cggctgtcta tcggcataag ttactgcaac
     7501 atgaacccag ccggtaccga tacttccgct gtaaacagca agtgcaggca tatatccgtc
     7561 tccatgttca tagaccgaga ccccgttcgt tccggctgaa actccggctc cggcgaattc
     7621 actacggtga tcagctccga aaacataatt ctgtccggtt gttcctgtta caatatctat
     7681 ctgctgggga tctatttcgt gtgtcaaaga cgctttgatc catgccatca ttgtaaaatt
     7741 gttcgtcggt ggagtgtagg aaagggaaac aacgctgtcc gttccgttaa aactcaacgc
     7801 ttgcccatgt tttccagtaa cccacgaggc attgctgatc gaaccgtgat ttccactacc
     7861 cgatgaatca agagccgttg ttccgctgcc ttcatccata taaagggtca caatggtctg
     7921 acttaaatta ttttttgcat tgttttccac cccgtctccg cagtaactgt tccattgaca
     7981 attcgtgttg caggtagggg tgaaattata ttctaaatcg gcattttctg ttccgctgtc
     8041 acaagcttct cctgtatcaa catttccttc tccgcaagcc agaatgcaga ccaacccgct
     8101 ttttttgtaa tactggttgc atgtccagtc acattttgca ggagcgatcc atccgccgac
     8161 agttgtataa gtcacagtca catttgctat tatatcggtt gaatttgcag gattgctgtt
     8221 gttcgtatca caagaaacaa gttttgagtt tatgcatgaa tttccgttct gttcataatc
     8281 agtattgcaa gtccatgaac attttgcggg cgttgtccag ccgcctgccg ttgtataagt
     8341 tatgatgacc gcatcagttg catctgttga atttagaatg tcgtttggag aacatggagc
     8401 aagcttggaa ttgatacagc cgtcagatgt ttcgttcagt acataatcag tattgcagtt
     8461 ccatgagcag gtgtctgctc ccggtgtcca gccggttaga tcggtatatg tctggaaaat
     8521 ttttccatct gaattatcac cgcttcctga agcatttgca ggaatgttcg tgcaggaaac
     8581 atttcttgag tttatgcatc cgtcctcagc atcgtttttc gcaaaatcgg caatacagtc
     8641 ccacttacac tgatctgccg caggagccca atctgttcca ttccatgttt gagtcagcat
     8701 tccttcagga ttttctgaat tccatgctgc attttcagga aatgtgtttt cgcaattaac
     8761 atcttttgtg tctgcaacac attcaaaacc gtcacctgaa taattttcgc gacaggtaca
     8821 ttcataagaa ccggtggtat ttgaacaatc ggcagattca ctgcagtcat taagaattcc
     8881 atcaacgcat tcgtcaatgt catcgcattt ttcttcattc cagaaatagc cgtcaacaca
     8941 gccgcaggaa taattttcac cttcagcagt gcaaatttca tcgcttaatg caatttcaag
     9001 gcacggatta gggtcgcaag gattagtgtt atcactgtct gacnnnnnnn nnnnnnnnnn
     9061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9121 nnnnnnnnnn nnnnnnnnnn nnntgcaatt tcaaggcacg gattagggtc gcaaggatta
     9181 gtgttatcac tgtctgacat gtccgacatg tctgactggt ccgactggtc cgactggtcc
     9241 gatgtgttcc ctgaatttcc ggaatctcct gaatccccgc tgtcaccaga gtttcctgtg
     9301 tttccacttt ctttgtcaac acagaatccg tcaacgcata tctgtcccgc gtcgcagtca
     9361 aatgcggtaa tacagtattg agttttgtca gatgaacatc caaagataaa agcaaataca
     9421 aaaactatga aaattgaaat tctgaagatt attggcacct ctttttatgg acaagttcgt
     9481 gttataacta ctttgccatt acctgaattt acaccgtttt gcattgttcc ttctgtaaca
     9541 ccgccgatat agcctgaacc accgccggca ccgagaccgc cgccgccgcc gtaatagccg
     9601 ccaccgccac ctgcaagtgt aaaagttgat ccttctgtta atccgccacc ttgtccaaga
     9661 gacccgtgag tcatttctgc ttcgaaagct gatatgcctc cggcagtttg agttcctcct
     9721 gttcccggta atccgcctgt gccatcactg cctgacactc ctcctcctgc tccaccaata
     9781 taagaagttc cggaagaacc gccacctcca ccggcaacga taacacgatt tgcaagtgcg
     9841 tttccgtcaa tacggatatc gcttgctccg ccacctgctc ctctggtgtt ggtgtctgaa
     9901 ttgccgcgac ctgctcctcc tccgttgaat gctgagacaa gggtcgttgt agagtaaaca
     9961 ttgccttgtc cgacataaac atacaaaatc tgtcctgaag ttacagaaag agttcctttt
    10021 gcataacccc cgagagcacc ctgtgctatt ccgtcagcgc ttccctgtcc accttgagca
    10081 ccccatactt cagcagtata agttccggtt ccagacggaa cagtccaggt ttgttccgca
    10141 ccggtatatt caaatgtgaa agtgcctgga acacatggag gaggatcatc gtctattatt
    10201 gtttcatcaa ttgattcatc agcatcaata tctgaaattt catcctctgt gatgtctgtt
    10261 gtttcataat ccgtttcatt gtcagagtaa gtattatcag gattgacaac tgtttcatca
    10321 tcatctgagg agtcgccgct gtcacctgaa tccccgctgt caccagagtt tccactgtca
    10381 cctgaatttc cgctgttacc actgttccct gagtttcccg aatcgccgat gtttcctgaa
    10441 tcacctgaat ttccagagtc tccggcagtg cccggaatac attctccgtt cctgcatatc
    10501 tcaccttctt tacaatcaaa tgctgtaatg cagtaactgc tctcacttcc tgaacaactg
    10561 taaagaaaaa gtgtcagaa
//
LOCUS       DAOOYB010000091        10576 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_6747_length_10576_cov_9.828153, whole genome shotgun sequence.
ACCESSION   DAOOYB010000091 DAOOYB010000000
VERSION     DAOOYB010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10576
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6747_length_10576_cov_9.828153"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="PL056_06880"
     CDS             <1..273
                     /locus_tag="PL056_06880"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPM46775.1"
                     /translation="ENVIERAVVLSTSDKITQDLFPSELSDIKEEKPQSAKLSSVKKL
                     TQISFIENALKENGFNVPLTAKALGVHKTTLYRIIKKYGIQVESRK"
     gene            358..1509
                     /locus_tag="PL056_06885"
     CDS             358..1509
                     /locus_tag="PL056_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46776.1"
                     /translation="MKRILLVVIFMLMFPLFISASDEDKDVFYFFYSYKCPHCVEAHP
                     FIDKLKKEYPDIIFKDLEVMKVLENRELFKKMIGKLGIQGGGVPTFIFNGKFVVGFQS
                     GHHDKLIRQMIDMNMSDHQKENCQSEDCKECRENMIDVPILGKIDAKMVSLPSFTFII
                     GLLDGINPCAMWVLMFLLTLLVNAKSRKKLIMVGTVFVVSSAVVYFLFMTAWLNIFTF
                     MGVSQYITIALGILALLMGLINIKEFFFFKKGVSLMIPEKAKPKLFEKMRKIMNNSSF
                     AVSLFGTIALAFFVNLIELGCTIGLPAIYTRVLSVQQISTSVKYFYMALYNAYYVIPL
                     AVIVALFVFTMGKYRFEEKHAKVLKLISGILMITLGAILVFKPDLLVFA"
     gene            complement(1513..2220)
                     /locus_tag="PL056_06890"
     CDS             complement(1513..2220)
                     /locus_tag="PL056_06890"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012475892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HPM46777.1"
                     /translation="MSEKKSIKYGEREIFYNLQYLERKTMTIAVHPDGTVIVKAPIQT
                     PFDKIEARLKKRSKWIAKHLDYFINLKPKVTPRQFVSGETHLYLGRRYKLKIIIDNKE
                     KVVATRGNIVVYSKFRDSDRIKNLVEKWYKEKADEHFTKSLNKCFEKFNKYNLRKPAL
                     RIRRMKTRWGSYSTKGTVTLNLELIKAPSECIDYVVTHELCHMLHKNHGNGFYKLLSK
                     MMPEWEKTKSKLERSSS"
     gene            complement(2217..5405)
                     /locus_tag="PL056_06895"
     CDS             complement(2217..5405)
                     /locus_tag="PL056_06895"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015054149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019812 - type I site-specific
                     deoxyribonuclease complex [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HPM46778.1"
                     /translation="MSSFNINEKHISQIPALQLLVNMGYEFISPKEALKERQDKLSNV
                     ILENVLRKKLKSFNRINHKGGEYLFSEENIQSAIQKLKNIKYDGLLQTNEKIYDLLTL
                     GTALEQTVEGDSRSFTLKYIDWRNPERNDFHVTAEFSVERSRSSETARPDIVLFVNGI
                     PFCVIECKAPNIKIDEAVSQSIRNQSDEYIPKLFTYSQMVLGINKNFHKYATPGAGEK
                     FWSVWKEKFSKEDTEIINEKINSELPEETERKICHALQIFKFPETGQRSVTNQDETLF
                     SLCRPKRLLEISQKFTIFDGRDKKIARYQQYFVIKSTLSRIKEKNSDGIRKGGIIWHT
                     QGSGKSLTMVMLTRNLALDDEVKDPRIVLVTDRDDLDKQLGNTFASCNLDPSRATSGR
                     NLLELITEKKSGIITTLIHKFDKALNLKKVQDESSDIFVLVDESHRTQFGSLSARMKQ
                     MFPNACYIGFTGTPLLKKEKSSFAKFGGLIDPHYSINQAVEDGAVVPLLYEGRLVEIE
                     QNKTAIDLWFERHTQGLTEKQKADLKRKYARAEMLNKAERVIYMRAFDICEHFRANWK
                     GTGFKAQLVAPDKASAIKYDEFIKEIGDISCDVVISPPDQREGYEEVDKEPKEEVIKF
                     WNRMMKRYGSEDEYTKQIINQFKNGDEPEILIVVSKLLTGFDAPKNIVLYLCKTLREH
                     NLLQAIARVNRVHENKEFGYIVDYENILGELDKALTMYNEFEGFDETDIEGTLSSISN
                     EIAKLPQIHSDLWDIFKTVKNSYDEEDYERLLEDVSLRDKFYQRLTEYGKSLSNALSS
                     EKFLMETDDRKLEMYKKDFRKFYLLREAVKIRYAESIDYRDYEPKIKKLLDTHIQANE
                     VVQLNEPANIFDSSSFGFIKDAQGVYTGSKSVASQADTIAFAAKKTTSEKMDEDPAFY
                     QNLSKLIQDVIDDFRAGKISDLEYLKTAKELNRKVIGKIHDDIPEKLNGKEDSIAYFG
                     SIKSVFDQNELIHAGNDIIADIAISISGIIDSNKKINFWDDEDAQNKVIDEIDDYLYD
                     EVKNKRNIFLKTEIMDDIINKIMRITRSRDGK"
     gene            complement(5402..6079)
                     /locus_tag="PL056_06900"
     CDS             complement(5402..6079)
                     /locus_tag="PL056_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006366787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle suffix domain-containing
                     protein"
                     /protein_id="HPM46779.1"
                     /translation="MSDLIPKHGGYRKLKSFQISQLVYDVTVRFCELYIDKYSRTRDQ
                     MVQAARSGVQNIAEGSQASGTSKKTELKLTNVARASIEELKLDYEDFLRQKGLEQLKY
                     DHPALVRFKEKQCKTLKEVQEWVKEEREIAKHGRARTEEKNTDRHGQTQTKNNNINSV
                     PVSDSQCQSVSFSSSFLVANAALSLLNLACHLLDRQIEKLAKDFENEGGFTERLYKVR
                     TENRRNK"
     gene            complement(6072..7178)
                     /locus_tag="PL056_06905"
     CDS             complement(6072..7178)
                     /locus_tag="PL056_06905"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HPM46780.1"
                     /translation="MKIPEGWKEKPLEKICDLQAGKFISATEIKETNDGTSYPCYGGN
                     GLRGYVDRYSHDGIFPLIGRQGALCGNINKASGKFYATEHAVVATPCSNIEVNWLYFQ
                     LLFSDLNKYATGAAQPGLSVNNIKKISVPLPPLPEQKAIADILSTWDEAIEKIEKLIK
                     EKEKRFKATVQKLITNNCTTWNHLKPEKIFNSVTEKNFPDEELLSVTQDRGVIPRSML
                     ESRVMSPDGTTETYKLIKKGDFVISLRSFQGGIEYSEYRGIISPAYTVLRPKIEINKD
                     FYKLFFKTYVFIEKYLNIAVIGIRDGKQISIPDFMTIKIPFPPLPEQKRIAETLNTAQ
                     DEINILKKLSEKYKKQKQGLMQKLLTGEWRVRNV"
     gene            complement(7364..8206)
                     /locus_tag="PL056_06910"
     CDS             complement(7364..8206)
                     /locus_tag="PL056_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012475899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HPM46781.1"
                     /translation="MSSSKEIVVLTETVRVYQIHKDDYISLTDIAKHKNADDPRFVIQ
                     NWMRTRFTVEFLGIWEQLNNPDFNRVEFEAVKNEAGSNAFVMTPAKWVALTGAIGIIS
                     NSGRYGGTYAHKDIAFEFASWVSVEFKLYLIKEFQRLKDEELKLLGWDIRRNLTKINY
                     RIHTNAIREHLIPVKLSKEQINLVYASEADILNMALFGKTAKQWRDENPDEKGNIRDF
                     ANVSQLVCLANLENLNAHFIKEGLGQPERLKKLNQTAIEQMRLLIEDRTMKMIEKKSQ
                     DDEK"
     gene            complement(8264..9832)
                     /locus_tag="PL056_06915"
     CDS             complement(8264..9832)
                     /locus_tag="PL056_06915"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012475900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HPM46782.1"
                     /translation="MSTKIEQKDINSAAWAACDTFRGVVDPAQYKDYILVMLFLKYIS
                     DVWNDHYEEYRKQYGDDDERIRRKLERERFVLPEVKIKETNEKTGIDTIFDRFPANFY
                     SLYERRSNDNIGELINIVLDNIEESNKSKLEGVFRNIDFNSEANLGKTKDRNRRLKAL
                     LEDFNKPQLDMKPSRVSEDVIGNTYIYLIERFASDSGKKAGEFFTPLKVTELVAKLAM
                     PKTGDRICDPACGSGGLLVQAAKIINSRDFALYGQESNGSTWALCRMNMFLHSFDSAR
                     IEWCDTLNSPMLVENDRLMKFNCVVANPPFSLDKWGAENAENDRFNRFWRGVPPKSKG
                     DWAFITHMIETALEKEGRVSVVVPHGVLFRGAAEGRIRQKLIEENLLDAVIGLPGNLF
                     PTTNIPVAVLVFDKSRERGGKNDSRKDVIFIDASRDFIQGKNQNTLSEEHLEKIVKTY
                     KNREVIEKYSYLAQFEEIKENDFNLNIPRYVDTFEEEEEIDIDSVQLEIDQLEKELAQ
                     VRAKMAEKLKEIEK"
     gene            complement(9835..10404)
                     /locus_tag="PL056_06920"
     CDS             complement(9835..10404)
                     /locus_tag="PL056_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012475902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HPM46783.1"
                     /translation="MLKNLADIQTGYSFRGRLEYSDSGNVRVIQMKDLTSDNIVDCHE
                     LIKIDMEVLKDHHLAKKGDLVFRSRGINCTASILLEEIENTVVSAPLLKIRAIDRNKV
                     FPEYLKWFINQPESQKFLQSRLSGTHGGMIRASELAELPVSVPDLKTQMLIVEISELS
                     EKEHRILSQISELKKDYISKLLINSSKGE"
BASE COUNT         2817 a         2090 c         1849 g         3820 t
ORIGIN      
        1 gaaaatgtta tcgaaagagc tgttgtttta agcacttcag ataagataac tcaagatctt
       61 tttccgtcag aactttctga tattaaagag gaaaaaccac aatctgcaaa actttcatcg
      121 gtaaaaaaac tgacacagat aagttttata gagaatgcgt tgaaagagaa cggtttcaat
      181 gtcccactta ctgccaaagc gctaggtgtg cataaaacga cactttaccg gatcataaaa
      241 aaatacggaa ttcaggtgga aagtagaaag taattgcaat ttctgaggaa taaattatac
      301 cttcgtggtg ttcttttatt tgttttttat ctttttcttg aaaaaggagt tttaaatatg
      361 aaaagaattc tgcttgttgt aatttttatg ttaatgtttc cgctttttat cagtgcatca
      421 gacgaagata aagatgtttt ctattttttt tattcttaca agtgccctca ctgtgtcgag
      481 gcacatcctt ttattgataa gcttaaaaaa gaatatcctg acataatctt taaagatctt
      541 gaagtgatga aagttcttga gaacagagaa ctttttaaga aaatgattgg aaaactcggg
      601 attcagggcg gcggagttcc aacatttatt tttaacggta aatttgtagt aggttttcaa
      661 tccgggcacc atgataagct cataagacag atgatagata tgaacatgtc cgatcatcaa
      721 aaggagaact gccagagtga agactgcaaa gagtgcaggg agaacatgat cgatgttccc
      781 attttaggta aaattgatgc aaaaatggtc tctttgccga gctttacttt cataatcgga
      841 cttcttgacg gcataaatcc ctgtgcaatg tgggttctga tgttccttct cacccttctt
      901 gtgaacgcaa aaagcagaaa aaaactgata atggtcggaa cggttttcgt tgtttcatcc
      961 gctgtggtct attttctgtt catgaccgca tggctcaaca ttttcacttt catgggggtt
     1021 agtcagtata ttacgatagc acttggaatt cttgcgcttt taatgggact tatcaacata
     1081 aaggaattct tcttctttaa aaaaggggtg agtttgatga ttcctgaaaa agcaaaaccc
     1141 aagctgtttg agaaaatgag aaagatcatg aataacagca gttttgcagt ctctcttttc
     1201 ggcacaatcg cacttgcatt tttcgttaat ctcattgaac ttggatgcac catcggactt
     1261 ccggcaattt atacgagagt tctttcagtt cagcagataa gcacatcggt aaaatatttc
     1321 tacatggctc tctataacgc ttattatgtc attcctcttg cagtaattgt cgctcttttc
     1381 gttttcacga tgggcaaata cagatttgag gaaaaacacg caaaagtgct taaactgata
     1441 agcggaatat tgatgataac acttggagcg atacttgttt tcaaacccga tcttcttgtt
     1501 tttgcttagg agttaagagc ttgatctttc aagctttgac ttggtttttt cccattcagg
     1561 catcattttt gaaagaagtt tgtaaaaccc gtttccgtgg tttttatgca acatgtgaca
     1621 aagttcgtga gtcacaacat aatcaatgca ttccgatggc gctttaatga gttcaagatt
     1681 taatgtaacg gttccttttg ttgaataact tccccatctt gttttcattc ttctgattct
     1741 taatgccggt tttctcaaat tgtatttatt aaatttttca aagcatttat ttaagctttt
     1801 cgtaaaatgt tcatcggctt tttctttata ccatttttcg acaagatttt ttattctgtc
     1861 tgaatctctg aatttgctgt aaacaacaat atttcctctt gttgcgacaa ctttttcttt
     1921 attatcaata attattttaa gcttatatct tctcccaaga taaaggtgag tttctccgct
     1981 tacgaactgt cttggtgtaa ctttaggttt taaatttata aaataatcca agtgcttagc
     2041 tatccacttt gatctttttt tcaaacgggc ttcgatttta tcaaaaggtg tctggattgg
     2101 tgctttaaca atcactgttc catcaggatg gactgcgatt gtcattgttt tcctttctaa
     2161 atactggaga ttgtaaaata tttcccgttc accatattta atggatttct tttcactcat
     2221 tttccgtctc tgcttcttgt tatgcgcatg attttattaa tgatatcatc cattatttca
     2281 gttttaagaa atatgttcct tttgtttttc acttcatcat ataaatagtc atcaatttca
     2341 tcaattacct tgttctgggc atcttcatca tcccagaaat taattttctt atttgaatca
     2401 attattcctg atattgagat tgcgatgtct gcaatgatgt cgtttccggc atgaatcagt
     2461 tcattttgat caaaaactga tttaattgaa ccgaaatatg caatagaatc ttcttttccg
     2521 ttaagttttt caggaatgtc gtcatgaatt tttccaatta cttttctgtt tagttctttc
     2581 gctgttttca agtattccag atctgatatt tttccagctc tgaaatcgtc aataacatcc
     2641 tgaattaatt ttgataaatt ctgatagaat gccggatcct catccatttt ttcacttgtt
     2701 gtcttttttg ccgcaaaagc aatagtgtca gcttgtgatg caacggattt tgatcctgtg
     2761 taaactccct gagcgtcttt gataaaaccg aaagatgagc tgtcaaaaat gtttgccggc
     2821 tcattaagtt gaaccacttc atttgcctga atatgagtgt cgagaagctt ttttattttc
     2881 ggttcataat cgcggtaatc aattgattct gcgtatctta tttttaccgc ttcccgcaag
     2941 agatagaatt ttctgaaatc ttttttatac atttcaagtt ttctgtcatc tgtctccatg
     3001 agaaactttt cacttgaaag agcatttgat agtgattttc catattctgt aagacgctga
     3061 taaaacttgt ctcttaaact gacatcctca agaagccgtt cgtaatcctc ttcgtcataa
     3121 ctgtttttga cagttttaaa aatatcccac aggtctgagt gaatttgcgg cagttttgca
     3181 atttcattac ttattgatga cagggttcct tcaatgtctg tttcatcaaa accctcgaat
     3241 tcattataca ttgtcagtgc tttatcaagt tcaccgagaa tattttcata atcgacgata
     3301 tatccgaatt ccttgttttc atgtaccctg ttaactcttg cgattgcctg tagaagattg
     3361 tgttccctga gtgttttgca aagataaaga acaatgtttt tcggtgcatc aaatcctgtt
     3421 agaagtttgg aaacaacgat cagaatctcc ggttcatccc cgtttttaaa ctgatttatt
     3481 atctgttttg tatattcatc ttcagaaccg tatcttttca tcatacggtt ccagaatttg
     3541 ataacttcct ctttgggttc tttgtcaacc tcttcgtatc cttctctctg atcaggaggg
     3601 gaaataacaa catcgcaaga tatatcacca atttctttaa taaattcatc atattttatt
     3661 gcggatgctt tatcgggggc aactaattga gctttgaagc ctgtcccttt ccagttagct
     3721 ctgaaatgtt cacaaatatc aaaagctctc atataaatta ctctttcagc tttattgagc
     3781 atttccgctc ttgcatattt ccttttgagg tctgctttct gtttttcagt aagtccctga
     3841 gtgtgtcttt caaaccagag atctattgca gttttgttct gctcgatttc aacaagccgt
     3901 ccttcataaa gcagtggaac gacagctccg tcttcaacgg cctgatttat tgaatagtgc
     3961 ggatcaatca gtccgccgaa cttggcgaaa ctgctttttt cctttttaag aaggggtgtt
     4021 cctgtaaatc cgatatagca ggcattggga aacatttgtt tcattcttgc tgacagtgat
     4081 ccgaactgag ttctgtgact ttcatccaca aggacaaaaa tatctgatga ttcgtcctga
     4141 actttcttta aattcaatgc tttatcaaat ttatggatca gtgtggtgat aattccgctt
     4201 ttcttttctg tgatgagttc aagaagattc cttcccgaag ttgcacggct cggatcaaga
     4261 ttgcacgatg cgaaagtatt tccaagctgc ttgtcgagat catcacggtc tgtgacaaga
     4321 acaattctcg gatctttcac ttcatcatca agagcaaggt ttcttgtgag cattaccatt
     4381 gtgagtgatt tgcctgagcc ttgagtatgc cagattattc ccccttttct aatgccgtca
     4441 gaattttttt ctttgattct tgaaagggtg ctttttatta caaaatactg ctgatatctt
     4501 gcaatctttt tatctctgcc gtcaaaaatc gtgaatttct gtgatatttc aagaagccgc
     4561 ttaggacggc ataaagaaaa gagtgtttca tcctgatttg tgaccgatct ttgcccggtt
     4621 tcaggaaatt tgaatatctg aagagcgtgg cagatttttc tttcagtttc ttccggcaat
     4681 tctgagttaa ttttttcatt tatgatttca gtatcttcct ttgaaaactt ttctttccat
     4741 acagaccaga atttctcacc tgctcccgga gttgcgtatt tatgaaagtt cttatttatt
     4801 cccaaaacca tttgagaata tgtgaacagt ttcggaatat attcatcaga ctgattccgg
     4861 atggattgag aaaccgcttc gtcaatttta atattcggag ctttgcactc aataacgcaa
     4921 aaggggattc cattcacaaa aaggactatg tcaggtcttg cagtttcaga gcttcttgat
     4981 ctttcaacgc tgaattcagc tgtaacatga aaatcgtttc tttcaggatt ccgccagtcg
     5041 atatatttta aagtgaaact tcttgaatct ccctcaacag tctgttcaag agcggttccc
     5101 agagttaaaa ggtcatatat tttttcattt gtctgcaaaa gcccgtcata tttgatgttt
     5161 ttgagtttct gaattgcaga ctgaatgttt tcctcactga aaagatattc tccccctttg
     5221 tgatttatcc tgttaaaact tttaagtttt ttacgcagaa cattttcgag gatcacattt
     5281 gaaagtttat cctgccgttc tttcagagcc tctttcggag aaatgaactc atatcccatg
     5341 tttaccagaa gctgtaaagc aggaatctgg gaaatgtgtt tttcattgat gttgaaactg
     5401 ctcatttatt tctcctgttt tcagttctta ctttgtaaag ccgttccgtg aaaccgccct
     5461 cgttttcaaa atcttttgca agtttttcaa tttgcctgtc gagtaagtga caggcaagat
     5521 ttaaaagtga aagtgccgca tttgcaacaa ggaaagaaga agaaaaactc acagactggc
     5581 actgactgtc actgactggc acggaattaa tattattatt ttttgtctgt gtttgtccgt
     5641 gtctgtccgt gttcttttct tctgtccgtg ctcgtccgtg ttttgcaatt tccctttcct
     5701 ctttcaccca ttcctgcact tctttcagtg ttttgcactg tttttccttg aatctgacaa
     5761 gtgccgggtg atcatatttg agttgttcaa gtcctttctg acgaagaaaa tcttcatagt
     5821 cgagtttcag ttcttcaatg cttgcacggg cgacatttgt cagtttgagt tctgtttttt
     5881 tggatgttcc ggatgcttgg gaaccttctg caatgttctg gactccgctt cttgcggctt
     5941 gcaccatctg gtcacgggtt cgggagtatt tgtcaatgta aagctcgcaa aaacggacag
     6001 ttacatcata aaccagttgt gaaatctgga aacttttcag tttgcggtaa ccgccgtgtt
     6061 ttgggatcag atcagacatt tctcactctc cattctccag tcagcaattt ctgcatgagt
     6121 ccctgttttt gttttttata tttttctgat agttttttca ggatgttaat ttcatcttga
     6181 gcggtgttga gtgtttcagc gattcgtttt tgttcgggga gtggcgggaa aggaatttta
     6241 attgtcataa aatcgggaat gctgatttgt tttccgtctc ttattccaat gaccgctata
     6301 tttaaatatt tttcaataaa aacataggtt ttaaaaaaca gtttatagaa atctttattt
     6361 atttcgatct tgggtcgcaa caccgtatat gctgggctga tgattcctct atattctgaa
     6421 tattcaatcc ctccttgaaa agaacggaga cttattacaa aatcaccttt tttaataagt
     6481 ttatatgttt ctgttgttcc gtctggagac ataacacgac tttcaagcat tgaacgggga
     6541 ataacaccac gatcttgtgt tacagaaagc aattcttcat cggggaaatt cttttctgtc
     6601 acagaattaa atattttttc aggctttaaa tgattccatg ttgtgcaatt attcgttatt
     6661 agtttttgaa cagttgcctt aaatctcttc tccttttcct ttatcaattt ctctattttt
     6721 tcaatcgctt catcccatgt tgaaagaata tctgcaattg ctttctgttc tgggagggga
     6781 ggtaaaggaa ctgaaatttt tttaatgtta tttacagaaa gtcctggttg agctgcgcca
     6841 gttgcatatt tattcaaatc tgagaaaaga agctgaaaat acaaccagtt gacttcaatg
     6901 tttgagcatg gtgtagctac aacagcatgt tctgttgcat aaaattttcc acttgctttg
     6961 ttaatgtttc cacacaaagc accctgtctt cctattaaag gaaagattcc atcatgtgaa
     7021 tacctatcaa cataaccacg cagtccattt cccccgtaac aaggatacga tgttccgtcg
     7081 ttggtttctt ttatttcagt ggcggaaata aacttccctg cttgtaagtc acaaatcttt
     7141 tcaagaggtt tttctttcca tccttccggt attttcattt cccctccggt ttatttatgc
     7201 agaccatttc gaggaagtcc tcgatatggt gtttttcctg ttttgaccat tttgatctct
     7261 tcctcaatat ggttttttat catttcgttc ttttccacga gatgataaat cgaacataat
     7321 ttttttaaag gggtcgaaat cgacccggtt aagattttta cttttatttt tcatcatcct
     7381 gactcttctt ttcaatcatt ttcattgttc tgtcttcaat cagtaatctc atttgttcaa
     7441 ttgcagtctg attcaatttt ttgagtcgtt ccggttgtcc caatccttct ttaataaaat
     7501 gggcattcag attctcaaga ttggcaagac acaccagctg tgatacattg gcaaaatcac
     7561 ggatatttcc tttttcgtcc ggattttcat cccgccattg ctttgctgtt tttccaaaaa
     7621 gtgccatgtt taatatgtct gcttctgatg cataaaccaa gttgatttgc tctttactta
     7681 atttcacggg gatcaagtgt tctctaatag cattggtatg aatccgatag ttaatttttg
     7741 tcaaattccg ccgaatatcc cagccgagca gcttgagttc ttcatctttc aatcgctgaa
     7801 attcttttat tagataaagt ttgaattcta ccgataccca tgatgcaaat tcaaaagcaa
     7861 tgtctttgtg ggcataggtg ccaccatagc gaccagaatt ggagataata ccgatagcac
     7921 ctgttaaagc gacccacttg gccggagtca ttacaaatgc gttacttccg gcttcatttt
     7981 taaccgcctc gaattcgaca cggttaaaat cagggttgtt caattgttcc caaataccca
     8041 gaaattctac cgtaaagcgg gttctcatcc agttctgaat gacaaagcga ggatcatcag
     8101 cgtttttatg tttagcgata tctgtcagag agatataatc atctttatgt atttggtaaa
     8161 cgcgcactgt ttcggtaaga actactattt ctttacttga tgacatgatt aattctcctt
     8221 tctaattccg gtaatattcg tgtacatcag tgtccatttt tgattacttc tcaatctcct
     8281 tcaacttttc agccattttt gctctcactt gagcaagttc cttttcgagc tggtctattt
     8341 cgagttgaac tgaatcaatg tctatttcct cttcttcttc aaaggtatct acatatcggg
     8401 gaatgttgag gttgaaatcg ttttccttta tttcttcaaa ttgtgcgaga tatgaatatt
     8461 tttcgatgac ctctctgttt ttatatgttt ttactatctt ctcaaggtgt tcttctgaaa
     8521 gcgtgttctg attctttccc tgaatgaaat ccctgcttgc atcaataaaa ataacatcct
     8581 ttcttgaatc atttttcccg cctctttcac gacttttgtc aaaaacgaga accgccacag
     8641 gaatattggt tgtcggaaac agatttcccg gcagtccgat gactgcatca agaagatttt
     8701 cttcaataag tttctgtctg attctccctt cagcagctcc acgaaataaa actccgtgag
     8761 gaacaacaac agaaactctt ccttcctttt caagtgctgt ttcaatcatg tgggtaatga
     8821 atgcccagtc acctttgctt tttggtggaa cgcctctcca gaacctgtta aaccggtcgt
     8881 tttctgcatt ttctgcgccc catttatcga gtgaaaacgg aggattggca acaacacagt
     8941 taaatttcat caatctgtca ttttccacaa gcattggact gttgagtgtg tcacaccact
     9001 caattctcgc actgtcgaaa ctgtgaagga acatattcat tctgcacaaa gcccatgtgc
     9061 tgccgtttga ttcctgtccg taaagtgcaa agtctctgct gttaattatt tttgccgcct
     9121 gaacaagcaa tccgcccgaa ccgcatgccg gatcacaaat tctgtctcct gttttaggca
     9181 ttgcaagttt tgctacaagc tcagtgactt tcaaaggagt gaagaattct cctgcttttt
     9241 tgcctgaatc agaagcaaac cgctcaatga ggtaaatata tgtatttcct ataacatctt
     9301 ctgaaactct gctcggtttc atgtcaagct gaggtttgtt aaaatcttca agaagtgctt
     9361 taagacgacg gtttctgtct ttggtttttc cgagatttgc ttcactattg aagtcaatat
     9421 ttctgaaaac cccttcaagt tttgatttgt tggattcttc aatgttatca agaactatgt
     9481 tgataagttc gccgatatta tcatttgatc ttctttcata aagactgtaa aaattagcag
     9541 gaaacctgtc aaaaattgta tcaattccgg ttttttcatt tgtttcttta attttcacct
     9601 caggaagaac aaaccgctct ctttcaagct ttcttcttat tctttcgtca tcatctccat
     9661 actgctttct gtattcttca tagtgatcgt tccatacatc tgatatatat ttaaggaata
     9721 acatcacaag aatgtaatct ttgtactgag ccggatctac aactccacgg aaagtgtcac
     9781 acgccgccca cgccgcactg tttatatcct tctgctcgat ttttgtactc atccttattc
     9841 tccttttgat gaatttatta ataattttga gatgtaatct tttttgagtt ctgaaatttg
     9901 tgacaatatc cggtgttctt tttcagataa ttctgatatt tcaactatca gcatctgcgt
     9961 tttcaagtcg ggaacagaaa caggaagttc ggcaagctcg gaagccctta tcattcctcc
    10021 atgagtgccc gacaaccttg attgcaaaaa tttctgagat tccggctgat tgatgaacca
    10081 tttaaggtat tcaggaaaaa ctttattgcg atctatcgcc ctgattttta gaagaggagc
    10141 agaaaccaca gtgttctcaa tctcttctaa taaaattgat gctgtacagt taattcctct
    10201 tgatctgaaa acaagatctc cctttttcgc aagatgatga tctttgagaa cttccatatc
    10261 tatttttatc agttcgtgac agtcaacaat gttatcagaa gtcagatctt tcatctgaat
    10321 aacccttaca tttcccgaat cagaatattc aagacgcccc ctgaaagagt atcctgtctg
    10381 aatatctgcc agatttttca gcatcgtttt caaaaaaacc tcctcaatgc aaaaacagca
    10441 ttacagagga aattatacgc aaaaaatttg atttgtcaag aaaattttgc agaaaagtcg
    10501 ttactgacaa gaaagtcgtg tttattcggc aagcttctta aagatctttt cacctatctt
    10561 ttcaaattcg attccg
//
LOCUS       DAOOYB010000092        10519 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_6811_length_10519_cov_10.264430, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000092 DAOOYB010000000
VERSION     DAOOYB010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10519
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6811_length_10519_cov_10.264430"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..1674)
                     /locus_tag="PL056_06925"
     CDS             complement(145..1674)
                     /locus_tag="PL056_06925"
                     /inference="COORDINATES: protein motif:HMM:NF014133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003712 - transcription coregulator
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAP domain-containing protein"
                     /protein_id="HPM46784.1"
                     /translation="MKINECIEKMSKRSLVKVLKAVLKNDVPKNADDPQLKKLVEDNV
                     SLFSGHERIEKELNFNSYKRNERILVNEILMIVLESDDSIISEKELWEKVQEYEKRIV
                     EKAKNNNISRVIGQNHYEIYKTILEVALEDDNMSPDEYNIVKKLRKKLKINSYQSRII
                     EAELGKFPGAENKLHSVSDFELMLKILQEKGILFYFKEKDECYYAVIPSEIENSVKSI
                     MEFELGIGAHNKMLDKLKMSQLIEILKSFGLASSGKKEEIISRIIEAGFYPSEELDVL
                     SNDELAEICRDLPGVNVTGTKPERSRRIIAYFNNLKTKIETKSEDERAKYYEVFEKLA
                     TRDELYLMAQNIIKDEKMIGSCFEKATRYLFEKKLGHDVVPRKGVVSGDGEVRLSDNE
                     VLLWDNKSKRKDYKFCKDHQRQFKDYIDSSEQIVRVFLVIVPFVDKNDEMEVLMAARD
                     IKNSTKNRTEIAIITAEDLKYIADKWRNFSTKEKFDLSIFNYTGVLKKNYLTESMKRM
                     K"
     gene            complement(1820..2161)
                     /locus_tag="PL056_06930"
     CDS             complement(1820..2161)
                     /locus_tag="PL056_06930"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HPM46785.1"
                     /translation="MPHKNDLPYLLHIKDALNDISRFTQGMDFTTFYEDDLVSSAVIR
                     KLEVMGEASNKVSAETKEKFPQIPWRMMVDMRNILIHHYFGVDLSAVWDTIKNDLPEL
                     KQKIEEVVENF"
     gene            complement(2151..2450)
                     /locus_tag="PL056_06935"
     CDS             complement(2151..2450)
                     /locus_tag="PL056_06935"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HPM46786.1"
                     /translation="MVNSKKDILKNDELRKFCKENGIVFIALFGSFVRGEENSGSDID
                     LVVRFQNTRSLFQIIETRNRLTEILGREVDLVTENSLSPYLKEKIMNEMEAIYAA"
     gene            2611..4449
                     /locus_tag="PL056_06940"
     CDS             2611..4449
                     /locus_tag="PL056_06940"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HPM46787.1"
                     /translation="MKKFLWLFIGVLMLTGCPSKKVEVKEAPVEEIKEKTIVDYINIE
                     GSHSGYIFKDGRKLFIARKENAAQLYLESSCGKDVKQLTFQEDAVEGYVVSPEEKQIL
                     YIISKGGNEQYDFHVYNLEKGNSEGLLVDDAVRFESPRWLNENELMFTSNEANGKDFY
                     IYHLDIPSKKKTLLVEKPGYNHITDAISKDEFLFYTFLGNNKTAPYHFKNGKPKKIKG
                     AQKERNFVPLAFFEDGILMETNEESDVEYLEIWKDGVKKPLFKDIWGVESVVVDKISR
                     KHAAFCTNEDGYSSCSYYSDGKVEQIPLKKSVVFLTRIDGERIIYNMMNPDRISSPAA
                     FHFSRKESVSFGYTFDNGVDISGFAAPELKKVKGADGVEIPYFLYTPKNGTPPYNTIV
                     YFHGGPEGQFRPYFIATYQYYLKKGFAIVAPNVRGSSGYGQVFMDMDNYKLRMNSVSD
                     GKTVTDELVRLGISKPEKFIAMGGSYGGFMVVASMAKFSDDYHCGINSVGVVDFVNFL
                     ENTAGYRRHLREVEYGPLTDREFLMSISPTNMVDTIKGELFVFHGANDPRVPVSDAYI
                     LMEKMKKAGKKYQHHIFEDEGHGFKKRENRVIYNSKSAEFIEKCGE"
     gene            complement(4450..5532)
                     /locus_tag="PL056_06945"
     CDS             complement(4450..5532)
                     /locus_tag="PL056_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46788.1"
                     /translation="MKKITFITIVCAVFIFAACENSTNTAKDNEPTVDEEEVTDEQPD
                     ETVDETVDEVVDENEVPDEFDPSNPGYTVEFFFADMNMMGTPTAIIGGYVMKTAREDL
                     DEEYEGPTYMIDTCVLGEEPPREPECQSKDDCAPEQECVPDYDDSGNPKPGTEHCATP
                     GRESLDVGPIKIKGFASGEQTFLYEPNDQIYKLNGEGDGSVDSSLITYNQKYELYAED
                     PTPEDLTPFNGEFFLGNKLELTSHTVVSDGGGGFPFIELDMTQPLTFKWTGNEGEGYL
                     EMTITAMLDMSNSVSVSCTVADDGEFTIPDEYASQLQFGTGMMAQMGSIFSITRKSKS
                     PFSGDTISYGKFGSDQILFTNVRPKQ"
     gene            5686..6975
                     /locus_tag="PL056_06950"
     CDS             5686..6975
                     /locus_tag="PL056_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46789.1"
                     /translation="MKKFLKTFLFLAVFFSVIHADDMFPNIAVVNTNDDGLIGAIEKS
                     AGQRFYMNHPDSFDFLFIFTTFTPSINMQQGIPIQYTVEGINRDAGIMNPYGTPVDWG
                     SSGKLLGAARMCNIDQYPENPDVQISTGILNPYSGMSTVELLAHEWSHYWLAAMDFKK
                     EGMTEPHTGLRGWEDGANQHWSGDFMSGPSVMYGGDITDNGDGTVTYQFDNPKKYGPL
                     DLYVMGFIPPQEVGELFFMCSSADINQCKEGNPAVPANKTSPDSVKSGLTKHIITIDD
                     IIRAMGERIPSSAEAPKHFNVAFILVSKDGFSPFPQQLQKLETLRVRFQEWFPWATDG
                     KGTICTELDGDCTNGEEPDDDETVDENNPLVDETVEIPDETTEIPDESKDEEIQDDNE
                     TIDNNEPVNDNILETADDQNEEFIEDETAGCGCTVVF"
     gene            7030..8631
                     /gene="rseP"
                     /locus_tag="PL056_06955"
     CDS             7030..8631
                     /gene="rseP"
                     /locus_tag="PL056_06955"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HPM46790.1"
                     /translation="MYMYLYAVLLLGVVVFIHELGHFLFARLFGVRVLAFSLGFGKPL
                     IKWKRGDTEYRISLIPLGGYVKMLGESPDDEKALENNEYAYAYSHKLWWQKVIIAFAG
                     PLFNIIMAFVVFMFVSFFEYSAPASVVEYVEPGGPACEAGMEEGDRITEINGRPVHVW
                     EDIQISISSSLKEKKCAETEISVVRPDGSKATLSVIPKTGTYSDVFNEEIERCEIGIA
                     RLPRDASIALLSEFKGLQNGDIIISIDGAPVSRFYEVEKRLKGASFKTIKLKRNGEEY
                     AVSFENEAVPSLVHGGLIVSKVEEGSFSSGIGLEKGDIINSVNGRTITIPYQFYSIMQ
                     KLKEGDDVTIGLVRNYEKKEIIFKASFEEKENAMTGLTDRNIKWGANFAFNYDVPEIY
                     AKRAEPFKFTFKYASEQTFGMISMTLKGFWYLVTGKMSAKSLGGPIMIFDISKKAAEA
                     GLKHFLFILAVISINLGIINLFPVPILDGGYIVMYTIEGIVRKQIPVSIKEKMLTAGL
                     VMLLLLMAFAIFNDFSRYISIFTRV"
     gene            8632..9348
                     /locus_tag="PL056_06960"
     CDS             8632..9348
                     /locus_tag="PL056_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46791.1"
                     /translation="MKNLLVFDGSTKNILFGGTLENSCIFHCINFERSKMSCRLPDFF
                     LNLKPDIRFETLPEVIIGNGPGPFTGLKTANSFFLGFIYSLGLKKVRLVSSFKIISAL
                     SPAEGNLNRLVVIPFNKGEYFLGLLDKENNLLVKDVFIKESSENIKDIFKDYLNSKIE
                     LILGSECGATLIEALRSHFDINSTHQEEYHFDHQRQLSLLGEELLDITKDPFILNHVI
                     NPADIDKNCNIYVSNNFEEG"
     gene            9349..9564
                     /locus_tag="PL056_06965"
     CDS             9349..9564
                     /locus_tag="PL056_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46792.1"
                     /translation="MKAENLSTEVILEKISKLKEEHSRYDELSNELAAKKTFTPHDEV
                     ELNTLRKKKLMKKDMIAYYEGLLKNTK"
     gene            9659..>10519
                     /locus_tag="PL056_06970"
     CDS             9659..>10519
                     /locus_tag="PL056_06970"
                     /inference="COORDINATES: protein motif:HMM:NF039815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46793.1"
                     /translation="MKKLVSAFLFVSFFVIFSACGSDDEEEKKQCIADSDCPFPYKCD
                     LEIFRCKHPETGELLDDGNESGDSGNSGNSGDSGNTGNTGNSGNSSNDPNKDDPCFDR
                     NADADGDGVPNNVECPFFPCSDSDGDGKPNCLDLDSDDDGISDADEKTNGTNPYNSDT
                     DSDGLTDYQESQYGTNPKMKDSDSDGFDDLAEIAYGTDPNNNADKIPEDVFYVVLPYK
                     PGFETKKQLDFSTDIQQADIAILVDLSGSMSGEHSNLKQGINNVIITGVKAQIPNTGF
                     GLVKFGTLQDQ"
BASE COUNT         3240 a         2065 c         2062 g         3152 t
ORIGIN      
        1 tgaattaaat atgcaagaaa tcaaatacga ttatttaagt caacagaaaa cagaaaaaag
       61 cggaattgat taagggggtt ggtaagtttt atgcgacaaa gatgtgtgtg aaaatcgcag
      121 agcaacccgt atggtcagcg gacattattt cattcttttc atagattctg taagataatt
      181 ctttttcagc acaccggtat agttaaaaat acttaaatca aatttctctt tcgtggagaa
      241 gtttctccat ttatctgcaa tatattttaa atcctctgct gtaataatag ctatttccgt
      301 tctgttcttt gtgctgtttt taatatctct ggccgccatc aaaacttcca tttcatcgtt
      361 tttatcaaca aatggcacta ttaccagaaa aactcgaacg atttgttcag aagagtctat
      421 ataatctttg aattgccttt gatgatcttt acaaaattta tagtccttcc ttttgctttt
      481 attatcccac aacaacactt cattatcact taaacgcact tcaccatctc ctgaaacaac
      541 gccttttcta ggcacaacat catgtcctag ttttttctca aaaagatacc ttgtggcttt
      601 ttcaaaacag cttccaatca ttttctcatc tttaattata ttttgagcca ttaaatataa
      661 ttcatctctt gttgcaagct tttcaaagac ctcataatat tttgcccttt catcctcgga
      721 tttggtttca attttagttt taagattgtt aaaatacgca attattcttc tgcttctttc
      781 aggttttgtt cctgtcacat taactccagg aagatcacga catatttcag caagttcatc
      841 attacttaaa acatctaact cttcagaagg ataaaatcct gcctcaatta ttctagaaat
      901 gatttcttct tttttccccg aagaagcaag accgaatgat ttgagtattt ctatcagctg
      961 gctcatctta agtttatcaa gcattttatt atgtgcacca attcccaact cgaattccat
     1021 aatacttttt acagaatttt ctatttcaga agggataaca gcatagtaac actcatcctt
     1081 ctctttaaaa taaaacagaa tccctttttc ttgaaggatt ttaagcatta actcaaaatc
     1141 agaaacagaa tgaagtttgt tttcagcccc tggaaatttg ccaagctcag cttcaataat
     1201 tctgctctga taactattta tctttaattt ttttctcaac ttctttacaa tattgtattc
     1261 atctggagac atattatcat cctctagtgc cacttcaaga attgttttat aaatttcgta
     1321 gtggttttga ccaataactc ttgaaatatt gttgttctta gctttttcaa caatcctctt
     1381 ctcatattcc tgtactttct cccaaagctc tttttctgaa ataatcgaat catcactttc
     1441 aagaacaatc atcagtattt catttaccag gattctttcg tttcttttgt atgaattaaa
     1501 attcagttct ttttctattc tttcatgacc agaaaacaga gaaacattgt cttcaaccag
     1561 cttttttaat tgcggatcat ctgcgttttt gggcacatca ttttttaaaa cggctttgag
     1621 tactttaaca agacttcttt tagacatttt ctcaatacat tcgttaattt tcataggaat
     1681 cactccacat cagaaattaa tattttcaac cttaattgat tttatttgac ttgctttata
     1741 caaatccccc tcatttagct tgtctgaaca aaagctcaaa ccaaaaaagt gaaaaattat
     1801 aacaacacaa atgaaaaact caaaaatttt caaccacttc ttcaattttc tgtttgagtt
     1861 caggcagatc attttttatg gtgtcccata ctgcggaaag atcaacaccg aagtagtgat
     1921 gaatgagaat atttcgcata tctaccatca ttcgccaggg tatttgcgga aacttttctt
     1981 ttgtttcagc agaaacttta tttgatgctt cacccatcac ttcaagcttc cttatcacag
     2041 cgcttgacac aagatcatct tcataaaatg tcgtaaaatc catgccttgg gtaaacctcg
     2101 aaatatcatt caatgcatct tttatgtgaa ggagatacgg cagatcattt ttatgcggca
     2161 tatatcgcct ccatttcatt catgattttt tctttaagat atggactcaa tgaattttca
     2221 gttacaagat caacttctct tccaagtatt tcggtcagtc tgttccttgt ttcaattatc
     2281 tgaaaaagac tccttgtgtt ctgaaatctc acaacaagat caatgtcact gccggaattt
     2341 tcctctcctc ttacaaatga accgaaaaga gcaataaaaa caatgccgtt ttccttgcag
     2401 aacttcctta attcgtcgtt ctttaaaata tcttttttgc tgttaaccat tttcaagtac
     2461 ctcaaaaaat tcagtcttta ttttacatgg attatttaaa aaagaaagaa ctggctgtaa
     2521 tatgatcttt tctccaaata attgaaatag tttcaagtaa atgttatttt ggtgcggttt
     2581 gtgttgtaaa ctgttttttc ggagaggaaa atgaaaaagt ttttatggtt atttatcggt
     2641 gttttaatgt tgacgggatg cccttcaaaa aaggttgaag ttaaagaggc cccggttgag
     2701 gaaattaaag aaaaaacgat tgttgactat atcaatattg aaggaagcca ctcgggatat
     2761 attttcaaag atgggcgaaa actttttata gcaaggaagg aaaatgcggc tcagctttat
     2821 cttgaatcaa gttgcggtaa agatgtgaaa cagcttactt ttcaggaaga cgctgttgaa
     2881 ggatatgttg taagtcctga ggaaaaacaa atcctttaca tcatttcaaa agggggaaat
     2941 gaacagtacg attttcatgt ttataatctt gaaaagggca atagtgaggg acttcttgtc
     3001 gatgatgcag taaggtttga aagtccgaga tggctgaatg aaaacgaact tatgttcact
     3061 tcaaatgagg caaacggaaa agatttttat atttatcatc tcgatatccc atcaaaaaag
     3121 aaaactcttc ttgtcgaaaa accgggatac aaccatataa ctgacgctat aagcaaagat
     3181 gaattccttt tctacacatt tcttggcaac aacaaaacag ctccttatca tttcaagaac
     3241 gggaagccga aaaagatcaa gggagctcaa aaagagagaa actttgttcc gcttgcattc
     3301 tttgaagacg gaattcttat ggaaacgaac gaagaaagcg atgtggaata tcttgaaata
     3361 tggaaagacg gggtcaaaaa accgctcttt aaagacattt ggggagtaga atcggtcgta
     3421 gttgacaaaa tttcaagaaa acatgccgca ttctgcacaa atgaagacgg ttacagttca
     3481 tgcagttatt attctgacgg taaagttgag cagatacctt taaaaaaatc ggttgtgttt
     3541 ctgacaagga tagatggcga gaggatcatt tacaacatga tgaaccctga cagaataagc
     3601 tctcctgccg cttttcattt cagcagaaag gaatccgttt ccttcggcta cacatttgat
     3661 aacggcgttg atatttccgg ctttgccgct cccgaactta aaaaagttaa aggagctgac
     3721 ggtgttgaaa taccgtattt tctctacact cctaagaacg gaacaccgcc ttataacacg
     3781 atcgtctatt ttcatggcgg accggaaggg cagttcagac cttattttat tgcaacatat
     3841 caatattatt tgaaaaaagg attcgccata gtagctccga atgtaagggg ctcatccggc
     3901 tacggacaag ttttcatgga tatggacaac tataagctga gaatgaactc ggtgagtgac
     3961 ggaaaaacgg tgacagatga acttgtgcgg cttggaatat caaaaccgga aaaattcata
     4021 gcaatgggtg gaagttacgg cggattcatg gttgttgcct cgatggcgaa attcagtgat
     4081 gattaccatt gcggaattaa ttctgtagga gttgttgatt ttgtcaattt ccttgaaaat
     4141 acagccggct acagaagaca tttgagggaa gtcgagtacg ggccgctcac tgacagggag
     4201 tttttaatgt ccatatcacc tacgaatatg gttgacacaa ttaaaggcga gctgtttgtt
     4261 tttcacggag cgaacgatcc gagagttcct gtcagcgacg cttatatcct gatggaaaaa
     4321 atgaaaaagg cgggaaagaa atatcagcat cacatctttg aagatgaggg tcacggcttc
     4381 aaaaaacggg agaacagagt catatacaat tcaaaaagtg ctgaatttat tgaaaaatgc
     4441 ggtgaataac tactgtttcg gccttacatt tgtaaaaagt atctgatcgc tcccgaactt
     4501 tccatatgat atagtatcac cggaaaatgg agatttcgat tttcttgtta tggagaagat
     4561 gcttcccatc tgtgccatca ttcccgttcc gaactgaagc tgggaagcgt attcatcggg
     4621 aattgtaaat tcaccgtcat ctgcaacggt acaactgaca ctgacagaat tactcatatc
     4681 aagcattgcg gtaattgtca tttcaagata tccttcacct tcgttgcctg tccatttgaa
     4741 agtaagcggc tgagtcatgt caagttcaat gaaaggaaat ccgccgccac cgtcagaaac
     4801 aaccgtgtga cttgtgagtt caagcttgtt cccgagaaaa aactctccat tgaaaggggt
     4861 gagatcttca ggggtcggat cttcagcata aagctcatat ttctgattat atgtgataag
     4921 ggaagaatca acacttccgt caccttctcc gttgagctta tagatctgat cattcggctc
     4981 atatagaaaa gtctgctctc cgcttgcaaa accttttatt ttgatgggtc cgacatccag
     5041 cgattccctg ccgggcgttg cacagtgttc agttccaggt ttcggatttc cgctgtcgtc
     5101 ataatcggga acacactcct gttcaggtgc gcaatcatct tttgactgac attccggctc
     5161 tcttggcggc tcttcgccca gaacacaggt gtcaatcata taggtcggac cttcatattc
     5221 ttcatcaaga tcctctcttg ccgttttcat cacataaccg ccgattattg ctgtgggcgt
     5281 tcccatcata ttcatgtcgg caaaaaagaa ttcaaccgta tatcccggat ttgacgggtc
     5341 aaattcatcc ggaacttcat tttcatcaac aacctcatca acagtttcat caacagtttc
     5401 atccggctgc tcatcagtaa cttcctcttc atcaactgtc ggctcgttgt cttttgcggt
     5461 atttgtgctg ttttcacacg cagcaaaaat aaaaaccgca caaacaatcg taataaaagt
     5521 aattttcttc acaacttcct ccttgtttac acacaacaga tgaaaatata gccgaaacgg
     5581 aaaataagtc aaaaacagac aaacctcttt ttagcttgca aaaaaacact gaatctgtaa
     5641 gaatattcaa gttgtgtgga atttggcata aaaagcgagg tcaaaatgaa gaagttttta
     5701 aagacatttc tgtttttagc tgtctttttt tcagtgattc atgctgatga catgttcccg
     5761 aacattgctg ttgtgaacac caatgatgac gggcttatcg gtgcgatcga aaaatcggcc
     5821 ggacaaagat tctacatgaa ccatcccgat tcttttgatt tccttttcat ttttacgaca
     5881 tttacccctt caataaacat gcagcaggga attccaatcc agtataccgt tgagggaata
     5941 aacagggatg caggcataat gaacccttac ggaacgcctg ttgactgggg aagttcagga
     6001 aaacttctgg gtgccgccag aatgtgtaat atcgaccagt atcctgaaaa cccagatgtt
     6061 cagatctcaa caggaatact caacccttat tccggaatgt caacagttga acttctggct
     6121 cacgaatgga gtcactactg gcttgcggca atggatttta aaaaagaggg catgactgag
     6181 ccgcacacag ggcttcgcgg ctgggaagat ggcgcaaacc agcactggag cggagatttt
     6241 atgtcaggac catcggttat gtacggaggc gacatcacag acaacggtga cgggacggtt
     6301 acatatcagt ttgacaatcc gaaaaaatat ggacctcttg acctttatgt gatgggcttt
     6361 attcctcctc aagaagtggg ggaacttttc tttatgtgct catcagcaga tattaatcag
     6421 tgcaaagaag gaaacccggc agttccagcc aacaagacat cacccgattc agttaaatca
     6481 ggacttacaa aacatattat aaccattgac gacatcatcc gggcaatggg tgaaaggatc
     6541 ccttcatcag ccgaggcacc gaaacatttc aatgtggcat ttattcttgt gagcaaagac
     6601 ggtttttctc catttccgca acagcttcag aaactggaaa ctttaagggt ccgttttcag
     6661 gaatggttcc catgggcaac ggacggaaaa gggacaatct gtacagaact tgatggcgac
     6721 tgcacaaacg gagaagagcc ggatgatgat gaaacagttg atgaaaataa tcctctggtt
     6781 gacgaaactg tcgagatccc cgatgaaaca acagaaattc ctgacgaatc aaaagatgaa
     6841 gaaattcaag atgataacga aaccattgac aacaacgaac ctgtcaatga caacatctta
     6901 gaaacagcag acgaccagaa cgaagaattc attgaagatg aaactgccgg atgcggctgt
     6961 actgttgtat tctgatattt tgtgattata ataccccaag ttattgtttt ttagattgga
     7021 tggttgaaaa tgtacatgta tctatacgct gttctgcttc tcggggtagt tgtttttatc
     7081 catgaacttg gtcattttct ctttgcccgg cttttcggag taagggttct tgctttttcg
     7141 ctcggattcg ggaaaccgct gataaaatgg aaaagaggtg acaccgaata caggatttca
     7201 cttatccctc ttggcggcta tgtaaaaatg ctcggagaat cacccgatga cgaaaaagct
     7261 cttgaaaata atgaatatgc ttatgcctat tctcacaaat tgtggtggca gaaagtcatc
     7321 attgcatttg cagggccttt attcaacatc ataatggcgt ttgtcgtgtt tatgtttgtc
     7381 tctttttttg aatattcagc ccctgcatcc gtagtggagt atgttgagcc gggaggacct
     7441 gcttgcgaag ccggaatgga agaaggtgac aggatcacag agatcaacgg gagaccggtt
     7501 catgtctggg aagatatcca gatctcgatc tcttcgtcac tgaaagaaaa aaagtgtgct
     7561 gaaacagaaa tttcagttgt gagacctgac ggttcaaaag caacgctttc agtcattccg
     7621 aaaacaggaa catattctga tgtctttaat gaagagatag aaagatgtga gataggtatt
     7681 gcacggcttc cgagagacgc atccattgct ttgctcagtg aatttaaagg gttgcaaaac
     7741 ggtgacatta taatttcaat agacggagct cctgtttcaa ggttctacga agttgaaaaa
     7801 cggcttaaag gggcttcatt taaaactatc aaacttaaac ggaatgggga agaatatgcg
     7861 gtttcgttcg aaaatgaagc ggtgccgtca ctagttcacg gcgggcttat tgtctcaaaa
     7921 gttgaggaag gatccttttc ttccggaata ggtctggaaa aaggagacat aataaactct
     7981 gtgaatggga gaacaatcac gatcccttat caattctact caataatgca gaagctcaag
     8041 gaaggagatg atgtaacgat cgggcttgtc agaaactacg agaagaaaga aataattttc
     8101 aaagcatcct ttgaagaaaa agaaaatgcg atgaccggac ttactgacag aaacatcaaa
     8161 tggggagcaa atttcgcttt caattatgat gttcctgaga tctacgctaa aagagctgaa
     8221 ccttttaaat tcactttcaa atatgcttca gagcagactt tcggcatgat ctcaatgaca
     8281 ctcaaggggt tctggtatct tgtaacagga aaaatgtcgg cgaaaagtct cggcggccct
     8341 attatgatct ttgacatttc aaaaaaagcg gctgaagccg ggctcaaaca ctttcttttc
     8401 attcttgccg tcatcagcat aaatcttgga attattaatc tttttcctgt tcccatcctt
     8461 gacggtggtt acattgtgat gtacactatc gaagggattg tgagaaagca gatccctgtt
     8521 tcaataaagg agaaaatgct gaccgcagga cttgtaatgc ttcttctgtt aatggctttt
     8581 gccattttta acgatttttc aaggtatatc tcaattttta cgagggtgta attgaaaaac
     8641 ctgctcgttt ttgacggatc cacaaaaaac atacttttcg gaggaacgct ggaaaacagc
     8701 tgcatttttc attgcataaa ttttgaaaga tccaaaatga gctgccgact gcctgatttc
     8761 tttcttaatt tgaaacctga cattcgtttt gaaactttgc ctgaagtcat tatcggtaac
     8821 ggtcccggac ctttcactgg actgaaaacc gcaaattcgt tctttctggg ttttatctat
     8881 tcactgggtt tgaaaaaggt gcgccttgtc agttctttca agatcatttc cgcactgagc
     8941 ccggcagaag ggaacctaaa cagattagtg gtgatccctt ttaataaagg tgaatatttt
     9001 cttggacttc ttgataaaga gaacaatctg ctcgtaaaag atgtttttat aaaagaaagc
     9061 tctgaaaaca ttaaggatat tttcaaagac tatctgaact caaaaattga actcatcctc
     9121 ggttcagaat gcggcgcaac tcttattgaa gcactgcgat ctcattttga cattaactcg
     9181 acacatcagg aagaatatca tttcgaccat cagcggcagc tatctctgct tggagaagaa
     9241 ctgcttgata ttactaaaga tcctttcata cttaaccatg taattaatcc ggcggacatt
     9301 gataaaaact gtaacattta tgtttcaaac aattttgagg aggggtgaat gaaagcagaa
     9361 aatctttcca ctgaagttat tttagaaaag atcagcaaac tgaaagaaga acattccagg
     9421 tatgatgaac tttcaaatga gcttgcggca aaaaagacct ttacacccca cgatgaagtg
     9481 gaacttaata ccttgagaaa aaagaaactt atgaaaaaag acatgatcgc ctattatgaa
     9541 gggctgttga aaaatacaaa ataatccaaa agagtttcta tacaattttc ccgttgattt
     9601 ttaaatgttt acctatatac tttgttacag tgctggttaa aaatgagcgg tggttaaaat
     9661 gaaaaagtta gtttcagcgt ttttgtttgt atcttttttt gtaatttttt ctgcatgcgg
     9721 atcagatgat gaagaagaaa aaaaacagtg tatcgccgat tcagactgtc cgttccctta
     9781 taaatgcgat cttgagatat tccgctgtaa gcatcctgaa acaggagaac tgcttgatga
     9841 tggaaatgag tcaggggatt ccggcaactc cggaaattca ggcgactcgg gaaacaccgg
     9901 caatacaggg aattccggta attcatccaa cgatccgaat aaggacgatc cctgctttga
     9961 ccgtaatgct gatgccgatg gtgacggtgt gccgaataat gttgagtgcc ctttctttcc
    10021 gtgttctgac agtgacggag acggaaagcc gaactgtctt gaccttgact cagatgatga
    10081 cggaataagc gatgcagatg aaaagacaaa cggcaccaat ccgtacaatt ccgatactga
    10141 cagcgacgga ttgactgact atcaggagtc acagtatgga acaaatccta aaatgaaaga
    10201 cagcgactct gacggttttg atgatcttgc cgagatcgca tacggaaccg acccgaacaa
    10261 caatgccgac aaaatacctg aagatgtgtt ttatgtggtt cttccgtaca agccgggctt
    10321 tgaaacaaaa aaacagcttg atttcagcac agatattcag caggcggata tagcaattct
    10381 tgtcgatctt tccggctcca tgagcggaga gcacagcaat cttaagcagg gtattaacaa
    10441 tgtcataatt acaggtgtca aggcacagat acctaatacc ggattcggtc ttgtgaagtt
    10501 cggaacactt caggaccag
//
LOCUS       DAOOYB010000093        10472 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_6848_length_10472_cov_11.520015, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000093 DAOOYB010000000
VERSION     DAOOYB010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10472
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6848_length_10472_cov_11.520015"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="PL056_06975"
     CDS             <1..486
                     /locus_tag="PL056_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46794.1"
                     /translation="AEEKRLAEVSTPEVKRSVKTDDGEIRHLKVTTPQKEKTLSELLP
                     DLPVQKLEKKGALKNIYFRKFAEFSRVTMEVTGETDYQFREIKGGYVIDVHNFEKIPK
                     YLLNIIDTRGFEAEVEYIYPKKVGNILKIYIKTDQGMAVRKSEEENLINFDFFMPTIK
                     E"
     gene            483..3767
                     /gene="mfd"
                     /locus_tag="PL056_06980"
     CDS             483..3767
                     /gene="mfd"
                     /locus_tag="PL056_06980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HPM46795.1"
                     /translation="MTRFIETPADGFPVLVRELFKRHGKLLIEAPSFKDGSMLAGALS
                     AFDFEVVFFAPQFLLLERSGTDPDAQSTFSKIVSKTADVVVTTPLSSRMVIPDIENGR
                     LDLKTGLNCRISDIINSLVMSGYRKVAVVKEPGDFALRGDILDVGVFIPGKGVRLEFF
                     DDEIERMSLFWLSTQRNWKKIESINIPKLLFSNNLRTDWKSVLENKAANLSMKEILET
                     EDLISTGVFSKWDMYPIIAGDSTVDSVFEGTRVRWEKMRGEATFENDLAVLDKERKKR
                     VEEGHFIPFEISHCFKEISSFDIEVSTLFSIGSEIEKFQITHRKVAQRVKEEPAFILN
                     KIIKDEETAVLYAKPNERDPMIELCENSGLPVIPVERIPLETEAGTIYLTESREWFER
                     DSILFVPSTGCSFISSEIFGIPGSKKIKKEIVIPEYNENAVFELQALKTGEYIVHYNF
                     GIGIYEGTERMNGTDCLVLRYDRDDRLFIPVYNMHFVYKYRWEEGAFPRVSSLRTSTW
                     EITKSKVTKEVEEVAAKILELYAQRSVQTVEPLKVDTEMYNRFSSLFPYRETRDQRKS
                     ILELENDLSTYEITDRLLCGDVGFGKTEVAMRGCMIAVANGRQAAVLAPTTVLAFQHL
                     RTFIERFSKLPVKIEMLSRFYSDAKQREVLRDLKDGKVDIVVATHRLLSKDVEFKDLG
                     FLVVDEEHRFGVAHKERIKEMKKDIATLSMTATPIPRTLQMSLLGIRKVSFIKTAPGD
                     RKNIRTYVLEYSEEIIKEAILNEMSRNGQIYFVHNRIASLNDIKMKLENLIPGLKICI
                     AHGQMDEKQLENVMVDFVDRKYDLLLSTSLIESGIDIPMVNTIIINRADMFGMAQLYQ
                     LRGRVGRWNREANAYLFVPNLSTISPDSYARLAVIKRFDQLGSGYDVAMEDLNIRGGG
                     SILGFAQSGKLKGVGYDMYLEMLRKRIEELKTGVPHSETEFDISTDLFANIPETYIPD
                     TEMRVGFYRKIADIRSVQELNWVKETMTEMFGSIPPETENLIFLTNARLKAIQAGGSS
                     MTVNANSFSLTLAPAFIPENIEKLFQFIEKRKGTFSDSHTIKFSIRNMTEIDEIIEEL
                     SKISLG"
     gene            3880..4632
                     /locus_tag="PL056_06985"
     CDS             3880..4632
                     /locus_tag="PL056_06985"
                     /inference="COORDINATES: protein motif:HMM:NF036995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 N-terminal domain-containing protein"
                     /protein_id="HPM46796.1"
                     /translation="MVGKIQNLSEISDLILLLTETQNFFQKQVQKQVNTALTLRNWLF
                     GFYINEYEMNGLDRAEYGKGVMKEIAQRSVHIKGLSERNLYLFRTFYLSYPMILQSLT
                     AKSFLTDFRQIPIFHAVTGKLQLSYNVEQNQIVEDICGKLETNPEILINRLSFTHIIE
                     LLKADTLLKRFFYETETIKNNWSVRELQRAMNSLLYERTGMSKNENLVKYATAGLPQQ
                     VFVSKYLVNLPSESELKKIIQQEQDKISNREK"
     gene            4783..4998
                     /locus_tag="PL056_06990"
     CDS             4783..4998
                     /locus_tag="PL056_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46797.1"
                     /translation="MKNITIGLDARTFFLIKQANPKNLSKFIREAIAEKAQAELNRQI
                     DDYCANLKSNMEDFDGFNSESEENWPN"
     gene            4958..5320
                     /locus_tag="PL056_06995"
     CDS             4958..5320
                     /locus_tag="PL056_06995"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HPM46798.1"
                     /translation="MDSTANQKKIGQIKRGSIVLVQFNPSVGSETKNIHFAVVVSIDQ
                     LNSRSPLISVVPMTTKIERFFPAFEVMINETGKPAKADCAQVRTIAKERVVEIKGIVS
                     DETLVEIGNKIKTALGLD"
     gene            5385..6641
                     /locus_tag="PL056_07000"
     CDS             5385..6641
                     /locus_tag="PL056_07000"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM46799.1"
                     /translation="MFDSISKYQIWGKEPLSAGFPRTSATDTISKWIGNKLIKVISGQ
                     RRCGKSFLIRQIMNMLVDKKISKKESILYINMEYGVFNFIKNHEDLFNFALKFRKNLS
                     ENKKCFLFIDEVQKIDGWEKCVNSLAQDFTEDWEIFITGSNASLLSGELATLISGRYI
                     SYTLFPFSYSEFCGITESGHNAESFSRYMQTGGMPECFKLTDDETIRHYISNLRDSII
                     LRDIVDRYQVRDIRLLDQLIELIVDSVGSYFSVNKLANVITSNGGKTNSVTIGNYIEY
                     LKDVFFIHEVPRYDVAGKKILTGDRKFYINDPSFRYFSTMIRENTPGKYLENAVLLHL
                     LREGYSVQAGSISGKEIDFVAKKNNSILYVQAAYILSNENVISREFGNLEMIKDNFPK
                     IVVTLDPVSFGNINGIEHFRAWEWVK"
     gene            complement(6654..7574)
                     /locus_tag="PL056_07005"
     CDS             complement(6654..7574)
                     /locus_tag="PL056_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPM46800.1"
                     /translation="MKRVLLATDKPFAAKAVSKIQEICDKAGYQLVKLESYTSPDQLK
                     EAVKDVHAIIVRSDLITREIMDAAPELKIAVRGGAGFDNIDCKAAAEKGIVVMNTPGV
                     NANAVAELAIGMAVYVARGLFTPKTGGELLGKTIGIHAYGNVGKNVARIAKGFGMEVY
                     AFDPFVKKEAIETDGVKVVDTVEDLYKKCQYVSLHIPANDKTKKSINKNLIALMPKGA
                     TLINTARKEVVCEEGLKFVFESRPDFKYVTDVAPDNAAELAEKFAGRIFCTPKKMGAQ
                     TNEANDNAAEFAANNIVNFFEKGDITYKVN"
     gene            complement(7687..7977)
                     /locus_tag="PL056_07010"
     CDS             complement(7687..7977)
                     /locus_tag="PL056_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009147409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HPM46801.1"
                     /translation="MNIYVGNLAYNLSEDELKAAFEAFGKVESARFVTDRDTGRSKGF
                     GFVEMPNKDEATKAINALNGTELQGRTLTANEARPREERAPRTGGGNYGKKY"
     gene            complement(8179..10086)
                     /locus_tag="PL056_07015"
     CDS             complement(8179..10086)
                     /locus_tag="PL056_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009622582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HPM46802.1"
                     /translation="MIYTTSPIAIGLFLFFVSFVLFLSFYFAKKTKTSASYFAAGGTI
                     HWAVNGIAFAGDYLSAASFLGICGMIAYYGFDGFLYSIGYLAGWVVALFVVAEPMKRL
                     GKYTFTDALDSQFNSKSIQLLATISTLVVSICYLIPQMVGAGALVTPLLGLPHWVGVV
                     MVGAIVIFIVATAGMTSTTYVQFIKGGLLIVFSTILSVYIFSNGINLKPHADYHEFKT
                     IPVVVDENGEVSISDSEYAFAGKTVVKGDTFVKLIKGSVASWWKLKESYLEETIFIEK
                     TSEGKLLFNGEPQEKGKFYSVGHASRIVVDGKEVEKSGHLGPGSFLSTIDNSEIVRFL
                     PVKFKHNGNSVSLWQQTVTKGSKIMRPGLLFKIDGNSTFLQKLNFISLMLALFLGTAA
                     LPHILIRYYTVPSQKDARKSTIVAIAAIGFFYILTLYMGLGAMVNGVLDVESSNMSAP
                     LLAKFFGVGLFSIISAIAFATVLGTVSGLIVAASGAVAHDLMDRFLAIKMTDTQKVRA
                     GKMSAVVVGALAIIMGIIFKGVNVSFLVGLAFAVAASANLPAILMLLFWKGTTAKGIA
                     ASIFVGMTASLSIIALSPTMYEVYGLDPATAIIPLSNPGIISIPLSFITLVVVSIITK
                     KDNSTKISAPV"
     gene            complement(10083..10385)
                     /locus_tag="PL056_07020"
     CDS             complement(10083..10385)
                     /locus_tag="PL056_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013506858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HPM46803.1"
                     /translation="MLHGPAAKLGKDNASAVKSKLGLKLFAVYALIYAGFVVINMVDP
                     NMMEMKIVAGLNLAVVYGFGLIILAIIMGIIYNGICTNCEDSMNKPEKNTEKGGEK"
BASE COUNT         3294 a         2036 c         2199 g         2943 t
ORIGIN      
        1 gctgaagaaa agaggttggc tgaagtatcc accccggaag taaaaagatc agtaaaaacc
       61 gatgacggag agataagaca tcttaaagta acaactccgc agaaagaaaa gaccttatca
      121 gaacttcttc cggatcttcc tgttcagaaa cttgaaaaaa aaggtgctct taaaaatatc
      181 tatttcagaa agtttgctga attcagtcgt gtcacgatgg aagtgacagg agaaacagat
      241 tatcagttca gagaaataaa gggtggttat gtaattgatg ttcacaactt tgaaaagatc
      301 ccgaaatacc tgcttaacat cattgacacg aggggctttg aggcagaagt cgagtatata
      361 tatcccaaaa aagttggtaa cattctgaaa atatacataa aaacagacca gggaatggca
      421 gttagaaaaa gtgaagaaga aaacctcata aacttcgatt ttttcatgcc gaccatcaaa
      481 gaatgacaag attcatcgag acaccggcag acggatttcc tgttctggtc agagaacttt
      541 ttaaaagaca cggaaaactt cttatcgaag caccgtcatt taaagacggg tcaatgcttg
      601 ccggagcact ttcggcattt gattttgaag ttgttttttt tgctccccag tttcttcttc
      661 ttgaaaggag cggaactgac cccgatgccc agagcacatt ttcaaagatc gtatcaaaaa
      721 cagccgatgt tgttgttacc acacctcttt cttcgagaat ggtcattcct gacatcgaaa
      781 atggacggct cgatcttaaa accggcttga actgcaggat atcagacatt ataaattctc
      841 ttgtcatgtc cggatacaga aaagtggctg ttgtcaagga accgggggac tttgctttaa
      901 gaggcgacat tctcgatgtc ggtgttttta ttccggggaa gggagtgcgg cttgaattct
      961 ttgatgatga aatcgaacgg atgtcccttt tctggctttc gactcagaga aactggaaaa
     1021 aaattgaatc aataaacatt cccaaacttc ttttttcaaa taatctaaga accgactgga
     1081 aaagtgttct tgaaaataaa gccgccaatt tatcgatgaa agagatcctt gaaactgaag
     1141 atctgataag cacaggagtt ttttcaaaat gggacatgta tcctattatt gccggggaca
     1201 gcacagttga ttcagttttt gaaggaacaa gggtccggtg ggagaaaatg aggggtgaag
     1261 caacttttga aaatgatctt gctgtgcttg ataaagaaag aaaaaaaaga gttgaagagg
     1321 gtcatttcat cccttttgaa attagtcact gtttcaaaga gatctcctca ttcgatattg
     1381 aagtgagcac cctcttttca atcggttcgg agatagaaaa attccagata acacacagaa
     1441 aagttgctca gcgagtaaaa gaggagccgg catttattct taacaaaata ataaaagatg
     1501 aagaaactgc ggtactttat gcaaaaccca atgaaagaga tccgatgatc gaactttgtg
     1561 aaaactccgg gcttccagta atccctgttg aaagaatacc tcttgaaaca gaagcaggaa
     1621 ctatttatct tacagagagc agagagtggt ttgaaagaga ttcaatcctt tttgttcctt
     1681 ctaccggctg ttcgttcata tcgtcagaga tcttcggaat tcccggttca aaaaaaataa
     1741 agaaagagat cgtcattccg gaatataacg aaaatgcagt ttttgagctt caggcgctga
     1801 aaaccggtga atacatcgtc cattataatt tcggaatagg aatttacgaa gggactgaaa
     1861 ggatgaacgg aaccgactgt ctggtgctgc gttacgatcg cgacgaccgt ctttttattc
     1921 ctgtttacaa catgcacttt gtctacaaat accgctggga ggaaggggct ttcccgcgag
     1981 tcagctcgct taggacttca acctgggaga tcacaaagag caaagtgacg aaagaagttg
     2041 aagaagttgc ggcaaaaatt ctcgaacttt acgctcaaag aagcgttcag accgttgaac
     2101 cgctcaaagt tgatacagaa atgtacaacc gtttctcttc gctgtttcca taccgtgaaa
     2161 caagggatca gagaaaatca atacttgaac ttgagaacga tctttcaaca tatgagataa
     2221 ctgaccgtct tctctgcgga gatgtcggct tcggcaagac cgaagttgcg atgagagggt
     2281 gcatgatcgc agttgcaaac ggcagacagg cggcggttct tgctccgaca acagtccttg
     2341 cttttcagca tcttagaact tttattgaga gattttcaaa acttcctgtg aaaattgaaa
     2401 tgctttcaag gttttacagc gatgcaaaac agagagaagt ccttcgtgat ctcaaagatg
     2461 gtaaagtgga tattgttgta gccactcacc gtctcctttc aaaggatgtc gagttcaaag
     2521 atcttggttt tctcgtggtt gatgaggaac acaggttcgg agttgcccac aaggaacgga
     2581 taaaggagat gaaaaaagat attgcgacat tgtcgatgac ggcaacaccc attccaagaa
     2641 ctcttcagat gtcgcttctt ggaataagaa aagtctcttt cattaaaacc gctcccggcg
     2701 acagaaagaa tatcagaacc tatgtgcttg aatatagtga agaaataatc aaagaagcga
     2761 ttctaaacga gatgtcaaga aacgggcaga tatattttgt ccacaatcgg atcgcatctc
     2821 tgaatgacat aaaaatgaag ctcgaaaatc tcattcctgg gctgaaaata tgtattgccc
     2881 acggtcagat ggatgaaaaa caacttgaaa atgtgatggt cgattttgtt gacaggaaat
     2941 atgatctgct cctttcaact tcgcttattg agtcgggaat tgatattccg atggtgaaca
     3001 ctatcataat caacagagcg gatatgttcg gaatggctca gctttatcag ttgcgcggca
     3061 gggtcggcag atggaacaga gaggcaaatg catatctttt tgtccctaat ctcagcacga
     3121 taagtcccga ttcatatgca cggcttgcag ttataaagag attcgatcag cttggatcgg
     3181 gttatgatgt cgcgatggaa gatctcaaca ttcgcggtgg aggcagtatt ctcggttttg
     3241 cccagtcagg aaaactcaaa ggtgtcggat atgatatgta tctggaaatg ctgagaaaaa
     3301 ggattgaaga gcttaaaacc ggcgtgccgc acagcgaaac tgaatttgac atttcgacag
     3361 acctttttgc caacattccc gaaacttata ttccagacac agaaatgaga gtcggttttt
     3421 atagaaagat cgcagacatc cgttcagttc aggagctcaa ctgggtcaaa gaaaccatga
     3481 cagaaatgtt cggcagtatc ccgcctgaaa cagaaaacct catcttttta acaaatgcac
     3541 ggcttaaagc aatacaagcc gggggctcat caatgactgt caatgcaaac agcttttctc
     3601 ttacacttgc acccgcattc atccccgaaa acatcgaaaa actctttcaa ttcatcgaaa
     3661 agagaaaagg aacattctca gattcccaca caataaaatt ctcaatccgc aacatgaccg
     3721 aaatagatga aatcattgaa gaattaagca agatcagttt gggttgaaaa cttacgcact
     3781 caatcaattt ctttaattta caatttctta tccaatttct tgcatattta atttgattgt
     3841 tgcattatct attccagact aaacttttgg agtcggaaaa tggttggcaa gattcaaaat
     3901 ctttcagaga tatctgattt aattcttttg ttgacagaaa cacagaactt ttttcaaaaa
     3961 caggtgcaaa agcaggtaaa tactgctctc acgctcagaa actggctgtt tggattttac
     4021 attaatgaat atgaaatgaa cggattggac agagctgaat acggaaaagg tgtgatgaaa
     4081 gaaattgcac aaagaagtgt acacattaaa ggattatccg aaagaaacct atatcttttc
     4141 agaacattct atctttcata tccaatgatt ttgcagtcac tgactgcaaa atctttttta
     4201 actgatttca ggcagatacc aattttccat gcggtgactg ggaaattgca gttgtcatac
     4261 aatgtcgaac aaaatcaaat agttgaagat atttgtggca agcttgaaac aaatccggaa
     4321 atattaataa acagattgag tttcactcac ataattgagc tgcttaaagc ggacaccttg
     4381 ttaaaaagat tcttttatga aacagaaaca ataaaaaata actggtcggt gcgagaattg
     4441 caaagagcta tgaacagttt gctttatgaa agaacgggaa tgagtaaaaa tgaaaatctg
     4501 gtaaaatatg caacagccgg attgccgcaa caggtctttg tctcgaaata tcttgtgaat
     4561 ctcccatccg aaagtgaatt gaagaaaata atccagcagg aacaggataa gatctcaaat
     4621 agagaaaaat aaaatggttc ggtttttcca aaatggatgt tgaagttgcg aagatggtga
     4681 tgagagtatg atattctatt gcccgaatca tacatataaa ttactgttga cacattgctg
     4741 tgtatctgat agtattgtga aagatgtttt cggaggtgtg tcatgaaaaa cataacaatc
     4801 ggtcttgatg caagaacttt ttttctgatc aagcaggcca atccaaaaaa cctgagcaaa
     4861 ttcattcgtg aagcaattgc agaaaaggcg caggcggaat taaaccgcca gattgacgat
     4921 tattgtgcca atttaaagag caatatggag gattttgatg gattcaacag cgaatcagaa
     4981 gaaaattggc caaattaaac gagggtcaat agttttagtt cagtttaatc cttcagtcgg
     5041 gtcggaaacc aaaaacatcc atttcgcggt cgtggtttca attgatcagc tgaacagccg
     5101 ctctccacta atttcggttg tgccgatgac aactaaaata gagagatttt ttcccgcatt
     5161 tgaagttatg ataaatgaaa ccggaaaacc tgcaaaagct gattgtgctc aagtcagaac
     5221 aatcgcaaaa gaacgagtcg ttgaaatcaa aggcatcgtc tcagatgaaa ccctggtcga
     5281 gatcggaaac aaaatcaaaa ctgctttggg actcgattaa agcatacttt acaatagtgt
     5341 ttttttgtgg taaagtttaa agtatgtttt aagcggaggt tgtcatgttt gattcaataa
     5401 gcaaatatca gatctgggga aaagaaccgt taagtgccgg atttccaaga acttctgcga
     5461 ctgacaccat ctcaaaatgg atagggaaca aactgataaa ggttatcagc ggacagagaa
     5521 gatgcggaaa aagttttctg atccgccaga taatgaatat gctggttgat aaaaagattt
     5581 ctaaaaaaga atcaatactt tacataaata tggaatacgg tgtcttcaac tttattaaaa
     5641 accacgaaga tcttttcaat tttgcactta aattcagaaa aaacctttca gaaaacaaaa
     5701 agtgttttct tttcattgat gaagttcaga aaattgatgg ctgggaaaaa tgtgtcaact
     5761 ctcttgctca ggattttaca gaggattggg aaattttcat aaccggatca aatgcctctc
     5821 ttctttcggg tgaactcgca actttaattt cgggcagata catttcatac actctttttc
     5881 ccttcagcta cagtgagttt tgcggcatta cagaaagtgg acataatgcg gaatcctttt
     5941 cgcgatatat gcagacgggg ggaatgcctg agtgttttaa acttactgat gatgagacaa
     6001 taagacacta tatttcaaat ctgagggatt ccataatttt aagggatatt gttgaccggt
     6061 atcaggtgag ggatattcgt ctgcttgatc aacttattga attaattgtt gattctgtcg
     6121 gaagttattt ttctgtaaat aagctcgcaa atgttatcac cagtaatggc gggaaaacca
     6181 actctgtcac tatcgggaac tatattgaat atttgaaaga tgttttcttc atacacgaag
     6241 taccaagata tgatgtggca gggaagaaaa tacttaccgg ggacagaaaa ttttatataa
     6301 acgacccgtc tttcagatat ttcagcacga tgatccgtga gaacacaccg gggaaatatc
     6361 ttgaaaatgc agttctgctt cacctcttaa gggaaggata ctccgttcag gccggttcaa
     6421 tttcagggaa agaaatagat tttgttgcaa agaaaaataa ctccattctc tatgtccagg
     6481 cggcatatat tttaagtaat gaaaatgtta tctcccgtga attcggcaat cttgaaatga
     6541 tcaaagataa cttccctaaa atagttgtca cactcgaccc tgtctctttc ggtaacatta
     6601 acggaattga acacttccgt gcatgggaat gggtgaagta accggtgatt taatcagttc
     6661 actttgtagg tgatgtcccc tttttcaaag aaatttacga tgttgtttgc ggcaaattct
     6721 gcggcgttgt cgtttgcttc attagtctga gctcccattt tcttcggtgt gcagaatatt
     6781 cttcctgcaa acttttcagc gagttctgcg gcattgtcgg gagcgacatc ggtcacatat
     6841 ttgaaatcgg gtcttgattc aaaaacgaat ttgagccctt cttcgcatac tacttccttt
     6901 cttgctgtgt ttataagtgt tgcaccttta ggcatgaggg cgataaggtt tttattgatt
     6961 gatttcttag ttttatcgtt tgccggaatg tgcaaagata catactgaca ttttttgtag
     7021 agatcttcaa ctgtgtcaac aacttttacg ccgtctgttt cgatagcttc ctttttaacg
     7081 aaaggatcga acgcataaac ttccatgccg aatcctttgg ctatacgggc aacattttta
     7141 cctacattgc cgtaagcatg aattccgata gtttttccaa gaagctctcc gccggttttc
     7201 ggggtgaaaa gtcctcttgc aacataaact gccataccga tcgcaagctc tgcgactgca
     7261 tttgcattta ctccgggggt gttcatgacc actatccctt tttcagcagc ggctttgcag
     7321 tcaatgttat caaagccggc accgcctctt acagcgatct taagttctgg agcggcatcc
     7381 atgatctctc tggttatcag atcacttctg actattattg catgaacatc ctttaccgct
     7441 tctttgagct ggtcaggtga agtataggac tcaagtttga caagctgata ccccgccttg
     7501 tcacaaattt cctgaatttt tgatacagct tttgcggcaa aaggtttgtc ggtagcaaga
     7561 agaactcttt tcatatcatc ctcctaaaat taattaaaaa acagaaccct aaagaagcta
     7621 atactattaa aaatggtttg tcaaatatta ttgtgagtga ggacttttag aagttaaaag
     7681 gggggatcag tatttcttac cgtaattgcc gccaccggtt cttggcgctc tttcttcgcg
     7741 tggacgagct tcattggcag taagtgttct gccctgaagt tcagtgccgt taagagcgtt
     7801 aatggccttg gttgcttcat ctttgttcgg catttcgaca aaaccgaagc cctttgagcg
     7861 gccggtgtca cgatcagtta caaatcttgc tgattcaact tttccaaatg cttcaaaagc
     7921 tgccttgagt tcgtcttcgc ttaaattgta agcgaggttt ccaacataaa tgttcattct
     7981 ttctctctca aaaacttacc atggttaaat aaaattactg gacgactgtc catggtgtga
     8041 ccagagatcc ataactaaat ccgatgttgc cttccaaact atttctgatt ggcagcacgc
     8101 aaaagttata caacaattac agtgaatgtc aatctattaa aaatattaaa gattttttcg
     8161 cagagtcttt agctttatct ataccggagc ggaaattttt gtgctgttgt ctttttttgt
     8221 gattatagaa acgacaacca gggtaataaa actcagcggt attgatataa ttccgggatt
     8281 gctcagcgga attattgctg ttgctggatc aagtccgtaa acttcataca ttgtcggaga
     8341 aagggcgatg atcgacaagg aagcagtcat gcctacaaat attgaagccg caataccttt
     8401 ggcagttgtt cctttccaga aaagaagcat gagaatcgcg ggaagatttg ccgatgcggc
     8461 tactgcaaaa gcaagcccca caagaaaaga gacattcacc cctttaaaaa ttattcccat
     8521 tatgattgca agagctccga caacgacagc tgacattttt cctgccctga ctttctgtgt
     8581 gtcggtcatt tttattgcaa ggaatctatc cataagatca tgtgcgacag ctcctgatgc
     8641 ggcaacgatc agaccgctta cagttccaag aacggttgca aatgcaatcg ctgagattat
     8701 tgaaaaaagt cctactccga aaaattttgc aagaagcggt gcagacatgt tagaactttc
     8761 aacatcaagc actccgttta ccattgctcc aagacccatg taaagagtga gaatatagaa
     8821 aaatccgatt gcggcaatag ctacgatcgt tgatttcctg gcatctttct gacttggaac
     8881 agtgtagtat ctgatcagaa tatgcggaag tgccgcagtt ccaagaaaca gtgcaagcat
     8941 aagcgaaata aagttaagtt tctgaaggaa agttgaattg ccgtcgatct taaaaagaag
     9001 accgggtctc atgatctttg agccctttgt tacagtctgc tgccacaaag acacggagtt
     9061 tccgttgtgc ttaaacttta ccggaagaaa acggacgatt tcactgttgt cgattgttga
     9121 taaaaatgag cccgggccga gatgtcccga tttttcaact tcttttccgt caacaacgat
     9181 cctgcttgca tgaccgacgg aatagaactt ccctttttcc tgaggttccc cgttgaaaag
     9241 aagtttgccc tctgaagtct tttcgataaa gatcgtttct tcaagatagc tctctttaag
     9301 cttccaccag cttgcaacac tgcctttgat cagtttgaca aaagtgtccc ctttgacaac
     9361 agttttgccg gcaaaagcat attcgctgtc agagatcgaa acttcaccgt tttcgtcaac
     9421 aactacggga atcgttttaa attcgtgata atctgcatgc ggtttaaggt taatgccgtt
     9481 gctgaaaata taaacagaaa gaatagttga gaaaactatc agaagaccgc ctttaatgaa
     9541 ctggacatag gttgttgatg tcattccggc agttgcaaca ataaaaataa cgatggcacc
     9601 taccatcaca actccaaccc agtgaggcag tccgagaagc ggtgtgacaa gtgcaccggc
     9661 tcccaccatc tgcggaatga gatagcagat cgaaacaaca agcgtgctta ttgtggcaag
     9721 aagctgaatg ctttttgaat tgaactgtga gtcaagtgca tcggtaaaag tatatttgcc
     9781 aagccgtttc atcggttctg caacaacaaa aagggcaacg acccagccgg caagatatcc
     9841 gatggaatag aggaatccgt caaatccgta gtaagctatc attccacaga tgcctaaaaa
     9901 tgaagctgcg gaaagataat caccggcaaa agcgatcccg tttacagccc agtgaatggt
     9961 tccccctgcg gcgaaatatg aagcagatgt tttggttttc ttggcaaaat aaaaactcag
    10021 gaaaagaaca aacgaaacaa aaaataaaaa gagccctatg gcgataggag aagttgtata
    10081 aatcattttt ctcctccctt ttcagtgttt ttttcaggtt tgttcatact gtcttcacaa
    10141 tttgtacaga tcccgttata aattatcccc atgattatgg cgagaattat cagtccgaat
    10201 ccgtaaacta cagcgagatt aagaccggca acaatcttca tctccatcat attcggatcg
    10261 accatgttga taacaacaaa tccggcatag atcaaagcat agacagcaaa cagcttgaga
    10321 ccgagttttg atttcacggc agaggcattg tctttgccga gttttgcagc aggtccatgt
    10381 aacatttttc cctccgggac aaacagtttt tgcaattttt taaacagcca gcggattgaa
    10441 aaatatgccg aaaaatgaat ttgtcaaaga aa
//
LOCUS       DAOOYB010000094        10397 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_6918_length_10397_cov_12.072617, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000094 DAOOYB010000000
VERSION     DAOOYB010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10397
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6918_length_10397_cov_12.072617"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1696)
                     /locus_tag="PL056_07025"
     CDS             complement(<1..1696)
                     /locus_tag="PL056_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46804.1"
                     /translation="MKRFCLFILLGMVLFSCSDKKSNHFNDYDIVDTGQPDKEDLPGD
                     NNSLPDENNDNDVDTEIQERECETVCGKGTEQFIDGEWKNCTAPKDKDKRDCEKMNTY
                     GTCLGFEECDGTNGWGECDAKEPEPEYYDGIDNNCNDEIDENIINPVTDGTVDEGLEK
                     IDENDPENPTGEDGLTLSRKSKVLPYLWAANHEYQSVSKFNTQTLKEEGRYWVGINPS
                     RTAVDLDGNMWVGGRDDGRVTKVLWDTTQCPDSNGDGIYTSYIDGTTGEVVQVNSGTD
                     PLTDPFVDECVVYSAVTNPSFPSIRGMAVGSDGKIWIGYSYGGVQSIDPETFALGTYH
                     SAAAVPVWRPDEFGVFQVVTGEMVNADGVYGLIVDSNGILYISSLTNRRYLVAFDTKT
                     ETWIGAFEKDGACSYGIAVDGKNRIWLGGYPECMGIEMFDFEQKKAWSFLVPDSATLT
                     PGSTSGVLVHEKAAGGGKAAHDTTGITVDPATGNIWTSFYLQGYTGKLVLDETDFANS
                     SWTFIATARDALNNLLPGVGVDLRGVGFDPYGYAWTLGLGSGKVWKIDPATNARAAD"
     gene            1817..3805
                     /locus_tag="PL056_07030"
     CDS             1817..3805
                     /locus_tag="PL056_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46805.1"
                     /translation="MKNLIVFVTIVVALSGCEEKKKIDMSDACKSGHYSQCKEGDVYL
                     FNICGEEIGKIEECSENEKCEEAECVPCEETSACESKGCYEGNVYCFSNCGKRKMLVK
                     ECEDGTTCSNGKCVECEPVELCVEKKCYDGNVFCYTDCGNRGELDEVCAQTEVCEGGA
                     CLKCDVTEPCETKKCVDSVVYCYDNCGKEGELFQECESYETCEDGECNCTPDCNGKNC
                     GTDGCGGTCGECTGTDECVSGTCGSKVDMEGPEYKGDVLLIINTGTNGSQSSFSGTLP
                     LPVLKDNIVSKSDGTSFVRKYFDPGVRIPGDLDLSQRPAKIIRSVPAPAMDPVAGDTQ
                     NFWVADFGSGGDREITATLQHISSKCEVWAEYPSITGTTKARQICEEFDSVIYPMVTT
                     NFHPESDINSDGRIAFLIADLGGFAAGYFTPADFYSKEEYYGSNERDMVYIEQDMDIS
                     QIRNTIAHEFQHLCYANRNFLIEQDNNYSDLDVRWIDEGLATASEHLYGGSQSDWVYI
                     FNTSPYNDPVAQGNGFFKWDYSDTNRVYSDYALTYAFFQYLRLQSPDQDGIYNEIILN
                     TKNDWDGVQDVIRSRIDGDINFSDFMTGFRIAMLLNDAAGPYGFMGDTGLKFTPKFYT
                     GSGRNVEGGGALYFKISGSFTEPADKGAGIIYVGITTK"
     gene            3874..4347
                     /locus_tag="PL056_07035"
     CDS             3874..4347
                     /locus_tag="PL056_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46806.1"
                     /translation="MNNQIFKIISAVFVSGLLFSCGGPESYKTPIVNLGDIIQGECNP
                     VGEGDLLEESFSAEVVNGDVVVEHLNILVPESTMMGIIDTDSNSSLDGKFNYYYLEAS
                     EQFISVREIFRRSSSEKNCYYDLRIKITNVSGGIYDLMLFNETGKLKFSKEIRVR"
     gene            4344..5567
                     /locus_tag="PL056_07040"
     CDS             4344..5567
                     /locus_tag="PL056_07040"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF005154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HPM46807.1"
                     /translation="MNLSPEKVIVLKFGGSSLKDDETLQRAVSIISSRVLDGYKVVAV
                     VSARGKTTEALLKDASVFAPAGDPREVDMLLTTGERASAALVAMALKSKGIPAISFTG
                     SQAGIITDDNHTNARIIEIRPDRVINALNEGMTVIVGGFQGVSFKKEVTTLGRGGSDT
                     TAVALAAALNAEICEIFSDVDGVYSADPNLVADAKKIDQLSYQEMQELSEAGAKVLHS
                     RAVEFAKEKGIEIHCKSTFEPDNPGTVIRGFETREKIRKAGVTYEKNVILVHAYEPDA
                     FGLPTINNVLKFCRERNVSVKQISFHKGYKESMVGSFILSKIENYSCDAFISELTTKF
                     EGAVEIFKELSAVSIVGEGITDRPDTLLEAIELLKNNEIQVSGFHTSSFRISMLINFA
                     LLEKAVKLLHEYCRA"
     gene            complement(5564..6811)
                     /locus_tag="PL056_07045"
     CDS             complement(5564..6811)
                     /locus_tag="PL056_07045"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HPM46808.1"
                     /translation="MRILLITILFVALSGCDTFKLEHMGGVGESCFNNDTCTGDLVCV
                     YGVCEELDESDESDQSEVSDQSEVSDQSEFPDESDQSEFPDESDDSDQSDEDLIEDLC
                     NFGEICWKVVPTQQKRCFNETGTTNCTEVTSTFYGQDGLYADSSTRGFENIKFGDKYF
                     VFDNRTKLLWYKTASENPVNFSDAQAFCEILNSTSQGERTNWRIPYLHELVSIVNFDE
                     TIDPLVDGFYFPFLKKEKYWTLTKLGGNNKYYVDFSGSSQFYVDDGSSKMNSAICVSS
                     DHIYDTERKFKRFAQTVINENTIVEDKLTGLQWQKVTDYSARIWKDALAYCESLSFAG
                     KTDWRLPNVNELHSLATYEIDYELQTVFPDLGDDFFWSSTTNANVIQNAWVVEFKYGI
                     LKPELPKDKNENQIYTMCVRNKD"
     gene            complement(6813..7652)
                     /locus_tag="PL056_07050"
     CDS             complement(6813..7652)
                     /locus_tag="PL056_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46809.1"
                     /translation="MVLKRCLMFAVSFLFALELFPNDVENDSIVVWRIEPKTGVTDKE
                     ADAISGIVTAEVGRVSGRKTVGENEMKALIVGEEMKMSCGAEDTACVAEIGAALGAPE
                     SITGTISKMGDYWILTLQRLNVRNVEVISRYENRVKGDVNMIVEMIAPAVRELFGIKD
                     AAKDEKIVKKEKKPKKKMSVLMKSGIGVMVAGGVVLAFGGIATWRTIVEKENYQEGKT
                     DGDMSKYNAWKYTSITSYTVGGVAFATGVALQIADVFIDAPVKTAVIPVPGGAHVYLS
                     WRW"
     gene            7904..8995
                     /gene="pyrB"
                     /locus_tag="PL056_07055"
     CDS             7904..8995
                     /gene="pyrB"
                     /locus_tag="PL056_07055"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HPM46810.1"
                     /translation="MNYLDQQFENFKNLPIEKKIELFEMDGKLFDMIISQQLSREVLE
                     TLYNLTNHLRRMGKLKEGLDFLATLLSHKRAMLYFEQPSSRTFLSFLNACHILGLKTS
                     EIRDTSVSSEVKGESYFDSIRTFSSYTDLVIMRSKNKDAAEKAAWLLNTYSNRPVPII
                     NGGSGADQHPTQALLDIYTLMRSFANQGGLDGKTVMFCGDLRRGRTARSLAYLLERFN
                     DVKLIFCAPKEFQILDDILESLDRKGVKYEIITESVRSGLDRADAIYMTRIQDEHDLV
                     AGESSTIDISKFKLSGEDLKYMKPGAAILHPFPRRDEIATEIDSDPRAKYWRQGRNGM
                     WTRVALIAYVFNLEKQIMEFDVSRVDHLR"
     gene            complement(9069..10001)
                     /locus_tag="PL056_07060"
     CDS             complement(9069..10001)
                     /locus_tag="PL056_07060"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPM46811.1"
                     /translation="MLTNSRTSAMGYAMLYALMNLGGFLPGLISPPVRKSFGISGVFW
                     VYSILTVLGLAVVAIFITKKALKDAIDQARADEPDKTLLEKEETDEEKEKMSATEKMK
                     FYLKNFPITDARFMFFIFVLIPVQTLFAHNWLTLPQYTSRAFDGFVGDNFEFFVNLNP
                     ILIFILTPMVTAMTAKKDTYKMMIIGTFIMAAPTFILALGPSMGTLMTYLVIMTIGEA
                     IWQPRFLQWVAQIAPKGMTGIYMGIGQFPWFLTKVVTAIYSGWFLMKYCPADTAPSQM
                     NTETMWFIYGLIAMISPFALLLASKWMKKGFKTT"
     gene            complement(9901..10221)
                     /locus_tag="PL056_07065"
     CDS             complement(9901..10221)
                     /locus_tag="PL056_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46812.1"
                     /translation="METNTEQKKDSMVKELIQPFLDLGKTSRPLLGLNISYVIEGLVY
                     FGIVGLLALFFNEYIKLNDIDAGGIFGCKNADKQQNFGNGICNALRFDEPWRISAGAY
                     FSAG"
BASE COUNT         3030 a         2166 c         2195 g         3006 t
ORIGIN      
        1 gatctgctgc tcttgcgttt gttgcaggat cgatcttcca tacttttccg cttccaagtc
       61 cgagtgtcca tgcatacccg taagggtcaa aaccgacacc gcgaagatca actccgacac
      121 ccggaagaag attgtttaaa gcatctcttg cggttgctat aaatgtccat gaactgtttg
      181 caaaatcagt ttcatcaaga accagttttc cagtatatcc ctgcagatag aaagaagtcc
      241 agatgttgcc tgttgctgga tctaccgtta ttcccgtcgt gtcatgagcc gcctttccgc
      301 caccagccgc tttttcatga accagaactc ctgatgtcga tcccggagtc agagttgctg
      361 aatcgggaac aaggaaagac cacgcttttt tctgctcaaa gtcaaacatc tctattccca
      421 tgcattcagg ataaccgcca agccatattc tgttctttcc atcaacagca attccataag
      481 aacatgcacc atctttttca aatgcaccta tccatgtttc ggttttcgta tcaaatgcga
      541 caagatatct tctgtttgtg agagagctta tgtacagtat tccgtttgag tcaacgataa
      601 gtccgtaaac tccgtcagca ttgaccattt cgcctgtgac tacttggaaa acaccaaatt
      661 catcaggtct ccatacagga accgcagctg cagaatgata tgtgcctaaa gcaaaagttt
      721 ccgggtcgat tgactgaact ccaccatagc tgtatcctat ccatattttt ccatcactgc
      781 cgacagccat tccgcggata ctcggaaatg aggggtttgt tacagcactg taaaccacac
      841 attcatcaac aaaaggatca gtcaaagggt ctgttccgct gttaacctga acaacttctc
      901 cagtcgttcc gtcaatgtaa gaagtgtata ttccgtcacc gttactgtca ggacactgag
      961 tcgtatccca gagaactttt gtaacccggc cgtcatctct tcctccgacc cacatattgc
     1021 cgtcaagatc gaccgctgtt ctgctcgggt ttattcccac ccagtagcgg ccttcctctt
     1081 ttaaagtctg agtgttgaat ttgctcactg attggtattc gtggtttgcc gcccataaat
     1141 aaggaaggac ttttgatttt ctggaaagtg tcagtccatc ttctcctgtg ggattttcag
     1201 ggtcattttc atcaattttt tcaagacctt cgtcaactgt tccgtcagtt accggattga
     1261 taatattttc atctatttca tcattgcagt tgttgtcaat accgtcataa tattccggct
     1321 cgggttcttt tgcatcgcat tcaccccaac cgttcgttcc gtcacattct tcaaatccca
     1381 agcaggttcc gtaagtgttc attttttcgc aatctctttt atctttgtct tttggtgctg
     1441 tgcagttttt ccattctccg tcaataaact gctcagttcc tttgccgcag acagtttcac
     1501 attccctttc ctgaatttct gtatcaacat cgttatcgtt gttttcgtcg ggaagagaat
     1561 tgttgtcccc cggaagatcc tctttgtccg gctgaccggt atcaacgata tcgtagtcat
     1621 taaaatgatt gctttttttg tccgaacatg aaaacaagac catgcccaga agaataaata
     1681 aacaaaacct tttcattttc ccctccctga taaaacttcc cgctcggtaa aaattatagt
     1741 agttatttta tttgttgcaa atttttcaac agacggtaac attttaaagc attgaatgaa
     1801 attcaggaga ttagagatga aaaatctgat tgttttcgtg accatagtgg ttgcattatc
     1861 tggctgtgaa gaaaaaaaga aaattgatat gtccgacgca tgcaaatcgg gtcattattc
     1921 acagtgcaaa gaaggcgatg tctatctttt caatatctgc ggtgaagaga tcggaaagat
     1981 cgaagagtgc agcgagaatg aaaaatgtga ggaggccgag tgtgttccct gcgaagaaac
     2041 atcagcatgt gaatcaaaag gatgttatga aggcaatgtt tactgtttca gtaactgcgg
     2101 aaagagaaaa atgcttgtaa aagagtgtga ggacggaacg acatgttcaa acgggaaatg
     2161 cgttgagtgc gaacctgtcg aactatgcgt tgaaaaaaaa tgttatgacg gaaatgtttt
     2221 ctgttatact gattgcggca acagaggcga acttgatgaa gtatgtgccc agaccgaagt
     2281 ttgtgaaggc ggcgcatgtc ttaagtgtga tgtaacagaa ccctgcgaaa caaagaaatg
     2341 tgttgattca gttgtttact gttatgacaa ctgcggaaag gaaggagaat tgtttcagga
     2401 atgtgaaagt tatgaaacct gtgaagacgg tgaatgtaat tgtactcctg actgcaatgg
     2461 aaagaattgc ggaacagatg gttgcggcgg aacatgcgga gaatgcaccg gaacagatga
     2521 atgcgtaagc ggaacatgcg gttccaaggt agatatggaa ggacctgaat ataaaggaga
     2581 tgttcttctt atcataaata caggaacgaa cgggagccag tcgagttttt caggaacact
     2641 tcctcttccg gttttaaaag ataatattgt ttcaaagagt gacggaactt cctttgtccg
     2701 taaatatttt gatcccggtg taaggatccc cggtgacctt gacctttcac aaaggcctgc
     2761 aaaaataata agatcggtgc cggcccccgc aatggatcct gttgcaggtg atactcagaa
     2821 tttctgggtg gcggatttcg gaagcggagg agacagggag ataaccgcga ctttacagca
     2881 cataagctca aaatgtgaag tatgggccga atatccaagc ataacaggca cgacaaaagc
     2941 aagacagata tgtgaagaat ttgacagtgt catatatccg atggtgacaa caaatttcca
     3001 tcctgaatcg gatataaact cggacggaag aatcgccttt ctcatagcag acctcggggg
     3061 tttcgccgca ggttatttca ctcctgcaga tttttattcc aaagaggagt attacggatc
     3121 aaacgaaaga gacatggtct atatcgaaca ggatatggac atttcccaga taagaaacac
     3181 cattgctcac gaatttcagc atctttgtta tgccaataga aattttctta ttgaacagga
     3241 caataattac agcgatcttg acgtcagatg gatagatgaa ggtctcgcaa cggcctcaga
     3301 gcatctgtat ggagggtccc agtcagactg ggtatatata ttcaacactt caccatataa
     3361 tgatccggtg gcgcagggga acggattttt taagtgggat tattctgaca ctaaccgcgt
     3421 ttattcagat tatgcactga cctatgcttt ttttcagtat ctgcggcttc agtcgccgga
     3481 ccaggatggc atatacaacg agataatcct taacaccaag aatgactggg acggagttca
     3541 ggatgtcatc aggagcagga tagacggcga catcaacttc tcagatttta tgaccgggtt
     3601 cagaattgca atgcttctta atgatgccgc aggaccttat ggattcatgg gggatacagg
     3661 actcaagttc acgccaaaat tctatacggg atcaggaaga aatgttgaag gaggcggtgc
     3721 actttatttt aagatttcgg gaagtttcac agagccggca gataaaggtg ccggaattat
     3781 ctatgtcggt atcaccacaa agtaaaggat ttaaacttta catcctggtt cctgtaaata
     3841 aaatgtttca ggatttttta tatggagatg ttcttgaata atcagatctt taaaataatt
     3901 tcagcagttt ttgtatcagg gcttcttttt tcctgtggcg gtccggaaag ttacaagaca
     3961 ccgatagtaa atctcggaga tatcattcag ggtgagtgca acccggtcgg tgaaggggat
     4021 cttcttgagg agagtttttc agcagaagtt gtcaacgggg atgttgtcgt ggaacattta
     4081 aatatactcg ttcctgaaag tacgatgatg ggaattattg ataccgacag caattcctct
     4141 cttgacggaa aattcaacta ttactatctt gaagcaagtg agcagttcat aagtgtcagg
     4201 gaaatattca gaaggtcatc ttcggaaaaa aactgctact atgatttgag aattaaaata
     4261 accaatgttt ccggcggaat atatgacctg atgcttttca atgaaaccgg caagcttaaa
     4321 ttctcaaaag agataagggt cagatgaact tgtcacctga aaaagtgatc gttctcaaat
     4381 tcggcgggtc atctttaaag gatgacgaaa cccttcaacg agcagtttcc ataataagtt
     4441 caagagtttt agatggttac aaagttgtgg cggttgtttc agcaagagga aagaccaccg
     4501 aggcattgtt gaaggatgca tctgtttttg cacctgccgg agatcccaga gaggttgaca
     4561 tgcttcttac aactggagaa agggcaagtg ccgcacttgt tgccatggct ttgaaatcaa
     4621 agggaattcc tgcaatttca tttaccggtt cacaagcagg tatcataaca gatgacaacc
     4681 acacaaatgc aagaataata gagataagac ccgacagagt aataaacgca ctgaatgaag
     4741 ggatgacagt aattgtcggc ggttttcagg gtgtaagttt taaaaaagag gttacaactt
     4801 tgggtcgcgg aggaagcgat acaacagcgg ttgcccttgc agcggcgctc aatgcggaaa
     4861 tctgtgaaat attcagcgat gttgatggcg tttactcagc agatcctaat cttgttgcgg
     4921 atgcaaagaa aattgatcaa ctcagctatc aggaaatgca ggaactctct gaggcaggag
     4981 ccaaagtcct tcattccaga gcagttgaat ttgcgaagga aaaagggata gagatccact
     5041 gcaaaagcac tttcgaacca gataatccgg gcacagtgat aagaggtttc gagacaaggg
     5101 agaaaattag aaaagccggt gtaacttatg aaaaaaatgt tatacttgta catgcttatg
     5161 agccggatgc atttgggctt ccaacaatta acaatgtatt gaaattctgc agggaaagaa
     5221 atgtttcagt aaagcagatc tcttttcata agggctataa ggaaagtatg gtgggcagtt
     5281 tcattctttc aaaaattgag aattattcct gcgatgcttt catttccgaa cttacaacaa
     5341 agtttgaagg cgcagttgaa atatttaaag aactttcggc agtttcaatt gtaggtgaag
     5401 ggataactga cagaccggac actttacttg aagcgataga acttctgaaa aacaatgaaa
     5461 tccaggtttc gggctttcat acaagttcat tcagaataag catgctcata aattttgcac
     5521 ttttagagaa agcggtcaaa ctgcttcatg aatactgcag ggcttaatct ttgttcctga
     5581 cacacatcgt gtatatctga ttctcatttt tatctttggg aagttccggc tttaatatcc
     5641 cgtacttaaa ttcaactacc catgcatttt gaattacatt tgcatttgtt gtagagctcc
     5701 agaagaaatc atctccaaga tcaggaaaaa ccgtctgaag ctcataatcg atctcataag
     5761 ttgccaagga atgaagttcg ttcacattgg gaagccgcca gtcagtcttt cctgcaaaac
     5821 tgagtgattc gcaatatgcc agagcatctt tccaaattct tgccgaataa tcggtcacct
     5881 tttgccattg aagtcctgtg agtttgtctt ctacgatggt gttctcatta atcacagtct
     5941 gagcgaatct tttgaatttt ctttcggtgt cgtaaatatg gtctgaactg acacagatgg
     6001 cagaattcat ttttgatgag ccgtcatcaa catagaactg actgctgcct gaaaaatcaa
     6061 catagtattt attatttcca ccgagttttg taagtgtcca gtatttctct tttttaagga
     6121 aagggaaata aaatccgtca acaagcggat cgatcgtttc atcaaaattc acaattgaaa
     6181 caagttcgtg aagataggga atgcgccagt ttgttctttc gccctgagaa gtgctgttaa
     6241 gaatttcaca aaaagcttgc gcatctgaaa agttgacagg attttcactt gctgttttat
     6301 accacagaag tttcgttcga ttgtcaaaaa caaaatattt atcaccgaat ttaatatttt
     6361 caaaaccgcg agttgaagaa tctgcataaa gtccgtcctg accgtaaaaa gtgcttgtca
     6421 cttcagtaca gtttgttgtt ccggtttcat tgaagcatct tttctgctga gtcgggacaa
     6481 ctttccagca gatctcacca aaattgcaga ggtcttcaat taaatcttcg tcggactggt
     6541 ctgaatcgtc tgactcgtcg ggaaattctg actggtcaga ctcgtcggga aattcggact
     6601 ggtcggacac ttcggactgg tcggacactt cggactggtc ggactcgtcg gactcgtcca
     6661 gctcttcaca aactccataa acacagacaa gatcgcctgt gcaggtgtcg ttgttgaagc
     6721 aggattctcc aactccgccc atgtgttcaa gtttgaatgt atcgcagccg gaaagtgcga
     6781 caaacaggat ggtgatcaat aatattctca tcttaccacc tccagctcag ataaacatga
     6841 gccccgccgg gaaccggaat aactgcagtc tttaccggag catcaatgaa aacatcagct
     6901 atctgcaacg cgacacctgt tgcaaaagcg actccgccga cagtatatga agttattgat
     6961 gtgtatttcc acgcgttgta ttttgacatg tcgccatcag tttttccttc ctgatagttc
     7021 tctttttcca caattgttct ccaggttgca attcctccaa aagcaaggac aactccgcct
     7081 gcaaccatta cacctattcc ggatttcatt aaaactgaca tctttttttt cggcttcttt
     7141 tcttttttaa ctattttttc atcttttgcc gcatctttaa ttccgaaaag ctctcttaca
     7201 gccggggcga tcatttcaac gatcatgttc acatcgccct ttaccctgtt ttcgtatctt
     7261 gagatcactt caacatttct gacatttagc cgctgaaggg tgagaatcca gtaatcgccc
     7321 atttttgaaa ttgtgccggt tattgattca ggagctccga gtgcagctcc gatctctgcc
     7381 acacaagccg tatcttctgc tccgcatgac attttcatct cttcacctac gatcagagct
     7441 ttcatttcat tctctccgac agtcttcctt cccgaaaccc tgcccacctc agcagtgact
     7501 attccgctta ttgcgtctgc ctctttatcg gtcactcccg ttttcggctc tatccgccag
     7561 acaactatcg aatcattttc tacatcattg ggaaatagtt ccaaagcaaa aagaaaagaa
     7621 actgcaaaca tcagacagcg ttttaatacc atgccacatc tcctgagaat gaaactttta
     7681 taaatatatc taaacacgga aataaatcaa aaaacacaat gaaattaatc ttttctgatg
     7741 tttggcaaga tacttgtcaa tttaaattga ctctgtaaaa aaactgttta ttttggaatg
     7801 actatactta ttgtaagttt gtcaaaaaac tttggaagat gaaaagctgt gtcgacaata
     7861 gttaatatta ttaaaaactt aacctgctaa aaggaggcaa aaaatgaatt atctggatca
     7921 acaattcgag aattttaaga atctgccgat tgaaaaaaag atcgaacttt ttgaaatgga
     7981 cggcaaactg tttgacatga taatttctca acagctttcc agggaagtgc ttgaaacgct
     8041 ctataatctt actaatcatc tcagaagaat gggaaaactt aaggaagggc ttgattttct
     8101 tgcaacactt ctgagccata aaagggcaat gctctatttt gaacagccct catcaagaac
     8161 atttctctcc tttcttaatg cctgccatat tctcggtctt aaaacttctg aaattagaga
     8221 cacatctgtt tcttctgaag ttaaaggtga gagttacttt gattcgatca gaactttctc
     8281 ttcttacaca gaccttgtaa taatgagatc aaaaaataaa gatgcggcag aaaaggcggc
     8341 atggcttctc aacacatatt caaaccgtcc ggttccgata atcaacggcg gttcaggagc
     8401 agaccagcat ccgacacagg cacttctgga tatttatacg cttatgagaa gtttcgccaa
     8461 tcagggtggt cttgacggaa agaccgttat gttctgcggt gacttgagaa gaggccgtac
     8521 cgcccgttca cttgcatacc ttcttgaaag attcaatgat gttaaactga tcttctgtgc
     8581 ccccaaagag ttccagatcc ttgatgatat acttgaatca cttgacagaa agggtgtcaa
     8641 atacgaaata atcactgaat cggtaagaag cggtcttgac agagctgatg ctatctacat
     8701 gacccgtatt caggatgaac atgatcttgt tgccggagaa agttcaacga ttgacatatc
     8761 aaaattcaaa ctttcaggcg aagaccttaa atacatgaaa ccgggtgccg caatacttca
     8821 cccgttcccc cgccgtgacg agatcgcaac agaaatcgac agtgacccgc gtgcaaaata
     8881 ctggagacag gggagaaacg gcatgtggac aagggttgct ctgatcgcct atgtctttaa
     8941 tcttgaaaaa cagatcatgg aatttgatgt ttccagggtg gatcatctga gatagaaatt
     9001 ccttcaaaac ctctacgaaa taatttccag aatgttgtga caataacaat gggaaaagat
     9061 ttctgcgttt aagttgtttt aaatcctttt ttcatccatt tggaagcaag aagaagtgca
     9121 aaggggctga tcattgcgat caggccgtaa atgaaccaca tggtttcggt gttcatttgt
     9181 gacggggcgg tgtctgcggg gcagtatttc atgaggaacc agccggaata tattgctgtg
     9241 acgacttttg tgagaaacca cgggaactgt ccgattccca tatagattcc ggtcattcct
     9301 ttgggtgcga tctgtgcgac ccactgaagg aatctcggct gccagatcgc ttcaccgatg
     9361 gtcattatga caagatatgt cataagcgtt cccatgctcg gtccaagtgc gagaatgaaa
     9421 gttggagccg ccatgatgaa tgttccgatt atcatcattt tataggtgtc tttctttgct
     9481 gtcatggctg tgaccatggg ggtgagaata aagataagta tcggatttaa attgacaaaa
     9541 aactcaaagt tgtcgccgac aaacccatca aaggccctgc ttgtatattg cggcagagta
     9601 agccagttgt gtgcaaaaag cgtttgaaca gggatcagaa caaatatgaa gaacatgaac
     9661 cttgcatcgg ttatcgggaa gtttttgaga tagaacttca ttttttctgt tgctgacatt
     9721 ttctcttttt cttcgtcggt ttcctctttt tcaaggagtg ttttatccgg ttcatcggct
     9781 cttgcctgat caattgcatc tttgagagct tttttagtta tgaagatcgc gacaacggca
     9841 agacctaaaa cggtaagtat cgaataaacc cagaaaactc cgcttattcc gaaacttttt
     9901 ctaaccggcg gagaaataag ccccggcaga aatccgccaa ggttcatcaa agcgtaaagc
     9961 attgcatatc ccattgccga agttctgctg tttgtcagca tttttacacc cgaatatgcc
    10021 gccggcatca atgtcattca actttatgta ttcattgaag aataatgcca gaagcccaac
    10081 gatcccgaaa tagacaagac cctcgatcac ataagaaata ttaagcccaa gtaatggtct
    10141 tgaagtcttt ccaagatcaa ggaacggctg gataagttct ttcaccatcg aatctttttt
    10201 ctgctctgtg ttagtttcca ttcttttact ccgcaaaaat taaaaagcca catcatttta
    10261 aaaatttttc agaaaaaagc acttattaag ttgacagatc actcataaac tgatcaaaca
    10321 cgccttattt tgactctgta ctctctttca agtatatatt acagttttgt tgatttgtgt
    10381 gatacgggga taaattg
//
LOCUS       DAOOYB010000095        10366 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_6944_length_10366_cov_9.280574, whole genome shotgun sequence.
ACCESSION   DAOOYB010000095 DAOOYB010000000
VERSION     DAOOYB010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10366
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6944_length_10366_cov_9.280574"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            537..1925
                     /locus_tag="PL056_07070"
     CDS             537..1925
                     /locus_tag="PL056_07070"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HPM46813.1"
                     /translation="MPFYKISLFLFIYVLLSSFSDNENDPFASFSKSFYSYYFSEDFA
                     SDNIKKEFPFIRKQKESSDPRLESYADKPNIIILAIESFNENFIDESFKNNNKLTPFF
                     NELKKKGTYVERFYGNSIQTSKGHFATLFSLIPSYKAKVFTSFANVKFKSLPDILKDY
                     GYRTYFLMAHQHPKFDNTERFLTKNGFDAVESLFPFLTEKEINDIWGWGMEDSLFYRK
                     LFNYLDHRKVENNKNEPFFIFAATIMNHAKFDVPVDKKIVVRESYSLLENYYNSVRLV
                     DEYLKVFFEEMEKRDYLKNSIIIITSDHSYPLGQHGLNHNETSYYEEFFRIPFLIIWN
                     NHITPAVISDDVYSQMDIAPTILDLINITPPKHHFLGRSIFKKDDNLNNAFLVQPYNG
                     GYLGVVQLPYKYIRSVKTDKEILYNIIKDPGENKNIIDSENTEIVEKFRELVQKSYMN
                     YYLLKKNKIWDE"
     gene            2062..3414
                     /locus_tag="PL056_07075"
     CDS             2062..3414
                     /locus_tag="PL056_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46814.1"
                     /translation="MLNCRNLGGGFYWDEAVYANISEHLLYSDYYNPAYRVFTRHPPL
                     YFLFLSLISNIPFQKEIVFRTFSVIFSIIGTGFLYYLFLSMKRFKLGVVFLALLVMNT
                     LFQQYSQSATMYAMLFMFFSVFIAGVVEKNENLELVGMIGGIYTHYLMFVSLLIVVLL
                     DYFLNKKRISPGLKRKLIIIAVSYLPWLLFFFPGFFYHAKKYSEFTAHINLYQFLWTF
                     GITGLIAISFWAYRIFKERAKCLENRDNEIRFVVLATIFIIVHLALYLFGTPFLRYLF
                     FILPLVYAVTLLSVARFLEHKKYSSILKKNGLFFILLLMMFVPNLKMLGVFPYSFSYH
                     DNHDSIYNSYWDEIINDVHGSTVSVENVRSFVYYANKKYPGKYNTIFPWDALGVVELK
                     YNPNNNENNIKNLEKISTTFVCIDSVGRYKSEMIAHLNNSGYKECKRYGSWILLKNEI
                     "
     gene            3459..4436
                     /locus_tag="PL056_07080"
     CDS             3459..4436
                     /locus_tag="PL056_07080"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HPM46815.1"
                     /translation="MLAKIKDSKKYFYNGMKILFFILSIMLLYFLLRTIGFIKVRDSF
                     ISVGISGAVILVLMGFVENILDSASLKYSSLKSVPLYKIHAINSLGGIMNSVFPWETG
                     EVVKGLMIKKDASLNDSVFGVVLYNYIFKISKPVAILFIIIVSLMFQVEADTAYILLI
                     FLACFVSFIPYIFITLLIRYSAVTKFAKIIEKLLNKDLSKWKEKIEKFEEAIKKFRKD
                     KNKSFFFVFTLQFFARMTSTVTFLIAAYLLGDIKTIFPLLILANAAIYLANYVVLMLP
                     AKLGVTESTSFLIFSMLGLDGGLGVMIVLVLRIKALFTQSISCLLLPFI"
     gene            4484..5137
                     /locus_tag="PL056_07085"
     CDS             4484..5137
                     /locus_tag="PL056_07085"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HPM46816.1"
                     /translation="MKKYSFPKAILFDLDGTLINTMEGFADLAGELIERDFGVDFVTA
                     RRNYITTSGIPFKHQLEVMFPGDSRNYDVQKEFEERKQNVFFNTIPDNDTIDAVRRLK
                     DMNIKVIVSSNNYHDFVLKYFSENSSLKFDMILGYKDGFAKGKDHILHVVEAFNISRK
                     DMIFVGDSLHDGEVADELEIPFIGRIAIRTREEFLSKFTDILCVDRLGEISDLFTDY"
     gene            complement(5139..6293)
                     /locus_tag="PL056_07090"
     CDS             complement(5139..6293)
                     /locus_tag="PL056_07090"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HPM46817.1"
                     /translation="MEIKKTKFSIVIYAKTNSKHDVDQKLLGLSLLKRQILTAENLDP
                     ENVVVVKNENDTIPSGRILFINAAYFVHPLALTEIAAGDSFFIEEGGYLAFLDSKTAA
                     NEIKDFLKNGIFKSENTTPPSPGYRVLVDSPEKLKVLSKELFNAMRKPVDGLVARTLN
                     KAVSFFLTEYVWLPLRFTPNMVTFITVCIGVLSGIFCAGGTYTYLVIGTFLSHFASIL
                     DGCDGEVARLTFKTTTIGKWFDSVGDSIVNTSLMIGLGYGLEPYFGTLPIIIGWSTTA
                     VSWIHNFVSYYDVIVVRKTTDIFAFTWWFEKKDRVRKIDLSKPVYGTISFLDLLKYFS
                     RRDFYLFIYFVLAVVGPTAIMFGYIATAIFLVPSFFLTIAHIYFGIIKKI"
     gene            6439..8325
                     /locus_tag="PL056_07095"
     CDS             6439..8325
                     /locus_tag="PL056_07095"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HPM46818.1"
                     /translation="MQIVILAAGLGSRLGKGVPKALVEVAGKTMLEYQMKWVKMFNPE
                     KVIVVSGYLHDDMKLFCEQKFPYAVIVENKRYREQNLYSLLAAKDFLNDDTLIMNVDH
                     IYPDAFAQRVAPQLKDVENYAIFADTERSLTDDDMKVFINSDGYVEKISKKLTEWNAG
                     YIGMTFIKKDFWKKYLSAASTVAKIQKEKAVVEMVIQELVDGKNFPEIINSDKMTWYE
                     IDTPEELKYAENCLPSCIRVALFKQKTDTPDFAGVNVPERWQRAIDKLDISFKRRIEE
                     VNSEDIQECFKVPSQKTVLIFTDWIVDDVTVESALESVYSEVSDDEFVSFTGKNGVAP
                     LFIIGRAAQQKLSVWEGWKDENVSAAKLVNFAKTLDKVKAIEKTLEKGYWGNIKNGED
                     VSKVEWEYLKFLQFRPGGVVAKYLNRPVSIRITRNILKYKFMTPNFVTVADFFIGLLG
                     LACFLIPGYLGAVLGGVIIHFNSIVDGVDGEIARMRHQGSSLGAWLDSICDETLGALL
                     YLAIGYHLYISGGSFLFLAAGIITAVASYTYALVHWHCKYKHGLGFYYWWECYKPRKE
                     VQRSTSLVSYLKKLLWRDSILLIVMIAAVFNFLDVFLVIAMFPALVNFILIAIHIFIK
                     KARW"
     gene            8319..9278
                     /locus_tag="PL056_07100"
     CDS             8319..9278
                     /locus_tag="PL056_07100"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HPM46819.1"
                     /translation="MVKKNLSGKIVKILIFIFSLTVLFFLLRHIGFEKVGESFVKVGI
                     PGAVILIILGLIENGSDAWALSFAIPGKISFIKVFSSNCIGALTNLIIPWEAGEVVKV
                     GLIKKETGASSAIKGIVLWNYIFKLSKAYALITILIISFIAGHDYRTDHFTIVFGAAL
                     IGFLPYFGMMILIRSNISVKLAKLLKLLGRKNSDDLLKKAEEMDSSLKKFRKERPRDY
                     RAVLFIQFFARFVSWATFLACAYLAGFDYSFSMLSLAYCAIALSGYIVSFIPAKIGVG
                     EGAGFLIFSFLGLDGGAGLLITFILRIKAIIAMSIASFLNVVK"
     gene            complement(9288..>10366)
                     /locus_tag="PL056_07105"
     CDS             complement(9288..>10366)
                     /locus_tag="PL056_07105"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HPM46820.1"
                     /translation="EMEYNFDKLTFPGKYDRSSITMQYLDAVKKNKADVISTGLPSFI
                     LTDSAEETEDATRTLSEMMHYPDKRTVALKDVLSAYTFLPPDQEKKLGLIKNIRQLIE
                     RKINLFDEKTIERYNSDFKSYLAIESQMDKDLLPEWIKDKMSLKDGSFDKFIIIALGG
                     NKSNVEDVLTIRNEYGTVHGKIKDYKILGSYMLLGEIKTVIDRHVPLATLFAFLAVFA
                     VLVILYRSFRNAFIVFLPLSAGILWMIFVAVMSGIKFNIFNMIVIPTVIGTGIDSAIH
                     IYHRYRTSSNESMVENLKNTGGAVFFSSLTTLVGFGSVAFAAHKGLQSIGIMASIGII
                     TVTLVNLVFFPVIIVFIQERFQKK"
BASE COUNT         3196 a         1594 c         2009 g         3567 t
ORIGIN      
        1 gatactgcgg tcagtggccg tggaaaagta ggtcgtcgcc tcattttttt tgcctttttt
       61 taatttgcca aaaataagat ggaaattaaa tgtaccgatc tttaattaaa aagaatacag
      121 tgtatttgtt gtttctgttt tttttctcat ttttgataaa tacatttttt tattacaacc
      181 gctcatcagt agtaatctct ttttcagttg attttttatt taatatactt tctgatctta
      241 ctgcattgac cattttgctt tggttgattt tgaagatgac ttcccgcagt ggtattgttt
      301 tacaaagaat tgtgtggata ctttatttat ttttgtataa tctttttagc tcttaccggt
      361 ataaatcgaa gattaatttt gattattctg tgtgggcaga caatttttat gagagttttt
      421 attttgaatc tctggaacta atatttcaaa gcatttcaat taagatcctg ttttttggaa
      481 tgattccttt aatttttctt tttttacaca aagaaattaa agaatctgag aaaaggttgc
      541 ctttttacaa aatttctttg tttttattta tatatgttct gttgagcagt ttttcagata
      601 atgaaaatga tccttttgct tcattctcaa aatcttttta cagttattat ttcagtgaag
      661 attttgcttc agataatata aagaaagaat ttccttttat ccgaaagcaa aaagagtctt
      721 ctgaccccag gctagaatct tatgctgata agcctaacat tataatatta gcgattgaat
      781 catttaacga gaattttatt gatgaaagtt ttaaaaacaa caacaaattg acgccttttt
      841 ttaatgaact caaaaagaaa ggaacctatg tcgaaagatt ctatggcaac tctattcaga
      901 catcaaaagg tcattttgca actctttttt ctcttatacc aagctataaa gcaaaggttt
      961 tcacctcttt tgctaatgtg aaatttaaat ctcttccgga tattttaaaa gattatggtt
     1021 acagaacata ttttttaatg gctcatcaac atccgaaatt tgacaatact gaaaggtttt
     1081 tgacgaaaaa cggttttgat gcagttgaat cgctttttcc ctttttgacc gaaaaagaaa
     1141 taaatgatat ttggggttgg ggtatggaag attcgctttt ttataggaag ctttttaatt
     1201 accttgatca tagaaaagtt gaaaataata aaaatgagcc attttttata tttgctgcca
     1261 caataatgaa tcatgctaaa tttgatgttc cggttgataa aaaaattgtt gtcagagaat
     1321 cttactccct tcttgaaaat tactataatt cagtaagact tgttgatgaa tatttgaagg
     1381 tgttttttga agagatggaa aaacgggatt atttaaaaaa ctccatcata attataactt
     1441 ctgatcattc ttatccgctt ggacaacacg gtttaaatca taatgaaaca tcttattacg
     1501 aagagttttt cagaatacct tttttaatta tatggaataa tcacattaca cctgccgtta
     1561 taagtgatga tgtttattct caaatggaca ttgctccgac tattcttgat ctaattaaca
     1621 ttacacctcc caaacatcat tttctcggga gatctatttt taaaaaggac gataatttga
     1681 ataatgcttt tcttgttcaa ccgtacaatg gcggatatct cggtgttgtt cagcttcctt
     1741 acaaatatat cagatctgta aagacagata aggaaattct ttataacatc ataaaagatc
     1801 ctggagaaaa caaaaatata attgattctg aaaacacaga aatagttgaa aaattcagag
     1861 aattggttca gaaaagttat atgaactatt atttgttgaa gaaaaacaaa atctgggacg
     1921 aatgagatat tttttgacta aaaattttgt gtaagtataa aaaagctgat tttaattatt
     1981 taataaatta ggtatagtgt gattcaaaac aagggatttt ataaatggtt gccttttatt
     2041 attttttccg gcctgttctt tttgcttaat tgcagaaatc ttggtggggg tttttattgg
     2101 gatgaagcgg tttatgctaa tatttcagaa catcttttat attccgatta ctataatcct
     2161 gcttacaggg ttttcacaag acatcctcca ctttactttt tgtttttatc tttaatttct
     2221 aatattcctt ttcagaaaga gattgttttc aggacattca gtgttatttt ttcaataatt
     2281 ggcacaggat ttctatatta tcttttttta agtatgaaaa gattcaaact aggggttgtt
     2341 tttctggcac ttttggtaat gaacaccctt tttcaacaat acagccaatc tgccacaatg
     2401 tatgctatgc tgtttatgtt cttttctgtt ttcatcgcag gtgttgtaga aaaaaatgag
     2461 aatctcgaac tggttgggat gattggcgga atatacacac attatttaat gtttgtttca
     2521 ctcttaattg ttgttttatt agattatttt ttgaacaaaa aaaggatatc tcccggttta
     2581 aagagaaaac taattattat tgctgtttct tatcttccgt ggttactgtt ctttttcccc
     2641 gggttttttt atcatgcaaa gaaatacagt gaatttacag ctcatatcaa tttatatcag
     2701 tttttgtgga cattcggaat aactgggctt attgctattt ctttctgggc atacaggatt
     2761 tttaaagaaa gagcgaagtg tctggaaaat agagataacg agatacggtt tgtcgtttta
     2821 gctacaattt ttattatagt acatttagcg ttatacttgt ttggaacgcc ttttttgaga
     2881 tacttgtttt ttattcttcc tcttgtatat gcagtaactc tattgagtgt tgcaagattt
     2941 ttagaacata agaaatatag ctctatcttg aaaaagaacg gacttttttt tattttactg
     3001 ctcatgatgt ttgttcccaa tttaaagatg cttggggttt ttccgtattc attcagttat
     3061 catgataatc acgattcaat atataattca tattgggatg aaataatcaa cgatgttcat
     3121 ggttcaaccg tatctgttga aaatgtcaga agttttgttt actatgcaaa caaaaaatat
     3181 cctggtaaat ataacaccat ttttccttgg gatgctcttg gtgttgttga gcttaaatat
     3241 aatccgaaca acaatgaaaa caatattaag aatcttgaga aaatttcaac aacatttgtt
     3301 tgtattgatt cagtaggcag atataaatct gaaatgatcg ctcatttgaa taattcagga
     3361 tataaggagt gtaaacgata cggatcgtgg attcttttaa aaaatgaaat atgatagatc
     3421 ggacaacggt tgtttcaaaa catttggaga tatgtcaagt gttagctaaa atcaaagatt
     3481 ctaaaaaata tttttataat ggaatgaaaa tactgttttt tattctttca ataatgctgc
     3541 tctatttcct cttacgcact ataggtttca taaaagtaag agactctttt ataagtgtcg
     3601 gaattagcgg agctgttatt cttgttttaa tgggatttgt agagaatatt cttgattcag
     3661 cctctttgaa atattcaagt cttaaatctg ttccactcta taagatccat gcaataaaca
     3721 gtctgggtgg aataatgaat tctgtttttc catgggaaac aggagaagtt gttaaaggtt
     3781 taatgataaa aaaagatgct tctttaaatg attctgtttt tggcgtagtt ttatataatt
     3841 atatctttaa gatatctaaa ccggtagcga ttctatttat tataatagta tctcttatgt
     3901 ttcaggtaga ggctgatacg gcttatattc tgcttatttt tcttgcgtgt tttgtttctt
     3961 ttattcccta tattttcatc acattgctaa tcagatattc ggctgttaca aagtttgcaa
     4021 aaatcataga gaaactatta aataaagatt tatcaaagtg gaaagaaaaa attgaaaagt
     4081 ttgaagaagc tataaaaaag tttaggaagg ataagaataa aagcttcttt tttgttttta
     4141 cgctacagtt ttttgcaaga atgacatcta cggttacttt tcttattgct gcttatttgc
     4201 ttggtgatat caaaactata tttccactgc ttatactggc taatgcggca atctatctgg
     4261 cgaactacgt tgtattgatg ttaccagcga aacttggtgt tacagagagc acttcctttt
     4321 tgattttttc tatgctggga cttgatggag gcttaggagt tatgatagtt cttgttttgc
     4381 ggataaaagc tctttttacg cagtcaattt catgtctgct attacctttt atctaattat
     4441 gaagagaagt ttgactgtga aactatttaa ataaaggtga aaaatgaaaa agtattcatt
     4501 tcctaaggca atactgtttg atcttgatgg aacactgata aacacaatgg aagggtttgc
     4561 cgatcttgca ggagagctga ttgaaagaga tttcggtgta gattttgtta cggccagaag
     4621 aaactatatt acgacgagcg gtattccatt taagcaccag cttgaggtga tgtttcccgg
     4681 tgattcgaga aactacgatg ttcagaaaga gttcgaagaa aggaagcaga atgttttttt
     4741 taatactatt cctgataacg atactattga cgctgtaaga cgcttgaaag atatgaatat
     4801 aaaagtgata gtttcttcaa acaattatca tgattttgtt ttgaaatact ttagtgaaaa
     4861 cagtagtttg aagttcgata tgatacttgg ttataaagat gggtttgcaa agggaaaaga
     4921 tcatattctg catgttgtag aagcgtttaa tatttcaaga aaggatatga tatttgtagg
     4981 agattctctt catgatggtg aagttgcaga tgaattggaa attcctttta tcggaagaat
     5041 agcgatcaga acaagagaag agttcctatc aaaatttact gatattctct gtgttgacag
     5101 gcttggagag atatcggatt tattcacgga ttattaattc agattttttt gattattcca
     5161 aaataaatat gggcgattgt tagaaaaaat gatggtacaa gaaatattgc ggttgcgata
     5221 tatccaaaca ttattgccgt aggccctact acagccagaa caaaatatat gaaaaggtaa
     5281 aaatctcttc ttgaaaaata tttcagaaga tctagaaaac ttatagttcc gtaaaccggt
     5341 ttggaaagat ctatttttct gacacggtct tttttctcaa accaccaggt gaaagcgaaa
     5401 atatcggttg tttttctcac gacaattaca tcgtaatagc tcacaaaatt atgaatccag
     5461 ctaactgccg ttgttgacca gccgattata attggaagtg tgccgaaata tggttccaga
     5521 ccatagccca gaccgatcat cagagacgtg tttacaatag aatctccaac gctgtcgaac
     5581 catttaccta tggtggtggt tttaaatgta agccttgcaa cttcaccgtc gcatccgtca
     5641 agaattgaag caaaatggga aaggaaagtt ccaatcacaa gatatgtata tgttccgccg
     5701 gcacagaaaa ttcctgaaag aactccaata catacggtaa taaaagtaac catgttaggt
     5761 gtgaagcgta aaggaagcca cacgtattct gtaagaaaaa atgaaacagc tttgttcaga
     5821 gttctagcga caagaccgtc aaccggtttc cgcatagcgt tgaaaagttc tttggacagc
     5881 acctttagtt tttcaggaga atctaccaga actctgtatc cggggctcgg aggtgttgta
     5941 ttttcagatt taaaaatccc gtttttaagg aaatctttaa tttcatttgc agctgttttg
     6001 ctgtcaagaa aagccagata tccgccttcc tcgataaaaa aagaatctcc ggctgcaatt
     6061 tctgtcaatg caagaggatg aacaaaatat gcggcattaa tgaaaagaat tctgccagaa
     6121 ggtattgtat cgttttcatt tttaacaacg acaacatttt ccggatcaag attttctgct
     6181 gtcagaatct gtcttttaag aagagaaagc ccaaggagtt tctggtcaac atcatgtttt
     6241 gaatttgttt tagcataaat tactattgag aattttgttt tttttatttc catttgacgc
     6301 tcgtttaaaa aaatcaaatt tgcggaaatg attatagtct tgcagagtat tattgcaaat
     6361 tttgcggagc ttttctttac ttaggtgtat ttggaaataa aatccaatgg tttgttaatt
     6421 atttgaatga ggataatgat gcagattgta attcttgcgg ctggtctagg ttcaagactg
     6481 ggaaagggtg ttccgaaagc actggttgaa gtagccggga aaactatgct tgaatatcag
     6541 atgaaatggg tgaaaatgtt taatcctgaa aaagtaattg ttgtttcagg gtatctccat
     6601 gatgatatga aattattctg tgaacagaag ttcccttacg cagtaattgt tgaaaacaaa
     6661 cgttacaggg agcagaatct atactctctt cttgcagcaa aggattttct taatgatgac
     6721 accctgataa tgaacgttga tcatatttat cctgatgctt ttgcacaaag ggtcgcacct
     6781 cagttaaagg acgttgagaa ctatgcaatt tttgcagata cagaaaggtc attgacagat
     6841 gacgatatga aagtttttat aaactcagac ggatatgttg aaaagatatc aaagaagctg
     6901 acagaatgga atgcaggata tatcggaatg acctttatta aaaaggattt ctggaaaaaa
     6961 tatctgagtg ccgcttcaac tgttgcaaag attcaaaaag aaaaagcagt tgttgaaatg
     7021 gtgatacagg agcttgtgga cggaaaaaat tttcctgaaa taatcaattc agataaaatg
     7081 acatggtatg agatagatac tcctgaagag ttgaaatatg ctgagaattg tcttccttcc
     7141 tgtataagag tcgctctttt taagcagaaa acggatacac ctgattttgc gggtgtaaat
     7201 gttcctgaaa ggtggcaaag agcgatagat aagctcgata tttcttttaa gcgaaggatt
     7261 gaagaggtca attctgaaga tattcaggaa tgttttaaag tgccgtccca gaaaacagtg
     7321 cttattttta cggattggat agttgacgat gtaacggttg aatctgcact ggagtctgta
     7381 tattctgaag tttcggatga cgagtttgtt tcttttaccg gtaaaaacgg ggtggctccg
     7441 ttatttatta tcggtcgtgc ggctcaacag aaattgtctg tatgggaagg atggaaagac
     7501 gaaaatgttt ctgccgctaa acttgttaat tttgcaaaaa cgctcgataa agtaaaagca
     7561 atagaaaaaa cacttgagaa aggatactgg ggaaacataa agaacggcga agatgtgtca
     7621 aaagttgagt gggaatattt gaaattcctc cagttcagac cgggaggagt tgtcgcaaaa
     7681 tatttaaaca gaccggtttc aataagaata accaggaata tcctgaaata taagttcatg
     7741 accccgaatt ttgtaacggt tgcagatttt ttcatcggac ttttgggtct tgcatgtttt
     7801 cttattcccg gttatctcgg agcagtgctc ggcggagtga taattcactt taactctata
     7861 gttgacggag ttgacggaga gatcgcaaga atgagacatc agggatcttc tctgggtgcg
     7921 tggcttgatt cgatatgtga cgaaacgctg ggagcacttt tatatcttgc aattggctat
     7981 catctttata tttcaggagg gtcattcctt tttctggcag ccggaataat aacagcagtc
     8041 gcttcttata catatgcact ggtacactgg cattgcaaat ataaacacgg actcggattc
     8101 tattactggt gggagtgtta taaacccaga aaagaggtgc aaagatcaac atctttagta
     8161 agttatctta aaaaacttct ctggagagat tcaattctgc tgatagtaat gattgcggcc
     8221 gtttttaatt ttcttgatgt ctttcttgtt attgcaatgt ttcccgccct tgttaatttt
     8281 atacttatcg ctattcacat atttataaaa aaggcaagat ggtaaagaaa aatctgtccg
     8341 gcaaaattgt aaagatcctg attttcattt tttcgttgac ggtactcttt ttccttctta
     8401 gacacatcgg gtttgaaaaa gtaggcgaat cgtttgttaa agtcggaatt cccggggctg
     8461 tgatcctgat aatactcggt ctgatagaga acggttctga tgcatgggca ctttcttttg
     8521 cgattccggg aaaaatatct ttcattaaag tcttttcctc aaactgtatc ggtgcgctga
     8581 caaaccttat aattccgtgg gaagcagggg aagttgtgaa ggttggtctt ataaaaaagg
     8641 aaacaggggc gtcttcagcc ataaaaggta tcgtactttg gaattatatt ttcaaactgt
     8701 caaaggcgta tgctctcata actattctta tcatatcttt catcgcggga cacgattaca
     8761 ggactgatca ttttactatt gtctttggag ctgccctgat agggtttctt ccttattttg
     8821 gaatgatgat cctgataaga tcaaatattt ctgttaagct ggctaagttg ttaaaattgc
     8881 tgggtagaaa aaacagtgat gatctgttga aaaaagctga ggaaatggac agcagcttga
     8941 aaaaattccg caaggaaagg cctcgggact acagggctgt gctatttatt cagttctttg
     9001 ccagatttgt ttcgtgggca acatttcttg cttgtgcata tcttgcggga tttgactact
     9061 ctttttcaat gctctctctg gcatattgtg caatagcttt aagcggctac attgtttcct
     9121 tcattcctgc taagatcgga gtaggtgaag gtgccggctt tctgatattt tcttttctgg
     9181 gacttgacgg aggagcgggg ctgctgatca ctttcattct gcggataaaa gctataatag
     9241 cgatgagcat tgcttctttt ttaaatgtcg taaagtagcg aaatcactta ttttttctga
     9301 aaccgctcct gaataaatac gatgataaca ggaaagaaaa cgagatttac aagagtaact
     9361 gtaattatgc cgatacttgc cattattccg atggattgaa ggcccttgtg ggcagcaaaa
     9421 gcgacacttc cgaatcctac aagggtagtg agtgatgaaa aaaagacagc tccccctgtg
     9481 tttttaaggt tttcaaccat tgattcattt gaagatgtgc ggtaacggtg ataaatgtga
     9541 attgcagaat cgatacctgt gccgattacg gtaggtatga ctatcatatt gaaaatattg
     9601 aactttattc ctgacatgac tgcgacaaat atcatccaga gaattcctgc ggacaatgga
     9661 agaaatacaa tgaaggcgtt tctgaaagag cgatagagga tcacaaggac tgcaaaaaca
     9721 gcaagaaaag cgaacagcgt agcaagagga acatgtctgt ctattacagt tttaatttca
     9781 cctaaaagca tatatgatcc gagtattttg tagtctttga tctttccgtg tacggtcccg
     9841 tattcgtttc tgatggttaa aacatcttcg acatttgact tgttaccccc cagagcaatg
     9901 ataatgaatt tgtcgaacga gccgtctttc agtgacattt tatcttttat ccactcgggg
     9961 agaagatctt tgtccatttg agattctatc gcaaggtagg atttaaaatc agagttatat
    10021 ctttcaatag ttttttcatc aaacagattt atctttcttt ctatcaactg tcttatgttt
    10081 ttaattagtc ccagtttttt ttcctgatcg ggaggaagaa aagtatatgc agacagaaca
    10141 tctttaagag ccaccgtccg cttgtcagga taatgcatca tttctgaaag agttcgtgtc
    10201 gcatcttcgg tttcctctgc tgaatcagtg aggatgaacg agggaagtcc tgtcgagata
    10261 acatcggctt tgtttttctt aacagcatcc agatattgca tagttattga tgacctgtca
    10321 tatttgccgg ggaaagtcag cttatcgaaa ttgtattcca tctcgc
//
LOCUS       DAOOYB010000096        10257 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_7054_length_10257_cov_12.602137, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000096 DAOOYB010000000
VERSION     DAOOYB010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10257
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7054_length_10257_cov_12.602137"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /locus_tag="PL056_07110"
     CDS             complement(<1..740)
                     /locus_tag="PL056_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46821.1"
                     /translation="MRHFVVSVMLAVFAFSCGDSVATKSKPKNDNDASVSDNDSDQTV
                     SDDENVADDNQTVKDDEAVKDDISVPDEDSVISDTCGNTSIEWPEVCDGGTMDCVDID
                     DTKYSSGKAKCKDNCSGWDTATCVEIEPENCMNGDRRCYGAYQYQICAGGVWGEPATC
                     SKSGTCYGDGLCSNDCYPHGSYSCYNGQVYWYDTCGEMEEIKENCGTSGYVGSKYCID
                     DEVYQDYKTVYCDYQSCDSETESVYIEY"
     gene            887..1915
                     /gene="purM"
                     /locus_tag="PL056_07115"
     CDS             887..1915
                     /gene="purM"
                     /locus_tag="PL056_07115"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017032638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HPM46822.1"
                     /translation="MSELNYKNAGVDIVEGAKMVEKIKPLVKRTMRPEVLAGIGGFSS
                     LVSIPEGIKNPVLVSGTDGVGTKLKFAFLSGIHNTIGIDLVAMCVNDVIVGGAEPLFF
                     LDYFATGKLSAEVGAEVVAGIAEGCFQAGCALVGGETAEMPDFYESGEYDLAGFCVGV
                     VDREKIIDGSKIKEGDCIIGIPSTGVHSNGFSLVRKIVFNKMGLSTGDMIDELGLTVA
                     QEFLKPTRIYVKEVLELIKKFKISGMVHITGGGFYENIPRVIPEGLGALIDGKFPVLP
                     VFKWMKNCAGLTQREMFTTFNCGTGFMLIVPEDQVEELLKDSDRYYVGRIVRTDEVER
                     VVIPGNHF"
     gene            1926..2948
                     /locus_tag="PL056_07120"
     CDS             1926..2948
                     /locus_tag="PL056_07120"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46823.1"
                     /translation="MRKIFFLLTLPAFILLSAGCSSAERTQNRKDQENLKLLEQNVES
                     ADSFFIEGKDSFIKNEYGSALESFQKSSYSQALFYIGLTLSELGKKEDAKEVFMDCAA
                     KNILPAESYYNLSMIAYDLGDINLSKEFAIKSIGLKPDHTGALFFAGNLFYVESNMAE
                     ALNYYNKAIATDPSSVDLWEAVFSVYLQTEEFEKGWAIRDKIDRNNTGTVLNVLKVAE
                     ITGNFKEGADFPKKELLEDSAVNQQVRILLTRAGEIKKAAEMAQKEMEKNKKPYLIID
                     RMTGDNGSYVIVLKESSVFLVCSAKSEIFIPVEISSGIVKIDGRNSVKSHDVSKEAVS
                     VCSEQK"
     gene            3035..3760
                     /locus_tag="PL056_07125"
     CDS             3035..3760
                     /locus_tag="PL056_07125"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HPM46824.1"
                     /translation="MDIVRLNSITEFVIGGMNITETVKNSSLAVNLMLLLLVIMSVIS
                     WGLIAYKFIMYRKAVLETEKFISIFWKNMNSEEIFAKSNKMKFSPVAQVFCAGYQEFK
                     DTKESWENKGIAVIGTKVEELAANVERAMKRENQSRTQILESRLTFLATTATASPFIG
                     LFGTVYGIMNAFQEIGKTGNATLGSIAPHLSDALVTTAFGLIAAIPATVFYNYFNSQL
                     QRFENEALNVAIDFINQIKRSNV"
     gene            3760..4197
                     /locus_tag="PL056_07130"
     CDS             3760..4197
                     /locus_tag="PL056_07130"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HPM46825.1"
                     /translation="MQSGFETGRKRSIKSDLNITPMVDIVFVLLIIFMVAAPLIEQGV
                     DLDLPQTETVPIESPKDKFIVRVFKGRKEAQNQIFIDKTEVPLKELEIKLKTNAKLQQ
                     QKEIFLHASRDLSYGYVIRIMAIMKKAGVSNISLVTEPLENEG"
     gene            4200..4973
                     /locus_tag="PL056_07135"
     CDS             4200..4973
                     /locus_tag="PL056_07135"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HPM46826.1"
                     /translation="MTSETRSGSYIFTASNETGERKAVSRFYIYSFSMHIFIIIAFAS
                     GIWMDRNRKIEFDTVQARLVKLGEERDKKLLPRITKQEPVKSSKKEAKANSLKPVEKK
                     DNEKKKEEEKKVVKPPSLAELLSGTMQEIKKDARAEVTKEGATDGDDDGDVTDPALAL
                     KANMYTRKISALIRKNWNIPGIISGNQLKDLQTSAFFRITFSGEVYAIEIISSSGNKI
                     FDSSVLEAIKKTGSLPLPEDRELKKLVLKEGFECPFTPG"
     gene            4952..6256
                     /gene="tolB"
                     /locus_tag="PL056_07140"
     CDS             4952..6256
                     /gene="tolB"
                     /locus_tag="PL056_07140"
                     /inference="COORDINATES: protein motif:HMM:TIGR02800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HPM46827.1"
                     /translation="MSIHSRLINLFFILFISTGLYGQIKVEVRDGAISEYNMAIAPVI
                     NSEGASPAQSKEISDAIGLVLDITGYFKMLDPKSFLEKADVPPSSVDFKNWMNVGANG
                     LIKGTIKGSDNVELELYFFDVAEGKEILRKKYKSSAKAIKKAAHRFASDLITLLTGEE
                     MSFMFSRVCFVEKSGEAYSLISTDFDGSDKKVIYSVKKIILLPEWSADGKKIFFTSYE
                     KSNPNLYSIDVATKQIKLISSHEGLNTSASASPDNKTIALRLSKDGNAEIYLLNTTTS
                     ELSRMTKNMAIDTAPSFSPDGKEIAFVSNRAGNPHIYRLWVNNPERVERLTTQGKYNQ
                     DPDYSPDGKYISFTGRDEFFAFDIFLFDISTRSIARVTQKQGRNESPTFSPDSRLIAF
                     SSDRSGKNGLYLSNLKGDKQILVYAGEGEVVSPSWSPEVIKQ"
     gene            6278..6976
                     /locus_tag="PL056_07145"
     CDS             6278..6976
                     /locus_tag="PL056_07145"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPM46828.1"
                     /translation="MKRFLVLLMISVLAGLTFSGCAKPPKAERELAEKAFKDALVGKD
                     CDKENYLAAEELLNKAREEVNKKNYAKAKELFQAVKEKSDAIVKYYQDNPDKCLPKKK
                     EVKKAQEEIEEIEDPATDPELEFPVIYFEFNEYTIRSEDLTKVDLISRWMNNFPEKVL
                     RIEGHADERGSIDYNMSLGEKRADEVKNRLIQSGIDAKRVKIVSYGEERPADPASNES
                     AWFKNRRAEFKRVN"
     gene            7116..7682
                     /locus_tag="PL056_07150"
     CDS             7116..7682
                     /locus_tag="PL056_07150"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HPM46829.1"
                     /translation="MDKRIFRKDILALRLKMPGDDVLNSSKAIFNTLISLDVFKNSFV
                     VALYAPFKNEADLMPLLDIKGKQFVFPKVKKGTRKLEFYEVTSLKDLTAGSYGILEPV
                     EELVSVNIDKIEFFAVPGVAFSQKCERIGYGGGYYDSTLLHRSLNSTVAGIAYDLQIV
                     ESGFSTDGDQPVDMVITETRIFKNITTS"
     gene            7691..9244
                     /gene="rny"
                     /locus_tag="PL056_07155"
     CDS             7691..9244
                     /gene="rny"
                     /locus_tag="PL056_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006000390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HPM46830.1"
                     /translation="MKMDITSIIMLIIGIPVGALLWSMYKTIAYKEKMERARKDLENA
                     KTQAEKILKESQLKSQENILEGKKATEEQKNELLKEFKEREKRLNKREEQLALRDELY
                     LAKEKEFKVIEKRQKDTETLLETQKESVQNEMKKVTTELEKIAGMSQEEAKEKLIQKI
                     EDEAKHIATKKIKAIEDALAEDAEKMSKEIISTAIQRYSGEYVAENTVTTVSLPNDEM
                     KGRIIGREGRNIRAIEAVTGVDLIIDDTPEAVVISDFNPIRRQIASLTLQKLISDGRI
                     HPGRIEEVFEAASKDVWKTIKEAGEQAIFDLGIHKLHPELVLLVGRLRYRTSYSQNVW
                     KHSIETGFLAGLMAAELNYSVKNARRAGLLHDIGKAVDHEIEGSHASIGAKLAEKYGE
                     SKRIFNAIAAHHGEIKPESILDVLIAAADALSGARPGARREMSEAYIQRLTELENLAT
                     AFEGVDKSFAIQAGRELRVIVDTTKVNDEDAYILANDISKKIEAELSYPGQIKVVVIR
                     ETRVVSTAQ"
     gene            9254..10042
                     /locus_tag="PL056_07160"
     CDS             9254..10042
                     /locus_tag="PL056_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HPM46831.1"
                     /translation="MTFRILMLGDIIGRPGRRAVREKLPDIIKKNEVDFVVANGENAS
                     GGKGLVESAAKDLFSSGIDVLTSGNHIWNKKEIFSFIDKYPQILRPANFPDNISGHGS
                     YIFEQKKKNIKVGIINLIGRVFMSPVDSPFTKAVTEIEKLKSSGADIIIMDFHAEATA
                     EKEALGFYLADKIELFAGTHTHVQTSDEKILSGRCGYITDLGRCGSFNSVLGFEKEDS
                     IKGYLTNIPQSFTPAKEDLVIEGIIAEFDEVSRKCTEIKRIREF"
BASE COUNT         3323 a         2021 c         2263 g         2650 t
ORIGIN      
        1 cagtattcta tataaactga ctctgtttct gaatcacagc tttgataatc acagtatacc
       61 gttttatagt cctgatacac ttcatcatct atgcagtatt tgcttccgac atatccgctt
      121 gtgccgcaat tctccttgat ctcctccatt tcaccgcatg tatcatacca gtagacttga
      181 ccgttatagc atgaatatga accgtgagga tagcagtcgt tactgcataa gccgtcaccg
      241 taacaagtac cgctcttgct gcatgttgcc ggttcacccc acacacctcc ggcacagatc
      301 tgatactgat atgcaccata gcaccgcctg tcaccgttca tacagttctc cggctctatc
      361 tcgacacatg tcgctgtgtc ccatccgctg cagttgtctt tgcattttgc cttgccggaa
      421 gaatattttg tgtcatcaat atcaacacag tccatcgttc cgccgtcaca cacttcaggc
      481 cattctatcg aagtgttccc gcaagtgtca cttattacag aatcttcatc cggtactgag
      541 atgtcatctt ttacagcttc atcatccttg actgtctggt tgtcatcggc aacattttca
      601 tcatctgaaa ccgtctggtc tgaatcattg tctgaaacag aagcatcgtt atcgttttta
      661 ggcttgcttt ttgtagctac agaatctccg cacgaaaaag caaagaccgc aagcatcact
      721 gaaacaacaa aatgtctcat tcccctctcc ctttaaatga aaaaaacaga cgaaatttta
      781 cctgaattat cctttaaatc aattttcctc ttcaaattga cttatttgag ccgttaaata
      841 tattacagta gtttctttgt catattaatc ttatccggag ggtgaaatgt ccgagcttaa
      901 ttacaaaaat gctggagttg acatagtaga aggggcaaaa atggttgaaa agatcaagcc
      961 cctcgttaaa agaacaatga ggcctgaagt tcttgcgggc atcggcggct tttcatcact
     1021 cgtatcgatt ccggaaggaa taaaaaaccc tgttcttgtg agcggaactg acggcgtagg
     1081 aacaaaactc aagttcgcat ttctctcagg gattcacaat actatcggaa tagatctggt
     1141 cgcaatgtgt gtcaacgatg tgatcgtggg cggagccgaa cctctcttct ttcttgatta
     1201 ttttgccaca ggaaaactaa gtgccgaagt cggcgcggaa gttgttgcag gaatcgccga
     1261 aggttgtttt caggcgggat gtgctcttgt cggcggggaa actgccgaaa tgccggattt
     1321 ctacgaatca ggtgaatatg atcttgccgg cttctgtgta ggtgttgtgg atcgcgaaaa
     1381 aattattgac ggatcaaaga tcaaagaagg tgactgcatc atcggaatcc cgtcaacagg
     1441 tgtccattca aacggatttt cgcttgtcag aaagatcgtt ttcaacaaaa tgggtctttc
     1501 gaccggggac atgatagacg aactcggtct tacggttgca caggaatttc tcaaacccac
     1561 aagaatatat gtcaaggaag tgcttgaatt gattaagaaa tttaaaatct ccggtatggt
     1621 tcacatcacc ggcggcggat tttatgaaaa tattccgagg gtcattcctg aaggattggg
     1681 tgctctgatc gacggcaaat ttcctgttct tccggttttc aaatggatga agaactgtgc
     1741 aggcctgaca caaagagaga tgttcacaac attcaactgc ggaaccgggt tcatgcttat
     1801 cgttccggaa gatcaggttg aagaactgct gaaagattcg gacagatact atgtcggaag
     1861 gatcgtaaga accgatgaag tcgaaagagt tgtcataccg ggaaatcatt tttaggggga
     1921 aaaccttgag aaagatcttt tttcttttaa ctttgcctgc ctttattttg ctgtccgccg
     1981 gatgctcatc agcagaaagg acacaaaaca gaaaagatca ggaaaacctt aaactgcttg
     2041 aacagaatgt tgaaagtgcg gacagttttt tcattgaggg aaaagattcg ttcatcaaaa
     2101 acgaatacgg cagtgccctt gaatcattcc agaaaagcag ttacagccag gcacttttct
     2161 atattggatt gacactgagc gaacttggaa aaaaggaaga tgcaaaagaa gttttcatgg
     2221 actgcgcggc aaaaaacatt cttcctgcag aatcatacta caacctttca atgatagctt
     2281 acgaccttgg tgacatcaat ctgtcaaaag aattcgccat aaaatctatc gggctgaagc
     2341 cggaccacac aggcgcactc ttttttgccg gcaacctttt ctatgttgaa agcaatatgg
     2401 ctgaagcgtt gaattattat aacaaagcga tcgctaccga cccttcatct gtcgatctgt
     2461 gggaagctgt tttttctgta tatcttcaga ccgaagagtt tgaaaagggc tgggcgatca
     2521 gggacaaaat tgacagaaac aacaccggga cagtgctcaa tgtcttaaaa gtagctgaaa
     2581 taacagggaa ttttaaggaa ggggcagatt tcccgaaaaa agaactgctt gaagacagtg
     2641 ctgtcaatca gcaggtgcgg atactactta caagagccgg cgaaataaaa aaagcggcgg
     2701 aaatggctca aaaagagatg gaaaaaaata aaaaacctta tcttatcatt gacagaatga
     2761 ctggcgacaa tggatcatat gttatcgtgc tcaaagagag ttccgttttt cttgtatgtt
     2821 ccgccaaaag tgaaattttc attccagttg agatctcttc cggcatcgta aagatcgatg
     2881 gccgcaacag tgtcaaatct catgatgttt caaaagaagc tgtatcggta tgttcagagc
     2941 agaaataaaa aaatgtttca gaatcctgta ttaaagggaa tatttttgta taataaattg
     3001 tttattactt aaccgtgtgt tccggaggag ataaatggat attgttcgac tgaactcaat
     3061 tacagaattt gtgatcggag gaatgaatat tactgaaacg gtaaaaaact cttcgcttgc
     3121 agtaaatctc atgcttttac tccttgttat catgagtgta atttcatggg gacttattgc
     3181 gtataaattc ataatgtata gaaaagctgt tcttgagacc gaaaaattta tctcgatctt
     3241 ctggaaaaat atgaattcgg aagagatatt cgcaaaatcg aataaaatga aattctctcc
     3301 ggtagcacag gtgttctgtg ccggatatca ggaattcaag gatacaaaag agagctggga
     3361 aaacaagggt atcgccgtca taggcacaaa agttgaagag cttgcggcaa atgttgaaag
     3421 ggcaatgaaa agagaaaacc agtcaagaac tcagatactt gaaagccgtc tcacatttct
     3481 tgcaacaaca gcaaccgcat cacctttcat cggccttttc ggaacagttt acggaataat
     3541 gaacgctttt caggagatcg ggaagaccgg caacgcaact ctcggatcta tcgcgcctca
     3601 cctttccgat gcacttgtaa caactgcttt cggtcttatt gcggcaatac cggcaacggt
     3661 tttttataat tatttcaaca gtcagcttca gcgttttgaa aacgaagctc tgaatgtggc
     3721 gatagatttt ataaatcaga tcaagcggag caatgtctga tgcagagcgg atttgaaacc
     3781 ggaaggaaaa gatcaataaa atcagatctt aacattactc ccatggttga tatcgttttt
     3841 gtgcttctga taattttcat ggttgcggct cctttgatcg agcagggtgt cgacctcgat
     3901 ctgccacaga cggaaacagt tccgatcgag tcacccaaag acaagttcat tgtgcgggtt
     3961 ttcaaaggaa ggaaggaggc acagaaccag atcttcattg acaaaacgga agttcctttg
     4021 aaagaacttg aaataaaact taagaccaac gcgaaacttc aacagcagaa agagatattt
     4081 ctccatgcga gcagagatct ttcgtatggt tatgtcatca gaataatggc aataatgaaa
     4141 aaagcggggg tcagcaacat atccctcgta actgaaccac ttgaaaatga aggataaaaa
     4201 tgacatctga aaccaggagc ggatcataca tatttaccgc atcgaacgaa accggtgaaa
     4261 gaaaagctgt gtcccgtttc tacatctatt cattttcaat gcacattttc ataatcatcg
     4321 cctttgcatc cgggatctgg atggatagaa acagaaaaat tgagtttgac acagttcagg
     4381 cacggcttgt aaaactgggc gaggaaagag ataaaaaact cctgccgaga ataacaaaac
     4441 aggagcccgt taaatcttca aaaaaggaag caaaggcaaa ctcccttaaa ccggttgaga
     4501 agaaagataa tgaaaaaaag aaggaagaag agaaaaaagt tgtcaaaccg ccttctcttg
     4561 cagaacttct ttcaggaacc atgcaggaga taaaaaaaga tgcccgtgcc gaagtgacaa
     4621 aagaaggggc gactgacgga gatgatgacg gcgatgtcac cgaccctgca cttgcgctca
     4681 aggcgaatat gtataccaga aaaattagtg cgctcataag aaagaactgg aacatccccg
     4741 gaataatatc cggaaaccag cttaaagatc tgcaaaccag cgcttttttc agaataacct
     4801 tctcgggaga ggtttatgcc atagagataa tttcttcatc cggaaacaag atatttgaca
     4861 gctcagtgct tgaagctatc aaaaaaaccg gatcgctgcc tttaccggaa gatcgtgaat
     4921 taaaaaaact tgtactgaag gagggatttg aatgtccatt cactccaggc tgatcaattt
     4981 gtttttcatc ctttttattt caaccggact ttacggtcag ataaaggttg aagtaaggga
     5041 cggagctata tcggaataca atatggcgat tgctccggtt attaactctg aaggtgcttc
     5101 gcctgcacag tcaaaagaga tttcggatgc tatcggcctt gtgctcgata tcactggata
     5161 tttcaagatg cttgacccta aatcatttct tgaaaaagcc gatgtgccgc cgtcatcggt
     5221 cgattttaag aactggatga atgtgggtgc caacggcttg atcaagggaa cgatcaaagg
     5281 aagtgacaat gttgaacttg agctgtattt ctttgatgta gcggaaggaa aggaaattct
     5341 gcgcaaaaaa tacaaatcat cggcaaaagc cataaaaaag gccgcacacc gttttgcaag
     5401 cgatctcatc acgcttctga cgggagaaga aatgagcttc atgttcagca gagtgtgttt
     5461 tgtcgaaaaa tcgggtgaag catattccct cataagcaca gattttgacg gatctgataa
     5521 aaaagtgatc tattccgtta aaaagataat ccttcttccg gaatggtccg ccgatggaaa
     5581 aaagatattc tttacaagct atgaaaaaag caacccgaac ctttattcaa ttgatgttgc
     5641 gacaaaacag ataaagctta tttcaagcca cgaggggctt aatacatctg cttctgcaag
     5701 tcctgataac aaaactatcg ccctgcggct ttcaaaagac ggaaatgccg aaatatatct
     5761 gcttaacaca acgacaagcg aactttcaag gatgacaaag aacatggcca tcgacactgc
     5821 accgagcttc agtccggacg ggaaagagat agcttttgtt tcaaacaggg ccggcaatcc
     5881 gcacatatac cggctctggg taaacaatcc tgaaagggtg gagcggctta caacacaggg
     5941 aaaatacaat caggacccgg actacagccc ggacggcaaa tacatttctt tcacagggcg
     6001 ggatgaattt tttgcttttg atatattcct ctttgacata tcgacaagat cgatagcgag
     6061 ggttactcag aaacagggga gaaatgaatc accgacattt tcacccgaca gccggctcat
     6121 tgcattttct tcagacagat cgggtaaaaa cggactttat ctttcaaatt tgaaaggtga
     6181 caaacagatt ttggtttatg caggcgaagg agaggttgtt tccccttcat ggtcgcctga
     6241 ggttataaaa cagtaatttt atatataggg gtgtgttatg aaaaggtttt tggtgctttt
     6301 aatgatttcg gttcttgctg gtttaacttt ctcagggtgt gcaaaaccac ccaaagcaga
     6361 aagggaactt gcggaaaaag cttttaaaga tgctttagtg ggaaaggatt gcgacaagga
     6421 aaattatctt gccgcagaag aacttctcaa caaggcaaga gaggaagtca acaaaaagaa
     6481 ttatgctaaa gccaaggaac tttttcaggc agtaaaggaa aaaagcgatg ctattgtaaa
     6541 atactatcag gacaatcccg ataaatgtct gcctaagaaa aaagaggtga aaaaagctca
     6601 agaggagatt gaagaaatcg aagaccccgc aacagacccc gagctggaat ttccggtcat
     6661 ctattttgaa ttcaacgaat atacgataag atctgaagac ttgacgaagg tcgacctcat
     6721 atcaagatgg atgaacaact ttcctgaaaa agttctgaga attgagggac atgcagacga
     6781 aagaggaagc attgactaca acatgtccct tggagaaaaa agagctgatg aggtcaaaaa
     6841 ccgacttatc cagtcaggaa tagatgcaaa aagagtaaag atcgtttcat acggtgaaga
     6901 aagacctgca gaccctgcat caaatgaatc agcatggttc aagaacagaa gagccgaatt
     6961 taaaagggtg aattgatcat tttccgattt tcttcttgac attttcaaca aattcagcta
     7021 ttttcattgc aacttgcatc cgctgttttt ataaaccgga tgttttagat atatataaat
     7081 ataaccggag gaagccctcg gggctttttg atactttgga taaaaggatt ttccggaagg
     7141 atatcctggc tctcagattg aagatgccgg gtgatgatgt tttaaattca agcaaagcaa
     7201 ttttcaacac tttgatttca cttgatgttt tcaaaaactc ttttgtcgtt gccctgtatg
     7261 cgccttttaa aaacgaagcc gaccttatgc cgcttcttga cattaaaggc aaacagtttg
     7321 ttttccctaa agtgaaaaaa ggaaccagaa agcttgagtt ttacgaagtg acatctttga
     7381 aagatctcac tgccggcagt tatggaattc ttgaaccggt agaagaactt gtcagcgtta
     7441 atatcgataa aatagaattt tttgccgttc ccggagtcgc cttttcgcaa aaatgcgaaa
     7501 ggatcggtta cggtggcgga tactacgatt caactctttt gcacagatca ttaaactcga
     7561 cagttgccgg tatcgcctat gatctccaga tagttgaatc aggtttttct accgatggtg
     7621 accagccggt agatatggta attacagaaa cccgtatttt taagaatatt acaaccagct
     7681 aataaaagga gtgaaaatgg acatcacatc aataatcatg ctgatcatcg gtattccggt
     7741 aggcgcactg ctgtggtcaa tgtacaagac cattgcatac aaggaaaaga tggagcgggc
     7801 aagaaaagac cttgaaaatg ctaaaactca ggcggaaaag attctcaaag aatcacagct
     7861 caagtcccag gaaaacattc ttgaaggcaa aaaggcgacc gaagaacaga aaaatgaact
     7921 tttaaaagaa ttcaaggaaa gggaaaaacg gcttaacaaa cgggaagaac agctcgcgtt
     7981 aagagatgaa ctctatctgg caaaagaaaa agagttcaaa gttattgaaa aaagacagaa
     8041 agacaccgaa accttacttg aaacacagaa ggaatctgtt caaaatgaaa tgaaaaaggt
     8101 tacgaccgag cttgaaaaaa tagccggcat gtctcaggaa gaggcaaaag aaaaactgat
     8161 ccagaaaatt gaagatgaag caaagcatat cgccacaaaa aagataaaag cgatagaaga
     8221 tgcacttgca gaagatgctg aaaaaatgag caaagagatc atttcaactg cgatccagag
     8281 atattcaggc gaatatgttg ctgaaaatac tgttactact gtcagtctcc cgaatgatga
     8341 aatgaaagga agaataattg gtcgtgaagg aagaaatatc agggcaatag aagctgtcac
     8401 aggtgtcgat ctgataattg acgacacccc tgaagctgtt gttatcagcg atttcaatcc
     8461 aatcagaaga cagatcgcat cacttactct gcagaagctc atttcagatg gaagaattca
     8521 tccgggcaga atagaagaag tttttgaagc ggcatcaaag gatgtctgga aaaccatcaa
     8581 ggaagccggt gagcaggcga tcttcgacct tggaattcat aagcttcatc ctgaacttgt
     8641 ccttcttgtc ggaagattga gatatagaac aagctacagt cagaatgtgt ggaaacattc
     8701 aattgaaacc ggatttcttg ctgggcttat ggcggctgag cttaattaca gcgtcaaaaa
     8761 tgcaagaagg gccggacttc tccatgatat aggaaaagcg gttgaccacg agatcgaagg
     8821 aagtcacgcg tcaataggtg caaagcttgc cgaaaaatat ggtgaatcaa aacggatatt
     8881 taacgctatt gcggctcatc acggcgagat caaaccagaa tcgatccttg atgttctaat
     8941 tgcagcggct gatgcactca gcggcgcaag accgggagca agaagagaaa tgtctgaagc
     9001 ttacattcag cgtttaaccg aacttgaaaa tctggcaaca gctttcgaag gtgtcgataa
     9061 atcttttgcc attcaggcag gaagagaact tcgcgtaatc gttgatacca ccaaagttaa
     9121 tgatgaagat gcctacatac ttgccaacga tatttccaag aagatcgaag ctgaactttc
     9181 atacccggga cagatcaaag ttgttgtaat tagagaaaca agggttgtaa gtaccgctca
     9241 atagaggatt ttaatgacct tcagaatttt aatgctcgga gatataatcg gtcgacccgg
     9301 cagaagggct gtccgcgaaa aacttcctga tattattaag aaaaacgaag ttgactttgt
     9361 cgttgccaat ggtgagaacg catcaggagg caagggtctt gttgaatctg ccgccaaaga
     9421 tcttttcagt tccggaatag atgttcttac ctcgggaaac catatctgga acaaaaagga
     9481 gatcttttcg ttcattgata agtatccgca gatattacga cctgcaaact ttcccgacaa
     9541 tatctcaggt catggttcat acattttcga gcaaaaaaaa aagaatataa aggtggggat
     9601 aatcaatctc atcggcagag ttttcatgtc tccggttgac agccctttta caaaagctgt
     9661 tacagaaatt gaaaaactga aaagttccgg agcggatatc ataataatgg attttcatgc
     9721 tgaagcaacg gctgaaaaag aagcgcttgg tttttatctt gctgataaaa ttgaactttt
     9781 tgccggaacc cacacccatg tacagacatc ggatgaaaaa atactttcag gaagatgcgg
     9841 atacatcaca gatcttggca gatgcggatc gttcaattca gtcctcggat ttgaaaaaga
     9901 ggacagcata aaaggatatc tcaccaatat tcctcaaagt ttcactccgg ccaaagaaga
     9961 tcttgttatt gaaggaataa ttgctgaatt tgatgaagtt tcaagaaaat gtactgaaat
    10021 aaagaggatc agggaattct gatcgtccgg cagttcaatt catgctcaag gagggttcga
    10081 tggaaacaaa aaagaaaact ttgacaagag ctgaagcgac aggttcttcg ttgctccggc
    10141 tccagacaaa caccaatttc ggcaagaagg atgagccctt tttccttgtc agcacacttc
    10201 cgggtgatat ttttgtcctt cagagacttg agttctcagg gactgaattt gtttata
//
LOCUS       DAOOYB010000097        10217 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_7098_length_10217_cov_8.328380, whole genome shotgun sequence.
ACCESSION   DAOOYB010000097 DAOOYB010000000
VERSION     DAOOYB010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10217
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7098_length_10217_cov_8.328380"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..1495)
                     /locus_tag="PL056_07165"
     CDS             complement(53..1495)
                     /locus_tag="PL056_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011307971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HPM46832.1"
                     /translation="MNSRVKKILDLFEEINKVPRKSKNEAKISQWLMDWAEKHGFEAE
                     RDEVMNVLIKVPATAGYENKPVIVFQGHMDMVCEKTPESRHDFSKDPIKHVYNGDWLK
                     ADCTTLGADNGIALAIALELATDKTVLHPPMELLFTVDEETGLTGANALKPGWLKGKV
                     LLNLDSEDEGIFTVGCAGGRDTKINFKPEWVKPCSECKNVEIKVAGLSGGHSGVDIKL
                     QRGNANQILARILDNLAASCELKIVSIDGGSAHNAIPRDAFSIISVKKDDLAKVSDIV
                     EKTAAKIKAEFKKTDPDMTITVKEMKDLSDPVMSSSDSVKLINMMMSMPHGVAAMSGD
                     IEGLVETSSNFATVKMSEGVVSLLSSQRSSVASRIEWITRKVEKSAFVADAEFKSGNG
                     YPGWEPDMESGLLAKCISIYEKLYGTKPVVEVIHAGLECGIIGSKNEGMEMISYGPTI
                     KNPHSPDEKMFVPSIEKIWDFTVELLKSYC"
     gene            complement(1598..2824)
                     /locus_tag="PL056_07170"
     CDS             complement(1598..2824)
                     /locus_tag="PL056_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019908003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPM46833.1"
                     /translation="MSGVKLNNALIKHVDELKAKGIAKGAEKVITKVIPAKGDKGPRF
                     IVEGYGDREFIKMNSNSYLGLSLRKDVMAAEEHGTATYGAGPGAVRFISGTYRPHIEL
                     EERLAKFHGRESGMIFSSAYVTSLGVITPLTTAETVIISDELNHNCIINAMKLARPSA
                     KLIYKHNDMADLEAKLNESVGKGKRCIVITDGVFSMRGDFCPFDQFVAICKKFDDKFE
                     EGVTTVADDSHGVGAYGITGRGTEEVTGAKVDILIGTLGKAYGINGGYVVADKAVTDF
                     LRQTAPMYIYSNPITVGETTAVLKVLDILESPEGIKILKHLSEMTARFEKGLVDNGYE
                     TIPGPHPVTPLMVRDTQKTSQIVQWLTDKGVLATGLNYPVVPKGDEEIRFQINGDHTP
                     ADIDYVINVLKEYKNK"
     gene            complement(2840..3790)
                     /locus_tag="PL056_07175"
     CDS             complement(2840..3790)
                     /locus_tag="PL056_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPM46834.1"
                     /translation="MKKILIIGAVGQIGSELTLKMREIYGNENVVASTRKTEPSQKIK
                     ESGPFEFFDVMDRQKLGEVCDKYKIDHMVNMAAILSAVGEKNPLMAWDVNMNGLINVL
                     EVAREKNMKQVLMPSSIAVFGPETPKVDTPQETVLKPTTMYGVTKVAGELLCDYYVRK
                     FKLDVRGLRYPGIISHETLPGGGTTDYAVAIYYDAIQHGKYTCFVKEDTKLPMMYMPD
                     CLKATIDLFEADFGKLKHHSDFNVAGMSFTVSEMADEIRKHIPSFEIRYEPDFRQAIA
                     DSWPDSIDDSAARAEWGWKPAYDLASMTKDMLEQLEKKLK"
     gene            complement(3822..4469)
                     /locus_tag="PL056_07180"
     CDS             complement(3822..4469)
                     /locus_tag="PL056_07180"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HPM46835.1"
                     /translation="MDIFVIFEKGGVIMYPILLCSVISITILFERMFSLRRSKIASNP
                     ELIIHLSEKGSWDEIATEVKKKDDLFSKILDLVIADNTGQDPVKVTEMYAKKVSADIY
                     RWISIPGIMATISPLLGLLGTVLGMIKIFTRFTEAGGNPMILAGGIWEALITTAAGLT
                     VAIPSLVIYRYLNYRADESVSELEFVLEKVLLFRSGNMLSEKEKGNGSVILKGDK"
     gene            complement(4561..>4953)
                     /locus_tag="PL056_07185"
     CDS             complement(4561..>4953)
                     /locus_tag="PL056_07185"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPM46836.1"
                     /translation="KPEVKPEVKPLQPPVTQKPAQKTGEKLSSVVSFMHNSDYIQDAL
                     EIEKVAMILNRNFTLKIRIEAHRDPSEKSTYPQKRADAVKAVLVKNGVEPSRIAIKLM
                     GASEPVAAGDTEPDMAKNRRVEFYMVTE"
     assembly_gap    4954..5053
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5054..6026)
                     /locus_tag="PL056_07190"
     CDS             complement(<5054..6026)
                     /locus_tag="PL056_07190"
                     /inference="COORDINATES: protein motif:HMM:NF024946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HPM46837.1"
                     /translation="MKPAVNKLIILMFLIIMSDLLYAGGFSIDANRFKSTPFEDGYFS
                     VYGAEGLDQDLFGAGFLMHYMPDSISQFNAEGDIIRSVIENQITADISFFYSPVKYFD
                     FGLVFPVMIYQNGDGWDEEDSLPSGGIGDIRLVPRFQIANVSDGLFALSLIPEMTIPT
                     GELVHSALGSSLFTFKPAIAIGSESKRVGFTTNLFYQILQKQYYAESVFDDEFGVKVA
                     LNLHAVPQLFDIVAEFHANTSIAEPFKNEAVDNIELGGGFKVKTPVDIDVSAGAYGGF
                     GSAVGVPKFRVFLGISWSMDVSKKQVETPKPEVKPEVKPEVKPEVKPE"
     gene            6195..7838
                     /locus_tag="PL056_07195"
     CDS             6195..7838
                     /locus_tag="PL056_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HPM46838.1"
                     /translation="MDFQKHINELLSTHPIVQMNMSRELLIYNAVSKGKALITENGAL
                     ATWTPVESTGRSPKDTLIVKHDTSAHKIDWHSPNNRPLDPEVFDMIFEDAIKTLRGKK
                     SFYVNDRVLGADPSYALPVRVITDKAFTSVFVDNMFRPVPKEIEKSIFFKKPFTLIAL
                     PYDKLDQQKYKGKLRRLPDGKTTNMVIAMDFDRSIGIVYGSAYLGSVKKLMFTVMNFY
                     LPDNNILPLHCSANEGPDGNTALLLGLSGTGKTTLSADPERALIGDDEHGWNDNGIAN
                     FENGVYAKLINLKKDKEPEIYNALFHKADYLEHGAMMENAMMYPDGVIDLDDDRLTPN
                     SRASFPLSFLSNIKESACGGHPSTIIFLTADANGVLPPVARLTKNQAMLWFLMGYTSK
                     LAGTETGITEPVSTFSRFFGEPFMPRNPNDYIKLLGEKMEKHNVGVYLVNTGWSGGPY
                     GTGSRMDINITRKIVSACLNGSLENVKYIEDKQFHLEIPETCPGVDPKMLVPINTWEN
                     QSAFKYRAEKLAEEFSHHFDAAYGLRNIPEEVRSQCPGK"
     gene            complement(7842..9500)
                     /locus_tag="PL056_07200"
     CDS             complement(7842..9500)
                     /locus_tag="PL056_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46839.1"
                     /translation="MKKIFLILSVVALIFISCNSKKKTVPTDGEITDNDSVSADDDDS
                     GTLPDSVENDETGEIDDQTVEPDSEVDQDIVDEDPYPYLECDIETKNCGETEICLFVW
                     EKGKYYCLPSCDPEATEPCEGEKVCEPVFEETNYGCFPRLRVSGKVFNLESEMYEPVE
                     GATVVGMFTSGISTDTILTDSLGNYEFFVRMLRNNMGLPVTKEALTLSVSAMGFEQVP
                     GILRPSLPITFDNVSCSAAFCNVSSGFLSIGLYPLDDSVLRYKINGKISEKKGGVLII
                     AEFDFSPCPLGYTDENGEFSIFNVPAGDYSVKAYVKDKNYSAEVVAVVDKDIDGVELN
                     LLETPLSTLNGSVNIVNAPGGSKTSIVLIPASTFIESFSKGILAPGLRAPDYGIEPNV
                     SGNYTITGIPDGKYYVLAAFENDLLVRDPDPNIAGTQILKVTFPADGPVYDISIGNFK
                     VTEAIPIIFPGAEGPELISEPPLFQWERDASATKYIVNVYTAQGTVQWTKEVPKTDDA
                     VMSVQYDGPALKGFYQWIVVSYKNTAPISMSEDLLGIFYTNMQY"
     gene            complement(9509..10135)
                     /locus_tag="PL056_07205"
     CDS             complement(9509..10135)
                     /locus_tag="PL056_07205"
                     /inference="COORDINATES: protein motif:HMM:NF024747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S cluster-binding domain-containing
                     protein"
                     /protein_id="HPM46840.1"
                     /translation="MKVHSIFDSLQGEGRFQGFPTQFVRLYGCNLNCSYCDAQSAVSG
                     DTFDEMPINEIADRIIKSDTYDLCVTGGEPMCQEHELTDLLKNIKGKRISIETNGSFD
                     VTGIGSMFPEVFFSIDWKTPSSGSTDFNMKNIEFLKKGRGWIKFVVGNDSDFDFIADK
                     LKYLENTEIFISPVFEKGRQLFEKTAKFVILNRNLRMQVQLHKVLGIE"
BASE COUNT         2874 a         2346 c         1856 g         3041 t
ORIGIN      
        1 aagaaagaat aaaaaacata acgataacca ttaaaagttt cataaaaccc tcctaacagt
       61 aactttttaa aagctccact gtaaaatccc atattttctc gatactcgga acgaacatct
      121 tctcatcagg gctgtgcgga tttttaatcg tcggaccgta actgatcatc tccattcctt
      181 cattctttga accgattatt ccacattcaa gaccggcatg aattacttca acaacaggtt
      241 tcgttccgta aagtttttca tatatcgaaa tacattttgc aagaagtccg ctctccatgt
      301 cgggttccca gcccggataa ccgtttccgc tcttaaactc tgcatctgcc acaaaagcgc
      361 tcttttcgac ttttcttgtt atccactcta ttctgcttgc aactgaacta cgctgacttg
      421 aaagaaggga aacaacccct tcgctcatct taacagttgc gaaattgctt gatgtttcaa
      481 caagtccttc aatgtcaccg ctcatcgccg caacaccgtg aggcatgctc atcatcatgt
      541 ttatgagttt aacgctatcg gaactgctca tcacgggatc actgagatct ttcatctctt
      601 ttacagttat tgtcatgtcc ggatctgtct ttttgaattc agctttgatc tttgcagcag
      661 ttttctcaac aatatcagaa actttcgcaa gatcgtcctt tttaacactt ataattgaga
      721 aagcgtctct cggaatggcg ttgtgggcgc ttccgccatc tatcgatacg atcttcagtt
      781 cacatgatgc tgcaagatta tcaagtatcc ttgcaaggat ctggtttgca ttccctcttt
      841 gaagcttgat gtcaactccg ctgtgaccgc ctgaaagacc cgcaaccttt atctctacat
      901 ttttacactc gctgcacggc ttaacccatt ccggcttgaa atttatcttt gtgtcccttc
      961 ctcctgcaca gccgactgtg aaaatacctt catcctcgct gtcaaggttc aaaagcactt
     1021 tccctttgag ccatcctggt ttcaacgcat tggcaccggt aagccctgtt tcctcatcaa
     1081 ccgtaaaaag gagttccatc ggaggatgca ggactgtctt gtctgtagca agttcaaggg
     1141 cgatcgcaag agctattccg ttatctgctc ccagagtcgt acagtcagct ttaagccagt
     1201 ctccgttata aacatgcttt atggggtctt ttgaaaaatc gtgcctgctt tcaggtgtct
     1261 tctcacagac catatccatg tgaccctgaa aaacaatgac cggtttgttc tcatatcctg
     1321 cggttgcagg aacttttata agaacattca tcacttcatc cctttcggct tcaaaaccgt
     1381 gtttttccgc ccaatccata agccactgac ttatttttgc ttcatttttg ctttttcttg
     1441 gcactttgtt gatctcttca aaaaggtcaa ggatcttttt cactcttgaa ttcatggttt
     1501 cctcctcaat tcatcaaaaa ttagaaacaa cttataagag taattaattt gaataaaaaa
     1561 gcaaaaagat aaaagagtgt gaaaattatt tcagaaacta tttgtttttg tattctttaa
     1621 gaacattgat aacatagtcg atatcagccg gagtatggtc accgttgatc tggaaacgga
     1681 tctcttcgtc ccctttcgga acgaccggat agttaagccc tgtcgcaaga actcctttat
     1741 ctgtgagcca ctgtacgatc tgcgaagttt tctgagtgtc tctcaccatg agcggagtta
     1801 caggatgcgg accgggaatt gtttcataac cgttatccac gagccctttc tcaaatcttg
     1861 ctgtcatttc tgaaaggtgt ttaagaattt taatcccttc agggctttca agaatatcaa
     1921 gaactttaag aacggccgtt gtttcaccga ctgttatcgg atttgaataa atgtacatcg
     1981 gagctgtctg acggagaaaa tctgtaaccg ctttatcagc tacgacataa ccgccgttga
     2041 tgccgtatgc cttcccgagc gttcctatca gtatatcaac ttttgcacct gtaacttcct
     2101 ctgttccgcg gccggttatt ccgtatgcac cgacaccgtg tgaatcatca gccactgttg
     2161 tcactccttc ttcaaatttg tcgtcaaatt ttttgcagat agcaacgaac tgatcaaaag
     2221 gacagaaatc gcctctcatt gagaaaacac cgtcagtaat gacaatgcag cgcttccctt
     2281 tgcctacgga ttcattcagt tttgcttcaa ggtctgccat gtcattatgc ttataaatga
     2341 gctttgctga aggtcttgca agtttcattg cgttgataat gcagttatga ttgagttcat
     2401 cactgattat aactgtttcc gctgttgtca gaggagttat gacaccaagt gatgtaacat
     2461 aggcacttga aaaaatcatt cccgattctc taccatggaa ttttgcaagg cgttcctcta
     2521 gttcgatgtg cggtctgtat gttcccgaaa taaagcggac agcaccggga cccgcaccgt
     2581 atgtagcagt tccgtgctct tcagccgcca taacatcctt tctcagcgaa agaccgagat
     2641 atgaatttga gttcatctta ataaactctc tgtcaccata tccttcgaca ataaacctcg
     2701 gacctttatc accttttgcc ggaattactt ttgtgatgac tttttctgct ccttttgcaa
     2761 ttcctttcgc cttaagttca tcaacatgtt taataagagc attgtttaat tttacacctg
     2821 acattttaca ctccctggtt tatttaagtt ttttctcaag ctgttcgagc atatcctttg
     2881 tcatggaagc aaggtcgtac gccggcttcc atccccactc tgctcttgca gcggaatcat
     2941 ctattgaatc cggccatgaa tcagctattg cctgtctgaa atcgggttca tacctgatct
     3001 cgaatgaagg aatatgtttt cttatttcat cagccatttc agaaactgta aagctcattc
     3061 cggcaacatt gaaatcggaa tggtgcttaa gtttgccaaa atccgcttca aaaaggtcta
     3121 tcgtggcttt cagacagtca ggcatataca tcatcggaag ttttgtgtct tcttttacaa
     3181 agcatgtgta ttttccatgc tgaatagcat cataataaat agcaacagca taatctgtcg
     3241 ttccgccacc cggaagcgtt tcatggctga ttatgccggg atatctgagt cctcttacat
     3301 caagtttgaa ctttctcaca tagtagtcac acaagagttc gcccgcaact tttgtaacgc
     3361 cgtacatagt tgtcggctta agaactgttt cctgcggggt atcaactttc ggagtttccg
     3421 gaccgaaaac agcaatggag cttggcataa gaacttgctt catattcttt tcacgcgcta
     3481 cttcaagaac atttatgagt ccgttcatat taacatccca cgccatcaac ggattttttt
     3541 cacccacagc cgaaaggata gcagccatgt tgaccatgtg atcgatttta tatttgtcac
     3601 aaacttcacc aagtttctgt ctatccatga catcgaaaaa ttcaaaagga cctgattctt
     3661 taattttctg tgacggttcg gtctttcttg ttgatgcaac aacattttca ttaccataga
     3721 tctcgcgcat cttcagagta agttcagaac cgatctggcc tacggcacca atgatgagaa
     3781 tttttttcat tttttccacc tttttaaaca atttttgtta attatttatc tcccttgaga
     3841 ataacacttc catttccttt ttctttttct gacaacatat ttcccgacct gaaaagcaga
     3901 actttttcaa gaacgaactc cagctctgaa acactctcgt cagccctgta attaagatac
     3961 ctgtagataa cgagactcgg gattgccacc gtgagacctg ccgcagtagt gatcagcgct
     4021 tcccatatgc ctcctgcgag tatcatgggg ttacctcctg cttcagtaaa tcttgtaaat
     4081 atctttatca tgccgagcac tgttccgaga agaccgagaa gagggcttat agttgccatt
     4141 attccgggaa ttgaaatcca tctgtatatg tcagcgctaa ctttctttgc atacatttct
     4201 gtaactttca caggatcttg tcccgtattg tcagcgatca ccagatcaag tatcttcgag
     4261 aaaagatcgt cctttttctt aacttcagtc gctatttcat cccatgatcc tttttcggaa
     4321 agatggatta tcagctccgg attggatgct atcttagatc ttcgaagaga gaacatcctt
     4381 tcaaaaagta tcgtgatgct tatgacagag cataaaagga tcggatacat tattacccca
     4441 cccttttcaa aaatcacaaa aatgtccatc taaacacctc aaaaaatatc cactgtctta
     4501 tactaaaaat gatttaggtt atcaagtaat ttttaatcag aatatttata tgttccgcag
     4561 ctactctgtc accatgtaga attcgaccct tctgttcttt gccatgtcgg gttcagtgtc
     4621 tcctgcagca acgggttcag atgcacccat gagctttatg gcgattcttg agggttcgac
     4681 accgttcttg acaagcaccg cctttactgc atcagctctt ttctgaggat aagtgctctt
     4741 ttcagaagga tcacggtgtg cttcaatccg tattttcaaa gtaaaattcc tgttaagtat
     4801 catggcaact ttttcaattt caagagcatc ctgtatgtaa tcggaattgt gcatgaagct
     4861 tacaacagat gaaagctttt ctcccgtttt ctgagccggc ttctgggtaa caggcggctg
     4921 caaaggtttc acttccggtt tcacttccgg tttnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5041 nnnnnnnnnn nnncttccgg tttcacttcc ggtttcactt ccggtttcac ttccggcttc
     5101 acttccggct tcggagtttc aacctgtttt tttgaaacat ccatactcca tgaaattcct
     5161 aggaaaactc tgaatttagg aacacccaca gcgctcccga atccgccgta agcacctgca
     5221 gaaacatcaa tatcgaccgg tgttttcact ttgaatccgc caccaagctc tatattgtca
     5281 acagcttcat ttttaaacgg ctctgctatt gaagtgtttg cgtgaaattc tgcaacaata
     5341 tcaaaaagtt gaggaacagc atgcagatta agcgcaactt tgacaccgaa ttcatcatca
     5401 aaaacagatt cggcataata ctgcttctga agtatctgat aaaacaaatt cgtcgtaaat
     5461 ccaacccttt ttgattcact tcctattgca attgccggtt taaaggtgaa aagcgaactt
     5521 ccaagtgcag aatggacaag ttcgcctgtc ggaattgtca tttcggggat cagggaaagg
     5581 gcgaacaacc cgtcagaaac attagctatc tggaatctcg gaacaagtct tatgtcccct
     5641 attccgcccg atggaagaga gtcttcttcg tcccatccgt caccgttctg atagatcata
     5701 acgggaaata ccagtccgaa atcaaaatat ttaaccgggc tgtagaaaaa agatatgtcg
     5761 gcagttatct ggttctcaat gacactcctt attatatcgc cttcagcatt gaactgtgaa
     5821 attgaatcgg gcatatagtg cataaggaaa ccggcaccga aaaggtcctg atcgagaccc
     5881 tctgctccgt aaacagaaaa atatccgtct tcaaacggtg ttgacttaaa cctgttggca
     5941 tcgatagaaa agccaccggc ataaagaagg tcagacataa ttatcagaaa cattaaaata
     6001 atcagcttat taactgcagg tttcattttt actccatgac aaaaaacatt aaaaaagcaa
     6061 tgctattata cctttttacg gcaaaaaaac aattattagc attttttttg atattctgct
     6121 gataatacta tatattcatt tccgcttgag cttattaaca attattacta atctttaaaa
     6181 agaaggagtc ggaaatggat tttcagaaac atatcaatga gttactttca actcatccga
     6241 tagttcagat gaacatgtca agagagcttt taatttacaa tgcagtgtca aaaggaaaag
     6301 ctctgataac cgagaacggt gctctggcaa catggactcc tgttgagtcc acaggtagaa
     6361 gccccaagga cactctcatc gtaaaacacg atacaagtgc tcataaaatt gactggcatt
     6421 ctcctaacaa ccgccctctc gatcctgaag tttttgacat gatatttgaa gacgccatta
     6481 aaacattacg cggcaaaaaa agtttttatg tcaacgacag ggttcttggt gcagatccgt
     6541 catacgctct tcctgtaagg gtaataaccg acaaggcatt cacttccgtt ttcgtggaca
     6601 atatgttcag acctgttccg aaagagatcg aaaagagcat tttctttaaa aaaccgttta
     6661 ctctgatagc tcttccttac gataaacttg atcagcagaa atataagggg aaactgagac
     6721 ggcttccgga cggcaagaca acaaatatgg tcattgcaat ggattttgac cgttcgatcg
     6781 gtattgttta cggttcggca tatcttggaa gcgtaaaaaa gcttatgttc actgtaatga
     6841 acttctatct tccggacaac aatattctcc cccttcactg cagcgcgaat gaaggtcctg
     6901 acggaaacac cgcacttctt ctgggactta gcggaacagg caaaacgact ctttcagcag
     6961 acccggaaag ggcgttgatc ggtgacgatg aacacggatg gaatgacaac ggtatcgcaa
     7021 attttgaaaa tggagtttat gcaaaactta ttaatctgaa aaaagacaag gaaccagaga
     7081 tatacaatgc tcttttccat aaagctgatt atcttgaaca cggtgcaatg atggaaaacg
     7141 ctatgatgta tcctgacggt gtgatagacc ttgatgatga ccgtctgaca cctaattcaa
     7201 gggcaagttt tcctctttct ttcctttcaa atatcaaaga aagtgcctgc ggcggtcacc
     7261 cttcgacaat aatttttctt accgctgatg caaacggtgt tcttcctccg gtagcacgac
     7321 ttacgaaaaa tcaggcaatg ctctggtttt taatgggtta cacatcaaaa ctggctggaa
     7381 ctgaaacggg aataactgag ccggtttcca ctttctcaag attcttcgga gaaccgttca
     7441 tgccgaggaa tccgaatgat tatattaaac tgctcggcga gaaaatggaa aagcataatg
     7501 tcggtgtata tcttgtaaac accggttgga gcggcggacc ttacggcacc ggctcaagaa
     7561 tggatataaa tatcacaaga aagatcgttt cagcttgcct gaacggttct ctcgaaaatg
     7621 ttaaatatat cgaggataaa cagttccatc ttgaaatccc ggaaacatgt cccggtgttg
     7681 acccgaaaat gcttgttccg ataaacacat gggaaaacca gtcagctttc aaataccgtg
     7741 cagaaaaact tgctgaagag ttctcacatc actttgatgc cgcttacgga cttagaaaca
     7801 tccctgaaga agtcagatca cagtgccccg gaaaataata ttcaatactg catattcgta
     7861 taaaaaattc caagaagatc ttcagacatt gagatgggtg cggtgttctt gtaacttaca
     7921 acaatccact gatagaatcc tttaagtgca ggcccgtcgt actgaacgct catgaccgca
     7981 tcatctgttt taggaacttc ctttgtccat tgcactgttc cctgtgcagt ataaacattg
     8041 acaatatatt ttgtcgcact tgcatctcgt tcccactgaa aaagcggtgg ttcgctaatt
     8101 aattcaggac cttccgctcc tgggaaaatt atcgggattg cttcggtgac tttgaaattt
     8161 cctattgaaa tatcataaac cggaccatcc gccggaaatg ttactttgag tatctgcgtc
     8221 ccggcaatgt tcggatcagg atctctcaca agaagatcgt tctcaaaagc tgcaagaaca
     8281 taatactttc catctggaat tcctgttatg gtgtaatttc ctgaaacatt gggttcaatt
     8341 ccgtaatcag gagctctaag tcccggagca agtatccctt ttgagaaaga ttcaatgaaa
     8401 gttgaagcag gaataagaac aatgcttgtc ttcgatccgc caggagcgtt gacaatattt
     8461 accgaaccgt taagcgttga aagcggagtt tcaagaagat ttagttcaac gccgtcaata
     8521 tctttatcaa caacagcaac cacttcagca ctgtaattct tgtccttcac atatgctttt
     8581 actgaataat ctcctgccgg aacattaaat atggagaact cgccgttttc atcagtatag
     8641 ccaagaggac acggggaaaa gtcaaattcg gcaattataa gcactccacc cttcttttca
     8701 gatatttttc cgtttatttt atatcttaac accgaatcat caagcggata aagacctatt
     8761 gaaagaaagc cggagctcac attgcagaaa gctgcagaac agctcacatt atcaaatgtt
     8821 atcggaagcg acggtcttaa tatacccgga acctgctcaa agcccatagc tgaaacagaa
     8881 agggttaacg cctcttttgt cacaggaagt cccatattat tccttaacat ccttacaaaa
     8941 aactcatagt tgccaagaga atcagtgagt atcgtgtctg ttgatattcc ggaagtaaac
     9001 attccgacaa cagttgcccc ttcaaccggc tcgtacattt cagattcaag gttgaagacc
     9061 ttgcccgaaa ctctcaaacg ggggaaacaa ccgtagttag tttcctcaaa aacgggttcg
     9121 caaacctttt caccttcaca aggttccgtc gcttcaggat cacaggacgg aaggcagtaa
     9181 tattttccct tctcccaaac aaacagacag atctctgttt caccgcaatt ctttgtttcg
     9241 atatcacatt caagataagg ataagggtct tcgtcaacga tatcttgatc aacttcactg
     9301 tccggctcaa ccgtctgatc atctatttca ccagtttcat cattttcaac agaatcagga
     9361 agtgtgccgg aatcatcgtc atctgctgaa acagaatcat tatccgtaat ttcaccatcc
     9421 gtcggcactg tctttttctt gctgttgcag gaaataaaga taagtgcaac aacacttaaa
     9481 atcaaaaaga tctttttcat aactcacctc actctattcc caaaacctta tgcaactgca
     9541 cctgcatcct taaatttctg ttaagtataa caaattttgc agttttttca aataactggc
     9601 gacctttctc aaaaacagga cttataaaaa tttccgtatt ttcaagatat ttcagtttgt
     9661 cagcaataaa atcaaagtca gaatcgtttc caaccacaaa ctttatccaa ccccttccct
     9721 ttttaagaaa ttcaatgttt ttcatgttaa aatcagtgct tcccgatgac ggagtcttcc
     9781 agtcaattga aaaaaacact tccgggaaca ttgatcctat ccctgtcaca tcaaatgaac
     9841 cgttggtctc gatcgagatc ctttttccct ttatattttt aagcagatca gtcagttcat
     9901 gttcctggca cattggttcg ccgccagtta cacacaggtc ataagtatct gatttgatta
     9961 ttctgtctgc aatctcattt attggcattt catcaaaggt gtcaccactg actgcacttt
    10021 gagcatcgca gtaactgcaa ttcaggttgc agccgtacag acggacaaac tgagtcggaa
    10081 aaccctgaaa tctcccctca ccctgcagag aatcaaaaat ggaatgtact ttcataacag
    10141 ctcctattta cataaaaaac atcattagaa ggttacggat attcacctct cttgtcaaat
    10201 tcagagatgt tcacttt
//
LOCUS       DAOOYB010000098        10008 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_7315_length_10008_cov_7.227871, whole genome shotgun sequence.
ACCESSION   DAOOYB010000098 DAOOYB010000000
VERSION     DAOOYB010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10008
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7315_length_10008_cov_7.227871"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="PL056_07210"
     CDS             <1..252
                     /locus_tag="PL056_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46841.1"
                     /translation="RTDLKKSFCSLPTPHAEGKMEYRQGAPEYEGHWLVWQDSVGPLM
                     KVRDMECYCDYHPELCPFDDYTPQPDNPKDVKTGKRVKE"
     gene            377..3238
                     /locus_tag="PL056_07215"
     CDS             377..3238
                     /locus_tag="PL056_07215"
                     /inference="COORDINATES: protein motif:HMM:NF015194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005471 - ATP:ADP antiporter activity
                     [Evidence IEA];
                     GO_process: GO:0006862 - nucleotide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Npt1/Npt2 family nucleotide transporter"
                     /protein_id="HPM46842.1"
                     /translation="MNKVFSMLNFKDDERKRAGLLFIYQFLAVAVMVQGRIVRDTLFL
                     KRYDTSKLSLMYIGVAILVSTATYLYIKRSFFYRLDKLIIGTFFIGIFATIIFVFLVK
                     ANVKISFPMLYIFIELLGAFMMFQFWSFTNELIDSREAKRVLGFIGGGGILATLIIGG
                     SIKQFVNFMPVENLLIINAVFMGICMNIVRSMGTRYQMRLQRGIVAKTATKLTGKTKI
                     SIFRSPYVKYIALMTAFIFVVTTLVDYQFKIVAGKNFSEKELATFFGMVYAIFGGVFS
                     LFFQFIATSRLLRWSIFVSLGILPVMMTLFSVFFITLPETLILFSWSAPLVAITMAKA
                     SDYAFRYTINDAAMQLLYIPLDPKIKSRAKALIDGIIKPTFIGISGLILYAVNMTKTN
                     ENSISWFVVVIGIVWIIVIIGIRKEYLSVLIDNIKKKRFGTSDLAVKQNIFENIITKA
                     IESGDEEEILMALDMIEKGNLYGMGRNFIPLLSNSSSRIKVKILALLRSMESRFYTYD
                     ILKLLKDKDDDVVKEAVLTYGYMQMEKSINYLSQFLESDNIVIKRSAITALIKFGGVS
                     GVMVAAPYLKELTDSGDEVKRYSAAYILGEIGQRNMQQQVFNLLNDPSPNVRREAVKA
                     ACKIGSKIFIPKLFYMLFDKNVSFDVSKALSQFGEKVLPPAADILNNSLESYRLKSEV
                     ARMLGDVYSPKAVHLLMNTLDTKSDEMRNIVLNSLKKLVSKVDNISLNIEDLRIMLFK
                     EIFQYFQLLYFAVKIRERLKTGHLSTVLETKLQNCYQRIFSILSLMYGNTLFDNIYFN
                     ITQKYVSNAQRSNALEIVDNMVDKDLRPIIVPLIELKDEEEKLKLGFAFFKIKKIGFT
                     EIIETFLVDDSEWVRAITISVIATENIIEFGEKIEMFLYDPSPIVRESALHTMELMGI
                     KVPDEDIKCLLDDPDPLVRKYTNYIIQPKNRRTTMILEN"
     gene            3249..3695
                     /locus_tag="PL056_07220"
     CDS             3249..3695
                     /locus_tag="PL056_07220"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HPM46843.1"
                     /translation="MLTRVERVLALKNVELFHDIPGEVLADIASLLEEEMFEKGQYIV
                     NEGDLGKELFIIIKGEVEVVSNGNRIDIMRSGAGFGEMALIDSQPRSADVIALEDVLV
                     LKMERDDFHEILKQREEVAIGVIKVLNRRIRNLNQRLVERSGEKIK"
     gene            3712..4143
                     /locus_tag="PL056_07225"
     CDS             3712..4143
                     /locus_tag="PL056_07225"
                     /inference="COORDINATES: protein motif:HMM:NF021435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2141 domain-containing protein"
                     /protein_id="HPM46844.1"
                     /translation="MKRFFTALFVLIICSMLAGQDKKATLTVVLKGLKVQGTVYVSLY
                     DKDNGFPTKSQNAVKNSIKKVAAATENILFKDLPFGEYAVSVWHDENGNGEMEKSFIG
                     IPKEGMGVSNDAKGKMGPPKFKDAKFNIDKEAKTIEINVKY"
     gene            complement(4140..4511)
                     /locus_tag="PL056_07230"
     CDS             complement(4140..4511)
                     /locus_tag="PL056_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HPM46845.1"
                     /translation="MKKIVLFLSVFLLISASNTKKPVFYFFHSESCPHCVHAEPFIKS
                     LEKKYPEIKFEKLEVSRNFDNKELLIKKIQEFKIESPGVPLFIFGKSYVMGYKKETHD
                     EKIIQMIRKELYPKEMELPKD"
     gene            complement(4516..6903)
                     /locus_tag="PL056_07235"
     CDS             complement(4516..6903)
                     /locus_tag="PL056_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46846.1"
                     /translation="MNRLFLIFIVVSALLISVSCGGGDKNDKCPEGYTWNGSECEKDS
                     VINPDKDTIVNYDEDPKNDTDPADDDSENDESGSDDDSTSYTGDCTEVKAGGTLEINV
                     QTKNLTIGEITINGESDDTNLSGELWGENKATLSEFKIADIDGTLSGKKFKIPVGRYD
                     FLFKSASYENRITLLENIDMASGDRTLDFEIPLFHFTGSVLKNGAAFTVEAGMEDATK
                     IKLKSGTYEKIIPYSDFGAFDVVVPKGTYSVYFEGQLASGQGVFKGTILSSTNGIEIE
                     EDVDSGINVETVTFSGNAVNEGYDVSTGQIIIVENPPFDNPSAVIIPDLATKAYSVTM
                     TKGAQFNVLYLPETDSYPVKYIKLELWDDLSAGKTHDIILDFARLHGTVTFLGNASLP
                     SVSECTGADCTRGKLKLAGFDYSSLVIKDFGTTGEDLTYDALVVRRTKNIVPDPDNPP
                     DGTKVVYNPKTYTMNFESHLNNVKGAFEYSPFTLPLKYLNSESVPVSNFTFQNVAEEY
                     VLEKEINFNITPMLVEGTVTLNGSAFTTEKDDLIKIRDLNGIETPVINLSELSDGTFS
                     FLVPTGEYDVIYEGEGVLGSEFKTYIERNLNVTGNMSGQTLGITTSKIVLGLSINGTP
                     FKEWAQNNKTLNDYQIVVNPDKTAANYLIDIVKPTDGDWHGEVISGLTINAYLDLFFT
                     SSEKEKKSFMRIPLLVSHSMSSDKTVDTSFNITEYSTTVKINSSAVTGASDYIAQLQI
                     QGQNRTEVFYPASGTEAAKALFRSGEHKSPRPELILNEGFDTKQTIELNCIYFGE"
     gene            complement(7007..>8329)
                     /locus_tag="PL056_07240"
     CDS             complement(7007..>8329)
                     /locus_tag="PL056_07240"
                     /inference="COORDINATES: protein motif:HMM:NF012632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46847.1"
                     /translation="TSYSYLEDYEYYCAISSGELFCWGNNDYGQLGDGTFESRSEPVK
                     IGSRNDWEKIFGWHTVTCGIAGGELFCWGRNIGLTADSSVESVNIPTKIGTLNNWSAL
                     TCFGLCGVESGNFYCFESKEKNPLTKVGTLNEWTQISTATQKRCGISGGSLYCSDYLV
                     NSGTYSEFMKITDSSGWDYVSTGDYHSCAIRNEDLYCWGKNNLGQVGDSSSEDRESPV
                     QIGSDRKWESVSCGCSHTCGTSGGNLYCWGSNSSGQIGDGTTYYYDMPQRIGNKIEWQ
                     KVSAGETHTCGIESGELYCWGSEIVYEPLKIGDRSDWEQVVSSDNSSCGIAGGEIFCW
                     GQITANLTGKLRNIPTKVWNYSDFKKISTVGDILCGLRNDKHLYCLSYSSFASGYYAN
                     DLYPEKIAEIEWDSISESGLICGIVDKILYCPFTRQYYPVQLQTIINP"
     assembly_gap    8330..8429
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8430..>10008)
                     /locus_tag="PL056_07245"
     CDS             complement(<8430..>10008)
                     /locus_tag="PL056_07245"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HPM46848.1"
                     /translation="MMKYFILLSLFMVISCDPLQKFERPVNDEDSFSDVDEEQFPDLD
                     SDEISDETGDADSDESMPCGSVYFNGVDSYISVEHDDALNLGNTWTIEAWAMQGNAEN
                     QDPLIRKGSSTESPSYWIYGKDSDALSTFSSAPNGGYQFGTTSDEDVWLHSSKEVQSM
                     KWYHVALVKSETELKLFVDGVLQKKESADKSPVKNSESLYFGVRLNSNPSYFSGLIDE
                     IRFSFIPRYSENFTPEKRLYADEQTIAVWHFEESEGTETASEGKNILKGVLNGSVQFV
                     NECAENEKECVNECFFEGETKCGEGILSTCVKNSDGCLIFESEGCLTGICADDFICAV
                     DDCLFAGMTECVSGSARICRELENGILDWSFPVICSDGVCADDASCLSCTNECEFEGQ
                     TVCSDGKISACVNNNGCNKWEPEHKCSLNKCENSINCKSDGWIDVSGATYKCGINGSG
                     ELHCWRDSPYPEKMSDRTDWSKVTVSSNDPSLSYFCAIASGELYCMGYNANGELGDGT
                     TVSKTEIARIGSRSDWS"
BASE COUNT         2837 a         2034 c         2020 g         3017 t
ORIGIN      
        1 aggacagacc tgaaaaagag tttctgctca ctccccacac cccatgcaga aggaaaaatg
       61 gaataccgtc aaggtgcccc ggaatacgaa ggacactggc ttgtctggca ggattcagtc
      121 ggaccattga tgaaagtaag agacatggaa tgttattgcg attatcaccc tgaattatgt
      181 ccgtttgatg attacacacc tcaacccgat aatccaaaag atgtgaagac ggggaaaagg
      241 gtgaaggagt gatttctcac aaatcaacac aaatcacaat tcagaaagaa gttctttata
      301 aatctcatac ctaaaattgt tttttgtacc gcagtaatta taatatctgg tcatttattg
      361 attttaaggt gtgtgaatga ataaagtgtt ttcaatgctc aacttcaagg acgatgaaag
      421 aaagcgggcg gggcttcttt ttatttatca gttccttgcg gttgcggtca tggttcaggg
      481 aaggatcgta agggatactc tgttcctgaa aagatatgac acatccaaac tttcactgat
      541 gtatatcggt gtcgcgatac ttgtttcgac tgcgacatat ctttatatca aaaggagctt
      601 cttctaccgt ctggacaagc tgataatcgg cacttttttc atcgggatat ttgcaacgat
      661 catatttgtt ttccttgtca aggcgaatgt gaagatcagt tttccgatgc tttatatctt
      721 cattgagctt ctcggcgcgt tcatgatgtt ccagttctgg tcattcacaa acgaacttat
      781 cgattccaga gaggcgaaaa gggttctcgg tttcatcgga ggaggaggta tccttgccac
      841 gcttataatc ggcggaagca ttaaacagtt cgtgaatttc atgccggttg agaatcttct
      901 tatcatcaat gccgttttca tgggaatatg catgaatatt gtaaggtcga tgggaacaag
      961 ataccagatg cgccttcaga gaggcatcgt cgcaaaaacg gcaaccaaac ttaccggaaa
     1021 aaccaagata agcatattca gatcgcctta tgtgaagtac atagcgttga tgacggcgtt
     1081 tatttttgtg gtcactacgc ttgttgatta tcagttcaag atcgttgcag gcaaaaattt
     1141 cagcgaaaag gaactggcga catttttcgg tatggtatat gcaattttcg gtggcgtttt
     1201 ctccctgttc ttccagttca ttgcaacatc aaggctttta agatggagca ttttcgtatc
     1261 gctgggcatt cttcctgtca tgatgacact gttctctgtc tttttcatta ctctgcctga
     1321 aacacttatt ctgttctcat ggtctgcgcc acttgtagcc ataacaatgg cgaaagccag
     1381 cgactatgcg ttcagataca ctataaacga tgccgcaatg cagcttttat acatcccgct
     1441 tgatcccaaa ataaaaagtc gtgccaaagc attgatagat ggaataataa aaccgacatt
     1501 cataggtatt tccggcttaa ttctctatgc agtgaacatg acgaaaacca atgaaaattc
     1561 aatatcttgg tttgttgtag tgatcggcat cgtctggata attgtgataa tcggaataag
     1621 gaaggagtat ctgtcagtcc ttatcgacaa cattaaaaag aaaaggttcg gcacaagtga
     1681 ccttgccgtt aaacagaaca tttttgaaaa tatcattacc aaagccatag aaagcggtga
     1741 cgaagaagag atactgatgg cgcttgatat gatagaaaaa ggtaaccttt acggcatggg
     1801 aaggaatttc attcctcttt taagcaattc gagctcaagg atcaaggtca agatacttgc
     1861 acttttaaga agtatggaaa gccggtttta tacatatgac attctcaaac ttcttaaaga
     1921 caaggacgat gatgttgtaa aagaggccgt tcttacatac ggttatatgc agatggaaaa
     1981 gtcgatcaat tacctttccc agttccttga aagcgacaac attgtgataa aaagatctgc
     2041 aataaccgcc cttataaaat tcggcggagt cagcggagtc atggttgccg caccttatct
     2101 gaaagagctt acagactcgg gtgatgaagt gaagcggtac agtgcggctt acattcttgg
     2161 agaaataggt cagaggaaca tgcagcagca ggttttcaac cttctgaacg acccaagccc
     2221 gaatgtaaga agggaagccg taaaggcggc atgcaagatc ggatcaaaga tattcattcc
     2281 caagcttttt tatatgcttt ttgataagaa tgtaagtttt gatgtgtcaa aagccctttc
     2341 tcaattcgga gagaaagtgc ttcctcctgc ggctgatatt ctgaacaaca gtcttgaaag
     2401 ttaccggctg aaaagtgaag ttgcaagaat gctcggagat gtatattcac ctaaagcagt
     2461 tcatcttctc atgaacacac ttgacaccaa gagcgatgaa atgagaaata ttgttctgaa
     2521 cagtctgaaa aaactggtgt caaaagtaga taatatttca ctcaacatcg aagatctcag
     2581 gatcatgctt ttcaaggaga tattccagta ttttcagctg ctttattttg ctgttaagat
     2641 cagggagcgg cttaagacgg gacatctttc aaccgttctt gagaccaagc tccagaactg
     2701 ttatcagaga attttctcaa ttctgtctct gatgtacgga aatacgctgt tcgacaacat
     2761 ttacttcaac atcactcaga aatatgtttc aaatgctcag cgctcgaatg ctcttgagat
     2821 agttgacaat atggttgaca aagacctccg gccgatcatt gttcccctta ttgagcttaa
     2881 agacgaagag gaaaagctca agctcgggtt tgcattcttc aaaatcaaga agatcggttt
     2941 taccgagata atagaaacat ttcttgttga tgattcagag tgggtgaggg cgataaccat
     3001 atccgtgata gcaactgaaa atataattga atttggtgag aagatagaaa tgttcctcta
     3061 tgatccgagc cccattgtca gggaatcggc tcttcacact atggagctca tgggcatcaa
     3121 agttcctgac gaggatataa aatgtcttct tgatgatccc gatccgcttg taaggaagta
     3181 cacgaattat attatccagc cgaaaaacag aagaaccacg atgatattag agaattaagg
     3241 aggcatttat gctgaccaga gtggaaagag tccttgctct taaaaatgtt gagctttttc
     3301 acgacattcc cggagaagtg cttgcggata tagcgtcgct tcttgaagag gagatgtttg
     3361 aaaaagggca gtatattgtg aatgaaggag atctcgggaa ggaacttttc ataattatca
     3421 agggtgaagt ggaagtggtt tcaaacggca acagaatcga cataatgaga tcaggagcag
     3481 gtttcgggga gatggctctt attgacagtc agccgagaag cgcagatgta atagcacttg
     3541 aagatgtcct tgtgcttaaa atggaaagag atgattttca tgagatactc aaacagcgcg
     3601 aagaagttgc catcggggta attaaggtgc tgaacagaag aataaggaac ctcaaccagc
     3661 gtcttgtaga aagatcaggc gaaaagatta aataaattta aggagatttt aatgaaaaga
     3721 tttttcacag cattatttgt ccttatcatc tgctcaatgc ttgcgggtca ggataaaaag
     3781 gcaacgctga cagtcgttct taaaggactt aaagtacagg gaactgttta tgtgagtttg
     3841 tatgacaagg ataacggatt cccgacaaag tcccagaatg ctgttaagaa cagtatcaaa
     3901 aaggttgccg ccgctacgga aaacatattg ttcaaagatc tccctttcgg agaatatgct
     3961 gtttcggtct ggcatgatga gaatggcaat ggagaaatgg aaaaaagctt tataggcatt
     4021 cccaaagaag ggatgggtgt ttcaaacgat gcaaagggta aaatgggtcc acccaaattc
     4081 aaagatgcaa aattcaatat tgacaaagaa gcaaagacta ttgagatcaa tgtaaaatat
     4141 taatctttag gaagttccat ttccttggga tagagttctt ttctgatcat ttggattatc
     4201 ttttcgtcgt gagtttcttt tttatatccc attacatagc tttttccgaa aatgaaaaga
     4261 gggactccgg ggctttcaat tttgaattcc tgtatctttt ttataagcag ttccttgtta
     4321 tcaaaatttc tgctcacttc aagcttctca aatttgatct caggatattt cttttcaagt
     4381 gatttaatga atggctctgc atgaacgcag tgcggacatg attctgaatg aaaaaaatag
     4441 aatacgggtt ttttggtatt tgatgccgag atcagaagaa ataccgacaa aaacaacaca
     4501 atctttttca cgattttatt ctccgaaata tatacaatta agctcaatgg tctgttttgt
     4561 gtcaaaccct tcatttaaaa taagttcggg tcttggtgat ttgtgttccc ctgatctgaa
     4621 aagagctttc gccgcttcgg ttcctgaagc cggataaaaa acctctgttc tgttctggcc
     4681 ctgtatctga agctgtgcaa tgtaatcaga tgcacctgtt acagccgaac tgttgatctt
     4741 tactgtcgtt gagtattctg tgatattgaa cgaagtgtcg acagttttat ctgaactcat
     4801 actgtgtgac acaagaagcg gtattctcat aaaacttttc ttttcttttt ctgaacttgt
     4861 gaagaacagg tcaagatagg cattgatcgt aagccccgat attacttctc cgtgccagtc
     4921 accatcggtc ggttttacaa tatcaataag atagtttgca gcagtcttgt cagggtttac
     4981 aacaatttga taatcattca gagttttgtt gttctgagcc cattctttga aaggtgtgcc
     5041 gtttatactg agacccagta cgatctttga agtggtaatg ccaagtgtct gtcccgacat
     5101 attacctgta acattcagat ttctttcaat atatgtcttg aactcgcttc caagaacccc
     5161 ttcaccttca taaataacat cgtattctcc tgttggaaca agaaatgaaa aggtgccgtc
     5221 actgagttcg gaaagattga tgacaggtgt ttcaattccg ttcaggtcac ggatctttat
     5281 caggtcatct ttttcagtag tgaatgctga accgttgagt gttacagtgc cctcaacaag
     5341 catcggtgtg atattgaaat tgatctcttt ttcaagcaca tactcttcag cgacattctg
     5401 gaatgtgaag tttgaaacag gaacgctttc actgtttaga tatttaagcg gaagggtgaa
     5461 tggtgaatat tcaaaagcgc ctttaacatt gttgaggtga ctttcaaagt tcatggtata
     5521 ggtcttcggg ttgtaaacca cttttgttcc gtcaggtgga ttgtcagggt cgggaacgat
     5581 attttttgtt cttcttacaa caagagcatc ataggtcaga tcttcccctg ttgtaccaaa
     5641 atcctttatc acgagtgatg aatagtcaaa tccggcaagc ttgagttttc ctctagtgca
     5701 gtctgcacct gtgcattctg atactgaagg aagtgatgcg ttgccaagga atgttactgt
     5761 tccatgcagt cttgcaaagt caaggatgat atcatgtgtc ttgcctgctg aaaggtcgtc
     5821 ccagagctca agtttgatgt attttacagg atagctgtct gtttcaggaa gatagaggac
     5881 attgaactgt gctcctttgg tcattgtgac tgaatatgct tttgtggcaa ggtcgggaat
     5941 gatcacagcg cttggattgt caaaaggagg attctcaacg attattatct gaccggtaga
     6001 aacatcatat ccttcgttta cggcatttcc gctgaaggtt acagtttcga cattgatacc
     6061 ggaatctaca tcttcttcta tctctattcc gtttgttgaa gaaaggatcg ttcctttgaa
     6121 aacgccctgt cctgatgcga gctgcccttc aaagtaaacc gaatatgtgc ccttggggac
     6181 tacgacatca aacgctccga aatctgaata agggatgatc ttttcgtaag tgcctgattt
     6241 gagtttaatc ttagttgcat cttccatgcc tgcttcaact gtgaaagcgg ctccgttttt
     6301 aaggactgag ccggtaaaat ggaagagagg aatctcgaag tcgagggttc tgtcacccga
     6361 agccatgtct atattttcaa gaagagtgat cctgttctca taagatgccg atttaaataa
     6421 aaaatcatat ctgccgacag ggatcttgaa ttttttgcct gaaagagttc cgtcaatgtc
     6481 tgctatcttg aattcgctca gagttgcttt gttctcaccc cagagttctc cggacaggtt
     6541 tgtgtcgtca ctttctccgt taatagttat ctcgcctata gtgagatttt ttgtctggac
     6601 atttatttca agcgttcccc ctgctttaac ttctgtgcag tcaccggtat aggaagtgct
     6661 gtcatcatca ctgccgcttt cgtcattttc agagtcatca tcagcaggat cggtatcgtt
     6721 cttcggatct tcatcgtaat tcacgatcgt gtctttatcc ggatttatca ccgagtcttt
     6781 ttcacattcc gatccattcc atgtgtatcc ttcgggacat ttgtcgtttt tgtcccctcc
     6841 gccacaggaa acagaaataa gaagtgctga aacaacgata aaaatcaaaa acaatctgtt
     6901 cattggaacc cccggcaatg ttgtcaacaa taaaattaca gttaattata gactgcttta
     6961 tatctttttc aataaataaa ttgaggattg atggtggttg aagagtttaa gggtttataa
     7021 tagtttgcag ttgaacagga taatattgac gcgtaaacgg gcagtacaga attttatcaa
     7081 cgattccgca aatgagaccg ctttcgctga tactgtccca ttcaatttca gcaatttttt
     7141 caggatagag gtcgtttgca tagtagcccg atgcgaagct tgagtaactt aggcagtaaa
     7201 ggtgtttgtc gttccttagt ccgcacaata tgtctcccac ggtacttatc tttttaaaat
     7261 cactgtaatt ccagactttt gtcggaatgt ttcgtagttt ccctgttaaa tttgcagtaa
     7321 tttgacccca gcagaaaatt tctccgcccg caattccgca agaggaatta tcacttgaaa
     7381 caacctgctc ccagtcggat ctgtcaccga ttttcaaagg ttcataaaca atttcgctgc
     7441 cccagcagta aagctctccc gattcgattc cgcaggtgtg tgtttcaccg gctgatactt
     7501 tttgccattc aattttgttc ccgatccttt gcggcatatc ataatagtaa gttgttccgt
     7561 ctccgatctg tccggaagaa ttacttcccc agcaatagag gtttcctccg gatgttccgc
     7621 aggtatgaga acagccgcat gatacggatt cccattttct atctgaaccg atttgtacag
     7681 ggctttctct atcttcagaa cttgaatcgc caacctgtcc taagttgttc ttcccccagc
     7741 agtaaagatc ttcatttctg atggcacagc tgtgataatc tccagttgaa acataatccc
     7801 agccagagct atcggttatt ttcataaatt cactgtaggt tcctgaatta actaagtaat
     7861 cgctgcaata tagactgcct cctgatatgc cgcatctttt ctgagttgct gtgcttatct
     7921 gtgtccattc atttaaggtg cctactttgg tgaggggatt tttttcttta ctttcaaaac
     7981 aatagaaatt acctgattca accccgcata aaccgaagca agtgagagca ctccagttgt
     8041 tcaaggtgcc gatttttgtc ggaatattga ctgattcaac agaactgtcg gcggtcagtc
     8101 cgatattccg tccccagcag aaaagctctc cccctgcaat tccgcaggta acagtgtgcc
     8161 atccgaaaat tttttcccag tcgtttctac tacctatttt tacgggctct gatcttgatt
     8221 caaaagttcc atctcccaac tgtccatagt cattatttcc ccagcagaaa agctcacctg
     8281 atgaaattgc acaataatac tcgtaatctt ctaaatatga gtacgaagtn nnnnnnnnnn
     8341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt cactccagtc acttcttgaa ccgatccgcg
     8461 ctatttctgt tttagataca gttgttccgt cgccgagttc tccgtttgca ttatatccca
     8521 tgcaatataa ttctcctgat gctattgcac agaaatatga taaagacggg tcattacttg
     8581 aaacagtaac tttactccag tcagtgcgat cactcatttt ttcagggtat ggactatctc
     8641 tccagcagtg cagttcgcca ctgccgttaa tgccgcattt gtaagtggct ccggagacat
     8701 cgatccatcc atctgatttg caatttattg aattttcaca tttattcaaa gagcatttgt
     8761 gctcaggttc ccatttgtta cagccgttat tattgacaca tgctgaaatt tttccgtcag
     8821 aacagacagt ctgaccctca aattcacatt cattagtgca cgaaagacag cttgcatcat
     8881 cggcacatac accgtctgaa catattacag ggaatgacca gtcgagaatg ccgttttcaa
     8941 gttctctgca aattcttgct gatccgctga cacattcagt cattcctgca aacagacagt
     9001 catccactgc acaaatgaaa tcatcagcac agattcctgt cagacatcct tcagattcaa
     9061 aaattagaca accatcgcta tttttcacac aggtgcttaa aattccttca ccacattttg
     9121 tctccccttc aaaaaaacat tcgttgacac attctttttc attttcagca cactcattaa
     9181 caaactgaac tgaaccatta agaactcctt ttaaaatatt ctttccttcg cttgctgttt
     9241 ctgttccttc gctttcttca aaatgccata ctgcaatagt ttgttcatca gcgtaaagtc
     9301 ttttctcagg tgtgaagttt tctgaatatc ttggaatgaa agaaaatctt atctcgtcaa
     9361 taagtcccga aaaatatgaa ggattgctgt ttaaccgcac gccgaaatag agactttcac
     9421 tgtttttgac aggagattta tcagcacttt cttttttctg aagaactccg tcaacaaaaa
     9481 gtttcagttc agtttcagat tttacaagtg caacatgata ccacttcatg ctttgaactt
     9541 cttttgatga atggagccac acatcttcat cactcgtggt tccgaactga tatccaccgt
     9601 ttggtgcact cgaaaatgta ctcaatgcat ctgagtcctt cccgtatatc cagtaagaag
     9661 gtgactctgt tgagctgcct ttcctgatca gaggatcctg attttcagcg ttaccctgca
     9721 ttgcccatgc ctcgatagtc catgtgttac caagatttaa agcatcatca tgttcaacag
     9781 aaatgtaaga atcaaccccg ttgaaataaa ccgatccgca cggcatactt tcatcagaat
     9841 cggcatcacc ggtttcatct gaaatctcat cagaatcaag atcaggaaac tgttcctcgt
     9901 caacatctga aaacgaatct tcatcattaa caggtctttc aaacttctga agaggatcac
     9961 atgagataac cataaaaagt gataataaaa taaaatactt catcattc
//
LOCUS       DAOOYB010000099         9966 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_7359_length_9966_cov_14.301584, whole genome shotgun sequence.
ACCESSION   DAOOYB010000099 DAOOYB010000000
VERSION     DAOOYB010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9966
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7359_length_9966_cov_14.301584"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(30..2657)
                     /gene="alaS"
                     /locus_tag="PL056_07250"
     CDS             complement(30..2657)
                     /gene="alaS"
                     /locus_tag="PL056_07250"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018624787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HPM46849.1"
                     /translation="MTTHWTIDTVRETFLNYFKQRGHAEVASSPLLPAEDKTLLFANA
                     GMNQFKNLFLGIEKRSYTRACSSQKCVRAGGKHNDLENVGFTTRHHTFFEMLGNFSFG
                     DYFKEDAIKYAWELVTEVFKIDKNRLYVTVFENDDEAEKIWVDIAGVDKKRIFRLGEK
                     DNFWAMGDTGPCGPCSEIHYDLGEQFKVEKPFHDNGMPDFDCGRFVEIWNLVFMQFNR
                     DESGNLSPLPNPSIDTGMGLERTTAILNGHLSNYEIDIFQSLKNFTRSVCPEKIPENL
                     ETSLNVIADHARSVSFLIADTITPSNEGRGYVLRRIMRRAIRHGHKTGFEDIFFDKVC
                     SKLIELMGKHYPELVEKKEFILNIVAEEEKRFRTTLEKGLSLLDHGISDAKKKGTKEL
                     SGSLVFKLYDTYGFPPDLTATILEEKGFVYNEKEYDAAMNEQKLRGRESWNANLDSKR
                     VSAINELLEEGITEPVFDGYVKEEADGRIVALFNNDFEKIDFIENGECFAVIDPVIFY
                     AESGGQVGDRGTISINGETVAVVDDCIKVNEFKIVHLNVTKKILAGKNVLQQNDRARR
                     GSIRKNHSATHLLHHALKSILGNHVNQAGSLVTPDRLRFDFNHFQQVTKEELKEIENE
                     VNWMIGRNFCVGTEVKSVDEAKKAGATALFGEKYGENVRVVSMGDSMELCGGTHVSST
                     GEIGLFKIVKEEGIAAGVRRIEAVTANNALAYFREMEETISEIADVLGIEKALVIKNV
                     EKLVDSNKSLQKEVKELMSRLSSIQASAITPLKEKEGTKIFVIDSGKNRTDALSLTDS
                     LKSKYDNALIVLKGTDSGKDILITASTGSAKEKFHAGNTLKLLLENFGGRGGGKPDMA
                     QGGAENIDFEKLKSLVENL"
     gene            complement(2762..3922)
                     /gene="metK"
                     /locus_tag="PL056_07255"
     CDS             complement(2762..3922)
                     /gene="metK"
                     /locus_tag="PL056_07255"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HPM46850.1"
                     /translation="MLRPYLFTSESVTEGHPDKVADQVSDAILDAILEKDRHARVACE
                     TFVTTGLVVVGGEITTNTYVDIPEIVRNTIIEIGYKDTKFCGFDGHTCGVLSTIDKQS
                     PDIAQGVNAGEGLFKEQGAGDQGLMFGYAIDENEDFMPTPINLAHQLVYRLAQVRKNG
                     EVPFVLPDGKSQVSVRYEDGMPVAVDKVVISTQHKDTVDYETIRSEIIEKVIKPVIPA
                     GLIHSGTEFFINPTGKFVVGGPVADTGLTGRKIIVDTYGGMGRHGGGAFSGKDPSKVD
                     RSASYMARYIAKNIVASGLAKKCEIQIAYAIGVAQPVSVYVNTFETSEIPEFEIEKAV
                     RKCFDMRPAAIIEQLDLLRPIYKKTAAYGHFGRDGFSWEKIDRTKDLKNLIR"
     gene            complement(4001..4387)
                     /gene="rpsI"
                     /locus_tag="PL056_07260"
     CDS             complement(4001..4387)
                     /gene="rpsI"
                     /locus_tag="PL056_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020887098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HPM46851.1"
                     /translation="MEQWYATGKRKTAVARVFLRPGKGEITVNGKTPKEYFYTDSNTM
                     KLDAPLNITKTKGKFSILVTVCGGGICAQAEAVRHGISRALVTYNPEMRPALKKEGFL
                     RRDERMKERKKYGLKSARARFQFSKR"
     gene            complement(4416..4847)
                     /gene="rplM"
                     /locus_tag="PL056_07265"
     CDS             complement(4416..4847)
                     /gene="rplM"
                     /locus_tag="PL056_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HPM46852.1"
                     /translation="MKLTRYTRSEDVKRVWFEISAEGLTLGRLATRVADVLRGKNKPH
                     YTPSVDCGDYVIITNAGKINMTGKKWTDKKYYSYSGYQSGMRELSAEKMVQKHPEHLI
                     MHAVKGMLPKNKLANQVIRKLKVYEGNEHPHTAQEPKKLEL"
     gene            5069..5767
                     /locus_tag="PL056_07270"
     CDS             5069..5767
                     /locus_tag="PL056_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mpv17/PMP22 family protein"
                     /protein_id="HPM46853.1"
                     /translation="MKKNDLVFLICIALFLAPFFVFPEVLKLYNDLNASNGMMMSFVK
                     FAVLATLGEVIGLRIKTGSYHQKGFGVIPRAIVWGFLGLGIKASFVIFGVGTPAFLSY
                     MGLKVSPATVNEAGFSMLKLVTAFSISVFMNLIFAPVFMTLHRITDMHIAETGGTLKG
                     FFSPIKFEKYFVSMNWKVQWSFVFLKTIPLFWIPAHTITFILPEQHRVLFAAVLGVVL
                     GVFLAIASLKGKKG"
     gene            complement(5771..7462)
                     /locus_tag="PL056_07275"
     CDS             complement(5771..7462)
                     /locus_tag="PL056_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HPM46854.1"
                     /translation="MVKRIFSFISALLIAFFIISCGETDCDDEGCGEIIEDEQTEYAP
                     EMTLRIISDTCAAETINSASIDKLGSLAITINNFKGELEFKKSFTKSEISSGMKVSGI
                     PDVDNATVILSGFGADPNVVKWSGKVIGLTFKKGKKTSISAVLYPTSGQSCFPFPLKN
                     PRFGHTATLLPDGRILVTGGFGICSASKCVANKSVEIIDVESGTVETLADMFEERAMH
                     EAVLLSDASVMIFGGVRALDIGTKNHDDYPALPYTFSIQATSVERYMPQYPKLNMRDN
                     NIGIPVENITQNMNLQYTATGMPFLPMQSYYVDSSVENRKTIYLVGGTDTSGVPTNKV
                     YAFDVFETAEGITLSASREIAPVDKDKILMPAVGSFGGSLFSAGGRIKEPVSVASFYT
                     ADSSTAWEGTGPNLFYTKGIMVDNNLYTFGGLENKGEDALANNLKGYKWSIGEKTSSA
                     TTNNLLTYGSSLFFADAVYHEAKGNFIVVGGAGGSKTDISQGNNLYQVISKSTFSVFK
                     EPLSYVTKYKRILPRAVIIPGGTVTNDDTIFITGGTTAIGSSGVAVEVIEINNLK"
     gene            complement(7465..8001)
                     /locus_tag="PL056_07280"
     CDS             complement(7465..8001)
                     /locus_tag="PL056_07280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HPM46855.1"
                     /translation="MKVLVISDTHGNLPISIEKIRADAVIHAGDVGDRKFLSRFSNIE
                     KFYAVAGNTDSILEDHIPQTICDEIEGVKFYVVHNLSAPHRIIMSNYREIKECSPDIV
                     IYGHTHTPLIEEKDGVVYLNPGSLGKEGLTGHRSYAVLEIEGSKIVSMNIFDADSGAL
                     LSSKKENCLKNKNFEKEN"
     gene            complement(8068..9810)
                     /locus_tag="PL056_07285"
     CDS             complement(8068..9810)
                     /locus_tag="PL056_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006919120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPM46856.1"
                     /translation="MKILNIVDVNIVKIVLTKYYIAKNLEYDFIYPELEAVKKRISEY
                     KPDILVVQSNLMYTDVAGFLNMLNMDIDSDVIVFMIREDADQQEELSVSLAKYRSVHF
                     AGKENFYELFDSLLPSELTKKAPVPDEEITSDKRTILYVDDSPVMHHFVRTALKDSGF
                     NLIEANDGQEGYEKYCKYLPHIIVTDIEMPKMNGLELCRKIKENNEGRFIPVVILSSK
                     SQPVDIDTAFNYGADDYLVKPVSPDNLIEKVKDYFAVLDRKKRNRILVVDDSKVSAEM
                     ISHALIKNSHNVLIASNGETAYDMALKEKPEIIITDVEMPGMNGYDLVKRLRELPELK
                     DVSVIMMSSRDRKSDIKKSEKLGVARYFIKPFDIEKLVIVVEQLLLEKYNIYKKEYEY
                     MLSTIKALVTALEARDEYTKGHTFRVSKYSLMLGKQMGLTTFELDKLEIAANLHDIGK
                     IGVRDDILLKPGRLSDEEYAKIQEHAVIGAEILRPLNSLNDVVPLILFHHERWDGNGY
                     PTMISGIEIPLGARIIAIADTFDAITSDRPYRKALSEEKAIEIIKENSGTQFCPKCSA
                     AFLEMAKNRKILSE"
BASE COUNT         2615 a         2119 c         1951 g         3281 t
ORIGIN      
        1 tgcccttttg aagagtaatt ccgctgaggc tataaatttt ccacaagact tttaagtttt
       61 tcaaaatcga tattttcagc tccgccttga gccatgtcag gttttccgcc gcctctgccg
      121 ccaaagtttt caagcagcag tttcagtgtg ttgccggcgt gaaacttttc ttttgcactg
      181 cctgttgaag cggtaatgag gatgtctttt cctgaatctg tcccttttaa aactatcaga
      241 gcattatcat attttgattt cagcgaatcg gttaaagaga gtgcatcagt tctatttttt
      301 cctgagtcaa ttacaaatat ttttgttcct tctttttctt tgagcggtgt aattgccgat
      361 gcctggatcg aagaaagccg gctcatcagt tctttaactt ctttctgaag agatttgttt
      421 gaatcaacaa gcttttcaac atttttaatc acaagtgctt tttcaattcc gagaacatct
      481 gcaatttcag aaatggtctc ttccatttcc cgaaaatatg caagggcgtt atttgctgtc
      541 actgcctcaa ttcttctgac tccggcagca attccttctt ctttcactat tttaaaaaga
      601 cctatttcac cagttgaaga aacatgtgtt cctccacaaa gctccattga gtcacccatt
      661 gaaacaaccc tgacattttc gccgtatttt tcaccgaaaa gagcagtagc tcccgctttt
      721 ttagcttcat caaccgattt aacctcagtt ccgacacaga aatttctgcc gatcatccag
      781 ttcacttcat tttctatttc tttaagctct tcttttgtca cctgctgaaa atgattgaag
      841 tcaaaacgga gccggtcggg agttacaaga gatcctgcct gatttacatg atttccaaga
      901 attgatttca gagcgtgatg aagaaggtga gtggcagagt gattttttct tatcgagccc
      961 cttctggcac ggtcattctg ctgaagaaca tttttccctg ccagaatttt ttttgttaca
     1021 ttgaggtgaa caattttaaa ttcgttgact ttaatacagt cgtcaacaac tgcaacagtt
     1081 tcaccgttta ttgaaattgt tcctctgtct ccaacctgtc cgcccgattc agcataaaat
     1141 atcacaggat cgatgacagc aaagcattcg ccattttcaa taaaatcaat tttttcaaaa
     1201 tcgttgttaa aaagagcaac aattctgcca tctgcctctt ctttgacata tccgtcaaaa
     1261 accggttcgg ttataccttc ttcaagaagt tcatttattg ctgaaaccct ctttgaatcg
     1321 aggtttgcgt tccacgactc tctgcctcgg agcttctgct cgttcattgc ggcatcatac
     1381 tctttttcat tgtagacaaa acctttttct tcaagaattg tggcagtgag atccggcggg
     1441 aaaccgtaag tgtcataaag tttgaaaaca agagaccctg aaagttcttt tgttcctttt
     1501 ttctttgcat cgcttattcc gtgatcaaga agcgaaagtc ctttttcaag agttgttctg
     1561 aatctttttt cctcttctgc gacaatattt aaaatgaatt cttttttctc aacaagctcg
     1621 ggataatgtt tccccatcag ctcgatcagt ttggagcaga ctttgtcaaa gaaaatatct
     1681 tcaaaccctg ttttgtgccc gtgtctaatc gctcttctca taattctgcg cagaacatat
     1741 ccgcgacctt cgtttgaagg agtgatggta tctgcaatga gaaatgatac gcttcttgcg
     1801 tggtcagcga tgacatttaa gcttgtttca agattttcag gaattttttc aggacagacc
     1861 gaacgggtaa aatttttcaa agactgaaaa atatcaattt cgtaattaga aagatgcccg
     1921 ttcaatattg cagtcgttct ttcaagcccc attcctgtat caatgctcgg attgggaaga
     1981 ggtgagagat ttcccgattc atcgcggttg aactgcatga aaacaagatt ccatatctca
     2041 acgaacctgc cgcagtcaaa gtccggcatt ccgttatcat gaaacggttt ttcaactttg
     2101 aactgttcgc cgagatcata atggatctca ctgcacggcc cgcacggtcc tgtgtcaccc
     2161 attgcccaga aattgtcttt ttcgccaagc cggaaaattc ttttcttatc cacgcccgca
     2221 atgtcaaccc atattttctc cgcttcatca tcgttttcaa aaacggtgac ataaaggcgg
     2281 ttcttatcaa ttttaaaaac ttcagtgaca agctcccatg catattttat ggcatcttcc
     2341 ttaaaataat caccgaatga aaaattgccg agcatctcaa aaaaagtgtg gtgacgggtt
     2401 gtaaaaccga cattttcaag gtcgttgtgt tttcctcccg ctcttacgca tttttgtgaa
     2461 ctgcatgcac gggtataaga tcttttttca attccaagaa aaaggttttt aaactggttc
     2521 attccggcat ttgcaaaaag cagcgttttg tcttcggcag gaagaagagg cgaacttgca
     2581 acctcagcgt gtcctctttg cttaaaatag tttaaaaaag tttcccttac agtgtcaatt
     2641 gtccagtgag ttgtcatcat aatctccaat aaattcaaaa aactgccgga attctatagt
     2701 tctttcgatt tttctcaaga aaaaaacaca agggaagtgt tgaagaaaaa aaagaaaaat
     2761 gttatctgat aaggtttttg aggtcttttg ttctgtcgat cttttcccat gaaaaaccgt
     2821 ctcttccgaa gtgaccgtaa gctgcagttt tcttgtagat cggtctcaaa agatcaagct
     2881 gttcaataat tgcggcaggt ctcatgtcaa agcattttct gacagctttt tctatttcaa
     2941 attccggaat ttcagatgtt tcaaaagtat tgacatatac agataccggc tgtgctacac
     3001 ctattgcata agctatctgg atctcgcatt tttttgcaag acctgaagcc acgatgttct
     3061 ttgcgatgta tcttgccatg tatgaagctg atctgtcaac cttggaaggg tctttacctg
     3121 aaaaagctcc gccgccgtga cggcccattc cgccgtatgt gtcaacaatt atctttcttc
     3181 ctgtaagtcc agtgtcggca accggtcctc cgacaacgaa tttacctgtc gggttaataa
     3241 aaaattcggt tccggagtga ataaggcccg ccggaataac cggttttatg actttctcga
     3301 tgatttcact tctaatggtt tcataatcaa cggtatcttt atgctgtgtg ctgattacga
     3361 ctttatcgac agcaacaggc attccgtctt catagcggac agatacctga gattttccgt
     3421 caggaagaac aaagggaacc tcaccgttct ttcttacctg agcaagacgg taaacaagct
     3481 ggtgggcaag atttatagga gtaggcataa aatcttcatt ttcatctatt gcataaccga
     3541 acataagacc ctggtcacct gcgccctgtt ctttgaaaag cccttctcct gcgtttacac
     3601 cctgcgcgat atcgggagat tgtttgtcaa ttgtggaaag aacgccgcat gtgtgtccgt
     3661 caaaaccgca gaactttgtg tctttgtagc ctatttcaat aatggtgttc cttactattt
     3721 cgggaatgtc gacataagtg tttgtcgtta tttctccacc aactaccaca agtcctgttg
     3781 tgacaaaggt ttcacaggca actcttgcgt gcctgtcttt ttcaaggatt gcatcaagaa
     3841 ttgcgtcgct gacctgatct gcaactttgt ccggatgtcc ttcagtaacc gattccgagg
     3901 taaaaagata gggtctcaac atttttggca cctcctaata caaaaaaacc ccctcagata
     3961 gagagggggt tgaaacaata taacaaagaa agcgagaaat ttatctcttg gagaactgga
     4021 atctcgccct tgcggacttc aatccgtatt tctttctttc cttcattctt tcgtctctcc
     4081 tgaggaaacc ttctttttta agagccggtc tcatttcagg attatatgta acaagtgctc
     4141 tggaaattcc atgccttact gcttcggcct gagcacagat gcctccacca cagacagtta
     4201 caagaatgga aaactttcct ttagtcttgg taatgttgag gggtgcatca agtttcatgg
     4261 tgttgctgtc tgtgtagaaa tactctttgg gagtttttcc gttaacagta atttcgcctt
     4321 tgccgggtct taaaaagact cttgcaactg cagtttttct tttgccagta gcgtaccact
     4381 gttccatcaa ttatctccaa cagttaaaca ataaatcaaa gttccagttt ttttggttcc
     4441 tgtgctgtgt gaggatgttc atttccctca taaactttca gctttctgat aacctggttt
     4501 gcaagcttgt ttttggggag cattcccttg acagcgtgca taatgaggtg ttcagggtgt
     4561 ttctgaacca ttttttctgc ggaaagttct ctcattccgc tctgataccc ggagtagctg
     4621 tagtactttt tgtcagtcca ttttttaccg gtcatgttta ttttgccggc attagtaatg
     4681 ataacatagt caccgcagtc aacactgggc gtgtaatgag gtttgttctt tccacgcaga
     4741 acatctgcaa ctcttgtggc aagtcttccc agtgtaagcc cttctgcgct gatctcaaac
     4801 cacactcttt ttacatcttc acttcttgtg tatcttgtca atttcatagt gcttcctttc
     4861 ctttataaag ttagcaaaaa agcgttgcac atcctacatg tgttatatgt gttgtcaaga
     4921 attttttgtt taataaaaga aaacgcgaat aattataata tgttgcgtag ccaaagcaat
     4981 cttatgtttt taaaaatttg acgcttggct caaattacaa tagattgcca tggcttaaag
     5041 ttttataact tccagacgag gatagatcat gaaaaagaac gaccttgtgt ttttaatctg
     5101 cattgctttg ttccttgctc cgttttttgt ttttcctgaa gtgctgaagc tttataatga
     5161 tctgaatgct tcaaacggaa tgatgatgag ttttgttaaa tttgccgttc ttgcaacact
     5221 cggtgaggtg atagggctca gaataaagac cggttcctac catcagaaag ggtttggtgt
     5281 aattccaaga gcgatcgtgt gggggtttct gggtctcggc ataaaagcat cttttgttat
     5341 tttcggagtg ggaacgccgg ctttcctttc atatatggga ttaaaagttt ctcctgcaac
     5401 agtgaacgaa gcagggtttt caatgcttaa gcttgttaca gctttttcta taagcgtgtt
     5461 catgaatctc atattcgcac ctgtttttat gacgcttcac aggataacag acatgcacat
     5521 tgctgaaaca ggcggaactt tgaaaggttt tttttcgccg ataaaatttg aaaaatattt
     5581 cgtgtcgatg aactggaaag tgcagtggag ttttgtattt ttaaaaacga tcccgctgtt
     5641 ctggatacct gcacacacca taactttcat tcttcctgaa cagcacagag ttctttttgc
     5701 cgcagttctc ggtgtggttc tgggtgtttt tcttgcgatc gccagtctaa agggaaagaa
     5761 aggatagaaa ttatttcaga ttatttatct caattacttc gacagccacg ccgctgctcc
     5821 cgatagcagt tgttccgccg gtaataaata tcgtgtcatc atttgtgact gttccgccgg
     5881 gaatgataac ggctctggga agtattcttt tatattttgt aacatatgaa agaggttcct
     5941 taaacactga aaaagtgctt ttgctgataa cctgataaag gttgttcccc tgtgaaatat
     6001 ctgttttgct tccgccggca ccacccacaa ctatgaaatt tccctttgct tcgtgatata
     6061 ccgcatcggc aaaaaagagc gaagaaccgt atgtcaaaag attgtttgtc gtcgcagaag
     6121 aagttttttc tccgatcgac cacttgtatc ccttcaagtt atttgcaaga gcatcctctc
     6181 ctttattttc aagtccgccg aaagtgtaga gattgttgtc aaccattatt ccttttgtgt
     6241 aaaaaaggtt gggaccggtc ccttcccatg cggttgagct gtcagcggta tagaaacttg
     6301 cgacagagac cggttcctta attcttccgc ctgctgagaa aagagatcct ccgaaagatc
     6361 cgactgccgg cataagtatt ttgtccttat caacaggagc tatttccctt gaagcgctca
     6421 gtgttattcc ctcagcagtt tcaaaaacat caaaagcgta aactttattt gtcggtactc
     6481 ctgatgtatc tgttcctccg acaagataaa tggtctttct gttttcgaca gagctgtcaa
     6541 catagtaact ctgcatgggg agaaaaggca ttcctgtcgc tgtatactga aggttcatat
     6601 tctgagtgat attttcaacc ggtattccga tgttgttatc tctcatattg agtttcggat
     6661 actgaggcat gtatctttca acggaagttg cctgaattga gaaggtatag gggagtgccg
     6721 gatagtcatc atggttcttt gttccgatgt caagagctct tacgccgccg aagatcataa
     6781 cgcttgcgtc agagagcaga actgcttcat gcattgctct ttcctcaaac atgtctgcaa
     6841 gggtttccac ggttcctgat tcaacatcga ttatttcaac agatttgttc gcaacgcatt
     6901 ttgatgctga gcatatcccg aatcctcctg taacaaggat ccttccgtcg ggaagaagag
     6961 ttgctgtgtg accgaatcgc gggtttttaa gcgggaacgg aaagcatgat tgacctgatg
     7021 tcggataaag aactgcgctg atcgaagttt ttttcccttt cttaaaagtg agaccgatta
     7081 cttttccgga ccatttaaca acattaggat ctgcaccgaa gccggaaaga attacagttg
     7141 cattatcaac atccggaatg cctgaaactt tcatgcccga actgatctcg ctttttgtaa
     7201 atgatttctt gaattccagt tcacctttaa aattgtttat tgttatggca agactgccga
     7261 gtttatcaat gctggctgag ttaatagttt cggcagcaca ggtgtctgaa attattctca
     7321 gcgtcatttc cggagcatat tctgtctgtt catcttctat tatttcaccg caaccctcgt
     7381 catcacagtc tgtttcgccg caggaaatga taaaaaaggc gatgagcaaa gcagaaataa
     7441 atgaaaaaat tcttttaacc aaaatcaatt ctccttttca aagtttttat tttttaaaca
     7501 gttttctttt tttgaactca gcagagctcc tgaatcggca tcaaatatat tcattgaaac
     7561 gatcttcgat ccttctattt ctaaaacagc atatgatctg tgacctgtaa gcccttcctt
     7621 gcccagagat ccgggattga ggtatacgac gccgtctttt tcctctatca gcggagtgtg
     7681 tgtgtgtccg tagatcacta tatcgggcga acattctttt atttctctgt aattggacat
     7741 tattattctg tggggggcac ttaagttgtg aaccacataa aatttaaccc cttcaatttc
     7801 atcacatatg gtctggggaa tatgatcttc cagtattgaa tcggtgtttc ctgcaacggc
     7861 atagaacttt tcaatattgg aaaatctgga aagaaatttt ctgtcaccga catctccggc
     7921 atgtatgaca gcgtcggctc ttattttttc aatggatatg ggcagattgc cgtgagtatc
     7981 tgatatgacc aggaccttca taagaacatc gcctcaaaaa aattggttat atatatcacg
     8041 ggttttgtaa tatgtcaaaa ataggggcta ttcagaaagt attttccggt ttttcgccat
     8101 ttccagaaat gccgcggagc atttcggaca gaattgtgtg cccgaattct cttttattat
     8161 ttcaatggct ttttcttctg aaagggcttt tctgtaaggt ctgtcggatg ttatggcatc
     8221 aaaagtgtct gctatggcaa ttattcttgc tcccagaggt atttcaattc cgctgatcat
     8281 tgtggggtat ccgttgccgt cccatctttc atggtgaaaa aggataagcg gaactacatc
     8341 attcaatgag ttcagcggtc tcaatatttc tgctccgatg actgcatgtt cctgaatttt
     8401 ggcatattct tcatcgctga ggcggcccgg tttaagaaga atatcgtccc gcacaccgat
     8461 cttgccgata tcatgaaggt ttgccgctat ttcaagtttg tcgagctcaa aagttgtcag
     8521 ccccatctgt ttgccgagca ttaaagaata ttttgatact ctgaaagtgt gcccttttgt
     8581 gtattcatct cttgcttcaa gagcggttac gagcgccttg atcgttgaaa gcatgtattc
     8641 atattctttt ttatagatat tatatttttc aagcagaagc tgttccacga caatgaccag
     8701 tttctcaatg tcgaaaggtt tgatgaaata tcttgcaaca ccgagttttt cgcttttctt
     8761 tatgtcgctt tttctgtcgc gactgctcat cattataaca gaaacatctt tcaattcggg
     8821 cagttcgcgc aatcttttca caaggtcgta tccattcatt cccggcattt caacatcggt
     8881 gatgattatt tcaggttttt cctttaaagc catatcataa gccgtttcac cgtttgaagc
     8941 tatcagaaca ttgtgactgt tctttatcag tgcatgggag atcatttcag cagagacctt
     9001 tgaatcgtca acgaccagga tcctgttcct tttttttctg tcaagaaccg caaagtagtc
     9061 tttaactttt tcgataaggt tgtcaggtga tacaggtttt acgaggtagt cgtcagctcc
     9121 gtaattaaaa gcggtgtcga tgtcgaccgg ctgagatttg cttgaaagta ttacaacagg
     9181 aataaagcgt ccttcgttat tttcctttat cttcctgcaa agttcaagtc cgttcatttt
     9241 cggcatttca atatcggtga cgattatgtg cggaagatat ttgcagtatt tttcatatcc
     9301 ctcctgaccg tcatttgcct cgataaggtt aaaccctgaa tctttaagag ccgttcttac
     9361 aaagtgatgc attacaggac tgtcatcgac ataaagaatg gttcttttgt ctgaagtgat
     9421 ctcttcatcc ggaacaggcg ccttttttgt tagttctgaa gggagcaggg agtcaaaaag
     9481 ttcatagaaa ttctctttgc cggcaaaatg gacgcttctg tattttgcaa gtgaaacgga
     9541 aagttcttcc tgctgatcag catcttcccg tatcataaaa acaatgacat cagagtcgat
     9601 atccatgttg agcatgttca gaaatccagc cacatctgtg tacatcagat tgctttgaac
     9661 aacaagaatg tcgggcttat attcagagat ccttttcttt acagcttcaa gctcaggata
     9721 gatgaaatca tattcgaggt tctttgcaat atagtatttt gtaaggacga tctttacaat
     9781 gtttacatct acaatgttga gtattttcat cacaccttct caaacaacca ccgacattat
     9841 ataaagtttg tctacttttt caagcaaaaa acaacataaa attatacaat ttaagggcaa
     9901 acccgccatt ttttttaaac ataatgttgt tgtgtgtgta aaaaattaaa aaattgactg
     9961 aaatct
//
LOCUS       DAOOYB010000100         9931 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_7383_length_9931_cov_9.065614, whole genome shotgun sequence.
ACCESSION   DAOOYB010000100 DAOOYB010000000
VERSION     DAOOYB010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9931
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7383_length_9931_cov_9.065614"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            189..1439
                     /locus_tag="PL056_07290"
     CDS             189..1439
                     /locus_tag="PL056_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46857.1"
                     /translation="MKRFFIAVILISSLIFTVSCGDNDTGDSGDTGNTGDTGDTGDTG
                     DTGDTGDTGNTGDTGDTGNTGDTGNTGNTGDTGNTGNTGDTAVECVGISIESMALLQG
                     EDMYEGTLSKQIGDETLEDIISFQFYTEDYSPVPELPAGTYDLGAGVNANFNSCSECV
                     LIFEDDYSKTYFQQSGTLTITENKAGTLETKGTLTAKLVEVTIADDYTSTPVAGGGCF
                     EIEAGTWDIICIPDCEGKICGSDGCGGECGDGCEIDKQCNAEGTACVNYECTAITLGE
                     TSAGVISAEDEVWTYDVLFDPAAGDAELDDKVSLQLYAEVEAEDSFNLAGTNYYDCEQ
                     CILVYEDMEFDETSGDLTAIGKVYFQQKGTLDFESFDAATGKVKASVTGLRLIEVTID
                     SEYTSYPVQGGACLEVATGTVELK"
     gene            complement(1465..3828)
                     /locus_tag="PL056_07295"
     CDS             complement(1465..3828)
                     /locus_tag="PL056_07295"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM46858.1"
                     /translation="MMKQNDTYNQEKCPVTGRLIVTLPEFADVRINKDYHTTLQKIGE
                     NIVYVQSRGSLKNVDLDKFNMLVEEFCAAAGVKKPFVQIRNLSELTGRLSFKNMKKQM
                     YYFNEHRATMTGIIFIEEPSWFRPFINQGLRFFGPTSFKVASAKNYHEAVKAAVTILE
                     QKNEKPKEDQMAISFSDIIFKPEWVYENPQNNYKYQVGYIPGVLFYVSMHGNLTTIDD
                     VQKSSDLATKVLVENHLRKLPYIITNFSELAAIRSFRCRQQFAKEVKKRMQETDNNGG
                     ARIIINPDNFNRALITAFSPFLNIRYIITNSVEETFKKINNSPMELKSAREEKPMIVT
                     PAHLEELSNVFGELQWEESGVIPNAVVSKDNPLAYLTESIALIRNDLNELRQNERKIQ
                     AEREQELRKAKELAEAASNAKSEFLAKMSHEIRTPLNGIIGFTDLLKDTPLTQLQQQY
                     VDNVNVSGHTLLGIINDILDFSKIEAGMMELDPVITDMYELLKNSIEIIRFSAERKGL
                     EIKMIIDGSMPEFAKVDPVRLKQILANLLSNAVKFTETGEVGLEVKFNYTAGKTGRFT
                     FYVRDTGIGINDDQKAKLFKAFSQADSSITRKYGGTGLGLIISEMIANKMGSKINIES
                     VPGKGTTFYFEISVDVFKESTDMKMESENESVKEQVVSERSLSLLIVEDNSVNMVVIK
                     AVVGKMLPNARLIEAANGIEAVENFRKYSPDLIFMDIQMPKMDGLEATEKIREIESVS
                     KTRVPIIALTAGVEVSEQKKCMKSGMDHFLAKPLEQEKIRKVLKNFL"
     gene            complement(3812..5134)
                     /gene="trmFO"
                     /locus_tag="PL056_07300"
     CDS             complement(3812..5134)
                     /gene="trmFO"
                     /locus_tag="PL056_07300"
                     /inference="COORDINATES: protein motif:HMM:NF003739.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HPM46859.1"
                     /translation="MKIVILGAGLAGSEAALQFAQNGFSVELIDCKPHFLNEEVYSEP
                     SPAELVCSNSFKSESPSTASFLLKEEMREMGSFLLKAASECSVPAGASLAVNRVEFSK
                     LISKMIDENGCITFHKGLNISTVEELKARFQGDFYIIATGPLTDKIMMNSISAEGGSF
                     YDAIAPLVTLDSIDLTECFWGSRYGKGDPDFLNIPLDKDEYAQFVEALITAEKIPWNS
                     IEKPEFFERCMPIEVMAERGFKTLSFGPFRPVGFEWKGRRPYAVVQLRAENSEKSVLN
                     LVGCQTRMKQGEQKRVFRTLPGLKNAEFTRFGSVHRNSFFNAPIVLENGVKVRTMENV
                     FVAGQLSGVEGYNESIFSGMWAALCIAEITKGNNIKDILPPKETMTGGILRKMSAVTN
                     DFQPVNANFALIDNPNTLGKKIRKEYYVADGLKRFKEWFKIKHNDETE"
     gene            complement(5131..7356)
                     /gene="topA"
                     /locus_tag="PL056_07305"
     CDS             complement(5131..7356)
                     /gene="topA"
                     /locus_tag="PL056_07305"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HPM46860.1"
                     /translation="MKLLIVESPTKAKTLTKYLKGFKVVASMGHIIDLPSKRLGVDIE
                     NNFKPRYEVIPGKEKVIAMLKKEASEAEEIYIGSDPDREGEAIAFHIASLFEGREVRR
                     VLFHEITKTGVLKAIDVFSKLNDNLYNSQQARRILDRIVGYKLSPFLWRSIKKGLSAG
                     RVQSVALQMVAEREKEIKAFVPEEYWNFKADFKVKAGKVDALLDKIKGEKVSVADGLK
                     AEEIKKEIISAGAFSVTSVEKKEKKESPLPPLNTSNLQQEASRLYNYTAEQIMRIAQS
                     LYEGKDLGEEGVTGLITYMRTDSFRISEEANAALRNYISDNIGKNYLSPKTRVFANKK
                     GKTQDAHEAIRPTSVALTPDKVASKLTKDELNIYTLIWRRFVATQMQDAVYETTVITI
                     EDSNRKFLFKKTGSVIQFEGYRKIYKKNGREDLTPEVLQGESAELNKVDAEQHFTKPS
                     PRFTEGSLVKELEEQGVGRPSTYATVIKQIVQRNYIERMKDPKGALMATELGILVSDT
                     LVSFFPEIINIGFTAGMESELDTVETGDRDWLDVVQSFYGTFNKVLDSGISKVKKEGK
                     KRIYAEEACPKCGKKLVVRWSKKGNQPFLSCETFPDCDFAGSIEKVGEEVHLKSVEQS
                     ADRKLDEKCPNCGSPLVVKNGKFGEFKACSAYPKCKTIIREEKFVAACPKEGCDGKIT
                     PKRSKKGKAFYGCTNYPKCDFVSWYPPVAGEKCEKCGFAYIVEKKRSGKVCQNCGHKP
                     K"
     gene            complement(7353..8255)
                     /locus_tag="PL056_07310"
     CDS             complement(7353..8255)
                     /locus_tag="PL056_07310"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HPM46861.1"
                     /translation="MMKIDAGDNRHPDKILKCRPEIAYPLYIEGSLIPEDAGGVAIVG
                     SRKPSLYGIKMAQAVAQKAVMNSKTVISGLARGIDTESHRSALKYGGRTIAVLGTGID
                     VIYPEENIGLAEEIRQNCALISQFPPQTPPLKKNFPIRNEIVAKMCSVLVLVQASTKS
                     GSLITARLAREFDKKVFVIPGPIDDELFTGNYQFLNSRKKDPGVELLYDLEQLDEFFN
                     CKNFFSEHIKPLNVLTDTDLEDDEKKVYRLIVESGEGIDFDTLSERSGFSATELPSIL
                     LFLMMKEMIDELPGKIYRYSEVKL"
     gene            complement(8252..9145)
                     /gene="ispH"
                     /locus_tag="PL056_07315"
     CDS             complement(8252..9145)
                     /gene="ispH"
                     /locus_tag="PL056_07315"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HPM46862.1"
                     /translation="MKVIVAENSGVCFGVRRALDLLEESVKTAELEKRKAVMLGPLIH
                     NPRVVEHYASIGVEVRDTENIPESCIVVVRSHGITLDAENKLEKIQGLTVVDTTCPYV
                     KRIHQLVEKKSSGGYAVIVMGDKDHSEVQGITSRIKGHFLVVSPAEVSQKWSEVEEFI
                     TEHRKIYCVAQTTSRPANYDNMLRKIRKFIEQKKENIFESAQTICNATFSRQDSAREL
                     ATRVDAMIVLGGMNSSNTMKLFQVVKEKNEKSYMVESVDDFTEEMLNELKKCSVAGLT
                     AGASTPDDQILEMKNFLESLK"
     gene            complement(9142..9816)
                     /gene="cmk"
                     /locus_tag="PL056_07320"
     CDS             complement(9142..9816)
                     /gene="cmk"
                     /locus_tag="PL056_07320"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HPM46863.1"
                     /translation="MKKIRIAIDGPAGAGKSSVSRMVAGLLGYQYVDTGAIYRSLAYA
                     MNETVKLEDHAGIRQLLDNFDVSFSMEDDVNHIFLNGEDITPFIRTEKVSMLASSVSA
                     LSFVREALLEMQKRYARAGGVVMEGRDIGTVIIPDAELKIFLTASPEKRAARRMLELK
                     AKGVEITMENLVEEIKRRDDQDSKRAIAPLKIAVDGVLVDNSDIDLFETAELIVKYAR
                     EREKLN"
BASE COUNT         2604 a         2306 c         1852 g         3169 t
ORIGIN      
        1 gatgtctgca caactttttc aacgataaat gcgattgctg aacttctaaa aaaaaacgga
       61 gctgaaagtg taaacgcagt tacacttgcc agaacttccc catattttac ctgatctttc
      121 tttttaattt cattttgtct gtctattgat gtataattac tggctaacaa tttcttttgg
      181 aggattttat gaaaagattt tttattgcag ttattttgat cagttcacta atttttactg
      241 tttcatgcgg agacaacgat accggcgatt ccggtgacac aggcaacaca ggtgacacag
      301 gcgacaccgg tgacacaggc gacaccgggg acacaggtga taccggaaac actggtgata
      361 ccggcgacac gggcaatact ggcgacacag gcaatacagg aaataccggt gacaccggaa
      421 acactggaaa caccggcgac acagctgttg agtgtgtagg aatttcaatc gaatcaatgg
      481 cattgcttca gggagaagat atgtatgaag gaactctttc aaagcagatt ggagacgaaa
      541 cccttgaaga cattatatct ttccagttct acacggaaga ttattctcct gtgcctgagc
      601 ttcctgccgg aacttatgat cttggagcag gtgtcaatgc aaattttaat tcctgttccg
      661 agtgtgttct gatctttgag gacgactact caaagaccta ttttcagcag agcgggacac
      721 ttacgatcac agaaaacaaa gcaggaactc ttgaaacaaa aggaactctt actgccaaac
      781 ttgttgaagt cacaatcgct gacgattaca catcaacacc tgttgcaggc ggcggctgtt
      841 tcgaaatcga agccggaaca tgggacatca tctgtattcc tgactgtgag ggaaagatat
      901 gcggcagcga cggttgcggc ggtgagtgcg gtgacggttg tgaaatagat aaacaatgca
      961 atgctgaagg taccgcctgt gttaattatg aatgcacagc aataacactc ggggaaacat
     1021 ctgccggagt cataagtgca gaagatgaag tttggactta tgatgttctt tttgatcctg
     1081 ctgccggtga tgcggaatta gacgacaaag tgtcgctgca gctttatgcc gaagttgaag
     1141 cagaagattc atttaatctt gccggcacaa attactatga ctgtgaacag tgcattcttg
     1201 tttacgaaga catggaattt gatgaaacca gcggagacct tacagcgatc ggcaaagtat
     1261 atttccagca gaaaggaacc cttgattttg aatcatttga tgccgcaaca ggaaaagtta
     1321 aagcatctgt aacaggactt agacttattg aagtcacgat agacagtgaa tatacttcct
     1381 accctgttca aggtggagca tgtcttgaag ttgccacagg aactgttgaa cttaagtaac
     1441 tgaattctat tcgttctgac tgatctaaag aaaattcttt aacactttcc tgatcttttc
     1501 ctgttcaagc ggttttgcca gaaagtgatc cattcccgat ttcatgcact ttttctgctc
     1561 agaaacttca actcctgcag taagagcaat tattggaacg cgggtttttg aaacagactc
     1621 tatttccctt atcttttcag tcgcttcaag accatccatt ttcggcatct gaatatccat
     1681 aaatatcaga tcaggtgaat attttctaaa attctcaacc gcttcgatac catttgcggc
     1741 ctctatcaga cgggcgttgg gcagcatttt gccgacaact gccttgatca cgaccatgtt
     1801 cactgaatta tcttcaacaa tcagaagaga aagagatcgt tcggacacaa cctgttcttt
     1861 aacactttca ttttcagatt ccattttcat gtccgtactt tccttgaaaa catcaactga
     1921 tatttcaaag taaaaagttg tccctttccc cggaacactt tcaatattta tcttacttcc
     1981 cattttatta gcgatcattt ctgaaataat gagcccgagc cccgttccac cgtatttacg
     2041 ggtaatagag ctgtctgcct gtgaaaatgc cttgaaaagt tttgctttct gatcatcatt
     2101 tattccgatt cctgtgtctc taacataaaa agtgaatctt cctgtttttc cggctgtata
     2161 attgaattta acttccagcc caacttcacc ggtttcagta aatttgacag cattacttaa
     2221 aagatttgca aggatctgct tcaacctgac aggatcaact tttgcaaatt caggcattga
     2281 gccgtcaatg atcattttta tttcaagccc tttcctttca gcagaaaatc ttattatctc
     2341 aatactgttc tttaatagct catacatatc cgttatgact ggatcaagtt ccatcatccc
     2401 tgcctctatc tttgagaagt caaggatatc attgataatt ccaagaagtg tgtgcccgga
     2461 gacattcaca ttgtcaacat actgctgctg cagttgagta agcggtgtat ccttcagaag
     2521 atctgtaaaa ccgatgattc cgtttaaagg tgtccttatc tcatggctca ttttggcaag
     2581 gaattcactt ttggcgttgc tcgccgcctc agcaagctct tttgcttttc ttaactcctg
     2641 ttcacgctct gcctgtatct ttctttcatt ctgtctgagt tcattaagat cgttccttat
     2701 caaagcaatt gattcagtca gatatgcaag aggattatct ttggaaacca cggcatttgg
     2761 aatgactccc gactcttccc actgcaactc accgaacaca ttgcttaatt cctcaagatg
     2821 ggcaggtgtt acaatcatcg gtttttcttc cctggcactt ttgagttcca tgggactgtt
     2881 atttattttt ttaaatgtct cttctactga attcgttatg atatatctga tattcagaaa
     2941 cggagaaaat gcagtgatca gtgctctgtt gaaattatcg ggattaatta taatgcgggc
     3001 tccaccgtta ttatctgtct cctgcattct ttttttcact tccttggcaa actgctgcct
     3061 gcagcggaaa gatcttattg ccgcaagttc agaaaaattg gtgattatgt atggaagttt
     3121 tctaagatga ttctctacca gcacctttgt tgcaagatca ctcgacttct gaacatcgtc
     3181 aatagtggtt aagtttccgt gcatagaaac ataaaaaaga actccgggga tgtaaccaac
     3241 ctgatattta taattattct gagggttttc ataaacccac tccggtttaa aaatgatatc
     3301 actaaaactt atagccatct gatcttcttt cggtttttca ttcttttgtt caagaatggt
     3361 tacagcggct ttaaccgctt catgataatt ttttgcggaa gcaactttaa atgaagtcgg
     3421 tccgaaaaat ctgagcccct gattgatgaa aggcctgaac caggatggct cctcaatgaa
     3481 aataatgccc gtcatcgttg cacggtgttc gttgaagtaa tacatctgct ttttcatgtt
     3541 cttaaaggaa agccgccccg tcaactctga aagattcctt atctgaacaa aaggtttttt
     3601 tacccccgca gcagcacaga attcctcaac gagcatattg aatttatcaa gatctacatt
     3661 ttttaaactt cctctgcttt gaacatacac aatgttttcg ccgatcttct gcaaagtagt
     3721 gtggtaatct ttattgattc ttacatcggc aaactcagga agcgtcacta tcagccgacc
     3781 agtcaccgga catttttcct gattgtaagt atcattctgt ttcatcatta tgtttgatct
     3841 taaaccattc tttaaaccgt ttcaaaccat ccgcaacata atactctttt cttatttttt
     3901 tgccaagagt gttgggatta tcaataagtg caaaatttgc gtttacgggt tggaaatcat
     3961 ttgtgacggc actcattttt ctgagtattc cgccggtcat ggtctctttt ggaggaagta
     4021 tatctttgat attattgcct tttgtgatct cagcaataca gagggctgcc cacattcctg
     4081 aaaatatgct ttcgttgtag ccctcaactc ctgagagctg tccagcaaca aaaacatttt
     4141 ccattgttct cacttttacg ccgttttcga gaactatagg ggcattgaaa aaagaattgc
     4201 ggtgaacaga tccgaacctt gtgaattcgg cattctttaa cccgggaagc gttctgaaaa
     4261 ctcttttctg ctccccctgt ttcatccgtg tctggcatcc gacaaggttg agaacacttt
     4321 tttcactgtt ttcggctctg agctgaacca cagcataagg tcttctccct ttccattcaa
     4381 aacctacagg cctgaacggt ccgaatgaaa gtgtcttgaa tcccctttcc gccatcactt
     4441 ctatcggcat gcacctttca aaaaattccg gtttttcaat tgaattccac ggaatctttt
     4501 cagcagtaat tagtgcttca acaaattgag catattcatc tttatcaaga ggaatgttta
     4561 gaaaatcggg atcgcctttt ccatatctgc ttccccagaa acactctgtg agatcaatgc
     4621 tgtcaagtgt cacaagagga gcaatggcat cataaaacga tccaccttct gccgaaattg
     4681 aattcatcat aattttatct gtcagaggtc ctgtcgcaat gatataaaaa tctccctgga
     4741 atcttgcttt caactcttca accgtagata tgttcaaacc tttgtgaaaa gttatgcacc
     4801 cgttttcatc tatcattttt gagatcaatt ttgaaaattc gactctgttg acagcaagac
     4861 ttgcccctgc cggaacactg cattctgatg ccgcttttaa aagaaacgat cccatttcac
     4921 gcatttcctc ttttaaaagg aaacttgccg ttgaaggaga ctccgattta aaggaattgg
     4981 aacagacaag ttcagctgga gacggttctg aatatacttc ttcatttaaa aaatgaggtt
     5041 tgcagtctat caattcaact gaaaaaccat tttgtgcaaa ctgtagggcc gcctcggatc
     5101 ccgcaagacc ggcaccgagg atgactattt tcattttggc ttgtgaccgc agttctggca
     5161 gactttgccc gatcttttct tttcaactat atatgcaaat ccgcattttt cgcacttctc
     5221 tcccgcaaca ggcggatacc agcttacgaa atcacatttc ggataattgg tacatccgta
     5281 gaatgccttc cctttttttg atcttttcgg agttattttt ccgtcacaac cctctttcgg
     5341 acatgctgca acgaactttt cctctctgat tatcgttttg cacttaggat aagcgctgca
     5401 tgccttgaat tcaccgaact tgccgttctt gacaaccagc ggactgccgc agttgggaca
     5461 tttttcatca agctttctat ctgctgactg ttcaaccgac ttcaagtgaa cttcttcacc
     5521 gaccttctct atcgaaccgg caaagtcaca atcaggaaaa gtttcacagc ttaagaacgg
     5581 ctgattccct tttttcgacc atctgacaac aagtttcttt ccgcatttcg gacacgcttc
     5641 ttcagcatat atcctctttt tgccctcttt tttcacctta cttatgcccg agtcgagaac
     5701 cttattgaaa gtgccgtaga atgactgaac aacatcaagc cagtccctgt cacccgtttc
     5761 aacggtatca agttcagatt ccattccggc agtaaaaccg atgttgatta tctcaggaaa
     5821 gaatgacaca agagtatcgc ttacaagaat tccaagctct gtagccatca aagcaccttt
     5881 aggatctttc atcctttcaa tgtagttgcg ctgaactatc tgctttatta cagttgcgta
     5941 tgtcgaaggt cttcctactc cctgttcttc aagctccttc acaagacttc cctcggtaaa
     6001 cctcggtgac ggcttcgtga aatgctgttc agcatcgact ttgttaagtt cggctgattc
     6061 accctgcaga acttccggag tcaggtcctc gcgaccattc tttttatata tctttctata
     6121 accttcaaac tgaattacgc tgcctgtttt tttgaaaagg aattttctgt ttgaatcttc
     6181 aatggtgata acagtagttt cgtaaaccgc atcctgcatc tgtgttgcaa caaaccttct
     6241 ccagatcagc gtgtagatgt tgagctcatc tttcgtgagt tttgatgcga ccttgtcggg
     6301 agtgagcgca acacttgtcg gtctgatggc ttcgtgtgca tcctgagttt ttcccttctt
     6361 gttagcaaaa actctcgtct tgggtgaaag atagttcttt cctatattgt ctgaaatgta
     6421 atttctcaga gcggcatttg cctcttccga tatcctgaaa gagtcagttc tcatataggt
     6481 tataagaccg gtaacccctt cttcaccaag atccttacct tcataaagtg actgtgctat
     6541 tctcattatc tgttcagcag tatagttgta aagacggctc gcctcctgct gaagattgct
     6601 tgtgttaagc ggtgggagcg gagattcctt tttctctttc ttttcaaccg aagtgactga
     6661 aaaagcacct gccgatatga tctctttctt tatttcttcc gctttcaatc cgtccgccac
     6721 actgaccttt tcacctttta ttttatcaag cagggcatcc acttttcctg ccttcacttt
     6781 gaaatcggct ttgaaattcc agtattcttc cggaacgaat gcctttatct ccttttctct
     6841 ttcagcgacc atctgcaatg ctacagactg gactcttccg gcactcagcc ctttctttat
     6901 gcttctccac aggaacggtg aaagtttgta tccgactatt ctgtcgagta ttcttcttgc
     6961 ctgctgagag ttgtagagat tgtcattcaa ttttgaaaag acatcgatcg ctttaagcac
     7021 gcctgtcttg gttatctcgt ggaaaagaac tctgcgcact tcccttcctt caaaaaggga
     7081 tgctatatga aaagcgatcg cttcaccttc acggtcaggg tcggagccga tatatatctc
     7141 ttcagcttcg gatgcctcct ttttaagcat tgctataacc ttttccttac ccgggatcac
     7201 ttcgtatctc ggcttgaaat tgttttcaat atcaacaccc aaccgctttg aaggaaggtc
     7261 gataatgtgt cccattgaag caacgacctt gaacccttta aggtattttg tcagagtctt
     7321 tgcctttgtc ggtgattcga cgataagaag tttcatagct ttacctcgct gtatctgtaa
     7381 atttttccgg gaagttcatc gatcatctcc ttcatcataa gaaaaagcag tattgaagga
     7441 agttccgtag ctgaaaatcc gctgcgttcc gaaagtgtgt caaaatcaat cccctcaccg
     7501 ctttcaacga tcagacggta aacctttttt tcatcatctt caagatctgt atccgtcaga
     7561 acattcaacg gcttaatatg ttcactaaaa aaatttttgc aattaaaaaa ttcatcaagc
     7621 tgttcaagat catacaaaag ttcgacaccc ggatcttttt tccggctgtt gagaaactga
     7681 taatttcccg taaaaagttc atcatctatc ggtcccggaa ttacaaaaac ctttttatca
     7741 aattcccttg caagccgcgc cgttataaga cttccgcttt ttgttgacgc ctgaacaagc
     7801 acaagcacgg aacacatttt tgcaacgatc tcgttcctta tcgggaaatt cttttttaac
     7861 ggaggagtct gaggcgggaa ctgggaaatt agtgcgcaat tctgccggat ctcttctgca
     7921 agtccgatat tttcttcagg atatatcaca tctattcctg ttcctaaaac cgctattgtc
     7981 cttccgccgt atttgagagc agatctgtgt gattctgtgt ctatccccct tgcaagaccg
     8041 cttatgactg tttttgagtt catgacagct ttttgtgcca ccgcctgagc catcttgatt
     8101 ccgtaaagtg acggttttct gcttccgacg atcgcaacac ctcccgcatc ttcagggatc
     8161 aaagaccctt cgatataaag cggatacgct atttccggtc tgcattttaa aattttatca
     8221 ggatgtctgt tgtcaccggc gtcaattttc atcattttaa agattccaga aaatttttca
     8281 tttcaagtat ctggtcatca ggagttgagg cacctgcggt aagtcctgca acagaacatt
     8341 ttttaagttc attcagcatc tcttcggtga aatcgtcaac agattcaacc atgtaagatt
     8401 tctcgttctt ttctttaact acctgaaaaa gcttcatcgt gttggatgag ttcatccctc
     8461 ccagaactat catcgcatca acccttgtcg caagttctct tgccgaatcc tgacggctga
     8521 atgtcgcatt gcatatcgtc tgagccgatt caaatatgtt ctcttttttc tgctcaatga
     8581 attttcttat ctttctaagc atattgtcat agttggcagg ccttgatgtg gtctgagcga
     8641 cacagtatat tttcctgtgt tccgttataa attcttccac ttcactccac ttttgcgaaa
     8701 cttctgcggg agatacgaca agaaaatggc cttttatcct tgaagttatc ccctgaactt
     8761 ctgaatggtc cttatccccc atgacaataa cggcatatcc gcctgaagat ttcttttcaa
     8821 cgagctgatg gatcctttta acataggggc aagtagtgtc aacaaccgta agtccttgga
     8881 ttttttcaag tttattttca gcatcaagtg tgatcccgtg gcttctcaca acaacgatac
     8941 aactttcagg aatgttttcc gtgtctctta cttcgactcc gatcgaagca tagtgctcaa
     9001 caactctcgg attatggatg agcgggccga gcatcactgc ctttcttttt tccagttctg
     9061 ctgtttttac cgattcttca aggagatcaa gcgctcgtct tactccgaag catactcctg
     9121 aattttcagc aacaatgact ttcaatttaa tttttccctt tcacgagcat attttacgat
     9181 aagttcagcg gtttcaaaaa gatcaatgtc agaattatca accagaactc cgtcaacagc
     9241 gatcttcagc ggcgctattg ccctttttga atcctgatca tctcttcttt ttatctcttc
     9301 caccagattt tccatagtta tttcaacacc ttttgctttc agctcaagca ttctccttgc
     9361 cgcccttttt tcaggggatg ccgtaagaaa tatcttgagt tcggcatctg gaattatcac
     9421 tgttccgatg tcgcgaccct ccattacaac tccgccggct cttgcatatc ttttctgcat
     9481 ttcaagaagt gcttctctta caaaagaaag tgccgaaaca gagcttgcaa gcatactcac
     9541 tttttcagtc ctgatgaaag gagtaatatc ttcaccgtta aggaaaatgt ggttcacatc
     9601 atcttccata ctgaatgaaa catcaaagtt atcaagcagt tgtcttattc cggcatgatc
     9661 ttcaagtttt actgtttcat tcattgcata ggcaagagag cggtatatcg caccagtatc
     9721 gacatactga taaccgagta accctgcaac cattctgctc acactgcttt tgcctgctcc
     9781 ggcaggtccg tcaatagcta ttctgatctt tttcatggat taaacacctc ctgaaaagca
     9841 ggccgattat agcaaccgga tcaataaaat caagggggcg tatcaagcca tctaaaatct
     9901 taccgaagac aaatatgtca cgccatgtaa a
//
LOCUS       DAOOYB010000101         9924 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_7400_length_9924_cov_8.588712, whole genome shotgun sequence.
ACCESSION   DAOOYB010000101 DAOOYB010000000
VERSION     DAOOYB010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9924
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7400_length_9924_cov_8.588712"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>227)
                     /locus_tag="PL056_07325"
     CDS             complement(<1..>227)
                     /locus_tag="PL056_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46864.1"
                     /translation="GDSNADGNSVPASMDWGGVWLNSAMVGISIKNTEISYGGESDEG
                     ADSGLRVDGGTSLLIENVEFRNNEDDISIFG"
     assembly_gap    228..327
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<328..3599)
                     /locus_tag="PL056_07330"
     CDS             complement(<328..3599)
                     /locus_tag="PL056_07330"
                     /inference="COORDINATES: protein motif:HMM:NF014869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HPM46865.1"
                     /translation="MRFLSTVLIFVFAGMIVSCEIAEYSLEDNLSEEISNQNRVQFNE
                     SDLITSSIKVFKGIASEGLVFDWNEKTQSPLYVKFGNDGFSSNEFYGMEKEELALYFM
                     ENVSDAFRLQPEDELKFFKKQGNEGGFTTIKLTQTYKGIPVDGAMINVGLGPKNQVRV
                     LIGALVPDLALDVNPFISDYQAIEAVKESEQFEILDATAELVIYSRALVGEESSGKEA
                     NVLAWKIVTTTKNPLNDKVYYLDAQSLEKIKEIAVAVSISREVFLQGTDMVSSPYGAL
                     IMDETNGHDTTDQDAIKSFDLAQMYNNFLVNNFFPNGFSSVGNLPYRVIIHSSWAASS
                     GGAYFQNGYKYAAFSSGMVALDTFYHEWTHGFLFNTANLEYNLIQSQALHEAIADMIA
                     SFNDVNWTIGESTDSGQIVRRIDYPNDGLLLWGYHDHMDQYDDEDKYKESTIFSQAGY
                     LYGETEGSVHYHRLDTSKMNPLFGIGRNNAGRITIYGAGNYLFPTANFKAGRWAILQG
                     CNDLVEQSGSGINSSYCTRINSAFNAVGITKYSAPTVTVYPVAGDVSGCIDQSGTGFP
                     NKADITLIFKDEVAGFESRTNVVSDSDGNVVWYFGLECEGIVEETEAIKAKQRAESFL
                     NRVDPNDVGFTGRQMLYRAEYWDGGLVNSNSVAYTLTYTGECTPVCDGKSCGPDGCGG
                     YCGTCYGTKYCNTGVCTDPGAPVTTKCGNIDGNETWNLAMSPVKITCDVTVNGSLTIM
                     PGVIVEFQDPDDDLYINMGANFTANGDKDNPIIFTSDGEMAVGSWGGIYFNGDFSSGQ
                     ISYAEFRHGGKFTNSRAGGFPIKVNKKSPTISNIKLISNRRNAVGLELGNYSSNIRLS
                     VVGVPYYIEANYLLNQEDLIIGAGSTLTIDPGVIVKLHSDEVYSGHGADIYINGRIVA
                     NGTAEMPIYFTSFRNDMIGGDSNADGNSVPASMDWGGIYLNKDALGLPASSLKNVVIN
                     YAGETDEGLRYPLKVSGYTQPTIESVTISSSRRNAIALDMGTYNSDFRLNIVGIPYYI
                     ESVYSWSQQDLTVAAGATMTVDPGVIIKFQDDLTYGNAADLIVNGRIVANGTAEMPIY
                     FTS"
     gene            complement(3612..4457)
                     /locus_tag="PL056_07335"
     CDS             complement(3612..4457)
                     /locus_tag="PL056_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46866.1"
                     /translation="MFRVMIVSIFGLFFISCGISVGFAQTHGGTSLSYQVPVKDMKFE
                     GEVSSTVKGPSVFCIFPAYKPLMKQAMEKLNAQAGLKNGNKAVFNVRADVEDKFYLFW
                     CTRHVTVSGDVYRIVDDKELEKEKIMQATRQELAKQDQIRQEKKNEEQNELQKKRDEE
                     EKKRLSIEKIPDSELKKPEERQKFLSKYWEALKNKNLMMIKNEVVYSLMSKPGYVKQE
                     GFYFLGDAALIEKDIAAAENHYRKGLSMEGADQEALKFHLQQLGLMMPELKESIQAIY
                     NKVVK"
     gene            4620..4880
                     /locus_tag="PL056_07340"
     CDS             4620..4880
                     /locus_tag="PL056_07340"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPM46867.1"
                     /translation="MSIRENFGQRLKVLRQHRKMSQDDVSLASGIDRSYISQIETGKS
                     SPTLDIIDKIAKALNVSTDELFIFGVSEPERTYDGEPFENAD"
     gene            complement(4904..5932)
                     /locus_tag="PL056_07345"
     CDS             complement(4904..5932)
                     /locus_tag="PL056_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46868.1"
                     /translation="MRRSFLFLLVLAGLLLFSSCGDDTKSKCLPKGATRCVGNILQIC
                     ADGSWLNLEDCNENGNICAENNDDETDSAACVKPDIGNTGNSGDSGNTGNTGNTGNTG
                     NSGDTGDTGNSGNSGNSGDTGNTGDTGDTGNSGNSGNSGNSGNTGDTGDTGDTGDTGN
                     TAEDTYPEETETSKKAGDIARNITFFDELDTEHRLEEWYAKKNPESKLIWLIFAAYDC
                     MYSSKLRNDLYLIADKDMIDDGLNVILIMNGLLSGPAPDQEPAKLFKMKDAMAAFNLE
                     NSYYTYGYLKYDQQNNFSRYVNQGYPVSVFIDAKDMKIVKHIEGWTSEEDSINEIKSF
                     VSDSLSGK"
     gene            complement(5959..6732)
                     /locus_tag="PL056_07350"
     CDS             complement(5959..6732)
                     /locus_tag="PL056_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46869.1"
                     /translation="MPRIDTLSGNFKICQPDVGQRYSTDDMLTAWMTIICSEEDPPSR
                     FLDLGSGLCSVPMIVLWKFSQISGVGIELRENRRELGLKSLEMNGLSNRFKLLHGDLR
                     ELELDEKFSLITSTPPYYTDAEGPISPHDDKSAARFELNGNIEDYFETADRHLLENGM
                     FITVYPYQYRDRVYTAASKFKMFINKRVDMVPRSGKQPLISMYSISYNDNAETVSTIT
                     VRDKAGKYTDEYDQARIICGFQSVDKEKIVDKIKSFVSI"
     gene            complement(6742..8424)
                     /locus_tag="PL056_07355"
     CDS             complement(6742..8424)
                     /locus_tag="PL056_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HPM46870.1"
                     /translation="MKKFILVVMSVVLIVFFSGCSKKKKTQDDNDITAPDSDIESVET
                     DESMLDEDVNYPDSVEPDLEEQDIEEQDGAVEPDEIETDDDPEDYDEDTFDRFANVIC
                     VAVTGNDNTGDGTVAAPFATIGKAIAVAVDGKEIYVSAGEYNEALTINKDNIKIIGGF
                     NEDFADGGLYSSDMIGAGEENITKIISTEESVVTFDGTLDNFLFEGFVVESTRDTGHA
                     SSAITVKSGSPEIAQNTLIGGNPEGSLMGALSGSLSAGLVIGGGSPVIHNNRIIAGEA
                     VDNFTTIGTPTAASIGIVIGEISSIGAESSVNTEIYNNIIIAGHSKDEGTDGGNVGIG
                     VYRNNGDVKIHGNIISAGENKYKARSYGVLLSKNTGIVLYNNIIHGGKSTDLTSGIYF
                     YDSTTAKVFNNTLDCGESTMTTTAVKFSGKYSSPELRNNLLFCVNDDNPVYEFNTNQI
                     TPFAPVFENNVIVGGSTGVTIMGTQNSSDSLYKSATNIYLPSKTVADIGFVNYVDGSL
                     YYANDLHLTADSKDIDGNDITTAGSDLSEIFIKDKDGKTRTVPWSAGAYELD"
     gene            complement(8493..9533)
                     /gene="queA"
                     /locus_tag="PL056_07360"
     CDS             complement(8493..9533)
                     /gene="queA"
                     /locus_tag="PL056_07360"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013298040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HPM46871.1"
                     /translation="MDTKLFNYHLPEELIADNPLKRGESRMMVLDRSNPVIKHSKFGN
                     ITDFIDENTTVVFNSTKVIPARIFGKRATGGVVEFLLIKQIGSGLWQVMVKCSKHLGR
                     GEKVNFENGVTAEIMSRNEQFADVKFSLNDTQLLKFLDEFGDVPLPPYILKRRNEKKS
                     RFEDREKYQTVYAQVPGSVAAPTAGLHFNEEILKKIHEKTRGHVENIVLDVGLGTFLP
                     VKSEKIEEHKMHKERFVITKDCASRLNDDKKAGRKILAIGTTTVRALESADDEKGIIH
                     PCDKETGIFIYPGYKFKFVDIILTNFHLPQSTLLMMISAFADREFILNAYEEAVKEKY
                     RFYSYGDCMLII"
     gene            complement(9533..>9924)
                     /locus_tag="PL056_07365"
     CDS             complement(9533..>9924)
                     /locus_tag="PL056_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46872.1"
                     /translation="KSVKCPYCKKSSLYRWKRSVSSKVLSDKLGVGKVKSIQVVRSET
                     RVESLIVDNKKIPVNKFRELVGFSVIWSNDFVVRKEGSNFIFEGKGGGHGVGVCQWGM
                     AGMAEKGKNHFEIINFYLTGVEIRKMY"
BASE COUNT         2812 a         2198 c         1601 g         3213 t
ORIGIN      
        1 gccaaaaatt gaaatatcat cctcattatt acggaattca acattttcaa taagaagaga
       61 agtaccacca tctactctca accctgaatc agctccctcg tcactttcgc caccataaga
      121 tatttctgta tttttaatag aaataccaac cattgccgag tttaaccata cacctcccca
      181 gtccatagaa gctggaacgg aattgccgtc tgcgtttgaa tctcctcnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnngat gtaaaatata tgggcatttc tgctgtgccg
      361 ttggcaacaa tgcgaccgtt aacgatcaaa tctgcggcgt taccgtatgt taggtcgtcc
      421 tgaaatttta ttatgactcc cggatcaaca gtcattgtag ccccggctgc aacggtgaga
      481 tcttgctgac tccaactata tactgattct atgtaataag ggatacctac tatattcaag
      541 cgaaaatctg aattatatgt gcccatatca agagcaatcg catttcttct gctactggat
      601 attgtaactg attctattgt tggctgtgta tagccactta cttttaatgg atatcttagt
      661 ccttcgtcag tctctcccgc ataatttatt acaacatttt taagtgatga ggctggcaat
      721 cctaaggcat ctttgttcaa atatattcct ccccagtcca tagaagctgg aacggaattg
      781 ccgtctgcgt ttgaatctcc tccaatcata tcattcctga aagatgtaaa atatatgggc
      841 atttctgctg tgccgttggc aacaatgcga ccgttaatat aaatatctgc accatgtccc
      901 gaatatacct catcgctatg aagctttacg ataactcctg ggtcaatagt caatgtgctt
      961 ccagcaccaa tgatgagatc ttcttgattt aaaagatagt tagcttcaat gtaataagga
     1021 acaccgacaa cacttaacct aatattgctg gaataattac caagctctaa accgacagca
     1081 ttgcgtctat tagaaattaa ttttatgttt gatattgttg gagacttttt attaactttt
     1141 atcgggaaac cacctgcgcg actattagtg aatttccctc catgtctaaa ttctgcatag
     1201 ctgatttgcc cggaactaaa gtctccattg aaatatattc caccccatga cccgactgcc
     1261 atttcaccgt cagaagtaaa tattattggg ttatccttgt caccattagc agtaaaattt
     1321 gcacccatat taatatacaa atcatcatca ggatcttgaa attctacaat tactcccggc
     1381 ataattgtta atgaaccgtt aacagtcaca tcacatgtaa ttttcactgg actcattgcc
     1441 agattccaag tctcatttcc atctatattt ccgcacttgg tagtaacggg agcacccgga
     1501 tcagtacaga cacctgtatt gcaatatttt gtgccataac aagtcccgca ataccctccg
     1561 catccatcag gaccacatga ttttccatca cagacaggag tgcattctcc cgtataagta
     1621 agggtgtagg caactgaatt tgaatttact aatccaccat cccaatattc ggcacggtat
     1681 aacatttgcc tgccagtgaa accgacatca tttggatcga ccctgttcaa aaaagattca
     1741 gcccgttgtt ttgcttttat tgcttctgtt tcctcaacaa taccttcaca ctcaagacca
     1801 aaataccaaa caacatttcc atctgaatca gaaacaacat tggttctact ttcaaatcca
     1861 gcaacttcat ctttaaaaat aagtgttatg tcagctttgt tgggaaatcc tgtgccgctt
     1921 tgatctatgc atccggaaac atctcccgct acggggtaaa ctgtaactgt cggcgcagaa
     1981 tattttgtta ttcctaccgc attaaatgca gaatttattc ttgtacaata agaagaattt
     2041 attcctgaac cagattgctc tactaaatca ttgcaacctt gtaatattgc ccaccttcca
     2101 gctttaaaat ttgctgttgg aaacaaataa ttaccagcac catatattgt tattcttcca
     2161 gcgttgtttc ttcctatacc gaataaagga ttcattttag atgtgtcgag tctatggtag
     2221 tgaacactgc cttctgtttc gccataaaga taccctgctt gactaaatat tgtagattcc
     2281 ttatatttat cttcatcatc atattgatcc atgtgatcat gataacccca caatagaaga
     2341 ccatcattcg gatagtctat acgccttact atttggcctg agtctgtact ttctccgata
     2401 gtccagttaa catcattaaa tgatgcaatc atatctgcaa ttgcttcatg caatgcttgg
     2461 gattgaatta aattgtattc taagttggca gtattaaaaa ggaatccgtg tgtccattca
     2521 tgataaaaag tatcaagcgc aaccattcca cttgaaaaag cagcatactt gtaaccattc
     2581 tgaaaataag ctccacctga acttgcggcc caagagctat gaataataac ccgataagga
     2641 aggttgccaa cgcttgaaaa tccattcgga aaaaaattat taacaagaaa attattatac
     2701 atctgtgcca aatcgaaaga ttttatcgca tcttgatccg tggtgtcgtg tccattagtt
     2761 tcatccataa tcaaagcacc atatggagat gaaaccatgt ctgttccctg caaaaatact
     2821 tctcgtgata ttgatacagc tacagctatt tctttgattt tctcaagaga ttgagcatcc
     2881 aaataataaa ccttatcatt taaaggattt ttagtagttg ttacaatctt ccaggcaaga
     2941 acattcgcct ctttgccaga tgattcttct ccgacaagtg cccgactata aataaccaat
     3001 tctgctgttg catcaagtat ttcaaattgc tcactttctt ttaccgcttc aatcgcctga
     3061 taatctgaga taaacggatt gacatcgaga gcaagatcag gaaccagagc accaataaga
     3121 actcttacct gattttttgg tccgagccca acattaatca tagctccgtc aacagggata
     3181 cctttatatg tctgggtgag tttgattgtg gtgaatccgc cttcgttacc ctgttttttg
     3241 aaaaacttca attcgtcttc cggttgcaac ctgaaagcat cactcacatt ttccatgaaa
     3301 taaagtgcca gctcttcttt ttccattcca taaaactcat tagaggaaaa tccatcattc
     3361 ccaaatttta cataaagtgg agattgagtt ttttcattcc agtcaaacac caacccttct
     3421 gaagcgatcc ctttaaaaac tttaattgaa gatgtgatca gatctgattc attgaattga
     3481 acacggttct gattagaaat ttcttctgat aaattatctt ccaaagaata ctcagcaatt
     3541 tcacaggaaa caatcatccc tgcaaaaaca aaaatcaaaa cagttgataa aaatctcata
     3601 gggatctcca tttatttaac aactttatta taaatagcct gaatgctttc tttcagttca
     3661 ggcatcatta agcctaactg ctggagatga aactttaatg cctcctgatc tgctccttcc
     3721 attgaaagtc ctttcctata gtggttttcg gctgctgcaa tgtctttttc gatcagtgct
     3781 gcatcaccga gaaaataaaa tccttcctgc tttacataac ccggcttgct catcagtgag
     3841 taaacgactt cattttttat catcatcagg tttttgtttt taagtgcctc ccagtattta
     3901 gaaagaaatt tctgtctctc ttccggtttt ttcagttcag aatcgggtat tttttctatg
     3961 gaaagtcgtt ttttttcttc ttcatctctt tttttctgca actcattctg ctcttcattc
     4021 ttcttttcct gtctaatctg gtcctgtttt gctaactcct gtcttgttgc ctgcataatc
     4081 ttttcctttt ctagctcttt atcgtcaact attctgtaaa catctcctga cactgtcaca
     4141 tgtctggtgc accagaatag ataaaatttg tcttccacat ctgctctgac attgaaaaca
     4201 gctttgttgc cgttttttaa gccggcttgt gcattaagtt tttccattgc ctgtttcata
     4261 agtggtttgt atgccggaaa aatgcaaaac acgcttggac cttttaccgt cgaagaaact
     4321 tctccttcaa atttcatgtc tttgactgga acttgataac ttaagcttgt tccaccatgc
     4381 gtttgtgcaa aacccacact gattccgcaa gaaataaaaa acaacccgaa aattgaaact
     4441 atcatgactc tgaacatttc aaaaatctcc tcaaagataa ataattagct taaaaacaca
     4501 tattgtatga attttatagt tttttattat agtgtcaact ttttgcaaca tttcaaataa
     4561 accgcttttg ttgtctatat cctacagctt ttctatctgt tttccatatt atttccgcca
     4621 tgagtattcg agagaatttc ggtcagcgtt taaaggtgct tagacagcac agaaagatga
     4681 gtcaggatga tgtgtccctt gcttcgggca ttgacagaag ctatatttca cagatcgaga
     4741 cgggcaaatc ttcccctact cttgacatca tagataagat cgcgaaagca ctgaatgttt
     4801 cgactgatga gctttttatt ttcggtgtgt cggagccgga aagaacttat gacggcgaac
     4861 cttttgaaaa tgctgattaa aagacctttt ttctttgaaa attttatttt cctgacaagg
     4921 aatctgaaac aaaagacttg atttcattta tagaatcttc ttcactggtc cagccttcaa
     4981 tatgttttac tattttcata tcttttgcat caatgaaaac actcaccgga tatccttgat
     5041 ttacatatct ggaaaaattg ttctgctggt catacttaag ataaccgtat gtgtaatatg
     5101 agttttcaag gttgaatgcc gccattgcgt ctttcatttt aaacagtttt gccggctcct
     5161 gatcaggtgc aggccctgac agcagaccgt tcattatcag aataacattt aatccgtcat
     5221 cgatcatatc tttgtcagca atcaaataca ggtcgttcct taattttgaa ctgtacatac
     5281 aatcatatgc cgcaaatatc agccatataa gttttgattc cggatttttt ttggcatacc
     5341 actcctctaa acggtgctct gtgtcgagct catcaaaaaa agtaatgtta cgggcaatat
     5401 ctccggcttt cttagatgtt tctgtttctt caggatatgt atcttctgcg gtgttgcctg
     5461 tatctccagt gtctccagtg tctccagtgt ctccagtatt acctgaatta cctgaatttc
     5521 ctgagttccc tgagtttcca gtatcaccag tgtctccagt atttcctgta tcacctgaat
     5581 ttcctgagtt ccctgagttt ccagtatcac ctgtgtcacc actatttcct gtgtttcctg
     5641 tgtttcctgt gttgccagtg tttccagaat cacctgaatt accggtattg ccaatatccg
     5701 gtttaacaca ggcagcggaa tcagtttcat catcattgtt ttctgcacaa atatttccat
     5761 tctcattgca atcttccaag ttcaaccacg aaccatcggc acaaatttga agaatattgc
     5821 cgacacaacg ggttgcgcct ttcggcaggc attttgattt tgtatcatct ccacagctag
     5881 aaaaaagaag caaaccggcg agaactaata aaaataaaaa tgatcttctc atgatacctc
     5941 tttgaattat cagcatttct aaatagatac gaacgatttt attttatcaa cgattttttc
     6001 tttgtcaacc gactgaaatc cgcagataat tcttgcctga tcatattcat cagtatattt
     6061 tcctgcttta tctctgactg taatggttga aacagtttca gcattatcat tataacttat
     6121 tgaatacatt gagataagag gttgctttcc tgatcttggg accatgtcaa cccgcttatt
     6181 tatgaacatt ttgaattttg aagcggctgt ataaactcta tccctgtact gataagggta
     6241 aactgttata aacattccgt tttccaacag atgtctgtct gccgtttcaa aataatcttc
     6301 gatgtttcca ttcagttcaa atcttgccgc ggacttgtca tcgtgcggac ttattggacc
     6361 ttcagcatct gtgtaataag gaggagttga agtgatgaga gaaaatttct catcaagttc
     6421 aagttctcga agatctccat gcaacagctt aaaacgattt gacaatccat tcatttcaag
     6481 tgatttcaat ccgagttctc ttctattttc acgaagctct atccctactc ccgatatttg
     6541 agaaaacttc cacagaacta tcataggaac agagcaaagt ccactcccaa gatcaagaaa
     6601 acggctcggc ggatcctctt cactgcaaat aatcgtcatc cacgcagtaa gcatatcatc
     6661 agttgaatat ctctgcccga catcaggctg acagatcttg aaatttcctg aaagagtgtc
     6721 tatacggggc ataaggataa attaatccag ttcgtaggca ccggcagacc acggaacagt
     6781 tcttgttttc ccatctttat ctttgataaa tatttcagaa agatctgagc cggcagttgt
     6841 gatgtcattt ccgtcaatat ccttgctgtc cgctgtcaaa tgaagatcat ttgcataata
     6901 aagagaaccg tcaacataat ttacgaaacc gatgtcagca acagtttttg aaggaagata
     6961 tatgtttgta gctgacttat acaatgaatc acttgaattt tgtgtcccca ttattgtaac
     7021 accggtactt ccgcccacga tgacattgtt ttcaaaaacc ggagcgaacg gggttatctg
     7081 gttagtgttg aattcataaa cagggttgtc gtcattaaca cagaaaagga gattgttcct
     7141 caattcaggt gaactgtatt ttccggaaaa cttcacggca gtcgtagtca ttgtgctttc
     7201 accgcaatcg agcgtgttgt taaacacttt tgctgttgtg gaatcataaa aatatatgcc
     7261 tgaggtcaga tcggttgact tgccgccatg aattatgttg ttataaagaa caatgccggt
     7321 attcttactc agaagaactc cgtaacttct tgccttatat ttgttctccc ccgcactgat
     7381 gatatttccg tgaattttca catcaccgtt atttctatat actccgatcc cgacatttcc
     7441 accgtcagtg ccctcatcct tactgtgacc tgcaattata atattgttat aaatttcagt
     7501 attaacagat gattctgcac ctattgaact tatttctcct atgacaatac ctattgaagc
     7561 cgcagtaggt gttccaatcg ttgtgaaatt atcaaccgct tcacctgcaa ttattctgtt
     7621 gttatgaatt accgggctgc caccgccaat cacaagacct gcagaaagag aaccgctcaa
     7681 agcccccatt aaagaacctt cggggtttcc accgatcaaa gtgttttgcg ctatctcagg
     7741 actgcctgat ttgactgtaa tagccgaaga agcatgccct gtatcacgag tgctttcaac
     7801 aacaaagcct tcaaacagaa aattgtcgag agtgccatca aaagtgacaa ctgattcctc
     7861 tgttgaaata atttttgtta tattttcttc acccgcacca atcatatcag aactgtaaag
     7921 accgccgtct gcaaaatctt cgttgaagcc gcctatgatc tttatattgt ctttatttat
     7981 tgtaagcgct tcattatatt cccctgcaga aacataaatc tcctttccat caactgcaac
     8041 tgcaatagct ttgccgattg ttgcaaaagg agcagccaca gtaccatctc cggtattgtc
     8101 gttcccggtc acagcaacac agatgacatt cgcaaaacga tcgaatgtgt cttcatcata
     8161 atcttcagga tcatcatctg tttcgatctc atccggctct actgcaccat cctgctcttc
     8221 aatatcctgc tcttccagat cgggttcgac actatcagga tagttgacat cttcatcaag
     8281 cattgattca tctgtctcaa cagattcaat gtcactgtca ggagctgtta tatcgttatc
     8341 atcctgcgtt tttttctttt ttgagcatcc tgaaaagaac actatcaaaa caacactcat
     8401 tacaacaaga ataaactttt tcatgacatc ctcctaagat gaatagaaat tacggagatt
     8461 ataatgtttt taattgagaa ttcaaataaa attcaaataa tcagcataca atcaccataa
     8521 gaataaaaac ggtatttttc ttttaccgct tcttcatagg cattaagtat gaactctcta
     8581 tctgcaaatg ccgatatcat cataagcagt gtgctttgtg gaaggtgaaa attagtaaga
     8641 attatgtcaa caaatttaaa tttatatccc ggataaatga aaatccctgt ctctttgtcg
     8701 catggatgga tgataccttt ttcatcgtca gcactttcaa gtgctctgac cgttgtagtt
     8761 cctattgcaa ggatctttct tccagctttt ttgtcatcat taagccgtga agcacaatct
     8821 ttcgtaatta cgaacctttc cttatgcatt ttgtgctctt cgatcttttc acttttaaca
     8881 gggagaaatg tcccaagccc gacatcaaga acaatatttt caacatgacc tctagtcttt
     8941 tcatggatct tttttaatat ttcttcattg aaatgaagtc ctgcggtcgg tgcggcaact
     9001 gatcccggaa cttgtgcata aactgtctga tacttttctc tgtcttcaaa tcggcttttt
     9061 ttctcgtttc ttcttttcag aatatacggc ggcaaaggaa catcgccgaa ttcatcaaga
     9121 aatttaagaa gttgagtatc attcaaagag aacttcacat ctgcgaactg ctcatttctt
     9181 gacattattt ctgccgtcac accattttcg aaattgactt tttcgcctct cccgagatgc
     9241 tttgaacact taaccatcac ctgccacaat ccgcttccga tctgctttat cagaaggaac
     9301 tcaacaactc cgcctgttgc cctttttcca aaaatgcggg caggaataac ttttgttgaa
     9361 ttaaagacca cagtcgtatt ttcatcaata aaatcggtta tattgccaaa ttttgaatgt
     9421 tttatgaccg gattactcct gtcaagcacc atcattctgc tttcacccct tttcaaagga
     9481 ttgtcagcga tcagctcttc aggaagatga taattgaaaa gttttgtatc catcagtaca
     9541 ttttcctgat ctctacaccg gtaagataga agtttataat ttcaaaatga tttttccctt
     9601 tctccgccat cccagccatg ccccattggc agacgccgac accgtggcct ccaccttttc
     9661 cttcaaaaat aaaattagac ccttcttttc tgacaacaaa atcatttgac cagataacag
     9721 aaaaaccgac taactctcta aatttattaa cagggatctt tttgttgtca actatcagac
     9781 tttcgactct tgtttcgctt ctaaccacct gaatagattt aactttacca actccgagtt
     9841 tatctgaaag cacttttgaa ctcaccgatc ttttccaacg gtaaagagat gattttttgc
     9901 aataaggaca ttttacagat ttaa
//
LOCUS       DAOOYB010000102         9856 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_7467_length_9856_cov_10.703398, whole genome shotgun sequence.
ACCESSION   DAOOYB010000102 DAOOYB010000000
VERSION     DAOOYB010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9856
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7467_length_9856_cov_10.703398"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            31..399
                     /gene="rpsM"
                     /locus_tag="PL056_07370"
     CDS             31..399
                     /gene="rpsM"
                     /locus_tag="PL056_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HPM46873.1"
                     /translation="MVRIAGVDLPRKKRMEIALTYIYGIGRTSAQKILDEAGIDRSTK
                     SDDVDDSMAIKIRAVIDQKFKVEGDLRKDVIMNIKRLMDLGSYRGRRHRRGLPTRGQR
                     TRTNARTRKGPRRNLMKKKK"
     gene            414..794
                     /gene="rpsK"
                     /locus_tag="PL056_07375"
     CDS             414..794
                     /gene="rpsK"
                     /locus_tag="PL056_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015752467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HPM46874.1"
                     /translation="MAPEKKKKVKKSIQTGLAYIQCSFNNTIVTITDVSGNVLSWSSA
                     GLKGFRGSKKSTPYAAQVAAEDAGKKAQDYGLRTLSVFVKGPGSGREAAIRGLSNVGY
                     NVTLLRDITPIPHNGCRPKKKRRV"
     gene            839..1468
                     /gene="rpsD"
                     /locus_tag="PL056_07380"
     CDS             839..1468
                     /gene="rpsD"
                     /locus_tag="PL056_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HPM46875.1"
                     /translation="MAKYKEAKCKICRREGAKLFLKGERCHTEKCSFERKPYGPGNHG
                     KDKKRVTEYKIQLREKQKAKAIYGMLEKQFSIFYDRSVLKKGATGENLLRMLECRLDN
                     IVYRLGFANSRAQARQLINHRHFSVNGRLTDIPSFIVKAGDVVVLKEKSRNSKIFKET
                     IESAGERLPVPSWLELNKGEFKGTVVRLPGREDITEDVKENLIVELYSK"
     gene            1483..2502
                     /locus_tag="PL056_07385"
     CDS             1483..2502
                     /locus_tag="PL056_07385"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF003513.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HPM46876.1"
                     /translation="MYRANWKTLIMPKDVRVVEQGADGAYARFVCEPLMKGYGITLGN
                     SLRRILISSLRGAAVIGVKIKGYEHEFATIPGIKEDVVDIILNIKQIRFVVHEDKLHT
                     LLLRKNVKGTVTAADIAETAGIKILNKDLVLFEMDEGVDFEMELIVGIGRGYVPSEAH
                     NQDIFSEGYIAVDSFFSPVVRANYKVTNARVKNSFDFDKMVFDLETDGSLSPRDALAY
                     AAMILREHTKFFISFSIDEKDELNASDSHEEVNMNLYRTIDELDLSVRSYNCLKNANI
                     RFIGELVKRSEAEMLKTKNFGRKSLNEIKILLIKMGLHFGMDIDNFPDQKIIEQIEKK
                     SGSKI"
     gene            2499..3062
                     /gene="rplQ"
                     /locus_tag="PL056_07390"
     CDS             2499..3062
                     /gene="rplQ"
                     /locus_tag="PL056_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014324054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HPM46877.1"
                     /translation="MRHRKAGRKLNRDSSHREAMLCNMLNSLVKNERIQTTFGRAKEL
                     QKIADKAVTLAKKNTTASISQLTKIVKSDREEVITKLLKEIGPRFASRAGGYTRIIKV
                     GFRRGDAAPTVIIEYLGADEPVQKKTKKKKTVKKEAPVVEAAPVVEAVPVVEAVPVVE
                     TPAVEEAPVPVEEVPVAEEVKEEPKSE"
     gene            complement(3128..4357)
                     /locus_tag="PL056_07395"
     CDS             complement(3128..4357)
                     /locus_tag="PL056_07395"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HPM46878.1"
                     /translation="MRMKLLVVFAFAITGFLFSGCENELGGFNLDEQTISFETFNVRL
                     GEELPLLSERTEKLERILKTADSDILCLQEIYDKNEIMKISQFLKDKKNDLGYISTVY
                     VNTKNEDFDPIPPSCTQEEVAPVVMCVAMECGGTDIACIAQNCLGDILALPPVCQQCL
                     IGDGLGSIAGGDFQAVLEECMVEKEVVYKNNGNNGILLASKYPLKDKGTLALTSYGNH
                     RMAVYATLGRIEKDGEIYDYKPAIGDTQIICTALSAIIDEEYDGIAGSWQQEQLTQIN
                     EILSIPDNENIAQRVIMGDFNSNIAGGENIIESNPGPVAALISDGWYDPYFDVEEEDI
                     IQCTVCPENPFINKNSSEAVHDHIFFRNRKGFVFMSERVFHNEFLLFNEKEKTKTRYS
                     ISDHYGIRTVMTVDPNF"
     gene            complement(4382..6124)
                     /locus_tag="PL056_07400"
     CDS             complement(4382..6124)
                     /locus_tag="PL056_07400"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HPM46879.1"
                     /translation="MKIELSLSLLLEMLEEYSVINKEQKELISARKDYYQSMFETTFK
                     RAPFVTELLVFTCEKESIKLSEEQLLRLIAARAGCEYVVIDPLKIDNQLTVKTISAAY
                     GKINRVIPLYKTGDDIVLAMADPFRMDIIDQLSTSTSSGIIPVVASAKDIERVLADLF
                     GFKKSIKAAADDLVREGFNNLENLTDLGKAKNMDDKHIINAVDYMLKYAIDQEASDIH
                     IEPKRDETNIRFRLDGILHTIYSFPFEAHLPFVSRLKMLAKMDIAEKRRPQDGRIKVV
                     TETGLEVELRASTVPVVTGEKMVLRVLESSTFVKDINNIGFSDEQKELYDVSLKKGYG
                     MVLVTGPTGSGKSTTLYSTLKWLASPEINVVSVEDPIEIIIDEINQMGVNVKAGITFS
                     SALRHILRQDPDIVMIGEIRDKETATNAVQAALTGHLVLSTLHTNDTATTIERIGDLG
                     IEPYLIASVLNAIVAQRLVRKVCPFCSFLREMSEEEKITLHLPLESTYKIRDAEGCVK
                     CRYTGYKGRTAIIEFMNITPKMRSIISEGATADEIRNNAIMDGMLTLKESAIRKLADG
                     VTTFDEIVKALYYE"
     gene            complement(6142..7722)
                     /locus_tag="PL056_07405"
     CDS             complement(6142..7722)
                     /locus_tag="PL056_07405"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPM46880.1"
                     /translation="MSRNLLVYEADVAMSEALNKLFSSYNFNVFEASSEGRVQEALGK
                     EKMDLFIIRAENPGMKGFMLCKKIRAMEEYANVPILLISSDAKEEVFERHRQFEYRAD
                     FYLKIPVDNETMLATTHAIFPFLEGIADIPVDSGGNGKQVAELKNRIAQLEKMLEEAD
                     KSSAGNELGEEVEALVQANQELTSKNEELQGVLAKLEEESGELKDAIAQKGFQIKGLE
                     EKISDLQNAAQESSESEKELKKLSKELENTEKEKEEFVSQIEKLEGTVSLLSEELEDI
                     KKENDELQDSVKTLKEAAEAKKEGESQEIADFKKKLAEQKDQNDVLSVYNKEMKDRVK
                     KIEKETADALKEKENAVNEMEKLKADLEQKITANEKEMNELRETVANLAEVAEELEKT
                     RMEKSDLEKNIADLKQEIEELTDAVSKSGELAEQFETVMKEKLEFEEKLNERRDRIKE
                     LNDELDKMSEKTEGMELVIAENEELKKKAASADSELEEKTKTLDELTAKTSQLETDYQ
                     KLSELMTKLKEIVSQFEG"
     gene            complement(7731..8201)
                     /locus_tag="PL056_07410"
     CDS             complement(7731..8201)
                     /locus_tag="PL056_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46881.1"
                     /translation="MKDKIESMIRKNNGAVEVINALSKDASFRVVMENDADIVISREG
                     GSFTVSNFKSGQSPDFTVFSTFDITSRIFNSCGRCSDVKEFLINSTKILMDKENRKHI
                     SLSVHKGFLTLTMHGYLKLLAMGGTQFIKTLADHGIDSVNTIRKKLTSLTNRLN"
     gene            complement(8281..9795)
                     /locus_tag="PL056_07415"
     CDS             complement(8281..9795)
                     /locus_tag="PL056_07415"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HPM46882.1"
                     /translation="MRFFRFLLISLSVPFFVLSCVTDENDILMDDENVISDKEENKND
                     EENQLLCGNKTVDSGEICDGGAKECLEIDSQYSGGIATCNSTCDGWITSDCKQNTGDT
                     GDTGNSGNTGDSGDSGDSGTDSFDKNAPGIWVDPETFLIWENPMGNKGAGGLGPKHAE
                     ALKYCENLVLAGATDWRLPTISELRTIARGVSTVMTGGKCPTTDTCTDQDTCNKDKEN
                     TQGFGNSCLGCYALDETKYDPDLAYLTADDCKLTDRQLDNEECYIVPILHGPCNGTWS
                     NTPNTSAAGTMASAYWYLNFKGGLINSDADSLSGANWVRCVRAGTADDVPENDYVPQN
                     PYGNCYFDSECEEGKWCENNNCVIIPAATFVDTNGLEWQAGKVETNTWQESLDYCEAL
                     DFAGFSDWRLPNIDELKSLVKGCAKTTACTVTSSCTGYTACNGGSSDCKTGCANGNYL
                     PAEVGQQNLMYWTSDEEAQATTNAWLISFSTGSVSYMKKTWDNQLARCVRGTKN"
BASE COUNT         2741 a         2060 c         2025 g         3030 t
ORIGIN      
        1 acaaggataa gatagatcag gaggtgacag ttggttcgta tcgcaggagt tgatcttccc
       61 aggaagaaaa gaatggagat cgcgctgacc tatatttacg gaataggcag gacaagtgct
      121 cagaaaatac ttgacgaagc gggtattgac aggagcacaa aaagtgatga tgtcgatgat
      181 tccatggcaa ttaaaataag ggccgtcatc gatcagaagt tcaaggtcga aggtgatctt
      241 agaaaagatg tcataatgaa cataaaacgg cttatggacc tcggcagcta cagaggtcgt
      301 agacacagaa gaggtctgcc gacaaggggc cagagaactc gtaccaatgc caggacaaga
      361 aagggtccaa ggcgtaacct tatgaagaag aagaaatagc aggaggtata gagatggcac
      421 cggaaaaaaa gaaaaaagtt aaaaaaagca tacagacagg actggcatat attcagtgca
      481 gttttaacaa cacgatcgtt acgatcactg atgtaagcgg aaatgtactg tcatggtcaa
      541 gcgccggtct taaaggattc cgcggaagta agaaaagtac tccctatgca gcacaggttg
      601 cagcggaaga tgccggcaaa aaggcacagg attacggttt gagaactctg agtgtttttg
      661 taaaaggtcc gggaagcgga agagaagcag ctatcagagg actctcaaat gtgggatata
      721 atgtaactct tctgcgggat ataacaccca ttcctcataa tggatgccgt cccaaaaaga
      781 aaagaagagt ataatcatta tttggtgtaa acttttactt acctaaaagg aggaagcttt
      841 ggctaagtac aaagaagcaa aatgtaagat ctgcagaaga gaaggagcga agctctttct
      901 gaaaggtgaa cgctgtcata ctgaaaagtg ttcatttgaa agaaaaccct atggccccgg
      961 caatcatgga aaggacaaga aaagagttac cgagtacaag attcagctga gggagaaaca
     1021 gaaagcaaaa gctatttacg gcatgcttga aaaacagttc agtatttttt atgacaggtc
     1081 tgtccttaag aaaggcgcaa ccggtgaaaa tcttttaaga atgcttgaat gccgtcttga
     1141 caacattgtt taccgtctcg gatttgcaaa cagcagagct caggcaagac agctgatcaa
     1201 ccacagacat ttttccgtga acggcagact taccgatatc ccttctttca ttgtgaaggc
     1261 gggagatgtt gttgttctta aggaaaaaag cagaaattcc aagattttca aggaaacaat
     1321 agaatctgcg ggagaaagac tccctgttcc ttcatggctt gaactgaaca aaggtgaatt
     1381 caaaggaaca gttgtacgac ttccgggaag agaagatata actgaagatg taaaagaaaa
     1441 ccttatcgta gagctttatt ctaagtaata gggggaattt aaatgtatcg agccaattgg
     1501 aaaacattga tcatgcccaa agatgtaagg gttgtagagc agggtgcgga tggtgcatat
     1561 gcccgttttg tctgcgaacc tcttatgaag ggttatggaa ttactctggg caattcgctt
     1621 cgaagaattc ttatttcaag tcttcgcgga gcagctgtaa tcggagttaa aattaaaggt
     1681 tacgagcatg aatttgcaac tattccggga ataaaggaag atgtggtgga tatcattctc
     1741 aacataaaac agatccggtt tgtcgtccat gaagataaac tgcacacgct gctccttagg
     1801 aaaaatgtaa aagggacagt tactgccgct gacatagccg aaacagccgg cataaagatc
     1861 ctcaacaaag acctcgtgct ttttgagatg gatgaaggag ttgattttga aatggaactt
     1921 attgtcggca taggaagagg atatgttccg tctgaagcac acaatcagga tatcttttcc
     1981 gagggctata ttgcagttga ttcatttttc tctcctgttg taagagcaaa ctataaagtc
     2041 accaatgcaa gagttaagaa cagttttgat tttgataaaa tggtctttga tcttgaaaca
     2101 gacggctctc tttctccacg cgatgcactt gcttatgcgg caatgatact cagggaacac
     2161 acaaagttct ttatcagttt ctcaattgat gaaaaggatg agttaaatgc ttcagattct
     2221 cacgaagagg tcaacatgaa cctttacagg acaattgatg aacttgacct gagcgtaaga
     2281 tcgtataact gtcttaaaaa tgcaaacatc cgttttatag gcgaacttgt aaagagaagc
     2341 gaagcggaaa tgcttaaaac caagaatttc gggagaaaat ctcttaacga aataaagata
     2401 cttctcataa agatgggatt acatttcgga atggatatag acaatttccc ggatcagaag
     2461 atcattgaac aaattgaaaa gaaatcaggg agcaagatat gagacacaga aaggcaggca
     2521 ggaaactgaa cagagacagt tcccacagag aggcaatgct ttgcaatatg cttaattctc
     2581 tggttaagaa cgaaagaatt caaacaactt tcggaagagc gaaggaactg cagaaaattg
     2641 cagataaagc tgtaacactc gcaaagaaga atacaacagc ttcgatcagt cagcttacta
     2701 agatcgttaa gtcagacaga gaagaagtca ttacaaaact tcttaaagag atcggaccta
     2761 gatttgcatc aagagccggt ggatatacaa gaataataaa agtcggtttt cgcagaggtg
     2821 atgcggctcc cactgtaata attgaatatc ttggtgctga tgagccggtt cagaaaaaga
     2881 caaagaagaa aaagacagtg aaaaaggaag ctccggttgt tgaagcagct ccggttgttg
     2941 aagcagttcc ggttgttgaa gcagttccgg ttgttgaaac tccggcagtt gaagaagctc
     3001 ctgttcctgt tgaagaagtt ccagtagcgg aagaagtcaa agaagagcca aaatcagaat
     3061 aagatcaata tcttaatatt aaaattcagg ggggctgaaa agcccctttt tttattttat
     3121 taaatatcta gaaattgggg tcgactgtca tcacggttct aataccgtag tgatctgaaa
     3181 tggaatatct cgttttggtt ttctcttttt cgttgaaaag gagaaattca ttgtggaaca
     3241 ctctttctga catgaaaaca aatccttttc tgtttctgaa aaaaatgtga tcgtgaacag
     3301 cttcactgct gtttttattg atgaatgggt tttccggaca cacagtacac tggatgatat
     3361 cttcttcttc aacatcaaaa taaggatcgt accatccgtc agaaatgagc gcggcaaccg
     3421 gccccggatt tgattcaatt atattttctc cgccagctat attgctgttg aaatcaccca
     3481 ttattactct ctgggcaata ttttcgttgt caggaattga aagtatctcg ttgatctgtg
     3541 tcaactgttc ctgctgccag ctcccggcaa taccgtcata ttcctcatca attattgcag
     3601 aaagtgctgt acagatgatt tgagtatctc ctatggccgg tttgtagtcg tatatttccc
     3661 catctttttc tattcttcca agcgtagcat aaacggccat tctgtggtta ccatatgatg
     3721 tcaatgcaag agtcccttta tctttaaggg gatattttga tgcaagaaga atcccgttgt
     3781 ttccattgtt tttataaaca acctcttttt caaccataca ttcttcaagt accgcctgaa
     3841 aatctcctcc tgctattgaa ccaagtccgt cgccgatgag acattgctgg cagaccggag
     3901 gaagagcgag aatgtcaccg aggcagtttt gagcaatgca ggcaatgtcg gtaccgccgc
     3961 attccattgc gacacacata acaacaggag ccacttcttc ctgagtacat gacgggggta
     4021 tcggatcaaa atcttcattt tttgtgttga catatacagt tgaaatatat ccgagatcgt
     4081 tctttttgtc tttaagaaac tgacttattt tcatgatctc attcttgtca tatatttcct
     4141 gaagacaaag tatatctgaa tctgctgttt taaggatcct ttcaagtttt tcagttcttt
     4201 cacttaaaag aggaagctcc tcgccgagac ggacattgaa tgtttcaaaa gatattgtct
     4261 gttcgtcaag attaaagccg ccaagttcgt tttcacatcc tgaaaaaagg aagcccgtaa
     4321 ttgcgaatgc aaacacgact aatagtttca ttctcatttg tttccccgta tgtagttaat
     4381 attactcata ataaagagct tttacgattt catcgaaagt tgtgactccg tcagccagtt
     4441 ttcttattgc agactcctta agtgtcagca ttccgtccat aatggcgttg ttccttattt
     4501 cgtcagctgt agccccttcg gaaatgatag acctcatttt aggagttatg ttcataaatt
     4561 ctatgatagc cgttcttcct ttatatccgg tgtatctgca ctttacacaa ccttccgcat
     4621 ctcttatctt gtaagtgctt tcaagcggaa gatgaagtgt tattttctct tcttcactca
     4681 tttcccgcag gaaagagcag aaggggcaaa cttttctaac aagccgctgt gcaacaatag
     4741 cgtttaaaac acttgcaatc agatagggtt caattccaag atcgcctatt ctttcaattg
     4801 ttgttgccgt atcgtttgtg tggagagttg aaaggacaag atgacccgta agagcggcct
     4861 gtacagcatt tgtggcggtt tccttgtctc tgatctcacc gatcataaca atatcaggat
     4921 cctgtcttaa aatatgtctc aaagcgcttg aaaaggttat tccggcttta acattaacac
     4981 ccatctggtt gatctcatca attattatct caatgggatc ttcaactgaa acgacattta
     5041 tttcagggct tgcgagccat ttcagagttg aataaagcgt tgttgatttt ccgcttcctg
     5101 tcggaccggt cacaagaacc atgccgtatc cttttttaag agaaacatca tagagctcct
     5161 tctgttcgtc tgaaaaccct atattgttga tatctttaac aaaagtagat gactcaagca
     5221 ctcttagaac cattttctcg ccagttacga ctggaacggt gcttgctctc agttcaactt
     5281 caagaccggt ttcggtcaca acttttattc ttccgtcctg aggtcttctt ttttccgcaa
     5341 tgtccatttt tgcgagcatt ttaagccggc tgacaaacgg aagatgggct tcaaacggga
     5401 atgagtatat tgtgtgcagt atcccgtcaa gccggaacct gatatttgtt tcatcccgtt
     5461 tgggctcgat gtggatgtcg cttgcctcct gatctatggc atatttgagc atataatcaa
     5521 ccgcattgat tatgtgcttg tcatccatgt ttttcgcctt gccgaggtcg gtaagatttt
     5581 caagattgtt aaatccttcc ctgacaagat catcggcagc tgcttttatc gatttcttaa
     5641 agccgaaaag atctgcaaga accctttcta tatcttttgc agacgccaca acgggaatta
     5701 ttcccgatga agtggaagtt gaaagctgat cgataatgtc catcctgaaa gggtctgcca
     5761 ttgcaagaac gatgtcgtct cctgttttat acaaggggat gactctgtta atttttccgt
     5821 atgccgcact tattgttttc acagtaagct gattgtctat ttttagcggg tctatcacaa
     5881 catattcgca tcctgctcta gctgcgataa gacggagcag ttgttcctca gaaagtttaa
     5941 tagattcttt ctcgcaggtg aacacaagga gttctgtaac aaaaggggcc cttttgaaag
     6001 tggtctcaaa cattgactga tagtaatctt ttctggcaga aataagttcc ttctgctctt
     6061 tatttattac tgaatattct tcaagcattt caaggagcag tgaaagagaa agttcaattt
     6121 tcatcgattc tccggaaatg attaaccctc aaactgtgaa actatttcct tgagttttgt
     6181 cattaactca ctgagtttct gataatctgt ttcaagctgg gaggtttttg cggtaagctc
     6241 atcaagcgtt ttggtctttt cctcaagttc agagtctgca gaagcagctt ttttcttcaa
     6301 ctcttcattt tcagcaatga caagttccat cccttccgtt ttttcggaca tcttgtcgag
     6361 ttcgtcatta agttctttga tgcggtctct tctctcatta agcttttctt caaattcgag
     6421 tttttctttc atgaccgttt caaactgttc tgcgagctct ccgctttttg aaacagcatc
     6481 tgtaagttct tcaatttcct gtttaaggtc agcaatattt ttctcgagat ccgatttttc
     6541 catccttgtc ttttcaagtt cttcagcaac ttcggcaaga tttgcaacag tttccctgag
     6601 ctcgttcatc tctttttcgt ttgctgtaat tttctgttca agatcggctt tcaatttttc
     6661 catttcgttc acagcatttt ctttttcctt caatgcgtcg gcagtctctt tttcaatttt
     6721 tttgacacgg tctttcattt ccttgttgta tacggaaaga acatcgttct gatctttctg
     6781 ttctgcaagc tttttcttga aatcggctat ttcctgagat tcgccttctt tttttgcttc
     6841 agccgcctct ttcaaagttt ttactgaatc ttgaagttca tcattctctt ttttaatatc
     6901 ttcaagttct tcacttagca gagatacggt cccttcgagt ttttctatct gtgaaacgaa
     6961 ctcttctttt tctttttctg tattttcgag ctcctttgag agtttcttaa gttctttctc
     7021 tgattctgag gattcttgag ccgcattttg aagatcagat atcttttcct caagaccttt
     7081 tatctgaaat cctttctgag caatggcatc tttgagttct cccgattcct cttcaagttt
     7141 tgcaagcact ccctgaagtt cttcgttttt gcttgtcaac tcctgatttg cctgaacaag
     7201 cgcttcaact tcttcgccca gttcatttcc tgctgatgat ttatctgcct cttcaagcat
     7261 tttctcaagc tgagcaatcc tgtttttcag ttcagcaacc tgttttccat tgccgcctga
     7321 atcgacagga atatcagcta tcccttcaag gaaagggaaa atggcatgag tggttgcaag
     7381 cattgtttcg ttgtcaacag ggatcttgag atagaagtcg gctctgtatt caaactgtct
     7441 gtgtctttca aaaacttctt ctttggcatc acttgaaata agaaggatcg gcacatttgc
     7501 atattcttcc attgccctta tctttttgca gagcatgaat cccttcatcc cgggattttc
     7561 cgcacggatt atgaaaaggt ccattttttc ctttccgagt gcttcctgaa ctcttccttc
     7621 agagcttgct tcaaaaacat taaagttgta ggatgaaaaa agtttgttca acgcctctga
     7681 cattgcaaca tcagcttcat agacaagtag gtttctgctc ataatggcct ctaatttaat
     7741 ctatttgtaa gacttgtgag ttttttcctt atggtattta cggaatcgat cccgtgatct
     7801 gcaagcgttt tgatgaactg tgtccctccc attgcaagaa gtttaagata tccgtgcatg
     7861 gtgagagtca gaaacccttt gtggacagaa agggagatgt gttttctgtt ttccttgtcc
     7921 attaaaatct tagttgaatt tatcagaaat tcttttacat cactgcatct gccgcatgaa
     7981 ttaaagatgc gcgaggtaat gtcgaatgtt gaaaatacgg tgaaatcagg gctctgtccg
     8041 gatttgaaat ttgaaactgt gaaagaacca ccttctcttg atatgacgat gtctgcatcg
     8101 ttttccatta caactctgaa tgatgcgtcc tttgaaagtg cgtttatcac ttcaacggca
     8161 ccattgttct ttctaatcat cgactcaatc ttgtctttca tttcaaacct aatcaccaac
     8221 agattgaaaa acttatcaca aggcatattt ttttgcaaat aaattaatga agataataaa
     8281 tcagttcttt gttccccgga cgcatcttgc aagctgattg tcccaagttt ttttcatata
     8341 actgaccgac ccggtcgaaa aacttataag ccatgcattt gttgttgcct gtgcttcttc
     8401 gtcacttgtc cagtacatta aattctgctg gccgacttct gccggaagat aatttccgtt
     8461 tgcacagccc gttttgcagt cactgcttcc gccgttgcag gcagtgtatc cggtgcagga
     8521 tgaagtgaca gtgcatgccg ttgtttttgc acaccctttt acgagtgatt taagttcatc
     8581 gatattcgga agccgccagt ctgaaaatcc cgcaaaatcc agagcttcgc agtaatcaag
     8641 cgattcctgc catgtgtttg tttccacttt tcccgcctgc cattcaagac cattcgtatc
     8701 tacaaaagtt gccgcaggaa taattacaca gttgttgttc tcgcaccatt tcccttcttc
     8761 acattcgctg tcaaaataac aatttccgta aggattttga gggacataat cgttttccgg
     8821 aacatcgtcg gcagttccgg ctctgacaca tctgacccag tttgcgccgc ttaatgaatc
     8881 tgcatcactg ttgataagcc cccctttgaa attgagatac cagtaggcac ttgccattgt
     8941 tcctgccgca ctggtgttag gggtatttga ccatgttccg ttgcatggtc cgtggagtat
     9001 cggcacgatg taacattctt cgttatcgag ctgtcgatct gtaagtttgc agtcatcagc
     9061 agtcaaatat gcaaggtcag gatcgtattt tgtttcatca agagcataac atccaagaca
     9121 cgaattgccg aaaccctgag tgttttcctt gtctttattg caggtgtcct gatctgtgca
     9181 ggtgtctgtc gtggggcatt ttccgccggt catgacagtg ctcacccccc ttgcaatagt
     9241 ccgcagctcg ctgattgtgg ggagccgcca gtctgttgcc ccggcaagaa cgagattttc
     9301 gcaatatttc aaggcctctg catgtttcgg gcctaaaccg cccgctcctt tattgcccat
     9361 cgggttttcc cagatgagga atgtttcagg atcgacccag atgcctggag cgttcttgtc
     9421 gaaactgtca gttcccgaat caccggaatc accgctgtca cctgtatttc ctgaatttcc
     9481 ggtatcaccc gtatcacctg tattctgttt gcagtcagaa gttatccacc cgtcgcaggt
     9541 cgaattgcat gtcgctattc cccctgaata ttgagagtca atttctaaac attccttcgc
     9601 tccgccgtcg caaatctcgc cgctgtcaac tgttttgttg ccgcacaaaa gctgattttc
     9661 ctcatcattt ttgttttctt ctttatcgga aatgacattt tcatcatcca tgagaatatc
     9721 attttcgtct gtaacacaac tcaaaacaaa gaacggaaca gagagtgaaa taagtaaaaa
     9781 ccggaaaaaa cgcatgaccg cctccaaaaa aattaatgac caacaaaaaa atgatgtacc
     9841 tttaaaaaat taattc
//
LOCUS       DAOOYB010000103         9659 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_7685_length_9659_cov_14.843919, whole genome shotgun sequence.
ACCESSION   DAOOYB010000103 DAOOYB010000000
VERSION     DAOOYB010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9659
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7685_length_9659_cov_14.843919"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1346
                     /locus_tag="PL056_07420"
     CDS             <1..1346
                     /locus_tag="PL056_07420"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HPM46883.1"
                     /translation="ILSSLGFAIFTTWPFKSGRNIRKYLYHLMIKSLARLVILSAPQV
                     RLRKNNPHNEDFKKPAVIISNHSSFLDILMMLSLQVKIVMVTKKWVSSNPVFGKLVQF
                     ADFFTTTEGFEKMAPKLQKCIDAGYSVVVFPEGTRGDGINLQRFHKGAFFLADQLKLD
                     IVPIVIHGAGHSIKKGEFSVRGSTLSYNILKRIPADSTEYGETYQEKCRNISAMFKRE
                     YESVYEKLGTVNFYRDRLMKNYIYKGPDIEWYARIKIMLEDNYRFFNNIIPRKAEIYD
                     IGCGMGMLTFMLTLTSKERSVKAIDYDETKINLAKNCYSCSERTTFICDDVTKTAFEK
                     ADVFIMNDVLHYLPVEEQSALIEKCSGKLNENGIIIIRDADSGMSGKHGNTVVTEKFS
                     TGLKFNQADTGLNFVSRSVIEKAAVSAGLSFEVVKHQKKTSNVVYLLKKQGVDDV"
     gene            1339..2814
                     /locus_tag="PL056_07425"
     CDS             1339..2814
                     /locus_tag="PL056_07425"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPM46884.1"
                     /translation="MYDFAIIGSGLGGLICAYILSKEGKKVCVVEQHFKPGGSLQTFK
                     RDGRIFDTGVHYIGGLEKGQNLYNYFKYLKIMDDLKIRKLDGNAFDRICFNGNGKEYP
                     LAMGYDNFVEQLSFHFPKEREGIKKYIEFTKNMVSAFPLYNLSTKAAEIPEEFFFQKN
                     AKEVIESFTSDPLLQNVLAGNNPLYAGVAEKTPIHVHALINDNFIKSSWRMVDGGSQI
                     ADALIENIKKNGVEIKTNSKVVKLAAEKKELKYIETQNGERIYASNFISTMHPADMLD
                     MLEGDSINPSYREKIYSINDTVGVFGLYITLKENSFEYRNSNYYCYSEESAWTSDWKK
                     SYMFITPAISKSEKYADSAIALSYMDINEVAKWKDSKYLKRGSDYEEFKQVRAEILLD
                     QISKKFPGFRSSVEKVYSSTPLTFRDYVGARNGALYGVLRDCNEPHKSFISPKTRVKN
                     LYLSGQNIILHGILGVTIGSILTCGSILGFEYLLEKIRNEV"
     gene            2804..4297
                     /locus_tag="PL056_07430"
     CDS             2804..4297
                     /locus_tag="PL056_07430"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HPM46885.1"
                     /translation="MKFEKTKKIKRLVIFSLLFSPIIFVAFLLLLTSAEIPVEIKDQI
                     IPVAVAEKIDGSLSGIKNNRFRHSQSGLYELYVEGSPIEIGRYEGVLTKELIVRQEEI
                     FVDMLNRFVPGKYSRFMMKKFIYLFNKSMQKEISEEYLSEIFGISSVMEDDFAGLGNP
                     YFRVLNYHAAHDIGHMMTNLNLVGCTAFSVKGEKSVDGDLITGRNFDFFIGDRFAKEK
                     IISFIKPDNGFPFATVSWGGMTGVISGMNLRGLSITLNAAESEIPTDTATPVVLVARE
                     MLQYADTIEKAYEIARSREIFVSQIFFVSSAIDNRSALIEKTPEKTELFYPESDESAV
                     TNHFTGKLKGSEKHRNPESLRNSQKRLERVYELIEKEKPLNIEKTVKILRDTKGYGDK
                     QVEPGSETAINQYVAHHSVVFNNTKKIMFISTSPWQFGEFRAYELEKVFNEFSKLRND
                     VEISDEELTIPADEFMKSEEFQRVKKSRDDGTFKKDDIRFSKDGLNM"
     gene            4307..5557
                     /locus_tag="PL056_07435"
     CDS             4307..5557
                     /locus_tag="PL056_07435"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPM46886.1"
                     /translation="MVKKIADANFLKTLDYCMKRSKFYQRHFRNVDLSKIKGIDDISK
                     LPFTTKDDIAEFSEDFQCVADKEIVEIATTSGSTGRPLMLKYTKKDLERLAFNEHLAF
                     HNAGLDENDKVLISVTIDKCFIAGLAYYIGLNSIGAASVRVGPRSPELLIKMAEVTSP
                     TAIIGVPTYLDKVFDIAAEEGNESVFKTVKKIICIGEPVKEKDMSLNAIGKRLNQKYK
                     AGIFSTYGITELAISFCECRLSKGGHIHPELIHAEIVDDEGNPVPDGVEGEVVATTFN
                     VEGMPLIRFKTGDISFIVDDGLCGCGLKSKRLGPVTGRKKQKLKIKGTSVYPQMIINV
                     MEEIDYVDNFVLIATSDTTLSDHLEVRCSLKPGISDRTKDIMAKLKGNLKVTPKVVLD
                     SNANIDLIQSSVSKRKKTKFVDRR"
     gene            5578..6438
                     /gene="panC"
                     /locus_tag="PL056_07440"
     CDS             5578..6438
                     /gene="panC"
                     /locus_tag="PL056_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018002301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HPM46887.1"
                     /translation="MKVLKTVDEMRSFRKDSAGGLGFVPTMGALHEGHCELIKRSASE
                     CERTVVSIFVNPTQFGPNEDFKKYPRTLDEDLKKCEISGADAVFVPEVNTIYGESAPL
                     ITLKAGGEMTSVLCGKSRPGHFDGVVQVVSILFNIVQPDISFFGEKDFQQLAVIRKMV
                     SDLHFPVEIKGVATVRESDGLAKSSRNRYLSEKERVLASNIFKVLESLSQKAESARFD
                     RLAIPVEFAEEEAKKRLESLVAGIKIDYFEIRNSSDLSKTRFLGKDSRVFVAAYIGTT
                     RLIDNLFIGE"
     gene            6440..7588
                     /locus_tag="PL056_07445"
     CDS             6440..7588
                     /locus_tag="PL056_07445"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPM46888.1"
                     /translation="MVRFSESITKAGTYPFKKVTDLKNGLIKKGVKVIDLGMGDTTLP
                     APEIAIEAVQKAVADPANHHYNFYNGYPFFREAASSWMERKHGVKLDPEKEITAVIGT
                     KEALFRMPSSFIDPGDIAIIPDPSYPPLESGTLSAGGGVHFVPLRHQNGFMIDFCEIP
                     ENIKQKAKFIYINYPNNPTSAVMTDAFAKELLKQAQIYDWAILSDMAYSEVYENRRNV
                     SLLEFEGAKERVIEFHSLSKTFSMTGFRIGFACGNSEMVSSLVKIKSIRGSSPFQPVQ
                     AAGAAVMENGDGYLLTMREFYKKNRMLLKSIFAKKGLEIFNSESTFYVWAKVPGSMTS
                     LEYSTLLVEKYGTVVTPGSILGAGGEGWFRACFACRTEDILEFERRFI"
     gene            7591..8124
                     /gene="folK"
                     /locus_tag="PL056_07450"
     CDS             7591..8124
                     /gene="folK"
                     /locus_tag="PL056_07450"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HPM46889.1"
                     /translation="MKYLLSIGSNIGCGMKNLKKAVTMLEERSIFVDECSSVYCSSPV
                     DYLSQADFMNVSVIAETEFSPEDLLKKLKEVENEMGRVKTIEKGPRNIDLDIVFWEKG
                     SHSSKNLTIPHKEAVKRLFVIFPTLEIIENSEHFENEKRELQTVLEVEKDRFTGQRIE
                     KLCPFIMENVTYGWKDS"
     gene            8105..8380
                     /locus_tag="PL056_07455"
     CDS             8105..8380
                     /locus_tag="PL056_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46890.1"
                     /translation="MAGKIVDRFLDKVPFSNEIIDVLKDHIDLKFTKQLAIEIVHQVS
                     GQGDKLKKEIASIISKEIQREFNRVDKSEILQKALEGLEITISFKKK"
     gene            complement(8381..9274)
                     /locus_tag="PL056_07460"
     CDS             complement(8381..9274)
                     /locus_tag="PL056_07460"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HPM46891.1"
                     /translation="MFFSGKERFFTDRLYSARWFRSYFLIVLGSAIMSAGYSFFADPH
                     KIVPGGVYGTAIVIHHTFGLPTGTVGLIFNIPLFIAGIYILGPRFGVKTFVGTILTSV
                     FIDVFNSLNWGTPVEDKMLASIVAGVMIGTGLALIFKSKATTGGSDIIAQITNKYTKM
                     PVGQLLIIIDSLVVSVGVIAFKDVSLALYALITIYITGKVLDGVLLGSNNRKAVFVIT
                     DKHEEVREFILKKLNRGGTYFYGQGMYEKSEKKIVFTALSRRELAALQDFVKDVDSEA
                     FISVFDTNQIYGHGFIPIEES"
     gene            complement(9415..>9659)
                     /locus_tag="PL056_07465"
     CDS             complement(9415..>9659)
                     /locus_tag="PL056_07465"
                     /inference="COORDINATES: protein motif:HMM:NF018009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HPM46892.1"
                     /translation="TRKLDARDVGQMNKYVSYFRINRQYEHEKETIGLIICSEAGIEE
                     AQYALGGLEEKIFIATYQPKLPTMEQLKKAVKRATE"
BASE COUNT         3140 a         1775 c         2140 g         2604 t
ORIGIN      
        1 tgatcctttc ttcgctcggt tttgcgatat tcaccacatg gccttttaaa agcggtcgca
       61 acataagaaa atatctttac catttgatga taaagtctct tgcccggctg gtcatccttt
      121 cagctccgca ggtacggctg agaaagaaca atccccataa cgaagatttt aagaaaccgg
      181 ctgtaattat ctcaaatcat tcatcttttc ttgatatact gatgatgctt tcgttgcagg
      241 tcaagatagt aatggtgaca aaaaaatggg tttcaagcaa cccggtattc gggaaacttg
      301 tccagttcgc cgactttttc acgacgactg aagggtttga gaaaatggca cccaaactgc
      361 agaaatgtat tgatgccgga tattcggtcg tagtattccc ggaagggaca agaggggacg
      421 gcataaatct tcaaagattt cataagggtg cgtttttcct tgcagatcag ctcaaacttg
      481 acatagttcc aattgtcatt cacggggcgg ggcacagcat taaaaaaggg gaattctcag
      541 tgcgcgggag caccctttcc tataacattt taaaaaggat ccctgctgat tcaactgaat
      601 atggtgaaac ttatcaggaa aaatgcagga acatttcagc aatgttcaaa agggaatatg
      661 agtcggttta tgaaaaactc ggaacagtca atttctatcg tgaccggctg atgaaaaact
      721 atatttacaa gggtcctgac atcgaatggt atgcgagaat aaagataatg cttgaagata
      781 attacagatt tttcaataac attattccac ggaaagcaga gatctatgac atcggctgcg
      841 gaatgggaat gcttacattc atgcttacgc tcacttcaaa ggaaagatct gtaaaagcga
      901 tagattatga cgaaacgaaa ataaatttag caaaaaactg ttattcatgc agcgaaagga
      961 cgacttttat ctgtgatgat gttacaaaaa ccgcatttga aaaagccgat gtgttcataa
     1021 tgaatgatgt gcttcattat cttccggttg aagaacaatc cgccctgatt gagaaatgtt
     1081 ccggaaagct taatgaaaac gggatcataa taataaggga tgcggatagc ggaatgtccg
     1141 ggaaacatgg aaacacagtt gtgacagaaa aattttccac agggcttaaa ttcaatcagg
     1201 cggacacagg gctcaatttc gtttcaaggt ccgtaataga aaaagcggcg gtttcagcag
     1261 ggctttcatt tgaagtcgta aagcaccaga aaaaaacatc caatgtggta tatctcctta
     1321 aaaaacaagg agttgatgat gtatgatttt gctataatag gttcagggct gggggggctg
     1381 atctgtgcct atatcctttc aaaggaagga aaaaaggtgt gtgtcgtcga acagcacttc
     1441 aaacccggcg gatcactgca gacatttaaa agggatggaa ggatatttga taccggcgtc
     1501 cattacatcg ggggccttga aaaaggacaa aatctttata actactttaa atacctaaag
     1561 ataatggatg accttaaaat aaggaaactt gacgggaatg cttttgacag gatctgtttc
     1621 aacggcaacg gaaaagagta tccccttgca atgggttatg ataattttgt tgaacagctg
     1681 agtttccatt ttccaaaaga aagggaaggg ataaaaaaat acattgaatt cacaaaaaac
     1741 atggtctcgg cttttcctct ttataatctt tcaactaaag ccgctgaaat ccctgaagaa
     1801 ttctttttcc agaaaaatgc taaggaagtt atcgaaagtt ttacttcaga tcctctcctt
     1861 cagaatgttc ttgcagggaa caaccccctt tatgccggag tggcggaaaa aacacccatc
     1921 catgttcacg ctttgataaa cgacaatttc ataaaaagtt catggagaat ggttgacggc
     1981 ggctctcaga tagctgatgc cctgatagag aacataaaaa agaacggtgt ggaaataaag
     2041 acaaattcaa aagttgtgaa actcgctgct gaaaaaaaag agctgaaata tattgaaacg
     2101 caaaacggtg aaaggatata tgcatccaat tttatttcaa ctatgcatcc tgccgacatg
     2161 cttgacatgc ttgaagggga ttcgatcaat ccgtcataca gggagaaaat atactcgata
     2221 aatgatacag tgggggtttt cgggctctac atcactttga aagagaacag ttttgaatac
     2281 agaaattcaa attattactg ctactctgaa gaaagtgctt ggacttctga ctggaaaaaa
     2341 tcatatatgt tcattactcc ggctatttca aaaagtgaaa aatatgccga ttccgcaata
     2401 gccctttcat atatggatat aaacgaagtt gcaaaatgga aagactcgaa atatctgaaa
     2461 agaggcagtg attacgagga gttcaagcaa gttagagccg agattctgtt agatcagatc
     2521 tcaaagaaat tccccggatt cagatcgagt gttgaaaaag tatattcatc cacgccgctc
     2581 actttcaggg attatgtcgg ggcaaggaac ggagctcttt acggagtttt aagggactgc
     2641 aatgagccgc acaaatcttt catttcgccc aaaacgaggg tgaaaaacct ctacttaagc
     2701 ggtcagaaca taattcttca cggaattctc ggagttacga taggttcgat cctcacttgc
     2761 ggatcaattc tcggatttga gtatcttctg gaaaaaataa gaaatgaagt ttgaaaaaac
     2821 taagaaaata aaaagacttg tcatattttc actgcttttt tctccgatta tctttgttgc
     2881 ttttctttta ttgctgacct cggctgaaat tccggtagaa ataaaagatc agataatccc
     2941 ggttgcggtt gctgaaaaaa ttgatggatc tttgagcgga attaaaaaca accggttcag
     3001 acattctcaa agcgggctgt atgaacttta tgttgaagga agtccaattg aaatagggag
     3061 atatgaaggg gttctgacaa aagaacttat tgtcagacag gaagagatat ttgttgatat
     3121 gcttaacagg tttgtgccgg gcaagtactc gagatttatg atgaaaaagt tcatctatct
     3181 tttcaacaaa tcaatgcaga aagaaatatc agaagagtat ctgtcagaaa ttttcggaat
     3241 ttcatcagta atggaagacg attttgcagg actcggcaat ccttatttca gggtgcttaa
     3301 ttatcatgcc gctcatgaca tcgggcatat gatgacaaac cttaatcttg tagggtgtac
     3361 cgctttttcg gtcaaagggg aaaaaagtgt tgacggtgat ctgataaccg ggaggaattt
     3421 tgattttttc ataggtgaca gattcgctaa agaaaagatc atttcattta taaagccgga
     3481 caacggcttt ccctttgcaa cagtttcatg gggaggaatg acaggggtta tatcgggaat
     3541 gaatttaagg ggtttaagca taactctcaa tgcggctgaa agcgaaattc cgacagatac
     3601 cgccactccg gttgttcttg tcgcccgtga gatgctccaa tatgccgaca caattgaaaa
     3661 agcatatgaa attgcaagat caagagaaat atttgtaagt cagatatttt ttgtcagttc
     3721 cgcaattgac aacagatcgg cattaattga aaaaacccct gaaaaaacgg agctttttta
     3781 tccggaaagt gatgagtcgg cagtgacaaa tcattttacc ggaaaactca aagggagtga
     3841 aaaacacaga aaccccgaaa gtttgagaaa ctctcagaaa cggcttgagc gggtatatga
     3901 acttattgaa aaagaaaaac cgcttaatat tgagaaaaca gtaaaaatat taagagatac
     3961 aaaaggttat ggcgacaaac aagttgagcc gggaagtgaa actgcaataa accagtatgt
     4021 cgcccaccat tcggttgttt tcaataacac gaaaaaaata atgttcattt ccacttctcc
     4081 atggcagttc ggcgagttca gggcttatga actggaaaag gttttcaatg aattttccaa
     4141 acttcgtaat gatgttgaga tatcagatga agaattgaca ataccggcag atgaatttat
     4201 gaaaagtgaa gaatttcaaa gagttaaaaa gtccagagat gacgggacat ttaaaaagga
     4261 tgatatccgt ttttcaaagg acggactgaa tatgtgaggg agaaaaatgg taaaaaagat
     4321 cgctgatgca aattttctca aaacacttga ttactgtatg aaacgctcaa agttctacca
     4381 gagacatttc agaaatgtag atctttcaaa aataaaaggg atagatgaca tatcaaaact
     4441 cccttttaca acaaaagatg atattgccga gttttctgaa gattttcagt gtgttgccga
     4501 taaagagata gttgagatcg caacaacgag tggttcaacc ggcagaccgc tgatgcttaa
     4561 atacacaaaa aaagatcttg agcggcttgc ttttaacgag catctcgcct ttcacaacgc
     4621 cggacttgac gaaaacgaca aagttctcat aagtgtcacg atcgacaagt gtttcattgc
     4681 aggacttgca tattatatcg ggcttaattc gatcggtgcg gcaagtgtga gggtcggacc
     4741 gagatcaccc gaacttctta taaaaatggc ggaagtgact tctcccacag cgatcatcgg
     4801 tgttcccacc tatcttgaca aggtttttga cattgccgcg gaagaaggga atgaatcggt
     4861 ttttaaaaca gtaaaaaaaa ttatctgcat aggcgaaccg gtgaaagaaa aagacatgtc
     4921 gctcaacgcg ataggaaaac ggctgaatca gaaatacaag gccggaatat tttcaactta
     4981 cggaataact gaactcgcca tctcattttg tgaatgccgt ctttcaaagg ggggacacat
     5041 tcatcccgag ctgatacacg cagaaatagt tgatgatgaa ggaaaccctg ttcctgacgg
     5101 ggttgaagga gaggtcgttg caacaacttt caatgtcgaa gggatgcctc ttatcagatt
     5161 taaaacaggg gacatctctt ttattgtgga cgacggactt tgcggctgcg gacttaagtc
     5221 aaaacgactt ggacctgtta cgggaaggaa gaagcagaag ctcaagataa aaggaacttc
     5281 ggtttatcct cagatgataa taaatgtcat ggaagagatc gattatgttg ataattttgt
     5341 tcttattgct acaagcgaca caaccctttc agaccatctt gaagtgagat gttcgcttaa
     5401 gccgggaatt tctgacagaa caaaggatat catggcaaag ctcaagggaa atctgaaagt
     5461 gacacctaaa gttgtgcttg attcaaatgc gaatattgac cttattcagt catcagtttc
     5521 aaagaggaaa aaaacaaaat ttgttgacag aagatgaaat taaaaccgga gaaacagatg
     5581 aaagttctta aaaccgttga tgaaatgagg tcgtttagaa aagattctgc agggggtctc
     5641 ggttttgttc cgacaatggg tgctctccat gaagggcact gcgaactcat aaaaagaagt
     5701 gcttcagaat gtgaaagaac tgtggtgagc atttttgtaa atccgacaca gttcggtccg
     5761 aacgaagatt tcaaaaaata tccaagaacc cttgatgaag atcttaaaaa atgtgagatc
     5821 tcaggagctg atgcggtctt tgttccggaa gtgaacacga tatatggtga gtcggctcct
     5881 ctcataactt tgaaagcagg cggagagatg acatctgttc tttgcggaaa aagcaggccg
     5941 ggacattttg acggagttgt tcaggttgtg tccattcttt ttaatatcgt tcagccagac
     6001 atctcttttt tcggtgaaaa ggattttcag cagcttgcag taataagaaa aatggtgtca
     6061 gacctccatt ttcctgttga aataaaggga gttgcaacag taagagaatc tgacggactt
     6121 gcaaaaagtt caaggaatcg ttatctcagt gaaaaggaaa gagtgttggc ctccaatatt
     6181 tttaaagttc tcgaatcatt aagccagaaa gctgaatcgg caaggtttga ccgtcttgcc
     6241 atacctgttg aatttgctga agaagaagca aaaaaacgac ttgaatcact cgtggctggg
     6301 ataaagatag actactttga aataagaaac agcagcgacc tttcaaaaac ccggtttctt
     6361 ggaaaggatt caagggtttt tgtagcggcg tatatcggaa caacccgtct gatagataat
     6421 ctttttatcg gagaataaag tggtcagatt ttcggaaagc ataacaaaag cagggacata
     6481 tccttttaaa aaagttacag atcttaaaaa cggacttata aaaaaagggg tgaaagtcat
     6541 tgatctgggc atgggggaca caactcttcc ggcacctgaa attgcgatcg aagcagtgca
     6601 aaaagctgtt gccgaccctg caaatcatca ttacaacttc tataacgggt atcctttttt
     6661 cagggaggcc gcatcaagct ggatggaaag aaaacacggc gtgaaacttg atcctgaaaa
     6721 agaaataact gcggtaatag ggacaaaaga agcccttttc agaatgcctt cctctttcat
     6781 tgatccgggc gacattgcaa taattcctga tccctcgtat cccccacttg aatcgggaac
     6841 tttgtcagca ggcggcggtg tccattttgt accgcttaga catcagaacg gattcatgat
     6901 agatttttgt gaaatacctg aaaacattaa gcagaaagca aagttcattt atatcaatta
     6961 tccgaacaat ccgacatctg cggtaatgac agatgcgttt gcaaaagaac ttcttaaaca
     7021 ggcacagatc tatgactggg caatactttc agacatggct tattcagaag tctatgaaaa
     7081 cagaaggaat gtttctctgc ttgaatttga aggggcgaaa gagcgggtga tagagttcca
     7141 ttcactttca aaaaccttca gcatgacagg tttcaggatc gggtttgcat gcggaaacag
     7201 tgaaatggtc tcttctctag tcaagatcaa gtctatccgc gggtcatcac ctttccagcc
     7261 ggttcaggct gcgggggcgg cagtgatgga aaatggagac gggtatcttt tgacaatgag
     7321 agaattctac aaaaaaaaca gaatgttgtt aaaatcaata tttgcaaaaa aaggtcttga
     7381 aatttttaat tcagagagta cattttatgt gtgggcgaaa gtccccggtt caatgacatc
     7441 gcttgaatac agcactctgc ttgttgaaaa atacggcacg gtcgtgacac cggggtcgat
     7501 actgggagca ggcggcgaag ggtggttcag agcgtgtttt gcctgccgga ctgaggatat
     7561 tctcgaattt gaaaggagat ttatctgaaa atgaaatatc tgctctcgat cggttcaaat
     7621 atcggttgcg ggatgaaaaa tcttaaaaaa gcggtgacga tgcttgaaga gagatcgata
     7681 tttgtcgatg aatgttcaag tgtttactgt tcttctcctg ttgactatct ttcacaggcc
     7741 gattttatga atgtttcggt cattgccgag acggaattct ctccggaaga tcttcttaaa
     7801 aaactgaaag aggttgaaaa cgaaatggga agggtcaaga caattgaaaa aggtccgcgt
     7861 aacattgatc ttgacattgt tttctgggaa aaaggttctc acagttcaaa aaatcttacg
     7921 atacctcaca aggaagcagt aaaaaggctt tttgtgatct ttcccacact tgaaataatt
     7981 gaaaattctg aacatttcga aaatgaaaag agagaacttc agacagttct tgaagtggaa
     8041 aaggaccgtt ttacaggaca gaggatagaa aagctctgcc cgttcattat ggagaatgtg
     8101 acatatggct ggaaagatag ttgaccgttt tcttgataag gttccgtttt caaacgagat
     8161 aattgatgtt ctgaaagatc atatcgatct caagttcaca aaacagcttg caatagaaat
     8221 agttcatcag gtttccggac agggagacaa actgaaaaaa gagatcgctt cgataatttc
     8281 aaaagagata cagagggaat ttaacagggt tgacaagtcg gaaatccttc agaaagcact
     8341 tgaaggtctt gaaataacga taagtttcaa gaaaaagtga tcagctttcc tctatcggga
     8401 tgaatccgtg accgtatatc tgatttgtat caaaaacaga aatgaacgct tcactgtcaa
     8461 catctttgac aaaatcctgc agagcggcaa gttcgcgacg gcttaaagca gtgaaaacta
     8521 tctttttctc agatttttca tacattccct gaccatagaa gtaagtgcct ccgcggttaa
     8581 gttttttgag aatgaactcg cgcacttctt catgtttatc agtaatcacg aaaacagctt
     8641 tccggttgtt gcttccaagg agaactccgt caagaacttt gcctgtgata taaattgtga
     8701 tcagagcata gagagcaagc gagacatctt taaaagcgat gacacccact gaaacgacaa
     8761 gggaatcaat gattataaga agctgaccca caggcatttt ggtgtattta ttggttatct
     8821 gggcgattat gtcgcttccg cccgttgtcg cctttgactt gaatatcaat gcaagtcccg
     8881 ttccgatcat gactcctgct acaatgcttg cgagcatttt gtcttcgacc ggcgttcccc
     8941 agttgagcga attgaaaaca tcaataaaaa cagatgtaag tatcgttccg acaaaagttt
     9001 ttacaccgaa tctcgggccg agaatgtata ttcctgcaat gaaaagggga atgttaaata
     9061 tcaatccgac cgtacctgtc gggagcccga aagtatggtg gatcactatc gcagtgccgt
     9121 aaacaccacc cggaacgatc ttgtgcggat cggcaaaaaa cgaataacct gccgacatta
     9181 ttgccgaacc tagaacaatt agaaaatacg acctgaacca tcttgcagaa taaagacggt
     9241 cagtgaagaa ccgctctttt cctgaaaaaa acatgacttt ctccatcgat ctgaaaataa
     9301 cgagtgaacg ccacctctat atacacagag aaaaattagt gtcaagagat atcggtttgt
     9361 gatgcggtat gttaatgata ggaaaccgtt atgtgcccgt ttgaggaata gcttttattc
     9421 cgtcgctctt ttcacggctt ttttcaactg ttccatcgtc ggaagtttcg gttgatatgt
     9481 agcgataaat attttttcct caagtccgcc aagagcatat tgggcttctt caattccggc
     9541 ttctgagcag ataataagtc caatggtctc tttttcatgc tcatactggc gattaattct
     9601 aaaataactg acatatttat tcatctgtcc aacatctctt gcatcaagtt tccttgttt
//
LOCUS       DAOOYB010000104         9532 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_7831_length_9532_cov_9.450459, whole genome shotgun sequence.
ACCESSION   DAOOYB010000104 DAOOYB010000000
VERSION     DAOOYB010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9532
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7831_length_9532_cov_9.450459"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            31..2184
                     /locus_tag="PL056_07470"
     CDS             31..2184
                     /locus_tag="PL056_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46893.1"
                     /translation="MKKDKIILICVFIFISAFYFISCGNSGWDEILDKECISNSDCPD
                     GYFCDTVTEKCAKSSNNDGSGGLPDNGGLPGDDSSDISDDDNGTAPKGDVKCDCFGTE
                     YTIPGKFENIQGWCKADADGDGIPNCIEVANGIEVDTEEDGTSDYLDTDSDGDSISDS
                     TECTDIPCRDTDSDGVPDYRDDDSDGDALLDNVECFSDPCIDSDSDGTPDYIDTDSDD
                     DGVPDVYETAKDTDKDGMPNFLDDDSDGDGISDSDECPSLPCADSDDDGIYDFLDLDS
                     DGDGLPDNQEVDCPNLGIHSRTKADTDDDGFSDLAEILTGSDPCDSANGVFDTGVKFY
                     FELPYQGEEKDDILTFAPQVKMADIFFNVDTTYSMSGAITNLKSSLSSVIIPGVRNKI
                     TNSAFGVSYFDDFPVSNYGTGSDRPFGLLQSPTTDTATAQAGVDKLALHNGNDVPESG
                     YESLYRIATGVELRWNGGSVPAGNVGFRAGSIPIVVHITDAVAHTTGQTPYSSSYVYT
                     IFSHDQTVTALNSIGAKVVTVHNGSVAAAITQLSNISNGTGAIVPSCANAGRTTLLYQ
                     ISSNGTGLDTSTVNGIDALIKFATFSVYVVPADDGDGGTIDTACFLKKIEAIEYVPVD
                     SCAATITATPGAFNGSSYNNGFSNFSTGTSSATNPGNQLKFKVVAQNDTCFESTGAAK
                     MFKAFINVIDNATGSVLDTQEVTIIVPGKVSGSDA"
     gene            complement(2188..3870)
                     /locus_tag="PL056_07475"
     CDS             complement(2188..3870)
                     /locus_tag="PL056_07475"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPM46894.1"
                     /translation="MPGFKNWYLKWKLLALNFIIIFVFSILTIFTTQYMVSNVSDDIL
                     ESNLSNMLSSITNQIETIRRINAREVQDTQGFNRILREIENGLSGTTIKGDGILAVLD
                     SNTGQWIVHPSAKGQNWSSKHDFIKKMIDSGDGIVHFEMDDSGDKHSAVYRSYKPFNW
                     VIVAIAPVDSASGFLFSFGFNSVIIFILFMIVYAFFTYLISEKMIINPLRNINEAFRT
                     GTKGNLDVMLKKAYNDEIGEMTDNFNEFITTLKNIIFSIRNTAESILNSSNEISGGNN
                     QLSSATQQMASSLEETAASVEEISASIHETAEISVVLTDNITMTAARAEKGKEHLKQM
                     EKAINQVKESGARISEIVDMVNSIAFHTNLLALNAAVEAARAGEQGKGFAVVASEIRT
                     LAQRSADAASEIKQLVDSNEELTTNAGVITKATSQILMDVVTNIQNNASEMRDIEGRS
                     KEQSKGIKQINSAIMQMDEVTQRNAALVEQLASSAMDMAAISKSLNDEVLRFKLNENE
                     VFKPKPLVVTESVTESKRDPFTVGFDEKKSKPFEESDFDSNSFLDDDQFEEF"
     gene            3997..4569
                     /locus_tag="PL056_07480"
     CDS             3997..4569
                     /locus_tag="PL056_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005987774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HPM46895.1"
                     /translation="MKVVAFNGSPRNDGNTAAMIRHIFSVLEPEGIECELIQLGGNIV
                     RGCRACGKCKEMKNGKCVFNDDMMNEWIEKMIEADGIIIGAPTYFASINAETKAFIDR
                     AGYVSRANGNLLRRKTGVAVVAARRAGFLNVFQAINNFFLVMEMIVPGSIYWNLSVAR
                     DKGDFEKDEEGVRTMEALGVNMAWLLKKLN"
     gene            complement(4575..7562)
                     /locus_tag="PL056_07485"
     CDS             complement(4575..7562)
                     /locus_tag="PL056_07485"
                     /inference="COORDINATES: protein motif:HMM:TIGR01451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46896.1"
                     /translation="MYRKLFVVFIFIALAVPFYAAAKAIGPEVSEYRGQELKWEDGAY
                     GYHVMFKSLLENLQADQGNPQADACKNSSTYTLDPAHVPLDALVSQAFLIWAGAQPIA
                     KANDTTDSEVTLSYSSTENGISETQVIKGKKAYKISEAAGFEFDAFKDIDNPNHSYFT
                     YRVDVTEFFKNIHDKGRELGLEYDGASLYGNYTLSDLECASDSSYIGSTEMVSGWTLV
                     IIYSSIEISPKKIYMYDGFKAYFHELSEIGVTGFEFPTDPEVRITLVTHEGDPNLAVL
                     ENPNGGPAIPEGLQVQGDQVGWLLLWNECNPEAFETNGMTTLYYTEIYNSISSVYGWA
                     DVDPTCVGGVPPVWDYEAIEYAIDADTFVMDSASDGSYASHFNKGGQKINLRIGANQD
                     QIITNFMLVSVDTKAPKFDIPGQPEKVACTPANIPVEPGNPESKWCEGNVEHTFAIRV
                     QNWGDDITPSVIVKDTIPTNMEYVPGSTEYATEFKVDNNKQIAKKWMKIPDNGGFPLE
                     SGFKVADKMDFCDAGSDYLSCSDLIMVRFKAKVKSGTPKHSILENTASIDSAGLSSYK
                     TNLGMPVKLRLETSGCVSNADDVNLDDCGGIGVPGCEKDEDCAEGFKCDTEENICVDN
                     QEKCENSDITVSVGKNSPVSDIIFIAPQENLVVGQIEVTNASGKECYFNLASLKLRID
                     LSENNIVLKNIKLVVDVNGDGIVDSSDRLISSVEALKDNYADFASSDPANRLMGNVKN
                     NLLFVLDAGYKDGEQITRNSTFTPSIESDGIKITDSGKPTITGLPLDFSKFQFEPDDA
                     FIVTKGPNDPAVPAKNEMNKTLDILQLKVVSKSSDDKIKSIFVKIPKATMAKFGSAIN
                     NLSIYEDTDNDGKGDTKLITASSTDSTTSHEFKVEIPFTTDVPKYLTVRASLNLKDGD
                     SFQIQIWKVSIDSDKDKLGLPVNSKEYSYTCDPVYEDCGGDDGGCSISSISTAGMNIT
                     EIISAIALFVSAFLVFFRRSFGK"
     gene            complement(7657..8895)
                     /locus_tag="PL056_07490"
     CDS             complement(7657..8895)
                     /locus_tag="PL056_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46897.1"
                     /translation="MKRFFSASVFILFPFFVFSQAWECTDADDCSAGMTCHEELCIAP
                     SANFSTYAAVTLKTGDNTPNSLGSKKIFVAHPAKDVVLGQLGIDSYAGGAEGKLYLFK
                     ELTVTVVSSSLDIKGTNFRLVYDNNGNGLFDSSEKVIATVESPEGSTVKFQIGQKTAS
                     YKMNVTENFLVVGDFSIDKELTSLWDFGVDIKPSTGISISTAGGTGSVEIAAYPEKIS
                     FPRFSFEPESGYFLFASGKYFPKAPSWKEMNKTQTIMHIRTKALDGENEIKSINIRLD
                     GTIVSFGNGVKKISLSLDNGNKGKADSLIEEKTDFVTPVQNVQFQMPSGTLSMEEGEE
                     KFLVVTAELDFYNGQTTQFYITANDVILSKNQQIAGATVITEQFKYSCDETDSECKKA
                     PGNEPEEEDESTGCTVIYVD"
     gene            9081..>9532
                     /locus_tag="PL056_07495"
     CDS             9081..>9532
                     /locus_tag="PL056_07495"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPM46898.1"
                     /translation="MVRPLRITYPGAIYHIINRGCRKDKIFKRIADREEFLARLTAAS
                     SKYGIIIHGYCLMDNHFHLLVETPDENLSAFMQLLQCGYANWFKVRHKLVGPLFQSRF
                     KSVLVEDESYLVTLSAYIHLNPVRAKMTNDPESYRWSSCGQYLRSEKT"
BASE COUNT         2679 a         1924 c         2008 g         2921 t
ORIGIN      
        1 tggtgtgttg attttttact ggaggaaaag atgaaaaagg acaaaataat tttaatttgt
       61 gtttttatat tcatatctgc attctatttc ataagttgcg gcaattccgg ctgggacgag
      121 atactagata aggaatgcat ttcaaattct gactgcccgg acggctattt ctgtgatact
      181 gttacagaaa aatgtgcaaa atcttcaaat aatgacggga gtggcggtct gccggataat
      241 ggaggacttc cgggagatga ttcgtcagac ataagtgacg atgacaatgg aacggctccc
      301 aaaggtgatg tgaaatgtga ctgtttcgga acagagtata cgattcctgg aaaatttgaa
      361 aatattcagg gctggtgcaa agccgatgct gacggagacg ggattccgaa ctgcattgaa
      421 gttgcaaacg ggatagaagt tgacacagag gaagacggca catcagacta tcttgatacg
      481 gatagcgatg gcgacagtat ttccgacagc acagaatgta cggacattcc ttgcagagat
      541 acagattcgg atggagttcc tgattacaga gatgacgaca gtgatggcga tgcactgctt
      601 gacaatgttg agtgtttctc ggatccatgc atagattctg atagtgacgg aacacctgac
      661 tatattgaca ccgacagtga cgatgacggt gtccccgatg tttatgaaac tgcgaaggat
      721 acggataagg atggtatgcc caatttcctt gacgatgatt cagacggaga cggcatatct
      781 gacagcgatg agtgcccgtc acttccatgt gcagattcgg atgatgatgg aatatatgac
      841 tttcttgatc ttgattctga tggtgacgga ctccctgaca atcaggaagt agactgcccc
      901 aatctcggaa tacacagcag aacaaaggcg gacaccgatg atgacggatt cagtgatctt
      961 gctgagatat taaccggctc agatccatgt gacagcgcaa acggagtttt tgacacaggt
     1021 gtcaaatttt attttgaact tccgtatcag ggtgaagaaa aggatgatat tctaactttt
     1081 gcacctcagg tcaaaatggc cgatattttt tttaatgtgg atactacata ctcgatgagc
     1141 ggagcaataa ccaatctcaa atcatcgctg agttcagtta taattcccgg agttaggaat
     1201 aaaattacaa acagtgcatt cggagtatcg tattttgatg attttcccgt aagtaattac
     1261 ggaacaggaa gtgatcgtcc atttggattg ttacagtcac caacaaccga tactgcaaca
     1321 gcgcaggcgg gtgtcgacaa gcttgcactt cataatggaa atgatgtccc tgaatcagga
     1381 tatgaatctc tttacagaat tgctacaggg gttgagctta gatggaacgg aggaagtgtg
     1441 cctgccggaa atgtaggttt cagagcaggt tccattccta ttgttgttca tataacagac
     1501 gctgttgcac atactacggg ccagactcca tacagttcat cgtatgttta cactatcttt
     1561 tctcatgatc aaactgtaac agctttaaat tcaataggcg caaaagttgt tactgtacat
     1621 aacggaagtg tggcagcggc aataacacag ttgtcaaata tatcaaacgg tactggtgcg
     1681 atagtgccgt cttgtgcaaa tgccggtcga accacacttc tctatcagat atcttcaaat
     1741 ggtaccggtc tggacacttc aaccgtcaac ggcatagatg ccctgataaa atttgcaaca
     1801 ttttcagtat atgtagttcc tgctgatgac ggggacggcg gaacgatcga cacagcatgt
     1861 tttttaaaga aaattgaggc aatagagtat gtgcctgtcg attcatgtgc cgctactatc
     1921 acagcaactc ccggtgcgtt caacggatca tcttataaca acgggttcag taacttctca
     1981 accgggacat caagtgcgac aaatccgggc aatcagctca agttcaaagt tgttgcacag
     2041 aacgacactt gttttgaatc cacaggcgcg gcaaagatgt tcaaggcatt tataaatgtc
     2101 attgataatg caaccggttc tgttcttgat actcaggaag tgacaataat agttcccgga
     2161 aaggtaagcg gaagcgatgc gtagagatca gaattcttca aactgatcgt catcaagaaa
     2221 tgaatttgaa tcaaaatcac tttcttcaaa tggttttgac tttttttcgt cgaaaccgac
     2281 agtaaaagga tccctttttg attcagtaac tgattcagta acaactaacg gtttcggttt
     2341 aaagacctca ttttcgttaa gtttgaaccg gagaacttca tcgttcagac tttttgatat
     2401 agccgccata tccattgcac tgcttgccaa ctgttcgaca agtgccgcat ttctctgagt
     2461 tacttcatcc atctgcatta tcgctgaatt tatctgctta atgcctttag actgttcctt
     2521 ggaccttcct tcgatatctc tcatttctga agcattattc tgtatgttgg taacgacatc
     2581 catcaatatc tgtgaagtcg cttttgtaat tacaccggca ttggtagtga gctcttcatt
     2641 tgaatctaca agctgtttta tctcgcttgc cgcatcggca cttctttgag cgagagtacg
     2701 gatctcgctt gcaacgacag caaaaccctt gccctgctca ccagctcttg ccgcctcaac
     2761 cgctgcatta agtgcaagca gattggtgtg aaaagctatc gaattaacca tatcaacgat
     2821 ctcacttatc cttgcacccg attctttaac ctgatttatg gctttttcca tctgtttaag
     2881 gtgttctttt cctttttcag ctcttgccgc tgtcatggtt atgttgtcag taagcacgac
     2941 acttatttcg gctgtttcat gtatagatgc acttatctct tcaacggagg cggctgtttc
     3001 ttcaagagaa gacgccatct gctgagtggc gcttgaaagc tgattattgc ctccggagat
     3061 ctcgtttgaa ctgttgagga tgctttcagc ggtgtttctt atcgaaaaga taatgttttt
     3121 aagtgtagtt atgaattcat tgaaattatc agtcatttcg cctatttcat cgttgtatgc
     3181 ctttttaagc atgacatcaa ggttgccttt tgtgccggtt ctgaaagctt catttatatt
     3241 gcgaagcgga ttaatgatca tcttttcaga tataagatat gtaaaaaaag cgtagactat
     3301 catgaaaagt atgaatatta ttacgctgtt gaatccgaaa gaaaataaaa acccagatgc
     3361 gctgtcgaca ggtgcaatgg cgacgataac ccaattgaac ggcttatatg atctgtacac
     3421 agcagaatgt ttatctcctg aatcatccat ttcaaagtga acgattccat cgcctgaatc
     3481 gatcatcttt tttatgaaat cgtgttttga agaccagttc tgtcctttcg cagagggatg
     3541 aactatccac tgaccggtat tggaatcaag tacagcaagg ataccgtcac cttttattgt
     3601 cgttccgcta agaccgttct cgatttccct taatattctg ttaaatccct gtgtatcctg
     3661 aacttctctt gcatttattc ttcttattgt ttcgatctga tttgtaatgg aactcagcat
     3721 attgcttaaa tttgactcaa gaatgtcatc acttacattt gaaaccatgt actgtgttgt
     3781 aaaaatggta agtatcgaga aaacaaaaat gataatgaaa ttcaaagcaa gcagcttcca
     3841 tttaagatac cagtttttaa atccgggcat gatcttctcc gttttcattg gtacttgcaa
     3901 atgttatagc aatacattca gattttcaag atattttgcc ttttaatttt aaggatataa
     3961 gatttttgag acaaaactgt tgtagggagg attgatatga aagtagtggc ttttaatggt
     4021 agtccgagga atgatggcaa taccgctgca atgatacggc atattttttc tgttcttgaa
     4081 cctgaaggga tagaatgcga attaattcaa cttggcggta acattgtccg tggttgtcgt
     4141 gcttgcggga aatgcaaaga aatgaaaaac gggaaatgtg ttttcaatga tgacatgatg
     4201 aatgagtgga tagaaaaaat gatcgaagct gacggaataa taatcggcgc accgacatat
     4261 tttgcttcca tcaatgctga aacaaaagct ttcattgaca gagccggata tgtttcaaga
     4321 gccaacggaa acttattaag aagaaaaaca ggagtggctg tagtggcagc aagaagagcc
     4381 ggattcctca atgtttttca ggcaataaat aatttttttc ttgtcatgga aatgatcgtt
     4441 ccgggttcaa tttactggaa tctttctgtg gcaagggata aaggtgattt tgaaaaggat
     4501 gaagaaggcg taaggacaat ggaagcgttg ggagtaaata tggcctggct tttaaagaaa
     4561 cttaactgat ctgattattt tccgaaagat cttctgaaga atacaaggaa agcggaaaca
     4621 aaaagtgcaa tagctgaaat gatttcagtt atattcatgc cggcagtaga gatcgatgat
     4681 attgagcaac caccgtcatc accgccgcag tcttcataaa cagggtcaca ggtataggaa
     4741 tattctttgg aatttaccgg cagacccagt ttgtccttat cggaatctat tgatactttc
     4801 caaatctgga tctggaaaga gtctccgtct tttaaattca gagatgctct gacagtcaga
     4861 tatttcggaa catccgtcgt aaaaggtatt tcgactttaa actcgtgtga tgtggtgctg
     4921 tctgttgatg atgctgtgat cagctttgtg tcacctttcc cgtcattgtc agtatcttcg
     4981 tagattgaaa ggttattgat ggcagatccg aattttgcca tagtcgcctt tggaattttt
     5041 acaaaaatag attttatttt atcgtcactg ctctttgaaa caaccttcaa ctgaaggatg
     5101 tcaagagtct tgttcatttc attttttgca ggaacggcag gatcgttagg accttttgta
     5161 actatgaatg catcgtccgg ctcgaactgg aatttgctga aatcaagagg aagtcctgtt
     5221 attgtcggtt ttccgctgtc tgtgatcttt attccgtcac tttcaattga cggggtgaat
     5281 gttgaatttc ttgttatctg ctcaccgtct ttatagccgg catcaagaac aaaaagaaga
     5341 ttgttcttca catttcccat aagcctattt gcaggatcgg atgatgcaaa gtcagcatag
     5401 ttgtctttaa gggcttcgac tgaactgata agtctgtctg aagagtcaac gatgccgtca
     5461 ccgttaacat ctacgacgag tttaatgttt ttgagaacga tattgttttc actaagatca
     5521 attctcagtt ttaatgatgc aagattgaaa tagcactctt ttccggaagc attagtgacc
     5581 tctatctgtc cgactacaag attttcctga ggagcgatga atattatgtc gctcacagga
     5641 ctgtttttgc cgaccgaaac ggttatgtcg ctgttttcgc acttttcctg attgtcaaca
     5701 caaatatttt cttctgtatc gcatttaaat ccttcagcac aatcttcatc tttttcgcaa
     5761 cccggaacac ctattccgcc gcagtcgtca aggtttacat cgtcagcgtt tgaaacgcac
     5821 ccggatgttt caagacggag ctttacaggc atgccgaggt tggtcttata tgatgaaagc
     5881 cctgcagaat cgatgcttgc agtgttttcc agaattgagt gtttgggagt tcctgatttt
     5941 acctttgcct tgaatctcac cattatgagg tcgctgcagg aaagatagtc ggaaccggca
     6001 tcacagaaat ccattttatc tgcaactttg aaacctgatt caagggggaa tccgccgttg
     6061 tccgggattt tcatccattt cttagctatc tgtttgttgt tatcgacttt gaattcagtt
     6121 gcatattcag ttgatcctgg aacatattcc atatttgtag gaattgtgtc tttaactatt
     6181 actgaaggtg taatatcatc tccccagttc tgaactctga tagcaaaagt atgttcaaca
     6241 ttaccttcac accatttgct ctcaggattg ccgggctcaa caggtatgtt tgccggagta
     6301 catgcaacct tctccggctg tccaggaatg tcaaatttag gagcttttgt gtcaacggaa
     6361 acgagcatga agtttgtaat gatctggtcc tggtttgctc cgattctaag gttgattttc
     6421 tgaccgcctt tgttaaaatg tgacgcataa gatccatcag atgctgaatc catgacaaaa
     6481 gtatctgcat caattgcata ttcgattgct tcgtaatccc atacaggcgg aactccgccg
     6541 acacatgtgg gatcaacatc agcccatccg tagacagagg agatggaatt gtaaatttct
     6601 gtataataaa gagttgtcat tccgtttgtt tcaaatgctt ccggattaca ttcattccac
     6661 agaagaagcc atcccacctg atctccctgg acctgaagac cttccggaat tgccggtccg
     6721 ccgttaggat tttcaaggac agcaaggttc gggtcacctt cgtgtgttac aagagttatt
     6781 ctcacttcag gatcggtcgg aaattcaaaa ccggtaacac cgatctcact gagttcatga
     6841 aaatatgcct tgaatccgtc atacatgtaa attttctttg ggcttatttc aattgaagaa
     6901 taaatgatga ccagggtcca gccggaaacc atttcggtag agccgatata gcttgaatca
     6961 gatgcacatt caagatctga gagcgtgtaa tttccgtaaa gtgaagctcc gtcatattca
     7021 agaccgagtt ctcgcccttt atcatgaatg tttttaaaga attcagtcac atcaactcta
     7081 tatgtgaagt aactgtgatt agggttgtcg atgtctttaa aagcatcaaa ttcaaagccg
     7141 gcggcttctg agatcttgta tgcctttttc cctttgataa cctgtgtttc acttattccg
     7201 ttttctgtgg aagaatagct caatgtaact tcactatcgg ttgtgtcatt ggcttttgcg
     7261 atcggctgag cacctgccca gataagaaat gcctgactta caagagcgtc taacggaaca
     7321 tgagccgggt caagcgtgta agtgcttgaa tttttgcagg catctgcctg cggattgcct
     7381 tgatccgcct gaaggttttc aagaagactc ttaaacatga catgataccc gtaggcaccg
     7441 tcttcccatt taagttcctg tcctctgtat tcggaaactt caggaccgat cgcctttgct
     7501 gcggcataaa agggaaccgc aagtgcgata aaaataaata caacaaaaag ttttctgtac
     7561 attttacacc tcaaaaaact gttaaatgcg ataacaacac caactttagt tatgataaac
     7621 aaacatattt atttgtcaaa aattaaaaaa attacatcaa tcaacataaa taacagtaca
     7681 acccgtgctt tcgtcctctt cttcaggttc atttccagga gcttttttac actcactgtc
     7741 tgtttcatca caactgtatt taaactgctc tgtgatgaca gttgcccctg cgatctgctg
     7801 gttttttgaa agtattacat catttgcggt aatatagaac tgggttgtct gtccgttata
     7861 aaaatcaagt tccgctgtaa ctacaagaaa tttttcttca ccttcttcca tggagagcgt
     7921 tcctgaaggc atctggaact ggacattctg gacaggtgtc acaaaatctg ttttttcttc
     7981 gatcagagag tccgctttac ctttgttgcc gttatcaagg gaaagagaga tcttttttac
     8041 gccgttaccg aaagaaacga ttgttccgtc aagccggatg ttgatgcttt ttatctcatt
     8101 ttctccgtca agagctttag ttctgatgtg cattatggtc tgggttttat tcatttcttt
     8161 ccatgacggt gctttcggaa agtattttcc cgaagcgaaa aggaagtacc ctgactcagg
     8221 ttcaaaagag aatctcggga atgaaatttt ttcaggatat gctgctattt caactgaccc
     8281 tgtaccccct gcagtagata ttgaaatccc tgttgacggt ttgatgtcaa cgccgaaatc
     8341 ccacagtgat gtcagctcct tatcaattga gaaatcacca acgacaagga agttttctgt
     8401 gacattcatc ttgtatgagg cagttttctg tccgatctgg aatttaactg tgctcccttc
     8461 gggtgattca acagttgcaa taactttttc cgaactgtca aataaaccgt ttccgttatt
     8521 gtcgtataca aggcggaagt ttgttccttt tatatcaaga cttgaactga ccacagtaac
     8581 tgtcaactcc ttgaaaagat aaagttttcc ttcagcacca ccagcgtatg aatctatacc
     8641 gagctgaccg agaacgacat cttttgccgg atgtgccacg aatatttttt tagatcccag
     8701 tgaattcggt gtgttatctc ctgttttaag tgtcactgcc gcataagttg agaaatttgc
     8761 agaaggagct atgcaaagct cttcatggca ggtcattccc gctgaacagt catctgcgtc
     8821 agtacattcc catgcctgcg aaaaaacaaa aaaaggaaat aaaataaaaa ctgatgcact
     8881 aaaaaatctc ttcatttttt aaatctccaa gttattacaa cacagcaatc ggccgatact
     8941 acttatgacc accgtttttg tcaaaaaaag attccttatg aatggcacat tttttagatt
     9001 aagggggatt aaggggtcag gtcgccataa ctacacatct tttttgttga cattataaaa
     9061 atctagtgta atattcactc atggttagac ctttgcgaat cacatatccg ggtgctattt
     9121 accacataat caaccgtggt tgcagaaaag ataagatatt taagcgtatc gcagaccgcg
     9181 aggaatttct tgcgagatta actgccgcat catcaaaata cggaataatt atccacggtt
     9241 actgccttat ggacaatcat ttccatctgc ttgtagaaac tccggatgag aacctttctg
     9301 ctttcatgca gctgcttcaa tgcggttatg ccaactggtt caaggtgagg cacaaacttg
     9361 tcggaccgct tttccagagc cgttttaaat ctgttcttgt cgaggatgaa agctatcttg
     9421 taacactttc cgcttatatt catctaaacc ctgtcagagc taaaatgaca aatgatcctg
     9481 agagttacag atggagcagt tgcggacaat atttgaggag tgagaaaaca ga
//
LOCUS       DAOOYB010000105         9411 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_7972_length_9411_cov_12.781851, whole genome shotgun sequence.
ACCESSION   DAOOYB010000105 DAOOYB010000000
VERSION     DAOOYB010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9411
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7972_length_9411_cov_12.781851"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            9..884
                     /locus_tag="PL056_07500"
     CDS             9..884
                     /locus_tag="PL056_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013407033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HPM46899.1"
                     /translation="MAENELIQIDDIKSRIFTIRGMQVMLDSDLAEFYQTETKYINRA
                     VKRNPDRFPEGFIFQLTKTEMESLRFQFGTLKTGRGQHRKFNPHVFTEAGVAMLSAVL
                     QTEISIRVSVQIIQAFVKIRHFILENASIFQRLSTVEVKQIEADKKFDMLFKAMEGDQ
                     LPSKQGVLFDGQIFEAHKFVSDIVRSAEKSIILIDNYVDDTVLTLFSKRKQGVVLKIL
                     TKNLSKQLILDTQKFSSQFPPAEIIEFNHSHDRFMIIDDKDLYHFGASLKDLGKKWFG
                     FSKMDVEVTKMLSRI"
     gene            complement(899..1465)
                     /locus_tag="PL056_07505"
     CDS             complement(899..1465)
                     /locus_tag="PL056_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HPM46900.1"
                     /translation="MENMNIYITGVGGQGIGMLSEILVRSVSYAGYKFKAVDTHGLAQ
                     RGGTVVSQLRIGTEVFSPLIESGDADLVISMERHEALRAMTSMLKNGGTIVWYDTSWQ
                     PLPVRTGTDKEVQAEEIANEAKAKNIKNMRVYIEDLPDARMQNITLLANILKNKVIPG
                     LELDHCEKAMKELMSEKVFVKNIEMLSK"
     gene            complement(1476..3335)
                     /locus_tag="PL056_07510"
     CDS             complement(1476..3335)
                     /locus_tag="PL056_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007935019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPM46901.1"
                     /translation="MINYGEMLLKNESFSEIVIGNTAIVRAMIESGVRVVTSYPGSPT
                     PEIAVAIATIPHELRPFYFEFSTNEKVATEVAYGASVNGHLSCVFFKSVGLNVAADTF
                     VQLGHMNLTGGMVVVLGDDPGAHSSQNEQDNRHIAKLSYTPVLEPSSPKEVYEYFKAA
                     AALSVEKKMPVILRLTTHTCHQKELVDFAGWEPQPFDKTPRFDRLNGPYIPITSAVFP
                     MKRRALARLKDVAADKQIKKLNTFINNKNRTGIITAGTTFLSVMDVLKDSDKLPDILK
                     LALVYPLQMDEIVDFLNSHDEVKILEELDDIIEKEIKSIAYERNLKCRITGKTDPEDF
                     IGEYTPDKVRQILSRDFPQMVKSSDFVIIGKTAPIRPPQMCPGCGHRSAFYAIKKALK
                     ETDIKVGDIGCHTLGFLPPYNMVELLMSMGASTGIASGMALFNNSRKVVCFLGDSTFF
                     HAGAAGIVNAIFNKHNITLILMENGTTAMTGHQEHPATGRNFDEITDRIPVRQMLEGM
                     GVAKIFEVDTYHQKKLTEAVAEAVNYEGFSVVIAKHPCMLKFTKAQKRKGSDSGKQVL
                     IDQQKCDRIQECIKNFACPTFNKNGDGSITVNKDLCIGDGSCIQTCPATAIKY"
     gene            3495..4370
                     /locus_tag="PL056_07515"
     CDS             3495..4370
                     /locus_tag="PL056_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46902.1"
                     /translation="MNSIQRFSCLVILLIMAGCGSAQSTSSGNDVMLRSSAASRPQWV
                     SSAGTVKSGGNTMFVGRASGVRNETDAISIATNDAFTKMSNHFGVSVKSDFISSEQET
                     DGQYSYQIGLESRITGHQIKVKNYNLEATYTEQWQRSSKEFDAYVLLAVPDNEIARIR
                     IEVEGAGAWSVISNHEEAVVKTKDLLNVLAKKRKIKFNQQPVPLTPDYDVKDVSGKVE
                     VAFLLVVKCDIGKTEEYSGEFYTEVKTVVELVSLIEGKVLDRWNLETKGGAFSKQDSI
                     NNGIQQSFKNILDKL"
     gene            4386..5324
                     /locus_tag="PL056_07520"
     CDS             4386..5324
                     /locus_tag="PL056_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46903.1"
                     /translation="MNFLKISLIFVVAAVFLNLSAQTEKELQEIKEQMEKMPVPPVVT
                     LPENYEELVKSSSGNVMEKRINIIVGEAITKGKVVLEKVNDILFAQVVPNFAYADDSG
                     EERLGEDLIAILQSNKMEAPRSAQFMNLDYRELMLMKLPFKKRLVDSMVIGADKFDLS
                     LDLTFFVNMNALTNSVYNALRNIKEFTTVYNSSEKYYRTKFDDSNLLFLVRIPNVAYE
                     LVYTGELFENIISAQIKEALFLKNVIKDFEKQIEKDKELKFDGRYIKGKDISIDLWFL
                     AVETEMNVEKGKDFIRKALKHESVNVTGSEKKKDRK"
     gene            5321..6019
                     /locus_tag="PL056_07525"
     CDS             5321..6019
                     /locus_tag="PL056_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46904.1"
                     /translation="MISCPHCGFENNNYFFCAGCGRGIPVKKKSVFTKLPESSTRFLE
                     EAVNTIERVFYTDNSIHTTLDMNTTLINMSEISKESGIFIIDDAQWKDVEGKVKLSCF
                     FGETALAYILSFVLSLIGVMLGSAGIEMTFKLFFTCYILVSFLQWFIFPYIAGSTVVA
                     FAGHRCGLFVDRKFSVRGRPGLLLILSLFMLLHSFIPFFLIEYLLAAKIKSYVPVALK
                     ITGIDYLKKIGGDQ"
     gene            6016..6960
                     /locus_tag="PL056_07530"
     CDS             6016..6960
                     /locus_tag="PL056_07530"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPM46905.1"
                     /translation="MKQFVKTAILTAVLVLFSSCGINKEVHQKALNDLASAQKNHKDE
                     QQVNEKLKKEIETLKKENEGLKKDLLEVINEKMGLNREIADLKQRNIDSANMVETLMF
                     QLKEKGASLEDLEAKKGEMDEQREKMLAERDRLLKEQDNLKGELEQVRKMKEAAEKRN
                     AEFQKLMSKLKSMIDAGKLSVTIRKGRMIVSLSSDILFPSGKTTLTDEGKLAIAELSE
                     TLKTLEDRSFLIVGHSDSTPIKTKRFPSNWELSSQRAIEVARLMVESGVKPEMLTAAG
                     QAEFDAIAENDTPENKAKNRRVEIILLPKIEELPGFPQ"
     gene            complement(7036..7296)
                     /locus_tag="PL056_07535"
     CDS             complement(7036..7296)
                     /locus_tag="PL056_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46906.1"
                     /translation="MRHFISENLKVSQKFEIIEKKQIKYQLESDKKFDILFKALENNE
                     LHPKQGILFEGQVFEAHKFVSDIVRFGFSKMNIEVTKMLARI"
     gene            complement(7301..7366)
                     /locus_tag="PL056_07540"
     CDS             complement(7301..7366)
                     /locus_tag="PL056_07540"
                     /inference="COORDINATES: protein motif:HMM:NF022018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46907.1"
                     /translation="MLDSDLAELYGVETKALNPGG"
     gene            7607..7984
                     /locus_tag="PL056_07545"
     CDS             7607..7984
                     /locus_tag="PL056_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46908.1"
                     /translation="MPKYNYEDFRKACGRKKGVDVVVFDTAQNDAGEFFSLYTKKELM
                     SFIFNDGFENITHFSSPKHWHLPDIFIDEYEFRILGKTGCLAFFYFNGKWVVKSFHLS
                     KTRFSIMADNIIKILEKRKKQND"
     gene            7977..8552
                     /locus_tag="PL056_07550"
     CDS             7977..8552
                     /locus_tag="PL056_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46909.1"
                     /translation="MTEKKCPVCGSNEIYLNIKKRKITDPFGGEREINVNVYSCKICG
                     TVGDFFNENDRIISNAISFLNDKSVKNIIDFLTNSGFKLAAVEKALGLSQRTLTKWKI
                     GATRPSSSSVALLKLIKLFPWLLDVADSKYDETVATRIFIEEFFVYFSNYDKLLTAVT
                     EDTCLLMGKKSNKSDISVVCGYGSDPLIEIQ"
     gene            8569..9003
                     /locus_tag="PL056_07555"
     CDS             8569..9003
                     /locus_tag="PL056_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46910.1"
                     /translation="MKIRTFIICDDIRSEIGGKHSLIGVYKDRIIFPSSMEQKNNWPK
                     AIKIGFYVEVEIDKKLPASFVFKRIESNDESEILKGDINISKDSKIIRMTLLHPAFIF
                     KKSGVMRFIFEFYDKTGKKICTVSPEIDFNIEEMSSEQIKKL"
     gene            9049..>9411
                     /locus_tag="PL056_07560"
     CDS             9049..>9411
                     /locus_tag="PL056_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46911.1"
                     /translation="MIKLLFLILAITTLYSCGDNDNDIEWSERSNTKLNLSDAYDYCN
                     GLGGRIPSISELRELVVNCAPTETKGECGIKDECSLSESCLNNTCSGCEESSLGIYSS
                     FKDIGPYWSSSFFQNSNVW"
BASE COUNT         2924 a         1705 c         1935 g         2847 t
ORIGIN      
        1 gtcggaaaat ggcagaaaat gagttgatac agattgatga tattaagagc agaatcttca
       61 ctattcgcgg aatgcaggtg atgctggaca gtgatcttgc ggagttttat caaacagaaa
      121 caaaatatat aaacagagca gtgaaaagaa atcccgacag attcccggaa ggatttattt
      181 ttcagctcac aaaaactgag atggagtctt taaggttcca atttggcacc ttaaagacag
      241 gcagaggaca gcatcgaaaa ttcaaccccc atgtttttac tgaagccggt gttgcaatgc
      301 tgtcagcagt tctccagacc gaaatttcaa ttcgtgtaag tgttcaaatt attcaggcat
      361 ttgttaaaat acgacatttc attttggaaa atgcatcaat atttcagcga cttagcactg
      421 ttgaagttaa gcaaatagaa gctgacaaaa aatttgatat gctttttaaa gccatggaag
      481 gcgatcagct tccttcaaaa cagggcgttt tatttgacgg acaaatattt gaagcacaca
      541 aatttgtttc agatattgta aggtcggctg aaaaatcgat cattcttatt gataattatg
      601 ttgatgatac agtcctcaca cttttttcaa aacggaaaca gggagttgtg ctcaaaatcc
      661 tcacaaaaaa cctttcaaaa cagttaattc tcgacactca gaaattcagt tcacagttcc
      721 ctcctgctga aataattgaa ttcaatcatt cccatgacag attcatgata attgacgata
      781 aagaccttta tcattttggt gcatcactca aagatctcgg caagaaatgg tttggattct
      841 ccaaaatgga tgttgaagtg acgaagatgt tgtcaaggat ataacttttg ctaccttgtt
      901 actttgataa catctcaata ttctttacaa aaaccttttc actcatgagt tctttcattg
      961 ctttttcaca gtgatcaagt tcaagtccgg ggataacttt gtttttgagg atatttgcta
     1021 aaagggtgat gttctgcatt cttgcatcgg ggagatcttc gatgtaaacc ctcatatttt
     1081 ttatgttttt tgcttttgct tcatttgcaa tctcttcagc ttgaacttct ttgtcagttc
     1141 ctgttctaac cggcaggggt tgccacgagg tgtcatacca gacgattgtt ccgccgtttt
     1201 tcagcattga tgtcattgcc cttaacgctt cgtgccgctc catgcttatg acaagatctg
     1261 catcccccga ttcgatgagc ggagagaaaa cttctgtacc gatcctcagt tgactaacga
     1321 ccgttccgcc tctttgcgca agtccgtgag tgtcaacggc tttgaattta tatcctgcgt
     1381 aactgaccga tcttactaat atttcactca acatcccgat tccctgaccg ccaactccag
     1441 tgatgtagat attcatgttt tccataattt ttctcctaat attttattgc tgtcgcagga
     1501 caggtctgga tacagcttcc atcgccgatg cacagatctt tgttgacagt aatgcttccg
     1561 tcaccgtttt tattgaatgt gggacatgca aaatttttaa tgcactcctg gatccggtca
     1621 catttttgct gatcgatcaa aacctgtttt cctgaatcgc ttccttttct tttctgtgcc
     1681 ttggtgaatt tgagcatgca gggatgtttt gcaataacta ctgaaaaccc ttcataattt
     1741 acagcttctg ccacagcttc tgtcaacttt ttctggtggt atgtgtcaac ttcgaaaatt
     1801 tttgcaaccc ccattccttc aagcatctgt ctgactggaa ttctgtcagt tatctcatca
     1861 aaattccttc ctgtcgcagg atgttcctga tgcccagtca ttgcggtagt tccattttcc
     1921 atgaggatca gagtgatgtt atgtttattg aaaatcgcat tgacgatgcc cgccgctcct
     1981 gcatggaaaa atgttgaatc tccaagaaaa cagacaactt ttcttgagtt gttgaaaagt
     2041 gccattccgc ttgctattcc tgttgaagcc cccatgctca tcaaaagctc gaccatattg
     2101 taggggggaa gaaatccgag agtatgacag ccgatgtctc cgacttttat atcagtttcc
     2161 ttcaaagctt tctttatcgc ataaaaagcc gatctgtgac cgcatcccgg gcacatctgg
     2221 ggaggtctga tcggtgcggt tttgccaatt attacaaaat ctgagctttt aaccatttga
     2281 ggaaaatcac ggcttaaaat ctgccgcact ttgtcgggag tatattcgcc gatgaaatct
     2341 tcagggtctg tttttccagt aattctacac ttcaggtttc tttcgtaagc aattgatttt
     2401 atttcttttt caatgatatc atcaagttct tcaagaattt tcacctcgtc gtgactgttt
     2461 aaaaaatcaa cgatctcatc catctggagc ggatagacca gggcaagttt taaaatgtca
     2521 ggcagtttgt cagaatcttt aagaacatcc atcaccgaaa gaaaagtcgt tcccgctgtt
     2581 attattccgg ttctgttttt attgttgatg aaagtattca gttttttaat ctgtttatct
     2641 gccgcaacat cctttaatct tgcaagtgcc cgtcttttca ttgggaaaac tgcggaagtg
     2701 atcggaatgt atgggccgtt aagtctgtca aatctcggcg ttttatcaaa cggttgcggt
     2761 tcccatccgg caaaatcaac aagctccttc tgatgacagg tatgagttgt gagccgcaat
     2821 atcacaggca ttttcttttc aaccgataat gccgccgccg ccttgaaata ttcataaact
     2881 tctttcggag atgacggttc aaggacagga gtgtaagaaa gttttgcaat atgtctgttg
     2941 tcctgttcgt tctgtgaaga atgcgctccc ggatcatcac cgagaacaac gaccattccg
     3001 ccggtcaggt tcatgtgccc aagctgtaca aatgtgtcag cggcaacatt aagtccgacg
     3061 cttttaaaga aaacacagct taagtgcccg tttacactgg ctccgtatgc aacttcggtc
     3121 gcaacctttt cgttggttga aaattcaaaa taaaaaggtc tcaattcatg cggaattgtc
     3181 gcaatggcaa ccgcgatctc aggagtagga ctccccggat aacttgtcac aacgcggacg
     3241 ccagattcta tcattgccct tacaatggca gtatttccaa tgacgatctc agaaaacgac
     3301 tcatttttta acagcatttc accataattg atcatgatcc ctccggaatt aagaaaaaac
     3361 ttgcggatat taccattttc tgaagattgc gtcaacaatt aagatatttg tgtccttaat
     3421 taacggcata cggtttcttg aattccgcaa agtaatctga tattatctcc cactaactgt
     3481 tttttgagga caaaatgaac agtattcaaa gatttagctg tcttgttatt ctattaatta
     3541 tggctggttg cggttctgca cagagcactt catcaggaaa tgatgtaatg cttagaagtt
     3601 cagctgcttc aagacctcag tgggtaagtt ctgcaggaac cgtaaaaagc ggcggaaaca
     3661 cgatgtttgt cggcagagct tccggagtga gaaacgaaac ggatgcaatt tcaattgcaa
     3721 ccaacgatgc ttttacaaaa atgtccaacc atttcggtgt gtcggtaaaa agcgacttta
     3781 tctcttccga gcaggaaacg gatggtcagt attcatatca gatagggctt gaaagcagga
     3841 tcaccggaca tcagataaag gtgaaaaact ataacctcga agccacttat accgagcagt
     3901 ggcagcggag ttcaaaggag tttgatgcgt atgtccttct tgccgttcct gataatgaaa
     3961 ttgcaagaat aaggatcgaa gttgaaggtg ccggtgcctg gtcagttatt tcaaatcatg
     4021 aagaagctgt tgtaaaaacg aaggacctgc tgaatgttct ggcaaaaaaa agaaaaataa
     4081 aatttaacca gcagccggtt ccgcttactc ctgactatga tgttaaagat gtttccggca
     4141 aggtagaagt ggcttttctt cttgttgtga agtgcgatat aggaaaaacc gaagaataca
     4201 gcggtgaatt ctataccgaa gttaagactg ttgttgagct ggtctctttg atcgagggaa
     4261 aggttttaga cagatggaat ctcgaaacaa aagggggagc gttcagcaag caggattcaa
     4321 taaataacgg tattcagcaa tcgttcaaaa acatacttga taaactctga agatgaggta
     4381 agtcattgaa ttttcttaaa atatcactga tctttgttgt ggcggcagtt tttctgaacc
     4441 tttcagcgca gacagagaaa gagcttcagg aaataaagga acagatggaa aaaatgccgg
     4501 ttccgcctgt agtcacactg cctgaaaatt atgaagagct tgtgaaaagt tcttcaggga
     4561 atgtgatgga aaaaaggata aacatcattg taggagaggc gataacgaaa ggcaaggtcg
     4621 ttcttgaaaa agttaatgac attctctttg cacaggtcgt tcccaatttt gcctacgcag
     4681 atgacagcgg tgaagagcgg ctcggtgagg accttatcgc catactccag tcaaacaaga
     4741 tggaagctcc aagaagtgcc cagttcatga accttgatta ccgagaactg atgctgatga
     4801 aacttccttt caaaaagcgg cttgttgact caatggtaat aggagctgac aagttcgatc
     4861 tttcactgga ccttactttc tttgtcaata tgaacgctct taccaacagt gtttacaatg
     4921 ctctcaggaa cattaaggaa tttacaacgg tttataacag ttcggagaaa tattaccgga
     4981 ctaaattcga tgacagcaat ctcctttttc ttgtgaggat cccgaatgtt gcctatgaac
     5041 ttgtctatac aggggagctt tttgaaaata ttatttctgc ccagatcaaa gaggcccttt
     5101 tcctgaaaaa tgtcattaaa gactttgaaa agcagataga aaaggataaa gaacttaaat
     5161 ttgatggaag gtatataaaa ggtaaggata tttcaataga tctgtggttt ctggcagttg
     5221 aaacagagat gaatgtcgaa aaaggaaaag atttcatcag gaaggcctta aagcatgaat
     5281 ctgtaaatgt aacaggcagt gaaaagaaaa aggacaggaa atgatcagtt gtccccattg
     5341 cggttttgaa aacaacaact attttttctg tgcaggatgc ggaaggggta tccctgtaaa
     5401 gaagaaaagt gttttcacga aacttcctga aagttcaact agatttcttg aagaagctgt
     5461 taatacaata gaaagggttt tttataccga caacagcatt cataccacgc ttgacatgaa
     5521 cacaacactc attaacatgt ctgaaatttc caaggagagc gggatattca taattgatga
     5581 tgcacagtgg aaagatgtcg aaggaaaagt aaagttgagc tgctttttcg gtgagacggc
     5641 tctggcatat attctgagct ttgttctttc tcttattgga gttatgctcg gttctgcagg
     5701 gattgagatg acattcaagc tttttttcac ctgctatatt cttgtttcgt tccttcagtg
     5761 gtttattttt ccttatattg ccggttccac tgttgttgct tttgccggtc atagatgtgg
     5821 tcttttcgtt gacagaaaat tttcggtaag aggtcgtccc gggctgctcc tgatcctctc
     5881 tctctttatg cttcttcatt cattcatccc tttctttctg attgaatatc ttcttgccgc
     5941 aaaaataaaa agctatgttc cggtggcttt aaaaataacc ggaattgatt atttaaaaaa
     6001 aatcggaggt gaccagtgaa acaatttgtg aaaaccgcta ttttaactgc agttcttgtt
     6061 cttttctctt catgcggaat taacaaggag gttcatcaga aggcattgaa cgatcttgca
     6121 tctgctcaga agaaccacaa ggatgaacag caagtcaacg aaaagttgaa aaaagagata
     6181 gaaactctga aaaaagaaaa tgagggactt aaaaaagatc ttcttgaagt gataaatgaa
     6241 aaaatggggc tgaatcgtga aattgctgac ctcaaacaga ggaacattga ttcagcaaat
     6301 atggtggaaa ctcttatgtt ccagttaaag gaaaaagggg catcacttga agaccttgaa
     6361 gcaaaaaaag gggaaatgga cgagcagaga gaaaaaatgc ttgctgaaag agacagattg
     6421 ctgaaagagc aggataatct caaaggcgaa cttgagcagg taagaaagat gaaagaagcg
     6481 gctgaaaaga gaaacgccga gtttcagaaa cttatgagta agcttaaatc aatgatagat
     6541 gccggaaagc tttcagtgac cataagaaaa ggacggatga ttgtttcatt aagcagtgac
     6601 atacttttcc cgtcaggtaa aaccacactt acagatgaag gaaagcttgc aattgctgaa
     6661 ctctcggaaa cacttaaaac acttgaagac agatcgttcc ttattgtagg acacagcgat
     6721 tcaactccga taaaaaccaa aagatttccg tcaaactggg aactttcatc tcaaagggcg
     6781 atagaagttg cacggctcat ggtcgaaagc ggagtaaagc ccgaaatgct cactgcggca
     6841 ggacaggccg aatttgatgc tatcgctgaa aacgatacac cagaaaacaa agcaaagaac
     6901 agaagagtcg aaataatcct tctgcccaag atcgaggaac ttcccggatt tccacaataa
     6961 tcaaagattc ctacctatac ggcttatcac cgcagaattt cataaaattt ctcattatct
     7021 gcgggggtga tttggttata tcctggcaag catttttgtc acttcgatgt tcattttgga
     7081 aaaaccgaac cttacgatat ctgaaacgaa tttgtgtgct tcaaaaacct gaccttcaaa
     7141 aagaattccc tgttttggat ggagttcatt gttttcaagg gctttgaaaa gtatgtcaaa
     7201 ctttttatca gattccaact gatatttaat ttgttttttc tcaataatct caaatttttg
     7261 agaaactttt agattctctg aaatgaaatg tcgcattctt ttaaccgcct gggttcaatg
     7321 ctttagtttc aacaccatac agttcagcaa gatcactgtc cagcatcacc tgcattcctc
     7381 taatcgtgaa gattctgctc ttaatctcat caatctatac cctgtgaatt caaaggaatc
     7441 aaagatctct ttaatcacta catgttaaaa ttgtttttaa taatttgttt tgttgacttg
     7501 ttgttagaaa tatgatagcg tttcatcgtg aagttagtga tcctttaagg atcgaaattt
     7561 gacaaaaaac ttttttgtaa taaattgttt taaagaggct tttttgttgc ctaagtataa
     7621 ttatgaagat tttagaaaag cttgcggaag aaaaaaagga gttgatgtgg ttgtttttga
     7681 cacggcacaa aatgatgcag gtgaattttt cagtctttac acgaaaaagg aacttatgag
     7741 ctttatattt aacgatggtt ttgaaaatat aacccatttc agttctccaa aacactggca
     7801 tctgccggat attttcattg atgaatatga gtttagaatt ttaggaaaaa ccggttgtct
     7861 ggcgttcttt tattttaatg gaaagtgggt tgtgaaatca ttccatctgt caaaaacaag
     7921 attttctatt atggctgata acattataaa gatattagaa aaaaggaaaa aacaaaatga
     7981 ctgagaaaaa atgtcctgtt tgtgggtcaa atgagattta tttaaatatt aaaaaaagaa
     8041 aaattactga cccttttggt ggagaaagag aaattaatgt taatgtttat tcctgcaaaa
     8101 tttgcggaac ggtaggcgat ttctttaatg agaatgaccg aattataagc aatgcgatta
     8161 gttttctgaa tgataaatcc gttaaaaata ttattgattt tctcacaaat agtgggttta
     8221 agcttgcggc tgttgaaaaa gctcttggat tatctcaaag gacattgaca aaatggaaga
     8281 ttggagcaac cagaccttct tcatcgagtg tggctttgtt gaaacttatt aagttgtttc
     8341 cgtggctttt agatgttgcg gacagtaaat atgatgagac tgttgcgacg agaatattta
     8401 ttgaagaatt ttttgtatat ttttccaact atgataaatt gctgacagct gtaactgaag
     8461 acacttgtct tttaatgggt aaaaaaagta ataagtcaga tatatcagtt gtttgcggtt
     8521 atggttctga tccgttaata gaaatacagt aaaaatgggg ttttaaaagt gaaaataaga
     8581 acatttataa tatgtgacga tattcgcagc gaaattggcg gaaaacactc cttgataggg
     8641 gtttataaag acaggataat atttccttcc agcatggaac agaaaaacaa ctggccgaaa
     8701 gcaataaaaa tcggttttta tgtagaagta gaaatcgata agaaactgcc ggcatcattt
     8761 gtgtttaaaa gaattgaaag caatgatgaa agcgagatat taaaaggaga tataaacatt
     8821 agcaaagaca gtaaaataat cagaatgact ctgttacatc cagcgttcat atttaaaaaa
     8881 agcggagtca tgaggtttat attcgagttt tatgacaaga caggaaagaa aatatgcact
     8941 gtttcacccg aaatagattt caatattgag gaaatgtcca gtgaacaaat taaaaagctt
     9001 tgaattgatg aatattttta aaactgtctt tgagctaggg agtttatcat gattaaatta
     9061 ctttttttaa tactcgcaat tacaacatta tatagttgtg gggataatga taatgatata
     9121 gagtggtcag aaagatcaaa cacaaaactc aatttatcag atgcttatga ttattgcaac
     9181 ggtttgggtg gccgaattcc aagtattagc gaacttaggg aactagttgt aaattgtgct
     9241 ccgaccgaaa caaagggcga gtgtggaatt aaagatgagt gctctttgag cgagagttgc
     9301 ttaaataaca catgttcggg ttgtgaagaa tctagcttag gaatttactc gtcttttaaa
     9361 gatatcggac cttattggtc atcaagtttt ttccaaaatt ctaatgtatg g
//
LOCUS       DAOOYB010000106         9356 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_8043_length_9356_cov_12.221589, whole genome shotgun sequence.
ACCESSION   DAOOYB010000106 DAOOYB010000000
VERSION     DAOOYB010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9356
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8043_length_9356_cov_12.221589"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1781)
                     /locus_tag="PL056_07565"
     CDS             complement(<1..1781)
                     /locus_tag="PL056_07565"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HPM46912.1"
                     /translation="MNRFFLLILLLIPFVISAQDPVVTANAKIHPENVRTGEFAKISI
                     TVIPPEGYHLYAMEPVSDGPKPLKISMPLETGTDHLWYGGKPLNEYDPNFKKEVLFYA
                     GPAQFSTALKLSAPLKNSDITVTGQICNEKRCLPFKINLTLNLKFDSGEPRAEFMKIP
                     PFDGIELNKKAQSPFEGGIISLLILAFLAGLGALLTPCVFPMIPITVSFFSKFSKVSM
                     GRALLMASVYAGSIIATFTFIGIAVSAIFGAVGMQALSSSPWFNLFLAVLLIVFAFNL
                     FGYFEISIPSWLITGASKKEQKLSSDEGSLMHQIMGVFLMAVTFTLISFSCTVGFVGI
                     VIAEAAKGNWFNPALGMFSFSLAFSTPFFFLALFPSWADKLKGKGGDWMIAIKAVLGF
                     LELAGAFKFISNVDLVWKWEMISRSFVITIWVGIFVIMALFLLRLFNLPFSDESNKIT
                     GPFRLIGAILAISAAAYLTSVVGTTKSLGGWIDGWLPPIDYLHENTDRTASTDTLNWI
                     KDDIEGAMKKAKADNKPLFIDFTGYTCTNCRFMESGMFPRPVIREELSKMVLLKAYTD
                     GDGEIDQAQRLYQISRFNTAALPFYAI"
     gene            complement(1842..2675)
                     /gene="speE"
                     /locus_tag="PL056_07570"
     CDS             complement(1842..2675)
                     /gene="speE"
                     /locus_tag="PL056_07570"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HPM46913.1"
                     /translation="MWFVENYDEKASLGLKVKRKLHEEQSPFQKIEIYETEFFGNLLT
                     LDGLVMLTEKDEFVYHEMISHMPLCTLKNPERVLVVGGGDGGAIREILKHPSIKEAVL
                     CEIDERVTRLSQKYFPSVSCELENPKVKMHFEDGFKFLDKYENYFDLIITDSSDPVGP
                     GVALFKEDYYRLVKKALKPDGIMVSQSESPWYFEDTMNSMTTAMSSVFKHVETYIALV
                     TLYPSGFWTITAASDAHSLHNFDEKRSAEIAKTCKYYNPELHKGALALPNFVKKIVAN
                     K"
     gene            complement(2675..3169)
                     /locus_tag="PL056_07575"
     CDS             complement(2675..3169)
                     /locus_tag="PL056_07575"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013100750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HPM46914.1"
                     /translation="MIDLKNIPDIHFFCAGASEGRTELTSFDGALLAAGVGNTNLIKM
                     SSILPPKSVEVEPFVIAPGSFVPLAYADITSSEPGETISAAVAIAIPDDRSINGVIME
                     HHNHAPLSYVEAKVREMAAESMEMRGIKNFTIKSKGIEHKVVNIATAFAAVVLWDSKL
                     KGEK"
     gene            complement(3274..7425)
                     /locus_tag="PL056_07580"
     CDS             complement(3274..7425)
                     /locus_tag="PL056_07580"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HPM46915.1"
                     /translation="MDGHFYLGLDLGSVSLNCIITDESGSIIFKRYVRTSGLPVDATL
                     SLFKNIANESGDISFSGAVIAGSGKELIANSTGIKTVNEIVAHATAAWTMYPDVKTVF
                     EIGGQDSKFISIGRKNDGTHYLKDHAFNELCAAGTGAFLDQQAERLGITISELSTLAS
                     RAQNIPSVAGRCSVFAKSDMIHLQQKAVPTDEIAAGLCFALVRNYLATLCKGKIPPAP
                     IIFQGGVAANAGVVKAFREILSLNESEFIVPPNFDVMGALGSSVIAKENMLEEPVSIH
                     ELINKLENRSIIHENEEFREPLSDIFISGDIPNEISNIPLKQPFFLGIDIGSVSTKGA
                     VIDSEKKLVASGYVPTAGKPVEALKNLIEKMISGSKNEISVSNIVSTGSGRHLASIIT
                     GGTTMDEISAQSVSCSHYFSDSDTIIEIGGQDSKFVRIKNGNIETFKMNRACAAGTGS
                     FLEEQAGRLKISIKNEFADLALKSRNPVRLGSRCTVFMDSDLVHHLQRATSTEDLCAG
                     LAYSIGENYLEKVVGSSPVGENIVFQGGVAKNRAVVAVFEKLLNKKISVHPHPEISGA
                     IGAAMTAMTEYEQGISKVLKKLNDLKVEAETETSECRLCENICEIRKITVANGSFAYF
                     GSICGRFEKMSRTHVEFDDMFKIREEIMEKSVIRSKTEPSLGEMAIPDTLTIRDHIPF
                     WVTFLSSIGYSPVLSEKTKRSVVEKGLIHVPAEFCFPMKVLFGHVYNLIDKGYKNIFI
                     PHLRLFTAPNEKTLRYACPYTQAAPYIVRENLTENANILTLEFPVEGEEIHWITETSR
                     KLGLRKNVIEKALKTAFAAQNNFGLQCIEEGRKIIRKLADENRKGAVIIGRPYNTTDR
                     HVNLNLVKKLKDLNIEPIPFDFLPLGEEKLPPIWDRVRWGYGRKLIMAARILKRHKNL
                     GAVIVTNFGCGPDSFIDQYLEYELRDTPHILIEFDDHQAEAGLVTRLEAFSRNFIIND
                     KPVKVITGRDPGKARMPLHEYTYYITSFMDHAYAMTGALKASGCKTVLLPPTDEESWN
                     LGSKYAYGRECHPFISFVGDILKEAQKPGFDPSKACYFGPSYFGACLLPQYIIATHMI
                     LDRIGLGEMIVMNITDATNMKELGPQYMVRLALGTYTIDRFFKWKTEIEPYEATPGEV
                     NRVYRKIIEDLETGLIQGGFFKALRNGVKNLKNVNLTEKDGTKPKIGVVGDVYTRINQ
                     HSNNHLYDRLKDMGFEVWPACSLIDVSFLGMEQLHEELYRKGKKISGFFTKALIPAIK
                     MARWIVDRNFPETIRTPQERDFHAVKKVANRYADLYIDKVLALNLNRIEEFHQSGADG
                     ILNVMCHNCMLGTVTASLTKSIRSDMDDIPLCTLVYEGLKSTHNVNRLEAFAHQVKSA
                     GKDKK"
     gene            7567..>9356
                     /locus_tag="PL056_07585"
     CDS             7567..>9356
                     /locus_tag="PL056_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HPM46916.1"
                     /translation="MDAFQKVLFVDTSNGFYKIKRYKVGDYFGPVDLGIHISDNHRSL
                     NIGTGLFAGSIFPGSNRLIFTGHSPCWGGFFISSMGGSGLVFDNLGINMVSIVGKAQT
                     PSILYLNRNHGEEIEVEVHPVDVSKVWNSGRKGVYSLFDHTLEKFGDRYELDPRILAT
                     GPSSVTTDFGGICSVPIVKKKLTFVDTWAGRGGFGSKMFQEHGIVAIIYGGTTIDEDF
                     RDRTVADEWFEQKYKQKLIAKDFESTTKYRFDPKLGTGGTFGVNFATIKDRIIAFNYK
                     TIFDSDKSRLELTEKFITDHYLLQFNEETIEKKQQATCGEPCPAVCKKMNGEFKKDYE
                     PYQTMGPLCGIFDQRAAEKLNHHCDAMGMDAISGGGVLAWLMELLDNGTLSKEDLGVS
                     KMPKFDLENFDVVKDSQNNADLGIELIDAILNKSGILDLSEGVRKWARKVHRSTGKKI
                     IDSFVFTANGRRGWMVPNQYWTPGALAPMAIMGKYYMYYGNEFLPPRTLGVACADRLK
                     KELILDNAGICRFHRGWAEEMIPEMIESIYDLKEKFLSSVNFTAGRINSRNAAVFLES
                     ERNIEFVYTFLKRKRDVDGETREDLNYWIE"
BASE COUNT         2596 a         1974 c         1999 g         2787 t
ORIGIN      
        1 attatcgcat aaaatggtag agccgcagtg ttaaaacggc ttatctgata gagccgctgt
       61 gcctgatcaa tttcaccatc cccatcggta tatgccttta gaagcaccat ttttgaaagt
      121 tcttcccgga tcacaggtct tgggaacatg ccgctttcca tgaaacggca gttcgtacag
      181 gtgtaccctg tgaaatcgat gaataacggt ttgttatcgg ctttcgcctt tttcatcgcc
      241 ccttctatat cgtcttttat ccagttaagt gtgtcggttg aagctgttct gtcagtgttt
      301 tcatgcagat aatctatcgg aggaagccat ccgtctatcc accctccgag actttttgtt
      361 gttcctacaa ctgaagtgag ataggccgcc gcacttattg caagaattgc cccgatcaat
      421 ctgaaagggc ctgttatttt atttgattca tcactgaaag ggagattgaa aaggcgaagc
      481 agaaatagcg ccatgataac aaaaatgccg acccagatag taatgacaaa agatcttgat
      541 atcatttccc atttccacac aaggtctaca tttgatatga atttgaaagc tccggcaagt
      601 tcaaggaacc cgagcactgc ctttattgcg atcatccagt caccgccttt tccttttaat
      661 ttatccgccc agctcgggaa aagtgcaaga aagaaaaaag gtgttgaaaa agcaagagag
      721 aaactgaaca ttccaagggc aggattgaac cagttccctt ttgccgcctc ggcgatgacg
      781 attcccacga aaccgactgt acagctgaaa cttatcagag tgaatgtaac agccataagg
      841 aagacaccca ttatctggtg catcagcgat ccttcatcag atgaaagttt ctgttccttt
      901 tttgaagctc ctgttatgag ccatgaaggt atcgatattt caaagtatcc gaaaagattg
      961 aatgcaaaaa cgatcagaag tactgcaagg aacaggttga accacggact tgaactgagt
     1021 gcctgcattc cgaccgctcc gaatattgct gaaactgcta tgccgatgaa agtgaaagtg
     1081 gctattatag agccggcata cactgatgcc atgagcagcg cccgtcccat tgaaactttt
     1141 gagaattttg agaaaaatga aactgttata ggtatcatcg ggaaaacaca cggggtgaga
     1201 agtgctccga gccctgcaag aaatgcaaga atcagaagag aaatgatgcc gccttcaaaa
     1261 ggggactgag cttttttatt aagttcaatg ccgtcaaatg gcgggatctt catgaactcc
     1321 gcccttggtt caccggagtc aaattttaaa ttcagcgtaa ggtttatctt aaacggaagg
     1381 cacctttttt cattgcatat ctgaccggtt acggtgatat ctgagttttt taaaggtgct
     1441 gaaagtttca atgcagttga gaattgagca ggaccggcat aaaaaagaac ttctttctta
     1501 aaattaggat catattcatt caaaggtttc ccgccatacc agagatgatc tgtacctgtt
     1561 tccaaaggca ttgatatctt taaaggtttc ggtccgtcag acacaggttc catcgcataa
     1621 agatggtatc cttcaggggg aatgacagta atagatattt tggcaaattc acccgttctg
     1681 acattttcag gatgaatttt tgcatttgca gtgacaacag gatcctgagc agagataaca
     1741 aatggaatta ataaaaggat cagaagaaaa aaacggttca tcggacacct cataaaaaat
     1801 catatcggaa gcattttatc agaatttatt cagatgacaa attacttgtt ggcaacgatc
     1861 tttttaacaa agttggggag ggcgagggcc cctttatgga gttcggggtt ataatatttg
     1921 caggtctttg cgatttctgc ggatcttttt tcatcaaaat tgtggagaga atgtgcgtcg
     1981 cttgcggcgg tgattgtcca gaatccgctt ggatagagtg taacaagtgc aatatatgtt
     2041 tctacatgtt tgaaaacgct tgacattgct gtagtcatgc tgttcatggt gtcttcaaaa
     2101 taccacgggc tttcgctttg agaaaccatt attccgtcgg gtttaagcgc ttttttaacg
     2161 agccggtagt aatcttcctt aaaaagtgca actccgggtc cgacaggatc gcttgaatcg
     2221 gtgatgatga ggtcaaaata gttctcatat ttgtcaagga acttaaaacc gtcttcaaag
     2281 tgcattttca ctttcgggtt ttcaagttcg catgaaaccg atggaaaata tttctgactt
     2341 aatctggtca ccctttcatc aatttcacat aatactgctt ccttgatcga ggggtgctta
     2401 agtatttctc tgatcgctcc gccgtcaccg ccgcctacaa caagaactct ttccgggttt
     2461 ttgagagtgc agagcggcat atgcgaaatc atttcgtgat atacaaattc gtctttttca
     2521 gtgagcatga caagcccgtc aagagtaagg agatttccga aaaactctgt ttcataaatt
     2581 tctattttct ggaaagggga ctgctcttcg tgaagtttcc gctttacttt cagcccaaga
     2641 cttgcttttt catcataatt ttcaacaaac cacattattt ttctcctttt aatttgctgt
     2701 cccaaagaac taccgccgca aaagctgttg caatgtttac aactttgtgc tcaatacctt
     2761 tgctttttat cgtgaaattc tttattcctc tcatttccat tgattcagcc gccatttcac
     2821 ggacttttgc ttcgacatag gaaagaggtg cgtggttatg gtgttccatg atgactccgt
     2881 ttatggatct gtcatcagga attgcgatcg caacagccgc agaaatggtt tcacccggtt
     2941 ctgaactggt tatgtcggca taggcaagag gaacaaaact tccaggagcg atgacaaatg
     3001 gctcaacttc aactgacttc ggaggaagaa tactcgacat ttttatcagg tttgtattgc
     3061 cgacacctgc ggcaagaagt gcaccgtcaa aagaagttag ttcagttctc ccttcacttg
     3121 caccggcaca gaaaaagtga atgtccggaa tgttcttcag atcgatcatg gttatttcct
     3181 ccaaaaaaaa aagatcacaa attaaagctg atgattttat atggcttgtg tgtaatgtgt
     3241 caagcatttt tacagcttgt cgcttagcac ccgttacttc ttatctttcc ctgcactttt
     3301 cacctgatgg gcaaatgcct caagccggtt gacattgtgc gttgatttca gcccttcata
     3361 aacgagtgtg cagagaggaa tgtcatccat gtcgctgcgg atgctttttg tgagtgatgc
     3421 ggtgacagtt ccgagcatgc agttgtggca catgacattt aaaattccgt ctgctccgct
     3481 ctggtggaac tcttcaattc tattaagatt taatgcaagc actttgtcaa tgtaaagatc
     3541 tgcatatctg ttcgcaactt ttttgactgc gtgaaaatcc ctttcctgcg gagttcttat
     3601 cgtttccggg aaatttctgt caacgatcca tcttgccatc tttattgcgg ggattagagc
     3661 tttcgtgaaa aagccggaga tctttttccc ttttctgtaa agttcttcat gaagctgttc
     3721 cattccgaga aatgaaacat cgatcagcga acatgccggc cagacctcaa aacccatgtc
     3781 tttaagccgg tcatagagat ggttgttgga atgctgattg atcctcgtat agacatcgcc
     3841 gacaaccccg atcttcggtt ttgttccgtc tttttctgta agatttacat tcttgaggtt
     3901 ttttacacca tttctcagag ctttgaaaaa accaccttgt atcagccctg tttcaagatc
     3961 ttcaatgatt ttccgataaa ccctgttgac ttcaccgggt gttgcttcat acggttcgat
     4021 ctctgttttc catttgaaaa aacggtctat cgtataggtt ccaagggcaa gccgcaccat
     4081 atactgagga ccaagttcct tcatgtttgt cgcatctgtt atgttcatta ctatcatttc
     4141 gccaagacca atcctgtcaa gtatcatgtg tgttgcaatg atgtactgcg gaagaaggca
     4201 tgcaccaaaa tatgaagggc cgaaatagca cgctttcgat ggatcgaatc ccggtttctg
     4261 cgcttctttt aatatgtcgc cgacaaatga aatgaaagga tgacattccc tgccgtaagc
     4321 atatttcgat ccgagattcc agctctcttc atcagtcgga ggcaggagaa ccgtcttaca
     4381 ccctgaagct ttcagtgcac cggtcattgc ataggcatgg tccatgaaag atgttatgta
     4441 gtatgtgtat tcatgcagag gcattctcgc tttgcccgga tctctgccgg ttatcacttt
     4501 tacaggttta tcattgataa tgaaatttct tgaaaatgct tcaagacgag tcacaagtcc
     4561 tgcttccgcc tgatgatcat caaattcaat gagtatgtgc ggagtatctc tgagttcata
     4621 ttcaagatac tgatcaatga acgagtcggg cccgcagccg aagtttgtga caatgacagc
     4681 tccgagattt ttgtgtcttt taagaatcct tgccgccatg ataagctttc ttccatatcc
     4741 ccaccgtact ctgtcccaga taggtggaag tttttcttcg ccaagcggaa gaaaatcgaa
     4801 aggtatcggt tcaatgttca gatctttcag ttttttcaca agattaagat tcacatgcct
     4861 gtcggtcgtg ttgtatggtc tgcctatgat tacagcacct tttctgttct cgtccgcaag
     4921 ttttctgata atttttcttc cttcttcaat acattgcaaa ccgaaattgt tctgagcagc
     4981 aaatgcggtt tttaacgctt tttcaatgac attttttctc aaaccgagct ttctggaagt
     5041 ttctgttatc cagtgtattt cctctccttc aactgggaat tcaagagtga ggatgtttgc
     5101 gttttcagtc aggttctccc tgacaatgta aggagccgcc tgagtgtagg ggcatgcata
     5161 tcgcagcgtt ttttcattcg gtgccgtaaa tagacgcaag tgaggtatga agatattttt
     5221 ataaccttta tctatcaggt tgtaaacatg accgaacagg actttcatcg gaaaacagaa
     5281 ctctgccgga acatggatga gccctttttc gacaaccgac ctttttgttt tttctgaaag
     5341 tacgggcgaa tatccgatac tagaaagaaa agtgacccag aaagggatgt gatctctgat
     5401 cgtcagagtg tccggaattg ccatttcacc aagagacggt tctgtttttg atctaattac
     5461 tgacttttcc attatttctt cacggatttt gaacatgtcg tcaaattcta catgtgttcg
     5521 cgacattttt tcaaatcttc cgcagatgct tccaaaatat gcaaaagatc cgtttgcaac
     5581 agttattttc cttatctcac agatgttttc gcataacctg cattcactgg tctcagtttc
     5641 tgcttcaact ttcaggtcat tgagcttttt caaaactttt gaaataccct gttcatattc
     5701 agtcattgcg gtcattgcgg ccccgatcgc tcctgaaatt tcaggatgag ggtggacaga
     5761 tattttctta ttcagaagtt tttcaaaaac tgctacgacg gctctatttt ttgcaactcc
     5821 accctggaaa actatatttt ctccgacagg gcttgaaccg acaacttttt caagatagtt
     5881 ttctccgatc gaataagcca gccccgcaca aagatcttca gttgaagttg ctctttgaag
     5941 atggtgaaca aggtctgaat ccatgaaaac agtgcatctg cttccaagcc gtaccggatt
     6001 tctggatttt aatgcaaggt cggcgaactc gttttttatg ctgatcttca atctgcctgc
     6061 ctgttcttca agaaatgagc cggttcctgc ggcacaggca cggttcattt tgaaagtctc
     6121 gatgttcccg ttttttatcc ttacgaactt tgaatcctgt ccgcctattt cgattattgt
     6181 gtctgaatcg gaaaagtaat gtgaacatga aaccgactga gcggatattt catccattgt
     6241 cgttccgcct gttattatcg aagcgagatg tcttccggaa ccggttgaaa cgatgtttga
     6301 tacagatatt tcattttttg accctgatat cattttctca atgaggtttt tcagtgcctc
     6361 aacaggtttc ccggcagttg gaacataacc cgaagcaaca agtttctttt ctgaatctat
     6421 aacagcccct tttgtgctga cacttcctat gtcaatcccg agaaaaaaag gttgtttcag
     6481 cggtatattg ctgatctcat tcggaatgtc gcctgaaata aaaatgtctg aaagtggttc
     6541 acggaactct tcgttttcat ggattattga tctgttctca agtttgttaa tgagttcatg
     6601 gatcgaaacc ggttcttcaa gcatgttttc tttagcgatc actgaacttc ctaaagcacc
     6661 cataacatca aaatttggag gaactatgaa ctcactctcg ttcaagctga gaatttccct
     6721 gaaagctttt accactcctg catttgcggc aactcctccc tgaaaaatga taggagctgg
     6781 gggaattttc cctttgcaca atgtcgcaag atagtttctg acaagggcaa aacagagtcc
     6841 cgcggcaatt tcatccgtag gaacagcttt ctgctgaagg tgtatcatgt ccgattttgc
     6901 aaaaacagaa catctgcctg caacacttgg aatattttga gctcttgatg caagagtact
     6961 gagttcggaa attgtgatgc cgagccgctc tgcctgctga tcaaggaaag ctcctgttcc
     7021 tgccgcacaa agttcattga aggcgtggtc tttcaaataa tgagtgccgt cattttttct
     7081 gccgatggag atgaattttg aatcctgacc gcctatttca aaaacagttt taacatcagg
     7141 atacatcgtc catgccgcag ttgcgtgggc aacgatctcg ttcactgttt ttattcccgt
     7201 gctgtttgca ataagctctt tccctgaacc ggctatcact gctccggaaa atgatatgtc
     7261 gccggattca ttcgcaatat tcttgaaaag cgacaaagtt gcatcaacag gaagccccga
     7321 agtccgcaca tatcttttga aaataatact gccggactca tcagtaataa tgcagtttaa
     7381 actgacagat ccgagatcaa gaccaagata aaaatgacca tccataaaac caccacccca
     7441 aaaaacccac attcagatat taataattga agagttgttg tcaatcttaa agagtttccc
     7501 ccgatttatt gataaaatgg atcataaacg ataagatatc tgcggtcagt tttaaaagag
     7561 gttaacatgg atgcgtttca gaaagtgctt tttgtggata cttcaaacgg tttttacaaa
     7621 ataaaacggt ataaagttgg tgactatttc ggtccggtcg atctggggat ccacatttca
     7681 gacaatcaca ggtcgctaaa tatcgggact ggactttttg ccggctcgat ctttcccggc
     7741 tcaaaccggc ttatatttac tggacattct ccatgctggg gcggtttttt catctcttcg
     7801 atgggaggat cggggcttgt ttttgataat ctcggaatta acatggtgag cattgtcgga
     7861 aaagctcaga caccttcaat tctctatctg aacagaaatc acggtgaaga gatcgaggtc
     7921 gaagttcacc ctgttgatgt cagtaaagtg tggaacagcg gcagaaaagg ggtctattca
     7981 ctttttgatc acacgcttga aaaattcggt gaccgttatg agcttgatcc gagaattctt
     8041 gcgacaggtc cttcatcagt tacaaccgat tttggaggaa tatgttcagt tccgatagtt
     8101 aaaaagaaac tcacttttgt tgacacctgg gcaggtcgcg gcgggtttgg ctcgaaaatg
     8161 tttcaggaac acggaattgt cgcaataatt tacggtggaa ccacaattga tgaggatttc
     8221 agggatagaa ctgttgccga tgagtggttt gaacagaaat ataaacagaa acttattgca
     8281 aaagattttg aaagcacgac aaaataccgt tttgatccaa agctcggaac aggcgggact
     8341 ttcggggtaa attttgcaac aattaaagac cgcatcattg ctttcaacta taaaactatc
     8401 tttgattcgg ataaatccag actggaactg acagaaaaat ttataacaga ccactatctt
     8461 ctgcagttca acgaagagac gatagaaaag aaacagcagg cgacatgcgg agaaccctgt
     8521 cctgcagtat gcaaaaagat gaacggagaa ttcaagaaag attacgaacc ttatcagaca
     8581 atggggccat tgtgcggtat ttttgatcag cgggcggctg aaaagctcaa tcatcactgt
     8641 gatgcgatgg gaatggatgc gataagcggc ggaggagtgc ttgcatggct tatggagctt
     8701 ctggataatg gaacactttc aaaagaagac ctcggtgttt caaaaatgcc gaaattcgat
     8761 cttgaaaact ttgatgttgt taaagattcg caaaacaacg cagatctcgg cattgagctt
     8821 attgacgcaa tccttaacaa atcagggata ttagatctga gtgaaggggt cagaaaatgg
     8881 gctagaaaag ttcatagatc aaccggaaaa aagataatag acagttttgt ttttacagca
     8941 aacggcagaa gagggtggat ggttcccaat cagtactgga caccgggagc gcttgccccg
     9001 atggctataa tgggcaaata ttacatgtat tacggaaatg aattccttcc gccaagaact
     9061 ctcggagttg catgtgcaga ccgcctgaaa aaagagctga tccttgacaa tgccggaata
     9121 tgcaggtttc acaggggctg ggctgaagag atgatcccgg aaatgatcga atcgatttat
     9181 gatcttaagg aaaaattcct ttcatccgtc aattttacgg cagggagaat aaattcaaga
     9241 aacgccgcag ttttccttga atcagaaagg aatatcgagt ttgtttacac atttcttaaa
     9301 agaaaacggg atgttgacgg tgaaacaaga gaagatctca actactggat agaaca
//
LOCUS       DAOOYB010000107         9302 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_8113_length_9302_cov_13.810209, whole genome shotgun sequence.
ACCESSION   DAOOYB010000107 DAOOYB010000000
VERSION     DAOOYB010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9302
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8113_length_9302_cov_13.810209"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..707
                     /locus_tag="PL056_07590"
     CDS             90..707
                     /locus_tag="PL056_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46917.1"
                     /translation="MKKFLTLFVIMALFSGFFYSCAEKQVDKRELVKELVEKGDFQQA
                     KVVLIGLRGQYPNDQTLTDLAKQVDEKTAESIYKKYWEEAEQGGSAEDWIKAMIKIKN
                     VENLNKDMVDNWIKKASEKCVDAGAKKLNDGLLLGLLDKLVQRYQVITHHDRLMYITM
                     FVKEGRFPLKEWKDTFITKYAELMDPETEEFIGWPRPEKPAEVKK"
     gene            complement(710..904)
                     /gene="thiS"
                     /locus_tag="PL056_07595"
     CDS             complement(710..904)
                     /gene="thiS"
                     /locus_tag="PL056_07595"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HPM46918.1"
                     /translation="MIKVSGREIELKSSTVSELLAQLNIDTENVAVVRNGVVIKRSVW
                     KIEPVCDGDSIEIFSPVSGG"
     gene            complement(906..3290)
                     /gene="pheT"
                     /locus_tag="PL056_07600"
     CDS             complement(906..3290)
                     /gene="pheT"
                     /locus_tag="PL056_07600"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HPM46919.1"
                     /translation="MKLSLNWLKDYIELDDISAEEISKQLTMKSAEVEAAHWTGAHFE
                     KVIIAKVENVVPHPDSDRLKLATVFDGSGRQTVVCGAPNVATGQTVAFAPLGTVLPGN
                     FEIKPAKIRGVESCGMICAEDELGLGTNHDGIMVLDGSLTAGTPVSTIFGEKDYIIEI
                     ENKTINHRPDLWGHFGIARELKAIFKRPWKKELKYTVIKSDRVEEKFEIEINTARCLH
                     YLGLKMGGLKVAPSPEWMKKRLENIGVRPINNIVDIANFVMYETGHPMHTFDRRDIAG
                     NKIVIRDAGEGEKFVTLDGLERTLKAEDAVIADTARGLAIAGVMGGLNSEVKDDTTEI
                     FIESALFHPSSVRKTSTRLDLRTDSSSRFEKALWVENCYLAMQRFVELTKELIPGAAV
                     LSDLAVADNSANYGFKGHITLTTAKIRSFLGITEEKLPDSEIVSMLSFLDFKLETKGG
                     TLVVEIPEHRRSKDVSRAEDIIEEIGRLYGYNNIEPVSPLFNMDRAPVNIDLEKGNAV
                     RNIFVNCFGASEVVNYSFSGPAELEKVPFNKEKLIETVAEKEAPFLRYSLAPAMLSNV
                     FENLKNFKDLSLFEFSRIFTADGEKKRMGFIATGEKATFDLLKSIVLSISKELRTPQL
                     RFDRIKDGFMLGDVILHPGRSAVVAFAKTELGIMGEVHPALLKKYDISVPVYYIELDR
                     EALFSLPERSVKFTPLLKFPSTGFDVTVIVPQKLEVDELFKIIKKSVDPKIFVESKLV
                     SCFAGAPIPEGFQSISFRIILNAKERTLTGDEMKNTQQKLFNDFRKAGYKISGD"
     gene            complement(3305..4321)
                     /gene="pheS"
                     /locus_tag="PL056_07605"
     CDS             complement(3305..4321)
                     /gene="pheS"
                     /locus_tag="PL056_07605"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014244500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HPM46920.1"
                     /translation="MKNQLENLRKDFLEHINSISEKEALETLRVSFLGKKGAVTSIMK
                     DLKDLSNEMKKEVGQLINQIKSDMEKTINEKHIEFEEKEIRKKIESQWSDITWPSEPV
                     QGSVHPISKVRHELEELFVSMGFEIIDGPEMETEYYNFNALNIPDDHPARDAQDTFWL
                     KNGMLLRTQTSPVQIRGMQSRKPPFKFVSPGKVYRNEAVDASHEHTFHQIEGMVVDKG
                     ISVANLIYTLKKIVSNIFGHPVEIRLRPSFFPFVEPGFELDFKCQICGGKGCSVCKQS
                     GWVEYLGCGMIHPNVLKNGGIDPEEYQGFAFGGGIERLVMMKYSIDDIRHFHGGDLRF
                     MKQF"
     gene            4476..5117
                     /locus_tag="PL056_07610"
     CDS             4476..5117
                     /locus_tag="PL056_07610"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPM46921.1"
                     /translation="MKRKIAFIGAGRDEIKFVKFIACLPEFEIALIADDRKDSAGIKY
                     AEENQIRTVTSLNEALKFHDINIFAVFSPGIDVFKFSQSVKTDQTVVDRKQFEFLYDA
                     VGSLISTRYSFVEDNFLLNTKEIKKAISDFGMITKNIDILAINASIEAARAGESGKGF
                     AVVASNIKDLVKSSREMLIHIKSILEKFTKTHQHMIDLRTALVGKESDKNESE"
     gene            complement(5123..5737)
                     /locus_tag="PL056_07615"
     CDS             complement(5123..5737)
                     /locus_tag="PL056_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46922.1"
                     /translation="MLIFFFAVTGMVLYFQQKEISLLRKKTEINAKEIEVLRIDINTK
                     KENVSHESDKQSAKNTDLFFLQRKMTEMKKEIERMKKNNDSDALNEYREKEEQTWDEH
                     TENVKKLWSGNLKQKLIEQKFNDDEIAEVLHNYSDMIDKMKAYSLSWYREEISNEELN
                     NVSVQYAKDFYESSSSLIGEQKASLVLGVVFPDPFFRKSLFDVE"
     gene            complement(5760..6611)
                     /locus_tag="PL056_07620"
     CDS             complement(5760..6611)
                     /locus_tag="PL056_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46923.1"
                     /translation="MFSVFFRGALLLPFFFLSFNSLKGDDGLSNYYALFFSDADKYDK
                     WLATDKNDRFKDIFISLYYLPDKGKRIFSNEIELRIIDSYSKNESYGYFYAEIGSQTP
                     VEFIIYGIKKGEISINGSKKGDIKIENETGYSHLKGTFEKGVYFVSVKIEKKMDGLPV
                     KVLSNKKLKLSEGRGFTKDAASRFFLTNISKKSVRNIFSKLYKGFCFPYNNTDSDSKE
                     LFFSISTRKDLTEVRSNKLIYFLSSLAGRLSVEEKLKKIGFSSIQIDWWKKSFFEREV
                     CFDERYQ"
     gene            complement(6618..>9302)
                     /locus_tag="PL056_07625"
     CDS             complement(6618..>9302)
                     /locus_tag="PL056_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46924.1"
                     /translation="ETMKELEKAAAEDAKSEETFAKKMNELSAEEQKIAASYDDKISK
                     IEEKVASIDKNYEQKMAPFNVDAIVDKMNQAIAELDNQKVEEINKHQELLDAIAQAKG
                     NAEQNYDERRAALKDNKVELSKLERDHSAAMRKMEVDEQSTQRKIQFAEASYERKIKF
                     EKSKYERDIEKNKVELKKLEKEKNLELISINKELGGLKKEKGAAIAAHQKKVAAENSS
                     HKAEIGAKAANAKKITANGLAKERQIAGSVKARKAAVEKEKVAEIAKINSEKANINNK
                     YKALADKEKGGIRAGIAEKQKRIAAIGKEIKAWEIKIKKVDPAKLKEVTDPVDKEIAN
                     LQKQKADLQTEKAAKIAALAEANKKVMADLSAKKTAAAQNTKGVDAQIKSEQAIIAKK
                     FADIRNKMKADIANLQKEKAQKIGAFKTENNATLKQKALAEGGFKKALAVELAKLSGA
                     RNKELAKLKADLAKVSKDLAVLEKSHDSFVMKEISKIDEKYEKMLTELDMKLGSENQR
                     LQNENKVFTAKKKQEKAEAEKKFKMFLADKGKFKANNDNLIKQAQAERDKKLKARKDE
                     RAKLQEKWTKEKDMRTAALDRKTAGLKSRLATNNKQIDAIAGEIDSVNNVWARKLEEM
                     KAKHHTDTKKQDAVWQKTAAAKEKWYEDTKKAINDKYDNMELKEKQDREAQKQQMMAK
                     RDKLLAEKEKRKAKRTDVLDKEKANWEKAKAKFVDQLEKLKVEIDKMNSQKSALVEKD
                     KENAKVKKDQVQKKYEETIGKINVAELKMVQQKFKKEYEEEGVREVQTTGATKKIESV
                     VAEAYAKSGLEKLEKNDIALARKDFVDALYVDRNNKTAKEGLKAITVKAQAMYWDASG
                     SKDSNKSKAVKILETLMKSLLPTDEIYLKSMLLLEELK"
BASE COUNT         2467 a         2030 c         1597 g         3208 t
ORIGIN      
        1 ggctcccttt ttttttgact ttttccaatg tttgctttac cttggttcat aagaaaggtc
       61 ggaatgagac attttttttg gaggatacga tgaaaaagtt tttgacatta tttgttatta
      121 tggccctgtt ctcaggattt ttttattcct gtgcagaaaa acaggttgac aaaagagagc
      181 tcgtaaagga gctcgttgaa aaaggggatt tccagcaggc gaaagttgtg ctgataggtc
      241 tgagaggaca atatccaaac gatcagacgc ttacagatct tgcgaaacaa gttgatgaaa
      301 agaccgctga aagtatctat aagaaatact gggaagaagc ggagcagggt ggttcagcgg
      361 aagactggat aaaagcaatg ataaagatca agaatgtaga aaacctcaac aaagacatgg
      421 ttgacaactg gataaaaaaa gcatctgaaa agtgtgttga tgcaggagcc aaaaaactta
      481 atgacgggct tcttctcggg cttcttgata aacttgttca gagatatcag gtaattactc
      541 accatgaccg gcttatgtac atcacaatgt tcgtaaaaga aggaagattc cctttgaaag
      601 agtggaaaga tacttttatc acaaaatatg cggaacttat ggatcccgaa acagaagaat
      661 tcatcggatg gccaagaccg gaaaaaccgg ctgaagtaaa aaaataattc tatccgccgc
      721 ttacaggcga aaagatctct attgaatctc cgtcacatac cggctctatt ttccagacag
      781 atctttttat gacaactccg ttcctgacta cagcgacatt ttctgtgtca atgttaagtt
      841 gtgcaaggag ttcgctgacc gttgatgact tcagctcaat ttctctgccg ctgactttta
      901 tcatttcagt caccgcttat tttataaccg gcttttctaa agtcgttgaa aagtttctgc
      961 tgagtgtttt tcatttcatc tcctgtcagg gttctttcct ttgcgttgag aattatcctg
     1021 aaagagatgc tctggaaacc ttccgggatc ggtgcaccgg caaaacagga tacaagtttt
     1081 gattcaacaa aaatctttgg atcaacagat ttcttaatta ttttaaacag ttcatcaact
     1141 tcaagtttct gcgggacaat gacagtcaca tcaaatccgg tcgacggaaa tttgagaagc
     1201 ggtgtaaatt taacacttct ttcgggaagt gagaaaagcg cttcacggtc aagctcgata
     1261 taatagacag gaacagaaat atcatatttt ttaagaagag cggggtgaac ttcacccatg
     1321 atacccagct cggtttttgc aaaagcgaca acagctgacc ttcccggatg aaggatcaca
     1381 tcaccgagca taaaaccgtc ttttatcctg tcaaaacgaa gctgaggagt cctcagttcc
     1441 tttgatattg aaagaacaat gcttttgaga agatcaaaag ttgccttttc acctgttgct
     1501 ataaaaccca tcctcttctt ttcaccatca gcagtaaata tcctgctgaa ctcaaacaga
     1561 cttaagtctt taaaattctt aagattttca aacacattgc tcaacattgc cggagcaagt
     1621 gaatatctta gaaatggagc ttccttttct gcaacagttt caataagctt ttctttattg
     1681 aagggcactt tttcaagttc ggcaggtccg ctgaaagaat aatttaccac ttcgcttgcg
     1741 ccgaaacagt tgacaaaaat gttcctgacc gcattccctt tttcaagatc aatgttaacc
     1801 ggagctctat ccatattgaa gagcggactg accggctcaa tgttgttata accgtaaagc
     1861 cgtcctatct cctcaattat atcttcagct cttgaaacat ccttgctcct tctgtgttcg
     1921 ggaatttcaa caacgagtgt tcctcctttc gtttcaagtt tgaaatcaag aaaactcagc
     1981 atcgaaacta tttcagaatc aggaagtttt tcctctgtta taccaaggaa agagcggatc
     2041 tttgcggttg taagcgtgat atgtcccttg aaaccgtaat ttgcgctgtt atctgcaaca
     2101 gcaagatcag aaagcactgc cgcacccgga ataagttctt ttgtgagttc aacaaatctc
     2161 tgcatcgcaa gatagcagtt ctccacccag agcgcttttt cgaatctgct tgaagaatct
     2221 gtcctgagat cgagccgggt cgaagttttt cttacacttg aaggatggaa aagggcggat
     2281 tcaatgaaaa tttcagttgt atcatccttt acttcgctgt taagaccacc cattacacca
     2341 gctatcgcaa gtcctctggc ggtatcggca ataactgcat cttccgcttt aagcgttctt
     2401 tccaaaccat caagagtaac aaatttttca ccttcacccg catccctgat gactatttta
     2461 ttccctgcaa tgtcgcgtct gtcaaaagta tgcatcggat gacctgtctc gtacatgacg
     2521 aaatttgcaa tgtcaacaat attgtttatc ggtctgacac caatgttttc aagccgtttc
     2581 ttcatccatt caggagatgg agcaaccttg agtccgccca ttttcaatcc gagataatga
     2641 agacatcttg ctgtatttat ttcaatttca aacttttctt caacacggtc gctcttgata
     2701 accgtatatt taagttcttt tttccagggc cttttgaaaa tagctttcag ttctcttgcg
     2761 ataccgaaat gaccccagag gtccggtctg tgatttatcg tcttgttctc tatttcaatt
     2821 atgtaatcct tttcaccgaa aattgtcgaa acaggagttc ccgccgtaag cgaaccgtca
     2881 agcaccatta tgccgtcgtg attcgttccg agtccaagct catcctctgc acagatcata
     2941 ccgcagcttt caactccccg tatctttgca ggttttattt cgaaatttcc gggaagaacc
     3001 gttcccagag gcgcaaaagc aacagtctgt ccggttgcca cattcggagc tccgcataca
     3061 accgtctgcc ttcctgatcc atcaaaaacc gtagccagct tcagtctgtc ggaatcagga
     3121 tgcggcacta cattctccac tttggcgatt attacctttt caaagtgggc accggtccag
     3181 tgagccgcct caacctcagc agacttcatt gtaagctgtt ttgatatctc ttccgccgag
     3241 atgtcatcaa gttcaatata atctttaagc cagttaagag ataatttcat tttcagtctc
     3301 caagctaaaa ctgtttcata aaacggagat caccgccgtg gaaatgacgg atgtcatcaa
     3361 tactgtactt catcataaca aggcgctcta ttccaccgcc aaacgcaaag ccctgatatt
     3421 cttcaggatc tattccgccg tttttcagaa cattgggatg tatcattccg catccaagat
     3481 attcaaccca gccggactgc ttgcagacgc tgcacccttt tcctccacat atctgacatt
     3541 tgaaatcaag ttcgaatccc ggctcaacga acggaaagaa agagggtcga agtcttattt
     3601 caacagggtg tccgaaaata ttagaaacta ttttctttaa tgtatatatg aggttcgcaa
     3661 cagaaatacc tttgtccact accattcctt cgatctgatg aaaagtgtgt tcgtggcttg
     3721 catcaactgc ttcgtttcta tagacttttc cgggagatac aaacttaaaa gggggctttc
     3781 tggactgcat tcctcgtatc tgtacaggtg aagtctgagt tctaagaagc ataccgttct
     3841 tcaaccagaa agtgtcttga gcatctcttg cagggtgatc atccggaatg ttcagagcat
     3901 tgaaattata gtattctgtt tccatctcag gcccatcaat tatctcaaat cccattgaaa
     3961 caaaaagctc ttcaagttca tgcctcacct tggagatcgg gtgaacggaa ccttgaacag
     4021 gttcactggg ccatgttata tcgctccact ggctctcaat ttttttgcga atctcttttt
     4081 cttcaaattc gatatgtttt tcatttatgg tcttttccat atctgatttt atctggttta
     4141 ttaactgtcc gacttccttt ttcatttcat ttgaaagatc cttgagatct ttcattatcg
     4201 aagtgaccgc acccttctta ccgagaaaag agactctcaa tgtttccaag gcctcttttt
     4261 cactaattga attgatgtgc tctaaaaagt ccttccttaa attttccagt tgatttttca
     4321 tgaaccgacc tctgaatctg aatctgttaa ttcaatacga cagcaacaat atgaaaatac
     4381 taataaactt ggttttttca agaaaaaaga actcttaaag tttgaactat cctgaaaata
     4441 tgctaatatc agtcaactta tttgtggagt tcaaaatgaa aagaaagatt gctttcatag
     4501 gtgccggcag agatgaaatt aaatttgtaa agttcattgc atgccttcct gaatttgaaa
     4561 ttgctctaat tgctgatgac cgcaaggatt ccgcaggcat caaatatgct gaagaaaatc
     4621 agataagaac ggtaacttcg ctcaatgaag cactcaagtt ccacgacatc aacatatttg
     4681 ccgtcttttc ccccggcatc gatgttttca aattttcaca gtctgtgaaa accgatcaga
     4741 ccgttgttga caggaaacag tttgaattcc tgtatgacgc tgtaggatca cttatttcta
     4801 caagatattc gtttgtggaa gacaatttcc ttctcaacac caaagaaata aaaaaagcta
     4861 tatctgattt cggtatgata acaaaaaaca tcgacatcct tgcgataaac gcttccattg
     4921 aagctgcaag ggctggtgaa tcaggcaaag ggtttgctgt tgttgcatca aacataaagg
     4981 atctcgttaa aagcagcaga gaaatgctga tccacataaa atccatcctt gaaaaattca
     5041 caaaaaccca tcagcatatg atagacctca gaactgctct cgtgggcaaa gaatcagata
     5101 aaaacgaatc agaatgatat tgtcactcaa catcaaaaag actttttcta aaaaatggat
     5161 cgggaaaaac gaccccgagc accaatgaag ctttctgctc tccgatcaaa gacgatgaag
     5221 attcgtaaaa atctttagca tattgaacag aaacattgtt cagttcctca ttagagatct
     5281 cttccctgta ccagcttaaa gaatatgctt tcattttatc tatcatgtca ctgtaattgt
     5341 gcagaacttc cgcaatttca tcatcgttga atttctgctc gatcagtttt tgcttcaaat
     5401 ttccgctcca tagtttcttt acattttcag tatgctcatc ccatgtctgt tcctcttttt
     5461 ccctatattc atttagagca tctgaatcat tattcttttt cattctttca atttcttttt
     5521 tcatctctgt catttttctt tgaagaaaaa aaagatctgt attttttgcg gactgtttat
     5581 cgctttcatg agatacattt tctttttttg tatttatatc aattcttaga acttctatct
     5641 cttttgcatt aatttctgtt ttttttctaa gcagagatat ctctttctgc tgaaaataca
     5701 gaaccattcc tgtaacagca aaaaagaaga ttaacactgc aatctgccaa actgattttt
     5761 tactggtacc tttcatcaaa acacacctct ctttcaaaga aacttttttt ccaccaatcg
     5821 atctgaattg atgaaaaccc gatctttttc aacttttcct caactgataa ccttccggca
     5881 agagaagaaa gaaaataaat aagtttattt gatctgacct ctgtcagatc ttttctcgtt
     5941 gatattgaaa agaacagctc ttttgaatcg ctatccgtat tattatatgg aaaacaaaac
     6001 cctttataaa gttttgaaaa aatattgcga actgactttt tgctgatatt tgttaaaaaa
     6061 aatctgcttg cagcatcttt tgtaaaacct cttccttctg aaagtttcag ctttttattt
     6121 gaaagaactt ttaccggaag cccatccatc tttttttcta tttttacaga aacgaaataa
     6181 actccttttt caaatgtgcc ttttaaatgt gaatagcccg tctcattctc aatttttata
     6241 tctcctttct ttgaaccgtt aatggagatc tctccttttt taattccata tattataaac
     6301 tcgacaggtg tctgagagcc gatctcagca taaaaatatc cgtatgattc gttttttgaa
     6361 taactgtcaa tgatcctgag ttctatttcg ttggaaaaaa ttcttttacc tttatcggga
     6421 agataataca gggaaatgaa tatgtcttta aaacggtcat tcttatcagt tgctaaccat
     6481 ttgtcatact tgtccgcatc agaaaaaaaa agagcataat aatttgacag accgtcgtca
     6541 ccttttaatg aattgaaact taaaaaaaag aaggggagca gtaacgctcc cctgaaaaat
     6601 actgaaaaca tttgatttta ttttagttct tcaagtaaca acattgattt caaataaatt
     6661 tcatctgtcg gaagaagtga tttcataagt gtctcaagta tcttgacagc tttggatttg
     6721 tttgaatctt ttgacccgga ggcatcccag tacattgcct gtgctttgac agttatcgct
     6781 ttaagtcctt cttttgcagt cttattgttt ctgtcaacat aaagagcatc aacgaaatcc
     6841 tttcttgcaa gggcaatatc attcttttca agcttttcaa gtcctgattt agcataagct
     6901 tcggcaacaa cagattcaat ctttttggta gctccggtag tctgaacttc tctaacacct
     6961 tcttcctcgt attccttctt gaatttctgt tgaaccatct tcagttcagc gacatttatt
     7021 ttgccgatag tctcttcata ctttttctga acctgatctt tcttgacttt tgcattttcc
     7081 ttgtcttttt caacaagagc gctcttctgg gaattcatct tgtcaatttc gactttgagt
     7141 ttttcaagct gatcaacaaa ttttgctttt gctttttccc agttcgcctt ctctttatca
     7201 aggacatcgg ttctttttgc ctttctcttc tccttttctg caagaagctt gtctcttttt
     7261 gccatcatct gctgtttctg ggcttctcta tcctgttttt ccttaagctc catgttgtca
     7321 tatttgtcat taatggcctt tttggtgtct tcataccact tttcctttgc agcggcagtc
     7381 ttctgccata cagcatcctg cttttttgta tcggtatgat gtttcgcctt catttcctca
     7441 agcttccttg cccatacatt attaacgctg tcaatttcac cagcaatagc atcaatctgc
     7501 ttgttgttgg tggcaagacg ggatttgagc ccggccgttt ttctgtcaag agcggcagtt
     7561 ctcatgtctt tttcctttgt ccacttctcc tgcagtttag ccctttcgtc ttttcttgcc
     7621 ttaagctttt tatctctttc ggcttgtgcc tgttttatga ggttatcatt gttggcctta
     7681 aatttcccct tgtctgcaag gaacatcttg aatttctttt cagcttccgc cttttcctgt
     7741 tttttcttgg ctgtaaaaac cttgttctca ttctgcagtc tctggttttc agatccgagc
     7801 ttcatatcaa gttctgtcaa catcttttcg tatttttcat ctatctttga aatttccttc
     7861 ataacaaaac tgtcatgcga tttttcaaga acagcaaggt cttttgaaac ttttgccaga
     7921 tctgccttca gttttgcaag ttccttgttc cttgcacctg aaagttttgc aagttcgaca
     7981 gcaagagctt ttttgaatcc cccttcagcc agagctttct gtttaagggt tgcattattc
     8041 tcagttttaa aagcaccgat tttctgtgct ttctcttttt gaaggtttgc aatgtcagct
     8101 ttcattttgt ttctgatatc tgcaaatttc tttgcgataa tcgcctgttc actcttaatc
     8161 tgagcgtcaa caccttttgt gttctgggct gctgctgttt tctttgcaga cagatccgcc
     8221 attacttttt tgtttgcttc cgcaagtgca gcgatctttg ccgctttttc tgtctgaaga
     8281 tctgctttct gcttctgaag atttgcgatt tctttatcaa ccggatctgt tacttcttta
     8341 agttttgcag gatcgacttt ctttattttg atctcccatg cttttatctc ttttccaatg
     8401 gctgcaattc ttttctgttt ttcggcaata cctgctctta ttccaccctt ttccttgtcg
     8461 gcaagggcct tatatttgtt gttgatattc gccttttcag aattgatctt tgcaatttca
     8521 gccactttct ctttttcaac agcggctttt cttgctttaa cgcttccggc gatctgcctt
     8581 tcttttgcca atccgttagc tgttattttc tttgcattag cggcttttgc accgatctca
     8641 gctttatgag atgaattttc tgcagcaact ttcttctggt gagctgcaat tgcggcacct
     8701 ttttcttttt tcaatccgcc aagttctttg ttaatagaaa tcagttcaag gtttttttct
     8761 ttctcaagtt ttttaagttc cactttgttc ttttcaatat cacgctcata ttttgatttt
     8821 tcaaacttaa tctttctttc ataacttgct tcagcaaact gaatttttct ctgagttgac
     8881 tgttcgtcaa cttccatttt tctcattgcg gcagagtgat ctctttcaag ttttgaaagt
     8941 tcaactttgt tatctttaag agctgctctt ctttcatcat aattctgctc agcgtttcct
     9001 ttcgcctgag ctattgcatc aagaagctcc tgatgcttgt tgatctcttc gactttctga
     9061 ttatcaagct ccgcaatggc ctgattcatt ttatcaacta tggcatcaac attgaaagga
     9121 gccattttct gctcataatt tttatcaatt gaggcgacct tttcttcaat ctttgatatt
     9181 ttgtcatcat aagaagccgc tattttctgt tcttctgcag aaagttcatt cattttcttt
     9241 gcaaaagttt cttctgactt tgcatcttcg gcagcggctt tttcaagctc tttcattgtt
     9301 tc
//
LOCUS       DAOOYB010000108         9146 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_8312_length_9146_cov_12.229898, whole genome shotgun sequence.
ACCESSION   DAOOYB010000108 DAOOYB010000000
VERSION     DAOOYB010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9146
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8312_length_9146_cov_12.229898"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..1749
                     /locus_tag="PL056_07630"
     CDS             76..1749
                     /locus_tag="PL056_07630"
                     /inference="COORDINATES: protein motif:HMM:NF023048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HPM46925.1"
                     /translation="MKKLLILLAVSVSFVLFSSCTVEDDEGTDNENVVGDENNSKDDS
                     NTKNDSDTVDNKDDSATESDNEKTDEGGMYIECTPGETRECYEGPSGSKGVGACKAGL
                     STCVVDGTDWSECVGQVTPGPEICGDGIDQDCDGSDMTAETAIDIDGDGYTYCDGDCC
                     ETSWDCRGEPDKINPGSYEFDDNMIDDNCNGEVDEKTICDEGLALAVNDYPGNAEKLA
                     KGMGVCTGLISAELSLAGDPVPELIADEQCGGNDGTSYTKANRESLTMPYYDNKYKTF
                     AVEPVFGTAMTPLEGSALSILSTADWDQPTQDAACATLDAGDMKTSSKIPEDWINMMP
                     GCEIPKAPSCGGQPPQSGLTNQCQGKSIPSVQDPIMLTLKLKVPSNARAFEFNLFFMS
                     IEYPTTVCSSENYNDFFIALIDSTYNSKNPDAEFQNPYDKNLAKDELGNPVGVDLAPA
                     GLFKACHPSCGGALSNTNPYGFCEGDQILAGTGFEAKTVMGMCSGSGGTGWLKTTGNV
                     EPNEEITLRLALWEQGSVAYGPDHSWDSTVVLDGFKWLPLPGKPGTQEK"
     gene            complement(1809..3980)
                     /locus_tag="PL056_07635"
     CDS             complement(1809..3980)
                     /locus_tag="PL056_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46926.1"
                     /translation="MRTVQVFTSIFLFIVFFIWSFGGSYGALINFYFPYLPIFFFIVL
                     VPFSIPGFSEKFFKILSKLEKHSFVIFPAFIFIFTQYVWHDVFSGIPHVQDEINFKYL
                     ATAILDGSLTSPLHPHYEFFRFLYIIPSESGTYSIYDIGYPIVLAPFLYFKVPFLLNP
                     LLSSFSIYFSGRIAEELYDRHTASLSMMLASFSLFLSSMGGTWMSHSLCAFLTLGAVL
                     FAIKSLKKDFVRNTVISALLISALMFSRPQNALFVLFSIVLFYAFFAGGRNFIKSMLI
                     SGSIIFLFFLLFNYTHYIFSGAVLTGKHSVYWNISEPVDDCMRLGLGNGCRHATLIDL
                     PAEGLTFTHAVYTTYLRLNQLVYGMFFHPLMFIFIVILFLFRTDSDKFRKDMFILSCF
                     LIIFFTYFFFYFDGNVYGPRYYYEAAFFLMILASRGIMLFFNKFNIKSKNPLLRPVNL
                     FSAFFVAGIIFQYFAAAPAFFKPHRLAFWGADPLLYKALEEKNIKKGVIFINPHSFYS
                     SGTATMNLHKIDKNDLIFARDLGPFQNRRLMNHYPEKSAYIVNFNKEWFEERLPDIIE
                     LKKEELPDIMSVELEDKEWPLSGSPDYCNTYPAWKYIDQFAGFELEPELTAGRKYFFC
                     RFLDNSDHYSFGQYFEKEGRYAVKIHHVKTPVGAKFRISSGKSRNDVNFSDVENTLSE
                     TEFEMDFEKGMNIIKIQPRNIKEKKSGYFIIDRIEFQMTSD"
     gene            complement(3977..6385)
                     /locus_tag="PL056_07640"
     CDS             complement(3977..6385)
                     /locus_tag="PL056_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46927.1"
                     /translation="MKNAIYSAVFLLSLTGLALENALTRIFSITMWYHYAFMAISVAM
                     LGMSTGAVKVYVSEFGKLSREKIEDLICIYSRMFALFTIISLFALLSIPFIPRPTGVG
                     MFTTWFIYAIAAVPFYFAGVAVALIITTKYIENVNRLYAFDLAGAAVGSVLFFIMLSF
                     TDAVTFIIFLASSGFLASFFISRKKIDVVLFLAVIVFSLINHSEKIFRIEWVKVEKGV
                     LTATVENNVEWERWTPFSRITVSPMLESFGFGWGISGKMMSHYPDYRIEQKALTIDSA
                     AATVITKNQMPVEGLIHLKYDITNIVHPLFPNSRVGVIGVGGGRDILSSLHFNQKEVW
                     GIEINGRILEAVTEHYSDYTYDIKKLPNVHLVEDEARNFFEKNDIKFDIIQASLIDSW
                     AATASGAFVLTENSLYTVEAWVAFLSKLSDNGAITMSRWYYPKRPGELLRLVNLAYEA
                     LLKSGVKEPRKHIVLAGLNFFDPASPIDEQYGSGTIVVSKKPFKENIVDRFITICDTY
                     GFEKLIVAGREDDSVFKELVTPGKRDSFVAGYSLDITPPTDDNPFFFNMLRPLSVLKG
                     DNIESEGPLSTNLMAVKNLLSLVAIVVLLSIFLIIVPLIAKMKLKTKEALFNRHSAYF
                     VSIGTGFMLIEIAFIQKFAIFLGHPTYSILVVLFTVLLFSGTGSFFSGAIYKKTGMKG
                     LFTIIVLTIAFTGSINMFILPYLSSWTILPRIIYSFSVMAVTGFVLGMPFPTGMSLLN
                     EKMKERAPWLWGVNGAASVVSSVAAVSISLFYGISATFFTGLFFYFTALISGILIEKD
                     FIKK"
     gene            complement(6466..7650)
                     /locus_tag="PL056_07645"
     CDS             complement(6466..7650)
                     /locus_tag="PL056_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46928.1"
                     /translation="MKKFLILLFISLFLLSCGDKDQKSQDSENNDESTDEAVDVDESV
                     SDKINELSDVDEIENNGDTGNTGNTGNTGDSGDSGNTGNTGDTAGDEDEETPDEDEEL
                     EIPAGTIGFETGMPERGIYSKGFTYSVDVMGDLVDLYVPLSNTDKMSTAIYMQGAKVD
                     KGFYSIFAKALSAYGFVVAVPNHKSFTGDNMTENKVFTQVWNYIKQMSGESGSHLFNR
                     VDVAKVTVLGHSFGGVASLGILQNKCEIPTCTGTYNHPEELSAAVLYGTNTKNPYLGG
                     VAEVNTGNIPTVFVQGLLDGKAKYEDMTATFEKTTGSPVSAVKVTGANHYGICDINNP
                     AGAEEDSNIPALDQLTGINIIAKWSGIFMRAHIFGDSDDHDFFYTGTGDTADSVVEII
                     SK"
     gene            complement(7689..>9146)
                     /locus_tag="PL056_07650"
     CDS             complement(7689..>9146)
                     /locus_tag="PL056_07650"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HPM46929.1"
                     /translation="DSGDSGNTGNTGDTGDTGNTGNTGNTGDTGNTGNTGDTGNTGNT
                     GDTGDTGNTGNTGNSGDTAVNITLVENVIDDNPYNPAFVTVADLDVDGNLDIISCEYG
                     PSGFSNTSMEGKIQIYYGTGNIATWNKQQLDTGTAVKFPHPPVVADVDGDTDLDLIIP
                     GGFLACEANPLGGPCGTIFWMENKGGTWERHDIVPMGDPSFYFGIEYVDFDGDGIKDI
                     VTTAEKFTKNMMGSITAREEKLLWFKGTDSGIRFDTTKKFISESHGGPFSRVIDIDED
                     GDLDIVAAQYFIDPEAQPSFVWLERVAIDNWTPHAINNDSGEGFQINIVDDLYGDGVR
                     RAVGTNHTNTAANSSSAMEGLFVFDIPEDPTQPWTKKLISDGIKSRKGSSMAPMAAPG
                     VFGIGDLTGNGLMDIALSGDGDARIFWYQQTSAGEFTQHVIGGEPVGSQPEAPNSQFG
                     QAGGMKIVDLDDDGQSELIVTLYESNKLIIYTVQK"
BASE COUNT         2810 a         1966 c         1827 g         2543 t
ORIGIN      
        1 cttgatattt catcaactct ttatataatt ccattcagca gtgtgtgttt ttacatattt
       61 tttccggagg acataatgaa aaagctttta attcttcttg ctgtatcggt ttcatttgtt
      121 ttattttctt cctgtactgt tgaagatgac gaaggaacag ataacgaaaa tgttgtaggt
      181 gatgaaaaca actcaaaaga tgacagcaat acaaaaaacg attcagacac agttgataac
      241 aaagatgatt ccgcgactga atccgacaat gaaaaaaccg atgaaggggg aatgtatatt
      301 gaatgcactc ccggagaaac aagagagtgt tacgaaggac caagcggctc aaaaggggtc
      361 ggagcatgca aggcagggct gtcaacctgt gtcgttgacg gaactgactg gagcgaatgt
      421 gtcggtcagg taactccggg accagaaata tgcggagacg gaatagatca ggactgcgac
      481 ggaagtgaca tgacagcgga aaccgccatt gatattgacg gtgacggcta cacatattgt
      541 gatggcgact gctgtgaaac ttcatgggac tgcagaggtg aaccggacaa gataaacccg
      601 ggatcttatg aatttgatga caatatgatc gatgacaact gcaacggcga agttgacgag
      661 aaaacaatat gtgatgaagg acttgcgctc gcagttaatg attacccagg aaacgccgaa
      721 aagcttgcaa aaggaatggg ggtatgtaca gggcttattt ctgctgaact ttcgcttgca
      781 ggagatcctg ttccggaact gatagcagat gagcagtgcg gaggcaacga cggaacaagc
      841 tatacaaaag caaatcggga aagtctcaca atgccttatt atgacaacaa atataagaca
      901 ttcgcagttg agccggtatt cggaacagct atgacaccgc ttgaaggatc agcactttca
      961 atcctttcaa ctgctgactg ggatcagccg acgcaggatg cggcatgtgc aacccttgat
     1021 gccggtgaca tgaaaacttc aagtaaaata cctgaagact ggataaatat gatgccggga
     1081 tgcgaaattc ccaaagcacc ttcatgcgga ggacagccgc ctcagagtgg actcaccaat
     1141 cagtgtcagg ggaaatcgat tccttctgtc caggatccga taatgctgac acttaaactg
     1201 aaagtgcctt caaatgcaag agcttttgaa ttcaaccttt tctttatgtc gatcgaatat
     1261 ccgacaacgg tatgcagctc ggaaaattac aatgatttct ttatcgccct tatagattca
     1321 acatataatt caaaaaaccc ggatgctgag tttcaaaacc cttatgacaa aaaccttgca
     1381 aaggatgaac tcggaaatcc tgtcggagtt gaccttgcgc ctgccggtct tttcaaggcc
     1441 tgtcacccaa gttgcggcgg agcactttca aacacgaacc cctacggatt ctgtgaaggt
     1501 gaccagatac ttgcaggaac cggttttgaa gcaaaaaccg ttatgggaat gtgtagtgga
     1561 agcggtggaa ccggctggct caaaacaacc ggaaatgttg aaccgaatga agaaataact
     1621 ctccgccttg cactctggga acagggaagt gtcgcatacg ggccggatca ctcttgggat
     1681 tcaacagttg ttcttgacgg attcaaatgg cttccattac ccggcaaacc gggaactcag
     1741 gaaaagtaat tcgtgaacta gtcgtgaact gtgagtcgtg aacagatgac gggtgaaaag
     1801 agaaccgatc aatctgaagt catttgaaat tctattctgt caataataaa ataacctgac
     1861 tttttctctt taatgttccg gggctgaatt tttattatgt tcatcccctt ttcaaaatcc
     1921 atttcaaatt ctgtttctga taatgtattc tcaacatctg aaaaattaac atcatttcgg
     1981 gatttaccgc ttgagatcct gaattttgct ccgaccggag ttttgacatg gtgtatttta
     2041 acagcgtatc tcccctcttt ttcaaaatac tgtccgaatg aataatggtc agaattatca
     2101 aggaatcgac agaaaaaata ttttcttcct gcagttagtt ccggctcaag ttcaaatcct
     2161 gcaaactgat caatgtactt ccacgccgga tatgtgttgc agtaatcagg acttccgctt
     2221 aacggccact ccttgtcctc aagttcgaca ctcattatgt ccggtaactc ctcttttttc
     2281 agctcaatga tgtcgggaag cctttcttca aaccactctt tgttgaaatt gacaatatat
     2341 gcgctctttt caggatagtg gttcataagg cggcgattct gaaacggccc cagatcacgg
     2401 gcaaagatca gatcgttttt atcaatttta tgaaggttca tcgttgctgt acctgaactg
     2461 taaaaactgt gcggatttat gaaaatgacc ccttttttta tatttttctc ttccaatgct
     2521 ttataaagaa gagggtcagc tccccagaaa gcaagacggt gcggcttaaa aaatgcagga
     2581 gcggccgcaa aatactgaaa aattattcct gcaacaaaaa acgctgaaaa aagattgacc
     2641 ggtcttaaaa gcggattttt gctctttatg ttgaatttat taaagaaaag cattattcct
     2701 cttgatgcaa ggatcatgag aaaaaacgcc gcctcgtaat aatatctcgg cccatataca
     2761 tttccgtcaa aatagaaaaa gaaatatgtg aaaaagatta tgagaaaaca cgaaaggatg
     2821 aacatatctt ttctgaactt atcagaatct gttctgaaaa gaaaaagaat gacgatgaaa
     2881 atgaacatca gcggatggaa gaacattccg tagacaagct gattaagccg cagatatgtc
     2941 gtatagaccg catgggtgaa tgtgagtcct tctgccggca gatcgatcag tgtggcatgt
     3001 cggcatccat tgccaagtcc aagccgcata caatcatcaa ccggttccga aatgttccag
     3061 taaactgagt gtttaccggt aagaactgct cctgaaaaga tgtagtgcgt gtagttaaaa
     3121 agaaggaaaa aaagaaaaat tatagatccc gaaatcagca tacttttaat aaaatttctg
     3181 ccaccggcaa aaaacgcata gaaaagaacg atcgagaaca acacaaaaag agcattctgc
     3241 ggtcttgaga acatcagtgc cgatattaaa agcgcagaaa tcacagtgtt ccttacaaaa
     3301 tcttttttta aactctttat tgcaaagaga acagccccga gtgtcagaaa agcacagagc
     3361 gaatggctca tccatgttcc gcccatcgac gaaagaaaca gagaaaaaga tgcaagcatc
     3421 attgatagag aggccgtgtg tctgtcataa agctcttcag ctatcctacc tgagaaatat
     3481 attgaaaatg atgaaagaag cggatttaac agaaacggaa ctttgaaata aaggaatggt
     3541 gcaagaacta taggataacc gatatcataa attgaataag ttcctgattc tgatggaatt
     3601 atatagagga acctgaaaaa ctcgtagtga ggatgaagag gcgaagtgag agaaccgtca
     3661 agtattgctg ttgcaaggta tttgaaattt atttcatcct ggacatgcgg aatccctgaa
     3721 aacacatcgt gccacacata ctgggtgaaa atgaaaatga atgctggaaa aataacaaat
     3781 gaatgttttt ccaactttga caggatctta aaaaactttt cagaaaaccc gggaatggaa
     3841 aaagggacaa gcacaatgaa aaaaaatatc ggaagataag gaaaataaaa attgatcaga
     3901 gcaccgtaac ttccgccaaa actccagata aaaaagacga tgaaaaggaa tatggatgta
     3961 aaaacctgaa ccgttctcat ttttttataa aatctttttc aatcagaatc ccgctaatta
     4021 acgccgtaaa gtagaaaaaa agtcctgtga agaaagttgc tgaaattccg taaaacaatg
     4081 aaattgaaac tgccgcgact gaactgacaa cgcttgccgc accgttcaca ccccagagcc
     4141 atggagcccg ttctttcatt ttttcattca gtagtgacat acccgtcggg aaaggcattc
     4201 cgagaacaaa tcctgtaacg gccataacag aaaagctgta aataattctt ggaagtattg
     4261 tccacgaaga gagatacggc agaatgaaca tatttattga accggtgaat gcaatggtca
     4321 gaactattat tgtaaaaagt cctttcattc ccgttttttt atatattgca ccgctgaaaa
     4381 aactccctgt cccgctgaaa aggagcactg tgaaaaggac aacaagtatg ctgtatgtag
     4441 gatgaccgag aaatattgca aacttctgga taaatgcgat ctcaataagc atgaaccccg
     4501 ttccgatcga aacgaaatat gcggaatgtc tgttgaaaag agcttctttt gttttaagtt
     4561 tcatttttgc tataagagga acaattatga gaaaaattga gagaagaaca acaattgcaa
     4621 caagtgaaag gagatttttc actgccatca gattggtcga aagaggacct tcggattcga
     4681 tgttgtcacc ctttaaaact gaaaggggcc tgagcatgtt gaaaaagaac gggttgtcat
     4741 ctgtcggagg agtaatgtca agggaatagc cggcaacaaa actgtccctt ttcccgggtg
     4801 tcacaagttc tttaaaaaca gaatcgtcct cccttccggc cactataagt ttttcaaaac
     4861 cgtatgtgtc acagatcgtt atgaaccggt ctacaatgtt ttctttaaac ggtttttttg
     4921 aaacaacaat ggttcctgat ccgtattgtt catcaattgg agatgccgga tcaaaaaaat
     4981 tcagaccggc aagaacaatg tgtttacggg gctctttcac gcctgattta agaagtgctt
     5041 catatgcaag attaacgagc cggagaagtt ctccaggtct tttcggataa taccagcggc
     5101 tcatggttat tgcaccgtta tctgacagtt ttgacaggaa tgcaacccat gcctcgaccg
     5161 tatagagtga attctctgtc agaacgaaag cacctgatgc cgttgccgcc catgaatcga
     5221 tcaagcttgc ctgaattatg tcgaatttta tatcgttctt ttcaaaaaaa tttcttgcct
     5281 catcctcaac aagatggaca ttcggaagtt tttttatgtc gtaagtgtaa tctgaataat
     5341 gttccgtcac tgcctcaagt atcctgccat ttatctctat gccccacact tctttctgat
     5401 tgaaatgaag tgacgaaaga atatccctgc cgcctccgac tcctatcacg ccaaccctgg
     5461 aattcgggaa aagaggatgg acaatgttag ttatgtcata tttaagatga atcagccctt
     5521 cgacaggcat ctggtttttt gttattactg ttgccgctgc tgaatcaatg gtcagcgcct
     5581 tctgctcaat cctgtaatca ggataatgac tcatcatttt tccagagata ccccatccga
     5641 aaccgaaact ctccaacatg ggtgaaacag taattcttga aaaaggggtc catcgttccc
     5701 attccacatt gttttcaact gttgccgtaa gaactccctt ttcaactttt acccattcaa
     5761 tacgaaatat cttttctgaa tggtttatta agctgaaaac tattactgca agaaacagaa
     5821 caacatcgat cttttttctt gaaataaaaa aagatgccag aaatccggaa cttgctaaga
     5881 aaattatgaa tgttacagca tcggtaaatg aaagcattat gaaaaaaagg actgacccga
     5941 ccgctgcacc cgcaagatcg aaagcataga gccggttcac attttcaata tattttgtcg
     6001 ttattatcag agcaacggca actcctgcaa aataaaaagg gactgcggct atcgcataaa
     6061 tgaaccatgt tgtgaacatt cctactccgg tcggtcttgg aatgaacgga attgaaagaa
     6121 gtgcaaaaag cgaaatgatc gtgaaaaggg caaacattct gctgtaaatg catatcagat
     6181 cctcgatctt ttccctgctt agttttccga attcggaaac ataaaccttc acagcacccg
     6241 tactcattcc aagcatcgca acagatatcg ccataaaagc ataatgatac cacattgtta
     6301 tgctgaaaat cctcgtcagg gcgttttcaa gcgcaagacc ggtgagagac aaaagaaaga
     6361 ccgcagaata aatagcattt ttcataaaac aacctcaata aacttaaaat cgggtgatga
     6421 tacctgaaac agatatatga gtcaataagt tgtctgaact tgcagttatt ttgaaatgat
     6481 ctcaaccaca ctgtctgctg tatcaccggt tccggtgtaa aagaaatcgt ggtcatcact
     6541 gtctccgaaa atgtgggcac gcataaaaat cccgctccat tttgcgatga tattaatgcc
     6601 cgtcaattga tcaagagcag gaatatttga gtcttcttca gcacctgcag gattgtttat
     6661 atcacagatc ccgtaatggt tcgctccagt tactttaact gctgaaaccg gagaaccggt
     6721 cgttttttca aatgttgctg tcatatcttc atattttgct tttccgtcaa gaagaccctg
     6781 aacaaaaaca gttggaatgt ttcctgtatt tacttctgca actcccccta aataagggtt
     6841 ttttgtgttt gtcccataca gaacagcggc agacagctct tcagggtgat tgtatgttcc
     6901 tgtacaagtc ggaatctcac atttattctg aagtattccc aaagatgcaa ctcctccgaa
     6961 cgaatgacct aaaactgtaa cttttgcgac atctaccctg ttgaatagat gcgatccgga
     7021 ttctccgctc atttgcttaa tataattcca tacttgagta aacactttgt tttcagtcat
     7081 attgtcaccg gtaaaacttt tatgattagg aaccgcaaca acaaaaccat atgcagaaag
     7141 ggcttttgca aaaattgaat aaaacccttt gtctactttt gcaccctgca tatagatcgc
     7201 agtactcatt ttatctgtat ttgaaagagg aacatataga tcgactaagt ctcccataac
     7261 atcaactgaa tatgtaaaac cttttgaata tattcctctt tcaggcatac cggtttcaaa
     7321 tcctatagtt ccggcaggaa tttcaagctc ttcatcttcg tcaggcgttt cctcatcttc
     7381 atccccggcc gtgtcgccgg tatttcctgt gttcccgcta tcacctgagt caccagtatt
     7441 tccagtgttt ccggtattgc ctgtatcgcc attattctct atctcatcaa catctgagag
     7501 ctcattaatt ttatcactca cggattcatc aacatctacc gcttcatctg tcgattcatc
     7561 attgttttcg gaatcctgac tcttttgatc tttatcaccg caggacaaca agaacaatga
     7621 aataaacagt aatattaaga actttttcat ttagacctcg aatgttaaaa aatgaaactg
     7681 cctggaaatt acttctgaac tgtataaatg atcagcttgt tactttcgta aagagttaca
     7741 atcaactcac tctgaccatc atcatcaaga tcaactatct tcattccgcc tgcctgaccg
     7801 aactgtgaat tcggagcttc gggctgtgat cctactggct ctccgccaat tacatgctga
     7861 gtgaactcac ctgcggaagt ctgctgatac cagaaaattc ttgcatcacc gtcacctgaa
     7921 agagcgatgt ccatcaaacc attacctgtc agatcgccta taccgaaaac acctggagcc
     7981 gccatcggtg ccatactgct tccctttctt gactttatgc cgtcagagat aagctttttt
     8041 gtccacggct gtgtcgggtc ttcaggaata tcaaacacaa aaagcccttc cattgcgctg
     8101 gatgaatttg cagcagtatt agtatgattt gttcctactg cgcgacggac accgtcgccg
     8161 taaagatcgt caactatgtt tatctggaac ccttcaccgc tgtcattgtt gatagcatgc
     8221 ggagtccagt tgtcaattgc aactctttca agccagacaa acgatggctg tgcttcggga
     8281 tcaatgaaat attgagcagc tactatatcc aagtctccat cttcatcaat atctataact
     8341 cttgaaaaag ggccgccgtg actttctgaa atgaatttct ttgttgtatc aaaccttatt
     8401 cctgaatcag tccctttaaa ccataaaagt ttttcttcac gcgctgtaat tgagcccatc
     8461 atgttctttg tgaacttctc tgctgttgta acaatatctt taattccgtc accgtcaaaa
     8521 tcaacatatt ctattccgaa atagaatgaa ggatccccca tcggcacaat atcatgtctt
     8581 tcccaagttc cgcctttgtt ttccatccag aagatcgttc cacagggacc gccaagagga
     8641 tttgcttcac aggcaaggaa tccgcctgga attatcagat caagatccgt atcaccgtca
     8701 acatccgcaa caacaggagg atgcggaaac tttacagcag ttcctgtgtc aagctgctgt
     8761 ttattccaag tcgcaatatt tcctgttccg taatatatct ggatctttcc ttccatactt
     8821 gtgtttgaaa aaccggatgg accgtattcg cacgatataa tatcaagatt tccgtcaaca
     8881 tccagatctg caactgttac aaaagcagga ttgtaggggt tatcgtcaat tacattttca
     8941 acaagtgtta tgtttactgc cgtgtcacct gaatttcctg tatttcctgt gtttcccgta
     9001 tcacctgtat ctccagtgtt gcctgtgttc cctgtatcgc ctgtatttcc tgtatttcct
     9061 gtatcgccgg tatttccagt gtttcctgta tttcctgtat caccggtatc gccggtattt
     9121 ccagtgtttc cggaatcccc ggaatc
//
LOCUS       DAOOYB010000109         8901 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_8645_length_8901_cov_12.273344, whole genome shotgun sequence.
ACCESSION   DAOOYB010000109 DAOOYB010000000
VERSION     DAOOYB010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8901
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8645_length_8901_cov_12.273344"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            168..1538
                     /locus_tag="PL056_07655"
     CDS             168..1538
                     /locus_tag="PL056_07655"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM46930.1"
                     /translation="MMQEKIKKLIAQGENESVEFKESFSEGLIISLVAFANRSGGTVI
                     VGVDDKGTISGTNTDKESIQKWINEIKTKTYPNIIPDVEEINVSGRKTVVFTIKEYPI
                     KPVAYKGRYYKRMANSNHLLDISEAADMHLSIFNSSWDFHFDSTHKLEDISLEKVIRY
                     MKRMESQNITVSEDPLHFLIKRNLMREGKITHAAYLMFKKKDSFITTIELGRFQTSTI
                     IKDSARSKSDIVTQVEEVVDFVKKHINKEIIITGDPQNRQVWQYPLEAIREIVLNMII
                     HRDYRSSADSIVKVFDNKIQFFNPGKLPEGITVENLKENKYISNPRNKLVADCFKDMG
                     FIEKYGSGIGRVIHYFKEAELPLPEFTNISDGFMVTIFSKEENVTENVTENVTENVTE
                     KRWRKIIEIIRENKVISVDRIALMCKVDRRTILRDMENLKEKRIIKRIGPNKGGHWEI
                     IENENL"
     gene            1525..1782
                     /locus_tag="PL056_07660"
     CDS             1525..1782
                     /locus_tag="PL056_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46931.1"
                     /translation="MKIYKTLGSKQTLLDKGQASLMAVFFFIGAIYENLFFLLFAPTI
                     VFAVTFIIKEKYFSGKPEWGIYVLRMLKGSRIYYTRHIKDE"
     gene            1775..4120
                     /locus_tag="PL056_07665"
     CDS             1775..4120
                     /locus_tag="PL056_07665"
                     /inference="COORDINATES: protein motif:HMM:NF040179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM46932.1"
                     /translation="MNNIFKMLRIASIESDLIITSSMQRYSIWETDGIDCYFREPDLT
                     RAYSAFMREMKENTTVEFFQISKLKDDTVERRLKEEKDIVTKQRLNYIRKAGIKKVRN
                     FFLVSDDLPKKELSKDPVNIQYHKNMIGSFGLKFKRVSDDEIREIMYNIISFDSNLKV
                     HPEMTIREALIQRQCSAGPEYFKVGDTYCKVLSLKHLPDATRPFMLSYLLDYLLYDYV
                     FAISLNILPQGKEFVSLEAKERFYIATSGRGAARNANEADELMTLLAESRHKIGYLSA
                     KLIVWNKDLKQLEKSAIELSNIMKNENCFFEEETWGHDLEFFKSLPSQLSYSERQHRV
                     LSPNFIDMIPASKHGHADSQGKYPLFLRNRFGEVYGFDAGSSKRNNKNGSIFGCSGSG
                     KSVTVNALIAHTFFPNIREDKSHPGRIFIVDFAGAENSSYLKMADLYGGVFIPVDSKG
                     RVVINPFPERSKIFADGKWDSSMLNFLQICMDLILEVREKSMKADLYRIIISKAIKEM
                     YQQIERPVLSDLVRFINDDDREKCDTLKKLLNGFIDDPVSRIINGYSTIDYGNDPFVI
                     YDLQGITGLNDKLKELLTFIVIQEAKKTAFEITNSFIMFDEAAQLIKDPRLGDLIEEL
                     FATARKYNTGIWTITQNFLSFKEVALSSKIKINTTTTIFLSHANDEEAKRLVASDFGF
                     TETERGAFDSLRTVKGEYALALFRTQISDTVESEVVRIELSPFDYAVATSDKEDNRLL
                     KKYAEKRKLKLIDACAEAAAFAGRNNLFVIDAVKKLIEQAQ"
     gene            4117..5007
                     /locus_tag="PL056_07670"
     CDS             4117..5007
                     /locus_tag="PL056_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46933.1"
                     /translation="MNINIYYNPKTLLQQAGAGWVWDVTFLIILIGLTISFVVEVFKI
                     QKKGNPDFSRVVWKTVIIVLMYTSLPGLIEKTMNFVNSTITSSELDNEFYKAFSLYSA
                     NLSGTGKKSEINPETCPSNFDATLVQAGFQLISSYYFQYFAKLFVFILLVSVWVVKEL
                     IFSWAWPVMMSINMIGLCAALTIPAFPSKGFGSVGSFFKSVAVFTLWPVIYSVFIVIT
                     KDALIATFKLAQSCIVCPEVFEVGKNTIVSISGILFFALGIALVPFISSKIVNSPEVN
                     TIVANIKSSPIIEIVSKRVK"
     gene            5009..5761
                     /locus_tag="PL056_07675"
     CDS             5009..5761
                     /locus_tag="PL056_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46934.1"
                     /translation="MTKQNKLTDADMFFSAMKFKDVQLLRKTYSFFMFLALIFMFVLA
                     TVSILFAFTVSMKPTPVIAFDAYGKKAVFSPDDTVESETSKVRIHRFMTEFVNKFEGV
                     SPNIEEDLKDAYNMLTPKFRQILLDKAVHKEKIETWKNKNFETQFIIKNLKILKGSFE
                     HGSSLTLEGIGEMTFRNVINYSGENEQKKDFVYFSALLIVTPVSFELSPDGLFVEFYH
                     GKSLGDFRSLRAHLLENKKEYLIEGENKEVFE"
     gene            5758..6702
                     /locus_tag="PL056_07680"
     CDS             5758..6702
                     /locus_tag="PL056_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46935.1"
                     /translation="MIFKILLIIFSFFTVMADDTIEVKAKDPRFIPITEKVAVITLKN
                     SDTVIYRTVNIAIGEVFVLEFPKNIILEETPTIGDAGLLKIEVENDPLKMRIWGLMFP
                     DADESSMYGLNSNIQFKINSGTTFIFNMVLQPAANASNRLIFSYPEWENAQKDINRQL
                     ETLKKDLMNEHGKKIKSLEKDSEKMMIQFMAKSFSEFYQCNNYSARVEENLVFLRSDR
                     ICKIGRDGLITVNFSIKNRYRQRFHIESVKVYSMKGGKVEIDNAQYHIDRFSMQFDEV
                     VNGALAFKIKDEDYSTEYTVEVIESAGKKRKLELKVSF"
     gene            6703..7752
                     /locus_tag="PL056_07685"
     CDS             6703..7752
                     /locus_tag="PL056_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46936.1"
                     /translation="MSEKNKENNALNILLDETENIGSECDNHTAEEKSVYRHNRKKNG
                     IVILFSIMFSALLGAGIALSFVNKEKSIEESAPEINNFDEFKRGRISGSLRSAMNTNE
                     KKEDKLELPAVKTDTEKVRINNYQGGKNRIKDEEILEKYATAVDPANSENIAGTRSGR
                     RHFVPGGSSSQGGVFIKGGSKNKENGKTLDISDIKIKVRLEFSIRSTAASTIVAVTTE
                     DHDRIPKGSKFYGSASGYVNKRTQIMFNKLIVNGVEFSIKGFAISGRDPGIESEVTDI
                     AKENIDSSVKQGIAQTAANVVAKYAGVAGNVTGEAASNTVDPASAEIKKQQEANKMTQ
                     EYRVPAGTSFFIYLE"
     gene            7755..8375
                     /locus_tag="PL056_07690"
     CDS             7755..8375
                     /locus_tag="PL056_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46937.1"
                     /translation="MGIIKIFFALIIFFSFTISAEEGEIQINIGGGGFFPLSLKTDDR
                     TVVVYSSWNVGLNTYFGLSDNFDIGIQPSFTRLKDTSRNSEFMELGGREYFNYWRFQM
                     LALLRYNLYPGSFFSPHIIAGGGFKVETYTDWDFFTTTGKRIAGYKKDNYASINGVVA
                     GGIDLQFRVWEWFILSTQVLYKWSPGDHSIDLLGFLGATFFVNFYR"
     gene            8385..>8901
                     /locus_tag="PL056_07695"
     CDS             8385..>8901
                     /locus_tag="PL056_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46938.1"
                     /translation="MKKFFLTALIPVVLFSCDTKRPDVENLEYSLTVNSIQNGANVEV
                     LDGETVVVSGTTDESGAVTFSGLNAVKTLTVKVCKGTATLVSSADPVAWTGCNETEFT
                     PEDKQDVVVTVDFLSTFISKYDSETSTEEWMDYLGLTTLTSPTLQTSLTDSTKRYLWY
                     QGIAKIAENVSK"
BASE COUNT         2944 a         1644 c         1857 g         2456 t
ORIGIN      
        1 catatctctt cttttcagat atataaacaa ggatcaaaac atctggagcg atgctttaaa
       61 aattcttgga gcaggtgcag gacttgccgc attctcgtca attttaaaaa ttattgccgg
      121 atggatttag cagaatgccg cgaatggtga atcgtgaaaa aacacacatg atgcaagaaa
      181 aaattaaaaa actcattgct caaggtgaaa atgaatctgt tgagtttaag gagtcattct
      241 ctgaaggatt gataatttct cttgtggcat ttgcaaacag atcaggtggt actgtaattg
      301 ttggtgtaga tgataaagga accatttccg gaacaaacac cgataaagaa tcaatacaaa
      361 aatggataaa tgagataaag acaaaaactt atcccaatat tattcctgat gtagaggaga
      421 taaatgtctc tggcagaaaa acagttgtct ttacaataaa ggaatatccg ataaaaccag
      481 ttgcatacaa aggccgatac tataaaagaa tggcaaactc taatcatctt cttgatattt
      541 ccgaagctgc agacatgcat ttgtcgattt ttaattcaag ctgggatttt cattttgact
      601 ccacacacaa attggaagat atttctctgg aaaaagtaat aagatatatg aaaagaatgg
      661 aatctcaaaa tattactgtt tctgaagatc cacttcattt tctgataaaa cgcaatctga
      721 tgagggaagg aaaaataact catgcagcat atcttatgtt caagaagaaa gattcgttta
      781 ttacaactat tgagctcggc agatttcaga caagtacaat aataaaagat agcgcgagat
      841 ccaaatcaga catcgtaact caggttgaag aagttgttga ttttgtcaaa aaacatataa
      901 acaaggaaat cataattact ggagaccctc aaaacagaca ggtctggcag tatccacttg
      961 aggcaattcg agaaatcgtc ttgaatatga tcattcaccg tgattaccgt tcttctgctg
     1021 attcaatagt taaagtgttt gacaacaaaa ttcaattctt taatccaggc aaactgccgg
     1081 aaggaatcac agtcgagaac cttaaagaaa acaagtacat atcaaatccc agaaacaagc
     1141 tggttgcgga ttgttttaaa gatatggggt ttatagaaaa atacggttcg ggaatcggaa
     1201 gagttatcca ttattttaaa gaagcagaac tgccgcttcc tgagttcaca aatatttctg
     1261 atggttttat ggtaacaatt ttttctaaag aagaaaatgt cactgaaaat gtcactgaaa
     1321 atgtcactga aaatgtcacc gaaaaaagat ggcgtaagat tattgaaata ataagagaaa
     1381 acaaagttat ttctgtcgat agaattgcat taatgtgcaa agttgacagg aggacaatac
     1441 ttcgtgatat ggaaaatctt aaggaaaaaa gaattataaa acgaataggg ccgaacaaag
     1501 gcggtcactg ggaaataatt gaaaatgaaa atctataaaa ccctcggcag caaacaaacc
     1561 cttcttgaca agggacaggc atcccttatg gcagttttct tttttatcgg agcgatatat
     1621 gagaatctgt tttttctcct ttttgccccc acgatcgtgt ttgcggtcac attcataatc
     1681 aaggaaaagt atttctcagg aaaaccggag tggggcattt acgtgctccg catgttaaag
     1741 ggcagcagaa tctattacac taggcatata aaagatgaat aatattttta aaatgttgcg
     1801 catagcctca atcgaatccg acctcatcat cacttcttcg atgcagcgct attccatatg
     1861 ggaaaccgac ggaattgact gttatttccg tgagcccgac ctgacaagag catattcagc
     1921 gttcatgcgg gaaatgaagg agaacaccac cgttgaattc tttcagatat caaaactgaa
     1981 agacgataca gttgaaaggc gcttaaaaga agagaaggac attgtcacaa aacagcggct
     2041 taactacatc cggaaggccg gaataaagaa agtccgtaat ttttttcttg tcagtgatga
     2101 tctccccaaa aaagaacttt caaaggaccc ggtgaacatc cagtatcata aaaacatgat
     2161 cggttcattc ggtttaaagt ttaaacgggt gagtgatgat gaaataaggg aaataatgta
     2221 taatatcata tcatttgatt caaatctgaa agttcatcct gaaatgacca tcagggaagc
     2281 actaattcag agacagtgtt ccgccggccc tgaatatttt aaggtgggcg acacctactg
     2341 caaagtgctc tcactcaaac accttcccga tgcgacccgt ccgtttatgc tgagctatct
     2401 ccttgattat ctgctttacg actatgtttt tgcaatatcc ttaaacattc ttccgcaggg
     2461 caaagaattt gtatcgttag aagcaaagga aaggttctac attgcaacta gcggcagagg
     2521 agccgcaagg aacgcaaatg aagccgatga actgatgaca ctgcttgcgg aatcccgcca
     2581 taagatcgga tatctttctg caaagctgat cgtctggaat aaggacttaa aacagcttga
     2641 aaaaagtgca atagaacttt caaacataat gaaaaatgag aactgctttt ttgaggaaga
     2701 aacatgggga cacgaccttg aattctttaa atcgcttcca agccagttat cttattcaga
     2761 aagacagcac cgggtgttat cgccaaactt cattgatatg atacctgctt caaaacacgg
     2821 tcatgcagac tctcagggta aatatccgct gttcctccgt aacagattcg gtgaagttta
     2881 cggatttgat gccggaagtt caaaaagaaa caataagaac ggctcgatat ttggatgttc
     2941 aggaagtgga aaatccgtta cggtgaatgc gttgatcgct cacactttct ttccaaacat
     3001 tagagaggat aagtcacacc ccggacgaat tttcattgtg gatttcgcag gggctgaaaa
     3061 ctcttcatat cttaaaatgg ctgaccttta cggcggagta ttcattcccg ttgattcaaa
     3121 agggagagtt gtcataaatc cgttccctga aagatcaaag atatttgcag acggcaagtg
     3181 ggattcatca atgctcaatt ttttgcagat ctgcatggat ctcatccttg aagtcaggga
     3241 aaagagcatg aaagcagacc tttacagaat aatcatatcc aaagccatta aggaaatgta
     3301 tcaacagatt gaaagaccgg tgttaagcga ccttgtgagg ttcattaatg atgatgacag
     3361 ggagaaatgc gacacactta aaaaactcct caatggtttt attgatgatc cggtgtcacg
     3421 gataataaac ggttactcaa ctattgatta tggaaacgac ccttttgtaa tatacgacct
     3481 tcagggaata accggcttga atgataaact taaggagctt ctcactttca tagttattca
     3541 ggaagccaaa aagaccgcat ttgagataac caacagtttt atcatgttcg atgaagcggc
     3601 tcagctcatt aaagacccac ggctcggcga tctcattgaa gaactttttg caacagccag
     3661 aaaatataac accggcatct ggacgatcac ccagaatttt ttgagcttta aagaggttgc
     3721 tctatcaagc aagatcaaga ttaatacaac tacgacaatt ttcctttctc acgccaacga
     3781 tgaagaagca aaacggcttg tagcaagtga tttcgggttc actgaaactg aaagaggggc
     3841 atttgattca ctccgtactg tcaaggggga atacgccctt gcccttttca ggactcagat
     3901 atctgacacc gttgaatcgg aagtggtgag aattgagctt tcaccctttg attatgcggt
     3961 cgctacaagt gacaaggaag acaacagact tttgaagaaa tatgctgaaa agagaaaact
     4021 gaaacttatt gatgcatgtg ctgaggcagc ggcttttgcc ggtagaaaca atctgtttgt
     4081 catagatgcc gttaaaaaac ttatcgagca agcacaatga acataaatat ctactacaac
     4141 cctaaaactc ttttacagca agcaggggcc ggctgggttt gggatgtaac atttctcatc
     4201 atactcatcg ggcttacgat aagttttgtc gttgaagtgt tcaagataca gaaaaagggg
     4261 aaccccgact tttcaagggt ggtttggaaa accgtgataa tagttcttat gtacacctct
     4321 ttacccggac taattgaaaa aacaatgaac tttgtaaatt ccacaattac ttcaagtgag
     4381 ctggacaatg aattttacaa agcgttttcg ctttacagtg caaatctcag cggcacagga
     4441 aaaaagtcgg aaataaaccc tgaaacctgt ccgtctaatt ttgatgcaac cctcgttcaa
     4501 gcagggtttc agctcataag ttcatattat tttcaatatt ttgcaaaact gttcgttttt
     4561 attctgctag tttcagtatg ggttgttaaa gaactcattt tttcgtgggc ctggccggtt
     4621 atgatgtcaa ttaacatgat cgggctctgt gcggcactta ccattcccgc ttttccatca
     4681 aaagggttcg gaagtgtcgg cagttttttt aaatctgttg cggtttttac tctctggcct
     4741 gtcatatatt cagtgttcat agttataaca aaagatgcat tgatagctac atttaaactt
     4801 gctcagtcgt gcatagtgtg tcccgaagtc tttgaagtag gaaaaaacac catcgtttca
     4861 atatctggaa ttttattctt tgctcttggc atagcacttg ttcctttcat ttcttcaaaa
     4921 atagtgaatt ctccggaagt aaacacaata gtggcaaaca ttaaatcttc tccaataatt
     4981 gaaatagttt caaaaagggt gaaataacat gacaaaacaa aacaaactga ccgatgcgga
     5041 tatgttcttt tcagctatga aatttaaaga tgttcaactt ttaagaaaaa cttattcatt
     5101 ttttatgttt cttgccctta tattcatgtt tgtgctcgca actgtttcaa ttctttttgc
     5161 gtttactgta tcaatgaaac cgactccggt aatagctttt gacgcatacg ggaagaaagc
     5221 tgttttttct cctgatgaca ctgttgaaag tgaaacatca aaagtgagaa ttcacaggtt
     5281 catgaccgaa tttgttaata aatttgaagg tgtcagtccc aacattgaag aagatctcaa
     5341 agatgcctac aatatgctca cccccaaatt caggcagata cttcttgata aagcagttca
     5401 caaggaaaag atagaaacct ggaaaaacaa aaactttgaa actcaattta taataaaaaa
     5461 tcttaagatc ttaaaaggaa gtttcgagca cgggtcatca cttacacttg aagggatagg
     5521 tgagatgact ttcagaaatg tgataaacta ttccggagaa aacgaacaga aaaaggattt
     5581 cgtctatttt tcagcacttc tcattgttac acctgtttct tttgaacttt ctcccgatgg
     5641 actttttgtc gaattttacc acggtaaaag tcttggcgac ttcagaagct taagagcaca
     5701 tctacttgaa aacaagaaag aatatttaat tgaaggcgaa aacaaagagg tgtttgaatg
     5761 atatttaaaa ttcttttaat aattttcagt ttttttacag ttatggccga tgacactatt
     5821 gaggtcaagg caaaagatcc ccgtttcatc ccgataacgg agaaagttgc ggtaattaca
     5881 ttgaagaaca gtgacaccgt catttacaga acggtcaaca tcgcaatagg tgaagtcttt
     5941 gtacttgaat ttccgaaaaa cataatactt gaggaaaccc cgaccatcgg tgatgcggga
     6001 ctcctgaaga tcgaagtaga gaacgatccc cttaaaatga gaatatgggg cctgatgttc
     6061 cccgatgccg atgaatcatc aatgtacgga ctcaactcaa acatccagtt caagatcaat
     6121 tcaggcacta ctttcatatt caacatggtt cttcaaccgg cggcaaatgc aagcaaccgg
     6181 ctaattttca gctatcctga atgggagaat gcacagaaag acataaacag acagcttgag
     6241 acacttaaga aagatctgat gaacgagcac ggaaagaaga taaagagtct ggagaaagac
     6301 tcagaaaaga tgatgataca gttcatggcg aagtcatttt cagagtttta ccagtgcaac
     6361 aactactcgg caagggttga agagaatctt gtattcttaa gaagtgaccg gatatgcaaa
     6421 ataggcaggg acgggctcat cacagtgaac ttttcgataa agaaccgcta ccgtcagcgg
     6481 tttcacattg aaagtgtcaa ggtctattca atgaaagggg gcaaggtcga gatagataac
     6541 gcccagtatc acattgaccg gttctcaatg cagtttgatg aagttgtgaa cggagctctg
     6601 gcatttaaaa taaaggacga ggactattca actgaatata ccgttgaggt tattgaaagt
     6661 gccggaaaga aaagaaaact tgaactgaag gtgtcatttt aaatgagtga aaaaaacaaa
     6721 gagaataatg cactgaacat tcttcttgat gaaacggaaa atatcggaag cgaatgtgat
     6781 aaccatactg ctgaagagaa gagtgtatac cggcacaaca ggaaaaagaa cggtatagtt
     6841 atcctttttt caattatgtt ttcagctctt cttggtgccg gaatcgccct atcttttgtc
     6901 aataaagaaa aaagtattga ggaaagtgct cctgaaatta ataatttcga tgaatttaaa
     6961 aggggaagaa tttcaggaag cttgaggtca gcaatgaata caaatgaaaa aaaggaagac
     7021 aaactcgaat tgccggctgt taaaactgat acggaaaaag taagaattaa taattatcaa
     7081 ggtggcaaga acagaatcaa agacgaagag atcttagaaa aatatgcaac tgctgttgac
     7141 cctgcaaatt ctgaaaacat cgctggaaca agatcaggga gaaggcattt tgttccagga
     7201 ggatcttctt cgcagggagg agtgttcata aaagggggga gcaaaaataa agagaacgga
     7261 aagacacttg acataagcga cataaaaata aaagtgcgtc ttgaattctc tatccggtca
     7321 actgccgctt cgaccatagt tgcagttaca acggaagatc atgacagaat acccaagggt
     7381 tcaaaattct atggctctgc atcgggatat gtgaataaaa ggactcagat aatgtttaat
     7441 aaacttattg tgaatggcgt tgagttctcc ataaagggtt ttgcaatttc cggtcgtgat
     7501 cccggaatcg aatccgaggt aactgacatc gctaaggaaa acattgattc ctcggtaaaa
     7561 caggggattg cccagactgc cgccaatgtt gttgcaaagt atgcaggggt tgcgggaaat
     7621 gtcacaggtg aagccgcttc aaatacagtt gaccccgcca gtgcagaaat aaaaaaacag
     7681 caggaagcca ataaaatgac gcaagaatac agagtaccag ccggaacaag cttttttatt
     7741 tatttggagt aattatggga attataaaga tcttttttgc tctgatcatc tttttttcct
     7801 tcaccatttc agcagaagaa ggagagatcc agataaacat aggaggcggc ggtttctttc
     7861 cactctctct taaaaccgat gaccgtacag tcgttgtcta ttcaagctgg aatgtgggat
     7921 taaatactta tttcggtctc agcgataatt ttgacatcgg aattcaaccc tcattcaccc
     7981 ggcttaagga tacttcaaga aattcggaat ttatggaact tggcggtcgt gaatatttca
     8041 actactggcg tttccagatg ctggccctgc tcagatacaa tctttatccc ggctcattct
     8101 tttctcctca tattattgca gggggcggct tcaaggtcga aacttacacc gactgggatt
     8161 ttttcacaac gaccgggaaa agaatagccg gctacaaaaa agacaattat gcttccatca
     8221 acggtgttgt tgcaggtggc attgacctcc agttcagggt gtgggaatgg ttcatactgt
     8281 caactcaagt gctttataaa tggtctccag gagatcattc aattgacctg ctcggttttc
     8341 taggagcaac tttttttgtg aatttttaca gatagggggc aaaaatgaaa aagttttttt
     8401 taactgcact tattccagtt gttttgtttt cgtgtgacac gaaaagacct gatgtggaaa
     8461 accttgaata ttctttgact gtcaactcta tacaaaatgg tgcaaatgta gaagttcttg
     8521 acggggaaac cgttgttgtt tcagggacaa ctgatgaatc aggcgctgtg actttcagtg
     8581 gattgaatgc ggttaaaact ttaacagtga aagtgtgtaa aggaacggca acccttgttt
     8641 caagtgctga tcccgttgca tggactggtt gcaatgaaac ggagttcact ccggaagata
     8701 agcaggacgt tgttgtaaca gttgattttt taagcacatt catttcaaaa tatgattctg
     8761 aaacatcaac tgaagaatgg atggattatt tggggctgac aactctgacc agtccgacac
     8821 ttcagacttc gctgaccgac tctacaaaac ggtatctctg gtatcaggga atcgccaaga
     8881 tcgctgagaa tgtatcaaag g
//
LOCUS       DAOOYB010000110         8834 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_8742_length_8834_cov_8.235904, whole genome shotgun sequence.
ACCESSION   DAOOYB010000110 DAOOYB010000000
VERSION     DAOOYB010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8834
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8742_length_8834_cov_8.235904"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="PL056_07700"
     CDS             complement(<1..507)
                     /locus_tag="PL056_07700"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HPM46939.1"
                     /translation="MEFFDIKGGDVIRGEITLSGNKNEALPVIAAALLARGRVKIDNV
                     PDILDVKNMLQIAEDIGVTVTRLSQNCIEMDASNIISKPLDKELCGLVRTSILFAGPL
                     LARNGSVILPPPGGDVIGRRRLDTHFTGFEQLGATYSHDSNGYNLSANRLKGADIFLD
                     EASVTATEN"
     gene            639..714
                     /locus_tag="PL056_07705"
     tRNA            639..714
                     /locus_tag="PL056_07705"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:672..674,aa:Ile,seq:cat)
     gene            832..1566
                     /locus_tag="PL056_07710"
     CDS             832..1566
                     /locus_tag="PL056_07710"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HPM46940.1"
                     /translation="MLDELKKLKDLQFIDSQVFDLEEELDSLPEQNELLNAKITEMEG
                     KLKELRSAIAIQLEEKQKRDDILKKGEDKLKTITGKQSAIRNKDEYNALLREIDNIKR
                     FNRDLSDEIAEIDKEIEFKNNELKLIEEETVKKIEENRKKMSENEKRVKELDNTLEKI
                     SEKREKLIQNIRPAVYRKYQRIFESSPNGKAIALAEDRVCHGCNMTLPPELFNMVLRA
                     TRIEVCPNCQCILIPVKKENKETPEQ"
     gene            1585..1936
                     /gene="rnpB"
                     /locus_tag="PL056_07715"
     ncRNA           1585..1936
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PL056_07715"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1937..3565)
                     /locus_tag="PL056_07720"
     CDS             complement(1937..3565)
                     /locus_tag="PL056_07720"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPM46941.1"
                     /translation="MKKFLISLTILIFFAGCSDDGRVKSDLEESVDDAVYEESDDIPD
                     DDPVDSENDEDSTEKFSFDIQRVYNDVAWFAHADRTGRQPGTQGNADSVEYVRALFDE
                     LGLMPAGDGETYLQSFSFDQWVITGTPEAEIDGTLLSTETGFQVMQYSGRGDVSAEVV
                     FAGHGITVPPFKKSEYPDCPLPETGYDDFAGIDVTGKIALVIRRGPGNLESVHNSCPA
                     NDACVAAPCLWNFGYKSKNAAIHGAAGVIVVNHYQESGAVPAGMTLGGEYFVEDLPVI
                     FADRDTMEETIPDMKSWSGSIDADLVPQSSETGINAEIKVETEIETVRADNVIGVLPG
                     TDQELSEEVIVIGAHVDHLGKDPVSGGIYYGADDNASGTAVVMELARAFVLGGHKTAR
                     TVVFAAWNAEELGLIGSCEHVENPAFPISQTVAAWSIDMVGAGDGSGLAVYGATLSSN
                     RWLADVMSGYADKLELDLEINNAQPLDASDHVCFYYAGVPAVLLSTLGTHSYYHTVND
                     TIETINKSDLEAAVWLSWSGIFPIAMGIEDDYLQ"
     gene            complement(3584..4939)
                     /locus_tag="PL056_07725"
     CDS             complement(3584..4939)
                     /locus_tag="PL056_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46942.1"
                     /translation="MKLFERYLVVFSVLFTGITFISCNVDVKKIVPKNDETAAADDDL
                     SDDLSDDLSDDLSDDLSDECSTEPDEDSDTPQICGNNMVDKGEVCDGNVVNCVDIDSS
                     KFIGGKAKCLDDCSGFNTITCSEKEDNGDSGNSGDSGNSGNTGNTGDTGDTGDTGNTG
                     NSGDSGDSGNTGDTGNSGDTGNTGNTGDTGNTGNTGDTGNTGNSGDTGNTGDTGNSGC
                     IGNGLDEDGDGIDDNCDNCPASFNSGQKNTQNDGIGDVCEAPFNYDLLSQITVFEPFI
                     DQQPSWSPTGGTWNYGSSEMSISTSPYGQNNYYSYQIPNGPFSVEVTFRYDEGDLGGN
                     SYAELLFSVQQVSNDTHWYTCLFRRDNNELAIWRYAGGSSIGFVKGATATAPATDDQI
                     HKVRVFYNGSEIRCSFMDEAGGTGEVVISGGDVQQDMSGRAGLRVYNERTVYTSFVVY
                     Q"
     gene            5099..6715
                     /locus_tag="PL056_07730"
     CDS             5099..6715
                     /locus_tag="PL056_07730"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HPM46943.1"
                     /translation="MKKSIVILFLGLTFFFVSCGENKKANDESGTPDEEVSDAEVDED
                     GLTNDEDAADESFSPFIDALKKDLEKSDALGVSVAVMKGGNVVFAHAFGFKDAGRKVP
                     LTPETLMQIGSTTKQMTATALLQKVEEEKVALENTINDVLPDLEFKLDETWDDSITVH
                     HLLSHQGGFYDWIPWDFMPEDDMLEGIGSTYAKKYFLMNPPGAFYNYSNPNFAFAGLI
                     TETLDNRFWPDIMREDIFLPLGMERTYLRKSEVKKDGDFSDSYGYDYNEIESAELTDI
                     TIDKIADSAFTRPAGLVWTTPLQMMKWADFIMNGNNEVLGDKYRKMITTPHVDTLYGA
                     GTMHYGYGMMVETGYMDKAGKWYEIPVWEHGGNTISFSNILYILPDQDFAVSICSSGY
                     GTDFTESLDVAITTLADLPEPSETVPKYETDPEKFDDHVGTYNDAYNVGTMIITRDGD
                     KLLVSAPTLEGYNYEVGPELIPVSSDIFFLYLDGQPLDITFVKEEGHSKYMRNRIFVL
                     TKVEEEETLITPTSQQIENFILRSKRKLLP"
     gene            6825..7856
                     /locus_tag="PL056_07735"
     CDS             6825..7856
                     /locus_tag="PL056_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HPM46944.1"
                     /translation="MEKHEIESARGQFLVYQAEDGKLKLDVRFEDESVWLTQQLMADL
                     FQTSKQNVSHHINSIYEERELLPEATVKKYLTVRSEGNREVKRLLDYYNLDMIISVGY
                     RVKSHVATRFRIWATQKLTEFIKKGFVLDDERLKNPDLPFDYFEELEKRIQDIRTSER
                     RFYQKITDIYATSIDYDPTLDISLEFFKTVQNKMHWAITGQTAAEIIHSRADANKPKM
                     GLTNYRGSKVRKQDVTIAKNYMNEKELAELNNLVEQYLIFAQGQAMRRIPMYMSDWIK
                     KLDAFLNINDRKILDHAGKISHELAKQLAESEYEKYHVKQLQTFSKELSDFDKAVKQI
                     ESSKKEIKK"
     gene            7951..>8834
                     /locus_tag="PL056_07740"
     CDS             7951..>8834
                     /locus_tag="PL056_07740"
                     /inference="COORDINATES: protein motif:HMM:NF025388.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4238 domain-containing protein"
                     /protein_id="HPM46945.1"
                     /translation="MNTPKRHHYLPELYLKRFCTNEQLFIYDRKKNEIRPQTPVNTGV
                     QFHYYSSINEKGEKKPEIETDLLSRIDNDANNVIEKILKNEQINEFDKEKLSIFLGFM
                     VHRVPYFENSFNSMYSYINKKLLNSIFCSEELVQIMTADNEENITAEEFVDFYKNSNL
                     KITPHRNESLRFMLSQSLVASECFMKYKWEILYCSSESSFITSDNPVITTKFVEQSTG
                     ENLGNLILFPLSNNLCLSMFHKGTEVVFNEINQSSVEKINAVIATNSYQYILGKDKTI
                     IEKIVAENKIQGINNKKM"
BASE COUNT         2778 a         1866 c         1761 g         2429 t
ORIGIN      
        1 attctctgtc gccgtaacac ttgcttcatc aagaaatata tctgccccct taagacggtt
       61 tgcagaaaga ttatagccgt tgctgtcatg gctgtatgtt gccccgagct gctcaaaacc
      121 ggtaaaatga gtgtcaagcc ttcttctccc tatcacatcg cctccgggag gtggaagtat
      181 gacggatccg ttccttgcaa ggagtggtcc cgcaaaaaga attgatgttc tgaccagtcc
      241 gcaaagctct ttgtcaagcg gttttgaaat tatgttcgaa gcatccattt cgatacagtt
      301 ctgtgaaagt cttgtaactg ttacaccgat atcttcagct atctggagca tgttttttac
      361 atccagaatg tccggaacat tatctatttt aactctgccg cgggcaagaa gggctgccgc
      421 aataacagga agtgcctcat ttttattacc tgaaagagtt atctcccctc ttattacatc
      481 tccgccttta atatcgaaaa attccatgaa accctctttt gcaaagtgtt gaaacggata
      541 acacaggcgg taatttacag caaaatccat ttttttccaa tttttttgtt taaaaatttg
      601 caattttaat aaatgcgatt ataaaggttg cgtctaacgg gcctatagct cagttggtag
      661 agctcccggc tcataaccgg atggtcagag gttcgactcc tcttgggccc accaccttat
      721 tttgtggagg gtagactttg cggtttattt acattaccat gaacataaat tcaataagca
      781 cttgtgaaaa caagtgcttt ttttttgcat aacaaatgat ggaggctagt gttgcttgac
      841 gaactgaaaa aacttaaaga tctccagttt attgattctc aggttttcga tcttgaagaa
      901 gaacttgatt ctctgcctga acaaaatgag ctgttgaatg ccaaaatcac ggaaatggag
      961 ggcaaactca aggaattgag aagcgccata gccatccagc ttgaagaaaa acagaaaagg
     1021 gatgacattc tcaaaaaagg cgaggacaaa ctcaagacga taaccggaaa acagtcggct
     1081 atcagaaaca aggatgaata caacgccctt ttaagagaga ttgacaacat taaacggttc
     1141 aaccgtgatt tgagcgatga gattgctgag atcgataaag agatagagtt caaaaataat
     1201 gaacttaagc tcatcgaaga ggaaaccgta aaaaagatcg aagaaaacag aaaaaagatg
     1261 tcggaaaacg aaaaaagggt taaagagctt gataacactc ttgaaaagat ctctgaaaaa
     1321 agagagaaac ttattcagaa catcagacct gcagtttacc gtaaatatca gagaatattc
     1381 gaaagttctc ccaatggaaa agcgatagct ctggctgagg acagagtctg tcacggttgc
     1441 aatatgacac ttccacctga actcttcaac atggtgctcc gggccacaag aatagaagtg
     1501 tgtccgaact gtcagtgcat tctaattcct gttaagaaag agaacaagga aactcctgaa
     1561 caatagttct tttacaatgg tgaaaaagca gcacatccag tcgctgataa tatcagagga
     1621 aagtccggac tctaaagggc ataaggctgg ctaacggcca ggagcagcga tgctacggaa
     1681 agtgcaacag aaaacaaact acctgacttg atcaggaaaa ggtgaaacgg cggggtaaga
     1741 gctcaccgga acagtggcga cacggttcgc aaggcaaacc cccttaagag caaggtctaa
     1801 tagagatgcc atctgccgag gcagataagg gttgcccgcc cgatgcatcc gggttgaccg
     1861 ctagaggcgt caggtaactg ccgtcccaga tgaatgactg gattttacag aatccggctt
     1921 atgtgctgct ttttgactat tgtaagtaat catcttcaat gcccattgct atcgggaaaa
     1981 ttcccgacca gctgagccat accgctgctt caagatcact cttatttatt gtttcaatgg
     2041 tgtcattcac agtatggtaa taagaatggg ttccgagagt tgagagcaat accgccggaa
     2101 ctcctgcata gtagaaacaa acatggtctg atgcatctag cggctgggca ttattgattt
     2161 caagatcgag ttccaattta tctgcataac cgctcattac atctgcaagc catctgtttg
     2221 aagaaagtgt cgccccgtaa actgcgaggc ctgatccgtc acctgctccc accatatcaa
     2281 tactccacgc cgccacagtc tgtgaaatcg ggaaggcagg attttccaca tgttcacaag
     2341 atccgatgag tccaagctct tcagcgttcc atgcggcaaa aacaacagta cgggcagttt
     2401 tatgaccgcc aaggacaaaa gctcttgcaa gttccataac aactgccgta cccgatgcat
     2461 tgtcatctgc accgtaatag atacctccgc tgacaggatc ttttccaaga tggtcaacat
     2521 gcgctccgat aacgatcacc tcttcagaaa gctcctgatc agttcccggg agaacaccta
     2581 tcacattgtc tgctctcacg gtttcaatct cagtctctac ttttatttca gcattaattc
     2641 cagtttcaga gctttgcggc acaaggtcgg catcaattga tcctgaccac gatttcatat
     2701 ccggtatcgt ctcttccatt gtgtcacggt cggcaaatat gactggcaga tcctcaacaa
     2761 agtattctcc gcccagagtc attcccgccg gtactgcacc tgattcctga taatgattca
     2821 cgactattac tccagctgct ccatgaatag cggcattctt tgatttataa ccgaaattcc
     2881 aaagacaggg agctgctacg cacgcatcat tagcaggaca gctgttgtgg actgattcca
     2941 gattgccagg tccgcgacga atgacaagag cgatcttccc tgtcacatca ataccggcga
     3001 aatcatcata tcccgtttca ggaagaggac agtcaggata ttcagacttt ttaaaaggtg
     3061 gtacagtaat cccgtgacct gcaaaaacca cttcagcaga cacatcacct cttccggaat
     3121 attgcataac ctgaaatcct gtttctgttg aaaggagcgt tccatcaatt tcagcttcag
     3181 gagttcctgt gatcacccac tgatcgaaac tgaatgactg aagatatgtt tcaccatcac
     3241 ctgccggcat aagaccaagc tcatcaaaaa gagctctgac atattccaca gaatcagcat
     3301 taccttgagt tccgggctgt ctgcctgtac ggtcagcgtg ggcaaaccat gcaacatcgt
     3361 tatagacccg ctgaatatca aaactgaatt tttctgtcga atcttcatca ttttcactgt
     3421 caaccggatc atcatccgga atatcatcag attcctcata aactgcatca tcaacagatt
     3481 cttcaagatc actcttaacc ctgccgtcat cactgcaacc ggcaaaaaat atcaggatcg
     3541 ttaaggatat taaaaatttc ttcattgcaa acctttagat ttgttactga taaacgacaa
     3601 aagatgtata taccgtcctt tcattataaa ctctcagtcc ggcccttcca ctcatatcct
     3661 gctgaacatc accgcctgaa attacaactt ctccggttcc tcccgcctca tccataaaag
     3721 aacagcgaat ctcagaaccg ttataaaaaa cccttacttt gtgaatttga tcgtctgtgg
     3781 caggcgctgt agctgttgcg cctttgacaa atccgatact tgaaccgccg gcatacctcc
     3841 agatagcaag ctcgttgtta tctctgcgaa aaagacatgt gtaccagtga gtatcattgc
     3901 tgacctgctg cacagaaaac agcaattccg cataactgtt tccgcccaga tctccttcat
     3961 cgtaccggaa agttacttca actgaaaaag gaccattggg aatttgataa gaatagtaat
     4021 tgttctgacc gtacggtgat gttgaaatgc tcatttcact actgccgtaa ttccatgttc
     4081 caccagttgg ggaccatgac ggctgctgat caataaacgg ttcgaaaaca gttatctgag
     4141 aaagaaggtc gtaattgaac ggcgcttcac aaacgtctcc tatcccgtca ttctgtgtat
     4201 ttttctgacc agagttaaaa cttgcaggac agttgtcgca gttgtcatct attccgtcac
     4261 cgtcttcatc aagtccattg cctatgcagc cgctgttacc cgtatcacct gtattgcctg
     4321 tatctccgga atttcctgta tttcctgtat cacctgtatt tcctgtgttt cccgtatctc
     4381 ctgtgtttcc cgtattaccg gtatctcccg aattgcctgt atctcctgtg ttgccgctgt
     4441 cacctgaatc accgctgttt cccgtattgc ctgtgtcgcc tgtgtcgcct gtgtctcctg
     4501 tgttgccggt atttccagaa tttccgctgt cacctgaatt gcctgaatct ccattatctt
     4561 ctttttcgct gcatgtaatt gtgttaaagc cggaacaatc atcgagacac tttgcctttc
     4621 cgccaataaa cttagagctg tcgatatcaa cacagttgac aacattgccg tcgcaaactt
     4681 cacccttgtc aaccatattg tttccgcaga tttgaggagt gtcgctgtct tcatccggct
     4741 ccgttgagca ttcgtcggat aaatcgtcgg ataaatcgtc agataagtca tcggataagt
     4801 catcggataa gtcatcatct gcagccgccg tttcatcatt ctttggaact atctttttga
     4861 catccacatt acaacttatg aaagttattc ctgtaaaaag aaccgaaaat accaccagat
     4921 atctttcaaa caatttcatt ttcccctccg gataaataag aaaactgata aattatatgt
     4981 cttttaagtt attagtcaaa aattgtattt gtaacattct aaaaacatta gctttttctt
     5041 tcttgcattt ttcttgctaa aggattaagt gtattaaagt tatttaggag gcggcaatat
     5101 gaaaaaatca atcgtaattc ttttccttgg attaacattt ttctttgttt cgtgcggtga
     5161 aaacaaaaag gcaaatgacg agtcaggaac tccggatgag gaagtgagtg atgcggaagt
     5221 tgatgaggac gggcttacaa atgacgaaga tgctgcagat gagtctttca gcccttttat
     5281 tgatgcattg aagaaagatc ttgaaaaaag tgatgctttg ggtgtgtcgg ttgcagttat
     5341 gaaagggggc aatgttgttt ttgcacatgc attcggcttt aaggatgccg gaagaaaagt
     5401 tcctctcact ccggaaacat tgatgcagat cggttcaacc actaagcaga tgactgcgac
     5461 ggcgcttctt cagaaggttg aagaagaaaa agtggctctg gaaaatacga ttaacgatgt
     5521 tctgcccgat cttgagttca aacttgatga aacctgggat gattccatta ctgttcatca
     5581 tcttctgagc catcagggtg gattttatga ctggatcccg tgggatttca tgccggaaga
     5641 tgacatgctt gaagggatcg ggagcactta cgcaaaaaaa tattttctga tgaaccctcc
     5701 gggagctttt tataactatt caaatcccaa ctttgccttt gcagggctta tcacggaaac
     5761 gcttgacaac cgtttctggc ctgacataat gcgtgaagat atttttctcc ctcttggcat
     5821 ggaaagaact tatttaagaa aaagtgaagt taaaaaagac ggcgattttt ccgattcgta
     5881 cggttatgac tacaacgaaa ttgaaagtgc agaactgaca gacatcacaa ttgacaagat
     5941 cgctgattcg gcattcaccc gtccggcagg gcttgtctgg acaactccgc ttcaaatgat
     6001 gaaatgggct gattttataa tgaacggcaa caacgaagtt cttggcgata aatatagaaa
     6061 aatgataacg actccccatg ttgacactct ttacggcgca ggaacaatgc attatggtta
     6121 cggaatgatg gttgagaccg gatacatgga taaagctgga aaatggtatg aaattcctgt
     6181 ctgggaacac ggcggaaaca caatatcatt ttctaacatt ctctatattc ttccagatca
     6241 ggattttgca gtttcgatct gttcaagcgg ctatggcact gattttactg aaagtcttga
     6301 tgttgcgata acgactctcg ctgatctgcc tgaaccatct gaaaccgtgc caaaatacga
     6361 aactgaccct gaaaaattcg acgatcatgt cggaacctac aacgatgcat acaatgtcgg
     6421 aacaatgatc ataactcgag atggcgacaa acttcttgtc agcgcaccca cccttgaagg
     6481 atacaactat gaagtgggtc ctgaacttat tcccgtttca agcgacatct tctttctcta
     6541 tcttgacgga caaccgcttg acatcacatt cgtcaaggaa gaaggacact caaaatacat
     6601 gagaaacaga atattcgtcc tcacaaaagt tgaagaagaa gaaacgctca taacaccaac
     6661 atcgcaacag atcgaaaact tcattttaag atcaaaacgc aaattactgc cttgactttt
     6721 gcacaaatta cgacacagac ctgaatcaaa acattgccat aactaatttg acaatcatct
     6781 gaaacttgat aagaataaat acgaattgct gaaagtgggg agtgatggaa aaacacgaaa
     6841 tagaatctgc cagagggcag tttctggttt atcaggctga ggatgggaaa ttaaaacttg
     6901 atgtacgctt tgaggatgaa tcggtgtggc tgactcagca gttgatggca gatttgttcc
     6961 agacttctaa gcagaatgta agtcaccata ttaattcgat ttatgaagaa agagaactcc
     7021 tgcctgaggc aactgtcaaa aaatatttga cagttcgatc agagggaaat cgtgaagtaa
     7081 aacggctact tgattattac aatctggata tgattatttc agtgggttat cgtgtaaaaa
     7141 gtcatgttgc aacccgtttt cgtatatggg caacccaaaa acttactgaa tttattaaaa
     7201 aaggattcgt gctggatgat gaacggctca aaaaccccga tcttcctttt gactactttg
     7261 aagaactcga aaaacggatt caggatatac gaacatccga acgccgtttt tatcagaaaa
     7321 tcacagatat ttatgccaca agtattgatt atgatccgac tcttgatata agcctcgaat
     7381 tttttaaaac agttcagaac aagatgcact gggctattac cggacagacg gcagcagaaa
     7441 taatccattc ccgtgcggat gcaaataaac ccaaaatggg actgactaac tatcgtggat
     7501 caaaagtaag aaaacaggat gtgacaatcg caaaaaatta tatgaatgaa aaagaacttg
     7561 ccgaactgaa taaccttgtg gaacaatatt taatttttgc acaaggtcag gcaatgcggc
     7621 gtattccgat gtatatgagt gactggatta aaaaactgga tgcttttctg aatataaatg
     7681 acaggaaaat tctggatcat gcaggtaaaa tttctcatga attagcaaaa caactagctg
     7741 aaagtgaata tgaaaaatat catgtaaaac agttgcaaac tttcagcaaa gaactgagcg
     7801 actttgacaa agcagtgaaa caaattgaat cttccaaaaa agaaataaag aaataagctg
     7861 aaaaaccttg aaaatatttt aagggaagcg agggagaatg tttatttcaa tgctgacaat
     7921 taagatcaaa aaaatcaagg attttgtgaa gtgaatacac ccaaaagaca tcattatcta
     7981 cctgagttat atttaaaaag attctgtaca aatgaacagc tttttattta cgataggaaa
     8041 aagaatgaaa ttaggccgca aaccccagta aacaccggag ttcaatttca ctattattcg
     8101 tcaatcaatg aaaaaggaga aaaaaaacct gaaatcgaaa ctgatcttct ttctagaatt
     8161 gataatgacg caaacaatgt tatagaaaaa atactgaaaa atgagcaaat aaatgagttt
     8221 gataaagaaa aactttcaat ctttttagga tttatggtgc atagagttcc ttattttgaa
     8281 aactccttta attctatgta ttcctacatt aacaaaaaac ttttgaactc aatattttgt
     8341 agtgaagaat tagtacaaat aatgactgcg gataacgagg aaaatataac cgctgaagag
     8401 tttgtggatt tttataaaaa ttccaatctt aaaatcaccc cgcatagaaa cgaaagcctc
     8461 agatttatgt tgagtcagag tctagttgcg tcagaatgct tcatgaaata caaatgggaa
     8521 atattatact gttcttcaga atcgtctttt ataacatcag acaatcctgt gataactaca
     8581 aaatttgttg aacaatcaac cggtgaaaat cttgggaatt tgatactttt ccctttatct
     8641 aacaacttat gtctaagtat gtttcataaa ggaacagaag ttgtttttaa tgaaattaat
     8701 cagtcctcag tagagaaaat taatgccgta attgcaacaa attcctacca gtatattttg
     8761 gggaaagaca aaactataat tgaaaaaatc gttgcagaaa acaagatcca aggaataaat
     8821 aacaagaaaa tgta
//
LOCUS       DAOOYB010000111         8753 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_8873_length_8753_cov_8.275236, whole genome shotgun sequence.
ACCESSION   DAOOYB010000111 DAOOYB010000000
VERSION     DAOOYB010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8753
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8873_length_8753_cov_8.275236"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(167..1627)
                     /locus_tag="PL056_07745"
     CDS             complement(167..1627)
                     /locus_tag="PL056_07745"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HPM46946.1"
                     /translation="MKKMIFLLILFGFFSLLADTLEEKMLSDAYTIKKNIKEFIDLNN
                     EELFDDAEKDENSLKKLDEFVNKKITVYEKSLKETIVIYIKKKGYAAFEKFLKGKTMD
                     IADIIIGVENGTFNLMNYTKGTVYYNYKSLSLLSNLIGEQSKENTEKGKKDSWDIIRK
                     SKHIGNDITEHTFFRNNEPIAQHIFKDGIIYKIKGEKIEGIVGCYNEDGEFVSKTEYK
                     SNIKNGKETSFYDSGNILMVSEWIDGKANGERRDFYENGQIKNIFNYREGIATGAFKT
                     YYPTGVLSLEGSLKNDKFDGVFKYYSKNGNLSFEEVYENGKNVGTSKSYTTTGKISML
                     KEYNKKGGYIARYYFLDEVKYIERSDGEEPVVLDKETGLIWQKNHKDNSMRNPLSFCK
                     DLDYGGYSDWRLPDIKELSTLINTSREERASIFPGILAENYLSSSKLDKPCDGGFFSK
                     VKIVNFYDGEIDETCRLKNEKINYLCVRSSKKEPDN"
     gene            complement(1650..2156)
                     /locus_tag="PL056_07750"
     CDS             complement(1650..2156)
                     /locus_tag="PL056_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46947.1"
                     /translation="MIKTRRTDSVMCDFSIENLKIPIFSFSEIFRFREKLTNLAGIYS
                     FLWNGDKNLIKDANLKFVLKGSKNKEHESKYWTYGDPVFLYIGKSTNFYKRFLLHRRP
                     SAKSGHQLFKGIKELFPEECNEKLIEIINKNISVSYILIEDFVERFYAENKAIGDLRP
                     WFNVDCER"
     gene            complement(2242..3165)
                     /locus_tag="PL056_07755"
     CDS             complement(2242..3165)
                     /locus_tag="PL056_07755"
                     /inference="COORDINATES: protein motif:HMM:NF018009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HPM46948.1"
                     /translation="MPNSFVRGYSKSNIEYMRKFFLLYSGRLEEKTQTPSGQLEKKKT
                     QKESGESEKTEIMLSDKITGQIVVKHLPLSWSHYVFLIGVKNEKERTFYEIEAANANW
                     SLKELKRQFNSSLFERLALSKDKAGIRKMAEKGVVIEKPQDVIKNPFVLEFLGLDENT
                     RYTETDIETAIIDKLETFLLELGKGFLFEARQKRFTFDEDHFFVDLVFYNRILRCYVL
                     IDLKIGELKHQDLGQMMMYVNYFDRFVKLEHETPTIGIILCKKNKSSLVEITLPENSN
                     IRSAEYQLYLPSKEELKKQIEEAEKEFSEKE"
     gene            complement(3169..3465)
                     /locus_tag="PL056_07760"
     CDS             complement(3169..3465)
                     /locus_tag="PL056_07760"
                     /inference="COORDINATES: protein motif:HMM:NF036995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 N-terminal domain-containing protein"
                     /protein_id="HPM46949.1"
                     /translation="MTDRNSYNDLVSEIKAIISTARAQSVRSINSMQVLSNFMIGRRI
                     IQFEQGGKERAEYGKETLRNLSDELTKEFGRGYSKSNIEYMRKFFLLYSILLFE"
     gene            3661..5256
                     /locus_tag="PL056_07765"
     CDS             3661..5256
                     /locus_tag="PL056_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013929412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HPM46950.1"
                     /translation="MSRLHEEIAKRKTFAIISHPDAGKTTLTEKLLLFGGQLVTAGAV
                     KSNKIQKSTTSDFMEIERQRGISVATSVMSFEYRDLKINILDTPGHKDFAEDTYRTLT
                     AVDSVILVVDSVKGVESQTERLMNVCRMRNTPVIVFINKLDREGQSPFELLDEIEAKL
                     SIKVRPLTWPISRGKTFKGVYNLHKKNLNLFAADSQRIAKNIIEIKDISQPELNDHIG
                     DFADKLREDVTLIEGVYDPFDKEDYLLGNIAPVFFGSAVNSFGVNELLDTFCDIAPNP
                     MFRMTDKRKISANEDKFTGFVFKIHANLDPKHRNRMVFVRICSGIFEKNKYYHHVRLD
                     KKLKFNTPASFVGQKKETIDEAFPGDVVGLYDVGNFKIGDTLTEGEELNFQGIPSFSP
                     EIFKLIQNRTFEKVKQLDKGVLQLSEEGVAQLFIQDQGRRKIIGTVGELQFEVLQYRL
                     LNEYGAACSFTTLNYTKARWITSKDPKKIDEFCKFKSNQIAKDKDGNLVFFAESDWML
                     QYYMKDHPDIEFHTTSEFKTRID"
     gene            complement(5259..6026)
                     /locus_tag="PL056_07770"
     CDS             complement(5259..6026)
                     /locus_tag="PL056_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46951.1"
                     /translation="MKKRISFVFAVTFFITFSAYANEKWSVQKNAGALSACYQSVSTM
                     YCAGSMNKKVTETDLKHYLSVYLYFSKDDAKKSDLSVQPGIMRLIKGSRPEQGDRVLK
                     GAYDYLLREGKSKAIMLSFGAGKNDQKVMSESEQIIEIESVNWPKYIDSSIDSISCTS
                     DQKVNLTFKVTNSLKKDFIIKGEEIELRIMSGRHEILSVGSVTEGSSTIKAGETKSFQ
                     VDIPVTGDMIPSMRYRIIPFIRNIQVSKEDASVLCEK"
     gene            6156..6572
                     /locus_tag="PL056_07775"
     CDS             6156..6572
                     /locus_tag="PL056_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymyristoyl-ACP dehydratase"
                     /protein_id="HPM46952.1"
                     /translation="MRSSELIPQREPFVLVDEIIECSGEKTTTRFSFNDTHILCSDGL
                     LLEAGIIENFAQSAAAGAGHHFVSQGLDIPIGFIGSVDKLKINFRPSAGDLIETEITV
                     AHKVMNVTVVDCVSKVKDKIVATCKMKIFIVDQEQQ"
     gene            6572..6997
                     /locus_tag="PL056_07780"
     CDS             6572..6997
                     /locus_tag="PL056_07780"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008666653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HPM46953.1"
                     /translation="MNELRISTPIIARFSEVDMMAVAWHGSYVKYLEDGREDFGKHFG
                     LEYMTVYDAGFMTPIVRMNIEYKSPLFYGKTAEIETVFINSPAAKIRFEYTVKCSETG
                     RIIALAWTEQVFINAETRELELTPPEFFIDWKKKNGLQI"
     gene            6994..8046
                     /locus_tag="PL056_07785"
     CDS             6994..8046
                     /locus_tag="PL056_07785"
                     /inference="COORDINATES: protein motif:HMM:NF014819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HPM46954.1"
                     /translation="MKKAFIIADNIFSPLGKTSCEVFENMAHGKSSVKLVENGEICRV
                     PAYLSVFDEKFENKFESICINSIHGAVSESGIDPKDSQTLFILSTTKGNIGVPLNKTA
                     EIIAEKFGFINNPFVISNACISGVVAITIAERLIKKGAYRNIVVTGADILTDFIVSGF
                     QSLKAIGSTVCRPFDVARNGINLGECAATVILSSDAKSGVEVTGGAFNGDANHISGPS
                     RTAEGLYLSISRTMNGFNGLPSFISAHGTATSFNDEMESIGFDRAGLNDVPLNSLKPY
                     FGHTLGAAGIAEIIISAHSIQKGIALKTPGYENHGVTRNITVLKENMRAELTSCLKTA
                     SGFGGCNASVLLVKNK"
     gene            8043..8588
                     /locus_tag="PL056_07790"
     CDS             8043..8588
                     /locus_tag="PL056_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HPM46955.1"
                     /translation="MRLKIIRSCRISNDTVEVDGKTVLKTTETPGTSKQFKEIYLSLN
                     IDYPKFYKMDMLSKLAFLAVEFIKNEGFSEKDTAIIMTNANSTLDVDLKYTKSIDPDN
                     YFPNPAQFVYTLPNVMIGEISIRHNLKGESVFVSTENKDLELLENIAKILEFKNCIAG
                     FIEYENENNFQADIIFCEKNY"
     gene            8602..>8753
                     /locus_tag="PL056_07795"
     CDS             8602..>8753
                     /locus_tag="PL056_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009236900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HPM46956.1"
                     /translation="MEQLIKTLKDQIINELNLEDMTADEIDAQAPLFGEGLGLDSIDA
                     LSLIVLL"
BASE COUNT         2714 a         1738 c         1541 g         2760 t
ORIGIN      
        1 acagaaccgt ttgcaagcag tttttttatc ctcgtccttg gcgaatcggg ataaagcttt
       61 atcagcaagt ccataagctt aatgttttca gtcactttat gtttcaagag aattccatta
      121 aaaagtttta atccttttgt attccaacct ctataaatga ggcaaactaa ttatcaggtt
      181 ctttcttgct tgatcgaaca cataaataat ttattttttc gttttttaag cgacaagttt
      241 cgtctatttc accatcatag aaattaacga tcttaacttt agaaaaaaaa cctccatcgc
      301 aaggtttgtc taatttagat gacgaaaggt agttttcggc taaaatccca ggaaatatgg
      361 aagcacgctc ttctctcgaa gtatttatta atgttgataa ttccttaata tcaggcaaac
      421 gccaatctga ataaccgccg taatccaaat ccttacaaaa agataatgga ttacgcattg
      481 aattatcttt atggttcttt tgccaaataa gacctgtttc cttgtctaaa acaacgggct
      541 cttcgccatc tgatctctct atatatttta cttcgtccag aaaataatat cttgcgatat
      601 acccaccctt tttattatac tcctttaaca tggatatctt tcctgttgtt gtatatgatt
      661 ttgaagtccc cacattttta ccgttttcat aaacttcttc aaaagataga ttgccatttt
      721 tagaatagta tttaaaaaca ccatcaaatt tatcgttttt taaagatcct tctaacgaca
      781 aaacacccgt aggataataa gtcttaaaag ctcctgtcgc tatcccttct ctgtaattaa
      841 agatattctt aatctgacca ttctcataaa aatctcgtct ttcaccattt gctttaccat
      901 caatccattc tgaaaccatt agaatattac cactatcata gaaactcgtt tctttaccgt
      961 tttttatatt agatttatat tccgtcttcg atacaaattc accatcttca ttgtaacatc
     1021 ccacaattcc ttctatcttt tcccccttta ttttataaat aattccgtct ttaaaaatgt
     1081 gttgtgcaat tggctcatta tttctgaaga aagtgtgttc ggttatgtca ttgccaatgt
     1141 gtttcgactt tcttataata tcccatgaat ctttcttgcc cttttctgtg ttttcttttg
     1201 attgctctcc aataagattt gacaacaaag acaaagactt gtaattgtaa taaacagttc
     1261 cttttgtgta attcattaaa ttaaatgtcc cattttcaac accgattata atatcagcta
     1321 tgtccattgt tttgcctttt agaaactttt caaatgccgc atatcctttt ttctttatgt
     1381 aaataactat tgtttctttt aatgattttt cataaacagt gattttttta tttacaaatt
     1441 catcaagttt tttcaagcta ttttcatctt tttcagcatc atcaaaaagc tcctcattgt
     1501 ttagatcaat aaactccttg atattttttt ttattgtata ggcatcggaa agcattttct
     1561 cttctaaagt atctgccaaa agtgaaaaga aaccaaataa tatcaaaaga aaaatcatct
     1621 ttttcatggc agttaactcc tttttatggt tatctttcac aatcgacatt aaaccacgga
     1681 cgaagatcac ctatcgcctt gttttctgca taaaatcttt caacaaaatc ctctatcaga
     1741 atgtacgata cagaaatatt tttgtttatt atttcaatta atttttcatt acattcttcg
     1801 ggaaacaatt ctttgatgcc tttaaataac tgatgtcctg attttgcgga tgggcgtctg
     1861 tgaagtaaga agcgtttata aaagtttgtc gatttaccaa tgtataaaaa tactggatca
     1921 ccataagtcc aatatttgct ttcatgttct ttgtttttcg atccttttag aacaaatttc
     1981 aaatttgcat cttttatgag atttttatct ccattccaca aaaaagaata tatcccagcc
     2041 aaattagtta atttttctct gaatctaaat atttcactga aagaaaatat aggaattttt
     2101 aaattttcaa tactgaaatc gcacatcaca ctatctgtcc tccttgtttt gatcatctat
     2161 aatccccctc aaattcataa tataatagac gaaaggtcta aaactgcaaa attttctgct
     2221 ttttgcaccc gtgtaattgt tttattcttt ttctgaaaat tccttttctg cttcctcaat
     2281 ctgttttttc agttcttctt tgcttggaag atataactga tattcagcgg aacgaatatt
     2341 tgaattttcc ggtagagtta tttcaacaag agatgactta tttttcttgc agagaataat
     2401 tccaatcgta ggtgtttcgt gctcaagctt cacaaaacgg tcaaaataat tcacatacat
     2461 catcatttgt ccaagatcct gatgtttcag ttcgcctatt tttaagtcaa tcagcacata
     2521 acatcttaaa attctgttgt agaaaacaag gtcaacaaaa aagtgatctt catcaaaagt
     2581 aaacctcttc tgacgggctt caaaaagaaa acctttgcca agttccagca gaaaggtttc
     2641 aagtttatca ataattgctg tttcaatatc tgtttccgta tagcgggtat tttcatcaag
     2701 acccagaaat tcaaggacga aagggttctt tataacatct tgcggttttt cgatcacaac
     2761 acctttttct gccatttttc taatgcctgc cttatcttta cttaaagcga gtctttcaaa
     2821 aagagacgaa ttgaactggc gcttcaattc tttcagtgac cagtttgcat ttgctgcctc
     2881 aatttcataa aaagtccgct ctttttcatt ttttacccca atcagaaaaa catagtgaga
     2941 ccagcttaaa ggaagatgtt taacaacaat ctgtcctgta attttatcag aaagcattat
     3001 ctctgttttt tcagattccc cagactcctt ctgggttttc tttttttcca attgcccaga
     3061 cggtgtctgg gttttttctt caagcctgcc ggaataaaga agaaagaact ttcgcatata
     3121 ttcaatattg ctctttgaat aacctctgac aaaagagttc ggcagaggtt attcaaagag
     3181 caatattgaa taaagaagaa agaactttcg catatattca atattgctct ttgaataacc
     3241 tctgccgaac tcttttgtca gttcatccga taagttcctg agagtttctt tcccgtattc
     3301 ggctctttct tttccaccct gctcaaactg tatgattctt cttcctatca taaagtttga
     3361 taatacctgc atggagttaa tgcttcttac actttgagcc cttgcagttg agataatggc
     3421 ctttatttct gaaactaaat cattgtatga atttctgtct gtcatttaaa acctccaaac
     3481 ataaaacagg gttgatacat ggatcaaccc ctacatcaca aatcaaattc atgttattct
     3541 aaactttaga aatgatttat caaattattt ggttgtattg acataagggg ggtgagttat
     3601 gttgacagtt taagtatttt atagtacttg agcacagttt tctgtttttt tagaggtaat
     3661 atgtcaagac ttcatgaaga gatcgcgaaa agaaagactt ttgcgataat aagccatccg
     3721 gatgcgggaa aaacgactct cactgaaaaa ctgctccttt tcgggggtca gcttgttaca
     3781 gcgggagctg tcaaatcaaa taaaattcag aaaagcacta cttcagattt catggagata
     3841 gagaggcaga gagggatttc agttgcaact tcggtcatga gctttgaata ccgcgacctt
     3901 aaaatcaaca ttctcgacac tcccgggcac aaggattttg cggaagatac atacagaact
     3961 ctcactgccg ttgacagcgt aattcttgtc gttgattcgg ttaaaggtgt agaatcacag
     4021 actgaacggc tgatgaatgt gtgccggatg agaaacactc cagtcatcgt tttcattaat
     4081 aagctcgacc gtgaaggaca gtcccctttt gaacttcttg acgaaattga agcgaaactc
     4141 agcataaaag tgcgtcccct cacctggccg atcagcaggg gaaaaacatt caaaggggtc
     4201 tataatctcc acaaaaagaa cctgaacctt tttgcggcag attctcagcg aattgcaaaa
     4261 aacatcattg agatcaagga catatctcaa ccagaactca atgaccacat cggtgacttt
     4321 gcagataagc tcagggagga tgtgacactt attgaaggcg tgtatgaccc gtttgacaaa
     4381 gaggattacc ttttgggcaa tatcgctcct gtgtttttcg gaagcgctgt caacagtttc
     4441 ggagtgaacg aacttcttga cactttctgt gatattgctc caaacccgat gttcagaatg
     4501 accgacaaga gaaaaatttc agcaaacgaa gataagttca ccggatttgt gtttaagatc
     4561 catgccaacc ttgacccgaa acaccgaaac agaatggtat ttgtccgtat ctgttcagga
     4621 atctttgaaa aaaacaaata ttatcaccat gtgcgactcg acaaaaaact taagttcaac
     4681 actcctgcaa gctttgtcgg acagaaaaag gaaacaattg atgaggcatt tccaggcgat
     4741 gttgtaggac tatatgatgt cgggaatttt aaaataggag acacgctgac tgaaggagaa
     4801 gaactcaatt tccagggaat cccttctttc agccctgaaa tattcaaact tatccagaac
     4861 agaacatttg agaaagtaaa acagcttgat aaaggggttc tgcaactttc tgaggaaggt
     4921 gtcgctcagc tttttattca ggatcaggga agaagaaaga taatcggaac agtcggggaa
     4981 cttcagtttg aggtactcca gtaccgcctc cttaatgaat acggtgcagc atgctcgttt
     5041 acgacgctta attacacaaa agcccgctgg ataacatcaa aagaccctaa aaagattgat
     5101 gaattctgca aattcaaaag caatcagatc gccaaggaca aagacggcaa ccttgtcttt
     5161 tttgccgaat cggactggat gcttcaatat tacatgaaag atcatcccga catagaattc
     5221 cacacaactt ctgaatttaa aacaagaatc gattaatatc acttctcaca aagaacggat
     5281 gcatcctctt ttgaaacctg aatgtttctg atgaaaggga ttatccggta tctcattgac
     5341 ggtatcatgt caccagttac gggaatatcg acttgaaatg actttgtttc acccgctttt
     5401 atcgttgaac tcccttcagt taccgacccg acagaaagta tttcgtgtct tccgctcatt
     5461 attctcagct cgatctcttc acctttgata atgaaatcct ttttcaagct gttcgtgacc
     5521 ttgaaagtaa ggttcacctt ctgatcactt gtgcatgata ttgaatctat tgatgaatct
     5581 atgtatttag gccagtttac agattcaatt tcgattatct gttcggattc ggacatgact
     5641 ttctgatcat ttttgccggc tccgaaagag agcattatcg ccttactctt cccctctctt
     5701 aaaagataat cataggcacc tttaagaacc cggtcaccct gctccggtct tgaacctttg
     5761 ataagccgca ttattccggg ctgtactgat agatcgctct ttttcgcatc atcttttgaa
     5821 aaataaagat agaccgaaag atagtgctta agatcggttt ctgtaacttt cttattcatt
     5881 gagccggcac agtacatcgt cgaaactgac tgataacagg cacttaaagc ccccgcattc
     5941 ttctgaaccg accatttttc atttgcatat gcggaaaatg taataaaaaa tgtaacggca
     6001 aaaacaaagg aaatcctttt tttcatcaga ccctccttga taacagagta cataaaatta
     6061 ttgcaattta tgaagttaag tcaatgaaag ttgcgaaagt tgcttaacta tattatattg
     6121 tcgtgttttt ttttgaactg aaagtgagat ttatcttgag atcatccgaa ctgataccgc
     6181 agagagagcc gtttgttctt gtcgatgaaa ttatcgaatg ttcgggcgaa aagaccacaa
     6241 cccgtttttc atttaacgac actcacattt tatgcagcga cggacttctg ctcgaagcgg
     6301 gaataattga aaattttgcc cagagcgcag cagcaggtgc aggacatcat tttgtgtcgc
     6361 agggacttga cattcctatc ggtttcatcg gatctgttga taaactgaag ataaatttca
     6421 gaccttcagc gggagatctc attgaaactg agatcacagt cgcccacaaa gtgatgaatg
     6481 tgacagtcgt tgactgcgtt tcaaaggtta aagataaaat tgtcgcaaca tgcaaaatga
     6541 aaatattcat tgtagatcag gaacaacaat aatgaatgaa ttaaggattt caactccgat
     6601 tatagcgcgg ttttccgaag ttgacatgat ggctgtcgcg tggcatggaa gctatgtgaa
     6661 atatcttgaa gacggcagag aggacttcgg gaaacatttc ggacttgaat atatgacagt
     6721 ttacgatgca gggttcatga ccccgatcgt gagaatgaac attgagtata aatcacctct
     6781 cttttacggt aagaccgcgg aaatagaaac agttttcatc aattcccctg cggcaaagat
     6841 aaggttcgaa tacacagtaa aatgctcaga aactggcagg atcatagctc tcgcgtggac
     6901 agaacaggtc ttcatcaatg ctgaaacgag ggaacttgag ctgactcctc cggaattttt
     6961 cattgactgg aaaaaaaaga acggacttca aatatgaaaa aagcattcat cattgcagac
     7021 aacatatttt cccctcttgg aaaaacaagt tgcgaagttt ttgagaacat ggctcatgga
     7081 aaaagcagtg taaaacttgt tgaaaatggt gaaatttgca gagttcctgc atatttatct
     7141 gtttttgatg agaaatttga gaacaagttt gaatcgatct gcataaactc gattcatggt
     7201 gccgtttcag aaagcggcat agatccaaaa gattctcaaa ccctctttat tctctcaact
     7261 actaaaggaa atatcggtgt tcctcttaat aaaaccgcag aaatcatcgc tgaaaagttc
     7321 ggattcatta acaatccctt tgttatttcc aatgcctgca tttctggtgt cgttgcgata
     7381 actattgccg aaagattaat aaaaaaggga gcttacagaa acatcgtagt tacgggcgct
     7441 gatattctga cagattttat tgtaagcgga ttccagtcac tcaaagcgat aggaagcaca
     7501 gtgtgcaggc cttttgatgt ggccaggaat ggaataaatc tcggtgaatg tgcggcaaca
     7561 gttatattaa gttcagatgc aaagagcggt gtcgaagtga ctggaggagc tttcaacggg
     7621 gatgccaacc acatttcagg accgtcaagg accgctgaag ggctctatct ttcaatttca
     7681 agaacgatga acggtttcaa cggacttccc tctttcatat cggctcacgg gactgcaacc
     7741 tcattcaatg atgaaatgga atctatcggg tttgaccggg caggcctcaa tgatgtcccg
     7801 ctgaacagtc tcaagcccta tttcggacac actctcggag cggccggcat tgctgagata
     7861 atcatttcgg cacattcaat acagaaaggg atcgcactga aaactcccgg ttacgaaaat
     7921 cacggtgtaa caagaaatat cactgtttta aaggaaaata tgcgggcgga actaacaagc
     7981 tgcttaaaga cagcttccgg ctttggcgga tgtaacgctt cagttttatt ggtgaaaaac
     8041 aaatgagatt gaaaataata agatcttgca gaatttctaa tgacactgtt gaagttgacg
     8101 gaaaaactgt tctgaaaact actgaaacac caggaacttc aaagcagttc aaggagatat
     8161 acctttcttt gaatattgat tatcctaaat tctacaagat ggatatgctt tcaaaactcg
     8221 ctttccttgc ggtggaattc ataaaaaacg agggtttttc tgaaaaagat accgcaatta
     8281 tcatgacaaa tgccaattca acgctcgatg ttgacttgaa atacacaaaa agcattgacc
     8341 ccgataatta ctttccaaat cctgcccaat ttgtctatac actgcccaat gtcatgatcg
     8401 gtgaaatttc gataaggcac aatctgaaag gagaatctgt ctttgtgtca acagagaaca
     8461 aagatcttga actccttgaa aatattgcca aaatactgga atttaaaaac tgtattgccg
     8521 gtttcattga atacgaaaat gagaacaatt ttcaagctga tattattttt tgtgaaaaaa
     8581 actattaaaa cggaggagaa aatggaacag cttattaaaa cacttaaaga tcagatcatc
     8641 aacgaactca accttgaaga catgaccgca gacgaaatag atgcacaagc tccccttttc
     8701 ggtgaagggc ttggtcttga ttcaatagac gcattgtcac tcattgtcct tct
//
LOCUS       DAOOYB010000112         8645 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_9019_length_8645_cov_14.430501, whole genome shotgun sequence.
ACCESSION   DAOOYB010000112 DAOOYB010000000
VERSION     DAOOYB010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8645
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9019_length_8645_cov_14.430501"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1686)
                     /locus_tag="PL056_07800"
     CDS             complement(<1..1686)
                     /locus_tag="PL056_07800"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HPM46957.1"
                     /translation="MQIERFAVSLLTLCFYNIYAAENSVVVTGSRNGQIDSVSASSTV
                     TAKEIEEKQSVTAGDAVREMPGVYVSGDGRTGQAQFVFVRGFRSSDVLILLDGVELRN
                     PLSPDGATDLSLSPENIAKIELLKGPQPVLYGSGALAGVLNIQTKKGFGKPSFSTSFS
                     TAVLDMSNERYVPDTFNANISSMGGNCKAYYNGGASFFYTEGISMADSYSGVKEGLYD
                     KTPENDKVIKGSGYFRTGVTIDNDNEWDLIFRVAKGKNEIDDGPGIGQDDPDRYLDSE
                     DFLMKSGLVSSFFDKKWTMTTDFSIFYGTLFDSDSAEHGKVTGDLDSSYSAVSGVLNW
                     ENAIIPANWYELTLGVELKKEWGEADYSDYSAGRKLDLSFTPSTDLSTGIYAFNVFKP
                     VKGLEINGGIRAQANFYQIALLDEKTDNPLPAEKRKSIEPLFSAGISYEAPSQTVFKA
                     RIARGTKTPTLFQRFSRYADLYNELKPETALGFDGIVQQYFYKRKILVEAGYFYELKS
                     DHISLDKSGKYSNRYMVENHGLEFSLQTKQIYGLSLKAAYTWLFGLKEYKVVEY"
     regulatory      complement(1705..1819)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(1855..2109)
                     /locus_tag="PL056_07805"
     CDS             complement(1855..2109)
                     /locus_tag="PL056_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002764006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HPM46958.1"
                     /translation="MSIVSLIGIAAAICTTVSFVPQVLKTIKTGQTKDISLSMYLIFT
                     TGIVLWLIYGIMIRDLPIILANTVTVVLTSIVVVLKIRNG"
     gene            2263..2769
                     /locus_tag="PL056_07810"
     CDS             2263..2769
                     /locus_tag="PL056_07810"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPM46959.1"
                     /translation="MKDSKGFSVIELMIVISILNILGTIAIAGYTDYTKKARVAEAPL
                     MLKSLVIRIDEYMKDAPPKSQGKCELVDSNGRCQDNFPKNAEDTGFRTNRDSVFGNFF
                     EFKVKTPHPTGCGEKFYNRYAYALPLVNNDIPEDYQFMCMNERFDFFHSSKSSDPGKP
                     AGKGSGKK"
     gene            complement(2779..5088)
                     /locus_tag="PL056_07815"
     CDS             complement(2779..5088)
                     /locus_tag="PL056_07815"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HPM46960.1"
                     /translation="MGKFLLQDTTVKKLIRKTLKENQEGYLFFENGDEWKKLFFRNGI
                     LTGAASSSKADYLGQYLISYGVIDTDQFEESYKAGYDPKKSMDDVMKAATPEILKQLI
                     YEKILDTIFIASRWSECLYSVVSEKSKDQKVDVEISVEEISTGLKERVAEFRAVLSVI
                     PTLGSRPKIEHDEIKDHKITDQHEIILNYLGAGKTIDEILSIMTPHNYLLFKCFYQLC
                     SMGILVKGTGAPLSREKVIQLVNESGKSKKSVQHVAIDEQISDKSLSIEFQIDADEDK
                     YRKEVTIYQKLHSEDPDNSIYAHCFGKAESCFIVEFYTKKLSPFAVIGVAKDVDRTGG
                     KKIDAEICEAIKKEGGKLPVRNLIKLLDHRHEIDILTSIEKLISSGAIIEIEPETFID
                     AIKLGRSEFFEKLFKKNEINKMFSTDISANLTPSMLSVISGTTEDDLAEKPGARSFKT
                     GLKPVLYDYEMTYLMLASMIGNYEAVEFLLSNHAKSDLHNGNGVTALMLALQNGYDET
                     ALLLMNKGADVNAKNQNNYSALMIAASKGMAHIVDFMIKLKVDVNQLNSKGQSALISA
                     LRFNHKDIVLSLIAAGTDLNHKDLNGTLPVDHAESPEMAELIRKGTKNAKQIKEKLNK
                     KKDDLEISEKDLIKKERQERSGFLAVILFAMLMVITSAVNVYLLFFPEERYGFSKESK
                     EVVDQLGMEYCNRFKICRTTVPEDVLAKCHGIGISIMSEFFKNVRSCDMELVENCKSC
                     LKAVNCDDFYNIDGSTLSEYCHQCTNACK"
     gene            complement(5193..5708)
                     /locus_tag="PL056_07820"
     CDS             complement(5193..5708)
                     /locus_tag="PL056_07820"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HPM46961.1"
                     /translation="MIFLISFLLLSQIHIPTANMINQKYSAIIEKSVAKYPYMNKSGV
                     KVTMHPGLVKAIIYQESRGNPEAKSHAGAYGLTQIMPQTAKLLKCEYSKLKDPELAIN
                     CSVKFLAALLTYNKGDLIKSLSGYNGGTHSTETKATTTDGFLAGRIYDHPETKQYVVN
                     VLKWFEWFKKK"
     gene            complement(5705..6586)
                     /locus_tag="PL056_07825"
     CDS             complement(5705..6586)
                     /locus_tag="PL056_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46962.1"
                     /translation="MKKQLLAIIAGLFLSLDLSAQLMVASPGTDAAIETQTLLMSNLA
                     IQQRIKEYQHYVEYISQFVEVIASIKNTTSTIRDISKMGRELKERSADEWLADVEKHI
                     NEVMPEYSELKNEMAGMVKGGHEMIKGKYSDYVSKWSGEVKNYHESLLKNYDNHVMFP
                     ELYPAAQKMKDGFTKGESAKKVIHKAWIESGLEYEMKNDIVRKNTFKNYYDEYLKQAK
                     SNDNIEALGLANLMQAQYISVELLENLKKNSDLAIMKEQFLKDMSDAYLEVKKKLESE
                     EKKRNKPSEIFGLKEKK"
     gene            6724..7251
                     /locus_tag="PL056_07830"
     CDS             6724..7251
                     /locus_tag="PL056_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46963.1"
                     /translation="MKNLSVFFIVLITMFLASCTDTSLKPEEAERLIKERLSKMPGRS
                     VSFYLGAMTDPAYLPIYKKIATGKYLTLKEDVFVEAAGKKMPMFEATPEGEKIFKCDN
                     NRCSVEVCKTEFDKFVSIVNKGKFANSTYQVKTICDGEIYNIFRPLADRQYIKPETVE
                     EKAEFEYSKDTWIIK"
     gene            7341..8549
                     /locus_tag="PL056_07835"
     CDS             7341..8549
                     /locus_tag="PL056_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HPM46964.1"
                     /translation="MYHDLEKSFSKMTKRAKKSAIRELLKLTNKPGLISFAGGLPAPC
                     TFPSEELKEITNEVLTKEANIALQYGTTEGDDLLREQLTKRYQSMGLNITKENLVITT
                     SSQQALDFLGRVFINEGDKIIVELPSYLGGLQAFNAFGADMEGIKSDEFGMCPVALEK
                     KMAELSSLGKKPKFIYVIPDFQNPSGITMPESRRKEIIDIAHKYEVLIVEDSPYRELR
                     FDGEPQKTIYQLDEMGTTITLGTFSKIFVPGFRIGWIIAHPAIVDKFVTTKQSVDLCT
                     PAFVQRIAARYMEKGYFEKNLKSTIKLYGRKKEVMMESFEKYMPEGVTWTNPEGGLFL
                     FVYLPEHMDAQKLFLTAVENNVAFVIGQVFHCDGSGINTMRINFSFCDDEKIVEGVKR
                     LAAAIKEYKN"
BASE COUNT         2431 a         1824 c         1640 g         2750 t
ORIGIN      
        1 atattcaaca actttatatt ctttcagtcc gaataaccag gtatatgccg ctttcaagct
       61 caatccgtag atctgttttg tctgaagaga aaattcaagt ccgtgatttt caaccatgta
      121 cctgtttgaa tatttccctg atttatcaag agaaatgtga tctgatttca gttcatagaa
      181 atagcccgct tcgacaagga tctttctttt ataaaaatac tgctggacaa tgccgtcaaa
      241 accgagtgct gtttcgggct tgagttcgtt ataaagatcg gcatatctgc tgaaccgctg
      301 aaaaagtgtc ggagttttcg ttcctcttgc aattcgtgcc ttgaaaacgg tctgactcgg
      361 tgcttcgtaa gatattcctg ctgaaaacag cggttcaatg ctttttcttt tttccgctgg
      421 aagcgggttg tctgtttttt catcaagaag tgcgatctga taaaaatttg cctgtgccct
      481 tatcccgccg ttgatctcaa gtcctttgac cggcttaaaa acattgaaag catagattcc
      541 ggttgagaga tccgttgaag gggtgaaact cagatcaagc tttctccctg ctgaataatc
      601 gctgtaatct gcttctcccc actctttttt aagttcgaca cccaaagtga gttcatacca
      661 gtttgcgggg ataatggcgt tttcccagtt cagaacacct gaaacggcag aataggaact
      721 gtcaagatca cctgtcactt ttccgtgttc agcactgtca gaatcgaaaa gggttccgta
      781 aaaaatagaa aagtcggttg tcattgtcca ttttttgtca aaaaagctcg aaacaagacc
      841 gcttttcatt aaaaagtctt cagaatccag atatctgtca gggtcatcct gtccgatccc
      901 cggtccgtca tctatctcat ttttcccttt tgccaccctg aaaataaggt cccactcgtt
      961 gtcattatca attgtgactc ccgttctgaa atatccgctc ccttttataa ctttgtcatt
     1021 ttcaggggtt ttgtcataaa gcccttcttt aactccggaa tatgaatcgg ccattgatat
     1081 tccttcagta tagaaaaaag atgccccgcc gttgtagtaa gctttacagt tgccacccat
     1141 tgaagaaatg ttcgcattaa aagtgtcggg gacatatctt tcattgctca tgtcaagaac
     1201 tgctgttgaa aaagaggtcg aaaaagacgg tttaccgaaa cccttctttg tctgaatgtt
     1261 taaaactccg gcaagtgccc cgcttccgta aagaaccggc tgaggacctt ttagaagctc
     1321 aattttcgca atgttttcag gacttaatga aaggtctgtc gctccatcgg ggctcaacgg
     1381 atttctgagt tcaactccgt caagaagaat taacacatcg cttgatctga agcccctgac
     1441 aaaaacgaac tgtgcctgcc ctgttctccc atctccactg acataaaccc ccggcatctc
     1501 tctgactgca tctcctgccg taactgactg tttttcttcg atttcttttg ctgtaacagt
     1561 tgaagatgcg cttactgaat caatctgtcc gtttcttgat cctgttacaa caacagagtt
     1621 ttcggctgca taaatattat aaaaacaaag tgttaaaagt gagacggcaa atctttcaat
     1681 ctgcaatttt tccctccgcg gggtactaat tgactgcgtc tgagcttgtg tgtctggctt
     1741 agcgatatcg ccttacagtt gcgggacagc accggacttg caccggattt ccacaggaac
     1801 tcaaactgag gttgatgcta accttaaatt caataaagtc aagattggaa agtgttatcc
     1861 gttccttatc ttcagcacaa caactataga ggtaagaacc acagtaaccg tatttgccag
     1921 aattatcggc aggtcgcgta tcatgatccc gtagataagc cacaatacta tgcctgtcgt
     1981 aaatatcaga tacattgaaa gagaaatgtc cttggtctga cctgttttta tcgttttcaa
     2041 cacctgcgga acaaatgaaa ctgtcgtgca tattgcggcg gcaatcccta ttaaagaaac
     2101 aatgctcata acacaactcc gcaaaaatct gaacgaatga tacttatcta atcttttttg
     2161 tccaatggat ggtttatgca gcatttaatt caatcattta ttttgacatt ttaaccgtaa
     2221 caatttaaaa taactgctga attattttag attgaggttg gaatgaaaga ctcaaaaggg
     2281 ttttcagtaa ttgaacttat gatagtcatc tctattctta acatccttgg aactattgcc
     2341 attgcgggct acacagatta cacaaagaaa gcgcgtgttg ccgaagctcc tctgatgctg
     2401 aaatcgcttg ttatcagaat tgatgaatac atgaaggatg cacctccgaa aagtcaggga
     2461 aaatgtgaac tcgttgacag caacggaaga tgtcaggata actttccgaa aaatgctgaa
     2521 gatacaggtt tcagaactaa cagagattca gttttcggca acttttttga atttaaagtt
     2581 aaaactcccc acccgacagg atgtggagaa aaattttata acagatatgc ctatgccctt
     2641 cctcttgtca ataacgatat ccctgaagat taccagttca tgtgtatgaa cgaacggttt
     2701 gatttttttc acagttccaa atcttcagat ccgggaaaac ccgccggcaa aggcagtggg
     2761 aaaaaataat tctgatattt atttacaggc gttagtacac tgatgacagt attcagacag
     2821 cgttgacccg tctatattat aaaaatcatc acaattaacg gctttcaggc acgacttgca
     2881 gttttcgaca agctccatat cacaacttcg gacatttttg aaaaattccg acattatgct
     2941 tatgccgata ccgtgacatt ttgcaagcac atcttccgga acagttgtcc tgcagatctt
     3001 gaacctgttg cagtattcca ttccgagctg atcgaccact tcctttgatt cttttgaaaa
     3061 accgtatctc tcttcaggaa agaaaaggag atatacattt accgcagatg ttatcaccat
     3121 gagcattgca aaaagaatga ccgccagaaa cccggacctt tcctgtcttt cctttttgat
     3181 gagatctttt tcactgatct caaggtcatc cttcttttta tttaactttt ccttgatctg
     3241 ttttgcattt ttagtccctt ttctgatcaa ttccgccatc tcaggagatt cggcatgatc
     3301 aacaggcaga gttccgttta aatctttgtg gttaaggtct gtccccgccg caataagact
     3361 caggacaatg tctttatgat tgaacctcag tgcactaata agagccgact gcccttttga
     3421 attaagctga ttgacatcca ctttgagctt tatcatgaaa tcgacgatat gcgccatccc
     3481 ttttgatgca gcgatcatca gcgctgagta gttattctgg ttctttgcat ttacatcggc
     3541 ccctttattc atgagaagca gtgctgtttc gtcgtatccg ttctgaagag caagcatcaa
     3601 tgcagtcaca ccgttcccgt tgtgcagatc agattttgca tgattggaaa gcagaaattc
     3661 aaccgcttca tagttcccta tcattgatgc aagcatgaga taagtcattt catagtcata
     3721 aagtaccggt ttcaatccgg ttttaaatga tctggcgcct ggtttttcag caaggtcatc
     3781 ttctgttgtc cctgatatta ctgaaagcat tgaaggagtc agatttgctg aaatatctgt
     3841 gctgaacatt ttgtttattt catttttctt aaacagtttc tcaaaaaatt cgcttcttcc
     3901 aagttttatg gcatcaataa atgtttcggg ctcaatttct attattgcac ctgagctgat
     3961 aagtttttcg atggaagtaa ggatgtctat ttcgtgtcga tgatccagga gtttgatcaa
     4021 atttcttact ggaagttttc ccccttcttt ttttattgct tcacaaattt cagcatcaat
     4081 tttcttccct ccggtcctgt caacatcttt tgcaacaccg atgacggcaa aaggcgacag
     4141 ttttttagta tagaattcca caatgaaaca tgattcggcc tttccgaagc agtgtgcata
     4201 aattgaattg tcaggatcct cagagtggag tttctggtaa atggtaactt cttttctata
     4261 tttgtcttca tctgcatcta tctgaaactc tatgctcagg gatttgtctg aaatctgctc
     4321 atcaattgca acatgctgta ccgatttctt gctcttaccc gactcattta caagctgaat
     4381 gactttttct cttgataagg gagccccggt ccctttaaca agaatgccca tgctgcacaa
     4441 ctggtagaaa catttaaaaa gcaaatagtt gtgaggggtc ataatcgaca gaatttcatc
     4501 aattgtcttt cctgcaccca gatagttaag aatgatctcg tgctgatctg tgatcttgtg
     4561 atccttaatt tcatcatgtt caattttagg tctggacccg agcgtcggta tcaccgaaag
     4621 aacagccctg aattcagcta ctctttcctt cagaccggtg gaaatttctt caacagatat
     4681 ttcaacatcc actttctgat cttttgattt ttcgcttact actgaatata aacactcgct
     4741 ccatcttgag gcaatgaata tcgtatcaag tattttttca taaataagct gcttcagtat
     4801 ttcaggagtt gcagctttca taacatcgtc catgcttttt ttagggtcat aacccgcttt
     4861 atacgattct tcaaactgat ctgtatctat gactccataa gaaataaggt actgtcctaa
     4921 ataatcggct ttgctgcttg atgcggcacc agtcaatatt ccattcctga aaaaaagctt
     4981 tttccattca tcaccgttct caaaaaatag atacccttcc tgattttcct tcagtgtctt
     5041 tcttatcaat ttctttactg tcgtatcctg cagcagaaac tttcccattg tcatacctct
     5101 gtaaattttc cactttaaaa aactgatcgg ggcattttac tgtttttttt aaatctaatc
     5161 aattttcgtt tttatttatg ggtttttgta atttattttt tcttaaacca ttcaaaccac
     5221 ttaagcacat tcaccacata ctgttttgtt tcagggtggt cgtatattct gccggctaaa
     5281 aatccatctg tggttgtcgc ctttgtttcg gttgagtggg ttccgccgtt gtatccggaa
     5341 agagatttta tgagatcgcc tttattgtat gtgagaagtg ccgcaaggaa tttgaccgag
     5401 cagtttatcg caagttcagg gtctttcagt ttgctgtatt cgcatttgag cagttttgca
     5461 gtttgaggca ttatctgagt cagtccataa gctccggcgt gactttttgc ctcaggatta
     5521 ccacgggatt cctgataaat tattgcttta acaagtcccg ggtgcattgt tactttaacc
     5581 ccggatttat tcatgtatgg atatttagca accgactttt caataattgc tgaatatttt
     5641 tgatttatca tgttcgcagt gggaatgtga atctggctaa gcagtaaaaa agaaatgagg
     5701 aagatcattt tttctctttc agtccgaaga tttcagatgg tttattccgc tttttctctt
     5761 cgctttcgag tttctttttc acttcaagat aggcatcgga catatctttc aggaactgtt
     5821 ccttcatgat cgcaagatcg ctgttcttct taagattctc aaggagttcg accgaaatat
     5881 attgagcttg catcagattt gcaagtccga gtgcctcaat gttgtcgttt gattttgcct
     5941 gttttaagta ttcatcgtaa taatttttaa atgtgttctt cctgacgatg tcatttttca
     6001 tttcatattc gagtcctgat tcgatccacg ccttgtggat cacctttttt gcactctccc
     6061 cttttgtaaa accatccttc atcttttgtg cggcaggata aagttcaggg aacataacat
     6121 gattgtcata attttttaaa agcgattcat gataattctt cacctcacca ctccattttg
     6181 aaacataatc ggagtacttg ccttttatca tttcatgtcc gcccttaacc attccagcca
     6241 tctcattttt gagttcagaa tattcaggca tcacctcgtt aatgtgtttt tcaacatctg
     6301 caagccattc gtcagccgat ctttctttaa gctctctgcc catcttggaa atgtccctga
     6361 tagtcgaagt cgtgtttttt attgaagcga ttacctcaac aaactgtgaa atatattcga
     6421 cataatgctg atactctttt attctctgct ggatcgcaag atttgacatg agcagcgttt
     6481 gcgtctcgat cgcagcatca gttcccggac ttgcaaccat aagttgcgca gaaagatcga
     6541 gggacaggaa taatccggcg attatcgcaa gcaattgttt tttcatttga cctccttcgt
     6601 caattttttc taatatatga tagatttaaa gtatcggcaa gttttctgtc cgttttattt
     6661 ttttttgcaa aatttcacta ttatgattta attttccgga atttttgaac ttttgaggta
     6721 aaaatgaaaa atctttcagt tttttttatt gtgcttatca caatgttcct tgcttcctgt
     6781 accgacacat cacttaagcc ggaagaagcc gaacggctga taaaggagcg gctttcaaaa
     6841 atgccgggca gaagcgtttc gttctatctt ggtgccatga cagaccccgc ttatcttccg
     6901 atttataaaa agatcgccac aggcaaatat cttacactta aagaagatgt ctttgttgaa
     6961 gcggcaggaa aaaaaatgcc catgttcgaa gccactccgg aaggggaaaa gatctttaaa
     7021 tgtgacaaca acaggtgctc tgtggaagtt tgcaaaacgg agtttgacaa gttcgtttcg
     7081 atagtgaaca aaggtaaatt tgcaaattca acctatcagg tcaagaccat ctgtgacgga
     7141 gaaatataca atattttcag gccattagct gacaggcaat atataaaacc cgagactgtg
     7201 gaagaaaagg cagaattcga atattctaaa gatacatgga tcataaaata gcatttttca
     7261 tctaacaaaa aattccttgc tttcctgtta ttagtaagtt ttaataacaa cggtgttgat
     7321 gtttaatttt ggaggaaaat atgtatcatg atcttgaaaa atctttttca aaaatgacaa
     7381 aaagagccaa aaaatcagcg atcagagagc ttttgaagct taccaacaaa ccgggactga
     7441 tctcctttgc gggaggactt cccgccccct gcacattccc ttcagaggaa cttaaagaga
     7501 tcacaaatga agttctcaca aaagaagcaa atatcgcgct ccagtacgga acaaccgaag
     7561 gtgatgacct tcttcgtgaa cagcttacaa aaagatatca gtcaatggga cttaacatca
     7621 caaaggaaaa tcttgtgatc actacttcaa gtcagcaggc acttgacttt ctgggaagag
     7681 ttttcatcaa cgaaggagac aaaattattg ttgaacttcc aagctatctc ggcggacttc
     7741 aggcattcaa tgctttcggc gccgacatgg aagggataaa gtcggatgaa ttcggaatgt
     7801 gtccggttgc acttgaaaaa aagatggctg aactctcgtc tctggggaaa aaaccgaagt
     7861 tcatctatgt cattcccgat tttcagaacc cgtcaggaat tacgatgccg gaatccagaa
     7921 gaaaagagat cattgacatt gctcacaaat atgaagttct cattgttgaa gacagtccct
     7981 accgcgaact cagatttgat ggcgaaccgc agaaaacgat ataccagctt gacgaaatgg
     8041 gaacgaccat aactcttgga actttttcaa agatcttcgt tcccggattc aggatcggat
     8101 ggataatcgc acatccggca atagttgata agtttgtcac cacaaaacag tctgttgatc
     8161 tgtgcacacc tgcatttgtc cagagaattg ccgcaagata catggaaaaa ggatattttg
     8221 aaaagaatct caaaagcaca ataaaacttt acggcagaaa aaaagaagtg atgatggaga
     8281 gctttgagaa atatatgccg gaaggtgtca catggacaaa tcctgaaggg ggacttttcc
     8341 tttttgtcta tcttcctgag cacatggatg ctcagaaact tttcctgacc gccgtagaaa
     8401 ataatgtcgc atttgtaatc ggtcaggttt ttcactgtga cggaagcgga ataaacacaa
     8461 tgagaataaa tttctcgttc tgtgacgatg aaaagatcgt tgaaggtgtg aaacggcttg
     8521 ccgctgctat taaggaatat aaaaactgat cattttttct gtgaaagttc ggaaaccttc
     8581 tcaaagtaaa tcttccaaat aatgatgaaa tacgatatac ttccgacgca ctcaatcgta
     8641 ggata
//
LOCUS       DAOOYB010000113         8609 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_9086_length_8609_cov_9.701076, whole genome shotgun sequence.
ACCESSION   DAOOYB010000113 DAOOYB010000000
VERSION     DAOOYB010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8609
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9086_length_8609_cov_9.701076"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1642)
                     /locus_tag="PL056_07840"
     CDS             complement(<1..1642)
                     /locus_tag="PL056_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46965.1"
                     /translation="MKKVLIIALVLFFVIVQSCVKDENENESGDSGNSGNSGNSGNTG
                     DSGDTGNSGDTGDSGNSGDTGDTVLDCGCDVASDDDDNDGISNGSEGCEDVDGDGLPN
                     CLDLDSDGDGTADIEECPSIPCKDTDSDEVADYLDRDSDNDGIKDKKEKEDGTDPYNK
                     DTDGDGSDDLAEIVYGSDPKDPNSKIPDGLFYVVLPYSAPDKVHRKLDFNTNITKVDV
                     AILLDLSGSMGQELDNLKAEIKTKIIEDLPKEIPGMTVGFSIAHFMDWNKQKMDEVYK
                     VDQYVTTDPVEAQNSLDNMPGTSGGTEPHEETLYQAASGAGLFNALLNQPPLGAGTFN
                     LAPVDCTGKEGNVGGMCFRELAMPLFIMITDEDFSHIPVGKQGFGEEYWDPASPGHTL
                     EDAIIAMNGINAKFIGIDSGFSCEVDANYNCVPGTEKEANYAEEDYKIIAEGTASLDK
                     NGDPFLYHTANADGSGMSDQIADAVVALTTYVQMDVTTSTSSDEECDGVNVAAFVVES
                     KPNEATPPEGVASKDDTTFYKVEPGTLVSFDVYFKNTFC"
     gene            complement(1657..3072)
                     /locus_tag="PL056_07845"
     CDS             complement(1657..3072)
                     /locus_tag="PL056_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46966.1"
                     /translation="MKKAILIFLVLSASCIFGADFTTYYSDKSSKSVASLEFVKKLAE
                     KYPDINFNSINVTESSETKKSFKELIERFEMEKAVVPLFVIGDMREAGYEPDSAEEYQ
                     QKIEQMIKKFIAGSSDATFYFFYSHKCPHCKKARPLVDAWEIKFPQIKFKRLEVARDK
                     TNRELFEKICTDLKINNPGVPTFTLGGSYVTGYEVGEHEELIEKMITEYLAGNPDAVK
                     NASSDTTVDVPFLGKLDASLISLPSFTLLIGLLDGINPCAIWVLMFLLTLLVNTKSRK
                     KLIMTGTVFVVASGVVYFLFMTAWLNIFLFMGITETVTILLGVVAIIMGLINLKEFFF
                     FKKGVSLMIPESAKPKLFKKMRGVMGTDNIFLGLAGTIALAFFVNLIELGCTIGLPAI
                     YTRVLSISDISTLQKYLYMALYNVYYVVPLAIIVLIFVFTMGKYRFEEKYAKILKVVS
                     GILMLTLGLILVIKPELLIFI"
     gene            complement(3074..3796)
                     /locus_tag="PL056_07850"
     CDS             complement(3074..3796)
                     /locus_tag="PL056_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46967.1"
                     /translation="MRIFAFYLLSMLFTASCADTEVIYKDSELFSDNDELSECPDDMV
                     EAGRICIDRYEASRKDATAEDQGTATDIAISKKGVLPWMENPMNNAAYEQFKFACVAA
                     GKFLCRDSEWVSICEGPEKLTYSWGNNWDVEICNNVDTFCDDHCLENDIPESECSTFP
                     NCGYEYYCFKPALTGEFQDCVNFAGAHDINGNVWEVTDTGSGYKIRGGAFNCAGAIDR
                     LECTYSAGWSELYAGFRCCRER"
     gene            complement(3806..4792)
                     /locus_tag="PL056_07855"
     CDS             complement(3806..4792)
                     /locus_tag="PL056_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46968.1"
                     /translation="MQSLRKCLLLIILIIFTNSYADENDISWSGSFSTGTGYDSYIPS
                     LGRDSTKRIGSKELLSDIGINLDIYDFSIGTSLFVNGLVADKWRQSQLTGMINAGWSS
                     TFGEYDLSFSIYGTFAGYDFDDFRSYYTDWSGDGEIFFNHSDNMSFFFSITGGYTHGI
                     DSTLKYLRGPYAGFSTGEYFYFGEFDSYVRIYGNANFNFYDDISGSSYPYLGESGISS
                     LSGKNRGIDATVAVRGRFNYEKLFFIFTPTYRNSTMFDKDVWEYSDRIVKKRRVDHAL
                     LLEFEPGWSPDEDLTVSIYYLLEKNFSTLGKNDYTDENYIRHNIRLLINYDF"
     gene            complement(4780..5019)
                     /locus_tag="PL056_07860"
     CDS             complement(4780..5019)
                     /locus_tag="PL056_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46969.1"
                     /translation="MKKILFSVIAIFFFLNPVCADEKQPEKSGEKENKGTDTVDLDDD
                     SDDSKAEERKKEREKEKKKEKKDKKKKGKKGKCKV"
     gene            complement(5036..6022)
                     /locus_tag="PL056_07865"
     CDS             complement(5036..6022)
                     /locus_tag="PL056_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46970.1"
                     /translation="MKRSACIISFGNRESQTVIESAFSRLEIKTETVLIKKCIHMADI
                     SDYFISSTGELSPLEKGFLKDGKKDQSAILAIQKNSIDKIGNLLQKETLFFIEDDFSP
                     ESFYIKKIFFRNRVPFFSFGSILPDGDISIVPGSLDIGFNITLLKNYRCQPSSVMPQY
                     PVFNGNIAIEAPPDTIPLRYLLHFRSSPFLVRNIIAALNKFYGNERHPAVSVQDPVRP
                     YNSYSASTRAFQIKNLLGKRGCGFENLSDLPAGTTVLTWSTKRFFALLKAGMRPVPLT
                     KCLVSSIFSSRKTTPEELIFSDPAQHDVDRIYCFVKETGVENLAEMVYKLKS"
     gene            6103..6963
                     /locus_tag="PL056_07870"
     CDS             6103..6963
                     /locus_tag="PL056_07870"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HPM46971.1"
                     /translation="MKRFIFILFFILAGCSGKKEKVRTDDPCSKIVSTAPSITETLFE
                     LGLGDRTVGVTDNCKLPQDAVDIKRIGKVLDVSSEAVLSLNPDTAIVLSANESLAVKM
                     NNLKINTIVVDQSTIEGFVFSLKIIGEVCSVQQSADDLNRSIEEKLNSVLSSFKPDKS
                     LKIMVVAGRDYFDEGIKDVYIAGRDGFYEKIMEIIGVQNVYQGSLAYPKIQVEGIMTL
                     DPDMIIDVVTMENISGEKLASIQKNWDKLKNIKAVKNGKVFVVNKDYWSIPGPGYVRI
                     VEDFIEMIKK"
     gene            6960..7739
                     /locus_tag="PL056_07875"
     CDS             6960..7739
                     /locus_tag="PL056_07875"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPM46972.1"
                     /translation="MTLIIKSLNVQIDGFKIIKNISFSASLGENISIIGPNGAGKTTL
                     LRAVAGLVDFKGTVFINGNDTGKIDILKRAALVSYVAQLSDIAGDITVRHFLELSRFH
                     SLKPWASLTVLEKRLIENALNVTQTKEFEKREMSTLSGGERQRVLIAGAIVQDTPLIL
                     LDEPLNYLDPVQRDKVSKLIRKIADAGKTVITVTHEINEAFLFSSRIIVMADGSIAAE
                     GSCEDIINSDILEKVFSTSFHKLTHPETNRPLMFPKGSGNE"
     gene            7732..>8609
                     /locus_tag="PL056_07880"
     CDS             7732..>8609
                     /locus_tag="PL056_07880"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HPM46973.1"
                     /translation="MSKPLIKLTILFVSAIILLWILPFAGMNTVAFSSEIFQTIRLPR
                     AIMSFAAGAVLGVCGMSFQSMFRNDLATPYTLGISSGAALGAVIAIKFISCASFCPLF
                     SFVQIFSFIGAIATVALIYSVVKAKERMNTDFLLLAGVAVNFTFSGLILFIQYLASGS
                     ESSMMIRWMTGSLDITGFEPVLKIIPALIIFPVSLLLHRELDLMRINSDIAASRGVDL
                     KKTGNTIFFAVSFAVAVVVSQTGPIGFVGMIAPHIGRKLISKEHKYLAVASFMIGGMI
                     LLIADTISRTVIAPSE"
BASE COUNT         2423 a         1778 c         1775 g         2633 t
ORIGIN      
        1 tgcagaatgt attcttgaaa tatacatcga aagatacaag tgttcccggc tcaactttat
       61 agaaagttgt gtcatctttg ctggcaacac cttcgggcgg agttgcttcg ttgggttttg
      121 attcaaccac aaatgcggcg acattaacac cgtcgcactc ttcatcggac gatgttgaag
      181 tggtaacatc catctgtaca taggtggtaa gtgcaactac agcatcggct atctgatcgg
      241 acattcctga tccgtcagca tttgcagtgt gataaaggaa agggtcaccg ttcttgtcta
      301 atgaagcagt cccttccgcg attatcttgt agtcttcctc agcgtaattc gcctcttttt
      361 ctgttccggg aacacagtta taatttgcat caacttcaca cgagaatccg ctgtcgatac
      421 cgatgaactt agcgtttata ccgttcatgg cgatgatcgc gtcttcaagt gtgtgtcctg
      481 ggcttgcagg atcccagtat tcttcgccga atccctgttt gccgaccgga atatgtgaaa
      541 aatcttcatc agtgatcatt atgaaaaggg gcattgcgag ttctctgaaa cacattccgc
      601 cgacatttcc ttcttttcct gtgcagtcaa caggcgcaag attgaaagtt ccagctccaa
      661 gggggggctg attcaaaaga gcattgaaaa gtcctgcacc tgatgccgcc tgatagagtg
      721 tctcttcgtg cggttctgtt ccgccgcttg ttcccggcat gttgtcaagc gagttttgtg
      781 cttcgacagg gtctgtcgtt acatattggt caactttgta aacttcatcc attttctgct
      841 tattccagtc catgaagtgt gctatcgaaa aaccgactgt cattcctggg atctctttcg
      901 gcaggtcttc aattatcttt gttttaattt cagctttgag attatccagt tcctgtccca
      961 tcgaaccgga aagatccaga agtattgcga catccacttt tgttatattg gtattgaaat
     1021 cgagtttgcg gtgaactttg tcaggagcgc tataggggag aacaacataa aaaagcccgt
     1081 cagggatctt gctgttagga tctttagggt ctgaaccgta aacgatctca gcaaggtcat
     1141 cacttccgtc cccgtcagta tccttgttgt aaggatcagt tccgtcctct ttttctttct
     1201 tatccttgat gccgtcatta tcgctgtcac ggtcaagata atcagccact tcatctgagt
     1261 cagtatcttt gcagggaatt gaagggcact cctcgatatc tgctgttccg tccccgtcac
     1321 tgtcaaggtc aaggcagttc ggaagtccgt ctccatcgac atcttcacaa ccttcacttc
     1381 cgtttgaaat gccgtcatta tcatcatcgt cagaagcgac atcacaaccg cagtcaagga
     1441 cggtgtcacc cgtatctccg ctgtttccgg aatcacctgt atcaccggag tttcccgtgt
     1501 cgcccgaatc tcctgtgttc cctgaattac cggaattacc tgagtttccc gaatcacctg
     1561 attcgttttc gttttcgtct ttcacacatg actgcacaat cacaaaaaaa agaaccagtg
     1621 caatgataag aacttttttc atttttcacc tcgtatttaa ataaagatca gtaattccgg
     1681 ttttatgaca aggataagcc caagtgtgag cattagaatt ccgctcacta ctttgaggat
     1741 cttggcatat ttttcctcaa agcggtattt gcccatcgta aagacaaata tcagcacaat
     1801 tattgcaagg gggacaacat aataaacatt gtaaagggcc atgtatagat atttctgaag
     1861 agttgaaata tccgatattg aaagaactct cgtataaatt gcaggaagtc cgatcgtgca
     1921 tccgagttcg atgagattta caaaaaaggc gagagcgatc gttcctgcaa ggccgaggaa
     1981 gatattgtca gttcccatta cccctctcat ttttttaaag agtttcggtt ttgcgctttc
     2041 ggggatcatg agcgacacac cctttttaaa aaagaaaaac tcctttaagt taatgagacc
     2101 cattataatt gcgacaactc caagaaggat tgtaaccgtt tctgttatcc ccatgaaaag
     2161 aaaaatgtta agccatgcag tcatgaacag aaaatataca actcctgacg caacgacaaa
     2221 aacagtgcct gtcattataa gcttttttct gctttttgtg ttgacaagaa gagtgagcag
     2281 aaacatcaga acccatatcg cgcaagggtt tatcccgtca agaagtccaa tcagcaaagt
     2341 gaaggagggg agtgatatca gtgacgcatc aagtttccca agaaaaggga catcaactgt
     2401 cgtgtcgctt gatgcattct ttacggcatc ggggtttccc gcaagatatt cggttatcat
     2461 cttttcaata agttcttcgt gttcacccac ttcatatcct gtgacatacg agccgcccag
     2521 agtgaaagta ggcacaccgg ggttgttaat tttaagatcg gtacatatct tttcaaacag
     2581 ctctctgttt gtcttgtcgc gggcaacctc aagccgttta aattttatct gaggaaattt
     2641 tatttcccag gcatcaacaa gcggtcttgc ctttttgcag tgagggcatt tgtgtgaata
     2701 aaagaagtag aaagttgcat cgcttgagcc ggcaatgaat ttttttatca tctgctctat
     2761 cttctgctga tattcctcag ccgaatccgg ctcataaccc gcttcacgca tatcacctat
     2821 gacaaaaagg ggaacgaccg ccttttccat ctcgaatctt tcgatcagtt ccttaaagct
     2881 ttttttagtt tcagaacttt cagtcacatt gatgctgtta aaatttatgt caggatattt
     2941 ttcagcaagt ttttttacaa attcaaggga tgcgacactt tttgaacttt tatcggaata
     3001 atatgtggtg aaatcagctc caaatatgca actcgctgat aacactaaga atataagaat
     3061 tgcttttttc atttcatctc tccctgcagc atctgaaacc ggcgtaaagt tcgctccatc
     3121 cagctgaata agtgcattcc agccggtcaa ttgctccggc acagttgaaa gcaccgcctc
     3181 ttattttata tccgcttcct gtatcagtca cttcccagac atttccgttt atatcgtgag
     3241 ctcctgcaaa attgacacaa tcctgaaatt ctcctgtgag agccggcttg aaacagtaat
     3301 attcgtatcc gcaattcggg aatgtgctgc attctgattc aggaatatca ttttcaagac
     3361 agtgatcatc acaaaaggtg tcaacattgt tacatatctc gacatcccag ttgtttcccc
     3421 atgaatatgt cagcttttca ggtccttcac aaatacttac ccattctgaa tctctgcaca
     3481 gaaactttcc tgccgcaacg caggcgaatt taaattgttc gtaagcagca ttattcatag
     3541 ggttttccat ccacggaagc actccctttt ttgaaattgc gatatctgtt gcagttccct
     3601 gatcttccgc tgtggcgtct tttcttgaag cttcatacct gtcaatgcag atcctgcccg
     3661 cttcaaccat atcatcagga cattctgaaa gttcgtcatt atctgaaaaa agttcactgt
     3721 ctttgtaaat gacctcagta tcggcacaac ttgcggtaaa aagcattgat aataaataaa
     3781 aagcaaagat tctcatatgt taacctcaaa aatcgtagtt tatcagcagc cgtatgttgt
     3841 gtctgatgta gttttcatct gtgtagtcgt tttttccaag agtcgagaaa tttttttcca
     3901 aaagataata aatgctgact gtaagatctt catcagggct ccatcccggc tcaaattcaa
     3961 gaaggagagc atggtcaaca cgcctctttt taactatgcg gtctgaatat tcccagacat
     4021 ctttatcaaa cattgtgctg ttgcgataag tcggggtgaa tatgaaaaac agtttttcat
     4081 aattaaaacg acctcttact gctactgttg catctatgcc cctgttcttt ccggaaagtg
     4141 atgatatccc tgattcacca agatatggat aacttgagcc ggaaatatcg tcataaaaat
     4201 tgaaattggc attgccgtaa attcttacat agctgtcaaa ttcgccgaaa taaaaatatt
     4261 caccagttga aaaaccagcg taggggcctc tcagatattt cagcgttgaa tcgataccgt
     4321 gagtgtaacc gcctgttatt gagaagaaaa agctcatatt gtcagagtgg ttgaaaaaga
     4381 tctcaccgtc tcctgaccag tcggtgtaat aactccggaa atcatcaaag tcgtatcccg
     4441 caaaagttcc gtaaattgaa aaagagaggt catattcacc gaatgtcgag ctccatccgg
     4501 catttatcat acctgtcagt tgggattgcc gccacttatc tgcaaccagt ccgttaacaa
     4561 aaagagatgt tccgatcgaa aaatcataaa tatccagatt tattccaata tctgacagga
     4621 gttcttttga tccgatcctt ttggtcgaat ctctgccaag agatggaatg taagaatcat
     4681 aacctgtacc tgttgaaaag ctgccagacc atgagatatc gttttcgtcg gcgtaactat
     4741 ttgtaaaaat tatcagaatt atcagaagta aacattttct taaactttgc actttccttt
     4801 ttttcctttt ttctttttat cctttttctc ctttttcttt tctttttccc gttctttttt
     4861 tctttcttcc gccttggaat catcgctgtc gtcatcaagg tctacagtgt cggttccctt
     4921 gttctctttt tcgccgctct tttcgggttg tttctcatcg gcacaaacag ggttaaggaa
     4981 aaaaaatatt gctatcacgg aaaaaaggat ctttttcaaa gtttctctcc ttagttcaac
     5041 tttttaattt atataccatt tccgcaagat tttcaacacc tgtttctttc acaaaacaat
     5101 atattctgtc cacatcgtgc tgtgccggat cagaaaagat cagttcttcc ggcgttgttt
     5161 ttcttgaaga aaatattgag gaaacaaggc atttagtcaa tgggaccggt ctcattccgg
     5221 ctttaaggag agcaaaaaat ctttttgttg accatgtcag aacagttgtt cctgcgggga
     5281 gatcggagag attttcaaaa ccgcatcctc tttttccaag caggttcttt atttgaaaag
     5341 ctctagttga agctgagtat gaattgtaag gtcttaccgg atcctgaaca gatacggcag
     5401 ggtgtctttc atttccatag aatttattaa gtgcggcaat tatgtttctg accaggaaag
     5461 gtgaagatct gaaatggaga agatatctca aaggaatagt gtctggcggg gcttctatgg
     5521 caatatttcc attaaaaaca ggatattgcg gcatgacaga agagggttgg catcggtagt
     5581 ttttaagaag tgttatgtta aatccgatat caagtgaccc cggaacgata gagatatctc
     5641 catcaggcag aatgctgccg aaactgaaaa acgggactct gtttctgaaa aagatttttt
     5701 taatgtagaa actttcagga gaaaagtcat cttcaatgaa aaaaagtgtt tctttctgta
     5761 gaagatttcc gatcttgtcg atggaatttt tctgtattgc aagaattgcg gactgatctt
     5821 tctttccgtc tttgagaaat cctttttcta aaggggacaa ctcgcctgtc gaagaaatga
     5881 aatagtcaga aatatcagcc atatggatgc atttttttat aagcactgtc tcggtcttaa
     5941 tttcaagacg actgaacgca gattcaataa ctgtctgtga ttccctgttg ccgaaagaaa
     6001 ttatgcatgc cgaacgcttc atggggtgaa taatatcgat cacccttgca taagcaaatt
     6061 attttgataa tatcagacag tatttttgag gattgcaggc aagtgaagcg ttttatattc
     6121 attttatttt tcattttagc tggatgttcc ggaaaaaagg aaaaagttag aaccgatgac
     6181 ccttgcagta agatcgtaag cactgcgcca agcataaccg agactctttt tgagctcgga
     6241 cttggggaca gaacagtggg ggtgactgat aactgcaaac ttcctcaaga tgctgtagat
     6301 attaaaagaa taggcaaagt gctcgatgtc agcagtgagg cggtcttatc cttaaaccct
     6361 gacacagcga tagttctcag tgctaacgaa tctcttgcag tgaagatgaa caatcttaaa
     6421 ataaatacga tcgttgtaga tcagtcaaca attgaaggtt tcgttttctc tttaaagatc
     6481 attggcgagg tttgctctgt tcagcaaagt gcggatgacc tcaatcgcag catcgaagaa
     6541 aaactgaata gtgttctaag tagctttaag ccggataagt cgctgaaaat aatggttgtt
     6601 gccggcagag attattttga tgaagggata aaggatgtct atattgcagg tcgtgacggg
     6661 ttttatgaaa agattatgga gatcatagga gttcagaatg tttatcaggg ctctctggca
     6721 tatccgaaaa ttcaggttga aggaataatg actcttgatc ccgatatgat aattgatgtt
     6781 gtaacgatgg aaaacatcag tggagaaaag cttgcaagta tacagaaaaa ctgggataag
     6841 cttaaaaata ttaaagcagt gaaaaacgga aaagtgtttg ttgtaaataa agactactgg
     6901 tcaattccgg ggccgggata tgtcaggatc gttgaagatt ttatagagat gataaaaaaa
     6961 tgacattgat tataaaatct ttaaatgtac aaattgacgg ttttaaaatt ataaaaaata
     7021 tctcattctc agcgtcactt ggtgaaaaca tctcgatcat aggtcccaac ggagcaggaa
     7081 agacaacact tttaagggct gttgcaggac tggtcgattt caaagggaca gtttttataa
     7141 atggcaatga cactgggaaa attgatatcc taaaaagggc ggcacttgtt tcttatgttg
     7201 cgcaattatc tgatattgca ggtgatataa cggtcagaca ctttcttgaa ctttcaaggt
     7261 ttcatagttt gaaaccttgg gcaagtctga ctgttttgga aaagcggctc attgaaaatg
     7321 cactgaatgt cactcagaca aaagagttcg aaaaaaggga aatgtcaact ctgagcggcg
     7381 gggaaagaca gagggttctc attgcaggtg cgattgttca ggatactccg ctcattcttc
     7441 ttgatgagcc gcttaactat cttgatccgg ttcaaaggga taaagtgtcc aaacttatca
     7501 gaaaaattgc tgatgcaggc aaaactgtaa taacggtaac acatgaaatt aatgaagctt
     7561 ttttattttc ttcaagaatt attgttatgg ctgacggcag tatcgccgct gaagggagct
     7621 gtgaagatat tataaattca gatattcttg aaaaggtttt ctcaacttct tttcataaat
     7681 taacccatcc tgaaacaaat agacctctta tgttcccaaa agggagcggc aatgagtaag
     7741 ccattgataa aactgacgat tctgtttgtg tcagcgataa ttcttttgtg gattctacca
     7801 tttgccggaa tgaacactgt agcattttcc tcagagatat ttcagacgat acggcttcca
     7861 agagccatca tgagctttgc cgccggagca gttctagggg tttgcggaat gtcgttccag
     7921 tcaatgtttc gtaacgacct tgcaacgcct tacaccctcg gcatcagcag tggagcggca
     7981 cttggagctg taattgcgat aaaattcatc tcatgtgcat ctttctgccc tcttttttca
     8041 tttgtccaga tattcagctt catcggtgca attgccacgg ttgctctgat atattcagtg
     8101 gttaaggcaa aagagaggat gaatactgat tttcttcttc ttgcaggggt tgccgtcaat
     8161 ttcactttca gcggacttat tctgtttatt caatatcttg caagcggatc agaaagttcg
     8221 atgatgataa gatggatgac cggctctctg gatataacgg gctttgaacc ggttctcaag
     8281 ataattccgg cacttatcat ttttcctgtt tcgcttttac ttcaccgtga acttgacctg
     8341 atgaggataa attcagacat agccgcatca aggggagtgg atctgaaaaa gaccgggaac
     8401 acaatatttt ttgcagtatc atttgcagtt gcggttgttg tatctcaaac agggccgatc
     8461 ggatttgtcg ggatgatcgc acctcatatc ggcaggaagc tgatctcaaa agagcataaa
     8521 tatctggcgg ttgcatcatt tatgatcggc ggaatgatcc ttctgatcgc tgacactata
     8581 tcaagaactg tcatcgcacc ctcagaaat
//
LOCUS       DAOOYB010000114         8585 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_9124_length_8585_cov_13.768464, whole genome shotgun sequence.
ACCESSION   DAOOYB010000114 DAOOYB010000000
VERSION     DAOOYB010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8585
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9124_length_8585_cov_13.768464"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..2961)
                     /locus_tag="PL056_07885"
     CDS             complement(31..2961)
                     /locus_tag="PL056_07885"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HPM46974.1"
                     /translation="MNKRLTLLFIFLFFVSAEIIAQDIISTVVWDTPLEKDKFSRLIK
                     VKEGDEFSIKNIRRTVKLLYATKKFEQIAVMLEKEKNGVKLILKGSPQLIITDISIKG
                     NSKFSDRQLKSASGTGKNTYFFTSDLERIRDEIIYFYRENGFFNTLVNIATEKTGENS
                     ISLKIVIIEGVQEKINEIRIYGSMNKSEKRKLEYELGVSFKGNPNTDKNINDINEFIN
                     TYFKDRGYLDVNVNTDRLPGGKVSFSINKGLLYILDITGDESFSPDTLKTIITGLSNY
                     HFDAEGIKRKLLIFYQAYGFPDAVIDVDISDLSLRKNDQTRTISVNIDEGERRFINDI
                     VFTGGSSFNRNVVVNRLKDFIEKKIEEENFPQIKITRSQTGGGYKDTDNERVKALKRS
                     KKDKVMLPDSPLGIPEAYLEDIKEIVEKVYLNEGYMDISVLSAEIIDNSEGLYLLVGV
                     KENTRFMLTGVTATTGDPVLDRIMKKKILLPRAIPFNENIVEAYRKRIKEFLSSKGYI
                     FAHITHDLEYDGQKILLKFKAEYLFPVKVTEVVIAGNFITEEWAVKGALRIRPGDTLK
                     GDTFQYRRRDLLQTGMYQSVMINFIDPEFPSEEKDVVVVVSEVERFKISPGIGISSDE
                     GGRIFGSAEWRNFGGSGFSTKFSFKISRKIELFMGDEFKKYYSSDFTLFEQFERKFNL
                     AFLFPDLYLPFLPLSAQVEAFHIHDIRSNAGLPYMIDKNGMYFSFFRRFSNNYFLSTG
                     VEIAYQNERDHELDEAGDVYYNNIERLVISPEFRGYIDFRDSLFFPTKGYKTFFKYAN
                     KTSVYGEATSISYFENNLSLYIPLRYRMNFAGELEPKDTLIYHAFIETAFLIQHSGDL
                     TSDDVLKLGGNTTIRGFFNDELQPNDLENEKSAEGRYSFVMRNELRFKLIESFYLISF
                     FDMGNLWQKLGNIGDGSLFRYTAGGGLTYASPIGALTAQVGFNIRPRENEGIWAFHIY
                     LSTF"
     gene            complement(2954..6673)
                     /locus_tag="PL056_07890"
     CDS             complement(2954..6673)
                     /locus_tag="PL056_07890"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HPM46975.1"
                     /translation="MNRIKFLSITLLFTVSLLFTVKFAFYPVSSFFLDIYGIRYDFSR
                     IDYESGRRIVFEDLSIEIKDMLSFKAGKLTFSAGRKSSGFLQKEYVLHYLELIDGELT
                     FDSKWLLEPSDITFPETFPFFPLEEVFIKNLSVSAMIDGQNGFIAKNIDISGNGKYEI
                     EMADNRYFHSSLKEPVIVDLYANIEAKDFFYAIDNIAVSSKGMVISGEKKGETGNLSG
                     RVAVDLSEAVEIFSQTAKGEVYADYVLDIAGKVPKIDTTLTVSGLNYEGFRPWDIHAF
                     LQITPSTMHIDRLNLFHNDKVFLSLDGVFPYKTKEIKGVVRLYRFDLDDCLRRMTTSG
                     IVNLIVSGKADYVFSTETLSADVKTDLVVNEFDVGKKEILDLPREVFVTGNVTVGADG
                     VKLHDGIVKTKDETSRLIVKDSWFGFADSMKFHIPVVAGSWINLEDVNQITGFNVKGR
                     GSIEALVTSFYENPYITGRFSGTGCSFHGFNAQSCTVDTKLKEFVLRIGVEDIKQNSI
                     SSKDSFVSLDFNDEILPVSFLINDAKGSLKDAAAVFDIQTDAFSGNVALTAEGVYKNG
                     LSKLNASLKADNLKIKGVRAADSFIFKINDKDPVFINGIGNLNYGKSSIAIEGRMSKK
                     DLDTEISAKFSSFFKDDFPLPADLDYEEPSLDIKLAGILDQPDVSAKFEANGISFSGV
                     KLGDLAVSAQLAGENMDFGIKGKLGKSIVFSAMMKEFKPENLSASLKIKDFVHKVSDF
                     FVKLSLNAKMEGENIDAKVTTLMAEKSGFFVRNNAPFSISGKMDDLEIEKAYFDGETA
                     NFFLEGRIVNYSPHISAKGTLFPRMVEILYPGEITGVDGKFYFDVALDKEDIKGELRM
                     VDGSYRLKNPQIIFSGFNGLLSFDKNKWKIENLNGFAGGGRVVLSGEGSMFPFDNAAL
                     KLKLTNLTGKYALTGDFGLSSNLDIIMFGPEQISVSGDIELRNIVYNQPLSLDSDFIK
                     MISKLGRERAGAQIEKTLPIDLSIRVTGKNNIRIKTNLIESDLFIDTTITGTARKPEM
                     SGTILLKNGKIQYKQNDFTIQRGIITFEDGNGINPFIDLESFRNVTAKVVDDEKDFKI
                     IMTARGNPFDGELEVNFDSIPQLDQQQLVSLLLWGNIGDSFSGDIAIAAVTDIMGITT
                     QVRKNFNLTKFELIPKYSEFDDKTVLKLVAEKEIYRNLFLSLESNPSDTTDQIIEIKY
                     KTKSLETILGWKNKDRLESSFGAIGFDFRLEYYFE"
     gene            complement(6689..6764)
                     /locus_tag="PL056_07895"
     tRNA            complement(6689..6764)
                     /locus_tag="PL056_07895"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6729..6731),aa:Gly,seq:ccc)
     gene            complement(6833..7618)
                     /locus_tag="PL056_07900"
     CDS             complement(6833..7618)
                     /locus_tag="PL056_07900"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPM46976.1"
                     /translation="MVISATGISKFFGKKAALDNVTIDIPAGTVFILVGPNGAGKTTL
                     LRILCKELKPSSGIIKTKNGGTVAVAEENRDFFKNFDANKYADMWELLYPGFEREKFF
                     NMMKELKIPADKPLEQYSKGMKTWLHNSLVISSNSEVMIFDEPLQHLDPAVRMKFHTI
                     LHNEALKSRTLIISTHEIDEFDKYALHLALIHNGKVVLCDEVSKILFEHRIVPGTQSS
                     ANMSVIGPVFSEKLVKTKENTGREPLLKEIAAGYINGCDLGSS"
     gene            complement(7612..8181)
                     /locus_tag="PL056_07905"
     CDS             complement(7612..8181)
                     /locus_tag="PL056_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46977.1"
                     /translation="MIKLLNHFEAFLLLETTTRKKTFGLLFLSLLLPGHIKYLFVFIL
                     ISKLLPADVNSRRIKMILSLPFSRSEIFLFAFITGFLIIFSATVIGEAFFIGKTDFAV
                     FTRLFVFYCAYFGILMLVTTKGADNLTFPVFLLITDLIAGNIGTPETNVYQLISPLYQ
                     RDFVMSMLLAFFILAVSFAVFIVDRREKW"
     gene            complement(8178..8477)
                     /locus_tag="PL056_07910"
     CDS             complement(8178..8477)
                     /locus_tag="PL056_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46978.1"
                     /translation="MKDLKMLKFSNISALLVLLVLSTALGFLERGNIQLVMILVTFLC
                     AGFFIKRAALYAGIAIMTIAVLLTLSSLVEVEKDRGGKFFKKFQFIIGNDKEESE"
BASE COUNT         2493 a         1861 c         1585 g         2646 t
ORIGIN      
        1 tttcgattat aaccgaattt tgagaatttc ttaaaaagtc gatagataaa tatggaatgc
       61 ccagattcct tcgttttctc tgggtcttat gttaaacccg acctgtgctg tgagtgctcc
      121 gatgggagag gcataagtca atccgccgcc ggcagtatat ctgaaaagcg aaccatctcc
      181 gatattgccc agcttctgcc agagatttcc catgtcaaaa aatgatataa gataaaaact
      241 ttcaatgagt ttgaacctga gttcgttcct catcacaaaa gaatatcttc cttcagccga
      301 tttttcattt tcaagatcat tgggctgaag ttcatcattg aaaaaacctc tgatagttgt
      361 gttgccgccg agtttaagta catcgtcaga agtaagatcg ccggaatgct ggataagaaa
      421 agcggtttca atgaaagcgt ggtaaataag tgtatcttta ggttcaagtt ctcctgcaaa
      481 gttcatcctg taacgcaaag gaatataaag ggaaagattg ttctcaaaat aggaaatgct
      541 tgtcgcttca ccgtaaaccg atgtcttgtt ggcatatttg aaaaaggtct tgtatccttt
      601 tgtcggaaag aaaagcgagt ccctgaaatc aatatagccc ctgaattcag gagaaatgac
      661 cagtctttca atgttgttat aataaacatc gccggcttca tccagttcgt gatctcgttc
      721 attctggtaa gcaatttcaa cacctgtaga aagaaaataa ttgttgctga accttctgaa
      781 aaaactgaag tacataccgt tcttgtcaat catgtatgga agccctgcat tacttctgat
      841 atcgtggata tggaacgcct cgacctgtgc agaaagggga aggaacggaa gataaagatc
      901 aggaaaaaga aatgcaagat tgaattttct ttcaaactgc tcaaaaagag tgaaatcact
      961 tgaataatac tttttgaact catctcccat gaaaagttct atctttcttg agatcttgaa
     1021 actgaacttt gttgaaaatc ccgaaccgcc gaaatttctc cattcagcgg aaccgaatat
     1081 tcttccacct tcatcactgc ttattccgat tcccggagag atcttaaatc tttcgacttc
     1141 acttactact accacaacat ccttttcttc cgacggaaat tcaggatcga taaagtttat
     1201 cattacagac tggtacatgc cggtctgaag aagatctctt cttctgtact ggaaagtgtc
     1261 ccccttgagc gtatctccgg gcctgatcct taacgcccct tttaccgccc attcctcggt
     1321 gatgaagttt ccggcaatta caacttcggt aactttgaca ggaaaaagat attccgcttt
     1381 gaacttaagc aagatcttct gcccgtcata ttcaagatca tgtgtaatat gtgcaaaaat
     1441 atatcctttt gaagaaagaa actcttttat ccttttgcga tatgcttcga cgatattttc
     1501 attaaacggg atcgccctgg gtagaaggat cttttttttc attatcctgt caagcaccgg
     1561 atcccccgtt gtagcggtaa cccctgtgag catgaacctt gtgttttcct tgactccgac
     1621 aagaagataa agtccctcgg aattatcaat gatttcggcg cttaacacag aaatatccat
     1681 atacccttca ttcagataga ccttttcgac gatctctttt atgtcttcaa gataggcctc
     1741 cggtatcccc agtggagaat cgggaagcat tactttgtcc tttttgcttc ttttcagcgc
     1801 cttgactctt tcgttgtctg tatctttata gccgcctccg gtctgggaac gggttatttt
     1861 tatctgcgga aaattctcct cttctatttt cttttcaatg aaatccttaa gacggttgac
     1921 aacaacattt ctgttaaaac ttgagccgcc ggtaaaaaca atgtcattaa tgaatcttct
     1981 ctctccttcg tcgatgttta ccgatatggt tctggtctga tcgttttttc tcagtgacag
     2041 atcacttata tctacatcga taacggcatc aggaaaaccg tatgcctgat aaaaaataag
     2101 aagttttctt tttatccctt cagcatcgaa atgatagttg ctcagccctg ttattattgt
     2161 tttcaaagta tccggtgaaa aagattcgtc ccctgttatg tcaagaatgt acaaaagacc
     2221 cttatttata gaaaaactta ctttcccgcc cgggagacgg tcggtattga cattaacatc
     2281 aagatagccc ctgtctttaa aatatgtatt gataaactca ttaatatcat tgatgttttt
     2341 gtcagtattg gggttgcctt taaatgatac tcccaattca tattcgagct tccttttttc
     2401 agatttgttc attgatccat agatccggat ctcattgatc ttttcctgaa ctccttcaat
     2461 tataactatc ttgagtgaaa ttgagttttc accggttttt tctgtcgcaa tgttgacaag
     2521 agtgttgaaa aaaccgttct ctctgtaaaa atagattatc tcgtccctta ttctctcaag
     2581 gtcgcttgta aaaaaatagg tgttctttcc tgttcctgaa gcagatttaa gctgtctgtc
     2641 ggaaaacttc gagttccctt ttatgcttat gtccgtaata atgagctggg gagaaccttt
     2701 caatataagt tttacgccgt ttttttcctt ttcaagcatt accgctatct gttcaaattt
     2761 ttttgtcgca tagagaagct tgaccgttct tctgatgttc tttattgaaa actcatctcc
     2821 ttctttaacc tttatcagcc ggctgaactt atccttttca agcggagtgt cccacactac
     2881 tgttgaaata atatcctgag cgattatttc ggctgaaaca aaaaacaaaa atatgaagag
     2941 taatgttaaa cgcttattca aaatagtact ccagccggaa atcaaaacct attgctccga
     3001 aactgctttc aagccggtcc ttgttcttcc atccgaggat cgtttcaaga ctttttgtct
     3061 tgtattttat ttcaattatc tgatcagtag tgtcggaagg gtttgactcg agagaaagaa
     3121 aaagatttct gtatatctcc ttttcggcga caagctttaa gacagtcttg tcatcaaatt
     3181 cagaatattt agggataagt tcgaactttg taagattgaa attctttctc acctgtgttg
     3241 ttatccccat aatgtcggtg acagccgcta tcgcaatgtc acctgagaac gagtccccaa
     3301 tgttccccca gagaagaaga gagacaagct gctgctgatc aagctgaggg atgctgtcaa
     3361 aattgacctc gagctctccg tcaaaagggt tgcctcttgc agtcatgatt attttgaaat
     3421 ctttttcatc atccacaact ttggcggtga catttctgaa tgattccaag tctataaaag
     3481 gatttattcc gttgccgtcc tcaaatgtta tgatgccccg ctgtattgta aaatcgttct
     3541 gcttgtactg gatctttccg tttttaagga gaatcgttcc tgacatctca ggtttccggg
     3601 cagttccagt gatagtcgta tcaatgaaaa ggtctgattc aataagattt gtctttatcc
     3661 tgatgttgtt cttaccggta actctgatgc tcagatctat cggcagggtt ttttcaattt
     3721 gggctcccgc cctttctctt ccaagctttg agatcatttt tataaaatca ctatcaagcg
     3781 aaagcggctg attgtaaacg atatttctta actcaatatc tccgctcact gatatctgtt
     3841 caggaccgaa catgattatg tcgaggtttg acgaaagacc gaaatctccc gtgagagcgt
     3901 attttcctgt caggtttgtc aacttgagct ttaaagcggc gttgtcaaac gggaacatgc
     3961 ttccttcacc actgaggaca actcttcccc ctcctgcaaa tccgtttaaa ttctcaattt
     4021 tccatttgtt cttgtcaaaa cttaacagcc cgttgaaacc gctgaaaatg atctggggat
     4081 ttttcagccg gtaagatccg tcaaccattc ttaattcgcc tttgatatcc tctttgtcaa
     4141 gagctacatc aaaatagaat tttccgtcaa cacctgttat ttcaccggga tagagtattt
     4201 caaccattct tgggaaaaga gtcccttttg ctgatatatg cggagaataa ttgacaattc
     4261 ttccttccag aaagaaattc gccgtttctc cgtcaaagta tgctttctcg atctcaagat
     4321 catccatctt gccggaaata ctgaaaggag cgttgtttct gacaaaaaat cctgactttt
     4381 ccgccatcag agttgtcact tttgcatcaa tattttcccc ttccattttt gcatttaaag
     4441 agagtttaac aaagaaatcc gacaccttgt gaacgaaatc cttgattttg agagaagcgg
     4501 acagattttc cggtttgaac tctttcatca tagcgctgaa aacaatgctt ttcccgagtt
     4561 ttccctttat gccaaaatcc atattctccc cggcaagttg agcagaaacc gcgagatctc
     4621 ccagcttaac accggaaaac gatatgccgt ttgcttcaaa ttttgctgaa acatcaggtt
     4681 ggtcaagaat tcccgcaagt ttgatatcaa gagacggctc ttcataatca aggtcggcag
     4741 gcagaggaaa atcatccttg aaaaatgatg aaaattttgc cgatatttca gtgtcaagat
     4801 ctttttttga cattctccct tcaatggcta ttgaactttt tccgtaattc agattcccaa
     4861 ttccgttaat aaaaacagga tctttgtcgt ttattttaaa aatgaaagaa tctgccgcac
     4921 gaactccttt gatcttcaga ttgtcagctt tgagcgaagc gttcagcttt gaaagaccgt
     4981 tcttataaac tccttctgca gtaagagcca cattgcctga aaaagcatcg gtctgaatat
     5041 caaaaacagc agcggcatcc tttaaagatc cttttgcatc gttaattaaa aaagagactg
     5101 gtaatatttc atcattaaaa tcaagtgaaa cgaaagaatc ctttgatgaa atagagttct
     5161 gcttaatgtc ttcaacacct atcctcagaa caaattcctt aagttttgtg tcaacggtac
     5221 agctctgagc attaaatccg tgaaaagagc atcctgtccc cgaaaaacgg ccggtaatgt
     5281 aaggattttc gtaaaaagag gtcaccagcg cctcgatcga acccctccct tttacattaa
     5341 agccggttat ttggttaaca tcttccagat ttatccagct gccggcaact accggaatgt
     5401 gaaatttcat gctgtctgca aaaccgaacc acgaatcctt cacgataagg cgactcgtct
     5461 catcctttgt tttcactata ccgtcgtgaa gcttaacacc atctgctccg accgtaacat
     5521 tgccggtcac aaaaacttcc cgcggaagat caagtatctc ctttttacct acatcgaact
     5581 cgtttacaac aagatcggtc ttgacatcag ccgacagcgt ttccgtagaa aaaacataat
     5641 cggcttttcc tgaaacgatg agatttacaa tgccgcttgt ggtcattctt ctcagacagt
     5701 catcaagatc aaatctgtaa agccgcacaa ctcccttgat ttccttggtt ttataaggaa
     5761 aaactccgtc aagcgaaaga aagaccttgt cgttatggaa caaattgagc cggtctatgt
     5821 gcattgtcga tggagttatc tgtaaaaatg catgaatatc ccatggtctg aacccttcat
     5881 aattaagacc tgacacagtt agagttgtat caatttttgg cactttccct gcaatatcaa
     5941 ggacatagtc agcataaacc tcgccttttg cagtttgtga aaagatttca actgcctcac
     6001 tcaaatctac ggcgaccctg cccgaaaggt ttcccgtttc tcctttcttt tctcccgaaa
     6061 tgaccatccc ttttgatgaa actgcaatat tatctatcgc ataaaaaaag tcttttgcct
     6121 caatgtttgc ataaaggtca acaatgaccg gctctttcag cgaagagtga aaatatctgt
     6181 tatcagccat ttcaatttca tattttccgt tgcccgaaat atctatgttc tttgcaataa
     6241 aaccattctg accgtctatc atcgccgaaa cactgagatt tttaataaaa acctcctcaa
     6301 gcggaaagaa cggaaaagtt tcaggaaatg tgatgtcgct cggctcaaga agccatttac
     6361 tgtcaaatgt cagttcgccg tcaatcagct caagatagtg aagcacatac tctttttgaa
     6421 gaaaacccga actttttctc cctgccgaaa aagtgagttt gcctgcttta aagctcagca
     6481 tgtcttttat ttcaatagag aggtcttcaa aaactatcct gcggcccgac tcgtaatcta
     6541 ttcttgagaa atcgtaacgg ataccgtaaa tatcaagaaa aaatgaagaa acgggataaa
     6601 atgcaaactt taccgtaaaa aggagggaaa cagtaaaaag aagcgttatt gataaaaatt
     6661 taattctgtt cacggcaacc ataaaaaatg gagcgggcga cggggttcga acccgcgaca
     6721 tccagcttgg gaagctgaca ctctaccaac tgagttacgc ccgcaaaagc cgtaggatat
     6781 atacctgcaa gatgccggat tgtcaagaat tccacttgta gagaccaaca aatcagctgc
     6841 ttccgaggtc acaaccgtta atgtatccgg ctgcgatttc ttttaaaagc ggttctctgc
     6901 ctgtgttttc ttttgttttc acaagttttt cactgaaaac cggaccgatt acgctcatgt
     6961 ttgctgaact ctgggttccc gggacgatcc tgtgttcaaa aagtatcttt gacacttcgt
     7021 cacaaagaac cacttttccg ttatgtataa gggcaagatg cagggcatac ttatcaaatt
     7081 catctatttc gtgggtgctt attatcagag tgcgcgactt taaagcttca ttgtgcagaa
     7141 ttgtgtgaaa tttcattctt accgccgggt caagatgctg cagcggttca tcaaatatca
     7201 tcacttccga atttgatgat atgacaagtg aattatgaag ccatgttttc atcccctttg
     7261 aatattgctc gagaggtttg tcagcgggta tctttaattc tttcatcatg ttaaagaatt
     7321 tttccctttc aaagccgggg tagaggagct cccacatgtc agcatattta tttgcatcaa
     7381 aattcttaaa aaaatctcta ttctcttcag caactgcaac agtaccgccg ttttttgttt
     7441 ttattattcc gctgctcggt ttgagctctt tgcagagtat ccttaaaagc gttgtctttc
     7501 ccgcaccgtt cggtccgaca aggataaaca ccgtacctgc gggaatatcg atcgtgacat
     7561 tgtccagtgc cgcttttttt ccaaaaaact ttgaaatacc ggtcgcggaa atcaccattt
     7621 ttccctcctg tcaacaatga atacagcgaa agaaacagcg agaatgaaaa atgcaaggag
     7681 cattgacatc acaaaatccc tctgatataa aggacttatt aattgataca catttgtttc
     7741 aggggtccct atattgccgg ctatcagatc tgttatcaaa agaaaaaccg gaaatgtgag
     7801 gttgtcagcc ccttttgtcg taacgagcat gagtatgccg aaatatgcac agtagaaaac
     7861 aaaaagacga gtaaaaacag caaagtcagt cttccctatg aaaaaagctt cccctatcac
     7921 cgttgctgaa aagatgatca gaaaaccagt tatgaaagcg aacagaaaga tctcactccg
     7981 ggaaaacgga agggaaagga tcatttttat tcttcttgaa ttgacatcag ccggcagaag
     8041 ctttgaaata agaataaaaa caaaaagata tttaatgtgt ccgggcagaa gaagagagag
     8101 aaacaaaaga ccgaatgtct tttttcttgt tgttgtttca agcagcagaa atgcctcaaa
     8161 atgatttaac agttttatca ttcactttcc tctttgtcgt ttccgatgat gaactggaac
     8221 tttttgaaga attttcctcc tctgtctttt tcaacttcca caagactgct taaagtgaga
     8281 agaacagcga tggtcattat tgcaatgcct gcataaagag cggctcgttt tatgaaaaaa
     8341 ccggcacaga gaaatgtcac gagtatcatg acaagctgaa tattccccct ttcaaggaac
     8401 cccagtgccg tagaaagcac cagaagcacc aacaaagcgg aaatattgga aaattttaac
     8461 atctttaaat ctttcatatt taaaaaatct ccgaaaaaac gggtgatatt accacgaata
     8521 aagaaaaagt tccagtttct gaaggtttaa gttatattca ttcagcactt gtgttgtttt
     8581 tcaaa
//
LOCUS       DAOOYB010000115         8580 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_9133_length_8580_cov_7.698299, whole genome shotgun sequence.
ACCESSION   DAOOYB010000115 DAOOYB010000000
VERSION     DAOOYB010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8580
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9133_length_8580_cov_7.698299"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..508)
                     /locus_tag="PL056_07915"
     CDS             complement(152..508)
                     /locus_tag="PL056_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HPM46979.1"
                     /translation="MKILIIFNREPYDSTDVTWNGLRLAGTLRKNGNDVRIFLMNDSV
                     DMARDVCKPPAGYDQDLSQMLKDLISNGVVVKVCGTCMARCGIHKNHPYFEGAEKSTM
                     ATLAEWVTESDKVLTF"
     gene            735..1712
                     /locus_tag="PL056_07920"
     CDS             735..1712
                     /locus_tag="PL056_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HPM46980.1"
                     /translation="MFLAILSIVAGLALLVWSADKFVEGSAAVARHFGMPSLLIGMVI
                     VGFGTSVPEMIVSALASLDGASGIALGNAYGSNISNIALILGVTALISPIAFHISVMK
                     IELPILTAVSLFAAFQLIDGEITRIDAILLIAVFTIYMIWAIRKALRHKKSKMAKEYE
                     KDIEQIKMPMKTAFFWLFAGLTVLIISSRMLVWGAVEIAHFFKVSDLIIGLTVVAVGT
                     SLPELASSVMAARKGEHDIALGNIIGSNLFNTLIVVGIAGLIKPLTVQPEVLNRDMVV
                     MILLTVSLFVLGFNFKNRKQGRINRFEGALLLFAYVGYTAYLIHFSFIK"
     gene            complement(1698..>2639)
                     /locus_tag="PL056_07925"
     CDS             complement(1698..>2639)
                     /locus_tag="PL056_07925"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HPM46981.1"
                     /translation="IEELDGTIGHIINYMKMEKAQIAYRKRILFPMSFIWFDGYKVFQ
                     TMHENKETAETSEWFIHNILKNYSDISYTRLYEAEGHYPSRYEFDRPLNRENIVDEWK
                     KGYGMIFWGGHGFPTTVARTVWLEDRNNNNLGENDEVESYQMVDPSDAKEIYSGQPGF
                     IVAISCLVGNVNSPGSITHRFLADGAAVGIISSTSVTDPSSTKWTDFSSEMDLSTVSE
                     DTAGVVFFEGLINGGYAGKIFYDYMSEFGQNPAGRVLDHKYMLNYFGDPTLTLYDTAV
                     DDIDDEEITDEEEHEDTENIPENDSENDSGCSILFYE"
     assembly_gap    2640..2739
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2740..3068)
                     /locus_tag="PL056_07930"
     CDS             complement(<2740..3068)
                     /locus_tag="PL056_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HPM46982.1"
                     /translation="MDSIFLKIMLPVLALLLFLRESYAEEISKGLIIATTENIRLNSE
                     KLDEFIKEKENRGFTVKIATEKDFGGEELKGYEKAQLIRSWLKENSSGYSFLLLIGDP
                     NPKMGDIP"
     gene            complement(3080..4597)
                     /locus_tag="PL056_07935"
     CDS             complement(3080..4597)
                     /locus_tag="PL056_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46983.1"
                     /translation="MKKSISLMITIFIFIFFLSCGGENTQKNDNESQNDEDSVVTENE
                     PDEDSVDDEAPLPTKPDDSFFSNPLNVQYTFKGYINEYEVINTDDEQKGSMELTLKMG
                     DLDLTLNDIPTSYTYTYPETAPENQRGKTVVAVISAGGVAQENGLQTYYIFINSFNAD
                     KLKELKKNNIREFSSADAEAGVNINKYYVKARADGSRVYRRCLIGMSPAENKSRFFIN
                     HSSNKDFAAEETMLAWGNVEVTTELAPEVTKNLTEYEGKFCSFSIDGEPLTKDEYEAE
                     MVKDVTQFDCEMPEGFMAPFSEKYGIMKIKGKINPPQSDTSTIGQANINLENKDIVLD
                     SLFQIQSGSDSVNEVLTLDSAGDINIISGNSHYKYKYFQAIVPKNFLKELKENGEESV
                     LIEDVLEHESVGSVLYSVEEKQVTSKTLQKVCIIATPSKTPASKMFLCFKNNTEFTDG
                     EEFQGAVNISLNNEESYLLEELEAETLSDLCYCASYSSTSYTPAADCEEFDKTDI"
     assembly_gap    4928..5027
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5133..5534
                     /locus_tag="PL056_07940"
     CDS             5133..5534
                     /locus_tag="PL056_07940"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HPM46984.1"
                     /translation="MNGFIQTGVRNVKGFTRWFDIPKGRTRFMVEKSWKKDLKAAIEN
                     GLLDIDMIYVFDALLHSEFSFDITVHDKPYPLFFHAAVRGNTKVVRFLVENGHVDVNQ
                     KDQRGWTAMDYAQKCRRYDVIHYLIEAGSIH"
     gene            5639..6574
                     /locus_tag="PL056_07945"
     CDS             5639..6574
                     /locus_tag="PL056_07945"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPM46985.1"
                     /translation="MTQPVEIKNVTKTFRHPMKVWDTIEAVKDFSFSVQTGEIVGFVG
                     HNGAGKTTTIKMLMGFIAPTSGEISIFGHKPGNREVRYKIGFLPERPYFYSELTAMDV
                     LKYFGKLSGMKGSNLIDRAEKVLRKVGIYNDRNRKLSGYSKGMLQRIGLAQALIHDPE
                     LVIMDEPMSGLDPIGRREVKEIIRTLRKDGKTVIFSSHILSDIENLSDRVLIIENGVK
                     KSFGTVREIIQPENIKYSIEFGCAAAMKDSAFSLFKGIKVVSDRFVIDCENIGDFNSK
                     LSAIVQKGYTIYDAKGVYPSLEEIVFKGSAEVGHE"
     gene            6567..7337
                     /locus_tag="PL056_07950"
     CDS             6567..7337
                     /locus_tag="PL056_07950"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPM46986.1"
                     /translation="MNRIFNVSYYTFLEMVRNKVLYILLFFAIFIFVLGIAITQMTIG
                     DELNIISDIGLGTIEIFSTVLSIFLGISLVQKELSLKTLHPLLSRPLSRAEFILGKFF
                     GMILLIVVEIAVMCFIFAALLFVYGGIDRFVGYMPAVYTIFLQTSTIIAVAVLCSTLT
                     EPAVAAMLTISFYLTGATSYNLVYAITSRTSETMAKIITALRYILPDFSHFNIKDNLV
                     YGRGLEGLSIGFASFYALFIVVIVLSLAIIFFNSKEIN"
     gene            7503..>8580
                     /locus_tag="PL056_07955"
     CDS             7503..>8580
                     /locus_tag="PL056_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014771987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM46987.1"
                     /translation="MWYNRFYQNNFENYLKNGKVLVIYGPRRVGKTSLVRKFTDSFNG
                     RIFAGAGEDAVLKEVINSGNIASMKAAFSGYEIVFIDEAQMIPDVGTALKIMVDHIPE
                     IKIIATGSSSFDLSGKLGEPLTGRMKTMLLFPVSMSEISKNIGGMEILQNLENYLIYG
                     TYPEVLTGVNNEEKQDYLIELKNAYLLKDILSLENIRNSDKLMDLLRLIAFQIGHEVS
                     LNELGNQLGMSKNSVEKYLDLLEKTFVIKKVRGFSRNLRKEIVKNSRYYFLDNGIRNA
                     VINNFSPLNLRNDEGMLWENFLFIERMKKQSYAGKHPNYYFWRTYDGKEIDMIEEEAG
                     VLNGFEFKWGNKKVKPPQLWKETYP"
BASE COUNT         2438 a         1586 c         1658 g         2698 t
ORIGIN      
        1 tttcaagcgg ctatgctgat gaccctgtca tggcagaccc tcagaaatac ggtttcaccg
       61 caagcatcac caaacctttc acaaaagcgg aactggtgag agttctgaat gggtatatga
      121 aagggtgagt aatactaata tttttaatga attagaaagt taaaacttta tcgctttcag
      181 tcacccattc ggcaagagtt gccatcgttg atttttcagc accttcaaaa tagggatggt
      241 tcttgtggat tccgcatcgt gccatacatg ttccgcaaac ttttacaacg actccgtttg
      301 aaataaggtc tttgagcatc tgtgaaaggt cctgatcgta tccggcaggc ggtttgcaga
      361 catctcttgc catatcaacc gaatcattca ttagaaagat cctcacatcg ttgccgttct
      421 tcctcagtgt tccggcaagc cgaagtccat tccatgtaac atcagtcgaa tcatagggct
      481 cgcggttaaa aatgataaga attttcatgt gttcctcctt ctttttgttg aggttgaata
      541 aactttaaaa acagtcaagt gcactttacg cttttgggtt aattcagtca agtgcacttt
      601 acgcttttag gcaaattcag tcaagtgcag tttgctgttt tttttatggt tcagtccatt
      661 ttcggttatc tttgacataa ttatcaaatg taataatatc gggtcgaaac tgttcttaaa
      721 tgatggagag ttctatgttt ttggcgatat tatcgattgt agcagggctt gcacttttgg
      781 tttggagtgc tgataaattt gttgagggtt ctgccgctgt ggcaaggcat ttcggaatgc
      841 catcattgct tatcgggatg gtcattgtag gattcggcac ttcggttccc gagatgatag
      901 tttcagctct tgcatctttg gacggtgcat caggaattgc tctgggcaat gcatatggtt
      961 caaatatctc taatattgca ctgattcttg gcgtaacagc tttaataagc ccgatcgcat
     1021 ttcacatctc tgtcatgaaa attgagcttc ctatcctgac cgcagtcagc ttgtttgcgg
     1081 cattccagct tattgacggt gaaataacca gaattgatgc gattctttta attgctgttt
     1141 ttacaatata tatgatatgg gcgatccgta aagcacttcg tcacaaaaaa agtaaaatgg
     1201 caaaagagta tgaaaaagat attgaacaga taaaaatgcc gatgaaaacc gctttcttct
     1261 ggctttttgc ggggcttacc gttctgataa tcagctcaag aatgcttgtc tggggggctg
     1321 ttgaaatagc tcatttcttc aaagtaagcg accttataat tggtcttacg gttgttgctg
     1381 tcggcacatc tttacctgaa cttgcatcgt ctgttatggc ggcaagaaaa ggcgagcatg
     1441 acatcgctct tggcaacata atcggatcta atctttttaa tacacttata gttgtaggta
     1501 ttgcaggact cataaaaccg ctgaccgttc agcccgaagt tctaaaccgg gacatggttg
     1561 taatgatact tctgaccgtt tctctttttg tgctgggatt caattttaag aacagaaaac
     1621 aaggtcgcat caacagattt gaaggggcgc tacttctttt tgcttatgta ggatacacag
     1681 cttatctcat acacttttca ttcataaaat aaaatagaac atcctgaatc attctcacta
     1741 tcattttcag gaatgttttc agtgtcttca tgttcttctt catcagtaat ttcttcgtca
     1801 tcaatatcat cgactgccgt gtcgtaaagg gtcagtgtcg ggtctccgaa atagttcagc
     1861 atatatttgt gatcgagcac ccggccggca ggattctgtc cgaactcaga catataatca
     1921 taaaagatct ttccagcata tccgccgttt atcagccctt caaagaaaac aacaccagct
     1981 gtatcttcgc tcactgtcga caagtccatt tctgaagaaa aatcggtcca ttttgttgaa
     2041 gaaggatcgg tgacacttgt tgaacttatt attccgactg ccgcaccatc tgcaaggaac
     2101 cggtgtgtga tcgatccggg agagtttaca tttccgacaa gacagcttat cgcgacaata
     2161 aatccaggtt ggccgctgta tatttctttt gcatcagaag gatcgaccat ttgataactt
     2221 tcaacttcat cgttttcacc gagattattg ttatttctgt cctcaagcca gacagttctt
     2281 gcaactgttg tcggaaaacc gtgaccgccc cagaagatca ttccgtaacc ctttttccat
     2341 tcatcaacaa tattttctct gttcagcggt ctatcaaatt catagcggct cggataatgc
     2401 ccttcagctt cataaagtct tgtgtagctt atatcactat aatttttcag aatattgtga
     2461 ataaaccact cgctggtttc tgcagtttcc ttgttttcgt gcattgtctg aaaaacttta
     2521 tatccgtcaa accagatgaa tgacatcggg aacagtattc tttttctgta ggcgatttgc
     2581 gctttttcca ttttcatata attaatgatg tgcccgattg ttccatcaag ttcctcgatn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng ggatatcacc catttttgga
     2761 ttgggatccc cgataagcag aagaaatgaa taacctgagc tgttttcctt caaccatgac
     2821 cgtatcagtt gagctttttc atatcctttc agctcttcgc cgccgaaatc tttttcagtc
     2881 gcaattttta cagtaaatcc acggttttct ttttctttaa tgaattcatc gagtttttct
     2941 gagttcaacc ggatattctc ggttgtcgct attatcaatc ccttagatat ttcctcagca
     3001 tatgattccc ttaaaaacag caggagggct agaacgggaa gcatgatctt aagaaaaata
     3061 ctatccatga caacctccat cagatatcag ttttgtcgaa ctcttcgcag tctgccgccg
     3121 gtgtatagct tgttgaactg tagctcgcgc agtaacaaag gtcggataat gtttctgcct
     3181 caagttcttc aagaagataa gactcctcgt tattgagtga aatgtttaca gcaccctgaa
     3241 attcttcacc gtctgtgaat tcagtattgt tcttgaaaca taaaaacatt tttgatgccg
     3301 gtgtttttga aggagtggct ataatacaga ctttctgcag agtttttgaa gttacctgct
     3361 tttcttcaac actgtaaagt accgatccta cgctttcgtg ttcaagaaca tcttcgatca
     3421 aaacactttc ttcaccattt tcctttaatt ctttaagaaa attcttaggg acaattgcct
     3481 gaaaatattt gtatttatag tgactgttgc ctgaaattat gttgatgtct cctgctgaat
     3541 ccagcgtaag aacttcattt actgaatcac tgcccgactg gatctggaaa agactgtcca
     3601 gaacaatatc tttgttttca agattaatgt ttgcctgacc aatcgttgaa gtgtcgcttt
     3661 gaggaggatt tattttccct ttgatcttca taattccgta cttttctgaa aacggagcca
     3721 tgaaaccttc cggcatttca cagtcgaact gtgtgacatc tttgaccatt tccgcttcat
     3781 attcatcttt tgttaatggt tccccgtcaa tagaaaaaga gcagaatttt ccttcatatt
     3841 cagtcagatt ctttgtcact tcgggggcaa gctctgttgt cacttcaaca tttccccatg
     3901 caagcattgt ctcttctgcg gcaaaatcct tgtttgagct gtgatttata aagaaccttg
     3961 acttgttttc tgccggtgac attccgataa ggcatcttct gtatactctt gaaccgtccg
     4021 ctcttgcttt gacataatat ttgttgatat taactcccgc ttcagcatcc gcagaactga
     4081 actctcttat attattcttt tttaattcct ttaacttatc agcattgaac gaattaataa
     4141 aaatataata tgtctgcaat ccgttctcct gtgcaactcc gcctgctgaa ataactgcga
     4201 caacagtctt tcccctttga ttttcgggtg cagtttcagg atatgtgtaa gtatatgatg
     4261 tcggaatatc attcaatgtc aggtcaaggt cgcccatttt caaggtaagc tccatcgaac
     4321 ctttctgctc atcatcagtg tttataacct catattcgtt aatatatccc ttgaaagtat
     4381 actgtacatt taaaggatta ctgaaaaaac tgtcatcagg ttttgtgggg agcggagctt
     4441 catcatcaac agaatcttca tcgggttcat tttcggtaac aacggaatct tcatcattct
     4501 gggactcatt gtcatttttc tgtgtgtttt cacctccgca gcttaaaaag aagataaaaa
     4561 taaaaatggt aatcatcaaa ctaatggact ttttcataac gacctccata aattaaaaaa
     4621 caaaccgaac aatcggtcag tattatgcaa cggctgtgcc atacttttat tatctaatga
     4681 aattaatagc ttacaatgat gtctttacaa tcattgtttt ttgcacataa tctataaagc
     4741 tgttttggag tataatggtg aataaaagaa caataaaagc gtcagataaa agaatgctca
     4801 aaggaataga aatgaaggag aaaattcttc attcagttct tgagatcgta ggactgaaag
     4861 gaattcagaa tctttcggca agtacaattt cagaaaaagt cggaatatca aaagccaata
     4921 tatttcannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaaa gattaaggga
     5041 tttcactaaa ctgacatgtc agtctaattt ttttgacaaa tactttttct gccgttatac
     5101 ttcacctgta aatttatctt tgggaggggt gaatgaacgg ttttattcag acaggcgtaa
     5161 gaaatgttaa gggctttaca agatggtttg acatacctaa agggaggaca aggttcatgg
     5221 ttgaaaaaag ctggaaaaaa gatttaaaag ctgcgattga gaatgggctt ctcgacattg
     5281 acatgatcta tgtttttgat gcacttctac acagtgaatt ttcctttgac ataacggttc
     5341 atgacaaacc gtatccgctt tttttccatg cggctgtgag aggaaatacg aaagttgtaa
     5401 ggtttcttgt tgaaaacgga catgttgatg tcaatcagaa agatcagcgt ggctggacgg
     5461 caatggatta tgcccaaaaa tgccgccgtt atgatgtaat tcactatctt attgaagccg
     5521 gatcaattca ttaagtttat tccagataac aaattaaagc tctaaatatt gacttattcc
     5581 actatgttct taaattaaat gttgtaaata gatctgaatt ttgaaagtgg aggaatcaat
     5641 gactcaacct gttgaaatta aaaatgtcac aaagacattc cgtcatccca tgaaagtatg
     5701 ggacactatc gaggcggtaa aagacttctc tttttctgtt cagactggtg agatcgtggg
     5761 atttgtcggt cacaacggtg ccggtaagac cacaacgatc aaaatgctga tgggctttat
     5821 tgcgcctact tcgggagaga tctcgatctt cgggcataag ccgggaaaca gagaggtgag
     5881 gtacaagatc ggttttcttc ccgaaaggcc ttatttttat tcagaactta cagcgatgga
     5941 tgtcttaaag tattttggaa aactgtcagg gatgaaaggt tccaatctga tcgacagggc
     6001 ggaaaaagtt ctaagaaaag taggcattta taatgaccgg aacagaaaat tgtcgggtta
     6061 ttcaaaggga atgcttcagc ggatcggtct ggcacaggca cttattcatg accccgaact
     6121 tgtaataatg gatgaaccga tgagcgggct tgacccgata ggaagaaggg aagtcaaaga
     6181 gataattaga acattgagaa aagacgggaa aacagtaata ttttcaagtc atatcttaag
     6241 tgatattgag aacttgtcag acagagtgct cattattgag aacggagtta aaaagagttt
     6301 cggaacagtc agagagataa tccagcccga aaacataaaa tattcaattg aattcggatg
     6361 tgctgccgca atgaaagatt cagcattctc actttttaaa gggataaaag tcgtttccga
     6421 caggtttgtg atcgactgtg aaaacattgg tgatttcaat tcaaaacttt ccgcaatagt
     6481 tcagaagggt tacacaattt acgatgcaaa gggggtttat ccatcactgg aagagatcgt
     6541 tttcaaaggt tctgcggagg tgggtcatga atagaatatt caatgtttca tattatactt
     6601 ttcttgagat ggtgagaaat aaagttctct atattcttct ctttttcgcc attttcattt
     6661 ttgttctcgg aattgccatc actcagatga caataggcga tgaacttaat attatcagtg
     6721 acataggact tggaacgatt gaaatattct caacagttct ttccattttc ctcggcattt
     6781 cactcgttca gaaagagttg tcgctcaaaa cccttcatcc gctcctttcc agaccgcttt
     6841 caagggcgga gttcattctt ggaaaatttt tcgggatgat acttctcatt gttgttgaaa
     6901 ttgcagttat gtgtttcatt tttgccgcac ttctttttgt ttacggcgga attgacagat
     6961 ttgtcggtta catgcctgcg gtttatacga tattccttca gacttcaaca ataatcgctg
     7021 ttgccgttct ctgttcaaca ctcactgaac cggcagttgc ggcaatgctc accatttctt
     7081 tttatctgac aggtgcaact tcatataatc tggtttatgc aatcaccagc agaacttctg
     7141 aaacaatggc gaagattatc acagcgctgc gttatattct tcctgatttc agccacttca
     7201 atataaaaga taaccttgtt tacggcagag gacttgaagg tcttagcatc gggtttgcat
     7261 cgttttatgc cctttttatt gttgtgatag tactttcttt agcgataatc ttctttaatt
     7321 ccaaagagat caactaaccg ttctctgtca gttttaagag tcccttactt ttatatggga
     7381 aatttaatca cacctctcaa aaaacttgcg gaatgactgt attcatataa aatttgtcag
     7441 tttgttgaca gaatttacca taatttggtc atttagtgtg ttttttacac aggagttgtt
     7501 gaatgtggta caatcggttt tatcagaata actttgaaaa ctatcttaaa aacggaaagg
     7561 ttcttgttat ttatggaccg agaagagtgg ggaaaacttc tctggtcaga aaatttactg
     7621 actcttttaa tggcaggatt tttgcaggtg caggagagga tgctgtttta aaagaagtga
     7681 ttaattcagg gaatattgct tcaatgaaag ctgctttttc aggatatgaa atcgttttta
     7741 ttgatgaagc tcagatgatt cctgatgttg gaaccgcatt gaagatcatg gttgaccaca
     7801 taccggaaat taaaataata gccacgggtt catcttcttt tgatctgtcc ggaaagcttg
     7861 gggaacctct tacaggaaga atgaaaacaa tgcttctgtt tcccgtttca atgtctgaaa
     7921 tcagcaaaaa tatcggtggc atggagattc tgcagaacct tgaaaattat cttatttatg
     7981 gaacatatcc cgaagtatta actggggtaa ataatgaaga aaagcaggac tatttaattg
     8041 agcttaagaa tgcatatctt ttaaaagaca ttctctctct tgaaaacata agaaattccg
     8101 ataaattgat ggatcttttg cggcttattg cttttcagat aggtcatgaa gtatcactga
     8161 atgaactggg caatcagctt ggcatgtcaa aaaacagtgt tgaaaaatat cttgatctgc
     8221 tggaaaaaac atttgtaata aagaaagtca gaggattttc aagaaactta agaaaagaaa
     8281 tcgtaaaaaa ttcaaggtat tactttttag ataacggcat tagaaatgca gtgataaaca
     8341 atttcagtcc tctgaattta agaaatgacg aagggatgct ctgggagaat tttcttttta
     8401 ttgaaagaat gaaaaaacag agctatgcag ggaaacatcc caattattat ttctggcgga
     8461 cttatgatgg aaaagaaatt gatatgattg aagaagaagc cggagtttta aacggatttg
     8521 aattcaagtg gggaaataaa aaagtgaaac cgcctcaact atggaaagaa acatatcccg
//
LOCUS       DAOOYB010000116         8563 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_9152_length_8563_cov_9.859426, whole genome shotgun sequence.
ACCESSION   DAOOYB010000116 DAOOYB010000000
VERSION     DAOOYB010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8563
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9152_length_8563_cov_9.859426"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..982)
                     /locus_tag="PL056_07960"
     CDS             complement(<1..982)
                     /locus_tag="PL056_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008775084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM46988.1"
                     /translation="MIERKIKEILKSKEGTNKALIVLGARQTGKTTLLKMLYGERDDV
                     VWLNGDEPDIQNLLENISSTRLKAILAGKKVVVIDEAQRIKDIGIKLKLITDNIPEIQ
                     VIASGSSSFDLANFINEPLTGRKWEFSLYPMSFSEMAAHNGFIEENRLLPHRMIYGYY
                     PEVVNSAGNEKEVLKQLSDSYLYKDIFTWENIKKPEKLMKLLQAIAFQVGSEVSANEL
                     SNMIGLDNQTVEKYIQLLEQTYIVFRLGSFSRNLRKELKRARKIYFYDNGIRNSIIAN
                     FAPVELRNDVGQLWENFLISERIKYLQNNMIFANRYFWRTVEQQEIDYIEE"
     gene            complement(1211..2533)
                     /locus_tag="PL056_07965"
     CDS             complement(1211..2533)
                     /locus_tag="PL056_07965"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM46989.1"
                     /translation="MDKSILFPIILEWQKIVAQTEGIFRSYQSELFELIGSKPIKIVT
                     GFRRSGKSFLVQKTISQLIAEKKIAKKNVLYLNFEDYQLASINNAQLLDEVFQLFMNE
                     IAEEGKKLIVLDEIQLVNDWDKFVRTIYEKYRNIEIILTGSNSELLSSELGSNLAGRF
                     VEIKILPFDFKEFTLIRGLNIKSEKDYFENINKINSLFGEYLNFGGLPELVSITTEKA
                     KQSYLEGVLSKVVLDDVVNRFNVRQPAVIDQIIKYLFIGTGNITSYARISTHLKTAGH
                     DVKSDTVANYVDFIVKTFAMYALEKLEYKQSRVFGTIKKFYSVDTGFSALYGGYIKIY
                     SRLLENVVFLKLKRESDQINFGQNESGKEIDFVVTERDRSVTNYQVAVTLNESNKDRE
                     LSSFVSIDRYISNGRHLFLSTDTDEKTIEYSGIEIERRNIVRWLLGVS"
     gene            complement(2609..3244)
                     /locus_tag="PL056_07970"
     CDS             complement(2609..3244)
                     /locus_tag="PL056_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46990.1"
                     /translation="MKNEMIKNRLKELSEFARKFKDPNDIQIFLNSIPYSTHKICQSP
                     YFVMKNRTAHCAEGAYFAAAVLEFMGFKPLVVDLSAENDDDHVIAVFKVDGLWGAVAK
                     SNTTVLRYREPVYRTIRELAMSYFEMYFNTLGDKSLTGYTLPINMNKFNKLNWLMTDE
                     NLDAPLGDALTVAKHFPLVPENIRKKLHKADPDLVKACFMTADPEGLFKPE"
     gene            complement(3247..4023)
                     /locus_tag="PL056_07975"
     CDS             complement(3247..4023)
                     /locus_tag="PL056_07975"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HPM46991.1"
                     /translation="MFLTLFVVSMLGGFMSGLLGLGGAVVLIPLMLTIPPILGVGDLS
                     MKTVSGLSMIQVLFSSISGMIIHKKNKFIHYESLLFIGTPMGFSAFAGSYLSKHVDNL
                     ILMYVFVILVFVAFVMLSMDKRFKKTDTPLAEIRINRAQSVLAGCVTGILSGMVGVGG
                     GFILIPFMTYILKIPFKATIGTSLGIIFIGAVTGSIGKIVSFQVDFALVLPVIIGSLL
                     AAQFGARVAKRTSPDTLRHILMAVVILSMIQVIYKIIEAG"
     gene            4169..5635
                     /locus_tag="PL056_07980"
     CDS             4169..5635
                     /locus_tag="PL056_07980"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HPM46992.1"
                     /translation="MPALKNVFKGTLLFLFVISTLQPLFAEKYEGLWNSFVEEGPHVT
                     DIQGGGGMDGVFDVEMSINSNQVYEPCIVWAHTPGDGIYQIQSTELYFSKRDATSGKW
                     STPAKITDNDFEEENVQFVVTQGTPEIFHVFYTASNPDNSNDLIMYTSSANGSTWSTP
                     VSIAEHFQIRYLTAKTSGDESFHLMYTPVETNYTTSVAYVWKAHYSGSQWSTPIVLAN
                     ESGVNYSKPLFSLETTNVDDDIIHAVWHESKGEYSDRQERVVFKIFSEEGNLWTDINP
                     GAVSDWETMSSFPSDILALGIPNEHYYVYVVKTAAAEGTSYWQPFLYTYSSYSDSWLA
                     PQKITESNSAQGESFLLIPSSTTTTTITIAWMEKSSGMKVRLADKGEFVSSAQSVPDW
                     SRPYEGLATGYLPVTGEYLKAANIGGDITFVWNVTSSWDSDKSPKLYTLQQWTEGEVK
                     SLEELNDTGDTGNTGDSGNTGNSGTDKKDDDSCSILYI"
     gene            5648..6091
                     /locus_tag="PL056_07985"
     CDS             5648..6091
                     /locus_tag="PL056_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018880485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HPM46993.1"
                     /translation="MKIFVDADACPLAILEILSRAVFRKKIYMVLVANQLKKGNFSEF
                     VSSIVVPGGADIADDKIVELMEEGDLVITADIPLASRVVEKKGFALNPRGEFYTEENI
                     RARLSMRNFMDELRGTGVDTGGPSTFNNKHKQQFSQALDKFLAKY"
     gene            6205..7317
                     /locus_tag="PL056_07990"
     CDS             6205..7317
                     /locus_tag="PL056_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007293360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM46994.1"
                     /translation="MFERLLASQVKKDLEKKMVFIGGARQVGKTTLAKSLIDKSGTYL
                     NWDITEHREMILKGEIPVSEMIVFDEIHKFKEWRQYIKGIFDRYGNDKKILVTGSARL
                     DYYRHGGDSLQGRYHYLRMFPLSAAELKINNYNDLIVLFNLGGFPEPFFSGSEIESRR
                     WSREYRTRLIQEDIVSLENTTELQKIELMMLRLPELVGSPLSINSLREDLNVSHKAVA
                     SWIEILERLYAVFRIPPVESNLLRAVKKEQKHYHYDWTLVKDEGLRFENMVAVHLLKW
                     VSWQADAFGREVELRYFRDIDKREVDFVVVENGKPVFCVECKLKDTSISKHLLYFINK
                     FPECNGWQITLDNPKNVTTKEGVRIAPASAFLSELV"
     gene            complement(7320..7940)
                     /locus_tag="PL056_07995"
     CDS             complement(7320..7940)
                     /locus_tag="PL056_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46995.1"
                     /translation="MRKVVVFIMIFVMSGMFLTLSADAAEPTFSADKERAFGFKIMGG
                     GRYDDLRMCVGSPAGVKGGPVGDIKLFMKFRMSLDWSMTVTIPVFRPILFGAGFKMLQ
                     YESDVAMEYKIKMSPKTDFVTGPGIGLSYHYGPDYNSERSGDKRTDSFFALGPMISYY
                     AAFDFKAPKHYATRLGINLFHVSLFRVDEFQYGKVFGGALEFGMYF"
     gene            complement(7958..>8563)
                     /locus_tag="PL056_08000"
     CDS             complement(7958..>8563)
                     /locus_tag="PL056_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46996.1"
                     /translation="DDIAECGNGIVEPGEECEPSEGTCCTSECKLVVADTVCREKNGE
                     CDVAEICNGTDNYCPEDKYLEDGSFCIGDGEPWTVYSCESGICVGETVIPDLDTDDSD
                     DTDDDLSDEVLTESDDTDDYDDMSDFDDMNDTDDSDVNDDLDIIDDSDAGNTGDTGDT
                     GNTGNSGNTAEDEDLFDESPVNDEDGVYIISGEGCSCSFII"
BASE COUNT         2621 a         1785 c         1601 g         2556 t
ORIGIN      
        1 tttcttcgat ataatcaatt tcctgctgtt caactgtccg ccagaaatat cggtttgcaa
       61 agatcatatt gttctgaaga tattttattc tttcgctgat caggaaattc tcccataact
      121 gtccgacatc attcctgagt tcaactggtg caaaatttgc gataattgag tttctaatac
      181 cgttatcata aaaatatatt ttccgtgccc gcttaagctc ctttctcaag tttctgctga
      241 aagatccaag ccggaaaaca atataagtct gctcaaggag ctggatatat ttttcaacag
      301 tctgattgtc aagtccgatc atattcgaca attcattggc agaaacttca ctgccgacct
      361 gaaaagcgat cgcctgaaga agtttcatca gtttttccgg ttttttaatg ttttcccatg
      421 taaaaatgtc tttataaaga tagctatctg acagttgttt caatacttct ttttcgtttc
      481 ccgcagagtt gacaacttcc ggataataac catagatcat gcgatgcgga agcaatctgt
      541 tctcttcaat aaaaccgtta tgtgcagcca tttctgaaaa ggacatcgga tataaagaga
      601 attcccattt tctacctgtc agcggctcgt ttatgaaatt cgcaagatca aaagaggaag
      661 aaccacttgc aataacttgt atttcaggaa tattgtcagt tatgagttta agttttatac
      721 cgatatcctt tattctctgt gcttcatcta tcaccacaac tttttttcca gctaaaatcg
      781 ctttcaaccg ggtggaactg atgttttcca gtaaattctg aatatcgggt tcatcaccgt
      841 taagccagac aacatcatcc ctttctccat aaagcatttt gagcaatgtc gttttgcctg
      901 tctgccttgc accaagaacg atcagtgcct tgttcgtccc ttctttgctt ttaagaattt
      961 cttttatttt tctctcgatc attccaactc ctgcgaacct attcgcaaaa tatacgaata
     1021 ttccaactat cacagtcgca aaatatgggt tttctggcta aaaatcaagt tttctgcgaa
     1081 tttgacggac caggaacaag aaaagttata gttaagataa tgggttaggt ttatttttta
     1141 agattttatc aactcttcaa ttttttcctt ccaattcgca ccctttttaa aaagaattgt
     1201 gtctgccatt ttatgatact cccaaaagcc atctcacaat attacgcctt tcgatctcta
     1261 tgccggaata ttcaattgtt ttttcatcag tgtctgttga aagaaacaga tgccttccgt
     1321 ttgaaatata cctgtcaatt gaaacaaaag agcttaactc tctatcttta ttactttcat
     1381 ttaaagtcac tgcgacttga tagttcgtga ccgagcggtc tctttctgtc accacaaaat
     1441 ctatctcttt gccggattcg ttctgaccga aattaatctg atccgactct cttttaagct
     1501 tcaaaaacac aacattttca agaagtcttg aatatatctt aatataaccg ccgtaaagag
     1561 cggaaaaacc agtatcaact gaataaaact ttttaatcgt gccgaaaaca cggctctgtt
     1621 tatattccag tttttcaagt gcatacatgg caaaagtctt cacgatgaaa tccacataat
     1681 ttgcaacagt atcagatttt acatcatgtc cggcagtttt gagatgtgtt gaaatccttg
     1741 cataactggt aatatttcca gttccgatga aaagatattt tattatctga tcaatgacag
     1801 caggctgcct gacattaaaa cgattgacaa catcatcaag aacaactttg ctgagtactc
     1861 cttcaagata actctgcttt gctttttcag tcgttattga aacaagttca ggtaagccac
     1921 caaaattcag atattcccca aacaatgagt tgatcttatt aatattttca aaataatctt
     1981 tttcgctttt tatatttaat cctctgatca aagtgaactc tttaaaatca aatggcagaa
     2041 tttttatctc cacaaacctt ccggcaagat ttgaaccgag ttccgatgac agcagttcag
     2101 aattggaacc ggtaagaatt atttcgatat tgcgatattt ctcataaatc gtccgtacaa
     2161 atttatccca gtcattaaca agctgaattt catcaagaac tataagtttt tttccttctt
     2221 cggcaatctc attcataaaa agctgaaata cttcatcaag caattgagcg ttatttattg
     2281 acgcaagctg ataatcttca aaattaagat acagcacatt cttttttgct atttttttct
     2341 ctgcaattaa ttgagaaatc gttttctgaa ccaggaatga cttgccgctt cgtctgaatc
     2401 cggtaacgat cttaatgggt tttgaaccaa tcagctcaaa cagttctgat tgatatgaac
     2461 ggaatatccc ttcagtttga gcaactatct tctgccactc caaaataatc gggaaaagaa
     2521 ttgatttgtc catttttgca cctcataaag atggttttcg gtccatttat acaccctgta
     2581 aggtggaaag tcaaggattt atgctttttt attccggttt gaacaatcct tccggatctg
     2641 ctgtcatgaa acacgctttg accagatcag gatctgcttt atgaagtttt tttcttatat
     2701 tttccggaac aagtgggaag tgcttggcga cagtgagagc atcaccaagt ggtgcatcaa
     2761 ggttttcgtc agtcatcaac caattgagtt tattgaattt attcatattt attggaagtg
     2821 tataaccggt gagtgacttg tctccgagtg tgttgaaata catctcaaaa tagctcatcg
     2881 caagttcgcg aattgtgcgg taaaccggct cacgatatct caaaacagtc gtattcgatt
     2941 ttgcaaccgc cccccaaagc ccgtcaactt tgaaaaccgc gatcacatgg tcgtcgtcat
     3001 tttctgcact gagatcaact acaagcggct taaaacccat gaattcaagc actgccgcgg
     3061 caaaatatgc cccttctgca cagtgagcag ttctattttt cataacaaaa taaggggatt
     3121 gacatatctt atgagttgaa tatggaatgg agtttagaaa tatttgtata tcattaggat
     3181 ctttaaactt tcttgcaaat tcagacaatt cttttaaacg gtttttgatc atttcgtttt
     3241 tcataactac cctgcttcaa ttattttata tatcacctgg atcatgctta aaatgacaac
     3301 agccatgaga atgtgcctga gcgtgtcggg agaagttctt ttggcaaccc ttgctccgaa
     3361 ctgtgcggca agaagtgatc cgataatgac aggaagaacc agtgcaaaat ccacctgaaa
     3421 tgaaacgatc ttgccaattg agccggtaac tgccccgata aaaattattc caagacttgt
     3481 tccgatcgtc gctttgaaag ggattttaag aatatatgtc atgaacggaa tcagaataaa
     3541 tccaccgcct acacctacca ttccggacaa tattcctgtg acacaaccgg ctaaaacaga
     3601 ttgagccctg tttatcctta tttcagccag aggagtgtct gttttcttga atcttttatc
     3661 cattgaaagc ataacaaatg caacaaacac aagaattaca aatacataca taaggatcag
     3721 attgtcaaca tgttttgaaa gataggaacc ggcaaacgct gaaaatccca tcggagttcc
     3781 tataaacagc agtgattcat agtgaatgaa tttgttcttt ttatggatta tcatgcctga
     3841 aattgaagaa aaaaggactt gtatcatcga taatcccgac actgttttca ttgaaagatc
     3901 ccctaccccg agaattggag gaatggtcag catcagtggg atcagaacga cagctccgcc
     3961 aagaccgagc aaccccgaca taaaaccgcc gagcatgctt acaacaaata aagtcaggaa
     4021 caaacttctg cctcctcaat taaaaaccgg aacagttcaa tataatctta aatcgctgct
     4081 gaatcaatga aacaaaagaa gttttgttga taaatcatcg tctaaactgt attattacaa
     4141 gatgttcatt aatttatgga gaatcgccat gccagcattg aaaaatgttt ttaaaggaac
     4201 tcttttgttt ctatttgtta tttcaacttt gcaaccgctt tttgcagaaa aatatgaagg
     4261 gttgtggaac tcttttgttg aagaaggtcc tcatgtaact gacattcagg gcggaggcgg
     4321 aatggacggt gtttttgatg ttgaaatgtc aataaacagc aaccaggtct atgaaccgtg
     4381 cattgtctgg gcacacactc cgggagacgg aatttatcag atacagtcaa cagagctcta
     4441 tttttcaaaa agagatgcaa catcaggcaa atggtcaaca ccggcaaaga taacagacaa
     4501 cgattttgaa gaggaaaatg tccagttcgt tgtcactcag ggaactcctg agatatttca
     4561 tgttttctat accgcgtcaa accctgataa cagcaatgat ttgataatgt acacctcttc
     4621 agcaaacgga agtacctggt caacaccggt aagcattgcc gaacattttc agattcgtta
     4681 tttaactgca aaaacttcag gagacgagtc attccacctt atgtacacac cggttgaaac
     4741 aaattatacc acaagcgtcg catatgtctg gaaagcacat tattccggct cgcagtggtc
     4801 aacacccatc gtgcttgcca acgaaagcgg cgtaaactat tccaaacccc tcttttcgct
     4861 ggaaacaacc aatgttgatg acgatataat tcacgcagta tggcatgaat caaaaggaga
     4921 atattcagat agacaggaaa gagttgtttt taaaatattc tctgaagaag gaaatctgtg
     4981 gactgacatt aaccccgggg ctgtttccga ctgggaaaca atgtcgtctt ttcccagtga
     5041 tattttagcg ttaggtatcc ctaacgagca ttattatgta tatgtcgtta aaactgcggc
     5101 agcagaagga acaagctatt ggcagccgtt cctttataca tacagctcat acagcgactc
     5161 ctggcttgca cctcaaaaga taacggaatc aaattcagca caaggggaat cctttctgct
     5221 gatcccttca agcacaacta caactacaat tactatcgca tggatggaaa aaagctcagg
     5281 aatgaaagtc cgtcttgccg ataaaggtga atttgtaagt tccgctcaat ctgttcctga
     5341 ctggtcaaga ccttatgaag gtcttgcaac cgggtatctc cctgtaactg gtgaatatct
     5401 caaagcagca aacatcggag gagacataac atttgtctgg aatgtgacat caagctggga
     5461 ttcagataaa tcaccgaaac tctacactct tcagcaatgg acagaaggtg aagtgaaatc
     5521 gcttgaggaa cttaatgaca caggtgacac cggaaacaca ggagatagcg gaaataccgg
     5581 aaattccgga actgacaaaa aagacgacga cagttgttcc atcctttata tctgatgata
     5641 atttaaaatg aaaatattcg tcgatgcaga tgcctgtcct ttggcaatac ttgaaatatt
     5701 gtcaagggcg gtttttagaa aaaagatcta tatggtgctt gtggcgaatc agcttaaaaa
     5761 agggaacttt tcagagtttg taagttcaat tgtggttccg ggaggagctg atattgctga
     5821 tgacaaaata gttgaactta tggaggaagg tgatctcgtc attaccgctg acatccctct
     5881 tgcttcaaga gttgttgaaa aaaaaggttt tgcactcaat ccaagaggtg aattttatac
     5941 cgaggaaaat atcagagctc ggctttcaat gagaaatttt atggatgaac ttcgcggaac
     6001 aggcgttgac accggtggac catcaacatt caacaacaag cacaaacagc agttttctca
     6061 ggcacttgac aagtttctgg caaaatatta aacaaccaat cacggatttt tttgcccaca
     6121 gtagaaaaaa ctcgcgagtt tttttgcttt gagtagaaaa aactcgtata atgaaccaac
     6181 ttgctggttt ttttgaggtg ttttatgttt gaaagattgc ttgcctccca ggttaaaaaa
     6241 gatcttgaaa aaaaaatggt ttttatcgga ggggcgcgtc aggtgggaaa aaccactctt
     6301 gcaaaatcgc tgattgataa atcgggtact tatcttaact gggatattac tgaacacagg
     6361 gaaatgatac tgaagggtga gatacctgta tctgaaatga tcgtttttga tgaaattcat
     6421 aaatttaaag aatggcggca gtatattaaa gggatattcg accgttacgg aaatgataaa
     6481 aagatccttg tgacaggaag tgcccggctt gattattaca gacatggagg tgattcactt
     6541 cagggcagat atcactatct tcgaatgttt ccgctttcgg ctgccgagtt aaaaataaat
     6601 aattacaatg acttgatagt tctttttaat cttggcggct tcccggaacc ttttttttca
     6661 gggtcagaga tagaatcaag aagatggtcg agagaatata ggacaagact cattcaggaa
     6721 gatattgtct ctttggaaaa cacaaccgaa ttgcagaaaa tagagttgat gatgctccgg
     6781 cttccggaac ttgtcgggtc gccgctttca ataaattctc tgcgcgaaga tctcaatgtc
     6841 agtcacaaag ctgttgcaag ctggattgaa atacttgagc ggctttatgc agtttttcgt
     6901 attccgccag ttgaaagcaa tcttctcaga gctgtgaaaa aagaacagaa acactatcac
     6961 tatgactgga ctcttgtaaa agatgaagga ctccgttttg aaaatatggt tgctgtacat
     7021 ctcctgaaat gggtaagctg gcaggcagat gcgtttggtc gtgaagttga actccgctat
     7081 ttcagggaca ttgataaaag agaagttgat tttgtcgttg ttgaaaacgg caaacccgtt
     7141 ttttgtgttg aatgcaagct taaagacact tccatttcaa agcatctgct ttatttcatt
     7201 aacaaatttc ctgaatgcaa cggctggcag ataacactcg ataatccaaa aaatgttaca
     7261 acaaaagaag gtgtcagaat tgctccggca tcagcattct taagtgaact ggtttaattt
     7321 cagaaataca tcccgaattc aagtgcgccg ccgaagacct ttccgtactg gaactcatcg
     7381 actctgaaaa gtgagacatg gaaaagattg atgccgagcc gtgttgcgta atgtttcggg
     7441 gctttgaaat caaaagcggc ataatatgag atcatcggtc cgagtgcaaa gaagctgtca
     7501 gttcttttgt cacctgaacg ctcagaattg tagtcaggtc cgtaatgata gctgagtccg
     7561 attccggggc ctgttacaaa atcagttttt ggtgacattt tgatcttgta ttccattgcc
     7621 acatctgatt cgtactgaag cattttaaag ccagctccga aaagtatggg tcgaaaaact
     7681 ggaatagtaa ctgtcattga ccagtcaagt gacattctga acttcatgaa aagttttata
     7741 tctccgaccg gacccccttt cactccggca ggacttccga cacacattct aaggtcatca
     7801 tatcttccgc cacccatgat cttaaacccg aatgctcttt ccttatctgc tgaaaatgtc
     7861 ggctcagcgg catcagcaga aagtgtgaga aacatcccgc tcatcacaaa tatcataata
     7921 aaaacaacta ctttcctcat ggctcacctc atatatctca aataataaac gaacagctgc
     7981 aaccttcacc ggatataata taaacaccgt cttcgtcatt aacaggggac tcatcaaata
     8041 aatcttcatc ttcagcagta tttccagagt ttcctgtgtt gcctgtgtca cccgtgtcgc
     8101 cagtatttcc agcatcagaa tcatcaatta tgtcaagatc atcattaaca tctgagtcat
     8161 cagtatcatt catatcatca aaatcactca tatcgtcata gtcatcagta tcatccgact
     8221 ccgttaaaac ttcgtcggac aggtcatcat ctgtatcatc tgaatcatct gtgtcaagat
     8281 cagggatcac agtttcaccc acacaaattc ctgattcaca tgaataaact gtccagggtt
     8341 ctccatctcc aatacagaat gaaccgtctt ctaaatattt atcttcagga caatagttgt
     8401 ctgtaccgtt acagatctca gcaacatcac actcaccgtt cttttccctg cagactgtat
     8461 cagctacaac aagtttgcat tctgatgtac agcatgtccc ttcagacggc tcacattcct
     8521 cacccggctc aacaatgccg ttgccgcact cagcaatatc atc
//
LOCUS       DAOOYB010000117         8498 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_9243_length_8498_cov_12.277390, whole genome shotgun sequence.
ACCESSION   DAOOYB010000117 DAOOYB010000000
VERSION     DAOOYB010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8498
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9243_length_8498_cov_12.277390"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            27..1871
                     /gene="dxs"
                     /locus_tag="PL056_08005"
     CDS             27..1871
                     /gene="dxs"
                     /locus_tag="PL056_08005"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HPM46997.1"
                     /translation="MIEIIKRSDIAELEKIAQNTREIMIDAASKNGGHLASSLGAVEL
                     TIALLKVFDIPDDKIIWDVGHQSYAYKILTDRSEKFNTLRTLGGISGFPKRCESPYDF
                     FGTGHGGTSISAGLGLREALKIRNINSKVISVIGDGAMTSGLALEAMNNVDETGKNTI
                     TVLNDNDMFISTSVGSLSRWFSRALSGQKYTSARSEIKQIIAKLPPFFHGEKIIELIR
                     KTINSSKSLLTPGILFEGFGYQYVGPIDGHNIEELIETLEDIKPNSEPVLLHVHSVKG
                     KGYEPAERNPRKFHGVGPFDKKTGETKPSLPSFTSYLSDYLPKLFKRHGNLVAITAAM
                     PDGTGLNTLQNIYPHRVYDVGMSEGHAVTFAAGLAAGGSRPIVAIYSTFLQRAYDSIV
                     HDVALQNLPVIFLIDRAGLVGDDGATHHGLFDMSYLRIIPNLTLMAPRDEHEMARMIE
                     FAVNNVSGPVAIRYPRGSGSGKRHYNRLTPLKSVKGELLATGKDPVMIISVGIMANMA
                     SEAVGKLEKKGHKVFHYDLRFIKPLPEELFEIINKNKINKIVTIEDGIMAGGAGSAIL
                     EALSNRNLDVKCRIIGINDTFSTHGTQNELKKLEGLTVENIVKSVLDK"
     gene            1872..2942
                     /gene="ispG"
                     /locus_tag="PL056_08010"
                     /gene_synonym="gcpE"
     CDS             1872..2942
                     /gene="ispG"
                     /locus_tag="PL056_08010"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF001540.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HPM46998.1"
                     /translation="MKKSRVITLGNILIGGDNPPVIQTMITTPMNNFDKALAEAETAF
                     ELGCRVVRTAFKNGDEEQSLKDMVKSFKGEIIADIHFDYRLALMAIDAGVSGVRFNPG
                     NVGGKERVKEIIESAKKRPEMAIRIGVNGGSLEKEILNKYNGVTWEGLVESSLFWADY
                     IEQELGYRNFKISAKSSSVPETIAACRMIREKCDAPLHVGITEAGGGKRGIIKSTAGL
                     SILISEGLADTFRVSLTASIVEEIQTANTILKSLGLLKTGADIISCPTCGRTHGAVIE
                     YYNKLEKWFNEQIWWMKPALKVAVMGCEVNGPGEAREADLGIALGKNCALYFEKGKII
                     NKFNDQDEAFEHLLKRISENWN"
     gene            2959..>3243
                     /locus_tag="PL056_08015"
     CDS             2959..>3243
                     /locus_tag="PL056_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM46999.1"
                     /translation="MKKTILVLIIAGFLSFSCSNGSSDENPDLEQNDSSAINDEDSSD
                     TGDTGNSGDSGNSGNSGDTGDTGNSGNSGDSGNSGDSGNSGNTGDTGNSGN"
     assembly_gap    3244..3343
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3344..3604
                     /locus_tag="PL056_08020"
     CDS             <3344..3604
                     /locus_tag="PL056_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47000.1"
                     /translation="EKAIYVSGLSYMFPNQENDQDIFIAAVDFQGEVLWHHFIGTDED
                     DWSYGLDAADGKLFVVGITQGIIEGDGSAISNEDGAILFTEE"
     gene            complement(3612..5372)
                     /locus_tag="PL056_08025"
     CDS             complement(3612..5372)
                     /locus_tag="PL056_08025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HPM47001.1"
                     /translation="MHSKVIKVIFLTVLITVSMQLSGVVKKTIMIPMSDGTGLETDIY
                     FPDNYQTESYPVILNRSPYDRTQKEESGGNMADSNVVFIAQSFRGTFGSEGTHGVFHT
                     EGWGEHMDGYDTLKWITDQPWCNGKIATIGGSAEGLVQYFMAGLDEKPGLIYQIITVA
                     SGDLYNGTFFPGGILREHAITTWLNDQKASFFIDDMMTGDMYSKSNDYWDVTDIFKKK
                     ENLNIPAAHLAGWFDMFGKYQIDTFRLFTEAGATDQHLVIGPWDHNKFMTNISGELTF
                     PENAAKHYEGEDDPVFAYLIHYFWPSYTQKPEMATVTYYTIGDVKKENAPGNEWKTAD
                     KFPPDDASELSLSAGADLVLKAGDCGEGEFILKFDLNDPFPSLCGNNLTINPGPCDVS
                     EYRERTDVMSFITEPFLEPTEITGAIGFHAVFAADVEDFDMVVIFTDIYPDGTEYLML
                     EGAQKVRFRNGLENESLIEKGALTEMEFNAGYISIIFDKGHRLGIHVAPAYYPKYRIP
                     STVADYWNDEKAKGEIKFDLSKTKVTVDIIGEWGGEKCEATEEEPDADSISEADELND
                     EESDESIEKKSSSGCIITVF"
     gene            complement(5470..6819)
                     /locus_tag="PL056_08030"
     CDS             complement(5470..6819)
                     /locus_tag="PL056_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47002.1"
                     /translation="MRRFMFFAMIFAGFMLVSCGNEVNTVSDNEGTNDESVNDDETSD
                     EINDETTDEISDETVDETVDEITDETVDESQDEQNDEDTDSGECKNLNVKYDSTGLEL
                     IAKDISLAVHSNGWGDLLETLTMETEGSKLLEVPEENPYDGGTPSYFVYETAEGFFTE
                     TDYAVFGDTINVDLDPVMPEHLNGAIFMIQYYFGPYTLYDADVKVYKDNSLVGCFKTD
                     GNGRFVIDLPDGDYTFAFKDQDGSYYDEEITEYNENVTVNSNYVELRVPAEAQMEKPN
                     IYLYPEETVYLDITLDFPQGGHVTTSIPEYGTGWNVRVESDGTIDGKYGYLFYESQNP
                     DVFQYRNGWTVENGDLEEFFRINLSAYGFAGREIEDFIEWWIPRLDGGCYNIYPQTAA
                     EIDPVVKLNISIEPDSVQRLSYAVKETSTCVAHLEEPVIEPFVREGFSVLEWGVILR"
     gene            complement(6937..7245)
                     /locus_tag="PL056_08035"
     CDS             complement(6937..7245)
                     /locus_tag="PL056_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HPM47003.1"
                     /translation="MTVGNFEFKGTGLGFLWLLIWTTVVTAITFGLFLPWAVSAQMRW
                     IAENTFVTGRKLVFKGTGLGFFGTYILILILTVVTLGLYAPWGCCQFIRWMTNNMDFA
                     "
     gene            complement(7383..8432)
                     /gene="corA"
                     /locus_tag="PL056_08040"
     CDS             complement(7383..8432)
                     /gene="corA"
                     /locus_tag="PL056_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013898671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HPM47004.1"
                     /translation="MKISKKKGMSPGSLVFIGEKKREKMDINIMDFSADHFTEKSVAD
                     INECAEFRDSKNVSWLDFLGLHDVEKIDEIGKIFDVHPLVLEDILNTNQRPKIEIFDD
                     HFFIVFKMLFFDGKSSVVVSEQVSLIVRKNKVLSFQEKESEVFASLRERIRKSKGRTR
                     SSGADYLAYEIFDVIVDNYLEILEKIGEEIEVLEEELMSNPASNTLGKIYRLKRDIFL
                     VRKSVWPLRDVVSRLERGEIDVIEQKTEIYLRDLYDHIVQVIDTVETFRDISTGLLDL
                     YLSSVSFKMNDVMKVLTIISTIFIPLTFIAGVYGMNFENMPELKWSYGYFISLGLMSV
                     IFVSMVIFFKKKKWW"
BASE COUNT         2578 a         1809 c         1624 g         2387 t
ORIGIN      
        1 gctgaatatg ttttgaggag aaagaaatga tcgaaataat aaaaagatct gacattgccg
       61 aacttgagaa aatcgctcag aacaccagag agataatgat cgatgccgct tcaaagaacg
      121 gtggtcatct cgcttcgtct ctgggtgctg tcgaacttac gatcgccctt ttgaaagttt
      181 ttgacattcc ggatgacaag ataatctggg atgtgggtca ccagtcatat gcctacaaaa
      241 tactgacaga cagatcggaa aagttcaaca ctttaaggac attaggtgga atttccggtt
      301 tccctaaaag atgtgaatcc ccttacgatt ttttcggaac aggtcatgga ggaacttcaa
      361 tttcagccgg actcgggtta agggaagctc tgaagatcag gaacatcaat tcaaaggtca
      421 tttcggtgat cggagacggc gcaatgacaa gcggtcttgc actggaagcc atgaataatg
      481 ttgatgaaac cggaaagaac accatcactg ttcttaatga caatgatatg ttcatttcaa
      541 cttcggtcgg gtcactttca aggtggttca gtcgcgcact ttcaggccag aaatatactt
      601 ccgcaaggtc tgaaattaag cagataatcg caaaacttcc accgttcttt cacggcgaaa
      661 agattattga actcatcaga aaaacgataa attcgtcaaa atcgctccta actcccggaa
      721 ttctttttga aggtttcgga tatcagtatg tcggtccgat cgacgggcac aacattgaag
      781 aactcattga aactcttgaa gatatcaagc caaacagtga accggtgctt cttcatgtcc
      841 actcagtaaa aggaaaagga tatgaaccgg cagagagaaa tccaagaaaa tttcacggag
      901 ttggcccttt tgacaaaaaa accggtgaaa caaaaccatc tctaccctct tttacctcat
      961 atttaagcga ttatcttccg aaacttttta aacggcacgg aaatcttgtc gcaataactg
     1021 ccgcaatgcc tgacggaacg gggcttaaca ctcttcagaa catttaccct cacagggttt
     1081 atgatgtcgg aatgagcgaa ggacatgcag taacatttgc cgcagggctt gccgcaggcg
     1141 gttcaagacc gatcgttgcg atatattcca cttttcttca gcgggcttac gacagcatag
     1201 ttcatgatgt ggcgctccag aaccttcctg ttatttttct tatcgacaga gcagggcttg
     1261 tcggcgatga cggagcgacc catcacggac tttttgatat gagttactta agaataatcc
     1321 cgaatcttac tttgatggca ccccgggatg aacacgaaat ggcaagaatg atcgaatttg
     1381 cagtgaacaa tgtttcaggg cctgtagcaa taagatatcc gcgaggaagc ggctctggca
     1441 aaaggcatta caaccgtctc actcccctta aatcggtcaa aggtgaactc cttgcaacag
     1501 gtaaagaccc tgtaatgata atatctgtcg gaattatggc taacatggca agtgaagcag
     1561 tcggaaaact tgagaaaaaa ggacataaag ttttccatta cgacctccgc ttcatcaaac
     1621 cactccctga agaactcttt gaaatcatta ataaaaacaa aatcaataaa attgtgacaa
     1681 ttgaagacgg gatcatggct ggcggagcag gaagtgcaat tttagaagca ctcagcaata
     1741 gaaatctgga tgtaaaatgc cgcatcatcg gcatcaacga cacattctcc acccacggca
     1801 cccagaatga actcaaaaaa ctcgaaggac tcactgttga aaatattgtg aaatctgttc
     1861 tggataaata aatgaaaaaa tcacgcgtca taacacttgg aaacattttg atcggtgggg
     1921 acaatccgcc ggtgatccag acgatgataa ctactccgat gaacaatttt gataaggcgc
     1981 ttgctgaggc ggaaacggct tttgagcttg gatgcagggt tgtgcggact gctttcaaga
     2041 acggtgatga agagcagagt ttaaaagata tggtcaaaag ttttaagggt gagatcattg
     2101 cagatattca ttttgattat cgtctggcgt tgatggcgat tgatgcgggg gttagcggtg
     2161 tgcggttcaa tccgggaaat gtcggtggaa aggaacgggt taaggaaata atagaatctg
     2221 caaagaaacg gcctgaaatg gcgatcagga ttggagtgaa cggcgggtcg cttgaaaagg
     2281 aaatacttaa taaatataac ggtgtaactt gggaaggtct tgtcgaaagt tcacttttct
     2341 gggctgatta tattgagcag gagcttggat acagaaactt taaaatatca gcaaaatcat
     2401 cgagtgttcc tgaaacgatc gccgcatgca gaatgataag ggaaaaatgt gatgcacctc
     2461 ttcatgtcgg aattactgaa gcaggcggag gcaaaagagg aataatcaaa agcactgccg
     2521 gactgtcaat tttaatttcg gaaggacttg cggacacttt cagagtgtcg ctcactgcat
     2581 caattgttga agaaattcag acagctaaca caattttaaa atcacttgga cttttaaaaa
     2641 ccggtgctga catcattagt tgtccaacct gcggaagaac tcacggagca gtgatcgaat
     2701 actacaacaa acttgaaaaa tggttcaatg aacagatctg gtggatgaaa ccggctctga
     2761 aagttgccgt catgggctgc gaagtgaacg gaccgggaga agctcgtgaa gcagatcttg
     2821 gaatagcttt aggaaaaaac tgtgcccttt attttgaaaa aggaaaaatc atcaataaat
     2881 tcaacgatca ggatgaagcg tttgaacatc ttttgaaaag gatctctgaa aactggaatt
     2941 gacaatcagg agcaaatcat gaaaaaaacc atccttgttt taattattgc gggctttctt
     3001 tctttttcct gcagcaacgg cagttctgat gaaaacccgg acttggaaca gaatgactcg
     3061 tccgctatta atgatgaaga ctcatctgac actggtgaca ccggaaattc aggtgattca
     3121 ggaaattcgg ggaattctgg tgacactggt gatactggaa acagcgggaa ctcaggggat
     3181 tcaggaaaca gtggtgactc cggcaactca ggaaataccg gtgacacagg aaattccggc
     3241 aacnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngaaaaag caatatatgt
     3361 cagcggtctt agctatatgt tcccgaatca ggaaaacgat caggacatat tcatcgcagc
     3421 cgttgatttt cagggcgaag ttctctggca tcatttcatc ggaactgatg aagacgactg
     3481 gtcatacgga cttgatgcgg ctgatggaaa actttttgta gtaggaataa ctcaaggtat
     3541 catcgaaggt gacggctccg caatctcaaa tgaagacgga gctattcttt tcacggaaga
     3601 atgatattca tttaaaaaac cgtaataata catccgcttg aagatttttt ttcaattgat
     3661 tcatcgctct cttcatcatt taattcatca gcttctgaga tagaatcggc atccggctct
     3721 tcttcagttg cttcacattt ttctccgccc cactcaccaa tgatgtcaac tgtcactttt
     3781 gtctttgaaa gatcaaattt aatttcccct ttcgcttttt catcgttcca gtagtcggca
     3841 actgtgcttg gaatcctgta tttggggtaa tatgccggag cgacatgtat cccaagccgg
     3901 tgacctttgt caaagattat ggatatgtag ccggcgttaa attccatttc ggtcaacgcc
     3961 cctttctcaa tcagagattc attttcaaga ccgttacgga atcttacttt ctgagcacct
     4021 tcaagcatca gatattcagt tccgtcagga tagatatcgg taaatatcac aaccatgtca
     4081 aaatcttcaa catcagccgc aaaaactgca tgaaaaccga tcgcacctgt aatttcagtc
     4141 ggctctaaaa acggctctgt gataaatgac atgacatcag tccgttctct atattcagaa
     4201 acatcacaag gtcccggatt tattgtcaga ttattaccac acaaagaagg gaaaggatca
     4261 ttcaggtcaa acttcagaat gaattcacct tcaccgcagt cacccgcttt taaaacgaga
     4321 tcggctcctg ccgacagaga aagttccgat gcatcatccg gaggaaattt atcagccgtt
     4381 ttccattcat ttcccggagc attttctttt ttcacatcac ctatcgtgta gtatgtgact
     4441 gtcgccattt caggtttttg cgtatatgac ggccagaaat aatggataag atatgcgaaa
     4501 accggatcat cttcaccttc atagtgtttt gcggcattct cagggaatgt aagctctccg
     4561 gagatgtttg tcatgaactt attgtgatcc cacggaccga taacaagatg ctgatcagta
     4621 gctcctgcct cagtaaaaag acgaaaagtg tcaatctggt actttccgaa catgtcaaac
     4681 catccggcaa ggtgtgccgc aggaatattt agattctcct ttttcttaaa gatatcggtc
     4741 acatcccagt aatcattgct tttgctgtac atgtcgccgg tcatcatatc atctatgaaa
     4801 aacgatgctt tctgatcgtt gagccatgtt gtaattgcat gttcccttaa aattccgccc
     4861 ggaaagaaag tcccgttgta gagatctccc gatgcaactg ttattatctg ataaataagt
     4921 cccggtttct catcaagtcc cgccatgaaa tactggacaa gtccttctgc cgatcctcct
     4981 attgttgcaa ttttcccgtt gcaccacggt tgatcagtta tccatttaag agtgtcatat
     5041 ccatccatgt gttcacccca tccttcggta tgaaagactc cgtgagtccc ttcagaaccg
     5101 aaagttcctc tgaatgactg cgcgatgaaa acaacattgg aatctgccat attaccgcct
     5161 gattcttctt tctgtgtcct gtcataagga gatctgttaa gaattacagg atagctttct
     5221 gtctgataat tatccggaaa atagatgtca gtttcaagac cggtcccgtc actcatcggg
     5281 atcattattg tctttttaac aactcctgaa agctgcatgg acactgtaat taaaactgtt
     5341 agaaaaatca cttttattac tttgctatgc atataaaccc tccaaaaaaa agacaaaaag
     5401 gatggtaatt aagaaaaatg ataatcctga caacaggttt ttcaagggaa tcaagagatt
     5461 attttaatat taccggagaa tgacacccca ttcaagcact gaaaatccct ctctgacaaa
     5521 tggctctata acaggttctt caagatgggc tacacatgtc gaagtctctt ttacagcata
     5581 tgaaagccgc tgcacgctgt cgggttcaat tgaaatgttc agttttacga ctggatctat
     5641 ctctgccgca gtctgcggat aaatgttgta gcatcctccg tcaagacggg gtatccacca
     5701 ttctatgaag tcttctattt cacgaccggc aaacccataa gcactcagat ttattctgaa
     5761 aaattcttca agatctccat tttcaacggt ccagccattt ctatattgga aaacatcggg
     5821 gttctggctc tcatagaaca gatatccgta ttttccatct atcgtaccgt ccgattcaac
     5881 tctcacattc caccctgttc catattcagg gatagaggtc gtcacatgtc caccctgagg
     5941 aaaatcaagt gtaatatcaa gatagaccgt ttcttccgga tacagataaa tattgggttt
     6001 ttccatctgt gcttcggccg gaaccctgag ttcgacataa tttgagttca cagtgacatt
     6061 ttcattatat tcagttatct cttcatcata atatgaacca tcctgatctt taaaagcgaa
     6121 agtgtaatca ccgtcaggca gatcgattac gaatcttcca ttaccatccg ttttaaaaca
     6181 tcctacaaga gaattatcct tataaacttt gacatctgca tcatatagag tgtacggccc
     6241 gaagtaatac tggatcatga agatagcccc gttaagatgt tcaggcatga ccggatcaag
     6301 atctacattg atggtatctc cgaaaacagc ataatcagtt tcggtaaaaa atccttcagc
     6361 agtctcataa acaaaatatg aaggtgtccc tccgtcataa ggattctctt caggaacttc
     6421 cagtaatttg cttccttcgg tctccattgt cagtgtttca agcagatcgc cccaaccgtt
     6481 tgagtgaaca gcgagtgaaa tatctttcgc gataagctcc aacccggtgc tgtcgtattt
     6541 cacattaaga tttttacatt ctccggaatc ggtatcctca tcgttctgtt catcctgtga
     6601 ttcatcaaca gtttcgtctg ttatttcatc aacagtttca tcaaccgttt catcagatat
     6661 ctcatcagtg gtctcatcat ttatttcgtc agatgtttca tcatcgttca cagattcatc
     6721 attcgttcct tcattatcac taaccgtatt cacctcattt ccacatgaga ccaacataaa
     6781 accggcaaag atcattgcaa aaaacataaa tcttctcatc tttttctccc ttaaaaaacc
     6841 ggataaataa cgacttttag taacagtaat gtattatatt tattacagga acacaaaaat
     6901 aaattgccag attttataaa acagctagaa aaaaagttaa gcgaaatcca tattgtttgt
     6961 catccatctt atgaactggc agcatcccca tggtgcatat aatcctaagg tgacaactgt
     7021 caggatcaga ataagaatat aagttccgaa aaagccgaga cctgtccctt tgaaaacaag
     7081 ttttctgcct gttacaaatg tgttttccgc aatccatctc atctgagcgg aaactgccca
     7141 gggaagaaaa agaccgaaag tgatcgcagt tacaactgtt gtccagatca gaagccacag
     7201 aaaacccagc cctgttcctt taaactcgaa atttcctacc gtcatgatca actccttaga
     7261 aaaattaata atatacatct tagtgatgaa cgaaaaagct taatgtttta aaactgtttt
     7321 gtcaaaattt ctgagtgttt aaaaacctgg aaacatccaa caaaagtaaa tgattccctg
     7381 gatcaccacc attttttctt tttgaagaag ataaccattg aaacaaagat gactgacatg
     7441 agtccaagtg aaataaaata tccgtaactc cacttaagtt ccggcatatt ctcaaaattc
     7501 attccgtaaa ctcctgcaat aaatgtcaga ggaatgaaaa ttgttgaaat aattgtaaga
     7561 actttcatga catcgttcat tttaaagctg acacttgaaa gatacagatc aagcaaacct
     7621 gttgatatat ctctgaaagt ctcaacagtg tcaattacct gaactatatg gtcataaaga
     7681 tcgcgcagat atatttcagt cttttgttca atgacatcta tttcccccct ttccagccgg
     7741 cttacgacat ctctcaaagg ccagaccgat ttccttacca gaaaaatatc tctctttaat
     7801 ctgtatattt ttccgagcgt atttgaggca ggattgctca tcagctcttc ttcgagcact
     7861 tctatttctt caccaatttt ttccagtatt tcaagatagt tatccacaat tacatcgaat
     7921 atttcataag caagatagtc ggcacccgaa gatcttgtcc gcccttttga cttacggatc
     7981 ctttcccgca gagaagcaaa aacttcactc tctttttcct gaaacgaaag tactttgttt
     8041 tttctgacta tcaaactgac ctgctcagag acaaccacag aacttttacc gtcaaaaaaa
     8101 agcattttaa aaacaataaa gaaatgatca tcaaaaattt caattttagg tctctggttt
     8161 gtgtttaaaa tatcttcaag aaccaatgga tgaacatcaa atattttccc tatttcatca
     8221 atcttttcaa catcatgaag accgagaaaa tccagccatg aaacattttt agaatctctg
     8281 aattcagcgc attcatttat atcagcaaca cttttctcag tgaaatgatc tgcactgaaa
     8341 tccatgatgt tgatatccat tttctctctt ttcttttcac ctatgaaaac cagacttccc
     8401 ggactcatcc ctttcttttt tgatattttc ataataatcc tcctgaaaaa cagcattatt
     8461 atagtaattt agacattttt atgcaaatct gtcgggat
//
LOCUS       DAOOYB010000118         8462 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_9296_length_8462_cov_11.626621, whole genome shotgun sequence.
ACCESSION   DAOOYB010000118 DAOOYB010000000
VERSION     DAOOYB010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8462
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9296_length_8462_cov_11.626621"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1429)
                     /locus_tag="PL056_08045"
     CDS             complement(<1..1429)
                     /locus_tag="PL056_08045"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPM47005.1"
                     /translation="MKKSFFQAVLTIVLIFAAGCGSSPAKLFEKGNSVKATDKVKARG
                     LYEQACEQNHFEACHNAALLWQNGDDINEENKKARVLFTKACEGQFNESCLILADMWV
                     HGKGGVYKDSMSAFNLYKVLCDRGEGKGCRGMGEMYSKGAASLDRDVKKAIELFEMAC
                     EKGDMKTCVDLGQTWSKKFEDPEGYEKVELFWGKACMSGMIDICEKFADMIVFRKEKE
                     ERLPLSRKYYEKACEGKSREACYKLARVIVDNVENGEMEAKPFYDKGCEMDHAESCYY
                     IGVYLSQAYGETLELDKLKEMHSFYDKSCTKGFDPACSSAQSLKALIDEQEEAIRRAE
                     EEKRRIEEEKRKEEERQATAEAERLRLEREKAELDAKLQEEEERKAREEEERREKEEM
                     DRRRKEAEERKSMDEEEEEEEVVPSKEDEDFEDEEEDVRPAPKKEEPASEDFDDGLDD
                     FEEEEEEEEEKPAPKKEKKAKKSAEE"
     gene            complement(1517..2644)
                     /locus_tag="PL056_08050"
     CDS             complement(1517..2644)
                     /locus_tag="PL056_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47006.1"
                     /translation="MFSFTYFLNPFFVAAVIVAILFPFVRKGFPVMKIVVFNLTVILV
                     LAGLFDATATTFFAPPYNNFRTNSSYYHHGLKPNMTEKTRWFNEYTVHTNSLGFLDRE
                     QRDVPLKKNGERFVFIGDSFTEGVNAEWDQTFPGIVAKELEKEGIELLNAGCISYSPK
                     IYYLKMKYFIEKGLEMDDLFVFIDISDVQDEIEYAYFEPSEPVFLLSDINLFMYQNSY
                     SYRSLYDRFFYFQENPRHKDSPYWGGAFYPVRHLWSMDMEHYNKWGKEGLELSGKYME
                     LLFNLTREHNIKLHVAVYPWKFHIRNRNLDSIQVIFWKKFCEDKGIEFINFFPDFINV
                     EDVVEMERKWFAKEDVHWSSEGLEKMAQSVINHFKKVKILK"
     gene            complement(2669..5104)
                     /locus_tag="PL056_08055"
     CDS             complement(2669..5104)
                     /locus_tag="PL056_08055"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPM47007.1"
                     /translation="MNRFRDHFVQFCFTIFLVLPPFLLGGVYSHTGTNLLIISILSAV
                     SLCFFSYLLIYRKPLLISSVFFPLAAILLYAVFQLVPLPLTVLKAISPKAHFFHLLEG
                     SGARPLTMSVPDTVYSIMRVTVLTLFSIIVARTVFLGKRKWKQILIDTIIYISSIVIL
                     VSAALRIVNAQTWLYGTLRHPGFLIDPILINPNHAAAFFGISAILSLTSIYSHDFSRK
                     KIFYGSLFFIHSIAVVATLSRGGILAFAASLIFFLLIKRTESEKETGKAKLYLAGFSL
                     IFALFVVFQTGYALLEKEFDFEREGYFSKIEEMGTVRDYIGDFFLTGSGLGSFSKVYS
                     YYQNDPERKFTELENEPVQFMLENGAFFGLFIFTFLVWIILKGRKDVKRNSGFISVFV
                     FIFLHNTIDFNLHNFSTLFPLIAIVAMTVQPIELSGRKRAGVIFFCIAASLSLLVMTL
                     SESGRKTAGYSENFSYDELVYNYPADYSVPMGKAIEKLNSPQKEESGIAGKYATAAML
                     KAPQYYFTHYLAGNYMLRLGSIEQALVFYRKSIELSGGKYPVLLRKIYGDLKSAGISG
                     KMTDIISISDGNISELERFLFEVSRTDQSSLKFIHSNEELFFLSAIRSYLDKKDFDSA
                     RKLILHVESAKKEMPDEKKGRLFIYKGLVYENSKDYRQAFEHYSKGAALTGGFFDHLQ
                     AAYCSLHLDRTAIDKAEKGMKTALLKSSRNIAHYYKWLSRREFMDNNYADGFKYLEKA
                     VDVSKNPNWRLELANTYSRRGMHYIALKTIMILKKEQPDFSRELINKRIEEEKKKLSD
                     KEQKNFNELMLKN"
     gene            complement(5114..6664)
                     /locus_tag="PL056_08060"
     CDS             complement(5114..6664)
                     /locus_tag="PL056_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47008.1"
                     /translation="MIKNLVFFLLLLISFSIYSEAVDIKEITDPAILEQLNNDTTMIP
                     MQKGALFVPYIVDPLLEPHYTIFKDDDFFADAEPGRRIPLFEGEYTVYIGSGPLDMQT
                     EMKVYILNERTTVVIPSWSALIIKTIDQNNNDIRESYTLVNEKTKIQVGAGSGADVLR
                     GEKEQVWILPPDLYRVAKRGESPYSFQNFVTVRTQKGKLSTVQMVFEEKTRAVLGGGE
                     VISDTEDRKYTGNWSFKANISANFSLVNSGYIKGGSGDSNDFTFGSTLNTSIIYDDRN
                     FLFINRLEINELFQKPDGEKLRFVKDLMKFDSSFIYRINQYVGPYVSVRVRAPFGYKY
                     LKTSDDTDEPQVAVVEENGDTRNIGRGEVFTYSKSFSSTILQEGTGLNADYIYGNSFK
                     ITGRVGWGFRQDFNPFSYDVSKLESDYTVTRVPYFKNLTGPEFYLYLSWFPLSFLEIK
                     EEFDALLPVDDVNQFSFFSKTSAFLWISRFAAIQYDFEIEKSPSTNSSDTITSEHTLT
                     VQIYYNFL"
     gene            complement(6657..7259)
                     /locus_tag="PL056_08065"
     CDS             complement(6657..7259)
                     /locus_tag="PL056_08065"
                     /inference="COORDINATES: protein motif:HMM:NF015823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HPM47009.1"
                     /translation="MKKLIIITILITLFTGAGCSLFSSSAPAKHYYQIYYTPSKTLGK
                     KISGTVRIKTFETDKIYRIHNIVKRKSQNEIQYYTSRFWASRPTDMITDQIAKHVTET
                     ELFSDVIMQLDRRPDFVLTGRILALDLVDSNEKWFARVSIVFELRDYKSDRTVITHSF
                     DERREVSSMEVVHAVRTMGEIIEKEAEVFFNKIYEAMNID"
     gene            complement(7256..8236)
                     /locus_tag="PL056_08070"
     CDS             complement(7256..8236)
                     /locus_tag="PL056_08070"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HPM47010.1"
                     /translation="MQITGAQKRRVAIFFLFGIGIIILLTAVLVGNKLLKREDCYYSR
                     FTDISVAGLTEGSSVKFQGMNIGHVSAISIDHENTEVVQIEFCLKPGVPIKEGTMAQL
                     GNIGITGLKFLELQGGGQGKDIPVGGEIPSMKSGWDEITGKATVIAEKLESILNTVNI
                     AIKGLKKEDVESIVHNIDGISGSVNVILKENKNDIRSIVSRVDVLLKEVNSNMSNISA
                     ITKNVKDLTSPEGSITKTLLIVEDTVRELNKDYKEADIKDKIDKMFALVETTQKTIDT
                     VNLTLERSKENIDTSLTEMSEGMSNFSEFTRIIMENPSALFNAPKSGEVE"
BASE COUNT         2372 a         1885 c         1495 g         2710 t
ORIGIN      
        1 cctcttcagc acttttttta gctttcttct ctttcttagg agccggtttt tcctcctcct
       61 cctcctcttc ttcttcaaaa tcatcaagac cgtcatcgaa atcttcggaa gcgggctctt
      121 cttttttagg agcaggcctt acatcttctt cttcatcttc aaaatcttcg tcttcttttg
      181 atgggacaac ctcctcttct tcttcttcct cgtccatgct ttttctttct tcagcttctt
      241 tccttcttct atccatttcc tctttttctc ttctttcctc ttcctctctc gcctttcttt
      301 cttcttcctc ctgcagttta gcatcaagtt cagctttttc tctttcaagc cgtagtcttt
      361 cagcttctgc agtggcctgc ctttcctcct cttttctctt ttcctcttct attcttctct
      421 tttcctcttc ggcccttctt atcgcctctt cctgctcatc aatgagagct ttaagtgact
      481 gtgcacttga gcaggcagga tcaaatcctt ttgtgcacga cttatcgtaa aagctgtgca
      541 tctccttcag tttatcaagc tcaagtgtct ccccataggc ctggctcaaa tagacaccga
      601 tataataaca tgattcggca tgatccatct cgcaaccctt atcgtaaaag ggttttgctt
      661 ccatttcacc attctcaaca ttatcaacaa tgactcttgc gagcttgtaa caggcctctc
      721 ttgattttcc ttcacatgct ttttcataat attttcttga aagtggcagc cgctcctctt
      781 tttcctttct gaaaacgatc atgtctgcaa atttttcaca gatatcgatc attccgctca
      841 tacacgcctt tccccagaaa agttcgactt tttcataacc ttccggatct tcaaacttct
      901 ttgaccatgt ctgtccaagg tcgacacatg tcttcatgtc ccctttttca catgccatct
      961 caaaaagttc gatcgctttt ttgacatctc tatcaaggga cgcagcgcct tttgagtaca
     1021 tttcacccat tcccctgcaa cccttaccct ctccacggtc acaaagcacc ttgtaaaggt
     1081 tgaaagctga catgctgtct ttgtagacac cgccttttcc atgaacccac atatctgcaa
     1141 ggatcaggca cgattcattg aactgacctt cgcaggcctt tgtaaaaaga accctcgcct
     1201 ttttattctc ttcatttata tcgtcaccgt tctgccataa cagggcggca ttgtgacatg
     1261 cctcaaaatg attctgctca catgcctgtt catacaatcc ccttgctttc accttgtcgg
     1321 tagctttgac cgagtttccc ttttcaaaaa gttttgccgg gcttgaaccg catcctgcgg
     1381 caaagatcag aacaatcgtt aaaactgcct gaaaaaaaga ttttttcatt tgtattcccc
     1441 ctggatatca aaaatcataa taacaaactg gtaataattt aactattgta tctggcaggt
     1501 caacaatttt tcttaattat tttagtattt ttactttttt aaagtgattt atcaccgatt
     1561 gagccatttt ctcaaggcct tcggagctcc agtggacatc ctcttttgca aaccattttc
     1621 tttccatttc aacaacatct tcgacattaa taaaatcagg aaaaaagtta ataaattcaa
     1681 tgcctttgtc ttcacagaac tttttccaga aaatcacctg gatagaatca agatttctgt
     1741 tacggatatg aaatttccat ggatatactg caacatgaag tttaatgtta tgttcacggg
     1801 tcaggttaaa aagaagttcc atatattttc cggaaagttc cagcccctct tttccccatt
     1861 tgttgtaatg ttccatatcc attgaccaaa gatgtcttac aggatagaaa gctccgcccc
     1921 aatagggaga gtctttatgt cttggatttt cctgaaaata aaagaatctg tcatataaag
     1981 atctgtatga atatgagttc tgatacatga aaagattgat atcacttaac agaaagaccg
     2041 gctcagacgg ctcaaaatat gcatactcta tttcatcctg cacatcagaa atatcaataa
     2101 aaacaaaaag atcgtccatc tcaagccctt tttcgatgaa atatttcatc ttgagataat
     2161 agatcttcgg agaatagctt atgcatccgg cgtttaaaag ctcgattccc tctttctcaa
     2221 gttctttcgc aacaataccg ggaaaagttt gatcccactc cgcattcacg ccttcggtaa
     2281 aggaatcacc aataaaaacg aatctttccc cgttcttttt caacgggaca tctctttgtt
     2341 ccctgtcaag aaaaccgagc gaattagtgt gaactgtata ttcattgaac caccgtgtct
     2401 tttcagtcat gttcggcttc aaaccatggt gataatatga actgttggtt ctaaaattgt
     2461 tgtaaggagg agcaaaaaaa gttgttgcag tagcatcaaa aagtcccgca agaacaagaa
     2521 ttaccgtcag gttgaacaca actatcttca tcaccggaaa tccttttctc acaaacggaa
     2581 aaagtatcgc gacaataact gccgcaacaa aaaaaggatt cagaaaatat gtaaaactga
     2641 acaagctatt ctcctgtata tctgacaatt aattcttaag catcaactca ttaaaatttt
     2701 tctgctcttt gtctgaaagc ttcttttttt cctcttcgat tcttttattt atcaattccc
     2761 tgctgaaatc aggttgctcc ttttttaaaa tcataatcgt tttcagcgca atgtaatgca
     2821 tccctcttct gctgtaggtg tttgcaagtt caagccgcca gttgggattt tttgaaacat
     2881 caactgcttt ttcaagatat ttgaacccgt cggcatagtt gttgtccata aactcccttc
     2941 ttgagagcca tttgtaataa tgcgcgatgt tccttgatga tttgagaaga gctgttttca
     3001 tccctttttc tgctttatct attgccgtcc tgtcaaggtg gagtgagcag tacgccgcct
     3061 gaagatgatc aaaaaagccc cctgtcagag cggcaccttt tgaatagtgt tcaaatgcct
     3121 gtctgtaatc tttgctgttt tcatatacca aacctttata gataaaaaga cggccctttt
     3181 tttcatccgg catctctttt tttgctgact caacatgcag aataagcttt ctggcagaat
     3241 caaaatcttt tttatcaagg tagctcctta ttgcagaaag gaaaaagagc tcttcgttgc
     3301 tgtgaatgaa ttttaaagaa ctttgatccg ttcttgacac ctcaaagaga aatctttcaa
     3361 gttcagaaat atttccgtca cttatggaaa ttatatcggt cattttgccg cttattccgg
     3421 cagatttaag atcaccgtat attttcctta acagaacagg atatttaccg ccgctaagtt
     3481 caatgctttt tctgtaaaac acaagcgcct gttcaattga accaagacgg agcatataat
     3541 tgcctgcgag atagtgggta aaataatact gcggcgcttt cagcattgcg gccgttgcat
     3601 attttccggc aattccgctc tcctcttttt gaggtgagtt gagcttttcg atcgctttgc
     3661 ccatcggaac agaatagtcc gccggatagt tgtaaacaag ttcatcatac gaaaaattct
     3721 cactgtagcc agccgttttt ctgccgctct ctgacaaagt cattactaaa agcgatagcg
     3781 atgccgcaat acagaagaat attactccgg ctcttttcct tcctgaaagt tcaatcggct
     3841 gaacagtcat ggcaacaatg gcgataaggg gaaaaagtgt ggaaaaatta tgcagattaa
     3901 agtcaatcgt attgtgaaga aaaataaaaa caaataccga aataaacccg ctgttccttt
     3961 ttacatcctt tctccctttc aggataatcc acacaagaaa tgtaaatatg aacagcccga
     4021 aaaaggcccc gttttcaagc atgaactgaa ccggctcatt ttcaagttca gtgaactttc
     4081 tttccggatc gttctgatag taggagtaaa cttttgaaaa acttccaagt cctgaaccgg
     4141 taagaaagaa atcaccgatg taatccctga ctgtgcccat ctcttcgatc ttgctgaaat
     4201 acccctccct ttcaaaatcg aattcctttt ccagaagagc ataccctgtc tgaaaaacga
     4261 caaaaagagc aaaaattagt gaaaatccgg caagatagag ttttgctttt cctgtttctt
     4321 tttcagattc cgtccttttg atcagaagaa agaaaataag agaagccgca aaagcgagaa
     4381 tgcctcccct tgaaagagta gctacaacgg caatagagtg aataaaaaac agcgatccgt
     4441 aaaatatctt ctttctgctg aaatcatgag aatatatcga tgtcagagaa agtattgcag
     4501 atattccgaa aaatgcagcg gcatgattgg gatttataag tatggggtcg ataagaaatc
     4561 ccggatgcct gagcgttccg taaagccagg tttgtgcatt cactatcctt aatgcggcag
     4621 aaacaagtat tacaattgag gatatataga tgatcgtatc gatcagaatt tgtttccact
     4681 ttctttttcc aaggaatacc gtccttgcaa caatgatcga aaaaagtgtc agaaccgtaa
     4741 cccgcatgat cgaatatact gtgtcaggaa cagacattgt cagaggtctt gctcctgacc
     4801 cttcaagcag atgaaaaaaa tgtgctttag ggcttattgc cttaagaaca gtcaatggaa
     4861 gtggaacaag ttggaacaca gcataaagaa gaattgcagc aagaggaaag aaaacggatg
     4921 atatcagtag cggttttctg tagatcagca agtaggagaa aaaacagagc gatacagctg
     4981 aaagaattga aataattaaa aggtttgtgc cagtatgtga atatacaccg ccaagcagga
     5041 acggagggag aacaagaaag atcgtaaaac agaactggac aaaatgatcc ctgaatctgt
     5101 tcatgacaat tcctcacagg aaattataat aaatctgcac cgtcaaagtg tgttcgcttg
     5161 tgatggtatc gcttgaattc gtgcttggag atttttctat ttcaaaatca tattgtattg
     5221 cggcaaatct tgatatccac agaaacgctg aagtctttga aaaaaagctg aactgattca
     5281 catcatcaac agggagcagc gcatcaaact cttccttaat ttcaagaaaa ctgagaggga
     5341 accatgacag ataaagataa aattcaggcc cggtaagatt tttgaaatac ggaacccttg
     5401 taacagtgta atcgctttca agttttgaaa catcgtatga aaaaggattg aaatcctgtc
     5461 tgaatcccca tccgacacga cctgttatct taaaagaatt gccgtaaatg taatcagcat
     5521 ttaagcccgt tccttcctgc agaatggtag aggaaaatga ttttgagtaa gtgaaaactt
     5581 cacctcttcc aatatttctc gtgtcaccat tttcttcaac aactgcaacc tgcggctcat
     5641 cagtatcgtc tgatgttttc agatatttgt acccgaacgg tgctctaacc cttacagaca
     5701 catacggtcc gacatattga tttattctgt atatgaacga gctgtcaaat ttcatcaggt
     5761 ctttcacaaa acggagtttc tcgccgtcag gtttttgaaa gagctcattt atttccagcc
     5821 ggtttatgaa aagaaagttc ctgtcatcat aaattatgct ggtgttcaga gtcgaaccaa
     5881 atgtaaaatc attggaatca cctgatccgc ccttgatata tccggaattc acaagagaaa
     5941 agttcgcaga aatgtttgcc ttgaaagacc agttgcctgt gtactttctg tcctcggtat
     6001 cgcttatgac ctcaccgcct ccaagcactg cccttgtctt ttcttcaaaa accatctgaa
     6061 ctgtgctcag ttttcccttc tgggtccgga cagttacaaa attctgaaat gaataagggg
     6121 actctcctct ttttgcaact ctgtaaagat cgggcgggag tatccagacc tgttccttct
     6181 cacctctaag tacgtcggct cccgagccag ctccgacctg tatctttgtc ttttcattga
     6241 ccagagtata tgactctctg atgtcgttat tgttctgatc gatcgttttt atgataagcg
     6301 cagaccacga aggaattaca acagttgttc tttcgttcag tatataaact ttcatttctg
     6361 tctgcatatc aaggggacct gaacctatgt aaacggtata ttcaccttca aaaagaggta
     6421 ttctcctccc cggctcggca tctgcaaaaa aatcgtcgtc tttgaatatt gtgtaatgcg
     6481 gttcaaggag cggatctaca atgtagggaa cgaacaatgc acctttctgc atcgggatca
     6541 ttgttgtatc attattcagc tgttcaagta ttgcgggatc tgttatttct tttatatcaa
     6601 cggcttcact gtatattgaa aaggagatta ggaggagtaa aaaaaaaacg agatttttaa
     6661 tcaatgttca ttgcttcata aattttattg aaaaatactt ccgcttcctt ttctattatt
     6721 tcccccatcg tccttacggc atgaacgact tccatgcttg aaacttctct tctttcatca
     6781 aacgaatggg tgatcaccgt tctgtccgat ttatagtctc tcagttcaaa aactattgaa
     6841 acccttgcaa accatttttc attgctgtcg acaagatcaa gagcaaggat ccttccggta
     6901 agaacaaaat caggacgcct gtcaagctgc attattacat ctgaaaagag ctctgtctct
     6961 gtaacatgtt ttgctatctg gtcagtgatc atatctgtag ggcggcttgc ccagaaacgg
     7021 cttgtatagt actgtatctc gttttgagac tttcttttta cgatgttgtg gatcctgtat
     7081 attttatcag tttcaaatgt ttttattctt actgttcccg aaatcttctt tccaagtgtt
     7141 tttgatggag tgtagtatat ctgatagtag tgttttgcag gggctgatga tgaaaaaaga
     7201 gaacatcccg cacctgtaaa aagcgtgatc agtatcgtta ttataattaa ctttttcatt
     7261 ctacctcccc cgacttagga gcattgaaca aagctgaagg attttccatg attatccttg
     7321 taaattcact gaagttgctc atcccttcag acatttcggt cagcgatgtg tcaatattct
     7381 ccttgcttct ttcaagtgtc agattgacgg tatctattgt tttctgagta gtttcaacca
     7441 gcgcgaacat tttgtcgatc ttgtccttta tatccgcctc tttataatct ttgttcagtt
     7501 ctctgacggt atcttcaaca attagaagag tttttgttat tgaaccttca ggcgaagtga
     7561 gatctttcac attttttgtg atcgctgaaa tgttgctcat gttgctgttt acctctttaa
     7621 gcagaacatc aactctcgaa acaatgcttc ttatgtcatt cttgttctct ttgagtatga
     7681 cattcacaga gccgcttatt ccgtcgatgt tatgaactat gctttccaca tcctcttttt
     7741 tcagcccttt tatcgcgatg ttcactgtgt tgaggatcga ttcaagcttt tcagcaatta
     7801 cagttgcttt cccggtgatc tcatcccagc cgcttttcat tgacgggatc tctccgccga
     7861 cagggatgtc ttttccttgt ccgccgccct gaagttcaag aaatttgagt cctgttattc
     7921 cgatgttacc aagctgtgcc attgttccct cttttatcgg cacacccggc ttaagacaga
     7981 actctatctg tacaacttca gtattctcgt gatctatcga gatcgccgaa acatgaccaa
     8041 tgttcattcc ctgaaatttc acagatgagc cctcggtgag acctgcaaca gaaatgtcag
     8101 taaatctcga ataatagcag tcttctcttt taagcaactt gttcccgaca agaacggcag
     8161 tcagaagtat gattatccct attccgaata gaaaaaaaat tgcaacccgt cttttttgag
     8221 caccagtgat ctgcatttca ccccgaaaat ctcaattcac aagattgtaa agataaattt
     8281 aattgttgtc aataaatagc aagtaatacc acaagtaata ctaaaaaact tgataataaa
     8341 gggcttggaa tctattatta ccgtcgtttt gtatttttaa catattgagg ttgccatgtt
     8401 tgatgtaaag ctgatactgg aaaacaaaga caaggtgttg aaaagtctga tgaatcgtaa
     8461 tt
//
LOCUS       DAOOYB010000119         8363 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_9457_length_8363_cov_9.680549, whole genome shotgun sequence.
ACCESSION   DAOOYB010000119 DAOOYB010000000
VERSION     DAOOYB010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8363
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9457_length_8363_cov_9.680549"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..780)
                     /locus_tag="PL056_08075"
     CDS             complement(<1..780)
                     /locus_tag="PL056_08075"
                     /inference="COORDINATES: protein motif:HMM:NF012389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HPM47011.1"
                     /translation="MGFLDTLNVKCINELAIREKRVFIRTDFNNPFDNDGKIDIERIG
                     KTLPTIKHAVEHNSRVIIASHMGRPEIERDPAFSLEPVAAALAELLDHDIFFFEDCVG
                     MGAQQMVRDLKPGHILVLENLRYNEDEMKNSSSFARELAKMCDIYINDAFGVSHRKEA
                     SVNALPQIMDTKGMGFLMKKEIDELSKFSGLKRGDGFCFIVGGSKVSEKLSTVRYMLE
                     VADRIIIGGSMAHTFLAAKGVRLGSSKVEEDKIGTAKELIKG"
     gene            complement(804..1802)
                     /gene="gap"
                     /locus_tag="PL056_08080"
     CDS             complement(804..1802)
                     /gene="gap"
                     /locus_tag="PL056_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018132098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HPM47012.1"
                     /translation="MTKVRVAINGFGRIGRAVFRIADKSEKIEIVAINDLTDPKTLAH
                     LLKYDSVHGVWDHEVKAGEASISVDGKEIMIIAEKDPSKLPWKELNVDAVLECTGLFT
                     ERAKADLHIQAGAKKVLISAPAKGEDLTVVLGVNDSLYDKQKHNVISNASCTTNCLAP
                     ITHVINKHFKIKHGFMTTIHSYTNDQRILDLPHKDLRRARAGALSMIPTTTGAAKAIG
                     LVIPELKGKLDGISIRVPTPNVSVVDVVFVVEKETTAEEINKLMKEYAEGPMKGILRY
                     CDEPLVSKDFNGDPHSSILDAKNTAVMQGTMIKLLSWYDNEWGYSNRMYDVLTKLF"
     gene            complement(1824..2843)
                     /locus_tag="PL056_08085"
     CDS             complement(1824..2843)
                     /locus_tag="PL056_08085"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPM47013.1"
                     /translation="MVRKRVMVTELDEGDIIIPDSNLYFLVRKSAVKTGKNSQRYMDL
                     ELFDGINQVNGKVWDINPETEAALQPGNVVKILTGKVTKYQTNTQVVITDAETVPPEK
                     IDSECRGILPESVFSASELSERWENTCSKLKDDHKKVIENFENSGKVWELFTAIPAGR
                     SMHHAYRRGLWEHSMNVAEMAFDIAAKYEKRYPVDVSLVVLASLIHDIGKVFEFQLNT
                     ATALVERYSDRGKLLGHIYMGATWVEKLLSTCCPDDSDLKVELLHIMLSHHGEYEFGS
                     PKKPKTMEAIIVSMCDNLDANLDAVNIGFSGEMEGNWTKQIYMMQRAFYKREQGSDLN
                     KDIND"
     gene            3070..3651
                     /locus_tag="PL056_08090"
     CDS             3070..3651
                     /locus_tag="PL056_08090"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPM47014.1"
                     /translation="MSDVLKEQVLNEAKKLFFVYGFKKVSMDEIAAACKMSKKTIYQL
                     YSSKDELIRECVDRISTEKVSAIHRILDSEPLVSGELNGMFEIFGTINQDVSEAMMRD
                     MSYFPDVWNMIEEKRMTVFARMGDIIEQGKLDGSINPSLNVDLFLRIFMGILQRFANP
                     AMFAELNLRPSEFIEQMKMVVFEGILTVKARRQ"
     gene            3653..4630
                     /locus_tag="PL056_08095"
     CDS             3653..4630
                     /locus_tag="PL056_08095"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPM47015.1"
                     /translation="MKKYLVIPIIFIFAVFSSCQKVEPTVLTLSGTVESKDVRISSLV
                     GGRITEVNYDEGDEVKKDSIIAKIDCRDYELQLAQAETVISGAKAKLRLIKSGARKED
                     IASASESNKQAQIALDKANKEYGRFEKLFETGSITEKELDDIKVVRDRAQSQFDQSRH
                     ILEKLQNGAQIEEIDSVIASVKQAEASKAILLKKISDCVILSPSDGTVLHRLAEPGEI
                     ASPGMAILTLSDISKVRIRAFVPEKELGHIKNGAEVQLFIDTFPKISFSGKIASISTE
                     AEFTPKTIQTADERVKTVYEFKVIAENKDRIFKPGMPVDIVIDKTEAVF"
     gene            4627..5544
                     /locus_tag="PL056_08100"
     CDS             4627..5544
                     /locus_tag="PL056_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPM47016.1"
                     /translation="MSTVLEIKNASFLYGMTKALDDVSFEVKKGETFGMIGPDGAGKT
                     TLIRLFCGLLKPSSGNCTVLGFDTVKEKYQLIPKIGYLSQKFSLYTDMTVSENINFFA
                     DIHKVRNYSAKKNELLEFTGLSNFKDRLAGALSGGMKQKLALSCTLIHTPEIIFLDEP
                     TNGVDPVARRDFWKILKDISKQGVSIIISTPYIDEAEKCDRVAFMNRGSLIACDTPAG
                     VKSRYRYKLFEIVSLDVQNTKTLLEKCDRISTVQIFGNTLHAGTERSEDDSFIKDLAG
                     KVEGVMRIKEIKPSLEDVFIDLLENRNSI"
     gene            5541..6302
                     /locus_tag="PL056_08105"
     CDS             5541..6302
                     /locus_tag="PL056_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPM47017.1"
                     /translation="MNGNEFAIEVTGLKKNFGSFQAVKKVSFNVKKGEIYGFLGANGA
                     GKSTTIKMLCGLLEPDGGKAVVAGTDVEHDPEGVKKRIGYMCQKFSLYEELSVSENLF
                     FFGGIYGLEKIHLKKRIEEIAQILDLSAVINVPSGELPKGFQQRLALGCAIIHEPEII
                     FLDEPTAGVDPIQRRNFWNIINDLSVNGTTVFVTTHFLDEAEFCHRISFIVEGSIVAD
                     DTPSGLKEMLKREKSEFNREPTLEDVFIFLAGGDK"
     gene            6299..7423
                     /locus_tag="PL056_08110"
     CDS             6299..7423
                     /locus_tag="PL056_08110"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPM47018.1"
                     /translation="MSVKPVVKKEFIQIARDTRTLFMLAFFPATLLLVFGYVLSFDVK
                     EIKLGIFDNDRSEESSTLISAVSSTEYFIIGRVFENRSRIDSFLDDGTLDAVIIIPED
                     FGKNISSGKGSPVQVMIDGSDGRSAGIVQGYIAGFINSYSQKVVSSYISGAGRKVEPP
                     VNIEPRIWYNPELKSSIFLVAGLIVFILMITGVISTALSVVREREKGTMEQLLVSPLS
                     ATAVIIGKTIPYLVISLLSTVIILVTGYLAFGVEVKGSYLMLFVSSTLFIMSALAQGI
                     LISTITTSQQVAFFAASLSSLLPALLLSGFIFPIAGMPYIIQAITTIVPAKYFVNLLR
                     GIIMRGSGFDTGLSDLAALTIFSFVLLTISVIRLKKVKLV"
     gene            7426..>8363
                     /locus_tag="PL056_08115"
     CDS             7426..>8363
                     /locus_tag="PL056_08115"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPM47019.1"
                     /translation="MLKFMIWKEFLQLKRDKKMFGIIFMAPVIQLIVLGYAANFDVER
                     ISTAVCDYDRSNESAQYLDRYFSGGYFERTINLNKSGDIDKLLEKGEIQAAIVIPSGF
                     GRNIAKGTQTDVQLIINGSDTNYATNAFNYAAQITARFSTKILLEKMAMAGMNSIPQV
                     KLEQRLLYNPELKTKNFMVPAIMGLILMIITVMLTSMAIVREKEMGTIEQLIVTPIKK
                     GPVLAGKLIPFLIIGLIDVALVTAVAIFLFRIPFKGSFFLLFCGAILFLFNTLGLGLF
                     VSTVSATQQQAMMTVLFVIMMPFIYLSGFVFPVENMP"
BASE COUNT         2404 a         1756 c         1748 g         2455 t
ORIGIN      
        1 acctttgatc aactcttttg ctgtccctat tttatcttcc tcaacctttg aagaaccgag
       61 tcttacccct tttgctgcaa gaaaggtatg tgccattgaa ccgccgataa ttattctgtc
      121 tgcaacttca agcatgtatc ttacagttga aagcttttcg gaaactttag atcctccaac
      181 gatgaaacag aatccgtcac ctcttttaag tcctgagaac tttgaaagtt cgtcaatttc
      241 ctttttcatc aaaaatccca tcccttttgt atccattatc tggggaaggg cgttgacaga
      301 tgcctctttc ctgtgcgaaa caccgaaagc atcattgatg tagatatcac acatctttgc
      361 aagctctctt gcaaaagaag aggaattctt catctcatct tcgttatatc tcaaattttc
      421 aagaacaaga atgtgtcccg gcttaagatc tctgaccatc tgctgggcac ccattccgac
      481 acaatcttcg aaaaagaaaa tgtcgtgatc aagaagttcc gcgagagctg cggcaacggg
      541 ttcaagtgaa aaagcgggat ctctttctat ttccggacga cccatatggc ttgctatgat
      601 aactctcgag ttgtgctcaa cggcatgttt tatagtgggg agagttttcc cgatgcgttc
      661 aatatctatc tttccgtcat tatcaaaagg gttgttgaaa tcggttctta taaaaaccct
      721 tttttctctg atcgccagtt catttatgca cttcacattt agagtatcaa gaaagcccat
      781 atgagtctcc gttaattgag ttatcagaaa agttttgtca gaacatcata cattctgttg
      841 ctgtatcccc attcattgtc ataccagctg agaagtttta tcattgtccc ctgcattact
      901 gcggtatttt tcgcatcgag tatggatgag tgaggatcgc cgttaaaatc tttggaaaca
      961 aggggctcgt cacagtatct gagtatacct ttcatcgggc cttcagcata ttctttcatc
     1021 aatttattga tctcttcagc agttgtctcc ttttcaacga caaaaaccac atcgacaacg
     1081 cttacattcg gtgtaggaac gcggatggaa ataccgtcaa gcttaccttt cagttcagga
     1141 atgacaagcc ctatcgcttt ggctgcaccg gttgttgtcg gtatcatgct gagcgcgcct
     1201 gctctcgctc ttctgaggtc tttgtgcgga aggtcaagaa ttctctggtc gttcgtgtat
     1261 gagtggattg tggtcatgaa accgtgcttg atcttaaaat gcttgtttat tacatgggtg
     1321 atgggagcaa ggcagttcgt ggtacacgat gcgtttgaaa tgacattgtg cttttgcttg
     1381 tcataaagag aatcgttgac accgagaaca actgtaagat cttctccttt agccggagcg
     1441 gaaatgagaa ctttctttgc gcctgcctga atatgaaggt ccgcttttgc tctttcggtg
     1501 aaaagtcctg tacactcaag aactgcatct acattaagct ctttccatgg aagttttgaa
     1561 ggatctttct ctgcgattat catgatctct ttgccgtcaa cagaaataga tgcctcgcct
     1621 gctttaactt cgtgatccca aactccgtga acggaatcat atttcagaag atgtgcaagg
     1681 gttttcggat ctgtgagatc gttgatagca actatctcga tcttctcgct tttgtcagca
     1741 attctgaaaa cagctctccc gatacgcccg aaaccattga tagccacgcg aactttggtc
     1801 atgaatgcct ccttcaaaaa aaattagtcg tttatatcct tatttaaatc agaaccttgc
     1861 tctcttttat aaaaagccct ctgcatcata tatatctgct ttgtccagtt tccttccatc
     1921 tctcctgaaa aacctatatt aacagcatca agattcgcat caagattgtc acacattgaa
     1981 actataattg cttccatcgt tttcggtttt tttggtgaac cgaactcata ctcaccatga
     2041 tggctgagca ttatgtgaag aagctcaacc ttaagatcgc tgtcatcagg acagcaggtt
     2101 gaaagaagct tctcaaccca tgtcgctccc atgtagatgt ggccgagaag cttgcctctg
     2161 tctgaatatc tttcaactag agcggtcgct gtattgagct gaaactcaaa aaccttgcct
     2221 atgtcgtgta tcagtgaagc aagaacgacc agagaaacat cgaccggata tcttttttca
     2281 tatttggccg ctatgtcaaa agccatttcc gcaacattca tcgaatgttc ccacagtcct
     2341 cttctgtagg cgtggtgcat acttcttccg gcaggaattg ccgtaaaaag ctcccatacc
     2401 ttcccgctat tctcaaagtt ctcaattacc tttttatgat catccttaag cttggaacag
     2461 gtgttttccc atctctcgct aagctcagag gccgagaaca cagattccgg aagaatgcct
     2521 ctgcactctg aatcgatctt ttcaggcgga acagtttcag cgtcagttat cacaacctga
     2581 gtattcgtct gatactttgt cactttgcct gtcagtattt ttacaacatt tcccggctga
     2641 agcgccgctt cagtttcagg attgatatcc cataccttgc cgttgacctg attaattccg
     2701 tcaaaaagct cgaggtccat ataacgctgg ctgttctttc ctgtttttac agccgatttt
     2761 cttacaagaa aataaagatt tgaatcagga attattatat caccttcatc aagttcagtc
     2821 accataactc tttttcttac cattttacca ccaataacaa taaacaaata aatagaattc
     2881 agacatttta ccctgccggg caagcataat cgttgcttcg tgtgagaaga tatactttca
     2941 tgcagtaaaa atcaatcaaa ttttatccgt taaaagattt cttgagaaaa atttgacttt
     3001 tgctccattt aaaacaaact gaaaactgat ttcgtttttt aagtttccaa agtttctgga
     3061 ggtgatttga tgagtgatgt gcttaaagaa caggtactca atgaagcaaa aaagcttttc
     3121 ttcgtttacg gttttaaaaa ggtgagcatg gatgaaattg cagctgcatg caaaatgagc
     3181 aagaaaacaa tttaccagct atattcatcc aaagatgagc ttatccgcga atgtgtcgac
     3241 agaatatcaa cggaaaaagt gtcggccata cacaggatac ttgattcaga gccgctcgtg
     3301 tcaggtgagc taaacggaat gtttgagata ttcggcacta taaatcagga tgtgtcggag
     3361 gcgatgatgc gtgacatgag ctattttcct gatgtctgga acatgatcga ggaaaaaagg
     3421 atgaccgtct ttgcacgaat gggtgatatc atcgaacagg gaaaactgga cggaagcatc
     3481 aatccttctt tgaatgttga tctttttctc cgtattttca tgggaattct gcaaagattt
     3541 gccaaccccg caatgtttgc agaactgaat ttgcgtcctt ccgaattcat tgaacagatg
     3601 aaaatggtcg tttttgaagg gatactgact gttaaggcaa ggagacagta aaatgaaaaa
     3661 atatttagta attccaataa tttttatttt tgcagtattt tcttcatgcc aaaaggtcga
     3721 acccactgtg ctcacccttt ccggaacggt tgaatcaaaa gatgtgagga tctcttcact
     3781 tgtgggcggc agaataactg aggtcaatta tgacgaggga gatgaagtaa aaaaggattc
     3841 catcattgca aagatcgact gccgtgacta cgaacttcag cttgctcagg cggaaacagt
     3901 cataagcgga gcaaaggcaa agctccggct tattaaaagc ggtgcaagaa aagaggatat
     3961 cgcttcagct tccgaatcaa ataaacaggc acagattgca cttgataagg caaataagga
     4021 atatggaagg tttgaaaaac tgtttgaaac gggaagtatc actgaaaaag agcttgatga
     4081 cataaaagtt gtccgcgaca gggcacagtc acagtttgac cagagccgcc atattctcga
     4141 aaagcttcaa aacggtgcac agatagagga gatcgattca gttatagcat ctgtaaaaca
     4201 ggcagaggca tcaaaagcca ttcttttaaa aaagatctct gactgcgtta tcctttcacc
     4261 ttctgatgga actgtcctac accggcttgc agaaccgggt gaaattgcaa gccccggaat
     4321 ggcaatattg actttaagcg atataagcaa agtaagaatc agagcgtttg tacctgaaaa
     4381 agagctcggt cacataaaaa acggagccga agtgcaacta ttcattgata cattcccgaa
     4441 aatcagtttt tcaggaaaga tcgcctcaat atcaactgaa gcggaattca cgccgaaaac
     4501 tattcagacc gcagatgaac gggtaaaaac tgtttatgag ttcaaggtga tcgctgaaaa
     4561 caaagaccgt atcttcaagc ccggaatgcc tgttgacatc gtaattgata aaactgaggc
     4621 ggtattttga gtactgttct tgaaataaag aatgcttcat ttctttacgg aatgaccaaa
     4681 gcgcttgacg atgtctcttt tgaagtaaaa aaaggtgaaa ctttcggcat gatcggccct
     4741 gacggagcag ggaaaacaac tctcatacgg cttttctgcg gacttctgaa accgtcatcc
     4801 ggaaactgca cagtgcttgg atttgatact gtaaaagaaa aatatcagct tattccgaaa
     4861 atcggctatc tttcgcagaa attttcgctt tacaccgaca tgaccgtttc tgagaacata
     4921 aacttttttg cagatatcca taaagtaaga aattattcgg ctaaaaaaaa tgaactgctt
     4981 gaattcacag gtctttcaaa tttcaaagac cggctggcag gagcactgtc aggcggaatg
     5041 aaacagaaac tggcgctatc ctgcactctg atccatactc ctgagataat ctttcttgat
     5101 gagccgacaa acggggttga ccctgtcgca agaagggatt tctggaaaat attgaaagac
     5161 atctcaaaac aaggcgtttc gataattatt tcgacccctt acatcgatga agccgagaaa
     5221 tgcgaccgtg tcgctttcat gaacaggggc tctctgatcg cctgtgacac tccggcaggc
     5281 gttaaaagca gataccgtta caaacttttt gaaatagtct cacttgatgt gcaaaacaca
     5341 aaaactctcc ttgaaaaatg cgacaggata agcacagtac agattttcgg caatactctc
     5401 catgcaggaa ctgaaagaag tgaagacgac tctttcataa aagatctggc tggcaaagtt
     5461 gagggcgtta tgcggataaa agagataaag ccgtcacttg aagatgtttt cattgatctt
     5521 cttgaaaaca ggaactcgat atgaacggaa atgaattcgc tattgaagtt acaggtctga
     5581 aaaagaattt cggatctttt caggcggtaa aaaaagtttc tttcaatgta aaaaaagggg
     5641 agatctacgg atttctcgga gctaacggcg ccggaaaatc tacaacaata aaaatgcttt
     5701 gcggccttct tgagcctgat ggtggcaaag ctgttgttgc cggaactgat gtggagcatg
     5761 atcctgaagg tgtcaaaaaa aggatcgggt atatgtgtca gaaattttct ctttatgaag
     5821 aactttcggt cagtgaaaat ctcttttttt tcggcggaat atacggtctt gaaaagatcc
     5881 atcttaaaaa aaggattgag gaaattgctc aaattcttga tctttcggcg gttatcaatg
     5941 ttccttcagg agaacttcca aaaggttttc agcagcggct tgccctcgga tgtgcaataa
     6001 ttcacgagcc ggaaataata ttccttgatg aaccgacagc cggagtagac ccaatccaga
     6061 gaaggaattt ctggaatatt attaatgact tatctgttaa cggaacaact gtttttgtca
     6121 ccactcactt tcttgatgaa gcggagttct gccacagaat atcattcatt gtcgaaggaa
     6181 gtatcgttgc agatgatacg ccatcggggc ttaaagagat gttgaaaagg gaaaagtctg
     6241 aatttaacag ggaacctaca cttgaagatg ttttcatttt tcttgcgggc ggtgataaat
     6301 gagcgtaaag ccagttgtaa agaaggaatt catacagata gcaagagaca cccgcaccct
     6361 tttcatgctt gctttctttc cggcaactct tcttcttgtt ttcggatatg tcctttcatt
     6421 tgatgtaaaa gagataaaac tcggcatatt tgataatgac aggagtgaag agtcatcaac
     6481 gcttatatcg gcagtttctt caactgaata tttcattatc ggcagagtgt ttgagaacag
     6541 aagcagaata gactctttcc ttgacgatgg aactcttgac gctgtcatta taatccctga
     6601 agatttcgga aagaacattt catcaggaaa agggtcaccc gttcaggtga tgatagacgg
     6661 ttctgatggt agaagtgcag gcatcgttca ggggtatatt gccggattta ttaacagtta
     6721 ttcccagaag gtcgtcagca gttacatttc aggagcaggc agaaaagttg aaccgccggt
     6781 gaatattgag ccgcggatct ggtacaaccc tgaacttaaa tcaagtatct ttcttgttgc
     6841 cggtctgatc gttttcattc ttatgataac aggtgtgatc tcgacagcac tttctgttgt
     6901 cagggaaagg gaaaaaggga caatggaaca acttcttgta tctccgcttt ccgccacagc
     6961 cgtgataatc ggcaaaacca ttccgtatct tgtaatctcg cttttatcga ccgtcatcat
     7021 ccttgttact ggttatcttg cgttcggtgt cgaagtgaag ggctcatatc tcatgctttt
     7081 tgtttcatca actcttttca tcatgtcagc gcttgcacag ggaattctca tcagcactat
     7141 aacgacttct caacaggttg cgttctttgc cgcatctctt tcgagcttac ttccggcact
     7201 tctcctttcg ggattcattt ttcctatcgc cggaatgcct tatataattc aagcaattac
     7261 gacaatagtt cctgcaaaat attttgtgaa tctgctccgc ggaataataa tgagaggttc
     7321 cggcttcgac acgggtctga gcgatcttgc ggcactgacg atcttttcgt ttgttctgct
     7381 cacgatctcg gtaatccggc ttaaaaaggt taaactggtg taaatatgct taagttcatg
     7441 atctggaaag agtttcttca gcttaaaaga gataagaaaa tgttcggcat cattttcatg
     7501 gctccagtga tccagcttat cgttcttgga tatgcggcaa atttcgatgt ggaaagaata
     7561 tctactgcag tgtgtgatta tgacagatct aatgaaagtg cgcagtatct tgaccgctat
     7621 ttttcaggcg gctactttga aagaactatt aatctcaata aaagcggcga catagacaaa
     7681 ctacttgaaa aaggtgaaat tcaggcggcg atagttattc catccggatt cggtcgtaat
     7741 attgcaaaag gaactcaaac tgatgttcag cttataatta acggaagcga cacgaattat
     7801 gccacgaacg ctttcaatta tgccgctcag attaccgcac gattcagcac aaaaatactt
     7861 cttgaaaaaa tggctatggc cggaatgaac tctattcctc aggttaaact tgaacagcgg
     7921 cttttgtaca atcctgaact gaaaacaaaa aatttcatgg tccctgcgat aatggggctg
     7981 atactgatga taataactgt aatgctcact tcgatggcga tagtccgtga aaaagagatg
     8041 gggactattg agcagctcat tgtcacaccg ataaaaaaag ggccggttct tgcagggaag
     8101 ctcatccctt ttctgattat cggactcatt gatgtcgcac ttgtaactgc cgtcgccata
     8161 tttcttttca gaattccgtt caaagggagt ttttttcttc ttttttgcgg agctatcctg
     8221 ttccttttca acacgctcgg gctcggactt tttgtttcca ccgtttcagc cactcagcag
     8281 caggcgatga tgacagtcct gttcgttata atgatgccct tcatctatct atcgggtttt
     8341 gtcttccccg ttgagaatat gcc
//
LOCUS       DAOOYB010000120         8356 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_9470_length_8356_cov_9.548609, whole genome shotgun sequence.
ACCESSION   DAOOYB010000120 DAOOYB010000000
VERSION     DAOOYB010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8356
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9470_length_8356_cov_9.548609"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            56..280
                     /locus_tag="PL056_08120"
     CDS             56..280
                     /locus_tag="PL056_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47020.1"
                     /translation="MSLKEITLEKIKHQKTNLCRFCGNFSAYTTNGGNFIPRSEKPPF
                     SEKDISLSWVKAYEKYAYMPPQLGLIYKEN"
     gene            complement(293..2188)
                     /locus_tag="PL056_08125"
     CDS             complement(293..2188)
                     /locus_tag="PL056_08125"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPM47021.1"
                     /translation="MKKLLFCLAILFLFHLSADDNVAKMTMIVTADREPVYLKNSPEI
                     VTVVTAEEINRLHANSTTEVLEKVAGVFMETGTGSGFPKRGVASINGLPANYVLVLLN
                     GARLLSEHIHTGQNIDMIPPESIERIEIIKSAASAQYGSDAIGGVINIITSKKAKKTE
                     GKVYGTYGSYKTMNAGGKLTSPLGEKAVLSLFADVERSDGIPLLEPAHRVENEGYNKV
                     SLINSLILDPAKWFSAEFNGNFFSNRMQFRNERVYSRLIMPSGKAQIKFNKEWSLNSQ
                     IEYADWGNEASEETNRLVIPQLYTTWKGFNNKNLLNFGVDYRHSWFERSAIDGVKDQF
                     MFGIFVHDALKITDQWHISLSLRLDIPEDLNPVFSPKLAVMYKPLDDLKFRFAFARGF
                     HAPSVQELYEEGYGHGGTAYRFGNEDLKPEYSTAFSLSTEYSPHKTIEFFINGHLSII
                     DNFITPQYQGPWEEDPSVDVWRRENLIKAFVYGVEFSSKWTPVEWSWIKVGYSYAGNK
                     DRDTDRELRFQPGHSVNASLNFERDLTPKWQIGGFFSFLGGFDRKSWSWKPAKGADRT
                     DESGFITELKDYQMLSIGAQVTYDKTYQLSFKANNLLGQDIEHLDDALTVIDGEPALY
                     GSLRIFF"
     gene            complement(2241..4025)
                     /locus_tag="PL056_08130"
     CDS             complement(2241..4025)
                     /locus_tag="PL056_08130"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HPM47022.1"
                     /translation="MKKIFMVLLFLFAVSCDVRDSGEFLGGDDKKDDENNVKPDTNQT
                     VDDNEKPDEIEEQKPVCGDSKVEGTEVCDTGEKPCSELELGEGTATCKDDCTGWNTEN
                     CESQAECGNNTVEGDEQCDGNEKPCSEVGDFTDGTATCGTDCLWDTSSCEKAQPGIVD
                     PICGTNKGNEERNELKSFVEERVLGDSPEPEWVRLSWQSNPAISMTVSWTTRDSSADK
                     MTKSTILRVSKNEDMSDYIEINKDNSGSMIGSARTLPFSSAWRTVHTAEICGLDPDTK
                     YYYQVGGIGADSTENFSETYHFRTAPDPRLPAEDIKFTFIAMGDSRGAPDRLARTMEG
                     AMSKDPLFISFGGDFVDDGTSQKQWDDLFEACKDVLPYIPVLSVVGNHEKNAVNYYAQ
                     LALPGNERWYGMVVGNAVFVNLEDCWSGAGMAGSYGIACNGTMTLDKSIGETQKQFMN
                     TLFGANSALPWKFVVHHRPTYSETTDLTHGGSFNSDLKSLWNPVYEQYNVTMVFSGHD
                     HFYQRHKPLVGGNVVDNAAGVHYIVTAGAGASLYDVKNAGTVAVTKSAIHYVAVNIDG
                     KNIDMESVELNSDSGAVVGNIDTLNFSK"
     gene            complement(4045..5448)
                     /locus_tag="PL056_08135"
     CDS             complement(4045..5448)
                     /locus_tag="PL056_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47023.1"
                     /translation="MAKKIICCSLVILFVVGNLFADDLKEKKAWWCAGTCDMTRKKNE
                     ADHLYIYRWDEGKAGLWLTGYVQGRYTGINDDSSLPDGSTVDAFALQKARLAFNGKFA
                     SWAGLKLEFNIWDAVTHEVSPEEIYLDFPLIEYLGFRAGLIKSPIMYQRAMSGSAQLF
                     ADEAMVVSQKTTQIKGFEPKKTLKNFPSKDIGFMFYGDLFPWPHLDAMKKWPKGILKY
                     FFAYQNGYDMKKGTGRGDAAMYTLRFEANPFGAKSYNESSWDIDKPYMMFAFNWGKSI
                     DLEITDAKKDAGMIGVDGIISWQGVSVSGGWSMFTSTYPKSFEREKIDNNEPFDSSWK
                     TEGFYVQAAAFVPLAFWKIDLRRHLEFKFRYEEFDPFRPMSDNLYTEAMLEEFVPVGI
                     NTPQDRKTKVMTIGANVYFDLMGWKNKVKISFDYSIRDELESFRDLNDLNSNGEGKLK
                     NVQLRNDMWILQLQLAL"
     gene            complement(5441..6133)
                     /locus_tag="PL056_08140"
     CDS             complement(5441..6133)
                     /locus_tag="PL056_08140"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HPM47024.1"
                     /translation="MKKVITVLILAFLVFSCSKKKDAENLNKTDERGRSALHLSILEG
                     DEKFEKLINDGADINLKDKEGDTPLHYAVKFKREKPFQMLLNKKAVVNAVDNYGTTPL
                     SAAIQVKQYKMAETLIEKGADLNIPNSAGVTPLMFAANGAKKPAPEMVKFLLKNGAKV
                     NEVDLLGRSALFYAVGKIWVDVTEILVNAGADVNIKENQGRTVEYLLTGEGLMEEREK
                     IRGMINRSGKNG"
     gene            6340..7281
                     /locus_tag="PL056_08145"
     CDS             6340..7281
                     /locus_tag="PL056_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012610680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HPM47025.1"
                     /translation="MDKTELKKILNDVKNNVSKLVKGKDDILEKAICTFFSGGHLLLE
                     DVPGTGKTTLAKALAKSVDMNFNRIQFTPDLMPADITGVSIFNQNKGVFEFRKGPVFS
                     NIVLADEINRASPRTQSALLEAMGEGQVSTDSGQFILEKPFFVIATQNNVESKGTYTL
                     PESQMDRFTMKLSLGYVSINEEIQILDGLGFIRQLDELKPVVDSKKVVEAMETIERIK
                     VSDELKHFIVTVVNHTRNAQGVKLGASPRASLNLMKVAQAAAAFDGRDFVIPDDILSN
                     AVNVLAHRLILDSSGYITGISRENAIISILEKIKVPG"
     gene            complement(7351..7566)
                     /locus_tag="PL056_08150"
     CDS             complement(7351..7566)
                     /locus_tag="PL056_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47026.1"
                     /translation="MVPERNIKKWLEKGCCNKAYSEWKKIFESKSKESILKLISEKSE
                     LSTRLRSSSPFAGVLTSEERNKYFDNK"
     gene            complement(7646..8227)
                     /locus_tag="PL056_08155"
     CDS             complement(7646..8227)
                     /locus_tag="PL056_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47027.1"
                     /translation="MRRVDLEHIIRAAGEIAGVKEIIILGSQSILGQFPSLGEEGFNS
                     DNNVKIGFSSIEFNPCKSAEADVMVPENEELADEIEGAIGEFSLFHETHGCYAQAVDS
                     TTSKLPKRWKERLVAISNDNTNGIVGLCLEIHDLIVSKLFASREKDIEFFKSIIALNL
                     IKEETLMERLKEASLEKSDFDRIFRLIKRYFHE"
BASE COUNT         2363 a         1946 c         1430 g         2617 t
ORIGIN      
        1 ctttcttttt tcaacttttc tgcgtttttt tattcaacct atgggatggc tgtgaatgtc
       61 tttaaaagaa ataactttag aaaaaataaa acatcaaaaa acaaatcttt gtcgtttctg
      121 tggaaatttt tcagcataca caactaacgg agggaatttt attcctcgat cagaaaaacc
      181 gccattttca gaaaaagata tctctttatc atgggttaaa gcatacgaaa aatatgcata
      241 tatgccccca cagttgggac tgatttataa agaaaactga tgcttttaaa aatcagaaaa
      301 atattctaag cgatccgtat aatgcgggtt cgccgtcaat gactgtgaga gcgtcgtcaa
      361 ggtgttcgat gtcctgtccg aggaggttgt tcgctttgaa tgagagctga tatgttttgt
      421 cgtatgtgac ttgagctccg attgaaagca tctggtaatc cttgagttct gttatgaaac
      481 cggattcgtc agttctgtct gccccttttg ccggtttcca gctccacgat tttctgtcaa
      541 atccgccaag aaatgagaaa aatccgccga tctgccactt aggtgtaaga tctctttcga
      601 aattcaatga agcatttacc gaatgacccg gctggaatct gagttctcta tctgtatctc
      661 tatccttgtt tcctgcataa ctgtatccga ctttgatcca cgaccactct accggagtcc
      721 attttgagga gaattcaact ccgtaaacaa aagcttttat caggttttcc cttctccaga
      781 catcaactga aggatcttcc tcccacggtc cctgatactg aggagtgata aaattatcaa
      841 ttatgcttaa atgaccgttt ataaaaaatt ctatagtttt gtgaggagaa tattcagtgc
      901 ttaatgaaaa agccgtgctg tattccggct taagatcttc gttcccgaag cggtaagcag
      961 tccctccatg tccataacct tcttcataaa gctcctgaac agaaggtgca tgaaaccctc
     1021 ttgcaaatgc aaaacggaat ttcagatcat caagcggctt atacatcaca gcaagtttcg
     1081 gtgaaaaaac cggattcaga tcttcaggaa tgtcaagacg aagtgaaagc gaaatatgcc
     1141 actgatctgt aattttcaga gcatcatgca caaagatccc gaacataaac tgatccttaa
     1201 caccgtctat tgcacttctt tcaaaccagc tgtgacggta atcaactccg aaattgagaa
     1261 gattcttgtt gttaaatccc ttccatgttg tgtaaagctg aggaattaca agccggtttg
     1321 tctcttcact tgcttcattt ccccagtccg catattcaat ctgactgttc aatgaccact
     1381 ctttattgaa ttttatctgt gcttttccac ttggcatgat aagccggctg taaacacgct
     1441 cattcctgaa ttgcatcctg tttgaaaaga aattcccgtt aaattcagca ctgaaccatt
     1501 tagcagggtc gagtataagt gaattaatca gcgaaacttt gttgtacccc tcattttcaa
     1561 ctctgtgtgc cggctcaagc aagggtatcc cgtcacttct ttctacatcg gcaaaaaggg
     1621 acagcactgc tttttcacca agtggagaag tgagttttcc accagcgttc atcgtcttat
     1681 atgaaccata tgttccgtaa actttccctt cggttttttt tgcttttttc gatgtaataa
     1741 tgtttataac tccgccgatc gcatcacttc cgtactgagc agatgcggca gatttaatta
     1801 tctcaattct ttcaatgctt tccggcggaa tcatatcaat attctgacct gtgtgaatgt
     1861 gttcactcag cagccgtgca ccgtttaaaa gaacaaggac ataatttgca ggaagaccgt
     1921 taattgaggc gactcccctt ttcgggaaac cgcttcccgt ccctgtttcc atgaaaacac
     1981 cagccacttt ttcaagcacc tcagtagttg aattcgcatg aagcctatta atctcttcag
     2041 cagtgacaac agtcacaatt tcaggactgt tctttaaata gaccggctcc cggtcggcag
     2101 taactatcat cgtcattttt gcaacattgt catcggcact cagatgaaac aggaacaaaa
     2161 tggctaaaca gaaaagtaat ttcttcattt tttcaccaca caaaacaacc atcacacaaa
     2221 catcagatca taataagaaa ttatttcgaa aaattcaagg tatcaatatt cccgacaaca
     2281 gcaccggaat cagaattgag ctcgactgat tccatatcga tattctttcc gtcaatgttt
     2341 acagcaacat agtgaattgc agatttagtg accgccactg ttcctgcatt tttaacatca
     2401 taaagacttg ctcccgcccc tgctgtaaca atataatgaa cacctgccgc attatctaca
     2461 acattccctc cgacaagcgg tttgtgtctc tgataaaaat gatcgtggcc actgaacacc
     2521 attgttacat tatactgttc gtaaacaggg ttccagagag atttcagatc actgttaaag
     2581 cttccgccgt gagtaaggtc tgtcgtttcg ctgtatgtcg gtctgtgatg aacaacaaat
     2641 ttccatggaa gagctgaatt tgcaccgaaa agagtgttca tgaactgttt ctgagtttct
     2701 cctatcgatt tatcaagcgt cattgtcccg ttgcacgcaa taccgtaaga gcccgccatc
     2761 ccggctccgc tccagcaatc ctcaagattt acaaaaacag cgtttcctac aaccattcca
     2821 taccatcttt catttccagg aagtgcaagc tgtgcatagt aatttactgc attcttctca
     2881 tgatttccta cgacagaaag gacaggaata taaggaagca catctttgca tgcttcaaaa
     2941 agatcatccc actgtttctg agaagttccg tcatcaacaa aatctccgcc gaaactgatg
     3001 aaaagcggat ctttgctcat agccccttcc attgtccttg caagacggtc aggagctcct
     3061 ctggaatcgc ccattgcaat aaaagtgaac ttgatatctt ctgccggaag tctcggatcg
     3121 ggagcagttc tgaaatgata agtttcgcta aaattttcag tgctgtcggc tcctattcct
     3181 ccaacctgat aataatattt cgtgtctgga tcaagtccgc agatctcagc cgtatgaaca
     3241 gttctccatg cactgctgaa aggcagagtc cttgctgaac cgatcatact tccggaattg
     3301 tctttattta tctcgatata atcgctcata tcctcattct tactgactct cagtattgtg
     3361 gatttcgtca ttttatcagc gctgctgtcc cttgttgtcc acgaaactgt cattgagatg
     3421 gccggatttg attgccagct taaccttacc cactccggct cagggctatc tcccagaact
     3481 ctttcttcaa caaaagattt cagttcgttt ctctcttcat tgcctttatt ggttccgcaa
     3541 atcgggtcta ctatccccgg ctgtgccttt tcacaacttg atgtatccca cagacaatcc
     3601 gtcccgcagg tagccgttcc gtccgtaaag tcgcctactt ccgaacacgg tttttcattt
     3661 ccgtcacact gttcatcacc ctcaactgtg ttgtttccgc actctgcctg actttcacag
     3721 ttttcagtat tccatccggt acaatcatct ttgcaagttg ccgttccttc accaagttca
     3781 agttccgaac acggtttttc tcctgtgtca caaacttccg tcccttcaac ttttgaatca
     3841 ccgcagacag gtttctgttc ttcaatttca tcaggttttt cattatcatc aacggtctga
     3901 ttagtatccg gcttaacatt attctcatca tcttttttgt cgtcaccccc tagaaattca
     3961 ccgctgtccc ttacatcaca tgacacagca aataaaaaca gaaggaccat aaagatcttt
     4021 ttcattgtaa tctccgttaa atatttataa agcgagttgt aactgcagta tccacatatc
     4081 attccttagt tgaacatttt tcaactttcc ttctccgtta ctgttaaggt cattcagatc
     4141 cctgaaactt tcaagttcat ctctgataga gtagtcaaaa cttattttca ctttgttctt
     4201 ccaacccata agatcaaaat atacattggc gcctattgtc ataactttgg tttttctatc
     4261 ctgcggagtg tttatcccga caggaacgaa ttcttcaagc attgcttcag tataaagatt
     4321 gtcactcatc ggtctgaaag gatcgaattc ctcatatctg aatttgaact ccagatgtct
     4381 tctgagatct atcttccaga aagcaagagg aacaaatgcc gccgcctgaa cataaaaacc
     4441 ttctgttttc catgaagaat cgaacggctc attgttatcg atcttttctc tttcaaaaga
     4501 cttagggtat gtgcttgtaa acattgacca gccacctgaa acactcacac cctgccaact
     4561 gatgattccg tcaacaccta tcatcccggc atctttttta gcatccgtta tttcaaggtc
     4621 tatacttttg ccccagttga atgcgaacat catatagggt ttatcaatgt cccatgaact
     4681 ttcattgtat gacttagctc cgaaagggtt tgcctcaaaa cggagtgtgt acattgcggc
     4741 atcacccctt cctgtccctt ttttcatgtc gtatccattc tggtaggcaa aaaagtattt
     4801 caagatccct ttgggccatt ttttcattgc atcaaggtga ggccacggga aaagatcacc
     4861 gtaaaacatg aatccgatat cttttgacgg gaagttcttt aatgtctttt tcggttcaaa
     4921 accttttatc tgagttgttt tctgtgaaac taccatcgct tcatccgcaa agagctgtgc
     4981 agaacccgac attgctcttt gatacattat cggagattta atgagacctg ctctaaaacc
     5041 cagatactcg atcaacggaa aatcaagata tatctcttca ggtgaaactt catgagtaac
     5101 agcatcccat atattaaatt caagcttaag ccctgcccaa cttgcaaatt tcccattgaa
     5161 tgcaagccgt gctttctgga gagcaaacgc gtcaactgtt gaaccgtcag gaaggctgct
     5221 gtcgtcattt atccctgtgt atcttccctg aacataaccg gtaagccaga gtccggcttt
     5281 cccttcatcc cacctgtata tgtagagatg gtcagcttca ttttttttcc gtgtcatatc
     5341 acaagttccg gcacaccacc aagctttttt ctcttttaaa tcatctgcaa aaagattgcc
     5401 aactacaaaa agaataacca atgaacagca aataattttc ttagccattt tttccactcc
     5461 ggtttatcat tcctctaatc ttttccctct cttccatcaa accttcacca gtcagcagat
     5521 actcaacggt tctcccctga ttttctttaa tatttacatc ggcacctgca ttgacaagta
     5581 tctctgtgac atcgacccat atcttgccga ccgcataaaa aagtgccgac ctgcctaaca
     5641 agtcaacttc attgactttt gcaccatttt tcaagaggaa tttcaccatt tcaggagccg
     5701 gtttttttgc tccgtttgcg gcaaacataa gcggtgtcac tcccgctgaa ttgggaatat
     5761 tgagatcagc ccctttttca atgagagttt cagccatttt atactgcttg acctgaatag
     5821 cggcactcag cggagttgtt ccgtaattat cgactgcgtt gaccaccgct ttcttattta
     5881 aaagcatctg aaacggcttt tctcttttaa atttcactgc ataatgaaga ggcgtatcgc
     5941 cctctttatc cttgagatta atatctgcac cgtcattgat cagcttttca aacttttcat
     6001 ccccttcaag aatcgaaaga tgaagagcgc ttcttcccct ttcatcggtt ttattaaggt
     6061 tttcagcatc ttttttctta gagcatgaaa acaccagaaa agccagaatc agaactgtaa
     6121 ttaccttttt catttaatct ctccgcttca cacacaaaaa aacacaggtg gatagtagca
     6181 acattcggtt agtttttgat gagctcattc ttaatattca attaacaccg catttgtttt
     6241 aaccacccat gactcttctt ctctttattc tttactcctg ctcataaatt gattaaaata
     6301 ctgtgcaaat tgcttattcc aactctttcc ggaggtccca tggacaaaac cgaactgaaa
     6361 aagatactta acgatgtaaa gaataatgtt tccaagcttg taaagggcaa agatgacatt
     6421 cttgaaaaag ctatctgcac ctttttttca gggggtcatc tcctccttga ggatgttccg
     6481 ggaacgggta agacaactct ggcaaaagca cttgcaaaat ctgttgacat gaactttaac
     6541 agaatacagt tcacccctga tctcatgccg gcagacataa caggtgtttc cattttcaat
     6601 cagaacaaag gtgtttttga attcaggaaa ggtcccgttt tttcaaatat tgtccttgcc
     6661 gacgagataa accgtgcaag cccgagaact cagtcagccc ttcttgaagc tatgggcgaa
     6721 ggacaggtga gcaccgattc ggggcagttc atccttgaaa aaccgttctt tgtgatagca
     6781 actcagaaca atgttgaatc aaaagggaca tacactcttc ccgaatcaca gatggaccgt
     6841 ttcacaatga aactttctct tggatatgta tcaattaatg aggaaatcca gatacttgac
     6901 ggattagggt tcatcagaca gcttgacgag cttaagccag ttgtagattc taaaaaagtt
     6961 gttgaagcaa tggaaacaat cgaaagaata aaggtgagtg acgagctcaa acacttcatc
     7021 gtgaccgttg taaatcatac cagaaatgct cagggagtta aactcggagc aagccccagg
     7081 gcctcactta acctcatgaa ggttgctcag gcggccgccg catttgacgg tcgagatttt
     7141 gttatccctg atgacatctt aagtaatgcc gtcaatgttc tcgcccaccg tctaattctt
     7201 gacagctccg gctatattac cggaatctca agagaaaacg caataatatc aatattagaa
     7261 aaaataaaag ttccgggtta gaatgcaaag ataaaatact tcgaaaataa ctcaaatttt
     7321 caaaaaaaag ttccgttgat cagattgcga ctatttattg tcaaaatatt tgtttctttc
     7381 ttctgaagtg agaacccctg caaaaggaga ggaagatctt aatctggttg aaagctctga
     7441 tttttcagat atcaatttca aaattgactc cttcgacttt gattcaaata ttttcttcca
     7501 ttctgaatat gccttattgc aacacccttt ttcaagccat ttcttaatgt ttctttcagg
     7561 aaccacccac atctcaggat tttttgacag tttttgtgct acttccagat gaatcatcag
     7621 agatcttatg tcagaacgta actgtctatt catgaaaata cctttttatt aatctgaata
     7681 ttctatcgaa gtctgatttt tctaaagatg cttctttcaa tctttccata agagtttctt
     7741 cttttattaa atttaaggcg attattgatt tgaaaaattc aatatctttt tcacgggaag
     7801 caaaaagttt agaaacaatt agatcgtgta tttctaagca taaaccaaca attccattag
     7861 tgttatcatt agaaatagca acaagtcttt ctttccatct ttttggcagc tttgatgttg
     7921 tactatcaac agcctgtgca taacatccat gtgtttcatg aaaaagtgaa aattctccaa
     7981 tagctccttc aatttcgtct gctaattcct cattttccgg aaccataaca tctgcttcgg
     8041 cagatttaca aggattaaat tctatagatg aaaaaccaat tttaacattg ttgtcactgt
     8101 taaatccttc ttctcccaaa gaaggaaact gacctagaat agattggctt ccaagaataa
     8161 ttatctcttt tacacccgca atttctccag ctgcccggat tatgtgttcc aaatcaaccc
     8221 ttctcatatt tacatcccat cagtaaataa cagtaattca ttatatttac aaagaatctt
     8281 ctgtcaaaca gtttaataat tgtttaataa attgaacaga ttttctccct caacctgaaa
     8341 ctgtccgctt tttcaa
//
LOCUS       DAOOYB010000121         8306 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_9533_length_8306_cov_10.849352, whole genome shotgun sequence.
ACCESSION   DAOOYB010000121 DAOOYB010000000
VERSION     DAOOYB010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8306
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9533_length_8306_cov_10.849352"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            47..1045
                     /gene="hgcA"
                     /locus_tag="PL056_08160"
     CDS             47..1045
                     /gene="hgcA"
                     /locus_tag="PL056_08160"
                     /inference="COORDINATES: protein motif:HMM:NF040863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042192 - methylmercury biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercury methylation corrinoid protein HgcA"
                     /protein_id="HPM47028.1"
                     /translation="MQINTIRQPYVSGFVNSGNILIPKIFTTLSLKDRFSGFMVRLGY
                     RRMHYTVRQGLYAVGNPDKNSPVIVTANYKLTFDVVRNAISGMNLWMLVLNTHGVNVW
                     CAAGKGTFGTGEIARMVKMTKLSTVVSHRNLILPQLGAPGVSAHRVKKLTDFSVIYGP
                     VKISDLKKFIDNGNVADRSMRTVEFPLIERMKVAPLELVLGWKILIGVLILSLLNISS
                     FIPLIGSVIAGIFLFPALLPYLPFRSFTLKGAILGLVWACFCSLFIATDIFTVLEFLL
                     IIPPVVAYMAFNFTGSTTFTSPSGVRYEIKKYTWPFALWMSAGIIMYFVNVFRGLL"
     gene            1042..1305
                     /gene="hgcB"
                     /locus_tag="PL056_08165"
     CDS             1042..1305
                     /gene="hgcB"
                     /locus_tag="PL056_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014826631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042192 - methylmercury biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercury methylation ferredoxin HgcB"
                     /protein_id="HPM47029.1"
                     /translation="MKYIDNVVSLCFDESKCTGCGMCAEVCPHNVFKVDRIAEIINKN
                     NCIECGACAKNCPFQAIKVCSGVGCGYAMIKSKITGKEESCCC"
     gene            1319..1672
                     /locus_tag="PL056_08170"
     CDS             1319..1672
                     /locus_tag="PL056_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015837170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HPM47030.1"
                     /translation="MKDLAQFFKALSDETRLQIVALLLMKEELCVCDIENTLGMTQSK
                     ASRHLRYLMNSGILTDTRKNIWVYYSISKSLSSNELAVLESLKKCLGSDVRNELKKKL
                     DGWMKSKEKSNVCKL"
     gene            1684..2739
                     /gene="arsB"
                     /locus_tag="PL056_08175"
     CDS             1684..2739
                     /gene="arsB"
                     /locus_tag="PL056_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015009206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HPM47031.1"
                     /translation="MEKNVGGISFFEKYLSLWVALCMVAGVMIGKFAPAIPQFLGKFE
                     YAKVSIPIAILIWLMIYPMMMKVDFASVKDVGKNPKGLFVTWITNWLIKPFTMYAIAW
                     FFFFIVFKSLIPTDLARDYLAGAILLGAAPCTAMVFVWSHLTKGNPAYTVVQVATNDL
                     IILVAFTPIVALLLGISGITIPWNTLFLSVVLFVVIPLAGGILTRKTVIKNKGLEYFN
                     TRFITKFNNITIGGLLLTLVIIFSFQGHVILENPLHILLIAIPLTIQTFFIFFVAYLS
                     SKKLCLRHSIAAPAGMIGASNFFELSVAVAIALFGPTSPVALATIVGVLVEVPVMLTL
                     VKIANKTKHWFPKEEKS"
     gene            2736..3164
                     /locus_tag="PL056_08180"
     CDS             2736..3164
                     /locus_tag="PL056_08180"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HPM47032.1"
                     /translation="MKKNVIFICIHNSARSQMAEAYLKHFASDRFNVWSAGLEPGKLN
                     PVVVKAMALDGIDISGHTTKSVDEFIDGHIKFDFVVTVCDETAAEACPFFPGQGERHH
                     WGFQDPPGLTGTDEEKLARTIQIRDQIKEKVKGFAGSKLS"
     gene            3225..3464
                     /locus_tag="PL056_08185"
     CDS             3225..3464
                     /locus_tag="PL056_08185"
                     /inference="COORDINATES: protein motif:HMM:NF024064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HPM47033.1"
                     /translation="MTNRMTISMPDEYVNEIDEIQKELKVNRSTLLRMAFETFIKEHR
                     RSKLRKRAALMREEYLSNSELNSMKDLDGDDFETI"
     gene            3451..3795
                     /locus_tag="PL056_08190"
     CDS             3451..3795
                     /locus_tag="PL056_08190"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015117297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HPM47034.1"
                     /translation="MKQYEIWLINLDPAIGAEIKKTRPCVVINNNDIGILPLKIVTPL
                     TDHKPHYDKNPWMLTVNPDKFNNLEKKSVIDLFQLRSISEIRFIRKLGELNPKIAASI
                     PQTLEMIFEPEM"
     gene            3987..5396
                     /locus_tag="PL056_08195"
     CDS             3987..5396
                     /locus_tag="PL056_08195"
                     /inference="COORDINATES: protein motif:HMM:NF013776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM47035.1"
                     /translation="MFIGRKRELRLLEESYALDRGSIIVMYGRRRIGKSTLIQEFIKN
                     KEHTFYFEGIESGNTKAQILHFTSALRKMVNDPVLDSVMFSNWDQVFTYFTDRVVNSL
                     PEKKLILVLDEIQWLAAGRKTLIAMIKYYWDNFWKGKNIKLILCGSIASFMVENIIKS
                     NALYGRIDLEILLKGLKPSESLSFFSERKSKDEILKYLLVMGSVPKYLEFINQKKSFN
                     KNINDLLFSKTGRMNNEIEKIFYSQFRESSVYRNIVNLLQKSVLGLYEISKLLNMQSG
                     GGLKRYLENLENAEIIVSFIPYNKKSGTKIKKYALSDEYLRFYFKYIEPNKEIISQSD
                     SEILFETLTKNSFEKWMGIQFERFCLKNKNLLAEKMGFKNQVLIAAPFYEKGDTSFQI
                     DLLFVRSDNVITICEIKYWNSEVGTNIISEMKRKTELLKNTKGYSIETALITTFGADK
                     SLKETNYFDHILTMDDIFS"
     gene            complement(5514..7709)
                     /locus_tag="PL056_08200"
     CDS             complement(5514..7709)
                     /locus_tag="PL056_08200"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HPM47036.1"
                     /translation="MTNNNPEILAPAGSLASAVYAFQGGADAVYLGLSDFSARKFATN
                     FTFDHLRRLKKQALLLNKKIFIAVNTVIREEELDDLFKYLLNLSFFEPDAIIIQDIGM
                     IQIVKKYFPDIPLHASTQMSVHNTEGVKFLQKLGISRVVLSRELTFNELTVIRKNCPD
                     MELEVFIHGALCYSFSGKCLASGFIANRSANKGECIQLCRDYYTEDNGSSKFHFSCRD
                     LALYESVRKYADIGIDSVKIEGRMKPPEYSFYASKLYRAILDGADENEIAELKQNLAL
                     VFERFTTNSFFASTSGDELICNSYSSHKGIELGYVEKTAKESFFINLRHDLSIKDGLM
                     FFEREGDIEPHKFSVSQIKTVSAQDTKWLESGNIAEVFSETVPKAGTLIFQISHRNLD
                     MKNINDLALPQYFVKTDVDAVINDDSIKISGKMKHGITAAIEEKIVVEEAKKESDINA
                     IMQKHLKTADDGNLFEIDSINVINQSRFDKNRIFIPPSQLKSAKRAFVSALSTAFDNK
                     IVGFSAPEIPQKEINSQHEALLSIISDREKINPVTFIEGKESRELPFALNFHLKNIDT
                     LHRFENFVFVPLKPVIKDGDSYMSEFIELVNSRSDITFVAGLNNISHFSLIKHNFKNV
                     LFFCDFFIYAANRFALDFINSLSNGNIISVFKWIEDSSVSLLSSGIPVLEISQNFKRP
                     LFYALGCYKKHNLKTDCGSCTKEGVCLLKNRGRNYKVIVYDCVTYMFLE"
     gene            complement(7714..>8306)
                     /locus_tag="PL056_08205"
     CDS             complement(7714..>8306)
                     /locus_tag="PL056_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47037.1"
                     /translation="SSGRIFLQFYDDDAKNDSSTGQKPIVWSDDNFATFTGKSNSFDI
                     TVPSGTFSMESVMDMDGGAVDEIWIAGFCGSGFNFKPCTIDTTDGGQTWWERLVIGAG
                     KLGPISVLDSNHVYIASSAKAMYKWGDPNEDMTETEVPDETVTPDESIENSDENETVD
                     NNQPIDENTTTDTETVSDDEELIDEEEVGCGCSFIR"
BASE COUNT         2610 a         1634 c         1602 g         2460 t
ORIGIN      
        1 tgcataatcg tttaagcggt tatgttgttt tggaggatag aaagcggtgc agataaatac
       61 aatcagacag ccgtatgttt caggatttgt caattcggga aacatattga taccaaaaat
      121 attcactact ctttccttaa aagaccgttt ttcaggattc atggtgcggc ttggatatag
      181 aagaatgcat tacactgtca ggcagggact ttatgctgtg ggaaatcctg ataaaaactc
      241 gccagttatc gtgacagcga actataagct cacttttgat gttgtaagaa atgcaatatc
      301 aggaatgaac ctgtggatgc ttgttcttaa cactcacggc gtaaatgtct ggtgtgcggc
      361 aggcaaaggg acattcggga caggcgagat cgcaagaatg gtgaaaatga caaagctttc
      421 aactgtggtg tcacatagaa accttattct gcctcaactt ggagctccgg gagtttctgc
      481 ccacagagtg aagaaactga cagatttttc ggttatttac ggacctgtga aaatatcaga
      541 tcttaaaaaa ttcattgata acggcaatgt tgcagacaga tcaatgagaa ctgttgagtt
      601 tccacttatt gaaaggatga aagtggcacc gcttgagctt gtgctcgggt ggaaaatact
      661 gatcggagtg ctcattcttt ctttgcttaa catttcgagc ttcatacctc ttatcgggag
      721 tgtaattgcc ggaatatttc tcttccctgc ccttcttccc taccttccgt tcagatcatt
      781 tacactgaaa ggtgcaattc ttggactcgt atgggcttgt ttctgctcac tttttattgc
      841 tactgacata ttcacagtcc ttgagttcct actcataatc ccccctgtcg tggcatacat
      901 ggctttcaat tttaccggct caacgacttt cacttctcca tccggcgtca gatacgaaat
      961 aaaaaagtac acctggcctt ttgctctgtg gatgtcggca ggaataatta tgtattttgt
     1021 taatgttttt cgaggtctgt tatgaaatat attgataatg tagtttcgct ttgttttgac
     1081 gagtcaaaat gtaccggctg cggaatgtgc gctgaagtgt gtccgcacaa tgtgttcaaa
     1141 gttgacagaa tcgctgaaat aattaataaa aacaactgta tagaatgcgg agcatgtgca
     1201 aaaaactgcc cttttcaggc aataaaagtc tgttcgggtg tagggtgcgg atatgcgatg
     1261 ataaaatcaa agatcacagg taaagaagaa tcgtgctgtt gctgataagg agggaaaaat
     1321 gaaagatctt gctcaatttt ttaaggcgct ttcggatgaa accagattgc agatcgttgc
     1381 actcttactg atgaaagagg aactttgtgt gtgcgacatt gaaaatacgc tcgggatgac
     1441 tcagtcaaag gcatcgcgac acttgagata tttaatgaat tcaggcattt tgactgatac
     1501 tcgcaaaaac atctgggtct attattctat cagcaagagt ctgagttcaa atgagcttgc
     1561 tgttcttgag agcttaaaaa aatgtctggg gagcgatgtc agaaacgagt tgaagaaaaa
     1621 gctcgacggg tggatgaaaa gcaaagaaaa atcaaatgtt tgtaaattat aacggagaaa
     1681 actatggaaa aaaatgttgg cggtatcagt ttttttgaga aatatctctc gttgtgggtg
     1741 gctctctgta tggttgcagg agtaatgatc ggaaagtttg cccctgcaat accgcagttc
     1801 cttggaaagt ttgaatatgc taaagtctca attccgatcg ctattctgat ctggctcatg
     1861 atctatccga tgatgatgaa agttgatttt gcaagtgtta aagatgtggg aaaaaatccg
     1921 aaaggacttt ttgtgacctg gatcaccaac tggctgataa agccgttcac gatgtatgca
     1981 atagcgtggt ttttcttttt tatcgtgttt aaatcgctca tcccgactga ccttgcaaga
     2041 gattatcttg ccggagcgat acttctcggg gcggctccct gcactgcgat ggtttttgtc
     2101 tggagtcatc tcacaaaagg gaatcctgca tataccgtcg ttcaggtcgc aacaaacgat
     2161 ctcatcatcc ttgtcgcttt cactccgatc gttgcactcc ttcttggaat cagcggaata
     2221 acaattccct ggaacaccct ttttctatct gtcgtcctct ttgtcgtgat cccgcttgca
     2281 ggtggaattc tgacaagaaa aacagttata aaaaacaaag ggctggaata ttttaacacc
     2341 agattcatca ctaaattcaa taacataaca atcggagggc tgctgctcac ccttgtcata
     2401 atattttcat ttcaaggtca tgtcatcctt gaaaatccgc ttcacatact gcttattgca
     2461 attccgctca caattcagac attcttcatc ttttttgttg catatctcag ttctaaaaag
     2521 ctttgtctgc gtcacagtat cgctgctcct gccggaatga taggagcatc aaatttcttt
     2581 gaactttccg ttgctgtcgc cattgcactt tttggaccga cttcacccgt tgcactcgct
     2641 acaattgtgg gagttcttgt tgaagtccct gtaatgctta cactcgtaaa aattgcaaat
     2701 aaaacaaaac actggttccc caaggaggag aaatcatgaa aaaaaatgta atattcatct
     2761 gcatccacaa ctctgcaaga agccagatgg cagaagcata tctgaaacat ttcgcatcag
     2821 acagattcaa tgtgtggagt gccggacttg aaccgggaaa gcttaatcca gttgttgtaa
     2881 aagcgatggc acttgacgga atagacatat caggacacac cacaaaatca gttgatgaat
     2941 tcatagacgg acacataaaa ttcgatttcg ttgtcacagt ctgcgatgaa accgccgcag
     3001 aagcatgccc cttctttccc ggacagggcg agcgacacca ctggggattt caagatccgc
     3061 caggactcac cggaaccgac gaagaaaaac ttgcccgcac catccagatc agagatcaga
     3121 taaaagagaa agttaagggt tttgcgggat caaagctctc ttgacacacc tttattcaca
     3181 agtatagtat gaattgtgtt cataattatt ttatgaggtt tgttatgacg aatcgtatga
     3241 cgatttcaat gccggatgaa tatgtaaatg agattgatga gatccagaaa gagcttaagg
     3301 tgaaccgctc aactctttta aggatggcat ttgaaacatt catcaaagaa catcgaagat
     3361 caaaattgcg taaaagggct gctttaatga gggaagaata tctctcaaac agtgaactta
     3421 attcaatgaa agatcttgat ggagatgact ttgaaacaat atgaaatatg gcttatcaat
     3481 cttgatccgg caatcggggc tgaaataaaa aaaacaagac cttgcgttgt cataaacaat
     3541 aatgacatcg gcatcttacc tcttaaaatc gttactcccc ttaccgatca caaaccacat
     3601 tatgacaaga atccatggat gctgacagta aaccccgaca aattcaacaa tcttgaaaaa
     3661 aaatctgtca ttgatctttt tcagcttcga tcaatatccg aaataagatt catcagaaaa
     3721 ctcggtgaac tcaaccccaa aattgcagca tccatccccc aaacccttga aatgatcttt
     3781 gaaccggaaa tgtgaatttg caaactttcc cgcaccattc agatcagaga tcagataaaa
     3841 gagaaagtta agagttttgt tttctgaaac ttgacaaatc cgcacaaagt tataacatct
     3901 aattgtgtgc ggatacacta tttgtttttg tggctaatcc gcacaaactt atgctcacca
     3961 attgtgtgcg gattaggagg tgtgccgtgt ttataggaag aaaacgggaa cttagattgc
     4021 ttgaagaatc gtatgctttg gatagaggat caataattgt aatgtatgga agaaggcgaa
     4081 taggaaaaag cacactgatt caagagttta taaaaaacaa agaacacaca ttttattttg
     4141 agggtattga atcagggaat acaaaagctc agatactcca ttttacatct gctttaagaa
     4201 aaatggtaaa cgatcctgtc cttgacagtg tgatgttttc gaattgggat caagttttca
     4261 catattttac cgacagagtt gtaaattcac ttcctgaaaa aaagctcata cttgttcttg
     4321 atgaaatcca gtggcttgcg gcaggcagga aaacattgat cgcaatgata aaatattact
     4381 gggataattt ctggaaagga aaaaatatta agctgatact ttgcggttct atagcttctt
     4441 ttatggttga aaatatcata aaatctaatg cactttatgg cagaattgat cttgaaatcc
     4501 ttctaaaagg actcaaacca tctgaatcct tgagcttttt ctcagaaaga aaaagcaagg
     4561 acgaaattct gaaatatttg cttgtaatgg gaagtgtccc caaatacctc gaatttatta
     4621 accagaaaaa atcattcaat aaaaatataa atgacttgct tttctcaaaa accggcagaa
     4681 tgaacaacga aattgaaaag atattctaca gccagtttag agaatcatct gtctatagaa
     4741 acattgttaa tttattgcag aaaagtgttt tgggacttta tgaaataagt aagttgctga
     4801 atatgcagtc cggaggcggt ttgaaaagat atctggaaaa tctggaaaat gctgaaatca
     4861 tagtttcatt tattccttac aataaaaagt ccgggacgaa aatcaaaaaa tatgccctgt
     4921 cggatgaata tcttcgcttt tactttaaat atattgagcc gaataaagag atcatatctc
     4981 aaagcgattc agaaattctt tttgagacac tcacaaaaaa cagttttgaa aaatggatgg
     5041 ggattcagtt tgaaagattc tgcctgaaaa acaaaaatct tctggctgaa aaaatgggat
     5101 ttaagaatca agtgcttatt gctgctccgt tttatgaaaa aggtgatacc tcttttcaaa
     5161 tagaccttct cttcgtaagg tctgataatg tcattacaat ttgtgaaata aaatattgga
     5221 acagcgaagt cggaacaaat attatttcag aaatgaaacg gaaaactgag ctccttaaaa
     5281 acacaaaagg atactcaatc gaaacagcac tcattacaac attcggtgca gataaatctt
     5341 taaaagaaac aaattatttc gatcatattc tgacaatgga cgatattttt tcctgaaacc
     5401 tgccccgcac cattcagatt agagatcaga taaaagagaa agttaaggag tttgtagatt
     5461 aaatcctcga aatgatcttt gaaccggaaa tgtgaaatga tattaaattc ttatcactcc
     5521 aaaaacatat aagttacgca gtcatacaca atgactttat aatttcttcc tctgttttta
     5581 aggagacaaa ccccttcttt tgtgcagctg ccacaatcag ttttgagatt gtgttttttg
     5641 taacagccga gagcataaaa aagaggtctt ttgaaatttt gcgatatttc aaggaccggt
     5701 attcctgatg ataaaagcga aactgagctg tcttcgatcc atttaaaaac cgatatgata
     5761 ttcccgtttg acaatgaatt tatgaaatca agggcaaatc tgttcgccgc atagatgaaa
     5821 aaatcacaga aaaaaaggac atttttaaag ttgtgcttaa taagtgaaaa atgagaaata
     5881 ttgttaagtc ctgcaacaaa tgttatatca gaccttgaat taacaagttc aataaattca
     5941 gacatatagc tatcaccatc ctttatgacc ggctttaacg gaacaaaaac aaaattttca
     6001 aaacggtgaa gtgtatcaat gtttttaaga tgaaaattca gtgcaaaagg aagttctctg
     6061 ctctctttcc cttcaatgaa agtaacaggg tttatctttt cacggtcaga aattattgaa
     6121 agaagtgctt catgctgaga attgatctct ttctgaggaa tttcaggtgc agaaaaacca
     6181 acaattttat tatcaaaagc agtgcttaga gccgaaacaa aagctctttt tgccgatttc
     6241 aattgtgacg gcggaataaa tatccggttt ttatcaaaac ggctctgatt gataacattt
     6301 atagaatcta tctcaaaaag attcccatca tcggcagttt taagatgttt ctgcatgatc
     6361 gcatttatat ctgattcctt ttttgcctct tctactacga ttttctcttc aatagctgca
     6421 gttattccgt gcttcatctt tccagaaatc ttaattgaat catcatttat taccgcatca
     6481 acatcggttt tcacaaaata ttgcggcaaa gcaagatcgt tgatgttctt catatcaagg
     6541 tttctgtgac ttatctgaaa tatcaaagtc cccgcctttg ggacagtttc tgaaaaaacc
     6601 tctgcaatat ttccggattc aagccatttt gtatcttggg ctgaaacggt ttttatctga
     6661 cttacgctga atttatgcgg ttcaatatcc ccttcccttt caaaaaacat caatccatcc
     6721 ttaattgaaa gatcatgccg gaggtttatg aaaaaactct cttttgccgt cttttccaca
     6781 taaccgagtt caatcccttt gtgcgaagag taactattgc atatcagttc atctccactt
     6841 gttgaagcga aaaaactatt cgttgtaaac cgttcaaaaa caagtgcaag attctgtttt
     6901 aactcagcaa tttcattttc atcagctcca tcaaggatcg ctctgtaaag ctttgaagca
     6961 taaaaagaat attccggcgg cttcatccgc ccttcgatct taacgctgtc aattccaata
     7021 tcggcatatt ttctcaccga ctcgtataga gcaagatcgc ggcacgaaaa atgaaatttt
     7081 gaagaaccgt tatcttcagt gtaataatcg cgacagagct gaatacattc ccctttattt
     7141 gcacttctat ttgcaatgaa accggatgcc agacacttcc ccgaaaatga ataacagagc
     7201 gcaccgtgaa tgaaaacttc caactccatg tccggacagt tctttcttat caccgtcagt
     7261 tcattaaaag tcagttccct tgacagaaca actctgctga tcccgagttt ctgaagaaat
     7321 ttcacccctt cagtgttatg caccgacatc tgagttgaag catgcagagg aatgtcggga
     7381 aaatactttt ttacgatctg tatcatcccg atatcctgaa tgattatcgc atcgggctca
     7441 aaaaatgaaa gattcagaag atatttaaaa agatcatcaa gctcctcttc acggatcaca
     7501 gtatttaccg caataaatat ctttttattt aaaagaagtg cctgtttttt aagccgcctt
     7561 aagtggtcaa aagtaaaatt tgtcgcaaat ttacgggctg aaaaatcgct caaacccaga
     7621 tatacagcat cggcaccgcc ttgaaaagca tatacggctg aagccagaga accggcagga
     7681 gcaagaattt cgggattgtt atttgtcaag agatcacctt atgaacgaac agccgcatcc
     7741 gacttcttct tcatcaatta attcttcatc gtcactcact gtctcggtat cggtcgttgt
     7801 gttctcgtca attggctgat tgttgtcaac tgtctcattt tcatcagaat tttctattga
     7861 ttcatcgggc gttacagttt catcaggaac ttcagtttct gtcatatctt cgtttggatc
     7921 accccattta tacatggctt tcgcagaaga agcaatatag acatggttac tgtcaagaac
     7981 cgatatcgga ccgagttttc ctgcacctat cacaagccgc tcccaccagg tctgaccgcc
     8041 gtcagttgtg tcaattgtgc agggcttgaa attaaagccg gaaccgcaga aacctgctat
     8101 ccagatctca tccaccgcac caccgtccat atccataaca ctttccatcg agaaagttcc
     8161 tgaaggcaca gttatgtcaa aactgttact tttccctgta aatgtcgcaa aattgtcatc
     8221 gctccagaca atcggtttct gtccggttga agagtcattt tttgcatcat catcataaaa
     8281 ttgaagaaaa atacggccgc ttgatg
//
LOCUS       DAOOYB010000122         8287 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_9558_length_8287_cov_9.820578, whole genome shotgun sequence.
ACCESSION   DAOOYB010000122 DAOOYB010000000
VERSION     DAOOYB010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8287
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9558_length_8287_cov_9.820578"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="PL056_08210"
     CDS             <1..472
                     /locus_tag="PL056_08210"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPM47038.1"
                     /translation="VKYRNAEETEKNTSLPVDLAEVISFHAAEFEKRGIEVKLIHCEN
                     IVIPITLSSFKEVMLNLFKNGWEELENGGKILITVSVKNDRCVIVVEDSGKGIPEIER
                     EQVFRNFYTKKPGGTGIGLSHSRKLAVEAGGKLYAADSSLGGVAMVLEIPLKTK"
     gene            564..>1306
                     /locus_tag="PL056_08215"
     CDS             564..>1306
                     /locus_tag="PL056_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47039.1"
                     /translation="MKKYLFFLLFTVSFSLTGLEIHYSDGTFEKIVKVTGYSAVKNPL
                     SRTIAVPGEVIRLNLGSEIFSVIEGSNGNFPVYLFGDVPVIAGDMLFWRGTVKIDEME
                     RKYGLKLHEILPSYPLFSFKVLKGDSVEVASEIVRNGDGYAFPNLIKEAKLSYVPESE
                     VEDPYFDVQWHLENTGMVNDYYGSDVKILKNADIKFSQTLEFLKKSMHEVDDSVKVAI
                     MDTGIVPDHEDLTNIEPGYDAFNDTEGGYP"
     assembly_gap    1307..1406
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1407..2948
                     /locus_tag="PL056_08220"
     CDS             <1407..2948
                     /locus_tag="PL056_08220"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPM47040.1"
                     /translation="ADYGTVPITAAERESHQNFMQNGRGGLGGVIVYASGNDGVDSSY
                     SKLLTNVFSFERNEKTVEHTVVVVNASSAWDTRVVYSNYGPASSVIAPSLSQNPMVGI
                     ATTTIPGYGDYMTDYTKVFSGTSAAAPLVSGYFGTIFSVNPELTLEDAIEIMKLSSDK
                     IYPETGSWDKNGFSVKFGWGRINLEKAVRLAMDLDMCESVKTESCGNHIDDDCDGYVD
                     EECAVELLAGTPCESVEDCINGSLVEADAECITSKKYWKFNDGYCMRKTNGAPCPDGT
                     KAFDYASDGNNYICAAECSEANPCKRDGYYCSNDVLGVCIPHCKGDKDCNEGSYCDEY
                     GECVKNPSALGGSCANDDECQGSMWCLTYFQDGYCTADCADNDDSYCPDDGKCVTRKA
                     GTGNVDICLSACSSDSHCRDNEYYICHGKISNKEGVCFRKCRNDGDCMDPDATCGEDG
                     RCAVAGWEGWPEEVTDEDAVDTEITDTDVDDETVDIAEVPDEEKEEEKKSEKDNSFGC
                     TIINF"
     gene            2948..5089
                     /locus_tag="PL056_08225"
     CDS             2948..5089
                     /locus_tag="PL056_08225"
                     /inference="COORDINATES: protein motif:HMM:NF012551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HPM47041.1"
                     /translation="MKRISTAFCILFPFLSLFSSTLNISFTSESGGPAFKYVAVYDLA
                     SASELASADLSATDKYSAEVPDNTDVFVLGIASSGKSNSGFTVHEFMPVSKAVNSGTG
                     EKTVKLVTEPAFQLIFQNAEFAEGELFAVDMDDRFLTVFKLDVTNSSNKSLPAITLPL
                     GKCYAIYILKELPVSGKMIYRMDNKGEGFCSDSQGNLMIDTDKAVAESVIARYEDLIS
                     ETPAATESHLEKLNEINDLYDKGDYKTSAGEAVMQSEELIFEEARINIDMYRKGKVSV
                     KINDLSGNPVAGIKVEAVPLRTDYKRGVLAGIYDLDPSIFFNAVADGFSFATVGTLWL
                     DVEPTDDNYRWSYIDEVAGNRKIYDMGYELFGHSIIYLLDFVMPEYLKSMNAGSLNEE
                     ISEHTQALAQHYDGMINEWLVINEAHSISASYGFSRKEITEFTNTVIKVLNEHDPDTI
                     KTINAAPDYFGQSMFTEMFMVDRDALFSMPVYDYFKDLIENGVDFDVIGQQMYNGGCI
                     TLFKDYGFADSASAIPVYDLVEMRRMLRKLQTLDKNVYVTEISVPASMNSSCPGMGYW
                     RKEWSEEVQAQFLERLLAVIMGEKDVRLVNYWDMQDKGSFIYKGGLMDEDGRKKPAYE
                     VASKAFGNFLKTVELVSGVDGIAELKLYAGEYEINIDDGKKIEQITVGESQEAQLEYQ
                     PDLFPEEETDEETVDNETQKGKENGCTLLGI"
     gene            5280..5651
                     /gene="rpsL"
                     /locus_tag="PL056_08230"
     CDS             5280..5651
                     /gene="rpsL"
                     /locus_tag="PL056_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010938593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HPM47042.1"
                     /translation="MPTINQLIRKGRKDQKNKSKCPALVACPQRKGVCVRVYTTTPKK
                     PNSALRKVARVRLTSGFEVACYIPGEGHNLQEHSVVLVRGGRVKDLPGVRYHIVRGTH
                     DTSGVDGRKQSRSKYGAKRPK"
     gene            5669..6139
                     /gene="rpsG"
                     /locus_tag="PL056_08235"
     CDS             5669..6139
                     /gene="rpsG"
                     /locus_tag="PL056_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HPM47043.1"
                     /translation="MPRRKCVSKREVSADPKYGDVLLQKFINKIMLKGKKSTAETIVY
                     NAIETAAKGKKDSPVDVFKIALENIRPQVMVKSTRIGGATYQVPTEISLENSQAIGMK
                     WMITFSRKRSEKGMALKLAGEIDEAFNKRGNSFKRKDDTHKMAEANKAFAHFKW"
     gene            6172..8250
                     /gene="fusA"
                     /locus_tag="PL056_08240"
     CDS             6172..8250
                     /gene="fusA"
                     /locus_tag="PL056_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HPM47044.1"
                     /translation="MKSNIALEDIRNIGIMAHIDAGKTTTTERILFYTGVSRKMGEVH
                     EGTAVMDWMEQEQERGITITSAATTCYWKNKRINIIDTPGHVDFTIEVERSLRVLDGA
                     IAVFCAVGGVEPQSETVWRQADRYKVPRLAFVNKMDRVGADFFNVIRMMELKLGARAL
                     ALQIPVGKEDTFTGIIDLVEMNTRYYDSDVLGSTYRVENEIPSGYEDVVNEHREKLFE
                     ALSDFDEDIMTLFLESSDNIEPIKIKNALRKAVIKNAVFPVFCGSSFKNKGVQLLLDA
                     VLDYLPSPLDLPPIKVEGLKKDKEKFVAPTDDVPGALLFKIMSDPFVGELSFVRVYCG
                     ELKVGQQYYNPLTEKVERIGRLLRMHANKREDVDTIPSGNIGAVVGLKHATTGSTLCS
                     KQDPFLFEKIKFPEPVISIAIEPKTKADEDKLSYSLEKLAKEDPSFRVRTDEETGQTL
                     ISGMGEFHLEVLVERMRREFKVDANVGSPTVSYRETFSKPVRMEHKLVKQTGGKGQFA
                     HVIMEFEPLPAGSGVVFESNIKGGAIPSLFINAVEKGIKTAVYGGRYGYPVVDVKVKL
                     MDGSFHEVDSSEMAFKAAAVSCFKDAEEKSNPKILEPVMKVEISVPEQYMGDVIGNLQ
                     SKRAEIEGYENMAGGIRMINVSVPLSEMFGYMTSLRSQTQGRGTFTMEFSHYKEIPEN
                     IKKQKFGITF"
BASE COUNT         2565 a         1462 c         2032 g         2128 t
ORIGIN      
        1 ggttaaatac agaaatgctg aagaaacaga aaagaataca tctctaccgg tagatcttgc
       61 ggaggtgata tcttttcatg ccgctgaatt tgaaaaaagg gggattgaag taaaactgat
      121 ccattgtgaa aacattgtta tcccgataac gctctcatca tttaaagagg taatgctgaa
      181 tctgttcaaa aatggctggg aggagcttga aaacggggga aagatactga taactgtttc
      241 tgttaaaaat gacaggtgtg ttatagttgt tgaggattcg ggcaaaggga ttcccgaaat
      301 tgaaagagag caggttttca ggaattttta tacaaaaaaa cccggaggaa ccggtatagg
      361 attaagccac tcaagaaaac ttgcagtcga agcgggcggt aaactttatg ccgcagattc
      421 atctctggga ggtgtcgcca tggttttgga aattcctttg aaaacaaagt aatatgctgt
      481 tattattaaa taaatttatc aggcatcgtt gttgctctaa aattagaggt gttgaggtgt
      541 taatgttatt taaggagtcc gatatgaaaa agtatttgtt tttcctgctt tttacagttt
      601 cattttcttt aacaggactt gagatccatt acagtgacgg aacttttgaa aagatcgtta
      661 aagttacggg ctacagtgct gtaaaaaatc cgctctcaag aaccattgct gttccgggtg
      721 aggtcatccg gcttaatctc ggcagtgaaa tattttctgt cattgaaggc agcaatggaa
      781 attttccggt ctatcttttt ggagatgttc ctgtaattgc aggtgatatg cttttctgga
      841 gaggaactgt gaaaattgat gaaatggaga gaaaatacgg acttaaactg catgagatac
      901 ttccctctta ccctcttttc agctttaagg tgttgaaagg tgacagtgtt gaggtcgcgt
      961 cagaaatagt tagaaacggt gacggatacg cttttcccaa ccttataaaa gaagcgaagt
     1021 tgagctatgt tcccgaaagc gaagttgaag acccttattt tgatgtccag tggcatctgg
     1081 aaaacaccgg aatggttaac gattattacg gcagtgatgt aaaaatactg aagaatgcag
     1141 acattaaatt ctcacagaca cttgaattct taaaaaagag catgcatgag gtcgatgact
     1201 ctgttaaagt ggctataatg gataccggaa ttgttcctga tcacgaagat cttacaaata
     1261 ttgagccggg atacgatgcg ttcaatgata cagaaggggg atacccnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnngcag attacggcac ggttcccata actgccgctg
     1441 aaagagagtc acatcagaac ttcatgcaga atggaagagg cggtctgggc ggtgttattg
     1501 tatatgcttc aggaaatgac ggagttgatt caagttattc gaagcttctt accaatgttt
     1561 tttcttttga aaggaatgaa aaaactgttg agcatacagt tgttgtcgta aatgcttcat
     1621 cggcatggga cacaagggtt gtatattcaa attacggtcc ggcttcgtct gtcattgctc
     1681 cgtctctttc acagaatccg atggtcggca ttgctacaac gaccattccg gggtatggtg
     1741 actatatgac cgattataca aaagtctttt caggaacttc tgcggctgcg cctcttgttt
     1801 cggggtattt cggaacaatt ttcagtgtca acccggaact tacccttgaa gatgcgatcg
     1861 aaataatgaa gttaagttcc gacaagatat atcctgaaac cggctcatgg gataagaacg
     1921 gattcagcgt aaagttcggc tggggaagga tcaaccttga aaaagcagtc cggcttgcaa
     1981 tggatcttga tatgtgtgaa tcggtcaaaa ccgaatcctg cgggaaccac attgatgacg
     2041 actgtgacgg atatgttgac gaagaatgtg cagttgagct ccttgcagga acaccttgcg
     2101 aaagcgttga ggattgtata aacggatctc ttgttgaagc tgatgcggag tgcataacaa
     2161 gcaagaaata ctggaaattc aatgatggat actgcatgag aaagactaac ggagctccct
     2221 gtcctgacgg aaccaaagct tttgattatg catctgatgg caataattat atctgtgcgg
     2281 ctgaatgcag cgaagcaaat ccatgtaaaa gggatgggta ctactgcagt aacgatgttc
     2341 tcggagtctg cattcctcac tgcaaaggtg ataaagactg caatgaagga agttactgcg
     2401 atgaatacgg tgaatgtgta aaaaacccgt cggctctcgg cggaagctgt gcaaatgatg
     2461 atgaatgtca gggctccatg tggtgtctca cctattttca ggatgggtac tgtacggcgg
     2521 attgtgctga caacgacgac tcatactgcc ccgatgacgg aaaatgtgtc acaagaaagg
     2581 cgggtaccgg aaatgttgat atatgtcttt cagcatgcag ttccgacagt cactgccggg
     2641 acaatgaata ctacatctgt catggaaaaa tatcgaacaa ggaaggggtc tgtttcagaa
     2701 aatgcaggaa tgacggcgac tgcatggatc cggatgcaac atgcggtgaa gatggaagat
     2761 gtgctgttgc cggatgggag ggatggcctg aggaagtgac agatgaagat gcagttgaca
     2821 ccgagatcac agataccgat gtcgatgacg aaactgttga catagccgaa gttcccgatg
     2881 aagaaaaaga agaagaaaag aaatctgaaa aagacaattc attcggctgc actataataa
     2941 atttctaatg aaaaggattt caactgcttt ttgcatattg tttccgtttt tatcactttt
     3001 ttcatcaact ctaaacattt cgttcacttc agaaagcggg ggtcccgctt ttaaatatgt
     3061 tgctgtgtat gaccttgcaa gtgcgagcga actggcatcg gcagatttgt cggcaactga
     3121 taaatattcg gcagaagtgc ctgataatac tgatgttttt gttctgggga ttgcttcaag
     3181 cggtaaaagc aattcgggat ttacggtgca tgaattcatg cctgtttcaa aggcggtcaa
     3241 ttcgggaact ggtgaaaaaa ccgttaaact tgttactgaa ccggcttttc agctgatatt
     3301 tcagaatgcc gaatttgctg aaggtgaact ttttgcagtc gatatggatg acaggtttct
     3361 gacggttttt aaactggatg ttacaaattc atcaaataaa agtctgccgg cgataactct
     3421 tccgcttgga aaatgttacg cgatctatat tttaaaagag cttcccgttt caggaaaaat
     3481 gatatacaga atggataaca aaggggaggg gttctgctca gattctcagg gaaacctgat
     3541 gatagacact gacaaagcag ttgctgaaag tgtgatagca agatatgaag atcttataag
     3601 tgaaactcct gccgccacag agagccatct tgaaaaactt aatgagatca atgatttgta
     3661 tgacaaaggt gattataaaa cctcggcagg tgaagctgta atgcaatccg aggagcttat
     3721 ctttgaagaa gcacgcatta atattgatat gtatagaaaa gggaaagtgt cggttaaaat
     3781 aaatgatctg tccggaaatc cggtcgccgg tattaaagtt gaggcggttc cgttaagaac
     3841 cgattataaa aggggggttc ttgcagggat ctatgacctt gacccttcaa tttttttcaa
     3901 tgctgttgcg gacggattca gttttgcgac tgtcggaacg ctctggcttg atgttgagcc
     3961 gactgatgac aactaccgtt ggagctatat tgatgaagtg gcaggcaaca gaaagatcta
     4021 tgacatgggc tatgaacttt tcggtcactc gatcatctat cttcttgatt ttgtcatgcc
     4081 ggaatatctg aaaagcatga atgccggatc attgaacgaa gaaatttcgg aacatactca
     4141 agcacttgca cagcattatg acggaatgat aaatgaatgg ctcgtcataa acgaagctca
     4201 ctcgatatct gcttcatatg gattttcaag aaaagagatc acagaattca ccaatacggt
     4261 gataaaagtg ctcaacgaac atgatcccga caccataaaa acaatcaatg ccgctccgga
     4321 ttacttcgga cagagcatgt tcacagaaat gttcatggtc gatagagatg cccttttttc
     4381 aatgccggta tatgattatt tcaaagatct gattgaaaat ggtgttgatt ttgatgtcat
     4441 aggtcagcag atgtataacg gcggctgtat aacacttttt aaagattacg gatttgccga
     4501 ttctgcctcc gcaattcctg tgtacgatct tgttgagatg aggaggatgt taagaaaact
     4561 tcagacactt gacaagaatg tgtatgtgac agaaataagt gtccctgcat cgatgaacag
     4621 cagttgtccc ggtatgggat actggagaaa ggagtggagt gaagaggttc aggcacaatt
     4681 tctggagcgg cttctggcgg tcataatggg cgaaaaggat gtccgccttg taaactactg
     4741 ggatatgcag gataagggct cattcattta taaaggcgga ctcatggatg aggacggaag
     4801 aaaaaagccg gcatacgaag ttgcttcaaa ggcgtttggc aatttcctga aaactgttga
     4861 gctggtttca ggagtggacg gcattgctga gttaaagctc tatgccggag aatatgagat
     4921 aaacatagat gacggaaaaa agatagaaca gataactgtc ggagaatcgc aggaagctca
     4981 acttgaatat cagccggact tatttccgga agaggaaaca gatgaagaga cagttgacaa
     5041 tgaaactcaa aaaggaaaag agaacggctg tactttgctc ggtatatgat cagaagaata
     5101 atcttcaaat ctatggaaaa aacacttaaa aaataaaaac cgaaaaagcg cactgttgaa
     5161 aataatcaaa taaatcaaag tgttaaaata aaaaagaaac aaatttttga cacatcaaag
     5221 gtgctccata taatctcgga agttttttgg taatggttta aaagaggagt atcttaaaca
     5281 tgccgaccat taatcagttg ataagaaaag gtcgtaaaga ccagaagaac aaaagcaagt
     5341 gcccggcgct tgttgcgtgt ccgcagagaa aaggtgtttg tgtacgtgtt tacaccacaa
     5401 ccccgaagaa gccgaactca gcacttagaa aagttgcaag ggttcgtctg acaagcggtt
     5461 ttgaagttgc ctgctacatt cccggtgaag gccacaatct tcaggaacac agtgttgttc
     5521 ttgtcagagg cggcagggta aaagacctcc ccggtgtgcg ttatcacatc gtaagaggaa
     5581 cccacgacac atcaggtgtt gacggcagaa aacagagtcg ttcgaaatac ggcgctaaga
     5641 gaccaaagta gtaaaggggg attaagcaat gcctaggaga aaatgtgttt ccaaaagaga
     5701 agtgagtgca gatcctaagt acggagatgt ccttcttcag aaattcatca acaagatcat
     5761 gcttaaggga aaaaagagca cggccgaaac gatcgtttac aatgcgatag aaaccgctgc
     5821 aaaagggaaa aaggacagcc ctgttgatgt ttttaagata gctcttgaga acatcagacc
     5881 gcaggtaatg gtcaaatcga ccaggatcgg cggtgcaact tatcaggttc cgacagagat
     5941 ttcccttgag aacagtcagg cgatcggtat gaagtggatg atcacttttt cgcgcaaaag
     6001 aagcgaaaag ggaatggcac tcaaacttgc cggcgagatc gatgaagctt tcaataaaag
     6061 aggaaattct tttaagagga aagacgatac ccacaaaatg gctgaagcaa ataaagcttt
     6121 cgcgcacttc aagtggtagg attttaaaag gagatttttg agttgaacac cgtgaaaagt
     6181 aacatagccc tggaggacat ccgcaatata ggcataatgg cccatattga tgcgggtaag
     6241 actaccacca cggaaagaat acttttctat accggtgtgt cacggaaaat gggagaagtt
     6301 catgaaggca ctgccgtaat ggactggatg gaacaggagc aggaacgcgg cataacaata
     6361 acttcggccg ccacgacatg ttactggaag aacaaaagga taaacataat agacactccg
     6421 ggacatgttg atttcacgat cgaagttgaa agatctctca gagtccttga cggagcgatc
     6481 gccgtattct gtgctgtagg gggagttgag ccacagtctg aaaccgtatg gagacaggcc
     6541 gacaggtata aagttccgag actcgcattt gtcaacaaaa tggatcgtgt cggagcggac
     6601 ttcttcaatg ttatcagaat gatggagctc aagctcggag caagagcgct tgcacttcag
     6661 attccggtgg gcaaagagga tacattcacg ggaataatag acctcgtaga aatgaacacc
     6721 cgttattatg acagcgatgt tctgggttcg acctacaggg ttgagaatga aataccttca
     6781 ggttatgaag atgtggtgaa cgaacacaga gagaaacttt ttgaagcgct ttctgatttt
     6841 gatgaagata taatgaccct ttttcttgaa agttcagaca atattgaacc gataaaaata
     6901 aagaatgcat tgagaaaggc ggtaataaaa aacgctgttt tcccggtatt ctgcggcagt
     6961 tcttttaaaa ataaaggtgt tcagcttctt cttgacgcgg tgcttgatta tcttccgtct
     7021 cctctggatc tgcctcccat taaagttgaa ggtcttaaaa aagataaaga aaaatttgtt
     7081 gctccgacgg atgatgttcc cggtgcgctt cttttcaaaa taatgtcaga tccttttgtc
     7141 ggtgaactca gttttgtaag ggtttactgc ggcgagctta aggttggtca gcagtactat
     7201 aatcctctta cagaaaaagt tgagcggata gggcggcttc tgagaatgca tgcaaacaaa
     7261 agagaagatg ttgacacgat cccttccggt aatatcggtg ccgttgtcgg tctaaaacat
     7321 gcaacaaccg gatctacact ctgttcaaag caggatccgt tcctgttcga aaagataaaa
     7381 tttcctgagc cggtcataag cattgcaata gagcccaaga caaaagccga tgaagacaag
     7441 ctctcgtatt cattggagaa acttgctaaa gaggatccga gcttcagagt gcgcaccgat
     7501 gaagaaactg gacagactct catatccgga atgggtgagt ttcatcttga agttcttgtt
     7561 gagagaatgc gcagggaatt taaagtcgat gcaaatgtgg gaagtccgac cgtaagttat
     7621 cgtgaaactt tttcaaagcc ggtcagaatg gagcacaaac tcgttaagca gactggcggc
     7681 aaaggacagt ttgcacatgt tattatggag tttgaaccac ttcctgccgg aagcggagtt
     7741 gtttttgaga gtaacataaa gggaggagct attccttctt tgtttataaa tgcggttgaa
     7801 aaagggataa agactgctgt ttacggcgga agatacggat atcctgtggt tgatgtaaaa
     7861 gtaaagctta tggatggctc ttttcatgaa gtcgattcat ctgaaatggc tttcaaggca
     7921 gcagcggtaa gctgttttaa agatgcggaa gaaaaatcaa atccaaaaat acttgagccg
     7981 gtcatgaagg ttgagataag cgttcccgaa cagtacatgg gggatgtcat cggaaatctt
     8041 cagtcaaaga gagcggagat agaaggatac gaaaacatgg caggcgggat cagaatgata
     8101 aatgtttcgg taccgctttc agaaatgttc ggatatatga caagcttaag gtctcagacc
     8161 cagggcagag gaacttttac aatggagttt tcccattaca aggagattcc tgaaaatatt
     8221 aagaagcaga agtttggcat aactttttaa aaggagagta tgatggcaaa agcgaagttt
     8281 gaaaggg
//
LOCUS       DAOOYB010000123         8261 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_9602_length_8261_cov_12.555935, whole genome shotgun sequence.
ACCESSION   DAOOYB010000123 DAOOYB010000000
VERSION     DAOOYB010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8261
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9602_length_8261_cov_12.555935"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            147..2903
                     /gene="ileS"
                     /locus_tag="PL056_08245"
     CDS             147..2903
                     /gene="ileS"
                     /locus_tag="PL056_08245"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HPM47045.1"
                     /translation="MDYGKTLNLPQTDFPMKANLPVKEQEILKNWENIQIYFKMIKKN
                     SDKNSFIMHDGPPYANGSLHDGHVLNKILKDIVVKYKNMSGHLATFRPGWDCHGLPIE
                     LKVAEKLGSKKKEMSKSDFLKECKNFAINAIDIQRKEFKRLGVFADWEKPYTTLSPEY
                     EGCIAHQFANVVESGALFKSKKPVHWCPSCETALAEAEIEYEDHTSPSIYVKFPLIDK
                     LTELENKSVNFVIWTTTPWTLPANLAIALHPEIEYSAVKFNEEVFIIASALKEKFLAA
                     TGHSDAETVMTFNAMRFEGMKTKHPFIDRESVIVYADYVTTDTGTGCVHTAPGHGADD
                     YLTGLKYGLPAYAPVDDRGVLTEDVPYFSGLHVFKANPVIVEHLFKEGFLLNKPGEMI
                     SHSYPHCWRCHKPVIFRATEQWFISMDETNLRKRCLEEIEKVELIPEWGRKRITPMLE
                     NSPNWCVSRQRLWGVPIIAFSCSDCGEKIVDGKIAHKVADEISVKGVSVWHEKEPSEF
                     LPEGFTCPKCSGRNIKKESDILDVWFDSGVSWAAVLDGSKGYEYPCDLYLEGSDQHRG
                     WFQSSLKLSVLIKDKAPYKKVLTHGFVVDGKGEKLSKSKGNFVPPDKRINTLGAEILR
                     LWVAAEDYRDDIRISDNIIEGFATAYRKIRNTMRFMFGFINDFDPDTMKYDINELTSL
                     DKWAYLTWKSKLELVLSKYENFEFHRVYHEILDFFTVAMSSVYLDIIKDRYTMKADDP
                     RRRKSQFTVYQILKDVITALAPILSFTSEEAYGFLKGDKKESVFLEDMPAVSLNDEEK
                     GFLKKWDVLLKVREAVQKQLETLRMQKTIGHSLDAAVDLYFENIPLLDEELNSLESIF
                     IVSGVDKAASSEGLQKVEDISVWMSVRVAPGEKCPRCWKKRELKNFSDELQGICESCY
                     EAVR"
     gene            2900..3406
                     /gene="lspA"
                     /locus_tag="PL056_08250"
     CDS             2900..3406
                     /gene="lspA"
                     /locus_tag="PL056_08250"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HPM47046.1"
                     /translation="MNFKKFEWMNALYLLIITLPVVVLDQATKFWAITLRNGGQVVVF
                     ESWWSFIYAENRGALWGIGGNFPDFYRKLIFLGISTVITLFIVYLLFTYAENRLIKVV
                     YGFVLGGALGNLFDRYFRGYVIDFIDWHAGDYYHWPTFNIADAAIVVAVALLLIELLF
                     FQKKKDSK"
     gene            3403..4233
                     /gene="lgt"
                     /locus_tag="PL056_08255"
     CDS             3403..4233
                     /gene="lgt"
                     /locus_tag="PL056_08255"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HPM47047.1"
                     /translation="MKPILFSIGPLDVSSYYFMGIIGLLGGFFFNYWETKRRNLDVKL
                     SIYGAVWVSIFGYIGARAMHIVFDGFLEIYIQKPLAIFAFWKGGLAFYGTVIFGLPAA
                     LWWFRYHKAPFLKYLDNYTYGIAFGVGIGRIGCYLNGCCFGEISSSSIASQFIKYGLS
                     AKNQFARDILLNLKEMPYPVFPSQLISFFANMVIFLFLWIFVRKRYKKDGSVFGIWMI
                     LYGIFRFIIEIFRADDRGMFFNDLLSSSQIIAIISIIAGILIIKLPDKKEKSVSQSHS
                     "
     gene            complement(4218..5582)
                     /locus_tag="PL056_08260"
     CDS             complement(4218..5582)
                     /locus_tag="PL056_08260"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HPM47048.1"
                     /translation="MKIFVIIIFLVFTASCEIEKPEKSEYEPFVCSEFPESRVFFPEE
                     KISFVFNMDVNPNSLSGFTVKEISSENEIPVEISGDTVSILPPLPAESSLSVTISSAL
                     KSIDNRPLMTGDGFSENKELFEITFETGVKLPEIETIIPSENQSSTVAIKFDGNIEIK
                     FKDVDPVPSDMMKIEDWIVIVYDEPVGKINISKATAVERDTELEDISVTLPSKKPEKG
                     DLSVEYSASDTEIIVTISDESAIAVSVNGVNFMCEKKCSAVLKDLLPETYYSVNTDVY
                     TTTGKKHDTAEIITGEEKPHVIISEVMHTPSLEPEKNWEFVEIFNSGNIDFDLENCFI
                     DDKNDGKGIDPLILKDPERNLILQPGELAVITGNEAAFSDVAGSALWLIVDDTTIADA
                     GLTSTETIQIICNRNDLMVLEAHADPSGLKTERGYSFNSDKYGNICSSSVEGGTPGKY
                     YECD"
     gene            complement(5579..6277)
                     /locus_tag="PL056_08265"
     CDS             complement(5579..6277)
                     /locus_tag="PL056_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47049.1"
                     /translation="MKQLSTLLKKMRFTALVILTIHSGGVLSAQETALESVASHLKGF
                     KITAEMLEKKYSCMTLEQLLKKGLAGINDLEKAVVSARKNTRAAGLLPEISAWSKYKN
                     DEKLYLYQRNNIAVGKDYITIGPDDNNTTYGDLTSFEVGGRIKFDLTKLLYNSDTIKF
                     SDQQQKLYFLRIEMIEKLSSVYFFNAMLNALKNLKIEVPQEKMILYEIIDRKHNGWLK
                     SFAGIDLYECGEKK"
     gene            complement(6210..6494)
                     /locus_tag="PL056_08270"
     CDS             complement(6210..6494)
                     /locus_tag="PL056_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47050.1"
                     /translation="MFSTEMRITKELGNYHYGWFCLFSFDFGHPDGLNIRTPRLQEEQ
                     FENIFISSEEMDEIRDQINYMLEEEEEENETVIDPAEENEIHGSGDPDDP"
     gene            6670..7776
                     /locus_tag="PL056_08275"
     CDS             6670..7776
                     /locus_tag="PL056_08275"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPM47051.1"
                     /translation="MIRFLDLSRQYLQLKKEIDEAVLRVIAEGSFVKGSDVREFEKEF
                     ADYLKADHCIACANGTDALTALIKCLELPFGSTVVVPANTFVATAESVISNGLKVKFA
                     DIDEDYTISPESVESVMTEDVSAVIAVHLYGQPAKIPELKKITAKWNVPLIEDAAQSH
                     GAMIGNVKAGSLGDGAAFSFYPGKVLGAMGDAGAVVVNDDILAKKVRQYCDHGRTEKY
                     RHEVAGINSRMDTIQAAVLNVKLKYLENWIGMRNNIAEAYLNLLKDVEGLKLPLKREG
                     LRHAWHLFVVRHVRRDELKEYLRENGVESGIHYPISLPEQPVFKDHMIYAGEYKAVKY
                     SSELLSLPMGEHLSVENIEKIAELLKKFHKKNNF"
     gene            7940..>8261
                     /locus_tag="PL056_08280"
     CDS             7940..>8261
                     /locus_tag="PL056_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47052.1"
                     /translation="MKKLMIAMIALSVLFGFVACGDDPIPSQPDSEADTGQDDDGNTG
                     NTGDTGNTGNTGNSGNTGDTGNTGDTGNTGNTGNTGDCDTDNLGKSCTTDTDCGKCMI
                     CITNG"
BASE COUNT         2479 a         1528 c         1783 g         2471 t
ORIGIN      
        1 tttgttccgc tataaattta tacataaaaa tcaactttgt caatattaat gaacaaaaca
       61 aatgcaaatc aaaccttgac tttatggtat ccagagaata aaattccccg catttttgta
      121 actgcttact taatctggag ttatcaatgg attatggaaa aacactgaat cttccccaaa
      181 ccgatttccc aatgaaggcc aacctccccg ttaaagaaca ggagattctg aaaaactggg
      241 aaaacatcca aatttatttt aagatgatta aaaagaattc ggacaaaaat tctttcataa
      301 tgcatgacgg acctccatat gccaatggaa gtcttcatga cggccatgtt ctgaacaaga
      361 tactgaaaga tattgttgtc aaatataaga acatgtcggg acatcttgcc acattcagac
      421 ccggatggga ttgtcacggt cttccgatag agctgaaggt tgccgaaaag cttggatcga
      481 agaagaagga aatgtctaaa tcagactttc tcaaggaatg taagaatttt gcaataaatg
      541 caattgacat tcaaagaaaa gagtttaaac ggctgggagt ttttgcagac tgggaaaaac
      601 cctacactac gctttctcct gaatacgaag gatgcatagc ccatcaattt gcaaatgttg
      661 ttgagtccgg tgctcttttt aaatcaaaga aacccgttca ctggtgtccg agctgtgaaa
      721 ctgcacttgc tgaagccgag atagaatatg aagaccacac atctccttca atatatgtga
      781 aatttccgtt gatagacaaa ttgacggagc tcgaaaataa atcggtgaat tttgtgatct
      841 ggaccacaac tccgtggact cttccggcaa atcttgccat tgctcttcat cctgaaattg
      901 aatacagtgc cgttaaattt aacgaagagg tttttatcat tgcttctgct ttgaaagaaa
      961 aatttcttgc tgcaacggga catagtgatg ctgaaactgt aatgactttc aatgcaatga
     1021 gatttgaggg tatgaaaacc aaacatcctt tcatagacag agaatcggtc atagtttatg
     1081 cagattatgt tacgacagat acaggaaccg gttgcgttca tactgctccg ggtcatggag
     1141 ccgacgacta tctgacaggt cttaaatacg ggcttcccgc ttatgctccg gtcgatgaca
     1201 gaggtgttct gacagaagat gttccatatt ttagcggtct tcatgttttc aaagcaaatc
     1261 ccgtgatagt ggaacatctt ttcaaggaag gttttcttct gaataagccg ggcgaaatga
     1321 taagtcacag ctatccacac tgctggagat gtcacaagcc ggtgattttc agggcgacag
     1381 agcagtggtt catttccatg gacgaaacga atttgaggaa aagatgcctt gaagagatag
     1441 aaaaagttga acttattccg gaatggggaa gaaaaaggat aacaccgatg cttgagaatt
     1501 cgccgaactg gtgtgtttcg agacagcggt tatggggtgt gccgattatt gctttttcat
     1561 gcagtgactg cggggaaaag atagttgatg gaaagattgc ccataaagtt gcggatgaaa
     1621 tatctgtcaa aggagtatct gtctggcatg aaaaagagcc gtcagaattt cttcctgaag
     1681 gtttcacatg tccgaaatgt tccggcagga atataaaaaa ggaaagcgac atcctggatg
     1741 tctggtttga cagtggagtg agctgggcgg cagtacttga cgggagcaaa ggttatgaat
     1801 atccgtgcga tctttatctt gagggaagcg accagcacag aggatggttc cagtcttcat
     1861 tgaaactgag cgttcttatt aaagacaagg ccccctacaa aaaagtactc actcacggtt
     1921 ttgttgttga cggaaaaggg gagaagcttt caaaaagcaa gggcaatttt gttccgcccg
     1981 acaaaaggat caacactctt ggtgcggaaa ttctcagact atgggttgcc gccgaagatt
     2041 atcgggacga cattagaata agtgacaaca taattgaagg atttgcaact gcctacagga
     2101 agatcagaaa cacaatgaga ttcatgttcg gatttatcaa tgattttgat cccgacacaa
     2161 tgaaatatga tattaatgaa ctgacttctc ttgataaatg ggcttatctg acatggaaaa
     2221 gcaagcttga acttgttctc tcaaaatatg aaaatttcga gtttcacagg gtttatcacg
     2281 aaattctcga ttttttcaca gttgcaatga gctcagtcta tctcgatatc ataaaagacc
     2341 gttatacaat gaaagctgac gatccaagaa ggagaaagtc gcagttcacg gtctatcaaa
     2401 tacttaaaga tgtcataaca gcccttgctc ctattctgag cttcacttct gaagaggcat
     2461 acggttttct taaaggtgac aaaaaagaga gtgtttttct tgaagatatg ccggctgttt
     2521 ctttgaacga tgaggaaaaa gggtttttaa aaaaatggga tgtgcttctc aaggttcgtg
     2581 aagcagttca gaaacagctc gaaacactta gaatgcagaa gacgataggt cattcacttg
     2641 atgcggcggt tgatctctat tttgaaaaca ttcctttgct ggatgaggag ttgaattctc
     2701 tggaatccat attcatagtt tccggtgttg acaaagcggc gtcaagcgaa ggattgcaga
     2761 aagttgaaga tataagtgtc tggatgtcag tcagagtcgc tccgggtgag aaatgtccga
     2821 gatgctggaa gaaaagggag ttgaagaatt tctcagatga acttcagggg atatgtgaat
     2881 cctgttatga ggcggtcaga tgaattttaa aaaattcgaa tggatgaatg cactctatct
     2941 tctgataata acgcttccgg tagtggtact tgatcaagcc acaaagttct gggccataac
     3001 gctcaggaac ggtggacagg ttgttgtatt tgaaagctgg tggagtttta tttatgctga
     3061 aaaccgcggt gcattatggg ggatcggagg caactttccc gatttttaca ggaaactgat
     3121 ctttcttgga atctcaactg taataaccct ttttattgtc tatctgctct ttacttatgc
     3181 agaaaacaga ctgataaaag tagtttacgg ttttgttctc gggggagcgc tcgggaatct
     3241 ctttgaccgt tatttcaggg gctatgtcat tgattttata gactggcatg caggagatta
     3301 ctatcactgg ccgacattta atattgctga cgcggcaata gttgttgcag ttgcacttct
     3361 tttaatagag cttctttttt tccagaaaaa gaaggactca aaatgaaacc gattcttttt
     3421 tctatcggac cgcttgatgt ttcaagctac tatttcatgg gaattatcgg tctgctcggc
     3481 ggatttttct ttaattactg ggaaacgaaa agacggaacc ttgatgtgaa actttcaatc
     3541 tacggagctg tctgggtcag cattttcgga tatatcggtg caagggcaat gcacattgtt
     3601 tttgacggat ttcttgaaat atatattcag aagccgcttg caatattcgc attctggaaa
     3661 gggggtttgg ctttttacgg aactgtgata ttcggtcttc ccgccgccct gtggtggttc
     3721 agatatcaca aagccccatt tctgaaatat cttgacaact atacatacgg aatagctttc
     3781 ggtgtcggca tagggaggat cggctgttat ctgaacggat gctgtttcgg cgaaatttca
     3841 tcttcatcaa tcgccagtca gttcataaaa tacggactca gcgcaaaaaa tcagtttgcg
     3901 cgggacattc ttctgaattt aaaggaaatg ccctatccgg tttttccttc tcaactcata
     3961 tctttttttg caaatatggt catatttctt tttctttgga tatttgtaag aaaaagatat
     4021 aaaaaagatg gttctgtatt tgggatctgg atgatacttt acggtatttt cagattcata
     4081 atagagattt tcagagccga cgacagagga atgttcttca atgatcttct ttcctcatca
     4141 cagataattg ctataatttc aataatagcg ggaattttga taatcaagct cccggataag
     4201 aaagaaaagt cagtttctca atcgcattca taatattttc ccggagttcc tccttccaca
     4261 gatgaagaac agatgtttcc gtatttgtca gaattgaatg aatatcctct ttcggttttc
     4321 agtccggacg gatctgcatg tgcttcaagc accatcaggt catttctgtt gcagattatc
     4381 tggattgttt cagttgaagt gagtcccgca tcggcaatgg tagtgtcatc gacaataagc
     4441 cataaagcac tgccggcaac atcagagaaa gccgcttcat ttcctgttat tacggcaagt
     4501 tcccccggtt gtaatatcaa atttctttca ggatctttaa gaataagcgg atcaattcct
     4561 tttccgtcat ttttatcatc aatgaagcag ttttcaaggt caaaatcaat gttgccggaa
     4621 ttaaatattt caacaaattc ccagtttttt tccggctcaa gtgacggggt gtgcattact
     4681 tcagagatga ttacatgcgg tttttcttca ccggtaataa tttcggcagt atcatgcttt
     4741 tttcctgttg ttgtgtaaac atcggtattt actgaataat aagtttcagg caacaggtct
     4801 ttaagaacag ccgaacattt tttttcacac atgaaattca caccgttcac tgaaacagct
     4861 attgccgatt cgtcagaaat agttacaatt atttcagtgt ctgaagccga atattcaacc
     4921 gaaagatcgc ccttttcagg ttttttagaa ggaagcgtta cagaaatatc ttcaagttca
     4981 gtgtctcttt caacagctgt ggccttagaa atgtttattt tcccgacagg ttcatcataa
     5041 acgataacta tccagtcctc tatcttcatc atgtctgaag gcactggatc aacatcctta
     5101 aacttgatct caatatttcc gtcaaatttt atagcaacgg tcgagctttg gttttcagaa
     5161 gggatgatcg tttcaatttc aggaagtttt acgccggttt caaaagtgat ctcgaagagc
     5221 tccttgtttt cagaaaaacc gtctcctgtc atcaaaggtc gattgtcgat cgatttaagt
     5281 gccgagctta ttgtaactga gagtgaagat tccgctggaa ggggaggaag tatcgagaca
     5341 gtatcacctg aaatttccac aggaatttca ttttctgaag agatctcttt tacggtaaac
     5401 ccggaaagtg aattcggatt tacatccatg ttgaacacaa aagatatttt ttcttccggg
     5461 aaaaaaaccc tgctttctgg aaattctgag cagacgaacg gttcatattc agatttttcg
     5521 ggtttctcaa tttcacaaga tgctgtgaag acaagaaata taataattac aaatattttc
     5581 atttcttctc cccgcattcg taaagatcaa tgccggcaaa tgatttaagc catccattgt
     5641 gtttcctgtc tattatttca tataggatca ttttttcctg aggcacttct attttaaggt
     5701 tttttaaggc attaagcatg gcattgaaaa aatatactga actgagcttt tctatcattt
     5761 ctattcttaa gaaatagagt ttctgctgct gatcgctgaa ctttatggtg tcagaattat
     5821 aaaggagctt tgtcagatcg aattttattc ttcctccaac ctcgaatgaa gtgagatcgc
     5881 cataagttgt gttgttgtca tcaggtccga tagtaatgta atcttttcct acagcaatat
     5941 tatttctctg atataaataa agtttttcgt catttttata tttgctccag gcacttattt
     6001 ccgggagtaa tccggctgca cgggtattct ttcttgcaga aaccacggct ttttccagat
     6061 cgtttattcc cgccaatcct tttttaagca gttgctcaag tgtcatgcag ctatatttct
     6121 tttcgagcat ttcagccgtt atcttgaatc ctttaagatg tgaagcaact gattccaaag
     6181 cagtttcctg agcagaaaga acgcctccgc tatggatcgt caggatcacc agagccgtga
     6241 atctcatttt cttcagcagg gtcgataact gtttcatttt cctcctcttc ttcttcaagc
     6301 atataattta tctgatctct tatttcatcc atttcttctg aggatatgaa aatattttca
     6361 aactgctcct cctgcagtcg gggcgttctt atatttaagc catccggatg accgaaatcg
     6421 aatgaaaaaa gacagaacca gccgtaatga tagttcccca gctcttttgt tatccgcatt
     6481 tcggttgaaa acattatctg tagacagaag caaactgaaa ttaattgtac gctaaccatt
     6541 aagcactgaa aaaaacaata aataaacaat gcaataatac aaagtagata tagttgtgtc
     6601 aagaaaaagc atgattttga ctccataaga gttttgcagt atatgtgaag ctgaacgcag
     6661 gaggtgagca tgatcagatt tcttgatcta agtagacaat atctgcaact gaaaaaagag
     6721 atagacgaag cagttctgag agtcattgca gagggatcat ttgttaaagg cagtgatgtc
     6781 agagagttcg aaaaagaatt tgcggactat ctgaaagcag accattgcat agcttgcgca
     6841 aacgggacag atgcgctgac ggcattgata aaatgtctgg aacttccttt cggcagcaca
     6901 gttgttgtgc ctgcaaatac ttttgtggcg actgccgaat cggttatatc taacggattg
     6961 aaagtgaagt tcgctgacat tgatgaagat tatactattt ctcctgaaag tgttgaaagc
     7021 gtaatgaccg aagatgtctc cgctgtcatc gcagtccacc tttacggcca gccggcaaaa
     7081 atcccggagt tgaaaaagat cacagcaaaa tggaatgttc ctctcattga agatgccgca
     7141 cagtcccacg gtgcaatgat cgggaatgtt aaagccggct ctcttggaga tggtgccgct
     7201 ttcagttttt atcctggaaa agtgctgggc gcaatgggag atgcaggcgc agtagttgtt
     7261 aacgatgata ttctggctaa gaaagtaagg cagtactgtg atcacggcag aaccgaaaaa
     7321 tacaggcatg aagttgccgg aataaactca agaatggaca cgatccaggc cgctgttctt
     7381 aatgttaaac ttaagtatct tgagaattgg ataggcatgc gtaacaacat tgcagaagca
     7441 tatttaaatc ttttaaaaga tgtggaggga ttgaaacttc cgctgaaaag agaaggtctc
     7501 cgccatgcct ggcacctttt tgttgtaaga catgttcgga gagatgagtt aaaagaatat
     7561 ttgagagaga acggggtgga atcaggaatt cattatccga tctcccttcc tgagcagccg
     7621 gtatttaagg accatatgat ttatgcaggg gaatataaag cggtaaaata ttcctctgaa
     7681 ctcctttctc tgcccatggg agagcattta agcgttgaaa atattgaaaa aattgcagag
     7741 ttattgaaaa agtttcacaa aaaaaacaac ttttaacgag ttatttgcgt caaattatag
     7801 attgtcagtt cttttataat gcagaatatt gacaaaaatt ttgtgtagat ttttgttgaa
     7861 aaatattaat aagagactat agtggattgt cgatttgttg gtgtagacac tgatttttaa
     7921 tattttggag gtttttaaaa tgaagaaact aatgattgca atgattgcat tgtcagttct
     7981 tttcggtttt gtagcatgtg gtgacgatcc tattccttca cagcccgaca gtgaagctga
     8041 cacaggtcaa gatgatgacg gtaatacggg aaatacaggt gatacaggaa ataccggaaa
     8101 cactggaaat agtggaaaca ccggagatac cggaaacacc ggagataccg gaaatactgg
     8161 aaatacggga aacacaggcg actgtgacac agataatctg ggtaaaagct gtacaactga
     8221 tacagactgc ggaaaatgta tgatctgcat aacaaatgga a
//
LOCUS       DAOOYB010000124         8261 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_9603_length_8261_cov_8.560444, whole genome shotgun sequence.
ACCESSION   DAOOYB010000124 DAOOYB010000000
VERSION     DAOOYB010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8261
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9603_length_8261_cov_8.560444"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /locus_tag="PL056_08285"
     CDS             complement(<1..849)
                     /locus_tag="PL056_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47053.1"
                     /translation="MKISKKLFLFFFFALFLTSCFDNSYGGAPIDPDIPQKLVILHST
                     DTHAKYLPFWMEPNMFDRNMGLTSSNPPCWDLDYSGGASYCNGAYNKETGLYQVYESG
                     KYNYYTKDQLKELGYYDPKNPSLVIEDINEDGICDLLDCQRCWDRKRNNICDDLEDVD
                     GIPGCSVADCEAQPQLRSVTLCWDLNENMDCDKEEDINFDKMCGFDDCAPVFDRNHDR
                     KCDYPYDTINLTWFDENGKSILRKNYDSDSDYNIALTNAEKNSEDRNRDGLCNYSDYR
                     PGLVNTG"
     gene            complement(863..2476)
                     /locus_tag="PL056_08290"
     CDS             complement(863..2476)
                     /locus_tag="PL056_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47054.1"
                     /translation="MKKIKSLLLLSVIFMFLIAAGCNTTSTNNNGDTGNTGDTGNTGN
                     TGNTGDTGDTGNTGDTGNTGDTGNTGNTGNSGDTGNTGNTGDTEPEGEISKIQKGEVA
                     LETEVTFECVVVGIVYNQDDQYNNTNIKGMYVSELISAARPYSGIYVFVKDTAEIDAF
                     AIGDKISVTGAYKEYYESSQIETNSDSVSKLGTADVPTPAVIADPSTVATPFELVEAE
                     WTPTTDHGADGEKYESVFIKVEDVEITNEDLGHGGWEVTDGLAIDKMMYYYDGLRSEG
                     KKFSEIKGVLIYSYDAFRLAPRLADDLVEDSGDTGDTGDTGNTGDTGNTGDTATTIPE
                     IQQGNVTDKAAITVDKVMVISPAHKHSGSPTTWTFFVGTGTAGDYSGAMVYKMVEGST
                     FPYAEGDWLKITGAAGVYETSFDHYQIQNAGQEVGPNEVTKLADSAGTPPAAVTVSAV
                     DLMDKAKHAKYRGSLVKIEDVKIKTAPDSYGNFILENDLQVTAYFTNKNDYSTYTVGT
                     EFTSITGIFDVHFDVPTVHPKVIGNLVKK"
     gene            complement(2594..3628)
                     /locus_tag="PL056_08295"
     CDS             complement(2594..3628)
                     /locus_tag="PL056_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47055.1"
                     /translation="MGSGFFSGLSKILKKNVKDGINSESLKNLGKLANTDVSDLVGDV
                     KAASLLSQIEKDPEKIENYIQLAELYKYYKKLEKAVDVYLGVAHRYLEQKNPSQTIYF
                     INLGLKIMPEHGPLNMFSADMDIRMGRFSDAPDKYRKAADYFIRKNDPMTALYLFRRI
                     KDMNKATTKDLLNMSSLLIRENMCGDALNILLPLNEKLKDNSEQTLKDKEACLMMLHS
                     LKKEDTGILVDLVETRIEMKQFERAVILIKKLVSNDPENINLLKRQAFVYKQMGDMEN
                     YIRTFRIIANIYSKEGNIVYRNIYFHKILKHLPNDVEALTVLKMEDQLRENIDSKIEN
                     TDSKIKMVDN"
     gene            complement(3802..5073)
                     /locus_tag="PL056_08300"
     CDS             complement(3802..5073)
                     /locus_tag="PL056_08300"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HPM47056.1"
                     /translation="MSVNIIFSIFLSFFIFFTGCSNESAQEKDDAEIQDFDSADEFYE
                     DADAAEEMTVKSIKFTISEEYTDIISSPSKHFAFPDVIKLKNGKFMVVYRSGSGHAEV
                     SGKIYAHYSDDGETWSDIPEVVMDDPSIDDRDPSIVVLENGKVAMNWFKYRYPADYTE
                     PWIHRVFFAASDDHGKNFSDPVQVEPGIFDYSGASSLNDEGIWVDNDGDEIVTYASSS
                     HIVEDAGRLIIPGYGGNALNRNSMSKTPKGPIVFFISEDDGKTWHLEPVNAEAPENTW
                     LQEPALLKISELHWILQVRTAYGSSPGGKGDLMQSTSLDGGKTWSGYRSLGFIAHAPE
                     LLKLNNGAIISSFRWLDWELDIEREAVSFVYSLDNGETWSDIIEIEDCGLAECGYPGT
                     VELENNRIFVVYYTPGGAGIRARTISFSESY"
     gene            complement(5064..6062)
                     /locus_tag="PL056_08305"
     CDS             complement(5064..6062)
                     /locus_tag="PL056_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47057.1"
                     /translation="MKKILFIIVMLTLASCINEEYDDLGLTDDSDINLQNDTEDLPDS
                     NGDSGNTGDSGNSGNTGNSGNTGDTGNTGNTGNTGDSGNTGNTGSGHECGNGTVETGE
                     ICDKNSVQCSELDSNFESGMAECKENCTSWDTTNCIEKKPEITPLAVMPAASFEVEYL
                     YNGVEEFNYGANQGNELWNQPIFKTSIDLGTDTYYIPHSLANVHWLAGFYDSQAVQFV
                     QQSYYVPDEYTSQFSYPYVVMGSLKTATVNGAVLNIGIKDSNEVNLIVVEYMNEAECV
                     MLAGYGTLTVNSVNLVPGDAGDFTFTTSKIDLYLPKETPEGNVIADVQNAGWPVCQ"
     gene            complement(6124..7347)
                     /locus_tag="PL056_08310"
     CDS             complement(6124..7347)
                     /locus_tag="PL056_08310"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPM47058.1"
                     /translation="MNNLTTRSKTLAIQMFHSSLEGTVYGMLIMAEVIMRRELKASIL
                     LITMFTMLGSVTNLLSIYFSHILTKYPGKIKTIITVAAMFTRLPMALFFFFNTAEALF
                     VLSALYYLGDTFIKPVQNIYMKMNYARTELGKIYAYSISLNKVFFIAAVYSFGLWMDN
                     NSSIYIAVFSICGLVSFLSFFMLGYVPVSEWMKEKVSERKVDLLIFPTLIAVFKKNRR
                     FLVYEAAFFIYGGGFMVVLAAVPILLVDHLNLSYSIISFGRGVVSAVVIILTIPVFGK
                     LFDKKDPVHVGVLAFSILIAHPALMITAYFLPVEFAKWSVYLSYVSFGAGMAAVTLCW
                     NIGPMFFAKNNMEIPEMTSTHVTLTGIRGLIWPVLGYFLMKLHIIIPFIFSIGFFITA
                     AFIMRTILEKSDQEI"
     gene            complement(7362..7985)
                     /locus_tag="PL056_08315"
     CDS             complement(7362..7985)
                     /locus_tag="PL056_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47059.1"
                     /translation="MKIVILFLLFLNLVPVFAQNSPEWVLVDDSKNIKLYNREMGDAG
                     IKEVKAVATITGTVKEAVEVIFDRKNHPEIFKYIKFADQMKKTAECDWSYNVIDAPMA
                     SDRDYLVKSCEVKNSDGSISVTWEPFEDPKYPVNEKKVRVVINQGYWKFTQVKPDEIR
                     VDYYIYTDPAGSLPLWIKNLANKKAVPDAIFTVEREILKRRDSKARP"
     gene            8111..8187
                     /locus_tag="PL056_08320"
     tRNA            8111..8187
                     /locus_tag="PL056_08320"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8145..8147,aa:Met,seq:cat)
BASE COUNT         2261 a         1777 c         1615 g         2608 t
ORIGIN      
        1 gccggtattt acaagaccgg gacggtaatc ggaatagttg caaagcccgt ctctattgcg
       61 gtcttcgctg ttcttttcag cgtttgtcag agcgatgttg taatcactgt cagaatcata
      121 gttctttctt aatattgatt tcccgttctc atcaaaccat gtcagattga tggtatcata
      181 aggatagtcg cacttgcggt catggtttct gtcaaaaaca ggagcacagt catcaaatcc
      241 acacattttg tcaaaattga tgtcctcttc cttgtcacaa tccatgtttt cattcaaatc
      301 ccagcaaagt gtaacagacc gtaattgcgg ctgtgcttca cagtcggcaa ccgaacatcc
      361 cggaataccg tcaacatcct caagatcatc acagatatta tttctttttc tatcccagca
      421 tctctggcag tcaagaagat cgcaaattcc gtcttcattg atgtcttcaa taaccagtga
      481 cggattttta ggatcgtagt atcccagttc tttcaactga tctttggtgt agtagttgta
      541 ttttccgctt tcataaactt gataaagtcc tgtttctttg ttgtatgcac cgttacagta
      601 ggaagcgcct ccgctgtaat caaggtccca gcagggcgga tttgacgagg taagccccat
      661 attcctgtca aacatattcg gttccatcca gaaggggaga tatttcgcat gagtatcggt
      721 tgaatggagg ataactagtt tctgcggaat gtcaggatct attggagctc cgccgtaact
      781 gttatcaaaa catgaagtca ggaacagtgc aaaaaaaaag aaaaggaaga gtttttttga
      841 aattttcatt actcctcttt aattattttt ttacgagatt tccgataacc ttaggatgaa
      901 ctgtaggaac atcaaaatgg acatcaaaaa tgcctgtaat gcttgtgaat tctgtgccaa
      961 ccgtataagt cgaataatca ttcttgtttg taaaataagc tgtcacctga agatcatttt
     1021 caagaataaa gtttccataa gaatcaggag ctgtcttgat tttaacatct tctattttaa
     1081 caagagatcc cctgtattta gcatgttttg ccttatccat gaggtcaaca gcactgacgg
     1141 ttacagcggc aggcggagtt cctgcggagt cagcaagttt agtaacttca tttggaccga
     1201 cttcctgtcc ggcattctgt atctgatagt ggtcaaaact ggtttcataa acacctgcag
     1261 caccggttat tttaagccaa tcgccttcag cataagggaa tgtgcttcct tcgaccattt
     1321 tgtaaaccat tgctcctgaa taatcgcctg cagtacctgt gcctacaaaa aatgtccatg
     1381 tggtcgggct tccagagtgt ttgtgagcag gggatatcac catgacttta tcaactgtta
     1441 tcgcagcttt gtctgtaaca tttccctgct ggatctctgg tattgttgtt gcggtgtcgc
     1501 cggtatttcc tgtgtctccg gtgttgccgg tatctcctgt gtcgccagtg tcacctgaat
     1561 cttcaacaag gtcgtctgcg agtctgggag cgagtctgaa tgcatcataa ctataaataa
     1621 ggacgccttt tatttcagag aactttttac cttcgctcct tagaccgtcg taataataca
     1681 tcatcttgtc aatggcaagg ccgtctgtaa cttcccatcc accgtgtccg aggtcctcgt
     1741 ttgttatttc aacatcttca acctttataa aaacgctttc atatttttca ccatccgcac
     1801 cgtgatcagt agtcggggtc cattccgctt ccaccagttc gaaaggagtt gcaactgttg
     1861 aaggatccgc tataactgcc ggagtcggaa catccgccgt tccaagtttt gaaacactat
     1921 cactgtttgt ttcgatctgt gaactttcat aatattcttt ataagctcct gttacagata
     1981 ttttatcacc gatagcaaaa gcgtcaattt ctgctgtgtc tttaacaaaa acgtatattc
     2041 cggaataagg tcttgccgca gaaatcagtt cagatacata cattcccttg atgttggtgt
     2101 tattgtactg atcgtcctga ttgtagacaa taccgacaac gacgcactca aaagtcactt
     2161 ctgtttcaag agctacttca cctttctgta tctttgaaat ttcaccttca ggctctgtgt
     2221 cgcctgtgtt tccggtgttt cctgtgtcac ctgagtttcc ggtgttacct gtatttcctg
     2281 tatcaccggt gtttccggta tcacctgtgt ttcccgtgtc gccggtatca cctgtatttc
     2341 cggtattgcc tgtgttccct gtgtcgccgg tgttcccggt gtctccgtta ttgtttgtgc
     2401 ttgtcgtgtt gcagcctgca gcgattaaga acataaaaat aacgctaagt aacagtaatg
     2461 atttaatctt tttcatgatt tcctcactta agaaagatat ttaaaaaaac cgcaaaaact
     2521 caaaacatta tattgtatca cattcaatga tatttggaag ataaaaaatg aatttatttc
     2581 tgttaattct gtgtcagttg tcgaccattt tgatctttga atcggtgttt tcgatcttgc
     2641 tgtctatatt ttcacgaagc tggtcttcca ttttaagaac cgtcagtgct tcaacatcat
     2701 tgggaagatg ttttaatatc ttatgaaaat agatgtttct gtaaacgatg ttcccttctt
     2761 tggagtatat gttggcaatt attctgaaag tcctgatata gttttccata tctcccatct
     2821 gtttatatac aaacgcctgt cttttaagga gatttatgtt ctcaggatca ttggagacca
     2881 gtttttttat aaggattacg gctctttcaa actgtttcat ctcaattctg gtttcaacaa
     2941 gatctacaag gatgccggtg tcttcctttt ttagagaatg gagcatcata agacaggctt
     3001 ctttatcctt gagggtctgc tcgctgttgt ccttcagctt ttcattcaaa ggaagaagaa
     3061 tgttcaaagc atccccacac atgttttccc ggatcaaaag actgctcatg ttcagaaggt
     3121 cttttgttgt tgccttgttc atgtccttta ttcttctgaa aagatagagg gctgtcatcg
     3181 gatcattctt tctgatgaaa taatcagccg cttttctata tttgtcaggg gcatcggaaa
     3241 aacgacccat tctgatgtcc atatctgcac tgaacatatt gagaggtccg tgttcaggca
     3301 ttatcttaag accgagattt atgaagtata tcgtctgtga aggatttttc tgttcaaggt
     3361 atctgtgagc tacaccgaga tacacatcga cagctttttc cagctttttg taatatttat
     3421 acaattcagc aagctgaatg tagttttcta ttttttccgg atctttttct atttgtgaaa
     3481 gaagggatgc cgctttaaca tcaccgacga ggtctgatac atcggtgttg gcaagttttc
     3541 caagattctt caaagactct gaattgatcc cgtcctttac atttttctta agtatctttg
     3601 aaagtccgct gaaaaaacca ctgcccatac caactcctga aaacttaaac aaaacctaca
     3661 taattatatt ggaaatccaa aattagtcaa catcaaaaca caacatttgt tataatagaa
     3721 tcagcaagtt gattaataaa tttgaaactg cttaaatcgg gctggctagt ttcaattttc
     3781 agaaattgtt taaaaaaagt gttaatagga ttctgaaaat gaaatagttc ttgctcttat
     3841 gccggctcct cccggagtgt aatagacaac aaaaatcctg ttgttttcaa gctccaccgt
     3901 ccccggatat ccgcattctg caagtccgca gtcttcaatt tcgataatgt ctgaccatgt
     3961 ttcaccgtta tcaagggaat aaacaaatga aacagcttct ctttcaatat cgagttccca
     4021 gtcaagccag cggaaagaac ttattattgc tccgttattc agtttcagaa gttccggtgc
     4081 atgtgcaata aaaccgagag atctgtaacc ggaccatgtt ttacctccgt caagagaagt
     4141 gctttgcatc agatctcctt ttccgcccgg agatgatccg tatgccgttc taacctgaag
     4201 tatccagtgt agttcactga tcttaagtaa ggcaggttcc tgaagccatg tgttctcagg
     4261 agcttctgca ttgaccggct ccaagtgcca tgtttttcca tcatcttcac tgataaagaa
     4321 cactatcgga cctttcggtg tttttgacat tgaatttctg ttgagtgcat ttcctccata
     4381 tcccggaatt ataagccgtc ctgcatcttc aactatatgg ctcgaagaag cgtatgtaac
     4441 aatttcgtca ccatcgttat ccacccatat tccttcatca ttcagggaag atgcccctga
     4501 ataatcaaaa attcccggtt cgacctggac aggatcgctg aaattctttc catgatcatc
     4561 tgatgccgcg aagaacactc tgtgtatcca cggctctgtg tagtcagcag gataacggta
     4621 cttaaaccag ttcattgcga cttttccatt ttcaagaaca acaattgaag gatcgcgatc
     4681 atcaattgaa gggtcatcca tgacaacttc gggaatatca ctccatgttt caccgtcatc
     4741 cgaatagtgt gcatatattt tcccactcac ttcggcgtgt ccgctcccgg acctgtaaac
     4801 aaccatgaac tttccgttct ttaattttat gacatcaggg aaagcaaaat gttttgaagg
     4861 tgaacttatt atgtcggtat attcttcgct gatggtgaat tttatagatt tcacagtcat
     4921 ttcttcggca gcatcagcat cttcgtaaaa ttcatcagca ctatcaaagt cctgaatttc
     4981 agcatcgtcc ttctcctgtg cagattcgtt gctacatccg gtaaaaaaaa taaaaaagga
     5041 caagaaaata gaaaagatta tgtttactga cataccggcc atcctgcatt ctgaacatcg
     5101 gcgatgacat taccttccgg ggtttctttg ggtagataaa gatcgatctt gcttgttgta
     5161 aaagtgaagt cgcctgcatc tcccggaaca agattaactg aatttacggt gagggttccg
     5221 tatcctgcaa gcattacgca ctctgcttca ttcatatatt caacaacgat cagatttact
     5281 tcgtttgaat ccttgatccc gatgtttaga acggccccgt taacagttgc agttttcaaa
     5341 gatcccatta caacataggg ataagaaaac tgcgaagtgt attcatcggg cacataataa
     5401 gactgttgaa cgaactgaac tgcctgtgaa tcgtaaaaac cagccagcca gtgcacattt
     5461 gccagagaat gaggaatata gtaagtatca gtgccaagat cgatgcttgt tttgaatatc
     5521 ggctggttcc acagctcatt tccctgatta gcgccgtagt tgaattcctc aacgccattg
     5581 taaagatatt caacctcaaa agatgcggca ggcatcacag caagaggtgt tatctcgggt
     5641 tttttttcaa tgcagttcgt tgtgtcccat gaagtacagt tctctttgca ctcagccatt
     5701 ccactttcaa aatttgaatc cagctcactg cactgaacag agtttttgtc acatatttcg
     5761 ccagtttcaa ctgttccatt accgcactcg tgtcctgagc cggtgttacc tgtgttgcct
     5821 gaatcaccag tgtttccggt gtttccggta tttcccgtat caccggtatt tcctgagttg
     5881 cctgtgttcc ctgagttacc cgaatcacct gtgttgcctg aatcaccatt gctgtcaggc
     5941 agatcttcgg tatcgttttg taaattaatg tcgctgtcgt cagtaagacc cagatcatcg
     6001 tattcctcat taatgcatga agccagcgtt aacataacga taataaataa tatttttttc
     6061 atttttcctt ccccggataa aaattcctca cgggcaataa ttatgctcac agatagaaaa
     6121 tattcaaatt tcctggtcgg atttttcaag aattgtccgc atgataaaag cggctgtaat
     6181 aaaaaagccg atgctgaaaa taaaagggat gattatgtga agtttcataa gaaaataccc
     6241 gagaacaggc cagataagac cccttattcc tgtcaatgtt acatgagttg atgtcatttc
     6301 aggtatttcc atgttgtttt tcgcaaagaa catcggtcct atattccagc acagggtgac
     6361 tgccgccatt cctgctccga aagagacata tgaaagataa acagaccatt ttgcaaactc
     6421 gaccggcagg aaataagcgg ttatcataag tgcggggtga gctattagaa ttgaaaatgc
     6481 aagaactccg acatgcacag ggtctttttt gtcgaacagt ttcccgaaaa cgggtattgt
     6541 caggattatg acgactgctg aaaccactcc gcgcccgaat gaaattatgc tgtatgaaag
     6601 gttcagatgg tcaacaagca gaatcggtac tgctgcaagg acgaccataa acccgccgcc
     6661 gtatatgaaa aatgccgcct cataaacaag aaacctccgg tttttcttga aaacagcgat
     6721 cagtgtgggg aatatcagaa ggtcaacctt tctttcactc actttttctt tcatccattc
     6781 tgacaccgga acatatccga gcatgaagaa actcaggaac gaaaccaatc cgcaaatgga
     6841 gaaaactgcg atgtaaattg agctgttgtt gtccatccac aagccgaagg aatatactgc
     6901 cgcgatgaaa aagactttat tgagggatat tgagtatgca tatatttttc caagttccgt
     6961 tctggcataa ttcattttca tatagatgtt ctgaaccggt tttatgaaag tatcgccaag
     7021 atagtaaagt gctgacagga caaaaagagc ttcggcagta ttgaaaaaga agaaaagtgc
     7081 catcggaaga cgggtaaaca ttgcggcaac tgtgataatt gtctttattt ttccgggata
     7141 ttttgtgaga atgtgcgaaa aataaatact cagaagattg gtgactgagc cgagcatggt
     7201 aaacattgtt ataagaagga tcgatgcttt aagttctctt cgcataatga cttcagccat
     7261 tatgagcatt ccgtagactg ttccttcaag ggaagaatgg aacatttgta tggcaagtgt
     7321 ctttgatctg gttgttaaat tgttcatttt attagattat ttcaaggcct ggcttttgaa
     7381 tcgcgccgtt ttagtatctc tctttcaacg gtgaaaattg catcgggaac agctttttta
     7441 tttgcaaggt tttttatcca gagcggaaga ctccctgccg gatcggtata gatgtaataa
     7501 tcaaccctaa tctcatcggg ttttacctgc gtgaatttcc agtaaccctg atttattaca
     7561 actctgactt tcttttcgtt gaccgggtat ttcggatctt caaacggctc ccatgtcaca
     7621 gaaatggaac cgtcgctgtt tttcacttca catgatttca ccagataatc cctgtctgaa
     7681 gccataggtg cgtcgattac attgtatgac cagtcacact cagcagtttt tttcatctga
     7741 tcggcaaatt tgatgtattt gaagatctcg ggatgatttt tcctgtcaaa aatgacctcg
     7801 acagcttcct tgacagttcc ggtgatggtt gcgacagctt tgacctcttt aatgccggca
     7861 tctcccattt cgcggttgta aagtttaatg ttctttgaat catcgacaag aacccactcg
     7921 ggggaatttt gagcaaaaac aggaactaag ttcaaaaaca gtaaaaatag aatcacaatt
     7981 ttcatatcaa cctccaaaaa agcgttccga ttctacagtt atttcaacaa aatgtcaaaa
     8041 cgaaaacagt tttttaattt tttggttgac aaaacgactt gaaataatta tatttccgcc
     8101 tgtccgaggt ggcgaggtag ctcaggtggc tagagcagcg gactcatatc ccgcaggtcg
     8161 agtgttcaag tcactctctc gctaccacct tttttcaagt tcatagaaat ttaatttacc
     8221 ttgactgaaa ttattcgcca aagtaatata tgtagttttt a
//
LOCUS       DAOOYB010000125         8230 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_9652_length_8230_cov_7.723425, whole genome shotgun sequence.
ACCESSION   DAOOYB010000125 DAOOYB010000000
VERSION     DAOOYB010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8230
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9652_length_8230_cov_7.723425"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="PL056_08325"
     CDS             complement(<1..313)
                     /locus_tag="PL056_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47060.1"
                     /translation="MKKITLIAVVMSALLFAVACSMVKYKKGDQTKGYLNGIKKIAVQ
                     YDYSKARVGKFANETDYVADKVAEHNKKEAGKGDEWKTKWEEQKTATFATKFESLINE
                     YT"
     gene            485..2116
                     /locus_tag="PL056_08330"
     CDS             485..2116
                     /locus_tag="PL056_08330"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HPM47061.1"
                     /translation="MKKFLLLAVLIFISVSCSSKKSKLNDENSSDEDSGTGFDVENSD
                     TDIPAEEEGEEPATDSDNDGKDDEKVEADNEESDPEFNDSDTQLCRFDQCEISGICYE
                     DGEKESGHLCSVCDPQKERFSWSPLESGIICRISSGECDIAEKCDGSDFECPVDSFKP
                     KDEPCGSQTESVCDKPDTCDGYGECRANLEPENTLCTDDGNMCNGAEKCDNSGSCVSS
                     GPIVECDENEICDTGTGVCVCDADNSFFLSPDKTFCAFAGNNVPPTGQTKCYNATEEI
                     PCPTFESGLPFFGADAQYTDNTAVLTVVGESPDRVVTDSLTGLTWELDIDSVNKSWTD
                     AQNYCQNKGADWRLPTIKELTTTLNFNYSVPACDQDFCPGNSGWFWSSTIDSSDNTIV
                     KNLYMYQGDIRTNAKTDTLSVRCVKGTVFNPSGVFVSNEDPSEPVSKDSMTGLSWTKS
                     FGVATWQDALHGCEILNYGGFSDWRLPNYNEFHTVIDHSINDPASTLPNIFNSSHWTS
                     TTYIDVQGSAFSFLFSSGRSSRTAKTTSIRYLCVR"
     gene            2136..2990
                     /locus_tag="PL056_08335"
     CDS             2136..2990
                     /locus_tag="PL056_08335"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HPM47062.1"
                     /translation="MNHDIFKPLWDLMGLPYPGAYMTMLYFGFLCSFPILIVAAKKQN
                     LSVLRQLLLPVTIIPTALVFSRIFHILFEGMFFKYMDGLKENGISYLFTVMLNPFAPG
                     HVFYGGLLGGFVAGALTTMINYPKNWESFKKTADVAAVIVANGLWLARIGCFLEGCCF
                     GKPSDLFGISFPQGSRTMFLLYKIDPEYTSLYTDTQPIIPTQLVHSVSNLIICGILVK
                     LMFTGKEKNPGYVASIYLLLYPLTRFMIEFLRYDTRGSFLFLSTSQWISIIVFFAGLK
                     LYRYTKNS"
     gene            3022..5856
                     /locus_tag="PL056_08340"
     CDS             3022..5856
                     /locus_tag="PL056_08340"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HPM47063.1"
                     /translation="MKLKLCSLSIIFLLFLTLEISGSNDEKQLHHYAQRSWRSISGLS
                     HDNVTSLFRSKDGFLWAGTIEGLTRIDGSSTKVFTTRTHPGLLNNRIVGITGGNDGSI
                     YVATVTGISKISKNGKVEKVVEETDISSVAVLKNGQVFASVNNELLKISSNEIKRLSV
                     LNGLPEGNVRALTAYDNILYGGSDSGSFFSYSDGLFTNDICDINKDEVTCSSVNGSGN
                     VVFGTSKGRIFVIENSKCSPVFSESEIVYDESPVISISYINNSIRAVTGSSVILIENG
                     EKRYFRNCCNLPGKLSSVLIDEEKFMWISGDKGVTLYYDGIFSTYGKEEGLTSEMVYG
                     MVEDQNGVVWVGTRGGGLFYYSDGKFRQVPENAGIPGHFIGGLMIDDKGDLWVGTSKG
                     IVTFKPVSPFKVKMVNTARKGDVPLASAIFQDNSSRIWAGTAGGGIYLLTKDKFTLIR
                     NTGTSKEDHVAAITQDSKGVIWFATSKGLVSLEKESFKTLTVENGLPDNWILSLYADK
                     SGALFAGTMRKGLVIIMDDGRMLSLSSRKGLCSDTVFSIAKDNSDVMWFTSTHGIFSL
                     PFQSVVDSALNSEGSVRCFSFDSQDGIKRPECTGGVQPASMIRRDGSMWFPTIEGIAV
                     ASKGILAESVPHISIDSIVIDGKDKELSDVIIQKGEVSLFEIKFTASRFIHPERLNVR
                     YILNGYEDEWNDYLPLDKKTVVYQKVGAGDYRFKIEASGQNNEIKNSEISMLIESNSV
                     FSKLNAKWFFAAIILISLAAIISVNTRKRKKNKKDDVYQEVTGEIKNVDNSITEKDFD
                     SDYPDDSDVEDINDDSPKYQKSRLDENIAESYASELKSLMEEKKPHINPDLTLPELAK
                     KLNLSPNILSQVINGYCCQNFYNFVNTYRVEEVISMMKDPSMKEKSILDMAYEAGFKS
                     KTTFNTIFKKHTGVTPSEFRKNLAEKPSDL"
     gene            6055..6717
                     /locus_tag="PL056_08345"
     CDS             6055..6717
                     /locus_tag="PL056_08345"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPM47064.1"
                     /translation="MKFKAFTLIELMVTLGVITIIATASVPQVQMWIARNKGIQIVSQ
                     VISDFSKAKSIARSIVKQQDAVVAGSFVFGSRPQVSMYFKADSYYILQRNSMTPGGWS
                     ETSDTVLKSFRLPSKVELYSVNGSLVTSGITVLFSSSGRLKKDDGSFITPVKDKTYIE
                     DCGSEKSDLKGVGVFMAVFKVDASNNKTLWYRTEISPAGDFFACFSTENSFLENANII
                     EL"
     gene            6734..7291
                     /locus_tag="PL056_08350"
     CDS             6734..7291
                     /locus_tag="PL056_08350"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPM47065.1"
                     /translation="MKELLKGFTLLEVMVSIFIFSSGMLAYMAYHSKVNSMVFDNESS
                     KIAHSLALNLAEEMMSMSSEDYYELSKDKENGNSIWMDNILFDSDENMAGPFDSRGKP
                     GSSGDKFQFYRFIRFSTYDIETDTYNPAPSLYGKLRHFDIIVAWPFKQYGEHTNKCGD
                     SNYITSNSNIYCNFLSIPVVRFAEY"
     gene            7305..>8230
                     /locus_tag="PL056_08355"
     CDS             7305..>8230
                     /locus_tag="PL056_08355"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPM47066.1"
                     /translation="MKNIRKLHFIGFTLIELMVSMFLTLIVIGTFYKVYNNSLKSEKR
                     TSSKTSVVVTGERIINTIADPLKLLGLANVFEVDGGGDWLANSIIVDSDGAANSGDVT
                     FGFRSPYGGFVSKLMNTASGDVGSGGSCEFKISYTTSLSSEANQKVNLFSSNGIYSAT
                     LNSVDWEQSQLKINDLKDSNGDAISGSCGAFFPLGSLITGPNYYYKISYLETNGGSIK
                     LERYNDLADTSPQTVLDFKSVDNSYSIPLFILEFLREYEDTSTGVLEKVKEWVESPTA
                     DELRDVKAVRVGFVLLADKDRNVNTGTGLSAI"
BASE COUNT         2526 a         1419 c         1774 g         2511 t
ORIGIN      
        1 ttgtatattc attgataaga gattcaaact tagttgcgaa agttgctgtt ttctgctctt
       61 cccattttgt tttccattcg tcgccttttc cagcttcttt tttgttgtgc tcagctactt
      121 tgtctgcaac ataatctgtt tcatttgcaa atttaccaac tcttgctttt gaataatcat
      181 actgtacagc gattttttta ataccgttga gataaccctt tgtctggtct ccctttttgt
      241 acttaaccat ggaacatgca actgcaaaaa gcagtgcaga cataacaact gcgataagtg
      301 tgatcttttt cattttttac tccttaaagt aagttaataa gaacgacaag tgagaaaata
      361 gggtttcttt cattaaagtc aagaaatagt ggttgttttt ctgaaaatga ctttacacag
      421 gcaaaaaaaa caataatatt gagcagcatg ttttaattct gatatcactt gaggggggcg
      481 gttcatgaaa aagtttcttt tgctggcggt cttaattttt atttcggtct catgttcatc
      541 aaaaaaatca aaattgaatg atgagaacag ttctgatgag gattccggaa caggttttga
      601 tgttgaaaat tccgatactg acatacctgc cgaagaagaa ggtgaagagc cggcaacgga
      661 ctcagataat gacggaaaag atgatgagaa agttgaagcc gataatgaag aaagcgatcc
      721 tgagtttaat gattcagaca ctcagttatg caggtttgat caatgtgaga tctcagggat
      781 ctgctatgaa gatggagaaa aagaatcggg acatctttgt tcagtttgtg atcctcaaaa
      841 ggagagattt tcatggagtc ctttggaatc agggatcatt tgcagaatca gttcaggaga
      901 atgcgacata gctgaaaaat gtgacggatc agattttgaa tgtcctgttg actcatttaa
      961 gcccaaggat gaaccctgcg gaagtcagac tgaatcggtt tgtgacaagc cggatacatg
     1021 cgacggatat ggagaatgca gggctaatct tgaacccgaa aatacattgt gcactgatga
     1081 tggaaatatg tgtaacggcg ctgaaaaatg tgataattca ggaagttgtg tttcttccgg
     1141 cccaattgtt gaatgtgatg agaacgaaat ctgcgatacc ggaacaggag tgtgtgtctg
     1201 tgatgcagat aacagttttt ttctttcacc tgacaagaca ttctgcgctt ttgcagggaa
     1261 caatgtccct cctacaggtc agacaaaatg ttataatgcc actgaagaga ttccgtgccc
     1321 gacttttgaa agcggactgc ctttcttcgg tgctgatgcg caatatactg ataatactgc
     1381 ggttctgact gttgtcggtg aatctcctga cagagttgta acggattcat tgaccggtct
     1441 tacctgggaa cttgatatcg attctgtcaa taaaagctgg acagatgcgc agaattactg
     1501 ccagaacaaa ggtgctgact ggagactgcc gacaataaaa gagctcacaa cgaccttgaa
     1561 tttcaattac tcagttcctg cctgtgatca ggatttctgt ccgggtaatt cagggtggtt
     1621 ctggtcatca acgatagatt cttcagacaa tacaattgta aagaatctct atatgtatca
     1681 gggagatata agaactaatg caaaaacgga cactctttct gtccgttgtg tgaaaggaac
     1741 ggtctttaac ccttcaggag tttttgtcag taatgaagat ccttcagaac ctgtgtcaaa
     1801 agacagtatg accggtcttt cctggacaaa aagtttcgga gttgcgacat ggcaggacgc
     1861 tctgcacggt tgcgagatat tgaactatgg aggtttttct gactggagac tgcctaatta
     1921 caatgaattt catacagtta tagaccattc cataaatgat ccggcatcaa ctcttccgaa
     1981 catatttaat tcatcacact ggacatcgac tacatatatc gatgttcagg gatccgcttt
     2041 ctcatttctt ttttcaagcg gaagatcttc aagaacagca aaaacgactt caataagata
     2101 tctttgcgtt agataggttt taagtaggag gatatatgaa tcacgacatt ttcaagccgt
     2161 tatgggacct catggggctt ccctatcccg gcgcatatat gacaatgctt tatttcgggt
     2221 ttttatgttc atttcccata ttaattgtag cggctaaaaa acaaaacctt tctgttttga
     2281 gacagctgct ccttccagtg acgattattc ctacggcgtt ggtgttttca agaatattcc
     2341 acattctttt tgaagggatg ttctttaaat atatggacgg tttaaaggaa aacggtataa
     2401 gttatctttt tacagtaatg ctcaaccctt ttgctcccgg acatgttttt tatgggggac
     2461 ttctgggcgg ttttgttgcc ggagcactga caacaatgat taactatcct aagaactggg
     2521 aatcttttaa aaaaactgcc gatgttgctg cagtaattgt tgcaaacggt ctctggcttg
     2581 ccaggatcgg atgttttctt gaaggttgct gtttcggcaa accttcagat cttttcggaa
     2641 taagtttccc tcagggctca agaaccatgt tcctgcttta caaaattgac cctgaataca
     2701 caagtcttta tacggatacg caaccgataa taccaaccca gctcgtccat tctgtttcca
     2761 acctcatcat ttgcggaata cttgtaaaac tgatgttcac cggaaaagaa aagaaccccg
     2821 gatatgttgc ttcaatttat cttcttcttt atcctctgac aagattcatg atagaattct
     2881 taagatatga cacccgtgga agttttctat ttctctcaac aagtcagtgg atatccatca
     2941 ttgtcttttt tgctgggctt aagctttaca gatataccaa aaattcttga taaaaaagag
     3001 ttattgaaat acaattccgc catgaaattg aaactatgct cgttatccat aatatttttg
     3061 ctgtttttaa cgcttgaaat cagcggctca aatgatgaaa aacagttgca tcattatgct
     3121 cagagaagct ggagatccat atcaggtctg agtcacgaca atgtcacatc acttttccgc
     3181 agcaaagacg gtttcctctg ggcaggcacc attgaagggt tgacaaggat agacggttcc
     3241 tctacaaaag ttttcacgac aagaacacat cccggacttc tgaacaacag gattgtcgga
     3301 ataacgggag gcaatgacgg atcgatatat gttgcaacag tcacgggtat ttcaaagatc
     3361 tcaaagaacg ggaaagtcga aaaagtggtt gaagagacag atatttcatc tgttgcagtt
     3421 ttgaaaaacg gtcaggtctt tgcatctgta aataatgaac tgctgaaaat ttcatctaat
     3481 gaaattaaga ggttgagcgt tcttaacggt ttgcctgaag gcaatgtaag ggcgttgaca
     3541 gcttatgata atattcttta cggaggaagt gattcaggaa gtttcttttc gtattccgac
     3601 ggtcttttca ccaatgatat ctgtgacata aataaagatg aagtgacttg ctcatctgta
     3661 aacggaagcg gaaatgtcgt tttcggaaca tcaaaaggga gaatatttgt aatagaaaac
     3721 tcaaaatgtt cccccgtttt ttctgaaagt gaaattgttt atgatgaaag tccagttatt
     3781 tcaatatcat acatcaacaa ctccatcagg gcagttacag gaagctcagt cattttaatt
     3841 gagaacggtg aaaaaagata tttcaggaac tgctgtaatc tgcccggaaa actttcctca
     3901 gttctcattg atgaagaaaa attcatgtgg atatcaggag acaaaggtgt aactctttat
     3961 tatgacggga tattctcaac atacggcaaa gaagagggac tcacttccga aatggtttac
     4021 ggcatggttg aggatcaaaa cggagttgtc tgggtcggaa caagaggagg cgggcttttt
     4081 tactattctg acgggaaatt caggcaagtt cccgagaatg caggaattcc cggtcatttt
     4141 atcggcggat tgatgattga tgataagggt gacttatggg tcggaacttc aaaagggatt
     4201 gttaccttca agccggtttc tccctttaaa gttaaaatgg tgaatactgc aagaaagggt
     4261 gatgttccgc tggcatcggc aatttttcag gataattcca gccgcatctg ggcaggaacg
     4321 gcaggcggag ggatatatct tttaacgaaa gacaagttca cactgatcag gaacaccggc
     4381 acttcaaaag aagatcatgt cgctgcaatt actcaggatt caaagggagt tatctggttt
     4441 gcgacttcca aaggacttgt ttcactggag aaagaatctt tcaaaacatt aacagttgag
     4501 aacggacttc ccgacaactg gatactctca ctttatgctg ataagtcggg ggctttgttc
     4561 gcaggaacga tgagaaaagg acttgtgata ataatggatg acggaaggat gctgtcgctg
     4621 agttcaagaa agggtctttg cagtgataca gttttttcca tagcgaaaga taacagtgat
     4681 gtaatgtggt tcacgagcac tcatggaata ttttcccttc catttcaatc agttgtggat
     4741 tcggcactca acagtgaagg gtcggtcaga tgcttttcat ttgactcaca ggacgggata
     4801 aaaagacctg aatgcaccgg aggagttcag cccgcttcaa tgataagaag ggacggctca
     4861 atgtggttcc cgacgattga agggatagct gttgcttcaa aagggattct ggctgaaagt
     4921 gttcctcata tttcaataga cagtattgta attgatggaa aagacaagga gttgtcagat
     4981 gttataatac aaaaaggcga agtgtccctt tttgagataa aattcacagc aagcaggttc
     5041 attcaccctg aacgattgaa cgtcagatat attctgaatg gttatgaaga tgaatggaat
     5101 gattatctgc cgcttgataa aaagacagtt gtttatcaga aagttggcgc cggtgattac
     5161 agattcaaga tcgaagcatc aggacagaat aatgaaataa aaaacagcga aatatccatg
     5221 ctgatagaaa gtaatagtgt tttcagcaag ttgaatgcaa aatggttttt cgctgcaatt
     5281 attctgatct ctcttgccgc gatcatctct gtaaacacca gaaaaaggaa aaagaataaa
     5341 aaagatgatg tttatcagga agttacaggc gaaataaaaa atgttgacaa ctctataact
     5401 gaaaaagatt tcgattcgga ttatccggat gacagcgatg ttgaagacat caatgatgat
     5461 tctccgaaat atcagaagag ccgccttgat gaaaatatcg ctgaaagtta tgcatctgag
     5521 ctgaaatctc tcatggaaga gaaaaaaccg cacataaatc ccgacctcac attgcctgaa
     5581 cttgctaaaa agcttaatct ttctcctaac atattgtctc aggtcattaa cggttactgc
     5641 tgtcagaatt tctataattt cgttaatact taccgggttg aggaagtcat ttccatgatg
     5701 aaagacccgt caatgaagga aaaaagcatt cttgacatgg cttatgaagc aggattcaaa
     5761 tcgaagacca ccttcaacac catttttaaa aagcatacag gtgtcacacc gagtgaattc
     5821 agaaagaatc tggctgaaaa gccctcagat ctataaaatc acatatttca aaaaatattt
     5881 tttaagatat ttttaaaaac atttaagatg ccgtaattat tttgttttaa tagtccgtcc
     5941 ctatccaggc gaactttttt gggaatggtg ttctgtctag ttctatacgg tcggacgact
     6001 tagtaatatg tatataataa actcgaaatt gtgaccatga cggagggatc ggatatgaag
     6061 ttcaaggcat ttacattgat cgagctgatg gtgacgcttg gagtaataac aatcattgca
     6121 acagcaagtg tgcctcaggt gcagatgtgg attgcaagaa acaaaggaat tcaaatcgtt
     6181 tctcaagtta tttcagattt ttctaaagct aagtcaatag caagaagtat tgttaaacaa
     6241 caagatgctg tagttgcagg aagttttgtt tttggatcac gacctcaagt ttcaatgtat
     6301 tttaaagcag acagttatta tattttgcaa agaaacagca tgactcctgg tggttggtct
     6361 gaaacaagcg atactgtcct taaatctttt cgattgccat caaaagtaga gctttattct
     6421 gtgaacgggt ctttagttac atcgggaata accgtacttt tttcatcatc tggacgactt
     6481 aaaaaagatg atggttcttt tattactcct gttaaagata agacctatat tgaagattgc
     6541 ggaagtgaaa aatccgattt aaaaggagta ggtgtgttta tggcggtttt taaggtggat
     6601 gcttcaaata ataaaacttt atggtataga actgagatat ctcctgctgg agattttttt
     6661 gcatgttttt ctacggaaaa cagttttttg gagaacgcaa atattattga gttataaaaa
     6721 ataggtttgt atgatgaaag agttattaaa aggttttact ttgcttgagg ttatggtttc
     6781 aatattcata ttttcttcag gtatgcttgc atatatggcg tatcattcaa aagtaaattc
     6841 aatggtgttt gacaatgagt cttcaaaaat agctcattcg cttgcattaa accttgcaga
     6901 ggaaatgatg tcgatgtcat ctgaagatta ctatgagttg tcaaaagaca aagagaacgg
     6961 taattctatt tggatggata atattctttt tgatagtgat gaaaatatgg cgggaccttt
     7021 tgattctcgt ggcaaacctg gatcttctgg tgataaattt caattttata gatttatcag
     7081 gttcagtaca tatgatattg aaactgatac atataatcct gcgccgtcgc tttatgggaa
     7141 gttaagacat tttgacatta ttgtcgcatg gccttttaag caatatggag aacatacaaa
     7201 taaatgtggt gattcaaatt atattacttc gaacagcaac atttattgca attttctttc
     7261 tattcctgtt gttcggttcg cagaatattg attaggaggg gcgcttgaag aatattagaa
     7321 aattacattt tatcggtttt actttgatag aattaatggt ttccatgttc cttactctta
     7381 ttgttatcgg aacattttat aaggtataca acaattcttt aaagtctgaa aagaggacca
     7441 gttcaaaaac atctgtggta gtgacaggag aaaggattat caatacaata gctgatcctt
     7501 taaaattact tggcttggca aatgtttttg aggtagatgg cggtggtgac tggcttgcaa
     7561 attcaattat tgttgattcg gatggagctg caaattcggg agatgtcaca tttggattca
     7621 ggtcaccata cggagggttc gtatccaaat taatgaacac agcttctggg gatgttggaa
     7681 gtggagggtc atgtgaattt aaaatttcat atacgacatc tctttcatca gaagccaatc
     7741 agaaagtcaa tttgttttca tctaatggta tttatagtgc aactttgaat agtgttgact
     7801 gggagcaatc tcaattgaaa ataaatgatt taaaagatag caacggggat gcgataagtg
     7861 gttcctgcgg tgcttttttc ccacttggat cgttgatcac aggacctaac tattattaca
     7921 agatctctta tcttgaaacc aacggcggaa gtattaaact tgagagatat aatgatcttg
     7981 ccgatacttc tccgcaaact gttcttgatt ttaaatcggt tgacaacagt tattccatac
     8041 ctttattcat tttggaattt cttagagaat acgaagatac ttcaaccggt gttttagaaa
     8101 aggttaagga atgggtggaa tcaccaacag cagatgaatt gcgcgatgta aaagctgtaa
     8161 gggtgggctt tgtgctcctt gcggataaag acaggaatgt gaatacagga acgggtctat
     8221 cagcaattga
//
LOCUS       DAOOYB010000126         8079 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_9913_length_8079_cov_10.647059, whole genome shotgun sequence.
ACCESSION   DAOOYB010000126 DAOOYB010000000
VERSION     DAOOYB010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8079
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9913_length_8079_cov_10.647059"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            85..1776
                     /gene="ftcD"
                     /locus_tag="PL056_08360"
     CDS             85..1776
                     /gene="ftcD"
                     /locus_tag="PL056_08360"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HPM47067.1"
                     /translation="MSKIIECVPNFSEGRDLSVIDKITAEIKKVKGVKLLDVDPGYDT
                     NRTVVTMVGEPEAVMEAAFQAVKKSHELIDMRKHKGAHPRFGACDVCPIIPVSGVTMD
                     DCVGYAHKLGKRLGEELDFPVYFYEYAATSKERKNLAKVRAGEYEGLEAKLKDPQWLP
                     DFGPAEFKPAKGATAVSARDFLIAYNLNLNTTNAKKANFIALRLREQGYPKKDENDKP
                     LLDESGKPVMNPGLFKNCKAIGWYVDDFKRAQISINLTNYHVTNMHHVFDAASKLAEE
                     KGLRITGSEIVGVVPKEALIQAGIHYLEKADSCAGQSEAELIKIAEQSLGLSDVVKFN
                     PEEKIIEYMVKESKGLVDKTVTEFNDLLASDAPAPGGGSVAALCGSLAASLGSMVANL
                     TYNKKGYKQYNTEMNEIAKNMQVRKGELLHLIDEDTNAFNIMMDAMKAQNAAKKGGDA
                     TEIGKADARMYEANKYAISIPLKVLEKTFELLPALKAVGERGNSNAVSDAGVGALLVE
                     SGVKGAALNVKINLMNFKKEDPFHIETVSRMNSILSTLDAKVNEILEIVEKKIAG"
     gene            complement(1783..3264)
                     /locus_tag="PL056_08365"
     CDS             complement(1783..3264)
                     /locus_tag="PL056_08365"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HPM47068.1"
                     /translation="MEILLISPPVFDFYSTSHRMEPLGLQYIKGALLNFGHRVELYDS
                     TASGKVKHKKMPNELEYLLKYYEEDTSPFSLHSGYKRFGDSFSRIIKFINDGNFDIIG
                     ISSLFSAYHPDVEDLVKEIKKYSKIPVIIGGTAVTAQKEVLFENSSADFMISGCGTES
                     MPLFADFIAGKLDIEKVPGLIYRKNGNILSNAPSTEPAWSKDIIPERENLRIFRKKKI
                     AKTVLSSGCRNRCSFCSIHRDNKFALREITHIRKEFEYLKNIGTEIIDIEDDDIFSDP
                     HFTEEVLKLLKEFHGSGIQFTAMNGLTAKNILPIADGLIEAGFLKLDLSLVTSSEKAI
                     SGMKRPHDLITIEKIVEVVKTRIEVEVFLIPGLPGTTLENTLETMIHLNKLKIKCGLS
                     PLYLVPGVPMFESMGIPENLRLCRGSALYPFDSPERSNIASLLKISRFLNYSLTSASD
                     PDFEENRNYFLKSLERKKWYKRTKTGEWTDSFEFTGNFSGIKM"
     gene            3405..4691
                     /locus_tag="PL056_08370"
     CDS             3405..4691
                     /locus_tag="PL056_08370"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HPM47069.1"
                     /translation="MEFGKYIEIFNHEVVPALGCTEPIAVALAVATSTKHLGKPVERA
                     EIFLSGNIMKNGVGVGIPGTGMVGLHIAAALGITGGDCSKELEVLNSVTKDQIIAAKE
                     LVKKGVIKISIKEKVDKLYIEATCFNGSNQARTLICGSHTNIVLAEVDGNVIFQKTCG
                     AFVAKDESIPGENVEHHMTVEGIWKFINEVPLKDIEYVLKGVEMNRTIGLEGLTGNYG
                     LRVGKTLRSNIVKGLLCDDFSNWAMALTAAASDARMAGSTLSVMSNSGSGNQGIAVML
                     PILAASERLHCSKEQLVRALYLGNLIAIHLKEFMFRLSALCGVVTAATGAACGVVYLL
                     GGDLPQVNSAIKNMIGNISGMICDGAKAGCALKVSTAVSAALQSAFLAIENIEISEFD
                     GIIEKDIEKTIRNLAMIASEGMETTDKVILDIMSCK"
     gene            4699..6027
                     /locus_tag="PL056_08375"
     CDS             4699..6027
                     /locus_tag="PL056_08375"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HPM47070.1"
                     /translation="MKRRYYNWYSKTYIFNEKLNRKFTTAGAAVFISAFTLLFFGLNT
                     NLSMLFVTFSIAVSLIITDALSLSGKMRQLKVVRYLPPFISRGQKLKYKVLIKNYGGE
                     LQKGELFYKEMISDPRPDYESFISTPEPGEEKRNRYDRKMAYYRWKWLIGRNSGGGTQ
                     EFSVTGEKMDDGEVFEAEFMPERRGKIMFSGAYLFRKGVFGLFKKGKVVDFPGDIIVF
                     PKILHVDQMECMDGSRNADSEKVRETPETGPGYELKSLREYVPGDSPRTIHWRSSAKT
                     GDLKVKEFHKEVDAGTVMFIDNFFSESYSDDFEDLMTVAASLLNNSYEVGKMPQSIVI
                     GQESFDIASTSRDSLLRALSALALTGNDRSANLINSVTDLTESAKECCSVIFLTSNYD
                     EKRFLMIESLFKHKVPVTLIFTGERKTLSAPVVFEKHVSKEDLNSGALKL"
     gene            6024..7964
                     /locus_tag="PL056_08380"
     CDS             6024..7964
                     /locus_tag="PL056_08380"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HPM47071.1"
                     /translation="MRFPPFFIGAATIFWGFCAGWEYFSIVAALVFESSMIVKTRFDL
                     KSADFIRISDLSSIVMLILLLYSYLENEPRMIFLYFITTTPIVFMPLLFAQLYSTSDK
                     VVIGTKFGKNIHAHAPVDIRPLYLLMVFFATAGSDVRTVWFFAGTFILVSILFSESIR
                     DFRSFLRYFMFSAVSIAITLSIAFSIVLAHKAIREKMMEMYRNWYNQQSSDPFKTSTA
                     MGETGYLKLSGNIVMRVMPEKMEGIPLYLKSSDYNILTGNVWHSRSKDSMPVFPDGEM
                     EWQLFGEGEGKRKASLSVWMGKNGKGVLSLPIGSKRALKMDVAGIEKTGLGSILVEEG
                     PDLLEFVMTYDPQFRYEPMPEEKDLMIPDSEKEVIAKVMSDNLLKGETNFQTLENIER
                     YFMNFSYTLQLEPSSGRSVLDDFLNRTKAGHCEYFATATTLMLRSAGIAARYSTGYSV
                     EEYSTLERSFIVRARDAHAWVTAFVGGKWITVDTTPSQWRSLDRESKSFIEPVRDLLS
                     WLRLQYENFRRQKNVEFNRALIVIATVLTLFMMIKIYIRRKRVSGTELKSDRFSGKVD
                     GLDSPFYQVLIKCSSEGITKNDNETLRKWVIKNSEKLKDSKDIMKMIHLHEQLRFDPA
                     SPKNDIRNELQKYCDKWIAGES"
BASE COUNT         2391 a         1484 c         1924 g         2280 t
ORIGIN      
        1 cctacgaaaa tcttgacact tttttgtttg atttatactc tcttgcggaa attgagtttt
       61 ttattttttt tataggagcg ggtgatgtca aaaattattg aatgtgttcc gaatttttca
      121 gagggaagag atttgagcgt gattgataag atcactgctg aaattaaaaa agttaagggg
      181 gttaagctcc ttgatgttga tccgggatac gatacgaaca ggactgttgt gactatggtg
      241 ggtgagccgg aagcggttat ggaagcggca tttcaggcgg taaagaaaag tcacgaactt
      301 attgacatga gaaagcataa gggggcgcat ccgaggtttg gagcgtgtga tgtctgtcct
      361 ataattccag ttagcggtgt gacgatggat gactgtgtcg gttatgctca caaacttgga
      421 aaacggcttg gcgaagagct tgatttccct gtgtattttt atgaatatgc ggcaacaagt
      481 aaagaaagaa agaacctcgc aaaagtgaga gctggtgaat atgaagggct tgaagcgaaa
      541 cttaaagatc cgcagtggtt gcccgatttc ggtcctgctg aatttaagcc ggcaaaaggg
      601 gcgacagcgg tttcagcaag ggattttctt attgcttata atttgaatct caatacgacc
      661 aatgcaaaaa aagctaattt catagctctc cgtcttcgtg aacagggata tccgaaaaaa
      721 gatgaaaacg ataagccgct tcttgatgaa tcagggaaac cggtaatgaa tccgggtctt
      781 tttaagaact gcaaggcgat cggatggtat gttgatgatt tcaaaagagc gcagatatca
      841 ataaatctta ccaactatca tgtgacaaat atgcatcatg tttttgatgc cgcttcaaaa
      901 cttgctgaag agaaaggact tcgtataaca ggcagtgaaa ttgtgggtgt agttccgaaa
      961 gaagcgctta ttcaggcagg aattcattat cttgaaaaag ccgatagctg tgcaggtcaa
     1021 agtgaagccg aactgatcaa gatcgctgaa cagtctcttg gacttagcga tgttgtaaag
     1081 ttcaatcccg aagagaagat aattgagtat atggtgaaag agagcaaggg gcttgttgat
     1141 aagaccgtga cagaattcaa cgatcttctt gcaagtgatg ctccggctcc tggcggcgga
     1201 tctgttgccg cactttgcgg atctcttgcc gcatctcttg gaagtatggt cgccaacctc
     1261 acatacaaca aaaaagggta taagcagtat aatacagaaa tgaatgaaat tgctaagaat
     1321 atgcaggtga gaaaagggga actcctccat ttaattgatg aagataccaa tgctttcaac
     1381 ataatgatgg atgcgatgaa agctcagaat gccgctaaaa aagggggaga tgcgacagaa
     1441 attggaaaag ccgatgccag aatgtatgaa gcaaataaat atgcgatctc aattcctttg
     1501 aaagtgcttg aaaaaacctt tgaactgctt ccggcactca aagcggtcgg agaaagaggg
     1561 aattcaaatg cagtcagtga tgcgggagta ggtgcactgc tggttgaatc gggagtcaaa
     1621 ggggccgctc tcaatgtgaa aataaatctc atgaacttca aaaaagaaga tccgttccac
     1681 atcgaaacag tcagcagaat gaattcaatc ctttcaactc ttgatgcaaa agtcaacgag
     1741 atccttgaga tcgttgagaa aaagatagcc gggtaggtta aatcacattt ttattccaga
     1801 aaaattccct gtaaattcaa aggaatctgt ccattcaccg gtttttgtcc gtttatacca
     1861 ttttttccgt tcaagtgatt ttagaaaata gttacggttt tcttcaaagt cgggatctga
     1921 tgctgatgta agtgaataat tcaggaatct tgagatcttt aataatgaag cgatattgct
     1981 tcgttcaggg ctgtcaaacg gatagagtgc gcttccgcgg cagagccgga gattttcagg
     2041 gattcccatg ctttcaaaca ttggaactcc gggaacaaga taaagggggc tgagtccgca
     2101 tttaattttg agcttgttca gatggatcat ggtctcaagt gtgttttcaa gagttgttcc
     2161 ggggagaccg ggaatgagaa aaacctcaac ttctattctg gttttaacaa cttcaactat
     2221 tttttcgatc gttataagat cgtgaggtct tttcatgccc gatattgctt tttctgaact
     2281 ggtgacaaga gaaaggtcaa gctttaaaaa cccggcttca ataagtccat ctgcaattgg
     2341 gagaatgttt ttggcagtaa gtccgttcat ggcagtgaac tgaattcctg atccatgaaa
     2401 ttccttaagt aatttaagaa cttcttctgt aaagtgaggg tctgaaaaga tgtcgtcatc
     2461 ttcaatatct attatctctg ttccgatatt tttcagatat tcaaactctt ttctgatgtg
     2521 ggtgatctct cttaaagcga acttgttgtc gcggtgaatt gagcagaacg agcacctgtt
     2581 cctgcagccg gatgaaagaa cggttttagc gatctttttt ttgcggaaga tccttaaatt
     2641 ctctctttcg ggaatgatat ctttgctcca tgccggctct gttgaaggag catttgacaa
     2701 aatattccca tttttgcgat atatcagccc gggaactttt tcaatgtcga gttttcctgc
     2761 aatgaaatct gcaaaaagag gcattgattc tgtgccgcag cccgaaatca tgaaatcagc
     2821 cgatgagttt tcaaacagca cttccttttg agcagttact gccgttccgc cgatgatgac
     2881 agggatttta gaatattttt ttatctcttt gacaagatct tcgacatcgg gatgataagc
     2941 cgaaaaaaga gaactgatgc cgataatatc aaagtttcca tcgttaatga atttgatgat
     3001 cctgctgaaa ctgtcaccga atcttttata tccgctgtga agggaaaaag gggatgtgtc
     3061 ttcttcataa tatttgagca ggtattcaag ttcattgggc atttttttgt gcttaacctt
     3121 gcctgaagcg gttgaatcat aaagttcaac ccggtgtcca aagtttaaga gagctccttt
     3181 tatatattgg aggccaagcg gctccattct gtgggaagtt gaataaaaat caaataccgg
     3241 aggcgaaata agcagaattt ccattatgca accatctaaa atgtccatgc actttatacc
     3301 agaatcggtt gaaatggcaa ctgggaaaga gggcgtgatc gaatgaattt gtcttttggt
     3361 actcttttat tagaatgaag agacatttgt aaggaggagt ttccatggag tttggtaaat
     3421 acattgagat ctttaatcac gaagtcgttc cggcgctcgg ctgcactgaa ccgattgcag
     3481 ttgccctcgc agtggcaact tccacaaaac atctgggaaa accggttgaa agggcggaga
     3541 tcttcttaag cggaaatata atgaagaacg gcgtcggtgt cggaattccg gggaccggta
     3601 tggtcgggct tcatatcgca gcggccctgg ggataaccgg cggtgactgt tcaaaagagc
     3661 tcgaagtttt aaacagtgtc acaaaagatc agattatcgc ggcaaaagag cttgttaaaa
     3721 aaggagtgat aaagatatcc ataaaagaaa aagtggacaa actttatatc gaagcgactt
     3781 gcttcaatgg ttcaaatcag gcccggacgc taatctgcgg aagccatacc aacatcgtgc
     3841 ttgctgaagt tgacgggaat gtgatatttc agaaaacatg cggcgctttt gtggcaaaag
     3901 atgaaagcat tccgggagag aatgttgaac accacatgac agtcgaagga atatggaaat
     3961 ttataaatga agtccctttg aaagatatcg aatatgttct taaaggggtc gaaatgaaca
     4021 ggacaatcgg acttgagggg cttacaggca actatggtct gagggtcgga aaaaccttga
     4081 ggagcaatat tgttaaaggg ctgctgtgtg atgatttttc caattgggct atggctctta
     4141 ctgccgctgc aagtgacgcc agaatggcag ggagcaccct ttctgtaatg agcaattcag
     4201 gaagcggaaa tcagggaatt gcggtcatgc tgcccatcct tgccgcctct gaacggcttc
     4261 actgctcaaa agaacagctt gtcagagctt tgtatcttgg gaatctgata gcaattcatc
     4321 tcaaggagtt tatgttccgg ctttcagctc tttgcggagt tgttacagcg gcaacaggtg
     4381 cggcctgcgg agtcgtatat cttctcggag gggatcttcc tcaggtgaat tccgcgataa
     4441 aaaatatgat cggcaacatt tcagggatga tctgtgacgg ggccaaagcc ggttgtgccc
     4501 ttaaagtttc aacagcggta agtgccgcac tccaaagtgc ttttcttgca attgaaaata
     4561 ttgagatatc tgaatttgac ggaataatcg aaaaagacat cgaaaaaacg atcagaaatc
     4621 ttgccatgat agcgtcggaa gggatggaga ctacggacaa agtgatcctt gatatcatga
     4681 gctgtaaata gagaacagat gaagcgtcgt tactacaact ggtatagcaa aacttatatt
     4741 ttcaatgaaa aactgaatag aaagttcact acggcgggag cggcggtttt catttctgca
     4801 ttcacgcttc tttttttcgg gctgaacact aatctttcca tgctttttgt gacattttcc
     4861 attgcggttt ctcttataat tacagatgct ttgtcgcttt ccgggaagat gcgtcagctc
     4921 aaagtcgtcc gttatcttcc tccgttcata tcaaggggac agaagttgaa atacaaggtt
     4981 ctgataaaaa attacggagg agagcttcaa aagggagagc ttttctacaa ggagatgatc
     5041 tcagatccaa ggcctgatta cgaatcgttt atttcaactc ctgaaccggg agaggaaaaa
     5101 aggaaccggt atgacagaaa aatggcttat taccgctgga aatggcttat cggaagaaac
     5161 agcggcggcg gcacacagga gttttctgta acaggagaaa agatggatga cggagaggtt
     5221 tttgaagctg aattcatgcc ggaaagaaga ggaaagataa tgttttcagg ggcatatctt
     5281 ttcagaaagg gggttttcgg cctgttcaaa aagggaaagg tcgttgactt tccgggcgat
     5341 atcatcgttt ttccaaagat actgcatgtt gatcagatgg aatgtatgga cggatcgcga
     5401 aatgccgaca gcgaaaaggt tagggagact cctgaaaccg gaccgggata tgagcttaaa
     5461 tcgctcaggg agtatgtgcc gggagactca ccgagaacaa ttcactggag atcgagtgca
     5521 aagaccgggg atctgaaggt aaaggagttc cacaaggagg ttgacgcagg gacggtgatg
     5581 ttcattgata actttttttc tgaaagttat tcagatgatt ttgaggatct tatgactgtt
     5641 gccgcctcac ttcttaacaa ttcgtatgaa gtcggtaaaa tgcctcagtc aattgtgatc
     5701 ggtcaggaat cgtttgatat tgcttcaacc tcaagagatt cgcttttgag agctctttca
     5761 gcccttgctc tgacaggaaa tgacagatct gcaaatctta tcaactccgt aacggactta
     5821 actgaatcgg caaaggagtg ctgttctgtc atatttctta cttcaaatta tgacgaaaaa
     5881 agatttctga tgattgaatc gcttttcaaa cataaagttc ctgtaacttt gatatttaca
     5941 ggtgaaagaa aaacgctgtc ggctccagtc gtatttgaga agcatgtttc aaaggaagat
     6001 cttaattcgg gggcacttaa attatgagat ttcctccgtt tttcataggt gcggcgacaa
     6061 ttttctgggg attttgcgcc ggatgggaat acttctcgat tgttgcggcg cttgtttttg
     6121 aatcttccat gatcgtaaaa acaaggtttg atctgaaatc tgctgatttc ataaggatat
     6181 ccgatctcag ttcgattgtc atgctcatcc ttctcctcta ttcgtatctt gaaaacgagc
     6241 cgaggatgat atttctatat tttataacga caacaccgat agttttcatg ccgttacttt
     6301 ttgcccagct ttattcgaca agtgacaaag tggttattgg cacgaaattc ggaaagaaca
     6361 tccatgccca tgctccggtt gacataaggc cgctgtatct tctgatggtg ttctttgcaa
     6421 ctgccggttc agatgtgaga actgtctggt tctttgcggg aactttcatc ctcgtttcta
     6481 ttcttttttc tgaaagcatc agagatttca gatctttttt aagatatttc atgttttccg
     6541 cagtttcaat agcgataacg ctttcgatag ctttcagcat agttcttgcc cacaaagcta
     6601 tcagagaaaa aatgatggag atgtaccgca actggtacaa tcagcagtca agcgatcctt
     6661 tcaagacctc aacggcaatg ggtgagaccg gatatctgaa actttctggc aatattgtta
     6721 tgagagtgat gcctgaaaag atggaaggga ttccgcttta tcttaaatca tctgattaca
     6781 acatcctgac aggaaatgtc tggcactcaa gatcaaaaga ttcaatgccc gtttttcctg
     6841 atggtgaaat ggagtggcag ttattcggcg aaggtgaggg aaaaaggaaa gcttctcttt
     6901 cagtatggat gggaaagaac ggtaaagggg ttctttctct tccaataggt tcaaaaagag
     6961 cgttgaaaat ggatgtcgca ggaattgaaa aaaccgggct tggttcaatt cttgttgagg
     7021 aaggacctga tcttcttgaa tttgttatga cttatgatcc tcagttccgc tatgagccga
     7081 tgcctgaaga aaaagatctc atgatccctg actctgaaaa agaggttata gcaaaggtga
     7141 tgagtgataa tttgctaaaa ggggagacaa attttcaaac ccttgagaac attgaaagat
     7201 attttatgaa tttttcatat actctccaac ttgagccgtc atcagggagg tctgttcttg
     7261 atgattttct taaccgtacg aaagcgggac actgtgaata ttttgcaaca gcgacaactc
     7321 tgatgctcag atctgccggt attgcggcaa gatacagcac cggttattca gttgaagaat
     7381 acagcacact tgaaagatcg ttcattgtca gggcaagaga tgctcatgcc tgggttacgg
     7441 catttgtcgg cgggaagtgg ataacagtgg acacaactcc ttcacagtgg cgctctcttg
     7501 acagggaatc aaagtcgttc attgagccgg tcagagatct gctctcgtgg ctgagattgc
     7561 agtatgaaaa tttcagaaga cagaaaaatg tcgaatttaa cagggcactt atagttattg
     7621 cgactgtgct cacccttttc atgatgataa agatttacat cagaagaaaa agggtttcgg
     7681 gaacagagtt gaaaagcgac cgtttcagcg gaaaagttga tggactcgat tcaccgtttt
     7741 atcaggtgct catcaaatgc tcttctgaag gaatcacgaa aaatgacaat gaaacactcc
     7801 gcaaatgggt gataaaaaat tcagaaaaac tcaaagattc caaagacata atgaaaatga
     7861 tacatcttca cgaacaactg cggtttgatc ccgcatcacc gaaaaatgac atcaggaacg
     7921 aacttcagaa atattgcgac aagtggatcg caggggaatc ttgaaacaat tttttctcct
     7981 gattttcatc gactaaataa tttgcatcat atttcttgca aatataatct gattactgca
     8041 ttatctattg gtgactaaat ttttggagtc ggaaaatgg
//
LOCUS       DAOOYB010000127         8021 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_10045_length_8021_cov_10.474140, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000127 DAOOYB010000000
VERSION     DAOOYB010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8021
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10045_length_8021_cov_10.474140"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            156..1811
                     /locus_tag="PL056_08385"
     CDS             156..1811
                     /locus_tag="PL056_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HPM47072.1"
                     /translation="MTIRDEFAKWKEGAYGKTVAKFPERKKEFKTSFNEVIEPVYVPG
                     FEDPQYLETRGLPGLYPFTRGVQSTMYRGKFWTMRQYAGFGTAEESNKRYKYLLGAGQ
                     TGLSVAFDLPTQIGYDSDHPMSDGEVGKVGVAIDSLKDMEILFDGIPLGEVSTSMTIN
                     STAGVLLAMYIAVAEKQGFTPDKLNGTIQNDILKEYIARGTYRFPPKPSMRLITDIFE
                     YCAANVTKWNTISISGYHIREAGSNAVQEVAFTLADGIAYVQAAIKAGLDVDVFAPRL
                     SFFFNAHNNLLEEVAKFRAARRLWAEIMKNRFNAKDVKSQMLRFHTQTAGCTLTAQQP
                     DNNIVRVAIQTLAATLGGTQSLHTNSRDEALALPTEDSVRIALRTQQIVAYESGIADT
                     IDPLAGSYFVEALTDSIYAKAKKYIDRIDELGGMVEAIEKGFVQQEIQDSAYTYQLEV
                     EKGEQIVVGVNKFQVKELPPSNLLKVDRSIQVSQIAKIKDVKASRDNDAVVKKLAEIK
                     AAAQGTENLMPKILEAVKVYATLGEITGTLQEVFGEHKESVVL"
     gene            1821..3305
                     /locus_tag="PL056_08390"
     CDS             1821..3305
                     /locus_tag="PL056_08390"
                     /inference="COORDINATES: protein motif:HMM:NF025224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HPM47073.1"
                     /translation="MRWMPFIVTILGALLIFSCIGCSDEKNKVDSDSISIADKDASVD
                     YDNAVDEEVEDNDFNDETKDLDEDFPDEDSDSVAQYASCYDEIPDEPYGGLFADPNLE
                     QQIKSKLGIGDDEELKESDLEQITDLYVRQIKDFRGLEKLVNLEKFGFSVGGNIYDFT
                     PLSGLKKIKKFTIFYNDLNDFDNMNCLDGSFSKLTSIEELEINGTELKDVSPIEQLTN
                     LKILDISENQIEFLPENMENLQKLEELYLTYNNIEDITPLNKLTNLTVLFFHYNKVTD
                     ISAIENMKKLVRFGCADNKVVDISSVVNLNNLERLTLDQNQINKIPDKIGSLKKLNIF
                     NLAYNKISSLPSLKGLDSLKTLYLGHNEISDMTPLDSLENLEVLSIPWNKITKVPILK
                     NLKSLETLWLALNEITDLSGFSDNDSFPVLKKVDLNYNKIENAEPLRKREGMGSLSIR
                     ENCIKDLSPLEELKESGCSISGMDEQLERCEDTMLTLAGEMKWK"
     gene            3296..>4458
                     /locus_tag="PL056_08395"
     CDS             3296..>4458
                     /locus_tag="PL056_08395"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM47074.1"
                     /translation="MEVAQIIALGENEKIEFKESFSKEVVISLVSFANFHGGTVYVGI
                     TNNGKVKGVSINQETVQQYINEMRNSTQPVIVPEVSIEEVAGGKKILIISISEYPIKP
                     VSYKGRYYIRRKNSNHVMSVDEVINSRLQSMNTSWDCYHDPYHGMKDISFEKVTEFIN
                     RINAIRGKKIEDDPKTILRKFELIREEKLTLGCFLMFMKDPSIVSTIELGRFQTDTII
                     KDALTLRTDLFSEVDQVLEFITKHINKRYVFTGSVMREERWDYPLEALRELVVNAIVH
                     RDYSSPVDTVIKIYDNRIEFFNSGGLQGDISVDEIINGKLSSKPRNKLIAAMFKDAGI
                     IEKYGSGIKRVVEEFRKYGLPDPVFKVSPNDFHVTVFTFPENETVNETVNETV"
     assembly_gap    4459..4558
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4559..4787
                     /locus_tag="PL056_08400"
     CDS             <4559..4787
                     /locus_tag="PL056_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010698143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HPM47075.1"
                     /translation="NETVNETVNETVKLSKIQKEIIKEIKNNEEITYEELAEKLKKGR
                     ATIHRHIQDLIKKGTIKRVGSAKSGYWKINE"
     gene            4934..5329
                     /locus_tag="PL056_08405"
     CDS             4934..5329
                     /locus_tag="PL056_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013389137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HPM47076.1"
                     /translation="MSQKIRVLIAKPGLDGHDRGAKFVARALRDAGMEVIYTGLRQTP
                     EAIVNAAVQEDVDSVGLSILSGAHNTLLPKVVELLKEKGAEDILVFGGGVIPESDIPF
                     LKEQGVKAVFTPGTPSEEFIKFIKENVKK"
     gene            5329..6267
                     /gene="meaB"
                     /locus_tag="PL056_08410"
     CDS             5329..6267
                     /gene="meaB"
                     /locus_tag="PL056_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008859877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HPM47077.1"
                     /translation="MERPLASDFRVLAKLITKIENDRYGSEDILRDLYKENSETIVIG
                     ITGPPGAGKSSLTDRIIKEYRNLGKTVGVVAVDPSSPFTGGAILGDRIRMCDHATDPG
                     VFIRSMGSRGSLGGLAPATIDVLNLMKSAGFDVLIVETVGVGQSEIDVMSIADTVLLV
                     LVPGMGDDVQALKAGIMEIADIFVINKADKDGKERLAAELNMIINLNRHRLEWEPPVV
                     ETVATENKGTVELMEKISDRGMWLKNKGMSAKKDKIINQFKHMALDRIGANILAILNE
                     QGMFTRWSGDLMNGKTNPYQAMKELDELIKIKWGEK"
     gene            6264..6665
                     /gene="mce"
                     /locus_tag="PL056_08415"
     CDS             6264..6665
                     /gene="mce"
                     /locus_tag="PL056_08415"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020677391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HPM47078.1"
                     /translation="MTRKINHIGIAVNNIQDFVTLYRDVLGLKYSGEEIVEEQKVKVA
                     FFDIGDSRIELLEPTSPDSPVAKFIEKRGEGMHHISMSSDNVEKDIEAMKNKGIKMID
                     ETPRNGAHGTKIAFIHPKETKVLIELTEEKH"
     gene            6694..>8021
                     /locus_tag="PL056_08420"
     CDS             6694..>8021
                     /locus_tag="PL056_08420"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HPM47079.1"
                     /translation="MVTIEEKLADLRSRKEKGKLGGGQKRIDAQHEKGKLTARERMSK
                     LLDPGSFEEFDALRCGTTPGSPVGDGVITGYGTIEGRKVFVFAQDFTVAGGSLGKIVG
                     EKICKVMDMAMKVGAPVIAMNDSGGANIPEGVLSLNGYGEIFKRNTLASGVIPQISAV
                     YGPCAGGAVYSPALTDFVFMTETNSYMFLTGPKVVKAVTHEDVTVDQLGGSLVHMTKS
                     GVAHFSHPTEDESIQQIRKLLSFLPSNNMEEPPIVECTDSFDRMEEALNTIVPTNLNK
                     GYDMRDVIHMVFDDGEFYEVMPLYAMNILIGFSRLNGRTVGVVANQPNVLAGCLDINA
                     SDKAARFVRFCDAFNIPLISFVDTPGFLPGTGQEYGGVIRHGAKMLYAYSEATVPKIT
                     IVTRKSTGGAYLAMASKNLRNDLNYAWPTAQIAVMGAKGAIEILMKKELDA"
BASE COUNT         2638 a         1426 c         1772 g         2085 t
ORIGIN      
        1 aaaaaacacg ctacagtata attataagaa catcacccgt caagaaatag tatcaattct
       61 gcgattttgt tttaataatt tttttgcaat ttagatttca ggggttagaa tcggctgaaa
      121 ttttggtttt tcttaattgt ttaatgaggt aaaacatgac tatcagagat gaatttgcca
      181 agtggaaaga aggtgcttac ggcaagacag ttgcaaaatt tcccgaaagg aaaaaagaat
      241 ttaaaacatc attcaacgaa gtgatcgagc cggtttatgt tcccggtttt gaagatccgc
      301 aatatcttga aacaaggggg ctgccgggac tttatccatt tacacggggc gttcagtcaa
      361 cgatgtaccg cggaaaattc tggacaatgc gtcagtatgc agggttcgga acggcggaag
      421 aatcaaacaa aagatacaaa taccttctcg gtgcaggcca gacagggctt tctgtcgctt
      481 tcgaccttcc aacccagatc ggttatgact cagatcatcc gatgagtgac ggcgaagttg
      541 gaaaagtagg cgttgcgatc gattcgctta aagatatgga gattcttttt gacggaattc
      601 cgcttggtga agtttccaca tcaatgacca tcaattcaac tgccggcgtg cttcttgcaa
      661 tgtatatcgc agttgccgaa aagcagggat ttactcctga caagctcaat ggaacaatac
      721 agaacgatat tttgaaagag tatattgcaa gaggaactta ccgttttccg ccgaaaccat
      781 caatgcggct tatcaccgac atttttgaat actgtgcagc aaatgttacc aaatggaata
      841 ctatttcaat ttccggttat catatccgtg aagccggctc aaacgctgtt caggaagtgg
      901 ctttcaccct tgccgacggc attgcctatg ttcaagccgc aataaaagca ggtcttgatg
      961 tcgatgtgtt cgctccgcgg ctttctttct ttttcaacgc tcacaacaat ctgcttgaag
     1021 aagttgcaaa gttcagagcg gcaagacggc tctgggctga gatcatgaaa aaccgcttca
     1081 atgcaaaaga tgttaaatca cagatgctcc gtttccacac tcagaccgcc ggctgcactc
     1141 tcactgctca gcagccggac aacaacatcg tcagagttgc gatccagaca cttgcggcaa
     1201 cacttggcgg aacacagtca cttcatacaa acagcagaga tgaggcactt gcactgccga
     1261 cagaagacag cgtaagaatt gcactccgta ctcagcagat agtggcttac gaaagcggca
     1321 ttgcagacac aattgacccg cttgcaggaa gctatttcgt tgaagcgctc acagacagca
     1381 tctatgcaaa agcaaagaaa tatattgata gaattgatga actcggcgga atggtcgaag
     1441 cgatcgaaaa agggtttgtt cagcaggaaa ttcaggacag cgcttacact tatcagctcg
     1501 aagttgaaaa aggggaacag atcgttgtcg gagtaaataa attccaggtc aaggaacttc
     1561 ctccatcaaa ccttctcaaa gttgaccgtt caattcaggt ttcacagatt gcaaaaatta
     1621 aggatgtgaa agcttcaaga gataacgatg cagttgtgaa aaaactcgct gagatcaagg
     1681 cagcagctca aggaactgaa aacctcatgc ccaaaattct tgaagcggtc aaagtttatg
     1741 caactctcgg cgaaatcaca ggaacattgc aggaagtatt cggcgaacac aaagaatcag
     1801 tagttcttta ggagggcaaa atgagatgga tgccttttat tgttacgata ttgggggcat
     1861 tgctcatttt ttcttgtatc ggttgttcag atgaaaaaaa caaagttgat tctgattcaa
     1921 tctcaattgc tgacaaagat gcttcagttg attatgacaa tgctgttgac gaagaagtcg
     1981 aggataacga ttttaatgac gaaacaaagg atcttgatga agatttcccg gatgaagatt
     2041 ctgattcagt tgcccaatat gctagctgct acgatgaaat ccctgatgaa ccttatgggg
     2101 gattgtttgc tgatcctaat cttgaacaac agataaaaag caagctgggg attggtgacg
     2161 atgaagagct aaaagagtct gaccttgaac agataacaga tttatatgtc aggcaaatta
     2221 aagattttcg tggacttgaa aaacttgtta atcttgagaa attcgggttt tccgttggtg
     2281 gcaacatcta tgattttaca cctttgtcgg gtttgaaaaa aattaagaaa ttcactattt
     2341 tttataatga tttgaacgat tttgataata tgaactgtct tgacggatcg ttttccaagc
     2401 ttacatccat tgaggaactt gaaataaacg gaactgaatt aaaagatgtt tcaccgattg
     2461 aacaactgac aaatctcaag atactggata taagtgaaaa tcaaattgaa tttcttccgg
     2521 aaaacatgga gaatttgcaa aaacttgaag aattatatct tacatataat aacattgagg
     2581 atatcactcc gctcaacaaa ttaacaaatt taacagttct gttttttcat tacaacaaag
     2641 ttacagatat ttctgctatt gaaaacatga aaaaacttgt ccgcttcggc tgtgctgata
     2701 ataaagttgt tgatatatca agtgttgtaa atcttaacaa tttggaaagg ctgactctcg
     2761 atcagaatca gataaataaa attcccgata aaatagggag cctaaaaaaa cttaacattt
     2821 ttaatctggc ttacaataaa ataagtagct tgccatcact gaaagggctt gattcgctta
     2881 aaacgctata tctaggtcat aatgaaatat cagatatgac accgcttgat tcacttgaaa
     2941 atcttgaagt tctttcaata ccatggaata aaattactaa ggtgcctata ttgaaaaatt
     3001 taaaatccct agaaactctt tggttagcac tgaatgaaat tactgatctt tcagggttca
     3061 gtgataatga ttcatttccg gtgcttaaaa aagttgatct caattacaac aaaatagaaa
     3121 acgctgaacc gctcagaaaa agagaaggta tgggatcact ctcaatcaga gaaaactgca
     3181 ttaaagatct atctccactt gaagaattga aagaaagcgg ttgttcaatt tcagggatgg
     3241 acgaacagct tgaaagatgt gaagatacaa tgttaactct tgcaggagaa atgaagtgga
     3301 agtagcacaa atcattgctc ttggtgaaaa tgaaaagatt gagttcaaag aatctttttc
     3361 aaaggaagtg gttatctctc ttgtttcttt tgctaatttt catggtggaa cagtatatgt
     3421 tggaattaca aataatggta aagtgaaagg agtttctatc aatcaggaaa cggtacagca
     3481 atatataaat gaaatgagaa actctactca gcctgttatt gttccggaag tttcaattga
     3541 agaagttgct ggggggaaaa aaatactcat tatttccata tcagaatatc cgattaagcc
     3601 ggtttcttat aaaggcagat actacatccg aagaaaaaac agcaaccatg taatgagtgt
     3661 tgacgaagtt ataaattcaa ggcttcagtc aatgaataca agttgggatt gctatcatga
     3721 cccatatcat ggaatgaaag acatatcttt tgaaaaagtg actgaattta ttaatagaat
     3781 caatgcgata agaggtaaaa aaatagaaga tgatcccaaa accattctga gaaaatttga
     3841 acttatcaga gaagaaaaat tgaccctggg ctgttttctt atgttcatga aagacccgag
     3901 cattgtttca acaattgagc ttggacgatt tcagacagat acaatcataa aagatgcttt
     3961 gacattaaga accgatcttt tttccgaagt tgatcaagtg cttgaattca taacgaaaca
     4021 tataaacaag agatatgttt ttacaggcag tgtaatgcgt gaagaaagat gggattatcc
     4081 acttgaagca ttaagagaac ttgtagtaaa cgcaatagtt cacagagatt attcgtcccc
     4141 agttgacaca gttattaaga tttatgacaa tagaattgag tttttcaatt caggaggatt
     4201 gcagggggat atttctgtcg atgaaataat taatggaaaa ctctcctcta aacctcgcaa
     4261 caagcttatc gcggcaatgt ttaaagatgc cggaataatt gagaaatacg gctcaggaat
     4321 aaaaagagtt gttgaagaat tcaggaaata cggtctgcca gatccggttt ttaaagtttc
     4381 tccgaatgat tttcacgtta ctgttttcac atttccagaa aatgagactg taaatgagac
     4441 tgtaaatgag actgtaaann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnaa
     4561 atgagactgt aaatgagact gtaaatgaga ctgtaaaact tagcaaaatc cagaaggaaa
     4621 tcattaagga aatcaaaaat aatgaagaaa taacttatga agagttagcg gaaaaactga
     4681 aaaagggaag agccacaata cataggcata ttcaggatct tattaaaaaa ggaaccatta
     4741 aaagagttgg atctgccaaa tcaggatatt ggaaaataaa cgagtaaatg atgaagattt
     4801 tatgcacaac gataagttca tcgtatgtaa gcagcgcagc atcttgcaat tcaacttgca
     4861 atggctatga tgaaacgaat tgtgaagaag atggctggta attgataata ttttagttgc
     4921 caaggagggc aaaatgagtc agaaaataag agttttaatt gcaaaaccgg gtctggatgg
     4981 tcacgatagg ggtgcaaaat ttgttgcaag ggcacttaga gatgccggta tggaagtaat
     5041 ttacacaggt ctgcgtcaga ctcctgaagc aattgtcaat gcggctgttc aggaagatgt
     5101 cgattcagtc ggcttgtcta ttctcagcgg tgcgcacaac acacttctgc cgaaagttgt
     5161 tgaactgtta aaggaaaaag gggctgagga cattctcgtt ttcggcggcg gagtaattcc
     5221 tgaaagcgat attccatttt tgaaggaaca aggggttaaa gcggttttca ctcctggaac
     5281 tccgtcagag gaattcatta aattcattaa ggaaaatgtg aaaaaataat ggaaagacct
     5341 cttgcgtccg actttagagt gctggcaaag ctcataacta aaattgaaaa tgaccgatat
     5401 ggatcggaag atattttaag agacctttac aaggaaaatt ctgaaacgat agttatcggg
     5461 atcactgggc ctccgggggc aggcaaaagc tcactcaccg acaggatcat taaggaatac
     5521 agaaacttgg gaaaaaccgt cggagttgtc gcagttgacc cttcatcccc ttttaccggt
     5581 ggagcgatcc tcggtgacag gataagaatg tgcgaccatg cgaccgatcc cggagttttc
     5641 atccgttcga tgggaagcag agggagtctg ggcggacttg cacctgctac aattgatgtt
     5701 ctcaacttaa tgaaaagtgc cggttttgat gtcctgatag ttgaaactgt gggagtagga
     5761 cagagcgaaa tcgatgtcat gagcatagcc gacacagtgt tacttgtcct tgttccggga
     5821 atgggggacg atgttcaggc gctgaaagcc gggatcatgg aaatagccga tatttttgtc
     5881 atcaacaaag cggacaaaga tggaaaagag cgacttgcag cggaacttaa catgataata
     5941 aatctcaacc ggcaccgtct tgaatgggaa ccgccagttg ttgaaaccgt tgcaacggaa
     6001 aacaagggaa cagttgaact gatggaaaaa atatctgaca ggggaatgtg gcttaaaaac
     6061 aagggtatgt cggcaaaaaa agacaaaata atcaatcaat tcaaacacat ggctcttgac
     6121 agaattggag cgaatattct tgcaatttta aatgagcaag gtatgttcac caggtggagt
     6181 ggtgatttga tgaatgggaa aacgaacccg tatcaggcaa tgaaggaact tgatgaactg
     6241 ataaaaataa aatggggaga aaaatgacaa gaaagatcaa tcacatcggt atcgccgtca
     6301 ataacattca ggattttgtg actttgtacc gagatgttct cgggctgaaa tattcgggtg
     6361 aggaaattgt tgaagaacag aaagtcaaag ttgctttctt tgatatcggt gacagtagaa
     6421 ttgaacttct tgagccgaca tcacctgaca gccctgttgc aaaattcatt gaaaaaagag
     6481 gtgaagggat gcaccacatt tccatgagca gcgataatgt tgaaaaagac attgaagcaa
     6541 tgaaaaataa agggataaaa atgatagatg aaactccaag aaacggagca cacggaacaa
     6601 agatagcgtt catccacccc aaggaaacaa aagtgcttat tgagctcacc gaggaaaaac
     6661 attaatattt tcaactgaat ttggagtatg gaaatggtaa ccattgaaga aaaactggca
     6721 gatcttaggt ccagaaagga aaaagggaaa ctgggtggcg gtcagaaaag gatcgatgct
     6781 cagcatgaaa aaggaaaact gacagcccgc gagaggatgt ctaaactcct tgatcccgga
     6841 agttttgaag aatttgatgc tctccgctgc ggcacaaccc ccggaagtcc tgtaggagac
     6901 ggcgttataa ccggatacgg aaccatcgaa ggaagaaaag tgtttgtttt tgctcaggat
     6961 tttactgtag ctggcggatc gcttggaaag atcgtcggtg aaaagatctg taaggtgatg
     7021 gacatggcta tgaaagtggg tgcaccagtc atagcaatga atgattcagg cggagctaac
     7081 attcctgaag gggttctttc acttaacgga tatggtgaga ttttcaaaag gaatactctt
     7141 gcaagtggtg tgattccaca gatatctgca gtttacggac cgtgtgcggg cggcgcagtt
     7201 tattcacccg ctctaaccga ttttgtattt atgacggaaa caaattctta tatgttcctt
     7261 accggtccca aagttgtaaa agccgttact cacgaagatg taactgtcga ccagcttggg
     7321 ggatcacttg tccacatgac aaaatcgggt gtcgcccatt tctcacatcc tactgaagac
     7381 gaaagcattc agcaaataag aaaacttctt tctttcctgc cttccaacaa catggaagag
     7441 ccgccaattg ttgaatgcac agactccttt gacagaatgg aagaagccct gaacaccatt
     7501 gttccgacaa atctgaacaa aggatacgac atgagagatg tcattcacat ggtgtttgat
     7561 gacggagagt tttatgaagt catgccgctt tatgcaatga atatcctcat cggattttca
     7621 cggcttaacg gacggactgt cggtgttgtt gcaaatcagc ccaatgttct tgcgggatgc
     7681 cttgacatca acgcttccga caaggcggca agatttgtaa gattctgcga tgcgtttaac
     7741 attcccctta tttcatttgt agatactccc ggattccttc ccggcacagg tcaggaatac
     7801 ggcggagtta taaggcatgg tgccaaaatg ctttatgcat atagtgaagc tacagttccc
     7861 aagatcacga tcgttacaag aaaatcaact ggcggagcat accttgcaat ggcttccaag
     7921 aacttaagga acgatctcaa ctatgcatgg cccacagctc agatagctgt catgggcgca
     7981 aaaggtgcta tcgaaatact tatgaaaaaa gaacttgatg c
//
LOCUS       DAOOYB010000128         7914 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_10250_length_7914_cov_9.041227, whole genome shotgun sequence.
ACCESSION   DAOOYB010000128 DAOOYB010000000
VERSION     DAOOYB010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7914
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10250_length_7914_cov_9.041227"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(167..1528)
                     /locus_tag="PL056_08425"
     CDS             complement(167..1528)
                     /locus_tag="PL056_08425"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPM47080.1"
                     /translation="MGRLRSKKENILIIDDSPDTLEMLHRSLEHMGYSVFSCESATEA
                     IEVMKSERFDLVITDYHMPFIGGIDVIRHVRENYKNTEVMMITGYASVEGAVEAIKAG
                     AEEYLSKPFTDEELEQAISRAMEKLSIRALKSELPSKNISRQYGLIGESEPMKRVAES
                     IKKAASNDLPVLITGEPGTGKELVARIIHYESASSGHPFIHVNCKAVPETILDSEING
                     CKRAEKDGRSESAPGFLETAGRGTVFFDEISATNLSIQVKLLQALAGEITHVGDNSVI
                     KINSRIMASTSRDLIQLIKKGLFREDLFFKLNVSNIVLPPLRERGKDIIIISNAILSD
                     VAKETGKNTVQKLDEKAAKCLLEYSWPGNIRELEQVIRKVASNTDSDVIEISDLPVHM
                     KFDISKERMINRTLSEVEVEHIKAVLEKVEGNKTKAAELLGIDRKTLRDKLKKPDSAM
                     EKK"
     gene            complement(1530..3239)
                     /locus_tag="PL056_08430"
     CDS             complement(1530..3239)
                     /locus_tag="PL056_08430"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM47081.1"
                     /translation="MKNKTNCLNLFNEFHKISFQLLSLADKGTLISEYLQEISKIILD
                     FSKARQLNFIVNDIRNYYRLSSDADGEWVVTAAIQFKEIPDFITRNSTETTIEHLERA
                     VYFKYLKSSELMTVSGGLFIPDTSSQFELSAGYGTIRNKFNVKVITDFKSILILPIRI
                     GSETIGILSLKWAEKKDLTVEEIGLYQYIFDILGFARSHRQAKFHLGERIKELTTIFQ
                     ITRLGAEKNNSIDDIMEGAVEIIPPGFLVPEFACCRIVYMNRTFKSRNFASSKHTLVE
                     EIFVNGEKNGFVEVGYTKEKSSLDKGPFLKEERPMLKTIARELGLIAERKHFEDEKEK
                     LQQQLLHADRLVTVGQLTAGVAHELNEPLGGILGFAQLIKKYGEINDTVEKDVDKIIK
                     AALHAREIIRKLMLFSRQMPPDKVLFNINERIDDGLYLLENRIKKSSIILKKELQPDL
                     PLIKIDPSQFHQVLVNLVVNAIQAMPEGGTLTIKTENLINNISMTVRDTGIGMTEEQK
                     GKIFIPFYTTKGINEGTGLGLPVVLGIVQSHGGSISVESKPGKGSVFTVKFPVTNETS
                     GGK"
     gene            complement(3307..>5331)
                     /locus_tag="PL056_08435"
     CDS             complement(3307..>5331)
                     /locus_tag="PL056_08435"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HPM47082.1"
                     /translation="RHFILTNLGFGKFVFKDGTGKKVIGEAGNMEEFEKLIRTVPIES
                     ILYHAENNHFSTWLMARGEVQFSTILRALNMSNFGEISMLRPFLIEIFDLIKQLKTRG
                     MVINLEEDHSPGCSCIFRLAGGSLGGKGRGVAFINNLIERVNFGIENIDIKIPLTGII
                     GTDAFDEFMETNGLEEFVLSEKDYKKIRERFTSSRLPAGIEKKLMKFIRTVDGPIAVR
                     SSGLFEDMLMQPFAGIYETYFLPNSAKDIKKRFDQLTTAVKLIYASIYSLKARSYFSA
                     LNYEIEEEKMAVMIQKVVGRKFGSYFYPHISGVAQSRNHYPVSYLLPEDGITVIAAGL
                     GAYVVNGDTSFRFCPKYPKMDLVSNRYQINNSQHYLYAIDLKNEEPDIFSGENFCYAQ
                     LPVDEAKKHGSMKNLLSYYDHNDDRIYPGVSEKGTDIVNFANILKYNALPFNEAINAF
                     LEVGHRSMGTPVEIELAFDIDEEKDKGTFYVLQLKPMIHLTDNVEINIDDIKDEELIL
                     FTDKGMGNGKIDDICDIVFVDPENFEKMSTVVIAKEISKINSLLKKENRRYILIGLGR
                     WGSRDTSIGIPVNFADISQSKIIVEAGFKDFQIDASLGSHFFHNITSMNIGYFTVNYQ
                     SEKSFIAWDWLKSQKPFKKLKYVTHLRFKRPLEVLMDGRSGLNIIRKPGS"
     assembly_gap    5332..5431
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5432..6041)
                     /locus_tag="PL056_08440"
     CDS             complement(<5432..6041)
                     /locus_tag="PL056_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HPM47083.1"
                     /translation="MYFRQLLDKIDQYREKRDVFHDLMQFKVQEILLVATLYDSFIME
                     QEGDLGDVVYGEYHQLNLSSAPRITTTYTHKDALKKLSAGKFDMIIIMVGIDFQELKQ
                     LSLNIKELDLGIPATLLFNNNTVYAAFCEKNDPFDYADQAFVWNGDPAIFLAMIKLVE
                     DRANADNDSTLGMVRIVLLVEDSIRYYSRYLPVLYSEIMRQTS"
     gene            complement(6058..>7914)
                     /locus_tag="PL056_08445"
     CDS             complement(6058..>7914)
                     /locus_tag="PL056_08445"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HPM47084.1"
                     /translation="KGEIKSALKLKSAASSDFKDPSEIKAFIKNILLDRWLEKKKDSI
                     SNFSESIFRESGYIMRVSNGSVGGKGRGIAFTESLLQKPQFHEMIKGINVTIPRTVII
                     GTDEFENFMDQFEFSDLLNSDEDHREIRLKFLEAELSSMLSQRLKKMLLYIKSPLAVR
                     SSGLFEDSLSQPFAGIYETYFLPNNHPDINVRLAQLLDAVKMVYASVFTPLAKSYFDS
                     VNHSLEEEKMAVIIQELVGNSYGDHFYPHISGVAQSYNYYPFSHMKPEDGLGLLAAGL
                     GKSIVDGSQSFRFCPKFPKMNVMTAKDLLQNSQRYIYGLNIKNNQLHLENGDEATLEK
                     IGIDIAEKHGSLNLCASVYDNDSNSIKPGLHAPGPRIIDFRYILHYDIVPLANAVTTI
                     LDVMKSALGTPVEIEFSVDLNKDESGKCSLYILQVKPLIRTMENIEVSTDAVDKKDIL
                     LYTTRGMGNGVIEGIKDLVYVIPEKFDKMKTRLIQKEVAGINNEMKKNGLQYILMGPG
                     RWGSSDPFLGIPVDWAAISQAKVIVEAGLKDFNVDASLGSHFFHNITSMNIGYFTVPA
                     KSSDSYIDFEHLKSMEPHNETEFLRHIRFETPLTVIMDGKRSVSIITKEHWK"
BASE COUNT         2007 a         1676 c         1552 g         2579 t
ORIGIN      
        1 atgccgtatt tttcgatgag tttgttgtag aaatcatact cttcgcagaa cattaccggt
       61 ttgtctgttg tttttgcgta tgagcagaaa tcacggttga cgcagttgtc acacagtcct
      121 ttggtttctt tctttttttc agtgcttttt tcagattttt tctctctcat ttcttctcca
      181 tggcagagtc aggttttttc agtttatctc gcagagtttt tctgtcaatt ccaagcagtt
      241 ccgcggcttt ggtcttgttt ccttcaactt tttcaagaac agcttttata tgctcaactt
      301 caacttcgct taatgttctg tttatcattc tctctttgga aatatcaaat ttcatgtgaa
      361 ccggaaggtc ggatatctca attacatcgc tgtctgtgtt tgacgccact ttgcggataa
      421 cctgttcaag ttctctgatg ttgcctggcc atgaatattc aagaagacac ttcgccgctt
      481 tttcatcaag tttctggaca gtgtttttcc ccgtttcctt tgcaacatct gataaaattg
      541 cgttacttat gatgataata tcttttcctc tttctcttaa cggaggaaga actatgttgg
      601 aaacattcag cttgaaaaag agatcttctc tgaaaagtcc tttttttata agctgtatga
      661 gatctcttga agttgaagcc attattctgg aatttatctt tattacagag ttgtcaccga
      721 catgtgttat ctcaccagcc agtgcctgca gcagtttcac ctgaattgaa aggttagtgg
      781 cactgatctc atcaaaaaaa actgttcctc tgcctgctgt ttcaagaaat cccggtgcgg
      841 attcacttct gccgtctttt tcagcccttt tgcagccgtt tatttcgcta tccagaatag
      901 tttcaggcac agctttgcag ttgacatgaa taaaaggatg tcccgaagag gcactttcgt
      961 agtgaataat tctcgcaaca agttctttgc ctgtccccgg ttctcccgtt atgagcacag
     1021 gaagatcgtt tgatgcagct ttttttattg attccgcaac tcttttcatc ggttcggatt
     1081 caccgattaa tccgtactgt cttgaaatgt tttttgaagg gagttcgctt ttcaaagccc
     1141 tgatgctgag tttttccatt gcccttgaga tcgcctgctc aagctcttca tcagtgaacg
     1201 gttttgaaag gtattcttcc gcacccgctt ttatcgcttc aaccgcacct tcaacagacg
     1261 catagccggt tatcatcatc acttcggtat tcttatagtt ttccctgaca tgtctaatga
     1321 catcgatgcc gcctatgaac ggcatgtgat aatctgttat tacaaggtca aatcgttcac
     1381 ttttcattac ttcaatggct tcggtcgcac tttcacagga aaaaaccgaa tatcccatgt
     1441 gttcaagcga ccggtgaagc atttcaaggg tgtccggaga atcatcgatg ataaggatgt
     1501 tttctttttt agatctgagc cgtcccatat tattttcctc ccgaggtttc atttgtaaca
     1561 gggaatttca ctgtaaatac tgaccctttt cccggtttac tttccacact gatagatccg
     1621 ccatgggact gaactattcc aagaaccacc ggaagaccga gacctgttcc ttcgtttatc
     1681 ccttttgtgg tatagaacgg gatgaatatc tttcccttct gttcttctgt cattccgatg
     1741 cctgtgtccc tgaccgtcat ggatatgtta ttgataagat tttcggtctt tattgtcaga
     1801 gttcctccct ccggcattgc ctgaatggca tttacaacga gatttacaag aacctggtga
     1861 aactgtgacg ggtcaatttt tatcagcggc agatcgggct gaagttcttt tttgagaatg
     1921 atgctgctct tttttatcct gttctcaagg agataaagac cgtcatctat cctttcgttt
     1981 atattgaaaa gaaccttgtc aggaggcatc tgtctggaaa aaagcatgag tttgcggatg
     2041 atctctcttg cgtgtagagc cgctttaatt atcttgtcga catctttttc aactgtgtcg
     2101 tttatttcac catatttttt tatcagctgg gcaaaaccga gtatccctcc gagcggctcg
     2161 ttaagttcat gggcgacacc tgcagtaagt tgtcctactg ttacaagacg gtctgcgtga
     2221 agaagctgct gctgaagctt ttccttttca tcctcaaaat gtttcctttc ggctataagc
     2281 cccagttctc ttgcgatcgt tttaagcatc ggtcgctctt ccttgagaaa aggacctttg
     2341 tcaagactgc ttttttcttt tgtgtatccc acctcgacaa acccgttctt ctctccgttg
     2401 acaaatatct cttcaacaag ggtgtgtttc gatgacgcaa agtttctgct tttaaatgtc
     2461 ctgttcatat atactatcct gcagcatgcg aattcaggaa ctaaaaaacc gggaggaatt
     2521 atttcaacag caccttccat aatatcatcg atagagttgt tcttttcagc acccagacgg
     2581 gttatctgaa atatggtcgt aagctccttg atcctttcac cgagatgaaa ttttgcctgc
     2641 cggtgcgatc ttgcaaaacc gagaatgtca aaaatatact gatagagccc tatctcttcg
     2701 acagtcagat cttttttttc tgcccattta agtgacagta tcccgatggt ctctgatccg
     2761 atcctgatcg gaaggatgag gattgacttg aagtcggtga taactttgac attaaattta
     2821 tttcttattg ttccgtaacc ggctgaaagt tcgaactgag atgatgtgtc aggtatgaaa
     2881 agaccccccg atacagtcat tagctctgac gatttcagat atttaaaata gacagccctt
     2941 tcaagatgtt caattgttgt ttcagttgaa ttccttgtta tgaaatcggg gatctcttta
     3001 aactgtattg cggctgttac aacccattcc ccgtcagcat ccgaactcaa tctgtaatag
     3061 tttctgatat cgttcacaat aaaattaagc tgccgggctt ttgaaaaatc gagaatgatc
     3121 ttcgagatct cctgaagata ttcgcttatg agcgttcctt tgtcggcaag actgagaagc
     3181 tggaatgaga tcttgtgaaa ttcattaaat aaattcaagc agttcgtttt gtttttcatt
     3241 gtcttcccaa aagaagtcca tcaaggccgg ttttatcaca aataatgatc aaaatcaatc
     3301 tccgtttcaa gatccaggtt ttctaatgat attaagaccg cttctcccgt ccataagaac
     3361 ctcaagcggt cttttgaatc gcagatgtgt gacatatttg agctttttaa aaggtttttg
     3421 agatttaagc cagtcccatg cgataaatga cttttccgac tgataattga cagtaaagta
     3481 cccgatgttc atggatgtta tgttgtgaaa gaaatggctc ccaagagatg catctatctg
     3541 aaaatcctta aaacctgctt caactattat ttttgactgc gatatgtctg caaaattgac
     3601 aggaatgcct attgaagtgt ctcttgatcc ccaccggccc agtccgataa ggatataccg
     3661 cctgttctcc tttttaagta aagaattgat ctttgatatt tcctttgcga tcacaacagt
     3721 tgacattttt tcaaaatttt cagggtcaac gaaaactatg tcgcagatat catcaatttt
     3781 tccgttgccc atccctttgt cagtaaaaag tatcaactct tcatctttga tgtcatctat
     3841 atttatttcc acattatcgg tcagatgaat catcggtttc agctgtaaaa catagaatgt
     3901 ccccttgtct ttttcttcgt cgatatcaaa tgcaagctct atctctaccg gagttcccat
     3961 cgatctgtgt ccgacttcaa gaaatgcgtt gatggcttca ttaaagggga gtgcgttgta
     4021 tttcagtata tttgcaaagt tcacaatatc agtccctttt tcagatactc ccggatatat
     4081 tcggtcatca ttgtggtcat agtagcttaa gaggtttttc atcgaaccgt gtttcttcgc
     4141 ttcatcgaca gggagctgtg cgtagcagaa gttttccccg ctgaatatat cgggttcttc
     4201 atttttaaga tcgatcgcat atagatagtg ctgagaatta tttatctgat aacggttact
     4261 gaccagatcc attttcggat acttcgggca gaacctgaaa gaagtgtctc cgttcactac
     4321 ataggcgccg agtccagcag ctatgacagt gatgccgtct tcgggcagaa gataagaaac
     4381 cggatagtga tttcttgact gtgcaactcc gcttatgtgg ggataaaaat aactgccgaa
     4441 ttttctgccg actaccttct ggatcataac ggccattttc tcttcttcaa tttcatagtt
     4501 gagcgcactg aaatatgatc tcgctttaag cgaataaatt gaagcatata tcagctttac
     4561 agcagttgtg agctgatcga accgcttttt tatatctttt gccgaattag ggagaaaata
     4621 tgtttcatat atccctgcaa acggctgcat cagcatatct tcaaaaaggc ccgaacttct
     4681 gacagcaata ggtccgtcaa ctgtcctgat gaacttcatg agctttttct caattcctgc
     4741 cggcagtctg gacgatgtaa accgttccct gatcttttta taatcttttt cagaaagaac
     4801 gaactcctcc agcccgtttg tttccatgaa ttcatcaaag gcatctgttc cgattatacc
     4861 ggtaagcggt atttttatat caatgttctc aattccgaag ttgactcttt cgataagatt
     4921 gttaataaac gcaacgcctc ttccttttcc acctaaagac ccgcctgcaa gacggaagat
     4981 acagctgcat cccggagagt ggtcttcttc aagattaatg accattcccc ttgttttgag
     5041 ctgtttaata aggtcaaata tctcaatgag aaagggtcga agcatggata tctcaccgaa
     5101 attggacata ttcagagctc tcaatatcgt tgaaaactgc acttcacctc ttgccataag
     5161 ccatgtggaa aaatgattgt tttcagcgtg atagaggatg ctttcaatgg gaacagtcct
     5221 gataagtttc tcaaactcct ccatgttccc ggcttcaccg ataacttttt ttccggtacc
     5281 gtccttgaag acgaatttgc cgaaaccgag attggttaga atgaaatgtc gnnnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nggcttgtct gacgcattat ttcactgtaa
     5461 agaacaggca gatatctgct gtaatatctt atgctgtctt ctacaagaag aactattctg
     5521 accattccca gcgttgaatc attgtctgca ttggcacggt cttcaacgag ttttatcatt
     5581 gcaagaaaaa tggcgggatc accgttccag acaaatgcct gatcggcata gtcaaacggg
     5641 tcgttctttt cacagaaagc ggcataaacg gtattgttgt tgaatagaag ggtagcggga
     5701 atgcccagat caagctcttt aatgttcagg gaaagctgtt ttaattcctg aaaatcgatg
     5761 ccgaccataa taatgatcat atcgaatttt ccggcagaaa gttttttcaa tgcatcttta
     5821 tgtgtgtatg tagttgtgat acggggagcg gaacttaaat tgagctgatg atattcaccg
     5881 tacaccacat caccgagatc cccttcctgt tccatgatga aagagtcata tagagttgcg
     5941 acaaggagga tctcctgaac cttgaactgc ataagatcgt ggaacacatc ccttttttcc
     6001 ctgtactgat ctatcttgtc taaaagctgt ctgaaataca ttttttttcc cgctgattta
     6061 tttccaatgt tctttagtta ttatcgaaac tgatctcttt ccgtccatta tgacggtgag
     6121 aggtgtttca aaccgtatgt gtcttaagaa ttctgtttcg ttgtggggtt ccatcgattt
     6181 gaggtgttca aaatcaatgt atgaatcact gctttttgcc ggaacagtga aatatccgat
     6241 gttcattgag gtaatgttat gaaagaaatg acttcccaaa gaagcatcaa cattgaaatc
     6301 cttcaatccg gcttcaacaa tgactttcgc ctggcttatc gccgcccagt caacaggaat
     6361 gcccaggaac ggatcgcttg aaccccatct gccaggcccc atgagaatat actgcagtcc
     6421 gttctttttc atttcattgt ttataccggc gacttctttc tgaatgagac gggttttcat
     6481 tttgtcaaac ttttcaggaa tgacatacac aaggtccttt atcccttcga tcactccgtt
     6541 tcccattccc cttgttgtgt atagaaggat atctttttta tcgacggcgt cagttgacac
     6601 ttcaatgttc tccatagttc tgataagcgg ttttacctga agtatgtaga gagagcattt
     6661 tcctgattcg tctttattga ggtcaacgga aaactctatt tcaaccggag ttcccagtgc
     6721 acttttcatg acatcaagta ttgttgttac ggcatttgca agagggacaa tgtcatagtg
     6781 aagaatatag cggaagtcga ttatcctggg accgggagca tgaagtccgg gttttatgga
     6841 gtttgaatcg ttgtcataaa cggaagcaca taaatttaaa gatccgtgtt tttcggcaat
     6901 gtctatcccg attttttcaa gagtggcttc atcaccgttc tcaagatgca gctggttgtt
     6961 cttaatgttc aatccgtata tgtatctctg tgagttctga aggagatctt ttgctgtcat
     7021 gacattcatt ttgggaaatt tcggacagaa tctgaatgac tggcttccgt caacgatgct
     7081 tttgccaagc cctgctgcaa gaagtccgag cccgtcctct ggtttcatgt gggagaacgg
     7141 gtaatagttg tatgactggg cgacaccgct gatatgagga taaaaatgat cgccgtatga
     7201 gtttccgaca agttcctgga ttattacagc cattttctct tcttccagcg agtggttgac
     7261 agaatcaaaa taactttttg ccagaggggt gaaaacagag gcatagacca ttttgaccgc
     7321 atcaagcagt tgtgcaagcc gtacattgat gtcagggtga ttgtttggaa gaaaatatgt
     7381 ttcgtaaatc cctgcgaaag gttgtgacag actgtcttca aaaagacctg aacttcttac
     7441 tgcaagaggg gatttaatat aaagaagcat ctttttcagc cgttgtgaaa gcatggatga
     7501 aagctctgcc tcaagaaatt tcagacggat ctctctgtga tcttcatctg agttaagaag
     7561 atcactgaat tcaaactggt ccatgaaatt ttcaaattca tcagttccga tgatcacagt
     7621 tctgggaatg gttacattta ttccctttat catttcatgg aactgcggtt tctgaagaag
     7681 tgactcggta aatgctatgc ctcgtccctt tcctccgaca gaaccgttgg aaaccctcat
     7741 gatatatccc gattctctga aaattgattc ggagaaattg gaaatggagt cttttttctt
     7801 ttccagccac ctgtcaagca gaatattttt aataaatgcc tttatttctg aaggatcttt
     7861 gaaatctgat gaagcggcgc ttttaagttt caacgcactt tttatttcac cttt
//
LOCUS       DAOOYB010000129         7913 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_10252_length_7913_cov_15.237083, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000129 DAOOYB010000000
VERSION     DAOOYB010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7913
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10252_length_7913_cov_15.237083"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3641)
                     /locus_tag="PL056_08450"
     CDS             complement(<1..>3641)
                     /locus_tag="PL056_08450"
                     /inference="COORDINATES: protein motif:HMM:NF012433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HPM47085.1"
                     /translation="EFKALPDRSVPEKGKPVFSYRILADVTDISGETRSTSSTVSVGY
                     LALSVSENIPNEINTLKELEFEIATANLNGEFEPAKGIVKISKLQEPDRLLRKRLWNK
                     PDKFLMSKEEYVKDFKSDVYDSEDDITQRKIEKQVFTAQFDTSKEKKIIIKTADQWLD
                     GKYLVEIETKDKYGEKIEYKKYFSAYKPENGVMTEKIFNRFSLVNQTVEPGNSGDFVI
                     GSGAENVSVIFEIQRKNKKSETTFINLNKEKKVISIPVTESDRGNIGYSIMFQKNGRA
                     YHQSGVIQVPWTDKQLKIETLSFREKLYPGQKEEWKIKISGSKGEAVAAEMVATLYDK
                     SLDSFRANGWYLAHHPHNYFQNQWIENISHRTAGSVTYQLDWNSYYGGNYLYYDTLNW
                     FGNYFYNYYGYRYSRNMPMPASAARSRSVDSFDGDETIALGSAAVAEEREESDKSAPM
                     KKDKMGMRGAGGLIDTRAGEQSKNLDQGGSPDKANAPETQVQIRKNMNETAFFFPMLN
                     TNDKGEVIISFTIPEALTKWKFMGLAHTKDLKTGIISKETVTQKDIMVMPNMPRFLRE
                     GDSVIISAKITSMIDKVISGKAELRLFDAITMKPVDKLLNNDAPVQDFAIKPKENTVV
                     NWPVKVPLGVSAVTWRIIAKSGNQGDGEEQTLPVLSNRALVTESLPLPLKPNEKREFL
                     FKKLMDSKSSDTLVNHKLTLEFTSNPVWYAVQALPYLMEYPYECAEQIFSRFYANSLA
                     SHIANSNPNIKKVFDQWKNTDALLSNLQKNEELKSVLLAETPWVLDAQNEEQNKKRVA
                     LLFDLNRMGNELDSALKKLRKLQGYNGGWPWFQGLPESRYITQHIVSGLGKLRNLKVK
                     EADPSMDMAKKGVLFVDNEMRKSYDWLKKNHVDMSKKLIGSMEIHYLYTRSFFTDVPV
                     QKQNLEAYNYWISQAEKYWLGETPYMSAMIAAALWRLDRKDAPKKIIASLRERAIYHD
                     ELGMYFKENMGGYYWYQFPIETQAVIIEAFQEVTKDIKTVDDLKTWLIKMKQVQSWKT
                     TKATVDAIYALLIGGSDWLKNNKNAEITLGNIKVDPAKMGAKTEAGTGYFKVAWNRSD
                     VKPEMGKVTVINPNSNPAWGAVYWQYFEQLDKITPAETPLKLEKKIFKEIQTDKGPKL
                     EPVSNNSLLSPGDRLKVRIVLRTDRDMEFIHMKDMRAAGTEPENVLSRHKYQDGLWYY
                     ESTKDVSTD"
     assembly_gap    3642..3741
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3742..5954)
                     /locus_tag="PL056_08455"
     CDS             complement(<3742..5954)
                     /locus_tag="PL056_08455"
                     /inference="COORDINATES: protein motif:HMM:NF013957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HPM47086.1"
                     /translation="MRKLIVFASAVLFLMLFCCGKPEGDKTEERKEKQAAKDLYIPPE
                     KFSSYESEWKEVAGFESEGKPRSALETVEKIQTLAISENNAPQLIKVLLFKTKFVMNI
                     GEKELPAVIKELEDETVKAKFPANAILKSMLADIYWNYFNSNRYKFYNRTETAGNETG
                     DIATWDLKTIFKRITTFYNESLNESENLKNINLSLFDEIIVKGTKPGVLRPSLYDFLA
                     HRTVDFYSNSETQLTEPANVFEVDSDKYFSPAEEFIKIDIKSDSYDSPSFYAFNIFKD
                     LTKHHLKNGNAEALIDVELKRLDFIKEKSVLENSEELYYKALESLEKKYSSNSMTSMI
                     LYKKAIWHYNRNDPENKWNLKTSKELCEKVIKNYKNSEAVDLCLAHISSITTKNIYFN
                     VEAVNTPQIPFRILVNYTNVKKLFFKVVKVSREEKNNISEYVVEQSKAAKIIADKKPL
                     QEWTVELPDDGDLKNHSTEIKIPALEKGIYAIAAGTDKKYSFAKEALFYAFAEISNLS
                     VISTNEENRTKFLVADRESGQPVKGAEIKVFEKEYDYSVSRYVTKHIDTLKTDDSGLA
                     TYKKGWFKNSYGHKFILASKDGNELSTEFYQPYPREPKAYEKTVFFTDRAIYRPGQTV
                     YFKGIMLKFDKEQKPEILKNKKTVVEFYDVNRQKIASLNLKSNDFGSFQGSFTAPNNV
                     LGGNMTIRNKNGAVSFSVEEYKRPKFETNIEPVKESFKINDLVKVKGKAFSYSGAP"
     gene            complement(6035..7696)
                     /locus_tag="PL056_08460"
     CDS             complement(6035..7696)
                     /locus_tag="PL056_08460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HPM47087.1"
                     /translation="MDWKLVIFQTVGGLGLFLMGMKIMSEGMQKAAGDRLRKILKVLT
                     TNRFVGVFVGFLITAIIQSSSATSVMAIGFVNATLMTVQQAIGVELGAAVGTTVTGWI
                     VTMDISAYAMPIIGIGVFIRFFSKSKTWQYMGEIMFGFGILFLGMSSMKDGFAPLKNS
                     EQFISLFKSIDGHTYSSVILGVFVGTITTAVVQSSSAVVGIVIALASQGLINFDGSLA
                     IVMGSNIGTTITGILASIGGSVNAKRAALAQTLFKTAGVILMLTVFYPFKNLVEMITP
                     GLSSINLTVHIAMGHTLFNVINLVVFLPLVGPLAKLVNSIFPDKAKKDDDLPENFISI
                     DYNMIETPSMAIMESEKELLVMSEYVINSMNLLRQMPVKEQDKAVEICDIILKNEDRI
                     DKYQFYITQFLLSLSSRALTLKDANIVGSYIGLAHNLEKVADYVENISAIIDKMRRKQ
                     IDFSNNAVEIINRILDENISYFSESMEFFKSDVKTAAYTEKALTKSIRLKKMVKDAKI
                     EHFDRIREKVCQGGAAIHFIDILNNLDAMSSENYNIAQVVSGKKY"
BASE COUNT         2010 a         1616 c         1479 g         2708 t
ORIGIN      
        1 aagtcggttg agacatcttt ggttgattcg taataccaga gtccgtcctg atatttgtgt
       61 ctggaaagga cattttcggg ttctgttcct gcggctctca tatctttcat gtggatgaat
      121 tccatgtcac ggtcagttct taaaacgatc ctcactttga gacgatctcc ggggctaagg
      181 agcgaattgt tgctcacggg ttcaagtttc ggacctttat ctgtctgaat ttccttgaat
      241 attttctttt caagcttaag tggtgtttca gccggagtaa ttttgtcaag ctgttcaaaa
      301 tactgccagt aaaccgctcc ccatgccgga tttgaattgg ggtttataac agtcactttt
      361 cccatttcag gtttcacatc gcttctgttc cacgcaactt tgaaataacc ggtccctgct
      421 tcggtttttg ctcccatttt agctggatca actttgatat ttccaagagt tatctcagca
      481 tttttattgt ttttgagcca gtcgcttccc cctatcaaca gggcataaat tgcatcaaca
      541 gtcgcttttg tcgttttcca actttgaacc tgtttcattt ttataagcca tgtcttgagg
      601 tcgtcaactg ttttgatgtc ttttgtaact tcctgaaatg cttcaataat tactgcctga
      661 gtttcgatcg ggaactgata ccagtaatat ccgcccatgt tttccttgaa atacattcca
      721 agctcatcgt gataaatagc tctctctctt aatgatgcaa tgatcttttt tggagcatct
      781 tttctgtcaa gccgccagag agccgccgct atcattgcag acatatatgg agtttcgcca
      841 agccagtatt tttctgcctg ggatatccag taattatatg cctcaagatt ctgtttctga
      901 accggcacat cagtaaaaaa gcttcttgtg taaagatagt gtatctccat ggaaccaatg
      961 agttttttgc tcatatccac atggtttttc ttcagccagt catagctctt tctcatttca
     1021 ttatcaacga acagcacccc tttttttgcc atgtccattg acggatctgc ttctttgact
     1081 ttaaggtttc taagctttcc aagacctgaa acgatgtgct gagtgatata tctgctttca
     1141 ggtaacccct gaaaccatgg ccagccgccg ttgtaaccct gtaattttct tagttttttc
     1201 agcgcagagt caagttcatt ccccatcctg ttcagatcga ataacagtgc aactcttttt
     1261 ttattctgtt cttcgttctg tgcatccaga acccacggag tttcagcaag cagaacgctt
     1321 ttaagctctt catttttctg aagatttgaa agcaatgcat ctgtgttttt ccactgatcg
     1381 aacacttttt taatgttggg atttgaattt gcaatgtgac ttgcaagaga atttgcataa
     1441 aatcttgaaa atatctgctc agcacattcg taaggatact ccatcagata cggcaacgcc
     1501 tgaaccgcat accagacagg attacttgta aattcaagtg taagcttgtg atttacaagt
     1561 gtgtcagaac tctttgaatc cattagcttt ttaaacaaga actctctttt ttcattcggc
     1621 tttaaaggta atggcagaga ttccgtcaca agggctctgt ttgataaaac cggcagagtt
     1681 tgctcttctc catcgccctg attcccagat tttgcaatga tccgccaggt aacggcacta
     1741 actccgagag gaactttaac cggccaatta actactgtat tttctttggg ttttatcgca
     1801 aaatcctgaa cgggagcatc gttatttagg agtttatcaa ccggtttcat ggttattgcg
     1861 tcaaaaagac ggagctcagc ttttccgctt atcactttat cgatcatact cgttatcttt
     1921 gcactgataa ttacagaatc accctctctt aaaaatctcg gcatattggg catcaccatg
     1981 atgtctttct gagtgacagt ttcctttgaa attataccgg ttttcagatc ttttgtgtgg
     2041 gcaagcccca tgaatttcca ttttgtaagt gcttcaggaa tggtgaaaga aatgatcact
     2101 tcccctttat cgtttgtatt gagcatcggg aagaagaatg cggtttcatt catgtttttg
     2161 cggatttgca cctgtgtttc aggagcattt gctttatcgg gagatccgcc ttgatcaagg
     2221 tttttgcttt gttcaccagc tcttgtatca atcaatccgc cagctccccg catgcccatt
     2281 ttatcttttt tcattggtgc cgatttatct gactcttctc tttcttcagc cacagccgca
     2341 gagccaagag caatagtttc gtcgccatca aacgagtcaa cagaacggga tcttgcagct
     2401 gaagccggca tcggcatgtt tctgctgtaa cggtagccgt aataattata aaaataattg
     2461 ccaaaccagt taagtgtgtc ataatacaag taatttccgc cataatagct gttccagtca
     2521 agctgatatg tgacggaccc ggcagttcta tgggaaatgt tctcaatcca ttgattctga
     2581 aaataattat gaggatgatg tgcaagatac cagccgtttg ccctgaaact gtcaagagat
     2641 ttgtcataaa gcgttgcaac catttctgct gctaccgcct ctccctttga tcccgaaatc
     2701 ttgattttcc actcttcttt ctgccccggg taaagttttt ctctgaatga aagagtttct
     2761 attttcagct gcttatctgt ccagggaacc tggattacgc ctgactggtg atacgctctg
     2821 ccgtttttct ggaacattat cgaatacccg atgttacctc tgtctgattc ggtcacaggg
     2881 atggagatga ctttcttttc cttgtttaaa ttgatgaaag ttgtttcgct ttttttgttt
     2941 ttgcgctgaa tttcaaaaat tacagaaaca ttttctgcac cgctccctat cacgaaatct
     3001 ccgctgtttc ctggctcaac ggtttggttt acaagggaaa atcgattaaa tattttttca
     3061 gtcattactc cattctcagg tttatacgct gaaaaatatt ttttatattc tattttttca
     3121 ccgtatttat cttttgtttc gatctcgaca agatatttgc cgtcaagcca ctgatctgct
     3181 gtttttataa ttattttttt ctcttttgaa gtgtcaaatt gtgccgtaaa cacctgtttt
     3241 tcgatctttc tttgagttat atcatcttca gaatcataaa catcagattt aaaatcttta
     3301 acatattcct cttttgacat caggaactta tccggcttgt tccaaagccg ttttcttaat
     3361 aatctatccg gttcctgcag ttttgagatc ttgacaattc cctttgccgg ctcgaattca
     3421 ccgtttaggt ttgccgtggc tatttcaaat tcaagttctt ttaaggtgtt tatttcattg
     3481 ggaatgtttt cgctgactga caacgcgagg tatccaacgc ttactgttga ggaagtgctt
     3541 cttgtttctc cgctgatatc tgtgacatcg gctaagatcc ggtaactgaa aaccggcttt
     3601 cccttttcag gaacagatct gtcgggaagt gctttgaact cnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nggagcaccg gaatatgaaa atgccttccc tttcactttt
     3781 acgaggtcat ttattttaaa actttcttta accggttcga tatttgtttc aaacttaggt
     3841 cttttatatt cttctacgct gaaagatacg gctccgtttt tgtttctaat cgtcatgttt
     3901 ccgccaagaa cattattcgg agcggtgaaa gacccctgaa aagagccgaa atcattggat
     3961 tttaagttca agcttgcgat tttctgccgg ttcacatcat aaaattcaac cacggttttt
     4021 ttgtttttaa gaatttccgg tttctgctct ttatcaaatt taagcattat ccctttgaaa
     4081 taaactgtct gaccaggcct gtaaattgcc ctgtcggtga aaaacacagt cttttcataa
     4141 gcttttggct ctcttggata cggttgataa aactctgttg aaagctcgtt cccgtctttg
     4201 gaagcaagaa tgaatttatg tccgtaagaa tttttaaacc accctttttt atatgtcgcc
     4261 agaccggaat cgtctgtttt aagggtatca atgtgcttag tgacataacg gctgacactg
     4321 taatcatatt ctttctcaaa aacctttatt tccgctcctt ttaccggctg acccgattct
     4381 ctgtctgcaa caagaaattt tgttctgttc tcttcatttg tgctgatgac agacagattt
     4441 gaaatctcgg caaatgcata aaacagagct tcttttgcaa atgaatactt tttgtccgtt
     4501 cctgcagcta ttgcataaat tcctttttca agagccggaa tttttatttc tgttgagtgg
     4561 tttttcagat ctccgtcatc tggaagttca accgtccatt cttgaagagg ttttttatca
     4621 gcaatgatct ttgccgcttt tgactgctct acgacatatt ctgaaatgtt gtttttttct
     4681 tctcttgaaa ctttaactac tttaaaaaaa agctttttta catttgtgta attaacaaga
     4741 attctgaacg gaatttgggg agtattgact gcttcaacat taaaatagat gttttttgtt
     4801 gttatggaag agatgtgtgc aaggcagaga tctacggctt cagagttctt gtaattttta
     4861 atgacttttt cacaaagttc ttttgatgtt ttgaggttcc atttattttc gggatcattt
     4921 ctgttgtaat gccagatggc ttttttgtat aaaatcattg aggtcatact gtttgaggaa
     4981 tatttctttt caagagattc aagcgctttg taatatagtt cctcgctgtt ttcaagaact
     5041 gatttttctt taataaaatc aagccgtttc agttcaacat ctataagtgc ttcagcattt
     5101 ccgtttttca gatggtgctt tgtaaggtct ttaaaaatat tgaatgcata aaacgatggt
     5161 gaatcgtaac tgtctgattt tatgtcaatt tttataaatt cctctgcagg tgaaaaatat
     5221 ttatcactat ccacttcaaa aacatttgcc ggctcagtca gttgggtttc gctgtttgaa
     5281 tagaagtcaa ccgtgcggtg ggcaagaaag tcataaaggg acggtcttaa aacgcccggc
     5341 tttgtccctt tcacgattat ttcgtcaaaa agggacaggt tgatattttt aagattttcg
     5401 ctttcattga gcgattcgtt gtaaaaagtt gtaattcttt tgaatattgt tttcaagtcc
     5461 catgtggcaa tgtctcctgt ttcatttccc gccgtttctg ttctgttgta gaatttgtag
     5521 cggttactgt tgaaatagtt ccagtaaata tctgcaagca ttgatttcag aatcgcattt
     5581 gccgggaatt ttgctttcac ggtttcatct tcaagttctt taataactgc cggcaactcc
     5641 ttttctccga tattcataac gaactttgtt ttgaacagaa gcacttttat taattgagga
     5701 gcgttgtttt cgctgattgc aagtgtttga attttctcaa ctgtttcaag agcagacctc
     5761 ggttttccct cagattcaaa gccggcgacc tctttccatt cactttcata gctgctgaat
     5821 ttttcaggcg ggatataaag atcttttgca gcttgtttct cttttctttc ctcagtttta
     5881 tcaccttccg gctttccgca gcagaaaagc atcagaaaca aaacggctga agcaaaaacg
     5941 atcagttttc tcataatttt ctccaaagat ggttgcgaaa cagaaaatta tagtgaaaaa
     6001 atgaatgttt atcaagtttt gaggaatgac aaggtcagta ttttttgcct gaaacgacct
     6061 gggcgatgtt atagttttca gaactcattg catcaagatt gttaagaata tcaatgaaat
     6121 gaatagccgc gcctccttga cagacctttt ctcttattct gtcaaaatgt tcgatcttgg
     6181 catccttgac cattttttta agccgaatgc tctttgtaag ggctttttca gtatatgccg
     6241 ctgtttttac atcactttta aagaattcca tcgattcaga aaaataactg atattctcgt
     6301 caaggatcct gttgataatt tctacggcat tgtttgaaaa atcaatctgc tttcttctca
     6361 ttttatcaat gatcgcagaa atgttttcca cataatctgc aactttttca agattgtggg
     6421 caagcccgat gtagcttccg acaatgtttg catcttttaa agtgagagct cttgaagaca
     6481 gggaaagaag gaactgggtt atatagaact gatatttatc tatccggtct tcgttcttta
     6541 aaataatgtc acagatctca acggctttgt cctgctcttt tacaggcatc tgtcttaata
     6601 aattcatgct gttgataaca tattcactca tcacaaggag ttccttttca gattccatta
     6661 tagccattga aggggtttct atcatgttgt agtcaatgct gataaagttt tcaggcaggt
     6721 catcgtcctt ttttgctttg tctggaaaaa ttgagttgac aagttttgcc agcggtccga
     6781 cgagaggaag aaaaacaaca aggttgataa cattgaaaag agtgtgtccc attgcaatat
     6841 gcacagtgag atttattgaa gaaagtccgg gcgttatcat ttcaacaagg tttttgaaag
     6901 gatagaatac ggtgagcatc agaattacgc ccgcagtttt aaaaagggtc tgggcaagtg
     6961 ccgccctttt tgcatttacc gatccgccga tgcttgcaag aattccggtg atggttgttc
     7021 cgatattgct tcccatgaca attgcaagcg agccgtcaaa attaatgagt ccctgagatg
     7081 caagggcgat aactataccg acaacggcag agctcgactg gacgacggca gtagttatcg
     7141 ttcccacaaa aacacctaat attactgaag aatatgtgtg tccgtcaata cttttaaaaa
     7201 gtgaaatgaa ctgttcgctg ttcttgagcg gggcaaatcc gtctttcatt gaactcatgc
     7261 cgagaaacag tatgccgaac ccgaacatga tttcgcccat gtactgccat gttttgcttt
     7321 ttgaaaaaaa tcttatgaaa acgccgattc ctattatcgg cattgcataa gcggaaatgt
     7381 ccattgtgac tatccagccg gtgaccgttg tgcccactgc ggcaccgagt tcaactccga
     7441 ttgcctgctg aacggtcatg agcgtggcat tcacaaaacc gatcgccatt actgaagttg
     7501 ccgaacttga ctgaatgata gcggttatca gaaatccgac aaaaacaccg acaaaacggt
     7561 ttgttgtgag tactttcagg attttccgca gacggtctcc tgccgctttc tgcatccctt
     7621 cgctcatgat cttcatcccc atcaggaaaa gaccgagccc gccaactgtt tgaaatatga
     7681 ccagtttcca atccatgaat atctccggaa ccgtaattgt taagaaacag ttaattttcc
     7741 gttaaggata ttagcaggaa aagatttcaa ttcaactaaa tttgtaaaaa agttcttttg
     7801 tgaaatatta cttcaaaaga aaatcaagaa tgtgtttaag tgcctgatca taatagactt
     7861 tttcttcatt caggatttca tgaagtgcgt tttcgatgac tatttttctg ctg
//
LOCUS       DAOOYB010000130         7909 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_10261_length_7909_cov_11.980392, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000130 DAOOYB010000000
VERSION     DAOOYB010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7909
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10261_length_7909_cov_11.980392"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            291..707
                     /locus_tag="PL056_08465"
     CDS             291..707
                     /locus_tag="PL056_08465"
                     /inference="COORDINATES: protein motif:HMM:NF024845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HPM47088.1"
                     /translation="MLRCPVCAKVDMAVLELNDVEIDYCFDCGGIWLDAGELELLMGS
                     SEKKNEILSSFQDVDKTNKEKKRACPICRKPMKKVIIKTENGEVLIDECAEKHGIWFD
                     KGELYQIISCSSEDAGSPVLSFLRDIFCSKHVDNGG"
     gene            711..1289
                     /locus_tag="PL056_08470"
     CDS             711..1289
                     /locus_tag="PL056_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HPM47089.1"
                     /translation="MTAALIFVGALFLLVLFAAVIFIGIYNALVVLRNRVKNSWSQID
                     VQLKRRHDLIPNLVETAKGYMKHENETFVKVTEARSKAMNAGNSGSVKDIGAAEADLS
                     GSLAKLLLTVENYPELKANQNFLALQEELTSTENKIAFSRQAYNDDVMMFNNKIEMFP
                     SSIVAGMFNFEKSEFFEIEDKQEKLAPKVSFN"
     gene            1311..2732
                     /locus_tag="PL056_08475"
     CDS             1311..2732
                     /locus_tag="PL056_08475"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HPM47090.1"
                     /translation="MWELIEQNRRKSKVVFIAMFFLLEALSISIGGAIYYPDGLYWGA
                     LAGFALWAIQSAVAYYFGDAIVLSVSKATKVTKESYPQLYNIVEEMKIAGSQTFMPEI
                     YVINDSAMNAFATGIRPEKSAVCVTAGLLETLNRDELQGVIAHEMSHIINRDVLFMTF
                     AGVMLGSINLIAKGFIVGSRGSLNSRRSRLGSRASNVHPAIMLIAIAFAIIAPILAQL
                     FYFAISRKREYLADATAVRLTRYPEGLASALEKISGSAANLVFYNRITAPMFIINPMA
                     LADEGSLFSTSTHPPTHQRISILRALQNGGKVSFRDYQQQFTKTANERKLLPDSALKD
                     KGEVQVRAGLSPEEVLTAATVASPRNISDLIMATSGFLFMPCACGLKIKVPPTIGKSQ
                     VQCPRCGRYLTVPSVTSGINHSEEKTKPVDGALSVFKRVNPDSWESCLCSCGHTIQLS
                     PIFRGRQVICKKCKNKIQIDYPE"
     gene            2748..4016
                     /gene="tyrS"
                     /locus_tag="PL056_08480"
     CDS             2748..4016
                     /gene="tyrS"
                     /locus_tag="PL056_08480"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017756139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HPM47091.1"
                     /translation="MSVYNELKDRGFISNVTDPEIEKYLNENKVTVYCGFDPTAESLH
                     VGNLVPLMGLAHFQRHGHKVLPLIGGATGMIGDPSGKSEERNLQSITQVMHNADSIKG
                     QMEKVLHFKGENSAFMVNNYDWTHGMSIIDWLRDIGKHFTLSYMLAKDSVKSRLASEN
                     GISYTEFSYMTLQSYDFLYLFRNFGCTMQFGGNDQWGNITAGIELVRRADAGKVFGMT
                     FPLMTTSTGEKFGKSAGNAVWLDPEKTSPYQYYQYWINTTDEDVEKMMKLFTFIDVSE
                     IEKVCGEHKLQPHLRKAQTLLAEEATKILHGEEGLKSAQAASAALFGGDLKGLSEKEL
                     KSIFNDVPSTVVPREEIKNGINIADLLVNTNMQPSKGKARNLIQQGGCYINNEKAEDP
                     EFIVNTSSLMHKSMIIIRCGKKNYHLVNVK"
     gene            4106..6178
                     /locus_tag="PL056_08485"
     CDS             4106..6178
                     /locus_tag="PL056_08485"
                     /inference="COORDINATES: protein motif:HMM:NF039815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47092.1"
                     /translation="MNIRFFSIVLIASLFLFLSASCSVEDSDDLSDEINSGTDSDGNG
                     ALPDNSAGDEQILPGDENGLPDNEDPDDDNVIPGVDSDGDGIPDDVEKPNGVAVDTDE
                     DGDPDYLDTDSDGDGIPDSVEGVIDTDGDGTSDYRDTDSDGDNIEDSVECPSQPCIDT
                     DSDGTPDFLDEDSDGDGISDLVEGTKDPDEDGIPNYLDDDSDGDGIPDSTEGNKDSDE
                     DGFPDYLDLDSDNDGLSDSKELELGTDPTKADTDGDGFDDNTEIAAGSDPLKKDPEFY
                     EGQFYVVLPYMDAEKNDLLEFDTKIKKADILFHFDLTGSMASAIDTVKQDISSIIIPG
                     IQAVLDDPAFAIASYKHIESTPHVMNQPVTTDVAKLQAALNNMTAPGSGSIDSQYETL
                     FQGASGHGFDGELLKFDEGDCFDNALLCLYNYDQGNMDVYYDPYHTVTLASQNCTGQI
                     GHIGGGCFRDAALPIQILMTDEALYDVNEDILSDKGITAYNYKWNSSKPDQGHYREEV
                     KKAMNAVNSKFIGIMGTSLTSELLDIIQDRMTEIADDTDSKTASGEHFIFQVGYTGGG
                     LSNEMVTAINDLLLNIKRDITTQKTSVANSFSIDTTQFIKKIKPDSSIPPNNYDSLDE
                     EWFYNVRPNTNILFDVTFENTIFEPTTTENTLFRARIHVYGEGAILDTRDVFIIVPGI
                     KDDGGIQS"
     gene            6178..6906
                     /locus_tag="PL056_08490"
     CDS             6178..6906
                     /locus_tag="PL056_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47093.1"
                     /translation="MNKFSKLSKLIDNFESQVEFWKRENPGLLEKLKSVNVEVCNGEN
                     GYRIDNAIVFNRNLRKLSETGPKYIIVADNPGMDEQKEENSCYLIGKAGQMTRNFFIN
                     NKLVEDFDSEVAVLNKTCIHTKSTVDLKKLKSFKHLIDETQIFMADIAVDMQKIFDSK
                     LWVIGCSELKEKGIFAAYLKRLKERYENDAESHRDQLFFYPHFSYGNFQKNLNAVLSQ
                     QPELTVEKALVQAGIKINYSEVKK"
     gene            6903..7907
                     /locus_tag="PL056_08495"
     CDS             6903..7907
                     /locus_tag="PL056_08495"
                     /EC_number="6.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase (ATP)"
                     /protein_id="HPM47094.1"
                     /translation="MRKLATIRKVDAIKPIDGADAIELAVFGGWQVVVKKATEIKAGM
                     LVVYCEIDSVFPEKPEYEFLRQDKFRLRTKKFRGALSQGLVFKVEEVLKDGDFKEGDD
                     VTELLGITLYEPPIPAAISGEVAGAYPSSVPKTDEERVQNIDVAEFYDIDFFVTEKLD
                     GTSCSFILDSNGFHVCGRNWEYLEALEQTFWKLAKFYEIESKLKKYYDNTGSYLALQG
                     EVIGNGIQSNVYNLKGQDIFIFNIFDITKQRYLSSTEYRKVCEESGLKTVPVVEEKMT
                     IKGKSHDDFLKFAEGKSLLNPQAEREGVVFRSFDQTERNQGKISFKAISNKYLLKHGG
                     "
BASE COUNT         2474 a         1471 c         1857 g         2107 t
ORIGIN      
        1 ttctcacgat ccctgccggc agagcacctt ttatgaaatc gttagtgttt tcaatgttct
       61 gaaaggtctt tccaaacaaa ttgttgtgca ccggctcatt cttgctcata aacatctcct
      121 aattagaaaa attacaaagc taatgcacaa taaacaatgc ggatgaatta gtcaagaaaa
      181 tgatcggatg gtttctgggg tctttgaagg aattcagcat ccgcctcaat aaaattgaaa
      241 aaaaacctgt tacagttatc atacgctgta ttttttaaga tggagatccg atgttaaggt
      301 gtccggtttg tgcgaaagtt gatatggctg tgcttgaact taacgatgtt gaaatagact
      361 attgttttga ctgcggcggg atatggctgg atgccggaga acttgaactt ttgatgggct
      421 cttcagaaaa aaagaacgaa attctttcgt cttttcaaga tgttgacaaa actaataaag
      481 aaaaaaagag agcatgtccg atatgcagaa agccgatgaa aaaagtgata attaagacag
      541 agaacggtga agttctcatt gatgaatgtg ctgaaaagca cggcatctgg ttcgataaag
      601 gtgaattgta tcagataata agctgttctt cggaagatgc cggaagtccg gtactttcgt
      661 tcttaaggga tatattctgt tcaaaacatg tagacaatgg aggataacag atgacagcgg
      721 cacttatttt tgtaggagca ctttttttac tggttctttt tgcggcagta attttcatcg
      781 gtatctacaa tgctctggtc gtacttcgca acagggttaa gaattcatgg agtcagattg
      841 atgttcagct taaaagaagg cacgatctca tcccgaacct tgttgaaact gcaaaaggtt
      901 acatgaagca tgaaaatgaa acattcgtga aagtaacaga agcaaggtct aaagcgatga
      961 atgccggaaa cagcggttca gtgaaagata tcggagcggc tgaagcggat cttagcggtt
     1021 cacttgcaaa actgcttctt actgttgaga actatccgga attaaaggcg aatcagaact
     1081 ttctcgcttt gcaggaagag cttacttcaa cggaaaacaa gatcgctttt tcaagacagg
     1141 catataacga tgatgtcatg atgttcaaca ataaaattga aatgttccct tcaagcatag
     1201 ttgccggaat gttcaatttt gagaaatcgg aattcttcga gatcgaggat aagcaggaaa
     1261 aactggctcc caaagtaagc ttcaactgat cggattaact ggtgttttag atgtgggagc
     1321 tgatcgaaca gaacagaagg aagtctaagg tcgtattcat tgcgatgttc ttccttcttg
     1381 aagcactttc gatttcgatc ggtggcgcta tctattatcc tgacggtttg tactggggtg
     1441 ctcttgccgg atttgcattg tgggcaatcc aatccgctgt cgcatattat ttcggtgatg
     1501 cgatagttct cagtgtcagc aaagcgacaa aagtgacaaa agagtcgtat ccccagcttt
     1561 acaacattgt ggaagagatg aaaatagcgg gaagccagac attcatgccg gagatatatg
     1621 tgataaacga ttcagcaatg aatgccttcg caacgggaat aagacctgaa aaatcggcag
     1681 tctgcgttac tgcagggctt cttgagacac tcaacaggga tgaacttcag ggtgtcatcg
     1741 cacacgaaat gagccacatc ataaacaggg atgtcctttt tatgacattt gccggagtga
     1801 tgctcggaag cattaactta atcgcaaaag gtttcattgt cgggtcaaga ggttctctga
     1861 attcaagaag gagccgtctc gggtcaaggg cctcaaatgt tcatcctgca attatgctga
     1921 tcgcaatagc ttttgcgata attgctccga tcttagccca gcttttctat tttgctatct
     1981 caaggaagag ggaatatctt gccgacgcga cagcagtccg gcttactaga tatcctgaag
     2041 gtcttgcctc cgcacttgaa aaaatatcgg gttcagcggc gaatcttgtt ttctataaca
     2101 ggataacagc cccgatgttc ataatcaacc cgatggctct tgctgacgag ggctctcttt
     2161 tttcaacatc aactcatcct ccgacacatc agaggatctc tattttaagg gctttgcaga
     2221 acggcggaaa agtgtcgttt cgggattatc agcagcaatt cacaaagacg gcaaatgaga
     2281 gaaaacttct gcctgattct gccttgaaag ataagggtga ggttcaggtg agagcaggtc
     2341 tttcccctga agaagtgtta acggcggcaa cggtcgcttc acccagaaac atcagcgatc
     2401 tgataatggc gacaagcggg tttctgttca tgccgtgtgc ctgcggactg aaaataaaag
     2461 tgcctccgac aattggaaaa tctcaggtcc agtgcccgag atgcggaaga tatctcacgg
     2521 ttccgtcggt cacttcaggg attaaccact cagaagaaaa aacaaaaccg gttgatggag
     2581 cgttatcggt tttcaagagg gtcaatcccg attcgtggga aagctgtctg tgctcatgcg
     2641 gtcacactat tcagctttcg ccgatcttcc gaggaaggca ggttatatgt aaaaagtgca
     2701 aaaataagat acagatcgat tatcccgagt aatatttgga gagaaaaatg tcagtttaca
     2761 atgaattgaa agatcgcggt tttatttcaa atgtgacaga tcctgaaatt gaaaaatatc
     2821 tgaatgaaaa taaagtgacg gtctattgcg gtttcgatcc tacagcggag agtcttcatg
     2881 tcgggaatct tgttccattg atggggcttg cccattttca gcgtcacgga cacaaagttc
     2941 tcccccttat cggcggcgca acagggatga taggggatcc gagcggaaag tctgaggaga
     3001 gaaatctcca gtcaattaca caggttatgc ataatgctga ttcaataaag gggcagatgg
     3061 agaaagttct ccattttaaa ggtgaaaatt cggcttttat ggtgaataac tatgactgga
     3121 ctcacggaat gtcgataatc gactggctta gagatatcgg aaaacatttc actttaagct
     3181 atatgcttgc aaaggattct gtaaaaagcc gtcttgcttc agaaaacggg atctcttata
     3241 cggaattcag ttatatgacg cttcagtcgt atgatttcct ctatcttttc aggaacttcg
     3301 gctgcacaat gcagtttggc ggcaacgatc agtggggaaa tattacggca ggaatagaac
     3361 ttgtcagaag ggcagatgca ggaaaggttt tcggaatgac tttcccgttg atgacaactt
     3421 ccacaggtga aaaattcggc aaaagtgcag gaaatgctgt gtggctcgat ccggaaaaaa
     3481 cgagtcctta tcagtattac cagtactgga taaatacgac ggatgaagat gttgaaaaga
     3541 tgatgaaact gtttacattc attgatgttt ctgaaataga gaaggtctgt ggtgagcata
     3601 agttgcagcc ccatttgaga aaagcgcaga cacttttagc ggaagaagcg acaaaaatac
     3661 ttcacggtga agaaggtctt aaaagtgcac aagctgcaag cgcggcactt ttcggaggtg
     3721 atctcaaggg cttaagcgaa aaagaactca aaagtatttt caacgatgtt ccttcaaccg
     3781 tcgttcccag agaggaaatt aaaaacggca taaatatagc cgatcttctt gtcaacacca
     3841 atatgcagcc atctaaagga aaagcgagaa atcttatcca gcaggggggc tgctacataa
     3901 acaatgaaaa agcggaagac cctgaattta ttgtcaatac atcttcactg atgcataaat
     3961 caatgataat aatccgctgc ggaaaaaaga attatcatct tgtcaatgtg aaatagaaac
     4021 atccaacccg ttgatttttt ttaagtttaa aatacaatat acaacgcatc tgagattttt
     4081 agataaacgg gcgagcgggg gatcaatgaa catcaggttt ttttcaattg ttttaattgc
     4141 gtcactattt ttatttttat ctgcttcatg ttcagttgag gattctgacg atctgtcaga
     4201 tgagatcaat tcaggaaccg attctgatgg taacggagca cttcccgata attcggcggg
     4261 ggatgagcag attcttcccg gagatgaaaa cgggctccct gacaatgagg atcctgatga
     4321 tgacaatgtg attcccggag tggacagtga cggtgacgga atccctgatg atgttgaaaa
     4381 acccaacggt gttgcagttg atactgatga agacggagat cctgattacc ttgacaccga
     4441 tagtgacggt gacggaattc ccgattcagt tgaaggagtt atcgatacgg acggtgacgg
     4501 aacttctgat taccgcgata ccgatagcga tggagataat atagaagaca gtgttgaatg
     4561 tccaagtcag ccgtgcattg atacggatag cgacggaacc cccgattttc ttgatgaaga
     4621 cagtgatggt gacggaatat cagaccttgt tgaagggaca aaagaccctg atgaagacgg
     4681 catccccaac tatcttgatg atgattctga cggtgacggc attcctgaca gcacagaagg
     4741 gaataaagat tcagatgaag acggtttccc cgattatctt gatcttgaca gtgataacga
     4801 tggtttgagt gattccaagg agttagagct tggaaccgac ccgacgaaag cagataccga
     4861 cggagacggc tttgatgaca atacagaaat tgcggccggt agcgaccctc tgaaaaaaga
     4921 tcctgaattt tatgaaggtc agttctatgt cgttcttccg tatatggatg ctgaaaagaa
     4981 cgatcttctt gaatttgata caaaaataaa gaaagcggac atactttttc attttgacct
     5041 taccggttca atggcaagtg cgattgacac agtgaaacag gatatcagca gcataataat
     5101 tccggggata caggcggttc tcgatgatcc cgcttttgcc attgcatctt acaagcacat
     5161 tgaatcaact ccccatgtca tgaatcagcc ggtaacaact gatgttgcaa agcttcaggc
     5221 ggcacttaac aatatgacag ctccgggaag cggatcgatc gattctcagt acgaaacttt
     5281 atttcaggga gcgagcggtc acgggtttga cggagagctt cttaaatttg atgaaggcga
     5341 ctgttttgac aacgcattat tgtgtttgta taactacgat cagggcaata tggatgtcta
     5401 ttatgaccct tatcacaccg taacacttgc ttctcaaaat tgtaccggtc agatcgggca
     5461 tataggcggc ggctgtttca gggatgccgc tcttccaata cagatactga tgacagatga
     5521 agcactttat gatgtcaatg aggatatatt gagtgacaag gggataactg cctataatta
     5581 caagtggaat tcttcgaaac ccgatcaggg gcattatcgc gaggaagtta agaaagcaat
     5641 gaatgccgtc aactcaaagt tcatagggat aatgggaact tcattgacaa gtgaactgct
     5701 tgatattatt caggatagaa tgactgaaat tgcagatgac acagattcaa agactgcatc
     5761 tggagaacat ttcattttcc aggtcgggta cacaggcggc ggattgagca atgagatggt
     5821 gacagcgatc aacgacctgc ttttaaacat caaaagagac attactactc agaaaacaag
     5881 tgttgcgaac agtttcagta tagacaccac acagttcata aagaaaataa agcccgattc
     5941 atcaattccg cccaataatt acgacagctt ggatgaagag tggttttaca atgtccgccc
     6001 taacacaaat attctttttg atgtaacttt tgaaaacacg atctttgaac cgactacgac
     6061 tgaaaacacc cttttcagag cgagaataca tgtttacggt gaaggggcga tccttgacac
     6121 ccgtgatgtt tttatcattg ttccgggcat taaagacgat ggcggaattc agagttaatg
     6181 aataaattca gcaagttgtc aaaactcata gacaattttg aatctcaggt ggaattctgg
     6241 aaaagagaaa accccggtct tttggaaaaa ctgaaaagtg taaatgtgga agtgtgtaat
     6301 ggtgaaaacg ggtacaggat agacaatgcg atagttttca acaggaattt aaggaagtta
     6361 tctgaaacag gtccgaaata tatcattgtt gccgataatc ccggaatgga tgagcagaaa
     6421 gaggagaatt cctgctatct cattggaaaa gcagggcaga tgacacggaa ttttttcatt
     6481 aataataaac ttgttgagga ttttgattcg gaagttgcag tcctgaataa aacctgcatc
     6541 catacaaaga gcaccgttga tcttaaaaag ctcaagagtt ttaagcactt aattgatgaa
     6601 actcagattt tcatggcaga tattgcagtt gacatgcaga agatctttga ctcaaaactg
     6661 tgggttatag gatgcagtga acttaaggaa aaagggatat ttgcagcata cctgaaaagg
     6721 ttgaaagagc gatatgaaaa tgatgcggaa agccacaggg accaactgtt tttctatccc
     6781 catttctcat acggcaattt tcagaaaaat ctcaacgcag tgctgtcaca acagccggaa
     6841 ttaactgttg aaaaagcact tgttcaagca ggaatcaaaa taaattactc agaggtgaaa
     6901 aaatgagaaa acttgcaacg attagaaaag ttgatgcgat taagccgatt gacggcgccg
     6961 atgcaattga acttgcagtt tttggcggct ggcaggttgt cgtgaaaaaa gcgactgaaa
     7021 taaaagcagg aatgcttgtt gtatattgcg aaattgactc agtttttcct gaaaaacccg
     7081 aatatgaatt cttaaggcag gacaagttcc ggctaagaac aaaaaagttc aggggtgcac
     7141 tttcgcaggg gcttgttttc aaagttgaag aggttttaaa ggacggagat ttcaaagaag
     7201 gtgacgatgt gacggagctt cttggcatta ctctttatga accgcctatc cctgccgcaa
     7261 tatctggtga agtcgcagga gcatatccga gttcagttcc caaaactgat gaagagagag
     7321 ttcagaacat tgatgttgca gagttttatg acatcgattt ttttgtcact gaaaagcttg
     7381 acgggacatc gtgttcgttc attctggata gtaacggttt tcatgtctgc ggcaggaact
     7441 gggaatatct tgaggcactg gaacaaacct tctggaagct tgccaaattt tatgaaatcg
     7501 aaagtaaatt gaaaaaatat tatgataata caggcagtta tcttgcactc caaggtgaag
     7561 tgatcggcaa cggaattcaa tcaaatgtat ataatctcaa agggcaggac atttttattt
     7621 tcaacatttt tgacatcaca aaacagcgtt acctttcatc aactgaatat agaaaagtgt
     7681 gcgaagaatc cggattgaaa acagttccgg tcgttgaaga aaagatgacg atcaaaggga
     7741 aaagtcacga tgattttctt aaatttgcag aagggaaatc actgttaaat cctcaagccg
     7801 aaagagaagg agtggttttt cgatcctttg atcaaactga aagaaatcag ggaaagatct
     7861 cattcaaagc gatatctaac aaatatctcc ttaaacacgg tgggtagtt
//
LOCUS       DAOOYB010000131         7786 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_10489_length_7786_cov_10.900272, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000131 DAOOYB010000000
VERSION     DAOOYB010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7786
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10489_length_7786_cov_10.900272"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(137..1168)
                     /gene="mltG"
                     /locus_tag="PL056_08500"
     CDS             complement(137..1168)
                     /gene="mltG"
                     /locus_tag="PL056_08500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HPM47095.1"
                     /translation="MKKSILYIFLIIIVAVLTYTLSKYQDYQDFKIEKVELKDEVTVN
                     IEKGSNWKKVAVTLKGQGLISDDEMFYWMIRENKWGTSLKTGEFSFSGNVSPIDVAKK
                     IMSGKVRLYSFTIPEGYNKFDAAGVFKGIDWIEGSGKFLEICHDKNFLNSIGAKGKQS
                     CEGLLFPSTYHFPKGVDIIAVMTKMADQMKVVLKKYETEMGKLDFDAYDFLKLASIIE
                     KETGVKEEQPMIASVFLNRRRIGMKMQTDPSVIYGLLPKFTGNLTRKDLMTDHPWNTY
                     TRYGFPATPLCFPGEGAIKSIVYPDDTKYLYFVATGKGYHYFSKTLVEHNAAVEHYQV
                     KGLRTKFNY"
     gene            complement(1178..1696)
                     /locus_tag="PL056_08505"
     CDS             complement(1178..1696)
                     /locus_tag="PL056_08505"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011722832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HPM47096.1"
                     /translation="MSEKGFVQVYTGNGKGKTTAALGLALRAAASGKKVFIGQFVKGM
                     HYSELDIIQAIENIEIKQYGADCFIYKDPTENDIKIARDGLAEMADILKNGKYDVVIM
                     DEANIALYFKLFSFEELWEAVLNRAGHVEVIITGRYAPDILIEKADLVTEMKEIKHYY
                     TSGIQARTGIEK"
     gene            complement(1689..2957)
                     /locus_tag="PL056_08510"
     CDS             complement(1689..2957)
                     /locus_tag="PL056_08510"
                     /inference="COORDINATES: protein motif:HMM:NF015882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HPM47097.1"
                     /translation="MLKEKALHYHKFPKPGKIDIVPTKNCISQSDLSLAYTPGVAEPC
                     REIEKDPQTAYDYTGKGNLVAVITDGTAVLGLGDIGPLAGKPVMEGKALLFKRFADIN
                     SYDIEVDQTDPAEFIKIVKALEPTFGGINLEDISSPRCFEIEEKLKEIMDIPVFHDDQ
                     HGTAVIGAAAVLNGLEIADKKPENCRVVLTGPGAAGVAIARHLINCGFMEENIFAYDS
                     KGVLYKGRKDLKSPYHSFLFRDTEKRTLIELFENADVFIGTSVKGMVTEEMITRMAPN
                     PVILPMANPDPEIPYETVVKVRPDAMAGTGRSDYPNQVNNVLGFPAIFRGALDVRARK
                     ITENMKMAATHALMRVAKMPALKSVLDVYGLKELEFGRNYLIPKPFDPRVLVEVAASV
                     GSAAMEEGVSRISIDIEKYKKDLTERFVHE"
     gene            complement(2969..3823)
                     /locus_tag="PL056_08515"
     CDS             complement(2969..3823)
                     /locus_tag="PL056_08515"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HPM47098.1"
                     /translation="MGGMLEILNSYRFVPFTAAFVFIFGLLTGSFLNVCIYRIPLKKS
                     IVFPPSSCTKCSHQIRWYENIPVLSWLFLRGKCSGCGEKISMIYPSIELLNGFLYVLA
                     FVRFGFFPELFLIFYFLSAMIVTAVIDFKTQFVYGGVVLPLIISGMLWSYLYPNATVM
                     YSLTGAMTGGGILLLAIFVFYIVTKKIGMGLGDVYILAAIGAYTGPFRIPLILLAACL
                     TGILYFMFAKLVFKKKRIADNITKEDLNSNDEKDVENAIYFGPFLALAGVTVLLMPPE
                     ILENIFPF"
     gene            complement(3825..5222)
                     /locus_tag="PL056_08520"
     CDS             complement(3825..5222)
                     /locus_tag="PL056_08520"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPM47099.1"
                     /translation="MESNRILVIDDELSMRQFLEILLKKDGYSVDTAENGAKAIEMLK
                     KNKYSVILMDYNMPEAIDGIDLLNELRIRSRKSQIIVITAFASTEQAIKAIELGAVDY
                     VSKPFNVAEIRDIVKKCISIEADEDAHIKEPVTVEKKSRKHESPTAENVVAESSKMLK
                     ILEIAKQIAPTDSTVLLTGDSGTGKEVVARYIHANSGRSNKPWYPVNCGAIPENLLES
                     ELFGHEKGSFTGAYQMKKGYFEVAGDGTLFLDEIGELSENMQVKLLRVLQEKNFTRVG
                     GTEKISTNVRLIAATNRNLKEQMQKGKFREDLYFRLNVLELYIPPLNERRDDILPLAN
                     FFLEQFSRKSGVKYKFSKELIDFFMNYPFPGNVRELKNIIERGTVFAKDGLLTKTHFE
                     NAHKISEKITKDLSIDVSDNIDLDEILAEIEKKYIIAALKKTDYNRHDTCVLLNISER
                     SLRYRISKLGIKDDE"
     gene            complement(5212..6546)
                     /locus_tag="PL056_08525"
     CDS             complement(5212..6546)
                     /locus_tag="PL056_08525"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM47100.1"
                     /translation="MFWVVVSYLSGGIESPYLYVIIINIVYSGILLREKGAIFTTLFA
                     FILLLIQGITIKLSMVPLISTQLVELYASKWETYFSRLFTYLLFFSFTGFVASRISKG
                     FQQASRNLFESAQRNKELRKHFFAIFSNINMGVMILNGLERVYVNKYAQQFETILDEL
                     QKEIMADISAKGRWSEKKINEMWLSYTVMPYVESQSVIIFSDVTSIRKREEELEHSER
                     MASIGKLTASIAHEIKNPLTSLIGASELMFASSGPQIQDQGESVVNDQLIDIIRREGN
                     RVKTLLDSLFRYTEELKYSMSPCGIHDILNDVSILFKLGHPGVAINSNVEDLKIFADA
                     ERMKEVFWNILVNSSEAMAEKGEISVYSKMRNKNVQIIFDDTGPGFIEKNIDRIFDPF
                     FSTKRRGTGLGLAQVYRIVTRHGGSVKAMNTGNGARVIVELTLYKDGDENGK"
     gene            6562..6777
                     /locus_tag="PL056_08530"
     CDS             6562..6777
                     /locus_tag="PL056_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47101.1"
                     /translation="MKICKRFQISLIYASIKDKGNYVDKYNCKRGRKKNLVRKRENVG
                     IFTDERKKKKGKYNSKYEKEPDYKIIV"
     gene            complement(6810..>7786)
                     /locus_tag="PL056_08535"
     CDS             complement(6810..>7786)
                     /locus_tag="PL056_08535"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HPM47102.1"
                     /translation="GLSIIQGLEILAAQSETPAFRSVIRKVKNSVEEGKSLSDALKDH
                     KTVFSDLYVNLVAAGEVGGILDTILDRLAIFLEKNAAINDKIKSAMKYPTVVMIAVIG
                     ITIVLLYWVVPTFAAMFMSQGQQLPGLTQVVVDLSDWTQNNILLIIGALAGIIFGFKA
                     AMKNKQFQFTIHKMSLDAPIVGDLIKKSAVARFTRTLGTLVSSGVPLVDGLEVVSRTA
                     GNLAIEKAIVFVRARVIEGSDMTTPLTDVGIFPSMVVQMIGVGEATGAMDTMLNKIAD
                     FYEDEVDTAIDGLTSMIEPILMIFVGGIVGTMMVAMYLPIFSMGKAVG"
BASE COUNT         2183 a         1784 c         1395 g         2424 t
ORIGIN      
        1 tttatgcctg atgagacccc tgatggtgat gtcagtccgg tatttgtcta ttaaagcttt
       61 gttgcgttga gcacaactca aacggtgtcg ctgagaaaac agcagaagga tttagtctga
      121 attaacagct tttctttcag taattaaact tcgttctgag tcctttgacc tgatagtgtt
      181 caacggcagc gttatgctca acgagcgttt ttgaaaaata gtgatagccc tttcctgtgg
      241 cgacaaaata gagatacttt gtgtcatcag gataaactat gctttttatc gccccttctc
      301 cggggaaaca gagcggtgtt gccgggaatc cgtatcttgt gtatgtattc cacgggtgat
      361 ctgtcatgag atcttttctt gtaagatttc cggtgaattt tggcagtaat ccataaatga
      421 ctgaagggtc ggtctgcatt ttcatcccta ttctacgcct gtttaagaaa acagaggcga
      481 tcatcggctg ttcttctttc acgcctgtct ctttttcaat gatcgaagca agtttcaaaa
      541 agtcgtaagc atcaaaatcg agtttgccca tttcggtttc atatttcttt aaaaccactt
      601 tcatctgatc cgccatcttt gtcattacag caataatatc aactcctttc gggaagtgat
      661 aagttgaagg gaaaagaagt ccttcacagc tctgctttcc ttttgctccg atagaattaa
      721 gaaaattttt atcgtgacat atttcaagaa attttccact tccctctatc cagtcaatcc
      781 ctttaaaaac tccagccgca tcaaatttat tgtaaccctc gggaattgta aatgaataca
      841 gtctaacttt cccgctcatt atcttttttg caacatctat cggactgaca ttgccggaga
      901 acgaaaattc acctgttttt aaacttgttc cccatttatt ttcacggatc atccagtaaa
      961 acatctcgtc atcacttatc aaaccctgtc ctttcaaagt taccgcaacc tttttccagt
     1021 tactcccttt ttcgatattg accgtcacct catctttcag ctcgaccttc tcaatcttga
     1081 aatcctgata gtcctgatat tttgaaagag tataggttaa gactgccaca ataatgatca
     1141 gaaatatgta taaaatgctc tttttcatga atcacctcta cttttctatt ccggttcttg
     1201 cctgaatccc cgatgtgtaa taatgcttta tctctttcat ttcagtgacg agatctgctt
     1261 tttcgatcaa tatatccggg gcatagcggc ctgtaataat gacttcaaca tgtccagccc
     1321 tgtttaaaac agcttcccag agttcttcga atgaaaaaag cttgaaataa agagctatgt
     1381 ttgcttcgtc cattatcaca acatcatatt ttccgttttt gaggatgtcc gccatttcag
     1441 caagtccgtc acgggcgatt tttatatcat tttcagtggg atccttgtaa atgaaacagt
     1501 ccgcaccgta ttgtttaatc tcaatgtttt caatagcttg aataatatca agctcgctgt
     1561 aatgcatccc ttttacaaac tgtccgatga acactttttt ccctgacgcc gcggctctga
     1621 gtgcaagtcc gagtgccgca gtagttttgc ccttgccatt acctgtgtaa acctgaacga
     1681 accctttttc actcatgaac aaatctctcc gtgagatctt ttttatattt ttctatatct
     1741 atgcttatcc tgcttacacc ctcttccatt gccgcacttc caacagatgc cgccacttcg
     1801 acaaggactc tcggatcaaa cggcttcgga ataagataat tccttccgaa ctcaagttct
     1861 ttaagcccgt aaacatcaag aacagatttt aaagccggca tttttgcaac tctcatgagt
     1921 gcatgcgttg ccgccatttt catgttctca gtgatctttc ttgcccgcac atcaagtgct
     1981 cctctgaaaa ttgcaggaaa tccgagcaca ttgttcacct gattgggata atcacttctc
     2041 cctgttccgg ccattgcatc aggtctcacc tttacaacgg tttcatacgg aatttcagga
     2101 tcaggatttg ccatcggaag gatgacaggg tttggcgcca ttctcgtgat catctcttca
     2161 gtgaccattc ccttcacact tgtaccaatg aaaacatcag cattttcaaa gagttctatc
     2221 aatgttcttt tttcagtatc tctgaaaagg aaactgtggt aagggctttt caaatctttt
     2281 cttcccttat acagaactcc tttgctgtca tatgcaaaaa tattttcttc cataaaaccg
     2341 caatttataa ggtgccttgc gatcgcaaca ccagccgctc cgggtcctgt aagaacaacc
     2401 ctgcaattct caggtttctt atccgctatt tcaagaccgt taagcactgc cgccgcacct
     2461 ataaccgctg ttccgtgctg atcatcgtga aaaaccggga tatccatgat ctctttgagc
     2521 ttttcttcaa tttcaaaaca tcttggagaa gaaatgtctt caagatttat ccctccgaaa
     2581 gtgggttcaa gagctttaac tatctttata aattcagcag gatcggtctg atccacttca
     2641 atgtcataac tgtttatgtc tgcaaatctt ttaaagagca gcgccttccc ttccatgacc
     2701 ggcttaccgg caagcggacc gatatctcca agacctaaaa cagcagttcc atcagtaatt
     2761 acagcgacaa gattgccttt tccagtgtag tcataggccg tttgcggatc tttttctatc
     2821 tctctgcatg gctcggcaac tcccggagtg tatgcaagag aaaggtcgct ttgggaaatg
     2881 cagttctttg tgggaacaat gtcaatctta cccggtttag ggaatttgtg atagtgaagg
     2941 gctttttcct taagcatgat ttactccatt aaaaaggaaa gatattttca agaatttcag
     3001 gcggcataag cagaacagta acacctgcaa gggcaagaaa cggaccgaaa tatattgcat
     3061 tttcaacatc tttttcatcg tttgaattta aatcctcttt tgtaatattg tctgcgatcc
     3121 tttttttctt gaataccagc tttgcaaaca taaagtaaag tatcccggtc agacatgccg
     3181 ctaaaaggat cagcggtatc ctgaaaggtc ctgtgtatgc cccgatagct gcaagaatat
     3241 agacatcgcc aagtcccatg ccgatctttt ttgtaacaat ataaaaaaca aatatcgcaa
     3301 gaagcagaat gcctccacca gtcattgcac cggtcaggga atacattacc gttgcgttgg
     3361 gatacagata actccacaac atccctgaaa ttatcagagg aagaacgact ccgccgtaga
     3421 cgaactgagt cttaaaatcg ataaccgctg taacgatcat cgcggaaaga aagtaaaaaa
     3481 ttagaaaaag ttcaggaaaa aatccgaaac ggacaaaagc aagaacatat aggaacccgt
     3541 tcaaaagctc gatcgaagga tatatcatcg aaattttctc accgcatcct gaacattttc
     3601 ctcttaaaaa aagccagctt aaaaccggaa tgttctcata ccatcttatc tgatgactgc
     3661 attttgtgca tgaactcggt ggaaagacaa tgcttttttt taaagggatt ctgtaaatgc
     3721 agacattcaa aaaactccct gtcagaagcc cgaagatgaa aacgaacgcc gctgtaaacg
     3781 gaacaaatcg ataactattc aatatttcta acattccgcc catattattc atcgtccttt
     3841 atcccgagtt tacttattct gtaccttaac gatctttctg aaatgttaag aagaacacat
     3901 gtatcgtgcc tgttgtaatc agttttttta agtgccgcaa tgatgtactt cttctcaatt
     3961 tcagcaagta tttcgtccag atcaatattg tcacttacat caatggacaa atctttcgtg
     4021 atcttttctg aaattttatg agcattctca aaatgagttt tggtaagcag tccgtccttt
     4081 gcaaaaacag tccctctttc gattatattt ttcagttctc taacatttcc ggggaacgga
     4141 taattcatga aaaaatcaat cagttctttg gaaaatttat atttcacacc cgactttctt
     4201 gaaaactgct caagaaagaa atttgcaagg ggaagaatgt cgtcccgcct ctcattcaac
     4261 ggcggaatat agagctcaag aacattgagc cggaaataaa gatcttcacg gaactttcct
     4321 ttctgcatct gctctttaag gtttctgttg gttgccgcaa taagccgtac atttgtggaa
     4381 attttctcag taccgccgac ccttgtaaaa tttttttcct gcagaacgcg gagaagttta
     4441 acctgcatat tttcgctgag ctcgccaatc tcatcaagga aaagagttcc atcccctgca
     4501 acctcaaaat accctttttt catctgatat gcaccggtaa aagacccttt ttcatgtccg
     4561 aacaattcgc tctcaagcag attttcagga attgcaccgc agttaacagg ataccagggt
     4621 ttattgcttc tcccgctgtt ggcgtggata tatctggcaa cgacctcttt tccggttccg
     4681 ctgtcacccg tcaggagaac agtgctgtca gtcggagcta tctgctttgc tatttcaagt
     4741 attttcaaca tcttagagct ttctgcaaca acattttctg ctgtcggact ttcatgtttt
     4801 ctgcttttct tttcaactgt aaccggttct ttgatatgag catcctcatc tgcttcaatg
     4861 gaaatgcact ttttcacgat atcccttatc tctgccacat tgaaaggttt gctgacataa
     4921 tccacagcgc cgagctctat cgcttttatg gcctgttctg ttgatgcaaa agctgttatc
     4981 acgattatct gcgactttct gctccttatc ctcagttcat tcagaagatc tatcccgtca
     5041 attgcctcag gcatattgta atccatcaat attaccgagt atttattttt tttcagcatt
     5101 tctatggctt tggcaccgtt ttcagcagta tcaacactgt aaccatcttt tttgagaagt
     5161 atctcaagaa actgcctcat gctcagttca tcatcaatga caagtattct gttactttcc
     5221 attttcgtca ccgtccttat aaagtgtcag ttcgacaatc actcttgcac cgttaccggt
     5281 attcatcgct tttacagagc caccatgacg ggttactatt ctgtacacct gtgcaagtcc
     5341 caaacctgtc cctctccttt tcgtagagaa gaaaggatca aatattctgt caatgttctt
     5401 ttcaatgaat cccgggccgg tatcatcaaa gattatctgg acatttttat ttctcatttt
     5461 ggaatacacc gatatttccc ctttttccgc cattgcttca gagctgttta caaggatatt
     5521 ccagaaaacc tctttcattc tttcagcatc tgcaaatatc ttcagatctt caacatttga
     5581 atttattgcg acacccggat gacccagctt aaaaagaata gacacatcat ttaaaatatc
     5641 atggataccg catggggaca tactgtattt aagttcttcg gtatacctga aaagtgaatc
     5701 aagaagcgtt ttaaccctgt tcccctctct tctgattatg tctatcaact gatcgttgac
     5761 gacactttcc ccctgatcct ggatctgcgg ccctgaactt gcaaacatca gttcacttgc
     5821 cccgataaga gatgtcagag gattttttat ctcgtgtgcg atcgaagctg taagttttcc
     5881 gatggaagcc attctttcag agtgctccag ctcctcttct ctctttctta ttgatgtgac
     5941 atcagagaat atgataacac tctgagattc gacataaggc attactgtgt aacttagcca
     6001 catttcattt attttttttt cagaccatct tccttttgct gaaatgtccg ccattatttc
     6061 tttctgcaat tcatccagaa tggtttcaaa ctgctgggca tatttattca cataaaccct
     6121 ttccaaaccg ttaaggatca tgactcccat gttgatgttt gaaaatatcg caaaaaagtg
     6181 ttttctcagc tccttgttcc tctgggcact ttcaaaaaga tttctgctcg cctgctggaa
     6241 tccttttgat atcctgctgg cgacaaagcc ggtaaatgaa aagaaaagaa gatatgtaaa
     6301 aagacgggaa aaatatgtct cccattttga tgcataaagt tccacaagct gcgtacttat
     6361 gagagggacc attgaaagct tgatcgttat cccctgtata agaagcagga taaaagcaaa
     6421 aagtgtcgta aatattgcac ctttttctct taaaagtatc cctgaataca caatattgat
     6481 aataattaca taaagatatg gagactcaat gcctccactc aggtatgaaa ccactaccca
     6541 gaacattatt tccagtatga attgaaaata tgcaaaaggt ttcagatatc tctcatttat
     6601 gcttctatta aagataaggg aaattatgtt gataagtaca attgtaaaag aggaagaaag
     6661 aaaaaccttg ttcgcaaaag agaaaatgtc ggtattttca cggatgaaag gaaaaagaaa
     6721 aaaggaaaat acaacagtaa atatgaaaaa gaacctgatt ataaaataat agtataaatg
     6781 attccggaat tcttccgtat agagagggat catccgacag ctttacccat actgaagatc
     6841 ggaagataca tcgcgaccat cattgttccg actattccgc cgacgaagat catgagtatg
     6901 ggctctatca ttgatgtaag accgtcaatt gcagtatcaa cttcgtcttc atagaaatca
     6961 gcgattttat tcagcattgt gtccatcgca ccggtcgcct caccgactcc tatcatctga
     7021 acgaccattg aagggaaaat acctacatcg gtaagaggag ttgtcatgtc acttccttcg
     7081 atgaccctcg cccttacaaa aacgatcgct ttttctattg caagatttcc tgctgtcctt
     7141 gaaacgacct caagcccgtc aacaagagga acacctgaac tgacaagggt tccgagagtt
     7201 cttgtaaacc ttgcgacagc cgatttcttg atgaggtcgc caactatcgg agcatcaagg
     7261 gacattttat gtatagtaaa ctgaaactgc ttgtttttca tcgccgcttt aaaaccgaaa
     7321 attatgcctg ccaaggcccc gataattaga agaatgttgt tctgtgtcca gtcactgaga
     7381 tcaactacca cctgtgtaag tccggggagc tgctgaccct ggctcatgaa catcgctgca
     7441 aatgtgggaa cgacccagta tagaagaact atcgttatgc ctatgaccgc tatcattaca
     7501 actgtgggat atttcattgc actcttgatc ttgtcgttta ttgccgcatt tttttcaagg
     7561 aatatggcaa gacggtcaag aatggtatca agaataccac ccacctcacc tgcggccaca
     7621 aggttgacat aaaggtctga gaaaactgtc ttgtgatctt ttaatgcatc actcagcgac
     7681 tttccctctt caactgaatt tttcactttt ctgatgacgc ttctgaaagc aggggtttca
     7741 gactgtgccg caagtatctc aagaccctga atgatcgaaa gaccgg
//
LOCUS       DAOOYB010000132         7710 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_10668_length_7710_cov_10.019987, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000132 DAOOYB010000000
VERSION     DAOOYB010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7710
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10668_length_7710_cov_10.019987"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            49..1281
                     /locus_tag="PL056_08540"
     CDS             49..1281
                     /locus_tag="PL056_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47103.1"
                     /translation="MKKILMITVFMLFVFNVYGQVYENVERTSSFYLIPVLSDTNERT
                     DGYYLPSLLAANKKGYGYIDCNFTKGGLIVPMMGRTLVAAAWYEHGNTRSMIDIKEIM
                     GDLQPGGPAIPDTNHLMGFAFGANITDKIAIGINFRYLLGRYYEENSTTGAKNSHKID
                     TDRMEINPSLTFRTAKLFADVGLAINFQWMTEKAVGTFEYDRTTTYNGNADFSIFGRA
                     GFKATQYSDIVIGTGFGVLPVHEYVLDNNGDKIAKIQHDSYFWNFKAASVIKPVSWMR
                     IHPALIFSLYHFDNIQKQVDVANPEKMTSKSDEFYLTMALGLDVMPVEWFSIKAGVMK
                     NFKLVDDKTTTVVAETTAQDTALAETLDAYFGPSLTVKGFTFTSLINLKFFTDGPYMI
                     TGNNLSDWAYVATVEYQW"
     gene            1275..2045
                     /locus_tag="PL056_08545"
     CDS             1275..2045
                     /locus_tag="PL056_08545"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPM47104.1"
                     /translation="MVTALVTGASSGFGVDFAHILASKGYDLIIVARRKDKLEEVSRN
                     VADKYGVKVRIIDMDISGFDAAVKLYDLTKDENIEVLINNAGFGMYDLFISQDAAKVD
                     SMIQLNIATLTALTNLFLKDMVKKDSGYILNVASFAGFNPMPSYAAYGGSKAYVMNFS
                     VALSTELKETGSKVKVSAFCPGYTNTEFVAVAGQKRSEFVNRTIGESYPAALEAVNGL
                     FKGKAIVMPRFINKLGALMLKFMSRKKAAETVFKSIRK"
     gene            2042..3235
                     /locus_tag="PL056_08550"
     CDS             2042..3235
                     /locus_tag="PL056_08550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPM47105.1"
                     /translation="MNWLNLFLFISLSISAIIWIWQSYALYMSSRSLPVLEKFNYKSS
                     NPLPKVSIIITACNEEKTIEQAVNSRLSDSYKNIEFIVVNDRSTDGTLQIIQKLAESD
                     PRIKVVNIEILPDGWLGKLHAMQKGVEIANGEWLLFSDADVFISTGALEQVILFAENG
                     SIDHVAVLPDLKSDLFLNNCATSTVLREILLYTRPWTIGRPGAKQAAGSGAFNLVRKT
                     VFDRTNGFHDMKMEVVDDTVLAQQIIEQGGKPSVVIGRTKIGLTWYHSFSELLEGLGR
                     LAFAGLGSFSWKRLAAVASIPFFFDMLPYFGLFLSDSILIKAVSLFTVTLSFIVINLL
                     NIYLKRPLIPTIFPPLNHLLAYFICLHSAIINGIRGGIIWRGTFYPSELLKKGQMFDP
                     MALKR"
     gene            complement(3269..3883)
                     /locus_tag="PL056_08555"
     CDS             complement(3269..3883)
                     /locus_tag="PL056_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47106.1"
                     /translation="MSKAEEGIMFVGPNGSQVCKIIDLSSGKSNFSEEKVKNYIQIKV
                     IDIIKKDLDSKSYNCGSYGLSKNKREHLKNLLEKVSYTAIKSTSKKEHLFFDEYEFES
                     EMVFPEFKNEIESKICKSKKYVWGQEKCLSWEDGAMEIRTKAYSLKIKGKIYEPRFPE
                     IEEKENFVISSIGDSEKYLFTDAVCKDVFLSKDALNYQETVIKK"
     gene            complement(4132..6135)
                     /locus_tag="PL056_08560"
     CDS             complement(4132..6135)
                     /locus_tag="PL056_08560"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HPM47107.1"
                     /translation="MKKMYILLIIIISFNLFGYYSNDKKLEFKNYLKSKNLTELENLI
                     KANGSPDFLFDDEEISPLQYAVHIEDVNLIKLLLRYNANINFRGYEKDPLPSPVFMSI
                     DKGNIEIFKIFAESKSFNIESQENDCTLLLAALHGKQIKIAEFLIEKGADVKKCGNKR
                     TGCPLHLTAVLGLNGIMMEIISKGIDINNEFNKFTPLMYALINGQIEAAAILLKNGAD
                     PYKTFNKENHDIRKLADNSSDELVKKFVNSIFEYKACSRLTSSFEKVNKDESDYRPVR
                     LLDKKTLGIIDNNGRITAKVQANRISYPSNGMVWFENAEPRKYTKKYGFMSVAGKIIS
                     KPVYDDVTQFINEFAWVKKDGRWALIDKNGKVLTQFVFDEPVAITKDFKLGRIGSLLK
                     LVDKENRVLDLKIPEGYELVTPKNYPEVNNGNYLVKKEDHYILTAPSGDKIFENNGEC
                     SLLFEGGVGKFEKKDEHGLFSEEGRIITQFYFESMDEKANNGFFTVSRMLYSMKTQKA
                     FLDPCGNILGSEWFDDVESFAKGGKAIYKKGALWGIIEYTGKKIGQPVYEKIDYTNLS
                     SNNDLIAVKKDGRWGFINSEGEEKISFSFNDAASFKNGRATVLEGNQWWVIDESGKKI
                     FKVSDKGYFQCNLFFDEGSVYNKNGKSLPYIPYYNETIEFGGF"
     gene            complement(6215..7570)
                     /locus_tag="PL056_08565"
     CDS             complement(6215..7570)
                     /locus_tag="PL056_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014958029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPM47108.1"
                     /translation="MFIRNIINELKQWASSGSRKPLILRGARQVGKTTAVKMFAKEFD
                     TFIYLNLEKKSDLEIFQMNLPVEKLMDYVLLTKNIIPKEGRTLVFIDEIQNSPEAVAA
                     LRYFYESLPEYFVIAAGSLLEIIMAKNNISFPVGRVEYRFMYPLTFEEFLVATGSGQA
                     LEFYNAVPCPDFAIPKIMELFHIYTMIGGMPEVVEKYMEKENVSDLRTVYQALFLSYI
                     DDVKKYASEKKEIEIIRHAIESTPFEAGNRIKFQGFGNSNYQSREMGEALRTLSRAML
                     INLIYPITAFHPPALADRKKSPRLQFVDTGLLNFATNSQPYFFKFDDLQNINKGKIAE
                     HIVGQELIGMDSFSPSNYSFWVREKKQSNAEVDFIVQHLHRFIPVEVKSGAEGTLRSL
                     HQFMDSCDHPYAVRLYSGKMSVEEVSIPSGRKYKLLSMPYCFAGKINQYLEWFISGNK
                     Q"
BASE COUNT         2343 a         1560 c         1315 g         2492 t
ORIGIN      
        1 tagttgtaat ttctctgcgg aactttttaa ctttctttgg ggggaattat gaaaaaaatc
       61 cttatgatca cagtatttat gctttttgtt ttcaatgtgt acggacaggt ttatgaaaat
      121 gttgaaagaa cttcaagttt ttacctcatt ccggtccttt ccgacacaaa tgaaagaact
      181 gacggatact atctgccttc acttcttgcc gcaaacaaga aaggttacgg ctacattgac
      241 tgcaacttca caaagggcgg actcattgtt cccatgatgg gaagaactct tgttgctgcg
      301 gcatggtatg agcatggcaa cacaagaagc atgattgaca taaaagagat aatgggcgac
      361 cttcagccgg gtggccccgc aattcccgac acaaaccact taatgggatt cgcattcggc
      421 gcaaacataa ccgataagat cgctatcggc attaacttca gatatcttct cggcagatat
      481 tatgaagaga acagcacaac cggagcgaag aacagccata agatcgacac agatagaatg
      541 gagatcaatc cgtcattaac tttccgtact gcaaaacttt ttgccgatgt aggtcttgcc
      601 atcaatttcc agtggatgac tgaaaaagct gtcggaactt tcgaatatga cagaactacg
      661 acatataacg gaaatgccga tttttcaatt ttcgggagag ccggtttcaa agctacacag
      721 tattcagaca tcgttatcgg aacaggtttc ggagtgcttc cggtacatga atatgttctt
      781 gacaacaatg gcgataagat agcaaagatc cagcatgatt catatttctg gaacttcaag
      841 gcggcatctg tgatcaagcc ggtaagctgg atgagaattc accctgcatt gatcttttcc
      901 ctttaccatt ttgacaacat tcagaaacag gtcgatgttg caaaccccga aaaaatgacc
      961 tccaagagcg atgaattcta tcttacaatg gctctcgggc ttgatgtaat gcctgtcgag
     1021 tggttctcca tcaaggcagg tgtaatgaaa aacttcaaac ttgttgatga taaaacaaca
     1081 acagtcgttg cagaaacaac cgctcaggat actgctctgg ctgaaacact tgatgcatat
     1141 ttcgggccgt cattgactgt aaaagggttt actttcactt ctctcataaa ccttaaattc
     1201 ttcacagacg gaccttatat gatcacagga aataatcttt ctgactgggc ttatgttgca
     1261 actgtggaat atcaatggta acagccctcg tcacaggcgc ttcaagcgga ttcggtgttg
     1321 attttgccca cattcttgct tcaaaggggt atgatctcat aatcgttgca agaagaaagg
     1381 ataagcttga ggaagtcagc agaaatgttg cagataaata cggtgtcaaa gttcgtatca
     1441 ttgacatgga catttccggc tttgatgcgg ctgtgaaact ctacgatctg acaaaagacg
     1501 aaaatattga agtgcttata aacaatgccg gattcgggat gtatgatctc ttcatttctc
     1561 aggatgccgc aaaagttgat agcatgatac agcttaacat tgcgacactc accgctctta
     1621 caaacctttt cttgaaggat atggttaaaa aagattcagg ttatatcttg aatgtggcat
     1681 cttttgccgg attcaatcca atgccaagct acgccgcata cggtggctca aaagcatatg
     1741 tcatgaactt ttccgtggct ctttcaacag aacttaagga aactggctca aaagtgaaag
     1801 tgagtgcctt ctgtccggga tatacaaaca ctgaatttgt tgccgttgcc ggacagaaaa
     1861 ggtctgaatt cgttaaccgg acaattggcg aaagctatcc tgccgcactt gaagcagtta
     1921 atggactttt taagggcaag gcgatagtca tgccgagatt catcaataaa ctcggtgcgt
     1981 taatgctaaa gttcatgagt cgcaaaaaag ctgcggaaac ggttttcaag tccatcagaa
     2041 aatgaactgg ctcaatcttt ttctattcat atctctttct atttcagcaa ttatctggat
     2101 ctggcagagt tatgcattat atatgtcatc aagatcactt cctgttcttg aaaaattcaa
     2161 ttataaatcg tcaaacccac tcccgaaagt aagcatcata attaccgcct gcaatgagga
     2221 aaagacaatt gagcaggcag taaattcaag gttgagcgac agctataaaa acattgagtt
     2281 tatcgttgtt aatgatagaa gcaccgatgg aactttgcag ataattcaga aacttgcaga
     2341 aagtgacccc cggataaaag ttgtcaatat cgaaatactt cctgatggat ggctcggaaa
     2401 gcttcatgcg atgcagaaag gtgttgaaat cgcaaacgga gagtggcttc ttttcagtga
     2461 tgctgatgtt tttatttcaa ccggcgcatt ggaacaggtt atattgtttg ctgaaaacgg
     2521 ctcgattgac catgttgcag tacttcccga ccttaaatca gatctttttt taaataactg
     2581 tgcgacttca actgttctgc gggaaatact tttatacacc agaccatgga cgataggaag
     2641 accgggagca aagcaggcgg ccggatcagg agctttcaac cttgtgagaa aaactgtgtt
     2701 tgacagaaca aacggatttc acgacatgaa gatggaggtt gttgatgaca ctgttctggc
     2761 tcagcaaatt atcgagcagg gaggaaaacc ttcagttgtg atcggtagaa caaagatagg
     2821 tttaacatgg tatcattcat tttcggaact tctggaaggt cttggacggc ttgcctttgc
     2881 aggacttgga agtttttcat ggaaacggct tgccgcagtt gcatccatcc cgttcttttt
     2941 tgacatgctc ccctactttg gcttattttt aagtgattcg atcctgataa aagccgtctc
     3001 cctttttacc gtgacacttt cattcatcgt cataaatctt ttaaatattt acctcaaacg
     3061 acctctgatc cccaccatct ttcccccgtt aaatcatctg cttgcatatt tcatctgtct
     3121 tcattccgca attataaacg gaatcagggg cggaataatc tggagaggaa cattctatcc
     3181 ttcagaactt ctcaaaaaag gacaaatgtt cgatcctatg gcgttgaaac ggtaagatga
     3241 agcgactgag acatcaataa taaactagct attttttgat gacggtttcc tgataattca
     3301 acgcatcttt tgaaagaaaa acatctttgc atactgcatc tgtgaataaa tatttttcac
     3361 tatcaccgat actgcttatt acaaaattct ctttttcttc tatttcagga aatcttggtt
     3421 catagatttt cccttttatt ttaagagaat atgcttttgt tctgatctcc atggctccat
     3481 cctcccaaga aaggcatttt tcctgccccc agacatattt tttgctttta cagatttttg
     3541 attctatttc gtttttaaat tctgggaaaa ccatttctga ttcaaattca tattcatcaa
     3601 agaatagatg ctcttttttc gatgtgcttt taattgctgt ataagatact ttttcaagaa
     3661 gatttttgag atgttccctt ttgtttttac ttaaaccata agatccacag ttgtatgatt
     3721 ttgagtcaag atctttcttg attatatcaa ttacttttat ttggatataa ttctttactt
     3781 tttcttcaga aaaattcgat tttccagaac ttaaatcaat gatcttgcaa acttgagaac
     3841 catttggacc gacaaacata atgccttctt cagcttttga cacagaaatt gtcgatccga
     3901 aaacagatgt tgtatataca ttttcatttt tcactaatga aagtgtattt cttgcatctg
     3961 ataattctgt ggcaggagaa attttcacta ttttaagaac tgtctgttca ttttcctttt
     4021 taattgacat taattctttg tcacacagaa aatttccctc aatgttttta ctgcaaccca
     4081 aaaaaaggaa gaaaaacaac attaaaaatc caattttaac agacactttc attaaaaacc
     4141 tccgaactct atggtttcat tataatacgg tatatacgga agagatttac catttttgtt
     4201 gtaaacagat ccttcgtcaa aaaaaagatt acattgaaaa tatcctttat ctgacacctt
     4261 gaatatcttt ttgccgcttt catctattac ccaccactga tttccttcaa gcactgtggc
     4321 tctcccgttt ttaaaagaag cagcatcatt aaacgagaat gagattttct cttctccttc
     4381 agaattaata aatccccacc ttccatcttt ttttacagca attaaatcat tatttgatga
     4441 caagttagta taatcaattt tttcatacac cggctgtccg attttttttc ctgtatattc
     4501 aattattccc cataaagcac cctttttgta aatggctttc ccgccttttg caaacgattc
     4561 aacatcatcg aaccattcgc ttccaagaat atttccgcat ggatctaaaa atgccttttg
     4621 tgttttcata ctgtaaagca ttcttgagac agtaaaaaaa ccgttgttgg ctttttcatc
     4681 catggattca aaataaaatt gtgtaattat tcttccttcc tcagaaaaca aaccatgttc
     4741 atcttttttt tcaaactttc ctacaccgcc ttcgaacaat aatgaacatt ccccattatt
     4801 ttcaaaaatc ttatcacctg aaggagccgt taaaatataa tgatcttctt tcttaaccaa
     4861 atagtttcca ttattcactt caggataatt cttaggagta acaagttcat atccttccgg
     4921 tattttaaga tctaaaactc tgttttcctt atctacaagc ttcaataagg aaccaatccg
     4981 acccagtttg aagtcctttg tgattgcaac aggttcatca aaaacaaatt gagttaaaac
     5041 tttcccgttt ttatcaatca atgcccatcg tccatctttt ttaacccatg cgaattcgtt
     5101 aataaactga gtgacatcat cataaacggg tttcgaaatt atcttgcctg caacgctcat
     5161 gaaaccatat tttttagtgt atttcctggg ctctgcattt tcaaaccata ccatcccgtt
     5221 tgatggataa gatattctat ttgcctgaac tttagctgtt atgcgaccat tattatcaat
     5281 aatcccgaga gtttttttgt ctaatagcct aacaggtctg taatccgact catctttatt
     5341 cactttttca aaagatgaag tgagcctcga acaggcttta tattcaaaaa tagaattgac
     5401 aaatttctta acaagctcat cgctactgtt gtcagccaat tttctaatgt catgattttc
     5461 tttattaaag gttttgtatg gatcagctcc attttttaaa agaattgctg ctgcttctat
     5521 ctgtccgtta attaaggcat acatcaacgg agtaaattta ttaaattcat tattaatatc
     5581 aatgcctttt gatattatct ccatcattat accatttaat cctaaaaccg cagttaaatg
     5641 tagtgggcaa cccgttcttt tatttccgca ttttttaaca tcagctcctt tttctatcaa
     5701 aaattctgct attttgattt gctttccgtg tagagccgca agcaagagag tgcaatcatt
     5761 ttcctgactc tctatattga aagatttgct ttcggcaaaa atcttaaaaa tttcaatatt
     5821 tcctttatct attgacataa ataccggaga tggcaaagga tctttctcat aacctctgaa
     5881 gtttatattc gcattgtatc gaagtaaaag cttaatcaaa ttaacatctt caatgtgaac
     5941 tgcatattga agcggagata tttcttcatc atcaaataaa aaatcaggag aaccgtttgc
     6001 cttaataaga ttttccaact ccgttaagtt cttgctttta agataatttt taaactccaa
     6061 ttttttatca ttggagtagt aaccaaacaa attaaaagaa attataatta tcaacaagat
     6121 gtacatcttt ttcataaatt cctccttttg aaaaagtagg ttcagaatat aaaatcatga
     6181 gattttttca acaaaatttt gcgactaaca aaaactactg cttatttcca gaaataaacc
     6241 attcaagata ttgattaatt tttccggcaa aacaatacgg catactaaga agtttatatt
     6301 ttctgcctga tggtatgctg acctcttcga cagacatttt tccgctgtac agccgtactg
     6361 cataaggatg gtcacaggaa tccataaact gatgaagcga tcttaatgtc ccttcagctc
     6421 ctgatttgac ttcaaccggg atgaatctgt gaagatgctg aacaataaaa tcgacttcag
     6481 cgttggactg ttttttttca cgcacccaga acgaataatt tgaaggggaa aaactatcca
     6541 ttccgatcag ttcctgcccg acaatatgct ccgcaatttt tcctttattt atgttttgaa
     6601 gatcatcaaa tttgaagaaa taaggttgtg aatttgttgc aaaattcaac aatcctgtat
     6661 ccacaaactg aagccgtgga gatttttttc tgtcagccaa cgcaggtgga tgaaaagctg
     6721 ttatcggata tatcaggttt atcagcattg ctcttgaaag ggttctgagt gcttcaccca
     6781 tttctctgct ctgatagttg gaatttccaa atccctgaaa cttaattctg ttcccggctt
     6841 caaaaggtgt actttcaatg gcatgacgga ttatctcaat ttcctttttt tccgatgcat
     6901 attttttaac atcatcgata taacttagaa aaagtgcctg ataaacagtt ctcaaatcgg
     6961 aaacattttc cttttccata tatttttcaa ccacttcagg cattcctccg atcattgtat
     7021 atatatgaaa aagttccata attttgggaa ttgcaaaatc aggacatgga acagcattat
     7081 aaaattccag tgcctggcca ctgcctgttg caacaagaaa ttcttcaaaa gtgagtggat
     7141 acataaaacg gtattccact ctgcccacag ggaaacttat gttgtttttt gccataatta
     7201 tctcaaggag ggagccggcg gcaatcacga aatattccgg cagtgattca taaaaatatc
     7261 gaagtgcagc aaccgcctca ggactgttct ggatctcatc aatgaaaaca agcgtcctgc
     7321 cttcttttgg tattatgttc ttagttagaa gaacatagtc catcaatttt tctacaggca
     7381 gattcatctg aaaaatctca agatcgcttt ttttctcaag attcagataa ataaaagtgt
     7441 caaactcttt tgcgaacatt tttacagcag tagttttccc cacctgtctt gcacctctta
     7501 aaataagtgg ctttcgacta ccgcttgaag cccattgctt taactcattg ataatatttc
     7561 ttataaacat taattctcca tcacaaaaaa cacaaagtca gcccaaatat aacccgcatc
     7621 tgacacaaag tcaatccatt ttttaatatt ttctgacaca aagtcagggc agattcaaaa
     7681 atggaaaaaa ctgcattatc aactatgatg
//
LOCUS       DAOOYB010000133         7701 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_10697_length_7701_cov_10.291656, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000133 DAOOYB010000000
VERSION     DAOOYB010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7701
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10697_length_7701_cov_10.291656"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1288
                     /locus_tag="PL056_08570"
     CDS             <1..1288
                     /locus_tag="PL056_08570"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPM47109.1"
                     /translation="LPFALTYAQKRVLEEIRNDLRSVRPMNRLLQGDVGSGKTAVMIL
                     SGLDVISSGYKTVIMAPTEILAVQHFNTISKNVPESLKIELFLGGVTGKKKKAQRAEN
                     AAKADFIIGTHALFENMEFMNDIGLVIIDEQHRFGVSQRMSLQNKAMNPDVLIVSATP
                     IPRSLALTVYGSTDISVIDEMPPGRKPIVTRHVKHFNRNKVFDHVVEIIEKNNEKGYW
                     VCPLVEESEKIDLKHVEGVFEEFRAVLGERVFLIHGKMKGDEKSAAIEKLKSGVANLL
                     VSTIVVEVGVDVTDASFMVIENAERFGLAQLHQLRGRVGRGEKKSFCALISSEEVGGK
                     ADKRLEFIAKTENGFKIAEYDLKTRGPGAMTGLEQSGFRNDPYFLLAAVHGDLVERAG
                     SAVKKIFAGKNIEMIKYLEKVFDQFFKESFNRFKTG"
     gene            1242..1853
                     /locus_tag="PL056_08575"
     CDS             1242..1853
                     /locus_tag="PL056_08575"
                     /inference="COORDINATES: protein motif:HMM:NF024952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HPM47110.1"
                     /translation="MISFLKKVLTGLRPVRYRSASKTLAVLGYFDKKTEEMICDVKKR
                     TELSICELGETVYSSPHITFLITDDRGIDKMKKQLDGITRKVRLPVVRFSHIGYFPSS
                     GVVFIGITPTTELMRFHTVICSKFEGTDSRISPYYMPSKWIPHCSIADGVEEISIKTS
                     VYREIPFPFEARIERLAITEMDHSTGKLVNSVDIQEKVFPDQD"
     gene            complement(1774..3141)
                     /locus_tag="PL056_08580"
     CDS             complement(1774..3141)
                     /locus_tag="PL056_08580"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HPM47111.1"
                     /translation="MILSVLIFAFILIRDHNTPVSLTNSIEPPQWIQLLKNDLAKNSQ
                     SLKNREFILALATGERKFSPQFRNDLINTGTMHLVAISAFHAGIMVILFNFLFRFLII
                     FLPFRYRTSSAILFLLKITASFFYFFITGGSIPTLRALSFILFFDMFLLKGYFPQPLF
                     LFIISITTVAVIIPHSITSLSFIMSAICVATVLQIYKVLPPSVTLKLICVSAVLNYAL
                     LPVYSIISGTFPLMAPLVNLFVIPVVSISIPFITLAQFTIPFSVSISAFFLHLSDIII
                     EPAHFMISFLGRASSDSLIPLIDPPFFIKIIFVTTFFLSIYLRKKAKTVAVITNAALL
                     PFFAFNFQIDQFTIVRSVQLFEKAYCITYPGNSGRMFFDRYRYNPKFNSFLLSRMETA
                     ASACGVIDVISIHTPHKLSKEIQDRIRKKRRFSNVRFFNLDPETPFPECQRCLPAFRY
                     YDPFP"
     gene            3342..4694
                     /locus_tag="PL056_08585"
     CDS             3342..4694
                     /locus_tag="PL056_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HPM47112.1"
                     /translation="MKFRKFDGSFDSIGFLSLLMLDINGTIRQVTVPKGYINEKVLEK
                     GIGFDASNFGYAKVHKSDMVAKPDMLTAFVEEKEGHTILHAFCDVYLTDGTEFDQYPR
                     AVAKKTVEYLQKEGIADDAKVLVELEFHVFDTVKYSTDFSHSFYEVQSSEGIGPDFED
                     DPRFHIQKGYHRLYPADKSFNLRNDIVAALESAGIPVKYHHHEVSAAQLEIELDFISL
                     AQAGDKVSLAKWIAVNVAREHGVFITFMPKPVYNIAGNGMHVHQFLTKNGKSFFAGDG
                     MHGLSKESLAYTAGILDHSLTGSLLAFSNPSTNSYKRLVPGFEAPVGATFAKGSREAS
                     IRIPAYLAKGEERIEYRTGDATANIYYFLCAMILAGVDGIKKGRDPVESNYHDRENGK
                     TFPLNLNSVLDGLLNDNAYLLPAFPETLINYWVKTKRAEAQYVYNAPTPQEYELYFNI
                     "
     gene            4889..6094
                     /locus_tag="PL056_08590"
     CDS             4889..6094
                     /locus_tag="PL056_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPM47113.1"
                     /translation="MTQFKQIYVRLMNGMDIKTHRYLYSKLLPDERIVGVAGQRGVGK
                     TTMMLQYLLENGYNTQNSLYFSADHIYFSEYRLYDYIEKKFLEEGIELFFIDEIHRYT
                     GWSQELKNIYDSFPKIRIVFSGSSMLDLIKGEYDMSRRALLLHLEGVSFREYLSMKNI
                     FKTDPFTMEELLNDSVGISEKLSTIPMISGHFKDYLKYGYYPFSINNVESFSAKLLSI
                     IDKTIQIDIAGFYNLKTQNLSLFKQILLFLATNPPGEFNIHNLAQSLKIDDKTVVNYL
                     EILIETGLVTPLFYNSGGHKMLRKPVKYYLNNSSLYFTLAMESGHEIEIGALRESFFL
                     SMVKNAGLTPHYSSKGDFEVKGFTFEVGGKNKKRKQIKDVERSFVVKDDVLIAQKGEI
                     PLYLFGFLY"
     gene            complement(6108..6530)
                     /gene="fabZ"
                     /locus_tag="PL056_08595"
     CDS             complement(6108..6530)
                     /gene="fabZ"
                     /locus_tag="PL056_08595"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HPM47114.1"
                     /translation="MLNSEQIKKILPHRYPFLMIDRAEVIEEGKKAAGCKMVSVNEPF
                     FQGHFPQEAVMPGVLIVEAMAQLGAVAVLSMEQFKGKIAYFTGINNAKFRQKVVPGDK
                     LDLEIEIVKMRHSVGIGAGKAFVNGILVCEAEISFAIG"
     gene            6743..>7701
                     /locus_tag="PL056_08600"
     CDS             6743..>7701
                     /locus_tag="PL056_08600"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HPM47115.1"
                     /translation="MTANFKAAITGTGSFVPEKIMTNFDLEKIVDTSDEWIRTRTGIS
                     ERRIADENTATSDIASIAAMRAIEDAGLTSEEIDLILVATVTPDMSFPSTACFVQKNI
                     GAVNAAAFDMGAGCTGFIYGLSVANSFITAGIYKNILVIGAETLSKVINWEDRNTCVL
                     FGDGAGAVVVSRNEGEEGILSVKIGSNGNHTGLITQPGGGSRTPATCEVIEKKLNTIH
                     MEGSEVFKLAVRTMSNVSLEVLEEANLNVEDLTLVIPHQANQRIIDGIFNRLNVPKIK
                     THLNLEKYGNTSAASIPLALDEAARMGMLKKGENLLMVAFGSGL"
BASE COUNT         2409 a         1386 c         1751 g         2155 t
ORIGIN      
        1 tctgccgttt gctttgactt atgctcaaaa aagagttctt gaggaaataa ggaatgatct
       61 tcgatccgtc cgtccgatga accggctttt gcagggtgat gtcggaagcg gaaagactgc
      121 tgtgatgatt ttatcagggc ttgatgtgat aagttcggga tataaaaccg ttatcatggc
      181 gccgacagaa attcttgcgg ttcagcactt caacacaata tcaaaaaatg tccctgaaag
      241 tttgaaaata gagcttttcc tcgggggtgt gacagggaaa aagaaaaaag cgcagagggc
      301 tgaaaatgcg gcaaaggcag attttataat cgggacacac gcattgtttg aaaacatgga
      361 atttatgaat gatatcgggc ttgtgatcat tgacgaacag caccgtttcg gagtttcgca
      421 gagaatgtcg ttgcagaata aggcgatgaa tccggatgtt cttatcgtga gtgcgacacc
      481 cattccgaga tcacttgccc tcacagttta cggttcgacc gatatcagcg tcattgatga
      541 aatgccgccg gggagaaagc ccattgttac gaggcatgta aagcatttca acagaaataa
      601 ggtttttgac catgttgtcg aaataattga gaaaaacaat gaaaaagggt actgggtctg
      661 tcctcttgtc gaagagagtg aaaagatcga cctgaagcat gttgaagggg tttttgaaga
      721 attcagggct gttcttggtg agcgggtgtt tctgatccac ggcaaaatga aaggggatga
      781 aaaaagtgcg gccatcgaaa agctcaaatc cggagttgcc aatcttcttg tttcaactat
      841 tgtcgttgag gtcggcgttg atgttaccga tgcgtcgttc atggtgatag aaaacgctga
      901 aaggttcggg cttgcacagc ttcatcagct cagaggaagg gtcggcagag gtgagaaaaa
      961 gtctttctgt gcccttattt cgagtgaaga agtcggaggc aaggcagata aacggcttga
     1021 atttattgca aaaactgaaa acggatttaa aatcgcggag tatgatctca agacgagagg
     1081 tcccggagcg atgaccggac ttgagcagag cggtttcagg aacgatccat atttcctgct
     1141 tgcggcagtc catggtgatc ttgtcgagag ggcaggaagt gccgtgaaaa agatctttgc
     1201 aggtaagaac attgaaatga taaaatatct ggagaaagtg tttgatcagt tttttaaaga
     1261 aagttttaac aggtttaaga ccggttagat acaggtcggc atcaaaaacg ctcgcagtgc
     1321 tcgggtattt tgacaagaag accgaggaaa tgatctgcga tgttaaaaaa aggacggaac
     1381 tcagcatctg tgaactgggt gaaactgtat attcctctcc gcacataact tttctaatca
     1441 ccgatgacag agggatcgac aaaatgaaaa agcagcttga cgggataacc agaaaagtgc
     1501 gtctgcctgt tgtgagattt tcgcacatcg gatattttcc ttcatccgga gttgttttca
     1561 taggaataac tcctactacc gaactgatga gatttcatac agtcatctgt tcaaaatttg
     1621 aaggaaccga ttcccggatc agcccttatt acatgccgtc aaaatggata cctcattgtt
     1681 cgatcgctga cggagtcgaa gaaatatcaa taaaaacaag tgtgtatcga gaaatacctt
     1741 tcccttttga agcaagaata gagcgtcttg ccatcacgga aatggatcat agtaccggaa
     1801 agctggtaaa cagcgttgac attcaggaaa aggtgtttcc ggatcaagat taaaaaatct
     1861 cacatttgaa aatcttcttt tttttcttat acggtcctga atttcttttg acagtttatg
     1921 gggagtgtgg attgaaatga catcaataac cccgcatgca gaggcggctg tttccattct
     1981 tgacaacaaa aatgaattga acttcggatt atacctgtat ctgtcaaaaa acattctccc
     2041 tgaatttccc ggataggtaa tgcagtatgc cttttcaaaa agctgaacgc ttcttacgat
     2101 ggtgaactga tctatctgaa agttaaatgc aaagaacggg agcaatgccg cattagtgat
     2161 tacggcgact gtttttgctt tttttcttaa atagatggaa agaaagaatg tggttacgaa
     2221 gataattttt ataaaaaacg gaggatctat caacggaatg agcgaatctg aagaggccct
     2281 gccaagaaaa ctgatcataa aatgtgccgg ctctataata atatcagaca gatggagaaa
     2341 aaacgctgaa atgctgactg aaaaagggat ggtgaactgt gcaagagtga tgaacggaat
     2401 tgaaatggag acgacaggta tgacaaaaag gttgactaac ggcgccatta aaggaaatgt
     2461 acctgatatt attgaatata ctggtaacag tgcatagtta agaactgctg aaacacagat
     2521 cagtttcaat gtgacagatg gaggaaggac tttatagatc tgaaggacgg ttgccacaca
     2581 gatcgcactc atgataaagc tcagacttgt gatgctgtga ggaattatca cagcgacggt
     2641 cgttattgaa atgatgaaaa ggaaaagggg ttgaggaaaa tatcctttca gcaggaacat
     2701 gtcgaaaaaa aggatgaacg aaagcgccct gagagtcgga atggagccgc ctgtgatgaa
     2761 aaaatagaaa aatgatgcag tgatcttcaa taaaaaaagg attgctgagg aagttctgta
     2821 acggaacgga agaaaaatga taagaaacct gaacagaaaa ttgaaaagaa tgaccattat
     2881 tccggcatgg aaagctgaaa tagcaacaag gtgcattgtc cctgtgttga tcagatcgtt
     2941 cctgaattga ggagagaatt ttctttctcc tgttgcaagt gcgaggatga attcgcggtt
     3001 tttcaacgac tggctgtttt ttgcaagatc gtttttaagc agttgtatcc attgaggcgg
     3061 ctcaattgaa tttgtcaatg aaacaggggt gttatgatct ctgatgagga tgaatgcaaa
     3121 gatcagaaca gataagatca tgctgtttct gtgtggtttt aaatacgctg caaactgttt
     3181 taatgagaag gtcatataaa aaaaccagag ttttgtcaca gctaatttac atgtttggat
     3241 gacaatcgtc aaataaaaag ttgaaaagta cttgaaataa aaaattttat ttgatagatt
     3301 gtatgtccgc gactggtttt ttttaataat ggaggtgagt tgtgaaattc agaaaatttg
     3361 acggcagttt tgacagtatc ggttttttga gccttcttat gcttgatatt aacggaacta
     3421 tcaggcaggt caccgttccc aaaggttaca taaatgaaaa agtccttgaa aaagggattg
     3481 gctttgatgc atcaaatttc ggctatgcaa aagtccataa atccgacatg gttgcaaagc
     3541 cggatatgtt gacggcattt gtcgaggaaa aggaagggca tacgatactt cacgctttct
     3601 gtgatgtcta tcttactgac ggaactgaat tcgatcagta tccgagagct gttgcaaaaa
     3661 agacagtcga atatcttcag aaagaaggta tagccgatga tgcgaaagtt cttgtcgaac
     3721 ttgaattcca tgtttttgat acggttaagt attcaaccga tttcagtcac agtttctatg
     3781 aagttcaaag ttcggaaggg ataggccccg attttgaaga cgatccgaga tttcacattc
     3841 agaaaggtta ccaccgcctt tatccggcag acaagagttt caatttgaga aatgacattg
     3901 ttgcagccct tgaatcggca gggatccctg taaaatatca tcatcatgag gtgagtgcgg
     3961 cacagcttga gatagaactc gatttcatat cattagcgca ggcaggtgac aaagtttctc
     4021 ttgcaaagtg gattgcagtc aatgttgcaa gggaacatgg cgtatttatt acatttatgc
     4081 cgaaacccgt ttataacatc gcaggcaacg gaatgcatgt tcaccagttc ctgactaaga
     4141 acggcaagtc gttttttgca ggtgatggga tgcacggact ttcaaaagag tcgcttgctt
     4201 atacggcagg tatccttgat cacagcttga ccggatcact tcttgcgttc tcaaatccga
     4261 gcacgaattc atataaacgg cttgttcccg gttttgaagc acctgtcgga gcaactttcg
     4321 caaagggctc aagagaggcc tcgatcagaa ttcccgcata tcttgcaaaa ggggaagaga
     4381 ggatagaata tagaacaggg gacgcaactg ccaatattta ttactttctc tgcgcaatga
     4441 tacttgcagg agttgacgga ataaagaagg ggagagatcc ggtcgaaagt aactatcacg
     4501 accgcgagaa cggaaaaact ttcccgctca accttaattc agtgcttgac ggacttctta
     4561 atgacaacgc ttatcttctt cccgcatttc cagaaacgct cataaattac tgggtaaaga
     4621 caaaaagagc cgaagcacag tatgtctata acgctccaac acctcaggaa tacgaacttt
     4681 atttcaatat ttaaccgata aattcgcctg gcccgatggg cacgggctga ggaaacgcca
     4741 ccctttaggc ggcttgatct ttgcgggctg aaagctcgcg ttatcttagc ccacggttga
     4801 aagcctgggt gattttgaat tcttgacagc cctgaatttt ggagtagaat ccaaaaaagt
     4861 tgattaaatt ttggagtgga gtctaaaaat gacacaattt aagcaaattt atgtcaggtt
     4921 gatgaatgga atggatatca aaacgcatcg gtatctttat tcaaaactcc ttcctgatga
     4981 aagaattgta ggtgttgccg gacaacgagg tgtcggaaaa acaacgatga tgttgcaata
     5041 tttattggag aatggctata acacacagaa ttcattatat ttttctgctg atcatatcta
     5101 ttttagcgaa taccggcttt atgattatat tgaaaaaaag tttcttgaag aaggaattga
     5161 gctttttttt attgacgaaa tacaccgtta taccggatgg agtcaggagc ttaagaatat
     5221 ttacgattct ttcccgaaga tcagaatagt tttttcggga agttcaatgc ttgatcttat
     5281 caaaggggaa tacgatatgt cccgaagagc tctcctttta catcttgaag gcgtttcttt
     5341 cagggaatat ctctcaatga aaaatatttt taaaacagat ccgtttacga tggaagaact
     5401 tttgaatgac agtgtcggaa tttctgaaaa actttccact attccgatga tatcaggtca
     5461 ttttaaagat tatcttaaat atggttacta ccctttttcg attaataatg ttgagagctt
     5521 ttctgcaaaa cttctttcga taattgataa aaccatccag atagatattg ccggttttta
     5581 taatcttaaa acgcagaacc tttcactttt caagcagata cttctttttc tggcaacgaa
     5641 tcctcccggt gaattcaaca ttcataatct tgctcaatct ttgaaaattg atgacaagac
     5701 tgttgtaaat tatcttgaaa tattgataga aaccggactt gtcactcctc ttttttataa
     5761 ttcaggcgga cacaaaatgt tgagaaaacc ggtcaagtat tatcttaaca acagctcttt
     5821 gtattttact ctcgctatgg aatcgggaca tgaaattgag atcggcgctt taagagagtc
     5881 attttttctt tcgatggtga aaaatgcagg actcactccg cactattctt ccaaaggaga
     5941 ttttgaggta aaaggattta ctttcgaagt cggaggaaaa aataaaaaga gaaaacagat
     6001 aaaagatgtc gaaagatcat ttgttgtcaa agacgatgtt cttattgctc aaaaagggga
     6061 aatacccctg tatcttttcg gttttttgta ttgaattatt tcaatattta accgatggcg
     6121 aaactgattt cggcttcaca gactaaaatg ccgttgacga atgcctttcc tgcaccgatg
     6181 ccgactgaat gacgcatttt tacaatctcg atttcgaggt caagtttgtc tccggggact
     6241 actttctgac ggaattttgc gttgttgatc cctgtgaaat aggcgatctt tcctttaaat
     6301 tgttccatac ttaagactgc gaccgctccg agctgagcca tcgcttcaac aatgagaact
     6361 cccggcatga cagcttcttg aggaaaatga ccctgaaaaa aaggttcatt cacggaaacc
     6421 attttgcagc ccgctgcttt tttcccttct tcaataactt ccgccctgtc gatcataaga
     6481 aacggatagc ggtgcggcag aatcttttta atctgttcac tgtttaacat tttcacctcc
     6541 cggttatgaa aaaactaaaa aagaaagatc gtcaactaat atcattacac gcaaaaaatg
     6601 acaagaaatt aattctaaaa taccgatttg atgctaaaaa ccaaataata acaaaaagtt
     6661 gcgagtgata atttttttat ttgattttac tggacaaaaa gatagactga catgtcagtt
     6721 tttttatgat atgaggtata tgatgactgc aaatttcaaa gctgctatca caggaaccgg
     6781 aagttttgtc ccggaaaaga taatgaccaa ttttgatctt gagaaaattg ttgatacaag
     6841 tgatgaatgg atcaggacaa ggaccggtat ttcagaaaga agaattgcgg atgaaaatac
     6901 tgcaacttct gatattgctt caattgccgc aatgagagca attgaagatg caggactgac
     6961 ctctgaagag atcgacctta tacttgttgc aacagtgact cccgatatgt ccttcccttc
     7021 aacggcctgt tttgttcaga aaaacatagg agcagtcaat gctgccgctt ttgatatggg
     7081 agctgggtgt accggtttta tttacggact ttctgttgcc aacagcttta taacagctgg
     7141 aatttacaaa aatattcttg ttataggtgc tgaaactctg agcaaggtca tcaactggga
     7201 ggacaggaac acctgtgttc ttttcggtga tggagcagga gcggttgtgg tctcaagaaa
     7261 tgaaggtgag gaagggatcc tgtcggttaa gatcggttca aacggaaatc ataccggtct
     7321 cattacacag cccggaggcg gttcgagaac gcctgcaaca tgcgaagtta tagagaaaaa
     7381 gttaaataca atacacatgg aagggagtga agttttcaaa cttgcagtaa ggacaatgtc
     7441 aaatgtttcg cttgaagttc ttgaggaagc caatctcaat gttgaagatc ttacacttgt
     7501 tattcctcac caggcaaacc agagaataat tgacggtatt ttcaatcggc tcaatgttcc
     7561 aaaaataaag acacatctga atcttgaaaa atatggaaac acttccgcgg cttcaatccc
     7621 gctggctctt gatgaagctg caagaatggg aatgctgaaa aaaggggaaa accttctgat
     7681 ggtcgctttc ggaagcggtc t
//
LOCUS       DAOOYB010000134         7650 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_10783_length_7650_cov_10.600527, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000134 DAOOYB010000000
VERSION     DAOOYB010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7650
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10783_length_7650_cov_10.600527"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..960)
                     /locus_tag="PL056_08605"
     CDS             complement(<1..960)
                     /locus_tag="PL056_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005849227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HPM47116.1"
                     /translation="MSYRPPFQVSAATINMIAEISALIERFVIRLEQADGLHLRKANR
                     IKTIRGTLAIEGNTLSESLITDIIAGKTVVAPIREIQEVKNAVKTYNAYPTLDPFSVK
                     DMLKAHGLMMEALADDAGHFRKREVGVFQGNVPIHVAPPAGKVPYLMDDLFKWLRNSK
                     DHLLIKSCVFHYEFEFIHPFSDGNGRIGRLWQSLILGKLNPVFEHLPVENMVFSNQQK
                     YYQAIQKSTQKADSGIFIDFMLEEILKTLKERKGEPLKKHYQSSTSSVPDQYQSQPES
                     QPESQPESLEQKIISILKAGEFSKAEISNKLGQKEISGRLNQVI"
     gene            complement(1053..4184)
                     /locus_tag="PL056_08610"
     CDS             complement(1053..4184)
                     /locus_tag="PL056_08610"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47117.1"
                     /translation="MKRLFVIFLICLPILLSAAMGPDVSQLDGKALKWEEGAWDYFTM
                     FKTLLRNDNRTVCTPSALTQCNLGDDQPGNPQADACADPSIGTTFTLIDSQIPFDARV
                     QAAYLIWISALPSEKVPGGDTDNTVTLSFENQNGSVTLSSEISATEKPMKATDPQNFS
                     FSAHESINPVNLTCTTDDECTSNAQLGLGWKCISGKCSTHNVVYTYRADVTEFFGTIH
                     EMSAAADIPNDGIGLLGKYTVSNMECTNDASYLATSGMVGGWALVFVYTSEQISPKKI
                     YFYDGFEAYRFEEGIISTSGFELPNEAVVRFTMMSAEGDPGLATSQNTENPFGPPPPA
                     EALYISGAQKFPIGEWLQLWNDCNQAKNTDSQNLPFNYTEVFNSISSTYGWADEVPFC
                     VGGLPGAVDPNILEYAMDVDTFILDAKDEVFAPHLIKGDQFLNFKIGANQDQIYTNLI
                     VVSIDTKAPKFDIPKNPDTPDGREKNSCTCSETPDFICSDRPFYFTIKVENWGENIAE
                     NVTVEDSLPRNVNYIAGSTEMLTTKDGVTGKWEKVEDASGGGFPFSSPRMVMDSMYYC
                     DKDTFECRDRVMIRFKVKPIDTIQKNETVDNTAIITDSSGIQYFSNSSIALRLKQGNC
                     PSITDCPEPPKELCGPSSGPECTKKEDCEEGYDCKDEKCVKDTGSISKDAVITFAEGK
                     NNPTADGSSIIIPNPSKNTVTSQFTLMAEGDGSKSFQLNGLNVSVFTSSTEVSVKNLR
                     LIYDENGDGEAGSKEKVLSTVESAKANVAAFSLLNESDRAFVTGKLHYFIIVADASFD
                     GEPSNNDNFNLEIEGSVAFLISDNGKPDVKGEQITFATFKFEPENGFIITKGKNDPQV
                     PSIDKMKGMVPVLQLRTKSMQGDDTLKSINFKPYLKSVKFGEGINGAVLYLDKNSDGK
                     IDSGDTALSEISSFTSITNGIFDNLNLKYSKAEEKHLLIALDLNLSTGQLAQTQVGKV
                     SVSPDREIVELPVTSKEFKYECAAGDPTCAEDEEESGCAVTTVDNGMSVYSLILAALL
                     ALSAIFGLNIIRK"
     gene            4406..5404
                     /gene="argF"
                     /locus_tag="PL056_08615"
     CDS             4406..5404
                     /gene="argF"
                     /locus_tag="PL056_08615"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019214858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HPM47118.1"
                     /translation="MAVNLKGRHFLTLKHFTPEEIRYLLDFSHNLKAKKRAGIKGDLL
                     EGKNIVLLFEKASTRTRCAFEVAALDEGGHITFLGSNDSQMGKKESIEDTAKVLGRFY
                     DGIEFRGFKQETVEALAKYAGVPVWNGLTDLYHPTQILADFMTVEEHVKKPINKVKFV
                     YCGDARNNMGNSLMIGAAKMGMHFVACAPKALFPDKKLIDEMEKIAKETGAKLEFIED
                     VDKAVINADVIYTDVWVSMGEEHLFEQRIKQLKKYQVNMAMIKKTKNPDVIFLHCLPS
                     FHDLNTKVGNEVFEKYGVKEMEASDEVFRSKHSKVFDEAENRMHTIKAVMCATIGK"
     gene            5405..5815
                     /locus_tag="PL056_08620"
     CDS             5405..5815
                     /locus_tag="PL056_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47119.1"
                     /translation="MKRQIIKSLIFFSFVFMIAGCFSSPEGRLKSIDFQKVQQAVDEE
                     DPQKSKELLDPQIEKVGKLCDSLKAARIENRHFDAVDQYRRYDFMTSLGGIAIVQENL
                     KNGTIVEISGKDKEEIKFICDKLVFDANQVLNLK"
     gene            complement(5820..6512)
                     /locus_tag="PL056_08625"
     CDS             complement(5820..6512)
                     /locus_tag="PL056_08625"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HPM47120.1"
                     /translation="MITRSEKKIIGGHRGSPKKARENTIGSFEAAIMDGADFIEFDVR
                     RAKDGNLVIHHDEMIKGIPVKKLKLSEINEISLQMGFEVPVLEDVLRHCVNRIMLDVE
                     IKEPDITGEAVKQILRYFSPDGFIVTSFYDETLLNLNTYHPKIKKGLLFDKNSGHDLE
                     KRIMSLKPDFLLPHYSSFNISVVELCIKFKLRAILWTVNNENDMIKFISDPLVAGIIS
                     DHPEIAKRIQKN"
     gene            complement(6509..7639)
                     /locus_tag="PL056_08630"
     CDS             complement(6509..7639)
                     /locus_tag="PL056_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HPM47121.1"
                     /translation="MNYGSNYKSCVWTDFMLLENFMKDVFIKLGTPVQDSEIIADVLI
                     TADKKGIDSHGIGRFKLIYIDRIDQGILSPKTLINVVKETETTAVLDGNNGMGHVISK
                     KAMNMAIEKARKFGTGMVAVRNSSHYGIAGYYVDMCSDNDMIGITGTNARPSIAPTFG
                     VENMLGTNPLTIGFPTDEKFHFNLDCATSVTQRGKIEAYERAEKILPEGWVVGSDGKT
                     MTDAKTVLEALTKGTAALTPLGGLGEDTAGYKGYGYATAVEVLSAALSNAKFMKDLNG
                     LDESGKKIPYPLGHFFIAIDIKSFIDPAIFRSIAGTMMRNLRNSKKAEGAERIYTAGE
                     KEYIAWQYRKEHGCPVPPVLRDQLSALNMRFGLEYKFPWENK"
BASE COUNT         2121 a         1580 c         1619 g         2330 t
ORIGIN      
        1 aatgacttgg ttcagtcttc ctgatatttc tttctgccct aatttattag atatttcagc
       61 ttttgagaac tctcctgctt tcagaattga tataattttc tgctcaagtg actctggctg
      121 tgactctggc tgtgactctg gctgtgactg gtactggtct ggtactgaac tggtactgga
      181 ctggtaatgt ttcttcagcg gttctccttt tctttctttc agcgttttca atatttcttc
      241 aagcataaaa tcgatgaaga tgccggagtc tgctttctga gtgctttttt gtattgcctg
      301 ataatatttc tgctggtttg agaagaccat gttttcgact gggaggtgtt caaagacggg
      361 gttgagtttt ccgagtatga gtgactgcca gagtcgtcct attcttccgt taccgtcaga
      421 aaaagggtga atgaattcaa actcatagtg aaagacacaa ctttttatga gcaggtgatc
      481 ttttgaattt ctcagccatt tgaagaggtc atccataaga taggggactt tgccggcggg
      541 aggtgcgaca tggattggca cattcccctg aaaaacgcct acttcccttt ttctgaaatg
      601 accggcatca tcagcaagtg cttccatcat gagcccatga gctttaagca tatctttaac
      661 tgaaaacgga tcaagtgtcg gataggcgtt ataagttttg acggcatttt ttacttcctg
      721 aatttctcgt ataggtgcga caacggtttt tcctgcaatg atgtcagtta tcaaactttc
      781 agaaagagtg ttcccttcga tcgcaagagt tccgcgaatc gtctttatcc ggtttgcttt
      841 ccttaagtga agcccgtccg cctgctcaag acggattaca aacctttcaa taagagcaga
      901 aatctccgct atcatgttta tcgtcgctgc actcacttga aacggtggac ggtaactcat
      961 attacctcaa attcaaaagt tgcataaata tactgtttta atggagatta atcaatttaa
     1021 aactgaaggt gttggagttg gggactggtt atttatttcc tgataatatt aagtccaaat
     1081 atagctgaaa gggcaagaag cgctgcaagt attaatgaat aaacagacat tccgttgtca
     1141 actgttgtaa cggcacatcc agattcttct tcatcttctg cacatgtcgg gtcgcctgcc
     1201 gcgcattcat atttgaattc ctttgaagtt acagggagtt caacaatttc gcgatccggt
     1261 gatacgctca ctttgccgac ttgcgtctga gcaagctgac ctgttgagag attgagatca
     1321 agtgcgataa gaagatgttt ttcttcggct tttgaatatt taaggttcaa attgtcaaaa
     1381 attccgttcg taatgcttgt aaatgatgat atttcagaaa gcgcagtgtc accgctgtcg
     1441 atctttccat cgctgttctt gtcaagataa agcacagcgc cgtttattcc ttcaccgaac
     1501 ttgaccgatt taagataagg tttgaaattt atcgacttaa gcgtgtcgtc accctgcatg
     1561 gatttggttc tgagctgaag aacaggcacc attcctttca ttttgtcaat tgacggaacc
     1621 tgcggatcgt tcttcccttt agtgataata aatccgtttt ccggctcaaa tttgaaagtt
     1681 gcaaaagtaa tttgctcacc tttaacatca ggttttccgt tatcggaaat gaggaaagcg
     1741 acagaacctt ctatttcaag gttgaaattg tcgttatttg acggttcacc atcaaacgaa
     1801 gcgtctgcaa ctatgataaa atagtgaagt ttgcccgtta cgaatgcccg gtctgattca
     1861 tttaaaagag aaaatgccgc aacatttgcc tttgcggatt caacggtgga aagaactttt
     1921 tcttttgatc cggcttctcc gtctccattc tcatcataaa taagccggag gtttttaacc
     1981 gaaacttctg tagagcttgt gaaaactgat acattcagcc cgttgagctg gaacgattta
     2041 cttccgtcac cttcagccat taatgtgaac tgtgatgtaa cggtgttctt tgaagggtta
     2101 ggaattatta ttgatgaacc gtcagctgtc gggttgttct tcccttcagc aaatgttata
     2161 acggcatctt tcgatattga tccggtatct ttgacacatt tttcatcttt acagtcatat
     2221 ccttcttcgc agtcctcttt ttttgtgcat tcagggccgc ttgaaggtcc gcaaagctct
     2281 ttcgggggtt caggacagtc ggtaattgac ggacagttcc cctgtttaag tcttaaagca
     2341 atggatgagt ttgaaaaata ctggatcccg gatgagtcgg ttattatcgc ggtgttgtca
     2401 acagtttcat ttttctggat agtgtcgatc ggtttgactt tgaaacggat catcaccctg
     2461 tcacggcatt cgaatgtgtc tttgtcgcag taatacattg aatccattac cattctcggt
     2521 gatgaaaaag ggaatccgcc accggatgca tcttcaactt tttcccattt tcctgtgacg
     2581 ccgtctttag tcgtaagcat ttcggtcgaa ccggcgatat aattcacatt tcttggaaga
     2641 gaatcttcaa cagtcacatt ttctgcaatg ttctcgcccc agttctcaac tttgatcgtg
     2701 aaatagaaag gtctgtctga acatatgaaa tcaggtgtct cggaacaggt gcagctgttt
     2761 ttctcacggc cgtcaggtgt gtcaggattc ttaggaatat caaatttggg ggcctttgta
     2821 tcgatagaga caactatcag attggtgtag atctggtcct gattggcacc gatcttgaaa
     2881 ttgaggaact gatctccttt aataagatgg ggagcaaaaa cttcatcttt cgcatcaaga
     2941 atgaatgtgt cgacatccat cgcatattca agaatattgg ggtcaactgc accgggaagt
     3001 ccgccgacgc agaaaggaac ttcgtcagcc cagccgtaag ttgaagagat cgaattgaaa
     3061 acttccgtat aattaaacgg gagattttgt gaatctgtgt tttttgcctg attacagtca
     3121 ttccagagct ggagccattc accgataggg aatttctgtg cacctgaaat gtagagtgcc
     3181 tctgcaggtg gaggcggacc aaaaggattt tcagtatttt gagaagttgc gagtcccgga
     3241 tctccttcag cagacatcat tgtaaatctg acaactgctt cgttcgggag ttcgaaaccg
     3301 cttgtagata taatgccttc ttcaaatcta tatgcttcaa atccgtcata aaaatagatc
     3361 tttttggggc tgatctgttc tgaagtgtaa acaaaaacga gagcccatcc gcccaccata
     3421 ccgcttgtgg cgagatatga agcatcgttg gtacattcca tgtttgaaac agtatatttt
     3481 ccaagaagtc cgataccgtc attcggaatg tcagccgcgg cactcatttc gtgaattgtt
     3541 ccgaaaaact cagtaacatc ggcacgatag gtgtaaacga cattatgggt ggaacacttt
     3601 ccgctgatac acttccaacc taatccgagc tgagcgttgg aggtacattc atcatcagtt
     3661 gtacaggtaa gattaacagg gtttatagat tcatgggcac tgaagctgaa gttttgcgga
     3721 tctgttgcct tcatcggttt ttctgtggct gatatttcag aactcaaagt tacagacccg
     3781 ttctgatttt caaaagacag agtgaccgtg ttgtcggtat caccgcccgg aactttttca
     3841 gatggaagag cacttatcca tatcaggtaa gccgcctgaa cccttgcatc aaaaggtatc
     3901 tgagaatcga tgagcgtgaa ggttgtgcct attgaagggt cggcacatgc atctgcctgc
     3961 gggttccccg gctggtcatc tccgaggttg cactgggtaa gtgcacttgg agtgcagaca
     4021 gttctgttgt cgtttctaag aagtgttttg aacatggtga aatagtccca tgcaccttcc
     4081 tcccatttga gcgcttttcc gtcaagctgg gaaacatcgg gtcccattgc cgcggaaagc
     4141 aggatcggca ggcaaattaa aaaaatcaca aacagtcttt tcattttaac tcctcgcaaa
     4201 aatattttac aaaaacacgc aacacaaagc gataaaccac attttcaaag atcttttcga
     4261 ccatttcggc agaaaaggac acaatttaat attataatag tacggatttg taaagaagaa
     4321 cttttcaaaa tacatttata ttgattttta atatacccaa tttataatcg gccgttgttt
     4381 gtttaacttt tttttggaga aaaaaatggc tgtaaatctt aaaggaaggc acttcttgac
     4441 acttaaacat ttcacacctg aagagatccg ctatcttctt gacttttccc ataatcttaa
     4501 agcaaagaaa agggcaggga tcaaaggaga tcttcttgaa ggaaagaaca tagtccttct
     4561 ttttgaaaaa gcttcgacaa gaacccgctg cgcttttgaa gttgcggctc tcgatgaagg
     4621 cggacacatc acatttcttg gctcaaacga ttcacagatg ggcaaaaagg aatcgatcga
     4681 agacactgca aaagttctcg gcagattcta tgacggaata gaattcagag gtttcaagca
     4741 ggaaaccgtt gaagcacttg caaaatatgc aggcgttccg gtctggaacg gacttactga
     4801 tctctatcat ccgacacaga tacttgctga tttcatgaca gttgaagagc atgtgaaaaa
     4861 accgataaac aaagtaaaat tcgtatattg cggagacgca aggaacaata tgggcaattc
     4921 actcatgatc ggtgcggcaa aaatgggaat gcattttgta gcatgtgctc ccaaagccct
     4981 tttcccggat aagaaactca ttgacgaaat ggaaaagatc gcaaaagaga ctggtgcaaa
     5041 acttgagttc atcgaggatg ttgacaaagc ggtgatcaat gcagatgtaa tttataccga
     5101 tgtgtgggtt tcaatgggtg aagagcacct tttcgaacag aggatcaaac agctcaaaaa
     5161 atatcaggta aatatggcga tgatcaaaaa gaccaaaaat cctgatgtga tcttccttca
     5221 ctgcctgccg agtttccacg acctcaacac aaaagtcgga aatgaagtgt ttgaaaaata
     5281 cggtgtgaaa gagatggaag caagcgatga agttttcaga agcaagcact caaaagtgtt
     5341 tgacgaagca gagaacagaa tgcacacgat caaagctgta atgtgtgcaa cgatcggcaa
     5401 ataaatgaaa agacagataa ttaagtcttt aatattcttc agctttgttt ttatgatcgc
     5461 tggttgtttt tcatcgcctg aaggtcggct taaaagcata gattttcaga aagtacagca
     5521 ggcagttgat gaagaagatc cacaaaaatc gaaggaactt cttgatcccc agatagaaaa
     5581 agtggggaaa ctttgtgatt ctctcaaagc ggcaagaatc gaaaaccgcc attttgatgc
     5641 agttgaccag tacagaaggt atgacttcat gacatctctt ggcggaattg cgatcgttca
     5701 ggaaaatctt aaaaacggaa ctatcgtcga aatttcaggc aaagacaaag aagagataaa
     5761 gttcatctgt gacaagctcg tttttgacgc aaaccaggtc ctcaacttga aatagttgat
     5821 caattcttct gaattctttt agcaatttcc ggatgatctg aaataatccc tgctacaagc
     5881 ggatcgctga tgaactttat catgtcattt tcgttgttta cagtccagag aattgcacgg
     5941 agtttgaatt taatgcataa ctcgacaact gatatattaa atgaagagta gtgggggagg
     6001 agaaaatcgg gtttaagaga cataatccgt ttttcaagat cgtggccgga attcttgtca
     6061 aaaaggagcc ccttttttat tttaggatgg taagtgttca gatttaatag tgtttcgtca
     6121 taaaaagaag tgactataaa tccgtcaggt gaaaagtacc gcagtatctg cttaacagct
     6181 tcccctgtaa tatcgggctc ttttatttcg acatcgagca ttattctgtt aacacagtgt
     6241 ctcaaaacat cttcaaggac aggcacttca aatcccattt gtaaagaaat ctcattaatt
     6301 tcagacaatt tcagtttctt aacaggaatt cccttgatca tttcatcatg atggatgaca
     6361 agattgccgt ctttggctct tcttacatca aattcaatga agtctgctcc gtccatgatg
     6421 gcggcttcaa agcttccaat tgtattttct cttgcttttt tagggcttcc gcgatgacca
     6481 ccgatgattt ttttttcaga tcttgtgatc atttgttttc ccacggaaat ttgtattcta
     6541 gaccgaatct catgttcagt gccgaaagct ggtctcttaa cacaggcgga actggacagc
     6601 catgctcttt tctatactgc catgcaatat attccttttc tcctgcagtg taaatccttt
     6661 cagcaccttc agctttcttt gaatttctca aattccgcat cattgttcct gcaattgatc
     6721 tgaaaatagc gggatcaatg aaacttttta tgtctattgc gatgaaaaag tgtccaagag
     6781 gataggggat cttttttcct gattcatcta atccgttaag atctttcatg aatttggcat
     6841 ttgaaagagc agcggaaaga acttcaactg ccgttgcata accataaccc ttgtagccgg
     6901 cggtatcttc accaagaccc ccgagcggag tgagtgccgc tgtccctttt gtcagcgctt
     6961 caagaactgt tttcgcatcg gtcatagttt ttccgtcact tccaacaacc catccttccg
     7021 gaagaatttt ctcggcccgt tcgtatgcct cgatctttcc tctctgagtt actgaagttg
     7081 cacagtcaag gttgaaatga aacttttcat cagtcgggaa accaatcgtc agcggatttg
     7141 ttccgagcat gttctcaaca ccgaaagtgg gggcgataga tggtcttgca ttagtccctg
     7201 tgatgccgat catatcatta tctgaacaca tatcaacata gtagccggca attccgtagt
     7261 ggcttgaatt tcttacagcg accattcctg ttccgaattt cctggctttt tctatcgcca
     7321 tgttcattgc ttttttagaa atgacatgac ccataccgtt attgccgtca agcactgccg
     7381 ttgtttcagt ctcttttaca acatttatca aagtctttgg agaaagtatc ccctggtcga
     7441 tccggtcaat atatatcagt ttgaatcttc ctatgccatg tgaatcgatc ccttttttgt
     7501 cggctgtaat taaaacatcg gcaattattt cagaatcttg aacaggcgtt ccgagtttta
     7561 tgaagacatc tttcataaaa ttttcaagaa gcataaaatc agtccagaca cagctcttat
     7621 agttgctgcc gtagttcatg acattcctcc
//
LOCUS       DAOOYB010000135         7487 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_11126_length_7487_cov_12.316066, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000135 DAOOYB010000000
VERSION     DAOOYB010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7487
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11126_length_7487_cov_12.316066"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="PL056_08635"
     CDS             complement(<1..688)
                     /locus_tag="PL056_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47122.1"
                     /translation="MFFLKHFKLFGVFFLVAAFLVSGCEAPKTRIIAQKDDDGAISDI
                     DEIDDIENSDAEENDTEKKDDVVHDDDSVIKTCGNSVVDTGELCDGNVKNCTEIDPVK
                     YIAGKAKCGEDCLGWDTETCEENPYECGNGIVEGNEICDRGVKNCIEIDPLKYESGKA
                     KCLENCIGYDTATCVDLEIPDEDTGDTGNTGDTGNTGDTGNTGDTGNTGDTGNTGDTG
                     NTGDTGNTGDT"
     gene            complement(698..2104)
                     /locus_tag="PL056_08640"
     CDS             complement(698..2104)
                     /locus_tag="PL056_08640"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HPM47123.1"
                     /translation="MYLLIFRGGKHLFSFYVNSAKKRIVVGGDEGDILFPPEMRGSEM
                     VLRKEFNFEKKEYSWCLFPVSGKFKLNGKDVAGGEIFEGTDVFSSGDFSFSFSPISNL
                     STVVPEENSKKFGNTLIMEDEPAEYKNVVMTYGGKGKTFVFRPGRDIKIGRKNTDVSI
                     SFDEVSSEHVLFEMKETGLHFWNKGKNGTWVNGVKIESGVLEEGKYHFSIAGRHDVLI
                     SVNKEKTSESYYIGTLAPQFEQIENWLNQPALFANHPIILLTGESGTGKEVFSEFIHK
                     TSGRKGQFITYNAASIPETLVESELFGTVKGSFTGAEERTGAFIAADGGTLFLDEIAE
                     MPMNLQSKLLRVLEDWMVRRVGESGTGRKVDVTLILATNKDLEKAVKAQTFRKDLYYR
                     LATLHLNIPPLRERKDDIIPLCRHIHSALKGKDLKIEKAAEKKLLEHDWPGNVRELKS
                     VITRFAYSSGDTFKVKDF"
     gene            complement(2186..3889)
                     /locus_tag="PL056_08645"
     CDS             complement(2186..3889)
                     /locus_tag="PL056_08645"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013326323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPM47124.1"
                     /translation="MTEKKQKTEKTENSEMMTQSTFESFGFKKEILDGIRDAGFKVPS
                     PIQEQVMPLILKGKDVIAQSHTGTGKTAAFGLPIMNNMQKNIGIELLVITPTRELAAQ
                     ISDEIFRLGKYTGIRTGTILGGHSYSRQLQILKNGVQVLAATPGRLLDMLKSGKIAIK
                     EPITIVLDEADEMLDMGFLEDITEIFKFIPEKRQTLLFSATMPEPIRRLAQKILHEPV
                     FIRAMTAGESTNIDIGQSYYVIEEHERELAVVRLMEDQDPEKAIVFCRTKLEVDKLST
                     FLVARGYNAKGLHGDMEQQQRNQVMTSFRKSMIDILVATDVAARGLDVADVTHVFNYH
                     MPFDAKSYIHRVGRTGRAGHKGMAITLVTPSEFRTIQRIEKSVGSTIELKVIPTLKNI
                     KEERIKRLAAEMMEHTPDKEGLALVEKLENEMDIRSIAVVLASMLLGEDEETGPEQIG
                     FTESRLRQLIQRDKEGFNRGRSGGRDNRSHRDRDRHSHHSSDDRKRKFRSDEKPYEPD
                     KPPKDKKPFDKDKKPFDKDKKPFDKDKKPFDRNKPFKDKKPFDKNKKPFDKNKKKKID
                     K"
     assembly_gap    4044..4143
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4246..5337)
                     /locus_tag="PL056_08650"
     CDS             complement(4246..5337)
                     /locus_tag="PL056_08650"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HPM47125.1"
                     /translation="MNNEIIEKFKKCVVQIATPYSSGTGFILFSEGVIVTNYHVVKNC
                     EEVVANGREFKKTKVKVLFIDPHFDVAFVESPLPKSDGGPHLSEGPVLEGESVLAIGH
                     PLGLKYTSTGGIVSKTERDYNGIKYFQIDAAINPGNSGGPLINSKGDIIGVNTFIIRD
                     AESLAFSLPYFYVKECLESYGKFAGKSAVRCRSCRKTQLKEELGSGFCANCGFRFDKD
                     DVATRPFIPEGIIAKIENIISRSGFDAELSRCGAMLWEISNELVFVKITYVQATKFII
                     FDAPVSYVPEEKISEFFEFALKENFDLKNVIFSLKNNELYLSVITFEDDFHEETGFNK
                     LKELIEFATAYRKNLEFRYGAKPIHDIVE"
     gene            complement(5334..6653)
                     /locus_tag="PL056_08655"
     CDS             complement(5334..6653)
                     /locus_tag="PL056_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47126.1"
                     /translation="MGIFDFFTGKSSAGNVNSDNYGRFLECNKPLYFSNIFKEATDLF
                     KAEKYSESFMKFLDYISNPPSQNISYEKKGNDIEFTIIHGSAKITGVFNSEGVYATSE
                     IGQYSGVATALFRDLLTKNFSMNYNGFCIQDSRVYLKHRSPLKESSPMKLYYALKEMG
                     LKADIHSQSLFDDFANLKESSARAKIEVPVSEKMIKIKYLKQWIKDTFELTSSMSPEK
                     DGNLMSHYLLALCYRIDFLLVPKGKIWKTNDMAVAIYSDKVLSQSEKIRKMLSYLKDI
                     LDMSDFEIGESFFRTVDTFGLVNGSAHNDLVQFYLDQFNSAKYYVDLQQEKNTMAVYE
                     QALGYSLYFMGIYPAVKKLLLLYYRIFYPAFFEEFDIKSDFFNAETKKFNKRAIENEI
                     SDIISKERKGYPSLTFLSHNLRYETPSRFTFTFLNEITYLNFFQPVK"
     gene            complement(6725..6907)
                     /locus_tag="PL056_08660"
     CDS             complement(6725..6907)
                     /locus_tag="PL056_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006627018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HPM47127.1"
                     /translation="MKKLTLSIDRKFLGVCGGIGEYLEVDPAVIRILWVIATFVSFGM
                     GLLVYFICFVIMSSPR"
     gene            complement(6970..7272)
                     /locus_tag="PL056_08665"
     CDS             complement(6970..7272)
                     /locus_tag="PL056_08665"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPM47128.1"
                     /translation="MAKKTKLVATTKTDFIKVLKENVGATLTNDQAKQVYDVFTTILK
                     DTVIAERKLNLNGLGTFKVSNRKARTGINPKTREAIKIKASKSVGFKPNPTFKKGL"
BASE COUNT         1925 a         1717 c         1385 g         2360 t
ORIGIN      
        1 ctgtatctcc tgtgttacct gtatctcctg tgttacccgt gtcacctgtg ttgccggtat
       61 cgcctgtatt gccagtatcg cctgtgttgc cagtatcgcc tgtgttgccg gtatctcctg
      121 tgttgccagt atcgcctgtg tcttcgtcgg ggatctccag atctacgcat gttgcagtgt
      181 cataacctat acaattttca agacattttg ctttgcctga ctcatatttc aaaggatcta
      241 tctcaatgca attctttact ccgcggtcac agatctcatt tccttcaact atgccgttac
      301 cgcattcata cgggttttct tcacaggttt cagtatccca gcccaggcag tcttcaccac
      361 attttgcttt tcctgcaata tatttcacag gatctatttc tgtgcagttc tttacatttc
      421 cgtcacaaag ttccccggta tctacaacgc tgttgccgca tgtctttatc actgaatcgt
      481 catcatgcac aacatcatcc tttttttctg tatcattttc ttcagcatca ctattttcaa
      541 tgtcatcaat ttcatcaatg tctgatatcg ccccgtcatc atccttctgc gctatgatcc
      601 ttgttttagg tgcttcacaa cccgaaacaa gaaaagccgc aacaagaaaa aaaacaccaa
      661 acagtttaaa atgtttgaga aaaaacatcc cgccccccta aaaatctttc actttgaaag
      721 tatcacctga agaatatgca aatcttgtta taacactttt caattctctc acatttcccg
      781 gccagtcatg ttcaagaagc ttcttttctg ccgctttttc aatcttcagg tcttttcctt
      841 taagtgctga atggatatgt ctgcaaagag gaataatatc atctttcctt tcccgaagcg
      901 gtggaatgtt caaatgaagc gttgcgagac ggtaataaag atcttttctg aatgtctgtg
      961 cctttacagc tttttccaga tccttatttg tggcaagaat cagtgtcaca tcaacttttc
     1021 ttcccgtacc gctttcacca actcttctca ccatccagtc ttcaagaact ctgagcagtt
     1081 tagattgaag gttcatcggc atctctgcta tctcatcaag gaaaagtgtt ccgccgtcag
     1141 ccgcaatgaa agctcctgtc ctttcctctg cccccgtaaa actccccttc acagtcccga
     1201 aaagttcgct ttcgacaagt gtctctggga tacttgccgc gttatatgtg ataaactgcc
     1261 cttttctgcc gcttgtctta tggatgaatt cggaaaaaac ctctttgccc gttccgcttt
     1321 cacctgtcag aagtattatc gggtgatttg caaaaagagc cggctgatta agccagttct
     1381 caatctgttc aaactgtgga gcaagagttc ctatgtagta tgattcactg gttttttcct
     1441 tgttcaccga tataagaaca tcgtgtcttc cggcaattga gaaatggtat tttccctctt
     1501 caagcacacc gctttcaatt ttcacaccgt tgacccatgt tccgttcttt cccttattcc
     1561 agaaatggag tcccgtctct ttcatttcaa aaaggacatg ttcgcttgaa acttcgtcaa
     1621 aacttattga aacatctgtg ttcttcctgc cgatctttat gtcacgcccc ggtctgaaaa
     1681 caaatgtctt cccctttccg ccatatgtca tcacaacatt tttatattcc gccggctcat
     1741 cttccatgat aagggtgttg ccgaattttt ttgaattttc ttcaggaacg acggttgaaa
     1801 gattggatat tggagaaaag gaaaatgaaa aatcacctga tgaaaacaca tcggtccctt
     1861 caaaaatttc accacctgca acatctttcc cgtttagctt gaacttcccc gacacaggaa
     1921 aaagacacca tgaatactct tttttctcaa aattgaattc cttgcgaagg accatttcgg
     1981 aacccctcat ctcaggagga aaaaggatat caccctcgtc accgcctaca actatccgtt
     2041 tttttgcact gttcacatag aatgagaaaa ggtgttttcc acctctgaaa attagaagat
     2101 acatctttta ctccggcaaa ttcaaatatt acgcgatatt ttacaaactt agatgataaa
     2161 aggcaaattt agggcgatca atgactcatt tatctatttt cttcttcttg tttttgtcaa
     2221 acggcttttt gttcttatca aaaggttttt tgtctttaaa tggcttgttc ctgtcaaagg
     2281 gttttttatc cttatcgaag ggttttttat ctttatcaaa aggtttttta tctttatcaa
     2341 agggtttttt atcttttggc ggcttatccg gttcgtaagg tttctcatct gatctgaact
     2401 ttcttttccg gtcatctgag ctgtgatgac tgtgacggtc cctgtcacgg tgacttctgt
     2461 tgtctcttcc gccggacctt cctctgttga aaccttcctt gtctctctgt atgagctgtc
     2521 tgagccgtga ttcagtaaaa ccgatctgtt ccggacctgt ttcctcatct tcaccaagaa
     2581 gcattgaagc aaggaccaca gctatcgatc ttatgtccat ttcattctca agcttttcaa
     2641 caagtgcaag tccttccttg tctggagtgt gctccatcat ttcagccgca agccgtttga
     2701 tcctctcttc ttttatgttt ttcagtgtcg gaatgacttt tagctcgatc gttgagccga
     2761 cacttttttc aattcgctga attgtcctga attcggatgg agttacaaga gttatagcca
     2821 ttcccttatg ccctgcacga cctgttcttc cgactctgtg gatataactt ttagcatcaa
     2881 aaggcatatg gtagttgaaa acatgggtca catcggcgac atcgaggccc ctggccgcca
     2941 catcggtagc aacaagaatg tctatcatgc tttttctgaa acttgtcatg acctgatttc
     3001 tctgctgctg ttccatatca ccgtgaagtc cttttgcgtt gtatccgcgg gcgacaagga
     3061 aagtggaaag cttatcaact tcgagcttcg ttctgcagaa aactatcgcc ttttcaggat
     3121 cctggtcttc catgagccgt acaacagcaa gttctctttc atgttcttct atcacataat
     3181 atgactgacc gatgtcaata ttcgtagatt ctcctgccgt cattgccctg atgaaaacgg
     3241 gttcatgcag tattttctgg gcaagccgtc ttatcggctc gggcattgtc gctgaaaaaa
     3301 gaagtgtctg ccttttttcc gggataaatt taaatatttc ggtaatatct tcaagaaatc
     3361 ccatatcaag catctcgtcc gcttcatcaa gaactatcgt tatcggctcc ttgatcgcga
     3421 tcttgccgga tttcagcatg tcaagaagcc gtcccggtgt tgcggcaaga acctgaacac
     3481 cgtttttaag tatctggagc tgtcttgaat atgaatggcc tccgaggatc gttccggttc
     3541 ttatgccggt atattttcca aggcggaata tctcgtcact gatctgtgcg gcaagttctc
     3601 ttgtcggagt tattacaagc agttcaattc cgatgttctt ttgcatgttg ttcatgatcg
     3661 gcagtccgaa agcggcagtt tttccagtcc ctgtgtgaga ctgagctata acatcttttc
     3721 cctttaagat cagaggcata acctgttcct gaatcggact cggcacctta aaaccggcat
     3781 ctcttatacc gtcaagtatc tcctttttga acccgaaaga ttcaaaagta gactgtgtca
     3841 tcatttcact gttttctgtt ttttctgttt tctgtttctt ttctgtcatc ttatttcctt
     3901 gattaatgtc tgtgcggtca atcaaactcc gcgaaaaaat taaagaatgg ctggttgcag
     3961 ccgcaccgct aaagtactta tgaatcagga tttgtcaacc cttttccaac cttacataaa
     4021 agacaaactc gccccggtcc ttcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnncggttaa atttaaaata gcgggctgaa agtccgcgtt atcttagccc acgcccaacg
     4201 ggctggggca acagacggtt agctggggaa atttatttcc ttttgttatt ccacgatatc
     4261 atggattggt tttgcaccgt atctgaattc gagatttttc ctgtaggcgg ttgcaaactc
     4321 aatgagctct ttcaacttat tgaaccctgt ctcttcgtgg aaatcgtctt caaatgtaat
     4381 gactgaaaga tacagttcat tatttttcaa gctgaatatc acatttttaa gatcaaaatt
     4441 ctctttgagg gcaaactcga aaaattcact gatcttttct tccgggacat acgaaaccgg
     4501 agcatcaaaa attatgaact ttgtcgcctg cacataagtt attttcacaa acaccagctc
     4561 atttgatatt tcccagagca tcgctccgca ccttgaaagt tcggcatcaa accctgagcg
     4621 agagataatg ttttcgatct ttgcgatgat cccttccgga atgaacggtc ttgtagcgac
     4681 atcatcctta tcaaatctga atccacagtt ggcacagaaa ccgcttccga gttcttcttt
     4741 taactgggtt ttccggcatg acctgcatct tacggcactt ttcccggcaa attttccgta
     4801 cgattcaaga cactctttca cataaaaata aggaagcgaa aatgcaagac tttcagcatc
     4861 tctgattatg aaagtgttga caccgattat gtcccctttt gagttgatga gcggaccacc
     4921 cgaatttccc gggtttatcg ccgcatctat ctgaaaatat ttgattccgt tgtaatccct
     4981 ttcagttttt gaaactatcc cgccggtaga agtgtatttc agtccgagcg gatgaccgat
     5041 ggcaagaacg ctttcacctt caagcaccgg accttcggaa aggtgcggtc ccccatcaga
     5101 tttcggcagt ggcgactcaa cgaaagcgac atcgaagtgc ggatcgatga aaagcacttt
     5161 cactttcgtc tttttgaatt cccgtccgtt tgcaaccact tcttcacagt tcttcacgac
     5221 atgatagttg gtcactatca ccccttcaga aaaaaggatg aaccctgtac cagaactgta
     5281 gggtgtcgcg atctgcacga cacatttttt gaatttttca attatctcgt tgttcatttt
     5341 accggctgaa aaaagtttaa atatgtaatt tcgttcaaaa atgtaaatgt gaaacggctc
     5401 ggggtctcat atctgagatt gtgtgaaagg aaagtaagcg acggatatcc ctttctctct
     5461 tttgaaataa tgtcagatat ttcgttctcg atcgcccttt tgttgaattt ctttgtttcg
     5521 gcgttaaaga aatcgctttt tatgtcaaac tcttcaaaaa aagcggggta gaaaatacgg
     5581 taataaagaa gaagaagctt tttaacagca gggtatatcc ccatgaaata gagagagtaa
     5641 cccagcgcct gctcataaac cgccattgtg ttcttttcct gctgaagatc gacataatat
     5701 tttgcactgt tgaactgatc aagatagaac tgtacaagat cgttatgagc cgaaccgttc
     5761 acaagaccga aagtatcaac cgtcctgaag aaagattccc cgatctcaaa atcactcatg
     5821 tcaagaatgt ccttgagata tgaaagcatt tttcttatct tttcgctctg tgaaagcact
     5881 ttgtcgctgt atattgcaac agccatgtca tttgtcttcc agatctttcc cttcggaaca
     5941 agaagaaagt cgatccggta acaaagtgca agaagataat gactcatcag gttgccgtcc
     6001 ttttccggag acattgatga agttaactcg aatgtgtctt ttatccactg cttcaagtat
     6061 ttgatcttta tcatcttttc tgaaacagga acttcaattt tcgcccttgc ggaactttcc
     6121 ttcagatttg caaaatcatc aaaaagcgac tgcgagtgga tatccgcttt aagccccatc
     6181 tctttcaggg cataataaag tttcatggga cttgattctt tcagaggaga acggtgtttg
     6241 agataaactc ttgaatcctg aatacagaat ccgttgtagt tcatcgaaaa attcttggtc
     6301 agaagatctc tgaaaagcgc tgtcgcaact ccgctgtact gacctatctc ggaagttgca
     6361 taaacccctt cactgttaaa cacaccggtt attttcgcac tcccgtgaat gatcgtgaac
     6421 tcaatatcat ttcccttttt ttcgtaactg atattctgcg aaggcggatt cgagatataa
     6481 tcaagaaact tcatgaagct ttcgctgtat ttttccgcct tgaaaagatc tgtcgcctct
     6541 ttgaaaatat tggaaaaata aagtggcttg ttgcattcaa gaaacctgcc gtaattatct
     6601 gaattgacat ttccggcact gctctttcct gtaaaaaaat caaatatccc catgaccgcc
     6661 ctttccaaaa aattgcttca acaaatatac ttattctgtt tcataataca aataaagaat
     6721 tagcttacct cggcgaagac atgatgacaa agcagataaa ataaaccagc agccccatcc
     6781 cgaaagaaac aaaagtcgcg atcacccata aaatccttat aactgcagga tcgacctcaa
     6841 gatattcgcc tattccgcca cacacaccga gaaatttcct gtcaatcgaa agagtgagct
     6901 ttttcatgat taacctccaa aaaaaaccct tcccgaagga agggtcataa acttcatgag
     6961 aaatttaaat cagagacctt tcttgaaagt cgggttcggt ttgaaaccta ctgactttga
     7021 agctttgatc ttgatagctt cgcgggtctt aggattgatg cctgtgcgtg cctttctgtt
     7081 gctgaccttg aaagttccca gaccgttaag gttgagtttc ctttcagcaa ttacagtgtc
     7141 tttaaggatc gttgtgaaaa catcataaac ctgttttgct tgatcattgg taagggtagc
     7201 gccgacattc tccttgagaa ctttgatgaa atctgtcttt gttgttgcaa caagtttggt
     7261 cttcttagcc atgaatctct ccattaaaaa agaataataa tagggtccgg gttcggtgta
     7321 tgatagatta aatcaggaga aaatgaaagt tttttttacg gcatgctgaa atttgcttat
     7381 gattgatgat cttccgaccg tgaacagctt gttgctgcaa agaaagatga atgggaaaaa
     7441 atggtggtcc cgacaggagt cgaacctgtt gcctatcact taggagg
//
LOCUS       DAOOYB010000136         7431 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_11249_length_7431_cov_9.428959, whole genome shotgun sequence.
ACCESSION   DAOOYB010000136 DAOOYB010000000
VERSION     DAOOYB010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7431
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11249_length_7431_cov_9.428959"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2339)
                     /gene="lon"
                     /locus_tag="PL056_08670"
     CDS             complement(<1..2339)
                     /gene="lon"
                     /locus_tag="PL056_08670"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013011111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HPM47129.1"
                     /translation="MNNGNGTTNIIPPSLPVLPLKDSVLFPYMIMNVNVQRGFSSSAV
                     NRAITAEGYLIVTAQKDPSIDQIKDETVYSTGTVATVIRMQKNFDGNMMLYIQGYKKA
                     SIRNIRYDEQGGYYTADVNVIDDEPDQNNVMSQALMTEIKRQIKNIADQGEIMLPPEA
                     LAAIQGVKNPGRLAEIIMSHLSKSVELSQRVLEENSVMKKLQIVHESLEKELSLIEIQ
                     SDIRNKAKERLGKAQKEYFLKEQMKQIQKELGNEDPHLSEIQEYEKKLDSIELPEKVR
                     TEAKKQLERLKTMNPAASETVVVKTWLDTILELPWMEETKDNKNIAKAGKILEEDHFG
                     LVDVKERILEFLAVRQIRGNSKSPILCFIGPPGVGKTSLGKSIARALDRNFYRLSLGG
                     LHDEAEIRGHRRTYVGAMPGKIIEGIKTSGSSNPVFMLDEIDKVGKDFRGDPSSALLE
                     VLDPEQNFSFLDNYIGIPFDLSKVFFIATANWEETIPAPLKDRMEIIRLSGYTDPERL
                     EIAKKYLIPRQLENCGLKQKELKFTNEALLFISRRYTRESGVRNLERNIGSICRKVTT
                     LKVQKKEFPKTITPKLVEKYLKLPPYSDTELDYDARIGVVTGLAWTQYGGSTLKIEAN
                     WMKGKGGLILTGQLGDVMKESARIALSYIQSNAETFGLGLDFTTEDKDIHIHFPAGAI
                     PKDGPSAGITITTALLSLFLKKEIDPFTGMTGEISLTGEVLPIGGLKEKLLAAKRNKV
                     QKVFLPEKNRPLYESISEEIRSGIKPLFVSEYHEVYEEL"
     gene            complement(2332..2799)
                     /locus_tag="PL056_08675"
     CDS             complement(2332..2799)
                     /locus_tag="PL056_08675"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HPM47130.1"
                     /translation="MTERKNSDNLSVLKQYRGLVEEIESALSDLLDPEKSQKSEIDFP
                     YDVFIDEGGCRLVVEVEVPGLTMENVSVFGFDQFIEISGDKDTLRSNRYENCVCLERE
                     NGTFRKVIHLGRAVNFNKATVSMDNGVLIIKFPIVVDKRGIIKIEPENGGEHE"
     gene            2960..3988
                     /gene="prs"
                     /locus_tag="PL056_08680"
     CDS             2960..3988
                     /gene="prs"
                     /locus_tag="PL056_08680"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HPM47131.1"
                     /translation="MSSDRGQLGIIACNSGRAFANSVVDYLKDKYKRDNREDRFRYVK
                     TTETHFANGEIKTSIDEPIRGMDVYIIQLMDDPMSDLSVNDNLMALATAVNAAYNSDA
                     GSITAVIPQFPNARQERRKGRESVTASMVSAFIEESGASRVLTIDIHAEAIAGFFHRA
                     RLDNLHASRQLMRAMCKRSLINDNTVVVAPDVGSAETARYYSRELKTDMALIVKERDY
                     SKASVVVEARLVGSVEGRNVVIVDDMIATGGTLIAACAKLKELGAENIDVFCTFPFFN
                     GDAVKKLDEAFANGTIHSVTGTNAVWRGEKFIADHPWYNEVSVTRLFAKVIYHINHME
                     SISNILES"
     gene            4013..4903
                     /locus_tag="PL056_08685"
     CDS             4013..4903
                     /locus_tag="PL056_08685"
                     /inference="COORDINATES: protein motif:HMM:NF013428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HPM47132.1"
                     /translation="MLRIDKVAGHDVLMESDIPFLKLFRKGKVREVYEIGEYLLIVAT
                     DRISAFDVVMKSGVPGKGVYLTKQSSYWFKFIEKNGIPTHFVTDNIAEMVEITGEKRL
                     LDLGWLEGRVMLGKKAEVLPVEAVCRFRIFGSAVIALKEGTWIWDEIESEEGLKEGAL
                     IKNGPVFTPTEKSETDDKISFEQMVELIKDRELAEKVKKNTIDLFRMCNDHAVSKFGF
                     EIADGKVEYGMIDGKLALVDETFTSDSARFIPDKSKEVFRKWLTDNNLKKVAAVVPDD
                     ISMKVSDLYRSQCNDMGIDI"
     gene            4922..6724
                     /gene="ftsH"
                     /locus_tag="PL056_08690"
     CDS             4922..6724
                     /gene="ftsH"
                     /locus_tag="PL056_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013906710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HPM47133.1"
                     /translation="MKFNYKTLLIWLGIIIAVFFVINISQMTAEKTIAEINMTEFRSK
                     VKAGEVKEVVIDGIEFKGKLNNDSTFVVIGPSGERMMEFLSENNVPTTYVKPEDNSFL
                     QTILASWLPMLIFIGLFIFFMRQMQGGNGKAFSFGKSKHRVISKDQNPIRFKDVAGVD
                     ESKEELTEVVDFLKRPAHYTKMGAKIPHGVLLVGPPGTGKTLLAKAVAGEADVPFFII
                     SGSDFVEMFVGVGASRVRDLFTHAKEKAPCIIFVDEIDAVGRQRGAGLGGGHDEREQT
                     LNQLLVEMDGFGSDLGVIVIAATNRPDVLDPALLRPGRFDRQVYVPMPDIKGREMILK
                     VHAEKIKMAEEINYHDIARGTPGLSGADLANLLNEAALIAASSSAKEVTMSHIEDARD
                     KILMGKERKTMVQTPEEKKDTAYHEAGHTIVGFYSKFADPIHKVSIVPRGRALGVTQH
                     LPEKDKYSYHKEELVDRIKILMGGRVAEELFIGRITTGAGNDIERATQLARNMVMQWG
                     MSENLGPIHYGEKEEQVFLGRELGKRTQVSEATSEKIDEEVQKLVDSAYQSAKSILET
                     HRDSVERLVAKLIEKETLEKNEIVAAIEGGQNEA"
     gene            6714..7409
                     /locus_tag="PL056_08695"
     CDS             6714..7409
                     /locus_tag="PL056_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47134.1"
                     /translation="MKLKVLFLTVAFVSLIFASCSTVPKKTINTDSSYSWTKESFELD
                     PQNQSFSAEGEVTMWIDSVQFKGKFNGDYQILSSERSKWRMIITGPFNITVATVIING
                     MAAHIFHDGKWESKPWPEISAGLFNADVDGDVLSVILGGRFKFDGECAEISENSKLCK
                     MNEIYYKFAGNQIVEILSGDLYAVRKDGSWTGVRNGKYAFIFKNKDFDNTVLLKDSLF
                     GLPVEEKDEFDDL"
BASE COUNT         2087 a         1495 c         1741 g         2108 t
ORIGIN      
        1 aacaactctt cgtaaacttc gtgatattcg ctgacaaaca ggggttttat tcctgatctg
       61 atctcttctg atatgctttc atagagaggt cggttctttt cggggaggaa tactttttgg
      121 actttatttc tttttgcggc aaggagtttt tccttgagtc cgccgatggg aagcacttct
      181 ccggtgagcg atatttcgcc tgtcattccg gtgaacgggt ctatctcttt tttaaggaat
      241 aagctcaaaa gtgcagtggt tatggttatt cctgcacttg ggccgtcctt ggggatcgca
      301 cctgccggaa aatggatgtg aatgtccttg tcttcagttg taaaatcgag tccgagtccg
      361 aaagtttcag cattgctctg aatatatgaa agggcgatcc ttgccgattc tttcattaca
      421 tcgccaagct gtcctgtgag gatcagcccc cctttgcctt tcatccagtt tgcctcgatc
      481 ttcagtgtgc ttccgccata ctgagtccat gcgagtccag tcacaacacc gattctcgca
      541 tcgtaatcaa gttctgtgtc tgaatatggt ggaagtttga gatatttttc aacaagtttc
      601 ggtgttatgg ttttcgggaa ctcttttttc tgaactttga gcgttgtcac ttttctgcat
      661 attgatccga tgttcctttc aagatttctg acacctgatt ctcttgtgta tcttcttgaa
      721 atgaaaagaa gtgcctcatt ggtgaacttc agctcttttt gctttaatcc gcagttttca
      781 agctgtctgg gaataagata tttttttgcg atctcaagcc gttccggatc ggtgtatccg
      841 gaaagacgga ttatctccat cctgtcttta agcggtgccg gaatggtctc ttcccagttg
      901 gctgtcgcaa tgaagaaaac ttttgaaagg tcaaaaggga tcccgatata gttgtcaagg
      961 aacgagaaat tctgttcagg atcaagcact tcaagcagag ccgaagatgg atctccgcgg
     1021 aaatccttgc caactttatc aatttcatca agcatgaaaa cggggttgct ggaaccgctt
     1081 gtctttattc cttcgataat ttttccgggc attgcaccga cataggttct tctgtgacct
     1141 ctgatctcgg cttcatcgtg aagtccgcct aagctcagcc ggtagaaatt tctgtcaagt
     1201 gctcttgcaa tcgatttccc gagcgatgtc tttccaactc cgggaggccc gatgaagcaa
     1261 aggatcggtg atttagagtt gccgcggatc tgtcttaccg caagaaattc aaggatcctc
     1321 tctttcacat caacgagccc gaaatggtct tcttcaagta ttttaccggc ttttgcaatg
     1381 tttttattat cttttgtctc ttccatccac ggaagttcga gaattgtatc aagccatgtc
     1441 ttgacaacga ctgtttcaga tgccgcaggg ttcattgttt ttaagcgttc aagctgtttt
     1501 tttgcttctg ttctcacctt ttccggcaat tcaatcgaat caagtttctt ttcgtattcc
     1561 tgaatttcag aaaggtgggg atcttcgttt ccaagttcct tctggatctg cttcatctgc
     1621 tctttgagaa aatattcctt ctgtgctttc ccaagccgtt cctttgcctt gttgcggatg
     1681 tctgattgta tttcgatgag cgaaagttct ttttcaagtg attcatgaac gatctgaagt
     1741 tttttcatca ctgagttctc ttcaagcact ctttgtgaaa gttcaacaga ttttgaaaga
     1801 tggctcatta ttatctctgc aagccggccc gggtttttca caccctgaat tgccgcaagt
     1861 gcttcaggag gaagcatgat ctcgccctga tcggcaatgt tctttatctg ccgttttatt
     1921 tcggtcatca atgcctgcga cataacattg ttctggtcgg gttcatcgtc gatcacattt
     1981 acatctgcag tataataacc gccctgttca tcataacgga tgtttcgaat ggacgctttt
     2041 ttgtatccct gaatgtaaag catcatgttt ccgtcaaagt tcttctgcat tctaataacg
     2101 gttgcaacgg tgcccgtaga atagactgtt tcgtccttta tctggtcaat tgagggatct
     2161 ttctgtgctg taacgatcag atatccttcc gcggttatcg ccctgttgac ggcgcttgag
     2221 gaaaaacccc gctggacatt gacattcatt atcatatatg gaaaaaggac tgaatctttc
     2281 agcggcagga ccgggagtga aggggggatt atgttcgttg ttccgtttcc gttattcatg
     2341 ttctcctccg ttttctggct ctattttgat aatacccctt ttgtcaacaa ctatcggaaa
     2401 ttttattata agcacgccgt tgtccattga aacggtcgct ttattgaagt ttacggccct
     2461 cccgagatgt attactttcc ggaaagttcc gttctcccgt tcaaggcaga cacagttttc
     2521 ataacggttg cttctcaaag tatctttgtc tccgcttatc tcaatgaact ggtcaaaacc
     2581 gaaaacactg acattttcca tcgtaagtcc cggcacttca acttcaacga caagccggca
     2641 gccaccctca tcaataaaca catcgtatgg aaaatcaatc tcgctcttct gagatttttc
     2701 agggtcaagc agatcggaaa gtgccgattc tatctcttcg accagtccac ggtactgttt
     2761 aagaacgctt aaattgtcag aattttttct ttcagtcata ttccaccttt ataaatcaaa
     2821 atccttgcca agtttacagg aatcgcattt tatggcaagt caaatcttat aaaaatattt
     2881 atcctaaaat cttgtaaata aagcgatgtg acattatata tatgcagtac tttgtttttt
     2941 ttattttccg gagggaaaaa tgagctcaga tagaggacaa ctcggtatta ttgcttgtaa
     3001 ttcaggcaga gctttcgcca attcagtggt ggactacctt aaagataaat ataaaaggga
     3061 caaccgtgaa gaccggttca gatatgttaa aaccacagaa acccattttg caaacggtga
     3121 gataaagact tcaatagatg aaccgatccg cggaatggat gtctatataa ttcagcttat
     3181 ggacgatccc atgagcgatc tttcggtaaa tgacaacctg atggctcttg ctacagcggt
     3241 gaatgctgcc tacaacagtg atgccggttc gataacggcg gtcattccgc agttccccaa
     3301 cgcaagacag gaaagaagaa aagggcgtga aagcgttaca gcttcaatgg tttcagcatt
     3361 cattgaggaa agcggtgctt cgagagttct gacgatcgac attcacgctg aagcgatcgc
     3421 cggttttttc catcgtgccc gtcttgacaa ccttcatgcc agcagacagc ttatgagagc
     3481 gatgtgcaag agatcactga taaacgataa caccgttgtt gttgcacccg atgtcggaag
     3541 tgctgaaact gcaagatact actcaagaga gcttaagaca gatatggccc ttatcgtaaa
     3601 ggaaagagac tattcaaagg cctctgtcgt tgttgaagca agactggtag gaagtgttga
     3661 gggaaggaat gttgtaatag ttgatgatat gatcgcaaca ggcggaacgc tgatcgcggc
     3721 atgtgcaaag cttaaagagc tcggtgctga aaatattgat gttttctgca catttccgtt
     3781 ctttaacggg gatgctgtta agaaactgga cgaggcgttt gcaaacggaa cgatccattc
     3841 tgttaccgga acaaatgcag tctggagagg agaaaagttc attgccgatc atccatggta
     3901 taacgaagtg agcgtgaccc gtctttttgc gaaggttatc tatcacataa atcacatgga
     3961 atctatttcg aacatacttg aatcttaaaa tatcttcagg gaggaagcag tgatgttaag
     4021 gatcgacaag gttgccggtc atgatgtgct tatggaatcg gacattcctt ttctcaaact
     4081 tttcagaaaa gggaaggtgc gtgaggttta tgagataggt gaatatcttc tcatagttgc
     4141 caccgacaga ataagtgcat tcgatgttgt aatgaagtcg ggtgttccgg ggaaaggtgt
     4201 gtatctgaca aaacagtcct catactggtt taaattcatt gagaagaatg gaattccaac
     4261 ccattttgtg acagataata ttgcggaaat ggttgaaata acaggggaaa aacggcttct
     4321 tgatcttggc tggcttgaag ggagagtgat gcttggtaaa aaagcagaag tgcttcccgt
     4381 tgaggcggta tgcaggttca gaatattcgg ctcggcagtt attgccctca aggaagggac
     4441 atggatctgg gatgagatcg aatctgaaga aggattgaag gaaggtgctc tcataaagaa
     4501 cggacctgtt ttcactccga cagagaaatc tgaaactgat gataaaattt cttttgaaca
     4561 gatggtggaa cttataaaag acagagaact cgctgaaaaa gttaaaaaga acacaataga
     4621 tcttttcagg atgtgcaacg atcacgctgt ttcaaagttc ggttttgaga tcgctgacgg
     4681 aaaggttgaa tacggcatga tagatggaaa actcgccctt gttgatgaga cattcacaag
     4741 tgacagcgca aggtttattc ctgataagtc aaaggaagta ttcagaaaat ggcttacgga
     4801 taacaatttg aaaaaagttg cggcagttgt gcctgatgac atttcaatga aagtttccga
     4861 tctttacaga agtcagtgca atgatatggg aattgatatt taaaagataa ccggagagta
     4921 catgaagttc aattacaaaa cacttctgat ctggcttggg ataatcatag ctgtcttttt
     4981 tgtgataaat atttcacaga tgacagcaga aaagaccatt gccgagatca atatgaccga
     5041 attccggtcg aaagtgaaag cgggagaagt gaaagaggtc gttatcgacg gcatagagtt
     5101 caaagggaag ctcaataacg actcaacctt tgtcgtcata ggtccgagcg gagagagaat
     5161 gatggaattc ctttccgaaa acaatgttcc gacaacttat gtgaagccgg aggacaattc
     5221 attccttcag acgattcttg catcatggct tccgatgctt attttcatcg ggcttttcat
     5281 ctttttcatg agacagatgc agggtgggaa cggaaaagca ttttctttcg gcaaaagcaa
     5341 gcacagagta atctcaaaag atcagaatcc cataagattc aaagatgtcg caggggttga
     5401 tgaatcaaaa gaggaactta cggaagtggt tgattttctt aaaagaccgg ctcattacac
     5461 aaaaatgggt gctaaaattc ctcacggagt tcttcttgtc gggcctccgg gaacagggaa
     5521 aactcttctt gcaaaagcag ttgccggtga agctgatgtt cctttcttca tcataagcgg
     5581 ttcagatttt gttgagatgt tcgtcggagt cggtgcaagc agggtcagag accttttcac
     5641 ccatgccaag gaaaaagcgc cgtgtattat tttcgttgac gaaattgatg cagtaggtcg
     5701 tcagagaggt gcagggctcg gcggcggtca cgatgaaaga gagcagacat tgaatcagct
     5761 gctggtcgaa atggacggtt tcggaagcga tctcggtgtc attgtcattg cggcgacaaa
     5821 cagacctgat gttcttgacc cggcactttt aagaccgggc agatttgaca gacaggttta
     5881 tgttccgatg cctgacatca aaggtcgcga gatgatattg aaagttcacg ctgaaaaaat
     5941 aaaaatggcg gaagagataa actatcacga catcgcaaga ggaactcccg gactttcagg
     6001 agccgacctt gcgaaccttc tcaacgaagc ggctctcatt gcggcatcaa gttctgcaaa
     6061 agaagttacg atgtcacaca ttgaagatgc gagagataaa attctcatgg ggaaagaaag
     6121 aaaaacgatg gtgcagactc cggaagaaaa gaaggatacc gcatatcacg aggcgggaca
     6181 cacaatagtc ggattttatt caaaatttgc tgatccgata cacaaagttt cgatagttcc
     6241 aagaggcaga gcgctcggag tgacccagca tttacctgaa aaggataaat acagttacca
     6301 caaagaagag cttgttgaca gaataaagat cctcatgggc ggaagagttg ctgaagaact
     6361 tttcatcggt cgtattacaa ccggagccgg aaacgacatc gaacgggcga cacagcttgc
     6421 aaggaacatg gtgatgcagt ggggaatgag cgaaaatctc gggcccattc attacggcga
     6481 aaaggaagag caggtgtttt taggccgtga acttggcaaa cggactcagg tgagcgaagc
     6541 aacatcagaa aagatagatg aagaagttca gaagcttgtt gacagcgcat atcagagtgc
     6601 aaagagcatt cttgaaaccc atagagattc cgttgaacgg ctggtggcta aactcattga
     6661 aaaagagacg cttgaaaaaa acgaaatagt tgccgcgata gaaggtggtc agaatgaagc
     6721 ttaaggttct gttcctgacg gtggcatttg tttctttaat ttttgcatca tgctcaacag
     6781 ttccgaaaaa aacaataaat acagatagtt cctatagctg gacaaaagaa agttttgagc
     6841 ttgatccgca gaatcagtcg ttttctgctg aaggtgaagt gacgatgtgg atagactctg
     6901 tccagttcaa agggaagttc aacggtgatt atcagatact ttcaagcgaa aggagcaaat
     6961 ggcggatgat catcacaggc cctttcaata taacagttgc aacggtcatc atcaatggaa
     7021 tggctgcaca catctttcat gacgggaaat gggaaagcaa gccgtggcct gaaatttcag
     7081 cagggctttt caatgccgat gttgacgggg atgtcctttc ggtgatactt ggcggaaggt
     7141 tcaagtttga tggcgaatgc gctgaaatat cagaaaattc aaagctttgc aaaatgaatg
     7201 agatctatta caagtttgca ggcaaccaga tcgttgaaat attaagcggt gatctctatg
     7261 ccgtaagaaa agacggctcc tggaccggag tcagaaacgg aaaatacgca ttcatcttca
     7321 aaaataagga tttcgacaac acagttttgc tcaaagattc attattcggg ctccccgtgg
     7381 aagaaaaaga cgaattcgac gatttgtgat aatcttaacc gattttttac a
//
LOCUS       DAOOYB010000137         7388 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_11340_length_7388_cov_9.858312, whole genome shotgun sequence.
ACCESSION   DAOOYB010000137 DAOOYB010000000
VERSION     DAOOYB010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7388
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11340_length_7388_cov_9.858312"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2134
                     /locus_tag="PL056_08700"
     CDS             <1..2134
                     /locus_tag="PL056_08700"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HPM47135.1"
                     /translation="PDVENMLPEYMSVHLKELEEKFGGMEIIFVLVESDDILRPSVLE
                     HINSIADILKNVQGIERINSIPMSDDDPFDDEKLSEGSAQELQAREKLRNMLKNNNMI
                     MNTLVSEDFKAASILVRMKNEGKGLEITRKVKEETLKIQGPGKISFGGMPVIKEHISE
                     DVPRDMMIFMPLGLLIMLGVLMMSFKQLRGVLLPFLVVIMSIIVSMGLVPLLGWKMTT
                     VTIILPVILIAIANGYGIHIISRYQLDNMVAGGKKSSLELSKDVFRELAIPVLLTGVT
                     TIAGMLCLMTHTVVPAAQLGILSSVGIFFALMASIFFIPAVLSALDAPKPVIDKEKRP
                     ATEKMLKKLSNFLSLHPKKLLSASIVLTTIMAAGIPLIIVDSNLAGYYPEDHPVRKSA
                     KLINEKFGGSQVISINVDGDIKDPQVLRKIDETEKKISLHKNVGNTLSIAKLLKLITK
                     GAYKPEEKGFGQIPETKNAVEFFLDKYNEMGDPEELRKLVNNDFTQAQIIAQINSESS
                     ETIKKVVEDTQKLIDDDPVFTKITGSGVIFKDLIEKVIEGQVISLFLSFFEVSFLVML
                     LFKSIVAGIIAGIPLAVSLVLMFGFMGFTGVTLDIATALISSIVIGVGVDYTIHFLWR
                     YKIEMSKHNDPVMAVDHTLSTTGRGIIFNALSVMVGFVVLLFSSFLPIRFFGTLIFLS
                     IAVCLIGSIVLLPSICIVFRPKFLEKKD"
     gene            complement(2136..2273)
                     /locus_tag="PL056_08705"
                     /pseudo
     CDS             complement(2136..2273)
                     /locus_tag="PL056_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005913562.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
     gene            2865..3734
                     /locus_tag="PL056_08710"
     CDS             2865..3734
                     /locus_tag="PL056_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47136.1"
                     /translation="MRLLSAIITCCMVFALSCGSADSKDDKTSMLEKKIEMIKADNKE
                     KLVTTMVKVFELKSENEKFINETIMDMQKEILKKVTPHGLDKFKESPNRDLKIPSNVS
                     QIHLKNNYPSGNAVVFLMKDGDPSIIDVFEFNTEKKYEVEPGNYSFIMTIDRKFNNNI
                     FVFKSDLNFRGGELYLGEFNINSQYNKSGSCEQGFIPDDDMCIIPSFKILDNCNPGSH
                     LVEHLCCEEGFNFIMEGKCSRYSDAVESVICPAGYHEGGKGRCCPKGMLFIDDQCQVP
                     PKDAPAPAPEQNQ"
     gene            3780..4073
                     /locus_tag="PL056_08715"
     CDS             3780..4073
                     /locus_tag="PL056_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47137.1"
                     /translation="MFRDFLQSELELNGLPMVKELTYRFAMVQFRDTRDFEPGKIEFI
                     PKVLAHMQSEMGEIFEKFAKECFDHGYNLDFINETVRQWLIKIINEIVPAQQV"
     gene            4025..5074
                     /locus_tag="PL056_08720"
     CDS             4025..5074
                     /locus_tag="PL056_08720"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HPM47138.1"
                     /translation="MAYKNYKRDRSGTTSLIPFVVFSLLFHGILLFVKIDAGSSSDDS
                     TVAIKYYDLNDATPVPPEKAVPEKPGEDLLKGQVVDIARPEIQKKPEKSRYLSEYDSS
                     VSKETKSDFDNNINIRDKKLRKKLSETIQRETDTEERKEDVVSGPENDALTMRQEKVK
                     KGEMKGFEGSDGKYLRGGEKEKSGLVNAEDALDQEGSQMIGGHRIPNRFLPYMNGNDT
                     LLTTPSNDFLKDVEKGDETALNARKFIYSAYFNKIKQAISQHWTPSYVLMINDPGGHV
                     YGKKDRLTKLIVHISSSGTLISAKVDVSSGIDFLDREAINAFKMASPFSPPPEVLLNS
                     QNLLEIHFSFMVIVE"
     gene            5074..5745
                     /gene="queC"
                     /locus_tag="PL056_08725"
     CDS             5074..5745
                     /gene="queC"
                     /locus_tag="PL056_08725"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018046648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HPM47139.1"
                     /translation="MCEKAVVLFSGGLDSTTVLAYAVSKGFDITPLTFSYGQRHSIEV
                     EKASLALRKYGLWNKSAVFNIDLSVFSNCSLLNSGIEIPKDPDKGIPSTYVPSRNLIF
                     LSIAAGFAETIDASRIFIGVNSVDYSGYPDCRPEFIRKFNETVSVGTKKGVESSIIVE
                     APLIDMSKKEIIQLGISLGVDYSMTHSCYNPSENGLSCGVCDSCRLRLEGFRQAGMKD
                     PLDYI"
     gene            5797..7290
                     /locus_tag="PL056_08730"
     CDS             5797..7290
                     /locus_tag="PL056_08730"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HPM47140.1"
                     /translation="MRKLLIVIFILPFVMIIAGCGGEDDETESGNKKECFSNNDCAVG
                     FECNLSTYQCEQSGDSGPLPDNSDSGDDGDSGDSGDSGNSGDTGTTTGDCTPDQTQKC
                     NYQGSPITEDIGPCKAGVRTCKSDHTWGPCEGEVLPEFEVGELCTDGIDNDCDGVEDN
                     GADIDGDGVPSCQDCCEYAKDCPDPKDAWDATMHFCSYDETENAQLYECDSSLNGATK
                     EPMDYAKAIGLCKTATDDPSSGWGVISAEILKPDGSFGAHANSSALLNALGNVIKPVA
                     GSFMTALSTGEAVNPFPGSHKSMSGSSSAPADWYAANGNSLPNAPGCPNATSINDAIM
                     LKLRVRVPNAAKSFSFNMNFLSVEYPTYVCTKYNDFFVALLDSTYNQQNPTSELKNPE
                     DKNLAMDENGYPVGVNLAKSGLFKICTVTASYPSCAGDADLNGTGGLEGHGGTGWLVV
                     RGNVVGGEVITIRLATWDSSDQIWDSIVLIDNFKWEFEEYKPGTGAS"
BASE COUNT         2271 a         1324 c         1614 g         2179 t
ORIGIN      
        1 ccctgatgtg gagaacatgc ttcctgagta catgagtgtt catttaaagg aacttgaaga
       61 gaagttcgga gggatggaga tcatttttgt tcttgtggaa tctgatgata ttttgagacc
      121 ttcagttctt gaacacatca attcaattgc agacatcctg aagaatgtgc agggcattga
      181 gcggattaat tcgattccga tgtcagatga tgacccgttt gatgatgaaa aactgtcaga
      241 aggatctgct caggaacttc aggcgagaga aaaactccgg aatatgttga aaaacaataa
      301 tatgataatg aacactctcg tttcagagga ttttaaggcg gcatcaattc ttgtccggat
      361 gaaaaatgaa ggaaaggggc ttgaaataac acgaaaagta aaagaagaaa ccctcaagat
      421 ccaaggtccc ggcaaaataa gtttcggagg aatgcctgtt atcaaggagc atatttcaga
      481 agatgttcca agagatatga tgatctttat gcctttaggg cttcttatta tgcttggtgt
      541 tctgatgatg agcttcaagc agctcagagg tgtgttactt ccatttcttg ttgttatcat
      601 gtccataatc gtttcgatgg ggcttgtccc gcttctggga tggaaaatga ctacggtcac
      661 aataattctt ccagtaattt taatagcaat tgcaaatggt tacggaatcc acataataag
      721 cagatatcag cttgacaaca tggttgccgg agggaaaaaa agctcactgg aactctctaa
      781 agatgttttc agagaacttg cgataccggt acttctgaca ggtgtcacaa caattgcagg
      841 aatgctatgt cttatgacac acaccgttgt tccggcggca caactcggga tactctcctc
      901 tgtaggaata ttttttgctt tgatggcaag tattttcttt attcccgcag ttctttcggc
      961 acttgatgct cctaaaccgg taattgataa agagaaaaga cctgcaacag agaaaatgct
     1021 taaaaaactt tcaaattttc tttcgttaca tcccaagaaa cttctttcag caagcattgt
     1081 cctgacaacc atcatggctg ccggcattcc tctgataatt gttgactcca accttgccgg
     1141 ctattatccg gaagatcatc ctgttagaaa atcggcaaaa cttataaacg aaaaatttgg
     1201 tggctctcag gtgataagca tcaatgtgga tggtgatata aaagatccgc aagttcttag
     1261 aaaaattgat gaaactgaaa agaaaatttc gcttcacaaa aatgtaggga acactctttc
     1321 gatagcaaaa cttttaaagc ttattaccaa gggcgcatac aagccggaag aaaagggatt
     1381 cggacaaata ccggaaacaa aaaatgcagt tgagtttttt ctcgataaat acaatgaaat
     1441 gggagatcct gaagagttga gaaaacttgt caacaatgat tttacacagg cacagataat
     1501 cgcacagata aacagtgaaa gcagcgaaac tattaagaaa gttgttgaag atacccagaa
     1561 actgattgac gatgaccctg ttttcacaaa aataaccggt tccggagtaa tatttaagga
     1621 cctcattgaa aaagttatag aaggacaggt aatatcgctt tttctttcgt tttttgaggt
     1681 ctcatttctt gtaatgcttc tttttaaatc gatcgttgca ggaataattg caggcattcc
     1741 tcttgctgtt tcacttgtcc tgatgttcgg atttatggga tttaccggag tcacccttga
     1801 cattgcaaca gcacttattt cttcaatagt cattggtgtc ggtgtggact atacgatcca
     1861 ttttctatgg agatacaaga tagaaatgtc caaacataac gatcctgtta tggcggtgga
     1921 ccacactctt tctacaacag gaagaggaat aatatttaat gctttaagtg ttatggtggg
     1981 ttttgtcgtt ctgcttttca gctcatttct tcctataagg ttttttggaa cgctgatatt
     2041 tttaagtatt gctgtatgtt taatcggttc gatagtcctt cttccttcaa tatgtatagt
     2101 tttcaggccg aaatttctgg aaaagaagga ttaaactatt ttccgcttaa aaaacttaaa
     2161 ttttttatat tatactttgt gctgttctga cgaagtttct gcaatgctct catttcaacc
     2221 tggcggatcc tttccctggt aaggttcatt atcgcaccca cttcttcaag tgttactcca
     2281 cctttatcag cgacatcaag agcacatgtt tccctgagtt cccacacttc tttacccggg
     2341 aagttgaatt tgatcgattt tgtttccggg ttcacatcaa ggaaaagatg atatttgcat
     2401 gaaacaaaaa gacacggcct tttaagtttt aggcattcag atctgttttt gggtttctga
     2461 taggaggtta tgtcgataag atctctgtat atcggatctt tttcgagttc gtttctctga
     2521 tcgaaaacca cttcggtgta gggtttaact ttcttttttg atttttcacc tttcgggaac
     2581 atttgcttcc tcctgtgtta ccatatgttt cacacatgac cgccaattgc agaatgatat
     2641 tatgactcta aatcaatgtg tcaagcacaa aaatcataat atgttgaata tattggataa
     2701 aaaaaacatt attcaggtag tgtataatat gtcgttataa aaaatcgcat aaatttattg
     2761 gcaaaaaagg ttgatttaat aattataaga ctgtatcatt taacacgaaa aagattcgct
     2821 ttgtgaaatg tgagttttaa taattttttt gtgaggaaga cgaaatgaga cttttatcag
     2881 caattataac atgttgtatg gttttcgctt taagttgcgg aagtgcggat tcaaaagatg
     2941 ataaaacatc tatgcttgaa aagaagatcg agatgatcaa agctgataac aaggaaaaac
     3001 ttgttacgac aatggtgaag gtgttcgagc ttaaatcgga aaatgagaaa tttatcaatg
     3061 aaacaataat ggacatgcag aaagagattc taaaaaaagt gacacctcac ggacttgata
     3121 aatttaaaga atcgcctaac agagacctta aaattccgtc aaatgtttct cagatccatc
     3181 ttaaaaataa ttatcctagc ggaaatgcag tggttttcct tatgaaggac ggagaccctt
     3241 caattataga tgtgtttgaa ttcaacacag aaaaaaaata tgaagttgag ccgggcaatt
     3301 acagtttcat tatgacgatt gaccgtaagt tcaacaacaa tatattcgtt tttaaatcgg
     3361 atcttaattt ccgcggcgga gaactttatc tgggtgaatt caacataaac agccagtaca
     3421 ataagtccgg aagctgtgag cagggtttca ttcctgatga cgacatgtgc attatccctt
     3481 cgttcaagat acttgacaac tgtaatcccg gaagtcacct tgtagaacat ctctgttgtg
     3541 aagagggatt taatttcata atggaaggaa agtgctcaag atacagtgat gctgttgaaa
     3601 gcgttatctg tcctgccggt tatcatgaag gtggaaaggg aagatgctgc ccgaaaggga
     3661 tgcttttcat tgatgatcaa tgccaggttc cgcctaaaga tgctcccgca cctgcacctg
     3721 aacagaatca ataattcttt aaaatggaac ggaacactga tttcgaaaga tattttgtca
     3781 tgttccgcga tttccttcaa agtgaacttg aactgaatgg tctgccgatg gttaaagaac
     3841 tgacataccg ttttgctatg gtgcagttca gggatacccg tgactttgaa ccggggaaga
     3901 tagaatttat tccaaaagtt cttgctcata tgcagtcgga aatgggggag atatttgaga
     3961 aatttgcaaa agaatgtttt gatcatggat acaatcttga ttttattaat gaaacggtta
     4021 ggcaatggct tataaaaatt ataaacgaga tcgttccggc acaacaagtt taattccgtt
     4081 tgtcgtattc tcactccttt ttcacggaat tctgctgttt gtgaaaattg atgccggctc
     4141 atcctctgat gattcaaccg ttgcaataaa atattatgat ttaaatgatg cgacacctgt
     4201 gccccccgaa aaagctgttc ctgaaaaacc cggagaagat cttttgaaag gacaggtcgt
     4261 cgatattgca agaccggaaa tccagaaaaa accggaaaag agcagatatc tttcagaata
     4321 cgacagttct gtctcaaaag aaacaaagtc cgattttgac aataatatca atatcagaga
     4381 taaaaaactc agaaaaaagc tttctgaaac cattcaaaga gaaacagaca cagaagaaag
     4441 aaaagaggat gtcgtatccg gtcctgagaa tgatgctctc acgatgcggc aggaaaaggt
     4501 caaaaaaggt gagatgaaag gttttgaagg tagtgacgga aagtatctcc gtggcggtga
     4561 gaaggaaaaa agcggactgg tcaatgctga agatgcttta gatcaggagg gttcgcagat
     4621 gataggtggt cacaggatac cgaatagatt tctcccgtat atgaacggaa atgatacgct
     4681 gcttaccaca ccctcaaacg attttcttaa agatgttgaa aagggtgatg aaacggctct
     4741 aaatgcaaga aaattcatat attcagctta ttttaataaa attaagcagg cgatctcaca
     4801 gcattggacg ccgtcatatg ttcttatgat aaatgatccg ggaggtcatg tttacggaaa
     4861 gaaggaccgg cttacaaagc ttattgttca catttcttcc agcgggacgc tcatcagtgc
     4921 taaggttgat gtgtcgtcag ggatagattt tctcgacagg gaggcgataa acgcatttaa
     4981 aatggcatca cctttttcgc cccctcctga agttcttctg aatagtcaaa atcttcttga
     5041 gatacacttc agctttatgg taattgtaga atgatttgtg aaaaagcagt cgtacttttc
     5101 agtggcggac tcgattcaac gacagtactt gcctatgcag tttcaaaagg ttttgatatc
     5161 actcctttga ctttttcata tggacagcgt cattcgatag aagtcgaaaa ggcctcgctg
     5221 gcattgagaa aatacggtct ttggaataaa tcagccgttt tcaatatcga tctttctgtt
     5281 ttttctaact gctctctgct gaatagcggg atcgaaatac ctaaagatcc ggataagggg
     5341 attccttcga catatgttcc ttcacggaat ctaatatttc tttcaatagc ggcaggattt
     5401 gccgaaacga tagatgcatc aagaattttc ataggtgtca attctgtaga ttattcaggt
     5461 tatcctgatt gtaggcctga atttataaga aaattcaacg aaactgtttc tgttggaaca
     5521 aaaaagggtg tcgaatcgtc tataattgtt gaggccccgt taattgacat gtcaaaaaaa
     5581 gagataattc agctggggat ttcactggga gttgattatt caatgactca cagttgctac
     5641 aatccgtctg agaacggttt gagttgcggc gtctgtgaca gttgccggct ccgtcttgaa
     5701 ggtttcagac aggccggaat gaaagaccct cttgattata tttaaaaatt attgataatt
     5761 ataactagtg tcggtttttt acttgaggag aaaaagatga gaaaattatt aattgtcatt
     5821 ttcattctgc ccttcgtcat gatcatcgcc ggttgcgggg gtgaagatga tgaaacagaa
     5881 agcggaaaca aaaaggaatg tttttccaat aacgactgtg ctgtaggttt tgagtgtaat
     5941 ctgtctacat accaatgtga acaaagcggt gattcaggtc ctcttccgga caattccgat
     6001 tcgggagatg atggtgacag cggggattcc ggagattcag gaaattcagg agatactggt
     6061 accacaacag gagactgtac gcctgaccag acccagaagt gtaattatca gggatctccg
     6121 ataactgaag acataggtcc atgcaaagct ggagtgagaa catgcaagag cgaccataca
     6181 tggggtccat gcgaaggaga ggttcttcct gaatttgagg tcggggagct ttgtacagac
     6241 ggcatagaca acgattgtga cggtgttgaa gataatgggg ccgatattga cggtgacgga
     6301 gttccaagct gtcaggactg ttgtgaatat gcgaaggatt gtccagaccc gaaagatgca
     6361 tgggatgcca ccatgcattt ctgctcttat gatgaaactg aaaatgctca gctttatgaa
     6421 tgtgacagtt cattgaacgg agcgacaaag gaaccgatgg attatgcaaa agcgattggt
     6481 ctttgcaaaa ctgcaacaga tgatccgtca agcggatggg gtgtcatcag tgctgaaata
     6541 ctcaagcctg acggaagttt tggagcacat gcgaatagca gtgctttatt aaatgctcta
     6601 gggaatgtca tcaagcctgt agccggttct ttcatgacag ccctttcaac aggtgaagca
     6661 gtaaatccat ttcccggttc tcataaaagt atgagcgggt caagttctgc tcctgccgac
     6721 tggtatgcag cgaatgggaa ttcacttcct aatgctcccg gttgtccaaa tgcgaccagt
     6781 attaatgacg cgataatgct gaaactaagg gttagagttc ctaatgctgc taaatcattc
     6841 agctttaaca tgaattttct ttctgtcgaa tatccgacat atgtctgcac caaatataat
     6901 gacttttttg ttgcccttct tgattcgact tacaaccagc aaaatccgac ttcagaatta
     6961 aaaaaccctg aagataaaaa tctggcaatg gatgaaaatg gttatcccgt aggagtaaat
     7021 cttgcgaaat cagggctttt taagatttgt acggtaactg cttcttatcc gtcatgtgca
     7081 ggagatgcag atctcaatgg aacaggtgga ctggaaggcc acggtggaac tggctggctt
     7141 gttgtgagag gaaatgttgt gggtggagaa gtgataacta ttcgtcttgc cacatgggat
     7201 tcaagtgatc agatctggga ttccattgtt cttattgata acttcaagtg ggagtttgaa
     7261 gaatataagc ccggtacagg cgcttcatag atcagaaatt tttctcaaat cttttccaat
     7321 tgttaaaagt ctgttaattc tttcagatgc cattgcgtat tccgttaaaa aaattaatgt
     7381 gagaaaag
//
LOCUS       DAOOYB010000138         7330 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_11459_length_7330_cov_6.772921, whole genome shotgun sequence.
ACCESSION   DAOOYB010000138 DAOOYB010000000
VERSION     DAOOYB010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7330
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11459_length_7330_cov_6.772921"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="PL056_08735"
     CDS             complement(<1..527)
                     /locus_tag="PL056_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011713828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HPM47141.1"
                     /translation="MINFKEDRSQFPEKNPDGTFKIDLASHKFSHIWQGPQHPGITGN
                     MSLELIIQGDEVMSCKTHVGYLHRGFEKLMERRKYIQCFPLVCRICVPEPDFNEHCFA
                     TAVEELAGITVPEKALWLRTLTLEMARTASYLMWFGGAAGSLGMGIIGQWTNYLRDYI
                     LDRFEELTGGRIYHM"
     gene            complement(529..1062)
                     /locus_tag="PL056_08740"
     CDS             complement(529..1062)
                     /locus_tag="PL056_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011713829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HPM47142.1"
                     /translation="MNNIFKQLETLFAVSDFEPKREKLAFLTVEKRHLVSALKFAKDN
                     LGFGHLILITAVDWLENGFFQITYLIKNNSNQFELGFKVFVPRNEPVMESVHDLWKAA
                     ATYQRELKEMFGIDFPGSPGVDESFILEGWHDIPPYRRDFDTKKYSEETYFPRPGRVT
                     HDPEEHMKKKLYPQREG"
     gene            complement(1071..1712)
                     /locus_tag="PL056_08745"
     CDS             complement(1071..1712)
                     /locus_tag="PL056_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002707157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HPM47143.1"
                     /translation="MSMDDLKKRKVTGPDGEEFEVDPLNDYFCTERPHPEYPGNKIVE
                     KFLNWARAESIWILGFGTGCGAIEMRPLMTPRFDAYRFGIQWRPTPRQSNLLVVSGYL
                     SVKTLKRVIRSYEQMQNPKYVMGLGSCTINGGMYWDSYNTINRLDHYLPVDTYIAGCM
                     PRPESLIAGFEQLKKIIKEGKGEGANKYIENLDWYKQNQKKIIKGWDMPDYNW"
     gene            complement(1717..2625)
                     /locus_tag="PL056_08750"
     CDS             complement(1717..2625)
                     /locus_tag="PL056_08750"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011713831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HPM47144.1"
                     /translation="MKFEWIKILYAVWALLIILNIGLVFQGVTQKIAARLGRRYGIRW
                     YQPYIDLMKNYAMRSSITHGVMFYLGPVFRLSGGIGMFLFMPVIFDSPYFQNFSFSGD
                     LILISYFMFFGTLGMALGAGEGGHPYSAIGITRGLSQMTAAEVPQILAIISVAVQYQT
                     ISITNIVAAQQGGIMNWTVMTNPFATAAALLALLGSMMRPPFDVVLAPQEIPIGPPTE
                     FHSNFLAVLQTNRAIFPMAKMVLFVNIFFGGATNWPLMITKMFLLYMWPIFIGVAFPR
                     FRVDQSIRWFIKIPAVIGIIAVLLVR"
     gene            complement(2622..5756)
                     /locus_tag="PL056_08755"
     CDS             complement(2622..5756)
                     /locus_tag="PL056_08755"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HPM47145.1"
                     /translation="MITPIYIIILSLGTAFLTAILSKKSEKLPFVMVFTAIMSITAIS
                     AYWAFRFIAFKDGSMIESTAGFSAPLSIALKVGIEESFALLVINLTALLGMPHIFKLI
                     KENGKYSAITYLLYFMALNGIVMTRDLFNYFVFIEISSIAVAGMLLFQKSGKLLKAGF
                     SWLVATGIISIFLILGAITAYVKTGVLDIDTIVLMNSSGLGTGSIAAFFLFIALIFEL
                     KPFPANRWAVDIYKSAPAGITAAISGAGMTASLFALYKFLPVLGPSWNSAVAVTGLLT
                     FVMSNLLAIKTENTNKMLGLSSIAQGGLATAVIGLGNILGTDTFFIAGTIVAVHAVSK
                     TGLFWLNGIAGFEKTKEWGKLKGNPFVIFLLGLFIFSLLGLPPFPTFFAKWMLIEQLA
                     GTSNWLWMALILIGSLLEAVYLIRWFGYSVKMESDTQKEIKFKELAMLFVSVAILFII
                     SIDIPFAVGHMPLLKTVSIPLIFATSFFILDFLPAKIKNILLISGLGYYFYHLYPVLF
                     EFRAVFLIVFLIGGMILLLAGFDKSGKRVGFYPSSALMYAGLAGLLTADTTMEFFFWW
                     ELMTLGSYFLILRGKKSMAHALSYMTFSLGGAYSMLAGFGLISAANNGNLELATLSTA
                     GAYQSVIFILFVIGFLTKTAAAPLHIWLPGAHSEAESDVSPVVSAVLLKAGVFGLIIT
                     MLAMGPQNFKGIDIYYVLGWLGVITALLGNMMAAFEEDAKRLLAYSSVGQLGGVVFAL
                     SMMNHLGWLTATTFALNHFVFKALLFLAIGGVVMRVKTRNMYEMGGLITRMPISFLSV
                     MIGIIALSGVPPLTGFGGKWLSYNAMIDSQMYFQGAVASFAGLVAFLYCFRLIFAIFL
                     GQLKDNHRHVKEAPFWMLVPQFTLLGLVMFFSVVPGVALKPIGEFLVKYFPENPLVWD
                     GYLAKSYLGYWDGKMIMYVTMGIFSVVFAWLFFINRKAVKVKQFNIVYAAERPSRPEL
                     THFAYNFFAPYRKALGFLVTPLATAFWNGASEGFHSTATFTSKIFKGNSQSYAIHILL
                     YAVISFIITFGGVQ"
     gene            complement(5753..6106)
                     /locus_tag="PL056_08760"
     CDS             complement(5753..6106)
                     /locus_tag="PL056_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007608141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HPM47146.1"
                     /translation="MAVTGIITGSLLILIGFYAILSKNNLMKMVIGFSLADTGVHIII
                     VALGYLKGRTAPILDNALLAANPETLVVDPVPQALVLTAIVIGFGITAIMLVFVWRLY
                     EKYGTLEISRIGEAK"
     gene            complement(6108..6791)
                     /locus_tag="PL056_08765"
     CDS             complement(6108..6791)
                     /locus_tag="PL056_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002707153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MnhB domain-containing protein"
                     /protein_id="HPM47147.1"
                     /translation="MVRNLFIIFILAGVASMFAALALGYSDPVALGSAASHYVSKGLA
                     ELNGVNIVTSVVVTYRGLDTLGEVTVLFLTASILSFFLKNRSHETKSESSELLTTASK
                     VIFPFIFMTGLYIVMNGHLTPGGGFQGGAVMASALLLLILADNKTDGSPTLFTVLESV
                     SGIFYVGTGVAGLILAGGFLDATVLGAGITGRLLSAGAIPLISTLIGLKVGSELSTML
                     VKLKNASEV"
     gene            complement(6784..7029)
                     /locus_tag="PL056_08770"
     CDS             complement(6784..7029)
                     /locus_tag="PL056_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4040 domain-containing protein"
                     /protein_id="HPM47148.1"
                     /translation="MELVILIVLSLVVIFSAVFAVIVKTLTSAVIASGAVGLFASVMY
                     IIYDAPDVALTEAAIGSGLMTVVFFLILSRMRRDENG"
     gene            complement(7030..>7330)
                     /gene="mnhG"
                     /locus_tag="PL056_08775"
     CDS             complement(7030..>7330)
                     /gene="mnhG"
                     /locus_tag="PL056_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007608138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HPM47149.1"
                     /translation="LTGSFFLFTASIGLIRMPDFFNRIQAGTKASTLGSLLILAGVAV
                     LNPVWWPKLLIIFIFIVITNPVSSHCIGRAGYHSNCEKGKLNRDDLKNLKINKGE"
BASE COUNT         2204 a         1645 c         1556 g         1925 t
ORIGIN      
        1 tacatgtgat atatcctgcc acccgtaagt tcttcaaatc tatcaagaat atagtctctt
       61 aaatagtttg tccactgacc gattattccc attccaagtg agccggccgc ccctccgaac
      121 cacatcagat agcttgcagt acgggccatt tcaagagtca gggttctaag ccacagcgct
      181 ttttcaggga cggtaatccc tgcgagttct tcaacagcag ttgcaaaaca gtgttcattg
      241 aaatccggtt caggaacaca tattctgcaa actaatggaa aacactgaat atattttctt
      301 ctttccatca gtttttcaaa acctctatga agatatccga catgggtttt gcaactcata
      361 acctcatcac cctgaataat gagttcaagc gacatatttc cagtaatacc gggatgttga
      421 ggtccctgcc agatgtgact gaacttgtga ctggcaaggt ctattttaaa tgttccgtca
      481 gggtttttct cagggaactg acttctgtct tctttaaaat ttatcatatc agccctccct
      541 ttgaggataa agtttctttt tcatatgttc ctcaggatcg tgagttactc ttccaggtct
      601 tggaaaatat gtttcctcag agtatttttt tgtatcaaaa tctctacgat acggtggaat
      661 gtcatgccac ccttcaagga tgaaagattc atcaactccc ggacttcccg gaaagtcaat
      721 tccaaacatt tctttaagct ctctctgata tgtcgccgcc gctttccaaa gatcgtgaac
      781 cgattccatc accggctcat ttctcggaac aaacaccttg aaaccgagct caaattgatt
      841 cgaattattt tttatcagat atgtaatctg aaaaaagccg ttttcaagcc agtctacagc
      901 cgttatcaga atcagatgac caaagccgag attgtctttt gcgaacttta aagccgaaac
      961 aagatgcctt ttttcaactg taagaaaagc gagcttttcc cgttttggct caaaatctga
     1021 aacggcaaaa agggtttcaa gttgtttaaa aatattattc atgtctcctc ctaccagtta
     1081 tagtcaggca tgtcccagcc cttaattatc tttttctggt tttgcttata ccagtcaaga
     1141 ttttcgatgt acttgttagc cccttcgcct ttaccttctt taattatctt ttttaattgc
     1201 tcaaatcctg cgataagcga ttcgggtctt ggcatacagc cggcgatgta ggtgtcaacc
     1261 ggaagatagt ggtcaagccg gttaatggtg ttgtaactat cccagtacat tccgccgttg
     1321 atcgtgcaac tgcctaagcc cattacatat ttaggattct gcatctgttc ataactgcga
     1381 atgacccttt ttaatgtttt cactgaaaga tagcccgaaa caacaagcag attcgattgt
     1441 ctgggggtcg gtctccactg aattccaaac ctgtatgcat caaatctcgg agtcatcagc
     1501 ggtctcattt caattgcgcc gcagccggtt ccgaaaccga gaatccatat tgattcagct
     1561 cttgcccagt taaggaactt ttcaacaatt ttattgcccg gatactcagg gtgaggcctt
     1621 tcggtgcaga agtaatcatt caatggatca acttcaaatt cttcaccatc gggacctgtc
     1681 actttccgtt ttttaagatc gtccatactc atgttttcac ctcacaagta atacagcgat
     1741 tattccgata acggcaggga tcttgataaa ccagcggata gactggtcaa ccctgaacct
     1801 cgggaatgcg acaccgatga aaatgggcca catgtagagc agaaacattt ttgtgatcat
     1861 caacggccag ttagtagctc cgccaaagaa aatgttcaca aaaagaacca ttttcgccat
     1921 agggaatatc gcacggttgg tctgaagaac ggcaagaaaa ttggaatgga actcagtcgg
     1981 agggccgata ggaatttcct gaggggcaag aaccacatca aaagggggcc gcatcatcga
     2041 accgagcaga gcaagaagtg ccgcggcagt tgcaaacgga tttgtcatga cagtccagtt
     2101 catgatcccg ccctgctgag cggcaacaat atttgtgatg gagatggtct gatattgaac
     2161 agcgacagag attatcgcaa gtatctgagg cacttcagcc gcagtcatct gcgaaagtcc
     2221 tcttgtaatt ccgatggcag agtagggatg tccgccttca ccagctccca gagccattcc
     2281 aagggttccg aagaacatga aatagctgat gagtatgagg tctcctgaaa atgagaagtt
     2341 ctgaaaatag ggtgagtcga atataacggg catgaagagg aacatgccga tgccacccga
     2401 tagacggaat accggcccga gatagaacat cacaccgtgt gttatggatg atctcatggc
     2461 atagtttttc atcagatcaa tatacggttg ataccagcgg atgccgtacc ttcttccaag
     2521 ccgtgccgca attttctgag taactccctg aaatacaagc ccgatattca atattatcaa
     2581 aagtgcccaa actgcataaa gaatttttat ccattcaaat ttcattgaac acctccgaaa
     2641 gttatgatga acgatatcac agcatacaga aggatgtgta ttgcataact ctgtgaattt
     2701 cctttgaaaa tctttgatgt aaatgtcgcg gtagaatgaa atccctcact tgcgccgttc
     2761 cagaaagcag ttgcaagagg agtcacaaga aatccgagtg cttttctgta aggagcaaaa
     2821 aagttgtatg caaagtgggt gagttcaggc ctgctcggtc tttcggcggc ataaacaatg
     2881 ttaaactgtt tgaccttgac cgcctttctg ttgataaaga aaagccatgc aaaaacaacg
     2941 ctgaaaatgc ccattgtgac atacattatc atttttccat cccagtaacc gagataactt
     3001 ttagcaaggt atccgtccca tacgagcgga ttttccggaa aatatttaac aaggaactct
     3061 ccgatcggtt ttaatgcgac acccggaaca acgctgaaga acataacaag tccaagaagt
     3121 gtaaactgcg gaacgagcat ccagaaaggt gcctctttca catgccgatg gttatccttg
     3181 agctgaccga gaaatatcgc aaatatcagc cggaaacagt aaaggaaagc gacaagccct
     3241 gcaaaacttg cgactgctcc ctgaaaatac atttgtgaat ctatcattgc gttgtagctc
     3301 agccattttc cgccgaatcc agtcaacggc ggcacaccgg aaagggcgat tatgccgatc
     3361 atcacagaaa gaaaacttat tggcattctt gttataagtc cgcccatttc atacatattt
     3421 cttgttttta ctctcatcac gactccaccg atcgcaagga acagcagtgc cttgaaaaca
     3481 aagtggttga gagcaaaagt ggttgcggta agccagccca gatggttcat catggaaaga
     3541 gcgaaaacaa ctccgccaag ctgaccgaca cttgaatagg caagaagccg ttttgcatct
     3601 tcctcaaatg ccgccatcat gttgccaaga agtgcggtta tgactccaag ccaaccgaga
     3661 acataataaa tatcaattcc tttaaagttc tgcggaccca ttgcaagcat tgtaatgatc
     3721 agtccgaata cgcccgcttt gagcagaacg gctgaaacaa caggcgacac atcgctttct
     3781 gcttcactgt gagctcccgg aagccagatg tgaaggggag ccgcggcagt tttagtgaga
     3841 aatcctatca caaagagaat aaatatcact gactgatatg ctccggcagt tgaaagtgtc
     3901 gcaagttcaa gatttccgtt atttgccgca ctgataagtc cgaatcctgc aagcattgaa
     3961 taggcgccgc caagagagaa tgtcatatag ctcaaagcgt gagccataga cttctttcct
     4021 cttaaaatga gaaaataact tccaagagtc atcagttccc accagaagaa gaattccatt
     4081 gtggtatcgg ctgtgaggag tcccgcaagc cctgcataca tcaaagcact tgacggatag
     4141 aatccgactc tttttccgct cttatcaaag ccggcaagaa gaaggatcat tccgccgata
     4201 agaaatacta tcagaaatac cgctctgaat tcaaaaagta ccggataaag gtgatagaaa
     4261 taatatccaa gccctgaaat gagcagaatg ttcttaattt ttgcagggag aaaatcaagt
     4321 atgaaaaatg atgtcgcaaa tatcagaggt attgaaactg ttttaagtaa cggcatgtga
     4381 ccgactgcaa aagggatgtc aattgagatt atgaaaagta ttgcaacaga tacaaaaagc
     4441 attgccaact ctttgaattt tatctctttt tgagtgtcgc tttccatttt tactgaatat
     4501 ccgaaccatc ttataagata gaccgcttca aggagagatc cgataagaat taacgccatc
     4561 cagagccagt ttgaagttcc ggcaagctgt tctatcagca tccattttgc aaaaaatgtc
     4621 gggaacgggg gaagtccgag aagagaaaaa atgaaaagcc cgagcagaaa tatgacaaac
     4681 ggattgcctt ttaattttcc ccactctttt gttttctcaa aacctgctat tccgttcagc
     4741 cagaaaaggc ctgtctttga aacggcatga accgcaacta tcgttcctgc gatgaaaaat
     4801 gtgtcagttc cgagaatatt gcctagaccg ataacggcag ttgcaagtcc accctgggca
     4861 attgatgata acccgagcat tttatttgtg ttttcggtct taattgctag cagatttgac
     4921 atcacgaaag tgagaagtcc ggtcactgcc accgcagaat tccagcttgg accgagaacg
     4981 ggaagaaatt tgtaaagagc gaaaagagaa gcagtcattc ctgctcccga tattgcggca
     5041 gttatgccgg caggtgcaga tttatagatg tcaaccgccc acctgttggc aggaaagggc
     5101 ttaagctcaa aaataagtgc aatgaaaaga aaaaacgcag cgatcgagcc ggtgccgagt
     5161 cctgaactgt tcattaaaac aattgtgtcg atatcaagaa caccggtctt tacatatgcg
     5221 gttatcgctc caagaattag aaaaattgat ataattcctg ttgccacaag ccacgaaaag
     5281 cccgctttga gaagttttcc cgacttttgg aatagaagca ttccggcaac tgcgatcgaa
     5341 cttatctcta tgaaaacaaa atagttgaaa aggtcccgtg tcattactat tccgtttaaa
     5401 gccatgaaat aaagaaggta tgtgatagct gaatatttgc cgttttcctt aatcaactta
     5461 aatatatgcg gcattccaag aagagcggtc agatttatca ccaacagtgc aaaagactcc
     5521 tcgatcccga ctttgagagc aattgaaaga ggggctgaaa atccggcagt tgattcgatc
     5581 atggagccgt ctttaaaagc aatgaagcgg aacgcccagt aagcggatat ggcagtaatt
     5641 gacatgatcg ctgtaaatac cataacaaac ggcagttttt cacttttttt gcttaatatt
     5701 gcagttaaaa aagcggttcc caatgataaa attatgatgt aaataggtgt tatcatttgg
     5761 cttctccgat tctgctgatt tccaaagttc cgtatttctc atacagtcgc cagacaaaga
     5821 caagcattat cgcagttatt ccgaaaccga tgacgatagc cgtgagcacc aacgcctgag
     5881 gaacagggtc aacgaccagc gtttccggat ttgccgcaag taaagcattg tcaaggatgg
     5941 gagctgttct ccctttaaga taaccgagag cgacgatgat tatgtggact cctgtatctg
     6001 caagtgaaaa tccgatcacc attttcatca ggttgttctt tgagaggatc gcataaaaac
     6061 cgatgaggat tagcagagag cccgtaatta ttcctgttac tgccatccta cacctccgaa
     6121 gcgttcttaa gttttacaag catggtcgaa agctctgagc cgacctttaa accgataagt
     6181 gtggatatca gagggatcgc tccggcagaa agaagccgcc ctgttattcc cgctccaagt
     6241 acagtcgcat caaggaatcc gccagcaagg ataagtccgg caacaccggt tccaacatag
     6301 aaaatgcctg aaaccgattc aagaactgta aaaagagtgg gggatccgtc agttttgttg
     6361 tcagcaagga tcaacaagag aagtgcagac gccatgacag ctccgccctg aaatccgccg
     6421 cccggagtaa gatgaccgtt cattacgata taaagccctg tcatgaaaat aaacggaaat
     6481 atcacttttg aagcggttgt aaggagttcg cttgattcgc tttttgtttc gtgacttctg
     6541 tttttaagaa aaaatgaaag aattgaagca gttaaaaaaa gcactgtcac ttcgcccaga
     6601 gtatcaagtc cgcggtaagt tacaacaaca cttgtcacga tgttgactcc gttaagttcg
     6661 gcaagtcctt ttgaaacata gtggcttgcg gcgcttccga gtgcaacggg atcggaatat
     6721 ccaagagcca gagcggcgaa cattgatgca acaccggcaa gaatgaaaat tatgaataag
     6781 tttctaacca ttttcatccc tcctcattct gcttaaaata agaaagaaca cgaccgtcat
     6841 cagcccgctt cctatcgccg cttcagtaag agcaacatcg ggagcgtcat aaatgatata
     6901 catgacactc gcaaaaagcc ctactgctcc gcttgcgatc acggcacttg tcagcgtttt
     6961 cactattacg gcaaaaacgg ctgagaagat gactacaagg gaaagaacaa ttaaaattac
     7021 cagttccatt tattccccct tatttatttt aaggttttta agatcatccc ggttgagttt
     7081 tcccttttca cagttgctgt ggtatccggc tcttccgatg cagtggctgg aaaccggatt
     7141 tgtaatgaca ataaatataa atattatcaa taatttaggc caccagacag gatttaagac
     7201 cgcaacacct gcaaggataa gtaaagaacc gagagtggag gcctttgttc ccgcctgaat
     7261 gcggttgaag aaatcgggca ttcttatcag tccgatgctt gctgtaaaaa ggaagaatga
     7321 tccggtaaga
//
LOCUS       DAOOYB010000139         7191 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_11775_length_7191_cov_12.175729, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000139 DAOOYB010000000
VERSION     DAOOYB010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7191
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11775_length_7191_cov_12.175729"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            61..1158
                     /gene="thiH"
                     /locus_tag="PL056_08780"
     CDS             61..1158
                     /gene="thiH"
                     /locus_tag="PL056_08780"
                     /inference="COORDINATES: protein motif:HMM:TIGR02351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HPM47150.1"
                     /translation="MFRPDDKEISEILKSSKKVTKSDILKAIYSETLSLNDLAALLSP
                     EIDNELLKKLAARAMELSRKRFGRAISLYIPLYYDNRCTNSCIYCGFNAKNQIERKKL
                     SFDEIMKEAEFIRSRNFKNILLVAGEHPESADVETLVPVIKKLHEIGFTCVSIEIAPL
                     EEDAYRTLVTDAHLDGLYVYQETYNKDIYSTVHPAGRKRDYDWRLETPERGANAGISK
                     IGIGFLTGLNDLSEDAMMLASHLIYLQKYHWQSEYSISFPRIRNAQGVNSEFKNISDR
                     QFIQLVCALRLMFPETGMSLSTREYPGFRDQIIPLGFTNLSAGSSTAPGGYCSESKDL
                     EQFEVEDSRTPEEIAEILKQRGFEPVWKDWQ"
     gene            1173..1817
                     /locus_tag="PL056_08785"
     CDS             1173..1817
                     /locus_tag="PL056_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008489551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HPM47151.1"
                     /translation="MEKKPIISEYTLGEEISHSITHGIGAALSIAGLVISVVMAARTG
                     EAIKVVSFAIFGTSMTILYTASTLYHAFTHEKVKNLFRYLDHTSIFLLIAGTYTPIAL
                     ILLKGAWGWTLFGLAWGLAILGILYQMLFLNKYKWISISIYLGMGWVSVIAIEPLIEL
                     MPFGLFAWILGGGLFYTLGTIFYLKEIRYFHFIWHLFVIAGTVCHFFGLIFYLV"
     gene            complement(1833..2864)
                     /locus_tag="PL056_08790"
     CDS             complement(1833..2864)
                     /locus_tag="PL056_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006869649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HPM47152.1"
                     /translation="MMKKAENNPGRVQIRNSTAEFLVFTKQSGENTIEVRVEDETVWL
                     TQKLMSVLFEKGRSTVTEHLQKIFETGELIEESVCRNFRHTAEDGKEYVTKFYNLDAI
                     IAVGFRVNSERATQFRQWAINILRDFAIRGYVLDKERLKNGAFLNKEYFDELLAEIRE
                     IRASERQFYQKITDIYATALDYDVESETTRTFFATVQNKLHYAIHGQTAAELIVSRAN
                     AEKEHMGLNTWKNAPEGKIIKTDVVVAKNYLKEKELRSLDRFVTMYLDYAEDQAEMGI
                     PMTMKDWAEKLNAFLKFNRREVLDNPGKVTSEIAKAFAESEFEKYRLVQDRLFESDFD
                     KLIKKLENK"
     gene            complement(2897..4816)
                     /locus_tag="PL056_08795"
     CDS             complement(2897..4816)
                     /locus_tag="PL056_08795"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HPM47153.1"
                     /translation="MKLWNVFFIIAALIFLSCSKSGPDYCINAFDCPEGYECIQGLCV
                     EPTGTNDNIANNDNEQHDNAVIPDNSIVPDESVNDDVNEDEDVITDEESEIDNETGDE
                     DCSPHSCGLFCSKVVTNECNTNSDCRCEPVKHFDNLADPDIYRDSDELYFISGTGVDT
                     NSLVVSMSSDLVVFEEAFTYIPSDADENFDYCCIWAPEIYKNPEGFYTIYFSAKRVAQ
                     GEVCDCADVSNVTTFYAQYDETAVNFKTPALVDFGAGNPQSSVSPECVVDGCEKTIRI
                     DSALFNDSGTEWFFWTWFQEGNYISSIKMNEPANVINNFQPTLMEGNINEGVDVFKRD
                     GKYYLFFSVNPFDGDYAMKYVVAETMDELVRARIPRWFSTPVKDNGGIRIQTHGHSSV
                     VERYGDHYIFYHQGRFDTQGILTGRDTYKSRLIFRGDGSLQMLNTIDIGWSDAGENFQ
                     YMLDVKPRDGDWVSPCLGNAILSINKKHTFTGICSTNSNTIVPKGSIEKIRLFYSDNG
                     VWGEPNMVEVDYDGFSSDISINIPDKKFDHLKIRFAQQNTGDIYSIDIKVKDGGWLAP
                     CIGSLTLSKPLSLSGDFAYTFNGTCLADPNNGTVVPIENIESVRVCSDKDDTWMTPVC
                     FEKTFDGNSRYIEVW"
     gene            4970..>7191
                     /locus_tag="PL056_08800"
     CDS             4970..>7191
                     /locus_tag="PL056_08800"
                     /inference="COORDINATES: protein motif:HMM:NF018449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HPM47154.1"
                     /translation="MMFSLSLSAGTVSKTAQLPFVKNSGQHPYDVLFSVAVPGGTAFV
                     TTDSSVIYDVKGAVFAEKLSSNSFIPQGLDESTAKVNIYKETGTFTGNVTFSSIGIER
                     IAENITVSMKATASSFEKIFTVYPGGNVSDIEIQLSGVDGISVHERSLLVYTNSGIAN
                     FTAPLAWQEVGGVRKDVAVEYKTSGKSYGFVTGEYDRSLPLFIDPLLSGVMIGGNNYD
                     ETTAMTVADNGDIIIAGTTRSGNFPVTNSSTYNSTNNLPDCVILRYDPSGTVLKAATY
                     YGGSRSECSSTGTDDSWTNMEKTYFAGVAAHGNSVFLTSLTYSTDLPMAGETYDKVCA
                     ACFGSNISAVFVAKFDESLTLTASTFYGGSAVDRARAITVDSSGNIYIAGDSASTNLP
                     SAGTLSGGTNDAFVASFPNSLATLRAAKMFGGSGVDRVMAMTANSNGIYLTGLTSSTN
                     FPVTEGAHSIDYGGSGGVGDAFVAKLNSSDLSIDKATYLGKKDGQYTSYGYAVTTNSD
                     GKVFVGGSTYSEPGHQFPVTAGVYKTTADTNPGGQWRTEGYIAKFPSDLGPVEACTYL
                     GSDSNHDDVKGLAMTSDGNILAVGFTSSNNFPVTADVYDSTHGGGWDGFISILTPDLT
                     TLVASTYLGAAGEDRLFATAIDAFGNITAAGFTKSTAIAGVTGTDDTDSEALLIRIRP
                     DLKAADAGVVLEIVPTEIDFGSKDINTQTSYSYIKVWNKGLTELKLSSVDVADTTNFF
                     G"
BASE COUNT         2029 a         1600 c         1445 g         2117 t
ORIGIN      
        1 ttcgacttga cattccctcc tgccgtttta ttatgaaccg gtttgttttt ttgaggttgc
       61 atgttcagac ccgatgataa agaaatatct gaaatcctta aaagttccaa aaaggttaca
      121 aaatctgaca tcttgaaggc aatatattct gaaacccttt ccttaaatga tcttgcggca
      181 cttctttctc ctgaaattga caatgaactt ttaaaaaaac ttgcggccag agcaatggaa
      241 ctctcaagaa aaagattcgg tcgtgcaatt tcgctataca ttccgctata ctatgacaac
      301 aggtgtacaa acagctgtat ttactgcggt ttcaacgcta aaaaccagat tgaacggaaa
      361 aagctttcgt ttgatgaaat aatgaaggaa gcggagttta tcagaagcag aaactttaag
      421 aacatccttc ttgtggcagg agaacatccc gaaagtgccg atgttgaaac gcttgttcct
      481 gtcataaaaa agcttcacga aatcggattc acctgtgtga gcattgagat agcaccgctt
      541 gaagaagatg catacagaac tctggtgact gatgcccatc ttgacggact ttatgtctat
      601 caggagacat acaataaaga tatttattca actgttcatc cggcaggcag aaaacgggat
      661 tatgactggc ggcttgaaac tcctgagcgt ggtgcaaatg caggaatttc aaagatcggg
      721 atcggttttc tgacagggct caatgatcta tctgaagatg cgatgatgct tgcaagtcat
      781 ttaatttatt tacagaaata ccattggcag agtgaatact ccatttcatt tccgcgtata
      841 aggaacgctc agggtgtaaa cagtgaattt aaaaatatct ccgaccggca gttcattcag
      901 cttgtctgcg ctctgcggct tatgttcccc gaaaccggca tgtcgctttc aacaagggaa
      961 tatcccggtt tcagagatca gatcattccg ctcggtttca caaatctgag tgccggctct
     1021 tcaacagctc cgggtggata ctgctcagaa tcaaaggacc ttgaacagtt tgaagttgaa
     1081 gattcaagaa ctcctgaaga gatcgcagaa attctaaaac agcgcggttt tgagccggtt
     1141 tggaaagact ggcagtaaat taaggactta gcatggaaaa gaaaccaata ataagcgaat
     1201 acacgctcgg tgaagaaata tcccactcga taacccacgg aatcggggcg gctctttcga
     1261 tcgcaggact tgttatctca gttgttatgg ctgcccgaac aggggaagcc attaaagttg
     1321 taagttttgc aatattcggg acttcgatga caattctata caccgcttca acactttatc
     1381 acgcattcac tcacgaaaaa gttaagaacc tgttcagata tcttgaccac acatcaatat
     1441 tcttattaat tgcaggaact tatactccta ttgcacttat tcttttaaaa ggggcatggg
     1501 ggtggacact ttttggactt gcatggggac ttgcaatttt aggaattctt taccaaatgc
     1561 ttttcctcaa taaatataaa tggatatcaa tctcaatcta tcttggaatg ggatgggtct
     1621 cagtaattgc gatcgaaccg ctcattgaac tgatgccgtt tggactcttc gcgtggatcc
     1681 ttggcggcgg acttttctac acactcggaa ccatctttta tcttaaagag atcagatact
     1741 tccacttcat ctggcacctt tttgtaatcg caggaactgt gtgccatttt ttcggattga
     1801 tcttttatct ggtatgagca cagtgagtta atttatttgt tttccaactt ttttataagt
     1861 ttatcaaaat cagactcaaa aagacggtct tgtacgaggc ggtacttctc gaactcactt
     1921 tcagcgaatg cttttgcaat ttcagaggta actttgccgg gattgtcaag cacttccctt
     1981 ctgttgaatt ttaaaaaggc attgagtttt tcagcccagt ctttcatggt cattggaatt
     2041 cccatttccg cctgatcttc tgcataatca agatacatgg ttacaaatct gtcgagcgat
     2101 ctgagctctt tttccttcaa ataattctta gcaacaacaa catccgtttt tatgattttt
     2161 ccttccggag cgtttttcca agtgtttaac cccatatgct ctttttcggc attggcacga
     2221 ctgactatta attcagcagc agtttgtccg tgtattgcat aatggagttt gttctgcact
     2281 gtcgcaaaaa atgttctggt agtttcgctt tcaacatcat aatcaagagc tgtggcataa
     2341 atatcagtta ttttctgata aaactgccgt tcacttgctc tgatctcgcg aatttcggca
     2401 agaagttcat caaaatattc tttgttcagg aatgcaccgt ttttcaatct ttccttatca
     2461 agcacataac ctctgatcgc aaaatcccgc aaaatgttta tcgcccattg ccggaattgc
     2521 gttgctcttt cggaatttac tctgaaaccg acagctataa tagcatcaag attataaaat
     2581 tttgtcacat attctttccc atcttcggca gtgtgtcgga aattccgaca tactgattcc
     2641 tcaattaatt cacctgtttc aaatattttt tgaagatgtt ctgtaacagt gctccggcct
     2701 ttttcaaaca gaacactcat taatttctgt gtcagccata cagtttcatc ttcaacacga
     2761 acctcaattg tgttttcgcc tgactgtttg gtgaaaacga gaaattcagc agtactgttc
     2821 cgtatttgca ccctgcctgg attgttctct gcttttttca tcatgctaat cctctaatcc
     2881 taataatcgt gagttttcac cacacttcta tatatcttga atttccgtca aaggtttttt
     2941 caaaacagac gggggtcatc catgtgtcgt ctttgtcgga gcatactctg actgattcga
     3001 tgttttctat cgggacaaca gtgccgttat tgggatctgc aagacatgtg ccgttaaatg
     3061 tatatgcaaa atctccgctc aagctcagcg gttttgagag tgtcagcgat ccaatgcatg
     3121 gagcaagcca gccgccgtcc ttgactttga tatctatgct gtaaatatcc cctgtgttct
     3181 gctgggcaaa acggatcttg agatgatcaa attttttatc agggatattg atcgaaatat
     3241 cacttgaaaa gccgtcgtaa tcgacttcga ccatgttcgg ttcaccccag acaccgttat
     3301 cagaataaaa aagtcgtatt ttttctatcg accctttggg aacgatcgta tttgaatttg
     3361 tactgcagat ccctgtgaat gtgtgctttt tgttgatgga aaggatcgca ttgcctaaac
     3421 acggtgaaac ccagtcgccg tctctcggtt ttacatcaag catgtactga aaattctcac
     3481 cggcatcact ccagccgata tcaattgtat tcagcatctg aagcgatccg tcacctctga
     3541 aaataagccg gcttttgtat gtgtcacgac ctgtcagaat tccctgtgtg tcaaacctgc
     3601 cctgatgata aaaaatgtaa tgatctccgt atctttcaac aacagaagag tgaccgtggg
     3661 tctggattct gattcctccg ttatctttca ccggagttga aaaccatctc ggaatccttg
     3721 ctcttacaag ttcatccatt gtttcagcga caacatattt cattgcataa tcgccatcaa
     3781 aagggttgac actgaaaaag agatagtact tgccatccct cttgaaaaca tcaacaccct
     3841 cgtttatgtt cccttccatc agtgtcggct ggaagttatt gataacattg gccggctcgt
     3901 tcatttttat tgatgagata tagttccctt cctgaaacca tgtccagaaa aaccattcag
     3961 tccctgaatc attgaaaagg gcagagtcga tccttattgt tttttcgcaa ccgtccacaa
     4021 cacattccgg acttacactt gactgagggt tgcctgcacc aaaatcaaca agggccggag
     4081 ttttgaaatt tactgccgtt tcatcatact gagcataaaa agttgtcaca tttgaaacat
     4141 cggcacaatc acaaacctca ccctgagcaa ccctttttgc agaaaaataa atggtgtaaa
     4201 aaccttccgg atttttataa atttcaggag cccagataca gcagtaatca aaattttcgt
     4261 ctgcatcact gggaatgtat gtgaacgctt cctcaaacac cacaaggtca gaactcattg
     4321 aaactacaag tgaatttgta tcaacgcccg ttcctgaaat gaaataaagc tcgtcagaat
     4381 ccctgtagat atcgggatct gcgagattgt caaaatgttt caccggctcg caccggcagt
     4441 ccgaattggt gttgcattca tttgttacaa ctttactgca gaaaagaccg caactgtgag
     4501 gagaacaatc ctcgtcacct gtctcattat caatttcgct ctcttcatct gtgatcacat
     4561 cttcgtcttc attgacatcg tcatttacac tttcatcagg aacgatgctg ttgtcaggaa
     4621 tgaccgcgtt gtcatgttgt tcattatcgt tatttgcaat attgtcattt gttccggtcg
     4681 gctcaacaca caggccctgt atgcattcgt aaccttcagg acagtcaaaa gcgtttatgc
     4741 agtaatcggg accgcttttt gaacaactca aaaatatcaa tgccgcaata ataaaaaaaa
     4801 cattccatag tttcattttt ttctctcctt cacacattca cactttttga tttaaccctt
     4861 tttgtaattg atttgcaata atatttaaga agaatataat ttcaggtgtt aaaacttttt
     4921 gaaggagatt taatatgaat cactccgttt tttttcagtt gcagtattta tgatgttttc
     4981 actgtcactt tcggcaggaa cggtttcaaa aaccgcacaa ttaccttttg tcaaaaacag
     5041 cggtcagcac ccgtatgatg tcctgttctc tgtcgcagtg cccggcggaa cagcttttgt
     5101 tactaccgat tcttcagtca tatacgatgt gaaaggggct gtttttgccg aaaaattaag
     5161 ttcaaacagc ttcattccgc aaggtctgga tgagtcaact gccaaagtga acatttacaa
     5221 agaaaccgga actttcaccg gaaatgttac tttttcttcg atcgggatcg aacggatcgc
     5281 tgaaaatata actgtttcaa tgaaagcaac cgcatcaagt tttgaaaaaa tattcacagt
     5341 ataccccggc ggaaatgttt ctgacattga aatacagtta agcggagttg acgggatttc
     5401 agttcatgaa agatctcttc ttgtctacac aaattcaggt atcgcaaact tcacagcacc
     5461 tcttgcctgg caggaggtcg gcggagtaag aaaagatgtc gcagtagaat ataaaacaag
     5521 tggaaaaagc tatggttttg ttaccggaga atatgacaga tcacttcccc ttttcatcga
     5581 tcctctttta agcggagtca tgatcggcgg caacaactat gatgaaacca ctgcaatgac
     5641 agtggcagac aatggagata taattattgc aggaacaacc cgctcaggca attttcctgt
     5701 aacaaattca agcacatata attcaacaaa caaccttccc gactgtgtca tcctgcgcta
     5761 cgacccgtca ggaacagttc tcaaggcggc aacatattac ggcggttcaa gatcagaatg
     5821 ctcttcaacc ggaacagatg attcatggac aaatatggaa aaaacttatt ttgcaggcgt
     5881 tgcagctcac ggaaattcgg tgtttctcac aagcttgaca tattcaaccg accttccgat
     5941 ggcgggagaa acctatgaca aggtttgtgc cgcctgtttc ggaagcaaca tctctgcagt
     6001 ttttgttgca aaatttgatg aatcgctcac tctcacggca tcaactttct acggcggatc
     6061 ggcagttgac agagcaaggg ctattaccgt tgattcttct ggaaacatct acattgcagg
     6121 agacagtgcg agcacgaacc ttccatctgc aggaactctt tcaggcggaa cgaatgacgc
     6181 attcgtagct tcatttccaa acagtcttgc aacactcaga gcggcaaaga tgttcggagg
     6241 aagcggtgtc gacagagtaa tggcaatgac tgcaaattca aacggaatat atcttaccgg
     6301 actcacctct tctacaaatt ttcctgtcac agaaggggcg cacagcattg attacggcgg
     6361 atcaggcgga gtgggagatg ctttcgtcgc aaaactgaac tcttcagacc tttcaattga
     6421 taaagccaca taccttggca aaaaggatgg acagtacaca agttacggat atgcagtgac
     6481 cactaattct gacggcaaag tttttgtcgg cggatcgaca tacagcgagc cgggacacca
     6541 gttcccggta actgccggag tttataagac aactgccgac acaaatcccg gcggacagtg
     6601 gagaactgaa ggatacatag caaaattccc gtctgatctc ggacctgttg aagcatgcac
     6661 atatcttgga agcgacagca atcacgacga tgtgaaagga cttgcaatga cttctgacgg
     6721 caacattctt gctgtcggat ttacttcttc aaataatttt cctgttactg ccgatgtcta
     6781 tgattcaact catggcggcg gctgggacgg attcatttca attcttactc cagaccttac
     6841 aacccttgtc gcttcgacat atctcggtgc ggcaggtgaa gaccggcttt ttgctacagc
     6901 cattgacgct ttcggcaata ttacagcggc aggtttcaca aaatcgactg caatagcggg
     6961 cgtaacagga actgacgata ccgattcaga ggcacttctg ataaggataa gacctgacct
     7021 taaagctgct gatgccggag ttgtgcttga aatagttccg actgagatcg atttcggttc
     7081 aaaagatatt aacacccaga cctcttacag ctatatcaag gtctggaaca aaggtctgac
     7141 cgagcttaaa cttagctccg tagatgttgc cgacactaca aactttttcg g
//
LOCUS       DAOOYB010000140         7150 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_11875_length_7150_cov_7.924172, whole genome shotgun sequence.
ACCESSION   DAOOYB010000140 DAOOYB010000000
VERSION     DAOOYB010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7150
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11875_length_7150_cov_7.924172"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1298
                     /locus_tag="PL056_08805"
     CDS             <1..1298
                     /locus_tag="PL056_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47155.1"
                     /translation="DQYNNTNIKGMYVSELITTAIPYSGIFVFVKDTVEVNAFAIGDK
                     ISVKGLYKEYYENSQVEVQSDDLSKLGTANVPNPAEIDDPSTVATPFEQSNDGWLPKG
                     IHGADGEKYEGVFIKVSDVEITNTNLGYGAWEITGNLAVDKEMFYWEGKREIGKVFTY
                     IQGILTFNYDAFKLAPRMESDLVEDSGDTGDTGDSGNTGDSGDTGDSGGCISECINLG
                     DKNCSDDFLQVVECIKNIDGCNYWVEIESCGENASCSNETTPAQCISDVVYSPVRDIV
                     QDSANTSSIKKNVFKGNEFTCNYNATIQNVEMYLDPSWDAEGDTTITFAIYEKNNTNW
                     TKIFDKQISTSNLNVGEQFFLTGYISVEIVKGKNYAIGAMFDRDCKYFSGNTTGQEVN
                     FGLQVGMVSKENLDGLPTILDEIYGYTIKMAITSKLINN"
     gene            1574..3127
                     /locus_tag="PL056_08810"
     CDS             1574..3127
                     /locus_tag="PL056_08810"
                     /inference="COORDINATES: protein motif:HMM:NF021349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM47156.1"
                     /translation="MRKKLSTSIYTFRTIKDQNCMYVDKTREMYDLVSRPNGQFFLSR
                     PRRFGKSLTLSTLESIFFGEKELFKGLYIYDQEYDWKKYPVIRLALNQLSSSTAEELE
                     ENLALAIKNVAGKYGIVLETERSYQRFKELIQELHDNHAPVVILIDEYDKPILDNILE
                     KEDVVKIRTLLKKFYGIIKASEEYLRFTFITGVSKFTQVSIFSDLNNLTDITLNPQFA
                     TICGFTQVECEHYFAEWIDENAVKNNMTKDQYLAKIKETYDGIRFTKKEAPVYNPVSF
                     TRAMDEGSFSHYWFETGTPTFLLKLLQEKEYEIVDFEELKLKENMFSSYEIDKLRVEP
                     LLFQTGYLTIKDYDPESDLYTLSYPNKEVRQSFVERLVDYFTPIAKEATPSLIEDLRA
                     AFLNDDMDEVFYVLKVFYSKIDNTIKIKQEKYYQTVFYILFTLLGYRIKAEVNTNKGR
                     MDAVIKTEKIIYIFEFKLNKTAKEAIEQIKQREYYQKYLLEKKTIKLIGIQFNHETGE
                     VKEWEVEKI"
     gene            3149..4564
                     /gene="pyk"
                     /locus_tag="PL056_08815"
     CDS             3149..4564
                     /gene="pyk"
                     /locus_tag="PL056_08815"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004491.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HPM47157.1"
                     /translation="MANTKIIATLGPASKDQGLIRDLILAGVDVFRVNMSHGSYDLVK
                     EIIGNVRAVSCDMKVNIPVFMDLRGPKIRIGIMENDEAMLEKGGICTINADPKTPGNA
                     SVFGTDYKYILTDVDKGDAVLLDDGKIVLEVESVDLKGVKCIVKTGGLLKSRKGLMLP
                     GKEIKLPSLSDKDIADVKYGMELGFDGFMLSFVQKSQDVIELQGIMQSYGKILPVISK
                     IESIKGLENLYSIAEVSDALLVARGDLGVELPVWELPFAQSQIIKAAHAHRKPVIVAT
                     EMLESMITNSRPTRAEVSDVAGAVTGGADLVMLSGETAVGKYPVLAVEFMDRTLEHAE
                     KMLAKPERVIDLNSDHIMSESVACSAVQLSKNIKAAAILIYTKKGFSARMISYFAPLC
                     PIYVFSEDPATARMCSIYRSTLPFVLSNKPENFEQFMTDSVEFLIDKNLLKKGDPVVA
                     VNVAGHSCSITHQNSIKVFAC"
     gene            4669..7056
                     /locus_tag="PL056_08820"
     CDS             4669..7056
                     /locus_tag="PL056_08820"
                     /inference="COORDINATES: protein motif:HMM:NF023459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HPM47158.1"
                     /translation="MKRLLILLAVLILAVSCGDLLTEKDKTDESGKQTENYGDNYGDD
                     VIGYRNHGKDSSSEEEDPNAGYQDSGGNGDTGDSGDTGDSGEYPDESTVADNEEMPDD
                     DNGISSSSVAKDSKWSDWIGIYVSGKLNDVNPQFVSEELFINGELNGDQLFGENNCQG
                     YMIKDGNNFVSTLAALVKSENNFEEYLHYYSFMPFENIKAAIVQGGVNEGYPAFVFST
                     VEENEKLRRECVLGVTMECAESGTCTSQFSGNTQFEADDYELYPGDDLKFNMNVELTT
                     NETKLIEAANSVRDIGEPDFVEPCLCYDSEGSVMDCTAFDEELGKRNLVLLNEENKVF
                     ENPFFTSTDRPAISFGLDVDSASYTVIRASLNNGSLPDPESVRIEEMINYFKYHYKKP
                     DPDQPFTLYSELGLCPWNLKRKMIMLGVRGSEIEFADVPPANIVYLLDVSGSMYDDIE
                     LVKKTFKMLVPQMREQDVVSIVTYAGAERVIIDGITGDQKETLIEAIDNLQSGGSTNG
                     AGGIIKAYELAEKHFVEGGNNRVVLATDGDFNVGLSSNEELVALIEEKKETGVFLSVY
                     GFGYGNYQDEKMEELTNAGNGTYFYIDGPEEAKRALANAFTGSMLAIAKDVKLQIQFN
                     PKHVKGFRLIGYDNRVMSNDEFNDDTVSGGVLGNGQDMTAFLEIIPADSTEDVPPIPE
                     GEDVNENDETEFEPLADDVFVVVRIRYKDPDSDESMLIENVLKKDDIRQIPSVKYYFA
                     AAVAELGLLLRHSVYFEERSIEKIIENVKIALQNDPEGAVKDFLEMVEKAHGLNP"
BASE COUNT         2369 a         1166 c         1610 g         2005 t
ORIGIN      
        1 acgatcagta caacaacaca aacattaagg gaatgtatgt ttctgagctt ataacaacgg
       61 caataccata ttcaggaata tttgtttttg ttaaagatac tgtcgaagtt aatgcttttg
      121 caatcggcga caagatctcc gtgaagggac tttacaaaga atactatgaa aattctcagg
      181 tcgaagtcca gagcgacgat ctttcaaaac ttggtacggc aaatgttccc aaccctgctg
      241 aaatcgatga tccttcaact gttgcgactc cttttgagca gagcaatgat ggatggcttc
      301 caaaaggcat tcatggtgct gacggtgaaa aatatgaagg tgttttcatt aaagtttcag
      361 atgtggagat caccaatacg aatctcggtt acggtgcatg ggagataact ggaaatctgg
      421 cagtagacaa ggaaatgttc tactgggaag gcaagagaga gatcggaaaa gttttcactt
      481 atattcaggg aattcttaca tttaattatg atgcattcaa acttgcaccg agaatggaaa
      541 gtgacctcgt tgaagattcc ggtgatactg gtgacacagg agattcgggg aatacaggtg
      601 atagtggaga tacgggtgat agtggtggat gcataagcga atgtataaat ctcggagata
      661 aaaattgttc tgatgatttt ttacaggttg tagaatgcat aaaaaatata gatggatgca
      721 actattgggt tgaaatagaa agttgtggag aaaatgcaag ctgttcaaat gaaacgactc
      781 cagcacaatg tatttctgat gttgtgtatt ctcctgttcg cgacatagtt caggatagtg
      841 caaatacaag ttcgataaag aaaaatgttt tcaaaggaaa tgagttcaca tgtaattata
      901 atgcgactat tcagaatgtt gaaatgtatc ttgatccatc atgggatgcg gagggggaca
      961 cgacaataac ttttgccatt tatgaaaaaa acaacacaaa ctggacaaaa atatttgata
     1021 aacaaatatc aacatcaaat cttaatgttg gcgaacagtt ttttctaact ggatatattt
     1081 ccgttgaaat tgtcaaagga aaaaattatg ccatcggagc aatgtttgac cgtgattgta
     1141 aatatttttc tggaaacaca acaggtcaag aagttaattt tggtttacaa gtgggcatgg
     1201 ttagcaagga gaaccttgat gggctaccga ctatacttga tgaaatatat ggatatacca
     1261 taaaaatggc tataacttct aagcttataa ataattaacc actggaaaca caggataata
     1321 aattcagaga aatttacaag cagtccacta aaagcacaca aaaactcagc ttttctgacc
     1381 gatttgtcgt tgctataaat ttaatgttca gcaaagttaa aatgtcttgt ttccggcaaa
     1441 gttaaaatgc tttgttcaaa agaattttct tgagcaacac ttttttatac agaattattc
     1501 atggattaat caataattga cacttcccgc tctattttta taatgttctc tgtccgttct
     1561 tatggaggga gtgatgcgaa agaaattaag cacgagtatt tacactttca gaacaataaa
     1621 agatcagaac tgtatgtatg ttgacaagac tcgtgagatg tatgatcttg tgagcagacc
     1681 gaatgggcag tttttccttt ccagaccgag aagatttgga aaatcgctta cactttcaac
     1741 gcttgaatca atttttttcg gagaaaaaga acttttcaag ggattataca tttatgatca
     1801 ggagtatgac tggaaaaaat atccggtgat acggctcgct ctcaatcagc tcagcagttc
     1861 aacggcagag gaacttgagg aaaatcttgc attagctata aaaaatgtgg caggaaaata
     1921 tggcatagtg cttgaaacag aaaggtcata tcaaagattt aaggaattaa ttcaggaact
     1981 tcatgataac catgctcccg ttgtaatcct cattgacgaa tacgacaagc cgattcttga
     2041 taacattctt gaaaaagaag atgtggttaa aatccgtact cttttaaaaa agttttatgg
     2101 aataatcaag gcatcagaag agtatttgag gtttacattc atcacgggag ttagcaaatt
     2161 cactcaggtt tccatatttt cagacctcaa caatctgaca gacataacct taaatcctca
     2221 atttgccaca atctgcggat ttactcaagt cgaatgtgag cattattttg cagagtggat
     2281 agatgaaaac gcagttaaaa ataacatgac aaaagatcag tatcttgcaa aaattaaaga
     2341 aacttatgac ggaataaggt tcacaaaaaa agaagcacct gtttataatc cggtatcgtt
     2401 taccagagca atggatgaag ggagtttcag tcactactgg tttgagacag gaacgccgac
     2461 atttctctta aaacttttac aggaaaagga atatgagatt gttgattttg aagagttgaa
     2521 gctgaaagaa aacatgtttt caagttatga aattgataaa ctccgtgttg agccgctgct
     2581 ttttcagacc ggttacttaa ccatcaaaga ttatgacccg gaaagtgatc tttatacact
     2641 gagttatcca aataaagaag taagacaatc gtttgttgaa aggctggttg actactttac
     2701 tccgattgca aaagaagcaa caccttcgct catcgaagac ttgagagcgg catttttaaa
     2761 tgatgacatg gatgaagtgt tttatgttct taaagttttc tattcaaaaa tagataacac
     2821 aataaaaatt aagcaggaaa aatattatca gacagtgttt tacatccttt ttactttact
     2881 tggttatcgc atcaaagctg aagtgaacac taacaaaggg agaatggatg cagtaataaa
     2941 aacggaaaaa atcatctata tttttgaatt taagctcaac aaaacagcaa aagaggcgat
     3001 tgagcagata aaacaacggg aatattatca gaaatacctt cttgagaaaa aaaccatcaa
     3061 actcatcgga atccagttca atcacgaaac cggcgaagtc aaagaatggg aagttgaaaa
     3121 aatctaaaat ggatattgga gttaattcat ggcgaacaca aagatcattg ctactttggg
     3181 tcctgcttcg aaggatcagg ggcttatcag agatctgatt ttagcgggtg ttgatgtttt
     3241 cagggtgaat atgtcccacg ggagttatga tctggtaaaa gagatcatcg gaaatgtgag
     3301 ggctgtttct tgtgatatga aggtaaatat tcctgttttc atggacttgc gtgggccgaa
     3361 gataagaata gggataatgg agaatgatga ggcgatgctt gaaaaagggg gtatctgcac
     3421 aattaatgct gatcccaaaa ctccgggtaa tgcttcagtt ttcggtactg attacaaata
     3481 tattctcact gatgtggata aaggggatgc tgttctgctt gatgacggga agatcgttct
     3541 tgaagtggaa tcggttgatt taaaaggggt taaatgtata gtgaaaacag gcggattgct
     3601 taaatcgaga aagggtttga tgcttccggg caaagagatt aaacttcctt ccttaagcga
     3661 taaggacatt gcagatgtga aatatggaat ggagctcgga tttgacggtt ttatgctgag
     3721 ttttgttcag aaaagtcaag atgttataga acttcaggga ataatgcaga gctatggaaa
     3781 aatactgcct gtgatttcaa aaatagagtc aattaaagga cttgaaaatc tttattcgat
     3841 tgctgaagtg agtgatgcac tgcttgttgc aaggggtgat ctcggtgttg aactgcctgt
     3901 ctgggaactt ccttttgccc agtctcagat aatcaaagcg gctcatgcac acagaaaacc
     3961 ggtcattgtg gcaacggaaa tgcttgaatc gatgataaca aattcccgcc cgacaagagc
     4021 tgaagtgagc gatgtcgcag gagcagttac aggcggagct gaccttgtca tgctttcagg
     4081 agaaacggct gtaggaaaat atccggtctt agccgtcgaa tttatggaca gaactttgga
     4141 acatgctgaa aaaatgctgg ctaaacccga aagagttata gatcttaata gtgatcacat
     4201 catgagtgaa agtgttgcat gttcagcagt tcagctttca aaaaatatta aagccgcagc
     4261 tattcttatt tatacaaaaa aaggattcag cgccagaatg ataagttatt ttgcaccttt
     4321 atgtccaatt tatgtctttt ccgaagaccc tgcaacagcg agaatgtgca gtatttacag
     4381 aagcactctg ccgtttgtac tttctaataa gcctgaaaac tttgagcagt tcatgacaga
     4441 ttcggtagaa tttctgattg ataagaacct tcttaaaaaa ggtgatcctg tcgtagctgt
     4501 aaatgtggcg ggtcatagtt gcagtattac gcatcagaat tcaataaaag tgtttgcctg
     4561 ttgattactt aatcggatat ttgaaaaaaa taaaatttct taaataaaaa tcgttatctt
     4621 ttaacacaaa accattgcgt tttaaataat tgttcaagga gagatgcgat gaaaaggtta
     4681 ttaattttat tggctgtttt gattttagct gtttcctgcg gagatctttt gactgaaaag
     4741 gataaaacag atgaatcggg gaaacaaact gaaaactatg gtgacaatta cggtgatgat
     4801 gtcatcggct acaggaatca cggcaaagac agctcttcag aagaagaaga tccaaatgcc
     4861 ggctatcagg actcgggtgg taacggcgat acaggagata gcggtgatac aggggattct
     4921 ggtgaatatc cggacgaaag cactgtcgct gataatgagg agatgcctga tgatgataac
     4981 ggaatctcaa gttcatcggt tgcaaaggat tcaaagtgga gtgactggat cggaatatat
     5041 gtcagcggaa aacttaatga tgtgaaccct cagtttgttt cagaagaatt gttcataaac
     5101 ggagaattaa acggtgatca gctttttgga gagaataact gtcagggata tatgatcaag
     5161 gacggcaata atttcgtttc aacgcttgct gctctggtaa aaagcgaaaa caattttgaa
     5221 gagtaccttc attattattc atttatgcca tttgaaaaca tcaaagcagc aattgttcag
     5281 ggtggagtta atgagggtta ccctgctttt gttttcagta cagttgaaga gaatgaaaaa
     5341 ttaaggagag agtgtgttct cggagtcaca atggagtgcg ctgaaagcgg aacatgtaca
     5401 agtcagtttt cggggaacac tcagtttgag gcagatgatt acgaattata tccgggtgac
     5461 gatctgaaat tcaacatgaa tgtggagctt acaacgaatg agacaaaact catcgaggcg
     5521 gcaaattctg tccgtgacat aggtgaaccc gattttgtgg agccgtgtct ttgttatgac
     5581 agcgaaggca gcgtcatgga ctgcactgca tttgacgaag agctcggaaa gagaaatctc
     5641 gtcctcttaa atgaagagaa taaggttttt gaaaatcctt tttttacatc aaccgataga
     5701 cccgcaatta gtttcgggct tgatgttgac agtgcttcat acacagtgat aagagcgtca
     5761 ctcaataacg gttctcttcc tgatccagag tcggttcgta tagaggaaat gatcaattat
     5821 ttcaagtatc attacaaaaa acctgaccct gatcagccgt ttacgcttta ttcggaactt
     5881 ggattatgtc cctggaatct taaacgaaaa atgataatgc tcggagtgag gggatcagag
     5941 attgaatttg cagatgttcc tccggcaaac attgtttatc ttcttgatgt gtcaggatca
     6001 atgtatgatg atatcgaact tgttaagaag acctttaaaa tgcttgttcc gcagatgaga
     6061 gagcaggatg tggtgtcgat cgtgacctat gccggagctg aaagagtgat aattgacgga
     6121 ataaccggag atcagaagga aacgcttatt gaagcgattg acaacttaca gtcaggcggt
     6181 tcgacaaatg gtgcaggcgg cataatcaag gcatatgagc ttgctgaaaa acattttgtt
     6241 gaaggcggca acaacagagt cgttcttgca actgacgggg attttaatgt ggggttaagc
     6301 agtaacgaag agcttgttgc tttaattgaa gagaaaaagg agacaggagt ttttctttca
     6361 gtttacggtt tcggatatgg aaactatcag gatgaaaaaa tggaagaact gacaaatgca
     6421 gggaatggca catattttta cattgatggc cccgaagagg caaagagggc gcttgccaat
     6481 gctttcaccg gttcaatgct tgcaattgca aaggatgtca aactgcagat acagttcaac
     6541 ccgaaacatg ttaaaggttt ccgcctgatc ggttatgaca acagagtcat gagcaatgat
     6601 gaattcaatg atgatacagt gagtggaggc gttctcggaa acggtcagga tatgacagca
     6661 ttccttgaaa taattcctgc cgattcaacc gaagatgttc cgccgattcc tgagggagag
     6721 gatgttaacg aaaatgatga aacagagttt gaaccccttg cggacgatgt tttcgtagtt
     6781 gtaagaatca gatacaaaga tcccgacagc gatgaaagca tgttgatcga aaatgttctt
     6841 aaaaaagatg acataagaca gatcccgtca gtgaaatatt actttgcggc ggcagttgct
     6901 gagcttgggc ttctgcttcg tcactcggta tattttgaag aaagatcaat tgaaaagatc
     6961 atcgaaaatg ttaaaattgc actccagaac gaccctgaag gagcagtaaa ggatttcctt
     7021 gaaatggtcg aaaaagcaca cggacttaat ccgtaaaaca tcacttacaa tttttaagaa
     7081 atttgttcct aaattatcct gtcaagacat ttaaaaacct taccagagag tcaatcgtcg
     7141 agacattaag
//
LOCUS       DAOOYB010000141         7146 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_11887_length_7146_cov_10.362572, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000141 DAOOYB010000000
VERSION     DAOOYB010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7146
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11887_length_7146_cov_10.362572"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            86..1966
                     /locus_tag="PL056_08825"
     CDS             86..1966
                     /locus_tag="PL056_08825"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HPM47159.1"
                     /translation="MKKIIISVLVLIMFFACTEKKVEKKEVLDSVTKTISASEGGEIA
                     TEDGEGKIEIPAGALEKDTEITMTRYKKDGFPEKDDLISKPFEFAPAGLKFKKDISVT
                     IKADTGLTKIPAGKRVAAGLLNDSGSDWLHEGSGDPIMSGDPIMSGDPIMSGDPIMSG
                     DPIMMTVGHFSTVVFIVVDVNEETSDLENVDSDVETADEASDEAADETDKENHADSDE
                     ITDEENDAVVENDDDIPDSEMVDNEMPDMDDVVDLCGNGMIDGSELCDNHMKKPCGII
                     DPLLYTGGDADCNSVCDGWDTAKCVERVPFAGVLCTGQTLCYDNSTTITCPSTDSESF
                     YGQDATYGTANCVAKNFMMTGAPVEEVIVDQNIGIMWQRNLPEIIPGCSMGEMGETCK
                     KDEAVSYCETLVYSGYDDWRLPTIEELETIIDYGKSEPCYDQALFVDNQNGLGTSLWS
                     GSFYLEETYTYHFFLSPFGNVYSEIIDSGEFDPSARARCVRGNIYAPVPSFSEETVGE
                     DELVKDARTGLWWMKNFYTYSEWMMGMSNCEASEAGGHTDWRMPNINELKTILDRSIN
                     NPPTTFPGVSTGYFFSSTTDSHVEGNGSAWVIDSQYGTSYSNNKTEHNDASTLCVRGP
                     VE"
     gene            complement(1972..2883)
                     /locus_tag="PL056_08830"
     CDS             complement(1972..2883)
                     /locus_tag="PL056_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47160.1"
                     /translation="MSEKTKTIFLYLFLFSVVIARIILETAVFSKRYFFSYFVTLHHF
                     CWYMFVFHYFALCSRYVLGMKRESVKYFALLSPVIFTPIVHSYFSGRKMNLEYMWGDL
                     SRVLFNIATLYKFHPKNSFFFIEMVLLLVIFIAGSWFISKSVRRTFLNIVIGFYGSMI
                     LAGLHLFGVYPKTKAYFKIHTSLKNHPLMTLIYFSLFILVFLIYSYPEIKKAFLRSKK
                     NYLIAFVIAISISIPSTLYGLVHFTGKSPQPADIFLIAVPFTALFIGIAAMIKKNSSG
                     FVAGRFFPFVFSVISVLILGGIILQIK"
     gene            complement(2876..3808)
                     /locus_tag="PL056_08835"
     CDS             complement(2876..3808)
                     /locus_tag="PL056_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013898914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HPM47161.1"
                     /translation="MEKNSLVYVAGHRGLAGSAIVNELKRSGYENLLLRTSGELDLRN
                     QHEVEKFFNEFKPEYVILAAARVGGIGANSRFRAQMIYDNLMIQSNVIHHAYLNNVKK
                     LLFLGSSCIYPKFAPQPMKEEDLLTSSLEPTNEPYAVAKIAGLKMCEAYNSQYGTDFI
                     TVMPTNLYGENDNFDLENGHVFAVMIRKIHEAKINSSEFVELWGTGNPRREFLYSGDL
                     AKAVVMLLNSADKFKTHTNIGSGRDITIRELAELIKDIVGYSGEIKWDSSQPDGTPQK
                     LLDITKISQTGWVPELSLSAGIKKVYEWYCENHE"
     gene            complement(3879..5945)
                     /locus_tag="PL056_08840"
     CDS             complement(3879..5945)
                     /locus_tag="PL056_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47162.1"
                     /translation="MNFIKYLSLIFVAALIISCGDDKKDPGDTGNTGNTGNTGDTGDT
                     LPDENGDTGNSGNTGNTGNTGNTGNTGDSGNTGDSGDTGDSGKCDCNGTEYLIPDQFK
                     GIEDWCMSDEDEDTIPNCIEVPNGIPVDTDDDTTADYLDTDSDGDGIPDSVECPELPC
                     RDTDDDGVPDYRDTDSDDDAILDSIECPEQPCVDTDDDKTPDYLDLDSDGDGIPDLYE
                     GTEDADEDKIPNYLDTDSDGDGISDEIENDGHIPPLDTDKDGTPDYLDLDSDNDGLSD
                     AKELEVGTDPKKKDTDGDGIDDATEYAAGSDPTVANAEWWKDKYYVILPYNDPDHEIR
                     PLDFSTDITKADILFLIDLSESMDGEIAHLKDGINNTLIGSIKAKIPDAGFGITTFED
                     YDTFPNDYGTRVIQTITTNENTLKNTMSTVNYLENTGWEPQMSAIYFSATGEARGTTP
                     AMDCSASEGSIGGACFRPGALPIFIMMTDEAFETGYSKYKVGASDWIYVSTNNAIDAL
                     NGINGKFIGVDSWNTGSFGFTPANDLKQVSTGTGSVDGSGNPFYYLINSNGTGLSTNI
                     ANAVEALATSVPMLVNTGRESITNPQSVDVTQFIKAVTPVKRVVGGNTVNCPAECTSV
                     AFENVKPGTTVTFDIDFYNDIFNPTTPEPTAFQAKIHVYGEGSLVDTREVYIIVPGKT
                     GTGPGS"
     gene            complement(5972..>7146)
                     /locus_tag="PL056_08845"
     CDS             complement(5972..>7146)
                     /locus_tag="PL056_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HPM47163.1"
                     /translation="DTDDDGFDDNTEYAAGSNPTVSDPEWWEGKYYVILPYNDPDHEI
                     RPLDFSTNLSKVDIMVIIDVSGSMQQEIDNLKTKLRTAVIPGINNIMPGVNDFAGFGL
                     MSFGNNKDLMRQAVTTNSSTIDTAINGLPAASGGAECHSEALYQAAIGDGNAGSGHFP
                     KPNCTGQEGNKGGGCYRDRALPIFLMLTDESFDDLDLVHSRTQAIAAMNSEWLKAKFI
                     GIATGTSNPNNDFKAVSQGTGSVDTNGNPFNYQIGTDAVGLDTKIIDAVTQLANNIQM
                     EITTTKSKVELPESFDDLVDSSNFIKSVKPKSLNSIPVINCPEASSCDNTKFFKVKAG
                     IGVTFEIDFYNDFYEPTTTETTVFRAKIDVLGEGAALDSREVYIIVPGKESTGPGN"
BASE COUNT         2040 a         1437 c         1544 g         2125 t
ORIGIN      
        1 cgatgttaag gaatggctca acttttgaca aaaaaaatct tttgatttaa tattcttaag
       61 tttttaattt tttcaggagg ggaaaatgaa aaaaattatt atttctgtgc tcgtactgat
      121 catgttcttt gcatgtacag aaaaaaaagt tgaaaaaaaa gaggttcttg attctgtaac
      181 aaaaacaata tcggcatctg aagggggaga gatcgcaaca gaagacgggg aaggtaagat
      241 cgaaattcct gccggtgctc ttgagaaaga tacagagata acaatgacca gatataaaaa
      301 ggatgggttc ccggaaaaag atgatcttat ttcaaaacct tttgagtttg ctcccgcagg
      361 acttaaattt aaaaaagata tttcagtcac catcaaagca gacaccggat taacaaagat
      421 ccctgccggg aagagggttg ctgccggatt gctgaatgat tccggctcag actggcttca
      481 tgaaggaagc ggcgatccga tcatgagcgg tgacccgatt atgagcggcg acccgataat
      541 gtcaggtgac ccgattatga gtggtgaccc gataatgatg acggtcggtc atttcagcac
      601 agttgtcttt attgttgttg atgttaatga agagacatct gatcttgaaa atgtggacag
      661 tgatgttgaa actgcagatg aagcatcaga tgaagcggca gatgaaactg acaaggaaaa
      721 tcatgctgat tctgatgaaa ttacagacga agaaaatgat gctgttgtgg aaaacgatga
      781 tgacattcct gacagtgaaa tggttgataa cgaaatgcca gacatggatg atgtcgtaga
      841 tctttgcgga aacggaatga tcgacgggag tgaactttgc gacaaccaca tgaaaaaacc
      901 gtgcggaatc attgatcctc ttctttatac aggcggtgat gctgactgca acagtgtctg
      961 tgacggatgg gatacagcta aatgtgttga aagagttcct ttcgctggag ttttgtgtac
     1021 agggcagacg ctctgctatg ataattcaac aacaattaca tgtccttcaa cagatagtga
     1081 atcattttac ggacaggatg caacatatgg aaccgccaac tgtgtggcta agaattttat
     1141 gatgacggga gctccggtag aggaagtgat agttgatcag aatatcggca tcatgtggca
     1201 gagaaatctt cccgaaataa ttccgggctg ctcgatgggt gaaatgggtg aaacatgcaa
     1261 gaaagatgaa gcagtcagtt attgtgaaac ccttgtatat tcagggtatg acgactggcg
     1321 actgccaacc atcgaagaac tggaaacaat aattgattac gggaaaagcg aaccttgtta
     1381 tgatcaagcg ctgttcgttg acaatcaaaa tggtctaggc acaagtctct ggtcaggttc
     1441 cttttacctg gaggaaacat atacctatca tttctttctt tcacctttcg gtaatgttta
     1501 tagcgaaatt attgattcag gagagtttga tccttcagcg agagcaagat gtgtcagagg
     1561 aaacatttat gcaccggttc cctctttttc agaagaaact gtcggagaag acgaactggt
     1621 aaaagatgcc agaacaggtc tttggtggat gaagaatttc tatacataca gcgaatggat
     1681 gatgggcatg agcaactgtg aagcaagtga ggccggagga cataccgact ggagaatgcc
     1741 taatatcaac gaactcaaaa ccatccttga cagaagcatc aataaccctc caaccacctt
     1801 tcccggtgtt tctacaggat atttcttttc ttcaacgaca gacagccatg ttgaaggaaa
     1861 tgggtcggca tgggtaatag atagtcaata cggaacatca tattccaaca ataaaacgga
     1921 acacaatgat gcaagcacac tgtgtgtaag aggtccggta gaatagatca ctcactttat
     1981 ctgcagaatt attccgccaa gtatcagaac cgaaataact gaaaatacaa acgggaaaaa
     2041 tcttcctgcg acaaatccgg atgaattttt ctttatcatt gcggcgatcc cgataaaaag
     2101 tgcggtgaag gggacggcaa tgagaaatat gtcggcgggc tgaggtgatt tccctgtgaa
     2161 atgaacaagt ccgtaaagtg ttgaagggat tgaaatcgat attgctataa caaaagcgat
     2221 aagataattt ttctttgatc ttaggaacgc ttttttaatt tccggatatg aatagataag
     2281 aaaaaccagt atgaaaagag aaaaatagat cagggtcata agcggatgat tttttaagga
     2341 ggtgtgtatt ttgaaatatg ccttggtttt cggataaacg ccgaaaagat gaagcccggc
     2401 aagtatcatt gagccgtaaa agccgatgac aatattaagg aatgttcttc tgacgctttt
     2461 tgatatgaac catgagccgg caataaagat aacaagaagc agtaccattt caatgaagaa
     2521 aaaactgttt ttgggatgaa atttataaag tgttgcgata ttaaaaagaa ctcttgaaag
     2581 atctccccac atatattcaa ggttcatttt ccgtcctgaa aagtatgaat ggacgatagg
     2641 ggtgaaaatg actggtgaaa gcagcgcaaa atattttacc gattcccttt tcattccgag
     2701 aacatatctg ctgcaaaggg caaaataatg aaaaacgaac atataccagc agaaatggtg
     2761 aagtgttaca aaataagaaa agaaataacg ctttgaaaat actgcggtct caagaatgat
     2821 ccttgcaatt acaaccgaga acagaaacag atagagaaaa atggtttttg ttttttcact
     2881 catggttttc gcagtaccat tcataaactt tttttattcc tgctgaaagc gaaagctcag
     2941 gaacccatcc ggtttgagat atttttgtta tatcaagaag tttctgcggt gttccgtcag
     3001 gttgagagct gtcccacttt atttctccgg aatagccgac gatatcttta attaattcag
     3061 caagctccct gatagttatg tctctgccgg agccaatgtt tgtgtgtgtt ttaaacttat
     3121 cagcagagtt taaaagcatg acaaccgctt ttgcaagatc tccgctgtaa aggaattccc
     3181 ttctcggatt tcccgttccc caaagttcaa caaattcaga actgttgatt ttagcttcat
     3241 gtatctttct tatcatcacc gcaaagacat ggccgttctc aaggtcgaaa ttatcattct
     3301 ccccataaag gtttgtcggc ataactgtaa taaaatcagt gccatattga gaattgtaag
     3361 cttcgcacat tttgagcccc gcaatttttg caacggcata tggctcattt gtcggttcaa
     3421 gtgatgatgt gagcagatct tcctctttca tcggttgagg ggcgaatttc ggataaatac
     3481 agctcgatcc gagaaacaga agctttttca cattgttaag atatgcgtga tggatgacat
     3541 tcgactggat cataaggttg tcatatatca tctgggcacg gaaacggctg ttggcaccga
     3601 ttcctcccac tcttgcagcg gcaagtatca catattcggg cttgaattca ttgaagaatt
     3661 tttcaacttc atgctgattg cgaagatcaa gttcgcctga agtcctaaga aggagattct
     3721 cgtaacccga tcgtttcagc tcgtttacga tcgcacttcc ggcaagccct ctgtgacctg
     3781 caacataaac aagagaattt ttttccataa ttcacaccga ataaacaaaa ataatggtga
     3841 tgttatcaga tgaacgggtt ttaggcaagt tgaatcagtt aacttccggg acctgttccg
     3901 gttttgccgg gaacgatgat gtaaacttca cgggtatcaa caagtgagcc ctctccgtaa
     3961 acatggatct ttgcctgaaa ggcggtcggt tcaggtgttg tgggattaaa aatatcgtta
     4021 taaaaatcaa tatcgaatgt tactgttgtt cccggtttga cattttcaaa agcaacggat
     4081 gtgcattctg ccggacagtt tacagtgttt ccgccgacaa ctcttttcac aggtgtaact
     4141 gcctttatga attgagtaac atctacagac tggggatttg ttattgattc tctgccggta
     4201 ttgacaagca tcggaacgct tgtcgcaaga gcttcaacag cgtttgcaat gttggttgaa
     4261 agaccggttc cgtttgaatt aataaggtag tagaagggat ttcctgaccc gtcaactgag
     4321 ccggttcctg ttgaaacctg tttcagatcg ttagcagggg taaaaccgaa agatcctgta
     4381 ttccatgagt caacaccgat gaatttgccg tttatgccgt ttaacgcatc aatcgcattg
     4441 tttgttgaaa catatatcca gtcgctcgca ccgactttgt atttgctgta tcctgtttca
     4501 aaagcttcgt ctgtcatcat tatgaatata ggtaatgccc ctggtctgaa acatgctccg
     4561 cctattgagc cctcacttgc tgagcagtcc attgcgggag ttgttccccg tgcttcacct
     4621 gtcgctgaaa aatagattgc gctcatttgc ggttcccagc cggtattttc aagatagttc
     4681 actgttgaca ttgtattttt taatgtgttt tcgttggtgg tgatagtctg aatgacccgg
     4741 gttccgtaat catttgggaa tgtatcataa tcttcaaaag ttgttattcc gaagccggca
     4801 tcgggaattt ttgccttgat cgatccgatg agtgtgttat ttattccgtc tttcaggtgt
     4861 gctatttctc catccattga ctctgaaagg tcaatgagaa aaaggatgtc ggcttttgtg
     4921 atatcagttg aaaagtcaag aggtctgatc tcgtggtcag gatcgttata tggaaggatt
     4981 acataatatt tgtctttcca ccattccgca tttgcaacag taggatcact tcctgcggca
     5041 tattcagtgg catcatcaat tccatctccg tcggtatctt ttttcttcgg gtcagttccg
     5101 acctcaagtt cctttgcatc actaagtccg tcattgtcgc tgtcaaggtc aagataatcg
     5161 ggagttccgt ctttgtctgt gtcaagcggg ggaatgtgcc cgtcattttc aatttcatcg
     5221 ctaatcccgt caccatcact gtcggtatca aggtaatttg gaattttatc ttcatcagca
     5281 tcttcagttc cttcataaag atcgggaatt ccatcaccgt cactgtcaag gtcaagatag
     5341 tcaggagtct tatcatcatc tgtatcaacg cagggctgct caggacattc aatactgtct
     5401 aaaatagcgt catcatcact atctgtatca cggtagtcgg gaacaccgtc atcgtctgta
     5461 tccctgcatg gaagttcggg gcattcaact gagtcgggaa ttccgtcacc gtcgctgtca
     5521 gtatcaagat aatcggcagt ggtgtcatca tcagtgtcaa cgggaattcc gtttggaacc
     5581 tctatacagt tggggattgt gtcttcatct tcatcactca tgcaccagtc ctcaatccct
     5641 ttaaattggt ccggaattaa atattctgtg ccgttacagt cacattttcc agagtctcct
     5701 gtatcacctg agtcgcctgt attgcctgag tcgcctgtat ttcctgtgtt accagtattc
     5761 cccgtatttc ctgtgttgcc tgaatttccc gtatcaccat tttcatccgg aagggtgtcg
     5821 ccggtatcgc ctgtgttacc tgtatttcct gtattacctg tgtcaccggg atcctttttg
     5881 tcatctccgc atgagataat caaagcggca acaaaaatta acgacagata cttaataaaa
     5941 ttcataagaa acctcttctt aaaaatatta tttaatttcc aggccctgta ctttccttgc
     6001 ccggcactat gatatagacc tctcttgaat caagagcggc tccttcaccc aaaacatcta
     6061 tctttgctct gaaaaccgtt gtttcagtag ttgtcggctc ataaaaatca ttataaaaat
     6121 ctatttcaaa agtaacgccg attccagctt tgactttaaa gaattttgta ttatcacagc
     6181 tacttgcttc aggacaattt atgacaggta ttgagttcaa cgatttgggt ttaactgatt
     6241 ttataaaatt cgaactgtct accagatcgt caaaagattc gggaagttca acctttgatt
     6301 ttgtagttgt aatctccatt tgaatattgt tggcaagctg tgtgacagca tcaattattt
     6361 tagtgtccag acctacggca tctgttccaa tctgataatt aaaaggattt ccgtttgtgt
     6421 caacagagcc agtaccttga gaaacagctt taaaatcatt gttggggtta gatgttccag
     6481 ttgctattcc tataaactta gctttaagcc attctgaatt cattgcggca attgcctgag
     6541 ttcttgaatg aacaagatct aaatcatcaa aagattcatc agtcagcata agaaaaatag
     6601 gcagtgccct atctctgtaa caaccaccgc ctttgtttcc ttcctgtccc gtacagttgg
     6661 gttttggaaa atgtccgctt cctgcatttc cgtctccgat cgctgcctga tataaagctt
     6721 cgctatgaca ttccgcacca cctgaagccg cgggtaaccc attaatagct gtatcaatag
     6781 tgctgctgtt tgttgttact gcctgtcgca ttaaatcttt gttgtttcca aatgacataa
     6841 gtccgaatcc tgcaaaatcg tttactccag gcatgatgtt attaatcccc ggaattacag
     6901 cggtgcgcag ttttgtttta agattgtcta tttcctgttg catcgagccg cttacatcaa
     6961 taatcaccat aatgtccact tttgataaat tagtgctgaa atccagaggt cttatttcgt
     7021 ggtcaggatc gttgtaaggc aggatgacat aatactttcc ttcccaccat tcagggtctg
     7081 aaaccgtggg gttgcttcct gccgcatatt ctgtgttgtc gtcaaaacca tcatcatcgg
     7141 tgtcgg
//
LOCUS       DAOOYB010000142         7130 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_11927_length_7130_cov_7.497951, whole genome shotgun sequence.
ACCESSION   DAOOYB010000142 DAOOYB010000000
VERSION     DAOOYB010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7130
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11927_length_7130_cov_7.497951"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="PL056_08850"
     CDS             <1..589
                     /locus_tag="PL056_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47164.1"
                     /translation="CSDEWMECKPEFGACGAKSGRCDTDADCSGLAPVCNTGTHLCES
                     PSSLLSNGSFETWTDPAYPDFWKGTKTDIAAVNIIHETVNVHSGSSALMLVNTSETHK
                     RFTSQSVALTAGSYTCKYFASGSGEIRNAFYSNGTGDYIYSSYTVLASVNWTEITYNF
                     TLGTDRAVFELIFSVRSTTDTSGHVKVDDVVCNKN"
     gene            668..1918
                     /locus_tag="PL056_08855"
     CDS             668..1918
                     /locus_tag="PL056_08855"
                     /inference="COORDINATES: protein motif:HMM:NF012693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HPM47165.1"
                     /translation="MKFLSFSLTDTGKKRRNNEDAFINMAQIGLFAVAVGMGGEQCGE
                     IASQTAVDSITHFINENHAIIGAFEMNDSAENKTQVLNMLADSLRFANMKVFREAEKR
                     NLQGKMGTTLTIFLAIDNAGFMVHAGDSRLYMIRGDEITQLSTDHTVSNDYKRQYGDA
                     GEGFDERFAGILTKAVGIQEYVEPEKLTFTIVPFDRYLLCSDGLYNAYGNERENAEKL
                     LMSREHDIEKESSFLSDAAGQLLDIAYENGASDNVSMVLVSAFSMDEEEVTGTKELIK
                     KFDIVRNIDLFRNLDFKELLTVMERVEIRTYNRYDVISRIESPDRELFIILEGKVSSL
                     RGSKHLKTFYEGDHIGEVAFLTEEKPQFNLFVDKASSFLVIKRSVFIQMMNDNPALGV
                     KLLWQLATVLARQTNSSIGLIKEE"
     gene            1915..4047
                     /locus_tag="PL056_08860"
     CDS             1915..4047
                     /locus_tag="PL056_08860"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HPM47166.1"
                     /translation="MITSQIIVTFFLTVSALQFDFEGGNIEDHINKKILYLQQFTDEE
                     RIGFCKAGDVLSCKILFLKEINDRNASSESIEGYLDLLPDEHRYSGIYLYLTGKWKDI
                     PIEKLKIYMGYLPEELSKGLYNIYLKKLYATGNIKAFMDDYIQGRNAELTSYYINSML
                     KKDPGEALEYLRSLKVSFPESFYDSISKDVEKYATKLKGDSYNEFRIWNLEFNYRKVR
                     YNQAISLASKWFPSKKYSNSYTWRAHLYRAMSHTKKREHDKARAVYGKLEEYLSNELL
                     SDGDIYKFFHEYGYSEAALGDNDKSIELYLKGYEYFISKDEESAAGFLYMAADMSRLA
                     QYWDKAEELYSLYLKNHPGSGRRNMVEFLLFWINYRQKDFITAKVLLNNILDGSPVMS
                     YDHQRASYWLARVTARLGDTVASISLLCSLSAKFPASFYGSMAASRIRENGLTCMENA
                     NEQNSSFEQFRFREEKMMPETGWLTAAMVTGERAVIKKMLNAVSKVINEKGDEIDRLT
                     ASYIAKTVDDHALAANLIKSISNFSGTSKEYFKLQYTIAFEEEILSHCDFYGVPPMFV
                     FSIARQESLFNTGAVSASYAIGLLQILPTTAQLLANREDYGKINPEVLKKPLTNVRFG
                     VRFLSDLLIKFNGSIPLAAASYNAGPGRIMKWMEKSPDMEIDEFIEDIPIFQTRNYVK
                     KVMSNYAVYTYIFEDRVYDGLEFKLPVE"
     gene            4078..5268
                     /locus_tag="PL056_08865"
     CDS             4078..5268
                     /locus_tag="PL056_08865"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HPM47167.1"
                     /translation="MKYSIIPAAGLATRFLPASKAIPKELFPVLDKPVIQYIVEEAVS
                     AGISAIVFVSGSGKEAILDHFDRLNPKFSVSKLNDEIKSKVDELDSMIDVFSVRQKDP
                     LGLGHAVLKGAYSIKDEPFAVLLPDMLIIPRKGVSSMKKMVELYEKTGLSVIALMKVP
                     QKMKGLYGVAEVKPTDDENVVEIGKLFEKPLQGETESDLALVGRYIFSPDIVNILKNT
                     KPGKGGEIQLTDAIIELLKQQKVLGLVIDEDSVVFDTGSMEGFALANTYMAVQRLPQI
                     SEKIAPLIQKSDIRPWGFWISLEQGEGYQVKHIYVSPKGKLSMQKHKHRAEIWTVVDG
                     SGFVTVGETVHSAVKGSVFRIEVGEIHRAEGGENGMHIIEVQLGDYLGEDDIIRIEDI
                     YGRN"
     gene            5268..6320
                     /locus_tag="PL056_08870"
     CDS             5268..6320
                     /locus_tag="PL056_08870"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HPM47168.1"
                     /translation="MKLVTVIMAGGSGKRFWPLSRESKAKQSLTLFSEKTLMSETIER
                     ILPLCSDITVVSSIKQKHSIDPDIQKYSFAKVIYEPSGRNTAPCIMLALEFIRSRINE
                     DVAVLVLPADHHIADADEFRKCVEKGVKYLENNRDSIGTIGITPAYPETGFGYIRKGD
                     GIAEGVCSVRSFEEKPDIAKAEMFVNSGEYLWNAGIFLFTLKSMTDQFKELTPDIYES
                     VLNISDFQNIDSGLYGTIRSISFDYAIMEKTVKPVFTVPGDFGWNDVGSWLAYYELMP
                     KDINGNSSRGKAQFIDSKNSLAFNLTDRTIFVFNKTHELVVATDDVILSASLDDHQEL
                     RKVTEFLMNSGAKHFL"
     gene            6337..>7130
                     /locus_tag="PL056_08875"
     CDS             6337..>7130
                     /locus_tag="PL056_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47169.1"
                     /translation="MRKVLLFLVLSVFFQINADNMWINQRQSEFVTASGAFIHVGTKT
                     RGYAMGTAEGSSGTSPYCFVMTDGLTWNDCSPNLVGGMKFVMPARVLPDGKLVGVYME
                     FQGFNSLSSFIVSEDMANFIPVTIFDTKNADDQASSYSEALSVIGNNVWLGLKNGKIK
                     KSTDLGKTFENIVVSSDTEMEISVVKFSDTMNGIAAGGVVKEEMGTEGGTEYDVLAKG
                     AIFRTTDGGSTWTPITQNLEMFPEDVIETSSGRIFLQFYDDDAKNDS"
BASE COUNT         2231 a         1275 c         1635 g         1989 t
ORIGIN      
        1 ctgcagtgat gaatggatgg agtgcaaacc tgaattcgga gcatgcggag caaaatcagg
       61 gagatgtgac acagatgcag attgcagcgg cttggcccct gtctgcaaca caggaactca
      121 tttgtgtgaa tcccccagtt ctcttttgag taacggaagc tttgaaacat ggaccgatcc
      181 tgcatatccc gatttctgga aaggaaccaa gacagatatt gcggcggtaa acatcataca
      241 tgaaacagtt aatgtccatt ccggcagcag tgctttgatg cttgtaaata catcagaaac
      301 gcataaaagg tttacttcac agtcagtggc ccttactgca ggaagctata cctgcaaata
      361 ttttgcaagc ggaagcggtg agataagaaa cgctttttat tctaatggaa ccggtgatta
      421 tatttattca tcatacaccg ttcttgcatc tgtcaactgg acggaaataa catataattt
      481 tactctcgga acagacaggg cggtttttga acttattttc tctgtcagga gtacaactga
      541 caccagcggt catgtaaaag ttgatgatgt cgtctgcaat aagaattgat aatttcccaa
      601 tcctctaaaa ttgactttga tggcttaatt tcatatcatt ttcagtattc aattttcagg
      661 ggtgaaaatg aaatttctct cattcagctt gactgataca ggaaaaaaga gaaggaacaa
      721 cgaagatgcc tttataaata tggctcagat aggacttttt gctgttgcag tcggcatggg
      781 cggagaacaa tgcggagaga tcgcttcgca gacggcagtt gattcgatta cacatttcat
      841 taatgaaaat cacgcaataa tcggtgcttt cgaaatgaat gacagtgctg agaataaaac
      901 gcaggtgctt aacatgcttg cagattctct ccgttttgcg aacatgaagg ttttccggga
      961 agctgaaaag agaaatcttc agggtaaaat gggaacaact ctcactattt ttcttgcgat
     1021 cgataatgcc ggctttatgg tacatgccgg tgattcacgg ctttacatga taagaggtga
     1081 tgaaataaca cagctttcaa cagatcacac tgtttcaaat gattataaaa ggcagtacgg
     1141 tgacgcaggt gaaggttttg atgaaagatt tgcaggaata cttaccaagg cagtgggcat
     1201 acaggaatat gtcgaaccgg agaaactcac ttttacaata gttccgtttg acagatatct
     1261 gctttgttct gacggactct acaatgctta cggcaatgag agggaaaatg ccgaaaaact
     1321 gcttatgtca agagagcacg acattgaaaa agaatcttcg tttctgtcgg atgcggcagg
     1381 tcaactgctt gatatagctt atgaaaatgg tgcttctgac aatgtgtcaa tggttcttgt
     1441 cagtgccttc agcatggatg aagaggaagt gaccggaaca aaggaactta tcaagaagtt
     1501 cgatatagtg agaaatattg accttttcag aaatcttgat tttaaagaac tgctcacagt
     1561 gatggaacgg gttgagataa gaacatataa ccgttatgat gtaatatcaa gaatagaaag
     1621 ccccgacaga gagcttttca ttattcttga aggaaaagtc tcttcactga gaggttcaaa
     1681 acatcttaaa actttttatg aaggcgacca tataggtgaa gtggcatttt taactgaaga
     1741 aaagccgcag tttaatctct ttgtggataa agcgtcatca tttcttgtaa taaaaagatc
     1801 ggtctttata cagatgatga atgataatcc cgctctgggt gtaaagctcc tctggcagct
     1861 tgcaacggtc cttgcaagac agaccaactc atctatcggg ctgataaaag aagaatgatc
     1921 acatctcaga taattgtaac attttttttg actgtatctg ctctgcagtt tgattttgaa
     1981 ggcgggaaca ttgaagatca tatcaataaa aagatcttat atttgcagca gtttacagat
     2041 gaagagagaa tcggtttctg taaggcgggc gatgttttaa gctgcaagat actttttctc
     2101 aaagagataa atgacagaaa cgcttcttct gaaagcatag agggttatct tgaccttttg
     2161 ccggatgagc acagatattc gggaatttat ctttatctta caggaaaatg gaaagatatt
     2221 ccgatagaaa agctcaagat atatatgggt tatcttccgg aagaactttc aaaagggctt
     2281 tataacatct atcttaaaaa gttgtatgca accggaaata ttaaggcctt tatggatgat
     2341 tatattcagg gaaggaatgc tgaacttaca tcatattaca ttaactccat gctcaaaaaa
     2401 gatccgggcg aggcacttga atatttaaga tctctcaagg tcagtttccc tgaaagtttt
     2461 tatgattcga tatccaagga tgtcgaaaaa tatgcgacaa agctcaaagg tgacagctat
     2521 aatgagttca gaatatggaa tctggagttc aattaccgca aagttaggta taatcaggcg
     2581 ataagtcttg catcaaaatg gttcccgtca aaaaaataca gtaacagtta cacatggaga
     2641 gcacatttgt accgcgctat gtcgcataca aaaaaaaggg agcatgacaa agcaagagct
     2701 gtttacggaa aacttgaaga atacttgtca aatgaattac tttcagatgg agatatatat
     2761 aaattttttc atgaatacgg ttattctgaa gcggctctgg gtgataacga caaatcaata
     2821 gagctttatc tcaaaggtta cgaatacttt atctcaaaag atgaggaaag tgccgcaggt
     2881 tttctttata tggcggctga tatgtcacgg cttgcacaat actgggacaa agctgaagaa
     2941 ctttattcgc tctatctcaa aaaccatccc ggatcaggaa gacggaatat ggtggaattt
     3001 ctccttttct ggataaatta caggcagaag gattttatta ctgcaaaagt tcttcttaac
     3061 aatattttag atgggtcgcc tgttatgagt tacgatcacc agagggcttc atactggctc
     3121 gcaagagtta cagcccgcct tggagatacg gtggcatcta tttcacttct atgttcactc
     3181 agtgctaagt tccctgcctc tttttacggt tcaatggccg cttcaaggat aagggaaaac
     3241 gggcttacct gcatggaaaa cgcaaatgaa cagaacagca gttttgaaca gttcaggttc
     3301 agagaggaaa agatgatgcc tgaaaccggt tggcttactg cggcaatggt gacaggagag
     3361 agagctgtca taaaaaaaat gctcaatgca gtttcaaaag ttattaatga aaaaggagat
     3421 gagattgacc ggcttactgc aagttacatt gcaaaaactg tagatgacca cgctctggca
     3481 gctaatctta tcaagtctat cagcaatttt tcaggaacat caaaagaata ttttaaactt
     3541 cagtacacaa tagcatttga agaggagata ttgtcccact gtgattttta cggtgttccc
     3601 ccgatgttcg tgttctctat tgcaaggcag gaaagtcttt tcaatacagg ggctgtgtca
     3661 gcaagttatg caattggact tctccagata cttcctacga ctgctcagct tcttgcaaac
     3721 agagaggatt atggaaagat aaatccggaa gtattgaaaa aaccgcttac caatgtgaga
     3781 ttcggtgtga gattcctttc agatctgctt ataaaattca atggatctat ccctcttgcc
     3841 gccgcatcct acaatgccgg tccaggcagg atcatgaagt ggatggaaaa atcgcctgat
     3901 atggaaattg atgaatttat tgaagatatc cccattttcc agacaaggaa ctatgttaag
     3961 aaagtgatga gcaattatgc ggtatatacc tatatttttg aagacagagt ttatgacgga
     4021 cttgaattca agcttccggt agagtaatat ggcttgtttt tattaaagga gaatgatatg
     4081 aaatattcga taattccggc ggcagggctt gcaacaaggt tccttcctgc gtcaaaggcg
     4141 atacccaagg agctttttcc ggtacttgac aaacctgtca ttcagtacat tgtagaggaa
     4201 gctgtttctg caggcatatc tgcaatagtt tttgtttctg gaagcggcaa agaagcgatt
     4261 ctcgaccatt ttgaccggct taatcctaag ttctctgttt caaagctcaa tgacgaaatc
     4321 aaaagcaagg tggatgagct tgattcgatg atagatgtat tttctgtcag gcagaaagat
     4381 ccgcttggat tgggacatgc cgtacttaaa ggggcatatt cgataaaaga tgagccgttt
     4441 gctgttcttc ttcctgacat gctcattatc cccaggaaag gggtttcttc aatgaaaaag
     4501 atggtggagc tttatgagaa gactggtctt tcagtcattg ccctgatgaa ggttccgcaa
     4561 aaaatgaaag gtctgtacgg tgttgctgaa gtcaagccca cagacgatga gaatgttgtt
     4621 gaaataggca agcttttcga aaaaccgtta caaggtgaaa cggaatctga tcttgctctt
     4681 gtaggaagat atattttcag cccggatatt gtaaacatcc ttaaaaacac aaaaccggga
     4741 aagggtggag agattcagct taccgatgcg atcatcgaac ttctcaaaca gcagaaagtt
     4801 ctcggacttg ttattgatga agacagcgtt gttttcgata caggcagcat ggaaggattt
     4861 gcacttgcaa atacatatat ggctgttcag agactgcctc agatatcaga aaagatagca
     4921 ccgcttattc agaaaagtga catcagacct tggggtttct ggatatcact tgaacagggt
     4981 gaaggttatc aggtgaagca tatttatgtc tctccgaaag ggaagctcag catgcagaag
     5041 cataaacaca gagccgagat ctggacagta gttgacggga gcggttttgt tacagtggga
     5101 gaaacggtac actctgctgt caagggtagt gttttcagga tagaagtcgg tgaaatacac
     5161 agggcggaag gcggtgagaa cggaatgcac atcattgaag tgcagcttgg agattatctt
     5221 ggagaagatg atataatcag aattgaagat atttacggga ggaactgatg aaacttgtaa
     5281 ctgtcatcat ggcgggagga agcggaaaga ggttctggcc attgtcacgg gaatcgaaag
     5341 ccaaacagtc attgacgctg ttttctgaaa aaacacttat gagcgaaaca attgaaagga
     5401 tacttccttt gtgcagtgat attacagttg tttcatcaat aaagcagaaa cactcaatag
     5461 atcctgacat tcaaaaatac agctttgcaa aagttattta tgagccatca ggaagaaata
     5521 cagccccctg cataatgctt gcacttgagt tcataaggtc gcgaatcaat gaagatgttg
     5581 ctgtccttgt tcttccggca gaccaccata ttgccgatgc ggatgagttc agaaaatgtg
     5641 ttgaaaaagg ggtgaaatat cttgaaaata acagagattc aataggaacg atagggataa
     5701 cacctgcata tcctgaaaca ggattcggat acataagaaa aggtgacggg atcgcagagg
     5761 gggtttgttc ggtcagatct tttgaagaga agcctgatat tgcaaaagct gagatgttcg
     5821 ttaattcagg tgaatatctc tggaatgcag gaatattcct tttcactctc aagtcgatga
     5881 cggatcaatt taaagaactc acacccgata tttatgaaag tgttctcaat atcagtgatt
     5941 tccaaaatat cgacagcggg ctgtacggaa ctatccgctc aatttcattt gactatgcaa
     6001 taatggaaaa gaccgtaaaa ccggttttta ctgtgcccgg agatttcgga tggaacgatg
     6061 tcggaagctg gcttgcatat tacgagctta tgcccaaaga tattaacggt aactcctcaa
     6121 gaggcaaagc acagttcatt gattctaaaa attctcttgc ctttaatctc actgacagaa
     6181 cgatatttgt gttcaacaaa acacacgaac ttgttgtcgc aacagatgat gttattcttt
     6241 cggcatcact tgacgaccat caggagctta gaaaagtaac tgaatttctt atgaattccg
     6301 gcgcaaaaca ttttttatga tctttcggga ggcagtatga ggaaggtgct tttatttctt
     6361 gttttgagtg ttttttttca gatcaatgct gataacatgt ggataaatca gcgtcaatct
     6421 gaatttgtaa ctgcaagcgg tgcattcatc catgtcggaa caaaaactag gggatacgca
     6481 atgggtacgg ctgaagggag ctcaggcact tcgccttatt gttttgttat gactgacggt
     6541 ctgacctgga atgactgctc tccgaatctt gtcggcggaa tgaagttcgt aatgccggcg
     6601 agagttcttc cagatggcaa gcttgtcgga gtctatatgg aatttcaggg ttttaattcg
     6661 ctttcttctt ttattgtaag cgaggatatg gcgaacttta ttcctgttac tatttttgac
     6721 acaaagaatg ctgacgatca ggcctcatca tatagcgaag cactttcagt gataggaaac
     6781 aatgtatggc tcggacttaa gaacggaaaa ataaagaaaa gtacagatct tggcaagaca
     6841 tttgagaaca tcgttgtaag tagtgatact gaaatggaaa taagtgtcgt taaattctca
     6901 gacacaatga atggaattgc ggctggcgga gttgtaaaag aggagatggg cacggaaggc
     6961 ggaactgaat acgatgtgct ggcaaaagga gcaatatttc ggacaactga cggaggaagc
     7021 acatggactc cgattacgca gaaccttgaa atgttccctg aagatgtaat agaaacatca
     7081 agcggccgta tttttcttca attttatgat gatgatgcaa aaaatgactc
//
LOCUS       DAOOYB010000143         7003 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_12248_length_7003_cov_9.508060, whole genome shotgun sequence.
ACCESSION   DAOOYB010000143 DAOOYB010000000
VERSION     DAOOYB010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7003
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12248_length_7003_cov_9.508060"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1137
                     /locus_tag="PL056_08880"
     CDS             <1..1137
                     /locus_tag="PL056_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47170.1"
                     /translation="ENMVNITDKTEYFHKDRIGLDKNGHHVWLGYADTNNDYVAPSAP
                     EKTVSSLNCSSTESGLCSINDITLSIKGHSAAYTWQSFSDNTASCSDSKESSGQRYFT
                     ENISLLNNKAGGPQSLLIESGCSYIEYRPAVAYSLTGEIDGYNVFVYPEQMEGTTVYP
                     VKKVKIDKTMLFDKEGPVSGYLNLPPTDTAIHPSGYLVSINRDMGKMEVLDLKNDPGI
                     LADSEGANFVSGKGDREGLLENPVSVAISQSGDIYVLEQGNFRVSAFDINGSVRQIFG
                     DEEELKNYFDISGLDTSAVLLDIDVESTGLIYVLHYVNGGKTKDDYFVQIYSKDGKLI
                     NTNNGIAAAKMAVDHFRNIFTLNYETIVTDITEKPSVSMWIPLP"
     gene            1152..4304
                     /locus_tag="PL056_08885"
     CDS             1152..4304
                     /locus_tag="PL056_08885"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HPM47171.1"
                     /translation="MRNILILMVVFALSGCLEKRSQELIISGSSAKKHKEADLVPESK
                     AVSHNDTVILKLESTDSHEGDTGTTGVDIVTYEFTHSVNQEVCLEKHDDINYHLVIKD
                     SDDKEITKINDGECETVNISEGLYHFHIAHTSLSRSPGETSAVFIRPLAETTHCEDYK
                     SFATQSMPEETVEMKTVSHCTEDPNLICTIPETYKDLPFDCPLKGPWFYSDQRECVTM
                     TRDRVSECYDYLHKNCSSIKSLCINFWQEFSTDNVPYDDLGDSGINVWTKCFGGNANQ
                     YINSKYSNMADPVIKTAFQKLTENFCEEVEADCITGWRIGIGSLVPSECPGFVKYSRY
                     QDLDLKTGEVAILQRTYLHMGRSAILPQNDDIVMVINGQCDNVTHRVGSYIVGPQTQI
                     VLYPFYGFYGKPILFTNDSADHAVYFPVPAISLSERSSLDPTLTGLIYTDTSSISVMS
                     VKGTQEDLCRIKDAGIICDSPTKWDVAPRMTGEVVIVKMADENIKAGKNCSAAFLFDY
                     RCDDINKLGLRSSLSGTLIDVNKAIIFDSNTFLRTFNNTNFEGSAKVSQVSSSVPSTV
                     YLDEEYFQSIHAYKLPDYNHAILISLRKCCGCDLENVSFTGDDLSNTVLVGSNMKNAS
                     FTGVDLTGSDLRETLLTNARFSSVKLGDNYFGCTDFSNVDMSDPSDSSKSTVSVSNSC
                     TWDTSSTYNGITVSCDERTANLTSAKIPVQILPRKSWKTINFKKTTLLDKVDGYDLSG
                     IDLSDSDFTDLKAGGKVMNMQGANLSGSNLTNADFSSIDFSPLIKDKNAQYSNFLNAE
                     IGGTSFANANLEGADFKNVSVDGMTGSVNFSYALMINTVFADADLGRADFSYAYFFSK
                     FRENVATQKKAKAENIVAQDGVFTGSFMSNMKFTNVAFKNGNFNGAQLVGITFSSGDL
                     SNSKFSDAYLQGADLTGALLTDASFSGAYLSLKDGYWHYSSDDAECSDIRINYNKTKL
                     GDTSTVICPNGEKGPCDTDEKLSRKDKKTVEPPCVDGDEDIFGDTDCITHEYLEKNTI
                     PKCDQSNDDVMQCGCLIEGE"
     gene            4397..5977
                     /locus_tag="PL056_08890"
     CDS             4397..5977
                     /locus_tag="PL056_08890"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM47172.1"
                     /translation="MTGGSIIPILIILALILVATVLVFNISLRKLVKKRTAELEREHN
                     FLNYAMEATHTNINITDSDYNLQYVDPNWQKVYGDPTGRKCYEYFMGRDKPCEICGMP
                     QAMEKKETIIYEEVMPNENNRVVEVHTIPFQDSDGKWLFAEFNVDITRRKKAEEVLVT
                     AQRLESLGILAGGIAHDFNNLLSGIFGSIAVASELSENEEAKTYLSQASGSIDRARGL
                     TTQLLTFAKGGVPVKKIGSLSPFIEETVNFALSGSNVVCNFRISEDLSICEFDRNQIG
                     QVIDNLVINAKQAMNDSGTIEVTAENTTISGNEKNELESGRYVRITVKDSGTGIPKEV
                     LAKIFDPFFTTKSKGAGLGLATSYSIVKRHGGFIEVKSEIRKGSTFAVFLPAANGPLP
                     SENENIEKGHKGSGTILVMDDQEIIRITTGKMFETLGYSVVFKEDGASAVEYFEKNRN
                     IKGMIFDLTIPGGMGGLEAVSKIRDIDKEIPVFVSSGYADDPVMADPQKYGFTASISK
                     PFTKAELIRVLNGYMGNK"
     gene            complement(5992..>7003)
                     /locus_tag="PL056_08895"
     CDS             complement(5992..>7003)
                     /locus_tag="PL056_08895"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPM47173.1"
                     /translation="ISGDNINVVSNGIYNIVREHFKYGGKAIIIDEIHKYPNWAQELK
                     NSIDTYKNGKFIISGSSSLDLIKAKYDLSRRVVYYNMKGFSFREYLSFAEGIDLPVLD
                     LNEILKNHSSISTGICSKTKILKHFDEYMTHGYYPFILEGKDEYFQKVLNCIEKVLYE
                     DIAVIFNLTQPKLPILKKMLWLISTSNPFVPNIAGMSRDLGISKEYVYSYLEFLETAG
                     ILNSVNENTEGAKLVRKAGKIYFENPNIPVAINGAIKKETLTGMMRETFFLNQLNLLH
                     KVSLHKQCDFLVDDTFSFEIGGPSKKTAQIREINDGLIAMDGIETGYGRKIPLYLFGF
                     LY"
BASE COUNT         2197 a         1300 c         1475 g         2031 t
ORIGIN      
        1 gaaaacatgg tcaatattac tgataaaacc gaatattttc ataaagacag gataggactt
       61 gacaaaaacg gtcatcatgt gtggctcgga tatgctgata caaacaatga ttatgtcgca
      121 ccttccgcac ctgaaaagac tgtttcatca ttaaactgtt caagtacaga gagtggactc
      181 tgttcgataa atgatataac cctcagcatc aaagggcatt cggcggccta tacatggcag
      241 tcattttcag ataacactgc ttcctgttca gattcaaaag aatcttcagg acagcgttat
      301 tttactgaaa acatttcact tctaaacaat aaagcaggag gacctcagtc ccttttaatc
      361 gagagcggtt gcagttatat tgaatataga cccgccgttg cgtattctct caccggtgaa
      421 atagacgggt ataatgtttt tgtatatcct gagcagatgg agggaactac tgtttacccc
      481 gtgaaaaagg ttaagatcga taaaacaatg ctgtttgata aagaggggcc ggtttcaggg
      541 tatcttaatc ttccgcccac agataccgca attcatccct caggttatct tgtatcaata
      601 aaccgtgata tgggcaaaat ggaagttctc gatcttaaga atgaccccgg gattcttgct
      661 gacagtgagg gtgcaaattt tgtgtccggc aaaggggatc gcgaaggact tcttgaaaat
      721 cctgtttctg tggccatttc tcaaagtggt gacatttatg ttctcgagca gggtaatttc
      781 agggtttcag cttttgatat taacggatct gtcagacaga tattcggaga tgaagaggaa
      841 cttaaaaact attttgacat ttcaggtctt gacacttccg ccgttctcct tgatattgat
      901 gttgaaagca ccgggcttat atatgttcta cactatgtta acggtggaaa aacgaaggat
      961 gattactttg ttcagatcta ttctaaagac ggaaaactta taaacactaa caatggaatt
     1021 gccgcggcaa aaatggcagt tgatcatttt agaaatattt tcaccctgaa ctacgaaaca
     1081 attgtgactg atataacgga aaagccctca gtcagcatgt ggataccatt accttaatac
     1141 ggagaataga tatgcgtaac atattgattt taatggttgt ttttgctttg tcaggttgtc
     1201 ttgaaaaaag atctcaggaa cttatcattt ccggaagcag tgcaaaaaaa cacaaagagg
     1261 cggatcttgt gccggaatca aaggcagtca gtcataatga tacagttatt cttaaacttg
     1321 agagtacgga ctctcatgaa ggggataccg gcacaacagg tgtggatata gtcacttatg
     1381 aatttaccca ttcagtaaat caggaagtgt gtcttgaaaa acatgacgac ataaattatc
     1441 atctcgtaat aaaggattct gatgataaag agatcactaa aataaatgat ggcgaatgcg
     1501 aaactgtcaa catttctgag ggcttatatc atttccacat tgcacacact tcgttgtcaa
     1561 gatcgcctgg agaaacttca gcggtattta taagaccttt agctgaaact acgcactgcg
     1621 aagactacaa atctttcgcc actcaaagca tgccggaaga aacagttgaa atgaaaacgg
     1681 taagtcactg tactgaagac ccgaatctta tatgcacaat acctgaaaca tataaagatc
     1741 ttccttttga ttgtcctctt aaaggtccat ggttttacag cgatcagcgt gaatgcgtca
     1801 caatgactcg tgacagagtt tctgaatgtt acgactatct gcataaaaac tgtagttcca
     1861 taaagtcatt atgcataaat ttctggcagg agttcagtac tgataatgtt ccatatgatg
     1921 atttgggaga ttccggtata aatgtatgga caaaatgttt cggtggaaac gcaaatcaat
     1981 atatcaattc caagtacagc aatatggccg atcctgttat aaaaacagct tttcagaaac
     2041 ttacggaaaa tttttgtgaa gaggttgaag ccgactgtat aaccggctgg aggattggaa
     2101 tcggatcact ggttccctct gagtgtcccg gttttgttaa atattccagg taccaggacc
     2161 ttgatttaaa gacaggggaa gttgcaattt tgcaaagaac ttatctgcat atgggcagaa
     2221 gcgcgattct tcctcaaaac gatgacattg taatggttat caatggacaa tgcgacaatg
     2281 ttacacatcg agtcggttcg tacatcgtcg gacctcagac acagatcgtt ttatatccat
     2341 tttacgggtt ttacggcaaa ccgattctgt ttacaaatga ttctgccgat catgccgtat
     2401 attttccggt tcctgcgatc agcttatctg agaggtcatc tcttgacccg acactgacag
     2461 ggcttattta cacagacact tcatcaatat cagttatgtc agtaaaaggg acgcaggaag
     2521 atttgtgcag aataaaagat gccggaatca tttgtgattc tcctactaaa tgggatgttg
     2581 caccgagaat gacaggtgag gtcgttattg ttaaaatggc ggacgaaaat attaaagcgg
     2641 gcaaaaactg ctcagccgca tttctttttg attacaggtg cgacgatatt aataaactgg
     2701 gacttagaag ttctctttcc ggcactctca ttgatgtaaa caaagcaata atatttgact
     2761 ccaatacatt tttaagaact tttaacaaca caaattttga aggttccgca aaagtctcgc
     2821 aagtaagttc ttccgttcct tcgactgttt atcttgatga agaatatttt caaagtattc
     2881 atgcgtacaa actccctgat tacaaccatg caattcttat aagtcttcgt aaatgctgcg
     2941 gttgtgatct tgaaaatgtc agttttacag gagatgacct ttcaaataca gttcttgtcg
     3001 gatcaaatat gaaaaatgct tcgttcacgg gagtggacct gaccggatca gatttaaggg
     3061 aaactttatt gactaatgcc agattcagct ctgttaaact gggtgataat tatttcggtt
     3121 gtactgattt tagcaatgtt gatatgtctg atccttcaga cagcagtaaa agcacagttt
     3181 cagtttcaaa ttcatgcaca tgggatactt caagtactta taacgggatt accgtttcat
     3241 gtgatgaacg cacagcgaat ttaacatcgg caaaaatacc tgttcagata cttcccagaa
     3301 aaagttggaa aaccataaat tttaagaaga cgacgcttct tgataaagtt gacggttatg
     3361 atctttcggg aattgatctt agcgatagcg actttactga tttgaaagca ggcggaaaag
     3421 ttatgaacat gcagggagcc aacctcagcg gttccaacct tacaaatgca gatttttctt
     3481 caattgattt cagtccgctg ataaaagata agaatgctca atactcaaat ttcttaaatg
     3541 ctgagatcgg cggaacaagt tttgcaaacg ccaaccttga aggggctgat tttaagaatg
     3601 tgagtgttga cggaatgacc ggcagtgtca atttcagtta cgctttgatg ataaatacag
     3661 tttttgccga tgcggatctc ggaagagcag atttttccta cgcctatttt ttctcaaaat
     3721 tcagggaaaa tgttgcgact cagaaaaaag ccaaagcaga aaacattgta gcccaggatg
     3781 gcgtttttac aggaagtttc atgtccaata tgaaattcac aaatgttgca tttaaaaacg
     3841 gtaattttaa cggagctcaa cttgtaggga taactttcag ttcaggcgat ctttccaatt
     3901 caaaattctc tgatgcatac cttcagggag cagatctgac tggcgcattg cttaccgatg
     3961 cttcattttc cggagcatat ctttcactta aagacggata ctggcactat tcaagcgatg
     4021 atgcagaatg ttcagatatc aggataaatt ataataaaac aaagttgggt gacacttcaa
     4081 cagtgatctg tccaaacggt gaaaaaggtc catgcgatac tgatgaaaaa ctcagcagaa
     4141 aagataaaaa gaccgtggaa cctccatgtg ttgacggaga tgaggatatc ttcggcgaca
     4201 cagactgcat aactcatgaa tatctcgaaa agaataccat tcccaaatgt gatcagagca
     4261 acgatgatgt tatgcagtgc ggttgtctta tagaagggga ataagggaaa tttttttctt
     4321 gaaacacatt ctgatatgtg gtataaatct ccctgttttt atttaatatt cagattcgtt
     4381 cccggagaaa aattcaatga cgggtggaag tataataccg attctgataa ttcttgccct
     4441 catacttgtt gcaacagttc tggtttttaa tatttcttta aggaaacttg ttaaaaaaag
     4501 gactgctgag cttgaaagag agcacaattt tcttaattat gcaatggaag ccacccacac
     4561 caacataaat ataacggatt ctgattataa tttgcagtat gtcgatccaa actggcagaa
     4621 agtctacggt gatccgacgg gcagaaaatg ttatgaatac ttcatgggta gagacaaacc
     4681 gtgtgaaatt tgcggaatgc cgcaggcaat ggagaaaaaa gagaccatta tttatgaaga
     4741 ggtcatgccg aatgaaaata accgtgttgt ggaggtccac accatccctt ttcaggattc
     4801 agacggcaag tggctttttg ctgaattcaa tgttgatata acaagacgga aaaaagcgga
     4861 agaagttctt gtaactgctc agcggcttga atcactcgga attcttgccg gcgggatagc
     4921 acatgatttc aataatctgc tcagcggtat tttcgggagt attgccgttg cttctgaact
     4981 gtcagaaaat gaagaagcca aaacctatct ttcacaggca tcaggttcga tagacagagc
     5041 aagagggctt acaacacagc tcctgacttt tgccaaaggg ggtgttccgg taaaaaagat
     5101 aggatctctg tcgccgttta ttgaagaaac agttaatttt gcactcagcg ggtcaaatgt
     5161 tgtatgcaac ttcagaatat ctgaagatct ctcaatatgt gaatttgaca gaaaccagat
     5221 cgggcaggta attgacaatc ttgttatcaa tgccaagcag gcgatgaatg acagcggaac
     5281 cattgaagtg accgctgaaa atacaacaat ttccggcaat gagaagaatg agcttgaatc
     5341 gggaagatat gtcaggataa cagtgaaaga cagcggaaca ggcattccca aagaagttct
     5401 ggctaaaata ttcgatccgt tctttaccac aaaatcaaaa ggtgcaggac ttggacttgc
     5461 aacatcgtat tcgatagtaa agaggcacgg aggtttcatc gaggtgaaat ctgagataag
     5521 aaaggggagc acattcgctg ttttccttcc tgccgcaaac gggccgttac cgtcagagaa
     5581 cgaaaatatt gaaaaagggc ataaagggtc aggcacgatt cttgtaatgg atgatcagga
     5641 aattataagg attacaacag gaaaaatgtt cgaaacgcta ggatattcgg tcgtttttaa
     5701 agaggacggt gcatcggctg tcgaatattt tgagaaaaac cgaaacatta aagggatgat
     5761 ctttgatctc acaattcccg gcggaatggg cggactggaa gcagtttcaa agatcagaga
     5821 tattgacaaa gaaataccag tctttgtttc aagcggctat gctgatgacc ctgtcatggc
     5881 agaccctcag aaatacggtt ttacagcaag catatccaaa cctttcacaa aagcggagct
     5941 gataagagtg ttgaatgggt atatgggaaa taagtagaaa ttgcatcgct gtcaatacag
     6001 aaatccgaaa agatatagcg gaatttttct gccgtaaccg gtttcaattc cgtccattgc
     6061 gatcagtccg tcgttgatct ctcttatctg tgccgttttc ttggacgggc cgccgatctc
     6121 aaatgagaag gtatcatcaa caagaaaatc acattgttta tgaagtgaca ctttgtggag
     6181 taaattgagc tgattaagaa agaatgtctc tctcatcata ccggtcagtg tctctttttt
     6241 tatcgcgccg tttattgcta caggaatgtt cgggttttca aaataaatct ttcctgcttt
     6301 acgaaccagt ttcgcccctt ctgtattttc attaacactg tttaatatcc ctgctgtttc
     6361 aagaaattca agatatgaat agacatattc ttttgaaatt ccaagatcgc ggctcattcc
     6421 ggcaatatta ggaacaaaag ggttgcttgt tgagatcagc cagagcattt ttttcaatat
     6481 cggcagtttg ggttgagtaa ggttgaaaat aacagcaata tcttcataga gaactttctc
     6541 gatgcagtta agaacctttt ggaaatactc atcttttcct tcaagaatga acggatagta
     6601 tccatgagtc atatattcgt caaaatgttt aagtattttt gtttttgagc agataccggt
     6661 tgatattgac gagtgatttt tcaggatctc atttaagtct aataccggca gatcgatacc
     6721 ttcagcaaaa cttaaatatt ccctgaatga aaaacctttc atattgtaat aaaccactct
     6781 tcttgaaaga tcatacttgg ctttaataag atcaagtgaa gatgatcccg atatgataaa
     6841 ttttccgttt ttatatgtgt caatgctgtt cttaagttcc tgtgcccaat tcggatattt
     6901 atgtatctca tcaataatta tggcttttcc gccatattta aaatgttccc tgacaatgtt
     6961 gtaaatcccg tttgaaacaa cattgatatt atcgcccgaa atg
//
LOCUS       DAOOYB010000144         6985 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_12293_length_6985_cov_16.165079, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000144 DAOOYB010000000
VERSION     DAOOYB010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6985
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12293_length_6985_cov_16.165079"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="PL056_08900"
     CDS             complement(<1..611)
                     /locus_tag="PL056_08900"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPM47174.1"
                     /translation="MIKLFRRLFYEIKRLITKKSFIAEIDITDRCNLRCSHCYHFAEK
                     GGDVRKELPLELWKERFGELYSKGIRMVMLMGGEPMLRSDVVMLANQMFPFVEMISNG
                     TIPLPDKLSYRHRIFVSIDGKKETNDLVRGAGVFDKVLENIKEDKRVVLNMTLMESNY
                     QELEYVCELALKLKVSGVVCNIFTAVKGVNESIGTETRKKITD"
     gene            748..2031
                     /locus_tag="PL056_08905"
     CDS             748..2031
                     /locus_tag="PL056_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012609393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HPM47175.1"
                     /translation="MDSLNLRSIKEVDLNGKVVLMRADHNVVKKGKINDPYRIDATIP
                     TIFYITSKGGKIILMTHVGRPKDKKTGEIKTGSDSAIDPVVDYLQQKLGISIKIPDLA
                     PDGTSGITEIGNKMEEMIKDLKSGKTDMLYLPNTRWFAGEEAKGDKSEKFARELAGLA
                     DIYVNDAFGSWQPHASTVHVTKNIPGYAGLLMEKEIENLKKIYEPARPLVSVVAGSKF
                     DTKIEPLSALLEKSDHLVLGGVIYNAYLCAKYGITIKGVGEDDLESAKKFVEFSAKFP
                     GRIVELPFIVESDTMDGKVEGQYRTHDIRTLKPGTALNFVLDADKKSFAESSVTDVFM
                     NAKTIFVNAVMGFTPHFTEGTIAMDKLIDSNKDAMKLFGGGDTLEELKKLLPGVYMKA
                     LDNKKYYFFTGGGAVLTAIEQGTPAGMEPVKVLLK"
     gene            complement(2040..3497)
                     /locus_tag="PL056_08910"
     CDS             complement(2040..3497)
                     /locus_tag="PL056_08910"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPM47176.1"
                     /translation="MKFFSDNISKQYQNLTPLIIYFSALLLKLVLYNFIPLSYDEVAY
                     SVTALNIVQNSSWLNIYNSPDLFFFPPLFNWLSAFFVFIGFEQILAVRTVSMLLSSGI
                     PVIIYLMMKENGFSNLRSIFASFLWTVLPGSIYYSVAGQVETAFLFFILLSAYFIQPH
                     KRNAKNIIISALCFSAAVWIKETALGFSLVFAVIFLLDKEIRKLMIWGFSAFTFCLPL
                     FIQSFIPNEYDLFFELNNDLINWGMISFVKPFENIAVLAGLFSSDKLLMTIFAVLVIA
                     FILISSVIAYKKSSSSFIVRFSIISNLVFIPFFIIFPKKFEYYLLPVLLFSIILFVIS
                     LKEKKEIFILIALVAVFGSYNGLKWRSFIRNYYDETLNFITLVANEKPGATIGTPTPH
                     MAVYIKEKHKLDVKIIPLDFFSGYDPGNCRKKEDRCILQNDYFLSDDEFFAVLFCNKW
                     PVEREKCNIEQMKKVVEKIEKKKKGQIFTLYEVKE"
     gene            complement(3481..>3735)
                     /locus_tag="PL056_08915"
     CDS             complement(3481..>3735)
                     /locus_tag="PL056_08915"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPM47177.1"
                     /translation="AASQMVPEFSEKLVMMKEGEISKPFKTRFGWHIIKLSKVEKKDP
                     PPYNEMRGRLLNVYYQQNMEKAFQNWLGKKKEESRIEILF"
     assembly_gap    3736..3835
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3836..4263)
                     /locus_tag="PL056_08920"
     CDS             complement(<3836..4263)
                     /locus_tag="PL056_08920"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HPM47178.1"
                     /translation="MNFKFFILLFLLIIPFGFSASEQVLNRMVLKVNGKAITLFDLKK
                     AMNPDNPDTVTYQAVVDRKTQLIERVTNDELVRQELELLKMDISNEEIERAAENVATQ
                     NNITIGQLKEEIKSQGLSWDVYKNTILRKQLELLNLKRHIT"
     gene            complement(4270..5352)
                     /locus_tag="PL056_08925"
     CDS             complement(4270..5352)
                     /locus_tag="PL056_08925"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPM47179.1"
                     /translation="MKKMMIVLIAVLVAFVSCQPAGKSSTNLFPQDDGKVIAEFDGIK
                     ITDKYLKTYIDEMNPYLKSRYNSPEKKEELITKIIEGEIIARKAINEGALKDPSLLSK
                     VKSTVARHYSGTKMKEEIEKMLEVSEDDMKKHFEENKASFNQPEKIKASHILIKVDEK
                     KTKEEAKKIADKVLKEAKAGLKDPKSFTELVKKYSDDEGSKRRGGDLGYFERTEEGGK
                     MVKPFSDGAFALKEVGDISDLVESEFGFHIIKLTGKKEKVEKSFEDVKKNIENTLKTE
                     KRKTSFEDMIAKIKTDMGYKFNKEAAAALDLEVPADVQQSSDKFDQRQAPKQQIDPKK
                     IEQLQKMIKKSPEAKNEAAPESVKKQ"
     assembly_gap    5532..5631
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5632..5829
                     /locus_tag="PL056_08930"
     CDS             <5632..5829
                     /locus_tag="PL056_08930"
                     /inference="COORDINATES: protein motif:HMM:NF013804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein"
                     /protein_id="HPM47180.1"
                     /translation="REIRKWDAKVRERGFTVVPVDIYLKDGKAKMTIGLARGKTSFDR
                     KSDLKERDIKRDTARFTEKYK"
     gene            5826..6206
                     /locus_tag="PL056_08935"
     CDS             5826..6206
                     /locus_tag="PL056_08935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HPM47181.1"
                     /translation="MNTRQYGAEAELFTANWLERKGYKILDRNFFMRGGEIDIVAQIN
                     DTIAFVEVRSWDRIFWDGGTPLETINKMKIGHIIKTALYYIQKKRICLQSYNIRFDVA
                     GLIKNDAGAFEIDYIESAFDAEGY"
     gene            6216..>6985
                     /gene="rsmI"
                     /locus_tag="PL056_08940"
     CDS             6216..>6985
                     /gene="rsmI"
                     /locus_tag="PL056_08940"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HPM47182.1"
                     /translation="MSGTLFVIATPIGNMGDITQRAVNILSTCDYILAEDSRVTLGLL
                     QKLGINSKVYSYHKFTEKKFLLKHIDEMKTGKNIGLVSDAGTPAVSDPGKYLVNAALN
                     ENIKVVPVPGPSAAAVAFSCSGSMSDSFVFAGFLPSKGAERIKELEKFLSFGLPVIFY
                     EAPTRIKDLVERLASMKARIVIFRELTKMFEEIFVYDGREIKEKGEFTVVAELMENTN
                     ETKEIDSQLLEILKKSELSNKDAAELVARIYPKLGKNE"
BASE COUNT         2086 a         1340 c         1297 g         2062 t
ORIGIN      
        1 tcatcagtta tcttttttct tgtttcagtt ccgatgcttt cattaacccc tttaactgct
       61 gtaaaaatgt tgcagacaac gccgctcact ttaagtttta aagcaagttc gcagacatat
      121 tcaagttcct gatagtttga ttccatgaga gtcatgttca acacaactct cttatcttct
      181 ttaatgtttt caagcacttt gtcaaaaaca ccggctcctc tgactaagtc atttgtctct
      241 ttttttccat caattgaaac aaaaatccgg tgtctgtaac tcaatttatc aggaagggga
      301 atcgtgccgt tagaaatcat ttccacaaac gggaacattt gattggcaag catcacaaca
      361 tctgatctta acatcggctc tccacccatc agcatcacca tcctgatccc ttttgagtaa
      421 agttcgccga atcgttcttt ccacaattca agaggaagct cttttctaac atctcctcct
      481 ttttcagcaa agtgataaca gtgggaacac cttagattgc atctgtccgt gatgtcaatt
      541 tcagctatga acgatttttt ggtgatcaat ctttttattt cgtaaaaaag ccgtctgaaa
      601 agttttatca tatccattcc tctttaaatc atgctctttt taactgaata aactgcaaag
      661 tgcaagtggt tttttttgac cccgttctaa ttatgatgta ttctctttcc gttcttagcc
      721 gtttaattaa gatatggagg atacacaatg gattctttga atttgcgctc aattaaagaa
      781 gttgatctca atggaaaagt cgttcttatg cgtgcagacc acaatgtcgt caaaaaagga
      841 aaaataaacg atccctacag aattgatgca actattccga caattttcta catcacctca
      901 aaagggggaa agatcatcct gatgacccat gtgggaagac cgaaagataa aaaaacaggc
      961 gaaattaaga ccggcagtga ttcagcgatt gatccggttg ttgactacct tcagcagaaa
     1021 ctcggcattt caataaaaat tcccgatctg gctcctgacg gaacttcagg gatcactgag
     1081 atcggcaaca aaatggaaga aatgataaaa gatcttaaaa gtggaaaaac cgatatgctt
     1141 tatcttccaa atacccgttg gtttgcaggg gaagaggcaa aaggcgataa aagtgaaaag
     1201 tttgcgcgtg aacttgcagg acttgccgat atctatgtca acgatgcttt cggatcgtgg
     1261 cagccacatg cttcaaccgt tcatgtgaca aagaacattc cgggttatgc aggacttctc
     1321 atggaaaaag agatagagaa ccttaaaaag atatatgaac cggcaagacc gcttgtttca
     1381 gttgtcgccg gctcgaagtt tgacacgaaa atagagccgc tttccgctct tcttgaaaaa
     1441 tcagatcatc tggttctcgg cggagttatc tacaacgctt atctctgtgc aaaatacggc
     1501 ataacgataa aaggtgtggg tgaagatgac ctcgaatcag caaagaaatt cgtcgagttt
     1561 tccgcaaaat tccccggcag gatcgtcgaa cttcccttca ttgttgaatc tgacacgatg
     1621 gatggaaaag ttgaaggaca gtacagaact cacgacataa ggacgctcaa accgggaacc
     1681 gcacttaatt tcgttctcga tgccgacaaa aaatcatttg cagaatcaag tgttaccgat
     1741 gttttcatga acgcaaaaac tatttttgta aatgccgtca tgggattcac accgcacttc
     1801 accgaaggga ccatcgcaat ggataagctc attgattcaa ataaagatgc aatgaaactt
     1861 ttcggcggcg gcgacacact tgaagaactc aaaaaactcc ttcccggagt ttacatgaaa
     1921 gcgcttgaca acaaaaaata ctacttcttc acaggcggcg gagcagtcct taccgcaatt
     1981 gaacagggca ctcccgcagg catggaaccg gtaaaagtgc ttttgaagta atctgaattt
     2041 tattctttga cttcgtaaag ggtgaatatc tgtccctttt tctttttctc aatcttttca
     2101 actacttttt tcatctgttc aatattacat ttttctcttt cgacaggcca tttgttgcag
     2161 aaaaggactg caaagaattc gtcatcggat agaaaatagt cgttttgaag gatgcatctg
     2221 tcttcttttt tccggcagtt tccagggtcg tatccgctga aaaaatcaag ggggataatt
     2281 ttgacatcca gtttgtgttt ttcttttata taaactgcca tgtggggagt gggagtgccg
     2341 attgttgctc ccggtttttc atttgcaaca agtgtaataa aattaagagt ttcatcataa
     2401 taatttctga tgaatgatct ccatttcaaa ccgttgtaac ttccaaaaac agcaaccagc
     2461 gctatcagta tgaaaatctc ttttttttcc ttgagcgata ttacaaaaag aattattgag
     2521 aaaagaagca ccggcagaag ataatactcg aatttttttg ggaaaattat aaaaaaagga
     2581 ataaaaacaa ggttggaaat aattgaaaat ctgacaataa aagagctgga acttttcttg
     2641 tatgcaatta ctgaacttat caaaataaaa gcaattacca gaacagcaaa aatcgtcatc
     2701 aacaacttgt ctgaagaaaa aagaccggca agaaccgcaa tgttttcaaa tggtttgaca
     2761 aaagagatca ttccccagtt tatgagatcg ttattcagct caaagaacag atcatattca
     2821 ttcggaatga acgattgaat gaaaagaggg agacagaatg taaaagcgga aaatccccag
     2881 atcattaatt ttcttatttc tttatcaagc agaaaaataa ctgcaaaaac aagtgaaaat
     2941 ccaagagccg tctcttttat ccatactgcg gcagaaaaac aaagtgctga aattattatg
     3001 ttttttgcat ttcttttgtg gggctgaatg aaatatgcac ttaataaaat gaaaaaaaga
     3061 aatgccgttt caacctgccc ggccacactg taataaatcg aaccgggaag aactgtccat
     3121 aaaaatgacg caaaaatgga tctcagattt gaaaaaccgt tctctttcat catcagatag
     3181 ataatgaccg gaatacctga tgacagcagc atggaaacag ttctcacagc cagtatctgt
     3241 tcaaacccga taaaaacaaa aaatgcgctt aaccagttaa aaagaggagg aaaaaagaac
     3301 agatcgggtg agttgtaaat attcagccaa ctgctgtttt gaacaatgtt cagtgcggtg
     3361 acagaataag cgacctcgtc atacgaaaga ggaatgaaat tatatagaac aagcttaaga
     3421 agcagtgctg aaaaataaat aatgagcgga gtaagatttt gatattgctt tgaaatattg
     3481 tcagaaaaga atttcaatcc tgctttcttc cttcttttta ccaagccagt tctggaaagc
     3541 tttttccata ttctgctgat aataaacatt taaaaggcgg cctcgcatct cattataggg
     3601 cggaggatct tttttctcaa cttttgaaag cttgattatg tgccagccga aacgggtttt
     3661 gaaaggtttg ctgatctccc cttctttcat cattacgagt ttttcagaaa attcaggaac
     3721 catctgactt gcggcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngtaat
     3841 atgccgttta agattgagca gttcaagctg ttttcttaaa atggtgtttt tatatacatc
     3901 ccagctaagc ccctgactct ttatctcctc tttaagctga ccaatggtta tgttgttctg
     3961 cgttgcaaca ttttcagcgg ccctttctat ctcttcgtta ctgatgtcca ttttcagcag
     4021 ctcaagttcc tgcctcacaa gctcatcatt ggtaactctt tcaataagct gggtttttct
     4081 gtcaacaacc gcctgatatg tcactgtgtc tgggttatca ggattcatag cttttttaag
     4141 gtcgaacagt gtgatcgcct ttccgttgac cttcagcacc atccggttga gcacctgttc
     4201 agaagccgaa aaaccaaatg gaataattaa aagaaataaa aggatgaaaa atttaaaatt
     4261 catctggaat tactgttttt tgacactttc aggagctgct tcattctttg cttccggaga
     4321 tttttttatc attttctgaa gctgttctat ctttttgggg tcgatctgct gtttgggtgc
     4381 ctgtctctgg tcaaacttat ctgaactctg ctgaacatcc gcaggaactt caagatccag
     4441 agccgcggca gcttccttat tgaatttata gcccatgtct gtcttgatct ttgcgatcat
     4501 atcttcaaaa cttgtttttc tcttttctgt tttaagtgtg ttctcaatgt tctttttgac
     4561 atcttcaaaa ctcttctcga ccttttcctt ctttcctgtg agcttgatta tgtgaaaacc
     4621 gaattcagac tcgacgagat cgcttatatc accaacttct ttaagagcaa aagcaccgtc
     4681 actgaaaggt ttcaccattt ttcccccttc ctcggtcctt tcaaaatatc caaggtctcc
     4741 gcctcttctt tttgaacctt cgtcgtcact gtattttttt acaagttctg taaaactttt
     4801 aggatcttta agtcccgctt tcgcctcttt aagaacttta tcagcaattt tctttgcttc
     4861 ttcttttgtt ttcttttcat caacttttat aagaatatgg cttgctttga tcttttcggg
     4921 ctgattaaag cttgctttgt tctcttcgaa atgttttttc atgtcgtctt cggaaacctc
     4981 aagcattttt tcaatttcct ctttcatttt tgtgccgctg taatgtctgg caacagttga
     5041 cttgactttt gaaagaagtg atggatcttt caaggcgcct tcgttgatcg cttttcttgc
     5101 aatgatctct ccttcgataa tctttgttat gagctcttcc tttttttcgg gtgaattgta
     5161 acggcttttc agataaggat tcatttcgtc aatgtatgtc ttgagatatt tgtctgttat
     5221 ctttataccg tcaaattcag caatgacctt tccgtcatcc tgagggaaaa gatttgtact
     5281 tgattttcct gccggctggc aagatacaaa agcaactaaa acagcaatta aaacgatcat
     5341 catctttttc ataattcaac ctcctgaaat atttagctaa aaaaaacaaa aactgtcaac
     5401 cagtaataaa caatccaagg ggtgtattta atccaattat tttaatatgt caaggatagt
     5461 gggtttttct attcatttca taatttttga atatactttt tttgcgattt tgaggagaaa
     5521 ttgatgggcg annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncgggagata
     5641 agaaaatggg atgcaaaagt gagagaacgg ggttttactg tcgttcctgt tgacatatat
     5701 ctcaaagacg ggaaagcaaa aatgacgatc ggacttgcaa ggggaaaaac atcttttgac
     5761 agaaaaagtg atcttaaaga aagagacata aaaagagata ctgcaagatt tacggaaaaa
     5821 tacaaatgaa tacaaggcag tacggtgctg aagccgaact ttttactgca aactggcttg
     5881 aaagaaaggg ttacaaaatt ctcgaccgca attttttcat gcggggcggc gagatcgata
     5941 ttgtcgcaca gatcaatgac acaatcgcat tcgtcgaagt gagaagctgg gacagaatat
     6001 tctgggacgg cggtacacct cttgaaacca tcaacaaaat gaagatcgga catataataa
     6061 aaacagcact ttactacatt cagaaaaagc ggatctgcct tcagtcatat aacattagat
     6121 ttgatgttgc aggacttata aagaacgatg ccggtgcgtt tgagatcgat tacattgaaa
     6181 gtgcgttcga tgcggaagga tattaaagag gaataatgag cggaactctt tttgtcatag
     6241 caacaccgat cgggaacatg ggtgacatca ctcaaagagc ggtgaatatt ctctcaacct
     6301 gcgattacat acttgccgaa gattctcgtg taactttggg tcttttacag aaacttggaa
     6361 taaattcaaa ggtttattct tatcacaaat tcacagagaa aaaatttctc ctcaaacaca
     6421 ttgatgaaat gaaaacggga aaaaacatcg ggctcgtaag cgatgcggga actccggcag
     6481 taagtgatcc cggcaaatat cttgtaaatg ccgcattgaa tgagaacata aaagttgttc
     6541 cggttccggg tccaagtgcc gccgccgtcg ctttctcatg cagcggatca atgagcgatt
     6601 catttgtttt tgccggcttt cttccgtcaa aaggagcaga aaggataaaa gaacttgaaa
     6661 aatttctctc atttggactt cctgtcattt tttatgaagc tccgacaagg ataaaagacc
     6721 tcgttgaacg gctcgcttca atgaaagcac ggatagtcat tttcagagaa cttactaaaa
     6781 tgtttgagga aatctttgtt tacgacggac gggaaattaa agagaaaggt gaattcaccg
     6841 ttgttgcgga attaatggaa aacactaacg aaactaaaga gatagactct caacttcttg
     6901 aaattcttaa aaaaagcgaa ctttcaaata aagatgccgc tgaacttgtt gcaagaatat
     6961 atcccaaact cggaaaaaat gagat
//
LOCUS       DAOOYB010000145         6916 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_12470_length_6916_cov_9.405626, whole genome shotgun sequence.
ACCESSION   DAOOYB010000145 DAOOYB010000000
VERSION     DAOOYB010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6916
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12470_length_6916_cov_9.405626"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            133..255
                     /locus_tag="PL056_08945"
     CDS             133..255
                     /locus_tag="PL056_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47183.1"
                     /translation="MEKNYLKPEIIEEVSLDRKLVYAGETHDVAGGEPGCQTLS"
     gene            418..798
                     /locus_tag="PL056_08950"
     CDS             418..798
                     /locus_tag="PL056_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47184.1"
                     /translation="MIPINDEKNILEFSEEGILVKSYFPKTDGQPKEYLINHDVTGIL
                     KAVLFPIKEYERDSFIEPLIDKGLIWRKLLTCVAPPITGEDHLFPNYYEMIDRLMDSV
                     PFFNIYHNLKDSPEFIAELLRSIK"
     gene            795..1742
                     /locus_tag="PL056_08955"
     CDS             795..1742
                     /locus_tag="PL056_08955"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPM47185.1"
                     /translation="MKDISLEITDLCNEKCTHCYHPEHKKKGHIKDLDKLDRMLAEFG
                     DLEFMFLMITGGEPLLHPEFKEICKLAQKNRYLISVKTNGTLIDDALVEFFKELKPRT
                     VEVSLYSADPQEHDLITQIPGSFEKSMNGLKKLKKAGVKCSVMTPVLRGIRHWKELLP
                     VMNELQIPWGCGKNIFSSFDDRVEVEQFKGDESHYLDFFEFINTHESQLIENVEDRCF
                     GACGAGKTTVCVAPDYSVRACISYPDSAGIYTGGNAEELLKKARKQLEERFAKLECHD
                     CELVKYCNPCPAHMKIVDGVGICEQTKKEYVKAYLKFFS"
     gene            1821..>3523
                     /locus_tag="PL056_08960"
     CDS             1821..>3523
                     /locus_tag="PL056_08960"
                     /inference="COORDINATES: protein motif:HMM:NF021028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HPM47186.1"
                     /translation="MKKLIFMAMILISFISCSTSVDTGKAPAEEKVIMQTRSVVASDQ
                     GSNYSGGWTDGSNGGSGFGPWIIMINAGTGSAGNFIGDPFAAEITGMSSTSFGLYANP
                     AGSGAVVDASRAFNAPLSTGDTFSFQWGVNWDSNGTGEKGFVMMSGASTLVTIKMAGS
                     GTITINGADMFVNYGVNAMTINCAVLSSTQLRVYATGRDGTETYDNTFTFGETIAPDS
                     IAFYAQFLDTGDQRQPYFDQLKITAIDVSPVPFETKFGVEANWNLISAAESYGEKEYE
                     EENWNFYALNAARATGVETFDGSNYAFRDRSDFLIKNKGSVTALGGFAFQFGDMMDVS
                     EPEIPRNLDVSFNGGANWTRILDMGKSWFEGETGYKELVYIFPTGNDDFSADEFQVII
                     RGNANDTNLIKIGQFKVFSTYTLSFDAQDGFGVFAHKYVANGYVVGELPTPGRTGYKF
                     DGWFTEEDGAGSFVDADYVYALEADSTIYAKWTDVNECDTANGGCAQNCINSIGSHTC
                     SCDAGYSLNIDGFNCDDVNECDTANGGCAQNCINSIGSHTCSCDAGYSLNIDGFNCDD
                     V"
     assembly_gap    3524..3623
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3624..4654
                     /locus_tag="PL056_08965"
     CDS             <3624..4654
                     /locus_tag="PL056_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002634550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="calcium-binding EGF-like domain-containing
                     protein"
                     /protein_id="HPM47187.1"
                     /translation="NCTCDAGYSLNIDGFNCDDNDECTIGTDNCHEFATCGNTIGSFT
                     CTCNEGYYGDGVTCTEYPLGVCQKPEEIVTLPYTHNSTTTGRDSSLTNYGLNCGSVEV
                     SGHPSPDFVYSLELVAGDKVEITLTPVEGFDAVLAVIGTCGEDQDCVMYANENPAAQS
                     EVLYIEAVSDTTYFIVVEGAVESSYGSYTLDVKEWVEETDDDIVPDEDTVDEDVIDED
                     VVDDDTVDEDSDILDDSDMSDDSDQSDESEPSDEDTGDTGDSGDTGDTGDTGDTGNTG
                     DSGDTGDTGDTGNTGDTGDTGDTGNTGDTGDDADEISDEEEDADTETKDEEVYKLGGG
                     GVGCAVVF"
     gene            4737..5276
                     /locus_tag="PL056_08970"
     CDS             4737..5276
                     /locus_tag="PL056_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47188.1"
                     /translation="MRTVKKDLHKDLFAAKSGLIIHSSALCDGSNVYLFGGVSGSGKS
                     SIAKKMNGLMIPVNDEKNVLQFTDRGITVSTYFTVDGSEEKQYLVNENVTGILAKVFF
                     IRKVFNEPSRSEVISDKAQIWKMLLKCVAPPYFGEDHFFPNYLELIEKLIDSVPFFYF
                     YFNISDNPEILADIMRKKC"
     gene            5382..5744
                     /locus_tag="PL056_08975"
     CDS             5382..5744
                     /locus_tag="PL056_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47189.1"
                     /translation="MNRYEDQKREIDLSEIYTGCMAGIKKVAVDQDFFVRPCLDFYER
                     AGKYTGGNAKELFDRSRKMFEEILEKTPCVSCSVAKLCVPCPAHIKIIDGCGSCDESR
                     KQHAKAYCDIFNLTVKGL"
     gene            5793..5927
                     /locus_tag="PL056_08980"
     CDS             5793..5927
                     /locus_tag="PL056_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47190.1"
                     /translation="MKNYEKPAVIEESLIERHLVHATPRTKKDPTCDECPGQAVTSHS
                     "
     gene            5932..6324
                     /locus_tag="PL056_08985"
     CDS             5932..6324
                     /locus_tag="PL056_08985"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HPM47191.1"
                     /translation="MVCSKYSAVINSVLEVHSSGTDELVVASSSMHPTLKTGDKITVE
                     PVEPDKIQVGQVVVFKSESGKLIVHRVVKKEGFVLVTAGDSLRRFDSPVHVYDVIGAV
                     KDLEIARPLSKCFRIIRAIKRRIFKYPF"
     gene            6416..>6916
                     /locus_tag="PL056_08990"
     CDS             6416..>6916
                     /locus_tag="PL056_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47192.1"
                     /translation="MKKFFLMFILLTVVSSLYGEKIYRRDGSFFEVKRNADVFAMKVK
                     PGALKAPENYALNWSFKGGSVVISKDGTGDLPVYLTGAHTVVADSNLFYDGNKDINFI
                     AKKYDLEIVEIWTDYNLVEFAGKGDSIKTAQKIVENGDGFAFANIIRKMDFRANPVKF
                     PVMDKYY"
BASE COUNT         2132 a         1176 c         1543 g         1965 t
ORIGIN      
        1 gtcttattta gattcttata ttaatacaac ttcaataagt ttaaaaatac ttatgtttgg
       61 aaacaacatc atttattgcg acaacacagc caataaagga aattgtaatt tattaaaaat
      121 gggagaatag taatggaaaa aaattattta aaacctgaaa ttatcgaaga agtttcttta
      181 gaccgcaaac tggtttatgc tggagaaacc catgatgtgg ctggtgggga accaggttgt
      241 caaactcttt cataaaatta aaaccagtaa aatattttga cagtcacaat taatgagaat
      301 tgttgcgata tgtttcctgt tcattcttcg tcacttcttt tttttaattt tacgtatctt
      361 ttccctggca tttcagaatc aggaaaatca actttagcaa agaaactgga cggattgatg
      421 attccgataa atgatgagaa aaacatctta gaatttagtg aagaaggaat tttggtcaaa
      481 agctattttc ctaaaactga tggtcaacca aaagaatatt tgatcaacca cgatgtcaca
      541 ggcattctca aagcggttct tttcccgatc aaggaatatg agcgggattc ttttattgaa
      601 ccgttgattg acaagggttt gatctggaga aaacttctga cttgtgttgc tccaccgata
      661 acgggtgagg atcatctttt tccaaattat tatgaaatga tagacaggtt gatggattca
      721 gttccgtttt ttaacatcta tcacaatctc aaagacagcc ctgaattcat cgcagaactt
      781 ttacgatcaa taaaatgaaa gatatctctc ttgaaataac tgatctctgc aacgaaaagt
      841 gtacccactg ttatcacccg gaacataaaa aaaagggaca tataaaagac cttgacaagc
      901 ttgacagaat gcttgcggaa tttggtgatc ttgaattcat gtttttaatg atcaccggtg
      961 gcgaacccct gctccatcct gagtttaaag agatttgcaa gcttgctcag aaaaaccgtt
     1021 atctcatttc agtaaaaact aacggaactc tgattgatga tgcactggtt gaatttttta
     1081 aagaactgaa accccgcact gttgaggtgt cactttattc tgctgaccca caggaacatg
     1141 atttaatcac acaaattccc ggaagttttg aaaaatcgat gaacggattg aaaaaactca
     1201 aaaaagccgg tgtgaaatgt tctgtcatga cacctgtttt aagagggatc aggcactgga
     1261 aagaacttct gcctgtaatg aatgaactgc aaatcccgtg gggttgcgga aagaatattt
     1321 tttcatcatt tgatgatcgt gttgaggttg agcaattcaa aggtgatgag agtcactatc
     1381 ttgatttctt tgagttcatc aacacccacg aaagtcagtt gatcgaaaat gttgaagatc
     1441 gttgtttcgg ggcttgcgga gctggaaaaa caaccgtatg tgtcgctcct gattattctg
     1501 tccgtgcatg catttcatat cccgattctg ccggaattta tacaggcgga aatgctgaag
     1561 aactgctgaa aaaggcaaga aagcagcttg aagagcggtt tgcgaagctt gaatgtcatg
     1621 actgtgaact tgtaaaatac tgcaatccgt gtccggctca catgaaaatc gttgacggtg
     1681 tgggaatatg tgagcagacc aaaaaggaat atgtcaaagc atatctgaag ttcttcagtt
     1741 aaaattgata aaaatcagct cttaaatata atatgtaggc ttaatttgtt tatgtattaa
     1801 ttgtttcctt ggaggatatg atgaaaaagc tgatttttat ggcgatgatc cttatttctt
     1861 ttatttcgtg cagtacttct gttgacacag gaaaagctcc ggcagaagag aaagttataa
     1921 tgcagacaag aagtgtcgtc gcttctgatc agggcagtaa ttacagcgga ggctggacag
     1981 acggctctaa cggaggatca ggtttcggtc cgtggataat aatgataaat gcaggcactg
     2041 gctctgcagg taattttata ggagatcctt tcgcggctga aataaccggt atgtcttcaa
     2101 cctctttcgg actttatgca aatcctgccg gttcaggagc agtggttgat gcttcaaggg
     2161 cattcaatgc accacttagc actggagaca ctttcagttt ccagtggggt gtgaactggg
     2221 attcaaacgg aacgggagaa aagggatttg taatgatgag tggtgcctcc acacttgtta
     2281 caattaagat ggcgggatcc ggcactatca ccatcaatgg tgcagatatg tttgttaatt
     2341 acggtgtaaa cgccatgact attaattgtg cggttttaag ttcaacacag ctcagggttt
     2401 atgcaaccgg gcgagatgga acagaaactt atgacaatac tttcacattc ggtgaaacta
     2461 ttgctcccga cagcatagct ttttatgcac aatttcttga tacaggagat cagagacaac
     2521 catattttga tcagttgaaa ataacagcca ttgatgtaag tcccgttcct tttgaaacaa
     2581 aattcggggt tgaagcaaac tggaacctga tctctgcggc agaaagttac ggggaaaagg
     2641 aatatgaaga agaaaactgg aatttttatg cactgaatgc tgcaagagca accggcgttg
     2701 aaacttttga cggctcaaat tatgcattca gagaccgcag tgattttctc attaaaaaca
     2761 agggatcggt tactgccctt ggcggatttg catttcagtt tggcgacatg atggatgttt
     2821 ctgaacctga aattccccgt aatttagatg tcagctttaa tggaggcgcc aactggacga
     2881 gaattttaga tatggggaaa agctggtttg agggcgaaac gggatacaaa gagctcgttt
     2941 atattttccc gacaggaaac gatgatttca gtgctgatga atttcaagta ataataaggg
     3001 gcaatgcgaa tgatactaac cttataaaga tcgggcagtt taaagttttt tcaacttata
     3061 ctctgagttt tgatgctcag gatggatttg gcgtttttgc acataagtat gttgcaaatg
     3121 gatatgtagt tggtgaactg ccaactccgg gaagaaccgg atataaattt gacggatggt
     3181 tcacagaaga agatggtgcg ggttcttttg ttgatgcaga ttatgtgtat gctctcgaag
     3241 ccgattcaac gatttatgct aagtggacag atgtcaatga atgtgatacc gcaaacggcg
     3301 gatgtgcaca gaactgtata aattctatcg gttcacatac ctgttcatgt gatgcgggtt
     3361 actcacttaa tattgacggt ttcaactgtg atgatgtcaa tgaatgtgat accgcaaacg
     3421 gcggatgtgc acagaactgt ataaattcta tcggttcaca tacctgttca tgtgatgcgg
     3481 gttactcact taatattgac ggcttcaact gtgacgatgt caannnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnntcaactg tacctgtgat gcgggctact cactcaatat
     3661 tgacggcttc aactgtgacg acaacgacga atgcaccatc ggcacagata actgtcatga
     3721 atttgcaaca tgcggaaata cgatcggcag cttcacctgc acatgcaacg aaggatacta
     3781 cggagacgga gtgacatgca cggaatatcc tcttggagtt tgccagaaac ctgaagaaat
     3841 tgttacactt ccatacacac acaactcaac aacaaccggc agggattcat cgcttacaaa
     3901 ttacggtcta aactgtggat ctgtcgaagt ttccgggcat ccatcacctg attttgtata
     3961 ttcgcttgaa cttgttgcgg gagataaagt tgagataaca ctaactcctg ttgaaggatt
     4021 tgatgcagtc cttgcagtca ttggaacatg cggagaagat caggattgcg taatgtatgc
     4081 caatgaaaat ccggcggcac agagcgaagt tctctatatc gaagcagtat cagacacaac
     4141 atatttcatc gttgtagaag gagcagtaga atcatcatac ggaagttaca ctcttgatgt
     4201 taaagaatgg gttgaagaaa ccgatgatga catagttcct gacgaagata ctgtggacga
     4261 agatgttatt gacgaagatg ttgttgatga tgacacagtg gatgaagact ctgatattct
     4321 ggacgattcg gacatgtccg acgactctga ccagtctgac gagtctgagc cgtcagatga
     4381 agacaccggt gacaccggag atagtggtga caccggagat acaggtgaca ccggagatac
     4441 tggtaacacc ggagatagtg gtgacaccgg agatactggc gacactggaa ataccggtga
     4501 cactggagac accggcgata caggcaacac tggcgataca ggtgatgatg ctgatgagat
     4561 atccgatgaa gaggaagatg ccgacacaga aacaaaagat gaagaagtgt ataaactcgg
     4621 cggtggcgga gttggttgcg cagtagtatt ctaaatcatt atatctttct gaaaactctt
     4681 gacagaaaaa caaatctata aataataccg catagtccga tttcggagat ttcgggttga
     4741 gaaccgtaaa aaaggattta cacaaggacc ttttcgctgc aaaaagcggt ttaattatac
     4801 atagcagtgc cctttgtgac ggatcaaatg tttatctttt cggaggtgtt tcgggttcag
     4861 gaaaatcttc aattgcaaaa aagatgaatg gactgatgat ccccgttaac gatgaaaaga
     4921 atgtgctaca gtttactgac agaggaataa ctgtttcaac atattttaca gttgatggca
     4981 gtgaggagaa acaatatctc gttaatgaaa atgtgacagg aattcttgcg aaagtttttt
     5041 ttataagaaa agtatttaat gaaccgtcaa gatctgaagt tatcagcgat aaagcccaaa
     5101 tatggaaaat gcttctgaaa tgcgttgccc ctccttattt cggcgaagat cattttttcc
     5161 ccaattatct tgaattaatt gaaaaactga tagattctgt tccttttttc tacttttatt
     5221 ttaatatcag cgacaatcct gaaatccttg cagatattat gagaaaaaaa tgctgagatc
     5281 tcttgaaatg gaaataacga cattttgcaa tgaaaaatgt attcattgct atcatcccga
     5341 aaatttgtca gcaagaaact tacaaggatt tttttgagtt tgtgaataga tatgaagatc
     5401 agaaaagaga gatcgatctt tctgaaattt atacaggatg catggcagga ataaagaaag
     5461 ttgcagtaga tcaggatttt tttgtaagac cctgtcttga tttttatgaa agggccggaa
     5521 aatacacagg aggaaacgca aaagagctat ttgacagatc aagaaagatg tttgaagaaa
     5581 ttcttgaaaa aaccccttgt gtatcctgca gtgtagcaaa actgtgtgtt ccatgcccag
     5641 ctcacataaa gataattgac ggttgtggat cttgtgatga aagcagaaaa cagcatgcaa
     5701 aagcgtattg tgatattttc aatttgactg ttaaaggatt gtgactaaaa ttgcaatgat
     5761 tctgatgttt aaaattttaa aaaggtgata ttatgaaaaa ttatgaaaaa ccggcagtga
     5821 tcgaagaaag tttgatcgaa agacatctgg tacacgcaac accaagaaca aaaaaagatc
     5881 ccacatgcga tgaatgtccc ggtcaggcag ttacttcaca cagctgatcc agtggtttgt
     5941 tctaaataca gtgcagtcat taactcggtt cttgaagttc attcttccgg cacggatgag
     6001 ttggtcgtcg cttcctccag tatgcacccg actttaaaaa ctggtgataa gataactgtc
     6061 gagccggttg agccggataa aattcaggtc gggcaagttg ttgtttttaa aagtgaatcc
     6121 gggaaactga tcgttcacag ggttgttaaa aaggaagggt ttgtccttgt tacggcgggt
     6181 gacagtttga ggagattcga ttctccggtg catgtttatg atgttatcgg cgcggtaaaa
     6241 gatcttgaaa tagcaagacc gctttcaaag tgttttagaa ttatcagagc aattaaaaga
     6301 aggattttca agtatccgtt ttaattgact aaaaaaataa ggtttgtttt ttgctataat
     6361 taaatgttgg tagtggcatt aagtccgttt acataatttt ttaccggagg gtagaatgaa
     6421 gaagtttttt ttgatgttta ttctgttgac ggttgtttct tcgctgtacg gcgagaagat
     6481 ctacagaaga gacggatcgt tttttgaagt gaagagaaat gcagatgttt ttgcgatgaa
     6541 agttaagccg ggagcactga aagcccccga aaattatgct ctcaactggt ctttcaaggg
     6601 tggttctgtt gttatctcta aagatggaac gggtgacctt ccggtctatc ttaccggagc
     6661 acacacagtt gttgctgaca gcaacctttt ttatgatgga aataaagata ttaactttat
     6721 tgccaaaaag tatgatcttg aaattgttga gatctggact gactataacc ttgtagagtt
     6781 tgctggtaaa ggcgacagca ttaaaaccgc tcagaagatc gttgagaacg gtgacggttt
     6841 tgcttttgca aatatcatca gaaaaatgga tttcagagca aatccggtga aatttcctgt
     6901 aatggataaa tattac
//
LOCUS       DAOOYB010000146         6900 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_12504_length_6900_cov_16.966399, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000146 DAOOYB010000000
VERSION     DAOOYB010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6900
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12504_length_6900_cov_16.966399"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..112)
                     /locus_tag="PL056_08995"
     CDS             complement(<1..112)
                     /locus_tag="PL056_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HPM47193.1"
                     /translation="MRIWSLHPKYLDNIGLVALWRETLLAKHVLSGKTKGY"
     gene            232..1311
                     /locus_tag="PL056_09000"
     CDS             232..1311
                     /locus_tag="PL056_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47194.1"
                     /translation="MKKVMFIIVAALFVLNAGCEKSSTKAENDADLYQPVDKEAVADD
                     EETADNNGVTDNNVTPDENGTTDNNGQPDDPSVDENGVNDENGIPDEDTSAEKEFVAG
                     YEKATEDVFRSIPVAKINAAKEKLHIAYFHSSHGSRVITGMQGLKNYKDGDDTLYDFT
                     TNGTPVAGKLDIYDEYEGGNDLSVKEVVEGNGYSQWYNETVAFLAEAGNSDINVLMWS
                     WCNPDGHNHQKYLDDMEKLIADYPNITFVFMTGHPNGDGESETANSAYQAHKLIRKHC
                     EDNNRFLIDYWDIETHGMEGIYYPNANDNGVSGSTEFYKAWQTSHPGEFFENSCAHAD
                     EDQELTCNRKAYAAWWIWARIAEEK"
     gene            1377..4367
                     /locus_tag="PL056_09005"
     CDS             1377..4367
                     /locus_tag="PL056_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47195.1"
                     /translation="MKKIIVTITSSMLLIIFFSCNSPSHDLNDKDTDLVSDHDNIADA
                     DEISEIEADDSNESDADIDDSYNEFSGLSFKTRPHGITRWDEKFSYFIRCESGKKTET
                     LISVTEDDTCKGTINSDTYVFTPINEKIPSGRCVIAVECSDGENRITQKTHIQIPNPF
                     QFAESFDEPLIFNPIWSKDEYAVFFRDNELFSFNIESDKIELLGSFLGDFSGHFFPVD
                     NFLYYLSQDGMIFKTDGTIQNTTKIEISNESEDNLNFKVIRHVFKNDNILLQTYDEQM
                     QTGFIYYYDSVSKEIIPVELPESWWMGTTCSEKECVAYDFISEKMFGITIPALSASEI
                     CPDEIKNHDGTIVFADNKIFYKYEKTLNSIDIQTCEKTTWSDCENPENVSINRIPGEE
                     VFYTVGNCETSTVLRIFFPDGTDKSTSLNTGVTDIHTNEGVVFFFKYYDENISKPQKC
                     LRVLNTADDSILIPDKICNPDEQIVFSGFFNDSAVFNLFGAELNVSTFLVKSNASIES
                     VAPAFLIKNVQGYFKNAISLNGKDFFTVSTGRYWQMFETDGTKSGTKMRNMITNVWNL
                     KLISASENEVIFDIADDKTHFINSFDITTGKKKTITSKENCKFGNFPFPGTYDYKLRF
                     GNFAFFGVSDTCGVYSLWTTDGTSKGTIDHDIKITHYTQGIGYEIYDPELKAHSDSFG
                     GHYLGKYYFNSLHEKAIDTGVSGYILGFIEDFLIVCSTKSEHVFEITALKKGKIVSSF
                     VYESKGQVSDVSMSGSNIQLLETIGDNETSVANVLFFPDGDLSKKPLFAELFDSGYSS
                     VKKIASNETFTIYSAKIKGASSVALKSVYLAKSGLIIVSEPLYYYNKDSEFNCFSIIG
                     KDLICDYDGVLFSIPVDKKIFAGIRLLEKEGDLRYLGTSFSGEAVIKVSSDSSLVVTG
                     GKKENTKITKIPPYSRISFWNDWILEYEKAFIVEDEKNLLNHVEIKNKELNNQVRYKS
                     ERAIYLFEDPGLYIYNLPKTELFF"
     gene            complement(4356..4940)
                     /locus_tag="PL056_09010"
     CDS             complement(4356..4940)
                     /locus_tag="PL056_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007277761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017128 - phospholipid scramblase activity
                     [Evidence IEA];
                     GO_process: GO:0017121 - plasma membrane phospholipid
                     scrambling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid scramblase-related protein"
                     /protein_id="HPM47196.1"
                     /translation="MSVINRNSFFVKEHVGMFKAANNYDILDPETQEVIMECREETLG
                     MFTKMFRFTDYKRMTPFDITIRTPDGKQVVRVSRGISVFLSDVSVFDENDNKIGGFKQ
                     KLFSIGGKFNVLDNSERQVCQLRGKWTSWDFKFVDGNNEYARVSKEWAGFAKEMFTTA
                     DNYMLTISENVPPDSDIRKLILAAVMCIDMVLKE"
     gene            5092..6822
                     /locus_tag="PL056_09015"
     CDS             5092..6822
                     /locus_tag="PL056_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020885949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HPM47197.1"
                     /translation="MLRIRKIHDPLFPLEKRAVEQVQKLLFERFTDLEKEKIDALPYQ
                     LNNPVKYRFKTILFVADDVSGNVKGFAVLMTDKTLNFCFLDFIATDVTAPSGLGSAIY
                     ERVREEALKLESRGIFFECLPDDPVLCKDCSKLKENASRLRFYERFGAFPIADTKYET
                     PLSEEDDSPPYLMFDGLIKGKTLGKRMAKKIVRAILKRKYGDLCSQEYISLVVNSFKD
                     DPVKLRPPRYIKKPKPAVLIENVSKIALLINDRHDIHHIRERGYVESPVRIRSILSEL
                     EKTTLFEKFQIEEFPEHFIKDVHDSRLINFLKKVCMEIGNEKSVYPYVFPVRNADKPP
                     EELDVSAGYFCIDTFTPLNKNAWLAARNGVNCALTAARIVLNGQKCAYALLRPPGHHA
                     ESKVFGGFCYLNNGAIAANYLSGFGKVAILDIDYHHGNGQQEIFYKRNDVLTVSIHGH
                     PSFSYPYFSGFANEKGEGAGTGFNFNYPLEKNIYGDRYLATLTSALIKIKNFKPSYFV
                     LLLGFDTAKGDPTGSWQLNASDFFNNGKMIGSLKLPTVVIQEGGYKNRSLGINARNFF
                     EGFIKESGDL"
BASE COUNT         2265 a         1233 c         1379 g         2023 t
ORIGIN      
        1 tgtaaccctt tgtttttcct gaaagtacat gctttgctaa aagcgtttcc cgccagagag
       61 caacaagccc gatattatca agatactttg ggtgaagcga ccagatccgc atgaattcct
      121 ctttaaaaaa aaacagataa ttataacaga acctgataaa tattgaataa ttttgagtcg
      181 gggttatcat taagcaggtt ttctttaaaa tttaagaggg gaggtttgca gatgaaaaaa
      241 gtaatgttta taattgttgc ggcacttttt gtgctgaatg ccggttgtga aaaaagttcg
      301 acaaaagctg aaaatgatgc agatctttac cagccggttg ataaagaagc tgttgcagat
      361 gacgaagaaa ccgctgataa caacggagta actgataaca atgtgactcc cgatgaaaac
      421 ggaacgactg acaacaacgg tcagccggat gatccgtcag ttgatgaaaa cggagtgaat
      481 gacgaaaacg gaattcctga tgaagacaca tctgctgaaa aggaattcgt cgccggttat
      541 gaaaaagcga ccgaagatgt tttccgctca ataccggttg caaaaataaa tgccgcaaaa
      601 gagaaactgc acattgcata ttttcactct tctcacggaa gcagagtaat cacgggaatg
      661 cagggtttga aaaattacaa ggatggtgat gacactcttt atgatttcac gacaaatggc
      721 actcctgttg cagggaaact tgatatttat gatgaatatg aagggggaaa tgaccttagc
      781 gtaaaagaag ttgttgaagg taacggttat tcacaatggt ataacgaaac cgttgcattt
      841 cttgctgaag cgggcaacag cgacatcaat gttctgatgt ggtcatggtg taatcctgac
      901 ggacacaacc accagaaata tcttgatgac atggaaaaac ttatcgctga ctatccaaac
      961 atcacattcg tttttatgac aggacacccc aacggtgacg gtgaaagtga gaccgcgaac
     1021 agcgcatatc aggcacataa acttattcgt aaacactgcg aggacaacaa cagattcctc
     1081 attgattact gggacataga aactcacgga atggaaggaa tatattaccc caacgcaaat
     1141 gataacggag tttcaggcag tacagaattc tataaagcgt ggcagacatc acaccccggc
     1201 gaattttttg aaaacagctg tgctcacgcc gatgaagatc aggaactcac ctgcaacaga
     1261 aaagcttatg ccgcatggtg gatctgggca aggatcgccg aagagaaata gagtttttac
     1321 aacttttttc tgataattcc gtttaagaaa aagtggctaa tgatattgag aggaaaatga
     1381 aaaagattat tgttaccata acaagttcaa tgcttttaat aatattcttt tcatgcaaca
     1441 gtccatcgca cgatctgaat gacaaagaca ccgacctggt ttcagatcat gacaacattg
     1501 ccgatgctga tgaaatttct gaaattgaag ctgatgactc caatgaatct gatgcagata
     1561 ttgatgattc ttacaatgaa ttcagtggct tgtcttttaa aacaagacct catggaataa
     1621 caagatggga tgaaaaattc agttatttta tcagatgtga atctgggaag aaaaccgaaa
     1681 ctctcatttc agtcactgaa gatgatacat gtaaaggaac aattaattca gacacttatg
     1741 tttttacacc gattaatgag aaaatccctt caggcagatg cgttatagcg gttgaatgtt
     1801 cagacggtga gaacagaatt acgcagaaaa ctcatattca gatccccaat ccgtttcaat
     1861 ttgctgagtc ttttgatgaa ccattgattt ttaatccgat ctggtcaaaa gatgaatatg
     1921 cagttttttt tcgggacaac gagcttttct cttttaatat tgaatcggac aagattgagt
     1981 tgctgggcag ttttttaggt gacttttcag gtcatttttt tcctgttgat aattttcttt
     2041 attatctttc tcaagatggc atgattttca aaacagatgg tacaattcaa aatactacaa
     2101 aaattgagat ttcaaatgaa tcggaagata acctcaattt taaagtaatt aggcatgtgt
     2161 tcaagaatga taatatttta cttcaaactt atgatgagca aatgcagaca ggtttcattt
     2221 attattatga ttcggtttca aaagaaataa ttccagttga attgcctgaa tcttggtgga
     2281 tggggacaac ttgctcagaa aaagagtgtg tcgcttatga ttttataagt gaaaaaatgt
     2341 ttggaatcac cattccggct ttatcagcaa gtgaaatatg tccagatgaa attaagaacc
     2401 atgacgggac gatcgttttt gccgataata aaatatttta taaatatgaa aaaactttaa
     2461 attcgataga tattcaaacc tgtgaaaaaa caacttggtc agattgtgaa aatccggaaa
     2521 atgtttcaat taatagaatt ccgggagaag aggtttttta tacagtaggc aattgtgaaa
     2581 cttcaacggt tttgcgaata ttctttcctg atggaacgga taaatccaca tctttaaata
     2641 ccggagttac agatattcat acaaacgaag gagtcgtttt tttctttaaa tactatgatg
     2701 aaaatatttc aaaacctcaa aaatgcctga gagtcctcaa tactgcagat gattcaattc
     2761 ttattccgga taagatatgt aatcctgatg aacaaattgt tttcagtgga tttttcaatg
     2821 attctgcggt tttcaattta tttggagcag agttgaatgt cagcactttc ctagttaaaa
     2881 gcaacgcatc aattgaaagt gtcgcccccg cttttttgat aaaaaatgtg cagggctatt
     2941 ttaaaaatgc aatatcctta aatggaaaag attttttcac ggtgtcaacc ggtcgttatt
     3001 ggcaaatgtt tgagactgac ggcactaaat caggaacaaa aatgagaaat atgattacaa
     3061 atgtctggaa tttgaaattg atatcagcat cagaaaacga agttatattt gatatagccg
     3121 atgacaagac ccattttatt aacagctttg atattactac aggaaagaaa aaaacaataa
     3181 catccaaaga aaactgcaag tttggaaatt ttccttttcc cggaacatat gattataaac
     3241 tccgtttcgg aaactttgct tttttcggag tttcagacac atgtggagtg tattcacttt
     3301 ggacaactga cgggacttcg aaaggcacaa tagatcacga tattaaaatc acacattata
     3361 cgcagggtat cggatatgaa atatatgatc cggaactgaa agctcattct gattcttttg
     3421 gcgggcacta tctcgggaaa tattatttta acagcttgca tgaaaaagcg attgatactg
     3481 gtgtgtcagg atacattctt ggctttattg aagacttttt aattgtttgt agcactaaaa
     3541 gtgaacatgt ttttgaaata acagcactca aaaaaggaaa aattgtaagc agtttcgtat
     3601 atgaaagcaa aggacaggtg tctgatgtgt caatgtcagg cagcaacatt caactgcttg
     3661 aaactatagg tgataatgaa acatcagtag ccaatgttct ttttttccca gatggtgatc
     3721 tttcaaaaaa acctctcttt gccgagttat ttgacagcgg ctattcatca gtaaaaaaaa
     3781 ttgcaagtaa tgaaactttt acgatttatt ccgctaaaat aaaaggtgct tcgagtgttg
     3841 cgctgaaaag tgtatatttg gccaagagcg gtctgatcat agtttcagag ccgctatatt
     3901 actacaataa agattcagaa ttcaattgct tttcaataat tggaaaggat cttatatgtg
     3961 attatgacgg agttcttttt tccattcctg ttgataaaaa gattttcgcc ggaataagac
     4021 ttcttgaaaa agaaggggat ttaagatatt taggcacttc tttttcaggt gaagctgtta
     4081 ttaaggtttc atctgacagc tctctggttg ttacgggagg gaaaaaagaa aatacaaaaa
     4141 ttacaaaaat tccaccgtat tcgaggattt ctttttggaa tgactggata ctcgaatatg
     4201 aaaaagcttt tatagttgaa gatgaaaaga acctgctaaa tcatgtcgaa ataaaaaata
     4261 aagaattgaa taatcaagtc cgttacaaat cagaaagggc aatttattta tttgaagacc
     4321 cgggacttta catttataac cttcccaaga ccgaactatt cttttaaaac catatcaatg
     4381 cacatgactg cggcaagaat cagttttctg atatctgaat ccggtggaac attttcgctg
     4441 atggtgagca tatagttatc tgctgttgtg aacatttctt tggcaaagcc ggcccattct
     4501 tttgaaacac gggcatattc gttatttccg tcaacaaatt tgaaatccca gcttgtccat
     4561 tttcctctga gctgacacac ctgcctttcg ctattgtcaa gaacattaaa ttttccgccg
     4621 atgctgaaca gtttctgttt gaacccaccg attttattgt cgttttcatc aaaaaccgaa
     4681 acatctgaaa gaaaaactga aatcccgcgg gatactctca caacctgttt cccgtcagga
     4741 gttctgatcg taatgtcaaa cggagtcatc cgtttataat cggtgaaacg gaacatcttg
     4801 gtgaacatcc cgagtgtttc ctcgcggcat tccatgatca cttcctgagt ttcagggtcc
     4861 agaatgtcat agttgtttgc cgccttgaac atacccacat gctctttcac gaaaaaactg
     4921 tttctgttga taacgctcat tgacacctct ttaaaaaaat taagcacttg cctcaacaag
     4981 aatagataaa aatagcagtt ttacaagaaa acgaactttg tttgtccaaa aaatactctt
     5041 tttctacagc tgcaatataa tcagtatgta catttcatct gaggtgaaac aatgctccgg
     5101 atcagaaaaa ttcatgaccc ccttttcccg cttgagaaaa gagctgtcga gcaggttcag
     5161 aaacttcttt ttgaaagatt cactgatctt gaaaaagaaa agatcgatgc actaccgtat
     5221 cagctcaaca accctgtgaa atacaggttt aaaaccattc tttttgttgc tgatgatgtt
     5281 tccggaaatg ttaagggttt tgcggtgttg atgaccgata aaacgctgaa tttctgtttc
     5341 cttgatttca ttgcgacaga tgtaaccgct ccaagcggac ttggaagtgc aatttatgaa
     5401 agggtcagag aagaggcact caaacttgaa agccgtggaa tattttttga atgtcttccc
     5461 gatgatccgg tactttgcaa agattgttcg aaattgaagg aaaatgcgtc ccggcttaga
     5521 ttttatgaga gattcggagc gtttcccata gcagacacta aatatgaaac acctttgagc
     5581 gaagaagatg attccccccc ttatctgatg tttgacggac ttataaaagg aaaaactctc
     5641 ggaaaaagga tggcaaaaaa aatagtcaga gcgatactta aaagaaaata cggggatctc
     5701 tgttcacaag agtatatttc acttgttgta aattccttta aagatgatcc ggtaaagctt
     5761 cgccccccca gatacattaa aaagccgaaa cctgctgttt tgattgagaa tgtttccaaa
     5821 atagctcttt taataaatga cagacacgac attcatcaca taagggaaag aggttatgtt
     5881 gaaagccctg tgaggataag atcgatcctg tcagagttag aaaaaactac tctttttgag
     5941 aaattccaga tcgaagaatt tccggaacat ttcataaaag atgtccatga cagccggctg
     6001 ataaattttc tgaaaaaagt gtgcatggaa attggaaatg agaagtcagt ctatccttat
     6061 gtttttccag taagaaacgc tgataaacca cctgaggagc tcgatgtaag tgccggttat
     6121 ttctgcattg acacatttac tccgctgaat aaaaatgcat ggcttgcagc aaggaacggt
     6181 gtcaactgtg ctcttactgc ggcaagaatc gtgctgaacg gtcaaaaatg tgcatatgca
     6241 cttcttcggc ctccgggaca ccatgctgaa tcaaaagtgt tcggcggatt ctgttatctc
     6301 aacaacggtg caattgcggc aaactatctg agcggatttg gaaaagttgc aattcttgac
     6361 attgactatc atcacgggaa cggacagcag gagatatttt acaaaagaaa tgatgtgctg
     6421 actgtttcta tccatggaca cccgtcattt tcatatccct acttttcggg atttgccaat
     6481 gaaaaagggg agggggccgg aacaggattc aattttaatt accctctgga aaagaatatt
     6541 tacggagaca gatatctggc aacacttacc agcgcattga taaagatcaa aaattttaaa
     6601 ccctcatatt ttgttctact tcttggtttt gacacagcaa aaggagatcc caccggttca
     6661 tggcagctta atgcgtcaga tttctttaat aacggaaaaa tgatcgggtc tcttaaactt
     6721 ccgaccgtag ttattcagga aggtggttac aaaaatagaa gtctcggcat aaacgcaaga
     6781 aatttttttg aaggttttat taaggaatca ggggatttat aatttctggt actcataagc
     6841 tccgacatcg gcaatgccgt ttgagatcct ttcgtttccg tcaagatcct cagaaaaact
//
LOCUS       DAOOYB010000147         6878 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_12554_length_6878_cov_8.833797, whole genome shotgun sequence.
ACCESSION   DAOOYB010000147 DAOOYB010000000
VERSION     DAOOYB010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6878
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12554_length_6878_cov_8.833797"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1062)
                     /locus_tag="PL056_09020"
     CDS             complement(<1..1062)
                     /locus_tag="PL056_09020"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPM47198.1"
                     /translation="MRKGLVLLIFITASFFIYPFLVFGEFDDELFPEDQPEAGGGDNA
                     VVEKSLSDLDLKSLVDGCSSSNDESFKEIDKRCGLGQIDSCRAAASLFERFCNEGIVS
                     SCEKLVDVFERGLLVDGKIVFKKDPRKAVMVAKKACDLDVGLCLTFGSRYLFGFASGY
                     RTDPFAGQFLLDKACSSGSPEACNFLGRAYSSGDVLKKDPVLAFKYYFAACKISPEST
                     LWCNMGFSYEDGDGVPRNYGYAFKFYDFLCSSGNSMYCNSCGLFYEKGTGVKKSLEMA
                     RILFDKGCLLGGADSCTNLARFYYLGIGVKKDLYMSVLFRKKGCDFGSPDSCLVMAVF
                     YEKGEGVPKSIEIRDSLLFK"
     gene            complement(1180..2010)
                     /locus_tag="PL056_09025"
     CDS             complement(1180..2010)
                     /locus_tag="PL056_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47199.1"
                     /translation="MKNAGLFFCIVVFSFGLNAKSCFKASVVKPSPFMGNNGEIFELS
                     DGSLWEVENEYEYLYEYYPSVVVCPSKGKLIIKKKSLDIKLVSAARISKQSDSKSPIS
                     KQTDFIKTNDWELYEETSLQGSVSGTVKKGSIFKTTSGNIYEVTGFTLQLVMELQPEV
                     VILRKGNIYKLVVEGFDEPLVCKCLSCSSERSETLPKNEPTTEVSSVIESIIISDFKG
                     LKHGNIYKLANGQIWEQTEAWIWIWIWVNPSVIIWKEGGGFRMKVENIEHAVSVQRVK
                     "
     gene            complement(2339..>3017)
                     /locus_tag="PL056_09030"
     CDS             complement(2339..>3017)
                     /locus_tag="PL056_09030"
                     /inference="COORDINATES: protein motif:HMM:NF025085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HPM47200.1"
                     /translation="QIDFQCKMDFSRENIRQFIINLIGDYKNMLTDSVLEVFNKMSSK
                     YNWLDESSNNIYLYNGWKTNKSWFVNKKVVLPVYGYSHPFLDWNGKWRLNRSDVQEVV
                     GDIDKVMNYFDGKRNYLSICDALERALDEGQTRNIESTYFIISVFKKKTIHLTFKDEN
                     IRRRFNVMACKGKNWLPPSYGVKRYSDMTTEEKEVVDSFEGELSYNLNINQIGFENSE
                     LLQIEAG"
     assembly_gap    3018..3117
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3118..3773)
                     /locus_tag="PL056_09035"
     CDS             complement(<3118..3773)
                     /locus_tag="PL056_09035"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HPM47201.1"
                     /translation="MFESNQFYPTPENLIRKMWYKLDENRINFILEPSAGKGHILKYL
                     SEKRKRFEVSCIEINPEFQAILKSKGHNLIDSDFLSYSGLDRFDCILMNPPFANGEAH
                     LLKAINLMFNGQIVCLLNAETIKNPFTNTRRELVKMLEELGADIEFIQNGFLEAERKT
                     AVEVALIYIDIQRDIKTVLFDNLKEAQNPETVEIEENSDIEKRDRIDIEVGNYEQDIE
                     A"
     gene            complement(3789..4013)
                     /locus_tag="PL056_09040"
     CDS             complement(3789..4013)
                     /locus_tag="PL056_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47202.1"
                     /translation="MMMIDLVRLAGPYTQKTKDGKPYQVWKVILLNRETGINFKVYNC
                     LTYERASELTEDISYDRDIQSDIELNKLTA"
     gene            complement(4025..4204)
                     /locus_tag="PL056_09045"
     CDS             complement(4025..4204)
                     /locus_tag="PL056_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47203.1"
                     /translation="MSREDFVCFCCGMKGSDEAKEYEHEEYEALICINCGSFWDYSGF
                     QEATDWSKEFVNLEN"
     gene            complement(4201..4386)
                     /locus_tag="PL056_09050"
     CDS             complement(4201..4386)
                     /locus_tag="PL056_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47204.1"
                     /translation="MKDLKTVRKIIKKLDEVETLFQALSQKTKDELEREFEGGTKMYL
                     INMQRNFNDLTKERGGK"
     gene            complement(4415..4732)
                     /locus_tag="PL056_09055"
     CDS             complement(4415..4732)
                     /locus_tag="PL056_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47205.1"
                     /translation="MRDNEKESVLDLIPDYGFIIEYYRKNRPDQKDGSNIEAVTELIT
                     SWIHYEKPYKKENIENTFKDIETNALSWAYANKRRFERFERSLKKVCEIADIKDVPKI
                     LVN"
     gene            complement(4755..5216)
                     /gene="radC"
                     /locus_tag="PL056_09060"
     CDS             complement(4755..5216)
                     /gene="radC"
                     /locus_tag="PL056_09060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HPM47206.1"
                     /translation="MTSSVNFKGMSKKELVAFLEELHCCEDRETVLVSKDVASIFHGK
                     IGFKCKQEHFLAMYLDGANKVIETRIIFIGTLNKSMVHPREVFAPAFELRAASIIIGH
                     NHPSGNPEPSIEDTLMTRKLKEAGEILGIEILDHVIFTDTDHYSFQGNGNF"
     gene            complement(6289..>6878)
                     /locus_tag="PL056_09065"
     CDS             complement(6289..>6878)
                     /locus_tag="PL056_09065"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HPM47207.1"
                     /translation="VNGRNKRSFRMKNILISLVFVLLFVMVSCDSGTKIDTGNTGDSG
                     NTGDTGNSGDTGDICPENDKFCHSQDGLDWSDVSSSYIDWNKAIKYCEDLGGRLPTIS
                     ELRTLIQNCSGTETGGECGVTDSCLSSEDCWNDPCYGCEYDESGKYSVFGDTYWFWSS
                     SEWSVHTNYAWYVDFHYGPVLSYYKDYYHSVRCLR"
BASE COUNT         2081 a         1520 c         1098 g         2079 t
ORIGIN      
        1 tttaaaaagc agactgtcac gaatttctat gctcttgggt acaccctcac ctttttcata
       61 aaaaacagcc attaccaaac aagaatccgg agaaccgaaa tcacatcctt tttttctaaa
      121 aagaactgac atatacaaat ccttcttaac gccaatgcca aggtaataaa aacgagccag
      181 atttgtacat gaatctgcac cacccaaaag acagcccttg tcaaaaagaa tacgagccat
      241 ctctaaactt ttcttaacac cggtaccttt ttcataaaaa agaccacaag aattacaata
      301 catcgaatta cccgaactgc acagaaaatc ataaaactta aaagcatagc catagttacg
      361 aggaacgcca tcaccatcct catagctgaa acccatatta caccacagcg tagattcagg
      421 cgatatttta caagcagcaa aataatattt aaacgctaac acagggtctt tcttcaaaac
      481 atcaccagaa ctataagctc ttcccaaaaa attgcatgcc tcaggagagc cagaagaaca
      541 cgccttatcc aaaaggaact gcccggcgaa aggatcagtc ctataaccag atgcaaaacc
      601 aaacaaatac cgagaaccga atgttaaaca caatccaaca tccaaatcac acgccttttt
      661 tgcaaccata acagccttgc gaggatcctt tttaaaaaca atcttgccat caactaaaag
      721 accgcgttca aaaacatcaa ccaacttttc acaggaagaa acaatgccct cattacaaaa
      781 acgctcaaat aatgaagccg ccgccctaca ggaatcaatt tgtcctaaac cgcatctttt
      841 atcaatctcc ttgaaagatt catcattact ggaagaacaa ccatcaacta aagactttaa
      901 atccaagtca gataaagact tttcgacgac ggcattatca ccgccgccag cttccggctg
      961 atcttcagga aaaagctcgt catcaaactc gccaaaaacc agaaaaggat aaataaaaaa
     1021 tgatgccgta ataaatatta ataaaactaa accctttctc atatctctcc ccaaaaaaat
     1081 taaacacaac ggaagcattt taacagattc aaatcagaat taaagtttta aaatggaggt
     1141 ggagcggtaa gaaggggcgg gggcaccttg tttttgggtt tatttgactc tctgaactga
     1201 aactgcgtgc tctatgtttt caactttcat tctgaatcca ccgccctcct tccaaattat
     1261 aacagaagga ttgacccaaa tccaaatcca aatccaagcc tcagtctgct cccaaatttg
     1321 tccgttggcg agtttgtata tatttccatg tttaagccct ttaaaatcgc tgataattat
     1381 gctttctata accgaactga cctcagttgt gggctcattt ttgggtaaag tttcactcct
     1441 ttctgatgaa caactaaggc atttgcaaac cagcggttca tcaaatcctt caacaaccaa
     1501 tttatatata ttaccttttc tcaggatcac tacttcgggt tgaagttcca tcacaagctg
     1561 taaagtaaat ccagtaacct cataaatatt cccagatgtt gttttaaaga tacttccttt
     1621 ttttactgta ccacttactg aaccttggag actagtctct tcatataact cccaatcgtt
     1681 agttttgata aaatctgtct gtttggaaat tggtgacttc gaatccgact gtttagaaat
     1741 tcgagcagct gaaaccagct ttatatccag agattttttc ttgataatta atttcccttt
     1801 actcggacaa acaacaacac taggataata ttcatataaa tattcatact cattttcaac
     1861 ttcccataaa gatccgtccg aaagttcaaa tatttctcca ttattgccca taaatggcga
     1921 tggcttaaca acagatgctt taaaacagct tttagcattc aaaccaaagg aaaaaacaac
     1981 tatacaaaaa aacaggcctg catttttcat aaaacactcc ttaacacttc aaaacattat
     2041 attttattat aaataaaact caaaaaaaac ggctgaaagc caaatggtac aggtgcgatc
     2101 acctgtcgcc ggagagtcca gaggggtgac ggaaacgccc tctggtatct ataaactcac
     2161 agttcactct taacccgtga ctgttcacgg tcacgcagtg acacagtgcc ttcctgccga
     2221 tcaaaaaagc agcccttcga gcacctcatg gcaagccggg agtccagagg gtggagcgcc
     2281 tctggtcaaa aaaagagcgg gtttccccgc tcctgactta aaaaagggtg gtgattattt
     2341 atcctgcttc aatttgcagg agttcgctgt tttcaaagcc gatctgatta atattgagat
     2401 tataggataa ttcaccttca aagctgtcaa caacttcttt ttcttcggtt gtcatgtcgc
     2461 tgtatcgttt aactccgtaa cttggcggaa gccagttttt ccccttgcat gccattacat
     2521 tgaaacgcct tctgatgttt tcatctttaa atgtcaggtg aatagttttt ttcttgaata
     2581 cggaaataat aaaataggtg ctttcaatgt ttcgggtctg tccttcgtca agtgctcttt
     2641 caagtgcatc gcagatactt aaataatttc tttttccgtc aaaataattc atcactttgt
     2701 caatgtctcc cacaacttcc tgaacatctg acctgttcaa tctccatttt ccgttccagt
     2761 caagaaacgg gtgactgtat ccgtaaactg gaagaacgac ttttttgttt acaaaccatg
     2821 atttattggt tttccagccg ttataaagat aaatgttgtt gctcgattca tcaagccagt
     2881 tatattttga gctcatttta ttgaaaacct caagaacgga gtccgtcagc atgtttttat
     2941 aatctccgat cagatttatt ataaactgtc ggatgttctc cctgctgaaa tccattttac
     3001 actggaaatc tatctgannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngct
     3121 tcaatgtcct gttcatagtt tccgacctca atatcaatcc tgtctctttt ttcaatatcg
     3181 ctgttttcct caatctcgac cgtttcgggg ttctgtgctt ctttaagatt atcaaaaaga
     3241 acggttttaa tatctctctg tatatcaata taaatcaatg caacctcaac agcggtcttt
     3301 ctttcggctt ctaaaaagcc gttttgaatg aattcaatat ccgctccaag ttcttcaagc
     3361 atttttacaa gttcacgcct tgtatttgta aaggggtttt taatggtttc ggcatttaaa
     3421 agacagacaa tctgaccgtt aaacatcaga tttatcgctt tcaataaatg ggcttccccg
     3481 ttggcaaaag ggggattcat taagatgcag tcgaatctgt caagtcctga ataactcaga
     3541 aaatcactgt caatcagatt atgaccttta cttttaagaa tcgcctgaaa ttcagggttt
     3601 atttcaatac aggaaacttc aaaccttttc cttttttcag aaagatattt taaaatgtgt
     3661 cctttccctg ctgatggttc aagaataaaa tttattctgt tttcatcgag tttataccac
     3721 atttttctaa ttaaattttc tggtgtcgga taaaattgat tactttcaaa cattgttttt
     3781 ttctcctttt aagctgttaa tttatttaat tcgatatcgc tctgaatatc acggtcataa
     3841 gatatgtctt ccgtgagttc agaagctctt tcataagtca ggcagttata aaccttaaaa
     3901 ttgatgcccg tttccctgtt taataaaata actttccaga cctgatacgg cttgccgtcc
     3961 tttgttttct gtgtgtaagg tcccgccaac cttacaagat caatcatcat catttttaac
     4021 ctctctaatt ttccagattc acaaattctt tagaccagtc ggtcgcttcc tgaaatcccg
     4081 aataatccca aaaacttccg caattaatac agattaaggc ttcatactct tcgtgttcgt
     4141 actctttcgc ctcgtcgctt cctttcattc cgcaacagaa acagacaaaa tcctctctac
     4201 tcattttcca cctcgttctt ttgttaagtc attaaaattc ctttgcatat ttataagata
     4261 catttttgtt ccgccttcaa actctctttc aagttcgtcc ttagttttct gagaaagtgc
     4321 ttgaaaaagt gtctcaactt catcaagctt tttaataatt tttctgactg tctttaaatc
     4381 cttcattttt aaacctcttt ttttaccaat aaaatcaatt gactaatatt ttcggaacat
     4441 ctttaatgtc cgcaatttca caaacctttt tcagggatct ttcaaaccgt tcaaaccgtc
     4501 ttttatttgc ataagcccag cttaaagcat ttgtttcaat gtctttaaat gtattctcaa
     4561 tattttcctt tttatatggc ttctcgtaat gaatccagct tgttatcagt tctgttactg
     4621 cttcaatatt tgaaccgtct ttctggtctg gtctgttctt cctgtaatat tcaatgatga
     4681 atccataatc aggaataagg tctaaaacac tttctttctc gttatctctc attttcttcc
     4741 tctctccttc tattttaaaa attaccgttc ccctgaaaac tgtaatgatc tgtgtccgta
     4801 aaaattacat ggtcgagaat ttcaattcct aaaatctcgc cagcttcttt aagttttctt
     4861 gtcattaaag tatcttcgat tgaaggctca gggtttccgc tcgggtgatt gtgtccgatt
     4921 atgattgaag cggctctcag ttcaaaagca ggggcgaaaa cctctctcgg atgcaccatt
     4981 gatttattca atgttccgat aaaaatgatt cttgtttcaa ttaccttgtt ggctccgtca
     5041 aggtacattg caagaaaatg ctcctgttta catttaaaac cgatttttcc atgaaagatt
     5101 gaagcaacat cttttgaaac cagaacagtt tccctatctt cgcaacagtg aagctcttct
     5161 aaaaatgcca ctaattcttt tttactcata cctttgaaat ttactgaact tgtcataatt
     5221 gctaacctcc aaaccgttaa aaaagaccct attacccctg aaaaaaattt tttcagacgg
     5281 aagagatgcc taacggcgag tgttcccctc ccaaaaagcg tatgaaacgg aacggctgtg
     5341 agaggatcgg aaagcggaca agtacagcat gcacaggaaa ttttaccgga gtgtaacaaa
     5401 cacggaggct tgattatttt gggggaaacc ttcaggggga accgaaataa ttcaaatttt
     5461 cgagaactga cttgcgtgaa gtggagtagc aaacttggta ataatcttcc gtctgaaaat
     5521 ttgagctata cgcagtatca ttattttctc tttaaatgtc ttaaattctg gaaggtgcaa
     5581 aatccggttt attgagctaa atcataacct tacatatatt cccgtgggga attctcccta
     5641 aatgtttaag aaacccgcat atgaagaata tatttaataa aaacaatcat ttaaaagttt
     5701 atgtggcggc tggtgacagc ttcgcttcca ctgccctatt ttcgctgtta aaatgctccc
     5761 tgtaatttat ctgaaacact gtatcatcaa tacagaccgc cgcccgaagg gcgggcgggg
     5821 gacaatagaa accggagcgg gacggacttt actgtttaaa ggttagcaac gataaacttg
     5881 actaacgcaa gcagaatctc tataattata actaagtatg gggtgctctt tcgagcactc
     5941 tttttttttg gtacacttct ggcggaaaag agaaggctcc ttgacggagc gttgtcccga
     6001 ctgaagcaca gaggctgtgc ccgtcgtgag accttaaaga aatatttccg tcagctgtct
     6061 ggagggtgcg aagcagttcc tttcagaagg aaagaaccgc aacgatagtg gagtccgtaa
     6121 gggagcgccc ggacggcctg aaacaattac aaaaaacata tttcataaat aattcacata
     6181 tatcatttaa atatcagata tttacggaat aaatacagaa tatatatcaa acccaaaatg
     6241 ttccgctcca agagcgaaac aaagaaataa ccaaataaaa atcatggtct agcgcaaaca
     6301 ccggacactg tggtagtagt ccttgtagta gctgagcaca ggtccatagt ggaaatccac
     6361 ataccatgcg tagtttgtat gcacagacca ctccgaagac gaccagaacc aataagtatc
     6421 tccaaatacc gaatatttgc cggattcgtc atattcacat ccgtaacatg gatcattcca
     6481 gcaatcctca gaagataaac aactatccgt cactccgcat tcaccacctg tttcagttcc
     6541 agaacaattt tgaatgagtg ttcgaagttc cgagattgtc gggagccgtc cgccgaggtc
     6601 ttcacaatac ttaatcgctt tattccaatc tatataacta cttgaaacat ctgaccaatc
     6661 cagtccgtcc tgggaatgac agaacttatc attctcagga caaatgtctc cagtgtcgcc
     6721 ggagtttcct gtgtcaccag tattgccact gtcgccagtg tttcctgtgt caatctttgt
     6781 tcctgaatcg cacgaaacca tcacaaacaa cagaacaaaa accaatgaaa ttaaaatgtt
     6841 cttcatcctg aaactccttt tattccgtcc gttcacag
//
LOCUS       DAOOYB010000148         6872 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_12566_length_6872_cov_8.632536, whole genome shotgun sequence.
ACCESSION   DAOOYB010000148 DAOOYB010000000
VERSION     DAOOYB010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6872
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12566_length_6872_cov_8.632536"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            332..1447
                     /locus_tag="PL056_09070"
     CDS             332..1447
                     /locus_tag="PL056_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013944591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM47208.1"
                     /translation="MKREQKEQILKDLEKKIVFIVGPRQVGKTWLAKEIAKDFKKPVY
                     LNYDRAEDRSIIKNESWLTSTDLLIFDELHKMKGWKNYLKGVFDTKPEQMKILVTGSA
                     RLSTFKKSGDSLAGRFFSHTLLPFSPSELKDTEFSKNIERFMERGGFPEPFLAENIND
                     SNRWRLQYIDGLIRTDILDFEKVHDLRALETVLELLRGRVGSTVSYSSIANDVGIAPN
                     TVKKYIQIFEALYIVFRLTPFSKNIARSLMKEPKIYFYDTGLVNGDNGAKFENLVAVS
                     LLKHVLAKRELEGIAWDLNYIRTKDKKEIDFCIVKNGDAKHFIEAKFSDPSVTPALAA
                     FCEKFKVPATQVVFDLKRERKESNFEIVTASSFLNEI"
     gene            complement(1485..1868)
                     /locus_tag="PL056_09075"
     CDS             complement(1485..1868)
                     /locus_tag="PL056_09075"
                     /inference="COORDINATES: protein motif:HMM:NF016834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lecithin retinol acyltransferase family protein"
                     /protein_id="HPM47209.1"
                     /translation="MGDHIRVKRPFYYHHGIYVSDSEVIHFNTHGQGDGLLGDAVIIS
                     STLKEFLDLGKCEVKSYGLSLLEKPYPPEIVVERARSRLGESGYNVAINNCEQFAEWC
                     KTGMHRSRQIEHIFIKILGKVFKRK"
     gene            complement(1934..2356)
                     /locus_tag="PL056_09080"
     CDS             complement(1934..2356)
                     /locus_tag="PL056_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HPM47210.1"
                     /translation="MNDKKKNAVNEVFENSDKYSKHYDEESFFKKILNFGKKAGAKVV
                     FAALLLFYAVKSDKMPMKDKLIVFGALGYFIIPIDLIPDLIPILGYSDDFAVLFATVR
                     MIAMYIDPQVINDAKFRLSEWFGEIDDKDLEQVLKILS"
     gene            complement(2433..4205)
                     /locus_tag="PL056_09085"
     CDS             complement(2433..4205)
                     /locus_tag="PL056_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47211.1"
                     /translation="MLINMSSNIIGFGGNTIVESGCKDIFDYNNKEAANYCNENRLRH
                     DALRYSMVSLKSYEDFFNKGQGPFYKKLQKLEESIKKEDLDCNINRSWPNPALSESRG
                     TCYVLGDENFWNNHLNGISTLIEKVKTYDITSICNNMMVLYSKRMGNDQISGSIPDFC
                     KNESKQKNPEIKNDVSGLWDLIQINTTTIFNFLLGFLSAFLLLLVERLMNIFALNKFY
                     TRKKEYDKLEKQLRNMYSIGSLTNDDVRRYHEFFNKTEIFLSDLEKEKDNIYDILTLF
                     RGSGNTIKTNSIVPRRILKMFNMSKSYIWIDADKKSCLLETLSDLTKVAENKISKKEN
                     SDRNIKLNSIKKVFVITDGSTHDFVAAKGNCLLESREIKITPLSDVYKEGRKTLPDAY
                     HCKKQISEISKVLEKEAGDLQIMIGGMTGKLGFSWYLAQQEFSNRINVLRYTIRNTQK
                     QVEILLNRRRSFSGNDLTEYKEVFLPKKERNGVVIGIYLHNHHSKKEFKNNLQTYAKK
                     NNLKFEYFCKDLKEDELPELLSFISDRTINTINSICDSEGGQKVYIAILSYWPVTFVV
                     GSALKNKKINLLQWDGKSHKLMSP"
     assembly_gap    4402..4501
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4502..4756)
                     /locus_tag="PL056_09090"
     CDS             complement(<4502..4756)
                     /locus_tag="PL056_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47212.1"
                     /translation="MEFYQIIIYASIVIGSIFLILLLADNILKLLNTLVENLRKTINN
                     ICSQLFADILKFFLILGFAGFFMALIFIDGYFTFKSVGYLT"
     gene            5014..5667
                     /locus_tag="PL056_09095"
     CDS             5014..5667
                     /locus_tag="PL056_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47213.1"
                     /translation="MSDLNKALTTKDIDKIIQNKPSISDSTSEKDIINNFFTNYIRRS
                     KESGAIYFVEWKNLVAQDKEYGEFHRKSIEEAKKFSKKVCSAHPAIKYVGFFKSTETF
                     WFSGTGNKYTAEKHMIKASGGKGRIDPLILIKGSCGCYICKYLEFRRKCVDIKNHNVF
                     RTLRKSAKRSFPDKDAYYVVRFKARFKVRIIPQIESYVGSVPGDVLFEIRERCQENG"
     gene            5633..6349
                     /locus_tag="PL056_09100"
     CDS             5633..6349
                     /locus_tag="PL056_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47214.1"
                     /translation="MKFEKGVRKMDKFTEERYQGLFIYFKYIYYKNGWDKTLGKDRDL
                     KELFQSALIMDYKLNRENITLEDLISPYIFDSRFANEGQYKGWLAKIFINFLKDYNRN
                     INKETYQTISITNEDYSEMEFESFVSTPEETIHLDNLKNMAINTATSKFVRKECEVLS
                     LYLNEEKTYEEISRVLDRSVSGVWRDVERILKELKKTIENDLPEGKFSDDDRTNYLRY
                     FLEYIEKVDWDKILRSKKKL"
     gene            6346..>6872
                     /locus_tag="PL056_09105"
     CDS             6346..>6872
                     /locus_tag="PL056_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47215.1"
                     /translation="MTEKNNYTSEPSFKKFFKRPEKPSTIKSGQIWTVFPDNKSIFSD
                     NPENTGTVVLIYEASDESAFIVPIHPYKYLRPKTDIIIKKDVHFSGLSSNELVASIHL
                     ALSISITAFEYGVYLGEFTKKGMIETKDALKNFENILFHLALAQRKDSRNEMMTDEES
                     RNTVLAGIYDLVPVDG"
BASE COUNT         2315 a         1160 c         1111 g         2186 t
ORIGIN      
        1 ctgaaaagtt gaaaaacttt gcacaagtcc tgtataatca ggcacttctc atggaaggaa
       61 ttgcaccaaa agacccagtc aaatttgcct caatgatctc tgaattcatg tcggaagtac
      121 cggtttagaa agcgacttaa taatttccca gtccgatgaa catgggctaa gataacacga
      181 gccttcagcc cgctttttct gctaacgcct tgcttcccgt cacgaaagaa tctttaaagc
      241 tttaaaattt gtgtgactaa actgtctgac tattttgcag cgtattgcaa aaaagtcttg
      301 actattttgc attttgttgc agaatagtcg tatgaaaagg gagcagaaag agcagatcct
      361 taaagatctt gaaaagaaaa tagtttttat tgtcggtcca agacaggtgg gtaaaacctg
      421 gcttgcaaaa gagatcgcaa aagattttaa aaaaccggtt tatcttaatt acgatagagc
      481 ggaagacagg tccattataa agaatgaaag ctggctcact tcaactgatc ttcttatttt
      541 tgatgaactt cacaaaatga aagggtggaa aaattatctt aaaggtgttt ttgatactaa
      601 gccggaacag atgaaaattc ttgttacggg aagtgcacga ctttcaactt tcaaaaaaag
      661 cggtgattcg cttgccggaa gattcttttc acacacgctt cttccctttt ccccatctga
      721 gctgaaagat actgagtttt ccaaaaatat tgaaagattc atggagcggg gcggttttcc
      781 tgagccgttt cttgctgaaa acattaatga ttccaatagg tggcgacttc agtatattga
      841 cggattaatc cgaacagaca tccttgactt tgagaaagtt catgatctga gagctcttga
      901 aacagttctt gaacttttaa ggggcagagt cggttcaaca gtttcatatt cctcaattgc
      961 aaacgatgtg ggaattgctc caaacactgt taaaaaatat attcagatat ttgaagcact
     1021 ttatattgtt ttccgactga cccctttttc aaaaaatatt gcaagatctc ttatgaaaga
     1081 gccgaaaata tatttttatg atacaggtct ggttaatgga gacaacggtg cgaaattcga
     1141 aaatcttgtt gcagtttctc ttttaaagca tgttcttgca aaaagagagc tggaaggaat
     1201 tgcatgggat cttaattata taaggaccaa agataaaaaa gagatagatt tctgtattgt
     1261 aaaaaacggt gatgcaaaac attttatcga agcaaaattt tctgatccat ctgtaactcc
     1321 tgctttggca gctttttgcg aaaaatttaa agtccctgcc acacaagttg tctttgatct
     1381 taaaagagaa agaaaagaat caaattttga gattgttact gcttcttcat ttcttaatga
     1441 gatttagatt gtactgactc tttttcaaaa aacatcttta caaactattt tcttttaaac
     1501 actttcccaa gtatttttat gaagatatgt tcgatctgac ggcttctatg cattcctgtt
     1561 ttgcaccatt cagcaaactg ttcacagttg ttaatggcga cattataacc gctttctcca
     1621 agccggcttc ttgctctttc aacaacaatt tcaggcggat acggcttttc aagcaaactc
     1681 agcccataag atttgacctc acatttcccc agatcaagaa actctttcag cgttgaagaa
     1741 attataaccg catcaccaag caacccatct ccttgaccat gagtattgaa atggataact
     1801 tccgaatctg acacataaat cccgtgatga taataaaaag gtcttttcac cctgatatga
     1861 tctcccatca ccggggattt gaaaatccaa tcattttgat ttgttaagtt tttactcatt
     1921 cacggtttac acgctaagac aaaatcttta agacctgttc aaggtctttg tcatcaatct
     1981 caccaaacca ttcactcagc cggaattttg catcatttat cacctgcgga tcaatataca
     2041 tcgcaatcat tctcacagtc gcaaaaagaa cagcaaaatc atcactgtag ccaagaatag
     2101 gaattaaatc aggaatgaga tcgatcggta taataaaata accaagagcc ccgaaaacta
     2161 ttaatttgtc tttcatcggc attttgtcgc tttttactgc atagaaaagg agcaaagccg
     2221 caaaaacaac ctttgctcca gccttctttc caaaattgag aatcttctta aaaaaagatt
     2281 cctcatcata atgctttgaa tacttatcac tgttctcaaa aacttcgtta actgcgttct
     2341 ttttcttgtc gttcatgggc acctcctttc aaaaaaacat tcagaacttt gatttctcca
     2401 attttatctc agagaaaaaa tatgccaaat agttagggcg acattaactt atgagatttt
     2461 ccatcccatt gaagaaggtt tatcttttta ttttttaaag cactgcctac cacaaaagtt
     2521 accggccagt aactcaatat tgctatatat actttttgtc caccttctga atcacatatt
     2581 gaatttattg tatttatggt tctgtctgaa ataaaactca gaagttcagg aagctcatct
     2641 tctttaagat ctttacaaaa atattcaaac ttcaagttgt tcttttttgc ataggtctgc
     2701 aaattgttct tgaactcttt ttttgaatgg tggttgtgaa ggtaaatgcc tataacaacg
     2761 ccatttctct cttttttagg aagaaaaact tctttatatt cagttagatc gttaccggaa
     2821 aatgatcttc tcctgttcaa tagaatttca acctgctttt gagtgtttct tatggtatat
     2881 cttaatacat ttattctgtt tgaaaattcc tgttgcgcaa gataccatga gaatccgagc
     2941 tttcctgtca ttcctccaat catgatctgt aaatcccccg cttctttttc taaaacttta
     3001 gaaatctcgc taatttgttt tttacagtga tatgcatcgg gcagtgtctt tcttccttcc
     3061 ttatacacat ctgaaagggg agttatctta atttctctgc tttccaataa acaattccct
     3121 tttgcagcaa caaaatcatg agtggatcca tctgtgatta caaaaacctt tttaatactg
     3181 ttcaatttga tgtttctatc tgaattttct tttttgctga ttttattttc ggccactttt
     3241 gtcaaatcag aaagtgtttc cagtaaacaa ctttttttat ccgcatcgat ccaaatataa
     3301 gacttggaca tattaaacat ttttagaatt cttctcggaa caatactatt tgttttaatt
     3361 gtatttccgc ttcctctgaa aagagtaagg atgtcataaa tgttgtcttt ttctttttcc
     3421 agatcactca aaaagatttc tgttttatta aagaattcat ggtatcttct gacatcatcg
     3481 ttcgttagag atccaattga atacatattg cgtagctgct tctcaagttt atcatactcc
     3541 ttttttcttg tataaaattt attcaaagca aaaatattca ttaacctttc caccaaaaga
     3601 agaagaaaag ctgaaagaaa tcccagcaag aaattaaata ttgttgttgt gtttatttga
     3661 atcaaatccc aaagacctga cacatcgttt tttatttcag gatttttttg tttagattcg
     3721 tttttacaaa aatcggggat actccctgaa atttgatcgt ttcccattct tttgctataa
     3781 agcaccatca tgttattgca aatacttgtt atgtcataag tttttacttt ttcaattaat
     3841 gtgctgattc cgttgagatg attgttccag aaattctcat cacccagaac ataacaggtt
     3901 cctctagatt cagaaagagc aggattgggc caagatctgt taatattgca atctaaatcc
     3961 tcctttttta tagactcttc caatttctga agctttttat aaaaaggccc ctgtccttta
     4021 ttgaagaaat cttcgtaact tttcaaagaa accattgaat acctgagggc gtcatgtctt
     4081 aatctgtttt cgttacagta gtttgcggct tctttgttgt tgtaatcaaa aatatctttg
     4141 cacccactct caacaatggt attgcctcca aaacctataa tattgctcga catatttatt
     4201 aacattatta tgagatacac agtctggcac atatacagaa aaataatcat ttgctgtttt
     4261 ctaagaatta aaattcccaa aggaagtatg ttgaaaagac caactactgt caatgccgca
     4321 aaaactctgt aggaaaaatc ccagctagta agataaccta cagatttaaa agtaaaataa
     4381 ccgtctataa aaataagagc cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nagtaagata acctacagat ttaaaagtaa aataaccgtc tataaaaata agagccataa
     4561 aaaagccggc aaaccctaga attaaaaaaa actttaatat gtcggcaaaa agttgggagc
     4621 atatattgtt tatggttttt cttaaatttt caaccaaagt gttaagtaat ttaagaatat
     4681 tgtcagccag aagtagaatt aaaaatatag accctatcac tattgaggca tatataataa
     4741 tctgataaaa ctccaagaaa tcctccctaa aaaccgattg ttttataaat ttttaaaatg
     4801 acatacatcg cgtcctccag tcaaaaaact cactctcttg tgaatagaat ggattagcat
     4861 catgtttttt tcgggaaaga aaacataaat gagttaagtg cttaaaatat ttaaagtaaa
     4921 ttcccgcagc caaattaagc aaaaagattt ttctttgaca aatatttggt tttatctaat
     4981 atgcaaaagt gattatattc ttaggtttaa agaatgagtg acttaaataa ggcattaacc
     5041 acaaaagaca ttgataaaat tattcaaaat aagccgtcca taagtgattc gacaagcgag
     5101 aaagacataa taaacaattt tttcacaaat tacatccgaa gatcaaaaga atccggagcc
     5161 atttattttg ttgaatggaa aaatcttgtt gcacaggata aggaatacgg agagtttcat
     5221 agaaaaagca ttgaagaagc aaaaaaattc agcaaaaaag tatgcagtgc tcatccagct
     5281 ataaaatatg ttggattttt taaatcaact gaaacttttt ggttttcagg gactggaaat
     5341 aaatatacag cagaaaaaca catgattaaa gcatcaggcg gaaaaggaag aattgatcca
     5401 ttgatattaa ttaaaggttc ttgcgggtgt tacatctgca aatatttaga atttagaaga
     5461 aaatgcgttg atattaaaaa ccacaatgtt ttcagaacat taagaaaatc tgcaaaacga
     5521 agttttcccg acaaagatgc ttactatgta gtaaggttta aagccaggtt taaagtcaga
     5581 ataataccac aaattgaatc ttatgtagga agtgttcctg gagatgtttt gtttgaaatt
     5641 cgagaaaggt gtcaggaaaa tggataagtt tacagaagaa agatatcagg gattattcat
     5701 ctattttaaa tatatatatt ataagaacgg gtgggataaa actctgggga aagacagaga
     5761 tttaaaagaa ctttttcagt cagcgttgat aatggactat aaattgaatc gagaaaatat
     5821 tactttagaa gacttaattt cgccttatat ttttgacagc cggtttgcaa atgaaggtca
     5881 atataagggc tggcttgcga aaatattcat aaacttttta aaggattaca atagaaacat
     5941 aaataaagaa acttatcaaa caatttctat tacaaacgaa gattatagtg aaatggaatt
     6001 tgaaagtttt gtttcaacac cggaagaaac tattcatctg gacaatttaa aaaatatggc
     6061 aataaataca gcaacttcaa aatttgtgcg aaaagaatgt gaagtgcttt cgttatattt
     6121 aaatgaggaa aaaacttatg aagaaatttc ccgtgttttg gacagatcgg tatcaggagt
     6181 ttggagggat gtggaaagaa ttttgaaaga gctaaagaaa acaattgaaa atgatcttcc
     6241 tgaaggtaaa ttttcggatg atgacagaac aaattatttg agatattttc ttgaatatat
     6301 tgagaaggtt gattgggata agattttgag gtcaaaaaag aaattatgac agaaaagaat
     6361 aattacacat cagaaccaag ttttaaaaaa ttctttaaaa gaccggaaaa accttcaaca
     6421 ataaaatctg gacaaatatg gacagttttt cctgacaata aaagcatttt ttcagataat
     6481 cccgaaaaca caggaacagt tgttcttata tatgaagcga gcgatgaatc tgcatttata
     6541 gttccgattc atccttataa atacctcaga ccgaaaacag acattattat aaaaaaggat
     6601 gttcatttct caggactttc ctcaaatgaa ctggttgctt ccatacattt ggctctttca
     6661 atttcaataa cagcttttga atatggagtt tatcttggtg aattcacaaa aaaaggaatg
     6721 attgaaacaa aagatgcttt gaaaaatttt gaaaacattc tttttcacct tgctcttgct
     6781 caaagaaaag attcaagaaa tgaaatgatg acagacgaag aatcaaggaa tactgttttg
     6841 gctggaattt acgaccttgt tccagttgat gg
//
LOCUS       DAOOYB010000149         6851 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_12616_length_6851_cov_8.381989, whole genome shotgun sequence.
ACCESSION   DAOOYB010000149 DAOOYB010000000
VERSION     DAOOYB010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6851
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12616_length_6851_cov_8.381989"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="PL056_09110"
     CDS             complement(<1..280)
                     /locus_tag="PL056_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47216.1"
                     /translation="MKKLLFMALILLTFISCNSDKPKNDTDINDKDSGITDVDIPVPD
                     PDTVVIDVEQPDIDADADTNVPCLDLRYNENTIKTNFPFKDKNGKPTFC"
     gene            complement(277..777)
                     /locus_tag="PL056_09115"
     CDS             complement(277..777)
                     /locus_tag="PL056_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47217.1"
                     /translation="MKKSLVILTVFFIAVISVWFLSAENENKENVWRIKIHNNESAKM
                     SIYSTVSLTLEDLKKSKFCPDGKCPEEFNYCMTSYVSVVEMGRLGSEGFCDLGSDYSM
                     ESEKKWKRKISFKQLEKLLENYPEKGTRPFLMKFIILSDSPEPKKENLIGPFLFKTDY
                     NWLGDI"
     gene            1022..1372
                     /locus_tag="PL056_09120"
     CDS             1022..1372
                     /locus_tag="PL056_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47218.1"
                     /translation="MADQNIKIDYKKLNSFLKDYIRMMKKGWLFLIMNDDYNPGDSIL
                     FNIKVAGLEKELTASGNVVYKGPNEKGSDGIGLKFSFDERSSELLSVNLKKLILEKYG
                     ETWGGRICSLMGTD"
     gene            1373..2572
                     /locus_tag="PL056_09125"
     CDS             1373..2572
                     /locus_tag="PL056_09125"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HPM47219.1"
                     /translation="MTSNFKTSSRFCVLDDEKNFLSVAKGQLSKYLPEIEGFYSTDPE
                     EVYKFAAQDRLTLYIIDMNLGESNGMEIYRKIASISYRARVIFITGDIHFLEDEDIRR
                     QTLSEGGMDFIEKPIKWHEMAIKMKNHLNLLEYQFQLEEKVEERTQMLIHADRLATVG
                     TMVSSIVHEISSPLTFIKANQETFLFAYNKVKDSLTDPEAKKVFENFILPGVKDSLSG
                     IQRIEELLKSFRRFYKKEHKISVNDIASIINEVKNLTYYGIKRGGITFSYQIKDDSSN
                     SIKCNRQELIQVLTNIVNNAIDALDEVRQENKELKISVKPEGQFINITVSNNGPAIPE
                     AVFDKIFNPFFTTKKEDKGTGLGLSIVHQIIKIMGGSVYAVNRSEDHDTVDFVLKLPV
                     NQDPKDN"
     gene            2588..3328
                     /locus_tag="PL056_09130"
     CDS             2588..3328
                     /locus_tag="PL056_09130"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HPM47220.1"
                     /translation="MKTEAIVLAAGKGTRMKSSMPKVMHNVLSKPMIGWIMESLDPVA
                     DYINVVTGHGRDQVEKYLSDDYKSIKFSFQQKQNGTGGAVKSAVPNIAGDASHIIICT
                     GDTPLIKTDTFRNAIEQFTKSGSDLTVVSTILEDAGHYGRIVRNSRNEVESIVEYLDA
                     DESQMKIREINSGIYIVEKKFLVEAVFKIKNNNSKYEYYLTDIVKIANRMNKKVTAYV
                     ENDSFSLSGINDKEQLAQVEAEMLKRMQ"
     gene            complement(3400..3678)
                     /locus_tag="PL056_09135"
     CDS             complement(3400..3678)
                     /locus_tag="PL056_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009147409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HPM47221.1"
                     /translation="MNIYVGNLAYKMSEEDLRAAFEAYGTVDSARFVTDRETGRSKGF
                     GFVEMPNQDEALAAINALNGTELFGRTVTVNEARPREERKPGGYGKKY"
     gene            3868..4650
                     /locus_tag="PL056_09140"
     CDS             3868..4650
                     /locus_tag="PL056_09140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPM47222.1"
                     /translation="MIFFEKIGNFLIRAFNDVSKYIAFTYQCLFYMFAPPYRFGQVAD
                     HTERIGIQSIVIVALSTFTTGMILSLQLINIMRLFGAESFAGAGVAISMSRELAPVIT
                     AIVLIAKNGSSMTAEIGSMKVTEQIDAMETMAVSPVRYLVIPRIFASLMVFPALTALA
                     NVVGIYGGYFVANTSAMINSTSYMHTMYFWVDPEDIWTGLVKAFFFGFIVTTICCYHG
                     LKTKGGAKGVGEATTTAVVISTVAILIADYIMTYIFINFFGL"
     gene            4654..5412
                     /locus_tag="PL056_09145"
     CDS             4654..5412
                     /locus_tag="PL056_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013682559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPM47223.1"
                     /translation="MENIIVKVRDLHKSFRDIKVLEGVNIDIEEGKVTTIIGKSGTGK
                     SVLLKNIIGIMKPEKGEIFFKDIDITKLSDHKLLEIRKNIGYLFQDAALFDFMNVEEN
                     ITFPLVEQLGIKDGKLLKERVGKLLELVELPGIEKKFPSELSGGMRKRVGLARAIAVE
                     PKIVLFDEPTTGLDPILAESINTLIMKVNKELGMTCVVISHDIASTFRRADKVALLYD
                     GTIQFFGEPAKAVISDHPVLKQFISNSFRPLGKE"
     gene            5417..>6851
                     /locus_tag="PL056_09150"
     CDS             5417..>6851
                     /locus_tag="PL056_09150"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HPM47224.1"
                     /translation="MTRAAKIGVFFTVCIVMVSIYIVKSADNFTGGSTRVLYALSDDA
                     MGLLNDSDIRIAGVVVGKMEKIELEEGKAKMTLNIWDEVDVYENARIEKVMESMLGTY
                     VLVLDPGTKDARKLEEFEYIKNVISSSGINGAMDKASAMMEKATEAISILTNDGNQEK
                     LNKIIDALSRTAENTSQTFEGSMKLLLETLKNMSEITHKINVRSENEMDKLSKILENT
                     VALTDKMNSMMKDKDEEISETLTALRDSLKLISEELKASRGAMQNLRDISEDVNKITG
                     KVANGEGNIGKIVNDEKLYNDLTKISDKLTDYIDTTLGMQIHVDAHSDYMVFDRAFQT
                     YFDITLQPRADRFYMFGVVDDSQGRVTETSTTYELGVDDGSSVQNYVITENKTKTDSE
                     LKFNLQIGRNFGPMTFRGGIFQNKAGLALDYNPWKFLSISAEIFDSGGDVPELRVKGL
                     ARPLIWTIEPLSWIYLTVGGENLISGER"
BASE COUNT         2281 a         1284 c         1367 g         1919 t
ORIGIN      
        1 ggcagaatgt tggtttgcca tttttatctt tgaaagggaa atttgtcttg atcgtgtttt
       61 cgttgtatct gagatcaaga catggaacat tggtgtcagc gtcagcgtca atatccggct
      121 gttcgacatc aattacaaca gtatctggat ctgggactgg aatatcaaca tctgtgatcc
      181 ctgaatcctt atcgttaatg tcagtgtcat tcttcggctt atctgaattg caggatataa
      241 atgtcaacaa aatcaatgcc ataaataaaa gttttttcat atgtcaccaa gccagttata
      301 atcagtttta aaaagaaacg gacctatcag gttctctttt tttggttccg gactgtctga
      361 aagaataata aacttcatta aaaacggtct tgttcccttt tcggggtagt tttcaagaag
      421 tttttccaat tgtttaaaac ttatttttct tttccatttc ttttctgatt ccatgctgta
      481 atcacttcca aggtcacaaa acccttcact tccgagacga cccatttcaa caacacttac
      541 ataacttgtc atacagtaat taaactcttc aggacacttt ccatctgggc agaactttga
      601 tttttttaaa tcttcaagtg ttagtgaaac cgttgaatat atactcattt tcgcactttc
      661 gttgttgtga atctttatcc gccagacatt ttctttattt tcattttcag cagaaagaaa
      721 ccagacagaa atgacagcga taaagaaaac agttaatata acaagtgatt ttttcatttc
      781 ctactcctca aacggacaca ctccttcctt ttcacaataa caatcaagat ctcgaactgc
      841 ctgtgtcgat ccttgaagaa actgataaac tatccatttt tattttcacc caagatctta
      901 taaagttgta ttaaaatatt tcagcatgtc aagggaaagc ttgcctgtac aatccatttt
      961 ttgacaaaag atgtttttta gcaatataat catttgtttt aaaactgatg aggatgaaaa
     1021 gatggctgac cagaacatca aaatagatta caaaaaatta aattcctttc tcaaagatta
     1081 catcagaatg atgaaaaagg gatggctttt tctcatcatg aacgacgatt acaatccggg
     1141 tgacagcatt ctttttaaca taaaagtcgc cggtcttgaa aaagaactta ccgcttccgg
     1201 aaatgttgtt tacaaaggcc cgaatgaaaa aggaagtgac gggatcggac ttaaattctc
     1261 ttttgatgaa agatcgtcag aacttctttc tgtaaatctt aagaagttaa ttcttgaaaa
     1321 atacggtgag acatggggcg gaagaatttg ttctttgatg gggactgatt aaatgacatc
     1381 aaattttaaa accagctccc gattctgtgt ccttgacgat gaaaagaatt ttttaagcgt
     1441 cgcaaaaggc caactttcca aatatcttcc cgagatcgaa ggattttatt cgacagaccc
     1501 tgaagaagtt tataagtttg ccgcacaaga ccgacttact ctgtatataa ttgacatgaa
     1561 cctcggcgag agcaacggca tggagatata caggaagatc gcttcaattt catatcgcgc
     1621 aagagtcatt ttcattacgg gggacatcca tttcctcgaa gatgaagata tcagaagaca
     1681 gactctatct gaaggaggga tggattttat tgaaaaaccg ataaagtggc acgagatggc
     1741 gataaaaatg aaaaaccatc tcaaccttct tgaatatcag ttccagcttg aggaaaaagt
     1801 tgaagaaaga acacagatgc tgatccacgc tgaccggctt gcaactgtcg gaacaatggt
     1861 gagttcgatc gtccacgaga taagcagtcc tctgacattc atcaaagcga atcaggaaac
     1921 atttcttttt gcatacaaca aagtaaaaga cagtctgaca gaccctgaag caaaaaaggt
     1981 ttttgaaaat tttatacttc cgggagtgaa ggattcactt tcgggaatac agcgcatcga
     2041 ggaacttctt aaatctttca ggagattcta taaaaaggaa cacaagatct cagttaacga
     2101 cattgcttca ataatcaatg aagttaaaaa ccttacctat tacggaataa aaagaggcgg
     2161 gatcaccttt tcttatcaaa taaaagatga ttcatccaac tcaataaaat gcaacagaca
     2221 ggaacttatc caggtactta caaacattgt caataacgct attgacgccc ttgacgaggt
     2281 cagacaggaa aataaagaat tgaaaatatc tgtaaaacct gaaggtcagt tcataaacat
     2341 aactgtttca aacaacggac ccgcaatacc cgaagctgtt tttgacaaaa tattcaaccc
     2401 tttctttact actaaaaaag aagacaaagg cacaggtctc gggctttcaa tcgtccatca
     2461 gataattaag ataatgggcg gcagtgttta tgcggtaaac cgttcggaag accacgatac
     2521 tgttgatttt gttttgaaac tgcctgtaaa tcaggacccg aaagacaact gaaaaacgga
     2581 gctgaaaatg aaaacggaag cgatcgttct tgccgcagga aaaggaacaa gaatgaaatc
     2641 cagcatgcca aaagtgatgc acaatgtact ttccaaaccg atgatcggct ggataatgga
     2701 atctcttgat cctgttgcag actatataaa tgtagtaaca ggtcatggaa gggatcaggt
     2761 ggaaaaatat ctatcggatg attataaatc tataaaattc tctttccagc agaaacagaa
     2821 tggaacagga ggagcagtaa aatcggcagt ccccaacatc gccggagatg catctcacat
     2881 tataatctgt acaggtgaca ctcctcttat aaaaacggat accttcagga acgccattga
     2941 acaattcaca aaaagcggat ccgatctgac agttgtaagc acgatccttg aagatgcagg
     3001 tcattacgga aggattgtca gaaacagcag aaatgaagta gaatcaatag tcgaatatct
     3061 cgatgcggat gaatcacaga tgaagataag ggaaattaac agcggaatat acatcgttga
     3121 gaaaaaattt cttgttgaag cagtcttcaa gataaagaac aacaattcaa aatatgagta
     3181 ttaccttaca gacatcgtta aaattgcaaa ccggatgaac aaaaaggtca ctgcctatgt
     3241 tgaaaacgac agcttctcac tttcaggcat caacgataag gaacaacttg cgcaagtcga
     3301 agcggaaatg cttaaaagaa tgcagtaata tctaagcttt taaaaaaata tttaaagaat
     3361 gtgcgggatt tatatattca gataagttct aattaaagct tagtactttt taccgtatcc
     3421 gcccggtttt ctttcttcgc ggggacgtgc ttcattaacg gttactgttc ttccgaaaag
     3481 ttctgtgccg ttaagtgcat tgatagccgc gagagcttca tcctggtttg gcatttcaac
     3541 aaaaccgaaa ccctttgaac ggccggtttc acgatcggtt acgaatcttg cagaatcaac
     3601 tgttccgtaa gcttcaaaag ctgctctaag gtcttcttca ctcatcttgt acgcaaggtt
     3661 tccaacgtaa atgttcatct ttctctctct caaaaaacta atactaaata accagtccat
     3721 ggaaaccgct tccatgaaca ggccgatatt aactatgaaa ttttaaaagt caagcaatat
     3781 tgcaagtttc aactaatata ggtcaattac ttgactgaaa aaccatttga ttatatgttt
     3841 taaaagattt ttctgattag ggaaattatg attttttttg agaaaatagg taatttttta
     3901 ataagggctt ttaatgatgt gtcaaagtat atcgctttca catatcagtg tcttttttac
     3961 atgtttgcgc ccccttaccg cttcggtcaa gtcgccgatc acactgaaag aatagggata
     4021 caatcaattg taatagttgc tctttcgact tttactaccg gcatgatact ttctttgcaa
     4081 cttatcaaca tcatgcggct tttcggagcc gaatcgtttg ccggtgcagg tgtagcgatc
     4141 tcaatgagtc gtgaacttgc acccgtcata acagcaatcg ttcttatcgc aaagaatggc
     4201 tcttcaatga ctgccgagat aggaagcatg aaggtaactg agcagataga tgcgatggaa
     4261 acgatggcag tgagtccggt cagatatctt gtaattccga ggatatttgc ttccctgatg
     4321 gtttttcccg cattaaccgc actcgcaaat gttgtcggaa tatacggcgg ttattttgtt
     4381 gcaaatactt ctgccatgat aaacagcact tcatatatgc acaccatgta tttctgggtc
     4441 gatcccgaag acatctggac aggacttgta aaagctttct ttttcggatt tattgttaca
     4501 acgatctgct gttatcacgg gctcaagaca aaaggaggtg caaaaggtgt cggagaagcg
     4561 accaccacgg ctgtcgttat ctcaactgtc gcaatactta tagcagatta cataatgacc
     4621 tatattttca taaacttttt cggactttag attatggaaa atattattgt aaaagtcaga
     4681 gatcttcata aatccttcag agatataaaa gttcttgaag gtgtgaatat cgacatagaa
     4741 gaaggaaagg tcacaacaat aataggtaaa agcggaaccg gaaaaagcgt tttattaaaa
     4801 aatatcattg gaataatgaa acctgaaaag ggcgaaattt tctttaaaga cattgatata
     4861 actaaacttt cagaccacaa acttcttgaa ataagaaaaa acatcggata tctttttcag
     4921 gatgcggcac tttttgattt tatgaatgtc gaagagaata ttactttccc tcttgtggaa
     4981 cagcttggaa taaaagacgg aaaattattg aaagaaaggg tggggaaact tctggaactc
     5041 gttgaacttc cgggaattga aaagaaattt ccttctgaac tttcaggcgg aatgagaaaa
     5101 agggtcggtc ttgcaagagc aatcgctgtc gaacctaaaa tagtcctttt tgacgaacct
     5161 accaccggac ttgacccaat tcttgcagaa agtatcaata cacttataat gaaggtcaac
     5221 aaggagttgg gaatgacttg tgttgtaata agtcatgaca tcgcctcaac cttcaggaga
     5281 gctgataaag tggctttgct atacgacgga acaattcaat ttttcggtga accggcaaag
     5341 gctgtgatat cagatcatcc ggtcctgaaa cagttcatca gcaattcttt cagaccgctt
     5401 gggaaggagt gattctatga ccagagccgc aaaaataggt gttttcttca ctgtctgcat
     5461 tgttatggtt tctatctata ttgtaaaatc tgctgataat tttaccggcg ggagcacacg
     5521 ggtgctctat gctctaagcg atgatgccat gggtcttctc aacgacagcg acatcaggat
     5581 tgcgggagtt gttgtcggta aaatggaaaa gatcgaactt gaggaaggaa aagcgaaaat
     5641 gacactcaac atatgggatg aagttgatgt ctacgaaaac gccagaatcg aaaaagtgat
     5701 ggaaagcatg ctcgggacat atgttcttgt tcttgatcct ggcactaagg atgcgcgaaa
     5761 acttgaagag ttcgaatata ttaagaatgt catttcttct tcaggaataa acggtgcaat
     5821 ggataaggct tccgcaatga tggaaaaagc gacagaagct atatcgatcc tcaccaacga
     5881 cgggaatcag gaaaaactta ataagataat agacgccctt tcaaggactg cggaaaacac
     5941 atcacagaca tttgaaggtt ccatgaaact tcttcttgaa acattgaaga acatgtcgga
     6001 gatcacacac aaaataaatg tgagaagtga aaatgaaatg gataagcttt caaaaatact
     6061 tgagaatact gttgcactta ccgacaaaat gaacagcatg atgaaagata aggatgaaga
     6121 aatttccgaa actctcaccg cactcagaga cagtttaaaa ctcatttccg aagaactgaa
     6181 agcatcccgt ggcgcaatgc agaatttgag agatatttcc gaagatgtta ataaaataac
     6241 cggtaaagtt gcaaacggcg aaggaaatat cggaaagatc gttaatgatg aaaaacttta
     6301 taacgacctc acaaagatat cagataaact gactgattat attgacacaa cacttggaat
     6361 gcagatacat gtcgatgcac attcagatta catggtgttt gacagagcgt ttcagacata
     6421 ttttgacata acacttcaac cgcgtgcaga ccgtttctac atgttcgggg tcgttgatga
     6481 ttcgcaagga agagttacag aaacttcaac aacatatgaa cttggtgttg atgacggatc
     6541 atctgtgcag aattatgtga taactgaaaa caagacaaaa acagactcgg aacttaaatt
     6601 caatctccag ataggaagaa atttcggtcc gatgacattc agaggcggga tcttccaaaa
     6661 taaagcaggg ctggcccttg attataatcc gtggaaattt ctttccattt cagctgaaat
     6721 atttgattcc ggcggagatg ttcctgaact tcgtgtaaaa ggacttgcaa gacctctgat
     6781 atggacaata gagcctttaa gctggatata tctgaccgtc ggcggagaaa atctgataag
     6841 cggtgaaaga g
//
LOCUS       DAOOYB010000150         6790 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_12786_length_6790_cov_11.832517, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000150 DAOOYB010000000
VERSION     DAOOYB010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6790
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12786_length_6790_cov_11.832517"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            127..807
                     /locus_tag="PL056_09155"
     CDS             127..807
                     /locus_tag="PL056_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47225.1"
                     /translation="MRSFLKIIVFSVFVSVSSSLTAQITVKPKLGEPPLKAEKFIYID
                     EKTPDYIIKNDFLKNTGFAFELKDDGDWLKRNIETITRNGVIVFINSDISKQQRKYID
                     ELAKLTGYNLKIICYKGNCESEQKWKHPLIKTTYFFKWKKNKDGEDVFFYGTGQKSKG
                     EIQSVTALNNFRKEPFIKSAVFFRDHRFGFVLIDEEERPFFIKDADIVIYFSKSEKKL
                     KEFIEKKN"
     gene            853..1590
                     /locus_tag="PL056_09160"
     CDS             853..1590
                     /locus_tag="PL056_09160"
                     /inference="COORDINATES: protein motif:HMM:NF025729.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HPM47226.1"
                     /translation="MNRALFTVLCLVFLPFISFAADAWKSVEEIEVNGTTVTKNIFVG
                     SDIMRTENESSSGKNETVIDLSSDRITIINHKTQSYQIITLSKYIEFAQQLAEEMKGK
                     GHVDPEKVIPQVTFEKIGASKVGEWDCEEWLVKVDGKPYNKVWVAPELKNLPVIKFKK
                     KFAAVMPESLAKYRSTDAKIEDNFLNSGMIVKAQKIPANKKMPVVTQTVKLVEPADIK
                     MLNFKIPADYLDKSAVENKETQPVETK"
     gene            1634..3391
                     /gene="aspS"
                     /locus_tag="PL056_09165"
     CDS             1634..3391
                     /gene="aspS"
                     /locus_tag="PL056_09165"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HPM47227.1"
                     /translation="MLRTHTCGELCLNDLGKEVVIAGWITQKRELGPILFLIISDRYG
                     MTQVAFNDPALVDTARRFTLEDVVSIKGKVIDRKENRTTKYSTGDIEIDATEITLLNN
                     SKPMPFDHRKEASDVLKLKYRYLDIRKSNIQQTLVTRSKASAAVHEYLDAEGFVEIET
                     PVLVKSTPGGARDFLVPSRISKGSFYALPQSPQVFKQILMVAGMDKYYQIVKCFRDEA
                     LRADRQPEFTQIDIEMSFGDESDVMNVTEGLLWQIFKKIKNIDIPRPFKRMSFNEAMD
                     KYGSDKPDLRFGMELMTLDGIFANSEFSAFKNAANDPKMVVKGMVLEGKSEELSKNQI
                     KKIEKEVQGDGAKALGWIKKENGELDSPLLKFFNESEILKLINMIPEKGTMFIVADKT
                     GVALTALGNLRKRLAAEYNLYDRNSYNFLWVTEFPAFEVDDESGKWVAKHHAFTDFDF
                     EAAKRGDDLSTIRSRAYDVVINGYEVGGGSIRIHNTDKQKEVFKFLNISDEEQKENFG
                     FLVEALEFGAPPHGGIALGFDRLIMLLTNQEGIRDVIAFPKTTSASCLMTGAPAPVTD
                     IQLKELNILTVPEIKDGKD"
     gene            3378..4418
                     /locus_tag="PL056_09170"
     CDS             3378..4418
                     /locus_tag="PL056_09170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPM47228.1"
                     /translation="MEKIKIPGDLTIENSEKAYLEISASISKLDSVTIDVADITLNSM
                     TKALIRKLSEEFGEKLVIDEDVRKEIFYQKEESEEEKNPRKKEPFLERFGETLIKTWE
                     IIYYFCVIMVDMLFYSFESLYNRKFVLKDDATKNAYYIGYKAIPIVSLLAFLIGLTIA
                     LQAAIQLAKMGGQGYIAMMSTMVMTKELGPLITAIIIAGRTGSSIAAEIGTMVVMEEV
                     DALKTMGIKPLKFLLVPKFWAFTLVMPILTLVADIAGIFGGFLVASVYSIPAGAFVNQ
                     MIESLEFADIFWGTIKSISFAWVILAVGSFKGLNVRGGADAVGRATTESVVVSIFLVV
                     AIDAIFSLILYT"
     gene            4421..5155
                     /locus_tag="PL056_09175"
     CDS             4421..5155
                     /locus_tag="PL056_09175"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPM47229.1"
                     /translation="MLRTENLTAGYGKNTILEDISFSIEKGERFVVLGESGCGKSTLM
                     KAIVGLNPPWTGKIFFKGEEIIHPLPEDSDFYKKIGILYQSSALMTSLSLEENVMLPI
                     IMHYPDFDTKIARDMADEKLAMVNLYNHREKYPQELSGGMKKRGALARAMVMDPDIIF
                     FDEPQAGLDPVTARDLDTLFIKLNEQLGTTYFIVTHELLSIKRTAQKVLMLADKKVLF
                     FGTFDEAMQADIFKVKRFFNVDKYSK"
     gene            5177..6694
                     /locus_tag="PL056_09180"
     CDS             5177..6694
                     /locus_tag="PL056_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HPM47230.1"
                     /translation="MVSKIGFDNEKYLKEQSEEILKRVKMFNNKLYLEFGGKILFDYH
                     AARVLPGFDPNVKMRLLQKLKDNAEIILCIYAGDIERKKMRADFGITYDVDAMRTIDD
                     FNFWGLTVRAVVITRYEGQPSAESFKNKLERRGVKVYTHSFTKGYPTDVDTIVSPEGY
                     GKNPFIETTKPIVVVTGPGPGSGKLATCLSQLYHENLNGVKAGYAKFETFPIWNLPLK
                     HLVNVAYESATCELKDVNLIDHFHLEAHKEYSVNYNRDVDAFPLLKRILEKITGEPSI
                     YKSPTDMGVNRAGFGIVDDAACVEASKQEIIRRFFRYSCEYALGLASKETVQRAEMIM
                     KDVDAKPTDRVIVDYANKSALDARNDEKGYDEIYCGSAIELKDGTIVTGNNSFLMHAS
                     SALILNAVKHLAGIPKEIHILQPSITESIGYLKKDIYKARGIGLDLEETLIALSISAT
                     FNPTAQAAMEKLKELRGCEVHLTHMPTPGDEVGLRKLGVNVTSNPEFASKHLFNS"
BASE COUNT         2154 a         1207 c         1544 g         1885 t
ORIGIN      
        1 gatactgcgg tcagtggccg tggaaaagta ggtcgtcgcc tcattttttt tgcctcaatc
       61 aggcatattt gccaaaaaac ctgtttttat gtaaaatcag tcagtttatt atcctgaggt
      121 gtattgatga gatcattttt gaaaatcatt gttttctctg tgtttgtttc agtttcttct
      181 tcattaacag cacagataac agttaagccc aagctcggag agccaccgtt gaaagcggag
      241 aaatttatat acattgatga aaaaactcct gactacataa taaaaaacga tttcctgaag
      301 aacacgggat ttgcttttga gttgaaagat gacggcgatt ggcttaagcg taacattgaa
      361 acgatcactc gtaacggtgt catagttttt attaatagcg atatttctaa acagcagaga
      421 aagtatattg atgaacttgc aaaactgacc ggttacaatc tgaaaataat ttgttacaaa
      481 ggaaattgtg aaagtgaaca gaaatggaaa catcccctaa taaaaacaac ttattttttc
      541 aagtggaaaa aaaataagga tggtgaagat gtcttttttt acggaacagg gcagaaatcc
      601 aaaggagaga tccagtcagt aaccgcatta aataatttca gaaaagaacc gtttataaaa
      661 agcgctgtgt ttttcagaga tcatagattt ggatttgttc ttattgatga agaagaaagg
      721 ccttttttta ttaaagatgc cgacatagtg atatattttt caaaaagtga gaaaaagtta
      781 aaagagttta ttgagaaaaa aaattaaaag gatagaatgt ttccgattta gttattttta
      841 agaggtaata aaatgaaccg cgcacttttt actgttttat gtttagtttt tctgccattt
      901 atttcttttg cggcagatgc atggaagtcg gtagaagaaa ttgaagtgaa tggcactact
      961 gtaacgaaga acatttttgt cggcagcgac ataatgagaa cagaaaatga atcttcaagc
     1021 ggaaagaatg aaactgtgat cgatctttca tctgatagaa ttacaataat aaaccacaaa
     1081 acacagagct atcagataat cacacttagc aaatatattg aatttgcaca gcagctcgct
     1141 gaggaaatga aaggaaaagg gcatgtcgat cctgaaaaag tcattccgca ggtaactttt
     1201 gagaagatcg gcgcatcaaa agtgggcgaa tgggactgtg aagagtggct ggtgaaggtt
     1261 gacggaaagc cgtataataa agtttgggtc gcccctgaac ttaaaaatct tccggtaata
     1321 aaattcaaga agaaatttgc agccgttatg cccgaatcgc ttgcgaaata ccgctctaca
     1381 gatgcgaaga tcgaagataa ttttctaaat tcgggaatga tcgttaaagc tcagaagatc
     1441 cctgcgaaca aaaagatgcc tgttgttacg cagacggtaa aacttgttga accggctgac
     1501 ataaagatgc tcaattttaa aattccggca gattatcttg acaagtcggc tgtcgaaaat
     1561 aaagaaacgc aaccggttga gactaaataa ctgtagatct gatcaaactc tatgaaacaa
     1621 tccggaggat agaatgctgc gtactcacac atgcggagaa ctttgtttaa atgatcttgg
     1681 gaaagaggtt gtaattgccg gctggataac acagaaaaga gaattagggc cgatactgtt
     1741 tttaataatt tcagacagat atgggatgac gcaggttgct ttcaatgatc ctgctcttgt
     1801 tgatactgca cgcagattca cgcttgaaga tgttgtcagc atcaaaggaa aagtgatcga
     1861 cagaaaagaa aaccgcacaa ccaagtattc aactggcgat atagagatcg atgcaactga
     1921 aataactctt cttaacaact caaagccgat gccttttgac cacagaaaag aggcatcaga
     1981 tgttttaaaa ctcaagtacc gctatctcga tatcagaaaa tcgaacattc agcagacact
     2041 tgtaacaagg tcaaaggcga gtgcggcagt tcatgagtat ttagatgcag aaggattcgt
     2101 tgagatcgaa accccggttc ttgtgaaaag tactccggga ggggcaaggg attttcttgt
     2161 tccaagcaga atttccaaag gttctttcta tgctcttccg caatctccgc aggtttttaa
     2221 gcagatattg atggttgcag gaatggacaa atattatcag atagttaagt gtttcaggga
     2281 tgaagcgctc agagccgaca gacaacccga gttcacccag atagatattg agatgagttt
     2341 cggggatgag tcagatgtta tgaatgtgac tgaaggtctt ctttggcaga tctttaaaaa
     2401 gatcaaaaac attgatattc cccgtccgtt taaaaggatg tcttttaatg aagcgatgga
     2461 taaatacggg tctgataagc cggatctccg ttttggaatg gagcttatga cacttgacgg
     2521 catttttgca aattcagagt tttctgcttt taaaaatgcg gcgaatgacc cgaaaatggt
     2581 tgtgaagggg atggttcttg aaggcaagtc agaagagctg agcaaaaatc agatcaaaaa
     2641 gattgaaaaa gaagttcagg gtgatggtgc aaaagctctg ggctggataa agaaggaaaa
     2701 tggcgaactt gattcaccgc ttcttaaatt ctttaatgaa tccgagatat tgaaattgat
     2761 aaacatgatt cctgaaaagg ggacgatgtt cattgttgct gataaaacag gtgttgccct
     2821 taccgctttg ggcaatttaa ggaagcggct tgcggctgaa tataatttat atgacagaaa
     2881 cagttacaat tttctgtggg tgactgaatt tcctgctttt gaagttgatg atgagagcgg
     2941 aaaatgggtt gcaaaacacc atgcttttac cgattttgat tttgaggcgg caaaaagggg
     3001 cgatgatctt tcaacaataa gatcaagggc ttatgatgtc gtgataaacg gttacgaagt
     3061 tggcggaggt tcaataagga ttcataatac tgataaacag aaagaggttt tcaaattcct
     3121 gaacatcagt gacgaggagc agaaggaaaa tttcggtttt cttgttgaag ctcttgaatt
     3181 cggtgccccg cctcacggtg gtattgctct cgggtttgac cggcttatca tgcttctgac
     3241 aaatcaggaa gggatccgtg atgtcattgc tttccctaaa acgacaagtg catcatgcct
     3301 gatgaccgga gctccggcac cagttacaga cattcagctt aaggaactaa atatattgac
     3361 agttccggaa ataaaagatg gaaaagatta agatcccggg cgacctcaca attgaaaaca
     3421 gcgaaaaagc atatctggaa atctctgcat caatctcaaa acttgattct gtcacaatag
     3481 atgtagcaga cattacactc aactcgatga caaaagcact gataagaaag ctttctgaag
     3541 agtttggtga aaaactggta attgatgaag atgtcagaaa agagatcttt tatcagaagg
     3601 aagagtccga ggaagaaaaa aatccccgta aaaaagagcc gtttcttgaa agatttggtg
     3661 aaactctcat taaaacatgg gagataatat attacttctg tgttattatg gttgacatgt
     3721 tattttattc gtttgaatcg ctttataaca gaaagttcgt tttaaaggat gatgcgacaa
     3781 agaacgccta ttatatcgga tataaagcta ttccgatcgt ttcactgctt gcttttctga
     3841 ttggtctgac cattgctctt caggcggcga tacagcttgc caagatgggt ggtcagggct
     3901 acattgcgat gatgagtacg atggtgatga caaaagaact cggccctttg atcacggcga
     3961 taatcattgc gggcagaacg ggaagttcaa ttgcggctga gatcggaacg atggttgtaa
     4021 tggaagaggt cgatgctctt aaaacaatgg gtatcaagcc gcttaaattt ctcctcgttc
     4081 caaaattctg ggcgttcacg cttgttatgc cgattttgac gcttgtagct gatattgcag
     4141 gaatttttgg tggttttctt gttgcttcgg tttacagtat tcctgccgga gcttttgtaa
     4201 accagatgat agaatccctt gaatttgctg atattttctg gggaactatc aaaagtatct
     4261 cttttgcatg ggttattctt gcagtcggca gtttcaaagg gcttaatgtc cgcggcggag
     4321 cagatgcggt tggaagagcg acaacagaat cggttgttgt ctcaatattc ctcgttgttg
     4381 caattgatgc aattttttca ctgatattat atacttaaca atgcttagaa ctgaaaacct
     4441 cacagcggga tacgggaaga acacgatcct tgaagatatt tctttttcaa ttgagaaagg
     4501 ggaaagattt gtcgttcttg gtgaatcggg ttgcggaaaa tcaactttga tgaaggcgat
     4561 cgttggactc aatccgccat ggaccggaaa aatatttttt aagggtgaag agatcattca
     4621 tccgcttcct gaagattctg atttttataa gaagatcggg atcttatatc aaagttcggc
     4681 acttatgaca tcgctttcgc ttgaagagaa tgttatgctt ccgatcataa tgcattaccc
     4741 tgattttgac acaaagatcg cccgcgatat ggctgatgaa aagcttgcga tggtcaatct
     4801 ttataatcac agggaaaaat atccgcagga gctttcgggg ggtatgaaaa aaaggggtgc
     4861 tcttgcaagg gcgatggtta tggaccctga cataatattc tttgatgaac cgcaggcggg
     4921 gcttgatcct gttacggcaa gagaccttga cacactgttc attaagctca atgaacagct
     4981 cggaaccacc tatttcattg taacccatga acttctttca ataaaaagga cagcacagaa
     5041 agtgttgatg cttgcggata aaaaagttct tttctttgga acttttgatg aagcgatgca
     5101 ggccgatatt tttaaagtta aaagattttt caatgttgat aaatatagta aataattaaa
     5161 gaaaacggag taaaagatgg tatcaaagat cggttttgac aatgaaaagt atctgaaaga
     5221 acagtctgaa gagatcttaa aaagggttaa gatgttcaac aacaagctct atcttgagtt
     5281 tggcggaaag atcctttttg attaccatgc ggcgcgtgtt cttccgggtt ttgatccgaa
     5341 tgtgaagatg cgtcttctgc agaaacttaa agacaatgcc gagataattc tgtgtattta
     5401 tgccggcgac atcgaaagaa agaagatgcg tgccgatttc gggataactt atgatgttga
     5461 tgcgatgagg acgattgatg atttcaattt ctgggggctg acggtcagag cggttgtcat
     5521 aacccgttac gaaggtcagc cgagtgctga atcgttcaag aacaagctcg aaagaagagg
     5581 tgtgaaggtt tatacccaca gttttacaaa aggttatccg acagatgttg acacgatcgt
     5641 aagtcctgaa ggttacggca aaaatccgtt cattgaaacg actaaaccga tagttgtcgt
     5701 cacaggtccc ggtcccggca gcggaaagct tgcaacatgt ttaagtcagc tttatcacga
     5761 aaatctaaac ggagtgaagg cgggatacgc aaaatttgag acttttccaa tctggaatct
     5821 gcctcttaag caccttgtca atgttgccta tgaatcggct acatgcgaac tgaaagatgt
     5881 taatctgatt gaccattttc atcttgaagc acacaaggaa tattcagtca actacaaccg
     5941 tgatgttgat gcgtttcccc ttttgaaaag gattcttgaa aagatcacag gggagccgtc
     6001 aatttataaa tcaccgactg acatgggtgt aaaccgtgcc ggctttggaa tagttgatga
     6061 tgccgcatgt gttgaagcgt caaagcagga gatcataaga aggtttttcc gttattcatg
     6121 cgaatatgca ctcgggcttg caagcaagga gactgttcag cgtgccgaaa tgataatgaa
     6181 agatgttgat gcaaagccaa ccgatagagt gatcgttgat tacgctaata aatcggctct
     6241 tgatgcaaga aatgatgaaa aaggatacga tgaaatatac tgcggatcgg cgatagagct
     6301 taaagacggc acaattgtta caggcaacaa ctcatttctc atgcacgcct cttcagcact
     6361 tattcttaac gcagtgaaac atcttgcagg aattcccaag gaaattcata tcttacagcc
     6421 aagcataaca gaatccatcg gatatctcaa aaaagacatc tataaagcaa gaggaatcgg
     6481 actcgacctt gaagaaacac tcatcgcact ctccatcagt gccacattca accccacagc
     6541 acaagccgca atggaaaaac tcaaggaact cagaggatgc gaagtccatc ttacccacat
     6601 gcctacaccc ggcgatgaag tcggacttag aaaactcggc gtcaatgtca ccagcaaccc
     6661 cgaattcgcc agcaaacatt tgtttaattc gtagggtatt aaccacctaa tttcgcaagt
     6721 atttctgttg atttgatatc catttttgca aatccgaacc attttctgcc gaggtctttg
     6781 agtgatgctc
//
LOCUS       DAOOYB010000151         6774 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_12820_length_6774_cov_8.781962, whole genome shotgun sequence.
ACCESSION   DAOOYB010000151 DAOOYB010000000
VERSION     DAOOYB010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6774
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12820_length_6774_cov_8.781962"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1581
                     /locus_tag="PL056_09185"
     CDS             <1..1581
                     /locus_tag="PL056_09185"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HPM47231.1"
                     /translation="DYNIAVTNAERNSEDRNRDGLCNYSDYRPGLVNTGGVARVKTVI
                     DRIRASHGDIPVMHLDSGDAFQGAPQFNLYKGELEMLAIQKLGIDAMAIGNHEFDNGT
                     SALVSAYKKSGGFPLLASNYLFDWDGHKGLMDITSPYLILMRGSLRIGIIGVGNDSSL
                     TSIYQIGGSIGFNVIDPIESAQTYVNVVRPLVDLVIILSHQGLEGDYEIAEKVEGVDL
                     VMGGHHHVVLDPVKVIEGPDGRDVLVVHSGVNYKILGELELAVQNKRVVWHNYKTHAI
                     NESVPEHGDMINLLKPYVQGLEFSQYLQRIVGRATSTIVRNDPSGGDSPLGNIVTDAM
                     MNHELSRSQFCVTNSMGIRADIPTGDITLEKLYEVFPFENSITTMYLSGNELKTLFDF
                     VARKSASRGCKTQIQVAGIGVEIDCSPDEELQKKYGSYSLTKCLTIGNVEVIKDYEVV
                     LPHMLFKMATNDYMGKGGSGFYMLEYNTTRLDTSVSLRDAVSDFLSEKEVIDPLDYSL
                     NQTGADECGQGKRIKMIN"
     gene            1590..2759
                     /locus_tag="PL056_09190"
     CDS             1590..2759
                     /locus_tag="PL056_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47232.1"
                     /translation="MKTQIMLITIISIMISSGCYGLKGNPEPENSGLAAFKVELADNF
                     DPGTPEKRLEKAERYDLSINISALDYNREVLKDYNGTVEIALQYGKITSVARADLKNG
                     VLTDFPVEMRYILGKEKIVVHELIIDEEKSSKDSVTYKRSGKLGVSETIYPPLASISE
                     IQGNNSGSKGFDSKYNNRNLNLKGKTMVVTAVIEGGFYLAEVGVEDYGAIYLYTYSTP
                     YVDDPDVGYSLPIGSIIEEANGSVFEFFGFTEMSFPTFKPKYKTVDGKKRIVVDESLV
                     PEPFDISDLLSSNSDMEKKEASIVTVKNVTVDWFNEHDSSFVEYGQFPLKTESGGSIM
                     AQTLYTAPSFDPLKERDAETKRKFNFTGALKQHTSARPSTWILVPIDSNDIEVIE"
     gene            2775..4604
                     /locus_tag="PL056_09195"
     CDS             2775..4604
                     /locus_tag="PL056_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47233.1"
                     /translation="MKKILFVLIIMSVFIISAQEEEIKPAEETAKPEYVIPQNVEEVK
                     TEELKKEEEPQKETVTMSKEEIEQLVKKMVAEELAKKDAEKKKEEPEKKEETAEKPNK
                     LNERDGLIAPNYSKSSVTFVMGDENLRDNSQYSPKWDIGNRYEYESFAQRIYGYSNIA
                     KSSSQFTLFHEEEGFIKNLTARISLSFAMYNSMMPSNRNVSTSFAENKTFLEIDYRHP
                     STNRFKLTFYPYNAESIAIGNFRGLRWGSNRMVWPQQQSGQTAVPGFQALYGYGDISV
                     YFGFKANAQQETDVLAGELAPMETTYGFFGGVSYSNKDLGLKGHLQGAFIDKGDNVNI
                     EEAILADPDDDKILSYGFDAFAEYNYGSQLGDPIGINSFLDGSWLKPDYTSKFAFRVK
                     GEYLFQNQRLQNADYMSIQNVGNIEPITDNFFAHGAAFELGGRFKGIRAMFMYSYRDL
                     PFMVFDAPGVVPYQTISSQADQSPEHLFTISADYHIWDLWFGISYGYKIPATYTVYDA
                     NGQKNVTVIKERISSDAVSTAFDRSREVLPAGRDPLDMMFIKLNVKYGFSDTVTAMLE
                     YSFTQDHNRQKMVATEDGPYVGEFDVIEVMDIHGLFFLVEGRF"
     gene            complement(4601..6403)
                     /locus_tag="PL056_09200"
     CDS             complement(4601..6403)
                     /locus_tag="PL056_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47234.1"
                     /translation="MKNMLVVILILFLSSSCEISTTARKDLTDDSDTISHNDEQSHSD
                     SDETTGDSDPDDESKEDDGSETDNESETDDESEMVDNGSETDDQSEADDESETDDEIQ
                     LPDNDENFPDDNEIPDKDNVGKIAVTVGTYNLENFFDTECDTGTDIYGDCYDFFVLSV
                     SEFNAKLAAVETSIRSINSDIQILVEIEDNTTGNALLNEMSDIYDEVYITPRNAGGQN
                     VGVLTKGTIEKVVNHGNETDFVREFAEIHISYLGHNIIVFPAHFKAKSNDDPARRLRE
                     ATKAAEIINEVADMYPDSLIVLAGDLNDEPGSDPLNALDSASLLRTASDLTINEQCTY
                     NYNGCSKIDHIYLSLDGAGEYVTHSSEVIKDGGCGSGPYCLGDSDHAALKAEFYLGED
                     ETVPDEDELPDDDVMPEFSTIYDIKTGTIPSSTDVLVKGIVTAVDENSFFIQTPASDY
                     HPTLQEKYSAVFVYISSSEPPSFSIPDIGDIVEVTGTTGVYYDQTQIYSVTDVSIKGS
                     ASVPPVLNISSSSVRSAEYEAVFVDVGNVTVTEIGSYGDFIVTGGLYVDDDIYHILPF
                     PEVGEVYNLKGVIKYSFEQYRLLPRSSSDVEIIY"
     gene            complement(6539..>6774)
                     /locus_tag="PL056_09205"
     CDS             complement(6539..>6774)
                     /locus_tag="PL056_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPM47235.1"
                     /translation="EMMPSLGKKIYNEGRQEGREEGLQIGIEKGIEKGAFAKAVETCK
                     KALKKGFDINTIIELTGLPVEKIKEIQKTLQTD"
BASE COUNT         2052 a         1387 c         1414 g         1921 t
ORIGIN      
        1 gattacaaca ttgccgtaac aaacgctgaa aggaatagtg aagacagaaa cagagacggg
       61 ctttgcaact attccgatta ccgtcccggt cttgtaaata ccggcggtgt ggcaagagtc
      121 aaaacggtca ttgacagaat aagggcttct cacggtgaca ttcccgtgat gcatcttgac
      181 agcggagatg cttttcaggg tgctccccag ttcaatctat acaaagggga actcgaaatg
      241 ctcgccatcc agaaactcgg cattgatgca atggcgatag gaaaccacga gttcgacaac
      301 ggaacttcag cccttgtttc ggcctataaa aaatcgggtg gatttcccct tcttgcgtct
      361 aattatctct ttgactggga tggacacaag gggctgatgg atatcacttc gccttatctt
      421 attttgatga gaggttctct tcgaatcgga ataatcggtg tcggtaatga ctcatcgctc
      481 acttcgattt atcagatagg cggaagcatc gggttcaatg tcattgatcc gatcgaatct
      541 gctcagactt atgtaaatgt tgtccgtcct cttgtcgatc ttgtcataat cctttcgcat
      601 cagggtcttg aaggagatta cgaaattgct gaaaaagttg aaggggttga tcttgtcatg
      661 ggcggtcacc accatgtcgt tctcgatcct gttaaagtga tcgaaggtcc tgacggcaga
      721 gatgttctcg tggttcacag cggtgttaat tacaaaatac tgggcgaact tgaactcgcc
      781 gttcagaata aacgggtcgt ttggcacaat tataaaactc atgcgataaa cgaatctgtt
      841 cctgaacacg gagacatgat aaacctgctt aaaccctatg tgcagggtct tgaattttcg
      901 cagtatcttc agcggatagt cggtcgtgcc acttcaacga tagttcgtaa cgatccaagc
      961 ggcggtgaca gtcctcttgg aaatatagtc actgatgcga tgatgaacca cgagctttca
     1021 aggtcgcagt tctgtgtgac caactcaatg ggaataaggg cagacatccc gacaggcgat
     1081 atcacacttg aaaagcttta cgaagttttc ccgtttgaga acagcataac gacaatgtat
     1141 ttaagcggca acgaattgaa aactcttttt gattttgttg ccaggaaatc tgcatcgaga
     1201 ggttgcaaaa cccagataca ggttgcaggc atcggtgtgg aaattgactg cagtcctgat
     1261 gaagaactgc agaaaaaata cgggtcatac tcacttacga aatgtctgac tatcggaaat
     1321 gttgaagtca ttaaagatta tgaggtcgtc cttccccaca tgctctttaa aatggctact
     1381 aacgactaca tgggcaaggg tggaagcggt ttttatatgc ttgaatacaa cactacaagg
     1441 cttgacactt cagtttcatt aagagatgcg gtaagcgatt ttctaagtga aaaagaggtg
     1501 atagatccac ttgattattc tttgaaccag accggagcgg atgagtgcgg tcaggggaaa
     1561 agaataaaaa tgataaattg aggtggccga tgaaaacaca aattatgctg atcacgatca
     1621 tttcaattat gatttcatcc ggttgttacg gtctgaaggg aaatccggaa ccggaaaaca
     1681 gcggacttgc cgcattcaaa gtagaacttg ccgataactt tgatccggga actcctgaaa
     1741 agcggctcga aaaagccgaa agatatgatc tgtcaataaa catttccgct cttgactaca
     1801 acagagaagt tctgaaagat tacaacggga ctgttgagat cgcccttcag tatggaaaga
     1861 taacatcagt ggcaagagca gatcttaaga atggagtttt gactgatttc cctgttgaaa
     1921 tgcgatatat tctcggaaag gaaaagatcg ttgttcacga actgattatt gacgaagaaa
     1981 aaagttcaaa agattcagtc acttacaaaa gatccgggaa actcggtgtt tctgaaacca
     2041 tttatcctcc tcttgcctca atatcagaaa ttcaggggaa caactcgggt tcaaagggtt
     2101 ttgactcgaa atataacaac aggaacctga atctgaaagg gaaaacaatg gtggtcaccg
     2161 cagttattga aggcggtttc tatcttgcgg aagttggtgt tgaagattac ggtgcgatct
     2221 atctctacac ctactccact ccttatgttg atgatcctga tgtgggctat tcccttccta
     2281 tcggctcgat aattgaagaa gccaacggtt ctgtctttga atttttcgga tttaccgaaa
     2341 tgagtttccc gacattcaaa ccgaaatata aaacagttga cgggaaaaaa cggatcgttg
     2401 tcgatgaatc gcttgttcct gagccgtttg acatttctga cctcctatct tcaaattcag
     2461 acatggagaa aaaggaggcc tcgatcgtaa ctgtgaaaaa tgtaactgta gactggttca
     2521 atgaacacga ttccagcttt gtagaatacg gtcagttccc tttaaaaact gagtcaggcg
     2581 gatctatcat ggctcagact ctgtacactg ccccctcttt tgatcctctg aaggaaagag
     2641 atgcagaaac aaagagaaag tttaatttta ccggtgccct gaagcagcat acaagtgcaa
     2701 gaccgtcaac atggatactc gttccgatag atagtaatga tattgaagtt attgaataaa
     2761 tttggagaga tgttatgaaa aagatcttat ttgtgctgat tatcatgtcg gttttcatca
     2821 tttccgcaca ggaagaggaa ataaaacccg ctgaggaaac tgcaaagccg gaatatgtga
     2881 tccctcagaa tgttgaagag gttaaaaccg aagaacttaa aaaagaagaa gaaccgcaga
     2941 aagagacagt aaccatgtca aaagaagaga tcgagcaact cgttaaaaaa atggttgccg
     3001 aagaacttgc aaaaaaagat gctgaaaaaa agaaggaaga gccggaaaag aaagaagaaa
     3061 cggctgaaaa acccaataag cttaatgaaa gggacggact tattgcccct aattattcaa
     3121 aaagcagtgt aacttttgtc atgggggatg aaaatcttcg tgacaacagt cagtattctc
     3181 caaaatggga tataggaaac agatacgaat atgaaagctt tgctcaaaga atttacggat
     3241 attcaaatat tgcaaaaagt tcttcacagt tcaccctttt tcacgaagaa gagggcttta
     3301 tcaaaaatct tacggcaaga atttctcttt cttttgcgat gtataactca atgatgcctt
     3361 caaataggaa tgtttccaca agttttgccg aaaataaaac attcctcgaa attgactaca
     3421 gacatccatc cacaaacaga tttaaactca cattctatcc ctataatgct gaaagtattg
     3481 cgattggaaa tttcagagga cttcgatggg gctccaacag aatggtgtgg cctcagcaac
     3541 aatcgggaca gactgctgtt ccgggttttc aggcacttta cggatatggt gacatttcag
     3601 tgtatttcgg atttaaagcc aatgcacagc aggaaacaga tgtccttgcc ggagaacttg
     3661 ctccaatgga gacgacatac ggatttttcg gcggagtgag ctacagcaac aaagatctcg
     3721 gattaaaagg acatcttcag ggagcgttca ttgataaagg tgataatgtc aatatagaag
     3781 aggcaatact cgcagatcct gatgatgaca aaatactttc atacggattt gatgcctttg
     3841 ctgagtataa ttacggttca cagcttggag atcctatcgg gattaactca tttttagacg
     3901 gatcatggct taaaccggat tacacctcaa aatttgcttt cagagttaag ggagaatatc
     3961 ttttccagaa ccagcgactt cagaatgcag actacatgtc aatccagaat gtaggaaata
     4021 ttgaaccgat caccgacaat ttctttgccc acggagccgc ttttgagctt ggtggaaggt
     4081 ttaaagggat cagagctatg ttcatgtatt cataccgtga tcttcccttc atggtgtttg
     4141 atgctcccgg agttgttcct tatcagacta tctcttctca agcagatcag tcacctgaac
     4201 atctttttac aatttcagca gattatcata tatgggactt atggttcgga atcagttacg
     4261 gatacaagat tcctgcaact tacacagttt atgatgcaaa cggacagaaa aatgtaactg
     4321 tcataaaaga aagaatttct tccgatgccg tttccacagc tttcgaccgt tcaagagaag
     4381 ttcttcctgc cggcagagac ccgcttgaca tgatgttcat aaagctcaat gtaaagtatg
     4441 ggttcagcga tacagtcaca gcaatgcttg aatattcatt tactcaggac cataaccgtc
     4501 agaaaatggt tgcaactgaa gatggtccct atgttggaga atttgatgta attgaagtca
     4561 tggatattca cggattattt ttccttgttg aaggaagatt ctaataaata atctctacat
     4621 ctgaacttga tcttggaaga agccggtact gctcaaaaga atattttata actcctttta
     4681 aattataaac ttctccaact tctgggaacg gcagaatgtg ataaatgtca tcatcaacat
     4741 aaagtccgcc tgtcacaata aaatctccgt atgaaccgat ctctgtaact gtcacattcc
     4801 ccacatccac aaaaaccgcc tcgtattcag cacttctgac agatgacgaa gaaatattaa
     4861 gaaccggagg cacagatgca cttcctttga tcgaaacatc ggtcactgaa tatatctggg
     4921 tctgatcata ataaacgcca gtagtgcctg tcacttcaac gatatctcca atatccggga
     4981 ttgaaaatga agggggctct gaagaagaga tatagacaaa taccgcactg tatttttctt
     5041 gaagcgtcgg gtgataatca cttgcaggag tctgaataaa gaaggaattc tcatcaacag
     5101 cggttacaat tcctttgacc agaacatctg tggatgaagg gattgtcccg gttttaatgt
     5161 catatatcgt ggaaaactca ggcataacat catcatccgg caattcatct tcatcaggaa
     5221 ctgtttcatc ctctccgaga tagaattccg ctttcaatgc ggcatgatct gaatctccaa
     5281 gacagtacgg tccgcttccg cagccgccgt ctttaattac ttcagatgaa tgagttacat
     5341 attcccctgc cccatcaaga gaaaggtaaa tatgatcaat tttactgcat ccgttgtagt
     5401 tatatgtgca ctgttcattt atggtaagat ctgaagcagt ccttaaaagt gacgccgaat
     5461 cgagggcatt cagcggatcc gaacctggct cgtcattaag atctcctgca agaactatca
     5521 atgaatccgg atacatatct gccacttcgt taattatttc agccgctttt gtcgcctctc
     5581 taagccgtct tgcaggatcg tcatttgatt tggctttaaa gtgtgcaggg aaaactataa
     5641 tgttatgacc aagataagaa atatgtattt cagcgaattc tctaacgaaa tcagtttcat
     5701 tgccgtgatt taccaccttt tctatagttc cttttgtaag aactccgaca ttctgcccgc
     5761 ctgcattgcg gggcgtgata taaacttcat cataaatatc gctcatttca tttaaaagtg
     5821 catttcctgt cgtattgtct tcgatctcga caaggatctg aatgtcagaa tttatacttc
     5881 tgatgcttgt ctcaactgca gcgagctttg cattgaattc agaaaccgaa aggacaaaga
     5941 aatcataaca gtcgccataa atatcagttc cggtatcaca ttcagtatca aagaagttct
     6001 caaggttgta tgtcccgaca gtgaccgcga tctttccgac attatcctta tcaggaattt
     6061 cgttgtcatc aggaaaattc tcatcattat caggaagttg gatttcatca tccgtttcag
     6121 attcatcatc cgcttcagat tgatcatcag tttcagatcc gttatccacc atttcagatt
     6181 catcatccgt ctcagattcg ttatcagttt cagatccgtc atcttcctta gattcgtcat
     6241 ccggatcaga atcacccgtt gtttcatcag aatcagaatg tgactgctca tcattatgag
     6301 aaatagtatc tgaatcgtct gtcaaatctt ttcgcgcagt tgtagatatc tcacatgagg
     6361 acgacaaaaa aagaatcagt attacgacca gcatattttt cataaagacc tccttccgct
     6421 tgagaacagc ttcatattat ctgttaaatt taaaattgca aaaagggggg ggtaaccgca
     6481 aggaaaatat ctttcgggaa taatcttact gatagatttt gtgttctata atgaaaattt
     6541 aatctgtctg aagagttttc tgaatttctt tgattttttc aacaggaaga cctgtcaatt
     6601 caatgatggt gtttatgtca aatcctttct tcaatgcctt tttacaagtt tcaacagctt
     6661 tggcaaaagc acccttctca attcccttct caattccgat ctggagtcct tcttctcttc
     6721 cttcctgtct gccttcgttg tatattttct ttccaagtga cggcatcatt tcct
//
LOCUS       DAOOYB010000152         6773 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_12824_length_6773_cov_12.629652, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000152 DAOOYB010000000
VERSION     DAOOYB010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6773
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12824_length_6773_cov_12.629652"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="PL056_09210"
     CDS             complement(<1..330)
                     /locus_tag="PL056_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47236.1"
                     /translation="MSTIDFIGVETKKNISEFQALAVTVMNAPASVEADAQALYNAAL
                     EQPTVVVTDQTLPKGAEMGLNDAKVLVNLTGEDVQTASWAVRIVPTVAEAVTNPGLAD
                     ERGELIGI"
     gene            517..1050
                     /locus_tag="PL056_09215"
     CDS             517..1050
                     /locus_tag="PL056_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47237.1"
                     /translation="MKRIFAAFFVLLFFQFVFADKVSVLLKNGKSVSGTVTKIEAGSI
                     DGKNVVEVSSINAAQGVNEILVQIKDIKEIVFKSSDDVSCFEDGRFVPVRKYCSMKSL
                     YHVILKTPSKDKTPIEISDERMFIFSIEGAKEPVKVGFYKVLVSNENREDKVDYPDLE
                     REVMEFSRNNIKKLSFN"
     gene            1063..1383
                     /gene="trxA"
                     /locus_tag="PL056_09220"
     CDS             1063..1383
                     /gene="trxA"
                     /locus_tag="PL056_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012855975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HPM47238.1"
                     /translation="MLSVSLSNFASEVEEFKGLVIIDFWAPWCGPCRMLGPIFEELAG
                     DYADNSSIKFVKINTDEDPDLARKFQIRGIPTIKFIKNGKEVDQQVGAVPKEVIKEFI
                     SKHI"
     gene            1480..5754
                     /locus_tag="PL056_09225"
     CDS             1480..5754
                     /locus_tag="PL056_09225"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HPM47239.1"
                     /translation="MESKDLFFRTGIDAGSTTLKIAVLDESGKVVFSSYLRHNTDIKG
                     TLLTVLDNMKKKIGDVVTKVCVTGSAGIGISERVGLKFIQEVVASAEAVSGLFGKIGT
                     LLDIGGEDSKMIIFDESGNADIRMNGNCAGGTGAFIDQMASLMSVQVEELSNLAADHT
                     KIYPVASRCGVFAKTDVQNLLSREIPKGDIAASIFNAVVTQVMNTLARGSSIKPPLLF
                     SGGPLAFIPFLRDLFLKRIGLTESDAVIPEKPELFPAMGSAYYAKDNELSMTVSELIS
                     LITSSKESKTAGGGRLDPLFKSEEYFRKWYENRYMKVTRAVPELSENSDCFLGIDAGS
                     TTTKIVVINKDGEIMAEYYRGNGGDHIVSVRSGLTEIQKKLASEGIRYHVKAVAVTGY
                     GEDLVKSVFSADFGIVETLAHLKSAQKISNGNVDFILDIGGQDMKAMFVENGNVTNIE
                     INEACSSGCGSFIQTFAVSLGLSVEEFANKGCSSKAPCDLGSRCTVFMNSKVKQALKE
                     GAGIEDISAGLAFSVVRNSLYKVLKLKDMDEIGQNVILQGGTFKNPCVQRAFEIITGK
                     KVVVPDIAGVMGAYGAALNALEKWNENRDMVSEFTGFFSCTGELALKMKKTLIECKGC
                     ENICSVTKMDMGNGKVFYAGNNCEKIFFSGGGNVQTQCFDGVSWKRNLLFDRDSCPES
                     EALITVGIPRALNIYENYPFWHTLFTRCGIRIILSDPSTVQLYESGSGSVMSDSICFP
                     AKLMHGHIFNLINRKVDRIFYPMVVYEDKSGCWQNSFNCPIVSGYSDVVKSSINPENN
                     YGIPLDTLAVSFKDNNLLEKKLKSYFTKTFGVSHYRFRKAFAEAMKEKDNFKVILRQK
                     SDEIITKAKQEGRKVVMLIGRPYHIDPLINHKIPEMITVFGFDVITEDSVSQTCGTAV
                     KVLDQWEYSNRLYSAAWYVAENDAVIPVQLNSFGCGPDAIATDEMKEILKSYGKNLTT
                     IRIDEIVSPGSTKLRLRTLFESMERGGNRTKFLRKELAVLKEEDLKKQILIPFFADFY
                     SPFVEHGLRAAGINVVSLPKPDAESLKEGLRYANNEICYPAIIIVGDIIKALKSGKYD
                     LENTVIGITQTGGQCRASNYFSMIRKAMAAAGFDRVPVVSFSTTNKAVTKQPEFKIRK
                     IPFINWGLKAILLADAVSRMYNAVAAREKTKGSALKLANSLIAEWNLTSFDVNRKEMF
                     RFLKKAINSFNSIEIDHDPRPKIGVVGEIYVKYSAFANHDVLEWLMNHGIEPVMPDLV
                     DFFVQEIINVKINSRENLSRNDMFSLFLPLLEKHFTKFHEEVGNEMKRFKFSYPYYGV
                     RDLAGKASKILSLVNQFGEGWLIAAEIASFAENGINDVLCLQPFGCIANQVVAKGVEK
                     KMKDIYPGLDILFIDLDSDTSPANMMNRVHFLVRNAKNHKIRRSHE"
     gene            5747..6328
                     /locus_tag="PL056_09230"
     CDS             5747..6328
                     /locus_tag="PL056_09230"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPM47240.1"
                     /translation="MNDLREKIISEAGKLFYTYGFKRVSMDEIASKSKISKKTLYTAF
                     GSKNEIMKIFLERKVDQFADDVDALFDTSLPVEDTFREYIGLFKRLSFSISTPMVSDM
                     KYMPEMFDIVDKKRRKMLLKYKVILDKAKEEGIISPDLNTDVFMEILMNTMSSTIIPA
                     KLVELNISPGDLIGQIITIFFFGVFRKERGIID"
     gene            complement(6340..6660)
                     /locus_tag="PL056_09235"
     CDS             complement(6340..6660)
                     /locus_tag="PL056_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47241.1"
                     /translation="MHDFFLYDWHTDHPEKGDNHGKEHPKTALRNAKKHFSLNKIEED
                     IIINHMWPYTKTFPSTKEALVVLAADKYFASKELFKEAESVLGKNLEILVSKMKKNRK
                     KQNN"
BASE COUNT         2088 a         1155 c         1502 g         2028 t
ORIGIN      
        1 tatgccgata agttcacctc tttcatcggc aagcccggga tttgtcacag cttcggctac
       61 ggtcgggaca atccttactg cccagctggc ggtctgaaca tcttcacctg tgagattgac
      121 cagtactttc gcatcgttaa gtcccatttc ggcccctttg ggaagtgtct ggtccgtaac
      181 aactacggtc ggctgttcca gtgccgcatt gtaaagtgcc tgtgcgtcag cctctacaga
      241 tgcaggtgcg ttcatgaccg tgactgcaag tgcctgaaac tctgaaatgt tcttttttgt
      301 ctcaacaccg atgaaatcaa tagtgctcat tctgttaccc cttaatattg ctggataaaa
      361 tttaaaaagc ctcagaatta tacaaaaaag tcttttaata tcaaaataat tagtaaaaaa
      421 attcactatt tgtttaatat tttgtgtcaa accttgccat ttctatcaat agacttataa
      481 tcaatctcgt ttatttaatg ccttaaggag gtcattatga agagaatatt tgcagctttt
      541 tttgtattat tgttttttca gtttgttttt gctgacaaag tttcggttct gcttaaaaac
      601 gggaagagtg tttcaggaac ggtgacaaag atagaggccg gcagcataga tggaaaaaat
      661 gttgttgaag tttcatcaat aaatgctgct cagggtgtga atgagatcct tgtccagata
      721 aaggatatca aagagatagt tttcaagtca agtgatgatg tttcatgttt cgaagacgga
      781 aggtttgtcc ctgttagaaa atactgctca atgaaatctc tctatcatgt tattctgaaa
      841 acaccgtcaa aagataaaac acccattgaa atttctgatg aaaggatgtt cattttttct
      901 attgaaggag caaaagagcc ggtaaaagta ggcttttaca aagttcttgt aagcaatgag
      961 aaccgtgaag ataaagtgga ttatcctgat cttgaaagag aagtgatgga attcagcaga
     1021 aacaacataa agaaattatc attcaattaa ccggaggaac cgatgctttc agtcagtttg
     1081 tctaattttg catctgaagt cgaagaattc aaaggacttg tcataattga tttctgggca
     1141 ccgtggtgcg gaccatgcag aatgctggga ccgatatttg aagaacttgc cggagattac
     1201 gcagacaaca gctcaataaa gtttgtaaag atcaatactg atgaagatcc tgatctggca
     1261 agaaagttcc agataagggg aattcctaca ataaaattca ttaagaacgg aaaagaagtc
     1321 gatcagcagg tcggtgcagt gccgaaagaa gttataaagg aattcatttc taaacatatt
     1381 taagctactt gaaaaactat cttttctgat cttgacaaac agaaatattt tgcaacaatg
     1441 gaaactcaaa tcgttttttg aagtttccgg aggtttccaa tggaaagtaa agacctgttt
     1501 ttcagaaccg gaattgatgc cggttctaca actttgaaaa ttgctgttct cgatgagtcg
     1561 gggaaagtgg ttttttccag ttatttacgg cataacactg acatcaaagg aactctgctt
     1621 actgttcttg acaacatgaa gaagaaaata ggcgatgtcg ttacgaaagt atgcgtgaca
     1681 ggttctgctg ggataggtat ttcagaaaga gtcggcctga aatttattca ggaggttgtt
     1741 gcaagcgccg aagctgtgtc aggattgttc ggcaaaatcg ggaccttact cgatatcggc
     1801 ggagaagata gcaaaatgat aattttcgat gaaagcggaa atgccgatat aagaatgaac
     1861 ggcaactgcg caggcggcac aggagctttt atcgatcaga tggcttctct gatgagtgtt
     1921 caggttgagg aactcagcaa tcttgccgcg gatcatacaa aaatttatcc ggttgcaagc
     1981 agatgcgggg tttttgcaaa aaccgatgtg cagaatctgc tgagccgcga aattccgaaa
     2041 ggcgatatag ctgcttcaat ttttaatgct gttgtaactc aggttatgaa tactcttgcg
     2101 agaggaagca gtatcaagcc gccactgctt ttttcaggtg gtccgctcgc ttttattccg
     2161 tttcttcgtg atctttttct taaaagaatc gggttgactg aatcagatgc ggttattccg
     2221 gaaaagccgg agctttttcc tgccatggga agcgcttact acgcaaagga taatgaactt
     2281 tccatgacag tttctgaact tatttcactt atcacttcga gtaaggaaag caaaactgcc
     2341 ggcggtggga ggctggatcc gctttttaaa tctgaggaat atttcagaaa atggtatgaa
     2401 aaccgttata tgaaagtcac tcgtgctgtt cctgaactat ctgaaaacag tgattgtttt
     2461 ttgggaatag atgcaggatc gacgacaact aaaatcgttg tgataaacaa agacggcgaa
     2521 atcatggccg agtattacag gggtaatggc ggggatcata ttgtttcagt caggtcagga
     2581 cttactgaaa tccagaaaaa gcttgcttcg gaaggaatca gatatcatgt aaaggctgtt
     2641 gccgtcactg gttacggtga agatctcgta aagtccgttt tttcagctga tttcgggatt
     2701 gtcgaaacgc ttgctcatct taagtccgca cagaaaatat caaatggtaa tgtggatttc
     2761 attcttgata tcggcggaca ggatatgaaa gcgatgtttg ttgaaaacgg aaatgtcacg
     2821 aatatagaaa taaatgaggc atgttcatca ggttgcggtt cttttattca gacatttgcg
     2881 gtaagtctgg gacttagtgt cgaagaattt gccaataaag ggtgttcatc caaagctcca
     2941 tgcgatcttg gatcaagatg tacggttttt atgaattcca aggttaaaca ggcacttaaa
     3001 gaaggtgccg gaattgaaga tataagtgcg ggtcttgctt tttcggttgt aaggaactct
     3061 ctttacaagg ttctgaaact caaagatatg gatgaaatcg gacagaatgt cattcttcag
     3121 ggaggaacat ttaaaaatcc gtgtgttcag agggcatttg aaattatcac tggaaaaaaa
     3181 gttgttgtgc ctgatatcgc aggagtcatg ggagcttacg gagcggcatt gaatgcgctt
     3241 gaaaagtgga atgaaaacag ggatatggtg tctgaattta ccggattttt ctcttgtacc
     3301 ggagaattag ctctgaaaat gaaaaaaact ctgattgagt gtaaaggctg tgaaaacatc
     3361 tgttccgtga cgaaaatgga tatgggaaac ggaaaagttt tctatgccgg aaacaactgc
     3421 gaaaaaatct ttttttccgg tggtggaaat gtgcagacac agtgtttcga tggagtttcg
     3481 tggaaaagga atcttctttt tgacagagat tcgtgtccgg aaagtgaagc tttaataact
     3541 gtggggattc cgagggcatt gaatatttat gaaaattatc ctttctggca cactcttttt
     3601 accagatgcg gcatcagaat tattctttct gatccttcga cggttcagct ttacgaatca
     3661 ggatcgggaa gtgtcatgag tgacagtatc tgtttcccgg ccaaactgat gcatggtcat
     3721 atttttaatc tcataaacag gaaagttgac aggatttttt atcccatggt tgtttatgaa
     3781 gacaaaagcg gatgctggca gaacagtttt aactgtccta tcgtttcagg ttactctgat
     3841 gttgtaaaga gttcgataaa tcctgaaaat aattacggaa taccacttga taccctggct
     3901 gtttcattta aggataataa tcttcttgaa aagaagctga aatcctattt tacaaaaact
     3961 tttggtgttt cacattaccg tttcagaaaa gcatttgccg aggcaatgaa agaaaaggat
     4021 aatttcaagg tgattctcag acaaaaatcg gatgaaataa taacaaaggc aaaacaggaa
     4081 ggaagaaaag ttgtcatgct catcggccgg ccatatcaca tcgatccgct gataaaccat
     4141 aaaattcctg aaatgattac agttttcggt tttgatgtga taacagaaga cagtgtttcg
     4201 caaacttgtg gaacggctgt aaaagttctt gatcagtggg aatattccaa caggctttac
     4261 agtgcggcat ggtatgtggc tgaaaacgat gctgtaattc ctgttcaatt gaattctttc
     4321 ggctgcggac ctgatgcaat tgcgactgat gaaatgaaag aaattctaaa gtcgtacgga
     4381 aaaaatctga ctacaatcag gattgacgaa attgtttcac ccggttcaac taaactgaga
     4441 ttgagaactt tatttgagtc aatggaaaga ggcggaaaca gaacgaaatt tcttagaaaa
     4501 gagctggctg ttctgaagga agaagatctg aaaaagcaga ttcttattcc tttttttgct
     4561 gatttttact cgccttttgt cgagcacgga ttgagagctg ccggaataaa tgtcgtttct
     4621 cttccgaaac cggatgccga atcgctgaaa gaaggtttgc ggtatgccaa taatgaaatc
     4681 tgttatccgg caataattat tgttggagac attatcaaag cattaaaaag cggaaaatat
     4741 gatcttgaaa atactgtcat aggaattaca cagacaggcg gtcaatgcag ggcgagtaac
     4801 tatttttcaa tgataagaaa agctatggca gccgccggat tcgacagagt gcctgttgtt
     4861 tctttttcta caacaaataa agccgtgaca aagcagcctg aatttaaaat cagaaaaatt
     4921 ccatttataa actggggttt gaaagcgata cttcttgctg atgctgtttc gagaatgtat
     4981 aatgccgtgg cggcaagaga aaagacaaaa ggatctgcgc tgaaactggc taattctctt
     5041 attgcggaat ggaatctgac ttcgtttgat gtcaacagaa aggagatgtt cagatttttg
     5101 aaaaaagcta ttaacagttt taacagtatc gaaatcgatc atgatccgag accgaaaata
     5161 ggtgtcgtcg gggaaattta tgtaaaatac agtgcgtttg ccaaccatga tgtgcttgaa
     5221 tggcttatga atcacggtat tgaaccagtg atgcccgatc ttgtggattt ttttgtacag
     5281 gaaataatca atgtgaaaat aaacagccgt gaaaatctga gccggaatga catgttttca
     5341 ttgtttttac cactgcttga aaagcatttt accaaatttc atgaagaagt cggaaatgaa
     5401 atgaaacggt ttaaattcag ttacccttat tacggagtga gggatcttgc cggaaaagct
     5461 tcaaaaattc tttctcttgt aaatcaattc ggtgaaggat ggctgatagc tgccgaaata
     5521 gcttcgtttg ccgaaaacgg aataaacgat gtgttgtgtc ttcagccttt cggatgtata
     5581 gccaatcagg ttgtggcgaa aggtgtcgaa aaaaagatga aggatattta tccaggtctt
     5641 gacattcttt tcatagatct ggatagtgac acatctccgg caaatatgat gaacagggtt
     5701 cactttcttg tcagaaatgc gaaaaatcat aaaatcagga ggtctcatga atgatttaag
     5761 agagaaaata atttccgaag caggaaagct tttttatact tacggattca aaagggtttc
     5821 catggatgaa atcgcttcca aatctaaaat aagcaaaaaa actctttata cggcattcgg
     5881 ttccaagaac gaaatcatga aaatatttct tgaacgcaaa gttgatcagt ttgctgatga
     5941 tgtagatgct ctttttgaca catcgctgcc tgtggaagat actttcaggg aatatatcgg
     6001 actgttcaaa agactctctt tttcgatttc aaccccgatg gtcagtgaca tgaaatatat
     6061 gcctgaaatg tttgatattg ttgataaaaa aagaagaaaa atgctgctta aatataaagt
     6121 tattctcgat aaagccaaag aagaaggaat aatcagccct gatctgaata ctgatgtttt
     6181 tatggaaata ttaatgaata caatgagttc caccattatt cctgcgaaac ttgtggaact
     6241 taacatttct ccgggagacc ttatcggaca gataataacg atcttttttt tcggtgtttt
     6301 ccggaaagaa cggggcataa ttgattaagt ctgcgaaact taattgttct gcttttttct
     6361 gtttttcttc attttggata caagaatttc cagatttttt cctaggacgg attctgcttc
     6421 tttaaataat tcttttgatg caaaatactt atctgccgca agtacgacaa gcgcttcttt
     6481 tgttgaagga aaagtttttg tatacggcca catgtgatta ataataatgt cttcttcaat
     6541 tttgttgagt gaaaaatgtt ttttagcatt tctcaatgct gttttgggat gttctttccc
     6601 gtgattatct cctttttccg gatgatctgt atgccaatca taaaggaaaa agtcatgcat
     6661 cagtgcgcct ctcgccgtcg atctgaagtc aagtttaaat ttttttgcaa gcttatatga
     6721 ccaaaatcgt tcgattctga cttaaactcc cgaaaaatgg tctgagctca gca
//
LOCUS       DAOOYB010000153         6735 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_12926_length_6735_cov_10.911228, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000153 DAOOYB010000000
VERSION     DAOOYB010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6735
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12926_length_6735_cov_10.911228"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(49..1506)
                     /locus_tag="PL056_09240"
     CDS             complement(49..1506)
                     /locus_tag="PL056_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47242.1"
                     /translation="MSEEKNVISSDFFENTERLERLRMNDFTVVFNSILKALRLYGAE
                     NETVEKVVTKFFETYKFFFISESLMTVTYNGNDFLINDVRVKKKKSSQIALDDLENFF
                     IDLQIASMTFPSDLRTKELIEFITTGHDVLKKNPGPDAVFEQLESIFHQKGVRIGLTR
                     RDSADDDDDFSILDKRQLARLSYRNMINDHSLFKSKIRNNRPIPLRKAIRNVQNVIDL
                     FSDGSSDSQESHLLMLASINSLSGKFIATHLANSTILSIAAGVQLGIDRSLLTRIGTA
                     AYFHDIAIHEKARGETVEHSQEGFAVLSRLNSLNFAMMEAAITSGLHHVTYAFDGNPL
                     PPEKPEMSTPLGEIIKVCDYYDLVTRWWPARKSVPMKRTDAIEQIFKMAQMRCFSPVA
                     AKALFSALGVFPPGTVLRVAGKNLLACSVDVFRATGQKSRAAVLDAKMKFLGIREFYP
                     QELLEIPTGLHFKMPPETIKAILDSFVPETEDNPS"
     gene            complement(1503..3545)
                     /locus_tag="PL056_09245"
     CDS             complement(1503..3545)
                     /locus_tag="PL056_09245"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HPM47243.1"
                     /translation="MVTASERKREVFEFFNSNVGNVADNTELLRSAKELVGLFGKVIR
                     GYKLYPRSNPAFSKFGDQFKTKLDDILSKIPTISLKISPKGFMVGKSVLDTNEKDKEI
                     VFFLYNDGLREIFFQKGTAREEITELFNVLAQCTLFANEDYDLATLLWDHNFPNIGYI
                     TEDELIKENVVSYEENTFSPFLVEELAYGEGIDSIGDGGEDGEGEDGDSPEDAAKGTG
                     FEHDDFEKYSTLVFKENENIDLNEIKERLDKRIENHSVGKMEMFKFDEALKKNSDSFV
                     VNRFLRELSSRLLLSQGNSSGQELLETAASLWEKLLLFGSVKGAVLFIKTLKMIAERL
                     ENDQPEYSRKIREGFSSLGDNDFLADVFSTLEDLPSDELEAIGELFQMVPSSNIGFLV
                     LKINDLESTQTRISVIESFSKFVVITEDLLVLTKHEDWKVVRNALALMKDKKDPRIVP
                     AIRNAISHPQKQARIEALALLMEFSIEEAMPALEKAVFSSEREIRSIAIRKVLELKEP
                     VVKAIVNRALQIHNLKKLEKDEVDEYFKLIIDMRREDMYDLVGNLLFSDDAEMKGKAV
                     MAIFNAPTLSPFSKFIAKASDISIMSKMKNDDLKQFCRLYKPETYKELLPKLEYLFSI
                     SGSIFNKTVTGIKETVFKALVIYIDDPSAIAFFRKGLSSGSRETVSLIEKIAAKYL"
     gene            3672..4406
                     /locus_tag="PL056_09250"
     CDS             3672..4406
                     /locus_tag="PL056_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47244.1"
                     /translation="MLSLIFSLLIAVATGLITGYLINYGFGVFIGLFAGMGMFIFFTR
                     FYSKKLQDVFTDANTYIQKQQFDKAISILRSGYKYNNHAFLVKAQIDSQIGIILYTQK
                     KFGEAYKHLQNSNPRIMMGYLMLIIGHIKNNKTKNIDRDIDLLLKFNKKDPFAYSAVA
                     YLYEEELSDREKAHSTLNKASKIMPDNQKIQEHLISFQNNKPFKMEKYGEMWYQLMLD
                     RKGITRLQNKMIKDQQKSMKVKSRIR"
     gene            4423..6255
                     /gene="typA"
                     /locus_tag="PL056_09255"
     CDS             4423..6255
                     /gene="typA"
                     /locus_tag="PL056_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018123832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HPM47245.1"
                     /translation="MHNLIRNDNIRNVAIIAHVDHGKTTLVDAMFRQSGVFREGEIVN
                     ERIMDNMDLERERGITIAAKNCSIKWKGVKINIIDTPGHADFGGEVERALSMADGAIL
                     LVDASEGPLPQTRFVLRKALEAKLQIMVIINKIDRKDARPVEVLDEIYNLFIDLGADE
                     DQLDFPILYAIGRDGIAQKKLDDKGENLHILMDMIVSEMKGPQYDPEKPFQMLVTDLS
                     YNDYMGRLAIGKVINGRVTCNKTLVCIKENGENQRLKVSKLQSYEGVKLAETTEAAPG
                     DIIILAGIEDVHIGDTVATIEEPVALKRIKVDEPTVSMIFTINNGPLAGREGKHVQLR
                     KIKERLHKETLMNVSIRLEEPPQNDHVIVKGRGELQMAIIVEQMRREGFELCVGRPTV
                     ILKEIDGKIHEPIEHVYIECHEMFTGVVTEKLSIRKGRMLNLVNSTSERAFMEFSVPS
                     RGLIGYRDQFLTDTKGTGTLNSIFDGYEEYRGNFPVRFTGSIVADRQGEAVAYALFNL
                     EPRGELFITPGMPVYEGMIIGEHSKDLDIDVNPCKAKKLTNMRASGKDDAVTLTPVRP
                     MTLEKAIHFINDDELVEITPSSLRLRKAVLSATIRHTLRKKDDK"
     gene            6257..>6735
                     /gene="bioD"
                     /locus_tag="PL056_09260"
     CDS             6257..>6735
                     /gene="bioD"
                     /locus_tag="PL056_09260"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HPM47246.1"
                     /translation="MNQFFITGIDTGIGKTVVTGLIAKYFLLIGKKATTMKPVQTGCD
                     GIAEDILVHRSIMGVEPDRFDLDGITAPFVFKYPASPHLAAELEGREIDVSSIDKSLK
                     ILAENFETILIEGAGGIYVPITRDYTTLDFIAQRSIPTIVVSSPKLGSINHTMMTLE"
BASE COUNT         2013 a         1426 c         1360 g         1936 t
ORIGIN      
        1 tgtttttaaa agactctctg tttttatctc agattttttg ggggaacttc aagaaggatt
       61 gtcctctgtt tcgggaacga acgaatcaag tatcgcttta attgtttccg gaggcatttt
      121 aaaatgaagt cctgtgggta tttctaaaag ttcctgcgga taaaactccc tgatccccag
      181 aaatttcatt tttgcatcaa gtactgccgc gcggcttttc tgtccggtag ctctaaaaac
      241 atcaacagag cacgcaagca gattttttcc cgccaccctt aatacggttc ccggaggaaa
      301 gacacctaaa gcactgaaaa gagcttttgc cgcaactgga gaaaaacatc gcatctgggc
      361 cattttaaag atctgttcta tcgcatctgt ccttttcatc ggcacgctct ttcttgccgg
      421 ccaccatctt gttacaaggt cgtaatagtc acagactttt attatttcac caagaggtgt
      481 tgacatttcg ggtttttcag gtggaagcgg gttcccgtca aatgcatatg tgacatgatg
      541 aagtcccgat gttattgctg cttccatcat ggcaaaattc agagagttga gtctgctgag
      601 aacggcaaac ccttcctgag aatgctcaac tgtttctcct cttgctttct catgtatggc
      661 tatatcgtga aaatatgcgg cagttcctat ccttgtaaga agagatctgt ctattccgag
      721 ctgaactccc gccgcaatac ttaaaattgt tgaattggca aggtgtgttg caataaattt
      781 accgctcaag ctgtttattg atgcaagcat tagcagatgt gattcctgag aatcagatga
      841 tccgtcagaa aaaagatcta tgacattctg gacatttctt atcgcttttc taagaggaat
      901 aggtctgttg tttcttatct ttgatttgaa aagagaatgg tcatttatca tattcctgta
      961 actcaacctt gcaagctgcc tcttatcaag gatgctgaaa tcatcatcat catcagcgct
     1021 gtctcttctt gttaaaccta ttctcacccc tttttgatgg aaaatgcttt caagctgttc
     1081 aaaaactgca tcgggtccgg gattcttctt tagaacatca tgtcccgtcg taatgaattc
     1141 gatcagttct tttgttctca gatctgaagg aaaggtcatg gaagctatct gaagatcgat
     1201 aaaaaagttc tcaagatcat caagtgcgat ctgggaagac ttcttttttt taaccctgac
     1261 atcgtttatc agaaaatcat tgccgttgta tgttacggtc atcagtgatt cagaaatgaa
     1321 aaagaattta tatgtttcaa agaatttggt cactactttt tcaactgttt cgttttcagc
     1381 tccgtaaaga cgcagggctt tcagaataga attgaacaca acggtaaaat cattcatcct
     1441 taatcgttca agccgctcag tattttcaaa aaaatctgaa gagatcacat ttttttcttc
     1501 gctcatagat atttcgctgc aattttttca attaatgata ccgtctccct gctgccgctt
     1561 gacaatcctt tcctgaaaaa tgcaatggcg gaaggatcgt caatgtatat gaccagtgct
     1621 ttaaaaactg tttctttaat tcctgtcact gttttgttga aaatggaccc gctgatactg
     1681 aagagatatt ccagttttgg aagaagttcc ttgtaagttt caggtttgta aagccggcaa
     1741 aattgtttca gatcgtcatt tttcattttg gacatgatcg agatgtccga tgcttttgca
     1801 atgaattttg aaaagggtga aagcgtggga gcattaaaga tcgccatgac tgccttgcct
     1861 ttcatttcgg catcatcaga aaacaaaaga ttcccgacaa ggtcatacat atcctctctt
     1921 ctcatatcaa tgatcagttt aaaatattca tccacttcat ctttttcgag tttcttcaga
     1981 ttgtggatct gcaaagctct gttgacaatg gctttaacaa cgggttcttt aagttcaaga
     2041 actttcctga tcgctatcga cctgatctcc cgttcacttg aaaacaccgc tttttccaaa
     2101 gcaggcatcg cctcttcgat ggaaaactcc attaacagcg cgagtgcttc tatccttgcc
     2161 tgtttctgag gatgggatat tgcatttctt attgcaggaa ctattcgcgg atcctttttg
     2221 tctttcataa gggcgagagc atttctcaca actttccagt cttcatgttt tgtaagaaca
     2281 agaaggtctt ctgtgataac aacaaatttt gaaaaacttt caattactga aattcttgtc
     2341 tgagttgatt caagatcatt tatcttcaaa acaaggaatc cgatattgga agacggtacc
     2401 atctgaaaaa gctctcctat tgcctcaagt tcatcggacg gcaggtcctc caatgttgaa
     2461 aagacatctg caagaaaatc gttatcgcca agtgacgaaa acccctctct tatctttctt
     2521 gaatactcgg gctgatcatt ttcgagtctt tcagcgatca ttttcaatgt ttttatgaac
     2581 agcaccgccc ctttcacaga tccgaaaaga agaagcttct cccacagcga tgccgctgtt
     2641 tcaagaagtt cctgaccgga agaattccct tgtgaaagta gaagccggga tgaaagctcc
     2701 ctgaggaacc tgttcacaac aaaactgtcg ctattctttt tgagtgcctc gtcaaatttg
     2761 aacatctcca tttttccaac agagtggttc tcaattcttt tatcaagccg ctcttttatt
     2821 tcattcagat ctatattttc gttttcctta aagaccagtg tcgaatattt ttcaaaatca
     2881 tcgtgttcaa aaccagtccc ttttgcagca tcctcaggtg aatcaccgtc ctcaccttct
     2941 ccatcctctc cgccatcacc tatgctgtca attccttcac catatgcgag ttcctcaaca
     3001 agaaaagggg aaaatgtgtt ctcttcgtac gaaacaacat tttcttttat cagttcgtct
     3061 tccgttatat atccgatgtt aggaaaattg tgatcccaaa gaagagttgc aagatcatag
     3121 tcctcattag caaaaagagt acactgggca agaacattga aaagttcggt tatctcctct
     3181 ctggcggtcc ctttctggaa aaagatctct ctcaatccat cattgtaaag aaaaaaaact
     3241 atttccttat ctttttcatt tgtatcaaga accgattttc ccaccatgaa acctttagga
     3301 gatatcttta aggaaattgt ggggatcttt gacaggatgt catccagttt tgttttaaac
     3361 tggtctccga attttgaaaa cgccggattg cttctgggat acagcttgta gccccttatg
     3421 acctttccga acagtccgac cagttcttta gcggaacgga gaagttctgt gttgtcagcg
     3481 acatttccaa cattgctgtt aaaaaattca aatacttccc gttttctttc tgacgcagta
     3541 accatagttt taaaaactcc gaaccttttc cgggtcattt tagcagattg catttaaagc
     3601 acaaagtttt tacttgaaat aagttaatcc ttaaataaat tggtgcagta tttgttttta
     3661 aggaggaatt tatgctgagc ttgatctttt cactgctgat agctgtggcg acggggctta
     3721 taaccggcta tcttatcaac tacggttttg gcgttttcat cggtcttttc gccggaatgg
     3781 gaatgttcat attctttaca aggttctatt caaaaaaact gcaggatgtt ttcaccgatg
     3841 cgaacacata tatccagaaa cagcagtttg acaaggcgat ctcgatcctc aggagcggtt
     3901 ataaatacaa caaccatgca tttctcgtaa aagcccagat cgactcccag atcggaatca
     3961 tcctgtatac ccagaaaaaa tttggtgaag cgtacaagca ccttcagaat tcaaatccga
     4021 ggataatgat gggctatctc atgcttataa tcggacacat caagaacaac aagacaaaaa
     4081 acatcgaccg tgacatagat cttcttttga aattcaacaa aaaggatcct ttcgcatatt
     4141 ctgctgtggc atatctctat gaagaagagc tcagtgacag agaaaaagct cattcaactc
     4201 tgaacaaagc ttcaaagata atgcccgaca atcagaaaat acaggaacat ctcatctctt
     4261 ttcagaacaa caaacccttt aagatggaaa aatacggaga aatgtggtat cagctcatgc
     4321 ttgacagaaa agggatcacc cgccttcaga acaagatgat caaagaccag cagaaaagca
     4381 tgaaagtaaa atccagaatc agataaatat aggaaaaatc atatgcacaa cttaatcaga
     4441 aacgataaca tcaggaatgt tgcgatcatc gcccatgtag atcacggtaa aaccacactt
     4501 gttgacgcca tgttcaggca gagcggagtg ttcagagaag gtgaaattgt aaacgaaagg
     4561 atcatggaca acatggacct tgaaagggaa agagggataa cgatcgccgc caagaactgt
     4621 tcaataaaat ggaaaggggt taaaataaac atcatcgaca ctccgggaca cgccgatttc
     4681 ggtggagaag ttgagcgtgc tctctccatg gctgatggag ctattcttct tgttgatgct
     4741 tcggaaggac ctcttccgca aaccagattt gttttaagaa aagctctcga agcaaagctt
     4801 cagataatgg tgataatcaa caaaattgac agaaaagatg caagacctgt tgaggtcctt
     4861 gacgaaatat acaatctttt catagattta ggtgctgatg aagaccagct tgattttccg
     4921 atactgtatg ctatcggaag agacggcata gcccagaaaa aacttgatga caagggggaa
     4981 aaccttcata ttctgatgga tatgatagtt tccgaaatga aagggccgca gtacgatcct
     5041 gaaaaacctt tccagatgct tgtcacagat ttgagttata acgattacat ggggcgtctt
     5101 gctatcggca aagtgattaa cggtagagtt acatgcaaca aaacacttgt ctgcattaaa
     5161 gagaacggtg agaatcaaag acttaaggtt tcaaaacttc agtcttatga aggggttaag
     5221 cttgcagaaa ctacggaggc cgcacccggc gacataataa ttcttgcagg gatcgaagat
     5281 gtccatatcg gagacacagt tgcaaccata gaagagccgg tagcgctaaa aaggataaag
     5341 gtcgatgagc cgaccgtcag catgatcttc accataaaca acggacccct tgccggtcgc
     5401 gaaggaaaac atgtccagct cagaaagatc aaggagcggc ttcacaagga gaccttgatg
     5461 aatgtctcca tccgtcttga agagccaccg cagaacgatc atgtcatcgt aaaagggaga
     5521 ggcgaacttc agatggcgat cattgtagaa cagatgcggc gtgaaggatt tgagctttgt
     5581 gtaggaagac cgactgtaat tctcaaagag atcgacggta aaatccacga accgatcgaa
     5641 catgtctata tcgagtgcca cgaaatgttc acaggggttg taacagaaaa actttcgatc
     5701 agaaaagggc ggatgctcaa ccttgtcaat tcaacttccg aaagggcttt catggaattt
     5761 tcggttccgt caagagggct tatcggttat cgtgatcagt tccttaccga cacaaaagga
     5821 acgggcactc ttaattcaat ttttgatggc tatgaagagt atcgcggcaa ttttccggtg
     5881 agattcaccg gttcgatagt tgcagaccgt caaggcgaag ccgttgcata tgcgcttttc
     5941 aaccttgaac cgagaggcga acttttcata actcccggaa tgccggtgta tgaagggatg
     6001 ataataggtg aacattcaaa agacctcgat attgatgtca atccatgcaa agcaaaaaaa
     6061 cttacaaaca tgagagcatc gggcaaagac gatgcggtaa cgcttacacc ggtaagaccg
     6121 atgacccttg aaaaagcgat ccattttata aacgatgatg aactcgtcga gatcacccct
     6181 tcatcattac ggcttcgaaa agctgttctt tctgcaacga taaggcacac attaagaaag
     6241 aaagacgaca agtgacatga atcagttttt tatcacagga attgacaccg gcataggaaa
     6301 aacggtcgta accggtctga tcgcaaagta ttttctctta atcggtaaaa aagcaacgac
     6361 catgaagccg gttcagaccg gatgtgacgg cattgctgaa gatattcttg tacatagatc
     6421 aataatgggt gttgagccag accgtttcga tcttgatggg atcactgccc cttttgtttt
     6481 caaatatcct gcaagtccac atcttgccgc tgaacttgag ggtcgcgaaa tagatgtcag
     6541 ctcaatagat aaatccctga aaatacttgc tgaaaacttt gaaacaatac ttatcgaagg
     6601 ggctggcgga atttatgttc cgatcacaag agattataca acgcttgatt ttattgctca
     6661 aagatccatt ccgacgatag tggtttcttc accaaagctc ggaagcataa accacacaat
     6721 gatgacactc gaaat
//
LOCUS       DAOOYB010000154         6615 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_13253_length_6615_cov_11.107470, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000154 DAOOYB010000000
VERSION     DAOOYB010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6615
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13253_length_6615_cov_11.107470"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..1511
                     /locus_tag="PL056_09265"
     CDS             117..1511
                     /locus_tag="PL056_09265"
                     /inference="COORDINATES: protein motif:HMM:NF014335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HPM47247.1"
                     /translation="MNIRFFIFSMFLLSFFIINAAENEPVTEKKEERGFIFGSYGRVQ
                     PATDLEGGSAKWVNIVGHGSRLEQGSYVELDFAYLFEQPAKGPKFDFVSTLAFSESLF
                     HSNGKWAALNTLRNLFVRAENIIWKPLNMWVGSRMYRGDDIYLLDFWPLDDANMYGGG
                     IGLKFDTWTVDYYIGFNRLEIDWQYQEKQVVTDTFGAESITWMDRQRLVTGLKYEHKY
                     QLSDKGTGIKWKLFAELQRIGEGEKYNDPVDDENLISYPEDLGWSVGGQLGFYGYQKN
                     SFFNMFAKHSGGLAAYGLLSVPWGFDNEYKTTNAKEFLFGLSGNTEFKHFGNMCGAYV
                     RYFEDADPNKYDNDDFVEFIFAIRPHVVLHEYFYLAFELSHQLKKTNGLTRLSDDSEK
                     NILPQVTKISFIPIVTIGGGNYARPQLRLVYTAAFQNDDTKALYNKDDIRSQRSVLHY
                     FGLSAEWWFNVGHR"
     gene            1521..3488
                     /locus_tag="PL056_09270"
     CDS             1521..3488
                     /locus_tag="PL056_09270"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPM47248.1"
                     /translation="MNKFRYILFAIISVSAFSCVDVDKIEGPVDITNHVEDWRDEVIY
                     QLITDRFADGDVNNNHKVNKLSLTAWNGGDFQGIIDNIDYLKELGVTTLWISPVVKNV
                     DEDAGIAGYHGYWTQDFKGVNPHFGDLAKLREMVQILHENDIKVILDIVANHIGQLFY
                     YDMNKNDQPDEMVMGSNMTCSQYCSHIVNVENEQAGIEICKQEYNAGFTMGGDNSECF
                     NSCVDRCENEDYPQKMGLKRYSEWDPDFDPRKIRAFSEAGESGLAPLKWVYMPEINRV
                     PPNPPEFHNDEWYNRMGRTVSWDSDYQVVKGDFPGGLKDLDTTKTDVRNALISVFQYW
                     ISAINIDGFRIDTIKHVEHEFWQVFTPAMRDYAESLGKENFFIFGEAFDGNDELLGSF
                     TKNNEMDSVFYFSQKFQVFDSIFKYGGPTKNFERLFNLRMNKLDDGSPLPEGEKPRYG
                     TVSGVTDADGKVLSPQKIMVNFMDNHDLPRFLYEQPDETALRNALTLMLTMDGIPCIY
                     YGTEQNFKGGNDPANREPLWDSGYDTENATFRHISAINSLRAGYAPLRRGNMSIIWST
                     DNTNDEPDAGILAFERTYKKEKVLVVINTSKTKSSTTSNEETVMKTGFAAGAKLKNVF
                     PGSDGKTYTVDSDQTLELTLPKQSSMILVKN"
     gene            3579..4670
                     /gene="ugpC"
                     /locus_tag="PL056_09275"
     CDS             3579..4670
                     /gene="ugpC"
                     /locus_tag="PL056_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014750579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HPM47249.1"
                     /translation="MASVSFEKVVKKYSEEAVILKGIDLEVGDGEFLVLVGPSGCGKS
                     TLLRTLAGLETITSGNIRIGERIVNNVAPKDRDIAMVFQSYALYPHMTVRENMSFALK
                     VRNFEKKEIEKRVNAAAELLDITHLLDRLPKQMSGGQRQRVAMGRAIVRDPKVFLFDE
                     PLSNLDASLRTQMRVELKKLHERLKTTIIYVTHDQVEAMTLADRIVVLNKGILQQVGT
                     PAELFDTPANVFVAGFIGSPSMNLLNLKTIKRGDHATLVSDGINIGIVFSEGCFREEM
                     VVGIRPQDFMLTDGDPEISGTVEIVEALGRQSLVHVKLSDTAKIAAELETVLARNFKI
                     GDKIKLNIKHEKMHFFDSVTKERINGEIK"
     gene            4667..>6615
                     /locus_tag="PL056_09280"
     CDS             4667..>6615
                     /locus_tag="PL056_09280"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPM47250.1"
                     /translation="MRKFTLILFFLIFHLFIYAQKTDVTIWHSYRGSEQTALEELKNK
                     FNELDNSFNVVLLSVPYDAFANKLTNAIPRGNGPDAFIFAHERIGDWAESSIIRPLET
                     QTVENLRTDTIPSTLEALKYKGKYWGYPLSFKSVVLFYRTDLVKKVPASTSEMIELAK
                     KLTDPAKGNYGLAFEATSVYFSAPFIYGFDGGFCFEEGKTKKTGDACLNNEGNIAALD
                     FIGNLVNGLKIVPQEATSALVTQLFNDGKAAMVINGPWFLGEIRKDVPFDVAVLPTVS
                     QTGNVLKPFLTVESYLIAEHPTVNVSGARAFGNFITSFDGAKVRAVTGRQSVSTKSVY
                     SDPDIVSDNILNTFRVQAEIAVPMSNDPKMRMVWEPFAGALRKVLRGAENPKTALDAA
                     QAQYVIFSRPLPKAKSPVIFVVILLIAGLTGLLILVRQIRKHKFFQNVMNSPTPYLYL
                     FPAMFSMLLLVFVPFTVGTAVAFFAHRSGEYQFVGFSNFVSILLSKDFPITNPLSFYF
                     TLAVTVMWTAVNVFLHVSIGLILALMLREPWLKMKGFYRMLLIIPWAVPNYITALIWK
                     GMFNRQFGAINGILQSLGMEPVSWFSSFWTSFAANVTTNTWLGFPFMMVTALGALQAI
                     PGELEDAASVDGANGWERFRHVILPL"
BASE COUNT         1956 a         1324 c         1531 g         1804 t
ORIGIN      
        1 aaaaagaaca tcagaaatct attcatttcc cttgaaaaag tgaaatgaca tcacaaattt
       61 gacttaaaaa ccaccctccg ttaaaattat ccgctctaac ttttgaggtg gtgcagatga
      121 atatcagatt ttttattttc tcaatgttcc tgctgtcgtt ttttattatc aatgccgctg
      181 aaaatgaacc tgttacagaa aaaaaagagg aacggggttt tattttcggt tcttacggca
      241 gggttcagcc cgcaaccgac cttgagggag gaagtgccaa atgggtcaac attgtcggtc
      301 acggaagccg tcttgaacag ggaagttatg tcgaacttga ctttgcgtat ctttttgaac
      361 aaccggcaaa agggccgaag ttcgattttg tttcaacact cgctttttca gaatcacttt
      421 ttcacagcaa cggaaaatgg gcggcactga acactttgag aaacctgttc gtccgtgcag
      481 agaacattat ctggaaaccg cttaacatgt gggtcggaag cagaatgtac cgcggggatg
      541 acatatatct tcttgatttc tggccgcttg atgatgcaaa tatgtacggc ggcggcatcg
      601 gacttaagtt tgacacttgg acagttgatt actatatcgg tttcaaccgt cttgagatcg
      661 actggcagta tcaggaaaaa caggtggtta ctgacacttt cggggctgaa agcattacat
      721 ggatggaccg tcagcggctt gtaacagggc tgaaatatga acacaaatat cagctcagtg
      781 acaaaggaac agggataaaa tggaagcttt ttgctgagtt gcaaagaatc ggtgaaggtg
      841 agaaatataa cgatcctgtc gatgatgaga atcttatctc ctatcctgaa gatctcggct
      901 ggtcagtcgg tggtcagctt ggattttacg gttatcagaa aaacagtttt ttcaatatgt
      961 ttgctaaaca ttccggaggt cttgccgcct acggactgct gagcgttccg tggggttttg
     1021 acaatgaata caaaacaacg aatgccaaag aatttctgtt cggtctttca ggtaatacgg
     1081 aatttaagca tttcggcaac atgtgcgggg cttatgtcag atattttgaa gatgcagacc
     1141 cgaacaaata cgacaatgat gactttgttg agttcatttt tgcgataagg ccccatgtgg
     1201 ttctccatga atatttctat cttgcatttg aactgagcca ccagcttaag aagaccaacg
     1261 gactgacgcg gcttagcgac gattccgaaa agaacattct gccgcaagtg acaaaaattt
     1321 catttattcc gatagttacg ataggcggtg gaaactatgc aagacctcag ctgcggctcg
     1381 tctatactgc cgcattccag aatgacgaca caaaagcgct ttacaataaa gatgatatca
     1441 ggtcacagcg gtcagtgctc cactatttcg gactgagtgc cgagtggtgg ttcaatgtcg
     1501 gtcacaggta ggaggaaaaa atgaataaat tcagatacat actgtttgcg atcatttcag
     1561 tatcagcttt ttcatgtgtc gatgtagata agatcgaagg gccggttgac attacaaatc
     1621 atgttgagga ctggcgggat gaagtgatat atcagcttat caccgacagg tttgcagatg
     1681 gtgatgtgaa caacaaccat aaagtgaaca aattgtccct cacggcctgg aacggcggcg
     1741 attttcaggg aatcattgat aacattgact atctgaaaga acttggtgtg acaactcttt
     1801 ggataagtcc tgttgtaaaa aatgttgatg aagatgcagg cattgccggc tatcacggtt
     1861 actggactca ggatttcaaa ggggtcaacc cgcacttcgg cgatcttgca aaactgcgcg
     1921 aaatggttca gatccttcat gaaaacgaca taaaagtcat ccttgacatt gtcgcaaatc
     1981 atatcggtca gctcttttat tatgacatga acaagaacga tcagcccgat gaaatggtga
     2041 tgggaagcaa catgacctgt tcacagtact gctctcatat agtcaatgtc gaaaatgagc
     2101 aggcgggaat tgagatatgt aagcaggaat ataatgccgg tttcacaatg ggtggagaca
     2161 attccgaatg tttcaacagc tgtgtggaca gatgcgaaaa tgaagattat ccgcagaaaa
     2221 tgggactgaa aagatattca gagtgggacc ccgattttga tccaagaaag atacgggcat
     2281 tttcagaagc gggtgaatca ggtcttgctc cgcttaaatg ggtctatatg cctgaaatta
     2341 accgagttcc tccgaatccg cctgaatttc acaacgatga gtggtacaac agaatgggaa
     2401 gaactgtttc ctgggacagt gattatcagg tcgttaaagg tgatttccct ggtggtttga
     2461 aagatcttga cacaacgaaa actgatgtca gaaacgctct gatctctgtc tttcagtact
     2521 ggataagtgc aataaacatt gacggtttca ggatcgacac catcaagcat gtggagcatg
     2581 agttctggca ggtttttact cctgcgatga gagattatgc agaaagtctc ggcaaggaga
     2641 atttctttat tttcggagag gcgtttgatg gaaatgatga acttttagga agtttcacaa
     2701 aaaacaacga aatggattct gttttttatt tttctcagaa atttcaggtt tttgactcaa
     2761 tatttaaata tggcggaccg acaaagaact ttgaaagact tttcaacctc agaatgaata
     2821 aacttgatga cggctcccct cttcctgaag gtgaaaaacc aagatatgga actgtttcag
     2881 gtgtgactga tgctgacggg aaagtattaa gtccgcagaa gataatggtc aatttcatgg
     2941 ataaccacga tctgccgagg ttcctgtatg aacagcccga tgaaactgct ttgagaaatg
     3001 ctcttacact tatgctgaca atggatggaa ttccatgtat ttattacgga actgaacaga
     3061 atttcaaagg gggtaacgac cctgcaaacc gtgaaccgct ctgggacagc ggatatgata
     3121 cagaaaatgc aacattcaga catatttcag ctattaattc tctaagagcc gggtatgcac
     3181 cgttaagaag aggcaacatg tcgatcatct ggtcaaccga taacacaaac gatgaacctg
     3241 atgccggaat tcttgcgttc gaaaggactt acaaaaaaga gaaagtgctc gtagtaataa
     3301 acaccagcaa aacaaagtca tcaactacat caaatgaaga gaccgtcatg aaaaccggct
     3361 ttgccgcagg ggcaaaactg aaaaatgtct tccccggatc agatggaaaa acatatacag
     3421 ttgacagcga tcagaccctt gaactcactc tcccgaaaca gtcctcaatg atacttgtga
     3481 aaaactgatc tcccgaaaga aatctaaaaa acaccttctt tttgaattat tttttttatt
     3541 actgtataat cacttaattt cagttccgga ggtttgacat ggcatctgtg agttttgaga
     3601 aagttgttaa gaaatacagc gaggaagctg taatccttaa agggatcgac cttgaagtgg
     3661 gtgatggtga atttcttgtt cttgtcggcc cttccggttg cggaaaatcg actctgctta
     3721 gaactcttgc aggtcttgaa acgataactt cgggaaatat caggatcggg gaacgaattg
     3781 tgaacaatgt cgctcctaaa gatcgggata tcgcaatggt tttccagagt tatgcccttt
     3841 atcctcacat gactgtcaga gagaacatgt cgtttgcctt aaaagtaaga aattttgaaa
     3901 agaaggagat tgaaaaaagg gtcaatgcgg cggcggaact tcttgatata acgcatcttc
     3961 ttgaccgcct gcctaaacag atgagtggtg ggcagagaca aagggtggca atgggaagag
     4021 cgatagtgag agatccgaaa gtattccttt ttgatgaacc cctttcaaat cttgatgcat
     4081 cgcttagaac tcagatgaga gtcgagctca aaaaacttca tgaacggctt aaaacaacga
     4141 taatttatgt cactcacgat caggtcgagg cgatgacgct tgccgacagg atcgtcgttc
     4201 tgaataaagg gatattgcag caggttggaa ctcctgcgga actttttgat actccggcaa
     4261 atgtttttgt cgccggtttc atcggcagtc cgtcaatgaa ccttctaaac ctcaaaacca
     4321 taaaaagggg cgaccatgcg acactcgttt cagacggtat aaacatcggc atagtctttt
     4381 ctgaagggtg tttccgtgaa gaaatggttg ttgggataag accgcaggat tttatgctca
     4441 cagacggtga tcccgaaatt tcaggaacag ttgagatagt tgaagcgctc ggaagacagt
     4501 cgcttgtcca tgtgaaactt tctgacactg ccaagatcgc cgcggaactc gaaacggttc
     4561 ttgcaagaaa tttcaagatc ggcgacaaaa taaaactgaa tatcaaacat gaaaagatgc
     4621 acttttttga ttcagttaca aaagaaagga tcaacggaga aataaagtga gaaaattcac
     4681 tcttattcta ttttttctga tctttcatct tttcatctat gcacagaaga ccgatgtgac
     4741 catctggcat tcatatcggg ggagcgaaca gactgctctt gaagaattaa agaacaaatt
     4801 caacgagttg gacaacagtt tcaatgttgt gcttctgagt gttccatacg atgcctttgc
     4861 aaataagctc actaacgcca tcccgagagg taacggtcct gacgctttca tttttgccca
     4921 tgaaaggatt ggcgactggg ctgaaagttc gataataaga ccgcttgaaa ctcaaactgt
     4981 tgaaaattta agaactgaca ctattccttc aactctcgaa gcattgaaat ataaaggaaa
     5041 atactggggc tatccccttt catttaaaag cgtcgtcctt ttttacagaa cagatcttgt
     5101 gaaaaaagtt cctgccagca ctagtgaaat gatcgagctc gcaaaaaaac taactgatcc
     5161 ggcaaaggga aattatggtc tcgcttttga agcaacttca gtatattttt cagccccgtt
     5221 catctacggg tttgacggcg gattctgctt tgaagaggga aaaactaaga aaacaggtga
     5281 tgcatgcctt aacaacgaag ggaacatcgc ggcacttgat ttcatcggaa accttgtgaa
     5341 cggattaaaa attgtcccgc aggaagcgac aagtgctctg gtgacccagc ttttcaatga
     5401 cggtaaggcg gcaatggtga taaacggacc gtggttttta ggcgaaataa gaaaagatgt
     5461 cccttttgat gtagcagttc ttccgacagt ttctcaaacc ggcaatgtgc tcaagccttt
     5521 cttaaccgtt gaatcctacc ttatcgctga gcatccaaca gtaaatgtga gcggagcaag
     5581 agcattcgga aatttcatca cttcatttga tggtgcaaag gtccgtgcag tgacgggaag
     5641 gcagtcggtt tcaactaaaa gcgtttacag cgatcctgat atagtaagcg ataacatttt
     5701 gaatacattc agggttcagg cggaaattgc agtcccgatg agtaacgatc cgaagatgag
     5761 aatggtctgg gaaccatttg cgggtgcttt aagaaaagtg cttcgcggtg cagaaaatcc
     5821 caaaaccgct cttgatgccg cacaagctca atatgtgata ttttcaagac ctcttcccaa
     5881 agcaaaaagt cctgtgatat ttgttgtgat actgctcatt gcaggtctga ccggactgct
     5941 cattcttgtg cggcagatca gaaaacataa atttttccaa aatgtgatga acagtccaac
     6001 accgtattta tatctttttc cggcgatgtt ttccatgctt cttcttgttt ttgtcccttt
     6061 tactgtcgga actgccgttg cattttttgc tcaccgcagc ggagaatatc agtttgtcgg
     6121 gttttcaaat tttgtgtcaa ttcttctttc aaaggatttt ccgataacca accccctctc
     6181 tttttatttc acccttgcag taacagtgat gtggactgct gtgaatgtgt ttctccatgt
     6241 ttctatcggg ttaatacttg cgttgatgct tcgtgaaccg tggcttaaaa tgaaaggttt
     6301 ttaccgtatg cttctaataa ttccgtgggc agttccgaat tatatcacag ctctcatctg
     6361 gaaagggatg ttcaacaggc agttcggtgc aataaacggc attctccagt cactcggaat
     6421 ggagccggtg agctggttca gctcgttctg gacatcgttt gccgcgaatg tgacaacaaa
     6481 cacctggctt ggattcccgt tcatgatggt gacggcactc ggagcgcttc aggcgatccc
     6541 cggagaactt gaagatgccg caagcgttga cggagcaaac ggttgggaaa ggttcagaca
     6601 tgtaattctg ccact
//
LOCUS       DAOOYB010000155         6608 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_13271_length_6608_cov_6.570731, whole genome shotgun sequence.
ACCESSION   DAOOYB010000155 DAOOYB010000000
VERSION     DAOOYB010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6608
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13271_length_6608_cov_6.570731"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..548)
                     /locus_tag="PL056_09285"
     CDS             complement(36..548)
                     /locus_tag="PL056_09285"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HPM47251.1"
                     /translation="MKIILIPLIIILMLSVGSCGEINTGDSGNTGNTGDTGDNFPNEH
                     DGLNWSDASSDSMPWDEAITYCENLGGRLPTISELRTLIQNCPGSETGGECKVADDCL
                     SFEGCYSDQCNGCEKTNSGKYSVFGDTYWFSSSSETSEYTDFAWHVSFNGGQVGNSGK
                     NSSFYVRCVQ"
     gene            complement(589..870)
                     /locus_tag="PL056_09290"
     CDS             complement(589..870)
                     /locus_tag="PL056_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47252.1"
                     /translation="MSLKDKLKKPVKTPNDIINEAPADKEIEKTLVNPKRRKVDAAEE
                     KQTERVAVYFTKNEIKELKKFCFDNDVKMAAFLREKALSTLKRSNAKAI"
     gene            complement(854..1495)
                     /locus_tag="PL056_09295"
     CDS             complement(854..1495)
                     /locus_tag="PL056_09295"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPM47253.1"
                     /translation="MIITVGNQKGGCGKTTLAVNLACLYAQKGTTLLIDADTQQSSML
                     FREARPDTAAHFSAVANHTATIHEDVKTFTHNTIIIDAGGRDNKPFRSSVIASDLLII
                     PVTPSPVDLWSAEDTFRLYQEIKMIKNIKGLIVLNMVQPGVNITGDVIDILKEYSEKY
                     QLKVLDSMISFRTAFKESFAEGLSVAEMKGEKFKKAAEETINFFNEVNHVFKG"
     gene            complement(1897..3444)
                     /locus_tag="PL056_09300"
     CDS             complement(1897..3444)
                     /locus_tag="PL056_09300"
                     /inference="COORDINATES: protein motif:HMM:NF021637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HPM47254.1"
                     /translation="MSGKKKNLPQTESTSYSYKAPTPKVEEEIYNLQEDSSLIQYEMN
                     MIELPFFSRDKNVGEAVIKKYIFSGNQYMEVIPSSDKSSGYKIPQEFDEKIFYAIMQL
                     YRKQGKRKIITTIYELLKLAGYGDRKEEYERAKQSLDRLSGTEYVFSGIMYDKKNNRR
                     IKERVKNTILQRYGIREIDDLSDEEKEAFGREKVNKNVVFLEISSFIEENIEHKGFLY
                     YDAEKLLDVTNSTARKLYLLISKWQGWEKSDTIRRSCRFLASRIPLSWEKSNIPGTVN
                     VLEKAAEELKERQMIGSYYLERKKPLSTSEIIFNFKGANETAKTKAIMNANLDLSTET
                     GHEGIVIDAVRDEFLDDRQATIFDMTTTKGTEDLLNLLPEEYRTETNRKTIERFYAKG
                     SEHIKSNIRYTLKNCNGNFEAYLNKALAGDWAASEREKTEIERDKEKKEQIEAEKKTE
                     DLRKKATEIYENLKEDDLNKMHREAEHSTYYRFSFEDRVNKGDIKKEDALKEVSLVLI
                     SGRIQKN"
     gene            4894..5367
                     /locus_tag="PL056_09305"
     CDS             4894..5367
                     /locus_tag="PL056_09305"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HPM47255.1"
                     /translation="MDRVILDGRPHFIWIFLKYIAVFIVVVFSVFSYFYFRHPVIKAA
                     SPVILILSATAGLILTIYYFSYGYKITGERIAFRSGIFNVKTKFIELYRVKDIEMIEP
                     FFYRFFGLANIKITSSDHDMPHFVLFAQKKQLEPVIRKSVEEIRKIKGVREIDTV"
     gene            5386..5619
                     /locus_tag="PL056_09310"
     CDS             5386..5619
                     /locus_tag="PL056_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47256.1"
                     /translation="MNYDEAIEIVRSSSREDWICESDTNSFTYKRNLCLHIKQLEIDR
                     HDLFNEDWATKHPDKKAYKVAFFADSPKNDIET"
     gene            6128..>6608
                     /locus_tag="PL056_09315"
     CDS             6128..>6608
                     /locus_tag="PL056_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47257.1"
                     /translation="MRKVFFVLVFLFTVFFVYGEYPDFKYSRIVLADVYVPSIGEFVV
                     SYGLNENNQQILYILDEVDLGPGEETGVCWSEGSGNDMWPSRFGIIILRTKCELGSAY
                     TDPQGCPPYNSTCTPTSIHNFARIKKLQGMCENTVVEGCLSKTGLTWDAPTGWSVEEL
                     "
BASE COUNT         1843 a         1323 c         1166 g         2276 t
ORIGIN      
        1 ttaaacgcta aagcaacaag ctgttgtgtg gttgactact ggacacagcg gacatagaag
       61 ctgctgttct tgccgctgtt gcccacctgt ccgccgttga aactcacatg ccatgcaaag
      121 tctgtgtact ctgatgtctc cgacgacgac gagaaccaat aggtatctcc aaaaactgaa
      181 tatttgccgg agttggtctt ttcacaccca ttacattgat cactatagca accctcaaaa
      241 gataaacaat catcagcaac tttgcattcg ccaccggttt cagatcccgg acaattttga
      301 ataagtgttc taagttctga gattgtcggg agccgcccac caagattttc acaataagtt
      361 attgcctcat cccaaggcat agagtcactt gaagcatctg accagttcaa gccgtcatgt
      421 tcatttggaa aattatcacc ggtgtcgcct gtgtttcctg tattgccaga gtcaccagta
      481 ttaatctcgc cgcacgaacc caccgaaagc atcaaaataa taatcaatgg aatcaggatg
      541 attttcatca taaccccctt cttttttaag caataaagca acaaagcgtt aaattgcttt
      601 agcgtttgaa cgcttaagcg ttgaaagtgc cttttccctt aagaatgcag ccatcttaac
      661 atcattatca aaacagaatt tctttaattc tttgatctca ttctttgtaa aatagacagc
      721 aactctttca gtctgttttt cttctgctgc atccactttt cttcttttgg gatttacaag
      781 agtcttttca atttctttat ccgcaggagc ttcattgatg atgtcattcg gtgtttttac
      841 cggtttttta agtttatcct ttaaagacat ggttcacctc attaaaaaag tttattgttt
      901 cctcggccgc ttttttaaat ttttcccctt tcatttcagc aacggaaaga ccttctgcaa
      961 aactttcctt gaaagcggtc ctgaaagaga tcattgaatc aaggacttta agttgatact
     1021 tctcagaata ttctttgaga atatcgatca catcaccggt gatgttcact cccggttgta
     1081 ccatattgag gacgattagc ccctttatgt tctttatcat cttgatttcc tggtaaagac
     1141 ggaaagtatc ttccgcactc cacagatcca caggggacgg agtcaccgga ataatgagaa
     1201 ggtcggaagc gatcacggaa gatctgaaag gtttattgtc ccttccccct gcatcaatga
     1261 taattgtgtt gtgtgtaaaa gtcttaacat cttcgtggat ggttgccgta tgattggcaa
     1321 cggctgaaaa atgggcggca gtgtccggac gggcttcacg gaaaagcata cttgactgct
     1381 gagtgtcagc atcaattaaa agggtagtcc ccttttgagc ataaagacac gcaagattga
     1441 cagcaagcgt ggttttacca caaccgccct tctgatttcc tacagtgata atcatctgtt
     1501 acctctttta aaaagcgtta aagcaacaaa acgccaaagc attatagcgt ttaatgtaca
     1561 aagctttaaa gcgttagtca agaaaacttg attgataccc ggataatctc tataattata
     1621 gttaagttgg ggctactctt tcgagcagtc tttttttttg cattttgatg tgtcgggggg
     1681 attttcaggt tactgaaaaa caaaaacccc cggcactcaa agtgtcgagg gtcttataaa
     1741 aatccaatgc acaccagtta attattttat cccgactgtt tataatgtca aaaatagtcc
     1801 tgctgaatgc aggacagtga tcggattgga attgaaactt gtggaatgaa taccgcatga
     1861 agcggcacag ttaagataac agttaaccgg aataattcaa tttttctgga ttcttcctga
     1921 aattagaaca agggacacct ctttcaatgc atcttctttt ttaatatccc ccttgttcac
     1981 cctgtcttca aatgaaaatc tgtaatatgt agaatgttcg gcctctctgt gcattttatt
     2041 aagatcatct tccttcagat tttcatatat ctctgtagct tttttccgta gatcttcggt
     2101 tttcttttct gcctctatct gttctttttt ctctttgtct ctttcaatct cagttttttc
     2161 tctttcgctt gccgcccagt ctccggcaag ggctttgtta agatacgcct caaaattacc
     2221 gttacagttt ttaagagtat atctgatgtt cgactttata tgttcagatc cctttgcata
     2281 aaaccgttca attgtttttc tgtttgtttc agtgcggtat tcttccggga gaagatttaa
     2341 gagatcttct gttccttttg ttgtggtcat atcaaagatg gttgcctgtc tgtcatcaag
     2401 aaattcgtcc cgtacagcat caataacaat tccttcatga cccgtttcag tgctcagatc
     2461 taagtttgcg ttcattattg ctttggtctt tgctgtttcg ttggctccct taaaattgaa
     2521 tattatctct gatgttgaga gcggtttttt tctttcaaga taataactgc cgatcatctg
     2581 tctttcctta agttcttccg ccgctttttc caacacattt actgtgccgg gaatatttga
     2641 tttttcccat gaaagcggaa ttctggaagc aagaaatctg caagatcttc ttatggtgtc
     2701 gcttttttcc catccctgcc attttgaaat gagaagataa agttttcttg ctgttgagtt
     2761 tgtcacatca agaagctttt cggcatcgta ataaaggaat cctttgtgtt cgatattttc
     2821 ttctataaag cttgagatct ccagaaaaac aacgttttta ttaacttttt ctctgccgaa
     2881 tgcttctttt tcttcatcac taagatcatc aatttctctt attccgtacc tttggagtat
     2941 tgtgtttttt actctctctt ttattctccg gttattcttc ttgtcataca ttattcccga
     3001 aaacacatac tcggtcccgc taagccggtc gagactctgt tttgcacgct catattcttc
     3061 ttttctgtcg ccatatcccg caagtttcag cagctcgtat attgttgtta taatctttct
     3121 cttgccctgt ttcctgtaga gctgcattat tgcataaaag atcttttcat caaactcctg
     3181 cggtattttg tacccggagg acttgtctga tgaaggaatt acttccatat attgatttcc
     3241 tgagaaaata tatttcttaa taacagcttc acccacattt ttatctctac tgaaaaaagg
     3301 cagttcgatc atgttcattt catactgtat aagggagctg tcttcctgta gattataaat
     3361 ctcctcttcc accttaggtg tcggagcctt atatgaataa gatgtcgatt cggtttgagg
     3421 cagattcttt tttttcccgg acatcatacc ctccgatcta ccgcagtttc atcataaaat
     3481 agctgcaaca tactactatc attaatcttt ttgcactgac tctttctgtt ctcaatcatc
     3541 gattcacctg tcaatctagt ttaatcacgg cctttttacc gcagtttcat cataaaatca
     3601 agctttttac cgcagtttca tcatagtttt tttattagaa cggtctaaat taccgcagtt
     3661 tcatcataaa actgtccctt gatgctccca atacaggctt tttaccgcag tttcatcata
     3721 gaaactaccg cagtttcatc atagaattta ccgcagtttc atcatagaat ttaccgcagt
     3781 ttcatcataa acccccctta tctatccccc ctttaatgta gtaactatat aaaaacaaca
     3841 acaacaatgc gcgcgcgagg agtcgagcga agcgagtcga aaaatttcaa atttcccccc
     3901 gaacccccca aataaggata tttttgcgca cctttccgct tcgcaagctc gcttcaagga
     3961 tttacgccaa tgcaaaaata tctctgtcgc ttaaacccgt tacaaattca aaaattgtgg
     4021 ttacgctgtc tttcaaacta agaggatatt ttgcgggcac ccctcgatca ttcgacaagc
     4081 tcatgactcg gggattacgc cttgtcgcaa aatataccgt tcagatattc aggtataatg
     4141 cttacattcc ttaagtcact aattacattc tacgcccttt aaaacttatt ataaaaacaa
     4201 atgtcattca tattcaattt gtaccactaa cgacctgaga agtgctttct tttataccct
     4261 taaagaccgt atttcacttt taacgcattt tcagggttta tccgaggtct tttcctagat
     4321 tcagttttct ccgagcttat acagcgggag attgacccgc cctgaaaatg cggccggatt
     4381 aaacatttgg tacaaatccg tcacggaaga tctgacatag aactcattaa acaaacaggg
     4441 agaattcccc acggcaatct taacaactct ataatttaac tgaatatatt cacttttaca
     4501 gcttccagaa ttttgaattt ttttcataat ttatttctga tgaaccggtg gaagcgaagc
     4561 tgtcaccagc cgccacatga attttcaagc tattgttttt gttatgaata ttctttattt
     4621 gcgggttttt taaacattta gggagaatcc gtcacggaaa tttccataaa tgcttgatct
     4681 aattgagaaa acttgttttt acaccttcca aaagtgaatg tttttttttg atttttgatt
     4741 tagcgtgaat taattaacca ataaattcaa ttagttgaaa gctactttgc acttcagaac
     4801 agcttcgctt ccaccatata taatttcatt agattttttg attttcattt ttgattgaag
     4861 taaaatgtag catgattttg aagaggtgtt ctaatggaca gggtcattct tgacggcaga
     4921 ccgcatttta tatggatatt tctgaaatat attgctgttt ttattgttgt tgttttttct
     4981 gttttttctt atttttattt cagacatccg gttattaaag cggcctctcc tgttatactt
     5041 attctttcgg ccacagctgg actgatcctg acgatatatt atttttcata cggttataaa
     5101 atcaccggtg agcggatcgc tttcaggtcg ggaatattca atgttaaaac aaaatttatt
     5161 gaactgtaca gggttaaaga cattgaaatg attgaaccct ttttttacag attttttggt
     5221 cttgcgaaca ttaaaataac aagctcggat cacgatatgc cgcattttgt gttatttgca
     5281 cagaaaaaac agcttgaacc ggtcataaga aaatcagttg aagagatccg gaagataaaa
     5341 ggtgtgaggg agatagatac ggtttgatta tcaggagaaa ttaaaatgaa ttatgatgaa
     5401 gctattgaaa ttgtaagatc ttcctcccgt gaagattgga tttgtgaaag cgatacaaac
     5461 tctttcacct acaagcgtaa tctttgttta cacattaagc aattagagat tgaccggcac
     5521 gaccttttta atgaagactg ggctacaaag caccctgata aaaaagcata taaagtggcc
     5581 ttttttgcag attccccgaa aaacgacata gagacctaaa actgactatt taacacaatt
     5641 ccacttttgc gaatattggc acataatgac ataatattat tggactatta cttttaattc
     5701 caaacataca ttaacacatt gagcttcatg ttaactgttg ttcggcataa aatatatgtt
     5761 ctgcaaattt tctacgatat tcttccagag cattgacagc ataaagtctt tctgtaattt
     5821 ggacaagcaa gtccggttat tgtcaagctc ttttataata agcttttaac tgaatggctg
     5881 tcggaaagtc ttttactgaa gaggtattaa tacattgcta tattaaaaac ccttggtttt
     5941 aaagggcatc tgtgaatgta caaaaattaa tgcagtttta acaaaaattt atgttgaaaa
     6001 aatcagattt ttttaatatg attctttata tggtgaacta cccctctgat gctgataaga
     6061 cagaaattcc ttctgcttca ggacttttaa gataaaagtt ttaatagttt tttgtcggag
     6121 gtgtgttatg agaaaggtgt tttttgtttt agtatttcta tttactgttt tttttgtcta
     6181 tggtgaatat cctgatttta aatattcaag gattgtgctt gctgatgtct atgtgccatc
     6241 tataggggaa ttcgttgtta gttatggttt aaatgaaaat aatcagcaaa ttctttatat
     6301 tcttgatgaa gttgatcttg gtccaggaga agaaactgga gtctgttgga gtgaaggttc
     6361 tgggaatgat atgtggccat cgcgatttgg aattattata ttacgcacaa aatgtgaact
     6421 tggatcggct tacactgatc cgcaaggatg tcctccatat aattctacat gcactcctac
     6481 ttccatacat aattttgcaa gaattaagaa attacaagga atgtgtgaaa ataccgtggt
     6541 ggaaggatgt ctatcaaaaa cagggttgac atgggatgct ccaactggtt ggagtgttga
     6601 agagttat
//
LOCUS       DAOOYB010000156         6592 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_13307_length_6592_cov_15.240630, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000156 DAOOYB010000000
VERSION     DAOOYB010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6592
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13307_length_6592_cov_15.240630"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..584
                     /gene="rsgA"
                     /locus_tag="PL056_09320"
     CDS             <1..584
                     /gene="rsgA"
                     /locus_tag="PL056_09320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HPM47258.1"
                     /translation="VSPGVPVHAVSARENHIETLKPYLIAGKTVALLGSSGVGKSTIV
                     NVLLGKKIQSVNEISDSVHKGRHTTTTRQLFLLDNGAMLIDTPGMREIQFWGEEESIS
                     AVFEDIEDIMSKCRFSDCTHNGEPGCAIAKALETGELDQGRYQNYLSLQAELALLEQR
                     KTKSKQQMKKEWEKEIAKKVKEFNKSEKGKFGR"
     gene            774..2078
                     /locus_tag="PL056_09325"
     CDS             774..2078
                     /locus_tag="PL056_09325"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM47259.1"
                     /translation="MKRFLLDELLKWKESSIRKSLILRGARQVGKSYLIREFGKHFDS
                     FVEVNFEKDHNLSQIFDGDLEPQKIIEKISAAKNTAVIPGKTLLFLDEIQAVPRAVTA
                     LRYFYELMPQLHVISAGSLLEFVLEKTGMPVGRVSSMYLYPLSFKEFLAASGREMLCD
                     YIDKHDSQNPVSEVFLDQLMTLFGEYMAVGGMPEAVSVWLEHRDLKKVSEIHHNIIDT
                     YRQDFLKYAKNQQIHHVDKVFSAVPRMLGRKFVYSRVDETLKSREIKPALELLEMAQV
                     VHRVSHSSSNGVPLGAECNYSIFKTIFIDVGLAQAILGLSDGEWILDVKNSVVNRGEI
                     VEAFVGTEILAYSDPHRPQKLFYWVREKQNSQAEIDFVIQKQDKVIPVEVKSGTTGNL
                     KSMMQFLKEKPSTPYGIHISPNNFGIHNNINQIPLCAIWKMF"
     gene            2089..2565
                     /locus_tag="PL056_09330"
     CDS             2089..2565
                     /locus_tag="PL056_09330"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013751430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HPM47260.1"
                     /translation="MFNIVLINPEIPQNTGNIARSVLALKCRLHLVKPLGFSIDDTML
                     KRAGLDYWQFVDVILWESVQNYFENNDIDRMHFFSKKGKKRYDLTDFQPGDHLVFGCE
                     SNGLSDEILQKHQDNTVFLPIMDTKVRSINLSSAVSVALFEAMRKNGAFDQLSRFE"
     gene            complement(2544..3023)
                     /locus_tag="PL056_09335"
     CDS             complement(2544..3023)
                     /locus_tag="PL056_09335"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPM47261.1"
                     /translation="MKLRDKIVQTDLEFIREILVSTGFFYDFEVATAVEIGEEVLAKG
                     DAAGYHFLFVEDEDFKPYGFTSFGDIPCTKNRFDLYWIGVHEKMRGKGIGSVLLKESE
                     KAVKSLDGKILYIETSARELYNPTRGFYLKNGYKIETILEDFYDDGDGKVIYSKRLN"
     gene            complement(3007..4002)
                     /locus_tag="PL056_09340"
     CDS             complement(3007..4002)
                     /locus_tag="PL056_09340"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HPM47262.1"
                     /translation="MEKNSSHEILIFHQFVPDDALPDEKDVLEQAEEISKVLVRSGYS
                     VRAVSVDDKFLFIPDILKKPYAVFNLVETFNGSAVNAHLVPLMLEQFGIPFSGNSSSA
                     IFLTTNKLLTKDILFANNIPTANWHIKGRDEEFQKDLYIFKPVSEDASIGITDENLKI
                     LESSQQADEAIEYFEKKYNLPYFCEKFIDGREFNVSVIGSKGEPKVLFPAEMVFFQEG
                     HENKILCYDSKWNEESEKYDSSRRSFDLGDEDRKLIKKLKSICEKCWKIFGLSGYVRV
                     DFRVDGSNNPYVLEINANPCLSPDSGIFAAAKNEGMNYDQLINKILEECFNEIKR"
     gene            complement(3980..4975)
                     /locus_tag="PL056_09345"
     CDS             complement(3980..4975)
                     /locus_tag="PL056_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HPM47263.1"
                     /translation="MKIGFTYDLKDDYIKMGFSTVEVAEFDTVETIEGIESSLRKLGY
                     SVDRIGSVKSLISRLNAGDKWDLVFNIAEGVSGIGREAAVPAILDVYNIPYVFSDPMV
                     LSLTLHKGMTKRIVRDAGIKTPQFQIVYCSGDIKNVDIPYPLFAKPLSEGTGKGIGAL
                     SMINNGGDLEKACLQMLKEYNQPVLVEKFLPGREFTVGITGTGSDAKVVGMMEINFLA
                     EAESAAYGLVNKKNYEKLMKYTLPEKEMYDKCAETALAAWRVLECRDGGRIDLRNNES
                     EEPEFIEVNPLAGLNPIDSDLPILSRMNGLDYDGLIKQIMDSAIKRIFDGKKQQS"
     gene            complement(5000..6232)
                     /locus_tag="PL056_09350"
     CDS             complement(5000..6232)
                     /locus_tag="PL056_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HPM47264.1"
                     /translation="MKKEMRAVEEEVPLVVGLSALRPLSSFSSKQCSTASLFQRKFFP
                     ESSQSDWTSWKWQIQNSITDSKKLSEMFALTKDELDALNSGEVTLPFRITPYYASLLY
                     GRESDYALRKTMIPLSDEFFTSFGESGDPLCEEHMSPVPNVVHRYPDRVLFLATEFCS
                     SYCRYCTRHRVVGNKNELHRFSSKSWENGINYIASHPEIRDVLISGGDPLTMPDARID
                     YLLSRLRSIPHVEIIRIGTKVPAVLPQRITPKLVRMLKKYHPLFISIHFTHPDEMTLE
                     TSVACRALADAGIPLGSQTVLLKGVNDDSKVLTDLYHRLLMVRVRPYYLYQCDPVQGS
                     SHFRTSVETGLRIIEQIRGFTSGYAVPTYVIDAPGGGGKIPILPDYYQGRDDNYVYLK
                     NFEGKKYRYPDPVRTPSL"
BASE COUNT         1935 a         1357 c         1318 g         1982 t
ORIGIN      
        1 ccgtttctcc cggtgtgccg gttcacgcag tttcagcaag ggaaaatcac attgaaacac
       61 tgaaaccgta tctgatcgcc gggaaaactg ttgctctgct cggttcttca ggggtaggca
      121 aatcaacaat cgtgaatgtg ctccttggaa aaaagatcca gagcgtaaac gaaataagcg
      181 attctgttca taaaggccgt cacacaacaa cgacaagaca gctttttctc ttggataacg
      241 gagcgatgct catcgatact cccggaatgc gtgaaattca gttctgggga gaagaggaaa
      301 gtatttctgc ggtttttgaa gatattgaag acatcatgtc aaaatgcaga ttttcagact
      361 gcactcacaa tggagaaccg ggttgcgcaa tagccaaagc acttgaaaca ggagagcttg
      421 atcagggaag atatcagaat tatctttcac ttcaagcgga actggcactg cttgagcagc
      481 ggaaaacaaa atcaaaacag cagatgaaaa aagaatggga aaaagaaatc gctaaaaaag
      541 tgaaagaatt caataaaagc gaaaaaggaa aattcggcag ataaattcca gcggtcagaa
      601 taaatcaatt tttgctgtga aatacggttt ttcgggacaa catccccatg tttttgtgca
      661 gatatgttca aaacacccca tctgttttgt gcaaatttgt tcaaaacagg tattgtgttt
      721 tgtgcaaata ataatataat gggctttgtt ctcaggtttt aaggagggga aaaatgaagc
      781 ggtttcttct tgatgaactt ttaaaatgga aagagagttc aatccgtaag tcattgattt
      841 tacggggagc aaggcaggtc ggaaaaagtt atctgattag agagttcgga aaacattttg
      901 acagttttgt cgaggtgaac tttgaaaagg accataatct ttctcaaatt tttgacggag
      961 atctggagcc gcagaaaata attgaaaaaa tatcggctgc caaaaataca gctgtcattc
     1021 cgggaaaaac acttcttttt cttgatgaaa tacaagcagt tccaagagct gtcaccgctt
     1081 tgagatattt ttatgaactg atgcctcagc ttcatgtgat ctctgccgga tcacttcttg
     1141 agttcgtgct tgaaaaaacc ggaatgcctg tcggaagagt gagcagcatg tatctttacc
     1201 ctctttcgtt caaagagttt cttgctgcat caggcagaga aatgctttgt gattatatcg
     1261 ataaacacga ttctcaaaac ccggtttcag aggtttttct tgatcagttg atgactcttt
     1321 tcggtgaata tatggctgtg ggaggaatgc ccgaagcagt gagtgtctgg cttgaacata
     1381 gagatctgaa aaaagtcagc gaaattcacc ataatattat tgacacatac agacaggatt
     1441 ttcttaaata tgcaaaaaat cagcagatac accatgttga caaagttttt tcagccgtgc
     1501 cgagaatgct tggcaggaaa tttgtttatt caagggttga tgaaactctg aaaagccgtg
     1561 aaataaaacc ggcacttgaa cttcttgaaa tggcacaggt ggttcacagg gtcagccact
     1621 cctcttctaa cggcgttccg cttggggctg aatgtaatta ttcaattttt aagacgattt
     1681 tcattgatgt cggtcttgct caggcaattc ttggactcag tgatggagag tggatactgg
     1741 atgttaaaaa cagtgttgta aacagaggtg agatcgttga agcttttgtc ggaactgaaa
     1801 ttcttgcata cagcgacccg catagaccgc aaaaactttt ttactgggta agggaaaagc
     1861 agaattctca ggcagaaatt gatttcgtga tccagaaaca ggataaagtg attccagttg
     1921 aagtaaaaag cggaacaaca gggaatttaa aaagcatgat gcaattcctg aaagaaaaac
     1981 cgtcaactcc ttacggaatc cacatttcac caaacaactt tggaattcat aacaatatca
     2041 atcagatacc gctttgtgcg atctggaaaa tgttttaacg gagcattcat gttcaacatt
     2101 gttctgataa atccggagat tccgcagaat accggaaaca tcgctagaag cgtccttgct
     2161 ttaaagtgcc ggcttcatct tgttaagccg ctcggttttt caattgatga tacaatgcta
     2221 aaaagggcag ggcttgacta ctggcagttt gttgatgtta ttttgtggga aagtgtgcag
     2281 aattattttg aaaataacga tattgacaga atgcactttt tctccaaaaa agggaaaaaa
     2341 agatatgacc tgaccgattt tcaaccggga gatcaccttg ttttcggttg cgaaagcaac
     2401 gggcttagcg atgaaatact tcagaaacat caagacaata ccgtgttttt acccatcatg
     2461 gacacaaaag tgagaagcat caacctttca agcgcagtca gcgtcgccct ttttgaagca
     2521 atgagaaaaa acggtgcttt tgatcaatta agccgctttg aatagatcac ttttccgtca
     2581 ccgtcgtcgt agaaatcttc aaggatggtt tcgattttat atccgttttt aagatagaat
     2641 cctcttgtgg ggttatagag ttcccttgcg gatgtttcga tataaagaat ctttccgtcc
     2701 agtgatttta ccgctttttc cgattctttg agaagaactg agccgatccc ttttcctctc
     2761 attttttcgt gaactcctat ccagtaaagg tcaaaacggt tctttgtgca tggaatgtcg
     2821 ccaaatgaag taaatccata cggcttgaaa tcctcatctt caacaaaaag gaagtgatag
     2881 cccgccgcgt caccttttgc aagcacctct tctcctattt caactgcggt agcaacttcg
     2941 aaatcataga aaaaccctgt tgaaaccagg atttcccgga taaattcaag atccgtctga
     3001 acaattttat ctcttaattt cattgaagca ctcctcaaga attttattta taagctgatc
     3061 atagttcatt ccttcatttt tcgccgccgc aaatattccg ctgtcgggac ttaagcaagg
     3121 atttgcgttg atctcaagaa catatggatt gttcgatccg tcaactctga aatctactct
     3181 cacatagccg gaaagaccga atatcttcca gcatttttca caaattgatt tcagtttttt
     3241 tatcaatttt ctgtcctcgt ccccgagatc gaaggatctt ctggatgaat catatttttc
     3301 agattcttcg ttccacttcg agtcataaca gagtatcttg ttctcgtgtc cttcctgaaa
     3361 aaacaccatt tcagcaggga aaagaacttt cggctctcct ttgctgccta tcactgaaac
     3421 attgaactct cttccgtcaa tgaacttttc acagaaatat ggcagattat atttcttttc
     3481 aaaatattct attgcttcat ccgcctgctg cgaactttca agtattttca gattttcatc
     3541 tgttatccct atcgaagcat cttcagaaac cggcttgaaa atataaagat ccttctgaaa
     3601 ttcttcatct cttcctttta tgtgccagtt tgcagtggga atattgttgg caaaaagaat
     3661 gtcttttgtc agaagtttgt tggtcgtcag aaatatcgcc gaagatgaat tgcccgaaaa
     3721 agggatcccg aattgctcaa gcataagcgg gacaagatgg gcgttgaccg cactcccgtt
     3781 aaatgtttca acaagattga aaactgcata cggctttttt aatatatcag gaataaaaag
     3841 gaatttgtcg tctacagaaa cagctcttac gctgtagccc gatctgacaa gcacttttga
     3901 gatctcttcc gcctgctcaa gaacatcttt ttcgtccgga agggcatcat cagggacaaa
     3961 ttgatgaaag ataagaattt catgactgct gtttttttcc atcgaaaatc ctctttattg
     4021 ctgaatccat gatctgtttt atcagtccgt cataatcaag accgttcatt cttgaaagaa
     4081 ttggaaggtc cgaatcaatt ggattaagcc ccgcaagagg attgacttcg atgaattccg
     4141 gttcttcgga ctcattgttt ctcaggtcaa ttcttccccc gtcacgacac tcaagaactc
     4201 tccatgcggc aagtgcagtt tcagcacatt tatcgtacat ctccttttca ggaagagtgt
     4261 atttcatcag tttttcataa tttttcttat taacaagacc gtatgcggca ctttccgctt
     4321 cggcaaggaa atttatctcc atcattccga caacctttgc gtcgctgcct gttccggtta
     4381 ttcctacagt aaattcgcgg cctggaagaa atttttcaac aagcaccggc tgattgtatt
     4441 ctttaagcat ctgcagacac gctttttcaa gatcaccgcc gttgtttatc attgacaaag
     4501 cgccgatccc cttgcctgtc ccttcagaaa gcggctttgc aaaaagagga taagggatgt
     4561 caacattttt tatatcaccg gaacagtaaa ctatttgaaa ttgaggggtt tttatgcctg
     4621 cgtctctcac tatccgcttt gtcatcccct tatgaagagt aagagaaagc accatcggat
     4681 ccgaaaacac ataaggaata ttgtaaacat cgagtatcgc aggaacagca gcctcccttc
     4741 ctataccgct gacaccttca gcaatattga atacaaggtc ccatttgtct cctgcattca
     4801 atctcgaaat aagagatttt acagatccga tcctgtcaac agaataaccg agtttcctta
     4861 gtgaactctc aataccctca atcgtttcga cagtgtcaaa ttcagcaact tcaactgtag
     4921 agaaacccat tttaatatag tcgtctttaa gatcgtatgt gaaaccgatt ttcatttttc
     4981 acctttaaaa gatggtaaac taaagcgatg gagttcttac cggatcggga taccggtatt
     5041 tttttccctc aaaattcttc aggtaaacat agttgtcgtc ccttccctga tagtagtcag
     5101 gcagtatcgg gatctttcca ccgccgccgg gcgcatcaat aacataggtc ggaacggcat
     5161 atccgctggt aaatcccctt atctgctcaa ttatcctgag tccggtctca acgcttgtcc
     5221 tgaaatgtga agagccctga acagggtcac actgataaag atagtaaggt cttacacgga
     5281 ccatcagcag ccggtgataa agatctgtca gcactttcga atcatcattc acacctttaa
     5341 gcagaacagt ctgacttcca aggggaattc cggcatctgc aagggctctg catgcaacag
     5401 atgtttcaag agtcatctca tccggatggg taaaatgaat gctgatgaac agggggtgat
     5461 attttttcag cattcttaca agtttcggtg tgatcctctg cggaagcaca gccggaactt
     5521 ttgtccctat cctgatgatc tcgacatgag gaatggaacg caatcttgaa agaagataat
     5581 ctatccttgc atcaggcatt gtaagaggat ctcctcctga aatgaggaca tctcttattt
     5641 cgggatgtga tgcaatgtaa ttgatcccgt tctcccatga tttagagctg aacctgtgaa
     5701 gttcgttctt gtttccgacg accctgtgtc tcgtacagta tctgcaatag gaactgcaga
     5761 attctgtggc aagaaaaaga actctgtcgg gataacggtg aacaacattg ggaactggag
     5821 acatgtgctc ttcgcacaaa ggatctccgc tttccccgaa actggtaaaa aattcatctg
     5881 aaagaggtat cattgttttt ctgagagcat aatcactttc cctgccgtaa agcagagaag
     5941 catagtaagg ggtgattctg aaaggaaggg tcacttcgcc actgttcaat gcatccagtt
     6001 catctttagt caaagcaaac atttcggaaa gtttttttga atctgtaatg ctgttctgga
     6061 tctgccattt ccaacttgtc cagtcactct gacttgattc gggaaaaaat tttcgttgaa
     6121 acagtgaagc agttgagcac tgctttgagg aaaaggaact taatggtctt agcgcactaa
     6181 gaccgacgac aagtggcact tcttcttcaa cagctctcat ctcttttttc ataatacccc
     6241 cttcttttaa aaaaaactaa aaaaaatgta gccggttgct cctgacaata ttttacactt
     6301 taatattaaa atgcaaatat tttttttata tatgtttttt ttagtaattt caaaatgttg
     6361 agtgactaaa aatccttttt tcagccacat ttttttaatg ttcaaaatta tatgtattaa
     6421 aaaatgacac atttaaaaac actttttttt taaattcgat actttttcga agttattttg
     6481 ttttttctgc aaactctcct tctctgctca gaatttgctt gacagaatgt tactcaagtg
     6541 tagaatcgct ccttgtggat ttgcggttta tgccgaattt tatatttttt tt
//
LOCUS       DAOOYB010000157         6566 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_13393_length_6566_cov_11.739057, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000157 DAOOYB010000000
VERSION     DAOOYB010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6566
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13393_length_6566_cov_11.739057"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="PL056_09355"
     CDS             complement(<1..289)
                     /locus_tag="PL056_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47265.1"
                     /translation="MPNSQKLSKLKKYYEITLETITPVHVGSGEEYINGLDFIGDIFF
                     DINRLMREASTDSKMMNNLCNEIEKGNLRNFYNSNRELFSKISEKKSIPNSI"
     gene            complement(289..1266)
                     /gene="csm4"
                     /locus_tag="PL056_09360"
     CDS             complement(289..1266)
                     /gene="csm4"
                     /locus_tag="PL056_09360"
                     /inference="COORDINATES: protein motif:HMM:TIGR01903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated RAMP protein Csm4"
                     /protein_id="HPM47266.1"
                     /translation="MLAYKLKFISPLHLGTDSDDQTTIEPILHSDTLFNSLIINWSLL
                     FEEAIPDLIVNPPCKLSSAFPFVAEQLFFPVPVGMFDEIMKDESVDIKKWKKVDYIPL
                     EILKKYLKNEKSELIKNDFEEFSKKHSCYSEKNRLEFGKSSERPRLTLDRVTNSSVEG
                     AFFYSRDFFFNSDAGLFILVEIENQNEKKFEAALTLLGENGIGADRSTGKGQFVWEKC
                     SVDLEKAGEKDKALLMSLYYPEMSEIDAGFLEDSSYSLIKRSGHIGHYLAGNIKRNEI
                     RMLSVGSILSAKGRRGGKIVKVVDKEKMSDAPFDVFRDGRGFLIKFGGE"
     gene            complement(1276..2034)
                     /gene="csm3"
                     /locus_tag="PL056_09365"
     CDS             complement(1276..2034)
                     /gene="csm3"
                     /locus_tag="PL056_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010421342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated RAMP protein Csm3"
                     /protein_id="HPM47267.1"
                     /translation="MTESTLKLEKKVKINGKIRLMTGMHIGGTDIGLAIGGADKIVVR
                     NTIDKVPYIPGSSLKGKMRSLIEKIEGKVNFSIKDGKYKGEICTDVAEPLVQLFGIAS
                     DNPHKNAPTRLMVRDAKMNNESVKTLENLDQTDMYLTEIKSENYIDRLTSAANPRSFE
                     RVPAGVDFDFEMIVDIYNVDKENDMQKKLLDIVKQGMKLVELDYIGGHGSRGYGQIKF
                     HDIKIQVKTAENYKDGKDWDENHQLEVKFDDFKI"
     gene            complement(2060..2563)
                     /gene="csm2"
                     /locus_tag="PL056_09370"
     CDS             complement(2060..2563)
                     /gene="csm2"
                     /locus_tag="PL056_09370"
                     /inference="COORDINATES: protein motif:HMM:NF015695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated protein Csm2"
                     /protein_id="HPM47268.1"
                     /translation="MEKYTLDYDQKCISKEEVKKWVENGPESNLIEKTEGTGKYLSSI
                     KQYETERNGTIKTDKNGRQKTKDQELTTSQIRAIFTKLKEIDAKPGNFEDTKKAELLM
                     MKPLVAYAAGRHNKKGIEVFKDYIINPGIDKIFEATQPDEMNKRFKNFVKLLEAVLAY
                     HKAHGGN"
     gene            complement(2563..5070)
                     /gene="cas10"
                     /locus_tag="PL056_09375"
     CDS             complement(2563..5070)
                     /gene="cas10"
                     /locus_tag="PL056_09375"
                     /inference="COORDINATES: protein motif:HMM:TIGR02578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated protein Cas10/Csm1"
                     /protein_id="HPM47269.1"
                     /translation="MNRDYEILTLAALLHDVGKFVERAGETKYGEPPTSFDELYSHAW
                     FSRKFLSEFFGWCEEKDTNGTDLTDLASFHHNPESVYDWILAEADRQASGHDRADGGE
                     EELLERKHYNQYGKERVPLYPITERASLILFKDNKKFTVEKTEKVNSFYKLKRMSSGE
                     LFSTKKEAYLAEDFKNSYPAHFQDFKKSLTNFVFSAQKEKSILKFKKSVETLLKEYFW
                     CIPQDTRLHAMPDVSLYDHMRLTAMLAGALFHYHNSTNTMTIEDVRNRTDQKYLLFAG
                     DISGIQNFIYDVDSKGAYKALKGRSFFMQILPVIIAKRILDKLEMDETQIIYANGGHF
                     YMILPNIQKVKEIIIEETEHINYELLKEFDGSVFLRSGHKAFNPELLQNKADNTILDK
                     NNFCALWDDVQRTLAESDRHKYSKISRQHFSEIFGPAEHISEKDRKKNENERNKFFEE
                     IGRTLKTANYLSIHKSDSKSHKILGYSFNMIEKIDDYGDLYVLDPKKLDEVFKSEKPR
                     NITLFPTGGLKGFGENTFDEIAAEAKGSHLLGILRMDVDNLGKIFAKGLRNYRHELNY
                     AREDFQVMGNMSKFNFYSIGRVTTLSSQLSMFFSSILSNIIDKNENFAEKAIIVYSGG
                     DDLFILGQWHIIPEIAITIKKEFERFVCNNPAFSLSGGIALVPGKYPIYKAAELAGEA
                     EEQAKSYKRNEKEKNAISFMGRAFDWDEIEDLQNKVAEIAEIPNSKPIINRINLIASS
                     YETTKDEIKRIMAKPIDKEKVLQMAEAEKWRWQMVYSLSRLMKQHKEYDESAVKKIEN
                     IQKYFTEKVLKIGRTGIENAEILGIWLSNITRKMEKK"
     gene            complement(5086..6135)
                     /locus_tag="PL056_09380"
     CDS             complement(5086..6135)
                     /locus_tag="PL056_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HPM47270.1"
                     /translation="MKQQIEKREDMLFENISRIINEAKSQVIKTVNTTMVSTYFEIGR
                     IIVEHEQGGKDRAEYGKETLKELSLKLSKEFGNGFSLSNLKNMKQFFITYREKTKSQS
                     LISQSSNSLDLFDLSWTHYLMLIRIDDELKRSFYEIETKKESWSVRELKRQMDSALFE
                     RLALSKDKDKVFELAQKGQLLTKAEDVVKDPYILEFLGLEEKSEYSETEFEQAIIDNL
                     QKFLLELGKGFTFVARQKRISFDERHFYIDLVFYNRLLKSFVIIDLKKGDLKHQDIGQ
                     MQMYVNYYDREVKTEDENRTIGIILCMDKSDMLVKFTLPEENDQIFASKYKLYLPTKE
                     ELVQYIENIKEKFEN"
     gene            complement(6146..>6566)
                     /gene="cas6"
                     /locus_tag="PL056_09385"
     CDS             complement(6146..>6566)
                     /gene="cas6"
                     /locus_tag="PL056_09385"
                     /inference="COORDINATES: protein motif:HMM:NF021551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CRISPR system precrRNA processing
                     endoribonuclease RAMP protein Cas6"
                     /protein_id="HPM47271.1"
                     /translation="TNSGKGAKKKYIIEFISPLRFKKNGQYVDKISYADILKGLRRRV
                     EILSGMYGESSPVPEIKEIKDIDEKLVSSDIRWVENKRWSGRQKTTMLLGGIKGTMEL
                     SGSFTPFERSLLEAGELFHFGKNAGFGLGRIKVRELR"
BASE COUNT         1843 a         1299 c          947 g         2477 t
ORIGIN      
        1 aaattgagtt aggaattgat tttttctcag aaatttttga aaaaagttct ctgtttgagt
       61 tataaaagtt tctcaaattt cctttctcaa tttcgttgca taggttattc atcatttttg
      121 aatctgtcga agcttcacgc atcagacgat taatatcaaa aaatatatct ccaataaaat
      181 caagtccatt tatgtattct tcgccacttc caacatggac tggagtaatt gtttcaagcg
      241 tgatttcata atattttttt agttttgaaa gtttctgaga gttcggcatt attcacctcc
      301 aaattttatg agaaatcctc tgccatctct gaaaacatca aaaggagcat cactcatttt
      361 ttctttgtca actactttga ctattttccc acctcttcgt ccttttgcag aaagaattga
      421 tcctactgac aacatcctta tttcatttcg tttgatgttt cctgctaaat aatgtccgat
      481 atgaccgctt cttttaatca gtgaataact tgaatcttca agaaaaccag catcaatttc
      541 agacatttct ggataataaa gcgacatcag caaagcctta tctttttctc ccgctttttc
      601 aaggtcaaca ctgcattttt cccagacaaa ctgtcctttt cctgttgagc ggtcagcacc
      661 aataccgttt tcaccaagca gtgtcaatgc tgcttcaaac tttttctcat tttgattttc
      721 aatttctaca agaataaaaa gtccagcatc actattaaaa aagaaatctc tgctgtagaa
      781 aaaagcacct tcaacactgg agtttgttac tctgtcaagt gtaagtcttg gtcgctcgga
      841 acttttgcca aattcaagtc tgtttttctc agagtagcaa ctatgtttct tggaaaactc
      901 ttcaaaatca tttttaatta gctctgattt ttcattttta agatattttt tcaatatttc
      961 aagaggaata tagtcaactt ttttccattt cttaatatca accgactcat ctttcataat
     1021 ttcatcaaac ataccaacag gaacagggaa aaagagttgt tctgcaacaa acggaaaagc
     1081 agagcttagc ttgcagggag gatttactat taaatcaggt atcgcctctt caaaaagcaa
     1141 agaccagtta ataatcagtg aattaaagag cgtatctgaa tgaagaatcg gctcaattgt
     1201 tgtctgatca tcgctgtctg tgccaagatg aagcggcgaa ataaacttta atttatatgc
     1261 cagcataaca actccttaaa ttttaaaatc gtcaaatttt acttcaagct gatgattttc
     1321 atcccaatct ttgccatctt tgtaattttc agctgttttt acctgaattt ttatgtcgtg
     1381 aaattttatc tgaccatatc ctctgctgcc gtgaccgcca atataatcaa gctcgacaag
     1441 tttcattcct tgcttaacaa tatcaagcag ttttttctgc atatcatttt ccttatccac
     1501 attataaata tcaacaatca tctcaaaatc aaaatcaaca ccagcaggaa ctctttcaaa
     1561 acttcttgga tttgcagcag atgtcaatct atcaatatag ttttctgatt taatttcagt
     1621 gagatacatg tctgtctggt caagattttc aagtgttttt acactttcat tattcatttt
     1681 tgcatcgcga accataagtc ttgttggagc gtttttgtgg ggattatctg aagcaattcc
     1741 aaacaactgt acaagaggtt ctgctacatc agtgcatatt tcccctttat actttccatc
     1801 ttttatagaa aaatttactt tcccttcaat tttttcaata agacttctca tttttccttt
     1861 gagtgatgat ccgggaatgt agggaacttt gtcaatagta tttcttacaa caattttatc
     1921 tgcgccacca attgcaagac caatgtctgt tccgccaata tgcattccag tcataagtct
     1981 gatttttcca ttgattttta cttttttctc aagtttaaga gtgctttctg tcatgatttt
     2041 ctccattatt tttaattaat taatttccgc catgtgcttt gtggtaagcc aaaactgctt
     2101 caagcagttt tacaaagttt ttgaatcttt tgttcatttc atctggttgt gttgcttcaa
     2161 atatcttatc tattccagga tttataatat aatctttgaa aacttcaatt ccttttttat
     2221 tatgccttcc tgccgcataa gcaacaagag gtttcatcat tagaagttca gctttttttg
     2281 tatcttcaaa atttccaggt tttgcatcaa tttctttaag ttttgtaaaa attgctctaa
     2341 tttgagatgt ggttaattcc tgatcttttg ttttttgcct tccattttta tcagtcttaa
     2401 ttgttccatt tctttcagtt tcatactgtt ttattgatga aagatacttg cccgttcctt
     2461 ctgtcttttc aataagatta gattccggtc cattctcaac ccattttttc acttcttctt
     2521 ttgaaataca tttttgatca taatctaatg tgtacttttc catcattttt tctccatctt
     2581 tcttgttatg ttactaagcc atatacctaa tatttctgcg ttttctattc cagtcctacc
     2641 gattttaaga actttttctg taaaatattt ttgaatattt tcaatttttt ttaccgcact
     2701 ttcatcatat tctttgtgct gtttcataag tcttgaaagg ctgtaaacca tttgccaacg
     2761 ccatttttca gcttcagcca tttgaagaac tttctcttta tcaattggtt ttgccatgat
     2821 tcttttgatt tcatcttttg ttgtttcata gcttgaagca ataagattta tgcggttaat
     2881 tatcggctta ctgtttggaa tttcagcaat ttcagcaact ttgttttgaa gatcctcaat
     2941 ttcgtcccaa tcgaaagctc ttcccataaa tgagattgca tttttctctt tttcatttct
     3001 tttgtagctt tttgcctgct cctcagcctc tcccgcaagt tctgccgctt tataaatcgg
     3061 atatttcccg ggaacaagag caattccgcc tgaaagtgaa aaagcaggat tgttgcacac
     3121 aaatctttca aattcttttt taattgtaat agcaatttca ggaattatat gccactgtcc
     3181 aaggataaaa agatcatcgc caccgctgta aacaataatt gctttttcgg caaaattttc
     3241 atttttatca ataatatttg agagaataga actgaaaaac attgagagct gagaagacag
     3301 agttgtcact ctgccgattg aataaaaatt gaatttactc atattaccca taacttgaaa
     3361 atcttctcta gcatagttaa gttcatgtcg gtaatttcta agaccttttg caaatatctt
     3421 tcccagatta tcaacatcca ttcttaagat gccaagaaga tgacttcctt ttgcttcagc
     3481 agcaatttca tcaaaagtat tttcaccaaa acctttcaat ccgccagtcg ggaataatgt
     3541 aatatttctt ggtttttcgc tcttaaatac ttcatccagt tttttaggat caagcacata
     3601 caaatcccca taatcatcaa tcttttcaat catattaaag ctgtatccaa gaattttatg
     3661 agattttgaa tcacttttat gaatcgaaag ataatttgca gttttcagcg ttcttccaat
     3721 ctcttcaaaa aacttgtttc tttcattctc attttttttt ctatcttttt cagaaatatg
     3781 ttccgccgga ccaaaaatct ctgaaaaatg ttgtcttgaa atttttgaat atttatgtct
     3841 gtcactttct gcaagtgttc tctgtacatc atcccataaa gcacaaaagt tgtttttatc
     3901 aagaatagtg ttgtcggctt tgttttgcaa caattctgga ttaaacgcct tgtgcccact
     3961 tcttaaaaaa acagagccat caaattcttt taaaagttcg tagtttatgt gttcggtttc
     4021 ttctattatg atttccttaa ctttttgaat atttggcaga atcatataaa aatgaccgcc
     4081 atttgcatat ataatctgtg tttcatccat ctctaatttg tcgagaatcc ttttcgcaat
     4141 aataactgga agaatttgca taaaaaatga ccgaccttta agagctttgt aagccccttt
     4201 actgtcaaca tcataaatga aattctgaat gcccgaaatg tctccggcaa aaagcagata
     4261 tttttgatct gttctgtttc tcacatcttc aatagtcatt gtatttgttg aattatgata
     4321 atgaaaaaga gctccggcaa gcattgccgt cagcctcatg tggtcataca gagaaacatc
     4381 aggcatcgca tgaagtcttg tgtcctgagg aatgcaccag aaatattctt ttagaagtgt
     4441 ttcaacagat tttttaaatt tcagaataga tttttccttt tgtgcagaaa aaacaaagtt
     4501 tgttaaagat tttttgaagt cctgaaaatg tgcaggatag gaatttttaa aatcctcagc
     4561 aagatacgcc tcttttttag ttgaaaaaag ttcccctgaa gacattcgtt taagtttgta
     4621 aaaactgttt accttttctg ttttttcaac agtaaatttc ttattatctt tgaaaagaat
     4681 aagacttgct ctttctgtaa ttggataaag cggaactcgc tcttttccat attgattgta
     4741 atgtttcctt tctaagagct cttcttctcc tccatcagct ctgtcatgac cggaagcctg
     4801 tctgtctgct tcagcaagaa tccaatcata aacactttcc gggttgtggt gaaaacttgc
     4861 caaatcagtc aagtcagttc catttgtgtc tttttcttcg caccagccga aaaactctga
     4921 taaaaatttt cttgagaacc atgcgtgtga ataaagttcg tcaaagctgg tgggtggctc
     4981 tccatatttt gtttcaccag ctctttcaac aaacttcccc acatcatgca gcaatgcagc
     5041 aagtgtcaaa atctcataat ctctgttcaa atcaacctcc ctatctcaat tctcaaattt
     5101 ttccttaata ttttcgatgt attgaaccag ctcctctttt gtcggcagat atagcttata
     5161 cttgcttgca aatatctgat cgttttcttc cggaagtgtg aattttacca gcatatctga
     5221 tttatccatg cagagtatta tgccgattgt tctgttttca tcttcagttt ttacttctct
     5281 gtcgtaataa ttgacataca tctgcatctg tccgatatcc tgatgtttca gatcgccttt
     5341 tttgagatca ataataacaa aagattttaa gagccggttg taaaacacaa ggtcaatata
     5401 aaagtgtctt tcatcaaaag atattcgttt ctgtcttgca acaaatgtaa atcctttgcc
     5461 aagttccaga agaaatttct gtaaattatc aattatcgcc tgctcaaatt ctgtttccga
     5521 atattcactc ttttcttcaa gtccgagaaa ttcaagtata tacgggtctt tcacgacatc
     5581 ttcagctttt gtaagaagtt gtcctttttg agctaattca aaaactttgt ctttgtcttt
     5641 gcttagagca agtctttcaa aaagagcaga gtccatttgt cttttgagtt cgcgtactga
     5701 ccagctttcc ttttttgttt cgatttcata aaaactgcgt ttcagttcgt cgtcaattcg
     5761 aataagcata agataatgag tccatgataa atcgaataaa tccaagctgt tgcttgattg
     5821 gctaatcagt gattggcttt ttgttttttc tctgtaagtg ataaaaaact gcttcatgtt
     5881 tttgagatta ctaagagaaa aaccattccc gaattctttt gaaagcttta aagagagttc
     5941 ttttaatgtt tctttcccat attcagctct gtcttttcca ccctgttcgt gttcaacgat
     6001 gatgcgtcca atttcgaaat atgttgaaac catagtggtg tttacggttt ttattacctg
     6061 agatttagct tcatttatga tcctgcttat attctcaaaa agcatatctt ctcttttttc
     6121 gatctgttgt ttcataatac ctctcctatc tcagttctct aactttaatt ctgccgagtc
     6181 cgaaacctgc attttttcca aaatgaaaaa gttcgccagc ttcaaggagc gacctttcaa
     6241 atggagtaaa gcttcctgac agctccatgg ttccttttat tccgccaaga agcattgttg
     6301 ttttctgtct tcccgaccat cttttatttt caacccatct tatgtctgat gaaacaagtt
     6361 tttcatcaat atcctttatt tcctttattt ccggaacagg ggatgattcc ccatacattc
     6421 cgcttaaaat ttcaaccctt ctcctaagac ctttaaggat atcggcatag ctgatcttat
     6481 caacatattg gccattcttt ttaaatcgca aaggtgaaat gaattcaata atatacttct
     6541 tttttgctcc ttttcctgaa tttgtt
//
LOCUS       DAOOYB010000158         6556 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_13431_length_6556_cov_10.647593, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000158 DAOOYB010000000
VERSION     DAOOYB010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6556
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13431_length_6556_cov_10.647593"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..959)
                     /locus_tag="PL056_09390"
     CDS             complement(<1..959)
                     /locus_tag="PL056_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018946674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HPM47272.1"
                     /translation="MKKVFVALIVLTSFLSAQEELPPSTAEEVPVPSFINLENADLSA
                     APEKHEVIKGDTLWDISNTYLKSPWYWPKVWSMNPQISNPHLIYPGDMISFRFTGEMT
                     LPAGEGVGSAEGQGSGVEGSEFEDEGETDVLARTGKTLDATPENFKEYVKLGGKYRID
                     RFKQIDDTVFDVVTKGFIEKGKFEDLGKIVGSFESKELLSTEDNVYVKLGKVSFEVGE
                     YVEFINVKEQIKHPVTKKKVGSIIDIVGRGKVLDVNADRIATVKIVKAFDSIDRGYLV
                     REYIKTEPNIKIAETASDIKAVILTGYDPTAFYGPSYIVYLDKG"
     gene            complement(1041..1643)
                     /locus_tag="PL056_09395"
     CDS             complement(1041..1643)
                     /locus_tag="PL056_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47273.1"
                     /translation="MKKKIFAALIVLLFAAVAVTGCNNNKKDTPPHASDYKESALKYF
                     PLALGNKWTYNVNYFGSAGTIEVVITGTDGDWFLDNRGGRIMADRRGIRDADRYMLMF
                     PLQRESWVSIIDTKTRETRKTVGVDETVAVPAGTFEGAIKVHTYVELPEGRLLHSFHY
                     FVADVGIVKIETAIEEPKEGKMIPQTVTELVSYLINKPKS"
     gene            complement(1640..2287)
                     /gene="lipB"
                     /locus_tag="PL056_09400"
     CDS             complement(1640..2287)
                     /gene="lipB"
                     /locus_tag="PL056_09400"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:TIGR00214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016979 - lipoate-protein ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HPM47274.1"
                     /translation="MKYVIMKGFRDYGTMLALQEKTREEIVAGDHQGVIYFLEHAPVY
                     TSGLRGTEDQILKPLNGVPVFNIRRGGELTWHGPGQLVVYPVIDIRYAGFASIREFVN
                     FFGTAIEKVLSGFCGIENAKWNEEKAGVWVDDRKIAFSGLHFRKFIPIHGYSVNIYSD
                     IQFFNSIIPCGMPNCRITSAAIETGREFSIEDVAEKIAGIIKEKIPCLEKSKEVE"
     gene            complement(2289..3038)
                     /locus_tag="PL056_09405"
     CDS             complement(2289..3038)
                     /locus_tag="PL056_09405"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HPM47275.1"
                     /translation="MRIAVLGDIHSNLPALEAVMKEIKNMSVDAVFFSGDAVGYGPWP
                     GETIDILRENVTAGVLGNHDAGIVGKTDITDYYDAVRYVINWTIDTLNNDQFGFLDRL
                     KYIHSEEKHGSFLLSHGSPRMPERYDYIFNCKGINKLYEVKTYLKNINFIGHSHYMNL
                     FVMTGAEKSVELNISQGEVDVKFPNPVICVCGSVGQPRDGDPRAGFVIYDTEKIKVSF
                     HRVEYDIEKTVQKIKSLRLPNTYGERLRNGY"
     gene            complement(3040..4368)
                     /locus_tag="PL056_09410"
     CDS             complement(3040..4368)
                     /locus_tag="PL056_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HPM47276.1"
                     /translation="MTKKIFVLDTNVILSDPKAIFNFDEHDIVVPIVVIEELDKFKKD
                     MNQIGLSARAFARHIESLRKKGKLVEGVELESGGTIRIDITGDVDLPGPKLNNSYADN
                     IILASAYKITQENPERDVTLVSKDVNLRIKADVLGIKADTYRNDTVNFTKLPRGYENL
                     TVKGDLIDRIFGPEKKISLDELAGTPFEQAHSNKFFMLTDEANEQHQGVVRIDPFENC
                     IVPLKQFKEVWGLRARNFAQKLALDLLMDDRIKLVSLMGTAGTGKTLLAIACGLQKCL
                     DEGKYTKMLVARPIYPMGRDIGFLPGTIDEKLKPWMQPVFDNLEYLLAGYDSSSFGKM
                     NDLLSSGVIEVEALTYIRGRSIPEQFIIVDEAQNLTPHEVKTIVSRVGENTKIVLTGD
                     PFQIDNPYMNESNNGLSYIVDRFKNLPLAGHITLEKGERSALANAAAELL"
     gene            complement(4368..5858)
                     /locus_tag="PL056_09415"
     CDS             complement(4368..5858)
                     /locus_tag="PL056_09415"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HPM47277.1"
                     /translation="MATNMSMEIEEKYIGAVINQRYKIIKKIGVGGMGNVYLAEHEIL
                     KKKVAIKILHFEQSKRKDTLERFKREAIAASNMGQANIVDVTDFGYTEEGNAYFVMEY
                     IEGNSLAEVIKNEKRLPLEYAVSVACQISVALYSAHSKGIIHRDLKPENILITEKDGV
                     TPFVKIVDFGISKIVHDEINKKDRPKTLTKSGSIFGTPEYMSPEQAGGNNVEPTSDIY
                     SLGVIIYEMLTGRIPFSDDNYMKVLHKHQYEFPQIPSSLNPSIPSDMDSLIMKCLEKK
                     PFNRYESMLSLLSDLKKIYIAHNLEKKLSLAFIFNTGTVSGQSGRANAEEGLSGQDII
                     RLLNSGSMTDDTTPEEKRTGTALKITAFLFLSILAGFLIYSFIVNKKGPMIVERETVD
                     RDELEKEQVKDDPPPQKKKPEPVIRKKPPTIKLIVNSNVKGVRVYDKDKGKTICTAPC
                     TKRLPKKDGDVLLLGFEFEDYNIKPMAITLDKDMTVNLNLTTSGGE"
     gene            6040..>6556
                     /locus_tag="PL056_09420"
     CDS             6040..>6556
                     /locus_tag="PL056_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HPM47278.1"
                     /translation="MDRFVAMALLEKHLSNKNLIKHCIATETAMGFFARHLGEDETRW
                     SLAGLLHDLDYDYTKDDFSKHGKMSGEILRKEGFDDTEILDAIVMHSGNVPATSEMGK
                     ALYAVDPATGLVTAGVFMSPEKNIHKMGIDFLIKRFREKRFAAGANREQIMTAATNFD
                     VTLEQFLEIVLN"
BASE COUNT         1731 a         1491 c         1282 g         2052 t
ORIGIN      
        1 cccttgtcaa gataaacaat atagctcgga ccgtagaaag cagtcgggtc gtaaccggta
       61 aggatcacgg cttttatgtc agatgcggtt tcggcgattt ttatattagg ttcggttttg
      121 atgtattccc ttaccagata acctctgtca atggaatcaa aagctttaac tattttgacc
      181 gtggcaatcc tgtcagcgtt aacatccaga acttttcctc ttcctacgat atcaattatt
      241 gaaccgactt tctttttggt cacaggatgc ttgatctgtt ctttaacatt aataaattca
      301 acatattctc cgacttcaaa agaaaccttg ccgagtttca catacacatt gtcctctgtt
      361 gaaaggagct ctttggattc aaatgaaccc actatctttc caagatcttc aaacttccct
      421 ttttcgatga aaccttttgt tacaacatca aaaacagtgt catcgatctg tttgaaacgg
      481 tcaattctgt attttccgcc aagtttgaca tattccttga aattttcggg agttgcatca
      541 agagtttttc ccgttctcgc aagaacatcg gtttcccctt catcttcaaa ttcactgcct
      601 tcaacaccgc tcccctgtcc ttctgccgat ccaacccctt caccggcagg aagagtcatt
      661 tcaccggtaa aacggaaaga gatcatatct cccggatata tgaggtgagg attgctgatc
      721 tgagggttca ttgaccacac tttaggccag taccaaggac ttttcaaata ggtgttgctt
      781 atgtcccaga gagtatcccc ttttatgacc tcgtgctttt ccggagcggc actcaggtct
      841 gcattttcaa gatttatgaa agaaggtacc ggaacctctt cggctgttga aggtggaagc
      901 tcctcctgtg cagaaagaaa cgatgtcagg acgatcaacg ccacaaatac ttttttcatt
      961 cttatctccc gaattttgtc aaaaagtata ttaattatac aacaattacg catacaatac
     1021 tacacttaag cataagtttg tcaacttttg ggcttattta ttaaataact caccagctct
     1081 gtcactgtct gagggatcat tttcccttct ttcggttctt ctattgcagt ttcgatcttt
     1141 acgatcccca catcggcaac aaaataatgg aacgaatgta acaacctgcc ttccggaagt
     1201 tcaacataag tgtgtacctt tattgcacct tcaaaagttc ccgcaggaac cgctaccgtt
     1261 tcatcaacac cgaccgtctt tctggtctca cgggtcttgg tatctataat tgacacccac
     1321 gattctcttt gaagagggaa catcagcata tacctgtcgg catcccttat gcctcgtctg
     1381 tcagccatga tcctgccgcc tctattgtca aggaaccagt ctccgtccgt accggtgata
     1441 acaacttcaa tagttcctgc agaaccgaaa taattgacat tgtaggtcca tttgttaccc
     1501 agagcgagcg gaaaatattt aagtgccgat tctttataat cagaggcatg cggcggagtg
     1561 tcttttttgt tgttattaca acctgttaca gcaactgctg caaataaaag cacaatcagt
     1621 gccgcaaata tcttcttttt cattcaacct cctttgattt ttccagacaa ggtatttttt
     1681 ctttaattat tccggctatt ttttcagcaa catcttctat gctgaactct cttccggtct
     1741 caattgcggc agaggttatt ctgcaattcg gcattccgca tgggattatg ctgttgaaaa
     1801 actgtatgtc actgtaaata ttaactgaat atccgtgtat cggaataaat tttctgaaat
     1861 ggagacccga aaaggcaatt tttctgtcat caacccatac ccctgctttt tcttcattcc
     1921 attttgcgtt ttcaattccg caaaaaccgg aaagaacttt ttcaatggca gtgccgaaaa
     1981 aatttacaaa ctccctgatc gaggcaaatc cggcatatct gatatcgata acaggataaa
     2041 ctacaagctg tccgggccca tgccatgtca attcaccacc tctcctgata ttgaacaccg
     2101 gaacaccgtt aaggggtttt aatatctgat cttcagttcc tctcaggcct gaagtgtata
     2161 caggtgcatg ctcaagaaaa taaataacgc cctgatgatc gcctgcgaca atttcctctc
     2221 gagtcttttc ctggagagca agcatcgttc cataatcacg gaaccctttc ataattacat
     2281 atttcatttc aataaccgtt tcttaatctt tcaccataag tattcggaag tcttaagctt
     2341 ttaattttct ggacagtttt ttcaatatca tactcaactc tgtgaaaact caccttgatc
     2401 ttttctgtat catagattac aaaaccggct cgcggatctc catctctggg ctgacctaca
     2461 gagccgcaga cgcatatcac cgggtttggg aatttgacat cgacctcacc ttgagaaatg
     2521 ttcagctcga cagatttttc ggccccggtc attacaaaaa gattcatgta gtggctgtgg
     2581 ccgataaaat ttatattttt gagatatgtc ttgacttcat aaagcttgtt tatcccttta
     2641 cagttgaata tgtagtcgta cctttcaggc attctcggag atccgtgtga aagaagaaaa
     2701 gatccgtgtt tctcttcgga atgtatgtat ttcaaccggt caagaaatcc gaattgatcg
     2761 ttgtttaaag tgtctatcgt ccagtttatg acatacctca ccgcatcgta gtaatcggtg
     2821 atatcagttt tgcccacaat cccggcatca tggttgccaa gaacccctgc cgtcacattt
     2881 tctctaagaa tatcaatggt ttcaccgggc catggaccgt aacctacggc atctcctgaa
     2941 aaaaatacag cgtccacgga catgttcttt atctctttca tcaccgcttc aagggcggga
     3001 agattggaat gaatgtctcc gaggacagcg attctcatgt cacaaaagct ccgcggcggc
     3061 gttggcaaga gcagaccgtt cgcccttttc aagtgttatg tgtcctgcga gagggagatt
     3121 tttaaaacgg tcaacaatat agctcagacc gttgttcgat tcattcatat agggattgtc
     3181 tatctggaag ggatcgccgg taaggactat ttttgtgttc tcacctactc tcgaaactat
     3241 cgtctttact tcatgcggag taaggttctg cgcttcgtca actattatga actgttcagg
     3301 aatgcttctt cccctgatat atgtgagagc ttcaacttcg atcacaccgc tgctgagaag
     3361 atcgttcatt ttgccgaaac tcgatgaatc gtaacctgcg agaagatatt caagattgtc
     3421 aaagaccggc tgcatccacg gtttaagttt ttcatcaatt gtgccgggca ggaaaccgat
     3481 atcgcggccc atcggataga tcggtctggc aacaagcatt tttgtatatt tcccttcgtc
     3541 aagacacttc tgaagaccgc atgcgatcgc aagaagggtt ttccccgttc cggctgttcc
     3601 cattagtgat acaagtttga tcctgtcgtc catcagaagg tcaagtgcca gtttctgtgc
     3661 aaaatttctc gccctcaaac cccagacctc cttaaactgc ttaagaggaa cgatacagtt
     3721 ttcaaacgga tctatcctca cgacgccctg gtgctgctca tttgcttcat cggtaagcat
     3781 gaaaaacttg tttgaatgtg cctgctcaaa gggggttccg gcaagttcat caagagagat
     3841 cttcttttcc ggtccgaaaa tcctgtctat caggtcgccc ttaacagtca ggttttcata
     3901 acctctggga agttttgtga aatttactgt atcgttgcgg taagtgtctg cttttattcc
     3961 cagaacatcc gcctttatcc tcaggttgac atccttgctg acaagcgtga catctctttc
     4021 agggttttcc tgtgtgatct tgtatgccga tgccagaatt atgttgtcgg cataactgtt
     4081 attgagtttt ggtcccggga ggtcaacatc tccggttata tctatcctta tcgttccgcc
     4141 actttcaagc tcaacgcctt caacaagttt ccctttcttt ctcaatgatt caatgtgtct
     4201 tgcaaaagct ctcgcggaaa gacctatctg gttcatgtcc tttttgaatt tatcaagctc
     4261 ttcgatgaca actatcggaa caacaatgtc atgttcatca aaattaaaga tcgctttcgg
     4321 gtcggaaaga ataacattgg tgtcaagtac gaagatcttt tttgtcatta ttcgcctccg
     4381 gatgttgtta aattcaggtt taccgtcata tctttatcaa gcgttatagc cattggcttt
     4441 atgttgtagt cttcgaactc aaatccgaga agaaggacat ctccgtcctt tttggggagc
     4501 cgttttgtac aaggtgctgt acagatggtc tttcctttat ccttgtcata aacacggacc
     4561 cctttaacat tcgagttcac gatcagtttt attgtcggcg gtttctttct gatcactggt
     4621 tcaggtttct ttttctgagg cggcggatca tctttcacct gctctttttc cagttcatct
     4681 ctgtcaacgg tctccctttc aactatcatc ggcccttttt tgttgacaat gaagctgtag
     4741 ataagaaatc ctgcaagtat cgaaaggaac agaaatgccg ttatcttaag tgccgtaccc
     4801 gttcttttct cttcaggtgt cgtatcatcg gtcatcgatc cggaattaag aagtcttatt
     4861 atgtcctgtc cggaaagtcc ttcctccgca ttggcacgac cactttgacc tgaaacagtt
     4921 ccggtgttga aaataaacgc cagcgaaagc tttttttcaa ggttgtgagc gatatatatc
     4981 tttttgagat cgctgaggag tgaaagcatt gattcatatc tgttgaaggg ctttttctca
     5041 agacatttca ttatcagcga atccatatcg gaagggatgg atggattgag cgatgatggt
     5101 atctgcggga attcatactg atgtttgtgc agaactttca tataattgtc atcggaaaaa
     5161 ggaattcttc ctgtcagcat ttcatagatt atcacgccga gagaatagat gtcagaagtg
     5221 ggttctacat tgtttccgcc tgcctgttca ggtgacatat actccggtgt tccgaagatg
     5281 gaacccgatt ttgtcagtgt cttggggcgg tcttttttat ttatttcatc gtgaacgatc
     5341 ttggatatcc cgaaatcaac aatttttacg aacggggtaa cgccgtcttt ttccgttatg
     5401 agtatgttct ccggcttaag gtcccggtgg attatcccct tgctgtgagc agaataaagt
     5461 gcgactgaga tctgacaggc gacagatacc gcatattcaa gaggaagccg tttttcattt
     5521 ttgataactt cggcaagaga gtttccttca atatactcca tgacaaaata tgcgttcccc
     5581 tcttccgtat aaccgaaatc tgtgacatca acaatatttg cctgacccat gttggaagcg
     5641 gctatcgcct ctcttttgaa ccgttcgagt gtgtcttttc ttttgctctg ctcaaaatga
     5701 aggatcttta tggcgacttt ctttttaagt atctcgtgct cggcaagata gacatttccc
     5761 atgccgccga caccgatctt tttgattatt ttatatctct gatttataac agcgccgatg
     5821 tacttttctt caatttccat actcatattt gtagccaaaa caacctctct gcaaaaatta
     5881 cccgaactca aaaaagaaac gggtacaaga ttagcagtta cataaaataa agtcaattta
     5941 taataacact gcttacttaa aaaatacata cccataatat tgacaaactc actaaaaaaa
     6001 cataaccagt ttaaaatgga ttttttaaag gaggctcctg tggatagatt tgttgctatg
     6061 gcccttttgg aaaagcatct ttccaacaaa aatcttatca agcactgtat tgcaactgag
     6121 accgcaatgg gattttttgc ccggcatctt ggagaagatg aaaccagatg gtcacttgca
     6181 gggcttctgc acgatcttga ttatgattat acaaaggatg atttttcaaa acacggaaaa
     6241 atgtcaggcg aaatattgag aaaagaagga tttgacgaca cggaaatact tgatgcgata
     6301 gttatgcatt cgggaaatgt ccctgccact tcagaaatgg gaaaagcgct ttatgcggtt
     6361 gatccggcaa caggtcttgt cactgccggt gtttttatga gccctgaaaa gaatatccac
     6421 aaaatgggta ttgattttct cataaaaagg ttcagggaaa aaagatttgc cgccggagcc
     6481 aaccgtgaac agataatgac agccgcaacc aatttcgatg tgactcttga acagttcctc
     6541 gaaatagttt taaacg
//
LOCUS       DAOOYB010000159         6505 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_13588_length_6505_cov_11.153643, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000159 DAOOYB010000000
VERSION     DAOOYB010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6505
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13588_length_6505_cov_11.153643"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="PL056_09425"
     CDS             complement(<1..653)
                     /locus_tag="PL056_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014395501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HPM47279.1"
                     /translation="MAGKTAVCIPTYNEKENIVKIMEAVHKEIPVADIFVVDDNSPDK
                     TAEIVNEFMKHNQYVSMKVRNSKEGLGAAYVDGFTTLIERGYEVIIQMDADFSHQPHY
                     LPEMIKKIDEGFNVVIGSRYVKGGGTRNWNIMRRIISRGGSFYASTILNLGVHDVTAG
                     FKCWNAKILKKVISTPLVLSGFGFQIEMAFRTKVAGAKVYELPIVFPDREEGESKMSG
                     "
     gene            complement(655..2286)
                     /locus_tag="PL056_09430"
     CDS             complement(655..2286)
                     /locus_tag="PL056_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47280.1"
                     /translation="MKIVSVFLKNFLIIKKSEIELSGGLTTVTGETGSGKSLFVSAMK
                     ALRGERIGRSFLGRWGDTGEVTAEIALEECDSDIKKGLQSGGIDLEEGEPLVLRRLFG
                     EKNGSYINDSPVSASFLNEIFSDHIEIGSQFENRELFKKDYRMRIVDLKADNMKKLAE
                     YRAIFEKISECRHEMAAFVKKDDPARREYLEYQISELEALQTYEGEDEKLQSRMKFIE
                     NRAKIIKLGSELSEFIENSSKDLAKCDTIASQLEKLIDIKELSERISAASIETDDISR
                     SVSAHLSKYDEDDENVEDVEKRFNLLSALMIKHGVQSSAGLVEKMNSMSDELFDMDKV
                     PQKIKELEKELQTLIKKGMEKALQLRTGREKAARKIEENILKYLDRFGMSGVRFSVSL
                     NYIKDLNETGLDEVEFSVNTIGTEKMFDISSLSGGELSRLLLAVKLVDDEEGRVLLFD
                     EIDSSIGGETAKSAAGEMKKNSEKNQIIVVTHFPQTAAVAHRHILVEKSVTDGEITAG
                     IKDLGHDEKIKELARMMGDSDSGVFLKTAEKMIGG"
     gene            complement(2288..3490)
                     /locus_tag="PL056_09435"
     CDS             complement(2288..3490)
                     /locus_tag="PL056_09435"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HPM47281.1"
                     /translation="MRNDFLEKLFKRKGGIKLGLGRVAGAFPSVLEKRIPVYHVAGTN
                     GKGTAVYSIAHTLEKNGFRTGRFISPHLFDFNERISVDGIDISDAKVRELYEFLEKKI
                     EGFEELSFFEVTFLMAWKHFELENCDRVVLEVGLGGRLDATNVINWPKTDIITSIGLD
                     HTHILGDTIEKIASEKLGIIKQGDTVVIGSDFSVGAKNLLPLHEWLKEEAILRGAETV
                     TDENLCGNLETNRDDLSPDQIKNIKLAVSAVQITEKNIAVPDFSQMVLPGRFQLIEPD
                     VRIDVAHNPPAMRSLVEHAVKKGIRPVVLFGAMKDKNIEAVLNELARIAVEIYVITVE
                     EENRGASADEIILRSSEYAEKIIKKSQNDEETMKNALLSAHANNTELLVTGSFYTIEK
                     FVKRRKNL"
     gene            complement(3487..4422)
                     /locus_tag="PL056_09440"
     CDS             complement(3487..4422)
                     /locus_tag="PL056_09440"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPM47282.1"
                     /translation="MKIISFNIDVDPLINYYSIHGLGSHDTFPDPVYECGIKRFLELF
                     DRYGIKATFFITAGGLNGAVPGILRNIAEKGHEIGNHSYSHDYRLTLMNDDEIFEDIR
                     KNHEAIKDLTGFECLGFRSPGYNSSGAIISALKKMSYSYDSSLFPSFSYYLAKWLLIN
                     LKMLAGRRSRSVISSFLDAFGFYKPEFIDENIKDVKKTGALVEIPITTLFPPAGIPLI
                     GTSLICFPKWLINLMLKSSLKRDIVNIEFHGIDLVEVNDSDNLEPLKSMQPDLKYDLA
                     HKLQRFEQVIRFYTENGYRFMTLSEIADMKKRFEL"
     gene            complement(4422..5141)
                     /locus_tag="PL056_09445"
     CDS             complement(4422..5141)
                     /locus_tag="PL056_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013938551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPM47283.1"
                     /translation="MGKRKVSIVIPVYNEEKILETAVNRLKKDLDQFRDEFDFEIILA
                     ENGSTDRTIEKGGELSKIDSSVSIFHYEHPDYGLALQEGIRRAGGEIIVCDEIDLCLP
                     EFYEKALAEFKNSDAQMVVGSKRAKGANDQRPFKRRFATWVLNVMLKIILGFKGTDTH
                     GVKAFVKKPLMPVLDKCLVGKDIFASEFVIRAERMGIKVKEVPISLDEKRAPSIKLMK
                     RVPKVLSNLAYLFWNIRIRNR"
     gene            5401..5934
                     /locus_tag="PL056_09450"
     CDS             5401..5934
                     /locus_tag="PL056_09450"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPM47284.1"
                     /translation="MKDSIRKRIIEKARDLLFTKTEEEITMNLIANELNITAPTLYHY
                     FKGKDELLGAANQLITEEICAHLGIKFPPSIPSEMRIITATSMIAEYFVKTGLPVSYL
                     VEDPKDRPIVLKEFRKKFTEMFSNYLKTKKPGSKVGAEQTTMRYLSILQADLAYIRNN
                     KKELSEDFAEKVFSVLF"
     gene            6035..6463
                     /locus_tag="PL056_09455"
     CDS             6035..6463
                     /locus_tag="PL056_09455"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HPM47285.1"
                     /translation="MDNRRKKEDRRDDIQFKLPKDDRGKSDRRGRERRTKERIPVKMW
                     IRNIDGNANYFQQTGNLSVSGMYIMSPNPYPENTTIEVEFQVPGTDHIVRCHAQVLKC
                     NPDGQFMGLSVKFVNMAEKDRQIVGKAVDELISEFWYIME"
BASE COUNT         1826 a         1478 c         1117 g         2084 t
ORIGIN      
        1 ccgctcattt tgctttcccc ttcttctcta tccgggaaaa caatgggcag ttcgtaaact
       61 tttgctccgg caacttttgt tctgaaagcc atttctatct ggaaaccgaa accggaaagg
      121 acaagcggcg tgcttataac ttttttaagg atctttgcat tccagcattt gaaaccggca
      181 gtaacatcgt gaactccgag attgagtatg gttgacgcat agaaacttcc gcctcttgaa
      241 atgatccttc tcatgatgtt ccagttcctg gtgccgccgc cttttacata tcttgaaccg
      301 atcacaacat tgaaaccttc gtcaattttc ttaatcattt caggaagata atgcggctga
      361 tgtgaaaaat cggcatccat ctgtattatc acttcatagc ccctttctat aagcgtggtg
      421 aacccgtcga cataggcggc acccaaccct tcctttgaat ttctcacctt cattgagaca
      481 tactggttat gcttcatgaa ttcattaacg atttcggcgg ttttatcagg cgagttatca
      541 tcaaccacaa aaatatccgc aacaggaatt tccttgtgta ctgcttccat tattttgact
      601 atgttctctt tttcattgta tgtcggaata cagactgctg ttttccctgc cattttaacc
      661 tcctatcatt ttttccgccg tcttgaggaa aacaccggag tcgctgtcgc ccatcattct
      721 tgcaagttct ttaatttttt catcgtgtcc aagatcttta attcctgctg ttatctcacc
      781 gtcagtcaca cttttttcaa caagtatgtg tctatgtgca actgccgcgg tctgtgggaa
      841 atgagtgact acaattatct gatttttttc agaattcttt ttcatttcac ctgccgcact
      901 ttttgctgtt tccccgccta ttgaactgtc tatctcgtca aaaagaagca cacgcccctc
      961 ttcatcatca accagtttta ccgcaaggag aagccgtgaa agttctccac ctgaaagaga
     1021 agagatatca aacatctttt ccgttcctat cgtgttaaca gaaaactcaa cctcatcaag
     1081 accggtttcg tttaaatcct taatataatt caatgaaaca ctgaatctta caccgctcat
     1141 gccgaatctg tcaagatatt ttagaatgtt ctcttcgatc tttcttgccg ctttctctct
     1201 tccggtcctt aactgaagtg ctttctccat cccttttttt atgagtgtct gcaactcctt
     1261 ttccagctct tttatctttt gaggaacttt atccatatcg aaaagttcat cgctcattga
     1321 gttcatcttt tcaacaagac ctgctgaaga ctgaactcca tgcttgatca tcagcgcaga
     1381 aagcaaatta aacctttttt cgacatcctc gacattttca tcatcctcat cgtattttga
     1441 caagtgtgcg gaaacacttc ttgaaatgtc atcggtttcg atcgatgctg cacttattct
     1501 ttcagagagc tccttaatgt ctatcaactt ttccagttga gaggcgattg tgtcgcactt
     1561 ggcaagatct ttcgaagaat tttcaataaa ttcagagagt tcagaaccaa gctttataat
     1621 tttcgccctg ttttcaatga atttcatcct ggactgaagt ttttcatctt caccttcata
     1681 ggtctgaaga gcttcaagtt cacttatctg atattcaaga tattctcttc ttgcaggatc
     1741 atcttttttc acaaatgcag ccatttcatg tctgcactca cttatttttt caaagatcgc
     1801 cctgtattca gcaagctttt tcatattgtc cgctttcaga tcaacaattc tcatccggta
     1861 atccttttta aaaagctccc ggttttcgaa ctgacttcct atttcgatat gatctgaaaa
     1921 gatttcgttt aaaaacgatg cgctgacagg cgaatcatta atgtatgaac cattcttttc
     1981 gccaaaaagt cttcttaaaa caagcggctc cccttcttca agatcaattc ctccgctttg
     2041 cagacctttt tttatatcgc tgtcacactc ctcaagggct atttcagccg taacttcacc
     2101 tgtgtcaccc catctgccga gaaaactcct gcctatccgc tcgcctctta aagctttcat
     2161 cgctgaaacg aaaagacttt ttccgctgcc ggtctctcct gtcacagtcg taagtccgcc
     2221 tgaaagctct atttccgatt tttttatgat gagaaaattc ttgagaaaaa cagaaactat
     2281 cttcatttta caagtttttc ctccgcttca caaacttttc aattgtgtaa aaacttccgg
     2341 ttacaagtag ttcagtgttg tttgcatgtg ctgacaaaag agcgtttttc attgtttctt
     2401 catcattttg cgactttttt attatctttt cagcatattc ggacgatctt aatattattt
     2461 catccgccga agcccctcta ttctcctctt caaccgtaat gacatatatt tcaactgcaa
     2521 ttctggcaag ctcattcaga accgcttcaa tatttttgtc tttcatggca ccaaacagaa
     2581 caaccggtct gatccccttt tttacggcat gttcaacaag cgatctcatc gcaggaggat
     2641 tatgggcaac atcaattctc acatccggtt cgatcagctg gaatcttccc ggaaggacca
     2701 tttgtgaaaa gtcaggaaca gcaatgtttt tttctgttat ttgaacagcc gacactgcaa
     2761 gttttatgtt ttttatctgg tcagggctta gatcatcacg atttgtctca agatttccgc
     2821 ataaattctc atcagttacg gtttcagctc ctcttaaaat agcttcttct ttcaaccatt
     2881 catgtaaggg caaaagattt tttgccccta cggaaaaatc acttccgatt acgaccgtgt
     2941 caccctgttt aatgattccg agtttttcag aagcgatctt ttcgatcgtg tctcctaaaa
     3001 tgtgggtatg atcaagtcct attgaagtta ttatgtcagt tttcggccaa tttataacat
     3061 ttgtcgcatc aagccgcccg ccaagcccca cctcaagaac aaccctgtca cagttttcaa
     3121 gttcaaaatg tttccacgcc attaaaaatg tcacttcaaa gaatgagagt tcttcaaatc
     3181 cttctatctt tttctcaaga aattcataca gttcccggac ttttgcatca cttatatcaa
     3241 ttccgtcgac tgatatcctt tcattgaaat caaataaatg cggagatatg aatcttccag
     3301 tcctgaagcc gttcttttca agggtatgag cgatcgaata gactgccgtt ccttttccgt
     3361 tcgtgcctgc gacatgataa accggtattc tcttttcaag aactgaagga aaagcacctg
     3421 caactctgcc gagaccgagc ttaatccccc cttttctttt aaaaagtttt tcaagaaaat
     3481 catttctcac aattcaaacc tttttttcat atctgcaatt tccgacaggg tcataaacct
     3541 atatccattc tcagtataaa atcttataac ttgttcgaat ctttgaagtt tatgtgcaag
     3601 atcgtatttc agatcgggct gcatactctt caacggttca agattatcac tgtcatttac
     3661 ctcaacgaga tctatgccgt gaaactcaat gttcacaata tcccttttca atgaactttt
     3721 taacattaaa tttatcagcc acttcgggaa acagatgaga gaagtgccga tcagaggaat
     3781 tccggcagga ggaaaaagtg ttgttatcgg gatctccaca agtgcccctg tcttcttcac
     3841 atcttttatg ttttcatcaa tgaattcggg cttataaaaa ccgaatgcat ccagaaatga
     3901 agatattaca gatcttgatc tccggcctgc gagcatcttt aaattgataa gcagccattt
     3961 ggcaagataa tacgagaatg acgggaacag cgaagaatcg taagaataac tcatcttttt
     4021 caatgccgaa attattgccc ctgaagagtt gtatccggga cttctgaacc ctagacactc
     4081 aaaacctgta agatctttta ttgcttcatg gttttttctg atatcctcaa atatttcgtc
     4141 gtcgttcatc agtgtaaggc ggtaatcgtg agaatacgag tgattcccta tttcatgccc
     4201 tttttctgca atatttctca aaataccggg cactgctccg ttcagtcctc cggctgtgat
     4261 gaaaaaagtc gcttttattc cgtatctgtc aaaaagctca aggaaccttt ttatcccgca
     4321 ttcatataca ggatcgggaa atgtgtcatg agaaccgagt ccgtgaattg aataataatt
     4381 gatcagcgga tcgacatcta tattgaaaga aatgatcttc attaccggtt tcgtatccgg
     4441 atattccaga aaagataggc aagatttgaa agtactttag gcaccctttt catcaatttt
     4501 atcgacgggg ctctcttttc atcaagagat atcggcacct cttttacttt tatccccatt
     4561 ctttcagccc tgatgacaaa ttcactcgca aaaatatcct tgccgacaag acatttgtcg
     4621 agcacaggca tcagcggttt ttttacaaat gccttaactc cgtgagtatc ggtccctttg
     4681 aaaccgagaa ttattttaag cataacattg agaacccatg tggcgaacct tctcttaaaa
     4741 ggccgctggt cattggctcc cttcgctctt tttgacccga cgaccatctg ggcatcgctg
     4801 ttcttgaatt cagcaagggc tttttcataa aattcaggga gacacagatc tatctcgtca
     4861 cagactatta tctcaccccc ggcccgtcgt atcccttcct gaagagcaag tccatagtca
     4921 ggatgctcat aatgaaaaat ggataccgaa ctgtcaatct tagatagttc gccccccttt
     4981 tctattgtcc ggtcagtact tccattttcc gccagtatga tctcaaaatc aaattcatct
     5041 ctgaactgat cgagatcttt tttcaaccgg tttacagctg tttcaagaat cttttcttcg
     5101 ttgtataccg gaattactat tgaaactttt ctttttccca tttcacagac cctataaaaa
     5161 ttctttgaag aatataataa agcgtttgaa gatagtcaaa ttatttaatc aatatagcag
     5221 tgcattttct ttagagaatt ttgtttacat ttaacctttt ttgtgttaat aatggaaatg
     5281 tgttcattta actattaaag aatctgtttt caatttgata taaatagatt ttttgacggc
     5341 tcattaaata ttgtaaaaat ggttttgtaa tattgttttt ccattgtaag gagtaaaaaa
     5401 atgaaagaca gcatcagaaa aagaattatt gagaaagcaa gggaccttct tttcacaaaa
     5461 acagaagaag agatcacaat gaacctcatt gcaaacgagc tgaacattac agctccgaca
     5521 ctttatcatt actttaaagg caaggatgag cttctcggtg cagctaacca gcttataact
     5581 gaagagatct gtgctcacct tgggatcaag tttccccctt caattccgtc cgaaatgcgt
     5641 atcataactg ctacaagcat gatcgcagag tatttcgtaa aaacaggtct ccctgtttcc
     5701 taccttgttg aagacccgaa agacagaccg atcgttctta aggagttcag aaagaaattc
     5761 actgaaatgt tcagcaacta ccttaaaaca aaaaaaccgg gatcaaaagt cggtgcagaa
     5821 cagacaacaa tgcgttatct ctctatcctt caggcagatc ttgcatacat cagaaacaac
     5881 aaaaaagagc tttccgaaga tttcgcagaa aaagttttct ctgtcctttt ctaaaaaata
     5941 caacaatcaa cccccgataa actcataaaa ttggactttc cctttccaat taattatatt
     6001 tccgtgtaat tttgaatttt tcggaggtac ttaaatggac aacagaagga aaaaagaaga
     6061 ccgcagagac gatattcaat tcaagctccc caaggatgat aggggcaaat ctgacagaag
     6121 aggcagggaa agaagaacga aggaaagaat acctgtcaaa atgtggataa ggaatattga
     6181 cggtaatgct aactattttc agcagaccgg caatctgagt gtaagcggca tgtacattat
     6241 gtctcctaac ccgtatcctg aaaacactac tatcgaagtt gaatttcagg ttcccggaac
     6301 agatcatatc gtcaggtgcc atgcacaggt tctgaaatgt aatcctgacg gacagttcat
     6361 ggggctcagt gttaaatttg tcaacatggc ggaaaaggac agacagatcg taggaaaggc
     6421 agttgatgaa cttataagcg aattctggta tatcatggag tgacatggac cgtatttaca
     6481 ccgatgttct gattatcgga agcgg
//
LOCUS       DAOOYB010000160         6500 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_13606_length_6500_cov_10.537471, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000160 DAOOYB010000000
VERSION     DAOOYB010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6500
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13606_length_6500_cov_10.537471"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            147..1910
                     /gene="uvrC"
                     /locus_tag="PL056_09460"
     CDS             147..1910
                     /gene="uvrC"
                     /locus_tag="PL056_09460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HPM47286.1"
                     /translation="MNTINAFIIDSDLLKKIPASPGVYQFRDKLGKTIYIGKAKDLKS
                     RIGQYVSRSDSRLMIENLMSSAASVEIILTNSESEALILESLLIKKKRPFFNIDLKDD
                     KSYPYLAVTDEEWPRLIITRHITRKYRYLRGPFTSATLLRSLKSLLQVLYPLKYCSEK
                     NPAGCINFQMGLCPAPCRKNTDKTVYDNNVRNIIEILQGKKWKELNDLIKENIETSSQ
                     VLNFEKAAKLRDLLAMIPEIKKKFSVEFSGTGVSDFFMLRKQGEIQFFAVARYHDGAL
                     NSLRTFTVTAITDEIESSVASALATFYSGNSKAEKIFIYPNVLSNEQIEQVSGFTVKK
                     SGAIPKTIMNILETNIDSAMKNYIRDTQSRGIMLNELSEFVNAEITSIMCLDISTFGG
                     EFTVAGAVWWEKGKFVKNNYRKFRIRTVEGIDDFGSLKEVASRLMKKWKENSFPVPSL
                     LLIDGGAGQISSVYSVVGEVLPIAGIIKDRRNLKGHELLLNSSGEEMLLNDSIFALTM
                     KSIRDETHRFSISFNRNLRKSKLNSALAQIEGIGKSRELALLQHFRTIANIRSATIEE
                     LMEVKGINRKLAQKIHSSLIG"
     gene            complement(1916..2002)
                     /locus_tag="PL056_09465"
     tRNA            complement(1916..2002)
                     /locus_tag="PL056_09465"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1966..1968),aa:Leu,seq:cag)
     gene            2117..2905
                     /locus_tag="PL056_09470"
     CDS             2117..2905
                     /locus_tag="PL056_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47287.1"
                     /translation="MIFTIQFFLLFPALLDQNSILEKASEKGFFYRNADICEVAKCSE
                     TETCVEHSGKNYVIKKKDRFEVSASVSQKAIEIEITPDNSYISYFIYIQHKAGVHRIQ
                     MNKKKLVIDQGVANIIKLQIVGTASTGPKVIWEKPLKKTVPVPFSRSFDESVHNIRKT
                     YFQKPLMISEELKPAAEESLKRLKNEGLVHYSSKTGSVRHTGIDKKVLGENLFIAESE
                     EKAWKMLVNSPSHLYNLINPHFKKYFVLFTKNDEETAGVILFSE"
     gene            2984..3490
                     /locus_tag="PL056_09475"
     CDS             2984..3490
                     /locus_tag="PL056_09475"
                     /inference="COORDINATES: protein motif:HMM:NF013340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HPM47288.1"
                     /translation="MADINLSSMLASSVSMADGGRLPTKFTADGLNISPHIGWQRIPR
                     DTKSIAIIMNDPDSSKGVWIHWGIFNIPSFLIELKENIPPERNLEYNMRQIVNDFGKV
                     GYYGPLNASGKHKYVFTVCALDTIINLPFNPTGEDLIKAMHGHVLEKALLRTHYGSNI
                     IPNTVVKH"
     gene            3602..4588
                     /locus_tag="PL056_09480"
     CDS             3602..4588
                     /locus_tag="PL056_09480"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPM47289.1"
                     /translation="MIVVTGASGHVGNNLVRELNRRKIKPRVVIHNPEHAEKPLKGLD
                     FEAVMGDVTDLQSMIEAFKGAEMVYHCAAWISITPGMYKKLRVVNVEGTLNVLEACRR
                     NHVKKVVYVSSIEALGDPGEGVIAKEEMGFNPDKAMLEYGVTKAEAALLVQEAVKNGM
                     DIVTVCPVGIIGPYDFKSSQMGNMMIDFYKGKLPAYPGYGGFDWVDVRDVANAIILAG
                     EKGKKGESYLLTGGHTTNFEMMEILEKVTGKKKPKIALPYWLMSSAGFFAEFYYKATG
                     KEAVITRDSAKILKSDLRASSEKAKRELGYDPRPLEQTFKDHFEWLIETYGK"
     gene            complement(4590..5768)
                     /gene="xseA"
                     /locus_tag="PL056_09485"
     CDS             complement(4590..5768)
                     /gene="xseA"
                     /locus_tag="PL056_09485"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HPM47290.1"
                     /translation="MMNDVVVPVSALVRDLKHAVEGAFGFVAVRGELTNLSKAYSGHI
                     YFTLKDSEAQIKCALFKNQQKMNHAELKVDSEYIVYGKLSVYEQRSEITLIANLIIPY
                     GEGMAAVKLKMLKEKLSQEGIFDVSHKKQLPQFPHTIGVVTSESGAAIHDIIKVSRKR
                     FPPAQIILSPSSVQGRDAHLDLIKALERMSDIGDIDAIVIGRGGGSKEDLDCFNSEEL
                     ARAVIKCRKPVISAVGHETDTTILDLAASYSVSTPTAAAELIFPDGTVLLDQAANAVL
                     NMKKTVESSINSKLMFLDNMTLSLKNPGEIISQTCHRTEKLLLTAASKIKELIFDAVK
                     DLSALEKELETNNPLSPLERGFAVVTQNDRMIKRKKFLNESGSFSVTFKDGKVFVEHD
                     "
     gene            complement(5765..>6500)
                     /locus_tag="PL056_09490"
     CDS             complement(5765..>6500)
                     /locus_tag="PL056_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47291.1"
                     /translation="MIQDEDLWKMLITHKDFVEFTAPKKDDFVKFFSTYSPVLYDLYI
                     EQVGYVVKDIPAEVESGEKPDEEIKPDEQPGQEDEDVVVLGDEDFDFPDFLVSDEVFE
                     GLNAEDMTVQRKSMMEMIKDMSMGQKVKLAMMGNMEVRKILIKDPRRQIAISVLANAR
                     ITEKEIVSIAADAAVALDIIAYIAAVKSLSKSYQVKLALVNNPKTPLKTSMALLDVIR
                     INDLKNIAKSRNIPNALKMKALKKIR"
BASE COUNT         2053 a         1323 c         1319 g         1805 t
ORIGIN      
        1 aaatacatcg ttaataatcc ggcaaattgg gaaaatgatg aaaattattt atctgtaggg
       61 gcgccccacc gtggtcgccc tgacaaggat ggcgatattc tgggcgagga cagtgcctcg
      121 cccctacgaa ataacggaaa attagaatga acacaataaa cgcgtttatt attgattctg
      181 atctgcttaa aaagatccct gcgagtccgg gtgtttatca gttcagggac aagcttggga
      241 aaacaatcta catcgggaag gcgaaagatc tgaaaagccg catcgggcag tatgtttcaa
      301 ggagtgattc gaggttgatg atcgaaaatc tgatgagtag tgccgcaagt gttgagataa
      361 ttctgaccaa ttcagagagt gaggcgctta ttttagagag tttgctgata aagaaaaaac
      421 ggccgttttt caacatagac cttaaagatg ataagagtta tccgtatctt gctgtaactg
      481 atgaggagtg gccgcgactt atcataacaa ggcatataac aagaaaatac aggtacttgc
      541 gcggcccttt cacttctgcc acacttttaa ggtcgcttaa aagcctgctt caggtgcttt
      601 atccgcttaa atactgttca gaaaaaaatc ctgccggttg cataaatttc cagatgggtc
      661 tttgccctgc tccgtgcaga aaaaacaccg ataaaactgt ttatgataac aatgtcagga
      721 acataatcga gatccttcag gggaaaaagt ggaaggaact taatgatctt attaaagaaa
      781 atattgaaac aagttctcaa gtccttaact ttgaaaaagc ggcgaaactt cgggaccttc
      841 ttgcaatgat ccctgaaata aaaaagaagt tcagtgtcga attttcagga acgggagtga
      901 gtgatttttt tatgctcaga aagcagggtg aaattcagtt ctttgctgtc gcaagatatc
      961 acgacggagc tcttaactca ttaagaacat tcacagttac agcgattaca gacgaaatcg
     1021 aaagttcagt tgcctcagct cttgcaacat tttattcagg aaactcaaaa gctgagaaaa
     1081 tattcatata tccaaatgtt ctaagcaatg agcagattga gcaagtgtcc ggatttactg
     1141 ttaaaaaaag cggagctatt ccgaaaacca ttatgaatat ccttgaaacc aacattgatt
     1201 cagccatgaa aaattatatc agggacactc agagcagggg cattatgctc aatgaacttt
     1261 cagagtttgt aaacgcagaa atcacttcga taatgtgtct tgacatctca actttcggcg
     1321 gagaattcac cgttgccggc gcagtatggt gggaaaaagg aaaattcgtt aaaaacaact
     1381 accgcaagtt cagaataaga actgttgaag gaatagatga tttcggcagt ttaaaggaag
     1441 tggcatcgcg gctcatgaaa aaatggaagg aaaacagttt cccagtccct tcattacttc
     1501 tgatagacgg cggagcaggg cagatttcat cagtatattc agtggtcgga gaagttcttc
     1561 cgatcgcagg aattattaaa gacagaagaa atctgaaagg tcatgaactg ctcttaaatt
     1621 catcaggcga agagatgttg ttaaacgatt caatattcgc tctcacaatg aaatcaataa
     1681 gagatgagac acacagattt tcaatatcat tcaacaggaa tttaagaaaa agcaaattaa
     1741 attctgcact tgcacaaatt gaaggaatcg gaaaaagcag agaacttgca cttttgcagc
     1801 acttcagaac gatcgcaaat atcagatctg caacaatcga agaattaatg gaagtcaaag
     1861 gaataaacag aaaacttgct cagaaaatac attcttcact tatcggataa aaagatggtg
     1921 ccgggaacag gactcgaacc tgcacagagt tgcccccact agaacctgaa tctagcgcgt
     1981 ctaccaattt caccatcccg gcacgataga acgcgctgat tatactcatc tcttttcaga
     2041 gtcaagattt tccctttttc tggcttttga tcttgatgaa agtataatcg ttaaagttcg
     2101 atgagatgag ggaaccatga tttttacaat tcaatttttt ctcctttttc ctgcacttct
     2161 ggatcagaat tccatccttg aaaaagcgtc tgaaaagggg ttcttttatc gcaatgctga
     2221 tatttgcgaa gttgcaaaat gttcagaaac ggaaacttgt gtggaacatt cagggaaaaa
     2281 ttatgtcata aagaaaaaag accgttttga agtttctgca agtgtttcac agaaagctat
     2341 cgaaatagag ataacacctg ataacagcta catatcttat ttcatataca ttcagcacaa
     2401 agcgggtgtt cacagaatac agatgaataa aaaaaagctc gtcatcgacc agggagttgc
     2461 aaatatcatt aaactgcaga tagtaggaac agcttccact ggaccgaaag tcatctggga
     2521 aaaaccgctg aaaaagaccg ttcccgtccc tttttccaga tcttttgatg agtctgtcca
     2581 caacataaga aaaacatatt tccagaaacc gcttatgatt tcagaagaac taaaaccagc
     2641 agcggaagaa tcactgaaac ggcttaaaaa tgagggactt gtccattatt catcaaaaac
     2701 gggaagcgta agacataccg gcattgataa aaaagtgctg ggagaaaatc tttttattgc
     2761 agaaagtgag gaaaaagcat ggaaaatgct tgtaaacagc ccttcgcacc tttacaatct
     2821 tatcaaccct cactttaaaa aatactttgt tttatttacg aaaaatgatg aagaaacagc
     2881 gggcgttata ctcttttctg agtaaaatga cccgtttttc ttgactattt ttttcaaaat
     2941 gtatactatt taaagcattt tgaattaaat agcggagaga gttttggctg atattaactt
     3001 aagttcaatg cttgcaagca gtgtttcaat ggctgatgga ggaagacttc ctacaaaatt
     3061 cactgctgac gggctcaaca tttcacctca tatcggctgg cagagaatac ccagagatac
     3121 aaaatccata gcaattataa tgaacgatcc cgattcatca aaaggagttt ggatccactg
     3181 gggcattttt aacattccgt catttctcat tgaacttaaa gagaacattc ctcccgaaag
     3241 gaacctcgaa tacaatatgc gccagattgt caatgatttc gggaaagtgg gctattacgg
     3301 accgcttaat gcaagcggaa aacacaaata tgtgtttaca gtatgtgcac ttgataccat
     3361 aattaatctt ccctttaacc caacaggaga agaccttata aaagcgatgc acggacatgt
     3421 tcttgaaaaa gcacttttaa gaactcatta cggaagcaac attattccta acactgtcgt
     3481 gaaacattaa gccggacaga atcttacatt tttcattccc gccatcttct taaaaagtat
     3541 aaaacaactt gcaaaaatca gcttttgaaa taatattgag cgttttttca gggagggaga
     3601 aatgatagta gtaaccggtg cttcaggtca tgtcggcaac aatcttgtaa gagagcttaa
     3661 ccgtcgaaaa attaaaccga gagttgtaat tcacaacccc gaacatgcgg aaaagccgct
     3721 taaaggactc gattttgaag cagtcatggg agatgtaacc gaccttcagt caatgataga
     3781 ggcatttaag ggtgccgaaa tggtctatca ctgtgccgca tggatctcaa taacgccggg
     3841 gatgtacaaa aaactgagag ttgtcaatgt tgaaggcacc ttgaatgttc ttgaagcatg
     3901 cagaaggaat catgttaaaa aagttgttta tgtgagctct atcgaagcac tcggagatcc
     3961 gggagaaggt gttattgcaa aagaggagat gggatttaat ccggacaagg cgatgcttga
     4021 atacggtgtg acaaaagcgg aagcagctct tcttgttcag gaagcagtaa agaacggaat
     4081 ggacatcgta acagtatgcc ctgtcggaat aatcggtccg tatgacttca aatcttcaca
     4141 gatgggcaac atgatgatcg atttctacaa aggcaaactg ccggcatatc ccggatacgg
     4201 cggatttgac tgggtcgatg tcagagatgt cgccaatgcg ataattcttg ccggagagaa
     4261 aggaaaaaaa ggggaatcat atcttctcac aggcggtcac acaacaaact ttgaaatgat
     4321 ggaaatactt gaaaaagtga caggaaagaa aaaacctaag atcgctcttc cctactggct
     4381 tatgtcatcg gcaggatttt ttgccgaatt ttattataaa gcaaccggca aggaagcggt
     4441 gataacacgc gacagcgcaa agatcctcaa aagcgatctc cgtgcatcat cagaaaaagc
     4501 gaaaagggaa cttggatatg accccagacc tcttgagcag acattcaaag atcattttga
     4561 gtggttgatt gaaacttatg gcaaatgatc taatcatgct ccacaaacac tttcccatct
     4621 ttaaatgtaa cggaaaaaga accggattca tttagaaact tctttctctt tatcattctg
     4681 tcattctggg ttacaacagc aaaaccgcgt tccagcggcg aaagcggatt attggtctca
     4741 agttcttttt cgagagctga aagatcttta accgcatcga atataagttc tttaattttt
     4801 gaagccgctg ttaaaagcag tttttccgta cggtgacagg tctgactgat tatttcacct
     4861 ggattcttta gcgaaagagt catgttatca agaaacatca attttgaatt tattgagctt
     4921 tctaccgttt tcttcatatt cagaacagca ttggcggctt gatccagcaa aacggtgccg
     4981 tcaggaaata taagttccgc cgcggcagtc ggtgttgaaa ccgaataact tgccgcaaga
     5041 tcaagaatgg tggtgtctgt ttcgtgtcct accgcactta ttaccggctt tcggcatttt
     5101 atcacagccc ttgcgagctc ttctgaattg aaacagtcga gatcctcctt tgatccgcca
     5161 cctcttccga taactattgc atctatatca ccgatatctg acattctttc aagagctttg
     5221 atgagatcaa gatgagcatc tctgccctga actgaagaag gtgacagaat aatttgtgcc
     5281 ggagggaacc gttttcttga aacttttatg atgtcatgta tcgccgcacc gctttcactt
     5341 gtaactacgc ctatcgtatg gggaaactgc ggaagctgtt ttttatggct gacatcaaaa
     5401 atcccttcct ggctcaattt ttccttgagc attttaagtt tcaccgccgc cataccttca
     5461 ccatacggaa tgatgagatt tgcgatcaga gttatctccg atctctgctc ataaacagaa
     5521 agtttcccgt atacaatata ttctgaatca accttcagtt ctgcatgatt cattttctgc
     5581 tggtttttaa ataatgcaca tttgatctgg gcttcactat ccttgagtgt aaaatagata
     5641 tgtcccgaat aagctttgct taaattggtc aactctccgc ggactgcaac aaaaccgaaa
     5701 gctccttcca ctgcatgttt aagatctctg acaagagcgg aaaccggaac aacaacatcg
     5761 ttcatcatct gatcttcttc agagctttca ttttaagcgc attcgggata tttctgcttt
     5821 tggcaatatt tttcagatca tttatcctta ttacatcaag cagtgccatc gaagttttca
     5881 gaggtgtctt ggggttgttc accagcgcaa gctttacctg ataacttttt gaaagtgact
     5941 tcactgccgc aatataggca attatatcaa gtgcaacagc ggcatcggct gctattgaaa
     6001 ctatctcttt ttcggtgatc cttgcatttg ccagaaccga aatcgcgatc tgtcttctgg
     6061 ggtcctttat taatatcttc cttacctcca tgttgcccat catggcgagc tttaccttct
     6121 gacccattga catgtccttt atcatttcca tcatactttt tctctgaacg gtcatgtctt
     6181 cggcattcag accttcaaaa acttcatcgg aaacaaggaa atcgggaaaa tcaaaatctt
     6241 catcaccgag aacgacaaca tcttcatctt cctgtcccgg ctgttcatcc ggctttattt
     6301 cctcatccgg tttttcaccg ctctcaactt ctgccggaat atcttttacg acataaccga
     6361 cctgctcgat ataaagatca taaagaacag ggctgtatgt ggaaaaaaat ttaacaaaat
     6421 catccttctt cggcgctgta aattcgacaa aatccttgtg tgttataagc atcttccaca
     6481 gatcttcatc ctggatcata
//
LOCUS       DAOOYB010000161         6488 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_13646_length_6488_cov_9.903622, whole genome shotgun sequence.
ACCESSION   DAOOYB010000161 DAOOYB010000000
VERSION     DAOOYB010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6488
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13646_length_6488_cov_9.903622"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="PL056_09495"
     CDS             <1..509
                     /locus_tag="PL056_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47292.1"
                     /translation="GLGSDRVWKIDPATNARAADLPEGKTIGVGSHYTYSDFTGSTAF
                     NFTAPRGVWNYRFGKPSACSIPSSIFVKAYVPDKTYIGVRLRSLDKNGNPVTGWIPEI
                     VTAEKYFDIPKGMGETEIDLADYREYFLTAAKYEIEVLMTTTDKSVLPILNNANVMWE
                     YDSDACAN"
     gene            complement(510..1400)
                     /locus_tag="PL056_09500"
     CDS             complement(510..1400)
                     /locus_tag="PL056_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006964123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HPM47293.1"
                     /translation="MTEESKFVCTNEQEVKRVTFIGLVLNLLLSALKITAGIFGKSQA
                     VIADGVHSISDCATDIAVIVGSTYWSAPPDDSHPHGHRRIETAITVFIGIALFAVALL
                     MGWNAVTTFTDEDDPPRLIAFFASIISIIVKELLYHWTVRKGKKIKSSAVVANAWHHR
                     SDAISSIPASLAVIFSIFMPDHSFIDNIGAIAVCLFILHAAYRISWPALKELTDAGAG
                     EQICVSIENIARSVEGVKDVHKCRTRYLGSGIQVDMHVLVNPEMSVRSGHEISAAVKK
                     ELLDQVDDIIDVVVHLEPFE"
     gene            complement(1410..2555)
                     /gene="gmd"
                     /locus_tag="PL056_09505"
     CDS             complement(1410..2555)
                     /gene="gmd"
                     /locus_tag="PL056_09505"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011879425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HPM47294.1"
                     /translation="MAKKALITGITGQDGSYLAELLLSKGYEVHGIKRRSSMFNTDRI
                     DHLYQDLHENNVRLKLHYGDLTDATNLIRIMQETQPDEIYNLAAQSHVKVSFETAEYT
                     GNADALGTLRLLEGIRILKLEKKTKFYQASTSELYGKASEVPQNESTPFYPRSPYGAA
                     KLYGYWITVNYREAYGIHASNGILFNHESPRRGETFVTRKITRAVARIVLGLQSMLYL
                     GNLDAKRDWGHAKDYVKAMWMVLQQEKGDDFVIATGRTMTIRDFVKLSFETVGIEIAF
                     EGKGVDETGIVKSLDIQKVLETTGKSYIELTPGMTVLKIDRNYFRPTEVDILQGDASK
                     AKEILGWQAETTVEEMAVEMVMSDLHEAKKEQLLIKNDYFVRNNYGE"
     gene            2720..4705
                     /locus_tag="PL056_09510"
     CDS             2720..4705
                     /locus_tag="PL056_09510"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HPM47295.1"
                     /translation="MDGNNFCFGCFSPKGSDYKCKNCGYVYDLKEGPEQSHLFPGTTL
                     NNRYILGKVIGAGGFGITYIAFDPKLQVRLAIKEFFPRQIASRDKETRNVVPFGSSEK
                     NDFINGLKYFLNEARSAAMFETHQNIVGVKDMFEENGTAYMVMPYISGTPFDSFLTMR
                     GGVVDFATAMSVLAPIMDAVAAIHEKNLLHRDISPENIFITSEGVPKLLDFGSARPTV
                     ASETLSMTPVIKEGYAPLEQYSRQGEQGPWTDIYSMAATIYKAVSGQTPPPAMERFTN
                     DALIELSKVCPDIPGYAEKAIVKALSLRASDRFQNMLEFLNALTKGKSVEPLKFNETS
                     SKKPGNKLVLIAGSAVAGIAVISVGLFFALSSPKKVEPVEKPVEKVEKVEKPVEKPVE
                     NLSDDLSDKVSTKSEVLTKSEVEKVEKVDKVEKPVEKKKNIAKHKEKIEKKPEIIEER
                     PVEKKKDVGVSIGGSGNFVIPREVEKGLTPEQYFKDGFKYELARNMAQAISYYEEACK
                     GKFTNGCYRLGKVKKDDDILSKARGMYEKECKKGTSSSCITLAEMMLKGEGGAASPFE
                     ATTALAKECSAGKVNGCFLLAGIWEKGENINKDLEKARKYYGYSCDQGSKDGCFKYAE
                     MLENGIGGPADAQKAAENYKKACQKGFVRACMKAKKQ"
     gene            complement(4708..4941)
                     /locus_tag="PL056_09515"
     CDS             complement(4708..4941)
                     /locus_tag="PL056_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47296.1"
                     /translation="MNEQNRSYDEIQSKTVILEDNEGVNDMLSALNTGRAALDKKKKE
                     MELAERNKKIAIFIAVFVVAAAVLAGIFMVMGR"
     gene            complement(4954..5838)
                     /locus_tag="PL056_09520"
     CDS             complement(4954..5838)
                     /locus_tag="PL056_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008682367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HPM47297.1"
                     /translation="MDIKKRDRFINVVGQLCCPGSYGPVVNLHSKGAPMPSRDDLERT
                     MELLRAIVFPGFYMESDINAANISFYTGAKLDKVFSIMKEQIKRGLCFECQGTSGREC
                     VDCDTIAEEKAFEFIEDLPEIRRMLALDVEAAYEGDPAAINQGETIFCYPGVRMLTSY
                     RIAHSLFKIGVPLIPRIITEMAHSETGIDIHPEATIGERFFMDHGTGIVIGGTSIIGR
                     NVKIYQGVTLGAKSFPKDAQGNPVKGVPRHPIVEDDVVIYSNATILGRITIGKGAVIG
                     GNTWVMENVPAGARILAK"
     gene            complement(6052..6435)
                     /locus_tag="PL056_09525"
                     /pseudo
     CDS             complement(6052..6435)
                     /locus_tag="PL056_09525"
                     /inference="COORDINATES: protein motif:HMM:NF036995.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 N-terminal domain-containing protein"
BASE COUNT         1904 a         1428 c         1309 g         1847 t
ORIGIN      
        1 tgggacttgg aagtgaccga gtctggaaaa tcgatccggc aactaacgca agagcggcag
       61 atcttccgga aggaaaaacc atcggagtcg gatctcacta tacctacagt gattttaccg
      121 gctctactgc tttcaacttc acagcaccca gaggtgtctg gaattacaga ttcggcaaac
      181 cttcagcatg ctccatccct tcatcaatct tcgtaaaggc atatgttcct gataaaactt
      241 acattggtgt ccgtctaaga tcgctggaca agaacggtaa tcctgtaaca gggtggatac
      301 ctgagatagt aactgctgaa aaatatttcg atattccgaa aggtatgggt gaaacagaga
      361 tagatcttgc tgattaccgt gaatattttc tgacagcggc aaaatacgaa attgaagttc
      421 ttatgacaac tacagataag agtgttcttc cgatacttaa caatgcaaat gtaatgtggg
      481 aatacgattc tgacgcctgc gcaaactgat cattcaaatg gctccaaatg aacaacaaca
      541 tcaattatat catcaacttg atccaataac tcttttttga cggcagcact gatctcatgg
      601 ccacttctga cgctcatttc cggattgaca agaacatgca tatcgacttg aataccgctt
      661 ccaagatacc ttgttctgca cttgtggaca tctttgactc cttcaacact tcttgcgata
      721 ttttctattg aaacacatat ctgttcaccg gcaccggcat cggtaagttc tttaagtgcg
      781 ggccaggaaa ttctgtatgc ggcatgcaga ataaaaagac agaccgcaat cgctcctata
      841 ttgtcaataa agctgtgatc aggcataaag attgagaaaa ttaccgctaa cgatgcagga
      901 atggaactga tcgcatctga acggtgatgc catgcatttg cgacaacggc cgacgacttt
      961 atctttttcc ccttgcgcac agtccaatga tacagaagtt ctttcacaat tatggaaatt
     1021 atcgaggcaa aaaaagcgat caaccgtggt ggatcatcct cgtccgtaaa agttgtcact
     1081 gcattccagc ccattaaaag agcaactgca aaaagagcga tccctataaa aacagttatt
     1141 gctgtttcga tccttctgtg accgtgagga tgagaatcat ccggaggagc actccagtag
     1201 gtagagccga caataaccgc aatatctgta gcacagtcgc ttatgctgtg aactccgtca
     1261 gcaatgaccg cctgactttt tccgaaaata ccggcagtta tctttaaagc agaaagcaga
     1321 agatttaaaa caagcccgat aaatgtgact cgtttaactt cctgctcatt ggtgcagaca
     1381 aattttgatt cttcagtcat tggggaaagc tactcaccgt aattgtttct gacaaagtaa
     1441 tcgtttttta taagaagttg ctcttttttt gcttcatgta aatctgacat gaccatttca
     1501 acagccattt cctcaactgt ggtctctgcc tgccaaccaa gaatttcctt cgcttttgat
     1561 gcatcaccct gaagaatgtc aacttcagtc ggcctgaaat agtttctgtc aattttaagc
     1621 actgtcatgc ctggcgtcag ctcaatatat gatttgccag tagtttcaag caccttctga
     1681 atatcaagcg atttaacgat acctgtttca tcaacacctt taccttcaaa agcgatttct
     1741 atgccgactg tttcaaatga cagttttaca aaatccctga tcgtcattgt tctgcctgtg
     1801 gcaattacaa aatcatctcc tttttcctgc tggagcacca tccacattgc tttcacatag
     1861 tcttttgcat gtccccagtc cctttttgca tcaagattcc caagataaag catgctctgc
     1921 agtcccagaa caattctcgc aactgccctt gttatttttc ttgtgacaaa agtttcgcct
     1981 cttcttgggg attcgtgatt aaaaagaatg ccgttacttg catgaattcc gtatgcctca
     2041 cgatagttta cagttatcca gtaaccatag agctttgccg ctccatatgg gcttctggga
     2101 tagaacggag ttgattcgtt ctgaggaacc tcactcgcct ttccgtaaag ctcagaagtt
     2161 gatgcctgat aaaattttgt tttcttttca agtttaagaa tccgtatccc ctcaagaagc
     2221 cgcaaagtgc ccagagcatc agcatttcct gtatattccg ctgtttcaaa cgaaactttt
     2281 acatgggact gagccgcaag attatatatc tcatcgggtt gggtttcctg catgatacgg
     2341 atcagatttg tcgcatcggt cagatcaccg taatgaagct tgagccgcac attattttca
     2401 tgaaggtcct gataaagatg gtcaattcta tccgtgttga acatcgaact tcttcttttt
     2461 ataccgtgaa cttcataccc cttgcttaaa agaagttccg caagatatga accgtcctga
     2521 cccgtgattc ctgtgattag tgcttttttt gccatgttgt ctctcctcaa attactcact
     2581 aaagtataga aagagataaa acaaagtcaa aaacctgtaa aaaccggtat aacttggaaa
     2641 aatcttcaga aagaaaaaaa attgaactat ttcaatataa agtgtatgtt ttgaaatctc
     2701 ggttttttag gagagtttca tggatggcaa caacttctgt ttcggatgtt tcagccctaa
     2761 gggtagcgac tacaaatgca aaaactgcgg atatgtctat gatctgaagg aaggtcctga
     2821 gcagtcgcac ctcttcccgg ggactacact taacaaccgg tatatcctcg gaaaagtcat
     2881 cggtgcgggc ggtttcggga taacatacat cgcatttgac ccgaaattgc aggtccggct
     2941 cgcgatcaag gaattcttcc caagacagat cgcatcaagg gacaaggaaa ccagaaatgt
     3001 agttcctttc ggcagcagtg aaaaaaatga tttcataaac ggtcttaaat atttccttaa
     3061 tgaagcacga agcgccgcaa tgtttgaaac tcaccagaac attgtcggag tcaaggatat
     3121 gtttgaagag aacggcacag cttacatggt catgccgtac atcagcggaa ctccttttga
     3181 ttcattcctc acaatgagag gcggtgtcgt cgattttgca acagccatgt cagtccttgc
     3241 acccatcatg gatgccgttg ccgcaataca cgaaaagaac cttcttcaca gagatataag
     3301 ccctgaaaac atttttataa ccagcgaagg tgttccgaag cttctcgatt ttggatctgc
     3361 aaggccgaca gtcgcaagcg aaactttgag catgacacct gtaataaaag agggctatgc
     3421 accccttgag cagtacagca gacagggtga acagggtccg tggacagata tctattcgat
     3481 ggcggcaaca atttataaag cggtcagcgg tcagacccct cctccggcga tggaaagatt
     3541 tacaaacgat gcattgatcg aactttcaaa agtttgtccc gatattccag gttacgctga
     3601 aaaagctata gtaaaagccc tttcgctccg tgcttcagac cgttttcaga atatgcttga
     3661 atttctgaat gctctcacaa aaggaaaaag tgtggaaccg ctgaagttca acgaaacatc
     3721 gtcaaaaaaa ccgggaaata aactcgttct gatagccggc tcagcagttg caggtattgc
     3781 tgttatttcg gtcggacttt tctttgcact ctcttcaccg aaaaaggttg agcccgttga
     3841 gaagcccgtt gagaaggttg agaaggttga gaagcccgtt gagaaaccgg ttgagaactt
     3901 gtccgacgat ttatccgaca aagtttcaac gaagtcggaa gttttaacaa agtcggaagt
     3961 tgagaaggtt gagaaagttg ataaggttga gaaaccggtt gaaaaaaaga aaaatattgc
     4021 aaaacataaa gagaaaatag agaaaaaacc cgaaataatt gaagaaagac cagtagaaaa
     4081 gaaaaaagat gttggtgtct ctatcggagg aagtggaaat tttgtcatcc ccagagaagt
     4141 ggaaaaaggg ctgacacccg agcagtattt caaggatgga tttaagtatg aactcgcaag
     4201 gaacatggct caggcaattt cctattatga agaggcgtgc aaaggaaaat tcaccaacgg
     4261 atgctatcgg ctcggaaaag ttaaaaaaga tgacgacatc ctttcaaaag caaggggaat
     4321 gtacgagaaa gagtgcaaaa aagggacttc cagcagttgc ataacccttg cagaaatgat
     4381 gctcaaaggt gaaggtggag cggcaagtcc ttttgaagcg acaactgctc ttgcaaaaga
     4441 gtgttctgcc ggaaaagtca acggctgttt tctccttgca ggtatttggg aaaaagggga
     4501 gaacataaac aaagatctcg aaaaagcccg taaatattat ggatattcat gtgatcaggg
     4561 gtcaaaggac ggttgtttca aatatgctga aatgctcgag aacgggatag gcgggccggc
     4621 agatgcgcag aaagctgccg aaaattataa gaaagcttgt cagaaaggtt ttgtcagggc
     4681 ctgcatgaaa gcaaagaaac agtagaatta tcttcccatt accataaaaa ttccggcaag
     4741 aactgccgca gcaacaacaa aaacagcaat gaaaatggct atttttttgt tcctttcagc
     4801 cagctccatc tcttttttct ttttatcaag tgccgctctt cctgtgttga gggcgctcag
     4861 catgtcgttc acaccttcat tgtcttcgag aataaccgtc ttactctgaa tttcatcata
     4921 acttctgttc tgctcgttca tttaaacccc cttctatttt gcgagaattc ttgctcctgc
     4981 aggaacattt tccataaccc atgtatttcc accgataacc gccccttttc ctatagttat
     5041 ccttccaagt attgtggcat ttgaatatat cacgacatca tcttcaacga tcggatgcct
     5101 tggaacacct ttgacggggt tgccctgagc atctttcgga aaactttttg ctccaagagt
     5161 aactccctga tatatcttga catttctgcc aattatgctt gttccgccga taacgattcc
     5221 agtcccgtga tccataaaga acctttcccc gatagtagct tcaggatgga tgtcaattcc
     5281 agtctctgaa tgtgccattt ctgtaattat tctcgggatc agaggaaccc ctatcttaaa
     5341 aagcgagtgg gctatccgat aacttgtcag catccttaca ccgggatagc agaagatcgt
     5401 ctcaccctga tttattgcgg caggatcacc ttcataagcc gcttcaacat caagagccag
     5461 cattcttctt atttcgggaa gatcctcaat aaattcaaaa gctttctctt cagctattgt
     5521 gtcacagtcg acacattccc ttccgcttgt gccctgacat tcaaaacaaa gtcccctttt
     5581 tatctgttct ttcattattg aaaaaacctt gtcgagcttt gctccggtat aaaagctgat
     5641 atttgcggca tttatatcag attccatgta aaacccggga aaaacgatag cccggagtag
     5701 ttccattgtc ctttcaagat catccctgct cggcatcggc gccccttttg aatgaaggtt
     5761 caccaccgga ccgtatgaac ccggacagca gagttgaccg acaacattta tgaacctgtc
     5821 tctttttttg atatccattg ttccctccga ataaaaacaa gtcattatga caattttgaa
     5881 ggatgtgtca agttcaggag cagtacgcgt taaaacgaag taaaaatatc cgctttagga
     5941 ggttaaccgg gacacaaata aacttgaaga ccaaatcaga caaattggtc taatttaatc
     6001 acgccgtttc agaacaggct gtttaggagt agatcttatt ccgtcgcttt tttaatgcat
     6061 atttcttata aatccgagat catattcagc tttaaaaatc tccagagcat tcgagattga
     6121 ctgtcttttc ctggcaggca ggttttcatt gacagttttt gtctgataaa agctcctttc
     6181 tacatgattt tcaattctta taagttcaac ataatgattc cagcttaatt gtcgtgacag
     6241 tgtaacgaca ttttcatata atctgaaaaa cttaacgatt ttatacagtt ccttttggtc
     6301 aatgtttaag tcagctgcaa tgtttttaat taaaaactgt ccataatcag ctctttcctt
     6361 gttttcaatt tcatctctca cgattctttc accgatctgc cagtatgtct gaactttgat
     6421 gttgtcaact gctttgtaag ctcttgcaag acctttgctt aatttatatc gtcacttgat
     6481 taattttg
//
LOCUS       DAOOYB010000162         6482 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_13671_length_6482_cov_4.546911, whole genome shotgun sequence.
ACCESSION   DAOOYB010000162 DAOOYB010000000
VERSION     DAOOYB010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6482
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13671_length_6482_cov_4.546911"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="PL056_09530"
     CDS             <1..486
                     /locus_tag="PL056_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47298.1"
                     /translation="GVFRSGVKGVGIGADDRAGCAIVWLLKDLGHSLLIFDGEEDHQL
                     GSRYLLKHHPGIMNEINNDHQFAVSFDRKNGREFKCYDVGSDEFRRYIMEKTGYREPD
                     RSSHTDIVVICEKICGVNLSVGYYNPHYENESLNVNEWLNTLNIARNWFSEPGLPRFV
                     R"
     gene            complement(556..1194)
                     /locus_tag="PL056_09535"
     CDS             complement(556..1194)
                     /locus_tag="PL056_09535"
                     /inference="COORDINATES: protein motif:HMM:NF024790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HPM47299.1"
                     /translation="MNNIIKYILLFIVLFITGLMIYYFKRFEYVFKLNETLYISAAVF
                     TAGVFINRKFSERLLFWKTLNHELTHIFFSLIFGNKITGLHATEEGGGLARYQGRSNF
                     VITLAPYFFPLVPFVLILFSIIIRPEIAHYFRFIIIFVYALSLCSQIEDFRYEQSDIK
                     EEGKIFSTLFIVVMNVQSLLFFAAYGNNRTDSYQHTIMTLFSELIHYVKVIF"
     gene            complement(1275..4196)
                     /locus_tag="PL056_09540"
     CDS             complement(1275..4196)
                     /locus_tag="PL056_09540"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPM47300.1"
                     /translation="MKVLKISGKNLNSLKGVWSIDFTASEYVSDGIFSITGATGAGKS
                     TILDALALALFGRTPRLKNISKSTNEIMSRQTGECFAEAEIETSKGRFRFSWNQAKAR
                     NRADGNLQSQKREVAQLDSGKILADTINTADNIVIELTGLTYEQFSRSILLAQGDFAA
                     FLKAGENERSEILEQITGTKIYSEISIKAHEKQKEESEKLKLLEAEIKGIQILKDEDV
                     AALKGDLKVKEEENVAMKVKAENYSKVKTLLENIKNNRTELDEINKLLPQKKKALENN
                     SKLKDEKLLTLKSAKDDQETQQILFKNVRSLDQSISEKNKNIKGLEKDSNSKEKLLKT
                     KNDEHKKEKEKKEKLIVKIDTLKKYFEMNDTDEKLVSELSGLKEKVEKFFEIVKNIDD
                     ISKSLTSFTKDLEKKKNEISNSEKEHEKTKKAEGLIKTEIEELNKQSKELLKGETLGS
                     YRKIKEGLLREVKLIEKILSHEEERKKLQNGAPCPLCGSKDHPFAEGNVPQKDETEQA
                     IEKLEKLISKVEDIELKLNNKRELSQKAVLDIKTAESNISGLKKDKTSIESKISDINK
                     NIAKLSSDSERIEDELSSHFEQLKIADSSRISFGDNLATLEKRKNEWVSNLKENDVVK
                     NTLSEIDLKLSGLSSEIKSMEEAIAELKKKVREEKTVLEDLNKKRSDLFGTRTVDEEE
                     NKLKAAVNKLEEDLKKVSDIVVKATDEFNKDITKQELLLKITDESQTELEKIKLALPP
                     ETYPGNDSIEELSEKLEYIRVELEKNGELIGATKEKLKSNEKARKESALKQEKIEIQR
                     KELEKWHLLDKLIGSADGKKFRTFAQGLTFEIMVRHANAKLLEMTDRYFLKRDGESPL
                     ELNIIDNYKAGDIRSTKNLSGGESFIVSLALALGLSSLSSNNIRIDSLFLDEGFGTLD
                     EETLETALNILAGLHKDGKLIGVISHVSAMKERIATHISVVKVRDGNSYIEGPGVEFN
                     P"
     gene            complement(4207..5436)
                     /gene="sbcD"
                     /locus_tag="PL056_09545"
     CDS             complement(4207..5436)
                     /gene="sbcD"
                     /locus_tag="PL056_09545"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011784111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HPM47301.1"
                     /translation="MKILHTSDWHLGKTLFGEKRYDEHEKFLTWLIGVLKDENIDLII
                     IAGDIFDTTTPSNRAQELYYSFLCNTKTTSCRHVVIIGGNHDSPSFLSAPQELLKHLN
                     IHVVSDISDRENEIIEIKNNEGKQELIVCAVPYLKDRDVRLSVPGESGTDKEANLLDG
                     IRSHYEEIHAKAKDLKEKYNVPLISTGHLFTKNGKVEKDDGVRELYIGSLARVDGTIF
                     PDFVDYTALGHLHVPQTVGERDNVRYSGSPIPMGFNEGYQNKKVIIVDIRDFSTTITE
                     KTVPVFRKLVSLKGNEKEVLEQFESIKEDTAEELEVWVELIGKGFETTAKIMEIAGKI
                     EESGHGKVLKKENADKRMTVISPLEEFEALEELGPVDVFERLMEVKEVSEDNKKELKG
                     AFEALIYEIQNEDKNKE"
     gene            5629..>6482
                     /locus_tag="PL056_09550"
     CDS             5629..>6482
                     /locus_tag="PL056_09550"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HPM47302.1"
                     /translation="MDLKSRYRGSILGMASGDAMGSAFEFISRDEVPEIIEDMAGGGC
                     FDWKPGQWTDDTAMAVCLGASLINCRGFDLKDQIERYCQWYQNSFDIRTALGLEKDGN
                     GSGSIIPCRDIGNTTREALDNYLYSGDPESGSSDPMTSGNGSIMRLAPVPLFYRKDPA
                     MAVEFGGRSSLTTHGSAECIDACRLLSAVIVKALKGLSKKDILAIDPSIFSSPKIAMT
                     AKGGYQIKTMDQIKGSGYVAESLEAALWCFYTTDTFEKAIIAAVRLGDDTDTTAAVTG
                     QIAGAYYGA"
BASE COUNT         1766 a         1311 c         1166 g         2239 t
ORIGIN      
        1 ggtgtgttcc gttcgggggt aaaaggcgtt ggtatcggtg ctgatgaccg tgcgggttgt
       61 gcaattgtat ggcttcttaa ggatcttgga cattctcttc ttatttttga cggagaggag
      121 gatcatcagc ttggatcaag gtatctgctc aaacaccatc ccggaataat gaatgaaata
      181 aacaatgatc atcagtttgc agtttcgttt gacaggaaaa acggcaggga atttaaatgt
      241 tatgatgtcg gcagtgatga attcaggcgt tacattatgg aaaagacagg ttatcgtgag
      301 ccggacagaa gttctcacac agacattgtc gtaatatgcg aaaagatctg cggagtcaat
      361 ctgagcgttg gttattacaa cccgcactat gaaaacgaat ctctcaatgt taacgaatgg
      421 ttgaacacgc ttaacattgc aaggaactgg tttagtgaac cgggcctgcc aagatttgta
      481 agataatttc tttggaagta agttatgaag aaaactatga ttcttgtttt gtttgcggtt
      541 ttttgttcat aaattctaaa atataacctt tacataatga ataagttctg aaaacagtgt
      601 cattatcgta tgctggtaag aatcagttct attgttgccg tatgcagcaa aaaacaataa
      661 cgactggaca ttcattacaa caatgaacag tgttgaaaat atcttgccct cttccttaat
      721 atcgctttgt tcataacgga agtcttcgat ctgactgcaa agcgaaagag cataaacaaa
      781 aataatgata aatctgaaat aatgtgctat ctcaggccgg ataataattg aaaaaagaat
      841 aagcacaaac ggaaccaacg gaaaaaaata cggagcgagc gttattacaa aattagatct
      901 accctgatat ctggcaagac ctccaccttc ttcagtcgca tgaagacctg ttatcttatt
      961 tccgaaaatc agtgaaaaaa atatgtgtgt cagttcatga ttgagcgttt tccagaaaag
     1021 cagtctttcg ctgaacttgc gatttataaa aacacctgcc gtaaacaccg ctgctgaaat
     1081 ataaagagtc tcgttaagtt taaagacata ttcaaatcgc ttgaagtaat aaatcatcag
     1141 cccggttata aaaagaacga taaacagcaa tatatacttg ataatgttat tcattttacc
     1201 tcaaaaatgg ctttcaaaac tctggttcag aacagaactg gaatgtcttt cagataagtt
     1261 gtttgaattg tccattaagg attaaactcc acaccgggac cttcaatgta actgtttcca
     1321 tctcttactt tgacaacaga aatgtgagtt gcaattcttt ctttcatggc tgagacatgg
     1381 gatatgactc caataagttt gccgtcttta tgcagtccgg caagaatgtt caatgcggtt
     1441 tcaagcgttt cttcatcaag tgtaccaaac ccttcatcaa ggaaaagact gtctattctt
     1501 atgttattac ttgaaagact ggataatcca agcgcaagtg caagactcac aataaagctt
     1561 tctccacctg aaagattttt ggtggaacga atgtcaccgg ccttgtaatt gtcaatgatg
     1621 ttaagttcca acggactttc cccgtctctt tttaaaaaat agcggtcagt catttcaaga
     1681 agttttgcat tagcatgtct taccattatt tcaaaagtga gtccctgtgc aaatgtacgg
     1741 aattttttac cgtcagcaga gccgatcaat ttgtcgagca gatgccattt ttcaagttcc
     1801 tttctctgga tttctatttt ttcctgtttt aaagctgatt cttttctcgc tttttcattt
     1861 gatttaagct tttcttttgt tgctcctatc aattcaccgt ttttttccag ttcgaccctt
     1921 atatattcca gtttttccga aagctcttcg atggaatcgt tgcccgggta tgtttcaggg
     1981 ggcagagcca gttttatttt ttcaagctcc gtctggcttt cgtctgttat ttttaaaaga
     2041 agctcctgtt tagtaatatc cttgttgaat tcatcagtcg ctttgacaac tatatcagac
     2101 acttttttga gatcttcttc aagtttgttg actgctgctt tcagcttgtt ttcctcttca
     2161 tcaacagttc ttgtcccgaa aagatcgctc ctctttttat tgagatcttc caaaaccgtt
     2221 ttttcttctc taaccttctt tttaagttca gctattgctt cttccattga cttgatctct
     2281 gaagaaagtc cgctcagttt taaatcaatt tctgaaagag tgttttttac cacatcattt
     2341 tctttaagat ttgacaccca ttcatttttt cttttctcaa gtgtcgccaa gttatctcca
     2401 aaacttattc ttgatgaatc agctattttc aactgttcaa aatgacttga caattcatct
     2461 tcaatccttt cagaatcaga agaaagcttt gctatatttt tatttatgtc gctgatcttt
     2521 gattcaatgg atgttttatc tttttttagc cctgaaatgt ttgattcagc ggtttttatg
     2581 tccaaaactg ctttctgcga gagttctctc ttattattca gttttaactc tatgtcctcg
     2641 actttcgaga taagtttttc taatttttcg attgcttgct ctgtttcatc cttttgagga
     2701 acattacctt cagcaaatgg atgatctttt gatccacaaa gaggacaagg agcaccgttt
     2761 tgaagcttct ttctctcttc ttcatgtgac aaaatctttt ctatgagctt aacttctctt
     2821 aacagccctt cctttatttt cctgtatgaa ccaagggttt cacctttaag aagttcttta
     2881 gactgcttgt taagctcttc gatttcagtt tttatcaaac cttcagcttt tttcgttttt
     2941 tcgtgttctt tttcactatt tgaaatctca ttcttctttt tttccagatc ttttgtaaaa
     3001 cttgtcaggg atttgctgat atcatcaatg tttttaacga tctcaaagaa cttctccact
     3061 ttttctttca gtccgcttaa ttcagacaca agcttttcat cagtatcgtt catctcaaaa
     3121 tattttttca atgtgtcaat ttttacaatc agtttctctt ttttttcttt ttctttttta
     3181 tgttcatcat tttttgtttt cagcagtttt tcttttgagt tagagtcttt ttccaatcct
     3241 ttgatgtttt tgtttttttc agaaatcgac tggtcaagag atctgacatt tttaaaaaga
     3301 atttgctgtg tttcctgatc atctttagcc gatttaagag ttaacagctt ttcatcttta
     3361 agtttgctgt tattttcaag agctttcttt ttttgaggaa gcaatttgtt gatctcatct
     3421 agttcagttc tgttgttttt aatgttttca agaagagttt taacttttga ataattctct
     3481 gctttcactt tcatggcgac attctcttct tcttttacct tgagatctcc ttttaacgcc
     3541 gctacatctt cgtctttaag aatttgaata cctttaattt ccgcttcaag aagtttcagt
     3601 ttttcagatt cctctttttg tttttcatgg gctttgattg agatctcaga ataaatcttc
     3661 gtacctgtga tctgttcaag gatctctgac cgttcatttt caccggcttt taaaaaagcg
     3721 gcgaaatcac cctgggctag aagaatcgaa cgggaaaact gctcataagt tagtcctgtc
     3781 agttcaataa caatattatc agctgtattg atcgtatcag ccaggatctt tccggaatca
     3841 agttgggcaa cttcacgctt ttgactctga agattgccgt cagccctgtt tcttgcttta
     3901 gcttgattcc agctaaaacg gaagcgccct tttgatgttt ctatttcagc ttcagcaaaa
     3961 cattcccctg tttgacggga cattatttca tttgttgatt tgctgatatt ttttaatctc
     4021 ggagttctgc caaaaagtgc caaagccaaa gcatcaagga tcgttgattt tcctgcccca
     4081 gttgcaccgg ttattgaaaa gatgccgtct gacacatatt ccgaagcagt aaaatctatg
     4141 ctccagacac cttttagcga attcaggttc tttcctgaaa ttttaagcac tttcatgttt
     4201 tacctcttat tctttattct tatcttcatt ttgaatttca taaatcagtg cttcaaatgc
     4261 acctttcagt tccttcttgt tatcttcact cacttctttt acttccatca acctttcaaa
     4321 aacatcaacc ggtccaagct cctcaagtgc ttcaaattct tcaagcggag atattactgt
     4381 cattctttta tcagcattct cttttttaag aaccttgcca tgcccgcttt cttcaatttt
     4441 tccagctatt tccataattt ttgcggttgt ctcaaaccct ttcccaataa gttccaccca
     4501 cacttcaagt tcttctgcgg tgtcttcttt tatgctttca aactgctcaa gcacctcttt
     4561 ttcgtttccc tttagcgaaa caagctttct aaataccgga acagtttttt cagttatcgt
     4621 tgttgaaaaa tctctaatgt ccacaattat aaccttcttg ttttgatatc cttcattaaa
     4681 acccatcgga atgggagaac cactgtatct gacattatcc ctttccccga cagtctgcgg
     4741 aacatgaagg tgcccaagcg ctgtgtaatc aacaaaatcg gggaatatcg tcccgtcaac
     4801 cctagccagt gaaccaatat aaagctctct caccccgtca tctttttcaa ccttcccgtt
     4861 tttggtaaaa aggtgtcctg tcgaaataag aggaacatta tatttttctt taagatcttt
     4921 tgcttttgca tggatctctt catagtggct acgaatcccg tcgagcaaat ttgcttcttt
     4981 atccgtaccg ctttctccgg gaacagataa acggacatcc ctgtctttga gataaggaac
     5041 agcacaaact ataagctctt gttttccttc attgtttttt atttctataa tttcattttc
     5101 cctgtcggag atatcggaca caacatgaat attgagatgt ttcagaagtt cctgcggtgc
     5161 tgataaaaat gaaggggaat cgtggttccc accgattatc acaacatggc gacaggaagt
     5221 tgtctttgtg ttgcacagaa atgaatagta gagctcctgt gctctattgg aaggcgttgt
     5281 cgtatcaaaa atatcacctg caatgatgat caggtcaata ttttcatctt tgagaacacc
     5341 tataagccag gtgaggaatt tctcatgttc gtcatatctt ttctcgccga aaagggtttt
     5401 gcctaaatgc caatctgatg tgtgaagaat tttcatagct tacctcgttt gataatatta
     5461 ccgaccagta tttattacat taacaagtag aaaaaacaac ataacgatcc cctccaaaaa
     5521 aagtgggcag ttgttaaggt gaataatact caaccattac gacctatgtt gtcgggttgc
     5581 aaaaaataaa tatactacaa actgaagaaa agaaaagagg ggggtaacat ggatctcaag
     5641 tcaaggtatc gcggttctat tctcggtatg gcatctgggg atgcgatggg atctgctttt
     5701 gaattcatat caagagatga agtccctgaa ataattgaag atatggccgg gggtggatgt
     5761 tttgactgga aacccggtca atggactgat gacactgcca tggcagtgtg tctgggagca
     5821 agtctgataa actgcagagg ttttgatctt aaagatcaga tagagcggta ttgtcagtgg
     5881 tatcaaaata gttttgatat cagaactgca ctcggactgg aaaaagacgg aaatggatcg
     5941 ggcagcataa ttccctgcag agatatagga aataccacaa gagaagctct tgacaactat
     6001 ctttacagcg gtgatcctga gtcagggtca agtgatccca tgacttcagg gaacggttcc
     6061 attatgcggc ttgcacctgt tcctttgttt taccgcaaag atccggctat ggcagttgaa
     6121 tttggtggca gaagctctct tactactcat ggaagtgctg aatgtattga tgcctgccgg
     6181 ctgttatccg cagtaattgt taaagctctc aaaggtttat ccaaaaaaga tatacttgca
     6241 atagatccat ccatattcag ttcacccaaa atagccatga cagccaaagg cggatatcaa
     6301 attaaaacaa tggatcagat aaagggaagc ggttatgtcg cagaatcact cgaagccgca
     6361 ctctggtgct tttatacaac agataccttt gaaaaagcga tcatagcagc cgtccggctt
     6421 ggcgatgaca cagataccac cgcagctgta acaggacaga tagccggagc atattatgga
     6481 gc
//
LOCUS       DAOOYB010000163         6462 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_13743_length_6462_cov_7.350710, whole genome shotgun sequence.
ACCESSION   DAOOYB010000163 DAOOYB010000000
VERSION     DAOOYB010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6462
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13743_length_6462_cov_7.350710"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(156..569)
                     /locus_tag="PL056_09555"
     CDS             complement(156..569)
                     /locus_tag="PL056_09555"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HPM47303.1"
                     /translation="MVNNKKYYIHPALILGIIEQESSFNPQAKSHAGAYGLTQIMPQT
                     AKLLKCDYNKLADPELAINCSVKFLAALLTYNKGDLIKSLSGYNGGTHSTETKATTAD
                     GLLAGRIYNNPETKNYVISVLKRFEKFKTLICNKK"
     gene            801..1349
                     /locus_tag="PL056_09560"
     CDS             801..1349
                     /locus_tag="PL056_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HPM47304.1"
                     /translation="MKYVQPIRDMDLIEKLKVILKKQNYRNYFLFIFGINSGLRISDI
                     LRLKVSDVKNKTHVTIIEKKTGKEKRFKLNTSLQAEIKEYISNMSNDEYLFTPVNGKS
                     GKPISREMAWIILNKAAGELGIEEIGTHTLRKTFGYHFYQKTKDVALLQNIFNHSAPS
                     VTLRYIGINQDIIDSAIDEFSL"
     gene            1372..4464
                     /locus_tag="PL056_09565"
     CDS             1372..4464
                     /locus_tag="PL056_09565"
                     /inference="COORDINATES: protein motif:HMM:NF033527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HPM47305.1"
                     /translation="MERDNRLKVLNENEYNELYGHPGFSIEERSVFFSLSDSEVEERD
                     KLRSVSAKIFFILQTGYFKAKRIFFTLDTENSKDDLLFIIDRYYPGFSMDNLSVTDRV
                     RLFQQKIIADIFGYRIFKAPERKLFAERAAYLAKIHSNPAYIFNELKTYLDNEKIILP
                     MYSFFQDIIGKAISDEIKRCGEYIIKKMDKSLKDKMEDFLSAEDNSLYNLTLIKKDPA
                     NFKNSEIMQEVKKRQSLKPLYSFADKVLKELEMSSESIKYYSSLVSYYSVYRLKELKS
                     LATKYTYILCFIYNRFQQCNDNLINSFIYHVNDYFSDADKYAVQAIFEQKMTGTRNLK
                     KAGKILELFLDDNISGDTAFSEIKHMVFSILEKDEIPKLTKFMKRKHFDKKEHRWDYI
                     AGINRKIKLNLRQMFVSLDFQSDTEKPLMDAICFLKNCFNSKTALTSVDPALFPTEFI
                     SHGNKQYIDQSKVRINGKEKTIRKFSPDKYEYYVYRIIEHKLSSGDLFIKDSIRYKSF
                     EEDLIDDEKWKDKERIIEKLDIPFLKRPVDEILAELEETLETSFYDVNRRIKEGKNRY
                     IKITETGKWVLSGTRPGNDTNHPVLAEMKQVGIADILNFVDRHCNFTSSFSHIRNINT
                     SGVADRDSLFACIVSMGTNQSLAKMSEISDLTYGKLSATCDNYIRPETLKNAIDRINN
                     ATYRLPVSKHYNINELVHSSSDGQKFDSRDTVNARYSPKYFGLGKGISTVTLNANHIP
                     LNAKNIGANEHESHYVFDLLYNNTSEIVPDIHSTDTHGVNKVNFGILYPFNYGFAPRL
                     KDLRERMNDLCGFRNLSHYRDMFFKPADKVNTMLIKSQWDNILRIFVSLALKTTTQST
                     IVRKLSSYSRKNRTKEALWEFNRIIMSVYLLEYVDSADLRRDVHKALNRGESYHSLKS
                     AIFSSDKAKFKVKTVLEQQVWSECSRLLCSAVIYYNSYILSELMKDLPENERAFFSKI
                     SPAAWNHINIYGRYVFSSSTELDIDSIVRSLKDKVGSIIKEKTQKSYSSKGQIPSSLQ
                     "
     gene            complement(4425..5921)
                     /locus_tag="PL056_09570"
     CDS             complement(4425..5921)
                     /locus_tag="PL056_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47306.1"
                     /translation="MKEFLIIFLMAFLWSCTSCNEDKPQIDDLRVDNDVSGSESEEDS
                     LDENSDPDKITDLDNESDDSPDADVDTAVECLDLKIQENVIKTAFPFKDKDGRSTFCR
                     SGCDTPTETDPQCVRNIWEWDNWEKYQKYLAAEKEDPEQTNERECYSWPCELPDMKGS
                     TGGSLVSKCDRRISVNGYRSTMGAVWTHGMSNGVAGMYMTGSGAIEYDPEKDVFVKVG
                     KANGRLSFNKNRSIVLVFDSFAPDNPSYKSFVVSVLKKDGEYNYELIYDNKGHNAFMG
                     NPSFVGENWVLIQICEGDRGPCDVKYSRTDKWEWHSLDIGSVYEGNIVGNKLTFLTPD
                     ETADRQIYYCDLSKYPESYKECTKATRKDGTGKYEKGHSPRIDEDNENRLIYYMYEDE
                     GPPTLIEVNYSGENNPEYKEIEVDQYMQPHKVKGNLMMFTGWRGSKIISCWYRFDKQK
                     SYCPVINNEGMEFSTFDGKWWLWRNSIDVQIRDTECYCKEEGICPFEE"
     gene            complement(5924..>6462)
                     /locus_tag="PL056_09575"
     CDS             complement(5924..>6462)
                     /locus_tag="PL056_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47307.1"
                     /translation="KETFTRLAYVNIDSVLDFAIANGEIAMYRSSFWSAGIQIRDVDS
                     LNMKAWEYADCYTGELTADHGEFYLSCDGYEYRFEGRPDTYIDFYSLEADILELQENY
                     VYDGWTYISDGSSVKLSTLNDVPSFCGNGVIEPGEVCDGDTLSCTDLDASYYGGTAYC
                     NSLCSGYNEDSCETDDGW"
BASE COUNT         2053 a         1306 c         1177 g         1926 t
ORIGIN      
        1 cggcaacatc cctgtaattc attgcctcaa gtttttcaat tgagatcttc attaattttt
       61 tttcattaat tttaggtgta gccattttaa aaacctccat ttaaaagata gaacattact
      121 atattataaa aacagattat taagtaaaga attttttact ttttattgca gatcagagtt
      181 ttaaactttt caaatctctt caatacagaa ataacatagt tttttgtttc aggattgtta
      241 tatatccgac ccgcaagcaa cccgtccgcc gtggtcgcct ttgtttcggt tgaatgggtt
      301 ccgccgttgt agccggaaag tgactttatg aggtcgcctt tattgtatgt aagaagcgcc
      361 gcaaggaatt tgactgagca gtttattgca agttcagggt cggcgagttt attgtagtcg
      421 catttgagca gttttgcggt ttgcggcatt atctgtgtca gtccgtaagc tccggcgtga
      481 ctttttgcct gagggttgaa actgctttcc tgttcaatta tgccgagtat aagggcggga
      541 tggatataat atttcttatt attaacaata tacggatatg tctttacaga cttttcaatt
      601 aggggttttt tgcagattcc ccgaaaaacg acatagaggc ctaaaactgg ctatttaaca
      661 caatttcact tttgttaaat gaaaaagcta atgtcttata attacgaact ttttaaaaac
      721 aaaaattgac acctattaac aaatagtata tatatcgtaa ttagttgtta acttatcaac
      781 gctaaaagaa ggtgttcgca atgaagtatg ttcaaccgat cagagacatg gatttaattg
      841 aaaaactgaa ggtcatttta aagaaacaga attaccgaaa ttattttctt tttatttttg
      901 gcatcaactc aggattgcgg atatctgata ttctcagact gaaggtatcc gatgtcaaaa
      961 ataaaacaca tgtcactatt attgagaaaa aaaccggtaa ggaaaaacgc tttaagctga
     1021 acactagtct gcaggccgaa attaaagagt atatttcaaa catgtctaat gatgaatacc
     1081 tgtttacacc tgtcaatgga aagagcggga agcccataag tcgtgaaatg gcgtggatta
     1141 ttctcaacaa ggccgccgga gaactgggta tagaggaaat cggcacacat acgctccgta
     1201 agacctttgg ctatcatttt tatcagaaaa cgaaggatgt ggctctgctt cagaacatct
     1261 ttaaccactc cgctccgagt gttacgctca gatacattgg aatcaatcag gatataatag
     1321 acagtgcaat tgatgaattc agtttataat aaaagataag caggaagttt tttggaaaga
     1381 gacaacagat taaaagttct gaacgagaat gaatataatg aactgtatgg acaccccgga
     1441 ttcagcatcg aggaaagaag cgttttcttt tctctcagtg attcagaagt agaagaaaga
     1501 gataagctgc gttctgtttc agcaaaaata ttctttattc tccagactgg atactttaag
     1561 gctaaaagga tattcttcac tttggatact gagaactcta aagatgatct cctctttata
     1621 atagacagat attatccggg cttcagcatg gataacttat ctgttaccga cagagttcgg
     1681 ctgttccagc agaagatcat tgcagacata ttcggataca ggatattcaa agcacctgaa
     1741 agaaagctgt ttgccgaaag agccgcttat ctggctaaaa tacattcaaa tcctgcatac
     1801 atattcaatg aactgaaaac ctatcttgat aacgaaaaaa ttattttacc gatgtattcc
     1861 ttttttcagg acattatcgg aaaggccata tctgatgaaa ttaaaagatg cggtgaatac
     1921 atcattaaaa aaatggataa aagcctgaaa gataaaatgg aggattttct ttctgcggaa
     1981 gataatagtt tatacaatct cactctgata aaaaaagatc ctgctaattt taaaaattca
     2041 gaaatcatgc aggaagtgaa aaaacggcag tcattgaaac cactctacag ttttgctgat
     2101 aaagttctga aagaactgga gatgtccagt gagagcatta aatactattc atctctcgta
     2161 tcctattaca gtgtgtacag attaaaggaa ctgaaaagtt tagcgacaaa atatacttat
     2221 attttatgct ttatctacaa ccgttttcag cagtgtaatg ataatctgat aaattctttc
     2281 atctatcatg tgaacgatta tttcagcgat gctgacaaat atgccgtcca ggcaatattc
     2341 gagcagaaaa tgacaggaac ccgaaacctg aagaaagcgg gtaaaattct ggaactgttt
     2401 cttgatgaca acatttccgg agatacagca ttttcagaaa taaaacatat ggtcttttct
     2461 attcttgaaa aggatgaaat cccaaaactt actaaattta tgaagagaaa acactttgac
     2521 aagaaggagc acaggtggga ttatattgcc ggaataaaca gaaagataaa actcaacctc
     2581 agacagatgt ttgtcagtct tgactttcag agcgacactg aaaaaccgct gatggatgca
     2641 atatgttttc tgaagaactg ttttaacagc aaaactgctc tgacctctgt cgatccggct
     2701 ctgtttccca cagaattcat cagtcacggc aataagcaat atatagacca gtctaaagtg
     2761 agaataaacg gtaaagagaa gacaatcaga aaattcagcc ctgacaagta tgaatactat
     2821 gtctaccgca tcattgaaca caaactttct tccggggatc tcttcataaa agacagcatc
     2881 agatacaaaa gttttgagga agatcttata gatgatgaaa aatggaagga taaggaaaga
     2941 ataattgaga aactggacat tcctttcctg aaaagacctg ttgatgaaat tcttgcggaa
     3001 ctggaagaaa cccttgaaac gtcattttat gatgtgaaca gaagaataaa agaggggaaa
     3061 aacaggtata ttaaaatcac ggaaacaggg aaatgggttc tgtccggcac aagacccggg
     3121 aatgatacca accatcctgt tcttgcggaa atgaaacagg tgggcattgc ggatattctt
     3181 aatttcgttg ataggcattg taatttcact tcatcctttt cacatatcag aaatatcaat
     3241 acttcagggg ttgccgacag agactctctc tttgcctgca ttgtctcgat gggaactaat
     3301 cagagtcttg caaaaatgtc tgaaatatca gatctgactt atggcaaact ttcggccact
     3361 tgcgataact atatccgtcc agaaacgctc aaaaatgcta tagaccgcat caataatgcg
     3421 acatacagac tgcctgtgtc aaaacattac aacattaacg aacttgttca ttcaagcagc
     3481 gacggacaga aatttgattc cagagatact gtcaatgcca gatactcacc caaatatttc
     3541 ggtttgggaa aaggtatctc aactgtcacc ctgaacgcaa accatattcc gctcaatgct
     3601 aagaatatcg gagcaaatga acacgaaagt cactatgttt ttgatctgct gtacaacaac
     3661 acctcagaga ttgttccgga tattcattca accgacactc acggagttaa caaggttaat
     3721 ttcgggattt tgtatccttt caactatgga ttcgcacccc gtttgaagga tctgagggaa
     3781 aggatgaacg atctctgcgg attcaggaat ctttcacatt acagagacat gtttttcaag
     3841 ccggctgata aagttaacac catgcttata aaaagccagt gggacaacat tttaagaata
     3901 ttcgtttccc ttgcacttaa aaccacaact cagagcacaa tagtaagaaa actgagctcc
     3961 tattcccgga aaaacagaac aaaggaagct ctctgggaat ttaacagaat tatcatgtct
     4021 gtctatctcc ttgaatatgt cgattcagct gatctgagaa gagatgtaca taaagcgctg
     4081 aacaggggag aatcctatca cagtcttaaa agtgcaatat tttcgtctga caaagctaaa
     4141 ttcaaagtta agactgttct tgaacagcag gtctggagcg aatgttcaag actgctgtgc
     4201 agtgcagtta tttattacaa ttcctatatt ctgtccgaac tcatgaaaga tctgccggaa
     4261 aatgaaagag catttttcag taaaatatct ccagcggcct ggaatcatat caacatttac
     4321 ggcagatatg ttttcagcag cagtactgaa ctggatatag acagtattgt cagatctctg
     4381 aaagataagg ttggttcaat aattaaagaa aaaacacaga aatcttactc ttcaaaagga
     4441 cagatacctt cttctttgca gtaacattct gtgtctcgaa tttgcacatc aatggaattt
     4501 ctccagagcc accatttccc gtcaaaagtg ctaaactcca ttccttcatt atttataaca
     4561 ggacaatatg atttctgttt gtcaaaacga taccagcagc tgattatctt tgatcctcgc
     4621 cagcccgtaa acatcatcag attacccttt actttgtgtg gctgcatata ctgatcaact
     4681 tctatctctt tgtactcagg attattctca ccggaataat taacttctat cagtgttgga
     4741 ggaccttcgt cttcatacat ataataaatc agtcggttct cattgtcttc atcaattctc
     4801 gggctgtgac ctttctcata ctttcctgtc ccgtcttttc ttgtggcttt tgtgcattcc
     4861 ttataacttt caggatattt gctcagatca caataatata tctgcctgtc cgctgtttca
     4921 tcaggagtca agaaagttaa tttgtttcca acgatatttc cttcatacac actgcctata
     4981 tcaaggctgt gccattccca tttgtctgtc ctggaatatt taacatcaca aggacctctg
     5041 tctccttcac atatctgaat caacacccag ttttcaccga caaaagaagg gtttcccata
     5101 aaagcattgt gtcctttatt atcatagatc agttcatagt tgtattctcc gtcctttttt
     5161 aatactgaga caacaaaaga tttatatgat ggattgtctg gtgcaaagga gtcaaaaaca
     5221 agaactatgg atctgttctt attaaagctt aatcgtccat tagctttacc tactttaaca
     5281 aacacatcct tttcaggatc atattctatg gcaccactcc ctgtcatgta cattcctgca
     5341 acaccattgc tcatcccatg cgtccagaca gctcccattg ttgatctgta gccattgacc
     5401 gaaatccttc tgtcacactt tgaaaccaga cttcctcctg ttgatccttt catgtcagga
     5461 agttcacacg gccaagaata gcattctctt tcatttgtct gttccggatc ttctttttct
     5521 gctgcaagat acttctgata cttctcccag ttatcccatt cccagatatt tcttacacac
     5581 tgaggatctg tttctgtcgg ggtgtcacat ccggaacggc agaatgtaga tcttccgtct
     5641 ttatccttaa aagggaaagc ggttttaata acattctcct gaattttaag atcaaggcat
     5701 tcaactgctg tatcaacatc agcatctgga ctatcatctg attcattatc tagatctgta
     5761 atcttgtccg gatcagaatt ttcatccaaa gagtcctcct ctgattctga cccggacaca
     5821 tcattatcaa cccgcagatc atctatctgt ggtttatcct cattacatga tgtgcatgac
     5881 cacagaaatg ccataagaaa tattataaga aattctttca aatctaccat ccgtcatctg
     5941 tttcacaact gtcttcattg tagccggagc atagagaatt gcaataggct gttccaccgt
     6001 aataagaagc atcaagatct gtacaggata aagtatcccc gtcacagact tctccaggct
     6061 caataacacc gttcccgcag aaactcggaa catcgttgag tgtagaaagt tttacactgc
     6121 ttccatcaga aatgtatgtc catccgtcat aaacatagtt ctcctgcagc tcaagaatat
     6181 cagcttcaag agaataaaaa tctatgtatg tatccggtct gccctcaaaa cggtattcat
     6241 atccgtcaca cgacagatag aactcgccat gatcagctgt aagctctccg gtgtagcagt
     6301 cggcatattc ccacgccttc atgttgagag aatcgacatc tcttatctgt attcccgcac
     6361 tccagaacga tgatctgtac atagctatct caccgttcgc tatggcaaaa tcaagtactg
     6421 aatcaatatt gacataggca agacgggtga atgtctcctt gt
//
LOCUS       DAOOYB010000164         6374 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_14003_length_6374_cov_14.917075, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000164 DAOOYB010000000
VERSION     DAOOYB010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6374
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14003_length_6374_cov_14.917075"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="PL056_09580"
     CDS             <1..654
                     /locus_tag="PL056_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47308.1"
                     /translation="GDTGNTGNTGNTGDTGNTGNTGDTGNTGDTGNTGNTGNTGNTGN
                     TDNTGNTGNTGDTGVTPDYELSFTGVINTADSTNKTGGTGTAGFSFKATDYVYTTQNL
                     MYITTPFANTETQTPTYSITWIEPLSGGISTTIKFFAIYIPQSQNNGEYPSGGTGSMV
                     IFGDLQQGSSEFTLKCVRAIGDDTYMDYEKSGNNLTVIAEGNLAEPAPGSGFTVCTD"
     gene            complement(635..1483)
                     /gene="prmC"
                     /locus_tag="PL056_09585"
     CDS             complement(635..1483)
                     /gene="prmC"
                     /locus_tag="PL056_09585"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HPM47309.1"
                     /translation="MTLTISNLILKYSKELENISDSPRLDVEIMLSRVLNLSRFDLLM
                     KANDSTDIVDEKRFAELLKRRMNFEPVAYIINEKPFFEDVFYVDSRVLIPRPETEFLV
                     MEAITHLKSYEKMPAVLDICCGSGCVGLSILRVIDCDLSMSDISKDALDVTSVNVGKL
                     FPQRDDIKLIQSDLFENVNEKYDVITANPPYLSKKDMAEFAKNDLRYEPENALSGGES
                     GFEFTEKLLNEAGEYLNHDGIIAVELGFEGSKFIKDVYGKIELHKIKKDYNGIDRVAV
                     FSRCRR"
     gene            complement(1464..2573)
                     /gene="prfA"
                     /locus_tag="PL056_09590"
     CDS             complement(1464..2573)
                     /gene="prfA"
                     /locus_tag="PL056_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HPM47310.1"
                     /translation="MLDIRDKLSEVYESYLRMERELSRPESTSDMKKYTKMMKEYKHL
                     KKVSDKFLEWTKLEHEMSGHRQMLASEKDPDLVEMIRSELPDLESEYDRLYEELNVLL
                     IPRDPLDEKNAILEIRAGTGGDEAALFAGEVYRMYCRYAEMKRWKVEVMSMSSMEGGG
                     IKEVISIVTGEDVYGRLKYEGGVHRVQRVPVTESQGRVHTSAITVAVLPEVDEIDEVT
                     IDEKDLKIDIYRASGAGGQHVNKTDSAVRITHLPTGIVVAMQDERSQIQNRVKAMKII
                     ESKVLQMEREKAEKEISSKRKSLVGSGDRSEKIRTYNFPQGRVTDHRIKLTLYRINDI
                     MEGSLDMLIDPLVNYQNALLLGRSVEEFGNDSDDQ"
     gene            complement(2646..2864)
                     /gene="rpmE"
                     /locus_tag="PL056_09595"
     CDS             complement(2646..2864)
                     /gene="rpmE"
                     /locus_tag="PL056_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011135299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HPM47311.1"
                     /translation="MKDKVHPKLEVITVKCSCGAEIVTRSTKKEGYNIEICSSCHPFY
                     TGKTKIVDTAGRIEKFNAKYKRDTYTKK"
     gene            complement(2970..4289)
                     /locus_tag="PL056_09600"
     CDS             complement(2970..4289)
                     /locus_tag="PL056_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HPM47312.1"
                     /translation="MKRSIKPAEGKLMVFIPGMGAITSTVLAGIFNIRKGLSKPIGSV
                     TQMGHIRLGKRTENKSPLIKEFVSLASIDALEFAGWDIFEDDMYEAAKKADVVLPKHL
                     EPVKEEMKAIKPMSAVFYHDWVKNLDGKNIKKGKNHMDLANQLMDDMKKCMAEKGCSR
                     AVMVWNASTEAYHKPCEIHETLEAFEKALNDNHPEVAPSQIYAYAALKMGFPYINGAP
                     NYTVDIKAIQELAEKNNVPIAGKDWKTGQTLMKTILAPGFKTRMLGLSGWFSTNILGN
                     RDGEVLDDPANFKSKEVSKLSVLDTILEPENYPDLYGKFYHKVRINYYPPRGDAKEAW
                     DNIDIFGWLEMPMQIKVNFLCRDSILAAPVVLDTVLFIDLAARAGFHGIQEWMSFFFK
                     SPMVAQGLYPEHDIFIQQTKMKNTLRFMMGEELITHLGNEYYDYMKE"
     gene            4549..>6374
                     /gene="htpG"
                     /locus_tag="PL056_09605"
     CDS             4549..>6374
                     /gene="htpG"
                     /locus_tag="PL056_09605"
                     /inference="COORDINATES: protein motif:HMM:NF003555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HPM47313.1"
                     /translation="MAKKSFKAETKKLLDLMINSIYTNREIFLRELISNCSDAIDKLK
                     FKSLTAPELIKDGGDFEIWIETDKEQKTLTISDNGIGMTHDEVIENIGTIAKSGSKEF
                     FEKLSESDDNKNAAELIGQFGVGFYSAFMVAEKVKIRTRSAEAELSEGVIWESTGDGN
                     YTVEKAEVEKRGTEITLYLKEDILKEIQLLDHDELEELVTRYSDFIRYPIKMNVEKET
                     VIEEKTLNSMKPIWNREKSDVTEEEYKEFYRHAFHAYDEPVDVIHTKGEGTVEYTALI
                     FIPSQIPFNFYSSHFERGMKLYSKQVFIMDKCKELVPEYLGFIRGIVDSPDFSLNISR
                     EILQHSSQLKVISKNLEKKILGALKYMLENDREKYIKFWKEFGKAIKAGVYSDFGMNR
                     DKLQDLLLFESSHSDELTTLDEYVKRMPEGQKEIYYAAGRDKDAVKNTPQMEAISEKG
                     YEVLYFIDKIDEFASAVIINYKEKPLRNISAADLDFDSEEEKKEKEEKRKEKETESKS
                     LLETLKKHLENEVKEVRLSSRLKNSAVCLVSGSDGISINMEQVLSEAAMSNMPAPKAT
                     RILEINPEHRIFTKLNEIYTSNPESEKLKNFAQVLYNQALLM"
BASE COUNT         1957 a         1382 c         1214 g         1821 t
ORIGIN      
        1 ggcgacactg gcaacactgg caacacaggc aacactggcg acacaggcaa cacaggcaac
       61 actggtgaca ccggaaacac cggcgacact ggaaacacag gtaacacagg caacactggt
      121 aatacgggca atactgataa tacgggcaac accggaaaca ccggcgacac aggcgtgacg
      181 cccgactatg aattgagttt taccggtgtg ataaataccg cagattccac aaataagaca
      241 ggcggaacag gcactgccgg tttcagtttt aaagccaccg actatgttta cacgactcag
      301 aacctgatgt atataacaac cccttttgca aatactgaaa ctcagactcc aacatattcg
      361 atcacatgga tagaacccct ttcgggaggc atcagcacaa caataaaatt tttcgcaatc
      421 tatattccgc agagccagaa caatggagaa tatccttccg gcggcacagg atcaatggtc
      481 atattcgggg accttcagca gggaagcagc gaatttacac ttaaatgcgt aagggcaata
      541 ggagatgaca cctatatgga ttacgaaaaa tcgggaaaca acttaactgt tattgctgaa
      601 ggaaatcttg ctgaaccagc tccgggaagc ggatttaccg tctgcaccga ctgaaaacag
      661 cgaccctgtc tattccgttg taatcttttt ttattttgtg aagttcaatt tttccgtaaa
      721 catctttaat gaactttgat ccttcaaatc caagttcaac agcaatgatt ccgtcatgat
      781 ttaaatattc acccgcctca tttaaaagct tttcggtgaa ttcaaacccg ctctctcctc
      841 ccgaaagtgc attctcaggt tcgtatctga gatcattttt tgcaaattca gccatatcct
      901 tttttgaaag atatgggggg tttgcagtta tcacatcata cttttcatta acattttcaa
      961 acagatcgct ctgaataagc ttaatatcat ctctttgagg aaaaagtttt ccaacattta
     1021 cagaggtcac atccagagca tctttagaaa tatctgacat cgaaagatcg cagtcaataa
     1081 ctctcagaat gctgagtccc acacagccgc ttccacaaca gatatcaagc accgccggca
     1141 ttttctcata acttttcaga tgggtaattg cttccataac aagaaattct gtttcaggtc
     1201 tcgggatcag aacgcgggaa tcaacataaa aaacatcttc aaaaaacggt ttttcattaa
     1261 ttatgtatgc aaccggctca aaattcatcc ttctttttaa aagttccgca aatctttttt
     1321 catcaacaat atctgtcgaa tcatttgctt tcattaaaag atcaaatctc gaaagattca
     1381 aaacacggga gagcatgatc tctacatcga gccgcggaga atcgcttatg ttttcaagct
     1441 ctttagaata tttgaggatt aaattactga tcgtcagagt catttccgaa ctcttcgaca
     1501 ctgcgaccca gcaggagcgc attctgataa tttacaagag gatctataag catgtcaagg
     1561 gatccttcca taatgtcatt gatcctataa agagtaagct ttatgcgatg atcggtaaca
     1621 cgaccctgcg gaaagttgta agtccttatt ttttctgatc tgtctccact tcctacaagc
     1681 gatttcctct ttgaacttat ttccttttca gctttctccc tttccatctg gagaactttt
     1741 gattctatta ttttcattgc cttgactctg ttctggatct gacttctttc atcctgcatt
     1801 gcaacaacga tccctgtcgg caggtgcgtt attcttacag cactgtctgt cttgtttaca
     1861 tgctgtccac cggcaccact cgctctgtaa atatctattt tcagatcttt ttcatcaata
     1921 gtcacttcat caatttcatc aacctcgggc agaaccgcaa cagttatcgc cgatgtatgc
     1981 accctgcctt gcgattctgt caccggaaca cgctgtaccc tgtggacgcc cccctcatat
     2041 ttaagccgtc cgtaaacatc ttcaccggtt acaattgata ttacttcctt tattccgccc
     2101 ccttccatcg aactcattga catgacttct actttccacc ttttcatttc ggcatagcgg
     2161 cagtacattc tataaacttc tccggcaaaa agagccgcct cgtctcctcc tgttcccgcc
     2221 cttatttcaa ggatcgcatt cttttcatca agaggatctc tcggtatcag aagaacattc
     2281 agttcttcat aaagccggtc gtattcagat tccagatcag gaagctcact tcttatcatt
     2341 tcaacaagat cggggtcctt ctcagaagca agcatctgtc tgtgtcctga catttcatgt
     2401 tcaagtttag tccattcaag aaatttatct gatacttttt tcaggtgctt gtattccttc
     2461 atcatttttg tatatttttt catatctgat gtagattcag gcctagaaag ctccctctcc
     2521 attctcagat aactttcata tacttcgctg agtttgtctc ttatatcaag catctcgccc
     2581 ccggtaaata aaaaaatccc gccaactcct tacaagaatt gacgggaatt aaaaaagccg
     2641 gaccgctatt ttttggtgta ggtgtccctt ttgtattttg cgttgaattt ttcaattctt
     2701 ccggcggtat caactatctt tgttttgccg gtgtagaaag gatgacagct ggaacagatt
     2761 tctatgttgt agccctcttt tttggttgaa cgggttacaa tttccgcacc gcatgaacat
     2821 ttaactgtta tcacttcaag cttcggatgt actttgtcct tcatcgtaat ctcctaaaat
     2881 aattagagaa caaaaaccgg atgaatggta ttcatgacat tgtaaaaatc aagatttatt
     2941 taaaaatgaa ctctaagatg ctgaaatcac tattctttca tataatcgta atattcgttt
     3001 ccaaggtgtg tgataagctc ttcgcccatc atgaacctca aggtgttttt catttttgtc
     3061 tgctgaatga agatgtcatg ttcagggtag agaccttgtg caaccatcgg tgatttgaaa
     3121 aagaagctca tccattcctg aattccatga aaaccggcac gggccgcaag atcaatgaaa
     3181 agaactgtgt caagaacgac cggagcggca agtattgagt ctctgcagag gaaattaacc
     3241 tttatctgca tcggcatttc aagccatccg aaaatatcga tgttgtccca tgcttctttt
     3301 gcgtctcctc tcggcggata gtagttgata cggactttgt gatagaactt gccgtaaaga
     3361 tccgggtaat tttccggctc aaggatcgtg tcaagaacag aaagttttga aacttccttg
     3421 cttttaaaat tcgcaggatc atcaagaact tcaccgtcgc ggttgcccag aatgtttgtt
     3481 gagaaccatc ccgaaagacc cagcattctt gtcttgaaac cgggggcaag tattgtcttc
     3541 atcaatgtct gtcctgtctt ccagtccttt cctgctatcg gaacattgtt cttttcagca
     3601 agttcctgga tcgccttgat gtcaactgtg tagttgggag cgccgtttat gtaaggaaat
     3661 cccattttca gtgcggcata ggcatatatc tggctcggag cgacttcagg atgattatca
     3721 ttaagagctt tttcaaatgc ttcaagagtt tcatgaattt cacatggttt gtgatacgct
     3781 tcagttgatg cattccaaac cataacagct cttgagcacc ccttttcagc catgcacttt
     3841 ttcatgtcat ccataagctg attggcaaga tccatgtgat tcttgccttt tttaatgttt
     3901 ttcccgtcca gattctttac ccagtcgtga tagaaaacag cactcatcgg ttttatcgcc
     3961 ttcatttcct cttttactgg ctcaagatgt ttgggaagaa cgacatctgc cttctttgcg
     4021 gcttcgtaca tatcgtcttc aaaaatgtcc cagccggcaa attcaagtgc atcaatcgat
     4081 gcgagtgaaa caaattcttt aataagaggt gatttgtttt ctgttctctt gccaagacgg
     4141 atgtggccca tctgtgttac tgaaccgatc ggctttgaaa gaccttttct gatattgaaa
     4201 ataccagcaa gaactgtaga agtaatggct cccataccgg gaatgaacac cataagtttg
     4261 ccctctgcgg gcttaattga acgcttcatg aaaatcctcc ggaaaaataa gtttatgtaa
     4321 aaaatcacaa aacaaaaaag cgggacagga tagcaattac cgtgcctggc ggaaacatta
     4381 gtgattttaa atccataagt caaagaattg ttatctgtcg taagaacaat ttgaatttgc
     4441 cggcaaagtg taaaaaaatt ttacaacgac ttaaacgact cctcctattg actttattga
     4501 atcggttttt atatagacct ctgattttta acctgtgcgg aggaaattat ggcaaagaaa
     4561 agttttaagg cggaaacaaa gaagcttctc gatcttatga tcaattcaat ttacacaaat
     4621 cgtgaaattt ttcttagaga acttatttca aactgctctg atgcgatcga caaactcaag
     4681 ttcaaatcgc tgaccgctcc tgaattaata aaggatggcg gtgattttga aatatggata
     4741 gagacggaca aagagcagaa aacactcacc ataagcgaca acggcatcgg aatgactcat
     4801 gacgaagtta ttgaaaacat tggaacaatc gcaaaaagcg ggtcaaaaga gttttttgaa
     4861 aaactttctg aatcagatga taacaaaaac gcagcggaac ttatcggcca gttcggagta
     4921 ggtttctaca gtgctttcat ggttgctgaa aaagtgaaaa taagaactag gtcggctgaa
     4981 gctgagttgt ctgaaggagt tatctgggaa tcaaccggag acggaaatta tacagttgaa
     5041 aaagctgaag ttgaaaaaag aggaacagaa ataacgctct atctcaaaga agatatttta
     5101 aaggaaattc agcttcttga tcacgacgaa cttgaagaac ttgttacaag atacagcgat
     5161 tttataagat atccgataaa aatgaatgtt gaaaaggaaa ctgtcatcga agaaaagacc
     5221 ctgaactcaa tgaaaccgat atggaataga gaaaaatcag atgtaacaga agaagaatac
     5281 aaggaatttt acagacacgc gtttcatgct tacgatgaac cggtcgatgt gatccacaca
     5341 aaaggtgaag gaacagttga atataccgct ctcatcttca ttccgtctca gatcccgttc
     5401 aacttctatt caagtcattt tgaaaggggg atgaaactct attcaaaaca agttttcatc
     5461 atggataaat gcaaagaact tgttccggaa tatcttggat ttattagagg aatagttgac
     5521 agccccgatt tttcattaaa tatttcaaga gaaatcctcc agcactcttc tcagcttaaa
     5581 gtgatctcaa agaaccttga aaaaaagatt ttaggcgcat tgaaatacat gcttgaaaac
     5641 gaccgcgaaa aatatattaa gttctggaaa gaatttggaa aagcgatcaa agcaggagtt
     5701 tattcagatt tcgggatgaa cagggacaaa ctgcaggacc ttcttctttt tgaatcttct
     5761 cacagtgacg agctcaccac tcttgatgaa tatgtaaaaa ggatgccgga aggccagaag
     5821 gaaatatatt atgccgcagg cagagacaaa gatgccgtaa agaacactcc tcaaatggaa
     5881 gcaatcagtg aaaaaggata tgaagttctc tatttcattg ataagattga tgaattcgca
     5941 tcagccgtca taatcaatta caaggaaaaa ccgctccgca acatttcagc agccgacctt
     6001 gattttgatt ctgaagaaga aaaaaaggaa aaagaggaaa aaagaaaaga aaaggaaact
     6061 gaaagcaagt cactccttga aacactcaaa aaacaccttg aaaacgaagt gaaggaagta
     6121 cggctctcat cccgcctcaa gaacagtgca gtatgccttg tgagcggaag tgacgggatc
     6181 agcatcaaca tggagcaggt tctttctgaa gccgcaatgt caaacatgcc cgcacccaaa
     6241 gcaacaagaa ttcttgaaat aaaccccgaa cacagaattt tcacaaaact taacgaaata
     6301 tatacctcaa acccagaatc tgaaaagttg aaaaactttg cacaagtcct gtataatcag
     6361 gcacttctca tgga
//
LOCUS       DAOOYB010000165         6373 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_14006_length_6373_cov_11.952358, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000165 DAOOYB010000000
VERSION     DAOOYB010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6373
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14006_length_6373_cov_11.952358"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            165..590
                     /locus_tag="PL056_09610"
     CDS             165..590
                     /locus_tag="PL056_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47314.1"
                     /translation="MRSEAQMIRLKRSFLLTILILTLIRLALPHENKAVLCFSNDGQL
                     SIRETQHSLCKSSHHDPELSEIEFHDHDHCTDVEIPSLSAKINTTRILDCLAYEPFLN
                     FKIVLQSGTLFEKTPKLQSLVDHGRQKRLNIISKTVLIC"
     gene            650..1879
                     /locus_tag="PL056_09615"
     CDS             650..1879
                     /locus_tag="PL056_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47315.1"
                     /translation="MKNSVFSLSMFFIVAFPGILHCDDEVELIKSFLSTPYEVELIKT
                     GKKAVTAKSKHDPYMKNPSFSMSIEKPFDDSSYAFKFAEFGFEFDVSGKYILGKEVAG
                     FKKLRAGEQIKASLVESVANFRALLAKFFYLNRKKEIIKVFELKIETLSKELSILVEG
                     KNSSKFDLMRIESQVLSHKRKSLEIESELAAVRSQIQSFCGIEIDELSLQEPELIEFE
                     ELKRRAFLENPQIKQIHFNLSESEKEMAVEKRKTIPDPGLQIGFIHEKMQGVSLGFGI
                     DIGISFDLPFFDFNRKGVAESVYENQQKKMDLFEKKFFVDLQISELYKKADHLIKGFI
                     PDQNKDDILEKSFFLYKAGEISVSDLISTLYEVEEIHFSEIEIKEKIHFLTLQLYAAA
                     GFFENEKINDLIGGAVK"
     gene            1876..3069
                     /locus_tag="PL056_09620"
     CDS             1876..3069
                     /locus_tag="PL056_09620"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPM47316.1"
                     /translation="MKTSAFILFINILFAGCTHDHEDHSGHDHSRKEEPSSQHHGNHD
                     EEIKSIHFNKEKQVLAGVETVMPEMSEVMERVSVPGIIKADPRYSFTISSTSRGIFSY
                     SASGDLLHEGKLVKKGEILAVIYPVAQQEHWTQIEQAHNLAVVEKEFAQNEYERINDL
                     AQKGVVPEKELLIAKNNLEASAQNLKFAEKRASQLQGGREKSIQIKASVSGVLRKIFI
                     KSGMLVESGETLFEIVVPDKLMINGEILKGDIPSGSKIMSVSVKASDSEMTVITSADI
                     KNRTYTFDYTTKSSEIVFPFESSGFYIGENVEIILRTGKGVPSLTVTSSSVIEIATRP
                     HLVIMKETEIFEIIQVDAGADDGLKVEILKGLKETDRVVSKGAFEIYAASRTESMDAH
                     AGHNH"
     gene            3082..6162
                     /locus_tag="PL056_09625"
     CDS             3082..6162
                     /locus_tag="PL056_09625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPM47317.1"
                     /translation="MWDYIIKWSLNNRTIVFAIAVSVVISGFYATYTSKLDALPDITA
                     PTVTVVTDAGGLAPEEVEQLVTFPIESALNGAPGLRRLRSRSSVGMSIVWAEFEWNTD
                     QYKARQIISERLQLVLNQLPSEIKTPVMAPISSIMGEIMVIGLTGDKNVSPMELRDIA
                     EWTIRKRVLGIPGVAQVTPIGGEIRQVEIILNPEKMRQFKVGHQEVIERIKNATVNSS
                     GGFFVSGYSEYIVRGLGRADDFEDFGKTVIKKSGNAQVLLKHIADIGYGHPPARGSAS
                     INGDPGVLLTIQKQPEADTLDLNEKINEELDTLSKTLPENVKLFREGFNQSSFINTAI
                     SNVTRHLFESSVLVIFVLFLFLASKRATLISLAALPLSLFSGIIVLRIMGESINTMTL
                     GGFAIAIGALVDDAIIDVENGYRRLRLRAALPEDQRDSLFDNVYKSSVEIRSSIVYAT
                     FIIAIVFVPLFFLTGLEGKLMRPLGLAYVVSIISSLLVATTVTPALCMVLLRNVEPYE
                     EKKESFLTIILKKVYTPILKFSLKNAGKVTVSAVCITFASVVLFLHFGRDFMPKFNEG
                     SFSITAATVPGVSLEQSTKMINRLDKAVYGLDFVKSVARRTGRSDEKDEHGHDVQASE
                     MDIIIDNSKISRTEAEKIIRETAEKIPGLKVTVTQPISFKIAHMNTGAEASIVLKIFG
                     EDLGKLRDLAQEVEENVHEIDGLVDLNIEQLTDVPVLNIKPDLNKLAYFNVTSGEFTS
                     FVQTAFSGSEVGKWWEKQRSYDMVVKYPKSFQTGINSMKRVPFDKDGEQFITLSTLAQ
                     IEKTISPNVIQRENSHRRITVTANVAGRDGRSVVNEMKEQIEKNVEFPQGYYPVFGGD
                     FSSEEKASRTILWLSIFAIIIIILMLTVLFKSLSDALIVVVNMPLSLIGGTIAVLFMN
                     GIMTVATLVGFITLFGIASRNGIMMISHYKYLEQVEKCSKDEAIFRGSIERLIPIMMT
                     ALATAFALIPIVIGLDEPGSEIQAPMALVILGGITTSTFLNMVVLPSIYKLTKA"
BASE COUNT         2057 a         1042 c         1366 g         1908 t
ORIGIN      
        1 taccaacaga acccattcga taaaatataa accggaagat tttaccaaca taaaagcatt
       61 cagaagaata gtttttgatg caattctttt agaagcaggg aaatgaaaaa agcaattgct
      121 tcttgacaac catgctaaac actatattgt ttttgaggta tttgatgaga tcagaggctc
      181 aaatgattcg actgaaaagg tcttttctgc tgacaattct tattttaacg cttatacgat
      241 tggctcttcc gcatgaaaac aaagcggttc tctgtttcag taacgacgga caactttcaa
      301 ttcgcgaaac acagcattct ctttgcaaaa gctctcacca tgatccggag ttgtcagaaa
      361 tagagttcca tgatcacgac cattgcactg atgttgaaat tccttctctt agtgccaaaa
      421 tcaatacaac aagaattcta gattgccttg cttatgagcc tttcttgaac tttaagattg
      481 ttttgcaatc cggcactctt tttgaaaaga ctcctaaatt acaaagcctt gttgatcacg
      541 gaagacaaaa aagactgaac ataatttcca aaacggtctt aatctgttag actcaatata
      601 acacatcaat atgattagct ttttattgtt ttaaaaaggg atttttaata tgaaaaactc
      661 agttttctct ctatctatgt tttttattgt ggctttcccg ggtattttgc attgtgatga
      721 tgaagttgag ctgataaaaa gctttttgag caccccttat gaggtggagc ttatcaaaac
      781 aggcaaaaaa gcggtaactg ccaaaagtaa gcatgatcct tatatgaaaa acccttcctt
      841 ttcaatgagt attgaaaagc cttttgatga cagttcctat gcttttaaat ttgctgagtt
      901 cggttttgaa tttgatgttt caggcaaata tattctggga aaagaagttg ccgggtttaa
      961 aaaattgcgg gccggagagc agatcaaagc atcattggta gaaagtgttg caaatttcag
     1021 agcacttctg gcaaagtttt tttatctgaa caggaaaaag gagataataa aagtatttga
     1081 gttgaaaata gaaacacttt caaaagagct ttcaattctt gtcgaaggaa aaaacagctc
     1141 aaagtttgat ctgatgagga tagagtcgca ggttttgtcg cacaaaagga aatcactgga
     1201 aatagagtct gaacttgcag ctgtaaggtc acaaatccaa tctttttgcg gaattgaaat
     1261 agatgagttg tctttgcaag agcccgaact gattgagttt gaagaactga aaagaagagc
     1321 tttcctggaa aatccccaaa taaaacaaat tcattttaat ttgtcagagt cggaaaaaga
     1381 aatggcagtt gagaaaagaa aaacaattcc cgatcccggt cttcaaatag gttttatcca
     1441 tgaaaaaatg cagggagtca gtttaggatt cggaattgat ataggaataa gttttgatct
     1501 gccttttttt gattttaaca gaaagggtgt tgctgaatca gtttatgaga atcagcagaa
     1561 aaaaatggat ctttttgaaa aaaaattctt tgtggatctg caaatatctg aactttacaa
     1621 aaaagctgat catcttatca aagggtttat tcctgatcaa aacaaagacg acattttgga
     1681 gaaaagtttt ttcctttaca aggcaggtga gatttcagtg agtgatctga tctcaacact
     1741 ttatgaagtt gaagagatcc atttttccga aattgaaata aaagaaaaga tccatttttt
     1801 aacacttcaa ctttatgcgg cagcgggttt ttttgaaaat gagaaaatta atgatttaat
     1861 cggtggagct gtaaaatgaa aacatctgca tttattcttt ttattaacat actttttgcg
     1921 gggtgcactc acgatcatga agatcattcc ggtcacgacc acagcagaaa ggaagaaccg
     1981 tcatctcaac atcacggcaa tcatgatgag gaaataaaaa gtattcattt taacaaagaa
     2041 aaacaggtcc ttgccggggt tgaaacagtg atgcctgaaa tgtctgaagt aatggaaaga
     2101 gtgtctgtcc ctggaattat aaaagctgat cccagatatt cattcacaat ctcttcaact
     2161 tcaaggggaa ttttttcata tagtgcatcc ggtgatcttt tgcatgaagg aaagttagtg
     2221 aaaaaagggg aaatccttgc tgttatttat ccggttgctc aacaggagca ctggactcaa
     2281 atagagcagg ctcacaatct tgctgttgta gaaaaagagt ttgcacagaa cgaatatgaa
     2341 agaataaacg atcttgctca aaaaggggtt gtgcctgaaa aagaactttt aattgcaaaa
     2401 aataatcttg aagcatctgc tcaaaatctg aaatttgcag aaaagagagc atctcaactt
     2461 cagggaggaa gagaaaagtc cattcagata aaagcttctg tgagcggagt tttaagaaaa
     2521 atatttatta aaagcggaat gcttgttgaa tcaggagaaa ctctttttga aattgttgtt
     2581 ccggataaac ttatgatcaa tggtgaaatt ttaaaaggag acattccgtc cggctcaaag
     2641 atcatgtcgg tttcagtaaa ggcaagtgac agtgaaatga cggtgatcac cagtgcagat
     2701 attaaaaaca ggacatacac atttgattac accacaaaat catcagagat agttttccct
     2761 tttgagagtt caggattcta tataggagaa aatgtagaaa ttattcttag aacaggaaaa
     2821 ggtgtgccgt cgcttactgt tacttcttca tctgtgatag aaatagcgac acggcctcat
     2881 cttgttatta tgaaagaaac ggaaattttt gagataattc aggtggatgc cggagccgat
     2941 gatggtttga aagttgaaat tttaaaaggt ctgaaagaaa cggaccgagt tgtttcaaaa
     3001 ggggcttttg agatttatgc agcaagcaga acagaatcaa tggatgctca tgccggacat
     3061 aatcactagc tggagaaaaa aatgtgggat tatataatta aatggtcatt gaataacagg
     3121 acaatagttt ttgccattgc ggtttctgtt gtgatttcag ggttttatgc gacatatacc
     3181 agcaagcttg atgcattgcc ggatataaca gcaccgactg ttaccgtggt taccgatgca
     3241 ggcggacttg cccccgaaga ggttgagcag cttgtaactt ttccgattga aagtgctttg
     3301 aacggtgccc cgggtttaag gagactcagg agcagatcat ctgtcggaat gtcaatcgtg
     3361 tgggctgaat ttgaatggaa caccgatcag tacaaggcaa ggcagattat ttcagaaaga
     3421 cttcaattgg ttttaaatca gcttccttca gagatcaaga cacctgtgat ggctcctatt
     3481 tcatcaatca tgggagaaat aatggtaata gggctgacag gcgataaaaa tgtttcaccg
     3541 atggagctta gagacattgc agagtggacc atcagaaaaa gagtgctcgg aattccggga
     3601 gttgctcagg taactccgat aggcggagag atcaggcagg ttgaaattat tctgaatcct
     3661 gaaaaaatga ggcagtttaa agttggacat caggaagtga tagaaaggat taaaaatgca
     3721 accgtaaatt caagtggagg tttttttgtc agcggctata gtgaatatat tgtaagagga
     3781 ctcgggaggg ctgacgattt tgaagatttc ggaaagaccg taattaaaaa gagcggcaat
     3841 gcacaggtgt tactgaaaca tattgctgat attggatatg gacatcctcc agccagagga
     3901 agtgcttcga tcaacggaga tcccggagtt cttctcacaa ttcaaaaaca gccggaagct
     3961 gacacgcttg atctgaatga aaaaatcaat gaggagcttg acaccctgtc aaaaacactg
     4021 cctgaaaatg ttaagctttt tcgcgaaggt tttaatcaga gcagctttat taatacagca
     4081 atttcaaatg ttaccagaca tctttttgaa agctctgttc ttgttatttt tgttctattt
     4141 ctttttcttg caagcaaaag ggctactttg atatcgcttg cagctttacc gctttctctt
     4201 ttttcaggaa taatagttct aaggatcatg ggggaatcta ttaacacaat gactctggga
     4261 ggctttgcaa ttgcgatcgg agcattggtt gatgatgcta ttattgatgt cgaaaatgga
     4321 tacaggagac ttcgtttaag ggcggcactg cctgaagatc aaagggacag tctttttgat
     4381 aatgtctata agtcgtctgt agaaataagg agttctattg tttatgccac atttatcata
     4441 gcgatagttt ttgttccgct tttttttctt acaggtcttg aaggaaaact aatgcggcct
     4501 ctaggcttag cttatgtcgt ttcaattatt tcttctcttc ttgttgcaac tactgttaca
     4561 cctgctcttt gtatggttct tttaagaaat gtagagcctt atgaagaaaa gaaggaaagc
     4621 tttctgacaa ttatcctgaa aaaagtttac acgccgatct taaagttttc tttaaaaaat
     4681 gcaggaaagg tgactgtcag tgcagtttgt ataacttttg cttctgttgt ccttttttta
     4741 cacttcggca gagattttat gcctaaattc aatgaaggga gtttttcaat tactgcggcc
     4801 acagttcccg gagtgtctct tgagcagtca acaaaaatga taaatcgtct ggataaagct
     4861 gtctatggcc ttgattttgt gaaatcggtt gcccgcagaa cgggaaggag cgacgaaaaa
     4921 gatgaacatg gacacgatgt tcaggcaagt gaaatggata taattattga taattcaaaa
     4981 atcagcagaa ccgaagctga gaagataatt agagagactg ctgaaaaaat tcccggactt
     5041 aaagtaaccg tcactcagcc gataagtttt aaaatagctc atatgaacac tggagcagaa
     5101 gcatcaattg ttcttaagat ttttggagaa gatcttggaa aactgagaga tctggctcag
     5161 gaagttgaag aaaatgttca tgaaatagac gggcttgtcg atctcaatat tgaacagttg
     5221 acagatgttc ctgttcttaa tatcaagcct gatctgaaca aacttgccta ttttaatgta
     5281 acttcaggag agtttacatc atttgttcaa actgcttttt caggttctga agttggaaag
     5341 tggtgggaaa agcagagatc ttatgatatg gttgttaaat atccaaagtc attccagact
     5401 ggtataaaca gcatgaaaag agtgccgttt gataaagacg gagagcagtt cataactctt
     5461 tccacacttg cacaaattga aaaaacgatc tctcccaatg taattcaaag agagaattct
     5521 catcgaagga taacggtgac ggctaatgtt gccggaagag atggaagaag tgttgtcaac
     5581 gaaatgaaag agcagatcga aaaaaatgtg gagtttccgc aaggatatta tccggttttc
     5641 ggaggagatt tcagcagcga agaaaaggcg agcagaacaa ttttatggct ttcaattttt
     5701 gcgataatca tcatcatatt aatgctgaca gtgcttttca agtcattaag cgatgcattg
     5761 atagtagtgg tcaacatgcc tctttctttg atcggaggta cgatcgcagt tctttttatg
     5821 aacgggataa tgaccgttgc gacacttgta ggatttataa ctcttttcgg aatagcttca
     5881 agaaacggaa taatgatgat atctcactac aaatatcttg aacaagttga aaaatgttca
     5941 aaagatgaag caatcttcag aggaagtatt gagcggctaa tacccataat gatgacagcg
     6001 cttgccacag catttgcatt aattccaata gttatcggac ttgatgagcc gggaagtgaa
     6061 attcaggcac cgatggctct tgttattctt ggcggaataa caacaagcac ttttttaaat
     6121 atggttgtgc tgcccagtat ttataaattg actaaggctt gacggttttt atttgtttga
     6181 agaacataat tgtttggaag ttttcaactt atcttgattt tgccgagctt ttttagcaga
     6241 atttctttga gttaacggtc tgcgggatgg ttgtgaatga aaatgaaaaa tatcatcgta
     6301 ctgcttctta ctgttttctt ctttttttca gcttgtgata tcgaaaaaag atctgttgcc
     6361 attgatgaat ctg
//
LOCUS       DAOOYB010000166         6327 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_14140_length_6327_cov_13.931122, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000166 DAOOYB010000000
VERSION     DAOOYB010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6327
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14140_length_6327_cov_13.931122"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1015)
                     /gene="recF"
                     /locus_tag="PL056_09630"
     CDS             complement(<1..1015)
                     /gene="recF"
                     /locus_tag="PL056_09630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HPM47318.1"
                     /translation="MKISQIALINFRNLEKLDLTFSDKMIINGKSGAGKTSIIEASYI
                     LLSGKSFKTHEMKECIKKGEDNFFIKCHTEDFNSFFRDISVGYNYDGSRKILIDGISS
                     SRKELMNIVFPVMHAPENMELINGGPKGRRDFIDRICFMDDSSYFDQLVEYYRFIKQK
                     NAALKKGSVKAVEYLNHAAVELIEKIRDKRKNGCNRINGKIMSLSEKFFPGCSIALNP
                     YIEENTAEKLSLKLEKEMDKGFSLYGPHLDPLNINIDTGSLKNNLSMGETYLLSFILK
                     LSEIAIYEEKNIYPVFFIDDIFVFLDEEKKKKLFKMITEMKNQVIMTSSVEKNSGHDE
                     IVHI"
     gene            complement(1026..2168)
                     /gene="dnaN"
                     /locus_tag="PL056_09635"
     CDS             complement(1026..2168)
                     /gene="dnaN"
                     /locus_tag="PL056_09635"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HPM47319.1"
                     /translation="MIKLSFKKEELLKALDYCSSSIEKKNTMPILSHILFKFKGKECT
                     LNATNLETTVVVKVPISENVDDGKITVPAKYIHDICKVARGDNILFKYDTANNILDIT
                     IEKTKYTVPCSDSGDFPAIPETDKKFNDIEINKLISYYKKLQFSMTEHNVNKAYSGVL
                     ITKVSDPNGNFIEMVTTDIHRLSVLSIKNFSAEIDGLESGLVIPGKNFFEISKIFADC
                     ETAKISVDDDKIFISSENVLFISRLLKNEFPNYRAVIGNYSVIDSKEAALINRKELVE
                     GVKRVIALSSEEKIWATKFNFKGSVLNMTANSSFGGNSVDEILVDKPFTSDKSVGINA
                     RYLLDVLSILDKQEVNMIVEDGLKALTIKENTDNYQYIHLVMPLRI"
     gene            complement(2342..3679)
                     /gene="dnaA"
                     /locus_tag="PL056_09640"
     CDS             complement(2342..3679)
                     /gene="dnaA"
                     /locus_tag="PL056_09640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HPM47320.1"
                     /translation="MWKKIVTELKNTNSEGIINTFISPLFVIKESREEIIIGCPSEQI
                     LNVLEKNGWVDQIRALVTHLFNETVINFVIRSTDIDSDIQPSLPFIKEQAPVDREVRK
                     DVFLSVDFTFDNFVSGPSNQTAFAAAQGIAKNPGVHYNPFFIYGDSGLGKTHIIQAIG
                     NYILKNKNKSVYYTTFNQLMIDYVAALSSNTVAKFRSNILEHDVLLIDDIQFLSKKMG
                     TQIEFFNIFNSFYDAGKQIVLTSDRYISEIKDIDDRLRSRFVMGVILEIKPPEYETRL
                     AIVKKKSELYKINIDEETARLIASNLKNNVREIEGALRTLQISSELMGINRIYRSFAE
                     DILKDMLKKKGDLGIDEIIKLTSTCLHTKPSDIISSKRSKLISLSRQISMYLIKKYTS
                     KTLKEIGDSFGGRNHATVLSSISKIEQVILEDMNIKKIIDKLEREIEESKGSF"
     gene            complement(3777..4556)
                     /locus_tag="PL056_09645"
     CDS             complement(3777..4556)
                     /locus_tag="PL056_09645"
                     /inference="COORDINATES: protein motif:HMM:NF015559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HPM47321.1"
                     /translation="MTSLEKFSENLENDSVTVDRIYSRDIKICQHRNGYRFNSDSVIL
                     SWFIYRILGGKKINRSLEIGSGTGVVPIILKKRGFASAIECIEIQPGLFNLLQKNISD
                     NKLEDSLFPKNRDIREITKEKDVKYDLVFTNPPYFGVDNGKINPDNEKATSKHEFSGS
                     LSDFMSVSKKILNPGGHFIFIYPLSKIFFAMGSAMKNDFALNHIFLFRENPDLSPVSF
                     CAHLVFKGQSPVSVTDMITMRDDKGNYSKTGLEIMHEDQEG"
     gene            complement(4531..5160)
                     /locus_tag="PL056_09650"
     CDS             complement(4531..5160)
                     /locus_tag="PL056_09650"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:NF013468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HPM47322.1"
                     /translation="MEILSIEEAAWKIRNGLVVAYPTETVYGLGSIIFEKEPVMRIKQ
                     IKKIPENKPLSVLISNNKPDMIFELIDSFLPVAQRLAKFFWPGPLTILHECRADLPSF
                     ITGNTGFVGIRCSPFAFVNSFIDLVGHPVVSTSANPTGEKPAQNYMDVFSYFNNLIDG
                     VLICDDSISFEQKNIVFNVPSTIVKVDQHNFEIIREGAIKRDDIIRKIL"
     gene            complement(5162..5650)
                     /gene="purE"
                     /locus_tag="PL056_09655"
     CDS             complement(5162..5650)
                     /gene="purE"
                     /locus_tag="PL056_09655"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013658912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HPM47323.1"
                     /translation="MKKVMILMGSDSDWSIMKKAAEILKEFGVEFEVHVSSAHRTHSK
                     TIGLVEKFDGACFIIGAGAAAHLAGVVAAHTVKPVIGVPINATSIQGVDALYSTVQMP
                     SGIPVACMAVDGAKNAGLFALQIISVSDADIREKLINSRKAMVDEVNRKDEKLQTLVK
                     EI"
     gene            complement(5684..6169)
                     /locus_tag="PL056_09660"
     CDS             complement(5684..6169)
                     /locus_tag="PL056_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47324.1"
                     /translation="MKNIFIVFFTLFFFFYGFADQNSKDVLTLKYQAIAVGQISLAYR
                     LVALSTSLAIAKAVDPEYITGLLDNVDSTIANCKKIVSVNNDSPDAFNLEIFKSIDYL
                     LACSKNVKKYSSLQSYENLSSVRECIDESAKIVENLSERYNKLSSEKTKKTESPETVK
                     K"
BASE COUNT         1880 a         1161 c         1069 g         2217 t
ORIGIN      
        1 ttatgtggac tatttcatcg tgcccggaat ttttttcaac agaactggtc attatcacct
       61 gatttttcat ttctgtgatc attttaaaaa gttttttctt tttttcctca tcaagaaaga
      121 caaaaatgtc gtctatgaaa aaaacaggat aaatgttttt ttcttcatat atggcaattt
      181 cagaaagttt taaaatgaat gacagaagat atgtttcgcc cattgagagg ttgtttttta
      241 agcttccggt gtcaatatta atattcagcg ggtcaagatg cgggccgtaa agggaaaagc
      301 ccttatccat ctctttttcg agctttaaag aaagtttttc tgctgtgttt tcctcaatgt
      361 acgggtttaa tgcaatactg catcccggaa aaaatttttc tgacaatgac attatttttc
      421 cgtttattct gttacaaccg ttttttcttt tgtcccttat tttttctatc agttccacag
      481 cagcatgatt taaatattca accgctttga ctgaaccttt tttaagagcg gcgtttttct
      541 gctttataaa cctgtaatat tcaaccagct gatcaaaata actgctgtca tccatgaaac
      601 agatcctgtc aataaaatca cgccttccct tgggtcctcc gtttataagc tccatgtttt
      661 caggggcatg cataacagga aaaacaatgt tcatcagctc ttttcttgaa gagctaatcc
      721 cgtcaataag gatttttctt gagccatcat aattgtaacc tactgatata tctctgaaaa
      781 aagaattaaa gtcttccgta tgacatttta tgaaaaagtt atcttcccct ttttttatac
      841 attctttcat ttcatgagtt ttaaatgatt ttccacttaa aagaatataa ctggcttcaa
      901 taattgaagt ttttccagct ccgctttttc cgtttataat cattttgtca gaaaatgtca
      961 gatccagttt ttctaaattt ctgaaattta taagggcgat ctgtgaaatt ttcatgaacc
     1021 tgatactata ttcttaacgg cataacaagg tgaatatact gataattatc tgtgttttcc
     1081 ttgatggtga gtgcctttag accgtcttca actatcatgt ttacttcctg tttatccaga
     1141 attgaaagta catccagaag atatcttgcg tttattccga cacttttatc agatgtgaac
     1201 ggtttatcaa caagtatctc gtcaacagaa tttccaccga aacttgaatt tgctgtcata
     1261 ttaagaactg atcccttaaa attgaatttt gttgcccata tcttttcctc agaactcaaa
     1321 gctattactc ttttaacccc ttcaacaagt tcctttctgt ttataagtgc cgcttccttt
     1381 gagtcaataa ctgaataatt tcctatgaca gctctgtaat tcggaaattc atttttcaat
     1441 aatctggaaa taaaaagaac attttcactg cttataaata ttttatcatc atcgactgat
     1501 attttagctg tttcacaatc ggcaaatatc tttgagattt caaaaaagtt ctttccaggg
     1561 ataacaagac cgctttcaag tccatctatt tcagctgaaa aattctttat actgagaact
     1621 gaaagacggt gtatatcagt tgtcaccatt tcaatgaaat ttccatttgg atcactaact
     1681 tttgttataa gaacaccgga gtaagcttta tttacattgt gctcagtcat tgaaaactga
     1741 agttttttat aatatgagat gagcttgttt atttctatat cattgaattt cttgtcagtt
     1801 tcaggaattg caggaaaatc acctgaatct gaacacggaa cagtatattt tgttttttct
     1861 atagtaatat caagtatgtt gtttgctgta tcgtatttaa aaaggatatt atctcctctt
     1921 gccaccttgc agatgtcgtg aatgtatttt gcaggcacag ttattttccc gtcatcaaca
     1981 ttttcagaga taggaacttt cactacaact gtcgtttcaa ggtttgttgc atttaaagta
     2041 cattcttttc ctttgaactt gaaaagaata tgggaaagta tcggcattgt gttctttttt
     2101 tcaatggacg aactacaata gtcaagtgct ttaagcagct cttctttttt gaaagataat
     2161 ttaatcatta atttctcctt ttaaataaat acattattaa tagttgagca gtaatgtttt
     2221 aagtttaaaa atgaacaata aaaacaggta tatagatatg tctgaaaatg tttaatttta
     2281 tgttggataa atgtttttaa taaagctgac atttttataa cattcctttt ttcaacattt
     2341 gttaaaaact cccttttgac tcctcaattt ctctttctaa cttatcgatt attttcttga
     2401 tattcatatc ttcaagtatc acctgctcta tttttgaaat ggacgaaagg accgttgcat
     2461 gatttcttcc tccaaaactg tcgcctattt cctttaaggt ttttgaagtg tattttttta
     2521 tgagatacat gcttatctga cggctcagag atataagttt gcttcttttt gaagatatta
     2581 tatctgaagg ttttgtatga aggcatgttg aagtaagttt tattatttcg tcaatcccca
     2641 gatcaccttt cttttttaac atgtccttta aaatatcttc tgcaaatgac ctgtatattc
     2701 tatttattcc cattaattct gatgatatct gaagagttct gagtgctcct tctatttctc
     2761 tgacattatt tttaagattt gaagctataa gacgggctgt ttcttcatca atatttattt
     2821 tatagagttc agatttcttt ttaactatag ccagccgtgt ctcatattca ggtggcttta
     2881 tttcaagaat gacacccatt acaaaacggc ttcttaaacg gtcgtcaata tcttttattt
     2941 cggaaatata tctgtcagag gtaagaacta tctgttttcc ggcatcataa aaggaattga
     3001 atatattgaa aaattcgatt tgtgttccca ttttttttga caggaactgt atatcatcaa
     3061 taaggagcac atcgtgttca agaatgttgg acctgaattt tgcaacagta tttgaagaga
     3121 gagctgcgac ataatcgatc ataagctgat tgaaagttgt gtaatatact gatttatttt
     3181 tgttttttaa tatataattt cctatggcct gaatgatgtg tgttttaccc agaccggaat
     3241 caccatatat aaaaaaagga ttataatgaa cccctggatt ttttgcaatt ccctgtgccg
     3301 ccgcaaaagc agtctggttt gaaggccctg aaacaaagtt atcaaaagta aaatcaacgg
     3361 aaaggaaaac atcttttctg acttctctgt caacaggagc ttgttccttt atgaaaggaa
     3421 gagacggctg aatgtcgcta tcaatgtcag tagaccttat aacaaagttt atgactgttt
     3481 cgttgaaaag atgtgtcacg agggctctta tctgatcaac ccagccattc ttttcaagca
     3541 cattaagtat ctgttcgctt gggcagccga taattatttc ttcccgactc tcttttatga
     3601 caaaaagagg acttataaag gtgttgatga tcccttcact gtttgtgttt ttgagttcag
     3661 taactatttt tttccacatc cggcaaaacc cgctaagaaa tataaccaga acttttaaag
     3721 tctattgacc atattgtcct aatcattgat aagttttcaa gaaaaaaata ttttttttac
     3781 ccttcttgat cttcatgcat tatctccaac cctgtttttg aataatttcc tttgtcatca
     3841 cgcatagtta tcatgtcagt tacagagaca ggcgactgcc ctttaaaaac aaggtgggca
     3901 cagaaggaga caggagataa atcggggttt tctctgaaaa gaaaaatatg gttcagagca
     3961 aaatcatttt tcatagcgct ccccatggca aaaaagatct ttgaaagggg ataaatgaag
     4021 ataaaatgac ctcccggatt taatattttt tttgaaacag acataaaatc tgacagagat
     4081 cctgaaaact cgtgttttga tgttgccttc tcattatccg gatttatttt tccattatcc
     4141 acaccaaaat aaggggggtt ggtgaacacg agatcatatt taacatcctt ttcttttgta
     4201 atttccctga tatctctgtt tttagggaaa agagaatctt ctaatttgtt atcggaaata
     4261 tttttttgta aaagattgaa aagccccggc tggatctcta tgcattcgat cgctgacgca
     4321 aatcctcttt ttttgagtat tatcgggaca acacctgttc cggagccgat ctcaagagaa
     4381 cggttgatct tttttccacc aagtatgcgg tatatgaacc atgaaagaat aactgaatca
     4441 gaattgaatc tgtaaccgtt tctgtgttgg cagatcttga tatccctgct gtatattctg
     4501 tctacagtaa cggagtcgtt ttcaaggttt tcagagaatt tttctaatga tgtcatctct
     4561 ttttatagca ccctctctta tgatctcaaa attatgctga tctactttta ctatagtgga
     4621 aggaacattg aaaacgatgt ttttctgttc aaacgatatt gagtcatcac agatcagaac
     4681 gccatctatg aggttgttaa aataagaaaa cacatccatg tagttctgag ccggtttttc
     4741 tcccgtaggg ttggcgctgg tgcttacaac gggatgtccg acaagatcta taaaggaatt
     4801 tacaaaagca aagggcgagc atcttattcc cacaaaaccc gtgttgccgg taataaaaga
     4861 gggaaggtcg gctctgcatt cgtgtaaaat ggtcagagga cccggccaga aaaactttgc
     4921 caaccgctgt gcaacaggta gaaatgaatc aataagttca aatatcatgt cgggtttatt
     4981 gtttgagatc aggactgaaa gaggtttgtt ttcagggatt tttttaatct gtttgattct
     5041 cataaccggt tctttttcaa aaataataga cccgagcccg taaacagttt cggtcggata
     5101 cgctacaaca agtccgtttc ttattttcca agcagcttct tctattgaca gaatctccat
     5161 atcagatctc ctttacaagt gtttggagtt tttcatcttt tctgtttact tcatcaacca
     5221 ttgcttttct ggaattaatg agtttttccc tgatatcagc atcgcttacc gaaatgatct
     5281 gcaaagcaaa aagacctgca tttttagcac cgtcaacagc catgcatgca acgggaattc
     5341 ctgaaggcat ttgaactgtt gaataaagtg catccacacc ctgaatggag gttgcgttaa
     5401 tgggaacgcc tataaccggt tttacagtat gagcagcaac aactccggca agatgagccg
     5461 ccgctccggc accaataata aagcatgctc catcaaattt ttctacaaga ccgatggttt
     5521 ttgagtgagt tctgtgagct gaacttacat gcacctcaaa ctcaacgcca aactctttta
     5581 agatttctgc agcttttttc atgatcgacc agtcagaatc acttcccata agaatcataa
     5641 cttttttcat aaacactcct tatcttaatt atttaaataa aaatcacttt tttacggttt
     5701 ctggtgattc ggtttttttt gttttttcag aagacagctt gttatatctc tcgctgaggt
     5761 tttcaacaat tttggctgat tcatcaatgc actctctgac gcttgaaaga ttttcataac
     5821 tttgaagaga ggaatatttt ttaacatttt ttgaacaggc gagaagataa tcgatgcttt
     5881 taaatatttc aaggttgaaa gcatccggag agtcgttgtt gaccgaaact attttcttgc
     5941 agttggcgat agtggaatcc acattatcaa gaagccctgt gatgtattca ggatcaaccg
     6001 ccttggcaat agccagactg gtgcttaaag ccacaaggcg gtaggcaaga gatatctggc
     6061 cgacggcgat cgcctgatac ttaagcgtaa gaacatcttt gctgttctga tcagcaaacc
     6121 cgtaaaaaaa gaaaaaaaga gtaaaaaaaa caataaaaat atttttcatg acggcctcca
     6181 taaaaagctt ttacattata taaaaaaaga taatgttgtc aaaaatgata atatgccacg
     6241 aacctttttc tgtttccgag gtcatattct ctgacatggc ttaaaaactc gctttcaacc
     6301 ggtttctgac tgagcgaata ataaata
//
LOCUS       DAOOYB010000167         6312 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_14189_length_6312_cov_10.509989, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000167 DAOOYB010000000
VERSION     DAOOYB010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6312
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14189_length_6312_cov_10.509989"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..960
                     /locus_tag="PL056_09665"
     CDS             <1..960
                     /locus_tag="PL056_09665"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47325.1"
                     /translation="EEVYVKSKQSQMKGQDGQTEKEKKDEINDVGIHNLENGDKYIGG
                     FKNNRKHGEGEYIYATGERYKGMFENGVYNGYGEHILVDGTKYIGEFRNGLREGTGTL
                     FLTSGARYEGQFHDDLMHGEGVYRFSDEREYRGSFAKGIRQGKGVMLFPSGAEYEGDF
                     KNDKMTGKGKMRYKNENYYEGDFIDGKREGKGVLKKNNGDVYDGTFLANAMHGKGKYT
                     FANGDEYTGSIKMEKFTGEGVMKKTNGDVYKGTFLDGVYHGLGKLTGKNGSILYGMWH
                     YGKLARQLPVPENEEESTDDVAESENDINESDSGAEPSGFDEE"
     gene            989..4759
                     /locus_tag="PL056_09670"
     CDS             989..4759
                     /locus_tag="PL056_09670"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HPM47326.1"
                     /translation="MHRSFIWILFAVLSFSCATSGSSDLNEISIAIKSASSGDEIVPV
                     VNKAFSADYNLKGIESSVKELLVKYPENGELNEIAAFNAMLKSEKGKAFSHLIRSVLD
                     SENRNIPLMLELVFSMNLTANERKVFIRTLEILVSNSKDDNVKQLSRNILTHYYLLEG
                     IPEKAAFETDSNGFIRNWWIIGGFDNDNGKGFYDEYGPEKELDIKKTYKGKVVNVGWR
                     KIRFNGHKGFVPFDSFVSPSNDSLAYMLANIFSPVDKDVILNVSTSDAIKVWVNGSIV
                     FAEENISHFGYDNVRVKVKLFKGENTILFKTCQKSDVWSVGARITEMSEKESTDLVAL
                     HNFPKDSGKKTFESQIIFPYSEKEPVSEDLKETLYKGLILNFKGYEKYSMNYIRTAFE
                     KAPYNPFIMLYMAKALNVAGEEGKYIDLLNGAITHSSSELASFLIMRGRFFAKKGQKE
                     SAEEDYKKALESNPSSVEALKSQAGLFQSKGWHEDSRRILRKALEIEPDSPSLLKLLA
                     ASTNRLNYKEDAERIYRKALKLFPGDVSLNEELFHISREKRSSSSALYFLERIIDLSP
                     YNIRAYFDLFEFYRSSKDIDNALHQLEIIKDINPDNSKIHIKAGDLFYEQNKIPQALA
                     SWEKAHQLDPKNSYISERISYLKIEEKDESEKYVPDDDKIMEIIRNSIDFTADDGAET
                     LLVYDHAVCRINSDGSSKWYITEVSRALNDAGRDSLINSYLPYDGRKRILKTYSVNSN
                     FEKSEASSVSSFDIRFRQLKKGDFTVIQYIHYKPAPVFLENDFVGQWFVQSPMKHVIY
                     SEWNLIYPEGTKLNIDITGSRTEQVLEKLNDLTVHRFIARNIEPLNYEPNSPPLNNFL
                     ETVTVSTSESWDKYVNWERALLKDAFVSAKEIRDKAVKIVEGEQSVIGKINKIFEFVA
                     QDIRYQQEYENTIAGVKPHTAAQTLERGYGDCKDKAILFIQLAKELNIKADYVIIRTR
                     NAGTLQKTIPNQQFNHAIVYVPSQEGVESGFFMDPTVDLLEIGSLREDDQGATALKLD
                     IGTGKYEFIEIPYQSPELNFQKHDRVYNLSSEGILKVNDSISMRGGTSSYFRQTLRTK
                     DVGQKMFQRIANSLFKGGVLDKYEHSDIEDITAPFNISFSADVTNLVSVSSGKMTVSM
                     PEQIVNASIITLEKRKLPLWTGIPSLYEINVKFEIPEGFAVESMPNDISISNDCFSII
                     RTGTIIDENNVAIKSVFMKNCTEISVENYPQFRKLILEVIKKHNEYLVLVKK"
     gene            4769..5935
                     /locus_tag="PL056_09675"
     CDS             4769..5935
                     /locus_tag="PL056_09675"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HPM47327.1"
                     /translation="MKNKTITITLIILLFTLLSSLSGKEQTDAQTSATPNWNKDKKVI
                     ISEELKKTEKEKKIGWVAFPVVFYSSDTNFGFGGSAVLFKDIYKDEYINKSQSFAIVL
                     FYTLRNQLLNANVGNIYWDKANWHWGSKLIFSKYPTDFYGIGNHTDFKKGEVFEPFSF
                     QFENDIEYRFWRALYGGITLTQGYYMLLDSKNEGLADNYFENNRKEGFISGIGFKLNY
                     DSRNDSFFPTEGIKGETSFTYFPSGLGSQYSFYRYQFDIRGYIPFFFNSVIALQAVFD
                     AVEGDAPLSYLPSLGSKDLLRGYPGGRYRDNIYIAAQIEYRFPIWWRFGGVLFFGTGK
                     VQHEMMEFFFEDLKYAGGFGLRFQLNNKKRINFRFDMGFTPEGFNFYFNLLEAF"
     gene            complement(5945..6127)
                     /locus_tag="PL056_09680"
     CDS             complement(5945..6127)
                     /locus_tag="PL056_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47328.1"
                     /translation="MKLFVFAVSVTFLSTFYVTGADFCNGVTASGAFASAYEPPPSPP
                     NPDPPPPPPPPDFTKK"
BASE COUNT         2160 a         1033 c         1388 g         1731 t
ORIGIN      
        1 gaagaagttt atgtaaaaag caagcagtct cagatgaaag gacaggacgg gcagactgaa
       61 aaagagaaaa aagatgagat caatgatgtg ggcattcaca atctggaaaa tggcgacaag
      121 tatataggcg gttttaaaaa taacaggaag catggtgagg gagaatatat ttatgccaca
      181 ggggaaagat ataaaggaat gtttgaaaac ggtgtttata acggatacgg tgaacacatt
      241 cttgtagatg gaacaaaata tatcggagag ttcaggaatg gactgagaga agggacgggg
      301 acacttttcc ttacaagcgg agcaaggtat gaaggacagt tccacgatga tcttatgcat
      361 ggtgaaggtg tttacagatt ttctgatgaa cgggaataca gaggttcgtt tgcaaaaggg
      421 atcaggcagg gaaaaggggt gatgcttttc ccgagcggag ctgaatatga aggtgatttt
      481 aaaaatgaca agatgaccgg caaagggaaa atgagataca aaaatgaaaa ttattatgaa
      541 ggtgatttca ttgacggaaa aagagaggga aaaggtgtgc tgaagaagaa taacggtgat
      601 gtttatgacg ggacatttct tgcaaatgcc atgcacggaa aagggaaata tacctttgca
      661 aacggagatg aatacaccgg ctcaataaaa atggaaaagt ttaccggtga aggtgttatg
      721 aaaaagacaa acggtgatgt gtataaggga acatttctgg acggtgttta tcacggactt
      781 ggaaaactga ctggtaaaaa cggctccatc ctttacggaa tgtggcatta cggcaaactt
      841 gccagacagc ttccggtgcc tgaaaatgaa gaggaatcta ctgacgatgt ggctgaatca
      901 gagaatgaca ttaatgaaag tgattccgga gcagaacctt caggatttga tgaagaataa
      961 aaaagtttac ctatatatca gaggaaaaat gcacagatca tttatttgga ttttattcgc
     1021 agttttaagt ttttcctgcg caacatcagg aagctcggac cttaatgaaa taagcatagc
     1081 aatcaaatct gcgagcagtg gagatgagat agttcctgtt gtgaacaaag cattcagcgc
     1141 tgattacaat ttaaaaggaa ttgaatcatc tgttaaggaa ttgctggtga aatatcctga
     1201 aaatggagaa cttaacgaaa ttgcggcttt taacgcaatg ctcaaatctg aaaaaggaaa
     1261 agcattctca catcttataa gatcggtatt ggattcggaa aacaggaata ttccgctcat
     1321 gctggagctt gttttttcaa tgaatcttac tgccaatgaa agaaaggttt tcataagaac
     1381 tctcgaaatc ctcgtttcaa acagcaaaga tgacaatgta aaacaacttt caagaaatat
     1441 tctgacgcat tattatcttc ttgaaggaat tccagaaaaa gcggcattcg aaaccgacag
     1501 caacggtttt atcagaaact ggtggatcat aggcggtttt gacaatgata atgggaaagg
     1561 attctatgac gaatatggtc cggaaaaaga gctggatata aaaaaaacat ataagggaaa
     1621 agttgttaat gtcggctgga gaaagataag atttaacggg cacaagggct ttgttccttt
     1681 tgacagcttt gtttcgccat cgaatgattc tctggcatat atgcttgcga atattttttc
     1741 tcctgttgat aaagatgtga tcctgaatgt ttccacaagt gatgcaataa aggtgtgggt
     1801 taacggatca attgtgtttg cagaagagaa cataagtcac ttcggttatg ataatgtcag
     1861 ggttaaagtt aagcttttca aaggtgaaaa cacaatactt tttaaaacat gtcagaaaag
     1921 cgatgtgtgg tcggtaggcg ccagaataac tgaaatgtca gaaaaagaga gtactgattt
     1981 agttgcttta cataatttcc cgaaagattc cggaaaaaag acctttgagt cacaaattat
     2041 ttttccttat tccgaaaaag agccggtttc tgaagattta aaggaaacct tgtataaagg
     2101 attgatcctg aatttcaagg gatatgaaaa atattcaatg aattacatta gaacggcatt
     2161 tgagaaagct ccttacaacc ctttcataat gctgtatatg gcgaaagcac ttaatgtggc
     2221 tggagaggaa gggaagtata ttgatctttt aaacggtgcg atcacccatt cttcttcaga
     2281 actggcgtcg tttctaataa tgaggggacg gttctttgca aagaaaggcc aaaaggaaag
     2341 tgccgaggaa gattataaaa aagctcttga atcgaaccct tcatctgtcg aagctttaaa
     2401 aagccaggca ggcctttttc aatcaaaggg ctggcatgaa gattccagaa gaattctccg
     2461 gaaggcactt gaaatcgaac ctgacagccc cagcttgtta aagcttctgg cagcatcgac
     2521 gaatcgttta aattacaaag aagatgcaga aagaatatat agaaaagcac tcaaactttt
     2581 tccgggagat gtttctttaa acgaagaact ttttcacata tcaagagaaa aaaggtccag
     2641 ttcttcagcg ttgtatttcc ttgaaagaat tattgacctg tctccgtata acattcgcgc
     2701 atatttcgat ctttttgaat tttaccgttc atcaaaagac attgacaatg cattgcatca
     2761 gcttgagata attaaggata ttaaccctga taattccaag atacatatta aggcaggtga
     2821 tcttttctat gaacaaaata aaattccgca agctcttgca tcatgggaaa aggcacatca
     2881 gctcgatccg aagaacagct acatttccga aaggatatca tatttgaaga tagaggaaaa
     2941 ggatgaatct gaaaagtatg ttcctgatga tgacaaaata atggaaataa ttagaaattc
     3001 tattgatttt actgctgatg acggagcaga gacgcttctt gtttacgacc atgcggtgtg
     3061 cagaataaat agtgacggaa gcagtaagtg gtacattact gaagtgagca gggctctgaa
     3121 tgatgcaggc agagattccc tgataaactc atatctgccg tatgatggaa gaaaaaggat
     3181 cctgaaaact tattcagtca acagtaattt tgagaaaagt gaagcgagtt ctgtttcaag
     3241 ttttgacata aggttcagac agctcaaaaa aggagatttt acagttatcc agtatattca
     3301 ttataagccg gcgccggtgt tcctggaaaa tgattttgta ggacaatggt ttgttcaaag
     3361 tccaatgaaa catgtgatat actctgaatg gaatctcata tatcccgaag gaacgaagct
     3421 caacattgat attacaggca gcagaacgga acaggttctt gaaaaattga acgaccttac
     3481 agtacacaga tttatcgcca ggaacattga gccgctgaat tatgagccga attctccccc
     3541 gctgaataat tttcttgaaa ctgtgacggt aagcacctct gaaagctggg acaaatatgt
     3601 caactgggaa agggcgcttc tgaaggatgc cttcgtttca gcaaaagaga tcagagataa
     3661 agcggtaaaa atagttgagg gagaacaatc tgttatcgga aaaataaata aaatttttga
     3721 atttgttgca caggatatca gatatcaaca ggaatatgaa aatacaatcg caggggtaaa
     3781 accgcatacg gccgcacaaa cgcttgaaag aggatacggt gactgtaaag acaaggcgat
     3841 cctttttata caacttgcaa aagagcttaa cataaaagct gactatgtta ttattagaac
     3901 cagaaatgca ggaacactcc agaagaccat tccaaaccaa cagttcaatc acgcaatagt
     3961 ctatgttcct tctcaggaag gcgttgagag tgggtttttc atggacccga cagttgacct
     4021 gcttgagata ggaagtttaa gggaggatga tcagggggca acagcattaa aacttgacat
     4081 tggaacaggg aaatatgagt tcattgagat accttatcag tctccggaac ttaatttcca
     4141 gaaacacgac agggtttaca atctttcatc tgaaggcatc ctgaaggtta atgattcaat
     4201 atcaatgaga ggcggaacat cttcttattt caggcagact ctaaggacaa aggatgtcgg
     4261 acagaaaatg ttccagagaa tagcaaattc ccttttcaaa ggaggggtgc tagacaaata
     4321 tgaacatagc gatattgaag atataacagc gccgtttaat ataagttttt ctgcagatgt
     4381 tacgaatctt gtttctgttt cgagcggcaa gatgactgta agcatgcctg agcagatagt
     4441 aaatgcatca attataacac tggaaaaacg gaagcttcct ctctggacag gaatcccgag
     4501 tttatatgaa atcaatgtta aatttgaaat acccgaaggg tttgccgtgg aatcaatgcc
     4561 taacgacata tcaatatcaa atgactgttt ttcaattatc aggacaggca caattattga
     4621 tgaaaataat gtcgcaataa aaagtgtatt tatgaaaaac tgtactgaaa tatctgttga
     4681 aaactatccc caattcagaa agctcatact ggaagtcatt aaaaaacaca acgaatatct
     4741 tgtgttagta aaaaaataga gagggcgctt gaaaaataaa acaataacaa tcactttaat
     4801 catccttctt tttacattgt tatcatcttt atccggtaaa gagcaaactg atgcacagac
     4861 atctgcgact ccgaactgga ataaagacaa gaaagttatt atcagtgagg aattaaaaaa
     4921 aacggaaaaa gaaaagaaga tcggctgggt tgcttttcca gttgtatttt attcatctga
     4981 cactaacttc ggttttggcg gaagtgcggt gttgttcaaa gatatttaca aagatgagta
     5041 cataaataaa tcgcagtcat ttgcgatagt tcttttctat acgctcagaa accagctttt
     5101 aaatgccaat gtcggaaaca tttactggga caaagcaaat tggcactggg gaagcaaact
     5161 gatcttttca aaatatccga cagattttta tggaataggc aaccatacag atttcaaaaa
     5221 aggagaggta tttgagccat tctcattcca gtttgaaaat gacatagaat accgtttttg
     5281 gcgtgctctt tacggaggga ttactcttac gcagggatat tacatgcttc ttgattctaa
     5341 aaatgaaggg cttgcggaca actattttga gaacaacaga aaagagggat ttatttcagg
     5401 cattggattc aagcttaact acgacagcag aaatgattcg ttttttccga ctgaagggat
     5461 aaaaggagaa acaagcttta catattttcc atcagggctc ggaagtcaat attcatttta
     5521 ccgttatcag ttcgatatac ggggatacat tccttttttc tttaattccg ttatagcact
     5581 tcaagctgtt tttgatgcag ttgaaggtga cgctccatta tcgtatctgc cgtctcttgg
     5641 aagcaaggac cttcttcgcg gatatcccgg tggaagatat agggacaaca tttatattgc
     5701 cgcacagatc gaatataggt ttccgatatg gtggagattc ggtggagttt tgtttttcgg
     5761 cacagggaaa gttcagcatg aaatgatgga atttttcttt gaagacctta aatatgctgg
     5821 aggattcggt ttaagatttc agcttaacaa caaaaaacgg ataaacttca gatttgatat
     5881 gggctttaca ccagaagggt ttaatttcta ctttaatctt cttgaagctt tttagaaaaa
     5941 acagtcactt ttttgtgaaa tctggcggtg gcggtggcgg aggtggatca gggttcggag
     6001 gcgaaggcgg tggctcataa gcacttgcaa acgcacctga agccgtcaca ccgttacaaa
     6061 aatccgcacc agtaacataa aatgtactca aaaatgtgac tgaaactgca aaaacaaata
     6121 atttcatctc atgtctccaa cacaatacag ataaatttta atttatttgc tttcttaagt
     6181 caagtgtttg tcgaaaatta atttttgtaa tgaaattaaa tggttaatgc atacagatat
     6241 tccatggtta agttatttta agatcctgac acgcagattt tatcagggtc aagtgtattt
     6301 acataatctt tc
//
LOCUS       DAOOYB010000168         6168 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_14642_length_6168_cov_11.808114, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000168 DAOOYB010000000
VERSION     DAOOYB010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6168
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14642_length_6168_cov_11.808114"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..472)
                     /locus_tag="PL056_09685"
     CDS             complement(74..472)
                     /locus_tag="PL056_09685"
                     /inference="COORDINATES: protein motif:HMM:NF013844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HPM47329.1"
                     /translation="MSDKKKKDDFSKLLEEYSDIDFEIHKKEKSSTVEEQKRFFKPVE
                     TRPDHTLDLHGMLKDDALRKVEITVKDMKLRGYLKLRIITGKGKHSENEPVIPDAVDD
                     LLLKLKREGFIRTIAWENRIVKGSGFVDVS"
     gene            588..1685
                     /gene="mnmA"
                     /locus_tag="PL056_09690"
     CDS             588..1685
                     /gene="mnmA"
                     /locus_tag="PL056_09690"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HPM47330.1"
                     /translation="MSGRKKRVAVGLSGGVDSAVTAHILKEKGFDVCGITMSIWDGGI
                     TIDDLTKKGCYGPNKDKDIEDARKISEFLNIPFKVIDMKKEFQEHVVSYFKKEYLAGR
                     TPNPCVVCNRFLKFGAFLEKALSEGFDFDFFATGHYAKIVEENGEFFIKKAVYESKDQ
                     TYFLQGIKRELLKKILFPLGEMTKPQVREIARKIGLEVAERPESQDFIAGGDYSPLFA
                     NDNIKAGDIVDETGKVLGRHKGMIHYTVGQRKGLGISSEKPLYVLKIDPEKNRVVVTD
                     KENLFSDTLTAASFNPLVKFENINEFRALAKIRQKHKEAPATISFIDPNTLNIVFDEP
                     QLSITPGQAVAIYDGDKLLGGGIIEISAVLK"
     gene            complement(1838..2143)
                     /locus_tag="PL056_09695"
     CDS             complement(1838..2143)
                     /locus_tag="PL056_09695"
                     /inference="COORDINATES: protein motif:HMM:NF014548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRO family protein"
                     /protein_id="HPM47331.1"
                     /translation="MESKAVGVLKSIFDSLAKKINEKNIEFWFAREIMEHLGYVRWEN
                     FITAIDRAIQSCVSTGTDPDDHFRGVTKKVDLGSGATRNIDDFMLTRYACYCLKKGE"
     gene            2547..2912
                     /locus_tag="PL056_09700"
     CDS             2547..2912
                     /locus_tag="PL056_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47332.1"
                     /translation="MRTLILALTVMMMLLSCVEDEDINGNGDGKGWEKEWGSEARDRL
                     QAVSVGPDDTVYVGGRTFGDLYSDIEGERDATLAAFNPKGDEMWGRQWAVNDDENDVQ
                     TLIVDDEGNIYAGGGKVLL"
     gene            2912..3625
                     /locus_tag="PL056_09705"
     CDS             2912..3625
                     /locus_tag="PL056_09705"
                     /inference="COORDINATES: protein motif:HMM:NF018449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HPM47333.1"
                     /translation="MKFSPDGSKIWEKFPEVDSVSCLTLDKAGNVYAGVSSGDILKFS
                     PEGKELWHHNISTEDAKSQINALAVDSEGNIYAGGKTGEWDDLFGENAGGTDAFLVKI
                     APDKTHIWEKQWGGTGDDASRALVYDEIEDTIYVAGGRAFMIKKFSKDGKELWQLEGH
                     GVLSMTQDDQGNVFIGSGDVESLVEKYNLNGEKIWGSAVYKDAIAHAIACDSYGNVYA
                     AGVQNNNNFLIKISASEME"
     gene            3780..4949
                     /locus_tag="PL056_09710"
     CDS             3780..4949
                     /locus_tag="PL056_09710"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM47334.1"
                     /translation="MWIERDLVERLKKSVNSRPAVFVTGARQTGKSSLLKKEFPDAEY
                     VSFDRIIHAKQAEESPEYFLKSLEKQTILDEVQYVPEIFRELKVVIDNERSRYGKWLL
                     TGSQVFELSKNISESLAGRITLLHLETLSANEIRNSDAGELKNYLWKGGYPELWSNPD
                     LDHSEFFESYIKTYVERDLRSIVDVKNLSDFRKFFIILAARAGQLINYRDISKEVGVS
                     DVTIKNWLSALQICGLIYLLPPYYENIGKRLTKTPKLYFADHGLLCHLLNITTLESWH
                     SHIFKGALWENFVMMELIKSYGAIPGRNLFFYRDQNGVEIDFIVIGGDKLFLIEAKAA
                     EQHGSMKLNFSKVAPLFNKKYKVESIIAQNISESKVFRFRDFSAFNPLYCKNPDI"
     gene            5030..>6168
                     /locus_tag="PL056_09715"
     CDS             5030..>6168
                     /locus_tag="PL056_09715"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HPM47335.1"
                     /translation="MKQFFSMISIVVLVLFFFISCDNETKNTNDEEVSDQETGDEEAN
                     DDEASAIEMKYKKADEHAQAVSKEIVSANSALAMDLFSKLSFEESGVNMMISPLSISI
                     AMAMVTNGASDENLAEMKEVLGFNDMTMEAVNKQFYELIQSLVEADKDLVLSIANSVW
                     MDDQFEPGVKEVFLDALLESYEAEPFTIDFQADGAKDTINSWVSENTNGKIEKIIEEI
                     GADTVMYLINAIYFKAAWTVTFDKERTYEGDFQTTTAEQKKVQYMTFESDQEFEFYSS
                     GWEDGDYSVIRLPYGREKFAFYGIIPNGTGKTIDQFIFEMKDNGIEHYFKNLVKTEEV
                     PVILPKFKFEYEKSLVDVFKALGMEKAFAEGGFLNLADQGEGLYIS"
BASE COUNT         1958 a         1049 c         1351 g         1810 t
ORIGIN      
        1 taaaaataac gcatcattac aatattaaac tttaaatctc tattgtgaat ttaagatagt
       61 tttaagtcgt ttttcagctc acatcgacaa aaccggaacc ttttacgatt ctgttctccc
      121 atgcgatcgt tctgataaat ccttcccgct ttaatttaag tagaaggtcg tcaactgcat
      181 caggaatgac cggttcgttt tcagaatgtt tcccttttcc tgtaattatg cggagtttga
      241 gataccctcg caatttcatg tcttttactg ttatttcgac ttttctcagt gcgtcatctt
      301 tgagcattcc gtgaagatca agagtgtggt caggccttgt ctctacgggt ttgaaaaacc
      361 gtttctgctc ttcaactgtt gaactttttt cctttttatg gatctcaaaa tcaatatcgg
      421 aatattcttc aagaagcttt gaaaaatcat ctttcttttt tttatccgac attcttcctc
      481 cattcataac aaacttatca aaaataccat ttctgtcatc tttttcaagt tgattttact
      541 gtcaactgaa gtattctgtt tgagaatttg cttttccgga ggttgttttg tcgggcagaa
      601 aaaaaagagt ggctgtggga cttagcggcg gggttgattc tgctgtaact gctcacattc
      661 taaaggaaaa gggttttgat gtctgcggga taacgatgtc gatatgggac ggcgggataa
      721 cgatagatga tcttacaaaa aaagggtgtt acgggccgaa caaagataaa gatattgaag
      781 atgcccgcaa gatttcggaa ttcctgaata ttccgtttaa agtaatagat atgaaaaaag
      841 agtttcagga acatgtggta agctatttta aaaaggaata tcttgccggt cgtactccga
      901 atccgtgtgt tgtctgcaat agattcctta aattcggggc attccttgaa aaagctctgt
      961 ccgaggggtt tgattttgat ttttttgcaa cggggcacta tgcaaaaatt gttgaggaga
     1021 atggtgaatt ctttataaaa aaagcagttt atgaatcaaa ggaccagacc tattttcttc
     1081 aggggataaa aagggagctg ctgaaaaaga tactgtttcc gcttggagaa atgacaaagc
     1141 ctcaagttcg tgaaatagca cggaaaatag gacttgaagt tgcggaaaga cctgaaagcc
     1201 aggatttcat cgcaggtggc gattattcgc ctctttttgc aaatgacaac ataaaagcag
     1261 gtgacatcgt tgatgaaacg ggcaaagttc tcggtcgtca caaagggatg atccattaca
     1321 cagtaggtca gagaaaaggg ctcggcatca gcagtgaaaa accgctttat gttctgaaaa
     1381 ttgatcctga aaagaaccgt gtagttgtta cagacaagga aaatcttttt tcagacactc
     1441 ttactgccgc atctttcaac ccgcttgtca aatttgaaaa cattaatgaa ttcagagcat
     1501 tggctaagat aagacagaaa cacaaagaag cccctgcaac aatttcattc attgatccta
     1561 atactctaaa tatcgttttt gatgaaccgc agctttcaat aactcccgga caggcagtcg
     1621 caatttatga cggcgacaaa cttcttggcg gggggataat tgaaatctct gctgttttga
     1681 aataggacat cagtcatata agtgtgaaca ttcttgttac attaactgtc ttttgacttt
     1741 ggcagtctgc ccgatttttt tgcgagcttt ttttcttctg atttaaccca gcgctcaatt
     1801 tttctaatat cttcatcagg tggaagattt tccggtttta ttcccctttc ttcaagcaat
     1861 aacaggcata acgggtgagc ataaaatcat caatgtttct tgttgctccg cttccgagat
     1921 caaccttttt cgtgacgcca cgaaaatggt catcggggtc agttcctgtt gaaacacaag
     1981 attgaattgc acggtcaatt gcagtaatga aattttccca tcggacatat ccgagatgtt
     2041 ccattatttc tctggcaaac cagaattcga tatttttttc gttaatcttt ttagccagac
     2101 tgtcaaaaat cgattttaaa acaccgacgg ctttgctttc catattttcc tcctgccttt
     2161 gacacatgtc ctagtcagat taatatccta atgttttttg acaaacaact caaattatca
     2221 atttcgatca tctttcaacc ccagaaattt aatcagccaa tagataatgc aacaatcaga
     2281 ttagttttgc aagaaaatta ataagattat cttactcgta taaaaactgg aaaatgtgga
     2341 gtttatttaa ggacttggga tggatctgag gcagaaaaat atgtgcggta atagggattt
     2401 tgatcttttc tcaataggca gaggaataaa agtaatggga aatgaagtcg ccacattctg
     2461 acaagtggaa aaggacatat ctataatttg aaaaatcaca aatttatgat agcattatca
     2521 cgctgaacac ggagggaggg atattcatga gaacattaat tttggcatta acagttatga
     2581 tgatgttatt atcatgtgta gaagatgaag acattaacgg caacggtgat ggtaaaggct
     2641 gggaaaagga gtggggttct gaagcaaggg atagacttca ggcagtttct gtcggtcccg
     2701 atgacacggt ttatgtcggt ggaagaactt ttggggactt atattcagat atagaagggg
     2761 aaagagatgc aactcttgcg gcatttaacc ctaaaggtga tgaaatgtgg ggcagacagt
     2821 gggctgtcaa tgatgatgag aatgatgttc agacattgat tgtggatgat gaaggaaata
     2881 tatatgcagg ggggggcaaa gttcttttat aatgaaattc tctcctgacg ggtctaagat
     2941 ctgggagaaa tttcctgaag ttgacagtgt ctcctgttta acacttgata aagccgggaa
     3001 tgtttatgca ggtgtaagtt ccggagacat cttgaaattc tcccctgaag gtaaagagtt
     3061 gtggcatcat aatatttcaa cagaagatgc gaaaagtcag ataaacgctc ttgcagttga
     3121 ttcagaaggg aatatttatg cagggggcaa gacaggggaa tgggatgatc tgtttggaga
     3181 aaatgcaggg ggaacggatg catttttagt aaaaattgct cctgataaaa ctcatatctg
     3241 ggaaaaacag tggggaggga cgggggacga tgcctcccgg gctcttgtat atgatgaaat
     3301 tgaggacacc atctatgttg caggtggtag agctttcatg ataaaaaaat tctcaaaaga
     3361 tgggaaagaa ctatggcaat tagaaggaca cggagttctt tcaatgactc aagatgatca
     3421 agggaatgtt tttatcggta gtggagatgt tgaaagtctt gttgagaaat acaatttaaa
     3481 tggggagaag atatggggct ctgccgtata taaagatgcc attgctcatg caatagcctg
     3541 tgacagctac ggtaatgtat atgcggcagg agtacagaac aacaataatt tccttataaa
     3601 gatctctgca tctgagatgg agtgagtata tttttaagca tattggtgac ctgtgaatgc
     3661 agatctgcat gcattgaacg gcgtgaaatt tcaacaagtg ctaaaaaaac accttaattt
     3721 aatgcttgac tttaagattg ttcaaactta tactttgaat tatcttaaag gaggtgtcaa
     3781 tgtggattga aagagattta gtggaaagat taaaaaaatc tgttaattca agacctgccg
     3841 ttttcgtaac cggtgcaaga cagaccggaa agagttcgct tctgaaaaaa gagtttcctg
     3901 atgcggaata tgtctctttt gacaggatca ttcacgcaaa acaggcggag gaatctccgg
     3961 aatatttctt aaagagtctg gaaaaacaga caattcttga tgaagtccaa tatgttccgg
     4021 aaatattcag agaactcaag gttgttattg ataatgagag aagcagatac ggtaaatggc
     4081 tccttaccgg aagtcaggtt tttgaacttt caaaaaacat cagtgaaagt cttgcagggc
     4141 gaataaccct tcttcatctt gagacattga gtgcaaacga aataagaaat tcagatgccg
     4201 gagaactgaa aaattatctg tggaaaggcg gataccctga attatggagc aaccctgatc
     4261 tggatcattc tgaatttttt gaaagttata taaaaactta tgtcgaaaga gatctccggt
     4321 caattgtcga tgtaaaaaat cttagtgatt tcaggaaatt cttcataatt ttagccgcac
     4381 gggcaggcca gcttataaat tacagagata tatcaaaaga ggtcggagta agcgatgtaa
     4441 ctataaaaaa ctggctgtca gctctgcaga tctgcggact catttatctc cttcccccat
     4501 attatgagaa cattggaaaa agactgacaa aaactccgaa actctacttt gccgaccacg
     4561 gacttctgtg tcatctgctc aacataacaa cacttgaatc ctggcacagc catatattta
     4621 aaggagcttt atgggaaaat tttgtcatga tggagcttat aaaatcctat ggagcaattc
     4681 ccggcagaaa tcttttcttt tacagagatc agaatggagt tgaaatagat tttattgtca
     4741 ttggaggcga caaactgttt ctgattgagg caaaagccgc agaacaacat ggcagcatga
     4801 aacttaattt cagcaaggtg gctccgctat ttaataaaaa atataaagtc gaatcaataa
     4861 ttgcacagaa catttcggaa tcaaaagtgt tcagatttag agatttcagt gcattcaacc
     4921 ctctctactg caagaatcca gatatttgag gaaattaaat ttgaatgttt taagaaaatt
     4981 cgtagtttta tcacactaaa gcatcgtaaa cttttttatg ggggctgata tgaagcagtt
     5041 tttttcaatg atttcaattg ttgtgctggt tctgttcttt tttatttcat gtgataacga
     5101 aacaaagaac acaaacgatg aagaggtgag tgatcaggaa accggcgacg aagaagctaa
     5161 cgacgacgaa gcttcagcga tcgagatgaa atacaaaaaa gcggatgaac atgcgcaagc
     5221 ggtttcaaaa gagatcgttt ctgcaaattc agctcttgcg atggatcttt tcagcaaact
     5281 ttcatttgag gaaagcggtg tgaacatgat gatctctcct ctttcaattt caatagcgat
     5341 ggcgatggtg acaaatggtg caagtgatga aaatcttgcc gaaatgaaag aagttctcgg
     5401 tttcaacgat atgacaatgg aagcggtcaa taagcagttt tatgaattaa ttcagtcact
     5461 tgttgaagcc gataaagatc ttgtcctttc aattgcaaac tcagtctgga tggatgatca
     5521 gttcgagccg ggagtaaaag aagtattcct tgatgcactt cttgaaagct atgaagcaga
     5581 gccgttcacg atcgactttc aggcagatgg agcaaaagat acaataaatt catgggtttc
     5641 ggaaaataca aacggcaaga tcgaaaaaat aatcgaagag atcggtgccg acacagtaat
     5701 gtatcttatc aatgcgatat atttcaaagc ggcatggaca gtgacttttg ataaggaaag
     5761 aacttatgaa ggagatttcc agacaacaac tgctgaacag aaaaaagtgc agtacatgac
     5821 ttttgagagc gatcaggaat ttgaatttta cagctccggc tgggaagatg gagattacag
     5881 cgtcatacgg cttccctacg gcagagagaa gtttgcattc tacggcatta ttcccaacgg
     5941 aaccggtaaa acaatagacc agtttatatt tgaaatgaag gataacggca tagaacatta
     6001 ttttaaaaac cttgtgaaaa ctgaagaagt gcctgtaatt cttccaaaat ttaagtttga
     6061 gtatgaaaag tctcttgttg atgttttcaa agctctcggc atggaaaagg catttgctga
     6121 aggagggttt cttaatcttg ccgatcaggg tgaaggactt tacatttc
//
LOCUS       DAOOYB010000169         6161 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_14660_length_6161_cov_9.350311, whole genome shotgun sequence.
ACCESSION   DAOOYB010000169 DAOOYB010000000
VERSION     DAOOYB010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6161
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14660_length_6161_cov_9.350311"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1487
                     /locus_tag="PL056_09720"
     CDS             <1..1487
                     /locus_tag="PL056_09720"
                     /inference="COORDINATES: protein motif:HMM:NF017569.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPM47336.1"
                     /translation="KSNINLKNSESSNYASSLSQIILNEKMKNLSRNDSELSQKITED
                     VLDFINRTKKAFNKLDNPYEEEQLMLNSFKTISEKTFRAEWDKASSFVKTTYGNKELD
                     TVFYEKEFVKSLNPGIKDKKQAVQFESVKEHFIEKWEELLIKKQTKRELEFIDREREK
                     FCQELYKKIEELMKIQELLEPFTNELGRLWDMSKGSWNKNSFDIIKKYAELMKKDKAI
                     QDLAEMLGRMRSAEKEYEEELFTNIKIKPEWKFVHAEKSDLVGIRESDDLSALLPSET
                     VLLADKATETVFFKKFVEKKLQTFEYIAKQLSYSEEEFQDKRLREKEESKGPFIICVD
                     TSGSMHGTPENVAKTLCFALLKIALRDKRKCYLISFSTSIETLDLADLKNSIDKIIDF
                     LSMSFHGGTDATPAMKEALRMLSSDSYKKADVIVVSDFIMPEFDENSKDQIEKARETG
                     TKFHSLLIGRSQNTQVIKEFDNNWQYDPDRSESMITLLKNTNKL"
     gene            complement(1634..1708)
                     /locus_tag="PL056_09725"
     tRNA            complement(1634..1708)
                     /locus_tag="PL056_09725"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1673..1675),aa:Glu,seq:ctc)
     gene            complement(1724..1797)
                     /locus_tag="PL056_09730"
     tRNA            complement(1724..1797)
                     /locus_tag="PL056_09730"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1763..1765),aa:Gln,seq:ctg)
     gene            complement(1806..2165)
                     /locus_tag="PL056_09735"
     CDS             complement(1806..2165)
                     /locus_tag="PL056_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47337.1"
                     /translation="MFKNILLFDLKRVLCYKAVVFSVLLLLSYGLAANDLNLKYLPHQ
                     AAKVGDGKYQLMKDFDTSVKQVRALYVGDRYIKEDLLTNEENFRIHVFYNLKKSAPWH
                     KIYVISWDDKVFARVFK"
     gene            complement(2211..3209)
                     /locus_tag="PL056_09740"
     CDS             complement(2211..3209)
                     /locus_tag="PL056_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47338.1"
                     /translation="MKKFLFVFLALLIVFTGCKKEKQLQDQVDEFIQQYLYTVRDAVD
                     GTKGTIQEVYDNMLSKAMKEVVTFEDYKDHVTAIYKGRIGAELRTARAGIVIDKQTQE
                     FIEATGQTSNVGRMASAMNQDESLLFTVRVVKEGEVFKVEQQTLMAQITEQKEEQRRL
                     ENLLKDYKGLIKIDEIVGKKIPGRAGYAELIGTILNGSADLDMVKVGIRVRFKDKNGD
                     VIFAADFFPVVDMRFEGLRTSLLPNSVKVFKTVIKDIPEEWDPDQPLSFNFYLIDGKI
                     ISPEELAAENKERDKLKKLIEDTKKADEEARKQLKEIWEREKALRDKIKELQNQSN"
     gene            complement(3257..5188)
                     /locus_tag="PL056_09745"
     CDS             complement(3257..5188)
                     /locus_tag="PL056_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47339.1"
                     /translation="MNKFIKYAFVFFLFFSGCSKVEIIDTAEYSEESEPNGQSFSANE
                     ISAGKVYRAYISLAVNDSGDEDIFKFWSPAGTLISFEIESDENNFQPYIGHTDNLSHA
                     QFAIFNPPGKFRADFITSVDGWQYFEIGDIRNTVEEGERVGGFSYYFRMISQHICSFD
                     DNEKISSDEKIERSFPNGRSGVDILEMDIEENGIYQLKVETEEMFSDKFTFVLNCDAG
                     RVVAGNDDEDYYSNLMDPLIYSAFEKNLKNLIVTARMLVDLSSSGNENFTLSLVRQPE
                     NAELEPNNLYSSANIVTSDRMEGELSKEKRLILGELVEDQDWFRFDLLRGNVLDLSIK
                     AGNGGKFVSEIWAGSYSDTGTTIIPLRFNMLSGDETHRVNMLMPFSGNAYLILEGTDV
                     PYSIDVSVQDSVERILTFEGRVLETVDTPDCGWRFFEWTMPNMYDFFEISLESSEGKA
                     GMHIFDSRYRPHIFLEPNETNRFFVHRYENTGRLFFGMYFDECEQESDNTMELKILKR
                     KENFFEWDDSGRIFSIKYAGNGSYQGFFNTDELFVENLFDIEIEEDGTLFLQTGPSRD
                     TTAFDIDTVITLFKGYEKVDENDDAISFINYNKYSYLRHSVKKGEHYTVQVMPFMTES
                     SHVPSMNIIGNYILDIIIK"
     gene            complement(5181..5672)
                     /locus_tag="PL056_09750"
     CDS             complement(5181..5672)
                     /locus_tag="PL056_09750"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HPM47340.1"
                     /translation="MSEKTQKKDRIVTGIITVIILLLIGFNIFSYYGASIVLPGKAFP
                     LVSGVDPESGKAASIDFSKGTYIVDFWATWCGACVAEMDELNGISKKYPVYGVMKKPF
                     KKDVYSAISPQFGNVIVEDDLFNEYYISVIPTTILVKDGVISKVRTGGISAAMADEWF
                     KDE"
     gene            complement(5682..6089)
                     /locus_tag="PL056_09755"
     CDS             complement(5682..6089)
                     /locus_tag="PL056_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015255089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HPM47341.1"
                     /translation="MEKLNIKEVLENAKTIAIVGLSEKDGRTSNRIGKYLQINGKYRI
                     IPVNPNADEIFGEKTYKSLLDIPSEIKIDIVNVFRRSEFLEEVARDAVARGCGTFWAQ
                     LGVYDEKAAKILEEADIPFIMNRCIFVEHQNHL"
BASE COUNT         1893 a         1273 c         1113 g         1882 t
ORIGIN      
        1 gaaagagcaa tataaatcta aagaattccg aaagcagcaa ttatgcatct tcattatctc
       61 aaatcatttt aaatgagaaa atgaaaaatt taagcagaaa cgacagcgaa ttgtctcaaa
      121 agataacaga agatgttctg gatttcataa atagaacaaa aaaagctttc aacaaactgg
      181 ataacccata tgaagaagag cagttaatgc tgaattcatt taagaccata tctgaaaaaa
      241 ctttcagggc agaatgggac aaagcatctt cttttgtaaa gaccacttac ggtaataaag
      301 aattagacac ggtattttat gagaaagaat ttgtgaaaag tttaaatcct ggaatcaaag
      361 acaaaaaaca ggcagtacag tttgaaagtg tcaaagagca tttcattgaa aaatgggaag
      421 agcttttaat aaagaaacag acaaaaagag aacttgaatt tattgaccga gaaagagaga
      481 agttttgcca agagctttat aaaaagattg aagaacttat gaaaattcag gagttgctgg
      541 aaccgttcac caatgaactc ggaagactct gggatatgag caaagggagc tggaataaaa
      601 atagtttcga tattattaag aaatatgctg aattgatgaa aaaggacaaa gcaattcaag
      661 atcttgcaga aatgcttgga agaatgaggt cggctgaaaa agagtatgaa gaagagctgt
      721 ttacaaatat aaaaatcaag ccggaatgga aatttgttca cgcagaaaaa tcagatcttg
      781 tggggatcag agaaagtgac gatctgagtg cactgcttcc ttcagaaaca gtgcttcttg
      841 cagataaagc aacagaaacg gtatttttta agaaatttgt agaaaagaaa cttcaaacct
      901 ttgaatatat tgcaaaacaa ctttcataca gtgaagaaga atttcaggat aaaagactaa
      961 gagaaaaaga ggaatcaaag gggcctttca ttatatgtgt agataccagc ggttcaatgc
     1021 atggcactcc tgaaaatgtt gcaaaaacac tttgttttgc acttttaaaa attgctttac
     1081 gagacaaacg gaagtgttat ttaatttcat tttcgacaag catagaaaca cttgatctgg
     1141 cagacttgaa gaattccatt gataaaataa ttgattttct ttcaatgagt ttccatggcg
     1201 ggacagatgc aacaccggca atgaaagaag cattaagaat gttgtcttca gatagttata
     1261 aaaaagcaga tgtgatcgtg gtgtctgatt ttataatgcc ggaatttgat gaaaattcaa
     1321 aagatcaaat tgaaaaagcc agagaaaccg ggacaaaatt tcactcacta cttataggaa
     1381 gaagtcaaaa tactcaggtt attaaagagt ttgataataa ctggcagtat gatccggaca
     1441 gatcagaaag catgataaca ctcttaaaaa atacaaataa attataatct tggcgatcat
     1501 cctgatccgg aagttccatt caatttttct tatcaacatt gtcaatattt atggaaggta
     1561 ttttttgact aattcgattt ttttgatttt ttgcttactt gacttaactt cttgaaatca
     1621 ttcaggatta tcatggcgac cccaggggga ttcgaacccc cgttaacggc gtgagaggcc
     1681 gccgtcctaa ccactagacg atggggtcgg aataaataga ttttggatcc cggacaagga
     1741 ttcgaacctt gattgacgga gccagaaccc gtagtcctgc cattggacga tccgggatcc
     1801 tcatgctatt taaagactct tgcaaacact ttatcgtccc acgagatgac ataaatttta
     1861 tgccacgggg ccgacttttt taaattgtag aaaacatgta tccgaaaatt ctcttcattt
     1921 gtaagaaggt cttccttgat gtatctgtct cctacataaa gtgcccggac ctgcttaacc
     1981 gaagtatcaa aatctttcat caattgatat tttccgtcac ccacctttgc ggcctggtga
     2041 ggcagatact tcaagttcag gtcatttgct gcgagtccat agctcaaaag taaaagaacc
     2101 gaaaaaacga cggctttgta acacaaaacc cttttcaagt caaataaaag aatgttttta
     2161 aacatttttc accgatttat cttaataaaa tcataagctt gtattgtcaa ctagtttgac
     2221 tggttctgaa gttcttttat cttgtcgcga agagcttttt ctctttccca gatctcttta
     2281 agctgttttc ttgcttcttc atcagctttc tttgtgtctt ctatgagttt cttcagctta
     2341 tctctttcct tattttctgc tgcaagttct tccggagaaa tgatctttcc gtcgatgaga
     2401 tagaaattga atgaaagcgg ctgatcagga tcccattctt cgggaatgtc cttaataact
     2461 gtcttgaaaa cttttacgct gttgggaaga agtgaagtcc tgagaccttc aaatctcatg
     2521 tcgacaaccg ggaaaaaatc agcggcgaat attacatcgc cattcttatc tttgaatcta
     2581 acccttatgc ctactttcac catatcaaga tcagcacttc cgttaagaat ggttccgata
     2641 agttcagcat aaccggcacg acccggtatt ttctttccta ctatctcgtc tattttgatc
     2701 aaacccttat aatccttgag aagattttca agccgtctct gctcttcctt ctgctctgtg
     2761 atctgtgcca tcagtgtctg ctgttcaact ttgaatactt cgccttcctt gacaactctt
     2821 actgtgaaaa gaagtgactc atcctgattc attgcgcttg ccattcttcc gacatttgat
     2881 gtctgacctg tcgcttcaat gaactcctgt gtctgcttgt caataactat gccggctctc
     2941 gcagtcctca gttcagcacc gattctccct ttgtagatgg cagtgacatg gtctttgtaa
     3001 tcttcaaatg tcacaacttc cttcatagct tttgaaagca tgttgtcata aacttcctga
     3061 atggttccct ttgtgccgtc aacggcatcc cttacagtat aaagatactg ctgaatgaac
     3121 tcatcgacct gatcctgaag ctgtttttcc tttttgcagc cagtgaaaac gatcagtaaa
     3181 gccaaaaaaa caaataaaaa ctttttcatc agttcctcct gataattaaa agaaacactg
     3241 ttgcgttaaa atatattcac ttaataatta tgtcaagaat atagttcccg atgatgttca
     3301 tggacgggac atgtgaactt tcggtcataa acggcattac ctgcactgtg taatgttccc
     3361 cttttttcac gctgtgtcta agataactgt atttgttgta attgataaaa gagatcgcat
     3421 catcattctc atcaaccttt tcataacctt taaaaagagt aatgaccgtg tctatgtcaa
     3481 aagctgtcgt gtctctggat ggacctgtct gcagaaagag cgttccgtct tcttctattt
     3541 caatgtcaaa aagattctct acaaaaagtt cgtcggtatt aaaaaaaccc tgatacgatc
     3601 cgtttccagc gtatttaata gaaaaaatcc ttccgctgtc atcccattca aagaaatttt
     3661 cttttctttt tagtatcttc agttccatag tattgtcact ttcctgctca cattcatcaa
     3721 aatacattcc gaaaaaaagc cgcccggtat tttcatatct gtgaacaaag aacctgtttg
     3781 tctcatttgg ttcaagaaaa atatgcggtc tgtaccggga atcaaaaatg tgcattccgg
     3841 ctttaccttc agaagactcc agggatatct caaaaaaatc atacatatta ggcattgtcc
     3901 attcaaagaa acgccatccg caatcaggag tatcgactgt ttcgagcacc ctgccttcaa
     3961 atgtaaggat cctttcaacg ctgtcttgaa cagagacatc aattgaatat ggaacatctg
     4021 tcccttcaag gatcagatag gcgtttccgg aaaaaggcat gagcatgttt actctgtgtg
     4081 tctcgtcacc gctgagcatg ttaaatctga gaggaattat tgtcgttcca gtatccgaat
     4141 atgaaccggc ccatatttcc gaaacgaatt ttccgccatt acctgctttt attgatagat
     4201 caagtacatt ccctctcaaa agatcgaatc tgaaccagtc ctgatcttca accagctctc
     4261 cgagtatcaa tcttttttct ttgcttaact ccccttccat tctgtccgaa gtgacaatat
     4321 tcgcactgct gtacaagttg ttgggttcga gctccgcatt ctccggttga cggacaagag
     4381 acagtgtaaa gttctcattc cctgaagaac tcagatccac aagcattctg gctgtaacta
     4441 tcagattttt aagatttttc tcaaatgcag agtatataag cggatccatc agattgctgt
     4501 agtagtcttc atcatcgttt cctgccacga cccttcctgc gtcacagttt aaaacaaagg
     4561 tgaacttgtc agagaacatc tcttccgttt caactttaag ctgatatatt ccattttctt
     4621 caatatccat ctcaagaatg tccactccgc tgcgaccgtt ggggaaactt ctttcaattt
     4681 tttcatcaga acttattttt tcattatcat caaaactgca gatgtgttgc gagatcattc
     4741 tgaaataata ggaaaacccg ccaacccgct caccttcttc aacggtgttt ctaatgtcac
     4801 ctatctcaaa atactgccat ccgtcaaccg aggtaataaa atccgccctg aatttgcccg
     4861 gaggattgaa tattgcaaac tgtgcatgtg aaagattgtc tgtgtggccg atatagggct
     4921 gaaagttatt ttcatcgctc tcaatttcaa atgaaataag ggttccggca ggactccaga
     4981 atttgaaaat atcctcatct ccgctatcat ttacagccaa tgaaatataa gccctgtaaa
     5041 cttttccggc agatatttca ttggctgaaa agctctgtcc attgggttct gattcttcag
     5101 aatattccgc agtatctatt atttcaactt tggaacatcc ggaaaaaaac agaaaaaaga
     5161 caaatgcata ctttataaat ttattcatct ttgaaccatt catcggccat ggcggcactt
     5221 atcccgccgg ttctcacctt tgagatgacc ccgtctttga caaggatcgt tgtgggaata
     5281 accgagatgt aatactcatt gaaaagatca tcttcgacta taacattccc gaattgcgga
     5341 gaaattgcgc tgtaaacatc ctttttaaaa ggttttttca tcactccgta aaccggatat
     5401 ttttttgata tgccgttcaa ttcatccatt tcagcgacac atgccccgca ccaggtagcc
     5461 cagaaatcaa caatataagt cccttttgaa aagtctatcg atgccgcttt accgctctca
     5521 ggatcaacac ctgaaaccaa agggaatgct ttccccggaa ggactatcga agcaccgtaa
     5581 taggaaaaaa tattgaaccc gatcagaagt agaatgatca cagttatgat gccggtaact
     5641 atccggtctt ttttttgtgt tttttcagac atttaacctc attacaggtg gttctgatgt
     5701 tcaacgaaaa tgcatctgtt cataatgaac gggatatcgg cttcttcaag tatcttggcc
     5761 gctttttcat catagacgcc aagctgtgcc cagaaagttc cgcaacctct tgcaaccgca
     5821 tcacgagcaa cttcttcaag aaattcactc cttctgaaaa cattgactat gtctattttt
     5881 atttcagatg gaatgtccag aagagatttg tatgtctttt ctccaaatat ctcatcagca
     5941 ttgggattta caggtattat ccggtactta ccatttatct ggagatattt cccgatccgg
     6001 ttgcttgtgc ggccgtcctt ttcgctaagc cccactattg caatggtctt tgcattttca
     6061 agaacctctt tgatattgag tttttccatg gcatccccac tgaaaagaat cataacggat
     6121 tatgttatac gcaactttaa tggatttcaa ttaaaaagtg t
//
LOCUS       DAOOYB010000170         6147 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_14710_length_6147_cov_9.995732, whole genome shotgun sequence.
ACCESSION   DAOOYB010000170 DAOOYB010000000
VERSION     DAOOYB010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6147
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14710_length_6147_cov_9.995732"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..860)
                     /locus_tag="PL056_09760"
     CDS             complement(<1..860)
                     /locus_tag="PL056_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47342.1"
                     /translation="MKRSIFISILIIFASIPFFSSCSPEETHLDIECSSFNDCELGER
                     CYKAKCVSNLQEPKNPLDEKEEFGGIEYVTNKIFYQGRVSLPDGFVLPFQNLQVQYGK
                     KNFVQNVDKVNGTFWVRTNVVGTTLFILKTNYEDESKNVPLMLTVFPSNGENYFKRKH
                     IEFSVRETAVALVFLQPGIATTTNPLYNAALLEMIRGLDATQTLIRILTDKMTRVTPS
                     IIVSGDLDVQNAIAGAVNELFFGKPEKEKEEETPLALKDGITVTQDDKTDTFHHAVFR
                     DLPQEHSEEEV"
     gene            complement(866..1945)
                     /locus_tag="PL056_09765"
     CDS             complement(866..1945)
                     /locus_tag="PL056_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47343.1"
                     /translation="MKKKILIIFSLISGCSFLSGQSLTGLYALAENIAGYEDEDEEEI
                     EEEGEEEAENPAFENEDEFSAEKDEREEEDPFMKKEEKKVVDDFVPDDFKDEGVKKDV
                     FVQPVDDDFVVEKREKGKTHRYVEPDLARIVVPTYLYAENAFKSKKLFELYRNDEVII
                     LEESGDFMKVEFLGKEGWVPTQDVRIEKWHTFRISMELSGGVAGGGGEIKNFDVIGNY
                     VFRLNIAAVQDFVVGVEGRGLSFDTDVLYAGGGLMLRYYIHGVRSKKTRSALSAYAGY
                     LGGYEKKGDSYGLGIGESEYTVFNGVYAGGSLDYYFRTFEYMAIGLGGDFTFVKLYGE
                     TETAKLEKEFYQGGGHLSIMFNILR"
     gene            2143..3945
                     /locus_tag="PL056_09770"
     CDS             2143..3945
                     /locus_tag="PL056_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47344.1"
                     /translation="MKHFFFTTFFRHKTIAFLIYLFFFLILFLQLPLRKEISGNCDTW
                     LALSYSSYSLESIKSFLTGTEPGSPMHPVHNPLAYGESAPGIQLPIIIMKYLGLSDYW
                     VNYLYISAIFSLTAFAIFVFAGNFTVFFPAKMLAGFIFTVNNISFAHIDDSIIIFFFF
                     PVLALHFLFRWFHEKNIKFLVLSSVLAGIQIYFSFYVFFYQFVMIFIFSAVSVFKNRV
                     KLTELIKPAALYLLISAAIFVPHLLFYLKTIQELEFVEIFDKFYTVKMASINPLDFIL
                     VLPDNLIYPDLGKMTGIPMNWGFVRHYNFTGLIALALFIYSFFKWNNFRLFLGMTALA
                     GLFLAAGPVFMFNMKEVFYSPLYVFYKLIPALGFLRVAVRAHFIFLFAMSIGAALSTE
                     KLFKGKKYQNFFISAMFLLHFFESTPLPIKGFDASVTDKVPMIYNYIKKMDGRPLIIE
                     LPSRMDVEFLNWDDSIFNDPAEFIFKGTDRLSLKVENISMFVNSWDDIFQYNREIIYT
                     NWQTSHKIDSVNGVNGYFPVPRMMYQYHINRLPDKKSFQTLKKWGVNYIVWHESMKIE
                     GDTLSLEILNNSECLTRINTAEESSLFKLEECNE"
     gene            3938..4795
                     /locus_tag="PL056_09775"
     CDS             3938..4795
                     /locus_tag="PL056_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47345.1"
                     /translation="MNELFFIVYQIIISTRIFIEMEDLTLSVFIHQLFWFNSVLIFFF
                     ICFRYLLNIEKSRLFLLSAGSILTFIPLIYAKLSGTAWALNYIQPVSLKQVAFDLATL
                     LFFHEYNWPMFPELIALLSGSILLATYLSKNLKLSIITSVTSVYGSFLLLGFSWIAVN
                     PEHPTLFLIKSGFHDSQFYAFQLITVFSFFAIISLFKEVSDIFRNFGNPLFVLSFFFA
                     ITLILQLTYLKLFTVSTTLTDLSVTFLPAASIVMSILVLFLSIFRIRSFRIIFLPFWI
                     MAFSILNLF"
     gene            complement(4815..4902)
                     /locus_tag="PL056_09780"
     tRNA            complement(4815..4902)
                     /locus_tag="PL056_09780"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4866..4868),aa:Leu,seq:taa)
     gene            complement(4921..4994)
                     /locus_tag="PL056_09785"
     tRNA            complement(4921..4994)
                     /locus_tag="PL056_09785"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4960..4962),aa:Cys,seq:gca)
     gene            complement(5036..5110)
                     /locus_tag="PL056_09790"
     tRNA            complement(5036..5110)
                     /locus_tag="PL056_09790"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5076..5078),aa:Gly,seq:gcc)
     gene            complement(5164..>6147)
                     /locus_tag="PL056_09795"
     CDS             complement(5164..>6147)
                     /locus_tag="PL056_09795"
                     /inference="COORDINATES: protein motif:HMM:NF019271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding EGF-like domain-containing
                     protein"
                     /protein_id="HPM47346.1"
                     /translation="DGNRLKCNEIEGMGYVNDTEVLCTTFCDAWITTDCKCAAGFEKD
                     ADGLCVDIDECVKETDNCAAEGSECTNTEGSFTCTCAENYEGDGLVCTFCDQNDKCGA
                     GCSACSGDTLYCMDNGDSTSQCVECYQADQCDTVAGEFCTANHVCSLCPLPLTVATWD
                     SGDDGWTKDGAWVNKNSKMEWGSSTKYNSSYTHNLTYGTETVLTHCVNAGVSFTISLS
                     DDTSWESESGTDKNQRLYVECSGNGGTDWVSMTPPTLPSDQTKNSGCTTYYCDGNQSV
                     DRSFSAVAQTWMFPAGCIAPNALIRFRANGSSAWELYTPGWTVDTVEMKEF"
BASE COUNT         1645 a         1443 c         1053 g         2006 t
ORIGIN      
        1 acctcctctt cactgtgttc ctggggaaga tctctgaaaa ctgcatgatg gaatgtatcg
       61 gtcttgtcat cctgagtaac tgttatcccg tctttaagcg caagcggcgt ctcttcctct
      121 ttttcttttt cgggctttcc gaaaaaaagc tcattgactg ctcctgcaat tgcattctga
      181 acatcgagat cgcctgaaac aatgatactc ggtgtgaccc ttgtcatttt gtctgtaagt
      241 attctgataa gtgtctgtgt cgcatcaaga cctcttatca tctcaaggag tgccgcatta
      301 taaagcggat ttgtcgtagt tgctattccg ggctgaagga aaacaagcgc cacagcagtc
      361 tctctaacgc tgaactcgat atgctttctc ttgaaataat tctctccgtt tgaaggaaat
      421 accgtcagca tgagaggaac attctttgac tcatcctcat agtttgtctt aagtatgaaa
      481 agtgtcgttc ctacaacatt tgtgcggacc cagaaagttc cgttaacctt gtcgacattc
      541 tgaacaaaat tcttctttcc atactgaacc tgcagattct ggaaagggag gacaaatcca
      601 tccggaagac tgaccctgcc ctgatagaat atcttgttcg tcacatactc tattcctccg
      661 aactcctctt tttcatcaag cggatttttc ggttcctgaa ggttgctcac acattttgcc
      721 ttgtagcatc tttcgcccag ttcacagtca ttgaaagaac tgcattctat atcaaggtgc
      781 gtttcttcag ggctgcatga gctgaaaaaa ggaatggagg caaaaatgat caaaattgaa
      841 ataaagatcg atcttttcat atttctcacc tcaaaatgtt aaacatgatg ctcagatgac
      901 cgccaccttg atagaactct ttctctagct tggccgtttc agtttcaccg taaagcttca
      961 caaatgtaaa atccccgccc agacctattg ccatgtattc aaaagttctg aaataataat
     1021 caagtgagcc acctgcatag acaccgttaa aaactgtata ttctgattcg cctataccaa
     1081 gaccgtatga atcgcctttc ttttcatatc caccgagata accggcataa gcactgagcg
     1141 cacttcttgt ctttttgctc cttactccat gaatgtagta cctcagcata agaccgccgc
     1201 ccgcatagag aacatctgta tcaaaagaaa gacctctccc ctcgacaccc acaacaaaat
     1261 cctgaaccgc ggcgatattt aaacggaaaa cataattccc gatgacatca aaattcttta
     1321 tctcaccgcc accgccggca acaccgcctg aaagttccat cgagatcctg aaagtatgcc
     1381 atttttcgat ccgtacatcc tgagtcggga cccagccctc cttgcccaga aactcaactt
     1441 tcataaaatc tcctgattcc tcaagaatta tcacttcatc gtttctgtaa agttcaaaaa
     1501 gttttttgga tttgaatgca ttttcagcat aaagatatgt cggaacaact atccgggcaa
     1561 gatcgggctc aacatagcgg tgcgtcttcc ctttttctct cttttcaaca acaaaatcat
     1621 catcaaccgg ttgcacgaaa acatcttttt tgaccccttc gtccttaaaa tcatccggca
     1681 caaaatcatc aaccactttt ttttcttctt ttttcataaa cggatcttct tcttcccgtt
     1741 catccttttc agcagagaac tcgtcttcgt tttcgaaagc cggattttcc gcttcctctt
     1801 cgccttcctc ttctatctcc tcttcgtctt catcttcata gccggcaata ttttcggcta
     1861 aagcatacaa accggtcaaa ctctgaccag acagaaaaga gcagccagag ataagactga
     1921 aaataattaa gatttttttc ttcacaacaa acccctgata tctattttac cgacactaat
     1981 caactcttac ttcacaaact gaagcattta aattacataa tattactact tatagaattt
     2041 ttttgcaagc agatttcatg attccccgct cacaagctgc tctatcttga ctttgggtgt
     2101 taaaacagtt taatattcct gaattttttg agatttgtaa ttttgaaaca cttttttttc
     2161 acaacctttt tcagacacaa aactatcgct tttctcatct atctcttttt ctttcttatc
     2221 ctttttctgc aactacccct ccgcaaagag atctccggca actgtgacac atggcttgcc
     2281 ctttcgtatt cctcctattc tcttgaatcg ataaaatctt tcctgacagg cacagagccc
     2341 gggtctccga tgcatcctgt ccataatcca cttgcgtacg gagaatcagc tcccggaata
     2401 caactaccta ttattattat gaaatacctg gggctttcag attattgggt aaattatctt
     2461 tacatatccg ccatcttttc actgactgct tttgcaatat ttgtatttgc cggaaatttc
     2521 accgtttttt ttcctgcaaa aatgcttgca gggttcatat tcacagtaaa taacatctct
     2581 tttgcccaca ttgatgactc aataattatc tttttcttct ttcctgtgct tgcacttcat
     2641 tttcttttca ggtggttcca tgaaaaaaac attaaatttc ttgtactgtc atcagttctt
     2701 gccggaattc agatatattt ttcattttat gttttcttct atcagtttgt catgatattt
     2761 atattttctg ctgtatctgt tttcaagaac agagtgaaac tgactgaact tataaaaccc
     2821 gccgcactgt atcttttaat atctgccgct atctttgttc cgcatctgct gttttatcta
     2881 aaaaccattc aggaacttga atttgttgag atatttgaca aattctacac tgtaaagatg
     2941 gcaagtatca atcctttgga cttcatcctt gtacttcctg acaatcttat ttaccctgac
     3001 cttggaaaaa tgacagggat cccgatgaac tggggttttg tgaggcatta caatttcaca
     3061 ggattaattg cgcttgcact attcatctat tcatttttta aatggaataa cttccgtctt
     3121 tttcttggaa tgacagcact tgccggactt tttcttgccg caggtccggt cttcatgttc
     3181 aacatgaaag aggtcttcta ctcgcctctt tatgtttttt acaaattaat tccagcactt
     3241 ggatttctca gagtagctgt tagagctcac ttcatctttc tttttgcgat gtcgatcgga
     3301 gcagcccttt caactgaaaa actgttcaag ggaaaaaaat atcagaattt ttttataagc
     3361 gcgatgtttc tactccattt ttttgaaagc actcctcttc ccataaaagg ttttgatgct
     3421 tctgtgacag acaaagttcc aatgatctac aattacatta agaaaatgga tggcagacct
     3481 ctgataatag agcttccgag caggatggat gtcgaatttc tgaactggga tgattctatt
     3541 ttcaatgatc ccgctgaatt tattttcaag ggaacagacc gtctcagttt aaaagttgag
     3601 aatatttcaa tgttcgtaaa ttcatgggat gatatttttc agtacaacag agaaataatc
     3661 tatacaaact ggcagacttc ccataagatc gattcagtga acggtgtcaa cggatatttt
     3721 cctgttccga gaatgatgta ccaataccac ataaaccgtc ttcctgataa aaaatctttt
     3781 cagacgctga aaaaatgggg tgtcaattat attgtgtggc atgaatcaat gaaaattgaa
     3841 ggcgacacgc tctccctgga aatacttaac aattctgaat gcttaacaag aatcaacact
     3901 gctgaagaaa gttctctgtt caagttggag gaatgcaatg aatgagcttt ttttcattgt
     3961 ttaccagatc attatttcaa caagaatttt cattgagatg gaagatctga cgctttcggt
     4021 tttcattcac cagcttttct ggtttaattc agtattgata ttctttttca tctgtttcag
     4081 atatctgctg aatattgaaa aaagccgact cttcctactg tctgcaggtt caatactcac
     4141 ttttatccca ctaatctatg caaaactttc cggaactgca tgggcactca actatataca
     4201 gccggtatct ttaaaacagg ttgcctttga tcttgcaaca cttcttttct ttcatgaata
     4261 caactggccg atgtttcctg aactgatcgc actattatcc ggatcaatac ttttagccac
     4321 atatctttct aaaaacctga aactttccat catcacatcg gttacatctg tttacggttc
     4381 atttcttctg cttggatttt catggatagc tgtcaatcct gaacatccca ccctttttct
     4441 gattaaatca ggattccatg acagtcagtt ctatgctttt cagttaataa cggtcttttc
     4501 attcttcgca attatcagtt tattcaaaga agtatccgat atattcagaa attttggaaa
     4561 tccgttgttt gttctctctt ttttctttgc aatcactctg atcctgcagt tgacttatct
     4621 taagctgttt accgtatcaa ctactctgac cgatctttct gtaacatttc tgcctgcggc
     4681 atcaattgtc atgtcgatac ttgtcttatt tctttcaata ttcagaataa ggtctttcag
     4741 aataatcttt ctaccattct ggataatggc tttttcgatc ctcaatctgt tttaaaaagg
     4801 tttgtggaat ttcatggtgc cggagaccgg actcgaaccg gtacaggaga gtatcccgag
     4861 ggattttaag tcccttgcgt ctaccaattc cgccactccg gccggcaatc agagaataaa
     4921 tggaggcgcc ttccggattt gaaccggaga tggagatttt gcagacctct gccttaccgc
     4981 ttggctaagg cgcctctgaa actgagatat tcgattagtt aaagaccaaa aaaaatggag
     5041 cgggaaacgg gattcgaacc cgcgacgtca accttggcaa ggttgcactc taccactgag
     5101 ttattcccgc tcagtaaaag tggaatataa tgattagagc cgagttgtca aataattttt
     5161 tgattaaaac tctttcattt caactgtatc tactgtccag ccgggagtat acagttccca
     5221 tgccgatgaa ccgtttgccc tgaaacgaat caatgcattc ggtgcaatgc accctgctgg
     5281 gaacatccag gtttgtgcaa ctgctgaaaa agatctgtca actgactgat ttccgtcaca
     5341 gtaatatgtt gtgcatccgc tgttttttgt ctggtcactc ggtaatgttg gaggtgtcat
     5401 gctcacccag tccgttccac cgttccccga acattctaca tagagacgct gatttttatc
     5461 tgttcccgat tcgctctccc acgaagtatc atcactgagc gaaatagtga acgaaacccc
     5521 tgcattaaca cagtgggtca gaactgtttc agttccataa gtcagattgt gtgtatagct
     5581 gctgttgtat ttagtgcttg aaccccactc catttttgag tttttattta cccatgctcc
     5641 atcctttgtc cagccgtcat caccgctatc ccatgttgcg acagtcaaag gaagcgggca
     5701 aagactgcat acatggttag ctgtgcagaa ctctccggca actgtgtcac actgatctgc
     5761 ctgatagcat tcaacacact gtgatgtgct gtcaccgtta tccatgcagt aaagtgtgtc
     5821 acccgaacag gcggaacaac cggctccgca tttatcattc tgatcgcaga atgtacagac
     5881 aagtccatcg ccttcataat tttcagcaca tgtacaagtg aatgatcctt ctgtgtttgt
     5941 acattctgaa ccttcagcgg cacagttatc agtttccttc acacattcat caatgtcaac
     6001 acacaatcca tctgcatctt tttcaaatcc tgcagcacat ttgcagtctg ttgttatcca
     6061 cgcatcacag aaagttgtgc agagaacttc agtatcgttg acatatccca tcccttcaat
     6121 ttcattgcat ttcagccggt tgccgtc
//
LOCUS       DAOOYB010000171         6142 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_14725_length_6142_cov_5.745195, whole genome shotgun sequence.
ACCESSION   DAOOYB010000171 DAOOYB010000000
VERSION     DAOOYB010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6142
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14725_length_6142_cov_5.745195"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..831)
                     /locus_tag="PL056_09800"
     CDS             complement(76..831)
                     /locus_tag="PL056_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47347.1"
                     /translation="MENVYELLDYLPLEDIEVNDYVRPLINSAQVTYEKGEYQFSYFA
                     VHLIFMTNIYTTVWQVSQFYKERYNHSLLFARPYNGKEVKLDEISSVFEFSKLPEKDI
                     FEFFRLIGLDDSYVKSTKKLIDYRNEMAHATGKIQISSKDDFDDAVNGIISVMRNIHE
                     KFSITIKSFYEKELREYAKTELEEFFASPDEYVDFLFISNLHFSPNELLVCKEFGLNK
                     LKNRKKSGLSLEEVRRIVEFHTSINDKYLEKVG"
     gene            complement(831..3395)
                     /locus_tag="PL056_09805"
     CDS             complement(831..3395)
                     /locus_tag="PL056_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015216777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HPM47348.1"
                     /translation="MLDTQTKRRIDDCRDILVGKLPDPKAQIEQITIGLIYKFMDDMD
                     KETIELGGKAKFFSDYKVPDPEFPNDRNKDKVIEFSKYAWDKLMHPQVTAAEMLALYS
                     EAIVGMEKNPNIPQLFRDIFKNAYLPYRDPETLKLFLKTISEFEYTHSEKLGDAFEYL
                     LSVMGSQGDAGQFRTPRHIIDFLVSIVDPDKNDTILDPACGTAGFLISAYKHILLKYS
                     KDNKQKLNIYEYNKLEKKPFNNLGDKLTPDDCKKLVQNFAGYDISPDMVRLSLVNLYL
                     HGFNTPNINEYDTLSSEEKWNESFDVILANPPFMSPKGGIRPHKRFTISSNRSEVLFV
                     DYIAEHLNINGKAGIIVPEGIIFQSGTAYKDLRKMLVKNHLYAVVSLPAGVFNPYSGV
                     KTSILLMDKEVSKKREEILFIKIDNDGYNLGAQRNAVKGSQLEEAIKLAHKFVETGEF
                     EETPIAHLVPKAEIAKNGDFNLSGERYKAQILFNTDFPIVELKEVAEVISGQSPSSEF
                     YNEEGKGLPFYQGKTEFGEQFIGQPTKWTTSITKEAFENDILMSVRAPVGPVNYAIQH
                     ICIGRGLAAIRTNNSIKKEFLFYLLKSQEDKIKGNGGAVFDSISRNQIEAIQIPLPPL
                     SIQEEIVAEIEGCQKIIDGAKAVVANYKPKINIDPDWEMVELDSIADVISGNAFSSSD
                     FKDDNPIRCIKITNVGVREFVNDDTGRLPESFISQYSRFAVKSGDLVISLTRTIISSG
                     LKVAIVPESWDNTLVNQRVAGIKPTEKSNILFIYYYLCSDVVFRYVEDKSRSLMQPNL
                     SITDLKNLSIPCPSIDIQNEIVSQIEREQELVNANKVLIEIFEQKIKDRIAKVWGE"
     gene            complement(3408..5936)
                     /locus_tag="PL056_09810"
     CDS             complement(3408..5936)
                     /locus_tag="PL056_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HPM47349.1"
                     /translation="MKNEAHARIKINQLLMEAGWRFFDDEEGKANILLENHVKITQKE
                     IDAWGNDYEKTKNGSLDFLLVDSDNKPICVLEAKREKLHPLVAKEQARKYAKTVGARY
                     VILSNGIVHYFWDTTKGNPKPIYKFPSPEEIGAIKNWNPDRNSLANEEVAVDYIVKVQ
                     MPDYVQNPEWKGSIEESRDFIWSNKLRFLRDYQLNAILKLQKAVSEGKERFLFEMATG
                     TGKTLTAAAVIKLFLRTQNARRVLFLVDRLELEDQAWKAFTEYLKPDYLTYIFKEHKS
                     DWRKADIVVTTIQSLLHNDKYRYDFSPTDFDLLISDEAHRSISGNARAVFEYFHGYKL
                     GLTATPKDYLKGVDPDKVKENDPREIERRMLRDTYSTFGCEGGEPTFRYSLPDGVKDG
                     YLINPRVLDARTEITTQLLSDEGYSVLVQTKEGEETETFISRDFEKKFFSEKTNMILC
                     QTFLENALCDPITKEIGKTIIFAVSQNHARKLTEILNEMADQLFPGKYNSDFAVQVTS
                     QVADAQQMTINFTNNNLSGKTTWLEGYKSCKTRVCVTVGMMTTGYDCPDLLNICMMRP
                     IFSPADFVQIKGRGTRKNTFEYKLKNELNDDEIIRKDKEVFKLFDFFANCEYFEEKFN
                     YDEKLNLPKPKNEATGGGGEKGNILKYTNYMPDPIFSISEKQVGYEGMRVDRELFKKF
                     EDRIVMDDIIKKNVELGNWEHVVSHIQQEIFDKPEEYFNIEKLRKAAKVDRKISIREM
                     VEKIFGIIPKFKAKDELLEEEFDKFISICPPEEDVNLRALKYFFKAYVVDQDVRRIIM
                     DKDFAALYTNATLPISEYKKVSEKYREIIPTYVKDYVDIDRFAA"
BASE COUNT         1744 a         1250 c          975 g         2173 t
ORIGIN      
        1 tgtttccctt tttcttccag gattcgctat ggggaagttg ggggaggaat gccgacctga
       61 cctttaaggt tttctttatc caactttttc caagtattta tcattaattg atgtgtggaa
      121 ttcaactatt cgtcgaactt cttctaatga aagtcctgat ttttttctgt tttttaattt
      181 attcaaacca aattctttac aaactagcag ttcatttggc gaaaaatgca aattacttat
      241 aaaaagaaaa tcaacatatt catcaggtga agcgaaaaat tcttctaact ctgtttttgc
      301 atattctcgc aactcttttt cgtaaaaact ttttatagta atgctgaact tttcatgaat
      361 atttctcatt acacttatta tcccattaac agcatcatca aaatcatctt ttgaagaaat
      421 ttgtatttta cctgtcgcat gtgccatttc atttctgtaa tcaatcagct tttttgtact
      481 tttcacatag gaatcatcta aaccgattaa tctgaaaaat tcaaaaatgt ctttctctgg
      541 taatttacta aactcaaaaa cagacgatat ttcgtcaagt tttacttctt taccattgta
      601 tggtctggca aataacaagg agtgattata tcgctcttta taaaactgac tcacttgcca
      661 aaccgttgtg taaatatttg tcataaatat caaatgcaca gcaaaatatg agaactgata
      721 ttctcctttc tcataagtca cttgtgccga gttaatgagt ggacgaacat agtcgtttac
      781 ttctatgtct tccaatggca agtagtctaa tagttcatat acattttcca ttattcaccc
      841 caaactttag caattctatc cttaattttc tgctcaaaaa tttcaatcag taccttgttg
      901 gcatttacca attcttgctc tctttcaatt tgagaaacga tttcattttg aatatcaatt
      961 gaaggacatg ggatacttaa atttttcaaa tcagttattg ataaattggg ttgcataagt
     1021 gaccttgact tatcctctac atatctgaaa acaacatcag aacacaaata gtaatagata
     1081 aacaggatat tagatttttc tgttggcttt atacctgcaa cacgttggtt aactaaagta
     1141 ttatcccatg attctggaac gattgcaact ttcaaacctg aagaaataat tgtacgagtt
     1201 aatgaaataa ctaaatcacc actctttaca gcaaatcttg aatactgact tatgaaggat
     1261 tcaggaagtc tacctgtgtc atcatttaca aattccctta ctccaacatt ggttattttt
     1321 atacaacgaa ttggattgtc atctttaaaa tctgaactac taaatgcatt accacttatt
     1381 acatctgcaa ttgaatctaa ttccaccatc tcccaatccg gatcaatatt aattttcggt
     1441 ttatagttgg ctactacggc tttggctcca tctattattt tttggcagcc ctctatttca
     1501 gcaactattt cttcctgaat ggataatggg ggaagaggga tttggattgc ctcaatttga
     1561 tttctagaaa tactgtcgaa aacagcccca ccatttcctt ttatcttatc ttcttgagat
     1621 tttaacagat agaataaaaa ctcctttttg attgaattat ttgttcttat tgcagctaac
     1681 cctctaccta tacagatatg ttgaatagca taattaacag gtccaacagg agccctcacc
     1741 gacattaaaa tatcattttc aaacgcctcc tttgttattg aagtagtcca ttttgttggt
     1801 tgtccaataa attgttctcc gaattcagtt ttaccctgat aaaaaggcaa acccttccct
     1861 tcttcattat aaaattcact gctgggagat tgacccgata tcacctctgc cacttctttt
     1921 agctctacaa tgggaaaatc agtgttaaac aaaatttgag ctttatatct ctccccactc
     1981 aaattgaaat ccccgttttt cgctatttct gctttcggaa caagatgagc aatgggagtt
     2041 tcttcaaatt cgcctgtttc aacaaatttg tgggcaagtt ttatcgcttc ttccaactga
     2101 ctgcctttaa cagcatttct ctgtgcccca aggttatatc catcattgtc aatttttata
     2161 aaaaggatct cttctcgctt ctttgaaact tctttatcca tcaacagaat ggaagttttc
     2221 actccactgt atggattaaa cactcccgct ggcaaactta ctacagcata taagtggttt
     2281 tttacgagca ttttccgcaa atctttataa gcagttccac tttggaaaat aattccctcg
     2341 ggaacaatga tgccagcttt tccgttgatg ttgagatgtt cagctatata atccacaaac
     2401 agcacttcgc ttctgttgct gctgattgta aatcttttat gtgggcgaat tcctcctttc
     2461 ggactcataa atggaggatt agctaatata acatcaaagc tttcattcca cttttcttcg
     2521 ctgcttagtg tgtcgtattc gtttatattg ggtgtgttga aaccgtgaag atatagattc
     2581 accaaactta atcgcaccat gtcgggcgaa atgtcataac cggcaaaatt ttgcaccaac
     2641 tttttgcagt cgtcaggtgt gagcttatcg cccaaattat tgaaaggttt cttttccagt
     2701 ttgttatact cgtagatgtt caatttttgc ttattgtctt ttgaatattt gagcagaata
     2761 tgcttgtaag cagaaatcaa gaaaccggct gtaccacacg cagggtcaag aatggtgtcg
     2821 tttttatcgg ggtcaacaat agacacaaga aaatcaataa tgtgtcgtgg tgtcctgaac
     2881 tgacctgcat caccttgcga acccattaca gacaggagat attcaaaagc atcgccgagt
     2941 ttttcgctgt gtgtatattc aaattcactt atagttttaa gaaacagctt taaagtttca
     3001 ggatcacggt aaggaaggta tgcattttta aaaatatctc tgaaaagctg aggaatgttc
     3061 ggattttttt ccattcccac tatggcttca gaatagagtg ccagcatttc cgccgcggtc
     3121 acctgggggt gcattagttt gtcccatgca tatttgctga actcaatcac cttatctttg
     3181 tttctgtcat ttgggaattc cgggtcaggc actttgtaat cagaaaagaa ttttgctttt
     3241 ccacccaact caatggtttc cttatccata tcgtccataa atttataaat caaaccaatg
     3301 gtgatctgct caatttgggc tttagggtca ggcagttttc ctaccaatat atctctgcaa
     3361 tcatcaattc ttctttttgt ctgggtatct aacatttaat tctcctttta agcggcgaaa
     3421 cgatctatat caacatagtc cttaacataa gttggtatga tctctctgta tttctctgaa
     3481 acttttttat actctgatat cggtaaagta gcgtttgtat aaagtgcggc aaaatcctta
     3541 tccataatga tccttcttac atcctgatca acaacatagg ctttaaaaaa atatttcaaa
     3601 gctcttaaat taacatcttc ttccggtgga caaatagaaa tgaatttgtc gaattcttct
     3661 tcaagaagtt catcttttgc cttgaatttc ggaataattc caaaaatctt ttcaaccatt
     3721 tcccttattg aaatttttct atccactttt gcagctttcc gcaatttttc gatattgaaa
     3781 tactcttcag gtttatcaaa aatctcctgc tgaatgtgac tgaccacatg ttcccaattt
     3841 cccaattcca catttttctt tatgatgtcg tccattacaa ttcggtcttc aaacttttta
     3901 aaaagttctc tgtcaacacg cattccttca tagccgacct gtttttcact tattgaaaaa
     3961 atcggatcag gcatgtagtt tgtgtattta aggatatttc ccttctcacc accacctcca
     4021 gtcgcttcat ttttcggctt tggtaaattc agtttttcat cataattaaa tttttcttca
     4081 aaatattcac agttagcaaa gaaatcaaac aatttgaaaa cctctttgtc tttgcgaatt
     4141 atttcatcat catttaactc atttttcaat ttatactcaa aagtgttttt tcgtgttcct
     4201 cttcccttga tctgaacaaa gtcagcaggg ctgaaaatcg gtcgcatcat acaaatattt
     4261 aacaaatccg ggcaatcata tccagttgtc atcatgccga cagtaacaca aacccttgtt
     4321 ttacaagatt tataaccttc cagccaagtt gtttttccgc tcagattatt attcgtaaag
     4381 tttattgtca tttgttgagc atcagcaact tgagaagtaa cttggactgc gaaatcagaa
     4441 ttgtatttgc ccgggaaaag ctgatcggcc atttcattta atatttccgt caatttgcgg
     4501 gcgtgattct gacttacagc aaaaattata gtcttgccga tctcttttgt aatcgggtca
     4561 cacaaagcat tttccaaaaa agtctggcat aatatcatgt tggtcttttc agaaaagaat
     4621 ttcttttcaa aatcacggga aataaatgtt tctgtttctt caccttcttt ggtctgcact
     4681 aaaactgaat atccttcatc tgagagaagt tgggttgtta tttctgttct ggcatccaaa
     4741 accctgggat ttattaaata accgtctttc actccgtcag gcaaagaata tcggaatgtc
     4801 ggttctccac cttcgcaacc aaatgttgaa tatgtgtctc gcaacattct ccgttcaatt
     4861 tctcttgggt cgttttcttt caccttgtca ggatcaacgc ctttgagata gtcttttggg
     4921 gtggctgtca aacctaattt gtatccgtga aaatattcaa ataccgctct tgcattgccg
     4981 gaaattgagc gatgcgcttc atctgaaatt agaagatcaa agtctgtcgg ggaaaagtca
     5041 tagcggtact tatcattgtg cagtaaagac tggattgtag tgacaacaat atctgctttt
     5101 ctccagtcac ttttatgttc cttgaaaata tatgttaaat aatcgggctt caaatattct
     5161 gtaaaagctt tccatgcctg atcttccagt tccaatctgt caacaagaaa aagaactctg
     5221 cgggcatttt gcgttcttaa aaacagtttt attacagcag cagcagttaa tgtctttcct
     5281 gtacctgttg ccatttcaaa taaaaatctc tccttgcctt ctgaaacagc tttttgtaat
     5341 ttcaatatcg catttaactg ataatctctt aaaaaacgga gcttgtttga ccagataaaa
     5401 tccctgcttt cttcaatgct tcccttccat tcaggatttt gtacataatc aggcatttga
     5461 acttttacaa tgtagtcaac cgctacttct tcgtttgcaa gtgaatttct gtctggattc
     5521 cagtttttga ttgctccaat ttcttctgga gaaggaaatt tataaatagg ttttggattt
     5581 ccttttgttg tgtcccagaa gtagtgaaca ataccattag aaagaattac atatctcgca
     5641 ccgactgtct ttgcgtattt tctagcttgt tctttagcta caagagggtg aagtttctct
     5701 ctttttgctt cgaggacaca aataggttta ttgtcactgt caacgagcaa gaagtctaaa
     5761 gaaccattct tggttttctc ataatcattg ccccaagcat cgatttcttt ttgagttatt
     5821 ttcacatggt tttcaagaag aatgtttgct ttgccttcct catcatcaaa aaatctccac
     5881 cctgcttcca tcagcagttg gttgatttta attcttgcat gagcttcatt tttcataaat
     5941 tctcccccga cacaaatcag ggttgaaaca cggttcaacc cctacgaacc aattccgaca
     6001 ctcttgagtc caattcgaaa atattttaac aattgttatt ttataggcaa tttatttgta
     6061 acggctcccc tctatttttg aaagagaggg cggggggtga gttgtagggg cacgatttat
     6121 cgcgcccgtg taatggcgaa ag
//
LOCUS       DAOOYB010000172         6133 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_14750_length_6133_cov_11.917901, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000172 DAOOYB010000000
VERSION     DAOOYB010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6133
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14750_length_6133_cov_11.917901"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            36..1070
                     /locus_tag="PL056_09815"
     CDS             36..1070
                     /locus_tag="PL056_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011965933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HPM47350.1"
                     /translation="MSNKLNINQTEYSVILQQAVAEIRVARTLIAKQVNSTANSVYWN
                     LGKLLFEKQLEEGYGSGVVKQLSADLRSEFPDMGLSPRNLWDMKRFYERYHLADEKLR
                     RSVAVLPWRHNLLLLNKVKSFPAIEFYANEVATKGWSRDLLLNAIKMDSYSRAQTQVA
                     SNNFSQTLPAISADYANEVFKSSYNLGFLGITEPVKELELEKRLIAKIRNFILELGKG
                     FSFIGNQHRLEYNSKEYFVDMLFFHRGLRSLVAVELKIGSFKAEYIGKMNLYLSLLDK
                     LEKGENENQSIGIILCADKDHLDVEIALQDINKPIGVAEYQLLLPKDELQALLVEEIK
                     ASEEINEKQK"
     gene            1151..2767
                     /locus_tag="PL056_09820"
     CDS             1151..2767
                     /locus_tag="PL056_09820"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HPM47351.1"
                     /translation="MTTNEQTQLGNTLWKIADELRGAMNADDFRDYMLSFLFLRYLSD
                     NYENAAKKELGKDYPVLNGNGGKTPLSIWYEKNPDDVTEFEKQMRRKIHYVIEPQNLW
                     NNIAHLAKTQSNELLKTVQNGFKYIENQSFESTFQGLFSEINLDSEKLGRNYPERNKK
                     LCTIIQKIAEGISQFSTDKDILGDAYEYLIGQFAAGSGKKAGEFYTPQQISDILSAIV
                     TLDSHDPKKGEKKKIERVLDFACGSGSLLLNVRKKMMKANGTIGKIYGQEKNITTYNL
                     CRMNMLLHEVKDTEFEIHHGDTLLNDWDILNEINPAKKLEFDAVVANPPFSYRWDSSE
                     AMGEDFRFKSHGLAPKSAADFAFLLHGFHFLSDKGTMAIILPHGVLFRGGSEERIRTK
                     LLKDGHIDTVIGLPANLFFSTGIPVCILVLKKCKKPDDVLFINASSDENFEKGKRQNK
                     LLPEHINKIVETYQYRKEEPRYSRRVSMNEIEKNEFNLNISRYVSTSVDEELIDLKEV
                     NKKIVDIDKNITKARETHNKFLKELGLPPI"
     gene            complement(2776..3450)
                     /locus_tag="PL056_09825"
     CDS             complement(2776..3450)
                     /locus_tag="PL056_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008063137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPM47352.1"
                     /translation="METVISLRNIKKSYGDKVKTEVLHGIDLDINKGEFCALIGQSGS
                     GKTTLLNIMGLLDKPTSGTIVLFGKQIENASIDEIAAIRGRTIGFVFQFHHLIGAFNA
                     LENVMMPMMAATGGLATKAMKERARYLLDAVGMSDRLTYKPDEISGGQRQRVSIARAL
                     AMDPKIILADEPTGNLDTVMSNEVFSLLRKINSEQKTSFLIVTHDTKVASSCDRTVEI
                     VDGKLV"
     assembly_gap    3499..3598
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3599..4053)
                     /locus_tag="PL056_09830"
     CDS             complement(<3599..4053)
                     /locus_tag="PL056_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPM47353.1"
                     /translation="MFEIMVAVRYLIDEKKQTAFIILACAVGVAVMVFLYGLISGLQA
                     SLIDRTLGSQPHIVSNAHKEKPRSIVRSDDKTTVIGSESRGFDRPQYILDWKQKISSI
                     ENIDGIVAAAPHLRGAALVKRGDIARSVMMNGVDAESFGRIIPVKKKII"
     gene            complement(4053..5252)
                     /locus_tag="PL056_09835"
     CDS             complement(4053..5252)
                     /locus_tag="PL056_09835"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPM47354.1"
                     /translation="MKKVILIAVIIIAAGFFAAKILMPVKVPACVIGLKEFNQRLVVM
                     GRITPAAEVVLGSTISGRISSVKVEEGDKVEKGKVLLTIDDAELRSNLAIAEAAVNQA
                     KAKLDQISGTEVRIAEETLIERKHQLENATEKFERSRKLYENQSLSKSEFETAELALK
                     QAESQFEIASAREKSISKGGNDFNLMKASLAQAMANVQLSRDKLAGATVTAPFSGVIL
                     SKSVEQGGVVQPGSALFKLARTDEIFITTDIEEKNLSFVSEGQKAVASAEAYPDKKFD
                     AEVYYISKAVDPSRGTVRIKLRIPEPPDYLKTDMTVSVDMDVATVKDVILVPAAALDE
                     TDTPFVLTIENEKVTKRMVKTGARDSMNVLISEGLQKGDVIILPEDNDFPKIGKKVEH
                     AELTECR"
     gene            complement(5270..>6133)
                     /locus_tag="PL056_09840"
     CDS             complement(5270..>6133)
                     /locus_tag="PL056_09840"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HPM47355.1"
                     /translation="SEKLYTLSNISLLEEEPSFHITNAIHETLFKGTPYEHSVSGTAE
                     GIKNVTFDQIGQIREKYFSSNIFSVALSGAAGMDILEKTVAGFARNNVGKTNDFKLKN
                     VNLNDKVIKIPMKGRKMAHITRVFKAPSVYGSDFEKMKLLENYMTGQKSPMFQLLREK
                     QGLVYSFDVSGMSGVAGGYVVFSAITTPGNVKSVMNSIEKAIDHLRKGEIDRKYFLET
                     KNILKTSFANSVVKSNFHAYNLALEEALGLPFESYLKYTEVIDSIKENDITETALKWL
                     GDGFWVVAGEI"
BASE COUNT         1825 a         1277 c         1169 g         1762 t
ORIGIN      
        1 ttattgcagg gtttgtttcc aggagtaaat gagacatgag taacaaatta aacataaacc
       61 aaacagaata cagtgtaatt ttgcaacagg ctgttgcaga aatccgtgtt gcccgtacat
      121 tgattgcaaa gcaagtaaac agtactgcaa attccgttta ttggaatttg ggaaaattac
      181 tctttgagaa gcagttagaa gaaggttatg gcagtggtgt ggtaaaacaa ttatcggcag
      241 atttaagaag tgagtttccc gacatggggc tttcgcctcg taatttgtgg gatatgaaac
      301 gattttatga gcgttatcac ctggcagatg aaaaactgcg acggagcgtc gcagttttgc
      361 cttggagaca taatttattg ttgctcaaca aagtaaaatc attccctgcc attgagtttt
      421 atgcgaacga agtagctaca aaaggctgga gcagagattt gttgctcaat gccataaaaa
      481 tggacagcta cagtcgggcg caaacgcaag ttgcaagtaa caatttcagc caaacgctcc
      541 ccgccataag cgcagactat gccaacgaag tgtttaaaag ttcctacaat ttaggattct
      601 taggcattac tgaacccgtg aaagagttgg aactcgaaaa acgacttatt gccaaaatca
      661 gaaattttat tttagagttg ggcaaaggtt ttagttttat aggcaaccaa caccgtctgg
      721 aatacaacag caaagaatat tttgtagata tgcttttttt tcacagagga ttgcgctctt
      781 tggtagctgt cgaattaaaa ataggaagtt ttaaggccga atatatcggc aaaatgaatt
      841 tgtatctttc cctgctcgac aaattggaaa aaggtgaaaa cgaaaaccaa tccattggta
      901 ttatcctttg tgccgataaa gaccaccttg atgtggagat cgccttgcaa gacataaaca
      961 aacccatagg tgtagccgaa tatcaactgt tgctgcccaa agatgaattg caagccttgt
     1021 tggtagagga aataaaagcc agtgaagaaa ttaatgagaa acaaaaatga gtgtagatga
     1081 tgaaattttt tttaaaatat aaatttcaat tttctgaaat atttaacgaa caaaacaaaa
     1141 tggaaacaac atgacaacaa acgaacaaac tcaattaggt aatactcttt ggaaaatcgc
     1201 agacgaattg cgtggagcaa tgaacgcaga cgattttcgt gattatatgc tttcgttcct
     1261 gttcctgcgt tatctgagcg acaattatga aaatgcggca aaaaaagaat tgggtaagga
     1321 ttatcctgtt ttaaacggga atggcggtaa aacaccccta tccatttggt atgagaagaa
     1381 ccctgatgat gttactgaat ttgaaaagca aatgcgcaga aaaattcatt atgttattga
     1441 acctcaaaat ttatggaaca atattgcaca tctggcaaaa acccaaagca atgagttact
     1501 caaaaccgta caaaatggtt tcaaatatat agaaaaccaa tcgtttgaaa gcacttttca
     1561 gggattattt tctgaaataa atctggattc tgaaaaactc ggaagaaact accccgaaag
     1621 aaataaaaaa ctctgtacca taattcagaa aattgcagaa gggatcagcc agttttccac
     1681 tgacaaagac atactgggtg atgcttacga atacctgatc ggtcaatttg cagcaggctc
     1741 cggcaaaaaa gcgggagagt tttatacacc acaacaaatt tccgatattc tttctgccat
     1801 tgtaacactt gacagccatg acccaaaaaa aggcgaaaag aaaaagattg aacgggtgtt
     1861 ggactttgcc tgtggttcag gctcgttgct tctcaatgtc cgcaaaaaaa tgatgaaagc
     1921 aaacggaacc atcggaaaaa tatatggaca ggaaaaaaat ataactacct acaacctgtg
     1981 ccgaatgaat atgcttctgc atgaagttaa agacacagag tttgaaatcc accacggaga
     2041 cacattgctc aacgactggg atattttaaa cgaaataaat cctgctaaaa aattagagtt
     2101 tgatgccgtt gtagctaatc cacctttcag ctatcgttgg gattcatcgg aagcaatggg
     2161 tgaagatttt cgcttcaaaa gtcatggact tgctccaaaa tcagctgccg actttgcttt
     2221 cctgctgcat gggtttcatt tccttagcga taagggaaca atggcaatta ttctgcctca
     2281 cggtgtttta ttcagaggtg gttcagagga aagaattaga accaaacttt taaaggatgg
     2341 acatattgac accgttatag ggcttcctgc aaacttattc ttctcgacag gtatccctgt
     2401 ttgtatcttg gttttaaaga agtgcaaaaa gcctgatgat gtgttgttca tcaatgcaag
     2461 ttctgatgaa aactttgaaa aaggtaaaag gcaaaacaaa ttactgcctg aacacatcaa
     2521 taaaatagtc gagacttatc agtatcgcaa agaagaaccc cgttactccc gcagagtttc
     2581 aatgaacgaa atagaaaaaa acgaattcaa ccttaatatt tcccgatatg taagcacttc
     2641 agtggacgaa gagttaattg acttgaaaga agtaaacaaa aaaatcgttg atattgacaa
     2701 aaacattacc aaagcaagag aaacacataa taagttttta aaggaactcg ggcttccgcc
     2761 gatataaccc gctgatcaca ccagctttcc gtcaacaatt tcgactgttc tgtcgcagga
     2821 tgacgcaact ttggtgtcat gtgtgacaat cagaaaagat gtcttctgtt cagaatttat
     2881 tttgcgaagg agtgaaaata cttcgtttga cattacggta tcaaggttgc ctgtcggttc
     2941 atcggcaagt attattttcg ggtccattgc aagagccctt gctattgaaa cgcgctgtct
     3001 ctgtccgccc gatatttcat caggtttgta agtcagccgg tccgacattc ccactgcatc
     3061 cagcagatat cgggcccttt ctttcattgc ttttgtcgca agcccaccag ttgctgccat
     3121 catcggcatc atcacatttt caagtgcatt gaaagcaccg ataaggtggt gaaactggaa
     3181 aacaaaaccg atagtacggc ctcttattgc cgcgatctca tcaattgatg cattttcaat
     3241 ctgttttcca aaaagcacaa tggttccgga tgtcggttta tcaagaagac ccataatgtt
     3301 taaaagtgtc gttttccctg aaccggactg accgatgaga gcacagaact cccctttgtt
     3361 tatgtcaagg tctattccgt gaagcacttc ggtttttact ttatcaccgt atgatttctt
     3421 aatatttctc agtgaaatta cagtttccat aataaactcc taatgaatga ccgttgcagg
     3481 atcgactttt gacgcctgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntt
     3601 tataattttc ttcttgaccg gaatgatcct gccaaacgat tcggcatcta caccgttcat
     3661 cattacagag cgggcgatat cgcctctttt aacaagtgcc gcacccctga gatgcggagc
     3721 tgccgcaacg atcccgtcaa tattttcgat acttgatatt ttctgtttcc agtcaagaat
     3781 gtactgcggt ctgtcaaagc cgcgactttc agagccgatc actgttgtct tatcatctga
     3841 gcggactatt gatctgggtt tttccttgtg agcgtttgaa acaatatggg gctgagaacc
     3901 aagcgtcctg tcaataagag aggcctgaag tccgctgata agtccgtaaa gaaagaccat
     3961 tacagcaaca cccactgcac aggcaagaat tataaaagcc gtctgttttt tttcatcgat
     4021 cagatacctg actgcgacca ttatttcaaa cattatctgc actccgtaag ctctgcatgc
     4081 tcaacttttt tcccaatttt aggaaaatcg ttgtcttcag gcaggatgat cacatcacct
     4141 ttctgtaaac cttctgatat cagcacattc atgctgtccc ttgcccccgt cttcaccatt
     4201 ctttttgtca ccttttcatt ttcaattgtc agcacaaaag gggtgtccgt ttcatcaaga
     4261 gccgccgccg gaacaagtat cacatccttc acagttgcaa catccatgtc tactgaaacg
     4321 gtcatgtcgg tcttcagata atcaggcggc tcgggtatcc tcagctttat tctgaccgtg
     4381 ccgcgggaag gatccaccgc ttttgaaata taatagacct cagcatcaaa ctttttatcg
     4441 ggatatgcct cggctgatgc aactgctttc tgaccttcac tgacaaaaga tagatttttt
     4501 tcctcgatgt ctgttgttat aaatatctca tcagtccttg caagtttgaa aagagctgat
     4561 ccgggctgga caacgcctcc ctgttcaaca ctcttgctca gtatcacacc tgaaaatgga
     4621 gctgtcactg ttgcaccggc aagtttgtct ctggaaagct gcacatttgc catcgcctga
     4681 gccagagaag ctttcatgag gttgaaatca ttccctccct ttgaaatgct tttttctctt
     4741 gccgatgcaa tttcaaactg actttctgcc tgtttcaatg cgagctccgc tgtttcgaat
     4801 tcagacttcg aaagtgactg attttcatac agttttctgc ttctttcaaa tttttcagtc
     4861 gcattttcaa gctgatgttt tctttcgatc agggtttcct ctgcaatacg cacttccgtt
     4921 cccgatatct gatcaagttt tgcctttgcc tgattaacag ccgcttccgc aatggcaaga
     4981 ttggatctga gttcagcatc atcaatggta agaagaactt tccctttctc gactttgtca
     5041 ccctcttcaa ctttaacaga cgatatccgt cctgaaattg ttgaacccag aacaacttct
     5101 gcggcaggag tgattcttcc cattacaaca agacgctggt tgaattcctt taatcctatg
     5161 acacatgccg gaacttttac aggcattaat atttttgccg caaaaaaacc tgccgcaata
     5221 atgataacag cgatcagaat cacttttttc attcaaaaac tcctttagat caaatttccc
     5281 ctgcaacaac ccaaaatccg tcaccaagcc atttgagagc cgtttcagtt atatcatttt
     5341 ctttaattga atcaattact tcagtatatt tcaaataact ttcaaaaggg agtccgagtg
     5401 cttcctcaag agcaagatta tatgcatgaa aattactttt tacgaccgaa ttggcaaaag
     5461 aagtcttaag aatattcttt gtttcaagga aatatttcct gtcgatctcc cctttcctta
     5521 aatgatctat cgccttttca attgaattca taaccgattt cacatttccc ggagtggtta
     5581 ttgcagagaa aacaacatat ccgccggcaa ctcctgacat cccggaaaca tcgaaagaat
     5641 agacaagtcc ctgcttttcc cgaagaagct ggaacatggg cgatttctga ccggtcatat
     5701 aattttcaag cagcttcatt ttctcaaaat cactgccata gaccgatgga gctttaaaaa
     5761 cccttgtaat atgagccatt ttgcgaccct tcatcggaat ttttatcact ttatcgttga
     5821 gattaacatt tttcaacttg aaatcatttg tttttccgac attgttcctt gcaaaaccgg
     5881 caaccgtttt ttcaaggatg tccatccctg cagctccgga gagtgcaact gaaaaaatgt
     5941 tgctcgaaaa atatttttct cttatctggc cgatctggtc gaatgtaaca ttttttatcc
     6001 cttcagctgt tcccgacacc gaatgttcat aaggagttcc cttgaaaaga gtttcgtgta
     6061 tcgcgtttgt gatgtgaaaa gatggctctt cttcaagaag cgaaatattt gaaagggtgt
     6121 aaagtttttc gga
//
LOCUS       DAOOYB010000173         6124 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_14785_length_6124_cov_9.427748, whole genome shotgun sequence.
ACCESSION   DAOOYB010000173 DAOOYB010000000
VERSION     DAOOYB010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6124
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14785_length_6124_cov_9.427748"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            116..2116
                     /gene="tkt"
                     /locus_tag="PL056_09845"
     CDS             116..2116
                     /gene="tkt"
                     /locus_tag="PL056_09845"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HPM47356.1"
                     /translation="MSGFSELSNKSINSIRTLSIDAIQKANSGHPGLPMGAAPMAYVL
                     WKNHMNIDPANPLWDNRDRFILSAGHGSMLLYSLLHLSGFNVTMDDIKNFRQWGSKTP
                     GHPEYGMTSGVETTTGPLGQGIAYGAGMAIAERMLAARFNRPGFEIVDHYTFVLTGDG
                     CLMEGVSAEAASLAGHLKLGKLIYLYDSNDISLDGPTSMTFTEDVAKRYESYGFQVIT
                     VESGDTDLDAINNAIKDAKAEASKPSMIIIKTTIGFGSPNKSGKADSHGAPLGKDEVA
                     LVKKNFGVDPEKHFEIYDDVYTDFGTIAQRGKSARLSWESMFEKYCEEYPELGDLYKK
                     MKNLEISGHVEEFLPVFETGAKIATRSANGKVLGAMARNVPWLIGGDADLSCSTKTAI
                     GSEKWISADDFSGRNIHFGVREHAMGAIANGISYYGFFKPFTATFFSFVDYMRPALRL
                     ASLSKLETVFVFTHDSLAVGEDGPTHQPVEQLASIRCIPGLTTIRPADANETSVAWKY
                     ILENRNTPVALILTRQDIPVLDRLIYAPAAEALKGGYIISDCEDPEIVIIATGSEVHI
                     ALEAAEELKNKGKKIRVVNMFSVEIFEKQSTDYKEKVLPESLKKRLVVEAGSSFGWHK
                     YAKDSGIIISVDRFGESAPGNVVLEKFGFTSENIVNSILKHY"
     assembly_gap    2326..2425
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2426..3984
                     /locus_tag="PL056_09850"
     CDS             <2426..3984
                     /locus_tag="PL056_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47357.1"
                     /translation="CPDNKICKNGFCVDPEAGNTGDTGNSGNTGNTGDTGNTGNTGDT
                     GDSGDSGDSGDSGNSGDSGNSGDSGNSGDTGNSGDTGNTGNTGDTGDSGDSGNTGVEP
                     VCGNEAVETGEDCDLGFAKNGDPYCDYGLTEPCKVCDEYCKEKDGLNHYCGDGIIQRA
                     DCTGFENCVKTIGITDEACDPADNTKCLTDCSSLKLPKRIYVKSTAAGANNGTSWTDA
                     YADLQRALDDARRSIEEATAEEVEIWVAGGTYKPSFAAGKYSLRLRGTSGWYGAALRV
                     TIGGTIVHDGLTLASGTESGWYNFTVESGQSVDITYTATGTWPEHPWFEVRTDHDGVG
                     AAYFATLSGQATPQPFGNRHKSFSLVNNTKVYGGFLGTETDISERKLSGDAGHTSAHT
                     VIISGDNASYNVFRHNGNGVNSTAVLDGVTISGGNANPERVSYWLRYDGEGMISGIYQ
                     CSDYSGCWPSDDVHVAGPQWMYSRGAAMANYASDKNYPTLINVTVENCTTLNTGDDAT
                     YSGHVYSWDD"
     gene            4003..>6124
                     /locus_tag="PL056_09855"
     CDS             4003..>6124
                     /locus_tag="PL056_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding EGF-like domain-containing
                     protein"
                     /protein_id="HPM47358.1"
                     /translation="MSQKIILTGVLFLCAGFFSCTSGESNYCITAFDCDEGQICVDGS
                     CVEKTAGDTGNTGDSGNSGNSGDTGNTGNTGNTGDSGNSGDSGNSGNTGNTAPDPCDP
                     NPCIGIALSDETCTPSGENYSCGCVEGYYWNESVCEDIDECSDELLNKCSEFADCQNS
                     TGAYGCTCREHYSGDGFECIADTQQVSCENTPPENASWNISNADGVLTQTWDGTDWAP
                     KPDECVWDCALNYSKAGDICEPDTKTFTCPEKPGDISTVWNSVSSYSQTWSGTEWLPA
                     ETATEYNTTADTISCRYKCADYFSWNGTECAGETKIFTCPDKPAAGTVWNSVSSYTQV
                     WNGTSWLPVDSATEYNTATSTTSCRYICDTNYTWNSSICAADTRNNQECTGLPANAYW
                     NTATQISQTWNGTAWAPSTTGVHNTVESTTECRFRCNINYTWNGLICAADTKTFNCTA
                     KPGGDTTVWNTVSSYLQTWGGSSWTPPESDTVYDASASPTSCRYKCATYYNWNSANSQ
                     CEADTRIYVKANATGANNGTSWTNAFTDLQKALDRAKMITDSNPSIHMEIWVAGGTYK
                     PSFAAGNYSLRLRGTSGWYGAKLRVNIGGSIIHDNLTLSSGTASSWYNFTVASGQSVA
                     ITYTSAGNYPHEVWFEVRTGSNGAGAAYFATLAGQATPQPFGNRHKHFSLVNNTTVYG
                     GFNGTETELSQRKLPGDVGHTSAHT"
BASE COUNT         1871 a         1264 c         1390 g         1499 t
ORIGIN      
        1 cggcggatct gctttctttg aaaaaattaa gttctctttt ttgtttgcaa tagaaatttt
       61 ttttgtatat cctcgttgtc gaatagagtt atttttaaga aaaatggaga ataatatgtc
      121 aggattcagc gaactttcaa acaaaagtat caacagtatc agaaccttat caattgatgc
      181 catccagaag gcaaattcag ggcatcccgg acttccaatg ggagccgctc caatggccta
      241 tgttctgtgg aaaaatcaca tgaatataga tcctgcaaac cctttatggg ataaccgtga
      301 cagattcatc ctttcagccg gtcacggaag catgctgctc tattcccttc ttcaccttag
      361 cggttttaat gtaacaatgg atgatataaa aaatttcaga cagtggggaa gcaaaacccc
      421 ggggcatcct gaatacggaa tgacatccgg agttgaaacc acaacaggcc ctcttggtca
      481 gggcattgca tacggagccg gaatggctat cgctgaaaga atgcttgccg caagattcaa
      541 cagacccggt tttgaaattg ttgaccacta taccttcgta ctgacaggtg acggatgtct
      601 tatggaaggt gtttcggctg aagcagcatc attggcaggt catcttaaac tcggaaaact
      661 catttatctt tatgattcca acgacatatc tcttgacggc ccgacatcaa tgacattcac
      721 cgaagatgtt gcaaaaagat atgaaagtta cggttttcag gttataactg ttgaaagcgg
      781 agatactgat cttgatgcga tcaacaatgc aataaaagat gcaaaagctg aagcttccaa
      841 accttcgatg ataataataa aaactaccat cggattcggc tcccccaata aatcgggcaa
      901 agcagattcc cacggagcac cgcttggaaa agacgaagtt gcactagtaa agaaaaattt
      961 cggagttgac cctgaaaaac attttgaaat ctatgatgat gtttatacag atttcggaac
     1021 gatcgcacag agaggaaaat cggcccgttt gagctgggaa tccatgtttg aaaaatattg
     1081 tgaagaatat cctgaactcg gagatctcta caaaaagatg aaaaaccttg aaatatccgg
     1141 acatgttgaa gaatttcttc ctgtttttga aaccggtgca aaaattgcga caagaagtgc
     1201 aaacggaaaa gtgcttggcg ctatggccag aaatgttccg tggcttatag gcggagatgc
     1261 agaccttagc tgttcaacaa aaaccgcaat aggttcagaa aaatggattt ctgcagacga
     1321 tttttcagga aggaacattc atttcggtgt cagagaacac gcaatgggtg caattgcaaa
     1381 cggcattagt tattacggtt ttttcaagcc atttacggca acattcttct cttttgttga
     1441 ttacatgaga ccggctttac gacttgcgtc gctttcaaaa cttgaaaccg tttttgtttt
     1501 tactcacgat tctcttgcgg taggtgaaga cgggccgaca catcagccag tcgaacaact
     1561 tgcttcaata cggtgcattc ctgggctgac aacgataaga ccggccgatg caaatgaaac
     1621 atctgttgcc tggaaataca tacttgaaaa cagaaatacc cctgttgcgc tgattttaac
     1681 caggcaggac attccggttc ttgacagatt aatttacgct ccggcagcag aagctctcaa
     1741 agggggatat atcatttcag attgtgaaga tcccgaaatt gttataattg caaccggctc
     1801 tgaagttcat atcgctcttg aagccgctga agaacttaaa aataaaggga aaaagatcag
     1861 agttgtcaat atgttctctg tggaaatatt tgaaaagcag tcaactgatt ataaagaaaa
     1921 agttcttcct gaatctttga aaaaacggtt ggttgttgaa gccgggtcgt cattcggctg
     1981 gcacaagtac gcaaaagaca gtggaattat aataagtgtt gaccgtttcg gtgaatcagc
     2041 tccgggaaat gtagttcttg aaaagttcgg atttacatca gaaaatattg taaattcaat
     2101 acttaaacat tattaaaatt ctcagattga aaaaaattct gtaataagta taatactctc
     2161 cggcatatat ttaaagtcgg agtttgtcat gaaaaggatt ttattttttg tacttttgtt
     2221 ctgcatttca tgtagcagcg aaaatccata tgaatgcata accgcatttg actgtcctga
     2281 caataaaatt tgcaaaaacg gattctgtgt agatcccgaa gcaggnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnactgt cctgacaata aaatttgcaa aaacggattc
     2461 tgtgtagatc ccgaagcagg caataccggt gacacaggca actcaggaaa tacaggaaat
     2521 acaggtgata caggcaacac tggaaatact ggcgataccg gagactcagg cgactcaggc
     2581 gactcaggcg actcgggcaa ctcaggcgac tcaggcaact caggcgactc aggcaactca
     2641 ggtgatacag gcaactcagg tgatacaggc aacactggaa atactggcga taccggcgac
     2701 tcaggcgact caggaaatac cggggttgag ccggtttgcg gcaatgaagc tgttgaaacc
     2761 ggagaggatt gtgacctcgg ttttgcaaag aacggagatc cttactgcga ttacggtttg
     2821 actgaaccat gcaaggtctg tgatgaatac tgcaaggaaa aagacggact taatcattat
     2881 tgcggtgacg gaataattca gagggctgat tgcaccggtt ttgaaaactg cgtcaaaact
     2941 atcggcatca ctgatgaagc atgcgatccc gccgacaaca cgaaatgtct tacagactgc
     3001 agttctctta aacttccaaa aagaatatat gtaaaatcga cagccgcggg ggcaaacaac
     3061 ggaacaagtt ggacagatgc atatgccgac cttcaaagag ctctggatga tgcaagaaga
     3121 tcaattgaag aggcaactgc cgaagaagtt gagatctggg ttgccggcgg aacatataag
     3181 ccgtcttttg ccgccggaaa atattctttg cgtctacgcg gaacaagcgg ttggtatgga
     3241 gcggctttaa gagtcaccat cggcggaacg atcgttcatg acggactcac ccttgcaagc
     3301 ggtactgagt caggatggta taattttaca gttgaatcag ggcagtctgt tgatatcaca
     3361 tataccgcta ctggaacatg gcctgaacat ccctggtttg aagtcagaac tgatcatgac
     3421 ggtgtcggag cagcttactt tgcaacttta tcaggtcaag cgacacctca gccattcggg
     3481 aatagacaca aaagtttctc actggttaac aacacaaaag tttacggagg atttctcggc
     3541 acggaaacag atatttctga aagaaaacta tccggagatg caggacacac ttcagcacac
     3601 acagtcataa tcagcggaga taacgcatca tataatgttt tcagacacaa cggaaacggc
     3661 gtgaacagta cagctgtact tgacggagta acaatttcag ggggaaatgc aaatccggaa
     3721 agggttagtt actggctaag atacgacggg gaaggaatga tatccggcat ttaccagtgc
     3781 agtgattatt ccggttgctg gccttcagac gatgtccatg tcgcaggacc tcaatggatg
     3841 tattcaagag gtgccgcaat ggctaattat gcatctgaca agaactatcc gactcttata
     3901 aatgttactg ttgaaaactg cactacattg aataccggag acgatgcgac atatagcggg
     3961 catgtatatt catgggatga ctgataatat cgcgagggca aaatgtcaca aaaaataatt
     4021 ttaacaggag tccttttttt atgtgccggt tttttttcgt gtacatcagg agaaagcaac
     4081 tattgcatca ccgcttttga ctgcgatgaa gggcagatct gtgttgacgg atcctgcgtc
     4141 gaaaagaccg ctggtgacac aggcaatact ggtgattcag gaaattccgg caattcagga
     4201 gatactggta acactggtaa cactggaaat accggcgact caggaaactc cggcgattca
     4261 ggaaacagcg gaaacaccgg caacactgcg cctgatccat gtgatccgaa cccctgcata
     4321 ggtattgcat taagcgatga aacctgtaca ccgtcgggtg aaaattattc ctgcggatgc
     4381 gttgaaggtt actactggaa tgaatcagtg tgtgaagaca ttgatgaatg ttccgatgaa
     4441 ctgcttaata aatgcagcga atttgcagat tgccagaatt caacgggcgc ttatggatgc
     4501 acatgcaggg aacattactc aggggacgga tttgaatgca ttgcagatac tcagcaggta
     4561 agctgcgaaa acactccacc ggaaaacgct tcgtggaata tatcaaatgc agacggagtt
     4621 ctcacccaga catgggacgg aactgactgg gcaccgaaac ctgatgaatg tgtctgggac
     4681 tgtgctttga attattcaaa agcaggtgac atttgtgaac cggacacgaa aactttcaca
     4741 tgccctgaaa aaccgggaga catttcaact gtctggaatt ctgtttcaag ctactctcaa
     4801 acctggagtg gaacagagtg gcttcctgct gaaactgcaa cagaatacaa cacaacagcc
     4861 gatacaattt cctgcaggta taaatgtgct gactattttt cgtggaacgg aacagaatgt
     4921 gccggtgaaa caaagatctt cacatgccct gacaaacctg cggcaggcac tgtctggaat
     4981 tcagtttcaa gttatactca ggtatggaac ggcaccagct ggcttccagt ggactccgca
     5041 actgaataca acacagcaac aagcacaact tcatgcagat acatttgtga cacgaattat
     5101 acatggaaca gttcaatttg tgccgccgac acaagaaata atcaggaatg taccggtctt
     5161 ccggcaaatg cctactggaa taccgcaacg caaatttcac aaacctggaa cggaacggct
     5221 tgggctcctt caacaacggg tgttcacaac actgtcgaaa gcacaactga atgccgcttc
     5281 agatgtaaca ttaattatac ctggaacgga ttaatatgtg cggccgacac aaagaccttc
     5341 aactgtactg caaaaccggg aggagacact actgtctgga atactgtttc gagttatctt
     5401 cagacatggg gcggttcttc atggacacct cctgaaagcg acactgttta tgatgcttca
     5461 gcaagcccaa cttcatgcag atataaatgt gcaacatact acaattggaa ttctgccaac
     5521 tcacaatgtg aagctgacac aaggatttat gttaaggcaa atgcaaccgg ggcaaacaac
     5581 ggaacgagct ggacaaatgc attcacagat cttcaaaaag ctcttgacag ggcaaaaatg
     5641 atcaccgact caaacccttc aatacatatg gaaatatggg ttgccggcgg aacatacaag
     5701 ccatcatttg cggcaggaaa ttattcattg aggcttcgcg gaacaagtgg atggtatgga
     5761 gcaaaattaa gagttaacat cggcggctct atcattcatg acaatctgac tctttcaagc
     5821 ggaactgctt cttcatggta taattttact gttgcatcgg ggcaatctgt agcaataaca
     5881 tatacttccg cagggaacta tcctcatgaa gtgtggtttg aagtcagaac tggaagtaat
     5941 ggtgcaggtg cggcatattt tgcaactctt gcaggacagg caacacctca gccgtttggc
     6001 aacagacaca aacatttctc acttgttaac aacactactg tatatggcgg attcaacgga
     6061 actgaaacgg agctttcgca gcgtaaactc cccggagatg ttggacacac ttccgctcat
     6121 actg
//
LOCUS       DAOOYB010000174         6091 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_14901_length_6091_cov_10.841120, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000174 DAOOYB010000000
VERSION     DAOOYB010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6091
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14901_length_6091_cov_10.841120"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..1401)
                     /gene="rimO"
                     /locus_tag="PL056_09860"
     CDS             complement(103..1401)
                     /gene="rimO"
                     /locus_tag="PL056_09860"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HPM47359.1"
                     /translation="MKKLFFESLGCPKNRVDSEVMIAALMNSGWVITEDPGEADLIIL
                     NTCSFIADARDESVSRFFELHASLKKGARIGVAGCLPQLYPDLKNVLNEADLVIGIDN
                     IADISSIVEKGSSLKIKPSEFIYNSGMERVLTYSPYTCYVKIADGCENFCSYCSIPFI
                     RGKYRERGVPDIVSEVENLVANGVKEVVLISQDTTKFGFVNGSTLSMLLKEINSVVGD
                     FRIRVMYLYPGKVDRSLLETMKKLRKVVNYLEIPVQHISDSVLKGMNRTYSGNDIRKM
                     INEIKDVFGDNYALRTTFISSFPSEKKDDHELLKNFIEEGHFDYSGAFRYSKEEFTKA
                     GKMRSVPKKEADKRFAEIERAAHIAMEKRLDRFIGLEMDVLYEGIDPELNVPVGRCWH
                     QAPEIDGITILTNVENEKCGKYYKCRLVSREGVDFIGEIL"
     gene            complement(1414..2106)
                     /locus_tag="PL056_09865"
     CDS             complement(1414..2106)
                     /locus_tag="PL056_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47360.1"
                     /translation="MLKNRQLVTAVIVISVFFSGISASPLIIKGVDGTWLPVLASSWS
                     EKSGGVMLKPAEGVELVKLRKKLIDMFPDMTIEIINKNLFFPSTNIEALFSMLNNVDI
                     GIPVKTDSYSKETLDRPVRKSVDEFAAVKDELIEAVVENVVFEVEKGKLFLDVIITKR
                     TKKGDFSKLYGKHRIAVAFKMTNGIADAEDERNKYFGPLLFSKKGTKISFLPQSQETD
                     RTVIISRFMIGN"
     gene            complement(2093..2974)
                     /gene="lgt"
                     /locus_tag="PL056_09870"
     CDS             complement(2093..2974)
                     /gene="lgt"
                     /locus_tag="PL056_09870"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HPM47361.1"
                     /translation="MNTYFVWNADPVIAVLGTLQLRYYGIFFASTILIGFFLMRRILR
                     MYGYKDKMADDFLVWGVLGVVIGARLVHCFFYEPQFYLSNPLEILKIHKGGVASHGAT
                     IGLITAVLLFIRKYKIKFFELSDGIVFAAGVGATLVRLGNLFNSEIVGRETTASWGWK
                     FIRNSADSHIARNLATGKCDISSMDCLIQYWPVRHPSQLYEAAGGLTILLIMLAVSFV
                     FKDLKKPGIYVGIFLTGYFTFRFFVEFFKEHQVFMDGTLDMGQYLSFPFIIFGLFAFY
                     YAFRIYKDDPGVKNVKK"
     gene            complement(2994..>3635)
                     /locus_tag="PL056_09875"
     CDS             complement(2994..>3635)
                     /locus_tag="PL056_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47362.1"
                     /translation="FCKMDEKEKKEQIDHCKLNDLTTDERKSCTHVMVRHENTSISIM
                     LAEVEELKDYKKFLDKIYSATLNDITFTILEKPDVPVENTSFVLEAELFAQPVSSFKA
                     DGTKCDSSSDEGCVSKGVDFEGNIENYFSDDDKIKERYLVGVFGTSTFGDSEKLELLY
                     TYEGKDILQKSIKHLDFQIGAKSFYLFFPGAQRPTGKLTADIKAKLLFNVEPL"
     assembly_gap    3636..3735
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3736..4079)
                     /locus_tag="PL056_09880"
     CDS             complement(<3736..4079)
                     /locus_tag="PL056_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47363.1"
                     /translation="MKSKAISKIVIFLTAFLILSSCSEFLLIPITVDYPFDKDSPSSP
                     SEYEIDIDEGLGLLKGLMAKDVSKTILENQLKSQYPGADVTVSVTSSEMSLDNILELI
                     TGVTVSRKIDYTA"
     gene            complement(4069..5190)
                     /gene="prfB"
                     /locus_tag="PL056_09885"
     CDS             complement(4069..5190)
                     /gene="prfB"
                     /locus_tag="PL056_09885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HPM47364.1"
                     /translation="MALNLRELEQITNDIAGRAGRLSESLKIDSKRNRLKELDNLLNS
                     PEIWNDSLKMAALNKEKRAIQNSIVLFDQFESKFDELKIARELFEEDSSMEPEAQKVL
                     DETNSILDRIEFQKMLGGKTDILNAIVSINAGAGGRESQDWAVMLFRMYTRWCERKGY
                     KLEIIDKVTGDDAAGIKNVTLIAEGDYAHGYLKAEIGVHRLVRISPYDANKRRHTSFA
                     SVFVTPEIDDSIEVEIDEKDLKVDTFRSGGAGGQHVNTTDSAIRITHLPTGIVTQCQN
                     ERSQHKNRATAMKILRSKLYEFEMKKKMEAISENDKQKMGIDFGSQIRSYVLHPYKMV
                     KDHRTDFETGNAEAVLDGEEILDKFMEDYLLKTGKNNEK"
     gene            complement(5194..6024)
                     /locus_tag="PL056_09890"
     CDS             complement(5194..6024)
                     /locus_tag="PL056_09890"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPM47365.1"
                     /translation="MKKGLFLIFAVLLAVSCGSFKDEGIIYVFKGEAVSVSDFKAKYV
                     LWLRQNGLTDSDELRRNYLFNELSEKLLYETGLKEGVESIPEVKIKIDNFRKNTIIEY
                     MKKRAKKEIYAIDDEAVRAYYIENKDKFIRDKLYRLYAVRVSSKKMADDIVEQLRKGS
                     SIRMLSARFSNDESLSRNNGDWGLFSEEVMDELWKKDVLSGLPGEILGPYLDSDNYYT
                     IIEIAGFAYKRHLSFNLAYPLIIEELVGQSGTEKWDRYTDMMIKEYGAKINMENLNWE
                     "
BASE COUNT         1678 a         1361 c         1035 g         1917 t
ORIGIN      
        1 ggaggttttt gtcaagtttt ttattgaaaa tcgaacgatt taggttttag tcttttaatg
       61 ttttcaatgt ttctattaca aatagatgtc atgtgtggca aactatagaa tttcgccaat
      121 gaaatctacg ccttcgcggg aaacaagccg gcatttataa tatttgccgc atttttcatt
      181 ttcgacattc gtgagtattg tgataccgtc aatctcaggt gcctgatgcc agcatctgcc
      241 gacgggaaca tttaattccg gatcgatccc ttcgtaaaga acatccattt caagcccgat
      301 aaacctgtca agccgttttt ccattgcaat atgagccgct ctttcaattt ctgcgaacct
      361 tttatctgcc tcttttttag ggacagatct cattttccct gctttcgtga actcctcttt
      421 tgaatatctg aatgcaccgg aataatcaaa atgaccttct tcaatgaagt ttttaagcag
      481 ttcgtggtca tctttttttt cagacggaaa tgaggaaatg aaagtggttc tcagtgcata
      541 gttatcaccg aaaacatctt ttatttcatt gatcattttt ctgatatcgt ttccgctgta
      601 ggtgcgattc atccctttaa gcacactatc gcttatgtgc tgaacaggaa tttcaagata
      661 attgaccact tttctgagtt ttttcatcgt ttcaagaaga gatctgtcaa ctttccccgg
      721 atagagatac atcaccctga tcctgaaatc tccgacgacc gagttgatct ctttcaaaag
      781 catgctgagc gttgaaccgt tcacaaaacc gaattttgtc gtatcctgcg atatcagcac
      841 aacttcctta accccgtttg caacaaggtt ctccacttcg ctcactatat cgggaacacc
      901 tctttcacga tactttcccc gaataaacgg aatagaacag tacgaacaga aattctcaca
      961 gccgtctgca attttcacat agcaagtgta aggcgaatat gtgagaaccc tctccattcc
     1021 ggaattgtaa ataaattcag acggttttat tttcaaagaa gatccttttt caacaattga
     1081 tgaaatatcc gctatattat caatgccgat cacaagatca gcttcgttaa gcacattttt
     1141 cagatccgga taaagctgag gcagacaccc tgcaacaccg atgcgggcac ctttttttaa
     1201 agatgcatga agttcaaaga aacggctgac cgactcatct cttgcatcag caataaaact
     1261 gcatgtgtta agaattataa gatcagcttc ccccggatcc tcagttatca cccagccact
     1321 gttcataagc gccgcgatca tcacttctga atcaacacgg tttttcggac agccgagcga
     1381 ttcaaaaaaa agttttttca tgctgttcat cgatcagttg ccgatcataa aacgggaaat
     1441 aattactgtt ctatctgttt cctgcgattg aggaagaaaa gaaatttttg tccctttctt
     1501 tgaaaaaagg agcggaccga aatacttatt tctttcgtct tccgcatcag caatgccatt
     1561 tgtcatctta aatgcaaccg caattctgtg cttcccgtaa agctttgaaa aatctccttt
     1621 ttttgttctt tttgttatta ttacatcaag aaaaagcttc cctttttcca cttcaaaaac
     1681 aacattttca acaactgcct ctatcagttc gtctttaaca gcggcaaact catcaactga
     1741 cttccttacc ggtctgtcca gcgtctcttt tgaataacta tcagttttaa ccggaatgcc
     1801 tatatcaaca ttgttcaaca tgctgaacag tgcttcaatg ttggttgatg ggaaaaaaag
     1861 gtttttgtta atgatctcaa ttgtcatatc ggggaacatg tctatcagtt ttttccggag
     1921 tttaaccagt tccacccctt ctgccggctt gagcataacc cctccgcttt tttcactcca
     1981 tgatgaagca agaacgggaa gccatgttcc gtcaacaccc ttgattatca gaggcgaagc
     2041 cgaaataccg gaaaaaaata ctgaaataac gatcacagca gtgaccaact gtctattttt
     2101 taacattttt aactccggga tcatctttat atatcctgaa agcataataa aaagcaaaaa
     2161 ggccgaaaat tatgaacgga aagctcagat actgtcccat gtcgagagtg ccatccataa
     2221 agacctgatg ttctttgaaa aattcgacaa aaaatctgaa tgtgaaataa ccggtaagga
     2281 atattccgac atagatcccc ggctttttca gatccttaaa cacaaaagaa acggcaagca
     2341 tgatcagaag gatcgtgagc cctcctgctg cttcataaag ttgcgacgga tgtctgaccg
     2401 gccaatactg gataagacag tccatacttg atatgtcgca cttgcctgtt gcgaggttcc
     2461 tcgctatgtg agaatcagcg ctgttacgga tgaatttcca gccccaggaa gcagttgttt
     2521 ctctacctac gatctcgctg ttgaaaaggt ttccgagtcg cacaagtgtc gccccgacgc
     2581 ctgcggcgaa aactattccg tcagaaagct caaaaaattt gatcttgtat tttcttataa
     2641 aaagaagaac tgctgttata agtcctattg tagcaccgtg acttgcaaca ccgcctttat
     2701 gaattttaag gatctcaaga gggtttgaca gatagaactg cggttcatag aagaagcagt
     2761 ggacgagccg tgctccgata actactccaa gcacgcccca gacaagaaaa tcatcagcca
     2821 tcttatcttt gtatccgtac attctgagaa ttcttctcat gagaaaaaag ccgataagta
     2881 tcgtagatgc gaaaaaaatg ccgtaatatc taagctgaag agtgcctaat acggcaatga
     2941 ctggatctgc gttccataca aaataagtgt tcatgatatg ctccggatct aatttaaagt
     3001 ggttcaacat taaataacag ttttgctttt atgtcggcgg taagttttcc agtcggtctt
     3061 tgcgcacccg gaaaaaagag ataaaagctt tttgccccta tctgaaaatc aagatgtttt
     3121 atcgatttct gcaaaatgtc cttcccttcg taagtgtaca gcagttcaag cttctctgaa
     3181 tcaccgaaag tgcttgtgcc gaaaactcct actagatatc tttcttttat tttatcatca
     3241 tcactgaaat aattttcaat atttccttca aaatcaacac cttttgaaac acatccttcg
     3301 tctgaagaag aatcgcattt tgttccgtct gctttaaaac tgctgaccgg ctgagcaaaa
     3361 agttcggctt caagaacaaa actggtattt tcaacaggaa catccggctt ttcaagaatt
     3421 gtgaaagtta tgtcattgag agtcgctgaa tatattttgt cgagaaattt tttataatcc
     3481 ttcagctctt caacttctgc gagcataatg gaaattgaag tgttttcgtg tctgaccatt
     3541 acatgtgtac agctttttct ttcatccgtt gtaagatcat tcaatttaca gtgatcgatc
     3601 tgttccttct tttccttttc atccattttg cagaannnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnngctgt gtaatctatt tttcttgaaa ctgtgacacc tgttataagc
     3781 tcaagaatgt tatcaagcga catttctgaa cttgtaactg aaactgtgac atcagctccc
     3841 ggatactggc ttttcaactg attttcaaga attgtctttg aaacatcttt tgccatcaac
     3901 cctttgagaa gccccaatcc ttcatcaata tcaatctcat attcagaagg gcttgaagga
     3961 ctgtctttat cgaaaggata gtcaacagtt atcggtatca gaagaaattc cgaacatgaa
     4021 gaaaggatca aaaaagctgt taaaaaaatc acgattttgc tgatcgcttt acttttcatt
     4081 gtttttaccc gttttaagta aataatcttc cataaattta tcaaggatct cttcaccgtc
     4141 aaggactgct tcggcatttc cggtctcaaa atctgtccgg tgatccttta ccattttata
     4201 cggatgaaga acatagcttc ttatctgact gccgaaatct attcccattt tctgcttatc
     4261 attttcagag atcgcctcca ttttcttttt catttcaaac tcatacagtt tagacctgag
     4321 aattttcatt gccgttgccc ggttcttgtg ctgacttcgt tcgttctgac actgagtcac
     4381 aatgcctgtc ggaagatgag ttatccttat tgctgaatct gtagtgttga catgctgtcc
     4441 gcctgccccg cctgacctga aagtatcaac tttcagatcc ttttcatcta tttccacttc
     4501 aattgaatca tcaatttcag gagtgacgaa aacactggca aaagaggtgt gccttctctt
     4561 atttgcgtca taaggactta ttctcacaag gcggtggaca ccaatttccg ctttgaggta
     4621 tccatgtgca taatcacctt ctgcaattaa agtcacattt ttaattcccg cggcatcatc
     4681 accagtgact ttgtcaataa tctcaagctt atatcctttt ctttcgcacc atcttgtgta
     4741 cattctgaaa agcattaccg cccagtcctg cgattccctt ccgccggccc cggcattgat
     4801 cgaaacaatc gcattcagta tgtcagtctt tccgccgagc attttctgaa attcgatcct
     4861 gtcaagtatg gaattagttt catcaagaac tttctgcgct tccggctcca tagagctgtc
     4921 ttcttcaaaa agctcccgag ctatcttcaa ttcatcaaac ttcgactcga actgatcaaa
     4981 gagaacaatg ctgttctgta ttgctctctt ttctttgtta agtgccgcca tttttaagga
     5041 atcattccat atttcaggag agttaagaag attatctagc tctttaagcc ggttcctttt
     5101 tgaatcaatt ttcagcgact ctgacaatct tccggctctg ccggctatat catttgtgat
     5161 ctgctcaagt tcccttaaat tcagagccat tatttactcc cagttcaagt tttccatgtt
     5221 tatttttgct ccgtattcct ttatcatcat atctgtgtat ctatcccatt tctctgtgcc
     5281 ggactgtccg acaagctctt ctattatcag cggatatgca agattaaagc tgagatgtct
     5341 tttataggca aatccggcta tttcgattat cgtgtaatag ttatcactgt caagataagg
     5401 accgagtatt tctccaggaa ggccggaaag aacatctttt ttccagagtt catccataac
     5461 ctcttcgctg aaaagtcccc agtcaccgtt atttcttgac agagattcat cgttggaaaa
     5521 tctggccgaa agcattctta tgctggaacc ctttctgagc tgttcaacaa tatcatccgc
     5581 catttttttt gaagagaccc ttacagcata aagacggtaa agcttatccc tgatgaactt
     5641 atctttgttt tcgatgtagt atgccctcac agcttcgtca tctatcgcat agatctcttt
     5701 ttttgccctt ttcttcatat actcaatgat cgtgttcttt ctgaaattat ctatctttat
     5761 cttcacttcc ggaatacttt cgacaccctc tttcaagccg gtttcataaa gaagtttttc
     5821 tgaaagttca ttgaaaagat aatttcttct caattcatca gaatctgtaa gaccgttctg
     5881 cctgagccac agaacatact tggctttaaa atcggaaact gaaaccgcct cacccttgaa
     5941 gacatatatt atcccttcat ctttaaagct tccgcatgaa acagcgagta aaaccgcaaa
     6001 aattaaaaaa agcccctttt tcatcaaacc tccatgacag cggctgacat tttatactaa
     6061 tcatataaat attacaagaa tcggtggaaa c
//
LOCUS       DAOOYB010000175         6088 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_14915_length_6088_cov_8.885298, whole genome shotgun sequence.
ACCESSION   DAOOYB010000175 DAOOYB010000000
VERSION     DAOOYB010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6088
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14915_length_6088_cov_8.885298"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="PL056_09895"
     CDS             <1..191
                     /locus_tag="PL056_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47366.1"
                     /translation="VCFPNPASSKMGYIFGGVEGKYITYQPTGAIILKDMECYCKEYP
                     DQCLYDEYMPETPVLKKK"
     gene            435..1508
                     /gene="buk"
                     /locus_tag="PL056_09900"
     CDS             435..1508
                     /gene="buk"
                     /locus_tag="PL056_09900"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF002834.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HPM47367.1"
                     /translation="MSKLEVAVVVNPGSTSTKFALFNRDGSVYDETVRHPQEEIAKFN
                     LVSEQFDFRYGMIKQAVDKAVKEKGLKVVGSVGRGGPVKPLEGGTYRVNKSLIDDLTS
                     CKYANHASNLGGLIAHKIAEDYGLSESYIVDPVTTDNMWDKARLSGLPGIERACRVHA
                     LNIKAVARKIAEKNGDDFYKANFVVAHLGGGISVAALEGGKIVDVNDGLLGMGPFSLE
                     RAGALPLRGVMKLCYSKPEAEVKKIFSSESGFKAYIGTNDFRTIEEKVVAGDPETLKV
                     YEAFKYQIAKEIGSYCAALKGKVDGIIITGGCAYFKAFVKELEEYVGWMAPVFSYPGE
                     NELDALADGAFRVIDGKEVPKEY"
     gene            1545..3134
                     /locus_tag="PL056_09905"
     CDS             1545..3134
                     /locus_tag="PL056_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013816399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPM47368.1"
                     /translation="MLLMGDEAIAQGAMDAGASGFYAYPGTPSTEIMEFVQHSKFAAA
                     RNIHRTWSANEKTAVEGALGMSYAGKRTMSMMKHVGLNVAMDPFVNSGITGANGGCIV
                     TVADDPSMHSSQDEQDSRILGKFALMPILEPSNQQEAYDMPFYAYEISEKYKVPVMMR
                     VTTRLAHSRAGVEVREPMSQNEMKLPEDKRQFVLLPAIARKRYKMLLENQKNFLAESE
                     NSGWNRYIDGGDKTRGIIACGLAYNYLMENYPDGCPYPVIKLSQYPAPKNMIKKLVEE
                     CGEILVLEDGMPMVEEDLRGLLDRDVKVKGRITGELPRDGELDPNIVAKALGLKNAEG
                     DAVPAIVAPRPPKLCTGCGHADMYNALNAAVKAKYGDGHVFSDIGCYTLGALPPFEAI
                     NSCVDMGASITMAKGAADAGLFPAVAVIGDSTFCHSGMTSLLDAIMDNSNIIVFIADN
                     STTGMTGGQKSHAEGRVIQIVTGLGVDPAHIRVINPVRKNHEEFVKIINEELEYKGVS
                     VIIPQRECMQTLSKKTKEAKK"
     gene            3146..3727
                     /locus_tag="PL056_09910"
     CDS             3146..3727
                     /locus_tag="PL056_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HPM47369.1"
                     /translation="MKVDIILAGVGGQGILSIATVLGYAAVESGLFVKQAEVHGMSQR
                     GGDVQSHFRISDKPIFSDLVPKGKADLIVSVEPMESLRYLPYLSKDGWLVTNTNPVVN
                     IGNYPEMESVMAQIKKLPHHIAIDGDKIARDVNNLKGMNMVLAGAASVFIDLDFELMK
                     KGIEVIFGKKGEELVKSNIAALTAGREFSEKNR"
     gene            3808..4113
                     /locus_tag="PL056_09915"
     CDS             3808..4113
                     /locus_tag="PL056_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016778297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HPM47370.1"
                     /translation="MNILLKEKMSELKVICRKFDVKELFVFGSITNGHFTPESDIDFL
                     VSFEDMPPEIYADKYFDLQYALEDLFKRKIDLITVNSLSNPYLMKSINENKELIYAA"
     gene            4103..4432
                     /locus_tag="PL056_09920"
     CDS             4103..4432
                     /locus_tag="PL056_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010801557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HPM47371.1"
                     /translation="MQLDVRKNLHDIITSIDSIEEFTSEISSFSEFKKNKLVKRAVER
                     ELGIIGEAVNRIKKIAPDIEFQDARKIVDLRNLVIHGYDKVDDAIIWGVIKKHLPTLK
                     DQVVGMI"
     gene            4542..>6088
                     /locus_tag="PL056_09925"
     CDS             4542..>6088
                     /locus_tag="PL056_09925"
                     /inference="COORDINATES: protein motif:HMM:NF036774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgO family outer membrane protein"
                     /protein_id="HPM47372.1"
                     /translation="MRNVVITLLVCLIFFQLSGEQKGGFTKFILQKMDYDKSYFDAIL
                     KMKKEGRKHPRLSTEEFTSETARASFNLPVLNKYVSQKESYLSGSPLTLPPLKEGYRV
                     YINLIALLNMFQAFEKKNYNDALKYGEYIVVQNKESVGLTTEENTADYYVQLFREYYF
                     LMAASHYHLKNDIKAVEWFAKIEADADLQKLKAQINEEKKQTADPKILRLEELRKKPL
                     AVMEMLNADKNPENDWMTKGMTEVLTNELVQNTDILVVERSQLEKVYKEFELALSGVT
                     TESDAIKIGEVLSAESLLVSSFQNSGQGLLFSFRIVDSQNGLALESVSGEIIKDDDMF
                     RNIRGLVLDLFVKAGWMFPETANEIGESRVPKMTNIRELAQARLAMAASPKEAKAMYA
                     KAAKEDPALANIFEDLKAEFKDVSATVAVTPFVNVAGNSEDFWMIGALVEMMTNDLPK
                     LDFTVVERTQFDGLLNEKMGQVISSEDAVKAGQSAGADLLMMGSVFHLDKHIKIQARF
                     VEIKTGIV"
BASE COUNT         1923 a         1108 c         1481 g         1576 t
ORIGIN      
        1 aggtctgttt tcctaaccct gcaagcagta aaatgggtta catattcggc ggagttgaag
       61 ggaagtatat cacatatcag ccgacaggag cgataatact caaagacatg gaatgttact
      121 gcaaagaata tcctgaccag tgcctttatg atgaatatat gccggaaaca ccagtattaa
      181 agaagaaatg atctaaaaca cctcaacccg ataatccaaa agatgtgaag acagggaaaa
      241 ggttgaagga ttaatttctt ttcgaatcaa aacagctttc actatcagtt agaaatctat
      301 tgaaaacttt tttggggaac ttcaaaagat atttgagttg atttttttgt tttttaaaaa
      361 gttgcaaagg tgtttttgat atgctacggt ttttcagatt ttggtgtttt gatcaatttt
      421 ttgaggagag aataatgagc aagctcgaag tggcagtcgt tgtaaatccg ggttcaactt
      481 caacaaagtt cgcacttttc aacagagacg gatctgttta tgatgaaact gtaagacatc
      541 cgcaggaaga gatcgcaaag ttcaatctgg tgtctgagca gttcgatttc cgctatggaa
      601 tgatcaagca ggcggttgac aaggcggtga aggaaaaggg gctcaaagtt gttggctcag
      661 taggtagagg cggtccggta aaaccgcttg aaggcggcac ataccgtgta aataagtcat
      721 tgatagacga cctcacaagc tgtaaatatg caaaccacgc ttccaattta ggcgggctga
      781 ttgctcacaa gatcgctgaa gattacggtt tgagcgaaag ctatatcgtt gaccctgtca
      841 caactgacaa tatgtgggat aaagcgagat tgagcggact tccggggatt gaaagggcat
      901 gcagagttca tgcgctcaac atcaaagcgg ttgcaaggaa gatagctgaa aagaacggag
      961 acgattttta caaggcgaat ttcgttgtcg ctcaccttgg cggaggaatt tcagttgccg
     1021 cactggaagg gggaaagatc gttgatgtca atgacggact gcttggaatg ggcccgttca
     1081 gccttgaaag ggcaggtgct ctgccgttac gcggagttat gaagctctgt tacagcaaac
     1141 ccgaagcaga agtgaaaaag atattctcaa gcgaaagcgg tttcaaggca tatatcggca
     1201 caaacgattt cagaacgatc gaagaaaaag ttgttgcagg tgatcctgag acactgaaag
     1261 tttatgaagc gttcaaatac cagatcgcta aagagatcgg ttcatactgc gccgcactca
     1321 aaggaaaagt tgacgggata atcatcacag gcggatgtgc atatttcaag gcattcgtaa
     1381 aggaacttga agagtatgtc ggctggatgg cgcctgtttt ctcatatccg ggcgaaaatg
     1441 aacttgatgc actcgcagat ggtgcatttc gagtaattga cggtaaagaa gttccgaaag
     1501 aatactgata ttaatacttg gaggtaattg tgacaaagaa aaagatgctt ctcatgggtg
     1561 atgaggcgat cgctcagggt gcgatggatg ccggtgcgtc tggcttttat gcctatccgg
     1621 gaactccgtc aacagaaata atggaattcg ttcagcattc aaagttcgcg gcagccagga
     1681 atattcacag aacatggtct gcaaatgaaa agacagccgt tgaaggtgct ctcggcatga
     1741 gctatgcggg caaaaggaca atgtcgatga tgaaacatgt cgggctcaat gttgcgatgg
     1801 acccgtttgt caattccggc atcaccggtg cgaacggcgg ttgtatcgtc actgttgcag
     1861 atgatccgag catgcactct tcgcaggatg aacaggattc aaggatactc ggcaagtttg
     1921 ctttgatgcc tatacttgaa ccttcaaatc agcaggaagc ttatgacatg cctttctatg
     1981 cctatgagat ctctgaaaaa tataaagttc ctgtcatgat gagagtcacg acacggcttg
     2041 cccattcaag agcaggtgtc gaagtgagag aacctatgtc tcagaatgaa atgaaacttc
     2101 ctgaagacaa aaggcagttt gtccttcttc ccgccattgc aaggaaaaga tataaaatgc
     2161 tccttgaaaa tcagaagaac tttcttgcag aatctgaaaa ttcaggatgg aacaggtaca
     2221 ttgacggtgg tgacaaaaca cgcggaataa tcgcctgcgg tctggcttat aattatctga
     2281 tggaaaatta tcctgacggt tgtccttatc ctgttataaa actcagccag tacccggctc
     2341 cgaagaacat gataaagaaa cttgttgaag aatgcggtga aattcttgtt cttgaagatg
     2401 gtatgccgat ggttgaagaa gaccttcgcg gacttcttga ccgtgatgtg aaagtcaaag
     2461 ggagaataac aggtgaactt ccaagagacg gagaacttga tccgaacatc gtggcaaaag
     2521 cgctcggact taaaaatgct gaaggtgatg ccgttccagc tattgttgct cccagacctc
     2581 cgaagctttg cacaggctgt ggacatgctg atatgtacaa tgctctgaat gcggctgtaa
     2641 aagcaaaata tggtgacgga catgttttca gcgacatcgg atgttatacg ctcggtgccc
     2701 ttccgccttt tgaagcgata aacagttgtg ttgacatggg tgcatcgatc acaatggcaa
     2761 aaggtgccgc cgatgcaggc cttttcccgg ctgttgcagt aataggcgac tctactttct
     2821 gccacagcgg aatgacatct ctccttgatg ccattatgga taacagcaac ataatagttt
     2881 tcatcgctga caactcgaca accggtatga caggcgggca gaaatcgcac gctgagggca
     2941 gggttatcca gatagttaca gggctcggtg tcgatccggc tcatatcagg gttataaatc
     3001 cggtaaggaa aaatcacgag gaatttgtta aaataatcaa cgaagagctt gaatacaaag
     3061 gtgtgtcggt gatcattccg caaagagagt gcatgcagac actgagtaaa aaaaccaagg
     3121 aagcaaagaa ataggaggtg cccggatgaa agttgatatt attcttgcag gtgtcggcgg
     3181 acaggggatc ctctcgatag cgactgtcct tggatatgcc gcagttgaaa gcggactttt
     3241 tgtaaagcag gcggaagttc acggaatgag ccagagaggc ggtgatgtcc agagccattt
     3301 cagaatttct gacaaaccga tattcagcga cctcgtgccg aaagggaaag cagaccttat
     3361 cgtaagtgta gaaccgatgg aatcactcag atatcttcct tacttaagca aagacggctg
     3421 gcttgtcaca aacacaaatc ccgttgtcaa cattggaaat tatcctgaaa tggaatctgt
     3481 catggcgcaa ataaagaaac ttcctcatca tatcgccatc gacggagata agatcgcaag
     3541 agatgtgaac aacctgaaag ggatgaacat ggttcttgca ggtgctgcaa gcgttttcat
     3601 cgaccttgat tttgaactca tgaagaaagg gatcgaagtc atcttcggga aaaaaggtga
     3661 agaactcgta aaatcaaata tcgccgctct taccgccggc agagaatttt ctgaaaagaa
     3721 taggtaagat cttagattta ttttccactt gatttgacaa ttaaacaacg catcaatata
     3781 attaatatgt tttttaaagg aggcgttatg aacatattgc tcaaagaaaa aatgagtgaa
     3841 cttaaggtta tatgcagaaa attcgatgta aaggagcttt ttgttttcgg ttcaattacc
     3901 aacggtcatt tcacaccgga gagcgatatt gactttctcg tttcttttga ggatatgccg
     3961 cctgagattt atgctgataa atattttgat cttcaatatg cactggaaga cctttttaaa
     4021 aggaagatcg atctcataac agtcaattca ctttcaaacc cgtatctaat gaaatctatc
     4081 aatgaaaata aagagttgat ttatgcagct tgatgtaagg aagaatctcc acgatataat
     4141 cacatccatt gactcaattg aagagtttac ttcagaaatt tcgagttttt cagaatttaa
     4201 gaagaacaag cttgtgaaaa gagcagtgga aagagaacta ggaataatag gcgaagcggt
     4261 aaacagaata aaaaagattg ccccggatat tgaatttcaa gatgcaagaa agattgttga
     4321 tttacgaaat ctggttattc acggatatga caaagttgac gatgccataa tctggggagt
     4381 aataaaaaaa catttaccga ctttgaaaga tcaagtcgtc ggaatgattt gaaatctaag
     4441 aatttatctt atctcctgga ttttctttac aacaaaataa ccgtccttga ttaatattat
     4501 cagagtgata caatgtgctg aatttattaa aggaggttcc gatgcggaat gttgtaatca
     4561 cattgctggt ctgccttata tttttccagt tatcaggaga acagaagggc ggattcacaa
     4621 agttcattct gcagaaaatg gattatgata agagctattt tgacgctatt ctgaaaatga
     4681 aaaaagaggg gagaaaacat ccccgtctca gcactgagga gtttacatca gaaacggcaa
     4741 gagcttcttt caacctgcct gttttaaata aatatgtgtc acagaaggaa tcttatcttt
     4801 cagggagtcc tctcacactt cctccgttaa aagagggata ccgcgtctat atcaatctga
     4861 tcgctcttct taacatgttc caggcatttg aaaagaagaa ttataatgac gcgctcaaat
     4921 acggtgaata tatcgttgtt cagaataagg aaagtgtcgg gctgacaact gaagagaata
     4981 ctgctgacta ctatgttcag cttttcagag aatattattt tctgatggcg gcaagtcact
     5041 accatttgaa aaacgacata aaagcggtcg agtggtttgc aaagatcgaa gccgatgccg
     5101 atctgcagaa gttgaaagct cagatcaatg aagagaaaaa gcagactgct gatcctaaaa
     5161 tcctgcggct tgaggaattg agaaaaaaac cgctcgctgt aatggaaatg ctcaatgcag
     5221 ataaaaatcc cgaaaatgac tggatgacaa agggaatgac cgaagtgctc acaaatgaac
     5281 tcgttcagaa cacagatatt cttgtggttg agaggagtca gcttgaaaag gtttataaag
     5341 aatttgaact ggcactttcg ggtgttacga cggaatctga tgcgataaag atcggtgaag
     5401 tgctcagtgc tgaatcactt ctggtaagct ctttccagaa tagcggacag gggcttcttt
     5461 ttagtttcag aatagtggat tcacagaatg gactggctct ggaatcagtg agcggagaga
     5521 tcattaaaga tgatgatatg ttcaggaaca tcagaggact tgtgctcgat ctgtttgtga
     5581 aggcagggtg gatgtttcct gaaactgcaa atgagatagg agaatcaaga gtgccgaaaa
     5641 tgaccaatat cagggaactt gcacaggcgc ggcttgcaat ggcggcaagt ccgaaagagg
     5701 caaaggcaat gtatgcaaaa gcggcgaaag aagatccggc attagccaat atttttgaag
     5761 atcttaaagc ggaatttaaa gatgtttcag caactgtagc agtaacaccg tttgtaaatg
     5821 ttgcagggaa cagtgaagat ttctggatga taggcgccct tgtcgaaatg atgacaaacg
     5881 atctgcctaa acttgatttt actgttgtcg agcggactca gtttgacgga ttgttaaatg
     5941 aaaaaatggg acaggttata tcaagcgaag atgctgtaaa agcaggtcaa agtgccggag
     6001 cggacctttt aatgatggga agtgtttttc atctggataa gcatataaaa attcaggcaa
     6061 gattcgttga gataaaaaca gggatcgt
//
LOCUS       DAOOYB010000176         6015 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_15165_length_6015_cov_10.000336, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000176 DAOOYB010000000
VERSION     DAOOYB010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6015
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15165_length_6015_cov_10.000336"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="PL056_09930"
     CDS             <1..185
                     /locus_tag="PL056_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016199629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1016 domain-containing protein"
                     /protein_id="HPM47373.1"
                     /translation="IGILLSAGKNDAVVRYTLPENNRTILASQYDLYLPSEESLKNEM
                     TKTLENRKRRNAPPQS"
     gene            295..2292
                     /locus_tag="PL056_09935"
     CDS             295..2292
                     /locus_tag="PL056_09935"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:NF015022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HPM47374.1"
                     /translation="MSEKPKKFIFTGGGSGGHVSPALAVAAELKKKYPSSNILYVGVK
                     NKAEGLMVPKAGIPLKFVNSAPFPGKSPVAVVKFVIAMVLGIIKAKLILLTFRPDVVF
                     ATGGYVSAPIVFTAWMLRKFTFGLFKTKILIHESNVHPGKMNKWAASAADEVAVSFNE
                     TIKCLPKGKGFFSGYPVRSTIAAGDKDSARHELGIPENAFVVFAFGGSQGARAINRAL
                     ADAAAELLKEPDIYIVHGTGKPFGENYEYHGFNDVQSILKEKHPQLLNEKRYMIKDFI
                     DNMGLYYAASDLLVIRAGAGSIMEVCRQGKPSIIIPISGIYSDHQTGNARYIERAGAA
                     KVIYEKTDPVNGINTPFVDSAELAETVNDLKPDQFKLYAMSKTATTIFPGNPAEIIVD
                     HILYFCGIGEKPGAVEGVTYQQDRILGLGSVALEQFMKNIKNGTEKKLDSDEMRLLKN
                     KIDSYLSSGSVILKARGLRISGLANYCEMLPLVVRSATGDHEKPFVRRDAFDALSKLT
                     SCNRDMRHEIFSAVMTGLDDKYYEARFAAARCIPVFLKQYGLQSEETEKLLEKLYANL
                     DHRCFEVRVESILAVSWIENDGARVLKQFSRCYFDKVWKVREAIYKGMGKLVERHVID
                     PDTALKEMDSILITSNGYLTEYELKKQYNKSRSVISKEVGK"
     gene            2289..>2629
                     /locus_tag="PL056_09940"
     CDS             2289..>2629
                     /locus_tag="PL056_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47375.1"
                     /translation="MIYNIFQSFFETGGLAVFKYITSRTLISFVTAFFLTLLIGRPLI
                     ILLYKKGFRSFERSYGDINAQSKKGTPMMGGIIMFITGIGSAVLWCDLTNPFILMLLA
                     SAVWFGLFGGI"
     assembly_gap    2630..2729
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2730..3197
                     /locus_tag="PL056_09945"
     CDS             <2730..3197
                     /locus_tag="PL056_09945"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HPM47376.1"
                     /translation="IFIFIVIGVFSYIIGNVIHAQHLLYYYIPGSGEIAVFCGAIIGA
                     GLGFLWFNTHPAEVFMGDMGSLFLGGTMGTAAVLIRQEVVYIIAGAIFVIEITSTFVQ
                     ENIGFKMYRRILFRAPLHHHFHHQGMAEAKIVSRFWIISAIMAAIAIITIKFR"
     gene            3202..3618
                     /locus_tag="PL056_09950"
     CDS             3202..3618
                     /locus_tag="PL056_09950"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HPM47377.1"
                     /translation="MPKKQKMYFNADDCRFCNGMCCRYVLIDIQKEPRSKKSFDELVW
                     WLFNPNIKILKNGRDWSILVMGKCENLDGFNKCAIYEHRPDPCRDYPYPVEEGCHFND
                     IRNLDGIIFNNAKELLEYLAKTRKKEWAKIWLENIS"
     gene            3716..4930
                     /locus_tag="PL056_09955"
                     /pseudo
     CDS             3716..4930
                     /locus_tag="PL056_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002708229.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
     gene            4979..5209
                     /locus_tag="PL056_09960"
     CDS             4979..5209
                     /locus_tag="PL056_09960"
                     /inference="COORDINATES: protein motif:HMM:NF024804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPM47378.1"
                     /translation="MSTNASINALINAPLTRLQKEILEIIRNNEFVTYEEIADVTKRD
                     RSTVMRNVQKLKGLNLIKRSGSRKTGRWIILF"
     gene            5220..>6015
                     /locus_tag="PL056_09965"
     CDS             5220..>6015
                     /locus_tag="PL056_09965"
                     /inference="COORDINATES: protein motif:HMM:NF012395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005544 - calcium-dependent phospholipid
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C2 domain-containing protein"
                     /protein_id="HPM47379.1"
                     /translation="MKKILILLTIFIVIFISSCGGEEETSDVINCVDKYDCPETMQCI
                     DGVCSDKCVDNSDCPMNLYCYDGECKEKQVVDKDENIPDEMVVADENEVVPDESEEPD
                     NEVNDEDSTDKPIPCTEHENCPEDKYCFKNYCINPFLNKWKIGLIDLCVNDENGEGDN
                     WDPAISFKPEPEPYVVAYFNDKQIFQTTPPDDSHCATFTDTFETFLISGDVLKFDVYD
                     EDDGLDVFTGDDHIDTITVTPILVEYLRNGSYSATGMNNMKTFNFVI"
BASE COUNT         1821 a         1073 c         1380 g         1641 t
ORIGIN      
        1 cgataggcat tcttctttct gctggaaaaa acgatgcagt agtgagatac actcttcccg
       61 aaaacaacag aacaattctt gcaagtcagt atgatcttta tctcccgagc gaagaatcac
      121 tgaaaaacga aatgactaaa acattggaaa accgcaaaag aaggaatgct ccacctcaat
      181 cataaaaccg accgcatttt ttctaattat tccttattct cttgttaaaa tattgtatta
      241 tttactatat ctgataaata tttgtgaatt gtttgattat tgccgggtga gaaaatgagt
      301 gaaaaaccga aaaagttcat atttacgggt ggtggaagcg gtggtcatgt gagtcctgct
      361 cttgctgttg cggctgaact gaaaaaaaaa tatccttctt caaacattct ttatgtcggt
      421 gtgaagaaca aggcggaagg gctgatggtg ccgaaagcgg ggataccgct gaagttcgtc
      481 aacagcgccc ctttcccggg aaaatctccg gtggcggtcg ttaaattcgt aattgcgatg
      541 gtgctcggga taataaaggc aaagctcatt ctcttaacat tcaggcccga tgtggttttt
      601 gcaacaggcg gatatgtcag tgccccgata gttttcactg catggatgct cagaaagttc
      661 actttcggac tttttaagac taaaattctc attcatgaat caaatgtcca tccgggaaaa
      721 atgaacaagt gggcggcttc tgcggccgat gaagtggcgg tctctttcaa tgaaacgatc
      781 aaatgcctgc cgaaaggaaa aggtttcttt tcgggctacc ctgtcagaag cacgatcgct
      841 gcaggagaca aggattctgc aagacatgaa ctcggcatac ctgaaaatgc ttttgttgtt
      901 ttcgcgttcg gcggatctca gggtgcaagg gcgattaacc gtgctcttgc cgatgcggcg
      961 gcagaacttt tgaaagagcc agacatttac atagttcacg gaaccggaaa accgttcggt
     1021 gaaaattatg aatatcacgg attcaatgat gttcagtcca ttctgaaaga gaaacacccg
     1081 caactgctta atgaaaaaag atatatgatc aaggacttta tagacaacat gggtctttat
     1141 tatgcggcaa gcgatcttct tgtaataaga gcgggagccg gctccatcat ggaagtgtgc
     1201 cgtcagggta aaccttcgat tataattcct atcagcggaa tatacagcga tcaccagaca
     1261 gggaatgcga gatatatcga aagggccggt gcggcaaagg ttatttatga aaaaacagat
     1321 ccggtgaacg gaataaacac cccttttgta gacagtgctg aacttgcgga aactgttaat
     1381 gacctgaagc cggatcagtt taagctttat gcgatgtcaa agactgcaac aacgatattt
     1441 ccgggaaatc ctgctgaaat aatagttgat catatcctct atttctgcgg gatcggtgaa
     1501 aaacccggag ctgtcgaagg ggtgacatat cagcaggata gaattctcgg gcttggttca
     1561 gttgcacttg agcagttcat gaaaaatatt aaaaacggta ccgaaaaaaa gctcgattct
     1621 gatgaaatgc ggcttctgaa aaacaaaatt gattcatatc tttcaagcgg aagcgtgatc
     1681 ctcaaagcaa gaggacttag aatttcaggc cttgcaaact actgcgaaat gcttcctctt
     1741 gtggtaaggt cggcaacagg cgatcacgaa aaaccgtttg tgagaagaga tgcgtttgac
     1801 gcactttcaa agctcacttc atgcaacaga gatatgagac acgaaatatt cagtgccgtc
     1861 atgaccggcc ttgacgataa gtattacgaa gctagatttg ctgccgcaag atgtattcct
     1921 gtgtttttaa agcagtacgg acttcaaagc gaagaaacag aaaaacttct tgaaaaactt
     1981 tatgcaaacc ttgaccacag atgttttgaa gtaagagttg aatcgatcct tgcggtttcg
     2041 tggattgaaa atgacggagc aagggtttta aaacagttta gcagatgcta tttcgacaaa
     2101 gtatggaagg tgagggaagc gatttataaa ggtatgggaa aacttgtgga aaggcatgtt
     2161 atagatccgg acacagcact gaaggaaatg gacagcatcc tcatcacaag caacggatat
     2221 ctgacagaat atgagctcaa gaagcagtac aacaagagcc gttcagtaat ttcaaaggag
     2281 gtcggaaaat gatatacaac atctttcagt cgttttttga aactggcgga cttgcagttt
     2341 ttaaatatat cacttcccgg actctgatct catttgttac cgcatttttt ctgactctgt
     2401 taataggcag acctttgata attctcttat acaaaaaggg tttccgttct tttgaacggt
     2461 cttacggcga cataaatgct cagtcaaaga aagggactcc gatgatgggc gggataatta
     2521 tgttcataac cgggatcggg agcgctgtgc tctggtgcga cctgacgaat ccgtttattc
     2581 tgatgctcct tgcgagtgcc gtgtggttcg gacttttcgg cggaattgan nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnna tcttcatttt cattgtgatc ggtgtttttt
     2761 cttatatcat cggaaatgtc atacatgctc agcatcttct ttattattac attcccgggt
     2821 ctggagaaat tgcagtattt tgcggagcta taattggagc aggtcttgga tttttatggt
     2881 tcaacactca tcctgccgaa gttttcatgg gtgacatggg aagtcttttc cttggcggaa
     2941 caatgggaac agcggcagtt ctgataaggc aggaagtggt ttatatcatt gccggagcca
     3001 tatttgtgat agagataact tcgacattcg ttcaggagaa tatcggtttt aaaatgtacc
     3061 gccggattct tttcagagca ccgctccacc accattttca tcatcaggga atggctgaag
     3121 ccaagatcgt ttcaaggttc tggatcattt cggccatcat ggcggcgata gcaataatta
     3181 caataaaatt cagatagtta tatgccaaaa aaacagaaga tgtatttcaa tgcagatgac
     3241 tgcagattct gcaacggaat gtgctgcagg tatgttctca ttgacattca aaaggagccg
     3301 agatcaaaaa aaagttttga cgaacttgtc tggtggcttt tcaacccgaa cataaaaatc
     3361 ttaaagaacg gtcgcgactg gtcaattctt gtcatgggta aatgcgaaaa ccttgatgga
     3421 ttcaacaaat gcgctattta tgaacaccgt cctgatccat gccgggatta tccatatccg
     3481 gttgaggaag gatgtcattt caacgatatc cgtaatcttg acggcataat tttcaacaac
     3541 gcaaaagaac ttcttgaata tcttgccaaa accagaaaaa aagagtgggc aaaaatttgg
     3601 ctggaaaata tttcgtaaag atttgcacct ataaactttt aaagatgcag aaaataagta
     3661 taatgcacct ataaaggttt ccggtttttt ataaaaacgg ttccggaggt tgaagatgcg
     3721 gaaagaggaa ttgcttgaaa taattagcaa cggtgagaat tcgagcgtgg aatttaagct
     3781 tgatgagatc aggcctgaac agcttgcaaa agagatagtc gcccttgcaa attttcatgg
     3841 cggtaaaatc tttataggtg ttgcagataa tggagaaata gaaggaatta aaagaaaaaa
     3901 tattgaagaa tgggttatgg atacagtttt tggaagatat gttcatccgg taataattcc
     3961 tttttatgaa gaaattgaac ttgatggaag gaaggttgct gttgtttctt ttccttcagg
     4021 gatctcaaag ccttatgttg tcagaaacaa tggtcgtgaa gatgtgtaca ttagaatagg
     4081 gagcacatcg cggcttgcaa caagagaaca gcaggcacaa ctttattctg ttggaggaat
     4141 gctccacact gaggtaatgc cggttcccgg aacttcttta agctctcttg atcatgtccg
     4201 gcttgaaaat tatatttacg atatacttgg cgagccttct tctattatat cgaaggaaga
     4261 gtggatcgat cgtttgagcg gacttggttt tattataaag agtgatgacg gcagaattct
     4321 ttcaacggtt gcaggactgc ttctttttgg aataaatccg aggcgttatt taaagaatgc
     4381 agggttgcgt gttatggttt tcccgggcgg agataaagag tatagagctc ttcttgatga
     4441 aattattgac agtccgcttg taggaagatg gaaagtttca tcaggcgtta aagagctgat
     4501 cgatgccggg cttattgaaa aatttatttt tgctttatcc ccttttattt ctgaagaatc
     4561 tgacaaaatt gacaacaacc tcaggcgtga aaaaaaattc atttatcctg ttgaagctat
     4621 ccgggaaaca gttgtcaatg ctttcgctca cagagactgg acccgttttg cagatgttga
     4681 agttgccggc tattcagacc gtcttgaagt gataagcccc ggggcatttc aaaattcaat
     4741 gacgatcgaa aaaatgatag cgggacagag gtgtgcaaga aaccctgtaa ttgttgaggt
     4801 gttgagagat tacggttatg ttgatgcacg gggaatgggg atcaggacaa aagtaatacc
     4861 gttaatgaaa aagttcttaa acaaagatcc ggtttttaaa gcgacagagg attatgtttc
     4921 agttacactt ccaaattcag aaacaggttt tgtaaaagag gtgcggacag aatataatgt
     4981 gtccacaaat gcatcaataa atgcactaat aaatgcgcct ttaacccggc ttcagaaaga
     5041 aattcttgaa atcatcagga acaatgaatt tgttacttat gaagagatcg cagatgttac
     5101 aaaaagggat cgcagtactg tcatgaggaa tgtgcaaaag cttaaaggtt tgaatttaat
     5161 aaagagatcc ggttcaagaa aaacaggcag atggataatt ttattttgag ggataaacga
     5221 tgaaaaagat tttaatcctg ctcacaattt ttattgtcat tttcatctct tcctgcggcg
     5281 gggaggagga gacaagtgat gtgataaact gcgttgacaa gtacgactgt ccggagacaa
     5341 tgcagtgcat tgacggggtg tgcagtgata agtgtgttga caattccgac tgcccgatga
     5401 atctctattg ctatgacgga gaatgcaagg aaaaacaggt ggttgataaa gatgaaaaca
     5461 tacctgatga aatggtcgtt gctgatgaaa acgaagttgt tcctgatgaa tctgaagagc
     5521 cggataacga agtgaatgat gaagattcaa ctgataaacc gattccctgt acagaacatg
     5581 aaaactgccc tgaagataaa tactgcttca agaattactg cataaatcct tttttaaata
     5641 agtggaagat cggacttatt gatctgtgtg tcaacgatga gaacggggaa ggggataact
     5701 gggacccggc aatttcgttc aagcccgagc cggaaccata tgtcgttgca tatttcaatg
     5761 ataagcagat cttccagaca actccgcctg atgattcaca ctgtgcgact tttactgaca
     5821 cttttgaaac atttctcatt tccggagatg tactgaaatt cgatgtctat gacgaagatg
     5881 acggacttga tgtttttacg ggagacgacc atattgacac gataacggtt actccgatcc
     5941 ttgttgaata cctgagaaac ggaagttatt cagcaaccgg catgaacaac atgaaaacct
     6001 tcaattttgt catca
//
LOCUS       DAOOYB010000177         6008 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_15197_length_6008_cov_10.011759, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000177 DAOOYB010000000
VERSION     DAOOYB010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6008
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15197_length_6008_cov_10.011759"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="PL056_09970"
     CDS             complement(<1..398)
                     /locus_tag="PL056_09970"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HPM47380.1"
                     /translation="MKDPRVRKLAELLVNYSTKVKENDKVMVAAVDPAAYPLVEEIIE
                     LVGRKGAHPFVTLNAQAVQRKLYMHATDTMLDIMRESELAFAQKMDAYIGIRALENKF
                     ETNDVPQKQMMKIMQNYRMKVSNEIMSKKWV"
     gene            553..2376
                     /locus_tag="PL056_09975"
     CDS             553..2376
                     /locus_tag="PL056_09975"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM47381.1"
                     /translation="MGIFKFFKKEDSDIKLPELLNRYKNSDLEIRIKVKFIFVLCIIM
                     MIFIVPAMTYTAWIHFSFVSSPEIMIPILTGEAAGIMFLFIALILLIKGFHKLASHLI
                     IISSILSIWLVMIVDRSATLIRLDTATYILAVLALMPAFFVRKRYIPVYAAVNVIFLW
                     AYIYFLMEFNYETADYFADYTVVIIFITIVGYNIARINRTILNKYEGEIRKLKVAEEQ
                     LSNEKEKLLEAQKLLESAAQTQKLQSLGILAGGIAHDFNNLLTGIFGYIGFARDESKE
                     KNIKELLAQASGAIERAKSLTHQLLTFSKGGVPLKKLQPFSPFIEEVVRFALSGSSVT
                     PVFKISDDLKMAEFDRNQAAQVIDNIILNAVEAMSKAGKIEISAQNVALKENELFPLT
                     EGEYVKISIADEGPGIPQDSLPHIFDPFFTTKSEGHGLGLAMSYSIIRKHEGLITVDS
                     NIGKGAVFTIYIPAAAPQPVIEPPVVEHKEHGSFAGRVLVMDDERIISEIVAHILNRA
                     GFDAVTVSNGEDAIKEFSKAKREGRPFRFLIFDLTIPNGMGGQKALDRIRETDKNVIA
                     FVSTGYSDDPVVADPAAYGFNDTISKPFEIDELKEKLGKYL"
     gene            2392..>3953
                     /gene="pgi"
                     /locus_tag="PL056_09980"
     CDS             2392..>3953
                     /gene="pgi"
                     /locus_tag="PL056_09980"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013410060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HPM47382.1"
                     /translation="MLKKIEPVKLASWKKLEKLRSVKEFNLRKMFASDPLRADRYSIE
                     FNDIFVDYSKNLIDDDVLKDLFELAAECGLKGAVNSMFEGEKINETENRAVLHTALRN
                     FSDRPVIESGTDVMPQIRKVLEKMKKFADIVSNGKWIGFKGEAVTDIVNIGIGGSDLG
                     PVMVTEALKPYWKKGMKVHFVSNVDGTHIAETLKKLKPETTLFLIASKTFTTQETMTN
                     AESAKKWFLWNGGEPADIAKHFAALSTNTKAVTEFGIDPENMFEFWDWVGGRYSLWSA
                     IGLSIACVTGYENFERLLKGAYLMDEHFRSEPFERNVPVILGLLGIWYNNFFDADSHA
                     VLPYDQYMHRFAAYLQQGDMESNGKCVDRSGNTVTYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKLIPCDFLAPAVTHNPVSDHHEKLLANFFAQPEALMNGKTEEEAEKELEKAGKT
                     AQEIAKLLPFKVFSGNRPTNSIIFKKLTPEVLGSLIAAYEHKIFVQGVIWNVFSFDQW
                     GVELGKQLAGKIL"
     assembly_gap    3954..4053
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4055..4618)
                     /locus_tag="PL056_09985"
     CDS             complement(4055..4618)
                     /locus_tag="PL056_09985"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012194488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HPM47383.1"
                     /translation="MKTILINKNTVLKQIELSDTADIFETVISQREYLGKWLPFVTFT
                     EEMKDTEDFVRSVVDTPEDVREFVFVIHYKGEFAGLIGFKDTDRLNRKTEIGYWLSEG
                     FQKKGIMTESVRFLIKFVFEKLSINRIQIRCAVGNTPSSNIPKRLGFKFEGIEREGEF
                     FPDGTFKDLEVYGMVKGEYFPGPSTAG"
     gene            complement(4630..5307)
                     /locus_tag="PL056_09990"
     CDS             complement(4630..5307)
                     /locus_tag="PL056_09990"
                     /inference="COORDINATES: protein motif:HMM:NF024098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HPM47384.1"
                     /translation="MIHWIKKSRCYEVEKLRNTTLLSLQKSQNHKLYAQTKKNYAKTR
                     PYIHLTYNERLDFIKEVAGKISSWSFVRLFAECIDKIHFDPSKAKLSVDEQALEQIIS
                     RFEYCLKSVSKTSKSKKYGLIIHDNNETVARKHTELMKRFHRNGTIWTKIENIIETPL
                     FVNSELTGMIQLSDLCCYAIRRYLENGERELFDLVFKRGDRKNEKCVGIRHFSNNRCE
                     CVICKSH"
     gene            complement(5267..5521)
                     /locus_tag="PL056_09995"
     CDS             complement(5267..5521)
                     /locus_tag="PL056_09995"
                     /inference="COORDINATES: protein motif:HMM:NF024098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HPM47385.1"
                     /translation="MYLCYIDESGTPDIPGNTSHYVLSGLAIPIDKWKKCDVEINLIK
                     KKYDLENEEIHTGWILRKYLEQSKIVNFDSLDKKKQMLRS"
     gene            5737..>6008
                     /locus_tag="PL056_10000"
     CDS             5737..>6008
                     /locus_tag="PL056_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47386.1"
                     /translation="MSIFKHIREIKRNKRLFDEKVYEMVLDEIAQDQKQPGLWAKAIA
                     DSSGNMDKAKSLYIKYRAEAIKQDIDFLKDNELKTERNETQPKTET"
BASE COUNT         1812 a         1135 c         1178 g         1783 t
ORIGIN      
        1 acccattttt tgctcattat ttcgttactt accttcatcc ggtagttctg cattattttc
       61 atcatctgtt tctgcggaac atcgtttgtc tcaaatttgt tttcaagtgc tcttatcccg
      121 atgtatgcgt ccattttctg tgcaaatgca agttccgatt cacgcatgat atcaagcatc
      181 gtgtcagttg catgcatata aagttttctc tgaaccgcct gggcattaag agttacaaaa
      241 ggatgagccc cttttctgcc gacaagttca atgatctctt caacaagcgg ataagcggcc
      301 ggatcaactg ccgcgaccat aactttgtca ttttccttaa cttttgtgga ataatttaca
      361 agaagttctg caagttttct tactctcggg tctttcattt ccatctcctt agaagtgtta
      421 aaaaactgaa gctgacatat attaaccaca ttaaaatttt aggcaaaaat attgtgtctg
      481 attttaattt tacttgcttt tttatgctga aaaaagtaat tttttacagg tcggtatttt
      541 gagaaaagca aaatgggaat tttcaaattt tttaagaaag aagatagtga cataaaactt
      601 cctgagttgc tcaaccgtta caaaaattct gatctggaga tcaggatcaa ggtgaagttc
      661 atctttgttt tatgcattat catgatgata tttattgttc cggcaatgac atataccgca
      721 tggatacatt tttcttttgt ttcaagtccg gagatcatga tccccatcct taccggggag
      781 gcggcaggca taatgtttct tttcatcgct ttgatccttc tcataaaggg tttccacaaa
      841 ttagcgtccc atcttataat catttcttcg atactttcga tatggcttgt gatgatagtt
      901 gaccgttccg ctaccttgat ccgtcttgac acagcgacat atattcttgc tgttcttgca
      961 ttaatgccgg cttttttcgt gaggaagcgg tatatccctg tttatgcggc tgtaaatgtc
     1021 atttttcttt gggcatatat ctattttctg atggagttta actatgagac agcagattat
     1081 tttgccgatt atactgtagt gataattttt ataacgatcg tcggatataa cattgcaagg
     1141 ataaaccgga caattcttaa caaatatgaa ggggagataa gaaagcttaa agttgcggaa
     1201 gaacagcttt caaatgaaaa agagaaactt cttgaagcac agaaacttct tgaatcagcg
     1261 gctcagacgc agaaactgca atctcttgga atacttgcag gcggcatagc acacgatttc
     1321 aacaatcttc tgacaggtat tttcggatat atcggttttg caagagatga atcaaaggag
     1381 aagaatatta aagaacttct tgctcaggca tcaggtgcaa ttgagagggc aaaatcgcta
     1441 acacaccagc ttctgacatt ttccaaaggg ggcgttcctt taaaaaaact gcaacctttt
     1501 tctcctttta ttgaagaagt tgtccgtttc gcactcagcg gttcatcggt cacaccggtg
     1561 tttaagatat cagatgatct taaaatggcg gaatttgaca gaaatcaggc tgctcaagtc
     1621 attgataaca ttatcttaaa tgccgtagaa gcaatgtcaa aggcagggaa gatagaaata
     1681 tctgcacaaa atgtcgcact taaagaaaat gagctgttcc ctctgactga aggggaatat
     1741 gttaaaattt caatagctga cgaaggaccg ggaattccgc aggattctct accccacatt
     1801 ttcgatcctt ttttcacaac aaaaagtgag ggtcacggac tcggtcttgc aatgagctat
     1861 tccatcatca gaaaacatga ggggttgata acggttgatt caaatattgg taaaggtgct
     1921 gttttcacga tatacattcc tgccgcggca cctcaacctg ttattgagcc gccggtcgtt
     1981 gagcataaag aacacggctc ttttgcagga agagtgcttg tgatggatga tgaaaggata
     2041 atttctgaaa tagtggctca tatccttaac cgtgccggtt ttgatgctgt aactgtgagt
     2101 aacggagaag atgcgatcaa agagttcagc aaggcaaaaa gagaggggag acctttcaga
     2161 tttctgatat ttgatctcac catccccaac ggaatggggg gacagaaagc acttgacaga
     2221 ataagggaaa cggataagaa tgtcattgct tttgtaagca ccggctacag cgatgatccc
     2281 gttgttgcag atcctgcggc ttacggtttt aatgacacta tctcaaaacc gtttgaaatt
     2341 gatgaactga aggaaaaact gggtaaatat ttgtgatata agggggcata aatgcttaaa
     2401 aagatcgagc cggttaaact tgcgtcgtgg aaaaagcttg aaaagttaag atcagtcaag
     2461 gagtttaact taagaaaaat gtttgcctca gatccattga gagccgacag gtattccatt
     2521 gaatttaatg acatttttgt cgattattca aagaacctga tcgatgatga tgttttgaaa
     2581 gacctttttg aacttgccgc ggagtgcggt ttaaaaggag cggtaaattc aatgtttgaa
     2641 ggggaaaaga tcaatgaaac tgaaaaccgc gccgtgcttc acactgctct tagaaatttt
     2701 tcagatagac cagtgatcga aagcggaacg gatgtgatgc cgcaaatcag aaaagtgctt
     2761 gaaaagatga agaaatttgc cgatattgtc agcaatggta aatggatagg gttcaaaggg
     2821 gaagcagtga cggacattgt aaatatcggt atcggaggaa gcgatctcgg tcctgtgatg
     2881 gtgactgaag cgctgaagcc atactggaaa aaggggatga aggtccattt cgtttctaat
     2941 gttgacggaa ctcatatagc agaaactctt aaaaagctta agccggagac gacacttttc
     3001 ctcattgcat ctaaaacttt cacaacccag gaaaccatga caaatgctga gagtgcaaaa
     3061 aagtggttcc tgtggaatgg cggggagccg gctgatattg ctaaacattt tgctgctctt
     3121 tcaacaaaca caaaagctgt gaccgagttt ggaattgatc ctgagaatat gtttgaattc
     3181 tgggactggg ttggcggaag atattctctg tggagtgcaa taggattaag cattgcctgt
     3241 gttaccggat atgaaaattt tgaaagactg ctcaaaggtg cttatttaat ggatgagcat
     3301 ttcagatctg agccgtttga aagaaatgtt cctgtgatat tagggctttt gggaatctgg
     3361 tataacaact tttttgatgc ggattctcac gcagttctcc cttatgacca gtatatgcac
     3421 cggtttgccg catatcttca gcagggagat atggaatcaa atggaaaatg tgtggataga
     3481 tcgggaaata cagttactta ccagacaggt ccgatcatct ggggagagcc cgggacaaac
     3541 ggtcagcacg cattttatca gctcattcat caggggacaa agctcattcc gtgcgatttt
     3601 cttgctccgg cagtaactca taatcctgtt agcgatcacc acgaaaagct tcttgccaat
     3661 ttttttgccc agcccgaagc gctcatgaac ggaaaaactg aagaagaagc agaaaaagag
     3721 cttgaaaaag ctggaaaaac ggctcaagag atagcaaaat tacttccttt caaagtgttt
     3781 tcaggaaaca ggcctactaa ttcgataata tttaaaaaac tcacacccga agttctcgga
     3841 tctctcattg cggcgtatga acacaagatc tttgttcagg gagtcatctg gaatgtgttc
     3901 agttttgacc agtggggagt tgaactcggc aaacagcttg caggcaaaat tctnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncttagcc cgcggttgaa ggcccgggaa
     4081 aatattcccc cttcaccatt ccgtaaactt caaggtcctt aaaagtccca tcgggaaaaa
     4141 actctccttc cctttcgatc ccttcgaact taaaccccag tcttttcgga atgttactgc
     4201 tcggagtgtt gccgacagcg catctgatct ggatacggtt aatgctcagt ttttcaaaaa
     4261 cgaattttat aaggaacctc acagattccg tcattatccc ctttttctga aaaccctcac
     4321 tcagccagta cccgatctct gtcttcctgt tcaatctgtc agtatcctta aacccgataa
     4381 gccccgcaaa ttcacctttg taatgaataa cgaaaacaaa ctctctgaca tcttccggag
     4441 tatccacgac tgaccggaca aaatcctcag tgtccttcat ctcctccgta aaagtgacaa
     4501 acggaagcca tttcccgaga tattcccgct gagaaattac cgtttcaaaa atatccgccg
     4561 tgtcagacaa ttctatctgc tttaaaaccg tgtttttgtt gatgagtatt gttttcatgg
     4621 agcgtgatat caatgagatt tacaaattac acattcacaa cgattgttgg aaaaatgtct
     4681 tattccgaca catttttcgt tttttctatc acctcgcttg aaaacgagat caaaaagctc
     4741 tctttcacca ttttccaaat atcttcttat cgcataacag cataaatctg atagctgtat
     4801 cattccagtc aattctgaat ttacaaataa aggggtttca attatatttt caatttttgt
     4861 ccaaatagtt ccattccgat gaaatctttt catcaattcg gtatgctttc ttgccacagt
     4921 ttcattattg tcatgaataa taagcccgta ttttttagat ttacttgttt tagatacact
     4981 tttcaaacaa tattcaaatc ttgatataat ttgctccaaa gcctgctcat ctacagaaag
     5041 cttagcctta gaaggatcaa aatgaatttt atcaatgcac tctgcaaaca atcttacaaa
     5101 agaccaactt gaaattttac ctgcaacttc ttttataaaa tcaagtcttt cattatatgt
     5161 aagatgtatg taaggtctag ttttagcgta attcttcttt gtttgggcat acagcttgtg
     5221 gttttgtgat ttttgaagcg ataaaagtgt tgtgtttctt aatttctcaa cttcgtaaca
     5281 tctgcttttt tttatccaat gaatcaaagt tcacaatttt ggattgttct aaatatttac
     5341 gcagaatcca accagtatga atttcttcat tttcaagatc atactttttc ttaataagat
     5401 taatttccac atcacatttc ttccacttat caatcggaat tgctaaacca gaaaggacat
     5461 aatgcgaagt atttccagga atatcgggag tgccggattc atctatgtag caaagataca
     5521 tattaaacct ctaaaaaaaa agcgactggc aacagggcca tttaggcccc tttaggagac
     5581 gcttggcgac tccttccaga cgcatcaata acagtattct taaaattatg tatgtcaaat
     5641 ttttttatga aaatgagttt cttcagcata aagatttgtt gataaatcat tttttcaata
     5701 taaaatgcaa aaccagaacg gattttttga ggaaaaatga gtattttcaa acacatcaga
     5761 gaaatcaaac gaaacaaaag actttttgat gaaaaagtat atgaaatggt tttggatgaa
     5821 attgctcagg atcagaaaca gccaggatta tgggcaaaag caattgcaga ttcttcgggc
     5881 aacatggata aagcaaagtc tttgtacata aaatatagag ctgaagcaat aaagcaggac
     5941 attgattttc tcaaagacaa tgaattaaaa acagaaagga atgaaactca accgaaaaca
     6001 gagactaa
//
LOCUS       DAOOYB010000178         5993 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_15260_length_5993_cov_6.721623, whole genome shotgun sequence.
ACCESSION   DAOOYB010000178 DAOOYB010000000
VERSION     DAOOYB010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5993
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15260_length_5993_cov_6.721623"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(184..1182)
                     /locus_tag="PL056_10005"
     CDS             complement(184..1182)
                     /locus_tag="PL056_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47387.1"
                     /translation="MRKIFFLALIAASMVMGACVEQHNLVVLDKFVPISTQDQCQIKA
                     GGDRYYTKGYIDLAFTDQYMLPFQITNYISSSEVSDVGSGSAPISSAETNRFFVKWVE
                     VKYEWDPRPQSDGKQLSLTGDLWNKARRIEVHGVVADPEGGQNAGAVDIFTEDQAKDL
                     LDQLTNPEKDTTFDWVASPLLIKMKVVGEITDGSEIETNTLNFNIIPTFGESIQMGST
                     YREPAGGFADDQEKYDTIMAMCAFEDPLVGGCLIGQDSSMVNCYAGDTPWQKYIATSR
                     CHLDPTVTDCYYTGYGAASVVEVIYNRYKKGSTDSGYYYCCPGEEPEAPEEEETAE"
     gene            complement(1179..2174)
                     /locus_tag="PL056_10010"
     CDS             complement(1179..2174)
                     /locus_tag="PL056_10010"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase family protein"
                     /protein_id="HPM47388.1"
                     /translation="MEFDFSINYRTVISFRDDLADFTDEFGPESCFIVDKALKDLEKR
                     IPRERIFRLNSEKEKDLSHVENCIEWLISMNAGRNTKLVAVGGGAVTDFGAFTASVFN
                     RGMQLVLVPTTILAAVDSSIGGKTAVNFVAKNAIGTFYPANEVVVVKEFFKTLDKNMV
                     ASGKAEIVKIALIKSGKLFETVKSAGDLLSEESMKQAIKYKYDIIRYDLTDTLKKRMV
                     LNWGHTFGHAIERYYGIPHGLAVASGMVLMQKYAAWLGLDAYPAGELEELLNMNGINS
                     DVSRFLKEDKWRRFIVMDKKRILEDISMVYLKKVGSASIIKRNLNDILKDLEELK"
     gene            complement(2153..2656)
                     /locus_tag="PL056_10015"
     CDS             complement(2153..2656)
                     /locus_tag="PL056_10015"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HPM47389.1"
                     /translation="MKRIGFIGFSGSGKSSLTKLAQETGFKTVDTDSLMIERFGSNEF
                     FRIIDGDDAGFRSMESEVIKDALKSDAQIIAFGGGFHQGHKSFGNVMIPSVKLVYLRT
                     AFDDIVDRVKDRPLFIKLGIEKYRMLFEERAPLYAGCADFTVDAGERSLNGIWLEVER
                     IWNLIFR"
     gene            complement(2653..3687)
                     /locus_tag="PL056_10020"
     CDS             complement(2653..3687)
                     /locus_tag="PL056_10020"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HPM47390.1"
                     /translation="MQFVSAGESHGKALVAILSGFPAGLKFDRVLLETELIRRRSGSA
                     STKRLELETDEVKILSGISGDVTTGAPLSFLVENSPQGTQFFENRVKGKGFPRPGHVD
                     YCSYIKFGYDDFSIGSERSSARETVLRVAAGSFCKMLLKEFGMAVSSQILEIGGVEFN
                     SFDPEQQKNDIEKGSCGGVLKVILSGVPVGLGSNSQWFDKLDANLARAFISIPSVKGV
                     YFGETSVHKMRGVESIDHFTGNGAERKSNMAGGIEGGISNGCPVVATLYFKPVPTQPV
                     EFETVSPVDGEKGMCGGGMNDLWCIDRAAVIAESMLAFVAADAFCKKFGSDSMADIIS
                     SYESYCERSK"
     gene            complement(3697..>5993)
                     /gene="pilQ"
                     /locus_tag="PL056_10025"
     CDS             complement(3697..>5993)
                     /gene="pilQ"
                     /locus_tag="PL056_10025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HPM47391.1"
                     /translation="KIDGNSVDILGLGECRKGIEFNGIKNGSVNGKNASMINIPLVDG
                     SMVIEVNASGSKTVKTGDFETMVSLKEQKQLKESRVTMSKDPGSIKFAFSVQPQTVRF
                     FSLTGPDRLVFDFIGIKGNMVSTKDARSANHPSGFRVVVEKDIPQFFAVKKGSGFEFA
                     VDGRKLFSSFAPVTDNKVVQVPVQTAPVKEEKQEERVPSVEKTEITGLAFIGEDPEIL
                     KIKAEKQLNVSKKIVDSKIEITIEKAFIVKEKEQVIDASSLNGPVSEIAIFNDKDDVK
                     ILAKMKSAKFEIEIEDVSNGKEFVFQNKKEKTQDVAGYSEAQIVRVPQMTTGQYEEDP
                     LKSGGAVKMDEEVVQYTGKKINLDFKEVDILDILRLMSDISKLNIIAGDDVKGNVTVR
                     LVDIPWDEALEVILKSKSLGKERFGNIIRVATIKTMQREKEEELAKKNAQKKLEPVKV
                     RLIPVNYAMAVKLMPQIKELLTDRGTVSTDERTNVLIVKDVEEILDKSEQLVEYLDTQ
                     TPQVLIEARIVEATSTAALGLGIEWGTTHTYTDANGHPTGVVFPYNMGVGGAVSVPGP
                     ADRTGALGFSFGSVGMINDLNLSLNVMESEGKIKIVSSPKVATLDNTEAMIQQGVSIP
                     ITTRGTGGDVTTKYVDATLVLKTTPHITADGSILLSLEINKSEPDWSNANYLGEPSII
                     KKEAKTEILVKSGDTVVIGGVYTNKTSITKRGVPLLSQIPILGWLFKSEESRVERSEL
                     LIFLTPRIMNKVKSSIPLMKNEGE"
BASE COUNT         1624 a         1492 c         1027 g         1850 t
ORIGIN      
        1 gaaaaaatta taatgacgaa ccatctgtat tttacaacta attccgataa cttaaccatt
       61 acaagacctc aaaaacagaa tgatcacaat acaaaaattg gactacttta caacacacgc
      121 ggttacaaat tcaagatttt tgaagattat attagaaaaa ataaactctg tggactccgc
      181 cgattattcc gcagtctctt cttcttcagg agcttccggc tcttcacccg gacaacagta
      241 ataatatccg ctgtcggtag aacctttctt gtatctgtta taaatgactt caactacact
      301 tgccgcaccg taacctgtgt agtaacaatc cgtaactgtc gggtcaagat ggcatctgga
      361 tgtagcaata tacttctgcc acggagtgtc acctgcataa cagttcacca ttgaactgtc
      421 ctgaccgatc agacagccgc caactaaagg atcttcaaat gcacacattg ccataatggt
      481 gtcatatttt tcctgatcat cggcaaatcc gccagccggt tctctatatg ttgagcccat
      541 ctgaatggac tctccgaaag tcggaattat attaaagtta agggtgtttg tttcaatttc
      601 acttccatct gtgatctcgc ctacaacttt catttttatc agaagtgggc ttgctaccca
      661 gtcaaaggtt gtgtcttttt cagggtttgt caactgatct agtaaatctt ttgcctggtc
      721 ttctgtaaaa atgtcaacag ctcctgcatt ttgtccacct tcagggtctg cgacaacacc
      781 gtggacctct atccttcttg ccttgttcca gagatctcct gtcaaagaga gctgtttccc
      841 gtcagattga ggtcttggat cccattcata tttaacctca acccacttta caaaaaatct
      901 gttggtttcg gcacttgaga taggagcgct tcctgaaccg acatcgctaa cctctgaaga
      961 agagatgtag ttagttatct gaaaaggaag catatactga tcagtaaaag ccagatcgat
     1021 ataacctttt gtgtaataac ggtctccacc tgctttaatc tgacactgat cctgtgtgga
     1081 aataggcaca aatttgtcaa gaacgacaag attgtgctgt tcaacacacg cccccataac
     1141 catagatgcg gcaatcagag caagaaagaa aatttttctc attttaattc ctccaaatcc
     1201 ttcaatatgt cattcaaatt cctcttaatt atcgaggcac tacccacttt tttaagatag
     1261 accatcgaaa tatcctccaa aatccttttt ttatccatca caatgaatct cctccatttg
     1321 tcctctttta gaaaacggct gacatcactg ttaattccat tcatattcaa cagttcctca
     1381 agttctccgg caggatatgc atccaagccc agccaggcgg catatttctg cattagaacc
     1441 attccggatg ccacagcaag cccatgcgga atgccgtaat atctttcaat cgcgtgaccg
     1501 aaagtgtgtc cccagttcag aaccattctt ttcttaaggg tgtccgtaag atcatacctg
     1561 attatgtcat atttgtactt tatggcctgt ttcatgcttt cctctgaaag aagatcaccc
     1621 gcagatttaa ctgtttcaaa cagctttccg ctttttataa gtgcaatttt cactatctct
     1681 gcctttccgg atgcaaccat gttcttatca agtgtcttaa aaaactcttt tacaacgact
     1741 acttcatttg ccggataaaa tgtacctatg gcattctttg ccacaaaatt cacagccgtc
     1801 ttcccgccta tactgctgtc gacagcagca aggatcgttg tcggaacgag aacaagctgc
     1861 attcctctat taaaaacaga cgcggtaaat gctccaaaat cggtaacggc tcctccccct
     1921 acagcaacaa gctttgtatt ccgtccggca ttcatgctta taagccattc gatgcagttc
     1981 tctacatgac tgagatcttt ctccttttcg ctgtttaaac ggaatattct ctccctgggg
     2041 atcctttttt ccagatcttt aagagcttta tccactatga aacagctttc cggtccgaac
     2101 tcatccgtaa aatcggcaag atcatccctg aaactgatca ctgttctgta atttatcgaa
     2161 aaatcaaatt ccatattctc tccacttcga gccagatccc gttaagcgat ctttcaccgg
     2221 catcaactgt aaaatcggca caccctgcat aaagaggtgc cctttcttca aaaagcatcc
     2281 ggtatttttc aattccgagc ttaataaaga gcggacgatc tttaacacgg tcaactatat
     2341 cgtcgaaagc tgtcctcagg tatacaagct ttacactcgg aatcatcaca ttcccgaacg
     2401 acttgtgacc ttggtgaaag ccgcctccga aagcaattat ctgagcatcg gactttaaag
     2461 catcctttat cacttctgac tccattgatc tgaaaccggc atcatctcca tcaatgatac
     2521 ggaaaaattc attggaaccg aatctctcga tcatcaacga gtcagtgtca actgtcttaa
     2581 atcctgtttc ctgtgccaac tttgtcagag agcttttccc gcttcctgaa aaacctataa
     2641 acccgatccg tttcattttg atctctcaca gtagctttca tacgatgata taatatcagc
     2701 catgctgtct gaaccgaact ttttgcaaaa tgcgtcagca gcaacaaaag ccagcatact
     2761 ttcggctata acagcagccc tgtcaataca ccagagatcg ttcatacctc ctccacacat
     2821 ccccttttcc ccgtcaacag gagaaactgt ctcaaattca actggctgtg tcgggaccgg
     2881 tttgaaataa agtgttgcga caacaggaca tccatttgat atgccgcctt ctatgcctcc
     2941 tgccatattg ctttttcttt cggccccgtt ccctgtaaaa tgatcaatac tttcgacacc
     3001 tctcatttta tgaaccgagg tttcaccgaa gtaaacacct tttacagaag gaatcgatat
     3061 aaaagcgcgt gccagatttg catccagctt gtcaaaccac tgggaatttg atccaagtcc
     3121 aaccggaact ccggaaagaa tgactttcaa aacaccgccg cagcttcctt tttcaatgtc
     3181 atttttctgt tgttcagggt caaaagagtt aaactccaca ccccctatct caagtatctg
     3241 tgatgaaaca gccattccga attcctttaa aagcattttg cagaatgaac cggcagcaac
     3301 cctcagaaca gtttcccttg cgcttgacct ttccgaaccg attgaaaaat catcgtatcc
     3361 gaattttata tacgaacagt aatcaacatg tccaggtctc ggaaaccctt ttcctttaac
     3421 tctgttttca aagaactgtg tcccctgcgg actgttttcc acaagaaacg ataacggagc
     3481 tcctgtagtg acatccccgg aaattccaga aaggattttc acttcatcgg tttcaagctc
     3541 aagccgtttg gtgctggccg atcctgatcg cctccgtatc aattcagttt ccaaaagaac
     3601 cctatcaaac ttcaaacccg ccgggaaacc gctcaaaatt gcgacgagcg cttttccgtg
     3661 cgattcacct gcagacacaa actgcatttt aaagaactat tccccttcgt ttttcataag
     3721 cggtatcgag gacttaactt tattcatgat cctcggtgtg aggaaaatga gaagttcgct
     3781 tctttccact ctgctttctt cagacttgaa aagccatccg agtatcggta tctgagaaag
     3841 gagaggtacg cctctttttg ttattgatgt cttatttgta taaacaccgc cgataacaac
     3901 tgtgtcgccg ctttttacaa gtatttctgt ctttgcctct tttttgatta ttgacggctc
     3961 tccgaggtag tttgcatttg accagtccgg ctcactcttg tttatctcaa gactgagaag
     4021 tattgaacca tctgcagtaa tatgaggtgt tgttttaagc acaagtgtcg catcaacata
     4081 ttttgttgtc acatcaccac cggtacctct tgtagtaata ggaatactta caccctgctg
     4141 tatcattgcc tcggtattat caagtgtagc aactttagga gaacttacta ttttgatctt
     4201 accttccgac tccatgacat taagtgacag attaaggtca tttatcatac ctacactgcc
     4261 gaaagaaaaa ccgagagcac cagtcctgtc tgcaggtccc ggaactgaaa cagctccgcc
     4321 gactcccata ttatacggga acacaacccc tgtaggatgg ccgttcgcat cagtataagt
     4381 gtgtgtcgtg ccccattcaa tacccagtcc gagtgcagca gtagatgtag cttcaacaat
     4441 tctcgcctca ataagaacct gcggagtctg cgtgtcaaga tattcaacta gctgttcaga
     4501 tttatcaagg atctcttcca catctttaac aataagaaca ttcgttcttt catctgtgct
     4561 gacagtacct ctatcagtta aaagctcttt tatctgaggc ataagtttta cagccattgc
     4621 ataatttaca ggaataagtc ttacttttac cggctcaagt tttttctgag catttttctt
     4681 ggctagctcc tcttcctttt ctctctgcat tgtctttatt gttgcaactc tgattatgtt
     4741 accgaaccgc tccttaccga gagatttact cttaagaatt acttccagag cttcatccca
     4801 tgggatatca acaagtctta cagtgacatt tcctttaaca tcgtcaccgg caatgatatt
     4861 aagcttgctt atgtctgaca taagacggag aatatcaaga atatcaactt ctttgaaatc
     4921 gaggttgatc tttttccctg tatattgaac gacttcctca tccattttaa cagccccgcc
     4981 ggatttcaaa ggatcttctt cgtactgtcc tgttgtcatc tgcgggaccc ttactatctg
     5041 agcttctgaa tatcctgcta catcctgggt cttttctttt ttattctgga aaacaaattc
     5101 ctttccgtta gaaacatctt ctatttctat ctcaaatttt gcagacttca ttttggcgag
     5161 aatcttaaca tcgtctttat cgttgaatat agctatctca gaaacaggac cgtttaaaga
     5221 agaagcatca ataacctgtt ctttttcttt gacgatgaac gccttttcga tggttatttc
     5281 aattttgctg tcaacgatct tttttgagac attcagctgt ttctcggctt ttatcttaag
     5341 tatttcggga tcttcaccta taaaagcaag ccctgtgatc tctgtttttt caactgacgg
     5401 taccctctct tcctgttttt cttcttttac aggagccgtc tgaacaggaa cctgtactac
     5461 tttattatct gtaaccggag caaatgaact gaaaagcttt cttccgtcaa cagcaaactc
     5521 aaatcctgat ccttttttta ctgcaaagaa ttgagggatg tctttttcga caaccactct
     5581 aaaacctgaa ggatgatttg cacttctggc atccttagta gaaaccatgt ttcccttgat
     5641 acctataaag tcaaaaacca gtctgtcagg acctgtgagc gaaaaaaatc tgaccgtctg
     5701 cggctgaacc gagaaagcga attttattga accaggatcc tttgacattg tcacccggct
     5761 ctctttcagc tgtttctgct ctttaagaga caccatggtt tcaaaatcac ctgtctttac
     5821 ggtttttgaa ccggatgcat taacttcaat gaccatactt ccgtcaacaa gcggaatatt
     5881 gatcatggaa gcgttctttc cgttcacaga accgttttta attccgttga actcaatccc
     5941 cttcctgcac tctcccagtc cgagaatatc tactgaattt ccatcgatct tga
//
LOCUS       DAOOYB010000179         5979 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_15301_length_5979_cov_11.436361, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000179 DAOOYB010000000
VERSION     DAOOYB010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5979
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15301_length_5979_cov_11.436361"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(28..1788)
                     /locus_tag="PL056_10030"
     CDS             complement(28..1788)
                     /locus_tag="PL056_10030"
                     /inference="COORDINATES: protein motif:HMM:NF012664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47392.1"
                     /translation="MRKFLIIFAMFFLFISCEVEKRRALSDEDNINIILDEESSDSVN
                     DETFKEVDDPANDESEQQDDFEIKDEDNETVDEEIPVFPVEFESGFIEIEPANYTLDT
                     RDQTSARARMWYNFQPADESAHEKPLFVFFNGGPGAATALLFTYNTAKMTADQKYSES
                     DVVENEFNWNQIGNLLYIDARMTGFSYGMTGNPSSQNERSSYFRTNNFNVFIDAADFI
                     RVILRFLARHPDIQQNKVILVGESYGGTRATAMLNILLNVRDYADNNRVFHDKALFDE
                     IDAHFMKINPEISALPHKEIIKQQFAGQILVQPLVAGEEQFTASGKLLEMDGSPIYEV
                     ENETGVDYKPCSNSYCEPHNNALQYLQKADRDMYSYRRPYNWLFDYTATGSEKMVELD
                     LFEKLIKNDPREIEWLYSGNRSEAFRYSGLNYFKGLDFDMSRVPDSIKTEIEYRKTGM
                     KTLASGDLEQVFGILQSWDDYFIDLHYDVTQIFYNATTTPYSSINGYMFLENIRDVKT
                     FITQSEEDIIIYAPGIPESLKDFSGVNSVETEAEKFTVKFSDNVNAEVIFPFYPESSH
                     SVSVNQPEMFLNDVKEWLNF"
     gene            complement(1801..2832)
                     /locus_tag="PL056_10035"
     CDS             complement(1801..2832)
                     /locus_tag="PL056_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47393.1"
                     /translation="MQFLVKFKNRQLLYYYVLFLFIIGSCTYGRGGNDTPDDAEMEEI
                     DEEKEKDENIFPDKAVNDDTAEYDADTLDDDVSFDEFMDMADEFFFDNDDMTDEDISD
                     LPDLNGSWVKLMIFRGTAKPFLISKCNAWAIMIEKVTFTQKENVVESQNEMCRLKVGN
                     DTFPKIYSLVPDSYAQSLDIVEKTNYLSKNEKGEIFFHQPKVWDVRACTLIDPENDPL
                     PTDVNSENVFDPDNTGINGLLIYAKGTVNGEAEIVQKVSTILNGKLDENGEIRGLTTW
                     SEDQKVLATNNDLLWKGAPTSPDPGIDESESYFIFKRIAPELDCDWIRENSAVLFEEA
                     AKVYPDEFK"
     gene            complement(2833..3828)
                     /locus_tag="PL056_10040"
     CDS             complement(2833..3828)
                     /locus_tag="PL056_10040"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HPM47394.1"
                     /translation="MAKVKSLIKALKRKKRVFIQTHNYPDHDSVASAFALQYLLKQHD
                     IESDIIYHGEILRDSLQRMIDRLGIDIRHHSQYELDESDIIVIVDANKKSKNVAELKG
                     KVVAVIDHHQGTAYDDLDFCDIRSDYGACATIVFSYFADLRLEPSVEVATAIHTAINF
                     DTHQFTRNVNSLDIETVALLYRVSDVALVNSLVRNSIKVDDLSKYSYLLENVKFHHNF
                     AYCHFPNGCDKNLLAILSDYLLTIDEVSFAVLSAKYLDEIIFSVRSELAELPAHIMIS
                     EVLNGIGSGGGHIDMAGGRMPVSVDFKEEDIYNKFLSQVRLRNSFRITISDSWGG"
     gene            complement(3831..5087)
                     /locus_tag="PL056_10045"
     CDS             complement(3831..5087)
                     /locus_tag="PL056_10045"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HPM47395.1"
                     /translation="MEPLFGQFLPENIGQVISENLLLIVLFDMAVVSLISFISWFAAR
                     KFVNIFIKFAVERSKNSWDDVFYKNKVFTKLANIAPAIVIYLFSHQFTGEHILLKKLL
                     ITYVVIVLLAVFRSAGNAFLEIYETFEISKDRPLKGIVQILNIIAYVVGFIVVIAIMA
                     DKSPAILLSGLGAMTAIFMLVFRDTILGFVAGIQIVSNNSIRKGDWIVMNKYEADGEV
                     IDIALHSVKVQNWDRTIVSIPTHKFLEESFTNWRGMVESGARRISRSIFIDMSSIYFL
                     SESEIKEFRKIQLISEYLEKRTAEIKKWNEENHTDTAMPVNGRRLTNVGTFREYARQY
                     LLNHNGLRKDMTLLVRQLAPGETGLPLQIYVFAKTTVWSEYESIQSDIFDHLLAAVEY
                     FGLRVFQNPSGNDFRSLSQSLNRPGN"
     gene            complement(5095..5529)
                     /locus_tag="PL056_10050"
     CDS             complement(5095..5529)
                     /locus_tag="PL056_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit C"
                     /protein_id="HPM47396.1"
                     /translation="MDFGSLGPIMCLGLAAAGSAIGCGVAGMTSHGVMSKVDEGHAKF
                     VALSAMPASQSIYGFALMIILNGKLQELISAGKDTFSVFAIGLATGLAFMLSAIYQGK
                     ACSSAILAVAKKPEVFGMSFAAPGIVESFALFALVGGIMMAA"
     gene            complement(5570..>5979)
                     /locus_tag="PL056_10055"
     CDS             complement(5570..>5979)
                     /locus_tag="PL056_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47397.1"
                     /translation="ETLNLKAYEYAECYTGELTTDHGAFYLSCDGYEYRFEGRPDTYF
                     NFYQLDGDIREMQENHVYNGWTYIPDGSNVKLSTLNDVPSYCGNGVIEEGELCDGNSV
                     ECTTISSSYVSGTAYCNSVCNGYDETNCGEDGW"
BASE COUNT         1732 a         1329 c         1074 g         1844 t
ORIGIN      
        1 gaatattaaa tcaaaagatt ttttttgtca aaagttgagc cattccttaa catcgtttaa
       61 aaacatttcg ggctgattta cagaaacact gtgggaactt tcaggataga aaggaaatat
      121 tacttcggca tttacattat cagagaattt tactgtgaat ttttcagctt ctgtttcaac
      181 gctgttcacg ccgctgaaat ccttcagtga ctcaggaatt ccgggagcat atatgataat
      241 atcctcttcc gactgagtaa tgaatgtctt aacatcgcga atattttcaa ggaacatata
      301 tccatttatt gagctgtagg gtgttgttgt cgcattataa aagatctgtg tcacatcata
      361 atgaagatca atgaaataat catcccagct ctgaagaatt ccaaaaacct gctcaagatc
      421 accggaagca agggttttca ttcctgtttt acggtattca atctcggttt ttattgaatc
      481 gggaaccctg ctcatgtcaa aatccaggcc cttaaaatag tttaaacccg aatatctgaa
      541 tgcttctgaa cggttgcctg aataaagcca ttctatttcc cttggatcgt tttttataag
      601 tttttcaaaa agatcaagtt caaccatttt ttccgatcct gtcgcagtgt aatcaaaaag
      661 ccagttatac ggccttctgt atgaatacat gtctctgtct gctttctgaa gatactgcag
      721 agcgttgtta tgcggttcac aatatgaatt tgaacacggt ttgtaatcta cacctgtttc
      781 attttcaact tcatagatcg ggcttccatc catttcaaga agttttccag aggcagtgaa
      841 ctgctcttct cccgccacaa ggggctgaac aagtatctgg cccgcaaact gctgttttat
      901 tatttctttg tgcggaagtg ccgaaatttc aggatttatc ttcataaaat gggcatctat
      961 ttcatcaaaa agagctttat catggaaaac tctgttgttg tcagcataat ctctcacatt
     1021 aagaagaatg tttagcatcg ccgtcgccct tgttccaccg taactttcac cgacaagaat
     1081 gactttgttt tgctgaatat cgggatgtct cgcaagaaac ctcaggataa cccttatgaa
     1141 atctgccgca tcaataaaga cattgaagtt atttgtcctg aaataactgc ttctttcgtt
     1201 ctgacttgaa ggattaccgg tcataccgta agaaaatccg gtcatccttg catcaatata
     1261 aagaagattc cctatctgat tccagttaaa ttcattttcc acaacatcag attcagaata
     1321 tttctgatcc gccgtcattt ttgcagtgtt atatgtgaaa agaagggctg ttgccgctcc
     1381 gggaccgcca ttaaagaaca caaaaagcgg tttttcatga gccgattcat ctgccggctg
     1441 aaagttatac cacattcttg ctctggctga ggtctgatct cttgtatcaa gagtgtaatt
     1501 tgccggttcg atctcaatga aacctgattc aaattccact ggaaaaacag ggatctcttc
     1561 atcaacggtt tcattatctt catccttgat ctcaaaatca tcctgctgtt cactttcatc
     1621 attggcagga tcgtcaactt ctttaaaagt ttcatcattg acactgtctg aagattcctc
     1681 atccaaaata atgttgatgt tgtcttcatc agacaacgct cttctttttt caacttcgca
     1741 ggagatgaaa agaaagaaca tcgcaaatat tataagaaac tttctcatat cgcactactc
     1801 ctatttaaat tcatccggat acacttttgc cgcttcctca aaaaggactg cactgttttc
     1861 ccttatccag tcacaatcaa gttcaggagc tatccgctta aaaataaaat aactctctga
     1921 ctcatcaatt ccaggatcag gagatgtcgg ggcacctttc catagcaaat cgttgtttgt
     1981 agcaagaact ttctggtctt cagaccatgt tgtcagccct ctgatttcgc cgttttcgtc
     2041 aagttttcca ttgaggatcg tgctaacttt ctgaacaatt tccgcctcac cgtttacagt
     2101 tccttttgca taaataagca aaccatttat tccagtatta tccggatcaa aaacattttc
     2161 tgaattgaca tctgtaggaa gtggatcgtt ttcgggatca attaatgtac aagccctaac
     2221 atcccatact ttgggttgat ggaaaaatat ttctcctttt tcattttttg aaagataatt
     2281 agttttttcc acaatatcaa gagattgagc atagctatca ggaaccagcg aatatatttt
     2341 gggaaaagta tcattgccaa cttttagccg gcacatttcg ttttgagatt caacgacatt
     2401 ttctttctga gtaaatgtca ctttttcaat cattattgcc caggcattgc attttgaaat
     2461 cagaaacggt ttggctgttc ctctgaagat cataagcttc acccagcttc cgttaaggtc
     2521 gggaagatct gaaatatctt catcggtcat gtcgtcatta tcaaaaaaga attcatcggc
     2581 catatccata aattcatcaa atgacacatc atcatcaagc gtgtcagcat catattcggc
     2641 agtatcgtca ttaacagctt tatccggaaa aatgttctca tctttctctt tttcttcgtc
     2701 gatctcttcc atttcggcgt catcaggggt gtcgttgcca cctcttccgt aagtgcatga
     2761 cccgatgatg aaaaggaaca gaacataata ataaagcaac tgcctgttct taaatttgac
     2821 taaaaactgc atctatccac cccaactgtc cgatattgtt atcctgaaac tgtttctaag
     2881 ccgcacctga cttaagaatt tattatagat gtcctcttct ttgaagtcaa cacttaccgg
     2941 catccttcct ccagccatat cgatatgccc gccgcctgaa ccgattccgt tcagaacttc
     3001 agagatcata atatgcgccg gaagttcagc cagttcgctt ctgacagaaa agattatttc
     3061 atcaagatat ttcgcactca gtacggcaaa tgaaacttcg tcgattgtca aaagatagtc
     3121 tgaaagtatt gcaagcaggt tcttgtcaca gccgtttgga aaatggcagt atgcaaaatt
     3181 gtgatgaaat ttaacatttt caagcagata actgtatttt gaaagatcat cgaccttgat
     3241 tgagttcctg accagagaat tcacaagggc gacatcgctc actctatata aaagcgcaac
     3301 agtttcaata tcaagagagt tgacattcct tgtgaactga tgtgtgtcaa agtttatggc
     3361 agtatgaatt gccgttgcaa cttcaacaga cggttcaagg cggagatctg caaaataaga
     3421 aaagacgatc gttgcacagg caccgtaatc acttctgata tcgcagaaat cgaggtcatc
     3481 gtaagcagtc ccctgatggt ggtctatcac tgcgacaact ttccctttca gttcagcgac
     3541 atttttgctt ttcttgtttg catcaacgat gactattata tcactttcat caagttcgta
     3601 ctgactgtga tgtctgatgt caataccgag ccggtctatc attctctgca gagaatctct
     3661 aagaatttca ccatgataga taatatccga ttcaatgtca tgctgtttta gaagatattg
     3721 cagagcaaag gcgctggcaa ctgaatcatg atcaggatag ttgtgcgtct gaataaaaac
     3781 tctttttttc cttttcaatg cctttatgag tgacttaact tttgccattt tcagttaccc
     3841 ggtctgttta gagattgaga tagagacctg aaatcatttc cgcttggatt ctggaaaact
     3901 cgaagtccga aatattctac tgctgctaaa agatgatcaa aaatatctga ctgtatcgac
     3961 tcatattcag accatacggt cgttttggca aagacatata tctgaagagg aagtcctgtc
     4021 tctcccggtg caagctgtct tacaaggagt gtcatatctt ttctgagtcc gttgtgattt
     4081 aaaagatact gtcttgcata ttccctgaaa gtaccgacat ttgtaagtct tctcccgttc
     4141 acaggcatcg ctgtgtcggt gtgattctct tcattccact tctttatttc cgccgtcctt
     4201 ttttcaagat attcagatat aagctggatc tttcgaaact cttttatttc agattcactt
     4261 aaaaaataaa tgcttgacat atcaatgaaa atactgcggc ttatccttct tgcaccggat
     4321 tcgaccatcc cccgccagtt cgtaaaactt tcttcaagga atttgtgggt cggaatgctc
     4381 acaatggtcc tgtcccagtt ctgaaccttg acagaatgaa gagctatgtc aatgacttct
     4441 ccatccgcct catatttgtt cattactatc cagtcgcctt ttcttatcga gttgttggaa
     4501 acgatctgaa tacccgcaac aaagccgaga atggtgtctc tgaaaaccag catgaatatt
     4561 gccgtcatag ctccgagacc gcttaaaaga attgcggggg atttatccgc cattatcgca
     4621 attaccacta tgaaaccgac tacataggca attatattca atatctgaac gatcccttta
     4681 agcggtctgt cctttgatat ctcaaaagtt tcatatatct caaggaaagc atttccggca
     4741 gaccggaaca cagcaagaag cactatcaca acataagtaa taagcagttt tttcaagaga
     4801 atatgttcac ccgtaaattg atgggaaaaa agatatatca caattgccgg agctatgttt
     4861 gcaagcttgg tgaacacttt gttcttataa aaaacatcat cccatgaatt ttttgatctc
     4921 tcaactgcaa actttataaa aatatttaca aatttccttg ccgcaaacca cgaaatgaaa
     4981 gatatcagag aaactacagc catatcaaac agaactataa gcagcaaatt ttcagaaata
     5041 acctgtccga tgttttcagg gagaaactgt ccgaaaagag gttccatatt gatcttatgc
     5101 cgccatcatt attccgccga caagagcgaa aagtgcgaat gattcaacaa taccgggagc
     5161 cgcaaagctc attccgaaaa cctcgggttt ctttgcaact gcaagaatcg cacttgaaca
     5221 tgccttgccc tgataaatgg cactgagcat gaaagcaagt ccggttgcaa gaccgatcgc
     5281 aaacactgag aaagtatcct tgccggcaga aatgagttcc tgaagttttc cattcaggat
     5341 tatcataagt gcaaaaccat agatcgactg agacgcaggc atcgcagaaa gagctacgaa
     5401 ctttgcatga ccttcatcaa cctttgacat aactccgtga gatgtcatac ctgcaacacc
     5461 gcatccgatt gcactgcccg cagcagcaag accaagacac ataatcgggc ctaaacttcc
     5521 aaaatccatc tttctctcct ttaaaaaaag ttattaattc tcgccgttac taccacccat
     5581 cttctccaca attcgtttca tcatatccat tgcagactga attacaatat gccgtgccgc
     5641 tcacataaga tgaacttatt gttgtacatt caactgaatt tccgtcacag agttctcctt
     5701 cctctattac tccgtttccg caatagctcg ggacatcgtt gagggttgag agttttacat
     5761 tgcttccgtc agggatgtat gtccagccgt tataaacatg gttctcctgc atttcacgga
     5821 tgtcaccgtc aagctgatag aaattaaaat atgtatccgg tcttccctca aaacggtatt
     5881 catacccgtc gcaggagaga tagaaagctc cgtgatctgt ggtaagttcg cctgtgtagc
     5941 attctgcgta ttcatacgct ttaagattaa gtgtctcaa
//
LOCUS       DAOOYB010000180         5863 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_15712_length_5863_cov_9.377927, whole genome shotgun sequence.
ACCESSION   DAOOYB010000180 DAOOYB010000000
VERSION     DAOOYB010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5863
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15712_length_5863_cov_9.377927"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..374)
                     /locus_tag="PL056_10060"
     CDS             complement(84..374)
                     /locus_tag="PL056_10060"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012072576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HPM47398.1"
                     /translation="MIDLSPKHLETVREILKYHLPVCEVRVFGSRIKGTAKSYSDLDI
                     ALVCKEKIQRKKLHEIREAFEESDLPIRIDIIDFNAISKEFQEVICQKYEVL"
     gene            complement(367..789)
                     /locus_tag="PL056_10065"
     CDS             complement(367..789)
                     /locus_tag="PL056_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase substrate binding
                     protein"
                     /protein_id="HPM47399.1"
                     /translation="MKLVIDSLKSALISLEKAVDISIQKEKDKSVSQDELDVIQAGVI
                     QNFEFTYELCWKFMKRWIEVNIGATYVDGVTRIELFRQASENRLISDVEKWVGFHRAR
                     NETSHIYDEEIAEFVYETAKVFLDDAKKFLKALEERND"
     gene            complement(786..2261)
                     /gene="glgA"
                     /locus_tag="PL056_10070"
     CDS             complement(786..2261)
                     /gene="glgA"
                     /locus_tag="PL056_10070"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HPM47400.1"
                     /translation="MKIAFIASECEPFVKTGGLADVVSSLPKALKELGHEVMIILPYY
                     GAVKRKNINTVTFFDSMGVWMGAGIQEWCSVRKTIAEDEVPVFFIEFDHYFDRSGLYC
                     DEINRDYTDNAARFAFFSRAALQLLKDMDFQPDVVHAHDWQTAPVVAYMKTWGWGKSG
                     IDKAAAVLTIHNIGYQGVYHADAYKYCGFSEWDFVPDIFEDHGRINFLKGGIHYADMV
                     TTVSPTYAKETTIGEQSYGFAPYLNNKGERYKGILNGVDYSSWDPSNDKLIPAKFSAD
                     KMEGKHECRKILKQKFGLDDSKDVPVLGVVSRFADQKGLDVFAAAVEKILNTMEIHIA
                     ILGSGDKALESYFGWLPSMMPGKAGAVIGYNNEIAHLIEAGSDFFIMPSRYEPCGLNQ
                     IYSLRYGTLPIVRNTGGLADTVIQYDERTGDGTGFKFDYLSVDSIFDTVGWAVSTYFD
                     RPQHLKKMRKTAMNQDFSWTKSANEYEGIYDEILTEKRGEK"
     gene            complement(2280..3380)
                     /gene="tgt"
                     /locus_tag="PL056_10075"
     CDS             complement(2280..3380)
                     /gene="tgt"
                     /locus_tag="PL056_10075"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013011715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HPM47401.1"
                     /translation="MISFKIGKTSGKARSGIISTERGEIKTPIFMPVGTQGTVKAVLP
                     DQLEELGAQIILGNTYHLFLRPGHELIKQTFGSLHKMMNWNRPILTDSGGFQVFSLGP
                     LRKIKEEGVYFSSHLDGSKRFISPEISIEIQESLGSDIMMAFDECPALPCTRDYMLDS
                     MDRTTRWAKRCLEARTTDAALFGIFQGGVDIFLRKKHLEEISALPFDGFAIGGLSVGE
                     EKEDMYKIIEEIGHLMPEDKPRYLMGVGTPRDIVKAILEGIDMFDCVIPTRNARNGQL
                     FTWSGTLNIKRQEFERDTLPIDEKCDCYTCRNFSRSYLRHLYKAGEYLSPILNSIHNL
                     HFYLELVKTARIHIENDTIEEFYKEISKVYER"
     gene            complement(3387..>5863)
                     /locus_tag="PL056_10080"
     CDS             complement(3387..>5863)
                     /locus_tag="PL056_10080"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPM47402.1"
                     /translation="GEAGIGKSRLIYEVKRKAGGDFQILSLSTDTILRQSMNMFTSFF
                     QRFFDQNENVSADENKLNFEKIWSSVSADVIGRKNEFEAAKPYIGALAGVFWKGSVYE
                     ILDAKSRYENTIFAVADFFVSLAERKPLMIVLDDMHAVDSDSLEVVKNMVKRLADFPV
                     VIYQLSRLNDDGSKPVILGITADNFKEMVIDKLLESDAADFIQNILNDRISHDLNRFV
                     FSRSEGNPFFMEQLVLFMNERGYILKTDEGFSLKMDDIEVPGEVNSLLVSRLDRLSLK
                     LKDLVFRASVLGNDIDLAVLSEITEEQEIVDTLKEGIDELVWNEVSKLVYSFRHALLR
                     DAAYSMQLKARLKEIHRKVAYAIETNYLNEGRFFSSLAFHFEKSEDIEKEIFYLRKAA
                     DYSKSQYHNTDAEKMYEKLMKMLTNNEELIEVGHLLGSVYKISGKWDLAEKTFRDSLE
                     TARNTGSDNLTASSMKELAYMLLEKGRYDEAQPLLDSAVELYQKLGAVKGEGDVEGYR
                     GLIHYYKGELDDAIEHFSRKLELAEKIDDKAAKALSYRYLGGVSYYRSNYKTALEYYS
                     SQLAISKETENMLDIAVAENNLGLVYSHMNDFTNAARFYRNALDTYKKIGIRQYICYT
                     SNNLGELKFWLGEYEEAKKLFEDQLTIATELGIKRHVSIAYNCLGNICRKQDSFEQAE
                     EYYNKAILIGEELNVQTILCEYYYEKASLFFEMKRFDESLELAEKAVKMSTEVNRQDF
                     RFRSELLKWRIVSISEPQTAIENIQRMIVENIKEENLAEVEFLLFILTKDEKHYEKAL
                     SLFEALYKLAPKAAYKERIESLLSEK"
BASE COUNT         1597 a         1340 c         1038 g         1888 t
ORIGIN      
        1 aaatagaagt taaaacaaaa actcgttagt ttgtcacacc cattttattt gtcagtcttt
       61 tccagtcttt tatggatagt tccttacaaa acctcgtatt tctggcagat aacttcctga
      121 aattctttgg atattgcatt gaaatcaata atatcaattc tgattggaag atctgattct
      181 tcaaatgctt ctcttatttc atgcagtttt tttctttgaa tcttttcttt gcagacaagc
      241 gctatatcaa ggtcggaata gctttttgca gttcctttga ttcttgaccc gaaaacccgc
      301 acttcgcaga caggcaggtg atatttaaga atttccctta ctgtttcaag atgtttcggt
      361 gaaagatcaa tcatttcttt cctctaaagc ttttaagaac ttttttgcat catcaagaaa
      421 aacttttgcg gtttcataaa caaattcagc tatttcctca tcataaatat gtgatgtttc
      481 attccttgcc ctatgaaatc ccacccattt ttcgacatca cttatcagcc ggttttcaga
      541 tgcctgtctg aaaagctcaa ttctcgtaac accgtcaaca taagtcgcac cgatattaac
      601 ttctatccac cgtttcataa atttccagca aagttcataa gtaaattcaa aattctggat
      661 cactccagcc tgaatcacat caagttcatc ctgagaaacc gatttatcct tctctttctg
      721 aatagatata tcaaccgctt tttcaagtga tatcaaagca ctttttaaac tgtcgatcac
      781 cagtttcatt tttcacctct tttttcagtt aaaatttcat catatatccc ctcatattca
      841 ttagcagatt ttgtccagct gaaatcctga ttcattgcgg tttttctcat ttttttcagg
      901 tgttgcggtc tatcaaaata tgtgctgaca gcccagccga ctgtgtcaaa tatcgaatca
      961 accgaaagat aatcgaactt aaagccggtt ccatcacctg ttctttcatc atactggata
     1021 actgtgtcgg caagtccgcc ggtgttcctg acgataggaa gagtgccgta tctgagtgaa
     1081 tatatctgat taagtccgca tggttcatat cttgacggca tgatgaaaaa atcgctcccg
     1141 gcttcaatta agtgtgcaat ttcattgtta tatccgataa cagctcctgc ttttccaggc
     1201 atcattgaag ggagccagcc gaaatacgac tcaagggctt tgtctcctga accgagaatg
     1261 gcaatatgaa tttccattgt atttagtatc ttttcaaccg ctgcggcaaa aacatcaagc
     1321 cctttctgat ctgcaaaacg gcttactacg ccgagtaccg gaacatcctt tgaatcgtca
     1381 agtccgaatt tctgttttag aatttttctg cattcatgct ttccttccat tttatcagca
     1441 gaaaacttag ccgggatcag tttgtcattg ctggggtccc agcttgaata atcaactccg
     1501 ttgagaatcc ctttatatct ttcacctttg ttgttaagat atggtgcaaa tccgtaactt
     1561 tgttcaccta ttgtcgtttc tttggcatag gtcggactta ctgttgtcac catatcggca
     1621 tagtggatcc ccccttttaa aaaattgatc cttccatgat cttcaaatat gtcaggaaca
     1681 aaatcccatt cactgaatcc gcagtattta taagcatctg cgtgatatac accctgataa
     1741 ccgatgttat gaatggtaag aaccgccgcc gctttatcaa ttccgctttt tccccatccc
     1801 catgttttca tatatgcgac taccggagcg gtctgccagt catgggcatg aaccacatct
     1861 ggctgaaaat ccatatcttt cagaagctgt aaagccgccc tgctgaaaaa agcgaacctt
     1921 gccgcattat cggtataatc tctgttaatt tcatcgcagt aaagcccgga acgatcgaaa
     1981 taatgatcaa attcaataaa aaatacaggt acttcatctt cagcaatagt ttttctgact
     2041 gaacaccact cctgaattcc cgcacccatc cagacaccca ttgaatcaaa aaaagtgaca
     2101 gtgttaatgt tttttctttt tacagccccg taataaggaa gaattatcat gacctcatgc
     2161 cccagctctt tcaaagcttt gggaagagag ctcacaacat cggcaagtcc gccggtcttc
     2221 acaaacggct cacattcaga agcaataaaa gcgattttca ttgacaactc ctgtattatt
     2281 tatctttcat aaacctttga tatttcttta taaaactctt cgatggtgtc attttcaata
     2341 tgtattcggg cggttttgac aagttcgaga taaaagtgaa gattgtggat ggaattgagt
     2401 atgggagata gatattcccc tgctttgtag aggtgtcgaa gataagagcg gctgaaattg
     2461 cggcatgtgt agcagtcgca tttttcatca atcggcaaag tatctctttc aaattcctgt
     2521 ctttttatgt tcaaagtgcc tgaccatgta aaaagctgac cgtttcttgc attccttgtc
     2581 ggtatgacac agtcaaacat gtcaattcct tctaaaatag cttttacaat atctctcggc
     2641 gttcccacac ccataagata cctcggttta tcttcaggca taagatgccc aatttcttca
     2701 ataattttat acatatcctc tttttcttca ccgacactca aaccacctat cgcaaatccg
     2761 tcaaaaggaa gtgccgatat ttcttcaaga tgttttttcc ttaaaaaaat gtcaactcca
     2821 ccctgaaaaa ttccgaaaag tgctgcatct gtcgttctgg cttcaagaca tcttttcgcc
     2881 caccgggtag ttctgtccat cgaatcgagc atataatcac gagtgcaggg aagagccgga
     2941 cactcatcaa atgccatcat tatatcactt ccgagcgatt cctgaatttc aattgatatt
     3001 tcaggggaaa tgaatctttt tgagccgtca agatggcttg aaaaataaac cccttcctct
     3061 tttattttac gcagaggacc taagctgaaa acctgaaaac cgccactgtc agtgagaatc
     3121 ggtctattcc agttcatcat tttatgcagt gacccgaaag tttgtttgat aagttcatgt
     3181 ccgggacgga gaaaaaggtg gtaagtattt ccaagaatga tctgtgctcc aagctcttca
     3241 agctgatcag gaagaacggc tttaactgtt ccctgagttc cgacaggcat gaatatcgga
     3301 gtttttatct ctcctctttc tgttgaaata atgcccgacc ttgctttgcc tgatgttttt
     3361 ccgattttaa aagagatcat ttgaccctat ttttctgaaa gtagtgattc gatccgctct
     3421 ttataggccg ctttgggtgc cagtttataa agtgcttcaa aaagagacag cgctttttca
     3481 taatgttttt cgtcttttgt aagtatgaaa aggagaaatt ccacttcagc cagattttct
     3541 tccttaatgt tttcaacgat catccgctga atattctcaa ttgccgtttg aggttccgat
     3601 attgaaacta ttctccattt gagcagttcg cttctgaacc tgaaatcctg ccggttgacc
     3661 tctgtgctca ttttaactgc cttttcagca agctccaagg attcatcaaa ccgtttcatt
     3721 tcaaagaaaa gtgatgcttt ttcgtaataa tattcacaca gtatggtttg aacattaagt
     3781 tcttcaccga ttaggatcgc cttgttgtaa tactcttccg cctgttcgaa tgaatcctgc
     3841 tttctacaga tgttgccaag acagttgtag gcaatggaaa catgcctttt tatcccaagt
     3901 tcggtcgcta ttgtaagttg atcttcaaaa agcttttttg cttcttcgta ttcaccaagc
     3961 cagaatttca actcaccgag attattggaa gtatagcaga tatactgcct tatcccgatc
     4021 tttttatatg tgtccagagc gttcctgtag aatcttgccg cattggtgaa atcattcatg
     4081 tgggaataaa ccagtccgag attgttctca gcaacagcta tgtcaagcat gttttctgtc
     4141 tctttcgaaa ttgcaagctg tgagctgtag tattcaagag cggttttgta attgcttctg
     4201 tagtaggaaa cacctcccag atatctgtag gaaagtgctt tagccgcttt atcatcaatc
     4261 ttttcagcaa gttccagctt tcttgaaaaa tgttctatcg catcatcaag ctcacctttg
     4321 taatagtgga taagccctct gtaaccttcc acatcacctt cacctttgac tgctccgagt
     4381 ttctgataaa gctcgacagc actgtcaaga aggggctgtg cctcatcata tctccctttt
     4441 tcaagaagca tgtatgccag ttccttcata cttgatgcag taagattatc actgccggta
     4501 ttccttgcag tttcaagcga gtccctgaat gtcttttccg caaggtccca ttttcctgat
     4561 attttgtaaa cgcttcccag aagatgaccc acctctatca gctcttcatt attagtgagc
     4621 attttcataa gcttttcata cattttttcg gcatctgtat tgtgatattg cgattttgaa
     4681 taatcggccg cctttctaag ataaaagatc tctttttcta tgtcttcgga tttttcaaag
     4741 tggaacgcca atgatgaaaa aaaccttccc tcgttcagat aattggtttc aatggcatat
     4801 gcgactttgc ggtggatctc cttgagacgg gccttcaact gcattgaata ggcggcatct
     4861 cttaaaagtg cgtgtctgaa tgaatagacc agctttgaaa cctcgttcca gaccagctca
     4921 tcaattcctt ccttgagagt gtccactatc tcttgttcct cggttatttc gctaagcact
     4981 gcaagatcta tgtcgttccc gagcactgat gccctgaaaa caaggtcctt gagcttaagt
     5041 gaaagccggt caagacggct tacaagcagc gaattgactt ctccgggaac ttctatgtcg
     5101 tccattttaa gagaaaaccc ttcatcagtt ttcagaatgt aacctctttc gttcatgaaa
     5161 agaacaagct gttccatgaa aaaagggttc ccttcgcttc ttgaaaaaac gaaccggttc
     5221 aaatcatggc tgatcctgtc attcagaata ttctgaataa aatcagccgc atccgattca
     5281 agaagcttgt ctatgaccat ctctttgaaa ttatctgcgg taatgccgag tattacaggt
     5341 ttagagccat catcgttaag ccgtgagagc tgatatatca caacaggaaa atcggcaagt
     5401 ctcttgacca tgttcttaac gacttcaagc gaatcactgt caaccgcatg catgtcatca
     5461 agaacgatca tcagcggttt tctttctgca agagagacaa aaaaatctgc aacagcgaaa
     5521 atcgtgttct catatctgct tttcgcatcc agtatctcgt aaacggatcc tttccagaaa
     5581 acaccggcaa gagctccgat ataaggtttt gccgcttcaa actcgttttt cctgcctatc
     5641 acatcggcag agacagaact ccatatcttc tcgaaattca acttgttctc atcagcagaa
     5701 acattttcat tctgatcaaa gaatctctgg aagaacgaag tgaacatgtt cattgactgc
     5761 cgcagtatcg tgtcagtgct caaactcagg atctgaaaat ccccgcctgc ttttcttttt
     5821 acttcataga taagtcgaga ttttccgatg cctgcttcac cga
//
LOCUS       DAOOYB010000181         5855 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_15742_length_5855_cov_8.345862, whole genome shotgun sequence.
ACCESSION   DAOOYB010000181 DAOOYB010000000
VERSION     DAOOYB010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5855
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15742_length_5855_cov_8.345862"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="PL056_10085"
     CDS             complement(<1..491)
                     /locus_tag="PL056_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47403.1"
                     /translation="MITGMQKLLLVGPAENKEKVLSALQRSGVVEVESYKGSLFPVDG
                     REVSTVRADLIIWAIKTVERYAAAAERTGLTRNDCEGDPATLVDRIPVLDREHRKCRE
                     ETVQLQNKLNFIAPWGDFLIKDIREIEQKGNVFIQLWDVAQKVAHEVKIEEAIAEIEI
                     FHD"
     gene            complement(491..1117)
                     /locus_tag="PL056_10090"
     CDS             complement(491..1117)
                     /locus_tag="PL056_10090"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF002565.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HPM47404.1"
                     /translation="MSAIKLNKTELKIQKNLLAQLTKYLPTLQLKKQQLQAEVENTKN
                     IESSILAEIKRRKDYVSNWAVVLSQKLTINVYQMVEIEEIVTDTENIAGVDVRVFKDL
                     KFKPFSYSFFIKPVWVDRAYEELRAIVVEREKLKIIRERMKLLSEELRQTSIKVNLFE
                     KRMIPECRENIRKIKIFLGDQEVAAICNAKIAKGKLKKKDEMQFAEAV"
     gene            complement(1117..2421)
                     /locus_tag="PL056_10095"
     CDS             complement(1117..2421)
                     /locus_tag="PL056_10095"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HPM47405.1"
                     /translation="MVKTYNRIERIKGSIAQVKAEGVMLGELARIKMSDGRETLGEVI
                     SFDHDRVNLQVYLGTKGVSTGDQVRFLGKPMQVGCGESMLGRVFNGSGEPIDNGPELM
                     EEKVDIGGPSFNPSKRIVPKRFVKTNIPMIDMFNALVISQKIPIFSIPGEPYNELLAR
                     IASQTDADIIILGGMGLKFDDYQFFKDYFTKTGAMEKTSMFIHLASDPVVECILVPDM
                     ALAAAEKFAIQNKDVLVLLTDMTAFSDSLKEISISMDMVPSNRGYPGSLYSDLAARYE
                     KAVDIESSGSITIIAVTTMPGNDVTHPVPDNTGYITEGQFYLHGGRIDPFGSLSRLKQ
                     QVMGKVTREDHGDLANAMIRLYADAKKARDRESMGFRLSGWDKKLLSYAYLFEEKMMD
                     LRVNLDIEEQLNLGWQILAGCFDRNETGLKNKWIEKYGKWDK"
     gene            complement(2423..4183)
                     /locus_tag="PL056_10100"
     CDS             complement(2423..4183)
                     /locus_tag="PL056_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006068411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HPM47406.1"
                     /translation="MVLQVEKTKGKIVAAQGNLMTVEFDGNVRQNEVVFVKTGGQSLK
                     GEVIEINGNQAKVQVFEPTKGVRLNDEVVFEHDLLSVELGPGLLKRVFDGLQNPLEDI
                     AEKSGYFLPRGVYLDPLPRDTKWKWHPIAKVGDKVERSHFLGWVPEENIQHKIMVPFN
                     FPGKGIVVEIASGGEFTVDQEIAKIEDEKGKVHSVNMIQVWPAKFALRFGERLLPKEQ
                     LVTAMRVIDGPFPVAKGGTFCTPGPFGAGKTVLQHQVSKYAKTQIVIIVACGERAGEV
                     VEILREFPHLDDPQTGKKLMDRTCIICNTSSMPVAARESSVYMGVTIAEYYRQMGIDC
                     LLLADSTSRWAQALREMSGRLEEIPGEEAFPAYLASRISELYERAGVVKLYNGEEGSL
                     TIGGSVSPAGGNFEEPVTLATLSVVGCFLGLSRQRSDARRYPAIDPLISWSKYTDRFA
                     EHGDDITKERVKTIAIAQDIVLKSDEIGKKMSVVGEEGISIDDIIVYLKGEFYDSVYL
                     QQNAFDPVDTATDMDRQNEQIDLVSKVLKRNFKFDDKEAARNFFLDLTDKVIQLNYTP
                     KNEFNYKDKKEQILNMIEGE"
     gene            complement(4198..5040)
                     /locus_tag="PL056_10105"
     CDS             complement(4198..5040)
                     /locus_tag="PL056_10105"
                     /inference="COORDINATES: protein motif:HMM:NF022415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2764 family protein"
                     /protein_id="HPM47407.1"
                     /translation="MMKFNYYTIGALPKLTWDSELPCTISEFIEEYRVQLEPLMDGVS
                     DILLLNDVKNLELILKARIEKDEKFRGNKDDGKIDYYKARVVEPEELESFLEEPFINR
                     PDENYPEFMIEYFLKYKTDEERIHHIEGLYIDYFRYMQTRKNGFLRYYGRIATTIRTV
                     LSAMRIMRRGLDLDKNLKGDPFVVQTILENRNNADLGLKNHFPKVAEVIALFDRDRDP
                     IEVEKDLDRIRFELMEEVGRETPFADHIIYSYIIGFQVRNRWNSLNDEKGLQILENII
                     EGNY"
     gene            complement(5059..5691)
                     /locus_tag="PL056_10110"
     CDS             complement(5059..5691)
                     /locus_tag="PL056_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47408.1"
                     /translation="MSDAQSVKISSLDSKIKDISDYLKSSVLDPAEKEKESIIASAKE
                     ESAKIIADARKQAEDMLAKAKKESDALKHNTDSALKIAAKQSVDKLKMALEKEVLSFT
                     VDKPVKDSLKSEHVIKELVAGVVKHYSDKGAFAIVLSDELKKTLSSYVQEQVSLKGAA
                     GIKLSDETIPSGFSIVSGNGVLKYDFSEESVVELLTEFIRPELRKALFSK"
BASE COUNT         1487 a         1444 c         1074 g         1850 t
ORIGIN      
        1 ttgtcgtgaa atatctcaat ttcagcaata gcttcttcaa tttttacctc atgggcaact
       61 ttctgggcta catcccaaag ctgaataaaa acattccctt tctgctctat ctctctgata
      121 tctttgatca gaaaatcgcc ccatggagca atgaaattca gtttgttctg gagctgaacg
      181 gtttcttccc tgcattttct gtgctctctg tcaagaacgg gtattctgtc aacaagagtc
      241 gccggatcac cttcgcagtc atttcttgtc aggcctgttc tttcagcagc ggcagcatat
      301 ctctcaactg tcttgatcgc ccagattatc aggtcggccc ttactgttga aacttctcta
      361 ccgtcaacgg gaaaaagcga tcctttatac gactccactt caacaacgcc cgatctttgc
      421 agtgcagaaa gcactttttc cttattttca gcaggcccca caaggagaag tttctgcatt
      481 cctgttatca ctataccgcc tccgcaaact gcatttcatc ttttttcttt aatttccctt
      541 ttgctatttt ggcattgcat attgccgcaa cttcctgatc tcctagaaat atttttattt
      601 ttcttatgtt ctctctacac tccggtatca tcctcttttc aaacagattg accttaatgc
      661 tcgtctgtct gagctcttcg gaaagaagtt tcatgcgctc tcttattatt ttgagcttct
      721 ccctttcgac aactatcgct ctgagctctt cataagccct gtcaacccat accggcttga
      781 tgaaaaaaga gtatgagaac ggtttgaact taagatcttt gaaaaccctg acatcaactc
      841 cggcaatatt ttcagtgtct gtaacgatct cttcgatctc aaccatctga tacacattta
      901 ttgtaagctt ctgacttaaa accaccgccc agttggaaac ataatctttt cttcttttaa
      961 tctcggcaag tattgaagat tcaatgtttt ttgtattctc cacttccgcc tgcaactgct
     1021 gtttcttgag ttggagggta ggaagatatt ttgtcagctg ggcaagaaga tttttctgta
     1081 ttttaagctc agttttattc agtttaatgg ctgacattac ttatcccatt ttccgtactt
     1141 ttcaatccac ttgttcttta aacccgtttc gttcctgtca aaacagccgg caagtatctg
     1201 ccagccgagg ttcaattgct cttcaatatc gagatttaca cgaaggtcca tcattttctc
     1261 ttcaaaaaga tatgcataac ttaaaagttt cttgtcccat ccggacagtc tgaatcccat
     1321 cgattctctg tcgcgcgcct tttttgcatc tgcataaagc cggatcattg cgttggcaag
     1381 gtctccgtga tcctctcttg taactttgcc cattacctgc tgtttcaaac ggcttagtga
     1441 accgaaagga tcgatccttc cgccgtgaag atagaactga ccttccgtga tgtagcccgt
     1501 attatccgga acaggatggg ttacatcgtt acccggcatt gtagttacgg caatgatcgt
     1561 aattgagccg gagctttcta tgtcgaccgc cttttcatat cttgcggcaa gatcgctgta
     1621 aagtgagccg ggataacccc tgtttgaagg gaccatgtcc attgatattg aaatttcctt
     1681 taatgaatcg ctgaatgccg tcatatcggt aagaagaacc agaacatctt tattctgaat
     1741 ggcaaatttt tccgccgcgg caagagccat atcaggaaca aggatgcact caacgacagg
     1801 gtctgatgcg aggtggatga acattgatgt cttttccatc gcgcctgttt ttgtaaaata
     1861 atctttaaaa aactgatagt catcaaactt cagacccatt ccgccgagaa tgattatgtc
     1921 ggcatcagtc tggcttgcaa tacgggcaag aagctcgtta taaggttccc ccgggattga
     1981 aaaaatgggt attttctggc tgatgacaag tgcattgaac atatcgatca tcggaatgtt
     2041 cgttttgaca aatcttttcg ggacaattct ttttgaaggg ttgaaagaag gtcccccgat
     2101 atcaactttc tcttccatga gttccggacc gttatctatc ggctctccgc tgccgttgaa
     2161 aactctgcct agcatgcttt caccgcatcc gacctgcatg ggcttgccta aaaatcttac
     2221 ctggtcccct gtgctgacac cttttgtgcc aagatagacc tgaagattaa ctctgtcgtg
     2281 atcaaagctt attacctcac cgagagtctc ccttccgtcg ctcattttta ttctggcaag
     2341 ttcacccagc atgacacctt cggccttgac ctgagcgata ctccctttga tcctttctat
     2401 ccggttgtag gtctttacca tattattcac cctctatcat attgagtatc tgttcctttt
     2461 tgtctttata attgaattca tttttcggcg tgtagttaag ctggatgact ttatcggtga
     2521 gatcaaggaa aaaatttctt gccgcttcct tgtcgtcaaa tttaaagttt cttttaagca
     2581 ccttcgagac gagatctatc tgttcgttct gcctatccat atcggtcgca gtatcaaccg
     2641 gatcaaaagc gttctgctga agatagaccg aatcatagaa ttcacctttg aggtaaacta
     2701 taatatcatc aatgctgatt ccttcctctc cgacaacgga cattttcttt ccgatctcat
     2761 cacttttgag aactatgtcc tgcgcaatag ctatcgtctt caccctttcc tttgttatat
     2821 catcaccgtg ctcggcaaaa cggtcagtgt actttgacca gcttatgaga ggatcgatcg
     2881 ccggatatct tctcgcatca cttctctgac ggctcaaacc gagaaaacat cctacaacag
     2941 aaagagttgc aagcgtaacg ggttcttcaa agtttccgcc tgcgggactt acactgccgc
     3001 cgatggtgag tgatccttct tcgccgttat aaagttttac aacaccagct ctttcataaa
     3061 gttccgatat tcttgaagca agatatgcag ggaacgcctc ttcacccgga atttcttcaa
     3121 gacggcctga catttctctg agtgcctgtg cccatcttga agttgagtcg gcaagaagaa
     3181 ggcagtcaat acccatctgt ctgtaatatt ccgcgatggt gacacccata tagaccgaac
     3241 tttcccttgc cgcaacaggc attgacgatg tattgcagat tatgcatgtt ctgtccataa
     3301 gcttctttcc agtctgagga tcatcaaggt gaggaaattc tctaagtatt tcaacaactt
     3361 cacctgccct ttcaccgcat gcgactatta tgactatctg ggtcttggca tattttgaga
     3421 cctgatgctg aagaaccgtt tttcctgctc cgaaaggacc gggagtacag aatgtccccc
     3481 cttttgccac aggaaaaggt ccgtcaatga ccctcattgc agtaacgagc tgttctttgg
     3541 gaaggagccg ttcaccgaat ctcaaagcaa actttgcggg ccagacctgt atcatattga
     3601 cagaatgaac cttacctttt tcatcttcta tctttgctat ctcctgatca acggtaaact
     3661 ctccgcctga agcgatctca acaacaatcc ccttgccggg aaaattgaac ggaaccatta
     3721 ttttgtgctg tatgttctct tcgggaaccc agccgagaaa atgacttctt tcaaccttgt
     3781 cgccgacttt ggcaatagga tgccatttcc attttgtgtc tctcggcaat ggatcgagat
     3841 aaactcctct gggaaggaaa tatcccgatt tttcagcaat gtcctcaagc ggattctgca
     3901 aaccgtcaaa aaccctcttc agaagtccag gtccgagttc aaccgaaaga agatcatgct
     3961 caaagacgac ttcatcgttc agccggaccc cttttgtcgg ctcgaaaacc tgcacttttg
     4021 cctgattacc gttgatctcg ataacttcac ctttaagcga ctgtccgccc gttttaacaa
     4081 aaacgacttc attctgtctg acatttccat caaactctac agtcatcaga ttgccttgtg
     4141 cggcaacgat ctttcctttt gtcttttcaa cttgcaaaac cattatgaac actcctgtca
     4201 gtaatttcct tctattatat tttcaaggat ctgaagtcct ttttcatcat ttagcgaatt
     4261 ccatctgttg cgaacctgga aaccgattat gtaggaataa attatgtgat ctgcaaaagg
     4321 ggtctccctg cccacttcct ccatgagctc aaatcttatc ctgtcaagat ccttctcaac
     4381 ctctatcggg tctctatccc gatcaaaaag agcaatgact tcagctactt tcggaaaatg
     4441 atttttgagc cccagatccg cattgttcct gttttcaagt atcgtctgga caacgaaggg
     4501 gtccccttta agattcttgt caagatcaag gcctcttctc attattctca ttgcggaaag
     4561 aacggttctt atcgttgtcg ctatacggcc gtagtatctt aaaaatccgt tcttccttgt
     4621 ctgcatatat ctaaagtaat caatgtagag cccttcaatg tggtgaatcc tttcttcatc
     4681 ggtcttgtat ttgagaaagt attctatcat gaattcaggg taattctcat ccggacggtt
     4741 gataaaaggt tcttcaagaa aagattcaag ctcttccggt tcgacaactc tcgccttata
     4801 ataatcaatc ttaccgtcat ccttgttacc tctgaacttt tcatcttttt ctatgcgggc
     4861 tttcagaata agttcaaggt tcttgacatc attgagaaga agaatgtcgc ttactccgtc
     4921 catcaatggc tcaagctgaa cccggtattc ttcaataaac tccgaaatag tgcacggaag
     4981 ttcactgtcc catgtcagct tcggcaatgc tccgatcgtg tagtaattga atttcatcat
     5041 tgagataaaa cctccggatc actttgaaaa aagggctttt cgcagttccg gccttatgaa
     5101 ttcagttaaa agctcaacaa ccgattcttc ggaaaagtca tatttcagca cgccgttccc
     5161 tgacactatc gaaaatccgg aagggatggt ttcgtccgaa agttttatgc cggcagcacc
     5221 ttttaaagaa acctgctcct gcacataaga tgacagtgtt ttcttaagct catcgctcag
     5281 aacgatcgca aaagcacctt tatctgaata atgtttaaca acaccggcaa ccagctcctt
     5341 tatgacatgc tcgctcttta acgaatcttt caccggtttg tcgactgtaa aggaaagaac
     5401 ctctttttca agagccattt tcagtttatc gaccgactgt tttgccgcga tctttagagc
     5461 tgaatctgta ttatgtttca atgcatccga ttcttttttc gctttcgcca gcatatcttc
     5521 cgcctgtttt ctggcatctg cgattatttt tgccgattct tcttttgcag aagcgataat
     5581 gctctccttt tccttttcgg caggatcaag aacagatgat ttcaaataat ctgaaatatc
     5641 ctttattttt gagtcaagcg aactgatctt tacggattgg gcatcactca ttcggtctct
     5701 ccatcaccaa ttatattaaa gtaacaacaa accttgctat cttataaacc acacacaaaa
     5761 agtcaacttg attccaaaat tttttatcac aaaaacccga ccctgtcaag acatttaaaa
     5821 accttaccag agagtcaatc gtcgagacat taaga
//
LOCUS       DAOOYB010000182         5822 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_15872_length_5822_cov_7.516733, whole genome shotgun sequence.
ACCESSION   DAOOYB010000182 DAOOYB010000000
VERSION     DAOOYB010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5822
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15872_length_5822_cov_7.516733"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="PL056_10115"
     CDS             <1..624
                     /locus_tag="PL056_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47409.1"
                     /translation="DTGNTGDTGNTGDTGNTGDTGNTGDTGNTGSIIANYDFSQWAGT
                     PELPVAWNAGDYETPVITLAKFDRGSGNFALQISHASHDKNIYAVTTEPFNAVDALPT
                     KIKFELATDQTSKMSININCPNGTAPADQVRTFYIFDSANKKFINNDQNQYNVIDFGD
                     TDFHQTEIVVGSEVTAAMWNSAKQCLLEFKSGKNVDFNIKIDNVIVE"
     gene            complement(643..4806)
                     /locus_tag="PL056_10120"
     CDS             complement(643..4806)
                     /locus_tag="PL056_10120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HPM47410.1"
                     /translation="MNDRLGIDIGTTFITVFSQNRNSVIVRKRHRGKAASILKKIFGE
                     AGTSSSVKFTGKSAKEFSSVLNEAYIPETTAINNYLKKHSPFMSGSGTIIDIGASSLT
                     KYVIKNNAISDISSNSLCAAGTGLFLEEQAERLGIDLEKASSLDIDDPPNIASRCTVF
                     AKSDLIHHQQEGRTKDEMWAGMCKALAVSAANTLFKGAEIKGEIAIIGGVSMNCEVVR
                     WFKKLFPLAFWVIPENSEAITAIGAMEGAVTPSTKIDLDSFIPPVKDELMPPLKLRKS
                     KYPDFIDPSVDVLKNEIRIHSDISKISDVIIGMDIGSTSTKIAMLSTNGTPLLDIYRK
                     TEGDPVNASKKIFKALFSLINPIETKIVSFGTTGSGRKLVGEIFGADAIVNEITAHGR
                     GAASFFPEVETIFEIGGQDAKYIRMKNGFVNDVNMNYVCAAGTGSFVEEQSRKLDIPV
                     QNAGTVTENIAPPVTSDRCTVFMEQDIRSLLKQGYMKEEVLASVLYSVTKNYLNRVVG
                     NRKISRDKIFFQGATARNKGLVAAFENLLDVEVVVSPFCHVMGCIGAALIAYDEVTQG
                     AINHAPTPLTTHDSPLTISPSRFIGPDSVDMEVTSRTEICKLCNNLCRINFIKRNGAD
                     EFSWGYMCGREPGLDKRKEIDEFRMIRERNREFYKSSNIDNAIGRIGIPMALSMHTYY
                     PLWLKMFEKLNIKIEISSTVTSARIKAAGSKYSSSDFCFPMKASIGHAAELIERKIPI
                     FFPSMIAAEQSVKTAISFFCPYVESAPSVIISSLEKNGIDASSYILDPVIDLRLSVEK
                     NSEYIFNKLKKLFPVTKKEVVKAFGAAYSHFIIKEHEQTEKGENYLAEMTAAKKPVFL
                     LVGRPYNLYDKGINLGIPETVASMGYAVVPMDMLELDTSGFDKTNYWNLFWNYGQKII
                     AALKMAASNEMLFPIYLTNFSCGPDSFILSYAEEEMKGRPMLILELDEHDSDGGYRTR
                     IEAYIDVVKGYLKNKEKSAEKPMPDEFLADRPLDKGKVWTPPMHRVGTPLFAAAFRGY
                     GYDSEPLPPETRNEFNLGKRYTRGAECLPMTLTLGAILNQAQLDPSRKHILFMPTSEG
                     PCRFGQYNLLERIAFHNAGISNIDLMSPSSINSYQGLEEPLRRYLMHAMMSSDIMMKL
                     LTKTRPYEKTKGDTDTLFAESVKLLEKTLEKKDDPKPVIKEITKKFKDIPKFEGRKPL
                     VGIVGEIYARCNDYANGNIIEVIEKNGGEAWLSPMHEWILYTAWLQNYMAKQKSFSLF
                     EAGESLIKNIYLFRTESAYYKSSHEVLHDRHEPPVEDVVTEGMKYLPPEFSGEAILTV
                     GRTVMFAKEGASMVVNIAPFGCMHGTITDSIFQEIKTKHKMAILSQFYDGDIDINDKV
                     ADMLAMMKK"
     gene            4965..5279
                     /locus_tag="PL056_10125"
     CDS             4965..5279
                     /locus_tag="PL056_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47411.1"
                     /translation="MLDILRVFAFVSVFVLSGFIMIVGESVADSSNQVEAYYEFEEDL
                     VVSVPVVEMPEILFAEAEIAVPMSYVEMADLEINVIDERLAQNCSVEWNGSIQNEAAC
                     CV"
     gene            complement(5298..5759)
                     /gene="bcp"
                     /locus_tag="PL056_10130"
     CDS             complement(5298..5759)
                     /gene="bcp"
                     /locus_tag="PL056_10130"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HPM47412.1"
                     /translation="MLKKGDKAPEFRLQDSNGTEIKLSDFLGNNVVLYFYPKDNTSGC
                     TTEALDFSALSDKFRKKNAVIIGISPDSVTSHKKFEEKHGLKVLLLSDTEKDVLQKYG
                     VWQLKKMCGRESMGVVRTTIVIDKNGRIADIFEKVKVAGHADKVYGSVCEL"
BASE COUNT         1549 a         1244 c         1201 g         1828 t
ORIGIN      
        1 gacacaggca ataccggcga cacaggcaat accggcgaca caggcaatac cggcgacaca
       61 ggaaacaccg gtgacacagg caacaccggc tcgatcattg caaactatga tttcagccag
      121 tgggcaggaa ctcctgaact ccccgttgcg tggaatgccg gggattatga aacacctgta
      181 ataacacttg caaaatttga caggggttca ggcaatttcg cccttcagat ttctcacgca
      241 tcccacgaca aaaatattta cgctgttacg acagagccat tcaatgctgt tgatgctctt
      301 ccaacaaaaa ttaaatttga acttgcaaca gatcagacat caaaaatgtc tatcaacatt
      361 aactgtccaa atggcactgc tccggctgat caggtgagaa cattctatat ctttgattca
      421 gcaaataaaa agttcataaa caatgatcag aaccagtata atgtcattga ttttggtgac
      481 accgattttc atcagacaga aattgttgtc ggttctgagg taactgctgc aatgtggaat
      541 tcagcaaaac aatgccttct tgagttcaaa tcagggaaaa atgttgattt caacatcaag
      601 atcgacaatg tgatcgtcga ataatcggaa tttataaaga tcttattttt tcatcattgc
      661 aagcatatcg gcgactttat cgtttatgtc gatatctccg tcgtagaact gtgagagaat
      721 agccattttg tgttttgtct tgatctcctg gaatattgag tcagtgatgg tgccgtgcat
      781 acagccgaaa ggtgcgatat tgacaaccat tgaggcacct tcttttgcaa acatgacggt
      841 tctgccgacg gtgagtatcg cttcaccgga aaattcgggg ggaagatatt tcattccttc
      901 tgtgacaaca tcttcaacgg gcggttcgtg gcggtcatgg agaacttcgt gtgatgattt
      961 gtagtatgca ctttctgttc tgaaaagata tatgttcttt ataagtgatt ctcctgcttc
     1021 aaaaagagag aacgacttct gtttagccat atagttctga agccatgcag tgtaaaggat
     1081 ccattcgtgc atcggtgaaa gccatgcctc tccgccattc ttttcaatca cttcaatgat
     1141 attaccattt gcataatcat tgcaccttgc gtagatctcg ccgacgattc cgacaagcgg
     1201 tttccggcct tcaaatttgg ggatgtcttt aaatttctta gttatttcct tgatcacagg
     1261 cttaggatca tcttttttct ctaatgtttt ttcaagaagc tttaccgatt cagcaaaaag
     1321 ggtgtcggtg tctccttttg ttttttcata aggtcttgtc tttgttagga gcttcatcat
     1381 tatgtcactt gacatcattg cgtgcatcaa atatcttctc agcggttctt caagaccctg
     1441 atacgaattg atggatgaag gactcataag atcgatgtta gatatccctg cattatgaaa
     1501 agcaattctt tcgagcagat tgtactgccc gaacctgcat ggaccttccg aagtgggcat
     1561 gaaaagaatg tgttttcttg acggatcaag ctgtgcctga ttgagaatcg cccccagagt
     1621 caaggtcatc ggaagacatt cagcccctct ggtgtatcgt tttccaaggt tgaattcatt
     1681 tctcgtttcc ggtggaagcg gttcagagtc atatccgtag cccctgaatg ccgcagcaaa
     1741 aaggggagtt ccgaccctgt gcatcggagg agtccatact ttgcctttgt caagaggtct
     1801 gtcggcgaga aattcgtcag gcatcggttt ttcagcactt ttttccttat ttttgagata
     1861 tcctttaaca acatcgatat atgcttctat ccttgttctg tatccgccat ctgaatcgtg
     1921 ttcgtcaagc tcaaggataa gcatcggacg acctttcatc tcctcttcgg cataagagag
     1981 gataaaactg tcaggaccgc aactgaaatt tgtcagatat atcgggaaca gcatctcgtt
     2041 cgacgctgcc attttcaatg ccgctattat tttctgaccg taattccaga ataaattcca
     2101 gtagttagtt ttgtcaaagc cagatgtgtc aagctcaagc atatccattg gaacgacagc
     2161 atatcccatt gatgccacag tttcaggaat tccgagattt attcccttat catataggtt
     2221 gtaaggtctt cctacaagaa ggaacaccgg ttttttcgca gcagtcattt ctgcaagata
     2281 gttttccccc ttttctgtct gctcatgttc tttaattata aagtgtgaat aagcggctcc
     2341 gaatgcttta acaacttctt tttttgtaac aggaaagagc tttttcagtt tgttgaagat
     2401 atattcgctg tttttttcaa cacttaagcg cagatctatt acggggtcca gtatgtatga
     2461 agaagcatcg atgccgtttt tttcaagaga gctgatgatc acagaaggag cgctttcaac
     2521 ataggggcag aagaaactta tagcagtttt cacactttgt tcagccgcga tcatggatgg
     2581 aaagaaaatg ggaatttttc tttctatcaa ttcagccgca tgtccgatgg atgctttcat
     2641 cgggaaacag aaatcagagc ttgaatattt agatccggcg gcttttattc ttgcggaagt
     2701 gactgttgac gaaatttcaa tttttatgtt gagtttctca aacattttca gccacaatgg
     2761 gtagtaggtg tgcatagaaa gagccatcgg aataccgatc ctgccgatcg cattatcaat
     2821 attcgagctt ttataaaatt ctctgttccg ttccctgatc atacggaatt catcgatctc
     2881 ttttctcttg tcaagtccgg gttccctgcc gcacatgtag ccccaggaaa actcatctgc
     2941 accgtttctt tttataaaat ttattctgca cagattgttg cagagtttac aaatttctgt
     3001 tcgcgaagtc acttccatat ccactgaatc gggacctata aatcgtgacg gggaaatcgt
     3061 gagtggtgag tcgtgagtgg tgagcggtgt aggggcgtga tttatcgcgc cctgtgtcac
     3121 ctcgtcgtat gcgatgagtg cggctccgat acagcccatg acatggcaga aaggggagac
     3181 gaccacttca acatcgagaa gattttcaaa tgccgcgaca agtcctttat ttcttgcagt
     3241 tgccccctga aaaaatatct tgtcccggct gatctttctg tttcccacaa cacggttgag
     3301 atagttcttt gtgacggaat acagcactga tgcaagaacc tcttctttca tatatccctg
     3361 ctttaaaaga gaccggatgt cctgctccat gaaaacggta catctgtcgc ttgtaacagg
     3421 cggagctata ttttcggtga cagtgccggc gttctgaaca ggaatatcga gctttcttga
     3481 ctgttcttca acaaaagaac cggttcctgc cgcacagaca tagttcatgt tcacatcatt
     3541 tacgaaaccg tttttcatcc tgatatattt tgcatcctga cccccgatct cgaaaatcgt
     3601 ttcaacttca ggaaaaaatg atgcggcacc tctgccgtgt gctgttatct cattcactat
     3661 tgcatcagct ccgaatatct ctccgacaag ttttctccct gagccggtgg ttccgaatga
     3721 aacaattttt gtttcaatag gatttattaa tgagaacaat gccttaaata tcttttttga
     3781 ggcatttaca gggtctccct ctgtttttct gtatatatca agaaggggag ttccgtttgt
     3841 tgaaagcata gctatttttg tgcttgtcga gccgatgtcc atccctataa taacatcact
     3901 tatctttgaa atatcggaat gtattctgat ctcgttcttt aaaacatcga cagaagggtc
     3961 tataaaatca ggatacttgc tttttctgag tttaagcggc ggcattaatt catctttcac
     4021 aggggggatg aaactgtcaa ggtcgatttt tgtggagggt gtgaccgctc cttccattgc
     4081 ccctattgca gtgattgctt cactgttttc cggaatgacc cagaatgcaa gtgggaagag
     4141 ctttttaaac catcttacaa cctcacagtt catgcttact ccgccgataa tggcgatctc
     4201 tccttttatt tctgcgcctt tgaaaagggt gttcgccgca gaaacggcaa gcgctttgca
     4261 cattcccgcc cacatttcat cttttgtcct tccttcctgc tgatggtgaa taagatcact
     4321 ttttgcaaaa acagtacatc tgcttgcaat attcggcgga tcgtcaatat caagtgaaga
     4381 ggccttttca agatctattc cgagccgttc tgcctgttct tcaaggaaaa gaccagtgcc
     4441 tgccgcacag agcgaatttg aggaaatatc acttatcgcg ttgtttttaa tgacatattt
     4501 agtgagtgat gatgcaccga tgtcaattat tgttcctgag ccgctcataa aaggtgagtg
     4561 ttttttcaga tagttattta tggctgtagt ttcagggata tatgcttcgt taagcacaga
     4621 agagaactct tttgccgatt ttccggtaaa tttaactgat gaagaggtgc cggcttcacc
     4681 aaaaatcttt tttagaattg atgccgcttt tccccggtgt cttttcctga cgataacact
     4741 gtttctgttt tgagagaaaa cagtaataaa agtagtacca atatcaattc cgagacgatc
     4801 attcataatt ttccacccca tatagagaac aattacggaa acaggaggtt ttattagata
     4861 gagagaaaga tttcaagaaa aaactttcaa ttaggtcttg acagtccgaa ttactgctta
     4921 tattagaaac tgtactttaa gttcatttaa agatggaggt tgtcatgtta gatattttaa
     4981 gagttttcgc ttttgtttct gtgttcgttc tttccggatt tataatgata gtgggggaga
     5041 gtgttgcaga ttcaagcaat caggtcgaag cttactatga atttgaagag gatcttgttg
     5101 tttcggtccc tgtagttgaa atgcctgaaa tattattcgc agaagcggaa attgccgttc
     5161 caatgagcta tgtggaaatg gccgatcttg aaatcaatgt cattgatgaa aggctcgctc
     5221 agaactgctc agtcgaatgg aacggttcaa tacaaaatga ggccgcctgc tgcgtttagc
     5281 ggcggcttca ttcttcttta aagctcacaa acagatccat agactttatc tgcgtgtcct
     5341 gccactttga ctttttcaaa aatgtcggcg atacgaccgt ttttatcaat gacgatcgtt
     5401 gttctcacaa ctcccatcga ttctcttccg cacattttct ttaactgcca gacaccgtat
     5461 ttctgaagaa catctttttc cgtgtcgctt aagagaagaa ctttaagtcc atgtttttct
     5521 tcaaactttt tatgtgaagt cacagagtcg gggcttatcc cgattatcac ggcatttttc
     5581 tttctgaact tgtctgataa agctgaaaaa tccaatgctt ccgttgtgca gccggaagtg
     5641 ttatctttcg gataaaaata aagaaccaca ttgtttccca gaaaatcaga aagctttatc
     5701 tctgttccgt ttgaatcctg caacctgaac tccggtgcct tgtctccttt tttcaacatc
     5761 tttttctcct tcaaaaatca gtaaaacaac aaagtatagc agaaatccgt cagattaaaa
     5821 ta
//
LOCUS       DAOOYB010000183         5796 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_15969_length_5796_cov_11.863090, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000183 DAOOYB010000000
VERSION     DAOOYB010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5796
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15969_length_5796_cov_11.863090"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1325)
                     /locus_tag="PL056_10135"
     CDS             complement(<1..1325)
                     /locus_tag="PL056_10135"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HPM47413.1"
                     /translation="MIKILNIITIAALFVISCGKIGESHLWVNFYDPDRDNEKIAEIC
                     GKKECGNVDHEGFELICGKCADTEYCSVDQKCTNGCGENSCGKKDTNTYFGIAPIDCG
                     KCAEDEICGFDNTCSKKDDICYGKECGKVYFLDSDNVQNQVECGECTDDLYCGTTLKC
                     LSKADECAEKCGTITVSAFEGDIDIECGGCEGEFAYCNTGNICAEACQGKECGTHKVT
                     LDSLSEKTFTCGDCGDGNNYCDTANKCKVACTDKECGKDELETFSGFETFECGSCTIP
                     EYCDSTKNCKAGTQAGDAVYDENIVIDTKTDLMWQRSNSEIMTLESAEAYCTTAEIDG
                     FTDWTLPDISKLKTIVSGCAAIQTCEITDTCTDSTCTNADCDGCTNNSGPGPQGLYLA
                     PDIWTYTGDANGRFWSSSSTPDKADSYWFIRFSNGSVSYNFDGTEYFVR"
     gene            complement(1335..2372)
                     /locus_tag="PL056_10140"
     CDS             complement(1335..2372)
                     /locus_tag="PL056_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47414.1"
                     /translation="MKKKLIVLIVILFSLVLHAEKPKIAVMSLEDQSGKLTEKVKNAA
                     STLLRMQLSSSGEFIVIDETRQEKVLGDMIKEMKKESYKMCYDEKCQIPLGQALSADT
                     ILRSTVTELGGLFILGVELIDLAKEAVTKAANAEFDGTESGIGEAVKKVVYDLTVKSA
                     QESKKLKDEEEKRKAQIKLEEEKRKKEEHDLKMKKQREAEIEKRKKEDARMAAYKKEL
                     SEAGFKRKSLALTAFLSGAALTGTGIGLLVYSDKLDVMEHDAYTNYLGSITEDEAVKH
                     RKDVEKYRDQEKVSKIVGGILTGAGAGLILTSIITWSVDSSAEKAVKKKYNISFSLDP
                     VSKMAFLSVNF"
     gene            2490..4274
                     /locus_tag="PL056_10145"
     CDS             2490..4274
                     /locus_tag="PL056_10145"
                     /inference="COORDINATES: protein motif:HMM:NF023459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HPM47415.1"
                     /translation="MFTKIRLISIVFAVFFLAVSCGDDLEKNDGDYGDSVSDNETGDT
                     GDSGNTGNSGDTGDTGDSETQDDSGNSGDTGDTGDTGDTGDTGNTGNTGDTGNDGDSM
                     TTDEEQSDLELTDDFVEELSLPDEDVEIPDEEEPQIGDLIENPFIETVEDPVSTFSAP
                     HSTATYTLVRNYIMQRYLMPDKNDIRIEEMVNYFDYNFPEPVGSEPFSYSMEMGPSPW
                     TTGRDLVMIKIKGKDIDAAHNLDKNLVLLIDVSGSMKYAEKLPLIQKSFKKLVTGLTA
                     NDKVAIVSYSTTAKVVLPSTSGDNKTAINNAIDSLIASGQTNVSNGLELAYEEAAKGF
                     IEGGINRVVLATDGDFNTGLDNSEMLQFVATQRQKNNIFMTVLGFGIIDYDDSIMQVI
                     ADNGDGNYYYVDTEKEAYRIFVQKLQSTLFTIAKDLKIQVEFNPEVVERYRLIGFETR
                     LMEDSEFSNDKKDGGEIGAGHYVTAFYEIEKTPQAQTPPVVLKGEVFTQDEALELRIR
                     YKNLNEDFSRYLSKKLINSNNTTVSEDMGFASAVVEYAMLLRRSAYKENSSYDNVIAR
                     ATQYLGLDTWGLREEFIDIVKKTKELDQ"
     gene            4534..5592
                     /locus_tag="PL056_10150"
     CDS             4534..5592
                     /locus_tag="PL056_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015943469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HPM47416.1"
                     /translation="MSRDKQEKARNKAEKQLIIRNSTAEFLIFTIQAGEDGIEVRVED
                     ENIWLTQKLIAKLFDVRIPTINEHLRNIFSTNELQENSVVRNFLITADDGKQYNTKHY
                     NLEAIIALGYRINSERATNFRRWATQVLKDFALRGYVLDNVRLKNGAYLSKQYFKDLI
                     LEIREIRESERNFYQQITDIYSTAIDYDLHSPTTKTFFATVQNKLHYATHGHTAAELI
                     VNRANHKKECMGLTTWKKAPDGKIIKSDVVVAKNYLSKEEIKILNRIVSMYLDYAENQ
                     AEKGIPMTMNDWAEKLNAFLKFNDAEVLQNAGEITAEIAKSFAESEFEKYRPVQDRLF
                     ESDFDREIKKLIGKTTEK"
     gene            complement(5602..>5796)
                     /locus_tag="PL056_10155"
     CDS             complement(5602..>5796)
                     /locus_tag="PL056_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47417.1"
                     /translation="MKNGNYEYKLLYSGQLSPIARLNDEDGVVEEYVYGSVAKGYGPQ
                     VGSRVNVPEYMIKDGRKRFL"
BASE COUNT         1705 a         1179 c         1178 g         1734 t
ORIGIN      
        1 catctgacga aatattcagt tccgtcaaaa ttatatgaaa ctgaaccgtt gctgaaacgg
       61 ataaaccagt aggaatcagc tttgtcaggg gttgatgaag atgaccagaa tcttccgttg
      121 gcatcgcctg tgtatgtcca gatgtccgga gcaagataaa gtccctgcgg tccgggaccg
      181 gaattgttcg tacatccgtc acaatcagcg tttgtgcatg ttgaatcagt gcaggtatca
      241 gtaatttcac atgtttgtat tgccgcacag ccactcacaa ttgtttttag ttttgaaata
      301 tcaggcagag tccagtctgt aaatccgtca atttcagcag tagtgcagta agcttctgca
      361 gattcaagcg tcatgatctc tgagttggat ctctgccaca tcagatctgt ttttgtatca
      421 atgacaatgt tttcatcata taccgcatct ccggcttgag ttcctgcttt gcaattcttt
      481 gtcgagtcgc agtattccgg aattgtgcag gatccgcact caaaggtttc aaatccactg
      541 aatgtttcga gctcatcttt tccgcattcc ttgtcagtgc aggcgacctt acatttatta
      601 gctgtgtcac agtagttgtt tccgtctccg cagtcgccgc atgtaaaggt cttttctgaa
      661 agtgagtcaa gcgtcacctt atgtgtaccg cattcttttc cctgacacgc ttcggcacat
      721 atatttcctg tgttgcaata tgcaaattct ccttcacacc cgccgcattc aatgtcaatg
      781 tcgccctcaa aagctgaaac ggttatggtg ccgcattttt ccgcacactc atccgccttt
      841 gataagcatt tcagcgtagt tccgcagtac aggtcatcgg tacattctcc gcactcaact
      901 tggttctgaa cattatcaga atcaagaaaa tagacttttc cgcattcctt gccataacaa
      961 atatcatcct ttttggagca ggtgttgtca aatccgcata tttcatcttc tgcacatttt
     1021 ccgcaatcta tgggtgcaat accgaagtat gtattcgtat ctttttttcc acacgaattt
     1081 tcaccgcagc cgtttgtaca tttctgatca acggagcagt attctgtgtc tgcacatttt
     1141 ccgcagataa gttcgaaccc ttcatgatcg acatttccgc attctttctt tccgcaaatt
     1201 tccgctattt tttcattgtc tctgtccgga tcataaaaat taacccaaag atgtgattct
     1261 ccgatcttac cgcatgagat cacaaaaagt gcggcaattg ttattatgtt cagtattttt
     1321 atcatgttgc acctctaaaa atttactgaa agaaaagcca tttttgagac cggatcaagc
     1381 gaaaaagaga tattatattt tttctttacc gctttttcag ctgatgaatc aacgctccat
     1441 gttatgatag atgtaagaat aagccccgct cctgctcctg tcagaatgcc gccgactatt
     1501 tttgaaactt tttcctgatc gcggtatttt tcaacatctt ttctgtgttt taccgcttca
     1561 tcttcggtaa tggagccaag ataatttgta taggcatcgt gttccatgac atcgagtttg
     1621 tcagaataaa caagaagccc gatacccgtt cctgtaagtg cggctcctga aagaaatgca
     1681 gtaagtgcaa gagattttct cttaaaccct gcttcactca gttccttttt gtatgcggcc
     1741 attcttgcat cctctttttt ccttttttca atttccgcct cacgctgttt tttcattttg
     1801 aggtcgtgtt cctctttttt tcttttttcc tcttcaagtt tgatctgtgc ttttcttttt
     1861 tcctcttcat ctttaagctt tttagattcc tgagcacttt ttactgtaag atcatagacg
     1921 actttcttga cagcttcacc gataccgctc tcagtaccgt caaattcagc atttgccgcc
     1981 tttgtcacag cttccttggc aagatcgatg agttcaactc cgagaatgaa aagtcctccg
     2041 agttcagtaa cagttgagcg aagtatggtg tcggcactta acgcctgtcc gagagggatc
     2101 tgacactttt catcatagca cattttatat gattcctttt tcatttcctt tatcatatcc
     2161 ccgagcactt tctcctgacg ggtttcatca attacgatga attctccaga tgaactgagc
     2221 tgcattctta gaagagtgga agcggcgttt ttcacttttt cagtgagttt gcctgactga
     2281 tcttcaagag acattacagc gatctttggt ttttcggcat gcagaaccag agaaaaaaga
     2341 atcacaatta aaacaataag tttctttttc atttatcacc tcaaattcac gataacaaaa
     2401 aaagtattat ttttttctaa ataaaaacaa cttctttagt atactatata gaacgagttt
     2461 atttaatttt ttttgaggag gatttggtta tgtttacaaa aatccgttta atttcaattg
     2521 tttttgcagt tttttttctt gcagtttcgt gtggagatga tttggagaaa aatgacggtg
     2581 actatggaga ttctgtaagt gataatgaaa ctggcgacac cggagacagc ggcaacaccg
     2641 gaaattccgg agacaccgga gataccggag attcagaaac gcaggatgac agcggaaatt
     2701 ccggagacac cggagatacc ggcgacactg gcgatacagg cgacacaggc aacacaggca
     2761 acacaggtga tacaggcaat gacggtgaca gtatgaccac agatgaagag cagagcgatc
     2821 tggaactgac tgatgatttt gtggaagagc tctctctgcc cgatgaagat gtagagatcc
     2881 ctgatgaaga ggagccgcag ataggtgatc taattgaaaa tccgttcata gaaacagttg
     2941 aagatcccgt ttccactttt tcagcgccgc atagcacggc aacatatacc cttgtcagaa
     3001 attatatcat gcaaagatat ctgatgcctg ataaaaatga tatcagaatt gaggaaatgg
     3061 ttaattattt tgactataat ttcccggaac ctgtgggaag cgagccattt tcatattcga
     3121 tggaaatggg cccgagtccg tggacgactg gtcgcgatct ggtcatgata aagatcaagg
     3181 gaaaagatat cgatgccgct cacaatcttg ataaaaatct tgttctgctt atcgatgttt
     3241 ccgggtcaat gaaatatgcc gagaaactcc ctcttattca gaaatcgttc aaaaaacttg
     3301 ttaccggtct taccgccaat gacaaggttg ccattgtttc ttattcgaca acggctaaag
     3361 tcgttcttcc ctcaacaagc ggtgataata aaacagctat caataatgcc atagattctc
     3421 tcattgcaag cggacagaca aatgtaagca acggtcttga acttgcctat gaagaagccg
     3481 ccaaagggtt tattgaaggg gggataaaca gggtcgttct tgcaactgac ggtgatttca
     3541 atacgggact ggataactcg gaaatgcttc agttcgttgc aacacaaagg cagaaaaaca
     3601 atattttcat gacagttctc gggtttggaa taatagatta tgatgacagc ataatgcagg
     3661 tcattgcaga caacggagac ggaaattatt attatgttga tactgaaaag gaagcataca
     3721 ggatatttgt ccagaaactt cagtcaactc tctttaccat tgcaaaggat ctcaagatac
     3781 aggttgagtt caaccccgaa gttgtagaaa gataccgtct gatcggcttt gagacccgtc
     3841 tgatggaaga cagtgaattc agcaatgaca agaaggatgg cggtgaaata ggtgcaggtc
     3901 actatgtaac agctttttat gaaattgaaa aaactccgca ggcacagaca cctcctgttg
     3961 tgctgaaagg ggaggtcttt actcaggatg aagcgctgga actccggatc agatacaaga
     4021 atctgaacga ggatttcagc cgttatctga gtaaaaagct gataaattca aataatacaa
     4081 cagtttctga agacatgggg tttgcatctg cagttgttga atatgcaatg cttctgagaa
     4141 ggtcggcata taaggaaaat tcatcttacg acaatgtcat cgcaagagct actcagtatc
     4201 tcggtcttga cacatgggga cttcgcgaag aattcatcga tatcgtgaag aaaactaaag
     4261 agttggatca atagctctct attgaagaaa agtcatctta attcagagat ttaaaattaa
     4321 ttcttcaata aaaaacatat gtagatatgg cttcctatga ttacccaagt ggttctttgc
     4381 tgatccacct gcgggcagta gccttccacc tgccatagct gtgcggcgga caggtcggcg
     4441 gagagcccgt tcacatctct ttttttagat ttttctggat ttaaactctt aaaaaattaa
     4501 catagttcag ttgaattacc agaggagatt aatatgagca gagataaaca agaaaaagca
     4561 cgaaataaag cagaaaaaca gttgatcatt cgcaattcaa ctgctgaatt cctgatattt
     4621 actattcagg caggtgaaga cggtattgag gtaagagtag aagatgaaaa tatttggctt
     4681 actcaaaagc tgatcgcaaa actgtttgat gttagaatac cgacgattaa cgaacacttg
     4741 agaaacattt tttctacgaa tgaattacag gagaattcag ttgttaggaa tttcctaata
     4801 actgccgatg atggcaaaca atataacacg aagcattaca atttagaggc gattattgct
     4861 cttgggtata gaataaattc agaaagggct acaaatttca gacgctgggc tactcaagtt
     4921 ttaaaggatt ttgctcttcg cggatatgtt ttagataatg tgcgactaaa aaacggtgca
     4981 tatctcagca aacagtattt taaagacttg atccttgaaa tcagggaaat tagagaaagc
     5041 gaacggaatt tttatcagca gatcactgac atttattcaa ctgcaataga ttatgacctt
     5101 cattcgccga ctacaaaaac attttttgca acggtgcaga acaaacttca ctatgcaact
     5161 cacggtcata ctgcggcaga acttattgta aatcgtgcaa atcacaaaaa agaatgtatg
     5221 gggctgacta catggaaaaa agcacctgac ggaaagatca tcaaatctga tgttgttgtc
     5281 gccaaaaatt atctgagtaa agaagagatc aaaatattaa accgaattgt ctccatgtat
     5341 cttgattatg ctgaaaatca ggcagaaaaa ggtattccga tgacaatgaa tgactgggcg
     5401 gagaagttga atgctttttt aaaattcaat gatgcggaag ttttacaaaa tgctggtgaa
     5461 attacagctg aaatcgctaa atcttttgct gaaagcgaat ttgaaaaata tcgtcctgtt
     5521 caagaccgac tctttgaatc tgattttgat agagaaataa agaaactgat aggcaagaca
     5581 acagaaaaat aaagatcgct gtcacaggaa tctcttcctg ccgtctttga tcatgtattc
     5641 agggacattg actcttgagc cgacctgtgg gccatagcct ttggcgacgg atccatagac
     5701 atattcctca accacaccgt cttcatcgtt cagccgtgca ataggactta actgaccgct
     5761 gtaaagaagt ttgtattcgt agtttccatt tttcat
//
LOCUS       DAOOYB010000184         5779 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_16034_length_5779_cov_7.705625, whole genome shotgun sequence.
ACCESSION   DAOOYB010000184 DAOOYB010000000
VERSION     DAOOYB010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5779
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16034_length_5779_cov_7.705625"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1272)
                     /locus_tag="PL056_10160"
     CDS             complement(<1..1272)
                     /locus_tag="PL056_10160"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPM47418.1"
                     /translation="MYVKRDIEKAISSHLNSANDQFILLSGARQTGKTSILENIRTDK
                     EKLIINLWDESVNTKLIKSAATFEQLEQFLSSAYGFRPDNSKILIIDEAQAGDHLGKF
                     IMQMHRVWKDQRVIFSGSILSRLFRDNIPMPTGRVVEFVLRPLNFVEFLRFRNKENYL
                     DLIPSDISEPIQKEIHDLFMSEYELFLSIGGLPGIVQAYQNKSDHLILFESLVNNFYR
                     DADRFLNDNGSERSSTVQYGSLLEHCLKTIGRHVAFPTTNSSILSTDSPAYRTILPKL
                     LEALRSWHLVYTLEYETKQNTTKKGYSSKKYLFDTGIMNFLLTRGMPAMLKNGSEISA
                     QLFENIICQELVSYAGSTRAVTSFKTNNKAESELDFVVRTGRNIIPIEVKSGEKITEK
                     SLFQMTEFLKINGLKKGFVVYNGVVQIKEIDD"
     gene            complement(1385..2365)
                     /locus_tag="PL056_10165"
     CDS             complement(1385..2365)
                     /locus_tag="PL056_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47419.1"
                     /translation="MQFFKVFIPIIISLMVAVSCTSGEVKECETIFDCPEGYMCDEYF
                     QCVLIHNKNDESSSVDEEQSFPDENDNTVIPDNGQPDENEHVDNNVTPDENDETVVPD
                     EEEDDDDDKSADEDVVDEEYMDEDLIDDDLSDEDLTESEVLTESDEDTNDEDVVIIPE
                     SVTIGEGTDAQWMPIGCRYFKYSRSAALYLSSEIGVSAPITKVAWFDNTGAPTSRPVI
                     IYLKETSAVSGTSDVWLTQTSGAAEVFNGETTTAYGWNEFTLTTPFNYSGTNNLLVLV
                     EMNEGSFCSSDEPALTYSVVPSMHSSLQTDSDTTGNLSVGDNRPNIKLFF"
     gene            complement(2427..5702)
                     /locus_tag="PL056_10170"
     CDS             complement(2427..5702)
                     /locus_tag="PL056_10170"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPM47420.1"
                     /translation="MKKILFLLIITVTLFSACDKSEKSPCDGVDCSGHGVCDDASGRA
                     VCNCDDGFVPSGVLECIEAADPCAGVECEEWESCVNGDCRPVSGRCNEDEDCTGSATC
                     DKETHTCIGPCSGIDCGGHGECKEGGYGSGSGYESGIYCECEDGYVNFVDDGIPTCVD
                     KDDIPDGEDTDHDNPCDGVKCSGKGNCDVVDGGPECICDKGYVAEGLECVADLCAGVV
                     CEEWEECSEGTCELKSGRCYDSDDCTGEDKCDIATHECGEDKCVSVDCENGGVCTIEN
                     SKAVCNCPNGFKPDGTKCVADTTAPTWIGVQWPYVIDVTIGEAGEVVYGQIYMSNGTT
                     GGSVPQNSGWKAQLGVKKDVTSSEYPVISATWRWVDATFNSSYTGNNHEYMAGFPMDK
                     SGDFAYIYRFSLNNGESWWYCDKGVVGDTPGPNFITSANNYPGIATVKGTMCGTEICK
                     PWERCAVDECELLEGRCNTSDDCGMGYECDGTNTCVASEVELIVQNLVRTSNSLSFEV
                     VYSGANEIDMNESVITLNGVDVTDSISYDESTAIFTVSKTGLTPDKYSYLFRLVDAGG
                     NEIEPLFVPVWVGDGRDFADFGWRDAFVYQLMTDRFLNGDTDNDVGNLAGITVETEQW
                     LGGDFRGIINKIKDNYFTDMGVNALWISSPILNPHTASVGGVNPDDLRSFSSYHAYHP
                     VASGYSYTNDFDYNPLNGYDEGPIDTAFGTVDELHELVAEAHKKGIRVIPDFVANHVH
                     SDSNLYQDNKSWFYPYVKCDGNWDSHRIDCWFTEYMPDFNYAGNPAARKAMIDHAIWL
                     VQEFNLDGFRADALKHMDDIFVKEMKTAVKTKIETTVTNHDSPDEAEIFYMVGESLGG
                     WARYHTRADMVQGQVNEDFYSNAKNYLLWGNGKMSDLASWTIGNDTTFLTEQPTMGGA
                     GGFPGAVMGNFFGNHDQDRALSECGGDYSKLKHAQTYLMTAPINVPMLYQGDDIGMTG
                     RAENGLDGGRRKMMKLSGLMSDEADALAYMKKLGNFRKNYEALRYGKRETCVSSDNGW
                     IYKLTYNGQTVIVGINRGSSGYSGTCTGISGTFKNLDGNNVTVTNGSVTVPAHGSVVL
                     GKK"
BASE COUNT         1554 a         1426 c         1026 g         1773 t
ORIGIN      
        1 atcatcaatt tctttgatct gaactactcc gttatagacg acaaatcctt ttttcaatcc
       61 gtttattttg aggaattctg tcatttggaa gagtgatttt tcagtgatct tttctcctga
      121 ttttacttca atggggatga tgtttcggcc tgttctgaca acaaaatcaa gttcactttc
      181 cgctttgtta tttgtcttaa atgatgtaac tgctcttgtt gagccggcat aacttacaag
      241 ttcctgacag ataatgtttt caaagagttg tgcagatatt tcactcccgt ttttcagcat
      301 tgccggcatt ccgcgggtaa gaagaaaatt cattatccct gtgtcaaaaa gatatttctt
      361 tgagctgtaa cccttttttg ttgtgttctg ttttgtctca tattcaaggg tataaacaag
      421 atgccacgaa cggagcgctt caagaagttt cggcagaatg gtccggtatg ccggtgaatc
      481 agttgaaaga attgagctgt tggttgtcgg aaatgccaca tgtcttccaa ttgttttcag
      541 gcagtgctca agaagtgatc cgtattgaac agtgctgctt ctttcacttc cgttatcatt
      601 taaaaaccgg tctgcatcac ggtaaaaatt attcacaaga gattcaaaaa gaatcagatg
      661 atcactttta ttctgatatg cctgcactat tcccggaagt ccgccgatag agagaaaaag
      721 ttcatattca ctcataaaaa gatcatgaat ttccttttgt attggctcag aaatatcaga
      781 aggaataaga tcaagataat tttctttgtt cctgaatctt aaaaactcaa caaaattaag
      841 cggtcttaaa acaaattcaa cgactctccc tgtcggcatg ggaatattgt ctctaaaaag
      901 ccgtgaaagg atcgaaccgg aaaaaataac tctctggtct ttccacactc tgtgcatctg
      961 cataatgaat tttccaagat gatcacccgc ctgagcttca tcaataatga gaatttttga
     1021 attatcaggt ctaaagccgt aagcagaact taaaaactgt tcaagctgct caaaagttgc
     1081 cgcacttttt ataagttttg tgttaactga ttcatcccac agattaatta tcagtttttc
     1141 tttatcagtt ctgatgttct caagaattga agttttcccc gtctgccttg ctccagacaa
     1201 aagaataaac tgatcatttg ctgaatttaa atgacttgaa atcgcttttt ctatatccct
     1261 ttttacatac atctcagccc cctcaaacat tactcaaaaa ggattatctc cgaatttatg
     1321 taagaaatca acataatttc cgccgaattt atgtaaaatt aacacatgaa ttcggcgata
     1381 ttgatcagaa aaacagtttt atgttcggtc tgttatcgcc gactgagaga tttcctgtcg
     1441 tgtcagaatc agtttgtaat gatgaatgca ttgaaggaac aactgaatat gtcaatgccg
     1501 gttcatcgga tgaacagaaa cttccctcat tcatttcaac aagaacaaga agattgttcg
     1561 ttccgctgta attgaaagga gttgtaagcg tgaattcgtt ccagccgtaa gctgttgttg
     1621 tctcaccgtt aaaaacttca gcagctccac ttgtctgcgt cagccagaca tctgatgtgc
     1681 ctgaaaccgc ggatgtttcc tttaaatata tgattacagg tcttgaagtc ggagcaccgg
     1741 tattgtcaaa ccatgcaact ttggtaattg gagcagaaac accgatctca gaacttaaat
     1801 aaagtgccgc cgacctcgaa tatttgaaat atctgcatcc gatcggcatc cactgagcat
     1861 ctgtcccttc tccaattgtc acagattccg gaattattac gacatcttca tcgtttgtgt
     1921 cttcatccga ctccgttaaa acttccgact ccgttaaatc ttcgtcggat aaatcgtcgt
     1981 caatgagatc ttcatccata tattcttcgt ccacaacatc ttcatcagca gatttatcgt
     2041 catcgtcatc ctcttcttca tcagggacaa cagtttcatc attttcatcg ggtgtcacat
     2101 tattatcaac atgttcattt tcatccggtt gtccgttatc cggaataacc gtattgtcgt
     2161 tttcatcagg gaatgactgt tcttcatcaa cggaagaact ttcatcattc ttgttatgaa
     2221 taaggacgca ctgaaaatat tcatcacaca tgtaaccttc aggacagtca aagatcgttt
     2281 cgcattcctt aacttctcca cttgtacatg aaaccgcaac cattaaagaa atgataatag
     2341 ggataaatac tttaaaaaac tgcatttttg cctccgacca aaaaaatctt ataatattat
     2401 attagtatgt tttgaatatg caagaattat tttttgccga ggaccacgct tccatgtgca
     2461 ggaacagtga ctgaaccgtt agtcacagtc acattgtttc cgtcaaggtt cttaaatgtt
     2521 ccgcttattc ccgtgcatgt tcctgaataa ccggatgaac ctctgttaat accgacaata
     2581 actgtctggc cgttgtaggt aagtttatat atccaaccgt tgtcagatga aacgcatgtc
     2641 tctctttttc cgtaacggag agcttcatag ttctttctga aattaccgag ctttttcata
     2701 tatgcaagag catcggcttc atcagacata agtcctgata atttcatcat ttttctcctt
     2761 ccgccgtcaa gaccattctc agctctccct gtcattccga tatcatcacc ctgataaagc
     2821 atcggaacat ttatcggtgc tgtcataaga tatgtctggg catgttttaa ttttgaataa
     2881 tctccgccgc attcactcaa agccctgtcc tggtcatggt ttccgaagaa gttgcccatt
     2941 acagctcccg ggaatccacc tgctcccccc attgtcggct gttctgtgag gaatgtcgtg
     3001 tcgttcccta tcgtccagct tgccagatct gacatcttgc cgttacccca gagcagataa
     3061 tttttagcat ttgaatagaa atcttcattc acctgccctt gaaccatatc agcccttgtg
     3121 tgatatctcg cccagccgcc gagagattct ccaaccatgt aaaagatttc ggcttcatcg
     3181 ggagaatcgt ggtttgtaac tgttgtttcg atcttcgttt ttactgccgt tttcatctct
     3241 ttaacaaaaa tatcatccat atgtttcaac gcatcagctc tgaacccgtc aagattgaat
     3301 tcctgaacaa gccagatcgc atggtctatc atggctttcc ttgcggcagg attccccgca
     3361 tagttgaaat cgggcatata ttcagtgaac cagcagtcaa ttctatggct gtcccagttc
     3421 ccgtcacatt taacataagg gtagaaccag cttttgttat cctgataaag attgctgtcg
     3481 ctgtggacat ggttggcaac aaaatcaggt atcactctta tccccttttt atgtgcctcc
     3541 gcaacaagtt catgaagttc atcaactgtt ccgaaagctg tgtctatcgg cccttcatca
     3601 tatccgttca ggggattgta atcaaagtca tttgtgtatg aataaccgct tgcaacaggg
     3661 tgatatgcat gataacttga aaaactcctc aaatcatcag gatttactcc gccgacactc
     3721 gcagtatgcg gattgaggat cggtgaactt atccacagag cgttcacacc catatcagta
     3781 aaatagttat cctttatctt gttgattatt cctctgaaat ctccgccgag ccactgttcc
     3841 gtctcaactg ttatccctgc aagattgccg acatcgttat cagtatcacc attcagaaac
     3901 ctgtcagtca tcaactgata tacgaaggcg tccctccagc cgaaatctgc aaaatctctc
     3961 ccgtctccga cccataccgg cacaaaaagc ggctctattt catttccgcc ggcatcaacc
     4021 aatctgaaga gatagctgta tttgtccggt gtaagccctg tctttgaaac tgtaaagatg
     4081 gctgttgatt catcataact tattgaatct gttacatcaa caccgtttaa agttataaca
     4141 ctttcattca tgtcaatttc atttgcaccg ctgtaaacca cctcaaaact cagagaattt
     4201 gaagttctta caagattctg gacgatcagc tcgacttcag atgccacaca tgtgttcgtt
     4261 ccgtcgcact cataacccat accgcagtca tcacttgtgt tgcatcttcc ctcaagaagc
     4321 tcacactcgt caaccgcaca cctttcccac ggcttgcata tttcagttcc gcacatcgtc
     4381 cctttcacag ttgcgatgcc gggataatta tttgcggaag ttatgaaatt cggaccggga
     4441 gtgtccccga ccacaccttt atcgcagtac caccagcttt caccgttatt gagagaaaac
     4501 ctgtaaatat atgcaaaatc tccgctctta tccatcggga accctgccat atattcgtgg
     4561 ttatttccag tatatgaact gttgaatgtc gcatcaaccc aacgccatgt ggctgatatt
     4621 acaggatatt cagagctagt cacatctttt ttcacaccga gttgcgcctt ccatcccgaa
     4681 ttttgcggaa cagaaccgcc cgttgtcccg ttgctcatgt agatctgtcc ataaaccact
     4741 tctcccgctt caccgatagt tacatcgatc acataaggcc actggacacc tatccatgta
     4801 ggagcagttg tgtcggcaac acactttgtt ccatcgggct taaaaccgtt agggcagttg
     4861 cagaccgctt tactgttctc gatcgtgcag acgccaccgt tctcacaatc aacggataca
     4921 cacttatctt caccgcattc atgagttgca atgtcacatt tgtcttcacc agtgcagtca
     4981 tcactgtcat aacatctgcc gctcttaagc tcacatgtcc cttcactgca ctcttcccat
     5041 tcttcacaca caactcccgc acagagatct gcaacacatt caagaccttc tgcaacatag
     5101 cccttgtcgc agatgcattc agggccgccg tcaacaacat cgcagttccc tttgccgctg
     5161 cacttgactc cgtcgcatgg attgtcgtga tccgtatctt caccatctgg aatatcatct
     5221 ttatcaacac atgtaggaat accgtcatcg acaaaattca catatccgtc ctcacactca
     5281 cagtaaattc cgctctcata tcctgaaccg ctgccgtaac caccttcttt gcattctcca
     5341 tgacctccgc agtcaattcc tgagcatggt ccgatgcatg tatgtgtctc tttgtcacaa
     5401 gttgctgagc cggtgcagtc ttcatcttca ttgcaccttc cgctaaccgg tctgcaatct
     5461 ccgttcacac agctttccca ttcttcacat tcaactccgg cacaagggtc cgccgcctcg
     5521 atgcattcaa gcacgccgct tgggacaaat ccgtcatcgc agttgcagac cgcccttccg
     5581 cttgcatcat cacaaacacc atgaccactg caatcaacac cgtcacatgg tgatttctct
     5641 gatttatcac atgctgagaa aagtgtgacc gtaatgatta aaagaaaaag gatctttttc
     5701 atgaggacct ccggagatta aaacaaaaac cgatatatta tcttttttta agacatttta
     5761 gtcaagagta ttgtggctt
//
LOCUS       DAOOYB010000185         5738 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_16189_length_5738_cov_10.909731, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000185 DAOOYB010000000
VERSION     DAOOYB010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5738
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16189_length_5738_cov_10.909731"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="PL056_10175"
     CDS             <1..773
                     /locus_tag="PL056_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017251764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HPM47421.1"
                     /translation="MQNYRMKVSNEIMSKKWVTLMFPTPGFAQASNMSMEAFEEFFFN
                     VTTLDYAKMDKAMDPFKQLLEKTDKVHIKGPGRTDITLSIKGLPAIKCAGEYNIPDGE
                     IFTAPVKDSINGVIEYNTPSIYLGTKFDKVILTFENGKIVKHEGSNPEKLEEIFNADA
                     GARYVGEFAFGVNPYIVEPTVNILFDEKISGSIHLTPGRAYGECNNGNDSSIHWDLVM
                     IQTPEYGGGEIYFDGILVRKDGRFVLPELAGLNPENLK"
     gene            789..1562
                     /locus_tag="PL056_10180"
     CDS             789..1562
                     /locus_tag="PL056_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015115222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPM47422.1"
                     /translation="MFDLHNKIAVVTGASAGIGKATAEALASAGCNLILVARRLEKVE
                     EFAQYLKDKYGVKCFCGKLDVRNRDDVERFFKNLPIDFAKVDILVNNAGLAKGMGPMH
                     TNSVDDWETMIDTNVKGLLYVSRAVVPGMVERDRGHIINIGSIAGRGTYPGGGVYCAT
                     KHAVNAITNGLRKDLTNTSIRVSTVDPAFTETEFSIVRFDGDGEKAKKVYEGFEPLLA
                     EDIADAILYVATRPPHVTISDMVIYPTAQSDFHLVNRKN"
     gene            1694..2884
                     /locus_tag="PL056_10185"
     CDS             1694..2884
                     /locus_tag="PL056_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018212344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM47423.1"
                     /translation="MINRDMYLKKLIGFKDHPLIKVITGIRRCGKSTLLELFREYLLE
                     NGVKNDQIISINFEDMNFSNLDDAQKLHDFVDSRLKKNAKNYVFLDEIQKVADFQKAV
                     DGLYIKKNVDIYITGSNAHLLSGELATLLSGRYVEIEMLPLSFKEYIEFTGKNDLSRK
                     FADYVRYSSFPFAVYLNNDAGKVRDYLSGIYNTVILKDVVAKKKIADMLMLESVVRFM
                     FDNIGSLMSTKKIADTMTSDGRKISTHTVESYISALMDSFIIYRAKRYDVKGKQYLKT
                     NDKYYICDIGLRSFLLGNKGGDAGYVLENVIYLELLRRGYDVYVGKVGDKEVDFVAIK
                     QDTVEYYQVALTVRDENKLKTELASLQAISDHNPKYLLTLDDDPPANHNGIKQINAID
                     FLME"
     gene            complement(2894..3403)
                     /locus_tag="PL056_10190"
     CDS             complement(2894..3403)
                     /locus_tag="PL056_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HPM47424.1"
                     /translation="MEKPKVLVVYYSRTGTTEKIGGLIASAVKADVEKLVDKKDRSGV
                     WGYLVGGKDAGFDNETELGELKYDPGEYDLVIIGTPIWAWNMSPAVRTYITKNKDKIK
                     NVVFFTTSGGTSYEKVVPLMEKHLGKDALFKTGFLEKEVKNDSSEMIENLKKISGFVK
                     NFLEKGVEI"
     gene            3549..4355
                     /gene="zupT"
                     /locus_tag="PL056_10195"
     CDS             3549..4355
                     /gene="zupT"
                     /locus_tag="PL056_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HPM47425.1"
                     /translation="MHTENFWFAFALTVFAGLSTGVGSLLAFLSKKINIRFLAGSLGF
                     SAGVMIYVSFVEILPKSMESLGKVFGSEGELIAVISFFAGIAIIALIDNFVPTIENPH
                     EMKDIDDMKGTISESQKKGLLRMGLFSALAIGIHNFPEGLATFVSALEDPTLGLSIAI
                     AIAIHNIPEGIAVSVPVYYATGSRKKAFLYSFLSGLAEPVGALIGFFFLMWFFDESVF
                     GITFGIVSGIMVYISLDELLPTAEKYGEHHIAIYGLISGMALMAVSLLLL"
     gene            complement(4362..5564)
                     /locus_tag="PL056_10200"
     CDS             complement(4362..5564)
                     /locus_tag="PL056_10200"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HPM47426.1"
                     /translation="MKVLVINCGSSSIKYQLFEMSNESVIAKGLAEKIGLPEGCIDLK
                     ITGKEGKIEVNLPLPNHSVALNKIMELMTENGVIMDKSEIKAIGHRLVHAGEYYNGSV
                     PITDDVISKLEECSDLAPLHNPPNILGVKTCLELFPGTFMSGTFDTAFHQTMPDYAYI
                     YPISYEYYEKYKVRRYGFHGSSHLFVSKRAAEMLGKKIEETKIITCHLGNGCSMAAVL
                     GGKSVDTSMGLTPLEGIMMGTRSGDIDPALIFYLTDKHNGDMKAVNNMLNKNSGLKGV
                     SGVSNDMRDVRSARDNGNKRAELALNIFKYRLKKYIASYAAAMGGVDAIVFTGGIGEN
                     VWEVREDSLKGLEFMGVKINSELNKTIFSKETDISAADSKVKVFIIPTNEELVIARDA
                     MILFENQK"
BASE COUNT         1743 a         1154 c         1157 g         1684 t
ORIGIN      
        1 taatgcagaa ctaccggatg aaggtaagta acgaaataat gagcaaaaaa tgggttacgc
       61 tgatgttccc cacccctggt tttgcacagg cgtcaaatat gtcaatggaa gcattcgaag
      121 agttcttttt caatgtgacg acccttgatt atgcaaaaat ggacaaagcg atggacccct
      181 tcaagcaact gcttgaaaag actgacaaag ttcatattaa aggtccgggt agaacagaca
      241 tcactctttc catcaaaggt cttcctgcga tcaaatgtgc aggggaatac aacattcctg
      301 acggcgagat attcacagct cctgtaaaag attcaataaa cggcgttatc gaatacaaca
      361 ctccgagcat ctatcttgga actaagttcg ataaggtcat actcactttt gaaaacggaa
      421 agatcgttaa acacgaggga agtaaccctg aaaaacttga agagatattc aatgctgatg
      481 caggcgcaag atatgtcgga gagttcgctt tcggagtcaa cccttacatc gtcgagccga
      541 ctgtaaacat cctttttgat gaaaagatct ccggctcaat tcacctcact ccgggaagag
      601 catacggaga atgcaacaac ggtaacgatt catcaattca ctgggacctc gtaatgatac
      661 agacacctga atacggcggt ggtgaaattt acttcgacgg cattcttgtt agaaaggatg
      721 gaaggttcgt tcttcctgaa cttgctggac tcaatcccga aaatctcaaa taaataagag
      781 gcattaccat gtttgatctt cacaataaaa tcgcagtggt tacaggtgcg agtgccggaa
      841 taggcaaagc cacagcagaa gctcttgcaa gtgcgggatg caaccttata cttgttgcaa
      901 gacggctcga aaaggttgaa gaatttgctc aatatctcaa ggataaatac ggtgttaagt
      961 gcttttgcgg gaaactcgat gtaagaaaca gagatgatgt ggaaagattt tttaaaaatc
     1021 tgccgataga ctttgcgaaa gttgacatac ttgtgaacaa tgcaggtctt gcaaaaggaa
     1081 tgggaccgat gcacacgaac tcggttgatg actgggaaac aatgatagac acaaatgtca
     1141 agggactgct ttatgtttca agagcggtcg ttccgggaat ggttgaaaga gacagaggtc
     1201 acataatcaa tatcggatca attgccggaa gaggaacata tccgggaggc ggagtatatt
     1261 gcgcaacaaa acatgcggtc aatgccatca caaacgggct gagaaaagat cttacgaaca
     1321 cctccatccg cgtttcgaca gttgaccccg ctttcacaga aacagaattc agcatagtca
     1381 gatttgacgg agatggcgaa aaagcaaaga aagtttatga aggttttgaa ccgctcttag
     1441 cagaagatat agcagatgca atactttatg tcgccacaag acctccgcat gtcacaatct
     1501 ccgacatggt catctatccc acagcccagt ccgattttca tctggtaaac aggaaaaatt
     1561 aacgcaaaag ttttagaact ccactgctaa aacttcatga aaacgcaaag ttttagaact
     1621 ccattgccaa aacttttgac atttggaagg tttttgaata atatatttct gcgttgttca
     1681 tcaggaggcg cagatgataa acagagatat gtacctgaaa aaactgatag gatttaaaga
     1741 tcatcctttg ataaaggtga taactggaat aagaagatgc ggaaaatcaa cgcttcttga
     1801 gctgttcaga gaatatcttc ttgaaaatgg tgtgaaaaat gaccagataa tatcgatcaa
     1861 ttttgaggat atgaatttct ccaaccttga tgatgctcaa aaactgcacg attttgttga
     1921 ctcccgtttg aaaaaaaatg caaagaacta tgtttttctt gatgaaatcc agaaagttgc
     1981 cgactttcag aaagctgttg acgggcttta cataaaaaag aatgttgata tttacattac
     2041 cggttcaaat gcgcatcttc tttccggaga acttgccacg cttttgagcg gaagatatgt
     2101 cgagatcgaa atgctgccgc tttctttcaa ggaatatatt gaatttacag ggaaaaatga
     2161 tctcagtcgt aaatttgcgg attatgtaag gtacagctca tttccttttg cggtgtatct
     2221 gaataatgat gcgggcaagg ttcgtgatta tctgagcgga atatacaata ctgtcattct
     2281 taaagatgtt gtggcaaaga aaaagatagc agacatgctg atgcttgaaa gtgtcgtgcg
     2341 atttatgttt gacaatatag gaagtctcat gtccacaaaa aagatagcag ataccatgac
     2401 ttcagacgga agaaagatat cgactcatac agttgagagc tatatttcag cgcttatgga
     2461 cagtttcata atttatagag ccaaaagata cgatgtgaaa ggaaagcagt atctcaaaac
     2521 caatgataaa tactatatat gcgacatcgg actccgttca tttctgcttg ggaataaggg
     2581 cggtgatgct ggatatgtcc ttgaaaatgt tatatatctt gagcttctcc gccgcggtta
     2641 tgatgtctat gtcggcaaag tcggagataa agaagttgat tttgtggcga taaaacagga
     2701 cacagtcgaa tattatcagg tagctttgac agtcagagac gaaaacaaac tgaaaactga
     2761 acttgcatca cttcaggcaa tatctgatca caacccgaaa tatcttctga cacttgacga
     2821 cgatccgcca gcaaaccaca acggaatcaa acagataaac gcaatagatt ttctgatgga
     2881 ataatttttt accctaaatc tcaactcctt tttcaaggaa gtttttgaca aaaccggaga
     2941 tctttttgag gttttcgatc atttcgcttg agtcgttttt cacttctttt tcaaggaatc
     3001 ctgttttaaa gagtgcgtct tttccaaggt gtttttccat taaaggaact actttttcat
     3061 aacttgtgcc gccggaagtg gtgaaaaaca ctacattttt tattttgtct ttattttttg
     3121 tgatataagt tctcacagcc ggagacatat tccacgccca gatcggagtg ccgattataa
     3181 caaggtcata ttcacccgga tcatatttca gctcaccaag ttcagtttcg ttatcaaatc
     3241 cggcatcttt gccgcccaca agataacccc agactccgct tctatccttc ttgtcaacaa
     3301 gtttttcaac atcggcttta actgcggaag cgataagccc ccctatcttt tcagttgtcc
     3361 ccgtcctgct gtaataaacc acaagaactt tcggtttttc cactgtctcc tccgctgaaa
     3421 ttaaaagagc aaataaaatt gacaaaaaaa gcatggccac ctccatttaa agttccgtaa
     3481 atagtatcat aattggattt ttttccaaat ttaaaataaa atggcgcaat ttgatcttgg
     3541 aggatctgat gcataccgaa aatttctggt tcgctttcgc attaaccgtt ttcgcaggtc
     3601 tttcaacagg tgtcggaagt ttgctcgcat ttctttcaaa aaagataaac atccgttttc
     3661 ttgccggctc tcttggattt tcggcaggtg tcatgatcta tgtatcattt gttgagatat
     3721 tgcctaaatc catggaatca ctaggcaaag tgtttggaag cgaaggcgaa ctaattgcgg
     3781 ttatttcatt ttttgcagga attgcgatca tagctcttat tgataatttt gttccgacga
     3841 tagaaaaccc gcacgaaatg aaagatattg acgatatgaa agggacaata agcgaatcac
     3901 agaaaaaagg actcttaaga atgggccttt tttcagccct tgcgatcggt attcataact
     3961 ttcctgaagg acttgccacc tttgtttccg cacttgaaga cccgactctc ggtttaagca
     4021 tcgcaatcgc aattgcaata cacaacatcc ctgaaggaat tgcagtttca gttccagtct
     4081 attatgcaac aggcagcaga aaaaaggcat ttctttattc gtttctctcc gggcttgcgg
     4141 aaccggtcgg agcattgatc ggctttttct ttctgatgtg gttctttgat gagagcgttt
     4201 ttggcataac tttcggaatt gtttcaggaa taatggtcta tatctctctt gatgaacttc
     4261 ttcccacagc tgaaaaatat ggtgaacacc acattgcaat ttatggactt atttcaggaa
     4321 tggcattgat ggctgtaagt ctgctgctgc tttagaaaag attatttctg gttttcaaaa
     4381 aggatcatgg cgtctcttgc tataacaagt tcttcatttg tcggaattat aaaaacttta
     4441 acttttgagt ctgccgcact gatgtctgtt tctttgctga aaatggtttt attgagttcg
     4501 gaattgatct taacacccat gaattcaaga ccttttaaag aatcttctct cacttcccat
     4561 acattttcac cgattccacc ggtaaaaaca attgcatcaa ctccgcccat tgccgcagca
     4621 taagatgcga tatatttttt cagccggtac ttaaaaatgt taagagcaag ctcagctctt
     4681 ttattgccgt tatctcttgc actgcgaaca tctctcatgt cgtttgaaac gccactcaca
     4741 cctttcaagc cactgttttt attaagcata ttgttcacag ctttcatatc gccattatgt
     4801 ttatctgtga gatagaaaat aagtgccgga tctatgtcac ctgatcttgt tcccatcatt
     4861 atcccctcaa gcggagtgag ccccatggag gtatcaacgc ttttgccgcc aagaactgcc
     4921 gccattgaac agccgtttcc aagatggcat gtgatgatct ttgtctcttc aattttcttt
     4981 ccaagcattt cagccgccct tttggaaaca aaaagatggc tcgatccgtg gaaaccgtat
     5041 cttctcactt tatatttttc gtaatattca taacttatcg gataaatata tgcatagtca
     5101 ggcattgtct ggtgaaaagc ggtatcaaat gttcctgaca tgaaagttcc gggaaaaagt
     5161 tcaaggcatg ttttcacgcc aagaatattc ggaggattgt gtaatggtgc aagatcactg
     5221 cattcttcaa gttttgaaat gacatcatca gtgatcggaa cactgccgtt gtaatattct
     5281 ccggcatgga caagacggtg accgatcgct ttgatctcgg acttatccat tatgactccg
     5341 ttttcagtca taagttccat tatcttgttc agagccactg aatgattggg aagaggtaga
     5401 ttgacctcga tctttccttc tttccctgta attttaaggt cgatacaacc ttccggaaga
     5461 ccgatctttt cagcaagacc ttttgcaata acgctttcat ttgacatttc aaaaagctga
     5521 tacttgatcg aagagctgcc acagttgata acaagaactt tcatattttc ctccaagggg
     5581 tctgataaaa aatcacaaca gagcgatact attccttcaa aaatcgtttt tcaagaaaaa
     5641 aaacggctca tttgataaat ctttcataaa aaattcattt gtgtgatact gactgataat
     5701 taccggatca ttgaggatgt tttattatga aaaaagat
//
LOCUS       DAOOYB010000186         5649 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_16557_length_5649_cov_10.121023, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000186 DAOOYB010000000
VERSION     DAOOYB010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5649
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16557_length_5649_cov_10.121023"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1845
                     /locus_tag="PL056_10205"
     CDS             <1..1845
                     /locus_tag="PL056_10205"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPM47427.1"
                     /translation="SLKNIAEGVLNSEGNVSIQDIDGMIPENSDFGVDDMEMLFNILD
                     DFNVDITIEPSSDDDSPVVEPVTAIMKKGTTTHERGRKDPLRMYMLTIGAVPLLTKDG
                     EVEIAKRIESGEKEIIKILLDFFITFKEIVSIPDKVMNEQLNIKEIVRELEEEIDDDV
                     SDLEEEDEEETPIDDDDDDIDNADKKEKEKASPDKKNDETTKDTNISDKNLALIEMTN
                     AAVEQLKIANRERTKFLKMAESTTIKEETRKKHRENARKIMEKMIGIILEMRLNKIYL
                     NSLCEKIRQYEEKAKQLQNNISHIEQTMNMTFEQMGQIIAKPDKFPLIDQKNSHIAEK
                     YALINQTKRSIKKLEAETALSIDELNKNSKALRDAEEAVRFAKNQLIQANLRLVVSIA
                     KKYNNRGLDFKDIICEGNIGLIKAVDKFEYYRGYKFSTYATWWIRQSITRAIADQGRT
                     IRIPVHMIEMMNKINKTIKELTNKQGFDPSISDVAKHMNLPEEKIRKVWRSARETISL
                     ETPIGEDEDSQLQDFVEDPKTLSPEDYTARQNMAEIIRDLFNGLTPREEKVIRMRFGI
                     GETETYTLEEIGKEMGVTRERIRQIEAKAVLKLKKMLKTRKTSWEDFI"
     gene            complement(1842..3836)
                     /locus_tag="PL056_10210"
     CDS             complement(1842..3836)
                     /locus_tag="PL056_10210"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HPM47428.1"
                     /translation="MNAITNKDAYKNILDVVKELIKSTDIDDMIIKLNTSVQSFMPGT
                     VVYAVLWDKYEEELQIFPTDQNVDKDVISFFRFMLKNENLRFSGKFENSGHKERLRFF
                     RGYHLFWDMTRLEKGIRGAVLGLHTTNADLKKTTSIAETILPQFWSIFSERYNKMIAG
                     KESEMLWKMKGAQSEAPDGSHLNSIELNKLLENLLELALRKIKLKCGCILLLNEKTGE
                     LEFEPRAVKGKSLSILPEKIATDEKSLASIAIKTNEPYICNDTENDPNYYPLFQGIKS
                     SLVVPIEFQKRSIGVIAIESENKDEFSEEDATLIKALAKEATMFIRRAQLFEETSKRG
                     DAIMILGHSEGWKDVEKRIERASRTDATVILRGESGTGKELLAHAIHFNSPRKDKPFI
                     TLNCAAIPSELLESEMFGHVKGAFTGAYYNKTGEFEKAEGGTIFLDEIGDLPIMLQVK
                     LLRTLQSGEVRPVGSSKVARKVDVRVIAATSRDLESMVSNGEFRMDMYYRLHVVPIKV
                     PPLRDYKDDIPNLIENFIREANANFKTSVKRVNKKALSALMQYDYPGNVRQLRNVINQ
                     AVIMADGDTIKPEDIPAEIFSVNNENSEAGDVIPCLENETGLRSYHAEKEKMMEKFTR
                     EYFSALLRHSGGNMAATAKTAGISRVALYKIFKKFGISDV"
     gene            complement(3902..4528)
                     /locus_tag="PL056_10215"
     CDS             complement(3902..4528)
                     /locus_tag="PL056_10215"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006572459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HPM47429.1"
                     /translation="MNGLIGEIDRNYQIKGFSVIGVDEAGRGPLCGPVVTAAVCLFCD
                     IEGLNDSKKLSEKKRLELLPQIIKNSVWAVYSVSPKIIDELNILHATMFGMAKCIERV
                     KRKVEGKQIVLIDGNRVTGKFDLEEAVVKGDSKSVNIAAASILAKTQRDRIMNRLALI
                     YPEYKLDLHKGYATSEHYEILEKIGPCEIYRKSFKLKADIRHEQMKLF"
     gene            complement(4525..5637)
                     /gene="hemW"
                     /locus_tag="PL056_10220"
     CDS             complement(4525..5637)
                     /gene="hemW"
                     /locus_tag="PL056_10220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HPM47430.1"
                     /translation="MKKSGIYIHIPFCVKRCSYCDFYSEVIKDREIMRIYGDKVISEL
                     IERFHELNATEIVSLYVGGGTPSVMDEEFFSDLLNAVSELGCNIKKMEITVEVNPADI
                     SDKWLKSLRNSGVNRISAGVQSFDDNVLKEMNRRVSVAQMKEALPLINDNFDNISIDL
                     IYGIGKDRNITEELENVFKLVNPVHVSAYQYTPPEKKNAPMLIDEDETMKQEEAIVEF
                     LKNRGIEKYEISNYSKKGKESVHNMLYWNFDSWLGIGAGAKSFIENKREHSFYVEDLE
                     SFINGDGLSRYYPDKNELIMEFLLMGLRVVSGIKLNKFNEIFERSFEQMFERKVVKEL
                     VEEELIVIDKSVIRCYEKGLNFLNHVLLRLFEGIKE"
BASE COUNT         1695 a         1225 c         1016 g         1713 t
ORIGIN      
        1 tctttgaaaa acattgccga aggggtgctt aattctgaag gcaatgtttc cattcaggat
       61 attgacggca tgatcccgga gaattcggac tttggcgtag acgatatgga aatgcttttc
      121 aacatccttg atgatttcaa tgttgacata accatcgagc cctcttctga tgatgattct
      181 ccagttgtag aacccgttac cgccataatg aagaaaggga cgacaactca tgagagggga
      241 aggaaagatc ctttaagaat gtatatgctt acgattggag ctgttcctct tctcacaaag
      301 gacggtgaag ttgaaattgc aaaaaggatc gagtccggtg aaaaggagat aataaaaata
      361 ctccttgatt tcttcattac tttcaaagaa atagtcagca ttcctgacaa agttatgaac
      421 gaacagctta atattaaaga aatagtgcgt gaacttgaag aagagatcga tgatgatgtg
      481 agcgatctgg aagaagagga tgaagaggaa accccgatcg atgacgatga tgatgatata
      541 gataatgctg ataaaaagga aaaggaaaag gcatctcctg ataagaaaaa tgatgaaacg
      601 acaaaagaca caaacatttc agataaaaac cttgccctca tagagatgac aaatgccgca
      661 gttgaacagc tcaagatcgc aaacagggaa agaaccaaat tccttaaaat ggcggagtct
      721 acgaccatta aagaggaaac aagaaagaaa cacagggaaa atgcaagaaa gatcatggaa
      781 aaaatgatcg gtataatcct tgagatgcgt cttaacaaga tctatctcaa ctctctttgc
      841 gaaaagatcc gtcagtatga agaaaaagcg aaacagcttc agaacaacat atcccacatt
      901 gaacagacaa tgaacatgac ttttgaacag atgggtcaga tcattgcaaa acccgacaaa
      961 ttccctctca ttgaccagaa aaattctcac atagcggaaa aatatgcact tatcaaccag
     1021 acaaaaagaa gcatcaagaa acttgaagct gaaacggctc tttccataga cgaactgaat
     1081 aaaaactcca aagcgctcag agatgcggaa gaagctgtga gatttgcaaa gaatcagctt
     1141 attcaggcaa atttgcggct tgttgtaagt atcgcaaaga aatacaacaa ccgcggtctt
     1201 gatttcaaag acatcatctg cgaaggaaac atcggtctca tcaaagccgt tgacaaattt
     1261 gaatattacc gcggatacaa attctcgacc tatgccacct ggtggatccg tcaatctatc
     1321 acaagagcaa ttgctgatca gggcaggacg ataaggatac ctgttcacat gatcgaaatg
     1381 atgaataaga tcaacaagac cattaaagag ctcacaaaca aacaggggtt tgatccgagc
     1441 atatcggatg ttgcaaagca catgaacctt ccggaagaaa agataagaaa ggtttggaga
     1501 agcgcaagag agaccatctc acttgaaact ccgatcggtg aagacgaaga cagccagctt
     1561 caggattttg ttgaagatcc gaagacactt tctcccgagg attataccgc aagacagaac
     1621 atggcggaaa taatcagaga tcttttcaac ggtctcacgc ccagagagga aaaagttata
     1681 cggatgcggt tcggcatcgg agaaactgaa acctatacgc ttgaggaaat aggcaaggaa
     1741 atgggtgtta ccagagaaag gatccgccag atagaagcaa aggcagtgct taaactcaag
     1801 aaaatgctca aaaccagaaa aacttcctgg gaagatttca tttaaacatc agatattccg
     1861 aatttcttaa atattttata aagcgcaact cttgatatgc ctgcagtttt tgcagttgca
     1921 gccatatttc cgccagaatg tctgagaagt gctgaaaaat actctcttgt gaatttttcc
     1981 atcatcttct ctttttctgc atgataactt ctgagtccgg tttcattctc aagacaagga
     2041 ataacatctc cggcttcaga attctcatta tttactgaaa agatctctgc cgggatatct
     2101 tccggcttta tagtgtctcc gtcagccatt atgaccgcct ggtttatcac atttctaagc
     2161 tgccttacat tgcccggata atcgtactgc ataagtgcgg aaagggcttt tttgttaacc
     2221 ctttttacag aagttttaaa atttgcattg gcttccctga taaaattctc gataaggttg
     2281 ggaatatcat ctttataatc ccttaacggt ggaactttta tcggcacaac atgaagccgg
     2341 taatacatat ccatcctgaa ctcaccgtta ctgaccattg attcaagatc acggcttgtg
     2401 gcggcaatga ccctgacatc aacctttctt gcaaccttgc tgcttccgac aggtcttacc
     2461 tctccactct gaagggttct taacagtttt acctgaagca tgatcggaag atcacctatt
     2521 tcatccagaa agatcgttcc cccttccgcc ttctcaaact caccggtttt gttgtaatac
     2581 gctccggtga aagcaccttt gacatggccg aacatttcac tttcaagcaa ttctgaaggt
     2641 atggcggcac aattaagcgt aataaagggt ttgtcttttc tcggactgtt aaaatgtata
     2701 gcatgtgcca gaagctcttt tcccgttccg ctctctccgc gaagaatgac tgtcgcatct
     2761 gtacgggacg ctctctctat ccttttttca acatccttcc agccctcgga atgtccgagt
     2821 atcattatcg catcacctct ttttgatgtc tcttcaaaaa gctgtgcacg ccttatgaac
     2881 attgtggctt cttttgcaag cgcttttata agtgtagcat cttcttcgct gaattcatcc
     2941 ttattctcag attctatcgc aataacaccg atcgatcttt tctgaaactc tatcggaacg
     3001 acaagagatg attttattcc ctgaaaaagc ggataatagt tcggatcatt ctctgtgtcg
     3061 ttgcagatat acggttcgtt tgtctttatt gctattgatg caagactttt ttcatcagta
     3121 gctatttttt caggcaggat ggaaagcgac tttcctttta cagctctggg ctcaaactcc
     3181 agttcgcctg tcttttcatt taacaaaagg atacaaccgc atttaagctt tatttttctc
     3241 aatgcaagtt ccagcagatt ttcaagaagc ttgttaagtt ctatgctgtt aaggtgactt
     3301 ccatcaggtg cttccgattg agctcccttc attttccaaa gcatttcaga ttccttaccg
     3361 gcgatcattt tattgtatct ttctgaaaag atgctccaga actgaggcag gatggtttca
     3421 gcgatacttg ttgtcttttt caggtctgca tttgtggtat gcaatccgag aactgcacct
     3481 cttatccctt tttcaagcct tgtcatgtcc cagaaaagat gatatcctct aaaaaacctg
     3541 agtcgttctt tgtgtccaga attttcaaac ttacctgaaa atctcaagtt ctcatttttg
     3601 agcataaacc tgaaaaaact gataacatct ttatccacat tctgatcagt cggaaatatc
     3661 tgtaactcct cttcatattt atcccacaac accgcataaa caactgttcc tggcataaaa
     3721 ctctgaacag aagtgttaag ttttattatc atatcatcaa tatcagtcga ttttataagc
     3781 tcctttacta catcaagaat attcttgtat gcgtctttat ttgtaattgc attcatgata
     3841 acctccgttt acactaaaaa cggtagaaat atggcatttt atgaaaaaag tgtcaattta
     3901 tttaaaacag tttcatctgt tcgtgtctga tgtctgcttt gagtttgaaa cttttgcggt
     3961 atatttcgca gggtccgatc ttttcaagta tttcgtaatg ttcgcttgtt gcataacctt
     4021 tgtggaggtc gagtttatac tccggataaa tgagtgcaag ccggttcatt attctgtctc
     4081 tctgcgtttt tgcaaggatc gatgcggcgg cgatgttgac actttttgag tctcctttga
     4141 caactgcttc ttcaagatca aatttgcctg tcactctgtt tccgtctatc agaactatct
     4201 gttttccttc aacttttctt ttaactcttt caatgcactt agccattcca aacattgtcg
     4261 catgaagaat attaagttcg tcaatgattt ttggagaaac tgaatagact gcccagacac
     4321 tgttttttat aatttgcgga agaagttcaa gccgcttttt ttctgagagt ttctttgaat
     4381 cgttcagtcc ctcaatatcg cagaaaagac agaccgcggc agttacaacc ggtccgcata
     4441 aaggtcccct tcccgcttca tcaactccga tgactgaaaa gcccttgatc tgataatttc
     4501 tgtcgatttc gccaattaaa ccgttcattc cttaatacct tcaaacaatc tcagaagcac
     4561 atgatttaaa aaattcagtc ctttctcata gcaccttata acacttttat caatcactat
     4621 caactcttcc tcaacaagtt ctttaaccac ttttctttca aacatctgct caaatgatct
     4681 ttcaaatatc tcattgaatt tattgagttt tattccacta actactctaa gccccatgag
     4741 aagaaattcc attatcagct catttttgtc aggataatac cggcttaatc cgtcaccgtt
     4801 tatgaaagat tccagatctt caacataaaa actgtgttct cttttatttt caatgaacga
     4861 tttcgcccct gcaccaattc caagccatga atcaaaattc cagtaaagca tgttgtgaac
     4921 tgattctttc cctttttttg aatagttgct tatctcatat ttttcaattc ctctgttttt
     4981 caaaaattcc acgattgctt cttcctgctt catcgtttca tcttcatcga tcaacattgg
     5041 agcattcttt ttttcaggcg gagtatattg ataagcggaa acatggacgg ggttgacaag
     5101 tttaaaaaca ttttcaagtt cttctgtaat attgcggtct ttgccgatgc catagatcaa
     5161 atcaattgaa atgttgtcaa agttgtcatt tatcagtgga agtgcttctt tcatttgagc
     5221 aacactcacc cttctattca tctctttaag cacattatca tcaaatgact ggacacctgc
     5281 acttattctg ttcacccctg aattccttaa acttttaagc catttatcac taatatcagc
     5341 aggattgacc tcaactgtga tctccatctt tttaatgttg caacctagct cactgacagc
     5401 gtttaaaaga tctgaaaaga actcttcatc catcactgaa ggggttcctc cgccgacata
     5461 aagcgagaca atttcagtcg cgttaagttc atgaaaccgc tcaatcagtt cacttatcac
     5521 cttatcaccg taaattctca tgatctcgcg gtcttttatc acttcgctat aaaaatcaca
     5581 gtaactgcac cttttcacac aaaacggaat atgaatgtaa atccctgact ttttcatata
     5641 tcacctcaa
//
LOCUS       DAOOYB010000187         5636 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_16618_length_5636_cov_11.626411, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000187 DAOOYB010000000
VERSION     DAOOYB010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5636
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16618_length_5636_cov_11.626411"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(349..1137)
                     /locus_tag="PL056_10225"
     CDS             complement(349..1137)
                     /locus_tag="PL056_10225"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPM47431.1"
                     /translation="MKLWFFHIFVKFRWLISGLGSALLFMIFYFYVDGNIFATHSLCP
                     MAPVCTFFTFLRGEIFYFHGFLFFTVLVVFSLFVRRLFCFMFCPVGFVGDILFKIGRA
                     CRLPRISETNLFRSFSLYSRIFFLVSALFIPLFTGCMIFQHACPFILIGDMMYGIVVP
                     LAVSIFSVIVFLSLFISRFFCRFICPLGLIMGLTGRIGNRFLPTMTVDLTCKGGFSCG
                     KCTEICPTGINIAKLHGKIDDIDCILCGQCVEICALNRKKDEFH"
     gene            complement(1137..2513)
                     /gene="bioA"
                     /locus_tag="PL056_10230"
     CDS             complement(1137..2513)
                     /gene="bioA"
                     /locus_tag="PL056_10230"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013451176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HPM47432.1"
                     /translation="MYDRKKTLEVDRRNIWHPFTQMKDFENMDPKVIVRGEGVKLFDA
                     DGNEYYDTISSWWVNTLGHCNKEIAEGIKKQLDTLEHVNFSGFTHPYAAEFIDLLSGF
                     LDKSLTKYFFSDNGSTAVEVAIKMAFQYWQNRKINEKTRFIMLENAYHGDTIGAVSVG
                     GVEMYHKLYKPLMFNSIKVKSPDCSTCPKRKSEFTLDASKCDCNMECFADMAQAIKEN
                     AEHVAAIILEPLIQCAGGMFVYRKEYLQLLEKTAKENNVLVIYDEVATGFGRCGTMFG
                     YEQSGTHPDLICLSKGITAGFMPLAVTVATDEIYNAFYDDYFSHKTFFHGHSYTANPL
                     ACAAGIENLKILKRDHLPASNHIAMEHFHDRLTNLKKYDFINDIRYMGFIGAIDIVKS
                     RKDKVPFAPTDRTGKKIYETSLENGVVLRPLGDTIYFFLPLIVTVDDIDEIFNRAEKV
                     FNEAVGVY"
     gene            2736..3545
                     /locus_tag="PL056_10235"
     CDS             2736..3545
                     /locus_tag="PL056_10235"
                     /inference="COORDINATES: protein motif:HMM:NF018449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HPM47433.1"
                     /translation="MKKFLTASVFTVILIFSVSCSVEDDLSLPDDEVGSAVDNETELP
                     DAQIDDSNEFPDTNNNQPDNTPVPDESNLQPDDFDEQPDEPVIPTEMLPNNQWGTGGG
                     DGGKAVRVDSEGSIIVAGIAIGGLDGNLCVKQPDNQCSQDIFVSKWVGGTRIWTKQYG
                     TDKSEEANRIEIDPTGNIYIGGHSKGSIDGQTNAGNPLMATEDPFVSKFDSNGTKLWT
                     QLRGGNARDVAAGMALDSNGTVFLTGETYGAFPGTVSIGGRDIFLLYFLNQ"
     gene            3649..3951
                     /locus_tag="PL056_10240"
     CDS             3649..3951
                     /locus_tag="PL056_10240"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HPM47434.1"
                     /translation="MKSDTIFRIRNEVAFRKANDGTLTIVSPVTDKITTVNATASEIW
                     DMIDGKMSAGEIAEKFIRTHKDDNGFPGEKEAKNDVFAIISDFLNRELIEISGREK"
     gene            3948..>5636
                     /locus_tag="PL056_10245"
     CDS             3948..>5636
                     /locus_tag="PL056_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47435.1"
                     /translation="MNLSSFRFFTVALMFVLAAVLSGCSGKKTCSADTQCKDGSYCND
                     GKCTEFKEGDYKIVFESPSDKATITSAQDLDLSVEGIQIDVKVAMEDAQNYINDGMSV
                     ILSIKKGENEVLLTGKFLKNKAVFSQITLPEGEVTLSAYLEQNPGVKTTVTVNSVKMD
                     VTMYYLKGGAEGTKTLLESSIITDEDDSDGNLENGIQLNLVSETAGMKEGDIVNLFFP
                     QISEDNKAGEGNVDANGLVVFENITVPVLANVTMTVVSGEYSENVEFAVSSKIFCGFL
                     LNIEDDTIFGKKDDRNDTLSGLQYDLVISEITGCGKGSNVSIFIDSDETGTPWTIFTI
                     SSNQVEERITLQKSTSSEDKRSVLVVVEDEPKNMKGKKTAEGIIVDLDDPSVDISFPQ
                     VGAELNMSDDIDAGTPGLQINFAGEASDALTGPVSVEIKLGETVITTLEDINGDFESL
                     YTFPQSLPTAVLTVTVFDEAGNSSDAVIPFTVNIDPEIEFYSVCGHTGADIIDSMWLN
                     LSDDADENADLLQCKVVIKIGDGTGATHVSLKTGFGEDVKKEIESDNTAEFDISL"
BASE COUNT         1752 a         1088 c         1232 g         1564 t
ORIGIN      
        1 tttagaaaat tcaaattatt ttttgattta aaatgtatca gttttatgaa cattaaaact
       61 cccttttgaa atcaaggtgt tttaatagga tgtttgataa aacccttttt ataagtcggt
      121 tttatttaat catatctctc aaacgcctgt tcttaaacgg cttgcttaaa ttagaccttt
      181 ttgtctgatt tggtttttta taattttgct acaaggtcga ttgtcaaatt tattttattt
      241 cagaaaaaac agttggaaat tctgaagatt ctggaaggtg taaaagttaa gaatgccagt
      301 caatataata gcttgcaaga attgtcgtgg ggaatttgta ctaaatgttt aatggaactc
      361 atcttttttt ctattcaggg cacatatctc aacgcactgt ccgcagagta tgcagtcgat
      421 gtcgtcaatt tttccatgaa gtttcgcgat gttaattcca gtaggacaga tctcagtgca
      481 ttttccgcat gaaaaacccc ctttacatgt cagatctaca gtcattgtgg ggagaaagcg
      541 gttcccgatc ctgcccgtca gacccattat caatccgagc ggacagataa aacggcagaa
      601 aaatcgcgaa atgaaaaggg aaagaaagac gatcactgaa aaaattgata ccgccagagg
      661 aacaacgata ccgtacatca tgtcacctat caaaatgaaa gggcatgcat gctgaaagat
      721 catacaaccg gtaaacaagg ggatgaaaag tgctgagacc agaaaaaaga tccttgaata
      781 tagagagaat gaacggaaaa gattcgtttc tgaaatgcgc ggcaggcggc aggccctccc
      841 gatcttaaaa agaatgtctc caacaaatcc gacggggcag aacatgaagc agaaaagccg
      901 tctgacaaaa agggaaaaca caacaagaac agtaaaaaaa agaaatccgt gaaaatagaa
      961 tatctcacct ctgagaaaag tgaaaaaagt acataccgga gccattggac ataaactgtg
     1021 agtcgcaaaa atattcccgt caacataaaa atagaatatc atgaacagca acgcagatcc
     1081 cagtccagat ataagccacc tgaattttac aaaaatatgg aaaaaccata atttcattag
     1141 taaactccga cagcttcatt aaaaactttt tcagccctgt tgaatatttc atcaatgtca
     1201 tcaactgtaa caatgagagg aagaaagaaa tatatcgtgt cgccaagcgg tctgagtacc
     1261 actccgttct caagagaggt ttcgtatatc ttttttcctg ttctgtcggt cggagcaaat
     1321 ggaactttat ctttgcgact tttcacaatg tctatcgctc cgataaatcc catgtatcgg
     1381 atatcgttaa tgaaatcata tttcttgaga ttggtgagcc ggtcgtggaa gtgttccatt
     1441 gcaatgtgat ttgatgccgg cagatggtct cttttaagga ttttgagatt ttctattcct
     1501 gcggcacagg caagagggtt tgcagtataa gaatgtccgt gaaaaaatgt tttgtgcgaa
     1561 aagtaatcat cataaaaagc gttgtaaatt tcatcagttg caactgtaac ggccagaggc
     1621 atgaatccag cagtgatccc ttttgaaagg catatcaggt cagggtgagt cccgctctgc
     1681 tcatagccaa acattgttcc gcatcttcca aaacctgtcg ccacttcatc gtaaataaca
     1741 agcacattgt tctctttcgc agttttttca aggagttgca gatattcttt tctataaacg
     1801 aacattcctc cggcacactg gatgagcggt tcaagaatga ttgctgcaac atgttccgcg
     1861 ttttccttta ttgcttgcgc catatctgca aaacattcca tattgcagtc acattttgat
     1921 gcatctaggg tgaattcaga ctttcttttc gggcaggtcg agcagtccgg gcttttcact
     1981 ttgatcgagt tgaacatcag aggtttgtaa agcttgtgat acatctcgac tccgccgaca
     2041 cttactgccc cgatcgtgtc accgtgatat gcattctcaa gcatgataaa acgggtcttt
     2101 tcgtttattt tacggttctg ccagtactga aaagccattt ttatggcaac ttccaccgct
     2161 gttgagccgt tatcagaaaa gaagtatttt gtcagcgact tgtcaagaaa tccacttaaa
     2221 agatcaatga attcagccgc ataaggatgg gtgaagccgg aaaaattcac atgctcaaga
     2281 gtatcgagct gtttttttat cccttctgcg atctctttgt tgcagtgacc cagagtgttc
     2341 acccaccatg aagaaatagt gtcataatat tcattgccgt cagcatcaaa aagtttcacc
     2401 ccttcacccc ggacgatcac cttcggatcc atattttcaa aatccttcat ctgtgtgaac
     2461 ggatgccaga tattccgcct gtcaacctca agagtctttt tcctgtcgta catgatccac
     2521 cttgataaaa aaacaatcac ctgacgaata tcacgcataa aaaacatttg tcaaggttac
     2581 tgcgagtttc tatatttgcg tgcattaatc aacttcccaa aagtccgcaa ttaagaaata
     2641 aggacgtact gataaaagaa aatttcaaat aaaggacgag ataaaagtat gtttttaata
     2701 actttttgaa gtttttttaa cttcaggagg attttatgaa aaagtttcta acagcttcag
     2761 tttttactgt cattttaatt ttttctgttt catgttccgt tgaggatgat ttatccctgc
     2821 ctgatgatga ggtcggttct gccgttgaca atgaaactga acttccggat gcccagattg
     2881 acgacagtaa tgagtttcca gatacaaaca ataaccaacc tgacaacaca cctgttcctg
     2941 atgagagcaa tctgcagccg gatgactttg atgaacagcc ggatgagccg gtgattccga
     3001 cagaaatgct cccgaacaat cagtggggaa ccggcggagg agacggtgga aaggcagtca
     3061 gggttgacag cgaagggagc attattgttg cgggaattgc aatcggaggg cttgacggaa
     3121 acctgtgtgt caagcagcct gacaatcagt gttctcagga tatttttgtt tcaaaatggg
     3181 tcggagggac cagaatctgg acaaaacagt acggcactga caaaagcgaa gaggccaaca
     3241 gaatagaaat cgatccgacc ggaaacattt atatcggagg tcattccaaa gggagcatag
     3301 acggacagac caatgcagga aatcctctca tggcaaccga agatccgttt gtttcaaagt
     3361 ttgacagcaa cggaaccaaa ctctggacac agcttagagg gggaaatgcc agagatgtag
     3421 ctgccggaat ggcgcttgac agcaatggta ccgtcttttt aactggcgaa acctatggag
     3481 catttcccgg aactgtttca ataggcggca gggacatttt tcttctctat tttcttaatc
     3541 agtaaattta tcttaccgac ctggtcaatc caaacaaaaa atttgatatt atttatttaa
     3601 ggattatcat ttcaatctga aattgtatta cagttgaagg gggaagattt gaaaagtgac
     3661 accattttca ggattaggaa cgaggttgcg ttcaggaagg caaatgacgg tacgctgact
     3721 atagtttcgc ctgttaccga taaaattacg actgtcaacg ctacggcttc agagatctgg
     3781 gatatgatcg atggaaaaat gagtgccgga gaaattgctg aaaagttcat ccggacacat
     3841 aaagatgata acggtttccc gggagagaaa gaagcaaaaa atgatgtttt tgcgataatc
     3901 agtgattttt tgaatcgtga attaattgaa atatcaggga gggaaaaatg aacctgagca
     3961 gttttagatt ttttacagtt gccttaatgt ttgttcttgc cgctgttctt tccggctgtt
     4021 ccggaaaaaa gacatgcagt gccgataccc agtgcaagga cggctcatat tgcaatgacg
     4081 gaaaatgtac agaattcaaa gaaggtgatt ataagatcgt ttttgaaagt ccgtcagaca
     4141 aagcaacgat aacaagtgcg caggaccttg atctttctgt agaagggata cagatagatg
     4201 ttaaagtcgc aatggaagat gcgcaaaact atatcaacga cggaatgtct gtcatattaa
     4261 gcattaaaaa aggtgaaaat gaagtcctgc tcacagggaa atttttgaaa aacaaagcag
     4321 tcttttcaca gatcaccctt cctgagggag aagtgacgct ttcagcatat cttgaacaga
     4381 atccgggagt taagacgact gtcaccgtca attcagtgaa aatggatgtg acaatgtatt
     4441 accttaaagg tggcgcggaa gggacaaaga cactccttga aagttctatc ataacagacg
     4501 aagacgattc tgacggaaac ctcgagaacg gtattcagct taaccttgtt tctgaaacgg
     4561 cagggatgaa agaaggtgat attgttaatc ttttctttcc gcagatcagt gaggacaata
     4621 aggcaggcga aggaaatgtc gatgccaacg gcttggttgt ttttgaaaat atcacagtcc
     4681 ctgttcttgc gaatgttaca atgacggtcg tttcaggcga atattcggaa aatgttgaat
     4741 ttgcagtcag ttcaaaaata ttctgcggat ttcttttaaa tattgaagat gacactattt
     4801 tcgggaaaaa ggacgatagg aatgacactc tttccggact gcagtatgat cttgttataa
     4861 gtgaaataac gggatgcggc aaaggcagca atgtttccat tttcattgat tccgatgaga
     4921 cgggaacccc atggacaatc tttacgataa gttcaaatca ggtcgaagag aggatcacac
     4981 tccagaaaag tacatcatct gaagataaga gaagcgttct tgttgtagtt gaagatgagc
     5041 cgaaaaatat gaagggcaag aaaacagctg aagggataat cgttgacctt gacgatccgt
     5101 cagttgacat ttccttccct caggtcggtg ctgaattgaa catgagtgat gatattgatg
     5161 ccgggacgcc gggtcttcaa atcaattttg caggcgaggc atcggacgca ctgacaggtc
     5221 cggtatctgt cgaaattaag ctcggagaaa cagttatcac gacgcttgaa gacataaatg
     5281 gtgacttcga gtctctttat accttcccgc agtctcttcc gacagctgtt ctgaccgtaa
     5341 cggtttttga tgaggcgggc aactcaagtg acgcagttat tccttttact gtcaatatcg
     5401 atcctgagat agaattttat tcagtttgcg gtcataccgg agcggatata attgattcga
     5461 tgtggcttaa tctcagtgat gatgctgatg aaaatgccga tctgcttcaa tgtaaggtcg
     5521 taattaaaat cggtgatgga acaggagcaa cacatgtttc tctcaagacc ggtttcggcg
     5581 aagatgtcaa aaaagagata gaatctgaca acaccgccga atttgatatt tctctt
//
LOCUS       DAOOYB010000188         5571 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_16892_length_5571_cov_6.388869, whole genome shotgun sequence.
ACCESSION   DAOOYB010000188 DAOOYB010000000
VERSION     DAOOYB010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5571
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16892_length_5571_cov_6.388869"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2026
                     /locus_tag="PL056_10250"
     CDS             <1..2026
                     /locus_tag="PL056_10250"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HPM47436.1"
                     /translation="VDKVFVKYFSENGSSVKAFNTMPRWVYFYVDSMPKEALLPGDPD
                     YDGIDPEANPSFVVPPEYYVSPTLKYVVQAYINKNIDYLTEKLIVEGQTNRMASEIAT
                     YFEMTPETEKTQLRFNGKELEEGLLVSYVPAQNSGNLMNRAMDPIWATYFSQIILPMI
                     MISADVNDNFLKILTNYDKTVNKGLSSHPVHVVASGIRKRGIGQRVNDFMKSRKQSFG
                     TPLYKSDLYIKVMEVIVNSFSGETKSSRAFLEELKEVTGSETYVDQLQKITDLLFRKL
                     APVDIIKLFSGMDAPIIEFTDEVFNFGNTGEDVYYFNEVIEEETDTETTDEEYNPYPT
                     EEDVCAKEKCLCLPESGFNTSEFTVGDKPGCMLQVAAGNKMFLMGSDSDDSFVMEKPS
                     HWITINDNFLIDKYEVTVSQYKRFLNDISNKPWLPENAAKTDDELGLEKCYGNDKYLE
                     EWFGEFSGDDFMGPSKDKMPVTSVCWYAANAYCKWAGKRLPTEEEWEYAAKVSNDCIC
                     APGDTACIETGKKCPEMPWGTGFWDSINISYQANYRNSNDPFEPSRELEDLLSPEDLL
                     KAYPEPHVTPVGYFNGNKYDAFNSKDGKSPSGVYDMSGNAEEWVGTRFFYYSDLFQGG
                     VPLPIGDQRTVRGGSWKTSRRLIRATFRRGVNPQYSSNSVGFRCAMDITQ"
     gene            2041..3366
                     /locus_tag="PL056_10255"
     CDS             2041..3366
                     /locus_tag="PL056_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47437.1"
                     /translation="MSKMIKTIMPVLLILFSVNVFAEIKQEGDEWRVYDEQYSEEIEF
                     EYRDVLIEHQKWGTGDWLPGCGGLMPLSVKFNVEYGAEMGAKMKGRAHAKWPEAVKVY
                     FEGTPNGGEADMFYGINMEALWKNCYSGQEQELSKWLNFDLSFQDYKIYEPFLLDGNV
                     DNPVSMEDSIDHFLIYEGTLADMVGGSCGLSIPFIGDICSLIHFKFELDGKMYVEIFG
                     SRIALDEKQGRSIFSDDQYLVIEPPLVGTIMEIPVHYESKVYYETIIIFTITIRIRIL
                     ELEFPIPIEIPIADGDKIWTYNSQTVYFNFPAIFIDRRSHKFADTVPGDEDYWEFEVE
                     NIGSRPLEGKIISDNSNFIVMPDTVNLDPGGSTTVTVFFKPYDVGTKKGTIKFYSNDP
                     VEPYTTVSLFGYSSESDTQWTKVIEGDEPRWEVVSKGNGCSVSVIGGEQ"
     gene            3363..4652
                     /locus_tag="PL056_10260"
     CDS             3363..4652
                     /locus_tag="PL056_10260"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HPM47438.1"
                     /translation="MKRSFSVIPLLIFAIMFSGCTSGGEYMFQKTGQDFDEKGGIYKG
                     VDDVTVHIPEGALPEGIDKITITVYALDTAPFTNVSKPVTNIYSISPAAIEFTKDLTV
                     VIPYKEKSFPNLLDELDLKPYFSLDRQSFEVFDKEVYQLDVENNIIKISTKYLGNFHL
                     GFPKELVKQEDSDPSGIAEMILVPAGDFQFGAPQGTKTGVAENEEGMKESEGLNTVYL
                     SAYYIDKYEVTNRQYQLCQEAGVCVEPHDLTSVMYQNYYYNPNYAGFPVIWVSYNQAL
                     TFCHWAGKTLPTEAQWEKAARGETFTEYPWGKEPPADNIEATVANYSALFGDVTNATW
                     GEDGVSKYGVYNMAGNVAEWTDTWHNIDSYYQKRTDGVIVEDPIGPADSPTQEKVIKG
                     GSWVSLENEITTYARDKAFPNYRYYNLGFRCVQSVNK"
     gene            4670..>5571
                     /locus_tag="PL056_10265"
     CDS             4670..>5571
                     /locus_tag="PL056_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47439.1"
                     /translation="MKKVHILIVIFMALSGFYSVSCDKSDPPENDDPALNDEDAPVNE
                     GQTEVPDNAEDCNYSSADNRLRPKNEEVFFSGIIFAEQSLTKYTYVMNRCYNCSDSSP
                     LIITAVSIVNENGVKDEGGFKIESNPVSGGPVSLKNDEEVGIGISVVASNWDEQVRYL
                     RITSNDKCKPNFDIKLIAQTKPTGKIVVKTIDDAEPDDEVMVFKDVLDELVNRVQVFN
                     EGTANLKLLAVSISQGKSKNANETGFFISHAPEPNVQIAPADSMMLMIGCRNDKEYPQ
                     PLTGELLIVNNDPTEYGINQDKKIV"
BASE COUNT         1697 a         1074 c         1337 g         1463 t
ORIGIN      
        1 ggtcgacaag gtctttgtaa agtatttcag cgagaacgga tcatctgtaa aagctttcaa
       61 cacaatgccg agatgggtct acttctatgt tgactcaatg cctaaagaag cgcttctgcc
      121 tggagatccc gattacgacg gaattgatcc tgaagctaac ccttcttttg ttgttccgcc
      181 tgaatattat gtttctccga cactgaaata tgtagttcag gcatatatca ataaaaatat
      241 tgattatctt accgaaaaac tcattgttga aggtcagacc aacagaatgg cttcagagat
      301 agcgacatat tttgaaatga ctccggaaac cgaaaaaacg cagttgagat tcaatggcaa
      361 agagctggaa gaaggtcttc tcgtaagcta tgtgcctgct cagaacagtg gaaatcttat
      421 gaatagagcc atggacccga tctgggcaac ttacttttcc cagatcatcc ttcccatgat
      481 catgatctct gctgatgtca acgacaactt cctgaaaata ttgactaatt atgataaaac
      541 tgtcaacaag ggactttcgt ctcacccggt tcatgtcgtt gccagcggaa taagaaaaag
      601 aggtatcggt cagagggtca atgactttat gaaaagcaga aaacagtctt tcggaacacc
      661 tctgtataaa agtgatcttt acataaaagt catggaggtc attgtcaatt cattctccgg
      721 tgaaactaag agcagcagag cttttcttga ggaactgaag gaggttaccg gcagtgaaac
      781 ttatgtcgat cagttgcaga agataaccga tcttttgttc agaaagcttg cgccggttga
      841 tataattaaa cttttctccg gaatggatgc accgataatt gaatttaccg atgaagtgtt
      901 caatttcggc aacacaggcg aggatgtcta ttattttaac gaagtgatcg aagaggaaac
      961 tgatactgaa actactgatg aggaatacaa cccttatccg acagaagaag atgtctgtgc
     1021 aaaggaaaaa tgtctgtgtc ttcctgagag cggatttaac acatcggaat ttactgtcgg
     1081 ggataagccg ggatgcatgc ttcaggttgc agccggcaat aaaatgttcc tcatgggatc
     1141 tgattcagat gattctttcg ttatggaaaa accatcccac tggataacga taaatgataa
     1201 ttttcttatt gacaaatatg aggtcacagt ttcacaatac aagcgtttcc ttaatgatat
     1261 cagcaacaaa ccctggcttc ctgaaaatgc tgcaaagact gatgacgagc tcggtcttga
     1321 gaaatgttac ggtaacgaca aatatcttga ggaatggttc ggagagttca gcggagatga
     1381 ctttatgggt ccttcaaagg ataaaatgcc ggttacaagt gtctgctggt atgctgcaaa
     1441 tgcgtactgc aaatgggcag gaaagcgtct tccgactgaa gaggaatggg agtatgccgc
     1501 aaaagtttca aatgattgta tctgtgctcc cggagatact gcatgcattg aaacgggtaa
     1561 aaaatgtccc gaaatgcctt ggggaaccgg tttctgggac agcattaata tttcctatca
     1621 ggcgaattac agaaacagta atgatccttt tgagccgagc agggagcttg aagatctcct
     1681 ttcccctgaa gatcttctga aggcatatcc tgagccgcat gtaactcctg taggatattt
     1741 caacggcaac aaatatgatg cattcaattc caaggatggt aaaagcccga gcggtgttta
     1801 cgatatgtcg ggaaatgctg aggaatgggt aggaacgaga tttttctatt actccgacct
     1861 tttccagggc ggagttcctc ttccgatcgg tgatcagaga acagttcgcg gaggaagctg
     1921 gaagacatca aggcggctta tcagggcgac attcagaaga ggtgtcaacc cgcaatatag
     1981 ttccaactct gtcggtttca ggtgtgcaat ggatataacg caataatata aggtgcttaa
     2041 atgagtaaaa tgatcaaaac aatcatgccg gtcttactga tccttttttc cgttaatgtt
     2101 tttgcggaaa taaagcagga gggtgatgaa tggcgagtct atgatgagca gtattctgag
     2161 gagatcgagt tcgagtaccg tgatgtactt attgaacatc agaaatgggg cacaggtgac
     2221 tggcttccgg gatgcggagg gctgatgcct ctcagtgtca agttcaatgt tgagtacggt
     2281 gctgaaatgg gtgcaaaaat gaaaggccgt gcccatgcga aatggccgga agcggtaaaa
     2341 gtatattttg aaggcactcc aaacggtggt gaagccgata tgttctacgg aataaacatg
     2401 gaagctctct ggaagaattg ttatagcgga caagagcagg aactcagtaa atggcttaat
     2461 tttgacttaa gctttcagga ctacaagatc tatgaacctt tccttcttga cggaaatgtt
     2521 gacaaccctg tttcaatgga ggacagcatt gaccatttcc tgatatatga agggactctg
     2581 gctgatatgg tgggcggatc atgcggactt tccatacctt tcatcggcga catatgcagt
     2641 ctgatccatt tcaagttcga acttgacgga aaaatgtatg ttgaaatatt cgggagcagg
     2701 atcgcccttg acgagaaaca gggtcgatcg atcttttcag acgaccagta tttagttatt
     2761 gagccgcctc ttgtgggaac cataatggaa ataccggttc attatgaatc gaaggtctat
     2821 tatgaaacga tcataatctt cacaataaca ataagaataa ggatccttga acttgaattt
     2881 cctattccaa tcgaaattcc gattgccgac ggtgacaaga tatggacata caattctcaa
     2941 actgtttatt tcaactttcc ggcaattttc atagacagaa gaagtcataa atttgccgac
     3001 acagttccgg gggatgagga ttactgggag tttgaagtgg aaaatatcgg atcaagacct
     3061 cttgaaggaa agatcatttc agacaactcg aatttcattg taatgcctga tactgtcaac
     3121 ctcgatccgg gtggaagcac tacagtgact gttttcttca agccatacga tgtcggaaca
     3181 aagaaaggga caataaaatt ttacagcaat gatcccgttg agccgtatac gaccgtttct
     3241 cttttcggat acagcagtga aagcgacacc cagtggacaa aggtcattga aggtgacgag
     3301 ccgagatggg aagtcgtttc aaaaggtaac ggctgttccg taagtgtgat cgggggtgaa
     3361 caatgaaaag atcgttttct gtcatccctt tactgatttt tgcgatcatg ttctccggct
     3421 gtacaagcgg aggagagtat atgttccaga agacgggaca ggattttgat gaaaaagggg
     3481 ggatttataa aggtgtcgac gatgttacgg tccatattcc tgaaggtgct ctgcctgaag
     3541 gaatagataa gataacaata acggtctatg ctcttgacac agcaccattt accaatgttt
     3601 ccaaacccgt tactaacatt tattcgatct cacctgcggc aatagagttc acgaaagacc
     3661 tcaccgtggt cattccatac aaggagaaat catttcccaa tctgctggat gaacttgacc
     3721 tgaaacctta tttctcgctt gacaggcaga gttttgaagt ttttgacaaa gaggtctatc
     3781 agcttgatgt cgagaacaac atcatcaaga taagcacaaa atatctcggt aatttccatc
     3841 tcggttttcc gaaagaactt gtaaaacagg aagattccga tccttccgga atagctgaaa
     3901 tgatccttgt tccggcaggt gatttccagt tcggtgcacc tcagggaact aagaccggag
     3961 tggctgagaa cgaagaaggg atgaaagagt cagaagggct taacaccgta tatctctctg
     4021 cgtattacat tgataaatat gaggtgacaa acaggcagta tcagctttgt caggaagccg
     4081 gtgtctgtgt cgagccgcat gaccttacct ctgtaatgta ccagaattat tactataatc
     4141 cgaattatgc cggatttccg gtaatatggg taagttataa tcaggcgctt acattctgtc
     4201 actgggcagg gaaaacactt ccgacagaag cacagtggga aaaagcggca agaggggaaa
     4261 cttttacaga atatccctgg ggtaaggagc cgccggcaga caatattgaa gctacagttg
     4321 caaactattc agcgcttttc ggtgatgtta caaatgcaac atggggcgaa gacggtgttt
     4381 caaagtacgg tgtctataat atggcaggaa atgtggccga gtggactgat acatggcaca
     4441 acattgacag ctactaccag aaaaggacgg acggagtgat tgttgaagat ccgatcggtc
     4501 ctgccgattc gccgacacag gagaaggtga tcaaaggtgg gagctgggtc tctcttgaaa
     4561 atgagataac aacatatgca agggacaaag cattcccgaa ttacagatac tataatcttg
     4621 gattcagatg tgtacaatcg gtaaacaaat gatgagcttg gagataaaaa tgaaaaaagt
     4681 gcatatttta attgtcatct ttatggcact ttccggtttt tattcggttt cctgtgataa
     4741 gtcagatccg ccggaaaatg acgatcccgc acttaatgac gaagatgccc ctgttaatga
     4801 aggccagacg gaagtgcctg acaatgctga ggattgtaat tattcgagtg ctgataacag
     4861 acttagaccg aaaaacgaag aagtgttctt ttcaggtata atatttgctg agcagtcgct
     4921 gactaaatac acttatgtca tgaacaggtg ttacaattgt tcagacagtt ctcctctcat
     4981 aataactgcg gtttcaatag tcaacgagaa cggagtgaag gatgagggcg gattcaagat
     5041 cgagagcaat ccggtttcag gaggtcctgt aagtctgaaa aatgatgaag aggtcggcat
     5101 agggatctct gttgtagcca gcaactggga cgaacaggtg agatacctca gaataacaag
     5161 caacgataaa tgtaaaccca attttgacat taaacttatt gctcagacaa aacctaccgg
     5221 caaaatagtg gtgaaaacta ttgatgatgc tgagccggac gatgaagtga tggttttcaa
     5281 agatgttctt gatgaacttg tgaacagagt acaggtgttt aatgaaggga cagccaacct
     5341 taagcttctt gccgtttcca tatcgcaggg gaagtctaaa aatgcaaatg agaccggttt
     5401 cttcatttca catgctcccg agccgaatgt tcagatagct ccggcagact ccatgatgct
     5461 catgatcggt tgcaggaatg acaaggaata tcctcagccg cttaccggag aacttcttat
     5521 cgtgaacaac gatccgacag agtacgggat aaatcaggat aagaaaatag t
//
LOCUS       DAOOYB010000189         5556 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_16954_length_5556_cov_10.499182, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000189 DAOOYB010000000
VERSION     DAOOYB010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5556
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16954_length_5556_cov_10.499182"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..853)
                     /locus_tag="PL056_10270"
     CDS             complement(<1..853)
                     /locus_tag="PL056_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47440.1"
                     /translation="MFNLQKAMLICSVMLLMLSVFSCTSDKKTQIYPDDNGNINTDND
                     SAGIDDSGLPDAGNTGDSGNTGNTGDTGDTGNTGDTGNTGNTGDTGNTGNTGNTGDTG
                     NTGNTGNTGNTGDTGNTGDTGDTGNTGDTGNTGDSGCTPGTKYPCYTGSSDTRNKGIC
                     KDGEAECLEDGVSFGECLNEVLPDPAGEICYNSLDDNCSGAENEGCICVPNAIEACYE
                     GPFGTENVGDCKPGEMTCSADGRSYSSCLNQVLPGAELCDSGHDENCDGTQMTAGGTG
                     DPNEDGDA"
     gene            989..2899
                     /locus_tag="PL056_10275"
     CDS             989..2899
                     /locus_tag="PL056_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47441.1"
                     /translation="MKSLFTVATIIALVFFFGCSEKKKSEADIDGQSNADSDLEEDTD
                     EVEKTDVEPDGPEIITENENEQTDAEPDEEVKDTETTDDIVTDSEPDEENDEDVVEPL
                     PSVIYVNESAEGAEDGTSWENAFMDLQSAIDAAVEGDQIWVAAGKYKPTSVHGIITME
                     DVYDIDEFNRYLHFRMKNGVEIYGGFAGTETELSQRDFQAQETILSCDLGEIDEQYDN
                     CFHIFYHPAGLDLDETAVLDGFLMEGGVADETEPHDAGSVMYNDGSSPAVKNGWIYWN
                     YSYKGAVFYNKDSSMKVMDTQFVNNEAPYSKGGVFYNENSSIEISGCDFANSWAEGDN
                     ESNGGAIYSTGGKTVIADTIFRSNEANLGGAIFVESGDLEISGCNFEENKGEFGAAIY
                     LKSANCVISKSDFRYNDTYSYGETDGRGAIYLIDSELKVTDSIFYKHISGWGGAIYST
                     SATDGGSKLTIINSAFSVNKMNEQGGALYLNKTNTDIVNSVFFKNYWGYSSTGTSGGA
                     IYRANNGSLTVTNSIFKSNTAGTGKHIHDAAGNATVTYSCIVDTTVYPGTGNINDEPL
                     MISPEYNPLNFGIQPESPCIDTGSNTPFDAGNIAEGITKDMSGNDRIIKGKETSPSAI
                     VDMGAVEFKPAP"
     gene            2910..3476
                     /locus_tag="PL056_10280"
     CDS             2910..3476
                     /locus_tag="PL056_10280"
                     /inference="COORDINATES: protein motif:HMM:NF012330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine phosphatase family protein"
                     /protein_id="HPM47442.1"
                     /translation="MPTKIIRNSYWVVPGEILAGPFPGSDEEFSYKLRLRALFDVGIR
                     AFINLQTEGEMASSSRDYDEDYGKIFRHFLLQNNEKEIDGGFIRRFSIPDREIPSIEL
                     MINIINEIDMLRSRNIPIYIHCWGGIGRTGTVVGCWLMRHGFAERRNVIDEIFNLRKL
                     YVETSCLVYRSPETEQQEQFILNWEKGQ"
     gene            3478..>5556
                     /locus_tag="PL056_10285"
     CDS             3478..>5556
                     /locus_tag="PL056_10285"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HPM47443.1"
                     /translation="MSRIYISPDKPLRPKIIPVMDVYDIPHLTDEESARYRELKTIVK
                     NIKAHENSTNPEITGNIENFYRTQLNPMQCEAVFQLVGAILVIAGAGSGKTRTIVHRV
                     AYMLQKGIEPQSILLLTFTRRASGEMIKRVNELTNGETADKITAGTFHSFANMMLRRY
                     GRFACIEPGFTICDTVDSADIIDLIKNDLDIKKRSRPFPKKATVFEIISRCRNHNRPI
                     GEILDSQYPKYLEFAEDIVKIAAKYSEYKRAKNVLDYDDLLERFLELLITNSEVREKI
                     QSKYRFVMVDEYQDTNTFQGQIADIIAKEHGNILVVGDDLQSIYAFRGANFENILRFP
                     QNWPQCRVIKLEQNYRSIRELLDFTNNIVENCYAAYNKVLFSEMSGAVKPFVKRAIDA
                     ESEAVFVAEKIEKALENIEPDEIAVLYRSGFHGNFIQAELLKRGIDFAVYGGIKFIER
                     RHVKDVLSYAKMIENPKDAVALNRILKLIPGIGSGTAARIVESVVENGFIEAETHKKK
                     KYYEEIKKLFSLISSITEEKPDVLKTVRIITDFYAPILKTIESDFEERLRDIDVLIQI
                     AKNYRSLEKFLTDFALDPPSTQINMLATPVDAAQISKKVILSTVHSAKGLEWNTVFVV
                     HLLDGCFPSERSLKRIDDLEEERRLFYVACSRAKEKLYLTFPNTLSFYGGNLSLPSRF
                     IAEIDEKFYEI"
BASE COUNT         1730 a         1136 c         1176 g         1514 t
ORIGIN      
        1 atgcatcacc atcttcattg ggatcacctg ttccgcctgc tgtcatctgc gttccatcgc
       61 aattttcatc atgtccgctg tcacaaagct ctgcacctgg aagaacctga tttaaacatg
      121 aactgtaact tcttccatcg gcagaacatg tcatttcacc cggcttgcag tcaccgacat
      181 tttcagttcc gaaaggacct tcataacaag cttctattgc gttcgggaca caaatacatc
      241 cttcattttc cgcaccgctg cagttgtcat caagtgagtt gtagcagatc tctcccgccg
      301 gatccggcag cacttcattc aggcattcac caaatgaaac tccatcttcc agacattctg
      361 cttcaccatc tttgcatatt cccttatttc ttgtatcgga acttccggta taacacggat
      421 atttcgttcc gggagtacag cccgaatcac cagtgtttcc ggtatcacca gtgtttccgg
      481 tatctcctgt gtcaccggta ttgcctgtgt caccagtgtt tccggtattc cctgtgtttc
      541 ccgtgtttcc tgtgtcacca gtgtttccgg tattcccagt gtttccggta tcaccagtgt
      601 ttccggtgtt tccagtatct cctgtgttgc ccgtatcgcc ggtatctcct gtatttcctg
      661 tattaccgct gtctcctgta tttcctgcat ccggaagtcc tgaatcatca atgccggctg
      721 aatcattgtc cgtatttatg ttcccgttgt catcagggta tatttgtgtc tttttatctg
      781 aagtacagga aaaaactgac aacatcaaca acataacact gcaaattaac atcgcttttt
      841 gtaaattaaa catcactcat cctcccgata agaaacataa tgaaataatt ttaggtcttt
      901 taagaaaaat gtcaatcatt atccttttta tttacttttt tacctaaaat ctgtataaat
      961 ctgtgtttac catcattgga ggaataaaat gaaaagtctt tttaccgttg cgacaattat
     1021 cgcacttgtt tttttcttcg gctgttcaga aaagaagaaa tctgaagctg atattgacgg
     1081 acagtcgaac gctgattcag atttggagga agacaccgac gaagttgaaa aaacagatgt
     1141 cgaaccggat ggaccggaaa taatcactga aaacgaaaat gaacaaacgg atgcagaacc
     1201 tgatgaggaa gtaaaagaca ccgaaacaac cgatgatatt gtgacagata gcgagcccga
     1261 cgaagaaaac gatgaggatg tcgttgagcc ccttccttct gtcatatatg tcaatgaatc
     1321 tgcagaaggt gcagaagacg gaacaagctg ggagaatgct ttcatggacc ttcaatcggc
     1381 aatcgatgcg gctgttgaag gtgatcagat atgggttgcc gccggcaaat acaaacccac
     1441 ttcagttcac ggaattatca caatggaaga tgtttatgac atcgatgaat tcaacagata
     1501 tctgcacttc aggatgaaaa acggcgttga gatctacggc ggttttgccg gaacagaaac
     1561 ggaactttcc caaagagatt ttcaagcgca ggaaacaatt ttaagctgtg atcttggtga
     1621 aattgatgaa cagtatgaca actgttttca tattttctat catcctgccg gacttgatct
     1681 tgatgaaact gccgttcttg acggttttct gatggaggga ggagttgctg atgaaactga
     1741 gccgcatgat gcaggaagtg tgatgtataa cgacggaagc agtcccgcag tgaaaaacgg
     1801 atggatctac tggaattact catataaagg ggctgttttc tataacaagg acagctcaat
     1861 gaaagtaatg gacactcagt ttgtcaacaa tgaggcacct tattcaaaag gcggtgtttt
     1921 ttataatgaa aacagcagca tcgaaatatc gggctgtgac tttgcaaaca gctgggcgga
     1981 aggagacaat gaatcaaacg gcggtgcaat ttacagcaca ggcggaaaaa cagttattgc
     2041 agacacaatt ttcagaagta atgaggcaaa tctcggcgga gcaatttttg tggaatctgg
     2101 cgatctggaa atttccggct gtaattttga agagaacaaa ggtgaattcg gtgcggcaat
     2161 ttatctaaaa tcagctaact gtgttatttc aaaaagtgat ttcagatata acgacacata
     2221 cagttatgga gaaactgacg gcagaggtgc tatttatctg atcgattctg aattgaaagt
     2281 gacagactca atcttttaca agcacatttc tggatggggc ggagcaattt attcaacttc
     2341 agcaacagat ggcgggtcaa agctcacgat catcaactcg gcgttttcag tcaataaaat
     2401 gaatgagcag ggcggagcat tatatctgaa caaaacaaac actgacatcg tgaactcggt
     2461 gtttttcaaa aattactggg gatactcctc aaccgggaca tcgggcggag ctatatatag
     2521 agcgaacaat ggttcattga cagtaacaaa ttccatattc aaatcaaaca cagcgggaac
     2581 tggaaaacac attcacgatg cggcagggaa tgcgaccgta acatactcat gtattgttga
     2641 cacaactgtt tatcctggca caggcaacat aaacgatgaa cctttaatga tttcaccgga
     2701 atacaatccg cttaatttcg gaattcagcc agaatctccc tgcattgata ctggctcaaa
     2761 cacccctttt gatgctggaa atatcgctga aggtatcaca aaagacatgt cgggcaacga
     2821 ccggataata aaaggaaaag aaacttcacc ttcagcaata gttgacatgg gtgccgttga
     2881 attcaaaccg gctccataaa aaaataacga tgcctacaaa aattatcaga aacagttact
     2941 gggtcgtgcc tggcgaaatt cttgcgggtc cgtttccagg aagtgatgag gaattttcat
     3001 ataaactgcg gctccgagct ttatttgatg taggaataag ggctttcatc aatcttcaga
     3061 cagaaggtga aatggcttca agcagtcgcg attatgacga agattacgga aaaatattca
     3121 gacatttttt acttcagaac aatgaaaaag agatagatgg cggttttatc cggagatttt
     3181 caattcctga cagagagatc ccctcaattg aactgatgat aaatattatt aatgaaattg
     3241 acatgttacg atcaagaaac attcccattt acattcactg ctggggaggc attggaagaa
     3301 caggaacagt tgtcggatgc tggctaatga gacatggatt tgcagaaaga agaaatgtca
     3361 tcgatgaaat attcaattta aggaaactgt atgtcgaaac ttcctgcctt gtttaccgtt
     3421 caccagaaac ggagcagcag gagcagttca tcctgaattg ggaaaaaggt caatgaattg
     3481 agcaggatat atatctctcc agacaaacct ttaagaccaa aaattattcc tgtcatggat
     3541 gtttacgaca ttcctcatct gaccgatgaa gagtcggcaa ggtacagaga acttaagacc
     3601 attgtaaaaa acatcaaagc tcatgaaaac agtacaaatc cggaaattac aggcaatatc
     3661 gaaaattttt acagaactca gcttaatccg atgcagtgtg aagcggtttt tcaacttgtt
     3721 ggggcaatac tcgtcattgc aggggcagga agcggaaaaa caaggacgat cgtccaccgt
     3781 gttgcctata tgctgcaaaa agggatcgag ccacagtcaa ttctgctact tacatttacc
     3841 cgccgtgcat cgggagagat gataaaaagg gtcaatgaac ttacaaacgg tgaaactgcc
     3901 gataaaatta ccgcaggaac ttttcacagt tttgccaata tgatgcttcg aagatatggc
     3961 agattcgctt gtattgaacc ggggttcacg atctgtgaca ctgttgattc ggcagatatc
     4021 attgatctta ttaaaaatga tctcgatata aaaaaaagat cccgtccatt ccctaaaaaa
     4081 gcaactgttt ttgaaataat cagccgatgc cgcaatcata accgtccaat cggtgagatt
     4141 cttgactctc aatatccgaa atatcttgaa tttgctgaag atattgtaaa aattgccgca
     4201 aaatattctg aatataaaag agcaaaaaat gtgctggatt acgatgatct tcttgaacgg
     4261 ttcctcgagt tgctgatcac gaactctgag gtcagagaaa agattcagtc aaaataccgt
     4321 tttgtgatgg ttgacgaata tcaggacact aacactttcc agggtcaaat cgccgatatc
     4381 attgcaaaag aacatggaaa tattcttgtt gtgggtgatg atcttcaaag catttacgct
     4441 ttcagagggg caaatttcga gaacattctg cggtttcctc agaactggcc gcagtgtcgg
     4501 gtcataaaac ttgagcagaa ttaccgctcg atcagagaac tgctcgattt cactaataat
     4561 atcgttgaaa actgctatgc cgcatataac aaggttcttt tttcagaaat gtcgggggcg
     4621 gtcaaaccat ttgtaaaaag ggcgattgac gctgaatctg aagcggtttt tgttgctgaa
     4681 aagatcgaaa aagcactcga aaatatagag ccggatgaga tcgcagttct ataccgctcc
     4741 ggctttcacg gcaatttcat tcaggcggaa cttttaaaaa gaggaataga tttcgcagtt
     4801 tacggcggaa taaagttcat tgaaaggcgc catgtcaaag atgttttaag ttatgcaaaa
     4861 atgattgaaa atccgaaaga cgctgtggcg ttaaacagaa ttttaaagct cattccgggg
     4921 attggctcag ggactgccgc ccgaattgtc gagtcagttg ttgaaaatgg tttcattgaa
     4981 gctgaaaccc acaaaaagaa aaaatattac gaagagataa agaagctgtt ttctcttatc
     5041 agctcaataa ctgaggaaaa acctgatgtg cttaaaactg tcagaattat tactgatttt
     5101 tatgcaccga tccttaaaac gatagaaagt gactttgaag aacggcttag agacattgat
     5161 gttcttatcc aaatcgcaaa aaattacaga tcgcttgaaa aattcctcac cgattttgca
     5221 cttgacccgc cgtcaactca gatcaatatg cttgctacac cagttgatgc agctcaaatc
     5281 agcaaaaaag tcatcctctc aacagttcac agcgctaaag gacttgaatg gaacacagtt
     5341 tttgtcgttc atctccttga cggatgcttc ccgtcagaaa gatcgctgaa aagaattgac
     5401 gaccttgaag aagaacgacg gctcttttat gtcgcctgct ccagagcaaa agagaaatta
     5461 tatctcactt tccccaacac cctttcattc tacggaggaa atttatctct tcccagcaga
     5521 tttatcgccg aaattgatga aaaattttat gagatt
//
LOCUS       DAOOYB010000190         5540 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_17007_length_5540_cov_8.389243, whole genome shotgun sequence.
ACCESSION   DAOOYB010000190 DAOOYB010000000
VERSION     DAOOYB010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5540
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17007_length_5540_cov_8.389243"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1223
                     /locus_tag="PL056_10290"
     CDS             <1..1223
                     /locus_tag="PL056_10290"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPM47444.1"
                     /translation="KMAFRNILKQKRRTFFTLFSMVVGFILLSISYGFNDGGYANIIK
                     AFTKARTGHIQIHKKGYLEKPGLYSNFVWDDKFIKTVSSIDSIESFTPRIHSGGLAFL
                     DKKTTAVQIKGIDPKREAETTLIDRKIESGSYLSASKDVRYEAVVTRSLADILNLKTG
                     DDLIVISSGADGSIANDRFKVIGIFSKDLDSLYSNAVFVPLNTTQEFLVLDGKIHEVA
                     VMINNYKNSFDDSMKISGIIKSAGIRNISVEPWEIVEEQFFKSMNADKEGTVLVVIII
                     MLVVGVGVLNTVLMSILERMREYGVMKAMGTTPSFIFFSIVIETFLMSLFASAAGLGL
                     SLLANWPFKVYGISYPEPVSIGGIFIETINSEYVAIAFYLPVIVIMVSSVVASIVPAY
                     KAASAEPVKSMRSY"
     gene            1233..2456
                     /locus_tag="PL056_10295"
     CDS             1233..2456
                     /locus_tag="PL056_10295"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPM47445.1"
                     /translation="MILKMAIRNIFRNKRRSLVAALAISLGLTALIFVDAFQEGMIVN
                     MVKSGTSTFMGEAQVHNSEFTNTMEVEKTINDPSKIMDELSKEELVSSLAPRIKTLGM
                     ITSAGNVQSVLITGIDPQKEKALSKMDEALIEGEYISDSSEKGIMIGQGLADILEVTV
                     GDRLVITLAQAHTGEMAQEMMRVKGIFKLGTREFDRSMAFIPIERARVMLGLEGAMHE
                     IAVNFKDHKKTWEKNLPIWTKYSNNGNKFENWMLLMPEMDAMLSMSAYSKYIVGFILF
                     LLVAGVIINTLFMSIYERMFEFGVMRAIGAKPVYTGTLVVFESAVLAVVSTILGIISA
                     LLLNLIFSKVGIDYTGTEFMSVAMTEFVYPVMTVEQYTSFPVSLIIFTMIVSIYPAWY
                     AAKIKPAVAMKKGTK"
     gene            2472..3155
                     /locus_tag="PL056_10300"
     CDS             2472..3155
                     /locus_tag="PL056_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012609738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPM47446.1"
                     /translation="MDVIKVENVTRTYKTGEIEVHALRGVDLTIRKGEFTAIAGPSGS
                     GKTTLLNIIGGLDTPSSGDVVLGGKLISKMSGSELSNFRRDHVGFIFQSYNLIPVLTV
                     EENIEFVMLLQGVERSLRSKKVKEILEVIGLTGSHDRLPSQLSGGQQQRVAVARAIVS
                     EPDIILADEPTANLDSVTGSALLDMMKKLNEEKGITFIFSTHDKMIMERAKRLVKLHD
                     GKIQSDETR"
     gene            3196..4368
                     /locus_tag="PL056_10305"
     CDS             3196..4368
                     /locus_tag="PL056_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47447.1"
                     /translation="MLQAEEGSFEIDLRGYSNTNLMFYRIPDGYQAFGIEKGTYFEFS
                     NITRAALISTPAEWVTVEVAYSIAPSVSDLNRRALAFNEPSVDRYRLIDVPELISEYE
                     YGSVSQNIDRVTAQFRTPFADITAGRQAISFGSGRFFNPTDVFSPFSYQSLNKEEKTG
                     VDALRVQVPIGKMSEIDLGYVIGEEGNWKKSAAFIRGKFDLKGTDLSIIAMEYKTHFM
                     TGINISSSVKGAGVWLEAAYTFSNLLEKYDNDNDFFRISAGADYYFSGDFYLFGEYHY
                     SSAGTIDKKDYLFNSRTKESAYKDGGVYLLGEHYFAPGFSWQVHPLVSLSLAVLMNLT
                     DPSAMISPKISFSLDENIMVDIGASINAGKRIKNSELESEFGAYPDFYYLSARLYF"
     gene            4461..>5540
                     /locus_tag="PL056_10310"
     CDS             4461..>5540
                     /locus_tag="PL056_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015734316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HPM47448.1"
                     /translation="MKDPRIVKLAKNLVRYSTSVKKGDNVLIEFKDHWPLPLVHALID
                     EIKKIGARAFVTYNDTQLQRKSLQHADDKWFETMAEIDLLRMKKMQAFIAINGRDNSY
                     ELADVPPENMKKYMEKYAKPVHFEQRVKHTNWCILAFPNPSYAQASSMSSEAFEDFYF
                     NVCTLDYAKMDKAMDPLKKLMDKTDKVHIKGPGRTDLKFSIKGLQGVKCAGKYNIPDG
                     EVFTAPVKDSVNGVIEYNTPTVYHGIKFDKVILTFKKGKIVKIEGSNVEKLEHIFNTD
                     EGARYVGEFAIGVNPYVTKPMVDILFDEKICGSIHFTPGATYDECDNGNRSAIHWDLV
                     MIQTPEYGGGEIYFDDKLIRKDGLFV"
BASE COUNT         1716 a         1031 c         1278 g         1515 t
ORIGIN      
        1 taaaaatggc attccggaac attctcaagc agaaaagaag gacatttttt acactttttt
       61 caatggttgt cggatttatc cttctttcta taagttacgg gttcaatgac ggcggttatg
      121 caaacataat taaagctttc acaaaagccc gtactggtca tattcagatc cacaagaaag
      181 ggtatctcga aaaaccggga ctttattcca attttgtgtg ggacgataag ttcataaaaa
      241 ccgtttcatc aatagattcg atagagtcat tcactccgag gatccattca ggcgggctgg
      301 catttcttga taaaaagacc acagcagtcc agataaaagg gatagatccg aaaagagagg
      361 ccgaaactac acttatagac agaaagatcg agtcgggcag ttatctttcc gcgtcaaaag
      421 atgtcagata tgaagctgtt gtcacccgtt ctcttgcaga tattctcaat ttaaaaacag
      481 gcgatgatct gattgtcata tcttcagggg ctgacggaag tattgcaaat gacaggttca
      541 aggtcatcgg gatattttca aaagatcttg attcgcttta cagcaatgca gtctttgttc
      601 cgcttaacac cacacaggaa tttcttgtgc ttgacgggaa aattcacgaa gttgccgtaa
      661 tgataaataa ctataagaat tcgttcgatg attccatgaa gatttcagga attatcaagt
      721 cggcaggaat ccggaatatc agtgtcgaac catgggaaat tgttgaagaa cagttcttta
      781 aaagtatgaa tgcagacaaa gaagggacag tacttgtcgt cataataatt atgcttgttg
      841 tcggggtcgg agttctaaac acagttttaa tgtcaatact tgaaaggatg agggaatacg
      901 gcgtaatgaa agcaatggga acaacaccgt cattcatttt cttttccatt gttatcgaga
      961 cttttctcat gtcacttttt gcaagcgccg caggacttgg cttaagtctg ttggcaaact
     1021 ggccgttcaa agtatatggc ataagttatc cggaacctgt ttcgataggg gggatattca
     1081 ttgaaactat aaattcagaa tatgtcgcca tagcgttcta tctgccggta atcgtaataa
     1141 tggtttcatc agttgttgca agcattgttc cggcatataa agcggcatcg gcagaacctg
     1201 taaaatcgat gcgttcatat taggagtggc aaatgatatt aaagatggca atacgcaata
     1261 ttttcagaaa taaaagaaga tcactggtcg ctgcactggc aataagtctg ggccttacag
     1321 cactgatatt tgtcgatgcc tttcaggaag ggatgatcgt gaatatggtt aaatccggca
     1381 cttccacatt tatgggcgag gcacaggtgc ataattctga attcaccaat acaatggaag
     1441 tggaaaagac cataaacgat ccttcaaaga tcatggacga actttcaaaa gaggagcttg
     1501 tcagcagtct ggcaccgagg atcaagactc tcggaatgat aacaagtgca ggaaatgtcc
     1561 agtctgtact catcacaggc atagatccgc aaaaagagaa agcgctctca aaaatggatg
     1621 aagcgttgat agaaggtgaa tatatttccg atagttccga aaagggaata atgatcggtc
     1681 aggggcttgc agatattctt gaagttacag taggtgacag actcgtcata actcttgctc
     1741 aggcacatac tggtgaaatg gcccaggaga tgatgagggt aaaagggata tttaagctcg
     1801 gaacaagaga gtttgacagg tccatggcgt tcattcctat cgaaagggca agagtaatgc
     1861 tcgggcttga gggagctatg cacgagatcg cagtgaattt taaagaccat aagaaaacat
     1921 gggagaaaaa tctgccgata tggacaaagt attcaaacaa tggaaataaa tttgagaact
     1981 ggatgctgct tatgcctgaa atggatgcaa tgctttcgat gtctgcatat tcaaaatata
     2041 ttgtcggttt tattcttttt ctgcttgtcg ccggagtaat aataaacaca ctttttatgt
     2101 caatttatga aaggatgttt gagttcggcg taatgagagc gataggagca aaacctgttt
     2161 ataccgggac tctcgttgtt tttgaatctg cagtgcttgc tgtcgtgtca acgattctgg
     2221 gaataatttc agcccttctg ctcaatctga tattttcaaa agttggaata gactataccg
     2281 gcactgaatt catgagtgtt gccatgaccg aatttgtcta tcctgtgatg acagtagaac
     2341 agtacacttc ttttcctgtc tctttgataa tatttacaat gatagtttca atatacccgg
     2401 cgtggtatgc ggcaaaaata aaaccggctg ttgcaatgaa aaaaggaaca aaataaattt
     2461 aggaggagat aatggatgtt ataaaagttg aaaatgtaac cagaacctat aaaaccggcg
     2521 agatagaggt tcacgcatta agaggggttg accttacgat aagaaaaggt gaattcacag
     2581 caattgcagg accttccggc tcaggtaaga ccaccttgct caatataatc ggcggacttg
     2641 acactccaag cagcggagat gttgtgctcg ggggaaaact tatcagcaaa atgagcggaa
     2701 gcgaactttc aaatttcaga agggaccatg taggattcat ttttcagagt tacaacctga
     2761 taccggtact tacagttgag gaaaacattg agttcgtgat gcttcttcag ggtgttgaaa
     2821 gatcactcag gtctaaaaaa gttaaggaaa tacttgaggt tatcggcttg actggcagcc
     2881 acgaccgtct cccttcccag ctttctggcg gtcagcagca gagggttgcg gtagcccgtg
     2941 ccattgtttc cgagccggac ataattcttg ccgatgagcc gacagcaaat cttgattcag
     3001 tcaccggctc agcacttctt gacatgatga agaaactcaa tgaggaaaag gggataactt
     3061 tcattttttc aacgcatgac aaaatgataa tggaacgggc aaagcggctt gtaaaacttc
     3121 atgacgggaa aattcaaagc gatgagacaa ggtaagatct taattttatt tgtttttatt
     3181 ttttcagtaa ttggtttgct gcaagctgag gaaggttcgt ttgagataga tctgagaggt
     3241 tactcaaaca ccaatctcat gttctaccgc attcctgacg ggtatcaggc attcggaatt
     3301 gaaaaaggga catattttga gttttccaac attacaaggg cggcactcat ttcaactcct
     3361 gcagaatggg tgaccgtgga agttgcttat tcgatcgctc cgtctgtatc agatcttaac
     3421 cggcgtgctc ttgcattcaa tgagccgagc gttgacagat accggctgat agatgttcct
     3481 gaattaatta gcgaatatga atacggcagt gtttctcaga atattgacag agtgactgca
     3541 cagtttcgga ccccgtttgc cgacattact gccggaagac aggcgatatc ttttggaagc
     3601 ggaaggtttt ttaatccgac agatgttttt tcaccatttt cgtatcagtc actcaacaaa
     3661 gaggaaaaga caggagttga tgctctgagg gttcaggttc cgattggaaa aatgagcgaa
     3721 attgatcttg gatatgtaat cggagaggaa ggaaactgga agaaaagtgc tgctttcatc
     3781 cgtggaaagt ttgatctgaa agggacagat ctttcaataa ttgcaatgga atacaagact
     3841 cattttatga cagggattaa cataagttca tcagtaaaag gggcaggggt gtggcttgag
     3901 gcggcatata ctttctcaaa ccttcttgaa aaatatgata atgataatga tttcttcagg
     3961 atctcagcag gtgcagatta ctatttttca ggcgatttct atcttttcgg ggaataccac
     4021 tattcttcgg cagggacaat tgacaaaaag gattatcttt ttaattcacg aacaaaagag
     4081 agcgcgtaca aagacggagg tgtctatctt ttaggtgagc actatttcgc acccggtttc
     4141 agctggcagg ttcatccgct tgtttcatta tcgttagcgg ttttaatgaa tcttacagac
     4201 ccttctgcca tgataagccc caagatctcg ttctcacttg atgaaaacat aatggttgac
     4261 atcggagcat caataaatgc aggaaaaagg ataaagaaca gcgaacttga aagcgagttt
     4321 ggagcatacc ccgattttta ctatctgagt gcacggctct atttctgata aacacctgtt
     4381 ccggccatta aaattgacat gacttttcac tgatataaaa tcagctgtta tcggttttat
     4441 tcacttttga agaggttaag atgaaagatc caagaattgt aaaactggca aagaatctgg
     4501 tgcgttacag tacaagcgtt aaaaaagggg acaatgttct cattgagttc aaagaccact
     4561 ggccgcttcc tcttgttcat gcgttgatcg atgagataaa gaagatcggt gcaagggcat
     4621 tcgtcactta caacgacaca cagcttcaga gaaagtcact tcagcatgct gatgacaaat
     4681 ggtttgaaac aatggctgaa atcgatctcc tgcgaatgaa gaaaatgcag gcgttcattg
     4741 cgataaacgg tcgcgataac agttatgagc ttgccgatgt tccgcctgaa aacatgaaga
     4801 aatatatgga aaaatatgca aaaccggttc actttgaaca gcgtgtaaag cacacgaact
     4861 ggtgtattct cgctttcccg aacccgagtt atgcgcaggc atcttcaatg agttctgaag
     4921 catttgaaga tttctatttc aatgtctgta cgctcgatta tgcaaagatg gataaggcaa
     4981 tggatccatt gaagaaactt atggataaaa ccgacaaggt tcatatcaaa ggtccgggca
     5041 gaacagacct caaattctcg attaaaggac ttcagggtgt caaatgtgcc ggcaaatata
     5101 acattcctga cggtgaagtg ttcacagctc ctgttaaaga cagcgtaaac ggagtgatcg
     5161 aatataacac accaactgtt tatcacggaa taaaattcga caaagtgatc cttaccttca
     5221 aaaaagggaa gatcgtaaag atagaaggga gcaatgtcga aaaacttgag cacattttca
     5281 acactgatga aggggcaagg tatgtcggtg agtttgcaat aggtgttaat ccttatgtca
     5341 caaagccgat ggttgacatc ctttttgacg aaaagatctg cggatcgatc cactttacac
     5401 ccggcgcgac ctacgatgaa tgtgataacg gaaacagatc ggcaattcac tgggaccttg
     5461 ttatgatcca gaccccggaa tacggcggcg gcgaaattta ttttgacgac aaacttataa
     5521 gaaaagacgg gttgttcgtt
//
LOCUS       DAOOYB010000191         5528 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_17063_length_5528_cov_10.886168, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000191 DAOOYB010000000
VERSION     DAOOYB010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5528
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17063_length_5528_cov_10.886168"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="PL056_10315"
     CDS             <1..492
                     /locus_tag="PL056_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47449.1"
                     /translation="DSANYGVAGEPEVLHYEATEDRTLFIVVEGTEGASGDYTLDVKE
                     WVEETDDDTIPDEDTVDEDIIDVDDTDDFDDVDTVDEEEDIDEIDDEDTGDTGNTGDT
                     GDSGDTGDSGDSGNSGDTGNTGDTGNTTVDEDETDSDSDELNDDDTIEKLGGSGCSCG
                     IVF"
     gene            complement(489..1364)
                     /locus_tag="PL056_10320"
     CDS             complement(489..1364)
                     /locus_tag="PL056_10320"
                     /inference="COORDINATES: protein motif:HMM:NF013954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HPM47450.1"
                     /translation="MHKKTVFLTVFLFLINSLDATVVLSDFDEINATHKLIDYTWKSQ
                     SVPRITVKKFPEKIKDVSNGKRLKFFIRTLLPMILMENETIEKEHDFIVKIAAKENWN
                     KNDISIISEIAYKYRIIKKNSDLTGLSEIEKDEIRYFLDHRIRPVPVPIALGQAALES
                     GWGTSRFVFEGNNLFGHVAKDHKKGLKPKNWSGKQRNIRIFDSLQDSISAYMLNLNRN
                     RAYRKFRWLRRKYPGNSSKIAEGFGNYAIIKEEYIGRLQQVITKYGLYRLNDSKLGNG
                     EKAIIHKKIKQIYIN"
     gene            complement(1379..1455)
                     /locus_tag="PL056_10325"
     tRNA            complement(1379..1455)
                     /locus_tag="PL056_10325"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Arg,seq:ccg)
     gene            complement(1468..1770)
                     /locus_tag="PL056_10330"
     CDS             complement(1468..1770)
                     /locus_tag="PL056_10330"
                     /inference="COORDINATES: protein motif:HMM:NF014534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HPM47451.1"
                     /translation="MNINYAFRGFEENSDGLRDYASKKLGKVDKLVSANTLIEVIFQK
                     DKNLKFTEIKLNHEGEDFIARYSDEKEFSRSIDLCVDKLTKQIKKAKDRKVDKRRD"
     gene            complement(1836..3263)
                     /gene="hflX"
                     /locus_tag="PL056_10335"
     CDS             complement(1836..3263)
                     /gene="hflX"
                     /locus_tag="PL056_10335"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HPM47452.1"
                     /translation="MYLSKELVLKIIREKPLNSTKLILMKNGAAVKTEIFRKNWDVFN
                     FIGDLRPDNTFDAIAVASMSKPVFSNVKTLMDGLLLKAFFIIDHTEEKIPQNIEVFFR
                     KGNKIKNLTSPFFLFNESIDVVLEKNRINAAAFFSSDDNPSSIAENIKLAEDAGYNVI
                     FSAVLKRPEMKKCKNQIAEHLKNIDENSISTILMFFKISAGEISAIEQFTGFEIISRD
                     DLIITIFDKRADGTSGKLKLADAVLAKEKAQFRKKVTGLSRIKGGIGLKGPGETKEEE
                     RKRILKNKEKDVRKKLKNEFSRISSQMKYRKKSNAKTVAIVGYTNAGKSTLFNHLTNE
                     TISLESDSFFSSIDPKIKRISLFGKPLFLVDTVGFISNMTKNITDAFLATLEEIATAD
                     MVLHIIDSSEKGWEKRKKFIEEILESNGTPENNIFTLFSKSDAITIKHPVRKGFFYNS
                     FNPLDITKIKKFIYSHIFGLDPFLE"
     gene            complement(3263..3700)
                     /locus_tag="PL056_10340"
     CDS             complement(3263..3700)
                     /locus_tag="PL056_10340"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HPM47453.1"
                     /translation="MTNRLSRIISETKSTFLTILLNWEKIAGKTNREIMIPFDLKNKV
                     LLVAVPNGMVAKTVVRFKEQMIGNINREIRKSAVSEIKFFVDSARFGNVEIAEPAQTE
                     RKIEISKTDLSRKIKELQKQGISEHLAGTLAEIELLIEKRKNS"
     gene            complement(3697..4545)
                     /locus_tag="PL056_10345"
     CDS             complement(3697..4545)
                     /locus_tag="PL056_10345"
                     /inference="COORDINATES: protein motif:HMM:NF014881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HPM47454.1"
                     /translation="MLLKGKELADRIVAKLQNENIPPLTLAVFHPVGDGAAESYLKMK
                     EKWLLKINAKIKVFPVSSSTPLVKFYEWIDEVNNDPSIHGIMVELPLPIEVNYWNLHT
                     KINPAKDVDGITFRNQGEFFATITEKIVPCTAVASIRLLEHFDIPIVGKHALVIGRSN
                     IVGLPLFKLFLNRNATPTIAHRHTYDLKTLIRTSDIIAIAAGKKGLVDSNDVKDGATV
                     IDIGINVADDGSVSGDFNVANDTEKERINYSPVPGGVGVVTNAVMLENLAKCYKLQNN
                     LSRDQF"
     gene            4657..4899
                     /locus_tag="PL056_10350"
     CDS             4657..4899
                     /locus_tag="PL056_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47455.1"
                     /translation="MNTFRFFHLYLLAGRIAFAVSGRIGVSPLAIKFNLRGKIFEWDP
                     LIKKKFGNIIPLKPLDFDLFSIGRGIKVMGNEVATF"
     gene            4984..5367
                     /locus_tag="PL056_10355"
     CDS             4984..5367
                     /locus_tag="PL056_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47456.1"
                     /translation="MRTLILALTVMMMLLSCVEDEDINGNGDGKGWEKEWSSEARDRL
                     YAIAVSPDDTIYVGGMTLGNLYSEKEGEYDATLAAFNPKSDKLWGRQWSVNDDENEVW
                     GLVVDDEGYIYVGGGVESFCNEILS"
BASE COUNT         1524 a         1031 c         1146 g         1827 t
ORIGIN      
        1 gattccgcaa attacggagt tgcgggggaa ccggaagttc ttcattatga agcgacagaa
       61 gacagaactc tcttcatagt tgttgaagga acggaaggtg catcaggtga ttacacactt
      121 gatgttaaag agtgggttga agagacagat gacgacacaa ttcctgacga agatactgta
      181 gatgaagaca ttattgatgt tgacgataca gatgatttcg atgatgttga cacagttgat
      241 gaagaagaag atattgacga aatagacgat gaagacaccg gagacacagg caacactgga
      301 gacacaggtg attccggaga tactggagat tcaggagata gcggaaactc cggcgacact
      361 ggtaatactg gagacactgg caacaccaca gtagatgaag atgaaactga ctcagatagc
      421 gatgagctta atgatgatga taccatcgaa aaattaggcg gctccggctg cagttgcgga
      481 atagtattct aatttatata tatctgcttg attttcttgt gaattatcgc tttttcacca
      541 ttgccgagct ttgaatcatt cagacggtat aaaccgtatt ttgttatgac ctgctgcaat
      601 ctgccgatgt attcttcttt tattattgca tagttcccga atccttcagc tatctttgac
      661 gagttgccgg gatattttct tctgagccat ctgaattttc tgtaagccct gtttctgttg
      721 aggtttagca tataagccga aattgagtcc tgaagagaat caaatattct tatgtttctc
      781 tgtttcccgc tccagttttt aggtttcaac ccttttttgt ggtcttttgc aacatggccg
      841 aaaaggttgt tcccctcaaa tacgaatctg ctggttcccc agcccgattc aagtgctgcc
      901 tgtccgagag cgataggaac aggcacaggt cttattctgt gatcaaggaa ataccgtatc
      961 tcatctttct caatttcaga caaaccagtc aagtcgctat ttttcttgat aattctgtat
     1021 ttatatgcaa tttcggaaat gatgctgatg tcatttttat tccaattttc ttttgccgca
     1081 attttgacaa tgaaatcatg ctccttttca atcgtttcat tttccataag aatcatcggg
     1141 aggagggttc tgatgaagaa tttcaatcgt ttcccattgc ttacatcttt aattttttca
     1201 gggaatttct ttacagttat tctcggaact gattgcgatt tccaagtata atcaatgagt
     1261 ttgtgtgttg cattgatctc atcgaaatca ctgagcacaa ctgtggcgtc aagagaattt
     1321 attagaaata gaaatactgt aagaaaaacc gtttttttat gcattcaaat ttcaaaagtg
     1381 gtaggcgaga cgggattcga acccacgacc tttggctccg gaggccaacg ctctatccaa
     1441 ctgagctacc cgcccacaat aaagcagtta gtcccgcctt ttgtcaacct tgcggtcttt
     1501 tgctttcttt atctgttttg taagcttgtc aacacaaagg tctattgatc tagagaactc
     1561 cttttcatct gaatatctgg caatgaaatc ctcgccttca tgatttagtt tgatctctgt
     1621 gaatttgaga tttttgtctt tctgaaaaat aacctcgatc aaagtgtttg ctgaaacaag
     1681 tttgtcaact ttaccaagtt tttttgaagc gtaatccctt agtccgtcac tgttttcctc
     1741 gaatcctctg aaagcataat taatgttcat atcaacctcc tcaggtaata atgactttta
     1801 acactaccac atatgtgaaa aagtgtcaac agatgttatt ctaaaaaagg gtctaagccg
     1861 aaaatgtgac tgtatataaa cttttttatt tttgtaatgt ctaaaggatt gaatgagtta
     1921 tagaaaaatc cttttctgac aggatgtttg atcgttatgg catcactttt tgaaaaaagg
     1981 gtgaaaatgt tgttttcagg tgtcccgttg gattcaagga tctcttcaat gaactttttt
     2041 cttttttccc atcctttttc acttgaatct attatatgca gtaccatatc agcagtagca
     2101 atttcctcaa gtgtggcgag aaaagcatca gtaatatttt tagtcatgtt tgatatgaat
     2161 ccgactgtgt caacaagaaa aaggggtttc ccgaaaagag atattctttt tattttaggg
     2221 tcgatcgaac tgaaaaaact gtcactttcc aacgaaatgg tttcgtttgt caagtggttg
     2281 aaaagagttg attttccggc atttgtatat ccgacgatgg caactgtctt tgcattgctc
     2341 tttttgcggt atttcatctg ggaggatatc cttgaaaatt catttttcag tttttttctt
     2401 acatcttttt ctttgttttt cagtatcctt tttctttcct cttctttcgt ttctcctgga
     2461 cctttaagtc ctatgccgcc tttgatcctg ctaagtccgg tgaccttttt tctaaactgc
     2521 gccttttctt ttgcaagtac ggcatctgca agtttcagtt ttccgctggt tccgtcagcc
     2581 cttttatcaa atattgtgat tatcagatca tctctggaaa ttatttcaaa acctgtaaac
     2641 tgttcaatgg ccgatatttc accggctgag atcttaaaaa acataagaat tgttgagatt
     2701 gaattttcat caatgttctt aagatgttca gcgatctgat tcttgcattt tttcatttca
     2761 ggtcttttta aaacagcaga aaaaatgaca ttatatcctg catcttcggc aagttttata
     2821 ttctcagcga tcgatgaagg gttgtcgtca gatgaaaaaa aagcggcggc attaattctg
     2881 tttttttcta aaacaacatc aatgctctcg ttgaaaagaa agaaagggga tgtcaaattc
     2941 tttattttat tccctttcct gaaaaaaacc tcgatattct gaggtatttt ctcttcagtg
     3001 tgatctataa taaaaaaagc ttttaaaagg agtccgtcca ttaaagtctt tacatttgag
     3061 aacaccggct ttgacattga tgcgacagcg attgcgtcaa aggtgttgtc tggccgaaga
     3121 tctccaataa aattaaacac atcccagttt ttacggaata tttctgtttt gactgccgct
     3181 ccatttttca tcagaatgag ctttgttgaa tttaacggtt tttcccggat gatcttaagg
     3241 acaagctcct ttgaaagata cattaactgt ttttccgctt ttctatgaga agttcaatct
     3301 cggcaagcgt tcctgcaagg tgttcgctga tcccctgttt ctggagttcc ttaattttcc
     3361 tggagaggtc ggtttttgaa atttcaattt ttctttctgt ttgagcaggt tctgcaattt
     3421 ccacatttcc gaatcttgca ctgtctacaa agaattttat ttcagaaacg gcactttttc
     3481 ttatttctct atttatgttg ccgatcatct gctccttgaa ccggacgact gtttttgcaa
     3541 ccattccgtt gggaactgcg accaaaagga ctttgttttt taagtcaaaa gggatcatta
     3601 tttcgcggtt tgtctttcct gctatctttt cccagtttaa aagaatggtc aaaaaagtgc
     3661 ttttcgtttc tgaaatgatc cttgaaagtc tgttcgtcaa aactgatccc ttgatagatt
     3721 gttctgtaat ttatagcatt tggcaaggtt ttcaagcatg acggcatttg taactactcc
     3781 aactccgcca gggactggtg aatagtttat ccgctctttt tcagtgtcgt ttgccacatt
     3841 gaagtcacct gatacggaac cgtcatcagc aacatttatt ccgatatcaa tgacagttgc
     3901 gccgtctttg acatcattgg aatcaacgag cccttttttc cctgcggcaa ttgcaataat
     3961 gtctgaagtc cgaatgagag ttttcaggtc ataggtgtgt ctgtgggcga ttgtcggggt
     4021 tgcatttctg tttagaaaaa gtttgaaaag cggcagaccg acgatattac ttcttccaat
     4081 gacgagggca tgtttcccga cgataggaat gtcaaaatgt tcaaggagcc ggatcgatgc
     4141 aacggcagtg catggaacga tcttttcggt gatcgttgca aaaaattctc cctgattacg
     4201 gaatgttatc ccgtcaacat cttttgccgg atttatcttt gtatgaagat tccagtaatt
     4261 cacttcgatc ggcagaggaa gctcgaccat aatcccgtga attgaaggat cgttgtttac
     4321 ttcatcgatc cactcgtaaa attttacaag cggtgtgctt gatgaaactg gaaacacctt
     4381 gatctttgca ttgatcttta agagccattt ttccttcatt ttgagataac tttcagccgc
     4441 accgtcaccg accggatgaa aaacggcaag tgtaagaggc ggaatattct cattttggag
     4501 ttttgcaaca attctgtcgg caagttcttt tccttttaaa agcattttct ttcctcataa
     4561 aaacaaaaac attactgatt atatgaaaga gacgactgat gtcaaaaaga ggtgtttgat
     4621 tttctcataa agattcctgc atctgagatg gagtgaatga atacattcag gttttttcac
     4681 ctgtatcttc ttgccggaag aattgccttt gcagtgagcg gcaggatagg agtatctcct
     4741 cttgcgatca agttcaatct gcgagggaag atatttgagt gggatccgtt gataaagaag
     4801 aaattcggca acataatccc gctcaaacca ttggattttg atctgttctc aattggtcgc
     4861 ggaataaaag taatgggtaa tgaagtggca acattctgac aagtggaaaa ggatagatct
     4921 ataatttgaa aaatcacaaa tttatgatag cattatcatg ctgaacacgg aggagggaca
     4981 ttcatgagaa cattaatttt ggcactaaca gttatgatga tgttgttatc atgtgtagaa
     5041 gatgaagaca ttaacggcaa cggtgatggg aaaggatggg agaaggaatg gagttctgaa
     5101 gcaagggaca gactttacgc aattgctgta agtcccgatg acacgattta tgtcggtgga
     5161 atgacgctgg ggaatttgta ttcagaaaaa gaaggggaat atgacgcaac tcttgcagca
     5221 tttaacccaa aaagtgataa gctgtggggc agacagtggt ctgtcaatga tgatgaaaac
     5281 gaggtttggg gactagttgt ggatgatgag ggctatatat atgtaggggg gggggtggaa
     5341 tccttttgta atgaaattct ctcctgacgg gtctaagatc tgggaaaagt ttcctgaagt
     5401 tgtcagcgtt ctttgtttag cacttgataa agccgggaat gtttatgcag gcacaggcta
     5461 tggcgacatc ctgaaattct cccctgaagg aaaagagttg tggcatcata atatttcaac
     5521 agaagatg
//
LOCUS       DAOOYB010000192         5502 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_17161_length_5502_cov_9.114742, whole genome shotgun sequence.
ACCESSION   DAOOYB010000192 DAOOYB010000000
VERSION     DAOOYB010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5502
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17161_length_5502_cov_9.114742"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(200..1285)
                     /locus_tag="PL056_10360"
     CDS             complement(200..1285)
                     /locus_tag="PL056_10360"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde reductase"
                     /protein_id="HPM47457.1"
                     /translation="MDNSLFYDISVLPSLIYSEKTLFITMPDESLENADVSGAVRKII
                     SEKSAVHLTYSGSALPVEDVQQLYDNIKEKDFSFIVAVGGGTVMDIAKIVALALSNKL
                     ERIDAILADPSGFQNVKKLIFVPTTCGTGSEATHFAVVYKTGKKYSVAHHTIRAEAVI
                     LDPSFLINLPEKIRNATVLDALSQAVESMWAKGATEESSGYAKEAVGLILKGIQSLDN
                     IAKLKYFQKGSYLAGKAINISKTTAAHAISYPLTAKFKIPHGVAVFLTLPYLAEMNFN
                     DKTLPVFDELFKLFNVRNINDLSNSLKNIMNSMGFITDIYDYGVTENDFESIAGQSIV
                     PGRSDNNPVDIDIAAVIDILKKTTFKN"
     gene            complement(1278..2405)
                     /gene="aepY"
                     /locus_tag="PL056_10365"
     CDS             complement(1278..2405)
                     /gene="aepY"
                     /locus_tag="PL056_10365"
                     /EC_number="4.1.1.82"
                     /inference="COORDINATES: protein motif:HMM:TIGR03297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033980 - phosphonopyruvate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonopyruvate decarboxylase"
                     /protein_id="HPM47458.1"
                     /translation="MIDPEIFVTYLAENGTDLFTGVPDSTFKGFTTFINSGGPGFKHL
                     AASNECEAVGIASGYHLATGKIPVVYMQNSGFGKTVNPYTSLASKDVYSIPMLMMIGW
                     RGEPGKKDEPQHKMMGRITKDLLNVLEIENCELDMENWQEQIISALKYCSQNSCPFAI
                     IVKSGMFSEPCVEKKKSDNPLPLREEILEKIVAAAPENALFISTTGKTSRELFEIREK
                     FNLPHDTDFYTVGSMGCASSIALGVATGDHGRPVFIIDGDGAALMQTGTFAALGKHGQ
                     NRNITHIIIDNQAHESTGGQPTLSSTVSFEGVAKNCGYKFVYSASSIEEVEKTIPELV
                     KNNELKLLVVRSKKGSRPDLGRPTTTPVENKTNFMNCIKNG"
     gene            complement(2405..>3082)
                     /gene="aepX"
                     /locus_tag="PL056_10370"
     CDS             complement(2405..>3082)
                     /gene="aepX"
                     /locus_tag="PL056_10370"
                     /EC_number="5.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013658536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050188 - phosphoenolpyruvate mutase
                     activity [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate mutase"
                     /protein_id="HPM47459.1"
                     /translation="VDLTSRMNTVSDILEVTTKPIIFDGDSGGLTEHFVFMVRTLERE
                     GVSAVIIEDKVGLKKNSLFGTEVEQNQDSIENFCEKIRTGKKAQVTQNFMIIARIESL
                     ILSAGMDDAIKRAEAYIAAGADGIMIHSKEKSPDEILEFCRRYSEFNVKVPLVVVPST
                     FNSITETELKNAGVRIVIYANQLLRSAYPAMIKTAETILENERAYEADKACMPIKEIL
                     TLIPERT"
     assembly_gap    3083..3182
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3183..3503)
                     /locus_tag="PL056_10375"
     CDS             complement(<3183..3503)
                     /locus_tag="PL056_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007930206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HPM47460.1"
                     /translation="MNKKVYVAMSADLIHPGHLNIIEEARKYGDVLIGLLTDRAIASY
                     KRLPYMTFDQRKKVIENIKGVAEVVPQETLDYVPNLEKYKPDFVVHGDDWKTGVQKEV
                     RQKVI"
     gene            complement(3516..4106)
                     /locus_tag="PL056_10380"
     CDS             complement(3516..4106)
                     /locus_tag="PL056_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47461.1"
                     /translation="MKNLFLLTFFLVQLLTLDLYSKQSVQSIMLHNDGYKMFQEFRPN
                     DTILKNTIREMDELARKNKNKVFSESASLLRTIQNKCKYYYDTNTALIEHFKKKWDQP
                     HYAKMEDEWKQTTFDRDRKKVKDVYSLTQGMLLAFNDWNTARNSAVKKPTNEEIVKGI
                     QAANEILKKTIDAVLADESAIFEAISKELQIIKLSK"
     gene            complement(4127..>5502)
                     /locus_tag="PL056_10385"
     CDS             complement(4127..>5502)
                     /locus_tag="PL056_10385"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HPM47462.1"
                     /translation="HFRRFLSSFLLKIERFRRKNVLVILLDRFIGGYIPELTELIPEL
                     KSEILDGFVWYPRSLSSGSDTLSSEPSIMGGWDYHAETMNTSRQNVPLLDRMNESIRV
                     LPYNFTRAGFNSNIYGYLSSWLKADDKTHLENTAVENLTGKYLDTWLKEKELDAERSD
                     FIEKLAVFGFFRVSPPFLRTAVYDDGHWNLEGSKPDFVEKKKGKAKNEKISFEEPGYV
                     SWKETNKSLIANYLKNYSTLDYLTRISSASETDKGQFYYFSSKLTHEPWLTGSDFVIS
                     NDRILKYPRELYKKYNKSMNALKHIYTDGAALKLAGEWFRWMKENGVYDNTRIIIVSD
                     HGRNIFNPMFSSQKINGSRTTPATWHNTVLVKDFNSRGEMTRNESFITTCDIPYIVLK
                     DIVEGENPYTGNRITEKKDKIPFTVMKTKFRIREQGKFKFTFTELFTVHDEDIFNLSN
                     WEKIDNL"
BASE COUNT         1457 a         1124 c         1021 g         1800 t
ORIGIN      
        1 tgtcaccagt attgccggtg tctccagtat ttccagtgtc acctgaatca cctactgcaa
       61 cccttttggt ctgattagtg tcacatgaaa taaaaagaaa aaaggataag atcaaaattg
      121 caaaaaatct gattttcata tcggcctccg gaatgaatcg cctctaaaaa acaaacgctt
      181 atctatttta tgacaaaaat taatttttga aagttgtttt ttttaagatg tctatgacag
      241 cagcaatatc aatatcaacc ggattattgt cagatctgcc gggaactatg gattgtcctg
      301 cgatagattc aaaatcgttt tcagtaacac cgtaatcata aatgtcggtg atgaatccca
      361 ttgaattcat tatgttttta agtgaattgc tcagatcatt gatgtttctg acattaaaca
      421 atttgaaaag ttcgtcaaaa accggaagtg tcttatcgtt gaaattcatt tctgcaagat
      481 atggaagtgt cagaaaaaca gcgacgccgt gaggaatctt aaatttagct gtaagcggat
      541 atgaaatggc gtgtgccgca gtggtttttg agatatttat tgctttacct gcaagatagc
      601 ttcctttttg aaaatattta agtttcgcaa tgttatcaag agattgaatc ccttttagga
      661 tcagtcccac cgcctcttta gcataaccgg atgattcttc cgttgctcct tttgcccaca
      721 tggattcaac tgcctgtgaa agggcatcaa gaacggttgc gtttctaatt ttctcaggaa
      781 gatttataag aaacgaagga tcaagtatga ccgcttcagc cctgatggta tgatgtgcga
      841 cagaatattt ctttccggtt ttatatacaa ccgcaaagtg tgttgcttcg cttcctgttc
      901 cgcaggtggt agggacaaat atcagttttt tcacattctg aaaaccggat ggatctgcaa
      961 ggatggcatc tattctttca agtttgttcg aaagagccag cgcaactatc tttgcaatgt
     1021 ccatgacagt tccgccaccg actgccacga taaaggaaaa atctttttct ttaatattat
     1081 cataaagttg ctgaacatct tcaactggca gagcagaacc tgaataagtc aaatgaacag
     1141 cgcttttttc tgatattatt ttgcgcaccg cacccgacac atcagcattt tcaagcgatt
     1201 catcaggcat tgttataaaa agggtttttt ctgaataaat aagtgacgga agaaccgaaa
     1261 tatcataaaa aagagagtta tccattttta atgcagttca tgaaatttgt tttgttttca
     1321 acaggtgtcg ttgtcggtct tccaaggtcg ggacgggacc ctttttttga tcttactaca
     1381 agaagtttca gttcattgtt tttaaccagt tcaggaatgg tcttttctac ttcttcgatt
     1441 gatgaagccg aatagacgaa tttataaccg cagttttttg caacaccttc aaaagaaact
     1501 gttgaagata aagtgggctg acccccggtt gattcatgtg cctgattgtc aatgatgata
     1561 tgagtgatat tcctgttctg tccgtgcttt ccaagagccg caaaagttcc cgtctgcatc
     1621 aaagcggcac cgtcaccgtc aattatgaaa accggtcttc cgtgatctcc tgttgcaaca
     1681 ccaagagcta ttgaagatgc acatcccatt gatccgacgg tataaaaatc ggtgtcgtga
     1741 gggagattga atttttctct tatttcaaaa agctctctgc ttgtctttcc tgtcgttgaa
     1801 atgaaaaggg cattttcagg ggccgccgca acgatctttt caagtatttc ctctcttaag
     1861 ggaagcggat tgtcagattt tttcttttct acgcatggct ctgaaaacat tccgcttttt
     1921 acgattatcg caaacgggca actgttctga ctgcagtatt ttaaagcact gattatctgt
     1981 tcctgccagt tctccatgtc aagttcacag ttctctatct caagaacatt gagaaggtct
     2041 tttgtaatgc ggcccatcat tttatgctgg ggttcatcct tctttcccgg ctcgccgcgc
     2101 caaccgatca tcattagcat cggaatggaa tagacatctt ttgaagcaag cgatgtatat
     2161 ggatttacag ttttgccgaa accggagttc tgcatgtaga caacaggtat cttcccggtt
     2221 gcaagatgat acccgctcgc aatgcctacc gcttcacatt catttgatgc ggcaagatgt
     2281 ttaaatccgg gaccgcccga attgataaat gttgtaaatc ctttgaaagt tgaatcagga
     2341 acccctgtaa aaagatcagt tccgttttcg gcaaggtatg ttacaaagat ctccgggtct
     2401 atcattatgt tctctccggg atgagtgtta atatctcttt tatcggcata caggccttgt
     2461 cagcttcata agccctttca ttttcgagga tcgtttcggc tgtttttatc attgcgggat
     2521 atgcacttct taaaagctgg tttgcatata taactattct gacccctgca tttttaagtt
     2581 cagtttctgt gatcgaattg aatgtggaag ggacaacaac aagcggaact ttcacattga
     2641 actcactgta tcttctgcag aattcaagga tctcatcagg agatttctct ttgctgtgga
     2701 tcattattcc atcggctccg gcggcaatat aagcttctgc cctttttatc gcatcatcca
     2761 ttcctgcact gagaatgaga gactctatcc ttgcgattat catgaaattc tgggtcacct
     2821 gcgctttttt acctgttctt attttttcac agaaattttc aattgaatcc tgattctgtt
     2881 ccacttctgt tccgaaaagt gagtttttct tcaatcccac cttgtcttct attatcactg
     2941 cagaaacccc ttccctttca agagttctca ccatgaaaac aaaatgttcg gtaagcccgc
     3001 cggaatcacc gtcgaaaatg atcggttttg tcgtcacttc gagaatgtct gaaacagtat
     3061 tcatccttga agttagatca acnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nntattactt tctgacgcac ttctttctgt acaccggttt tccagtcatc accgtgaacg
     3241 acaaaatcgg gtttgtattt ttcaaggtta ggaacatagt caagtgtctc ctgcggaaca
     3301 acttctgcca cacccttgat attctctatg actttttttc tctggtcaaa agtcatgtaa
     3361 ggaagccgtt tgtaacttgc gatcgccctg tcggtcagaa gaccgatcag aacatcaccg
     3421 tattttctcg cttcttcaat aatgttgaga tgtccaggat ggataaggtc ggcactcatt
     3481 gccacatata cttttttgtt catttctatc tcccgttatt tcgacaattt tattatctgc
     3541 aattcctttg aaatggcttc aaaaatggcg gattcatctg ccagaacggc atcaatggtt
     3601 tttttcagaa tttcatttgc cgcttgtatc ccttttacga tctcttcatt tgtcggcttt
     3661 ttaacagcgc tgttccttgc tgtattccag tcattgaagg caagaagcat cccctgtgtc
     3721 aaagaataga catctttcac tttttttctg tcacggtcga aagttgtctg cttccattca
     3781 tcttccattt ttgcataatg cggctggtcc catttttttt tgaagtgttc tataagtgcg
     3841 gtgtttgtat cgtaataata tttacattta ttctgaattg ttcttaaaag tgatgcgctt
     3901 tcggaaaaga ccttgttttt atttttcctt gccagttcat ccatttcgcg aattgtgttt
     3961 ttaaggattg tatcattagg cctgaactcc tgaaacattt tatatccgtc gttatgaagc
     4021 atgatcgact gaactgattg tttggagtaa agatctaaag tcagcaactg aacaagaaaa
     4081 aaagtcaaaa gaaacagatt tttcatggaa accccttaaa aaaacatcat aagttatcaa
     4141 ttttttccca gtttgacaaa ttaaaaatat cttcatcatg cactgtgaaa agctccgtaa
     4201 aagtgaattt gaatttgccc tgttctctta tccggaactt ggttttcata acagtgaaag
     4261 gtattttgtc ctttttttca gttatccggt ttccggtgta aggattctct ccttcaacaa
     4321 tatccttcaa tacaatataa ggaatgtcac aggttgttat aaatgattca ttccgggtca
     4381 tttcccctct tgaattaaaa tcttttacta aaaccgtgtt gtgccatgtt gcgggagtgg
     4441 ttcttgatcc attaattttc tgactgctga acatcggatt gaatatgttt ctgccgtgat
     4501 ctgaaactat tatgatcctg gtattgtcgt aaaccccgtt ttccttcatc cacctgaacc
     4561 attctcctgc aagtttaagc gccgctccat cagtatagat atgtttcagc gcattcatgc
     4621 ttttattata ttttttataa agttccctcg ggtattttaa aattctgtca ttgctgatga
     4681 cgaaatcact accggtgagc cacggttcat gggtaagttt ggatgaaaag tagtaaaact
     4741 gacctttgtc agtttcggag gcgcttgaaa ttctcgtcag atagtcaagt gtactgtaat
     4801 ttttcaagta attggcaatc agacttttat ttgtctcttt ccatgaaaca tagcccggct
     4861 cttcaaatga gatcttttca ttttttgcct ttcctttttt cttttctaca aagtcaggtt
     4921 tgcttccttc aaggttccag tgaccgtcat catatacagc ggttctcaga aaaggcggag
     4981 acactctgaa aaaaccgaaa acggccagtt tttcaatgaa atcagatctt tccgcatcca
     5041 gttctttttc tttaagccat gtgtcaagat attttccggt caaattctca acagcggtat
     5101 tttcaagatg ggttttatca tcagctttca gccagcttga aagataaccg taaatatttg
     5161 aattaaatcc ggcacgggtg aaattatagg gaagcactct gatcgattcg ttcatcctgt
     5221 caagaagagg aacattctgc cttgaagtat tcatcgtctc tgcgtgataa tcccagccac
     5281 ccattattga aggttcggaa gagagtgtgt cgctgcctga tgaaagcgag cggggatacc
     5341 agacgaatcc gtcaagtatc tcacttttaa gttcaggaat aagttcagta agttcaggaa
     5401 tatatcctcc gatgaatctg tcaagcagaa taacaagaac attctttctc ctaaatcgtt
     5461 cgattttcaa taaaaaactt gacaaaaacc tcctaaagtg aa
//
LOCUS       DAOOYB010000193         5396 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_17614_length_5396_cov_7.800037, whole genome shotgun sequence.
ACCESSION   DAOOYB010000193 DAOOYB010000000
VERSION     DAOOYB010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5396
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17614_length_5396_cov_7.800037"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="PL056_10390"
     CDS             complement(<1..403)
                     /locus_tag="PL056_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47463.1"
                     /translation="MDIFKSIKNDPGLLESVRYVFENTEINNNSLKNLIKYLREISAR
                     ENKQYSEILSIIKADEILKNPEMTGKTRSEEILRKLYHLRFPLWSSKQQEFKSIANRF
                     RALTGGEIIFPEFAEGNSFKISFEIRKQEDVD"
     gene            complement(501..2054)
                     /locus_tag="PL056_10395"
     CDS             complement(501..2054)
                     /locus_tag="PL056_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012676340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HPM47464.1"
                     /translation="MSSPKREQWGTKLGVILAVAGSAVGLGNFLRFPTQAALGGGGAF
                     MIPYFVSFLLLGIPLMWVEWSLGRKGGEYGHGTAPAILYKITGSRFIKYFASIGIIIP
                     LLILFYYAFIESWSLAYAFFSISGTLADVAGKNELAQFLQGYQGVVSNGYFDSIWIAY
                     GFFVVTIIINFYFIYKGVASGIEKLSKIAMPLLVIIAVLLVIRILTLGTPDPLKPDQS
                     VSNALNFVWTPDFTVLLNSKVWLSAAGQIFFTLSLGMGVIMTYASYLKKNDDIALSGL
                     TASATNEFCEVILGGSLIIPAAFIFFGSAETVRIATTGAFNIGFVTMPNIFANMPGGP
                     FFAFLWFMLLFIAGITSSVSMIQPAVAFLEDEFGFSRKKTVMIMFIFSFLACQPVIFF
                     MKYGFLDELDFWGGTVFLTIFATLEIVVFGWIYGIDKGWKDLNEGAEIKIPSIFRFII
                     KYVSPLFLFILLGVWTYQMFIPTIMMKGVPDENKPYIWGARIFILLIIGIMMYLIKIT
                     TGRKEEVKQ"
     gene            complement(2085..3233)
                     /locus_tag="PL056_10400"
     CDS             complement(2085..3233)
                     /locus_tag="PL056_10400"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPM47465.1"
                     /translation="MKTYDFLIIGAGSIGTPLAYHLAKKGKSVCVLEKHASTGRGQNR
                     AAIGGIRATHSDPAKIKICTMSLEEIRNMHSDHGIDIDWIEGGYLFPVYDTQRERNLK
                     DLLKVQKKFGLNIDWIDADALKALVPGITESGLRGGTFSPNDGSASPLKTTGAYFKLA
                     TKAGAEFRFYENVLSMEVKDKKVVSVKTNKETYSAGLIINAAGGDAKEIGKMYGVDLP
                     VYPDSHEAGITEPVKPFFKPMVVDIRPDSHSHNYYFYQNIEGQVVFCITPYPKAAGKD
                     TDNTSEFLPLMIKRMLELYPRLRNLRVRRTWRGLYPMTPDGFPIVGMAHEIENVFHAV
                     GMCGQGFMLGPGLGRILSEIFIDNSTEYNFILDQLTLYRDFSGEEMLK"
     gene            complement(3235..5304)
                     /locus_tag="PL056_10405"
     CDS             complement(3235..5304)
                     /locus_tag="PL056_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPM47466.1"
                     /translation="MNRIKVHPVLNVPKERHEVSFMFDGTQMKGFEDEMVSSALFANG
                     VKQFSIHRKGDAPQGIFCANGQCSQCTVIIDGRPLKSCITPLKEGMDVRTMKHLPDLP
                     ADDSPMAGYERKCVVCDVLVIGGGPSGLTSTIELAKLGFRVVLVDDKDKLGGKLLLQT
                     HKFFGSEEDCYAGTRGIDIANLLEEEVRKYSNVKIFTETSIVGVYKDKKAGLFVENRH
                     YVLVDFKGVLITAGAREKSIVFPGNDLPGVYGAGAFQTLVNRDLVKASDKVLIIGSGN
                     VGLIGAYHALQAGIEVAGIVEIAHEISGYKVHADKIKRMGVPLYLNTTVLCAEGKGKV
                     ERVTIAEVDSAWNPVLETARTFEVDTLLVAAGLTPTDEFFDMAEKFEFNVIKAGDAEE
                     IAEASSAMFGGRIAGLKMAALLGRKDVVVDDTWYKKAEVLKSRPGIVHPLNPVKMAEK
                     FQPNFSCLQEIPCNPCTTVCPKNAIKLMDKSGTIMDIPYLAGECIGCGICVAACPGLA
                     ITLVKKVDEKTAEVMLPHEFNCDFVVGDMIPLTDMDGSYLEDGKITKITFNKKYKTAL
                     ITVAVSLNNAAKVMGIRVQDESVTRPLKTADYEYIPENGIVCRCERVTLKEVVDFIKE
                     NKIRDINQLKQIRVGMGACGSKTCSVQMPRVFKAAGVNWSEVTEGTNRPMCVETPMYA
                     LINEDGE"
BASE COUNT         1482 a         1304 c         1031 g         1579 t
ORIGIN      
        1 tatcgacatc ttcctgtttc ctgatctcaa aagagatctt aaagctgttc ccttcggcaa
       61 attcggggaa tattatctct ccgcctgtca gcgccctgaa cctgtttgca atgctcttga
      121 actcctgctg ttttgagctc cagaggggaa atctgaggtg atataatttt ctcaatatct
      181 cttcacttct ggtttttcct gtcatttcag ggttttttaa tatctcgtcc gcttttataa
      241 tggataatat ctctgaatac tgcttatttt cgcgggccga gatctctctt aaatatttta
      301 tcagattctt aagagaattg ttgtttattt cagtattttc aaatacatat cttaccgact
      361 caagaagtcc gggatcgttt tttattgatt taaatatgtc catgttcatg ctttttttga
      421 aaaaacttta cagaaacaga aagtcaaaag ggacaagatc gcactccata caagaaccat
      481 cagaattatt gcacctgtac tcattgcttc acctcctctt ttcttccggt tgtgatcttt
      541 atcaggtaca tcattattcc tattataaga agaataaaga tgcgggctcc ccatatgtag
      601 ggcttattct catccggaac tcctttcatc attatcgtgg gaatgaacat ctggtatgtc
      661 cagacaccca gcagaatgaa aaggaaaagc ggagagacat attttattat gaatctaaat
      721 attgaaggga tctttatttc agcaccttcg ttcagatctt tccacccttt gtcaatgccg
      781 tatatccaac cgaaaacgac aatttcaagt gtcgcgaata tcgtcagaaa aacagttcct
      841 ccccagaaat caagttcatc aagaaaaccg tatttcatga agaatattac cggctgacat
      901 gcaagaaacg agaaaatgaa cattatcata acagtctttt ttctcgaaaa tccgaattca
      961 tcctcaagaa atgcaactgc tggctggatc attgacaccg atgaagttat tcctgcaata
     1021 aaaaggagca taaaccacaa gaatgcgaag aaaggcccgc ccggcatatt tgcaaaaata
     1081 ttgggcattg ttacaaaacc tatgttaaaa gctccggtag ttgctatcct gacagtttcg
     1141 gcagaaccga aaaatatgaa tgcggcggga attatgagtg accctccgag aattacttcg
     1201 cagaattcat ttgttgctga agccgtaagt cctgacaacg ctatatcatc atttttctta
     1261 agatagctcg cataggtcat tatcactccc attccaagtg acaaagtgaa aaatatctgc
     1321 cccgccgcac tgagccacac ttttgaattg agaagaacag tgaaatcggg tgtccataca
     1381 aaattaagtg cgtttgaaac agactgatcg ggcttcaacg gatcgggagt gccgagcgtc
     1441 aatatcctga tgacaagcag aactgcaatt attacaagaa gaggcatcgc gatttttgac
     1501 agtttttcaa tacctgaggc cacacctttg tatataaaat agaaattaat gatgatcgtc
     1561 accacaaaaa atccgtaagc tatccaaatg gaatcaaaat aaccgtttga gacaacaccc
     1621 tgataacctt gaagaaactg agcaagttca ttttttccgg caacatctgc aagggttccg
     1681 cttatcgaaa agaacgcata ggcaagagac cacgattcaa tgaaagcata gtaaaaaagg
     1741 atgagcagag gaatgattat ccctattgaa gcaaaatatt taataaatct gctgccagtt
     1801 atcttgtaaa gtattgcagg agctgtgccg tgaccgtact cgcccccttt tcttccaagc
     1861 gaccattcaa cccacatcag cggaatgccg agcagaagga atgaaacgaa ataaggaatc
     1921 ataaaagctc cgccaccgcc aagagccgcc tgtgtcggaa atctcaaaaa attgccgagt
     1981 ccgactgcac ttcctgcgac tgcaagaatt acaccgagct ttgtccccca ctgttctctt
     2041 ttcggtgatg acattgaacc tcctctaagc taaaagactt atggttactt aagcatctcc
     2101 tcgccggaaa aatcgcggta aagtgtaagc tgatcgagta tgaagttgta ttcggttgag
     2161 ttgtcaataa agatctcaga tagtattctg ccaagtcccg gtccgagcat aaagccctgt
     2221 ccgcacattc ctactgcatg aaagacattt tctatttcgt gtgccattcc gacgatcggg
     2281 aacccgtccg gagtcatcgg ataaagtccg cgccatgtcc ttctcaccct cagattcctc
     2341 aaacggggat acagttcaag catccttttg atcatgagcg gaagaaattc gctagtatta
     2401 tcagtatctt tgcctgccgc tttgggataa ggagttatgc agaaaacaac ctgtccttca
     2461 atattctgat aaaaataata gttgtgtgaa tgggaatcag gtctgatgtc aacgaccatc
     2521 ggtttaaaga acggtttcac cggctcagtt attcccgctt catgggaatc aggataaacc
     2581 ggaaggtcca ctccgtacat tttgccgatc tcttttgcat ctccccctgc cgcattgatg
     2641 ataagacctg cactgtatgt ctctttgttt gtcttcacac ttacaacttt tttatctttc
     2701 acttccatcg aaaggacatt ttcataaaac ctgaattcgg ctccggcctt tgtcgcaagc
     2761 ttgaagtatg ctcctgtagt tttaagcgga gacgcactgc cgtcatttgg tgaaaatgtt
     2821 ccgccccgaa gcccgctttc cgttatcccc ggaacaagtg ccttcaatgc gtcggcatct
     2881 atccagtcaa tgttcagccc gaactttttc tgaaccttga gaagatcttt gagatttctt
     2941 tcccgttggg tgtcataaac ggggaaaagg tatccccctt ctatccagtc gatatctatg
     3001 ccgtgatctg aatgcatgtt tctgatctct tcaagtgaca tcgtgcatat ttttatcttt
     3061 gcaggatcgc tgtgtgtcgc ccttattccc cctattgccg ctctgttctg tcctcttccg
     3121 gtcgaagcgt gtttttcaag aacgcagaca cttttgcctt tttttgcaag atgataagca
     3181 agaggagttc cgatactgcc tgcacctata attaaaaaat cgtatgtttt catgttactc
     3241 tccatcctcg ttgatcagtg catacatcgg agtttcgaca cacatcggtc tgttggtccc
     3301 ttcagtaact tccgaccagt tcacccctgc cgccttgaaa actctcggca tctggacact
     3361 gcatgtctta gacccgcatg cacccattcc gaccctgatc tgcttaagct ggttaatgtc
     3421 tctgatctta ttttccttta taaaatcaac cacttccttc agagtcaccc tttcacagcg
     3481 acagacaatg ccgttttcag ggatatattc gtaatcagca gttttcagcg gacgagtcac
     3541 agattcatcc tgaaccctta tccccataac ttttgccgca ttgtttaatg aaacagcaac
     3601 cgtgataaga gctgtcttat attttttgtt gaatgttatc tttgttattt ttccatcttc
     3661 aagatagctg ccgtccatat cggtaagagg aatcatgtcc cccacgacaa agtcacagtt
     3721 gaattcatgg ggaagcatca cttcagccgt tttttcatca acctttttca caagggttat
     3781 cgccagtccg ggacatgcgg cgacacatat tccgcaaccg atgcattcgc cggcaagata
     3841 aggaatatcc attatcgtcc ctgacttatc catcagtttt attgcatttt tcgggcatac
     3901 cgttgtgcat ggattgcaag ggatctcctg aagacagctg aaattcggtt ggaacttttc
     3961 tgccatttta acaggattca gcggatgaac tataccggga cgcgatttga gaacttcggc
     4021 tttcttatac catgtgtcat ccacaacaac atcttttctc ccaaggagcg ccgccatttt
     4081 aagccctgcg atacggccgc cgaacattgc cgaacttgct tcagcgatct cttcggcatc
     4141 tcctgctttt ataacattga actcaaattt ttcagccatg tcgaaaaatt catcagtcgg
     4201 ggtcagtccc gccgcgacaa gaagtgtatc aacttcaaag gttcttgccg tctcaagtac
     4261 agggttccac gcactgtcaa cttcagcaat agtcaccctt tcaacttttc ctttcccttc
     4321 agcacagaga actgtggtgt tgagataaag aggaacgccc attcttttta tcttgtcggc
     4381 atgaacttta tatccgctta tttcgtgcgc tatttcgaca attcccgcca cttcgatgcc
     4441 tgcctgaaga gcgtgatatg caccgatgag accgacattt ccgcttccga tgatgagaac
     4501 tttgtcagac gccttgacca gatcacggtt cacgagagtc tgaaaagctc ctgctccgta
     4561 aactccggga aggtcgttac ctgggaaaac aattgacttc tcccttgctc cggcagtaat
     4621 gagaacccct ttgaagtcaa caagaacata gtgtctgttc tcaacaaaaa ggcctgcttt
     4681 cttatctttg taaaccccta caattgaagt ttcagtgaat attttcacat tgctgtattt
     4741 tctcacttcc tcttcaagaa ggttggcaat gtcaattcct cttgtccctg cataacaatc
     4801 ctcttcactc ccgaagaatt tgtgggtctg gagaagaagc ttaccgccga gtttatcctt
     4861 atcgtcaaca agaacaaccc tgaaaccgag ttttgcaagt tcgatcgtgc tcgtaagtcc
     4921 gctcggaccg ccaccgatga caagaacatc gcaaaccaca cattttcttt cgtatcccgc
     4981 catcggagaa tcatctgcag gaagatctgg aaggtgtttc atcgttctta catccatccc
     5041 ttcctttaat ggtgtgatac aagatttcag aggccttccg tcaatgatta cagtacattg
     5101 actgcactgc ccgtttgcac agaaaatccc ctgaggagca tcacccttcc tgtgaattga
     5161 gaactgtttc actccgttgg caaacagagc tgaactaacc atctcatctt caaacccttt
     5221 catctgagtt ccgtcaaaca tgaaagaaac ttcatgtctt tctttcggaa cattcaaaac
     5281 cggatgcact ttgattctgt tcattgtctc accatatata ccttaataat taaaaaaact
     5341 aaaaaaacag ggcagaataa ttcttgaaag tttaaatgtc aagacgaaga agggtt
//
LOCUS       DAOOYB010000194         5345 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_17851_length_5345_cov_10.713043, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000194 DAOOYB010000000
VERSION     DAOOYB010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5345
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17851_length_5345_cov_10.713043"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="PL056_10410"
     CDS             complement(<1..188)
                     /locus_tag="PL056_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HPM47467.1"
                     /translation="MFFRNRKWALVLSGGGAKGIAHIGVLKFLDEHKLRPDMIAGTSA
                     GAIIGSFYCCGMTALEIE"
     gene            complement(574..3069)
                     /locus_tag="PL056_10415"
     CDS             complement(574..3069)
                     /locus_tag="PL056_10415"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HPM47468.1"
                     /translation="MFSLKKRDGSLVTFDLVRIKEAIKKAFDATEMIYNEDILDILSL
                     RVTANFQSRIKDDVIMLEDVQDCVEKTLEQTGYTEVAKAYILYRKQREKLRNMKSTMV
                     DYKNIINSYVNNEDWRVKENSTVTFSVGGLILHNSGAVTANYWLSEIYDEEISRLHKN
                     GDFHLHDLSMLTAYCAGWSLKQLIETGLGGVTGKITSAPASHLYTIANQMVNFLGIMQ
                     NEWAGAQAFSSFDTYLAPFARVDGLNYKEVKQAIQAFVFGVNIPSRWGTQSPFSNITL
                     DWICPDDLKNLPAIVGGKEQKFTYGDCQKEMDMVNKAFIEIMIEGDAEGRGFQYPIPT
                     YNITSEFDWESDNAKLLFEMTAKYGTPYFQNFINSDMKPGDVRSMCCRLQLDKRELLK
                     KTGGLFGSGEKTGSVGVVTINLPRVAHNAKNEAEFYSRLQYLMGAAKRSLEVKRTVCT
                     KLLEQNFYPYTKRYLGNFNAHFSTIGLIGMNEMCLNASWVKQNIASQKGLKFSQDVLD
                     FMREELKRYQEETGNLYNLEATPAESTTYRLAKMDRERWPEIKTASIGKEKPFYTNSC
                     HLPVDYTTDPFEALEIQDQLQTRFTGGTVFHTFLGEKLPSWEAAAKLVRKISYTFKLP
                     YFTISPTYTICNKCGYFAGEHDMCPKCGDKVEVYSRITGYYRPIEHWNEGKKQEYEKR
                     KEYVPEHEKMRKISDSGNHVFKRGERVETAQETLFTTGNGKLSDEISRSNVSHSSLIN
                     PGLGSGVDPEIRFQQKVMLFTTKHCPNCPRAKEEMQGTTNLQVIDAEENFDVTRKYGI
                     RSVPAVVVEEAGAYKTYTGISDIIKYKRELNLR"
     gene            complement(3214..4737)
                     /locus_tag="PL056_10420"
     CDS             complement(3214..4737)
                     /locus_tag="PL056_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HPM47469.1"
                     /translation="MFTILKSGSISGIEGSVIEVEVDISKGLPGFSIVGLPDSAVKES
                     RERVRTAIINSGHKFPQNRITINLAPAGIKKEGAGFDLPIAAAIISSIYSMEPEKINR
                     YMIAGELALNGDIKPVRGVLPLAITAKESGLSGVIVPAENGDEAAAITEINVVTVKNL
                     GDILELLKEGPVNFHKTSDVRIEKKHIYDFSEVRGHEQAKRAIEIAAAGGHNILMVGS
                     PGSGKTMLARRIPTILPELEREESIETTKLFSVSNLLQGKGLINERPFRSPHSSCSHI
                     ALVGGGSPPKPGEVSLAHNGVLFLDELPEFNRNALEVLRQPMEDGEVTISRAVHTIKY
                     PSSFMLVASMNPCPCGYLFDIKKTCTCSSSAVHKYQQKISGPLLDRIDISIVVNSVPY
                     QDLRAKGTGEKSDEIRARVVKARSIQKKRFEGMSFYTNARMQASEIDKYCMLDESAEK
                     LLKNAMEKMGISTRGYSRILKVGRTIADLADKEKIEASHIAEAIQYYNRTMFTDSVF"
     gene            4984..>5345
                     /locus_tag="PL056_10425"
     CDS             4984..>5345
                     /locus_tag="PL056_10425"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HPM47470.1"
                     /translation="MNIIKTLRVCYRVVLILLWTLFMQNIFIDIPRLFSKKRRFPRII
                     HIWGKGLAWLMGIKIHRKNNLPEPMGEVIISNHLGFLDVPIMSSIFPAVYMIKAEAKK
                     PFYFGPALSREGHFFVDR"
BASE COUNT         1387 a         1225 c          983 g         1750 t
ORIGIN      
        1 tcctctattt caagagctgt catgccgcag cagtaaaaac tgccgataat tgcacctgca
       61 gaagtccctg caatcatatc aggtctcagt ttgtgttcat caagaaattt caaaaccccg
      121 atgtgggcga tcccctttgc tcctccacct gacaacacaa gagcccattt tctgttcctg
      181 aaaaacattt tttctcctta aaacaaaaaa acctgctgat tttatactta ttcaaattat
      241 ttcgcaaact ttaataagaa aagcaataac caaacaattt cagcaccttg gcggtcttga
      301 tttatcaatc cgaacctgca cttgattttt ttccggttct actttaacga aatggacgaa
      361 tctctttttc ggttaaatta ttatcaatgc gtaaaatggt tttttcttaa atgaaaatat
      421 gatcctcaca cacacaaacg ctttcccgct attcaaaaag cggaagcgga tcaacttgcg
      481 aaaatttagg ggtgctgcca atccctcccg acacacaatc ttgcggcacc cctttcgctt
      541 ttttactaca ttatttttca gaattttttt gaattatctt aaattgagtt ctcttttgta
      601 tttgatgatg tcagatattc ctgtgtatgt tttgtatgca ccggcttctt caaccactac
      661 cgcaggaacc gatctgatgc cgtattttct tgtaacatca aaattttcct ctgcatcaat
      721 gacctgaaga tttgtcgttc cctgcatttc ctcttttgct ctcggacagt tcgggcagtg
      781 ttttgtcgta aaaagcataa ctttctgctg aaatctgatc tcagggtcaa ctccgcttcc
      841 tagccccgga tttattaaag aactgtgaga aacattagac cttgatattt catcgctcaa
      901 cttgccgttt cctgtcgtaa aaagagtttc ctgagctgtt tcaactctct ctcctctttt
      961 aaagacatga ttccctgaat cagagatctt tctcattttc tcatgctcag gaacatattc
     1021 ctttctcttc tcatattcct gctttttccc ttcattccaa tgttcgatcg gcctgtagta
     1081 tccggttatc ctgctataaa cctcaacttt gtcgccacat tttggacaca tatcatgttc
     1141 acctgcgaaa tatccgcatt tgttacagat ggtgtatgtg ggcgagatcg taaagtaagg
     1201 aagtttgaaa gtgtatgata tctttctgac aagtttcgcg gcagcttccc atgaggggag
     1261 cttttcgccg aggaatgtgt ggaaaactgt tccgcctgta aatcttgtct gaagctgatc
     1321 ctggatctca agtgcttcaa acggatctgt cgtgtagtca acaggaagat gacagctgtt
     1381 ggtgtagaaa ggtttctcct tgcctattga ggcagttttg atctcgggcc atctttccct
     1441 gtccatcttg gcaagacgat atgtcgttga ttccgccgga gttgcctcaa gattgtaaag
     1501 attgcctgtc tcttcctgat atctcttcaa ttcctctctc ataaaatcaa gaacatcctg
     1561 agagaatttc aaccctttct gagatgcgat attctgtttt acccatgatg cgttaagaca
     1621 catttcattc attccgataa gacctattgt tgaaaaatgg gcgttgaaat taccgagata
     1681 tctttttgtg taaggataaa aattctgctc aagaagtttc gtacagacag tccttttaac
     1741 ttcaagactt ctctttgcgg cacccataag atactgaagc cggctgtaaa actctgcttc
     1801 attctttgca ttgtgtgcaa ctctcggaag gtttatggtt acaacaccga cgcttcctgt
     1861 cttctcaccg cttccgaaaa gtccgcccgt ctttttgaga agctctctct tgtcaagctg
     1921 aagccggcag cacatacttc ttacatcacc cggtttcatg tcactgttga taaaattctg
     1981 aaaataagga gtgccgtatt ttgctgtcat ttcgaaaaga agctttgcat tgtcactttc
     2041 ccagtcaaat tcacttgtaa tgttgtaagt cggtatcgga tactgaaatc ctcttccttc
     2101 agcatcacct tctatcatta tttcaatgaa tgctttgttt accatgtcca tttctttctg
     2161 acagtcgccg tatgtaaatt tctgttcctt tccaccgact attgcaggaa gatttttaag
     2221 gtcgtccgga cagatccagt cgagagtgat attgctgaaa ggagactgtg ttccccatct
     2281 tgacggaata tttacgccga aaacaaatgc ctgtattgcc tgtttgactt ccttataatt
     2341 caatccgtca actctggcaa aaggggcaag gtatgtgtca aaacttgaaa aagcctgtgc
     2401 acccgcccat tcgttctgca taataccgag aaaattgacc atctggtttg caattgtata
     2461 aagatggctt gccggagctg aagttatttt tcctgtaaca ccgccgagcc ccgtttcaat
     2521 gagctgttta agtgaccagc cggcacagta tgcggtaagc attgacagat catgaagatg
     2581 gaaatcgcca tttttgtgaa gtcttgagat ctcttcatcg tatatttcac tcagccagta
     2641 gtttgcagta acagcgccgc tgttgtgaag aatgagcccg ccaacgctga aagtcacagt
     2701 tgagttttcc ttgaccctcc agtcctcgtt atttacatag ctgtttatga tatttttata
     2761 gtcaaccatc gttgacttca tgtttctgag cttttctctc tgctttctgt aaagaatata
     2821 agcttttgcg acttcggtgt aaccagtctg ctcgagtgtc ttttctacgc agtcctgaac
     2881 atcttccagc atgatcacat cgtctttgat cctggactga aaattcgcag taacacgcaa
     2941 agagagaata tcaaggatat cctcgttgta aatcatttct gttgcatcga atgccttttt
     3001 gatagcttct ttgatccgga caagatcaaa agtaacaagc gaaccgtctc ttttctttag
     3061 actgaacatc aataaatctc cgccagataa atagttttat aattccgacg aggagtaaat
     3121 tacactatcc cttgtaggtt gtcaacaaaa aacacactag atattgtatt tttttgcaag
     3181 ttattaaaat cagtgatgtt tctttaaaat gctctaaaaa accgaatctg taaacattgt
     3241 tctgttgtag tactgaatcg cctctgcaat atgtgatgcc tctatcttct ctttatccgc
     3301 aagatccgca atggttctcc ccactttcag gatcctcgaa tagccgcggg tgcttatacc
     3361 cattttttcc attgcattct tcaacaattt ttccgcactt tcatccagca tgcagtattt
     3421 atctatctct gacgcctgca ttcttgcatt ggtataaaaa gacattcctt caaacctttt
     3481 cttctggatc gacctcgctt ttaccactct tgctcttatt tcatcacttt tctcaccagt
     3541 gccttttgcc cgcagatcct gatagggaac agagttcacg actattgaga tatcaatcct
     3601 gtcaagtaac ggtccggaaa ttttctgctg atatttatga acagccgaag aactgcaggt
     3661 acatgtcttt tttatgtcaa aaagataacc gcatggacag gggttcattg aagccacaag
     3721 cataaaactt gaaggatatt ttattgtatg caccgctctt gaaatggtta cttctccatc
     3781 ttccatcggc tgtctgagca cttcgagtgc gtttctgttg aactcaggaa gttcatcaag
     3841 aaaaagaact ccgttatgtg caagactgac ctcaccgggt ttaggcgggc ttcctcctcc
     3901 gacaagtgcg atatgtgaac aggacgagtg cggacttctg aacggtcgtt catttatgag
     3961 tccttttccc tgaagaagat ttgaaacact gaaaagtttt gttgtctcaa tgctttcctc
     4021 tctttccagc tcaggcagaa tagtcgggat cctccttgcg agcattgtct ttccgcttcc
     4081 gggagaacct accattaaaa tattatgccc gcctgcagcg gctatctcta tggctctttt
     4141 tgcctgttcg tgtccacgca cttctgaaaa atcgtaaata tgtttctttt caattctcac
     4201 atctgatgtt ttgtgaaaat taaccggacc ttcctttaat aattccagta tatctccaag
     4261 atttttaaca gttacaacat ttatttcggt tattgcagcg gcttcatccc cgttctcggc
     4321 cggaactatc actcccgaaa gccccgattc ttttgccgtt atggccaaag ggagaactcc
     4381 cctcaccggc ttgatatcgc catttaatgc aagttctccg gctatcatat atctgtttat
     4441 cttttccggt tccatggaat aaattgaaga tattatggcc gctgctatcg gaagatcaaa
     4501 tcccgcccct tcctttttaa ttcccgcggg agccagattt atcgttattc tgttctgcgg
     4561 gaacttatgt ccactattta ttatcgccgt cctgaccctt tcccgcgact ccttcaccgc
     4621 gctgtcagga agacctacta ttgaaaatcc gggaagccct tttgaaatat ctacttcaac
     4681 ttctattaca cttccctcta ttccggatat tgaaccgctt ttgagtattg tgaacattag
     4741 cacctctttt aaaacaactc gctttattat atctctaatt tctgtatgtc aaacttttgt
     4801 tataatcatt ttagcgaagg gaacaaattg tctttgcttt taatttatta atgttttata
     4861 aatgctttat aattgtgtaa tataaaagtt gatttttctg taaccggagg ataaattcgc
     4921 aagtcatagt gacgaccggg aaaacgaaca ccggtggtta ttgataaacg gctaaggcaa
     4981 aaaatgaata taatcaaaac gttaagagtt tgctacagag ttgttctgat actgttgtgg
     5041 actctgttca tgcagaacat tttcattgat attccaagac tgttttcaaa aaagaggcgg
     5101 ttcccccgca taatacatat ctgggggaaa ggtcttgcgt ggcttatggg catcaaaata
     5161 caccggaaga acaaccttcc tgaaccgatg ggcgaagtaa taatatccaa tcacctcggc
     5221 tttcttgatg tgccgataat gtcaagcatt tttcctgccg tttatatgat aaaagcggaa
     5281 gcaaaaaaac cgttctattt cgggccggca ctttcaaggg aaggacactt ctttgttgac
     5341 aggga
//
LOCUS       DAOOYB010000195         5339 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_17878_length_5339_cov_9.427896, whole genome shotgun sequence.
ACCESSION   DAOOYB010000195 DAOOYB010000000
VERSION     DAOOYB010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17878_length_5339_cov_9.427896"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="PL056_10430"
     CDS             complement(<1..334)
                     /locus_tag="PL056_10430"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF024897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HPM47471.1"
                     /translation="MKKANVLILTGYGINCDVETGWAFEMAGADKFERVHVNRIINGE
                     VNPDDFQIMVFPGGFSFGDHIASGRVLGLKVKKQMGDKLKKFILDGKLVIGICNGFQT
                     LVKMGVLPG"
     gene            complement(344..3343)
                     /gene="purL"
                     /locus_tag="PL056_10435"
     CDS             complement(344..3343)
                     /gene="purL"
                     /locus_tag="PL056_10435"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HPM47472.1"
                     /translation="MVFRVEVGLRKDMKDPEGATWLRRIHNDLNIEKVSDVRVLKVYL
                     IDCEGIDIKQVNSFANEVLADPVTEVFETSEILADNYQFDWLIETGFLPGVTDNEGKT
                     ALWGLKTFLGSIGDDDMIFSGRKYLVTGRVEKNTAEIIGSKLFANGLIQRYSVVSFQE
                     WAGGKKIGNTVPRVKSSSVPVVETVALDLSDEELMALSRSKVLALNLDEMKAMRDYFR
                     KKEVLEYRKQKGFPKKATDCEIEVIAQTWSEHCKHKIFAADINYSDKVNKRDMQIRSL
                     YKSYIKKVTYELRESKPWIKSVFDDNAGIIQIDGDTLFAMKVETHNSPSALDPYGGAL
                     TGIVGVNRDIIGCGIGAKPILNTDVFCFASPYYEGTLPGSNLMHPARIFRGVHRGVKD
                     GGNESGIPVVNGSIFFDNRYIGKPLVFCGTGGLMPVEINGHPAHLKKARIGDLIVMSG
                     GRVGKDGIHGATFSSEELHEGSPVTAVQIGDAIVQKRMLDFIIEARDKELYNAITDNG
                     AGGLSSSIGEMARDTGGAEINLEKVPLKYQGLQPWEIFISEAQERMTIAIDPARFDEL
                     KKIADIHEVEISVVGKFTDTGYLEIYYEDMKVALLDMEFMHDGNPRYKLEAEWDFVEK
                     PAEEIEDQENLNAVLLDMMGRVNIASKENWVRQYDHEVQGRSAGKPFCGVEHDGPSDA
                     GVLRVSPFKKNAIVVSHGIKPTFSDIDCYWMTASVVDEAVRNAVATGADPDFMSGLDN
                     FCWPDPVYDSDKNPDGKHKLAQLVRSNMALYDFCRYYSIPLISGKDSMKNDYGSGKNK
                     ISIPPTLLFTLISKMDDVENMVSMDFKNEGDRIFIIGETKNELGGSEYYIYHGINRDG
                     FIPKVDAGKFMRSYKLLKKAIDSKLVKSAHDLSDGGFATALSESAFAGALGAKVDLRK
                     IPRSVDVTRNDTLMFSESNGRIIVSVAPANVEKFLEVMKGTDMDEIGEVTSSGFVKMT
                     GLDGSVVVNLSINELKKNWKGLMSRMN"
     gene            complement(3542..4390)
                     /locus_tag="PL056_10440"
     CDS             complement(3542..4390)
                     /locus_tag="PL056_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HPM47473.1"
                     /translation="MNGIILFEQKKVRRVRFNDEWWFVINDVVAVLTDSVNPSDYLKK
                     LRKRDESLSDAFKGGGQSVPPLGLEFDTAGGRQMLQCWNTTGILRLIQSIPSPKAEPF
                     KRWLAKVGYERLEEIENPELAAKRMREIYKQKGYSDEWIEKRMRGIAVRDELTDEWKK
                     RGVKEKAEYSILTAEISKATFGMTPSEYKDFKELTNPGDNLRDHMTDLELIFTMLGEA
                     STTEIAREKDAQGFTENKEAAVKGGVVAGNARRELEQKSGKKVSTRQNYKQIPQSKSK
                     GTKKIK"
     gene            complement(4624..>5339)
                     /locus_tag="PL056_10445"
     CDS             complement(4624..>5339)
                     /locus_tag="PL056_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HPM47474.1"
                     /translation="EKTDDPEEIVVFPDPGLEKCVRKAIMKSEGDILLEDLKYVFSLK
                     YCEGFEIRDLTGIEKITVLQSFQAGNNKIKDLTPLSKCLNLRELDLYNNEITDGSPLK
                     NLANITRLNISNNSISDISYVKDMVNLNSLYTGGNPISDISPVANLASLTALNFGNSN
                     VSEIKDVAKLINLNVLGIKNNKIKDISSIKLLKNLVTIDVRENCITNFSPIDYLKENG
                     SLTSVQGDSVSDQDYDRCK"
BASE COUNT         1357 a         1309 c          934 g         1739 t
ORIGIN      
        1 ctccgggaag cactcccatc ttcacaagtg tctgaaatcc gttacatatt ccgataacga
       61 gttttccgtc aagaatgaac ttcttcagtt tgtctcccat ctgctttttg actttcagac
      121 cgagaactct gcctgaagca atgtggtcac cgaaagaaaa accgccgggg aataccatta
      181 tctgaaaatc gtcggggttt acttcaccgt ttattatcct gttgacatgg actctttcga
      241 atttgtcggc acctgccatt tcaaatgccc agcccgtctc tacatcacag tttataccat
      301 agccggtgag tataagtaca tttgcttttt tcattttttc acctcagttc atacggctca
      361 taagcccttt ccagttcttt ttaagctcgt taatgctcaa attgacaact accgatccgt
      421 caagtcccgt catttttaca aaaccgcttg aagttacttc gcctatctcg tccatatctg
      481 tgcccttcat cacttcaagg aacttttcaa cattggcagg ggcgactgaa acaattattc
      541 tgccgttgga ttcactgaac ataagagtat cgtttctggt aacatcaacg cttctgggga
      601 tctttctcag atcaactttc gctccgaggg ctcccgcaaa agccgattct gaaagagcag
      661 ttgcaaagcc gccgtcagaa aggtcgtgcg cactttttac aagcttgctg tctatcgcct
      721 ttttcaggag tttgtaactt ctcatgaact ttcctgcatc aactttggga atgaaaccat
      781 ccctgtttat tccgtgatag atgtaatatt cactgcctcc aagttcgttc ttcgtttctc
      841 cgatgataaa tatcctgtcg ccttcattct tgaaatccat tgacaccata ttttcgacat
      901 catccatttt tgaaatgagt gtgaaaagaa gcgtcggcgg aatagagatc ttgttcttgc
      961 ctgaaccgta atcattcttc atgctgtcct taccgctgat gagcggaata gaatagtatc
     1021 tgcagaaatc gtaaagcgcc atgttgcttc tcacaagctg tgccagtttg tgcttcccgt
     1081 caggattctt gtcactgtcg taaaccgggt ccggccagca gaaattatca agtccgctca
     1141 tgaaatcagg atcggcaccc gttgcaacgg catttctgac cgcctcatca acaacagagg
     1201 cagtcatcca gtaacagtct atatcgctga atgtcggttt tattccgtga gaaacgacta
     1261 ttgcgttctt tttaaaaggt gaaactctaa gaacgcctgc gtcgctcgga ccgtcatgct
     1321 caacaccgca gaaaggtttt cctgcgctcc tgccctgaac ttcgtgatca tactgtctca
     1381 cccagttctc ttttgaagcg atgttcaccc gtcccatcat atcaagcagt accgcattca
     1441 gattttcctg atcttcaatt tcctcggccg gcttttcaac aaaatcccac tctgcttcaa
     1501 gtttatatct cgggttgccg tcatgcatga attccatgtc aaggagagca actttcatat
     1561 cttcataata aatttcgaga tacccggtat cggtgaactt tccgacaact gatatctcga
     1621 cttcatgaat gtcggcgatc tttttcagtt catcgaatct tgccggatct attgctatgg
     1681 tcatcctttc ctgtgcttcg gaaataaaga tctcccatgg ctgaagtccc tgatatttca
     1741 aaggaacctt ctcaagattt atttctgctc cgccggtatc acgggccatt tcaccgatac
     1801 ttgaactcaa tccgccggca ccgttatcgg ttattgcgtt gtaaagttcc ttgtctcttg
     1861 cctcgattat aaagtcgagc attcttttct ggacgattgc atcaccgatc tggacagctg
     1921 tcaccggact tccttcatga agctcttcac tgctgaatgt cgctccgtga attccgtctt
     1981 tacccactct tccgccgctc attacgatca gatctcctat tcgggctttt ttgaggtgtg
     2041 ccggatgtcc gtttatctca acaggcatca atccacctgt tccgcagaat acaagcggct
     2101 ttccgatata tctgttatca aagaatattg atccgttcac gacagggatg ccgctttcat
     2161 tgccaccatc cttgacacct ctgtgcactc ctctgaaaat tctcgccgga tgcatcaggt
     2221 tgcttccggg cagagtccct tcatagtaag ggcttgcaaa gcagaataca tctgtgttga
     2281 gtatcggttt tgcacctatt ccgcaaccaa tgatgtctct gttcactccg actatccctg
     2341 tcaaagctcc gccgtaggga tcaagtgcgc tcggagagtt gtgtgtctcc actttcattg
     2401 caaaaagggt gtcgccgtca atctggatta tcccggcatt atcgtcaaaa accgatttta
     2461 tccacggctt tgactctctg agctcgtatg tcactttttt gatatatgac ttgtaaagcg
     2521 atcttatctg catgtctctt ttattgacct tatccgaata atttatgtca gcggcgaata
     2581 ttttgtgttt gcagtgctca ctccatgtct gggcgatgac ttctatttca cagtcagtcg
     2641 cttttttggg aaaccctttc tgctttctgt attcaagcac ttcctttttt ctgaagtaat
     2701 ctctcatcgc cttcatttca tcaaggttca gcgcaagaac tttcgacctt gaaagtgcca
     2761 tcagctcttc atcggaaagg tcaagtgcca ccgtttcaac aactggaacc gaactgcttt
     2821 tcactctcgg aacggtgttg cctatcttct ttccccctgc ccactcctga aaacttacga
     2881 ccgagtaacg ctgaatgagc ccgtttgcaa aaagttttga accgataatt tcagcagtat
     2941 ttttctccac tcttcctgtg acaagatatt ttcttcccga aaagatcata tcatcatcgc
     3001 ctattgatcc aagaaaggtt ttaagccccc acagagccgt ttttccttcg ttatcagtaa
     3061 cgccgggaag gaatccggtc tcgatgagcc agtcgaactg atagttgtct gcaagtatct
     3121 cgcttgtctc aaaaacctct gtcacgggat ctgcaagaac ttcatttgca aaactgttca
     3181 cctgtttgat gtctattcct tcacaatcta tgagatagac ctttaacacc ctgacatcac
     3241 tgactttttc aatgttaaga tcattgtgta tcctccggag ccaggtcgcc ccttccggat
     3301 ccttcatgtc ctttctcagt ccaacttcaa ctctgaaaac cattatctcc tcctgacaat
     3361 tcagataaaa ctatcgagcc gccggaatag tattaaaaac tgttacgata ttcaattttt
     3421 taatttaatt tccgttttct ttgagataat caatagggga aaaatcgttt atgcagttat
     3481 cttcgatatt tatggaaaca atttggtttc catgtaatga taaaatataa aaacgatcgg
     3541 gctatttaat ctttttagtt cctttcgact ttgattgagg aatttgttta tagttttgac
     3601 gggtagatac ctttttaccg gatttctgtt caagttctct tcttgcattt ccggccacta
     3661 cgcccccttt aactgcggct tccttgtttt cggtaaatcc ctgagcatct ttttctcttg
     3721 ctatctcagt tgttgaagct tctcccagca ttgtaaaaat aagttcaaga tctgtcatat
     3781 gatcacggag attatctccg ggatttgtaa gctccttaaa atctttatac tctgaaggtg
     3841 tcattccgaa agtcgctttg cttatttcag ctgtaaggat ggaatattcc gctttctcct
     3901 taacgcctct ttttttccat tcatcagtca attcgtcacg gacggcaatc ccccgcatcc
     3961 tcttttcgat ccactcgtcg gaatatcctt tctgtttata aatttcacgc atcctttttg
     4021 ctgcaagttc cggattttca atctcttcaa gccgttcata tcccactttt gcaagccatc
     4081 tcttaaaagg ttcagctttc ggagatggaa ttgactggat cagacgcaga atgccggtag
     4141 tattccagca ttgcagcatc tgtcttccac ctgctgtatc aaattcaagc ccaagggggg
     4201 ggacagattg tcccccccct ttaaaggcat cactcaaaga ctcatctctc ttccggagtt
     4261 ttttcaaata atctgacgga ttaactgaat cggtaagtac cgctacaaca tcatttatta
     4321 caaaccacca ttcatcatta aacctgactc tccgcacctt tttctgctca aataaaatta
     4381 ttccgttcat cacattctct ccatcttttc aaaaaactga tccgttgaat attaactgat
     4441 aaaatgagta aatacaaaat taaagaaagg tcattcgaca ccatcacgaa gtgaccaacc
     4501 attccacgat tacaccatta cacctgtcat gagcttgtca aaacgcattg aatcctgaaa
     4561 caaccgctgg atgactctgg ggaagaaaga tcgtgcggta actggattta atcagaacat
     4621 cgtttattta catctgtcgt aatcctgatc actgacagaa tcaccttgca cagatgtcag
     4681 acttccattt tcttttagat aatcaatagg ggaaaagttg gttatgcaat tttctcttac
     4741 atcaatagtt actaaatttt ttagaagctt tattgaacta atatccttta tcttgttgtt
     4801 ttttatccca agaacattta gatttattaa ttttgccaca tcttttattt ctgatacatt
     4861 agaatttcca aaattaaggg cggttagact tgccagattt gctaccggtg aaatatctga
     4921 aatcggattt ccaccagtat ataatgaatt taaattcacc atatccttaa catagctaat
     4981 atcagagatt gaattattcg aaatattcaa tcttgtgata ttagctaaat ttttcaaggg
     5041 agatccatct gttatttcat tattataaag atccagttcc ctcaaattca aacactttga
     5101 caacggggtt aaatctttga tcttgttgtt gccagcttga aaagattgca gaactgtaat
     5161 tttctcaatt cctgtgagat ctcttatctc aaaaccttca caatatttta gagaaaacac
     5221 atattttaaa tcttctaata aaatatcccc ttccgatttc ataattgcct ttctaacaca
     5281 tttctccagt ccgggatcag ggaaaacaac tatctcttcc ggatcatctg ttttctcgc
//
LOCUS       DAOOYB010000196         5330 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_17931_length_5330_cov_9.751280, whole genome shotgun sequence.
ACCESSION   DAOOYB010000196 DAOOYB010000000
VERSION     DAOOYB010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5330
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17931_length_5330_cov_9.751280"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..963
                     /locus_tag="PL056_10450"
     CDS             97..963
                     /locus_tag="PL056_10450"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006922035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HPM47475.1"
                     /translation="MAFIKLLRPHQYIKNLLVFFPLFFALKINNIALLKQSLIAFAAF
                     CFAASTIYIVNDIRDIEKDRAHPEKKNRPLASGKVSVKNAIVLSALLFLAAVAVSYFG
                     GLNLFLIIGFYILMNIGYSFGLKNVPLIDIFIVSTGFLLRLLAGTHFGGIEGINPSHW
                     IIIMTFLMSLFLAFAKRRDDLVLAEKGVEVRKSISGYTIEFINNAMSVMSAVLIVSYL
                     LYTLDAKVQNHFQTENLYATTLFVILGVFRYMQLTFVFHKSGSPTDIVYKDRFLQLTI
                     LGWLLMFLYFAY"
     gene            976..2013
                     /locus_tag="PL056_10455"
     CDS             976..2013
                     /locus_tag="PL056_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPM47476.1"
                     /translation="MKVLITGGAGFFGLHMTKKCVDMGEEVTLLDIAEYDKAEYDKSV
                     KLVKGDVRDAEIMDKLISENDVVIHAAAALPLEKKKEIFSTNVDGTRNVLELCFKHKT
                     RKFVMISSTAVYGVPDHHPLFETDKMIGVGPYGESKIAAEKICSEYRDKGLFITTVRP
                     KTFIGTHRLGVFQILYNWVEEGHRIPMIGNGKNRYQLLEVDDLCDAVYLAYTSQNELA
                     NGVFNIGAVSNLSANDYLGALCEYAGSGSTILHTPVSLVIPALMLFEWLHISPLYKWV
                     YGTAHKDSFVSVDKAREVLGWNPKYDESQALIRSYQWYQDNKAYIAKGTGTSHRVPWA
                     QGILGIIKKFL"
     gene            2051..4516
                     /locus_tag="PL056_10460"
     CDS             2051..4516
                     /locus_tag="PL056_10460"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPM47477.1"
                     /translation="MFVKRSEKPAIVTNSGTISYNQVIREINRFTLHLGDVTDERVLV
                     VSENRPEYIYAIYGIWRAGGTAVPVDFMSVPEEISYIIKDCRPSAVFISAGSLEKVKT
                     AIELSEIENVRIIVFEEVPPNGYSRTELDDIDFANHERTALLIYTSGTTGSPKGVMLS
                     FSNILANLEKLIEAGYYTSDEVVLMILPLHHIFPIMGTVIAVFYGGGATAIVPTLNSA
                     DIAHMMEISQPTMILGVPRFFDMLIKGIMAKINASFAARLLFKLAYKIDSVAFSKKIF
                     KSVALKFGGHLRHIISGGAPLNPETWNKFKALGFVIGEGYGMSEAAPMITFPRPGEGR
                     SGCVGKELERGTVKIVDGEVVATGKNIMKGYYNRPEETAEVIKDGWLYTGDLGRFDKD
                     GRLYITGRKKEIIVLPNGKNINPVEIEKDVEALNPVVAECAVIMKNGNLNAIIRPDEK
                     ILADSNIPNLEDYFRWEVIDKYNLNASHHKKILDFHLVSEPLPRTRLEKLQRFKLDSF
                     LEDKDKLKKSAEIPDDEIYSALAEYLKKATEKEIFPNDHVEIDLALDSLDKIELAGFI
                     SQKWKIRVSEEDLLKNPTVIKLSEFIKSENEKAASYSVDWKTQLKESISIDLPRSAFY
                     HWWIKFYLTTILRFFFRIRYYGYKFIPESPVIFAANHQSFIDGLFVVAKIKPSQFRRT
                     YFFAKEKHFKRKWRKYLANRNNIIIMKDGENVIDSIKRMASVLKKGRNIIIFPEGTRS
                     RDGQLGEFKQTFALLSKEMNVPVVPVAIHGAYDAFPRGRKIPHPFKKIVVEYLPAVDP
                     DGLTQEEITETVKNAIARVVEKK"
     gene            4772..>5330
                     /locus_tag="PL056_10465"
     CDS             4772..>5330
                     /locus_tag="PL056_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47478.1"
                     /translation="MIRVSVFVTCIFLFFNIYAEKIVVWNLKPQTGVTEKETVTVTSI
                     LTSEIERVSKKKVVSEAEMGSVIDGETMRIACGADDNVCIAEIGAAMGAPLSVSGILS
                     KMGDYWVINLQLVDIKKVETISRVNERFKGDMNSLVESLTPLVYKLFGKELPVKYDPE
                     KQQAIPEKKQKVKKERKHPSWQKATG"
BASE COUNT         1676 a          974 c         1181 g         1499 t
ORIGIN      
        1 atttgtaaat aataagaaaa tgaagatttc cagttatttt tgactctgaa tctcttgggg
       61 ttataattcc acagtttttt tgtctggtga acaattatgg cttttattaa acttctaaga
      121 ccgcatcaat atattaagaa tctactggtg ttctttcctc tgttcttcgc cttgaaaata
      181 aacaatattg cactgctgaa acagtcgtta attgctttcg ccgcattctg ttttgccgca
      241 agcaccattt atattgtcaa tgatatcaga gatatagaaa aagacagagc acatcctgaa
      301 aaaaagaacc gaccccttgc aagcggcaaa gtttcagtga aaaatgcaat cgttctgtct
      361 gcgctacttt ttcttgcggc tgttgcggtt tcttattttg gtggattaaa tcttttcctg
      421 atcatcggtt tttatattct gatgaacatc ggatattctt tcggactcaa aaatgttccg
      481 cttattgata tttttatagt ttcaacaggt tttcttcttc gtcttcttgc cggaacccat
      541 ttcggaggga tcgaagggat caacccttcc cactggataa taatcatgac atttctgatg
      601 agtctgttcc tcgcttttgc aaaaaggaga gacgaccttg tgcttgctga aaaaggggtc
      661 gaggtgagaa aatcaatttc gggatatacc attgaattta ttaacaatgc tatgagcgtg
      721 atgagtgcgg tactgatcgt ttcatatctt ctttacacac ttgatgcaaa agttcagaac
      781 catttccaga ctgaaaatct ttatgccacg actctttttg tcattttagg agtttttaga
      841 tatatgcagc tgacttttgt atttcataaa agcggaagcc cgacagacat tgtttacaag
      901 gacaggtttc tgcagcttac aattctcgga tggcttttaa tgttcctata ttttgcttac
      961 tgacggagga aaaagatgaa agttcttata acaggaggag ccggtttttt cggtcttcac
     1021 atgacaaaaa aatgtgtcga catgggagaa gaagtgactc tccttgatat tgctgaatat
     1081 gacaaagctg aatatgacaa atcggttaaa ctggtcaaag gcgatgtccg tgacgccgag
     1141 ataatggata agctgatatc tgaaaacgat gttgtgatcc atgctgcggc ggcacttcct
     1201 cttgaaaaaa agaaagagat attttcgaca aatgttgacg gaacaagaaa tgtgcttgaa
     1261 ctctgtttta agcataaaac cagaaaattt gtgatgatct cttcaactgc ggtttacgga
     1321 gttcccgacc atcatcctct ttttgaaact gacaaaatga tcggtgtcgg cccttacggt
     1381 gagtctaaga tcgcggctga gaaaatatgc agtgaatata gggacaaagg gcttttcata
     1441 acgacagtca gaccgaaaac cttcatcgga acccaccggc tcggcgtttt ccagatcctt
     1501 tacaactggg ttgaggaagg gcacagaata ccgatgatcg ggaacggaaa gaacagatat
     1561 cagcttcttg aagttgatga tctctgtgat gcggtttatc ttgcatatac ttctcaaaat
     1621 gaattggcaa acggagtttt caacatcggt gcagtgagca atttaagtgc aaacgactat
     1681 ctcggagcac tttgtgaata tgcaggaagc ggttcaacaa tactccacac cccggtttct
     1741 cttgttattc ctgcattgat gctctttgaa tggctccaca tctcaccgct ttacaaatgg
     1801 gtttacggaa cagctcataa agacagcttt gtttcagttg acaaagcacg cgaagtgctc
     1861 ggctggaacc ccaagtatga tgaatctcag gcgttgatca gaagctatca gtggtatcag
     1921 gacaacaagg catacatcgc aaaagggaca ggaacaagcc acagagttcc atgggcacag
     1981 gggattctgg gaataattaa aaagtttctg tgatctaatc tatcctatgt tgtgacaaaa
     2041 ggagaaaaaa ttgtttgtaa aaagaagtga aaagccggca atagtaacaa actcaggaac
     2101 aatttcatat aaccaggtca taagggagat aaaccgtttc acccttcacc tgggcgatgt
     2161 aactgatgaa agagttctgg tagtgagtga gaacagaccc gaatacattt atgccattta
     2221 cgggatttgg agagcgggcg ggacagcggt tcctgtcgat tttatgagtg ttcctgaaga
     2281 gatatcctac atcattaaag attgcagacc ttccgctgtt tttatatcgg cagggtcttt
     2341 ggaaaaagta aaaacggcga tagaactgtc agaaattgaa aatgtaagga tcatcgtttt
     2401 tgaagaggtg ccgccaaacg gttactcaag aactgaactt gatgacattg attttgccaa
     2461 ccacgaaaga acagcacttt tgatttatac ttcagggact acgggaagtc cgaaaggggt
     2521 catgctctct ttttcaaaca ttcttgcaaa tctcgaaaaa ctgatagaag ccggatacta
     2581 cacatctgat gaagttgttc tgatgattct tccgctgcac cacatttttc cgataatggg
     2641 gactgtcatt gctgtttttt acggcggcgg tgcaactgcg atagttccta ctctcaattc
     2701 agccgatatt gctcacatga tggagatctc tcaaccgaca atgatactcg gagtgccgag
     2761 attttttgac atgctgataa aagggataat ggctaagatc aatgcaagct ttgctgcccg
     2821 gctccttttt aagcttgctt ataagataga cagtgtcgcg ttttcaaaaa agatattcaa
     2881 atcggtggct ttgaaattcg gcggacatct ccgtcacata atcagtggcg gtgcaccgct
     2941 taatcctgaa acatggaata aattcaaggc attgggcttt gtgatcggtg aaggttacgg
     3001 aatgagcgaa gcagcaccca tgataacttt tccaagaccg ggggaaggga ggtcaggttg
     3061 tgtcggcaag gagcttgaga gagggacagt taaaatagtt gacggtgaag tcgttgcaac
     3121 cggaaagaac ataatgaagg gttactacaa caggcctgag gaaaccgcag aagtgataaa
     3181 ggacggctgg ctttacacag gagacctcgg aaggtttgat aaagacggtc ggctctatat
     3241 aaccgggcgg aaaaaagaga taatcgttct tccaaacggc aaaaacatca atcctgtcga
     3301 aatagaaaaa gatgtcgagg cactgaatcc tgtggttgct gaatgtgcag taataatgaa
     3361 gaacggaaat ctgaacgcaa taatcaggcc ggatgagaaa atacttgcgg acagcaacat
     3421 tcccaatctt gaggattatt tcagatggga agttatagat aagtacaatt taaatgccag
     3481 tcaccacaag aaaatacttg attttcacct tgtcagcgaa ccgcttccga gaacccgcct
     3541 tgaaaagctt cagagattca aacttgatag ctttcttgaa gataaggata aattgaaaaa
     3601 gagtgctgaa attcctgatg atgagattta ttcagcactt gcagaatatc tgaaaaaagc
     3661 gacagagaaa gagatttttc caaatgatca tgtcgagata gatcttgctc ttgattcact
     3721 tgataaaatt gaacttgccg gatttataag ccagaagtgg aaaataaggg tttctgaaga
     3781 agatctcctt aaaaatccga cagttataaa actttcagag ttcataaaaa gtgaaaatga
     3841 aaaagcggca tcctattctg tggactggaa gacacagctt aaggaaagca tcagcataga
     3901 tctgccgagg agcgctttct accactggtg gataaaattt tatctcacca cgattctccg
     3961 tttctttttc agaataagat actacggcta caagttcatt ccggaaagtc cggtcatttt
     4021 tgccgcaaat caccagagtt tcattgacgg actttttgtt gttgcaaaga ttaagccgtc
     4081 acagttccgc aggacatatt tctttgccaa agagaagcat ttcaaaagaa aatggagaaa
     4141 atatctggca aaccgcaaca acatcataat catgaaagac ggtgaaaatg tgatagattc
     4201 gataaaaagg atggcaagcg ttctcaaaaa agggcggaat atcataattt tccctgaagg
     4261 gacaagaagc agagatggtc aactcggtga gttcaaacaa acttttgccc ttttatccaa
     4321 ggagatgaat gtccctgtcg tgccggttgc gatccatggt gcgtatgatg catttccccg
     4381 cggcagaaag atccctcatc cttttaaaaa gatagttgtg gaatatcttc ccgccgttga
     4441 tccggatgga ttgactcagg aagaaataac cgaaactgtc aaaaatgcga tcgcaagggt
     4501 agttgaaaag aaataagaga attaattaat agaccgatct ttcgtaaagg gtgagcttaa
     4561 aaaaaatttc tttggcaaac ttgttcaata atttcagctg tatttaaagt ttacccattt
     4621 ttaagttcca cttcttttta agctaagtaa ttgtttttta aagactctct gttgttgcct
     4681 tagatttttt atgggaactt catgacctgg attaattgac aaaatagaaa aatgctaata
     4741 gagttttttt gacacaaagg gagatgaaaa aatgataagg gtttcggttt ttgtaacttg
     4801 tatatttctt ttttttaata tctacgctga gaagattgtt gtctggaatc tgaagccgca
     4861 gacaggggta acagaaaaag aaacggtgac agttacttct attcttacaa gtgagataga
     4921 gcgtgtttca aagaaaaaag ttgtgagtga agcagaaatg ggatccgtta ttgacggaga
     4981 aactatgcgc atcgcctgtg gagctgatga caatgtctgc atagcagaga taggagcggc
     5041 aatgggagct cctttatcgg tttccggaat attatcaaaa atgggagatt actgggtaat
     5101 taatttacag cttgttgaca ttaaaaaggt tgaaacgatt tcaagggtaa atgaaagatt
     5161 taaaggtgac atgaattccc ttgttgaatc tttaacacct cttgtctata aacttttcgg
     5221 gaaagaattg ccggtaaaat atgatcctga aaaacaacaa gctattcctg aaaaaaaaca
     5281 aaaagtaaaa aaagagagga aacacccatc atggcagaag gcgaccggct
//
LOCUS       DAOOYB010000197         5238 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_18354_length_5238_cov_14.934015, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000197 DAOOYB010000000
VERSION     DAOOYB010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5238
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18354_length_5238_cov_14.934015"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            87..1628
                     /locus_tag="PL056_10470"
     CDS             87..1628
                     /locus_tag="PL056_10470"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HPM47479.1"
                     /translation="MFKRFSLIAAIILVFMEVSAQYAALLDDKIKLNNEDFIKSLVEA
                     DNIVLGRYLQGGKTKAKIRVENAFVGEAKDEIIVNNLDNEKIRLRFKRDEYKKGDQYI
                     FILKKQGAEYDLLEYSVSIPVSDNLANFSFNTPYLINFWQPFDIKLFEVAAIAIREKE
                     DKMLSDNTRETFIELVKEYVGKNSVNDLKAALAIANLTEISLDFETYNKFASDTGTLG
                     CLAVKFSAGIMGEIYFNQNILPKAENMNPDSQTAFAVAAMKLSSKQGARVIGKILNNV
                     GLYRPSSSECFPYVKPPTNKEFFVRSVIEIDAPETLKILQLQIESGDAAWLASVLAII
                     SEYEGADLTELVLKAATVERLSERKYEFSNYFDKIKTPTTAKILTELFAKNEDLYWKK
                     IILTTIGKYQYAESLPFLIKTLNEDSKEEIRTTAAMSIGQLSQVGGAKPLYDFITKEK
                     SILAKSIAIDSLAQIPDRSVQDFLKEIIKNEQDPKIRESAVNAMEDNLFILRYGKKKN
                     DNSGR"
     gene            1609..2322
                     /locus_tag="PL056_10475"
     CDS             1609..2322
                     /locus_tag="PL056_10475"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012082064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HPM47480.1"
                     /translation="MTTADDKIGSWLIWQNSIQPFLYKEDFSKEILSVIAQKNRDVKL
                     EIINIDKDELKPVERIKREVSKCLNCRLSQTRKNVVFGEGRIDADIMFIGEGPGEDED
                     RTGRPFVGKAGQLLTKIIENGMKISREEVYIANIVKCRPPSNRDPLPDEAEKCIGFLK
                     RQIEIINPKVIIILGKVAAKYLLGIETTITKAREETYDYGSSKVFVTYHPSALLRNES
                     YKRPVWEDIKKVLKYLETV"
     gene            complement(2300..3409)
                     /locus_tag="PL056_10480"
     CDS             complement(2300..3409)
                     /locus_tag="PL056_10480"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47481.1"
                     /translation="MSSLKIEIVKVISNGCGLGWGDGKTFFVPFTIPGEIVEITAAVK
                     EKGHFKATDFEIATASKHRRKPICAHYTHCGGCSFQHISYPEQMNLKKEIFLELLKQN
                     GIQLDIEPVLYSAGETNLRTRAKVFVDDGVPAFRAYRSNELVKFQNCPLLNREFIKNI
                     FDDSKKRRGDVQYEFSKFSKDLMPPKESVRKIVNGKEIKILRNSFFQSSEEGAGILCS
                     LLEDEIKASTPETGYDLFCGSGLFSVFMAVAGVKTTGMEIVHESCRSYEENLGNTAQI
                     ERVDAYKIKKLEKRDIVVADPPRDGLGTELVDIVAESKAKTVVYISCEPSKFARDMKR
                     FVDCGYKLKNLDIVDLFPSTHHFEVFSVFKRFQDI"
     gene            complement(3406..3945)
                     /locus_tag="PL056_10485"
     CDS             complement(3406..3945)
                     /locus_tag="PL056_10485"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HPM47482.1"
                     /translation="MKIVSQKLALDAGIFKVFEVKLLNNKKELTHSVLRFTGTICVLP
                     VTEKGTIILERQYRTALDRYILEIPAGKIDEGEEPELCLHRELEEETGLRAIKFKKCF
                     EGFVTCGYSDEYMHYYAALTEKVPDEERNHFPDPDEEIELVEITVEKALEMIKEKEII
                     DSKTITLIYLYASGAVHWE"
     gene            4064..5233
                     /locus_tag="PL056_10490"
     CDS             4064..5233
                     /locus_tag="PL056_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPM47483.1"
                     /translation="MEKQMTVERISQIILDLNLINDLDSLLEKILTETRALTNADAGT
                     IYLKEGDDLVFKNFQNNTLIEADEKSFRTALYSNIKVPVNDNSLAGYCASRNEIVNIE
                     DVYDMPKYVPYSFNNKFDEDTFYTTKSVLSVPLHDRYKNVSGVLQIINKIGSDYVPVA
                     FESDDLSVVKFFAANASAAIEKARITRETILRMIKMAELRDPMETGSHVNRVGAYSVE
                     IYRQWAKKNSVDEHEIKTYTDNMRIAAMLHDVGKVAISDTILKKPGKLDEVEFAIIKT
                     HPDEGTKIFENRFYEVDEMAAVISLTHHEKFDGTGYPKGIAGKDIPLCGRIVAIADVY
                     DALVSKRVYKEAWPEEEVIREITSLSGKHFDPELVKCFVEIHEVIKAVRAKYPDK"
BASE COUNT         1696 a         1013 c         1015 g         1514 t
ORIGIN      
        1 tgttcattaa tattgacaaa gttgattttt atgtataaat ttatagcgga acaaaatttt
       61 tagctcttgc agcacggagg acagttatgt tcaaaaggtt ttctttgata gcggcgatca
      121 ttttggtatt catggaagtt tctgcgcagt atgccgcact tcttgacgac aagataaagc
      181 ttaataatga agatttcata aagtcccttg tcgaagcaga taacattgtt cttggaagat
      241 atcttcaggg cggaaagact aaagctaaaa tcagagttga aaatgctttc gtcggggaag
      301 ctaaagatga aataattgta aacaacctgg acaatgaaaa gatacgattg agattcaaaa
      361 gagacgaata caaaaaggga gatcagtata ttttcatcct caagaaacag ggagcggaat
      421 atgatctgct tgaatacagc gtttcaatac ccgtttcaga caaccttgca aatttctcgt
      481 tcaacacacc ctatctgata aatttctggc agccttttga cattaagctt tttgaagttg
      541 cagcaattgc gattagagaa aaagaagata aaatgctttc tgataacaca agagaaactt
      601 ttattgaact tgttaaagaa tatgtcggca aaaacagcgt aaacgatctc aaggcggctc
      661 tcgctattgc gaatttgact gaaatatctc ttgattttga aacttacaat aaatttgcat
      721 ctgacaccgg tacgctcgga tgtctcgcag tcaaattttc tgcaggaata atgggagaga
      781 tatatttcaa ccagaatatc cttccgaaag ctgaaaacat gaatcctgac agtcagacgg
      841 cttttgcagt tgctgcaatg aagctttctt caaaacaggg agcaagggtt atcggaaaga
      901 tcctgaacaa tgtgggattg tatcgccctt cttcaagtga gtgcttcccc tatgtgaaac
      961 cacctacaaa caaggagttt tttgtaagat cagtaataga aattgatgct cctgaaacat
     1021 tgaagatcct tcaactccag atagaaagcg gtgatgcggc atggcttgcc tctgttcttg
     1081 ctatcatttc tgaatacgaa ggtgcagatc ttaccgaact tgttctgaaa gcggcaactg
     1141 tcgaacggct ttcagagaga aaatatgaat tctcaaatta ttttgacaaa ataaaaaccc
     1201 ctacaacagc taaaatactg acagaacttt ttgctaagaa tgaagacctt tactggaaaa
     1261 aaataatcct tacaaccata ggaaaatatc agtatgcgga atctctcccc ttccttatca
     1321 aaacgctcaa tgaagactcg aaagaggaaa taaggaccac agcagcaatg tcaatcggtc
     1381 agctcagtca ggtcggagga gcgaaacctc tttatgattt cataacaaaa gaaaaatcta
     1441 tccttgcaaa atctatcgcc attgattcgc ttgcacagat ccctgacaga tctgtacagg
     1501 actttttaaa agagatcatt aaaaacgaac aggacccgaa aatcagagaa tcagctgtaa
     1561 atgcaatgga agataatctt ttcattttaa gatacggcaa aaagaaaaat gacaacagcg
     1621 gacgataaga tcggttcctg gctcatctgg cagaattcca tccagccgtt tctctataaa
     1681 gaagattttt caaaagagat cttgtcagta attgctcaaa agaatagaga tgtaaaacta
     1741 gagatcataa atattgataa agatgaattg aaaccagtcg aaaggataaa aagggaggtt
     1801 tccaaatgtc ttaactgccg tcttagtcag acaaggaaaa atgttgtttt cggtgaaggc
     1861 aggattgacg cagatattat gttcatcggt gaaggacccg gtgaagatga ggacagaacg
     1921 ggaagacctt ttgtcggaaa agcaggacag ctcctcacaa aaattatcga aaacggaatg
     1981 aaaatatcgc gggaagaagt ctatatcgca aatatcgtaa aatgcaggcc tccgtcaaac
     2041 agggatcctc ttcccgatga agctgagaaa tgcatcggat ttctgaaaag gcagatagag
     2101 ataataaacc cgaaagtgat aataattctt ggaaaagttg cggcaaaata tcttctcggg
     2161 attgaaacaa cgataacaaa agcccgcgag gaaacttacg attacggttc ttcaaaagtt
     2221 tttgtgacat accatccgag tgcactttta agaaacgaaa gttataaaag acccgtctgg
     2281 gaagatatca aaaaagttct taaatatctt gaaaccgttt aaagactgaa aaaacttcaa
     2341 aatggtgtgt tgaaggaaaa agatccacaa tatcaagatt ttttaattta tatccgcaat
     2401 caacaaatct tttcatgtca cgagcgaact ttgagggttc gcaggagata taaacaactg
     2461 ttttcgcttt actttctgca acaatgtcaa caagttcagt accaagacca tctcttggcg
     2521 gatcggctac aacgatatct ctcttttcaa gtttctttat tttataggca tcaactcttt
     2581 caatttgagc agtattaccg agattctctt cataacttct gcatgattcg tgaacaattt
     2641 ccattcctgt cgttttcact cctgcgacag ccataaaaac agaaaaaagt ccgcttccgc
     2701 aaaatagatc atagccagtt tcaggtgttg atgcttttat ctcatcttca agcagagaac
     2761 ataaaattcc ggcaccttcc tctgagcttt gaaaaaaact gttccttaag atctttatct
     2821 cttttccgtt cacaattttt ctgacacttt cttttggcgg catgagatct tttgagaatt
     2881 ttgagaattc atattgaaca tcgcctcttc ttttttttga atcatcaaaa atatttttaa
     2941 taaattcccg gttcaggaga ggacaattct gaaattttac aagttcattt gatctgtatg
     3001 ccctgaaagc gggaactccg tcatcaacaa aaactttggc tcttgttctt agattagtct
     3061 ctcctgcaga atagagcacc ggctcaatat caagttggat cccgttctgc tttaaaagtt
     3121 caagaaagat ctcttttttc aagttcatct gttcaggata gctgatgtgc tgaaatgagc
     3181 atccgccaca atgagtatag tgggcacata ttggctttct tctgtgtttt gaagcagttg
     3241 cgatctcaaa atctgttgct ttgaaatgtc ctttttcttt gactgcggct gttatttcaa
     3301 caatctctcc gggtattgtg aatggcacaa aaaatgtttt tccatcgccc catccaagcc
     3361 cgcaaccgtt agagatcact tttactattt caatctttaa agaactcatt cccagtgtac
     3421 cgctcctgac gcataaagat atatcaaagt gatcgttttt gaatctatta tctctttttc
     3481 ttttatcatt tcaagagctt tttccacagt tatttcaaca agttcgatct cttcatccgg
     3541 gtcagggaaa tggttccttt cttcatcagg aactttttct gtaagtgccg cataataatg
     3601 catatattca tcgctgtatc cgcaggtgac aaacccttca aaacattttt taaactttat
     3661 agctctaagt cctgtctctt cttcaagttc gcggtgaagg cacaattccg gttcttctcc
     3721 ttcatctatt tttcctgccg ggatttcgag aatatatcgg tcaagagctg ttctatactg
     3781 ccgttcaaga attatcgtcc ctttttctgt cactggaaga acacatatcg ttccggtgaa
     3841 gcgaaggaca gaatgggtca actccttctt attattaagc agcttaactt caaaaacttt
     3901 aaatattccg gcgtccagag ccagcttctg agatacaatt ttcataactt cctccaaaaa
     3961 gatcagctca aattatcaga atcgcaccac aatacaaatt ttttgctttc ataagctgtt
     4021 tctgatattt tctcacgatt tttataactt atcggaggaa aacatggaaa agcagatgac
     4081 agttgagaga atttcgcaga taattcttga tcttaatctc attaacgatc tggattcgct
     4141 tcttgaaaag atactgactg aaacaagagc acttactaat gctgatgcgg gaacgatcta
     4201 tcttaaagag ggtgacgatc tcgttttcaa gaactttcag aacaacactc tgatcgaagc
     4261 agatgaaaaa agcttcagga cagcccttta ttccaacata aaagttccgg tgaacgacaa
     4321 ttctcttgcc ggatactgcg cctcacggaa tgaaatagta aatattgaag atgtttacga
     4381 tatgcctaaa tatgttcctt acagtttcaa caacaaattt gatgaagata ctttctatac
     4441 gacaaaatct gtcctttcag ttccattaca cgacagatac aagaatgttt ccggggtgtt
     4501 acagataata aacaaaattg gttccgatta tgttcccgtg gcatttgaaa gcgacgatct
     4561 ttctgtggtc aaattttttg cggcaaacgc ttcggcggcg atcgaaaaag caagaataac
     4621 aagagaaacg attctcagaa tgataaaaat ggctgaactt cgtgatccga tggagacagg
     4681 cagtcatgtc aacagggtcg gagcatacag tgttgaaata tatcgccagt gggcaaaaaa
     4741 gaacagcgtt gatgaacatg agataaaaac atataccgac aacatgagga tagccgcaat
     4801 gcttcatgat gtaggcaagg tggcgatttc agacactatt ttgaaaaagc cggggaaact
     4861 tgatgaagtt gagtttgcga taataaagac acatcctgac gaaggaacaa aaatatttga
     4921 gaaccgtttt tatgaagttg atgaaatggc ggcggtaatt tctttgaccc atcatgaaaa
     4981 atttgacggg acaggttatc ccaaaggtat tgcaggaaaa gacattcctc tttgcggaag
     5041 gatagttgcg atcgctgatg tttatgatgc actcgtttca aaaagggttt ataaggaagc
     5101 gtggcccgaa gaagaagtca tcagggaaat aacttcactc tcaggaaagc attttgaccc
     5161 tgaacttgtt aaatgtttcg tcgaaatcca cgaagtcatc aaagccgtcc gggcaaaata
     5221 tccggataag tgacatct
//
LOCUS       DAOOYB010000198         5226 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_18404_length_5226_cov_10.740089, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000198 DAOOYB010000000
VERSION     DAOOYB010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5226
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18404_length_5226_cov_10.740089"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2828)
                     /locus_tag="PL056_10495"
     CDS             complement(<1..2828)
                     /locus_tag="PL056_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002671028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HPM47484.1"
                     /translation="MKKVLFIAFTLFCFFNLFGAVNALGPTQCGVDDDGKMCDLEVNR
                     KKVCLNLSCVEKGAYGFFAETGSAAENTQWSNKYFIDHNMLLDNGRPVVPVFVVNLNC
                     SDILAPAVNADNFEYTVLFAEQHFKEASFNQFAPVFRVYNYDRDGSIPDVFPTTRSYT
                     CLSPSNDPADPDYIHPYNGGPNVYEIFKHCRPNVGAGGIEDGNINCSTPEWKTEMHSG
                     SFQEMFLIRMFEDHIQTIGNLENISALKYTATADGVPIYYIPVTGLLYSQYGGGQAQQ
                     DLSFDVTLEDNNGNDKTVRIITSVGNTTYYNDIKDLNGNIKDLNGRPWTFVHELSHGR
                     LLSDFYSDDTAVGFSSTFSIMSYTHTEGDSGGLDFLLFHKFMLGWIRNDNQNEFLKRI
                     EVSHNQNSGKIEIKENGNLRNNECADIKGSVCITFTHVLPTTNAYISQRILNPAEDVI
                     MPMYQKHFMIYTDYDPMDLLKFPTPVELKPDSVIMGRILHTGLEATASGAYAVGDAVR
                     ERFPSTQNYLSGVLLYRYNRDGRTRIDFTNPSPEFLPYFDIIEQNGIDTKSTFYLRAV
                     ELYEKVTGVSLPIGTYFDDDINRVNWINNRINPGVSDWIKGLYPYVWDTLSNNWIVDV
                     DSELVNDPTIWEKIVLASREDIDRNEPWVSFSGWPGNYFRFNTNDYLNSVSERRKRMV
                     DANTGDLVGSLPNENIVIETLSHKKIAVSIFNMRKTNTGNSVNYSYKLVFTDLDFPVG
                     KADSYISYDKDEDGFCDMQCIGTIPGFCLSDFDETNIDTTMGSKVRNTWINAGYNQTN
                     ICLPVDNCPTVHNPDQLDTDGDGVGDACDNCPNISNSDQLDSDNDGVGDVCDTCPGIY
                     NPVVDYSLDSEFISGFEGAIARDLCRESKKWVFIGGQPFFVLRDVCSMQPDSDLDGIG
                     DACDFTSAGGNGFANSRITNAKPKTIIPS"
     gene            3092..>5226
                     /locus_tag="PL056_10500"
     CDS             3092..>5226
                     /locus_tag="PL056_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47485.1"
                     /translation="MNLFTKIFFIIFLSFSILISCDNQKAGTFAVKFSWAKDDAGKEI
                     KPDVSTGEFFVTVRIYEWKEGVAFPGDIAANGKQLIQSDPAQMKVKGTSIDFGDLSYG
                     KRRFVVAEIRKGAELTNSVLFTGMSQLFELKAGKHTEVNVEMSLTGLPGYDENGDNTF
                     KIWIFHKDSPADRVPESRVALRFTVINANSVIIANDLNFEKGTAEIALTDLKKIDETT
                     YEYSPWDFTTGWEEIGDGTYTVFGKLKNNIGQIGEPKRADVFLDTTPPALNITPFKEV
                     AKLGDRIEVRFLFDEAVDPEALVLDWQGLPFALEENKEDKSFVYTYTVQEEDNEIEYQ
                     FSLTAADVVGNKTDVVDFGKVRIDTTPPDLNYIAMFKNGTEHGLSSNLYLKHGDILRF
                     ELELNEEITEIPQLLLGPHKIICEEGEKELSFDCSLTIDKDVTYDVVADISLSFNDIA
                     GNIYSRSIVSGAKIDTQVPQFIFRKNKDPQDYNATDTIVLTITANEPVSMIEVNGTEL
                     DNTLTSWNISGKNETGTHEVKLKAVDLAGNEQTEFTVGTYKVDADYPSAVISNPAPVR
                     ISGSGSSVISVTEWTKPIQSIKLNGVECDGAYCTFNAPSGSGDSIESLTVTLTDFAGN
                     ISNISAGTVYVDRTAPEVSGDATVIFTKPARCPLSSVSSITSGSSVDISFIVNEPLQE
                     LGDPLVSAHGDVNIIDFNITGQIGYFYTF"
BASE COUNT         1686 a         1021 c          978 g         1541 t
ORIGIN      
        1 gatggaatta ttgttttcgg cttggcattg gtgatcctgc tgtttgcaaa accgttgccg
       61 cctgctgaag taaagtcaca ggcatcacca atcccgtcaa ggtcgctgtc aggctgcata
      121 gaacagacat ctcttaaaac aaaaaacggt tgtccgccaa tgaacaccca ttttttgctt
      181 tcccgacaca aatctcttgc aatagcacct tcaaatcctg aaataaattc cgaatcaaga
      241 ctgtaatcaa caaccggatt ataaattccg gggcaggtgt cacacacatc accgacaccg
      301 tcattatccg aatcgagttg atctgaattg gaaatattag gacaattatc gcatgcatcg
      361 ccgacaccgt caccgtcagt atcgagctga tcgggattat gaacagttgg gcagttgtct
      421 acgggaagac aaatgttggt ttgattatat cccgcattta tccatgtatt gcgaaccttt
      481 gaacccattg tcgtatcaat attggtttca tcaaaatcgg acaaacaaaa tccaggaatt
      541 gttccaatgc actgcatatc acaaaaaccg tcttcgtctt tatcatatga aatatatgaa
      601 tctgcttttc ccactgggaa gtccaaatcg gtaaaaacta atttatatga gtaattaact
      661 gaattcccgg tattggtttt tctcatgtta aaaattgata ccgctatttt tttatgagac
      721 aatgtttcta ttacaatatt ctcgtttgga agagagccga ctaaatcacc agtgtttgca
      781 tctaccattc tttttcttct ttcactaaca ctgttcagat aatcgttggt attaaatcta
      841 aaataattgc ccggccatcc agaaaaacta acccacggct cgtttctatc tatatcctct
      901 ctgctggcca atacaatttt ttcccatata gtcggatcat ttacaagctc gctgtcaaca
      961 tcaacaatcc aattattgga caatgtgtcc cagacatatg gatacagtcc ttttatccag
     1021 tcagacaccc caggattgat tctgttgtta atccaattaa ctctgtttat atcatcatca
     1081 aaataagtgc cgataggcaa agaaacacca gtaacttttt catataattc aaccgctcga
     1141 agataaaaag ttgatttagt gtctattccg ttttgttcaa taatatcaaa atatggaaga
     1201 aattcagggc ttggatttgt aaaatctatg cgtgttcttc cgtctctgtt atacctatat
     1261 aataaaactc cggacaaata attctgggtt gatggaaatc tttctctaac cgcatctcct
     1321 actgcgtaag cacctgatgc tgtggcttca agtccggtgt gaagaattct acccattatt
     1381 acagagtccg gtttcagttc taccggtgta ggaaatttta acaaatccat ggggtcataa
     1441 tcagtataaa tcataaaatg tttttgatac atgggcatta taacatcttc tgccggattc
     1501 agaatccttt gagaaatgta tgcattggtg gtcggtaaaa catgggtgaa ggttatgcaa
     1561 accgatcctt ttatatcagc acattcatta tttctcaaat tcccattttc ttttatctct
     1621 atttttccac tgttctggtt atgcgacacc tctattcttt ttagaaattc gttttgatta
     1681 tcgtttctta tccatcccag cataaattta tgaaacaata aaaaatccag tcctccagaa
     1741 tctccttctg tgtgtgtata tgacataatt gaaaatgtag acgaaaatcc aacagcggta
     1801 tcatcactat aaaaatctga caatagccgt ccatgagaca attcatgtac aaaagtccat
     1861 ggtctaccat taaggtcttt tatgttacca ttaaggtctt ttatatcatt ataatatgtt
     1921 gtgtttccga ctgatgttat aatcctaacc gttttatcat ttccattatt atcttcaagt
     1981 gtcacatcaa aagacaagtc ctgttgagcc tgccctccac catactgaga ataaagtaga
     2041 cctgttacag gtatatagta tataggaact ccatcagcag tagcggtata ctttaaagca
     2101 gaaatatttt caagattacc aattgtttga atatgatctt caaacattct aattaaaaac
     2161 atctcttgaa aagatcctga atgcatttct gttttccact caggggtaga acagttaata
     2221 ttgccatctt caataccacc agctccaaca tttggtcggc agtgtttaaa tatttcatag
     2281 acattggggc cgccattata tggatggata taatcaggat cagcaggatc attagaagga
     2341 gaaaggcagg tatagcttct tgttgttgga aaaacatctg ggatcgagcc atctctatca
     2401 taattataaa ccctaaaaac aggagcaaac tgattaaaag aagcctcttt aaaatgctgt
     2461 tcagcgaata aaacagtata ttcaaaatta tcagcattga cagcgggagc taaaatatcg
     2521 gaacaattaa gattgacaac aaaaaccgga accacaggtc tgccattatc caacaacatg
     2581 ttatgatcta taaaatattt gtttgaccat tgggtgtttt ctgctgcact tccagtttct
     2641 gcaaaaaagc catatgctcc cttttcgaca caacttaaat ttaagcaaac tttttttcta
     2701 ttcacctcaa gatcacacat ttttccatca tcgtctacac cacattgcgt aggacctaaa
     2761 gcattgactg ctccaaaaag attgaaaaaa caaaataatg taaaagcaat aaacaatacc
     2821 ttcttcatca cacgccccca aaaaaaaatt ataaaaaacc tcaccttaaa agtcctgaat
     2881 cagaagaagt tcctgtcttg tcagcatcag agtggactta gtcttataaa aaacatgctc
     2941 actccgatgc ataatttata atctccttca aattcaaaac attgtaactt aaagatttaa
     3001 aacagcaaaa tttcctgaca cataatttct ttaaaaaata aaaaaaacag atagtattaa
     3061 caagcaaaac aattctttgg agtgacatac aatgaattta tttacaaaga tttttttcat
     3121 tatctttcta tctttttcaa ttctcatctc ctgcgacaat cagaaagcgg gaacctttgc
     3181 agtgaagttc tcatgggcta aagatgatgc agggaaagag ataaagccgg atgtttcaac
     3241 tggagagttt tttgtaaccg tccgaattta tgaatggaag gaaggggttg cattccctgg
     3301 tgacattgcg gcaaacggga aacagcttat tcagagtgat cctgcacaga tgaaagttaa
     3361 aggaacgagc atagatttcg gcgatctaag ttacggcaaa agaaggtttg tcgttgctga
     3421 gatcaggaaa ggtgcagaac tcacaaattc agttcttttt accgggatgt cacaactctt
     3481 tgaactcaaa gcaggaaaac acactgaagt aaatgttgaa atgtcgctga cagggttgcc
     3541 gggatatgat gagaacggag acaacacttt taaaatttgg atctttcaca aggattctcc
     3601 tgctgacaga gtgcctgagt caagagttgc attaagattt acggttataa atgccaacag
     3661 tgttattatt gcaaatgacc ttaattttga aaagggaacg gcggaaatag cacttaccga
     3721 cctgaaaaaa atagacgaaa cgacttatga atattcacca tgggatttca ctaccggatg
     3781 ggaggaaata ggagatggaa cttatacggt gtttggcaaa ttaaagaaca atatcggaca
     3841 gataggtgaa ccgaaaagag ccgatgtgtt tcttgacaca actccgcctg ctttgaatat
     3901 tacaccgttt aaggaagttg caaagctcgg tgacaggatc gaagtccgct ttttatttga
     3961 tgaagccgtt gatcctgaag cacttgtcct tgactggcag ggacttccat ttgcacttga
     4021 agaaaataag gaggataaaa gttttgttta cacttatact gttcaggagg aagataatga
     4081 aattgagtac cagttttcgc tgactgcggc cgatgttgtc ggaaataaaa ctgatgttgt
     4141 cgattttgga aaagtgagaa ttgacacaac tccacctgat cttaattaca ttgcaatgtt
     4201 taaaaatgga accgaacacg gtttgagctc aaatctttat ctaaagcacg gagatatctt
     4261 aaggtttgaa ctcgaattga atgaagagat cactgaaatt ccgcaacttt tgcttggacc
     4321 gcacaagatc atttgtgaag aaggggaaaa agagctttct tttgactgtt cactgacaat
     4381 tgataaagat gtgacttatg atgttgttgc cgacatatcg ctttctttta atgatattgc
     4441 gggcaacatt tacagcaggt cgatagtttc aggagcaaag atagatactc aagttccgca
     4501 gtttattttc agaaagaaca aagatccgca ggactacaat gcaacagaca caatagttct
     4561 gacgatcacg gcaaatgagc cggtttcaat gatagaagtt aacggaactg aacttgataa
     4621 cactcttaca agctggaata tttcaggtaa aaatgaaaca gggactcatg aagtaaaact
     4681 caaagcagtt gaccttgcag gaaatgagca gacagagttc acagtaggta catacaaagt
     4741 tgacgctgat tatccaagtg cggttatttc taatcctgca cctgtaagaa tatcgggaag
     4801 cgggtcatct gtaatttcag tgacagaatg gacaaaaccc attcagagca taaagcttaa
     4861 cggagttgaa tgtgacggag catactgcac atttaatgca ccttccggaa gtggtgacag
     4921 catagagagt ttgactgtga ctctcaccga ttttgcagga aacatctcaa acataagtgc
     4981 aggaactgtt tatgtggata gaactgcacc tgaagtctca ggtgatgcga cagtaatttt
     5041 tacaaaaccg gcaaggtgtc cgctcagttc agtaagcagc ataacttcgg gcagcagtgt
     5101 tgacatctct ttcattgtga acgaacccct gcaggaacta ggggatccgc ttgtaagtgc
     5161 tcatggagat gtaaacatta ttgattttaa cattaccgga cagatcggat atttctatac
     5221 attcag
//
LOCUS       DAOOYB010000199         5206 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_18492_length_5206_cov_9.824112, whole genome shotgun sequence.
ACCESSION   DAOOYB010000199 DAOOYB010000000
VERSION     DAOOYB010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5206
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18492_length_5206_cov_9.824112"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="PL056_10505"
     CDS             complement(<1..230)
                     /locus_tag="PL056_10505"
                     /inference="COORDINATES: protein motif:HMM:NF019924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HPM47486.1"
                     /translation="MKVLIAASELQPFASSGALAETLSCLISSISGHVDIEYVMPLYS
                     MIDESIYDIKPTGKGFQVPVADKIENAVVWVG"
     gene            434..2086
                     /locus_tag="PL056_10510"
     CDS             434..2086
                     /locus_tag="PL056_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylase"
                     /protein_id="HPM47487.1"
                     /translation="MVKKLLVRDLTLRDGQQSQFATRMSQKQVDKILKMYKNAHFYAM
                     EVWGGAVPDSIMRYLNEDPWDRLEKIKEGIGSSSKLTALSRGRNLFGYNPYPEDVIEG
                     FNRNAIKSGIDIMRIFDALNDLDNMRSTIKYVKENGGLADCCVCYTVDPKFSFRDRIK
                     AFFSGKKLPSNLFSVDYFVKKSIELEKMGADMISIKDMAGLIDPETSSMLISALKKNV
                     SIPIDLHTHCTPGYGLGSVLMAIIKGADIVDTVIMPFAGGPAAPAFELVQIFATKLGI
                     DTGVNLEAVREIRKELFEMRKELCGFDQYKMFPLDFDPAKDKLPPEIDKMFDDAIAAA
                     KKDDTDAVTSLTQAIEKYFNFPAPDDIVRIAQIPGGMYTNMLSQLQAANLGHLLEKVL
                     KTVPVVRLDAGVPPLVTPTSQIVGIQAVSCVINEEQRKGFYDNTSIQFVNLVKGIYGH
                     TPWPVDPDFRKKIAGHKEETPYDTSKHVKQTNEILREFGGRKLASDEKEELLLELFPA
                     VAKPFLKGKRELEFLNEIREIQERTDKERGVLMTALEENFLM"
     gene            2093..3421
                     /locus_tag="PL056_10515"
     CDS             2093..3421
                     /locus_tag="PL056_10515"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HPM47488.1"
                     /translation="MNENLNQYINSEKKLKIRIEKLLSTLHLSKFSGTVNVINGDDRF
                     NIIVTEGKSYCERSLLQISTLPSGTLFMLKTELPEGFKVNLKEYDSLDFIFRIVENIE
                     RSIIVAMIKPVATEHFSIRPEASSMTFLHDELESFIGQKRIEIFSSFKKHYRTIFFLL
                     LTRFASVVRSTDEERANIQEMLSRSNMFDEKATEEETAEEVIEQSEDQKLVSFLDINE
                     KFKDVYHLFGLSFDFDEKALHNTYLKIVQKIHPDALRGVNVVNRDRAEKFFRVVSENY
                     EMLRNPEERADVEKLMKRYGPIKSRTDLTRMKEYDEAIFKGTALLRLGNFEGAAEIFG
                     MIYDQTKNPDALEKKILAIWKKSPRWNDFEKTIKYPPIKEDILKLTMIKDPDIEILFI
                     LAEIYEFLKMIPDCMKVLTIITKIQPDNFRAKGMKKRLMYYQSLEKQGKK"
     gene            3418..4020
                     /locus_tag="PL056_10520"
     CDS             3418..4020
                     /locus_tag="PL056_10520"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HPM47489.1"
                     /translation="MSIFIKASVFFLMMTSLLYSSVSIYYFYDPIKDRYTFTNQCSDL
                     RSCKPLYQSKGEVKHKRGYALKPGKESAFDDIIQKASVKHSVDFHLIKSVIKAESLFD
                     TKAVSSAGAKGLMQLMPATAVEVGVKDVFDAQQNIMGGTLYLKKMIKRFKNTRSALAG
                     YNAGPAAVVYYNGIPPFNETVAYVEKVSDFYKNYTGRELW"
     gene            4014..4667
                     /gene="pgsA"
                     /locus_tag="PL056_10525"
     CDS             4014..4667
                     /gene="pgsA"
                     /locus_tag="PL056_10525"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HPM47490.1"
                     /translation="MVKRFRKNKKSTGDFGRRKVIMDKLINFKKDVLTIPNLLTGIRI
                     FFVPFVLFLIWYDTPVSRLWSWIFYTIATVTDFFDGYIARKHGTVTVTGKLLDPLADK
                     FIVNLTLVLLVSMGEMHVLPVLIILAREFYIFGIRSVAVERGVVVAAGQGGKMKTMFQ
                     MFALPFFMINQNTLIGLFNIDWDPRLWGYILIWGSIFFSVTSAYTYSKEVKRKLFRS"
     gene            complement(4664..>5206)
                     /locus_tag="PL056_10530"
     CDS             complement(4664..>5206)
                     /locus_tag="PL056_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPM47491.1"
                     /translation="PQKSDISVMSPSARDKSMAPDGKGTLTIYCPAYYSDFSTLSKDE
                     YVKFKNDFAHILINRVEKALSENIRSHIEFMDVATPVTYHRYTGNREGVMMGARPGKV
                     NFKARIAHFVTPLENVFLCGHWSALGGGVPIAVSTAANVALMVLKNDGNRFFKPLGKY
                     FDGSVSVQEFNKCLARSENS"
BASE COUNT         1640 a         1045 c         1095 g         1426 t
ORIGIN      
        1 cttccaaccc agactactgc attttcgatc ttatctgcaa caggcacctg gaatcccttc
       61 cctgtcggtt tgatatcgta tatgctttca tcgatcatac tgtaaagagg catcacatat
      121 tcaatatcga catgacctga aatgctggat atcagacagc tcaatgtttc agcaagagct
      181 ccggatgaag caaacggttg cagctctgaa gcggctatta ggactttcat ttttacacct
      241 gaaataaaca aacatcaaaa cactcttttt ccgatggaat gataacgaac cgtcttaaac
      301 agtcaatttt ttatatattc aaatatttta gcagtatttt tataaatatt attgaattaa
      361 tgattgttat tgataaaatg ccccggtgtg atagctcttg atagttcgaa aattaaggat
      421 atagaggttg aaaatggtta aaaaactcct tgtgagagat ctcactcttc gtgatggaca
      481 acagtcccag tttgcaacaa gaatgtctca aaaacaggtt gataagattc tgaaaatgta
      541 taaaaacgcc catttttacg caatggaggt ctggggtgga gcagttccgg attcaattat
      601 gagatatctg aacgaggatc catgggaccg gcttgaaaaa ataaaggaag gtatcggatc
      661 atccagcaag ctcacggctc tctcaagggg aagaaacctt ttcggataca acccttatcc
      721 tgaagatgtt attgaaggtt tcaatcgtaa tgccattaag tccggcattg acataatgag
      781 gatctttgat gctctcaacg accttgacaa catgaggtcg accataaagt atgttaagga
      841 gaacggaggt cttgcggact gctgtgtctg ttatactgta gatccgaaat tctcattcag
      901 ggacagaatt aaagcgtttt tcagtggtaa aaaacttccg tcaaaccttt tttccgttga
      961 ttactttgtg aaaaagtcca tcgagcttga aaaaatgggc gccgacatga taagcataaa
     1021 ggatatggca ggacttatag atccggaaac atcatccatg ctcatttccg ctcttaaaaa
     1081 gaatgtttcg atccctattg accttcatac acactgcact ccgggatacg gtctcggaag
     1141 tgtactgatg gctataataa agggcgctga tattgttgac acagtaatta tgccctttgc
     1201 tggcgggccg gctgctccgg cttttgaact tgtccagata tttgcaacaa agctcggaat
     1261 tgatacaggg gtcaaccttg aggcagtcag agaaataaga aaagagcttt tcgaaatgag
     1321 aaaggagctt tgcggttttg atcagtacaa gatgttcccg cttgatttcg atcctgcaaa
     1381 agacaaactt ccacccgaaa ttgacaaaat gttcgatgat gcgatagcgg cagcaaaaaa
     1441 agatgatact gacgccgtca cctctttgac acaggcgata gaaaaatatt tcaacttccc
     1501 cgctcccgac gatatcgtaa ggatcgctca gatcccggga ggaatgtaca ccaacatgct
     1561 ttcacagctt caggcggcaa atctggggca tctgcttgaa aaagttctca agaccgttcc
     1621 tgttgtccgt cttgatgccg gagttcctcc tcttgtaact ccgacaagtc agatcgtggg
     1681 tatacaggca gtaagctgtg tcataaacga agaacagcgg aaaggatttt atgacaacac
     1741 ttcgattcag ttcgtaaatc ttgtgaaagg catctacggt catactccgt ggcctgttga
     1801 tcccgatttc aggaagaaga tcgccggaca taaagaggaa accccttacg acacatcaaa
     1861 acatgtaaag cagaccaatg aaatactcag ggaattcggc ggcaggaaac tggcatccga
     1921 cgaaaaggaa gagcttcttc ttgaactttt cccggctgtt gcaaaaccgt ttctgaaagg
     1981 gaaaagagaa ttggaatttc tcaatgagat cagggaaata caggaaagga cagacaaaga
     2041 aagaggcgtt ctgatgacag cccttgaaga aaactttctt atgtagagat aaatgaacga
     2101 aaatttaaac cagtatataa actcagaaaa aaaactgaag atcaggatcg aaaaactcct
     2161 ttcaacgctc catctttcaa aattctcggg cactgtaaat gttatcaacg gagatgacag
     2221 gttcaacatt attgttacag aaggaaaatc ctactgtgaa agatcccttc ttcagatttc
     2281 aacgctccct tccggaacac tttttatgct caaaacagaa cttcctgaag gattcaaggt
     2341 gaatctgaag gaatatgatt cactcgattt catattcagg atagttgaaa atatagaacg
     2401 atccatcatt gtagccatga taaaacctgt tgcgactgaa catttctcaa tcaggccgga
     2461 agcaagttcg atgacatttc ttcatgatga acttgaatct ttcatcggcc agaaaaggat
     2521 tgaaatattc tccagtttca aaaaacatta cagaacaatt ttcttccttc tgctgacaag
     2581 atttgcaagt gtcgtgcgat ccaccgatga agaaagggca aatatacagg aaatgctttc
     2641 acgctcgaac atgtttgatg aaaaggcaac tgaagaagag accgcagagg aggtcattga
     2701 gcagagcgaa gatcaaaagc tcgtaagttt tcttgatatt aatgaaaaat tcaaagatgt
     2761 ataccacctg ttcgggctct cttttgattt tgatgaaaaa gcacttcaca atacatatct
     2821 caagatcgtt cagaagattc accccgatgc actgcgcggt gtcaatgttg tcaacagaga
     2881 ccgtgcggaa aaattcttcc gggttgtcag tgaaaattat gaaatgctta gaaatcctga
     2941 agaaagggct gatgttgaaa agcttatgaa aagatacgga cccataaaaa gcaggaccga
     3001 tcttacgcgg atgaaagaat atgatgaagc tattttcaaa gggaccgcgt tgctccgtct
     3061 cggcaacttt gaaggtgcgg cagagatatt cggaatgatc tatgaccaga ccaaaaatcc
     3121 ggatgcactt gaaaaaaaga tcctcgcaat atggaaaaag tcccctagat ggaacgactt
     3181 tgaaaagact atcaagtatc ctccgataaa agaggatatt cttaaactca caatgataaa
     3241 ggaccctgat atcgaaatcc ttttcatcct cgcagagata tatgaatttc tcaaaatgat
     3301 ccccgactgc atgaaggtac ttaccataat aactaagatc cagccggaca acttcagggc
     3361 aaaagggatg aaaaaacggc ttatgtacta ccaatctctt gaaaaacagg gaaaaaaatg
     3421 agcatcttca ttaaagcttc tgtttttttt cttatgatga cttctctact atattcaagt
     3481 gtcagtatct attatttcta cgacccgatc aaggatagat acacattcac aaatcaatgt
     3541 tcagatctcc gctcatgcaa gcccctttat cagtcaaaag gtgaagtgaa acataaacgg
     3601 ggttatgcac tgaagccggg aaaagaatca gcttttgatg atataataca gaaagcttca
     3661 gttaaacaca gcgttgactt ccatctcata aaaagtgtca ttaaagcgga atctcttttt
     3721 gacacaaaag ctgtttcttc ggcaggagcg aagggactta tgcagttaat gcctgcaacg
     3781 gcggttgagg tcggcgttaa agatgtcttt gatgctcagc agaacataat gggaggcact
     3841 ctttatctca agaaaatgat caaacggttt aaaaacacaa gaagcgccct tgccggttat
     3901 aatgcgggac cggcggcagt tgtttattat aacggaatac cccctttcaa tgaaacagtt
     3961 gcatatgttg aaaaggtgtc tgacttttac aaaaactaca caggcaggga actatggtaa
     4021 aaaggtttag aaaaaataag aaaagcacag gtgatttcgg cagaagaaaa gtgatcatgg
     4081 acaagctcat caatttcaag aaagatgtcc ttacaattcc taatcttctt accggcatca
     4141 gaatattctt tgttccgttc gttctgtttc tgatatggta tgacacacct gtttcacggc
     4201 tgtggtcatg gatattttat acaatagcga cagttacaga tttttttgac ggatatatcg
     4261 caagaaagca cgggacggtc acagtgacag gcaaacttct tgatccgctt gcagacaagt
     4321 tcattgtcaa ccttacactt gtccttcttg tgtcgatggg tgaaatgcat gtccttccag
     4381 tcctcataat ccttgcacgc gaattttaca ttttcgggat tcgttctgtg gccgttgaaa
     4441 gaggcgttgt tgttgcggct ggtcagggtg gcaaaatgaa aacaatgttc cagatgtttg
     4501 ctcttccctt tttcatgatt aatcagaata cattgatagg gcttttcaat atcgactggg
     4561 acccgagatt atggggatat attcttatct ggggatcaat attcttcagt gtgacaagtg
     4621 cctacacata ttcgaaagaa gttaagagaa aacttttcag aagctagctg ttttccgatc
     4681 ttgccagaca cttattaaat tcctgaactg aaaccgaacc gtcaaaatat tttccaagag
     4741 gtttgaagaa tctgtttcca tcatttttta aaaccattaa tgcaacattg gcagcggtgg
     4801 aaacggcaat cggaacaccg ccgccgagag cagaccagtg accgcataga aacacatttt
     4861 caagcggcgt tacaaaatgg gcgatcctgg ctttaaaatt tacttttccg ggacgcgctc
     4921 ccatcatcac accttctctg ttccctgtgt agcggtgata tgtaacaggc gttgcaacat
     4981 ccatgaactc aatatgagac ctgatgtttt ccgatagtgc tttttcaact cgatttatga
     5041 gaatatgagc gaaatcattt ttgaatttca catattcatc tttggataag gtgctgaaat
     5101 cactataata tgcagggcag tatattgtga gtgtcccttt tccgtcagga gccattgatt
     5161 tatctcttgc tgaaggagac ataactgaga tgtcgctttt ttgagg
//
LOCUS       DAOOYB010000200         5126 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_18883_length_5126_cov_10.012818, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000200 DAOOYB010000000
VERSION     DAOOYB010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5126
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18883_length_5126_cov_10.012818"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            125..1357
                     /locus_tag="PL056_10535"
     CDS             125..1357
                     /locus_tag="PL056_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47492.1"
                     /translation="MRKRLFFVLVVITALFLISNCTSDDPEDGYAAGESCSTEAQEVC
                     SGDGSSILVCQTGIFVVKKNCNINFGEYCRLLATGPSCKDTGDTGNTGNTGDTGNTGD
                     TGNTGNTGNTGNTGDTGDTGNTGNTGDTGNTGNTGNTGNTGNTGNTGDTGDTAPEKGE
                     CEKIMDCMELCAEGATACMQTCYDNGSVAGKADYDAFWACDSTNNCQGNYLCNWENCQ
                     EKAKLCGLGGDTRYSVPYGTATLNGSFTYIVTNETSLSSNMYITKFIEGTFGTAGHLI
                     DPTAAGTFAYAQWIVPTTGSNFLALSQEYTDAILKNPMPYLVIDEADIKTGSHNVGLT
                     ATDTVQIFVAETDVDGNITCRHAFGVGTINISSINTSAGAAGNVSLTGTVQLYSPAAA
                     PIYGGDITSASMVACQPK"
     gene            1393..2487
                     /locus_tag="PL056_10540"
     CDS             1393..2487
                     /locus_tag="PL056_10540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47493.1"
                     /translation="MSEKTNSSGSTSRLLQLCFGYFIFYVINGVAVKFFLSTGEGYPA
                     LNGMEYLVYNTAGAALLATGVVLVLKWYRIESVRKINIMGISIPSELPYILVSGICTA
                     VIVPTTTLLYSFRGISVMVAMIIMRGSVIIIGRVVDAIQIKRGILKKKVYLEENIAMF
                     IALFAVSIKIFSGSGDGASPFTSIPVMVIFGSYIVSYAIRIYIMNYYKNTRPPEEKKD
                     NNKSFFAIEQITSFITMFLVTAAVLVAAKNFGVTGDRITPFSNAFFETNTEWAPWAFF
                     AGSAYGIIAFLSVFIFMFKGRTATFAGLANRLTSLIAGTVSTLVIAIFFSGKFPAIID
                     WVSLSFIFIAIAFMSKAEKKRKCEIGLQKC"
     gene            complement(2475..2999)
                     /locus_tag="PL056_10545"
     CDS             complement(2475..2999)
                     /locus_tag="PL056_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47494.1"
                     /translation="MIDFAVIEIAIFFLSVPFILYFFTSTLMFRSLTASAVSSLLLEV
                     INEKVFGSVGTYYPGSLMFFPFFKFPIAIIFLSVFYAGVINFVSLKISGFFVNKYVSI
                     SAFLISVFSLNSISIYVEKAGIYSGYWVHRQPAGISGIHGYVYLFYLSIVLAGSLFIV
                     SGSLKKFKKTDQHF"
     gene            complement(3010..4182)
                     /locus_tag="PL056_10550"
     CDS             complement(3010..4182)
                     /locus_tag="PL056_10550"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPM47495.1"
                     /translation="MNKTVFALLFIVVSAVAVFFSREFHFIENYLKLSPQIIYAAAGL
                     AFLICLRFNKSRAAFMLFLILLWFFKDNPGFVSKIPENEFMFFITFNVLYITTSKERG
                     ILSIHGLKKVLFIVSQFWLIYYFTIYQPTYYNQSANSVIKTIQFMMGMQFHFLPFVML
                     GLAVVRNIFSDKSYEVSTALGISIAFLILTTLKIDLSSLNLLTGFALIFTGILSSIYS
                     TSYTDELTGLPARRAYNEYTSALGAKYAIAMTDIDHFKKFNDTYGHDTGDEVLKLVAK
                     VISSVGGGGKAFRFGGEEFVIIFSGKTKEETAEHLENIREKLQNTPFTVRNRKSRAKY
                     KKTGRKGNSVSSKTVRITMSIGASDSRAEKTVAKVMKKADQQLYKAKKAGRNRVVA"
     gene            complement(4179..>5126)
                     /locus_tag="PL056_10555"
     CDS             complement(4179..>5126)
                     /locus_tag="PL056_10555"
                     /inference="COORDINATES: protein motif:HMM:NF015746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HPM47496.1"
                     /translation="HFRRFLSSFLLKIERFRYYFMAASAFIIVITGTFITEKIIEPRL
                     GKYKGAEEVKTDEKISPEEKKGLIYAGITFLIFFLILAAGIIPETGFLRDHAHNNAVL
                     TSPLIKGIITVIFLIFVLAGIAYGIGCGKFRSDSDIVAGMENSMKSMGLYIVLAFFAA
                     QFVSFFNWSRLGVVIAVNGAEFLMSLKLGLIPLVILFVFMSAFFNLFIGSASAKWALL
                     APIFVPIFMILGFSPELSQAVYRIGDSTTNIISPMMSYFALIIAFIRKYDKDAGIGSI
                     ISLMIPYTMAFMLLWTILLIVWIELGIPLGPGAGIFYKP"
BASE COUNT         1607 a          990 c         1063 g         1466 t
ORIGIN      
        1 gctcctcctc ttcagttaga aatctgctga attttttttg ggggaacttc aaaatccttc
       61 ttcttgacat aagaaggtaa atacctatca ttataaggat gtgtgtaatt ttaagggggt
      121 tattatgaga aaaagattat tttttgtttt agttgtaatt acagcattgt ttttaatttc
      181 aaactgtaca tcggatgatc ctgaagatgg atatgcggca ggagaaagct gttctactga
      241 agcacaggaa gtctgttcag gtgacggttc ttcaattctg gtctgccaga cagggatttt
      301 cgttgtcaaa aagaactgta atattaattt cggggagtac tgtcggttac tcgcgaccgg
      361 cccatcctgt aaagatacgg gagataccgg caatacagga aatacgggtg acacaggcaa
      421 tacaggagat accggcaata caggaaatac aggcaacaca ggcaatacag gagatacggg
      481 agataccggc aatacaggaa atacgggtga cacaggcaat acaggaaaca caggtaacac
      541 aggcaataca ggcaatacag gaaacacagg tgacacgggc gacacagctc ctgaaaaagg
      601 tgagtgtgaa aaaataatgg attgtatgga gctttgtgcg gaaggagcta cagcatgcat
      661 gcagacttgc tacgataatg gatcggttgc cggaaaagct gattacgatg ctttctgggc
      721 atgtgattct acaaataatt gtcagggcaa ttatctttgt aactgggaaa actgtcagga
      781 aaaagcaaaa ctttgcggtc tcggaggaga tacaagatac agtgtgcctt acggtacagc
      841 gactctgaac ggaagcttca catatattgt tacaaatgaa acatctcttt cgagtaatat
      901 gtatatcaca aaatttattg aaggtacttt cggtacagcc gggcatctta ttgatcccac
      961 ggcagctggc acatttgcct atgcacagtg gatcgtgcct acaacaggaa gtaattttct
     1021 ggctctctcg caggaataca ctgatgcgat cctgaaaaac cccatgccct atcttgtaat
     1081 tgatgaggcg gacataaaaa caggcagtca caatgtcgga cttacagcta cagatactgt
     1141 ccagatcttt gttgcggaaa ctgatgtcga tggaaatata acttgcagac acgctttcgg
     1201 ggtcggtacg ataaacattt cctcaataaa tacctcagcc ggtgccgccg gaaatgtttc
     1261 tttaaccgga actgttcagc tttacagtcc ggcagcggct ccgatctatg gtggagacat
     1321 tacaagtgca agtatggttg cttgtcaacc gaaataaaat tcttttccaa ttgatttttc
     1381 aaggagaata ttttgtctga aaaaaccaat tcctcgggtt ctacatcacg gcttcttcaa
     1441 ctctgtttcg gatattttat tttttatgtc attaacggag ttgcggtcaa gttctttctt
     1501 tcaacaggag agggttatcc ggcccttaac ggaatggagt atcttgtcta taatacagcc
     1561 ggagcagctc ttctggcgac cggagtagtt cttgttctga aatggtacag gatcgaatct
     1621 gtcaggaaga tcaacatcat ggggattagt attccgtctg aactgcctta cattcttgtt
     1681 tcaggaatat gtacggccgt aattgttccg acaacgacac ttctttattc tttcaggggt
     1741 atctctgtca tggttgcaat gataataatg aggggctcag taataataat cggcagggtg
     1801 gtagatgcca tccagataaa acggggaata ttgaaaaaga aagtgtatct tgaagaaaac
     1861 atagcaatgt ttatcgctct ttttgctgtg agcatcaaaa tattttccgg aagcggtgac
     1921 ggagcaagtc cttttacttc gatccctgta atggtgatct tcggaagtta tatcgtttca
     1981 tacgcaataa gaatttacat aatgaactac tataaaaaca cccgcccgcc ggaagaaaaa
     2041 aaggataaca acaaatcgtt tttcgcaatt gaacagatta catctttcat aacgatgttc
     2101 cttgttacgg cagcagtgct tgttgctgct aaaaactttg gtgtgacagg agacagaata
     2161 acaccttttt caaatgcatt ctttgaaacg aacacggaat gggcgccttg ggcttttttt
     2221 gccggttctg cttatggaat aattgctttc ctttctgttt ttattttcat gtttaaagga
     2281 agaacagcaa cattcgcagg acttgccaat cgcctgacat cactaattgc aggaaccgtt
     2341 tcaaccttgg tgatagcaat tttcttcagc ggaaaatttc ctgctattat tgactgggtt
     2401 tcactcagtt ttatttttat tgccattgcc tttatgtcaa aagccgaaaa gaagagaaaa
     2461 tgtgagatcg gccttcagaa atgctgatct gtctttttga acttttttaa actcccggaa
     2521 acaatgaaaa gagatcctgc aagaacgatt gagagataaa aaagatagac atatccgtga
     2581 atgccggaga tccctgcggg ttgtcggtga acccagtatc ctgaataaat tccggctttt
     2641 tccacatata ttgatattga attcagtgaa aagacagaga tcagaaaagc agagatcgaa
     2701 acatatttat taacaaaaaa cccggatatt ttcaaagaaa caaaatttat aacacccgca
     2761 taaaagacac tcaagaaaat aatggcaatc ggaaatttaa agaacgggaa aaacatgagc
     2821 gatcctggat aatatgtccc gacagagccg aagaccttct cgtttatcac ttcaagcagc
     2881 agtgatgaga cagcagaagc agtcaaagat ctgaacatta gtgtcgaagt gaaaaaatag
     2941 agaataaatg ggaccgacag aaaaaagatc gcaatttcta tcacagcaaa atcgatcatt
     3001 aaaccctcct tatgcaacga ccctgttcct tcccgctttc tttgctttat aaagttgctg
     3061 gtctgctttt ttcatcactt ttgcaactgt tttttcagct cttgagtcgc ttgcgccgat
     3121 gctcattgtg atccggactg ttttcgaaga aacggaattt ccctttctgc ctgtcttttt
     3181 atattttgcc ctactttttc tgtttcttac cgtgaaaggg gtgttctgta gcttttctct
     3241 tatgttttca agatgttctg cagtttcttc tttggtcttt ccactaaata tgataacaaa
     3301 ttcctctcct ccgaacctga atgcttttcc tccgcctcca actgaagaaa tcacttttgc
     3361 gacaagtttc agaacttcat caccggtatc atgtccgtat gtatcattga attttttgaa
     3421 atgatcgatg tctgtcattg caattgcata ttttgcccca agtgccgaag tgtattcatt
     3481 atatgccctt cttgcaggaa gacctgtaag ttcatcagtg taggatgttg aataaattga
     3541 agaaagaatg cctgtgaaaa taagagcaaa ccccgtgaga agattaagag aactcagatc
     3601 aattttcaaa gtcgttaata tcagaaaagc aattgatatt ccgagtgctg ttgaaacttc
     3661 ataactttta tcactgaaaa tgttccttac tacggcaagt ccgagcatca caaaagggag
     3721 aaaatggaac tgcataccca tcatgaattg aattgttttt ataactgaat ttgcagactg
     3781 attgtaatat gtcggctgat aaattgtaaa ataatagatc agccagaact ggcttacaat
     3841 gaacagcact tttttcagac cgtgaataga tagtatacct ctttcttttg atgtagtgat
     3901 gtataaaaca ttaaatgtaa taaagaacat aaattcattt tcagggattt ttgaaacaaa
     3961 gcccgggttg tctttaaaaa accacagaag aataagaaaa agcatgaacg ccgctctgct
     4021 tttgttgaat ctcaggcaaa ttaaaaaagc cagtcctgcc gccgcataaa ttatctgagg
     4081 agacaactta agataatttt caatgaaatg aaattctctg ctgaagaaaa cagcaacggc
     4141 tgaaacaaca atgaataata aggcaaaaac ggttttgttc atggtttata gaagatccct
     4201 gccccgggtc cgagaggtat tccaagctct atccagacaa taagcagaat tgtccacaat
     4261 aacatgaatg ccatagtgta ggggatcata agtgaaatta ttgatccgat tccggcatct
     4321 ttgtcatatt ttctaatgaa tgcgataatt aacgcaaaat aactcatcat ggggctgatt
     4381 atgttggttg tcgaatcacc gattctgtat actgcctgtg aaagttcagg tgagaatccg
     4441 agtatcatga aaatggggac gaaaataggg gcaagaagtg cccattttgc cgaagcactt
     4501 ccaataaaaa gattaaaaaa tgccgacatg aagacaaaga gaatgacaag cggtatcagt
     4561 cccagcttta aggacatcaa gaattcggca ccattcactg caattactac tccgagccgg
     4621 ctccagttga aaaaggagac gaactgagcg gcaaaaaaag caaggacaat gtaaagcccc
     4681 attgatttca tggaattttc catgcctgca actatgtcag aatcgcttct gaactttccg
     4741 cagccaattc cgtaagctat tccggcaaga acaaaaataa gaaaaatgac agtgatgatc
     4801 ccttttatca ggggagatgt gaggacagca ttattatgag catggtcacg cagaaatccg
     4861 gtttcaggaa ttatgcctgc cgcaagaatt aaaaaaaata taagaaaggt gatgccggca
     4921 taaatcagtc cttttttttc ttccggcgag attttttcat cagttttaac ttcttcagcc
     4981 cctttatatt ttccaagacg cggctcaatg atcttctctg ttatgaaagt tccggttatg
     5041 actattatga aagctgatgc agccataaag taatacctaa atcgttcgat tttcaataaa
     5101 aaacttgaca aaaacctccg aaagtg
//
LOCUS       DAOOYB010000201         5114 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_18942_length_5114_cov_9.394742, whole genome shotgun sequence.
ACCESSION   DAOOYB010000201 DAOOYB010000000
VERSION     DAOOYB010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5114
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18942_length_5114_cov_9.394742"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="PL056_10560"
     CDS             <1..406
                     /locus_tag="PL056_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47497.1"
                     /translation="EITNSYAIVERDGEYQTEIVQAQFPLLMTVTSAVNTPRYPNAAK
                     MLKYLKSDIASAIPADKLEKMEKAGKVIPLLTNEDLKLESEKCGLKGSPTKVHNIKSI
                     TLTGGDLKIYGNENEGIKSLIRDIMKDYVEVE"
     gene            406..1398
                     /locus_tag="PL056_10565"
     CDS             406..1398
                     /locus_tag="PL056_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HPM47498.1"
                     /translation="MKVLVFIEKNTHTDVLRNVTAELTWKAHSLVKPLNGEVVGIFAG
                     DKLPQDHAELFKYGMDRILYFNHPQLKNLQSIAMKNILTEMVKSENADMVLFGATHTG
                     RDVAPLIASTLRTGLTADCTQLYIDEYKESGKILYQVRPAFGGNILATIITPYNRPMM
                     ATVREGVMLLPEEKVNKPAKLQEFSTEFKMEWIKNEFLEIIPKVSKVNFGEANIIVAG
                     GAGVGCRENFRLLEQLAAVLNAEVGASRAAVDFGFAEKERQIGQTGSVVRPKLYIACG
                     ISGSIQHRAGMEESKKIIAINSDPNAPIFNIAHMGIVGDLKEIIPVMIKYLREE"
     gene            1399..3102
                     /locus_tag="PL056_10570"
     CDS             1399..3102
                     /locus_tag="PL056_10570"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPM47499.1"
                     /translation="MSGFYNENKRFYLSHIDLKEIIHDLENNFIGDDDNAPSSYEEAL
                     EWYQLALENAGKICEEFIAPRAEHVDEKGASYCGGVVKWAPETYENMHALAKAGYMGG
                     TLPRKYGGLNMPVTVNNIIVEMVSQADASLMNLVGLQDIAVTLYKFGNEEQKNRVLPK
                     FCSGEVSGSMALTEPDAGSDLQAVALKAIEKDGKWYLDGVKRFITNGNGDVSLVLARS
                     EEGTKDGRGLSMFLYERYRDDNMIIRRIEDKHGIHGSPTCELQFNMGECELVGKRRFG
                     LIKYVMSLMNGARLAVSAQAMGIAQSAYDEAVKYANEREQFGKAIVHFPAVYQMLIDM
                     KAEIETTRLLLYHTSLSVDYMDMYERKAEHGESVRDQLKSWTSKADFLTPLTKFTATE
                     MANKVAYDAVQIHGGCGFMKDFKVERLSRDARITNIYEGTTQLQVVAALPRITSGLFR
                     DMVKELLAADDIKDQEISRNILETVEKVMKSVETVKVNENKDLLDYCGNYFVETASIL
                     YRLSLFVPVAQKHEEKQELFKYFTHKSFNRINYLISMIESLEKSYGKSIDSLKKPFVK
                     G"
     gene            complement(3092..4969)
                     /locus_tag="PL056_10575"
     CDS             complement(3092..4969)
                     /locus_tag="PL056_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47500.1"
                     /translation="MKKFFVIFMISVISVIFTTGCDEEVKAGKDGKSMLTLLSDEESG
                     ENCANGGIRIDTGFDSDSDGALSEEEINDTKFVCDGTAGTDGTEGVDGEDGVNGEDGD
                     KGDQGDPGVDGEDGVDGENGVCAGNVAPVIESIVINGETYSGTPIILLDNSAEVVVTV
                     SKPEAELIITGGNASIEQTANTFKITLGADGSYHFGIIASDGCQMDVENFAVRSPLKC
                     AGDITINNETQLDAIKDCEMIEGYLTITNYTADTFELENLLSVQGEFSIYDNSTLTSF
                     SLPYFKVSGGDFWINYNPELLSVDLPEYTTAEGYFWVFENDKIESVYADKLESAGGSF
                     GISNNPMLKEFSAKNLKSTEAGVWIYNNGKLESFELPALKKVGSYIYIYQNDLLAEFS
                     LPALETVGEGLQIHESPALTAILFPALKNIGFGFLAMTNFSVETIDLPELTRVGGGVR
                     IETQTKLTSFKANKLESIALDQGCGFYFYDNPLATTVEMNTLKEVGEDFYVYNNEALT
                     YFEMPALETVENSIYIYGNILLDSFDFPTLTITGGLSVYGNEALNKFKMALLEGIKGD
                     LLINKNVILTGFDLSTLADVGGNFTITDNVELPTLTAQDLADQVTIGGTTTISGNKPT
                     P"
BASE COUNT         1580 a         1020 c         1102 g         1412 t
ORIGIN      
        1 agagattaca aatagttacg caattgttga aagggatggt gaatatcaga ccgagatagt
       61 gcaagcacag tttcctcttc tgatgacagt aacttccgct gtgaacactc caagatatcc
      121 caatgctgca aaaatgctta aatatcttaa aagcgacatc gcttccgcaa ttcctgccga
      181 caaactggaa aagatggaaa aggcaggtaa ggtgattccg cttcttacaa atgaagatct
      241 aaaactcgaa agtgaaaagt gcggtcttaa aggaagtccc acaaaggttc ataacataaa
      301 gagcatcacc cttaccggcg gagatctcaa gatatacggc aatgaaaatg aggggataaa
      361 gtcgctgatc cgtgacatca tgaaagacta tgttgaggtg gaatgatgaa agtacttgta
      421 tttattgaaa aaaacacaca tactgatgtg ctcagaaatg tcactgcaga acttacatgg
      481 aaagcacact cccttgtcaa gccgcttaac ggagaggtcg tcgggatatt tgcaggtgac
      541 aagcttccgc aggaccatgc tgagcttttt aagtacggaa tggacagaat cctttatttc
      601 aaccatccgc aactgaaaaa tctacagtcc attgcgatga aaaatattct gacagagatg
      661 gtgaaaagtg aaaatgccga tatggttctt ttcggtgcga ctcacactgg tcgtgatgtc
      721 gcccctctga ttgcttcgac tctaaggaca ggacttactg ctgactgcac acagctttac
      781 attgatgaat ataaggaaag cggaaagatc ctttatcagg tgagacctgc tttcggcggc
      841 aacattcttg caaccatcat cactccgtac aacaggccga tgatggcaac tgtaagagaa
      901 ggggtaatgc ttcttccgga agaaaaggtt aataagccgg caaagcttca ggagtttagt
      961 accgaattta aaatggagtg gataaaaaat gaatttcttg agataattcc gaaggtttcc
     1021 aaagtcaatt tcggtgaagc caatatcatc gttgcaggtg gtgcaggtgt cggatgcagg
     1081 gagaatttcc ggcttcttga acagcttgcg gcggttctta acgctgaggt gggagcgagc
     1141 agagcggctg ttgatttcgg ttttgccgaa aaggaaaggc agatcggtca gaccggttca
     1201 gttgttcggc ctaaacttta tatcgcatgc ggaatttcgg gttccattca gcatcgtgca
     1261 gggatggagg agtcaaagaa gataatcgcc ataaattccg atccgaacgc tccgatattc
     1321 aacatcgccc acatgggtat agtcggagat ctgaaagaga taatccctgt tatgataaaa
     1381 tatttgaggg aggaatagat gtctggattc tacaatgaaa acaagaggtt ctatttgtcg
     1441 catattgacc ttaaagagat aatacacgat cttgagaaca attttattgg tgacgatgat
     1501 aacgctcctt caagttacga agaagcgctt gagtggtatc agctcgccct tgaaaatgcg
     1561 ggtaaaatct gtgaggagtt cattgctcca agagcagaac atgttgatga aaaaggggca
     1621 tcgtactgcg gcggagttgt gaagtgggct cctgaaacat acgaaaacat gcatgctctt
     1681 gccaaagccg gctatatggg tggaactttg ccaagaaaat acggcggact caatatgcct
     1741 gtcaccgtga ataacattat cgttgaaatg gtttcccagg cggacgcttc gctgatgaat
     1801 cttgtcggtt tgcaggatat cgctgttaca ctttacaaat tcggaaacga agaacagaaa
     1861 aaccgtgttc tgccgaagtt ctgcagtgga gaggtaagcg gttcgatggc tctgactgag
     1921 cccgatgcag gaagtgatct tcaggcggta gcacttaaag cgatagaaaa ggacggaaag
     1981 tggtatcttg acggagttaa aaggttcata acaaacggaa atggtgatgt ttctcttgtt
     2041 cttgcaagaa gcgaagaagg gactaaggac ggtcgcggac tgtcgatgtt cctttacgaa
     2101 agatatcgcg atgacaacat gataatcagg cgtatcgagg ataagcacgg tattcatggt
     2161 tctccgacat gcgagctaca gttcaatatg ggagagtgtg aacttgtcgg caaaagaagg
     2221 ttcgggctta taaaatatgt catgagctta atgaacggag cacggcttgc agtttcagct
     2281 caggcgatgg gaattgctca gtcggcctat gatgaagcag tcaaatatgc aaacgaaaga
     2341 gaacagtttg gcaaagcgat cgttcatttt cccgcggtat atcagatgct tattgatatg
     2401 aaagcagaaa ttgaaacgac acggcttctc ctttatcata cttctttatc agttgattat
     2461 atggatatgt atgaaaggaa ggcggaacat ggtgaaagtg tgagagatca gcttaaatca
     2521 tggacatcaa aagccgattt tcttactccg cttacaaagt tcacagcaac agaaatggca
     2581 aacaaagttg cttatgatgc cgttcagatc cacggcggat gcggtttcat gaaagatttc
     2641 aaggttgagc ggctcagcag agatgcaagg atcactaata tttatgaagg gacgacacag
     2701 cttcaggtcg ttgccgcact gccgagaatt acaagcggac ttttcaggga tatggtgaaa
     2761 gaacttcttg cggctgatga cataaaagac caagaaataa gcaggaatat ccttgaaacc
     2821 gttgaaaaag ttatgaagtc agttgaaact gtaaaagtca atgaaaacaa agaccttctt
     2881 gactactgcg gcaactattt tgttgaaacc gcatcaatac tctaccgttt gtccctgttt
     2941 gttccagttg cacagaaaca cgaagaaaaa caggaacttt tcaaatattt cacacacaaa
     3001 tccttcaata gaataaatta tctcatttct atgatagaat cactcgaaaa aagttacgga
     3061 aaatcaatag attcgcttaa aaagccgttc gttaaggggt aggtttattc ccggaaattg
     3121 ttgtggttcc gccaatcgtt acctgatcag caagatcctg tgcagttaat gtcggaagtt
     3181 caacattgtc agtaattgtg aaattgccgc ccacatccgc aagagttgaa agatcgaaac
     3241 cggtgagtat cacattcttg tttatgagca gatcaccttt tattccttca agaagagcca
     3301 ttttaaattt attaagagct tcattcccat aaactgaaag ccctccggta atagtaagag
     3361 tcggaaagtc aaacgaatca aggagtatgt tgccataaat ataaattgaa ttttcgactg
     3421 tttcaagtgc cggcatttca aaataagtta aggcctcgtt gttgtaaaca taaaaatctt
     3481 caccgacttc ttttaatgta ttcatctcaa cagttgtggc cagggggttg tcataaaaat
     3541 aaaatccgca gccctgatca agagcaattg attcaagttt gtttgcttta aaagaggtaa
     3601 gttttgtctg agtttcaatc cttactccgc caccgactct ggttaactca gggaggtcaa
     3661 ttgtttcaac tgaaaaatta gtcattgcca agaacccgaa acctatattt tttagtgccg
     3721 ggaaaagaat tgctgtgaga gcggggcttt catgtatctg caatccttca ccaacagttt
     3781 caagagctgg taagctaaat tcagcaagaa gatcgttctg gtaaatgtaa atatatgaac
     3841 cgactttttt aagtgccgga agttcaaatg attcaagttt tccgttatta tatatccata
     3901 cacctgcctc tgtggatttc agattctttg ctgaaaactc tttaagcatc gggttgttgc
     3961 tgataccgaa agaacctccc gcactttcaa gtttgtcggc ataaacactt tcaattttat
     4021 cgttttcaaa aacccagaaa taaccttctg cagtcgtata ttccggcaga tcaacactca
     4081 gaagttcagg attgtaattt atccagaaat ctccgcctga cactttaaaa tatggaaggg
     4141 aaaaagaagt aagagtggaa ttgtcataaa tactaaattc tccctgaaca gataacaaat
     4201 tctcaagttc aaaggtgtcg gcagtgtaat ttgttattgt taaataaccc tcgatcattt
     4261 cacaatcttt tattgcatca agctgggttt cgttgttgat cgtaatatct ccggcacatt
     4321 ttaaggggct tctcacagca aaattttcta catccatctg acatccgtca gaagcaataa
     4381 taccaaaatg atatgatccg tctgctccca aagttatttt gaaagtgttt gcagtctgct
     4441 caatagatgc atttccgcct gttatgataa gttcagcttc aggtttactg acagttacga
     4501 caacttcggc actgttatca agaagtatga ttggtgtgcc gctgtaagtt tcaccgttaa
     4561 tgacgataga ttcgataacc ggagcaacat tgcctgcaca aactccattt tcaccatcca
     4621 caccgtcttc accatctaca cccggatcgc cctgatcacc tttgtctcca tcttcaccgt
     4681 tcacgccgtc ttcaccgtca acgccttcag ttccgtcagt tccggcagta ccgtcacaaa
     4741 caaatttggt gtcgttaatc tcttcttcac taagtgcacc atcgctgtca gaatcaaaac
     4801 cggtatcaat cctgatgcct ccatttgcac agttttctcc ggattcttca tcacttaaaa
     4861 gtgtcagcat acttttgccg tctttgcctg ccttaacttc ttcatcacag ccagtcgtaa
     4921 agattacgga aatgactgag atcataaaaa ttacaaaaaa ttttttcatt acaaaacctc
     4981 caaaaaaaat ggtaaaaagt caattcttaa aattgtttcg ttaaatcgcg aaaataatat
     5041 gaaaattttt gaaacaattc aagtttaaat tcattttctt gataaaatct cattttttta
     5101 gataatttta acct
//
LOCUS       DAOOYB010000202         5080 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_19118_length_5080_cov_11.857512, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000202 DAOOYB010000000
VERSION     DAOOYB010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5080
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19118_length_5080_cov_11.857512"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(279..455)
                     /locus_tag="PL056_10580"
     CDS             complement(279..455)
                     /locus_tag="PL056_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47501.1"
                     /translation="MSEKKVVVSKTQDVLITFLKEVQDGVYAPVEKSSLPYSRMSEQA
                     EDLSCSEEYIYSIR"
     gene            complement(478..1668)
                     /locus_tag="PL056_10585"
     CDS             complement(478..1668)
                     /locus_tag="PL056_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007780523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HPM47502.1"
                     /translation="MSFMKEYIDKRLSVKDLENELAVLMRKYNKLRNTYLFVYSTAIR
                     KNIPQVALEQDDFYIIYDILSKLPNTKKIDFYIETPGGSGEAAEEIVRFLRKKFDHVS
                     FVVSGESKSAGTLIVLSGDEILMTDSASLGPIDAQVRIGRSVVSAHNYMQWIDEKREE
                     AARIGALNPFDATMIAQISPGELLGVSHSLDFAERLVKEWLPKYKFRTWTVTETRRLE
                     VTDQMRQERADDIASKLTDHSRWLSHGRSLKIEDLNKIGLEIIRVDENQNLSDIVYRI
                     QTILNLIFSSSTSFKIFATDKNRIFKQAVAARSSMNIPNAKQSDKPMSVATFEIKCAQ
                     CSEVHKIYAKLINDEKIDLNLKKKGFVPFPKDAKLKCRCGFEIDLMGVKNELELKIGR
                     KIII"
     gene            1919..2200
                     /locus_tag="PL056_10590"
     CDS             1919..2200
                     /locus_tag="PL056_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47503.1"
                     /translation="MNEFEIMEKANKISTQVTLLSAHRQFREAIDYGKAELLKHPEFS
                     DEDKTIVYIPMMKAAAEAGFRHEAVEYAKKVAITDPDMPSVKKVFKLYL"
     gene            complement(3167..4033)
                     /locus_tag="PL056_10595"
     CDS             complement(3167..4033)
                     /locus_tag="PL056_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47504.1"
                     /translation="MKYNSSENVLEIIARTGGVFNTEILDKIFNADSFVKYLKRKNEL
                     EYISKQGVANYIYKIPDKYIREYHNVRWVRSKDLNSKGELEFRKRVVKYLRENGIKGK
                     YNRFQGERFYKEIFKEMFTPENEKIYWNYYFDGLNENHIIYSFAMQLLYISDCIIPYT
                     KGLLTENFPDYFQKYSGKGMYFSIVEDYRGAERLGCVIIESMKPYDSIFRILEKFGKF
                     PLLKDRCLFYIVSGNKNSLKTLENRIKNYRKSITDNSGNLVRYEKNYFSEIRIQYRYY
                     EELDRILNRGVK"
     gene            complement(4036..4605)
                     /locus_tag="PL056_10600"
     CDS             complement(4036..4605)
                     /locus_tag="PL056_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47505.1"
                     /translation="MAYTWSKFQVGNYSQSEVNGFRGLANTGIIKLSDFKAAFTEKSY
                     ENMKSLKMIENKKYWVSGKETTVVRLTRTGKKFVKAAMVDKLYKYNPRQLTHDIKLSE
                     KYISLSDKERSTWVHEGKMAEQYEKMGIPKEKIESGEIQTVDGCYTNSAGEVVAVEIV
                     TSNYSTEKVNGKMAAVRYFSGGGIIDKVR"
     gene            complement(4830..>5080)
                     /locus_tag="PL056_10605"
     CDS             complement(4830..>5080)
                     /locus_tag="PL056_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47506.1"
                     /translation="GARAKLTDDRELEKEQKTNIIAPMDIAKRIFNLFKDIELNDEQV
                     REIKKHLRAIVKDPKYKERIFVDFSLATKKKDEDEEIQ"
BASE COUNT         1470 a          974 c          864 g         1772 t
ORIGIN      
        1 ggatgaataa ctcttggaaa tgtttgaaat gtaagaacga atgtccatat ctgccccgct
       61 tagaaaaatt cggttaaata tataaaatta aaagaattaa atcaacatta caattgatct
      121 ggtttgccgg gttcaatatc tgccgcatta aggctgtttt attgtcttaa cgctttaatg
      181 ctttaacgct tagttgctta atgtatgttc ttgtaaatac ttgaaatgta actcagattt
      241 gataaagcga gaaagaattt aattcaaaat cgcggacatc atcgaatact gtaaatatac
      301 tcttctgaac aagataagtc ctctgcctgt tcgctcattc tagaataagg aagtgagctc
      361 ttttctacag gagcataaac accatcttga acttccttca aaaaggtaat caaaacatcc
      421 tgcgttttac tcacaacaac tttcttttca ctcaaaacaa caccccttct acaaaaatta
      481 tattataatc tttctgccga tttttaactc tagttcgttt ttgaccccca taagatcgat
      541 ttcaaaaccg catcgacatt ttagttttgc atctttcgga aatggaacaa accctttctt
      601 tttgagattt aagtctattt tctcatcatt tataagcttg gcatatattt tatgaacttc
      661 ggagcattgg gcacatttaa tctcaaatgt agcgacactc attggcttgt cagactgttt
      721 tgcattagga atattcatgg aagagcgagc agcgacggcc tgtttaaata ttcgattttt
      781 atcagttgca aaaattttaa aggacgttga gctactgaaa atcaaattca aaatggtctg
      841 tatcctatat acaatatctg aaagattttg attttcatca actcttataa tttcaagacc
      901 tattttgttt aaatcctcaa tctttaatga tcttccgtga gataaccatc ttgagtgatc
      961 tgttaattta gaagctatat catctgctcg ttcctgacgc atctgatctg taacctctaa
     1021 tctgcgtgtt tccgtaacag tccatgtcct gaatttatat ttaggcagcc actcttttac
     1081 caaccgctct gcaaaatcca aagagtggct aacccccaga agttctccag gtgaaatctg
     1141 tgcaatcatt gttgcatcaa aggggtttaa tgcgccgatt ctagcggcct cttccctttt
     1201 ttcatcaatc cactgcatat agttgtgggc agaaaccacg gaccttccga ttctaacctg
     1261 agcatcaatg ggtccaagac ttgcagagtc tgtcattaaa atctcatctc cagacagaac
     1321 aattaaagtc cctgcacttt ttgattcacc ggaaacaaca aaagaaacat gatcaaattt
     1381 tttccttaaa aacctaacta tttcttcagc ggcctcacca cttccacctg gcgtttctat
     1441 ataaaagtct attttttttg tgttgggaag cttcgacaaa atatcgtaaa taatataaaa
     1501 atcatcttgc tctaaagcta cttgcggaat gttttttcta attgctgtcg aatacacgaa
     1561 caaataagta tttcttaatt tattgtattt acgcattaac acagcaagct cattctcaag
     1621 atctttaacg ctgagccttt tatcaatata ttccttcata aaactcattc tgtcactccc
     1681 gtgttcttac aacatactta aagtatccta acataaaaaa taattatttc aagagaactc
     1741 tcgcctttcc ccacaaaggc caaaaagctt tgaactattc tatattgctc ttttcagggg
     1801 ggtttgtttt aattcgatat agcgaacagt taattaagcg ccatgctcat ctttgttttt
     1861 aaaataaaat agataacatt gttgttaggt tcattaacaa ttttagatga ggtgaaagat
     1921 gaatgagttt gaaattatgg aaaaagccaa caaaatctca acccaagtga ctttgttgtc
     1981 tgcacacaga caatttcgag aggcaattga ttatggaaaa gctgagcttt taaaacatcc
     2041 cgagttttca gatgaggaca aaaccattgt ttacattcca atgatgaaag cagctgcaga
     2101 agcaggattc agacacgaag ctgttgaata tgcaaaaaag gtagctatta ctgacccaga
     2161 catgccatct gttaaaaaag tttttaaact ctatctctaa gatttggttt gaacatgttc
     2221 ccctgatctc cggaaacggt tttcagggag ttttgtttta attcgctata gcgataaatt
     2281 cactaaagcg aatttcaggt tttgcatttc cagcaatcat atcatctaaa tattccaaga
     2341 aacaagatct gtgaaaagca gaaaatctga ttttgttcct gctgttttcc cggaatttat
     2401 cttgccacga taacctgaaa accggaaggt tgagggttcg cgaaagtgaa aatattgagt
     2461 tgcaaggcaa ctaatatttc ggtgtaaccg caaatttttc agcagaaaaa ttccgaagta
     2521 tcacggagga attgccgaaa gcaattcgtc catccgaagg actgtgaacg gagtgaaaca
     2581 gggtgtaaaa gtgtaaacga tacacctgga cggattgcga gtttcagttt cgcgggatcg
     2641 tggcaagttt cttcaatgat tccgtttatt ttagtatgtt tttataattt gtttcaggtg
     2701 gtgttttata tccttttata agaggtctta caaaaggata taaaagtttc aggtttttaa
     2761 ggtttttacc ggattttatc ttgtcacgac aacctgaaaa ccgaagggtt gaaggttcac
     2821 aaaagtgaaa atattgagtt gcagggcaac taatatttcg gtgtaaccgc aaatttttca
     2881 gcagaaaaat tccggagttt acggaggaat tgccgaaagc aattcgccca accgaaggac
     2941 tgtgaacgga gtgaaacagg gtgtaaaagt gtaaacgata cacctgggcg gattgcagtt
     3001 ttagttttgt ggagtcgtga caagtttctt taatgattcc gtttatttta gttttttcaa
     3061 gggagtgttt tagtatcatt ttttgtggac ttaccatatt tatggtaagt cggactaaaa
     3121 gttttaggtt tttggggttt tttgaggaat atttttataa aaaagattat ttgactcccc
     3181 tgtttaaaat acggtcaagt tcttcataat accggtactg tatcctgatt tcgctgaaat
     3241 aattcttctc gtatctaaca agatttcctg aattatcggt tatgcttttt ctgtaatttt
     3301 taattctgtt ttcaagtgtt tttaaagagt ttttattgcc cgaaactatg taaaaaagac
     3361 acctgtcttt taagagcgga aattttccga atttctcaag aattctgaat attgaatcat
     3421 agggtttcat gctctcaatt atcacacagc cgagccgttc cgcccccctg taatcttcca
     3481 ctattgaaaa gtacattccc ttgccggaat atttctgaaa atagtcggga aaattttccg
     3541 tcagcagtcc ttttgtatat ggaattatac agtcggaaat ataaagaagc tgcatcgcaa
     3601 aactgtaaat tatgtgattt tcattaagtc cgtcaaaata ataattccag taaattttct
     3661 cgttttcagg agtgaacatt tctttaaata tttccttgta aaaccgctct ccctgaaagc
     3721 ggttgtattt tccttttatt ccgttttcgc gtaaatattt cacaactctt tttctgaatt
     3781 caagctcccc ttttgaatta agatcctttg atcttaccca tctgacattg tgatattccc
     3841 tgatatattt atcgggaatt ttatagatgt agtttgccac cccttgtttt gaaatatatt
     3901 caagttcatt tttccttttt agatatttaa caaaactgtc tgcattaaat attttgtcta
     3961 atatctctgt gttaaaaaca ccaccggtac gggctattat ttcaagaaca ttttcggaac
     4021 tgttgtattt catttttacc ttactttgtc aattattccg ccgccggaaa aataccttac
     4081 tgccgccatt tttccattaa ctttctctgt gctgtaattc gatgtgacta tttcaactgc
     4141 aacaacttca ccggctgaat ttgtgtaaca tccgtcaact gtctggatct ctccggattc
     4201 aattttctct ttgggaattc ccattttttc atactgctca gccatctttc cttcgtgtac
     4261 ccatgtgctc ctttccttat cggaaagcga tatatatttt tcagataatt ttatatcatg
     4321 ggtaagttgt ctcgggttgt atttatagag cttgtcaacc attgccgcct tgacaaattt
     4381 tttcccggtg cgggtcagcc ggacaacagt agtctctttt ccgcttaccc agtatttttt
     4441 attttcgatc attttaagag atttcatatt ctcataagat ttttctgtaa atgccgcttt
     4501 aaaatctgac agtttaatta taccggtatt tgcaagtcca cggaatccgt tcacttcgga
     4561 ttgtgaataa ttaccgacct gaaatttaga ccaagtataa gccatgttac aaaacctcaa
     4621 accgtctgaa cgatagtgaa gtggacgatg caatccgccc taccgacaac atacacggag
     4681 tggatgtttg gcaggtgggg cggattgctt tttcatgtcg gagacccgaa accgtaaggt
     4741 tcggaatctt cttgtatttt atgacatatg tatttaattt ctgtgtctgc atttaataca
     4801 tatccaatac agacaagaat ttttcccgat tactgtattt cttcatcttc atcttttttc
     4861 tttgttgcta atgaaaaatc aacaaatatt ctctctttat attttgggtc tttaacaatt
     4921 gctctgaggt gtttttttat ttcccttacc tgctcgtcgt ttaattcaat gtctttaaaa
     4981 agattgaata ttctttttgc tatatccatg ggggctatta tgtttgtctt ctgttccttc
     5041 tcaagttccc tgtcgtctgt cagttttgcc cgtgctcctt
//
LOCUS       DAOOYB010000203         4988 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_19595_length_4988_cov_5.317048, whole genome shotgun sequence.
ACCESSION   DAOOYB010000203 DAOOYB010000000
VERSION     DAOOYB010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4988
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19595_length_4988_cov_5.317048"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="PL056_10610"
     CDS             complement(<1..386)
                     /locus_tag="PL056_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47507.1"
                     /translation="MRNVLITLLLLLFWSCAACNGNSQNNYSDNILDFDHDSQILPDS
                     HDFDASSNDDITENEQSDSDSDNSYCPDPDTVDFPYYDKNGKMHFCRPCDLENSEFIK
                     KDPSCVSNLWRFENEKKCGLYPKYDC"
     gene            complement(389..4933)
                     /locus_tag="PL056_10615"
     CDS             complement(389..4933)
                     /locus_tag="PL056_10615"
                     /inference="COORDINATES: protein motif:HMM:NF013779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HPM47508.1"
                     /translation="MTKSEIEFSADMILDSVADLSGQNAANFSVYNVETIYYEGAIVG
                     FVINLDPEGFLLVSSADELTPVQLVQFSGTYPDSLPPVFLENIIKENAATMTKLGYYS
                     SLPGIESILSELNGKIKNKVLEERNITLWNLFLNGQNPYFSYLKNQEPNNTTRKSLFT
                     ASWHQSLPYNLYTPIYYGNDPEGERTVAGCTAIAQSILMKYWEWPEYGIGSKSIIFNN
                     GIENETYEADFSTRYRWDLMESAYNSPYNSDATMPDEEDIPESVKQTAKVVFHNGVLV
                     YMDYGVSGSGASFNIKGMVDHFKYSDDFSAFRTGFPSEKIKVIKEQIDNDLPVLADWA
                     GKNDSAGHSFLIIGYDIRDIYLSEINNPDSDYNRKSIFALNLGHGSNQVWKSFEYLYE
                     NNFAGIYIDIYPEGYCGDRDLPYDCDDQDGVPFYKDNCPKHFNPLQEDPDHDGIGLVC
                     QNPEGDYDGDGVKNKNDNCPIVANQGQEDWDNNGVGDACDDFDNDGVVDKDDNCRETY
                     NPLEFFDSAQEFFSMVSTATDYLCSNNGVLNICNSSKRNGLIQAHPEMYKTSGWTHMP
                     PSWQPDHDMDGIGDACDNGEAYNGIFVSVEKTISESENPDSSIILFGKKYKNSYVQLS
                     TWLTRPNATPGSDEVQVSNATFQNFYCAVDQPNLQYWGDAGYCTVTDGENVNRPPAQG
                     GRNFGYSHGTDPYPINPVRNKQAWNETTQSRFLYQITKDNELFCPRESWECNLGLRNP
                     DVAYEGDEGKGMKFLWNWKVDICSDFYTREDLCIPFINPPQGETAAMPFHYTISTGLL
                     AKSRKYLSNLCGNGIVDQGEQCDDGNLLRRDGCDDSCSFEYITKYFVAGETKKFVDPR
                     FFRHEQVYNRSWRMNKNSRRLSYYYTERSLPDISDEIDLPERFDKYLCYKCLIDRIRP
                     GIIDIWRYGDDYASKDRISEPDGRISQVFSTDNSVVWAIEDTGSESILKRYLINASGE
                     WETVSSLLNYPAGLEGSSVIMLEDSFILVGGKNNDAFSPYLYYASQSAESHGFFEIET
                     IAQLPAGVSTAFPMIVNENLYVVGNNGATVSVFALINDDNGNLPFKFEEIALQNKPVA
                     RLTATYENDGSKIFVIGGVDINGNAVNKIEAINLEKSTGWVGMDVENVESMKRGVVLF
                     NGNEITYTDIESANVESTRIHFDIEKMNYKTEIITSIKNGFEAHCLDSISEGTTVTVS
                     DGFLQEEYCNSSLQNYGLIDLPENIRTIEGENNIIYAGSESGIAVINIENIDSPIVME
                     RHSLYGPVKDILIVNGYVYAASGNGIDVFKIENDGKLIKKYHRSTYGDTARLIYFNGR
                     IYAGDGQGIKVISIQNPETPTVIKSVSTSGDISDMALGSSKNLYIYDWGGIKVYNLEN
                     RDNPVKTASKYYSCSNAAMMSYSGGSLMKCGNSLKYLVKNGTSVTIQNISGSPSVKDV
                     FVREGKLFKISNGNIEIMQATGELSAPVCGNGIIESGELCDGNSVACTTISSSYISGS
                     AYCNSTCNGYNEENCETDGW"
BASE COUNT         1464 a         1086 c          801 g         1637 t
ORIGIN      
        1 caacagtcgt acttgggata aagtccgcat ttcttctcgt tttcaaagcg ccacaaattc
       61 gatacacatg aaggatcttt ttttataaat tcactgtttt caagatcgca aggacggcag
      121 aaatgcattt ttccgttttt atcataataa ggaaagtcaa ctgtgtcagg atcaggacag
      181 tagctgttgt ccgaatctga gtccgactgc tcgttttccg taatatcatc atttgaactt
      241 gcgtcaaaat cgtgtgaatc aggcaaaatc tgcgaatcat gatcaaaatc aagtatatta
      301 tcagaataat tattctgact gttcccgttg catgccgcgc atgaccagaa aagcagtaaa
      361 agcaatgtta tcaggacatt acgcatattt accatccgtc cgtctcacag ttctcctcat
      421 tgtatccatt gcatgttgaa ttgcaatatg ctgagccgct tatataagaa gaacttatcg
      481 ttgtacatgc aactgaattt ccgtcacaga gttctcccga ttcaatgatt ccgtttccgc
      541 atactggtgc tgacaactct ccagtcgcct gcatgatttc aatgtttccg ttactgattt
      601 taaatagttt tccttccctc acaaaaacat ctttcacaga tggcgatcct gaaatattct
      661 gaatcgtaac tgaagttccg ttttttacaa ggtattttaa gctgtttccg catttcatga
      721 gtgatccgcc ggaataactc atcattgcag cattgctgca cgagtaatat ttggaagctg
      781 tcttaaccgg attgtccctg ttttccagat tgtacacttt tattccgccc cagtcataaa
      841 tatacagatt tttagaactg cctaaagcca tatcacttat atcgccactg gttgaaacgc
      901 tttttattac agttggtgtt tcagggtttt gaatagaaat aacctttatt ccctgtccgt
      961 caccagcata aattctgccg ttgaaataaa taagtctggc tgtatcacca taagtggatc
     1021 tgtggtattt cttgataagt tttccgtcat tttctatctt gaaaacatca attccgtttc
     1081 cagatgctgc ataaacatag ccatttacta taaggatgtc tttcactgga ccataaagtg
     1141 aatgccgctc catgacaatt ggactatcaa tattttcaat atttataaca gctattccgg
     1201 attcgcttcc ggcataaatt atattgtttt cgccctcaat agttctgatg ttttccggaa
     1261 ggtctatcaa tccataattc tgaagagatg agttacagta ctcttcttgt aaaaatccat
     1321 ctgaaacagt cactgtcgtt ccctctgaaa tagagtcaag acagtgtgct tcaaagccat
     1381 tttttattga tgttataatt tcagttttgt aattcatttt ttcaatatca aagtgtattc
     1441 ttgtgctttc aacatttgca gattcaatat ctgtataggt tatttcgttt ccgttgaaca
     1501 gtacaactcc ccttttcatc gattcaacat tttcgacatc cattccaacc cagccggttg
     1561 atttttcgag gtttattgct tctattttat tgacagcgtt cccgtttata tcaactccgc
     1621 caatcacaaa aatttttgat ccgtcatttt cataggttgc agtcaatctg gcaacaggtt
     1681 tattttgaag cgcaatctct tcaaatttaa acggcagatt gccattatca tcatttatta
     1741 atgcaaaaac agaaaccgtt gcgccattat ttccaacaac ataaagattt tcattaacaa
     1801 tcatcggaaa agctgttgaa actcctgcgg gaagctgtgc aattgtttca atttcaaaaa
     1861 acccatgcga ctctgctgat tgagaagcgt aataaagata tggagaaaat gcatcgttgt
     1921 ttttaccgcc tacaagaata aagctgtctt caagcataat tacagaactt ccctccaagc
     1981 cggcagggta atttaacaaa ctgcttacgg tttcccattc tcccgatgca ttaattaaat
     2041 atctttttaa aattgattca cttccagtat cttcaattgc ccatacaacc gagttatctg
     2101 ttgaaaacac ctgtgaaatt ctaccatcag gttcactgat tctatctttc gaagcatagt
     2161 cgtcaccata tctccaaatg tcaatgattc cgggacggat tctgtcaata agacatttat
     2221 agcacaggta tttatcaaat ctttcgggaa gatcaatttc atctgaaata tccggcaggg
     2281 atctttctgt atagtagtaa gaaagtcttc tgctgttttt attcattctc catgatctgt
     2341 tataaacctg ttcatgacga aaaaatctcg gatctacaaa ttttttcgtt tcaccggcaa
     2401 caaaatattt tgtgatatac tcaaaactgc acgaatcgtc acatccgtct cttcttaaaa
     2461 gattgccatc atcgcattgc tcaccctgat caacgatgcc gtttccacac aaattagata
     2521 aatatttcct tgatttagct aacaaacctg ttgaaatagt atagtgaaag ggcatcgctg
     2581 cagtttctcc ctgaggagga tttataaaag gaatacataa atcttctctg gtgtaaaaat
     2641 cggaacatat atcaactttc caattccaca gaaacttcat tcccttgcct tcatcacctt
     2701 cataagccac atcagggttt cttaaaccta agttgcactc ccaactttct ctcggacaga
     2761 aaagttcatt atcttttgtt atttgatata aaaaacggct ttgagttgtt tcgttccatg
     2821 cctgtttgtt tctcacggga tttatcggat aaggatctgt gccgtggctg tatccgaagt
     2881 tcctgcctcc ctgtgccggt ggtctgttta cattttcacc gtctgtaact gtgcaatacc
     2941 ctgcatcacc ccaatattga agatttggct gatccacagc acagtaaaaa ttctgaaaag
     3001 tcgcattaga cacttgaact tcatcactcc ctggtgtggc attcggtctt gtaagccaag
     3061 tgcttagctg cacatagctg tttttatatt tttttccaaa taaaattatt gaactatccg
     3121 gattctctga ttctgaaatt gttttttcaa ctgatacaaa tattccgtta tatgcctcgc
     3181 cattatcaca cgcatcaccg attccgtcca tatcatgatc cggctgccat gacggtggca
     3241 tgtgtgtcca tccggaagtt ttatacattt caggatgagc ttgaataagt ccgtttcttt
     3301 ttgaactgtt gcaaatattt aagacaccat tatttgaaca aagataatcg gtcgcagttg
     3361 aaaccattga gaaaaactcc tgtgcagaat caaaaaattc aagaggattg taagtttcac
     3421 gacagttgtc atccttgtca acaacgccat cgttatcaaa atcatcacag gcatcgccaa
     3481 ctccgttgtt atcccagtct tcctgcccct gatttgcaac tatcggacag ttgtcatttt
     3541 tgttttttac accgtctccg tcataatcgc cttccggatt ctgacagacc agacctatac
     3601 catcatgatc aggatcttcc tgaagcggat taaaatgttt tggacaattg tctttgtaaa
     3661 atggcacccc atcctgatca tcacaatcat aaggcaaatc tctatcacca cagtaacctt
     3721 ctggataaat atcaatatat atcccggcaa aattattttc gtaaagatat tcgaaagatt
     3781 tccaaacctg attgctgccg tgacctagat tcaaggcgaa aatgcttttt ctattataat
     3841 ctgaatccgg attatttatt tcacttaaat aaatatctct aatatcgtaa cctattatca
     3901 aaaaggaatg cccagcagaa tcatttttcc ctgcccaatc agcaagaacc ggcaaatcat
     3961 tatcaatctg ctctttaata accttaatct tttcgctcgg gaaccctgtt ctgaaagcag
     4021 aaaaatcatc tgaatattta aaatgatcaa ccatgccttt tatattaaaa gatgctcctg
     4081 atccagaaac tccatagtcc atatacacaa gaactccatt gtgaaaaacg acttttgccg
     4141 tctgtttaac agactcagga atatcctctt catccggcat tgttgcatca ctattatatg
     4201 gagaattata tgcactttcc attaaatccc atctgtaacg ggtactaaaa tctgcttcgt
     4261 atgtttcatt ctctattcca ttgttaaaaa ttatgctttt gctcccaata ccatattccg
     4321 gccattccca gtatttcatt agaattgatt gagctattgc cgtacatcct gcaacagttc
     4381 tttctccctc cggatcattc ccataataaa tcggagtgta taaattgtac ggaagggact
     4441 ggtgccatga tgcagtaaac aatgatttcc gtgttgtatt atttggctcc tgatttttta
     4501 aatatgagaa gtaaggattc tgcccattta aaaacaaatt ccatagagta atatttcttt
     4561 cttcaagcac tttgtttttt atttttccgt tcaactccga caaaatgctt tcaatgccgg
     4621 gaagtgaact gtaataacct aactttgtca ttgtcgcagc attttcttta ataatatttt
     4681 caagaaaaac cggcggtaac gaatcaggat aagtcccact aaattgaacc aattgtacag
     4741 gagtaagctc gtcagcagat gaaaccagta aaaatccttc aggatcaaga ttaattacaa
     4801 agccaacgat cgctccttca taataaattg tttcaacatt ataaacactg aaatttgctg
     4861 cgttttgacc actgagatct gcgactgaat caagaatcat gtctgctgaa aactcaatct
     4921 cagattttgt cataatacgg gcgtttaaaa ctgaaaaccc gaaaaaagca atcgccaata
     4981 aaattaac
//
LOCUS       DAOOYB010000204         4964 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_19708_length_4964_cov_9.276635, whole genome shotgun sequence.
ACCESSION   DAOOYB010000204 DAOOYB010000000
VERSION     DAOOYB010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4964
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19708_length_4964_cov_9.276635"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(169..765)
                     /gene="ruvA"
                     /locus_tag="PL056_10620"
     CDS             complement(169..765)
                     /gene="ruvA"
                     /locus_tag="PL056_10620"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HPM47509.1"
                     /translation="MIGYLKGTVVFSQDREIIILTDGGTGYLVSVTPQVASAHSEGDT
                     VELFIETSVREDAIVLFGFPKMEERKLFNLLREVNKIGPKTALSILSSGTAEEIGFAI
                     ASGNAGFFKKISGVGQKTAERIIIDLKDKMGNFPSEGSGTVITDSDRNTLDAQEGLLA
                     LGYNPLQVRHALAKINEKDKMKAEAIVREALKIINGKR"
     gene            complement(769..1248)
                     /gene="ruvC"
                     /locus_tag="PL056_10625"
     CDS             complement(769..1248)
                     /gene="ruvC"
                     /locus_tag="PL056_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HPM47510.1"
                     /translation="MEKKGYIILGIDPGSIKTGFGLINVSGNCYTHIENGLIAPPKGM
                     EFKERVAYIFRGVCETIEEFKPDYLSLEDIFISKNAQSALKLGHIRGAVMSASVVHGI
                     PVFEYSPTRVKQSITGNGRAEKFQIQEMVKLLLKLKEIPQEDAADALAVAITHAFSV"
     gene            complement(1255..2001)
                     /locus_tag="PL056_10630"
     CDS             complement(1255..2001)
                     /locus_tag="PL056_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPM47511.1"
                     /translation="MSGHNKWSTIKHKKGAADAKRGKIFTKIIKEITIAAKAGGGNPD
                     GNPRLKTALLSAKSANMPQDNIIRAIKKGTGELEGITYEEVTYEGYGPGGVAVMVDCT
                     TDNKNRTVAEMRRLFQKAGGNMGESGCVNWMFERKGQIIIPKENITEDQIMNDALEAG
                     AEDVIDSETVWEIRTALSDLYAVSSALSEKGYEVEESKISRIPQNFVKVEGKVAEQAL
                     RLVEMLEESDDVQDVFANYDIDFSTITEEE"
     gene            2172..3518
                     /locus_tag="PL056_10635"
     CDS             2172..3518
                     /locus_tag="PL056_10635"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HPM47512.1"
                     /translation="MRDFKKVLAVFWVMFVFMLDLSASDMHAFEQEVIKISENAKKSV
                     ASIKTERKSRGNVQDPFYDFLRQFGFRGAPQPYNPKSQAMGTGFVIDEKGGYLVTNNH
                     VISKAEKIEITIDGKKFKGKLIGADPQTDIGLVKIEGFKKGDIQELKFANSDNIKVGS
                     FAIAIGNPFGLNHTVTFGIVSAKGRSGMNVTEYEDFIQTDAAINPGNSGGPLINTNGE
                     VIGMNTAIFSQSGGYMGIGFAVPSNMIKEITTQLISGNKIQRAMMGVTIQELNDDLRE
                     YLNIEKNVEGALIGSVAKGSPAEKGGIQSGDVITSFNNKPVDSVSKLKNAVGFSPFGK
                     KLPVEIIRNGKNMKLEVVLEKEFLMSETESFSSEEVAGELGLTLTTDKNNVTVSKVEN
                     GSLAFMAGIMEGDIIIAVNKKKVKNVSEVVNIVNKSKSILFLINRKGREFFVVVNR"
     gene            3598..>4964
                     /locus_tag="PL056_10640"
     CDS             3598..>4964
                     /locus_tag="PL056_10640"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HPM47513.1"
                     /translation="MRNLLPDLKQLGYFKNNVTNSSGCIDASVLFVDVSGFTNMTRKL
                     MEQGKDGAEILSEFMNTIFNPLIEAVYSYNGFIINFAGDAFTAVFKDDDGRRAVATAI
                     DIRTFFIGNPVLSHKELSFKISAKSGISSGKLCWIAAKGGDIEHFLFFGDVVDKAAEA
                     EHCCTGNDICVNSIVYEMIHDSVESVFNGVSYFVEELKIQIEKEHSESTLAIEDLKKF
                     IPQSVLEQRIAGEFREVVSLFISYKMPENNKDIENMLSYLAGFVQKYGGYISGFDFGD
                     KGATLLALFGAPVSFEDNLERAVNCVSDIRKNISTEMRSGITEGVVYAGFTGSSRRSA
                     YTALGDNVNLAARLMMKAPFGSDWIAGKAASRAKSGYNLESSGFFKFKGMDCDIEVFE
                     IKGKLNKEEGSSFSTAFTGRSMELAELIEMAGMAKELKKLHSYTIFGEAGIGKSRLIY
                     EVKRKA"
BASE COUNT         1402 a         1026 c         1093 g         1443 t
ORIGIN      
        1 gaaggtggtc aagctggcgg tcatttcttt ttgccgcctc gatatatatc tttatgttgt
       61 cgataactgt tttctgtccg atgtagccgg aaaggttctg cggtcgcaaa ccgccttcaa
      121 gggaagagtc ttcaaatttg acttccgagc ctaccagtct gtcttccatc atcttttacc
      181 gttaattatc tttaatgcct cacgaactat cgcttcggct ttcatcttat ctttttcatt
      241 tattttggca agtgcatgtc ttacctgaag agggttgtat ccaagagcta aaaggccttc
      301 ctgtgcatcg agagtgtttc tgtctgaatc agtaatgacc gttccgctcc cttcggaagg
      361 aaagttgccc attttgtctt tcagatctat aattatcctt tctgcagttt tttggcctac
      421 accgctgatc ttcttaaaga atccggcgtt ccccgatgca atggcaaacc cgatctcttc
      481 cgctgttccg cttgaaagga tggagagagc ggtcttgggt ccgatcttgt taacttcccg
      541 aagaagatta aagagcttcc tctcttccat tttaggaaat ccgaacagaa cgatcgcatc
      601 ttctcggacc gaagtttcga taaaaagctc aacagtgtct ccctcagagt gagcagaagc
      661 tacctgaggt gtaacactta ccagataacc ggttcctccg tcagtaagga ttattatttc
      721 ccgatcctgc gaaaaaacca ctgtcccttt taaataaccg atcatttttt acaccgaaaa
      781 agcatgtgtt attgcgaccg caagtgcatc tgcggcatct tcctgaggta tttcctttaa
      841 tttaagaagg agcttcacca tttcctgtat ctgaaatttc tcagcccttc cattccctgt
      901 tatcgactgt ttcactcttg taggcgaata ctcgaacacc ggtattccgt gtactacaga
      961 agcgctcatg acagcacccc tgatgtggcc cagtttcaaa gcactctgag cgttctttga
     1021 aatgaagatg tcttcaagag aaagataatc gggtttgaat tcctcgatag tttcacaaac
     1081 tcctctgaaa atgtatgcga ccctttcctt gaattccatc cctttgggag gagcgatcag
     1141 cccgttttca atgtgagtat agcagttccc tgaaacattt atcagtccga aacctgtttt
     1201 gatactgccg ggatctattc ctaagataat gtatcctttt ttttccaaaa ggggctattc
     1261 ctcctctgtt attgtggaga aatcgatatc gtaatttgca aagacatcct gaacatcgtc
     1321 gctttcttca agcatctcaa ccagacggag agcctgttca gcgacttttc cttcaacttt
     1381 gacaaagttc tgaggtattc ttgatatttt gctttcctct acttcatatc ctttttcaga
     1441 aagtgcggaa gatactgcat acagatcgga aagggctgtc ctgatctccc agacagtttc
     1501 gctatcgatc acatcttcag cacctgcttc aagggcgtcg ttcatgatct ggtcttcggt
     1561 gatgttctct ttggggataa ttatctgacc ttttctttca aacatccagt tgacacaacc
     1621 gctttcaccc atgttgccgc cggctttctg gaaaaggcgc ctcatctcgg ctacagttct
     1681 gttcttgttg tctgtggtgc agtcaaccat tactgcaact ccgcccggtc cgtatccttc
     1741 gtatgtcact tcttcgtagg tgatcccttc aagttcaccg gtaccttttt tgatggcacg
     1801 gatgatgttg tcctgcggca tgtttgcact tttggctgaa agcagggcag ttttgagtcg
     1861 cgggttgccg tcgggatttc cgcctccggc ctttgcagca atggttattt ccttgatgat
     1921 ctttgtgaaa atcttgcctc ttttagcatc tgctgcacct tttttgtgtt taatggtgct
     1981 ccacttgtta tgacctgaca tcaaaccctc cggataaaaa aaagttacat acaataaaaa
     2041 accagatttc ttaccataaa tcatattctt tggcaagttt ttaaaatggt ttcagccctt
     2101 gaattttcac aaaactgaaa taaaaatata atggcgattg ttgagtatgt ggattgacca
     2161 tggaggttga aatgagagat tttaaaaagg tgctggcggt tttttgggtt atgttcgtgt
     2221 ttatgctgga tctgagtgct tccgacatgc atgcttttga acaggaagtg atcaaaatat
     2281 ctgaaaatgc gaagaaaagt gtcgcttcga taaaaactga aagaaaatcc agaggcaatg
     2341 ttcaggaccc tttttacgat tttttaaggc agttcggttt caggggcgct cctcagccgt
     2401 ataatcccaa aagtcaggcg atgggaacag gttttgtaat tgatgagaaa gggggatatc
     2461 ttgttacaaa taaccatgta atttcaaaag ctgagaagat agagataaca atagacggca
     2521 agaagttcaa ggggaagctt atcggtgccg atccacagac agatatcggg ctggtaaaga
     2581 tagaaggttt caaaaaaggg gatatccagg agcttaaatt cgcaaattca gacaatataa
     2641 aagtgggcag ttttgccatc gcgataggca atcctttcgg gctgaaccac actgtaacat
     2701 ttggaattgt ttcggcaaaa gggcgttcag ggatgaatgt gacagaatat gaggatttca
     2761 ttcagacaga tgcggcgatc aatcccggca actcaggcgg tcctctcatc aacacaaacg
     2821 gtgaagttat cgggatgaac actgcaattt tttcccagtc aggcggatat atggggatag
     2881 gttttgcagt tccttccaat atgatcaaag agataactac tcaacttatt tcaggaaaca
     2941 agatacagcg cgccatgatg ggggtgacta ttcaggagct caatgacgat cttcgtgaat
     3001 atctcaacat tgaaaagaat gtggagggag cgctgatagg cagtgtcgcc aagggcagtc
     3061 ccgccgaaaa gggaggaata cagtcaggag atgtcataac gagtttcaac aacaagccgg
     3121 tggacagtgt ttcaaaactt aaaaatgctg tcggtttcag cccgtttggt aaaaaacttc
     3181 cggttgaaat aatcaggaac ggcaaaaaca tgaaactcga agtcgttctt gaaaaagagt
     3241 ttctgatgtc tgaaaccgaa tctttctcat ctgaggaagt tgcaggagag ctcggactta
     3301 ctctcactac ggacaagaac aatgtgaccg tttcaaaagt tgagaacgga tctcttgctt
     3361 tcatggccgg catcatggaa ggggacatca tcatcgctgt caacaagaaa aaggtgaaaa
     3421 atgtgtctga agtggtcaat atcgtcaata aatccaaatc tatacttttt ctcatcaaca
     3481 gaaaggggag agagttcttt gttgtcgtga acaggtaatg ttccagacac cttattaaac
     3541 ttgttttatt ttctgccttt tctataatat tttgttactg atttttacag gaataaaatg
     3601 agaaaccttc tacccgacct taaacaactt ggttacttca aaaataatgt aacaaattca
     3661 tcaggatgta tagatgcatc agttctcttt gtagatgttt ccggctttac aaacatgaca
     3721 agaaagctta tggaacaggg aaaagacggt gctgaaatat tatccgagtt catgaacaca
     3781 atattcaatc ctctcatcga agcggtttac agttacaacg gattcataat aaattttgcg
     3841 ggagatgctt tcaccgcagt atttaaggat gacgatggaa gaagggctgt tgcgacagcg
     3901 attgacatta gaaccttctt tatcggaaat ccggtgcttt cccacaagga actttctttc
     3961 aaaatatcag caaaatcagg tatttcttcg ggcaaactgt gctggatagc cgcaaagggc
     4021 ggagacattg agcatttcct tttcttcgga gatgtcgttg acaaggcagc cgaggcggaa
     4081 cattgctgca cagggaatga catttgtgtg aacagcatag tctatgaaat gatccatgat
     4141 tctgtcgaaa gtgtattcaa cggagtgtct tattttgtgg aagagctgaa aattcagata
     4201 gaaaaagagc attccgaaag tactctagcc attgaggatc ttaaaaaatt cattcctcaa
     4261 tctgttcttg agcagagaat agcaggcgag ttccgcgaag tggtctccct tttcatttca
     4321 tataaaatgc ctgaaaacaa caaagatatt gaaaatatgc tgagctatct tgccggattt
     4381 gttcagaaat atggcggata tataagcggc tttgatttcg gagataaagg ggcgacactt
     4441 cttgcccttt tcggagcacc tgtcagtttt gaagataacc ttgaacgggc ggttaactgt
     4501 gtcagtgata tcagaaaaaa tatctcaact gaaatgaggt cgggaataac tgaaggagtc
     4561 gtttacgccg ggttcaccgg aagttcaaga agatcggctt ataccgctct gggagacaat
     4621 gtcaatcttg cggcaagact catgatgaaa gctcctttcg gctcagactg gatagccggc
     4681 aaggcagcat caagagcaaa gagcggatac aatcttgaat catcgggatt tttcaagttc
     4741 aaagggatgg actgtgacat tgaagtcttt gaaataaagg gaaaactcaa taaagaagag
     4801 ggctcttcat tttccacagc tttcacagga agatcaatgg agcttgcaga acttattgaa
     4861 atggcaggaa tggcaaaaga acttaaaaaa cttcattcat acaccatatt cggtgaagca
     4921 ggcatcggaa aatctcgact tatctatgaa gtaaaaagaa aagc
//
LOCUS       DAOOYB010000205         4963 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_19715_length_4963_cov_8.181744, whole genome shotgun sequence.
ACCESSION   DAOOYB010000205 DAOOYB010000000
VERSION     DAOOYB010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4963
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19715_length_4963_cov_8.181744"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..967
                     /locus_tag="PL056_10645"
     CDS             <1..967
                     /locus_tag="PL056_10645"
                     /inference="COORDINATES: protein motif:HMM:NF020672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SelB C-terminal domain-containing protein"
                     /protein_id="HPM47514.1"
                     /translation="EPCVLKPGDRFVIRNTSGDMTLGGGEIIDVSPLHHKRRKSKIIN
                     DVGKLASGSVAEKIAVHIGKFNRAVSTSQLGFMLNISDDEINDLVSRKLPNEIVRFET
                     KEGFILHTKTGMDNVRKGILKAIADFKKKNPLASRGANFDEIRGFLKFEKNSPDEEIL
                     RQVLNNLTEKEKLKEKEKTWTLANDMTEFDAKTVSNINDISKYTLDCGMQVPLMSQML
                     EMAQKMKITEKALKQILHHLTVTGVLYRISEEYVHKDVVDGCRNKLVDYLSTHNEGIT
                     VAQFRDLVGGNRKMCLLLISQYDSEKITVREGDYRFLNPKFEGVK"
     gene            967..1584
                     /gene="yedF"
                     /locus_tag="PL056_10650"
     CDS             967..1584
                     /gene="yedF"
                     /locus_tag="PL056_10650"
                     /inference="COORDINATES: protein motif:HMM:TIGR03527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HPM47515.1"
                     /translation="MDSVIVDARGELCPKPLIMAKKAINDEKIVNEFILLIDNETSKE
                     NVERFLKDNKVVFQTTKKDECFSIYILKSGEKLSGKVDEYCDLPEEKKPSSDHVVCIK
                     SDKMGIGDDDLGAILIKGFINTLKEVNPLPSKLVFYNSGVKLAVEDSPLADSIRELQD
                     LGVEIMICGTCADFFKIKEKIKTGTISNMYNILETLTSAGKIIVP"
     gene            1581..2708
                     /locus_tag="PL056_10655"
     CDS             1581..2708
                     /locus_tag="PL056_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016778572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPM47516.1"
                     /translation="MREINKYFDNGATSFPKPPEVAEEIAGYLNEIGGTYGRSYHSKA
                     IQVSKIVEETRNMFAKMINAHNSSNIVFTHNATHGINTVLKGLNLKNCEVAVSVLEHN
                     AVMRPLTAIAEQNSIKIRFIPSFSDGLIDVSKVSEVITEKTALVVVNHMSNVNGVIQP
                     VEEIKEIIGDKPILVDAAQSGGHIKIDVLKGLDFVAFTGHKGLLGPTGTGILYIRNPE
                     MLDSFIEGGTGSRSESVEMPQFMPDKFEAGTPNIAGIFGLRAALMNRPVPAHSFKELA
                     QLIENIEEIDGFNVVRANDINNQGALFSILSSVMDPSVLGMKLFEKYSIETRIGLHCA
                     PLAHKHLGTFPQGTVRISFSPYHTNEDIEYLINSLREISKL"
     gene            2705..2923
                     /locus_tag="PL056_10660"
     CDS             2705..2923
                     /locus_tag="PL056_10660"
                     /inference="COORDINATES: protein motif:HMM:NF023251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HPM47517.1"
                     /translation="MILFLFENTRSVIVAEEEILKKKVKCRIIPVPRSISSQCGLAIE
                     AELDSEIQISGILTEKQIKFDIYREYKK"
     gene            2927..3994
                     /gene="selD"
                     /locus_tag="PL056_10665"
     CDS             2927..3994
                     /gene="selD"
                     /locus_tag="PL056_10665"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HPM47518.1"
                     /translation="MMKKPFDLLSTVEYGGCSAKLPPQVLEAILKEIQFDRPQQLLVG
                     AETSDDAAVWKIDENSAIIHTTDFFPPICSDPYEFGAIAAANALSDIFAMGGKPLTAL
                     NLVMFPSTKIDISVLKEILKGGADKAKEAGAVICGGHTIDDYPPKYGLAVTGIVHPDK
                     IIRNCSARAGDVLILTKPVGTGAIVAGQRIGMCSREDYQSALDTMKQLNRAGAEIMQK
                     YNVKCATDITGFSVLGHALEMAKGSGVSIEIDASKVPQLCGAYELIDAGCIPGASFRN
                     LKFVEEHVEFADDLDYNLKMLMLDPQTSGGLLISCTRDISEHILKDLSDSGYHKSSIV
                     GRVLKNNSNNKLLRVILNSSV"
     gene            4144..4953
                     /locus_tag="PL056_10670"
     CDS             4144..4953
                     /locus_tag="PL056_10670"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPM47519.1"
                     /translation="MSKSVGNRWLIAIMGTTLQLALGTVYAWSYFQKPIMTSFGWSNV
                     TVMWIFSLSICFLGLSAAWGGIKLAKFGPRKLAVTGAILWASGWLIGALAMSMQSVVL
                     LYIGYGVIGGCGLGLGYVTPVATAAKWFPDKKGFITGMVVMGFGLGALIMSKLIAPGL
                     MADFDSILKNIPAVAYVDGVLVDEAIKASLQGQLVHVFMYAGIIMFCIAFPAAMVLKN
                     PPAGYVPEGYTPPVKSAAAQGGEDKLTVKACITSSKFFIEVPPKKIQQISN"
BASE COUNT         1620 a          880 c         1120 g         1343 t
ORIGIN      
        1 cgaaccgtgc gttcttaagc ccggagacag atttgtcatc aggaacactt ccggcgatat
       61 gactttaggc ggtggcgaga taattgatgt ctcaccgctt catcacaaaa gaagaaaatc
      121 taaaataatc aatgatgtag ggaagttggc aagcggcagt gttgccgaaa aaattgcagt
      181 gcacatcgga aagttcaaca gagccgtatc aacttcgcag cttggtttca tgctcaatat
      241 ttcagatgat gagatcaatg atctggtgag tagaaaactt ccaaatgaaa ttgtgagatt
      301 tgaaacaaaa gaaggattca ttcttcacac aaagaccggc atggacaatg tcagaaaagg
      361 gatattgaaa gcgatagctg atttcaaaaa gaaaaatccg cttgcctcaa gaggggcgaa
      421 ctttgatgag atcagaggat ttctaaagtt tgaaaagaac tctcctgatg aagagatcct
      481 gcgacaggtt ttaaataatc tgacagaaaa agaaaagctc aaagaaaaag aaaagacatg
      541 gacacttgcc aatgacatga ctgaatttga tgcaaaaaca gtcagcaata ttaatgatat
      601 atcaaaatat acattagatt gcggaatgca ggttccctta atgagccaaa tgcttgaaat
      661 ggcgcaaaaa atgaaaataa ctgaaaaagc gttaaaacag atacttcatc atctcacagt
      721 tacaggagtt ttatacagaa tatctgaaga atatgtccat aaagatgtcg ttgacggctg
      781 cagaaacaag cttgttgact atctttcaac ccataatgaa ggaattaccg ttgcacagtt
      841 ccgcgatctt gtaggaggca atagaaagat gtgtcttctg ctgatatccc agtacgacag
      901 cgagaaaatt acggtcagag aaggtgatta cagattttta aacccaaaat ttgaaggagt
      961 gaaataatgg attctgttat agttgatgca agaggcgagc tttgtcccaa gccgctcata
     1021 atggcaaaaa aagcgataaa tgatgaaaaa attgtaaatg aattcatcct tctgatagat
     1081 aacgaaacat caaaggaaaa tgttgaaagg tttttgaaag ataacaaagt cgtttttcag
     1141 acaacaaaga aagatgaatg tttttcaata tatatcttaa aatccggaga aaagctaagt
     1201 ggcaaagttg atgaatattg cgatctgcca gaagaaaaga aaccttcatc agatcatgtg
     1261 gtctgcataa aaagtgataa aatgggaatt ggcgatgacg atctcggggc aatacttatt
     1321 aagggtttta taaatacatt gaaagaagta aacccgctcc cttcaaaact ggttttttac
     1381 aacagcggag tgaaacttgc cgttgaagat tcgcctcttg ccgattcgat cagagaactt
     1441 caggatttgg gtgtcgagat catgatctgc ggaacctgcg ccgatttttt caaaattaag
     1501 gaaaagataa aaacaggaac aatttcaaat atgtataata ttcttgaaac attaaccagt
     1561 gccgggaaaa taatagtgcc atgagagaga taaataaata cttcgataac ggggcgacct
     1621 ctttcccgaa gccaccggaa gttgcagaag agattgcagg atatttaaat gagattggcg
     1681 ggacttacgg tcggagttat cattctaaag cgattcaagt ttctaaaatt gttgaagaaa
     1741 caaggaacat gtttgctaag atgatcaacg ctcacaattc ttcaaacatt gtctttactc
     1801 acaacgcaac tcacggaata aacacagtgc ttaaaggtct caatttaaaa aactgtgaag
     1861 ttgcagtttc agttcttgaa cataatgcgg taatgagacc tctgacagca atcgctgagc
     1921 agaacagcat aaaaataaga tttatccctt cattttctga cggtcttatt gatgtttcaa
     1981 aagtttctga agtgataaca gaaaaaacgg cgcttgtagt tgtcaaccac atgagcaatg
     2041 tgaacggagt gatccagccg gttgaagaaa taaaagaaat tattggcgac aaaccgatac
     2101 ttgttgatgc cgctcaaagc ggaggtcaca taaaaataga tgttttaaaa gggcttgatt
     2161 ttgttgcttt taccggccac aaagggcttc tcggcccgac cgggaccgga attctttata
     2221 tcagaaaccc tgaaatgctt gattcattca ttgaaggggg aacgggaagc agatctgaaa
     2281 gtgttgaaat gccgcagttc atgcctgata aatttgaagc aggtactccc aatatcgcag
     2341 gaattttcgg attgagagcg gctttgatga acagacctgt tccggctcat tcattcaaag
     2401 agcttgctca actgattgaa aatattgaag agatagatgg gtttaatgtt gttcgtgcaa
     2461 atgatataaa caatcaggga gcgctttttt ccattttgag cagtgtcatg gatccctcag
     2521 ttcttgggat gaaactgttt gaaaaataca gcattgaaac aaggatagga cttcactgtg
     2581 caccgttggc gcataagcat cttggaactt ttccgcaagg gacagtaaga atcagttttt
     2641 ccccttacca cacgaatgaa gatattgaat atttgataaa ttcattaaga gagatctcta
     2701 aattatgata ctttttcttt ttgaaaacac ccgctcagtc attgttgctg aagaagagat
     2761 attaaagaaa aaggtaaaat gcaggataat tccagtaccg agatcaatat catcccagtg
     2821 cggacttgct attgaagcag aacttgacag tgaaatccag atttcgggaa ttttgacaga
     2881 aaaacaaatt aaattcgata tttacagaga gtataaaaaa tgaggaatta tgaaaaaacc
     2941 gtttgacctt ctctcaaccg ttgaatacgg cggatgttca gcgaaacttc caccccaggt
     3001 acttgaagca atattaaagg aaattcagtt tgacagacct cagcaactgc ttgtcggcgc
     3061 tgaaacaagt gacgatgcgg cggtttggaa aattgatgaa aacagcgcga tcatccacac
     3121 aactgatttc tttcctccaa tatgctcaga cccatacgaa tttggtgcta tcgccgcggc
     3181 aaacgcatta agcgatatct ttgcaatggg tggaaagccg cttactgcac ttaatcttgt
     3241 aatgttcccg tcaacaaaaa ttgatatttc ggtgttaaag gaaattctta aaggcggtgc
     3301 ggataaagca aaagaagcag gtgctgtcat ctgcggcggt cacacgatag atgactatcc
     3361 tccaaaatac ggacttgcag ttacaggaat agttcatccg gataaaataa taagaaattg
     3421 cagtgccaga gcaggtgatg ttctgattct cacaaaaccg gtgggaacag gagcgatcgt
     3481 tgccgggcag agaataggga tgtgttcaag agaggattat cagtcagcac ttgacacaat
     3541 gaagcagttg aacagagccg gtgctgaaat aatgcagaaa tacaatgtga aatgtgcaac
     3601 ggacattacc ggcttcagcg ttttagggca cgcgcttgaa atggcaaagg gtagtggagt
     3661 atcaatagaa attgatgctt caaaagttcc tcagctttgc ggagcttacg aacttattga
     3721 tgccggctgc attcccggtg catcgttcag aaatctgaaa tttgttgaag aacatgttga
     3781 atttgcagat gacctcgatt acaaccttaa aatgctcatg ctcgatcctc agacttcggg
     3841 tggcttgctc ataagctgca caagagatat ttccgaacat atccttaaag atctctcaga
     3901 ttcgggatat cataaaagtt ctatagtggg cagagtgctg aagaacaata gtaataacaa
     3961 gttgttgcgt gtgattctga actcgagtgt ctaaaaatct caagaaaatg atcaaaaaat
     4021 ttgacaatcg ctcttcaagt agattaaagt cgctcggcta ggcgagaatt ttttaaaaaa
     4081 aaggatatgt ttttgatttt tttgagagta tagctggtcg ttaatttttt tatggagtta
     4141 aaaatgtcga aaagtgttgg taacagatgg cttatagcta tcatgggaac aactttgcag
     4201 ttggctctcg gtacagttta tgcatggagt tattttcaga agccgatcat gacatcgttc
     4261 ggctggtcaa atgttacagt aatgtggata ttcagcctgt caatctgctt tctgggttta
     4321 tcagcagctt ggggcggaat taaacttgca aaattcggac cgagaaagct ggcagtaacc
     4381 ggggccattc tttgggcaag cggttggttg ataggtgctc tggcgatgtc tatgcagagt
     4441 gtcgttcttc tgtatatcgg ttatggtgtg attggcggat gcggacttgg gctcggatat
     4501 gtaactccgg tcgctaccgc agcaaaatgg ttcccggata aaaaaggatt cataacagga
     4561 atggtcgtaa tgggtttcgg acttggcgct cttattatgt caaaactgat cgcccccggg
     4621 cttatggctg attttgatag tatccttaaa aatattcctg cagtagcata tgttgacggt
     4681 gtgttggtag atgaagctat aaaagcatct cttcaaggtc agcttgtgca tgtttttatg
     4741 tacgcaggta taattatgtt ctgtatcgct ttccctgcag cgatggtatt gaaaaatcct
     4801 cctgcaggat atgttcctga agggtatact cctcctgtaa aaagcgctgc agctcaaggc
     4861 ggagaagaca aacttaccgt aaaagcttgt attacttcaa gcaagttctt cattgaagtt
     4921 cccccaaaaa aaattcagca gatttctaac tgaagaggag gag
//
LOCUS       DAOOYB010000206         4951 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_19776_length_4951_cov_9.376021, whole genome shotgun sequence.
ACCESSION   DAOOYB010000206 DAOOYB010000000
VERSION     DAOOYB010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4951
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19776_length_4951_cov_9.376021"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..1224)
                     /locus_tag="PL056_10675"
     CDS             complement(61..1224)
                     /locus_tag="PL056_10675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47520.1"
                     /translation="MFSKFNLLTLFFGVSALVCISCDGGSSSSSDVKQAYGEPTKNVV
                     TRMRVKQSDGTYGYRLGRITGEQTINDVVYSTYQLTKIDVNGYPIAGSNAMDFYATPL
                     PMDSNGKITIAGVDDHDGLDLTITPVVTLDPNSPVGVAQTVSTSVSGTAPGGVVITNA
                     TANGSYTLVSTGESVSTDMGVVPDCNHFNATVTIDGEGAPEIFKGKTLTGDLWHHPSM
                     GIVKYSIPDLGIEGDIQGTWDVDDPTGEYRTIKMTGVVNSENPAFKLSTYDVSGVYDA
                     DKNTHAKMLVELRWADEETAKTGTMPSYPFVDLSFTTMIGIFSYDLIESPVSIFFPEE
                     NGKGFKYWYAYVNQAAKNEMGGNGICYVINVTADPSASPLRVTGRIHYRTTTE"
     gene            complement(1249..1914)
                     /locus_tag="PL056_10680"
     CDS             complement(1249..1914)
                     /locus_tag="PL056_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine pyrophosphorylase"
                     /protein_id="HPM47521.1"
                     /translation="MEELKITSLLDLSQTEFADIFESCEYPFEILKKLGSYIIEKGPR
                     LDSRYKLIKENVWVGEGTTIADSCSIAGPAIIGCNCEIRHNAFIRGNAVIGNKCVIGN
                     STEVKNSFLFNSVQAPHFNYVGDSVMGYKSHIGAGVILSNVKSVPGTVSIRTAEEIIE
                     TGLRKFSSVLGDHVEVGCNSVLNPGTIVGKGTVIYPLTSARGFIPENHIVKNDGRKFS
                     KRI"
     gene            complement(1924..2577)
                     /locus_tag="PL056_10685"
     CDS             complement(1924..2577)
                     /locus_tag="PL056_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012673729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HPM47522.1"
                     /translation="MEQNKIFYVAIAAVPVLFSIIFHEVAHGWMANRLGDSTAKEHGR
                     LTLNPLAHIDPVGTILVPIVTYFAFGFFFGSAKPVPFNPYRFYNHINMRKGTMWVAAA
                     GPLSNLLLAVISAFAYIATAKFFPNETLLTFFYIALFFNVLLGFFNMIPIPPLDGSKV
                     FMGFLPREYDAFFYKIERYGFFIIIALAVTGAFRFIMMPVNWIVEGLLYIPNLIFFG"
     gene            complement(2578..3498)
                     /gene="xerD"
                     /locus_tag="PL056_10690"
     CDS             complement(2578..3498)
                     /gene="xerD"
                     /locus_tag="PL056_10690"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HPM47523.1"
                     /translation="MIGSDGRLLKKYLNFVKLEKRLALNSVEAYERDLTDFLNYLEER
                     KTSLTTADPVNISEYVIHLSKDKNFSDKSLARSISSMRGFFKFLVEDDFVSPELFEIL
                     EPVKMKKSLPMFLTLDEVDLLFSVIDTSTPSGFRDRTILEMFYSSGLRVSELTELKLG
                     SIFRKDQVILVFGKGSKERVVPYSDNAAEYLHHYLDRLRHSLIKPGDFSDFVFINNRG
                     KKLTRQGVWKKLKEFAYMAGITKEISPHKLRHTFATHLLEGGADLRSVQMLLGHASIN
                     TTEIYTHVEQQAIKREFEQKHPRENMDKEE"
     gene            complement(3511..4329)
                     /gene="asd"
                     /locus_tag="PL056_10695"
     CDS             complement(3511..4329)
                     /gene="asd"
                     /locus_tag="PL056_10695"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00163.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HPM47524.1"
                     /translation="MSYLKLLFLFIISLPFLSRAWGAIAKIETPQWIVKKVIELYRDH
                     YGIDMKDYEGDVGDYNSLSSFFVRKLDSDVRPLVNDENAFLSPSDGKITVLEKINADI
                     ATQVKGRTYPISELIRKELDFPDGYYLVTIYLSPKDYHRYHVPADSVVKSYIHTGWRL
                     FPVNELSVNTIEKLFIRNERVVVKMEHDGMQYFYVAVGASFVGSLKMDFFEEPVDGEW
                     TLVNKKYKQNQELGRFEMGSTIVLLIPEKMVGEPMIAQGVSVKTGQPLFMLKKD"
     gene            complement(4339..4482)
                     /locus_tag="PL056_10700"
     CDS             complement(4339..4482)
                     /locus_tag="PL056_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47525.1"
                     /translation="MKRNLFITTAVAAFIALIMWISGISGPFYRFNRDIINSLTDLIL
                     SIF"
     gene            complement(4479..>4951)
                     /locus_tag="PL056_10705"
     CDS             complement(4479..>4951)
                     /locus_tag="PL056_10705"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF024897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HPM47526.1"
                     /translation="PGDPRDVYKKQVATLTHNDSAKYEDRWVRIKANPTNKSPFLRAI
                     DSFDVAIRHGEGKFVAHRHFLREIRNDDLIAFQYVDKNGEPTRNYPENPNGSDLAIAG
                     ITNSRGNVLGLMPHPEVFINGTQHPEWTRKGTGGEKGMGLQIFVNAVNFVKEEL"
BASE COUNT         1374 a         1120 c          981 g         1476 t
ORIGIN      
        1 tattgtggct tcagatcctt tttggaaact tggaaaactg aaaattattt caaacctgaa
       61 ttattcagtg gttgtacggt agtgaattct tcccgttacg cggagaggag atgctgacgg
      121 gtcggctgtt acattgatca cataacagat gccgttacca cccatttcat tttttgccgc
      181 ctgattgaca taggcatacc agtatttaaa acctttgcca ttttcctcgg ggaagaatat
      241 tgaaaccggt gattcgatga gatcgtatga aaaaatgcct atcattgtag tgaagctcag
      301 atctacaaaa ggatacgacg gcattgtgcc tgtttttgca gtttcttcat cagcccaacg
      361 caattcaacc agcatttttg catgagtgtt cttgtcggca tcataaacac ccgagacatc
      421 atatgttgaa agtttgaatg ccggattttc actgttcaca acaccggtca tttttattgt
      481 gcggtattca cctgtgggat cgtccacatc ccatgttccc tgtatatcgc cttctattcc
      541 cagatcgggt atcgagtatt tcacaatacc catcgacgga tgatgccaga gatctcctgt
      601 aagtgttttc cctttgaata tttcgggagc tccttctccg tcaattgtca cagttgcatt
      661 gaaatggttg cagtcaggaa ctactcccat atcggtagaa acgctttcgc ctgttgaaac
      721 aagagtatat gagccgtttg ctgtcgcatt tgtgatcaca acacctccgg gggcagtgcc
      781 tgaaacgctt gttgacaccg tctgggcaac gcctacaggt gaattcggat caagggtgac
      841 gacaggagtt atcgtcagat ccaaaccgtc atgatcgtca actcccgcaa tagttatttt
      901 accgttactg tccattggaa gaggtgtcgc ataaaaatcc attgcgttac ttccggcaat
      961 cggatatccg ttgacatcta tttttgtcag ctgataagtg ctgtaaacaa catcgttgat
     1021 cgtctgttct ccggtgattc tgccgagccg gtaaccataa gtgccgtcag actgcttgac
     1081 cctcattctg gtgacaacat tttttgtcgg ttctccgtag gcctgtttca catctgaact
     1141 tgaagaactg cctccgtcac aggaaatgca aactagtgca ctcacaccaa aaaacagagt
     1201 caataaatta aatttgctaa acatttcaaa ctccatagtt acaatctgtc agatcctttt
     1261 agaaaacttt cgcccgtcat tttttacaat atgattttcc ggaatgaaac ctctggcact
     1321 tgtgagagga taaatgactg ttccttttcc gactatagtt ccgggattga gaactgaatt
     1381 acagccgacc tcaacatgat cgccgagaac agaggagaat ttgcggagtc cggtttcaat
     1441 tatctcttct gctgtcctta tcgatacagt tcccgggaca cttttaacat tggacaagat
     1501 aacgcctgct ccgatgtggg atttataacc cataaccgaa tcacctacat agttgaaatg
     1561 aggtgcctga acgctgttga aaagaaagct gttcttcact tcggttgagt tgccgatcac
     1621 acatttattt ccgatgactg cattacctct gataaaagcg ttgtgcctta tttcgcagtt
     1681 acaaccgatt atcgccggac cggcaatcga gcaagaatca gcgattgtcg tcccctcgcc
     1741 tacccataca ttttccttta tcagcttgta tcttgaatcg agccgtggcc ctttttcaat
     1801 gatgtaactg cctaatttct ttagaatttc aaacggatac tcacaagatt caaaaatatc
     1861 tgcaaactca gtttgagaaa gatctaaaag tgaagtgatc ttaagttctt ccatggctta
     1921 ctcctatccg aaaaaaataa gattcggaat atataaaagt ccttcaacga tccagttcac
     1981 aggcatcatt ataaatctga aagcaccggt cacagcaaga gcgatgatta taaaaaagcc
     2041 gtatctttca attttataga agaatgcatc atattccctc ggaaggaaac ccatgaaaac
     2101 cttgcttccg tcaagaggag ggatcggtat catgttgaaa aaaccgagaa gaacattgaa
     2161 gaaaagtgca atgtagaaaa atgtaagcag tgtctcgttc gggaagaact ttgccgttgc
     2221 gatatatgca aaagcgctga tcacagcaag cagaagattg ctcagaggac ctgccgccgc
     2281 aacccacatc gtcccttttc tcatgttgat gtggttatag aaacggtaag gattgaacgg
     2341 aaccggtttt gcactgccga aaaagaaacc gaaagcaaaa taagttacta tcggaacaag
     2401 tatggttccg acaggatcaa tgtgtgcaag cggattgaga gtgagccggc catgttcctt
     2461 tgctgtcgaa tcaccaagac ggtttgccat ccagccgtgt gcaacttcat gaaaaattat
     2521 tgaaaaaaga accggcacag cggcgatcgc cacataaaat atcttgttct gctccattta
     2581 ttcctcctta tccatatttt ctcttggatg tttctgttca aattcccttt ttatcgcctg
     2641 ctgttcaaca tgagtgtaga tctccgtagt attaatgctt gcgtgtccga gaagcatctg
     2701 gacacttctg agatcagctc ctccttccag aagatgggtc gcaaaagtgt gccttaactt
     2761 atgaggcgat atctctttcg taatccctgc catatacgcg aattccttca actttttcca
     2821 gacaccctgc ctagtcagct tttttcctct gttgtttata aacacgaaat cgctaaagtc
     2881 acccggtttg atgagagaat gtcttagccg gtcaagatag tggtgaagat attctgccgc
     2941 attatcgctg taagggacaa ccctttcctt gctccctttt ccgaaaacaa gtatcacctg
     3001 atcttttctg aaaatgctgc cgagcttcag ttccgtaagt tccgacacac gaagtccgga
     3061 agaatagaac atctcaagga tcgttctgtc gcggaatcct gaaggggtcg aagtgtcaat
     3121 gacgctgaaa agaagatcaa cttcatcaag agtaaggaac atcggcagag attttttcat
     3181 ttttaccggt tcgagaatct caaaaagttc aggcgagaca aaatcatctt caacaaggaa
     3241 tttaaaaaaa cccctcatgc ttgaaatgct ccttgcaagc gatttgtctg aaaaattttt
     3301 gtcttttgaa agatggatca catattcact tatattgaca ggatcagccg ttgtaagaga
     3361 tgttttccgc tcttcaagat agtttagaaa atcggtcaga tccctttcgt atgcctcaac
     3421 gctgttaagt gcaagccgtt tttcaagttt gacgaaattg agatattttt tcagaagccg
     3481 tccatcagat ccgatcaaat agatctcccg ttaatctttt ttcaacataa aaaggggctg
     3541 accggtctta acactgaccc cttgagcgat cataggttct ccaaccattt tttcagggat
     3601 cagaagaact attgtcgaac ccatttcgaa tcttccaagt tcctgattct gcttatattt
     3661 tttattcacc agcgtccact ctccgtcaac cggctcttca aaaaaatcca ttttaagaga
     3721 acccacaaaa gaggcaccta ctgcaacata gaagtattgc atcccgtcat gttccatttt
     3781 gacaacaact ctttcgttcc ttatgaaaag tttttcaata gtgttcacag agagttcgtt
     3841 cactggaaaa aggcgccatc cggtgtggat gtatgactta actactgaat cggcaggaac
     3901 atgatatctg tggtaatctt ttggtgaaag atatatcgtc acaagatagt aaccatcggg
     3961 aaaatcgagt tcttttctaa tcagttccga tattggatag gttcttcctt tgacctgcgt
     4021 tgcaatgtcg gcatttattt tttcaagcac agtgatcttt ccgtcactgg gtgaaagaaa
     4081 tgcgttttcg tcattaacga gcggccggac atcactgtca agcttcctca caaaaaaaga
     4141 tgacagcgaa ttataatccc cgacatctcc ttcatagtct ttcatatcta ttccataatg
     4201 gtcacggtaa agctctatca cctttttcac tatccactga ggtgtttcta tctttgcgat
     4261 cgcaccccac gccctgctga ggaatggaag tgaaataata aaaaggaaaa gaagttttaa
     4321 atagctcata taaacccttt aaaatattga caatataaga tctgtcaaac tgtttataat
     4381 gtctctgtta aacctgtaaa aaggtcctga aatccctgat atccacatta taagtgcaat
     4441 aaaagccgca acggctgttg ttataaacag gtttcttttc ataactcttc ttttacgaag
     4501 tttacagcat ttacaaatat ctgcaagccc attccttttt cgccacccgt cccttttctt
     4561 gtccattcag gatgctgagt gccgtttatg aaaacttcag ggtgaggcat cagtcccaga
     4621 acattcccgc ggctgttggt tatgcccgct attgcaagat cgctgccatt cggattttcc
     4681 ggatagttcc ttgtcggttc gccattttta tcaacatact ggaaagcaat aaggtcatcg
     4741 tttctgatct cccttaaaaa atggcggtgt gcaacgaact tcccttcacc gtgtcttatg
     4801 gcgacatcaa aagaatctat ggctcttaag aatggtgact tgttcgttgg atttgccttt
     4861 attctgaccc atctgtcctc atatttcgca gagtcgttgt gggtaagtgt cgcaacctgt
     4921 tttttataca catctctggg atctccggga a
//
LOCUS       DAOOYB010000207         4933 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_19874_length_4933_cov_7.224067, whole genome shotgun sequence.
ACCESSION   DAOOYB010000207 DAOOYB010000000
VERSION     DAOOYB010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4933
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19874_length_4933_cov_7.224067"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3644
                     /gene="rpoB"
                     /locus_tag="PL056_10710"
     CDS             <1..3644
                     /gene="rpoB"
                     /locus_tag="PL056_10710"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF001616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HPM47527.1"
                     /translation="RSPGTFFDFDKAKTTSGNEIFMSRLIPYHGSWLDFEFDSKRLLY
                     VKIDRKKKFPATIFLKAWGYTDQDIISSFYIPEKITINNGIIAKVIDFNRLDILKQQK
                     IPVDVMASDEKEIAKEGKKFSAGTLKKLQDAGITEIALPADYLSGKRLFNPVYDPKSG
                     DMIADVNDDLTATLLDNIIGKGIKEFDIIYFENFNFSPYLSNTLRIDKVATKEDSLLE
                     IYRLMVPSNPVKLEVAEAYFNNLFQNPESYDLSEVGRLRMNHKFNENIPLSMRQLRKE
                     DAMNTVLYILNLSDGRTGYKTDDIDHLGNRRVRLVGELLEDVFKTGLLRMSHSIKEKL
                     AGSVVETLTPYEVISYKQVSAVLKEFFQGGQLSQFMDHTNVLSEMTHKRRLSALGPGG
                     LTREHASFEVRDVHKTHYGRICPVETPEGPNVGLIVSLTTFARIDEFGYIETPYKVVK
                     NCKVTNEIVYLDAIDEEGKTIIPSSVALDSKGKIADEIVNARKSGEVARVRREDVTHM
                     DLVPVQLVSVSAALIPFLEHDDANRALMGSNMQRQAVPLIKTDAPLVGSGMEKYVARD
                     SGYTIFAHRDGVVVKIDANRVVVKVSDEENVDVDIYNLTKFKRSNQNTAINHRPVVSV
                     GDYVRKGDTIADGPAIDRGELALGKNVLIAFMPWNGYNYEDSILINQRLLREDTYTSI
                     HIEEFICEARDTKRGPEEITADIPNAPDEILRKLDESGIIRIGQKIKPGDILVGKVTP
                     KGETVLSPEEKLLRAIFGEKAGDVKDSSLYVHSGVEGVVIDTRIFSRKGVKKDNRMKE
                     QEQIQIDSVKRNRDDEIRIIEKHTYELIRKMLAGKKIDADIKSEKDVVIFKKGTELTE
                     ENTLKIPENVIASVSVKDRDTLKNIKELILGMRRKIETVKKIYENKIRQITRNDDLSP
                     GVIKSVKVFVAIKRKLSVGDKMSGRHGNKGVVSMLLPEEDVPYMEDGRAVDIVLNPLG
                     VPSRMNIGQILEVHLGLGARRIGEQINEFIKENYSLSKLKKELKDILKPNAEIEKLID
                     SSDEDEIRSFIKSMKGGVYTASPVFDGAKESEIREILRKAGEKESGKMILFDGRTGEP
                     FESDVTVGVMYFLKLHHLVDEKVHARSTGPYSLVTQQPLGGKAQFGGQRLGEMEVWAL
                     EAYGAAYCLQEFLTVKSDDVEGRKKLYEAIVRGKTKYDIGIPASFSVLVKEMQSLCLD
                     VNLIEEQNK"
     gene            3655..>4933
                     /locus_tag="PL056_10715"
     CDS             3655..>4933
                     /locus_tag="PL056_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015349106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HPM47528.1"
                     /translation="MKDIFKIFEKPKSSFDFKAIEVSLASPEKILEWSYGEVKKPETL
                     NHRTFKPEKDGLFCAKIFGPVNDYECLCGKYKRMKYKGIVCEKCGVEVIESKVRRDRM
                     GHINLATPVAHIWYVKTTPFYINTVLGMSNSALSSVIYCEGYVVLESQIPGVSRGDYL
                     EEEQYTELIEKYGYDKIRAETGAAPLRKLLEEIDLLAVQKELEEKILTSGDKSQQAKY
                     QKRINVINKFLRSGESPHNMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINR
                     NNRLKKLMDLGAPDVIIKNEKRMLQEAVDALFDNTKVHRPITGSNGRPLKSLSDMLRG
                     KQGRFRQNLLGKRVDYSGRSVIVVGPELRLHQCGLPKIMALELFKPFIYNRLLDRAHV
                     STVKAAKQMVENGEDVVWDILEEVVKEHPVLLNR"
BASE COUNT         1633 a          920 c         1088 g         1292 t
ORIGIN      
        1 accgttcgcc cggaactttt tttgattttg ataaagcaaa aacaacgagt ggaaatgaaa
       61 tttttatgtc aaggcttatc ccctatcatg gatcgtggct tgattttgaa tttgattcca
      121 agcgattgct ttatgtgaag atagaccgca agaagaaatt ccctgccaca attttcctca
      181 aagcatgggg atatactgat caggatatca tttcatcatt ctatatccct gagaagataa
      241 ccatcaacaa cggtattatt gctaaagtta tagatttcaa cagactggac atactaaaac
      301 agcagaagat acctgttgat gttatggcgt cggatgaaaa ggaaattgct aaagagggga
      361 aaaaattctc cgcgggcact ctaaaaaaac ttcaggatgc cggaattact gagattgcgc
      421 tgccggctga ctacctttcg ggcaaaagac ttttcaatcc ggtatacgat cctaaatcag
      481 gcgatatgat cgcagatgta aatgacgatc ttacagcgac tcttcttgat aatattatcg
      541 gaaaaggaat caaggaattt gatataatat attttgagaa tttcaatttc tcaccttatc
      601 tttcaaatac attaagaata gacaaggttg caactaaaga agactctctt cttgaaatat
      661 acagactcat ggttccttca aatcctgtta aacttgaagt tgctgaagca tatttcaaca
      721 atcttttcca gaatcctgaa agttatgatc tcagcgaagt cggtcgtttg agaatgaatc
      781 acaaattcaa tgaaaacatt cctctttcaa tgagacagct ccggaaagaa gatgctatga
      841 atactgttct gtatattctt aatctcagtg atggcagaac aggatacaaa acagatgaca
      901 ttgatcacct tggcaaccgt agagtccgtc ttgtaggaga acttcttgaa gatgttttta
      961 aaacaggttt attaagaatg tcccacagca tcaaggaaaa acttgcaggc agtgttgtcg
     1021 aaacacttac accgtatgaa gtgattagct ataaacaggt cagtgccgtt cttaaagagt
     1081 tcttccaagg tggacagttg agccagttca tggaccatac aaatgtcctt tcagagatga
     1141 ctcacaaaag gcgtctttct gcacttggtc caggcggact tacaagagaa catgcaagct
     1201 ttgaagtaag agacgttcat aagacccatt acggccgaat atgtcctgtt gaaacacctg
     1261 agggtccgaa tgtcggtctt atcgtatctc ttacaacatt cgccagaatt gatgaattcg
     1321 ggtatattga aacaccttat aaagttgtca agaactgtaa agttactaat gagatcgtat
     1381 atctcgatgc tatcgatgaa gaaggaaaaa caatcattcc gtcgtctgta gctcttgact
     1441 caaaaggaaa gatcgctgac gagatagtaa acgcaagaaa atcaggcgaa gttgcaagag
     1501 tcagaaggga agatgtaaca catatggacc ttgtccctgt tcaacttgta tccgtttccg
     1561 ctgcactgat ccctttcctt gagcatgatg atgcaaacag ggctttgatg ggatcaaaca
     1621 tgcagagaca ggctgttcct ctgatcaaaa cggatgcgcc tcttgtagga agcggaatgg
     1681 aaaagtatgt tgcaagagat tcaggatata caatttttgc acatagagac ggtgtagttg
     1741 taaaaataga tgcgaacagg gttgttgtta aagtttcaga tgaagaaaat gttgacgtag
     1801 acatctacaa tcttacaaaa tttaaaagat caaaccagaa tacagccatc aatcacagac
     1861 ctgttgtgtc tgtaggcgac tatgtaagga aaggtgacac aatagctgac ggtccggcta
     1921 tcgacagagg agagcttgca ctcggtaaaa atgttctcat cgcattcatg ccgtggaacg
     1981 gatataacta cgaggactct atccttataa atcagcgcct tttaagagaa gacacctata
     2041 cttcaataca cattgaagaa tttatatgtg aagctcgtga cacaaaaaga ggtcctgaag
     2101 agataactgc agatatccct aatgctcctg atgaaatatt aagaaaactc gatgaaagtg
     2161 gtattatcag gatagggcag aagatcaaac ccggtgatat tcttgtaggt aaggtcactc
     2221 ctaaaggtga aactgttctt tctcctgaag aaaagctttt aagagcgata ttcggtgaaa
     2281 aagcaggcga cgtaaaagac agttcactct atgttcattc aggtgttgaa ggtgttgtaa
     2341 ttgacacaag gatattctcc agaaaaggtg tcaaaaaaga taacaggatg aaggaacagg
     2401 aacagatcca gatagattcc gtgaaaagga accgtgacga tgaaataaga attattgaga
     2461 aacacactta tgaacttatc cgtaaaatgc ttgccggcaa aaaaattgac gccgatataa
     2521 agagcgaaaa agatgttgtc attttcaaga aaggaactga acttactgaa gaaaacactc
     2581 tgaaaattcc cgaaaatgta atcgcatccg taagtgttaa ggacagggac actcttaaaa
     2641 acattaaaga gctcatcctc ggaatgagaa gaaagatcga aactgttaaa aagatctatg
     2701 aaaataagat ccgccagata acaaggaacg atgatctttc gccgggtgta ataaaatcag
     2761 tgaaagtgtt cgttgctatc aaaagaaaac tttctgtcgg tgataaaatg tcaggccgtc
     2821 acggcaacaa gggtgttgtg tcaatgctcc tgccggaaga ggatgtccct tacatggaag
     2881 acggaagagc cgttgacata gttctcaatc cgctcggcgt tccgagccgc atgaacatcg
     2941 gtcagatact cgaagtccat cttggtctcg gagctagaag gatcggtgag cagatcaatg
     3001 aatttataaa agaaaattac agtctttcca agcttaaaaa agagctgaag gatattctta
     3061 aaccgaatgc cgaaattgaa aaactcatag attcatcaga tgaagatgaa atccgcagtt
     3121 ttataaaatc gatgaaaggc ggtgtttata cagccagccc tgtttttgac ggagcaaaag
     3181 aaagcgagat cagagaaata cttagaaagg ccggcgaaaa ggaaagcggt aaaatgatcc
     3241 tttttgatgg tcgtaccggt gaaccttttg agtctgatgt aaccgtcggt gtaatgtatt
     3301 tccttaaact tcaccatctt gttgatgaaa aagttcacgc aagaagcact ggtccttact
     3361 cacttgtcac acagcagccg ctgggcggaa aagctcagtt cggtggacag cgtcttggag
     3421 aaatggaagt ctgggctctt gaagcatacg gagcggcata ttgtctgcag gaatttctca
     3481 cagtaaaatc tgacgatgtg gaaggaagaa aaaaacttta cgaggcgatc gtaaggggaa
     3541 aaacaaaata cgacatcgga atacctgctt cgttttccgt tcttgttaaa gagatgcaga
     3601 gtctgtgcct tgatgtaaat ttgattgaag aacagaataa ataaggagga taccttgaaa
     3661 gatattttca aaatctttga gaaaccgaaa agcagttttg attttaaagc tattgaggtg
     3721 tcactcgctt ctcctgaaaa gatccttgag tggtcttacg gcgaagttaa aaaaccggaa
     3781 actctgaacc acagaacatt caagccggaa aaagacggtc ttttctgtgc aaagatattc
     3841 ggacccgtaa acgactacga gtgtctgtgc ggaaaatata aaaggatgaa atataaaggc
     3901 atagtatgtg aaaaatgcgg tgtagaagtt attgaatcca aggtcagaag ggaccgtatg
     3961 ggtcatatta acctcgccac accggttgca cacatctggt atgtaaaaac aacccctttc
     4021 tacattaaca ccgttctggg tatgtccaac tcggctctaa gcagtgtaat ttactgtgaa
     4081 gggtatgtag ttcttgaatc ccagatccca ggggtttcaa gaggcgatta tcttgaagaa
     4141 gagcagtaca ccgaactcat tgaaaaatac ggttatgata agatccgtgc agaaacaggt
     4201 gccgctcctt taagaaaact tcttgaagaa atagatcttc ttgcagtaca gaaagaactt
     4261 gaggaaaaga tcctcacatc aggtgacaaa agtcagcagg caaaatatca gaaaaggatc
     4321 aatgtcatta acaagttcct caggtctgga gaaagtcccc acaacatgat attaacggtg
     4381 cttcccgtcc ttcctccgga tctcagaccg cttgttcctc ttgatggcgg aaggtttgcg
     4441 accagcgatc ttaacgacct ttacagaagg gttataaaca gaaacaaccg acttaaaaaa
     4501 cttatggatc tcggtgctcc ggatgtcatt atcaaaaacg aaaagagaat gcttcaggaa
     4561 gctgttgacg ctctttttga caatacaaag gttcatagac caataacagg atctaacggc
     4621 agaccgctta aatctcttag tgacatgctt agaggtaaac agggtcgttt ccgtcagaat
     4681 ctgcttggaa aacgtgttga ctactctggt cgttctgtaa ttgttgttgg tcctgaattg
     4741 agacttcacc agtgcggatt gccaaagatt atggctcttg agcttttcaa gccgtttatc
     4801 tataacagac ttcttgacag agcgcatgtt agtactgtta aagctgctaa acagatggtt
     4861 gaaaatggtg aagacgttgt ttgggatatt cttgaagaag ttgtaaaaga acatccagtt
     4921 cttcttaacc gtg
//
LOCUS       DAOOYB010000208         4932 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_19881_length_4932_cov_9.136764, whole genome shotgun sequence.
ACCESSION   DAOOYB010000208 DAOOYB010000000
VERSION     DAOOYB010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4932
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19881_length_4932_cov_9.136764"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="PL056_10720"
     CDS             <1..455
                     /locus_tag="PL056_10720"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HPM47529.1"
                     /translation="FYFGPALSREGHFFVDREDNSSMRKAAKDLLKFSKDFCRIIIFP
                     EGKASPDAKRLPFKPGAFAMAKKLDKTVQPCVIDYLPERSVHKWDTTKKGLPQLINYL
                     GKKRIDVSIEFFPAVKIEGETKEFAEKWHDIIENMLKHYDSERDGNRI"
     gene            439..1104
                     /locus_tag="PL056_10725"
     CDS             439..1104
                     /locus_tag="PL056_10725"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPM47530.1"
                     /translation="MAIEFNGKTIKAVIFDLDGTFYRIGFFTKMAFLFHNIRHLTLFS
                     AHRKVMKELRKEDHGSGEKYFDTLFGRISGLTGTEKTNVKKWYLTDFRKDFISALAKN
                     CIKNKSIERVACTLRNLNFKTAVLSDFGFIEERLDALNIDRKLFNVLVSGEEEGALKP
                     QTRPLMKIFGELGVLPENAVLIGDRNDTDGAVAENAGMIFINVRNIGAFEKNLKINIS
                     GVV"
     gene            1104..>4932
                     /locus_tag="PL056_10730"
     CDS             1104..>4932
                     /locus_tag="PL056_10730"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HPM47531.1"
                     /translation="MKRYDIAIIGMGGVFPGSKNVEEYWKNVLSGRTFIKDMPKKYWR
                     WENYYSEDTSVIDKSYTKKGSFIDDFEFPFSKYKFPPNTMKGVDPAQLVALESAREAL
                     EDAGIKPHSPALDEAVTILGVSGVDDYAHACAFLRRHNYFDKLRKQLRENGVSENEIN
                     ELSKQFDEEILKRHSVKMSLLAVGVIPSALSNRVAQVFGAKGYNMTVDAACASSFGAL
                     ETACHALMAGDARVAITGGVDLGVNPAIYIGFCRVDGISFSGISNPFDYKADGLVIGE
                     GGGVIILKKLEDALADGDRIHAVIRGMGSSSDGAGQAIYSPSVEGRARAFRMALKRAD
                     LASHDVQFVEAHATSTVVGDANEYEAIAAAYSDGRKVDNPLYLGSVKYQIGHLKAAAG
                     VAGLIKTIKGMENGIVPHMPLFEKLTPNVKNPVEAFVIPTKPVKWEPNSTGKKIAAVT
                     SSGFGGCNYHVIIEQGENYVPVNKRKKINRDIAVVGVTCRVAGADDVNTFWDNVTAGK
                     NVFSNVDPDELNWKFHFNTGPKDENIYSTKISKLKPYSLDYLKYKILPKSVSQISPTQ
                     FLGLDLAGRLLEGNGYNITEPKNIGVSIGAIHDDYYPDIFDPMITDDYAGAIRNTPAA
                     SGIDPKILENSINETKKEVTATTPPITEHTLPGWMGNIVAGRMANKLNLQGPNMVVDS
                     ACSSGLSAMVPAIYQLAFGDVDMMISGGLNRQISDVFTAGVCAINAVAKDEARPYDEE
                     GAGYVIGDGGVLYLLKRLEDAKRDGNEIIAVIHSINGSSEAETKSMIAPTEKAVRRAI
                     TKSLEKTYIDKSRIGVVDTHGSANRASDVIEAISVAEEIGNEKTDIPVSITAIKSHIG
                     HLYGGSGASSMLSVIQTLRTRKVPGIRNLENIRNEIMEHIDKATPVKKTTPLDDKYDA
                     GGVLSLGLGGTNYFAVVSLGANQSGKGEKMIPKTETPKMQMNTERPENGTGIFTGSSP
                     DMKSLSGVIEKCISNGVVSSSDFNENDSVRFALTYKNENDLISKLRSTAKFISSGHDV
                     APLENQGIFISSDDLKDEKLAFCFPGQGTHYISMGRFLYESNATFRNIIDEVNGLAKN
                     DMNFDLVGHIYGDPENKDIEKNLGTLTGAQTSLFAIEVALAKMLEEKGIIPDVLIGHS
                     FGEISALTFAGVWDIPTAFKVVKARIEAAEFGKQSAPFPLGMMSINCSEEKRDRLLSF
                     AGENLILTNINAPGRYIFAGELELVKRTVELAESFGLEARVLPIGSAFHSKFMEPAKE
                     KYYSLLSKLSCSNP"
BASE COUNT         1612 a          979 c         1121 g         1220 t
ORIGIN      
        1 cgttctattt cgggccggca ctttcaaggg aaggacactt ctttgttgac agggaggaca
       61 attcttctat gaggaaggcg gcaaaagacc ttcttaaatt ctcaaaagat ttctgcagaa
      121 taatcatctt ccccgaaggc aaagcgagtc ccgacgcgaa aagacttccg tttaaaccgg
      181 gcgcttttgc aatggcaaaa aaactggata aaaccgttca gccctgcgtt atcgattatc
      241 ttcccgaaag gtctgttcat aagtgggata caacaaaaaa agggctccct caactgatca
      301 attatcttgg gaaaaaaaga atcgatgtca gcatagaatt cttccctgcg gtcaagatcg
      361 aaggagaaac gaaagaattt gccgaaaaat ggcatgatat aattgaaaac atgcttaaac
      421 attatgacag tgaaagagat ggcaatagaa tttaacggaa aaaccattaa ggcggtcata
      481 tttgatctgg acggcacttt ttatcgaata gggtttttta caaaaatggc ttttcttttt
      541 cacaacatca gacacctgac acttttttcc gctcacagaa aagtcatgaa agaactccga
      601 aaagaagatc acggatcagg tgagaaatat tttgatacac ttttcggccg catatcaggt
      661 ttgacaggta ccgaaaagac aaatgttaaa aaatggtatc ttaccgactt cagaaaagat
      721 tttatcagtg ctctggcaaa aaactgcatc aagaacaaat caattgaaag ggttgcctgt
      781 acactgagaa atctgaattt taaaacagca gtcctttccg atttcggatt tattgaagaa
      841 cggcttgatg ccctgaacat agacagaaaa ctttttaatg ttcttgtttc gggagaagag
      901 gaaggtgcac tcaagcccca gacgagaccg cttatgaaga tcttcggcga actcggtgtt
      961 ctgcctgaaa acgctgtcct tataggtgac aggaatgata cggacggtgc tgtggcggaa
     1021 aatgcaggaa tgatttttat taatgtcagg aacatcggtg cttttgagaa aaatttaaaa
     1081 attaacatat cgggagttgt ttaatgaaaa ggtatgacat cgcgattatc ggaatgggtg
     1141 gcgttttccc cggttctaaa aatgttgaag agtactggaa aaatgttttg agcggaagga
     1201 cattcataaa agatatgccg aaaaaatact ggcgctggga aaattattac agcgaagaca
     1261 cttcggtaat tgacaaatca tatacaaaaa agggatcgtt cattgatgat tttgaatttc
     1321 ctttcagtaa gtacaaattc cctccgaaca caatgaaagg tgttgatcct gcacagcttg
     1381 tggcacttga atcagcaaga gaagctcttg aagacgcagg gattaagccg cacagcccgg
     1441 ctcttgatga agccgttaca attctcggtg ttagcggggt agatgattac gcccatgcct
     1501 gtgctttttt aagaaggcat aactattttg ataaattgag aaaacagctc agagaaaacg
     1561 gagtttcaga aaatgagata aacgaacttt caaagcagtt tgacgaagag attttaaaaa
     1621 gacattctgt aaaaatgtcc ctccttgcag taggtgtcat tccttcagct ctttccaatc
     1681 gtgtagctca ggttttcgga gcaaagggct ataatatgac agttgacgcc gcatgcgcat
     1741 cttctttcgg agcacttgaa actgcatgtc atgcattaat ggcgggagac gccagggtcg
     1801 caataacagg cggagtggac cttggagtaa atccagcaat ttacatcggc ttctgccgtg
     1861 ttgacggcat aagcttttca ggaatatcaa atccctttga ctacaaagcc gacggtcttg
     1921 taatcggtga aggaggcggt gtaataattc ttaaaaaact tgaagatgcc cttgctgatg
     1981 gagacagaat acatgcagtt atccgtggaa tgggcagttc aagtgacggt gcgggtcagg
     2041 cgatttacag tccatctgtc gaagggcgtg cgagagcgtt cagaatggct cttaagaggg
     2101 cagaccttgc ttcgcacgat gttcagttcg ttgaggcaca cgcaacttca actgttgtag
     2161 gcgatgcaaa tgaatatgag gcaatcgccg cggcatattc cgatggcaga aaggttgaca
     2221 acccgcttta tcttggaagt gtaaaatatc agattggaca cttgaaggcc gctgcagggg
     2281 ttgccggact tataaaaaca ataaaaggca tggaaaacgg gattgttccg cacatgcctc
     2341 tttttgaaaa actgacacct aatgtcaaaa acccggttga agcttttgtc attcccacaa
     2401 aaccggttaa atgggaaccg aatagtacag gcaaaaagat tgcggcagta acttcaagcg
     2461 gtttcggcgg atgtaactat catgttataa ttgaacaagg cgaaaattat gttcctgtaa
     2521 acaagaggaa aaaaataaac agagacatcg ctgttgtcgg agtaacctgc agagttgcag
     2581 gggcagacga tgtaaacact ttctgggata atgttacagc agggaaaaat gttttttcaa
     2641 atgttgaccc cgatgaattg aactggaaat tccattttaa tacagggccc aaggatgaaa
     2701 acatctattc gaccaaaata tcaaaactta agccatacag tcttgactat ctcaaataca
     2761 aaatacttcc gaaatcggtc agtcagatct ctcccacgca gttcctcgga cttgatcttg
     2821 caggacggct tcttgagggt aacggataca atataactga acctaagaac atcggagtaa
     2881 gcatcggagc gatacatgat gactattatc ccgacatttt cgacccgatg ataactgatg
     2941 attatgccgg agcgatccga aatacaccgg cagcatcagg gatagatccg aaaatacttg
     3001 aaaattcaat aaacgaaacc aagaaagagg ttacagcaac aacaccaccg ataacagaac
     3061 acacacttcc cggctggatg ggaaacattg ttgcaggcag aatggcaaac aagttgaacc
     3121 ttcagggtcc caacatggtc gttgattccg cctgttcatc gggattaagc gcaatggttc
     3181 cggcaatata tcagcttgct ttcggtgatg tggatatgat gataagcgga ggactgaacc
     3241 gtcagatcag cgatgttttt actgccggag tatgtgctat caacgctgtt gctaaagatg
     3301 aagcaagacc ttacgatgaa gaaggtgccg gatatgtcat aggtgacgga ggagtccttt
     3361 atcttcttaa acggcttgaa gatgcaaaaa gggacggaaa tgaaataatt gccgtcatcc
     3421 attcaataaa cgggagttcg gaagcagaaa caaagtcaat gatcgctcct accgaaaaag
     3481 ctgttagaag ggccatcacc aaatcactgg agaaaacata cattgacaag agcagaatag
     3541 gcgttgtcga tactcatggt tccgctaaca gagcaagtga tgtgattgaa gcgatcagcg
     3601 tagcagaaga gatcggaaac gaaaaaacag acattcctgt ttcaatcacc gcaataaaaa
     3661 gccatatcgg acacctttac ggaggatcag gtgcatcatc aatgctgagc gttattcaga
     3721 cactgagaac cagaaaagtt ccaggaataa gaaaccttga aaacatcaga aatgagatca
     3781 tggagcacat tgacaaagcg acacctgtaa aaaagacaac tcctcttgat gacaaatatg
     3841 atgccggagg agtcctttca ctgggtctgg gaggaacaaa ctattttgca gttgtgtcac
     3901 ttggcgctaa tcaaagcgga aaaggagaaa aaatgattcc taagaccgaa acaccaaaaa
     3961 tgcaaatgaa cactgaaaga ccggaaaacg gaaccggcat atttacagga agctcacctg
     4021 acatgaagtc attaagcggc gtgatcgaaa aatgtatctc aaacggagtt gtaagctcat
     4081 cggatttcaa tgaaaacgac tctgtccgct ttgcacttac ttataagaat gaaaatgatc
     4141 tcatttcaaa actccggtca actgcaaagt tcatctcttc aggacacgat gttgcaccac
     4201 ttgaaaatca ggggatattt atctcatctg acgatcttaa agacgaaaaa ctcgcattct
     4261 gtttccccgg acagggaaca cactacattt caatgggaag attcctctat gagtcaaatg
     4321 caacattcag gaacatcatt gatgaagtga acggtcttgc gaaaaatgat atgaactttg
     4381 atcttgtcgg acacatttac ggagatcctg aaaacaaaga tattgaaaaa aatcttggaa
     4441 cactgacagg agctcagacc tctctgtttg caattgaagt agcccttgca aaaatgcttg
     4501 aggagaaagg aataatcccc gatgttctca tcggacacag ctttggcgag atatcagcac
     4561 ttacatttgc aggtgtatgg gatatcccga ctgcatttaa agtggtcaaa gcgagaatag
     4621 aagctgcaga attcgggaaa cagtcagctc cgttcccgct tggcatgatg agtataaact
     4681 gttccgaaga aaaaagagac cggctcctct cttttgcagg cgaaaacctt atacttacaa
     4741 atatcaatgc tcccggcaga tatatttttg caggagaatt ggaacttgta aaaagaaccg
     4801 tcgaacttgc tgaatctttc ggacttgaag cacgggttct gccgatagga agtgccttcc
     4861 attcaaaatt catggaaccg gcaaaagaga aatattattc actcctttca aaactgtctt
     4921 gttcaaatcc ga
//
LOCUS       DAOOYB010000209         4919 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_19962_length_4919_cov_8.998766, whole genome shotgun sequence.
ACCESSION   DAOOYB010000209 DAOOYB010000000
VERSION     DAOOYB010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4919
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19962_length_4919_cov_8.998766"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            73..1329
                     /locus_tag="PL056_10735"
     CDS             73..1329
                     /locus_tag="PL056_10735"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPM47532.1"
                     /translation="MSSLTIEEYLDIVMKGNISIKSKINSSEKSEWSRLQSYSGYLPS
                     LDLGGSFYYSRYNEEQLEANPAIATLKNPLYTRKITMSLPVFVGGNRILGNMIAQKNK
                     EISTVEIENEKLAVEASAVAAYFQTFITQENIKLSEKAVKAATENLRTAKLLLESGRT
                     TELAYLNLELVLRKREQELENYKMEMLKNIADMSRIAAVEIEFDELERSDFGVIEDHF
                     SGKDHKTLFEDKKSLLLNNSPLLIKMEKLKKISDYSTVMKMAPFLPVLNFSFVHDFGS
                     AEDHPFNKTYLYDDDVVSLNFSWNLFKGFNDTIEWRKSLKDKVASQLAYLETKNSQMY
                     SLKAVLASIFSLLEQKKVALSSVEISQKALEKSKIEFENGKSLYLDLLNAENSYLEAL
                     KNLVLVENSIFNSFYQLRILTGGKEE"
     gene            1326..2474
                     /locus_tag="PL056_10740"
     CDS             1326..2474
                     /locus_tag="PL056_10740"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPM47533.1"
                     /translation="MKHLLLFISAFVFLLATSGCSKDTAGTALEKQDGAAVPENTAGV
                     QKRAPRKVRIQKIVPGDVTGKIFFNAVIEAERDVTVTSKTTGEIVEMNYDIGSFVKKG
                     ATVAVIEYDLQRTSLDQAELNLKQAELTFALKEKVFQRDRKLFENKAITAEQFDASEN
                     GYQSAELSLNQAKTSLQNATVNYNNCFIKAPFDGIVVERPVQQGQYVSIGTAIARIVD
                     TANLQVVVGMSPDDLLIYKRYKNKDVGIILPNDQVVPGTVKGVADAPDRKTSLYSMKI
                     LFKSVKDENDKYIVFPGMQLKVAIDSKVHKNTFLTSRNNMRLLGNRYIVYIIREGKAV
                     EKEVEIVSDIGKERVVRFSDGYSEPSELIISGLEALSDGREVEVLEEK"
     gene            2471..>3002
                     /locus_tag="PL056_10745"
     CDS             2471..>3002
                     /locus_tag="PL056_10745"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPM47534.1"
                     /translation="MSFVQFFLKKPIVAHFIFVAVMTFGIIATQTIPVEETPEINLGF
                     VIVITLYPGAAPQEIERLITIPIEDAVANVEDIDYINSGSRNGKSSVFINFLETVKDI
                     DKRVIDIQTEINKIPDLPSKNEMAGPYVFKIATGDTSPVINVMLSSDSISDNDFRDVA
                     ENLQRELTSKISGIKNV"
     assembly_gap    3003..3102
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3103..>4919
                     /locus_tag="PL056_10750"
     CDS             <3103..>4919
                     /locus_tag="PL056_10750"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPM47535.1"
                     /translation="AENLQRELTSKISGIKNVEVAGVSEEEIQIVVSNEKLMGFNLAI
                     DDVFAALTRSNFRTPGGTLDISGKRYLVKATGTFDDVEKIEQVLVKTGANGQKLYLKD
                     VAEVQKKFIRGNIESYLNGRKAVSLYIMRKPESNIVAISERVREVVPEFAKNYADLEY
                     SFKNDQGEAIDETMSVLRDNAMLGMVLVSLLLFIFMGWRASFLAVIGIPFSFLATFFM
                     MKFLGYTINSLSLFAMILVSGMLVDDAIVVIENVYRYLEDGFAPFDAVMKGTQEIMWP
                     VISAIATTICAFLPLLMITGMIGKFITQFPIIVTLTLTSSLLEALLVLPVHLHEMKNV
                     YNSQKKPEKKWYKKFLNGYGQILRFILRFRYIAVVVLFIVFAGSIFIASSLRIVLFGD
                     ENAKTVTAKMELAENTPIEKTREISRLIENYVIKELMPEDVESVVTIVGRVIEDRRWI
                     EKEEVAEFRLDLKDYDDKMMNKVKEALRKRVTTIPEIVEFEFLKSTGGPPTGRAVDIS
                     VAGKDLDKLEKVGDEIIAFAKSIPGVVDLGGSVMSKINEIVVEPDFSRLEQTGVSLAS
                     LAETIRSVTAGKYAGRYLNTDGKELKIWIRFDSSGTYTL"
BASE COUNT         1543 a          842 c         1099 g         1335 t
ORIGIN      
        1 tcttaatcaa cttctctgtc agattttatc cgtcaaacct tttcggagtg ggctcttata
       61 ataaatttta atgtgtcttc tctcacgata gaagagtacc ttgatattgt tatgaaaggg
      121 aatatatcaa taaaatcaaa gattaacagt tctgaaaaga gcgaatggtc aaggttgcag
      181 agttattcgg gttatcttcc atccctcgat cttggcggga gcttctatta cagcagatat
      241 aatgaagagc agcttgaggc gaatccggcg atagcaacct tgaaaaatcc gctttatacc
      301 aggaagataa cgatgtctct tcctgttttt gtaggcggga acaggatctt aggcaacatg
      361 atcgcacaaa agaataaaga aatttcaact gtcgaaattg agaatgagaa actggcagtt
      421 gaggcatcgg cagttgcggc ctattttcag acattcataa cgcaggaaaa catcaagctt
      481 tctgaaaagg cagttaaagc ggcaactgaa aatttgagaa ctgctaaact gcttcttgaa
      541 tcgggaagaa cgaccgaact ggcatatctt aatctcgaac ttgttctcag aaaaagagag
      601 caggaactgg aaaactataa aatggaaatg cttaaaaata ttgcggacat gtcaaggatc
      661 gccgcggttg aaatagagtt tgatgagctt gaaagatcag atttcggtgt catagaagat
      721 catttttcag ggaaagatca caaaactctg tttgaagata aaaaatcact ccttctgaac
      781 aattcaccgt tacttatcaa aatggaaaaa ctgaaaaaaa tatctgatta cagcacggtc
      841 atgaaaatgg cccctttcct gccggttctg aacttcagtt ttgtccacga tttcggatcg
      901 gcggaagatc atccattcaa caagacatat ctctatgacg atgatgtggt atcgctcaat
      961 ttttcatgga atctttttaa aggtttcaac gacacgatcg aatggagaaa gtcgcttaaa
     1021 gacaaggtgg catcgcagct ggcatatctt gaaacgaaaa attcgcagat gtattcgttg
     1081 aaagcagtac ttgcttcgat attctcactt cttgagcaga agaaagttgc tttgtcttca
     1141 gttgaaatat ctcaaaaagc acttgaaaag agcaaaatag agttcgagaa cgggaaatca
     1201 ctctatctgg accttctcaa tgccgagaac agctatttgg aggcattgaa aaatcttgtt
     1261 cttgttgaaa attcaatttt taacagtttt tatcagctga gaattctaac ggggggaaaa
     1321 gaggaatgaa acatctctta ctttttatat cggcatttgt ttttcttctt gcgacttcag
     1381 ggtgttcaaa agatactgcc ggcacagctc ttgaaaagca ggatggtgcc gctgttcctg
     1441 aaaatacagc aggtgttcag aaaagagctc cgagaaaagt ccgtatccag aagatagttc
     1501 cgggagatgt tacaggaaaa atatttttca atgcggtgat cgaagcggaa cgagatgtaa
     1561 ccgtgacatc aaaaacaaca ggcgagatag ttgaaatgaa ttatgacatc ggctcttttg
     1621 tgaaaaaagg ggcgactgta gcagtaatag aatatgatct ccagcgaact tcacttgatc
     1681 aggcggaact taacctgaaa caggccgaac tcacttttgc tcttaaggaa aaggttttcc
     1741 agcgcgatag aaagctgttt gagaacaaag ccataactgc tgaacagttc gatgcttcag
     1801 aaaacggtta tcagagtgct gaactttcac tcaatcaggc aaaaacatcg ttgcagaatg
     1861 ccacagtgaa ttacaacaac tgctttatca aagctccttt tgacggaata gtcgttgaaa
     1921 gaccggttca gcagggtcag tatgtaagta ttggcacagc tattgcgagg atcgtcgaca
     1981 ctgcaaatct tcaggtcgtt gtaggaatga gccccgatga tcttcttatc tacaaaaggt
     2041 ataaaaataa agatgtaggg ataatacttc ccaatgatca ggttgtgccg ggaacagtaa
     2101 agggggttgc cgatgctcct gacagaaaga catcgctcta ttcaatgaag atactgttta
     2161 aaagcgtgaa ggacgaaaat gacaaatata tcgtgtttcc gggaatgcag ttgaaagtgg
     2221 ccattgattc aaaggttcat aaaaatacat ttctaacaag ccgcaacaac atgcgtcttc
     2281 tcggaaacag atatatcgtc tatatcatca gggaagggaa agccgtcgaa aaggaagtgg
     2341 agatagtttc agatatcgga aaagagagag tggtcagatt ctcagacgga tattcagaac
     2401 cttctgaatt gataatttca gggctggaag ctctttctga cggaagggaa gtggaagttc
     2461 tggaggaaaa atgagttttg ttcagttttt tctcaaaaaa cctatagttg cgcattttat
     2521 atttgtagct gtaatgactt tcggaattat tgcaactcag acaataccgg tagaggaaac
     2581 accggagatc aatctcggtt ttgttattgt gatcactctt tatccgggtg ccgcacctca
     2641 ggagatagag cggcttataa ccattcctat cgaagacgct gtagccaatg ttgaagatat
     2701 tgattatata aacagcggat cacggaacgg aaaatcatcg gttttcataa actttcttga
     2761 aacggttaaa gacattgata aaagggttat agatattcag acggagataa ataaaatacc
     2821 tgatcttccg tcaaagaatg aaatggcagg gccttatgtt ttcaagatcg ctacaggtga
     2881 tacgagccct gtgataaatg tcatgctcag ctccgattcg atctcggaca atgatttccg
     2941 cgatgttgct gaaaatcttc aaagagagct tacatcaaag atttcaggaa taaagaatgt
     3001 cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngctgaaaa tcttcaaaga
     3121 gagcttacat caaagatttc aggaataaag aatgtcgaag tcgccggtgt gtctgaagaa
     3181 gagatacaga tagttgtcag caatgaaaaa cttatgggat tcaatctggc gatagacgat
     3241 gtttttgccg cacttacccg ttccaatttc aggacaccgg gagggacgct tgatatttcg
     3301 ggcaaaagat atctcgtaaa agcgacagga acatttgatg atgtggaaaa gattgaacag
     3361 gttctcgtga aaacaggggc aaatggacag aaactttatc tcaaagatgt tgctgaagtt
     3421 cagaaaaagt ttataagagg taatatagaa tcttatctta acggcagaaa agctgtttct
     3481 ctttacatca tgagaaaacc tgaatcgaac attgtggcta tttctgaaag agtaagagaa
     3541 gtggttcctg aatttgcaaa gaactatgca gatcttgagt acagttttaa gaacgatcag
     3601 ggcgaagcga tagacgaaac gatgtcggtg ctcagagata atgcaatgct tggaatggtt
     3661 cttgtttcgt tgttactttt catttttatg ggttggcgtg caagttttct tgcagtaata
     3721 ggaatccctt tctcgttcct tgccactttc ttcatgatga aatttttagg ttatacgatt
     3781 aattcgctat ctctttttgc aatgatactt gtgagcggaa tgcttgtgga tgatgcaatt
     3841 gttgttatcg aaaatgtcta tagatatctt gaggatggtt ttgctccgtt tgatgctgta
     3901 atgaaaggga ctcaggaaat aatgtggcct gtgatatcgg caattgcgac tactatctgt
     3961 gcttttttac cgcttcttat gataaccgga atgataggaa aatttattac acagttccct
     4021 ataattgtca ctctcactct tacttcttca cttcttgaag ctcttttggt ccttccggtt
     4081 catctacatg aaatgaaaaa tgtatacaac agtcagaaaa agcccgaaaa gaaatggtac
     4141 aagaaatttc tgaacggtta cggacagatt ttaagattca tcctgagatt caggtatata
     4201 gccgtggtag tgctgtttat tgtttttgcg ggttcgatat ttattgcaag cagtctgagg
     4261 atcgtccttt tcggagatga aaatgcaaag acagtcacag cgaaaatgga gcttgctgaa
     4321 aacaccccga ttgagaaaac cagagaaata tcccggctta ttgaaaatta tgttataaaa
     4381 gagcttatgc ctgaagatgt tgaatcagtt gttacgatag tcggaagggt aatagaggac
     4441 agaaggtgga tagaaaaaga ggaagtggct gaattccggc tggatctgaa agattatgat
     4501 gacaaaatga tgaacaaggt caaggaagct ctccggaaaa gagtcaccac aatacctgaa
     4561 atagtggaat tcgaattctt aaaaagtaca ggcggtcctc ctaccggaag agccgttgat
     4621 ataagtgtgg caggaaaaga ccttgacaag cttgaaaaag ttggtgatga gatcattgcg
     4681 tttgcaaaat cgatccccgg agttgtcgat ctcggtggct ctgtgatgag caagatcaac
     4741 gaaatagttg ttgaacccga tttttcacgg cttgaacaga caggcgtgtc acttgcttcc
     4801 cttgctgaaa cgataaggtc tgtgactgcc ggaaaatatg ccggaagata tctgaacaca
     4861 gatggaaagg aactgaaaat atggattaga tttgattcaa gcggaacata tacgcttga
//
LOCUS       DAOOYB010000210         4911 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_19998_length_4911_cov_11.206137, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000210 DAOOYB010000000
VERSION     DAOOYB010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4911
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19998_length_4911_cov_11.206137"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1243)
                     /locus_tag="PL056_10755"
     CDS             complement(<1..1243)
                     /locus_tag="PL056_10755"
                     /inference="COORDINATES: protein motif:HMM:NF014466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HPM47536.1"
                     /translation="MKKLSFIILIFSIIFFASCDSGKKTNVDDNTGVTDTNNITDDEG
                     SSDSLPVTDDEVTVPDKDTVIEKDEESKKDEEQVNDTEEPVDTEVTDTEETEDSDIID
                     LCPENPEKTDPGICGCDVLDVDTDGDGIMDCKDNCTEIKNADQLDTDNDRKGDVCDNC
                     PNHANIKQTDTDKDGVGDACQNDTLLLDTDHDGFRDLVDNCPTIANSDQLDTDSDGVG
                     DVCDNCIYTANNDQTDTDFNGTGDACEPLDSDGDGVLDFNDNCKYIHNSGQEDMDKDS
                     IGDLCDNCPEVANSNQLDYDTNGVGNACEPNDPDGDGIPDESDNCPGTHNPGQVDSDG
                     DGVGDACDNCPAVANADQLDDDSDGTGNVCENVDTDGDTVLDSSDNCPSVPNSDQKDS
                     DGDLLGDACDNCIYDNNEDQAD"
     gene            complement(1324..2028)
                     /gene="aat"
                     /locus_tag="PL056_10760"
     CDS             complement(1324..2028)
                     /gene="aat"
                     /locus_tag="PL056_10760"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007059122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HPM47537.1"
                     /translation="MIYALSPDILDFPPVEHSEPDGLLAIGGDLSPERLLLAYRSGIF
                     PWYSENSEFNYLGNKILWWSPDPRFVIFPEKVHVSKSMGKLLKKGVFEITFNKAFPQV
                     ISRCRHEVRVRQEGTWITDEMEKSYNELHRLGYAISVEAWVAGTGNLAGGLYGVMLGK
                     AFFGESMFSIYENASKAAFITFMQVLQKAKCPIVDCQVETDHLKSLGAEFISRKEFVH
                     TISKAAKKNTIDFNKL"
     gene            complement(2025..4280)
                     /gene="clpA"
                     /locus_tag="PL056_10765"
     CDS             complement(2025..4280)
                     /gene="clpA"
                     /locus_tag="PL056_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010234430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HPM47538.1"
                     /translation="MQLEKRLNNIILSAFYEAKNEKHEYVTAEHVFYSILFDADGLEI
                     LEQLQLNIDILKKEMKDYLKHNIPKSQAKKEVIQSEGFQLMLNNAASKAASSNRATVE
                     LGDVLVAIYDLEESFASYLLKNEGLERYDLLSVVSHGLTEKDDDDDEPSDDDEEISGI
                     EKRERKEISPEKLLKKYTTDLTEKATRNELDPIIGRADILKRTIQVLLRRKKNNPVHV
                     GEPGVGKTAITEGLAQMIVKGEVPHQLLNHTILKLDLSNVLAGTKYRGDFEERMKKII
                     AALEKVNDLILFIDEIHTLVGAGSAGSSSIDASNMLKPLLTEGKIKFIGATTYDEYRK
                     HFEKDSALTRRFQKIDIPEPTILETVEILRGLSKNYEQFHKVKYHDDALKAAAELSSR
                     YISERFLPDKAIDIIDEAGSINAMSNNPLKTIKKEQIEQVIAIATGKKESAVSVDKLK
                     ALKNLETEISSEIFGQTNAVENVVKAVKKSMAGFKPVEKPVASFLFAGPTGVGKTELA
                     RVLAKKLELPLHRFDMSEYQEKHTVAKLFGAPPGYVGYEEGGLLTEAIRKQSSCVLLL
                     DEIEKAHSDIYNSLLQIMDYATLTDSTGRKADFKNAIIIMTSNAGAKAIGKAMTGFGE
                     RKFESSALIDAVNRTFSPEFRNRLDSIVYFQPLNPEIVKNIVLKNIRELRIRMAEKKI
                     TINITDKAVAHLSTIGFSKEFGARETARIVEEKMEKLLLDEILFGKLRKGGTVNIDIN
                     KEELVIRNISK"
     gene            complement(4284..4598)
                     /gene="clpS"
                     /locus_tag="PL056_10770"
     CDS             complement(4284..4598)
                     /gene="clpS"
                     /locus_tag="PL056_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013239122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HPM47539.1"
                     /translation="MAGLTPEGTTKLKSRPASKVKKPSMYKVIMLNDNYTTMEFVIDI
                     LKKFFSKSPLEATEIMLNIHHNGQGIAGIYPYDIALTKVNQVKSEAKKSGFPLKCIIE
                     KE"
BASE COUNT         1180 a         1078 c         1001 g         1652 t
ORIGIN      
        1 aatctgcctg atcttcgttg ttgtcgtaaa tgcagttgtc gcaggcgtca ccgagaaggt
       61 ctccatctga atccttctga tccgaattgg gaactgaagg gcagttatcg gagctgtcaa
      121 gaacagtgtc accgtctgta tcaacatttt cacagacatt tcctgttccg tcactgtcat
      181 catcaagctg atcggcattg gcaactgcag gacagttgtc acatgcatca cccacaccgt
      241 caccatcaga atcaacctga ccgggattgt gtgtgccggg acagttatcc gattcatcag
      301 gtattccgtc accatccgga tcattcggct cacaggcgtt tcccaccccg ttggtgtcat
      361 agtcaagctg atttgagtta gctacttcgg ggcagttgtc gcacagatca ccgatactgt
      421 ctttatccat atcttcctgt ccggaattgt ggatatattt acagttgtca ttaaaatcga
      481 gaactccgtc tccatctgaa tcaagcggct cgcaggcatc tcctgtgcca ttgaaatctg
      541 tatcggtctg atcgttattt gctgtgtaga tacagttgtc acatacatca ccgaccccgt
      601 cagaatcggt atcaagctga tcagagttgg caatggtcgg acagttgtca acaagatctc
      661 tgaatccgtc gtggtctgtg tcaagaagga gtgtatcgtt ctggcaggcg tctccgacac
      721 cgtctttgtc cgtatcggtc tgtttaatgt tggcatgatt cgggcagttg tcacacacat
      781 caccttttct atcgttgtca gtatcgagct gatctgcatt ctttatctca gtgcagttgt
      841 ccttgcagtc catgatcccg tcaccgtctg tatcaacatc aagaacatcg catccgcaga
      901 tgccgggatc ggttttctcg ggattttcgg gacagagatc gataatgtcg gaatcttcgg
      961 tctcttcggt gtcagttact tcagtgtcaa ccggctcttc tgtatcattg acctgttctt
     1021 catctttttt tgattcttca tccttttcaa tgacagtgtc cttgtcggga actgtaacct
     1081 catcgtctgt aacaggaagt gaatctgacg aaccttcatc atcggtgatg ttgttggtgt
     1141 cggtaactcc tgtgttgtcg tcaacatttg tttttttccc gctgtcacag gatgcaaaga
     1201 aaatgattga aaagattaaa ataataaaag aaagcttctt cattgaattc ccccgaaata
     1261 aacagtatta taatttctta cagcacataa atattatttc tacctttatt tttgtcaagt
     1321 agtctataat ttgttgaaat ctatagtgtt tttttttgcc gcttttgaaa ttgtgtggac
     1381 aaactctttt ctgctgatga attcagctcc aagtgacttt aaatggtctg tttcgacctg
     1441 acagtcaaca atagggcact tagctttctg aagcacttgc ataaatgtta tgaaagccgc
     1501 ttttgaggcg ttttcgtata ttgagaacat gctttctcca aaaaaggcct ttccgagcat
     1561 cactccgtaa agtcctccgg caagattacc ggttcctgcc acccatgctt caacgctgat
     1621 ggcgtatccg agccggtgaa gctcattgta cgatttttcc atctcatctg taatccatgt
     1681 accttcctgt ctaacccgta cctcatgccg gcagcggctt attacttgag ggaacgcttt
     1741 gttgaaagtg atctcgaaaa cccctttttt gagcagtttg cccatgcttt ttgaaacatg
     1801 aactttttca gggaagatca caaacctcgg atcaggcgac caccagagta ttttattgcc
     1861 aagatagttg aactcgctgt tttccgagta ccacgggaat attcctgatc tgtaggcaag
     1921 aagaagccgc tctggagaaa gatctccgcc tattgcaaga agtccgtcgg gttcagaatg
     1981 ttcaacaggt ggaaaatcta aaatgtcagg agaaagggca tatatcattt tgaaatgttt
     2041 ctgatgacaa gttcttcttt gtttatgtca atatttacag tccccccttt cctgagtttt
     2101 ccgaaaagta tttcatcaag aaggagtttc tccattttct cttcaacgat ccttgctgtt
     2161 tctctcgcac cgaattcctt ggaaaaaccg atagttgaaa ggtgtgcaac cgccttgtcc
     2221 gttatattta tcgtaatctt cttttcagcc atccggatcc tgagctcacg gatgtttttc
     2281 aagacgatat ttttaacgat ctcgggattt agcggctgaa aataaactat tgaatcaaga
     2341 cggttcctga actcaggcga aaatgttctg ttcactgcat caataagtgc cgacgattca
     2401 aattttcttt cgccaaaacc agtcatggct ttgccgatag cttttgctcc tgcattggaa
     2461 gtcattataa ttattgcgtt tttgaaatca gcttttcttc cggtgctgtc tgtcagagtt
     2521 gcatagtcca tgatctgaag gagtgaatta tagatgtccg aatgggcctt ttctatttca
     2581 tcaagcagta acacgcacga tgactgtttt cttatcgcct cggtaagcag tcccccttct
     2641 tcatatccga catatcccgg aggtgctccg aaaagttttg caacagtgtg tttttcctga
     2701 tattcgctca tgtcgaaacg gtggagcggc agttccagtt ttttggcaag aacccgtgca
     2761 agttcagttt ttcctacccc cgtcggacct gcaaaaagaa aagatgcgac cggtttttca
     2821 accggcttga accctgccat gctctttttg accgctttga ctacattttc aacggcattt
     2881 gtctggccga atatttcact tgatatttct gtttcaaggt ttttaagtgc tttgagcttg
     2941 tcaacgctga ctgcactctc tttttttccg gtggcaatcg cgataacttg ctcgatctgc
     3001 tctttcttta ttgttttcag cgggttattg ctcattgcgt tgatcgagcc ggcttcgtcg
     3061 attatgtcaa tggctttgtc aggcaggaat ctttcggaaa tgtatctgct tgaaagttca
     3121 gcggccgctt tcaatgcatc atcatgatat ttaactttgt ggaactgttc ataattctta
     3181 gacaatccgc gaaggatctc aacagtttcc agaattgtcg gttcgggaat gtcaattttc
     3241 tggaatcttc ttgtgagggc ggaatctttt tcaaaatgtt ttctgtattc atcataagtt
     3301 gtcgcaccga tgaactttat tttcccttca gtcagaagcg gtttaagcat gtttgatgcg
     3361 tcaatgcttg agcttccggc actgcctgct cccacaagtg tgtgaatttc atcaataaaa
     3421 aggatgagat cattaacttt ctcaagagcg gcaattatct ttttcatcct ctcttcaaag
     3481 tcaccgcggt attttgttcc ggcaaggaca ttgctgagat cgagctttaa aattgtgtgg
     3541 ttaagaagct gatgaggcac ttcacctttc actatcatct gtgcaagacc ttctgttatg
     3601 gctgttttgc cgactcccgg ttctcccaca tgaacgggat tgttcttttt tcttcttaaa
     3661 aggacttgaa ttgttctttt taatatgtca gctcttccga tgataggatc aagctcattg
     3721 cgggtagctt tttctgtcag gtcggttgta tattttttta agagtttttc aggacttatc
     3781 tcttttcttt cccgcttttc aattcctgag atctcttcat catcatctga aggttcatcg
     3841 tcgtcgtcat ctttttctgt taaaccgtgt gaaaccactg ataacaggtc atatctttca
     3901 agtccttcat ttttaaggag atatgaagca aatgattctt caaggtcata aattgcaaca
     3961 aggacatctc cgagttcaac tgtcgcacgg ttggagcttg ctgcctttga tgcggcgttg
     4021 ttaagcataa gctggaatcc ctcagactga attacttctt tttttgcctg agatttcggt
     4081 atgttatgct tgaggtagtc tttcatctcc tttttgagga tatcaatgtt aagctgaagc
     4141 tgttcaagta tttccagccc gtccgcatca aaaaggatgc tgtagaaaac atgttctgct
     4201 gtgacatact catgtttttc gtttttcgct tcgtaaaaag cggaaagaat tatattgttt
     4261 agtctttttt caagctgcat cagttactcc ttttcgataa tacatttcag cggaaacccg
     4321 ctttttttcg cctcgctttt cacctgatta acttttgtca gtgcgatatc ataaggataa
     4381 attcctgcga tcccctgacc gttatgatgt atattaagca tgatctctgt cgcttcaaga
     4441 ggggacttgc tgaaaaattt cttcagaata tcaatgacaa actccattgt cgtatagttg
     4501 tcattaagca ttatcacctt gtacatcgac ggctttttca ctttgctcgc cggacggctt
     4561 ttgagttttg ttgtcccctc aggtgtaagg cctgccattt gtcaccccgt taaaaaaccg
     4621 ctttttttat cacaaactat tgattttgta aatatttcaa ttgtatgagt tgataataac
     4681 aacttgcaat ttctgtttaa tactttaaaa tttatccagt tttaaacata ttggagggaa
     4741 caatgaaaat aaaaacagtt tcttttttgt ttgttgtgtt tttctgcacg gcattaagcg
     4801 gggtaattca cggaaacatg ataggcggtg atccgtatga gcaatgggag gatggcggac
     4861 aggacttttt cgtaatgttc aactcaaata ttgataacaa actcgatctt t
//
LOCUS       DAOOYB010000211         4860 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_20292_length_4860_cov_13.167742, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000211 DAOOYB010000000
VERSION     DAOOYB010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4860
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20292_length_4860_cov_13.167742"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..800)
                     /locus_tag="PL056_10775"
     CDS             complement(<1..800)
                     /locus_tag="PL056_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013408733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HPM47540.1"
                     /translation="MIKPGEIQNKARESGVRDQQIEKDYVLSWILQGISQHEKLSKAI
                     VFKGGTVLKKVYFKDYRFSEDLDYTLLNNDVANELIFEWFAEVFKYIREEANIPLEII
                     NNNEHEDGGINFYIDYVGPLGGSGAGKKVKIDISRSEKLVFKPVMRDVFLGYTDQEEH
                     RLLCYSLEEVLVEKLRSVMQRMQARDFYDIWYLLEVHDMDAGFYIDEFKAKCENKGIN
                     SSGFHAKLEQRLPQYKKRWEKSMTDQIKNLPEFRMVLREVMRHLRGME"
     gene            complement(797..1618)
                     /locus_tag="PL056_10780"
     CDS             complement(797..1618)
                     /locus_tag="PL056_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015025050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin"
                     /protein_id="HPM47541.1"
                     /translation="MRVKNISTKSNLILTYFNQMDKDCFVYADAAEALPQTSDSALKE
                     LLSDMVKRGLLMRLKKGLYFIIPFEKDPDSFMPDWHIIADCLIKEADYYIGYYSALQI
                     HGLITQPSLKEQIVVSKQIRPSAIKIKGVDFQFIYHNENHFFGSKKIWIDSFNKVLCS
                     DLEKTFVDCLFKPDYAGGIVEVARAVYISKDKIDFNKLFKYAEEFKSQAVIKRLGFLL
                     ELLEIKTDIILKLNKLKTSSYVLLDTELPKSGKMIRRWSVQQNLETETIKSAVYT"
     gene            1855..3033
                     /locus_tag="PL056_10785"
     CDS             1855..3033
                     /locus_tag="PL056_10785"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HPM47542.1"
                     /translation="MENFKDKISSQLIELETNSRLRTLKTIEKRVGNHIWIDGVRYLN
                     CSSNDYLGIAGSISLKKEFLNWCAQNPDSLFTDLSSSSSRLLTGNSSIYDETERGIAQ
                     LYGRENCIIFSSGYHMNAGFFAAMYDKGDLIVADKLVHASIIDGIQLSRAEHIRYNHL
                     DYEHLRKILNENRTKYKSVVIVTESVFSMDGDIADLKELVNIAGSFDAQLFVDEAHAV
                     GCCGENGLGICEKTGTSKNIDFLAGTFGKALGGAGGFIVCDNVIKQFLINRCRSFIFT
                     TALPPINISWMNFILGKIAEMKKEREKLISASELFRNKLTESGFTTRVSSHIVPVITG
                     DDEKTVQLSAKMRSSGFYVPAIRPPTVPAGTSRLRFSLTAEFSKDEINSIVETLSGTN
                     "
     gene            3033..3698
                     /locus_tag="PL056_10790"
     CDS             3033..3698
                     /locus_tag="PL056_10790"
                     /inference="COORDINATES: protein motif:HMM:NF016209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF452 family protein"
                     /protein_id="HPM47543.1"
                     /translation="MKLSWLNNSGNGSLILFFSGWSVEPEHMGFLECSHFDVAMLHRY
                     DSFDIPDGIDFAKYDEIIAIAYSFGVFTGLTALSKINFSGEIFAVNGTMKPVDNRFGI
                     RKMVFDKTLANLNEETLDQFRKNMFADSPSYETFAKSSKFINNIDGLKKELEFIRDNS
                     EKGSSDLSKVQKVLISKSDRIIFSKNQVDFWNDFNYVLIDGGHFPFYGFKSWGEIVQL
                     CRQ"
     gene            3686..4438
                     /locus_tag="PL056_10795"
     CDS             3686..4438
                     /locus_tag="PL056_10795"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPM47544.1"
                     /translation="MQTVDKTRVKKKFLEALHSYDDHASVQRLMGLELVRELNNYSPE
                     KFGTVLEIGSGTGKLTKYLSREIRFKTIFCNDLFEEAYPYIVKWCKKENFLVCDGEDV
                     SKLPGNIDLLVSNATFQWFNDLPGFLHSVRSRLNDNAIAGFTTFGPSQFKETKEITGK
                     CLEYVVRKDLEEQIQDKYKVLYFNEWEETLKFASVKRVLKHISSTGVSGVSSSLPSMD
                     FETFQTKYKEKFFDGEMYSLTYHPQIWILKAL"
     gene            4417..>4860
                     /locus_tag="PL056_10800"
     CDS             4417..>4860
                     /locus_tag="PL056_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47545.1"
                     /translation="MDIESSLIKGCLICGSKTADNGLFCSECGKFALTVERRCGRCGA
                     FTLDPSKPDCNSCRSKKIFFDTIFSCYIYSGPVSSVISKMKYGKIESFAVVLGTFLAM
                     NVPADIVNGRTVIFPPMSFFHKFSRSFNQAQIIADRVASIHKLELD"
BASE COUNT         1489 a          967 c          921 g         1483 t
ORIGIN      
        1 aactccattc cccttagatg tcgcattact tctcttagaa ccattctaaa ttccggcaag
       61 ttcttaattt ggtcagtcat agatttttcc cagcgttttt tgtactgtgg caaacgctgt
      121 tcaagtttgg catggaaacc agaagagttg atgcctttgt tttcgcattt tgctttgaat
      181 tcgtcaatgt agaaaccggc atccatatca tgaacttcca gcaaatacca tatgtcgtaa
      241 aaatcacgag cctgcatgcg ctgcataacc gatcgtaatt tttccaccag cacttcttcc
      301 aaagaatagc aaagcagccg gtgttcttcc tgatcggtat atccaagaaa aacatcacgc
      361 atgacaggtt taaataccag tttttcactt cttgaaatat caatttttac ttttttacca
      421 gctcctgagc caccaagtgg gccgacataa tctatataaa aattgattcc accatcttca
      481 tgctcgttgt tgttgattat ttctaacgga atattcgctt cttctcttat gtatttgaac
      541 acctctgcaa accattcaaa tattaattcg ttggcaacat cgttatttag caaggtataa
      601 tcaaggtctt cagaaaatct ataatctttg aaatagacct tcttcaggac agttccacct
      661 ttaaaaacga tagctttaga aagtttttca tgctgagata ttccttgtag aatccatgat
      721 agaacatagt ctttttcaat ctgctggtcc cgtacaccgg attcacgggc tttgttttgt
      781 atttctcccg gttttatcat gtatagactg ccgatttaat agtttctgtt tcaagattct
      841 gctgaacact ccagcgacga atcatctttc ctgactttgg cagttctgtg tccagaagca
      901 cataagaaga tgttttcagt ttgtttaatt tcaatataat atctgttttg atttcaagta
      961 attctaataa gaacccaagt cgtttaataa ccgcttgtga cttaaattct tcagcatatt
     1021 taaataattt gttaaaatcg atcttgtctt ttgatatata gaccgctctt gccacctcga
     1081 caattcctcc cgcataatcc ggcttaaaca aacagtcaac aaaggtcttt tccaagtctg
     1141 aacataacac cttgttaaag ctgtctatcc atattttttt agaaccgaaa aaatgattct
     1201 cattgtgata aataaattga aaatcaactc cctttatttt aattgctgaa ggacggatct
     1261 gctttgatac aactatctgc tcttttaaag aaggctgagt aattaagccg tgtatctgta
     1321 aagcagagta atatcctata taataatcag cttcttttat cagacaatct gctatgatat
     1381 gccaatcagg cataaaagaa tccggatctt tttcaaaagg aataataaaa tataatcctt
     1441 tttttaatcg catcaacaac cctcttttaa ccatgtcgct gaggagctct tttaaagcac
     1501 tgtcacttgt ttgaggcaat gcctctgctg catctgcata gacaaagcaa tccttatcca
     1561 tttgattgaa gtaggttaaa atcagattgg acttagttga aatgtttttt actctcatag
     1621 tgcacaccgc cacttttaac ctagtgcgat tatacgaatt gcaatcgcat aatcaagtaa
     1681 aagctatgtg ctatagtaaa aaggtcagct caaaactgac ttaagtgata tgaaacataa
     1741 catcaggttc aacggcagaa tctatgagaa acaacttcat taatgtaaat ccttgacaaa
     1801 tacaggcatt gaaagaatat tgctttctag gggtccgtac actcagaggt ttttatggaa
     1861 aatttcaaag acaaaatatc gtctcaactc attgaacttg aaacaaattc acggcttaga
     1921 actttaaaaa ccattgaaaa aagagttggc aatcacatct ggatcgatgg cgtaagatat
     1981 ctgaactgtt cgtcaaatga ctatcttggc attgccggca gcatttcgct taaaaaggaa
     2041 tttcttaact ggtgtgctca aaacccggat tctcttttta ctgatctttc aagcagttcc
     2101 tcccgccttc tgacagggaa cagctcaatt tatgatgaaa ccgagcgtgg aattgcacaa
     2161 ctttatggaa gagaaaactg cataatattc tcaagcggtt atcacatgaa cgccggcttt
     2221 tttgccgcaa tgtatgataa aggcgatctg atcgttgccg ataaacttgt tcatgcaagt
     2281 atcattgacg gaattcagct ttcaagggct gaacatatcc ggtataacca ccttgattat
     2341 gaacatttaa gaaagatcct gaatgaaaac aggacaaaat ataagtcagt cgtaattgtt
     2401 acagaatcgg ttttcagcat ggatggcgac attgcagatc tgaaagaact tgtcaatatt
     2461 gccggatctt ttgacgctca gctttttgtt gatgaagcac atgcggtcgg ctgctgcgga
     2521 gagaacggac ttggaatatg cgaaaagaca ggaacatcta agaatattga ttttctggca
     2581 ggaactttcg ggaaagcact cggaggagca ggcggcttca tcgtttgcga caatgtgatc
     2641 aagcagtttc ttattaacag atgccgctca tttattttca caacagccct tcctcctata
     2701 aacatttcgt ggatgaattt catcttggga aagatcgctg aaatgaaaaa agaaagggaa
     2761 aaacttatat ctgcaagtga actcttcaga aataagctta ctgaatccgg ttttaccaca
     2821 agggtttctt cacacatagt tccggtaatt acaggtgatg atgaaaaaac ggtgcaactt
     2881 tccgcaaaaa tgagatcatc gggtttttat gtccctgcca tcagaccgcc gacagtaccc
     2941 gccggaactt cccggctgag attttctctc acggcagaat tttctaaaga tgagataaat
     3001 tcgatagttg aaacactttc aggaaccaat taatgaaact ttcatggctc aataattcag
     3061 ggaacggttc gctcatactt ttcttttcgg gctggagcgt tgagccggaa catatgggtt
     3121 ttcttgaatg ctcgcacttt gatgttgcga tgcttcacag atatgactct tttgacattc
     3181 ctgacgggat cgacttcgca aaatatgacg aaattattgc aattgcttat tctttcggcg
     3241 tttttacagg tttaaccgcc ctttcaaaga taaatttctc aggagagata tttgcagtaa
     3301 acggaacaat gaaaccggtt gacaaccgtt tcggcataag aaaaatggtt tttgataaaa
     3361 cccttgcaaa cctgaacgaa gagaccctgg atcagttcag aaaaaatatg ttcgcggata
     3421 gtccttcata cgaaacattc gccaaaagct ctaaattcat caacaatatt gatggcttaa
     3481 aaaaagaact cgaattcatc cgtgataata gtgaaaaagg gagcagtgat ctttcaaaag
     3541 tacagaaagt tctcatttca aagtcggaca gaataatatt ttcaaagaat caagttgatt
     3601 tctggaacga tttcaactat gtattgattg acgggggtca ttttccgttt tacgggttta
     3661 agagctgggg ggagatagtt caattatgca gacagtagac aaaacgagag taaaaaagaa
     3721 atttcttgaa gcacttcatt cttacgatga tcacgcttca gttcagcggc tgatggggct
     3781 tgagcttgta agggaactta acaattactc tcctgaaaaa ttcggcactg ttcttgagat
     3841 cggttcagga acagggaaac tgacaaaata tctgagcaga gaaatcaggt tcaaaactat
     3901 tttctgcaac gatctttttg aagaggcata tccttacatc gtcaaatggt gcaaaaaaga
     3961 gaattttctt gtctgtgacg gtgaagatgt ttccaaactt ccgggcaata ttgatctcct
     4021 tgtttcaaat gcgacatttc aatggttcaa tgatctgccg ggatttctcc attcggtccg
     4081 gtcacggctc aacgacaatg cgatagccgg atttacaact ttcggcccat cgcagtttaa
     4141 agaaacgaag gagataacag gaaaatgtct tgaatatgtt gtcagaaaag atcttgaaga
     4201 gcagattcag gataaataca aagttcttta tttcaatgaa tgggaggaaa ctctcaaatt
     4261 cgcttctgta aaaagagttc tcaaacatat ttcctcaact ggagtttcgg gggtttcaag
     4321 ctctcttcct tctatggact ttgagacttt tcagacaaaa tacaaagaaa aattctttga
     4381 cggtgagatg tattctctca cctatcatcc gcagatatgg atattgaaag ctctctgata
     4441 aaggggtgtc tcatctgcgg ttcaaaaact gcagataacg gactgttctg cagtgaatgc
     4501 ggaaaatttg cacttactgt tgaaagaaga tgcggcagat gcggagcttt cacactcgac
     4561 ccttcaaagc cagactgcaa ttcgtgccgc agtaaaaaga tattttttga cacgatcttt
     4621 tcctgttaca tctattccgg cccggtatca agtgtcatat caaaaatgaa atatggaaaa
     4681 atcgagtcct ttgccgtagt tctcggaact tttcttgcaa tgaatgttcc tgctgacatt
     4741 gtgaacggga ggactgtgat ctttcctcca atgagctttt tccacaaatt ctcaagatct
     4801 ttcaatcagg cacagatcat cgccgacaga gttgcttcaa tacataagct cgaacttgac
//
LOCUS       DAOOYB010000212         4859 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_20300_length_4859_cov_9.894671, whole genome shotgun sequence.
ACCESSION   DAOOYB010000212 DAOOYB010000000
VERSION     DAOOYB010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4859
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20300_length_4859_cov_9.894671"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="PL056_10805"
     CDS             complement(<1..231)
                     /locus_tag="PL056_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007061925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPM47546.1"
                     /translation="MTVKFAIVGCGRILKKHLEAIRDIEKAELVAVCDIISEKAEIAG
                     KNANVPWYTCYNEMLSKHPETDVVTILTPSGMH"
     gene            complement(228..677)
                     /gene="nrdR"
                     /locus_tag="PL056_10810"
     CDS             complement(228..677)
                     /gene="nrdR"
                     /locus_tag="PL056_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HPM47547.1"
                     /translation="MKCPFCGSFKDKVIDSRSTKDDSEIRRRRECEDCGGRYTTYERI
                     EEVMPIVKKKNGEREPFDRNKIKKGLLISCQKRPVSAEVIDQIIDTVEKTVQSYEKKE
                     IESSAIGDLVMQQLKKIDKVAFIRFASVYKEFREPTDFITEAKEVHK"
     gene            complement(700..1992)
                     /gene="glyA"
                     /locus_tag="PL056_10815"
     CDS             complement(700..1992)
                     /gene="glyA"
                     /locus_tag="PL056_10815"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HPM47548.1"
                     /translation="MLNLKYIPSVDPEVAEIIEKEAKRQEEGLELIASENFTSNAVME
                     AQGSLLTNKYAEGYPKKRYYGGCEVVDEVEKLAINRACELFGAEYANVQPHSGSQANM
                     GVYFAVLNPGDTVLAMNLDHGGHLTHGSPVNFSGKLYKIIPYGVSPVDGKIDYAELER
                     LAVENKPKLILAGASAYPRVFDFPRIREICDKVGAMMMVDMAHIAGLVAAGVHPSPVP
                     YADFVTSTTHKTLRGPRSGLILAKKKYEKELNSSVFPGMQGGPLMHIIAAKAICFKEA
                     MSEEFKVYQQKVVKNAAAMADEFNKMGYHLVSKGTDNHLMLIDLRNKGKTGKITEKAL
                     DKAHITVNKNMVPFDDQSPFITSGIRVGTPFLTTRGLNEEDVRLVARLMDKIIMNIKT
                     NEFSPKIGIDSSVIDDSVIEEVRAEVKKICQKYPLIRK"
     gene            2126..3562
                     /locus_tag="PL056_10820"
     CDS             2126..3562
                     /locus_tag="PL056_10820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HPM47549.1"
                     /translation="MTDIIIIAIYFIAVLAAGHFFSAGNESVKDYFLAGRKMGWMMIG
                     ISVMVTSFSTLNFVSVTGEVFSQGLYVLISVPMFFIVIYPVLKWFIPFYKSADVTSAY
                     EYLEIRFDRSVRTAAGIMFILWKTVWMSVSLYAAGVALASVSSIPLETVILLCGITAL
                     IYTVSGGMKAVMVTDMMQFFVIVIALLGIIFISVSSFDGGMSELFSTIADSGRLTPFD
                     PFDSSIFSLSPDVRISIFSTFTGVAVAFLSRYGVDQVVVQRYFSAKNESEVKKGIILN
                     AFAASGVIVSVAVFGLILKGFSIRHGINGNFLETLKIFMASLPAGVSGLLSAALFAAM
                     MSSIDSGINSCSCAFFTDIMPVIGKERSFSIAKARIVSLLIGASAIIFAFPIGRMGPI
                     FETVNKVVNGLGAPLLALFVCGAVIKKIDAKSALAGFISGIIASILVIVFVRNVSLHY
                     YAVINFILTSALIWLIYFLRKTTKTLQI"
     gene            3618..4829
                     /locus_tag="PL056_10825"
     CDS             3618..4829
                     /locus_tag="PL056_10825"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM47550.1"
                     /translation="MYLHRMIEENIVKAIEFFPVIALSGARQTGKSTMLKKILGEKFR
                     YISFDDYTIRKMAKESPSLLLKNFQEDLIIDEIQYAPEILSAIKMCVDSGEKRRFVLT
                     GSQQFNLMKNLKESLAGRVLIMNLYPMSVSEKKGNGKKKHWLTEVLEKGQPPSEKTET
                     TVEPAAELIRGGLPGLLEKPDVFINSYIESYIKTYIERDIPEMYAGVDPLKLATFLKL
                     LAPLSSQLINKSQLGRDIGVSSPTANRWIDWFSAGSIYSMTQPYIGNVIKRLTQMPKG
                     TLFDTALICHLMQISSKETLITHPLLGSIFESRVLHEFKAVISSAMIPADIYHWRTSS
                     GAEVDIVIEKEGKLYAFECKWKSDIRKKDLSGLIAFRETYKDKVRFSAVITPSGETLE
                     IDDSIYRISLF"
BASE COUNT         1322 a         1000 c          987 g         1550 t
ORIGIN      
        1 atgcattcca cttggagtca gaattgtaac aacatcggtt tcagggtgtt ttgaaagcat
       61 ttcattgtag catgtgtacc acggcacatt tgcgtttttg cctgcaattt cagctttttc
      121 gctgataatg tcacagactg cgacaagttc cgccttttca atgtcacgga ttgcttcaag
      181 atgttttttt aagatccttc cgcaaccgac gatcgcaaac ttaacagtca tttatgaacc
      241 tctttggctt ctgtaataaa atcagtgggt tccctgaatt ctttatagac cgatgcaaag
      301 cggatgaaag caactttgtc tattttcttc agctgctgca ttacaagatc tcctatagct
      361 gagctctcga tctccttttt ttcataggac tgaacggttt tttcaaccgt atctatgatc
      421 tgatcgataa cttcagctga aacgggtctt ttctgacagc ttatcagaag tccttttttg
      481 atcttgttcc ggtcaaacgg ttccctttct ccgtttttct tttttacgat cggcataact
      541 tcttcgatcc gttcgtaagt tgtgtatctt ccgccacagt cttcacattc ccttcttctt
      601 cttatctctg aatcatcttt ggtagatctg gagtcaataa ctttgtcttt aaatgagccg
      661 cagaatggac atttcatggt tttttccgtt ttttaacagt tactttctga tgagaggata
      721 tttctgacag atctttttaa cttcagcgcg cacttcttct atcacggaat catcaattac
      781 agacgaatca atgccgatct taggcgaaaa ctcgtttgtt ttgatgttca ttatgatctt
      841 gtccataagc cgtgcaacaa ggcggacatc ttcttcgtta agtcctcttg ttgtcagaaa
      901 tggtgttcca accctgatac cgcttgtaat gaatggtgac tgatcgtcaa aaggaaccat
      961 gttcttgttc acagtgatat gtgccttgtc gagtgctttt tcagtgatct tacctgtttt
     1021 acctttgttg cgaaggtcaa tgagcatcag gtggttgtct gtcccttttg aaaccaggtg
     1081 ataccccatt ttattaaact catctgccat ggcagctgca tttttaacaa ctttctgctg
     1141 ataaaccttg aattcttcgc tcatcgcttc tttaaaacag atcgcttttg cggcaattat
     1201 gtgcatcagc ggtccgccct gcattcccgg gaaaacagag gaattcagtt ctttttcata
     1261 ttttttcttt gccagtataa gtccgcttct tggacctctc agggttttat gtgtcgtcga
     1321 agttacgaaa tctgcataag gaacagggct cgggtgaact cccgccgcga caagacctgc
     1381 aatgtgggcc atatcgacca tcatcattgc cccgacttta tcacagatct ccctgattct
     1441 gggaaaatca aaaactctcg gatatgccga agcacctgca agaatgagct tcggtttgtt
     1501 ttcaactgca agccgctcaa gttcggcata atcaattttt ccgtcaaccg gtgaaacacc
     1561 gtaaggaatt atcttgtaaa gttttcctga gaaattaacg ggacttccgt gagtcaggtg
     1621 tccgccgtga tcaaggttca tcgcaagaac tgtgtcaccc ggattaagaa ctgcgaaata
     1681 tacacccata tttgcctgag aaccgctgtg gggctggaca tttgcatatt cagcaccgaa
     1741 aagttcacat gcacggttaa tggcaagttt ttcaacttca tccactactt cgcaaccgcc
     1801 gtaatatctt tttttcggat aaccttcggc atatttgttg gtaagaagag atccctgtgc
     1861 ctccatgaca gcattgcttg tgaagttctc gcttgcaata agctcaagcc cctcttcctg
     1921 tcttttcgcc tctttttcga taatttcagc cacttcaggg tcaacacttg gaatgtactt
     1981 aagattcagc atatcatttc ctccaaaaga agatagttaa caaaaaacat attgagcata
     2041 atacattaaa attttttttg caaaaaaaca ctttttgaca aagggtcggc aatcggtaat
     2101 ctcaacatga aaaaggagga tttccatgac cgatattatt atcattgcga tatacttcat
     2161 tgcggtgctt gccgcaggac acttcttttc agccggaaat gagtcggtaa aggactattt
     2221 tcttgccggc aggaagatgg gatggatgat gatcgggatc tccgtgatgg tcacttcatt
     2281 cagcacactt aattttgttt cggtgacagg tgaagttttc agtcagggtc tttatgtcct
     2341 gatttcggtt ccgatgttct ttattgtgat ataccctgtc cttaaatggt tcatcccttt
     2401 ttataaaagt gctgatgtca cttcagctta tgaatatctc gaaatcagat ttgacaggtc
     2461 agtgagaact gccgcaggaa ttatgttcat tctctggaaa acagtatgga tgtcggtttc
     2521 cctttatgcg gccggggttg ctcttgcttc ggtaagttca attcctcttg aaacggtcat
     2581 ccttctttgc gggataacag cacttatcta tacggtgagc ggaggaatga aagcggtcat
     2641 ggtgacagat atgatgcagt tttttgttat agtgatcgcc cttttgggga taatcttcat
     2701 ttcagtttcg agttttgacg gcggaatgtc agagctgttt tcaacaattg ctgattcagg
     2761 gagactgaca ccgtttgatc cttttgacag ctcaatattt tcattaagtc cagatgtgag
     2821 gatctccatt ttcagcactt ttaccggtgt tgcagtggca tttctttcaa ggtacggggt
     2881 cgatcaggta gttgttcaga gatatttcag tgcaaaaaat gaaagtgaag ttaaaaaagg
     2941 gataatttta aatgcatttg ccgcctcggg agtcattgtt tctgttgctg ttttcggact
     3001 gatcttaaag ggcttcagca tacgccacgg gataaacgga aatttccttg aaactcttaa
     3061 aatattcatg gcttcacttc ctgccggagt ttccggactg ctctctgccg ccctttttgc
     3121 cgcaatgatg tcaagcattg attcaggaat aaattcatgc agttgtgcat ttttcactga
     3181 tattatgccg gtcattggga aagagcgttc tttttcaatt gccaaggcaa ggatcgtttc
     3241 tctattaatc ggtgcatctg ccattatttt tgcatttcct atcggcagaa tgggtccaat
     3301 atttgaaacg gtcaataaag ttgtgaacgg gctcggagca ccacttcttg ccctttttgt
     3361 ctgcggagcc gtaataaaga aaattgatgc aaaaagtgct cttgccggtt tcatttcagg
     3421 tattattgcc agcattctcg taattgtttt tgtcagaaat gtttcactcc actattacgc
     3481 agtcataaat ttcattctga catctgcctt gatatggctg atttattttt taaggaaaac
     3541 gacgaaaacc ttgcaaattt aatgtcaggc gttaaaatta caaggtgatt tggagactgt
     3601 ttctttgagg tgaagagatg tatttgcata ggatgattga agaaaacatc gtaaaagcta
     3661 tcgaattttt tcctgttatt gcattaagcg gagcgagaca gacaggaaag tcaacaatgt
     3721 tgaaaaaaat tctgggagag aagttcagat atatttcttt tgatgactat acaataagaa
     3781 aaatggcaaa ggaatcacct tcgctcctcc taaagaactt tcaggaggat cttatcattg
     3841 atgaaattca atacgcaccg gaaatattat ctgcaataaa aatgtgtgtt gattcaggtg
     3901 aaaagagacg attcgtgctt accggctcac agcagtttaa tcttatgaaa aaccttaagg
     3961 aatctcttgc cggcagagtt ctgataatga acctttatcc tatgagtgta agcgaaaaaa
     4021 aaggaaatgg taaaaagaaa cactggctta cagaagttct tgagaaagga cagccccctt
     4081 cagaaaaaac tgaaacaacc gttgagccgg cggcagaact gatcagaggc ggtcttcccg
     4141 gacttcttga aaagccggat gtttttatta attcttatat tgaaagctat ataaaaacat
     4201 acattgaaag ggatattcct gaaatgtatg cgggagtaga tccgttgaag cttgccactt
     4261 ttcttaaact tcttgcacct ctttcgtctc agttaatcaa taaaagtcag ctcggaagag
     4321 atatcggagt ttcttctccc acagcaaacc gctggattga ctggttttca gccggctcaa
     4381 tatacagcat gactcagcca tatataggaa atgtaataaa aagacttact caaatgccga
     4441 aaggaacact atttgacact gcgttgatct gccacttaat gcagataagc tccaaagaaa
     4501 ctcttatcac tcaccccctt cttggatcaa tatttgaaag cagagttctc catgaattca
     4561 aagcagtcat atcttctgca atgatccctg ccgacattta tcactggcgg acatcttcag
     4621 gtgctgaagt tgatattgta atagaaaaag aggggaagct ttatgccttt gaatgcaaat
     4681 ggaaaagtga tataagaaaa aaagatcttt ccggactcat tgctttcaga gaaacatata
     4741 aagataaagt cagattctca gcagtcatta ctccgtcagg agaaacttta gaaatagatg
     4801 attccattta ccggatatct cttttctgac attgaaagag tcaaccaatc tgaaaactt
//
LOCUS       DAOOYB010000213         4850 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_20347_length_4850_cov_8.434202, whole genome shotgun sequence.
ACCESSION   DAOOYB010000213 DAOOYB010000000
VERSION     DAOOYB010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4850
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20347_length_4850_cov_8.434202"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4466)
                     /locus_tag="PL056_10830"
     CDS             complement(<1..4466)
                     /locus_tag="PL056_10830"
                     /inference="COORDINATES: protein motif:HMM:NF014466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HPM47551.1"
                     /translation="MKKILIILMIFSPFLLFSDLIEIVPKPVNNIVGTDKIVVRGDYD
                     SGNSEIRIAVSQNGNIRYYSPIILHTELDGYYVHGVELFNGLNTIKLESKTTNDWPGV
                     VVAEKQVTYYSTGTDAAKSLWGRIANDGIFKNEEQRAIFFYPGTRFEVNSDLAEPMSL
                     STPDLSGLFLSRISELGFEEQKYGYIKNQILGNENIGRKDGIDTKAPADSYQKCWSSK
                     ETINFMMDEAQNANVNILIFVYWGDQNETLREDLPYNSGILTENNLDQLDNFWKIYSA
                     PTFPETCNYKIKFDSNFCIEYSGRPRFMSSFPLDYPDAPAPTYDALEATREAVYCQCN
                     SDSDCNESTHYCCFGTCRKKTCNSDEDCHEIIFPDRISFGKCIEANGVKKCSPQNFYN
                     TPLWFHYYEKSDCLDDEIFTQSEYLNSGMAVPIDRLSDSIDQLFKAAADRNMLVVPSI
                     EFNMFHAFWVEFPHNLDPVRHTISQMIAKYGNHPNWLKIYDRNGIPRKAILISQALSL
                     QLGKYLDYNGVETLVLEGDYVSAMDMLANEFSTEDNKIGFLIDNTAMPADYSMSQFFT
                     PKASDLMNMDSLLSVSTFGLPFNQQVLVNSVDNAKILTWSDNISVNGYPDQVEPIEDH
                     FVNKAKEWLENWKMYGQNIPMVANVIPGYDDRWRGIIMGNSTSGGFNIFGDNYLWHMR
                     NADITFMYNTAGISLSIWNGYGEGYVWVPYRKRSFSEYIENNQSVYTVDPVYQNGILK
                     IPESFLDLKFMFEEDTINPLYEDQVIYDNYNYAKYLFSLDQDGDYVSDNNDNCPDTPN
                     PDQFDSDNDGVGDACDICPGTYNPVVDYSLESEFISGNEGALAKGLCRENEKWLFFIK
                     RKVCSMQPDSDLDGIGDACDYDSSAGGGFANSRITNAKPKTIIPSITSAPVSLSRTYN
                     HYAEINLTMPSGSGREKFYCNDISLSDLYRGSSCNAAVHYCAVDYINYNERDRWGQQG
                     FCSTRNAAMPFPIKHNYGYSHASDDFSPESMASWQSRISVSDNLNLFWPAFTGSSDPN
                     DDTARKPVINSNASFKLVFGENGTIWNWRRDWYEWNDCPTTPSDTKCQSLKNAAAYNV
                     SHTMYYTLSTSILPVAAGTPNENIPRYLINVNNETVINPAYFPATNTSVNARAARYST
                     EPMELNFYSRSILPPVPPETIEIPEIVYCPSCYWNLPIEDILDEQGMPEAFSINHIGR
                     WLTGKDENGNYMMNSQHLYFPENMTIFSEMTEHAMVAVRTVETPATGETEYQLLLNTS
                     ENGADWEFLGNISNWDSEITSIRAIVSNDSGMYFIAGTDSQGQYGQYLFRVSVESVEP
                     ETTYSLNNLGLTGIGDDTLENEKLVSVEGALFVIGADSAGAGTRTFKSASGEAPFTEI
                     TGLSPASRKIYNLKAAGKYIFLTGGMNANNGSMTDMWRFDTETGIWEQIPITLNGDFR
                     KVITQFVDGKLVMANPVMTGNLTHPAFEINPNITNINDLAASMNYVEIPVTEA"
     gene            complement(4476..>4850)
                     /locus_tag="PL056_10835"
     CDS             complement(4476..>4850)
                     /locus_tag="PL056_10835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47552.1"
                     /translation="YNVYGENKFVEVILTDIDKPEYVEYEVTKHMENAEYWGPSKLQG
                     KLAIYIESDPYDDIGCFYRFDKKKSYCPAENIFSTSPDNLMGFNTFWGKWHLWKGIGR
                     TAAFMRDWECYCKETGVCPFDE"
BASE COUNT         1386 a         1029 c          885 g         1550 t
ORIGIN      
        1 gcttcagtta caggtatttc aacatagttc atgcttgcag caagatcatt tatgtttgta
       61 atatttggat taatttcaaa tgcagggtga gtgagattgc ctgtcataac gggattagcc
      121 attacaagtt tgccatcaac aaactgtgtg attactttcc tgaaatctcc attgagagta
      181 attggtatct gttcccatat tccggtttca gtatcaaatc tccacatatc ggtcatactg
      241 ccgttgtttg cgttcattcc gccagtgagg aagatatatt tccctgcagc tttaaggtta
      301 tatatctttc ttgaggcggg tgataatcct gtgatctctg tgaaaggggc ttcacccgat
      361 gcagacttaa atgttcttgt tcccgctcct gctgaatcgg ctcctattac aaagagtgcc
      421 ccttcaactg aaacaagttt ctcattttca agtgtgtcat cacctattcc ggtgagtccg
      481 aggttgttaa gggaatatgt tgtttccggt tcaacacttt caacggaaac tctgaacagg
      541 tattgtccgt actgaccctg tgaatcagtt ccggcgatga aatacattcc tgaatcattt
      601 gaaacgattg ctcttattga ggttatctct gaatcccagt tactgatatt gcccagaaat
      661 tcccagtcag ctccgttttc acttgtgtta agcagaagct gatattcagt ttctcccgtt
      721 gccggagttt ccaccgttct cacggcaacc attgcgtgtt ctgtcatttc agagaaaatt
      781 gtcatatttt caggaaaata gagatgctgg gaattcatca tgtaatttcc gttctcatct
      841 tttcccgtca gccatctgcc gatatggttt atggagaaag cttcgggcat tccctgctca
      901 tcaaggatat cttctatcgg aaggttccag taacatgaag gacaataaac tatttcaggg
      961 atctcaattg tttcaggcgg aaccggaggc agtatggatc tggaatagaa attcagttcc
     1021 atcggctcag tcgaatatct tgccgccctt gcattgacac ttgtgttcgt tgcagggaag
     1081 tatgcggggt ttattacagt ttcattgttg acattaataa ggtatctggg gatattttca
     1141 ttcggagtac ccgctgcgac gggaagaatg ctcgttgaaa gtgtgtaata cattgtgtgt
     1201 gagacattat atgccgccgc atttttcaga ctttgacatt tagtatctga aggtgttgtc
     1261 ggacagtcgt tccattcata ccagtctctt ctccagttcc agatcgttcc gttttcaccg
     1321 aagacaagct taaaggatgc gttgctgttg ataacaggtt ttcttgcagt gtcgtcattg
     1381 ggatcactgc tcccggtgaa agctggccag aaaaggttta aattgtcgga aaccgaaata
     1441 cggctctgcc agcttgccat agattcagga gaaaaatcat cgcttgcgtg gctgtagccg
     1501 taattatgtt ttataggaaa aggcatcgca gcattccttg tactgcagaa gccttgctgt
     1561 ccccatctat ctctttcatt ataatttata taatcaacag cacagtaatg caccgccgca
     1621 ttacatgatg agcctctgta caaatccgaa agacttatgt cgttgcagta aaacttttca
     1681 cgaccactgc ccgaaggcat agtaagattt atttcagcat agtgattata tgttctggaa
     1741 agagatacgg gagcggaggt tattgatgga attattgttt tcggcttggc attggtgatc
     1801 ctgctgtttg caaaaccgcc accggcagag gaatcatagt cacatgcatc accgatgcca
     1861 tcaagatcac tgtcaggctg cattgaacac acttttcttt taatgaaaaa gagccatttt
     1921 tcattttccc ggcacaggcc ttttgcaaga gcaccttcat taccggaaat aaattccgat
     1981 tcaagactgt aatcaacaac cggattataa gttccggggc aaatgtcaca tgcatcgcca
     2041 acaccgtcat tatccgaatc aaactgatct ggattgggag tgtctgggca gttatcattg
     2101 ttgtcagaaa cataatctcc gtcttgatca agtgaaaaaa gatacttcgc gtaattatag
     2161 ttgtcataaa taacctgatc ttcgtataaa ggattaattg tatcctcttc aaacatgaat
     2221 tttaaatcaa gaaatgactc gggaatcttt aaaataccgt tctgatacac aggatcaact
     2281 gtataaactg actggttgtt ttcaatatat tctgaaaacg atctttttct ataaggcacc
     2341 catacatatc cttctccata tccgttccat attgaaagac ttattccggc tgtattatac
     2401 ataaatgtaa tatccgcatt tctcatatgc cacagataat tatcaccaaa aatattgaat
     2461 ccgccggaag tgctgtttcc catgattatt cctctccaac gatcatcata tcccggaata
     2521 acattagcta ccatggggat attctgacca tacattttcc agttctcaag ccattctttt
     2581 gctttgttta caaaatgatc ttcaatcggt tcaacttgat cagggtatcc attcacagaa
     2641 atattatcag accatgttaa tatcttggca ttgtcaactg aattaactaa aacctgctgg
     2701 ttaaaaggca atccgaaagt gctgacagat agaagactgt ccatattcat caaatccgat
     2761 gctttgggtg taaaaaactg actcattgaa taatctgccg gcatagcagt attgtcaatt
     2821 aagaatccta ttttattatc ttctgttgaa aattcatttg ccaacatgtc catagcactt
     2881 acataatcac cctccaacac taatgtttcc acaccgttat aatccaaata tttcccgagc
     2941 tgcagactca gtgcctgaga aatgagaata gcttttcgcg ggattccgtt tctgtcataa
     3001 atctttaacc agttagggtg attaccatat ttagctatca tttgtgagat tgtatgcctt
     3061 accggatcca gattatgcgg gaactccacc cagaatgcat gaaacatatt aaactcaatt
     3121 gatggaacaa caagcatatt cctgtcagca gcagctttaa aaagttgatc tatagaatca
     3181 gagagcctat cgattggaac agccattccg ctattcaaat attcactctg cgtaaaaatc
     3241 tcgtcatcta aacaatcaga tttttcataa taatgaaacc acagaggagt attataaaaa
     3301 ttttgtggag agcatttttt gacaccgtta gcttctatgc attttccaaa tgatattcgg
     3361 tcaggaaaaa taatttcatg gcaatcttca tctgaattac atgtcttttt tctgcaggtg
     3421 ccaaaacaac aataatgtgt tgattcattg cagtcagaat ctgaattgca ctgacaatac
     3481 acagcttctc ttgtggcttc aagagcatcg tatgttggag ctggagcatc cgggtaatct
     3541 aaggggaaag aagacataaa gcgtggtcgt ccagaatatt ctatgcaaaa gttgctatca
     3601 aattttattt tgtagttaca tgtttccgga aatgtgggtg cagaatatat tttccaaaaa
     3661 ttatctaatt gatcaaggtt gttctcagta agaattccac tgttatatgg aagatcttca
     3721 cgcaatgttt cattctgatc accccaatat acaaatatca atatattcac attggcattt
     3781 tgcgcctcat ccatcataaa atttatagtt tcttttgatg accagcattt ctgataagaa
     3841 tctgcaggag ctttcgtatc aatcccgtct tttctgccaa tgttttcatt tcctaatatc
     3901 tgatttttaa tataaccgta tttttgttct tcaaaaccca gttccgatat tcttgaaaga
     3961 aacagtccgc ttagatcagg agtgcttaac gacataggtt ctgctaaatc agaattaact
     4021 tcgaatctgg tccctggata gaaaaatatt gcacgctgtt cctcattttt aaaaatccca
     4081 tcattcgcaa ttcttcccca cagagatttt gcagcatcag ttccggtaga gtaatatgtc
     4141 acctgcttct ctgcaacaac aactcccggc cagtcatttg tagttttact ttcaagctta
     4201 atagtgttca acccgttaaa aagttctacc ccgtgaacat aatagccgtc cagttctgta
     4261 tgtaagatta tcggtgaata ataccggata ttaccgtttt gagaaactgc gattcttatt
     4321 tcgctgtttc cggaatcata atcacccctg acaacaattt tgtctgttcc tacaatgtta
     4381 ttaactggtt taggcactat ttctattaaa tcggaaaaca acaagaatgg ggaaaaaatc
     4441 attaaaataa ttaatatttt cttcattacc aactcctact catcaaacgg gcagacaccc
     4501 gtctccttac aataacattc ccagtcccgc atgaaggcag ctgttctgcc tattcctttc
     4561 catagatgcc atttgcccca gaaggtgtta aagcccatta aattatccgg ggaggttgaa
     4621 aaaatatttt ctgcagggca gtatgacttc ttcttgtcaa atctgtagaa acagccaata
     4681 tcatcataag gatcagattc aatgtaaatt gcaagttttc cctgtagctt actcggcccc
     4741 cagtattccg cattttccat atgttttgta acttcatatt ccacatattc cggcttatca
     4801 atgtccgtca gaataacttc cacaaattta ttctctccat atacattata
//
LOCUS       DAOOYB010000214         4847 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_20368_length_4847_cov_10.137730, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000214 DAOOYB010000000
VERSION     DAOOYB010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4847
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20368_length_4847_cov_10.137730"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="PL056_10840"
     CDS             complement(<1..706)
                     /locus_tag="PL056_10840"
                     /inference="COORDINATES: protein motif:HMM:NF015100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HPM47553.1"
                     /translation="MSRYVISLSPHEKGTYTVNTVMWGVVIALMPSFLASVYFFGVRA
                     VAVTAVAVATCIGVEYLIQKFMLKCEITALDGSAAITGILLAFNLPSSIPLWEVVVGA
                     IFSIGIAKMVFGGLGKNPFNPALAGRAFMLASFPVDMTTWPIPVKTAMMWTMGKAVDG
                     TTSATPLGMLKEGIKSNSNITDIIAGSPQSFDYFNLFIGNIGGCIGEVSALAIILGGI
                     YMLYKKIITWHIPVFYL"
     gene            complement(703..2040)
                     /gene="rsxC"
                     /locus_tag="PL056_10845"
     CDS             complement(703..2040)
                     /gene="rsxC"
                     /locus_tag="PL056_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HPM47554.1"
                     /translation="MKLKTFKIGGVHPPENKMAASTPIEALAIPKRVSIPLSQHIGAP
                     AVPVVKKGDDVKVGTLIAKGESFISANIHSSVSGRVFAIDTVTDTTGYKVKAVIIDVE
                     GDVWDESIIRTPELAKEIKLEPADIVAKIKSSGIVGLGGACFPTHVKFMVPEGKKVDT
                     LVINAVECEPYLTADHRIMLERTEEILVGIEIMKKALKVSRAIVGIEANKPDAIAKME
                     KVASDFPGTEIQPLKVQYPQGAEKQLIKAALNREVPSGKLPLDVGCVVNNVGTALAIY
                     EAVQKNKPLFERVVTVTGKKIKRTANFLVRIGTPVEALVEAVGGIPEGTEKIVNGGPM
                     MGKAVVSPEISVTKGTSGIIFFDKSEAKRKKVSNCIRCAKCVTVCAMGLQPYLLEKLA
                     EKNNWEELEKLSVTDCIECGSCSYTCPSARPILDLIRLGKANVNRIRWERSKK"
     gene            complement(2051..2962)
                     /locus_tag="PL056_10850"
     CDS             complement(2051..2962)
                     /locus_tag="PL056_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010664617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HPM47555.1"
                     /translation="MDGIILYSVISLGTVAAVSAIVLFVIAKKFKVEEDPRIDMIAEL
                     LPGANCGGCGYPGCRGLAEALVKAADAGSIAGMNCAPAGGEQMAKIGKALGLEVTALE
                     PTVAVLLCGGTHEKAPKKTDYDGPSKCSIAHTVSCGDKGCPYGCLGLGDCVTVCAFGA
                     MYMDEKTGLPVIIEEKCVSCGACVKACPRKLIEIRPQGRKSRRVWVNCRNTEKGATAM
                     KVCKAACIGCGKCVKECPEKVQAITLDNFLAYIDPKKCIACGKCIVVCPTKAISATFE
                     PPAPKPKPVEEPVKSEKKEEVKEQQQI"
     gene            complement(2971..3399)
                     /locus_tag="PL056_10855"
     CDS             complement(2971..3399)
                     /locus_tag="PL056_10855"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HPM47556.1"
                     /translation="MTVSKVTHNGIVISVDGVKASVKIEVQSACSGCHAKGFCSAADM
                     AEKIIETVSEESVEPGESVIVEMDQRLGFKAVMFAFLIPFILLVATLFAAWNISGSES
                     VAAFSSIGILVPYYLGLVVFKTYFKKNFVFTCRKISNISQ"
     gene            3655..>4847
                     /locus_tag="PL056_10860"
     CDS             3655..>4847
                     /locus_tag="PL056_10860"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM47557.1"
                     /translation="MIVKRDLGAFLKKVSGLFPAVIVTGPRQSGKTTFLKYTFPEAEY
                     VTFDDPVMQDFAKNDPNGFIAKFSSRQIILDEIQYVPELFSYLKMDIDQNREVCGKWL
                     LTGSQQFNMMKEISDSLAGRIAILSLFPFSQNELSSAGKNLEIDKILWNGAYPEVVLK
                     PEIREIWLASYIRTYIERDVRQLINVADLSLFQTFIGLVAANHSQELNVAGISRDCGA
                     AQPTIKRWISILEASFIIYLLRPFHVNIGKRLVKTPKLYFIDSAIPAYLTKQGSSESL
                     FNGAMGGAFFEGFIVVETLKIIKNSSANAELYFWRSNDGMEIDLIIEFAGKYHAVEIK
                     KTQTPTAKHAESIEKFITLFKEKTGDSFVVCNIPQTKPLTANVSAVPWQKYFKWVEEI
                     LGFNQ"
BASE COUNT         1356 a         1084 c          948 g         1459 t
ORIGIN      
        1 caagatagaa tactggaata tgccatgtta ttattttttt ataaagcata tatattccac
       61 cgagaatgat cgctaaggca cttacttcac cgatacatcc tccaatatta cctataaaaa
      121 ggttgaaata gtcaaaactc tgaggagaac cggcaattat atcagtaata ttgctgtttg
      181 attttattcc ctctttcaac ataccaagag gagtggccga tgtagttccg tcaactgcct
      241 ttcccattgt ccacatcatg gccgtcttta cagggattgg ccatgtcgtc atgtcaactg
      301 gaaaactcgc aagcatgaat gctctgcctg caagagccgg attgaacgga tttttaccca
      361 gaccgccgaa aaccattttg gctataccga ttgagaatat ggcacctaca actacttccc
      421 ataatggaat gctgcttgga agattgaacg caagaagaat tcctgtgatt gccgctgatc
      481 cgtctaacgc agttatttcg catttgagca tgaatttctg tattaaatat tcaactccta
      541 tgcatgttgc aacagcaacc gctgtaacag cgacagctct tacaccgaaa aaatatactg
      601 atgcaagaaa tgacggcata agtgcgataa caacgcccca cataactgta ttaacggtat
      661 atgtcccttt ttcatgcggg gaaagcgaaa ttacatatct gctcattttt tactcctttc
      721 ccatctgatt ctgttaacat ttgccttgcc gagccgtata agatcgagta tcggtcttgc
      781 acttggacag gtataactgc agcttccgca ttcgatgcag tctgtgacgc tgagtttttc
      841 aagttcttcc cagttgttct tttctgcaag tttttcaagc agatatggtt gaagccccat
      901 cgcacataca gttacacatt ttgcacatct gatacagttg ctgacctttt ttctttttgc
      961 ttccgacttg tcaaagaaaa ttatgccgct tgtccctttt gtaactgaaa tttcaggact
     1021 tacaacagct tttcccatca tgggtccgcc atttacgatc ttttcagttc cttccggaat
     1081 tccgcccact gcttcaacga gtgcttctac cggtgtgccg atacgaacaa gaaaattggc
     1141 agttcttttt atttttttgc cggtaacagt tacaactctt tcaaaaagcg gcttgttctt
     1201 ctgcactgcc tcataaatcg caagtgccgt tccgacatta ttaacgacac atccgacatc
     1261 aagaggaagt ttgcctgacg gaacttcacg gttcagagcc gcttttataa gttgtttttc
     1321 agcaccttga ggatactgga ctttgagcgg ctgtatttca gttcccggaa aatcagatgc
     1381 aaccttttcc atttttgcta tggcatcggg tttgtttgct tctattccga cgatcgctct
     1441 gcttacttta agagcttttt tcattatctc gatgccgaca aggatctctt cagtccgttc
     1501 aagcattatt ctgtggtcgg cagtaagata tggctcgcat tcaacagcat ttatgacaag
     1561 agtatcaact tttttccctt ccggaaccat gaattttaca tgggtcggaa aacaggcacc
     1621 gccaagaccg actataccgc ttgatttgat ctttgcaacg atgtcagccg gctcaagctt
     1681 tatttcttta gcaagctccg gagttctgat aatgctctca tcccacacat caccttcgac
     1741 atcaattatg accgctttga ctttatatcc ggttgtgtca gtaacagtat cgatggcaaa
     1801 aacccttccg cttaccgaag aatggatgtt tgccgaaatg aaagattccc ctttcgcaat
     1861 taaagttccg acttttacat cgtccccttt tttaacgacc ggaactgccg gtgcgccgat
     1921 atgctggctt aacggaatag atactctttt cggaatggca agggcttcta tcggtgttga
     1981 agccgccatt ttattttcgg gcggatgaac tccgccgatc ttaaaagttt tgagtttcac
     2041 aacactctcc ctatatctgt tgctgttctt tgacttcttc tttcttttcg cttttcacgg
     2101 gctcttcaac cggtttaggt ttgggagccg gcggttcaaa agttgccgat atcgcttttg
     2161 tgggacatac aactatgcac tttccacagg cgatgcattt tttcgggtcg atatatgcaa
     2221 gaaaattgtc aagagtgata gcttgaactt tttcaggaca ttcctttaca cattttccgc
     2281 aaccgatgca ggcagctttg cacactttca tagcagtcgc ccctttttcg gtattacggc
     2341 agttgaccca gaccctgcgt gattttctgc cctgtggtct tatttcgatg agctttctag
     2401 gacatgcttt tacacaggca ccgcacgaaa cacatttttc ttcaatgata acaggcagac
     2461 ctgttttttc atccatatac attgctccga atgcgcatac tgtaacgcag tcaccaagac
     2521 ccagacatcc gtagggacat cctttgtccc cgcatgaaac tgtgtgtgct atggaacatt
     2581 ttgaaggtcc gtcataatcg gtctttttcg gggctttttc atgtgttccg ccacaaagaa
     2641 gaacggcaac tgtcggttcc agagcagtca cttcaagtcc gagtgctttt cctatttttg
     2701 ccatttgttc gcctccggca ggagcacagt tcatgcctgc aatacttcct gcgtccgccg
     2761 ctttcacgag tgcttccgca agaccgcgac atcccggata tccgcatccg ccacagtttg
     2821 caccgggaag aagttcagcg atcatatcga tcctcggatc ttcttcaacc ttgaactttt
     2881 ttgcgataac aaaaaggacg atggctgaaa ccgccgctac tgttccgagc gagatcacag
     2941 aatagagaat aattccgtcc acaattcacc tcactggctg atattactaa tctttctaca
     3001 ggtaaataca aaattctttt taaaatatgt cttaaatacc actaatccga gataataggg
     3061 gaccagaatc ccgatcgatg aaaatgccgc aacagattca gaccctgaaa tgttccatgc
     3121 ggcaaaaaga gttgcaacaa gaaggatgaa aggaattaaa aatgcgaaca ttaccgcttt
     3181 gaaaccgagc cgctgatcca tttcaacgat cacactctcg ccaggttcaa cagattcttc
     3241 agaaacagtt tctataatct tttcagccat atcggccgca gaacagaacc cttttgcgtg
     3301 acatcccgag catgcactct gcacttcgat cttcacagat gctttaacgc cgtccacact
     3361 tatcactata ccgttatgag taactttgga aactgtcatc tttttcgcct gcaaaaaaca
     3421 cacctctatc tactatttag atgttgcttt gtcaaatata ttggtattag aaagaaacaa
     3481 tttacaaaca gattaatttg acaaaaaccg catggaccat aaaagatatc aaatgaccta
     3541 tgtttcaggt cataaaactt caaaaaacat gaatataaag caatgaatct aaatacttga
     3601 caattataac attggatgtt agatttgtca gagatttttt ttgaggggtt gatcatgatc
     3661 gtaaagagag atttgggagc atttttaaaa aaggtgtcgg ggctgtttcc tgcggttatt
     3721 gtgaccggtc cgagacagtc aggtaaaacg acttttctta aatacacttt ccctgaagcg
     3781 gaatatgtca cttttgatga tcctgtaatg caggattttg cgaaaaatga tccaaacggg
     3841 ttcattgcaa aattttcgtc acggcagata attcttgatg aaattcaata tgttccggaa
     3901 cttttttcat atttaaagat ggatattgat caaaacaggg aagtttgtgg aaaatggctg
     3961 ttaaccggtt ctcagcagtt taacatgatg aaagaaataa gtgattctct tgccggaagg
     4021 attgccatac tcagcctgtt tccattcagc cagaatgagt tgtcttctgc cggaaaaaat
     4081 ttagaaattg acaaaattct ctggaatgga gcatatccgg aagttgtttt gaagcccgaa
     4141 ataagagaga tttggctcgc ttcttacatc agaacttata tcgaaaggga tgtgagacag
     4201 ttgataaatg ttgcagacct ttcacttttt cagactttta tcggactggt tgcggcaaat
     4261 cactctcagg aacttaatgt cgcaggaatt tcaagagact gtggtgctgc ccaaccgaca
     4321 ataaaaagat ggatatcaat acttgaggca tcattcatca tatatcttct cagaccgttt
     4381 catgtgaata ttggaaaacg gctagtgaaa accccgaaac tttattttat tgattctgca
     4441 attccggctt atttaacaaa acagggaagt tccgaatcac tgttcaacgg agcaatgggg
     4501 ggtgcttttt ttgaaggatt tatagtcgtt gaaacattga agatcattaa aaacagttca
     4561 gcaaatgcag agctatattt ctggcggtca aatgacggaa tggaaattga ccttatcatt
     4621 gaattcgcag gaaaatatca tgcagtagaa atcaagaaaa cccagactcc gactgcaaaa
     4681 catgctgaat caattgagaa attcataaca ctttttaaag aaaaaaccgg tgattctttt
     4741 gtcgtctgca atattcctca gacaaaacct ctgacagcaa atgtatcggc agttccatgg
     4801 cagaaatatt tcaaatgggt tgaagaaatc ctcggattta atcagga
//
LOCUS       DAOOYB010000215         4840 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_20411_length_4840_cov_4.694462, whole genome shotgun sequence.
ACCESSION   DAOOYB010000215 DAOOYB010000000
VERSION     DAOOYB010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4840
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20411_length_4840_cov_4.694462"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="PL056_10865"
     CDS             complement(<1..656)
                     /locus_tag="PL056_10865"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HPM47558.1"
                     /translation="MNEIKTGLVLSGGGAKGAYQVGAIKALVEYGFEFDMISGASIGA
                     LNGAVLASSGSFSEGAAKLEFLWKEICKLNVLQPNLNTDISLIRILSNLGLNIGPIKY
                     LSKINRIYDSAKNKGLIDVLNTDVLKITDSLIKIAFADEDALLSNDPIKKIVSDAVDI
                     KKLKDGIPLFVSVYESEGRAIKDFMTWLLSEKTGLFETKDSEFIRVNGLSEDLATDVI
                     L"
     gene            complement(673..1548)
                     /locus_tag="PL056_10870"
     CDS             complement(673..1548)
                     /locus_tag="PL056_10870"
                     /inference="COORDINATES: protein motif:HMM:NF020442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FRG domain-containing protein"
                     /protein_id="HPM47559.1"
                     /translation="MKIANIHEYLKEIKKLGKENDSNTFCSLFSRGEPEDYKDTACVP
                     GIFRKQKFIENEHNMFRDFIIANPDEFNNDRLTIDKLTKMQHYSLPTRLLDVSENALV
                     SLFMACESKENNDSIVWIFRVPKEKIKYYDSDTVSVIANLAKRPNFIFPKGFEKISEN
                     ITNTEHRKKYLEDFNKIDDIKYLLHDIKEDKPYFKDEIWPEHLYSVICVKPRQNNRRI
                     IRQQGLFLLFGNNKKDKLKHAEIKDSGITKEKIIIDARWKKQIIEDLALMGITKATVY
                     PDMDKVAEYLKEKYS"
     gene            complement(1545..1835)
                     /locus_tag="PL056_10875"
     CDS             complement(1545..1835)
                     /locus_tag="PL056_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47560.1"
                     /translation="MKCRAIIKNIKFCKSMAEGKTTTCVQIEFYDIVNWEKEQIVFRD
                     FAPEDLSRYSDKWFKCEFDHSLLYQFMKSSQLLWVEIEPEILKANILEITGK"
     gene            complement(1825..2733)
                     /locus_tag="PL056_10880"
     CDS             complement(1825..2733)
                     /locus_tag="PL056_10880"
                     /inference="COORDINATES: protein motif:HMM:NF025356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HPM47561.1"
                     /translation="MNDIQRFFDDKKEIIEKLPNLPDKFIVDFANGIDVAKDHVRVKR
                     SRGGFFDRFCDSIITGKDHRRQNEINMCLIDGLEGSLRWLTEVTESLSLTNIAVIKAF
                     ESIDKIQSSQSKIIDYSIETRKSLNELNELFQSRVKTLEKEIKRIDFEQKAKRHMDSV
                     LDKWKASKLNDFSIAGRVAAVLEELRWGDFGYFITKVINDQRTRLQFLADLENKLIIR
                     TREELGGNEAFETKFIMKSDKRESELIDALAYLSNWANPEESPIIYLATQFPEEYPRQ
                     IAHIYNPATFSKHVVSEVFEGGNHEM"
     gene            complement(2735..4717)
                     /locus_tag="PL056_10885"
     CDS             complement(2735..4717)
                     /locus_tag="PL056_10885"
                     /inference="COORDINATES: protein motif:HMM:NF012570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HPM47562.1"
                     /translation="MELLKKEILKQKDKTEGLKKDKKDNDDIKEILNFISSGAKPKEK
                     YEGPEEIFEFLKILNDLVRICNEYDVDFPFTDYDEIHELPVIEIEFSSLKELENPGGV
                     LTLLDTPGPNESKQKHLKTMLKEQMKKASAVLAVLDYSQLKSEADEEVREYLREISEV
                     SKGRLFALVNKFDEKDRNCDDESSVKKFISQDLLKGILEEENNIYPVSAKLGFLANKA
                     LYELEINGCLPDIKENPWVKDFAKEAFGSRWENKLNDVDEAKECAEALWKESLFQKPL
                     EEIIEHSYNKSAIFAIDSSASKLLDTASRIENFLNTRDGALSKDIKELQKQIDSLQND
                     IERVEESEKNIKKSSGKMLKKLDEEIEEMFENSKDNLQKVLDSFFKEGKLLEKKHFKE
                     AEKDGDFFKNIGMRFKKDAKNNHRWNPQNPVINFYGEKKAAEDFVNNIQDNIQGIFVN
                     TEIEIKKNVDKGLKKFQKDFKTEIEKNIENVKDSIKNRLDEFGFNLNIEVPSSKKLEI
                     NVSAEDVFENVIKDKSGWRDKDGGWAKISRFFSFINSDWGREYVNRFEIDMNIIKNSC
                     FETTKKSFSGIENAIEKTVKVPLNESIEDFFSTFKTTIENIRSDFLQGIKDKQKNKKE
                     QEELVKSFKKLIKKLGKTKDHAALLKNEVETVINGG"
BASE COUNT         1358 a          916 c          660 g         1906 t
ORIGIN      
        1 agaatcacat ctgtggcaag atcttcagac aaaccattaa ctctaataaa ttcagagtct
       61 tttgtttcaa aaagacctgt cttttcggac aacaaccaag tcataaaatc tttaatggct
      121 cttccttcag attcataaac tgaaacaaat aaaggaatgc catcttttag tttcttaata
      181 tcaacagcgt cactgacaat ctttttaata gggtcattgc ttaaaagggc atcttcatca
      241 gcgaatgcaa tttttattag ggagtcagtt atttttaaaa catcagtgtt gagaacatct
      301 attaaccctt tgttttttgc agaatcatat attctattta tttttgacag atattttatt
      361 ggaccaatat ttaatccaag gttggaaagg attctaatta aagaaatgtc tgtgtttaag
      421 tttggctgaa ggacattaag tttgcagatt tctttccaca aaaactcaag ttttgcggca
      481 ccttctgaaa aagatccaga tgaagcaagc actgcaccat ttaaagcacc tatacttgca
      541 ccggaaatca tatcaaattc aaatccatat tcaaccaggg ctttaattgc tccaacctga
      601 tatgctccct ttgcaccgcc cccggaaaga acaagccctg ttttaatttc attcatataa
      661 aaccccttgt ttttatgaat atttttcttt taagtattca gcgactttat ccatgtcagg
      721 atatacggtt gcctttgtga tgcccattaa tgccaaatct tcaattattt gttttttcca
      781 tcttgcatca attataattt tctctttagt aatgccagag tctttaattt cagcatgttt
      841 taacttgtct tttttattgt tgccaaacaa aagaaacaag ccctgctgcc gaataatccg
      901 tcggttattt tgcctaggct tcacacatat aactgagtat aaatgttccg gccatatttc
      961 atctttaaaa tacggcttat cttcttttat gtcatggaga agatatttaa tatcatctat
     1021 cttgttaaaa tcttcaagat acttttttct gtgttcagtg tttgttatgt tttcagaaat
     1081 tttctcaaac ccttttggaa atataaagtt aggtcttttt gcaaggttag caatgacact
     1141 gaccgtgtcg ctgtcataat atttaatttt ttctttagga actcgaaata tccagactat
     1201 ggaatcgttg ttttccttac tttcgcaagc cataaaaagt gagacaagtg cgttttcaga
     1261 aacatccaga agccttgtgg gcaaactgta gtgctgcatt tttgtaagtt tgtcaatggt
     1321 caacctgtca ttgttaaact catcggggtt agcaataatg aaatctctaa acatattatg
     1381 ttcattttca ataaattttt gttttctgaa aattccagga acacatgcag tgtccttata
     1441 atcctctggc tctcctcttg agaaaagaga gcaaaaagtg ttggagtcat tttcttttcc
     1501 tagttttttg atttctttca ggtattcatg aatgtttgct atcttcattt tcctgttatt
     1561 tccaatatgt ttgcttttag gatttcaggc tcaatctcga cccagagcaa ctgactgctt
     1621 ttcataaatt gatacaataa agaatgatca aattcacatt tgaaccactt gtctgaatat
     1681 cttgataaat cttcaggggc aaaatctcta aaaactattt gttctttttc ccagtttaca
     1741 atatcgtaaa attcaatctg aacacatgtg gttgtttttc cctcagccat gcttttacaa
     1801 aacttaatat ttttgattat tgctctacat ttcatgattt cctccttcaa atacttcaga
     1861 cacaacatgc ttagaaaatg ttgccgggtt atatatatgg gctatttgtc ttggatattc
     1921 ttcaggaaat tgagtggcta aatatattat ggggctttct tcaggatttg cccagttcga
     1981 caaataggca agtgcatcaa ttaattcaga ttctctttta tctgacttca taataaattt
     2041 tgtttcaaat gcttcgttac cccctaattc ctctcttgtt cttattatga gtttgttttc
     2101 gagatctgct aagaattgca aacgggttct ttgatcatta attacttttg taataaaata
     2161 tccaaaatcg ccccacctta gttcttcaag aacagcagcc actcttcctg caattgagaa
     2221 atcattcagt tttgaagctt tccatttatc taaaactgaa tccatatgtc tttttgcttt
     2281 ctgttcaaaa tcaattcttt ttatctcttt ttccaaagtc ttcactctgc tttgaaacaa
     2341 ctcattaagt tcatttaacg attttcttgt ttcaattgaa taatctatta tttttgactg
     2401 acttgattgt attttgtcaa tactttcgaa tgcttttatt acagcaatgt ttgtcaaaga
     2461 aagagattct gtcacttcag tgagccacct aagacttcct tcaagtccgt ctatcagaca
     2521 catatttatt tcattttgtc ttcgatgatc cttcccggta ataattgaat cacaaaaacg
     2581 gtcaaaaaaa ccgcctcttg atcgtttgac tcttacatgg tcttttgcaa catcaattcc
     2641 gttcgcaaaa tcaacaatga acttatcagg caaattcggc aatttttcta tgatttcttt
     2701 tttatcatca aaaaatcttt gaatgtcatt catctcaacc tccgttgatt acagtctcta
     2761 cttcattttt taaaagagcc gcgtggtctt ttgttttgcc gagctttttt ataagttttt
     2821 taaaagactt taccagctct tcctgttctt ttttgttttt ctgcttatct tttattccct
     2881 gtaggaagtc acttctaatg ttctcaatgg ttgttttgaa agttgaaaag aagtcttcta
     2941 ttgattcgtt taaggggact tttactgttt tttcaatggc attttcaatg ccgctaaaag
     3001 atttcttcgt tgtttcaaaa caactatttt ttataatatt catgtcaatt tcaaaacgat
     3061 tgacatactc tctgccccaa tctgaattta taaagctaaa aaatctggaa attttcgccc
     3121 atccaccatc tttgtccctc cagcctgact tgtctttaat aacattctca aacacatctt
     3181 cagctgaaac attaatttca agtttttttg aagatggaac ttctatattg agattaaaac
     3241 caaactcatc aagtctattt tttatagaat cttttacatt ttcaatgttt ttttcaattt
     3301 ctgttttaaa atctttctgg aattttttta gtcccttatc aacattcttt tttatttcaa
     3361 tttctgtgtt tacaaagatc ccctgaatgt tgtcttgaat attattaaca aaatcttcag
     3421 cggctttttt ttcaccgtaa aaatttataa ctggattttg aggattccat ctatgattat
     3481 tcttagcatc ttttttaaat ctcattccaa tatttttaaa aaaatcacca tctttctcag
     3541 cttctttaaa atgctttttc tcaagcaact ttccttcctt aaaaaaacta tcgagaactt
     3601 tttgaagatt gtcttttgag ttttcaaaca tttcttcaat ttcttcatcc agttttttaa
     3661 gcattttgcc ggatgatttt tttatgtttt tttcactttc ttcaactctt tcaatgtcat
     3721 tttggaggga atctatttgt ttttgcagct cttttatgtc ttttgaaagt gccccgtctc
     3781 tcgtatttaa aaagttttca attcttgatg ctgtgtcaag aagttttgat gctgatgaat
     3841 caattgcgaa aatggctgat ttattataag aatgttctat tatttcttct aatggtttct
     3901 gaaacaaaga ttctttccat aaagcttccg cacactcctt tgcctcatca acatcgttta
     3961 atttgttttc ccatctgctt ccaaatgctt ctttggcaaa atcttttacc catggatttt
     4021 ctttgatatc aggcagacag ccattaattt ctaactcata gagagcttta tttgcaagaa
     4081 aacccaattt tgctgaaaca ggatatatgt tgttttcttc ttcaagaatc ccctttaata
     4141 agtcttgtga tataaatttt ttaacacttg attcatcatc gcaatttcta tctttttcat
     4201 caaatttatt aacaagagcg aatagtcttc cttttgaaac ctcagatatt tctcttaagt
     4261 attctctaac ttcttcgtct gcctcagatt ttaactgaga ataatctaaa acagctaaaa
     4321 cagcagacgc ttttttcatc tgctccttca acatagtttt taaatgtttt tgctttgact
     4381 cgtttggtcc tggagtatct aaaagtgtca atactccacc aggattctcc agttctttta
     4441 atgatgaaaa ctctatctct attacaggca attcatgaat ttcatcatag tctgtaaaag
     4501 ggaaatcaac atcatattca ttgcatattc tgaccaaatc attaagtatt tttaaaaact
     4561 caaatatttc ttctggtcct tcatattttt ctttgggctt tgctccggaa gatatgaaat
     4621 tcaaaatttc tttaatatca tcattatcct ttttatcctt ttttaaaccc tctgttttgt
     4681 ctttttgttt tagtatctct ttttttaaca attccaacaa attgcttatg ggtttatagt
     4741 tatgaaaatt caagataggg acggttaatc cttatctgtg tcttataagt gttggaattg
     4801 ccgtcatcgg tctgtttctg ttaggcatca cctcgttccc
//
LOCUS       DAOOYB010000216         4833 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_20450_length_4833_cov_8.225617, whole genome shotgun sequence.
ACCESSION   DAOOYB010000216 DAOOYB010000000
VERSION     DAOOYB010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4833
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20450_length_4833_cov_8.225617"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..469)
                     /gene="arfB"
                     /locus_tag="PL056_10890"
     CDS             complement(44..469)
                     /gene="arfB"
                     /locus_tag="PL056_10890"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017315628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HPM47563.1"
                     /translation="MITITNNQGRKISIPMSELEFSATRSTGPGGQHVNKTSSAVQLR
                     FDIESSSLPLFVKSKLLTMNDHRITSQGEVIIKVTDDRSQIQNRETAIERFKELIIKA
                     AFVPKKRKPTKPTKSSVENRLKSKKIRSEVKKMRSFKEL"
     gene            complement(592..1794)
                     /locus_tag="PL056_10895"
     CDS             complement(592..1794)
                     /locus_tag="PL056_10895"
                     /inference="COORDINATES: protein motif:HMM:NF016083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF401 family protein"
                     /protein_id="HPM47564.1"
                     /translation="MIWIQVLIPILLMVILLAKKIRLELVLPAGAICMALISGVDLPD
                     IGSEWLNSFINRRSADLFIIIMSVMLLGELMEKGKMFGRMSAIFLKIFRDNRIAAAAS
                     AAFIGFLPMPGGSLISAPFVDKLLPGYEQKEKMAVNYWYRHIWEYFFPLYAGLAWIIT
                     VMKVPATDLFIYQGAMTVVMASAGLPILLKHKKQEITELATAENPLKLLVISWPILLA
                     VGLSIVFSLDLPWAVLISLIAFIIKERHLAVYIKPFVMKKRTVYVIFLVTGMVFFDSI
                     VRSSGVATGVYEALKGSNPAVFVFLVPMICGVLTGITIGFVSISFPVIYPFLVVNGVV
                     DMNYLLLGFIGGFMGVMLSPMHLCLIVTTDFFKTTLKDVYPYIIKAGVLTLAGGVLYY
                     LGMKLTCL"
     gene            complement(1837..2418)
                     /locus_tag="PL056_10900"
     CDS             complement(1837..2418)
                     /locus_tag="PL056_10900"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012582951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPM47565.1"
                     /translation="MEKNFFVHETAIIDDNVRIGEGTKIWCFSHILKNTTIGKNCVFG
                     QNCVVGANVKVGNGVKVQNNVSVYEGVEIEDDVFLGPSMVFTNVINPRSFIERKEEFR
                     KTLMKKGCSVGANATIVCGATVGRYAFIGAGSLVTKDVPDFALVYGVPARQHGWVGVS
                     GNKLCFDSSDIATDKSDGKKYILKNGRVSEYQG"
     gene            complement(2418..3368)
                     /locus_tag="PL056_10905"
     CDS             complement(2418..3368)
                     /locus_tag="PL056_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007041352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPM47566.1"
                     /translation="MKKNFALIGAAGYIAPRHMKAIKETGNDLLAMLDPNDSIGIIDS
                     YFPEAEFFVEFERFDRHIDKLRRRENRIDYVSIASPNYLHDAHIRLALRNMSDAICEK
                     PLVLNPWTVDNLKIIEKETGHHVNTILQLRLHPTIAALKKKIDEDPSDKVYDVDLTYI
                     TSRGRWYDVSWKGDMSKSGGMATNIGIHFFDMLQWIFGYVQKNIVNVQERDVAAGFMQ
                     FEKARVRWFLSINENMLPKQAIEKGKKTFRCITIDGEELEFSEGFTDLHTVSYQKILA
                     KEGFSLEDSRTAVEIAFNIRNACPVGLRGDYHPFLKSWRR"
     gene            complement(3368..4633)
                     /gene="tviB"
                     /locus_tag="PL056_10910"
     CDS             complement(3368..4633)
                     /gene="tviB"
                     /locus_tag="PL056_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018060480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Vi polysaccharide biosynthesis
                     UDP-N-acetylglucosamine C-6 dehydrogenase TviB"
                     /protein_id="HPM47567.1"
                     /translation="MKEKKICIIGLGYVGLPLAVEFGRKYDTVGFDINKKRLEELRKG
                     VERTLEVEPAKLKQAQKLVYTSDINDIKNCNFFIVTVPTPVNEHKQPDLTPVVKGTET
                     VAKALKKGDIVIYESTVYPGCTEEVCVPILERISGLKFNVDFFCGFSPERVNPGDKVH
                     TVTTIKKITSGSTPESAEEIDQLYKTIITAGTHKASSVKVAEAAKVIENSQRDINIAF
                     VNELALIFEKLGIDTIDVLEAAGTKWNFLPFRPGLVGGHCIGVDPYYLTHKAQAVGYN
                     PEVILAGRRINDNMGIYVANKVVKLMIQKNKKIKGSRVLVLGITFKENCPDIRNSRVI
                     DVISEFREFGCIVDVVDPMADHHEVLEEYGVELSNFSECLKNDYDAVVLAVSHEKFRS
                     YDLKGFVDKNTVMYDIKGFLDRNIVTGRL"
     gene            complement(4630..>4833)
                     /locus_tag="PL056_10915"
     CDS             complement(4630..>4833)
                     /locus_tag="PL056_10915"
                     /inference="COORDINATES: protein motif:HMM:NF012506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HPM47568.1"
                     /translation="PVNVDGMIGAVLLALDIPGELANGIFMISRVPGLIAHYYEEKKT
                     QKPMRNIDQKSAVYTGKERRSIK"
BASE COUNT         1331 a         1140 c          898 g         1464 t
ORIGIN      
        1 gaacacgcgc aaaataatac tgtacagccc gatttttgtc aatttatagt tctttgaaac
       61 tgcgcatttt tttcacttcg cttctgatct tttttgattt gagccggttc tcgacggagc
      121 tttttgtcgg ttttgtcggt tttcgcttct ttggaacgaa agccgctttg attatcagtt
      181 ccttaaacct ttcaattgcg gtttccctgt tctgtatctg ggaacggtcg tcagtgactt
      241 ttatgatcac ttcgccttga gaggttatcc tgtggtcatt cattgtcaga agctttgatt
      301 tcacgaacaa aggaagggaa gagctctcaa tatcgaaccg gagctgaacc gcactgctgg
      361 tcttgttgac atgctgaccg cccgggccgg ttgaacgggt cgcggaaaat tcaagctctg
      421 acataggaat tgaaatcttt ctaccctgat tgttcgtgat cgtgatcatc gcttcacctt
      481 ctattatgct tataatttca gcatccttaa tctctgtaaa ataatagact ctgcccaccg
      541 atttgtcaaa ataatgcccc gacaaacaca ggttgacacg cgggtcaacc cctacaggca
      601 ggttaatttc atccctaaat aatacaaaac tccacctgcc aatgtaagaa cacctgcttt
      661 aataatgtat ggataaacat ctttcagtgt cgtcttgaaa aaatcggtcg taacaataag
      721 acaaaggtgc atcggtgaaa gcataactcc catgaatccg ccgatgaaac caaggagcag
      781 ataattcata tcaacaaccc cgttcacaac aaggaacgga tatatcacag ggaaagatat
      841 cgaaacgaat ccgatagtta tccctgtcag aactccgcat atcataggga caaggaatac
      901 aaacacagcc ggattggaac ctttgagcgc ttcataaact cctgttgcca cacctgaact
      961 tctcactatc gaatcaaaga acaccattcc cgttacaaga aaaatgacat aaaccgtcct
     1021 ttttttcatt acaaacggtt ttatataaac ggcaagatgt ctctctttaa taataaaagc
     1081 aatgagtgaa atcagaacag cccacggcag atcaagtgaa aaaacgatcg aaagtccaac
     1141 cgcaagaagt atcggccacg aaattacaag cagtttcagc gggttttcag cagttgcgag
     1201 ctctgttatt tcctgttttt tgtgtttaag caggataggc agaccggcag aggccataac
     1261 gactgtcatt gcaccctgat aaataaaaag atcagtggcc ggcactttca tcacagttat
     1321 tatccatgca agtcctgcat aaagcgggaa aaaatattcc cagatgtggc gataccagta
     1381 gtttacagcc attttctctt tctgttcata accgggaagg agcttgtcaa caaaaggggc
     1441 cgaaataaga gaccctccag gcatcggcag aaacccgatg aaagctgcac ttgcagcggc
     1501 ggcaatacgg ttatctcgaa atattttcag aaaaattgcg gacattctgc cgaacatctt
     1561 ccctttttcc atcaactcac caagaagcat aaccgacatg atgatgatga aaagatctgc
     1621 cgatcttctg tttataaaag aattgagcca ttctgaaccg atgtcgggaa gatcgacacc
     1681 tgaaataagt gccatgcaaa ttgctccggc gggaagaaca agctcaagac ggattttctt
     1741 tgcaaggaga ataaccatca gaagaatcgg aattaaaact tgtatccaga tcatttctca
     1801 cctttttaca aacaattaca gcggtataga ataaatttat ccctgatatt cgctgacacg
     1861 accgttttta aggatatatt tcttcccgtc acttttatct gtggctatat cggaactatc
     1921 aaagcagagt ttgtttccgc ttactccgac ccagccgtgc tgacgggcag gaactccgta
     1981 aacaagtgca aaatccggaa catctttggt gacaagtgat cctgctccga tgaatgcata
     2041 tcttccgaca gtggctccgc agactattgt tgcgttggct ccgacagaac accctttttt
     2101 cataagtgtt tttctgaatt cctctttcct ttcgatgaat gatctgggat ttatgacatt
     2161 ggtaaaaacc atcgacggac caagaaaaac atcgtcttct atctcaactc cttcatatac
     2221 tgaaacattg ttctgaacct tgacaccgtt gcccactttc acattcgctc cgacaacaca
     2281 gttctgcccg aaaacacagt tttttccaat agttgtattc ttcagaatgt gtgaaaaaca
     2341 ccagatctta gtcccctcgc ctatcctgac attatcatca attatcgcag tttcatgaac
     2401 aaaaaaattc ttttccatta tcttctccaa cttttaagga acgggtggta gtcgcctctc
     2461 aatccgacag gacaggcatt tctgatgtta aatgctatct caacggccgt tcttgaatct
     2521 tcaagcgaaa aaccttcttt ggcaaggatt ttctgatagc tgacagtgtg aagatcggta
     2581 aatccttcgc tgaattcaag ctcttcacca tcaattgtga tacatctaaa agtctttttc
     2641 cctttttcga tcgcctgctt tggcagcatg ttctcattaa tgctcaggaa ccatcttact
     2701 ctcgcctttt caaactgcat gaaaccggca gcaacatccc gttcctgcac attgacgatg
     2761 tttttctgaa catatccgaa tatccactga agcatatcaa agaaatggat cccgatgtta
     2821 gtcgccattc cccccgattt gctcatgtcg cctttccatg acacatcata ccatctgccg
     2881 cggcttgtga tatatgtcag atcgacatca tacactttgt ctgacggatc ttcatctatc
     2941 ttctttttca atgcggcaat ggtcggatga agccggagct gaaggatcgt attgacatgg
     3001 tggccggtct ctttttcaat tatcttcagg ttgtcaacag tccagggatt aaggacaagc
     3061 ggcttttcac atatcgcatc cgacatgttc ctcaatgcca gacggatatg ggcatcatga
     3121 agatagttcg gtgaggcaat gcttacataa tctattctgt tctctcttct cctgagctta
     3181 tcgatgtgcc tgtcaaacct ttcaaattca acaaagaact ccgcttcagg aaaataggag
     3241 tcgattattc cgatactgtc attgggatca agcattgcaa gaagatcgtt tcccgtctcc
     3301 ttgatcgcct tcatatgtct tggagcaata tatcccgctg cgccgatcag agcaaaattc
     3361 tttttcatca aagtctccct gtaacgatat tcctgtcaag aaacccttta atatcataca
     3421 ttactgtgtt tttatcaaca aatcctttca aatcataact tctgaatttt tcatgcgaaa
     3481 cagcaagaac aaccgcatcg taatcgtttt tcagacattc tgagaaattt gaaagttcga
     3541 ctccgtattc ttccagaact tcatgatgat cagccatcgg atcaacaaca tcaacaatgc
     3601 atccgaattc cctgaattca ctgatgacat caataactct cgagtttctt atatcagggc
     3661 agttctcctt gaatgtgatg ccgagcacga gaactctcga acctttaatc tttttatttt
     3721 tctggatcat cagctttaca actttatttg cgacatatat tcccatgttg tcattaatcc
     3781 ttcttccggc aaggataact tcaggattgt atcctactgc ctgtgctttg tgagtaagat
     3841 agtatgggtc aacgcctatg cagtgtccac cgacaagccc cggcctgaac ggcagaaagt
     3901 tccatttggt gcctgcggct tcgagaacat caatagtgtc tatcccgagt ttttcaaata
     3961 tcagtgcgag ttcatttaca aaagcaatgt ttatatccct ctgagaattt tcaatgactt
     4021 ttgccgcttc tgctactttc acgcttgaag ctttgtgtgt acctgccgtg attatcgttt
     4081 tataaagctg atctatctct tctgcagatt caggggtgga accacttgtt atttttttta
     4141 ttgttgttac tgtgtgaacc ttgtctccag ggttgactct ttcaggggaa aaaccgcaga
     4201 aaaaatcaac attgaactta agtcctgata ttctttcaag tataggaaca caaacctctt
     4261 cagtacagcc tggataaacc gttgattcat atataacaat atcgcctttt ttaagtgctt
     4321 tcgcaactgt ttctgtcccc tttacgacag gtgtaaggtc gggctgtttg tgctcgttca
     4381 ccggtgtcgg gactgtaact atgaaaaaat tgcagttttt tatgtcattt atatcgcttg
     4441 tatagacgag cttctgagct tgtttaagtt ttgccggctc aacttcaagt gtccgctcaa
     4501 ccccttttct tagttcttca agcctttttt tatttatatc gaatccgaca gtatcatatt
     4561 ttcttccgaa ttccacagca aggggaagac ccacataacc aagtccgata atgcaaattt
     4621 tcttctcttt catttgatgc tccttctttc cttacctgta taaactgccg atttctgatc
     4681 tatgttccgc atcggtttct gtgtcttctt ctcttcataa tagtgagcta tcagccccgg
     4741 cactctcgaa atcatgaaaa taccgtttgc aagttctcca ggtatatcca aagcaagaag
     4801 cactgcgccg atcattccgt caacatttac agg
//
LOCUS       DAOOYB010000217         4830 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_20465_length_4830_cov_6.371728, whole genome shotgun sequence.
ACCESSION   DAOOYB010000217 DAOOYB010000000
VERSION     DAOOYB010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4830
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20465_length_4830_cov_6.371728"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            251..535
                     /locus_tag="PL056_10920"
     CDS             251..535
                     /locus_tag="PL056_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47569.1"
                     /translation="MSGRKKIVEEEKTEQKKLMELLEEGIPEISENRKKELAEAAQKT
                     ISRTKPVSIRMQLNDLSAIQQKANEIGVPYQTLINLLIRQYIQGKIQLTL"
     gene            complement(572..1459)
                     /locus_tag="PL056_10925"
     CDS             complement(572..1459)
                     /locus_tag="PL056_10925"
                     /inference="COORDINATES: protein motif:HMM:NF019371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HPM47570.1"
                     /translation="MSEKTCFRKPVLSLEEQVALLISRGMAVKNEENAKSFLRDVNYY
                     RFSGYAIYYEIFQNGQRTHVFKPGTEFEKVKNIYWFDSELRSILFQAIEPIEIAFRTA
                     VCIEASLFYNDSHWYGLNDIFDHRFDKNDFFDLCNKEIQQSHEIFVKKYKEKYSSPEL
                     LPCWMLTEIISLGRWSRIYGSLKERIIRKRVSDRFDCSEKELKSWMHGLTMLRNLCAH
                     HSRVWNRNFSIMISVRDDWMKFVENIDKIAVYFPLLIHLHNTLSDNYGIQNVFDEFLN
                     NYKGEVDLKIMGFKEQGVS"
     gene            complement(1833..1982)
                     /locus_tag="PL056_10930"
     CDS             complement(1833..1982)
                     /locus_tag="PL056_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47571.1"
                     /translation="MKKNENTLSNSSPHHFDKLKVDPFSLRKEGGKVAFTPLFCEERG
                     KLQIL"
     assembly_gap    2048..2147
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2220..2420
                     /locus_tag="PL056_10935"
     CDS             2220..2420
                     /locus_tag="PL056_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014771529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPM47572.1"
                     /translation="MDVLKQFGNRVRELREKVGISQEELAYKAELHRTYISSIERGAQ
                     NISLRNIQKLAKALGVTMKELV"
     gene            2417..3679
                     /locus_tag="PL056_10940"
     CDS             2417..3679
                     /locus_tag="PL056_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009183618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HPM47573.1"
                     /translation="MKAKKSKRQDEVVKYSDYKKNRGFEKLTAENMDEWAKISHAIQS
                     IGHSSEKYYAKYLKNSESEFTKNAKISESALYQPGLFEIDVPFPPPKEPKFTFIDLFA
                     GIGGFRIAMQEVGGKCVYSSEWDQQARLTYEANFGETPYGDITKKETKGFIPDGFDVL
                     CAGFPCQAFSIAGRRGGFDDTRGTLFFDVAEIIKKKQPKAIFLENVKGLRNHDKGKTL
                     ETILNVLRNDLNYFVPDPQIINAKDFGVPQNRERIFIVGFRKDIGVKDFKYPKPLKKE
                     VAFSQVKEKDPVPTKYYISTQYLKTLKNHKSRHESKGNGFGFEIIPDDGIANAIVVGG
                     MGRERNLVVDKRITDFTPTTHIKGEVNKEGIRKMTPREWARLQGYPDSFKIVVADAPA
                     YKQFGNSVAVPAIKATGKNIINLILEEK"
     gene            3676..4305
                     /locus_tag="PL056_10945"
     CDS             3676..4305
                     /locus_tag="PL056_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47574.1"
                     /translation="MTILNQLGITVGKGKKGDTLFDELFPNFLDLKYKTPSEYVSKYW
                     ELFKSHKNYDSNLNGKIFEYILATILIRENFLPLYLSAKVAFVPNVTFDIMVYTAQNG
                     PICFSAKTSLRERYKQADLEAIALKYVHRKSLSYLLTLEENEAKSVKRKIQTGDVIGI
                     NDVIVANCSEFDDLIKDLKKLKLSDPPTVKVIESNQIITADKLKLIKKK"
     gene            4302..4757
                     /gene="vsr"
                     /locus_tag="PL056_10950"
     CDS             4302..4757
                     /gene="vsr"
                     /locus_tag="PL056_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch endonuclease Vsr"
                     /protein_id="HPM47575.1"
                     /translation="MTDHLTPFHRSWNMSRIHSKNTKPEKIVRSMLFSMGFRFRINRK
                     DLPGKPDIVLPRYKTVIFVHGCFWHRHNCRSATMPKSNTDFWEKKFSANVERDQRVKS
                     ELAALGWKVITVWECELKDLEILKLKLISRINDEELMMVAEKKQEYNKQ"
BASE COUNT         1613 a          808 c          933 g         1376 t
ORIGIN      
        1 cactcattgc cccaggccgt tgaccgtggg ctaagttagc gcgacctgtc ttgagcatgt
       61 cgaaaggaac cttcagtccg ctgtcctgac cgcagacttg tccgcaacag ctttatgcga
      121 aggcggaagt tttaacgaag gtggttccac ctttccattg tttcaagaat tagtcgccta
      181 atgatttatg cattgacaaa gtatatacta agaattacaa tcaatggttt tttataaagt
      241 ggaggtcaaa atgagtggca ggaagaaaat cgtggaagag gaaaagacag agcagaaaaa
      301 gttgatggag ctgcttgaag aaggtattcc tgaaatttca gagaacagga aaaaagaact
      361 tgctgaagca gcgcagaaaa ctatttccag aacaaagccg gtgagtattc gtatgcaact
      421 gaatgatctt tcagcaatac agcaaaaagc aaatgaaatt ggtgttccgt atcagacact
      481 cataaatctt ttgataagac agtatattca ggggaaaatt caactaactc tttaaatcaa
      541 gaaccttttc ccaagctttt ttatccgaac ctcaactcac cccttgctct ttaaatccca
      601 taatcttcaa atcaacttct cctttgtaat tattaaggaa ttcatcaaaa acattttgaa
      661 taccgtaatt atcactcaaa gtgttgtgaa gatgtatcaa caatgggaaa taaacagcga
      721 ttttatcaat attttctaca aatttcatcc agtcatctcg cacagagatc attattgaga
      781 aatttctatt ccaaaccctt gaatgatgtg cacacagatt tcttaacatt gtcaaaccat
      841 gcatccatga cttcaattct ttttcagaac agtcaaatct gtcagaaact cttttcctga
      901 taatccgctc ttttaaactg ccgtatattc ttgaccatct tccgagtgag attatttctg
      961 tcaacatcca gcagggaagc agttcggggg atgaatattt ctctttatat ttttttacaa
     1021 atatttcatg gctttgctgt atttctttat tacacagatc aaaaaagtca tttttgtcaa
     1081 accggtggtc aaatatatcg ttcaatccat accagtgaga atcattataa aataaagaag
     1141 cttcgataca taccgctgtt ctaaaagcaa tctctatcgg ttctattgcc tgaaaaagta
     1201 ttgatctaag ctcagaatcg aaccagtaaa tgtttttgac tttttcaaat tcggttcccg
     1261 gtttaaaaac atgagttctt tgtccattct gaaaaatttc atagtaaatg gcataaccgc
     1321 tgaaacggta atagttcaca tccctaagaa aacttttcgc attttcttcg tttttgacgg
     1381 ccattcccct tgagataaga agggcaacct gttcttcaag agaaagcaca ggttttctga
     1441 aacaagtttt ttcactcatt gtctctcgtt atgttttgat agtttttgga taaaaaaaag
     1501 ccccgtcctg gtccgcatta ttctttcgaa caagaggcgt gacgggtact attgagttta
     1561 gtgtagcata tgtttttata aaaagcaacc gctgtaacat ctttttttta ttcgcaaaag
     1621 catctattaa tcaaaggact aatttcgata agcaaagata tcatatatat aacggaaaac
     1681 taatcaaata tttattaatg aaaaaatata ataactaaga attatatatt gtctatcgct
     1741 tctttgcaag aacctttccc gaacttttaa ccaagcccac gggcgaacac accgatcttc
     1801 gtcaagacta cgacaggcaa gcaggttcgc ccctacagga tttgcagctt gcccctctct
     1861 tcacagaaaa gaggggtaaa tgccactttt cctccttctt tccgtagaga gaaggggtca
     1921 accttgagct tgtcgaaatg gtggggggat gagttagaaa gggtgttttc attttttttc
     1981 attattgtcc cgaaatgata gttattgact gtagagttat tgtgtgcagg gttttatact
     2041 tttcaaannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaaa tgatagttat
     2161 tgactgtaga gttattgtgt gcagggtttt atacttttca aaatttccgg aggtggacaa
     2221 tggatgttct taagcagttt ggcaacaggg tcagggagtt aagggaaaag gtcggtattt
     2281 ctcaggagga gcttgcttat aaagctgaac ttcacaggac ttatatcagc agtattgaaa
     2341 gaggagctca gaacatttct ctgaggaata ttcaaaagtt agcaaaagca cttggtgtga
     2401 caatgaagga gttggtatga aagcaaaaaa gagcaaaagg caggatgaag ttgtaaaata
     2461 cagcgactat aaaaaaaata gaggatttga aaaacttaca gccgaaaata tggatgaatg
     2521 ggcaaaaata tcacatgcaa ttcaatctat tgggcacagt tcggaaaaat attatgcaaa
     2581 atatctgaaa aattctgaaa gcgaatttac aaaaaatgca aaaataagtg aatcagcttt
     2641 atatcaacct ggattgtttg aaattgatgt tccattccca ccgccaaaag agccgaagtt
     2701 tacttttatt gatctttttg caggaattgg cggattcaga atagcaatgc aggaagttgg
     2761 tggaaaatgt gtttattcct ctgagtggga tcaacaagca agattaacat atgaagctaa
     2821 ttttggagaa actccttatg gtgacataac aaaaaaggag acaaagggct ttattcctga
     2881 cggttttgat gttctttgtg ccggattccc gtgccaggct ttttcaatag ccgggcgaag
     2941 aggcggattt gatgacacaa gaggaactct tttttttgat gttgcagaaa taataaaaaa
     3001 gaaacagcca aaagcgattt ttcttgaaaa tgtcaaagga ttaagaaatc acgataaggg
     3061 taaaactctt gaaacaattc ttaatgtttt aagaaatgat ttgaattatt tcgttcctga
     3121 tcctcaaata attaatgcaa aggactttgg tgtcccgcaa aacagggaaa gaatttttat
     3181 tgtaggattt agaaaagata taggtgtgaa agattttaag tatcctaaac ctctgaaaaa
     3241 agaagttgcc ttttctcagg ttaaagaaaa agatccagtt cccacaaaat actatatttc
     3301 aacgcagtat cttaaaactc ttaaaaacca taaatccaga cacgagagca agggaaatgg
     3361 attcggtttc gaaataattc ctgatgatgg aattgcaaat gcaattgttg tcggcggaat
     3421 gggtagagag agaaatttag tggttgataa aagaatcaca gactttacgc caacgactca
     3481 tatcaagggc gaagtgaata aagaaggaat acgcaaaatg acaccaagag agtgggctag
     3541 gctgcaagga tatccagata gttttaaaat agtggttgct gatgctccag cctacaaaca
     3601 atttggaaat tccgtggcgg ttcctgcaat aaaggcaaca ggaaaaaata taataaatct
     3661 aattttggag gaaaaatgac aattttaaat caattaggaa taacggttgg aaagggcaaa
     3721 aagggtgata cgctattcga cgagcttttc ccaaattttc tagatttgaa atataagacg
     3781 ccatcagaat atgtttcaaa atactgggag ttatttaaaa gccataaaaa ttatgacagc
     3841 aatttgaatg gcaaaatatt tgaatacatt cttgcaacta ttttaattag agaaaacttc
     3901 ctacctcttt acttaagtgc aaaagtagca tttgttccca acgtgacttt tgacattatg
     3961 gtttatacag cccagaatgg acctatttgc ttcagtgcaa aaactagttt gagagaaaga
     4021 tataaacagg ctgaccttga agcgattgct cttaaatatg tacacagaaa gtccctgagc
     4081 tacttgttaa cattggaaga aaatgaagca aaaagcgtga agagaaagat tcaaactggt
     4141 gatgttattg ggataaatga tgttattgtt gctaattgtt cagaattcga tgatttgata
     4201 aaggatctta aaaaattgaa gctttctgat ccacccacgg ttaaagtaat tgaaagtaat
     4261 caaataatta cagcggataa gcttaaatta attaaaaaaa aatgaccgac cacttaaccc
     4321 cgttccatcg ttcatggaat atgtcccgga ttcattcaaa aaacactaag cctgaaaaga
     4381 ttgtccgttc gatgctgttt tcaatgggtt ttcgtttcag gataaacagg aaggatctgc
     4441 cggggaagcc ggacattgtt ctgccgcgat acaagacggt gatttttgtg cacggttgtt
     4501 tctggcatag acacaactgc aggagtgcaa caatgccgaa atcaaacact gatttctggg
     4561 agaagaagtt ttccgcaaat gttgagagag atcaaagagt taagtctgaa cttgcggctc
     4621 ttggctggaa agttataact gtctgggaat gtgagctgaa agatctggaa attcttaaat
     4681 tgaaacttat ttccaggata aatgacgaag aattgatgat ggtcgctgag aaaaaacagg
     4741 agtataacaa acaatgacaa acataacaaa atcaaacatt tacgcaaaca tccccaactt
     4801 tgccaaagac gaaataactg aaatcctcgc
//
LOCUS       DAOOYB010000218         4830 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_20468_length_4830_cov_3.640000, whole genome shotgun sequence.
ACCESSION   DAOOYB010000218 DAOOYB010000000
VERSION     DAOOYB010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4830
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20468_length_4830_cov_3.640000"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2518)
                     /locus_tag="PL056_10955"
     CDS             complement(<1..2518)
                     /locus_tag="PL056_10955"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HPM47576.1"
                     /translation="MSQHKNFASTILKYRIVIIIAFAVAVFVGIHSWITIPIDAFPDV
                     TNIQVTVITKTEGLSAEDVESQISYVAEQKLRGLPKVNEVRSLSKSGISQVVVIFEEN
                     TDIFWARQIVFEKLSEVKESLPEGVEPELGPISTGLGEIFQYTLESDSKSLTELRTIQ
                     DWLVAPLLKPVKGVNEVNSFGGYVKQYLVTLNPDLLIKHGLSAGEVIEKIEKSNGNGS
                     GGVIVNGWEQKYLRGLGYFKDIKDIGLVVVDSEDGNPLYLSDIAEISISSEFRQGAVS
                     KDGKGEVVAGMVIMLKDENSREVVDRVKETVEKIKTTLPSDVKLTAFYDRTSLIEACV
                     ETVTGALMQGGLLVILILFLFLAEIRTALVVLLSLPVTFFLTFTVMKYLGMSANLMSL
                     GGLAFSVGMVVDASIVIVENIRRHFCGNQGAADRRTLTIKAVQEVASPVAFSAMIISI
                     ILLPLYSLQGIEGKMFIPLASTMLVAIVISLFTALTIVPVLSEIFLKNHQEKEFGFIK
                     KLHSGYLNLLKKSMVKPQSVFVIAGLFVLTALALIPSIGTEFMPPLDEGAIAINVVKL
                     PNASLDGSVKSAETMEKILMKFKEIETVISKTGRAEISEDPMGPEQTDLMIMLKPKKL
                     WSSKTKQELTEEIAKELGKVPGIRLSFSQPIAPRVNELISGIKSDVAVKIFGEDMTVL
                     KTAADKIAAKMNEIKGATDVKVEQISGMEQLDIVIDRERAARFGLNVADINDAIEIAV
                     KGKVATTFFEGEKRFSVAVRYPEIYRNEIEAVKRLLIRTPDGSSVQLEQVAQIKNVEA
                     PNQISRENGMRRVVTECNVRSRDLGGFIKELKEAAGSVEKE"
     gene            complement(2528..3859)
                     /locus_tag="PL056_10960"
     CDS             complement(2528..3859)
                     /locus_tag="PL056_10960"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPM47577.1"
                     /translation="MNKNWLLYLIGFILILSVSINIYFAFNKKTDDQHNHEEVNDFEK
                     EEEHGHGHGNENSQDIPPEELFSKKCEHDILQFECDECRYELGVVKISQDIFDKKLVT
                     LQHASLFPTERAIVLNGLVEFDSKGKFFAFSTVDGVVAKLFVRAGDKVKKGANLVEIF
                     SSQLAEIKGTYLENRAELALTEKTLTRMKELFESKLISEKEFYEAETNYKMAEIRLNS
                     AKSALIGSGFNDSDLQNIEISQNAGKLLIKSPADGIVNSINVFSGEHVDREKELVEIG
                     NTDEVFVIADIYEKELLAVESLFKTGKTPAIVTASAYKDEIFKGMLHSLSLSIDEKTR
                     RPKARILVTNNDGKLKPGQFVSVKLFSPETTETIAVPYQAVMSDEGRDFVFIHYKDDY
                     YLRRNVQTGKQFSKNIEIISGLEKGEKFVVDGAFLCKSDILRSKMGAGCAE"
     gene            complement(3852..>4830)
                     /locus_tag="PL056_10965"
     CDS             complement(3852..>4830)
                     /locus_tag="PL056_10965"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPM47578.1"
                     /translation="VGKKPHWSVSASQSFLGFMKYSGNTKAADSKKNAAKVRLIYEKN
                     EMKISLKEKYNRISFLLEEKKIAEENSKELTDLFNVVNEKVKNGIARNSESTRLFVEK
                     EKAIDAVSAIESEIKSLSLELHSLMNGKIPASFVTEALSYNQKTFSDPFSVKNHYLIL
                     ENTYEKKSAVFQKNNSIYEYIPDFSIGVLYGKEYETATFAISVGLTLPIWNLSNPSRK
                     SSQALITRLNIEEVELSQNLLSERKILINNLNHLEKRINRYAETIIPASKKSAVESKT
                     LYLAGEENLSAVIETFVTAQSTLSEYLSLVTMYQNIVDVIKFRFGEENE"
BASE COUNT         1360 a         1040 c          835 g         1595 t
ORIGIN      
        1 gttctttttc cacgcttccg gcagcttctt tcagctcctt tataaaacca cccagatcgc
       61 gtgatctgac attacattct gtcacaactc tccgcattcc gttttctctg gaaatctggt
      121 ttggagcttc aacattttta atctgggcaa cctgctcaag ctgaacactg cttccgtcag
      181 gcgttcttat taaaagccgt tttacagctt caatctcatt tctgtagatt tcaggatatc
      241 gcactgcaac agaaaatctt ttctcacctt caaaaaatgt ggttgcgact ttgcctttta
      301 cagcaatttc aattgcatca tttatgtccg ccacattcag accgaatcgt gccgcccttt
      361 ctctatctat aacaatatca agctgttcca tgcctgagat ctgttcaact ttgacatcag
      421 tagctccttt tatttcgttc atttttgctg caattttatc tgcggcggtt ttaagaacag
      481 tcatatcttc accgaaaatt ttcactgcga catcactttt aattcctgaa ataagctcat
      541 ttacacgagg agctatcggt tgtgaaaaag acaaccgtat tcccggcact tttccgagtt
      601 cttttgcgat ttcttcagta agctcctgct ttgttttact gctccacagt tttttcggtt
      661 taagcattat catcagatcg gtctgttcag gtcccatcgg atcttcagat atttcagctc
      721 gtccagtctt gcttattact gtttcgatct ctttaaattt catgaggatc ttttccatgg
      781 tttccgcact ttttaccgat ccgtcaagtg aagcgtttgg aagtttcact acatttattg
      841 caattgctcc ttcatcaagc ggaggcatga attctgtgcc tatagaagga ataagcgcaa
      901 gtgcagtaag cacaaaaagt ccggcgatca caaaaacact ttgcggctta accatagact
      961 ttttaagcag attaagataa ccggagtgca gtttttttat aaaaccgaat tctttttcct
     1021 gatggttttt aagaaaaatt tcagaaagaa ccggcactat tgtaagagct gtaaaaagtg
     1081 agatcacaat agcaacgagc atggttgacg ccagcggaat aaacattttt ccctcaattc
     1141 cctgaagcga atagagcggc agaagaatta tcgagatgat cattgctgaa aatgctacag
     1201 gcgatgcgac ttcctgtact gcttttatag taagtgtcct tctgtctgct gcaccctgat
     1261 tcccgcagaa atggcgtctg atgttttcga caattacgat tgaagcatca acaaccatcc
     1321 cgactgaaaa agccaaaccc ccaagactca tcagatttgc cgacattccc agatatttca
     1381 ttacagtgaa tgtgagaaaa aatgtaaccg gcaaagataa caaaacaaca agtgctgtcc
     1441 ggatctcagc cagaaaaaga aacagaatca atatgaccag cagtcctccc tgcattagag
     1501 cgccggtcac tgtttccaca catgcttcaa tgagagaggt gcgatcatag aaagcagtaa
     1561 gtttgacatc acttggcaga gtggttttga ttttttcgac agtttcctta actctgtcaa
     1621 caacttcgcg ggagttttca tctttaagca tgatgaccat gcctgccaca acttctccct
     1681 tcccgtcttt tgaaacagct ccctgtctga attcagaact gatagaaatc tcagcaatat
     1741 cggataaata gagaggattt ccgtcttctg aatcaacaac gacaagtccg atatctttaa
     1801 tatccttgaa ataaccgagt ccccgcagat atttctgttc ccaaccgttt acaataacgc
     1861 cccccgatcc gttcccgttg cttttttcaa ttttttcaat aacttctccg gcggacaagc
     1921 cgtgttttat aagcagatcg ggattaagtg ttacaagata ctgttttaca tatccaccga
     1981 aactgttcac ttcatttaca ccttttacag gcttgagaag tggcgctaca agccagtcct
     2041 gtatagtacg aagctcagtc agagatttgc tatcactttc caaagtgtat tgaaaaattt
     2101 ctccaagacc tgtgcttata ggtccaagtt caggttccac accttccggc agagattctt
     2161 ttacttcgct gagtttttca aaaacgatct gtcttgccca gaagatgtcg gtgttttctt
     2221 caaaaataac aaccacttgc gatattcccg attttgaaag tgaacgaacc tcgttaactt
     2281 taggcagtcc acgaagtttc tgctctgcca cataacttat ctggctttca acatcttcag
     2341 cggacaatcc ttctgtttta gtgattactg tcacctgtat atttgtaaca tcaggaaaag
     2401 catcaatagg aatggtaatc catgagtgaa ttccaacgaa gaccgcaacc gcaaaagcaa
     2461 taatgattac gattctgtat tttagaattg ttgaggcaaa gtttttatgt tgtgacatgc
     2521 cgccccctta ttctgcacac cctgcgccca ttttggatcg caggatgtca cttttacaaa
     2581 gaaaagcacc gtcaacaaca aacttctcac ctttttcaag ccctgagatt atctcaatat
     2641 ttttagaaaa ttgctttcct gtctgcacat tacggcgaag ataatagtca tctttgtaat
     2701 ggataaacac aaaatcacgc ccttcatcag acataactgc ctgatatgga acagcaatgg
     2761 tttcagtagt ttccggtgaa aaaagtttga cagaaacaaa ttgccccggt tttaattttc
     2821 catcattatt tgtcaccaga atacgggctt tcggtcttct cgttttttca tcaatagaaa
     2881 gagaaaggga atgaagcatg cctttaaaaa tttcatcttt atatgcagaa gctgttacga
     2941 tggccggagt tttgcctgtt ttaaataatg attcaacggc aaggagctct ttttcataaa
     3001 tatctgcaat cacaaaaact tcgtcagtat ttccaatttc gaccagttct ttttcgcggt
     3061 caacatgctc acctgaaaag acattaatac tgtttacaat gccgtcagcc ggtgatttta
     3121 ttaaaagttt tccggcattt tggcttattt caatgttttg aagatcagag tcattaaaac
     3181 ctgaaccgat taaagcactt tttgcagaat tcagacggat ttccgccatt ttgtaattag
     3241 tttcagcttc atagaattct ttttcagaaa taagttttga ttcaaaaagt tctttcattc
     3301 tggtgagtgt cttttctgtc agcgcaagtt cagctctgtt ttcaagataa gttcccttaa
     3361 tttctgctaa ttgcgatgag aaaatttcaa caagattcgc accttttttt accttgtctc
     3421 cagccctgac aaaaagtttc gcaacaactc cgtcaacagt cgaaaaagcg aagaattttc
     3481 ctttagaatc aaactcgaca agaccgttaa gcacaatcgc ccgctctgtc ggaaacaaag
     3541 aagcatgctg aagtgttaca agttttttat caaaaatgtc ttgcgagatt ttcacaacac
     3601 caagttcata acggcattca tcacattcaa actgcaaaat gtcatgttca catttttttg
     3661 aaaaaagttc ttcgggcgga atgtcctgac tgttttcatt gccatgacca tgtccgtgtt
     3721 cttcttcttt ttcaaagtca ttcacttctt catgattatg ctgatcatca gtttttttat
     3781 taaaagcgaa gtaaatattt atacttactg ataatatcag gataaaacct atcaaatata
     3841 acaaccagtt cttattcatt ttcttctcca aaacgaaatt taattacatc aacaatattc
     3901 tgatacattg tgacaagtga gagatattca ctcaaagttg actgtgctgt tacaaaagtt
     3961 tcaatcactg cactcagatt ttcttctcct gccagataaa gtgttttgct ttcaacagca
     4021 cttttttttg aggcaggaat tatcgtttcc gcatatcggt tgattctttt ttccaaatga
     4081 tttaaattgt ttatcagaat ttttcgttca gataataaat tctgtgacaa ttcaacttct
     4141 tcaatgttca gccgggttat cagtgcctgg gaggattttc gtgacgggtt tgacaaattc
     4201 cagatcggaa gtgttagccc gacacttatt gcgaaagtag ctgtttcata ttcttttcca
     4261 taaagcactc ctatcgaaaa atcggggata tactcgtaaa ttgaattgtt tttttgaaaa
     4321 acagcactct ttttttcgta agtattttcc agaatcagat agtggttttt tacagaaaac
     4381 ggatctgaaa aagttttttg gttatatgac agggcttctg tcacaaaaga tgcagggatc
     4441 ttgccgttca tcagagagtg taattcaagt gataaggatt tgatttcact ttcaattgca
     4501 gacactgcat caatagcttt ctctttttcc acaaaaagtc gtgtagattc gctatttctt
     4561 gcgattccgt ttttgacttt ttcgtttact acattaaaga gatcagtcag ttctttactg
     4621 ttttcttcag ctattttttt ctcttctaat aaaaaagaga tccggttgta tttttctttt
     4681 aaagatattt tcatctcatt tttttcgtag atcaaacgga cttttgcagc gtttttcttt
     4741 gaatctgcgg cttttgtgtt tcccgagtat ttcataaaac ctaaaaagct ttgagaagca
     4801 gaaacgctcc aatgcggttt cttgccgaca
//
LOCUS       DAOOYB010000219         4817 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_20524_length_4817_cov_8.181856, whole genome shotgun sequence.
ACCESSION   DAOOYB010000219 DAOOYB010000000
VERSION     DAOOYB010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4817
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20524_length_4817_cov_8.181856"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            700..894
                     /locus_tag="PL056_10970"
     CDS             700..894
                     /locus_tag="PL056_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47579.1"
                     /translation="MKTESGRERIDGREKNKKKIEMSLEKPILAWKRLEFEEKFMMVS
                     NVVEKCTKNVYTNVGNKKKE"
     gene            903..2033
                     /locus_tag="PL056_10975"
     CDS             903..2033
                     /locus_tag="PL056_10975"
                     /inference="COORDINATES: protein motif:HMM:NF024555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47580.1"
                     /translation="MKKSIRPPENWQDFESLCKKLFGEVWGCPYTIKKNGRLGQPQAG
                     VDVYGKPKGEENYWAIQCKGKDNYTNSKLTKNEIDEEIEKALTFEPKIKVYALATTAT
                     KDAEIEKYVRIKDIESAKKGGFEIILYSWEDIADLIEEHRDTFNWYVNNLQFKEQFDI
                     EVSFQTENDKNILTPKFQKTITTYTQKPELELQTSKPILGYATRSLVHPITSLDNSIF
                     GPKKKNHGWCTLKVRIFNSGNKVLEDWKFWLSFDEESVKDVDDDFTSNPFAYEVAAKY
                     RTTWAYENEKQILYKPLNNAPLIQKDSKVFECHCIPKFDSTSIKVGWQLLARDFDLEG
                     VAEFEVVPEYEIARKRKYVDNSEEVREETEISDYVTTIEKKD"
     gene            complement(2229..2909)
                     /locus_tag="PL056_10980"
     CDS             complement(2229..2909)
                     /locus_tag="PL056_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47581.1"
                     /translation="MISFIFEIVGNVIANTGDCISVVDRIDGLYNRAWSNLLYIIGVG
                     GAVVGFLIPLTIQFFRDRDLRDVKRNVFRVRGQVDSLDKDTKNMTDRQKTIDKKIVDF
                     DKKISDLFASLDVIKVRTQKISLLYCKFNVNISIYFTDKSSRVSVVDSLIEYINSFLD
                     LDEIDGAILALSFMDVVISEAKSSDVQEILESIAELIVRCSKDDRIKDTSSFSELVNN
                     IKKKKAGD"
     gene            complement(2935..>4817)
                     /locus_tag="PL056_10985"
     CDS             complement(2935..>4817)
                     /locus_tag="PL056_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004757106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HPM47582.1"
                     /translation="LPRLHGFEILMASYTMCHLKLEMLLKETGYKPKNQQRLQVYLTN
                     SLEEPNPYTGTIFASWLSQEAAEANYIKRESPVMVVLGNPPYAVSSTNKNEWIQGLIA
                     DYKKDLNERKINLDDDYIKFVRYGEYFIEKNGEGILAYISNNSFIDGITHRQMRKHLL
                     ETFDKIYILDLHGNSKKKETSPDGSKDENVFDIQQGVSINIFVKNNKKKKNELGKVFH
                     SDIFGKRDYKYKHLVDSNIDGITKKELACPHPGYYFVPKDFDLKEEYDSSIRVADLFI
                     ENNTGIQTKRDKFVYQFSEKELEIVLQDIKSMETELLRKKYDLGNDGRDWSVDWAKKD
                     LNNNSGVVTLVHYHPFDLRETFFTGRSKGFMAYPRSPLMSSMIDENIALLSVRNSRQG
                     NTNNFMVTNTIVDKDGISPFDNCKIFPLYLYPQKDNNAVFDNGREREPNLNMEIVQKI
                     ADGLGLKFVKEKVDTEGTFAPIDLLDYIYAVLHSPSYRDKYKEFLKIDFPRIPYPEDA
                     ETFWKLVSKGGELRQIHLMESPILENHEYYFPVEGNNEVEKPEYKGGRVYINKDQYFE
                     NVPEISWNFYIGGYQPAQKWLKDRKGRVLTFDDIQHYQKITKALLETDRVMKEIDEVG
                     LC"
BASE COUNT         1602 a          927 c          852 g         1436 t
ORIGIN      
        1 tgttttcgca acagtgaagc tcttctaaaa aactgactaa ctcttttttg ctcattcctt
       61 taaaattcat aacaacctcc ttaaaccgtt aaaaaacaaa cctttcaccc ctgaaaaatt
      121 tttttcagac ggaagagatg cctaacggcg agtgttcccc aaaaaagcgt atgaaacgga
      181 acggctgtga gtggaacgga aagcggacaa gtcagcatgc acaggaaatt tcaaaaattt
      241 tgtttcgcga tgaatggaac agaggaaaat tttttaaata gccgggaatg taatgaaggc
      301 ggttccgaga actgacttgc gtgaagtgga atagcaaact tggcaataat cttccgtctg
      361 aaaattgatg ctatacgcag tatcaacgca gccgatggct gccgtgaact gtgaagcgtg
      421 agcggtgggg atgactttgt ggctttattg tcttaacgct ttaatgcttt aacgcttagt
      481 tgcttaatgt attaccctgt aaatatttga aatgcagccc gaattcgata aaatagaaat
      541 tgcgatgcat gggaatttag atagccccct gatctccgga aacggttttc agggggtttt
      601 gttttaattc gctatagcga aaaattcact aaagcgaatt tccgtcagct gcctggaggg
      661 agatcggaga tctgttcatt ccgggaagga ataaccgaag tgaaaacgga gtccggaagg
      721 gagcgtatag acggcagaga gaagaataaa aagaaaattg aaatgtcttt agaaaaacct
      781 atacttgctt ggaagagatt agaatttgaa gagaaattta tgatggtttc aaatgttgtt
      841 gaaaaatgca caaaaaatgt atacacgaat gttggcaaca aaaaaaagga ataatttgta
      901 aaatgaaaaa atctatacga ccgcctgaaa actggcagga ctttgaaagc ttatgcaaaa
      961 agctctttgg agaagtttgg ggatgtccgt acacaattaa aaaaaacggt agacttggac
     1021 agccgcaagc tggggtagat gtctatggaa aaccaaaggg agaagaaaat tactgggcga
     1081 tacaatgcaa aggaaaagat aattatacaa acagcaagct tacaaaaaac gaaatagatg
     1141 aagaaataga aaaggccctg acctttgaac caaaaataaa agtatatgcc ttagcaacta
     1201 ccgccaccaa ggatgccgaa attgaaaagt atgtacgcat aaaggatatc gaaagtgcta
     1261 aaaaaggagg ttttgagatt atcttatatt catgggagga cattgctgat ctaatagagg
     1321 aacacagaga tactttcaac tggtatgtga ataatttaca attcaaagaa caatttgaca
     1381 tagaagtgtc atttcaaact gaaaacgaca aaaatattct aactccaaaa tttcaaaaaa
     1441 ccataacaac ttatactcaa aaacctgaat tagaactaca aacaagcaaa cctatactag
     1501 gctatgcaac aagatcactt gtgcatccaa taacttcgct ggacaacagt atatttggac
     1561 caaagaaaaa aaatcacggt tggtgcacat taaaagtccg catttttaat tcaggcaata
     1621 aggttctgga agattggaag ttctggctga gttttgatga agaaagcgtg aaagatgtcg
     1681 atgatgattt tacaagcaac cctttcgctt atgaagtggc cgcaaaatac cggacaacgt
     1741 gggcttacga aaatgaaaaa caaattttat ataaacctct aaacaacgca cctttaatac
     1801 aaaaggatag caaagttttc gagtgtcact gcattccaaa atttgactca acatcaatta
     1861 aagtgggctg gcaactattg gctcgggatt ttgatttaga aggagttgcc gaatttgaag
     1921 ttgttcctga atatgaaatc gcaagaaaaa gaaaatatgt tgacaattcc gaagaggtaa
     1981 gagaagaaac cgaaatcagc gactatgtca caacaataga gaaaaaagat tgacacctcc
     2041 aacaatatta atcccctgga taaaaacaat catcagaaca aaacaccttg ttgtcttaac
     2101 gctttattgc tttaacgctt agttgtttaa tgtataacct tgtaaatact tgaaatgcag
     2161 accagatttg ataaaataga aatgatagaa gcaaatttta acaagaataa ataacaacaa
     2221 aacagctctt aatccccagc tttcttcttt tttatgttgt ttacaagttc gctgaaagaa
     2281 gatgtgtctt tgatacgatc atctttcgaa catctgacaa tcagctcagc tatgctctct
     2341 aaaatttcct gaacatcaga actttttgct tcggaaatca cgacatccat aaatgacagc
     2401 gccaatattg caccatcaat ctcatcaaga tcaagaaaag aattaatata ctcgatcaat
     2461 gaatcaacaa cagagaccct cgaagacttg tctgtgaaat atatagagat atttacatta
     2521 aatttacaat acagcaagga aatcttttgc gttctcactt taattacatc caaagaggca
     2581 aacagatcag aaattttctt gtcaaaatca actatttttt tatctatagt tttctgccga
     2641 tcagtcatat tctttgtatc cttatctaga gaatcaacct gtcctctaac tcgaaaaaca
     2701 tttctcttga catctcgcaa atcacgatcg cggaaaaact gtatcgtcaa cggaattaaa
     2761 aacccaacaa cagcaccgcc aactccaatt atgtacaaaa gatttgacca agccctatta
     2821 tataagccat caattctgtc caccacacta atacagtctc cagtgttggc aataacattg
     2881 cctacaatct caaaaataaa gctgatcatc tcgcccccat aaaatataaa acaactaaca
     2941 taatcccacc tcatcaatct ccttcatcac cctgtctgtt tcaagcaatg cttttgtgat
     3001 tttctggtaa tgctgaatat cgtcgaatgt gaggactcgg ccttttctgt ctttaagcca
     3061 tttctgagcg ggttgatatc cgccgatgta gaaattccat gatatttcgg gaacattttc
     3121 aaaatactgg tctttgttta tatagactct gccgccttta tattcgggtt tttcaacttc
     3181 attgtttcct tcaaccggaa aataatattc atggttttca aggatgggtg attccatcaa
     3241 atggatctgg cgaagctctc caccttttga aacaagtttc cagaatgttt ctgcatcttc
     3301 cggataagga attctcggaa aatcaatctt caaaaattcc ttgtatttat cacggtatga
     3361 agggctgtga agcactgcat aaatataatc aagaagatca atcggggcga atgttccttc
     3421 tgtatcaact ttctctttaa cgaatttcaa tcctagacca tccgcaatct tctgaactat
     3481 ctccatgttt agattgggct cacgttccct gccattgtca aaaactgcgt tattgtcttt
     3541 ttgagggtaa agataaaggg gaaaaatttt gcagttatca aaaggagaaa taccatcttt
     3601 gtcaacaatt gtgtttgtaa ccataaaatt atttgtgttt ccttgtcgag agttgcgcac
     3661 agagagcagt gcaatgtttt catcaatcat gctcgacatc aaagggcttc tgggataagc
     3721 cataaaacct ttgctcctgc cggtaaaaaa agtttccctt aaatcaaaag gatgatagtg
     3781 aaccagagta actactccac tattgttgtt taaatctttc ttcgcccaat cgacagacca
     3841 gtctctgcca tcatttccca aatcatattt tttccttaaa agctcagtct ccatactttt
     3901 tatatcttgc agcactatct ccaattcctt ttcagaaaat tgatacacga atttatctct
     3961 ttttgtctga atacctgtgt tgttttcaat aaaaagatcg gcaactctta ttgaagaatc
     4021 atactcttct tttaaatcaa aatcttttgg cacaaaataa taaccaggat gcggacacgc
     4081 taactctttc tttgttattc catctatgtt gctatcaacc aagtgtttgt atttgtaatc
     4141 tctttttcca aaaatatcac tgtgaaaaac cttccccaac tcattctttt tctttttgtt
     4201 atttttcaca aaaatattaa tacttacacc ttgttggata tcaaacacat tttcgtcttt
     4261 gcttccgtcg ggtgaggttt ctttcttttt tgaattaccg tgaagatcca gaatataaat
     4321 cttgtcgaat gtttcgagaa gatgttttct catctgacga tgtgtgatgc cgtcaataaa
     4381 actgttgttt gagatataag ccagaattcc ttcgccattc ttttcaatga aatattcgcc
     4441 atagcgaaca aacttgatgt agtcgtcatc aaggttaatt tttctttcat tcagatcttt
     4501 tttgtaatcc gcaataagtc cttgaatcca ttcattttta tttgtactac taacagcata
     4561 cggaggattc ccaagcacca ccatgaccgg tgattctctt ttgatataat ttgcttccgc
     4621 tgcttcctgt gaaagccatg atgcgaaaat cgttccagtg taagggttag gctcctcaag
     4681 cgaattggtt aaatatacct gtaatctctg ctgatttttc ggtttatatc ccgtttcttt
     4741 cagtaacatt tcaagtttca gatgacacat cgtgtaactt gccatgagta tttcaaatcc
     4801 gtgaagccgg ggcagaa
//
LOCUS       DAOOYB010000220         4809 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_20564_length_4809_cov_4.386622, whole genome shotgun sequence.
ACCESSION   DAOOYB010000220 DAOOYB010000000
VERSION     DAOOYB010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4809
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20564_length_4809_cov_4.386622"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..1817
                     /locus_tag="PL056_10990"
     CDS             60..1817
                     /locus_tag="PL056_10990"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HPM47583.1"
                     /translation="MKKVFAVLSAVVLSLLISSCGSSKTADVKTGGSCSNEGEYRCNG
                     DVVQGCALGSWIVVVDCSETGQVCKETEAYGITAATCVDSEGGDSGDSGNSGNTGDSG
                     NTGDSGKTSPCNPNPCTKKSNSNGECTVEGETGYSCGCESGYEWYSIECRDIDECSDN
                     TDNCDENADCANTEGSFTCECREGYDGNGLFCQDIDECDAGTDNCSQNATCTNTGGGH
                     VCKCNDGFSGDGISCEDINECELGTDNCKENTTCENIIGSFVCNCNDYFTGSDNKCTF
                     CNKDSQCGMNCETCSGETPYCHDSGSYESHCAECQDDNDCEGGIKCNEMGFCSTESMV
                     LIPSGSFWMGSPTDEPDRESDETLHFVQLTRSFYIGKYEVTQKEFKDIMGYNPSYFSS
                     CGDNCPVEKAKGMEAINYVNELSKLEGFEQCFDCSGSGTSVTCSLKTKFATPYECKGY
                     RLPTESEWEYAARAGTSTPFYNGDYTTENIDAIGWYNGNSDDKTHSVGQKLPNSLGLY
                     DMSGNVWEWTMDRETTYPSGTEESPVIDPWSMNGDLKDYTPRGGGWYTAAKYCRHAKR
                     NGWAYGNTVVIGFRVVRTK"
     gene            1854..>3366
                     /locus_tag="PL056_10995"
     CDS             1854..>3366
                     /locus_tag="PL056_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015345723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding EGF-like domain-containing
                     protein"
                     /protein_id="HPM47584.1"
                     /translation="MKKLFFVFVSIMVLIFVSGCGGSSGAKTDGTCSNEGEYRCNGDV
                     VQGCALGSWIVVVDCSETEQVCKETEAYGITAATCVDGEGGNGDSDDLNGNDSDVISP
                     CNPNPCTNKSNSSGECKVEGETGYSCGCESGYEWRSIECRDIDECSDNTDNCDANADC
                     KNTAGSFTCECRDGYDGNGVTCSDIDECLLGYDNCSQNAYCENIVGSFTCECNDGYEG
                     DGVNCSDVDECELGTDGCIETADCANTIGSYTCECKEGYDGNGYFCNDIDECDLETDN
                     CSDNATCTNTTGSFTCKCKTGYQGDGVTCGDINECDLETDNCSDHATCSNTTGSFNCA
                     CTQYFSGSGTECTFCNTDSMCGASCAACSETTPVCRDNGNYTSTCVECLVTDECEEGL
                     ECSEAGLCVAPPDVLFVVLDSSALTASETAVLTKLNSYDTIVTKVVNAGNSTFIQGSL
                     APAVIATEENALTKTVVDSLIENGRKVMLLKNSGTVMGGTWKTDGCCSQRKYLQ"
     assembly_gap    3367..3466
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3467..4689
                     /locus_tag="PL056_11000"
     CDS             <3467..4689
                     /locus_tag="PL056_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47585.1"
                     /translation="PAGNIAFIIYNNNDDDTSPALTSDEQIINNKLIGMGWEDKITYI
                     KSSRVKNSDFSGAVLVVSTEYPTIDKNTVNTLINSGQNMMLLKNSGSVMGGTWKTDGC
                     CSQRKYLQVLSNESFFDGYASNLTFETNTSNASYFIASNYPVGWTVIGGNAYNSNGEK
                     TVLYREHTTSGGKGAIFTYYPQNYNNAGWNSFEMLLDWFFDEDIIEGKTVPAGNIAFI
                     IYNNNDDDTSPTLSSAENAVYTQLYNGGYEDKVTFIKSSRLKNSDLTDAAIVISTQYP
                     TIDKNTANTLISSGKKVFMLYNSGAVMGGTWTSDSNSEALKLVVESNTQYLADYAVSS
                     TLSLQSSQSAVYTTVIPVGWTAIGRNNRNTTYKTALYSTNAGGGRATILAYNPYYFTT
                     TGWTLFDKIIDWLE"
BASE COUNT         1511 a          827 c         1168 g         1203 t
ORIGIN      
        1 atcaaagaac aagtctgaga tacaaaaaaa ttataaccaa ttattcgtag gaggttgtca
       61 tgaaaaaagt atttgcagta ttgtcagcag ttgttttatc actgcttatt tccagttgtg
      121 gaagcagcaa aaccgctgat gttaaaactg gcggctcatg ttcaaatgag ggtgaatatc
      181 gttgcaacgg ggatgttgtt cagggatgtg cccttggttc atggatagtt gttgttgatt
      241 gcagtgaaac cggtcaggtt tgcaaggaga cggaagctta cggaattact gcggcgacat
      301 gtgttgacag tgaaggtggt gattcaggtg acagcggcaa ttcaggcaat acaggtgatt
      361 ccggaaatac aggtgattct ggaaaaacaa gtccgtgtaa tcccaatccg tgcactaaaa
      421 aatcaaacag taacggcgaa tgcactgttg aaggtgaaac agggtacagt tgcggctgtg
      481 aatcgggata tgaatggtat tcaatcgagt gcagagacat agacgaatgt tctgacaata
      541 ctgataactg tgatgaaaat gcagactgtg ccaacaccga aggatcgttc acctgtgaat
      601 gcagggaagg atatgacggt aacggacttt tctgtcagga tatagatgaa tgtgatgcag
      661 ggacagacaa ttgcagtcag aatgcgacat gcacaaacac gggaggaggt catgtctgca
      721 aatgcaacga cggattcagc ggagatggaa taagctgtga agatataaat gagtgtgaac
      781 tcggaacaga caactgtaag gagaacacga cttgtgaaaa tatcattgga tcatttgttt
      841 gcaactgcaa tgattacttc accggttctg ataataaatg cacattctgt aacaaggact
      901 ctcaatgcgg gatgaattgt gagacatgtt caggagaaac accgtactgc catgattcgg
      961 gcagttacga atctcattgt gctgaatgtc aggatgacaa tgactgcgaa gggggaatta
     1021 aatgcaatga aatgggattt tgcagtactg aatcaatggt gttgattccg tcaggcagtt
     1081 tctggatggg ttcgccgaca gatgaacctg acagagagag tgatgaaacg cttcatttcg
     1141 tccaattaac cagaagtttt tacataggga aatatgaagt gacacaaaaa gaatttaagg
     1201 atatcatggg ttacaatcca agctattttt caagttgcgg agacaactgc cctgtagaaa
     1261 aagcaaaggg tatggaagct attaattacg ttaatgaatt gtcaaaattg gaaggatttg
     1321 aacagtgttt tgactgctca ggaagtggaa caagtgtgac ctgttcttta aaaacgaagt
     1381 ttgcgacacc ttatgaatgc aaaggatacc ggctcccgac ggaaagcgaa tgggagtatg
     1441 ctgcaagagc tggaaccagc actccttttt ataatggtga ttacacgaca gaaaatatag
     1501 atgctatcgg ctggtataat ggaaactcag acgacaaaac acattctgta ggtcaaaagc
     1561 taccgaattc tttgggactt tatgatatga gcggcaatgt ctgggaatgg acaatggata
     1621 gagaaacgac ttatccaagt ggtacagaag aatcacccgt aatagatcct tggagtatga
     1681 atggagattt aaaagattat actccgaggg gtggcggctg gtatactgca gcaaagtact
     1741 gccgacatgc aaaacgaaat ggctgggcat atggaaatac cgtggttatt ggtttccgtg
     1801 tagtaagaac gaagtaaaaa aggttcgatt aattgtaaaa tatggagtca atcatgaaaa
     1861 agttattttt tgtttttgta tcaattatgg ttctgatttt tgtcagcggt tgcggcggga
     1921 gcagcggagc aaaaacggac gggacatgtt caaatgaggg tgaatatcgt tgcaacgggg
     1981 atgttgttca gggatgtgcc cttggttcat ggatagttgt tgttgattgc agtgaaaccg
     2041 agcaggtttg caaggagacg gaagcttacg gaattactgc ggcgacatgt gttgatggcg
     2101 agggcggtaa cggagacagc gatgatctga acggaaatga ttccgatgtg atcagtccgt
     2161 gcaatcccaa tccgtgcact aataaatcaa acagtagcgg cgaatgcaaa gttgaaggtg
     2221 aaacaggata cagttgtggc tgtgaatcgg gatatgaatg gcgttcaatc gagtgcagag
     2281 acatagacga atgttctgac aatacagaca actgtgatgc aaatgccgac tgtaaaaaca
     2341 cggcaggttc attcacatgc gaatgcaggg acggttatga cggaaacggt gtcacttgca
     2401 gcgatattga cgagtgtctt ctgggttatg acaattgcag tcagaatgca tattgtgaaa
     2461 acattgtcgg aagttttacc tgtgaatgta acgatggtta tgaaggtgac ggtgtcaatt
     2521 gcagtgatgt ggatgagtgt gaactcggaa ctgacggatg tatcgaaacg gcagactgtg
     2581 ccaatacaat cggttcgtac acctgcgagt gtaaagaagg atatgacggc aacggttatt
     2641 tctgcaatga tattgacgaa tgtgaccttg aaaccgacaa ttgcagtgat aatgcgacat
     2701 gcactaatac gacaggaagt ttcacatgca agtgtaaaac cggttatcag ggagatggtg
     2761 ttacctgcgg cgatatcaat gaatgtgatc ttgaaacaga caattgcagc gatcatgcga
     2821 catgttcaaa cacgacaggg tctttcaatt gcgcttgtac tcagtatttt tcaggcagcg
     2881 gaaccgaatg cacattctgc aatacagatt ctatgtgcgg ggcatcatgt gcggcatgtt
     2941 cagaaaccac accggtttgc agagataacg gcaactatac ttcaacatgt gtcgaatgcc
     3001 ttgtgacaga cgagtgtgaa gaaggattgg aatgcagcga agccggatta tgtgtggcac
     3061 cgccggatgt gctgtttgta gttcttgaca gcagcgcact cacagcaagt gaaacggcag
     3121 tactgacaaa actgaacagc tatgacacca tcgtgacaaa agtggtcaac gcaggaaaca
     3181 gcacatttat tcagggaagc ctcgctccag cagtcatagc aacagaagaa aatgcactca
     3241 caaaaacagt agttgattcg ttaatagaaa acggcagaaa agtaatgctg ctgaagaatt
     3301 ctggcacggt catgggaggt acatggaaaa ctgacggctg ttgttcacaa aggaaatatt
     3361 tacaagnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnttcc tgccggtaat
     3481 atcgcattca taatttataa caacaatgat gatgatactt cccccgcact tacatccgat
     3541 gaacagataa tcaacaacaa acttatagga atgggatggg aagacaaaat aacttatatc
     3601 aagtcaagta gagtaaagaa ttctgatttt tccggggcgg tgcttgttgt gtcaacagag
     3661 tatccgacaa tagataaaaa cacggtaaat acattaatta actccggaca gaacatgatg
     3721 ttgctgaaga attctggttc ggttatggga ggtacatgga aaactgacgg ctgttgttca
     3781 caaaggaaat atttacaagt tttatcgaat gaatcttttt ttgatggtta tgcatcgaat
     3841 ttaacctttg aaacaaacac atcaaatgca tcatatttta tagcttcaaa ttatccagtt
     3901 ggctggacag taataggagg aaatgcatac aattcgaatg gagaaaagac tgtgctttac
     3961 cgtgagcaca cgacgagtgg agggaaggga gcaatattta cttattatcc tcaaaattac
     4021 aataatgcgg gctggaacag ttttgagatg ctgctggact ggttttttga tgaggatatt
     4081 attgaaggga agacagttcc tgccggtaat atcgcattca taatttataa caacaatgat
     4141 gatgatactt caccaactct ttccagtgct gaaaatgccg tttacactca actttataat
     4201 ggcggatatg aagacaaggt gacatttatc aaatcaagca gacttaaaaa ctcagacctg
     4261 acagatgccg ctatagttat atccacacag tatccgacaa ttgacaaaaa tacagcaaat
     4321 acccttatca gttcaggaaa aaaagttttc atgctttaca attcaggagc cgttatgggt
     4381 ggaacatgga catctgacag caattctgaa gcactgaagc tggtcgttga atcaaacact
     4441 caatatcttg ccgattatgc ggtatcaagc acactttctc tgcaatcatc tcaatctgcg
     4501 gtctatacca cagtaatccc cgtcggctgg acagcaatcg gcagaaacaa tcgcaacacg
     4561 acatataaaa cagcattgta cagcacaaac gcaggtggtg ggagagctac aatattagct
     4621 tataaccctt actatttcac taccaccggc tggacactct ttgacaaaat tattgactgg
     4681 ctggaatagg gagaggaaag ttaaatgaaa aaaatactga ttatcttctt cttcactctt
     4741 gctgtaattg cctgcagtaa gaaagcggat gacgatattg taagaaaaaa gatcgttgct
     4801 tcagagggt
//
LOCUS       DAOOYB010000221         4795 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_20647_length_4795_cov_11.190928, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000221 DAOOYB010000000
VERSION     DAOOYB010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4795
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20647_length_4795_cov_11.190928"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            109..1059
                     /locus_tag="PL056_11005"
     CDS             109..1059
                     /locus_tag="PL056_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47586.1"
                     /translation="MSRKIIITAFAFILFFANDVYAQKKTSVLKLCAKMDGEPFILFT
                     DDSSKASSLGFKKYKDTNLFMRDFKIMVDNVHSAYFKPVEKTGNCITIFRIEPGKKSV
                     SLDFKSVSYTSIQTKEPATVNFKPDLLHNGNIEIVEGPTATLIINQSADSISAYQAMD
                     NKEIKECSDKCQVPVGIPVYFMIKSQDEKIKCPTKFELIVDSEDERKLDCYDEKIIQK
                     ALADFVEKNNVLCHVNVEYAFFKVYGEGCLFSLEADKEGLRYQTPELKLLPLDKQKFK
                     YFWKINSEEKKPYPFSGDRKGAERTPAEGDVIEIIEERNF"
     gene            1056..1658
                     /gene="purN"
                     /locus_tag="PL056_11010"
     CDS             1056..1658
                     /gene="purN"
                     /locus_tag="PL056_11010"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HPM47587.1"
                     /translation="MKIGVFISGTGTNMEAVCKNFISGNLSGVSEISFVLSDKRSAEG
                     IEKARALGINTIILPKKKDEDRDQYEKRLLEAIAPFSTGLIVLAGFMKVLGHTFLAGY
                     SGRIINIHPSLLPSFKGVDAQSRAFEYGVKVSGCSVHFVDETLDGGPIIIQQPVFREN
                     SDDIDTFKSRILREEHIILSKAITIVAEGKYSISDRFVLL"
     gene            1674..2867
                     /locus_tag="PL056_11015"
     CDS             1674..2867
                     /locus_tag="PL056_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47588.1"
                     /translation="MKLKIFLLTLVLTGIMYLAADYLFLRYTRANFIESAKNSVSQSV
                     SLYQYIHRADSFASIKNVELHAKKPELVAAFDFEKWDAVIQQSPKDFSKLSEESKKIV
                     KNIEQNVQVELNVINKMYDSNDALFVVDRHGNVVAKNLDGIFRNKNLAEELLIKTVLQ
                     GFSDTDVIKILDKTYLVTAAPIEKNGHIIGAYCSADNVNSETAKEASTSLYNESLFKD
                     NSRFYFGFFDKQSLLGSTLPTELHESFKKFIADNQELIKKIDTEGSKHDIMIELKNEQ
                     FFSSISRHPSLSETNDMFYIALVSKDKLLDPVQARHGTFGLISFFMILVSFIFSFIID
                     EQFNKPINKFMENMLEIINGNTKFRFSNDAEGVEGNLNQNANMMIATLLGEKSPDKEK
                     LDPDQ"
     gene            2876..3325
                     /gene="ssb"
                     /locus_tag="PL056_11020"
     CDS             2876..3325
                     /gene="ssb"
                     /locus_tag="PL056_11020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HPM47589.1"
                     /translation="MSSFNSLNKVLLLGRLGRDPEKKYTGSGTAVCNFSVATSESVKK
                     GTGYEEKTEWHKIIVFGNQAENVSKFVKKGSLVFIEGRIQSRSYQDKDGNEKYVTEIV
                     ANTIRFLDIKESADSERVYHNKETKNEQPSQPSENTADIAEEDDLPF"
     gene            complement(3332..4654)
                     /locus_tag="PL056_11025"
     CDS             complement(3332..4654)
                     /locus_tag="PL056_11025"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008697748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HPM47590.1"
                     /translation="MKIGVIGSGYVGLVAGACLADFGHEVICMDIDEKKIEGLKKGLL
                     PIYEPGLDDIVERNHRYKRLDFTTDMEHTVKSCDILFIAVGTPPKDDGSADLQYVLAV
                     AENIGKYIDRYKVVVDKSTVPVGTGRLVKKAILEKITERDITIDFDVVSNPEFLREGK
                     SVYDFTHPDRIVIGCESEKASDMMKQVYRVLYINNTPFVLTNIETAEMIKYASNAFLA
                     VKISFINEMALLSEKVGANVQEIARAMGMDGRISPKFLHAGCGYGGSCFPKDTKAIVD
                     IAKQHSIDFKVIKAAIDANEVQKIKMVEKIVLRMGDVKGKILSVLGLTFKPETDDMRD
                     APSLVIISELVKKGAKIKAFCPQGAKEAKWRLSEYSGSIEYCPDSYEAVADSSGVIIL
                     TEWNQFRGLDLEKMRSLMKENYFFDLRNIYAKDSSIRELFQYSGVGIQ"
BASE COUNT         1530 a          969 c          909 g         1387 t
ORIGIN      
        1 ttataataaa ctttcacttc tcaacaaatt ttatttacaa atatttttta aggcaatata
       61 atgcagacct aattgttttt gatgaaatgt gattcgggga gtttccagtt gagcagaaaa
      121 attattatta ccgcttttgc attcattctt ttttttgcaa acgatgttta tgctcagaaa
      181 aaaacatctg ttcttaaact ttgtgcaaaa atggatgggg aaccgttcat cctttttact
      241 gatgacagct caaaagcatc atcgctgggg tttaagaaat ataaagacac caaccttttc
      301 atgagagatt tcaaaataat ggtggacaat gtccattcgg cttatttcaa accagttgaa
      361 aaaaccggca actgtattac catcttcagg atagaaccgg ggaaaaaatc agtttcactt
      421 gatttcaaat ctgtttcata cacaagcatt cagacaaaag agccggcaac ggttaacttt
      481 aagcccgacc ttctccataa tggaaacatt gagatcgttg aaggacctac ggcgacactc
      541 atcataaatc agtcggcaga cagcatttca gcgtatcagg caatggataa taaagaaatt
      601 aaagagtgtt ctgacaaatg tcaggtcccg gtggggatcc cagtttattt tatgataaaa
      661 tcccaggatg aaaagataaa gtgtcccacc aaattcgagc tgatagttga ttcagaagat
      721 gaaaggaaac tggattgcta cgatgaaaag atcattcaga aagcactcgc cgactttgtg
      781 gaaaagaaca atgtgctctg ccatgtaaat gtcgaatatg ccttttttaa ggtttacggg
      841 gaaggatgtc ttttttcgct tgaggcagac aaagaagggt tgcgttacca gacacctgaa
      901 ttaaaacttc ttccgctcga caaacagaag ttcaaatatt tctggaaaat aaattccgaa
      961 gaaaagaaac cctatccctt cagcggcgac agaaaaggcg cggaaagaac acctgctgag
     1021 ggagatgtta tcgagattat tgaagagagg aacttttgaa gatcggtgtt ttcatttccg
     1081 gaacaggaac aaacatggaa gcagtctgca aaaactttat ttcagggaat ctttccggag
     1141 tttctgagat cagttttgtg ctttcagaca aaagatctgc cgagggtatt gaaaaagccc
     1201 gggcactggg aataaacacc atcatactcc ctaaaaaaaa ggatgaggac agagatcagt
     1261 atgaaaaaag actgcttgag gcaattgccc ctttttcaac aggattgatc gttcttgccg
     1321 gattcatgaa agttctcggg cacacattcc ttgcgggata ttccggaagg ataattaaca
     1381 tacacccgtc tcttcttcct tcatttaaag gtgtcgatgc acaaagccgg gcttttgaat
     1441 acggcgtgaa agtttccggc tgttcggttc attttgttga cgaaaccctt gacggaggac
     1501 ccataataat tcaacaacct gtattccgtg aaaattcaga tgatattgat actttcaaat
     1561 cccgaatact aagagaagag catattatac tttccaaagc gataactatt gtcgctgagg
     1621 gtaaatattc aatttcagac aggttcgtcc tgctgtaatg taaaggagga gtcatgaaac
     1681 taaaaatctt tcttttaaca cttgtactga ccggaattat gtatctggcg gcagactatc
     1741 tttttctgag atacacccgt gcaaatttca ttgagtcagc aaaaaattca gtttcccagt
     1801 cagtatcact ttatcagtat attcatagag cagattcttt tgcatcaatt aagaatgttg
     1861 agctgcatgc aaagaaacct gaacttgtcg cggcgtttga ttttgagaaa tgggacgccg
     1921 taattcagca gagtccgaaa gatttttcga aattaagcga agaatctaaa aagatcgtca
     1981 aaaatatcga acagaatgtg caggttgaac ttaacgtcat aaacaaaatg tatgacagta
     2041 acgatgcact ttttgttgtt gacagacacg gaaatgttgt tgcaaagaac cttgacggaa
     2101 tattcagaaa taaaaacctt gctgaagaac ttcttatcaa aactgtactt caggggtttt
     2161 ctgacacaga tgtgataaag atacttgaca aaacctatct tgtaactgcc gcacccatcg
     2221 aaaagaacgg gcatataatc ggggcatact gcagtgccga caatgttaat tccgaaactg
     2281 caaaagaagc ttcgacaagt ctttacaacg aatctctttt taaagataat tcaagatttt
     2341 atttcggatt ttttgacaaa cagtcacttc ttggaagtac gctgccgaca gaacttcacg
     2401 aaagtttcaa gaaattcatt gccgacaatc aggaacttat aaaaaaaata gacacagagg
     2461 gatctaagca cgacataatg atagaactca aaaatgaaca gttcttttca agcatttcga
     2521 gacatccttc tctttcagaa acaaatgata tgttttacat cgcccttgtg tccaaagaca
     2581 aacttcttga tcctgttcag gccagacacg gcacattcgg tctgatatct ttctttatga
     2641 tccttgtttc atttatattc tcgtttatta ttgatgaaca attcaataag cccatcaaca
     2701 agttcatgga gaatatgctt gaaataatca acggaaatac aaaattcaga ttcagtaatg
     2761 atgctgaagg ggttgaaggc aatttaaacc agaatgcaaa tatgatgatc gcgactcttc
     2821 tgggggaaaa atctccggat aaagagaagc ttgatccaga ccaataggag gcactatgag
     2881 ctcttttaac agtttaaata aagttcttct tcttggaaga ctcggaaggg atcctgaaaa
     2941 aaaatatacc ggatcaggaa ctgcggtgtg taatttctcg gttgcgacat cagaaagcgt
     3001 caagaaaggg accggttatg aagaaaaaac cgaatggcac aaaataatcg ttttcgggaa
     3061 tcaagccgaa aatgtttcca agttcgttaa aaagggatct cttgttttta ttgaagggag
     3121 aattcagagc cgttcatatc aggacaaaga cggcaatgaa aaatatgtga ccgaaatagt
     3181 tgcaaataca atacggtttc tcgacataaa agaaagtgcc gactccgaaa gagtgtatca
     3241 caacaaagaa accaaaaatg aacagccttc acaaccatca gaaaatactg ccgacatagc
     3301 tgaagaggac gatctcccct tttaaaaatc cctattgaat gccgacacct gaatattgga
     3361 aaagttccct gatagagctg tcttttgcat aaatgttcct cagatcgaaa aagtagttct
     3421 ctttcatcaa agatctcatc ttttcaagat caagccccct gaactggttc cactctgtga
     3481 gaatgatcac tccgcttgaa tcagctacag cttcataact gtctggacaa tattcaatgc
     3541 tgccgctgta ttcagaaaga cgccactttg cctctttcgc tccctgaggg cagaacgctt
     3601 ttatctttgc accctttttt acgagttctg aaattattac aagactgggg gcatccctca
     3661 tatcgtcagt ttctggtttg aaagtaagac cgagtaccga caatatcttt cctttaacat
     3721 ctcccattct taaaactatt ttttcaacca tttttatttt ctggacttcg tttgcatcaa
     3781 ttgcggcttt gataacctta aaatctatgc tgtgctgttt tgcaatatca acgatggctt
     3841 ttgtatcctt gggaaaacag cttcctccgt aaccgcatcc tgcatgaagg aacttcggac
     3901 tgatgcgacc gtccattccc atggctctcg ctatttcctg aacattagct ccaacttttt
     3961 cagaaagaag tgccatttca tttatgaaag atattttaac agcaagaaag gcgttgcttg
     4021 catattttat catttcagca gtttcaatat ttgttaatac aaacggcgtg ttgttaatat
     4081 atagaactct gtagacctgc ttcatcatat cacttgcttt ttcagattca cagccaatta
     4141 cgatcctgtc gggatgggta aaatcatata ccgacttgcc ttctcttaaa aattcggggt
     4201 ttgaaacgac atcgaaatct attgtaatat ctctttcagt gatcttttcc aggatagctt
     4261 tttttacaag acggcccgtg cctaccggaa cagtcgattt gtcaacaaca actttatatc
     4321 tgtcaatgta ttttccaatg ttttctgcaa ccgccagaac atactggaga tctgcactcc
     4381 cgtcatcttt cgggggagtc cctacggcaa taaaaagaat atcgcagctt tttaccgtgt
     4441 gttccatatc tgttgtaaaa tcaagccgtt tgtatctatg gtttctttca actatatcat
     4501 caagccccgg ctcatagatc ggcaacaacc cttttttaag tccttcgatc ttcttttcat
     4561 caatatccat acagatgact tcatgtccga aatctgcaag acacgcaccc gcaacaagtc
     4621 ctacatatcc ggaaccgata actccgatct tcatttcata cgctccctgt tcagtaaaaa
     4681 aactgtcaga atattatcaa tttgcatgga taactcaaga aaagagaacc atgctttatg
     4741 ctttgaagtt cccccaaaaa aaattcagca gatttctaac tgaagaggag gagct
//
LOCUS       DAOOYB010000222         4792 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_20665_length_4792_cov_14.029132, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000222 DAOOYB010000000
VERSION     DAOOYB010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4792
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20665_length_4792_cov_14.029132"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..1223
                     /locus_tag="PL056_11030"
     CDS             33..1223
                     /locus_tag="PL056_11030"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HPM47591.1"
                     /translation="MRFNRFLEANLLDAVTGFSASNLKADLFLSQFLRSKKIGKRDRL
                     WISDRFYYYLRHKLFFDKISSLNTLPKNIAMMFENEPDDELLKKREELKKTGDFEDLY
                     NRSFSSFLKKRIAGYYKENVFEWLNTKSKIAVRANLHKIQRDILLEKLRENGFEVELT
                     PFSSAGILILNGSSKLKKSELFKEGFFEFQDESSQIASMLVNRNSKTMFDCCAGGGGK
                     SLAVSSLFPEIDVTASDIRFHLFKEIQERAQRAGTKIRTVSHDSVKNRLFDTVFVDAP
                     CSGSGVLRRNPADRYEITERSVENNVALQHELIAKFSENVSNGGELIYVTCSFLKEEN
                     ESIVEKFLNENSGFVLMPACDRLSENGLRNLSEKQITDNGYFRTAPFFERDLMFGAVL
                     KKIR"
     gene            1303..2175
                     /locus_tag="PL056_11035"
     CDS             1303..2175
                     /locus_tag="PL056_11035"
                     /inference="COORDINATES: protein motif:HMM:NF025931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HPM47592.1"
                     /translation="MKKFLILSAIAQFVLSSCSSGPDFSSFDDKVFVECIKKTEKKFE
                     EITELDCSNTEKTLADNPFGETIIKSIKGVEKLVNLKKFAMSKNKIEDISGLSGLKSL
                     EHLDLSENGISDMYPLSGLVNLQYLNLDRNYIGDMRPVKNFENLVELDMSRNGIQGLA
                     EFEKLKKLKKLSLNNNRISEVKSIKDLTELEYLSLNDNAVIEIFDLEPLKKLKVLSLK
                     INRIDVIIVLGKMHSLEELYLADNQIRDLAPIKDHPNLKILDVEMNSIISFDNVAELE
                     SRGCKILGKKIQTK"
     gene            2347..3651
                     /locus_tag="PL056_11040"
     CDS             2347..3651
                     /locus_tag="PL056_11040"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HPM47593.1"
                     /translation="MESFPTIELVGVVVCIIMSAYFSGTETALTALSEIKTSVMGERY
                     PFIQPVFKRWAENPGIILSSILIGNNIVNIFGTLLGGNITYKLMSDYNPAIADLTAGT
                     VMTVLILIFGEVTPKTFAKVNPDKVIVPALFIFRMIEWILLPFSYILSNFAHAVVRIM
                     GGQKGQSGLTQTDIEYLIEKGNDQGVFEQEEQGELLSSVIEFKHTLVKEIMTPRTDTN
                     FLETDTTIKEALDKVEEWGHSRIPVFEDSLDNIRGILYVKDMVTLITKEKTVLSDTIN
                     TIIRTGVFYVPETQKINETLKKMQEDGTHMAIVVDEFGGTAGIITLEDIIEELVGDIR
                     DEDDHDEDQIVMLKDNVLLVDAHISVSDLEEELEISIPDDGEYNSLGGFIVNEVGNVP
                     PEGYILKYNGYEFKVVESNERHIVKVEIRILKDEDETDLPVD"
     gene            3664..4356
                     /locus_tag="PL056_11045"
     CDS             3664..4356
                     /locus_tag="PL056_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HPM47594.1"
                     /translation="MRNKIDLIKNVYTAIKESAENSGRDVTQVRLVAVSKTKPVEDIL
                     QFRRAGIREFGENYVQEFIEKYEALADHDILWHFIGTLQSNKVKYIIDKVYMIHTVDS
                     VSLAAEIQKQAEKKNVHKVKVLIQVNVGNEPQKGGVNPEDLVEIYNEINKFDRISIRG
                     LMSIPPFLEAEELRPFHRKLFDLRDMILKESSADPSEFRELSMGMSADFDVAIEEGAT
                     IVRIGSILFGER"
     gene            complement(4362..4778)
                     /locus_tag="PL056_11050"
     CDS             complement(4362..4778)
                     /locus_tag="PL056_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HPM47595.1"
                     /translation="MKSHRKELWFHTAKRREYLNITHEIQEEVDKSGILEGLCLVNAM
                     HITASVFINDDESGLHRDFEVWLEKLAPEKPHSQYNHNGYEDNADAHLKRQLMGREVV
                     VAVTNGKLDLGPWEQIFYGEYDGMRKKRVLVKIIGE"
BASE COUNT         1545 a          843 c         1044 g         1360 t
ORIGIN      
        1 aatgcactta tacttaagct tgcaggtctg aaatgagatt caaccgtttt cttgaggcaa
       61 atctgcttga tgcagtaacg ggtttttcag catcaaacct gaaagcggac ttgtttttaa
      121 gccagtttct gaggtcaaaa aagatcggca aaagagacag gttgtggata agtgaccgtt
      181 tttattatta tttaaggcac aagctttttt ttgataaaat atcttcacta aatacacttc
      241 caaaaaatat tgcaatgatg tttgaaaatg agccggatga tgaactgctt aaaaagagag
      301 aggaactaaa gaaaaccggt gattttgaag atctttataa cagaagtttt tcgtcttttc
      361 tcaaaaaaag aattgccggg tattataaag agaatgtttt tgaatggctc aatacaaaat
      421 cgaagatagc ggtaagggca aatctccata aaatccaaag agatattctt ttagaaaagt
      481 taagggaaaa tggttttgaa gttgagttga cacccttttc atctgccggg atcctgattt
      541 tgaacggttc atcaaaatta aaaaagtctg agcttttcaa agaagggttt tttgaatttc
      601 aggatgaatc aagtcagatt gcttcaatgc ttgtgaacag aaattcaaag acaatgttcg
      661 actgctgtgc ggggggtggc ggtaaatctc tggcggtaag ctcccttttc cctgaaattg
      721 atgtaactgc aagtgatatc aggttccacc tgttcaagga gatacaggaa agggcgcaaa
      781 gagcgggaac aaagatccgg acagtaagtc atgattcagt aaaaaaccgt ctttttgata
      841 ctgtctttgt tgatgcccca tgcagcggaa gcggagtttt gaggagaaat cctgccgaca
      901 gatatgaaat aacggaaaga tctgtggaaa acaatgttgc gttgcagcat gaacttattg
      961 caaagttttc agaaaatgtt tctaacggag gcgaactgat atatgtgaca tgcagtttcc
     1021 tgaaagagga gaacgaatcc attgttgaaa aattccttaa tgaaaacagt ggttttgtcc
     1081 tgatgccggc ctgtgataga ttaagtgaga acggattaag gaacctgtct gagaaacaga
     1141 taacggacaa cggatatttc aggacggctc cgttttttga aagagacctt atgttcggag
     1201 cggttttgaa aaaaataaga tgatcttttc ttgcacaaat atgagaaatc taatattttc
     1261 ttttcggttc ttttgataaa aacttttaat aacgaggtga aaatgaaaaa gttcctgatc
     1321 ttaagtgcga tcgcacagtt tgttctttcg tcatgttcaa gcggccctga tttcagcagt
     1381 tttgatgaca aggtatttgt tgaatgtata aagaaaacgg aaaagaaatt tgaggagata
     1441 accgaacttg actgttcaaa cactgaaaaa actcttgctg acaacccttt cggagaaaca
     1501 ataattaaaa gcatcaaggg tgtggaaaaa ctggtaaatc tcaagaaatt tgccatgagc
     1561 aagaataaaa ttgaagacat ttcgggtctt tcagggctga aatcgcttga acatcttgat
     1621 ctttcagaga acggaataag cgacatgtac cctctttccg gccttgttaa tcttcaatac
     1681 ctcaatcttg acaggaacta tatcggtgac atgagacctg tgaagaactt tgagaatctt
     1741 gttgaactcg atatgagcag aaacggtatt cagggacttg ccgaatttga aaagcttaaa
     1801 aaactgaaga aactttcact taacaacaac agaataagtg aagtcaaatc aattaaagat
     1861 ctcacagaac ttgaatatct ttcattgaac gacaacgctg tcatcgaaat atttgacctt
     1921 gagccactta aaaaacttaa agttctgtcc ctgaaaataa acagaataga tgtcataatc
     1981 gttcttggaa aaatgcattc tcttgaagag ctttacctcg ctgacaacca gatccgtgac
     2041 cttgctccga tcaaggacca tccgaatctg aagatccttg atgttgaaat gaattcgata
     2101 ataagttttg acaatgttgc agaacttgaa tcccgcggct gtaagatcct gggtaaaaaa
     2161 atccagacga aataaagaac tttcgcaaaa cctttttata ttcagcaatc gggcgttgtg
     2221 ttttaagtgg ttgaaattaa tgacaaatcc tttttgaaag ctttaaaatt caataattag
     2281 ttgtaatatt agccataaca tgtaaaaata gcaggtgttt ttagtaactt gtgaggagga
     2341 attattttgg aatcgttccc tacgatcgag ttggtggggg ttgttgtctg tataatcatg
     2401 agtgcctatt tcagcggcac ggagactgca cttacagcac tttcagagat caaaacttca
     2461 gtgatgggag aaaggtatcc tttcatccag ccggtattta aaagatgggc ggaaaaccct
     2521 gggattatcc tttcaagtat tttgatagga aacaatatcg ttaatatttt cggcacattg
     2581 cttggtggaa acattacata caagctgatg tcggattaca atcctgcaat agctgatctt
     2641 actgcgggaa ctgtcatgac ggtacttatt ctgatatttg gagaggtcac accgaaaact
     2701 tttgcaaagg tgaaccctga caaagtcatt gttcctgcac tttttatatt tagaatgata
     2761 gaatggatac tgcttccttt ttcatatatt ctttcaaact tcgctcacgc agttgtgagg
     2821 ataatgggag ggcagaaagg acagagcggt cttacccaga ccgatattga ataccttatt
     2881 gaaaagggga atgatcaagg cgtttttgag caggaggaac agggggaact gctttcaagc
     2941 gttattgaat tcaaacacac acttgtaaaa gaaataatga ccccaaggac cgacaccaat
     3001 tttcttgaaa ctgatacaac aataaaggaa gcccttgata aagttgaaga atggggacac
     3061 agcagaattc ctgtttttga agacagtctt gataatatca gaggaattct atatgttaag
     3121 gatatggtga ctctcatcac aaaagagaag acagttctat ccgacactat caacacgatc
     3181 ataagaacag gcgttttcta tgttcctgaa acacagaaaa taaatgaaac actcaaaaaa
     3241 atgcaggaag acgggaccca tatggccata gttgtcgatg aattcggcgg tactgcaggg
     3301 ataataaccc ttgaagatat cattgaggaa cttgtcggtg atatcaggga cgaagatgat
     3361 cacgatgaag atcagatagt catgcttaaa gacaatgtgc tccttgtcga tgcccatatt
     3421 tcggtcagcg atcttgaaga agagcttgaa atatcaattc cggatgacgg cgaatacaac
     3481 agtctgggcg gatttatcgt caatgaagtc gggaatgtac cacccgaagg atacattctg
     3541 aaatacaacg gatatgaatt caaagtcgtt gagagcaacg aaagacacat tgtcaaagtt
     3601 gagatcagaa ttctgaaaga tgaagatgaa acagaccttc ctgttgatta gatagaggcg
     3661 cttttgcgga ataagatcga tctcataaaa aatgtttata cagcaataaa ggaatcagcg
     3721 gaaaattcag gaagagatgt gacgcaggtt cggctcgtcg cggtttcaaa gaccaagccg
     3781 gttgaagata ttctgcaatt ccgaagggct ggaataaggg agttcggtga aaactatgtg
     3841 caggaattca ttgagaaata tgaagccctt gcagaccacg acatcctctg gcatttcatt
     3901 ggcacgcttc aaagcaacaa ggtcaaatac attattgaca aggtttatat gatccatacc
     3961 gttgattctg tttcgcttgc cgctgaaatt cagaaacagg ccgaaaaaaa gaatgttcac
     4021 aaagtgaaag tgcttataca ggtcaatgtc gggaacgagc cgcagaaagg gggcgtaaat
     4081 cctgaggatt tggttgaaat atataatgag atcaataagt ttgatagaat ttcaattcga
     4141 ggattgatga gtattcctcc gtttcttgaa gcagaggaac taagaccttt tcacagaaag
     4201 ctttttgatc tgagagacat gatattaaaa gagagttccg ccgatccttc agaattcagg
     4261 gaactttcaa tgggaatgtc agccgatttt gatgttgcaa ttgaggaagg ggcgacaata
     4321 gtcaggatcg gttcgatcct tttcggtgaa aggtagaatg atcattcccc gataatttta
     4381 actaaaaccc gtttttttct cattccgtca tattcaccgt agaagatctg ctcccacgga
     4441 ccgagatcaa gtttcccgtt tgtgacggca acaacaactt ctcttcccat gagctgtctt
     4501 ttgaggtggg catcggcatt gtcttcatat ccgttgtgat tatactgcga atggggtttt
     4561 tcgggggcga gcttttcaag ccatacttca aaatcgcggt gaagacccga ttcgtcatcg
     4621 tttatgaata ctgatgcggt tatgtgcatc gcatttacca gacacaaccc ttcaagaatt
     4681 ccgcttttat ccacctcttc ctgaatttca tgagtaatat taagatattc tcttcttttt
     4741 gcagtgtgaa accaaagttc ctttcggtga cttttcataa tgatctccat tt
//
LOCUS       DAOOYB010000223         4766 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_20818_length_4766_cov_8.431119, whole genome shotgun sequence.
ACCESSION   DAOOYB010000223 DAOOYB010000000
VERSION     DAOOYB010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4766
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20818_length_4766_cov_8.431119"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /gene="pgk"
                     /locus_tag="PL056_11055"
     CDS             <1..388
                     /gene="pgk"
                     /locus_tag="PL056_11055"
                     /inference="COORDINATES: protein motif:HMM:NF012389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HPM47596.1"
                     /translation="PVDHIVAETVNSTETAVYSNGSVPEGFSAFDIGPETLALFKEKL
                     KGCNHLFWNGPVGVYEKKQFAKGSIDLAKAIAKLKTHTVAGGGETALLIKKAGLNEKF
                     SYVSTGGGASLDLLRNGTLPGLDVLK"
     gene            410..2026
                     /locus_tag="PL056_11060"
     CDS             410..2026
                     /locus_tag="PL056_11060"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HPM47597.1"
                     /translation="MNKKTKYIFITGGVVSSLGKGIASTTIGALLESRGIKVNLLKFD
                     PYINLDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFCDVTLKRDNNFTTGQIYDAVL
                     SKERRGEYLGKTVQVIPHITNEIKSKITNLGKTADIVLVEIGGTVGDIESLPFLEAIR
                     QYAYDVGRENVLYIHLTLVPHIKAAGEFKTKPTQHSVKELRMIGIQPDILLCRCEKEL
                     TEDMREKISLFCNVKKEAVFSATDVDSIYELPLLYSRQGVDDIIAKYLNIWSRSAGLQ
                     KWEKINSNIKNPEGEVTIAIVGKYVDLKESYKSLNEALIHAGIANRRTTNLCYIDSEK
                     LEKGELDVDCLKQADGILVPGGFGIRGIEGKIKAVEYARTNKIPFFGICLGMQIATIE
                     FARHVCKMEDAHSQEFNEKSNYPVIDLMEEQKSIKNLGGTMRLGAYTCLVEKGSNAFE
                     AYKSAEISERHRHRYEFNSKFEEELKACGLKITGRDKVTGLVEMVEIEDHPWFLGCQF
                     HPEFKSKPFAPHPLFMAFIKASILNRNKGV"
     gene            2028..2348
                     /locus_tag="PL056_11065"
     CDS             2028..2348
                     /locus_tag="PL056_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47598.1"
                     /translation="MSGLSLESNLDSIPQEPEKEKKESGKPREWKPTVRMQKREQKTR
                     TKLRIRDLYPDKQYIKEVNGKELRYFLLLIIPVIITAIFTAIQREDLYNRLVEILVKV
                     LGKS"
     gene            complement(2350..4311)
                     /gene="uvrB"
                     /locus_tag="PL056_11070"
     CDS             complement(2350..4311)
                     /gene="uvrB"
                     /locus_tag="PL056_11070"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009460444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HPM47599.1"
                     /translation="MEFKLNSAFKPAGDQPQAIEKLIERFKNGSKNELILGATGTGKT
                     FVMANVIRALNRPTLVIAHNKTLAGQLYGEFKNFFPENNVGYFISFYDYYQPEAYIPT
                     TDTYIEKDASRNDDIDRMRHFATASLLENEQCVIVASVSSIYGIGSPDAYRGLTLKLF
                     TGNTFPLKDLCAGLVEIQYERSQFDLNRGKFRVKGDVVEIFPPYEEEIAIRISYFGDE
                     IEEIALIKPFEGKKIRNLDSVRVYPSSHYVAEKETLKKSVELIRTELEERVKYFEKNN
                     KLIEAQRIYERVTQDVAMLETAGYCSGIENYSRYITNREVGDAPPTLLDYFGDDFLVI
                     IDESHATIPQIRGMYRGDRARKDVLVNYGFRLPSALDNRPLNFEEFESRLDRVLYVSA
                     TPREYEISHVSEVIELINRPTGLLDPMPQILPSKNEIDTLYDEVVKEISEGGKVLVTS
                     LTKKMAEDLTDFLKERGIRAKYLHSDIDSIERLRIVMDLRKNLFDVLVGVNLLREGLD
                     IPEVSLVAILDADKEGFLRSESALIQTMGRAARNLKGRVILFADKMTDSIKNAVHETM
                     RRRKIQQEFNELHDITPKSITNKTILDIESHIPGKSKDGIKKAPKHKMLKIIAELENE
                     MKRAATEWDFEYAAILRDKIFQYKAAMEK"
     gene            complement(4393..>4766)
                     /locus_tag="PL056_11075"
     CDS             complement(4393..>4766)
                     /locus_tag="PL056_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47600.1"
                     /translation="SSKKFRNAGIGLLMMSIPLTALAVYGFYDSYNIYLTKEKSKQPE
                     EQSTSGVPFENIGLVLTHFLQLSSFTPAILSITGGSILLYKSLKYKQLNTEPSILTLS
                     SIAPIIDPVSKTYGIALGFSF"
BASE COUNT         1403 a          965 c         1038 g         1360 t
ORIGIN      
        1 tcctgtcgat cacatagttg ctgaaacggt caattctaca gagacggcag tttacagtaa
       61 cggaagtgtt cccgaaggtt tttccgcttt tgatatcgga cctgaaacac ttgcgctgtt
      121 caaggaaaaa ctgaaaggat gcaaccatct tttctggaac gggcctgtcg gtgtctatga
      181 aaagaaacag tttgctaaag ggagcataga ccttgcaaaa gccattgcaa agttgaagac
      241 ccatactgtt gcaggaggcg gtgaaacagc gttgcttatt aaaaaagcag gtttaaatga
      301 aaaattcagc tatgtctcaa caggcggcgg agcttcactc gatcttttaa gaaacggaac
      361 attgccggga cttgatgttt taaagtgatt ttttgataca ggtgttaaaa tgaataagaa
      421 aacaaagtac atttttatta caggcggtgt tgtttcctct cttggcaaag ggattgcctc
      481 aacaacgata ggtgcccttc ttgaaagcag ggggataaag gtcaatctgc tcaagttcga
      541 cccttacatc aatcttgatc ccggtacaat gagccctttc cagcacggtg aggtctttgt
      601 gacagatgac ggtgctgaga ccgatcttga ccttggacat tatgaaaggt tctgcgatgt
      661 tactcttaaa agggataaca atttcacaac cggtcagata tatgatgccg ttctgagcaa
      721 ggagagaaga ggagaatatc tcggcaagac tgttcaggta attcctcaca tcactaatga
      781 gataaagagc aagatcacca atctcggaaa gaccgctgac attgttcttg ttgagatcgg
      841 cggtactgtc ggagacattg aaagtcttcc gttccttgaa gctataaggc agtatgcata
      901 cgatgtgggc agagaaaatg tgctttacat tcatcttaca cttgttccgc acattaaggc
      961 ggcgggcgaa tttaagacaa agccgaccca gcacagtgtc aaggaactgc ggatgatagg
     1021 tatccagccc gatatactgc tctgccgctg tgaaaaggag cttactgaag atatgcgaga
     1081 aaaaatatca cttttctgca atgttaagaa agaagcggta ttttcagcga ccgatgttga
     1141 cagcatatat gaacttccgc ttctttacag cagacaggga gttgatgaca taattgcaaa
     1201 atatctcaac atctggtcaa ggagtgcagg gttgcagaag tgggagaaga tcaattcgaa
     1261 cataaagaac cctgaaggtg aagtgactat cgcaatagtt ggaaaatatg tcgatctgaa
     1321 ggagtcttac aagagtctta acgaagcact tatccatgca gggatcgcaa acagaaggac
     1381 aacgaattta tgttatattg acagcgaaaa gctcgaaaag ggtgaactcg atgttgactg
     1441 tctcaagcag gcggatggaa ttctcgttcc tggcggtttc ggcataaggg gtatcgaagg
     1501 gaagataaaa gcggtggaat atgccagaac aaataaaatt ccgtttttcg gaatatgtct
     1561 gggtatgcag atagcaacga tcgaatttgc aaggcatgtc tgtaaaatgg aagatgcaca
     1621 ctcacaggaa ttcaatgaaa aatctaatta tccagtcatt gacctgatgg aagagcagaa
     1681 aagcataaag aacctcggcg gaactatgcg tcttggtgca tacacatgtc ttgttgagaa
     1741 ggggagcaat gcttttgagg catataagag cgcagaaatc agcgaaaggc accgtcacag
     1801 atacgaattc aattcaaaat ttgaagaaga acttaaagcg tgcgggctta agataacagg
     1861 tcgtgacaaa gtgacaggac ttgttgaaat ggttgagata gaggatcatc cgtggttttt
     1921 agggtgtcag ttccatcctg aattcaaatc aaaaccgttt gcaccccatc cgcttttcat
     1981 ggcattcata aaagcttcaa tattaaacag aaataaaggt gtttaagatg tcgggattat
     2041 ctcttgaaag caatcttgat tcaataccgc aggaacctga aaaggaaaag aaggagtcgg
     2101 gcaaaccgcg tgagtggaaa ccgacagtca ggatgcagaa aagggaacag aaaacaagga
     2161 ccaaactaag aattagagac ctctatcccg acaaacagta catcaaggaa gtcaacggaa
     2221 aagaactcag atattttctc cttcttatca tccctgtaat cataacggca atattcacag
     2281 ccatccagag agaggacctc tacaacagac ttgtagaaat acttgtaaag gttcttggaa
     2341 agtcctgaac tatttttcca tcgcggcttt atactggaat atcttatctc tgagtatcgc
     2401 ggcatattca aaatcccatt ctgtcgcggc ccgtttcatt tcattttcga gttccgcgat
     2461 gattttgagc attttgtgct taggtgcttt tttgatgccg tcttttgatt ttccggggat
     2521 gtgggattcg atgtcaagga tggtcttatt tgttatgctt ttcggtgtaa tgtcgtgtaa
     2581 ctcgttgaat tcctgctgta ttttgcgacg acgcatagtt tcatgaactg cgtttttaat
     2641 tgaatcggtc attttgtcgg caaaaaggat cactcgtcct ttcaaatttc ttgccgctct
     2701 tcccatcgtc tgaatgagag ctgactcact tcttaaaaat ccttccttat cggcatcaag
     2761 gatcgcaaca agcgaaactt cgggaatgtc aagcccttca cgcagaaggt ttactccgac
     2821 aagaacatca aacaggtttt ttctcagatc cattacgatc ctgagccgtt cgatgctgtc
     2881 aatatcgctg tggagatatt ttgccctgat tcctctttct ttgagaaaat cggtcagatc
     2941 ttccgccatt ttttttgtga gcgatgtgac aagaactttt cccccttctg aaatttcttt
     3001 tacaacttca tcataaaggg tatctatctc attttttgaa ggaagaattt gaggcatcgg
     3061 gtctaaaagt ccagtaggac gattgataag ttcaataact tcagatacat ggcttatctc
     3121 atattctcta ggtgttgcag aaacataaag aactctgtca agccggcttt caaattcttc
     3181 aaaattaagc ggacggttat caagtgcgct cggcaggcgg aatccgtagt ttacaaggac
     3241 atcttttctt gctctgtccc cgcgatacat tcctctgatc tgcggaattg ttgcgtgact
     3301 ttcatcaatt atgacaagaa aatcatcccc gaaataatca agcagggtag gtggtgcatc
     3361 acctacttca cggttagtta tatatcttga ataattttca attcctgagc aatatccggc
     3421 agtttcaagc attgcaacat cctgggtcac cctttcatag attcgttgtg cttcgatcag
     3481 tttattgttt ttctcaaaat atttcaccct ttcttcaagc tcggttctta tcaactcgac
     3541 actttttttc aaagtctctt tttcagctac ataatggctt gaaggatata ctctgacact
     3601 gtcgagattc ctgatctttt tcccttcaaa cggtttgatc agtgcaattt cttcaatttc
     3661 atcaccgaaa tatgagatcc ttatcgcaat ttcctcttca taaggaggaa atatctcgac
     3721 aacatcacct ttaaccctga attttccccg gttcagatcg aactgacttc tttcatactg
     3781 gatctcaaca agcccggcac aaagatcttt tagcggaaaa gtgttgccgg tgaaaagttt
     3841 caaagtaagt cctctgtatg catcaggaga accgatgccg taaattgaac ttaccgaagc
     3901 gacgatcacg cactgctcat tttcaagtaa gctcgcagtg gcaaagtggc gcattctgtc
     3961 aatgtcgtca ttccttgaag catctttttc gatatatgtg tcagttgtcg gaatgtatgc
     4021 ctccggctga tagtaatcat aaaacgagat gaaatatccg acattgtttt caggaaagaa
     4081 attcttgaac tcgccataaa gctgtccggc aagggtctta ttgtgtgcaa tgacaagagt
     4141 cggacggttt aatgccctga taacattagc cattacaaat gttttgccgg ttcctgtcgc
     4201 accgagtatc agttcattct ttgaaccgtt cttaaaccgt tcaataagct tttcaatagc
     4261 ctgcggctga tctcccgccg gcttaaacgc tgaatttaat ttaaattcca tgacaactcc
     4321 tcagaaatcc ggttgaatta tgccacgaaa acggaaaagg gcaaatattg agttcgtacc
     4381 gattgcaata acctaaaacg aaaatcccag cgcaatcccg taagtctttg aaacaggatc
     4441 aattattgga gcaatgctgc ttaaagtcag aattgagggt tctgtgttga gttgtttgta
     4501 tttcaaggat ttatacagaa ggatcgatcc gccagtgata gaaaggattg cgggagtaaa
     4561 ggagctcaat tgaagaaaat gtgttaaaac aagccctatg ttttcaaaag gcaccccgct
     4621 tgtagattgt tcttccggtt gtttgctttt ttctttcgtt aaataaatat tatagctgtc
     4681 ataaaacccg taaacagcca atgctgtcaa cgggatggac atcattaaca agccgattcc
     4741 tgcgttcctg aattttttag aagagt
//
LOCUS       DAOOYB010000224         4763 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_20837_length_4763_cov_5.997451, whole genome shotgun sequence.
ACCESSION   DAOOYB010000224 DAOOYB010000000
VERSION     DAOOYB010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4763
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20837_length_4763_cov_5.997451"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(367..642)
                     /locus_tag="PL056_11080"
     CDS             complement(367..642)
                     /locus_tag="PL056_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47601.1"
                     /translation="MGFSRRVIEVTPLDLSSTKRARKIIATHVKGEKSCYTSILYVEE
                     ETIKEHTVIMPSINEGVESAKNWIKENFSDCRFDDITGHNFEKYPLH"
     gene            complement(906..1919)
                     /locus_tag="PL056_11085"
     CDS             complement(906..1919)
                     /locus_tag="PL056_11085"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HPM47602.1"
                     /translation="MSDFNETDREFTNCPHCGEQILKKAIICKHCKKSVEMAECPFCA
                     ELNLKDVDVCAHCGGNLKKLVETVPAEKIGAKAPVNEKSEGFPVKRLMLFLIGLAVFF
                     ASFFAFVVFKVQDQWEINRMSEKCEKGDFQACDFIKYSFLCKEGSSDGCMQLGIIYCS
                     GVIVKKDGAKGLRLLQKSCDIAGDQGRCFALETLKEMCLGQDVKVNESSSGNWSNKSP
                     KTMNWSDAKKYCENLKEEGYSDWRLPTISELRTLIQNCPRTDTGGACKVTNDCLSSNC
                     WSNACERCYPRSSYSKLGDTEGFWSSSELSDREDRAWNVGFGNGDVSNIYKNLNHYVR
                     CVR"
     gene            complement(2125..3168)
                     /locus_tag="PL056_11090"
     CDS             complement(2125..3168)
                     /locus_tag="PL056_11090"
                     /inference="COORDINATES: protein motif:HMM:NF022633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2971 domain-containing protein"
                     /protein_id="HPM47603.1"
                     /translation="MQKTDFLEMWEIGEKISAADQSLIAGKTIEEIAKNLNISTEDME
                     SHIDFIKIGKFVKEVSEQQKYYRLSTDDQELFLKYLMSKSIYDGIMPITFFRYRPVNR
                     FLFDSLESGTFWFSEFDNLNDPLEGTDVRNYEILSDTDKIEHLRFLERKYGYLPPGTS
                     YSMSFEKYKIMLKSVTKKVRDQIMILSLTGTPDNPDMWSNYSGSGTGICIEIDPFEDV
                     KFFWTIHKVSYVNKLPSIIDSPKTMRLNLSGEYFQALACTKTLEWSYENEWRIVKLFN
                     DEKVNAQPFKKSMIKSIIFGFNTSSEDKEKIKNIVSNANGYRNVIFRRIFNNEMKIVD
                     DLDGEDFVVMAKD"
     gene            complement(3295..3573)
                     /locus_tag="PL056_11095"
     CDS             complement(3295..3573)
                     /locus_tag="PL056_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47604.1"
                     /translation="MSDSKKFSFVELFYSGKDKLFIYANDSVVYFSPGVTCPTVPSSE
                     FERILKELDHYFINVPGTCPSGFSFTDLSNIKHYVAYELINHVKVHLV"
     gene            complement(3815..4654)
                     /locus_tag="PL056_11100"
     CDS             complement(3815..4654)
                     /locus_tag="PL056_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47605.1"
                     /translation="MKNVRFLRLLLVVSLFFSFLLFTGCDGGTTDKKTKKYGSEGGYC
                     YPDGTCDEGYECNETMNMCFEAEINDTGDTDDTGNTGDTGNSGDSGDTGNSGNTGNSG
                     DTGNTGNDVCEEWNNGEIRNCKDFDSFERCTDGSWVTVLLAHKGLEWSCVAPEDLSLA
                     DAISYCKDIGGHLPNISELRTLIKGCSATITGGSCGVTDECRDINSCWSVACDGCSHD
                     DDSGKYSIFGDTSWFGSGYFWSSSVLSIQGSDSNNAWYVFFDFASLGYNPVSYESPVR
                     CVR"
BASE COUNT         1526 a         1025 c          712 g         1500 t
ORIGIN      
        1 ctatttatgt tttattgcaa taaaattgag atacgattat ttaagtcaat ggaaaacaga
       61 agaaaagcgg aattgattcg gggagctggg agggttttac gacaaaaaca tatgtagtta
      121 tggcgacccg acacttatgg tttgcgaccc ttatggtttg cgtggcgacc cttatggttt
      181 gtgaccctta tggatgataa aataggaatt acgggacatg aaaatttaga tagccccctg
      241 atctccagga acggttttca gggggttttg ttttaattcg ctatagcaaa attcactaaa
      301 gcaaatttcc gtcagctgcc tgtaggaaga tcggagatca gttcattcag ggaaggaggg
      361 gacatattaa tgaagtggat atttctcgaa attatgaccc gtaatgtcgt caaaacgaca
      421 atctgagaaa ttttctttaa tccaattttt agctgattct actccttcgt taattgaagg
      481 catgattact gtatgttcct taattgtctc ttcctcaaca tacaaaattg aagtataaca
      541 acttttttcg cctttaacat gcgttgcgat aatttttctt gctctctttg tagaacttaa
      601 gtctaaggga gtaacttcga taactcttct tgaaaaaccc attttctcct cctttgtaaa
      661 aaacaaatta ctttccgctc aatacacacc ttaaaaaaac cctgcaaaaa catgataatt
      721 aaaattaaaa atctgtcaat ttatttctta tatttctgta ataatttcag tggtttaatt
      781 agtttcatgg cctaaaacta atagttttag cgaaatccga tatatttcca cagatgtcac
      841 ggggaaatga tagacttatc ggaaacgaaa aagcacacat ataattataa gtccacgcag
      901 agacatcaac gcacgcagcg gacatagtgg ttgaggttct tgtagatgtt gctcacatcg
      961 ccattgccga aacccacatt ccatgcacgg tcttcacgat ctgatagctc cgaagacgac
     1021 cagaaccctt ctgtgtctcc gagctttgaa tatgacgagc gggggtaaca tctttcacaa
     1081 gcattgctcc agcaatttga agataagcaa tcatttgtaa ctttgcatgc tccgccggtg
     1141 tcagttctgg gacagttctg aataagtgtt ctgagttctg aaattgtcgg caatctccaa
     1201 tctgaatagc cttcttcttt taaattttca caatatttct ttgcatcact ccaattcatt
     1261 gttttcggcg atttatttga ccagttgccg cttgaggatt cattgacttt aacatcctgc
     1321 cccaaacaca tttcttttaa agtctctaaa gcaaaacacc gaccctgatc accagcaata
     1381 tcgcaagatt tctgaagcaa ccttaatcct ttcgctccat cctttttaac tataacacca
     1441 ctgcaataaa taatacctaa ttgcatgcaa ccatcagaac tgccctcttt gcacaaaaat
     1501 gaatatttaa taaaatcaca tgcttgaaag tcgccttttt cgcatttctc tgacattcga
     1561 ttaatttccc attgatcctg cactttaaac acaacaaatg caaaaaaaga tgcaaaaaaa
     1621 accgccaatc caattaaaaa cagcatgagt cttttcacgg gaaatccttc acttttttca
     1681 tttaccggag cttttgcacc aattttttca gcaggaacag tttccactaa ttttttcaaa
     1741 ttgccgccgc aatgtgcaca cacatccaca tcttttaaat tcagctccgc acagaaagga
     1801 cattcagcca tctcaacact ctttttacag tgtttacata taattgcttt tttaagaatt
     1861 tgttcaccgc aatgagggca gttagtaaac tccctgtcag tttcattaaa atcactcatt
     1921 tatctctcca acaaaaaatt aaacacaacg gaagtatttt aatgaatcag aaagaaaagt
     1981 gaatttttat gcaattaaaa gttgccgtga gcgtttaacg gttttctcac tcgtcacggt
     2041 cgcaaagcga catagaaccg tagtaaaaac gaagtctgga agaaaagcaa atggaaggca
     2101 agtggaaaat ctcgaagcca aaaactaatc tttcgccata acaacaaaat cttcgccatc
     2161 aagatcatca acaatcttca tctcattatt aaatattctc ctgaatatga catttctgta
     2221 gccgttcgca tttgaaacta tattttttat tttttctttg tcctctgaag atgtattaaa
     2281 accaaaaatt atcgatttaa tcatactttt tttaaatggc tgcgcattca ctttttcatc
     2341 attgaaaagt tttactatcc gccactcatt ctcataactc cattccagtg tcttagtgca
     2401 agctaacgcc tggaaatatt cgccacttaa atttaatctc attgtttttg gagaatctat
     2461 tattgacggc agtttattga cataactaac cttgtgaatc gtccaaaaaa attttacatc
     2521 ttcaaaagga tcaatctcta tacatatccc cgtgccactg ccagaataat ttgaccacat
     2581 atcaggatta tcgggagttc cggttaaaga cagaatcatt atctggtctc gcactttttt
     2641 tgtgacactc ttcaacatga ttttgtactt ttcaaatgac atactgtagc tcgtgccagg
     2701 agggagataa ccatattttc tttctaaaaa tcttaaatgt tcaattttgt cagtatccga
     2761 caagatctcg tagtttctaa catcagttcc ttcaagagga tcattaagat tgtcaaattc
     2821 tgaaaaccaa aatgtgcccg attccaaaga atcaaaaagg aaacggttaa ctggtctgta
     2881 tctaaaaaag gttatcggca ttataccatc gtaaatactt tttgacatca aatatttaag
     2941 aaacagttcc tgatcatcag tagataatct ataatatttt tgctgttccg aaacctcttt
     3001 cacaaactta ccgattttta taaaatctat gtgactttcc atgtcttctg ttgaaatatt
     3061 gagatttttg gcaatctctt caattgtttt tccagcaatt agactttgat cagctgctga
     3121 tattttttca ccaatttccc acatttctaa aaagtctgtt ttttgcattg ccgaccccca
     3181 ttgagttccg agttttaata atcattttag ccacttacac caatcaacac attggcatgt
     3241 tgttttataa aaacaatcat tattaatttc ctccttaatt ataaaggggg cgccttaaac
     3301 taaatgtacc ttcacatgat ttatcagttc atacgcaaca taatgtttga tattgctaag
     3361 gtcagtaaaa gaaaacccgg acggacaagt acctggaaca tttataaaat aatgatcaag
     3421 ctcttttaat atccgctcaa attctgaaga gggaacagta ggacaagtaa caccaggtga
     3481 aaaataaaca acagaatcgt tggcataaat gaaaagctta tcttttccag aataaaacaa
     3541 ttcgacaaaa ctgaactttt ttgaatcact cataaaatta ctccttcaaa acaattaaac
     3601 tcccgctcaa caaacacttt aaaaaaaccc tgtggacatc ttatccaaaa cacgaattat
     3661 gtcaatgtgc attttataat tttatgagca tattgtattt ttttagtttc acggtttaaa
     3721 actaatgatt tttgcggaaa aacacacttc acatataaag ttaaactgaa atgaagttcc
     3781 taagattctt ttctcttcgc caacatgttc aatatcaacg cacacaccgc acaggacttt
     3841 cgtaagacac aggattatac cccaaactag caaaatcaaa aaacacatac catgcgttat
     3901 tcgaatccga accctgtatt gacaacacgg acgaggacca gaaatagccg gagccaaacc
     3961 agctagtgtc accaaaaatg ctatattttc cgctgtcgtc gtcatgagaa cacccatcgc
     4021 aagcaacact ccaacaactg ttaatatccc tgcattcatc ggtgaccccg catgatcctc
     4081 ctgttatagt ggcggaacaa ccctttataa gggttctgag ttcgcttata ttgggaagat
     4141 gccctccaat atctttgcag taactaatcg catcagcgag acttaagtct tctggagcaa
     4201 cacacgacca ctccagtccc ttatgtgcca gcaaaacagt tacccaagaa ccatcagtac
     4261 accgctcaaa cgagtcaaaa tctttacaat ttcttatttc gccgttattc cattcctcac
     4321 aaacatcatt tccggtgttt cctgtgtctc cagaattccc tgtattgcct gagttaccag
     4381 tatctccact atcaccagaa ttccctgtat ctcctgtatt tcctgtatca tcggtatctc
     4441 ctgtatcatt gatctctgcc tcaaagcaca tattcattgt ctcgttgcac tcataacctt
     4501 catcacatgt tccatcagga tagcaataac ctccttcaga accatatttt tttgtttttt
     4561 tatcagttgt tccaccatca caacctgtga acaataaaaa agaaaaaaac aagctaacaa
     4621 ccaataataa tcttaaaaac ctaacatttt tcatttttaa ctccctttaa gtgaccacat
     4681 atcaaaaaat tttagaaaac aggtacttaa agtcaagttt ttataacctt ttggttataa
     4741 ctaaaaggtt caatccgtgt aaa
//
LOCUS       DAOOYB010000225         4759 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_20859_length_4759_cov_9.552721, whole genome shotgun sequence.
ACCESSION   DAOOYB010000225 DAOOYB010000000
VERSION     DAOOYB010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4759
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20859_length_4759_cov_9.552721"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            42..272
                     /locus_tag="PL056_11105"
     CDS             42..272
                     /locus_tag="PL056_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47606.1"
                     /translation="MSLDIATINWHTSNGTTSGNYYIFNTSGVEGCDPGTYSAPNPDG
                     LAEAVALDFSLVPDNYRSACMDKNGTLKNNSI"
     gene            343..765
                     /locus_tag="PL056_11110"
     CDS             343..765
                     /locus_tag="PL056_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPM47607.1"
                     /translation="MKKGFTIIELMVVIAIIGVLATVAIPMYSDYSKKSKVSEIPMTL
                     KMIIQQQIGRREDPLAGGNYVTDLATIAWTTSNGTTEGRYYGFGTSGVEDCDPGDGNP
                     PLPIGLAEAWALQPTEVDPNWKSICMDMDFNLLKNGDI"
     gene            840..>2075
                     /locus_tag="PL056_11115"
     CDS             840..>2075
                     /locus_tag="PL056_11115"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HPM47608.1"
                     /translation="MSLLFINPFVEDFTAYNLWAVPLGLFRLMEHFEKVGEVVEFVDL
                     LDGFDVGGDGAVAPEFRSWGRHSYWKKRIEKPVEVKFVPRNFNRFGASDERAYEILSK
                     VNKPEKIFVSSGMTYWYKSTVRTIKIVRDVFPDVEIVVGGIAATLIPEFFRMEGVKVH
                     EGIYPVGEDITGTGSKYLNDLRFFPANIVKGCPNRCSYCASSIFDESIKIKDIQALSQ
                     NLEKWHGVTNFVDVAFYDDALLLNKGLYLKDFLNTLTPGKFRFHTPNGLHLKEVDEEL
                     CRYFADYNFPQLRFGFETAFSRFDDKTNFVELTKVVEMLHKYGFNHEMIGVYLLCGLP
                     GQTVEEVEKTIELVSAIGARPYLSEYSPVPKTALFKKHLAESRLDFENEPLYQNNSAS
                     SFRSPVFTPNVMKGLKEKLN"
     assembly_gap    2076..2175
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2246..2995
                     /locus_tag="PL056_11120"
     CDS             2246..2995
                     /locus_tag="PL056_11120"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPM47609.1"
                     /translation="MGKKVVLLVDDNELNRKVIAKIVESLGYETISAGSGVEALQIFN
                     HDKPDMILMDCRMPDMDGFDTTKIIRQIENVNDMKHIPIIAVTADDKSEEWERCQNAG
                     MNDFIGKPVDVAKLSEMMKKYSFEPGDNANVDLNALLVSADNDPVWAKELLVMFVADT
                     VERIEEIEKMIEEKKFSKDDAIRHFHTIKSSAASIGAFELSTLAKKMENFVKNGEVTN
                     ITDNFQQLKDEFKRSATVLENGIAQKINEKS"
     gene            2982..3950
                     /locus_tag="PL056_11125"
     CDS             2982..3950
                     /locus_tag="PL056_11125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HPM47610.1"
                     /translation="MKNLKVAAGFLITALLFYFLLRDLDFELFFSYLIKGDIVLILLG
                     IAVYLLSFLIRGLRWKILLKHLGSFKVNDLAMLEIAGYAVNNVFPVRIGEFTRAWITG
                     KRNSISRTSVLASIFVERIFDGLTIALILSATLFFYPFPQNVKTLAVMASAMFALLFL
                     FIMFGTFSDKPLKLIRMLKSKLPEFTGFIFDIAEKFLKGASSLNSLSQISAVTFLSIL
                     VWVFELTVYILISRAFEVDIPFAGYLFMLCAANLGMLAAPTPGGLAVFQAAIVFALRS
                     FTILYEKRMAVAIILHMAQIIPVTIIGLMWLYLNHISVLSAQNDTD"
     gene            4024..4497
                     /gene="greA"
                     /locus_tag="PL056_11130"
     CDS             4024..4497
                     /gene="greA"
                     /locus_tag="PL056_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019953512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HPM47611.1"
                     /translation="MAGIPITKEGFERISEELKHLKKVERPDIIKKIAEARSHGDLKE
                     NAEYHAARERQSFIEGRIGYLEGVVADCEVIDVSKIKGDKVFFGATVTLMDVDTEQHV
                     KYTLVGEPESDPKNGRISVASPIGRAVLGKSEGDDVKVQTPSGVREYEIIEVKYS"
     gene            4497..>4759
                     /locus_tag="PL056_11135"
     CDS             4497..>4759
                     /locus_tag="PL056_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47612.1"
                     /translation="MINDLSANNRTLTFLNQPVASVAGLSKQQLNKISKALGEKVFNL
                     VIENIPEKYEARLLANSIADLVQGEMSTLRAKVLGWKRGFGKSP"
BASE COUNT         1342 a          783 c         1127 g         1407 t
ORIGIN      
        1 taaacagggg taattttaat tgtcgtcaga ggtaattata gttgtcgctg gacatcgcta
       61 ccattaactg gcatacttca aatggcacta cttctggaaa ctattacatt ttcaatacat
      121 ccggtgttga aggatgtgat ccaggaactt attcagcacc caatccagac ggacttgcgg
      181 aagcagtcgc tctggatttt tcgctagttc ccgataacta cagatctgcc tgtatggata
      241 agaatggaac tttaaaaaac aactcaattt aagtatttga ttctcattgg tacatttttt
      301 gcaacaagca ttgtgatttt aggattaaga atgaggtgta taatgaaaaa agggtttaca
      361 attattgaac tgatggtggt tattgcaatt attggtgttt tggccactgt agcaatccca
      421 atgtattctg attattcaaa aaaatcaaaa gtttctgaaa taccaatgac cttaaaaatg
      481 ataattcaac agcagatagg tcgaagggaa gatcccttgg caggaggaaa ctatgtgacg
      541 gatcttgcaa caattgcgtg gacgacatca aatggaacta ctgagggaag gtattatgga
      601 tttgggacaa gcggtgttga agattgtgac ccgggagacg gcaatccacc tcttccaatc
      661 ggtcttgctg aagcttgggc tttgcagccc actgaagttg atccgaactg gaaatcaatt
      721 tgtatggaca tggattttaa tcttttgaaa aacggggata tttgattttt tccttcccat
      781 tcccaaattt ttctgatagt tttgtctggc agtttagttg tttatagttg agttgttgaa
      841 tgtcccttct tttcataaat ccatttgttg aagattttac tgcgtataat ttgtgggcgg
      901 ttcctcttgg gttgttccgt ctgatggagc attttgagaa agttggggaa gttgttgaat
      961 ttgttgatct tcttgatggc tttgatgtgg ggggtgatgg ggcggttgct ccggaattca
     1021 ggtcttgggg tcgtcattct tactggaaaa aaaggatcga aaaacctgtt gaagttaagt
     1081 ttgtgccaag gaatttcaac cgtttcgggg cgagtgatga gagggcttat gagatattgt
     1141 caaaggtgaa taagccggaa aagatctttg tttcaagcgg gatgacatac tggtataaaa
     1201 gtacggtgcg gacaataaag atcgttcgtg atgtttttcc tgatgttgag attgtggttg
     1261 gtgggattgc ggcgactttg atccctgaat ttttcagaat ggagggggtg aaggttcacg
     1321 aggggattta tccggttggt gaagatatta cggggactgg ttctaaatat ttgaatgatt
     1381 tgaggttttt tcctgcaaat attgtaaaag ggtgtcccaa caggtgcagt tactgtgcaa
     1441 gctcgatttt tgatgagtca ataaagatca aagatattca ggcacttagc cagaatcttg
     1501 aaaaatggca cggtgtgaca aattttgtcg atgtggcatt ttatgatgat gcacttttgt
     1561 tgaataaggg tttgtattta aaggattttc taaacacgct gactcccgga aaattcagat
     1621 tccatactcc gaatggcctg catctgaaag aggttgatga agagttgtgc agatattttg
     1681 cggactataa tttccctcag ctccgtttcg gttttgaaac ggccttttca agatttgatg
     1741 ataagacaaa ttttgtcgag ttgacaaaag tggttgaaat gcttcataaa tatggtttta
     1801 atcacgagat gataggggtt tatcttctct gcggactgcc gggccagaca gttgaagagg
     1861 ttgaaaagac gatagagctt gtttcagcca tcggtgcaag accgtatctg agtgaatatt
     1921 caccggttcc aaagaccgca ctatttaaaa agcaccttgc tgaaagccgt cttgattttg
     1981 aaaatgagcc gttatatcag aataattcag cgtcaagttt cagaagtccc gttttcactc
     2041 caaatgttat gaaagggctg aaagagaagt tgaatnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnatttt ttttggattc atttaataaa ttgtgatagt atcggtgact
     2221 aaagtgtttt ctttccggag atgttatggg aaaaaaggtt gtgcttctgg ttgatgacaa
     2281 tgagttgaat cgaaaggtta tagcgaagat cgttgagtcg ctcggatatg aaacgatatc
     2341 ggcgggaagc ggagttgagg cgcttcagat attcaatcac gataagccgg acatgatcct
     2401 gatggattgc cgtatgccgg atatggatgg tttcgacacg acaaagatca ttcgtcagat
     2461 agaaaatgtt aatgatatga agcacatacc cattatcgct gtgactgcgg acgataagag
     2521 tgaagagtgg gaaagatgcc agaatgcagg gatgaacgat tttattggaa agccggttga
     2581 tgttgcaaaa ctttcggaaa tgatgaaaaa atattctttt gaacccggtg ataatgcgaa
     2641 tgtcgatctg aacgcactgt tggtttcagc agataatgac cccgtttggg caaaagaact
     2701 tcttgtgatg tttgtggctg atactgtcga aaggatcgaa gagatcgaaa agatgatcga
     2761 agagaagaaa ttcagcaaag atgatgcaat aaggcatttt cacacaataa aaagttctgc
     2821 cgcaagtatc ggtgcctttg aactgagcac acttgcaaaa aagatggaaa actttgtcaa
     2881 gaatggggaa gtcactaata ttactgataa ttttcagcag ttgaaagatg agttcaaacg
     2941 gtctgcaaca gttcttgaaa atggtatagc ccagaaaata aatgaaaaat cttaaagtcg
     3001 ccgcagggtt tctgataaca gctcttcttt tctattttct tttgcgtgat cttgatttcg
     3061 agttgttttt ttcatatctt atcaaaggcg acattgtcct tattctgctt ggcattgcgg
     3121 tatatctttt gtcatttctg atcaggggct tgcggtggaa gatccttctg aaacatctcg
     3181 gcagcttcaa ggtcaatgat cttgcaatgc tcgaaattgc cggttatgct gtgaacaatg
     3241 tgtttccggt gaggatcggt gaattcaccc gtgcatggat cacagggaag agaaattcaa
     3301 tttcaagaac atcggtactt gcttcgatat ttgttgagcg tatttttgac gggttgacta
     3361 tcgccctgat actttcggca acactttttt tctatccttt cccgcagaat gtaaaaacgc
     3421 ttgcagttat ggcttcagca atgttcgcgt tgctttttct tttcataatg ttcggcactt
     3481 tttcagataa gccattgaaa ttaataagaa tgttaaaatc aaaacttccg gaattcacag
     3541 gtttcatatt tgatattgca gagaaatttt taaagggcgc cagttcactt aattcgttaa
     3601 gccagatatc cgctgtgaca tttttaagca ttctggtctg ggtttttgaa cttactgtat
     3661 atatccttat atcgagagct tttgaagttg acatcccttt tgccggttat cttttcatgc
     3721 tttgtgccgc aaatctcgga atgcttgccg ctcctacacc cggcgggctt gctgtcttcc
     3781 aggcggcgat agtttttgca ctgagatcgt tcaccatact ttacgaaaaa aggatggcag
     3841 ttgcgatcat tctccacatg gcccagataa ttccagtcac catcatcggt ctgatgtggc
     3901 tttatcttaa tcatatatcg gtgttatcgg cgcagaacga caccgattga tcggcgaaaa
     3961 cttgattaga gcaaaaacag atatataatt cgaatcccgg attttttaat taggaggtct
     4021 gaaatggcag gaattccgat cacaaaagaa ggatttgaaa ggataagcga agagctgaaa
     4081 catcttaaaa aagttgagag acccgacata atcaaaaaga tcgccgaagc gagaagtcat
     4141 ggagatctca aagaaaatgc cgaatatcac gcggcaaggg aaaggcagag tttcattgaa
     4201 gggcgtatcg gctatcttga aggtgtggtc gctgattgtg aggtgatcga tgtttccaag
     4261 ataaaaggag ataaagtgtt tttcggtgcg actgtaactt tgatggatgt tgatactgaa
     4321 cagcatgtta aatatacact tgtcggagag ccggaaagtg atccgaaaaa cggcagaata
     4381 tcggttgcaa gcccgatagg aagagcggtt cttggaaaat ctgaagggga tgatgttaaa
     4441 gtccagacac cgtcaggagt cagggaatac gagataatcg aagtcaagta ttcgtaatga
     4501 tcaatgatct ttcagcgaat aacaggacgc tgacatttct gaatcagccg gtcgcatcgg
     4561 ttgccggact ttcaaaacag cagttgaaca aaatttcaaa agcacttggc gaaaaggttt
     4621 ttaatcttgt gatcgagaat attcctgaaa aatatgaggc gcggcttctt gcaaattcta
     4681 ttgcggacct tgttcaggga gaaatgtcaa cgctcagagc gaaagttctc ggatggaaaa
     4741 ggggtttcgg aaagtcacc
//
LOCUS       DAOOYB010000226         4743 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_20967_length_4743_cov_15.036903, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000226 DAOOYB010000000
VERSION     DAOOYB010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4743
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20967_length_4743_cov_15.036903"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1263
                     /gene="serS"
                     /locus_tag="PL056_11140"
     CDS             <1..1263
                     /gene="serS"
                     /locus_tag="PL056_11140"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013638750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HPM47613.1"
                     /translation="KLILENKDKVLKSLMNRNFKDLSLIDEIVELSEKRKTLVTESEH
                     ARATQNQRSKEMPLIMKNGTEEEKSAIREELKVLSEKVKAYGPEVKAVEDKINSILMS
                     IPNMIADDAPIGKDETANPVVKVWGDKPEFSFKPKEHFDVATQTIDLERGVKLSGSRF
                     VVYKKELAMLERAVMNYMLDNAHKNGYTEIVPPVLVSRETMTGSGQLPKFEEEAYKTT
                     DDMFLIPTAEVSLVNLHRDEILNGADLPLKYAAYTPCFRREAGSYGKDMKGIIRLHQF
                     NKVELVKFTKPEESHTELLKLLGNAEEILQGLGVHYRIIELCSGDLGFNSLKTFDIEV
                     WLPGQNTYREISSCSNTGDFQARRAGVRFKRDAVNKPEFAHLLNGSGLAIDRTLVAII
                     ENYQQADGSVKIPEVLRKYMHGLEVLSL"
     gene            1260..2123
                     /locus_tag="PL056_11145"
     CDS             1260..2123
                     /locus_tag="PL056_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47614.1"
                     /translation="MTLISFRQNAAAVFAIILTIWALHSFFSWHTAPTPEEIPEKISE
                     SLEILHGEPVFLSSQLLDKLVLNHPEINFFPAGGNSLKNAKTSGLFYILQKYQTLDCS
                     DIDRELKSEIIAHTTGFTLKKCYSSQNSNIVYASSFIEKMTVTTDDFEIPAKFINGQF
                     RTGPSGWQKIETGSAEFGDDFRTAVSAHPLPDKKKITIVIPSVDINTKKIVLGAGIAN
                     SGVFKGSKPVIITAFQNSLKKEIKSADGKWVEHELKNFSGSEPVTITVHTEKSGKRHF
                     YFDLKYFEAEK"
     gene            2120..3712
                     /locus_tag="PL056_11150"
     CDS             2120..3712
                     /locus_tag="PL056_11150"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPM47615.1"
                     /translation="MRNLFKNCDIKVFLTMSALAVFYLFMLVYQSRQVAITDDARFYM
                     AAAKSYYSYFDNCKFSKKCFEKGTIDRYWRENNEHPPFAKLLMAGGYGFFHKKLNILD
                     EIRALRIGISIFAVILLMFIFSFTRRAFSFHAAVFASLSFIFLPRTFFHARVATLDFA
                     VAGTSFIFVYCYWRGFTSKFWAWMTGVAFGIALATKLNAPFMILPVLIHFFIFKWSDI
                     KKRPFKTVFAPQFVSMLIISLPLFFMMWPWLWHDSAARFISYVKFHTNHYGILMYYLG
                     KIYGEPRPPWHAPFVMMVLTTPLISLFSASLAPVIYKWKNESENNFAMTLSILCAFVS
                     IAVLMFMPAPFYSGVKLFQPFFPFLAVIAGTGIYSMLKSVTFIPEKYKFAPGILFFIP
                     VIVSMVDLKNDHLSYYNELAGGTKGASLYKNEQHYYDLFYYDLTEFFNKECEKKRCRV
                     AFEPNGKEYDPSSGILKKAGYLTKNFIYSDLAKADYFVLTHEYRWKDYPNILRRSKNL
                     KKVYSIEKQGVELLTVFKLNSK"
     gene            complement(3724..4572)
                     /locus_tag="PL056_11155"
     CDS             complement(3724..4572)
                     /locus_tag="PL056_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="EB domain-containing protein"
                     /protein_id="HPM47616.1"
                     /translation="MKKFFKFMSIVVFAALFLVSCGDDKKTVKTDEDTAETIDEELTD
                     NEVSDETVVDEEEVADVVDEEEVLDEDIIDPCAEKECPENSTCVADGDEATCECNEGF
                     HPTNGECISDTELTEIGAFTVSFKGAVNQTSNPSEISPGTGDAEFTYFDDDFTFTVLH
                     IDSEQLKIDFPFAAVAEGGMVAATWIDAVSFSPKFFAVNVPDNLTVGNDIDFVETQTY
                     ALYGDLAYDGSTVGIKCIRAVSGDATLDLVELTATDIEFSAEGKLIDPAYALEQGYNL
                     PEICED"
BASE COUNT         1432 a         1038 c          926 g         1347 t
ORIGIN      
        1 aagctgatac tggaaaacaa agacaaggtg ttgaaaagtc tgatgaatcg taattttaag
       61 gatctatcgc tgatcgatga gatcgttgaa ctgagcgaaa aaagaaagac gcttgtgacc
      121 gagtccgaac atgcaagggc aacgcagaac cagcgttcaa aagagatgcc gctcatcatg
      181 aaaaacggca ctgaagaaga aaaatccgca ataagggaag aactcaaggt gctcagcgaa
      241 aaagttaaag catacggccc tgaagtgaaa gcggttgaag ataagataaa ttcaatactt
      301 atgtctattc cgaacatgat cgctgatgac gctccgatcg gaaaagacga aacggcaaat
      361 ccggttgtaa aggtttgggg tgataagccg gaattttcat tcaaaccgaa ggaacatttt
      421 gatgttgcga cacagaccat tgaccttgaa agaggtgtca aactcagcgg ttcaagattt
      481 gtagtctata aaaaggaact tgcaatgctt gaaagggctg taatgaacta tatgcttgat
      541 aatgctcata aaaacggtta taccgaaata gttccgccgg tacttgtaag ccgggagaca
      601 atgaccggtt caggtcagct tcccaaattt gaagaagaag catataaaac aactgacgac
      661 atgttcctca tcccgaccgc cgaagtatcg ctcgtcaacc ttcacaggga cgaaatactc
      721 aacggagccg atcttcccct taaatatgcg gcttacactc cctgcttcag aagagaagcc
      781 ggcagttacg gcaaggatat gaaaggaata atccggcttc accagttcaa caaagtggag
      841 cttgtcaaat tcaccaaacc tgaagaatca catactgaac tcctcaaact tctcggaaac
      901 gctgaagaga tcctgcaggg gcttggcgtt cattaccgga tcattgaact ctgtagcggt
      961 gatctcgggt ttaactccct gaaaacattt gatattgaag tgtggcttcc gggacagaac
     1021 acttaccgcg agatctcaag ctgttcaaat accggcgatt tccaggcaag aagggccgga
     1081 gtcagattta aaagagatgc cgtcaacaaa cctgaatttg ctcaccttct taacggatcg
     1141 ggacttgcga tcgacagaac acttgttgca ataatcgaaa attaccagca ggcggacgga
     1201 tcagtgaaaa tccctgaagt tctcaggaaa tatatgcacg gtcttgaggt tctttctctt
     1261 tgacattaat atctttcaga cagaacgcgg cggcagtttt tgcaataatt ctgaccattt
     1321 gggctctcca cagttttttt tcatggcata ccgcaccaac ccctgaagaa attcctgaaa
     1381 aaatatctga atcacttgaa attctgcacg gggaaccggt ctttctttca tcacagcttc
     1441 ttgataaact tgttctcaac catcctgaaa taaatttctt tcctgctggc gggaactcac
     1501 tgaaaaatgc gaaaacctca ggtcttttct atattcttca aaaatatcag acacttgact
     1561 gcagtgacat cgacagagaa cttaaaagcg agatcattgc acatacaact ggtttcactc
     1621 ttaaaaaatg ttattcatct caaaattcaa atatcgttta cgcttcgtca ttcatagaaa
     1681 aaatgacagt cacaactgac gattttgaga ttccggcaaa attcataaac ggacagttca
     1741 gaacaggccc aagcggctgg cagaagatcg agaccggttc agcggaattc ggagatgact
     1801 tcagaaccgc tgtttcagcc catcctcttc ccgacaaaaa aaagataaca attgtaatac
     1861 cttccgttga tattaataca aaaaagatcg ttctcggggc cgggatcgcg aactcaggcg
     1921 tcttcaaagg atcaaagccg gtcatcataa ccgcttttca gaacagtttg aaaaaagaga
     1981 ttaaaagtgc tgacggaaaa tgggtggaac acgaattgaa gaatttctca ggaagtgaac
     2041 cggttacaat aacggtccac accgaaaaat cgggcaaaag acacttctat ttcgatttga
     2101 aatattttga ggccgagaaa tgagaaatct ctttaaaaat tgcgatatta aagtttttct
     2161 cacaatgtcg gcacttgctg ttttctatct ttttatgctt gtctatcaat ccagacaggt
     2221 cgcaatcaca gatgacgcaa gattttatat ggctgcggca aaaagttatt actcctattt
     2281 tgacaactgc aaattttcaa aaaaatgttt tgaaaaaggg acaattgaca gatactggag
     2341 ggagaacaat gaacatcccc cttttgcaaa acttctaatg gcgggaggat acgggttttt
     2401 tcacaaaaag ctcaacattc ttgacgaaat aagggctctt cgtatcggca tttcaatttt
     2461 tgccgttatt cttctcatgt tcattttcag ttttacaaga agggcttttt cattccacgc
     2521 cgcagttttt gcatcgcttt cattcatatt tctgccgaga acttttttcc acgcccgtgt
     2581 tgcaacactc gattttgccg ttgccggaac atctttcatt ttcgtttact gctactggcg
     2641 gggatttact tcaaaattct gggcatggat gaccggagtc gctttcggca tagcccttgc
     2701 aacgaaactc aacgctccgt tcatgatcct tccggtgttg atacatttct tcatttttaa
     2761 atggagtgac attaaaaaaa gaccgtttaa aaccgttttt gccccgcaat ttgtttcgat
     2821 gcttattatt tcgcttcctc tctttttcat gatgtggccg tggctctggc acgattcagc
     2881 cgcaaggttc ataagctatg tgaaattcca taccaatcat tacggcatat tgatgtatta
     2941 tcttggaaaa atatacggcg aaccgagacc tccctggcac gccccttttg tgatgatggt
     3001 gctcacaact cctttaattt cactattttc cgcatcactt gctccggtta tttacaagtg
     3061 gaaaaatgaa tctgagaata attttgcaat gaccctttcg atactttgtg ctttcgtttc
     3121 gatagctgtt ttaatgttca tgccggcacc tttctacagc ggtgtgaagc ttttccagcc
     3181 gttctttccg ttcttagcgg ttattgcagg aacaggaatt tattcgatgc ttaaaagcgt
     3241 aaccttcatt cctgaaaaat ataagtttgc tcccggaatt ctctttttta tccctgtcat
     3301 tgtctcaatg gttgacctta agaacgacca cttgagctac tacaatgaac ttgccggagg
     3361 gacaaaaggg gcctcacttt ataaaaatga acagcattac tacgatcttt tttattatga
     3421 ccttacagaa tttttcaata aagagtgtga aaagaaaaga tgccgtgtcg catttgaacc
     3481 caatgggaaa gaatatgacc cttcatcagg aatacttaag aaagccggat acctgacaaa
     3541 gaatttcatt tacagtgatc tcgcaaaggc cgattacttt gtcttaactc acgaataccg
     3601 ctggaaagat tatcccaaca ttttaagaag gtctaaaaat cttaaaaagg tctactctat
     3661 cgaaaaacag ggcgttgaac tcctgacagt ttttaagttg aactcaaaat aaaaaaaatt
     3721 tttctaatct tcgcatatct caggcagatt atacccttgt tctaaagcat aagcaggatc
     3781 gataagtttt ccttcagcgg aaaattcaat gtcagttgca gtcagttcaa caagatcaag
     3841 cgtcgcatca cctgaaacgg ctctgataca ttttatcccg acagtgcttc cgtcataagc
     3901 aaggtcgccg taaagagcgt atgtctgagt ttcaacaaaa tcgatgtcgt tcccgacagt
     3961 cagattgtca ggaacattga ctgcaaagaa tttgggtgaa aaggaaactg catctatcca
     4021 tgtcgccgca accattcccc cttctgcaac agcggcaaac gggaagtcta tcttaagctg
     4081 ctcactatca atatgcagca ccgtgaatgt aaaatcatca tcaaaatatg taaattcggc
     4141 atcgcctgtt cccggagata tctcggaagg gttgcttgtc tgatttactg cacccttgaa
     4201 actcacagtg aatgctccga tttcagttaa ttcggtatct gaaatgcact caccgtttgt
     4261 cggatggaac ccttcattac attcacatgt cgcttcgtct ccatcggcaa cacatgtcga
     4321 attttcagga cactcctttt cagcacacgg gtcaattatg tcttcatcaa gaacttcttc
     4381 ctcatcaaca acatcggcaa cttcttcctc atcaacaact gtttcatcgc tcacttcatt
     4441 atccgtcagt tcttcgtcaa tcgtttctgc tgtatcttca tctgttttaa ctgttttctt
     4501 gtcatctccg caggaaacaa gaaataaagc ggcaaaaaca acgatagaca taaatttgaa
     4561 aaacttcttc ataactaacc cccgttttta attaagtttc acacacagca ctgatattct
     4621 gctctaatta agaaaaaatt caagaaagtt gttatttcag tagcttcaaa aacatttcaa
     4681 ttgcctgtga aacgcctgaa tttttgtggt cagcgactgt tgtgtatatt ttcttgagat
     4741 ttt
//
LOCUS       DAOOYB010000227         4715 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_21137_length_4715_cov_12.529399, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000227 DAOOYB010000000
VERSION     DAOOYB010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4715
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21137_length_4715_cov_12.529399"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="PL056_11160"
     CDS             complement(<1..250)
                     /locus_tag="PL056_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47617.1"
                     /translation="MKLKYIPVLIVTVFLFSCTSCNESKPSNDKESTIDQDNNTQFDD
                     DPTDPDVDIADADLKDDNKLDEIPDIDEDTYCPPLKEAG"
     gene            complement(262..4572)
                     /locus_tag="PL056_11165"
     CDS             complement(262..4572)
                     /locus_tag="PL056_11165"
                     /inference="COORDINATES: protein motif:HMM:NF014466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HPM47618.1"
                     /translation="MRRVLFAIIWSLIFFEIYGWNEDFHSYCLDDNKTCEYFGNYNGH
                     QAITRESYWEIEKRGVIFPYFLSDPGNLMEDKLIYLLYGAAFADEPWLGRPENPYGRN
                     SGVLSNIDPEIFLGNIYSSDEYKISETDNGRVEVRFLRREAGSKRPNYYMGIYNFLEV
                     GGKNHFDGDYAADNYLHFSNKEIGGNGVVILRQENAVGNTGIGDGAWVFVNSEIGAAK
                     YGTILFGLARKFWEANVNEPPSFNDMVLASNCLDDDIVPATGSAIMDTWKMWSVDRRW
                     ASLPSTWLGGQPFICKPNRSYDPTSTNCSDRDLCASGTPSWPIWMPDNYDENNIDEFI
                     EELTNPFPSKSNRVAMIYLGWALHFIQDLAMPYHAINWSGEMHQWREKTFDNMLKNGD
                     FGKYGGPDMLKKRLDALFGIAPNIFSNKEICQRLGIYDEEGNYLESGVYDLFDSVRDK
                     SKGKAGGYENTTEDNVEFYIDAVEASVKLMTCLQYGMDDDNDGFDNSDDNCPNIYNPD
                     QADYDEDGVGDVCDPCPMMGSISIYNPREDDDKDGIPNGCDNCPNVKNKFVVASLNDE
                     IISAKTQECDDDDNWYECLLKLSFSGGASKNQERKNDGYFGNKCIVNFMGNQWLCRKV
                     WYWQPDHDLDGTGDACDTDHSSVTKLISTTSSNPLFFRGKSEVNRFVDLNYKVIGDTT
                     PGSTAKYCWMDLELMDEWGKGGYCTTSDLGKGYPTINDSSFGYSHGGDPEPYNRQLNK
                     LAWQTPKISSFMGKLSVVNKTKDSKSAIEKWDWTKNLQKDYERLYTTYVSSGQQNLKE
                     PFINYAASVGSAGTENCSADEDYYDEEEEFINPNCFRNVLYINARTKRENKQGTKLSY
                     YTENLRQNFFPFKITPEMLNSSERSNSTCSGCVKFSDAVSSVTELWIYKDNILKSEKR
                     KLNNADNIIATFNDEGSIISVFEGENEIYFGINDEDNPSEIILAGIAPKPQEDLIKGK
                     GAVAKGRIFLTSANMLYSLEKDDSGQQTNIGSGMTYRFREIMQMPYPENEINILSAGR
                     ELVMLRNNGQYYEPYILSGDTFIEIESDEYPVSRDSFSFSSDNWKMYIAGGVTDGREG
                     VAVHSDVWALDTAYGWRKIADSINVDMGDVLIKEENGILNIFSRTFPQKEVATAFINI
                     ATGETDYGKTVLESEFEAMDIEKYCISRKNDNTYPGKEFRNICSPAEDYQYKSYTFLD
                     YKFSLAGIDDFVFSGGLSGIRTFRINAAGGLENGDLDVVGTVNSIAVKDNALYSSRSN
                     KVFVFEVKENGDLVKRKEISASGCDQIRVSGNMLFTGENGKVNIYDITDPFNPVKKHA
                     VSLSMNVVDLEVWNEYLFVFHDKWFSSSKLAMFKFSDDGTIVKTDEIKLSCSDPEFMS
                     DSQNVYLGCKNGQKKIEFNESEKLVIKSIGGSKNYFRDTYLRNGIIYTVHSGRIFLSK
                     "
BASE COUNT         1308 a         1095 c          703 g         1609 t
ORIGIN      
        1 agcccgcctc cttcaaaggc gggcagtatg tgtcttcatc aatatcggga atttcatcca
       61 gtttgttgtc atccttcaga tcagcatcgg caatgtcaac atcaggatca gtaggatcat
      121 catcaaattg tgtgttatta tcctgatcta tggtgctttc cttatcattt gacggcttgc
      181 tttcgttgca tgatgtgcag gaaaacagaa ataccgtcac aattaaaacc ggaatatatt
      241 tcaatttcat tgcctcacct ctcatttgct caggaatatc ctgccgctgt gaactgtgta
      301 tattataccg tttctaagat aagtgtctct aaaataattt ttgcttcctc cgatgctttt
      361 tattacgagt ttctcacttt cgttgaattc aatctttttc tgtccgtttt tgcagcccag
      421 atatacattc tgactgtcgc tcatgaattc aggatcactg caactcagct taatttcatc
      481 agttttaaca attgttccgt catcagaaaa tttgaacatg gcaagctttg aactgctgaa
      541 ccatttgtca tggaaaacaa aaagatattc attccagacc tcaagatcaa caacattcat
      601 tgaaagagag acagcatgtt ttttcacagg attgaatgga tcagtaatat cataaatatt
      661 tacttttccg ttttctccgg tgaacagcat atttccagac acccttatct ggtcacatcc
      721 gcttgcactg atctcttttc tttttacaag atcaccgttt tccttcactt caaaaacaaa
      781 aactttgtta cttcttgatg aataaagtgc attatcttta actgcaatgc tgttcactgt
      841 gccgaccaca tcaagatctc cgttttcaag tccgcctgcc gcatttatcc tgaaagttct
      901 tattccgctg agtccgccgg aaaaaacaaa atcatcaatt cccgcaagag agaatttata
      961 gtccagaaat gtatagcttt tatactgata atcttcagca ggactgcaaa tgttcctgaa
     1021 ttccttcccg ggataagtat tgtcattttt tcttgatatg cagtattttt caatatccat
     1081 tgcctcaaat tcactttcaa gaacagtttt tccgtaatct gtttcacctg ttgcaatatt
     1141 tatgaatgct gtggcgactt ctttttgagg aaatgttctt gaaaagatat tcaggattcc
     1201 gttttcctct tttatcagga catcgcccat atcaacatta atgctgtcgg caatttttct
     1261 ccagccgtaa gctgtgtcaa gcgcccacac atcgctatga accgcaacac cttctcttcc
     1321 gtccgtgacc cctcctgcaa tatacatttt ccagttatct gatgaaaaag agaacgaatc
     1381 tcttgaaaca ggatattcat cagattctat ttcaatgaaa gtatctccgc taagtatgta
     1441 aggttcataa tattgtccgt tgttacggag cataacaagt tcccttccgg cacttaagat
     1501 atttatctcg ttttccggat aaggcatctg cattatttct ctgaatctat aggtcatgcc
     1561 gctgccgatg tttgtctgct gtccgctgtc gtccttttca agagaataga gcatattcgc
     1621 acttgtaagg aaaattctcc cttttgcaac agcacctttt cctttgatca gatcttcctg
     1681 cggtttgggt gcaattcctg caagaattat ttcgcttgga ttatcttcat cattgattcc
     1741 gaaatatatt tcattttcgc cttcaaacac cgatataatg ctcccttcat cattgaatgt
     1801 cgcaataatg ttgtcagcat tgttgagttt tcttttttca cttttcagaa tattatcctt
     1861 atatatccag agttcagtca cagatgatac tgcatctgaa aattttacac atcctgaaca
     1921 tgttgaattt gatctttccg agctgtttaa catttcaggg gtaattttga acgggaaaaa
     1981 gttctgtctc agattttcag tgtaataact aagttttgtt ccctgcttgt tctctctttt
     2041 tgtccttgcg tttatataca acacattcct gaaacagttc ggatttataa attcttcttc
     2101 ctcatcataa taatcttcat ctgcactgca attttccgtt cctgccgatc caactgaagc
     2161 agcataatta atgaacggct cctttaaatt ctgctgacct gatgaaacat aagtcgtgta
     2221 aagtctttca taatcttttt gtaaattctt tgtccagtcc catttttcaa ttgccgattt
     2281 gctgtccttt gttttgttta ctacactcaa ttttcccata aatgagctta ttttaggggt
     2341 ctgccatgcc agtttattaa gctgtctatt ataaggttca ggatcgcccc cgtgactgta
     2401 tccgaaactc gaatcattta tcgtcggata acctttccca agatccgatg ttgtgcagta
     2461 accgcctttg ccccactcat ccattaattc cagatccatc cagcagtatt ttgcagttga
     2521 tccgggggtt gtgtcaccga taactttgta attcagatca acgaacctgt tcacctccga
     2581 tttacctctg aaaaaaagcg gatttgaaga tgtcgttgaa atcagtttcg taactgaaga
     2641 atgatccgtg tcacacgcat cgcctgtgcc gtcaagatca tgatccggct gccagtacca
     2701 gacctttcta cataaccact ggttccccat aaagttcact atacacttgt tgccaaagta
     2761 tccgtcattt tttctctcct gatttttcga cgctcctcca ctgaaagaaa gtttcagcaa
     2821 acattcatac cagttatcat catcatcgca ttcctgagtt ttcgcagaaa taatttcgtc
     2881 attcaatgat gctactacaa atttgttttt aacatttgga caattgtcgc acccgttggg
     2941 gatcccatct ttatcatcat cctctcttgg attgtatatt gaaatgctac ccatcatcgg
     3001 acacggatca cagacatcac caacaccgtc ttcatcataa tcagcctgat cggggttata
     3061 aatattaggg caattgtcat cagaattatc aaatccatca ttgtcatcat ccattccata
     3121 ttgcagacaa gtcattaatt ttaccgaagc ttctacggca tcaatataaa attcaacatt
     3181 gtcttctgtc gtgttttcat atcctcctgc ttttcctttt gatttatctc ttacagaatc
     3241 gaacaggtca taaacaccac tttctaaata atttccttct tcgtcatata ttcccagtcg
     3301 ctgacatatc tctttgttag aaaaaatatt gggagcaatt ccaaaaagtg catcaagtct
     3361 ttttttcagc atatctggtc caccatattt gccaaaatct ccatttttca acatattatc
     3421 aaatgttttt tctctccatt gatgcatttc tcctgaccag tttattgcat ggtaaggcat
     3481 cgcaagatcc tgtataaaat gaagtgccca tccaagatat atcatcgcta ctctattgct
     3541 tttagaaggg aaaggattcg taagctcttc gatgaattca tcaatattat tttcatcata
     3601 attgtccggc atccatattg gccatgacgg ggttccactt gcacataaat ctctgtcaga
     3661 gcaattagta cttgtcggat cataacttct attcggctta caaataaacg gttgtccccc
     3721 aagccatgtc gagggcaaag aagcccacct tctgtcaaca ctccacattt tccatgtgtc
     3781 cataattgca ctaccggttg caggaacaat atcgtcatct aaacaattgg aagcaagtac
     3841 catatcatta aatgaaggag gctcgttcac attcgcttcc caaaactttc ttgccaaacc
     3901 aaaaagtatc gttccatatt ttgcagcacc aatttcagaa tttacaaaaa cccatgcccc
     3961 atctccaatt ccagtattcc caactgcatt ttcctgtcgc aatataacaa ctccgttacc
     4021 tcctatttcc ttgtttgaaa aatgtaaata attatccgcc gcataatctc catcaaaatg
     4081 atttttacct cccacttcaa gaaaattata aattcccatg taataattag gtctttttga
     4141 gccggcttct ctccttaaga atcgcacttc aactcttcca ttatctgttt cgcttatttt
     4201 atattcatct gatgaataaa tatttcccaa aaatatttcc ggatcaatat tgctcaaaac
     4261 acctgaattt cttccatatg gattttcagg tcttccaagc caaggctcgt cagcaaaagc
     4321 cgcaccatat aacaaataaa tcaacttgtc ttccattaaa ttaccaggat ctgataagaa
     4381 ataaggaaaa ataactcctc gcttttctat ttcccaataa ctttcccttg ttattgcctg
     4441 atgaccattg taattcccaa aatattcaca agttttgttg tcatctaagc aataggaatg
     4501 aaaatcttcg ttccatccat aaatttcaaa aaatatcaaa ctccaaataa ttgcaaaaag
     4561 tactctcctc atctcacccc tccatcaaaa aattaataaa actatccctt gaaaaacgct
     4621 tgaaatattc cgaatgtatc tgaaaaaatt aaagaattga cttgtctctt gtctcttgtc
     4681 tcttgtctct tgtctcttgt ctcttgtctc ttgtc
//
LOCUS       DAOOYB010000228         4667 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_21464_length_4667_cov_9.527537, whole genome shotgun sequence.
ACCESSION   DAOOYB010000228 DAOOYB010000000
VERSION     DAOOYB010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4667
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21464_length_4667_cov_9.527537"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(225..1385)
                     /locus_tag="PL056_11170"
     CDS             complement(225..1385)
                     /locus_tag="PL056_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47619.1"
                     /translation="MKKIIVLMIFLFAMFPVIAVDDDDVAPCQGSWNLDVLFTEVYWK
                     ENSTWYKIPDNKMSSIYPWVCKDHGGCPGGQLPWGDPFTCYISGNAYYKVTVNASQVP
                     CVNDYPYVQSCWVWRNIKLGSSIDIYRVVIPNVNHNTIEPWLNYYSLPNYSMYAREES
                     VNDAIWSSNNSGPPYYISIYSSESRWDLRNGNDNWVKTDPGWGVTTRVKKLEKRYRLF
                     FNGSYIYLWMSTSNMTAFKNFEIHLQKGTSPTDSMHFSKNYESYNDNQNDFIRNIDYD
                     MAQRIKHNSINEILNEILMSSSFEEDFSTSIRIDNFSEGYIAGLHIEKIDKNLMDKQN
                     SIFEGATILSINGYKLSEFELIENVFEYLEKNKILSIVLLDRNMMLYEMVEE"
     gene            complement(1700..2770)
                     /locus_tag="PL056_11175"
     CDS             complement(1700..2770)
                     /locus_tag="PL056_11175"
                     /inference="COORDINATES: protein motif:HMM:NF023462.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3524 domain-containing protein"
                     /protein_id="HPM47620.1"
                     /translation="MKAFLEPYYGGSHKSFADSVIKRFPDEFMLFTLPPRHWKWRMHG
                     SAVTFAEQVIATAIEFSEIFVTSLTDLALLKSLLGSRLPKCRYTIYFHENQLSYPWSD
                     IDKGPEERKDMHYGFLNFTSALTADRVVFNSEFNKDSFFNNLTPFLRSFPDCRLDKRI
                     DEILKKSEVIYPGIEPVPEFERIGNEVAVILWNHRWEYDKNPDEFFLALFKLKEKGIE
                     FKLAVAGEEKVRAPEIFGVAKEKLKNEIISFGYLENREQYIKLLKKSDIIPVTSNHEF
                     FGISVLEAASAGVIPLLPKRLSYPELFPLENFSHLYYENGQLAEYIEKTILKINRFDR
                     TQIKKRADLFNNSQSLSAFLKF"
     gene            2888..4522
                     /locus_tag="PL056_11180"
     CDS             2888..4522
                     /locus_tag="PL056_11180"
                     /inference="COORDINATES: protein motif:HMM:NF015541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004806 - triglyceride lipase activity
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HPM47621.1"
                     /translation="MVSFEKIVKVCFSVFIVLSFVSCSGDKKTDVTDEDNTNSNDADQ
                     TIVPDETADEAVDESVDKDLSDEVLTESETVDETADETIIDEEIDEEADEDTFVPYDP
                     RVAPALCGINEYTWLKQEDLQMGTGLGFILESKELLTDLEGIAARKKTALITLMETND
                     EVKSRNIVRRPKHDVRIFKIRYQTQDKGKLVDATGIVAFPDLKDNTETFPVVSILHGT
                     APGFADACAPSTDETAEGEATGTGVLLASFGYIIIAPDYIGLKSMGEASTEIHPYLIA
                     EPTAISVLDMMRAARNLLSLINAKAIPGDNAIIGGSQGGHAAAFTTRYQPYYAPELVI
                     KGASWGIPPTDLGTHAQQALQNFVKASGNTALFLLTSNSWYTGDHTNLSEVFVSPFDV
                     DFPVAAVDPGCDMGDSVDGMDSLDDLYSTEILAEAAVEPFLVNMPPWDCFVEVNTLPT
                     SPLPRIDDIPALMVLGELDDLVDPGIETASFQKLCEQGYQINYKMCQGAGHVDGWAWS
                     LEEQFDWVDARIANVPMTDYCTVKEPIRCANTPDGE"
BASE COUNT         1423 a          939 c          845 g         1460 t
ORIGIN      
        1 tacaacaggt gcaatatgcc aattctcttc aacatcttca tctggaattt catcattatc
       61 tttctgctct tcagattgat cagtatctaa aatatcatta cccagaatat tttctttttt
      121 ttcacatgca aaaacaaaaa cactcaaaaa aaacagaagc aaaatcttct tcataaactt
      181 ctccatatat gggaggaagt gaaaccaccc cctccctcaa aaaattattc ttcaaccatt
      241 tcgtaaagca tcatatttct gtctaaaaga acaattgaaa ggattttgtt cttttctaag
      301 tattcaaaaa cattttcaat taactcaaat tcactcaatt tataaccatt tatagacaaa
      361 atcgtcgctc cttcaaatat tgagttttgt ttatccatta agttcttgtc tattttctca
      421 atatgaagtc cagcaatata accctctgaa aaattatcaa ttcttattga tgtagaaaaa
      481 tcttcctcaa atgaagacga cattaaaatt tcgtttaata tttcattgat tgaattatgc
      541 tttattcttt gagccatatc ataatcaatg tttcttataa aatcattctg gttgtcatta
      601 taactctcgt aatttttgct aaaatgcatt gaatcagttg ggcttgtacc tttttggaga
      661 tgaatttcaa aatttttgaa cgctgtcata ttgcttgtac tcatccaaag ataaatatag
      721 cttccattaa aaaagagtct gtaccgtttc tcaagctttt taactcttgt tgttaccccc
      781 catccagggt cagtttttac ccaattatca tttccattcc tcaaatccca tcttgattca
      841 gatgaatata ttgaaatata atatggaggt ccactattat tagagctcca tatagcatca
      901 tttacgcttt cctccctagc gtacattgaa taattaggta atgagtaata atttaaccac
      961 ggctcaatgg tgttgtgatt aacattggga attacaacgc gatatatatc aatagagctt
     1021 ccaagcttta tatttctcca tacccaacat gattgaacat aaggataatc gttcacacat
     1081 ggaacttgag atgcgttaac agttacttta taataagcat ttccacttat ataacatgta
     1141 aagggatcgc cccatggaag ctgtcctcct ggacatcccc catgatcttt gcatacccaa
     1201 ggataaatag aactcatttt attgtctgga attttatacc atgtagaatt ttctttccag
     1261 taaacttcgg taaacaaaac atccaaattc caagaccctt gacatggagc aacatcatca
     1321 tcgtcaacag ctattactgg aaacatagca aaaagaaata tcattaaaac tattatcttt
     1381 ttcatgtttt tctccaaaat tttcgttaaa aaaacatctt ttcaaaattt aatgaaccgt
     1441 tttcccaaca actttttaag gggggacccc tgaccggaat ttttgtaaga aagagtgatg
     1501 aatgaatgaa tgagcaataa ctgtgccaga attgccttta aactcaaggt tttcatcact
     1561 ttcttcattt tttttaaatt aacaacacgg cttatgctaa cagcgactaa gtttcatgtc
     1621 aaattatttt caatccataa tgtattcaaa cttataagtc ttaatttaag aacaatgatt
     1681 cctgatgttt gcaaaaactt cagaatttca ggaaagctga taacgactgt gagttattga
     1741 aaagatcggc tctttttttg atctgtgttc tatcgaatct gtttatttta agaattgtct
     1801 tttcaatgta ttcggcaagt tgtccatttt cataatacag atggctgaaa ttttctaacg
     1861 ggaaaagttc ggggtagctg agccgttttg gaagaagagg tatgactcct gctgatgcgg
     1921 cttcgaggac acttattccg aaaaactcgt ggtttgatgt gactgggatg atgtcacttt
     1981 ttttcagaag tttgatgtat tgttctctgt tctcaagata accgaacgat attatttcat
     2041 ttttaagttt ctcttttgca acaccgaata tttcaggagc tcgcactttt tcttctccgg
     2101 caactgcaag tttaaactca attccttttt cctttaattt aaaaagagcc agaaaaaatt
     2161 catcggggtt tttgtcatat tcccagcggt gattccagag tatcacagcc acttcatttc
     2221 caatccgttc aaattcagga accggttcaa ttccgggata gatcacttca gattttttta
     2281 atatttcatc tattctttta tcaagccggc aatcgggaaa tgatcttaaa aaaggagtta
     2341 agttattgaa aaaactgtct ttattaaact ccgaattaaa cacaacccta tcggcagtca
     2401 gtgctgaagt gaagtttaaa aacccgtagt gcatgtcttt tctttcttcg ggacctttat
     2461 caatatctga ccagggatat gaaagctggt tttcgtgaaa atagatcgtg tatcggcatt
     2521 ttggcagtct tgaaccaaga agtgatttca aaagggccag atctgtcagt gatgtcacaa
     2581 atatctctga aaactctatg gctgttgcaa taacttgttc agcaaatgtt accgcagaac
     2641 cgtgcatcct ccatttccag tgtcttggag gaagcgtgaa aagcatgaat tcatcaggga
     2701 atcttttaat aactgaatcg gcgaaacttt tgtggcttcc gccgtaatat ggctcaagaa
     2761 aagctttcat aaagcccata ttaccgaaat ttgtatgtta ttcaacaata tcgccttgac
     2821 gaaaaagtca ttttatggat aattgcgact cgtgtgtcga gtattaaagt taagtgaggg
     2881 ttaagaaatg gtaagttttg aaaaaatcgt aaaagtgtgt ttttctgttt ttattgtttt
     2941 atcttttgtt tcctgttcag gtgacaagaa aactgatgtc accgatgaag acaacacaaa
     3001 ttcaaatgat gcagatcaga ctattgtacc tgatgaaact gctgacgaag cagttgatga
     3061 atctgtcgac aaggacttgt ccgacgaagt tttaacggag tcggaaacag ttgatgaaac
     3121 tgctgacgaa acaattattg acgaagaaat tgacgaagaa gccgatgaag atacttttgt
     3181 cccttatgat ccgagagtcg cccctgcact ttgcggaatt aatgagtata cctggcttaa
     3241 acaggaagat cttcaaatgg ggacaggact tggatttatt cttgaaagca aagaactcct
     3301 gaccgacctt gaaggaattg ccgcaagaaa aaagactgca ctgataacat tgatggaaac
     3361 aaatgacgaa gttaaaagca gaaatattgt cagacgaccg aaacatgatg tcaggatatt
     3421 caaaataaga tatcagactc aggacaaagg aaaacttgtt gacgccacag gaatcgttgc
     3481 atttcccgac cttaaagaca acacggaaac ttttccagtt gtgagcattc ttcacggaac
     3541 tgcacccggt ttcgctgatg catgtgcccc gtcaactgat gaaactgccg aaggtgaagc
     3601 aaccggaacc ggcgttcttc ttgcatcttt cggatacata atcattgctc ctgactatat
     3661 cgggcttaaa agtatgggag aagcatcaac agaaattcat ccttatctga tcgctgaacc
     3721 gactgccatt tcggttcttg acatgatgag agcggcaagg aacctccttt cgcttatcaa
     3781 tgcgaaggca atacccggtg acaatgcgat aatcggcggc tctcagggtg gacacgctgc
     3841 ggcatttaca acaagatatc agccatatta cgctcctgaa cttgttataa aaggtgcatc
     3901 atggggaatt ccgccgacgg acctcgggac acatgctcag caagcgttgc agaattttgt
     3961 aaaagcaagc ggaaatactg ctctttttct tctcacctca aattcatggt acactggaga
     4021 tcacacaaat ctttccgaag ttttcgtatc cccttttgat gtggacttcc ctgttgcggc
     4081 agttgatccc ggatgtgaca tgggcgactc agttgacgga atggattctc ttgatgacct
     4141 ctattcaact gaaatacttg cagaagccgc cgttgagcca ttccttgtca atatgccgcc
     4201 gtgggactgt ttcgttgaag ttaacactct gccgacatca ccccttccaa gaattgatga
     4261 cattccggca ctcatggtgc ttggcgaact tgatgacctc gtagatcccg gcattgaaac
     4321 tgcatctttc cagaaactct gcgagcaggg atatcagatc aattataaaa tgtgtcaggg
     4381 cgcaggacat gttgacggct gggcatggtc acttgaagaa cagtttgact gggttgacgc
     4441 aagaatcgca aatgtaccca tgacagatta ctgcaccgtc aaagaaccca tcagatgcgc
     4501 caacacccct gacggcgagt aggttctctc aaatagcaaa cttcacgcct tgatgtttct
     4561 tcagttcttc aaaaagcata tacattatat cttttgaggc gatatagact ttcccggtgt
     4621 aacacatata gtttccggcg ttactttttg aaatgatcca ctctttt
//
LOCUS       DAOOYB010000229         4665 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_21475_length_4665_cov_11.694143, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000229 DAOOYB010000000
VERSION     DAOOYB010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4665
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21475_length_4665_cov_11.694143"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            84..1307
                     /locus_tag="PL056_11185"
     CDS             84..1307
                     /locus_tag="PL056_11185"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HPM47622.1"
                     /translation="MENKKSVAILCSGGPAPGINTVVCSIAKVFLKDNYRVIGLNYGT
                     KTLFTDNPDTVEIDFEMADQYFNRGGSFLKMSRYKPKDSEFNPDFFINNNVELLVTVG
                     GDDTASTANRITKYLNRQDLPVRNIHVPKTIDNDLPLPDRLPTFGYHSAKNEGVRIAT
                     TVYEDARTSGNWFVVSSMGREAGHLAFGMGSACHYPMIIIPEMFKNAEVTFDRMIKMM
                     ISSIVKRKILGMSYGAIMISEGVFHFMSDEEMINSGIKFSFDEHGHPELGKVSKAHIF
                     NVLLSEKLDELGIKIKCRPVEIGYEVRCCPPTAFDLKYCTLLGFGVKKLFDEGKSGCI
                     VVCHNDGRVLPLYLEDVEDPKTGKIKPRLVDTEAQDFKMFVENFHYIKEDDYQKASKY
                     LKNPQLYDLKNILGW"
     gene            1397..>2907
                     /locus_tag="PL056_11190"
     CDS             1397..>2907
                     /locus_tag="PL056_11190"
                     /inference="COORDINATES: protein motif:HMM:NF036933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgGFc-binding protein"
                     /protein_id="HPM47623.1"
                     /translation="MRKFFIVMQMIFATVVLFSCSQSNDEESTKCESKLDCPFGQECV
                     DGECVKPGEGGNDNETVDDAPDTTPCDPGDEIDCYEGPDGTDGIGICKIGKKKCNDNG
                     IGYGECVGQVLPEKDKCTDNLDNDCNGVINDGYESGAEGCLCKPKTSECLDGENIIIC
                     NEDGTAFTDAKCDPLQGLTCSNGACDGDCSAGKIGINYIGCDYYPTVLPNSSQLDFVN
                     FKYAVAVSNSNSSEATVTVTQNDNVIVTESVPGNSMKIIFLNWTSNSSSTMTSIKQNE
                     AYRLRSTKPVTLYQFNPLDYQSGGSYSYSNDASILMPTNAWNKEYMVASRASIEDGPG
                     YYVVVAAHDNTTVTTTPSPTGNKDIFAGAGINMDGTGTVVLNEGDVLHVASAGKMDGL
                     SVDISGTIISADKPVQVFGGHNCTYVPYDQTAACDHLEESMFPIATLGSDYIVSAPWI
                     KLNTVKAVMTRIFAVDEGDTTLTFDPPNAGWPATIFGRGSFVEIDSDMAFKISA"
     assembly_gap    2908..3007
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3072..4088)
                     /locus_tag="PL056_11195"
     CDS             complement(3072..4088)
                     /locus_tag="PL056_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HPM47624.1"
                     /translation="MGSNHTKFSDSQIIIYEATDGKIKIDVRVEDETVWLTQKMMAAL
                     FDCSIDNVSLHLRNIFKESELLENSVVEEFSTTASDGKNYKTKHYNLDAIIALGYRVN
                     SKRATQFRIWATQRLKEYIVKGFALNDERFKSGNSMNYFNELQERIREIRLSERFFYQ
                     KIKDIYKTSIDYDPQNEKTVEFFKIVQNKLLWAISEQTAAELVYRRVDASLPLLGMQS
                     FDKTAAVSIKKSDVSIAKNFLKEKEIKLLGIIVEQYLAFAESMAQQQIPMYMKDWIER
                     LDSIIKMNGKELLTHAGKISHESALEKAGQEYEKYLDEKRKKELEESLKELENDIKKL
                     TSEV"
     gene            4247..>4665
                     /locus_tag="PL056_11200"
     CDS             4247..>4665
                     /locus_tag="PL056_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47625.1"
                     /translation="MKVAILIFTFFLISCNSNDPKTDADKAVPDKDSVAVIDSDTQDA
                     DIPVSDPDIVKNDVDNDLVKPDNDSAGDKDIISDEVPDPDTAEEWPDYDYPVGKEGDP
                     DCPSLLNAGFPYIDNDGKKHFCRKCDLPAPANDPQCI"
BASE COUNT         1344 a          836 c          976 g         1409 t
ORIGIN      
        1 cacataattt ttgataattt ttctgtttag gtttatcata cctgcggttt tgtaagatgt
       61 tttttcataa atggagcaag aaaatggaaa acaagaagtc tgttgcaatt ttgtgttcag
      121 gtggtcccgc ccccggtatc aacactgtcg tatgttcgat cgctaaggtt tttctgaagg
      181 ataattacag ggttataggt cttaattacg ggacgaaaac cctttttaca gacaatcctg
      241 acacagttga gatcgatttt gagatggctg atcagtattt taacaggggc ggatcatttc
      301 tcaaaatgag cagatacaaa ccgaaagatt ctgaatttaa ccccgatttt ttcataaata
      361 acaatgttga acttcttgtt actgtaggcg gagacgatac tgcttcgact gcaaacagga
      421 taacaaaata tctgaacaga caggatctgc cggtcagaaa tatccatgtt ccgaaaacga
      481 ttgacaacga tcttcctctg cccgaccggc tcccgacatt cggttatcat tctgcaaaaa
      541 atgaaggtgt caggatcgcg acaactgttt atgaagatgc aagaacaagc ggaaactggt
      601 ttgtcgtttc atcaatggga agagaagctg gacatcttgc tttcggaatg ggttcagcgt
      661 gtcactatcc gatgataata attcctgaaa tgttcaaaaa tgcggaagtg acatttgata
      721 gaatgataaa aatgatgatc tcttcaatcg ttaaaagaaa aattctggga atgagctacg
      781 gcgcaataat gataagcgaa ggtgttttcc acttcatgtc agatgaagag atgatcaatt
      841 ccgggataaa gttctctttt gatgagcacg gacatccgga actcggcaaa gtttccaagg
      901 cccacatttt caatgtcctt ttaagtgaaa aacttgatga actcggaata aagattaagt
      961 gcagacctgt tgaaattggc tatgaagtga gatgttgtcc tccgacggca tttgatctta
     1021 agtattgcac attgctggga tttggcgtca agaaactttt tgatgaaggg aagtcgggct
     1081 gtatagttgt ctgccataat gacggtcgtg ttcttccact ttatcttgaa gatgtcgaag
     1141 atcccaagac tggaaagatc aaaccgcgac ttgtagatac tgaggcgcag gattttaaaa
     1201 tgttcgttga aaactttcac tatattaagg aagatgatta tcagaaagca tcaaaatatc
     1261 ttaaaaatcc gcaactttac gatcttaaga atattctcgg ctggtaattt ttcttaaatc
     1321 tctttattta gttgaaaaaa gaatgttatg atatattatt acaccgttgt gtgtaaaaaa
     1381 tgatggggga tggataatga gaaaattttt tattgttatg caaatgattt ttgcaacggt
     1441 ggttttattt tcatgcagtc agagtaatga tgaagaaagt acaaaatgcg aaagcaagct
     1501 tgactgccct ttcggtcagg aatgtgttga cggtgagtgc gtaaagcccg gagaaggcgg
     1561 taatgataat gaaacagtgg atgatgcgcc cgacacaaca ccctgtgatc cgggagatga
     1621 aatagattgt tacgaaggtc ctgacggaac tgacggaata ggaatctgta aaattgggaa
     1681 aaagaaatgt aatgacaacg ggatcggata cggtgaatgt gttggacagg tgcttcccga
     1741 aaaagataag tgtactgaca atcttgataa tgactgtaac ggagttatca atgatggtta
     1801 cgaatcaggg gctgaaggat gtctttgcaa gccgaaaact tctgaatgtc tggatggcga
     1861 gaacattata atatgcaatg aggatggaac tgctttcact gatgcgaaat gtgatcctct
     1921 tcagggattg acatgcagta acggtgcgtg tgacggtgac tgttcagcag gaaagatcgg
     1981 gataaattac attggatgcg attactatcc gacagttctt ccaaacagct cacagcttga
     2041 ttttgtcaac tttaagtatg cggttgcagt atcaaattca aacagttctg aagcaacagt
     2101 gacagttacc cagaatgata atgtcattgt tactgaatcg gttcccggca attcaatgaa
     2161 aatcatcttt cttaactgga cttcaaattc aagcagcaca atgacaagca ttaagcagaa
     2221 cgaagcatac cggctcaggt caacaaagcc ggttacttta tatcagttca atccgcttga
     2281 ttatcagagc ggcggcagtt attcgtattc aaatgatgcg tcgattctta tgccgacaaa
     2341 tgcatggaat aaagaataca tggttgcttc ccgtgcctct atcgaagatg gtccgggtta
     2401 ctatgttgtt gttgccgctc atgataatac gactgtgacc acaactcctt ccccgaccgg
     2461 aaacaaggat atttttgcag gggcagggat caatatggat ggaacgggca ctgttgttct
     2521 gaacgaagga gatgtgcttc atgttgcttc ggctgggaaa atggacggtc tttcagttga
     2581 tatttcaggt acgatcatca gtgcagataa gccggtacag gttttcggcg gacacaactg
     2641 cacttatgtc ccatacgatc agactgcggc atgtgatcac cttgaagaat cgatgttccc
     2701 tattgcaact ctcggaagtg attacatagt ttcagctcca tggataaagc tcaacacagt
     2761 taaagctgtc atgacaagaa tatttgcagt cgatgaaggt gatacaactc tcacatttga
     2821 tccgccgaat gccgggtggc ctgcaacaat attcggcaga ggaagttttg ttgaaattga
     2881 cagcgatatg gcttttaaaa tttctgcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnttc ttcatcaatg tctacggcta cggtgattat acaagctact ggtatcccgg
     3061 cggacttaac ctcatacctc agaagtaagt tttttaatgt cattttcaag ctctttgaga
     3121 ctttcttcca gctctttttt tcttttttca tcgagatatt tttcatactc ctggccagct
     3181 ttttcaagag ctgattcatg agatattttt cctgcatgtg tcagaagttc cttgccattc
     3241 attttaatta ttgagtcaag ccgttcaatc cagtctttca tgtacatcgg aatctgttgt
     3301 tgtgccattg attctgcaaa agcaagatat tgttcaacaa tgataccaag aagtttaatt
     3361 tctttttctt taagaaagtt ctttgcgata ctcacatcac tcttttttat tgaaacagcg
     3421 gcagttttat caaaagactg cattccaaga agaggcagag atgcatcaac tctgcggtaa
     3481 acaagttctg ccgcagtctg ttcacttatt gcccagagga gtttattttg aactatttta
     3541 aaaaattcaa ccgtcttttc gttttgggga tcgtaatcaa tgcttgtttt gtagatgtct
     3601 tttattttct gataaaaaaa cctctccgaa aggcgaatct cacggattct ttcctgcaat
     3661 tcattgaaat aattcatcga atttccagat ttgaaccgtt catcatttaa agcaaaccct
     3721 ttgacaatat attctttcaa cctttgtgtc gcccagattc gaaattgagt ggctcttttg
     3781 ctgtttacac gataaccaag agcaattatg gcatcaaggt tgtagtgttt tgttttatag
     3841 ttttttccat ctgaagcagt tgtcgagaat tcctcgacaa ctgaattttc taataattct
     3901 gattccttga aaatgtttct caaatgcaac gaaacattat ctattgaaca gtcaaagagt
     3961 gcagccatca ttttctgtgt caaccagacg gtttcatctt cgacacgaac atcaatcttt
     4021 atttttccgt cagtagcttc ataaattata atctgcgaat cgctgaactt tgtgtgattt
     4081 gatcccattt ttcggtttcc tccaccaatt ttaaatcccg atgataataa catgttagac
     4141 agaagttgta aaattttgag gatttcaagt aagtgcttgt gctatttgac taaaataggt
     4201 ttttgagata atctaatccg ttaattattt gaatttcaag ggttttatga aggttgcgat
     4261 tctgattttc acattttttc tgatttcatg taattcaaat gaccctaaaa cggatgcgga
     4321 taaagctgtt cctgacaaag attcagttgc tgtcattgat tctgacactc aggatgctga
     4381 tataccggtt tctgatcctg atattgtcaa aaatgatgtg gataacgatt tggtaaagcc
     4441 ggataatgac agtgccggag ataaggatat catatctgat gaagttccgg accccgacac
     4501 tgcagaagag tggccggatt atgattatcc tgtaggtaaa gagggagatc ccgactgtcc
     4561 gagtctgctc aatgcggggt ttccatatat tgataatgac ggaaagaaac atttctgtcg
     4621 aaaatgtgat cttcctgcac ctgcaaacga tccacagtgt ataag
//
LOCUS       DAOOYB010000230         4654 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_21542_length_4654_cov_11.186127, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000230 DAOOYB010000000
VERSION     DAOOYB010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4654
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21542_length_4654_cov_11.186127"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            276..1745
                     /gene="guaB"
                     /locus_tag="PL056_11205"
     CDS             276..1745
                     /gene="guaB"
                     /locus_tag="PL056_11205"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HPM47626.1"
                     /translation="MENDTKKIEMALTFDDVLLVPHYSEVLPSQVDISSKLTSKITLN
                     TPLMSAAMDTVTEVDTAIAMARQGGMGVIHKNMTIEEQSKMVKMVKRSEAGVITNPIT
                     ISPNAHISDALELMQSNRISGIPVTEDEKLVGIITNRDLRFETDMNKKVGELMTRKLI
                     TVKENFPMVEAKKLMHEHRIEKLLVVDANGGLAGLITTRDIENQEKFPNSAKDNLGRL
                     VVGAAISPSVSGMERAAALVKAGVDALVLDSAHGHSKNVIDTVAELKSQFPDVDIIAG
                     NIVTAEAAEALFQAGADVVKVGIGPGSICTTRIIAGVGYPQLSAIFNVAPVARKWGRA
                     LIADGGIKYSGDVVKAIAAGADAVMIGGMFAGTAEAPGETVLYQGRTYKMYRGMGSLG
                     AMKKGSKDRYFQDNVVDEQKFVPEGIEGRVAYKGPLSDTIYQLSGGLKAGFGYLGALN
                     VESLRKNVKFIQITNAGYRESHVHDVTITKEAPNYRTER"
     gene            1888..2154
                     /gene="rpsO"
                     /locus_tag="PL056_11210"
     CDS             1888..2154
                     /gene="rpsO"
                     /locus_tag="PL056_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005838271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HPM47627.1"
                     /translation="MFDKVRKTEVISKFGINEKDTGSPEVQVALLTERINMLTDHFKE
                     HKKDNHSRLGLLKMVGKRMKLLKYIKGKDFGTYNTLIKELGLRK"
     gene            2234..4462
                     /gene="pnp"
                     /locus_tag="PL056_11215"
     CDS             2234..4462
                     /gene="pnp"
                     /locus_tag="PL056_11215"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HPM47628.1"
                     /translation="MKEIKESIVFNGKDLSIATGRYAKQADGAIWLTYGGTVLLATVV
                     ASKEEVSDDIDFFPLTVDYVEKFYAAGRFPGGFLKRESQQSTAEKLTSRLIDRPLRPM
                     FADWFKNETQILVNLLSYDSECDPKVISILASSAALMVSDIPFNGPVAGCRIVKKNGE
                     FIINPSLNGEPEKYEIDITLAGNRESIMMVEGEALEASEDDMTAAIEIGHKAIQPLLD
                     IQEKLAKKISPEKRAEKEIKIDEKLKKFVEKNAASLVKKALSTTVKLERYSLVKEAKE
                     KVVELVIKALEDGEKFNDADNQEKVVKRSKGFFENYLKESMREMIVKKNKRIDGRDLT
                     SIRPITIDLGVLPKVHGSAVFTRGETQSLGTITLGIGHDEQRVDTVTEEDSKNFMLHY
                     NFPGFSVGEVARLKSPGRRELGHGNLAERALSPIIPSKDEFPYTIRVVSEILESNGSS
                     SQATVCSGSLALMDAGIPFKKHVAGIAMGLIKEKNDFFILSDILGDEDHLGDMDFKVA
                     GTKDGITAIQMDIKIEGLPKDVMERAMIQAKVGRMHIIGEMDKAITGPRSELNPNAPK
                     LLKMKIEVEKIKDLIGTGGKNIRSIVEQTGADIEVEQDGTVKIAAKDQQILDLTIALV
                     KSFTDSVELGKIYEGEVTRIEEYGAFVEIMKGTTGLLHVSKIADERVENVTDYVKLGD
                     KVKVIVTEIEFGGKFKLSMKPTDFEKDWKKEKENRPPKRDDRKGGHDRNDRGRDRDNR
                     RR"
     gene            4443..>4654
                     /locus_tag="PL056_11220"
     CDS             4443..>4654
                     /locus_tag="PL056_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HPM47629.1"
                     /translation="MTTADADILIKFKLENGAVLPKYETEHSSGMDVRSVENCLIDPN
                     SWKLISTGLFPEIPVGYEIQVRSRSG"
BASE COUNT         1566 a          844 c         1097 g         1147 t
ORIGIN      
        1 aaggaaaatg gatggtttac cagttcagag ctgttgcatt acaagcaata agagatcttg
       61 actgctattg cgaaaaagaa ggggtttgtc cgtttgagca accatgagca ggtcagccat
      121 gaacatactg atgatccaaa aaagatttaa acaattcaat atattcaatt agaaatcaat
      181 ttaaaagatt ttggctcatg ccctgattgc ttgacatcgt ttcattagac attagaatag
      241 caccaattta ttgtttattt acagagagga taacaatgga aaatgataca aaaaaaatag
      301 aaatggcgct tacttttgac gatgttcttc ttgttcccca ttattcggaa gttcttccaa
      361 gtcaggttga catatcttca aagcttactt caaagataac tctgaacact cctttgatga
      421 gcgcggcgat ggatacggtc actgaagttg atacagccat tgcgatggca agacagggcg
      481 ggatgggcgt cattcacaag aacatgacga tcgaagaaca gtcaaaaatg gtcaagatgg
      541 tcaaaaggtc ggaagccgga gtgataacaa atccgataac catatctccc aatgcacaca
      601 taagcgacgc ccttgagttg atgcagagca accggatctc ggggattcca gtcacggaag
      661 atgaaaaact tgtcggcata atcaccaaca gagatctccg ttttgaaacc gatatgaaca
      721 aaaaggtcgg cgaactgatg acaagaaagc tgatcacagt aaaagagaat ttcccgatgg
      781 ttgaagcaaa aaaactcatg cacgaacaca ggatagaaaa gcttcttgtt gtagatgcaa
      841 acggcggtct ggcaggactt ataactacaa gagacataga aaatcaggaa aaatttccaa
      901 attctgcaaa agacaatctg ggccggcttg ttgtcggagc ggcgataagc ccatcggttt
      961 ccggaatgga aagggctgcg gcgcttgtca aagcaggtgt cgatgcactt gtccttgact
     1021 cggcacacgg acactcgaaa aatgtgatag atacggtagc tgaactcaaa agccagtttc
     1081 cggatgttga tataatcgca ggtaacattg taacggctga ggcggctgaa gcacttttcc
     1141 aggcaggggc ggatgtcgtt aaagtcggta taggtcccgg atcgatatgt acgacaagaa
     1201 taatcgcagg tgtcggatat cctcagcttt ccgcgatatt caatgtcgca ccggttgcaa
     1261 gaaaatgggg cagggctctc attgcggatg gaggaataaa gtattccgga gatgttgtca
     1321 aagccattgc tgccggagca gacgctgtaa tgatcggcgg aatgttcgca ggaaccgctg
     1381 aagctccggg cgaaacagtc ctttatcagg gaagaaccta taagatgtat agaggaatgg
     1441 gttctctggg tgcaatgaaa aaagggagca aggacagata ttttcaggat aatgtggtcg
     1501 atgagcagaa atttgttcct gaaggtatcg aagggcgcgt tgcatacaaa ggtcctcttt
     1561 ctgacacaat ttaccagttg tccggcgggt tgaaagcagg attcggatat ctgggggcgc
     1621 tgaatgttga atctctcaga aaaaatgtaa agtttatcca gataaccaat gcaggatacc
     1681 gtgaaagcca tgttcatgat gtcacgataa cgaaagaagc tccgaattac cgtacagaaa
     1741 ggtaaaaaga acggttttgt aatatcttga aattgttaat taaaaatgtt tcacaatatt
     1801 cctgctaaat aaaataattg acttgggcac catcacaaat ataatgccgt aagttttttg
     1861 tatcgttcaa aaaaggagga tttcagtatg ttcgataaag tgagaaaaac ggaagtgatc
     1921 agcaagttcg ggatcaatga aaaagacaca ggatctccgg aagttcaggt agctcttctc
     1981 acagaaagga tcaatatgct caccgatcat ttcaaggagc acaaaaaaga caaccactca
     2041 agactcggtc ttttgaaaat ggttggaaaa aggatgaagc tccttaagta cataaaagga
     2101 aaagattttg gtacatacaa cacacttatc aaggaacttg gtctccgtaa gtaaccttta
     2161 tattcaggtt ctttcaagaa aagtgtccat attttcaact gtcagatcaa cagaagtata
     2221 aaaaagagga aaaatgaaag agattaaaga aagcatcgtt tttaacggaa aagacctttc
     2281 catcgcaaca ggaagatatg caaaacaggc agacggagca atatggctca catacggtgg
     2341 aacggtactt cttgcaacgg ttgttgcatc aaaggaagaa gtttctgatg acattgattt
     2401 tttcccgtta acagttgatt atgttgagaa attttatgcc gctggtagat tcccgggcgg
     2461 ttttttgaaa agagaatccc agcagagcac tgcggagaaa cttacttcaa gacttattga
     2521 cagaccgctt agaccgatgt ttgccgactg gtttaagaac gaaacgcaga tacttgtaaa
     2581 ccttttaagt tatgattcag aatgtgatcc caaagttatt tcaatacttg cttcatctgc
     2641 ggcgcttatg gtgtcggata ttccttttaa cggacctgtt gccggatgcc ggattgttaa
     2701 aaagaacggt gaatttatca ttaatccttc tttaaacggt gagccggaaa aatatgaaat
     2761 agatatcact cttgccggaa accgcgaatc gatcatgatg gttgaaggag aagctcttga
     2821 agcatctgaa gatgatatga ccgctgcgat cgagatcggt cacaaggcaa ttcagccgct
     2881 tcttgacatt caggaaaaac ttgcaaaaaa gatctctcct gaaaaaagag ctgaaaaaga
     2941 gataaagatt gatgaaaaac tgaagaaatt tgttgaaaaa aatgccgctt cacttgtaaa
     3001 gaaagcactt tccacaacag taaaacttga aagatattcg cttgtgaaag aggcaaaaga
     3061 gaaggttgtt gaacttgtaa taaaagctct tgaagacggt gaaaagttca atgatgctga
     3121 taatcaggaa aaggttgtca agaggtcgaa gggctttttt gagaattatc tcaaggaatc
     3181 catgagagag atgatagtca agaaaaataa aaggatcgac ggcagagatc ttacttcgat
     3241 aagaccgatc accattgatc tcggagtgct tccgaaagtt cacggctcgg cagttttcac
     3301 aagaggcgaa actcaatctc ttggaactat aactctcggt atcggacatg atgagcagag
     3361 agttgataca gttactgagg aagacagcaa gaacttcatg cttcattata atttccccgg
     3421 tttttctgtg ggtgaagtag cacggcttaa atctccggga agaagagagc tcggtcacgg
     3481 caatcttgct gaaagagcac tttctccgat aattccttca aaagatgagt tcccttacac
     3541 gatcagagtc gtttctgaga tacttgagtc aaacggttcg tcttcacagg cgactgtatg
     3601 ttcaggttct cttgcgctga tggatgccgg aatacctttt aagaaacatg ttgcaggcat
     3661 tgcaatggga cttataaaag agaaaaacga ttttttcatt ctttccgata tattaggcga
     3721 cgaagatcat cttggcgaca tggatttcaa ggttgcaggg acaaaagacg gcattacagc
     3781 gatccagatg gatattaaga tagaaggtct tcccaaagat gtgatggaaa gggcaatgat
     3841 ccaggcaaaa gtcggcagaa tgcacataat cggtgaaatg gacaaggcga ttactggtcc
     3901 aagatcagaa cttaatccca acgctccgaa acttctcaag atgaagattg aagttgagaa
     3961 aataaaagac cttatcggaa caggcggaaa aaatatcaga tctattgttg aacagactgg
     4021 agccgatatt gaagtcgaac aggatggaac agtgaaaatc gctgcaaaag accagcagat
     4081 acttgacctt acaatagcac ttgtaaaatc tttcacagac agtgtcgaac ttggaaagat
     4141 ctatgaaggt gaagtgacaa ggatagaaga atacggcgct tttgttgaaa taatgaaagg
     4201 aacgactggt cttctccatg tcagcaagat agctgatgaa agggttgaaa atgttactga
     4261 ttatgtaaaa ctcggtgaca aagttaaagt tatcgtcaca gaaattgaat tcggcggaaa
     4321 gttcaagctc agcatgaaac cgactgattt cgaaaaagat tggaaaaagg aaaaagagaa
     4381 tagaccgccg aaaagagatg accgtaaagg cggacacgac agaaacgacc gcgggagaga
     4441 ccgtgacaac cgcagacgct gatattttaa tcaaattcaa actggagaac ggagccgttc
     4501 ttcctaaata cgaaacagaa cattcatccg gaatggatgt caggtcagtt gaaaattgtt
     4561 taatagatcc aaacagttgg aaacttattt cgacaggact tttccctgaa atcccagttg
     4621 gttatgaaat acaggtaaga tcaagaagcg ggtt
//
LOCUS       DAOOYB010000231         4623 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_21760_length_4623_cov_10.277364, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000231 DAOOYB010000000
VERSION     DAOOYB010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4623
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21760_length_4623_cov_10.277364"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2016
                     /locus_tag="PL056_11225"
     CDS             <1..2016
                     /locus_tag="PL056_11225"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HPM47630.1"
                     /translation="CNNIFALIKNVVYLFIYKDGAITKREKTPELLQKLFKFIPDSSK
                     ETDRIFRLAAAAFIILTFISVPLAVLSSGSSSDFEEALSHYFYYLSTFSFVFFTAAVV
                     IYYTFSKKARTVLCATALFLLLYGLVNTFVFAGNYGDMSHFVFNEELEIEMLEILLNI
                     FSGVIIAGAVFLLLKKWIRFLRPALSVIIMSLILFSINESFAYKDKRTNILIAQIDLK
                     DNHYAFSKTGKNVVVIMLDRFIGGYMPQILELMPNLNSDLDGFVYYRNSLSQASYTIG
                     GVPPILGGWDYNVHNANILRTEVPLLKKLDESSRILPYNFDKNGYSVKIYRTDCINWF
                     DKSTNWVDENNKEYIGNTKFIEPDYPKYREMWLQKNEKESGNDDTLRKKLLTFGLFRV
                     SPLFVREFIYDNGEWHIESDVENEENYEKPDEKEFVIANQKSKYRRDTTLKYYAALDF
                     LPEMSRVLEEEKGQFIFLSNNLTHEPHSINSDFEFEITGKVAYPRSVFNKFRRSLTAV
                     RHLYTDAAALRLVNKWIQWMKDNGVYDNTRIIIVSDHGRDVYNPFFKQQKIPNTRKKA
                     HPSYYNNLFLVKDFNAKGELVINTEDFMTSADVPALAVKDIIPDATNPYTGKKIEMPG
                     NKFPFYIYDVQWRVEKQDKFKYRYHEKYMIEKFEDIDSPSKWRRIYE"
     gene            2009..3292
                     /locus_tag="PL056_11230"
     CDS             2009..3292
                     /locus_tag="PL056_11230"
                     /inference="COORDINATES: protein motif:HMM:NF016357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HPM47631.1"
                     /translation="MNKINVLTYLLFMVILPLPLFAYLDPGSGSMLFSAVIGIVATLF
                     FVIKGVFFKIIDLPAYFSGVSRGKRAKHSIVFYCEGPQYWNIFLPVIKELSSRNVKCT
                     YLSSKENDPGLSCGFPNVETKFIGEGNKAFFYLNTLSADVCIMTTPGLDVLQIKRSKG
                     VKKYIHIPHSTGGGCSGYATYGTDYYDAVLTGGDSDIEFIRELEQVRGLPAKELVAIG
                     CTYMDVLREKASKITTPASDRITILLSPTWGLHGLLRKYGDKVLTYLTNLNKYNIIVR
                     PHPQGWISDKDLINELKNKFPDGEHLVWDSAPDGTESMKTADIMISDFSGIIFDFILL
                     FEKPVLAFKGNYDKRGHDSMDIKEDPWVLQAVERTGISIEEKDVEDLESRINEKLEDK
                     AEFVNHLEFIHNSMDKFPGESGARGADEITRIAGS"
     gene            3307..4596
                     /locus_tag="PL056_11235"
     CDS             3307..4596
                     /locus_tag="PL056_11235"
                     /inference="COORDINATES: protein motif:HMM:NF014186.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HPM47632.1"
                     /translation="MEHLFTIFIRPLTVLMEFILENIVKWSGSYFISLILLSFFVTIV
                     AMPFSVVAERLQEHERAVQKKMQPKIKEFRAVFKGSVLNRYITTLYRQNRYHPVLAVR
                     SSLGFLIQIPFFFAAYVFISDYQQLNGISTLIFENLGEPDGLIQVAGFNINIMPFVMT
                     AANLGYAFVYGKNLSLKEKINSYVISALFLALLYNSSSALLIYWTSNNIFGIVKSIMF
                     KYTGQKKIPLLTSGKVSPDYLYGFFISLFSFAHLTFIAVPFLLLASGSGSDIEGTFSQ
                     IFSFQLMLFAVSVTILSTLFYYFSGPVKKIFAVMMTVISLYAFANVFLFPGDYGDITN
                     FVFEDGIQISFADTLPNLAALVLISVATMILIYLKKNRSLNMILSFLFLSLFIFSIHE
                     GMIFYEKSGSEDLSPTSELEKKFVFSTNRNQNRSILT"
BASE COUNT         1423 a          919 c          889 g         1392 t
ORIGIN      
        1 tgcaacaaca tttttgctct tatcaagaat gttgtttatc ttttcatata taaggacggt
       61 gctataacaa aaagagaaaa aacaccggag ttattgcaga aactgttcaa atttattccc
      121 gattcctcga aagagaccga caggatcttc agacttgcgg cagcagcttt tataatacta
      181 actttcatat cggttcctct tgcagtcctt tcttcggggt cgtcttctga ttttgaagaa
      241 gctttgtccc actattttta ctacctttcg actttttcat ttgttttttt cacagctgcc
      301 gttgtgatct attatacttt cagcaaaaaa gcacggactg ttctttgtgc gacagcactt
      361 ttccttcttt tatacggact tgtaaacact tttgtgttcg caggcaacta cggtgacatg
      421 agtcattttg tatttaacga agagcttgaa atagagatgc ttgaaatcct tttaaacatc
      481 ttttccggag tgataattgc cggtgcggtt tttcttcttc ttaaaaaatg gatccggttt
      541 ttaagaccgg cactttcagt cataataatg tcgctcatcc tcttttcaat aaacgaatca
      601 ttcgcataca aggataaaag aacaaatatt ttaatcgctc agatcgatct taaagacaat
      661 cattacgctt tttcaaaaac agggaaaaat gttgttgtta tcatgcttga caggttcatc
      721 ggcgggtata tgcctcagat ccttgagctt atgccgaacc tcaattcaga tctcgacgga
      781 tttgtctatt acagaaattc tctttcccag gcaagctaca ctatcggcgg agtccccccg
      841 atcctcggcg gctgggacta taatgttcac aatgcgaata ttttgagaac agaagttcct
      901 ctccttaaaa aactggatga gagctccaga atacttccgt ataacttcga caagaacgga
      961 tacagcgtga aaatttaccg gacagactgc ataaactggt ttgacaaaag caccaactgg
     1021 gttgacgaga acaacaagga atatataggc aacaccaagt tcattgagcc cgactatcct
     1081 aaatacagag agatgtggct tcagaaaaat gaaaaggaaa gcggtaatga tgatacgctc
     1141 agaaaaaaac tgctcacatt cggactgttt cgggtttcac ctctttttgt aagggaattt
     1201 atctacgaca atggcgaatg gcatatcgaa tcagatgtgg aaaatgagga aaattatgaa
     1261 aaaccggatg agaaggagtt tgtgatcgca aaccagaaaa gcaaatatag aagagacaca
     1321 actctgaaat attatgccgc acttgatttt cttccggaaa tgtcgagagt tcttgaagag
     1381 gaaaaaggac aattcatttt tctttcaaat aatctgaccc atgaacccca ttccataaac
     1441 agcgattttg aatttgagat aactggaaaa gttgcatatc ccagatcagt atttaataaa
     1501 tttcgcagaa gcttgacagc agtcagacac ctttatacag atgctgcggc acttcggctg
     1561 gtcaacaaat ggatccaatg gatgaaagac aacggggttt atgacaatac aaggataatc
     1621 attgtttcag accacggaag agatgtgtat aatccttttt tcaagcagca gaaaatacct
     1681 aataccagaa aaaaggccca tccttcatat tacaacaacc tttttcttgt aaaagacttt
     1741 aatgcaaaag gtgaacttgt tataaacact gaagatttta tgacatcggc ggatgttccg
     1801 gcactagcgg tcaaagatat aatccctgat gcgacaaacc cttacaccgg aaaaaagata
     1861 gaaatgccgg gaaataaatt tcctttctat atttatgatg ttcagtggcg tgttgaaaag
     1921 caggacaaat ttaaatacag atatcatgaa aaatatatga ttgaaaaatt tgaagatatt
     1981 gattcacctt caaaatggag aaggatctat gaataaaata aatgtcctta catacctgct
     2041 ttttatggtc atactgcctt tgcctctctt tgcatatctt gatcccggtt caggaagtat
     2101 gctgttttcg gctgtcatag gtattgtcgc gacacttttc tttgtcataa aaggtgtatt
     2161 ctttaaaata attgacctcc cggcttattt ctcaggcgtc agcagaggaa aaagggcaaa
     2221 acattcaata gtattttact gcgaaggtcc tcaatactgg aatatttttc ttcctgttat
     2281 caaagaatta agctcaagaa atgttaaatg cacatacctt tcaagcaaag agaacgatcc
     2341 gggtttaagc tgcggatttc ccaatgtgga aaccaaattc atcggtgaag ggaataaagc
     2401 attcttttat cttaacacac tttcagccga tgtatgcata atgacaactc ccgggcttga
     2461 tgttcttcag ataaaaagaa gcaagggtgt aaaaaaatac atacacattc ctcactcaac
     2521 aggtggcggg tgcagcggtt acgcgactta cggaacagac tattatgacg ctgttctcac
     2581 aggaggtgac tcggacattg aatttattcg cgaacttgaa caagtgcgcg gactgccggc
     2641 aaaggagctt gtcgccatag ggtgcaccta tatggatgtt ctccgtgaaa aagcttctaa
     2701 gatcacgacc cctgcttcag acaggataac aatacttctt tcccccacat ggggacttca
     2761 cggactgctt agaaaatacg gtgataaagt tctcacatac ctgacaaatc tcaataaata
     2821 caatatcata gtcaggcctc atccgcaggg ctggatatca gataaagatc tcatcaatga
     2881 actgaaaaat aaatttcctg acggggaaca tcttgtctgg gatagcgctc ctgacggcac
     2941 agaatcaatg aaaacagctg acataatgat aagtgacttt tcaggaataa tatttgattt
     3001 catcctgctt tttgaaaaac cggttcttgc cttcaaagga aattatgaca aacggggaca
     3061 cgattcaatg gatattaaag aagatccatg ggtgctgcag gcagttgaaa gaacagggat
     3121 atcgatcgaa gaaaaggatg ttgaagatct tgaaagccgt atcaatgaaa aacttgaaga
     3181 taaagctgaa tttgtgaatc atcttgaatt tattcataac agcatggata aattccccgg
     3241 agagtcgggg gcaaggggcg cagatgaaat aacaaggatc gcaggatctt agataaggac
     3301 agctttatgg aacacctttt caccatattt attcgacccc ttacagttct tatggaattc
     3361 atccttgaga atattgttaa atggagcggt tcttatttta tatctttaat attactgagt
     3421 tttttcgtga ctattgtggc aatgcctttt tcagttgtcg ccgaaagact tcaggaacat
     3481 gagagggctg ttcagaaaaa aatgcagccg aaaattaaag agttcagagc cgtttttaaa
     3541 ggttcggttc tcaacagata cataactacg ctttacaggc agaaccgtta tcatcctgta
     3601 ctcgcagtga gatcatcact ggggtttctg atccagatac cgttcttctt tgccgcatat
     3661 gttttcatct cggattatca gcagttaaac ggcatttcaa cactaatatt tgaaaatctc
     3721 ggagaaccgg acggactcat tcaggtcgca ggatttaata taaatataat gccttttgta
     3781 atgactgccg caaacctggg atatgctttt gtttacggaa agaatctctc tcttaaggaa
     3841 aagataaaca gttatgtgat ctcggcacta ttccttgcac ttctttacaa ttcttcatcc
     3901 gcacttctga tatactggac ctctaacaac attttcggaa tagtgaaaag cataatgttc
     3961 aaatatacag gacaaaagaa aatacctctg ctcacttccg gaaaagtttc accggattat
     4021 ctctacggtt ttttcatttc gctgttctcg tttgcccatt taactttcat agctgtccct
     4081 tttcttcttc ttgcatcggg ctccggctct gatatcgaag gaactttttc gcagatattt
     4141 tctttccagt taatgctgtt tgctgtttct gtaacgatct tgtccactct tttttattat
     4201 ttttcaggac cagtgaaaaa gatctttgcc gtcatgatga cagtcatctc tctttatgca
     4261 ttcgcaaatg ttttcctttt tccgggagat tacggtgaca taacaaattt tgtatttgag
     4321 gatgggattc agatctcttt cgcagacaca ctgcccaatc ttgctgcact tgttcttatt
     4381 tcagttgcga caatgatttt aatatatctg aaaaaaaacc ggtcactcaa catgatcctc
     4441 tcttttctgt tcttatctct tttcattttt tcaattcatg aagggatgat cttttatgaa
     4501 aaatcaggat cggaagatct ttccccgaca tcagagcttg agaaaaaatt cgttttttca
     4561 actaacagaa accaaaatcg ttcgattctg acttaaactc ccgaaaaatg gtctgagctc
     4621 agc
//
LOCUS       DAOOYB010000232         4598 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_21929_length_4598_cov_8.720009, whole genome shotgun sequence.
ACCESSION   DAOOYB010000232 DAOOYB010000000
VERSION     DAOOYB010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4598
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21929_length_4598_cov_8.720009"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(224..4447)
                     /locus_tag="PL056_11240"
     CDS             complement(224..4447)
                     /locus_tag="PL056_11240"
                     /inference="COORDINATES: protein motif:HMM:NF014466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HPM47633.1"
                     /translation="MKINFFVRFELFLIFFMVVPFNLFSTDTPEEREISEDSEIPQDY
                     VFEFVREDDGSVYTAPENEGNGSGDNKDHETQGIAAIGEDYWILAKNKYIDTFVFNKY
                     NYFSDEEDKVDNFNPESKAGDIDFSDGFLYVPDDKLRIYEWFENHQSSKYQQVAALDW
                     DYDLPYATVFPYRKIVFTDFDSGNAVFEGVEVKENDEEWEKGSCSLKIKFKDKEGNIH
                     NPCSAGDSYCTQGADFSPNGRFIFYVRDFSVATYVEAFLEALNGGGVFNPNDVNVDPT
                     GIYVYYIPDEDFIKLKNNICPENIKAVNVGFENILFNPIASIIDVFGDIKVIRDKELE
                     GIEISSLGKYDIHQLMLNNTSGSNESHTIYHWMSGDYDEDNIKDINDNCPFVKNYNQK
                     DWNKDGIGDACQDSDEDGIMDEDDNCLETYNSDQNNWNGGVGDACQDSSGDGWMDDMS
                     ACPNLDMRKYFPDMVYVLNNQAVDITTKSCRRSTDLMCVNIRAVSDYCDMDGDGRWDL
                     EPEDEYEIDEYAYSASGLWSKLGENVNIENCKGISCFGTQEIGHKLSVTQRASYFCGQ
                     NDTKSKLSTYFCYCGKNDDEHLQCKDNGNCGKDHANPNKVWDEKVILSPNGQSTWQPV
                     FSEFQGINTNSEGCWKSGGKFDKIKKLSKIKEWDYKTDKYLKKLLTGDDFLVHKDGES
                     VRQNGNYTMLKLAQGVVTGSSSSYVTTTASGGHNLNKNYFSNYNQTHVLGNKAELILA
                     KQNSEYVDIIIEEVPYLRIRVNPELGGYGKWWYDMMRDYIGIKPWDDPRWEQGFEDRV
                     KHSIYESISRPSEFSKVGFDGNSINLESVSFKDDYNQIFSFGGRDAAYFMKDGVFSIA
                     FTDVNGIFNDVSPVAYGIEMRSAAALTSGTEIFMVAGFKERTARTSSEHADINSAEQM
                     PERSASFAKISFSDNGAYMTTLSPLPWEPVHISLFEVEGRIHSIMMDGNGTGEILVYY
                     SENEYWEKLSDFNFGKVFSLNNTFVKGNELYFTAPGPNDQNTLYSWSSEFGINEVASL
                     GISYDAFIKPLNSGEKIILADLKDISGNIGAWELSEGEFTPVSIQIEKPEYETAFCIS
                     EINGSIFPGATNYKGQCISVYDYNYQTVSYLDYKKTVAGHKNNLYIGGLTGVRRVEIN
                     EDGSLSNSDMLYTGETNNLAVYGNTMYGANYGEIDIYAIAENGSISRVKGISSSSCGN
                     VRVSGDMLFTAENKKVRVFDLTDPQNPTLVKTITTAGKVIDLEVIGERLYIYEETTSW
                     LTTKGFTGIYDISNLNSPVKTKYFEKRCTDAEMQRSGETIYLGCKNGQYRIEETGLKG
                     ITGEKNFVREGYVHNKVLYQVYSGALHKSKVSGIQAECGNGMIEPGEICDSDTIQCTE
                     LDSSYIGGTASCNSACDGYNESVCETDGW"
BASE COUNT         1272 a         1059 c          712 g         1555 t
ORIGIN      
        1 cctgcgaatc atggtcgttt ttcggatcat ttgagttaca tgaagtgcat gaccaaaatg
       61 acagcaaaag gaatataatt aatatgttac gcataaaaaa ccctccaaat aactcgctcc
      121 ttccatctct ttcaacccat tccctaaatc cctttccctt tcaaaaagga aagggactta
      181 aaactcccct tctttccgta gagagaaggg gtcaggggat gagttaccat ccgtccgttt
      241 cacaaacact ttcattgtat ccgtcacatg ccgaattgca agatgctgtg cctccgatgt
      301 aacttgaatc aagttccgtg cactggatcg tgtcgctgtc acatatttca cccggttcga
      361 tcattccatt tccgcattcc gcctgaattc ctgatacttt tgatttatga agtgctcctg
      421 aatatacctg ataaagaact ttgttgtgta catatccttc tctgacgaag ttcttttctc
      481 cggttatccc tttaagtcct gtttcttcaa ttctgtactg accgttcttg caaccaagat
      541 aaatagtttc acctgatctc tgcatttccg catctgtgca tctcttttca aagtatttcg
      601 ttttcacagg tgaattcagg ttgctgatgt cataaatgcc tgtaaatcct ttcgttgtca
      661 accagcttgt cgtttcttca taaatataga gccgttctcc gattacttca agatcaatga
      721 ctttgcccgc cgttgttatc gttttaacaa gtgtcggatt ctgcggatct gtcaggtcaa
      781 acactctaac ctttttattt tccgctgtga aaagcatatc accggacact ctcacattgc
      841 cgcatgatga tgaactgatc cctttcactc ttgaaattga accgttttca gcgattgcgt
      901 aaatatcaat ttctccgtaa ttggctccgt acattgtgtt cccatacact gcaagattgt
      961 tcgtctcgcc tgtgtagagc atatcgctgt ttgaaagcga tccgtcttca tttatctcaa
     1021 ctcttcttac tccggtgagt ccgccgatat acagattgtt tttatgccct gcaacagttt
     1081 tcttataatc aagatatgaa accgtctgat aattgtaatc ataaactgaa atgcactgtc
     1141 ctttgtaatt tgtggcaccg gggaatattg aaccattgat ttcactgatg cagaatgccg
     1201 tttcatattc cggtttttcg atctgtatgg aaacaggtgt gaattctcct tctgaaagtt
     1261 cccatgctcc tatatttcct gaaatgtcct tgagatctgc aaggattatc ttttctcctg
     1321 aattgagcgg cttgatgaat gcgtcgtagc tgattccaag cgatgcaact tcatttattc
     1381 caaactcact gctccatgaa taaagcgtat tctgatcatt gggtccggga gctgtaaaat
     1441 aaagctcgtt cccttttacg aatgtattat tgagcgagaa cactttcccg aaattgaaat
     1501 cactgagctt ttcccagtat tcattttcac tgtagtacac gagtatttca ccggttccgt
     1561 ttccatccat cattattgaa tgaatccttc cttcaacttc aaatagagaa atatgaaccg
     1621 gttcccatgg aagcggtgaa agtgttgtca tatatgcacc gttatctgaa aatgatattt
     1681 tagcaaaact tgcagatctt tcaggcatct gctcagcact gttaatatca gcatgctcac
     1741 tggaagtccg tgcggttctt tccttaaatc ctgccaccat gaatatttcc gttccggaag
     1801 taagtgccgc tgccgatctc atttcaattc cataggcaac aggactgaca tcattgaata
     1861 ttccgttgac atcggtaaat gcaatgctaa atacaccgtc tttcatgaaa tatgcggcat
     1921 cccttccgcc gaaactgaat atctgattgt aatcatcttt gaacgatact gattcaagat
     1981 ttattgaatt gccgtcaaat cccactttgg aaaattccga cggtcttgat atggattcgt
     2041 aaatgctatg cttaactcta tcttcaaatc cctgttccca tcttggatca tcccagggtt
     2101 tgattccgat ataatccctc atcatgtcat accaccactt gccgtatccg cctagttcgg
     2161 gattaactct gattcttaaa taaggaactt cttcaattat tatgtcaaca tattctgaat
     2221 tctgttttgc gagaatcagt tcggctttat tacctaaaac atgtgtctga ttatagttgc
     2281 tgaaatagtt tttgttcaaa ttatgcccgc cggaagctgt tgttgtaaca tagctggaac
     2341 tactcccagt gaccactccc tgtgcaagtt tcagcatcgt ataattaccg ttttgccgga
     2401 ccgattctcc gtctttatgt accaaaaaat catcaccagt cagaagtttt ttaagatact
     2461 tatcagtttt gtaatcccat tctttaattt tagagagctt ttttatttta tcaaatttac
     2521 cgccactttt ccaacatcct tcactgtttg tgtttattcc ctgaaattca ctgaatacag
     2581 gctgccaggt tgattgaccg tttggtgaaa gaatgacttt ttcatcccac actttattcg
     2641 gatttgcatg gtctttgccg caatttccgt tgtctttgca ttgcaggtgt tcatcatcat
     2701 tctttccgca atagcagaaa tatgtagaaa gttttgattt tgtatcgttc tgaccgcaga
     2761 aataacttgc acgctgtgtt acagaaagct tgtgaccgat ctcctgtgtc ccgaaacatg
     2821 aaataccttt acagttttca atgttcacat tctcacccag cttgctccat aatccgctcg
     2881 ctgaatatgc atattcgtca atctcatact catcctccgg ctcaagatcc caccgaccgt
     2941 caccgtccat gtcgcaataa tctgatacag ctcttatatt aacgcacatc agatcagttg
     3001 atcttctgca ggattttgtc gtaatatcaa ctgcttgatt gtttaaaaca tataccatat
     3061 ccggaaaata ttttctcatg tctaaattag gacacgctga catatcatcc atccatccgt
     3121 caccggaaga atcctgacat gcatcgccaa ctcccccgtt ccagttattc tgatcggaat
     3181 tgtaagtttc taaacaattg tcatcttcat ccataattcc gtcttcatca gaatcctgac
     3241 acgcatcgcc aatgccgtct ttgttccaat ctttctgatt gtaattctta acaaacgggc
     3301 agttgtcatt aatatctttt atattatcct catcataatc tcctgacatc cagtgatata
     3361 tagtatgaga ttcattcgat ccacttgtgt tattcagcat aagttggtga atatcatatt
     3421 tcccgagaga cgaaatctca attccctcaa gttccttatc ccttataact ttaatatcac
     3481 caaatacatc aattatagaa gctattggat taaataaaat attttcaaac ccaacattaa
     3541 cagcttttat attttccgga caaatattgt ttttaagttt tataaaatct tcatcaggaa
     3601 tgtaataaac ataaattcct gtagggtcaa cattaacatc attggggtta aagacccctc
     3661 ccccattaag cgcttcaaga aacgcttcga cataagttgc aacagaaaaa tctctcacat
     3721 aaaatataaa ccttccatta ggagaaaaat ctgcgccttg agtacaataa ctatctcctg
     3781 cactgcatgg attatgtata tttccttctt tatctttaaa ttttattttt aaactgcatg
     3841 atcctttttc ccattcttca tcattttcct taacctcaac accctcgaaa acagcatttc
     3901 ccgaatcaaa atctgtaaac actatttttc tataagggaa tactgttgca taaggcaaat
     3961 cataatccca atccaaagct gctacttgct gatattttga tgattgatga ttttcaaacc
     4021 actcataaat tctcaactta tcgtcaggta catataaaaa tccatcagaa aaatcgatat
     4081 cgccagcttt tgattctgga ttgaagttat ccaccttatc ttcttcgtca gaaaaataat
     4141 tgtatttgtt aaacacaaaa gtatcaatat acttgttttt tgctaaaatc cagtaatctt
     4201 caccgatagc agcaattcct tgtgtctcat gatctttgtt gtctccactt ccattccctt
     4261 cgttttcagg tgccgtataa acactgccat catcttctcg cacaaattca aaaacataat
     4321 cctgcgggat ttcactgtct tcggaaatct ctctttcctc aggagtatcc gtagaaaata
     4381 aattaaatgg aacaaccata aaaaaaatta agaacaattc aaatctgaca aaaaaattaa
     4441 ttttcattac cccttctcct ccatcatgaa aaataataaa aatatcccgt gaaaaactct
     4501 tgaaatactt gaacgtatct gaaaaaacta aaggattgtc ttgtcttgtc tcttgtctct
     4561 tgtctcttgt ctcttgtctc ttgtctcttg tctcttgt
//
LOCUS       DAOOYB010000233         4583 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_22023_length_4583_cov_7.364399, whole genome shotgun sequence.
ACCESSION   DAOOYB010000233 DAOOYB010000000
VERSION     DAOOYB010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4583
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22023_length_4583_cov_7.364399"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="PL056_11245"
     CDS             <1..582
                     /locus_tag="PL056_11245"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPM47634.1"
                     /translation="YREFIFNGEKHVSLLSFEEARDRVIMMDSISKRFSSCGARIGAF
                     ISYNCDIMKAALAYGQARLCPPTIDQLMAVKSVDLDENYYSQVVGEYKKRRDVVLKAL
                     EGMEGVEYRVPNGAFYIVIKLPVENAEDFVIWLLTDFHVDNETVMLAPAEGFYSTPGL
                     GRNEARIAFVLNEQKTERAMMILKKGLEKYKSL"
     gene            687..3833
                     /locus_tag="PL056_11250"
     CDS             687..3833
                     /locus_tag="PL056_11250"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47635.1"
                     /translation="MKNIRFFVFMLCILSVSALSATIEGTMIGGEPYERWEDGGQDFF
                     VMFNSNIDNKLVLFEEDPENPQGDTCVDESTFTLDDFHVPKDSIVEKAYLVWMGAVDP
                     SKLDQPTDNSVKLKFIQTAETSPVIYEESITAGTEGKLLSEGDEDSEKTFEFESLYFQ
                     DTVENGCTETAGGSIVRDQYLGYFTYRVDITSFFDKISEMNNEAGKLEEGQYFGTYVF
                     SDLECTEHDYYRCKTTMVSAWAIFIVYRSQNIRPKKLYLYNGLAFVQGDKSLASVSGF
                     ELPKNPMVRLTSMIAEGDPSLVEASLPPEGIFLQGEGATSRFRLFNECNPLMGTYVEV
                     FNSKSSVVNWNPDAEEKIQCISGPDDEWANYGIDVDTFLLDSETNINLQEHLKKGNES
                     MEITLSVNQDAIFTNFMVLSVDNKGANFDIPPEAADTTKSKLNFPLDREKHFCACPST
                     DEGKIEDYYCETVNSRRDFYYFIKVQNWGDEDAEDVVVTDELDKNLEYIPGTTSMATV
                     YDAEKDTYTDWTVIPDKSDGGFPLSGSGFKVSSKMRNCDQSKWTCQDTVLIRYKVKPK
                     SGIAKNYTFQNMALIKDKKSDEAYKTNLSYPLKLKPTTCEIDTTCPSPTEEMCGGVPE
                     DECDCGEKADGATCSDGYACDGCYCVDDPAKTCFNSTAEFDLGKNSPLSDGSKIIIPK
                     DNEGQKLVVGQFTLQVTNCEDRFFNFDAATIHYETMGDTNFSFKNLELIYDFDGNGVY
                     DEAVDTILATGEVFTNYVKFFIDPGHKKYLGKNIHYFLVRTDVNYNAEEIVQGTIFNF
                     LIESENSIEISDQGTTMVSQNDVEFATFMLEPTGDFFIVTIGPKDPPVPAFSEINKNI
                     PVLQLKTKAAMRANTIEKFTIKIPSAGGYVKFGEENGISEISLWVDTNKDGTGDLKVA
                     EQTKFETVSTTVMFSDFIQPISYLEGEEKYLVVNVKFNMVKADDPMSGRIEIPNSGIT
                     VVDDEASVVELPIRSKIFRYECKEGDVGCEVPPIPKPDGCSCATVGVDSSDSNALLVA
                     ALAVLAALSALLLRKRIA"
     gene            3981..4196
                     /locus_tag="PL056_11255"
     CDS             3981..4196
                     /locus_tag="PL056_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47636.1"
                     /translation="MKNITIGLDARTFFLIKQANPKNLSKFIRDAIAEKAQAELNRQI
                     DDYCANLKSSMEDFDGFNSESEENWPN"
     gene            4156..4518
                     /locus_tag="PL056_11260"
     CDS             4156..4518
                     /locus_tag="PL056_11260"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HPM47637.1"
                     /translation="MDSTANQKKTGQIKRGAIVIVQFNPSVGSETKNIHFAVVVSIDQ
                     LNSRSPLISVVPMTTKVDRFFPAFEVMINETGKPAKADCAQVRTIAKERVVEIKGTVS
                     EETLIEIGNKIKMALGLD"
BASE COUNT         1425 a          854 c         1033 g         1271 t
ORIGIN      
        1 tacagggaat tcattttcaa cggtgagaaa catgtcagct tgctcagctt tgaagaggca
       61 cgggacagag taataatgat ggacagcatc tcaaaaagat tctcttcatg cggagcgcgg
      121 ataggagctt tcatcagcta taactgtgat ataatgaagg cggcgctggc atatggacag
      181 gcaagattgt gtcctccgac gatagatcag ctcatggctg tcaaatcggt cgatcttgat
      241 gaaaattatt acagtcaggt tgtcggagaa tataagaaaa gaagggatgt cgttctgaag
      301 gcgcttgaag gaatggaagg agttgaatac agagttccga acggtgcttt ttatatcgtt
      361 ataaaacttc ctgttgaaaa tgctgaagat ttcgtgatct ggcttcttac cgattttcat
      421 gtggataacg aaacagtgat gctcgctccg gcggagggct tttattcaac acccggactt
      481 ggcaggaatg aagcaagaat agcgtttgtt ctgaatgaac agaaaacgga acgggctatg
      541 atgatactta aaaaaggtct tgagaaatac aaatctcttt aacataacct tgctttttaa
      601 tacaatctaa attagaatct ctacaatgtg ttttttgtgt tgggatcaat atgcgaaata
      661 tagttttaag tttcacagga gggattatga aaaacattag attttttgtg tttatgcttt
      721 gtattctttc ggtttcggct ttatccgcca ctatcgaagg gacaatgatc ggtggagaac
      781 cgtacgaaag atgggaagac ggcggacagg acttttttgt gatgttcaat tcaaacattg
      841 acaacaaact ggttcttttt gaagaagatc ctgaaaaccc gcagggagat acatgtgttg
      901 atgaaagcac tttcacgctt gatgatttcc atgtgcccaa ggacagcatt gttgaaaaag
      961 catatcttgt atggatgggc gctgttgatc cttccaaact tgatcagccc accgataaca
     1021 gtgtgaaact taaattcatc cagactgctg aaacttcgcc tgtaatttat gaagagtcaa
     1081 taaccgcagg aactgaggga aaacttctta gtgaaggtga tgaagattct gaaaaaactt
     1141 ttgagttcga atctctctat tttcaggaca ctgttgagaa cggttgtact gaaacagccg
     1201 gtggctcaat tgtcagagat cagtatcttg gatattttac ataccgtgtt gatatcactt
     1261 cattttttga caagatatct gaaatgaaca acgaagcggg gaaacttgaa gaaggacagt
     1321 atttcggaac ttatgttttc agcgatcttg aatgcaccga gcatgattac taccgctgta
     1381 agacaacgat ggtcagtgca tgggcgattt tcattgttta ccgttcacag aacatcaggc
     1441 cgaaaaaact ttatctttac aacggacttg cctttgttca gggtgacaaa tcacttgcat
     1501 cggtaagtgg atttgaactg ccgaaaaatc cgatggtgcg gcttacatca atgatagcgg
     1561 aaggtgaccc gagtcttgtt gaggcatcat tgccacctga aggtattttc cttcagggtg
     1621 aaggtgccac aagcagattc cgtcttttca atgagtgcaa tccgctcatg gggacttatg
     1681 tagaggtttt taattcaaaa tcttcagttg taaactggaa tcccgatgca gaagaaaaaa
     1741 tacagtgtat aagcggtccg gacgatgagt gggctaacta cggtattgat gtcgacacct
     1801 tccttcttga cagcgaaacg aacattaatc ttcaggaaca cttgaagaaa gggaacgaat
     1861 caatggagat aactctttct gtcaaccagg atgcgatatt caccaacttc atggtgcttt
     1921 ctgttgataa caaaggtgca aatttcgata ttccgcctga agcggctgac acaacaaaat
     1981 caaagcttaa tttcccgctt gacagggaaa aacatttctg tgcctgccct tccacagatg
     2041 aaggcaagat cgaggattac tactgcgaga ccgttaacag cagaagagat ttctattact
     2101 tcatcaaggt tcagaactgg ggagatgaag atgctgaaga tgttgttgta acagatgaac
     2161 ttgacaaaaa tcttgaatat attcccggaa ctacatcaat ggctacagtt tatgatgcag
     2221 agaaggatac atatacagac tggacagtca ttccggataa atcggatgga ggatttcctc
     2281 tttccggctc aggttttaag gtcagcagca agatgagaaa ctgtgatcag agcaagtgga
     2341 catgtcagga cactgttctg ataagataca aagtgaaacc gaaatccggt attgcaaaga
     2401 actatacatt ccagaacatg gctcttatca aggacaagaa aagtgatgaa gcatacaaaa
     2461 ctaatttgag ctatcctctt aaattgaaac ccacaacatg cgaaattgac acaacatgtc
     2521 cttctcccac tgaagaaatg tgcggtggag tgccagagga tgagtgtgat tgcggtgaaa
     2581 aagcagacgg agcaacctgt tctgacggtt acgcatgtga cggatgttac tgtgttgatg
     2641 accctgcaaa gacctgcttt aattcgactg ctgagtttga tcttggcaaa aactctcctc
     2701 tttccgacgg gtcgaagata ataattccga aggataatga aggacagaaa cttgtcgtcg
     2761 gtcagttcac tctccaggta acaaactgtg aggatcgttt cttcaatttt gatgccgcga
     2821 cgatacatta tgaaacaatg ggcgacacca atttcagttt caaaaatctt gagctcattt
     2881 acgattttga cggaaacggt gtatatgatg aagcagttga cacaatactt gccacaggcg
     2941 aagtttttac aaattatgtt aagttcttca ttgatccggg tcacaagaaa tatcttggaa
     3001 agaatataca ctatttcctt gtcaggactg atgtaaatta caacgctgaa gaaatagttc
     3061 agggaacgat cttcaatttt ctcattgaat ctgaaaactc aattgaaata agtgatcagg
     3121 gaaccacgat ggtctcccag aacgatgtag aatttgcaac cttcatgctt gagccgacag
     3181 gagatttctt cattgtaacg atcggaccga aagatcctcc tgttcctgca ttttctgaaa
     3241 taaacaaaaa tattccggta cttcagctta agacaaaagc ggctatgaga gccaacacca
     3301 tagaaaaatt cacaataaag atcccgagcg cgggaggata tgtgaaattc ggagaagaga
     3361 acggaatttc tgagatttcg ctctgggttg acactaacaa ggatggtacg ggtgatctca
     3421 aagttgctga gcagacaaaa tttgaaacag tttcaacaac agtgatgttc agtgatttca
     3481 ttcagccgat ctcctatctt gaaggagaag aaaaatatct tgtggtaaat gtgaagttca
     3541 acatggtgaa agctgatgat ccgatgtcag gaagaataga aataccgaac agcggtataa
     3601 cggtcgttga tgatgaggca agtgttgtcg aactcccgat cagatcaaag atattcagat
     3661 atgaatgcaa ggaaggcgat gtcggctgtg aagttccgcc aataccgaaa ccagatggct
     3721 gcagttgcgc aacagtcggt gttgacagtt cagacagcaa tgctcttctt gtcgccgctc
     3781 ttgcagttct tgccgcttta tccgcattgc ttcttagaaa aagaatcgca taaataatct
     3841 tttgatcaga atttgacctg tcttgacttt ttaacagtca aacattcctt attatattgc
     3901 ttaaaacata catataatta actattgaca tattgctatg tctttagtaa tattgttaaa
     3961 gatgttcatg gaggtgtgtc atgaaaaata tcacgatcgg tcttgatgcg aggacttttt
     4021 ttcttatcaa gcaggcgaat cctaaaaacc tgagcaaatt tatccgcgat gcgattgcag
     4081 aaaaagcgca ggcggaactg aaccgtcaga tagatgatta ttgtgccaat ttaaagagca
     4141 gtatggagga tttcgatgga ttcaacagcg aatcagaaga aaactggcca aattaagaga
     4201 ggtgctatag ttattgtcca gtttaatcct tcagtcggat cagagacaaa aaacatccat
     4261 tttgcagtag tggtttccat tgatcagctt aacagccgct cgccgcttat ttctgttgtt
     4321 ccgatgacta caaaagtgga tagattcttt cctgcatttg aagtcatgat caatgaaacc
     4381 ggaaaacctg ccaaagctga ttgtgcgcaa gtcagaacga tcgccaagga acgggtcgtt
     4441 gaaatcaaag gaacagtttc ggaagaaaca ctcatcgaaa tcggtaataa gatcaaaatg
     4501 gctttaggtt tagattgatt tttaattaat cgtctatctt acagaccctt ttttcgtgtc
     4561 ttcccccttc aaaaggtgta aga
//
LOCUS       DAOOYB010000234         4561 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_22176_length_4561_cov_3.499334, whole genome shotgun sequence.
ACCESSION   DAOOYB010000234 DAOOYB010000000
VERSION     DAOOYB010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4561
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22176_length_4561_cov_3.499334"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1152)
                     /locus_tag="PL056_11265"
     CDS             complement(<1..1152)
                     /locus_tag="PL056_11265"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPM47638.1"
                     /translation="MRKNFPLLSVISNYSMLYGPHTVKELLSTASDRGFSTAAITDIN
                     NVYGIHEFINEAAVTGITPLFGSKVSVLNDSAVILARNREGFSSLCRIITEINSKSDN
                     SLKRILLNFQKGITVLARSESLLDRAFECPVYAAMSTPFDIIAKKAHEKGFKAAAVPE
                     AVFINKDAQNTHQLLRAIGLNKNFRDIKKEELAPETSFMPDEETIMQFSDMSPESVKN
                     SINIAESIELHSLFNGFIFPSYKGQTVAESTVELRERTFAGALKRYTDISDTVRNRLN
                     YEIDIITSMGFAAYFLIVADIVSLSSGSCGRGSGAASAVAYSLGITNIDPIKHNLYFE
                     RFLNPERKDPPDIDLDFAWDERGAIIEKVMETFGRNNCAMVCEHLKFRKR"
     gene            complement(1149..2321)
                     /locus_tag="PL056_11270"
     CDS             complement(1149..2321)
                     /locus_tag="PL056_11270"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47639.1"
                     /translation="MKTTIVRVSVSSFAAGIAVASDTSLKGKPFVISSASNLRAVVLD
                     LSSEAQKDGITKGMSVKKASRIIPSLRIIPPSPELYKKADHIISDISGKFSPSIEFGS
                     HGRFFIDISGTSKLWGSAIDTAAKIMTEIRNSIYLDACVGTASSKTVSEIASSVAHPY
                     GLISVRHGSEKHFLAPQPVPLMPGIGPVISERLSIVGINLMGELAGISDRHCIKLLGS
                     GGIKFKEKASGIDTEPLFTEHARKPSIRSSIINQTDPESLEEAMMILLRICEDTGFQM
                     RELDLSAMKVSLNVAFSDGLVRSGSLESTGPLWSDMEIFHFASLILRKIKEEKRTKIR
                     ILTLDLSKLSTSHYQVPLFTHPSVKAEHRLQETMDHLKKRFGMDSVRKALALAAPE"
     gene            complement(2318..3097)
                     /locus_tag="PL056_11275"
     CDS             complement(2318..3097)
                     /locus_tag="PL056_11275"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HPM47640.1"
                     /translation="MNIHIGTSGFDYDDWSGVLYGPDTKRKDRLKIYAQNFSTLELNY
                     TYYSMPDERNIAAMIEVTEGNLSFSVKANKVLTHTPGNIEENAVLFKKALHCLSEKRL
                     LSAVLFQFPYSFHYTPENRKHLSTLLNIFGELPCCVEFRNDEWSGQSVISGLRKRGVA
                     LCSTDMPDLPGLSPGFDLQTADFGYLRLHGRNRDSWWSGDNVTRYDYNYTDREIESVA
                     VRVSNLALKNDNVFVFFNNHRKGNAVKNAFKLQERLKSVQP"
     gene            complement(3238..3426)
                     /locus_tag="PL056_11280"
     CDS             complement(3238..3426)
                     /locus_tag="PL056_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47641.1"
                     /translation="MKKKIRKATVELRIRVDLELQEGMTLDVAVNEMDYDFSCPEGEG
                     AEVTGTEMIDWNLIEGKC"
     gene            complement(3395..3742)
                     /locus_tag="PL056_11285"
     CDS             complement(3395..3742)
                     /locus_tag="PL056_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47642.1"
                     /translation="MIQWFFALLLLSGITLILGAKFWEFMQHCSLTVSIIAGAVIVLF
                     SLAAVSGLPSGVGVIALVVAVATAGAVIINLFTASERCDSPDPAALSGSTESTDGKVT
                     EGGNEKENPQSDG"
     gene            complement(3813..4130)
                     /locus_tag="PL056_11290"
     CDS             complement(3813..4130)
                     /locus_tag="PL056_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47643.1"
                     /translation="MANPTEITFAGNTGRYYSCWYLKDLQKKDDAAFRAIRKDGEIWH
                     TVYHHESDFSEPVTIRKGCGLVNRMGFIIAPENFLPGEEIYIAQAKYRKLCDDIREAE
                     MQS"
     gene            complement(4123..>4561)
                     /locus_tag="PL056_11295"
     CDS             complement(4123..>4561)
                     /locus_tag="PL056_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47644.1"
                     /translation="TMNRKRLNEFRAEAEELINCGNSKEKSRGYGMVAVLDALEEELS
                     LSFPDCTIAILTSGNPADACDPVIERAVFAGSQETINNSVRDQLIEWAETYFDLSEKQ
                     LKDFREDPDYCSSDSECTGDYTVIVEHNCRFVNLSTDNGEQNG"
BASE COUNT         1181 a         1075 c          996 g         1309 t
ORIGIN      
        1 tcttttgcgg aatttaagat gttcacacac catcgcacag ttattacgcc cgaaagtctc
       61 catcactttc tcaattatcg cacccctttc atcccaggca aaatcaagat cgatatcggg
      121 cgggtctttc ctttcagggt tgaggaacct ttcaaaatat aaattgtgct ttattggatc
      181 aatgtttgtt ataccgagtg aatacgcaac agccgaagcg gcgccgctcc ctcttccgca
      241 tgaaccggaa ctaagtgaga caatatccgc cactatcaga aaataagccg caaaacccat
      301 tgaagttatt atatcaattt catagttgag tctgttcctg accgtatcac tgatatctgt
      361 gtatcttttc aaagctcctg caaaagttct ttctcttaat tcaacagtgg attcagcaac
      421 ggtctgacct ttgtatgaag ggaatatgaa cccgttaaaa agcgaatgaa gttcaatact
      481 ttcagcaata tttatactgt ttttaacact ttcaggcgac atgtctgaaa actgcattat
      541 cgtttcttca tcaggcatga atgaagtttc aggagcgagc tcttcttttt taatatctct
      601 gaaattttta ttcagtccga tagcccgcag aagctgatga gtgttctgag catctttatt
      661 tatgaataca gcttcaggga cagccgcagc cttaaacccc ttttcatgag ctttttttgc
      721 aattatgtca aacggtgttg acatcgcggc atataccgga cattcaaaag cacggtcaag
      781 cagtgattca gatcttgcaa gaacggttat ccctttctga aaatttagta aaatacgctt
      841 taacgaatta tcagattttg aatttatttc agtgattatc cggcatagag aggagaatcc
      901 ttccctgtta cgggcaagga tcacagcgga atcattcagt actgatactt ttgagccgaa
      961 cagcggagta atccctgtaa cagccgcttc gtttatgaat tcatgaatgc cgtagacatt
     1021 attgatgtct gtgatggcgg cagttgaaaa tcccctgtct gaagccgtgg aaagaagctc
     1081 ttttacagtg tgcggaccgt aaagcattga atagtttgaa attactgata agagggggaa
     1141 gttctttctc attccggtgc cgcgagagcc agtgcttttc tcactgaatc cataccgaat
     1201 cttttcttaa ggtggtccat tgtctcctgc aggcggtgtt ccgctttaac ggatggatgt
     1261 gtgaacaaag gcacctgata gtgacttgtg gaaagttttg agagatcaag ggtaagtatc
     1321 cgtattttcg ttcttttttc ctctttaatc tttctgagta tcagggacgc gaagtgaaat
     1381 atctccatat cgctccacag cgggccggta ctttccagtg aaccgcttct gacaagaccg
     1441 tcactgaatg caacattcaa agacaccttc atcgctgaca ggtcaagttc ccgcatctgg
     1501 aaaccggtat cttcacatat tctcaggagt atcatcatcg cttcttcaag agattcagga
     1561 tcagtctggt ttataatgga agatctgatc gaaggtttcc gggcatgttc cgtaaaaaga
     1621 ggttcagtat cgatgccgga tgctttttcc ttgaacttta taccaccgct gccgagcagt
     1681 tttatacagt gtctgtcaga tattccggca agttcaccca tcaggtttat accgactatc
     1741 gacagtcttt cgcttatcac gggaccgatc cccggcataa gtggaacggg ctgaggggcg
     1801 agaaaatgtt tttcagaacc gtgtcttacc gatatcagcc cgtaaggatg tgcaacggat
     1861 gaggctattt ccgacactgt ctttgaagag gcggttccga cacatgcgtc aaggtatatt
     1921 gaattcctta tttctgtcat tatcttcgcc gcggtatcta tcgcactgcc ccacagcttt
     1981 gatgtccctg atatatctat gaaaaacctg ccgtgacttc caaattcaat ggaaggggag
     2041 aactttcccg atatgtctga aatgatatga tcggcttttt tataaagttc aggtgaaggg
     2101 ggaattattc tgagcgaagg tattatcctt gatgcctttt tcacggacat tccctttgtt
     2161 atcccgtctt tctgagcttc agaggaaaga tcaagcacca cagctcttaa gtttgatgct
     2221 gaagatatta caaacggttt acctttgagc gaggtatcgg atgcgacggc tatccccgcc
     2281 gcaaatgaag atactgatac ccgaactatt gtggtcttca tggctgtaca cttttaagcc
     2341 tctcctgtaa tttgaaggca tttttcacgg cattcccttt tctgtggttg ttgaagaaca
     2401 caaagacatt gtcgttttta agagccagat tactgacacg gactgctact gattcgatct
     2461 ccctgtcggt ataattatag tcatatctgg taacattgtc tccgctccac caggaatctc
     2521 tgttccttcc gtgaagccgc agatacccga aatcagcggt ctgcaggtca aaacccggtg
     2581 aaagaccggg aagatcgggc atgtcggtgg aacagagtgc gacaccccgt ttccttagac
     2641 ctgatataac gctctgacct gaccattcat cgttcctgaa ctcaacacag cacggcagtt
     2701 caccgaatat attgagaaga gtggaaaggt gttttctgtt ttcaggggta tagtggaaag
     2761 aataagggaa ctgaaagaga acagctgaaa gcagcctttt ttctgatagg cagtgcagtg
     2821 cttttttgaa cagaacggcg ttttcttcta tattcccggg agtatgggtc agaactttgt
     2881 tcgccttgac cgaaaatgaa agattccctt cagttacttc tatcatcgct gcgatgttcc
     2941 tttcatccgg catcgagtag taagtgtagt tcagttcaag tgttgagaag ttctgtgcat
     3001 agatcttaag ccggtctttt ctctttgtgt caggaccata aagcacaccg ctccaatcat
     3061 cataatcaaa tccggaagtc ccgatatgta tattcatttc aacctcgaaa acaggtacag
     3121 acttttcatt atacctaaca cattaaaggt gtcaatgatt tttcgtgcac gtaaaaaaag
     3181 gagccaccat acagcagctc ccttaaatca tcccggcata aagcacaacc ggcaatttca
     3241 acacttacct tcaataaggt tccagtcaat catctcagtg ccggtcactt cagctccttc
     3301 cccttcaggg catgaaaagt catagtccat ttcattgact gccacatcaa gggtcatgcc
     3361 ttcctgaagt tcaaggtcaa cccggatccg gagttcaacc gtcgctttgc ggattttctt
     3421 tttcatttcc accctccgtc acttttccat cggtgctttc agtgctcccg ctcaatgccg
     3481 cgggatccgg agagtcgcac ctttcagaag cggtaaacag gttgataata actgctccgg
     3541 ctgtcgcaac tgcaacaaca agggctatga ccccgacacc tgaaggcaga ccggatactg
     3601 cggccagaga gaaaagaacg ataactgctc cggcgataat cgataccgtc agactgcagt
     3661 gctgcatgaa ctcccagaac ttggcaccga gaataagtgt tatcccggat aaaagtaaaa
     3721 gtgcaaaaaa ccactggatc atttctgtgt cctccgttaa gttgttaaat tcataagata
     3781 aaatccctct ttccgtgagg ggctgatgtt gactaggact gcatctccgc ttcccggatg
     3841 tcgtcgcaga gcttacggta ctttgcctga gcgatgtaga tctcttcacc ggggaggaag
     3901 ttctcaggtg cgatgataaa gcccatacgg ttcaccagtc cgcaaccttt ccttattgtg
     3961 accggctcgg aaaagtcgga ttcatggtga tagacggtgt gccagatctc gccatccttc
     4021 cgtatagccc tgaaggcggc atcatccttt ttctgaagat ccttgagata ccagcaggaa
     4081 tagtatctgc cggtattccc tgcaaaagtg atttctgtgg ggttagccat tctgttctcc
     4141 attgtctgtt gataagttta caaaccggca gttgtgctcg acaatgacag tgtagtcgcc
     4201 ggtgcattca ctgtcggaac tgcagtagtc gggatcttcc ctgaagtcct tgagctgttt
     4261 ctcggaaagg tcgaaataag tctccgccca ctcaatcagc tgatctctca cagaattgtt
     4321 gatagtttcc tgagaaccgg caaagactgc cctttcaatg accgggtcac aggcatcggc
     4381 aggattaccg ctggtaagga tggcgatagt gcagtcggga aaggaaaggg aaagttcttc
     4441 ctccaacgca tcaagcacag ccaccatgcc gtaaccacgg ctcttctctt tactgttccc
     4501 gcagtttata agctcctccg cttctgcacg aaactcattc agtctttttc tgttcatagt
     4561 t
//
LOCUS       DAOOYB010000235         4503 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_22557_length_4503_cov_13.559353, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000235 DAOOYB010000000
VERSION     DAOOYB010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4503
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22557_length_4503_cov_13.559353"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /gene="pth"
                     /locus_tag="PL056_11300"
     CDS             complement(<1..351)
                     /gene="pth"
                     /locus_tag="PL056_11300"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HPM47645.1"
                     /translation="MLVVFLGNPGSQYKKTRHNAGFMLADEIEFLKNAVWNEKFSGQY
                     AKAEVSGVSHHFLKPMTFMNVSGKSVAAAIAFFKIKPVEVLVVHDDIETEFETVSIKK
                     GGGLAGHNGLKSIAN"
     gene            487..2256
                     /gene="recD"
                     /locus_tag="PL056_11305"
     CDS             487..2256
                     /gene="recD"
                     /locus_tag="PL056_11305"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HPM47646.1"
                     /translation="MANIIKRLLSSGLIDDLDFHFAQFTGRIEKRGFSSDEFEILSVS
                     AALISRQINCGHICLSENDVENLRNETENGIEIIPQWADIINVMSKSSSCSNGSTLTP
                     VVFDGARLYLYRYWQYENRLAKKIIEMSKEPSRYIEKYQELAKTITLLFGEEGKTNGQ
                     LDAALSAVRNRFSVITGGPGTGKTTAIAKILAAIFRLFPDESVVLAAPTGKAASRMNE
                     ALKNAVSFAKNSTDDEIKLKIQGLNGCSIHRLLGWTRTPGVFVHNNENPIDAGIVIID
                     EASMIDLSLMSKLVDSIHSGTSLLLLGDKDQLTSVEAGNVLGDICTASNKGVFKDSNV
                     SVLTKSYRFGKSAFIGELASEVNLGSDTSILKEIFNKCNDGSIKFIDSKISSSMEVVE
                     EAIIRNYSSLFKDSNALEIIATLEKFRILCGSKVGNGGVAQINRFAESVMIKNGSIKS
                     HGEQWYHGRPVMVTENNYVLELFNGDCGVILEKDNEKRGYFQSNDGTVRSFPVSMLPS
                     VETVYAMTVHKSQGSEYDSVMVILPEKEMAVLTKELIYTAVTRSRKDLTIIGKEEILS
                     TAVKRSAARNSGLADALIFLSKQ"
     gene            2370..3272
                     /locus_tag="PL056_11310"
     CDS             2370..3272
                     /locus_tag="PL056_11310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47647.1"
                     /translation="MKFKVFIFVLFASFIAEAGVCIQINSENDTLMPNEQNAAKFMAE
                     KEFQNEGAVVDGTCTETYTLTHLLLGSSIIVVMEKGGSRQSVVIEGKDELAKAYNQLA
                     RYFLKGISASYKNTTTENVIGTQAEPPRKIGSELNFMMMLGYGIQPTPDIGGGINWTL
                     GLRYDLRYMFLDFEFGCGLWDAHGPEYLEFNLVGFKGAYYFSEKDPSSWYIGAGIRAG
                     FYFLSDNDYDYDEATEFSGSGIISIGHEWLRNSSKRFMVQFDAKFPFHSMKLENETYN
                     NGQTVRETKRFYFPTLSINLGVGL"
     gene            complement(3281..3883)
                     /locus_tag="PL056_11315"
     CDS             complement(3281..3883)
                     /locus_tag="PL056_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47648.1"
                     /translation="MNKIILLFWALMIFMFFAGCKAPACTDLSWGETSQVEESVELKY
                     SAGNKCENISDYWDVDINEIISKIEGAELVISKCVESVSDQEVECEEFIPEKIGFFEI
                     TGCRFETPPDSNTKCKASPLIIFSESDSVFKKISWSFNLENEGDVVEISSLFDEKRLE
                     SLPFSLSDKSILMIFKWTEHSNDNIEKELEMFAEYSVTYK"
     gene            complement(3922..>4503)
                     /locus_tag="PL056_11320"
     CDS             complement(3922..>4503)
                     /locus_tag="PL056_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HPM47649.1"
                     /translation="GLNAFTMSPQKWINATNATGFVVKAGRYGGTYAHKDIAFKFASY
                     ISVEFELYIVKEFQRLKEEEQKQLGWSAKRELAKINYHIHTDAIKKNLIPKELTSAQI
                     SVVYATEADVLNVALFGMTAKQWKEANPELIGNMRDHALINELICLSNLENLNALFIA
                     EGMSQTERLIKLNKIAIEQMIILLEAENRKLLK"
BASE COUNT         1383 a          926 c          857 g         1337 t
ORIGIN      
        1 atttgctatt gatttcaatc cgttatgacc tgcaagcccg ccacctttct ttattgagac
       61 tgtttcaaat tctgtttcaa tatcatcatg gacaacaagg acttcaaccg gctttatctt
      121 gaaaaaagct attgccgccg caacactttt tccactcaca ttcatgaaag tcatcggctt
      181 aagaaaatgg tgcgaaacac cgcttacttc agcttttgca tattgaccgc tgaacttttc
      241 gttccatacc gcatttttta agaattcaat ctcatcagcc aacatgaaac ctgcgttgtg
      301 ccttgttttt ttatattgac tccccggatt tccaagaaaa acaacaagca tcattttctc
      361 cttgaatcaa ctgtgcatta tattacccgt tcccttcata ttttcaagct gcttgattat
      421 tatctgtttt taggttaatc tccatctgat atgacattct ggttcctgct aattaacgag
      481 gttgaattgg ctaatattat caagcggttg cttagttcag ggttaataga cgaccttgac
      541 tttcactttg cacaatttac aggcagaatt gaaaaaagag gattttcttc agacgaattt
      601 gagatcttgt cggtaagtgc agctctgata agccgccaga taaactgcgg tcacatctgt
      661 ctttctgaaa atgatgtaga aaatttaaga aatgaaactg agaacggaat tgaaataatt
      721 ccgcaatggg ctgatattat taatgttatg tcaaaaagct caagctgttc aaatggaagt
      781 actttgaccc ctgtcgtttt tgacggtgca agattatatc tctacagata ctggcagtat
      841 gaaaacagat tggcaaaaaa gatcattgaa atgtcaaagg aaccgagcag atatattgaa
      901 aaatatcaag agcttgcaaa aactatcact ttgcttttcg gtgaagaggg aaagacaaac
      961 ggacaacttg acgcggcact ttcagcggtc agaaaccgtt tttcagtaat aacaggcggg
     1021 ccgggaacag gaaaaacaac tgccatcgca aaaatacttg ccgcaatatt cagactgttt
     1081 cctgatgaat cggttgtcct tgccgctccg acaggaaaag cggcatcaag aatgaacgaa
     1141 gcacttaaaa acgcagtgtc atttgccaaa aactcaactg atgatgaaat aaagctgaaa
     1201 attcaaggac ttaacgggtg ttccatccac agacttcttg gatggacaag aactccggga
     1261 gtatttgtcc ataacaatga aaatcctatc gatgccggaa tcgtgataat cgatgaagca
     1321 agcatgatcg atctgtcgct tatgtcaaaa cttgtcgatt caatacattc cggcacatcg
     1381 ttactgcttc ttggagacaa agatcagctt acaagtgtgg aagccggaaa tgttctggga
     1441 gatatatgca ccgcctcgaa taaaggtgtt ttcaaagata gcaatgtgtc ggtccttacc
     1501 aaaagctacc gtttcggaaa aagtgctttc atcggagagc tcgcttcaga agtaaatctc
     1561 gggtcagaca cctcaattct aaaagaaatt ttcaataaat gtaatgacgg ttccataaag
     1621 ttcatagact cgaaaatttc atcttccatg gaggtcgttg aagaggcaat tatccggaat
     1681 tattcatctt tatttaaaga ttccaacgct ttggaaatta tagccaccct tgaaaaattc
     1741 agaatactct gtggctcaaa ggtcggaaac ggcggagtcg cacagataaa ccggtttgcc
     1801 gaaagcgtca tgatcaaaaa cggatcaatt aaatctcatg gtgaacagtg gtatcacgga
     1861 agaccggtca tggtgacgga aaacaattat gttcttgaac tttttaacgg agattgcggc
     1921 gtaatccttg aaaaagacaa tgaaaaaaga ggttatttcc agtcaaatga cggaaccgtg
     1981 agatctttcc ccgtctcaat gctcccttct gttgaaacgg tatatgccat gaccgttcac
     2041 aaatctcagg ggagtgaata tgactccgta atggtcatac ttcctgaaaa agagatggcg
     2101 gtgctgacaa aagagctcat atatacagct gtcacccgtt caagaaaaga tctcactatc
     2161 atcggcaaag aagagatcct gtcaactgcc gtaaaaagat ctgccgcaag gaattccgga
     2221 cttgcagatg ccttaatttt tctttcaaaa cagtaagttg tgaatataat cgcttttgtt
     2281 tcagctgttt tataataaaa tctgaacctt actaaaacta aagctgtgac cgaatcgttt
     2341 tgttttatat ttggaatgga ggatatgtca tgaagttcaa ggtttttatt ttcgtccttt
     2401 ttgcaagttt cattgcagaa gccggcgtat gtatccagat caattctgag aacgacacgc
     2461 tgatgcccaa tgagcagaat gccgcaaaat tcatggcgga aaaagagttt cagaacgaag
     2521 gcgcagtagt tgacggaaca tgtactgaaa cttatacatt gacccatctt cttcttggca
     2581 gcagtattat tgtcgtcatg gaaaaagggg gcagcagaca gagcgttgtt attgaaggaa
     2641 aagatgaact cgcaaaagct tacaaccagc ttgcaagata ttttttaaaa ggaatttcag
     2701 caagttacaa aaacaccaca actgaaaatg tcatcggcac acaagccgaa ccgccaagaa
     2761 aaataggaag tgaacttaat ttcatgatga tgctcggtta cggaattcag ccgacacctg
     2821 acatcggagg cggcatcaac tggacacttg gattaagata tgatctccgc tacatgttcc
     2881 ttgattttga attcggctgc ggactctggg atgcacacgg tccggagtat cttgaattca
     2941 accttgtcgg ctttaaaggg gcttattatt tcagcgaaaa ggatccgtca agctggtata
     3001 tcggtgcagg gatcagggcc ggcttctatt ttctttcgga taacgactat gattacgatg
     3061 aagccacaga attcagcgga tcagggatca tcagcatcgg acacgaatgg ctcagaaact
     3121 cttcaaaaag gttcatggtg cagtttgatg caaaattccc attccattca atgaaactcg
     3181 aaaatgaaac ttataacaac ggacaaaccg tcagggaaac aaaaagattt tatttcccga
     3241 ccctttcaat caatctcggt gtaggacttt aaaagattct ttatttgtaa gttactgaat
     3301 attctgcaaa catttccaat tctttttcaa tattatcatt tgaatgttca gtccatttaa
     3361 aaatcatcaa tatggacttg tcagacaaag aaaaaggaag agattccaat cttttctcat
     3421 caaataaaga tgagatttca acaacatcac cttcattttc caaattaaat gaccaactta
     3481 ttttcttaaa aacggagtcg ctttcagaaa aaattatcaa tgggcttgct ttgcattttg
     3541 tgtttgaatc aggaggtgtt tcaaaacggc atccggtaat ctcaaaaaac ccgattttct
     3601 ctggaataaa ttcttcacat tctacttcct gatctgacac ggactcaaca catttactga
     3661 ttacaagctc agcaccctca attttagata ttatttcatt tatatccaca tcccagtaat
     3721 cactgatgtt ttcgcatttg ttgcctgcgc tgtatttaag ttctacagat tcttcaacct
     3781 gcgatgtttc accccatgac aagtcagtgc atgccggtgc tttgcagccc gcaaaaaaca
     3841 tgaaaatcat caatgcccaa aacagcaata ttattttatt catctttatc ctccaagctt
     3901 attgttttaa attccctttt tttatttcaa cagcttcctg ttttcggctt ccagaagaat
     3961 gatcatctgc tcaattgcga tcttatttaa tttaatcagt ctttctgtct gcgacattcc
     4021 ttccgcaatg aataaagcgt tcaggttttc aagatttgaa aggcatatca actcgtttat
     4081 caaggcatga tccctcatat tcccgattag ctcagggttg gcctctttcc attgttttgc
     4141 cgtcattccg aaaagagcaa cattaagcac atcagcttcc gtcgcataaa caactgaaat
     4201 ttgagcagat gtgagttctt tcggaatcag gttctttttt attgcatctg tgtgaatgtg
     4261 gtaattaatc tttgcaagtt ctctttttgc cgaccagccg agctgtttct gctcttcttc
     4321 tttaagacgc tgaaattctt tcactatata caattcaaat tcaacagaaa tgtaacttgc
     4381 aaatttaaag gcaatatctt tatgagcata ggttccacca tagcgacctg cctttactac
     4441 gaaacctgtt gcatttgtgg catttatcca tttttgagga gacatcgtaa acgcattcaa
     4501 tcc
//
LOCUS       DAOOYB010000236         4472 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_22795_length_4472_cov_6.299977, whole genome shotgun sequence.
ACCESSION   DAOOYB010000236 DAOOYB010000000
VERSION     DAOOYB010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4472
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22795_length_4472_cov_6.299977"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>4472)
                     /locus_tag="PL056_11325"
     CDS             complement(<1..>4472)
                     /locus_tag="PL056_11325"
                     /inference="COORDINATES: protein motif:HMM:NF024807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HPM47650.1"
                     /translation="SEELTGNPTVTLTKNGGGTVTPNNTEVQGLSYIYTYPTLADGTY
                     SFTADAADLAGHNAAQVSITSIISDSSVPNVSGLVDVSPTRIKPSATSTITFTASETM
                     NDTGDIVEVKVGNGTANCTTANGLNYSCTYTGSGTGSDSIETISITLTDSSANTSTSQ
                     HGSIYVDRTAPGMVSALLTPENAKEDTKISVSVIFDEPVKDLSMSDDGLGLVCSTGGD
                     PLRYTCEYIVKITDVEKEYAVEISANDIAGNPLTDGVLGTVYVDMTLPQIEVNTCSVT
                     TRRAITKSGMAAATTGDVVEITLGMNVESEIATEITLGNRLLTQDCETPADNCFAYSV
                     SGSDSEGYKFVGIEAFDNAGNYYSETVDIENCSSVFDFTGPILATAVISRIPDYVPAR
                     DHSTKTLNFSLTDPFTDEAVIAQLNLFADEELDSETIEITGFDFGEPLEVIDNYASFE
                     RMLDNGISEGTKNLSVLWQDILGNSATRAVNWKMFIDKTEPNPSVIDMKKVLYTRKPW
                     GTDDTGGVPKFSVAGETGAITDPDISTIIAYNELGSIIGNTTITDGSFSIPMLNGGDL
                     PKIYLNPIKKSGVKSAGTGALVTEIKWHATMGGKVPGSTLINPHRFTSTSKFKYSVAQ
                     IDEMSTEPSDNDYQNLKTHDANIFKTFSDTVWHQIGNTSFPAPRYLASLVWSANTGKF
                     ILFGGAKGEHLDSVYQDMWEYDPDQRIWRSADTTGLKPQGRLAHSAAINNKNGNMLIF
                     GGAFAKDVFANDTWIFNTETRSWRQIIQNNGPSGRMAASIVYDSDRNRFVMFGGMSLE
                     SGLNELWEFIVEEEIWVQVTVTGDAPSPRTGASMAYDPVRKKIILFGGGDFNFALQDT
                     WEYDTQNIQWENRSGDDVTPPGMREFAVMGYDSNTGTAVLYGGDSGENGTVYSDIWKY
                     EPISGTWSNISFSNGPESRMWGTGSFAENRSELAIFGGLLPASESNDLWTWKNNLAQW
                     ERYAPMIIQPSSRSDYGFVSDDLGNIYLFSGQDSINEFNDLWRFNVSNEQWEDISVAT
                     NPSYRFAYLMTYVSAGQKLIMFGGIDGENDFQSTWEYNIAAKQWYSRTMVGNRPPAGW
                     QHGRMIYDNDRNRVLWLDDEEFWEYNPATYVWTKINPAGILPPLRSQFGFAYDIHRKK
                     AVIFGGEGETLPLNDVWEYDAVNNSWEERIPVGDKPPLWDSLTMVYDKNIRKMVVFGG
                     NQGFLETMTYGNTNDLWLWDGNSGTWEKETFNSDVPMKRDRHQMTYDVLSQRSYLIGG
                     EEDAHGNFNDLWSFDSGSGSHPGHVAWINTSYIGLTYENLYRVEFKFQVGGTGYAGTN
                     CGAINGTKMLLWNTDYRSGSWMQVATNTADSSTPQTLEFFTSDPDLMANLFFGNERSV
                     NIAVVPLSPSGCGSEKGSVSTDYAEVMLHYSLDSESVQPDPGIDNLYYVSTDALSWED
                     ARLACHELNGDLVSINSELEQYYIERMISTSHDYWIGATDKTKEGDWRWVDGTK"
BASE COUNT         1138 a         1055 c          847 g         1432 t
ORIGIN      
        1 atttcgtgcc gtccacccag cgccagtcac cttcttttgt tttatcagtc gcaccgatcc
       61 agtaatcgtg agaggtggaa atcattcgtt cgatgtaata ttgctcaagt tcgctgttga
      121 tggagaccag atcaccattc aattcatgac atgcaagtct tgcgtcttcc cagcttaaag
      181 catctgtact tacataataa aggttgtcta ttcccgggtc gggttgtaca gattcgctat
      241 ccaatgaata gtgcaacata acttcagcat aatcagttga gacactgcct ttttcagatc
      301 cacatccgct tggagataaa ggaacaacag caatattgac agatctttca ttgccgaaga
      361 aaaggtttgc cattaaatca gggtcagaag taaaaaattc cagtgtttga ggcgttgaag
      421 aatctgcagt attagttgcc acttgcatcc atgagccact gcgatagtcg gtgttccaca
      481 acaacatctt cgtgccgttt attgcaccgc agttcgttcc ggcataacct gttccgccaa
      541 cttggaattt gaattcaacg cggtaaagat tttcgtatgt taatccaatg taagaagtgt
      601 ttatccacgc aacatgtccg ggatgtgatc ctgatccgct gtcaaatgac cacaagtcgt
      661 tgaagtttcc atgtgcatct tcttctccgc caataagata tgatctttgt gataatacat
      721 cgtatgtcat ctggtgacga tctctcttca tcggaacatc actgttaaag gtttcttttt
      781 cccatgtgcc ggaattgccg tcccacaacc aaaggtcgtt agtatttcca taagtcatcg
      841 tttctaagaa tccttgattc cctccaaaga ccaccatttt ccttatattt ttatcataca
      901 ccattgttaa cgagtcccat aatgggggtt tgtctccgac aggtattctt tcttcccatg
      961 aattattcac tgcatcatat tcccaaacat cattcaaagg aagtgtttct ccttcaccgc
     1021 cgaagattac agcttttttt ctatgaatat catacgcaaa accgaattgg cttcttaatg
     1081 gcggaaggat tcctgcagga ttaatcttgg tccaaacata ggttgcggga ttatactccc
     1141 agaattcttc atcatccaac cacaaaactc tgttacggtc attgtcatat atcattcttc
     1201 cgtgctgcca tccagccggt ggtctgttac cgaccattgt tctgctatac cattgtttcg
     1261 ccgcaatatt gtattcccag gtgctttgga aatcgttttc tccgtcaatt cccccgaaca
     1321 taataagttt ttgcccagca gaaacataag tcatcagata tgcaaaacga taagacgggt
     1381 ttgttgcgac agaaatatct tcccactgtt cgttagatac attaaatctc cacaaatcat
     1441 tgaactcatt aatactgtct tgtccgctga ataaataaat atttcctaaa tcatctgaaa
     1501 caaaaccata gtcagatctg ctgcttggct gtattatcat gggagcatat ctttcccact
     1561 gtgccagatt atttttccaa gtccataaat cgtttgattc actcgcaggc aaaagacctc
     1621 caaatattgc cagctcactt ctattttcgg caaaggaacc tgtcccccac attcttgatt
     1681 caggaccatt agaaaaagaa atgttgctcc atgttcctga aatcggctcg tatttccata
     1741 tatcagaata aacagttccg ttttcgccac tgtcaccgcc ataaagaact gctgtaccgg
     1801 tgtttgaatc atatcccatc acggcaaact ccctcatgcc gggaggagta acatcatcac
     1861 cacttctgtt ttcccactgt atattctgcg tgtcatattc ccatgtgtcc tgtaaagcaa
     1921 aattaaagtc tcctcctccg aaaagaataa ttttttttct tacgggatca tacgccattg
     1981 atgccccggt tcttggactt ggtgcatcac ctgtcactgt tacttgtacc catatttctt
     2041 cttcaacaat aaattcccac aattcgttta atccggattc cagtgacata cctccaaaca
     2101 ttacaaaacg gtttctgtca ctatcataaa cgattgaagc tgccattctg ccggaaggtc
     2161 cgttattttg aataatttgt ctccagcttc tggtttcagt attaaatatc catgtgtcat
     2221 tcgcaaagac atctttagca aacgcacctc cgaaaatcag catattccca tttttattat
     2281 ttattgcagc ggaatgcgca agacgacctt gcggctttaa cccagttgtg tctgctgatc
     2341 gccagatcct ttggtccgga tcatattccc acatatcctg ataaactgaa tccaaatgtt
     2401 cccctttagc tccgccgaaa aggataaatt tcccggtatt tgcactccac actaatgaag
     2461 ccaagtacct cggggcgggg aaagaggtgt ttcctatctg atgccatact gtgtctgaaa
     2521 aagttttgaa aatattggca tcatgtgttt ttaaattctg ataatcattg tcagatggtt
     2581 cagttgacat ttcatcaatt tgagcaactg aatatttgaa tttacttgtt gaagtaaatc
     2641 tgtgtggatt tatcagtgtg cttcccggta ccttgcctcc catcgttgca tgccatttta
     2701 tttcggtgac aagagcacct gtccctgctg atttaactcc gcttttcttt atcgggttga
     2761 gataaatctt ggggagatcg cccccattca gcatgggtat gctgaacgat ccatctgtta
     2821 tggttgtatt tcctattata gagccgagct cgttataagc tataattgtt gaaatgtcag
     2881 gatctgtaat agcccccgtt tcccccgcaa cactgaactt aggaactcct cctgtgtcat
     2941 ccgttcccca tggttttctt gtgtagagaa cttttttcat atcaattaca gacgggtttg
     3001 gctctgtttt gtcaatgaac attttccagt tcactgccct tgttgcactg tttccaagaa
     3061 tatcctgcca caatactgat aaattctttg tcccttcaga gattccgtta tcgagcatcc
     3121 tttcaaacga cgcatagttg tcgatcactt cgagcggttc accgaaatca aagccggtta
     3181 tttcaattgt ttcagaatca agctcctcgt ctgcaaaaag attgagctgt gcaatgaccg
     3241 cttcatctgt aaacggatcg gtcaagctga aatttaatgt ttttgtgctg tgatcccttg
     3301 ccggaacata gtcagggatc cttgaaatta cagcagttgc aaggatcggt ccggtaaaat
     3361 caaaaacaga cgaacagttc tcaatatcaa cagtttctga ataatagttg ccggcattgt
     3421 caaatgcttc aattcctacg aatttatagc cttcgctgtc gcttcctgaa actgaataag
     3481 caaaacagtt gtcagccggt gtttcgcaat cctgtgtcag gagtctgttg ccaagtgtta
     3541 tctccgttgc aatttctgat tcaacattca ttccgagcgt tatttctacc acatctcctg
     3601 ttgtcgccgc cgccattccg ctctttgtaa tcgctcttct tgtggtgact gagcatgtgt
     3661 tgacctctat ctgcggcaat gtcatatcaa cataaactgt tccgagaact ccgtctgtaa
     3721 gagggtttcc ggcgatatca tttgcagata tttcaacagc atattctttt tcaacatcag
     3781 tgatttttac aatgtattcg catgtatatc ttagcggatc gcctcctgtt gaacacacaa
     3841 gcccaagtcc gtcatcactc atactcaaat ctttaaccgg ttcatcaaat attaccgaaa
     3901 cagaaatttt tgtgtcttct ttcgcgtttt caggtgtcag cagagcactt accatgcctg
     3961 gtgcagttct gtcaacatat atcgaaccgt gctgacttgt gcttgtgttg gcactgctgt
     4021 ctgtcagtgt gatgctgatc gtttcaatac tgtcactgcc tgtgcctgaa ccggtatatg
     4081 tgcaggaata gttcagtccg tttgctgttg tgcagtttgc tgtgccgttt cctaccttaa
     4141 cttcaacgat atcacctgta tcgttcatcg tttcactagc ggtaaatgtg atggttgaag
     4201 ttgctgatgg ttttattctt gtcgggctga catcaaccaa tccggaaaca ttcggaactg
     4261 aagagtctga tattatcgat gttattgata cctgcgccgc attgtgtccg gcaaggtctg
     4321 ccgcatcggc tgtgaaactg taagttccgt cagcaagtgt cggatatgtg taaatgtatg
     4381 aaagtccctg aacttcagtg ttatttggag taactgttcc gccaccgttc tttgtgagtg
     4441 ttacagtcgg attaccggtc aactcttcgg at
//
LOCUS       DAOOYB010000237         4434 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_23092_length_4434_cov_5.024206, whole genome shotgun sequence.
ACCESSION   DAOOYB010000237 DAOOYB010000000
VERSION     DAOOYB010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4434
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23092_length_4434_cov_5.024206"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="PL056_11330"
     CDS             <1..642
                     /locus_tag="PL056_11330"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HPM47651.1"
                     /translation="HKPKTSSKRKIALTAEQISLIANYQTDDPKAQFYRDMFMFSFLA
                     CGMNMTDIARLRYSNIEGNEIVFVRKKTEAEENTEEPLQIPITKRMQSIIDTHGIRAV
                     GYDSFIFPILRPEWDEERKFAEVRQLTKMVNQHLKRIAKAVKIKDNVSSYVARHSWAT
                     ISKNTGASTEYISEQLGHSSVLVTKSYLKGFEKATREQQSSKTEDAVYNQKAV"
     gene            642..1388
                     /locus_tag="PL056_11335"
     CDS             642..1388
                     /locus_tag="PL056_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47652.1"
                     /translation="MAESNYKRDIIQEFFYPLKGYQKSPGRSYFIKDEIDLAEKLAQH
                     YNLPLDNILRLISYTEKSLEWALQMEQTFNINDLSGLFHFEARPEPIRKVSFECDNIS
                     FTITNPYLIDRLYQTAHEAMNTTLDKLKQHKDDEFSMNGMLGVYIYQNRIFNEGPKRV
                     SGPIIKSIGTEIIRELMDVEMLSEYKSVCILGAIMSLYKVGLKANPIMTEQEHREDCA
                     KNKIEMKMGEKVISDSYLTYCFGIAKNWHY"
     gene            1981..2337
                     /locus_tag="PL056_11340"
     CDS             1981..2337
                     /locus_tag="PL056_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47653.1"
                     /translation="MHRLILGVVDQPEIEVDHIHHNKLDNRRAMIRTCSRAENSRNRA
                     KGRGYSNYKGVYRENYLWHSQICCDQKVINLGRYRSEVTAAKAYDRAARDLFGDFCNP
                     NFITHEPLPQQMTIQM"
     gene            2358..3908
                     /locus_tag="PL056_11345"
     CDS             2358..3908
                     /locus_tag="PL056_11345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47654.1"
                     /translation="MKKEKVKYNAILTDNLLRLLQELDDNRPPIPAYKRSRMMYLLST
                     IMTHPQDKHPGAWSVLHMNYLRAVVPNAYQYIDYLNEEGVIEVKNHFAGRNSRLYRIA
                     KEYQGPAVFRTITDMALLNRIQEARCEIAKHNSRKYPDLNMMTSRVRIDVSAAHETIE
                     AMYQATEDKRAAEARRTYSLAEVEKIYRGEIFHTVNDTNFRYDTNFTRLPSELVQHLT
                     IEGKPLQELDICNSQIFFSVCLFDPTPEVTRVMRSYLGQKLTIDTKRLQLSDKYDVKR
                     YALLATSGEFYEGMMKLFNLSDRDEVKELCFTVLFSKNTAVRYSKDVRMFRDEFPSVF
                     RMFTFIKSEGHNRLAVLLQRIESDVILNHCVPAIRKQLPDVMFTTKHDSILPAILLVA
                     GDRAGEVARVMTETIEEVTGLKPKIKIKQAQILKSHSATSNTSTPINSNQSTPPLNTF
                     YPIIRTKTLQLVENDIVRWGRLSGKMKRQIKKFKKDNQASVTVVDDKKSASSLAPAGE
                     LIKESCKK"
     gene            3930..4124
                     /locus_tag="PL056_11350"
     CDS             3930..4124
                     /locus_tag="PL056_11350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47655.1"
                     /translation="MTKSHSPHEVKFKSDTDPHFAIVEILKRATGEIKASHYILKSDV
                     PQWLSYFESQGFTIKNSDNG"
BASE COUNT         1470 a          897 c          964 g         1103 t
ORIGIN      
        1 cacaagccta aaacatcatc caaacgcaag atcgcactca cggcagaaca gatcagcctc
       61 attgcgaact atcagaccga cgatcccaaa gcacaatttt accgtgatat gttcatgttt
      121 tcttttctag cctgcggcat gaatatgaca gacatagcac gattgagata ttcaaatatt
      181 gaaggcaatg agatagtgtt tgtccgtaag aaaacagagg cagaagagaa tactgaagag
      241 cctttgcaga ttccgataac aaagcgaatg caatcaatta tagacactca tggtatcagg
      301 gcagtggggt atgattcctt tatatttccg attctccggc cagagtggga tgaagagcga
      361 aagtttgccg aagtgaggca gttgacaaaa atggttaacc agcacctgaa gcgaatagca
      421 aaggcagtga agataaaaga taatgtgtct agctatgtgg ctcgtcactc atgggcgaca
      481 atctcaaaga acaccggagc cagcaccgag tatatcagtg aacagttagg gcactcttct
      541 gtattggtga caaagtcata tctcaaagga tttgaaaagg ccacaaggga acagcagtca
      601 tccaaaactg aagatgcagt ttataatcaa aaggcagtgt gatggcagaa tcaaattata
      661 aaagggacat tatacaggag tttttttatc cgctcaaagg atatcagaaa tctccaggac
      721 gaagttattt cataaaggat gagattgatc ttgccgaaaa acttgcccag cactacaatc
      781 tgccccttga caatattctc agattaatct cctatacaga gaagtcatta gaatgggcat
      841 tgcagatgga gcagacattt aacattaacg atttatccgg cctctttcac tttgaagcaa
      901 gaccggaacc aatcagaaaa gtgagctttg aatgcgataa tatctcattc actatcacta
      961 atccctacct tattgaccgg ctttatcaaa cagcacacga ggcaatgaac acaacactgg
     1021 ataaactgaa acagcataag gatgatgagt tttcaatgaa cggaatgctt ggtgtctata
     1081 tctatcagaa caggatattt aatgaagggc caaagagagt atcagggcct atcataaaat
     1141 caatagggac agaaataatt agggagctta tggatgtaga aatgctgtca gaatataaat
     1201 cggtatgtat tttaggagca ataatgagcc tctataaagt cggattaaaa gccaatccta
     1261 tcatgaccga acaagagcat cgtgaagatt gtgccaagaa taaaatagaa atgaaaatgg
     1321 gggagaaggt aatttctgac agttatctta cttattgctt cggaatagcc aagaattggc
     1381 attactagta tttattctat tcctctattt cttaactgat tgatattcaa ttagggtaaa
     1441 tttgcttaaa attaggcatt tttatcctat gtcattcact tgcaagtgtt tatgataact
     1501 tattgctttg ttatgtcaaa caataaaaaa tgatatgaca gcaataactt ttgaaaattt
     1561 accgcaagct gtaaaagagc tgcacgacaa aatcgattat ctattaggag ccaggcagga
     1621 gaagcctgaa accgattatt taatggaggt ggatcaactt ctccaatatt tgccggatca
     1681 ccctgcaaga caaactgtgt atcagtggat atggaagcgg tccatacctt atgaaaaaca
     1741 cggcaaaaga ttgtatttcc gcagaagtac gattgatcaa tggctcgcta acggcaggga
     1801 actcacccga taactgagat gatcccagat aagaactttg cgacgctgtt gatcacttcc
     1861 aggggggtgg atcatctttg tctctttgac agaagtgaca gtgatttgat tttaaaatac
     1921 aaatggcatc tcaacagtca aggttacgca tcaactcgaa ttgagggtaa gcaggtcatg
     1981 atgcaccgtc tgattctagg agttgttgac cagcctgaga ttgaagttga tcacattcat
     2041 cacaacaagc tcgacaatcg tcgggcaatg atccgcacct gttccagggc agagaactca
     2101 cgcaacagag caaagggaag agggtactca aattacaaag gcgtttatcg tgaaaattac
     2161 ttgtggcata gtcaaatatg ctgcgatcaa aaagtgataa atctaggtcg ttatcggtca
     2221 gaagttacag ccgcaaaggc ttatgatcgt gcagctcgtg atctttttgg cgacttctgc
     2281 aatccgaatt tcataacaca tgagccactg cctcaacaaa tgacaattca gatgtaacaa
     2341 ctcacagttc caccacagtg aaaaaagaaa aggtaaaata caatgcgatc ctcacagata
     2401 atttactccg gctacttcag gagcttgacg ataaccggcc acccatacca gcatacaaaa
     2461 ggtcacgcat gatgtattta ctctccacca taatgaccca cccacaagat aaacacccag
     2521 gagcctggtc tgtacttcac atgaattatc tcagggccgt cgtgcctaac gcataccagt
     2581 atattgatta cttgaatgaa gagggagtga ttgaagtaaa aaatcatttc gcaggccgta
     2641 acagtcgcct gtaccggata gcaaaagaat atcagggtcc ggcagtcttc aggacgatta
     2701 cagacatggc cttacttaat cggattcagg aggcacgatg cgagattgcg aaacacaatt
     2761 cacgcaaata tcctgacttg aacatgatga cttcacgggt cagaatagat gtgtccgcag
     2821 cacatgaaac gattgaagca atgtatcagg ccactgaaga caagcgagct gcggaagcac
     2881 ggagaacata ctccctggcc gaagttgaaa agatatacag gggtgagata tttcacacgg
     2941 tcaatgacac aaacttcaga tatgacacca acttcacccg actgccgtcg gagctggtgc
     3001 agcatctcac tattgagggc aagccgttac aggagctgga catatgcaat tcacagatat
     3061 ttttctctgt ctgtctcttt gatcctacac ctgaagtgac acgggtcatg aggagctatt
     3121 taggccaaaa acttacaatt gatacaaaaa gactgcaatt gtctgacaaa tatgatgtta
     3181 agcggtatgc tctattggca acttcgggcg agttttatga gggtatgatg aagctgttta
     3241 atctctcaga cagagacgag gtaaaagaat tgtgcttcac agtcctgttc agcaagaata
     3301 ccgccgtccg gtattcaaag gatgtgagga tgttcaggga tgaatttccc agtgtcttca
     3361 ggatgttcac ctttattaag tctgaaggtc acaatcgtct cgctgtttta cttcagagaa
     3421 ttgaatcaga tgttatcctg aatcattgtg ttccggcaat tcgcaagcaa ctgcctgatg
     3481 tgatgttcac cacgaagcac gattcaatac ttccagcgat tctacttgtg gccggagaca
     3541 gggccggaga agtcgccagg gtcatgactg aaactattga agaggtaaca ggactgaagc
     3601 cgaaaatcaa aatcaaacag gctcaaatac tcaaatcaca ttcagcaact tcaaacacat
     3661 ctacacctat caattccaat caatctacac caccccttaa taccttctat cccataattc
     3721 gtacaaaaac gctgcaatta gttgaaaatg atattgtaag atggggtcgg ttaagcggaa
     3781 aaatgaaacg gcagataaag aaatttaaaa aagataatca ggcttctgtc actgtggtgg
     3841 atgataagaa gtctgccagc tccctggctc cggctgggga gcttataaag gaatcgtgta
     3901 aaaaataaga tatggcaact gaattgacta tgactaaatc gcattcgcct catgaggtca
     3961 aattcaaatc tgacactgat cctcactttg cgatcgttga aatattgaag agggcaacag
     4021 gcgaaataaa ggcgagccac tacattttaa agagtgatgt gccgcaatgg ctatcgtatt
     4081 ttgaatcaca gggatttaca attaaaaatt cagacaatgg ataatgaact ttttgaaatc
     4141 gtttcagcgg taatcgtcag ggattcgctg accctgttca tgaataagtt aacggagcag
     4201 cacaaaggcc atcccgatta tggagcgaca atgaaacttt gcggtctgat ttataaaaag
     4261 tattcaaaga tcgcagcaac aaaatgtcag accgacgcac tgggacggat cacggtaagg
     4321 cggaacctga gatcagattt tgacctgaat taattgcaaa caacaacgaa atacaacaga
     4381 tatggcattc aaaaaaggac agagcggcaa cccagacggc aggcgaaagg gtgc
//
LOCUS       DAOOYB010000238         4414 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_23214_length_4414_cov_13.037853, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000238 DAOOYB010000000
VERSION     DAOOYB010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4414
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23214_length_4414_cov_13.037853"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(165..1367)
                     /locus_tag="PL056_11355"
     CDS             complement(165..1367)
                     /locus_tag="PL056_11355"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPM47656.1"
                     /translation="MRDELFLIHSNLLNSISMEKKRHLFNKINWDARGICIAGARGTG
                     KTTLILQHLTERYDRDKCLYVSGDNINIIAEGLFNTAKDFFAFGGETFVVDEVHKYPD
                     WSQEVKNIYDSFPQKSIIISGSSTLDLTKARYDLSRRVVYYRLEGLSFREFLKFEYDI
                     DIAPLSLEEIVKDHVSISSSILKKGPVLKYFNEYLEYGYYPYFLEGKQDYNSKVMGAI
                     EKVLFEDIGIVFNLPVNKLPAMKKLLWLVATSHPFTLNIEGIATDLGITRPTVYNYLE
                     ILENADLFKSVKALGKGAGFIRKPGKLYFENTNLLLAIAGSAKIEAQSGTLRETFFVN
                     QLSQDHKISLHEKCDFVIDEKYHFEIGGPSKKGKQISGIESSYLAIDGIETGFQKRIP
                     LYLFGFLY"
     gene            complement(1574..1987)
                     /locus_tag="PL056_11360"
     CDS             complement(1574..1987)
                     /locus_tag="PL056_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002813009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47657.1"
                     /translation="MTKNNQITGNIGLYYVCYVLSKKGFNAIPTARNAKGVDIIVYNE
                     DCSKMVGVQVKTLSKRNPVPLGSSKKQSEKVIWVIVNEVRAEKHNTYIMTSEEIEEKT
                     VAKVKNGKTSCWLQPKDYCKNEYSEKWDKIMNKLK"
     gene            complement(1984..2799)
                     /locus_tag="PL056_11365"
     CDS             complement(1984..2799)
                     /locus_tag="PL056_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47658.1"
                     /translation="MKDCDKAKAKQITDASYEYLKIIAQDIDNNEEIIQRHLALPKNC
                     KMNMKDVFKRMIISSANRQMGKNVILKRTEKDGDEIGILTDVLFDFDHNKVITKYGED
                     CETLLNELVKIVGEVRKEKNSLWPKFCKTILSSGKFLNKFNDFSDFESWVIFFNKDSR
                     SRVALPMIISKEVEGFGFALACDFLKEIGYEGFGKPDVHIKEIFYGLKLSNSKEDYFV
                     FKDLVEFATLADKSPFYIDKLFWLCSTGNFHYDKIEVKTNRNDFIKFLKKENL"
     gene            complement(2876..3820)
                     /locus_tag="PL056_11370"
     CDS             complement(2876..3820)
                     /locus_tag="PL056_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47659.1"
                     /translation="MKMEKISAENILFIKLGRGGDFEEKCIKEKTLKLGYSEVDYELC
                     KKGDWKKVQKIFLEKKCSLSVATNHMNQIKQFYTADEKTLWITFHANNLWWCFSKPEI
                     TICSDGTKTRPVTDKWFNTDTDGKTLSFDRLSGKLLKTQGYRGTICKVEAKDYLINKI
                     NGTPSKKLEGAMGSISDLEKTLKDLIGELSWQDFEILVDLIFRNGGWQRSSTLGDQLK
                     TIDLDLMSPLTGDKIAVQVKSKSDKKEFEQYLENFKNFKDYRRFYYFVHQPENDLRNY
                     SEITKIKDGVKVHLVFGEKISKMIISSGLIDWIINKSA"
     gene            complement(3834..4226)
                     /locus_tag="PL056_11375"
     CDS             complement(3834..4226)
                     /locus_tag="PL056_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47660.1"
                     /translation="MERISIFEKNLRKFAILDNAEGDINTIWQDERTGEFFIESMLEK
                     CSGYVYFKASKENILKFIEQGITLEELYAAESNDEIFVNVFKNHNIEKPRNTKNLEIN
                     FHCGGKYRMIGMYDEKIEEIKRKLMEFR"
BASE COUNT         1272 a          838 c          661 g         1643 t
ORIGIN      
        1 ccggacaaaa tctcttatat ctttcactat actgaaatct ttgtcaagtt ttatgtcgca
       61 tctttcagta taatgaaatc tgtcgagtgt tttggtgcta acctttcagt ataatgaaat
      121 ccttgaagag aaaagtgtca aacgggtttt acagaaatgg ggtttcagta aaggaatccg
      181 aaaaggtata gcggtatccg cttttgaaaa ccggtttcaa ttccgtcaat ggcgagatag
      241 cttgattcaa ttcctgaaat ttgttttccc ttttttgaag gacctccaat ttcaaaatga
      301 tatttttcgt ctataacaaa atcacatttt tcatgaagtg atattttgtg gtcttgtgaa
      361 agctggttca caaaaaaagt ttctctcaat gttcctgact gagcttcgat ttttgctgat
      421 cctgcaattg caagaagaag atttgtattc tcaaaataaa gtttccctgg ttttcttata
      481 aatccggcac cttttcccaa tgctttaaca gatttgaaca gatcggcatt ttccagtatt
      541 tcaagatagt tataaacagt cggtcttgta attccaagat cagttgctat tccttcaatg
      601 ttcagtgtaa aagggtggga agttgcaaca agccataaaa gttttttcat tgccggcagt
      661 ttattaacag ggagattaaa tacaattccg atatcttcaa aaaggacttt ttctattgct
      721 cccataacct tgctgttgta atcctgcttt ccttcaagaa aataagggta gtatccgtat
      781 tcaagatatt cgttgaaata ctttaatacc ggtccttttt ttaagattga agaggaaatt
      841 gaaacatggt ctttcactat ttcttcaaga gataaaggag caatatcaat atcatattca
      901 aatttcaaaa attctctgaa agatagtccc tcaagccggt aatagacaac tcttcttgaa
      961 agatcatatc tcgcttttgt aagatcaagt gtggaacttc ctgaaataat tattgatttt
     1021 tgaggaaaag agtcatagat gtttttgact tcctgtgacc agtccggata tttatgaacc
     1081 tcatcaacaa caaatgtttc gccaccgaat gcgaaaaaat cttttgcagt attaaaaaga
     1141 ccttccgcaa ttatgttgat gttatctcct gaaacataaa ggcatttatc ccgatcatat
     1201 ctttctgtca gatgttgaag aataagggtt gtcttgccag ttccccttgc acctgcgata
     1261 cagattcctc ttgcgtccca gtttatctta ttgaaaagat gtcttttctt ttccatgctg
     1321 atactgttca atagatttga atgaatcaga aaaagttcgt ctctcatcac cgcctccctg
     1381 caaaagtgtc aaacgcattt tacactaatt ggctgaaaat gtcaaacggg ttttacatgt
     1441 ttgggttgaa agtgtcaaat gcgttttacg gaatccactc tgccggacca agtttgtaga
     1501 aatatttaag cattttataa accttctcct taaaatcacc ataacctata tgaaaaacgg
     1561 gaggattcaa cagttatttc aatttgttca ttattttatc ccatttttca ctatattcat
     1621 ttttacaata atcttttggt tgaagccagc atgatgtttt cccatttttt acttttgcta
     1681 cggtcttttc ttcaatttct tcggaagtca taatgtaagt gttgtgtttt tcggcacgca
     1741 cttcattgac tatcacccat ataacttttt cagactgttt tttgcttgaa cccaaaggaa
     1801 caggatttct tttgcttaag gtctttactt gaactccaac catttttgaa cagtcttcat
     1861 tgtaaactat tatatcaacg cctttagcat ttctagccgt tggaatagcg ttaaaccctt
     1921 tttttgaaag aacatagcaa acatagtaaa gaccgatgtt accggtaatc tgattgtttt
     1981 ttgtcataga ttttcctttt ttaaaaattt tataaaatcg tttctgtttg ttttcacttc
     2041 tatcttatca tagtgaaaat ttccagtcga acaaagccag aagagcttgt caatgtaaaa
     2101 gggggatttg tctgccaagg ttgcaaactc tacaagatct ttaaaaacga aataatcttc
     2161 tttggaattt gaaagcttca acccgtaaaa tatctcttta atgtggacat cgggctttcc
     2221 aaaaccttca tatccaatct cttttaagaa atcacaagca agagcaaatc caaatccttc
     2281 gacttctttt gaaattatca ttggcagtgc gactctgctg cgagaatctt tgttgaaaaa
     2341 aattacccag ctttcaaaat cactgaaatc attaaattta tttagaaatt ttccagagct
     2401 taatatagtt ttgcaaaatt ttggccacaa actgtttttc tcttttctga cttctccaac
     2461 aattttaacc agttcattta aaagggtttc acaatcttcc ccgtactttg taattacttt
     2521 gttatgatca aaatcaaaaa gaacatccgt taaaattccg atttcatcac catctttttc
     2581 ggttcttttc agaattacat tttttcccat ctgtctattt gcagaagata tgatcattct
     2641 tttaaaaaca tctttcatat tcattttgca atttttaggc aatgcaagat gtctctgaat
     2701 tatttcttca ttattatcaa tgtcttgcgc tattattttt aaatattcgt aagaggcatc
     2761 tgttatttgt tttgcttttg ctttatcaca gtctttcacg atctttctcc tccaaattta
     2821 tttgcttgtg ctcaaataca tcaacaatcc cgtttttttc atgtgaatga taagttcatg
     2881 cagacttatt tattatccag tctataagtc cagatgaaat tatcattttt gaaattttct
     2941 ccccgaaaac aaggtgaact ttaacaccat cttttatttt tgttatttct gaataattgc
     3001 gtaaatcatt ttctggctga tgaacaaaat agtagaaacg gcgataatct ttgaaatttt
     3061 taaaattttc aagatattgc tcgaattcct ttttatctga tttcgattta acctggacag
     3121 cgattttatc tcctgttaat ggtgacataa gatcaagatc aattgttttt aactgatctc
     3181 cgagcgtgct gcttctttgc catccgccat ttctaaagat gagatcaaca agaatctcaa
     3241 aatcctgcca tgacaattca ccaattaaat ctttcaatgt tttttcaaga tctgaaattg
     3301 aacccatagc tccttcaagt tttttactcg gagttccatt aatcttgtta attaaatagt
     3361 cttttgcttc aactttacag attgtgcctc tatatccctg tgttttaagc aattttccgc
     3421 tcaaccggtc aaaagacaag gtctttccat cagtatctgt attaaaccac ttatctgtaa
     3481 ctgggcgggt tttggtgcca tctgaacata tcgtgatttc tggttttgag aaacaccacc
     3541 acagattatt tgcatgaaaa gtaatccaaa gggttttttc atccgcagta taaaactgtt
     3601 ttatttgatt catatggttt gtagcaacag acaaactgca ttttttttct aaaaagattt
     3661 tttgtacttt tttccagtct ccttttttgc aaagttcgta atcaacttcg gaatatccga
     3721 gctttaaagt tttttctttt atgcattttt cttcaaaatc accgcctcga cccagtttaa
     3781 tgaacaaaat attttctgca ctgatctttt ccattttcat tgtctttttc tcctcaccta
     3841 aactccatca acttcctttt gatttcttca atcttttcgt catacattcc gatcattcta
     3901 tattttccgc cacaatgaaa gtttatttct aagttttttg tatttcgagg tttttcaata
     3961 ttgtgatttt tgaaaacatt tacaaaaatc tcatcgtttg attctgccgc ataaagttct
     4021 tcaagtgtga ttccctgctc aatgaatttc aggatgtttt cttttgatgc tttgaaataa
     4081 acatatccgc tgcatttttc gagcattgat tctataaaga attcaccggt tctttcatcc
     4141 tgccagatag tgttgatgtc gccttccgca ttgtcaagaa tagcgaattt ccttaaattc
     4201 ttctcaaaaa tactaattct ttccatgatt ccctccacaa attgatgaac aataacctct
     4261 tttctttcga ggtctaattt tattttattc aaccaatgcg acacaggttg tcgtgattaa
     4321 gatagattaa gattcctgat taactacact tctgaatttt tgaagacgaa aggatagttg
     4381 actgtgacta ttccgcctcc tttgggtttg ggga
//
LOCUS       DAOOYB010000239         4386 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_23421_length_4386_cov_5.231586, whole genome shotgun sequence.
ACCESSION   DAOOYB010000239 DAOOYB010000000
VERSION     DAOOYB010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4386
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23421_length_4386_cov_5.231586"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(176..>4386)
                     /locus_tag="PL056_11380"
     CDS             complement(176..>4386)
                     /locus_tag="PL056_11380"
                     /inference="COORDINATES: protein motif:HMM:NF024807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HPM47661.1"
                     /translation="KLADSSANTSTGSHGTVYVDRTAPAITIDLNKNAFNSSDIIRVS
                     VTSSEELVSAPAVEIPEISMSLSNVTQVSNFSWIFTVDPADLIDDDYTVTAEGVDLVS
                     LTGSDIAYFSVDSSIPDLVTGTIDRTKAGLNITYTVDFEITEPVAGGIPVVKANDIVL
                     TNCVQNGSDKIFKCSRTVGAEETDSTVYAVIATLTDEAGNSRTVSIGTLLIDKIPPNV
                     VPGSAYLSIDAGANCELAQNQIKSLGNGSSAHISLQLTEKLETGFTPVLVARKSGAAD
                     RVFTLDAQSGLTLYFSHSYNETNPPEEYSGEYALYLSLKDEAGNVAEPIINSDKSFKI
                     AQIKEPKLVINQDLVSYTRSPWGRSKEYELKDEGGITQYTVPANEYYELGPVNFYEAT
                     DRLPAETFILSFGGTTTESPVKIKVKDKDLVDINSRFNSPNHDGTWPRTRIWSYDSKE
                     IYVSGIDGSCRETPPKKVRNVNWITSMNKKIIGNTEINPHKFETTDLFFKKDGLAQRN
                     LQEPEIYDENEGQVKIITNVVMSFHDWKKQPVINRYSHDMIYDSFKNNIIVFGGYNGL
                     ECNNETWKWNGVFWTQLNPVDKPSKRYGHSMSFDSNRGKLVLFGGYNGDSSNNETWEW
                     DGYNWNLMNPVTKPVARYNHAMIYDSDRKKTILFGGSSGGDETWEWDGTNWFKANPTV
                     IPSVRSGHKMVYDQIKKRVYLFGGISSIWPYFPTSETWEWNGLNWTKKNPVTKPAARY
                     NHAMVYDSAKAKTILFGGRDFSGGIIGENHDYYDDSWEWDGTNWTKLNPVNNPSARSG
                     HAIAFDSFKKKIILLGGYKKDNDVLSQDYIYSDTWEWDGANWIPIISSNIKSPVYPPD
                     IKEHSMIYDIKNGKTMIWGGISYTAESYFHDEYDEIFSDTFEWNGTFWDKKTTLNSPS
                     ERYGHATAYDILNQTTVLFGGYDYDGFEKGDTWLWNDTNWELASSNTGRSRHAMTYDR
                     ARSKVVLFGGYKTSYKDDTWEWNGSYWLSKNPSTKPSARREHSMTYDVKREKVVLFGG
                     FTGANNNETWEWDGNNWTQKNPLNKPSARYNHSMVYHNYYGKVILFGGYNGSVTNNET
                     WEWDGENWNQLNLIIKPSARQGHSMAFDSIEGKVLMFGGSSGDTGMWAFESGWNDRPG
                     QIFTSSVYHYQQPDIFQYSKVYFRSIFTSFNAGGVGDVQGSAINGVDLIAWKNGSWET
                     VSSNDAGVDDLKSVLGIFSNDHEEIKSILNGNSSNIYIAAIPKAPRGFGTVEGQIASD
                     YMEMILSYTILEPDDFAPTGHLDGAGADFMWGWACDKDTPEENIHVVLSFYTGETYLT
                     SIDIGETNNPSEQGVQDICGEGSTTHRFSFNPSTSAELQQALHDKFISRPVTLTVHAY
                     GMNTGPVPPGSNTELGDSPKEFVY"
BASE COUNT         1252 a          949 c          718 g         1467 t
ORIGIN      
        1 aatcaataaa ctgcgcaata ttaatcaatt tttacagata tgccagaatt gttttacaga
       61 tcaaattccg ccatttcctt tttactgtcc gacacatttt tttgccattt aacaggttca
      121 gatatagaaa aatgatgtcc tggtaataaa atgactgtga aaccaacaaa tttatttaat
      181 acacaaactc ttttggtgaa tctcctaact cagtgtttga ccctgggggc actggtccgg
      241 tgttcatgcc gtatgcgtga actgttagtg tcactggtct tgagatgaat ttgtcatgca
      301 atgcctgctg aagttctgca cttgttgagg gattaaatga aaaccgatgg gtagttgaac
      361 cttcaccgca aatgtcctga actccctgct ctgacgggtt attcgtttca ccgatgtcaa
      421 tggaagtcag atatgtttcg ccagtgtaaa atgataaaac aacatgaata ttttcttccg
      481 gagtgtcttt gtcacaagcc caaccccaca taaaatcagc accagcaccg tcaagatgac
      541 ctgtcggagc aaaatcatca ggttcaagaa tagtataact caaaatcatt tccatatagt
      601 cagaggcaat ttgtccttca actgtgccaa aaccacgggg agcttttggg attgcggcaa
      661 tataaatgtt tgaactattg ccattaagaa ttgattttat ctcttcgtga tcgtttgaga
      721 aaattcctaa aacggatttc agatcatcaa ctcccgcatc atttgaagaa accgtttccc
      781 atgaaccgtt tttccatgcg atcaggtcaa caccgtttat tgctgaaccc tgaacatcac
      841 ctacaccacc agcgttaaaa gaggtaaaaa tcgaacgaaa atatactttt gaatattgga
      901 aaatatcagg ttgttgataa tgataaacag aacttgtgaa aatttgtcca gggcgatcat
      961 tccagccact ttcgaacgcc cacattccag tatctccaga ggatccccca aacatcaaca
     1021 ccttcccttc gatactgtca aaagccattg agtgtccttg tcttgctgag ggtttaatta
     1081 tgagatttaa ttgattccag ttttcaccgt cccattccca tgtttcatta ttggttactg
     1141 atccgttata acctccgaat aatattactt tcccataata attatgatac accattgaat
     1201 ggttatatct ggcagaaggt ttatttaatg gatttttttg agtccaatta tttccatccc
     1261 actcccatgt ttcattgttg tttgcacccg taaatcctcc aaataaaaca accttttctc
     1321 tcttaacatc atatgtcatt gaatgttctc ttctggcaga gggctttgta gaaggatttt
     1381 ttgataacca atagctgcca ttccattccc atgtatcatc tttatacgat gttttatatc
     1441 ctccaaacaa aacaactttt gatcgagctc tatcataagt catggcatgt cttgatctgc
     1501 cggtattcga agaagctaat tcccaatttg tatcattcca caaccaagta tcaccttttt
     1561 caaaaccgtc gtaatcatac cctccaaaca aaaccgttgt ttgatttaaa atatcgtatg
     1621 ccgttgcatg tccatatcgt tctgatggag aattaagagt tgttttttta tcccaaaatg
     1681 tgccattcca ttcaaatgtg tcagaaaata tttcatcata ttcatcatga aaataacttt
     1741 ctgctgtata tgaaattcct ccccaaatca ttgtttttcc atttttaata tcgtaaatca
     1801 ttgaatgctc ttttatatct ggaggatata cagggctttt aatattcgat gaaatgattg
     1861 gaatccaatt tgccccatcc cattcccaag tatcactata aatataatcc tgcgaaagaa
     1921 catcattgtc ttttttgtaa cctcctagta atattatttt cttcttaaaa ctgtcaaacg
     1981 caattgcgtg accacttctg gcagatggat tgttcaccgg atttaatttc gtccaatttg
     2041 ttccatccca ttcccagcta tcatcataat agtcgtggtt ttcaccaatt ataccaccag
     2101 aaaaatctct tccaccaaaa agaatggttt tcgctttggc actatcgtaa accattgcat
     2161 gattgtatct tgcggctggc ttcgtcacag gattcttttt tgtccaattc aaaccgttcc
     2221 actcccatgt ttcagaagta ggaaaatatg gccaaattga agatattcct ccaaacaaat
     2281 aaactctttt ttttatttga tcataaacca ttttgtgacc tgatcttacc gatggaataa
     2341 cagttggatt tgctttgaac caatttgtgc catcccattc ccatgtttca tctccaccag
     2401 atgagcctcc aaataaaata gttttttttc gatcactgtc atatatcatt gcatgattgt
     2461 atcgtgcgac tggcttagtt acaggattca tcagattcca attgtaacca tcccactccc
     2521 atgtttcatt attactcgaa tctccattat aacccccaaa taacactaat ttccccctat
     2581 tactatcaaa tgacattgaa tgtccgtatc tttttgatgg tttatcaaca ggatttaatt
     2641 gagtccagaa aacaccgttc catttccaag tctcattgtt gcattctaat ccgttatatc
     2701 caccaaacac tattatatta tttttaaaag aatcgtatat catgtcatgc gaatatcgat
     2761 ttattacagg ttgttttttc caatcatgaa aactcatcac aacatttgtg ataatcttca
     2821 cttgcccctc attttcatca tatatttccg gttcctgcag atttcgctga gccagaccat
     2881 cttttttgaa aaacaaatct gtggtttcaa atttatgcgg atttatttca gtatttccaa
     2941 taattttttt gttcatactt gttatccagt taacattcct cactttcttt gggggtgttt
     3001 ctctgcatga accatctatt ccagaaacat atatctcttt gctgtcataa ctccaaattc
     3061 ttgttctggg ccatgtccca tcatgattag gtgaattaaa cctgctgttt atgtcaacga
     3121 gatctttgtc tttcactttt atctttaccg gtgattccgt tgttgttccg ccgaaagaaa
     3181 gaataaatgt ttctgccggt aaacggtcgg tagcttcata aaaattaacc ggtccaagtt
     3241 cataatattc atttgcagga acagtatatt gtgttattcc gccttcatct ttaagttcat
     3301 attctttcga ccttccccac ggtgagcggg tgtaactgac aagatcctga ttgattacaa
     3361 gtttcggttc ttttatctgt gcaattttga aagatttgtc agaattaata atcggctcag
     3421 caacattacc tgcttcatct tttagagata gataaagtgc atattctcct gaatattctt
     3481 ccggcggatt ggtttcatta taactgtggc tgaaataaag cgtcaaaccg ctctgtgcat
     3541 caagagtgaa aactctatcg gctgcaccac tctttctggc aacaagaaca ggagtaaatc
     3601 ccgtctccag tttttcggtg agctgtaaag atatatgagc ggaagaaccg tttccaagag
     3661 attttatctg gttttgtgcc aactcacaat ttgcacctgc atctattgaa agatatgccg
     3721 agccgggaac aacatttggc ggaattttgt ctatcagcaa agtacctata ctaaccgttc
     3781 tgctgtttcc agcttcatcg gtgagtgtcg ctatgactgc ataaactgtt gaatcggttt
     3841 cctctgctcc gacagttctt gaacatttga aaatcttatc tgaaccgttc tgaacacaat
     3901 tggtgagcac tatatcattt gccttgacaa ccggaattcc gcctgctacc ggctcagtta
     3961 tctcaaaatc taccgtatat gttatgttca aaccagcttt agttctgtca atggtgccag
     4021 ttacaagatc ggggattgat gagtcaactg aaaaatatgc aatatcgctg cctgttaaag
     4081 aaacaagatc aacaccttcg gctgttacag tataatcatc atcaattaaa tcagccggat
     4141 caaccgtgaa tatccatgaa aaattagaca cttgtgttac atttgacaaa ctcattgaaa
     4201 tttctggtat ttcaacggcc ggagcagaca caagttcttc ggaagaagtg actgaaaccc
     4261 ttattatgtc tgaagaatta aaagcatttt tgtttaaatc tattgttatt gccggcgccg
     4321 tcctatccac ataaactgtt ccgtgtgagc cggtcgaagt gttggcactc gaatcggcaa
     4381 gtttta
//
LOCUS       DAOOYB010000240         4385 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_23428_length_4385_cov_7.676905, whole genome shotgun sequence.
ACCESSION   DAOOYB010000240 DAOOYB010000000
VERSION     DAOOYB010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4385
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23428_length_4385_cov_7.676905"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..1074)
                     /locus_tag="PL056_11385"
     CDS             complement(64..1074)
                     /locus_tag="PL056_11385"
                     /inference="COORDINATES: protein motif:HMM:NF013269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HPM47662.1"
                     /translation="MIEGSLRRDPLHRLWVVFAPEKVDTIIQQSSENEAKFDIDPYVP
                     GLEYATGKDILSRVNPAYADKGWSSRVFPAKNPILKVENEKRSKGVGLYDVMSRIGAH
                     EVVVETPKNNIFFDQLSIEEIADAFTIMKDRVNDLKKDSRFRYIMVTKNRGLAAGGTI
                     DHNYSEIKAFPFIPPIVQYKLESCKHYYMIKERCLLCDIIEQELFDRKRVVYENDQFI
                     AICPYASRYPFEIMILPRRHAGTFEKISEESVKELAKIAKVVLCKLRKGLNSPAYNCV
                     FHFQPVNSGADEVIENLSYHWHLTIIPRLTRIPSEMLEAGVYVNPTFPEEAAEFLKNI
                     RE"
     gene            complement(1093..3270)
                     /locus_tag="PL056_11390"
     CDS             complement(1093..3270)
                     /locus_tag="PL056_11390"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HPM47663.1"
                     /translation="MTKLVFLWHMHQPYYKDTTRDLYLMPWVRLHCLKGYYDIPVSIE
                     KYGVKGVINMVPSLIEQIVDYADNGATDAWLDHTIPNPYDMKEEEKAFVIRNFFMINW
                     ERLVKPSPRYHEILNKRIRYEKKLNWSEMAKLFTNDEIFDLQMLFNLKWFGFMAREKY
                     PRLKELDEKDRGFSQKDKEDLLDIQKKIIGEIIPLYRRLAESGVIELSFTPFYHPIFP
                     LIQDSNIAARSSGAALPERFSYSEDAKWHIEEGKRYSSSIWGKEITGMWPAEGSVSPE
                     IIEPALNAGVKWIATDEGILLNSLGTQNKASVLYRPYRLKLNEEKHLTGFFRDKYLSD
                     LLGFSFSRMEPDRAVDIFMNYVRSVSFNLPQGHPEPVVSVILDGENAWESYPGNGEHF
                     IKELFTRLSRNTEIKTTTFGNITDTEDHNVMQPISRLFSGSWINSNYDIWIGNDEDNE
                     AWVYLKRVRDFYEKYRRSNIVPPKVDAEVMRCLYRAEGSDWFWWYGPQFSTENDWLFD
                     RLFRRHLRKVYNLLGVDYPTFLDIPITSSTGDTSIIGPTNVIRPAITGKNDGYYEWAG
                     AGVFENSRKTGGAMYNSIKLTDRILYGFDRDNLFFKIQLVDPLSFYEHKKYFLKGFIM
                     NGRDIDFVLPVMRINPADFAVRFNSESGYLSEVIGAGTAAFEDVLELRLNSKKSGLKP
                     GDNVKIYFKIVSSDGIELERVPVSGAIELIIPDDATLFTLWDV"
     gene            complement(3274..>4385)
                     /locus_tag="PL056_11395"
     CDS             complement(3274..>4385)
                     /locus_tag="PL056_11395"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: protein motif:HMM:NF002023.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HPM47664.1"
                     /translation="SGFMQIKVLTQFMSNSLNQHISRNWTLSPTFNQYVDSVPAQMRT
                     GDNWYLGSADAIFQNLNLIKDESPRHVFVFSGDHIYKMDVSQMYNYHNIKNADMTIAA
                     IPVPIEEATEFGVIEIDKDYRMIGFEEKPKNPKSIPGRPDTALVSMGNYLFSTDILVN
                     AVTEDARDESSVHDFGKNVIPALFPYKRVFVYDFLKNSIYMPTENDYEKPYWRDVGTI
                     KAYFDSHMDLVSVSPAINLYNRNWPIFGFPEVNMPPAKFVFADEKNKRLGVATDSIIS
                     EGCIVSGGSVNRSVLSPGVRVNSYASLKNCIILNDVNIGRYCELKNVIVDKHVTIPSD
                     TKIGFDLKKDAQRGFTVTEEGIVVIPKGYNFQEDR"
BASE COUNT         1228 a         1000 c          845 g         1312 t
ORIGIN      
        1 atgatgaact ggggcggtaa cggccgctgg aaatgggaca gggaaaaata gtagcgggat
       61 gagttactcc ctgatattct ttaaaaattc tgccgcttct tcaggaaaag tcggattgac
      121 atagacacct gcttcaagca tttctgaagg aattctcgta agtcttggaa ttattgtcag
      181 atgccagtga tatgataggt tttcaataac ttcatccgct ccgctgttaa ccggctgaaa
      241 atgaaaaaca cagttataag caggagaatt cagccccttt ctcagtttac aaagaacaac
      301 ctttgcgatc tttgcaagct ctttgacaga ttcttcagaa attttttcaa aagtaccggc
      361 atgtcttctg ggaagtatca ttatctcaaa aggatacctg ctggcataag gacatatagc
      421 aatgaactgg tcattttcat atacaaccct tttacggtcg aaaagctcct gttcgattat
      481 atcacaaaga agacatctct ctttaatcat gtaatagtgc ttacaacttt caagcttata
      541 ttgaactatc ggtggaataa acggaaacgc ctttatttct gaatagttat ggtctatcgt
      601 tccacctgcg gcgagacctc tgttctttgt caccattatg tatctgaaac gggaatcctt
      661 tttaagatca ttcaccctgt ctttcattat ggtaaaagca tcggctatct cttcgatcga
      721 gagctggtca aagaatatgt tgttcttagg agtttcaaca acaacttcgt gtgctccgat
      781 ccttgacatc acatcataca gccctacacc tttgcttctc ttttcgttct caactttaag
      841 gatcggattc ttcgccggga aaactcgtga agaccaccct ttgtcggcat atgcaggatt
      901 aactctgctt aagatatcct ttccggtcgc atattcaaga ccgggaacat aaggatcaat
      961 atcaaatttg gcttcatttt cagagctttg ctgtatgatc gtatcaactt tttccggagc
     1021 gaaaaccacc cacaatctgt gaaggggatc gcgtcttaaa cttccttcta tcattatttc
     1081 accttcaaaa aattaaacat cccaaagtgt aaaaagggtc gcatcatcag gaattataag
     1141 ttctatcgca cctgacaccg gaaccctttc aagctcgatc ccgtcacttg aaactatctt
     1201 gaaatatatt ttcacattgt cacccggctt cagtcctgac ttctttgaat tgaggcgcag
     1261 ttcaagcaca tcttcaaaag cggcggtacc tgctccgata acctctgaaa gataacccga
     1321 ttcactgttg aagcgcactg caaagtcggc aggatttatc ctcatcacag gaagtacaaa
     1381 atcaatgtca cgcccgttca ttataaatcc tttgagaaaa tattttttgt gctcataaaa
     1441 gctaagcgga tccacaagct gaattttgaa gaacaggttg tctctgtcaa atccgtaaag
     1501 aatacggtca gttaatttga ttgaattata catggcaccg ccagtcttac gcgaattttc
     1561 aaagactccc gcacctgccc attcgtagta accgtcattt ttaccggtta tcgcaggtct
     1621 tatcacattt gtcggaccga ttatggaagt gtcacctgtt gaagaagtaa tcggaatatc
     1681 aaggaaagtc ggatagtcaa cgccgagaag attataaacc tttctcagat gtcttctgaa
     1741 aagccggtca aaaagccagt cattttcagt agagaactgc ggaccgtacc accagaacca
     1801 gtcactccct tcagcacggt agagacagcg cataacctcc gcatcaactt tcggaggaac
     1861 aatattgctc cttctgtact tctcataaaa atctcttacc cttttaagat aaacccatgc
     1921 ttcgttatct tcgtcattgc cgatccagat gtcgtaattg ctgtttatcc acgaaccgga
     1981 gaacaaccgg ctgatcggct gcatgacatt atgatcttca gtatcagtaa tattcccgaa
     2041 agtggttgtc tttatttcag tatttctgct cagtcttgtg aatagctctt ttatgaaatg
     2101 ctctccgttt ccaggatatg attcccatgc gttctcaccg tcaagaataa ctgaaacaac
     2161 gggttccggg tgtccctgcg gcagattgaa gctgacactt ctcacatagt tcatgaaaat
     2221 atcgacagct ctgtcgggct ccattcttga aaaagaaaaa ccgagaagat cggaaagata
     2281 tttgtcacgg aaaaaaccgg ttaaatgctt ttcttcattc aatttaagcc ggtaaggtct
     2341 gtaaaggacc gacgccttat tctgcgttcc cagagaatta agcagtatcc cttcatctgt
     2401 cgcgatccat ttcacccctg cattaagagc cggctcaatt atttcagggc ttacagaacc
     2461 ttccgcaggc cacatccctg ttatttcctt tccccatata cttgaagaat atctcttccc
     2521 ctcttcaatg tgccattttg catcttcaga atatgagaat ctttcaggaa gagcagctcc
     2581 tgaactcctt gcagcaatgt tggaatcctg aataagagga aagatcggat gatagaaagg
     2641 ggtgaaactc agttcaataa ctcccgactc cgcaagtctt ctgtaaaggg ggattatttc
     2701 gccaataatt tttttctgaa tatcaagaag atcttcttta tccttttggg aaaaccctct
     2761 gtccttttcg tcaagctctt tcagccgggg atatttttcc cgtgccatga acccgaacca
     2821 tttgagattg aaaagcatct gaagatcaaa tatttcatca ttggtaaaaa gcttcgccat
     2881 ttcgctccag ttgagctttt tctcgtaacg gatcctttta ttgagtatct cgtggtatct
     2941 cggagacggc ttgacaagcc gttcccagtt gatcataaaa aagtttctga tcacaaacgc
     3001 tttttcctct tccttcatat cgtaaggatt ggggatcgta tgatcaagcc acgcatcagt
     3061 tgctccgtta tcagcataat caacgatctg ctcaataagt gacggaacca tgttgatgac
     3121 acctttgaca ccgtatttct cgatcgaaac aggaatatca taataaccct tcagacagtg
     3181 aagacgaacc cacggcatga gataaaggtc ccttgtagtg tccttgtaat acggctgatg
     3241 catatgccac agaaaaacca gttttgtcat aaattatctg tcctcctgaa aattgtatcc
     3301 tttcggaatt acaactattc cttcttctgt aacagtaaaa cccctttgag catctttttt
     3361 aagatcaaaa ccgatctttg tatcagacgg tatggtcaca tgcttatcaa caatgacatt
     3421 tttaagttcg cagtatcttc cgatattgac atcattgaga attatgcagt tttttaaaga
     3481 tgcatagctg ttcactctca cccccggtga aagaactgaa cggttgacac tgccgcctga
     3541 aactatgcag ccctctgaaa ttattgaatc tgtcgcaaca ccgagccgtt tgttcttttc
     3601 atcggcaaag acaaattttg caggaggcat gttaacctca ggaaaaccga atatgggcca
     3661 gttcctgtta tagaggttta tcgcgggcga tactgagaca aggtccatat gcgaatcaaa
     3721 atacgctttt attgttccga catctctcca gtaaggtttt tcataatcat tttcagtcgg
     3781 catatagatg ctgttcttga gaaaatcata aacaaaaacc cttttgtacg ggaaaagtgc
     3841 cggaatgaca ttctttccga aatcgtgaac gctcgattca tctcttgcat cctctgtcac
     3901 agcatttaca aggatatctg tcgaaaagag atagttgccc atgctgacaa gagcagtgtc
     3961 cggtcttccc ggaattgatt tcgggttttt cggcttttct tcaaaaccta tcatcctgta
     4021 atccttgtct atctcgatca caccgaattc agttgcctct tcgatgggaa caggaattgc
     4081 cgcaattgtc atatcggcat tcttgatatt gtggtaatta tacatctgcg aaacatccat
     4141 tttgtagatg tggtctccgc tgaaaacgaa cacatgacgc ggtgattcat cctttatcag
     4201 atttaggttc tgaaatatcg catcggcact tccaagatac cagttgtcgc cggttctcat
     4261 ctgtgcaggg actgaatcga catactgatt aaaagtgggc gaaagagtcc agttccttga
     4321 aatgtgctgg ttgagtgaat tgctcatgaa ctgggtcaga acttttatct gcatgaaacc
     4381 ggagt
//
LOCUS       DAOOYB010000241         4358 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_23632_length_4358_cov_10.952359, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000241 DAOOYB010000000
VERSION     DAOOYB010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4358
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23632_length_4358_cov_10.952359"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1256
                     /locus_tag="PL056_11400"
     CDS             <1..1256
                     /locus_tag="PL056_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47665.1"
                     /translation="WKDLNKEVYDKYKNGEFEDNEYIAECYPWPCEWKVEPTPNERVP
                     LTAHQKCDKFLNPRTWANDFGGQNRASNMDDGKIAMQLYNYRYSDVSYESQSGRYTGK
                     RTVLYNITSGKYTVIGKSVFPFYINGHIVIDPYITVNSKHFSVLVNAVPYKESYKYEV
                     IYTDEDTSVLLDSSPYITDKWTIMVVNHLDDENDPMGAGTRELIYSKTGEWKWTTLAH
                     GNSDGKAGELSIAGDKAMFAHIATNTSYICDLSKSPKSLSDCKKISREGESAGFPKFD
                     RDNPNRIIYRSIVEGGPANKMVVVDISKEPWKIEKEFEIPSTETAFLSIWLMEFKSNV
                     ILYEENYVMNESGYQEDGKLCYYRIDKGKTYCSKPIENQTSYGHGYATFENKYLFWQP
                     MYKAGYILRDMECYCKEEGVCPFEE"
     gene            1374..3422
                     /locus_tag="PL056_11405"
     CDS             1374..3422
                     /locus_tag="PL056_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47666.1"
                     /translation="MKRSTTSILILMTALFFMSACSYVTRPVASTPELTWEEVEAKYH
                     SLEAVSDHAGIETFSDEMLKLRWAKWQYEFIKFRLAKSFFNRGEYDRSLEIFNELKGV
                     KPFVNETHLTAGEAALKTGQYDESLKWVLSIYLKLGKEEKVRGSKTVLLAYLYSKRVE
                     KAATWYSALDDVKKENAKAELEEWMNRSQTNRSDFNNYLSMEPGGKSGDIPENMKEEV
                     LDEMKKLEEGSEIIEEMPIDETYDAARVPDWNNLCVALSNDEKWTKFNEVITSFITWY
                     FKDYRKSGIQLTFLNYLDETDIAKLFEKAKETKCFAVAGPFFAPEFAQTFKERSLETA
                     IPVVSYIPYISTLKGIFYNVMPSKDSESENIIRYAVNEREKKKFAIAYLDNREGRILR
                     DMYWKNIEDMGGSVTDLLDLSPGDNAFFDDVEKVIGKPDNYDEAIRIFKWKNKDKFSS
                     DILMKRAIDRFTKTIPGKCDFDALVVATPVFHMPMFLPSFPYMNVEFEYYQKYLNRNV
                     AVRKHNLREEGYDWDIQQILVIAPSELVSSEKVIEQLGTTIDGMTVYAPVNNFSDSNK
                     PYSEITKSFSEKNGRGLYFIENFIAEVSEILFNGLERSGKKDISGFVSALNGTEFTSI
                     LSGTPVKFDEYNRMTGRSAVMIGRNKEPFMTPAQLEEELRKKEEEKKKEEEKKKEVKQ
                     "
     gene            3423..4139
                     /locus_tag="PL056_11410"
     CDS             3423..4139
                     /locus_tag="PL056_11410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPM47667.1"
                     /translation="MLRYSAGLIFLIFYVIWGALLYPFYILSGKKTGKYLVRNLFIVV
                     LKICGVKIVAEGLENVNENEIYLVVGNHQSVFDIPVIGAVLPLNLRIFAKKELSRIPV
                     FGQLMVLYDFVFVNRNNQREAVRDLKKAAQKMEHCSFLVFPEGTRSKDGRVGKFKSGA
                     FSLAFETGKTILPVALTGVANIMRPGKLTVNKGTVYMKIFPPVKIEEGDSRKSVAEKL
                     QLIISEHVHSKGGLNEKDQC"
     gene            4120..>4358
                     /locus_tag="PL056_11415"
     CDS             4120..>4358
                     /locus_tag="PL056_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine 6-transaminase"
                     /protein_id="HPM47668.1"
                     /translation="MKKINAKQVHEILGKYILCDGYDIVVDAEKSHGSYLYDAKHKKE
                     YLDFFSFFASMPLGFNHPKMNNRKFIEEIGRISLH"
BASE COUNT         1488 a          739 c          970 g         1161 t
ORIGIN      
        1 tatggaaaga tcttaataaa gaagtttatg ataaatataa aaacggggag tttgaggaca
       61 atgaatacat tgcagagtgt tatccctggc cgtgtgaatg gaaagttgaa cctacaccaa
      121 acgaaagggt cccgttaaca gcccatcaga aatgtgacaa gttcttaaat cccagaacat
      181 gggctaatga ttttggtgga cagaacaggg caagcaatat ggatgatggg aagattgcaa
      241 tgcagttgta caattacagg tatagcgatg taagttatga gtcacaatct ggcagatata
      301 caggtaaaag aactgtgctt tataatataa catcaggtaa atacacagta ataggaaagt
      361 ctgtttttcc tttttatatt aatggtcata ttgtaattga cccgtatatt acagttaaca
      421 gcaaacactt cagtgttctt gtaaatgctg ttccctacaa agagtcttat aaatatgaag
      481 ttatatacac tgacgaggat acaagtgttc tgttagattc aagtccatat ataacagaca
      541 aatggacgat tatggttgtt aaccatctgg acgatgaaaa tgatcctatg ggagcaggaa
      601 cccgagaact gatttattca aaaacagggg aatggaaatg gacgactctt gcacatggta
      661 attctgacgg caaggcgggg gagctttcaa ttgccggtga taaggcaatg tttgcccata
      721 ttgcaacaaa cacctcatac atctgtgatc tttcaaaaag tccgaaaagt cttagcgact
      781 gcaagaaaat ttcaagagaa ggggaatctg cgggatttcc aaaatttgat agggacaatc
      841 ccaaccgcat aatttacaga tcaattgttg agggtggtcc tgccaataag atggttgtgg
      901 ttgatatttc caaagaacca tggaaaattg aaaaggagtt tgaaataccg tcaacagaaa
      961 ccgcattttt aagtatctgg ctaatggaat tcaaatcaaa tgttattctg tatgaagaga
     1021 attatgtgat gaacgaaagc ggatatcagg aagacggaaa gctttgttat tatagaattg
     1081 ataaaggaaa aacatattgt tcaaaaccaa ttgaaaatca gacaagttac ggacatggtt
     1141 atgccacatt tgaaaacaaa taccttttct ggcagccgat gtataaagca gggtatatcc
     1201 tcagagatat ggaatgctac tgtaaagaag aaggtgtctg cccgtttgaa gagtaaaaaa
     1261 caaatcatcc catcatataa acttaaattt aaattttagg tattaaaaga atcataactt
     1321 gattattaag attctttgta tagtatacgg ctttagaaaa agtgaggtgg cttatgaaaa
     1381 gatcgacaac ttccattctg atcctgatga cagcgctttt ttttatgtca gcgtgttcat
     1441 atgttacaag acctgtggct tcaacgccgg aactcacatg ggaagaggtt gaagcgaagt
     1501 atcactctct tgaggcagtt tcagatcacg cagggattga aacattttca gacgaaatgc
     1561 ttaagctcag atgggctaag tggcagtatg aattcataaa attcagactt gcgaaatcat
     1621 ttttcaaccg tggtgaatat gacagatctc ttgaaatatt caatgaactc aagggtgtaa
     1681 aaccttttgt gaacgaaact catcttacgg caggcgaagc ggctcttaag accggccagt
     1741 atgatgaatc tctaaaatgg gtcctttcaa tatatctcaa actcggaaaa gaggagaagg
     1801 tcagagggtc aaaaacagta ttgcttgcct acctttattc caaaagggtt gaaaaagcgg
     1861 cgacatggta ttctgctctt gacgatgtga aaaaggaaaa tgcaaaagct gaacttgaag
     1921 agtggatgaa cagatctcag acgaatagaa gtgatttcaa caactatctg tcaatggagc
     1981 caggcgggaa aagcggggat attcctgaaa acatgaaaga ggaagttctc gatgaaatga
     2041 aaaagcttga agaaggttct gaaataattg aagaaatgcc gattgatgaa acttatgatg
     2101 cagcccgggt gcccgactgg aacaatctct gtgttgcact ttcaaatgat gaaaaatgga
     2161 caaagttcaa cgaagtgatc acttcattca tcacctggta tttcaaagat tacagaaaga
     2221 gtggaataca gcttacattt ttaaattatc ttgatgaaac cgacattgcc aaactgtttg
     2281 aaaaggcgaa agagacaaaa tgcttcgccg tcgcaggacc ttttttcgcc cccgaatttg
     2341 cacagacatt caaagaaaga tcgcttgaga cagcaatacc tgttgtttcc tacatacctt
     2401 acatctcaac attgaaaggg attttctata atgtcatgcc gtcaaaagac agcgaatcgg
     2461 agaacattat cagatatgct gttaatgaaa gagaaaagaa aaaattcgct atagcatatc
     2521 ttgacaacag ggaagggcgg atactcagag atatgtactg gaagaatatt gaggatatgg
     2581 gcggaagcgt gacggatctg cttgatctct cgcccggaga caacgccttt tttgatgatg
     2641 tggaaaaggt gatcggcaaa ccggacaact atgatgaagc gatcaggatc tttaaatgga
     2701 agaacaaaga taaattcagc agtgacatac tcatgaaaag ggccattgac cgctttacca
     2761 aaaccattcc aggcaaatgc gattttgacg ctcttgttgt cgccacacct gtttttcaca
     2821 tgccgatgtt ccttccttcc tttccctaca tgaatgtcga atttgaatat taccagaaat
     2881 atctgaaccg gaatgtggct gtgagaaagc acaatctgcg cgaggaggga tatgactggg
     2941 acattcagca gatacttgtt atcgcacctt ctgagcttgt aagtagtgaa aaggtgatag
     3001 aacagcttgg aacaacaatt gacggaatga cagtctatgc ccctgtcaac aacttttcag
     3061 attctaacaa gccatattcg gagataacaa aaagtttcag tgaaaagaac ggaagaggtc
     3121 tttatttcat tgagaatttc attgccgaag tgagtgaaat actgttcaac ggacttgaaa
     3181 gatcgggtaa aaaagatatt tcagggtttg tttctgcact taacgggact gaatttacat
     3241 cgatcctttc aggtacaccg gtaaaatttg acgagtataa cagaatgacc ggaaggagtg
     3301 cagttatgat aggccgcaac aaggaacctt tcatgacacc cgctcaactg gaagaggagc
     3361 tcagaaagaa ggaagaggaa aagaaaaagg aagaggaaaa aaagaaagag gtcaaacagt
     3421 aaatgctcag atattccgca ggacttattt ttctgatctt ttatgttata tggggagctc
     3481 tgctttatcc gttttacatt ttaagcggca aaaagacagg aaaatatctt gtgagaaacc
     3541 ttttcattgt cgttttaaaa atatgcggag tcaaaattgt tgccgaaggt cttgaaaatg
     3601 ttaatgaaaa cgagatatat cttgtagtag gcaaccatca gagcgttttc gacatacctg
     3661 tgatcggagc ggtactgccc cttaatttga ggatctttgc aaaaaaagaa ctttcgagaa
     3721 tacctgtttt cggacagttg atggtccttt atgattttgt gttcgtgaac aggaataacc
     3781 agcgtgaagc tgtgagagat ctcaaaaaag cggctcagaa aatggaacat tgttcgtttc
     3841 ttgtctttcc ggaagggaca agatcaaagg atggaagggt cggaaaattt aaaagcgggg
     3901 cattttcgct tgcatttgaa acaggaaaaa caatacttcc cgttgctttg acaggtgttg
     3961 caaacattat gcgtccgggg aaactcacag ttaacaaggg aacagtctat atgaaaattt
     4021 tccctcctgt gaaaatagaa gagggggact caagaaaaag tgttgctgaa aaacttcagt
     4081 taataatatc agaacatgtt cattcaaaag gagggttaaa tgaaaaagat caatgctaaa
     4141 caggttcatg aaattctggg aaaatatatc ctttgtgacg gatatgacat tgtcgtggac
     4201 gcagaaaaaa gtcacggaag ctatctttat gacgcaaaac acaaaaagga atatctcgat
     4261 ttcttttcgt tttttgcatc aatgccgctt ggattcaatc acccgaaaat gaataacagg
     4321 aaattcattg aggagatcgg acgcatttct ttgcacaa
//
LOCUS       DAOOYB010000242         4357 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_23638_length_4357_cov_13.167364, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000242 DAOOYB010000000
VERSION     DAOOYB010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4357
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23638_length_4357_cov_13.167364"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1002
                     /locus_tag="PL056_11420"
     CDS             <1..1002
                     /locus_tag="PL056_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47669.1"
                     /translation="NLYSICYEKKKAFENYVVKSDFYLKHERMDEAEDALKKYLKCYP
                     EKEVFIKLAKLYESQKKYYLAGLTYKTAGLEKKYAEVEKRRISKLTQTTFHNFNSRTN
                     TKVKEFKKTGKELKNLSIALFSIGSVLAAAGGGIFISNKAFDGKTSELIQYSFLIGGF
                     SIIGAGFSTNFNGDYYINKSISLENFNTKEIVDHGTTMEEYFVHTGAQNYTKKMSAKS
                     LRNTGIFMLVVSVPILTFSMFSLFDDYKNSFNYGRDDYSESSLHDALTEVLIVNPVKI
                     LLMPPALLFMVRGIIMLAKSSKYKKLSAEPSLLTLNSIAPIIDPVSKTYGLALGFSF"
     gene            1127..3913
                     /locus_tag="PL056_11425"
     CDS             1127..3913
                     /locus_tag="PL056_11425"
                     /inference="COORDINATES: protein motif:HMM:NF019874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HPM47670.1"
                     /translation="MDTGISVKIELHCHSAISDGLLTPEDLASECAGSGVRFASLTDH
                     NTTAGLDKFESACRHHGIGFISGIELSSVIGNTEIHLLGYGFDRTSDAFTSILAPVGG
                     GILPAAEIIKRIHRAGGIAVLAHPFAYELDNERVEKLINDLADTGLEGVEVFHHSATD
                     EQQKFLQNIVKKQGLTVSGGSDFHSSGETLPNMPGIQFPMEAWRSFRKTLFSRKPSPE
                     PSLKHEKTESQEITGTSRKNMGRLITWMVIPAVTVMLLFLVELFVFFLPGYEKELVNR
                     KRETIRELTNTVWSMLDEANVEIKAGADPEKTKQHAAEQIRALRYGPEGKDYFWVQDL
                     TPGMIAHPYRPDLDGKYIGDFTDSRGVKIFSVFAEIVRKNKEGYADYVWQWKDNPERQ
                     EAKESYLRLFEPWGWVIGTGIYLYDVSFELSVIRNRFIYTVSIIAAVLLGLLLIMIRG
                     GLLFEKQRKVAEKRLQESNDRYRSLVHAAAEGLLFVRNGLCSYANPVMLKLIGCSEAE
                     LGLIEWNELFPGFPNEPAENAKYHECQLLRLDGTDIPCTVRITGDNRFSDSFVCIVRR
                     RENNSQPVNYSSENILKKILRLPASIASDIASEIYAAKDSQEVIDLCRKTPELAVAML
                     NGGANTLAVTKAVTAITDATTRRFIELKQNEIGKEPVPFAFIVLGSQGRSEQTLFTDQ
                     DNAIIYHDDGSGDHQVETYFNELAQSVCGNLARSGYRPCGGKIMAENRKWCQPLSVWK
                     AYFSNWINRTEPKDIMELNTFLDLRCLTGNEQMVTELRDHIFKEISTHPQFFLFIANE
                     LLNFKVPLRIFADKMLDLKSAMMPVVNYARIFALKYSIPAVSTEERLSALFDNGFLSS
                     HQYQDITTVFESLLRIRLNHQAATVENGKEPDNIIDSSLLGSMDTAVLKECFKEIEIF
                     QEHIKRAFMGGLQI"
     gene            3932..>4357
                     /gene="rpiB"
                     /locus_tag="PL056_11430"
     CDS             3932..>4357
                     /gene="rpiB"
                     /locus_tag="PL056_11430"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012002392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HPM47671.1"
                     /translation="MKISIGADHGGFELKTELVKFIKSLGHTVIDNGTNSPDSVDYPD
                     YAKVVAHDILEKRADFGVLICGSGIGISIAANRFKGIRAALVTRKEYATLSRQHNNAN
                     IIAFGGRFIKFEEAKEYLEIFLLTPFEGGRHEKRVCKIDD"
BASE COUNT         1401 a          850 c          927 g         1179 t
ORIGIN      
        1 aatctttatt caatttgtta tgagaagaaa aaagcttttg aaaattatgt agttaaatct
       61 gatttctact taaaacatga aaggatggat gaagccgaag atgctttaaa aaaatacttg
      121 aagtgttatc cggaaaaaga ggttttcatc aaacttgcta agttgtatga atcgcagaaa
      181 aaatactatc ttgcagggct tacttataaa acagccgggc ttgaaaaaaa atatgctgaa
      241 gttgagaaaa gaagaatttc aaaactaacg cagacaacat ttcacaattt caactcccgg
      301 actaatacca aggtaaaaga attcaaaaag acaggaaaag aactgaaaaa tctatcaatt
      361 gctctatttt caataggttc ggttcttgca gcggcaggag gtggaatatt catcagcaac
      421 aaagcttttg atgggaaaac ttcagaactc atacagtatt cctttttaat tggcggtttt
      481 tcaattatcg gggctggctt ttcaacaaat tttaatggcg actattatat taacaagtca
      541 atatctttag aaaatttcaa cacaaaagaa attgttgacc acggtacgac catggaagaa
      601 tattttgtcc acacaggggc tcaaaactat acaaaaaaga tgtcggccaa atccttgaga
      661 aataccggga tatttatgct cgtagtttca gttcctattc tcactttttc aatgttttca
      721 ctttttgatg attacaaaaa ctctttcaat tacggtagag acgattattc tgaaagcagt
      781 ttgcatgacg ctttaactga agttctaatt gtcaatcctg ttaaaatact tctaatgcca
      841 ccagctttat tgtttatggt acgagggata attatgctcg ccaaatcctc caaatataaa
      901 aaactcagcg ccgaaccttc tcttctaact ttaaatagca ttgctccaat aattgatccg
      961 gtttcaaaaa cttacggact tgcgctggga ttttcgtttt agtcaatcga aatttaattg
     1021 aacgattgtt caattgttca tttataaaat ggtgtcgtga atactttcgc ttgacaaaaa
     1081 ttcttaaaaa atagatactt ccgttgtttt ttaatgttgg aattaaatgg atacaggcat
     1141 ttcagtaaaa atagaacttc actgtcactc tgcaataagt gacggactgc tgacaccgga
     1201 agatctcgct tctgaatgtg caggatcggg ggtgagattc gcatcgctta ccgaccacaa
     1261 cacaaccgca ggacttgaca agttcgaatc tgcatgcaga catcacggaa taggtttcat
     1321 atcaggaatt gaactctcat ctgtcatcgg aaacacagaa atccacttgc tcggatacgg
     1381 ttttgacaga acttctgacg cttttacgag cattcttgct ccggtcggag gaggaatact
     1441 gcctgccgcg gaaataatca aaagaattca ccgtgcgggg ggaatcgcag ttctggcaca
     1501 tccgtttgcc tatgaactgg ataatgaaag agttgagaaa ctcataaatg atcttgcgga
     1561 taccggactt gagggggttg aggtttttca tcatagtgca acagatgaac aacagaaatt
     1621 ccttcaaaat attgtgaaaa aacaaggtct taccgtttcc ggaggatcag attttcactc
     1681 atcaggcgaa acactgccta atatgccggg catacaattt ccgatggaag catggcgatc
     1741 gttcagaaaa accctgttct caaggaaacc ttcaccggaa ccatcgctca agcatgaaaa
     1801 aacagaaagt caggagataa caggaacttc aagaaaaaat atgggacggc ttatcacctg
     1861 gatggtcatc cctgccgtga ccgttatgct cctttttctt gttgaacttt tcgtgttttt
     1921 tcttcccgga tatgaaaagg aacttgtaaa ccgcaaaagg gagactatca gggaacttac
     1981 aaacactgtg tggagcatgc tcgatgaagc caatgttgag atcaaggcag gggctgaccc
     2041 tgaaaagaca aaacagcatg cggcagaaca gataagggct ctccgctacg gacctgaagg
     2101 aaaagattat ttctgggtac aggacctcac tcctggaatg atagcccacc cttaccggcc
     2161 tgatctggac ggaaagtaca ttggagattt cacagattca agaggggtca aaatattttc
     2221 agtattcgct gaaattgtga gaaaaaataa agaaggatac gcagattatg tctggcaatg
     2281 gaaagataat cccgaaagac aggaggcaaa agagtcgtat ctccgtcttt ttgagccgtg
     2341 gggctgggtg atagggacag gaatctatct ctatgatgtt tcttttgaac tttcagtgat
     2401 aaggaatcgc ttcatttata ctgtttcgat aatagcggct gtccttttgg gccttcttct
     2461 gatcatgata agaggaggtc tcctttttga aaaacagcgt aaggtggctg aaaaaagact
     2521 tcaggaatca aatgaccggt acagatcgct tgtccatgcc gccgcggaag gtcttctttt
     2581 tgtaagaaac gggctctgct cttatgcaaa tcctgttatg ctcaaactga tcggctgcag
     2641 tgaagcggaa cttggcctca ttgaatggaa cgaacttttc cccggatttc caaatgagcc
     2701 ggcagagaac gctaaatatc atgaatgtca actgctgcgg cttgacggaa cggacatccc
     2761 gtgtacagtc aggataacag gagataacag gttcagtgac agtttcgtat gcattgtcag
     2821 acgaagagag aataattctc aacccgttaa ttattcttca gaaaacatat taaagaaaat
     2881 tctaagactt ccggcatcaa tagcttcaga catcgcaagt gaaatatatg cggcaaaaga
     2941 ctcgcaggaa gtaattgacc tttgcagaaa aactccagaa cttgctgttg ccatgcttaa
     3001 cggaggtgca aatactctgg cagttacaaa agctgtgaca gcgataactg atgccacaac
     3061 ccgtcggttc atcgagctaa agcagaatga gatcggaaaa gaaccggttc cttttgcatt
     3121 cattgttctc ggaagtcagg gaaggagcga acagacactt tttactgatc aggataacgc
     3181 gatcatatat catgacgacg gaagcggaga tcatcaagtg gaaacttact tcaacgaact
     3241 tgcacagtca gtctgcggaa atcttgcaag atcgggatac cgtccgtgcg gcggcaaaat
     3301 aatggccgaa aacagaaaat ggtgccagcc gctgagtgta tggaaagcat atttttccaa
     3361 ctggataaac cggactgaac ctaaagacat aatggaactc aatacatttc ttgaccttcg
     3421 atgtcttacc ggaaatgaac agatggttac tgaactcagg gatcatattt tcaaagagat
     3481 ctctacccat cctcaatttt ttcttttcat cgcgaatgaa ctgcttaatt tcaaagtgcc
     3541 tctaagaatt tttgcagaca aaatgctcga cctgaaatca gcgatgatgc ctgttgtaaa
     3601 ctatgcaaga atatttgctc ttaaatattc gattcctgca gtttcaactg aagaaagact
     3661 ttctgcgctt tttgacaacg gatttttaag cagtcatcag tatcaggata taacaacggt
     3721 ttttgaatca cttctgcgaa tccggctcaa tcatcaggcg gcaaccgttg aaaatggaaa
     3781 agagccggac aacattatag attcatcact tcttggcagc atggatacag cggtattgaa
     3841 agaatgtttc aaagagatcg aaatctttca ggaacacatc aaaagagctt tcatgggcgg
     3901 attgcagatt tagtttacag gagaaaattc aatgaaaatt tcaatcggag cagatcacgg
     3961 cggttttgag ttaaaaacag aacttgttaa atttattaaa tctttagggc acacagttat
     4021 tgacaacggg acaaattcac ctgattctgt tgactatcct gactatgcaa aagttgtggc
     4081 acatgacatc cttgaaaaac gggctgactt cggagttctc atctgcggaa gcggtatcgg
     4141 catatcaatc gcagcaaaca gatttaaagg gatcagagcg gcacttgtca ccagaaagga
     4201 gtatgcgaca ctttcaagac agcataataa cgccaacatc attgccttcg gcggaagatt
     4261 cataaaattt gaagaagcca aagaatatct tgagatcttt cttcttacac cttttgaagg
     4321 gggaagacac gaaaaaaggg tctgtaagat agacgat
//
LOCUS       DAOOYB010000243         4355 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_23666_length_4355_cov_3.721628, whole genome shotgun sequence.
ACCESSION   DAOOYB010000243 DAOOYB010000000
VERSION     DAOOYB010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4355
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23666_length_4355_cov_3.721628"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..>4355
                     /locus_tag="PL056_11435"
     CDS             97..>4355
                     /locus_tag="PL056_11435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47672.1"
                     /translation="MRKVVAVLVLLMTVLFSGCEYFDNTPGSFHIKFEWNEADKPDFS
                     SKDYYAWVLLEEWPEGDREKAVLLMQTAATPFSKTGKVSIDLSDIKYGENKVIKVEIR
                     GSEKSADRVLYFGRSELFSFSSSDRDKEVKVALQTTATPGTGDDENRFNLKIIQNGNV
                     VSKINNTEVTVRINVVQGNRVILANSLSILEKYLNDKKSVTEGFADKAVSELKVFEGN
                     YYDLENWELNFGLEIAEDAGGERIVYGKLLNDDGYMSETVQVSVVVDKTAPGIINPAV
                     TPNPAKLGDIVTAVFSFTEPVDAEKLDLNWDGLNFAESESVTENVFTYTFKVDENTEE
                     RNYTLEGRAYDEIGNGPVTFTIGALTVDRTLPSISDETVSVTDDKSAIRTGDTVTVTF
                     KVSEELEKEPEVTVDGKKFKRVGDSADPYTFSYTVSDTDIEGIKTVLVSLSDLARNQN
                     VVEMEQPVKFDLSAPEVINPTVTPAGEPGNAGIGTKIEVRFNISEPVTELKFLVNGED
                     SELFNQTVSGLTYSYSRTVENNESASQYVFSVSAVDAAGNTLEPYTLGTVDIDVGMPS
                     VLAQTLSKTNVKLLEVFTLDLEFSEELKSLTVLVGSKDISSGCVKSATDLKKYNCTNT
                     ANADSDEGDGVKQFSVQMTDMSGNSSTVQLKKTGGSPLTIEYDVTPPAVVNPVIAPSK
                     ANSDSTVDVRFSFTDDVENVVMAWDGLEFIRDNSESDKKLFIYNHKVVPADEEKSYTI
                     KILEAFDIAGNPISSEIEIGTVEIDNTPPEIFNTVIKVSDDPLRTFVLENEVISISFE
                     VKEDLSDYAVRIGLKKLSVCINEDITGGKRYTCTYSTPSSVDGEGTKDVTVEVKDPAG
                     NGTSYAVGQLTYDMSDPQLSSAIVIPERVNSSHSEVKVQFSFSEDVQINVDEVYVAAE
                     NFGPELILNCNTGSGYTKQYTCTYSFLESDTRVENYDVSVDLMDAAGNKVTGMFIGKI
                     ESDREKPYLSVQNISPVAVRQNEQFTVTFTVNEELAAKPVVKIGDKQIDESSCAGSIN
                     YTCQHKANEDGDETDGVKSVTVSLRDPAGNTNIVNLSQTVTYDATRPELLNPVIIPEG
                     TANRYNSKIQVRFSFSEKIDYPASFSFTAESSNSLPVPEFVCTPSNENHQSFSCEGSY
                     ETSDTSVDTLTFKVAAKDIAGNALTVDESPDIVGTLKVDRENPVASYTSITPENIIKS
                     TASVTVVFDVSEDTAEPPVVNLGELVKTVPDNLDGNRYTYTFDDLSMLSDGDVIVSVS
                     LTDIAGNINSSPYSGNVTADRTVPSVISKTIAPTIANMNTESVTVNFTFNEKVDSFIK
                     NDVTMSPSGILTDYVCSNSSGDEQNYSCVFSMSTVGTYEGLLTFSVEAVDKAGNAMVP
                     NPVELGTLTIDTIAPELTFDSITPLTVNEDTASVTAVFTAD"
BASE COUNT         1356 a          811 c         1012 g         1176 t
ORIGIN      
        1 ctgagctcag accatttttc gggagtttaa gtcagaatcg aacgattttg gtaaatataa
       61 tatttccact ataattttca ttaccggaga gatccaatga gaaaagttgt tgcggttttg
      121 gttttattga tgacggtctt attcagcggg tgtgaatatt ttgacaacac gccagggtcg
      181 tttcatataa agttcgaatg gaatgaagca gataagccgg atttttcttc aaaagactat
      241 tatgcgtggg tacttcttga ggagtggcct gaaggtgaca gggaaaaagc ggttcttctt
      301 atgcagactg cggcaacccc ttttagtaaa acgggtaaag tttcaataga tcttagcgat
      361 ataaagtacg gtgaaaacaa ggttataaaa gttgagataa gaggaagcga aaaatcagct
      421 gacagggttc tctatttcgg tcgaagtgaa cttttcagtt tttcaagcag tgaccgtgac
      481 aaagaggtca aggtcgcact gcagacaact gccaccccgg gaacgggaga cgacgagaac
      541 agattcaatc tgaaaataat ccagaacgga aatgttgtaa gcaagataaa taacaccgaa
      601 gtgaccgtga gaataaatgt agtgcaggga aatcgcgtta tacttgctaa ttctctttcg
      661 attcttgaaa aatatctcaa cgataaaaaa tcggtgactg agggttttgc tgataaagct
      721 gtttcggaac ttaaagtttt tgaaggtaac tattatgacc ttgaaaactg ggagctcaat
      781 ttcggtcttg agatcgctga agatgccggc ggagagagaa tagtttacgg taaacttttg
      841 aacgatgatg gatatatgtc tgaaactgtt caggtgtctg ttgttgttga taagaccgct
      901 ccgggaataa taaatcctgc ggtaacgcct aatcctgcaa agctcggtga catagttacg
      961 gcagtattca gttttacaga gccggtagat gcggaaaagc ttgatctcaa ttgggacgga
     1021 cttaattttg cggaatctga gtcagttacg gaaaatgttt tcacatacac atttaaagtg
     1081 gatgaaaaca cagaggaaag aaattatacc cttgagggca gggcttatga tgaaataggc
     1141 aatggcccgg tgacttttac gataggggcg cttacagttg acagaactct tccttcaata
     1201 agcgatgaaa ctgtttcggt gacagacgat aagagtgcca tcagaaccgg tgacacggta
     1261 acggttacat ttaaagtaag cgaagaactc gaaaaagagc cggaggtcac agttgacgga
     1321 aagaagttta agagagttgg tgattcagca gatccttata ctttcagtta cactgtatcc
     1381 gatacagata tcgaggggat taaaactgtt ttggtgtctc tgtccgatct tgcgcgcaac
     1441 cagaatgtcg ttgagatgga gcagccggtc aagttcgatc tcagcgcacc tgaggtcatc
     1501 aatccgacag taacccctgc aggagagccg ggcaatgccg gaataggaac aaagatagag
     1561 gtgagattca acataagcga gccggtgacg gaactgaagt tccttgtgaa cggtgaggat
     1621 tctgaacttt ttaatcagac agtgagcggg ttgacatatt catattccag aacggttgaa
     1681 aataatgaaa gcgcatctca atatgttttt tcagtttcag cagttgatgc ggcaggaaac
     1741 acgcttgaac cctacacact cggaactgtc gatatagatg tgggaatgcc ttctgtgctt
     1801 gcccagacac tttcaaaaac caatgttaaa ctgcttgaag ttttcacgct tgaccttgaa
     1861 ttttctgaag aattgaaatc tctcactgtg ctggtcggat cgaaggatat atcttccggc
     1921 tgtgtgaagt cggcaacgga cttaaagaaa tacaactgta ccaatactgc caatgccgac
     1981 agcgatgaag gtgacggagt aaagcagttc agtgttcaga tgaccgatat gtcaggtaac
     2041 agttcgacag ttcagctcaa gaaaaccggc ggaagtccgc ttacgataga atatgatgta
     2101 acacctcctg cggttgtgaa ccctgtcata gccccttcga aagcaaacag tgacagcact
     2161 gttgatgtcc gtttcagctt taccgatgat gtcgagaatg ttgtaatggc atgggacggt
     2221 cttgaattta taagagataa cagtgaaagt gataagaaac tgttcattta taaccacaag
     2281 gttgtacctg ctgatgaaga aaaaagttat accataaaga tactcgaagc gtttgacatt
     2341 gctggaaatc cgataagttc ggaaatagag atcggaaccg ttgaaattga taatactccg
     2401 cccgagatat tcaatactgt tatcaaggtg agtgacgatc ctttaagaac attcgtcctt
     2461 gaaaatgaag tgatctcaat aagttttgaa gttaaagagg atctgagcga ttatgcggtc
     2521 agaatagggc ttaaaaaact ttcagtgtgt ataaatgaag atatcacagg cggtaaaagg
     2581 tatacctgta catattcaac gccgtcttca gttgacggag aagggacaaa agatgtaaca
     2641 gttgaagtga aagatcctgc cggcaacggc acatcttatg cagtggggca gttgacatat
     2701 gacatgagcg atcctcagct ttcaagtgcg atagtcattc ctgaaagagt taacagttct
     2761 cacagcgagg tgaaagtcca gttcagtttt tctgaagatg ttcagatcaa tgttgatgaa
     2821 gtttatgtcg cggctgagaa tttcgggccc gaacttattt taaactgtaa caccgggagc
     2881 ggatatacaa agcagtatac ttgcacatat tcattcctcg aatctgatac ccgtgtagag
     2941 aattatgatg taagtgtcga tctcatggat gcagcaggca ataaagtgac cggaatgttc
     3001 atcggaaaaa ttgagtctga ccgtgaaaaa ccatatcttt cagtacagaa catatctcct
     3061 gttgcggtaa ggcagaacga gcagttcacg gtaacattca ccgttaatga agaacttgcg
     3121 gcaaaacctg tcgtaaagat cggtgataag cagatagatg aatcatcctg tgccgggagt
     3181 ataaattata catgtcaaca caaagccaac gaagacggtg atgaaacaga tggtgttaaa
     3241 tcagtcacag taagtttaag ggatcctgca ggaaatacaa acatcgtaaa tctttcacag
     3301 acagttacat atgatgcgac aagacccgaa cttttaaatc ctgtaataat tcctgaggga
     3361 acagctaaca gatacaattc aaaaattcag gtaaggttca gtttttctga aaagatcgat
     3421 tatccggcaa gtttttcatt tactgccgaa tcttcaaaca gtctgcctgt tcctgagttt
     3481 gtctgtactc cttcaaatga aaaccatcag tcgttcagtt gcgaaggatc ttatgaaaca
     3541 tctgatacat ctgttgatac attgactttc aaagtcgccg caaaagatat tgcaggcaac
     3601 gcgctgacag ttgatgaaag tccggatatt gtaggaactc tcaaagtgga tagagaaaat
     3661 ccggttgctt cgtatacatc aataactcct gaaaatatta taaaaagcac ggcatcagta
     3721 acagttgtgt ttgatgtgag cgaagatact gccgagccgc cggttgtgaa tctcggtgaa
     3781 cttgtaaaaa cagttcctga taatctggat ggaaatcgct atacatatac atttgatgat
     3841 ttaagcatgt tgagtgatgg cgatgtaatt gtttccgttt ctctcactga tattgccgga
     3901 aatattaaca gctcacctta ttcaggaaat gtaacggcag acagaacagt tccgtcagta
     3961 atttctaaaa ccattgctcc gactattgcc aacatgaata ccgaatctgt cacagtcaat
     4021 tttactttca acgagaaggt tgattctttc attaaaaatg atgttacaat gtcaccatcc
     4081 ggaattctga ctgactatgt atgttcaaat tcatctggag atgaacagaa ttacagctgt
     4141 gttttcagta tgagcacggt cggaacatat gaagggctcc ttacattttc agttgaagct
     4201 gttgataagg ccggaaatgc aatggtcccg aatcccgttg aactcggtac tctgacgata
     4261 gacaccatcg ctcccgaact gacctttgat tcaattacgc ctctgactgt aaatgaagat
     4321 accgcttccg ttacagctgt ctttactgct gataa
//
LOCUS       DAOOYB010000244         4345 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_23741_length_4345_cov_6.482751, whole genome shotgun sequence.
ACCESSION   DAOOYB010000244 DAOOYB010000000
VERSION     DAOOYB010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4345
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23741_length_4345_cov_6.482751"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..4269)
                     /locus_tag="PL056_11440"
     CDS             complement(172..4269)
                     /locus_tag="PL056_11440"
                     /inference="COORDINATES: protein motif:HMM:NF019912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HPM47673.1"
                     /translation="MTSPNREWTQDVPGSCPSYCAKRNETQGITNIDGIYWILSKNKW
                     IDVFEVDDSSFSISDKIFTIDNGYNYTFNPDPKNGDIDYEKGEFFVPSGANLVVYGWN
                     GSELIEKNVYEMSSFGVAVHPINGHVFGQADDDGYIYGYKLNKNSSTKICKNTIALHD
                     ISGQRINHEEITNNHWTQGLDFSPNGRYLFYVHDDEFDVNSLYTGIYVYYFDDENFNK
                     LSTSNCFAKSDPIKAILVGFKNIPYDPDVNGVSTARLDELEGIDIGKIGEYEIHQLML
                     HNTAGDNDEKYSVYHYKSPDYDYDTINDIYDNCPFVANSEQYDLDGDSIGDACDSDVD
                     GDGKLNEEDNCPILYDESNRKIFFCEDVDGDEVCDSSVRKLCKTVYENGGINSDAQQS
                     IYNQRWERRPVVFLNGIRNMLIYEEDVRKNFVDNCPGINNPFSYEEGEIMFGIDPADD
                     ITVTGVGALKNTRINTYSSGYYYYWQPDHNLDGNGDACDYGNINNPNDVGTRYSKVTN
                     TDSYTKKGTDPLIRNEYLTIDMKMFGQNENVDDTREQSLRYCNLPLSQEEDWGKDGFC
                     TNTKNSETYKEISHKVRDYSFSHGSDPKPFDERGDTWRELNWTSNPACLSISNSQSSN
                     FYVMNEAVPGAIDLSKICDNRTSPLAGTKKSSTIYWNWRNNFYLDWGLDFFDELYPEE
                     IDIPIPQGRGISHFRFTLSVGVKGQDNNYLSSGLINANHFYNTERYARSARFYDTGIG
                     YYKWEPIDLVEPERMIYFDDWLREIFLREGLFFPEKGWNKFIKWTGSWKDTIKSVPVY
                     IDNFYGAVAEKDGFIFAVDEDQELGTITFKYNHIENSAGWIDAATFSNIPSHLEPAGL
                     SVSDEKMFVLMRNDDTEEYSLFSAGSETLEEIDGLPVMDNITFIEYDGAVYIAGNTGG
                     YMEMYELYQTESGGYALSYVESTQKPSARALFNIHSDETGIYLAGGGNTDWQTIEVKR
                     DVWKFDAENGWQVLNSDTGKDLFKLFMRKEGDNLLMVFQTGLNGNIAKYITFNTSDGT
                     VIEEGFVPVEGAAEVEENSICRTADTSFFWAGFESAGICNKFSAPDYSYFNAGTKIYA
                     VEGFGKYLFAAHGNGIRVYDISNPVSPVLKNNISLYGPVRDLEIEGERVFAATGNGID
                     ILKFNGSSLVIEKHIATYGDSAVMKRYGNYLIIGDGQGLKKLDISTQNIVQQVNTSGD
                     VTTLVIKDGIIHLYDWAGLKRYNAETFVRITTSSSYKSSPELALTIDDRIFVTYSAKV
                     YELTYNGNTPVYTQKTGDALDVRDGYSFDGYGYFAKGNGIRISTMAEIMEAVCGNGVI
                     EPGEVCDGNSVACTTVNSDYDSGTAYCNSTCNGYNESSCETDDGW"
BASE COUNT         1200 a          963 c          738 g         1444 t
ORIGIN      
        1 aggcttcgtc ggacaagtcg tcggaatctt tgtcaggttg aatcatatca atgtcatttt
       61 cagtgtcgct tcccgcaagt atatcagtgt cgctcttcgg gttattgccg ttgcatgatg
      121 tacacgacca gaacaacagc agaagcaata taattaatat gttacgcatg gttaccatcc
      181 atcatcagtt tcgcaactgc tttcattgta tccattgcat gttgaattgc aatatgccgt
      241 tccgctgtcg taatctgaat taacagttgt acatgcaact gaatttccgt cacacacttc
      301 accaggttct atcactccgt ttccgcatac agcttccata atttcagcca tcgtgctgat
      361 ccttatcccg ttcccttttg caaaatatcc gtatccgtca aatgaatatc cgtccctgac
      421 atcaagtgca tcacctgttt tctgcgtgta aacaggggta ttcccgttat aggtgagttc
      481 atacactttt gcactgtatg tgacaaatat cctgtcatca attgtgagtg caagctcagg
      541 acttgattta taactgctcg aagttgttat ccttacaaat gtttccgcat tgtatcgttt
      601 aagtcccgcc cagtcataaa gatgaattat tccatccttg atgacaagag ttgtgacatc
      661 tccggaagta ttgacctgct gaacaatgtt ctgagttgaa atatcaagtt tcttaagtcc
      721 ctgtccgtct ccgattatca agtaatttcc gtatcttttc attacggcac tgtcaccgta
      781 ggtcgcaatg tgcttttcaa tgacaagtga agatccgttg aatttcagaa tatcaattcc
      841 gttacctgta gcggcaaaaa ctctttcacc ttcaatttca agatccctga ccggtccgta
      901 aagtgaaata ttatttttaa gaacagggct gacaggattt gaaatgtcat atactcttat
      961 tccgttaccg tgtgccgcaa acagatactt tccgaatcct tcaacggcat atatttttgt
     1021 tcctgcattg aagtatgaat agtcaggtgc actgaacttg ttacagattc ctgctgattc
     1081 aaatcctgcc cagaaaaatg atgtgtctgc tgtacggcaa atactgtttt cttcaacttc
     1141 agcagctcct tcaaccggaa cgaacccttc ttcaattacc gtgccgtcac ttgtgttgaa
     1201 agtgatatat tttgcaatat ttccattaag tccggtctga aaaaccataa gaagattatc
     1261 gccttctttt ctcataaaaa gtttaaacag gtcttttcct gtgtcactgt tcaaaacctg
     1321 ccagccgttt tctgcatcga atttccacac atctcttttc acttctattg tctgccagtc
     1381 agtgtttcct cctccggcaa gatatattcc cgtttcatct gaatgaatat tgaaaagcgc
     1441 tcttgcagac ggtttctgtg ttgactcaac ataactgaga gcatatccgc cgctttctgt
     1501 ctgataaagt tcatacattt ccatgtatcc gcctgtattt cctgcaatat agacagctcc
     1561 gtcatattca atgaaagtga tgttatccat tacaggaagt ccgtctatct cttcaagtgt
     1621 ttcgcttcct gcactgaaaa gtgaatattc ttccgtgtca tcgtttctca tgagcacgaa
     1681 cattttttca tctgacactg aaagtcctgc cggttcaagg tgtgaaggga tgttgctgaa
     1741 agttgcggca tctatccagc cggcagagtt ttcaatgtgg ttgtatttaa aagtgatcgt
     1801 gccgagttcc tgatcttcat caactgcaaa aatgaatccg tccttttcag caaccgctcc
     1861 gtaaaaattg tcaatataaa caggcacaga cttaattgtg tctttccatg agccagtcca
     1921 ttttatgaac ttgttccatc ctttttcagg gaagaaaagt ccttctctta aaaagatctc
     1981 tctcaaccag tcatcaaaat atatcatcct ttccggctca acaagatcaa tcggttccca
     2041 tttgtaatat ccgatgcccg tgtcataaaa tcttgccgac cttgcatacc tttcagtgtt
     2101 gtaaaaatga ttagcattta tcagtccact ggaaagataa ttattgtcct gtccttttac
     2161 gccgacagaa agtgtaaacc tgaaatggga aatgcctctt ccttgaggga tcggaatatc
     2221 aatttcttca ggataaagct cgtcaaagaa atctaaaccc caatccaaat aaaaattatt
     2281 tctccaattc cagtaaattg ttgagctttt ctttgttccg gcaagagggg atgttctgtt
     2341 gtcacatatc tttgacaaat cgatagcccc tggaacagct tcattcatga cataaaaatt
     2401 tgatgactga ctgtttgaaa tcgaaagaca cgcaggattt gaagtccagt tgagttcccg
     2461 ccatgtatcg cctcgttcat caaagggttt cgggtctgat ccgtgactga atgaatagtc
     2521 tcttacttta tgtgaaatct ctttatatgt ttcagagttt tttgtatttg tgcaaaatcc
     2581 gtctttcccc caatcttctt cctgacttaa cggcagattg cagtatctca aactttgttc
     2641 tcttgtgtca tcaacatttt cattctgccc gaacattttc atgtcaattg taagatattc
     2701 attccggatg agcggatctg tccctttttt agtgtatgaa tctgtatttg tgacctttga
     2761 ataacgggtt cccacatcat tcggattgtt tatattcccg taatcacacg catcaccgtt
     2821 gccatcaaga ttatgatccg gctgccagta gtaataatat cctgaagaat atgtatttat
     2881 tctggtgttc ttcaaagcac cgacccctgt aacagtaata tcatctgcag gatctattcc
     2941 gaacattatc tccccttctt cataagaaaa cgggttgtta attccgggac agttgtcaac
     3001 gaaatttttc ctgacatcct cttcataaat gagcatattt ctgatgccgt taagaaagac
     3061 tacggggcgt ctttcccatc tctgattata aatagactgt tgagcatcac tatttatccc
     3121 accgttttca taaaccgttt tgcagagctt tctgacagaa gaatcgcaaa cctcgtctcc
     3181 gtcaacatct tcacagaaaa atatttttct gtttgactca tcgtaaagaa taggacagtt
     3241 atcttcttca ttcaattttc cgtccccgtc aacatcgctg tcacaagcat ctcctatgct
     3301 gtcgccatcc agatcatatt gttcagaatt tgccacaaaa ggacagttgt cataaatgtc
     3361 atttattgtg tcatagtcat aatctgggga tttgtaatga tagactgagt atttttcatc
     3421 attgtctcca gcggtattat gaagcatcaa ctgatgaatt tcatattctc caatttttcc
     3481 gatatcaatt ccctctaatt catccagtct tgctgttgat acaccgttta catctggatc
     3541 ataaggaata tttttgaatc ctacaaggat tgcttttatt ggatcagact ttgcaaaaca
     3601 gttcgaggtt gacaacttat taaaattttc atcatcaaaa taatacacat aaatccctgt
     3661 gtagagtgaa tttacatcaa actcatcatc atgtacataa aaaagatatc ttccgttggg
     3721 cgagaagtcc aatccctgtg tccaatgatt gttcgttatt tcctcatgat taatcctctg
     3781 tccggaaata tcgtgtagag ctattgtatt tttgcatatt tttgttgacg aatttttgtt
     3841 aagtttgtat ccataaatat aaccatcatc atctgcctga ccgaaaacat gtccatttat
     3901 tgggtgaaca gcaacaccaa aagagctcat ttcataaaca ttcttttcaa taagctcact
     3961 gccattccac ccataaacga caaggtttgc accactaggg acaaaaaact cccctttttc
     4021 atagtctatg tctccgtttt tcgggtcagg attaaatgtg tagttataac cattatcaat
     4081 agtaaatatt ttgtcagaaa tggaaaaaga ggaatcgtca acttcaaaaa catctatcca
     4141 tttgtttttg gacaatatcc agtatatccc atcaatattt gtaatgcctt gtgtttcatt
     4201 tctttttgca caataagaag gacacgagcc tggcacatct tgagtccact ctctattagg
     4261 agaggtcatt ttatttccaa aaacaaattc ataagagcca attgcaaaac tattttcgta
     4321 aaaaagtaca ggatgtcaac ttctg
//
LOCUS       DAOOYB010000245         4292 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_24166_length_4292_cov_8.728818, whole genome shotgun sequence.
ACCESSION   DAOOYB010000245 DAOOYB010000000
VERSION     DAOOYB010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4292
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24166_length_4292_cov_8.728818"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..764)
                     /locus_tag="PL056_11445"
     CDS             complement(99..764)
                     /locus_tag="PL056_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47674.1"
                     /translation="MKKLIILLIVLISFYNLTAEEENMFGDTSEPVTNEDKLEENEGK
                     ETNAAPAETKPAKGGNKFQQFVHRSPNKKALAGIKFGPEIYHTNYTKNNSLFNDRVDY
                     GFHTDLFFEYNFIRNFGLQAELNFGGGYFGFFRLPIIAQFNLPINDMFWLNFGLGPYL
                     AAGYWNGFDLGLIAKAALEINTKVGVFVADIRYTSSLYGLKYDWYFGSFAILVGYAVP
                     LPF"
     gene            912..2894
                     /locus_tag="PL056_11450"
     CDS             912..2894
                     /locus_tag="PL056_11450"
                     /inference="COORDINATES: protein motif:HMM:NF012632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47675.1"
                     /translation="MKKYLFFICLIIFFVSCNSEKKEVNDIDLNDHENGDTALNDEHD
                     EEIDADLNDESFDEDNAVDPDVVENNDGDTEIDEDGEETENDGDDDEDVIDDGRCIQI
                     NIGNKYDFGTITHGNTASSHISVCNTCGFAVIPDALKDNGFFGKCGSISITDLEIDGN
                     PVDTSSGFDILLAMSLQAGLCAEMDAVYDSGYGEYYPEILHCELKGSFSDGFDYSILF
                     TGTSGENSEIDQDECPALKDLCYIDGKCYSNGAHDPVNLCRICDPASSGTWSYKNENN
                     ICDDGLINTTGDKCNDSGVCSGTVDPEGVPPMAGIDAGWNYTCALGENGKAYCWGMNN
                     SGQAGPGSSEFKILSPFEITVSGGFSKLSAGYSHVCALSNGEEVFCWGSNLQGQLGKE
                     SSDPVPLEAVSGISGVESVSAGGYHSCALLDDGTVKCWGNNEHGTLGNGTTEDSAAPV
                     SVNGLQNVKAISAGFDHVCAVIDDGTVKCWGSNEYSQLGSAGVSFSSTPVEIGGISSA
                     ADISCGRYHSCILLLNGKVECWGESDYELLGNYFTWNSEYPVESMITGASTTIDAGDS
                     HTCAVLDDKSSVCWGYNEYGQLGTGYASGGSFIKYPVDVRNISNGIKDIACGTSHTCA
                     VFTDGSVRCWGSGSYGELGTGNELPSLIPVEISWND"
     gene            2936..3736
                     /locus_tag="PL056_11455"
     CDS             2936..3736
                     /locus_tag="PL056_11455"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HPM47676.1"
                     /translation="MRVAIIQNNPVWGEKEKNINELIRLMNKEKADIYILPEMAYTGY
                     QITSKEEIENLADTIDSENISRFAKFSKEQDCAVIVGFPEKAADGHFYNSSVMITPEG
                     DKHIYRKTHLFYKEKLFFAPGNTGFNVFEWRGVKVGLAICFDWYFSESFRTLALIGAD
                     IIAHCSNLVMPHCQTVDYARAIENRIFIATANRIGSEARDGEKLTFTGQSVLVSPKGE
                     YLIKAPAEETGCFTAQIDTNLSRNKKLNEFNDVLADRRELFYWKHPVK"
     gene            complement(3752..>4292)
                     /locus_tag="PL056_11460"
     CDS             complement(3752..>4292)
                     /locus_tag="PL056_11460"
                     /inference="COORDINATES: protein motif:HMM:NF019297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47677.1"
                     /translation="TPFTCEIDLDFKEHLIYGENNIEINFTGEGSMMYQATATWYIPG
                     EDDTSTGPLTIDVTYDKTSLNVNDTVGVKVDIKNVSDAGVSIVLASIGIAPGFTLIPA
                     QLDTAVTEGTYLQKYETTPRQVILYINHIAKGATATFNFELLADYPMEGATGESSVNP
                     YYNPEQKHNDNSQKLTVIE"
BASE COUNT         1293 a          756 c          983 g         1260 t
ORIGIN      
        1 ttttcataat tccccccaaa gaaagttaaa aagttccgca gagaaattac aactatttta
       61 gtaaaaaagc aaatgaatta tctgttaagg gaaaattttc agaaaggaag cggaaccgca
      121 taaccgacaa gaatagcaaa tgaaccgaaa taccagtcat attttaaccc gtaaagactg
      181 gatgtgtatc ttatatcggc aacgaaaaca ccgacctttg tattaatttc aagtgctgcc
      241 tttgctataa gaccaagatc aaatccattc cagtatcctg cggcaagata tggccccaat
      301 ccgaaattga gccagaacat atcatttatc ggaagattga attgtgcaat tattggaaga
      361 cggaaaaaac cgaaataacc gccgccgaaa ttcaattctg cctgaagacc gaaattcctg
      421 ataaaattat attcaaagaa aagatctgta tggaagccgt agtcaacacg gtcattgaag
      481 agagaattat tcttagtata gttcgtgtga tatatctcag gaccgaattt gatgccggca
      541 agtgcctttt tattgggtga tctgtgaacg aattgctgaa atttgtttcc gccttttgcc
      601 ggttttgttt ctgcaggagc ggcatttgtt tccttccctt cgttttcttc gagcttgtct
      661 tcgtttgtaa ccggttcact tgtgtctccg aacatgttct cttcttcagc agtaagattg
      721 tagaaactga taagaacgat cagtaaaata atcaattttt tcataaaacc ctcccaaagt
      781 ttataataaa caacaaacgg actgatatat aagcacaaaa aaagaaaagg aaaggtttta
      841 atgcgaaaat tgcaaaaaaa atatattatt ctattatcaa accacgaact gtttttttat
      901 gggggaatat tatgaagaaa tatctatttt ttatctgttt aattattttt tttgtttcct
      961 gcaacagtga aaagaaagag gtgaatgaca ttgatcttaa cgatcatgaa aatggagaca
     1021 ccgctttaaa tgatgaacat gatgaagaaa ttgatgcaga cctaaatgat gagtcctttg
     1081 atgaagataa tgcggttgac ccggatgttg ttgaaaataa tgatggtgat acggaaattg
     1141 atgaagacgg cgaagaaaca gaaaatgatg gagatgatga tgaagatgtc atcgatgatg
     1201 gaagatgtat tcaaatcaat atcggaaata aatatgattt tggaacgata acccatggaa
     1261 ataccgcttc aagccacatc tctgtctgta acacatgcgg ttttgctgta attccagatg
     1321 cacttaaaga caatggtttt ttcggcaaat gtggaagcat ttccataaca gatcttgaga
     1381 ttgatggaaa tcccgttgat accagttcgg gatttgacat tcttcttgcc atgtctcttc
     1441 aagccggatt gtgtgcggag atggatgctg tctatgattc aggttacggg gagtattatc
     1501 cagaaattct tcattgcgaa ttgaagggtt cgttttctga cggatttgat tattccattc
     1561 tgttcacagg aacatccggt gaaaattcag aaattgacca ggatgaatgc cctgcattga
     1621 aagatctttg ttatattgac ggtaaatgtt attcaaatgg agctcacgat cctgtaaatt
     1681 tatgcagaat atgtgatccg gcttcgtctg ggacatggag ctataagaac gagaacaata
     1741 tatgtgacga tggtttaatt aatacgacag gcgataaatg caatgattcc ggtgtctgtt
     1801 cgggaacagt tgatcctgaa ggggttcctc ctatggctgg aattgatgca ggatggaatt
     1861 atacatgtgc tttaggagaa aacggaaaag catactgctg ggggatgaat aactctggtc
     1921 aggcaggtcc cggatcatct gaattcaaga tattgagtcc ttttgaaata actgtttcag
     1981 gcggattcag caagttaagc gcgggctatt cacatgtctg tgcactttca aacggtgaag
     2041 aggtgttctg ctggggaagc aacctgcagg gtcagctcgg gaaggaaagt tccgatcctg
     2101 ttccacttga agctgtgtca ggaatatccg gagtggaaag tgtttcggca ggcggttatc
     2161 attcatgtgc tcttcttgat gacggtactg taaaatgttg ggggaataat gaacacggaa
     2221 ctctcggcaa cggaacgact gaagatagtg cggcacctgt ttcagtcaat ggtcttcaga
     2281 atgtaaaagc tatctcagcc ggttttgatc atgtttgtgc tgtcattgat gatggaactg
     2341 taaaatgttg gggatctaat gagtatagtc agcttggatc cgccggtgtt tcgttcagtt
     2401 caacacctgt cgagatcgga gggatcagca gtgccgcaga tataagctgt ggacgatacc
     2461 acagttgtat acttctttta aatggcaagg tggagtgctg gggggaaagt gactatgaac
     2521 ttcttggaaa ttatttcaca tggaattctg aatatcctgt cgaatctatg ataaccggtg
     2581 cttcaacaac tattgatgcc ggtgatagcc atacctgtgc ggtgcttgac gataaaagct
     2641 cggtatgctg gggatataat gaatatggac agcttggaac aggatatgct tcaggtggaa
     2701 gttttattaa atatccggta gatgtcagaa atatttctaa cggaataaaa gatatcgctt
     2761 gcggcacttc gcatacttgc gcagtgttta cagacggttc tgtaagatgc tggggaagcg
     2821 gttcttatgg ggaacttggt acaggaaacg aattgccttc tttaattcct gttgaaatat
     2881 catggaatga ttgatctgta atttatattg gaatcatttt tttcgggagt tgacaatgag
     2941 agtcgcaatt attcagaaca acccggtctg gggagaaaaa gagaaaaata tcaatgagtt
     3001 gatcagattg atgaacaaag agaaagcaga tatttatata cttcctgaaa tggcttacac
     3061 tggatatcag atcacttcaa aagaagaaat tgaaaacctt gccgacacta ttgattcaga
     3121 aaatatcagc cgttttgcaa agttttcaaa agagcaggat tgtgcagtga tagtcggctt
     3181 tcccgaaaaa gcggcagacg gtcactttta taactcaagt gtgatgataa ctcctgaggg
     3241 agataagcac atttacagaa aaacacacct tttttataag gaaaagcttt tttttgctcc
     3301 cggcaacacc ggctttaatg tgtttgagtg gagaggagtt aaagtggggc ttgcaatatg
     3361 ctttgactgg tatttttctg aaagtttcag aacacttgcg ttaattggtg ccgacataat
     3421 agcgcattgc tcaaatcttg ttatgcctca ctgccagaca gttgactatg ccagagcaat
     3481 tgaaaacagg atctttattg caactgcaaa ccgcatcgga tctgaagcaa gagacggcga
     3541 aaaactcact ttcacaggcc aaagtgttct tgtttcaccc aaaggagaat atctcataaa
     3601 agctccggct gaagaaacag ggtgctttac ggctcaaatt gacacaaatc tatcccgcaa
     3661 caaaaaactt aatgaattca atgatgtgct tgcagataga agagaattgt tctattggaa
     3721 acatcctgtg aaatgatctg ataacgggag tttattcaat aacagtcagt ttctgagaat
     3781 tatcgttatg tttctgttcg gggttgtagt aggggttgac tgatgattcg cctgttgccc
     3841 cttccatcgg ataatcggca agaagttcaa aattgaatgt tgcggtcgct ccttttgcga
     3901 tgtggttgat atagaggatg acctgtcttg gtgtagtttc atatttctgg agatatgtcc
     3961 cttcagtaac ggcggtgtca agctgagccg gaataagtgt gaatccgggt gcgataccta
     4021 tgcttgcaag gactatgctc actcctgcat cactgacatt ttttatatca actttaactc
     4081 cgaccgtatc gttcacattc agagatgtct tgtcataggt gacatcgatc gtgagcggac
     4141 ctgtcgaagt gtcgtcttcg cccggaatgt accatgttgc agttgcctga tacatcatac
     4201 tcccttcacc tgtgaaattg atctcgatat tgttctcacc gtaaatgagg tgctctttga
     4261 aatcgagatc aatttcacag gtgaagggag ta
//
LOCUS       DAOOYB010000246         4260 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_24417_length_4260_cov_5.134126, whole genome shotgun sequence.
ACCESSION   DAOOYB010000246 DAOOYB010000000
VERSION     DAOOYB010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4260
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24417_length_4260_cov_5.134126"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="PL056_11465"
     CDS             complement(<1..213)
                     /locus_tag="PL056_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47678.1"
                     /translation="MNTRISKYFFLAVIMISAGFYSLNLDAQCVVGRSAENQGSTYNS
                     VSVAPQWDTEHYNNITAGDCVEYNVLA"
     gene            complement(225..977)
                     /locus_tag="PL056_11470"
     CDS             complement(225..977)
                     /locus_tag="PL056_11470"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HPM47679.1"
                     /translation="MKTNFFKMFFFLLVIPWVVTSCFNEIPVEEDKNSTYQNSEQENG
                     KVPLENRLNSSKTGALTTKLFVPYSKLDSARSYNFPNIQSWQMISTGQKKCYDNFGEI
                     NCPDVDDPEYGGSGAVSYGPRVYIPNGDGTVKDGVTNLLWQSGYITSKTWYEAKNYCD
                     NLTLAGKRWRLPLTHELRSLVHYGVNAPSIDGSAFPDTPSDWFWSSKHTNYSTMNAGT
                     ETVASWIISFIDGSVEYTGRDNYYSVRCVAVE"
     gene            complement(991..1389)
                     /locus_tag="PL056_11475"
     CDS             complement(991..1389)
                     /locus_tag="PL056_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006965159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPM47680.1"
                     /translation="MKGFTLIELMIVLAIIAILSAVAIPLYDGYAERSAKAELDQELA
                     NFAAVQEDYFNSYRKYSGNLAELADYYGVTVQGTRFVIKNLTVGSGAGSYSVDGYVCY
                     SVPVASCGAGANDYYCKLQSGQEQPVCTKN"
     gene            complement(1391..1891)
                     /locus_tag="PL056_11480"
     CDS             complement(1391..1891)
                     /locus_tag="PL056_11480"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPM47681.1"
                     /translation="MRNSFRKKGFTLPEVLIVLAIISILAFVSIPIFQGFADNGKIQE
                     LKADLIKLATSQENYYSAKGRFASRIEDLEKFGYVPPVNNKKSFFTGVIIDEDLGMKY
                     WVAGNYKITTKATAYAECWVYLGENIKTGEDDPFVNLYNENKSESRELTCAFCPKIKN
                     VCKYGE"
     gene            complement(1891..2700)
                     /locus_tag="PL056_11485"
     CDS             complement(1891..2700)
                     /locus_tag="PL056_11485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47682.1"
                     /translation="MNFYQKKVFAVTILLLLFYGNVFADKETDKKGGVLDSYVEFPVI
                     VFVGQITNLTPYSIIFENRYAPTKATNDKINKLMKGYASSIDPMSHGFAKALKVVTVV
                     DNKMRASFYLDNITSVFADDMQSYMKFLPLYYIFKHNIDTLKLPKGHIEKLKKLSLNY
                     YMTLPMAEKIMNNSWFVGVFRIDPDSSKLIYQSSNYYSDIDELFTDDFAKEYMVDKIV
                     KAGMVMSVTKAKIGGRESEWVKEKLKNRKEKKLDDKNKFYNKTPDTSGESN"
     gene            complement(2713..>4260)
                     /locus_tag="PL056_11490"
     CDS             complement(2713..>4260)
                     /locus_tag="PL056_11490"
                     /inference="COORDINATES: protein motif:HMM:NF017388.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilC/PilY family type IV pilus protein"
                     /protein_id="HPM47683.1"
                     /translation="AKTTIRPLLMGAIAHSKPVAVYYETSEETRIFVGANDGMLHSFD
                     QDGDEVFAYVPSPSMPALRAISHSSSAIVFRSSVDGPIELFHLDLNNDGMITSGEKAY
                     LIFGYRRGANAYTVLDISEMDNPRLVQHIGTEGQSWAKPLTFMSNGSYYLLVPGGYDT
                     CFDADIPSQASCGVTNGALDPKGNEIKIYRFDESKKDGTNAFEFVKEYKPSDETGYAD
                     WLRASFSSDPIAISTLDSLNKGAELVYLIDVTGTVFRINVSSANYNNWNVDVVYAQRD
                     SAYDVQTKWIVLDHQQRVYANFAIYPYGTTFPSQNGSNIVPVPIVTGNLVNPLATTLN
                     GMVLFYDDKSLNPIAPTINTGGTDFPNVSSPSEVEGETYYATAKGWRIPFDKNHDTNG
                     LFMEKGITRPLIYYDRWDSNTYTLVWNTYLPAKWTECRNFGTSLNYARDLWTGKKPQL
                     GPYSDFDDDVYWSASKSISIATEIGVLATKKGESIVFSSGDDLFATKPVEPPNNSAKI
                     IKWYELY"
BASE COUNT         1278 a          900 c          712 g         1370 t
ORIGIN      
        1 cgcaagcaca ttgtattcta cacaatctcc ggcagtaatg ttattataat gctcagtatc
       61 ccattgaggt gcaactgata cagagttata tgtagaaccc tgattttcag cacttcttcc
      121 tacaacacac tgtgcatcca gattaagaga ataaaaaccg gcagaaatca taataactgc
      181 cagaaagaaa tatttgctta ttcttgtatt cataatttcc tcctttattc tactgctaca
      241 cagcgaacgc tgtaataatt atctcttccc gtatattcaa ccgacccgtc aataaaactg
      301 ataatccatg aagccacagt ctctgtaccg gcattcatag tgctgtaatt tgtatgttta
      361 gaagaccaga accagtcaga cggagtatcc ggaaaagctg aaccgtcaat tgagggagca
      421 tttacaccat agtggacaag agatcttaac tcgtgcgtta acgggagtct ccatcgcttg
      481 ccggccaaag taagattgtc acaataattt tttgcttcat accaggtttt actggtaata
      541 tatcccgact gccacaaaag attggtaacc ccgtctttaa cagtgccatc tccgttaggg
      601 atataaactc tgggaccgta agaaactgca ccactgccgc catattccgg atcatcaaca
      661 tccggacaat tgatctcacc aaaattatca taacattttt tctgtccggt agatatcatc
      721 tgccaactct gtatgtttgg aaaattataa gatctggcgg aatccagctt actgtaaggg
      781 acaaataatt tcgtggttaa tgcacctgtt tttgaactgt tgagtctgtt ttccaaagga
      841 actttgccgt tttcctgttc agaattttga taagtgctgt ttttatcctc ttctacaggt
      901 atttcattaa aacaggatgt cacaacccaa ggtataacaa gcagaaaaaa gaacatttta
      961 aaaaagtttg ttttcatttt atcctccatg ttaatttttt gtacatacag gttgttcctg
     1021 cccgctttgt aacttacaat aataatcatt cgctccggca ccacatgatg cgacaggaac
     1081 agaataacag acataaccat ctacagaata agatcctgcc cctgacccga cagttaaatt
     1141 cttaattacg aatcttgttc cctgcactgt tacaccataa taatcagcaa gctctgccaa
     1201 attgccagaa tatttcctgt aactgttgaa ataatcttcc tgaacagctg caaaattggc
     1261 aagctcctga tccagttcag cttttgcaga tcgctccgca taaccatcgt ataaaggtat
     1321 agcaacagca gaaagaatcg caataattgc aagcactatc atcaattcaa tgagtgtaaa
     1381 acctttcatg ttattcccca tatttacaaa cattttttat tttaggacag aaagcgcatg
     1441 taagttctct gctttcactc ttattttcgt tgtataaatt cacaaaagga tcatcttctc
     1501 ccgtttttat attttcgccc agataaaccc agcactcagc atatgcagtt gcttttgtgg
     1561 taattttata atttccggcg acccaatatt tcattccaag atcctcatca ataattactc
     1621 ctgtgaaaaa acttttttta ttgttaacag gcggaacata accaaacttt tcaagatcct
     1681 cgatccttga ggcgaaacga cccttggctg aatagtagtt ttcctgtgaa gtggccagtt
     1741 taattagatc tgctttcagc tcctgtattt ttccattatc agcaaaaccc tggaaaatag
     1801 gaatagatac aaaagccaat attgaaatta ttgcgagaac aattaatact tcaggaagtg
     1861 taaatccttt tttgcgaaaa ctatttctca ttaatttgat tctcctgatg tgtccggtgt
     1921 tttattataa aatttatttt tatcatcaag ctttttttct tttctgtttt tgagtttttc
     1981 cttaacccat tctgattccc ttcctcctat ttttgctttt gtcacactca taaccattcc
     2041 ggccttaact atcttgtcta ccatatattc tttagcaaaa tcatctgtaa aaagctcatc
     2101 aatatcagaa taataattag aactctggta aatcagcttg gaagaatcag gatcaatcct
     2161 gaaaactccg acaaaccagg aattattcat tattttctct gccattggca gcgtcatgta
     2221 gtaattcagt gaaagttttt ttaatttttc tatatgtcct ttcggaagtt taagcgtgtc
     2281 aatattgtgc ttaaatatat aatacaaagg aagaaatttc atatagctct gcatatcgtc
     2341 cgcaaacaca gatgtgatat tatctaaata aaatgatgct ctcattttat tatcaacaac
     2401 tgtcacaact tttaaagctt ttgcaaaccc atggctcatt ggatcaatgc ttgatgcata
     2461 tcctttcatt aacttgttta ttttgtcatt ggtagctttt gttggagcat atctgttctc
     2521 aaaaattatt gaatatggtg tcagatttgt aatctgtccg acaaaaacaa taacaggaaa
     2581 ctccacataa gaatcaagaa cacccccttt tttatcagtt tctttgtcgg caaaaacatt
     2641 gccgtaaaat aaaagcagca atatagtaac tgcaaatact tttttctgat agaaattcat
     2701 tccttatctc ctttaatata actcatacca tttaattatt ttagctgagt tgtttggcgg
     2761 ttctacgggc tttgtcgcaa ataaatcatc tccgcttgaa aagacaatgc tttcaccttt
     2821 tttagttgcc agaactccaa tttctgtggc aatactgatt gattttgaag cactccaata
     2881 tacatcatca tcaaaatcac tgtaaggtcc caactgcggt tttttcccgg tccagaggtc
     2941 tctggcataa ttcagactcg taccaaaatt cctgcactct gtccattttg cggggagata
     3001 ggtattccag acaagtgtat atgtgtttga atcccatcta tcataatata tcagtggacg
     3061 ggttatacct ttttccataa aaagaccgtt tgtgtcatgg tttttatcaa aagggattcg
     3121 ccatccttta gccgtagcat aataagtctc accttcaact tctgagggag aagaaacatt
     3181 cggaaaatct gtacctccgg tattaatcgt gggagcaatc ggattcagtg acttatcatc
     3241 ataaaaaaga accataccgt ttagtgttgt ggccagggga ttcaccagat ttcccgtaac
     3301 tataggcacc ggtacaatgt tggaaccgtt ctgggacggg aatgtggttc cgtaagggta
     3361 tatcgcaaag tttgcataaa ctctctgctg atggtcaagc actatccatt ttgtctgtac
     3421 atcgtatgca gaatctctct gtgcataaac gacatcaaca ttccagttgt tgtaatttgc
     3481 cgaacttaca ttgattctga aaactgtacc tgttacatca ataagatata ccagctcagc
     3541 tcctttattt aatgaatcaa gtgtgctaat ggcaatcgga tctgatgaaa aagaagctct
     3601 aagccaatcg gcataaccgg tttcgtcaga tggtttatac tctttaacaa attcaaaagc
     3661 attggtccca tcttttttgc tttcatcaaa tctgtaaatt tttatttcat ttcccttggg
     3721 atctaaagca ccgtttgtta caccacaaga agcttgtgat ggaatatcag catcaaaaca
     3781 ggtatcatac cctcccggaa caagtaaata atacgagccg ttagacataa aagtaagtgg
     3841 tttcgcccaa ctctgtcctt ctgtcccgat atgctgtaca agacgaggat tatccatttc
     3901 tgaaatatca agaacggtat atgcatttgc tcctctgcgg tatccaaaga taagatacgc
     3961 cttttctccg gaggttatca ttccatcgtt atttaaatca aggtgaaaca gttcgatcgg
     4021 accatcaaca gaagatctga aaactattgc ggaactggaa tgacttattg ctctaagagc
     4081 cggcatagaa ggagagggaa catatgcaaa aacttcatct ccatcctgat caaagctatg
     4141 aagcattcca tcgttggctc cgacaaaaat tctcgtttct tctgatgttt cataataaac
     4201 cgctaccggt tttgaatgag caatggctcc catcagaagt ggcctgatcg tggtttttgc
//
LOCUS       DAOOYB010000247         4253 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_24481_length_4253_cov_7.358504, whole genome shotgun sequence.
ACCESSION   DAOOYB010000247 DAOOYB010000000
VERSION     DAOOYB010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4253
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24481_length_4253_cov_7.358504"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            135..467
                     /locus_tag="PL056_11495"
     CDS             135..467
                     /locus_tag="PL056_11495"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HPM47684.1"
                     /translation="MGRASVGSAAEVDGQIDSASMNKILRGSGAAIKRCYDKALLVNP
                     TLKGKVALSIMINTQGRVEAADVVEDTIGDKSVSSCIQGVISRLRFPKPDGGPVSFVF
                     PVTFAPSN"
     gene            484..1206
                     /locus_tag="PL056_11500"
     CDS             484..1206
                     /locus_tag="PL056_11500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47685.1"
                     /translation="MLNFKVIFIAAVLVLFFFELSAENKESVSTLDKKGEISKSDIES
                     LKKESKEKVRRISEIELLLAKRVQDAVKEKDIKWKVCLDNYVGALKGVAVSALNAGEK
                     IPELIEAEKTDDAQSQMILLRGLADSAEKTLSEAESCERQLTNVNAESSTTKESNKKL
                     TGETLKDSVNDAMGVGFSDKFVTETDKSVVSGSDIADASGVDSSDVNSESNSSSGEKS
                     DSQKEALGNIDKPEVVDASPTK"
     gene            1243..2673
                     /locus_tag="PL056_11505"
     CDS             1243..2673
                     /locus_tag="PL056_11505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47686.1"
                     /translation="MFNNGIKSVSLVFVSVLVLFCTQSVVAQSNEAAKKGFKITKDTI
                     LNVGLSITPEYESNITKASENSTSTDNLTGTTENFETISDMVLHYSPNLRIKLDDQSK
                     TVGMSLFFDYNHYLGLEDKSASKKLSDLDIKSELLGEFNKNGFLIFDFKNTFGRTATP
                     DGQEISGKHKNILENFIMGLGFKNIEDTLYGKIQAGVDFNYLEQSKDNKVYRDYNYVS
                     FVGDIFGRWKFLPRTMIFMKAAFRYQDFYESSVRDDSRSMPLNVFAGLMGQMTPHIST
                     KISGGYSANFGKETRHDYNANAELIFKYNDSTFMNVGYLKSMRPSAYFQYYSTHRAYL
                     NFKQKFARVFLAGVDSSYSYISFGKSVEFNGVKYAIDLSNTDNSINVYNSSDDRGAAI
                     TSIIPTADRNDHLLLLNPYISYSILSWLGLKLSYEMEYRKTDYYKEIQAVYTDASAPA
                     TNYNRTVNTHFDYINHRVLFTIALDY"
     gene            2677..3315
                     /locus_tag="PL056_11510"
     CDS             2677..3315
                     /locus_tag="PL056_11510"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HPM47687.1"
                     /translation="MKIFSLVSICIIFQMFFLSCTIRVGGNDKKQSSNGSIVSESNGA
                     SDGEILSYIGPGDMLKIQVYGEKELTGTYQVSPEGSIMFPFIGEVKVEGLNNFSLAAK
                     ISEMLKDGYINDPQVTVLVEEFVSKRIFVLGQVKKAGSFPIRTRMSVIEAISLSGGFT
                     DFADISNVVVTRKGKDEKEKRFVIDIKEIVNGTKENFYLNAGDIIFVRERFF"
     gene            3315..>4253
                     /locus_tag="PL056_11515"
     CDS             3315..>4253
                     /locus_tag="PL056_11515"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HPM47688.1"
                     /translation="MHEREIELKEILVLLKRHMYLFSSVIIVVMTLTIVHIYRSQKIY
                     SSTASIEIEPKAANVLGDRMEIVSTGSQGYYWSNKEYYATQYEIIQSRSVAEKVLELI
                     PGENILEYLGLDISKIEEDKLKDIDPVNIILGKLTVSPQKNSNIVKISIEDKDPEKAA
                     FLANSFANAYIDFNLEKKYFATKDAARWLSDQSINLKKSLEDSEKMLFDFKQNNNVLA
                     TTFEAKQAMLATRIEKFTNTLTDQEIRKNAVSAKIEEYSKIDLEDPNESFLKEASKDN
                     QLINNLKMKYIEVVSKLDESEKYYGEKYPKVLSLSTE"
BASE COUNT         1442 a          651 c          940 g         1220 t
ORIGIN      
        1 gagaagcaac gagttcatga cagagaagca gaaaaggtga tgacggagca aatgaaactg
       61 caaaaattga caatttaaat gttaaatcgt ctgatggaaa gattgatata aaaggaaggg
      121 ctgagaaggc tgttgtggga agagcaagtg tcggatctgc cgcggaagtg gacggtcaga
      181 tagattccgc cagcatgaat aagatactta gaggtagcgg tgcggcaata aaaagatgtt
      241 atgataaagc gctcttagta aatcctacct tgaaaggtaa ggttgcttta agtataatga
      301 taaatactca gggtcgtgtt gaggcggccg atgttgttga agatactatt ggagacaagt
      361 cagtatcttc ttgtattcag ggtgtcataa gcaggttgcg ttttccgaaa ccggacggcg
      421 gacctgtaag ttttgttttc cctgttacgt ttgctccttc aaattgattt aatggagggt
      481 gccatgttaa attttaaagt tattttcatt gcggctgtat tagttctgtt cttttttgaa
      541 ctgtcggctg aaaacaagga atctgtttcg acactggata aaaaaggcga gatttctaaa
      601 tccgatattg aatcattgaa aaaagaatct aaagaaaaag ttcgcagaat atcagaaata
      661 gagttgcttc tggcaaagag agtacaggat gctgtaaaag aaaaagacat aaagtggaaa
      721 gtttgtctgg ataattatgt gggtgcattg aagggtgtgg ctgtcagtgc attaaatgca
      781 ggagaaaaga ttcctgaact tatagaagca gaaaaaacgg atgatgctca gagtcaaatg
      841 atacttctta gagggctcgc agattctgcg gaaaaaacac tttcagaagc tgaatcatgt
      901 gaaagacaat taacaaatgt caatgctgaa agcagtacca caaaggaaag taacaagaag
      961 ctaacgggag aaacattaaa agatagcgta aatgatgcta tgggagtggg attctcagat
     1021 aaatttgtta ctgaaactga caaaagtgtt gtttctggat cagatattgc ggatgccagt
     1081 ggggtagatt ccagtgatgt caactccgaa tcaaacagtt cttcaggtga aaaaagcgac
     1141 agtcagaagg aagctctggg caacattgat aaacctgaag tagttgatgc gagtcccaca
     1201 aagtgagaat agtgaaattt gaatatcagt aattaggcga atatgttcaa taacggtata
     1261 aaatctgtaa gtttggtctt tgtgtcagtt cttgttttat tttgcacaca gagtgttgtt
     1321 gcacagtcaa atgaagcggc caaaaaaggt ttcaagataa caaaagatac gatcttaaat
     1381 gtcgggcttt caattactcc ggaatatgaa tccaacatta caaaggcatc cgagaattcg
     1441 acatcaacag acaatttaac cggaacaaca gaaaattttg aaaccatatc agacatggtt
     1501 cttcattaca gtcctaattt gcgaattaaa cttgatgacc agagtaagac ggtcgggatg
     1561 tctttattct ttgattataa tcactatctt ggccttgaag acaagagtgc ttccaaaaaa
     1621 ctgtcagatc ttgatataaa atcggagttg ttgggggaat ttaataagaa cggattttta
     1681 atttttgatt ttaaaaatac atttggcagg acagctactc ctgatggaca ggaaatatca
     1741 gggaaacaca aaaatattct agaaaatttt attatgggtc ttggatttaa gaatattgaa
     1801 gacacgcttt atggaaaaat ccaggcaggt gtagatttta attatcttga acagtccaaa
     1861 gacaacaagg tctatagaga ctacaactat gtttcttttg tgggtgatat tttcggaaga
     1921 tggaagtttc ttcccagaac gatgatattt atgaaggcgg ctttcagata tcaggatttt
     1981 tatgaatctt ccgtaaggga cgattcaagg tcgatgcctc tgaatgtctt tgccggcctg
     2041 atggggcaga tgacaccaca tatttccaca aagatatcag gtggttattc tgctaatttc
     2101 ggcaaggaaa caagacatga ttacaatgcc aatgcagagc tcatttttaa gtataacgat
     2161 tctactttta tgaatgtcgg gtatttaaaa agcatgagac cctcggctta tttccaatat
     2221 tattctacgc accgtgctta tttgaacttt aagcagaagt ttgcaagggt tttccttgcc
     2281 ggtgttgatt caagttattc atacatatct tttggaaaaa gcgttgaatt caacggtgta
     2341 aaatatgcga tagatctgtc aaatacagat aacagcatca atgtttacaa ttcctctgat
     2401 gacagaggtg cggcgataac ctcaataatt cccacagcag atagaaacga tcatcttttg
     2461 ttattgaatc cttatatttc atacagtatt ttaagttggc tgggtttaaa actttcatac
     2521 gaaatggaat atagaaaaac agattactat aaggaaattc aggcagttta tacagatgct
     2581 tctgcacctg caacaaatta caacagaacg gtcaatacac atttcgatta tattaatcac
     2641 agagtgcttt ttacaatagc tctggattat taaattatga aaatcttttc gcttgtttca
     2701 atatgcataa tttttcagat gtttttcttg tcctgtacta ttcgtgtggg tggtaacgac
     2761 aaaaaacaaa gttcaaatgg aagcatcgtt tcagaaagca atggtgcatc ggacggtgaa
     2821 atactttctt acatcgggcc gggcgacatg ttgaaaatac aggtttatgg tgaaaaggaa
     2881 ctgacaggga cttatcaggt aagccctgaa ggatcaataa tgttcccatt tataggtgag
     2941 gtcaaagttg aaggcctcaa taatttttct ctggctgcga aaatttctga aatgcttaaa
     3001 gacgggtata ttaacgatcc gcaggtaaca gttctggttg aggagtttgt aagtaaaagg
     3061 atatttgttc tgggtcaggt taaaaaggca ggaagttttc cgataaggac aaggatgagc
     3121 gtcattgagg caataagtct ttccggtggt tttacagatt ttgctgatat aagtaatgtc
     3181 gttgtaacta gaaagggaaa ggatgagaag gaaaaaaggt ttgtaattga cattaaagag
     3241 atagtaaacg gtacaaaaga gaatttctac ctcaatgcgg gtgacattat ttttgtaagg
     3301 gaaaggttct tctgatgcac gaaagagaga tagaacttaa ggaaatactg gtacttctca
     3361 aaaggcatat gtatcttttc tcttcagtga tcatagttgt gatgactctt acgatcgtac
     3421 atatttacag atctcagaaa atatattcgt caacggcatc gattgaaatt gagcctaaag
     3481 cggcgaatgt tcttggtgac agaatggaga tcgtttcaac cggatctcag ggttattact
     3541 ggtcaaataa ggaatattat gcgactcaat acgaaataat acagagtcgc tctgttgctg
     3601 aaaaggttct tgaactaatt cccggagaaa atattcttga gtatcttgga cttgatattt
     3661 caaagatcga agaggataag cttaaggata tagatcctgt aaacattatt ttaggaaagt
     3721 tgacggtttc acctcagaag aacagcaata tagtaaaaat aagcattgaa gacaaggacc
     3781 ctgaaaaagc tgcttttctt gcgaattctt ttgcgaatgc ctatatagat ttcaatctgg
     3841 aaaaaaaata ttttgccaca aaagatgccg caagatggct ttccgaccag agtataaatc
     3901 ttaaaaagag tcttgaagat tccgaaaaga tgctttttga ttttaagcag aataacaatg
     3961 ttcttgcaac aacttttgaa gcgaagcagg caatgcttgc gacaaggata gagaagttta
     4021 cgaacacttt gacagatcag gaaataagaa agaatgccgt gtctgcaaaa atagaggaat
     4081 acagcaaaat tgatcttgaa gatccaaacg aaagctttct caaagaggca agcaaggaca
     4141 accagcttat aaacaacctt aaaatgaaat acattgaagt tgtctcaaaa cttgatgaga
     4201 gtgagaagta ttacggtgag aaatatccta aagttctctc gttaagcaca gag
//
LOCUS       DAOOYB010000248         4237 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_24615_length_4237_cov_8.892396, whole genome shotgun sequence.
ACCESSION   DAOOYB010000248 DAOOYB010000000
VERSION     DAOOYB010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4237
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24615_length_4237_cov_8.892396"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(304..906)
                     /locus_tag="PL056_11520"
     CDS             complement(304..906)
                     /locus_tag="PL056_11520"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HPM47689.1"
                     /translation="MKKFIFAVSVLFLAVSCDKGADNVYALEETGGQPVVKGSENGLM
                     WYIDDPDNANTKLNMMTYEKAVKNCSDLEWAGFTDWRLPTIDELRTLVTGFKDVETGG
                     RCKVSLKCLSMACLTQGQKSSADYPCSNYEEEALLQGPGPEGCYFDDVWRTYCGKHWS
                     SSKVAGVVDEMVFILDFSDPAISAVVSNVSNNMGFARCVR"
     gene            complement(906..1679)
                     /locus_tag="PL056_11525"
     CDS             complement(906..1679)
                     /locus_tag="PL056_11525"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HPM47690.1"
                     /translation="MKFFIHKKVEDIIVHNMQIPEIIGNIVDGIEVVITEPFFFRNDC
                     SEKIRKISDFCMNMDVSVHLPFYDLNLGSVDRNISEYSFRSMVQGIDIASGVGAEIAV
                     AHLGYNVLSSKSSTAKWFERFAVMKGELEKYAADKGVTVVWENTYEKEMAVFDEMIKV
                     HPGTKFCLDIGHCNCFAGFSAIEFLERYKESVVHIHLHDNDGIEDSHLAPGKGNIDFL
                     PIMEKIMAYPVRNAVFELDFDKFTDSVDHISEIFLKKRK"
     gene            complement(1683..2789)
                     /locus_tag="PL056_11530"
     CDS             complement(1683..2789)
                     /locus_tag="PL056_11530"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HPM47691.1"
                     /translation="MISKVKINRFIFDKKGWIPILFAAVFFFCVLFAPTITTILIASF
                     FAAYAIDPLVIFMSQKLKLPRSFASGFVIFFIVLTILLLLLIVLPGIVRQLYGVFANF
                     DSIVAALWDWMNNFASGFGIDLDEHFKKEDLVGRLTAFSEPVLKSATNTVGVVFSKTF
                     SLLSLLFSFMIFIVITFFTSSRYPKIRASIFELFPPAKQEYIKEWLCKFDRVLSGFIR
                     GQLTVCFVLGCLYAGAFTVAGIENAGSLGAMIGMLCIVPYVGLFAGITVTVLLALVSG
                     GVIALFKVVTVFLIIQSFDTVMITPNIMGRGVGMSPVFVIIAIFAGAEIGGFLGVLIA
                     VPAFAILKLLGDDILKRYKTSTFYNEKDNEGEPV"
     gene            complement(2786..3544)
                     /locus_tag="PL056_11535"
     CDS             complement(2786..3544)
                     /locus_tag="PL056_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47692.1"
                     /translation="MKIDEGIINITGKIVLITATGTPSINISNINTARIDAEKMARKN
                     LKFRLEKAFSKIVLEKGVDLGKHLASKKETDFFTGLIKDDDPAEIVSTRYFSDGSVEI
                     EYSVPVGKFLEQIKLKTMPDICRDEKMQTVFPEEMEGVADSRKDVLIIEIKSGKLDPA
                     LYLTLENEKGELIYSSCLSGTVPSMIVRKNPSRFLEILNSKESFLTVPALKVKSGSTV
                     VIGSTDAEKIANEMKRSSLKEGKVIFLIKEKEGK"
     gene            complement(3628..>4237)
                     /locus_tag="PL056_11540"
     CDS             complement(3628..>4237)
                     /locus_tag="PL056_11540"
                     /inference="COORDINATES: protein motif:HMM:NF019924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HPM47693.1"
                     /translation="VKVWFIENRYFQRDGLYGNITGDYPDNSERFVFFSRAVLELANV
                     ISRPDIIHCNDWQTALIPLYMKEVYQKNGQMKDVKTVFFIHDVRFQGRFWLYDLYILN
                     LGWEVFSPEKLEFYNDINFLKGGIVYSDALFAVNADYLTRICSDSEGCGLDGILRKYS
                     TKLFPVCESCKTFSCEDWKGERLGKRMLELYMQVYSSGVTAV"
BASE COUNT         1294 a          952 c          754 g         1237 t
ORIGIN      
        1 gtttgacccg gactgataaa actttttgag aaagggaaaa tccattgcag ttccaatctt
       61 gacagaagca gtgtcagagc ataaatgcaa accttaaatt caaatgtcca ggagcagggc
      121 gccgattgcc accttgtata acttaatata atgaggaaaa tccgtttgtc aagagaaaaa
      181 atgaaaaaat cttaatgttt tcaacttgtt gaagaacttt tgcttcatct tgtattcttt
      241 tttaaacacc tgttttttat acataccctc tctccatcaa taaacggcag tggctgattt
      301 tttttatctt acacagcggg cgaaacccat attgtttgaa acatttgaaa caactgcaga
      361 tattgcggga tctgaaaaat caaggataaa caccatttca tccacaacac cggcaacttt
      421 tgaagaagac cagtgttttc cgcagtatgt tctccagaca tcatcaaaat aacacccttc
      481 cggaccgggt ccctgaagaa gcgcctcctc ttcataattt gaacacggat aatcggcact
      541 gcttttctgg ccctgagtaa gacacgccat tgaaagacat tttaacgaaa ctttgcatct
      601 gcctccggtc tcgacatctt taaatccggt caccagagtt ctcaattcat caattgtcgg
      661 cagccgccag tccgtaaaac cggcccattc gagatcactg cagtttttta cagctttttc
      721 atatgtcatc atgttgagct tggtgtttgc attgtcagga tcatcaatat accacataag
      781 tccgttttcg gaacctttaa caaccggctg accgcctgtc tcttcaagtg catagacatt
      841 gtcagctcct ttgtcacagg aaactgccag gaacagaact gaaacagcaa aaataaattt
      901 cttcattatt tcctcttttt taaaaatatt tctgatatgt gatcaacaga atccgtgaac
      961 ttgtcaaaat cgagttcaaa cacagcatta cggacaggat atgccatgat cttttccatt
     1021 atgggcaaaa aatcaatgtt cccttttcca ggcgcaagat gagaatcctc aataccgtca
     1081 ttatcgtgaa gatgaatgtg cacaacgctc tctttatacc gttcaaggaa ttcaattgca
     1141 gaaaatccgg caaaacagtt gcagtgaccg atatccagac agaattttgt gccgggatgg
     1201 accttgatca tttcgtcaaa aacagccatc tccttttcat aagtgttttc ccagacgact
     1261 gtcacccctt tgtctgccgc atatttttca agctcacctt tcattaccgc aaacctttca
     1321 aaccattttg cagttgaact ctttgacgaa agaacattat atccaagatg ggcgacagct
     1381 atttctgcac ccacacctga agcaatgtct atcccctgaa ccattgatct gaaactgtat
     1441 tctgaaatat ttctgtcaac tgaaccaaga ttcagatcat agaacggcag atgtaccgaa
     1501 acatccatgt tcatgcagaa atctgatatt tttcttattt tctctgaaca atcgttccgg
     1561 aaaaaaaacg gctcagttat gacgacctct atcccgtcaa caatatttcc aattatttca
     1621 ggaatttgca tgttgtgaac aataatgtct tcaacttttt tatggataaa gaatttcatg
     1681 gatcagaccg gttcaccttc gttgtctttt tcgttataga atgtggaggt tttgtatctt
     1741 ttcaatatgt catcacccag aagcttcaat atcgcaaaag caggaactgc tataaggaca
     1801 ccgaggaatc cgccgatctc agcaccggca aagatcgcaa ttatgacaaa aacaggactc
     1861 atcccgacgc ctcttcccat aatgttcgga gttatcatta ctgtgtcaaa cgactggata
     1921 ataaggaaaa cggttacaac tttaaacagc gcgataacac ctcctgaaac aagagcgagc
     1981 agaaccgtca cagttatccc tgcgaaaagt ccgacataag gaacaatgca gagcattcct
     2041 atcatcgctc cgagacttcc ggcattttca atacctgcaa cggtgaatgc cccggcataa
     2101 agacatccga gaacaaaaca tactgtgagc tgacctctta tgaaaccgga aaggactctg
     2161 tcaaatttgc agagccactc ttttatgtat tcctgttttg caggtggaaa aagctcaaat
     2221 attgatgctc ttatcttcgg atatcgactg cttgtgaaaa aagtaatgac tatgaatatc
     2281 ataaaactga aaaggagtga aagaagactg aaggttttgc tgaaaacgac acctactgta
     2341 tttgttgcag acttcagaac tggctcggag aacgctgtaa gacggccgac aagatcttcc
     2401 tttttaaaat gttcatcaag gtctattccg aaacctgagg cgaaattgtt catccagtcc
     2461 caaagagctg ccactatcga atcaaaattg gcaaatacac cgtaaagctg tctgactatt
     2521 cccggaagca caatcagcag aagaagaaga attgtgagaa ctatgaaaaa gatgacaaat
     2581 ccgcttgcaa acgaacgggg aagtttaagt ttctgcgaca tgaaaattac aagagggtct
     2641 atggcgtatg cagcaaaaaa agatgcgatc agaattgttg taattgtcgg agcgaacagc
     2701 acgcagaaaa aaaatacagc ggcaaaaagg atcgggatcc accctttttt gtcgaaaatg
     2761 aacctgttta ttttaacttt agatatcatt ttccctcctt ttctttgatg agaaatatca
     2821 ctttcccttc ttttaaagag ctccttttca tttcatttgc tatcttttca gcatcagtgc
     2881 tgcctatgac aaccgttgac ccgcttttta ccttcaatgc aggaactgtc agaaaactct
     2941 cttttgaatt gagtatctca agaaaacgtg acgggttttt tctgacgatc attgaaggaa
     3001 ccgttcctga aagacaggag ctgtatatta attcaccctt ctcgttctca agtgtcagat
     3061 atagagcagg atcgagttta ccgcttttta tctcaattat aagtacatct ttgcgtgaat
     3121 ctgccacacc ttccatttct tcaggaaaaa cagtctgcat tttttcatct ctgcatatat
     3181 ccggcatcgt tttcaacttt atctgttcga gaaattttcc gaccggaaca ctgtattcta
     3241 tttcgaccga accatcagaa aaatacctgg tggaaactat ctctgcagga tcatcatctt
     3301 ttataaggcc tgtaaaaaaa tccgtttcct tctttgatgc aagatgttta ccaagatcca
     3361 ctcccttttc aaggacaatt tttgagaatg ccttttccag acggaacttt agattttttc
     3421 ttgccatttt ttccgcatct atccttgccg tgttaatgtt tgagatattt atggacggag
     3481 ttcccgttgc agttatcaaa acgatcttcc ctgtaatatt aatgatgcct tcatcaattt
     3541 tcataacaaa gtccgtgtca tcagaaactg gaaaaacata ctgaaaagaa aggagaaaaa
     3601 ggattaataa caggtttttc atcgcattta aactgccgtc acgccggaac tgtacacttg
     3661 catatacagt tcaagcattc tcttacccag tctttcacct ttccaatcct cacatgaaaa
     3721 agttttgcaa ctttcacaaa caggaaaaag cttagtgctg tactttctta aaataccgtc
     3781 aagaccgcaa ccttcggaat cactgcatat tctggtaaga taatctgcgt tgactgcaaa
     3841 aagcgcatcg ctgtaaacta tgccgccttt cagaaaattg atatcgttat agaactcaag
     3901 cttttcaggt gaaaaaacct cccacccgag atttagaata tagagatcgt aaagccagaa
     3961 ccttccctga aacctgacat cgtgaataaa aaacactgtt ttcacatctt tcatctggcc
     4021 gttcttctga tacacttcct tcatataaag agggatcagg gccgtctgcc agtcattgca
     4081 gtgtattata tcaggtcggg atattacatt ggcaagctca agtaccgccc ttgaaaagaa
     4141 aacgaacctt tcggagttgt caggataatc tccggtaatg tttccgtata atccatccct
     4201 ttgaaaatac ctgttttcaa tgaaccatac tttgaca
//
LOCUS       DAOOYB010000249         4235 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_24632_length_4235_cov_13.544976, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000249 DAOOYB010000000
VERSION     DAOOYB010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4235
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24632_length_4235_cov_13.544976"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..779)
                     /locus_tag="PL056_11545"
     CDS             complement(51..779)
                     /locus_tag="PL056_11545"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HPM47694.1"
                     /translation="MNRRSSDRISTEAIVRIIHKNEVYYSLSADISFGGINIICRLNF
                     SKEDILDIEFSIPQSNLSRPVKCKVKVLRSSIYEGNHHLHCSFVEISDMDTARFEEAL
                     NLLIIEAWFVADEEKPVKSSESRSDTRAHHRVPIKTWITNKDVDDHIHLPAENLSIGG
                     MYVITPAKHEPGSVLEIAFDLPGSGQTMEAVVTVVNIRPVGAMWGTGLKFIGLSEEDR
                     ELLEKVIHSDITARWFFSEQEEKE"
     gene            924..1772
                     /locus_tag="PL056_11550"
     CDS             924..1772
                     /locus_tag="PL056_11550"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HPM47695.1"
                     /translation="MFEKIRKTAEFIKEKVISGGFEQPNLLITLGSGLGYFAENTEKL
                     LVLPYSDIPGFPSSTVQGHAGRLILARDKKTGKNFWIMQGRVHYYEGYSMNDVVFPLR
                     VLIVLGIKKFIVTNAAGGVNRNFEAGDLMIIRDHINMMGSNPLIGRNIEEFGTRFPDM
                     TFAYSRKLGILMQKSAIENGITMKEGVYLALSGPTFETPAEVRMVSALGADAVGMSTV
                     PEVIAAKHAGLEVAGVSFISNKAAGLSSGPLTHEEVSENAALVEKRFGALMERFISLV
                     LEEKDG"
     gene            1765..2169
                     /gene="cdd"
                     /locus_tag="PL056_11555"
     CDS             1765..2169
                     /gene="cdd"
                     /locus_tag="PL056_11555"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF004064.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HPM47696.1"
                     /translation="MDNSTLQSAISQAADIRKNAYAPFSKFKVGAVVVAENGEIFQGV
                     NVENSSFGATVCAERIALFNAVTHGRKDIEAVVIVSVLNGKAVFPCGMCRQVLADFNP
                     EMRVILVNAETLKIEKDVLLSEIFPETFKFSN"
     gene            2183..3166
                     /gene="galE"
                     /locus_tag="PL056_11560"
     CDS             2183..3166
                     /gene="galE"
                     /locus_tag="PL056_11560"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HPM47697.1"
                     /translation="MKILVTGGAGYIGSHVVKLLGERGSDEIVVLDNLSTGRTDAVLF
                     GRLEVMDLSEFEKVDQFMKKEKFDAVIHFAAKIVVPESVANPLLYYRNNTVNTINLIN
                     SCVKYGVGMFIFSSTAAVYGEPEDGIVNENSLLSPISPYGTSKLMSEFVIKDTAFAHP
                     EFKYVILRYFNVAGADPDGLIGQNFPDATHLIKVACQTALGMRESLSVFGTDFNTPDG
                     TGVRDYIHVTDLASAHLAALDHLENGGNSDIYNCGYGKGTSVLEVIRSVRKVSDKDFP
                     VQNSPRRAGDPAKLIADNSKILKELNWVPKFQDLNLICRTAYHWEKKTLKR"
     gene            3240..4235
                     /locus_tag="PL056_11565"
     CDS             3240..4235
                     /locus_tag="PL056_11565"
                     /EC_number="1.8.4.11"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methionine sulfoxide reductase B/A
                     protein"
                     /protein_id="HPM47698.1"
                     /translation="MYFQKIIKNLLLIFLGFVIITALFAAEKEKKGENKMKKYNVNKT
                     DEQWRQELPPQKYKVLRESGTERPFTGIYNDLFDKGEYRCFACGALLFKSDTKFDHGC
                     GWPSFSESAVDGAIEYVPDHSHGMVRTEIKCANCGSHLGHVFDDGPKPKGTRYCVNSE
                     SLQFMPISEEKTMEKSENPVAIFAAGCFWGVEYKFSKQTGVISTDVGYTGGTVINPTY
                     QLVCTDATGHAEAVKVEYDQKLTDYESLVRFFFEIHDPTQVNRQGPDTGTQYRTAIFY
                     TTEEQKNIAEKVKKELQSKFDKPIATTIVPASKFYMAEEYHQEYVKKKGGESCGM"
BASE COUNT         1273 a          772 c          991 g         1199 t
ORIGIN      
        1 atagtgtccg gtgattatat agcagaattt tttctatttc aacttgattg ttattctttt
       61 tcctcttgtt cggaaaagaa ccatcttgcg gtgatgtctg aatggataac tttttcaaga
      121 agttcgcggt cttcctcgct gagcccgatg aatttgagtc ctgttcccca cattgctccg
      181 acaggtctga tatttacaac ggtgacaact gcttccatgg tctgtccgct tccgggaaga
      241 tcaaaagcga tctcaaggac acttccgggc tcatgcttag ccggagttat gacatacatt
      301 cctccgatag atagattttc cgccggaagg tgaatgtggt catcgacatc tttatttgtt
      361 atccatgttt tgatcggaac acggtgatgg gcacgggtat ctgatctcga ttctgatgat
      421 tttaccggtt tttcttcatc agcgacaaac catgcttcaa tgattagaag atttagcgcc
      481 tcttcaaatc ttgctgtgtc catgtcagaa atctcgacaa atgagcagtg aagatggtga
      541 ttcccttcgt atattgagga gcggagaact ttgactttgc attttacagg tctgctcaag
      601 tttgactgag gaattgaaaa ctcaatgtcg aggatgtcct cttttgaaaa attcagccgg
      661 cagatgatat taatgccgcc gaaacttatg tctgcggaaa gactgtaata aacttcattt
      721 ttgtggatga tccttacaat tgcttcagtt gaaattctgt cgcttgatct gcggttcatt
      781 tgttttttcc gtgtgattat caaaaatctg tctgaaatta tatactattt gaccgatttg
      841 tctaaaataa ttgacaaaaa ttgatatatt gaccatttgg tgatagattt ttaaccgatt
      901 cattgataaa ttcgaggttg aaaatgtttg aaaagatcag aaaaacagct gaattcatta
      961 aagagaaggt catatcaggt ggatttgaac aaccgaatct tctcataact ctcggaagcg
     1021 gactcggata ttttgctgaa aacactgaaa aactccttgt tcttccttat tcagatattc
     1081 ccggctttcc aagttcgaca gttcagggtc atgcaggacg gctgattctt gcccgtgaca
     1141 aaaagacagg aaaaaatttc tggatcatgc agggaagagt tcattactac gaaggatatt
     1201 cgatgaacga tgttgttttt cctctgaggg ttctcatagt tctaggaata aaaaagttca
     1261 tcgtcacaaa tgcggcaggg ggggtgaaca ggaactttga agcaggcgac ctgatgataa
     1321 tccgcgacca tataaacatg atgggaagca atcccctgat cggaaggaat attgaagagt
     1381 tcggcacaag atttcccgat atgactttcg catacagcag aaaacttgga atcctgatgc
     1441 agaaatctgc gatagagaac gggataacga tgaaggaagg tgtatatctt gctttgagcg
     1501 gcccgacttt tgaaactcct gcggaagtga gaatggtgag tgcactcggt gctgatgctg
     1561 tcggaatgtc aactgttccg gaagtgatcg cggcaaaaca cgcaggcctt gaagtcgcgg
     1621 gtgtgagttt catttcaaat aaggcggcag gactttcaag cggtcctctg acacacgagg
     1681 aggtcagcga gaacgcggcg ttagttgaaa aaaggttcgg agctttgatg gaaagattca
     1741 tatcacttgt tttagaggaa aaagatggat aacagcacac ttcaatctgc aatttctcag
     1801 gcggcagata tcagaaaaaa cgcttatgca ccattttcaa aattcaaggt tggcgcagtt
     1861 gttgttgcag aaaatggcga aatatttcaa ggtgttaatg ttgaaaactc ttcttttgga
     1921 gcaacggtgt gtgctgaaag gatcgccctt ttcaatgctg taactcacgg cagaaaagat
     1981 attgaagcgg ttgtgatcgt ttcagttctt aacggcaaag cggttttccc gtgcggaatg
     2041 tgcagacagg tgcttgcaga tttcaatccg gaaatgagag ttatccttgt taatgctgaa
     2101 acattgaaga ttgaaaaaga tgttttactt tcagaaatat ttccggaaac attcaaattt
     2161 tctaattaat aaggtggttt aaatgaaaat acttgtaact ggcggagcag gttatatcgg
     2221 cagtcatgtt gtgaagctcc ttggcgaaag gggaagtgac gagatagtcg ttctagacaa
     2281 tctttcaaca ggtcgaactg atgcggttct ttttggacgg cttgaagtga tggatctatc
     2341 cgaatttgaa aaggttgatc agtttatgaa aaaagaaaag tttgatgcgg tgatccattt
     2401 tgcggcaaag atcgttgttc ctgaatctgt tgcaaaccct cttctttatt acagaaataa
     2461 cacagtaaac accataaatt taataaattc gtgtgttaaa tacggggtgg ggatgttcat
     2521 attttcctca actgcggcgg tctatggtga gccggaagac ggaattgtca atgaaaatag
     2581 cttgttatct ccgataagtc catatggcac gagcaagctg atgagcgaat tcgtaattaa
     2641 agataccgct tttgcccatc cggaatttaa atatgtgatc ctgcgttatt tcaatgttgc
     2701 aggggctgat cctgatgggt tgatagggca gaactttccc gatgccactc accttatcaa
     2761 agttgcctgt cagacagcac ttggaatgag ggaatcttta tcagttttcg gaaccgattt
     2821 caatactccc gacggaacag gggtgaggga ttatattcat gtcacagacc ttgcgagtgc
     2881 ccatcttgca gcattggacc atcttgaaaa tggcggaaat tcagatattt ataactgcgg
     2941 atatggaaaa ggcacatcag ttcttgaagt tataagatca gtcagaaaag taagtgataa
     3001 agattttcct gtgcagaact caccaagaag ggcgggagat ccggcgaaac tcattgcaga
     3061 caattcaaaa atacttaaag aactcaactg ggtgccgaaa tttcaggatc ttaatctcat
     3121 ttgcaggaca gcatatcact gggaaaagaa aaccctaaag cggtaagact tttcccgcta
     3181 ccaattttta attcggtgat tatactctta attagtttgt ttgaaaaatg gagggtgtga
     3241 tgtattttca aaagatcata aagaatttac tgttaatttt tctgggtttt gtgataatta
     3301 cagcactttt tgctgctgaa aaggagaaaa agggggagaa caagatgaaa aaatataatg
     3361 tgaataaaac cgatgaacag tggaggcagg aacttccgcc ccagaaatac aaggttttga
     3421 gagagtccgg cacagaaagg cctttcacgg ggatttacaa cgatcttttt gataaagggg
     3481 aatatcgctg ttttgcctgc ggagcactcc tttttaagtc tgataccaaa tttgatcacg
     3541 gttgcggctg gccttcattt tcagaatcgg cagtggatgg tgcgatcgaa tatgttcccg
     3601 atcattctca tggaatggtg agaacggaaa ttaaatgtgc aaactgcgga agtcatctcg
     3661 gacatgtatt tgatgacggg cctaaaccga aagggacaag atattgcgta aattcggaat
     3721 ctttacagtt catgccaatt agtgaggaga aaactatgga aaaaagtgaa aatccggtcg
     3781 caattttcgc ggcgggctgt ttctggggag tggagtacaa attcagcaaa caaaccgggg
     3841 taatttcaac agatgtcgga tataccggag gaacagtgat aaatccgaca tatcagcttg
     3901 tctgcaccga tgcgacaggt catgccgaag ctgtaaaagt tgaatacgat cagaaactta
     3961 cagattatga atcactcgtg agattctttt ttgagataca cgacccgaca caggtgaata
     4021 gacagggacc cgacacagga actcagtata gaaccgctat tttctataca actgaagagc
     4081 agaaaaatat cgctgaaaaa gttaaaaaag aacttcagtc aaagtttgat aaacccatcg
     4141 caacaactat tgttccggca tcaaaattct acatggccga agaatatcat caggaatatg
     4201 tcaaaaagaa aggcggcgaa agctgcggaa tgtga
//
LOCUS       DAOOYB010000250         4214 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_24821_length_4214_cov_7.083193, whole genome shotgun sequence.
ACCESSION   DAOOYB010000250 DAOOYB010000000
VERSION     DAOOYB010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4214
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24821_length_4214_cov_7.083193"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(154..1086)
                     /locus_tag="PL056_11570"
     CDS             complement(154..1086)
                     /locus_tag="PL056_11570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HPM47699.1"
                     /translation="MDNWFLIANPVSGGNRCKRELADIIRILNSHKVPFTKALTKKRG
                     HATHLAIRAILEGYRKIICAGGDGTANEIINGILTQKTVLSTEITLAVIPLGTGNDWG
                     LSAGFTKDREQMVKAIKKGDTIVQDVCRTSFTGTDGTVKERYFMNVAGTGYDAEVLFK
                     TERMKESGKRGKWLYSLNIFTSLFSFKPTLTTIDIDGSNVISEKALSINIGIGRYNGG
                     GLMQVPHAVIDGGELALTFIGDIGRLGIIRHSGKLKTGDIGKVQKVQLLKGRNISITS
                     DRPMLFEADGEALGTTPLTFEVVPKTLKVVSVNS"
     gene            complement(1094..2194)
                     /gene="rodA"
                     /locus_tag="PL056_11575"
     CDS             complement(1094..2194)
                     /gene="rodA"
                     /locus_tag="PL056_11575"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HPM47700.1"
                     /translation="MIPRKKIDLMMVIPIVTILVLGLFSLNSALSHNISKFHIQLIWV
                     VLSIGVFMFFSYSDIRVFERSAIPLFVANIILLFLVLIIGKKIGGSQRWLDLGFMNFQ
                     VSELTKVSIILFIAYRLNMKPTLEDGYNLFDILPEFFATLVPVYLIYKEPDLGTALLV
                     IMTAMIMFLSTKINRKWLTGLIIFIIISSPFIWQYGLKKYQQDRIIALVSFVWSDNDD
                     LSLTTQYHTNQSVIAVGSGQIYGKGYKKGTQNMLRFIPEHHTDFIFSVFAEEFGFTGV
                     SFLLMLYLLLLVKILNLIGSVKDKFSSLILVGGVAHIWLQVLINIGMVTGIMPVVGIP
                     LPWFSYGGSSLIFNAILFGMIHNISINRRYSL"
     gene            complement(2191..4071)
                     /gene="mrdA"
                     /locus_tag="PL056_11580"
     CDS             complement(2191..4071)
                     /gene="mrdA"
                     /locus_tag="PL056_11580"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HPM47701.1"
                     /translation="MRILKQSEEVKSIYERTRLLLYVILFSFIILIGRVFYLQVIKGD
                     ELIEKSRDNFILSEKIFPARGEIFSLDGKVLATTEPSFRISVVPVFFLKPERKEEQID
                     RISTVLELSKNEKQRFSNQIMNCYGRCRYIPMLIKDEITKDKILSYSGYLTNFPGIII
                     SSTYKRVYPFREKTSHITGYVSKINQNEAKVLTDYDLDDFTGKTGLEKSYEEYLHGRY
                     GETFHIIDHMGRKIEVPENIDSSIPPAKYAVKGNSIKTTILSYLQETAASALGEMSGA
                     VVVMEISSGRILGMYSNPSFDSNLLSRKRIPEKIWREYSQSILNPLVNKVIRQTYFPG
                     STFKPIPAIAGLHYRKITPASTYLCEGCLMFGTDTKCCWNKGGHGYVNLYRSLKESCD
                     IYYYKLSEEIGLEGLTHFAALFGAGQVTGIDLPGEERGVLPERTWFNLNYPGMRIGKG
                     VIMNLSIGQGDIRMTPLQIAVLYAALANYGVIIKPRIVESIITPEGVVTPVENEIIRV
                     LDIDKKHFESINKALWSVTNEPGGTAYFHSDRTIKDAAGKTGTSQVISNIDRKNIDYT
                     DDERRHMTQDDALFAAFYPYRDPEIVAVAVIEGGGHGGSVAAPVVYKMLKSYHFGRSI
                     IE"
BASE COUNT         1106 a          925 c          902 g         1281 t
ORIGIN      
        1 gatatgcaga tgctcgcaac cgaaacagtc ctcaaacaag ccgaaatgat agccgaagag
       61 atagcgaaat caaaataacc gaacatcaaa gattgggcga aaacggtgtt tcgtccctac
      121 ggaaacgacg gacaagtccg cccctacgaa aattcacgaa ttgacgctta caactttcag
      181 cgttttcggt acaacttcaa aggtgagggg ggtggtgccg agtgcttctc cgtctgcttc
      241 aaaaagcata ggtctgtcgg atgttattga tatgtttctg cctttaagta attgaacttt
      301 ctgaactttt ccgatgtcgc ctgttttgag cttccctgag tgtctgatta ttccaagccg
      361 tccgatgtca cctatgaatg tgagtgcaag ctctcctccg tcaataactg catggggaac
      421 ctgcatcagc cctccgccgt tatatcttcc gattccgatg ttgatggaaa gagctttttc
      481 gctgattaca ttcgatccgt caatatcaat agttgtcaat gtgggcttga atgaaaagag
      541 tgatgtgaaa atgttcaatg aatagagcca tttgccgcgt ttgccagatt ctttcatcct
      601 ttcagtttta aatagcactt ctgcatcata accggttcct gcaacattca tgaaatacct
      661 ttctttgaca gttccatcag tccctgtaaa tgaggttctg catacatcct gaactatagt
      721 gtcacctttt tttatcgctt ttaccatctg ttcacggtct tttgtgaatc ctgcggaaag
      781 accccagtcg ttgcctgtcc ccagaggaat gactgcgaga gttatctctg tggataatac
      841 tgttttctga gtcagtattc cgttgatgat ctcatttgcc gttccgtctc ctcctgcgca
      901 tattattttt ctgtatcctt caagtatcgc tctgattgca agatgtgtcg catgcccccg
      961 tttttttgtc agagctttgg tgaaaggaac tttgtggctg ttgagaatcc tgattatgtc
     1021 ggcaagttct cttttgcacc tgtttccgcc tgagacggga tttgctatca gaaaccagtt
     1081 gtccattcag ctcttataat gaatatcttc tgtttatgct tatattgtgg atcattccga
     1141 aaaggattgc gttgaaaatg agagagcttc ctccgtatga aaaccatggc agaggaattc
     1201 ctacgacagg cattattcct gtcaccatcc cgatatttat taaaacctga agccaaatgt
     1261 gagcaacccc tccgacaagg atcagtgaag agaatttgtc ctttaccgat ccgataagat
     1321 tgagtatttt cacaagaagc agcagataca gcatcagcag aaaagagacc cctgtaaatc
     1381 cgaattcctc agcaaagaca gagaagatga aatcggtgtg gtgttcaggg atgaaacgga
     1441 gcatgttctg agttcccttc ttgtaacctt tgccgtagat ctgtccgctt ccgactgcaa
     1501 taactgactg atttgtgtga tactgtgtcg tcaagctcag atcatcgttg tcagaccaga
     1561 caaagctcac cagtgcgatg atcctgtcct gctgatattt cttcagtccg tactgccaga
     1621 tgaaagggga ggatatgata atgaaaatta tgagtccggt cagccatttt ctgttgatct
     1681 tcgtggaaag gaacatgatc atggctgtca ttatgacaag gagcgctgtt ccaagatcag
     1741 gttccttgta aatgagatat acaggcacaa gagtggcaaa gaattcagga agaatgtcaa
     1801 aaaggttgta cccgtcttca agtgtcggtt tcatgttaag ccggtaggca atgaaaagaa
     1861 ttatcgaaac ttttgtaagt tcgctcacct gaaagttcat aaaccccaga tcgagccatc
     1921 gctgcgaccc gccgatcttt tttccgatta tcagcacaag aaagaggagg atgatgtttg
     1981 caacgaaaag gggaatggcg gatctttcaa aaacacggat gtccgaatat gaaaaaaaca
     2041 tgaaaacacc gatcgagagc acgacccaga taagctgaat gtgaaatttt gagatgttgt
     2101 gacttaaagc actgttaagg gagaaaagtc cgagaacgag tattgtcact atagggatca
     2161 ccatcatcag atctatcttt tttctgggta tcattcgata atgctcctgc cgaaatgata
     2221 tgatttcaac attttataga ccaccggagc agccacactt ccgccgtgac cgcctccttc
     2281 aatgacagca actgcaacta tttcaggatc cctgtatgga taaaatgcgg caaaaagggc
     2341 atcatcctgt gtcatgtgcc ttctttcatc atcggtatag tcaatgttct ttctatcaat
     2401 attggagatc acctgactcg tcccggtctt tccggcggca tctttaattg ttctgtcaga
     2461 atggaaataa gcagttcctc ccggttcatt ggtaacactc cagagagctt tgtttatgga
     2521 ttcaaaatgc tttttgtcaa tgtcgagcac tcttattatc tcgttttcaa cgggagtcac
     2581 tacaccttcc ggagtgatta tgctttccac gattctcggc ttgatgatca ccccgtaatt
     2641 tgcaagcgcg gcgtatagaa cagctatctg gagcggagtc attcttatat cgccctgtcc
     2701 tattgaaaga ttcattatca ccccttttcc tatccgcatt ccgggatagt tgagattgaa
     2761 ccatgtcctt tccggaagaa cgcctctctc ttcgccggga agatcgatcc ctgtcacctg
     2821 accggcaccg aaaagtgcag caaaatgggt caacccttca agcccgatct cttcagaaag
     2881 tttatagtaa tagatgtcac agctttcctt aagcgatctg taaaggttga cataaccgtg
     2941 tccacccttg ttccagcagc atttggtgtc tgttccgaac ataaggcacc cttcacacag
     3001 atatgtcgat gccggagtga tctttctgta gtgaagtcct gcgatcgcag ggatgggctt
     3061 aaatgttgag ccgggaaaat atgtctggcg gataaccttg ttgacaagag ggtttaaaat
     3121 gctctggctg tattcccgcc atattttttc agggatcctt tttcttgaga gaagattcga
     3181 atcaaatgaa gggttcgaat acattccgag tattcttccg ctcgatattt ccatgacgac
     3241 gacagccccg ctcatttcac ccagagcaga tgccgcagtt tcctgaagat agcttaaaat
     3301 cgttgttttt atggagttcc cttttacggc atattttgcg ggaggaatgg aactgtcaat
     3361 gttttcagga acttctatct ttctgcccat gtggtcgatt atgtgaaatg tctctccgta
     3421 tctgccgtgg agatattctt catagctttt ttccagtccg gtcttacctg tgaaatcatc
     3481 aaggtcataa tctgtgagaa cctttgcttc gttctgattt atcttggaaa catatcctgt
     3541 gatgtgtgat gttttttccc tgaaagggta cacccgttta tatgttgacg atattattat
     3601 ccctggaaaa tttgtcaagt atccggaata actaagaatt ttatctttcg tgatttcgtc
     3661 ttttatcagc atcggaatat accggcatct gccgtaacaa ttcattatct gattactgaa
     3721 ccgctgtttt tcattttttg aaagttctaa aacggtgctt attctgtcga tctgctcctc
     3781 ttttctttcg ggtttcagga agaatacagg aacaactgat attctgaacg atggctctgt
     3841 agttgcaagc actttgccgt ccagggagaa tatttcaccg cgggcgggga atattttttc
     3901 actcagaatg aagttgtcgc gggatttctc aattagttca tcacctttta tcacctgaag
     3961 ataaaatact ctgccaatga gaatgatgaa ggaaaaaaga attacataga gcagaagtct
     4021 tgtcctttca tatatgcttt ttacttcttc tgactgtttc aggatcctca tagccaggag
     4081 tcctttatga agagttggtc aatggacggt ttaaaaaggt tatacgcaag aacaaggatg
     4141 acagccgata cggcagtatt tgctgtcatc cttgttcttg cgtataacct ttttaaaccg
     4201 tccattgacc aact
//
LOCUS       DAOOYB010000251         4194 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_24986_length_4194_cov_12.689055, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000251 DAOOYB010000000
VERSION     DAOOYB010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4194
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24986_length_4194_cov_12.689055"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="PL056_11585"
     CDS             complement(<1..263)
                     /locus_tag="PL056_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47702.1"
                     /translation="MSGWSGKSRGGGTGYYLFNLFLKYGGIHLAYFILRFVALYFVFT
                     APSSVKNLYFYFRKVHGYGFLRSCFSIYLNFVELGESIIDRMAV"
     gene            complement(273..524)
                     /locus_tag="PL056_11590"
     CDS             complement(273..524)
                     /locus_tag="PL056_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016269461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HPM47703.1"
                     /translation="MKRAEIIEKIISFMSEEFEVEPEVIKPEAELMKTLDLDSLDLVD
                     LVVIIEKNFGFKVEAKDFVNIKTFGDFFEYVCQKLNITE"
     gene            complement(543..1766)
                     /locus_tag="PL056_11595"
     CDS             complement(543..1766)
                     /locus_tag="PL056_11595"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HPM47704.1"
                     /translation="MRRVVVTGMGIHSCIGYSIDEVRDSLFKGKSGIVFDPERKQLGF
                     RSALTGKVDMPDIKGKVSRRERVGMADEGIYAYAATDQAFLHAKIDMDFLEKNEVGIL
                     YGNDSSAKAVIDAADIMREKKDTMLLGSGAIFQGMNSTVTMNLATIFKLRGINFTISG
                     ACASGSHAIGLGYLMIRSGLQEIVLCGGAQEVNHHSMASFDGLSAFSTRESEPEKACR
                     PFDKDRDGLVPSGGAASLILESYESAVKRGVPILAEVVGYGFSSNGSHISVPNVDGPL
                     RSLNMAIKDSGIRINDIDYVNAHATATPVGDMNEAKAIDLAFGDHKPFVSSTKSMTGH
                     ECWMAGASEAVYSIIMMHNDFAAPNINFSEPGEEAKPLRIIPEAVSTKVETILSNSFG
                     FGGTNSTLILRKFVK"
     gene            complement(1782..2510)
                     /gene="fabG"
                     /locus_tag="PL056_11600"
     CDS             complement(1782..2510)
                     /gene="fabG"
                     /locus_tag="PL056_11600"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016274635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HPM47705.1"
                     /translation="MKIALVTGGSRGIGKAISLKLASDGLHVIVNYRSNDVEAVKTLE
                     IIKENGGSGELLKFDVANRSEVRTVIDGWKAANPEKFIEVLVNNAGIRKDNLLMSMDD
                     SEWDSVINTNLNSFYSVTSSVIKEMMYNKSGRIVNIASLSGIKGLPGQTNYSASKAGM
                     IGAAKALAQEVGRRGMTVNVVAPGFIHTEMIEGINESDFRKLIPLGRFGKPEEVANVV
                     SFLVSDSASYITGEVISVNGGLYT"
     gene            complement(2518..4053)
                     /locus_tag="PL056_11605"
     CDS             complement(2518..4053)
                     /locus_tag="PL056_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HPM47706.1"
                     /translation="MITIGARYISFDDFIDIVFKSEKIELSPESLSEVEENFNFLKSF
                     AKNRTIYGINTGFGPMARYKIADEDVEQLQLNLIRSHAAGTGKILPDIYARGAVLARL
                     NSLMLAKSGVHPDVVHLLAEFLNNNISPVIFEHGGVGASGDLVQLAHLALNLIGEGEV
                     YLNGKIVPASTAFERCGLAPIKMHLREGLALINGTSVMTAIGLTNLYQSKRLFDWMIG
                     ASAIMMEVVECYDDHYSIETNSVKLHSSQNTVAVKLSKLLNGSSLVKSRKINMYDTEL
                     APGFIDEKVQEFYSIRCVPQVLGPVLETLDFSQKILIDEINSVNDNPVIDHTNQFVYH
                     GGNFHGDYVSLEMDKMKIAVTKLTMLSERQLNYLMNPKLNEKLPPFVNLGRPGFNYGM
                     QAVQFTAVSTTAENQTLCFPNCVHSIPNNNDNQDVVSMGTNSANIARTVINNAFEVVA
                     IELMALIQAVDCLECSSKMSVNTAEIYSSLRKIVPAFKEDSPKYIQISSMKEHIYNND
                     FTV"
BASE COUNT         1197 a          986 c          752 g         1259 t
ORIGIN      
        1 acagccattc tgtcgataat tgattccccg agttcaacaa agttcagata gatagaaaaa
       61 cagctcctca gaaatccgta tccatgaacc tttctgaaat aaaaataaag gttctttacc
      121 gatgacggag cggtaaaaac aaaatagagt gcgacaaaac ggagaatgaa ataggcaaga
      181 tgtatcccac cgtattttaa aaaaagattg aaaagataat atcctgttcc gccaccgcgg
      241 ctttttccag accagccgga catttaattg aattactcag taatattgag cttttgacag
      301 acatattcga agaaatcacc gaaagtctta atgtttacaa aatcttttgc ttcaacttta
      361 aaaccgaagt ttttctcaat gataacaaca aggtcaacaa gatcaagact gtcaagatca
      421 agtgtcttca tcaactcggc ttccggcttt atgacttccg gctcaacttc aaattcttca
      481 ctcataaaac taatgatctt ctcaatgatc tcggctcttt tcataacttg ctccaaaaaa
      541 aattatttaa cgaacttcct taaaatcaat gttgagtttg ttccgccgaa accgaaagaa
      601 ttgctgagaa tggtttcaac ttttgtagaa accgcttcgg gaattatcct caaaggtttc
      661 gcctcttcgc ccggttccga gaaatttata ttcggggcgg caaaatcgtt atgcatcatt
      721 attatcgaat atactgcttc gcttgctccc gccatccagc attcatgacc tgtcattgac
      781 tttgtgcttg aaacaaacgg cttgtgatcg ccgaatgcaa ggtctatcgc tttcgcttcg
      841 ttcatgtccc cgacaggagt tgccgtagcg tgtgcgttga cataatctat atcatttatt
      901 cttatccctg aatctttaat tgccatattc agagatctca aaggtccgtc cacattcgga
      961 actgaaatgt ggcttccgtt tgaagaaaat ccataaccta ccacttctgc aagtatcgga
     1021 actccccttt ttacagcact ttcgtaactt tcaagtatca aagatgctgc tccgccactt
     1081 ggaacgagtc cgtcgcgatc tttgtcaaat ggtctgcatg ctttttccgg ttctgattct
     1141 cttgtcgaaa acgcgctgag tccgtcaaaa ctcgccattg aatggtggtt cacttcctgt
     1201 gctccgccgc acagaactat ttcctgcaga cccgatctta tcatcagata tccaagtccg
     1261 atcgcatgag aaccacttgc acaggcaccg ctgatggtga agtttatacc acgaagttta
     1321 aatatcgttg caagattcat tgttacagtt gaattcattc cctgaaaaat agcaccactg
     1381 ccaagaagca tggtatcttt cttttctctc ataatatctg cggcatcaat tacagctttt
     1441 gcagaactgt cattgccgta aagtatcccc acttcatttt tttcaagaaa atccatatca
     1501 atttttgcat gtaaaaatgc ctgatcagtt gcggcataag cataaattcc ttcatcagcc
     1561 attccgaccc tttctcttct tgaaaccttc cctttaatgt caggcatatc aacttttcca
     1621 gtgagagcac ttctgaaacc gagttgcttt ctttcaggat caaaaacaat accgcttttt
     1681 cctttgaaaa gagaatccct gacctcatca atcgaataac cgatgcagga atggatcccc
     1741 atgcctgtaa caaccactct tctcatatat aaccccttga attatgtata caatccgcca
     1801 ttaaccgaaa taacttcacc cgtaatgtaa gaagcagaat ccgacacaag gaaagagacc
     1861 acatttgcaa cttcctcagg ttttccgaac cttccaagcg ggatcagttt cctgaaatca
     1921 gactcgttta tcccctctat catttctgtg tgaataaacc ccggagcaac gacatttacc
     1981 gtcattcccc ttcttccaac ttcctgagca agcgcttttg ccgcaccgat cattccggct
     2041 ttcgatgcag aatagttcgt ctgtcccgga agtcctttta ttcctgaaag acttgcaatg
     2101 ttcactatcc ggcccgattt gttgtacatc atctccttta ttactgagga agtaactgaa
     2161 tagaaagaat tgaggttagt gttgataact gaatcccact ctgaatcatc catagacata
     2221 agcagattgt cctttcttat tcctgcatta tttacaagaa cttcaatgaa tttttcagga
     2281 tttgccgctt tccagccgtc aatgaccgtt ctcacttcac tgcggtttgc gacatcaaat
     2341 ttcagcaact caccgcttcc gccgttttct ttaattattt caagcgtttt aacagcttcg
     2401 acatcattac tgcggtagtt tacgatcaca tggagaccgt cactggcaag tttaagcgaa
     2461 atcgcttttc ctattcctct tgatcctccg gtaacaagtg caattttcat atcatcctca
     2521 aactgtaaaa tcgttgttgt aaatatgttc cttcatgctg ctgatctgta tatatttcgg
     2581 agaatcctct ttaaatgcgg ggacgatctt ccttaaactg ctatatattt cagcggtgtt
     2641 aactgacatt tttgatgaac attcaagaca gtcaactgcc tgaattagtg ccataagttc
     2701 gatcgcaacg acctcgaagg cattattgat aacggttctt gcaatatttg ccgagttggt
     2761 tcccatgctt acaacatcct gattgtcatt gttgtttgga atggagtgaa cacagttggg
     2821 aaaacataaa gtctgatttt cagccgtggt tgaaactgct gtgaactgaa ccgcctgcat
     2881 cccgtaatta aatcccggcc tgccgagatt tacaaaagga ggaagctttt cgttaagttt
     2941 cgggttcatc agatagttaa gctgtctttc agaaagcatt gtcagttttg ttacagcgat
     3001 cttcatctta tccatttcaa gtgaaacata atctccgtgg aaattcccgc cgtgatagac
     3061 aaactgatta gtgtggtcaa tgaccggatt atcgttaact gaatttattt catctatcag
     3121 tattttctga gaaaaatcaa gtgtttccag caccggtcca agaacctgag gaacacatct
     3181 gatcgaatag aactcctgaa ccttttcatc aatgaaacca ggggcaagtt cggtatcata
     3241 catatttatc tttctgcttt tgactagtga gctgccattg agaagctttg aaagtttaac
     3301 ggcgacagta ttctgcgagg aatgaagctt tacactattc gtttctatcg aataatggtc
     3361 gtcatagcac tcaactactt ccatcatgat agcactggct ccgatcatcc agtcaaaaag
     3421 ccgctttgac tgataaagat ttgtaagtcc tatcgctgtc atgaccgatg ttccgttaat
     3481 gagcgcaagt ccttcccgaa ggtgcatttt tatcggagcg agtccgcacc tttcaaaagc
     3541 cgttgacgct ggaacgatct ttccattgag gtacacttcc ccttccccga taaggttgag
     3601 agcaaggtgt gcaagctgaa ccagatctcc gctcgccccc actccgccgt gttcaaaaat
     3661 gactggtgaa atattgttgt tcagaaactc tgcaagaaga tgcaccacat cgggatgaac
     3721 accgcttttg gcaagcatga gcgagttaag acgggcaaga acggcacccc ttgcataaat
     3781 atcgggaagt atctttcctg ttccggcagc atgacttctg atcagattca gttggagctg
     3841 ttcaacatct tcatcggcaa ttttgtatct tgccatcgga ccgaaacccg tattaattcc
     3901 gtaaattgtc ctgtttttag caaagctttt aagaaaattg aaattttcct caacctcaga
     3961 cagagattca ggagaaagct caatcttttc agatttaaaa actatatcaa taaagtcatc
     4021 aaaactgata tatcttgcac ctattgtgat catctttttt cccgtcccgc caaaagagtt
     4081 atcaaaattc tcaaaaaaca gccgaaagat tatagcgaaa caataatcag ggtcaaataa
     4141 tcgtatcaag ccatctaaaa tcttaccgaa gacaaatatg tcacgccatg taaa
//
LOCUS       DAOOYB010000252         4189 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_25044_length_4189_cov_6.392356, whole genome shotgun sequence.
ACCESSION   DAOOYB010000252 DAOOYB010000000
VERSION     DAOOYB010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4189
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25044_length_4189_cov_6.392356"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            384..1922
                     /locus_tag="PL056_11610"
     CDS             384..1922
                     /locus_tag="PL056_11610"
                     /inference="COORDINATES: protein motif:HMM:NF021637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HPM47707.1"
                     /translation="MAKEKNIPQTTGIVSYKAPTPKVEEEIYNLQDDSALIQYEMNMI
                     ELPFFTRDKNVGEAIIKKYVFSGNQYMEVIPSSDKSSGYKIPQEFDEKIFYAIMQLYR
                     KQGEKRIVTTMYELLKLAGYGYGKKEYDRVKQSLDRLKDTVYVFNGIIYDKKNNRIVK
                     DRVRKNILQSSGVREFSDLNKEELENFGREIPGKSVVVIEISDFIEENIAAKGFLYYD
                     AEKLLDVTNSTARKLYLLISKWQGWEKSDTIRRSCNFLASRIPLSWEKSNIPGTINVL
                     EKASEELKEKGMIGGYVLNRRKPLSESEIIFMFQGATETARSKAIMNANLDLSTETGH
                     EGIVIDAVRDEFLDDRQTSIFDTPGFRETEILLSLLPEEYRTETNRKTIERFLAKGSE
                     HIKSNIRYTLKNCNGNFEAYLNKALSGDWAASDREKAELEREKKHKEQIEAEKKTEDL
                     RKKATEIYKNLKDDGLNKMHREAEHSTYYRFSFEDRVNKGEIEKKEALREVALVLISG
                     RIDK"
     gene            1949..2458
                     /locus_tag="PL056_11615"
     CDS             1949..2458
                     /locus_tag="PL056_11615"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPM47708.1"
                     /translation="MFEELFREARKNYDQGEYVAAIAKLTQIIDSDSKNGRKKSASAY
                     QLRGLSYIKMNDNYEAAEKDFQTALELEPENPYILSDMGCLEHKKGNYREAVSFFDRA
                     IELNPDNYITYLNKVYTLERSQKYREALDILPVICNLRENKGLGEDPEITKMRLWFEK
                     QIELDGRIS"
     gene            complement(3261..3548)
                     /locus_tag="PL056_11620"
     CDS             complement(3261..3548)
                     /locus_tag="PL056_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47709.1"
                     /translation="MKFTKKQRARIDLVNEHLKKGDLITHVCDGGMLAEHYYARTEKS
                     GNIIGIPTAETKKFIGGFLSGVYIHPVNITHINRQSVESIPFLVSFRPKSI"
     gene            complement(3562..>4189)
                     /locus_tag="PL056_11625"
     CDS             complement(3562..>4189)
                     /locus_tag="PL056_11625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47710.1"
                     /translation="SDYYKICGDDNDTTGVKKRYRHFGKKFKAKQDRVSYNSSSNTYR
                     IYYKPNWDTYYFDFSPSGSPVNNCSSDAHCPATADFFSLSEGCENHYDWDPEEGYYKL
                     CKPVLTKHYTDTTADISHYNICSYEDMGKCLSYYQTCPAPSESVYEFYWYKKLPECGL
                     RRDFRYFPKDLKLSITDDFSRKVTVTNASAGTYTDHTTCPQEKKITAQ"
BASE COUNT         1460 a          736 c          901 g         1092 t
ORIGIN      
        1 gaaaccacgg taaattctat gatgaaacca cggtaaattc tatgatgaaa ccacggtaaa
       61 ttctatgatg aaaccacggt aaattctatg atgaaaccac gataatttct atgatctgca
      121 aacaaaaacg acgggtttta tgatgaaact acgatagttc ctatgatgaa accacggtaa
      181 aaagacatcg ttttgcaaaa aatatgataa aagcccgata aaaaacttga ttttatgatg
      241 aaaccacgat aaaaagaacc ggatgaaatt tgactgaaag gtgttttgat gcttgaaaga
      301 acaaatggcc tgagaaaaaa acacactgat aacagagtgt tacaaacatt ctatgatgaa
      361 accacggtaa tttggaggtt gtgatggcaa aagaaaaaaa tattccgcag accaccggga
      421 ttgtgtctta taaagccccg acccctaaag tcgaagaaga aatttacaac cttcaggatg
      481 attccgcact gatacagtat gaaatgaaca tgatagaact tcccttcttc acacgggata
      541 aaaatgttgg tgaagcaatc atcaaaaaat atgtattctc cgggaaccag tatatggaag
      601 ttattccttc atctgataaa tcttccggat acaagattcc ccaggagttt gacgagaaga
      661 ttttctacgc aatcatgcag ctctaccgta agcagggcga aaaaaggata gtcacaacca
      721 tgtacgaact tctgaaacta gcggggtatg gatacggaaa aaaggaatat gacagagtta
      781 aacagagcct tgaccgtctg aaagacactg tttatgtatt taacggaata atatacgaca
      841 agaagaacaa cagaattgtt aaagaccggg taagaaaaaa catactgcaa tcttccggag
      901 tcagagaatt ttcagacctg aataaagagg aacttgaaaa cttcggaaga gaaatacccg
      961 gaaaatcagt tgtcgtaatt gaaatctccg atttcattga ggaaaatata gccgcgaaag
     1021 ggttccttta ttacgatgct gaaaaactcc tcgatgtgac aaattcaacc gcaagaaaac
     1081 tttatcttct catttcaaaa tggcagggat gggaaaaaag cgacacaata agaagatcct
     1141 gtaactttct agcttccaga attccgcttt catgggaaaa atcaaatatc ccaggcacaa
     1201 taaatgtatt ggaaaaagct tctgaagaat taaaggaaaa aggcatgatc ggcggatatg
     1261 tgcttaacag gagaaaaccg ctatctgaat cagaaataat attcatgttt cagggagcta
     1321 ccgaaacagc aagatcaaag gcgataatga acgcaaactt agacctgagt actgaaacgg
     1381 gtcatgaagg tattgtaatc gatgctgtgc gggacgaatt tcttgatgac agacagacaa
     1441 gtatttttga tactcccgga ttcagagaaa ctgaaatact tttaagtctt ctcccggaag
     1501 aataccgcac ggaaacaaac agaaaaacaa ttgaacggtt tttagcaaag ggatctgaac
     1561 atataaagtc aaacataaga tatactctta aaaactgtaa cggtaatttt gaggcgtacc
     1621 ttaacaaagc actctccgga gactgggcgg caagtgaccg ggaaaaagct gaacttgaaa
     1681 gagaaaaaaa gcacaaagaa cagatcgagg cagaaaagaa aactgaagat ctccggaaaa
     1741 aagctacaga gatatacaaa aacctcaagg atgacggtct taataaaatg cacagagagg
     1801 cggagcattc tacatattac agattttcat ttgaagatag ggtgaacaag ggggagatcg
     1861 agaaaaaaga ggcgttaagg gaagtagcgc ttgttctcat ttcgggaagg atcgataaat
     1921 gaaaaaacaa caaataggag gattagaaat gtttgaagaa ttatttagag aagccagaaa
     1981 aaattatgat cagggtgaat atgtggccgc aattgcaaaa ttaactcaga taattgacag
     2041 tgacagtaaa aatggaagga aaaaatcggc atcggcttat cagttgaggg gattgagtta
     2101 tataaaaatg aatgataatt acgaagccgc tgaaaaggat tttcagactg cccttgaact
     2161 tgaaccggag aatccttaca tattgtcaga catgggatgt cttgaacata aaaaaggcaa
     2221 ttacagggag gcggtgtcat tctttgacag agcaattgaa ctgaacccgg acaactacat
     2281 tacatacctg aataaagtat acacccttga aagatcgcaa aaatacaggg aagctcttga
     2341 tattctgcct gtgatatgca atttaaggga aaataaaggg ctcggggaag atcctgaaat
     2401 aacaaaaatg cggctgtggt ttgaaaaaca gattgaattg gatggacgga taagctgaaa
     2461 aggaaaaaat tgaattattc cagacaggtg atatgtttaa aaaccgtttc aagcggtatt
     2521 cgtttacaca aagtttccga ttcaaatcca atcgaaatct gtcctgcgct cagcaggact
     2581 tcatcatctt aaatcatttg aaaaggttta acggcaattc aatgtcataa ctgtctgaaa
     2641 ttattggaac cggatttgtg ccgggaatcg agatccggac tgccggcagt tcatggccgc
     2701 aaaaatcagt taatggggat tagggaagaa aaagggatgg aaaggacttg ataattaaat
     2761 caagtccttt ccaaactgca ctagaaacaa atccagtaca gttcagcgtt gcctttcaaa
     2821 gagttcaatc cgagattaag aattggggga gattggttag atctcccctt cttctcaaac
     2881 tttccaagca ataaagcctg ataccgttgc aaccgacaaa agtacccaga aattcggatc
     2941 agccaaacta atgaccaaaa gaatcgtctg gaaaagtcca aggaagattt ccagtctgcg
     3001 agacatgaaa acctccaagt ctaagattga taaactcaaa gaacaaacaa cacactgata
     3061 atacttgact taatagaatt tgtccataaa atagtaattg cgggacatga aaattttgaa
     3121 taaaccccgg acttctgaaa aagaggttcg gggttttctt ttttgacatt gtggtcagtt
     3181 gcaataaaat aatgtttgtg aggcattgga ttttataagt cccctgacat cctgaaaagg
     3241 gtgtcggggg atttctgttt ttaaatactc ttagggcgga atgaaaccag aaacggtatt
     3301 gattcaactg actgtctgtt aatgtgagta atgtttacag ggtgtatgta gacccctgaa
     3361 aggaatccgc ctatgaactt ttttgtttct gcggtgggaa taccaataat gttcccggac
     3421 ttttctgttc tcgcatagta atgttcagca agcattccgc cgtcacagac atgagttatg
     3481 agatcacctt ttttaaggtg ttcattcacc agatctattc tggctctctg tttttttgtg
     3541 aatttcataa tacacccgtg tctattgtgc cgttattttc ttttcctgcg gacaggttgt
     3601 gtgatcggtg tatgtacccg ctgaagcatt ggtaacagta acttttctgc tgaaatcgtc
     3661 agttatgctg agcttgaggt ctttaggaaa ataacggaaa tcccttctta aaccgcattc
     3721 aggcagtttt ttataccagt agaactcata aacagattca gagggagcgg gacaggtctg
     3781 ataataggag agacacttgc ccatatcttc ataagaacag atattgtaat gtgaaatatc
     3841 agcggtggtg tcggtatagt gttttgtgag aacaggttta catagtttgt aatatccttc
     3901 ttccgggtcc cagtcataat gattttcaca accttcggaa agtgagaaaa agtccgcagt
     3961 cgccggacag tgtgcgtcac tgctgcaatt attaacggga gatcctgacg gtgaaaaatc
     4021 aaaataatag gtgtcccagt tgggtttgta atatattctg tatgtattgg aagagctgtt
     4081 atatgaaact ctatcctgtt ttgccttgaa tttcttcccg aaatgacggt accgtttctt
     4141 aactcccgtc gtatcgttgt catcaccgca tattttgtaa taatcgctg
//
LOCUS       DAOOYB010000253         4182 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_25112_length_4182_cov_12.573055, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000253 DAOOYB010000000
VERSION     DAOOYB010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4182
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25112_length_4182_cov_12.573055"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..1493
                     /locus_tag="PL056_11630"
     CDS             111..1493
                     /locus_tag="PL056_11630"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HPM47711.1"
                     /translation="MRTVIAALLLLFFFNILSEERTYTISMMGNEIGKNTEKWTESKD
                     FSGKCILTLESTSKMGITRGAYSVSMETRSVVKVTCKDFFPVSVESESSEMSSKMNSK
                     AVNVNGFFSGEIEKNGNREPVSFTVPQNATFFSMIFRKFSENDFLKGGSAKIISEESL
                     TLKNISYSAVKEPSGLLKVTVNYDGVPLSFFLDKDLVVRSDLQNGLISYKLDGYLKSP
                     QTLPEQSGNADILVNTSISNKGISVKNPRSATKMNFSVSGNNILEIPETCFQKQGDNG
                     TLTVDNNSKFCPALPVPQDTQGNIFEDSGNPKIAAAAQKVIKGSPDKNEMIKRMVNFV
                     FRHIKNKNYSHGNLSAGEVLENRSGDCTEHATLLSALLKSAGIPVKMAYGVVLDDKGG
                     FMFHNWNEVYGDNGWMSVDATFGAVKADAARILLIYGGSDSSSREQVSLAVLKFLGSL
                     EISVTGFANE"
     gene            1486..2307
                     /locus_tag="PL056_11635"
     CDS             1486..2307
                     /locus_tag="PL056_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47712.1"
                     /translation="MNKKWFKSAAVTGSGFEHLADETIGFSEISNEKLRGVDLLFVKN
                     KRDFFSKFRFNGSTVISDMAINSEDQEKFILMSPGDLFISRIGHDIVNIFSPFEHFEY
                     LEENMITDRVNQGIKFGRQINIIGHLVQNTKKWSESRYDFENFILTVQGLCRKSGIEI
                     KIQNPHKYRIDTIPDADIMNPVIDEIVSNLKMHGSREFELAVVSNTEMVFRNDFDCSF
                     NFERPKALLRCPFTKRTNSPGSGLGLFIFSLSSVRGGFDWDISVKENQFCLSLFF"
     gene            2256..3179
                     /locus_tag="PL056_11640"
     CDS             2256..3179
                     /locus_tag="PL056_11640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47713.1"
                     /translation="MGHISKRKSILFVIVFLTNGFLLGEGYFNPDNDPIWSDTSFGVR
                     ARELSHSIDTQNCGKGCGALLSHNSQYLFNPANAMLQQQFTLSLLYHYLDSMSASVSD
                     SKTSKVAGGVMYSRHAGLNHIKTNFAFPIYKIVFGGLNVNNYIGKFAPTQTRSTNSHS
                     FDLGISAVITKFLYVGFGALDVWTFSGKDIPRKLNWQFELNFNNMFFFNNGWQYHLEP
                     EKEKFPNKRSKLKDFDFYTGFEFRYSWFRAAIGFNNLSFNEDFTWDTTLKTFSIALYK
                     PGAGGIYGSFMYNEAEYMTFTINIVWEPPVQ"
     gene            complement(3180..4034)
                     /locus_tag="PL056_11645"
     CDS             complement(3180..4034)
                     /locus_tag="PL056_11645"
                     /inference="COORDINATES: protein motif:HMM:NF022932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPM47714.1"
                     /translation="MSDFETIVAKLIKLGFNRLEAEVYLTLTRESPLTGYRIAQILNK
                     AAANTYKALESLEKKGAVVAEETPTVKQYAAIPVSDYLDQLESGLRNDRKFLEESLRD
                     INYSLSGDKIYMLQTLDQVLEKCSKMLENAWHSVIVDAFPPMLAKIAPMLEKAAGNGV
                     NIIVNAYEPVEVKNCRVIVKSNAAKVLTLWSGDWMNIAVDGREYLITALEKDGTLQHA
                     IWSNSPYLSFFVFNGFRYEMLFSALSSKIPSEKKNEILNSAFADINIDIADLPGYKTM
                     LSRLGKKN"
BASE COUNT         1255 a          830 c          861 g         1236 t
ORIGIN      
        1 aataatattc attgatctat ttttcaaacc gggcggattc cgcccggttt ttttttgctt
       61 ttacaactca attatctcat aatattatga actttttgct aaggagcttc atgagaacag
      121 tgattgcggc attgttactt cttttctttt tcaacattct gtcagaggaa cggacttaca
      181 ccatttcgat gatgggaaat gagatcggaa aaaacacaga aaaatggaca gaaagcaaag
      241 atttctccgg gaaatgcatt cttacacttg agtcaacttc taaaatgggg ataaccagag
      301 gcgcatactc tgtcagcatg gaaacgagat cggtcgtgaa ggtgacctgc aaagactttt
      361 tccctgtttc ggttgaatct gaaagtagtg aaatgtcctc aaaaatgaat tcaaaagctg
      421 taaatgtgaa cggatttttc agtggcgaaa tagaaaagaa cggcaacagg gaacctgtat
      481 catttactgt tcctcagaat gcaacttttt tcagtatgat attcagaaaa ttctctgaaa
      541 atgatttttt aaaagggggc agtgcaaaga tcatttccga agaatctctc actttgaaga
      601 acatttcata ttccgctgta aaagaacctt caggacttct caaagtgacg gttaattatg
      661 atggggtgcc tctcagtttc tttcttgata aggatctcgt tgtgcggtca gaccttcaaa
      721 acgggctaat atcatataaa ctggatggat atctcaaatc accacaaact ttacctgaac
      781 aatcaggaaa tgccgacatc cttgtgaata catctatttc caacaagggg atctctgtca
      841 agaatccaag atctgcgacg aaaatgaact tctctgtttc ggggaataat attttggaaa
      901 tacctgaaac ctgttttcag aaacaaggag acaacggaac actcacagtt gacaacaatt
      961 caaagttctg cccggctctt ccagttccgc aggatacaca gggaaacatt tttgaagaca
     1021 gtggaaaccc gaagatagct gccgcagcac aaaaagtgat caaaggatcc cccgataaga
     1081 acgagatgat aaaacggatg gtcaattttg ttttcagaca tattaaaaac aagaattaca
     1141 gtcatggtaa tctttcagca ggcgaagttc ttgaaaacag atcgggagat tgtactgaac
     1201 acgcaactct tctttctgcg ttgctgaaat cggctggaat tcctgttaaa atggcctacg
     1261 gtgttgttct tgacgataaa ggcggattca tgttccacaa ctggaatgaa gtgtatggcg
     1321 ataatggatg gatgtccgtt gacgctactt tcggagcggt aaaggccgat gcggcgagaa
     1381 ttcttcttat ttacggagga agcgacagca gttccagaga gcaggtttca ctcgcggtat
     1441 taaaattcct cggttcgctt gaaatttcag tgacggggtt tgcaaatgaa taaaaaatgg
     1501 tttaaaagtg ccgctgttac agggagcgga tttgaacatc ttgctgatga aacgatcgga
     1561 ttttctgaga tctcgaatga aaaactgaga ggagtggatc ttctttttgt caaaaacaaa
     1621 cgggactttt tttcaaagtt cagattcaac ggatctactg taataagtga tatggcgatc
     1681 aattcagaag atcaggaaaa atttattctt atgtcacccg gagatctgtt cataagcagg
     1741 atcggtcatg atattgtcaa tattttcagc cctttcgaac attttgaata tcttgaagaa
     1801 aacatgataa ccgaccgtgt gaatcaggga ataaaattcg gcaggcagat aaacataatc
     1861 ggacatcttg ttcagaacac aaaaaaatgg agcgaaagcc gttacgactt tgaaaatttc
     1921 attctgacgg ttcagggatt gtgccggaaa tcggggattg aaataaaaat tcaaaacccg
     1981 cataaatata gaattgacac tattcccgat gctgatatta tgaaccctgt tattgatgag
     2041 atagtttcaa atttgaaaat gcacggcagc agggagtttg aactggcggt cgtcagtaac
     2101 acagagatgg ttttcagaaa tgattttgac tgttcattca attttgaaag accgaaggcc
     2161 ttactgcgct gtccgttcac aaaaaggaca aacagcccgg gatcaggact tggattgttt
     2221 atattttcgc tttcttcggt tagaggaggt tttgattggg acatatcagt aaaagaaaat
     2281 caattctgtt tgtcattgtt tttctgacaa acggttttct tcttggagag ggctatttta
     2341 atcctgacaa tgatcctatc tggtcagaca cctctttcgg agtaagggca agagaacttt
     2401 cacattctat cgacacgcaa aactgtggaa agggatgcgg tgcactgctc agtcataact
     2461 cgcaatatct tttcaatccg gcaaacgcca tgcttcagca acagttcacc ctttctcttc
     2521 tctatcacta ccttgattcg atgagcgctt ccgtcagtga ttccaagaca tcaaaagttg
     2581 ccggcggagt aatgtattca aggcatgcag gactcaacca tatcaaaact aattttgcat
     2641 tccccatata taagatcgta ttcggcggac taaatgttaa caactatata ggaaaatttg
     2701 ctccgacaca gacaagaagc accaattcac actcatttga tctgggaata agtgctgtga
     2761 taacaaaatt cctttatgta ggtttcggag cgctcgatgt ctggacattt tcaggcaaag
     2821 atattccaag aaaactgaat tggcagtttg aacttaattt taataacatg tttttcttca
     2881 ataacggctg gcaatatcat cttgaaccgg aaaaagagaa gttccccaac aagagaagca
     2941 aactgaaaga ttttgatttc tatacaggtt ttgagttcag atactcgtgg ttcagagcgg
     3001 caatcggatt caacaacctc tctttcaacg aagattttac atgggataca acgcttaaaa
     3061 cattcagcat tgcactatat aaaccgggag ccggcggaat ttacggaagt ttcatgtata
     3121 acgaagctga atatatgact ttcacaataa atatcgtctg ggaaccacct gttcagtgat
     3181 caattcttct ttccgagccg gctgagcatt gtcttgtagc cgggaagatc agcaatatct
     3241 atgtttatat cggcgaatgc gctgttaagt atctcattct tcttttctga agggatcttt
     3301 gacgaaagag ctgaaaagag catttcatat ctgaatccgt tgaacacaaa aaatgaaaga
     3361 taggggctat tgctccatat tgcgtgctgc agggttccgt ccttttcaag agccgttatg
     3421 agatattccc ttccgtcaac tgcaatgttc atccagtctc cgctccacaa tgtaaggact
     3481 tttgcggcat tgcttttcac aatgacacgg cagtttttga cctcgaccgg ctcgtatgca
     3541 ttgacaatta tattcactcc gttccctgcc gctttttcaa gcatcggtgc aattttagca
     3601 agcatcggcg ggaaagcgtc aactattaca gaatgccatg cgttttcaag cattttcgaa
     3661 cacttttcaa gaacctgatc gagcgtctgg agcatataga tcttgtcacc ggacaaagaa
     3721 tagttaatgt ctctgagcga ctcttcaaga aattttctat cgtttctaag tccgctctca
     3781 agctggtcaa gataatctga aaccggtata gccgcatact gctttacagt aggagtttcc
     3841 tctgcaacaa ctgcgccttt tttctccaat gattcaagtg ccttataagt gttagccgcc
     3901 gctttgttca atatctgggc gatcctgtat ccagtcagag gtgattctct cgtcagggtt
     3961 agatatactt ccgcctcaag ccggttgaaa ccgagtttta taagttttgc cacaatagtt
     4021 tcaaaatctg acattttcca cccccttaaa caactttaaa tatcatgggg agtatattta
     4081 tcagtttatt tagagtcaag ggaatcgtat tttttttcgt tggaaaaact ttcttttttc
     4141 cgtttttccc cttttatatt ttccttccaa taaataaaaa tc
//
LOCUS       DAOOYB010000254         4160 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_25281_length_4160_cov_8.727893, whole genome shotgun sequence.
ACCESSION   DAOOYB010000254 DAOOYB010000000
VERSION     DAOOYB010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4160
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25281_length_4160_cov_8.727893"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1331)
                     /locus_tag="PL056_11650"
     CDS             complement(<1..1331)
                     /locus_tag="PL056_11650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPM47715.1"
                     /translation="MSAPKRSEILGSNIVPLMFRLGWPVMLASLLETFYQLADTFWIG
                     RLGGEQSGDSVAALQIAFPLLWFFASAAAGFSMSGTALVSQHTGAGDKSKADIAATQI
                     ISFSLITGIAVAVIGIMFVPELLQLVSSDKNISSIGSSYLVWFFPCMPFVFINASFRI
                     LSAAAGDTKTPLKITLITNIINLIIDPVFIIGWGPIPRMGAAGAAAATLISTALASIA
                     VIYILIKGNTGVSLNFKHIIPEKFWFSRILKIGFPAAVGHSAESLGFILLMTIIAKLP
                     DARNALAGYGIGNRLTSFIFIPAQGLGQGLSTIIGQNLGADNFERARRSAVEGIKIIS
                     ATIAIQIALILPFRESFIALFIPHDAEVIASGAHFLLIFGLSIPFFGVIRGVTSAFNA
                     AGTNVPAMIIGFARLWVLRVPLSWLGGLALGFGATGVWWGMGISNVLAALLS"
     gene            1495..3126
                     /locus_tag="PL056_11655"
     CDS             1495..3126
                     /locus_tag="PL056_11655"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPM47716.1"
                     /translation="MSIATLIVWILGVAGVGATGYIIWSYAKGLKESPKDMWVLFFYM
                     VIQYVAYAAMNPIITLWLSKDCGLGDIAAGNFIMIWSILLSMVGMVAGALVDTIGIRR
                     TMIFSVTFLFIARFFMSFITNPVIVFVVGFLPFAIGFAIVSPVVSVSIKRYTTKEGAA
                     LGFGLFYVIMNLAYAIGGWLMDFVRDKYALRDAAGKIINENAGMELLGIHFSTYQLIY
                     VYALAMTVVSAFFIFFIRDGVEILEETDDEGKKSVKTIIKPVEHHGSGLAAVKGAYLS
                     MFAKIKSAVVEKYFWMFIAILTTTLFVRFIFFHLHYTFPKYGIRVLGEGAKIGNIYGV
                     LNPVLIVFLVPIVASFTKKTKSYTMLVIGSAISSLSCFLAIIPGKVFEGLTNTVLGEI
                     IFIKWLGMADTMEKLMENPPVPEYWSLIFFFLIFTIGEAIWSPRLMQFTAEIAPKGKE
                     GTYIALSILPFFLAKFVVGPMSGLLVNAYTPVVDKIDPATGQVMLDAAGKAMQVVGDI
                     SNHYMVWIWIGGMALVTPIGLLLFKKVFTKNIEGA"
     gene            3141..4160
                     /locus_tag="PL056_11660"
     CDS             3141..4160
                     /locus_tag="PL056_11660"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HPM47717.1"
                     /translation="MEKKIDAMLFDSFYKIAASLTSTLNEEEVLKMIMNLMQEIFQPE
                     NWSLFSYDELTDKLIFRLVVGNEAENLIGRSFNAGKGIAGLSLKSRKPVVIPDATNDE
                     RILKVVPEGSDFVTRSVIAVPMYSKDRPLGVIEMINTDIENFSEEKIKLLEIIADFAS
                     IAVQNANFLKTIEEKATIDDCTDLYNARYMYKILENEISRSKRSGGSFSVVFFDLDRF
                     KNVNDNYGHLIGSQLLREVANVVKACIRPTDYGVRYGGDEFVVILQGADFRNALAVTE
                     RIRETLNSKTFFMKEGYNIKVPASFGLATFPEDAGNIEEIIKAADNAMYKSKETGRNK
                     ISHCH"
BASE COUNT         1214 a          825 c          967 g         1154 t
ORIGIN      
        1 gaaagcagtg ccgcaaggac attgctgatc cccattcccc accacacgcc tgtagcaccg
       61 aatcccagtg ccagtccgcc aagccacgaa aggggaactc tcaggaccca gagtcgggca
      121 aaaccgatta tcattgcggg aacattggtt ccggcggcat taaatgcaga tgtaacgcct
      181 ctgataactc cgaaaaatgg aatggaaagt ccgaaaatga gaagaaaatg ggcgcctgat
      241 gcgataacct cagcatcatg aggaataaaa agagcaatga acgattcacg aaaagggaga
      301 atgagagcta tctgtatggc aatagttgca gaaattattt ttattccttc taccgcggac
      361 ctgcgtgctc tttcaaaatt atctgcaccg agattctgac cgattatcgt tgaaagaccc
      421 tggccgagac cctgagcagg tatgaaaata aagcttgtaa gccggttgcc tatcccatag
      481 cccgcaagag catttctggc atcaggaagc tttgctatta tagtcattaa aagtatgaac
      541 cctaaagatt ccgcactgtg tcccactgcc gctggaaaac cgatcttcaa tattctggaa
      601 aaccagaact tttcaggaat gatgtgcttg aaattaagtg aaactccggt gttgcctttg
      661 atcagaatat aaatgacagc aattgatgca agggctgtgc tgatgagcgt tgccgctgcc
      721 gctcctgccg cacccattct cggtatcggt ccccagccga ttataaaaac ggggtcaatt
      781 ataaggttga tgatatttgt tatcagtgtt attttcagag gggtttttgt gtctcccgca
      841 gcagcggaaa gaattctgaa cgatgcattt atgaatacaa aaggcatgca tggaaaaaac
      901 cagacgagat aggatgatcc gatcgacgaa atgttcttat ccgatgaaac cagttgaagg
      961 agttcaggga cgaacattat accgataacg gcaacagcaa tgcctgttat cagtgaaaat
     1021 gatatgatct gtgttgcggc tatgtcggcc ttagatttat ccccggctcc ggtgtgctgg
     1081 ctcacaagtg ccgttccact catcgaaaaa cctgccgcgg cagaagcaaa gaaccataga
     1141 agaggaaagg caatttgaag agctgcaacc gaatcaccgc tctgttctcc accgagtcgt
     1201 ccgatccaga aagtgtcagc aagctgataa aatgtttcaa gtaaagatgc aagcatgaca
     1261 ggccagccca gacggaacat caacgggaca atatttgaac cgagtatttc agatcgttta
     1321 ggggcggaca tgcaacctcc aaaaagtcga agcattataa caaaaatttt caggaaggaa
     1381 tttttttcag tatttttgag gattaaagac cgaaaaatct tgctaaagac tatgacccga
     1441 ttaatatttt gtgagatgtt tttttgttaa gcttatctct tttcaggagg atttatgagt
     1501 attgcaacgc tgatcgtgtg gattcttggt gtcgcaggtg tcggtgcaac cggttatatc
     1561 atctggagtt atgcaaaggg cttgaaggaa tcccccaagg acatgtgggt ccttttcttt
     1621 tacatggtca tacagtatgt tgcatacgct gcaatgaacc cgataataac actctggctt
     1681 tcaaaggatt gcggattggg tgatattgcc gcgggaaatt ttattatgat ctggtccatc
     1741 cttctttcaa tggttggaat ggttgcgggt gcacttgttg atacaatagg aataagaaga
     1801 acgatgattt tcagtgtcac atttcttttt atcgcaagat tcttcatgtc attcataacc
     1861 aatccggtca ttgttttcgt tgtcggtttt ctcccttttg caattggctt tgcaatcgtt
     1921 tcacccgttg tttcagtgtc gatcaagaga tatacaacga aggaaggtgc ggctctcgga
     1981 ttcggtcttt tttatgtcat aatgaacctt gcttatgcga tcggcggatg gctgatggat
     2041 tttgtaagag acaaatatgc gttgagagat gcggcaggta agataataaa tgaaaacgcc
     2101 ggaatggaac tgctagggat acatttttca acttatcagc tcatatatgt ctatgctctt
     2161 gccatgacag ttgtaagtgc atttttcatt ttctttatca gagacggagt tgaaatactt
     2221 gaagagacag acgatgaagg aaaaaaatct gtaaagacaa ttataaagcc ggtggagcat
     2281 cacggaagcg gtcttgccgc tgtgaaaggt gcgtatcttt caatgtttgc aaaaattaaa
     2341 agtgctgttg ttgaaaaata tttctggatg ttcattgcga ttcttacaac cacacttttt
     2401 gtccggttca ttttcttcca cctgcactat acattcccca aatacggcat cagggtcttg
     2461 ggagaagggg caaaaatagg caatatttac ggtgtgttga atcctgttct gatcgttttt
     2521 cttgttccta tagttgcatc ttttacgaaa aagacaaagt catacacaat gcttgttatc
     2581 ggttccgcga tatcgtcact cagctgtttt cttgccataa ttccgggaaa ggtttttgaa
     2641 gggcttacaa acacagttct tggagagatc attttcatca aatggctcgg aatggctgat
     2701 accatggaaa agcttatgga aaatccgcct gttcctgaat actggtcgct gatattcttc
     2761 tttctcatct tcactatcgg agaagcgata tggtcgccgc ggcttatgca gttcacggct
     2821 gagatcgccc cgaaaggaaa agaggggaca tatatcgccc tttcaattct cccgttcttc
     2881 cttgcaaaat ttgttgtcgg tcctatgtcg gggctccttg tcaatgcgta tacccctgtg
     2941 gttgacaaaa tagatcctgc taccggtcag gtgatgcttg atgcggcagg aaaggcaatg
     3001 caggtcgtag gggatatatc gaatcattac atggtctgga tatggatcgg tggcatggca
     3061 cttgtaactc cgattgggct gcttctgttc aaaaaggtgt ttactaaaaa tattgaaggt
     3121 gcatgaaaaa ggaggttgat gtggaaaaaa agatagatgc aatgcttttt gactcatttt
     3181 ataaaattgc ggcatcactc acttccacat tgaacgaaga ggaagttcta aaaatgataa
     3241 tgaatctcat gcaggagata ttccagccgg agaactggtc acttttttca tatgatgaat
     3301 tgaccgacaa acttattttc cgtcttgtag taggtaatga agcagaaaat ctgataggaa
     3361 gatcgttcaa tgccggaaaa gggatcgccg gactttcact taaaagcaga aagccggttg
     3421 tgatacctga tgcaacaaat gatgaaagga ttcttaaagt tgttccggaa ggcagtgatt
     3481 ttgttacaag atctgtcatt gccgtgccga tgtattcaaa ggacaggccg ctcggagtga
     3541 tagagatgat caacacagac attgagaatt tttcggaaga aaagataaag cttcttgaga
     3601 tcattgctga ttttgcttca attgcagttc agaacgcaaa ttttcttaaa acgatcgaag
     3661 aaaaagcgac cattgatgac tgtaccgatc tttataatgc aagatatatg tacaagatac
     3721 ttgaaaatga gatctcaagg tcaaaaaggt cgggcggttc tttcagcgtt gtctttttcg
     3781 atcttgaccg ttttaagaat gtcaatgaca actatggaca tctgatcggt tcacagcttc
     3841 tgcgcgaagt tgcaaatgtt gttaaagcgt gtatcagacc gaccgattac ggtgtcagat
     3901 atggaggtga cgaatttgtt gtcatacttc agggggcgga tttcaggaac gccctcgctg
     3961 ttactgaaag gatccgcgaa acactcaatt caaagacatt tttcatgaaa gaaggttaca
     4021 acataaaagt tcccgccagt ttcggacttg caacatttcc cgaagacgcc ggaaatatag
     4081 aagagatcat aaaagcggcg gacaacgcca tgtacaaatc aaaagaaacc ggcagaaaca
     4141 agatcagtca ctgccattaa
//
LOCUS       DAOOYB010000255         4146 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_25393_length_4146_cov_11.517722, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000255 DAOOYB010000000
VERSION     DAOOYB010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4146
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25393_length_4146_cov_11.517722"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /locus_tag="PL056_11665"
     CDS             <1..876
                     /locus_tag="PL056_11665"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HPM47718.1"
                     /translation="ALGGKERKKILSIASAIELLHAGFLMHDDVMDGDILRRGEPSVH
                     VSYRDLFADEGLLNPEKNGESFAICLGNIAYFMAFDLIAGSGFDTATAIRLIRIFNRE
                     AVITGLGQMNDIFTGALKDFPDERSILDTYRMKTARYTFSLPLIMGSVCAGLDDELIE
                     KIVEPAEAIGLVFQMRDDELNILEDPQGTGKSAGSDITEGKKTLIISMLLKNIEGDDK
                     RKMEKIFSKKELTLNELEYIRKALADSGTVDEHRKMISQISLSAKEAIESLDINEEYK
                     NILRYILKISVERKF"
     gene            complement(878..1792)
                     /locus_tag="PL056_11670"
     CDS             complement(878..1792)
                     /locus_tag="PL056_11670"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPM47719.1"
                     /translation="MKRFIFTTHDGAEISAAKWIPDSEIKAVVQIVHGMNEYIERHSK
                     TAEFLENNGYAVFGEDHRGHGLTVKDGNFGHFDNELGYKKVIEDNYTLTKLIKNEFPD
                     NKVFLFGHSMGSFISRNYVFEHGNEIDGLLLMGTGGLPLKNKIVNLTTLKVLAPFVKP
                     DTKLPLVHRIGNADLNKAFRPGITGFEWLSTDSSVSEKFLADPMCGNVNTYAFYKDLV
                     TMMLEMERAENIAKIPKKLPVFIFSGNMDPVGDFGRKVTQTYKDYIKAGLIDVTIRLY
                     DGYRHELHNEPVRDELFKEILNWLEKRR"
     gene            complement(1792..2349)
                     /locus_tag="PL056_11675"
     CDS             complement(1792..2349)
                     /locus_tag="PL056_11675"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018280324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HPM47720.1"
                     /translation="MTKKIIINKNTVLKQIELTDTADIFKTIISQREYLGKWLPFVEF
                     TKEIKDTENFVRSVVDTPEKIREFVFVIHSDGKFAGLAGFKDTDRLNRKTEIGYWLSE
                     GFQKKGIMTESVKLLVKLAFEELNINRIQIRCAVGNTASSNIPKKLGFNFEGIEIEGE
                     LFPDGSFKDLEVYSILKSMSRKEEK"
     gene            complement(2365..3858)
                     /gene="glpK"
                     /locus_tag="PL056_11680"
     CDS             complement(2365..3858)
                     /gene="glpK"
                     /locus_tag="PL056_11680"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016523127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HPM47721.1"
                     /translation="MKNYILSIDAGTTSSRAIIFDRSSAVVAMSQKEFSQIYPKPGWV
                     EHDANEIWNTQLAVIKQVVDDSGISPDEIASVGITNQRETTVVWNRHTGKPVYNAIVW
                     QCRRTADMCRKLKENGYEEMIKSSTGLVVDAYFSATKLAWILENVPKARRDAEKGDLI
                     FGTIDSWILFNLTKGKVHATDVSNASRTMMFNIEKLDWDDNILNILNIPRSMLPKVSP
                     SSYDFGATAEGIFDNVQIPICGIAGDQQASLFGQVCFKKGAAKNTYGTGCFLMMNTGH
                     DIVRSDEGLLTTIAWQIGDEVTYALEGSIFIGGAVIQWLRDEMKMLDNAKESEYFATQ
                     VKDNGGAYIVPAFVGLGTPYWDPYARGTITGLTRGIGKNHIIRAALESICYQTKDVFE
                     AIHDECGLNLTELKVDGGACSNNFLMQFQSDMLGVPVYRPVVIETTALGVAYLAGLYA
                     GMWKNTAEISKNWKVEKIFGSLMSAEERINLYKYWKKAVDRSKAWEE"
BASE COUNT         1142 a          890 c          811 g         1303 t
ORIGIN      
        1 gctctcggtg gaaaggaaag gaagaaaatt ctttcaatcg cttctgcgat agaacttctg
       61 catgcgggtt ttctcatgca cgatgatgta atggacgggg acattttgag aaggggagaa
      121 ccctcggttc atgtttcata cagagatctt tttgccgatg aaggtctttt aaatcctgaa
      181 aagaacggag aatcttttgc gatatgtctt ggtaacattg catatttcat ggcctttgat
      241 cttattgcag gatcgggctt tgatactgca accgcaatcc ggctcataag gatattcaac
      301 cgcgaagcag tcattacagg tctgggtcag atgaatgaca tttttaccgg cgcactgaag
      361 gattttcctg atgaaaggtc gatacttgat acctacagaa tgaaaacggc aagatacact
      421 ttctctcttc ctctcataat gggttcagta tgtgcaggtc tggacgatga gctgatagaa
      481 aagatagttg agccggctga agccataggg cttgtttttc agatgagaga tgatgaactt
      541 aatattcttg aagatccgca ggggacagga aaatcggcag gaagcgatat tacggagggg
      601 aagaaaacat taataatatc aatgctttta aaaaatattg aaggtgatga taaaagaaaa
      661 atggagaaga tcttttcaaa aaaagagctc actctgaatg aacttgagta catcagaaaa
      721 gctcttgcgg attcaggcac tgttgatgaa catcgaaaaa tgatatctca aatatcccta
      781 tcggcaaagg aagcgataga atctcttgat atcaatgaag agtataaaaa tattctcaga
      841 tatattctta agatctcggt tgaaagaaaa ttctgaacta tcttcttttt tccaaccagt
      901 taaggatctc tttaaaaagc tcgtctctaa ccggttcgtt atgcagttcg tggcgatagc
      961 catcgtaaag tctgatagtt acatcaatca gtccggcttt tatataatct ttataagtct
     1021 gagtcacttt tctgccgaaa tctccgacag ggtccatgtt tcctgaaaaa atgaaaacgg
     1081 gcagtttttt aggaattttt gcaatgtttt cagccctttc catttcaagc atcattgtca
     1141 caagatcttt ataaaatgcg taggtgttga catttccgca catcgggtca gctaaaaatt
     1201 tttcagaaac cgaagaatcg gtgctcagcc actcaaatcc ggtaattcca ggtctgaaag
     1261 ctttgttgag atcagcattt ccaatcctgt ggacaagtgg aagtttggta tcaggtttaa
     1321 caaaaggggc aagaactttc aaagttgtca gattcactat cttattttta agcggcagcc
     1381 cccctgttcc catgagaaga agtccgtcaa tttcgtttcc gtgttcaaaa acatagtttc
     1441 ttgaaatgaa tgatcccatg ctgtgaccga acaggaacac tttattatcg ggaaactcat
     1501 ttttgataag ttttgtaagt gtgtagttgt cttcaatgac ttttttgtat cctaattcat
     1561 tgtcaaaatg gccgaagttg ccgtctttga cagtcagacc gtgaccgcga tggtcttcac
     1621 caaaaactgc atatccgtta ttctcaagga attcagcagt ttttgaatgt ctttcaatgt
     1681 attcgttcat tccgtgaact atctgcacga ccgccttaat ttcactgtca gggatccatt
     1741 ttgccgcgct tatttcagca ccgtcatgag tagtgaatat aaatcttttc atcatttttc
     1801 ctccttccgg gacattgatt tcaatatact gtaaacttca aggtctttga atgagccatc
     1861 ggggaaaagt tcaccctcta tttcaattcc ctcaaaatta aatccgagtt ttttcgggat
     1921 gttgctgctt gcagtgtttc cgacagcgca tcttatctgg atgcggttta tgttgagctc
     1981 ttcaaacgcc agttttacca gcagcttaac agattcagtc ataatccctt ttttctggaa
     2041 cccttcactg agccagtaac ctatctcagt ttttctgttt aatctgtctg tatccttaaa
     2101 cccagctaaa cccgcaaatt ttccgtcaga atggatgaca aaaacaaatt cacgaatctt
     2161 ttcaggggtg tccacaacag atcggacaaa attttcagta tccttaatct ctttggtaaa
     2221 ttcaacaaac ggaagccatt ttccaagata ttcccgctga gaaattatcg ttttaaaaat
     2281 atccgccgta tcggtcagct ctatctgctt taaaaccgtg tttttgttga tgattatttt
     2341 ttttgtcatg gtttactccg ccatttattc ttcccacgct tttgatctgt caacggcttt
     2401 tttccagtat ttgtaaaggt ttatcctttc ttcagcagac atgagagagc cgaagatttt
     2461 ttcaactttc cagttttttg agatctctgc ggtgtttttc cacattccgg cataaagacc
     2521 tgcaagatag gcaactccca gagcggttgt ttcgatcacg accggtcggt aaactggaac
     2581 tccgagcatg tcactttgaa actgcatcag aaagttgttt gaacacgctc cgccgtcaac
     2641 tttaagctct gtcagattga gtccgcactc atcgtggatc gcttcaaaaa catcctttgt
     2701 ctgataacag atcgattcga gagcggctct gatgatgtgg tttttgccga tcccccttgt
     2761 aagtccggtt atcgtccctc ttgcataagg atcccagtaa ggtgtgccga gtcccacaaa
     2821 agcgggaacg atatatgctc cgccgttatc tttgacttgt gttgcaaaat attcactttc
     2881 cttagcatta tcaagcattt tcatttcatc ccggagccac tgtatgacag ctccgccgat
     2941 aaatatcgac ccttcaaggg cataagtcac ctcatcaccg atctgccatg cgatcgttgt
     3001 gagaagccct tcatctgaac ggacaatatc gtgaccagtg ttcatcatga gaaaacaacc
     3061 cgttccgtaa gtgttctttg ccgccccttt tttaaagcag acctgaccga ataatgatgc
     3121 ctgctgatca cctgcaatgc cgcagattgg tatttgcaca ttatcaaaaa taccttctgc
     3181 cgtggctccg aaatcatagc tcgaagggct gactttcgga agcattgatc ttggaatatt
     3241 caaaatattc aatatgttat cgtcccagtc gagtttttcg atgttgaaca tcatggttct
     3301 tgaagcgttt gacacatcgg tcgcgtggac tttccctttt gtcagattga aaaggatcca
     3361 cgaatcaata gttccgaaaa tgagatctcc tttttcggca tccctgcggg cttttggaac
     3421 attttcaagg atccatgcaa gctttgttgc tgaaaaatat gcgtcaacaa ctaaacctgt
     3481 tgaagatttt atcatttctt catacccgtt ttccttaagt tttctgcaca tatcggcagt
     3541 tcttctgcac tgccagacaa ttgcgttgta aaccggtttc ccagtgtgcc tattccacac
     3601 cactgtcgtt tcacgctgat tagttatccc gacagatgcg atctcatcag ggcttattcc
     3661 tgaatcatca acgacctgtt ttatcactgc aagctgagtg ttccatatct cattcgcatc
     3721 gtgttcgacc cagcccggct tcggatatat ctgagagaac tccttttgag acattgcgac
     3781 aacagcactc gacctgtcaa aaattatcgc ccttgaactc gtcgttcccg catcaatcga
     3841 aagaatgtaa tttttcacac ttcctcccca aaattaaaat cctgcgaata gtaacatgtt
     3901 agacagaagt tgtaaaattt tgggaatttc ctacacctta aaaaatgaca cctatttata
     3961 aatgaacatt tgaacaatcg ttcaagtaag tgtctgagct atttgactaa aacaggtttt
     4021 tgagataatc tgactcgtta attatttgaa tttttagggt tttatgaagc gaacgattct
     4081 gattttcaca ctttttttga tttcatgtaa ttcaaatgac cctaaaacgg atgcggataa
     4141 agctgt
//
LOCUS       DAOOYB010000256         4122 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_25605_length_4122_cov_11.034423, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000256 DAOOYB010000000
VERSION     DAOOYB010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4122
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25605_length_4122_cov_11.034423"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(219..557)
                     /locus_tag="PL056_11685"
     CDS             complement(219..557)
                     /locus_tag="PL056_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HPM47722.1"
                     /translation="MKVAIPLVNGMLCMHFGHCAQFAIIDVDDKEKKIVKREDVTPPP
                     HEPGLLPKWLGEKNVNVIIAGGMGSRAQTLFAQNNISVIVGSPAQTPEKLVTDYLNGQ
                     LVSGTNVCDH"
     gene            complement(554..1435)
                     /locus_tag="PL056_11690"
     CDS             complement(554..1435)
                     /locus_tag="PL056_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPM47723.1"
                     /translation="MKEIVVISGKGGTGKTSIASSLFALSQNSIAADCDVDAADMFIV
                     LEPEIVRKSDFYSGRVAVIDNNKCTNCGVCRELCRFDSISVRSGVHFIDGNECEGCKV
                     CVEFCPEKAIDFIDRLCGEWFFSETRFGPLVHAKLGIGAENSGKLVTLVRNEAKRIAV
                     EKKSDFIIVDGPPGIGCPVIASVTGADAVLIVTEPTLSGFHDLKRVMSLAKHFRIKTA
                     VVVNRWDVNSEMTDNIEEYVHGEGASFIGRVSYSSDFTDAQILKKTVVEYKNGMTYEE
                     IHSIWKKLLKFVNGEEK"
     gene            complement(1432..2277)
                     /locus_tag="PL056_11695"
     CDS             complement(1432..2277)
                     /locus_tag="PL056_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006963874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM47724.1"
                     /translation="MKVAVASGKGGTGKTTVSIALAMTSPGMVQLLDCDVEEPNGHLF
                     LELENVIEERVSVDVPLINTDTCISCGKCAQICQFNAIVFFGSPPMVFNELCHSCGGC
                     MRVCPVSAITEVPNFIGKIVSGRSGRIDFSQGILDIGFPMATPVIRKVLKKADGNNDV
                     VIDSPPGTSCSMIAAVKESDFVILVTEPTPFGLNDLKIAVETIKKLSIPVGVIINRSM
                     AGDDLVEKYCEDNNLKILMKIPYSHKIAKICSTGGNLITGAPDLKKKLEDLWKFIFWT
                     TGGEK"
     gene            complement(2277..2636)
                     /locus_tag="PL056_11700"
     CDS             complement(2277..2636)
                     /locus_tag="PL056_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HPM47725.1"
                     /translation="MKIAVTTTGNDLNSPFENRFGRAPGFIIYDTETKQFEAVDNSMN
                     LNAAQGAGIQAAMNVIKAGAKVLVSGHCGPKAFTVLKQAEVKIFNANAATVSEAVEKY
                     VSGMLIEMKSADVDGHW"
     gene            complement(2633..3019)
                     /locus_tag="PL056_11705"
     CDS             complement(2633..3019)
                     /locus_tag="PL056_11705"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HPM47726.1"
                     /translation="MKVLITIWNGRISPVFDVAKEALLINVENGEVVSKSSVSIVYGT
                     CMHKVDFIISEKIDVLICGAVSRRVEMELIGRGVIVYSFVSGDIDEVITGFIHNRLAK
                     PGFAMPGCGRGKRCRIFNIRNMEHKK"
     gene            3298..4122
                     /locus_tag="PL056_11710"
     CDS             3298..4122
                     /locus_tag="PL056_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HPM47727.1"
                     /translation="MSFSLFTNPEEEKRFNDTVSKIKNIIVVMSGKGGVGKSSVAVNL
                     AYYFAMQGKNVGIMDVDFHGPSIPKLLGIEGKKLGSDESGRIKPVRISDTFHAVSIGL
                     ALDSIDSPVIWRGPMKVSAIKQFFCDVEWGELDYLIIDSPPGTGDEPLTVAQFLQNKA
                     NALIVTTPQEVSISDVRKSLSFASGLGFKNLGIVVNMSHFVCPKCGEKSYIFKQGSIA
                     KMAEDHNTEILGEIPIEPLVSECGDEGRFFVNVYGKTPAGIEFEKIGEKIFKKLAE"
BASE COUNT         1173 a          958 c          746 g         1245 t
ORIGIN      
        1 ttcatcagtg tggtctttcc gcttccgctt gcacccttca ggcgaagcac cgctccgctt
       61 ttaagctcaa ggacaagatc cttaataact gcaaaactct ctccagttgc atccttgtaa
      121 ctctttgaaa ttcctttaat aataacttcc atatgtcctc cgccggcaaa tcttgaaagc
      181 tgaaaacctt acccgttaat aaattttaag tcaatgaatt aatggtcaca cacattagta
      241 cccgacacaa gctgaccgtt taaataatcg gtaacaagct tttccggtgt ttgggctgga
      301 gaacctacta tcactgaaat attattctgg gcaaaaagtg tctgagctct tgaacccatg
      361 ccgcctgcaa taataacatt cacatttttt tcaccaagcc atttgggaag gagtcccggc
      421 tcatgtggcg gaggagtgac atcttctctt ttaacaattt tcttctcttt gtcgtcaaca
      481 tcaatgatag caaattgagc acaatggcca aaatgcatgc acaacatgcc gtttacaagc
      541 ggaatcgcta ctttcatttt tcttctccat ttacaaattt taataatttt ttccatatag
      601 aatgaatttc ttcgtatgtc atcccatttt tatattccac taccgttttt tttaagatct
      661 gtgcgtctgt aaaatcactg ctgtatgaaa cacgcccgat aaaagaagca ccttcgccat
      721 gcacatattc ttcaatatta tcagtcattt cactattcac atcccatctg ttaaccacta
      781 ctgccgtctt tattctaaag tgtttggcaa gcgacataac cctcttaaga tcatgaaaac
      841 cgctcagtgt cggctcagta acaatgagca ctgcatcagc acctgttaca gaagcaatga
      901 caggacaacc tataccgggc ggcccgtcaa caattataaa atctgatttt ttctctacgg
      961 ctattctttt tgcctcgttt cttacaagtg tgacaagttt ccctgaattt tcagcgccga
     1021 ttccgagttt tgcatgaaca agcgggccga aacgggtttc agaaaaaaac cactctccgc
     1081 acaatctgtc aataaaatca atggctttct cagggcagaa ctctacacat actttacacc
     1141 cttcacattc atttccatca atgaaatgca ctcctgatct taccgagatc gaatcaaatc
     1201 tgcaaagttc acggcatact ccgcaatttg tacacttatt attatctatt acagcgactc
     1261 ttccactgta gaaatcactt ttcctgacaa tttccggttc aaggacaatg aacatgtctg
     1321 cggcatctac atcacaatca gcggcaatac tgttttgcga aagggcaaaa agtgacgaag
     1381 caatgctggt tttccctgtt cccccttttc cgcttatgac aacgatctct ttcatttttc
     1441 gcctccagtc gtccagaaaa tgaatttcca gagatcttca agcttttttt tcagatcagg
     1501 agcgccggtt attaaatttc ctccggttga gcatattttt gcgattttat ggctgtatgg
     1561 tattttcatc agtattttca aattgttatc ttcacaatat ttttcgacta aatcatctcc
     1621 cgccattgat ctgtttataa ttacgcccac cgggattgaa agctttttta ttgtctccac
     1681 agctatttta aggtcattga gtccaaaagg tgtcggttct gtgacaagaa tgacaaagtc
     1741 gctctctttt acagccgcga tcattgaaca gctcgttccg ggaggagagt ctatgacaac
     1801 atcattgttt ccgtctgctt ttttcagaac ttttcttatc acaggtgtcg ccatcggaaa
     1861 acctatatca agaattccct gggaaaaatc aatacggccg cttctgccgg aaactatctt
     1921 ccctatgaaa ttgggcactt cagttattgc tgaaactgga cagaccctca tgcagccacc
     1981 gcaactgtga caaagctcat tgaacaccat aggaggtgaa ccgaaaaaca ctatcgcatt
     2041 gaactggcat atctgagcgc atttcccgca actgatacat gtgtctgtat ttattaaagg
     2101 aacatccacc gaaactcttt cttcgatcac attttcaagt tcaagaaaaa gatgaccgtt
     2161 cggctcttca acatcacagt caagcagctg aaccattccg ggagatgtca ttgcaagggc
     2221 gatggagacc gttgtcttac cggtcccccc tttacctgaa gcaactgcta ctttcattac
     2281 cagtgaccgt caacatcggc agatttcatc tcaatcagca tgcctgaaac atacttttca
     2341 acagcttccg acactgttgc cgcatttgcg ttgaatatct ttacttccgc ctgcttaaga
     2401 actgtgaagg ccttcggtcc gcagtgacct gagacaagga ctttcgcacc tgcctttatc
     2461 acattcattg ccgcctggat ccctgctccc tgagccgcat tgaggttcat tgaattatca
     2521 actgcttcaa actgttttgt ttcggtatca taaataataa accccggtgc tcttccgaat
     2581 ctgttttcaa acggtgaatt cagatcattt cctgtagttg taactgctat tttcattttt
     2641 tatgctccat atttcttata ttaaaaattc tgcaccgttt tccccttccg caacccggca
     2701 ttgcaaaacc cggtttagca agacggttat gaataaagcc cgttatcact tcatcaatat
     2761 cacccgaaac aaacgaatac acaataactc ctctgccgat aagttccatc tcaacccttc
     2821 ttgaaacagc tccgcaaata aggacatcga tcttttcact tattatgaaa tcaactttgt
     2881 gcatgcaggt tccgtaaacg attgaaacag agctctttga aacaacctcg ccgttttcaa
     2941 catttatcaa caacgcctct tttgcaacat caaaaaccgg agaaattctc ccgttccaga
     3001 ttgttataag aactttcatc aattcactcc aaaaaaaacc ggtccttaat tgcaattgtt
     3061 gtgccaataa caacagaaac ggtcaaccat ctgtttttac ttatttttat tggtttttag
     3121 attcttgaac acaacagaat gagttgcatt attgcaactt tattttgctg ttttgtggca
     3181 gttttgcaac tttgttgcgt caaagtgaac attgcttaaa actcccctct tgaaaaaaaa
     3241 gattttgggg actattgtct ctgtgtgcat gtttttttga tatggaggaa ttagaatatg
     3301 tcattttcac tttttacgaa tcctgaagaa gaaaaacggt ttaacgacac tgtttcaaag
     3361 attaaaaaca ttattgttgt gatgagcgga aaagggggtg tgggcaaaag cagtgtggct
     3421 gtgaaccttg catattattt tgcgatgcag gggaaaaatg tcggcatcat ggatgttgat
     3481 tttcacggtc cgagcattcc caaacttctt ggaattgaag ggaaaaaact gggatcagat
     3541 gaatcgggaa gaattaagcc ggtaagaatt tcagacactt ttcacgctgt aagcatcggg
     3601 cttgcacttg attccattga ctcgcctgtc atctggagag ggccgatgaa agtaagtgcg
     3661 atcaaacagt tcttctgcga tgttgagtgg ggcgaactcg attatcttat cattgactcc
     3721 cctcccggaa caggtgacga accgcttact gtcgcacagt tccttcaaaa taaagcaaat
     3781 gcattgatag taacaactcc tcaggaagtt tcaataagcg atgtcagaaa atcactttca
     3841 tttgcaagcg gacttggatt taaaaatctc ggaatagtcg tcaacatgag ccactttgtc
     3901 tgccccaaat gcggtgaaaa aagttatatc ttcaaacaag gcagcattgc aaaaatggca
     3961 gaggatcaca acactgaaat tcttggcgaa atcccgatcg aacctttagt cagcgaatgc
     4021 ggcgatgaag gaagattttt tgtcaatgtt tacgggaaaa caccagccgg aatcgaattt
     4081 gaaaagatag gtgaaaagat ctttaagaag cttgccgaat aa
//
LOCUS       DAOOYB010000257         4116 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_25663_length_4116_cov_10.096528, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000257 DAOOYB010000000
VERSION     DAOOYB010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4116
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25663_length_4116_cov_10.096528"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..964
                     /locus_tag="PL056_11715"
     CDS             <1..964
                     /locus_tag="PL056_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012447706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HPM47728.1"
                     /translation="GAGAFVCGEETAMINSLEGKRGTPRFRPPFPTDSGYKGYPSLIN
                     NVETFANIPVLINEGGESYALIGTQGSKGTKLFALAGDIKNPGLVEVPMGITIGEIVY
                     DLGGAEKGSVKAVQIGGPSGGCIPEDKFDISVDYDSLRSLGAIMGSGGLIAVGKNRCM
                     VETARYFLDFTAKESCGKCTFCRVGTKRMLETLELITKGKGTLEHVEFLEDIGPKIIK
                     GSLCGLGQSAPNPVISTLRFFKHEYLEHVTDKKCSALICNALVELKIDKDKCIKCKLC
                     IKACPVNAISDDFIVNNSLCTRCNSCIEVCPKDAVGRIPAGGAK"
     gene            961..2988
                     /locus_tag="PL056_11720"
     CDS             961..2988
                     /locus_tag="PL056_11720"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPM47729.1"
                     /translation="MKMINLKINGTQISVPENTTILNAAKTLGIEIPTLCFNEKITHN
                     TSCFVCVVKDLKTGKFTPSCAARVAEGMEIDAGSDEVLDMRRTCLNLLLSEHRGDCEA
                     PCTIACPAHAMVEEYVRLGKEGKHLEGLKVIKERISLPMSIGRVCPRFCEKECRRNVA
                     DRPVAINEFKRTAADLHYGEYIEEMKPINGKKVAIVGAGPAGLSVAYFLRLEGVASEI
                     FDMNPKAGGMLRYGIPEYRLPKDILDLEISHFNKMGGINFHFSKKLGTDFTVEELEKK
                     YDAVAICIGSWKSSSAQVKGEEFTEGGIQWLENIAENGWSGKNPGKVIVIGGGNTAMD
                     CLRTSVRLGSSDVNCFYRRTEKEMPAEKIEIHEAKEEGVNFHFLVAPVSVREENGKKI
                     LTCQKMELGAPDASGRRRPVPVEGSDFDTVADLVIGAIGQKTDAPAGVPVNKWGDVAV
                     NEKNCHAGGKLFAAGDCVSGPATVVEAVAGARRVAMSILSFFEGREYSEPYLINVSRG
                     HWNYLKKEDLVFVGKPENYDRREQQLISIEERKTTFKEVAKSFTVEELATEGKRCYEC
                     SCTDKHDCKLKKYSELLHCSPDLIKGEKIEIKYDARHPEIILDPGKCIKCGVCIKLSK
                     EIVNNQILTFKFRGFSTTIGTALDAAVDSPLEELKLYVENCPTGALAIKNK"
     gene            2981..3967
                     /locus_tag="PL056_11725"
     CDS             2981..3967
                     /locus_tag="PL056_11725"
                     /inference="COORDINATES: protein motif:HMM:NF018009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HPM47730.1"
                     /translation="MSKGERFFAVTQKLSDDLQNELKGLRGFSSTNIKRMKQFYEAWE
                     AYFLISPLSTDQLENNSDKTRIIENSPLPTDQLKLEKSSLMTNQFVEKFLLVSFTAHY
                     EIISKVKKLDGRIFYIVKNAEEFWSVEALKHKIKNNLFEKEKKLPANFKKTLPPEMTH
                     TALASFRDHYLLDYIALSDEENESERVLESEIVHNIKKFMLSLGSDFSFISNQYRIIV
                     EEEEYFIDLLFYHRGLQALVAFELKTGRFKPEYLGKMNFYLSALDEYVKKEHENPSIG
                     IILCKDKKDTVVEFAFRNVNKAMGVATYETSEKLPEKYRNILPDASVFKNIL"
BASE COUNT         1298 a          729 c          993 g         1096 t
ORIGIN      
        1 aggggcaggt gcttttgtct gcggagaaga gactgcgatg atcaattccc ttgaaggaaa
       61 aaggggaacg ccgagattcc gtccaccttt cccgaccgac agcggataca agggctatcc
      121 ttcgcttatc aacaatgtcg agacttttgc gaacattccg gttcttatta atgaaggtgg
      181 agaaagttat gctttgatag gaacacaggg gtcaaaaggg acaaaacttt tcgcccttgc
      241 cggtgacatt aaaaacccgg gtcttgtcga agttccgatg gggataacga tcggagagat
      301 agtttacgat cttggaggag ctgaaaaagg gagtgtcaag gcggtgcaga tcggaggtcc
      361 gagcggtgga tgtattcctg aagacaagtt tgatatttcg gttgattatg attcattgag
      421 atcgcttggc gcaataatgg gttccggtgg tcttattgct gtcggaaaga accgctgcat
      481 ggtcgagacg gcaagatatt ttcttgattt caccgctaag gaaagttgcg gaaaatgtac
      541 tttctgcagg gttggaacaa agagaatgct tgaaaccctt gaacttatta caaaagggaa
      601 ggggactctt gaacatgttg agttccttga agatatcggg cctaaaatta taaagggatc
      661 tctctgcggg ctcggtcagt cggctccgaa ccctgttatt tcaactctca ggttcttcaa
      721 acacgaatat cttgagcatg tgactgataa gaaatgttcg gcacttatct gcaatgcact
      781 ggttgaactg aaaattgata aagataaatg tataaagtgc aagctctgca tcaaggcgtg
      841 cccggtaaat gcgataagcg atgattttat cgtcaacaac tcgctctgca caaggtgcaa
      901 cagttgtatc gaggtgtgtc cgaaagatgc tgtgggcaga attccggcag gaggtgcaaa
      961 atgaaaatga taaatcttaa aattaacggt acgcagatat ctgttcctga aaataccacc
     1021 attcttaatg cggcgaaaac gcttggaatt gagatcccga cactctgctt taatgaaaag
     1081 ataacacaca atacgagctg ttttgtctgt gttgtgaaag atcttaagac cggaaaattc
     1141 actccgtcat gtgcggcaag ggtcgctgaa gggatggaga tagatgccgg aagcgatgaa
     1201 gttcttgata tgcggcgcac ctgtcttaat cttctgcttt cagagcatag aggtgactgt
     1261 gaagcacctt gcacgatcgc ctgtccggca cacgcaatgg tcgaggaata tgtcaggctt
     1321 ggaaaagagg gaaagcatct tgagggattg aaagtaataa aagagagaat atctcttccg
     1381 atgagtatcg gtcgtgtgtg tccgaggttc tgtgaaaagg agtgcagaag gaatgttgcc
     1441 gacagacctg ttgctataaa tgagttcaaa agaactgcgg ctgatctcca ttacggcgaa
     1501 tatattgaag aaatgaagcc cataaacggt aagaaagttg cgatagtcgg tgccggtcct
     1561 gccggacttt ctgttgcata ttttctccgt cttgaagggg ttgcaagcga gatctttgac
     1621 atgaacccta aggccggcgg aatgctcaga tacggaattc ctgagtaccg tcttccgaaa
     1681 gatattcttg atcttgaaat atctcatttt aataagatgg gtgggataaa tttccatttc
     1741 tcaaagaaac tgggaactga ttttactgtt gaagagcttg agaaaaaata tgatgcagtt
     1801 gcaatatgca taggaagctg gaaaagttca agtgctcagg ttaaaggaga ggaatttaca
     1861 gaaggcggca ttcaatggct tgaaaacatt gctgaaaatg gatggtcggg caaaaatccc
     1921 ggtaaggtga tagttatcgg cggcggtaac actgcaatgg actgtcttag aacttcagta
     1981 cggcttggaa gcagtgatgt caactgtttt tacagaagga cagagaaaga gatgcctgct
     2041 gaaaagatcg aaatccacga agcgaaagaa gaaggggtca attttcattt tcttgtcgct
     2101 ccggtaagtg tgagagaaga gaacggcaaa aagattctta cctgtcagaa gatggagctt
     2161 ggtgctcctg atgcatcagg aagaagaagg ccggtccctg ttgagggaag cgattttgat
     2221 actgttgctg accttgtgat tggtgcaatc gggcagaaaa ctgatgctcc ggcaggggtt
     2281 cctgtgaata aatggggtga tgttgcggta aatgaaaaga actgtcatgc aggtggaaaa
     2341 ctttttgcgg ctggggattg tgtaagcggt ccggctactg tagttgaagc ggttgcaggg
     2401 gcaagaaggg tggcaatgtc tattcttagc ttttttgaag gcagggaata cagcgagccg
     2461 tatctcatca atgtttcaag aggtcactgg aactatctta aaaaagaaga tcttgttttt
     2521 gtcggaaagc ccgaaaatta cgacagaaga gagcagcagc taatttcaat tgaagagaga
     2581 aagacaacat ttaaagaagt tgcaaaatct ttcacagttg aagaacttgc tactgaagga
     2641 aagcggtgtt acgaatgttc ctgcaccgat aagcacgact gcaaacttaa aaagtacagc
     2701 gaacttcttc attgctcacc tgaccttatc aaaggggaaa agatcgaaat aaaatacgat
     2761 gcaagacacc ccgaaataat ccttgatcca ggcaaatgca tcaaatgcgg agtctgtatc
     2821 aaactcagca aggaaatcgt taataatcag atacttacat tcaaattcag gggcttttcc
     2881 acaacaatcg gaaccgctct tgacgcagca gtcgattcac cccttgaaga actcaaactc
     2941 tatgtcgaga actgccccac cggagcactc gcaataaaga ataagtaagg gcgaaagatt
     3001 ttttgctgtc actcaaaaac tttcagatga tctgcaaaat gaactcaaag gactgagagg
     3061 tttttcttca acaaatatta agcgaatgaa acaattttat gaagcttggg aagcatattt
     3121 tttaattagt ccgttgtcaa cggaccaatt ggaaaacaac tcagataaaa cgagaataat
     3181 tgaaaatagt ccgttaccga cggaccaatt gaaattggaa aaaagttcgt taatgacgaa
     3241 ccaatttgtt gagaagtttc tgcttgtgag tttcactgct cattacgaaa taatttcaaa
     3301 ggtaaaaaaa ctcgatggaa ggatttttta tatcgttaaa aatgctgagg aattctggag
     3361 tgtcgaagcg cttaaacaca agataaagaa caatcttttt gaaaaggaga aaaagcttcc
     3421 ggcaaatttt aagaaaacgc tgccacctga aatgacacac acggccctgg cgtcatttcg
     3481 cgaccactat ctacttgatt atattgcgtt atctgatgaa gaaaatgaaa gcgaaagagt
     3541 tcttgaaagt gaaattgttc acaatattaa gaaattcatg ctgtctctcg gatcagattt
     3601 ttcctttatt tcaaaccagt acagaataat tgtggaagaa gaagagtatt ttattgatct
     3661 gcttttttat cacagagggt tgcaggcact tgttgcattt gagcttaaaa caggcagatt
     3721 taaacctgaa tatctgggaa aaatgaattt ttatctttca gcacttgatg aatatgtgaa
     3781 gaaagaacat gaaaatcctt caatcgggat aattctctgt aaagataaaa aagatacagt
     3841 cgttgagttt gcatttagaa atgtcaataa agcaatggga gttgccactt atgaaacaag
     3901 tgaaaaactg cctgaaaaat accggaacat tctgcctgat gcgtcggtat ttaaaaatat
     3961 tttataattt tttcgagtat atttatattt cacgcttcca catttccaca tttacacggg
     4021 cgaacacatg ggttagcccc tacaaataat ttgatatctc actcttaaag tttagaataa
     4081 tcgggattgg aattggaaaa tggatgtttt tatatt
//
LOCUS       DAOOYB010000258         4078 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_25974_length_4078_cov_10.041263, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000258 DAOOYB010000000
VERSION     DAOOYB010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4078
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25974_length_4078_cov_10.041263"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..762
                     /locus_tag="PL056_11730"
     CDS             <1..762
                     /locus_tag="PL056_11730"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HPM47731.1"
                     /translation="TEGKCKGYQTVADTSEMMEIPAGKYYVGSYSPPADLIDPDERPW
                     ESWIAYYENYLPLYYIDKYEVTQKDYSECVEAGICSEPVKKRSGWRSDYWGNSEYDNY
                     PVLYVNYYEAEKYCKWRGKRLPTEFEWVKAANGGEMRWYPWGDVDPDSDNRFGNIFYS
                     LNETKSYDTAEVGTYPLDISPFGVMDLNGNVSEWTASNWTRMLGYEATYGDKLKVVKG
                     GRWEENSNYSGWIAQRMAATPWTSSNTVGFRCVKD"
     gene            922..1347
                     /locus_tag="PL056_11735"
     CDS             922..1347
                     /locus_tag="PL056_11735"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPM47732.1"
                     /translation="MKYILLVLLTVFSFSCDNFNRDLFSSEQNDDMPDMNDESAIPDE
                     NTIPDETQPPDETNDPDQIIETPDTFVRPDENETPDESANVVYEVTSSCRWCGRCAIA
                     CSVNAISNVGGKAVIDPEKCTGCGDCVSRCPRGAIRKKE"
     gene            1350..1835
                     /locus_tag="PL056_11740"
     CDS             1350..1835
                     /locus_tag="PL056_11740"
                     /inference="COORDINATES: protein motif:HMM:NF026048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPM47733.1"
                     /translation="MKKSSIVILILALFLGAGVFAAVKSTYKVIPEKCIGCKLCVDSC
                     PTKAIAMKNNKAVIDPEKCVNCGICAGKCPVKAIEPPKDKKQALDEEDKPEHQTKKVY
                     IADTRKCTGCKECVPTCPVKAISVSYGKVSIDPEKCINCGLCANSCAFNALKPSDIKI
                     K"
     gene            1832..2098
                     /locus_tag="PL056_11745"
     CDS             1832..2098
                     /locus_tag="PL056_11745"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPM47734.1"
                     /translation="MSRIFLTVLILLLFTGSCGIDPSEDSNITVDETLCIGSTCRICA
                     KTCKYDAVSFYGPESKALIDPLKCISCGDCISQCPTGAISGTVK"
     gene            2095..3300
                     /locus_tag="PL056_11750"
     CDS             2095..3300
                     /locus_tag="PL056_11750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47735.1"
                     /translation="MKKILIFLFLIPLFSFADSGFITFGGYGSFLKKTDSGLFSPSYF
                     FIDGYADISLADDLNFTAELQSVAGIPADQMRFLYNSYMFFSPRLQIAYSFKKVKIGL
                     ISTFNVYDQSENNSVPIYQNGMTAGGTRKFFTQHEFFLRAEPVKDLKISADLAYNIQM
                     FHPDQEIIKNDFRDSNIRAAGSISYSVFSLLSPFISVEHFNDLNKDNRFDLTGIKAGL
                     RGEKVFTGSGRFYYDLHYKRFESTSINDKNRMTLDLGYRWSNNASFDIFSGFYQEYSL
                     SENNDLHYVTRQLYAMFRYWPVKNKLALWTGAFVRFEELKKDFWMPVAPFAGVLCRVK
                     SFDAQARYIVRLDRDLQKKYSDINHRLDLSLIYTYRWFSPGISFLYNYRDEFSINSMG
                     INIYLSALM"
     gene            complement(3374..>4078)
                     /locus_tag="PL056_11755"
     CDS             complement(3374..>4078)
                     /locus_tag="PL056_11755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47736.1"
                     /translation="MYTKIGEWDKWKELTYKKDTLYGLDRRAGFGNMTGQYIVYYDYD
                     LEVQFCDLELGDAGCFRVSRAGEEARYPYFFNDKNTIYYFSRDMSDTSNASIIKADIT
                     DRDNIKYSVILKRNNVPGAWINNIDDRFILFTEKRITDGKEGKALQCYYRFSDKKVFC
                     MDGEFDLNFEKRVAYAYKRMYIYGAETDLNAVSRVLAVRDMECYCDYNPDKCPFEDYT
                     PNPENPKDPWKRPFKK"
BASE COUNT         1222 a          810 c          836 g         1210 t
ORIGIN      
        1 acggaaggga agtgcaaagg atatcagaca gttgcagata catccgaaat gatggaaatt
       61 cctgcgggaa aatactatgt cggaagctat tcaccccctg ccgacctcat tgatcccgat
      121 gaaagaccgt gggagagctg gatagcctac tatgaaaatt atcttccgct ctattacatt
      181 gataaatatg aagtgaccca gaaggattac agtgaatgtg ttgaagcggg aatatgttct
      241 gaaccggtaa agaaaagatc cggctggaga tcagactact ggggtaactc cgaatatgac
      301 aattatcctg ttttatatgt gaactattat gaagctgaaa aatactgcaa atggcgaggc
      361 aaacggcttc ccacagaatt tgaatgggta aaagcggcaa acggcggaga aatgagatgg
      421 tatccctggg gtgatgttga ccctgacagt gacaacagat tcgggaacat attctattca
      481 ctgaatgaga caaaaagtta cgatacagcc gaagtgggaa catatccgtt agatatatca
      541 ccattcggag ttatggactt aaacggcaat gtttcagaat ggacggcaag caactggaca
      601 aggatgctcg gctatgaagc gacatacggc gacaaactca aagtagtcaa aggcggcaga
      661 tgggaggaaa actccaatta cagcggatgg atagctcaga gaatggccgc caccccctgg
      721 acaagctcca ataccgtcgg attcagatgt gtgaaggatt aaaaattaat ttcaattagt
      781 tatgtcgttt ttcaaaggca actaaatagt atcataatat ggctctctta tttatttcag
      841 tttaatattt aaaaaaactg gaactttttc tatttttatg gactcttatg tccgtaattg
      901 atttttttaa tgcaggggct tatgaaatat attctgcttg ttctgttaac tgtcttttct
      961 ttctcttgtg ataatttcaa cagagatctt ttttcaagcg aacagaatga tgatatgccc
     1021 gatatgaacg atgagtccgc aattcctgat gaaaatacca ttcctgatga aacccagccg
     1081 cccgatgaaa caaacgatcc cgatcagata atcgaaacgc ctgatacatt tgttcgtcct
     1141 gatgaaaatg aaaccccgga tgaatcggct aatgttgtgt atgaagtgac gtcgagctgc
     1201 cgctggtgcg gaaggtgtgc aattgcctgt tctgtcaacg cgataagcaa tgtcggcggc
     1261 aaggcggtca ttgatcctga aaaatgtact ggctgcggag actgtgtatc aagatgcccc
     1321 agaggtgcga ttaggaaaaa ggagtaaaca tgaaaaaaag ttctattgtc atcctgatcc
     1381 ttgctttgtt tttaggcgca ggggtttttg cggccgtcaa atcaacatat aaagttattc
     1441 ctgaaaaatg tatcggttgt aaattgtgtg ttgacagctg tcccacaaag gcgatcgcaa
     1501 tgaaaaacaa caaagcggtc atcgatccgg aaaaatgtgt gaactgcgga atatgtgcag
     1561 gaaaatgtcc tgtgaaagcg atagagccgc ctaaagataa aaagcaggca cttgatgaag
     1621 aggataagcc ggaacaccag acaaaaaagg tttatattgc cgacacaaga aaatgtaccg
     1681 ggtgcaagga gtgtgttccc acctgtcccg ttaaggcgat aagtgtttct tacggcaagg
     1741 tctcgataga tccggaaaaa tgtatcaact gcggattgtg tgccaattca tgtgcattca
     1801 atgctctgaa accttcagat atcaaaataa aatgagccgg atattcctga cagttttaat
     1861 actgctcctt tttacgggat catgcggtat tgacccttct gaagactcta acattacagt
     1921 tgatgaaact ctctgcatcg gatcaacttg cagaatttgt gcaaaaacat gcaaatatga
     1981 cgctgtgagt ttttacgggc ctgaaagcaa agcactgatc gatcccctga aatgcatttc
     2041 ctgcggagat tgtatctccc agtgtcctac aggtgcaatt tccggaactg taaaatgaag
     2101 aaaatactga tatttctgtt tttaatccct ttattctctt ttgctgacag cgggtttata
     2161 acttttggcg gatacgggtc attcctgaaa aaaacagatt ccggtctttt cagtccgtca
     2221 tattttttca ttgacggata tgctgatata tcacttgccg atgatctgaa ttttactgct
     2281 gaacttcaga gtgttgcagg gattcccgca gatcagatga gattccttta taattcatac
     2341 atgttctttt ctcccagact gcagatcgca tattctttta aaaaagtgaa aatcggtctc
     2401 atcagcacat ttaatgtcta cgatcagtct gaaaataaca gtgttccgat ctatcagaac
     2461 ggaatgactg cgggaggaac cagaaaattt ttcacccagc acgaattttt tttaagagcc
     2521 gagccggtaa aagatcttaa aatttcagca gatctcgcat ataacattca aatgttccac
     2581 cccgatcagg aaatcattaa aaatgacttc agagattcaa atatcagagc ggctggatct
     2641 atttcatact ctgttttttc tcttctttcc ccgttcatct cagttgaaca ttttaacgat
     2701 ctgaataagg ataaccgttt tgatctaacc ggaataaaag cgggtctgag aggggaaaag
     2761 gtttttacag gatcggggag attttattac gatcttcatt ataaacggtt tgagagcaca
     2821 agcataaatg acaaaaacag aatgactctt gatcttggat accgctggag caacaacgca
     2881 tcttttgata ttttcagcgg attttatcag gaatattctc tgtcagaaaa caatgatctt
     2941 cattatgtta cccgtcagtt gtacgccatg ttcagatatt ggccggttaa gaacaaactt
     3001 gctttatgga caggtgcttt tgtgaggttt gaagaactga aaaaagattt ctggatgccg
     3061 gtggcgcctt ttgcaggagt tctctgccgt gttaaaagtt ttgatgctca ggcacgctac
     3121 attgtccgtc ttgaccgcga tctgcagaaa aaatattctg atataaacca cagactcgat
     3181 ctttctttga tatatactta ccggtggttc tctccgggaa tcagttttct gtataactac
     3241 agagatgaat tctcaataaa ttcaatgggg ataaatatct atctgtctgc tttgatgtga
     3301 ccgctttttt tctgatcttt gagcacaatt acaaacaaaa ctattatgat atatcttttc
     3361 atcgtttatt tactcacttc ttaaacggtc ttttccaagg gtctttcggg ttttcaggat
     3421 tgggtgtgta atcttcaaaa ggacacttgt cagggttgta atcacagtaa cattccatat
     3481 ctctgactgc caatactctt gaaacagcat taagatctgt ctcggcacca taaatataca
     3541 tcctcttata ggcatatgcg accctttttt caaaattaag atcaaactct ccatccatgc
     3601 agaatacctt tttatccgag aatctataat aacactgaag tgccttgcct tccttgccgt
     3661 cagtaattct tttttcggtg aaaagaataa atctgtcatc tatattattt atccacgcac
     3721 cggggacatt atttctctta agtataactg aatatttaat attgtccctg tcagtgatgt
     3781 ccgctttaat tattgaagca tttgatgtat ctgacatatc tctggaaaaa taataaatcg
     3841 tattcttatc attgaaaaag taaggatatc tcgcctcttc accggccctg ctcactctga
     3901 aacatcctgc atctccaagt tcaagatcac agaactgaac ttcaagatca taatcgtaat
     3961 aaactatata ctgaccggtc atatttccga aaccggctct cctgtccaag ccgtatagag
     4021 tatccttttt atatgtcagt tccttccatt tgtcccattc cccgatcttt gtgtacat
//
LOCUS       DAOOYB010000259         4071 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_26037_length_4071_cov_5.985558, whole genome shotgun sequence.
ACCESSION   DAOOYB010000259 DAOOYB010000000
VERSION     DAOOYB010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4071
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26037_length_4071_cov_5.985558"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..921)
                     /locus_tag="PL056_11760"
     CDS             complement(<1..921)
                     /locus_tag="PL056_11760"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPM47737.1"
                     /translation="MKTLELEITDLCNEKCLHCYHPEHKDKGFIKDLEKLERMLTEFG
                     DLGFIHVVITGGEPLLHPDFKAICTIAQKHRYLISVKTNGMLVDDDVTEFFQELKPKT
                     VEFSLYSADKDEHDKITQVAGSFDSTLRGIRLLNEHGVKISVMTPVLKGIKRWKELFK
                     LMKEMNVPWGCSQNIHSSFDERAEVENFKGTTDSYFDFLQFVNGLENQEIKNIDDLCF
                     KECEGGISTVCVAPDYSVRACIPFPDSAGIYTGGNCEELLKKARKQLEERFAKLECHS
                     CELVKYCNPCPAHALHVGRAWIAVFYKFTAM"
     gene            complement(918..1469)
                     /locus_tag="PL056_11765"
     CDS             complement(918..1469)
                     /locus_tag="PL056_11765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47738.1"
                     /translation="MDPAIRSRRESVLRSLYSSENGLIVHCSAVEDSGSSFIFAGVAD
                     AGKSTLANKLSKIMTVINDDMNVLEFSDAGIKVSTYFTQAENQGYHYIINENVSGLLK
                     AVIFPVKEKETESFLEPLSDKGLIWKMLLNCVAPPITGEDHLFSNYYKIIDRLIESVP
                     FFNIHHHLKDSSEFIAELLRSVK"
     gene            complement(1469..1609)
                     /locus_tag="PL056_11770"
     CDS             complement(1469..1609)
                     /locus_tag="PL056_11770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47739.1"
                     /translation="MEKNYLKPEIINETKLERTLVYAKAQTPEDECTTLQGKIDQDTK
                     LS"
     gene            complement(1611..>4071)
                     /locus_tag="PL056_11775"
     CDS             complement(1611..>4071)
                     /locus_tag="PL056_11775"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HPM47740.1"
                     /translation="NHDNQSSTTEIDINAVVSDPSITLKKSYNHALGAGPVIQGVSVE
                     ASAWMVPSDVDCTGDNCDTEIEVWTDAPAGSTAYVSINGGAEVSKTTVSNSGQSYADF
                     DITLDNSIAYNTIEVRLVSTTLAESDSTDYIKIDETLPQLTLINPATCTGICRRKDLS
                     DDPVTTDLIELAELGYGYDDDAVAGGVLNFKTAGAIQFTVEGATGGTVAIETVSGISN
                     GTASISYDSIGGYYYADFTTLTATDSNADGQTDYNLVFKVTESPSGAVSRYLVKLHVD
                     LKKPAAVDLSGKVSTYKKEGRVEMNWDAVAGNNSIFGDLPGAVYRYDVRYQDYNTASC
                     TIGTTFTSAKKPIETIAGPVPDPVLAGEMGYEFFVNRLDNGKLTTDPLYREFDTHKNG
                     NKYCFAAAAVDAVYASDGTVLAENLGNIAPNDTGEMKMEWTQIRTDCAGSHYAVLRDL
                     GDLDGDSLNDFTIADKFKSSDCVNNDLAGNVKIVFSKGEDSFEVIGSAANDRLGIGVS
                     SKANFNGDEYLDFAYADKYGKVFVHYGTVDGLSTTPSFQFTAKDNSTQNFRTMAVGDY
                     SGDGCDDIAVSAPGAGGSGVARGQVYVYFGRGESCDSDVAIDGNSPDVTFEGGSDNDE
                     LGRGEIYAGADMNGDGKTDFILPSYKNVYIAYGGDSGGQIVTYTITGLKDLPGLRIGS
                     GNLNGDGYSDVVIGDESRILIHYGSSTGISGTASVTINDISTVNYNYPPSVTNFALAI
                     SHNIPDINGDGAGDLITASSRGLLGYFTNNDVIMTMPSIYDPFVSTTSVNLKILMLEY
                     GIVYCDSLSAKGSCYILSYGE"
BASE COUNT         1160 a          916 c          745 g         1250 t
ORIGIN      
        1 catagctgtg aacttgtaaa atactgcaat ccatgccctg cccacatgaa gagcgtgggc
       61 agggcatgga ttgcagtatt ttacaagttc acagctatga cattcaagct ttgcaaaccg
      121 ctcttcaagt tgttttcttg cctttttcag cagttcttca caattaccgc ctgtataaat
      181 tcctgcagaa tcgggaaatg gaatgcaagc cctgacagag taatcaggag caacacaaac
      241 agttgagatc ccgccttcgc attctttaaa acaaagatcg tcgatgtttt taatttcctg
      301 attttcaagt ccgttcacaa actgaagaaa atcgaagtaa ctatccgttg ttcctttaaa
      361 attctcaact tcagctctct catcaaatga agagtgaata ttttgagaac aaccccacgg
      421 aacattcatc tctttcatca atttaaaaag ctctttccag cgctttatcc cttttaaaac
      481 cggtgtcatg acagaaatct taacaccgtg ttcatttaat aacctgattc ctcttaaagt
      541 tgagtcaaaa ctgccggcaa cttgtgtgat cttatcgtgt tcatctttat cagcggaata
      601 gagtgaaaac tccacagttt tcggcttaag ttcttgaaaa aactcagtaa catcatcatc
      661 aacaagcatt ccgttggttt ttaccgaaat gagataacgg tgcttttgag caattgtgca
      721 tattgcttta aaatcgggat gaagcaacgg ttctccgcct gttatgacaa catgtatgaa
      781 tccgagatct ccgaattctg taagcattct ctcaagcttt tcaagatctt ttataaaccc
      841 tttatcttta tgctcaggat ggtaacagtg aagacatttt tcattgcaaa gatcagttat
      901 ttcaagttca agagttttca tttaactgac cttaaaagtt ctgcgatgaa ttcagaactg
      961 tctttcaaat gatgatggat gttaaaaaat ggaactgatt caattaatct atctattatt
     1021 ttgtaataat ttgagaagag gtgatcttcg ccggttatcg gtggagcaac gcagtttaag
     1081 agcattttcc agataagacc tttgtcactc aacggctcaa gaaatgactc ggtttctttc
     1141 tctttcacag gaaaaattac ggcttttaac agacctgaga cattttcatt gataatataa
     1201 tgatatccct gattttccgc ctgagtgaag tatgttgaaa cttttatgcc ggcatcagaa
     1261 aattcaagaa cattcatatc atcatttatc actgtcatta tcttgctcag tttgtttgcc
     1321 agcgttgatt ttccggcatc ggcaacccct gcgaaaatga atgaactgcc agaatcttca
     1381 acggctgaac aatgtacaat gagcccgttt tctgatgaat aaagagaacg gagaaccgat
     1441 tccctccttg atcttattgc aggatccatt aagagagctt ggtgtcctga tctatctttc
     1501 cttgaagagt tgtgcattcg tcttccggag tctgtgcctt tgcataaaca agagttcttt
     1561 caagtttagt ttcattaatg atttcaggtt tcaaataatt tttttccatt ttattctcca
     1621 taactaagta tataacaact tccttttgct gataaactgt cacaatatac gattccgtac
     1681 tcaagcatta atattttaag atttaccgat gttgtgctta caaaaggatc atagatcgaa
     1741 ggcattgtca taataacatc attatttgta aagtatccca gtaaccccct gcttgatgcc
     1801 gtaataaggt cacctgctcc gtcaccgttg atgtcaggta tattgtgact gattgccaat
     1861 gcaaaatttg ttactgacgg aggatagttg taatttaccg tagaaatatc atttatcgta
     1921 actgacgctg ttccggaaat tccagttgaa cttccatagt gaataagaat tctactctca
     1981 tcacctatta caacatctga atatccgtca ccattcaaat taccgctgcc aattctgagt
     2041 ccaggaagat ctttgagtcc ggttattgtg taagtcacta tctgtccgcc agaatcacca
     2101 ccgtaagcga tataaacatt tttataactt ggaagaataa aatccgtttt gccgtcaccg
     2161 ttcatatctg cgccggcata tatttcacct cttccgagtt catcgttgtc agaaccccct
     2221 tcaaaagtaa catcgggaga attaccatct attgcaacat cagagtcgca actctcacct
     2281 cttccaaaat aaacatatac ctgtcctctt gcaacacctg aacctccggc accaggagct
     2341 gaaaccgcta tatcgtcaca accgtcaccg ctgtagtccc caactgccat tgttctgaaa
     2401 ttctgtgtag aattgtcttt tgcagtaaac tggaatgaag gagtggtgga aagaccatca
     2461 acagtaccat aatgtacaaa aacctttcca tatttatcag catatgcaaa atcaagatat
     2521 tcatccccgt taaagtttgc cttgctactt actccgattc caagacggtc gtttgcagcg
     2581 gaaccgataa cttcaaatga atcttcacct tttgagaaaa ctatttttac atttccggca
     2641 agatcgttat tcacacagtc actgctttta aacttatcag caatggtaaa atcattcaaa
     2701 ctgtcaccgt caagatctcc gagatctcga agaactgcat aatgtgatcc tgcacaatca
     2761 gtcctgatct gagtccattc cattttcatt tcgccggtgt cgttcggagc gatgttcccg
     2821 aggttttcag ctaacactgt tccgtcactt gcatagactg catcaactgc ggcagcagca
     2881 aaacagtatt tgttgccgtt tttatgcgta tcaaactccc tgtaaagagg atctgttgtc
     2941 agctttccat tgtcaagacg gtttacaaag aattcatatc ccatttctcc cgcaagaaca
     3001 ggatccggaa caggcccggc aattgtctcg atcggttttt tcgcacttgt gaaagttgtt
     3061 ccgatcgtgc aggaagcagt gttataatcc tgatatctca catcatatct gtaaacagca
     3121 ccgggaagat cgccgaatat tgaattattg cctgcaacag catcccagtt catctcaacg
     3181 cgaccttcct ttttataggt cgaaaccttc cctgaaaggt caacagcggc cggtttctta
     3241 agatcgacat gaagtttcac aagatacctg ctcactgcac ctgacggaga ttccgtaact
     3301 ttaaaaacaa ggttatagtc ggtctgcccg tctgcattgg aatcggttgc agtaagagtg
     3361 gtgaaatcgg catagtaata tccgccgata ctgtcgtagg aaatcgaagc tgtaccgttt
     3421 gagatccctg aaactgtctc tattgcaaca gttccgcctg ttgctccctc aactgtgaat
     3481 tgtattgcac ctgccgtttt aaaattaagc actccgcctg caacagcatc gtcatcataa
     3541 ccgtaaccga gctctgcaag ttcgatcaga tcggtggtaa caggatcatc agaaagatcc
     3601 tttcttctgc aaattcctgt acaggttgcc ggatttatta aagtaagctg cggcagagtt
     3661 tcatcgatct ttatataatc cgttgaatcg ctttcagcca gagttgttga aaccagcctt
     3721 acttcaattg tgttgtaagc gattgaatta tccagagtaa tatcaaaatc tgcataggac
     3781 tgaccggaat ttgaaacagt ggtttttgaa acttcagctc ctccgtttat tgaaacataa
     3841 gccgttgaac ctgccggagc atctgtccat acttcaattt cagtatcaca gttatctcct
     3901 gtgcaatcca catcagaagg aaccatccac gcagatgctt caaccgaaac accctgaatc
     3961 acaggacctg ctccgagagc atggttatag ctctttttga gtgtgataga cgggtctgat
     4021 acaactgcat tgatatcgat ctcagttgtt gatgactggt tatcgtgatt a
//
LOCUS       DAOOYB010000260         4069 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_26051_length_4069_cov_13.720229, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000260 DAOOYB010000000
VERSION     DAOOYB010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4069
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26051_length_4069_cov_13.720229"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..1831)
                     /locus_tag="PL056_11780"
     CDS             complement(35..1831)
                     /locus_tag="PL056_11780"
                     /inference="COORDINATES: protein motif:HMM:NF015148.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT family oligopeptide transporter"
                     /protein_id="HPM47741.1"
                     /translation="MSDEKLKINSHEEDDRLWLENVYQGDVKQLTLRAIIMGAIIGGF
                     MSLTNLYIGLKTGWGLGVTITACVLSFSIWKVLRKLFPWFVKTDMTLLENNSMKSTAS
                     SAGYTTGGTMVSAIAAYILITGKHLDFWVLTGWTIFLGIAGVVMAIPMKKQMINMEKL
                     PFPSGTATAETLKSLYAGEESRKQTNSLLGFALIGVVLTVVRDLFEWIEYTYAVFGEK
                     LAKYTVSMETSLIMVAAGGIMGWRTAWSILFGAILNYLVLAPIMFDMGAIKELGYRGI
                     NSWSLWFGASVMVSAGFTSFFMQWKTAMKAFSGVKDIFSKEKRELTAIEKVEVPMSWF
                     FWGIMIGGAGIAFIMWYSFGISIWMSILAIILSFFLAIVACRATGETDTTPVGALGQV
                     TQAFYGMIAPGSMVTTLMTASVTSGIAGSSADLLTDLKTGYLLGANSRKMFLAQFIGI
                     FAGTAVVVPAFYLIVPTPDVLGSDYFPAPAAQVWSGVAKLLAQGIASLHYTAKIAMIV
                     GVLVGIIIPVIDKLFPKASRYIPSSMGIGLAFVIPFWNSLSMFIGALIVLFIEKKRAA
                     LTEYVVPAASGIIAGESLAGVFIILVMTLMGK"
     gene            complement(1821..3923)
                     /locus_tag="PL056_11785"
     CDS             complement(1821..3923)
                     /locus_tag="PL056_11785"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HPM47742.1"
                     /translation="MKKIFYFLILLSLISGCSDEKKEDIYPDYEQKSDDDDNDSFDPA
                     DNENPVPDNDSDDPVNEGEEDTDPVDENNENENEEIPDWENDICTPNPCVMENSDGIC
                     TPFKDSFKCGCLEDHYWINGTCAEDPCIDDPCDTVENSTGQCLVDGKGYLCVCADNFY
                     WDKDSCKPVPGIIFVKADAKGKNDGTSWVDAFTDLTDAVESAGEPSSTQWIWVAKGTY
                     KPKKEMNAYECGGQRCINFAMRNNVSIICGFSGTESKIGERDFTKNETVLSGDLDNDG
                     TLSDGDSYHVFFNFNIDETSVIDGCIIEGGNADYGDSGIKDVHMKFGGGINNYNEVTA
                     IIRNVVFRNNYGFIGGGMANMNNSAPRIENCTFENNSSVRGGGIGNSMSSPKIVSSTF
                     SGNFTVYENTGFGGAMFNNEGSSPEIEDSLFENNTSVDGGAMVNLDMSHPSITGTIFR
                     NNTASRNGGAISNGYQSDPDIVNSLFIDNRSEMGGGAIEIYQESHPRIVNSKFLFNRV
                     PEKDFSFGGAISVNTSSIDIINSLFAGNSANSGGAVTAQWTIMNIVNSTITLNAARGE
                     YSYGGGVYSTGTDGETYIINSIIYDNYSDTSGNEISNDLTLTEVSYSNIKGCIAFGSW
                     DNDCGDDKEWNIDLDPMFHDINLGDFSLSADSPCIDKGTNDPFETDGIAENVLKDLLG
                     NDRIINQTADMGAYENVR"
BASE COUNT         1087 a          941 c          823 g         1218 t
ORIGIN      
        1 aacagccggt tctcaaattt tgtggtcttt ttttctattt tcccatcagc gtcatgacaa
       61 ggattatgaa tactccggca agactttctc cggcgattat tcctgatgcg gcggggacca
      121 catattcagt gagtgccgcc cttttctttt caatgaagag gacaatgaga gctccgatga
      181 acattgaaag ggagttccag aaagggatta caaatgcaag tccgattccc attgaagaag
      241 gaatgtatct tgaagctttg ggaaaaagtt tatctatcac cggaattatt atgccgacaa
      301 gaactccgac gatcattgca attttagctg tatagtgaag agaagctatt ccctgagcga
      361 ggagttttgc aactcctgac cagacctgtg cggcaggagc agggaaataa tcgcttccga
      421 gcacatcagg tgtcggaacg ataagataaa aagcgggaac aacaactgcg gttcctgcaa
      481 agatcccgat gaactgcgca aggaacatct ttcttgaatt tgctcccaga agatatccgg
      541 ttttaagatc tgtcagaagg tctgctgaag agccggctat acctgaagtt actgaggcgg
      601 tcataagcgt tgtcaccata ctgccgggag cgatcattcc gtaaaatgcc tgagtaacct
      661 gaccgagtgc tccgaccgga gttgtgtcgg tttcacccgt tgcacggcat gcgacaatgg
      721 caagaaagaa agaaaggatt atggcaagaa tgctcatcca gatggaaatt ccgaatgaat
      781 accacatgat gaatgcaatt ccggccccgc ctatcataat tccccagaag aaccagctca
      841 taggcacttc aactttttca attgctgtaa gctcccgttt ttcttttgaa aaaatgtctt
      901 ttacgcctga aaatgccttc atcgcagttt tccactgcat gaaaaacgag gtgaatccgg
      961 ccgaaaccat gacagaagca ccgaaccata agctccaact gttgatccct ctgtatccga
     1021 gctctttaat cgcacccatg tcaaacatga tcggggctag aacgaggtaa ttcagtatcg
     1081 caccgaaaag gatgctccat gccgttctcc agcccattat tccgccggct gcaaccatta
     1141 ttaagcttgt ctccatcgaa acagtgtatt ttgccagttt ttcaccgaaa acagcgtaag
     1201 tgtactctat ccactcaaaa aggtcgcgga caacagtgag cacgacccct atcagagcaa
     1261 aaccgagcaa gctgttggtc tgttttctgc tctcttcacc ggcatataaa cttttaagtg
     1321 tttcagctgt tgcagttccg gaagggaagg ggagcttttc catgtttatc atctgttttt
     1381 tcatcggtat cgccataact actccggcaa tgcccagaaa tatcgtccat ccggtaagta
     1441 cccagaaatc aagatgtttt ccggttatca gaatataagc cgcaattgca ctcaccattg
     1501 ttccgccggt tgtatagcca gccgaagaag ctgttgattt cattgaatta ttttcaagaa
     1561 gcgtcatgtc ggtctttaca aaccacggga aaagttttct aagtactttc cagatgctga
     1621 atgaaagaac acatgcagta attgtcacgc cgagtcccca gccggtcttc aacccgatgt
     1681 aaagatttgt caaactcatg aatccgccga ttatggcgcc catgattatg gctctgagcg
     1741 taagctgttt cacatcaccc tgataaacat tttcaagcca taatctgtca tcttcttcgt
     1801 gactgttgat ctttaatttt tcatctgaca ttttcgtaag ctcccatatc tgcggtttgg
     1861 tttattatcc tgtcgtttcc aagaaggtct ttcagcacat tttcagcaat tccatctgtt
     1921 tcaaaagggt cgtttgtccc tttgtcaatg catggtgaat cggcagaaag agaaaaatcg
     1981 ccaagattga tgtcatgaaa catcgggtca aggtcgatgt tccattcctt gtcgtctccg
     2041 caatcattat cccatgaacc gaaagcgata catcctttta tatttgaata cgaaacttcg
     2101 gtcaaagtga gatcgtttga tatttcattt ccggaagtgt ctgaataatt gtcataaatt
     2161 attgaattta ttatataagt ttctccgtca gttccagtgc tgtaaactcc tccgccatag
     2221 ctgtattccc ctcttgcggc attaagagtt attgtgctgt tgacaatgtt cataatagtc
     2281 cactgtgctg ttactgctcc gcctgaatta gcagaatttc cggcaaaaag ggagttgatg
     2341 atgtcgatcg aactggtgtt gacagatatt gcgccgccga aactgaaatc tttttccgga
     2401 actctgttga aaaggaattt tgaatttact attcttgggt gactttcctg atagatctct
     2461 atcgctccgc cacccatttc agaacggttg tcaatgaaaa gagagttgac aatgtcagga
     2521 tcgctctgat aaccgttgct gatcgctccg ccgttccttg atgcagtatt gttcctgaag
     2581 atcgttcccg ttattgaggg atggctcatg tcaagattta ccattgctcc tccgtcaaca
     2641 gaggtgttgt tctcaaaaag tgaatcttca atttcgggac tgctcccttc attattgaac
     2701 atcgctccgc cgaaacctgt gttctcataa acagtaaaat tgcctgaaaa agttgatgaa
     2761 acgattttcg gagagctcat ggagtttccg attccgccac cgcggacaga gctgttattc
     2821 tcaaatgtac agttttcgat tcttggggct gaattgttca tgttggccat tccaccgcct
     2881 atgaatccgt aattattgcg gaaaacgaca tttcttataa tagctgtcac ttcgttataa
     2941 ttgtttatgc cgccaccgaa tttcatgtgg acatccttaa tgccgctgtc gccgtagtca
     3001 gcattccccc cttcaattat acagccgtcg atgactgaag tttcgtcgat attgaaatta
     3061 aagaagacat gatagctgtc gccgtcactt aaagttccgt cgttatcgag atctccgctt
     3121 aaaacagttt cattttttgt gaaatcccgt tccccgattt ttgattctgt tcctgaaaag
     3181 ccacagatta ttgaaacatt attcctcatt gcaaagttga tgcatctttg tccgccacat
     3241 tcataggcat tcatctcctt tttcggttta tatgttcctt ttgccaccca tatccattga
     3301 gttgaagatg gctctccggc actttcaacg gcatctgtga gatctgtgaa agcgtcaacc
     3361 cacgaagttc cgtcattttt cccttttgcg tccgccttta caaagataat tccgggaaca
     3421 ggtttgcacg aatccttgtc ccagtaaaag ttatcggcac agacgcagag ataacccttt
     3481 ccgtcaacga ggcattgtcc ggtcgaattt tcaactgtat cgcaaggatc gtcaatgcac
     3541 ggatcctcag cacatgttcc attgatccag taatgatctt caagacatcc gcatttgaat
     3601 gaatctttga acggagtgca gatcccatct gaattttcca tcacacaggg attcggagtg
     3661 cagatatcat tttcccagtc ggggatctcc tcattttcgt tttcattatt ttcatcaacg
     3721 ggatctgtgt cttcttcccc ttcattgacc ggatcgtcgg aatcgttgtc aggaacaggg
     3781 ttttcgttat cagcagggtc aaagctgtca ttatcgtcat catcagattt ctgttcataa
     3841 tcagggtaaa tgtcctcttt tttttcgtcc gaacaccctg aaatcagact taaaaggatt
     3901 agaaaataaa aaatcttttt cattcaaacc tcaaaaactc gaaaaatatt cctgaacata
     3961 ttaactcatt acagtttaaa taaaatattt tttactcatt cgggaataaa tcagacattt
     4021 attttgttac ttataacggt tggtcactgc cgttcggcaa gttcagaaa
//
LOCUS       DAOOYB010000261         4066 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_26078_length_4066_cov_6.749688, whole genome shotgun sequence.
ACCESSION   DAOOYB010000261 DAOOYB010000000
VERSION     DAOOYB010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26078_length_4066_cov_6.749688"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>4066
                     /locus_tag="PL056_11790"
     CDS             <1..>4066
                     /locus_tag="PL056_11790"
                     /inference="COORDINATES: protein motif:HMM:NF019298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HPM47743.1"
                     /translation="SHSETVTSLTSEIVMELGKDYVPGLEELSNLLMKYSIKWSDYDL
                     SGKLSMFRISPRIKTQILGSAQIIKGEESTFRLFVNDVLSGDPIMSGDPIMSKDLNVE
                     LFSGEQLVGTFTAKTDEFGMASVPVSVDEFVFGTLKMVVTTETAYGTRSVEAEVVAEQ
                     LSKILLTTDKPLYQPGQTIHIRTLSLELPAKEPIADSDITLEITDPKGNKVYKEKGKT
                     SGYGIFAADFTLANLVTLGNYTISAVVDGSISSEKTITVERYVLPKFGIDFKADKGFY
                     MPGETVKGSITSNYFYGKPVSGGSVHIEVKTFDVSENTVKIIDATLTKDGKYNFEAKL
                     PDYFTGSELDQGKATVKFEITVTDTANHEQSTVKTIKVVKSPVIATLVPAAGKMLPGI
                     TQDMYLVLTDPNGTPLAAEVTVKAGENSEKITVGESGFAEFKMVLTDTEAVKVTVKYE
                     DKTFESDVVFTKDGNSEFVFLSTDSSIYGTGDTMTVNIFTGFDPDMPQPSMLPDRAYI
                     DVIADGQIRLMQTVEFKEGKGSTDITLDETMHGPVEILAYYLTKEGNIIRSSKGVYVR
                     KASSLNIEFTTDKEEYKPRETAKAQFMVKDTEGKPVVAALGVAMVDEAVFHVMDFIPG
                     MEQTYFNIESSVMESTYAIYGTSYEEIVTPVEDEEQAEEIEKKADAFFAGNAGNLSHG
                     VAADDYSEPENAYLSASTTAAKAKADAIFKALDNGETSDCTDSAITQEKLDEILAKQE
                     NTDPWDNIMSGTPGAASYEKAVATLISAGPDETAGTKDDITMEIKVCEASWNGMEDGT
                     GGDPTEGDTGNSGDTGDTGDSGDSDYGDTGKGVKVREWFPETLYYNPQLITDEKGVAE
                     LEVLMADSITTWRVTSLANSMNGGLGSSLDSIRVFQDFFVDIDFPVFLTQNDEVAVPV
                     GIYNYLNESQKITLEADTEEWFEMTGASAIEVTVPANSVSAAYFPIKVLKIGKHDLTV
                     FAHGSKMSDAVKRSVTVKPDGIMADKSESALLNGNKSITITIPENAIADSAELFVKIY
                     PGIMSQAVEGLDSMFQMPYGCFEQTSSATYPNILVLQYMIATDTLTPEIELKARDFIT
                     QGYQRLLTYEVTGGGFEWFGAEPAHFVLTAYGLLEFVDMSAVHEVDPAIITRTAAWMA
                     SKQNSDGSFIPTSGGIAEGAINNFENSLLRTTAYGAWALSRADKEETARKNAVKYLED
                     NASKAEDTYSMAMTAIALVKNGGSSTVINKLISDILDKKIEDEEGGAHWEQTVNTETY
                     SSGDGANLETTALIGLLLLDKGGYSDVVGQILNWLVRQKDSFGNWSTTQGTILALRFM
                     IESLNAGTSEADATVKVSANGSPETTIVITPENS"
BASE COUNT         1278 a          833 c          952 g         1003 t
ORIGIN      
        1 gatcgcattc tgaaacggta acttcgctga ccagtgaaat agtcatggaa cttgggaaag
       61 attatgttcc gggacttgag gaactttcca atctgttaat gaaatattca ataaaatgga
      121 gcgattacga tctgagtggc aaactttcga tgtttcgaat atcccccaga ataaaaaccc
      181 agatacttgg aagtgctcag ataattaagg gcgaagaatc aactttccgt ctttttgtta
      241 atgatgttct ttcgggagat ccgataatga gcggcgatcc gatcatgagc aaagacctca
      301 atgttgaact tttcagcgga gagcagcttg tcggaacatt cactgcaaaa actgatgaat
      361 tcggaatggc atccgtccct gtttctgttg atgaatttgt tttcggcact cttaaaatgg
      421 tggtgacgac cgagactgca tacggaacaa gaagcgttga agctgaagtc gttgcggaac
      481 aactgagcaa aatacttctg actactgaca aacctctcta tcagcccggt cagacaattc
      541 acatccgcac cctttcactt gaacttcctg caaaggaacc gatagcagac agcgatataa
      601 ctcttgaaat aaccgatccg aaaggcaaca aggtctataa agagaaagga aaaacaagcg
      661 gatacggaat atttgccgct gactttactc ttgcaaatct tgtaactctc ggaaattaca
      721 caatttccgc tgtagttgac ggcagcatct cttctgaaaa gaccataact gtcgaaagat
      781 atgtccttcc taagttcggg atagatttca aagccgacaa gggattctac atgccgggtg
      841 aaactgtaaa gggctctata acttcaaatt atttctatgg aaagccggtg agcggaggaa
      901 gtgtccatat cgaagtaaaa actttcgatg tatctgaaaa tactgtgaaa ataattgacg
      961 caactctgac taaagatgga aaatataatt ttgaagcaaa acttcctgac tattttacag
     1021 gaagcgagct cgatcagggc aaggcaaccg taaaattcga gataactgtc acagacacag
     1081 caaaccacga acaatccaca gttaaaacaa taaaagtggt gaaatcacct gtgatcgcga
     1141 cacttgttcc cgccgccgga aaaatgcttc cgggaattac tcaggatatg tatctcgtac
     1201 tcaccgatcc gaacggaact ccgctcgcgg ctgaagtcac tgtaaaagca ggtgaaaatt
     1261 ctgaaaagat cactgtcggt gaaagcggtt ttgcagagtt taaaatggtg ctcaccgata
     1321 ccgaagctgt aaaagttaca gtaaaatacg aagacaagac atttgaaagc gatgtggttt
     1381 tcaccaaaga cggcaacagc gaatttgtat tcctttcgac agattcgtct atttatggca
     1441 ccggagatac gatgaccgtc aacattttca caggttttga ccctgatatg cctcagccgt
     1501 caatgcttcc cgacagggca tatattgatg taatagccga cggacagata cggctgatgc
     1561 agacagtcga attcaaggaa ggcaaaggga gcacagacat cactcttgat gaaactatgc
     1621 acggacctgt tgagatcctt gcttattacc ttacaaaaga aggaaatata atccgttctt
     1681 caaaaggtgt ctatgttaga aaagcaagct ctctcaatat tgaatttaca accgataaag
     1741 aagaatataa accaagggaa acggcaaaag cgcagtttat ggtgaaagat actgaaggga
     1801 aaccggtcgt tgcggcactc ggagttgcaa tggttgatga agctgttttt catgtgatgg
     1861 atttcattcc cggaatggag cagacttatt tcaacattga aagttcagtg atggaatcga
     1921 cttatgcgat ctacggaaca agttatgaag agatcgtcac acctgttgaa gatgaagaac
     1981 aggcagaaga aatagaaaag aaagccgatg cgttctttgc aggaaatgcg ggcaatttaa
     2041 gccacggagt tgcggctgat gattattctg agccggagaa tgcatatctt tcagcttcta
     2101 ccactgcggc aaaagcaaaa gctgatgcca tttttaaagc tcttgataac ggtgaaacct
     2161 ctgactgcac tgattcagcg ataactcagg aaaaacttga tgaaatactt gcaaaacagg
     2221 aaaacaccga cccgtgggat aacataatgt cgggaactcc cggagcggca agctatgaga
     2281 aagccgttgc aacattgatc tcggcagggc cagatgagac cgcaggaaca aaagatgata
     2341 taacaatgga aataaaggtt tgtgaagcaa gctggaatgg aatggaggac ggcacaggag
     2401 gagatccgac tgaaggagat accggtaata gtggagatac aggtgataca ggcgacagtg
     2461 gtgattcaga ttacggtgac acaggaaaag gggtcaaggt gcgtgaatgg ttccctgaaa
     2521 cgctttacta taatcctcaa cttattactg atgagaaagg tgtcgctgaa cttgaagttc
     2581 tcatggcaga ctcaattacc acatggagag ttacatctct tgcaaacagc atgaacggcg
     2641 ggcttggcag ctctcttgac agcatacgcg ttttccagga tttcttcgtt gacattgatt
     2701 tccctgtttt ccttacgcaa aacgatgaag ttgctgttcc tgtcggaatt tacaattacc
     2761 ttaatgaatc tcaaaagatc actcttgagg cggatactga ggaatggttt gagatgacag
     2821 gcgcatccgc aattgaagtg acagttcccg caaattcggt ttcagccgca tatttcccga
     2881 taaaagttct taaaatagga aaacacgacc ttacagtatt cgcacacggt tcaaaaatgt
     2941 cagacgctgt gaaaagaagt gtcaccgtta aacccgacgg cataatggct gataaatcgg
     3001 aaagtgcact gctgaacggc aacaagagca taaccatcac aattcctgaa aatgcgattg
     3061 ctgacagtgc tgaacttttc gttaagatct atccaggcat catgtctcag gcagttgaag
     3121 ggcttgattc gatgttccag atgccatacg gctgttttga acagacttca tcagctacat
     3181 atccgaacat tcttgtcctt cagtatatga tcgcaacgga tactctgacc cctgagatag
     3241 agcttaaagc aagggatttc ataactcagg gttatcagcg gcttctcact tatgaagtca
     3301 ctggcggcgg atttgaatgg ttcggtgctg agccggcaca ttttgttctc actgcttacg
     3361 gacttcttga atttgttgac atgagcgcag tccacgaggt cgatcctgcc attatcacaa
     3421 gaactgcggc atggatggcg tcaaaacaga actctgacgg gtcatttatt cctactagcg
     3481 gcggaattgc tgaaggggcg ataaataatt ttgagaattc gctccttaga actacagctt
     3541 acggtgcctg ggcactttca agggctgata aagaagaaac tgcaagaaag aatgcagtaa
     3601 aataccttga ggacaatgct tcaaaagctg aagacacata ttcaatggca atgaccgcga
     3661 ttgcacttgt taaaaatggt ggaagttcaa ctgtcataaa taagcttatc agcgacattc
     3721 ttgacaagaa aatcgaagat gaagaagggg gcgcacactg ggaacagacc gtaaatacag
     3781 aaacctacag tagtggcgat ggtgcaaatc ttgaaacgac cgcgttgatt ggacttctcc
     3841 ttcttgataa aggagggtac agtgatgttg tgggccagat actcaactgg cttgtaaggc
     3901 agaaagattc tttcggcaac tggtcaacga ctcaggggac gatccttgct ttgagattca
     3961 tgatcgaatc gcttaatgcc ggaacttctg aagctgatgc gactgtaaaa gtgagcgcaa
     4021 acggttcacc tgaaacgaca atagtcatca ctcctgaaaa ctccga
//
LOCUS       DAOOYB010000262         4059 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_26145_length_4059_cov_10.272228, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000262 DAOOYB010000000
VERSION     DAOOYB010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4059
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26145_length_4059_cov_10.272228"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(148..1194)
                     /locus_tag="PL056_11795"
     CDS             complement(148..1194)
                     /locus_tag="PL056_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010182720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HPM47744.1"
                     /translation="MNRKEMSIEADSRQFFNEIKCLIEKSRQQVAVAVNSAMSLLYWQ
                     IGKRINEKVLNNKRAEYGGQIVSTLSRQLKTEYGNGWSEKQLRHCLRFAEIFPNNEIV
                     SALRRQLSWTHIKTLIYIEDELKREFYIEMCKIERWSTRTLQDRINSMFYERTAISKK
                     PEEIIRNDLELLKNEKQLTPDMVFRDPYFLDFLGLSDKYSEKDLESAILVELQHFIIE
                     LGSDFAFLARQKRITVDNDDYYIDLLFYHRQLKCLVAIDLKLGKFEAAHKGQMELYLR
                     WLEKYETVEGESLPLGLILCASKNEEHVELLQLCKSNIRVAEYLTKLPDRKLLEKKLH
                     QAIERAKSKLVSND"
     gene            complement(1776..3719)
                     /locus_tag="PL056_11800"
     CDS             complement(1776..3719)
                     /locus_tag="PL056_11800"
                     /inference="COORDINATES: protein motif:HMM:NF017539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NACHT domain-containing protein"
                     /protein_id="HPM47745.1"
                     /translation="MDASEILKNVGNNMGAVVIIWKILHEKYKKYAEKRQLDAARYLP
                     ARDHYIEYLSRTYGNLSIVNTLYSKNISYKTKDIYEPLEVFWHDPFGKKIKHTISNDK
                     PFSLLSKFNKLVIQGAAGSGKSVVLKMLFLDIVDRSMGVPVYIELRRLSSSHKIFDEF
                     LSQITPDGTSLNKEILWDCIGDGDFIFLFDGYDEIQPSQISQVNDDLKDFCSRCSKNK
                     FVITSRPRPGLNINGFNTVFFENIDYDQCCRIIKKYDSQGAICEKLIKEVKDFKFLEG
                     ENLLLSPLMAYFLYVFSLKGKSLPSKKHVFFRTFFDMLFSDHDELKGCPKTFKSLLSR
                     DQFHSVLRELGFISFKEQKIEFSKEEILLFIRRSLDKLGYSEISESDVLGDLFNDIPI
                     FVSNDDFYFKWSHRSIQEYFAACYIFRCEYGEKASVLRSCFFSNPQSHLDMIDIYCDM
                     DPVVFRETFVYEYLKKYRQYHFMRPDDIPAELEQEVSFRKEILFVWKLFLSFFITEGG
                     LFNPDNFYYFCLDCSRRFKVKIYGATGRYVSIGGFDSMLLISRSFDNLYHVTDFLFKR
                     GFSWVEKISSDDPFPASLSMFPPSEIERLSDLPDLTATINNSQNFADINKVVKFGMHG
                     LYKINSRAVFSEIINIENSLKTK"
BASE COUNT         1333 a          782 c          621 g         1323 t
ORIGIN      
        1 actgaggtga aaaatgaaac aatttcttct tgactttaaa gggaataact gacccctaag
       61 aaagagatcc agatcacatt taagtagatt ttctaaatag tttttgagat ttatatatat
      121 ttattgagca ctaatccctg agaaatttta atcgtttgac accaatttgc tttttgctcg
      181 ttcaattgcc tgatgaagct tcttctcaag caattttctg tccggcagtt ttgttaaata
      241 ttcagcaact ctgatattac ttttacatag ctgaagaagt tcaacatgct cttcgttttt
      301 agaagcacac aaaataagac caagaggaag actttcgcct tcaacagtct catatttttc
      361 aagccatcta aggtaaagct ccatctgtcc cttgtgtgcc gcctcaaatt ttccaagctt
      421 taaatcaatt gccaccagac attttaattg tctgtgataa aaaagaaggt caatataata
      481 atcatcatta tcgaccgtta tccttttctg ccgtgccaga aaagcaaaat ctgagccaag
      541 ctctataata aagtgttgta attcgacaag aattgcactt tccagatctt tttcggaata
      601 tttatcactg agtcctaaaa aatcgagaaa atatggatct ctaaaaacca tatcaggcgt
      661 taactgcttc tcattcttta gaagttccaa atcatttctt attatttctt cgggtttttt
      721 gctgattgca gtgcgctcgt aaaacattga attaatacga tcttgcaaag tgcgggtact
      781 ccatcgctct attttacaca tttctatata gaattcccgt ttcaattcat cttcaatata
      841 gatcaaagtt tttatatgcg tccagctcaa ttgtctccgc agtgcagaga caatttcatt
      901 attaggaaaa atctccgcaa agcggagaca atgtctcagt tgtttttcgc tccagccatt
      961 gccatattca gtttttaatt gtctcgacag tgtagagaca atctgtccac catattctgc
     1021 acgtttattg tttaatactt tttcatttat tctttttcca atctgccagt aaagcagact
     1081 catagctgag tttactgcca cagcgacctg ttgacggctc ttttcaatca agcactttat
     1141 ttcattaaag aactgtcttg aatctgcttc tatcgacatc tctttcctgt tcatcatttt
     1201 cctcttttca tttctcaaat tctaaactga acataataac ggcaaagatt acacctctgc
     1261 attttaaaaa tcaagccaac taattgaatt tactatagat aattcgctta agagataatt
     1321 gtatgatttt atgctttcgt aggggttgga caccgtttca accctgtttt tttcgtttcc
     1381 gtcgtagggg cgaacttgtc ctgtcttcga ctctgagggg tgagccctca ggctcagact
     1441 cgaacgagag ttaatcgaag gatcacgccc ttttctttgg ttttggattt gatttctatt
     1501 ttatcacatg aacatcacat aaaaaagcat acgatttcaa gatattatat cccaaaagtt
     1561 aggttttatc acatgaccgt cacatgaggt taaaaaaaat gaattacaca ccggttacat
     1621 aagactaagc agagataaaa atttaacagt caaacaaatt cgctacttgt cctgagtcaa
     1681 tcgaaggatg gtgataatta tgtaattgtg ctgacctcta ataatttaga ggagatgaaa
     1741 aatcaaggaa caataatctc gataagttat cgaattcact tcgtttttaa tgaattttca
     1801 atatttataa tttcactgaa cactgcacgg gaattaatct tataaagacc gtgcattcca
     1861 aacttaacaa ccttgttaat atcagcaaaa ttctgtgaat tatttatcgt ggctgtaaga
     1921 tcgggtaaat ctgacaatct ttctatttca gaagggggaa acatgcttaa agaagctgga
     1981 aaaggatcgt cgcttgaaat tttttcaacc catgaaaatc ctctcttgaa gagaaaatcc
     2041 gtaacatgat acaaattatc aaaagatctg gatataagga gcatcgaatc aaaaccgcca
     2101 atactaacat atctaccagt cgcaccatat attttgactt taaatcttct agaacaatct
     2161 aaacaaaaat aatagaagtt gtccgggttg aaaagacctc cttcggttat aaaaaaactt
     2221 aaaaaaagtt tccagacaaa aaggatctct tttctaaaag acacctcctg ctccagttca
     2281 gcaggaatgt catcgggtcg cataaagtgg tactgcctat attttttaag atattcataa
     2341 acaaaagtct ctctgaacac tacaggatcc atatcacagt aaatatctat catgtctagg
     2401 tgggactgag ggttactgaa aaaacaactc cgcaatacag atgctttctc accatattca
     2461 caacgaaata tataacaagc cgcaaaatat tcctgaatag agcgatgtga ccacttaaag
     2521 taaaaatcat cgtttgatac aaatatggga atgtcattaa ataaatcacc cagaacatct
     2581 gattctgaaa tttcagaata acccagttta tccagagatc ttctgataaa taacagaatc
     2641 tcttctttgg aaaattcaat tttctgctct ttaaacgaaa taaacccgag ttcccgcaat
     2701 acagaatgaa actgatccct cgaaagcaaa gatttgaatg ttttaggaca acccttcaat
     2761 tcatcatgat ccgaaaaaag catgtcaaaa aaggttctaa agaaaacatg ttttttactc
     2821 gggagtgatt ttccttttaa actaaaaaca tacagaaaat aagccattaa aggggacagc
     2881 aacaaatttt ccccttcgag aaatttgaaa tctttaactt cttttattaa tttttcacag
     2941 attgcccctt ggctatcata ttttttgata attctgcaac actgatcata atctatattc
     3001 tcaaaaaaaa ccgtgttaaa tccattaatg ttaaggcctg gtcttggtct tgaagtaata
     3061 acaaacttgt ttttagagca tcttgagcag aaatccttta aatcatcatt aacttgagaa
     3121 atctgagatg gctgtatttc gtcatatcca tcaaaaagaa aaataaagtc accatcacca
     3181 atacaatccc acagaatttc tttattaaga gaggtaccgt ctggcgttat ctgacttaaa
     3241 aactcatcaa atattttatg gcttgaagac aatcgtctaa gttcaatata aacaggaaca
     3301 cccatagatc tatcaactat gtcgagaaac agcattttaa ggacaactga ttttccagat
     3361 cctgccgctc cctgtataac aagtttatta aatttgctca atagagaaaa cggtttatca
     3421 ttagaaatag tgtgttttat ttttttccca aaaggatcat gccagaaaac ttcaagaggc
     3481 tcatatatgt cctttgtttt gtaagatata tttttagaat aaagagtatt tacaatggaa
     3541 agatttccat aagtgcgact caaatattca atgtaatgat ctctagcagg aagatacctc
     3601 gccgcatcca actgcctctt ctctgcatac tttttgtatt tttcatgtaa aattttccaa
     3661 ataataacaa cagctcccat attatttccc acatttttta aaatttcaga agcatccata
     3721 tttcttagcc caaaccctca acgattttta tttcatcttc tggagtccgt aaagttcata
     3781 aacttttttg ttatcgttgc tgttgtctaa tagcattttt tcctctcttc ttcaacaaga
     3841 atgcagataa tataatcaaa ctgaatcgaa tagtaaataa aagttgactg gtgattatta
     3901 atgtttccct gtgttaccct cttccctttt ttgacatact ttttcatggg ggcataatac
     3961 ttttgcggaa tgaaaaattt accttagcga atagggagtc agtgatggaa actaaaatgg
     4021 tgatgatcgt gtcagcattc ccttcaaaag aggtggcaa
//
LOCUS       DAOOYB010000263         4056 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_26169_length_4056_cov_5.715821, whole genome shotgun sequence.
ACCESSION   DAOOYB010000263 DAOOYB010000000
VERSION     DAOOYB010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4056
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26169_length_4056_cov_5.715821"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="PL056_11805"
     CDS             complement(<1..228)
                     /locus_tag="PL056_11805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47746.1"
                     /translation="MIRNKQIFLGFLIFFTFLSVWADEENVITVREINKKEKKDYKYY
                     LIYSVGIGYGKIWYQYIDGQTDDIVEVAEGKM"
     gene            complement(215..1699)
                     /locus_tag="PL056_11810"
     CDS             complement(215..1699)
                     /locus_tag="PL056_11810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47747.1"
                     /translation="MRILKIMSKFLVFVAVFLFSSCGTTKMLTAGFENGKAKVNVKET
                     QIDTVLIERRTMSLVRNVNNNTYYFANSNSVAVGDLSVIWFEKDGQKEIKLETVSKEH
                     KKTLFISSFDLIPDKKIIFAGRVVKKTDKEKAVHTFVAEYDWTGKLISEKVMVLNNEE
                     KSEWVNAIRIVEDGFLIAADYVEEEIEGGFWSGTYDFLVKNSLFAKLNTKGDVLWNQM
                     VGANQVSDSISGFDMVKDGYIIYGTTEGDLIQKHSGGQCVSWQRYNPDAGIYVPNFYQ
                     CNDLFIMKFDFDGNKVWASQIGKEGRKNFEESLTKFHRKEVKFRWDVSAINDLVEYTL
                     KNHSFGFNNSKLNNDLNQIFVWGYEGQKALLYSFSTMDGSLIKKDEIVYEGYNSEETK
                     KDKFDKMFTRINNIIYNQKEGVLINGIISELYGVYTTWPKYFFFMPLSKKISESVLLH
                     GIYMYPPFFINENESSITKFGYYGNFEYILPENAELTEEKFDKK"
     gene            1899..2507
                     /locus_tag="PL056_11815"
     CDS             1899..2507
                     /locus_tag="PL056_11815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47748.1"
                     /translation="MKKITLIAVVIFALLFAVACSMVKYKKGDQTKGYLSGIKKIAVQ
                     YDYSTARVGKFATEGEYVDSKVEEHNKKEAGKGDEWKNKWEEQKTATFATKFESLINE
                     YTKEKGIEVGSNIADAELTMSVILTYIEPGWNIGISRQDASISMRIEIFKTGEAGEPL
                     VVYEINNVPGRGAMGYDFDAGYRMGEAFALAGKELGKFLVKE"
     gene            complement(2533..3780)
                     /locus_tag="PL056_11820"
     CDS             complement(2533..3780)
                     /locus_tag="PL056_11820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47749.1"
                     /translation="MKKMFLFAGVFVTFLIFNGCGESVTKNNSNVNCFTEGAYQCTVD
                     LLQLCVNSAWFTVKDCSESGKICEEFDNDETVTAACVDPETGDTGNTGDTGDSGDSGN
                     SGNSGDSGNSGDSGNSGDSGNSGDSGDSGDSGNSGDSGNSGDSGNSGDSGNTGTCTLS
                     DSWYAPDPSWTAYAYLKMTGEIGDYQGDYVEAVSVKGRTKTVSGTKDFTANGSFMSYQ
                     GQILLADAVDYEFINQTSTSATVDYYDVMWQFSPQIIPLLKEDGINESDFGAFVWFRH
                     SFIDLTIQNSSVTSQRTRKSCWLGISATEEVEEEGDTYDVPIGGMYGCFDYNVDGSVG
                     ETLKMMFKNQMTDDRDDILTFINTGDDGSVLQYGDEEFQFECQCYNEDGASDYEDNEV
                     PCWEYDGPGGAEDCPPEIEGGCE"
BASE COUNT         1269 a          855 c          581 g         1351 t
ORIGIN      
        1 cattttacct tcggctactt cgacaatatc atcagtttgt ccgtcaatat actgatacca
       61 tattttcccg tatccgatac cgacagaata tatgagatag tatttgtaat cttttttttc
      121 ttttttattt atttcacgaa cagttataac attttcttcg tcagcccaaa ctgataaaaa
      181 tgtgaaaaaa atcaaaaatc caagaaaaat ctgtttattt cttatcaaac ttttcctccg
      241 ttaattctgc gttttcagga agaatatact caaaattacc gtagtatcca aattttgtga
      301 tgctcgactc attttcatta ataaaaaatg gtgggtacat atatattcca tgcaagagaa
      361 cagattcaga tattttttta ctcaaaggca taaaaaagaa atatttcggc catgtggtat
      421 acactccata aagctctgat attattccat taattaaaac accttctttc tgattataaa
      481 taatattatt tattcttgta aacattttat caaatttatc ttttttggtt tcttcagaat
      541 tatatccttc ataaactatc tcgtcttttt taataagaga cccgtccatt gttgaaaaac
      601 tgtataataa agctttttgt ccttcatagc cccagacaaa tatttgattg agatcattgt
      661 ttaatttgct gttgttgaat ccaaaagaat gatttttcag agtatactct accaaatcgt
      721 taatcgcact cacatcccat ctaaatttta cttctttacg atgaaatttt gtaagacttt
      781 cttcaaagtt ttttcttcct tctttaccaa tttgacttgc ccaaactttg ttgccatcga
      841 aatcaaattt cataataaat aaatcgttac actgataaaa attaggaaca tatatacctg
      901 catccggatt ataccgttgc caagaaacac actgcccgcc agagtgtttt tgaattaaat
      961 caccttcagt tgttccataa ataatatatc catctttaac catatcaaat cctgaaatag
     1021 aatcactgac ttgatttgca ccaaccattt gattccacaa cacatctccc tttgtattaa
     1081 gctttgcaaa caaagaattt tttaccagaa aatcgtatgt accactccaa aaaccgcctt
     1141 caatctcttc ttccacatag tctgctgcaa ttaaaaaacc gtcttcgact attcttattg
     1201 cattcaccca ttcacttttt tcctcattat ttagtaccat aactttttct gaaattaatt
     1261 tgccagtcca atcatactct gcaacaaaag tatgaacagc tttttcttta tcggtttttt
     1321 taacaactct accggcaaaa attattttct tgtcaggaat taaatcaaat gaggaaatga
     1381 aaagtgtttt tttatgttct tttgaaacag tttccagttt tatctctttt tgcccatctt
     1441 tttcaaacca gatcacagaa agatctccca cagcaacgct gtttgaattc gcaaaatagt
     1501 atgtgttgtt atttacattt ctcactaacg acattgttct tctttctatt aaaaccgtat
     1561 caatttgagt ttctttcaca tttacctttg cttttccatt ttcaaaacct gctgtcaaca
     1621 tctttgttgt tccacaggaa gaaaacaaaa aaactgcaac aaaaactaaa aatttactca
     1681 tgatttttaa aattctcatt ttttcacctc acttgatttt aaaattttta ccactccgca
     1741 caaaaaattc tgttgtttat atcctacaag ctgtgttttt taatgtcaaa tttttgtagg
     1801 atattgccta cagctatttt ttgtaaaaac ataatacttc atctctgaaa aagtgctttc
     1861 gggtaatatt aaaaaattgt tttttgtgag gtaaaaaaat gaaaaagatc acgcttatcg
     1921 cagttgttat ttttgcactg ctttttgcag ttgcatgttc catggttaag tacaaaaaag
     1981 gtgaccagac aaaaggatac ctgagcggaa tcaaaaaaat cgctgttcag tatgattatt
     2041 caacagcaag agtcggcaag ttcgcaacag aaggcgaata tgttgacagc aaagttgaag
     2101 agcacaacaa gaaggaagca ggaaaaggtg acgaatggaa aaacaaatgg gaagaacaga
     2161 aaacagcaac tttcgcaact aagtttgaat ctcttatcaa tgaatataca aaagaaaaag
     2221 ggattgaagt cggttcaaac attgcagatg ctgaacttac aatgtcagtt attcttacat
     2281 acatagaacc gggctggaat atcggtattt caagacagga tgcaagcatc agcatgagaa
     2341 ttgaaatatt caagaccggt gaagcaggcg aaccgcttgt tgtttatgag atcaacaatg
     2401 tcccgggtcg tggtgcaatg ggttatgatt ttgacgccgg ataccgtatg ggcgaagctt
     2461 tcgcacttgc aggaaaagaa ctcggaaaat tcctcgttaa agaataattc tactgacttt
     2521 caataaaaat ccttattcac atccgccttc aatctcagga ggacaatctt ctgctccgcc
     2581 gggaccgtca tattcccagc atggaacttc attatcttca taatcagaag ctccatcttc
     2641 gttgtagcac tgacattcaa actggaattc ttcatctcca tactgaagaa ctgagccgtc
     2701 atcaccagtg ttgatgaatg taagtatatc gtctctgtca tctgtcatct gatttttaaa
     2761 catcattttc agcgtttcgc cgaccgatcc gtcaacattg taatcaaaac agccatacat
     2821 tccgccgatc ggaacatcat atgtatcccc ttcctcttca acttcttctg ttgcagaaat
     2881 tcctaaccag caacttttcc ttgttctttg acttgttacc gaactattct gaatggttag
     2941 atctataaat gaatggcgga accacacaaa tgccccgaaa tcagattcat taattccgtc
     3001 ttcttttaaa agaggaatta tctgaggact gaactgccac attacatcat agtaatctac
     3061 tgtggctgaa gttgatgttt gattaataaa ctcataatct actgcatcag caagaagaat
     3121 ctgaccctga tagctcataa atgaaccgtt tgcagtgaag tcttttgtac ctgaaactgt
     3181 tttcgttctt ccttttactg atactgcttc aacatagtca ccctggtaat ctcctatttc
     3241 acctgtcatt ttcagatatg cgtatgctgt ccagcttgga tcaggagcat accacgaatc
     3301 tgaaagtgtg catgttcctg tgtttcctga atcacctgaa tttccggaat ctccactgtt
     3361 tcctgagtca cccgaatttc cagaatctcc tgaatcgccc gaatctcctg aattacctga
     3421 atctcctgaa ttacctgaat ctcctgaatt acccgaatct cctgaattac ctgagtttcc
     3481 tgaatcaccc gaatctcctg tatctccggt gtttcctgta tcaccggttt ctggatcaac
     3541 gcacgctgcg gtaacagttt catcgttatc aaattcttca cagatctttc cgctttcact
     3601 acaatccttc actgtaaacc aggcgctgtt cacacaaagc tgaaggaggt caacagtgca
     3661 ctgatacgcc ccttcagtaa aacagtttac attagaattg tttttcgtga cactttcacc
     3721 acaaccgtta aaaattaaaa aagttacaaa tactcctgca aataaaaaca tctttttcat
     3781 aattacaccc ctcttaaaaa aatcttgttg caccggaata ttactataaa cttaaagata
     3841 catcaataaa attattatta cagcctgttt aatttctgtc aacccaaaac attcctcgtt
     3901 ttagtaaaca cccaaaactt tgcttcctta aaaaacaata tcatttttca aatcacaact
     3961 tagaacaact tttcattgtg ccaagtgatc ctgaaatgtc gcccatgtct ttatattctg
     4021 tcggatgcca aagatcgata taccctgtta tggtta
//
LOCUS       DAOOYB010000264         4023 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_26472_length_4023_cov_11.413558, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000264 DAOOYB010000000
VERSION     DAOOYB010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4023
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26472_length_4023_cov_11.413558"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="PL056_11825"
     CDS             <1..663
                     /locus_tag="PL056_11825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47750.1"
                     /translation="ADYNALAQCVETNCASDQTIECYLEKCESESNSCGIKKATAYGT
                     ASFNVNMNEIGHQADAQDDTRGIMQSAFATGTYGSSNLSFEPAGVAAMQSSVTYIATP
                     QQGDPYLDVTQVPVFDQGGQAAGGNPVVIVSFDPTLLAVGTIEMGPYQGMGAFGAVVD
                     VNWSTQQVTCYHGFFEGNVNVTQVGDITAHGAFAFTGNVDLIYTGDYNFSGTPPTACT
                     KN"
     gene            772..2898
                     /locus_tag="PL056_11830"
     CDS             772..2898
                     /locus_tag="PL056_11830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47751.1"
                     /translation="MLLKRCLNLIVIASLLFTFSIISSCTSKPEISEKFSGNLTGLVV
                     GMPRPGGNGGLTEVRDTLSTDETNHAVRGFWNPVKNKYDAEFVWFDPEGKKAYTKIMN
                     MSPDWKNTTIHYKGNGPLKAGTWTIAVNVDGKYLGKTEFSVVRDRSKIPLVAQIEAFN
                     NEKLTLNEAHFIAEKIKCYADGICDENNVTSDIPETLSKRNVGLAVSVFRKGELTDVA
                     FSSSSKLENAMKEIVKSVKVNNANPASIEISVVHSAINIKPSEQAVSLKMRQGMGFSI
                     SKDGKIATMLSTQIARRQIADATELLRQLSIDAGMAENGWQESKLTAFMTQDFVLAAG
                     MDETKEIAFSRTVVPVDSVTRSDLVKSVDSAFEWYMKNQLKNGRYMYTFFPSKDLEPD
                     DDWALRNLNGLFVLAEIAKDRKDERMIASVKKAIELFRSGLKLENGYKYLDWKEHRAV
                     SSIAGTAFLLGAMTELYEPSYKEDMKLMADAIISLQEENGKLKTDFYRPLRDIDQMYY
                     PGETLLVLIRYYKHSKYEPALKTVEKAFPYYREFWNKKENQDGPFVPWQIRAYQETYM
                     VTKDKKHAEFVFTLMDWMLKKYTPLGRDAVPGRQGALDTQFASTAVYSEGLSQAYALA
                     LELKDENRIKYYGKILKGTLGYLLGLQFKPEDTYWIKRPEKVIGATALRPDINELRLD
                     ATYHAISAIHYTTKLLDENSWNAIIW"
     gene            2915..>4023
                     /locus_tag="PL056_11835"
     CDS             2915..>4023
                     /locus_tag="PL056_11835"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HPM47752.1"
                     /translation="MFQLFNFSELNSRVLRFAKYLETFSIDCAVLTSNQDIFYFTGSV
                     QKGTLVIKKEGECVYFVRKNLLRAQVESPQCVKSWDSDGIAAMIKGIWSLPLDVTTVS
                     EYLFYKKKFALEKDPADCSLPLSLTKMIKSDSELELMKKAGIMNQSVMEHAKKVFRQG
                     DRDIDIQAEIEKFAKKELGHQGLFWVRDANMEASMGLVVTGDSALAPTYTDFPIGGIG
                     LSPAVAQGASGEVIKNSFVIDFVGCQYGYCADSTRTFFIGEPAEKIKKTYEELNEFLY
                     NVKQLLVPGKTGEEIYAFALKELEKYSWKANFMGNEQKVKFIGHGIGTEVNQLPVVAE
                     KQKIPLENNMVIALEPKVFLPGYGIIGIENTFVIK"
BASE COUNT         1257 a          792 c          903 g         1071 t
ORIGIN      
        1 gctgattaca atgctcttgc acagtgtgtt gaaaccaatt gtgctagtga ccagacaatt
       61 gaatgttatc ttgaaaaatg tgaatctgaa tctaatagct gcggtataaa aaaagctact
      121 gcttatggaa cagccagttt caatgtcaat atgaatgaaa tcggtcatca agccgatgct
      181 caggatgaca caagaggaat aatgcagagt gctttcgcca caggaactta tggctcatca
      241 aacttaagtt tcgagcctgc cggcgttgca gcaatgcagt caagcgttac ttatattgct
      301 acacctcagc agggcgaccc ttatcttgat gttacacagg ttcccgtttt cgatcagggc
      361 ggacaggctg ctggcggaaa tcctgttgta atagtcagtt ttgatccgac tcttcttgct
      421 gtcggcacaa ttgaaatggg accatatcag ggaatgggtg ctttcggtgc tgttgttgat
      481 gttaactggt caacacaaca ggtaacttgt tatcacggat tttttgaggg taatgtcaat
      541 gttactcaag ttggagatat aacagctcac ggtgcgtttg cttttactgg aaatgttgat
      601 ctcatttata ccggtgatta taatttcagt ggcacacccc cgactgcctg cacaaaaaat
      661 tagttttttc acaccccggg ttcgtcccgg ggtttttttt atccaaaaat tgtcattttt
      721 ttcattttat gtaacatctg gcttgttttt taatattttt gtgaggttat catgctttta
      781 aagagatgtc tgaacctgat cgtcattgct tctcttttgt tcactttttc cattatttca
      841 tcctgtacat caaaaccgga gatttctgaa aaattttcag ggaatcttac aggacttgtt
      901 gtaggaatgc cgagaccggg agggaacggc ggattgacag aagtgcgcga cacgctctct
      961 acagatgaga caaatcacgc tgtaagaggt ttctggaatc ctgttaaaaa caaatatgat
     1021 gcggagttcg tctggtttga tccggaaggg aaaaaagcat atacaaaaat aatgaacatg
     1081 tctcctgact ggaagaacac aacaattcac tataaaggaa acggcccgct taaagcagga
     1141 acatggacaa tagcagtaaa tgttgacggc aaatatcttg gaaagacaga gttttccgtt
     1201 gtcagagacc gttcaaagat acctcttgtc gctcagatag aagcattcaa caatgaaaag
     1261 ctcacactca atgaagcaca cttcatcgca gagaaaatta aatgttatgc cgatggaatt
     1321 tgtgatgaga acaatgtcac atctgacatc cctgaaacac tttcaaaaag aaatgtcggg
     1381 cttgcggttt cagttttcag aaaaggggaa ttgaccgatg tcgctttttc atcttcatca
     1441 aaacttgaaa atgcaatgaa agagatcgtg aagtcagtaa aagtaaacaa cgcaaaccct
     1501 gcttccatcg agatttcagt ggttcattcg gcaataaaca tcaaaccttc cgaacaggcg
     1561 gtatccctta aaatgagaca gggaatgggt ttttcaattt caaaggatgg aaagatcgca
     1621 acaatgcttt caacccagat cgcaagaaga cagatcgctg atgcgacaga acttttaaga
     1681 cagctttcaa ttgatgccgg aatggctgag aacggatggc aggaaagcaa gctgaccgcc
     1741 tttatgacac aggatttcgt tcttgccgca ggaatggatg aaacaaagga gatcgctttt
     1801 tccagaaccg ttgtacccgt tgattcagta acaaggagcg atttagtcaa aagcgtagat
     1861 tcggcatttg aatggtatat gaaaaatcag cttaagaacg gaagatatat gtatacattc
     1921 ttcccttcaa aagatcttga accggatgat gactgggctt tgagaaatct gaacggactt
     1981 ttcgtccttg ccgagatcgc taaagacaga aaagatgaaa gaatgatcgc aagcgtgaaa
     2041 aaggcgatag aacttttcag aagcggactt aaacttgaaa acggttacaa atatctcgac
     2101 tggaaagagc acagagccgt ttcaagcatt gcagggaccg cattccttct gggtgcgatg
     2161 acagagcttt acgagccctc atacaaagag gacatgaaac tcatggcaga tgcgataatt
     2221 tctcttcagg aggaaaacgg caaactgaaa accgatttct accgcccgtt aagagacatc
     2281 gaccagatgt actatccggg agagacactg cttgtgctga taagatacta taaacattca
     2341 aaatatgagc cggctttgaa aaccgttgaa aaagcgttcc cttattacag agagttctgg
     2401 aataaaaagg aaaatcagga tggacctttc gttccatggc agataagggc ttatcaggaa
     2461 acatatatgg tcacaaaaga taaaaaacat gccgagtttg tcttcacact tatggactgg
     2521 atgctcaaaa aatatactcc tctcggaaga gatgccgttc cgggcaggca gggcgctctt
     2581 gacactcagt tcgcttcaac ggccgtttat tcagaaggcc tttcccaggc atatgccctt
     2641 gcgcttgaac ttaaagatga aaacagaata aaatactacg gaaaaattct taaaggaact
     2701 ctcggatacc ttcttggact gcagtttaag ccggaagaca cttactggat caaacgacct
     2761 gaaaaagtga tcggtgcaac agctttaaga cccgatataa acgaactccg gctagatgcg
     2821 acatatcacg caatatccgc aattcattac acgactaagc tgcttgatga aaatagctgg
     2881 aacgcaataa tctggtagtt ttcaggagat gaccatgttt caacttttta atttttcaga
     2941 actgaattca agggtgttga gatttgccaa gtatttggaa acattcagca ttgactgtgc
     3001 agtccttact tccaatcagg acatttttta ttttaccggc tctgttcaga aaggaactct
     3061 tgtaattaaa aaagaggggg aatgtgtcta ttttgtaagg aaaaatctct taagggcgca
     3121 ggtggaatcg ccccaatgtg ttaaaagttg ggattcagac ggtatcgcag caatgataaa
     3181 agggatatgg tctcttcccc ttgatgttac caccgtttcc gaatatcttt tctacaagaa
     3241 aaaattcgcc ctcgaaaaag atccggctga ctgctcactt cccctttcgc tgacaaaaat
     3301 gataaaaagc gattccgagc ttgaattgat gaaaaaggcg gggatcatga atcaatctgt
     3361 catggaacat gcaaaaaagg ttttcagaca gggcgacaga gacatcgaca ttcaggcgga
     3421 gattgaaaaa ttcgcaaaaa aagaactcgg acatcagggg cttttctggg tgcgggatgc
     3481 gaatatggag gcctcgatgg gacttgtcgt gaccggagat tccgcacttg ctcctactta
     3541 taccgatttt ccgatcggcg gcattggtct ttcccctgct gtggctcagg gtgcgagcgg
     3601 cgaagtgata aaaaacagct ttgtgataga ttttgtcgga tgccaatatg gatactgtgc
     3661 agacagcact cgtacttttt tcatcggtga gccggcagaa aaaataaaga aaacatatga
     3721 ggaattaaac gaatttcttt ataatgttaa gcagttactt gtgccgggaa aaacggggga
     3781 agagatatat gcttttgctt taaaagaact tgaaaaatac tcctggaaag caaatttcat
     3841 gggaaatgaa cagaaagtga aattcatcgg tcacggcatc ggaactgaag taaatcaact
     3901 tcctgttgtt gccgaaaaac agaagatccc tcttgaaaac aacatggtca ttgcacttga
     3961 acccaaagtg tttctgcccg gatacggcat tatcggcata gaaaacactt ttgtaataaa
     4021 aga
//
LOCUS       DAOOYB010000265         3987 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_26815_length_3987_cov_4.242879, whole genome shotgun sequence.
ACCESSION   DAOOYB010000265 DAOOYB010000000
VERSION     DAOOYB010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3987
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26815_length_3987_cov_4.242879"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="PL056_11840"
     CDS             complement(<1..730)
                     /locus_tag="PL056_11840"
                     /inference="COORDINATES: protein motif:HMM:NF014589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase N-terminal
                     domain-containing protein"
                     /protein_id="HPM47753.1"
                     /translation="MIHSDWDSLKRVVNYFNDNEVTRKVLGLSEKDHLSMPGKLMGLA
                     SHGKKKQDILNELRNIENQKKIEGNILNSIIKKGLSIPEPTTYWGFKDIAATMQCDFE
                     DSVIDYIRKWKNATGCSHLYYSGGAALNIHTNSRIENELNFDSISIPPVPSDCGLALG
                     AAAVAEWNDGRKIKRTGPFIVNSLSEKNNKLKRISSRRIKEIAELISNGKIIGVCIGN
                     AETGPRALGHRSIIARPDKIDIRKK"
     gene            complement(737..1234)
                     /locus_tag="PL056_11845"
     CDS             complement(737..1234)
                     /locus_tag="PL056_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47754.1"
                     /translation="MLTIGIYGFSNTKDGFESHDNAIALIRNGKVLANLELERFSRVK
                     QDSNLTEHFDMLIKPWLKKDENLRFVLSTSFTGDTFRSKSGSIFIKVPENFKPGDIIA
                     PCEIKLSGFLKEHAATGWVVSHELAHIGSCLPFYGSFKENSLLIHIDGGASVSANSVW
                     TYTNG"
     gene            complement(1234..2574)
                     /locus_tag="PL056_11850"
     CDS             complement(1234..2574)
                     /locus_tag="PL056_11850"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HPM47755.1"
                     /translation="MKKEENKPILNHSENDYLCNGERCFNKTFKKAMSFHWPEGLAAV
                     SDETGAYHIKADGSPLYHERYDETFGFYEGIASVRKSGKNFHIDTAGVPVHDRSFIWS
                     GNFQVGCCVVRDVKGFFHIGKDGKDKYRERYEYAGDFRYGIAVVHRNGKAWHIFEDGT
                     QLHNKQFLSADVFHKGYAVVADDSGVYHIDKAGNPLYDHRFLSAEPFYNGAALCKTKK
                     GRIVRLFENGHFEHLPVIDPCIDIEEIRKRINGGEKVVMLLRHAARDKIPEGTWGMDV
                     DLNEKGHEMSEKLGRLFHGTGNWNYFSSPVQRCANTCLSFSKGQNNGTPDVVLSKLLG
                     EPGTFTDPDNKINWKPQEFGKIAEKYVHEGIHKGFRPLSVGCELIISLVENSINNSPI
                     LMITHDFFVAGLFKYLGLHHPELNDWVQYLDGVVFFSDGKKITEWRRFNGMEVV"
     gene            complement(2679..3491)
                     /locus_tag="PL056_11855"
     CDS             complement(2679..3491)
                     /locus_tag="PL056_11855"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPM47756.1"
                     /translation="MNEQQIRRSQIKLLNGRQLFDIELTTRCNKHCYCCPRQNLKRKN
                     RDMSISIFDKVCDWLPQNCDVIFAGFGEPLLHKDCALFVNKLSKKSVGVSILTNGKLL
                     TTEKTIELFDAGLERLQISILQRDETNEIAKYIEMTKNIDNIKIRFNIIYETTPMLPD
                     NIYADLLENNYEIFFKKIHNRANKLYKDRNSNEIITCGTFFIDSFIDTNGLLILCCND
                     INGNNSFGNVTEISFVELQNKKREFLGNTPISSICDCCTDEYRFKHLNFNDK"
     gene            complement(3787..>3987)
                     /locus_tag="PL056_11860"
     CDS             complement(3787..>3987)
                     /locus_tag="PL056_11860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47757.1"
                     /translation="PPNLWDHDGERISTLTECGNLEILKVEQHNNKLHITCSEKPFSF
                     IFFRPRQIETKVVVYGGEGIWG"
BASE COUNT         1090 a          824 c          690 g         1383 t
ORIGIN      
        1 tcttttttct aatatctatc ttatccggtc gtgcaatgat actcctgtgc ccgagtgctc
       61 tgggtcctgt ttcagcgttt ccaatgcaga ctccgatgat ttttccattg gaaataagtt
      121 ctgcaatttc tttaattctc cggctggata ttctcttcag tttattattc ttttccgaca
      181 aagaattcac aataaacggt cctgttctct ttattttcct gccatcgttc cattctgcaa
      241 cagctgctgc tcccagtgcc agtccgcagt cacttggaac aggcggaata gaaatactgt
      301 caaagttcag ttcattttca attctgctgt tagtgtgaat gtttaaggcg gcacctcctg
      361 aataataaag atgactgcaa cctgtagcgt ttttccattt tcttatgtaa tcaataacgc
      421 tgtcttcgaa atcacactgc attgttgccg caatatcttt aaacccccaa tatgtagtgg
      481 gttcagggat agaaaggccc tttttaataa ttgagtttaa tatgtttcct tcgatctttt
      541 tctggttttc aatgttgcga agttcattaa ggatatcctg cttttttttg ccatgactgg
      601 caagccccat aagttttccc ggcatagaaa ggtgatcttt ttcagaaagt ccgagaactt
      661 ttctggtgac ttcattgtca ttaaagtagt tgacaactct tttaagtgag tcccagtcag
      721 aatggatcat tgacagtcat ccgtttgtat aggtccagac agagttggca gatactgatg
      781 caccgccatc aatgtgtatt agaagcgaat tttccttaaa agacccgtaa aaagggagac
      841 agcttccgat atgtgccagc tcatgactta caacccagcc ggttgccgca tgctctttaa
      901 gaaatcctga aagttttatt tcacatggag cgatgatgtc acccggtttg aagttttccg
      961 gaacttttat aaagatactc ccggattttg accggaaagt gtctccagtg aatgatgtgg
     1021 agagaacaaa cctgaggttt tcatcttttt tgagccacgg tttaatcagc atgtcaaaat
     1081 gttctgtcag gttgctgtcc tgttttacac gactgaatct ttcaagctca aggtttgcca
     1141 atacttttcc gtttcttatc aaagctattg cgttgtcatg tgactcaaaa ccgtctttgg
     1201 tgttggaaaa tccatatata cctatggtca gcattaaacg acctccattc cgttaaatct
     1261 tcgccactca gttatttttt tgccatctga aaagaaaacc acaccatcca gatattgtac
     1321 ccagtcattt aattcagggt gatgcaatcc gagatattta aataacccgg caacaaaaaa
     1381 atcatgggtt atcataagta taggtgaatt gtttatggag ttttcgacga gcgatattat
     1441 cagctcacac ccgacagaga ggggtctgaa tcctttatga ataccctcgt ggacatattt
     1501 ttccgcaatt tttccaaact cctgcggttt ccaattgatt ttgttgtccg ggtcagtaaa
     1561 agtacccggc tcacccagaa gtttgcttaa aacgacatca ggagttccgt tgttttgtcc
     1621 tttagagaaa gaaagacaag tgtttgcaca tctttgcaca ggactgctga aataattcca
     1681 gttgccggtt ccgtggaaca atcttcctaa tttttcagac atttcgtgtc ccttttcgtt
     1741 taggtcaaca tccattcccc atgttccttc aggaatctta tctctggcag catgtcttaa
     1801 aagcatgact actttttcac cgccgtttat ccgttttctt atctcttcaa tatcaataca
     1861 tggatcaatt acagggaggt gttcaaaatg tccgttttca aacaatctga ctattcgacc
     1921 tttctttgtt ttgcaaagtg cagcaccatt gtaaaaaggt tctgctgaca ggaaacggtg
     1981 atcatataaa ggattcccgg ctttatctat gtgatagaca ccgctgtcat ctgctacaac
     2041 agcataccct ttatgaaaaa catcggctga aaggaattgt ttattgtgca attgagtccc
     2101 atcttcaaat atatgccatg cttttccatt tctgtgtaca actgcgattc cataacggaa
     2161 atccccggca tactcgtatc tttcccggta tttgtctttg ccgtcttttc caatgtgaaa
     2221 aaatcctttt acatctctaa cgacacagca gcccacctgg aaattaccgc tccagataaa
     2281 agatctgtcg tgaactggca caccggcggt gtcaatgtgg aagtttttgc cagattttct
     2341 aacactggca attccttcat aaaaaccgaa agtttcatca tatctttcat gataaagagg
     2401 gcttccgtca gctttaatat gataagcacc ggtttcatct gaaacagcgg ccaatccttc
     2461 gggccagtga aagctcatcg cttttttaaa agttttatta aaacatcttt ctccgttgca
     2521 aaggtagtcg ttttctgaat gatttaaaat gggtttgttt tcttcttttt tcatcttatc
     2581 ctctcaagtt ttttaaagac tggtgtgcat tttctcgcat ttaaaagaag aggctgtcaa
     2641 caactttaaa gatactccat agattgataa aacaggtttc atttatcgtt aaaattcaaa
     2701 tgcttaaatc tatattcatc tgtacagcaa tcacagattg atgaaattgg agtgttccca
     2761 agaaattcac gttttttgtt ttgaagttca acgaagctga tttctgtaac attaccaaaa
     2821 ctattattgc cgttaatatc gttacagcac agaatcaaca aaccatttgt gtctatgaag
     2881 ctgtcaatga aaaaagttcc gcaagtaata atttcatttg aatttctatc tttatataat
     2941 ttatttgccc tattgtggat ttttttgaaa aagatttcat aattattttc taacaaatct
     3001 gcgtaaatat tatcaggtaa catcggtgta gtttcataaa taatattaaa tcgtattttt
     3061 atattatcaa tgtttttggt catttcaata tactttgcaa tttcatttgt ttcgtccctt
     3121 tgtaatatgc ttatttgcaa tctttccaaa cccgcatcaa acaattctat tgttttttct
     3181 gtagtcagta gttttccatt agtcaagatt gaaacgccaa cgcttttttt tgacaattta
     3241 tttacaaaaa gcgcgcagtc tttgtgtagt aagggttccc caaaaccagc aaaaattaca
     3301 tcacagtttt gaggtaacca gtcgcatact ttatcaaata tactaattga catatcccta
     3361 ttctttcttt tgagattttg acgtggacaa caataacaat gcttgttaca tcgtgttgtt
     3421 agctcaatgt caaaaagttg tctgccgttt aatagtttaa tctgtgacct tctgatttgc
     3481 tgttcgttca tcgttcgtta tacttcccca aaaaaagtgt acacattttt ttatgcagcc
     3541 ccttttaatt tattcatttt ataaatcatt tcaaattctt tcggggaact taaaaaatat
     3601 caggatgaga aaaaatcacg gtgttctttt ccttgatgaa tttaaccatc tttctaaggc
     3661 gatacaaagc aaacttcttg tagctttaca aacagatgcg gaaggaaatt tctcgttttt
     3721 gtactttctt cctcaatctg ctaaaataat ttaattcttc ttttggatgg gctgtattaa
     3781 ctcccttcaa ccccatatcc cttccccacc ataaaccacg accttcgttt ctatctgtct
     3841 gggtcggaaa aatatgaaag aaaacggttt ctcactgcag gttatatgaa gtttgttatt
     3901 gtgttgttca actttcagta tttccaggtt gccgcactct gttaaggttg agatcctttc
     3961 cccgtcgtga tcccagagat tcggggg
//
LOCUS       DAOOYB010000266         3985 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_26833_length_3985_cov_11.010178, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000266 DAOOYB010000000
VERSION     DAOOYB010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3985
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26833_length_3985_cov_11.010178"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="PL056_11865"
     CDS             complement(<1..292)
                     /locus_tag="PL056_11865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47758.1"
                     /translation="MAKVSKAKQRLFTVLFMFTVSAFFIGILSFVYLFTKSTVVRNES
                     LFLKKSVLYTAQIKVSDSPVEIEKVYKEKVEEIKDADGNIQYYSVVADSGETV"
     gene            complement(292..1284)
                     /locus_tag="PL056_11870"
     CDS             complement(292..1284)
                     /locus_tag="PL056_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HPM47759.1"
                     /translation="MSENPAVTQGNKKPLISWQLPMKRVLWALAPTALAGVYFFGLRV
                     LLLYIVVHIAGFLSEYAFAKKYNQQVSSSVFVTNMLFAMIIPPALPFWMAAVGIIFGV
                     VFGKMVFGGFGKNIFNPALVGRAFIYVTFAVYMTGMWSEPYGGMMGGLLSYSPDAVTS
                     ATVLSSAKGTMNSIWLNAFLGNIAGSAGETSALLIILGGMYLVIKKVASREIIITTVI
                     GLVIPQTIFWYFGVKDAVDPLTAALTGGFMLGLFFMTTDPVSAPKTDTGKKLYGLFIG
                     LMTSLIRTFSVWPEGMMFAILLGNMLGPITDYAITERQKKKKAQLSSQTVGEVK"
     gene            complement(1309..3015)
                     /locus_tag="PL056_11875"
     CDS             complement(1309..3015)
                     /locus_tag="PL056_11875"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HPM47760.1"
                     /translation="MKIKLTTYIAICFFFIPSILFATLSGKKICVDPGHGGSDPGAIG
                     TNGSALPNEADLVLDVDLRLQSMLTDDAATVVMTRTDEVEVSLANRVAIANSNSVTIF
                     VSTHLNSSDLASANGTETYAYQSGGNSQTLATYIQNELLDHLGRTNRGVKYSGFYVIK
                     NTTMPAALSEGLFVSNSTEFNLISQAATRQEHALGVYHAICSYFGVTPKDDPGTGETP
                     GEVKGFAYNETKGGNVEGNRIAGADCYLKKSGEPDVHVVTSATGLFAFSSVDPGDYTL
                     LIEKTGFNSATKDVTVTSGNPTWASTGLTESTGGTETAWIKGFVFNDSNGLGNDPENR
                     IANATCVLKKTVGGAEVTVTTDDSGFFRFNDLEPADYTLTVSKTGFVTTSDKPLTLIA
                     GENWASTGIAEQGAVELGGLSGTVTSKTSYLAIAGAQCVITSKTSGDPYTVYSDETGK
                     YEFTDIIPDDYTLAVSADGYEKWTGDVTVMTDQNAVKDVELTPAETANDDDVSEIPDE
                     NETVNDTDVVETVDNETPDESVIDPDDDPGITDSDETDDSIWDDSGEIDEEVGCGCSF
                     ID"
     gene            complement(3025..3957)
                     /locus_tag="PL056_11880"
     CDS             complement(3025..3957)
                     /locus_tag="PL056_11880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47761.1"
                     /translation="MKRFLFAIIALSLISCVEQLSTTAQSSSDEVIVNEVKCTKGEFS
                     PDGKFIGFTGEKYKGLFVKDLSTNEIVKITDADGAGWRFSWSPDSRGITFRETEYLEK
                     SATYKIMKRFLNKESNELVGEFENTVWPPLWKDTIYSVDTFRSSNIALAVKPLSILKN
                     PAKPLKFNAFTSDNRVSLIDLKTGILKNLEEGTHSPSISDDGRFVLYIHLDTIKVLDT
                     VKNTTIEIGRGSSPSWVSGSKKVVYTSTLDDGRNITFSQIFLFDMISRVSETIAVPAE
                     RIPLAPAISSDGSKLLYTDYITGHLFIKKLGNEK"
BASE COUNT         1100 a          902 c          788 g         1195 t
ORIGIN      
        1 caactgtttc accgctgtct gcaacaactg agtaatattg gatattgccg tcagcatctt
       61 ttatctcttc aactttttct ttgtaaactt tctcgatctc aacaggcgaa tcactcactt
      121 taatttgtgc agtgtaaagt accgatttct ttaagaaaag tgattcattc ctgacaactg
      181 tgctttttgt gaaaagatag acaaaagaga gaattccaat gaaaaaagct gatactgtga
      241 acatgaaaag cactgtaaaa agacgctgtt ttgccttgct tacctttgcc atcacttcac
      301 ctcgccgaca gtttgagagg aaagttgcgc tttctttttc ttctgccgtt ctgtgatagc
      361 ataatcggtg atcggaccga gcatatttcc aagcagaatg gcaaacatca tcccttcagg
      421 ccagactgag aaagttctga tgagcgatgt catgagccct atgaaaagac cgtagagttt
      481 ctttccagtg tcagttttag gagcactcac aggatcggtc gtcataaaga aaagtccgag
      541 catgaatcca cctgtaagtg ctgctgtaag aggatcaact gcatctttta caccaaaata
      601 ccagaatatt gtctgcggga taactaaacc gatgactgtt gtgataatga tctctctgct
      661 tgccactttt tttatgacaa gatacattcc gccaagaatt atgagaagag ccgaagtttc
      721 acctgctgag ccggcgatat tgcctaaaaa tgcattcagc cagatactgt tcatcgtccc
      781 ttttgcactt gaaagcactg tcgcacttgt caccgcatcg ggtgaataac tgagaagtcc
      841 gcccatcatg ccgccatagg gttccgacca cattcctgtc atataaactg caaaagttac
      901 ataaatgaac gctctgccga caagtgccgg gttaaagatg ttctttccaa agccaccaaa
      961 aaccattttg ccgaaaacaa ctccgaaaat aataccgaca gccgccatcc agaaaggaag
     1021 cgcgggagga attatcatcg caaaaagcat atttgttaca aagacagatg atgaaacctg
     1081 ctggttgtac ttttttgcaa aagcatattc cgacaaaaat cctgcaatgt gaacgacaat
     1141 gtaaagaagc agaactctca acccgaaaaa atagaccccg gcaagagcag tcggggcaag
     1201 cgcccacagc acccttttca tcggcaactg ccaggatatc aacggttttt tatttccctg
     1261 tgttactgca ggattttcgc tcattagacc ctcgtatttt caatcgtttt aatctataaa
     1321 agaacagccg caaccgacct cttcatcaat ttcaccggaa tcgtcccaga ttgaatcatc
     1381 agtttcatca gaatcggtaa tgcccggatc gtcatcagga tcgatcacgc tttcatccgg
     1441 agtttcgttg tcaactgttt ctacaacatc tgtatcgttc accgtttcat tttcatcggg
     1501 aatttctgac acatcatcat catttgcagt ttcagccgga gtaagttcaa catctttaac
     1561 agcattctga tcggtcatta cggtcacatc gcctgtccat ttttcataac cgtctgcact
     1621 gactgccaga gtataatcat caggaataat gtctgtaaat tcatattttc ctgtttcatc
     1681 ggaataaact gtgtaaggat ctcctgaagt ttttgaagtg atgacacact gtgctcctgc
     1741 aattgcgaga taacttgttt ttgatgtgac tgtcccgctt aatccgccaa gttcaactgc
     1801 tccctgttca gcaattccgg ttgatgccca gttttctccg gcaataagtg tgagcggctt
     1861 atctgatgtt gttacaaagc cggttttgct tacagtcaga gtatagtcag ccggctcaag
     1921 atcgttaaat ctgaaaaatc ctgaatcatc agtagttaca gtaacttccg caccgccaac
     1981 tgtctttttc agaacacatg tcgcatttgc gattctgttt tccggatcat ttccaagacc
     2041 attcgaatca ttaaacacaa acccttttat ccatgctgtt tccgttccgc ctgtgctttc
     2101 ggtaagtccg gttgaagccc atgtcggatt tccgctcgtt actgttacat cctttgttgc
     2161 tgaattaaaa ccggttttct ctattaaaag tgtgtaatcg ccgggatcta ctgaagaaaa
     2221 agcaaaaaga cctgttgccg atgttacgac atgaacatcc ggctcacctg attttttaag
     2281 ataacagtcc gcccctgcga tcctgttccc ttcaacattc ccgccttttg tttcattgta
     2341 tgcaaaacct tttacttcgc ccggagtttc gccggttccg ggatcatctt tcggtgttac
     2401 gccgaaatag ctgcatatcg catgatagac accgagagca tgttcctgtc ttgttgcagc
     2461 ttgcgaaata agattgaact cagttgaatt tgacacaaaa agcccttcag aaagtgcggc
     2521 aggcattgtt gtgtttttaa tgacataaaa tccggaatat tttactccgc gattagttct
     2581 tccaagatga tcaagaagtt cattctgtat gtaagttgca agtgtctgac tgttgccgcc
     2641 tgactggtat gcatatgttt cggttccgtt tgctgaagca agatcacttg aattaagatg
     2701 ggttgaaaca aatattgtga cactgttgct gtttgcaatc gctactctgt ttgcaagtga
     2761 cacctcaact tcatcagttc ttgtcattac cacagttgcg gcatcatcag tcagcattga
     2821 ctgaagccgg aggtcgacat caaggacaag gtctgcttcg ttgggaagag ctgaaccgtt
     2881 tgtccctatt gctccagggt cactgccgcc gtgaccgggg tcgacacata ttttctttcc
     2941 tgaaagtgtt gcaaaaagta ttgaagggat gaaaaagaag caaatcgcaa tgtatgttgt
     3001 taattttatt ttcattttgc cctcctactt ctcatttccg agttttttaa tgaagagatg
     3061 cccggtgatg tagtctgtgt agagaagttt cgatccatcg gaagagatcg ccggcgcaag
     3121 aggtattctt tctgcaggaa cagcaatggt ttctgacaca cgggaaatca tatcaaaaag
     3181 gaatatttga gaaaaagtta tgtttcttcc gtcatcaaga gttgaagtat aaacaacttt
     3241 ttttgaaccg gaaacccagc ttggtgaaga gccgcggcct atttcaattg ttgtattttt
     3301 aactgtgtca agaaccttaa ttgtgtcaag gtgaatataa agaacaaatc tgccgtcatc
     3361 cgatattgac ggagagtggg ttccctcttc cagatttttc aaaatacctg ttttcaaatc
     3421 aatgagcgat acccggttgt cagaagtgaa cgcattgaat ttcaggggtt ttgcggggtt
     3481 tttaagaatt gaaagcggtt ttactgcaag agcgatattt gatgatctga acgtatcaac
     3541 tgaatatatc gtgtctttcc agagcggcgg ccacacagtg ttttcaaatt cacctacaag
     3601 ctcattgctc tctttattaa ggaacctttt cattatttta taagttgctg atttttctaa
     3661 atattccgtt tccctgaaag taatcccgcg actgtcaggc gaccatgaaa atctccatcc
     3721 cgcaccatct gcatcggtaa ttttaacgat ctcatttgtt gagagatctt taacaaaaag
     3781 ccctttgtat ttttcaccgg taaagccgat gaattttcca tcaggcgaaa actctccttt
     3841 tgtacacttc acttcgttca cgatcacttc atctgagctg ctctgagccg tggtcgataa
     3901 ctgctcgaca cacgaaatca atgaaagtgc aataattgca aataaaaacc ttttcatcat
     3961 ttaccccaca aaaaaataac agaca
//
LOCUS       DAOOYB010000267         3979 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_26902_length_3979_cov_12.352701, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000267 DAOOYB010000000
VERSION     DAOOYB010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3979
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26902_length_3979_cov_12.352701"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="PL056_11885"
     CDS             <1..580
                     /locus_tag="PL056_11885"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HPM47762.1"
                     /translation="KLLKAIPEDVILAVDEAYVDFVDADDYPNSLNYRNIHKNTIVTR
                     TFSKAFGISGIRLGYGIATAELVDYMNRVREPFNTSSLAQAAGIAALNDSEYLKRSVD
                     VNKSGKEFYYKEFQRLGLKYLPTQGNFILVKVGNEPTVGMECFNYLMKQGVITRPMGG
                     YGFPNWIRISIGLEDHNRICIEKLENFLSKKA"
     gene            577..1284
                     /locus_tag="PL056_11890"
     CDS             577..1284
                     /locus_tag="PL056_11890"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HPM47763.1"
                     /translation="MKHLTLILFFVVFTGCRGTYDTALKKQGYEPDGLGFIQAVQDNN
                     AKAVGWFLKMPEVLNFSDKTGATAIFYAAGLDTPAMLNTLITAGLPFELRDNSKKNPL
                     HYAVESGCVENVKSLIDKGVDPSWQDNFERDILNTLISFRKNENLEIFELLIKAGSNI
                     NLKDIDKKTPLIIAAEKGHSGYMKRLIELKASPLHKDYKGKTALDYATENYDKKVIGH
                     EIFNLLSDYTNGIKGNL"
     gene            complement(1293..2609)
                     /locus_tag="PL056_11895"
     CDS             complement(1293..2609)
                     /locus_tag="PL056_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HPM47764.1"
                     /translation="MVKTRKIRDLNVWMNGELVGRWSNFSKGIHEFRYYDSWLESVNA
                     RPLSLSLPLVSENILYKGAVVENYFDNLLPDSIEIRRRIQSRFSADSMEPFDLLAQIG
                     RDCVGAVQLLPVGTEPEEIMKIVSEPMDEEQVAFILKSVTSAPFPGMRESKNFRISVA
                     GAQEKTAFLSNNGKWSIPLGSTPTTHIFKLPIGKIMGADLSTSIENEWLCSKITKAFG
                     IESAESEIGIFKDQKVLIVKRFDRAFSSDSKWIIRLPVEDMCQVTATSPFEKYETDGG
                     PGIEEIMKILLGSKNSLKDRHAFFKTQILFWMLAAPDGHAKNFSIFLEKQGGYRLAPI
                     YDVISVYPVMGKKSFEIAPEKLRMAMAVTGKNRHYDWMNIKKRHWDETALRCGVHNFV
                     EEVITELVEKTPGVINEVSKILPPDFPETVSEKIFEGLEKAAKKLK"
     gene            complement(2611..2850)
                     /locus_tag="PL056_11900"
     CDS             complement(2611..2850)
                     /locus_tag="PL056_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPM47765.1"
                     /translation="MEYLIFTDFQLAKSLKNRRIELKLTQKAVAEKVGLLPKTVSALE
                     NNPEKCSVESLMKYLSALDLHIVLKAKNSNVTEGW"
     gene            complement(2957..3139)
                     /locus_tag="PL056_11905"
     CDS             complement(2957..3139)
                     /locus_tag="PL056_11905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47766.1"
                     /translation="MEKSALIIFLIFVFAQIQGDEKSYNFDLYTLDDVLYSTNQAEYC
                     FKAKNTLNSLRKQKKP"
     gene            complement(3153..>3979)
                     /locus_tag="PL056_11910"
     CDS             complement(3153..>3979)
                     /locus_tag="PL056_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47767.1"
                     /translation="ENIDEFDGEEQDEFEHDEEVDIDEQSDDDSGYDHVEIINNTDGS
                     VTVNVTLNNEKDIVSSYLMDAGTSSWNYFYGDFVNIGFRVYDLLEDQYVAYHTAFRFT
                     DVEVPAGATIEKAELSLHPTNEVDSSKRMYLRLAMEKTNNSSAFDTSNYSSGRPDQRT
                     KTDAVVAEWLLRCQDIDDCYDPESSYCKQRALDCWDREVRYTVPKSLKEMAQEVIDIA
                     GWTKGNAMTVFITGTYPSEMPNEEKPNYSNSRSVTGFDTEKTQSYYPVLSVTFTVK"
BASE COUNT         1165 a          837 c          739 g         1238 t
ORIGIN      
        1 aaaacttctc aaagcgatcc ccgaagatgt aatacttgca gttgatgaag catatgtcga
       61 ttttgttgat gctgatgatt atcctaattc gctgaactat agaaatattc ataaaaatac
      121 gatagttaca agaactttct caaaagcttt cggcataagc ggcattcgtc tcggatatgg
      181 aattgcaacc gctgaacttg ttgattacat gaaccgcgtc agagaacctt tcaacacgag
      241 ttcgcttgct caggcggctg gaattgcggc gttaaatgat agtgaatatc tgaaaagatc
      301 ggttgatgtc aataaatctg gaaaagagtt ttattataaa gaatttcagc ggcttgggct
      361 taaatatctt ccgacacagg gcaactttat tctcgtaaaa gttggaaacg aaccaactgt
      421 cggaatggaa tgtttcaact atctgatgaa gcagggggtt atcaccagac cgatgggcgg
      481 ttacgggttt ccgaactgga tcagaatttc aataggtctt gaagatcaca acagaatatg
      541 tattgaaaaa ttagagaact ttcttagtaa aaaagcatga agcatttaac tttaatactg
      601 ttttttgttg tttttacggg atgcagggga acttatgata ctgctttgaa aaaacagggt
      661 tatgaaccgg acggactggg ttttattcaa gccgttcagg ataacaacgc aaaagctgtg
      721 ggctggtttt tgaaaatgcc ggaagtgctt aatttttctg ataaaactgg cgcaacggcc
      781 attttttatg ccgcagggct cgatactccg gcaatgctta atacacttat taccgcaggc
      841 cttccttttg aactcaggga caactcaaaa aagaacccgc ttcactatgc agttgaaagc
      901 ggatgtgttg aaaatgtcaa atctctcatt gataaaggag ttgatccgtc atggcaggac
      961 aactttgagc gagacattct caacactttg atatcattta gaaaaaatga aaatttagag
     1021 attttcgaac tcctgataaa agccggaagc aacatcaatc ttaaagacat tgacaaaaaa
     1081 actccgctta tcattgccgc cgaaaaagga cattccggct atatgaaaag actcatcgaa
     1141 ctcaaagcat cccctcttca caaggattat aaaggaaaaa ccgcacttga ctatgcgacc
     1201 gaaaactatg acaaaaaagt tatcggacac gaaatcttca atcttctaag tgattacacc
     1261 aacgggatca aagggaatct gtagaaatct tattatttca gcttcttagc cgctttttca
     1321 agtccttcaa aaatcttttc tgaaactgtt tccggaaaat cgggtggcag gatttttgaa
     1381 acttcattaa ttacaccggg agttttttca acaagttcgg tgattacttc ttcaacaaaa
     1441 ttatgtactc cgcatcgtaa agctgtttca tcccagtgtc tttttttaat gttcatccag
     1501 tcatagtgtc tgtttttgcc tgttacggcc attgccattc tcagtttttc gggagctatt
     1561 tcaaaagatt tttttcccat tacgggataa actgaaatga catcatatat aggggcaagc
     1621 cggtaaccac cctgtttttc taagaaaatg ctgaaatttt ttgcatgacc gtcaggcgcc
     1681 gcaagcatcc agaaaagtat ttgggtttta aaaaaagcgt gtctgtcttt taaggaattt
     1741 ttagatccca gaagtatttt cattatctct tcaattccgg gacctccgtc agtttcatat
     1801 ttttcaaaag gagaagtagc tgttacctga cacatatctt caaccggaag ccggattatc
     1861 cacttgctgt ccgacgaaaa ggccctgtca aatcttttga cgatcagaac tttctgatcc
     1921 ttgaaaattc cgatttcgct ttctgccgac tctattccga aagcttttgt aatctttgag
     1981 cacagccatt cgttttcaat cgaagtgctc agatcggcac ccattatttt gccgataggg
     2041 agcttgaata tgtgagttgt gggagtagaa cccaatggaa ttgaccactt accgttattt
     2101 gaaagaaatg ctgttttttc ctgtgctcct gcaactgaaa tcctgaaatt tttgctctcc
     2161 ctcataccgg gaaatggagc agaagtcaca gattttaaaa tgaaagctac ctgctcttca
     2221 tccatcggct ctgaaacaat tttcattatc tcttccggct ctgttccgac aggcagaagc
     2281 tgaacagcgc cgacacaatc acgaccaatt tgtgcaagaa gatcaaatgg ctccatcgaa
     2341 tctgctgaaa acctgctttg aattcttctt ctgatctcaa tactatcagg gagaaggttg
     2401 tcaaaatagt tttcaacaac cgcacctttg tataaaatat tttcagacac aagcggcaga
     2461 gaaagagaaa gaggtcttgc atttactgat tcaagccagg aatcataata tctgaactca
     2521 tgaatccctt ttgaaaaatt agaccaccga ccgacaagct ctccgttcat ccagacattc
     2581 aagtccctga tctttctcgt ttttaccatt ttaccaaccc tctgtcacat tggagttttt
     2641 tgctttcaat acaatgtgca aatccagtgc ggacaaatac ttcatcaagc tttcaacaga
     2701 acacttttca ggattgtttt caagcgccga cactgtcttt ggaagaagac cgactttttc
     2761 tgccacggct ttctgagtca atttcagttc aatccgcctg ttttttaatg atttagcaag
     2821 ctgaaaatcg gtaaagatca aatattccat attttccccc acaaggtata acacagcaat
     2881 ataccccaaa aggtataatt tgtcaatatc ccccgaaggg tattttttaa gaattattta
     2941 accggttttt ctgcattcag ggtttcttct gttttcttaa tgaattcagg gtgtttttcg
     3001 ctttgaaaca atattctgcc tgatttgtcg agtagagaac atcatcgagg gtataaagat
     3061 caaaattata cgatttttca tctccctgta tctgcgcaaa cacaaaaatt aaaaaaatga
     3121 ttaaagctga cttttccatt ctttttcccc aattatttca ccgtgaatgt tactgaaaga
     3181 acgggataat atgactgtgt tttttctgtg tcaaaaccag tgactgagcg gctgtttgaa
     3241 tagttcggtt tttcttcgtt cggcatttct gaaggatatg tgccggtgat gaaaactgtc
     3301 attgcgttcc cttttgtcca gccagcaata tcaattactt cctgagccat ttcctttaat
     3361 gatttcggga ctgtatatct tacttccctg tcccagcagt caagtgctct ctgtttgcag
     3421 taacttgatt caggatcata acaatcgtct atatcctgac acctcagaag ccattcagca
     3481 acaactgcat cggtctttgt tctctggtca ggtcttcctg aactataatt tgatgtatca
     3541 aaagcacttg aattgttggt cttttccatc gcaagacgaa gatacatcct ttttgatgaa
     3601 tcaacttcat ttgtcggatg taatgacaat tccgcttttt caattgtcgc ccctgccgga
     3661 acttcaacat cggtgaatct gaaagcggtg tgatatgcaa catactgatc ttcaaggaga
     3721 tcataaactc tgaaaccgat gtttacaaaa tctccgtaaa aatagttcca ggaagatgtt
     3781 cccgcatcca tgagatagct tgaaacgata tctttttcgt tgttcaaagt tacattgacc
     3841 gtcaccgaac cgtcagtgtt gtttatgatc tcaacatggt cataaccgct gtcatcatcg
     3901 gattgttcat caatatccac ctcttcatca tgctcaaatt catcctgctc ttcaccatca
     3961 aattcatcaa tgttttcta
//
LOCUS       DAOOYB010000268         3959 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_27095_length_3959_cov_7.029713, whole genome shotgun sequence.
ACCESSION   DAOOYB010000268 DAOOYB010000000
VERSION     DAOOYB010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3959
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27095_length_3959_cov_7.029713"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="PL056_11915"
     CDS             <1..320
                     /locus_tag="PL056_11915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47768.1"
                     /translation="IYYYKTGSGKEVDFCWIDRTGKKNLVQVSFDMNDEKTKKREVSA
                     LFEAMGEINEKNATLVTMNESGVLELEDRTIRIVPAWEYLIKFEYSEKSVFQINDFML
                     GGD"
     gene            324..1205
                     /locus_tag="PL056_11920"
     CDS             324..1205
                     /locus_tag="PL056_11920"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HPM47769.1"
                     /translation="MENIKLFRDFLLHEKRYSKNTADGYCKDIELMQRYFKETGLEIE
                     ELDKDDLKDYFGAIYSEVKPSSLKRKIAAIRQFYILLKKREIIEENPALTLSGPKNSG
                     ILPGALSRDEMLKLIDFPYKNDLMGLRNRAIIELLYSSGVRVGELVSLKMKNLNLRNR
                     TINVLGKGKKERLIPVTHQAVESIENYIMKKEGGKEPESIIFCNLKGLGLTERGVQYI
                     IDRISREAGIFRTITPHMLRHSFATHFLENGMNLRYLQHLLGHSNLSTTEIYTHLSID
                     QLKKVYNKAHPGSRKDK"
     gene            1281..3176
                     /locus_tag="PL056_11925"
     CDS             1281..3176
                     /locus_tag="PL056_11925"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HPM47770.1"
                     /translation="MTVIGIDLGTTNSCVAVIRNNQSEVIKDAGGRNTIPSIVSFIKN
                     ESTGDVEKSCGHAAYQQMLINTENTVYCIKRLMGHSFKSPVVNQLIPTLSYKIIPSEK
                     GDGLAINIPVCNRSFSAEEISSFILTELRSTAEKYLGETVSKAVITVPAYFNNKQRQA
                     TKDAALIAGIEVLRIINEPTSAALAYGFSQGTEEEKLLAVFDFGGGTFDITIMEVSKG
                     TFNVVATAGNTFLGGEDITNTLFEYFLEDLASNYGVTDLDDPLVLQRLRDYAETTKKN
                     LTIQTRATVEIPYLKEVEGTFIHYKVEVTREKIEELAMPFVRKVIKTFEKTLKEVRLE
                     AKALAGIILIGGQTRMPLIQNALLEFTPDVPILKNINPDETVAIGAAIQGKMLDDESK
                     NEDVDMLLLDVTPHNLGIAVGKDMFYTLIQKNSTVPTSVDDIFVTSRDNQDKARILLL
                     QGNSLVASENEVLGEFELDNLRPAKRGEVKIKITYEIDMNGIVTVQATDMDTGTSQKI
                     TVASTGNLTKEELSKKIEENSDYYLELAEKTMIEEIVQSIENYLYDLEKMKPKLYQIF
                     SSSKVGVEKMDKMEILISGTKAYLKNDSLKLEKLQDIETKLDTLKNTYKSLIEQSDES
                     MGPGGLF"
     gene            3274..>3959
                     /locus_tag="PL056_11930"
     CDS             3274..>3959
                     /locus_tag="PL056_11930"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPM47771.1"
                     /translation="MIKLIKSKIGSDIDTLNGVRTGSFNNLDKFAVISIVVLSALLYI
                     PFLGNFFMIDPWENHYSHVAWETLERGSFAKLWYQNSNRFWSKPPLLFWMLMPFYKLH
                     ISEFMGRLPIALFSIGTLAGFYILLTRLVSRRASMLATLILMLSPHYFMLSRQIMVDL
                     PFIGLNTIALLCMAIYFVGDIPEEEKIWKIPRKDMYLYLFYFFEGWGFLAKGLLSVVI
                     PGTALFVFML"
BASE COUNT         1280 a          716 c          848 g         1115 t
ORIGIN      
        1 caatttatta ttacaaaacc ggatccggaa aggaagttga tttctgctgg atagatagaa
       61 ccggaaaaaa gaaccttgtt caggtttcat ttgacatgaa cgatgaaaag acaaaaaaaa
      121 gagaagtttc agcacttttt gaagcaatgg gtgagatcaa tgaaaaaaat gcgacacttg
      181 taacaatgaa tgaatcaggt gtgttagaac ttgaagacag aacaattcga atagttccgg
      241 catgggaata tctgataaaa tttgaatata gtgaaaaaag tgtgtttcag ataaacgatt
      301 ttatgctcgg tggagattaa catatggaaa acataaaact tttcagagat tttctgctcc
      361 acgaaaagag atattcaaaa aatacggctg atggatactg taaagatatt gaactgatgc
      421 agagatattt taaagaaacg ggtctcgaga tagaagaact cgataaagat gacctgaaag
      481 attatttcgg tgcgatatat tccgaagtga agccatcttc gcttaagcgg aagatcgctg
      541 cgatcagaca gttttacatt cttttgaaaa aacgggaaat aatcgaagag aacccggcac
      601 ttacattgtc cggtccgaag aattcaggca ttctgcccgg agcgctttca agagatgaaa
      661 tgctgaaact cattgatttt ccttataaaa acgacctcat gggattgcgt aacagagcaa
      721 taatagagct tctttacagc agcggagtcc gtgtgggcga acttgtgtcg ctcaaaatga
      781 agaacctgaa tttgaggaac agaacaataa atgtcctcgg aaaagggaaa aaagagcgac
      841 ttattcccgt aactcatcag gcagttgaat caattgaaaa ttatattatg aaaaaagagg
      901 gcggtaaaga gcccgaatca ataatattct gtaatcttaa agggttgggt cttacagaaa
      961 ggggagttca atatattatc gaccgcattt caagagaggc gggaatattc agaaccataa
     1021 ctccgcacat gctcagacac agctttgcaa cgcacttcct tgaaaacggg atgaacctta
     1081 gatatcttca gcatcttctc gggcacagca acctttccac gacggagatt tacacccacc
     1141 tttcgataga tcaacttaaa aaagtttata acaaagctca ccctggttca agaaaagata
     1201 aatgaaatta caaaaaactt gagattacaa aagaaatact ttaaaatgcc ctctgtaaat
     1261 gttccataaa cggagttgat atgaccgtaa tcggtattga tctgggaacc acaaattcat
     1321 gtgttgctgt aatcagaaac aaccagtctg aagttattaa agatgccggt ggaaggaaca
     1381 ccattccgtc catagtcagc ttcattaaga acgaatcgac gggagatgtc gaaaagagct
     1441 gcggtcatgc cgcatatcag cagatgctca taaacaccga gaacactgtg tactgcataa
     1501 aacggctcat ggggcacagt tttaaaagcc ctgttgtaaa ccagcttatc ccgacacttt
     1561 catataagat aatcccgagt gaaaaaggtg acggtcttgc gatcaatatt cctgtctgta
     1621 atagaagttt ctcggctgag gagatatcat cgttcatcct gactgagctt agaagtacag
     1681 ctgaaaaata tcttggcgaa acagtctcca aggcagttat taccgttccg gcatatttca
     1741 acaacaagca gagacaggcg acaaaagatg ccgcactgat tgcgggaatt gaagttttga
     1801 gaatcattaa cgagccgaca tctgcagctc tggcatacgg tttttctcaa gggacagaag
     1861 aggaaaagct tcttgctgtc tttgatttcg gcggaggaac attcgatatt acgataatgg
     1921 aagtttctaa agggacattt aatgttgttg caactgccgg aaatacattt ctcggtggag
     1981 aggatataac aaacaccctt tttgaatatt ttcttgagga ccttgcatca aattatggtg
     2041 tcacagacct tgatgatcca cttgttcttc agcgtttaag ggattatgcc gagacaacaa
     2101 agaaaaatct gaccattcag acaagggcga cagtcgagat accttatttg aaagaagttg
     2161 agggtacatt tattcactat aaagttgaag tgaccagaga aaagatagaa gaacttgcca
     2221 tgccctttgt aagaaaggtg attaagacat ttgaaaaaac actcaaggaa gtgcgtcttg
     2281 aagcaaaagc tctcgcaggg atcatactta taggtggaca gaccaggatg ccccttattc
     2341 agaatgcact tcttgaattc actcctgatg ttcctatttt aaagaacatc aaccctgatg
     2401 aaactgtcgc tatcggtgca gccattcagg gaaagatgct tgatgatgaa tcaaagaacg
     2461 aagatgttga tatgctcctt ctggatgtca ctccgcacaa tctgggaatt gctgtcggaa
     2521 aggacatgtt ctatacgctg attcagaaaa attcaactgt tcctacttcg gttgacgata
     2581 tatttgtaac atcaagggat aatcaggata aagcgagaat tcttcttctt cagggaaatt
     2641 cacttgtcgc ttctgaaaat gaagttcttg gcgagtttga acttgacaat ttgagacccg
     2701 caaaaagagg cgaggtcaag ataaagataa cttacgaaat tgatatgaac ggaatcgtta
     2761 cggttcaggc cacagatatg gataccggta catcccagaa gatcacagtc gcatcgactg
     2821 gaaatctgac caaagaagag ctttctaaaa agatcgaaga aaactccgat tattaccttg
     2881 agcttgctga aaagacaatg atagaagaaa tagtacagag cattgaaaac tatctttatg
     2941 atcttgaaaa gatgaaaccc aagctttacc agatattttc ttcatcgaag gtcggtgtag
     3001 agaaaatgga caaaatggaa atacttattt cgggaaccaa ggcatattta aagaatgatt
     3061 ctcttaaact tgaaaaattg caggatatag aaacaaagct tgatacgctc aagaacacat
     3121 ataaaagtct tatcgaacaa tctgatgaat caatggggcc gggagggctt ttctaaaccg
     3181 cttaatatat aacggttttc ttgacttttt tcaatgtgat attaatttct catagattgg
     3241 gtcttattga gtttatgagc tgggggaact atcttgataa agttgatcaa aagtaagatt
     3301 ggaagcgaca ttgatacatt gaacggtgtg agaaccggaa gctttaacaa tcttgataag
     3361 tttgctgtaa tttccattgt tgtcctttct gcacttctct atattccgtt ccttggcaat
     3421 tttttcatga tagatccatg ggaaaaccat tattcacatg ttgcatggga gacacttgag
     3481 aggggatctt ttgcgaaatt gtggtatcag aactcaaatc gtttctggtc caagccgcct
     3541 cttcttttct ggatgctgat gcctttttat aaattgcata tttctgagtt catgggtcgt
     3601 ctgcctatcg ctcttttctc gatcggcact cttgcaggat tttatattct tcttacgcgg
     3661 cttgtttcaa gaagggcttc aatgctcgcg acacttatcc ttatgctgag tcctcattat
     3721 tttatgcttt caagacagat aatggtggat cttcctttta tcggacttaa tacgatcgct
     3781 cttctgtgca tggcgatata ttttgtcggt gatattcctg aagaagaaaa gatatggaag
     3841 ataccaagaa aagatatgta tttgtatttg ttctatttct ttgagggctg gggtttcctt
     3901 gcgaaagggc ttctttctgt ggttataccg ggaacagctc tttttgtttt catgctttt
//
LOCUS       DAOOYB010000269         3935 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_27325_length_3935_cov_14.298196, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000269 DAOOYB010000000
VERSION     DAOOYB010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3935
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27325_length_3935_cov_14.298196"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..946
                     /locus_tag="PL056_11935"
     CDS             74..946
                     /locus_tag="PL056_11935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47772.1"
                     /translation="MKVAILIFILFLLSCNSSEKTNTDNLSEEDNESSDELFICFDAT
                     DFYINGAGNESCPCQNDVDRIKRSINDVTGSEKGNTMKIEEILYLEDRNEVVPKGDCG
                     QENKCALTFKNYDRSLFKDSLIGKEWVLYLIEGFYGSVYGEYVSRDVQVIRHKDGTLI
                     AASGTGIVNEDGEHEWDDKVWPSKLVPEIKAEQKVLSTCESFCVKFQSGEDGKPLGDW
                     QYDHIIAPPVEITVEGKTPVIVKNGEVIRSEGYEYFVRDSVRVTEEDSDGYILSYGKK
                     YKFDFFIVNTEALK"
     gene            1089..1382
                     /locus_tag="PL056_11940"
     CDS             1089..1382
                     /locus_tag="PL056_11940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47773.1"
                     /translation="MKTIFFLMVLFFSVFSTACKIDQKLPYNAVVLDQIIFVDEVKEG
                     VPQTLLMPNGTTVTYKMPPKLHDGQLIKVRDVEGEDPYYIRISLRYRDEQSKK"
     gene            1363..1974
                     /gene="coaE"
                     /locus_tag="PL056_11945"
     CDS             1363..1974
                     /gene="coaE"
                     /locus_tag="PL056_11945"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HPM47774.1"
                     /translation="MNKAKSNVAALTGAIATGKSVVSNYLLKNFGFVVIDADKIGHEV
                     LEDTDIIRIIREEFGETVIREGAVNREKLGRIVFSDPSKLMLLNSIVHPRLFSKAQQM
                     IEAIPSSRPVIFEAAVLIEAGWHKYFDKIIITTCSPDIQIKRIVARDGISEADALKII
                     ASQISYDERYAFADYIVDTSEGLENITVSLDEIANDLISQKEV"
     gene            1975..3297
                     /locus_tag="PL056_11950"
     CDS             1975..3297
                     /locus_tag="PL056_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020081515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HPM47775.1"
                     /translation="MESKKFMGHPVGLFVLFFTELWERFSFYGMKAILVLYMVASIEK
                     GGLGWSNAKGLEIYGWYNMLVYVMAIPGGIIADRYLGQRKSVYLGGLLLCAGHLLLAY
                     PPEAAFFSALGLIVLGVGLLKPNISTMVGELYPDGDAAKDAGFTIFYMGINIGAFFSG
                     IAVGWLAYKYGWHYGFGLAGIGMIIGQIVYAYGQKYLGTAGLKKKVCIDAATSRPVKC
                     EFTAVEKRKIGLVIFSFLIVIVFWAAFEQAGGLMSLYTEQFTSRDLFGFTIPTAWFQS
                     LNPFYIMIFAPVVASFWIMLSRIGKEPSALTKMGLGTMILGIGFLFMVGASVERGGDE
                     LAKSSMLWLCLAYFFHTIGELALSPVSLSFITNMAPKRMTSQVMGWYFAVTGIAGYFA
                     SKIGIFAETAGELKVFGLIAGFTMFFGILLMIIAPKINKIADIHRTDG"
     gene            3312..>3935
                     /locus_tag="PL056_11955"
     CDS             3312..>3935
                     /locus_tag="PL056_11955"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPM47776.1"
                     /translation="MTDIKAVIFDWNGTLVDDVWLSVNAMNAMLEKRGKKPITTEYYK
                     SIFSFPVINYYKELGLDVENEWEIISREFMDIYLGASEKLKLFRDVEPVVKYFRELSF
                     TTGILSAMEHSILNKHLEHFRISQLFDFVQGIENHYAGGKLHLGRKILEKTGLKSEEI
                     LFVGDTYHDYEVAKEMGCKVVLINRGHNPYEMLKSTNAPVLNSLEELK"
BASE COUNT         1189 a          708 c          920 g         1118 t
ORIGIN      
        1 ttatatcgtc acttgattaa ttttggaatt tgatataatc taacccgtta attatttgaa
       61 tttttggggt tttatgaagg ttgcgattct gattttcata ctttttcttc tttcctgtaa
      121 ttcatcagaa aaaacaaaca cggacaatct ttctgaagag gataatgaaa gcagtgatga
      181 attatttatc tgttttgatg caactgactt ttacataaat ggcgcaggga atgaaagctg
      241 tccatgccag aatgatgttg atagaataaa aaggtctatt aatgatgtga caggatcaga
      301 aaaaggcaac acgatgaaaa ttgaagaaat cctttatctt gaagacagga atgaagttgt
      361 tcccaagggt gattgcggac aggaaaataa gtgtgcactg acattcaaga attatgacag
      421 atctcttttc aaagattcat tgatcggcaa ggagtgggtt ttatacttaa tagaaggttt
      481 ttatggaagc gtttatgggg aatatgtctc cagagatgtt caggttatta ggcacaaaga
      541 cggcactttg attgcagcaa gtggaacagg aatagtaaat gaagacggtg aacacgaatg
      601 ggacgataag gtatggccgt caaaacttgt tccggaaatc aaagctgaac agaaagtatt
      661 atcaacatgc gaatcattct gtgtaaaatt tcagagcgga gaagacggga aacctcttgg
      721 cgactggcag tatgaccata taattgctcc tccggttgaa atcacagttg aagggaaaac
      781 accggttatt gtcaaaaacg gtgaagtcat caggtctgaa ggatatgaat attttgtaag
      841 agacagtgtc agagtaaccg aagaagattc agacgggtat attttaagtt acggcaaaaa
      901 atacaagttc gatttcttca tagtgaacac cgaggctctg aaataaagat tttgaataca
      961 tccatttgga tgaacccaat agatgtttgt gttataatgt ggagataaga aacccgacat
     1021 ctcaaattta attgaactga aatcgtttta aaatataatt gaacggattg aacttccgga
     1081 ggtgtccttt gaaaacgata ttttttttaa tggttttgtt tttttcagtc ttttcaacgg
     1141 cttgcaagat cgaccagaaa cttccttaca atgcggtggt tcttgatcag ataatatttg
     1201 tcgatgaggt gaaggaaggg gttccgcaga cccttctcat gcccaacgga acgactgtta
     1261 catataaaat gcctccgaaa ctgcacgacg gacagcttat aaaggtccgc gatgtggaag
     1321 gtgaggatcc gtattatata agaatttcac tgaggtatcg tgatgaacaa agcaaaaagt
     1381 aatgtggcgg ccctcacagg ggcgatcgcg acggggaaaa gtgttgtatc aaactacctg
     1441 ttaaagaatt tcggatttgt tgtgatagat gccgacaaga tcggtcatga agttctggaa
     1501 gacactgata ttattaggat tatccgcgaa gaattcggcg aaaccgtgat aagggaaggg
     1561 gctgtgaacc gtgaaaagct cggcagaata gttttttcag atccttcaaa gctcatgctt
     1621 ttaaattcca ttgtccaccc cagacttttt tcaaaggcac agcagatgat agaggcgata
     1681 ccgtcatccc gtcccgtaat ttttgaggcg gcggtcctta tcgaagcggg atggcacaag
     1741 tattttgaca aaataataat caccacatgt tcgccagata ttcagataaa aagaattgtc
     1801 gcccgtgacg gtattagcga agcggatgca cttaaaatca tcgcaagtca gatatcctat
     1861 gacgaaagat atgcttttgc cgactatatt gttgacacaa gtgaagggct tgaaaacatt
     1921 acagtttcac ttgatgaaat tgcaaatgac cttatttctc aaaaagaggt ttaaatggaa
     1981 tcaaaaaaat ttatggggca tccggtcgga ttgttcgttc tcttttttac tgagctgtgg
     2041 gaaagattct ctttttacgg aatgaaagcg atacttgtcc tttacatggt ggcatctatt
     2101 gaaaaagggg gactcggatg gagcaatgcg aaaggtcttg agatctacgg ctggtacaac
     2161 atgcttgtct atgtcatggc gatccccggc ggaataattg cagacaggta tctcggtcag
     2221 agaaaaagtg tctatcttgg cggacttctg ctttgtgcag gacaccttct tctggcatat
     2281 cctcccgaag cggcgttctt ttcagctctg gggttgatcg ttctcggagt aggtctcctc
     2341 aagcccaata tttcaacaat ggtcggagag ctttatcctg acggtgatgc ggcaaaagat
     2401 gccggtttta caatatttta catgggaata aacatcggtg cgttcttttc agggatcgct
     2461 gttggatggc ttgcatacaa gtacggctgg cattacggtt tcggacttgc cggcatcgga
     2521 atgataatcg gtcagatagt ctatgcatac ggtcagaaat atctcggaac agcaggtctg
     2581 aaaaagaaag tgtgtatcga tgccgcaaca agcaggcctg taaaatgtga gttcacagca
     2641 gttgaaaaac ggaagatcgg gcttgtcatc ttttcatttc tgatcgttat tgttttctgg
     2701 gccgctttcg agcaggcagg cggattgatg agtctttaca ctgagcagtt tactagccgg
     2761 gacctgtttg gatttacaat tccgactgca tggttccagt cgcttaatcc tttttatatc
     2821 atgatatttg cacctgttgt cgcatctttc tggatcatgc tttcaagaat aggtaaggag
     2881 ccgtcggcac ttacaaaaat gggacttgga acgatgatac tcggtatcgg attccttttc
     2941 atggttggag cttcagttga aagaggtgga gatgaacttg caaaaagcag catgttgtgg
     3001 ctctgtctcg catatttttt ccacacgata ggcgaacttg ctctttctcc agtgtcactt
     3061 tcattcataa caaatatggc gccgaagaga atgacttctc aggtaatggg atggtatttt
     3121 gccgtgactg gaattgccgg ttattttgct tccaagatcg ggattttcgc agaaaccgcg
     3181 ggtgagctca aagtgttcgg actgatcgcc ggattcacga tgttcttcgg gattcttctg
     3241 atgataatag caccgaagat caataaaata gcagacatac acagaacaga cggttgacgg
     3301 agaaattaaa catgactgat atcaaagctg taatttttga ctggaacgga acgctcgttg
     3361 atgatgtttg gctcagtgtc aatgccatga acgccatgtt ggaaaaaagg ggaaaaaaac
     3421 cgataacaac tgaatattac aaatcaattt tttcatttcc ggttataaat tattataaag
     3481 aacttgggct tgatgttgaa aatgaatggg agataatttc cagagaattc atggatattt
     3541 atcttggagc atcagaaaaa ctcaaacttt tcagagatgt agaaccggtt gtaaaatatt
     3601 tcagggaact atcgttcact acagggatac tttcggcaat ggagcactcg atcctgaata
     3661 agcaccttga acacttcaga atatctcaac tctttgattt cgtacaggga atagaaaacc
     3721 attatgcggg cggcaagctt catctcggca gaaagatcct tgaaaagaca gggcttaaaa
     3781 gtgaagagat cctttttgtc ggtgacacat atcacgacta tgaagttgca aaagaaatgg
     3841 ggtgcaaagt cgtcctcata aaccgcgggc acaaccctta cgaaatgctc aaaagcacaa
     3901 atgcacccgt cctcaattca ctcgaagaac tgaaa
//
LOCUS       DAOOYB010000270         3920 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_27475_length_3920_cov_7.791721, whole genome shotgun sequence.
ACCESSION   DAOOYB010000270 DAOOYB010000000
VERSION     DAOOYB010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3920
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27475_length_3920_cov_7.791721"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            83..502
                     /locus_tag="PL056_11960"
     CDS             83..502
                     /locus_tag="PL056_11960"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HPM47777.1"
                     /translation="MKNRNDQIEIIRKKIIEELNPKLIYLFGSYAKHRENDESDIDVA
                     VLCEEKINPVKLFNLQLSLGQLVDRDVDLIDMSIIDIPFKIEIISARYPLFQISEESR
                     CKFEMNTISQYGRFNEEREIIFEKRLGLSGGELWKLF"
     gene            487..903
                     /locus_tag="PL056_11965"
     CDS             487..903
                     /locus_tag="PL056_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003348173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HPM47778.1"
                     /translation="MEVVLDKISSITRCVERIVEEYQDDETSIMNITKQDSIVLNLQR
                     ACELSIDIANYIISRNGLEVPKTTRESFAILERHEIIDGTLSDNLQKMVGFRNIAVHN
                     YKKLSLEILSEIIRNNLNDFEIYSEKIIEFMKGGRN"
     gene            937..1809
                     /locus_tag="PL056_11970"
     CDS             937..1809
                     /locus_tag="PL056_11970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47779.1"
                     /translation="MMSLGEIMEIGYEIPKIEDAVIFEKLIKEILNANNEIENVNING
                     RSGQKQDGVDIFYKKHGSWIGVQCKVRSTNKHFSKDELLSEIVKAKSFNPKLSKYYIY
                     TTLSRDAVTQRFVREIDDELCKSEEFSLEMLFWEEIKEILMKKECENVFFRYYNRFFK
                     DNLTIGQAIGKLVNLDLGFNEKHDTHYELILGTIPKNNGNGGNVDYYRGTYFIVNLNC
                     NKMEFFKKAFESGRAECFPSDIASAFDCEIDRYRISKWIESFENFDDFIYEDVRNCFF
                     SIDTEESLEFRKQL"
     gene            1831..3114
                     /locus_tag="PL056_11975"
     CDS             1831..3114
                     /locus_tag="PL056_11975"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HPM47780.1"
                     /translation="MRKIQATFFTILLMGFISCDNGQTFGVVTNGAEGEPCYEDGSCD
                     KGMICKSDICVKEEDISNSGKDDGENSYIDENNSDSDTSDDLKYCGDNIINDDEICDG
                     GTKNCTSISSEYTGGTANCRNDCSGWDVSTCEGTAAPFCGDNIINNNEICDGGTKACV
                     SISQDYSSGTAICKSDCSGWNVSNCKYCGNGSVDSGEICDGGTKSCTSISSDYVSGTA
                     NCRNDCSGWDVSKCDGTVAPFCGDNIVNNGEICDGGSKNCTLISSEYKSGVAICKADC
                     SDWNSDNCSSNCPVSNMFCHSFSGNNWSDASSDYMDWYEAKTYCEDIIGGRLPTISEL
                     RTLIQNCLATETGGECKATDDCLSWDDCWANSCYGCEYNDSGKYSVFGDTYWFWSSSP
                     RVDGSTDYIWSVSFRSGGVSYSKKDTSNGNVRCVN"
     gene            3252..3551
                     /locus_tag="PL056_11980"
     CDS             3252..3551
                     /locus_tag="PL056_11980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47781.1"
                     /translation="MIRIENDALELYKKICEKILLFENKKDVCLILSKKAVLMLKQMN
                     KFSDNCSSNDIFLNLIDYASRNGHSILKIQVVENTCNEELFEIKKCLERPKSKLS"
     gene            3750..>3920
                     /locus_tag="PL056_11985"
     CDS             3750..>3920
                     /locus_tag="PL056_11985"
                     /inference="COORDINATES: protein motif:HMM:NF014517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HPM47782.1"
                     /translation="MTGAESCTGGLITSALVDCPGVSAVLHESFVTYSNDSKIWLLGV
                     GSETLKNFGAVSI"
BASE COUNT         1368 a          522 c          850 g         1180 t
ORIGIN      
        1 gctcgacatg ataagaagag cagtttattt gaaataaatt attatatcgg ttaagatgac
       61 aggtcttaaa aaagagggac ttttgaaaaa ccggaatgat caaattgaaa taattcgcaa
      121 aaagataatt gaagaactga atccgaagct gatctatctc ttcgggtctt atgcaaaaca
      181 tcgggaaaat gatgaaagcg acattgatgt tgcagtgctt tgtgaagaaa agatcaatcc
      241 ggtaaaactg tttaatttgc agctttcatt gggtcagctt gttgatagag atgttgatct
      301 gattgatatg tctatcattg atattccgtt taaaatagaa ataatatcag cccgttatcc
      361 tctttttcaa ataagtgagg aatcaagatg taagtttgaa atgaatacaa tttcgcaata
      421 tggcagattc aatgaagaac gggaaataat atttgaaaaa cggctgggac tttccggagg
      481 tgagttatgg aagttgtttt agataagatc agctcgatca caaggtgtgt cgaaagaatt
      541 gttgaagaat atcaagatga tgaaaccagc ataatgaaca taaccaagca ggattccata
      601 gtgctgaatc ttcagagagc gtgcgaactt tcgattgaca ttgctaatta tataatttcg
      661 cggaatgggc ttgaagtgcc taaaacaaca agagaatctt ttgcaattct tgaaaggcat
      721 gaaataattg acgggacttt gtcggacaat cttcagaaaa tggtcggttt cagaaacatt
      781 gccgttcaca actataaaaa actttccctt gaaatccttt cagaaattat ccgcaacaac
      841 cttaatgatt ttgagatcta tagcgaaaag atcatcgaat tcatgaaagg cggacggaat
      901 taagagagtt gtaatcatct tgcggagagg gaaaatatga tgagtctcgg tgaaataatg
      961 gaaatcggtt atgaaattcc aaaaattgaa gatgctgtta tttttgaaaa actgattaaa
     1021 gaaatcttga atgccaacaa tgagattgaa aatgttaata taaatggtcg gtctggacaa
     1081 aagcaggatg gtgtagatat attttataaa aaacacggga gttggatagg agttcaatgt
     1141 aaggtaaggt caactaataa acatttttca aaagatgagt tgctgtcaga aattgtaaaa
     1201 gcaaaaagct ttaatccaaa actttctaaa tattacatat atacgacttt aagtagagat
     1261 gctgtaacac aaagatttgt tagagaaata gacgatgagc tgtgcaaatc tgaggaattt
     1321 tctttggaaa tgcttttttg ggaagaaata aaagagattc taatgaaaaa agaatgtgaa
     1381 aatgtgtttt tcagatacta caatcggttt tttaaggata atttaacgat tggtcaagca
     1441 ataggtaagt tggttaatct tgatcttggt tttaatgaaa aacatgatac gcactatgaa
     1501 ctaattttgg gaacaattcc taaaaacaat ggtaatggag gaaatgttga ttattaccga
     1561 ggaacatact tcatcgttaa tttgaactgc aataaaatgg aatttttcaa aaaagcgttt
     1621 gaatctggca gagcagaatg ctttccttcc gacatcgcca gcgcttttga ttgtgaaatt
     1681 gatagatata gaatcagtaa atggattgaa agttttgaaa attttgatga ttttatttat
     1741 gaagatgtta ggaattgctt tttttcaata gatacagaag aaagcctaga atttagaaaa
     1801 caactataag aactttagga gaaagggatg atgagaaaaa ttcaagcgac attcttcaca
     1861 atattattaa tgggttttat ttcttgcgat aatggtcaaa catttggagt tgttacaaat
     1921 ggcgcagaag gtgagccatg ctatgaggat ggttcttgtg ataaaggaat gatttgcaaa
     1981 tcagatattt gtgttaaaga agaagatatc agtaactcag gtaaggatga tggagaaaat
     2041 tcatatattg atgaaaacaa tagtgattct gatacttctg acgatttaaa atactgtgga
     2101 gataatataa taaatgatga tgaaatctgt gatggtggca caaaaaactg cacttcaata
     2161 tcatcagaat atacaggtgg cacagcaaat tgcaggaatg attgctccgg ctgggatgtg
     2221 agcacatgcg aaggaacagc agctccgttt tgtggtgaca atattatcaa taacaatgag
     2281 atctgtgacg gtgggacaaa agcgtgtgtt tcgatatctc aagattattc aagcggcaca
     2341 gcaatatgta aaagtgattg ttcaggatgg aatgtaagca attgtaaata ttgtggaaac
     2401 ggtagtgttg attcaggtga gatttgtgat ggaggaacaa aatcctgtac ttccatatct
     2461 tcagattatg tatctggcac agcaaattgc agaaatgatt gctccggctg ggatgtgagt
     2521 aaatgtgacg ggacagtggc tccgttttgc ggtgataata ttgtaaataa cggtgaaatc
     2581 tgtgatggtg gaagtaaaaa ctgtacatta atatcatcag aatacaagag cggagtcgct
     2641 atatgtaaag ctgattgttc cgattggaat tcagacaatt gcagcagcaa ttgccctgtt
     2701 tctaatatgt tttgtcactc tttttcagga aataattggt cagatgcctc aagtgattat
     2761 atggattggt atgaagcaaa aacttattgt gaagatataa taggaggtcg gttaccaaca
     2821 atatcagaac ttagaacgct tattcaaaac tgtcttgcca ccgaaactgg tggtgaatgc
     2881 aaagccactg atgactgttt gtcatgggat gattgttggg ctaattcatg ttatggatgt
     2941 gaatataatg actcgggtaa atattctgtt tttggagata cttattggtt ctggtcatct
     3001 tcgccccgtg ttgatggtag tactgattat atctggtcag tgtctttcag atccgggggt
     3061 gtgagctaca gtaagaagga cacctccaac ggcaatgtcc gctgtgtaaa ttaatgaaaa
     3121 aagatttgtt gcgggaagta tccagttcgt cccactttgg cttgggcagt cactttttat
     3181 tatggaagtg ttggaggaca cagtaaaact tatgaaacaa atataaggtg cgtgaaataa
     3241 aggagtttga tatgattaga attgaaaatg atgctttaga gttatacaag aaaatttgtg
     3301 aaaaaatcct cctatttgaa aacaaaaaag atgtttgcct gatactttca aaaaaggctg
     3361 ttctgatgct aaagcaaatg aacaaatttt ctgataattg ttcttcaaat gacatatttc
     3421 tgaacctcat tgattatgca tcgagaaatg ggcactcaat tttaaaaatt caagttgttg
     3481 agaatacatg taatgaggaa ttatttgaaa taaaaaaatg cttagaacga ccaaaatcga
     3541 aattgtcata gaagaatagg acgcaatata taatatgttt gtcgcagaat aagttgtcac
     3601 ttgaagaaga ttataagaac atggaagaaa tgttttttgt taaaccgata acttttcctg
     3661 aaatactgga cattctttca ttatgggaaa aagagtttaa tgaataaata taagttgttg
     3721 taaaaaaact gatcgaaaaa aagctaatta tcacgggagc cgaatcgtgc acaggcggat
     3781 tgataacttc agccctcgtt gactgccccg gagtgtccgc agtattgcat gaaagttttg
     3841 taacatattc aaatgattca aaaatatggc ttcttggcgt tggctcagaa actttgaaaa
     3901 attttggtgc cgtaagcatt
//
LOCUS       DAOOYB010000271         3881 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_27850_length_3881_cov_9.835337, whole genome shotgun sequence.
ACCESSION   DAOOYB010000271 DAOOYB010000000
VERSION     DAOOYB010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3881
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27850_length_3881_cov_9.835337"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1346)
                     /locus_tag="PL056_11990"
     CDS             complement(<1..1346)
                     /locus_tag="PL056_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HPM47783.1"
                     /translation="MTEKMMVLIFQIAILVIAAKIFGIIFAKLKIPSVLGELFAGIIF
                     GPYLLGSIAVPGFPHGFFPMDPTALMPVSGELYGMATFASIILLFLTGLETDIEMFLR
                     YSFAGSLVGIGGVVASFMVGALTGMYFLGLPFMSPAIIFLGVMSTATSVGITARILSE
                     KRALDSPEGVTTLAGAVIDDILGIILLAIAIGIAGAMMHSSGEEIQWGSISLIAVKAI
                     AVWILFTAIGLLFAERIANLLKKFKDKSLISIFSLGLALIVSGIFEQQGLAMIVGAYV
                     TGLSLSKTDISYVIQETLHPLQMFFIPLFFAVSGMMVDLRVFLSKEILAFGLIYSAGA
                     ILAKFVGCGIPALFMGFNKLGASRIGIGMIPRGEVALIIAGIGLANGILDQKIFGVSI
                     MMTLITTLVSPPILSAVFSIKKRGTRKTPVTGDTVETEYSFDSELITVALVRQIRD"
     gene            complement(1536..1865)
                     /locus_tag="PL056_11995"
     CDS             complement(1536..1865)
                     /locus_tag="PL056_11995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47784.1"
                     /translation="MLKFSRKCSRCGVVEVAEKCYERHFISSFNEVLCDTCVENLLEK
                     GQISAVTDAEGNIAYKQNLENIYKRIIEIEEENGALEQKIYQNKRMLRRLERIKDFVE
                     MSSTERD"
     gene            1991..3652
                     /locus_tag="PL056_12000"
     CDS             1991..3652
                     /locus_tag="PL056_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013450378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HPM47785.1"
                     /translation="MAENFITDIIDSDLRSGKHSAIVTRFPPEPNGYLHIGHAKSICL
                     NFGIAKQYGGKTHLRFDDTNPEKEEVEYVESIKNDVKWLGFDWGDNLFYASSYFGQLY
                     DWAVEMIRNGKAFVCSQTLEEMRRDRGTAKGAGVESPDRNRSIEENLDLFERMKNGEF
                     KENQYMLRAKIDMAHPNVLLRDPVMYRIRHAEHHNTGNEWCIYPTYDYAHGQSDAIEG
                     ITHSICTLEFESHRPLYEWFLEAIGIEKRPQQIEFARLNLTNTVMSKRLLMQLVKEHH
                     VNGWDDPRMPTIAGVRRRGYTPESIRDFAERIGVAKRESIVSLDLLEHCVREDLNKKV
                     PRFMGVIDPVKVVITNYPDDLTEEFEAVNNPEDENSGKRLVPFSKVIYIEREDFEEFP
                     PPKFHRLAPGNEVRLRSAYYVTCTEVVKDISGRIVEIHCNYDPATKGGWSSDGRKVKG
                     TIHWVSATNSLKIEARLYENLFTKEDPYDCEEGKTFLDNINPESLKVVEAIAEINLKN
                     LPSGTQFQFERVGYFCIDPDSTDEKIVVNRIVTLKDKWARIQKSQ"
BASE COUNT         1218 a          811 c          835 g         1017 t
ORIGIN      
        1 taatctctta tctgccttac aagagcgacg gtgataagtt ctgaatcaaa cgagtattcc
       61 gtttcaacgg tatctccggt gacaggtgtt ttcctggtgc cccgtttttt gatcgaaaag
      121 actgcgctca ggatcggcgg cgacacaaga gtcgttatga gagtcatcat gatgctgaca
      181 ccgaatattt tctgatcaag tattccgttg gcaagtccga tgcctgcaat tatgagggcg
      241 acttcacctc tagggatcat tccaatacct attctggaag ctccgagctt gttaaatccc
      301 atgaaaagtg cgggtattcc gcaacccaca aattttgcca gtatcgcacc ggccgaatag
      361 atcagtccga aagccaggat ctcttttgaa agaaaaacac gaagatcgac catcatgccg
      421 cttaccgcaa agaaaagagg tatgaagaac atctgcagag gatgaagggt ttcctgaatg
      481 acatagctta tatcggtctt tgaaagggaa agaccagtta cataggctcc tacgatcatt
      541 gcaagaccct gctgttcaaa aattcccgac actatcaggg caagcccgag agaaaatatc
      601 gaaataagac ttttgtcctt gaattttttt agaagatttg caattctttc ggcaaaaaga
      661 agtccgatag cagtaaaaag gatccacaca gcgatcgctt tgacagctat cagtgatatt
      721 gagccccact gtatctcttc acccgaactg tgcatcattg ctccggcaat acctatggct
      781 attgcaagaa gtattatgcc gagaatgtca tcaattacag cccctgcaag cgttgtaaca
      841 ccctcaggag agtcaagagc tcttttttct gaaagaatac gggctgttat accgacagaa
      901 gtggccgtgc tcattacccc cagaaaaatt atagcaggag acatgaaagg aagtccgaga
      961 aaatacattc cggtcaaagc gccgaccatg aacgaagcga caacacctcc tattccgaca
     1021 agacttcccg caaatgaata gcggaggaac atctcaatgt cagtttccag acctgtaaga
     1081 aaaagaagta tgatggaagc gaaagtcgcc attccgtaaa gttctcctga aaccggcatg
     1141 agcgctgtcg gatccatcgg gaaaaatcca tgcggaaaac cgggaacggc aatacttcca
     1201 agaagatacg gtccgaatat tattcctgcg aacaactctc cgagaactga aggtatttta
     1261 agtttcgcaa atataattcc gaagatcttt gcggcaatga ccagaatggc tatctgaaat
     1321 ataagcacca tcatcttttc ggtcatttga tctcccccgc caagttctga tccggataga
     1381 gggaactgaa ggaagaatgc gtagagcaca aacaggattc gagttgcata tctcattttt
     1441 gtctccttaa taagaaacac caacggggac tcaagctact caggtattat taaatttcaa
     1501 gcaaaattta aatgataaag ggaatttaag aaagatcagt ccctttcggt tgagctcatt
     1561 tcaacgaaat ccttaattct ttcaagccgt ctgagcattc tcttgttctg atatatcttc
     1621 tgttccagag cgccgttctc ttcttctatc tctatgatcc ttttgtagat gttctcaaga
     1681 ttctgtttgt aggcgatgtt cccttcggca tcggtaacag ctgaaatctg tcctttttcc
     1741 agaaggtttt caacgcaagt atcacagaga acttcgttga atgaacttat aaaatgtctt
     1801 tcatagcatt tttcagcaac ttcaacaact ccgcatcttg aacattttct cgaaaattta
     1861 agcattgctt tcctccacat attgaataaa taaaaactgt attattttat gcaaaacatt
     1921 atatttgtaa agcttttaat aggccggctg ccggtaaaag ctttaaaaca atgttttttt
     1981 gaggtggatc atggctgaaa attttattac tgacatcatt gattcagatc ttcgaagcgg
     2041 aaagcactct gcgatcgtta cgaggttccc ccctgaaccg aacgggtatc ttcacatcgg
     2101 tcatgcaaaa tcgatatgcc tcaatttcgg gatcgcaaag cagtacggcg gaaaaacaca
     2161 tctccgtttt gatgatacaa accctgaaaa agaggaagtt gaatatgtcg aatcaataaa
     2221 aaacgatgtg aaatggctcg gttttgactg gggtgacaat cttttttatg catcaagcta
     2281 ttttggtcag ctttatgact gggcagttga aatgatcaga aacggcaaag catttgtctg
     2341 ttcccagact cttgaagaga tgagaagaga ccgcggaacg gcaaaaggtg cgggtgtgga
     2401 aagtcccgac agaaaccgtt caattgaaga aaatctcgat ctttttgaaa gaatgaaaaa
     2461 cggtgagttt aaggaaaatc agtatatgct cagggcgaaa attgacatgg ctcaccccaa
     2521 tgttctgctc agagaccctg tcatgtacag aataagacac gccgaacatc acaacaccgg
     2581 aaacgaatgg tgcatatatc cgacttatga ctatgcccac ggtcagagcg atgcgataga
     2641 agggataacc cactcgatat gtacgctgga attcgagtcg cacagacctc tttatgagtg
     2701 gttccttgaa gcgatcggaa ttgaaaaaag acctcagcag atagaattcg cccgtcttaa
     2761 tcttacaaac acagtgatga gcaaacggct tctgatgcag cttgtgaaag agcaccatgt
     2821 aaacggctgg gacgatccca gaatgccgac aatcgcagga gtcagaagaa ggggatatac
     2881 acctgaatcc attagagatt ttgctgaaag gatcggtgtt gccaaaagag agagcatcgt
     2941 gtcgttagat ctgcttgaac attgcgtgag ggaagatctc aataaaaaag tgccccgctt
     3001 catgggggta atagacccgg taaaagttgt gataaccaac tatcctgatg atttgactga
     3061 agaatttgaa gcagtcaata atcctgaaga tgaaaattca ggaaaacggc ttgtcccttt
     3121 cagcaaagtt atctatatcg aacgggagga ttttgaagag ttcccgccac ccaaatttca
     3181 ccgtcttgct ccggggaacg aggtgcgtct gagatccgcc tattatgtca catgcacaga
     3241 agtcgtgaaa gatatatcgg gcagaattgt cgagatacac tgcaattatg accctgccac
     3301 aaaaggtgga tggtcaagtg acggcagaaa ggtcaaaggg acaattcact gggtaagtgc
     3361 cacaaattcc ttgaaaattg aagcacggct ttatgaaaat ctattcacca aggaagatcc
     3421 gtatgattgt gaagaaggga aaaccttcct tgacaacatc aatcccgaat cgcttaaagt
     3481 tgttgaagca attgccgaaa tcaatctgaa aaaccttcca tcaggaacac aattccagtt
     3541 tgaaagagtc ggctatttct gcatagatcc tgattcaacg gatgaaaaaa ttgttgtcaa
     3601 ccgcatagtc acgctcaagg ataagtgggc tagaatacag aaatcacagt aaatacccga
     3661 cacagaaagt taaacaaaaa tagttcttgt tttagttttc aacagtaata taatctttta
     3721 ttgtgaatgg tgttgatgag atttttttaa atagcggggg cgtttaatga aaaaaacaat
     3781 tttaagcttt tttgcgattt tatttctttt tgtccttttt acaggttgcg aaaattccaa
     3841 aagcaaaccg aatcaggacg atgaacagaa cgatgactca c
//
LOCUS       DAOOYB010000272         3855 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_28106_length_3855_cov_8.581842, whole genome shotgun sequence.
ACCESSION   DAOOYB010000272 DAOOYB010000000
VERSION     DAOOYB010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3855
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28106_length_3855_cov_8.581842"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="PL056_12005"
     CDS             <1..189
                     /locus_tag="PL056_12005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47786.1"
                     /translation="KRNTAPVVIGNVFHKALEMYFKKAGVPRRYQVEFALKEYYKDKA
                     PEIFMYINEKTNNNGGTK"
     gene            186..497
                     /locus_tag="PL056_12010"
     CDS             186..497
                     /locus_tag="PL056_12010"
                     /inference="COORDINATES: protein motif:HMM:NF016820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HPM47787.1"
                     /translation="MRSIMKRENLVITAYLAVFAALIVFFPDSLHAANPFEKIVGKFE
                     NVKDTVFGVAKILAVIGIIVGGIKKVLGHPDSWTWLWSSGLGAFIIFSADAIVTWLAE
                     S"
     gene            498..782
                     /locus_tag="PL056_12015"
     CDS             498..782
                     /locus_tag="PL056_12015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47788.1"
                     /translation="MKVWKVLSNSNGSKSGNVDRYTQYSLAGVVLPLLFLTNSFIAAI
                     FGIVAVFMISYFAKRYFNGRADWLFIWYRLLFKPQGSYNLNERDNAAKRK"
     gene            763..3111
                     /locus_tag="PL056_12020"
     CDS             763..3111
                     /locus_tag="PL056_12020"
                     /inference="COORDINATES: protein motif:HMM:NF040179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM47789.1"
                     /translation="MQQKESKIQKKTIFDELRYLSIENDYILNSVYQRYEVYEVTGLD
                     CYFKDQNLSAAVNFFNTELSDTDVSIEFFSLSKLKDERLTERSYVDYILKDRVNYLDR
                     TLQVKRLSKFICITTDKFSKSFPDTEIKSKVHESFFSILGTGFRKLTTPEIKKLLYGI
                     ISMDDEFRVSPSMSQREQLIQSQIRYYPEFFQIGNQYLSVLTLKNLPEEIFYFDLDYI
                     LDFFQFEYMFVSAVTVPNQQKEKTRLTVLRNTAKSAKYKSHNVDHEAEQKYEEAETLK
                     GLITKEGHSIVFSTQKIILWHKDINTLRQTVEYVQNVFKKREIFFFPEELFHDKEFFR
                     SLPAQTILSERGNRVLSPNALALFPLSRIAQGDINQPFPLFLRTTTGQLYGFDAGSDL
                     RANWNASVYGASGSGKSVTMNMIIAFTMYLRIKAEGGKIFIIDFAGAENSSYRKMAQL
                     FGGSFIPIDSSGKYSINPFPARNQILIDGEFDASQLTFLSIVVDMIVGNTGDGTGENL
                     KRGIISKAIVQMYREKEFPSLKDLPHYLADVDSDDKRLKDELFKLLSDFLDASNPASK
                     IIAGNSNIRYSDNPFVIFDLQGINGLTDRMKQLLTFIVIQEAKKTAFKTPGFKFLIMD
                     ECAQLIKQPAMADLVEEMFATARKYRTGVWTVTQNFLSFKETNLSSKIKINTTTTIFL
                     THAEDPEAKRLVAADFGFTQQQKEAFESLRMEKGKYSLALFLTTSESGQESAVVRMEL
                     SPFGYQIAQSDRKQNDFIESVSKKHNVSIIRACQMIANGEVK"
     gene            3108..>3855
                     /locus_tag="PL056_12025"
     CDS             3108..>3855
                     /locus_tag="PL056_12025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47790.1"
                     /translation="MKRLILLLFTVLAPVILTADETFQPGVAAGKILGNPSVTALMDR
                     GWHFILIIFAFVLAKNMFTFVSGGGTPNWFLIIGKFMIIFFLYNHAIFVVTTFTNEVV
                     VATVISDSERLSKAFSDLDVALVTLAMGDPDELQKAKDAAKVGPLPDAVTDTFVEMGR
                     LFSFQMLLTVVFSVVMKGLLTVSLITKMLMIDIFWPIFFQLVVVGFVFAVPFAALDGG
                     MDSLKKFAINVVEVAMWPVIYNIAFGLTTGS"
BASE COUNT         1108 a          890 c          813 g         1044 t
ORIGIN      
        1 aaaagaaata cggctccggt ggttatcggc aatgtatttc acaaggcact tgaaatgtat
       61 tttaaaaaag cgggagtacc cagacggtat caggttgaat tcgcattgaa agaatattac
      121 aaagacaaag cacctgaaat attcatgtac atcaacgaga aaaccaacaa caacggagga
      181 acgaaatgag atccatcatg aaaagagaaa accttgtcat caccgcttac cttgcggttt
      241 ttgcggcact tattgtattt tttcccgact cgctccatgc ggcgaaccct tttgaaaaga
      301 tcgtaggaaa atttgagaat gtcaaagata ccgttttcgg tgttgcgaag atccttgccg
      361 ttatcggtat aattgtcggc ggtatcaaaa aagttctcgg acatcccgat tcatggacat
      421 ggctgtggtc aagcggactc ggcgccttta tcatcttttc cgctgatgcc attgtcacat
      481 ggcttgctga aagctaaatg aaggtctgga aagttctctc gaacagtaac ggttccaaat
      541 caggaaatgt tgaccgttac acccaatatt cactggcagg ggtagtttta cccctgctct
      601 tccttacgaa cagctttatt gcggcaattt tcggaattgt tgcggttttt atgataagtt
      661 actttgcaaa gagatatttc aacggacggg cggactggct tttcatctgg taccgcctgc
      721 ttttcaaacc gcagggcagt tacaatctta atgagagaga caatgcagca aaaagaaagt
      781 aaaatacaga agaaaaccat atttgatgaa ctcagatacc tttcaattga gaacgactac
      841 atccttaatt ccgtatatca gcggtatgaa gtttacgaag tcacgggtct tgactgctat
      901 ttcaaagatc agaacctgtc cgccgccgtc aatttcttca ataccgaact ctcggacact
      961 gatgtttcca tcgagttctt ctcactttca aagctgaaag atgaacggct tacggaacgg
     1021 tcctatgtgg attacattct taaagaccgt gtgaactacc ttgaccgtac acttcaggtg
     1081 aaaagacttt caaaattcat ctgcataact acagataaat tctcaaagtc attcccggac
     1141 actgaaataa agtcaaaagt tcatgaaagc tttttcagca tcctcggtac agggttcaga
     1201 aagcttacga ccccggaaat aaagaaactc ctttacggca ttatctcaat ggacgatgaa
     1261 ttcagggtat ccccttcaat gtctcagagg gaacagctca tacagtcaca gatccgttat
     1321 tatcctgaat ttttccagat agggaatcag tacctttcgg tgcttacgct taaaaacctc
     1381 cctgaagaga tattttactt tgaccttgat tacattctgg atttcttcca gtttgaatat
     1441 atgttcgttt ccgctgtcac agtaccgaat cagcagaagg aaaagacacg gctcaccgtg
     1501 ttaaggaaca ctgcaaaatc ggctaaatac aaatcccata atgtggatca cgaagcggaa
     1561 cagaaatatg aggaagccga aacgctcaag ggactcataa caaaggaagg tcattcaata
     1621 gttttctcaa cacagaaaat tatactctgg cataaagaca tcaatacatt aagacagacc
     1681 gttgaatatg ttcagaatgt ttttaagaaa agggagatat tcttttttcc ggaagaactc
     1741 tttcacgata aggagttttt ccgctcactc cccgcccaga caatactttc agaacgggga
     1801 aaccgtgttc tctccccgaa cgctctcgcc ctgttccctc tttcgaggat agcacagggc
     1861 gacataaatc agccctttcc gctgttcctg cggacaacga cgggacagct ttacggtttt
     1921 gatgccggga gtgatctccg tgcaaactgg aacgcttcag tttacggtgc ttcaggttcg
     1981 ggaaaatcgg tcacgatgaa catgataatc gctttcacga tgtatctccg cataaaagcc
     2041 gaagggggaa aaatattcat cattgacttt gcgggagccg aaaactcttc ataccgtaaa
     2101 atggcacagc ttttcggcgg aagttttatc ccgatagatt cctccggaaa atactcaata
     2161 aaccccttcc ccgccagaaa ccagatactt attgacggtg aattcgatgc ttcacaattg
     2221 accttcctgt caattgtcgt tgatatgatc gtcggtaaca caggggacgg cacaggcgaa
     2281 aacctcaaga gaggtattat ttcaaaggcg atagtccaga tgtaccggga aaaggaattc
     2341 ccctcactta aggatctccc ccactacctt gccgatgtcg attcagatga taaacggctt
     2401 aaagacgagc ttttcaagct cctcagtgac tttcttgatg ccagcaaccc cgcttcaaag
     2461 atcattgccg gaaattcaaa catccggtat tctgacaatc cctttgtaat cttcgatctt
     2521 cagggaataa acggactcac tgaccgtatg aaacagcttc tcaccttcat agtcattcag
     2581 gaagctaaaa agaccgcatt taaaactccc ggattcaaat tcctcataat ggatgaatgt
     2641 gctcagctca ttaaacagcc cgcaatggcc gatctagttg aggaaatgtt cgccaccgcc
     2701 cgtaaatacc gtaccggtgt ctggacagtt acacagaatt tcctttcatt caaggaaacg
     2761 aacctttcct caaagataaa gataaatacc actactacta ttttcctgac ccacgccgaa
     2821 gaccccgagg cgaaacggct tgtcgccgct gatttcggat ttacacagca gcagaaggag
     2881 gcctttgaat cgctccgcat ggaaaaggga aagtattctc ttgcactctt cctcaccact
     2941 tcggaaagcg gtcaggaatc ggcagttgtc agaatggaac tttccccttt cggctaccag
     3001 atagcacaga gcgaccggaa acagaacgat tttattgaat cggtatcgaa aaaacacaat
     3061 gtctcaataa tcagggcatg tcagatgatc gccaacggag aggtaaaatg aaaagactga
     3121 tattactgct ttttacggta cttgcacccg tgatactgac agctgacgaa acctttcagc
     3181 ccggagtggc cgcaggaaag atcctcggta atccgtccgt taccgccctt atggatcgcg
     3241 gatggcattt tatactgata atattcgcct ttgtcctcgc aaagaacatg ttcacatttg
     3301 tttcgggagg cggtacgccc aactggttcc ttataatagg aaaatttatg ataatatttt
     3361 ttctttataa tcatgctatc tttgttgtga cgactttcac caatgaggtt gttgtggcaa
     3421 ccgtcatttc agattcagaa cggctttcaa aggcattttc agaccttgat gtcgcacttg
     3481 taacccttgc catgggcgac cccgatgaac ttcagaaggc aaaagatgcg gcgaaagtag
     3541 gcccgcttcc cgatgcagtc accgacacat tcgttgaaat gggtcggctg ttcagttttc
     3601 agatgcttct aaccgttgtt ttttcagttg taatgaaggg acttctcacg gtttcgctga
     3661 taacgaaaat gctgatgatt gacattttct ggccgatatt ctttcagctt gtcgttgtag
     3721 gtttcgtctt tgccgtgccg ttcgctgctc ttgacggcgg gatggattca ctcaagaaat
     3781 ttgcaataaa tgtcgttgaa gtcgcaatgt ggcctgtcat ttataacatt gctttcggac
     3841 tcactaccgg ttcaa
//
LOCUS       DAOOYB010000273         3847 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_28204_length_3847_cov_15.516086, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000273 DAOOYB010000000
VERSION     DAOOYB010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3847
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28204_length_3847_cov_15.516086"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..943)
                     /locus_tag="PL056_12030"
     CDS             complement(<1..943)
                     /locus_tag="PL056_12030"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HPM47791.1"
                     /translation="MLNKIRKLKLFLIQLLKKSRTLRVLWETGYGFYVDDGFTFAAAI
                     SFYFMLSFIPFLILLGATTGYVIDYVQNIQNLTNEEMAVYIMNYLNAAIPFLSEKYVS
                     GFINIAEYKTSLTTIGLISLAVSATLLFSTLHYCFFKIFGGKSVNFILTRLMGIVFLL
                     TITILLFFLHYVTTIFISVTGFITTKFPSTAWIFEIVSDTRIYGFALSTVIIIFLFGI
                     LIYYFTYGVKRSKMAILYGALLFSFLWNGAKLVYNFYITEISNFSLFYGSATWIITSV
                     LWVYYSALILLLSMEFIKALIKVFPSLIDKEVPAKVKV"
     gene            complement(933..2306)
                     /gene="der"
                     /locus_tag="PL056_12035"
     CDS             complement(933..2306)
                     /gene="der"
                     /locus_tag="PL056_12035"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HPM47792.1"
                     /translation="MTSNMANNINPVIAIIGRPNVGKSTLFNRILRKNFAIIDDMPGV
                     TRDLNYQDLTYIDKPFTIIDTGGFHIDEEGMDAITKGIKDQINEVLETVDGVILLCDA
                     EAGLIESDREIYNLIRKKGIRSWLAVSKIDSENREMLIGEFYETGADMVYPVSGKTGY
                     GVNDFLDEMTKDFWTEKEIEEKAPPREKEIKIAIIGRPNVGKSTLVNSILGEEKMLVS
                     PIPGTTMDPIDSFCTYKDKKLKIIDTAGVRRKKKIEMLMEKAAIVRAFRAIDRSDVVI
                     FMMDANELATDQDLRILGLAHEKGKASIVVINKWDTMEKETGTYEKIVKDLRDRIKFT
                     PYAPVISIAAINGMRVNKLLDKVLEIYDNYFKSVEKPALEEWLQVCLRRNPPPLTRKN
                     KIIHFNSIEMVEVAPPTVVIKVSSPESVHFSYDRYLLNRFYESFPYYEGVPIKLVYRR
                     GFKKIAK"
     gene            2520..3824
                     /locus_tag="PL056_12040"
     CDS             2520..3824
                     /locus_tag="PL056_12040"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPM47793.1"
                     /translation="MKRNISKKLIAWKDSSSRKPLIIRGARQVGKTYTVKEFGSEHFS
                     NVVVLDFERDRSLNAVFEKDLDPKKLLIELEIHTGSRIIPGETLLFFDEIQACERALL
                     SLRYFYEEMPELHIIAAGSLLEFAMSNISFPVGRVTFEWMRPMTFREFLIACSKDIAA
                     QRIPSIFSSEPVLETLHNLLIEQLRLYFIVGGMPEAVKRFIETGSAASVKEVHDDIIH
                     SYIQSLAKYNSKVNIESIEQILRVIPSKVGSQIKYTRLDPDRRIEVTKTSLNILEKAL
                     LVNLIHSSSVSGLPLGAESSSKIFKPLFLDIGLMQSVCGIDPKESITATDLNNVYKGA
                     IAEQFVGQELLAAGGSENHKLYYWNRSKKSSTAEVDFVFVRNSTIYPVEVKSGAKGRM
                     KSMHLFLEEHPEIKKGFVLSSTFSQHQLVENIVFAPIYSELG"
BASE COUNT         1152 a          784 c          745 g         1166 t
ORIGIN      
        1 agaccttgac tttggcagga acttctttgt cgatcagtga cgggaaaacc ttgatgagtg
       61 ctttgatgaa ttccattgaa agaagcagta tcagagcgct gtaataaacc cacagaacgc
      121 ttgttattat ccatgtcgct gaaccgtaaa acaaagaaaa gttagaaatt tcagtaatat
      181 agaaattata cacgagtttt gcaccattcc agagaaaact gaaaagaagc gccccgtaaa
      241 gaatcgccat ttttgatctt ttcactccat atgtgaagta atatatgagg attccgaaaa
      301 gaaagatgat tatgactgtt gaaagggcaa atccatatat cctggtgtct gaaacaattt
      361 caaatatcca tgcggtagag ggaaattttg ttgtaatgaa tccggttacc gaaatgaaaa
      421 tggtggtcac atagtgcaga aaaaacaaca ggatggttat tgtaagaaga aaaactatcc
      481 ccataagacg ggttaaaatg aaattgaccg atttcccgcc gaagatcttg aaaaaacaat
      541 agtgaagtgt cgaaaaaaga agagtcgctg aaacagccag agagataaga ccgatcgttg
      601 taagactggt tttgtattct gcgatattga tgaaccccga aacatatttt tcacttaaaa
      661 aagggattgc ggcattcaga tagttcatga tatatacagc catttcctca tttgtgaggt
      721 tctgaatatt ctgaacataa tcaattacat atccagtagt tgctcccaga agtattagaa
      781 acgggatgaa gctcagcata aaatagaaac tgatcgctgc cgcaaaagtg aacccgtcat
      841 caacataaaa cccgtatccg gtctcccaga gcacccgcaa tgttctgctt tttttaagaa
      901 gctgaattaa gaaaagtttt aacttcctga tcttatttag caattttctt gaaacctctt
      961 ctgtagacaa gctttatagg aacaccttca taatatggaa acgactcgta aaatctgttg
     1021 agaagatatc tgtcatacga aaaatggacc gattcagggg aactcacttt tataacaacc
     1081 gtcggagggg ccacttcaac catttcaatc gaattgaaat gtataatttt attttttctg
     1141 gtcaatggag gcgggtttct tctcaggcag acctgaagcc actcttcaag agctggtttt
     1201 tctactgatt tgaagtagtt gtcataaatt tcaagaactt tgtcaagcag tttattaacc
     1261 ctcattccgt ttattgctgc aattgaaatg acaggtgcgt aaggggtaaa ttttatcctg
     1321 tcgcggagat ctttaactat tttttcgtat gttccggtct ctttttccat tgtatcccat
     1381 ttatttatta caactatcga tgcctttcct ttttcgtggg ctaaccctag aattctcaaa
     1441 tcctgatcag ttgcaagttc gtttgcgtcc atcatgaaaa ttacaacatc acttctgtcg
     1501 atcgccctga aagccctcac tatcgccgct ttttccataa gcatttcgat ctttttcttt
     1561 cttctgacac cggctgtatc aattattttc agctttttat ccttgtatgt gcagaaggaa
     1621 tcgatcgggt ccatcgtcgt tccgggaatg gggctcacaa gcattttctc ttcaccgaga
     1681 attgagttca caagtgtcga ttttcctaca ttcggtctgc ctattatcgc gatctttatc
     1741 tctttttccc ttggcggggc cttttcttcg atctcttttt cagtccagaa atcttttgtc
     1801 atttcgtcaa gaaagtcgtt cacgccgtaa ccggtctttc cggaaacggg atagaccata
     1861 tcagcaccgg tctcataaaa ttcaccgata agcatttccc tgttttccga atctatcttg
     1921 gaaactgcaa gccacgaacg gatccctttt tttctgatga gattgtatat ttccctgtct
     1981 gattcaataa gtcccgcctc ggcgtcacaa agcaggatca ctccgtcaac agtttcaagc
     2041 acttcattta tctggtcttt tatccctttc gttattgcgt ccattccttc ttcatcaatg
     2101 tgaaaaccgc ctgtatcgat gatggtgaag ggcttgtcga tatatgtaag gtcctgataa
     2161 ttcaggtctc ttgtcacacc cggcatgtcg tcgattattg caaagttctt cctgagtatt
     2221 ctgttgaaca gagtgctttt ccctacattc ggacgaccga ttatcgcaat aaccggattt
     2281 atgttgtttg ccatgttgct tgtcattttt tttccacctt tcagtcctgc aaaagttact
     2341 acctggctaa ggtactcaaa taactgaaaa acgcaacatc tgaaactgtg ccgggcgaaa
     2401 gcttctgtca aaaaaccttg cgaaattaca aaaaagtgta aaaataacct tgtgaaatta
     2461 caaaaaatat gtaaaaataa ccttgtaaaa ttacaaacct cttcacagtg aggtgattta
     2521 tgaaaaggaa tatatcaaag aaattaatag cttggaaaga ttcatcatca agaaagccgc
     2581 tgataatcag aggggcacgg caggttggga aaacatatac agtaaaagaa ttcggttctg
     2641 aacacttttc aaatgttgtg gtgcttgatt ttgaaaggga cagatctctt aatgctgttt
     2701 ttgaaaagga tcttgatccc aaaaaacttc tcattgaact tgaaattcat accggttcca
     2761 gaataattcc aggcgaaaca cttctttttt ttgatgaaat tcaggcatgt gaaagagctt
     2821 tgctgagctt aagatacttt tatgaagaga tgccggagct ccacataatt gcggctggtt
     2881 cgcttcttga atttgcaatg agcaatattt catttcctgt cggcagagtg acatttgaat
     2941 ggatgcgccc gatgacattc agagagtttt taatcgcctg ttcaaaagat attgctgcac
     3001 agagaatccc gtcaatattc agcagtgaac cggttttgga aactcttcac aacttattga
     3061 tcgagcagct tcgactctat tttattgtcg gcggaatgcc tgaagcagta aaaagattta
     3121 ttgaaacagg gtctgccgcc tcggtgaaag aagttcacga tgacataatt cattcatata
     3181 ttcaatcact tgcaaaatat aattcaaaag tgaacatcga aagcattgaa cagatactgc
     3241 gtgttattcc ttcaaaagtc ggatctcaga ttaaatatac ccgtcttgac cctgacagaa
     3301 gaattgaagt aacaaaaaca tccctcaaca tccttgaaaa agcacttctt gtaaatctta
     3361 ttcactcatc ttccgtctca ggtcttccgc ttggagccga aagtagttca aagatattca
     3421 agcccctgtt tcttgatatc gggctcatgc agtctgtctg cggaattgac cccaaagaaa
     3481 gcataactgc cacagatctt aacaatgtct ataaaggtgc catcgcagaa cagtttgtcg
     3541 ggcaggagct tcttgcggca ggcggatcgg aaaatcacaa gctttactac tggaacagat
     3601 caaagaaaag cagcacggcc gaagtagatt tcgtttttgt cagaaacagt acaatttatc
     3661 cagttgaagt taaaagcgga gcaaaaggaa ggatgaaaag catgcatctc tttcttgaag
     3721 aacaccctga aatcaaaaag ggttttgtcc tttcttcaac tttctcccag caccagcttg
     3781 ttgaaaatat tgtttttgcc ccgatatatt cagagctggg ttgaagtgat cagattttgt
     3841 caagttg
//
LOCUS       DAOOYB010000274         3833 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_28348_length_3833_cov_4.458179, whole genome shotgun sequence.
ACCESSION   DAOOYB010000274 DAOOYB010000000
VERSION     DAOOYB010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3833
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28348_length_3833_cov_4.458179"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="PL056_12045"
     CDS             <1..560
                     /locus_tag="PL056_12045"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HPM47794.1"
                     /translation="VFVDGKKVGTAPGTFKVPMCSEEVVVKTKKGEFKQNLSLQEKKV
                     SKIDTILKPLSGNWSKIAPKEMSWDNAIKYCENLKEDDYSDWRLPTISELRTLIQNCP
                     ATETGGACKVTDSCLSWDDCRNDACDGCKYFEDGRYSKLGDTGWFWSSSLRSDDAALA
                     RFVDFSDGNVNSYHRSAIIYVRCVR"
     gene            611..2623
                     /locus_tag="PL056_12050"
     CDS             611..2623
                     /locus_tag="PL056_12050"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPM47795.1"
                     /translation="MKLLYLWVEEYGILKDAEFNFDSNIRFHYDRENNELKRIESKHE
                     IPDNFFSQKLNNPGGNVVESVSAIIGNNGAGKTSVFRLINRVLWNDVGINFIFIFKTE
                     QEKGEKQDGWFYFKTSGEYYKIKEFNSGEMLNLKDKNDSFVDLKKNNRQDEKPFTIIY
                     HSQFFNPQEYDWVGDSTIDISTTHLMTNDYEWYYNTGTVLKGKTPPHNKLKAHSAMEF
                     IRNINFIKKVIEWEKKGRKFDFGFNYTAGVTLKINEDEEAILTYQMKKGLKEISDCFD
                     NFKKIYKNELPVSETTEKNKVSREIFLFNLSKSIIFNFLRGNASVLKPSSVTINYWRK
                     LQETLDEEKQKNSASWRKVFNNYFTKMSTPFDVTVKKESGEEKVKYSDNKASMILDIM
                     KIFEKIPDNYFTYLGGDIRFDFLTAEGLNSEGFDLFKKIINLYFETNTITHYMLFNWS
                     PRISSGEQSYLNIFSRFISIFETDWTTRCNYKDLIIFFDEIETTLHPEWQRTLVKNLI
                     KFFEEFFIENDHKKHNIEPKHIHLIFATHSPILLSDIPVDNCCFLKREGFETKVVDEM
                     EQTFGANIHSLFADSFFLNGGTIGDFAKNKINGLIQEIKTAKPEYLEENEKSIKNRIE
                     MIGEQIIRGKVISILEERLSTNLISIYSKVRKIEKRLDNIEKKHDD"
     gene            2616..3668
                     /locus_tag="PL056_12055"
     CDS             2616..3668
                     /locus_tag="PL056_12055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47796.1"
                     /translation="MIKITLKESEKIEKIHLEFCKKIFDKNIGNFIKACDTETSWRPF
                     LDFIVEGKNFERLIIGKQVELVKIIEEIKDCKLSFFDKNKKTDICENILKLFHFKSFY
                     YGEKKKKKEKNKNNGDEESWGAYQYVKMLGIKTCPYCNLNYLTMIPRKGKGGGLTPDL
                     DHFFPKSKHPYLALSLYNLIPSCSNCNSRLKRSEDAAELLNPFEGGFGEDFQFRTNLD
                     DVESIYGAAYKIKQLNGEKDKFLIKINDIHELEKNGNESKKSFVKKVNNNKKIFKLEQ
                     QYEFHKDYVREILHKEMIYSKTRIEDLQDNFPDLFTENEVRRSLFSTYFEEKELHKRP
                     LSKLTKDILNEFKIVK"
BASE COUNT         1479 a          516 c          717 g         1121 t
ORIGIN      
        1 atgtttttgt tgatggaaaa aaggtaggaa ctgctcccgg cacatttaaa gtcccgatgt
       61 gcagtgaaga agttgtagtt aaaaccaaaa aaggggagtt caagcagaat ctcagtcttc
      121 aagaaaagaa agtatctaaa attgatacga ttctgaaacc attaagcgga aactggtcta
      181 agattgcacc gaaagaaatg agttgggata atgcaattaa atattgtgaa aatttaaaag
      241 aagatgacta ttcagattgg cggcttccca caatatctga actcagaacg cttattcaaa
      301 actgtcctgc aaccgaaaca ggaggagctt gcaaagttac tgacagttgt ctgtcatggg
      361 atgattgcag aaacgatgct tgtgatggct gtaaatattt tgaggacggg cgatattcca
      421 aactcggtga caccggctgg ttctggtcat cgtccttgcg atcagatgat gcagctctcg
      481 cgaggtttgt ggatttcagc gatggcaatg tgaacagcta ccaccggagt gccattatct
      541 atgttcggtg tgtcaggtag attaatgaaa gaggttgtaa agattgctgt tatttaaata
      601 ggagaattag atgaaacttc tttatttgtg ggttgaagaa tacggaattc tgaaagatgc
      661 cgagttcaat tttgatagta atatcaggtt tcactatgat cgtgaaaaca atgaactgaa
      721 aaggattgag tcaaaacacg aaattccaga caactttttc tcacagaaac ttaataatcc
      781 cggcggaaat gttgttgaat ctgtttcagc aataattggg aataatggag ccggaaaaac
      841 aagtgttttt agattaatta atagagtttt atggaatgat gttggcatca attttatttt
      901 tatttttaaa acagaacaag aaaaaggaga aaaacaagat ggttggtttt atttcaaaac
      961 atcaggtgaa tattataaaa taaaagagtt taactcaggt gagatgctta atttaaaaga
     1021 taaaaacgat agttttgtcg atctcaaaaa aaacaaccga caagatgaaa aaccatttac
     1081 cattatttat cactctcaat ttttcaatcc acaagaatat gattgggttg gggacagcac
     1141 aatagatatt tcgacgacac atcttatgac aaatgactat gagtggtatt acaatacagg
     1201 aacagttttg aaaggaaaaa cacctccgca taataaattg aaagctcatt ctgcgatgga
     1261 atttataaga aatattaatt ttataaaaaa ggtcatagaa tgggagaaaa aaggcagaaa
     1321 gtttgatttt gggttcaatt atactgcggg tgttacttta aaaataaatg aagatgagga
     1381 agcaatttta acttatcaga tgaaaaaagg actgaaagaa atttctgact gctttgataa
     1441 ttttaagaaa atttacaaga atgaacttcc agtgtcagag acgacagaaa aaaataaggt
     1501 aagtagagaa atctttcttt ttaatctttc taaatcaatt atttttaatt ttttaagggg
     1561 aaacgcttcc gttctcaaac catcttctgt tactattaat tattggagaa aacttcaaga
     1621 aacattagat gaagaaaaac aaaagaacag tgcatcttgg cgaaaagtgt tcaacaatta
     1681 tttcacaaaa atgagtacgc cttttgatgt tacagtaaaa aaagagtcgg gtgaagaaaa
     1741 ggtaaaatac agtgataata aagcatcaat gattcttgat ataatgaaga tttttgaaaa
     1801 gattccagat aattatttca cttatttagg aggggatata aggtttgatt ttctaactgc
     1861 agaaggtctt aactctgaag gatttgatct tttcaaaaaa atcataaact tgtattttga
     1921 gacaaacaca ataactcact atatgttatt caactggtct ccgcgaatct catcgggaga
     1981 acaatcttat ttaaatattt tttcaagatt tataagtata tttgaaactg attggacaac
     2041 tcgttgcaat tataaagatt taatcatttt ttttgatgag attgaaacca cacttcatcc
     2101 agaatggcaa agaacattag tgaaaaatct tatcaagttt tttgaagagt tttttattga
     2161 aaacgaccac aagaaacaca acatagagcc aaaacatatc cacctgattt ttgccacaca
     2221 ttccccgatt cttctttcag acattccggt tgacaattgt tgttttttaa aaagagaagg
     2281 atttgagaca aaagtcgttg acgagatgga gcagactttt ggagctaata ttcattctct
     2341 ttttgccgat tcattttttc ttaatggtgg aactatcgga gattttgcaa aaaataaaat
     2401 aaatgggctt atacaggaaa ttaaaactgc taaaccggaa tatttggaag aaaatgaaaa
     2461 aagcataaaa aatagaattg aaatgattgg cgagcagata attcgaggta aagtaatatc
     2521 cattttggaa gaaaggttat ccacaaatct tatcagcata tattcaaaag tcagaaaaat
     2581 tgaaaaaagg ctcgataata ttgagaaaaa acacgatgat taaaataaca ttaaaagaaa
     2641 gtgaaaaaat tgagaaaatt caccttgagt tttgcaagaa aatctttgat aaaaatatcg
     2701 gaaattttat aaaggcgtgt gacaccgaaa cttcttggcg accttttctt gattttatag
     2761 tggaaggcaa gaattttgag agattaataa ttggaaaaca agttgaactt gttaaaataa
     2821 ttgaagaaat taaagattgc aaattatcgt tttttgataa aaacaaaaaa acagatatat
     2881 gtgaaaacat tctgaaattg tttcacttta aatcctttta ttatggagaa aagaaaaaga
     2941 aaaaagaaaa aaacaaaaac aacggtgacg aggaaagttg gggtgcgtat cagtatgtta
     3001 aaatgctcgg aattaagacc tgtccttatt gtaatctcaa ctacttgaca atgatcccaa
     3061 gaaaaggaaa aggaggcgga ttaacaccag atttggacca tttctttcca aaatcgaaac
     3121 atccttatct tgcactttct ctatataacc tcattcccag ttgttctaac tgtaattcca
     3181 gactaaaacg cagtgaagat gctgctgaat tattgaatcc attcgaaggt ggattcggag
     3241 aagacttcca atttagaacc aatctggatg atgttgaaag tatttatggt gcagcctata
     3301 aaataaaaca attgaacgga gaaaaggaca agtttttaat aaaaataaat gatatacatg
     3361 aattagaaaa aaacgggaat gaatcgaaaa agtcgttcgt taaaaaagtt aataataata
     3421 agaaaatctt caagttggag cagcaatacg aattccacaa agattatgtt agagaaatct
     3481 tacacaaaga aatgatatat agcaaaacac gaatagaaga tttgcaagac aactttccgg
     3541 atttattcac cgaaaatgaa gtcaggcgaa gtcttttttc aacatatttt gaagaaaaag
     3601 aactacataa aagaccgttg tctaaattaa caaaagatat tttgaatgaa tttaaaattg
     3661 ttaaatagaa taaaggtggc aaagtggtca aaataaaaga aataaagctg gaaaaactct
     3721 tcgtcaaact tgttaagtga ttgaatatat tgattatgtg taaaaacatg tggaggtgaa
     3781 attgacagaa aaaattaaaa ttcctgataa tttctggcag gaacttgagt ccg
//
LOCUS       DAOOYB010000275         3823 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_28446_length_3823_cov_15.518312, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000275 DAOOYB010000000
VERSION     DAOOYB010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3823
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28446_length_3823_cov_15.518312"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            82..1347
                     /locus_tag="PL056_12060"
     CDS             82..1347
                     /locus_tag="PL056_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004064211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HPM47797.1"
                     /translation="MTNKKKRFSLTNQIFFALIAGALTGVILNIVSAGQHSGFINNWL
                     VSGLFEIVGKMFISAIRMLVVPLVLTSLICGAAGINDIKKLGRVGGKTLVFYLSTTAI
                     AVTIALIVSYIIDPGIGFQTEIQVAEKIETPVLSIAQTIYSIVPVNPFAAFSEGNMLQ
                     VIFFALVFGFSMAALGEKVQNVRTLIEQLNEIVMKMIMVVMATAPFGVFALVANVFAK
                     EGFSAFVPLIKYIICVTVALLIQFSVTYGLALKIFARLSLITFIKKFYSTMLVAFSTA
                     SSNATLPVTMETAEKEMGVSNKIASFTLPLGATVNMDGTAIMQGCAVVFISQVYGIDL
                     SLTALLTVVLTATIASIGTAGVPSVGLITLSMVLTQAGLPVEGIALIMGIDRILDMMR
                     TAVNISGDTVVTLIVAKSEGELDESVFNQ"
     gene            1485..2816
                     /locus_tag="PL056_12065"
     CDS             1485..2816
                     /locus_tag="PL056_12065"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPM47798.1"
                     /translation="MKRFILQKLIEWKERNGRKPLLLRGARQVGKTWILQHFGENYFP
                     GVHYLNFEKDKNLNSLFSNSLDPETILNNYELVYNTSVNENDLIIFDEVQESPNALTS
                     LKYFAELKPSQPVCAAGSNIGIVSSTAGFPVGKVEDIIMYPMNFSEFLQAIQPELAQF
                     IKEKKDFLPIPEILHKKLIDLFLHFYVTGGLPEAVSEFVNNRENTKTAFEKTRMIHER
                     LIFNFVSDFAKHAGAVNAGHIHSVFDHVPQYLSTAVDNSIQRFKFKDVIPGKSRYADL
                     EGPIDWLINSGLLYRINIVEKSETPLISYRKHNIFKLALFDAGLLHTMLNISFSQIIQ
                     QNYGSFKGYIAENFVAGSLVSNRFSPLFSWQRRQSEIEFLIQHEGNIIPVEVKSGSRT
                     KAKSLSVYSEMYSPEIKVIVSLKNIELTNGKTLHIPLYLIDNLRDYLRVTS"
     gene            complement(2819..>3823)
                     /locus_tag="PL056_12070"
     CDS             complement(2819..>3823)
                     /locus_tag="PL056_12070"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM47799.1"
                     /translation="NFAYLNFDVSEDREIISKKEWDRDRELIIFDELHKMKNWKSWIK
                     GVYDKEGVNPALLVTGSARMDTFKKGGESLAGRHFYYRLHPVDVSEASLFMDSKEALE
                     NIMKLGGFPEPFLKGSESFAKRWRRQHTDVIIRQDLLDLEKVREIKGIEIMVELLKNQ
                     VGSTVSYSSLAGDLQVSVPTVKFWLEILEKLFVIFPVRPYHKNIARAILKEPKYYFYD
                     TGSVENGEAAVLENAVACALIKKLHFLEDTQGCKTSLHFIKDKEKREVDFLTVVDGSV
                     LQMIEVKLSDDNFSKNLFHFGSFFDESVKKYQIVHNLKMNKMKEDVRMIQAHEFLKDL
                     "
BASE COUNT         1116 a          789 c          740 g         1178 t
ORIGIN      
        1 tgcagagttg aaacaaaagg gggaaattat cttgtctgta aagacggacc ggtgtttaac
       61 ggatacgata ttttttaatc tatgacaaat aagaaaaaaa gattttcact cacaaatcag
      121 atattttttg ctcttatcgc cggagcactt acaggggtca ttctcaacat tgtttcggca
      181 ggtcaacata gcggatttat taataattgg cttgtttcag gtctttttga aattgtcgga
      241 aaaatgttca tttcagcaat tcggatgctt gttgttccac ttgttctgac gagcttaatc
      301 tgcggagcgg cagggataaa tgacataaaa aaacttggcc gggtcggtgg aaaaaccctc
      361 gtattttatc tttccacaac tgcaattgcc gttaccattg ctttgatcgt ttcatatatc
      421 atcgaccccg gaatcggctt tcaaacagag attcaggtgg cagaaaagat tgaaacaccg
      481 gttctttcaa ttgcccagac gatatattca attgttcctg tcaacccttt tgccgctttt
      541 tcagaaggga acatgcttca ggtgatattt ttcgctcttg ttttcggttt ttcgatggcg
      601 gctctgggtg aaaaagttca gaatgtgaga actctgatcg agcagttaaa tgagatagtt
      661 atgaaaatga tcatggtcgt gatggcaact gcccctttcg gagttttcgc ccttgtcgcc
      721 aatgtttttg caaaggaggg cttttcagca tttgttccgc tgattaaata tataatctgt
      781 gtgactgtgg cacttttgat ccagttttca gttacttacg gacttgcatt gaaaatattc
      841 gcccgtcttt cactaattac ttttataaaa aaattctatt ccacaatgct tgtcgctttt
      901 tcaactgctt caagcaacgc aacccttcct gtcacaatgg aaactgctga aaaggaaatg
      961 ggagtttcaa ataagatcgc atcattcact ctgccgcttg gagcaactgt caatatggat
     1021 ggaactgcga taatgcaggg ttgtgccgtt gttttcattt ctcaggttta cggcattgat
     1081 ctttctttga cggcactttt aactgttgtc cttactgcaa cgatcgcaag catcggaaca
     1141 gcgggcgttc caagtgtcgg gctgataacg ctctcgatgg tcctcactca ggcgggactg
     1201 ccggttgaag gaatcgccct cataatggga atcgacagaa tactcgacat gatgagaacc
     1261 gccgtaaaca tctcaggtga caccgtcgta acgctcatcg tcgccaaaag cgaaggggaa
     1321 ctcgacgaat cggtctttaa tcagtaaaac agctgaatcc cgcaaaaagt gtcggatttt
     1381 aacactttta tcatgtaaaa agtgctggat tttacatttt tatcttgcaa aaagtgtagt
     1441 tttgacacat tatttacatg caaaaagtgt aaaagaggtt aaaaatgaaa agatttattt
     1501 tacagaaact gatcgaatgg aaagagcgga acggaagaaa accgctgttg ctaagaggcg
     1561 ccagacaggt tggaaaaaca tggatattgc aacatttcgg tgaaaattat tttcccggag
     1621 ttcattacct gaactttgaa aaagataaaa acctcaactc acttttttct aattcgcttg
     1681 atcctgaaac gattttaaat aattatgagc tggtttacaa cacatctgta aacgagaatg
     1741 atctgataat ttttgatgaa gttcaggaat caccgaatgc cctcacttct ttaaaatatt
     1801 ttgctgaact taaacccagc cagcccgttt gcgccgccgg atcaaatata gggattgtct
     1861 cttcaacggc aggatttcct gttggaaaag ttgaagatat cattatgtat cccatgaatt
     1921 tttctgagtt tctgcaagcc atacaacctg agcttgctca attcataaaa gaaaaaaagg
     1981 attttttgcc aattcctgaa attcttcaca aaaaacttat tgatcttttt cttcattttt
     2041 atgtaacagg aggacttcct gaagctgtat cagagtttgt caacaacagg gaaaacacaa
     2101 aaaccgcatt tgaaaagacc cgtatgatcc atgaacggct catttttaat ttcgtgtctg
     2161 attttgctaa acatgcaggg gcagtaaatg caggtcacat ccattctgtt tttgatcatg
     2221 ttccgcagta tttaagcacc gctgttgata attccattca gcggttcaag ttcaaggatg
     2281 tcattccggg aaaatcgaga tacgcagatc ttgaaggccc gattgactgg cttataaatt
     2341 caggtcttct gtaccggatc aatattgtcg aaaaatcgga gacacctctt ataagctacc
     2401 gcaaacacaa catatttaag ctcgcattgt ttgacgcagg acttcttcac acaatgctga
     2461 atatttcgtt ttcacagatc attcagcaga attatggctc ttttaaagga tatatcgctg
     2521 agaattttgt cgccggctca cttgtttcaa accgtttctc accacttttc tcatggcagc
     2581 gccgccagtc tgaaattgaa tttctaattc agcatgaagg gaatatcatt ccggttgaag
     2641 ttaagtccgg ctcaagaaca aaagcaaaaa gcttgtcagt ttattcagaa atgtattcac
     2701 cggaaattaa agtgatcgta agtttaaaaa acatcgaact tactaacgga aaaacccttc
     2761 acattccact ttatctcatt gataacctca gggattatct aagggtaact agctgacatc
     2821 acaaatcttt aagaaattcg tgtgcctgaa tcattctgac atcttctttc attttgttca
     2881 ttttgagatt atggacgatc tgatattttt ttactgattc atcaaagaac gagccgaagt
     2941 gaaatagatt ttttgaaaaa ttgtcgtcac tgagcttgac ttctatcatc tgcaaaactg
     3001 atccgtcaac cactgtgaga aagtcaactt cccgtttttc cttgtctttt atgaaatgaa
     3061 gagaggtttt acacccctgt gtgtcttcaa gaaaatgaag ttttttaatg agagcacaag
     3121 caacagcgtt ttcaagaacg gccgcttcac cgttttcgac tgaaccggtg tcataaaaat
     3181 aatacttcgg ctctttgaga atggctctgg caatattttt atgataaggt cgcacaggaa
     3241 aaatgacaaa cagtttttca aggatctcaa gccagaattt cactgtcgga acagaaacct
     3301 ggagatcgcc tgcaagagaa ctgtatgaaa cagttgaacc cacctgattt ttcaaaagtt
     3361 cgaccattat ctcaatccct tttatttcac gcactttttc aagatcgagc agatcctgtc
     3421 tgattatcac atctgtgtgc tgacggcgcc acctttttgc aaaactttca ctccctttta
     3481 aaaatggttc aggaaatccg ccgagtttca ttatgttctc aagcgcctct tttgaatcca
     3541 tgaataacga tgcctctgaa acatcaaccg gatgaaggcg gtaataaaaa tgtcttccgg
     3601 caagtgactc cccccctttt ttgaaagtgt ccatccttgc tgaaccggta acaagaagag
     3661 ctggattcac cccttccttg tcataaaccc cctttatcca ggatttccag ttcttcattt
     3721 tgtgaagctc atcaaaaata ataagctcgc gatctctgtc ccactctttt ttcgagataa
     3781 tttctctgtc ttctgaaaca tcaaagttta aataggcgaa gtt
//
LOCUS       DAOOYB010000276         3821 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_28460_length_3821_cov_7.458046, whole genome shotgun sequence.
ACCESSION   DAOOYB010000276 DAOOYB010000000
VERSION     DAOOYB010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3821
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28460_length_3821_cov_7.458046"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="PL056_12075"
     CDS             <1..528
                     /locus_tag="PL056_12075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47800.1"
                     /translation="YGTDASWLCDLSKNPKKITDCKRIGKENEVAGFPKFDRDNPYRI
                     AYRPMADGIPRNRFVIMDISQDPWKIEKEFDIPPTEEKYLKLQLTDFKSDIMLYREDI
                     SIDGGYDGKLCYYRIDKGKVYCSKSIEGQTSYGHGWPAFEGKYLFWQPAYKAGYILRD
                     MECYCKEESVCPFEE"
     gene            736..1692
                     /locus_tag="PL056_12080"
     CDS             736..1692
                     /locus_tag="PL056_12080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47801.1"
                     /translation="MNQIFKILTVFVFSFVIFSCGTEDEESESVTDNETSDKSEISDD
                     LETSDDSTENEIETDNEEVADNENSNAVCGNSTIEDGESCDGGTVSCEALGSQYGYGI
                     APCKSDCTGFDTSDCPLKSDGAKPYGKMTINFKTDFIFDYDTADSQGDSYFDAHPEGI
                     MTTPAFTGLFGYGKNAQIPFEGSSGDTADVVAYHWDDGEIYVVQHMFSGQSVSAPRIL
                     LIVNDSMKKGDILAMCPDKSFLQVIQLTNSGSIDCLVGFALDETLPQLSVDEATDITA
                     KDGGKLYLSGSDIIIYHPTDTPDGDLSQQFISQGAKICPVVE"
     gene            1737..3761
                     /locus_tag="PL056_12085"
     CDS             1737..3761
                     /locus_tag="PL056_12085"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPM47802.1"
                     /translation="MFKSLCLLAFIFISFNVCGYDTFYFDGKGNPQYFKVVEPATANS
                     WPVVTFYGGKPSRLNGTIGITFRNEIPLEKRKELFKKHSLNVIYHDLSDPDYYLLNMK
                     KGVDPFQLSVNIYKTGFVKWAQPDMEAHLEIAGIDDDAMDDEDIIETMPDPVIPDDTW
                     YTQYPVGKWHHEKIRSNYAWALEKGSPDIKIAIIDNGVDLEHEDLKENIVAGSGWNFV
                     DENDNASFKPHTATTAYQSRIVLAHGTSVAGIAAAKGNNSKGVAGICWDCGIIPVRFL
                     DMDIGTGYVDTFHKTYLALKHAVDQGASVINNSWVWWNQQSCAPVPFSSFVEQAVQYA
                     AKNGRDGKGTIMVWAAGNNFCDTENVKNFDNDHIVVVSALRHNTLDNGKGYKSIYSNY
                     GRRIDVSAPAGDPDDNPPYKGIVTCDISNGLGGDAGHYATNPAFSGTSAAAPVVSGAI
                     ALMLSAKPEMNIFEALHCLKKGAAAGVEKRILDGDMVPDHNIINKDRQVIGQCEWNDI
                     KDPYFNVVMKHNTCFGYGYLDVYEMVKMAINDECTYELPLCTKDEDCPEDQLCNTDTG
                     LCVLEIGCKSDEDCLTYQKCDIESGLCYFPENPDNGNETPDNNTEDPDNNTEIPDSNT
                     TPDNGNKDDTSELPDETNEETDNISEQPDNNGIDEFIEDESVGCGCTLIY"
BASE COUNT         1199 a          755 c          847 g         1020 t
ORIGIN      
        1 tatggaacag atgcatcctg gctctgtgat ctttcaaaaa atccgaagaa aatcacggat
       61 tgtaaaagaa taggtaagga aaatgaagtt gccggatttc caaaatttga tagggacaat
      121 ccgtaccgta ttgcctatag gcctatggcg gacggaatac cgagaaaccg ttttgttata
      181 atggatatat cacaagatcc atggaagata gaaaaagagt ttgatattcc gccaactgaa
      241 gaaaaatatc tgaagctcca gctgactgat ttcaagtcag acatcatgct ttatagagag
      301 gacatttcaa tagacggcgg ctatgacggc aagctttgtt attacagaat tgacaaaggg
      361 aaagtctatt gttccaaatc gatagagggg cagacaagtt atggacatgg ctggccggca
      421 tttgaaggaa aatatctgtt ctggcaacct gcatataaag cagggtatat tctcagagat
      481 atggagtgct attgcaaaga agagagtgtc tgcccgtttg aggaatgatc ctgtcttgtc
      541 ctgaaatatc ctgaacagtt ttaatcaaaa caatatattt ttttgcctta acagttcagg
      601 agcagtccga atatgcaaaa tcggacttga aaaagtgact gttgtcgccc tgatttgcaa
      661 aaaaaggcga attcatccag ctaagatcat attttaactt gtttggcgat cattatattt
      721 tttcaggagg cattaatgaa tcagatcttt aaaatcctta cagtttttgt attcagtttt
      781 gttatttttt catgcggcac tgaagatgag gaatcagaat ctgttacaga caacgaaaca
      841 tcagacaaat cggaaatttc tgatgattta gaaacttcag atgattcaac agaaaacgaa
      901 attgagacag ataatgaaga agtcgccgac aatgaaaaca gcaatgcagt ctgcggcaat
      961 tcaacaattg aagatggtga atcttgcgac ggcggaactg tatcatgtga agcactcggc
     1021 tctcaatacg gttacgggat tgctccatgc aaatctgact gtaccggttt tgatacatct
     1081 gactgccctt taaaatcaga cggtgcaaaa ccttacggaa agatgacaat taattttaag
     1141 accgacttta tttttgacta tgacactgcc gattctcaag gtgacagcta ttttgatgct
     1201 cacccggaag ggataatgac aactcccgca ttcactggac ttttcggata cgggaaaaat
     1261 gctcaaatac cttttgaagg atcatctggc gatacagcag atgttgttgc atatcactgg
     1321 gatgacggtg aaatttatgt ggttcagcac atgttctcag gtcaatctgt ttctgcaccg
     1381 agaattttgc ttatcgtaaa tgattcaatg aaaaaaggcg atattcttgc aatgtgcccc
     1441 gacaaatctt ttttgcaggt gattcagctt acaaattcag gttcaataga ctgccttgtc
     1501 ggctttgcac ttgatgaaac tctgccgcag ctttcagttg atgaagcaac agatattacc
     1561 gcaaaagacg gcggaaaact ttacctttca ggcagtgaca taataattta tcatcccacc
     1621 gatacaccgg atggagacct ttctcagcag ttcatcagtc agggggcaaa gatttgtcct
     1681 gtagttgaat aaactgtcag gatatacaaa atcggactaa aaatgtgagg tgtttcatgt
     1741 tcaagtcatt gtgtttatta gcttttatct tcatctcctt taatgtttgc ggttatgaca
     1801 cattctattt tgacggcaag ggaaatcccc agtatttcaa ggtggttgag ccggctaccg
     1861 caaattcatg gcccgttgtc actttttatg gcggcaaacc ttcacggctt aatggaacga
     1921 tagggatcac attcagaaat gaaattcccc tggaaaaaag gaaagaactt ttcaaaaagc
     1981 actcgctcaa tgtgatttac cacgacttga gcgatcctga ctattatctg ctgaacatga
     2041 aaaaaggggt tgatccgttt caactgtctg taaacattta taaaaccggc tttgtcaagt
     2101 gggctcagcc cgacatggaa gcacaccttg agattgcagg aatagatgat gatgcaatgg
     2161 atgacgaaga tattattgag acaatgcccg atccggtcat tccggatgac acttggtaca
     2221 ctcagtatcc ggttggaaaa tggcatcatg aaaagatccg gtcaaattat gcatgggcac
     2281 ttgaaaaagg gagtcccgac ataaagatcg caataattga taacggtgtc gaccttgaac
     2341 atgaagatct caaagaaaac atcgttgccg gctccggctg gaatttcgtt gatgaaaatg
     2401 acaatgcatc ttttaaaccg cataccgcta caactgcata tcagtcaagg atcgtccttg
     2461 cacacggaac aagtgttgca ggcattgccg ccgcaaaagg gaacaattca aaaggtgttg
     2521 caggtatctg ctgggattgc ggaataatcc ctgtgaggtt tcttgatatg gatatcggta
     2581 cgggctatgt tgacacattc cacaaaacat atctggcact taaacatgct gttgatcagg
     2641 gcgcttcggt cataaacaac tcatgggtct ggtggaatca gcagagttgc gctccggttc
     2701 ctttcagttc ttttgtagaa caggcggttc agtatgcggc aaagaacggt cgtgacggta
     2761 aagggacaat aatggtgtgg gctgccggca acaatttctg tgatactgaa aatgtaaaaa
     2821 attttgataa cgaccacatt gttgtagttt cagctttaag gcacaacact cttgataacg
     2881 gcaaaggata taaatctatc tacagcaact acggcaggag aattgatgtt tctgctccgg
     2941 caggagatcc cgatgataac ccgccgtaca aaggaattgt aacctgcgat atttcaaacg
     3001 ggcttggcgg agatgccggt cattatgcga caaatccagc attttcaggc acttccgcag
     3061 cggctcctgt cgtttcaggc gcaattgccc tgatgctttc tgctaaacct gaaatgaaca
     3121 tattcgaggc acttcactgc ctgaaaaaag gggcggcggc aggtgttgaa aaaaggatcc
     3181 ttgacggcga tatggttcct gaccacaaca ttataaataa agatcgtcag gtgataggtc
     3241 aatgtgaatg gaatgacata aaagacccgt attttaatgt agttatgaaa cacaacacct
     3301 gttttggcta tggctatctc gatgtttatg aaatggtaaa aatggctata aatgacgaat
     3361 gcacttatga acttccatta tgtacaaaag atgaggattg tcccgaagat cagctctgca
     3421 acaccgacac aggactttgc gttcttgaga tcggttgtaa atcagatgag gactgcctga
     3481 catatcagaa atgtgatatt gaaagcggtc tgtgttattt ccccgaaaat cccgacaacg
     3541 gaaacgaaac acctgacaat aacacagaag atcccgataa taatacggaa atccccgaca
     3601 gcaacacaac tcccgacaac ggaaataaag atgacacatc agaacttcct gatgaaacca
     3661 atgaagaaac ggacaatatt tcagaacagc ccgacaataa cggaatagat gaattcattg
     3721 aagatgaatc tgtcggctgc ggatgtactc tgatttatta agaaactagt agcagtatcc
     3781 ctgaatacaa ggcattcctg aacaacactc actaacacct g
//
LOCUS       DAOOYB010000277         3814 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_28542_length_3814_cov_20.368449, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000277 DAOOYB010000000
VERSION     DAOOYB010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28542_length_3814_cov_20.368449"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            39..1613
                     /locus_tag="PL056_12090"
     CDS             39..1613
                     /locus_tag="PL056_12090"
                     /inference="COORDINATES: protein motif:HMM:NF016098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HPM47803.1"
                     /translation="MLQTDKIKNMAYLYNPANYSEEELIENFIVRLNEFNSIFQVIKN
                     DDMKHPPQHFLIQGLRGNGKTTLLRRLFYEVKNDPQLSKNLIPVIFDEEEYGVRTLYK
                     LWERIAENIEDNYSEFTGIYDEMMKHVENDDYEETAYKILKNKLNDKGMKLVLFFDNF
                     GEVLEKFNEKEARRLREILMGENFLRIVGASSIMLESFYDYSKPFYEFFKIIQLNGLT
                     KTEALEFLKKIAENHDKPEVVKIIENHPERVETLRVLTEGVPRTMLLLFEIFADNDNG
                     NSFEDLDLVLDRVTPLYKHRMDNMSEIQQEIVDIIARNWDGIEVSTIAEKSKMDSKSI
                     SSQLNVLSKNNIISKISTNTKNNLYILKERFFNIWYLMRYGRKNDKNRVLWLTRFLEA
                     WLSNKKIDTVMIFENNLGYGAVSKIINDLRSDKIEEGVLAAREFLSHEKVYEKYTEDI
                     TLLLIFMMAKNQYNSVLKIFNENNFEIKDRFKPVYYALMHFMKDKFPNEYLKMGSELK
                     ETVEEIIKEVEKYRNW"
     gene            complement(1623..2975)
                     /locus_tag="PL056_12095"
     CDS             complement(1623..2975)
                     /locus_tag="PL056_12095"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HPM47804.1"
                     /translation="MSSTVKIKSVQTFKLEKVPDLIHLSYLTGLKKGRVLFFSSGLHP
                     VHGRFTVLSLNPLFSIKSDFGELLKALFSIKDFSQINGMPMALFSGWMNYECLEFIEK
                     VDANKKSLQVVPDFHFIFSDSFIVIDNFEKRAELVLLETNHSVTSINERKQLYFEELA
                     ADIPDIDYELGAIKAKALISKKKYVESVNEIRRRIGAGDVYQVNFTYPVEIKTEIPSW
                     VLFYKYLAKNHADYAAFINSSEVEVLSISPECFFRIENGEKVSSFPIKGTIKKGINQQ
                     EDQALKFELAQSRKDFAELAMIVDLIRNDIGKVANPGTVKVEKHAELFEFPTLFHLVS
                     TVSGEIEKEKAIELFKSLFPGGSITGAPKISAMKIISELEEYKRNIYTGAIGFTGLNG
                     TAVFNIPIRTVQRVDNVLIYSTGGGITFKSDPEKEFEETLVKTRAFLNIFDEAEMVFE
                     "
     gene            3112..3810
                     /locus_tag="PL056_12100"
     CDS             3112..3810
                     /locus_tag="PL056_12100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47805.1"
                     /translation="MLKSINFMVFVSVFILFSFVSCSNDDPLATEDLDKDNIATDNEE
                     NDSQTGDDSVIIDENETIDQKTVPDENEETDVDLSDEVLTELDDESVDEEVNDDESSN
                     LNTGDTSGTQAATGKSGAPCTKDEDCTNWTGTDEQYAICLLPTEGYPGGYCSFMCDSS
                     VTLNYGCNGFGGVYYGYGTLGDGYCFHPCDDPTDCRVGYRCSQTVGACMPDCAVDACV
                     LGTCDETEKVCLDK"
BASE COUNT         1287 a          667 c          720 g         1140 t
ORIGIN      
        1 attcaattca cctttattaa aaatgtggtg gtgcagaaat gttgcaaact gataaaatta
       61 aaaatatggc gtatctttac aatccggcca attactctga agaggagctt attgagaatt
      121 ttattgtccg gctcaatgaa ttcaattcga tctttcaagt gataaaaaat gatgatatga
      181 agcatcctcc acagcatttt ttaattcagg gattgagagg aaatggaaaa acaacacttt
      241 taagacggct tttctacgaa gtgaaaaacg atcctcaact tagtaaaaac ctgatacctg
      301 taatttttga cgaagaggaa tatggagtca gaacacttta taaactgtgg gaaagaattg
      361 cggaaaacat cgaggataat tattctgagt ttacaggcat ttacgatgaa atgatgaaac
      421 atgttgaaaa tgatgattat gaagaaacgg cttataaaat cttaaaaaac aaacttaacg
      481 acaaaggcat gaaactggtt ctcttttttg ataattttgg ggaagttctt gaaaaattta
      541 atgaaaaaga agctcgcaga ctccgcgaaa ttctgatggg ggaaaatttt ttaagaatcg
      601 tgggcgcttc ttcaataatg ctagaatctt tttatgatta ttccaaaccg ttttatgaat
      661 tttttaaaat aattcagcta aatggtctga caaaaacaga agctcttgaa tttttaaaga
      721 aaattgctga aaaccatgat aaaccggaag ttgtcaaaat aatcgaaaat catcctgaaa
      781 gggttgaaac attaagggtt ctgacagaag gcgttccccg gacaatgctc cttctttttg
      841 aaatatttgc cgacaacgac aatggaaatt cctttgaaga tcttgacctt gtgcttgatc
      901 gtgtcacgcc actttataaa cacagaatgg ataatatgtc agaaattcag caggaaatag
      961 ttgatatcat tgccagaaat tgggacggca tcgaggtttc aacaatcgct gaaaaatcaa
     1021 aaatggacag caagtcaatc tcttcacaac tcaatgtcct ttctaagaac aatatcatct
     1081 caaaaatatc cacaaacaca aaaaacaacc tttacattct caaagaaagg tttttcaaca
     1141 tctggtatct tatgcgctac ggcagaaaaa atgataaaaa ccgggttctg tggctcacca
     1201 gatttcttga agcgtggtta tcaaacaaaa aaattgatac cgttatgatt tttgaaaata
     1261 atttgggata tggagcagtt tcaaaaatta ttaatgattt aagatccgac aaaatcgaag
     1321 aaggagtcct tgctgcaaga gaatttcttt cacatgaaaa agtttatgaa aaatacactg
     1381 aagacattac gcttcttttg atttttatga tggcaaaaaa tcaatataat tcagtgctta
     1441 agatatttaa tgaaaacaat tttgagatca aggacagatt caagccggtg tattatgctc
     1501 tgatgcactt catgaaagat aaattcccca atgaatatct caagatggga agtgaactga
     1561 aagaaacggt tgaagagatt attaaagaag ttgaaaaata caggaactgg taaaaaaccg
     1621 aattactcaa aaaccatctc cgcttcatca aatatattaa ggaaagccct tgtttttact
     1681 agagtttctt caaattcttt ttcggggtcg cttttgaaag ttatcccgcc gcctgttgaa
     1741 tagatgagca cattgtcgac tctttgaact gtccggatcg ggatgttgaa aacggcggtt
     1801 ccgtttaatc ctgtgaatcc gatagctccg gtatagatat tccgtttata ttcctcaagt
     1861 tctgatatga tcttcattgc acttatcttc ggtgctcctg tgatcgaacc gcctggaaaa
     1921 agggacttga aaagctctat cgctttttct ttttcgattt cgcctgaaac agttgaaaca
     1981 aggtggaaaa gtgtaggaaa ttcaaaaagt tcagcatgtt tttcaacttt gacagttcca
     2041 gggtttgcaa cttttccgat gtcatttctg attagatcaa cgatcatggc taattctgca
     2101 aaatcttttc tgctctgagc aagttcaaac ttaagtgcct gatcttcctg ctgatttatc
     2161 ccctttttaa ttgtcccttt tatcgggaaa ctgctcactt tttcaccatt ttcaattctg
     2221 aaaaaacatt caggagaaat tgaaaggact tcaacttctg aactatttat gaatgccgca
     2281 taatcggcat gattttttgc cagatatttg taaaaaagaa cccacgacgg aatttctgtt
     2341 ttaatttcaa ccggatatgt aaaatttacc tgataaacat cacctgcccc gattcttctt
     2401 cttatctcat tgactgattc aacatatttt ttctttgata tcagagcttt tgcttttata
     2461 gcaccaagtt cataatcaat atcaggaata tctgctgcaa gttcttcaaa ataaagctgt
     2521 ttcctttcat tgatcgaagt aaccgaatga ttcgtttcaa gaagaacaag ttcagccctt
     2581 ttttcaaagt tgtcaatcac aataaagctg tctgaaaaaa taaaatgaaa gtcgggaaca
     2641 acttgcagac tctttttgtt tgcatcaact ttctcaatga attcaagaca ttcgtaattc
     2701 atccagcccg aaaaaagagc catcggcatt ccattgatct ggctgaaatc tttaattgaa
     2761 aaaagagctt tgagcaattc cccaaaatct gacttaattg aaaaaagagg attaagcgat
     2821 aaaacagtga acctgccatg aaccggatga agccccgaac tgaaaaaaag aacgcgaccc
     2881 tttttcaacc ctgtcaaata tgaaaggtga attaaatcag gaactttttc aagcttaaaa
     2941 gtttgaactg attttatttt aactgtactg ctcaaaaaaa ctcctgaaaa gaacaaggca
     3001 agtttaacaa aataaaagag ttttgcaaaa tcagattctg gaccattata tttgacttaa
     3061 aaaattatcc tcatatcctt atttccgtgt gtgtttagtg agggaaaaaa tatgttaaaa
     3121 agtattaatt ttatggtttt tgtatcggtt tttattcttt tttcatttgt ttcgtgctca
     3181 aacgatgatc ctttggcaac ggaagatctg gataaggaca atatcgcgac agacaatgaa
     3241 gaaaatgatt ctcaaacagg tgatgactca gtaattattg atgaaaatga aaccattgat
     3301 cagaaaactg ttccggatga aaacgaagaa actgatgttg acttgtccga cgaagtttta
     3361 acggagctgg atgatgaatc agtagatgaa gaagtaaatg atgatgaatc ttcaaatctc
     3421 aacacgggtg atacaagcgg aacacaggcg gcaacgggaa aatccggagc accgtgcaca
     3481 aaagatgaag actgcacaaa ctggacaggg acagatgagc agtatgcgat ctgtcttctt
     3541 ccaacagaag ggtatccggg cggttactgc agtttcatgt gtgacagctc agttacatta
     3601 aactacggat gcaacggttt cggcggagtt tattacggtt acggaacgct gggagacgga
     3661 tactgctttc atccatgtga cgatccaacc gattgcagag tcggatacag atgttctcaa
     3721 actgtcggtg cctgcatgcc tgattgtgct gtagatgcat gcgtgcttgg aacatgtgac
     3781 gaaaccgaaa aagtgtgtct ggataaatag ttct
//
LOCUS       DAOOYB010000278         3804 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_28638_length_3804_cov_9.645772, whole genome shotgun sequence.
ACCESSION   DAOOYB010000278 DAOOYB010000000
VERSION     DAOOYB010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3804
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28638_length_3804_cov_9.645772"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1796)
                     /gene="pepF"
                     /locus_tag="PL056_12105"
     CDS             complement(<1..1796)
                     /gene="pepF"
                     /locus_tag="PL056_12105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HPM47806.1"
                     /translation="MKTRNEMDSKFMWKPEHIYGSSEEVTDEFQLIEKATAQMPKFAG
                     RLSEKESLMKALRTSSDAMRRVMKLESYAHRNRDVDMAEGYWQELSGMVESAQTSLAA
                     ATSYMEPELLSYEEDVLKGFRDDPDFSVFTRYFDALLRNKPHTLSDKEEMIMAQFGGL
                     SLAEYEIYSTFSSTDMKFPKIEINGELVEVNMPTYARYRASDDREIRKKVFDAFWKTY
                     DGYKNGISKMLHYQIKYYSTVAKIRKFDDALESEMNSNELPNGFFYTLRDNIRAILPA
                     LHEYLAVKKEVLKIPDQGYHDVYAKMVTGGSNRKFEFNEAVKLINDAMKPMTDEYRNV
                     LSESMKPENGWVDIYPNEGKRSGAYMSGEAYDTHPFVLLNHIDDYNSASTLAHEMGHA
                     MHSYYSNGNQPFEKSQYTIFVAEVASTFNEIMLINHLLDTTTDKEERKFLLNHFIEMI
                     RSTVFRQMQFAEFEDEIYKKVESGEALTPDFLNHVYGDTLSRYYGEEYGLMKIDELYK
                     NEWAFVPHFYYNYYVYKYVVGFIGALSLAESVRTGLVSPQQYIDGFLKAGSSKPPVEI
                     LKDAGVDMTSIEPYKLIEKVFVEKLKELKELL"
     gene            complement(1809..2621)
                     /locus_tag="PL056_12110"
     CDS             complement(1809..2621)
                     /locus_tag="PL056_12110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47807.1"
                     /translation="MKRFCRILISLVFILSSLMLFSQNRPVTLKEKILMERIEKEKGE
                     VSLAMKVTESELEVIKTMSFNQLLKYRKLDFLKRSSGIMKSNCLFFDSECKKVYNMSI
                     VYLKNEMKLVKKELDGFVKLMEESHKRLSELKEKYALLEKGVEVEISEIRIKPAIPEL
                     RNGYKCVNTPGWNPMRGIFVSPSEINDLPFQSFIKDVVKLDSGGYVIMVEAGEFTMNF
                     SYVKTPLVKAGEIVKMGRKLFTGSAENPVMPGNILIFITKNGKFANPGFMCK"
     gene            complement(2605..3792)
                     /locus_tag="PL056_12115"
     CDS             complement(2605..3792)
                     /locus_tag="PL056_12115"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HPM47808.1"
                     /translation="MKLLMVIVMFFILSSCSTHEGVVGELPKNAIKTGMKLNDGKVEF
                     MGFYTEPIKPRHGESFKIVTFWKFDEQLGDGWKLFYHFEDEKGEEHFKYDHEFIDGKV
                     KKLALGKIIKDEAVIKELPRHFDTDTMFVRGGFFKGKERTKPEKQFNDGKNRLNMAQI
                     KITKPNILRKKMEVYTIAGNSRKQTKVDGEFKESYWANASKDDKFWISSGEGLSSVKT
                     SVMAVMDHNYLYIGFDAEDSDIYAEQKNNDDPLYDTDDVVEIFIDPRGEGKIYYEMQV
                     SAAGVKFDSKFNGRRKNRDDSWDSKIKYAVKVDGKLNDSNEKDKGWRAEIAIPWSSIE
                     DAPVNPPKDGDTWKVFLYRINRHTGKKSKSDDFTAWTPAYAKDFHNIKFMGELVFVYE
                     EIL"
BASE COUNT         1002 a          881 c          632 g         1289 t
ORIGIN      
        1 tacagaagtt ctttcagttc cttgagtttt tcgacaaata ctttttcaat gagtttgtat
       61 ggctcgatac ttgtcatgtc aacccctgca tccttgagaa tttcaacggg cggtttcgag
      121 cttccggctt ttaaaaatcc gtcaatatac tgctggggag aaacaagacc ggttctcaca
      181 ctttcggcga gtgacagtgc accaataaac ccgacaacat acttgtaaac ataatagttg
      241 taatagaaat ggggaacgaa cgcccactcg ttcttataaa gttcgtcaat tttcatcagt
      301 ccgtattctt caccgtaata tctgcttaaa gtgtcgccat agacatggtt aagaaaatcc
      361 ggcgtaagtg cttctcctga ctcaactttt ttatatattt catcttcaaa ttcagcaaac
      421 tgcatctgcc tgaaaacagt tgagcggatc atttcaatga agtgatttaa gagaaatttt
      481 ctctcttctt tatcggttgt tgtgtcaaga aggtggttta tcagcattat ttcgttgaat
      541 gtgctcgcaa cttcggcaac aaaaatggtg tattgcgact tttcaaacgg ctgatttcca
      601 tttgaataat atgagtgcat cgcatgaccc atttcgtgag caagggttga cgctgaattg
      661 tagtcatcaa tgtgattaag caacacaaaa ggatgcgtgt cataagcttc tcctgacatg
      721 taagcaccac tccgtttccc ctcatttgga taaatatcaa cccagccgtt ttccggcttc
      781 atactttctg aaaggacatt tctatattca tccgtcatcg gcttcattgc gtcatttatc
      841 agcttaactg cctcattaaa ctcgaatttt ctattactcc cgccagtcac catctttgca
      901 tagacatcgt ggtatccctg atcgggaatt ttgagaactt cttttttaac ggcaagatat
      961 tcgtgaagag caggaagtat cgctctgatg ttatccctta aagtatagaa aaaaccgtta
     1021 ggaagctcgt tggaattcat ctcagattcc agagcatcat caaattttct tatttttgcc
     1081 accgttgaat agtactttat ctgataatga agcatctttg aaatgccgtt cttgtaaccg
     1141 tcataagttt tccagaacgc atcaaacacc ttttttctta tctctctgtc atcacttgcc
     1201 ctgtatctcg cataagtcgg catattcact tcaacaagct ctccgtttat ctcaattttc
     1261 gggaatttca tatctgttga cgaaaaagtg ctgtaaattt catattcagc aagcgataat
     1321 ccgccaaact gtgccatgat catctcttcc ttatcggaca gagtgtgcgg cttatttcta
     1381 agcaatgcat caaaatatct tgtgaaaacc gaaaaatcgg gatcgtctct gaaccctttg
     1441 agcacatcct cttcatacga aagaagctcc ggttccatgt atgaagttgc agcggcaagt
     1501 gaagtctgtg cagattcaac cattcctgaa agttcctgcc agtacccttc cgccatatct
     1561 acatcacggt tcctgtgagc ataactctcc agtttcatta ctcttctcat tgcatcactt
     1621 gaagttctga gtgctttcat caacgactct ttttcactca atcttcctgc aaatttcggc
     1681 atttgcgccg tcgccttctc aattaactga aactcatcgg taacctcttc ggatgaaccg
     1741 taaatatgtt ccggtttcca catgaatttt gaatccattt catttcttgt tttcattgtt
     1801 taattctcct atttacacat aaatccgggg tttgcgaatt tcccgttttt tgttatgaag
     1861 atcagtatgt taccgggcat gacggggttt tccgccgacc ctgtgaaaag ttttctgccc
     1921 attttgacta tttcgccggc ttttactagt ggtgttttga catagctgaa attcatcgtg
     1981 aattctcctg cttcgaccat tatgacatag ccgcctgaat caagcttgac cacatctttt
     2041 ataaaactct ggaaagggag atcgtttatt tcagaagggg aaacaaaaat acctctcatt
     2101 ggattccaac ccggcgtgtt gacgcattta tatccgttcc tgagttcggg gatagcgggt
     2161 ttaattctaa tttcacttat ttcgacttca accccttttt caagaagggc atacttttcc
     2221 tttaactcac tcagtctctt gtgactttcc tccatgagct taacaaaccc atcaagctct
     2281 ttttttacca gtttcatttc atttttaaga tatacaattg acatattgta gaccttctta
     2341 cactctgaat cgaaaaaaag gcagttgctt ttcataattc ctgaagatct tttgagaaaa
     2401 tcgagtttgc gatatttcaa gagctggttg aacgacattg tttttataac ttccagctca
     2461 ctttcagtaa ccttcattgc aagagacact tcccctttct ctttttctat cctctccatg
     2521 agtatttttt ctttgagcgt tacaggtcta ttctgagaaa acagcatcaa cgatgacaaa
     2581 ataaaaacta atgaaattaa tatcctacag aatctcttca tagacgaata caagttctcc
     2641 catgaatttt atattatgaa aatcttttgc ataggcaggt gtccaggcag taaaatcatc
     2701 cgatttcgac tttttgccgg tatgcctgtt tatacggtat aaaaacactt tccatgtatc
     2761 accgtctttt ggcggattta caggagcatc ttcaattgaa ctccacggaa ttgcgatctc
     2821 tgcacgccat cccttatcct tttcattgct gtcattcaac ttaccgtcaa ctttaaccgc
     2881 atatttgatc tttgaatccc aactgtcgtc acggttcttt cttctgccgt tgaactttga
     2941 atcaaattta acgcctgccg ctgaaacctg catttcgtaa tagattttcc cttcacccct
     3001 gggatcgatg aatatctcaa caacatcatc tgtatcatac agcggatcat cattgttctt
     3061 ctgctcggca tagatatcgc tgtcttcagc atcaaagccg atatacagat agttgtggtc
     3121 cattaccgcc atgacagagg tttttacaga actcagacct tcaccgcttg agatccagaa
     3181 tttatcatct tttgatgcat tggcccagta actctccttg aactcgccgt caacttttgt
     3241 ctgttttctt gaatttccgg cgatcgtata gacctccatc ttctttctta aaatgttggg
     3301 ctttgttatc tttatctgtg ccatgttaag acggttcttc ccgtcattga actgtttttc
     3361 aggttttgtt ctctcttttc ccttgaaaaa tccacctctt acaaacattg tatctgtgtc
     3421 aaaatgtctc ggaagttcct taattaccgc ctcatcctta attatctttc caagagcgag
     3481 ctttttcact tttccgtcaa taaattcatg gtcatattta aaatgctctt cacctttttc
     3541 atcctcaaaa tggtaaaaga gtttccaacc gtcgccaagc tgttcatcaa acttccagaa
     3601 agttactatc ttaaagcttt ctccatgcct gggtttgatt ggttcagtat aaaaacccat
     3661 aaattccact ttcccgtcat tgagcttcat tcccgttttt attgcatttt tcggcagttc
     3721 tccgacaact ccttcatggg ttgaacatga agaaagaata aaaaacataa cgataaccat
     3781 taaaagtttc ataaaaccct ccta
//
LOCUS       DAOOYB010000279         3798 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_28700_length_3798_cov_14.155757, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000279 DAOOYB010000000
VERSION     DAOOYB010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28700_length_3798_cov_14.155757"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1418)
                     /locus_tag="PL056_12120"
     CDS             complement(<1..1418)
                     /locus_tag="PL056_12120"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HPM47809.1"
                     /translation="MQLVKISGLYFKYPESVDFVLENVNCEVGRGDRIGIVGHNGGGK
                     STLLKLIKGMIEPSDNGLLINDECSVAYLPQDYSGLENEIGTKDAVLMSGGEKVKFLL
                     RKMILDNPDLILLDEPTNHLDIKGIEWLEKFINKTSSAVIIISHDRSILNNCVNETWE
                     IDMGKLTIFKGNYDTYEKLKFEESARRQKAYSEKKDQVKRLETAVRDRKNRAEKNEKF
                     KPSRSVKKNGGLCQYDMGSIKSVRSGNLMKSAKALDSRIEKERELMYELKESIAKKAK
                     VQINSGGSKNRFIVKLFDISKSFGEKQVLSRFSLSVERGEKIALTGPNGCGKSTLLKI
                     IAGEISQDSGEISISPSTKTGYFAQGNAELPHDRTIIDFLADGNREIENRARLFLGCL
                     NIKGDTVFRKIGTLSAGQISKVEIASFMLGEYDLIILDEPMSHLETNTRNALEEALRN
                     FEGAAIVVTHDRTFAQNFADKIV"
     gene            complement(1609..2184)
                     /locus_tag="PL056_12125"
     CDS             complement(1609..2184)
                     /locus_tag="PL056_12125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47810.1"
                     /translation="MKISKMKFYGGSREKVCRLGLSHIFLELQEMLISTEILILKKKQ
                     ANGAGRLRELIDEKFKSYDDWILSKSGDIDWIKKIRYNHSIISRIGVEIQVSGRSDLL
                     ARDIVHIRNNLQDSNIDVGVIVVASDEFEYYLTDRIANFSYAVRYVEEELREAQSYPI
                     ILLAIEHDGFSDTPLPKKITNTGKQKAEKAD"
     gene            complement(2105..2995)
                     /locus_tag="PL056_12130"
     CDS             complement(2105..2995)
                     /locus_tag="PL056_12130"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015939245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HPM47811.1"
                     /translation="MTVPYFKTSHGVLYNDDCLNILAKMKANVVDTVFADPPFNIGKD
                     YKNGYNDDINHCDYMAWCKQWILECSRVLKPGGAFFLYATPELAVQFAPMMGELLSFR
                     HWIAVSMKGSFHRGNKLYPAHYALLYYTKGDPKVFNKLRLPIEICRHCGGEIKDYGGH
                     RSKMNPEGVNLSDIWTDTSPNRHKKFKVRPGVNELKMIIPERAILMSTEEGDLVFDPF
                     GGGGSTFQAAEKHHRNWIGTELYDSEHIKKRIEENYSTSALVEPRFNFKELFNEDKQN
                     EILRRVKGKGLPLRAFTHIS"
     gene            3228..3797
                     /locus_tag="PL056_12135"
     CDS             3228..3797
                     /locus_tag="PL056_12135"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006313319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HPM47812.1"
                     /translation="MSYFKKLVGEKCYLSPIRMEDAEIYTKWFNDMEMVLYVAPHPSV
                     ISLFKEKEFIENQLKSGAPIFGIIDIKTDKIIGNTSLMDINQINGTATLGIVIGEKDY
                     WDKGYGTEAVKLILDYGFNILNLHNIMLCVFDFNKRAVASYKKIGFKEIGIRREDRLI
                     ARHRHNSVFMDILATEFESPFIKGIADKL"
BASE COUNT         1111 a          788 c          647 g         1252 t
ORIGIN      
        1 acaattttgt ctgcaaagtt ctgtgcgaat gttctatcgt gggtgacaac gatggcagct
       61 ccttcaaaat tacgcagtgc ttcttcaagg gcgtttcttg tgttggtttc aaggtgagac
      121 atcggttcat caaggattat gaggtcatat tctccaagca tgaacgaagc aatttcgact
      181 ttgctgatct gtcctgcact gagtgtgccg atctttctaa acactgtgtc gccttttata
      241 ttgagacagc caagaaaaag ccgtgccctg ttttcaattt ctctattgcc gtcggcaagg
      301 aaatcaatga tcgttctgtc atgaggaagc tcagcatttc cttgagcaaa atagccggtc
      361 tttgttgatg gagagattga aatttcaccg gaatcctgag aaatttcacc ggcaatgatc
      421 tttaatagtg ttgattttcc gcaaccgtta gggccggtca gagcgatctt ttcacctcgc
      481 tcaacagaaa gagaaaacct gctcagaacc tgcttttcac cgaatgattt tgaaatatca
      541 aaaagcttaa ctataaatct gttcttagaa ccgcctgaat ttatttgtac ttttgctttt
      601 ttagcaatgc tctcctttaa ttcgtacatc agttcccgct ctttttctat ccttgaatca
      661 agagcttttg cacttttcat caagtttcca gatcgcacag attttatgct tcccatatcg
      721 tactgacaga gtcctccatt ctttttcact gaccttgacg gtttgaattt ttcatttttc
      781 tcagctctgt tctttctatc tctcacagcc gtttcaagcc gttttacctg gtctttcttt
      841 tcagagtaag ctttctgtct gcgagcagat tcctcaaatt ttaacttctc ataagtatcg
      901 taatttcctt tgaaaatggt taacttcccc atatcaattt cccatgtttc attgacacag
      961 ttattcaaaa ttgatctgtc gtgacttata atgataactg cggatgaagt tttgtttatg
     1021 aacttttcga gccattcgat cccttttatg tcaaggtggt ttgtcggttc atcaagaagg
     1081 atcagatccg gattatcaag aatcattttc ctaaggagaa attttacttt ttctcccccg
     1141 ctcatcaaaa cggcatcttt agttcctatc tcattttcaa gacctgaata atcctgagga
     1201 agataggcga cagaacactc atcgtttatt aaaagtccgt tatcagacgg ctcaatcatt
     1261 ccttttatca gctttaacag agttgatttg cctccgccgt tatgacccac tattccgatc
     1321 cggtcaccgc gacccacctc gcaattcaca ttttcaagaa caaaatccac actttccgga
     1381 tatttaaaat aaagccctga aatttttaca agttgcataa gttacctcaa aaaagatgtc
     1441 attaacaaca aaagaatatt tttgaaactt agattctcat cggaccatac acctcaaatt
     1501 caaggtacct cgttcatatt aaagagaatg ttcgtgatgt caagagtcaa gaaaaatctg
     1561 tcatattact tcaatctatg aatgagaata tttatttttg tttttttatt agtccgcttt
     1621 ttcggctttt tgcttgcctg tgtttgtgat tttcttggga agcggtgtgt ctgaaaaacc
     1681 atcatgttca atagcaagca gaattatcgg ataagattgt gcctctcgca actcttcctc
     1741 aacatagcgt acagcatagc tgaaatttgc tattcggtcg gttaaataat attcgaactc
     1801 atctgaagca acaacaataa ctccaacatc aatattgctg tcttgaaggt tgtttcttat
     1861 gtgcacaata tctcttgcca gaagatcgct cctgcctgac acttgaattt ccactccaat
     1921 tctggatatg atagaatgat tgtagcgaat cttttttatc caatcaatgt ctccggactt
     1981 tgataaaatc caatcatcat agctcttgaa tttctcgtct ataagttctc ttaaacgacc
     2041 agctccgttt gcctgcttct tttttaaaat tagaatttcc gtagagataa gcatctcctg
     2101 taattcaaga aatatgtgtg aaagccctaa gcggcaaacc ttttcccttg acccgccgta
     2161 aaatttcatt ttgcttatct tcattgaaca gctccttaaa attaaacctc ggctccacta
     2221 aagcggaggt tgagtaattt tcttcgatac gcttttttat atgttccgaa tcatacagtt
     2281 ccgtaccgat ccagtttcga tgatgttttt ctgccgcttg aaatgtagac cctcctcctc
     2341 caaagggatc aaaaacaaga tctccttctt cagttgacat taaaatagct ctttccggta
     2401 taatcatttt taattcattt acgccaggac gaactttaaa ttttttatgt cgattgggcg
     2461 atgtatctgt ccaaatatct gaaagattta ctccttccgg attcattttt gatcgatgtc
     2521 caccataatc ttttatctca ccgccacagt gtcggcaaat ctcaattggc aaacgcaatt
     2581 tattaaatac cttaggatct cctttagtat aataaagaag agcatagtgt gctggataaa
     2641 gcttatttcc tctatggaaa gagcccttca ttgaaacggc tatccaatgt ctaaaactca
     2701 acaattctcc catcatcggg gcaaattgga cagccaattc aggtgttgca taaagaaaaa
     2761 aagcaccgcc aggtttcaat actctggaac actcaagtat ccactgctta caccaagcca
     2821 tataatcaca gtgatttatg tcatcattat atccattttt gtaatctttt cctatattaa
     2881 agggcgggtc tgcaaacacg gtgtcaacaa catttgcctt catttttgcg agaatgttta
     2941 agcaatcatc attgtaaaga acgccatgtg atgttttaaa ataaggaact gtcactgaaa
     3001 catccgtatt atatttggac actttttact cctagacaag cactcatact attgtttatt
     3061 tcaatcctga atgatctttt tttactatac agcaacttcc tgttttttcc aaataatttc
     3121 aaattacaaa tgagaaagcg gttatgtttc aaacatcata aaaattgaca gtctgctgat
     3181 gcacagctac tatccggcga atgaactgaa atttcaggag gaaaaaaatg agttatttca
     3241 agaagctggt tggtgaaaag tgctatttgt ctccaatcag aatggaagat gcggaaattt
     3301 acacaaaatg gtttaacgac atggaaatgg ttctttatgt tgctccgcat ccgagtgtta
     3361 tttcactttt taaagagaaa gaattcatag aaaatcaact gaaatcgggt gctccgatat
     3421 ttggaataat tgatataaag acggataaaa ttataggcaa cacttctctc atggatatta
     3481 accagattaa cggcacagca actcttggga tagtaatagg tgaaaaagat tactgggaca
     3541 aaggatatgg cacagaagct gtaaaactga ttcttgatta tggctttaat atcttgaatt
     3601 tacacaacat tatgctgtgt gtctttgatt tcaataaacg agcagtagca tcatacaaaa
     3661 agatcggatt caaagaaatt ggaatccgca gagaagacag acttattgca agacaccgtc
     3721 ataattcagt tttcatggat attctcgcaa ccgaatttga atcacctttc atcaaaggaa
     3781 tcgctgacaa gctttaga
//
LOCUS       DAOOYB010000280         3795 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_28744_length_3795_cov_6.835294, whole genome shotgun sequence.
ACCESSION   DAOOYB010000280 DAOOYB010000000
VERSION     DAOOYB010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28744_length_3795_cov_6.835294"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="PL056_12140"
     CDS             complement(<1..197)
                     /locus_tag="PL056_12140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47813.1"
                     /translation="MLQNSFIKILVFAVAASILYGCSNEYQIESFDYKAEYDRVSKTK
                     SSEVKAKKEIDLTDIAQRKSN"
     gene            complement(184..840)
                     /gene="pilO"
                     /locus_tag="PL056_12145"
     CDS             complement(184..840)
                     /gene="pilO"
                     /locus_tag="PL056_12145"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HPM47814.1"
                     /translation="MKALLVNIGKLKPAYKYGGLLGILIVIMLAYYITVYMPHLDKME
                     SLFGEFNAKKAEFSKMVVLVRDRGQFMEEVEALDRDLKVAISMLPDKKEIPSLLKKLS
                     DEAEKFGLEVYYFEPLAERKKDFYAEVPVSIKVKGSFHEVLGFFDSVNKLARIVNVSD
                     IDIKADTQKTQTKQVKGNVVSAFSGFQPNKTYLDIMFRATTYRFLTSAEMGGGANAAK
                     "
     gene            complement(861..1493)
                     /locus_tag="PL056_12150"
     CDS             complement(861..1493)
                     /locus_tag="PL056_12150"
                     /inference="COORDINATES: protein motif:HMM:NF016992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HPM47815.1"
                     /translation="MIRINLLPVKEEKLINEAKGFLVIFVISIALVVVLVVLNSAVLA
                     EREAESRMRIDEADKEIANLKSIMGEIQTLKEKKAKLQEKMDMIVKLQEQNIGPVRVL
                     DELSLKIPSNKIWVEKISLKGDKINMEGKTLENQEVANLMKQLENSLFFSGIELKKVT
                     KDKVVEGVPILSYGLNTVIHYAGRQVQPAAPAPATAPSAETVPAQGNATN"
     gene            complement(1490..2542)
                     /gene="pilM"
                     /locus_tag="PL056_12155"
     CDS             complement(1490..2542)
                     /gene="pilM"
                     /locus_tag="PL056_12155"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HPM47816.1"
                     /translation="MFYNKQLLGLDLGSYLIKASRLQKKRGKYYLKKFGFIPIPVNTV
                     VDGSIMNTYEMNDAIKTLLARERLKDKFCSICIAGHGIINRIINVPKVTKEEFYNALK
                     VEAETHIPHDIKEIYFDGLKTDIVEDGRDRVVLIAARKDLVGDFIQVVNDAGVRPMSV
                     EIDATALANVFEINYPEEKDKTVAILNVGASKINVVIIANGNVRFFRDILTGGNYITE
                     EITRRLKVSFQQAESLKSGSHTTGDSILPNQVEEVVADVAKNMVADISRVFDYYTNTN
                     PEDSVTKVFITGGTTRSHTFTQTLRSTLEIPVEKLNPFKEIIVPGQVLNREEVEDYAH
                     IATISLGLALRRMDEQ"
     gene            complement(2558..2767)
                     /locus_tag="PL056_12160"
     CDS             complement(2558..2767)
                     /locus_tag="PL056_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPM47817.1"
                     /translation="MAYNNLKKMREKLLMSKSELARKAGVSVLTIDRIEKNGECRMET
                     KRKVLLALGLSLDEKHKVFSDDDED"
     gene            complement(2911..>3795)
                     /locus_tag="PL056_12165"
     CDS             complement(2911..>3795)
                     /locus_tag="PL056_12165"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF006873.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HPM47818.1"
                     /translation="GATYSHSTNGYNLSANRLKGADIFLDEASVTATENIVMAAVTAE
                     GVSVIRNAATEPHVQNLCRFLNKMGAKISGLGTNVLRIEGVTSLKGCEHTIGPDYIEA
                     GSYIALAAMTGGELLVKDAAGGNDLRSILNVYKRIGITTVAKGNDIFVPHHPKLLIQK
                     DMNNAIPKVDDGPWPSFPSDLMSIAIVAATQSEGTVLFFEKMFESRLFFVDRLIGMGA
                     NIILCDPHRVVVSGRTLLSGSKLDSPDIRAGMALLMAAISAQGRSRIYNIKQIDRGYE
                     SIEHKLTRIGVTINRSKA"
BASE COUNT         1004 a          897 c          676 g         1218 t
ORIGIN      
        1 taattcgact ttctctgagc gatatcggta agatctatct ctttcttagc tttgacttca
       61 cttgactttg ttttgcttac acggtcatat tccgccttat agtcaaagga ttctatctga
      121 tattcattag aacatccgta aagaatggat gctgcaacag cgaaaacaag tatttttatg
      181 aaactatttt gcagcatttg cacctccacc catttcagca gatgtaagga atctataagt
      241 ggttgcccta aacataatat caagatatgt cttattgggc tgaaaaccgc tgaaggcact
      301 tacaacattc cctttcacct gtttggtttg agttttctga gtatcagctt tgatgtctat
      361 atcgctgaca ttgactattc ttgcaagttt attgacactg tcaaaaaacc ccagaacctc
      421 atggaaactg cctttaactt ttattgatac cggaacttct gcataaaaat ccttctttct
      481 ttccgcaagc ggttcaaaat aataaacttc cagtccgaac ttttctgctt catcagaaag
      541 ttttttaagt agcgaaggaa tctctttctt gtcaggaagc atactgatcg caactttgag
      601 atctctgtca agggcttcca cctcttccat aaactgacct ctgtcgcgaa cgagaactac
      661 cattttggaa aattcagcct tttttgcgtt gaactcaccg aaaagggact ccattttatc
      721 aagatgcggc atataaacag taatgtaata tgcaagcatg ataacaataa ggatcccaag
      781 aagtccacca tacttatagg ccggtttcaa ttttccgatg ttaaccagca gggccttcat
      841 atcgtactcc tcaacagtta ttaattagtc gcattgcctt gtgcaggaac tgtctctgca
      901 gaaggtgccg tagcgggtgc aggtgctgca ggctgtacct ggcggcctgc gtaatggata
      961 accgtgttca aaccatagga aagtatcgga actccctcta caactttatc ctttgtaacc
     1021 ttcttcagct ctatgcccga aaagaacaga gaattttcaa gctgtttcat caagttcgca
     1081 acttcctgat tctcaagggt ttttccttcc atattaattt tatccccttt taaagagatc
     1141 ttttccaccc atatcttatt ggacggtatt tttaatgaaa gttcatcaag aaccctgacg
     1201 ggaccgatat tctgctcctg aagcttcact atcatgtcca tcttttcctg taattttgct
     1261 ttcttttcct tcagggtctg aatttcaccc attatcgatt taagatttgc tatctccttg
     1321 tcagcttcat cgatcctcat tctgctctcg gcttcccgtt cagcaagcac agcgctgtta
     1381 agaacaacaa gcacaacgac caacgcaatg gaaatgacaa agattacaag gaagcctttc
     1441 gcctcattga tcagcttttc ttcttttacc ggtaaaagat ttattcttat cattgttcgt
     1501 ccatcctccg aagtgccagt cctaaactaa ttgtcgcaat atgtgcgtaa tcttcgactt
     1561 cctctctgtt aagaacctgt cctggaacaa ttatctcttt gaaaggattc agcttttcaa
     1621 caggaatttc aagagtggac ctgagtgtct gtgtgaaagt atgacttctg gtggttccac
     1681 ctgttatgaa aactttcgta acactgtctt ccggattagt atttgtataa tagtcaaaaa
     1741 ctcttgaaat gtcagcgacc atattctttg caacatcggc caccacttct tcaacctgat
     1801 tgggaagtat ggaatcacct gtagtgtggg aaccggactt caaactttcg gcctgctgga
     1861 aacttacttt cagccgcctt gttatctctt ctgttatata gttccctcca gtaagaatgt
     1921 ccctgaagaa tcttacattg ccgtttgcta ttatcactac atttattttt gaagctccga
     1981 cattaagtat cgcaacagtc ttgtcctttt cttccggata atttatctca aaaacattag
     2041 ccagagcggt ggcatctatt tcgaccgaca tcggtctgac acctgcatca ttaacaacct
     2101 gaataaagtc accgacaaga tcctttcttg ccgcgataag aacaaccctg tcccttccat
     2161 cctcaacaat gtcagttttc agaccgtcga aatatatttc cttgatatcg tggggaatat
     2221 gtgtttcggc ttcaactttc agagcattgt aaaactcttc ttttgtaacc tttggaacat
     2281 ttataatacg gttaatgata ccgtgacccg caatacagat ggaacagaat ttatctttca
     2341 gccgctctct ggcaagaagc gtttttatgg catcattcat ttcgtaagta ttcattatgc
     2401 ttccatcaac aactgtattg acaggaatag gaatgaatcc gaatttctta agatagtatt
     2461 tccccctttt cttctggagt ctggaggctt tgatcagata ggatccgagg tcaagcccga
     2521 gtaattgttt gttataaaac attcgtgcct cctttggtca gtcttcgtca tcatcagaaa
     2581 aaactttgtg tttctcatca agagaaagac ccagtgcaag gagaaccttc ctttttgttt
     2641 ccatcctgca ctcaccgttt ttttcgatcc tgtcgatagt aaggacagaa accccagctt
     2701 ttctcgcaag ttccgacttg ctcataagga gtttctctct catttttttt aggttgttgt
     2761 aagccatcta tcttctccta ataaacctga aaaacatcct ggtaactacc tcgacaatat
     2821 caaaaaacaa cttaaaatca agttttttct gcaaaaaata tacatcattg ttatataatt
     2881 atgcaaaact ttgcaataac ttcactcctt ttaggcctta cttctattaa ttgttacacc
     2941 aatcctagtt aacttatgct ctattgattc atatcctcta tctatttgct ttatgttgta
     3001 gatgcgactt ctaccctgcg ctgaaatcgc tgccatcaga agagccattc ctgctcttat
     3061 gtcaggagaa tccaattttg aaccgcttag caatgtccgg ccggaaacga caaccctgtg
     3121 cggatcacag agaattatgt tggcgcccat gccgataagt ctgtcgacaa agaacagacg
     3181 gctttcaaac attttttcaa agaagagtac cgtcccttcc gactgtgttg cggcaacaat
     3241 tgcaattgac atgaggtctg aagggaacga aggccacgga ccgtcatcaa ctttcggaat
     3301 cgcattgttc atgtctttct ggatcaacag cttcggatga tgaggaacaa aaatgtcgtt
     3361 ccctttcgca acggtggtta tgcctatcct cttatataca tttaatatag agcggagatc
     3421 gttcccgccg gcggcatctt ttaccagaag ttctcctcct gtcattgctg caagtgctat
     3481 atagcttcct gcttcgatgt aatccggacc tatcgtgtgt tcgcatcctt tcagagaggt
     3541 cacgccctct attctgagta cattagtccc cagccctgat attttcgcac ccattttatt
     3601 aaggaaccgg cacaggttct ggacatgcgg ttcagtagct gcattcctta tcaccgaaac
     3661 tccctctgcg gtaactgccg ccatgacaat attctctgtc gccgtaacac ttgcttcatc
     3721 aagaaatata tctgccccct taagtcggtt tgcagaaaga ttatacccgt tggtgctatg
     3781 actgtatgtt gctcc
//
LOCUS       DAOOYB010000281         3731 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_29448_length_3731_cov_12.929815, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000281 DAOOYB010000000
VERSION     DAOOYB010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3731
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29448_length_3731_cov_12.929815"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(136..804)
                     /locus_tag="PL056_12170"
     CDS             complement(136..804)
                     /locus_tag="PL056_12170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47819.1"
                     /translation="MKKKILFLIIFAVFSNTYAENLDRHRFGISIEGGYSFSMKSSDH
                     KEKQAPEIFKGNFGGTLFYQFTIHKYFAIRPEVGLYSYFFGLPVDYSGADYFSTYGVT
                     YAALNGRIGVGLMAYYYRNKSSNFALNFALTPFISMRFYDSANLYKNRKNEDIYEELG
                     LKNERLYYFSGGIIIAAGIETNEVKKVGAGGSLFMRFGDRSTEEGDKILLVPSFGGTV
                     SIFF"
     gene            942..2570
                     /locus_tag="PL056_12175"
     CDS             942..2570
                     /locus_tag="PL056_12175"
                     /inference="COORDINATES: protein motif:HMM:NF018449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HPM47820.1"
                     /translation="MRNALIFLMVLSMFSCSLDKFEKNSPDSDSISVQSDTDASENDN
                     NTDELEKTDDFMPENEQNDEGADEKDRKDDIIVENDETPDLFKDDFEPDDTEETDDSN
                     EPSTDIDTQTSDAEPDDKESVDTETDDDIAENPDEDLLEPYKNFTTILFGTDKNEYGL
                     SLAMDNDGNLFLTGYTEGNLDGQTNSGESDIFLIKFDNYGNKLWTKLYGAEKNDIGRT
                     VKTDSSGNIYITGYTEGDLDGNGNKGSYDAFLMKLDQAGEVLWVEQIGTEEADVSMSI
                     VFDETENIYISGYLDGSYDDPPSIGTPDAFLAKFSQDGTPLWLKEWGSENFDSGAIAS
                     FHDGYIYVPGFTNGDINGTGFLGGIYDAFLTKLDTDGNIIWTKQLGTSGMESVYSLEF
                     DSNSNMFLCGSASGEMENPDLNDSGVSLTKLDSDGNFKWTNIWNSGKWEGSNYIISDS
                     SDHFYLSGFSAGNLDGYKNAGEMDVVLMKTDSFGNKLWTKLIGGENIEISYGSIMDMY
                     GNIYITGTTMSDLGGNKNAGETDIFLVKINAEDL"
     gene            2586..3092
                     /locus_tag="PL056_12180"
     CDS             2586..3092
                     /locus_tag="PL056_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47821.1"
                     /translation="MRNALIFLMVLMVLSSCSDSNLAENVKTSDCGGFESGIVTKLPG
                     GEEEDCSQKLVWSYDESSKILNIINEAVNLNCCGEHAININETGENEYEYIMVDNADR
                     GMRCGCDCLFDFGADISGIDNSSVKLKVYADIEEEAEKTLYWEGTLDMTQKSGTIVVK
                     ERTGDGCY"
     gene            complement(3156..>3731)
                     /locus_tag="PL056_12185"
     CDS             complement(3156..>3731)
                     /locus_tag="PL056_12185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47822.1"
                     /translation="DRHRYGFSLEGGASFTFLSGRANNTKPWHDLYGSAGGTVFYNYK
                     ISNRFAIRPELGLYSYFFAIPGPGDIGNGTEATLNGRLGLTMIFYTYVGIDASFPLGL
                     TTFISSRFYDSAGKYKSRLSEEENDVYKEILKNDYLSGGVQISFGVESTRSEPMGAGF
                     SIFTRFADTFSTGGQSTSIIPTFGAFLVIYF"
BASE COUNT         1252 a          647 c          793 g         1039 t
ORIGIN      
        1 tgtcgcttcc gcatgaaact gcaaaaacaa gggaaacgat cataattgca aaaataattt
       61 ttttcataac tcctcgcttg aaaaattgat cacacacgca gaattttaag gacttgtcag
      121 tttttagtca aatatttaga aaaatatcga cactgttccg ccaaatgacg ggacgagcag
      181 tattttgtct ccttcttctg tcgatctgtc accaaatctc atgaaaagag atccgccggc
      241 tccaactttt tttacttcat ttgtttcaat accggcagcg ataattattc cgccggaaaa
      301 atagtacaac cgttcatttt tcagaccgag ctcttcataa atatcttcat tctttctatt
      361 tttgtaaagg ttggcagaat cgtaaaatct catggaaata aaaggagtta aagcaaaatt
      421 aagtgcgaag ttactgcttt tgtttctata atagtaagcc ataaggccga caccgatcct
      481 gccgttgagt gcggcataag tcactccgta agttgaaaaa taatcggcac cgctgtaatc
      541 aacaggaagc ccgaaaaaat agctgtaaag cccaacctcg ggacggatcg caaaatattt
      601 atggatcgta aactgataga acagtgttcc gccgaaattc cccttgaaaa tttcaggagc
      661 ctgcttttct ttatgatcgc tgctcttcat tgaaaaagag tagccccctt caattgatat
      721 cccgaacctg tgacgatcga ggttttcagc gtaagtgttt gaaaaaacag caaatattat
      781 taaaaacaaa atcttctttt tcactataac cctttttcac cgattgagcg gtagatataa
      841 tatttcattt gtttgaacga atcaattttt taatcaacct gattgacaat tatttgtttt
      901 agaagtataa attagcggtg ttcagaaaaa cggagagaaa aatgagaaac gctttgattt
      961 ttttgatggt tttatcaatg ttttcgtgct ctcttgataa atttgaaaaa aacagccctg
     1021 attcggattc gatttcagtt caaagcgaca ctgacgcatc agaaaatgac aacaatactg
     1081 atgagttaga aaaaacagat gatttcatgc cggaaaacga acagaatgat gaaggtgctg
     1141 atgaaaaaga ccggaaagac gatattattg tggaaaacga tgaaacgccc gatctgttta
     1201 aagatgattt tgagccggat gacactgaag aaaccgatga tagcaatgaa ccgtcaactg
     1261 atatcgacac tcagacgagc gacgctgagc cggatgataa agaatcagtt gatactgaaa
     1321 cagacgatga catcgctgaa aatccagatg aagatctgct ggaaccatat aaaaacttta
     1381 caaccattct gttcggaact gacaaaaatg aatatggact ttcccttgcg atggataatg
     1441 acggaaattt atttctaacc ggctacacgg aaggaaatct ggatggtcag acaaactcag
     1501 gagaaagcga catattctta ataaaattcg ataattatgg gaacaaactg tggacaaaac
     1561 tttatggtgc ggaaaaaaat gatataggaa gaactgtaaa aacagactca tcgggaaata
     1621 tttatattac cggctacaca gaaggtgatt tggacggaaa tggcaataaa ggcagttatg
     1681 atgcgttttt gatgaaactt gatcaagctg gcgaggtttt atgggttgaa caaattggaa
     1741 ctgaagaagc tgatgtgagc atgagcattg tttttgatga aactgaaaac atctatattt
     1801 ccggatacct tgacggttca tatgatgatc ctccatcgat aggaactccc gatgcatttt
     1861 tagcaaaatt ttctcaagat ggaacaccct tgtggttaaa agaatggggc agcgaaaatt
     1921 ttgattctgg tgcaatcgcc tcttttcatg acggatatat ttatgttcca gggtttacca
     1981 acggggatat caacggaacg ggatttcttg gcggaattta cgatgcattt ttgacaaaat
     2041 tagatacaga cggaaacatt atctggacaa aacagctggg aacaagcggc atggaatctg
     2101 tatattcgtt ggaatttgac agtaattcta atatgtttct atgtggaagc gcaagtggag
     2161 aaatggaaaa tcccgatctt aatgacagtg gcgtttcttt aacaaagctt gacagtgatg
     2221 gaaactttaa atggacaaat atctggaaca gcggcaaatg ggaaggttca aattatatta
     2281 tttctgacag cagtgaccat ttttaccttt ctggtttttc ggctggaaat ctcgacggat
     2341 ataaaaatgc aggggaaatg gatgttgttt taatgaaaac agatagcttc ggcaacaagc
     2401 tctggacaaa actgataggt ggtgaaaaca ttgaaatttc atacggaagc attatggata
     2461 tgtacggcaa catttatata acaggcacca caatgagcga tcttggcggc aataaaaacg
     2521 caggagaaac cgatattttt cttgtcaaaa taaacgcgga agatttatga aaaacggaga
     2581 aaaaaatgag aaacgctttg atttttttga tggttttaat ggttttatca agttgcagcg
     2641 attcaaacct cgcagaaaat gttaaaacaa gtgactgtgg cggatttgaa agcgggattg
     2701 ttacaaaact gcccggtggt gaagaagaag attgcagtca gaaacttgtc tggagttatg
     2761 atgaaagcag caaaattcta aatattatta atgaagctgt caatctgaac tgttgtggtg
     2821 aacatgccat aaacattaat gaaactggcg aaaatgaata cgaatatatc atggttgata
     2881 atgcagacag aggaatgaga tgcggctgtg attgtctgtt cgattttgga gccgatattt
     2941 ctgggattga caattcttca gtcaaattga aagtttacgc tgatattgaa gaagaagcgg
     3001 aaaaaactct ttattgggaa ggaacccttg atatgactca aaaaagcggc accatcgttg
     3061 tgaaagaaag aaccggtgac ggatgttatt aacctaagaa aaaaacgatg aaaaaatgtc
     3121 cgttctgtgc caaaaagatt caggatgata caattctaaa aataaattac taaaaatgct
     3181 ccgaatgtgg gaataatact agttgactga ccgccggtag aaaatgtgtc ggcaaatctt
     3241 gtaaatattg aaaatcctgc tcccatcggt tctgatcggg tgctttcgac tccgaacgat
     3301 atctgtactc cgcctgaaag ataatcattt ttaagtatct ctttgtagac atcgttttct
     3361 tcttcagaaa gacgggactt atattttcct gccgaatcat aaaatcttga agaaataaaa
     3421 gttgtaagtc caagcgggaa agaagcatca attccgacat aagtatagaa tatcattgtg
     3481 agtccaagtc ggccgttcaa tgttgcctct gtgccgtttc caatatctcc gggacccgga
     3541 atagcaaaaa aataggaata aagtccaagt tccgggcgga ttgcgaacct gtttgaaatt
     3601 ttgtaattat aaaaaaccgt tccccctgct gagccgtaaa gatcgtgcca cggttttgtg
     3661 ttgttggctc ttccactgag gaaagtgaaa gacgcaccgc cttcaagcga aaaaccgtag
     3721 cggtgacgat c
//
LOCUS       DAOOYB010000282         3729 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_29473_length_3729_cov_5.646979, whole genome shotgun sequence.
ACCESSION   DAOOYB010000282 DAOOYB010000000
VERSION     DAOOYB010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3729
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29473_length_3729_cov_5.646979"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            412..2208
                     /locus_tag="PL056_12190"
     CDS             412..2208
                     /locus_tag="PL056_12190"
                     /inference="COORDINATES: protein motif:HMM:NF012570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HPM47823.1"
                     /translation="MFLKSISDERLAKIASLNLKFMGEKEEVFKNRLIEGEKHLDIVV
                     AGQFNAGKTTFLNALLMLESEKELPTSSLPLTSSPWKLQWSPKTEDLVNVITIDGKKE
                     GKDWDELKKIQKSPIQNSIDHFEAFIASDVLNIENCIFWDTPGFNDPKKDDSKTLKFL
                     SDHEVIIFVTDYQNYKHKIKVNDEEKEIIGHILSPINSKCIVILTNSDKNENFFEKKE
                     DYCKEIEEHYKKYDRKIKVLPLESKKALSSQRELKKTVDEMSAAVNSFLKNKNINLYG
                     ETDWTELVKKNSDDVKNSGIIEVRQAVSNITENKEILIVKSGIERIAREVEKIEKKID
                     KVLQQQKDEFEKVKKEKDLFIETLNPPKDEDLFNNETVVQKASSKIVRFLPEYFAEYK
                     NNLKDKVQIVITTSKPWFYGWVKDMNKLAESEIPKFFKEQTKKIRTAMEEQYKEIFKK
                     VLKDDLSKYAKKNNMNDDFMKETDILLEKQTLKKILDEISQSTSNQCLKKIKEKIDNK
                     EKLKTPPLDEISKEVSDIFQKSLEKEIEEISKKVFPKYSETRTELKYEMEKKVKEDNS
                     EELKRLEQIVAECNNKLNETKNDLSVLIDKKG"
     gene            2231..>3729
                     /locus_tag="PL056_12195"
     CDS             2231..>3729
                     /locus_tag="PL056_12195"
                     /inference="COORDINATES: protein motif:HMM:NF012570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HPM47824.1"
                     /translation="MQNESKIFSLRKTQYLSLIDEINQKVTDKLSSKKLIKNQLEESK
                     SKITNEEFLIAFVGDWSSGKSSLINKILFDEYSQKGVDFLPVLDERTTTRITRIQYGE
                     KTKMFILKNDGSKELLADGEEKVMAALKEHASTQGSKSEKDSGEYRLLIEHPAEILRS
                     GIVFVDTPGLQDEDHSSITEDEMHYVDAVVLVFPFNLPFTQAMDDFLQKRVWRSSIQK
                     IFFLLNKCDRISTDDDVDKNGRINYTYDTIRDKSQEYAASFREFLTGSKDFLYDDIKK
                     DHFFGVSAKTGEGLAEFCKKLADFTDGQDKNIALYNSAKSRIEMSLSTLRNEISDKEN
                     SIGKDKNVFDRQIEQQNLIQEEIKKQWNQTIQVFNDEYKDFSKKIYNDLETFLKSMVE
                     EAETFFEKEKSFWRPGKTSKKLNEKIAAFNKKIDIKIKRSLREVEEKKEDLFIKLLDR
                     LDSILKEVNFKILQENQQSIEEQDVNANPLIAGTFGGAAIGLSVGAATGA"
BASE COUNT         1527 a          497 c          685 g         1020 t
ORIGIN      
        1 acccggtcaa atttgcctca atgatctctg aattcatgtc ggaagtaccg gtttagaaga
       61 ttgtaagggc aaaagatttt ttgcccctac gccctctctt tcaaaaatag agggaatccc
      121 gtgacgaaag aatctttaaa acctgttcaa aacaatgcaa gtgcctgatt ttaatgataa
      181 ataattaatt tgatttgttg ctgtcaaatc attattataa tttgaatttc aggagggaaa
      241 gatgatagat gcgatggatt ccgctatcgg taaatctgaa gcccgaattt atgcttttta
      301 tggtatggat ataaatcatt taaaacctga caatagtggg aaaagtgcct gaatttgaag
      361 ggataaagag tattattgaa aggttgatag aaacaaattg ggaggattca tgtgttttta
      421 aaatcaataa gtgacgaaag gttggctaag atagcatctc ttaatcttaa gtttatgggg
      481 gaaaaggaag aagtttttaa aaatcgtttg attgaaggag aaaaacatct tgatattgtt
      541 gtagcgggtc aatttaatgc agggaaaaca acttttttaa atgccctttt aatgttagag
      601 agcgaaaaag aacttcctac atcttcactt cctctaacaa gctctccgtg gaagctgcag
      661 tggagcccta aaaccgaaga tttagtaaat gtaataacta ttgatggaaa aaaggaaggt
      721 aaagactggg acgagttaaa aaaaattcaa aaaagtccca ttcaaaatag tatcgaccat
      781 tttgaagctt tcatagcatc tgatgtttta aatattgaaa actgcatatt ctgggacaca
      841 ccgggattta atgaccccaa aaaagatgac agcaaaactc taaaattcct ttcagatcac
      901 gaagtaataa tattcgtgac tgattatcaa aattacaagc ataaaatcaa agttaatgac
      961 gaagaaaaag aaatcatagg tcatattttg agtccaatta actcaaaatg tattgtaatt
     1021 ttgactaact ccgacaaaaa tgaaaacttt tttgaaaaaa aagaagatta ttgcaaggaa
     1081 atagaagagc actacaaaaa atatgaccgc aaaataaagg ttcttcctct tgaaagcaaa
     1141 aaagctttgt catctcaaag agaattaaaa aaaaccgttg atgaaatgag tgcggcggtg
     1201 aacagtttcc ttaagaacaa aaatattaac ctgtacggtg aaactgattg gactgagctt
     1261 gttaaaaaaa attcagacga tgttaaaaac tctggcatta ttgaagttag gcaggcagta
     1321 agtaacatta ctgaaaacaa agaaattctt attgtaaaat cagggataga gagaatcgcc
     1381 agagaggtcg aaaaaataga gaaaaaaata gacaaagtct tgcaacaaca aaaagatgaa
     1441 tttgaaaaag taaaaaaaga gaaggatctt tttattgaaa ctcttaatcc gcctaaagat
     1501 gaagatttat ttaataatga aactgttgtt caaaaggcct cttccaaaat tgttagattt
     1561 ttacctgaat attttgcaga atataaaaac aacttaaaag acaaagttca aatagttata
     1621 acaacatcca aaccatggtt ttacggatgg gttaaagata tgaacaaact tgctgaatca
     1681 gaaattccaa aattttttaa agaacagaca aagaaaatta ggacagcaat ggaagaacaa
     1741 tacaaagaaa tttttaagaa agtgcttaaa gatgaccttt caaagtatgc taaaaaaaac
     1801 aacatgaatg atgactttat gaaagagacc gacatattac ttgagaaaca aaccttaaaa
     1861 aaaattctgg atgaaatatc tcaatcaaca tcaaatcaat gtcttaaaaa aataaaggaa
     1921 aaaatagata ataaagaaaa actcaaaacc ccgcctttag atgaaataag caaagaagtt
     1981 tctgatattt ttcaaaaatc attagaaaaa gaaattgaag aaatttcaaa aaaggtattt
     2041 ccaaaatatt ctgaaacaag aactgaatta aaatatgaaa tggaaaagaa agttaaagaa
     2101 gataattccg aggagttaaa aagattggaa cagatagtag ctgagtgtaa taataagctg
     2161 aatgaaacta aaaatgattt atcggtttta atagacaaaa aaggttgaaa acttaaacaa
     2221 ggagaccatc atgcaaaatg aatcaaaaat cttttcatta agaaaaacac agtatttatc
     2281 tttaattgac gagataaatc aaaaagtaac cgacaagctt tccagcaaaa aattaataaa
     2341 gaaccaactc gaagaaagta aatctaaaat tacaaatgaa gagtttttaa tcgcttttgt
     2401 aggtgactgg agctcaggta agagcagttt aattaacaaa atattgtttg atgaatattc
     2461 tcaaaaaggt gttgactttc tgccagttct tgacgagcgg acaacaacca gaatcacaag
     2521 aatacaatat ggcgaaaaaa caaagatgtt tattttgaag aatgatggtt caaaggaact
     2581 gttggcagat ggcgaagaaa aagtaatggc tgctctaaaa gaacatgcat ccacacaagg
     2641 ttcaaagtca gaaaaagatt caggagaata cagactgtta attgaacatc ctgcagagat
     2701 tcttagaagc ggcattgttt ttgttgacac tcccggatta caggatgaag atcatagttc
     2761 aataacagaa gatgaaatgc attatgttga tgccgttgtt ctggtttttc cttttaatct
     2821 tccattcact caagcaatgg atgattttct gcaaaaaaga gtttggagaa gcagtattca
     2881 aaagattttc tttctattga ataaatgcga cagaatttca acagatgatg atgttgataa
     2941 aaacggaaga ataaattata catacgatac cattagagat aaatctcaag aatatgccgc
     3001 ttcttttaga gagtttttaa ctggcagcaa agacttttta tatgatgata taaaaaagga
     3061 tcactttttt ggtgtttccg ctaaaactgg ggaaggactt gctgagtttt gtaaaaagct
     3121 tgctgatttt acagatggtc aggacaaaaa cattgccctt tataatagtg caaaaagcag
     3181 gattgagatg tctttgtcaa ctctgagaaa tgaaatatct gacaaagaaa attcaattgg
     3241 gaaagataag aatgtgtttg atagacaaat tgaacagcaa aatctgattc aggaagaaat
     3301 aaaaaaacaa tggaatcaaa caattcaagt ttttaatgac gaatataaag atttttcaaa
     3361 aaaaatatat aacgatttag agactttctt aaaaagtatg gttgaagaag cagaaacatt
     3421 ttttgaaaaa gaaaaaagct tctggcgtcc tggaaaaaca tcaaaaaaat taaatgagaa
     3481 aattgcggca tttaataaaa agattgatat aaagatcaag cgctctttgc gggaagtgga
     3541 agagaaaaaa gaggatttat tcattaaatt acttgataga ttggattcaa tattaaaaga
     3601 agtaaatttt aaaatattgc aagaaaatca acagagtata gaagagcaag atgttaacgc
     3661 aaacccatta attgccggca catttggtgg agctgcaatt ggactgagtg taggtgctgc
     3721 gacaggagc
//
LOCUS       DAOOYB010000283         3688 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_29892_length_3688_cov_6.750344, whole genome shotgun sequence.
ACCESSION   DAOOYB010000283 DAOOYB010000000
VERSION     DAOOYB010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3688
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29892_length_3688_cov_6.750344"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="PL056_12200"
     CDS             complement(<1..408)
                     /locus_tag="PL056_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010539018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein beta subunit/FixA
                     family protein"
                     /protein_id="HPM47825.1"
                     /translation="MLKIKVLVKQVPDTANITGNAMKDDGTVNRAVLPAVFNPEDLNA
                     LELALSIKDKLGDCEVTVVSMGPAKAVDVLKHSLFMGADKVYLISDRAYAGADTLATS
                     YVLSEAIKMLGGADIVLAGRQAIDGDTAQVGPQV"
     gene            550..2406
                     /locus_tag="PL056_12205"
     CDS             550..2406
                     /locus_tag="PL056_12205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47826.1"
                     /translation="MSVLKNNSSKYLAAYLFFLCVTTGRFFFEAHYAHFVDTKNLIFH
                     HHYWFLFVFLLFLINFKFFLGMHPSRSWWIAFLSPVIFIPLIYNMIFRGGNLVKFNYL
                     SAKDPDYLLNIFTFMLFSEKNRPVGIELIIVTFSIFLFSYFISKKPVRSAICAVSCYL
                     SLMIFAGTVIIAPHKPDFTLFFINSSFKLQNFMSFVYFSASVTAAVVLFFNEIVVFFK
                     DQRRLICFAVIFTCFFICSQFALINPSSADRFLMIPHSFLFSLSITVLIFINKDFPLK
                     ILLFFISIISSGILFQLFSTLWIQKTPEETQGMNFNDRIEHKIEEGIDILLSRQEQNG
                     EFTSVSCKSPDRKQCSVHKGSVFISTFILYSLNFVKSNSKAQAIVKKGANFVQSKMED
                     GDLWRFWGNRIDYDLDDTSCSSFILELSDKSLLNKDKIYSNMDQKGLFKTWIRESGKN
                     DVDGVVNANVLLYLGENRKTIPACSALVDAVIENNEFSLNYYYPDIAFFYYTLSRAYF
                     EKQMKCIEKGIPVIVTKVENMLNESISDNDVMRTAVLLNTLLNFKEYSNLMDVSAKKL
                     LETNLHEISLAKKPFFVAGEPPDEPSFFYFSNETVIASLIEFFKRYNDLKRN"
     gene            complement(2415..>3688)
                     /locus_tag="PL056_12210"
     CDS             complement(2415..>3688)
                     /locus_tag="PL056_12210"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HPM47827.1"
                     /translation="VLPGAELCDSGHDENCDRTQMTAGGTGDPNEDGDAWTMCQGDCC
                     DNESQCLNPGIVNPGAVEFIGDSVDNDCNGSVDEARTGCLTGEKLNNTVIAMDLLKAM
                     DICQIHDPAKNNWGIVGTPSITRASGTGDVNYRQVGVMTRFGTSTTNNPIFGSNFAVL
                     SSGRARDAGDADATTTYTYEYANGNPPSDFVSAHGGSLPVTKTGCPAGSGANDSVMLK
                     VQLKVPTNANSFSFDFRFFSQEYWAFTCTEYNDFFITMLDTTWTPVNPGDKPIPADKN
                     ISFDSSGNYISVNSNQFFTVCDAKSGYTCPDGTGPLAGTGYNLKVCVEEDIWGNCEEE
                     KYNSGATKWLTTTAPVKPGETITLRFVIWDTSDMALDSLVLIDNFRWNAEPSGGAVTF
                     ACWDLNKNGTCDVASEDISGDNACDERDCKQ"
BASE COUNT         1030 a          796 c          665 g         1197 t
ORIGIN      
        1 aacctgaggc cctacctgag cagtatcacc gtctatcgcc tgacgacctg caagaactat
       61 gtctgctccg ccaagcattt ttattgcctc gctcaagaca taagaagttg caagagtatc
      121 agcaccggca tacgctctgt cagaaatgag ataaacctta tcggcaccca tgaaaagcga
      181 gtgtttcaga acatcaaccg ccttagccgg acccatcgat acaacagtca cctcacagtc
      241 accgagctta tcctttatcg aaagcgcaag ttcaagcgca ttaagatctt cgggattgaa
      301 aactgcggga agaacggctc tgtttaccgt tccgtcatct ttcattgcat tgcccgtaat
      361 gttcgcagta tcaggcacct gcttaactaa caccttgatt ttcaacatgt ttttcacctc
      421 cagacaaaaa tcaaactgac atgtcagtct agtaatatca gaaagtgttt tttaagtcaa
      481 tttttaagta atcttgattt atgaaacatt ggaataataa tttactgtaa ttatctcgtg
      541 ggggttactg tgtctgtatt aaaaaacaac agttcaaaat atcttgccgc atatctgttt
      601 tttctttgcg ttaccacagg aagattcttt tttgaggctc actacgctca ctttgtcgat
      661 actaagaatc tcatttttca tcaccactac tggtttctgt ttgtttttct cctttttctt
      721 attaatttta aattttttct aggcatgcac ccttccagat catggtggat cgcattttta
      781 agtcctgtga tatttattcc tctgatctat aacatgatct tcaggggagg aaatctcgta
      841 aagttcaact atctaagtgc gaaagatcct gattatttgc taaatatttt taccttcatg
      901 cttttttcag aaaagaacag accggttgga attgaactta ttatcgtcac tttctcaata
      961 tttctttttt catacttcat ttcaaaaaaa cctgtcagga gtgcaatttg tgccgtttcg
     1021 tgctatctct cgctcatgat ctttgcaggc accgtcatta tcgctcccca caaacccgat
     1081 ttcacactgt tttttattaa tagttcgttc aaacttcaga acttcatgtc ttttgtatat
     1141 ttttcagcat cagtcactgc cgctgtggta ctgtttttta atgaaatagt tgtttttttt
     1201 aaggatcaaa gacggcttat ctgttttgct gtaatattca catgtttttt catatgttct
     1261 caatttgcac tcatcaatcc ttcttcagcc gaccgtttct taatgatccc gcattctttt
     1321 cttttctcgt tatccattac agttctgatt tttatcaaca aggactttcc tctcaaaata
     1381 ctgctgtttt ttatatccat tatttcttca gggatacttt tccaactttt ctcaactttg
     1441 tggattcaga aaactcctga agaaacccaa ggcatgaact ttaatgacag aatagaacat
     1501 aaaatcgaag aaggaattga cattctttta tcaagacagg agcaaaatgg cgagttcaca
     1561 tctgtttcct gtaaatctcc ggatagaaaa caatgctctg tccataaggg ttccgtgttc
     1621 atttctactt ttattctata ctcgctcaat tttgtaaaaa gcaactcaaa agctcaggcg
     1681 atcgttaaaa aaggggcgaa ttttgtccag tccaaaatgg aagatggtga tctttggaga
     1741 ttttggggaa acagaataga ttacgacctt gatgacactt catgctcttc atttatactt
     1801 gagctcagtg acaaaagtct tttaaacaag gacaaaatat actctaacat ggaccagaaa
     1861 ggacttttta aaacatggat ccgtgaaagt ggaaaaaatg atgtagacgg tgttgtaaat
     1921 gcaaatgtac tgttgtatct tggagagaac agaaagacaa ttccggcatg cagcgcttta
     1981 gttgatgcgg taattgaaaa caatgaattt tccttgaatt attactaccc agatatcgct
     2041 tttttttatt acactctgtc cagagcgtat tttgaaaaac agatgaaatg cattgaaaag
     2101 ggaatccctg ttattgtcac aaaagttgaa aatatgctga atgaaagcat atctgataat
     2161 gatgtaatga gaactgctgt gcttctgaac actcttttaa attttaagga atattctaac
     2221 ctgatggatg tttctgcaaa aaaactgctt gagacaaatc ttcatgaaat atctcttgca
     2281 aaaaaacctt tttttgtcgc tggtgaacct cctgatgaac cttcattctt ttatttttca
     2341 aatgagactg taattgcatc attaattgag ttttttaaaa gatataacga cctgaaaaga
     2401 aattgagaaa acagttactg tttgcagtcc ctttcgtcac acgcattgtc accgcttata
     2461 tcttcgcttg cgacatcaca tgttccgttc ttgttgaggt cccagcacgc gaatgtaacg
     2521 gctccgccgc ttggctcagc attccacctg aagttgtcga tcagaacaag agaatcaagt
     2581 gccatatcag atgtatccca tattacaaat cgtagcgtta tggtttcacc cggttttaca
     2641 ggagccgtgg tcgtaagcca ttttgttgca ccggaattat acttctcctc ttcacagttg
     2701 ccccatatat cttcttcgac acagactttc aaattatatc cggtacctgc aagaggacct
     2761 gttccgtcag gacaggtgta tcccgatttt gcatcacaga ccgtaaagaa ctggtttgag
     2821 ttgacactta tatagttgcc tgaagaatca aaacttatat tcttatcagc gggaatcggt
     2881 ttatctcccg gatttacagg agtccatgtc gtgtcaagca tggtgatgaa gaaatcgttg
     2941 tattctgtac atgtaaatgc ccagtattcc tgagaaaaga atctgaagtc aaaagaaaaa
     3001 ctgtttgcat ttgtgggaac tttgagttga accttaagca ttactgaatc gttagctccg
     3061 cttcctgcag gacaaccggt ttttgttaca ggaagcgatc cgccatgggc tgaaacaaaa
     3121 tctgacggcg gatttccatt ggcatattca tatgtataag tcgttgtagc atcagcatcg
     3181 cctgcgtccc ttgctcttcc tgatgaaaga accgcaaaat tactgccgaa tatcgggtta
     3241 ttggttgtgc ttgtaccgaa acgggtcatt acgccgacct gtctataatt tacatcacca
     3301 gtcccgcttg ctctggttat cgaaggagtt ccgacaatac cccagttgtt ctttgccgga
     3361 tcgtgtatct ggcagatatc catcgcttta agaaggtcca ttgcgataac agtattatta
     3421 agtttttcac ctgtcagaca acctgttctt gcttcatcaa cacttccatt acaatcatta
     3481 tcaacactgt ctccgataaa ttcaacagca ccgggattga caattccggg atttaagcac
     3541 tgcgattcgt tgtcacaaca gtcaccctga cacattgtcc atgcatcacc atcttcattg
     3601 ggatcacctg ttccgcctgc tgtcatctgc gttctgtcac agttttcatc atgtccgctg
     3661 tcacaaagct ctgcaccggg aagcacca
//
LOCUS       DAOOYB010000284         3647 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_30332_length_3647_cov_11.908129, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000284 DAOOYB010000000
VERSION     DAOOYB010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3647
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30332_length_3647_cov_11.908129"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            173..1186
                     /gene="ruvB"
                     /locus_tag="PL056_12215"
     CDS             173..1186
                     /gene="ruvB"
                     /locus_tag="PL056_12215"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008400776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HPM47828.1"
                     /translation="MEDRLVGSEIKFEDSSLEGGLRPQNLSGYIGQKTVVDNIKIYIE
                     AAKRNDRQLDHLLLAGPPGLGKTTLSQIVANEMGVKIFITSGPAIEKKGDLAGLLTSL
                     KEKDVLFIDEIHRLNASVEESLYSAMEDFFFDIMLGEGAHAKSMRLPIEPFTLIGATT
                     KTGSLSSPLRDRFQIVFRMDYYSVEELKKIVERSSEILGIKTSSEGALEIARRARGTP
                     RIANRILKRIRDFAEIKGDGTIGLDITRYALDQMEIDEDGLDQMDRTILLTIIESFNC
                     GPVGVESIAASVSEEKRTLEEVYEPYLIKSGFIARTPKGRVVTKKTLEKFGIENTFQG
                     KLF"
     gene            1183..2412
                     /locus_tag="PL056_12220"
     CDS             1183..2412
                     /locus_tag="PL056_12220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47829.1"
                     /translation="MKKYYQQLKKIHDFHSLERNDLKDYIHLQKTEVSNLLSIPPEFW
                     VIIDYVIHLTRAVLKKPKIEAEKDLKSVIKPLFDCYNSKIERILRKMPENEFLYLKQD
                     MKSFHHIICHSVNLIDKGLPHFIPSYLFIDSIEKMTVLYPLHENCCDHGTLARGNYPV
                     LMSRYISESFTPGIRDIINFLIWLQNILPHAITDDRVYLRNRMISIMEEFISNEHQFK
                     NAEDYSTKELVIELREVITVSYIRENIISLLEKIKSPASQIPVSECLDHIASIFIETG
                     KLSPERTTARDFAIATEMCGKIIEGIKNESEKKVLYENLVLANALKPYFLKVTRYMLD
                     PKTDLELCRLIEICDFLFVTYPSVRATDELLELLENIHKSLETERELVCSTNSVTIDP
                     EAMNLFKSKMKQVSARN"
     gene            complement(2409..3482)
                     /locus_tag="PL056_12225"
     CDS             complement(2409..3482)
                     /locus_tag="PL056_12225"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HPM47830.1"
                     /translation="MSTSTRILFVIFGIPLIFFSFFIGASFYFEYRPEKVENALKIRD
                     GGAVFKEGKIKILSWNIGYAGLGKSSDFFLDGGNSSKPENIETVRKNLDEIKKKIKEI
                     NADIIFLQEVDVESSRTFNILQAFEIANENKEYGSWFATNFRTFSVPVPWYSPMGKIW
                     SGIMTLSKFRFTDEVTRHSLPENLKLPERLFDLKRCMLVSRILLDEEGRELVLVNLHL
                     SAFDDGELKKQQIEFVVEFLVNEHKKGNMVIAGGDWNHSMPGIGKEHFGSYTTKEENL
                     KWLGRIDEKLAPAGWSWVFDKDNPSVRSNEKGYVKGENFTTVIDGFLVSPGITVKEIR
                     TVVSQFEHSDHEPVVVEINIENN"
BASE COUNT         1149 a          786 c          694 g         1018 t
ORIGIN      
        1 cggtcattac tgattcagac agaaacactc tcgatgcaca ggaagggctt cttgcccttg
       61 gatacaatcc tcttcaggta agacatgcgc ttgccaaaat ttctgaaaaa gataagatga
      121 aagctgaagc gatagttcga gaggcgttgc ggataattaa cggtaagaga taatggaaga
      181 ccggctggtt gggtcagaaa tcaaatttga agattcatcc cttgaagggg gtcttcgtcc
      241 tcagaacctt tccggctaca ttggccagaa aacagtcgta gacaacataa aaatatatat
      301 cgaggcggca aaaagaaatg accgccagct tgaccatctt ctcctcgcgg gacctccggg
      361 gcttggcaag acaacattaa gccagatcgt ggcaaatgag atgggtgtga agatattcat
      421 aacaagcggg cctgccattg aaaagaaagg tgaccttgcc ggacttctga catccttaaa
      481 ggaaaaagat gttcttttca ttgatgagat tcaccggctc aacgcctctg tggaagaaag
      541 tctttattcc gcaatggaag attttttctt tgacataatg cttggcgaag gggcacatgc
      601 caaaagcatg cgacttccca ttgaaccgtt cacacttatt ggagctacaa caaagactgg
      661 atcactttca agtcctctcc gtgacagatt tcagatcgtt ttcaggatgg attattattc
      721 agttgaagaa ctcaagaaaa tagttgagcg gtcatctgaa atactcggca taaaaacaag
      781 ctctgaaggt gctcttgaga tcgcaagaag agcacgcgga actccgagaa tcgcaaacag
      841 gatacttaaa aggatcaggg atttcgccga gatcaaaggt gacggaacaa taggacttga
      901 catcacaaga tacgctcttg accagatgga aatagacgaa gacggacttg accagatgga
      961 tagaacgatc cttcttacta taatcgaatc tttcaactgc ggacccgtcg gagttgaatc
     1021 tatcgccgca tcagtaagcg aggaaaagag aactcttgag gaggtttatg aaccttatct
     1081 cattaaatcg ggtttcattg caagaacacc gaaagggcgg gtcgttacaa agaaaactct
     1141 tgaaaaattt ggaattgaga atacttttca ggggaaactg ttttgaaaaa atattaccaa
     1201 cagctcaaaa agatccatga ttttcactct cttgaaagaa acgacctgaa agattacatc
     1261 catcttcaga aaacagaggt ttccaatctt ctttcaattc cgcctgaatt ctgggtaata
     1321 attgattatg tgatacacct gacaagagca gttctgaaaa aaccgaaaat tgaagccgag
     1381 aaagatttga aatcagtcat aaagccgctt tttgactgct ataattccaa gattgaaaga
     1441 attcttagaa aaatgcctga aaatgaattt ctgtacctga aacaggatat gaaaagtttt
     1501 catcatatca tctgtcactc ggtaaacctt attgacaaag gacttcccca cttcattccg
     1561 agctatcttt tcattgattc gattgaaaaa atgactgttc tgtatcctct tcacgagaac
     1621 tgctgtgatc acggaacact tgcaagaggc aattaccctg ttctcatgtc aagatatatt
     1681 tcggaaagtt tcaccccggg catcagggat atcatcaatt tccttatctg gctgcagaac
     1741 atcctgcctc atgccataac cgacgacaga gtttatctca gaaatagaat gatcagcatt
     1801 atggaagagt tcatttcaaa cgagcaccag ttcaaaaacg ccgaagatta ttcaaccaaa
     1861 gagcttgtca ttgaactccg cgaagtaata acagtttcat atatcagaga aaacatcatc
     1921 tcactccttg aaaaaataaa atcgcccgct tcacagatac cggtgtctga atgtcttgat
     1981 cacattgcat caatattcat agaaaccggc aaactttcac ctgaaagaac aacagcgaga
     2041 gatttcgcaa ttgcaaccga aatgtgcggg aagatcattg agggaataaa gaacgaatct
     2101 gaaaagaaag tcctttacga aaaccttgtc cttgcaaatg cgttgaagcc gtattttctt
     2161 aaagtcacca gatacatgct cgatccgaaa acagatctgg agctgtgcag acttatagaa
     2221 atatgcgatt ttctctttgt cacataccct tctgtcagag caactgatga actgctggaa
     2281 cttcttgaga acattcataa atcacttgaa actgagcgtg agcttgtgtg ctctactaat
     2341 tcagtgacaa ttgatcccga agcaatgaac cttttcaagt cgaagatgaa gcaggtcagt
     2401 gcaagaaatt aattattttc aatatttatc tcaacaacaa ccggttcatg atctgaatgt
     2461 tcaaactgtg aaacgactgt cctgatctct ttgaccgtga tgccgggact gacaaggaat
     2521 ccatcaatga ccgtggtaaa gttctctcct ttaacatatc ctttctcgtt tgaacggacc
     2581 gatggattat ctttgtcaaa aacccagctc caccctgccg gagcaagctt ttcatcaata
     2641 cgccccagcc attttaggtt ttcctctttt gttgtgtatg agccgaaatg ttctttcccg
     2701 atacccggca ttgaatggtt ccagtcacct ccggcaatta ccatatttcc ttttttgtgt
     2761 tcattaacaa gaaattcaac aacaaattcg atctgttgtt ttttcaactc accatcatca
     2821 aacgcgctca gatgaaggtt gaccagcacc agctctctcc cttcttcatc aagcaatatt
     2881 cttgaaacga gcatgcatct tttaaggtca aacaaccttt ccggcagttt taagttttcc
     2941 ggaagagaat gcctcgtcac ttcatcagta aatctgaact ttgacaaagt cataatgcct
     3001 gaccagattt tccccattgg tgaataccaa ggaaccggaa cagaaaatgt cctgaaattt
     3061 gtcgcgaacc agcttccata ttctttattt tcattagcaa tttcaaatgc ctgcaaaata
     3121 ttgaatgtcc ttgaagattc aacatcaact tcctgaagaa atatgatgtc ggcattgatc
     3181 tcttttattt ttttcttaat ttcatcaagg ttcttccgaa ccgtttcaat gttttccggc
     3241 tttgaactat tcccgccgtc aagaaaaaag tcggaagatt ttccaagacc tgcataaccg
     3301 atattccagc ttaatatttt tatctttccc tctttaaaaa ccgctccgcc atcacggatc
     3361 ttcaaagcat tttcaacctt ttcaggcctg tactcaaaat agaacgaagc cccgataaaa
     3421 aatgaaaaaa atatcagcgg aattccgaag atcacaaaaa gtattctggt tgatgtgctc
     3481 atctctcact tcaaaaaaaa gtttactcaa aagagtttat attatttttt aaaaaatgga
     3541 aaaaatgttg attgaaaatc tactctttcg ttttgatctc ttttggagtt aatgaatctt
     3601 cgtatgtgcc gttgccaagc tgtccgcttg tattcaatcc ccagcag
//
LOCUS       DAOOYB010000285         3632 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_30496_length_3632_cov_11.336874, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000285 DAOOYB010000000
VERSION     DAOOYB010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30496_length_3632_cov_11.336874"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..3380
                     /locus_tag="PL056_12230"
     CDS             78..3380
                     /locus_tag="PL056_12230"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HPM47831.1"
                     /translation="MRYIGIDVGSTTVKAVVVNENNEIIWKKYSRHNTRQPELVRDFL
                     NEIKDLFVKEDLAVFITGSGSRAIASAINAQYIQEVNAVCFAVETMHPDVSSVIELGG
                     QDAKVIIWQGEVDDRSTLTFMNDKCAGGTGATIDKILDKIGLSTVEANKIRAHGKTIN
                     HIAAKCGVFAETDVVGLLKAGVDKDDIFVSLCVAIVKQNLEVLVRGNVLKDKVLLLGG
                     PHTYLPALTDIWRLQLPETWETHKYSPTEPDLEKAIFVPEDSQYFAALGAILFGRRSA
                     ELSKIADIPVFKDTEKLEEYISQGRLAKLESSGAIVEGLIQNDEEIEEFAKIYSIPEF
                     VPPKFEKVKTYECYLGIDGGSTSTKLVLLNKNKELIYKDYLLSNGNPVNDVKNMFSRM
                     IKWENEQEIGLKIIGSAVTGYASAILKTAFNLDRSIVETVAHMKSATATYGDIDLICD
                     VGGQDIKVLFMKHGRVVDFKLNTQCSAGNGFFLQGMAAQFNIPVEEYAIHAFKARKAP
                     AFNYGCAVFMEQDKVNFQQLGWTKEEIMAGLALVLPKNIWNYVVQESNIARFGKRFVL
                     QGGTQKNLAAVKAQVDYIRSKIPDAQVFVHKYSDVCGAIGAAMEAVESVHDNSSFIGI
                     ENGAKIEFTTKNNESTRCRICANRCPRAFIDIARPDGANIRFISGYQCEKGMADSVEE
                     MKEFAKTRADALKKTPNLVDMAAHLVFSDFEFDPLPKNGADVNQSSYYPENELNPKNG
                     LKFAGSSTEVLEKRQKMVVGIPKLLNMYLYTPFFSTYFRTLGMKVVFSSFTSDDLWAK
                     GNKWGAIDPCFPAKVAPAHIFELISKKECTHICFPIITHLHSSVKNTLGNNACVIQMG
                     TPEVVNAVFKRETDFFADNNIDYWRPLVSMDNPVEINEILFEYFKEKFELTADENAWA
                     VHQGLKAMGTYYETLQNEGARIINDLILQDKMGVLLVGHPYHHDPGLNHKILEKFQLK
                     GFPVLCIESLPDSDSFIEPLFENSNKEKSFDITDVWWRNFNRNTNLKVMAARVAARHP
                     NLAVIDLSSFKCGHDAPTYSYIDNILDASATPHFLFHDIDQNKPGATFNIRIETIEYF
                     LKHEEEMLKGKG"
BASE COUNT         1096 a          685 c          844 g         1007 t
ORIGIN      
        1 atttctaagg atattgactc tatctttttt aagtgataaa aactgcagtt gattttttga
       61 atatatccgg gggtcggatg aggtatatcg gaatagatgt cggttcaacg acagtaaaag
      121 cggtagttgt caatgaaaat aacgagataa tctggaaaaa gtacagtcgg cataacacga
      181 gacagccgga gttggtgagg gattttctga atgagataaa agacctgttt gttaaagaag
      241 atctagcggt gtttattacc ggcagtggtt caagagcaat cgcttcagcg ataaatgctc
      301 aatatattca agaggttaat gcggtgtgtt ttgccgttga gacaatgcat ccagatgttt
      361 cctcagtcat tgaactcggt ggacaggatg caaaagttat catctggcag ggtgaagttg
      421 acgaccgttc gacattaact ttcatgaacg acaaatgtgc cggcggaact ggtgctacaa
      481 ttgataaaat tcttgataag atcggtcttt caacagttga agccaacaag atcagagctc
      541 acggaaagac gataaatcac attgccgcca aatgcggagt ttttgctgaa accgatgttg
      601 tggggcttct gaaggcaggc gttgacaagg atgatatttt tgtttctctg tgtgttgcta
      661 tcgttaagca gaacctcgaa gttcttgtgc ggggcaatgt ccttaaagac aaagttcttc
      721 ttcttggcgg gccgcacaca tatcttcctg ctcttactga tatatggcgg cttcagcttc
      781 ctgaaacctg ggaaacgcat aaatattctc caacggaacc cgatcttgaa aaagcgatat
      841 ttgttcctga agattcgcag tattttgcag ctcttggagc aatacttttc ggaagaagat
      901 cggctgaact ttcaaaaatc gcagatattc cggtttttaa agatactgag aaacttgaag
      961 aatatatttc tcagggccgg cttgctaaac ttgaatcaag tggagctatc gttgaggggt
     1021 taattcaaaa tgatgaggaa attgaagagt ttgcaaaaat ttattctatc cctgaatttg
     1081 tgccgcccaa gtttgagaaa gtgaaaactt atgagtgtta tctgggaatt gacggcggtt
     1141 cgacaagcac aaaacttgtt ctgttaaaca aaaacaaaga acttatctac aaagattatc
     1201 tcctttcaaa cggcaatccg gtaaacgatg tgaaaaacat gttctcacgg atgataaaat
     1261 gggaaaatga gcaggaaata gggcttaaga tcatcggaag tgctgtcacc ggttatgctt
     1321 ctgcgatttt gaaaactgct ttcaatcttg acagatctat cgttgaaact gtcgcccaca
     1381 tgaagtctgc aacggcaact tacggtgata ttgatcttat ctgcgatgtc ggaggtcagg
     1441 acataaaagt tcttttcatg aagcacggaa gggtcgttga tttcaagctc aatactcagt
     1501 gcagtgcagg gaatggcttt tttcttcagg ggatggcggc tcagtttaac attccggttg
     1561 aagaatatgc aattcacgct tttaaggctc ggaaagctcc tgcgtttaac tacggatgtg
     1621 cagttttcat ggaacaggat aaagtcaatt tccagcagct tggctggaca aaggaagaga
     1681 tcatggcggg acttgcactg gtgcttccga aaaacatctg gaactatgtc gttcaggaat
     1741 caaacattgc acggtttggc aaaaggttcg tccttcaggg gggaacacag aaaaatcttg
     1801 ccgcagttaa agcgcaggtt gattatatca gatcaaaaat tcctgatgcg caagtgttcg
     1861 ttcataaata ttcagatgtc tgcggagcga taggtgcggc aatggaagca gttgaatctg
     1921 ttcatgataa ttcatctttc ataggcattg agaatggtgc aaaaattgag tttacaacaa
     1981 aaaacaacga atcaaccaga tgccggatat gtgccaacag atgtccgaga gcttttattg
     2041 atatagcgag accggatggc gcaaatataa ggttcatttc gggttatcag tgtgaaaaag
     2101 ggatggctga cagtgttgaa gagatgaaag agttcgcaaa gacaagagca gatgcactta
     2161 agaaaactcc caatcttgtt gatatggcgg ctcatcttgt tttcagtgat tttgagtttg
     2221 atccacttcc caaaaacggt gcagatgtga accagtcgag ctattatccg gaaaatgaac
     2281 ttaaccccaa aaacgggttg aaatttgccg gttcatcaac ggaagtgctt gaaaaaagac
     2341 agaaaatggt tgtaggaatt ccgaaacttc ttaacatgta tctctatact ccgttctttt
     2401 cgacctattt cagaacgctt ggaatgaaag tggttttcag cagtttcacc agtgatgatc
     2461 tctgggcgaa agggaataag tggggggcga ttgacccatg tttcccggcg aaggttgctc
     2521 cagctcatat atttgaatta attagcaaaa aagagtgtac gcacatctgt tttccgatca
     2581 tcacccatct ccattcttcg gttaaaaaca ctcttggcaa caatgcctgt gtaattcaga
     2641 tggggactcc tgaagttgtg aatgccgttt ttaaaagaga gaccgatttt tttgccgata
     2701 acaatattga ttactggcga ccactggtga gcatggacaa tcctgttgag ataaatgaga
     2761 tcctttttga atatttcaag gaaaagtttg agcttactgc tgatgagaac gcatgggctg
     2821 tgcatcaggg gcttaaagcg atgggaacat attacgaaac acttcagaac gaaggggcca
     2881 gaataattaa tgacttgatc ttacaggaca aaatgggagt tcttctcgtt ggtcatccgt
     2941 atcatcatga ccccggactt aatcacaaga tccttgaaaa atttcagttg aaaggatttc
     3001 cagtgctctg cattgagtca cttcccgata gcgacagttt catagagccc ctttttgaaa
     3061 attcaaacaa agaaaaaagc tttgacataa ccgatgtctg gtggagaaac ttcaatagaa
     3121 acaccaactt aaaagttatg gcggcaagag ttgcggcaag acacccaaac ctcgcagtaa
     3181 tcgacctcag cagctttaaa tgcggacacg acgcacccac ctattcatat atcgacaaca
     3241 tccttgacgc atcagccaca ccgcattttc tcttccatga catagaccag aacaaacccg
     3301 gagccacctt caacatccgc atcgaaacca tcgaatattt cctcaaacat gaagaggaaa
     3361 tgctgaaggg gaaggggtag atttgtgcaa cagcttgttg gacaaatcag aaaaagcgaa
     3421 aataatttag tgatttgaga aggatcttat gcaaaagaca ttaggtagaa atggggtttt
     3481 tacccttatg gctgctccga aagccctgaa agtttttgtg cgatcttttc cggtgtaggt
     3541 agaagacttt tcattgattc aggcaatgtt tttgtggttt tataagtggc tccatccgat
     3601 ttctcgcact aatggtgcga gtttgtcatt tt
//
LOCUS       DAOOYB010000286         3600 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_30901_length_3600_cov_16.313681, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000286 DAOOYB010000000
VERSION     DAOOYB010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3600
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30901_length_3600_cov_16.313681"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="PL056_12235"
     CDS             <1..492
                     /locus_tag="PL056_12235"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HPM47832.1"
                     /translation="FDEDVWIEEVVKMLKQNKLGSARMRMILDGTGVPKISFEPFADD
                     LSSVLVRFKEISPQGNIKIWPFRKHTEKSDEEVILIDKNTGNILEGSYTNVFVKIGDE
                     FFTPPDDERILPGICRKHFIRYLKLKGMIVREEYFGIEKLKNSEIFLTNALRGVVEGI
                     YIP"
     gene            complement(480..839)
                     /locus_tag="PL056_12240"
     CDS             complement(480..839)
                     /locus_tag="PL056_12240"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPM47833.1"
                     /translation="MKIGFVTIHVSDLDKTISFYENVMGFSIKRRFKAGPQVEIVFMD
                     DGSGNQLEFITGTGHFVEAKGISIGFNVEDIEKTHDHLKNHGVRIVFGPATMPSGVKL
                     LHAVDLNGVELGFVQGM"
     gene            963..>2138
                     /locus_tag="PL056_12245"
     CDS             963..>2138
                     /locus_tag="PL056_12245"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HPM47834.1"
                     /translation="MIEFSSALKIVMDHVPKYDKKETVDINDCINRVLAFDISADRPA
                     PPFNRVAMDGYACRKNDIGNELKIVEIVPAGKTAEKFINPGECSKVMTGCPLPDGAEM
                     VFMIEVAVEKNGLVTCTDPEIAKKHENYSKTGEDAKKGDIVIRKGKVIEPRHIPAIAT
                     NGYSKLVVMKRPVVGIIATGDELVEPFETPLPHQIRNSNSYQLSAQVIRAGGVPKYYG
                     IVKDDPEKTEKIVKTAKSECDIILMSGGVSAGDFDFVADSLVKAGFQIIFDSVAVKPG
                     KPTTFAVSDEAICFGMPGNPVSTFVIFEIIVRPFIMKMMNADHKPAHVEMILQNGLKR
                     KRVERKEYVPVKILDSRTVFKPEYHGSGHLTSLADADGIIVIEKGEHEIASGSKVEVL
                     "
     assembly_gap    2139..2238
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2239..>3600)
                     /locus_tag="PL056_12250"
     CDS             complement(<2239..>3600)
                     /locus_tag="PL056_12250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47835.1"
                     /translation="FFFSACDIEKRSVAIDESVQDEDVEIMNDEEMVDDEQVLEFVEI
                     TMQKVSGNLSQKAVVDVEEFKVERNLNHVDTSFFIHMNDLEIIAKKLRNEILEKGCHD
                     CSEDENYLFAVTSKNDECCVYDEQVINEIAKYLLIPVNSMLWPFAPQDEPHNVGHTSV
                     NIQHYTNKYEEAYFHHGVDIIKNEPEKIFNMYDGRVEKIGYYRTEEVGESKYYFEVVV
                     QTVNGLKFEFHHTDPESVPDAVYELEGTDKVLKAGEETGKIVFWPTPDSFSGKLFHHI
                     HFNVITESGIKLNPLIMMTPQKDDTAPEIEEIYLIDPDRTKTLSTENISENFHVVIKV
                     SDLADNEIWPNPPRYSMVEIKNSDDETIFEHSGYDFIAMMSENINEFVCDYYLCEIGE
                     TVFSKGNYSQREFHVVVTAFDKTGNITDAIEKELFENGDYSIKATSCDEVGNCSEKSI
                     DISF"
BASE COUNT         1094 a          717 c          660 g         1029 t
ORIGIN      
        1 tttgatgaag atgtgtggat tgaagaagtt gttaaaatgc ttaaacagaa caagcttggc
       61 agcgcaagga tgaggatgat actggatgga acgggtgttc caaagataag ttttgagccg
      121 tttgctgatg acttgagctc tgttttggtc agatttaaag agatctcacc gcagggaaat
      181 ataaaaatat ggccgtttag aaaacatact gaaaaaagtg atgaagaagt gatcctgatc
      241 gacaaaaaca ctggaaatat ccttgaagga agttacacca atgtttttgt taaaattggt
      301 gacgaattct tcactccgcc cgatgacgag agaattcttc cggggatctg cagaaaacat
      361 tttatccgct acctgaaatt aaaaggaatg atcgtccgcg aggaatattt tggaattgaa
      421 aaactcaaaa actctgaaat atttctcaca aatgctttgc ggggagttgt tgaagggatc
      481 tacatcccct gaacaaaacc gagttcgact ccgtttagat caactgcgtg aagaagtttc
      541 actccgcttg gcattgtcgc cggaccgaaa actattctga caccgtggtt tttaagatga
      601 tcatgtgttt tttcaatatc ttcaacatta aacccgatcg agattccctt cgcttcaacg
      661 aaatgacccg ttcctgtgat gaattcaagc tgattaccgc ttccatcatc cataaaaact
      721 atctcaacct gaggccccgc cttgaaccgt cttttaatcg aaaatcccat gacattctca
      781 taaaacgaaa tcgtcttatc aaggtctgac acatgaattg taacaaatcc gatcttcatt
      841 gcttgctcca taaaaaatta atcagaaaac aaccaaagaa ttataacaca ctgcacaaaa
      901 acatcaactt gcctttagta actgttgtgt taatatcatt tgaatttttt gcggagaaaa
      961 aaatgatcga attcagttca gcattaaaaa tcgttatgga tcatgttcca aagtacgata
     1021 aaaaggaaac ggttgacatt aacgactgta taaatcgtgt tcttgcgttc gatatttcag
     1081 cagacagacc ggctccgcct ttcaaccgtg ttgcgatgga tggatatgcc tgtaggaaaa
     1141 atgatatcgg caatgaactc aaaattgttg agatcgttcc tgcaggaaaa acggctgaaa
     1201 agttcatcaa tccgggtgaa tgttctaaag ttatgaccgg atgtcctctt cctgacggag
     1261 ccgaaatggt gttcatgatc gaagtcgctg ttgaaaagaa cggacttgtt acatgcacag
     1321 atccggaaat tgcaaaaaaa catgagaatt attcaaaaac aggcgaagat gcaaaaaaag
     1381 gtgatatcgt aatcagaaaa ggaaaagtga ttgagcccag acacattccg gcaattgcca
     1441 caaacggata ttcaaagctc gttgtgatga aaagacctgt cgtcggcatt attgcaacag
     1501 gagatgagct ggtcgaaccg tttgaaacac ctcttcccca ccagataaga aattctaaca
     1561 gctaccagtt gtccgctcag gtaataagag caggcggagt tccaaaatat tacggcatag
     1621 taaaagatga tccggaaaaa actgaaaaga tcgttaaaac cgccaaaagc gaatgcgaca
     1681 taattctcat gagcggcgga gtaagtgccg gcgactttga ttttgtagct gattcccttg
     1741 tcaaagcagg gtttcagatc atttttgatt ccgttgcagt aaaacccgga aaaccgacga
     1801 cttttgccgt ttcagacgaa gcgatctgtt tcggaatgcc gggaaacccc gtttcaacct
     1861 ttgttatctt tgaaattata gtcagaccct ttattatgaa aatgatgaat gccgatcaca
     1921 aaccggctca tgttgaaatg atccttcaga acggtcttaa aagaaaaaga gttgaaagaa
     1981 aagaatatgt gccggttaaa attttagaca gcagaactgt ctttaaaccc gaatatcacg
     2041 gttccggtca tcttacctct cttgccgacg cagacggcat tatagtgata gaaaagggag
     2101 agcatgagat agcatccggt tcaaaagtgg aagttcttnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnga aacttatatc aattgacttt tcgctgcagt tcccgacttc
     2281 atcgcagctt gttgctttta ttgaatagtc accgttctca aaaagctctt tttctatggc
     2341 atcagttatg ttccctgttt tatcaaaagc cgtcacaaca acatggaact ccctttgaga
     2401 ataattccct tttgaaaaaa ccgtttctcc tatttcgcaa agatagtaat cacaaacaaa
     2461 ctcatttata ttttcactca tcattgcgat gaaatcatat ccggaatgtt caaaaatagt
     2521 ttcatcatca ctgttcttta tttcaaccat tgaatagcgg ggcggattag gccagatctc
     2581 attatcggca agatcagaaa cttttatgac cacatgaaaa ttttcgctaa tattttcagt
     2641 tgaaagagtt tttgtcctgt caggatcgat cagatatatc tcttctattt caggagcagt
     2701 gtcatctttc tgaggagtca tcattatcag cggattgagt tttattccac tttctgtaat
     2761 gacattgaaa tgaatgtgat ggaaaagctt tccgctgaaa gaatcaggtg tcggccagaa
     2821 aacgattttg cctgtttctt cgcctgcttt caaaacttta tcagtccctt ccagttcata
     2881 tacggcatcc ggaacacttt cggggtctgt gtgatgaaac tcaaatttca atccgttcac
     2941 cgtctgcacg acaacttcaa aataatattt gctctctccc acttcctctg tcctgtaata
     3001 accaatcttt tcaactcttc cgtcatacat gttaaagatc ttttccggct cattttttat
     3061 gatgtcaact ccgtgatgga aataggcctc ttcatattta ttagtgtagt gctgaatgtt
     3121 gacacttgtg tgaccgacat tatgcggctc atcctgtgga gcaaaaggcc agagcataga
     3181 atttaccgga ataaggagat attttgcaat ttcatttatc acctgttcat cataaacaca
     3241 gcactcatca ttctttgaag taaccgcaaa aagataattt tcgtcttcag aacaatcatg
     3301 acatcctttt tctaagatct cgtttctaag cttttttgca ataatttcaa ggtcgttcat
     3361 gtgaatgaaa aaagaggtgt caacatggtt taaattcctt tcaactttaa actcttcaac
     3421 atcaacaact gctttctgac tcagatttcc tgacactttc tgcattgtta tttcaacaaa
     3481 ttcaagcacc tgttcatcat caaccatttc ttcatcattc attatctcaa catcctcatc
     3541 ctgaacagat tcatcaatgg caacagatct tttttcgata tcacaagctg aaaaaaagaa
//
LOCUS       DAOOYB010000287         3598 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_30924_length_3598_cov_8.399379, whole genome shotgun sequence.
ACCESSION   DAOOYB010000287 DAOOYB010000000
VERSION     DAOOYB010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30924_length_3598_cov_8.399379"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(166..1149)
                     /gene="trpS"
                     /locus_tag="PL056_12255"
     CDS             complement(166..1149)
                     /gene="trpS"
                     /locus_tag="PL056_12255"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018449646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HPM47836.1"
                     /translation="MRALSGIKPTGTLHIGNYFGAAKQFEMMQEKNYEGYYFIADYHT
                     LNGYPEPAKLTDHTWNIVLDYLAFGLDPQKSVIFLQSQVPEVVELAFILGNYTPMGLL
                     QRAHSYKDKTAKNEQINVGLFYYPLLMSADILLYKSNVVPVGKDQKQHVEIARDIAEK
                     FNLHYQKDVFVLPEPYIPEDVAIVPGTDGQKMSKSYQNTIEMFADDATLKKQIMGILS
                     DSTPLEAPKDPAKCHIYNIFKLFVTKERSNEMAEKLLAGGYGYGHAKKELLEAVHTFF
                     DPMRKKRAELADNMDFVEKVLKEGSEKARTVAIETIKEVKKVTGLVGNIYN"
     gene            complement(1164..3437)
                     /locus_tag="PL056_12260"
     CDS             complement(1164..3437)
                     /locus_tag="PL056_12260"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HPM47837.1"
                     /translation="MRGIRKFFISHWFVLIPFFFYYFTMATKMALGDVALMVHRMVIL
                     ELGIHPNLHNLAMLVGNLFLKLPFGTVIYRANLFTVVFGAASVISFYFLLNKLGLRKF
                     VTVASSFIFMVSHSVWWHATMAENYILHVFMIIVILICLVDYTQRKVIWPLYLSFFLA
                     GLSFFNHVQNGVWWGGCYLFVLFSFSDIQKKGHLPYFYRKIFKEKRSFLFYWIFIVSS
                     FYLLLGVLPYLILLFRTWAAKGYDLAKTFNHGAGGEFKNIMFDFDNMSGFYQSFWYHL
                     LQFPTPFYFLTVLGLLYFVSGWFISRTVKPSENRSMVEKAVLSTLLTLSILTALTFGI
                     LFHNGAVFEIFGTKLTWFRYDSGIRMFVANVFIALFLMLILQRKGLLKDPFKGEYWKV
                     FYSALILPFVVTLVFFQFYNTWDQFQFLLPCFIIMALFASVMFNQIVEIIENNKRKSI
                     LYSVYAISVLSVIFPVWYYQKISDWSVDPSSWWFDFGPYSDQRFLNSHNRSEYNNNPD
                     KSGYDDVDEFVNLLFAKLPQNATILDDDSRMFYPIHLYYRKYADRDDKGRPDINLKMI
                     NVWGHKNWGTTVDRVVREVNAVNPGEENYFLIANRNYPHYDVIKKLDHNDRTFLPWKL
                     RNNRWIYKLKVFTPEEKNRLGSFPPLSFNYMYFGADFDTNGLNIKEKFKADEKIEARV
                     TFGRLPKNIEPFDMVFTLSDTSGNRVSEKKMVIKGGWRGIQVPLETEKLNLKTGNYEV
                     VVTVKEIEIFRRSFEIY"
     gene            complement(3450..>3598)
                     /locus_tag="PL056_12265"
     CDS             complement(3450..>3598)
                     /locus_tag="PL056_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010232053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HPM47838.1"
                     /translation="GEGLGLDSIDALSLIVLLEREYGKKIKDTETGKKVLYSIKTMAE
                     FIKS"
BASE COUNT         1100 a          732 c          712 g         1054 t
ORIGIN      
        1 cctgcagaga tgagaaaaac agtttttacg ccggtagaga cattattctc ctttgaaagg
       61 atgagcgagc cgtaaacagg ggcacatgtc acaagacagg tggttccgag tgtaagtccg
      121 aggagaaaac cttcagttag aaattgtatc aatgaaaatc cttaattaat tatagatatt
      181 gccgacgagc ccggtcactt ttttaacttc ttttatcgtt tcgattgcga ctgtgcgggc
      241 tttttcgctt ccttctttga gcactttttc aacgaagtcc atattatcgg caagttccgc
      301 tctctttttt ctcatcggat caaaaaatgt atgcactgct tcaagaagtt ctttctttgc
      361 atgtccgtaa ccatatccgc cggcaagaag tttttcagcc atttcatttg atctttcttt
      421 agtgacaaac agcttgaata tgttataaat atggcatttt gcaggatctt tcggtgcttc
      481 aaggggagtt gaatcggata atattcccat gatctgtttt ttcaaagttg catcatcagc
      541 gaacatttcg atggtgttct gataactttt tgacattttc tgtccgtcag tccccggaac
      601 gatcgcaaca tcttcaggaa tgtaaggttc aggaagaaca aagacatctt tctgataatg
      661 aagattgaac ttttcagcaa tgtcacgggc aatctcgaca tgctgtttct ggtctttgcc
      721 gacaggaacg acatttgatt tgtaaagaag aatatcagcg ctcatcaaaa gcggataata
      781 gaaaagtcct acattgatct gctcattttt tgcagtttta tccttgtagc tgtgtgctct
      841 ctgcagaagt cccatcggag tgtagtttcc cagaatgaaa gcaagctcaa caacttccgg
      901 aacctgcgac tggagaaata tcacactttt ttggggatca agtccgaaag caagataatc
      961 aagaacgata ttccaagtgt gatcagtaag tttcgccggc tcaggatatc cgttcaaagt
     1021 gtggtagtct gcaatgaaat aatagccctc gtaattcttt tcctgcatca tttcaaactg
     1081 ttttgccgca ccgaaatagt tgccgatatg gagtgttcca gtcggtttga tacctgataa
     1141 agctctcatt ttaacccctt gttttagtat atctcaaatg atcttctgaa tatttcgatc
     1201 tcttttactg tgacgacaac ttcatagttt cctgttttca ggttgagttt ttcagtttca
     1261 agtggaacct gtatccctct ccatccgcct tttatcacca ttttcttttc tgaaactctg
     1321 ttcccggaag tgtcagaaag ggtaaacacc atgtcaaatg gctcaatgtt tttcggaagc
     1381 cggccgaaag tgactcttgc ttctattttt tcatctgctt tgaatttttc tttaatattt
     1441 aacccgtttg tgtcaaagtc agcaccgaaa tacatataat tgaaactcaa gggaggaaat
     1501 gatcccaacc ggtttttttc ctctggggtg aatactttca atttgtatat ccacctgtta
     1561 ttacgcagtt tccacggcag gaaggttctg tcgttgtgat caagtttctt tatcacatca
     1621 taatgaggat agttcctgtt tgcgataaga aaatagttct cttcaccggg attgacggca
     1681 ttaacttcac ggacaactct gtcaacagtc gttccccagt tcttatgacc ccacacattt
     1741 atcatcttga gatttatgtc aggccttcct ttatcgtctc tatcggcata ttttctgtaa
     1801 taaagatgta tcggatagaa cattctggaa tcatcatcaa gtatcgttgc attttgagga
     1861 agtttcgcaa aaagaaggtt cacaaactcg tcaacatcat cataaccgct tttgtcaggg
     1921 ttgttgttgt attcagatct gttgtgtgaa ttgagaaacc gttgatcgct gtaaggcccg
     1981 aaatcaaacc accaggacga aggatcgacc gaccagtctg atattttctg ataataccac
     2041 acagggaaaa taacgctcag aaccgatatt gcataaactg aataaagaat tgattttctt
     2101 ttgttattct caattatttc aacaatttga ttaaacatga ccgatgcaaa gagagccata
     2161 attataaaac aggggagaag aaactggaac tggtcccagg tattgtagaa ctgaaagaat
     2221 acgagcgtga caacaaaagg aagtatcaga gcagaataaa aaactttcca gtattcacct
     2281 ttgaaaggat ctttaagcaa tcctttgcgc tgaaggatca gcatcaaaaa gagagcgatg
     2341 aaaacattag cgacaaacat ccttatccct gaatcgtacc tgaaccatgt cagttttgtt
     2401 ccgaagatct caaagaccgc tccgttatgg aacaatattc cgaaagtcaa agcagtgaga
     2461 attgaaaggg tgagcagtgt cgaaagaaca gctttttcaa ccattgaccg gttttcagag
     2521 ggtttgacag tccttgaaat gaaccatccg gaaacgaagt ataacaaccc gagaacagta
     2581 agaaaataga aaggggtcgg gaactggaga aggtgatacc agaaagactg atagaagccc
     2641 gacatgttgt caaagtcgaa cattatattc ttgaactctc cacctgcacc gtggttgaat
     2701 gtctttgcaa ggtcatatcc ttttgctgcc catgttctga aaagaagaat gagataaggg
     2761 agaactccga gcagaagata gaacgaactg acaatgaata tccagtagaa cagaaacgat
     2821 cttttttcct taaatatttt ccggtaaaaa taggggagat gacccttttt ctgaatgtca
     2881 gaaaaactga acagaacaaa aagataacat ccaccccacc atacaccgtt ttgaacatgg
     2941 ttgaaaaaag ataaaccggc aagaaagaag gaaagataaa gcggccagat aacttttctc
     3001 tgtgtataat caaccagaca gatcagtatc acgattatca tgaacacatg gagaatataa
     3061 ttttccgcca ttgttgcatg ccaccagaca gaatggctca ccatgaaaat aaaagagctt
     3121 gcaacagtca caaattttct aagcccgagc ttgttcagca ggaaatagaa cgaaattaca
     3181 gaagcggcac cgaaaacaac agtgaacagg ttcgctctgt aaattaccgt tccgaaaggg
     3241 agtttcagaa aaagattgcc cacaagcatg gcaagattat gaagattagg atgaatgccc
     3301 agttcaagta tcaccattct gtgaaccatc agagcgacat caccgagggc catttttgtc
     3361 gccattgtaa agtaataaaa gaaaaacggt atcaggacaa accaatggct gataaaaaac
     3421 tttctgatgc ctctcatttt atctccgtct cagcttttaa taaattcagc cattgttttt
     3481 attgaataga gaactttctt tccggtttcg gtgtctttta tcttttttcc gtactctctt
     3541 tcaagaagga caatgagtga caatgcgtct attgaatcaa gaccaagccc ttcaccga
//
LOCUS       DAOOYB010000288         3589 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_31035_length_3589_cov_5.504810, whole genome shotgun sequence.
ACCESSION   DAOOYB010000288 DAOOYB010000000
VERSION     DAOOYB010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3589
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31035_length_3589_cov_5.504810"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..560
                     /locus_tag="PL056_12270"
     CDS             108..560
                     /locus_tag="PL056_12270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47839.1"
                     /translation="MKSIIITSLFMFLCSCDRTYDHGGLNWSHPSLEMNWYDAQEYCK
                     DLGRRLPTISELRGLIQDCPEAELGGSCRISDDCLSSIDCDYDKCCGCDFDWNDTGKY
                     DSLGFGSILWSSSVDSSDPDYVYLVFFDEASIGRHKKTYKCKVCCVKE"
     gene            712..>3589
                     /locus_tag="PL056_12275"
     CDS             712..>3589
                     /locus_tag="PL056_12275"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin like domain-containing protein"
                     /protein_id="HPM47840.1"
                     /translation="MRNIFLSVLSVFVLLSGCGDPENLPVITETSNEFRVFMEKKEAG
                     LQVLKDPRFGLIPSPVSNAHLLKQMIIKPVDVNLKNATDPAYDLRDYGRVTSVKNQGD
                     CGSCWTFAAMASAESSLMPSETNDFSENNMKNRHGFDWGPCDGGNYDMAAAYFMSWKG
                     PVNEVNDPYGEYDDTSPVLSPVKHVKNYLSVSSANIASIKEAVVEHGVVFADMHFDDP
                     YFKSSTNGYYYSGSSDINHGIAIVGWDDNFSKTGFNSAPLSDGAWIVKNSWGTGWGDS
                     GYFYISYEDSKINNDCYVYQATDIEYDNLYSYDPFGRISVSGYGADTAWIANVFTASS
                     DEILKAVSFYANSHGAEYTVRIYTGVTSTPITGGTLRSEKSGTLERAGYNSIVLDDEV
                     NLMVGTKFSVVIKVRTPGTNDVIPLEMPYAGYSSGATANIGESYITYDDGGNPGAWTD
                     VAGWITNTNINIKAYTIDQVEAESGLVINEIDYDQPSTDTAEFIELYNAGPDIPDLSS
                     YSIQLINGTGGGASVYQTISLPAVSLTSGEYYVICANSTTVANCDLDVNPDENLIQNG
                     APDAIGLYRGLTLIDAVSYEGDSGVPYTEGSGVGLVDDSSIADRGISRYPNGVDTDIN
                     NVDFSFRCITPGVANIEKNSNCLCGNGVVDAGEDCEPGEGSCCTSECRFVAIDTVCSS
                     ATHPCELDAKCTGASSSCPANPPAENGTDCDLDSSLCTQDKCQGGTCNAGVMVVCDDS
                     KPCTDDTCNPSTGTCQYVNDNSNSCTDGNGCTVGDSCNSGSCVSGAAANCDQNCTNNE
                     PGYICTCGSGYILNVDGHTCDDINECTLGIGNCDANATCANTTGSFTCTCNSGYTGNG
                     TVCSDIDECTLGIDNCDANATCANTAGSFTCTCNSGYTGSGVTCSDVDECDLNTDNCD
                     QNCENTSGGFACSCEIGYELNIDGHTCDDIAECGNGIVEPGEECE"
BASE COUNT         1043 a          619 c          864 g         1063 t
ORIGIN      
        1 aaggaaaatg gatggtttac cagttcagag ctgttgcatt acaagcaata agagaccttg
       61 attgttattg tgaaaaagaa ggggtctgtc cgtttgggga atagaatatg aaaagtatta
      121 tcatcacctc tttatttatg tttctttgca gctgtgaccg tacctatgat cacggtggac
      181 taaattggtc ccacccttca cttgaaatga actggtatga tgcccaagaa tattgtaagg
      241 atctcggcag aagattgcca actataagtg agcttcgtgg attaatacag gactgccctg
      301 aagctgaatt agggggttct tgcaggataa gtgatgactg tctttcttcc atagactgtg
      361 attacgacaa gtgctgtggt tgtgattttg attggaatga tacaggcaaa tatgattctt
      421 tagggttcgg cagtattctg tggtcatctt cagttgattc aagtgatcct gattatgtat
      481 atctcgtttt tttcgacgaa gcaagcatcg ggagacataa aaaaacttat aaatgcaaag
      541 tttgctgtgt gaaagagtaa aaaaacagtc taaaaaagtc gatgtttatt aaatagatta
      601 gttataactc gcagtttttc atgtggaata agcaaaagca ttactgatca gtaactttga
      661 ctaaatctca ggaaacaggt aaaatattcc gtaaattttt gggagatgaa tatgagaaac
      721 atttttttat cagttctttc tgtttttgtt ttattgtctg gatgcggtga tcctgaaaac
      781 ttaccggtta ttactgagac atcaaatgaa ttcagagttt ttatggaaaa aaaagaagca
      841 ggtttgcagg ttctaaaaga tccgaggttt ggtctgatcc cttctccggt ttcaaatgct
      901 catcttttaa agcagatgat aattaaacca gttgatgtga atttgaaaaa cgcaacggat
      961 cctgcttatg atctcagaga ttacggcaga gtcacttctg ttaaaaatca gggtgattgc
     1021 ggaagctgct ggacttttgc cgcaatggca tcggcggaat catcattaat gccgtcggaa
     1081 accaacgatt tttctgaaaa taatatgaaa aacagacacg gttttgactg gggcccgtgt
     1141 gatggtggaa attatgatat ggcggcggct tattttatga gctggaaagg tcctgttaat
     1201 gaagtgaatg atccttatgg tgaatatgat gatacttctc ctgttctttc tcctgttaag
     1261 catgtaaaaa attatttgag cgtttcaagt gctaatattg catcaataaa ggaagctgtt
     1321 gttgaacacg gtgttgtttt tgctgatatg cattttgatg atccatactt taaatcatcg
     1381 acaaatggat attattattc aggttcttct gatattaacc acggaatagc tattgtggga
     1441 tgggatgata atttcagtaa gaccggtttt aattcagctc ctttgtcaga tggtgcgtgg
     1501 attgttaaaa acagttgggg aacaggctgg ggcgatagcg gatactttta tatttcctat
     1561 gaagactcta agatcaacaa cgactgctat gtctatcagg caactgatat tgagtatgac
     1621 aatctttatt catatgatcc gtttggaagg ataagtgttt cggggtatgg agctgataca
     1681 gcatggattg caaatgtttt cactgcttca agcgatgaaa ttctgaaagc tgtcagtttt
     1741 tatgcaaatt cgcacggagc agaatataca gtgagaattt atacaggtgt aacaagtacc
     1801 ccgataaccg gcggaacttt gcgttcagaa aagtcgggaa cacttgaaag agccggctat
     1861 aactcgatcg ttcttgatga tgaagtcaat ttgatggttg ggacaaaatt ttcagttgta
     1921 ataaaagtcc gtactccggg aaccaatgat gttattccgt tagaaatgcc ttatgccggg
     1981 tattcaagcg gggcaacagc aaacattggt gaaagttata tcacctatga tgatggggga
     2041 aaccctggtg catggaccga tgtcgctggc tggattacaa acacaaatat caatatcaag
     2101 gcatacacca ttgatcaggt tgaagctgaa agcggtcttg taattaacga aatagattac
     2161 gatcagccgt caaccgatac tgctgaattt atagaacttt ataacgcagg acctgatatt
     2221 cccgatcttt cgagttattc tatccagctt ataaatggaa ctggaggcgg agcatcagtt
     2281 tatcagacaa tatctcttcc cgctgtttct ctgacaagtg gcgagtatta tgtcatctgt
     2341 gcaaattcta ctacggttgc aaattgtgat ttggatgtca accctgatga aaaccttata
     2401 cagaacggcg ctccggacgc aatcggtctt tacagaggtt taactttaat agatgctgtt
     2461 tcgtatgaag gtgattcagg agttccttat actgaaggtt ccggtgtcgg tcttgttgat
     2521 gactcatcga tagctgacag aggtatttca aggtatccaa atggcgttga tacagatata
     2581 aacaatgttg atttttcgtt tagatgcata actcctggtg ttgcaaatat tgaaaagaac
     2641 agtaactgcc tgtgtgggaa cggtgttgtt gatgcaggtg aagattgtga gccgggtgag
     2701 ggatcatgct gtacctcgga atgcagattc gtagcgatag ataccgtctg ctcatcggca
     2761 acccatccat gtgaacttga tgcaaaatgc acaggagctt cgagtagttg tcccgcaaat
     2821 cctcctgcgg aaaacggaac ggactgtgat cttgattcca gtctgtgtac tcaggataaa
     2881 tgtcagggcg ggacatgcaa tgcaggtgta atggttgtgt gtgatgattc aaaaccgtgt
     2941 actgatgaca catgcaatcc atcgaccggt acatgccagt atgtgaatga taattcaaat
     3001 tcgtgtacgg atggcaatgg ttgtactgtc ggtgacagct gtaattcagg ttcgtgtgtg
     3061 agcggtgcgg cagctaactg cgaccagaac tgcacaaaca atgagccggg ttatatttgt
     3121 acctgcggtt caggatatat acttaatgtc gacgggcata cttgtgacga tatcaacgaa
     3181 tgcacgctcg gtataggcaa ttgtgatgca aacgcgacat gtgcaaatac cacaggatca
     3241 tttacctgta catgcaacag cggttatacc ggaaacggca cagtatgtag tgatattgac
     3301 gaatgtacgc tcggtataga caactgcgat gcaaacgcaa catgtgctaa cacagcagga
     3361 tcattcacct gtacatgcaa cagcggttat accggaagcg gtgtgacctg tagtgatgtt
     3421 gatgaatgtg atctcaatac cgataactgt gatcagaatt gtgaaaatac ttcaggcgga
     3481 ttcgcttgca gttgcgaaat agggtatgaa ttgaacatag acggacacac ttgtgatgat
     3541 attgctgagt gcggcaacgg cattgttgag ccgggtgagg aatgtgagc
//
LOCUS       DAOOYB010000289         3575 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_31184_length_3575_cov_14.728125, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000289 DAOOYB010000000
VERSION     DAOOYB010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3575
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31184_length_3575_cov_14.728125"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1594)
                     /locus_tag="PL056_12280"
     CDS             complement(<1..1594)
                     /locus_tag="PL056_12280"
                     /inference="COORDINATES: protein motif:HMM:NF014896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HPM47841.1"
                     /translation="MKHSVVFFLAVSIVIAGCAASAASDGKKESAPVAKWNYPETERD
                     VVTDEYFGTKVEDPYRWLEENENSEVQKWTEAQNNFTEKTIFGWDQTVKMEKRLSQIW
                     NFDKMQTPVRKGDKLFYRFQQGLQNQPALFVQMADGTKKELIDPNTINAEGTTAMDWW
                     YASPKGKFIAYGLSEQGSEQSVLHVLNVETGEKADEPIDGCRYASVGWLADESGFFYS
                     RKLDGDKPGEIDTEQSIRFHKLGTPSENDEIIAKATLKEGIIVSTVDNEGKWLLISEY
                     MGSSGKARLEIYNIEKKEKKPVVDNFDDIYEGEVYAGHLYLKTSKDNALNWKIEKVNC
                     ETLEWVTVVPEHEKDVIEYFGFSNGKIVVLKMHDVASRITVVDIDGKNEIEIKLPVLG
                     NVQGMSSEHSSDEMFISFSSFAYPPVVFHHSAGELKTFWKADVPVDVKDIVTEQVFYE
                     SKDGTKIPMFIIYKKGLEKNGSNHTFLRGYGGFNVSYPMYFSATNAAWIETGGIMAIA
                     NIRGGGEYGEKWHQSGMLDKKQN"
     gene            complement(1610..3313)
                     /locus_tag="PL056_12285"
     CDS             complement(1610..3313)
                     /locus_tag="PL056_12285"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPM47842.1"
                     /translation="MIKKIVSVLMLILPLTITAGEVYFYSAGKKIVLEENFSNSFHSD
                     ETPAYRFQKGHAIRANRNLFVKIPGAPSEKEARKWCESNGLKFIKQYKYIPSWYLVSY
                     SGNTIKKSAELVEGRIVEQAEPSFYIPLELKTYVPADEHFFKQWHLHNWGGTAEGLTG
                     DDHAHVANAWTVMRHFTGKMGEGVKLAIIDDGFDLDHEDLAGRFLPGHDFGDNDDLPY
                     PGPQDAHGTCCAGVAGAKTNNEVGVSGACPDCMIIPIRMNMNSTAGTLDSIAIESFTW
                     AADNGADIISNSWGPPDGGGAVAMGQTLKDLVTNLTTSGRNGKGIIILFAAGNGGESI
                     EGDGFASNPDVFAIGASTASGKRSSYSDFGSSLDFLAPSNDVDNSGGDGWSGSYIDGI
                     WTTDNTNGGYNPGQLAGDTTGKYVSEFGGTSSACPLGAGITGLVLAANPDLTKDQVYD
                     IFKTTSDKVSKGEMNPMENDYDANGFSTHYGWGRLNACEAVKMAFDMAGKNVENIVCG
                     EDLETSDNDPVNPDKDNPANDNETLNDNETTDDPNLENDDVEPVGKRADDGCSCSFVN
                     I"
BASE COUNT          873 a          874 c          674 g         1154 t
ORIGIN      
        1 tgttctgttt tttgtcgagc attcctgact ggtgccactt ttcaccatat tctccaccgc
       61 ctctgatatt ggctatcgcc attattccgc ctgtttcgat ccatgcggca ttggttgccg
      121 aaaaatacat tggataactg acattaaacc cgccgtatcc ccttaaaaaa gtatgatttg
      181 acccgttttt ctcaagacct ttcttataaa ttatgaacat cgggatcttg gttccgtcct
      241 ttgattcata aaatacctgt tctgtaacga tatccttcac atcaacagga acatcggctt
      301 tccagaatgt tttgagttct cctgcagaat ggtgaaatac aactggaggg tatgcaaagc
      361 ttgagaaact tatgaacatc tcatcactgc tgtgttcgga tgacattccc tgaacatttc
      421 ccaaaaccgg aagctttatt tcgatctcat tcttaccgtc aatatcaaca actgttattc
      481 tgcttgcgac atcgtgcatt ttgagaacga ctatttttcc gtttgaaaat ccgaaatatt
      541 ctatgacatc tttttcgtgt tcaggaacaa ctgtgaccca ttcaagagtt tcacagttca
      601 ctttctctat tttccagttc agtgcattgt cctttgatgt tttgagataa agatgtccgg
      661 catagacctc tccttcataa atgtcatcaa aattatccac aaccggcttt ttctcttttt
      721 tctcaatgtt atatatttca agccgcgctt ttccgcttga acccatatat tcagagataa
      781 ggagccattt tccttcgttg tctacagtgc ttacgattat cccttccttc aatgtggctt
      841 ttgcgattat ctcatcattt tcagatggtg ttccaagctt gtggaatctt atgctctgct
      901 ctgtgtcgat ctctcctggc ttgtctccgt caagtttcct tgaatagaaa aacccgcttt
      961 catcagcaag ccagccgaca gaggcatatc tgcagccgtc aatgggttca tcagcttttt
     1021 caccggtttc gacattcaaa acatggagaa ctgactgctc cgagccctgc tcgctcagtc
     1081 cgtatgcgat gaacttccct ttgggtgaag cataccacca gtccattgca gtcgtccctt
     1141 cagcatttat ggtgtttgga tctataagtt ctttttttgt gccgtcagcc atttgcacaa
     1201 aaagagccgg ctgattctgc aaaccctgct gaaatctgta aaaaagctta tctccttttc
     1261 ttaccggagt ctgcattttg tcaaaattcc agatctgaga gagccgtttt tccattttta
     1321 ccgtctgatc ccagccaaaa attgtttttt cggtgaaatt gttctgtgcc tctgtccatt
     1381 tctgaacttc agaattttca ttttcctcaa gccagcggta aggatcttcc actttcgttc
     1441 cgaaatactc atcggtaacg acatctcttt ctgtttcagg ataattccac tttgcaaccg
     1501 gggcggattc tttttttccg tcacttgccg cacttgccgc acagcccgca ataacaatgg
     1561 aaactgccaa gaaaaaaact actgaatgtt tcataattct cctttattat caaatattta
     1621 caaaactgca cgaacatccg tcatctgctc tttttcctac cggctcgaca tcatcatttt
     1681 caagattcgg atcatcggtt gtttcattgt cgtttaaggt ttcattgtca tttgccggat
     1741 tatctttatc gggatttacc ggatcattgt cagatgtttc aaggtcttct ccgcaaacta
     1801 tgttctcaac attttttcct gccatgtcga aagccatttt caccgcttca caggcattta
     1861 accgtcccca gccgtaatgg gtgctgaaac cgttcgcatc gtagtcgttc tccatgggat
     1921 tcatttcacc cttgctaact ttatcggaag tcgttttgaa aatatcatag acctgatcct
     1981 ttgtaagatc gggatttgcg gcaagaacaa gccctgttat ccctgctccg agcggacacg
     2041 ccgaagatgt tccgccgaat tcgctcacat attttcctgt tgtatcaccg gcaagctgtc
     2101 ccggattgta tccgccgtta gtgttgtccg ttgtccagat cccgtcaata taactaccac
     2161 tccagccgtc acctccgctg ttatcaacat cgttgctcgg agcaaggaaa tcaagtgaac
     2221 tgccgaaatc tgaatagctt gatcttttgc ctgatgcagt tgaagcgccg atcgcaaaaa
     2281 catccgggtt gcttgcaaag ccgtcacctt cgatgctttc gccgccgtta cctgccgcaa
     2341 aaaggatgat tatccctttt ccgtttctcc ctgaagttgt caggtttgtt acaagatctt
     2401 tgagcgtctg gcccatggcg actgctccgc ctccgtccgg cggaccccaa ctgtttgaaa
     2461 ttatgtctgc accgttatct gccgcccatg tgaaagattc tattgctatt gaatcaagtg
     2521 ttcctgccgt gctgttcata ttcatcctta tcggaattat catgcagtca ggacatgcac
     2581 ccgaaacacc gacttcgttg ttcgttttcg cacctgcaac tccggcacag catgtaccgt
     2641 gagcatcctg agggccggga taaggaagat catcattgtc tccgaaatca tgtccgggga
     2701 gaaatcttcc agcaagatct tcgtgatcaa ggtcgaatcc gtcatcaatt atcgcaagtt
     2761 tgaccccttc gcccattttt ccggtgaaat gtctcatcac ggtccatgca tttgcaacat
     2821 gggcatggtc atcacccgta agtccttcag cagttccgcc ccagttgtga aggtgccact
     2881 gtttgaaaaa atgttcgtca gcgggaacat aagttttaag ttcaagcgga atgtaaaaac
     2941 tcggttccgc ctgctcaacg atccttcctt ccacaagttc ggctgatttt tttattgtgt
     3001 tccctgaata tgaaacaaga taccacgaag gaatgtactt gtactgcttt ataaacttca
     3061 acccgttgga ttcacaccat tttcgagctt ctttttcaga aggagcccca ggtattttta
     3121 caaaaagatt tctgtttgcc ctgatcgcgt gacctttttg aaatctgtat gcgggggttt
     3181 catccgaatg gaaagaattt gaaaaattct cttcaagcac gatctttttc ccggctgaat
     3241 agaaataaac ttcgcctgcc gttatcgtga gcggcagaat gagcattaaa accgaaacga
     3301 tctttttaat catcataatc tccaagttca agaatgaatt caataaactt atgacaattt
     3361 acataaaagc aattaatttg ccagattttc agctttcagc ccgtgctttc agttaactaa
     3421 attgactttt ttcaatattt gatgattatt tacgaagatc gtttttgaat ctttggagga
     3481 tgaagatgaa taaaaaaggt ttttcgcttt ttgcggtact gatccttctt ctggtgtcaa
     3541 caggttttga gccgcttctg gcaaagaaaa aaatg
//
LOCUS       DAOOYB010000290         3547 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_31519_length_3547_cov_8.945876, whole genome shotgun sequence.
ACCESSION   DAOOYB010000290 DAOOYB010000000
VERSION     DAOOYB010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3547
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31519_length_3547_cov_8.945876"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            177..1613
                     /locus_tag="PL056_12290"
     CDS             177..1613
                     /locus_tag="PL056_12290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47843.1"
                     /translation="MKKIVYVLILVFSVLFVLSCSSKKDDNESNDSDSTGDGPKCGNG
                     VIDEGEICDGNIPCWQAGHFYPEFEAKCNADCTAYDTVQCQKRPDDDLCGNFVKDDKE
                     TCEQGETKPCTELPGSYETGDAACNRWCTGWDPTNCSTGGNKTCAQIFTCVDACTDDA
                     CIEGCRGQGSEQGLELFNALYECWTGNCASGADKEECMKESCNDEYYSCYPNEKCGNG
                     VIDEGEKCETLETKPCQDFGEDWQPINEAICNSTCTDWDMYACIDKDALTCYQVYECV
                     EKCDNSECEEECIGKTWPAAKAKYDTMLECLDKECSDAPVMEVCMNEFCKFQTDACKT
                     HLTCGNSNIDMYENCEKGESVDCGTIEDEQGEAMYEAGTANAFCNTNCTEYITLMCFK
                     FCSCSEIKTCVEQECGGYSNSADADCLEECESWGSQLGEGEASGWRKFIQSCSDSDGN
                     KAFDSKTCVDEANKNYDCGVSDHEKCPY"
     gene            1645..3417
                     /locus_tag="PL056_12295"
     CDS             1645..3417
                     /locus_tag="PL056_12295"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HPM47844.1"
                     /translation="MKKILAGTVFISLCIAALYVYAEILINRKIDETVSKAVMLLERK
                     TGLDMDVSDTGFSLIKPEVYAGNIKISKQGSFVAAFSDCRVSDVVKIYFGKKKDVNIV
                     CIGSAVNGDQIFSAAKSYISTEKAQTPGSSGGKNEEYSLDLEINDMTLSFDGKTRSVN
                     FGMELNGSLGKITVKDQVNLKGRGSAEVVFNTSFEKVDLLLNAIPLDMVRDFVILKTG
                     VKNISGTVDGFINILRKSTDISADIDISVKGLNIEHPLIDWQPYKLSFFRFSGVAVAD
                     IGKKSLKSENSKISLGGIDGSFSAKKDDTGVSFAVDINKVPLNKLETLVHNDVFKGYL
                     FDGDIDLKVTYSKEGDAEPVFSVTGEVVEPLQISDRLNYLREPFPFNFIDRNDQPVSF
                     VVGAGNRDFIALDYIPEHVMWAVIVSEDAGFFMHKGVDFEEMSAAVKDNIKKKKMRGG
                     STITQQIAKNLFLKREKTLLRKFREVILAIELDATLSKKRLLEIYFNIVEWAPGIFGI
                     SNAAWYYFGKPVYMLTPLEGAYLASVIPGPYRYNYQFQNGKVSEKWIENLHRILNIMN
                     ETGHLTFQEYIDSVKEELLFRPKE"
BASE COUNT         1140 a          608 c          846 g          953 t
ORIGIN      
        1 atgccgttga aatatttcag tttcacgaag atcacacctc aatacttaat tcagaagaag
       61 tatttaaaaa actcattgaa gtcattgaga attgaatttt ctcttgacta cttttgaaca
      121 atttttagaa tctggtgagt gcaggtgtgt taaattgctt ttttatggag ataaaaatga
      181 aaaaaatcgt ttatgttctt attttagttt tttcagttct ttttgttttg agctgttcat
      241 ccaaaaagga cgacaatgaa tcaaatgaca gcgatagtac cggtgacggt cctaaatgcg
      301 gcaatggcgt gatagatgaa ggagaaatct gtgacggcaa cattccatgc tggcaggcag
      361 gccatttcta tccggaattt gaagcaaagt gtaatgccga ttgtactgct tatgatacag
      421 tacaatgtca gaaaagacct gatgatgatc tttgcggaaa ttttgtaaaa gatgataaag
      481 aaacatgtga acagggtgaa acaaagccct gtactgaact ccccggtagt tatgagacag
      541 gtgatgcggc atgcaacaga tggtgcaccg gatgggatcc tacaaactgt tcaacgggtg
      601 gcaataagac ttgcgcacag atatttacat gtgtcgatgc ctgtactgat gatgcctgta
      661 ttgaaggatg cagaggccag ggaagcgagc agggacttga actgtttaat gcgctttatg
      721 aatgctggac cggcaactgt gcaagcggtg cggataaaga ggagtgtatg aaagaaagct
      781 gcaacgatga atattattcc tgctatccga acgagaaatg cggtaacggt gtgatagatg
      841 aaggagaaaa atgcgagaca ttggagacta aaccatgtca ggactttggt gaagactggc
      901 agccgataaa cgaagctata tgcaattcaa cctgtactga ctgggatatg tatgcatgta
      961 tagacaaaga tgctcttacc tgttatcagg tctatgaatg tgttgaaaaa tgtgataatt
     1021 ctgaatgtga ggaagagtgc attggtaaga catggcctgc tgcaaaggca aaatatgaca
     1081 caatgttaga gtgccttgat aaagaatgta gcgatgctcc tgttatggaa gtctgcatga
     1141 atgagttctg caagttccag actgatgcat gcaaaactca tcttacatgc ggcaacagca
     1201 atattgacat gtatgagaac tgtgaaaaag gtgaatcagt agactgcgga actattgaag
     1261 atgagcaggg cgaagcaatg tatgaagcag gaacggcaaa tgctttctgc aacaccaact
     1321 gtacagaata catcacactg atgtgcttta agttctgcag ctgttcagag atcaagacat
     1381 gtgttgaaca ggaatgtggc ggctacagca acagtgcgga tgccgattgt cttgaggaat
     1441 gtgaatcatg gggaagtcag ctcggagaag gcgaagcaag cggctggaga aaatttattc
     1501 agagctgctc agacagcgac ggaaataaag ctttcgacag taagacctgc gtcgatgaag
     1561 cgaacaagaa ctacgactgc ggtgtatctg atcacgaaaa gtgcccatac tgataataat
     1621 tcagatagtt aatatcctag ccaaatgaaa aagatcctgg ccggaacggt gtttatatcc
     1681 ctgtgtattg ctgcactgta tgtttacgca gagattttaa taaacagaaa gatagatgaa
     1741 actgtctcaa aagctgtcat gctgttagaa agaaaaacgg gtcttgacat ggatgtttcc
     1801 gacacaggtt tcagtttaat aaagccagaa gtgtatgctg gaaatataaa aatctccaaa
     1861 cagggctctt ttgttgcagc tttttcagac tgccgggttt ctgatgtggt aaagatctat
     1921 ttcggaaaga aaaaggatgt taacattgtc tgcatcggat ctgccgtgaa cggtgatcag
     1981 atattcagtg ccgccaagag ttatatttca actgaaaaag ctcaaacacc aggatcatcg
     2041 ggtggaaaga atgaggaata cagcttagat ctggaaatta atgatatgac tctatctttt
     2101 gatggaaaaa cgaggtccgt caatttcgga atggaactga acggatctct tgggaagatc
     2161 accgttaaag atcaggtaaa tttaaaggga aggggatcgg cagaagtagt tttcaacaca
     2221 tctttcgaaa aggtggatct tcttttgaac gctattccgc ttgatatggt aagggatttt
     2281 gttattttga agacaggtgt gaagaatatt tcaggaacgg ttgacggttt tatcaacata
     2341 ttgagaaaga gcactgatat ttcagcagat attgatattt cagtaaaggg cctgaatatt
     2401 gaacatcctc tgatcgactg gcagccgtat aaactttctt ttttcaggtt ttcaggggtt
     2461 gcagttgctg atatcgggaa aaaaagttta aaatcagaaa attcaaaaat atctctgggc
     2521 ggcatcgacg gcagtttttc tgcaaaaaag gatgacacag gcgtctcatt tgcagtagat
     2581 ataaacaaag ttcctctgaa caagctcgaa acactggttc ataatgatgt tttcaaggga
     2641 tatctctttg acggagacat tgatctgaaa gtgacatatt caaaagaagg tgatgcagag
     2701 ccggtatttt cagtgacagg cgaagtggta gagccattgc agatatcaga ccggctgaat
     2761 tatcttaggg agccgtttcc gttcaatttt attgacagaa acgatcagcc ggtttcgttt
     2821 gtggtcgggg cgggcaacag ggacttcatt gcacttgatt atattccgga acatgtgatg
     2881 tgggcggtca ttgtttcaga agatgccggt ttcttcatgc ataaaggagt cgattttgaa
     2941 gaaatgtctg ctgcagtaaa ggacaacata aagaaaaaga agatgagagg cggctcgaca
     3001 ataacacagc agatagcgaa aaacctcttt ctgaaaagag aaaaaacact tctgagaaaa
     3061 ttcagggaag tgatccttgc aatagaactt gacgcgacac tttccaaaaa acggcttctt
     3121 gagatatatt tcaatatagt tgaatgggct ccgggaattt tcggaatatc aaatgccgca
     3181 tggtactatt tcggaaaacc tgtctatatg cttactccgc ttgaaggtgc ttatcttgct
     3241 tccgtaatac ccggtccgta cagatacaat tatcagttcc agaacggaaa agtaagtgaa
     3301 aaatggatag agaatcttca cagaatactc aacataatga atgaaaccgg acatctcact
     3361 tttcaggaat atatcgactc agttaaagaa gaacttctgt tccgtcccaa agaataaaaa
     3421 atcataacca gtgaatatga aggtggtgat ataagttact cctcaaaagg gcagatgccc
     3481 tcttctttac agtagcagtc catgtctctt aaaatatacc ctgctttata catcggctgc
     3541 cagaaaa
//
LOCUS       DAOOYB010000291         3535 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_31657_length_3535_cov_7.777586, whole genome shotgun sequence.
ACCESSION   DAOOYB010000291 DAOOYB010000000
VERSION     DAOOYB010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3535
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31657_length_3535_cov_7.777586"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="PL056_12300"
     CDS             complement(<1..366)
                     /locus_tag="PL056_12300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47845.1"
                     /translation="MWRRAHLPAQKLAIIDGLLPITADEYKKAVIFFCGDDDESVWKK
                     AIEKLKDFSIPDIRKNIDQSMPEKSVLALTKLAGERKAASILIALMETGKMHVEWILD
                     YVMIQDEDLWKMLITHKDFV"
     gene            complement(372..1139)
                     /locus_tag="PL056_12305"
     CDS             complement(372..1139)
                     /locus_tag="PL056_12305"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009855.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HPM47846.1"
                     /translation="MNFIVDIGNSNTVIGIYREGLLVRRWRIATDRNLTVDDISSKIH
                     SLMMFSKIEISDIAFCIISSVVPTWNHVWERFSIEFLDVKPLFLKHTTTSGIKINIDN
                     PNEVGADRIANAVAAASLYKEGTFIIDSGTAITLDIVTPQKEYVGGAIMPGIIISMEA
                     MSLKTAKLPRFELDRPEKAIGRSTLAGLQSGVFFGFVGMIDRVIEESLKEVDFTPKII
                     STGGLAGALTTASKYISEFDRDLTLKGLNIIASLNRR"
     gene            complement(1140..2429)
                     /locus_tag="PL056_12310"
     CDS             complement(1140..2429)
                     /locus_tag="PL056_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003446055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HPM47847.1"
                     /translation="MNSEIKKLGSALIDFLDSSPTAFHATENIEKRLKKEGFSELDES
                     EPWKILPGEKYFVKRNSSSILAFIAGKEKPVKTGFRIAGAHTDSPLLKIKDNGENRSA
                     SCIRVTTEIYGGPLVYTWLDRDLGIAGRVMVKSKRGLKSMLVNINRPVAIIPSLAIHM
                     NREANKGFEFNPQNHLQVVLTADGSAKNVLKGLIASELKEKEENIAGMDMFVYDLQKG
                     VFTGNDSEFITCGRLDNLQMCHAILESFLSVKAPVATTVAAFYDNEEIGSKTLMGADS
                     TFTRTILERITALTSSGKDDIFRALSKSFLVSADGAHAHHPNFPEKHDPAYAPLMNKG
                     PVIKFSSSFRYATTAESSSEFEQICEQAGVPCQKIANRSDVPSGSTIGPMSSAALGVK
                     AVDVGNAMWAMHSIRETAGTLDHFYMKKALSHFYGRK"
     gene            complement(2452..3426)
                     /locus_tag="PL056_12315"
     CDS             complement(2452..3426)
                     /locus_tag="PL056_12315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47848.1"
                     /translation="MKRFLAVIMLFFVAAPVFATDAAPAEEKIEAPKYHSIFFKPKLS
                     VSFVDNRNVPGKTDGIYFRTDLDVVLNYKYAKDMHEFLIDLGIKEAISYTPLFDGFVI
                     AADVAKLEMRYNLLFNKWSGYYLKAGLETHFFPGYDYSTDAVDYNVNGQRKARAKKYE
                     LTTSGLPLFLYQGTGLFYRPIDTEESKMEFNLGLAAREVFVGDSLIVNDDAATPEKEL
                     TSMTDIYEAGGEAGFTYKGTAQEKKVGYDIMAKAIMPFYSKERENLGMSWGDIFQFEG
                     HIKLEFNINKYASFNYEFTIKRDFAIVKEWQMTNGLYLSLFYEIDKKM"
BASE COUNT          960 a          822 c          655 g         1098 t
ORIGIN      
        1 gacaaaatcc ttgtgtgtta taagcatctt ccacagatct tcatcctgga tcataacata
       61 atcaagaatc cattcgacat gcatttttcc tgtttccatc aatgctatca gaattgaagc
      121 cgctttcctt tcaccggcaa gctttgttaa tgcaagcact gacttttcag gcattgactg
      181 atcgatgttc tttctgatat caggtatcga aaaatctttc agtttttcaa ttgccttttt
      241 ccacactgat tcatcatcat ccccgcagaa aaatatgacc gcttttttat attcatccgc
      301 agtaattgga agaagtccgt caatgattgc aagtttctgt gccggtaaat gtgctctccg
      361 ccacatatat cctacctcct gttcaacgaa gcaattatgt tgagcccttt taaagtaagg
      421 tccctgtcaa attctgatat gtatttggat gccgttgtca atgcacccgc aagaccgcct
      481 gttgaaatta tcttcggcgt gaaatcgact tctttcaaag attcctcaat aactctgtcg
      541 atcatcccca caaaaccgaa aaagacacca ctctgaagcc cggcaagtgt tgatctgcca
      601 attgcttttt caggcctgtc aagctcgaag cggggaagtt tagcagtttt aagcgacatt
      661 gcctccattg atataattat gccgggcatt atcgcccctc cgacatattc cttctgagga
      721 gttactatat caagagttat cgctgtccct gaatcaatta tgaaagttcc ttctttatac
      781 agacttgccg ccgcgacagc atttgcgatc ctgtcagcac cgacctcatt agggttgtcg
      841 atattgatct ttatccctga tgtggtagtg tgtttaagaa aaaggggctt gacatcaagg
      901 aactctatcg aaaatctctc ccagacatga ttccatgtgg gaacaacgga acttattatg
      961 cagaaagcta tatcgctgat ctctatcttt gaaaacatca tgagtgagtg tatcttggaa
     1021 gaaatgtcat caactgtgag gtttctgtcg gttgcgatcc tccaccttct gacaagcaga
     1081 ccttccctgt aaatcccgat aacagtattg ctgttaccaa tatcaactat aaaattcatt
     1141 tatttcctgc cgtaaaaatg agaaagtgct ttcttcatat agaaatgatc aagtgttccc
     1201 gctgtctccc tgatcgagtg cattgcccac attgcgttac ccacatcaac tgcctttact
     1261 ccaagtgccg ctgaactcat cggacctatt gtgcttcccg aaggaacatc agatctgttt
     1321 gcgatcttct ggcacggaac acctgcctgt tcacatatct gctcaaattc agaactgctt
     1381 tcagctgtcg tagcatatct gaacgatgag ctgaatttta taacaggccc tttgttcatc
     1441 aatggtgcat aggcaggatc atgtttttca ggaaaattcg gatgatgtgc atgagccccg
     1501 tcggcactga caaggaaact tttggaaagt gctctgaaaa tgtcgtcttt accggaagaa
     1561 gtaagtgcgg tgatcctttc aagaattgtt cttgtaaatg ttgaatcggc tcccataaga
     1621 gtttttgaac cgatctcctc gttgtcataa aaagcggcaa ccgtagttgc taccggagct
     1681 ttaactgaaa ggaatgactc aagtattgcg tgacacatct gtagattatc aagccgcccg
     1741 catgttatga actcagaatc atttcctgta aaaactcctt tctgcagatc atagacaaac
     1801 atgtccatcc ctgcaatatt ctcttctttt tccttcagct cagacgcaat aagacctttg
     1861 agaacatttt ttgctgaacc gtctgcagta agaactacct gcagatgatt ctgggggttg
     1921 aactcgaaac ctttgttcgc ttcacgattc atgtgtattg ccagactcgg aattatagca
     1981 acaggcctgt tgatgttgac aagcatgctt tttaatcccc tttttgactt taccatcact
     2041 ctgcctgcga taccgagatc tctgtcaagc catgtataaa caagaggtcc accgtaaatt
     2101 tcagtagtca ctctgatgca ggaagcagac ctgttctctc cgttgtcctt gatcttcaaa
     2161 aggggactgt cggtatgtgc accggctatt ctgaagccgg ttttcacagg tttttccttc
     2221 ccggcaataa aggcgaggat cgatgaactg ttccttttaa caaaatattt ctctcccggt
     2281 aaaattttcc atggctcaga ttcatcaagt tccgagaacc cctctttttt caatcttttt
     2341 tcaatattct cagttgcgtg gaacgctgtt ggagaactgt caagaaaatc aataagggca
     2401 cttccaagtt ttttaatttc tgaattcatc tggatcctcc ggatatttat tttacatttt
     2461 tttatcgatc tcgtagaaca gcgacagata gagtccgttt gtcatctgcc actctttcac
     2521 tattgcaaaa tctcttttta ttgtgaactc atagttgaat gaagcgtatt tattgatgtt
     2581 gaattcaagt ttgatatgtc cttcaaactg gaagatatca ccccagctca ttccgaggtt
     2641 ttccctttcc ttgctgtaaa aaggcattat agcttttgcc attatatcat aacccacctt
     2701 cttttcctgt gcagtccctt tataggtaaa ccctgcctct ccgcctgctt cataaatatc
     2761 agtcattgaa gtgagctctt tttcaggagt cgcggcatca tcatttacga tgagcgaatc
     2821 accgacaaaa acctctcttg ccgcaagacc gagattgaat tccatcttgc tttcctctgt
     2881 gtctatcggt ctataaaaaa gaccggtgcc ctgatacagg aacagcggaa gacctgaagt
     2941 tgtcagttca tattttttgg cccgtgcttt tctttgaccg ttcacattgt aatcaaccgc
     3001 atccgtactg tagtcgtatc cggggaaaaa gtgggtctca agtccggctt taagataata
     3061 accggaccac ttgttgaaaa gaagattata gcgcatttca agcttagcca catccgccgc
     3121 gatcacaaaa ccgtcaaaaa gaggcgtgta gcttattgcc tccttaattc ccagatcaat
     3181 aagaaattca tgcatatctt tcgcatactt gtaattcaaa actacatcaa gatcagtcct
     3241 gaaatatatg ccgtccgttt tgcccggaac atttctgttg tcaacaaaac ttactgaaag
     3301 tttcggtttg aaaaagatcg aatggtactt tggtgcttca attttttcct ctgcaggtgc
     3361 ggcatcagtt gcaaaaacag gtgccgcaac aaaaaacagc atgataactg ccagaaatct
     3421 tttcacaatt cacctccaac gcaaaaagtt cgcagtaatt atagacatat ttaaaaaata
     3481 atgcaacctt gcatatacga attaacttta ctgataaaac tgatttgtta atatc
//
LOCUS       DAOOYB010000292         3511 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_31982_length_3511_cov_7.260995, whole genome shotgun sequence.
ACCESSION   DAOOYB010000292 DAOOYB010000000
VERSION     DAOOYB010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31982_length_3511_cov_7.260995"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..1104)
                     /locus_tag="PL056_12320"
     CDS             complement(94..1104)
                     /locus_tag="PL056_12320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47849.1"
                     /translation="MKYFAVIILIFWLCSACNGKSQNHDFQDLPDLHDFDNENEYFEE
                     TEFIDEQNELEYIEEVTESESDSDIDETLKPDDAEYVCTCDTVEGDEDGDGIPNTVEG
                     CADSDSDGIPNCMDSDSDNDRIPDSEECPEFPCRDTDQDGFPDYLDRDSDNDGLADGY
                     EIELGLDHLNPDTDGDGTDDGTEIYIKTDPFDSTEPPDGVIYVYIIEGSYKTIKLSII
                     SGVDDLVPEQEYVQKDITIEADKDYGCQIYGHYYNILAGFKTVSAVPENGIDSFDEKT
                     FYNVMKNTEITFNAAVAFSFCSYETEMAYGKYGVKININFVSGGIILKSQPVFVILKA
                     LE"
     gene            1482..1829
                     /locus_tag="PL056_12325"
     CDS             1482..1829
                     /locus_tag="PL056_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008504261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HPM47850.1"
                     /translation="MYREIELMEKENGKCEALEFIEKMDEKTQMKIEYVFKLVEEILI
                     VPAEYLKKLRGQIYEIRVQSGSNIYRFLCFFHKGNLVVITHGFQKKSRETPSKEIDRA
                     EKLREKYLAKNGG"
     gene            1832..2104
                     /locus_tag="PL056_12330"
     CDS             1832..2104
                     /locus_tag="PL056_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016957713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPM47851.1"
                     /translation="MKFKKHLEKKYDTEAKMSEFLKGYEEFKLGVMLKEERKKANMTQ
                     SQVAERMGTQKEAISRIENHSKNINIETLLKYAAALGLKVKIIFES"
     gene            complement(2266..3285)
                     /locus_tag="PL056_12335"
     CDS             complement(2266..3285)
                     /locus_tag="PL056_12335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47852.1"
                     /translation="MTSCKTTADCIRSQYCDYENPKEDLNTGTLSYFCKDRVKCLKES
                     DCPMNWRCLIEEGFCITDKEAEGVLCKSDADCMTFPNVKCNLVTGQCYDPDAGDTGDT
                     GNTGNTGNTGNTGDTGNTGDTGNTGNSGNTGDTGNTGDTGNTGNSGNTGDTGNTGNTG
                     NSGTIIVSEDFEDGGTNWTIAGVWQIGAPTNGPAAAVSGSNVAATNLTGNYTDNANDI
                     LILNTQITIPVSGTHVVEFMAWVNIEGGGYSPVDYTDILIKRDTDTWEAISGIYLMAN
                     TPSPLDALDNTRTKITKKLGTSYYKFSGDLSSYKGETVQIGFRFKSDPIDNLEGIYID
                     DVVVK"
BASE COUNT         1097 a          694 c          624 g         1096 t
ORIGIN      
        1 tttatctctt cccagcagat ttatcgccga aattgatgaa aaattttatg agattttaag
       61 gtgacgaact tcagattttt cagctctcac cgatcattca agtgctttaa gaattacaaa
      121 aaccggttgg cttttcaaaa taatgccgcc actcacaaag ttaatattta tttttactcc
      181 atacttgccg taagccatct ctgtttcata actgcaaaag ctgaatgcaa cagctgcatt
      241 aaatgtgatt tcagtatttt tcataacatt gtaaaaggtc ttctcatcaa atgaatcaat
      301 tccattctcc ggaacagctg aaaccgtttt aaatcctgca agaatattgt aataatgtcc
      361 ataaatctga catccataat ctttatcagc ttcaatcgtt atatcttttt gaacatattc
      421 ctgctcagga accagatcat caactcctga tattattgaa agcttaatcg ttttgtatga
      481 tccttcaata atatagacat aaataacacc atcaggcggc tctgtgctgt caaaaggatc
      541 tgttttgata tatatttctg tcccgtcatc agttccgtct ccatcagtat caggattgag
      601 atgatctaat cccagctcta tttcatatcc atctgcaaga ccatcattgt cactgtcacg
      661 atcaagataa tcaggaaacc cgtcctggtc agtatctctg catggaaatt cagggcattc
      721 ttctgaatca ggaatgcgat cgttatccga atcactatcc atgcagttcg gtattccatc
      781 gctatcacta tcagcacatc cttctacagt attgggaatt ccatctccat cttcgtcgcc
      841 ttcaactgta tcgcaagtgc agacatattc agcatcatcc ggcttaagag tctcatcaat
      901 gtcggaatct gattctgatt ctgtaacttc ttcaatgtat tctaattcat tctgttcatc
      961 aataaactct gtttcttcaa aatactcatt ttcattgtca aaatcatgta aatccggcaa
     1021 atcctgaaaa tcatggttct gactttttcc gttacatgcc gaacacaacc agaaaattaa
     1081 aataattact gcaaaatact tcataaaacc tcaaatgaaa gcatttaaaa actttgaaag
     1141 tgtatctgaa aaatccattt tagtcaaata gctcaagaaa aaagaagggt cagggcacaa
     1201 tatttatata tatttttacc gtaaaattct tcttaactcg ctgaattgca acagcttttc
     1261 tcctgatttc cgctgactaa attatttaca tcacatttct tgcaaattta atttaattac
     1321 tgtattatct attggcgact aagtttttgg agttgaaaag cacaatgaac cacaaaaaca
     1381 attgaaggtg aaaagttgac agtaatgtta actttttcat tgacagtggt taacaaaggt
     1441 gttaacattg ttgtgggttc agccatttca ggaggttgag cttgtatcgg gaaatcgagt
     1501 tgatggaaaa ggaaaacgga aagtgtgaag cacttgagtt cattgaaaaa atggatgaga
     1561 aaacacaaat gaaaattgag tatgtcttta agctggtgga ggaaattctg atagtccctg
     1621 ccgaatatct taaaaaactc agaggacaga tatatgagat aagggttcag tcagggagca
     1681 acatttacag gtttctgtgt ttttttcata aaggaaatct ggttgtgatt acacacggat
     1741 ttcagaaaaa aagccgggaa actccatcaa aggagatcga cagagctgaa aaattacgag
     1801 agaagtatct ggcaaaaaat ggaggttagt catgaaattt aagaagcatc ttgaaaaaaa
     1861 atatgatacc gaagcgaaaa tgtcagaatt tcttaaagga tatgaggaat tcaaactcgg
     1921 tgtaatgtta aaagaggaac ggaaaaaagc caacatgacc cagtcacagg tcgctgaaag
     1981 aatgggaacg caaaaagaag ccatttcgag aatagaaaac cattccaaaa atataaatat
     2041 tgaaacactc ttgaaatatg ccgccgcgct cggactgaaa gtaaaaataa tttttgaaag
     2101 ctgactgtgc aagaaagtca aaaaataaaa atataaaaga agtaatcttc tcaaaattaa
     2161 gataaagtct ttccagcaga ttcatcgccg aaattgatga aaaattttat gaaattctga
     2221 gataagatta caataaatac aagaaaacag caatccgatt ctgatttatt tcacaacaac
     2281 atcatcaata tagattcctt caagattgtc gattggatcg cttttaaaac ggaatcctat
     2341 ctggacggtt tctcctttgt atgatgaaag gtcgcctgaa aatttgtaat atgaagttcc
     2401 aagcttcttt gtgatctttg tccttgtgtt atcaagtgca tcaagcggtg aaggagtgtt
     2461 tgccataaga tatattccgc ttatcgcctc ccaggtgtca gtatcccttt tgataagtat
     2521 gtctgtgtag tcaactgggc tgtatccgcc gccttcaatg ttgacccacg ccatgaattc
     2581 aacaacatga gttcccgaca caggaatagt gatctgtgtg ttgagtataa ggatgtcatt
     2641 tgcattgtcg gtataattgc ctgtaagatt tgttgccgcg acattgcttc cgctgactgc
     2701 cgccgcaggt ccgttagtcg gggcacctat ctgccacaca ccggctattg tccagttagt
     2761 tccgccgtct tcaaaatctt cagaaacaat tatcgtccct gaatttcccg tattacctgt
     2821 gttaccggta tcgcccgtat ttcctgagtt tcctgtgttt cctgtgtcgc ctgtattgcc
     2881 ggtatcgccc gtatttcctg agtttcctgt gtttcctgtg tcgcctgtat tgccggtatc
     2941 gcccgtattt cctgtatttc ctgtgttgcc agtatttccg gtatcgccgg tatcgcctgc
     3001 atcaggatca taacactgtc cggttacgag gttgcattta acattcggga atgtcatgca
     3061 atcagcatct gatttgcaga gaaccccttc cgcttcttta tcggtaatgc agaaaccttc
     3121 ctctatcaaa cacctccagt tcatcgggca gtcagattct ttaagacact taactctatc
     3181 cttacagaaa tatgacagcg ttcctgtatt aaggtcttct ttagggttct cataatcgca
     3241 atattgactc ctgatacaat ctgcagtggt tttgcacgat gtcatattgt tttctaaaca
     3301 aaatcctaaa ccaatgtgac attctgaaaa atatggacag tcatcattag attcgcactg
     3361 aggattaatt tttttatttt tatttaaaac gcatcttaca aaaaaatcat tgctatggtg
     3421 agcagaacta atttgagcca tttgaaaatt tatataccat acattagaat tttggaaaaa
     3481 acttgatgac caataaggtc cgatatcttt a
//
LOCUS       DAOOYB010000293         3496 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_32164_length_3496_cov_6.893054, whole genome shotgun sequence.
ACCESSION   DAOOYB010000293 DAOOYB010000000
VERSION     DAOOYB010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3496
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32164_length_3496_cov_6.893054"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /locus_tag="PL056_12340"
     CDS             <1..864
                     /locus_tag="PL056_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004608285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HPM47853.1"
                     /translation="INNVETLANVPFVFLKGSDYFSSIGTATSKGTKVFALSGRVERT
                     GLVEVTMGTTVREIVFSIGGGIPNERKYKAAQIGGPSGGCISDINLDIEIDYERLKTV
                     GAMMGSGGLVVMDDRACMVDVAKFFMDFIQRESCGKCIPCREGTKRMLETLEMITRSR
                     TKETKEQALVRFQSVMQLTELAEVIKSTSLCGLGQTAPNPVLSTLKWFRDEYDAHIYD
                     RKCPAGACKDLVTYKIIEDKCKGCTICAKKCPVTAIKGAPKSPHYIIEDKCIKCGACL
                     AACPFGAIITE"
     gene            876..2597
                     /locus_tag="PL056_12345"
     CDS             876..2597
                     /locus_tag="PL056_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HPM47854.1"
                     /translation="MNTMTVNGKEVPFNGNETILTALKREGIKIPTLCHIEGLPPTGA
                     CRICVVEDAKTGVLIPSCSFPATDKMSILTHSPKVIKARKTIVELLLANHPDDCLYCD
                     RNLNCALQTLAEEHGVRQRRISAEKIKQFVDNSSPSIMRDPEKCILCGKCVRTCEEIQ
                     GVSALDFSGRGSKSRISTAFNVSLNLSTCVACGQCVNACPTGALVERSSIREVVNALQ
                     DPEKIVVIQHAPAVSVSLAEELGLEPGTDIIGVMTSALKQIGFNYVFDTSFSADLTIM
                     EEATELVHRITEKGALPMITSCSPAWIKFAETFYPELLPNVSSCKSPQQMLGSMIKNY
                     WAPKMNIPSEKIFSVSVMPCTAKKFEIEREEMVSKGISDIDAVLTTRELAKLIRMYSI
                     DIRKAGSAECDLPFGERSSAGKLFGASGGVMEAAIRTAYWMITGNELTNLVISELRDK
                     ENYREITVDINGLKVKAAVVSGLGNARLVLDKMMKKECDLHFLEIMSCPGGCINGGGQ
                     PRAIDKEAVKKRLASLYSIDSTAEIRVSHKNSSVMDLYEKFLGKPGSHKAHELLHTTY
                     QKRTDLI"
     gene            2609..3004
                     /locus_tag="PL056_12350"
     CDS             2609..3004
                     /locus_tag="PL056_12350"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPM47855.1"
                     /translation="MEELRNRIMLVDDDPDFLEPIKMALESHSYIVETANSEEGAVKL
                     MDVFKPDLAVFDLMMDNYDSGFILSHKLKSRYPDALVIIATGVTKETGFRFSADSKYG
                     GNWTNADLIMDKGIRPDQILKEIKKLLKS"
     gene            3015..>3496
                     /locus_tag="PL056_12355"
     CDS             3015..>3496
                     /locus_tag="PL056_12355"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPM47856.1"
                     /translation="MNYLKLLIVDDDQRMRESCSMLLETLKIKLPWLEDEYRFQITET
                     GTAEEAKSLIESQKTDILLLDQHLPGIQGTDLLEYISSQEIDIQTVMITGNATLDTAV
                     SAARNGANEILAKPFTPEELRTAVTKAAKFLILQRIVSKLDEERKKTRFEFIRVLSHE
                     L"
BASE COUNT         1117 a          733 c          843 g          803 t
ORIGIN      
        1 ataaacaatg ttgaaaccct ggctaatgtt ccatttgtct tcctcaaggg atcggactac
       61 ttcagttcga taggaactgc aacaagcaaa gggacgaaag ttttcgctct gagcggaaga
      121 gttgaacgga ccggtcttgt tgaagtaacg atggggacaa cggtaagaga aatagttttc
      181 agtatcggcg gaggtatccc gaatgaaaga aaatacaagg cggcacagat cggaggtccg
      241 agcggcggat gtatttccga tatcaatctt gacatagaaa tagactatga acggctgaaa
      301 actgtcggtg caatgatggg atcaggcggt ctggtcgtaa tggatgacag ggcatgcatg
      361 gtcgatgtcg caaaattctt catggatttc atacagagag agtcatgcgg aaagtgcatt
      421 ccgtgccgcg aagggacaaa aaggatgctc gaaacacttg aaatgataac ccgctcaagg
      481 acaaaagaga caaaagaaca ggcacttgtc agattccaga gcgtaatgca gcttacagag
      541 cttgccgaag tgataaaatc gacaagtttg tgcgggctgg gacagactgc gccgaaccct
      601 gttctttcaa cactgaaatg gttcagggac gaatatgatg cccatattta tgacagaaaa
      661 tgtcctgccg gagcatgcaa ggatcttgtg acatataaaa taattgaaga caaatgcaag
      721 ggctgcacta tttgtgcaaa gaaatgtccc gtaacagcaa taaaaggagc tcccaagtca
      781 ccccactaca tcatagaaga caagtgtatc aagtgcggtg cctgccttgc ggcatgtcct
      841 ttcggcgcaa ttataacaga atgatggaga gagctatgaa tacaatgact gttaatggaa
      901 aagaggttcc tttcaacggg aacgaaacga tactgaccgc acttaaaaga gaaggaataa
      961 agataccgac gctctgccac attgaagggc ttccccctac cggtgcgtgc agaatatgtg
     1021 tcgtggaaga tgcaaagacc ggagtgctta tcccttcatg cagctttccg gcaactgata
     1081 agatgtcaat acttacacac agtcctaaag taataaaggc aagaaaaacc atcgtggaac
     1141 tgcttcttgc aaaccatcct gatgactgtc tctactgcga cagaaacctc aactgtgcat
     1201 tgcagacact tgccgaagag cacggtgtca ggcagagacg aatcagcgca gaaaagataa
     1261 aacagttcgt tgacaactca agtccttcaa taatgcgtga ccctgaaaag tgcatactct
     1321 gcggaaaatg tgtaagaacc tgtgaagaaa ttcagggagt ttcggcactt gatttcagcg
     1381 gaaggggatc aaaaagcaga atatccacag ctttcaatgt atcgctgaac ctttcaacct
     1441 gtgttgcctg cggacagtgt gtcaatgcct gcccgacagg ggctcttgtt gaaagatcaa
     1501 gtatcagaga agtcgtcaac gcacttcagg acccggaaaa aatcgttgtc atccagcatg
     1561 ctccggcagt cagtgtctcc cttgccgagg aactcggtct tgagccgggc actgacatta
     1621 tcggagttat gacatcagct ctcaaacaga tcgggttcaa ctatgttttt gacacttctt
     1681 tctctgctga ccttacgatt atggaagaag cgaccgagct tgttcacagg ataacggaaa
     1741 agggtgccct tccgatgata acatcctgct ctccggcatg gataaagttt gctgaaacat
     1801 tctatcctga gcttcttccg aatgtatcat catgcaaaag ccctcagcag atgctcggtt
     1861 ccatgataaa gaattactgg gctcccaaaa tgaatatccc ttctgaaaag atattttcgg
     1921 tcagtgtcat gccatgcacc gcaaagaaat tcgagatcga acgggaagag atggtttcaa
     1981 aaggaatatc cgatattgat gcggtcctta caacaagaga gcttgccaaa cttatcagaa
     2041 tgtattcgat cgacatcaga aaggcgggat ctgctgagtg tgaccttcct ttcggcgaaa
     2101 gatcatcagc aggaaagctt ttcggagcaa gcggcggagt aatggaagct gctataagaa
     2161 cagcttactg gatgataaca ggaaatgaac taacaaacct tgtcatatca gagcttcgtg
     2221 acaaggaaaa ctacagagag ataacagttg acataaacgg tctgaaagta aaagcggcag
     2281 ttgtcagcgg tcttggaaat gcgcggcttg tgcttgataa aatgatgaaa aaagagtgcg
     2341 atcttcactt tcttgagatc atgtcctgcc ccggcggctg tataaacggc ggaggtcagc
     2401 caagagcgat cgataaagaa gcggttaaaa aaagacttgc ctccctttat tcaattgaca
     2461 gtacagctga aataagggtg agccataaga acagttcagt aatggacctt tatgaaaagt
     2521 tccttggaaa accgggaagc cataaagctc acgaactgct tcatacaaca taccagaaaa
     2581 gaacagatct aatctaaggg ggaataaaat ggaagaactt cgcaacagga taatgctggt
     2641 cgacgacgac cccgactttc ttgagccgat aaagatggcg ctcgaaagtc acagctacat
     2701 tgtggaaacg gcaaacagtg aagagggtgc cgtaaaactg atggatgttt tcaagcccga
     2761 tcttgcagtt ttcgatctta tgatggacaa ctacgattca gggttcattc tcagccacaa
     2821 gcttaaatcc agatatcccg atgctcttgt gataatagca acaggtgtca caaaagagac
     2881 aggtttcaga ttttcagcag attccaaata cggcgggaac tggacaaatg ctgatcttat
     2941 aatggacaaa gggatccgtc ctgaccagat attaaaggaa ataaagaagc ttttgaaaag
     3001 ctgaggagga tacgatgaat tatctcaagc tgctgatagt tgatgatgac cagagaatgc
     3061 gcgaaagctg ttcgatgctt cttgaaacat tgaagatcaa acttccatgg ctcgaagatg
     3121 aataccgttt tcagataact gagaccggaa cagccgagga agcaaagagt ttaattgagt
     3181 cgcaaaaaac agacattctc cttcttgatc agcatcttcc cggcattcag ggaacagatc
     3241 tgcttgagta tatttcaagt caggaaattg acatacagac tgtcatgata accggaaatg
     3301 cgacacttga tacagcagta agtgcagcac ggaacggtgc aaacgagata ctggcaaaac
     3361 ctttcacacc tgaagagctc agaactgccg ttacaaaagc ggcaaagttc ctgatactcc
     3421 agaggatcgt ttcaaaactc gatgaagagc ggaaaaagac aagatttgaa ttcatcagag
     3481 ttctttcgca cgaact
//
LOCUS       DAOOYB010000294         3484 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_32317_length_3484_cov_9.904345, whole genome shotgun sequence.
ACCESSION   DAOOYB010000294 DAOOYB010000000
VERSION     DAOOYB010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3484
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32317_length_3484_cov_9.904345"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="PL056_12360"
     CDS             complement(<1..392)
                     /locus_tag="PL056_12360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47857.1"
                     /translation="MRNTLIILLLFLFCSCTSCNGENSSDIDHESVIDKDSNTATDLD
                     VETNDTIDEISDDDAAITDPDVLDSDSIKQDKDDVADKDTQPDESNDIELPDYDYPAG
                     TDGDPDCPSLLNAGFPYKDGDGKITFCR"
     gene            complement(617..2233)
                     /locus_tag="PL056_12365"
     CDS             complement(617..2233)
                     /locus_tag="PL056_12365"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HPM47858.1"
                     /translation="MYRLFFLTAFFVVFLTGCGKDITLVHVNDTHSHLFGSEVHCRYK
                     GDSYNFTSGGLDLILQSVKDIRKDKSNVMFLHAGDLIQGTRYFREFQGAADVEIMNEA
                     HLDALSLGNHEFDKGSEFLHDLLVKAQFPVVAANIEMDDDVHLKKIVKSYVVTGTWPF
                     RNGVIGVVTPTTGEISSPGQGVRFTDPAEAVRKNIAELKKRRITRIFVLSHLGFEEDI
                     KLAQNVEDIDIIIGGHSHTMLGYTGIDCLNVSGDYPHIVKDPSGRNVLVVQAWDHARA
                     FGALDIEFDLKGEIQNYNGKTVYPLRKEEKIPFQMMLSPFNEFSYVNVDEDIKEKFDN
                     YEESVSKEFSRKIGSISETLEHCWEKGSDIAPLVAEAILWKMNLENRSVDVALQNAGG
                     VRKTLKKGDVTFGEVADTLPFFNNVMLFKISGADLVSALKNSVDNVFSGIHLGSFPYL
                     AGMEFEITDRNVDNFKIIENGIPKPVDPERIYTVATDSYIAQGGNGYEIFGNIKEKEM
                     TPFIVSDIFSEYIKEKKEICKPAKKHPAAE"
     gene            complement(2241..>3484)
                     /locus_tag="PL056_12370"
     CDS             complement(2241..>3484)
                     /locus_tag="PL056_12370"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HPM47859.1"
                     /translation="GDTGNTGNTGDTGDTGDTGDTGDTGDTGDTGAPCGSVKFDGTSG
                     YVEVIHNSVLNLSGNWTLEAWVMQYSTDGQMPIIRKGNTTTNPAYYIYGYEYQIFFPS
                     NAYGGYYYITDTSESVTANTGFDINKWYHIALVKNGTELKIFTNGVADGTETVSNNPM
                     NNTENLFFGSRRSSTPSYLDGLIDEIRISSTARYSSNFTPDKRLNNDSNTIGLWHFEE
                     GSGTVADNLAGSGLDGTLKGGATWTNQCVDNYEVPVVEKVDTIGSSDTDYYGTSRMRG
                     NFYSVTKSKTLTKIEAYLSLPDAGATIYYDIYESATLDGTYTHKISYTVPFTLGGASF
                     YSSGAIWVDLEAGKFYYIGSRWQSESVTYYYNANSGQSYLTDFGKWEMGMINGVGTYP
                     STFTYNDTYIDYAYYQRLTTK"
BASE COUNT          960 a          766 c          631 g         1127 t
ORIGIN      
        1 tttctgcaga atgttatttt accatcaccg tctttatacg ggaatcctgc attcaacaga
       61 ctcggacagt cgggatctcc atcggttcca gcaggataat cataatcagg aagctcaatg
      121 tcgttagatt catcaggttg ggtgtctttg tcagcaacat catccttgtc ctgttttatg
      181 gaatcactat caagtacatc tggatcggtt attgcagcat catcatcaga aatttcatca
      241 atagtgtcgt ttgtttcaac atctaaatca gtagcagtat tcgaatcttt atctattact
      301 gattcgtggt ctatgtctga actgttctct ccgttgcagg atgtacaaga gcagaatagg
      361 aataaaagca atataattaa tgtgttacgc attaaatcct ccccaccctg agtttatcga
      421 agggcaaaca ataagggcgg atacggtgat ccgcccctac aaaaaaacct accatcttgt
      481 ctcttgaaat atcatcccgc taaaacagga aaagcaagct ttatttgaat tttttgtcat
      541 ttttgcttcc aaccgaaatc tgatcaattt gtattaaaat attttcagca agtcaaggga
      601 aaaatttaga gatttactat tccgccgcag ggtgtttttt tgcgggtttg cagatctctt
      661 ttttttcttt gatatattca gagaaaatgt cgctgacgat gaagggggtc atttcttttt
      721 ccttaatatt tccgaaaatt tcgtatccgt tgcccccttg agcgatataa gaatctgttg
      781 caactgtata aattctttca ggatctacgg gtttagggat cccgttttca attattttaa
      841 aattatcaac atttctgtca gttatctcaa actccattcc ggcaagatat ggaaatgagc
      901 cgagatgaat tcctgaaaac acattgtcaa cactgttctt gagcgcactg acaagatctg
      961 caccgcttat tttaaagagc atcacattgt taaaaaatgg taatgtgtca gcgacctctc
     1021 cgaatgtaac atcccctttt ttaagggttt ttctcactcc gccggcattc tggagagcaa
     1081 catcgaccga tctgttctcc aaattcattt tccaaagtat cgcctctgca acaaggggcg
     1141 ctatgtcact tcctttttcc cagcagtgtt caagagtttc tgaaatactt cctatctttc
     1201 tgctgaactc ttttgaaaca gattcttcat agttgtcgaa tttttcctta atatcttcat
     1261 caacattgac atatgaaaac tcgttgaaag gcgaaagcat catctgaaaa ggaatttttt
     1321 cttcctttct caaaggatag accgttttcc cgttgtaatt ttgtatctcc cctttcaggt
     1381 caaactcaat atcaagagct ccgaaagccc ttgcatggtc ccatgcctga acaacaagaa
     1441 catttctacc tgacggatct ttcacaatat gaggataatc gccgctcaca ttaaggcagt
     1501 caattccggt atatccgagc attgtgtgag aatgtccacc gataataata tcaatatctt
     1561 caacattttg tgcaagtttt atatcttcct caaaaccgag atggcttaga acaaaaatcc
     1621 tggttattct cctttttttg agttctgcaa tattttttct taccgcttca gcaggatcgg
     1681 taaatctcac cccctgcccg ggtgaagaaa tttcaccggt tgtcggagtt acaacaccta
     1741 tcacaccgtt tctgaaaggc catgtgccgg tcacaacata agattttaca atttttttca
     1801 gatgaacatc atcatccatt tcaatgtttg cggcaacaac cggaaactga gctttcacaa
     1861 gaaggtcgtg tagaaattca cttcctttat caaattcgtg attgcccagg gaaagagcat
     1921 caagatgggc ttcgttcata atttccacat ctgcagcacc ttgaaattcc ctgaaatatc
     1981 ttgttccctg aatgagatct ccggcatgaa ggaacataac attgctttta tcttttctta
     2041 tatctttaac cgattgcaga atcagatcaa gtccgcccga agtgaaattg taactgtcac
     2101 ctttatatct gcagtggact tcacttccga aaaggtgtga atgggtatcg ttgacatgaa
     2161 caagggtgat atcctttccg catcctgtaa gaaaaacaac aaaaaacgcc gtcagaaaaa
     2221 acagtctgta catattttcc ttattttgtc gtaagtcgct gataataagc ataatcaata
     2281 taagtgtcgt tataggtaaa tgtagaagga tatgtcccga ctccgtttat cattcccatt
     2341 tcccattttc caaaatcggt taagtagctc tgtcctgaat ttgcgttata gtaatatgta
     2401 acagactcag actgccatct tgaaccgatg taataaaatt tgcctgcttc aaggtccacc
     2461 catatagctc ccgaactgta aaatgatgct ccgcccagag taaacggcac tgtatatgaa
     2521 atcttgtggg tatatgttcc atcaagagtc gcagattcat aaatatcata atatattgtc
     2581 gctcccgcat cgggcagact cagatatgct tctatttttg ttaatgtttt agatttggtg
     2641 acagaataga aattgcctct cattcttgaa gttccgtaat aatctgtgtc gcttgaaccg
     2701 atggtatcca ccttttcaac aaccggaact tcatagttgt caacacactg attcgtccat
     2761 gtagctccac ctttcagagt gccgtccagc cctgatcctg ccaaattgtc cgcaacagtt
     2821 ccggatcctt cctcaaaatg ccagagccct atggtatttg aatcattgtt cagtctctta
     2881 tccggagtga aatttgaaga ataacgggct gttgaagaaa ttcttatttc atctataaga
     2941 ccatctaaat atgaaggagt gcttgatctt cttgagccga agaacaggtt ttcagtgttg
     3001 ttcatcgggt tgtttgaaac cgtttcagtt ccgtctgcga caccgtttgt gaaaattttc
     3061 aattcagtgc cgttttttac aagtgcaata tgataccatt tgttgatgtc aaaaccggtg
     3121 tttgctgtaa cagattccga agtatcagta atataataat atccgccgta tgcgttactt
     3181 ggaaaaaaga tctgatattc atatccgtaa atgtaatatg ccggatttgt tgtagtattc
     3241 ccttttctta ttatcggcat ctgaccgtct gtgctgtact gcataaccca cgcttccagt
     3301 gtccagtttc ctgacaaatt aagcactgaa ttgtggatga cttcaacata gccgctcgtt
     3361 ccgtcaaatt ttactgaacc gcagggagca cctgtatctc ctgtatctcc tgtatctcct
     3421 gtatctcctg tatctcctgt atctcctgtg tcaccagtgt tgcctgtgtt gcctgtatcg
     3481 ccag
//
LOCUS       DAOOYB010000295         3478 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_32387_length_3478_cov_12.563249, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000295 DAOOYB010000000
VERSION     DAOOYB010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3478
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32387_length_3478_cov_12.563249"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="PL056_12375"
     CDS             complement(<1..609)
                     /locus_tag="PL056_12375"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPM47860.1"
                     /translation="MYLFKDVYILNPTDSESFDFFRGSLLVEGKSILKIFNGQLPESF
                     EISGDCEVVEGKGTLMIFPGFIQTHIHLCQTLYRNRAENMALLPWLKEHIWPYEAKLT
                     KKTIGQSVILSLKEILSSGTTAVLDMGTVRHTDVIFEIMEIIGFRYTGGKAMMDAGEG
                     VPADLAEKTDDSINESVRLYEKFHNKNNGMLKYCFAPRFVLSC"
     gene            709..1851
                     /locus_tag="PL056_12380"
     CDS             709..1851
                     /locus_tag="PL056_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47861.1"
                     /translation="MIFVGSPFFYTTLLVCGIFIILRKKIKSPVFKIVWINIVVILFF
                     FSLFDTVAVKMFSNINPFRILDNRFHHSLKPFTNEFTTWDTEGKFNYLIQTNSLGFVD
                     SETRTVNMKKKGKRIMFIGDSFIEGVGYPWEETVAGRISEKLKSEGVELLNASVASYS
                     PKLYWLKTEYFLQAGLEIDELFVFVDVSDIIDEVVYDYFVPETFSKTRRIMEPVIDFF
                     SKNSFIYRSYRIRYVIEQDNPYRESSEFWGGIKNFYLLKPQWIFNEQAFEMYGRIGIE
                     LAEKHMDKLHKLCKKNKIKLTIVVWPWDINLKKKHDRKHLDIWKNFASKRNIDLIQMY
                     DLFESIPIETIDRYFIPEDIHWSKKGNFMVSEYLWEQMNKNKGEKK"
     gene            1848..2879
                     /locus_tag="PL056_12385"
     CDS             1848..2879
                     /locus_tag="PL056_12385"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47862.1"
                     /translation="MNLQKKIDGKKIVANIEKLVTIPSPTGYTGDVKDFLVSFAKKHS
                     IDHEVTKKGAVIFKFGPAKKEGTVFAAHIDTLGGMVNSVSDNRVKITTVGGFPALYII
                     GEICTVHSRTGKKYVGTFLPDDPAIHVNSKLKDLKPDFDNCSLRMDVELKKDEKLSDI
                     IEVGDFVSFDPGFRFINGYVNSRHLDDKASAGIFMHIAEILNLNEKNLKKRVSFFFNI
                     TEETGQGIAGTQKDDDLIVVDMGVVGKEPAGSEKCVSICAKDSSGPYNYDLTTELIDI
                     AKKNNISYKIDVFPYYGSDGSALLRSCSDSRVALIGQGVDASHGYERTHVEGLTATAS
                     LVLAYIFKG"
     gene            complement(2894..3178)
                     /locus_tag="PL056_12390"
     CDS             complement(2894..3178)
                     /locus_tag="PL056_12390"
                     /inference="COORDINATES: protein motif:HMM:NF021873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HPM47863.1"
                     /translation="MEGINHPRIIDLEILEDFEIIAVFSDGSVKRFNFKPLLKEGVYK
                     DLSNPSFFKMAKIDAGGYGISWSDLIDIDSNDIWVLGEPAPEYNSKKKDQ"
     gene            complement(3184..3279)
                     /locus_tag="PL056_12395"
                     /pseudo
     CDS             complement(3184..3279)
                     /locus_tag="PL056_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008722843.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
BASE COUNT         1130 a          676 c          665 g         1007 t
ORIGIN      
        1 gcatgaaagg acaaatctcg gagcaaagca atatttaagc atgccgttgt ttttattatg
       61 gaatttctca taaagccgta cactttcgtt tattgagtca tctgtttttt cagcaagatc
      121 ggcgggaact ccttcccctg catccatcat cgccttccct ccagtataac ggaagccgat
      181 aatctccata atttcaaata ttacatcggt gtggcggact gtccccatat caagaacggc
      241 tgtcgttcct gacgaaagga tctcctttaa cgacagtata acgctctgac caatagtttt
      301 ttttgtgagt tttgcctcat acggccagat gtgctctttc agccacggaa gaagagccat
      361 gttctccgct ctgtttctgt aaagtgtctg gcacagatga atgtgggtct gaataaaacc
      421 gggaaaaatc atcagtgttc ccttcccttc aacaacttca caatcgcctg atatttcaaa
      481 gctttcaggc agttggccat taaatatctt taaaatgctt ttcccctcga caagaagcga
      541 acccctgaag aaatcaaaac tctccgaatc cgtcggattc agaatgtaaa catctttgaa
      601 taaatacaaa accctctcct gaaaaaaaat tcgtaacatt ataaaacaaa aagtaccgtc
      661 cttacaaata tttctggaat tttttatcat atctgttata aattcagcat gatttttgta
      721 ggcagtcctt tcttttacac aaccctgctt gtctgcggga tatttatcat tctaagaaaa
      781 aaaattaaat ctcccgtatt taagatagta tggataaaca tcgttgtaat tctgtttttt
      841 ttctctcttt tcgatactgt tgctgtaaaa atgttttcca atatcaatcc gttcagaatt
      901 ctcgacaacc gctttcacca ttctttaaaa ccatttacaa atgaatttac cacatgggat
      961 acggaaggaa aattcaatta tctgattcag accaactctc tcgggtttgt tgacagtgaa
     1021 acaagaactg taaacatgaa aaagaaagga aaacgaatta tgttcatcgg cgacagtttt
     1081 atagaaggag ttggttatcc ctgggaagag actgttgcag gaagaatctc tgaaaaactt
     1141 aaaagtgaag gggttgagct tctaaacgct tctgtcgcaa gctattcacc caagctatac
     1201 tggctgaaaa ctgaatattt tcttcaagcc ggacttgaga tcgacgagct ctttgttttt
     1261 gttgatgtgt ctgatatcat tgatgaagtc gtctatgatt attttgttcc ggagaccttt
     1321 tcaaagacca ggcggatcat ggagccggtt attgatttct tttcaaaaaa ctcatttatt
     1381 tacagaagtt acaggatcag atatgtgatc gaacaggaca atccttaccg tgaaagttca
     1441 gagttctggg ggggaataaa gaatttctac cttctgaagc cgcagtggat atttaatgaa
     1501 caggcctttg aaatgtatgg tagaattggg attgagcttg cagaaaaaca catggataaa
     1561 ctgcataaac tctgcaaaaa aaataagata aagcttacga tcgtcgtttg gccgtgggat
     1621 atcaatttaa agaaaaaaca tgacaggaaa catcttgata tctggaagaa ttttgcttca
     1681 aaaaggaata tcgatcttat tcagatgtac gatctttttg agtcgatccc gatagaaacg
     1741 attgaccgtt atttcattcc agaggacatt cactggtcaa agaaaggaaa ttttatggtt
     1801 tctgaatatc tctgggaaca gatgaacaaa aataaaggag aaaagaaatg aaccttcaaa
     1861 aaaagatcga cggtaaaaag atcgttgcca acattgaaaa acttgtcacc atcccttcac
     1921 ctaccggcta caccggagat gtaaaggatt ttcttgtctc ttttgcaaaa aaacactcta
     1981 tagatcatga agttacaaaa aaaggggctg taattttcaa gttcggacct gcaaagaaag
     2041 agggaactgt atttgccgct cacattgaca ctctcggagg aatggtgaac tcagtttcag
     2101 ataaccgggt gaagatcact actgtcggag gatttcccgc gctctacata attggcgaaa
     2161 tatgcacagt tcactctaga acaggtaaaa aatatgtcgg gactttcctt cctgatgatc
     2221 ctgcaattca tgtcaacagc aagctcaaag accttaaacc agattttgac aactgttcgc
     2281 tgagaatgga tgttgaactc aaaaaagatg aaaaactgag cgatattata gaagtcggtg
     2341 atttcgtttc atttgacccc ggattccggt ttattaacgg atatgtcaat tcaagacatc
     2401 ttgatgacaa ggcatctgcc ggaattttta tgcatattgc tgaaatcctt aatttaaatg
     2461 aaaaaaatct taaaaaacgg gtctcttttt tcttcaacat cacagaagag acaggacagg
     2521 gaattgcagg aactcagaaa gatgacgatc ttatcgttgt tgatatgggt gttgtaggca
     2581 aagaaccggc aggaagcgaa aaatgtgtca gcatctgcgc aaaggattcg tcaggcccgt
     2641 ataactatga cctcacaaca gaactcattg atattgccaa gaaaaataat atcagctaca
     2701 aaattgatgt cttcccctac tacggctcag acggatcagc actgcttaga tcatgcagtg
     2761 attcaagagt cgctcttatc ggacagggcg ttgacgcaag ccacggatac gaaagaaccc
     2821 atgtcgaagg attgacagcc accgcatcac ttgtgctggc ttatattttt aagggataga
     2881 atttcctcga tttttattga tctttctttt tagaattata ttccggagca ggttctccaa
     2941 gaacccatat atcgttagaa tcaatatcaa taagatcact ccatgaaatt ccgtaaccac
     3001 ccgcatcaat tttcgccatc ttaaaaaatg aagggtttga aagatcttta tacacgccct
     3061 ctttaagtaa cggcttaaaa ttaaatcttt taacagagcc atcgctgaaa acagcaataa
     3121 tttcaaagtc ttcaagaatt tcaaggtcta taattctggg atgatttatt ccttccatgg
     3181 caactcgccg taaattgcgt gaaaatgagg cggattgtgc tcagatttga aaaagaaaat
     3241 cttaacaaca attccataaa atctcgctat aacaggcatt tatccctcct gaaaaaacct
     3301 gtcgaattat actttataaa aattgattgt caaatgaatc actaactcgc tgaaaatcag
     3361 aaaaagcgga atttatttag gggattggga tgtatatgat gagggaagat gtgcgagata
     3421 gcatcacaaa cctttgaaca aaaaagagtt cgtggtcaat tatcgcgaac acacatta
//
LOCUS       DAOOYB010000296         3473 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_32463_length_3473_cov_8.900819, whole genome shotgun sequence.
ACCESSION   DAOOYB010000296 DAOOYB010000000
VERSION     DAOOYB010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3473
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32463_length_3473_cov_8.900819"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..1214)
                     /locus_tag="PL056_12400"
     CDS             complement(63..1214)
                     /locus_tag="PL056_12400"
                     /inference="COORDINATES: protein motif:HMM:NF020571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1887 family CARF protein"
                     /protein_id="HPM47864.1"
                     /translation="MGRKVLISIVSDQTIPNVLFIKDFKEMDEYVFLSTEKMEQQNQT
                     EKIIKSSGLDKTKCHIEKIHESDIDEIKKVLTNIPLSQDAEFIVNITGGTKIMSLAVY
                     DFFSKFKNSVIFYMPIGENGYQKIFPETENLKAINFKVSLQNYLSAYGVEIKKQSATT
                     KDLNFLNTFKTELFNNYRNEIAFLIKLQNDRPIKKVLEKKGEIDWNDIRVHEFVHKNS
                     GIYLLDNIDKLFKSCGFNDGVMIKSKSLRFITGGWFEELAYSLVKENLKISDEQIALG
                     TEVIKDNDNRKKNEFDVAFISNNSLYIIECKTSMESEVKGLFAETVYKQAAIRKDLGL
                     SAQSYLFTLDKVDNIDHIKRAETLGIKIIDLTVLNDSGKLEETFFKKFK"
     gene            complement(1196..1600)
                     /gene="csx20"
                     /locus_tag="PL056_12405"
     CDS             complement(1196..1600)
                     /gene="csx20"
                     /locus_tag="PL056_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Csx20"
                     /protein_id="HPM47865.1"
                     /translation="MSNLFLLFSHTLTPEQVKDANEVLGVEKIVSLPLSLQQMWSDIN
                     PVGELNTKPLEEILLWLKEHASKGDFVLIQGEFGATHYLIEHCFNVGIIPIYATSKRI
                     YEEIRNGDGTVERKHIFKHVNFRRYVKWGEKY"
     gene            complement(1600..2634)
                     /locus_tag="PL056_12410"
     CDS             complement(1600..2634)
                     /locus_tag="PL056_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004070919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HPM47866.1"
                     /translation="MDQDFNQFISEVKERIRSAQYAAMRAVNSEMTALYWDVGKMIVE
                     KQETLGWGKAVVEQLSLELQKDFPSAKGFSTQNLWNMRQFFLYYRENENLQPLVGEIS
                     WTKNILILQRCSDNFQKEFYLRMTAKFGWTKNVLIHQIESGAYEKYLLNQTNFDQTVP
                     EKYRNQAILAVKDEYSFDFIGGSQEHSEKELEYELMKNIRSFLLEMGNDFCFIGNQFR
                     ISTETKDYYIDLLLFHRKLKSLIAVELKIGEFIPEYAGKMQFYLALLDDFVKNSDENP
                     SIGIIICKNKDRTEVEYALRLTDAPIGVATYKITKTLPASMKNILPGPEIIAERILKL
                     KEITEKKEIP"
     gene            complement(2634..>3473)
                     /gene="csm5"
                     /locus_tag="PL056_12415"
     CDS             complement(2634..>3473)
                     /gene="csm5"
                     /locus_tag="PL056_12415"
                     /inference="COORDINATES: protein motif:HMM:TIGR01899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated RAMP protein Csm5"
                     /protein_id="HPM47867.1"
                     /translation="NRELFSKISEKKSIPNSISGQNIKKHIKTNGKIYLPGSSIKGAI
                     RTAIAASYAYDENHNYQDLLSSINDYCRDTKYADKKLLESMFGKDPKENLMRILTVTD
                     ATVGKSSRIYEMLVLNSVRMRGKKIFYEMIPENTELRCSITLDQFLSDEENRKKAKIP
                     KHAFSFNEIIEAIKEDADANAVINMDFQNNEISSFYKMLKENYLDKGDIVFNLGAGIG
                     WIGTTGNLIGKKLTESVRKNLRLSSDADHIKQPFPKTRRVVDVGRKIVPPGWVKMTFE
                     EIL"
BASE COUNT         1033 a          661 c          510 g         1269 t
ORIGIN      
        1 ccgcttttaa caagcctgcc ccagtctgaa ataatgtaaa ttgtggacat ttttagttcc
       61 ctttatttaa actttttaaa gaaagtctct tccaattttc cagaatcgtt caaaaccgta
      121 agatcaatga tctttatccc cagagtttct gctctcttta tatgatcaat gttgtcaact
      181 ttatcaagcg tgaacaaata actttgagca gaaagtccaa gatcttttct gatagcggcc
      241 tgtttataga cagtttcagc aaaaagtcct ttaacttcag actccatgct ggttttacat
      301 tcgatgatat acaaagagtt gtttgatata aatgccacat cgaattcgtt tttctttctg
      361 ttgtcattgt cttttatgac ttctgtgcca agagcaatct gctcatctga tattttcaga
      421 ttttctttaa ccagagaata agcaagttct tcaaaccatc cgccagtgat aaatctcagg
      481 gatttagatt taatcatgac tccatcatta aacccgcatg atttgaaaag tttatcaatg
      541 ttgtcaagaa gatatatgcc gctgttttta tgaacaaatt catgaactct tatatcgttc
      601 caatctatct cccctttttt ctccagaact tttttaatag gtctatcatt ttgaagtttt
      661 ataaggaatg caatttcatt tctataatta ttaaaaagtt ccgtcttgaa tgtattaaga
      721 aagttcagat ccttcgtcgt tgctgattgt ttttttattt caacaccgta agcagaaaga
      781 taattctgaa gtgaaacttt aaaattgatc gcttttagat tttcagtttc agggaatatt
      841 ttctgataac cattttcgcc aataggcata taaaaaataa ctgagttttt aaatttgctg
      901 aaaaaatcat aaaccgcaag tgacataatc tttgttccgc cagtaatatt aacaataaat
      961 tcagcatctt gggacaaagg aatgtttgtg agtacttttt tgatctcatc aatatcgctt
     1021 tcatgaattt tttcaatatg gcattttgtt ttatccaaac ccgatgattt tattattttt
     1081 tctgtctgat tctgttgttc cattttttca gttgacagaa aaacatattc atccatttcc
     1141 ttaaaatctt taatgaaaag cacattcggt atagtctggt cgctaacaat tgaaattaat
     1201 acttttctcc ccatttcaca tacctcctaa aattcacatg tttaaatata tgtttccgtt
     1261 caactgtgcc atctccattc cttatctctt catatatcct tttgcttgtg gcatatatcg
     1321 gaataatccc tacattaaaa cagtgctcaa ttaaatagtg tgttgccccg aattcaccct
     1381 gaatgagaac aaaatctcct ttgcttgcat gttccttaag ccacaacaaa atttcttcaa
     1441 gtggtttggt gttcaactct ccaaccggat taatgtctga ccacatttgc tgtagagata
     1501 gaggcagaga aacaattttt tcaaccccca gaacttcatt tgcatctttt acctgttcag
     1561 gagttaaagt gtgtgagaag agaaggaata ggttagacat tagggaatct cctttttttc
     1621 ggtaatttct tttaatttta atattctttc tgcaatgatt tcgggaccag ggagaatgtt
     1681 tttcattgaa gcaggtaagg tttttgtaat tttataagtt gcaactccta ttggtgcatc
     1741 tgtcagtcgc aaagcatatt caacttctgt tctgtcttta tttttacaga taattatgcc
     1801 aatggatgga ttttcatcac tattttttac gaagtcgtca agaagagcaa gataaaactg
     1861 cattttacct gcatattcag gaataaactc accaattttc aattcaacag caataagact
     1921 tttcagtttt cgatgaaaaa ggagcaaatc aatgtaataa tcttttgttt ctgttgaaat
     1981 tctgaactga tttccaataa agcagaaatc attacccatt tcaagaagaa aactgcgaat
     2041 attcttcatc aattcatatt ccagttcttt ctcgctatgt tcctgagaac caccaatgaa
     2101 atcaaaagag tactcatctt ttacagcaag aattgcctga ttcctatatt tttcaggcac
     2161 agtttgatcg aagtttgtct ggtttaaaag atatttttcg taagcaccgc tttcaatctg
     2221 atgaataagc acattcttgg tccatccgaa ttttgcagtc atccgaagat aaaattcttt
     2281 ttgaaaattg tcgctgcacc tttgaagaat caggatgttt tttgtccagc taatttctcc
     2341 aaccaatggc tggagatttt cattttctct gtaataaaga aaaaactgtc tcatattcca
     2401 gagattctgt gtggagaaac ctttagccga aggaaaatct ttctgtaatt ccaaagacag
     2461 ttgctcaaca acagcttttc cccaaccaag tgtctcctgc ttttcaacga tcattttccc
     2521 aacatcccag taaagagcag tcatttcact gtttacagct cgcattgctg cgtattgagc
     2581 agaacgaatc cgttccttca cctcagaaat gaattgattg aaatcttgat ccattataga
     2641 atctcctcga aagtcatttt cacccatcct ggcggaacta tttttctgcc aacatcaaca
     2701 actcttctgg ttttgggaaa aggttgttta atgtggtcgg catcagaaga caatcttaga
     2761 ttttttctaa ctgattccgt cagtttttta cctattagat ttcctgttgt tccaatccag
     2821 ccaattccgg caccaagatt gaaaactata tcacctttat ctaaatagtt ttctttcaac
     2881 attttataaa aagatgatat ttcattgttc tgaaaatcca tattaattac agcattcgcg
     2941 tcagcgtcct cttttattgc ttcaataatc tcattaaaag aaaaagcatg tttaggtatt
     3001 ttcgcttttt ttctgttttc ttcatctgat aaaaactgat ccaatgtaat cgagcatcta
     3061 agttcagtgt tttcaggaat catttcataa aaaatctttt ttcctctcat cctgacacta
     3121 ttcaaaacca acatttcata aattcttgag cttttcccaa cagttgcatc tgtaactgtt
     3181 aaaattctca taagattttc ctttggatct ttcccgaaca tactctctaa aagttttttg
     3241 tcagcatatt tagtatctct gcaataatca tttatagaac ttaataaatc ttggtaatta
     3301 tgattttcat cataggcata agatgccgca atcgcagttc taattgcacc ttttatcgaa
     3361 gaaccaggaa gataaatttt gccatttgtt ttaatgtgtt ttttgatatt ctgtccagaa
     3421 attgagttag gaattgattt tttctcagaa atttttgaaa aaagttctct gtt
//
LOCUS       DAOOYB010000297         3463 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_32585_length_3463_cov_6.913732, whole genome shotgun sequence.
ACCESSION   DAOOYB010000297 DAOOYB010000000
VERSION     DAOOYB010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3463
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32585_length_3463_cov_6.913732"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..529)
                     /locus_tag="PL056_12420"
     CDS             complement(146..529)
                     /locus_tag="PL056_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012025640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HPM47868.1"
                     /translation="MKVLLSIKPEFAEKIFEGTKKYEFRRTIFKNPDVKTVVVYASSP
                     VQKVIGEFEVDCILTDDLEKIWQETEKFAGIDKSYFMSYFNKKEHAYAIKVKKTKRYK
                     KLLNLKESFGINPPQSFAYIEEANS"
     gene            complement(507..2006)
                     /locus_tag="PL056_12425"
     CDS             complement(507..2006)
                     /locus_tag="PL056_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015261344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HPM47869.1"
                     /translation="MKALLDTNIIIHREANTVVNKDIGSLFKWLDKGKYEKCVHPITI
                     EEIKKNPNPKTVETILAKIDSYILLKTVAPILPEVAEISDHYDKGKNDVNDSALLNEV
                     LSDRVDILITEDKRMHFKARLLNVSDKVFNIDSFLEKVVSEHPELIDYRVLQVRKKHF
                     GEIDVKDSFFDSFREDYPGFEKWFNRKADEIAYITEKDDKILSFLYLKTEDRDENYSD
                     VSPVFLPKKRLKIGTFKVVSNGLRLGERFLKIIFDNAIINRVDEIYVTIFEKRMDQAR
                     LINLLEDWGFMKYGKKGEELVYVRDFTPNYDTENPKLTFPYFSKNANVFLNPIYPEYH
                     TNLLPDSILKTESPENFIESQPHRNAISKVYISRSWERNLKCGDIIIFYRTGGYHKSV
                     ISTIGIVDDVIFDIEDEADFIRKCRKRSVFKDLELSEYWNYSESNRPFIVNFLYTYSF
                     PRRINLAKMIELGIIKDIESAPRGFQKITKQQFETILKETGTDESIIVD"
     gene            complement(2033..2671)
                     /locus_tag="PL056_12430"
     CDS             complement(2033..2671)
                     /locus_tag="PL056_12430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47870.1"
                     /translation="MLKQTVYWFFFSGIVLTFMVFKDDFSFKKIIIGSFGVTVIVEFL
                     VNIYVFPFYVEVLLIPITFLFTAFIPILGNMKDSKENLQAIQMSNIILSMIGFLLLFF
                     SVFKIVSDDSFYLGETVFDFLFPVIMTVSFIPYLFLVRILTSYESVFVIGKIFFDEKS
                     KMLSFLKWQLIKNFRFDIERLKDFKPIAMREFTVDSDKADIVKIIKEFNELK"
     gene            complement(2977..3117)
                     /locus_tag="PL056_12435"
                     /pseudo
     CDS             complement(2977..3117)
                     /locus_tag="PL056_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571730.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 domain-containing protein"
BASE COUNT         1083 a          677 c          479 g         1224 t
ORIGIN      
        1 acttctgcca cttcccccag cttacattcc ttccagtcag tcattttatt ttccattttg
       61 tcactcattt tttagcaaca aaccattttg tcgctcattt ttttgaaaaa gtgatcgaca
      121 aaaacaaata ttttcttcca ctcactcatg aatttgcttc ctcgatatat gcaaatgact
      181 gtggcgggtt aattccaaaa ctttctttta aattaagaag ttttttatat cttttggttt
      241 ttttaacttt tatcgcataa gcgtgttctt ttttattgaa atagctcata aagtagcttt
      301 tatctattcc tgcaaatttt tctgtctctt gccatatttt ttcgaggtcg tccgtaagaa
      361 tacaatccac ttcgaattca ccaataacct tttgaactgg agaagaagca tatacaacca
      421 ctgttttcac atcaggattt ttaaaaatgg ttcttctgaa ttcatatttc ttcgtacctt
      481 caaatatttt ttcagcaaat tcaggcttaa tcgacaataa tactttcatc tgttcctgtc
      541 tcctttaata tcgtttcaaa ctgttgtttc gttattttct gaaaacctct cggcgcagat
      601 tcaatatctt ttattattcc gagttctatc atttttgcca gatttatcct ccttgggaac
      661 gaataagtgt ataagaaatt aacaataaat ggtctgtttg attcgctata attccaatat
      721 tcacttagct ctaagtcttt aaaaacactt ctctttcggc attttcttat aaaatcggct
      781 tcgtcttcta tgtcaaagat tacatcatca acaataccaa tcgttgaaat aacacttttg
      841 tgatatcccc ccgttctata aaaaataatt atgtcaccgc attttaaatt tctttcccac
      901 gaccgggaaa tataaacctt acttattgca tttctatgcg gctggctttc tataaaattt
      961 tcaggagact ctgtttttaa gattgagtcg ggaagaagat tcgtatgata ttccggatat
     1021 atcgggttaa gaaaaacatt agcgtttttg gagaaatacg gaaaagtcag tttggggttt
     1081 tcggtatcat aattcggtgt aaaatccctt acataaacca gttcttcacc ctttttaccg
     1141 tatttcataa aaccccagtc ttcgagcaaa ttaatcaacc ttgcctggtc cattcttttt
     1201 tcgaatattg ttacatatat ctcatcaaca cggttaatta tcgcattatc aaatattatt
     1261 ttcaggaacc tctctcctaa cctaagccca ttacttacta ccttaaaagt tccgatttta
     1321 agtcttttct taggcaagaa aacaggggaa acatcagaat aattttcatc tcgatcctct
     1381 gtttttaaat agagaaacga taatattttg tcgtctttct cagttatata tgcaatctca
     1441 tctgccttac ggttaaacca tttctcaaac ccggggtaat cttctctaaa actatcaaaa
     1501 aaagagtctt ttacatctat ttctccgaaa tgtttttttc tgacttgaag aactctataa
     1561 tcaattaatt caggatgttc tgatacaact ttttccaaga aagaatctat gttgaacacc
     1621 ttgtcagata catttagcag tctggcttta aaatgcatcc gtttatcttc tgtaattaaa
     1681 atatcgacac gatctgataa gacctcattt aaaagcgcag aatcattaac atcattttta
     1741 cctttgtcat aatggtctga tatttcagcc acttcaggga gtataggcgc gacagttttt
     1801 aaaagaatat aactatctat cttggcaaga atcgtttcaa ctgttttagg attaggattc
     1861 tttttaattt cttcaatcgt tattggatga acacattttt catattttcc cttatcaagc
     1921 catttaaaca aagatccgat gtctttgttt acaaccgtgt tcgcttcacg atgaataata
     1981 atgtttgtgt caagcaatgc cttcatttta attctcctgt tttatattat ttctatttta
     2041 actcattaaa ttctttaatt attttaacaa tatcggcttt atccgaatct actgtaaact
     2101 cccgcattgc aatcggctta aaatctttca agcgctctat gtcaaagcgg aaatttttta
     2161 ttagctgcca cttcagaaaa cttaacattt tactcttttc atcaaagaag attttaccaa
     2221 tgacaaaaac actctcataa gaagttagta tccgcaccaa aaacagataa ggtataaatg
     2281 aaaccgtcat aattactgga aataaaaaat caaaaaccgt ttcccctaaa taaaaactat
     2341 catctgaaac tattttaaaa acactgaaaa acaataataa gaaacctatc attgatagaa
     2401 taatattaga catttgtata gcctgtaagt tttctttgct gtctttcata ttccccaaaa
     2461 taggaataaa agctgtaaaa agaaaagtga tcggaatcaa cagaacttct acataaaaag
     2521 gaaaaacata aatatttaca aggaattcta cgattacagt cactccgaaa gatccaatta
     2581 tgattttctt gaaagagaag tcgtctttaa aaaccataaa agtcaatact atacctgaaa
     2641 agaaaaacca gtaaacagtt tgtttaagca ttgacgagtt ccatatcccc gacaagaaca
     2701 aaattcttac cgccagaaca ctgtatgcga agaaagaaaa gaaaagcatc ttcaactttg
     2761 ttagagtctt aaatagatca aaaaaagaat ctcttacaga ctttaacctc gcaacataaa
     2821 taaatagtac aaacatccag aaaaatgttg ccaattccct gttgttaaaa ttttctgtta
     2881 tgaaatttat gatcttctca aaaacttcca aacttcacga tcctttttta agtttcttcc
     2941 actcgcaatt cgactggctc attgtgcaac tcatattcat tttctctccg caaattcttt
     3001 ttctgcttct tcgatctgtt ttttcagttc ttctttgctt ggaagataaa gctggtaatt
     3061 ggatgcgtaa atattgctgt ctttcggcag agtaagttca acgagagaat cgctttttga
     3121 aatccgagac actgtctcgg aaattagatt ctcagaaatc ccagacaccg tctgggcatt
     3181 atgcaacatc tcgaattcat ggactatttc acatttgtcc gacactgtag gactaattgc
     3241 atttgctctg tttacagatt cggcaggcag tgcctgttga atcttttgat ttgcgtttgg
     3301 agattgtgca ggcagtgcct tcgtaatctt ttgtggagta tcctgaaaga gatagatcag
     3361 tttttcaata ttcatatttc agctccgtca aaattttcct tccactcgct catattttta
     3421 cctgtctgtt tggatcaaaa aaaagaaaat gcccgtcaag gtt
//
LOCUS       DAOOYB010000298         3460 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_32631_length_3460_cov_3.794126, whole genome shotgun sequence.
ACCESSION   DAOOYB010000298 DAOOYB010000000
VERSION     DAOOYB010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32631_length_3460_cov_3.794126"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1408
                     /locus_tag="PL056_12440"
     CDS             <1..1408
                     /locus_tag="PL056_12440"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HPM47871.1"
                     /translation="ISKDGAARIKIPPNALAEDTVITVQKMDVLKYPDGDMIASSVYN
                     FGPDGLKFDKPSTLTLTATKDKPVGIGAAVAIFRDGKWVHEGSGDPIMSGDPIMSGDP
                     IMSGDPIMSGDPIMAEVGHFTEFTLIYFDIEDETPDEVVVVIDNEHIDEEIVNEEPDE
                     EHIEIDDDYEEIVDEEPDDIEEEIIIGELALIPAGSFWMGSPVEEPGRETNEVLHYVQ
                     ISKGFYVGKFEVTQGEFQSLMGYNPSSFLTCGSDCPVETVTWHEALKYANELSKKETL
                     EECYDCSGSAPDFNCSLKTAFSKPQDCLGYRLPTESEWEYAARAGTDTAIYNGVLTHI
                     GRTPLDENLNAIGWYGGNSKATYSDAYNCSGWFTGSDKCGTQSVGSKLKNNFDLYDMS
                     GNVWEWTADFDGTYPSGTELEPSVDPWQSVSSPKRVVRGGSWNNLASLCRSAYRYYLV
                     NTDRTNYLGFRVARSVEE"
     gene            1516..1875
                     /locus_tag="PL056_12445"
     CDS             1516..1875
                     /locus_tag="PL056_12445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47872.1"
                     /translation="MTKNLILMTILAIPIMMNRRMLIVAILEIPETPEIQEIRGIREI
                     QAIVGTQEILEILEILEIQETVGSYGLKFLPEVLIHVQLTLTVKLIVGEVIVLVSSEM
                     EQIAIEFFRLKLIQRVC"
     gene            1872..>3460
                     /locus_tag="PL056_12450"
     CDS             1872..>3460
                     /locus_tag="PL056_12450"
                     /inference="COORDINATES: protein motif:HMM:NF012632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47873.1"
                     /translation="MSNKKLILISSGGNHTCVVDNENKVYCWGSNDSGQLGTGADFGW
                     NDFSNVPIEIDNSGVLKGVAIVSISSGYDHTCAVSKVGKAYCWGDNEYGQLGDGTETD
                     KPTPVLVNDKGVLNGKFLLQISAGGILTCTLDDEGKAYCWGGGQYGGLGDGSFEYDQK
                     NPVPVDSSSVLKGVKLIYVGAGSYEGYAIDENGNTYCWNENTNDLPAILNDEGVFNGN
                     KIVSMDIGGNHSCAVDVEGNVYCWGWWKGNEHGELGNGTKEASEIPVKVDDSNDLHGK
                     KIISVTVGSSHTCAIDSLGSAYCWGNNENGQIGSRKKAEPLFSPTPRDEIYTQTILKG
                     KKIILIDSSDTHTCVVDDKNKVYCWGTNDKGQLGDGTVETSYLPVAVIDDAFKDKKIK
                     FISLGMQKSCVLADNNRVYCWGNIGGHYFSGNYISSNQLVPIDITGLAYTSKATSICV
                     GDRLNAIDENGRVYAGNVIGTGDKRFVSISSYEENFYESVAIFVIDRQGTIYNWEMMY
                     DNYTSENFTKLSVDGLDEKKFV"
BASE COUNT         1119 a          504 c          859 g          978 t
ORIGIN      
        1 tatctcaaag gatggtgcgg caagaattaa aattcctccg aatgcacttg ctgaagatac
       61 ggttattact gttcagaaaa tggatgtttt aaaatatcct gatggtgaca tgattgcaag
      121 cagtgtttat aactttggtc ctgatggttt aaagtttgac aagccatcaa cactgacttt
      181 aacagccaca aaagataaac cggttggtat tggtgctgct gttgctatat tcagagatgg
      241 caagtgggtc catgagggaa gcggcgaccc gattatgtca ggcgacccga tcatgagtgg
      301 tgatcctatt atgtcaggcg atccgataat gagtggagac cccatcatgg ctgaagtcgg
      361 tcatttcacg gagttcactt tgatatattt tgatattgaa gatgaaacac cggatgaagt
      421 tgtggttgta atagacaatg agcatattga tgaagagata gttaatgagg aaccggacga
      481 ggaacatata gaaattgatg atgactatga agaaattgtt gatgaagagc cggatgatat
      541 agaagaagaa ataataatag gcgaactggc tttgattcct gccggaagtt tctggatggg
      601 ttctcctgtt gaagaaccgg ggagagaaac taatgaagtg ctccactatg ttcagatttc
      661 aaaaggtttt tatgtcggca aatttgaggt tacacaagga gagtttcaaa gtttaatggg
      721 ttataatcca agcagtttcc taacttgcgg ttctgattgt cctgttgaaa cagtgacttg
      781 gcatgaggca ttgaaatatg caaatgaact atcaaaaaaa gaaacacttg aagaatgtta
      841 tgactgttcc ggttcggcac cggatttcaa ctgttcttta aaaactgcat tttcaaaacc
      901 tcaggactgc cttggatacc ggctcccgac agaaagtgag tgggaatatg ctgccagagc
      961 aggaactgac acagcaattt acaatggagt acttacccat atcggcagaa cacctcttga
     1021 tgagaatctt aatgccatcg gatggtacgg aggcaattca aaagcaactt attcagatgc
     1081 ctataattgc agcggctggt ttaccggctc tgacaaatgc ggaactcaat ccgttggcag
     1141 taaattgaag aacaactttg atctttatga catgagtgga aatgtttggg aatggacagc
     1201 cgattttgat ggaacatatc cttctggaac tgaacttgaa ccttctgttg atccctggca
     1261 atctgtgtca agccccaaaa gagttgtaag aggcggaagc tggaacaacc ttgcttcact
     1321 ttgcagaagt gcatacagat actatcttgt aaatactgac agaacaaact atctcggttt
     1381 cagagttgca aggagtgtgg aagaataaat tgttttaaaa aaggagaaac ccgatgagaa
     1441 acacaacaac aatgatcgca attatgctta tgacggtcgt gttgaccgta tcatgcggag
     1501 aaagcaaaac aaaaaatgac aaagaatctg atattaatga cgatattagc aattccgata
     1561 atgatgaaca gacggatgtt gatagtggca atactggaaa taccggaaac accggaaatt
     1621 caggagatac gggggatacg ggaaattcag gcaatagtgg gaacacagga gatactggag
     1681 atactggaga tactggaaat acaggagaca gtgggaagtt atggattaaa atttctgccg
     1741 gaagttctca tacatgtgca attgacactg acagtaaagc ttattgttgg ggaagtaata
     1801 gttttggtca gctcggagat ggaacaaata gcgatagaat ttttccggtt gaagttgata
     1861 cagcgggtgt gttgagtaat aaaaaactaa ttttaataag ttcaggcggg aatcatacct
     1921 gtgttgttga taatgaaaac aaggtgtatt gttggggatc taacgattct ggtcaattgg
     1981 gaactggggc tgattttgga tggaatgatt ttagcaatgt tcctattgag attgataatt
     2041 cgggggtttt aaaaggagtt gcgattgttt caattagttc aggatatgac cacacatgtg
     2101 ctgtgagtaa agtgggtaaa gcatattgct ggggcgacaa tgaatacggt caattgggtg
     2161 atggaacaga gacagacaag cccactcctg ttcttgttaa tgacaaaggt gttttaaacg
     2221 ggaagttttt acttcagata agtgccggcg gaattcttac atgtactctt gatgatgaag
     2281 gaaaagcata ttgctgggga ggcggacagt acggtggttt gggagatggg tcattcgaat
     2341 atgatcaaaa gaatcctgtt cctgtagata gttcgagtgt cttaaaaggt gtgaagttga
     2401 tttatgtagg tgcaggaagt tatgaaggtt atgcaattga tgaaaacgga aacacatatt
     2461 gttggaatga aaacaccaac gatcttccag caattcttaa tgacgagggc gtttttaacg
     2521 ggaataaaat agtttcaatg gatattggag gcaatcatag ctgtgctgtc gatgttgaag
     2581 gaaatgttta ttgttgggga tggtggaaag gcaatgaaca tggtgagtta ggtaacggca
     2641 ctaaagaagc ttctgaaatt cctgttaaag ttgatgattc gaatgattta cacgggaaga
     2701 aaattatttc tgttacagtt ggctcaagtc atacttgtgc aattgatagt ttaggcagtg
     2761 cttattgttg gggtaacaac gaaaatggac agatagggag tagaaaaaaa gcagaacccc
     2821 ttttttcacc aactcccaga gatgaaatat atactcaaac aatacttaag gggaaaaaaa
     2881 ttattctcat tgattcttca gatacacata cttgtgtggt cgatgataaa aacaaagtct
     2941 attgctgggg aacaaatgac aagggacagt taggtgatgg aacagtagaa acaagttatt
     3001 tgcctgttgc agtaattgat gatgctttca aggataaaaa aattaaattt attagtttgg
     3061 gaatgcaaaa aagctgtgta cttgctgata acaatagagt gtattgctgg gggaatattg
     3121 gcgggcatta tttttcaggt aattatatct catcaaatca acttgtccct attgatataa
     3181 caggtttagc atatacttca aaggcaactt ctatttgtgt gggagataga cttaatgcta
     3241 tagatgagaa tggaagagtt tatgctggaa atgtaattgg gactggtgac aaaagatttg
     3301 tatcaatttc ttcatatgaa gagaattttt atgaatctgt ggcaatattt gtgatagaca
     3361 ggcaaggcac tatttataat tgggaaatga tgtatgacaa ttatacgagc gagaatttca
     3421 ctaaactgag tgtagatggt ttggatgaga agaaatttgt
//
LOCUS       DAOOYB010000299         3459 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_32634_length_3459_cov_6.861046, whole genome shotgun sequence.
ACCESSION   DAOOYB010000299 DAOOYB010000000
VERSION     DAOOYB010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3459
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32634_length_3459_cov_6.861046"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="PL056_12455"
     CDS             complement(<1..659)
                     /locus_tag="PL056_12455"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HPM47874.1"
                     /translation="MKKIFIISVVFAVFFIVSCGDDKKVENDDENVNDADELTDDEGI
                     TPICKTPGDGPYELKFTDITEELGLKGMDVLGSQITIADIDGDRWSDIYVTLSSKLRE
                     DPSAPKGLYRLLKNKKGNAFEDVTFTSGLFKDKEGTNGLASSYVVFADMDNDGDLDAM
                     NVVYVDKSAYTTDGYMDDRTKVYLNNGDGTFKPGPSYDFGPSMYEAFAGISFLDYDLD
                     G"
     gene            complement(755..1996)
                     /gene="fabF"
                     /locus_tag="PL056_12460"
     CDS             complement(755..1996)
                     /gene="fabF"
                     /locus_tag="PL056_12460"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HPM47875.1"
                     /translation="MNRRVVVTGMGCVTPVGSGWKNYWGNLLAGKNGISLITGFDTTD
                     FNSKIAGEIKDFDMGLYIDKTEYRRMDRYTQFAVAAAKQAIDDAGIDIETVDKDSFGV
                     YLGSGIGGMATLEEQHRILMERGPKRISPNFIPMMISNIATGHLSILFNAMGPSMTTV
                     TACASSTNAIGEALKCIQRGDADMMIAGGSEASITPLAIAGFTSMKALSTRNDAPEKS
                     SRPFDKERDGFVMAEGGGMLILEELGHALKRGAKIYAEMVGYGMAADSYHVTAPHPEG
                     KGAQRSMERAIADSGMKPEDVDYINAHGTSTYFNDMLETKAIKSVFGEHAYKLAVSST
                     KSMTGHLLGGAGAVEAIATICAIVHDEMPPTMNYEVPDPECDLDYVPNAPRKKVVNFA
                     LSNSLGFGGHNSTIAFKKYKE"
     gene            complement(2034..2270)
                     /gene="acpP"
                     /locus_tag="PL056_12465"
     CDS             complement(2034..2270)
                     /gene="acpP"
                     /locus_tag="PL056_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HPM47876.1"
                     /translation="MYFEKIVELVKEHLALDDVSNITPQTSLMGDLEVDSLDAVEIVM
                     KIEDTFGIEIPDEDVNQFKNLGDIANYIKAKKEA"
     gene            complement(2308..3051)
                     /gene="fabG"
                     /locus_tag="PL056_12470"
     CDS             complement(2308..3051)
                     /gene="fabG"
                     /locus_tag="PL056_12470"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003739222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HPM47877.1"
                     /translation="MTLKGKTAVVTGGYRGIGKAIAIKLASMGANVVINGIGAPETAD
                     DTVNELKSFGVEAQAVSANVTVEEEVNGMIAKVIEKFGHIDILVNNAGITRDGLLMKM
                     KESDWQSVLDVNLKGVFLCTKAVVRPMMQQKGGSIVNITSVVGITGNAGQANYSASKA
                     GVIGFTKSIAKEIGSKGVRVNAVAPGFINTKMTEALPEEVKKAYMANIPLKRYGEPEE
                     IAEVVAFLCEDRSRYMTGQVLKVDGGMAL"
     gene            complement(3051..>3459)
                     /locus_tag="PL056_12475"
     CDS             complement(3051..>3459)
                     /locus_tag="PL056_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="[acyl-carrier-protein] S-malonyltransferase"
                     /protein_id="HPM47878.1"
                     /translation="ENGAMKAVMLPVSAPFHSSLLKPAAEKLHEVLKNTEVNPLNKHV
                     VSNAEAKVLKSHEEVVPALVRQVNNSVLWVPSIELMIDEGVDSFVEIGPGKALSGFVK
                     RIAKSKGAKVDIYNVEDMVSFKNFVDAKNKGGN"
BASE COUNT          924 a          895 c          595 g         1045 t
ORIGIN      
        1 atcccgtcaa gatcataatc aagaaatgat atgccggcaa aagcttcata catcgaagga
       61 ccgaaatcat atgaaggtcc gggcttgaat gtgccgtctc cgttgttgag atatactttc
      121 gtcctgtcat ccatgtaacc gtcagtcgtg taggcagatt tatcaacata tactacattc
      181 atggcatcca gatcaccgtc attatccatg tcggcaaaaa caacataact tgaagcaagc
      241 ccgtttgtcc cttccttatc tttgaacaac ccgcttgtga atgtaacatc ttcaaaagca
      301 ttgccttttt tgttcttcaa caatctgtaa agacctttcg gagcggatgg atcttctcta
      361 agttttgaag aaagagtgac ataaatatca gaccatctgt cgccatcaat atcggcaatt
      421 gttatctgac ttccaagaac atccatcccc ttcagaccga gctcttcagt aatatccgtg
      481 aatttcagct cataaggccc atcccccggg gtcttgcaga tcggagttat cccttcatca
      541 tcagtcaatt catcagcatc attcacattt tcatcatcgt tttccacttt cttgtcatct
      601 ccgcatgaca caataaaaaa aaccgcaaaa acaactgaaa taataaaaat ctttttcaat
      661 ttatcctcca agaacttaat tttgacacac gaattggttt atatcacaag agcgtaaaag
      721 attcaagaaa gggaatagat taaaggatta aatgttattc cttatacttt ttgaaagcga
      781 tcgtggaatt gtgtccgccg aatccgagtg aatttgaaag tgcgaaattc acaactttct
      841 ttctgggtgc gttcggaaca taatcaagat cacattctgg atcgggaact tcataattca
      901 tcgtgggcgg catttcatca tgaacaattg cacagatcgt agcgatcgct tcaactgctc
      961 ccgctccgcc gagaagatgt cccgtcattg attttgtcga gcttaccgct aatttatatg
     1021 catgttcgcc gaaaacactc tttatcgcct tggtttcaag catatcgttg aaatatgtgc
     1081 ttgttccgtg agcattgata taatcaacat cttcaggttt catccctgaa tcggcaatgg
     1141 ccctttccat tgaccgctgt gctcccttcc cctcaggatg aggcgctgta acatgatatg
     1201 aatctgccgc catgccgtat ccaaccattt cagcatatat tttcgcccct cttttcaaag
     1261 catgaccgag ttcttcaaga atgagcattc cgccaccttc cgccattaca aaaccatccc
     1321 gttccttgtc aaacggacgg cttgactttt caggtgcatc gtttcttgtc gaaagagctt
     1381 tcatcgatgt gaaaccagct atcgcaagcg gagtaattga cgcctcgctt cctccggcaa
     1441 tcatcatgtc agcatcaccg cgctgaatgc attttagcgc ttcgcctatt gcgtttgttg
     1501 aagaagcaca tgcagttacc gttgtcatgc tcggccccat cgcattgaaa aggatcgaaa
     1561 gatgacccgt tgcaatgttt gagatcatca tcggaatgaa gttagggctt atccttttag
     1621 ggcctctttc catgagtatc cggtgctgtt cctcaagggt cgccataccg cctatgcccg
     1681 atccgagata gacaccgaaa ctgtctttgt caactgtttc aatatcaatg ccggcatcgt
     1741 cgatcgcctg tttagccgcc gcaactgcga actgagtata tctgtccatc cttctgtact
     1801 cggtcttatc aatataaaga cccatgtcaa aatccttgat ctctccggct atctttgaat
     1861 tgaaatcagt cgtgtcaaaa cccgtgataa gagatattcc gttctttcct gcaagcagat
     1921 ttccccagta atttttccat ccgctgccga ccggagtaac acaacccata ccggtaacta
     1981 caactctcct gttcattccc ctacctcact ttaatttttg aggaaaattt ttcttaagct
     2041 tccttcttcg cctttatgta atttgcgata tcgcccagat ttttgaactg gttgacatct
     2101 tcatcaggaa tctcgatccc gaaagtatcc tcgatcttca taacgatctc aaccgcatca
     2161 agagaatcga cttcaaggtc acccataagt gatgtctgcg gcgtaatgtt ggaaacgtca
     2221 tcaagtgcaa gatgttcttt aacgagttca acgatctttt caaaatacat aacaacctcc
     2281 ataaattatt tactttttaa cagctcatca aagagccatt ccaccatcaa cttttaaaac
     2341 ctgacccgtc atatatctgg atctgtcttc acacagaaac gcaacaactt ctgcaatttc
     2401 ctccggctca ccgtatcttt taagaggaat atttgccata tatgcttttt taacctcttc
     2461 cggaagtgct tctgtcattt ttgtatttat gaaaccggga gcaactgcat tcactctcac
     2521 acctttgctt ccgatctctt ttgcaataga tttggtaaaa ccgatcacac cggcttttga
     2581 tgcactgtaa tttgcctgac ccgcattacc cgttatcccg acaacagaag taatgttcac
     2641 gatacttccg cctttctgct gcatcatcgg tctgacaaca gcttttgtgc atagaaatac
     2701 ccctttcaga ttgacatcaa gaacgctctg ccagtccgat tccttcattt tcatcaaaag
     2761 tccgtctctt gtaattccag cattgttgac aagaatatca atatgtccga acttctcgat
     2821 cacttttgcg atcatgccgt tcacttcctc ttcaacagtt acatttgcag aaactgcctg
     2881 agcttcgaca ccgaacgact tgagttcatt gactgtatca tctgcagttt ccggagctcc
     2941 tataccattg atcacaacat ttgcacccat tgacgcaagc tttatagcta ttgcttttcc
     3001 tatacctctg taaccgcctg tcacaacagc ggtcttccct tttaatgtca tcagttgccc
     3061 cccttatttt ttgcatcaac aaaattttta aatgaaacca tatcttcaac attataaata
     3121 tcgacttttg ccccttttga ttttgctatt ctcttcacaa atcctgataa tgccttgccc
     3181 ggaccgatct caacgaacga atcgacccct tcatctatca tcaactcgat cgaaggaacc
     3241 caaagaaccg aattgttcac ctgcctgaca agagccggaa caacctcttc atggcttttg
     3301 agcactttcg cctcagcatt cgaaacaaca tgcttattca gcggattaac ttcagtattt
     3361 ttaagcactt catgaagttt ttcagccgcg ggcttaagaa gagaagaatg aaacggggca
     3421 cttacaggaa gcatcaccgc cttcatcgca ccgttttcg
//
LOCUS       DAOOYB010000300         3458 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_32660_length_3458_cov_4.136056, whole genome shotgun sequence.
ACCESSION   DAOOYB010000300 DAOOYB010000000
VERSION     DAOOYB010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32660_length_3458_cov_4.136056"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3458)
                     /locus_tag="PL056_12480"
     CDS             complement(<1..>3458)
                     /locus_tag="PL056_12480"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HPM47879.1"
                     /translation="CTMGKCIIEQDSKMYCGCNVDDDCVAGYYCAPDGFCSNQRCSLF
                     NTEITLFEGGCDTEGKCGGIGSLVCVSDKDCDAHKSKITCESGKCKYTSGATSTQGGN
                     CTIKADCLVCSGNNFLNYNDNAVNINQSEIEYKSTANGKVGVLVTESKCRTCETDCPT
                     TTLKWQRIISESCSGDQCSNEDYLLNEVFADPVAASSWTDVSASDPDFVQKTGDYQVF
                     SVQKDKIYRWVICSDDFDTQLTLFRGEASSDSCGTFLEYSDDAAVEMCSTGIGSIIEW
                     KSDFNGKVTLLINEYQCRQCAKSSSTMDPYGHCSDKEFDLKWQKYDCNVCSYNKEINL
                     NSGSVSNFAPSASQQFILTNVKKGNAYKFALNKSDAILTLRKGNTQNDCTGELIEQGS
                     GSLSFVSTFNGSATLVVSGKNCTALSGSTKLTVKVEYDASARFIKPYLDNSWEAIGTS
                     ADHDWECKDKNGDKTFTCCEWTGVSKDNNTGLLIYDPKKDFADWESARDYCNGLNYPV
                     IPSQTYKYCEEKLGEFDYVVCPGKDTFVCRDCADKCLSDCSSGCDQTGCSEACKNCTI
                     EQKAGHQKCVANECYERIGVGLGTEGCHTDPDRVIYPESCMKKECDEIEKCGTGTGYC
                     EVKTGAHIYDLKTGNYGGNAGKSISGWTLPTINQLYSIVDFGLFDPATSYDITGSYNM
                     SMPWYWSSTSVADSAQYAWTVDMKEGLSYRAPKTGGSSLKVICVMGASVVGEFYHFFP
                     AKHRLFEGWACDTRHPAEQIEIYFAIYDKNLANISTADTVDLPGNESIKVFSYGLTDI
                     IPDPSDTSYGDINHNCANTAGTKPHVFSLDLNSATDALAVKIKSAISSTEVNYVTVYA
                     KNHVTGAVPVTTTVIPPAQEYFVLNDSCGDSFKTGTEECDDGNTVIENCDYNNDCQTC
                     GKLCTFVQGNIRKCGDSKIQKSNCGSLTEPDCYVVEGANEACDCGQNIFTISGGICDK
                     DRGDSLTCPGYSYAGPGYTCELCNGCQITTKGLSYCGDNITNSDWGEECDNGSAGNND
                     SSQCLEDCTLATCGDGLTCSSCTPQEVCDAGGNNGEQYNNCNSGCSGYLTCGDGVIQR
                     DDCPGAVNYGDSGTYNGKVCVKVQGANEKCDEGSVNNGDYRLDYDPADPGCNATCSGV
                     APYCGD"
BASE COUNT          971 a          839 c          578 g         1070 t
ORIGIN      
        1 catctccaca atacggagca actccggaac atgttgcatt gcatcccgga tcagccggat
       61 catagtccag tctgtagtca ccattgttaa ccgatccctc atcacatttt tcattggctc
      121 cctgaacttt tacgcacact ttcccgttat atgttccgga atcaccgtaa tttactgctc
      181 cgggacaatc atctctctga atcactccat ctccgcaagt cagataacct gaacagccgg
      241 agttacaatt gttgtattgt tcaccattgt ttccacctgc gtcgcaaact tcctgcggag
      301 tacaggaaga acatgtcaat ccgtctccac atgttgcgag agtgcagtct tcaaggcact
      361 gtgacgaatc attgttcccg gcagatccat tatcacactc ttctccccaa tctgaattag
      421 tgatattgtc accgcagtaa gacaatcctt ttgttgtgat ctgacaacca ttgcaaagtt
      481 cacatgtata tccaggcccg gcatatgaat aacccgggca tgtcagagaa tcacctctgt
      541 ctttatcaca gatgccaccg cttatagtaa atatattttg accgcagtca caagcttcat
      601 ttgcaccttc aacaacataa caatccggtt cggttaaaga tccgcaattg gatttttgaa
      661 tcttcgagtc tccgcatttt ctaatatttc cctgaacaaa ggtgcataac tttccgcatg
      721 tttgacagtc attgttataa tcacaatttt caattacagt attaccgtca tcacattctt
      781 ctgtacctgt tttaaaagag tctccacatg aatcattcaa cacaaaatat tcctgtgcag
      841 gcggaataac tgttgtagtc acaggaacag cacctgttac atgatttttt gcataaacag
      901 ttacatagtt cacttcagtt gagcttatag cactctttat tttcactgcg agagcatctg
      961 ttgcagaatt caaatcaaga gaaaaaacat gcggtttggt tcccgctgta ttggcacaat
     1021 tgtggttaat atctccgtat gatgtgtccg acggatcggg aattatatct gtcaaaccgt
     1081 aactgaacac ttttatagat tcattgccgg gcagatcaac tgtgtccgca gtcgaaatat
     1141 tcgcaagatt tttatcgtat atcgcaaaat atatttcaat ctgttcagca ggatgtctgg
     1201 tgtcacatgc ccaaccctca aacagacggt gttttgccgg gaagaaatga taaaattctc
     1261 caactacaga tgcacccatt acacagatca ccttaagaga acttcctccg gttttaggtg
     1321 cacggtaaga caatccctct ttcatgtcaa ctgtccatgc atactgggcg gaatcagcta
     1381 cagatgttga actccagtac cacggcatag acatattata agatcctgtg atatcataag
     1441 atgttgcggg atcgaacaaa ccgaagtcaa ctattgaata aagctggttg atcgtcggaa
     1501 gcgtccaccc tgaaattgat tttcccgcat taccgccata atttccagtt ttaagatcgt
     1561 aaatatgtgc gcccgttttc acctcacagt atcctgttcc agttccacat ttttcaattt
     1621 catcacattc ttttttcata cagctttctg gatagattac tctatcagga tcggtgtggc
     1681 atccttccgt acccaaaccg acaccaattc tttcataaca ttcgttggca acacatttct
     1741 gatgacccgc tttctgctca atagtgcagt ttttacatgc ttcactgcac cctgtctgat
     1801 cacaacctga actacagtcg cttagacact tgtcagcaca atctctgcag acaaaagtgt
     1861 ctttaccggg acatacaaca taatcaaatt cacccaattt ctcttcacaa tatttatatg
     1921 tctggctggg aataacagga taattcaatc cgttacaata atctctggca ctttcccagt
     1981 ctgcaaaatc ttttttcgga tcataaatga gcagacccgt gttgttatct tttgagacac
     2041 ctgtccactc acagcatgtg aaagttttgt caccgttttt atctttacat tcccaatcat
     2101 ggtcagctga agttcctata gcttcccacg aattatcaag atatggcttt ataaaacggg
     2161 ctgatgcatc atactcaact tttaccgtca attttgttga tcctgacaat gcagtacagt
     2221 ttttacctga aacaacgagt gttgcacttc cattaaatgt ggaaacaaaa cttaatgaac
     2281 cgctcccctg ttctatcagc tctccggtgc aatcattctg tgtgttcccc tttctaagcg
     2341 ttaagatcgc atcagattta tttaaagcaa acttatacgc attacctttc tttacatttg
     2401 taagaataaa ctgctgagat gctgatggtg cgaaattact aactgatcct gaattcaggt
     2461 tgatctcttt attataactg caaacattac aatcatattt ctgccactta agatcaaatt
     2521 ctttgtctga gcagtgacca taaggatcca tagtactcga cgatttggca cactgtcggc
     2581 actgatattc atttataaga agcgttacct tgccgttaaa atcggatttc cattctatga
     2641 tactcccgat acctgtgctg cacatctcta ctgcggcatc atctgaatat tctaggaaag
     2701 ttccgcaact atcagatgaa gcttctcccc tgaaaagagt cagctgagta tcaaagtcgt
     2761 ctgaacatat tacccatcta tatattttat ctttttgaac actgaaaact tgataatcac
     2821 ctgttttctg aacaaaatca ggatctgatg ccgacacatc agtccaactc gacgctgcaa
     2881 caggatccgc aaaaacttca ttcaataaat aatcttcatt gctgcactga tctcctgaac
     2941 agctttcaga tattattctc tgccatttta aagttgtagt cggacaatcc gtttcgcagg
     3001 ttctgcattt agactcagtt acgagtacac cgaccttccc gttagctgta gacttatatt
     3061 caatctctga ctgattaatg tttaccgcat tatcgttgta atttaaaaaa ttgttaccgg
     3121 aacaaaccag acaatcggct ttaattgtgc aatttccacc ttgagttgaa gttgctccgc
     3181 ttgtatattt acattttcca ctctcacagg ttatcttgct tttatgtgca tcacaatcct
     3241 tatcagaaac acagaccagc gatccgatgc ctccacattt accttccgta tcgcatcctc
     3301 cctcgaagag agttatttct gtattaaaaa gagaacacct ctgattggag cagaaaccat
     3361 cgggagcaca gtaataacca gccacacagt catcatctac attgcaaccg caatacattt
     3421 tagaatcctg ttctattata cacttaccca tggtgcag
//
LOCUS       DAOOYB010000301         3419 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_33150_length_3419_cov_10.776457, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000301 DAOOYB010000000
VERSION     DAOOYB010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3419
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33150_length_3419_cov_10.776457"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="PL056_12485"
     CDS             complement(<1..238)
                     /locus_tag="PL056_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018403512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein Fic"
                     /protein_id="HPM47880.1"
                     /translation="MYKEPGSSEIILYKTEDGSVKIDTIFQNETIWLTQKKMAELFDV
                     QRPAITRHLKNIFESGELDEKVVSSILEHTTQHGA"
     gene            417..1190
                     /locus_tag="PL056_12490"
     CDS             417..1190
                     /locus_tag="PL056_12490"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPM47881.1"
                     /translation="MRYFLFLLVAMVFISCGTETKKTSDSDFLSDDVDVADELSDDQS
                     DDLSDEVLTESEVLTESEVLTESEVSDTESDETVDEDVYVPVATDFVLLKTNKGDITI
                     GLYESEVPDTVANFKQYATDKFYDGLIFHRIVPGFVIQGGGYDKDLVARETRDKIDFE
                     GSPLIKHIKYVVSMARSTSIHSATSQFFIMLDTFPHLDYTSDEDFMDKEKFPCTAFGI
                     VTDGFEIVDEIAEVETETQGMFEGVPVEPVIIESAELIN"
     gene            complement(1196..1729)
                     /locus_tag="PL056_12495"
     CDS             complement(1196..1729)
                     /locus_tag="PL056_12495"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPM47882.1"
                     /translation="MDEKFHLSSELEKKRYELHNYDIHDAGYRNFTAPLINAVLKNFN
                     NQHSGLDYGCGRVPVISEILKENGYLIKIYDPFFHPDTKPLEDKYDYIICCEVFEHFR
                     HPDKEMIKLKNLLKPNGKLYIMTHIYAEPTNFSKWYYRNDPTHIFIAQPETFKYIEKE
                     FCMKIVSMNDRLVIFEI"
     gene            2007..2672
                     /locus_tag="PL056_12500"
     CDS             2007..2672
                     /locus_tag="PL056_12500"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HPM47883.1"
                     /translation="MDYRMPLLGDKFPEMKVQTTHGMKMLPNDYEGKWFVLFSHPADF
                     TPVCTTEFVAFQKRYDKFRELNCELIGMSVDQVFSHIKWEEWIKEKIGVEIQFPIIAD
                     TGAVAEKLGLIHPGKGTNTVRAVFIVDAKGNIRIIFYYPQELGRNMDEILRAVKAMQV
                     SDTNKVAMPAQWPDSEVVGKDHVIIPPATDVKTAKERLEKAKAGEFECLDWWLCHKKL
                     EVK"
     gene            complement(2680..>3419)
                     /locus_tag="PL056_12505"
     CDS             complement(2680..>3419)
                     /locus_tag="PL056_12505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47884.1"
                     /translation="SEMQSYVDLSNPEDERYFRHDDCSYSDKGIDDPNIQETIAWHAS
                     LDIDNTWGGNNFETPETINLGPIDNKDRDGIPDETIYDDEYLIVVGYVNCTSQYADKI
                     DRCKPDYTGVDAAGEVDARVEIMVDGVEVPRIARGLIPADNFSETTKNFKIKFQEWKV
                     IAVIKWDGTLSSPETNPKYPGSAIVSDIAMADFGIETDAKSYKTCNFQLFDTNLVPIW
                     NQQDYYDWVNAKRNPEDPSSETIGECY"
BASE COUNT         1011 a          618 c          712 g         1078 t
ORIGIN      
        1 ttgcaccgtg ttgagtggta tgttccaaaa tggaactcac cactttttca tccagttctc
       61 cgctttcaaa aatgtttttc agatgtctgg ttattgccgg tctctggaca tcaaaaagct
      121 ctgccatctt tttctgtgtc agccagatgg tttcattctg gaatattgta tctattttga
      181 cagagccatc ttctgttttg tagaggatta tttctgaact gcctggttct ttgtacatta
      241 atcttcttcc tttttcactg aactgaaaaa ctataagaaa ttaaacaact ttggtcaagg
      301 aaaaacacaa gcagtttatg attgcgtgga aaggtggaaa cttctctttg tttccggtta
      361 attggacaac ttttctaaaa taaatagaat tgtgaaagaa attttggagg tgcttaatgc
      421 gttattttct gtttttactt gttgcgatgg ttttcatttc atgcggaacg gaaactaaaa
      481 aaacaagtga cagcgatttt ttatccgatg atgttgatgt tgctgatgaa ttatccgacg
      541 accagtccga cgacttgtcc gacgaagttt taacggagtc ggaagtttta acggagtcgg
      601 aagttttaac ggagtcggaa gtgtctgata cggagtcgga tgaaactgtt gatgaggatg
      661 tgtatgttcc tgttgcgact gattttgtac tgctgaaaac caacaagggt gacatcacga
      721 ttggtcttta tgaaagtgaa gtgcctgata ctgtagcgaa ttttaagcag tatgcaactg
      781 acaaatttta tgacgggctg atatttcacc gcatagttcc gggatttgtt attcagggcg
      841 gaggatatga taaagacctt gttgcaagag agaccagaga taaaatagat tttgaaggaa
      901 gtccgctgat caagcatatc aaatatgtcg tttcaatggc aagatctaca agcattcatt
      961 ccgcaacatc acagtttttc ataatgctcg acacatttcc ccaccttgat tacacaagtg
     1021 atgaagactt catggataaa gagaaattcc cctgcacagc ttttggaatt gtgacagatg
     1081 gctttgaaat cgttgatgaa atagctgaag tcgaaactga aacgcaagga atgttcgaag
     1141 gagtccccgt tgaacccgtc atcattgaaa gcgcagagtt gattaattaa ggtttctata
     1201 tttcaaaaat cacaaggcgg tcattcattg agacgatttt catgcagaac tctttttcta
     1261 tgtatttaaa agtttcaggt tgggcaatga aaatatgggt ggggtcattt cggtaatacc
     1321 attttgaaaa gtttgtcggt tccgcataaa tatgagtcat tatataaagt ttaccatttg
     1381 gtttgagaag gtttttcagc ttgatcatct ccttgtcagg gtgacgaaaa tgttcaaaga
     1441 cttcacagca gataatataa tcatatttat cttcaagtgg ctttgtgtca ggatgaaaga
     1501 acgggtcata aatttttatc aagtatccgt tttccttgag tatttctgaa atgacaggaa
     1561 ccctgccgca accgtagtca agacctgagt gctgattatt aaaattttta agaacggcat
     1621 taattaaagg agctgtaaaa tttctgtatc ctgcatcatg gatgtcatag ttatgcagtt
     1681 cataccgctt tttttcaagc tctgaagata agtggaattt ttcatccatc acaaatgcct
     1741 tgcacttttg acagatgaaa aacctgtcat ccgccttatc agttaaatgg gttttacaaa
     1801 gtgtgcagtt catgtcgttt aaagaccgtt taatctgaaa aaaacattga tactatattg
     1861 ccgattatct tattatcaag aagaatcata gttgttttta tcgaagtaat tcagaaaatc
     1921 agggtaaaag gggttgaagt tttaaaatga tgtgtatata attatgcgca ttaagctttt
     1981 tattaacttt tttaagaggt tacaacatgg attacagaat gccgttactt ggtgacaaat
     2041 tcccggaaat gaaggttcag acaactcacg gaatgaaaat gctccccaat gattatgaag
     2101 ggaaatggtt cgttcttttc agccatcctg cagattttac accggtctgc acgacagagt
     2161 ttgtcgcttt ccagaaaaga tatgacaaat tcagagaact taactgtgag cttatcggaa
     2221 tgagcgttga tcaggttttt tcacacatta aatgggaaga gtggatcaag gaaaagatcg
     2281 gtgttgaaat tcagttcccg atcattgctg acacaggtgc tgttgctgaa aagctcggac
     2341 tcattcatcc cggcaaaggg acaaacacag tcagagcagt attcatcgtt gatgcaaaag
     2401 ggaacatcag gataattttc tactatccgc aggaactcgg cagaaatatg gacgagattt
     2461 taagagcagt caaagcaatg caggtgtccg ataccaacaa agtcgcaatg ccggcacagt
     2521 ggcccgacag cgaagttgtc ggaaaagacc atgtcataat tcctcctgca actgatgtga
     2581 aaactgcaaa agagcggctc gaaaaggcaa aagcaggcga gtttgaatgt cttgactggt
     2641 ggctctgtca caaaaaactc gaagttaaat agaatattgt cagtaacatt caccgatcgt
     2701 ttctgaacta ggatcttcag gatttctttt tgcattaacc cagtcgtagt aatcctgctg
     2761 attccatatc ggaacaagat ttgtgtcaaa taattggaaa ttacatgttt tgtaactttt
     2821 tgcatctgtt tcaatgccga aatcagccat tgcaatatca ctcacaatgg cactgcctgg
     2881 atacttagga tttgtttcag gtgaagaaag agttccatcc cattttatta ccgcaataac
     2941 tttccattcc tggaatttta ttttaaagtt ctttgttgtt tctgaaaaat tgtcagcagg
     3001 gataagtcct cgtgcaattc ttggaacttc aacgccgtca accattattt caactcttgc
     3061 gtcgacttca ccggccgcat caacacctgt ataatcgggt ttacatctgt caattttgtc
     3121 agcatactgt gatgtacaat taacataacc gacaacaata agatactcat cgtcataaat
     3181 tgtttcgtcg ggaatgccgt cacggtcttt attgtcgatc ggtccgagat ttattgtttc
     3241 aggagtttca aaattgtttc caccccaggt gttatctata tcgagtgatg catgccatgc
     3301 aatggtttcc tgaatattag gatcgtcaat gcctttatcg gagtaggaac agtcatcatg
     3361 tctgaaatat ctttcatctt ctggatttga tagatctaca taagactgca tctctgagg
//
LOCUS       DAOOYB010000302         3412 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_33246_length_3412_cov_5.817396, whole genome shotgun sequence.
ACCESSION   DAOOYB010000302 DAOOYB010000000
VERSION     DAOOYB010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3412
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33246_length_3412_cov_5.817396"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="PL056_12510"
     CDS             <1..438
                     /locus_tag="PL056_12510"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HPM47885.1"
                     /translation="YDDGPCICSSKDRFCHEYKGLNWSELSFTQNWTEASSYCKQIKG
                     RLPTISELRMLIKNNPATETGGDCGVTDDCLSEKECLSGYDDCRGGNWDDKPHSYFGD
                     RHDMWSSSEESDDKSLVWYVNFYGATIYTSVKTNELNFRCVGE"
     gene            complement(454..915)
                     /locus_tag="PL056_12515"
     CDS             complement(454..915)
                     /locus_tag="PL056_12515"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HPM47886.1"
                     /translation="MKDFTIREIVEYAEKIEAESHKFYALAAQMIKEPGTKKLTLELA
                     EEETKHFNHLRDLLNRERLTSLELDIVVAKGINLLNRTVKTAEITEDSDPIDVLEVAL
                     EREINTEQLYTMYLTFTDIPDNMVKVFEDLKHQEIGHQNRIKTLIKKITGN"
     gene            complement(912..2702)
                     /locus_tag="PL056_12520"
     CDS             complement(912..2702)
                     /locus_tag="PL056_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011713827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPM47887.1"
                     /translation="MKIKEVLYPLSVWKNLIKQPVTILDPINREAADRYRGFHRNDMK
                     KCIGCGTCETICQNAAIDMVPVEGVETKDGDSGLRPRIDYGRCCWCALCVDICATGSL
                     CMSNKYQWVDADSDAFRFTPGADKKYWDNVEKGYKRPENYTLNPLDRVHMEELHPHER
                     NDSFIEIVKGYSMEQALKEADRCVSCGICIATCPAHMGIPRYIEAIRKNDIEEAFRVL
                     YETNPLPEVCGRVCTHKCETVCAISHRGDAVSIRWLKRYIADQVSIEKYKEILNADIL
                     ESGKKVAIIGAGPAGLSAAYYLRHLGHSVTIFEALPEAGGMMRYGIPEYRLPYDQIDK
                     DVQHILDLGVELKTNVRIGKALSFETLKKDFEAVFTSTGLHLGRSTRIDGSDHKSVYQ
                     AVDLLRDITNGKEIPHTGRIVVIGGGNVAMDIARTLARLQMKNFGKVTITLTCLETED
                     IMPADREEIVESREEGVVIMPGFGPKKIEIENGSIKGLSVVKCLSVFDENKRFNPKFD
                     ESAKEFFAADMIIESIGQGMDLSYIPENMKTEIKFDPQGRILIDEYYQSSVPWLFAGG
                     DIVEGPDVIHAIANGHIAAKSIDRYLRGEK"
     gene            complement(2706..>3412)
                     /locus_tag="PL056_12525"
     CDS             complement(2706..>3412)
                     /locus_tag="PL056_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HPM47888.1"
                     /translation="YILDRFEELTGGRIYHMYILPGGVRNDLPVGFKERLLDNLKDIE
                     QVMKDVDNALFTNAVFKKRTVGLGYIDPSWIEPFGITGPNARAAGIPLDVRKDAPYLK
                     YDELDFEPVTGKDSDAFTRADVRRRDLLMSVDLIRQILDKIPQNGEFIAKLPNVLNWE
                     IPKGETYVKSECTRGEYGYYMVSDGSMYPRRINVRGPSYTHAVALMEKLAIGVNIADT
                     AALMVSLHTYPPEIER"
BASE COUNT          868 a          788 c          684 g         1072 t
ORIGIN      
        1 tatgatgacg ggccatgtat ctgttcatcg aaagacagat tctgtcacga gtataaaggg
       61 ctgaactggt ctgaactttc attcactcaa aattggacgg aagcatcaag ttactgcaag
      121 cagatcaaag gtcgtcttcc gacaatatca gagctgagaa tgcttattaa aaacaatccg
      181 gcaactgaaa ccggagggga ttgcggagtg accgatgact gtctttcaga aaaggaatgc
      241 ttgagtggtt atgatgattg ccgtggtggt aactgggatg acaaacctca ttcttatttt
      301 ggtgacagac atgacatgtg gtcttcttcg gaagaatctg acgataaaag cctagtatgg
      361 tatgtcaatt tttacggagc tacaatatac acaagtgtca agacgaatga attgaatttc
      421 cgctgtgtag gtgaatgatc gcgattctgc aaactaattc ccggtgatct tttttataag
      481 tgtcttgatt ctgttctgat gaccgatctc ctgatgtttt aggtcttcaa aaactttgac
      541 catgttgtca gggatgtcgg taaatgtgag atacattgta taaagctgtt cggtgttgat
      601 ctcccgttca agtgcaactt caagaacatc tatggggtct gaatcttcag ttatttcagc
      661 agttttaaca gttctgttca acaggttgat cccttttgcg acaacgatgt caagttcaag
      721 tgaagtgagc cgctccctgt tgagaagatc gcgcagatgg ttgaaatgtt ttgtctcttc
      781 ttctgccagt tcaagtgtaa gcttttttgt gccaggctct ttgatcattt gtgccgccaa
      841 tgcatagaac ttgtgacttt cagcttcaat tttctcagca tattcaacta tttccctgat
      901 tgtaaagtct ttcatttttc acctcttaaa tatctgtcaa tactttttgc cgcaatgtgt
      961 ccgtttgcga tggcgtgaat tacatcaggt ccttcaacaa tgtctccgcc ggcaaaaagc
     1021 cacggaactg agctttgata gtattcatca atgagaatcc ttccttgcgg atcgaattta
     1081 atttcagttt tcatattttc aggaatatag cttaagtcca ttccctgacc gatagattca
     1141 atgatcatgt ctgcggcaaa gaattctttt gccgattcat caaatttcgg gttgaaccgc
     1201 ttgttttcat caaaaacaga aagacatttc acaacgctta gtcctttgat cgaaccattt
     1261 tcgatctcaa ttttcttcgg accgaatccc ggcatgatga caacaccttc ttcccttgat
     1321 tcgacgatct cttctctatc cgcaggcatg atatcctcag tttcaagaca ggtgagcgtt
     1381 atggtcactt tgccgaaatt cttcatctgt aaccgtgcaa gggttcttgc aatatccatc
     1441 gcgacatttc caccgccgat gacaacgata cggccagtgt ggggaatctc tttcccgttt
     1501 gtgatatctc taagcaaatc taccgcctga taaacagatt tgtgatcaga gccgtcaatc
     1561 ctcgtacttc tgccaagatg aagtccggtt gaagtgaata cggcctcaaa atcttttttg
     1621 agagtttcaa aactcaatgc ttttccaatt cttacatttg tcttaagttc aacacccaga
     1681 tcaagaatat gctgaacatc cttatctatc tggtcgtaag gaagacggta ttcaggaatt
     1741 ccgtaacgca tcattccgcc cgcttccggc agtgcttcaa aaattgttac actgtgaccc
     1801 agatgcctga ggtaatatgc cgcagaaagt cctgccggac cggctcctat gatggcgact
     1861 tttttgcccg attccaatat gtcggcattt aatatttctt tatatttttc aatacttacc
     1921 tgatcagcga tatatctttt aagccagcgg atcgaaacag catcgccacg gtgtgaaatt
     1981 gcgcatacag tctcgcattt atgggtgcag accctgccgc agacctcagg aaggggattt
     2041 gtttcgtaaa gaactctgaa agcttcttct atatcatttt ttctgatcgc ttcgatgtag
     2101 cggggtattc ccatgtgagc cggacatgtt gcaatgcata ttccgcaact gacacatctg
     2161 tcggcctctt ttaatgcctg ttccatgctg taacccttta caatctctat gaaactgtca
     2221 tttctttcat gcggatgaag ctcttccatg tgaactctgt caagcggatt gagcgtgtaa
     2281 ttttcaggtc gtttgtatcc tttttcaaca ttatcccagt attttttgtc agctcccgga
     2341 gtaaatctga acgcatcgct gtccgcatca acccactgat atttgttaga catgcaaaga
     2401 gagccggttg cacagatatc aacgcagagt gcacaccagc agcaacgacc gtaatcaatt
     2461 ctcggtcgca gtccgctgtc accatctttt gtctcaactc cttcaaccgg aaccatgtca
     2521 atggcggcat tctggcaaat tgtttcgcaa gttccgcaac cgatacactt tttcatgtcg
     2581 ttacggtgga accccctgta tctgtcggca gcttcgcggt tgattgggtc aaggatcgta
     2641 accggctgtt ttatcaggtt tttccagact gataagggat ataatacttc ttttattttc
     2701 attgatcacc tctctatttc aggtggatat gtgtgcaggg aaaccatcag tgcggcagtg
     2761 tctgcaatat ttactccaat cgcgagcttt tccatcagtg cgacagcgtg ggtgtaactc
     2821 ggacctctta catttattct ccgcggatac attgaaccgt ctgaaaccat gtaataccca
     2881 tattctcctc ttgtacactc acttttcaca taggtttcgc cttttggaat ttcccagtta
     2941 aggacatttg gaagttttgc aatgaattcg ccattttgag gaattttatc aagtatctgt
     3001 cttatcagat caacactcat cagcagatct cttcttctca catctgccct tgtgaatgca
     3061 tcactatctt tccctgtcac cggctcaaaa tcaagttcat catatttcag atagggggca
     3121 tctttcctca catccagcgg aattcccgcc gctcttgcat tcggtccggt tattccgaac
     3181 ggctctatcc atgaaggatc aatatatcca aggcccacag tccttttctt aaaaacggca
     3241 ttggtgaaaa gggcgttgtc aacatctttc atcacttgct cgatatcttt gagattatca
     3301 agaagccgct ctttgaaacc aacaggcaga tcatttctaa ctccaccggg aagaatatac
     3361 atgtgatata tcctgccacc cgtaagttct tcaaatctat caagaatata gt
//
LOCUS       DAOOYB010000303         3412 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_33248_length_3412_cov_4.504021, whole genome shotgun sequence.
ACCESSION   DAOOYB010000303 DAOOYB010000000
VERSION     DAOOYB010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3412
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33248_length_3412_cov_4.504021"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..902)
                     /locus_tag="PL056_12530"
     CDS             complement(51..902)
                     /locus_tag="PL056_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004231275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HPM47889.1"
                     /translation="MKILVLGANGMAGHTISHYFIEKGHETTTFSMAPVSFGKNVVGN
                     ALDSAFFKSLLLSDDFDAVINCIGLLVKDCEDHPDKAVYLNSYLPHFAVQILKGKKAK
                     FIHMSTDCVFAGNSGPYYEDSFSDGELFYDRTKALGEINDSKNLTFRNSIIGPDMNES
                     GIGLFNWFMKQNGEINGFTKAIWTGVTTITLAKAMERAIETDLTGLKNLVNNENISKF
                     ELLSLFNKHFKDGRVTIHRKDDLSVDKSLITKREDFDFVVPSYEQMVVEMKDWVYNHK
                     DIYPHYF"
     gene            complement(1062..2072)
                     /locus_tag="PL056_12535"
     CDS             complement(1062..2072)
                     /locus_tag="PL056_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004465101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HPM47890.1"
                     /translation="MLKDKTLMITGGTGSFGNAVLNRFLKTGVKEIRIFSRDEKKQED
                     MRIEYKNDKIKFYIGDVRDFESINSAMRGVDFVFSAAALKQVPSCEFFPVEAVKTNIL
                     GTDNTLKAAIENGVKKVVVLSTDKAVYPINAMGMSKGLMEKVAIAKSRTVPEGGTTIC
                     CTRYGNVMASRGSVIPLFVQQIHQGLPITVTDPDMTRFMMSLDDAVDLVMYAFEHGVQ
                     GDLFVQKSPASTIKMLAEVMVKIFNAKNEIKIIGTRHGEKKYETLVTREEMQKAEELD
                     NYYRIPADNRDLNYQKYFTEGEKMPELIVDYNSDNTYRLNGKELEELLMKLPFIKEEL
                     NG"
     gene            complement(2065..3297)
                     /locus_tag="PL056_12540"
     CDS             complement(2065..3297)
                     /locus_tag="PL056_12540"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPM47891.1"
                     /translation="MKVLIISETFPPLIRASARIMKDLAVTFVENGHEVSVVTFIENH
                     NEFKHITTGRFEENGYDVIRVKIPKQRGVGYFRRGITEELIPFIATRKSLNYLKNEKF
                     DLIVAHLPPLSICSMVKKLKKINNCPVYLLLRDIHPQTGVDLGIYKLGGIVFSRFRHL
                     EKKLYEISDVIAPQSPANREFILKNNPGIPPEKVSVIYNFKNPDIEPTHSINFKDKLG
                     LNGKMVCLYGGNMTSSQDLKELLMVAQKCRELKDVVFLMIGFGKEREMLNQKANEMGL
                     TNIIFEDPLPLDVFTSLVYQCDIGLMFLNKNFTTHNFPGKTLDYMNASLPIIASVNEG
                     NDLKKMIESDANCGFVHYGSDLDGVAENIKTLSLNSEKRKTLGKNGRIYLEKELNSQI
                     AYDKIMSDINKLKGDNNA"
BASE COUNT          980 a          707 c          597 g         1128 t
ORIGIN      
        1 ggggagttat tcaaagagtg cttgagggag ttattcattt atctttcctc tcaaaaataa
       61 tgaggataaa tatctttgtg gttatacacc caatccttca tttctacaac catttgttca
      121 tagctcggaa caacgaaatc aaagtcctct ctttttgtta taagtgattt gtcaactgaa
      181 agatcatctt ttctgtgaat ggttaccctg ccatctttaa aatgtttgtt aaaaagcgac
      241 aacagttcaa atttgctgat gttttcattg tttacaagat tttttaagcc agtcagatct
      301 gtctcaattg ctctttccat tgcttttgca agagttattg ttgtgactcc tgtccagatc
      361 gcttttgtga acccgttgat ttcaccattt tgcttcataa accagttgaa aagaccaatc
      421 ccggattcat tcatatcggg accgattatt gaattgcgga aagtaaggtt ttttgaatcg
      481 tttatttctc caagtgcctt tgtcctgtca taaaagagct ctccgtcaga aaaactgtct
      541 tcatagtaag gtcctgaatt tcctgcaaaa acacaatctg tactcatatg aatgaattta
      601 gcttttttac cttttaaaat ctgcactgca aaatggggaa gatagctatt taagtaaact
      661 gctttatcag gatgatcttc gcaatcttta accaaaagac ctatacagtt tatcaccgca
      721 tcaaaatcat cactgagtaa aagcgattta aaaaacgcac tatcaagagc attcccgaca
      781 acattttttc caaaactaac cggtgccatc gaaaaagtag tagtttcatg ccccttttca
      841 atgaaataat gggaaatcgt atgtcccgcc atcccatttg caccgagaac gaggattttc
      901 ataaattacc tcctttgact ccccgggcct gcgactgaag tctccgaccg cgggctaagc
      961 aagcaccgtc cttcagacgg ttaaatgaca ggtggcgggc tgaaggctcg cgatatctta
     1021 gcccacgccc aaagggctgg gaaatgattg agggagttat cttacccatt caactcttcc
     1081 ttaataaacg gtagtttcat cagcagttcc tcaagttctt ttccattgag ccgatatgtg
     1141 ttgtctgaat tgtaatcaac aataagttcc ggcatctttt ccccttccgt gaaatatttc
     1201 tgatagttaa gatctctgtt gtctgcagga attctataat aattatcaag ttcctccgct
     1261 ttctgcatct cttctcttgt cacaagtgtt tcatatttct tttcaccgtg acgggttccg
     1321 atgatcttga tctcattttt agcgttgaaa atcttaacca tcacttcagc aagcattttt
     1381 attgttgaag caggggattt ctgaacaaaa agatctccct gaactccgtg ctcaaatgca
     1441 tacatcacaa gatcaactgc atcatcaaga gacatcatga atcttgtcat gtccggatct
     1501 gtgacagtga tcggaagtcc ctgatgaatc tgctgaacaa acaaaggaat gactgaaccg
     1561 cgggatgcca tgacatttcc atatcttgtg cagcaaattg tagttccgcc ctcaggaaca
     1621 gttctgcttt ttgcgattgc aactttttcc atcagacctt tgctcattcc cattgcattt
     1681 atcggataga ctgctttgtc agttgaaaga acaaccactt ttttaacacc gttttcaatt
     1741 gccgccttaa gagtgttgtc cgtccctaaa atattggttt ttaccgcttc aacaggaaag
     1801 aattcgcaac ttggaacctg cttgagagct gctgcactga aaacaaaatc aacaccacgc
     1861 attgcagaat taatgctctc gaaatcccgc acatcaccga tatagaactt gatcttgtca
     1921 tttttatact caattctcat atcttcctgt ttcttctcat cccgtgaaaa aattctgatc
     1981 tctttgacac cagtctttaa aaaacggttg agtaccgcat ttccaaatga acccgttccc
     2041 cctgtgatca ttaaagtttt gtctttaagc attgttgtct ccttttaact tatttatatc
     2101 actcattatt ttgtcataag caatttgtga gttcaattct ttttcaagat aaattctgcc
     2161 gttttttcct agagtttttc ttttttcaga atttaaagaa agagttttta tgttttctgc
     2221 tactccgtca agatcagatc cgtagtggac aaaaccgcaa tttgcatcac tttctatcat
     2281 ctttttcagg tcgttgccct catttactga tgcaataatt ggaagtgatg cattcatgta
     2341 gtcaagtgtt tttcccggaa aattgtgtgt tgtgaaattt ttgttaagga acatcagtcc
     2401 gatgtcacac tgataaacca aagatgtgaa aacatcaagc ggaagcggat cttcaaaaat
     2461 tatatttgtg agtcccattt cattggcttt ctgattaagc atctctcttt cctttccaaa
     2521 gccgatcatg agaaaaacaa catctttcaa ttctctgcat ttttgagcaa ccataagcaa
     2581 ctctttcaaa tcctgagaag aagtcatatt tccaccgtaa aggcagacca tttttccgtt
     2641 taatccaagc ttatctttga aatttattga atgtgtcggc tcaatgtcag gatttttaaa
     2701 attatagata actgaaactt tttcaggtgg aatgccggga ttgtttttta aaatgaactc
     2761 tctgtttgcc ggagattgag gagcaataac atctgaaatt tcataaagtt ttttctcaag
     2821 atgtctaaaa cgggaaaata caattccccc taatttataa attccaagat ccactccagt
     2881 ctggggatgg atgtctctta aaagaagata aactggacag ttatttattt tttttaattt
     2941 tttaaccatt gaacagatcg ataacggagg aagatgagca actatcagat caaacttctc
     3001 atttttgaga tagttcagtg atttccgggt tgcgatgaag gggatgagtt cttcagttat
     3061 tccccttctg aaataaccga cacctcgttg tttaggaatt ttcacacgga tcacatcata
     3121 tccgttctct tcaaaccttc ctgttgtgat gtgcttgaat tcattgtgat tttcaatgaa
     3181 agtcacaact gaaacttcat gcccgttctc aacaaatgtt acggccagat ctttcattat
     3241 ccttgcagaa gcacgaatta aagggggaaa tgtttctgat attataagta ctttcataat
     3301 ctcatctcaa aaacaactca aagcattaaa atacctgatg attataaaaa aagggagaag
     3361 gaatgcaaga aatgggtgaa gcgtgatgcg tgatgcgtga tgcgtgacgg gt
//
LOCUS       DAOOYB010000304         3401 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_33402_length_3401_cov_13.401076, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000304 DAOOYB010000000
VERSION     DAOOYB010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3401
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33402_length_3401_cov_13.401076"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="PL056_12545"
     CDS             <1..597
                     /locus_tag="PL056_12545"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HPM47892.1"
                     /translation="SVPIAVALMLKGLNPGAALVFLMAGPATNAATVLVIGKTLGKKA
                     LFSYLFSIIAGALISGAIIDSFLPAEWFSVQLMNAHSHDHGALHYLYLISSIILAVLL
                     LMSFFRYLTRKKEAAHCHDKECCCENANETGELIVIHVEGMTCSHCAANVKSGLLGVS
                     GVEDAVVDHHSGKVQITGSHINMDEIKKAVESRGYKVV"
     gene            594..>3401
                     /locus_tag="PL056_12550"
     CDS             594..>3401
                     /locus_tag="PL056_12550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47893.1"
                     /translation="MIRNVAAFLIVTVFSIQVISAHEAVKFRKYETKHQVLYFSSNLD
                     DSVIPRLIKQAVKSEEFIKDLYGWVPQKKIITVYDRETDTANGWSRSYLKNVIYLYTY
                     PPERYSTLSSYTSWEQGLHVHEYTHSVQIGNTKGFPKFINTVFGNLYFPGGMIPRWAL
                     EGAAIYSESLIEGKGRLNSPLYKAHFDSFFLKGKELSLGELSGIPDHWMGGGLPYLYG
                     TYFYAYLVEKTGKKQMAAFFDELSDDAFPFLANRAARTTLGSSLTDLYKKFIREKKDE
                     LSSKVEDSSGEHGSKERFHSVFVDLNSDGYVFSGEKISKRGIYRFNEGELKKLSSFPS
                     HESFSKKDEKYVFPLNIRHKERYYRNEIFYADLKKRSIKKITENETAGEVVFGSDNDI
                     YYTSFRDGINRITRMKLDGTVISEWDFRELDSIFSISLSSDGKKMVFTGNLYNFEKNI
                     FIFDTENSELTEVRIDGDQYSVYFRNDDEIVFSAEKNGRIVPMSLNFKEKRLKQLHRP
                     SSVAIFPKVINDEIFFIAFDNDGYYPAWQKTENIDLGKVPDGVFVTVERKVKEFEKVT
                     LKDAKFYEGMFPALIIPDYQGSVNSHTIGFTVWGESNDQERFYDISYYKTFGQTGRHF
                     AILNYFDEAVWPGFRWYFSYSREDFKTGGFYRTDAAYNRFNSGVSLSSSTSSPVFILP
                     SKIVNMNHSIRSGLGLSGTDMNVKQLKDPLDITPSEKDRLDITAYFSYGFSFSFNPGS
                     YYLFSNMERTSFSFPVTIYRSLMGDDRSLVFSPSMQLSFLILPNGKLGFITRNVLYMR
                     FLTDNYFLLGGDELDIDILNLNTFIYGGSSSVTVRGYNYGAAGGKHVYHSNNELRFHL
                     FSISQGFNTFPLMFRNVQGALFFDIGSGSPDINMFDDKFIAGIGAELKLYSVWWYRVP
                     IIFTFGTAYGLTKSGQLNFYFS"
BASE COUNT         1012 a          645 c          730 g         1014 t
ORIGIN      
        1 tctgttccga tagcggttgc tcttatgctg aaagggctta atccgggtgc ggcactggtt
       61 tttctgatgg caggacctgc aactaatgcc gcaacggttc ttgtgattgg caaaacgctt
      121 gggaaaaaag ctcttttcag ctatcttttt tcaattattg ccggggctct gatatcagga
      181 gcaattattg acagtttcct gccggcagag tggttttctg ttcagttgat gaatgctcat
      241 tctcatgatc atggagcact gcattatctt tatcttatct cctcgatcat tcttgccgta
      301 ttactgctga tgagtttttt cagatatctt acaagaaaaa aagaagccgc acattgccat
      361 gacaaagaat gctgctgtga aaatgcaaat gaaaccggag agttaattgt aattcatgta
      421 gaggggatga cttgcagtca ctgtgcggca aatgtaaaat cggggctgct cggtgtttca
      481 ggagttgaag atgcggttgt agatcaccac agcggtaaag ttcagataac tggatctcat
      541 atcaacatgg atgaaataaa aaaagcggtt gaatcaaggg gttataaagt cgtatgatta
      601 gaaatgtcgc tgcattttta attgttacgg ttttttcgat acaggtaatt tcagctcatg
      661 aagcagtcaa gttcagaaaa tatgaaacaa agcatcaggt gctctatttt tcatccaatc
      721 ttgacgattc ggtcattcca cggctcatta aacaggcggt aaagtcagaa gagtttatca
      781 aagatcttta cggctgggtt cctcagaaaa aaataatcac agtttatgac agagagaccg
      841 atacagcaaa cggatggtca aggagttatc ttaaaaatgt aatatatctc tatacttatc
      901 ctcctgaaag atattcgaca ctttcaagtt atacaagctg ggaacaggga ctccatgttc
      961 acgaatacac ccattccgtt cagataggga acacgaaagg attcccgaaa tttatcaata
     1021 ctgttttcgg caatctctat tttccgggcg gaatgattcc aagatgggct ctcgaaggtg
     1081 cggcaatata cagcgaatct ctcattgaag ggaaggggcg gctcaacagt cctctgtaca
     1141 aagctcattt tgattctttt tttctgaaag ggaaagagtt gtcgctcgga gagctttcgg
     1201 ggatacctga tcactggatg gggggaggac ttccttatct ttacgggact tatttctatg
     1261 cgtatcttgt tgaaaagact ggaaaaaaac agatggcagc tttttttgac gaattgtcgg
     1321 acgatgcttt tccttttctt gccaacagag ccgcaagaac cactctcggt tcatctttaa
     1381 ccgatctcta taagaaattc atcagggaaa aaaaagatga attaagttca aaagtggagg
     1441 actcttcggg agaacacggt tcaaaagagc ggtttcattc ggtatttgtc gatctcaatt
     1501 cagatggtta tgtgttttcg ggtgaaaaaa tatcaaaaag agggatatat cgcttcaacg
     1561 aaggtgaact taaaaaactt tcatcttttc cgtcccacga atcattttcc aaaaaagatg
     1621 aaaaatatgt ttttccctta aatatcaggc ataaagaaag gtactacagg aatgagatat
     1681 tttatgccga tttaaaaaag cggtctataa aaaagataac agaaaatgaa actgcaggcg
     1741 aagtcgtttt cggatctgac aacgatattt attacacttc gttcagagac ggtataaacc
     1801 gcatcacccg catgaaactt gacggaactg tgatcagtga atgggatttc agagagctcg
     1861 attcgatttt cagcatctct ttgtcaagtg acggaaagaa aatggttttt accggcaatc
     1921 tttataattt tgaaaagaac atattcattt ttgatactga aaacagcgag ttgactgaag
     1981 tgaggattga cggtgaccag tattcggtat atttcagaaa tgatgacgag atcgtttttt
     2041 cagccgaaaa gaacggaaga attgttccga tgtcacttaa ttttaaggaa aaacggttaa
     2101 aacagcttca caggccctct tctgttgcaa tttttcctaa agttataaat gacgagatat
     2161 tttttattgc ttttgacaat gacggctatt atccggcatg gcagaagaca gagaacatcg
     2221 accttggaaa agtgccggac ggtgtttttg taacagttga aagaaaagtt aaggaatttg
     2281 agaaagttac acttaaggat gcaaaatttt atgaagggat gttccccgct ctcataattc
     2341 ctgactatca gggttctgtt aattcccaca caatagggtt tacagtatgg ggagaatcca
     2401 atgatcagga aaggttttat gatatttcct attacaaaac tttcggtcaa acgggaagac
     2461 attttgcgat actgaactat tttgatgaag ctgtctggcc cggattcaga tggtattttt
     2521 cttatagcag agaggatttt aagacagggg gtttttacag aactgatgcg gcatataaca
     2581 gatttaattc aggtgtttcg ctttcttctt caacttcaag ccctgttttc atccttcctt
     2641 caaagatcgt gaatatgaat cacagcatcc gttccggtct cggtctgagc ggaactgaca
     2701 tgaatgtgaa acaattgaaa gatccccttg acataactcc ttctgaaaag gacagattgg
     2761 atataaccgc ctatttttca tacggatttt cattctcttt caaccctgga tcatattatc
     2821 ttttcagcaa tatggaaagg acatcgtttt cttttccggt cacaatttac agatctttga
     2881 tgggtgatga caggtcgctt gtgttctccc cttcaatgca gttgagcttt ctcatccttc
     2941 caaacgggaa actcggattt atcacaagga atgtgcttta tatgagattc ctgaccgaca
     3001 actattttct tcttggcgga gacgaacttg acattgatat tctgaacctg aacacattca
     3061 tttacggcgg atcatcaagc gtaaccgtca gaggatacaa ttacggagcc gcaggaggaa
     3121 agcatgtcta ccactcaaac aacgaactga gattccacct tttctcgata agccagggtt
     3181 ttaacacatt tccactcatg ttcagaaatg ttcagggagc actgtttttt gacatcggaa
     3241 gcggctctcc cgacattaac atgtttgatg acaagttcat cgccgggatc ggagcagaac
     3301 taaaacttta ttcagtctgg tggtaccgcg tcccgataat atttacattc ggaaccgcct
     3361 acggattgac aaaaagcgga caactcaatt tctatttttc t
//
LOCUS       DAOOYB010000305         3398 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_33445_length_3398_cov_7.787616, whole genome shotgun sequence.
ACCESSION   DAOOYB010000305 DAOOYB010000000
VERSION     DAOOYB010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33445_length_3398_cov_7.787616"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1643
                     /locus_tag="PL056_12555"
     CDS             <1..1643
                     /locus_tag="PL056_12555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPM47894.1"
                     /translation="VAEGSNRFEWEHTRFDGSVFTAEIVLTPILEMNIETVFVIWRDI
                     SDKVKAKKAVEEAETKYKIITDNAPSAILIHQNMRWVYANKAVEEMFGIAREKLLGED
                     IFKYVHPSQRNQIIENAAKRIAGELIPERYDMCVSTPAEVEKWLDTKVSMITLDGDSA
                     ILVNCLDVTKRVEAERKLRESRERYKTTLNSIGDAVIATDALGNVQMMNPVAEQLTGW
                     KIGEAKGEPLETVFVISNAFTGEKVENPVTKVLKAGDIVGLANHTVLKSRNGNEYQIA
                     DSGSPIKDEFGNIRGVVLVFRDVTEKYVIEEQLRQTQKMEAIGQLASGLAHDFNNMLA
                     GIIGSAEIVADLIKGNSEAEAFTGRIKEAAMNANNLTRQLLDFSRKTKHEFVQINLSQ
                     LVSSAIDLVRSSIDPKITVTKLVDDEGLKLNGDKTKLQNALINLIFNARDAMPEGGVI
                     EISVTEHIIREHVVCRLLLPPGQYARISVKDTGTGIDEKIIGRIFDPYFTTKPAGKGT
                     GLGLAMVQKTVTAHKGAVTVTSKPGEGSVFEVYLPAEE"
     gene            1783..2481
                     /gene="pyrF"
                     /locus_tag="PL056_12560"
     CDS             1783..2481
                     /gene="pyrF"
                     /locus_tag="PL056_12560"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HPM47895.1"
                     /translation="MEKGKIFIALDVDTKKEALEIVNDLKGLGACFKIGKQLFTSLGP
                     DFVREIVGMGEDVFLDLKYHDIPNTVAKAGLAAASLGVKVFNVHAGGGRKMMEAVRAE
                     MNKLQNPPMILAVTVLTSMGAEDLAETGITVTPGQQVARLAKLALDSGMDGVVASPLE
                     IEIIRSVCGEDFKILTPGIRPASSSSDDQKRIATPSAALKAGSDYLVIGRPVTGAPDR
                     KKAFLDILAEIKAS"
     gene            2545..>3398
                     /gene="speD"
                     /locus_tag="PL056_12565"
     CDS             2545..>3398
                     /gene="speD"
                     /locus_tag="PL056_12565"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009353328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HPM47896.1"
                     /translation="MEVVLARSRKKSGSDNKLKLYGFNNLTKTLSFNIYDVCYAETPE
                     DRKAYIEYIDEEYNSDRLTGILEHVSDIIGSNVLNIAKQDYYPQGASVTVLISEEKVA
                     DPNCHEVHHEDAAENGGDVVMHMDKSHLTVHTYPESHPENGISTFRVDIDVSTCGKIS
                     PLKALNFLLDSFESDIVNLDYRVRGFTRRLDGSKIFIDHKLNSIQNFINMETLSRYHS
                     VDLNVYQDNIFHTKMILREFDLNNYLFGVEEAQLNTYKKRKIVKRVKKEMNEIFYGRN
                     IPSVPILY"
BASE COUNT         1075 a          663 c          807 g          853 t
ORIGIN      
        1 gtgttgcgga agggtcaaac aggtttgaat gggaacacac aagatttgac gggtcggttt
       61 ttacagcgga gatcgttctt accccgatac ttgaaatgaa tattgaaacg gtttttgtca
      121 tatggcgtga catttcagac aaagttaaag cgaaaaaagc ggttgaggaa gccgaaacaa
      181 aatacaaaat tatcacggac aatgctccgt ctgccatcct tatccatcag aatatgagat
      241 gggtttatgc caacaaggca gttgaagaga tgttcggcat tgcaagggaa aaattattag
      301 gtgaagacat cttcaaatat gtccatcctt ctcaaagaaa ccagataatt gaaaatgctg
      361 caaaaagaat tgccggtgaa ctaattccgg aaagatatga catgtgtgtc tcaactcctg
      421 ccgaagttga aaagtggctt gatactaaag tttcgatgat aactcttgac ggagatagcg
      481 ccattcttgt taactgcctt gatgtcacaa aaagggtgga ggcggaaagg aaactgcgcg
      541 aaagccgtga aagatataag acaactctca attcgatagg agatgcagta atcgcaactg
      601 atgcgcttgg caatgtccag atgatgaatc ctgtcgcgga acagcttacc ggctggaaga
      661 tcggggaagc aaaaggtgag ccgcttgaaa cggtttttgt catttcgaat gcctttacag
      721 gtgaaaaggt tgaaaatccg gtcaccaaag ttcttaaagc aggtgatatt gtcgggcttg
      781 cgaatcatac cgttctaaaa agcagaaacg ggaatgagta tcagatcgcc gacagcggtt
      841 ccccgataaa ggacgaattt ggaaatatca ggggtgtagt tcttgttttc agggatgtta
      901 cagaaaaata tgtgatcgag gaacagctca ggcagactca gaaaatggaa gcgatcggtc
      961 agcttgcgag cggtcttgca cacgatttca acaatatgct tgcagggatc atcggaagtg
     1021 cagagatcgt tgccgattta ataaaaggga acagtgaggc ggaagctttt accggcagga
     1081 taaaggaagc ggcaatgaat gcgaataatc tcacaagaca gctgctcgat ttttcaagaa
     1141 agacaaaaca cgaatttgtt cagatcaatt tgtcacagct tgtaagttca gctatcgacc
     1201 ttgtccgttc aagcattgat ccgaaaatta cagtaacaaa acttgttgat gatgagggtc
     1261 ttaagctcaa tggcgataaa accaagctgc agaacgcttt aataaatctc attttcaacg
     1321 caagagatgc catgccggaa gggggagtga tcgagatatc tgtgactgaa cacattatca
     1381 gggaacatgt tgtatgcaga cttcttctgc cgcccggaca gtatgcaagg atctcagtaa
     1441 aagacactgg aaccggaata gatgaaaaga taatcggaag gatattcgat ccctatttta
     1501 ccactaaacc ggcaggcaaa gggacaggac tcggtcttgc gatggttcag aaaacggtca
     1561 ctgcacacaa aggagccgta accgtgacat ctaagccggg agaaggaagc gtttttgaag
     1621 tttatctgcc tgcggaggaa tgatggaaat cccggcccgc tgggcacggg ttcagaaatc
     1681 gccacccttc aggcggcttg ttttgcgggc tgaaagctcg cgttatctta gcccacggct
     1741 gaaggcctgg gtgtgaatgt tatttgaatc tggagaataa tcatggaaaa agggaagatt
     1801 tttattgcac ttgatgttga cacgaaaaag gaagcacttg aaattgttaa tgacttgaaa
     1861 ggtttggggg catgttttaa gatcggcaaa cagcttttta cttctcttgg acctgatttt
     1921 gtcagagaaa ttgtcggtat gggtgaagat gtttttcttg acctgaaata tcacgatatt
     1981 ccaaacactg ttgcaaaagc cggtcttgcc gcggcatcac ttggagttaa ggttttcaat
     2041 gtccatgcag gcggcggaag aaaaatgatg gaagcggtga gagccgaaat gaacaaactt
     2101 caaaatcctc cgatgattct tgccgtcaca gttctgacaa gcatgggagc tgaagatctt
     2161 gctgaaacag ggataactgt gactcccggt cagcaggtgg caagattggc aaaacttgca
     2221 ctcgattcag gcatggacgg agttgtcgca agtccgcttg aaattgaaat aatcagatcg
     2281 gtatgcggag aagatttcaa aatactcacc cccggaataa ggcctgcatc aagttcaagt
     2341 gacgaccaga aaaggatagc cacaccatca gccgcactaa aggcgggaag cgactatctt
     2401 gttatcggaa gacctgtgac cggagctccc gacaggaaaa aagcatttct tgatatactc
     2461 gcagaaatca aagcatctta atttttttat tgaaaatgac atttttagag ttatattggc
     2521 ttccgttctt atcacctttg aatgatggag gttgttttgg caagaagcag aaaaaaaagc
     2581 ggatcagaca acaaacttaa actttacggg tttaataacc ttacaaaaac actgagtttt
     2641 aacatttatg atgtgtgcta tgccgaaacg cctgaagata gaaaggcata cattgaatat
     2701 attgacgagg aatataattc agaccgtctc acaggaattc tagaacatgt ttccgacata
     2761 atcggttcaa atgttctcaa tatcgcaaaa caggattact atccgcaggg tgcaagtgta
     2821 acagttctca tttcagaaga aaaggtcgca gatcccaact gtcatgaagt tcaccatgaa
     2881 gatgctgctg aaaacggtgg agatgttgtc atgcacatgg acaaatccca tcttacagtt
     2941 cacacatatc ctgaaagtca tcccgagaac ggcataagca ctttccgcgt tgacattgat
     3001 gtttcgacat gcggcaaaat ctctcctctg aaggcgctga attttcttct tgactcgttt
     3061 gaaagcgaca tcgtgaatct tgactaccgc gtcagaggtt tcacaagacg gcttgacgga
     3121 agcaagatat tcattgacca caagctcaat tccatccaga atttcatcaa tatggaaaca
     3181 ctttcaagat atcactcggt tgacctcaat gtttatcagg acaatatttt ccacacaaaa
     3241 atgatattgc gtgagtttga cctcaataac tatcttttcg gagttgaaga agcacagctt
     3301 aacacctata aaaaaagaaa gatcgttaaa agggtcaaaa aggaaatgaa cgaaatattc
     3361 tacggtcgta acatcccgag cgtcccgata ctttatta
//
LOCUS       DAOOYB010000306         3392 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_33505_length_3392_cov_9.722505, whole genome shotgun sequence.
ACCESSION   DAOOYB010000306 DAOOYB010000000
VERSION     DAOOYB010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33505_length_3392_cov_9.722505"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            64..>1750
                     /locus_tag="PL056_12570"
     CDS             64..>1750
                     /locus_tag="PL056_12570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47897.1"
                     /translation="MCFLFTVTLNDRILKTLFYAIPVLTGLVCLYINLFYIVTPQPDD
                     GIHYVWMAKMIANGKIFLELPDFYEHYWNLYVNIYDGKYVSIFLPGFSFFMAPFAALG
                     VPFLFNPLLAGLNAYLAGIHANALKDRKTAVIAMILFAFSSSHLLHGAQYFSHHFGLF
                     LVLVSSYIILHKSYSFKRFLLAGSILSISLMVRPQNACYTYFAVAVLILFKYRSFRPL
                     AGFTLPFIFTGSLLMGYNYYFTGDPFVFIQDIYFNAVNMRKFCHRPGFGKGCPGVQGT
                     FLPVEGLNFDYASDITFVRLNNFLFQITAHPALLLFIIPAVIKHPYKYFLYYFMPLCA
                     VVSYFPFYIEGSICGPRYLMESGALFLVVSSCGISQVIDYAARKKNVLGKLLIASCSG
                     LIISGAIFFSVMFVKKNLFENEKVYNLSKIIKIIEDNNIENSIVMLPFSYVFHFHSIL
                     NVQDEPPHDRFGNLIMYSMGEVDENIQKFYKDSRYSSIWKISEKDDEYLLQELDFLED
                     NGSYKVEFELKFIPSAGQPFFVLPMLENRDSSEFSYSQTENVRFSMNALGVLFR"
     assembly_gap    1751..1850
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2027..3346
                     /locus_tag="PL056_12575"
     CDS             2027..3346
                     /locus_tag="PL056_12575"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPM47898.1"
                     /translation="MKRKIEKELLKWMVRKNRKVLLIRGARQVGKTHLIREFGKKFSD
                     FVEINFDLHKKVHPFFDGDLDPVTIVKDLELYIGKKIIPGKTLLFFDEIQACKNAISS
                     LRYFYELMPNLHLIAAGSLLEFTLSEIPSFGVGRVESMFLFPLSFEEYLLELNGPDMV
                     EMIKNASFNKPVNTVIHEKLIDQLKIYQIIGGLPEVVQSYIDNRDLLDCQRMINVLIQ
                     GYYTDFIKYKDRSPVARLKETIESVAKQTTSKFKYSNIGSDSSNMYKESLEMLLKAGL
                     VYKAYHTNASGNPLGSGVNPKFFKAVMFDTGVYQRLMNLNLSDYVTTDFNTIINRGQL
                     TEIFAGSEIKKMKDPYSIDNLYYWHRESKSSQAEVDYVVEKNGKIIPVEVKAASGRKM
                     KSMHLFMEEKKSDFGIRLCADNFSKEGKIYSIPLYAAGFINDYEWLV"
BASE COUNT          982 a          555 c          647 g         1108 t
ORIGIN      
        1 ccatcttcaa tatttacagc ttttttccct gattttcata ttattttcat tatatgtgta
       61 ctgttgtgct ttcttttcac agttacatta aatgaccgta tcctgaaaac actcttttat
      121 gcaatccctg ttttaacggg tctggtttgt ctttatatca acctttttta tattgtgacg
      181 cctcagcccg atgacggaat ccattatgtc tggatggcaa agatgatcgc aaacgggaaa
      241 atattccttg aactgcctga tttttatgaa cattactgga atctttatgt gaatatctac
      301 gacgggaaat atgtctccat tttccttcct ggtttcagtt ttttcatggc cccttttgct
      361 gcactcggcg ttccttttct gtttaatccg ctgcttgccg gcttgaacgc atatcttgca
      421 ggaattcatg ctaatgcact gaaagaccgc aaaacagcgg tgatcgcaat gatccttttt
      481 gcattctcat caagccatct gcttcacggt gcacagtatt tttcacacca tttcggactg
      541 tttcttgtcc ttgtttcaag ttatattatt cttcataaaa gttacagctt caagagattc
      601 cttcttgccg gatcaattct ttcgatatcg ctcatggtca gaccgcagaa tgcctgctac
      661 acttattttg ccgttgcggt tctcatttta tttaaataca gatctttcag accgcttgcc
      721 ggttttactc tgccgtttat tttcaccggc tctcttttaa tgggatacaa ctattatttt
      781 acaggtgatc cgtttgtttt tattcaggat atttatttca atgcggtcaa tatgagaaag
      841 ttctgccaca gaccgggatt tggaaaaggg tgtcccggtg ttcagggaac atttcttcca
      901 gtagaagggt tgaattttga ttatgcgtca gatataactt ttgtaagact gaacaacttt
      961 cttttccaga taacggctca tccggcactg cttcttttta taattcctgc cgtaataaaa
     1021 catccttata aatattttct ttattatttc atgccgttat gtgctgttgt ctcatatttt
     1081 ccgttttata ttgaaggaag catttgcgga ccccgttatc tcatggaatc gggagcactt
     1141 tttcttgtgg tttcgtcatg tggaatttca caagttattg attatgcggc cagaaaaaag
     1201 aatgttctgg gaaaattgtt gatcgcctca tgttcagggc tgataatttc aggggctatc
     1261 tttttttcag tgatgtttgt gaaaaagaat ctttttgaaa atgagaaggt gtataacttg
     1321 agtaaaatca ttaagataat agaggataac aatatcgaga attcaatagt gatgctgccg
     1381 ttttcatatg tgttccattt tcattcgata ttaaatgttc aggacgagcc gccacatgat
     1441 agattcggca atctgataat gtattcaatg ggcgaagtgg atgaaaacat tcagaaattt
     1501 tataaggatt caagatacag ttcaatatgg aaaatatctg agaaagatga tgaatatttg
     1561 ttgcaggagc ttgattttct tgaagataac ggatcgtata aggtggaatt tgaactgaaa
     1621 ttcatccctt cagccggaca gccgttcttt gtcctgccga tgcttgaaaa ccgcgattct
     1681 tccgagtttt catattcgca aacagaaaat gtcagattca gcatgaatgc tttgggagtt
     1741 ctgtttagag nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gttatagacg
     1861 gatgcatagt tttagattat atgaccatta atcaacgcga ttaatagaac agtcgaattc
     1921 tgttgttaac cttgtatttt tgaaataaaa gtgccaaagt aaccttgtat ttttgaaata
     1981 aaagtgctaa agtaaccttg tatttttgaa atatgagggg cgttcaatga agagaaaaat
     2041 tgaaaaagag ttgttaaaat ggatggttag aaaaaacaga aaggttcttc tcatcagagg
     2101 ggcaagacag gtcgggaaaa cgcatttgat aagagagttc ggaaagaaat tttcagactt
     2161 tgtggagata aattttgatc ttcataaaaa agtacatcct ttttttgatg gtgatcttga
     2221 tccggttaca attgtaaaag atctggaact ttatatcgga aagaaaatta taccgggcaa
     2281 aactcttttg ttctttgatg aaattcaggc atgtaaaaac gcaataagtt cgttgcgtta
     2341 tttttatgaa ttaatgccga atcttcactt gattgctgct ggatctcttc ttgaatttac
     2401 cttgtcagaa attccatcat tcggtgtcgg aagggtagaa tcaatgttcc tttttccttt
     2461 aagttttgaa gaatatcttc ttgaattgaa tggtccggat atggttgaga tgataaaaaa
     2521 cgcttcattt aacaagccgg tgaatactgt aattcatgaa aagctgattg atcagttaaa
     2581 aatatatcag ataattggtg gacttcccga agttgttcaa agttatattg acaacagaga
     2641 tcttcttgac tgtcagagaa tgatcaatgt tctcattcag ggttattata ctgattttat
     2701 taaatataag gaccgatctc cggttgcaag actgaaagaa acgattgaat ctgtcgcaaa
     2761 acagactact tcaaagttca aatacagcaa catcgggagt gattcttcaa atatgtataa
     2821 agagtctctt gaaatgttat taaaagcggg acttgtttat aaggcatatc atacaaacgc
     2881 atcgggaaat ccactgggaa gtggagtgaa tcctaagttt ttcaaagcag ttatgtttga
     2941 cacaggagtt tatcagagat taatgaatct taatctttca gattatgtca ctactgattt
     3001 caatacaata ataaatcggg gacaactcac cgagatattt gccgggtcag aaataaaaaa
     3061 gatgaaagac ccttacagta tcgacaatct ttattattgg cacagagaat caaaatcaag
     3121 tcaggcggaa gttgactatg ttgttgagaa aaatgggaaa ataatccctg ttgaagtaaa
     3181 agcagcatcc ggcaggaaaa tgaaaagcat gcatcttttt atggaggaaa agaaatctga
     3241 ttttggcata agactatgtg cagacaactt ctccaaagaa ggaaaaatct attccattcc
     3301 actctatgct gctggattta ttaatgatta tgaatggctg gtgtaagggg ttttaaagat
     3361 ctttttccct aaaaagtgat cactgaacag cc
//
LOCUS       DAOOYB010000307         3340 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_34221_length_3340_cov_12.926027, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000307 DAOOYB010000000
VERSION     DAOOYB010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3340
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34221_length_3340_cov_12.926027"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="PL056_12580"
     CDS             complement(<1..347)
                     /locus_tag="PL056_12580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47899.1"
                     /translation="MKKVIIFTALFFVLFSINAATLTVCETGCDYTSVKTAVGAAAAD
                     DTIQISAGIYLDWNITISKNLTLIGAGRHDTIVQAATKEAGGSYRVFKVTAGTVTFKN
                     MTIRYGYSDNDGY"
     gene            496..2472
                     /gene="ligA"
                     /locus_tag="PL056_12585"
     CDS             496..2472
                     /gene="ligA"
                     /locus_tag="PL056_12585"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HPM47900.1"
                     /translation="MKERYLELVNLLNRASAAYYRDDSPFMSDSEYDRLYRELIELED
                     KYPEIKVKHSPSMRVGAEIREGFKKVAHSEKMMSLDDAFDVMEVEQFFNRLDFEPGNG
                     FVVEQKIDGLAVNILYNFGKFVQAATRGDGMIGEDVTQNVATVKDIPLEIQELSDVRN
                     IEVRGEIYMPLSSFEELNKLKLESDEKPFANPRNAAAGSLRQLDPKITAQRKLKFFAY
                     GITGIAEGTVETQERLHKFLRAVGFKTPDFIKIEKIEQIEDLVKEIISERAGLPYDID
                     GLVVKLNSFEDQKIAGFLTKTPKWAIAYKLPAVEKTTKLLDITWQVGRTGVVTPVAEL
                     EPVEIGGVVVKRATLHNIDEIRRKGLMIGDTVFVRRAGDVIPEVIAPVEHFRNGNELT
                     ALAPCSCPVCNTDLIKDEDKVALVCPNSGCPSKVAGSIEHFVSRKGFNIDGLGEKQIE
                     FVYKKGWVKTVADIFSLSEYRNELSIEKGWGEKSVTKLFEAINKSKNIKFENFLYALG
                     IDGVGEFLAGELAKNFTIAELMTAETGMLMAIEGIGETVAKAIKEYFTNYDNRVIVER
                     LINETVISYPEKSDLTNKILSGKTFVITGELSEPREMFAELIKNLGGKVSSSVSKKTH
                     YLLAGENAGSKLAKATELNVKVINEEEFKQLIEK"
     gene            2524..3273
                     /locus_tag="PL056_12590"
     CDS             2524..3273
                     /locus_tag="PL056_12590"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HPM47901.1"
                     /translation="MMKKLAFVMVMLLTFCIMAQDKTVTDVKEILDKMDKVYRADSSE
                     FEMEMEIVTPDWKRTMKMKAWSEGMKKTFITILSPSKDKGIGTLKVDSDMWNYFPKVN
                     KVMKVPPSMMMGSWMGSDFTNDDLVKETTFRDDHTAKLLESTEKDIIVIELKPKESAA
                     SVWGKIIAKVRKSDYLPVSEDYYDEKGMKVRTMTFSGIKEMGGKIIPSVMEIIPLNKK
                     GNSTKITYLNGKFDIKLDPETFSLRNLQKMR"
BASE COUNT         1119 a          573 c          764 g          884 t
ORIGIN      
        1 ttataaccgt cgttgtctga gtaaccatat ctgatcgtca tatttttaaa tgttacggtt
       61 ccggcagtta ctttgaaaac tcgatagctt ccgcctgctt cttttgtcgc cgcctgaact
      121 atcgtatcgt gtcttcctgc accgatcaga gtaagatttt ttgaaattgt aatattccag
      181 tcaagataaa ttcctgctga aatctggatc gtatcatccg cagcggcggc gccgaccgca
      241 gttttgactg aagtgtagtc gcaacccgtt tcacaaacgg ttaatgttgc cgcattaatt
      301 gaaaacagaa caaaaaacag agccgtgaaa atgataactt ttttcatgat accctcctca
      361 aagaaataaa aaagcacttg aacccaaagt aaggatataa agaaaaaagt caaaaaagca
      421 gattgcccca ttttatttgc caatttgacg aatctcaaat atatttagat gtttttgata
      481 attcaggatg tgagcatgaa ggaaaggtat cttgagcttg taaatcttct taacagggca
      541 tctgcggcat attaccgtga tgattctcct tttatgagcg attcagagta tgaccggctt
      601 tatcgcgagc ttattgaact tgaggataag tatccggaaa taaaggtgaa acactctcca
      661 tcaatgaggg ttggggcaga gatcagagag ggttttaaaa aggttgcaca ctctgaaaaa
      721 atgatgtcgc ttgatgatgc attcgatgtt atggaggtcg agcagttttt caatcggctt
      781 gatttcgaac ccgggaacgg ttttgtcgtt gagcagaaaa ttgacgggct tgcggtcaac
      841 attctttaca atttcgggaa atttgttcag gcggcaaccc gcggggatgg aatgataggg
      901 gaggatgtga cacagaatgt tgcaactgtc aaagatattc ctcttgaaat tcaggagctt
      961 agcgatgtgc ggaatatcga ggtgagaggc gagatatata tgccgctgtc aagttttgaa
     1021 gagcttaaca aattaaaact tgaaagtgac gaaaagccgt ttgccaaccc gagaaatgcg
     1081 gcggccggct cattaagaca gcttgatccg aagatcactg ctcaaagaaa actcaaattt
     1141 tttgcatacg gaataacggg tatcgcagaa ggaacagtgg aaactcagga acggcttcat
     1201 aaatttttaa gggctgtggg tttcaaaacg cctgatttta taaaaatcga aaagattgaa
     1261 caaattgaag atcttgtgaa agaaattata agcgaaagag cgggacttcc atacgatatt
     1321 gacggacttg ttgttaagct caacagcttt gaagatcaaa aaattgcggg atttctgaca
     1381 aaaacgccga aatgggctat tgcatataaa cttcctgccg ttgaaaagac cacaaaactt
     1441 cttgatataa cctggcaggt ggggagaacc ggagttgtca caccggttgc ggagcttgaa
     1501 ccggttgaga tcggaggagt tgtcgtaaaa agggcgactc tgcacaacat tgacgaaatc
     1561 agaagaaaag ggctgatgat cggtgacaca gtttttgtaa gaagggcagg agatgtcatc
     1621 cctgaagtca tcgctcctgt tgaacatttc agaaacggca atgaactcac ggctcttgct
     1681 ccatgttcat gtccagtctg caatactgac cttataaaag acgaagacaa ggtcgcactg
     1741 gtatgtccca attcgggctg tccttcaaaa gtggctgggt ccattgaaca ttttgtttca
     1801 agaaagggtt ttaacattga tggacttggc gaaaaacaga tagaattcgt ctataaaaaa
     1861 gggtgggtta aaactgttgc cgacattttt tcactttctg aatacaggaa tgaactttca
     1921 attgaaaaag ggtggggtga aaaaagtgtc accaagcttt ttgaggcaat taataagtca
     1981 aaaaacataa aatttgaaaa ttttctgtat gctctcggaa ttgacggagt gggggaattc
     2041 cttgccggag aacttgcaaa aaactttaca attgctgaac ttatgaccgc tgaaacagga
     2101 atgctcatgg caattgaagg gattggtgaa actgttgcga aagctataaa agagtatttt
     2161 acaaattatg ataatcgtgt aattgtcgaa cggctcatta acgaaacagt tatttcatac
     2221 cctgaaaaaa gtgacctcac aaataaaatt ttatcaggca aaacatttgt aatcactgga
     2281 gaattgtctg aaccaagaga aatgttcgct gaacttatca aaaatcttgg aggcaaagtc
     2341 tcatcatcag tcagcaaaaa gacacattat ctccttgccg gagaaaatgc cggaagcaag
     2401 ttggcaaaag cgaccgaact taatgtcaaa gtaataaacg aagaagaatt caaacaactt
     2461 atagaaaaat aaaaaagatt tttttttgac tgaacagttg gtcttatgga aatggaggga
     2521 agaatgatga aaaaactcgc ttttgtgatg gtgatgctgc tcactttctg catcatggcg
     2581 caagataaga cagttacgga tgtcaaagag atccttgata aaatggataa ggtttaccgg
     2641 gcagattctt cagaatttga aatggaaatg gagatcgtca ctcctgactg gaaaaggacg
     2701 atgaagatga aagcttggag cgaagggatg aaaaagacat tcataaccat actttctcct
     2761 tcaaaagata aagggattgg cactttgaaa gtggatagcg atatgtggaa ctattttccc
     2821 aaagtgaaca aagttatgaa agttccgcca agcatgatga tgggaagctg gatgggaagt
     2881 gatttcacga acgatgattt agttaaagag acaactttcc gtgacgatca tactgcaaag
     2941 ctgctcgaaa gcacagaaaa ggatattatt gtgatcgaac tcaagccaaa agaaagtgcc
     3001 gcctcagtct gggggaagat aattgcaaaa gtaagaaaaa gcgattatct gccggtttcg
     3061 gaggattatt atgatgaaaa agggatgaaa gtcagaacaa tgactttttc aggcataaaa
     3121 gagatgggtg gaaaaataat tccttctgtt atggagatca ttcctttaaa taaaaagggg
     3181 aattcaacaa agatcacata tctaaacgga aaatttgata ttaagcttga ccccgagacc
     3241 ttttcactta gaaatcttca gaaaatgagg taggcatgat cattttaaaa atggcattcc
     3301 ggaacattct caagcagaaa agaaggacat tttttacact
//
LOCUS       DAOOYB010000308         3335 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_34323_length_3335_cov_5.782317, whole genome shotgun sequence.
ACCESSION   DAOOYB010000308 DAOOYB010000000
VERSION     DAOOYB010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34323_length_3335_cov_5.782317"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1153)
                     /locus_tag="PL056_12595"
     CDS             complement(<1..1153)
                     /locus_tag="PL056_12595"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HPM47902.1"
                     /translation="MRTEELKRLLSLGENEKVEFKRQYNSDSAGREICAFLNSKGGFL
                     ICGVEDDKGTVAGVNLNLSKVENELAEHIIPKAPFFFELHDVEGKKVLVIEVPAGKDI
                     PYSFNNDIFIRVRDKTKKADIETIREMVFRRQNEPERWERRFSDAQIDQDVDYDHLQS
                     IGNKIKKSGRYSFAVNKNKETILESLSLEKFGRLTNAGDVLLCTNTANRYPQVIVKAA
                     SFPTGKTSDEFRDFKNFDGPLLKVLEEVYNFILRNTSTRSYFIKDSLERKDEPVYPMG
                     AVREGLINAFVHRDYSDYQGSLSVFIYPDRLEISNSGEFPKGITADKIGYGQISVLRN
                     PDIANMVYICGMMEKVGRGGLFIRESCKEKGLPEPLWTNKKDLGVTLTLF"
     gene            complement(1150..1755)
                     /locus_tag="PL056_12600"
     CDS             complement(1150..1755)
                     /locus_tag="PL056_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011920343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HPM47903.1"
                     /translation="MAKKKTIMVSSTVYGIEELLEKIYSLLTSFGYEVWMSHKGTIPV
                     LSKKTAFENCLDAVEKCDLFLGIITPFYGSGTDEKDAISITHMELRKAVELNKLRWLL
                     SHDHVVFARNFLKNLGYDTSEKRKALKYKKSDVFGDLRVIDMYEEAILSQQPLRDRKG
                     NWVQKFDRDEDAQLFTTAQFSRFQEAEEFINDNIKKKGGSK"
     gene            complement(1757..3283)
                     /locus_tag="PL056_12605"
     CDS             complement(1757..3283)
                     /locus_tag="PL056_12605"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HPM47904.1"
                     /translation="MAKEKIVTTEPIEKQLWKTADKLRKNIDAAEYKHIVLGLIFLKY
                     ISDAFEEMYAKLKAQERDGADPEDKDEYKAENVFFVPPSARWHFLQSHAKQPTIGKTV
                     DESMDAIEKENPSLKDVLPKVYARGNLDPVSLGGLIDLISNVALGDAKARSADLLGHV
                     FEYFLCEFALAEGKKGGQFYTPRSVVELLVEMLQPYKGRVMDPCCGSGGMFVQSEKFV
                     AGHQGRVDDISIFGQESNQTTWRLAKMNLAIRGIDSSQVRWNNEGSFLNDAHKDIKVD
                     FVIANPPFNDSDWSGDLLRNDARWKYGTPPSGNANYAWIQHFLYHLAPGGIAGFVLAK
                     GSLTSKSSGEGDIRKALIEANLVDCVVNLPAKLFLNTQIPASLWFLRRNKTTRVNEIL
                     FIDARNEGHLINRRTKEFSSDDIKKIADTYHNWRNPNGNYEDIKGFCNSTSIERVKEL
                     DFVLTPGRYVGLPDEEDDFNFAERFTTLKAEFEEQLKEESKLNEAILENLKKVKIEEK
                     "
BASE COUNT          920 a          759 c          541 g         1115 t
ORIGIN      
        1 caaaaagtgt taaagtaaca ccgagatctt ttttatttgt ccacaatggc tcaggcagac
       61 ctttttcctt acatgattca cggataaata gtccgcctct ccctactttt tccatcatgc
      121 cgcatatata aaccatattt gctatatccg gatttcgcaa tactgatatt tgtccgtaac
      181 cgattttatc cgctgtaata cctttaggaa attctcctga atttgatatt tcaagacggt
      241 ccggataaat aaaaacagaa agacttcctt gataatcaga gtaatcacgg tgaacaaatg
      301 catttatcag tccttctctt actgcaccca taggatagac aggttcatct tttctttcca
      361 gagaatcttt aataaaataa gatcttgtcg aagtatttct gagaataaag ttatatacct
      421 cttccagaac ttttaaaagc ggtccgtcaa agtttttgaa atctctgaat tcatcgcttg
      481 tctttcctgt gggaaaactt gccgccttca ctatcacctg cggatatctg ttcgccgtgt
      541 tggtacataa aagaacatcc cctgcattcg taagtctgcc gaatttctca agtgaaagag
      601 attcaagaat agtttcctta tttttattca cagcaaaact atatcttccg gattttttta
      661 ttttatttcc aattgactgg agatggtcat aatcgacatc ctgatcaatc tgagcatcag
      721 aaaaacggcg ttcccacctt tcaggctcat tctgtctgcg gaaaaccatt tcacgaattg
      781 tttcaatatc agcttttttt gttttatccc tgactcttat aaatatgtcg ttattaaaag
      841 agtatggaat gtcttttccg gcaggaactt caatgacaag aacttttttt ccttcaacat
      901 catgtaactc aaagaaaaaa ggagctttcg gaataatgtg ttctgccagt tcattttcaa
      961 ctttgctcaa attcaaatta actccggcaa cagtcccttt atcgtcttca accccacaga
     1021 tcagaaaccc gccctttgaa tttagaaatg cacaaatctc tcttcctgct gagtcagaat
     1081 tatattgtct tttaaactca actttttcat tttcgccaag agaaagcagt ctttttaatt
     1141 cttctgttct catttactgc cccccttttt tttaatatta tcatttataa actcttccgc
     1201 ctcctgaaaa cgggaaaact gtgctgtagt aaagagttgt gcgtcttcat ccctgtcaaa
     1261 tttctgaacc cagtttcctt ttctgtcacg aagaggttgc tgcgaaagta ttgcttcttc
     1321 atacatatca atcaccctca agtcaccaaa cacatcactt tttttatatt taagtgcttt
     1381 tctcttttct gatgtgtcat atcccagatt tttcaagaaa ttgcgtgcaa aaacaacatg
     1441 atcatgagaa agaagccagc gaagtttatt taattcaacg gcttttctaa gttccatgtg
     1501 agtgatggaa atagcatctt tttcatctgt tccgctaccg taaaaaggtg taattatgcc
     1561 aagaaaaaga tcacattttt caacagcgtc aagacaattt tcaaatgctg tcttttttga
     1621 caaaacagga attgttccct tgtgtgacat ccagacctca tatccaaatg aagtaagaag
     1681 cgagtatatt ttttcaagaa gctcttctat cccgtaaaca gttgaagaga ccattatcgt
     1741 tttctttttc gccattttat ttctcctcta tcttaacctt cttcagattc tcaaggatcg
     1801 cttcattaag ttttgattct tctttgagct gttcttcaaa ttcggctttg agggttgtaa
     1861 atctttcagc aaaattgaag tcatcttctt catcggggag tccgacatat ctgcccggtg
     1921 tgagaacaaa atcaagttct ttcactcttt caatgcttgt tgaattgcag aatcctttga
     1981 tatcttcata gttaccgttc ggatttctcc aattgtggta tgtatctgcg atctttttta
     2041 tgtcatcaga cgaaaattct tttgttcttc tgtttatgag atgtccttcg ttccttgcat
     2101 caatgaagag tatttcattc actcttgttg ttttgttcct tcttaaaaac cagagcgatg
     2161 ccgggatctg ggtattaagg aaaagttttg caggaagatt cacgacacag tcaacaaggt
     2221 tcgcttcgat aagtgctttt ctgatgtcac cttcacctga actttttgaa gtgagcgatc
     2281 cttttgcaag tacaaacccg gcaattccgc ccggtgcaag atgataaaga aaatgctgaa
     2341 tccacgcata attagcattt ccggatggag gcgttccata tttccacctt gcatcattcc
     2401 ttaaaagatc tccgctccag tcgctatcat taaaaggagg atttgcaatg acaaaatcaa
     2461 cctttatatc tttgtgagca tcgtttagaa aagatccttc gttattccat ctgacctgtg
     2521 aactgtcaat tccccggata gcaagattca tttttgcaag ccgccaggtc gtctgattgc
     2581 tttcctgacc gaaaatcgaa atatcatcga ctcttccctg atgaccggca acaaactttt
     2641 cagactggac aaacattccg ccgcttccgc aacacgggtc cataactctt cctttatacg
     2701 gctgaagcat ttctacaaga agctctacaa cacttcttgg ggtatagaac tgtccccctt
     2761 tttttccttc agcaagagca aattcacaaa gaaaatattc aaaaacatga cccagaagat
     2821 ccgcacttct tgcctttgca tcaccgagag ccacattgct gataaggtcg ataagaccgc
     2881 caagactcac cggatcgaga ttcccacggg cataaacttt cggaagaaca tctttaagtg
     2941 acggattttc cttttctatc gcatccattg attcatcaac ggtttttcca atcgtcggct
     3001 gctttgcatg ggattgaaga aaatgccacc ttgcactcgg cggaacaaag aaaacattct
     3061 ccgccttgta ttcatcttta tcttccggat cagcaccatc ccgttcctga gctttgagct
     3121 ttgcatacat ctcttcaaaa gcatccgaaa tatatttcag gaatatcaac cccagaacta
     3181 tgtgcttata ttcagccgca tcaatattct ttctgagctt atccgccgtt ttccaaagct
     3241 gtttttcaat aggttcagtt gtaacgattt tctcttttgc cattgtccac tcctgcattt
     3301 tccaattcca atcccgatta ttctaaactt taaga
//
LOCUS       DAOOYB010000309         3326 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_34452_length_3326_cov_11.926628, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000309 DAOOYB010000000
VERSION     DAOOYB010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3326
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34452_length_3326_cov_11.926628"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            27..1919
                     /locus_tag="PL056_12610"
     CDS             27..1919
                     /locus_tag="PL056_12610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47905.1"
                     /translation="MLYIGKKREMAEYFKSIPLEKKQQLYRGVRSIMAMLVKISKMTE
                     IYSVRDKIFDEFTETIKQNFDVIHNALPYATVTTKKVGFYYQDKKIKLFDEGERKFRN
                     IFLVNEIRELYFVQGVTPQEIIDFFAVIQETLNYTLLDYDFNTRLWDYGINHIGTISD
                     PDMGDPEPWCDDGFKSEFEPVKPEVLFSCRPRSFHELAQIVPEFDNIGKTRSSEFTEW
                     VEKSGKELTVKKYLDKARALISSGLNKENNRNIVNKVCEYAIRNIQNGDFISGIVYVN
                     TLIQLARELSNTDEVLFMRVKEVLKRINAEEFVNKMFEMAGKMDSSQTKSFGDLVNLV
                     SSTNFELVFLKLCELENKDARMYCLEGIAKNFKDAELAKKFVKHEDWHIARNFLYMLR
                     FAFNPEFLPSVRDVMNHQVKQIRVEAARVLSLYDADENLEYWQKAVFSPDEEVRILAV
                     ENLVRVKGLEAKAIFNEIFKPSNRNRFNLTDYERYIDRILASQRKEFFDLPGTMIFSE
                     NLELRLTVLKSLNKIDDPGIIATQIIRRMKSPEFLELGENEILLLLGLVKGSSVTSML
                     EEMEYLFTLQGGFLNKKKYAPVKKIVFDYFRSVGQKNQIIKRWLEKGLKTGNDETKAI
                     LQGREL"
     gene            1920..3326
                     /locus_tag="PL056_12615"
     CDS             1920..3326
                     /locus_tag="PL056_12615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47906.1"
                     /translation="MEERRNKPEFEKSPKKWRYNFSKSLFTFWKTVKMYGGENERIDE
                     EINFFRKVLDFFFFEKDEVSILFDGIDIKIDKVRIRGQRQDDKYFEDIYDLFLSLCLA
                     GITFKKGVTDKEILELFRVIGKYPVGREPKVQAFERVKGDLPRLDNIEIFPYDPEESG
                     NLPIYSVSEKIRKIYRTLCSEYLEYKKMVDAVENIPLRIIERNVQDLISIVKNSEDPN
                     EMNMLLFLSSVNAYKGSFKGASAAARVILSTMIALKLDFDIHTVKRIAISAYFQYFAD
                     DLTKGYTALSRMDDFNYSRIEAALNSSLRITDFSDEGIMRGNSTSGTVSGDILKVVSY
                     FDSVTKKWPERVGYKGPLLSRHEAVRSILRNVKKGAFRREIAEALVGVAGIYPPGNIM
                     KIISTNEFCISGGRFRSFSDEAPVFILNPDLSLKEMKIVKAENLVDVPDAAGLILPPS
                     LYVSMLKVFLDDSEESAG"
BASE COUNT         1085 a          555 c          755 g          931 t
ORIGIN      
        1 aataaatttc tctgtcggag gttgatttgc tgtacatcgg gaaaaaaagg gagatggcgg
       61 aatacttcaa atcaattccg cttgaaaaga aacagcagtt gtacagagga gtcaggtcga
      121 taatggcaat gcttgtcaaa atatcgaaaa tgaccgagat ctattctgtc agggacaaga
      181 tatttgatga gtttacagaa accattaaac agaacttcga tgtcattcat aatgctctgc
      241 cctatgcaac tgtaacaacc aaaaaggtcg gattctatta tcaggacaag aaaataaagc
      301 tttttgatga aggtgaaaga aagttcagga acatctttct tgtgaacgaa attagggaac
      361 tctattttgt tcagggcgtc actccgcagg agatcattga tttctttgcc gtcattcagg
      421 agacactgaa ttatacactg cttgattatg attttaatac gcggctgtgg gattacggta
      481 tcaatcacat cggaacgatc tccgatcctg acatgggtga tcctgagccc tggtgtgatg
      541 acgggtttaa gtccgaattc gagccggtaa agccggaagt gcttttttcg tgcagaccga
      601 gaagttttca tgaacttgcc cagatagttc ctgagtttga caacatcgga aagaccagaa
      661 gcagtgagtt tactgaatgg gttgaaaaga gcgggaagga attgaccgta aagaaatatc
      721 ttgataaagc cagagcactt atatcatccg ggcttaataa ggaaaacaac aggaacattg
      781 tcaataaggt gtgtgaatat gccataagaa atatccagaa cggtgatttt atttccggca
      841 tagtctatgt taacacgctt attcagcttg ccagggaact gtcaaatacc gacgaagttc
      901 ttttcatgag agtcaaagaa gttcttaaaa ggataaatgc cgaagagttt gttaataaaa
      961 tgtttgaaat ggcagggaaa atggactctt cccagacaaa aagtttcgga gatcttgtca
     1021 accttgtaag ttcaactaat tttgaacttg ttttccttaa actttgtgaa cttgagaata
     1081 aagatgcaag aatgtactgt cttgaaggga tagcaaaaaa cttcaaggat gccgagttgg
     1141 caaagaaatt tgtaaaacat gaagactggc atatcgcacg gaactttctt tatatgctga
     1201 gatttgcatt caatcccgaa ttccttcctt ctgtcagaga tgttatgaac catcaggtga
     1261 aacagatacg ggtcgaagcg gcaagagttc tgagcttgta tgatgccgat gagaatcttg
     1321 agtattggca gaaagccgtt ttcagtccgg atgaagaggt caggatcctg gcagttgaaa
     1381 atcttgtcag agtaaaaggg ttggaggcaa aagcgatatt caatgaaatt ttcaagccat
     1441 ccaacagaaa caggttcaat cttactgact atgaaaggta tattgacaga attcttgctt
     1501 cccagagaaa agaatttttc gatctcccgg gaaccatgat cttttctgaa aatcttgagt
     1561 tgcgtcttac ggtgctcaag tcacttaata aaattgatga tccggggatt attgcaaccc
     1621 agataataag aagaatgaaa agccctgaat ttcttgaact cggagaaaat gaaattcttc
     1681 tccttctcgg acttgtaaaa ggaagttctg tcacgagtat gcttgaagag atggaatacc
     1741 ttttcactct tcagggcgga ttcctcaata aaaagaaata tgctccggta aagaaaatag
     1801 tatttgatta tttcagatcg gtcggacaga aaaatcagat aataaaaaga tggctcgaaa
     1861 agggtttgaa gaccggcaat gatgaaacta aagccattct tcaggggagg gaattataga
     1921 tggaggaaag aaggaacaaa ccggaatttg agaaatctcc taaaaagtgg cgttacaatt
     1981 tttccaaatc attgtttaca ttctggaaaa ctgtaaaaat gtatggcggt gaaaatgaaa
     2041 ggatagatga agagataaat tttttccgca aggttcttga cttcttcttt tttgaaaagg
     2101 atgaggtttc aatacttttt gacggaattg acataaaaat tgataaagtc aggatcaggg
     2161 gtcaaagaca ggacgacaaa tactttgagg atatttacga tctttttctt tctctctgcc
     2221 ttgcaggtat aacatttaag aaaggggtca cagataaaga gatccttgaa ctcttcaggg
     2281 taataggaaa atatcctgtc gggagagaac cgaaagtaca ggcattcgag agggtgaagg
     2341 gagatcttcc tcggttagat aacatagaga tatttccata cgatcctgaa gagtcgggaa
     2401 atcttcccat ttacagcgtg tcggagaaga tcaggaagat atacagaacc ctttgttcag
     2461 aatatcttga atataagaaa atggtcgatg cagttgagaa cataccgttg agaataattg
     2521 aaagaaatgt acaggatctt atttccatag tgaagaacag cgaagatccc aatgaaatga
     2581 acatgcttct ttttctttca tcagtaaatg cgtataaagg ttcattcaaa ggcgcttcgg
     2641 ctgccgcaag ggtgattctt tcaactatga tcgccctgaa gcttgatttt gatatacaca
     2701 ctgttaaaag aatagcgatc tcagcatatt tccagtattt tgcagatgat cttacaaaag
     2761 gttatacggc actttcaaga atggatgatt ttaattacag cagaattgaa gccgcattga
     2821 attcttcttt gagaataaca gatttcagcg atgaagggat aatgaggggc aacagcactt
     2881 caggaacagt atcgggagat attttaaaag ttgtgagcta ttttgacagt gtaaccaaaa
     2941 aatggcctga aagggttgga tataaagggc cccttctttc taggcatgaa gctgtgagaa
     3001 gtattttgag aaatgttaaa aaaggtgcat tcagaagaga gatagctgaa gcgcttgttg
     3061 gtgttgccgg aatctatcct cccggaaata ttatgaaaat catttccaca aacgaattct
     3121 gcatatcagg aggaagattt cgaagtttca gtgatgaagc gccggtcttt attctcaacc
     3181 ccgacctttc tttaaaagag atgaagatcg ttaaggccga aaatcttgtt gatgttcctg
     3241 atgccgccgg attgatcctt cctccttcct tgtatgtaag catgttaaaa gtttttctgg
     3301 atgattcgga agagtccgca ggatag
//
LOCUS       DAOOYB010000310         3324 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_34492_length_3324_cov_6.651270, whole genome shotgun sequence.
ACCESSION   DAOOYB010000310 DAOOYB010000000
VERSION     DAOOYB010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3324
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34492_length_3324_cov_6.651270"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            300..857
                     /locus_tag="PL056_12620"
     CDS             300..857
                     /locus_tag="PL056_12620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47907.1"
                     /translation="MFAGLFGEGKIVIDEKTAQEIEKLKSALKSVGVEYELGAFIPES
                     DENGVEDALTVDEFIESLESMSVRSTAYISAYALIPLYGWYLVNGHWVIEWTGKVSSS
                     PDIEGDDWDGVMHTRETSGGTVVETKITFNPTYAYLWDAVYESTPRLCAQLFNNVRVY
                     VPGIGYILVPQVFGSKHCGTVNFNW"
     gene            912..>3324
                     /locus_tag="PL056_12625"
     CDS             912..>3324
                     /locus_tag="PL056_12625"
                     /inference="COORDINATES: protein motif:HMM:NF012632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47908.1"
                     /translation="MKTVFILIVIFIASCSGSPSKNDEDILVDNDTNINDEEASDEDI
                     EHTPACINEWEKTEIVEDVSELFDFDVKVETGKWKWEKKDRHEELVYGADPSMPLGVM
                     NDGSLVLGESFETENGHSIVSILNPDGTFKRYVLEIEKGNEINYGQGELSANGFEPFN
                     FWPGYSFDEERQEILIPVTIRSKIKIPGSEDEYFGRPFLIKIDAQGNLSYIAWKNNGN
                     SVCNHLVQTDDKIIMMCLHWSSDLDIYVATGKINAEINLIYKGNVYRKLMQSDSYASM
                     GPFLSYENGKVYAHYREEYSNSYDPGFVYNVDELDMCVKSNRPDDFFDKLAFEGSPYE
                     GDDMIYPSAFLKKGEYSIIGGMRQRWEHDYDKNHNWLAHYSTLYFDHPEKDTIFTFGM
                     RYPYFEMGGEGYLHTNYFVNMCPKKDSNIVFLSLATTFDMEDKGREWITPELLEKTNW
                     PFQPSIIAIDMETKEAYIKHFFIDEVTGNHGGVFYFGDYLYLLVLYVTQTEPEYVFKR
                     ILYRFPESWLINEESKAKDTRMWIEDPEEIKEPQEKGLAELNPFLSVSAGGQHTCSLD
                     EKNSPICWGDNSQGQLGNSFEVVTNSYPYFINVKGYLNGKKVNVIKSGYSHVCAVTDD
                     YKAYCWGDNSSGALGNGGDVGSGEFEYYPSPVNTDGILKDKKITDVASGSIHTCVIDV
                     EGKGYCWGRNTLGSLGTETESQVVPEPVSVDMSGVLKDKKILEISSGSGHTCVVADDG
                     GVYCWGSNGRGQLGDGNGGNEGSLTELGDFSIVPVKSETAEGKYVKVSAGYSHTCILS
                     DKGKIY"
BASE COUNT         1060 a          504 c          767 g          993 t
ORIGIN      
        1 tatttcaata cacagcgaat taaaaataat ttgacatgaa acttagtcgc tgttagcata
       61 aaccgtgttg ttaatttaaa ataaatgaag aaagtgatga aaaccttgag tttaaagcag
      121 attctggcac acttattgct aattcattca tcactctttc ttacaaaaat ttcgttctgg
      181 ggtccctcct taaaaattag tcgggaaaac ggttcattta tttttgaaaa gatgtttttt
      241 taactaaact taaggagtta gagatgaaga aaatattggt ttttttgacc gcaatgttta
      301 tgtttgcagg tctttttgga gagggaaaaa tagttattga tgaaaaaact gctcaggaaa
      361 ttgaaaaact caaaagtgct ctcaaatctg ttggtgtgga atacgaactt ggcgctttta
      421 ttcctgaatc agatgaaaat ggagttgaag atgctttgac agttgacgag tttattgaaa
      481 gtcttgaatc tatgagtgta agatcaacag catacataag cgcatatgct ttaattccct
      541 tgtacggatg gtatcttgta aatggacatt gggttattga atggactgga aaggtttcgt
      601 cttcccctga tattgaaggt gatgattggg atggggttat gcacacaaga gagacttccg
      661 gcggaactgt ggttgaaaca aaaataacat ttaatccaac ttatgcatat ctatgggatg
      721 ctgtttatga aagcacacct agactttgtg cgcagctttt taacaatgta agggtttatg
      781 ttcccggaat tggatatatt ttggttcctc aagttttcgg aagtaagcat tgtggcactg
      841 taaattttaa ctggtagttt ttagcaaggg cagagaaaaa gctctgccct tcttttttag
      901 aggaggttcc tttgaaaacg gtatttattt taattgtcat ttttattgct tcatgcagtg
      961 gttcaccatc aaaaaatgat gaagatattc ttgttgacaa cgataccaac attaatgacg
     1021 aagaagcttc ggacgaagac attgagcata ctcctgcctg cataaatgaa tgggaaaaaa
     1081 ctgaaattgt tgaggatgtc agcgagttgt ttgattttga tgtcaaggtg gaaaccggaa
     1141 aatggaaatg ggaaaagaaa gataggcatg aagagctggt ttatggtgct gacccgagta
     1201 tgcctcttgg tgtaatgaat gacggcagtc tggtgttagg tgaaagtttt gaaactgaga
     1261 acggacattc aattgtttca attttaaacc ctgacggaac atttaagcgc tatgtccttg
     1321 aaattgaaaa aggaaatgaa ataaattatg gacaaggtga attaagtgca aatggattcg
     1381 agccgttcaa tttctggccg ggctattctt ttgatgaaga aaggcaggag attcttatcc
     1441 ctgtgactat aagatcaaaa ataaaaatcc ccggttccga agatgaatat ttcggtcgtc
     1501 cgtttctgat caaaatagat gctcaaggca atttgtcata tatcgcatgg aaaaataacg
     1561 gaaacagtgt ctgcaaccac ctcgttcaga ctgatgacaa gataataatg atgtgtctgc
     1621 actggagctc tgaccttgac atttatgttg caaccggaaa gataaatgct gaaataaatt
     1681 taatatataa aggaaatgtt tacagaaaac tcatgcagtc agattcctat gcttcaatgg
     1741 gtccgttttt aagctatgag aacggtaagg tatatgccca ctacagggaa gaatacagca
     1801 attcttatga ccctggattt gtttataatg ttgatgaact ggacatgtgt gtaaagtcaa
     1861 ataggcccga tgattttttt gataaattgg catttgaagg atctccttat gaaggtgatg
     1921 acatgatata tccttcagct ttcctgaaaa aaggggaata ttcgatcatc ggagggatga
     1981 gacaaagatg ggagcatgat tatgataaaa atcataattg gctggctcat tattcaacct
     2041 tatattttga tcatcccgaa aaagacacaa tttttacttt cggaatgaga tacccgtact
     2101 ttgaaatggg aggagaaggg tatcttcaca caaactattt cgtaaatatg tgcccgaaaa
     2161 aggacagcaa tatcgtgttc ttgtctttag caacaacatt tgacatggaa gacaaaggac
     2221 gagagtggat aacccctgaa ctgcttgaaa agacgaactg gccttttcag ccgtcaatta
     2281 tagcgataga tatggaaaca aaagaggcct atattaaaca ctttttcatc gatgaagtca
     2341 cgggcaatca tggaggagtg ttttattttg gagattattt atatttactg gtgctttatg
     2401 tgactcagac agagccggaa tatgttttca aaagaatttt ataccgtttc cctgaatcgt
     2461 ggcttataaa tgaggaatca aaggcaaaag atacccgtat gtggattgaa gatccggaag
     2521 aaattaagga accgcaggaa aagggattgg cggaactaaa tccattcctg tcggtttcag
     2581 caggcggaca gcatacatgt tctctggatg aaaaaaacag tccgatctgc tggggggata
     2641 attcacaagg acagcttgga aattcctttg aagttgtcac aaattcatat ccgtatttta
     2701 ttaatgtgaa gggttatctt aatggtaaaa aagtgaatgt catcaagtcg ggttattctc
     2761 atgtttgtgc tgtaacagat gattacaaag catattgctg gggagacaac agttcaggag
     2821 ctttagggaa tggaggtgat gtcggttcgg gagagtttga atattatcca tcaccagtta
     2881 atacagatgg tattttaaag gataaaaaaa tcacagatgt tgcttccgga tccattcata
     2941 cttgtgtaat tgatgttgaa ggcaagggct actgttgggg tagaaataca ctcgggagtc
     3001 ttggcactga aactgaatct caggtggttc ccgagccggt ttctgttgac atgagcggag
     3061 ttttgaaaga caaaaaaatt cttgaaatat cgagtggttc cggtcacact tgtgttgttg
     3121 cagatgatgg cggggtttac tgctggggat caaatggcag aggacagctt ggagatggaa
     3181 acggaggaaa tgaaggaagt ttaacggagc ttggagattt tagcattgtt cctgttaaat
     3241 ctgaaacagc ggaaggaaaa tatgtaaagg tttcagccgg ctattctcac acatgtattc
     3301 tgagtgacaa aggaaaaata tact
//
LOCUS       DAOOYB010000311         3317 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_34608_length_3317_cov_6.325567, whole genome shotgun sequence.
ACCESSION   DAOOYB010000311 DAOOYB010000000
VERSION     DAOOYB010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3317
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34608_length_3317_cov_6.325567"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="PL056_12630"
     CDS             <1..251
                     /locus_tag="PL056_12630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47909.1"
                     /translation="QRWELGLLINFDYYALAETYKAGQAGNKSKINDGNIAVGGGLLF
                     EYYTYSRHFSLGLSAEFNYVVDFDGMNIIVTPFMKYSF"
     gene            258..1073
                     /gene="murI"
                     /locus_tag="PL056_12635"
     CDS             258..1073
                     /gene="murI"
                     /locus_tag="PL056_12635"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HPM47910.1"
                     /translation="MNISVTQIISSSPIGIFDSGVGGLTVFKEVRRALPLEDIVYLGD
                     TARVPYGTKSVDVVKKYSKQNVEFLLSMGVKTIVVACNTASAASITYLNDIFRDVYII
                     GVIDPVVEHIKNNLSIKKVGVIGTATTIESNAYQNAIRKYIPDVKIFSKACPLFVPLV
                     EEGLFEDKITEIAIDMYLSAMKNENIDAIILGCTHYPMLKLAIERYFEKRVKVLDTAL
                     HTALTLKDELTNKNLLNLCGLGKERFYVTDSITTFKKTAEMFLGFNINDISHI"
     gene            1134..1874
                     /locus_tag="PL056_12640"
     CDS             1134..1874
                     /locus_tag="PL056_12640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HPM47911.1"
                     /translation="MKAVCLISLLFLLAFPNVSATENDSEDTINNTMQETKKDESTGI
                     TETSSMEDKKEADENTVENEQGYNLKLRTLEEKINSLKDRIFRSKQRLAILQETVLSG
                     TIAGARATITHKNEVGNAFKLVSAIYYLDDAPVFRKIDVPEELKKEEIVVFDGSVVPG
                     PHHISVFYVFAGKGYGLFSYMKGYTFKMKAGHSFHVEEGNLIEVTIAPKDKGASYKLD
                     NRLFTSFNISKKMFEEKESQREEKEQEN"
     gene            1891..>3317
                     /locus_tag="PL056_12645"
     CDS             1891..>3317
                     /locus_tag="PL056_12645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPM47912.1"
                     /translation="MKKTILFVYIVLVFLKVSVAQPNETSVSIDQKLDSIAKELEEIE
                     SECAGAKAKYLNPLMLNRDYKEPVLNKMKKADFYFDKEDYISSGSIYYSIVISQESKD
                     QIWEDAVYKLAESLFRNRNYISSARYFEMLVTDVKNSKYKIESLKRLISASYHLGDYS
                     VAKKYYTEFVEIGYDMSKDQDLIYFLAKSLFYDNQVDEATTVFSTVSVEGAYYPQSQY
                     FLGVIKLRKGLLQEALSYFEKVAEITQGSKYYKFERVFDLSVIAAARTAFELGDLIKS
                     VKYYVMLDKRSEHFAEAYYELCWTYIKKEEYVRAIDALRLIKYIAPDSIVAPKAEILE
                     GSLLIKLRRYGEAMVIFDQIVEKYSTIRDELNTIDNKSFLLNAKSGKLSKTLSPYSPV
                     VRSLLKDNKKFSSAMKLSDDISELEAEINRVNQLESKIGSIVDNKNAASLFPPLKEGS
                     SSALFLQGRIASIRNELIELRKTVI"
BASE COUNT         1163 a          503 c          661 g          990 t
ORIGIN      
        1 ctcaaagatg ggagctgggg cttctgatta attttgatta ctatgcactt gcagagacat
       61 ataaagctgg acaagcaggt aataaaagca agatcaacga cggcaatata gcagtcggcg
      121 gcggacttct tttcgaatat tatacatact ccagacactt ttcattaggt ttatctgctg
      181 aattcaatta tgttgtagat tttgacggaa tgaacatcat cgtcacgcct tttatgaaat
      241 attcgtttta actttccgtg aacatatctg ttacgcaaat aatttcatct tctccgatag
      301 gtattttcga tagcggtgtt ggaggcctta ctgtatttaa agaagttaga agggctctgc
      361 cattagaaga cattgtttat cttggcgaca cagcaagggt tccatatgga acaaaatctg
      421 tagatgttgt caaaaaatat tcgaaacaaa atgtcgagtt tcttctttca atgggcgtta
      481 agacaatagt tgttgcatgc aatacagcat ccgcggcatc gataacatat cttaatgata
      541 tttttcgaga tgtttatata atcggggtta tagatcctgt tgttgaacac ataaaaaaca
      601 atctttcaat taaaaaagta ggtgtaatcg gtaccgcaac taccatcgaa tccaatgctt
      661 accagaatgc tataagaaaa tatattcccg atgtaaaaat attctctaaa gcatgtccgc
      721 tatttgtgcc tttggtggag gagggtcttt ttgaagataa aataacagaa attgccatag
      781 atatgtatct gtctgctatg aagaacgaaa atattgatgc cataatttta ggatgtactc
      841 attatccgat gctgaaatta gcaatagaga ggtattttga gaaaagagtg aaggtgctgg
      901 atactgcttt acatacagcg cttaccctta aagatgagtt aactaacaag aatcttttaa
      961 atttatgcgg cctgggaaaa gaaaggtttt atgttactga ttcgatcacc acttttaaga
     1021 aaacagcaga aatgtttctg ggttttaata tcaatgatat ttcacacata taaaaattgc
     1081 tatttatatt taaattgtat atattcttcg cagaatttta tgggagttca aaaatgaaag
     1141 cagtttgttt aatttcactt ctttttttgc ttgcatttcc aaatgtttct gcaacagaaa
     1201 atgattcgga agataccata aataacacta tgcaggaaac aaaaaaagat gaaagcactg
     1261 gaatcacaga aacttcaagc atggaagata aaaaggaagc ggacgagaat actgtggaaa
     1321 atgaacaggg ttataatctt aaacttagaa cgctggaaga aaaaataaat tcactgaagg
     1381 atagaatttt caggtcaaaa cagagacttg caattttgca ggaaacagtc ttgagcggaa
     1441 caattgcagg agcaagagcg acgataactc ataaaaatga agtaggcaat gcattcaagc
     1501 ttgtttctgc aatatactat cttgatgatg cgccagtttt tagaaagata gatgtccctg
     1561 aagagctaaa aaaggaggag attgttgttt ttgacggttc tgtcgttccc ggtcctcacc
     1621 atatatctgt tttttatgtt tttgccggca aaggatacgg ccttttttca tatatgaaag
     1681 gatacacttt taaaatgaag gcaggacatt ctttccatgt tgaagaagga aatctgatag
     1741 aagttactat tgcaccaaaa gacaagggtg cttcgtataa gcttgataac agacttttta
     1801 catcattcaa tatatcaaaa aagatgtttg aagaaaaaga atctcagagg gaagaaaaag
     1861 agcaggaaaa ttgaagagcg aaagaatatt atgaaaaaga ccattttgtt tgtatacata
     1921 gttcttgttt ttctgaaggt tagtgtcgct caaccgaatg aaacatctgt aagtatagat
     1981 cagaaacttg attcaattgc aaaagagctt gaagaaatag aaagcgagtg tgccggggca
     2041 aaggcgaagt atcttaatcc tcttatgttg aacagggatt ataaagagcc ggtcttaaat
     2101 aaaatgaaaa aagctgattt ttattttgat aaagaagact acatttcttc aggcagcata
     2161 tactattcga tagtaatttc acaggaatct aaagaccaga tatgggaaga tgctgtttac
     2221 aaacttgcgg aatcgctctt tagaaataga aactatattt catctgcaag atattttgaa
     2281 atgctagtga ctgatgtcaa aaacagtaaa tacaaaatag aaagtttgaa aagacttatt
     2341 tcggcaagct atcatctggg agactattct gtagctaaaa aatattacac agaatttgtt
     2401 gagataggat atgatatgtc aaaggatcag gatttgatat acttccttgc gaaaagcctt
     2461 ttttatgaca atcaggttga tgaggcaact acagtttttt caacagtcag tgttgaaggg
     2521 gcatactatc ctcaaagtca gtatttctta ggagttataa aacttagaaa agggttgttg
     2581 caggaagctt tatcatattt tgagaaagtt gctgaaatta ctcaaggaag taaatattat
     2641 aaatttgaaa gggtttttga cttgtctgtt attgctgctg caaggacagc attcgaactg
     2701 ggagatctta taaaatctgt aaagtattat gtcatgcttg ataaaaggtc ggaacatttt
     2761 gcagaagcgt attatgaatt atgctggaca tacattaaga aagaagaata cgtcagagca
     2821 atagatgctc tcagacttat aaaatacatt gctccggact caatagttgc acctaaagcc
     2881 gaaatacttg aaggatctct tctgataaaa ttaagaagat atggcgaagc aatggtaatt
     2941 tttgaccaga ttgttgaaaa atatagcaca ataagagacg aactgaacac aattgacaac
     3001 aagtcatttc ttttaaatgc aaaatcaggg aagctttcca agacactttc gccctattca
     3061 cctgtagtaa gatcactcct taaagataac aagaaattta gtagcgcaat gaaacttagc
     3121 gacgacattt ctgagctgga agcggaaatc aacagggtta accagctcga aagcaagatt
     3181 ggctcaatag ttgataataa aaatgcggca tcattgtttc ctcctttaaa agaaggatcg
     3241 agttccgcat tgttccttca aggcaggata gcatctataa gaaatgaact tattgagttg
     3301 agaaaaactg ttatatg
//
LOCUS       DAOOYB010000312         3296 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_34936_length_3296_cov_5.847578, whole genome shotgun sequence.
ACCESSION   DAOOYB010000312 DAOOYB010000000
VERSION     DAOOYB010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34936_length_3296_cov_5.847578"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(125..571)
                     /locus_tag="PL056_12650"
     CDS             complement(125..571)
                     /locus_tag="PL056_12650"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPM47913.1"
                     /translation="MKEIKRASKDQAERLFEIIVKATGTGCTPFYPPEIMEIWHKGRS
                     NEGMAHVIETSEIYCLIDEGIIRGFLHIGEAEILGLFVHPDDHGKGYGTELFRFGVDK
                     IDSRPILIKATLNAVPFYSKLGCRKIRIESVRRHDHDIYVHRMELN"
     gene            complement(568..939)
                     /locus_tag="PL056_12655"
     CDS             complement(568..939)
                     /locus_tag="PL056_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015184381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPM47914.1"
                     /translation="MNPIYKTISEIKPFITTDGSLIREIFHPDRHNLPMSCSIAHASV
                     KVGESTKPHTLKKSSEIYVILSGQGIMHINDEKQLLNPGDAVFIPAGSVQYIENAGEE
                     DLNFLCVVDPYWKDEDDEVKK"
     gene            complement(952..1596)
                     /locus_tag="PL056_12660"
     CDS             complement(952..1596)
                     /locus_tag="PL056_12660"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPM47915.1"
                     /translation="MNLQQKYWNSVAETKNFPTPFQIDIFSDYVGKNKKILDVGCGYG
                     RVLNELYQAGFKELYGVDFSSGMIARGKRLYPELNLVVNEDGKLPFDNNSFDAVLLIA
                     VLTCMAEGSDQQRLMREIIRVLKPGGILYINDYLLNSDDRNIKRYEEFSEKYGTYGVF
                     ELPEGAVVRHHTRDYLGDLLKDFEEIVFEEVMYTTMNGNTSNGCYFMGKLNRIK"
     gene            complement(1688..3064)
                     /locus_tag="PL056_12665"
     CDS             complement(1688..3064)
                     /locus_tag="PL056_12665"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HPM47916.1"
                     /translation="MKNNKIKRVTENIMVETHLKNYFKRVTLEERMNQLNAPGVSITV
                     VNNGQIEWSNGFGLKNLSTGEKVRNDTIFLAGSVSKPVFALAVMKLVERGKIDLDRDV
                     NEYLKSWKIPENGAWQAEITMRQLLSHTAGLTVHGFGGYNQSSPVPTTKQILNGENPA
                     NSYPVVPYILPGTMFKYSGGGVTVAQFVVEELFDKPFPEIMDEILFKPLNLENSTYNQ
                     NLSQKQKEKAASGYYFNIRPVENGYHLYPEMAAAGLWTNGEDLAKIMIEVQSALKGES
                     LFFKKETVEEMLTVQKGTDDMGIGFFLEGESDSQRFFHAGWDEGFVTKFISGKQDGNG
                     VIVFINSNEGVGLLDEIVRSVANAYEWSEFNKDLHFAEEIDEKILDAYCGVYCDEKGL
                     EFKISKSTDKLLLELPGQKPLDVLSETFSFFDFKISFKTSETGEQIMTLVQDGNVITA
                     KKDENNLK"
     gene            complement(3079..>3296)
                     /locus_tag="PL056_12670"
     CDS             complement(3079..>3296)
                     /locus_tag="PL056_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47917.1"
                     /translation="TNACRINYEILGNSEPELHTHIFPRFDSEPDEKRRMPAWFYDWK
                     NAPQFSEKEHGELLRKIRTAIQKHLIE"
BASE COUNT          945 a          732 c          491 g         1128 t
ORIGIN      
        1 atgtcatcac acaatgctca ggagcatcaa tattttccca aaaactggaa gccttaagta
       61 aaggttcact gttgttatca aattctagga ttggatattt gttgttgttc attttttttc
      121 tccttcaatt caattccatc ctatgaacat aaatatcgtg atcgtgtcgc cttacagatt
      181 ctattctgat cttgcggcat ccaagttttg agtaaaatgg cactgcattt agagtcgctt
      241 tgatcagaat cggtcttgaa tctattttat caactccaaa acggaaaagt tctgtcccgt
      301 atccttttcc gtgatcatca ggatgaacaa aaagccctaa tatttcagct tcgccaatat
      361 gcaaaaaccc gcgaataatt ccttcgtcaa taagacaata aatctcagat gtttcaataa
      421 catgcgccat tccttcatta cttctgcctt tgtgccatat ttccatgatt tcaggcggat
      481 aaaaaggtgt acatccggtt cccgtagctt tgacaatgat ctcaaaaagt ctttcagctt
      541 gatcttttga tgctctttta atttctttca tttcttaacc tcatcatctt catctttcca
      601 atatggatca actacacaga gaaaattcaa gtcttcttca ccagcgtttt cgatatattg
      661 gactgatccg gcaggaataa acacagcatc ccccggattc agaagctgtt tttcatcatt
      721 aatatgcatg attccctgtc cactcaggat cacataaatt tccgatgatt ttttcagagt
      781 gtgcggtttt gtggattcac caactttcac agatgcatga gcaatactgc aactcatcgg
      841 cagattgtgt ctgtcaggat gaaatatttc acggatcagt gaaccgtcag ttgtaataaa
      901 aggtttgatc tctgaaattg ttttatatat cggattcacc agttttcctc ctcatttaat
      961 tctattcaat tttcccataa aataacatcc atttgatgtg tttccattca ttgttgtgta
     1021 cattacttcc tcaaaaacga tttcttcaaa atccttcagc aaatcaccaa gataatctct
     1081 ggtgtgatgc ctcacaacgg caccttccgg cagctcaaaa actccgtagg ttccatattt
     1141 ttcagaaaac tcttcatatc tttttatatt cctgtcatca ctgttgagta aatagtcatt
     1201 tatatagaga ataccaccgg gtttcaatac tcttataatt tcccgcatca acctttgctg
     1261 atcactgccc tctgccatac aggttaaaac tgcaatcagc agaactgcat caaaagaatt
     1321 attgtcaaaa ggaagtttcc cgtcctcatt aaccaccaga tttaactcag gataaagtct
     1381 ttttccccgt gcgatcattc ctgacgaaaa atccacaccg taaagctctt taaacccggc
     1441 ctgataaagc tcgtttagca ctctgccata tccgcatccc acatcgagta tctttttatt
     1501 ttttccaaca taatcagaaa aaatatcaat ctgaaacgga gttggaaaat tctttgtctc
     1561 tgcaacgctg ttccagtatt tttgctgtaa attcataatg tttatacccc cttcaaagtt
     1621 tcattcttac acgcatcaac aattgcctgc atatttgaac cgttgtgtga agctaagaat
     1681 gctaaattca ttttaaatta ttctcatctt tctttgcagt gataacattt ccatcttgaa
     1741 caagtgtcat tatttgctcc ccggtttcac tagttttgaa actgatttta aaatcaaaaa
     1801 agctgaaagt ttcagataaa acatccagag gtttctgtcc tggaagttca agaagaagct
     1861 tgtctgtact ctttgatatt ttaaattcca aacctttctc atcacaataa acgccacagt
     1921 atgcatcaag aattttttca tcaatttctt ctgcaaaatg aagatcttta ttaaattcgg
     1981 accattcata agcattggca acagaacgta ctatttcatc aagcaatcca acgccttcat
     2041 ttgaattaat aaaaacaatg actccattcc catcttgttt tccggagatg aatttagtga
     2101 caaacccctc atcccatcca gcatgaaaaa atctttgtga atcactttct ccttccagaa
     2161 aaaaaccgat tcccatatcg tctgtacctt tttgaacagt gagcatttct tcaactgtct
     2221 cttttttgaa gaataaagat tctcctttaa gtgcgctttg aacttcaatc atgatttttg
     2281 ccagatcttc gccgtttgtc cataatccag cagcagccat ctcaggatag agatgataac
     2341 cattttcaac aggtctgata ttgaaatagt agccggacgc tgccttttct ttttgttttt
     2401 gagaaagatt ttgattgtat gtgctgttct caagatttaa tggtttaaac aaaatctcat
     2461 ccataatttc tggaaaaggt ttgtcaaaaa gctcttctac aacaaattga gcaacagtta
     2521 cgccaccacc agaatattta aacattgttc ccggcagaat atatggaaca acaggatatg
     2581 aatttgccgg attttcacca tttagaatct gttttgtagt gggaactgga cttgattgat
     2641 tatatcctcc aaaaccatga actgttaacc ctgcagtatg acttaaaagt tgacgcatcg
     2701 ttatctctgc ctgccatgct ccgttctccg gaattttcca gcttttcaga tactcattca
     2761 catctctatc cagatcgatt ttgccccttt caaccagctt cataacagca agcgcaaaca
     2821 caggtttact tacagaacca gccaaaaata ttgtatcatt tcttaccttt tccccagttg
     2881 atagattttt aagcccaaat ccatttgacc attcaatctg accattattt acaacagtta
     2941 tgctgactcc aggagcattc aactgattca ttctctcttc cagtgttaca cgcttgaaat
     3001 aatttttcag gtgtgtctcc accattatat tttctgtaac tcttttgatt ttattgtttt
     3061 tcatttatta actccttttc attcaattaa atgcttttgt atcgcagttc ttatcttcct
     3121 cagtaattct ccatgttctt tttcagaaaa ctgcggtgca tttttccaat catagaacca
     3181 tgcaggcatg cgtctttttt catcgggttc agaatcgaac ctcggaaata tgtgggtatg
     3241 aagttcaggt tcggaatttc ccagtatttc ataatttatc cggcaggcat ttgtga
//
LOCUS       DAOOYB010000313         3281 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_35147_length_3281_cov_11.563856, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000313 DAOOYB010000000
VERSION     DAOOYB010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35147_length_3281_cov_11.563856"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..192)
                     /locus_tag="PL056_12675"
     tRNA            complement(98..192)
                     /locus_tag="PL056_12675"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(158..160),aa:Sec,seq:tca)
     gene            complement(252..866)
                     /locus_tag="PL056_12680"
     CDS             complement(252..866)
                     /locus_tag="PL056_12680"
                     /inference="COORDINATES: protein motif:HMM:NF025041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA N-terminal domain-containing protein"
                     /protein_id="HPM47918.1"
                     /translation="MSLKIDKITAIDVYAEFGKSRILVGRLNKIKDKFQFTYDRKYLK
                     SEKKLSLGPEFPMTKIRFFSDSLFNSFKDRIPSKENPAYSEYCEKFGVSSDENNELIL
                     LATIGSRGPSSFVFEPVFDSEFTAVDLAAFRKKLKLSIRDFAILFDFSYATLNKIENG
                     KMSGRDILKRIEIYALFKETAFFEIERNKAKVHSKVYESVKTLL"
     gene            complement(850..>1674)
                     /locus_tag="PL056_12685"
     CDS             complement(850..>1674)
                     /locus_tag="PL056_12685"
                     /inference="COORDINATES: protein motif:HMM:NF019421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HPM47919.1"
                     /translation="LSEAIEFAGVARKETDSDKERNEPFSQFNSSFFQGKFRKYSASL
                     NGVDYILKVEEKEYPELPATEFLCNEIARFFKIEVPEFYLIDFHGRNAFVSKNFINKN
                     KIMALHHIYHYFQKDELFDVETLIKIIEKQTGQVSEVERFVKLCLFDALIGNHDRHGR
                     NLAFIEEAKGHTVLSPFYDNPTYIGVEEEMLLGAQHDPKGKIATRLTNEPTMKDYATE
                     FIETGHRIPVEEFMNGIDLDKLGIIVSESFISPKRKTALMKIIYERTMELRNVLKN"
     assembly_gap    1675..1774
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1775..3132)
                     /gene="hydG"
                     /locus_tag="PL056_12690"
     CDS             complement(<1775..3132)
                     /gene="hydG"
                     /locus_tag="PL056_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HPM47920.1"
                     /translation="MNTSFIDHGRIYDYLKNTSEPDSAELNEILDRARELKGISYAAA
                     ERLLMVEKPEHLEKIFETASFIKNEIYGCRLVLFAPLYVSNMCNNECLYCAFKRSNRE
                     ITRRTLTQEEIRHEVEALVSQGHKRVLLVSGEGLGRSALDYTLQAIDTIYSTKVGKGE
                     IRRINVNIAALEVDDFRKLKDAKIGTYQLFQETYNINTYKKMHVAGPKSDYAYHLGAM
                     DRAMEAGIQDVGVGILFGLEDYRFEVMALLQHIAHLEERFGFGVHTISVPRLEPATGS
                     DVSVHPPYDIDDTTFKKIIAILRITVPYTGIILSTRETAETRRNAIEMGISQISAGSR
                     TNPGGYSDENSAEQFSLGDHRSLEEVILDIAKMGHVPSFCTGCYRLGRVGQDFMDLAK
                     PGLIKHYCLPNAMTSFYEYLVNYAKPETKSIGRELIDRMMNDIPNSDLRERTKKALTD
                     IEA"
BASE COUNT          899 a          682 c          584 g         1016 t
ORIGIN      
        1 tcaaaacatc aatcgagggc aggaatcgaa cctgcttggt aaaaccggct gagaaacaca
       61 gcccggagca ccagctccac ctgaaaaagc aaaaagttgg cagagagggc aggaatcgaa
      121 cctgccacgg acggttttat ccgcccgcta ctggttttga agaccagttg agccaccaga
      181 gtcgtcctct ccacatgtga ttaaaatata aagggctgtt agtgaagtca aattttttca
      241 agagttgagt tctacagtaa ggttttaact gactcataaa cttttgagtg cactttcgct
      301 ttattccttt caatttcaaa aaaagccgtt tctttgaata aggcataaat ttcaattctt
      361 ttcaatatgt cccgtcccga catttttcca ttttctattt tgttcaaagt agcatatgaa
      421 aaatcaaaaa gtattgcgaa atcacggatt gaaagtttca gtttttttct aaatgcagca
      481 aggtcaacag ccgtaaattc cgaatcaaag accggttcaa atacaaaact tgacggacct
      541 cggcttccta ttgtcgcaag aaggataagt tcgttatttt catcagaaga aactccgaat
      601 ttttcacaat attcgctgta tgcagggttt tcttttgaag gaatcctgtc cttaaagctg
      661 ttaaaaagtg aatccgaaaa aaaccgtatt tttgtcatgg gaaattcagg tcctaatgaa
      721 agtttttttt cacttttaag atattttctg tcatatgtaa actgaaattt gtcttttatt
      781 ttgtttaatc gtccaaccag tattctgctt ttcccaaatt cagcataaac atcaatagct
      841 gttatcttat caatttttaa ggacatttct cagctccatt gttctttcat aaataatttt
      901 catcaacgcc gtcttccttt ttggggaaat aaacgattct gaaacaatta tccccagctt
      961 atccagatcg ataccattca taaattcttc aaccggaatt ctgtgaccgg tttcaataaa
     1021 ttcagtggca taatccttca tcgtcggctc atttgtaagt ctcgtcgcaa tttttccttt
     1081 tggatcatgc tgtgcaccta acaacatctc ttcttcaact ccaatgtaag tcggattgtc
     1141 ataaaatggt gaaagcacag tatgaccttt tgcttcttca ataaaagcca gatttctgcc
     1201 atgtctgtca tgatttccta ttaatgcgtc aaacaggcaa agtttaacaa atctttcaac
     1261 ttctgaaacc tgccctgtct gtttttcaat aattttgatc aaagtctcta catcaaataa
     1321 ttcatctttc tggaaatagt gataaatgtg gtgaagtgcc atgatcttat ttttgttgat
     1381 gaaattctta ctcacaaaag catttctgcc gtggaaatca atcaggtaga actcaggcac
     1441 ttctatttta aaaaatcttg caatttcgtt acagagaaat tctgtcgcag gaagttccgg
     1501 atattctttc tcttcaactt tcagaatgta gtcaacacca tttaaagagg cagaatattt
     1561 tcgaaacttg ccttgaaaaa aacttgaatt gaattgtgaa aacggctcat ttctttcttt
     1621 gtcagaatct gtttcttttc gtgccactcc ggcaaattct attgcttcag aaagnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngcttcg atatcggtga gagctttttt
     1801 tgtcctttct cttaaatcgc tgttgggaat gtcgttcatc atcctgtcga taagctcgcg
     1861 tcctattgat tttgtttccg gtttcgcata gttgacaaga tactcgtaaa aactggtcat
     1921 cgcattcggg agacagtaat gttttataag ccccggtttt gcaaggtcca tgaaatcttg
     1981 acccactctc cccagccggt aacagccggt gcagaaactt ggaacatgtc ccatttttgc
     2041 aatatcgaga atcacttctt caagcgagcg gtggtcgcca agcgagaact gttctgcaga
     2101 attttcgtct gaataaccgc ccggatttgt ccttgagccg gcacttatct gcgaaatacc
     2161 catttcgatt gcatttctcc ttgtttccgc agtctctctt gtggaaagaa ttatcccggt
     2221 atatggaacg gttatcctca atatcgcaat tattttcttg aatgtagtgt catcaatgtc
     2281 atacggcggg tggactgaaa catctgagcc ggttgccggt tcaagacggg gaacggaaat
     2341 cgtgtggact ccgaaaccga acctttcttc aagatgggca atgtgctgaa ggagcgccat
     2401 aacttcaaaa cggtaatctt caagcccgaa aaggattccg acaccgacat cctgaatacc
     2461 agcttccatc gctctgtcca tcgctcccag atgatatgca taatcagatt tagggcctgc
     2521 gacatgcatc tttttatagg tgtttatgtt gtaagtttcc tgaaaaagct gatatgtacc
     2581 gatctttgca tctttaagtt ttctgaaatc atcaacttca agagcggcaa tgttgacatt
     2641 tatccttctg atctcacctt tgccgacttt tgttgaatag atcgtgtcaa ttgcctgaag
     2701 tgtgtaatca agagcacttc tgccaagccc ttcacccgaa acgagcagaa cccttttgtg
     2761 accctgactc acgagcgctt caacttcgtg ccttatctct tcctgagtaa gtgtccttct
     2821 tgttatttct ctgttgcttc tcttgaaagc acagtaaaga cactcgttgt tacacatgtt
     2881 actgacataa agaggcgcaa aaaggacaag ccggcagccg tatatctcgt tcttaatgaa
     2941 gcttgccgtc tcaaatatct tttcaagatg ttcgggtttt tcaaccatca gaagccgctc
     3001 cgcagcggca tagcttatcc ctttgagttc ccttgcacgg tcaaggatct cattcagttc
     3061 ggctgaatcc ggttcactgg tgtttttaag ataatcataa attcttccgt gatcaataaa
     3121 agaagtattc ataacagatc tccgcaaaaa gaaaattgat aaaaaggaac ctctaattaa
     3181 aagaaagata atactttaaa ttctattttg caagatgtga ttttgatttt tgaagttccc
     3241 ccaaaaaaaa ttcagcagat ttctaactga agaggaggag c
//
LOCUS       DAOOYB010000314         3227 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_35981_length_3227_cov_4.412673, whole genome shotgun sequence.
ACCESSION   DAOOYB010000314 DAOOYB010000000
VERSION     DAOOYB010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35981_length_3227_cov_4.412673"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..716)
                     /locus_tag="PL056_12695"
     CDS             complement(<1..716)
                     /locus_tag="PL056_12695"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HPM47921.1"
                     /translation="MSRGEVMGEEIKRLIDSGENERVEFKESWRDEFLKHICAFANTE
                     GGVLLVGVKDNGIIAGISNSSRLLEDIPNKVIQALGIIVTVGVHNIELKNIIEIKVPV
                     SSVPISFRGRFYVRSGSTVQELHGHELRDFILRKDNVTWDSIAVSEASVEEIDINVVR
                     YFATKAVTEKRLYSEALSEDISTTLKKLDLINEAGKLTKAAILLFGKRPGKYIRTAIV
                     KIGRFGATPADLISHDIIEG"
     gene            complement(735..1181)
                     /locus_tag="PL056_12700"
     CDS             complement(735..1181)
                     /locus_tag="PL056_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009544220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47922.1"
                     /translation="MIFTVSSNKNLIAIIIPANYRSDGVEFFTPDSFSQQLAFMSHKK
                     GKVIDAHVHNHNPREVVYTQEVLVMRKGKLRVDFYDDERNYLESRVLEAGDVILLASG
                     GHGFEVIEDVEMIEIKQGPFCGEADKTRFVGVTRGEVKSDERGSDE"
     gene            complement(1178..2038)
                     /locus_tag="PL056_12705"
     CDS             complement(1178..2038)
                     /locus_tag="PL056_12705"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPM47923.1"
                     /translation="MNPEIIIILPVHNRRETTRKFLECLIGQTFQHYHLILIDDGCID
                     GTPEMSESLIKNITILKGNGNLWWAGSLHLAYKHLKETHRFQDDDIVLIINDDVFIKP
                     DFLSIAAELFKQNNGRKILSQAIAIDKSTGKELPGVFYDNKAISFEPTKHIDRVNCMS
                     TRGLLLRKSDFTDSGGFHPCILRHYLSDYEFTNRLISKRNFIPVMEERFVLEFFPETS
                     GFENPTNKDKICNYLSDLFSKKNKSNPIHWIVFIFLTTQFPYNFKNVLKIIYLNVRQT
                     AVVLKRSIDK"
     gene            complement(2035..>3227)
                     /locus_tag="PL056_12710"
     CDS             complement(2035..>3227)
                     /locus_tag="PL056_12710"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPM47924.1"
                     /translation="NEIGEISDLIKKYNAGFVADMASDDPFKMLTNFESSENFTESCH
                     ELFSENLSIGKFIGVVIEKINKETTHFTGFDHTNCPVCNSSKIKSKFNKYDDRYACPG
                     IFKIGKCSNCGHQFVFNAKGLNIGKLYTEFYPRKNIQNGYVEPVFEKKSVFDEWLNGD
                     KRSAFRHVPEDVKALDVGCGDCRSLFYHKSRGCDATGIEADENVAFLKEKFGDRLIIG
                     EFKRDLFKEGAFDYVTMDQVVEHFENPLKKLMEVKEIVRNGGKIVLSTPNSSGWGAKL
                     FGKYWINWHTPYHLNLFSKKSIRIALETAGFKNIQIKTVTSSEWLNYQWSHILFYPAN
                     GEPSYFWSDEEKKKTIKVKKGRRKLNRYIHNVLHKIKINHIITRIFDAIGVGDNMLVI
                     AEKK"
BASE COUNT          934 a          712 c          490 g         1091 t
ORIGIN      
        1 tttccctcaa ttatatcgtg acttataaga tctgccggtg ttgccccgaa tcttccgatc
       61 tttactattg cggttctgat gtattttccc ggtctttttc caaaaagtaa tatcgcggct
      121 tttgtaagct ttccggcttc gtttataaga tcaagctttt ttaaagtagt tgatatgtct
      181 tcgctcaatg cttctgaata aagccgtttt tcagtcaccg cttttgtggc aaaataacgg
      241 acaacattaa tatcaatctc ctcgaccgaa gcttcagaaa cagcaatgga atcccaagtg
      301 acattgtctt ttcttaaaat aaaatctcta agttcatgac catgcaactc ctgaacagta
      361 ctgccgcttc tgacataaaa cctgccacga aaagaaattg gaacggaact gacgggaacc
      421 tttatttcaa tgatattttt caattcaata ttgtgcacac cgactgtcac aataatacct
      481 aacgcctgaa tgactttatt tggaatatcc tctaaaagtc tgctgctgtt acttattcct
      541 gcaataattc cattatcttt tacaccgaca agaagaactc ctccttcagt atttgcaaat
      601 gcacaaatat gtttcagaaa ttcatctctc catgactcct tgaactctac ccgttcattt
      661 tcaccagaat caatcagtct cttaatctct tcacccatca cttcccctct gctcactcgt
      721 cacttcccct ctcgtcactc gtcacttccc ctctcgtcac tcttcacttc ccctctcgtc
      781 actccgacaa aacgggtttt gtcggcttcc ccacaaaacg gaccctgctt tatttcaatc
      841 atttcaacat cttcgatcac ctcaaaaccg tgacccccgc ttgcaagaag aataacatcc
      901 cctgcctcaa gaacacggct ttcaagataa tttctctcat catcataaaa atcgactctc
      961 agctttccct tcctcatcac aagcacttcc tgagtataaa ccacttctct tggattgtga
     1021 ttgtgaacat gtgcatcaat aacttttccc tttttgtgac tcataaaagc caattgctgc
     1081 gaaaatgaat ccggagtgaa aaattcaaca ccatcggatc tgtaatttgc ggggataatt
     1141 atggcaataa ggtttttgtt gcttgacaca gtaaaaatca tttatctatg ctccttttca
     1201 aaacgacagc agtttgtctt acatttaagt atatgatttt aagaacattt ttgaaattat
     1261 atggaaactg tgtcgtgaga aatataaaaa cgatccagtg aatcgggttg gatttattct
     1321 tttttgaaaa cagatctgac aaatagttac agattttatc tttatttgtt ggattctcaa
     1381 acccgctagt ttctggaaag aactccagga caaatctttc ctccatgaca ggaataaaat
     1441 tccttttgct gatcaacctg tttgtgaatt cgtaatccga caaatagtgg cgcagaatgc
     1501 acggatggaa accaccgcta tcggtaaaat cactttttct caacaacaat cctcttgttg
     1561 acatacagtt gactctgtca atatgcttag tgggttcaaa tgatatggct ttattatcat
     1621 aaaaaacccc tggcagttct ttaccggtac ttttatcaat tgcaattgcc tgagacaata
     1681 tttttcttcc attgttttgc ttaaataatt cagcagcgat acttaagaaa tccggtttaa
     1741 tgaaaacatc atcgttaatt atcagaacga tatcatcatc ctgaaaccgg tgcgtttctt
     1801 ttaaatgttt ataggcaaga tgtaaagaac cagcccacca taaatttcca tttcctttga
     1861 gaattgtgat atttttgatg agcgattccg acatttcagg agtaccgtct atacaaccat
     1921 catcaataag aatgagatga tagtgctgga atgtctgacc gatcaaacac tcaaggaatt
     1981 tccgagtcgt ttcccttcgg ttgtgtaccg gaagaattat tataatttca ggattcattt
     2041 cttttcagca atcacaagca tattgtcacc aactcctatg gcatcaaaaa tacgcgtaat
     2101 tatatggttt atctttattt tgtgcaatac attatgaata tacctgttga gctttcttct
     2161 tccttttttc acttttatcg tttttttctt ttcttcatca ctccagaaat aggacggctc
     2221 tccatttgca gggtaaaaca aaatgtgtga ccactgatag ttcaaccatt cagagcttgt
     2281 aactgttttt atttgaatat tcttaaatcc tgcggtttcc agagctattc ttattgattt
     2341 ttttgaaaag aggttgaggt gatatggagt gtgccagttt atccagtatt tcccaaacag
     2401 ttttgctccc catccggaag aattgggcgt agaaagaact attttgccgc cattcctcac
     2461 tatttcttta acttccataa gttttttcaa aggattttca aaatgttcaa caacctgatc
     2521 cattgtaaca taatcgaagg caccttcttt aaacaggtct cgtttgaatt caccgattat
     2581 aagtctatca ccaaattttt cctttaaaaa cgcaacattt tcatctgctt caatgccagt
     2641 cgcatcgcat cctctacttt tatgataaaa caaagacctg caatcaccac aaccaacatc
     2701 caaagcttta acatcttcag gaacatgtct aaaagctgat cttttgtctc cgttaagcca
     2761 ctcatcgaaa acgctttttt tctcaaacac tggttcaaca tagccattct gaatgttttt
     2821 tctgggatag aactccgtat aaagttttcc gatgttgagt ccctttgcat taaaaacaaa
     2881 ttggtgtccg caattcgaac atttccctat cttgaaaatt ccaggacagg cataacggtc
     2941 atcgtatttg ttgaatttgc tttttatttt tgatgagtta caaactggac aatttgtatg
     3001 atcaaatccg gtgaaatgag tggtttcttt gtttattttt tcaattacca ctccaatgaa
     3061 ttttccaatt gaaaggtttt cactgaaaag ttcgtgacat gattcagtaa aattctcaga
     3121 tgattcaaaa ttcgtcaaca ttttaaaagg gtcatctgat gccatatcag caacaaaacc
     3181 ggcattatat ttctttatca agtcagatat ttctccaatt tcgttaa
//
LOCUS       DAOOYB010000315         3217 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_36137_length_3217_cov_11.724541, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000315 DAOOYB010000000
VERSION     DAOOYB010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3217
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36137_length_3217_cov_11.724541"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="PL056_12715"
     CDS             <1..613
                     /locus_tag="PL056_12715"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HPM47925.1"
                     /translation="VTIRIFQGEREIAAQNKLLGEFDLKDIPPAPRGLPKIEVSFDID
                     ANGILNVSAKDLGTNKQNTIVIKAGSGLTDEEIERMVADAERNKEEDEKRKKVVEAKN
                     NADQMVYSTEKALKEYGEKIDPQAKAEIEEKLSALKAASEHEDAETIEKAISELNESA
                     HKLAEAMYKNAGGAPDQQGDTGQSDGANKKDDGVVDADFEEKK"
     gene            620..1741
                     /gene="dnaJ"
                     /locus_tag="PL056_12720"
     CDS             620..1741
                     /gene="dnaJ"
                     /locus_tag="PL056_12720"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HPM47926.1"
                     /translation="MADFYELLGVSRNASNEEIKKAYKKQALKHHPDRNKGDKGAEEK
                     FKKINEAYEVLSDPEKRQMYDQFGEDGLKANPFQGQNGGYRDFSDVFDNLGDMFGDIF
                     GGRGSRRGRKVPTQGSDLLVHLTISFEESFTGTKKDISFTRSSTCQVCLGTGAEHGSS
                     RKTCPTCNGAGQVRVSHGIFSLAQTCPTCRGEGTIIEKPCSHCRGTGFVKEDKKISLN
                     VPPGISSGMKMRVAGEANSGTNGGPRGDVYVEIRVKDHSLFEREGDDIYVEIPISYSQ
                     AVLGDRIEVPTMTGPVEMKIPAGTQPGTKMRLKDKGFPSLQRGGGIGSLYVILTLEVP
                     KNISEKHREHILKLKEYEADHKERPSFKDYLDKAKNLFK"
     gene            1741..2736
                     /locus_tag="PL056_12725"
     CDS             1741..2736
                     /locus_tag="PL056_12725"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPM47927.1"
                     /translation="MGKSYDVIIIGAGPGGLECARGFADKEMSVLVIEKNDIVGPKTC
                     GGALTNLTEKFNIPDKITETFQAIKLEINRDSIDLDLKKPIRTVSRSDLGQYLLNILR
                     SSKNIEIITGTEVESVCEGKVFTGKEEFSYKYLVGADGSDSVVRKFLGLKSEFRTGLL
                     YRIESVRALFRWSVNPQKLGSGYMWMFPNKKGGNAGIYFDQDRINNSNALKILDEFIV
                     SSGLKINNSKVQHGKIQHKYSGVSFGNIFLIGDAAGLPLKNTGEGIPGALISGKEIAK
                     KILNRNYDMPELRKYIKLKKRQEKLLKIFEAVPFLQSFLFKLYAVYKRLQIFCRV"
     gene            2901..>3217
                     /locus_tag="PL056_12730"
     CDS             2901..>3217
                     /locus_tag="PL056_12730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47928.1"
                     /translation="MSKYFILLGFICVFWFASCSVKDDVKVDDLNQNDENEQKNDDLE
                     TNDDNSVKDDSGQVDDVVKTDDSATPDNNIVPDDSVMIDEDQTTDEDLTDNEESDLND
                     ESG"
BASE COUNT         1071 a          543 c          776 g          827 t
ORIGIN      
        1 tgttacgata aggatcttcc agggagaaag agagatcgct gcacagaata aacttcttgg
       61 cgaatttgat ctgaaggata ttccgccggc tccgagaggt ctgccgaaga tcgaggtgag
      121 ttttgacata gatgcaaacg gaattctgaa tgtcagcgca aaagacctcg gcacaaacaa
      181 gcagaacaca atagtcatca aagcaggcag cggacttact gatgaagaga ttgaaagaat
      241 ggttgcagat gctgagagaa acaaggaaga agatgaaaag agaaagaagg ttgttgaggc
      301 aaagaacaat gcagatcaga tggtatattc aactgaaaaa gcattgaagg aatatggtga
      361 aaaaatagat cctcaggcaa aagctgaaat agaagaaaaa ctttctgcat tgaaagccgc
      421 ttcagaacat gaagatgctg aaacaataga aaaggcgatc agtgaactca atgagagtgc
      481 acacaagctt gctgaagcaa tgtataaaaa tgcgggcggt gctcctgacc agcagggtga
      541 cacaggacag agtgacggtg cgaacaaaaa agatgatggt gttgttgatg ccgattttga
      601 ggagaaaaaa taaactcaca tggctgattt ctatgaactg ctcggcgttt caaggaacgc
      661 atcaaacgaa gaaataaaga aagcatataa aaagcaggcg cttaaacatc atcccgatcg
      721 caacaagggt gacaagggtg ctgaagagaa attcaaaaag atcaacgaag cttacgaagt
      781 tctgagcgat ccggaaaaaa ggcagatgta cgatcagttc ggagaggacg ggttgaaggc
      841 caatcctttt cagggacaga acggcggata cagggatttt tccgatgtgt tcgataatct
      901 gggcgatatg ttcggagata ttttcggcgg tcgcggatca agaagaggaa ggaaggtgcc
      961 cacacagggt tccgaccttc ttgtccatct gacgataagc tttgaagagt cgttcacagg
     1021 gactaaaaaa gatataagtt ttacgagatc atcgacctgt caggtctgtc ttggaacagg
     1081 ggcggaacac ggaagttcaa gaaagacatg ccccacatgc aacggtgccg gccaggtaag
     1141 ggtttcacac ggtatttttt ctcttgctca aacctgtccg acatgtcgtg gtgagggaac
     1201 gataattgaa aaaccgtgtt ctcactgtcg aggcactggt ttcgtaaaag aggataagaa
     1261 gatctcactc aatgttcctc ccggtataag ttcaggaatg aaaatgagag ttgccggtga
     1321 agcgaattcg ggaaccaacg gcggtcccag aggcgatgtg tatgttgaga taagggtaaa
     1381 agatcattcg ctttttgaaa gagaagggga tgatatttat gttgagatcc cgatatctta
     1441 ttctcaggcg gttctcggag acaggatcga agttccgact atgaccggtc ctgttgaaat
     1501 gaagatacct gccgggactc agcccggaac aaaaatgcgg cttaaggaca agggattccc
     1561 ttcactccaa agaggcggcg gcataggttc tctttatgtc attcttacac tcgaagtgcc
     1621 taaaaatatt tcagaaaaac accgtgaaca catcttgaaa cttaaagaat atgaagcaga
     1681 tcacaaagaa agaccatcat ttaaagacta tctcgataaa gcaaaaaatc tgttcaaata
     1741 atgggaaaaa gttacgatgt aataattatc ggagcaggtc ccggcggtct tgaatgtgca
     1801 agaggttttg cagacaaaga aatgtctgtg cttgtaatag aaaaaaatga tattgtcggt
     1861 cccaaaacat gtggcggcgc gcttacgaat cttactgaaa aattcaatat tcctgataaa
     1921 ataactgaaa catttcaagc aattaaactt gaaataaaca gagattccat cgatcttgat
     1981 ctgaaaaaac cgataagaac tgtatcaagg tctgatctgg ggcaatatct gctgaatata
     2041 ttaagatcgt ctaaaaacat tgagatcatt acagggacag aagttgagtc agtttgtgaa
     2101 gggaaagttt ttaccggtaa agaggagttt tcttacaaat atcttgtcgg tgcagatgga
     2161 tctgattctg ttgtacgaaa gtttctcggg cttaaaagtg aattcaggac aggtttgctt
     2221 tatcggatag aaagtgtcag agctcttttc aggtggtctg tcaaccctca aaaactaggt
     2281 tccggatata tgtggatgtt tccgaataaa aaggggggca atgccggaat ttattttgat
     2341 caggaccgga taaataacag taatgccctg aaaattctgg acgaatttat cgtttcatcg
     2401 ggactgaaaa ttaataacag caaagttcag catgggaaaa ttcagcacaa atattcaggt
     2461 gtttctttcg gtaatatttt tcttattgga gatgccgcag gacttccgtt gaagaatacc
     2521 ggggagggaa ttcccggagc attgataagc ggtaaagaga tcgcaaagaa aatattgaac
     2581 agaaactatg atatgccgga actcagaaag tatatcaaat tgaaaaaacg gcaggaaaaa
     2641 ctactgaaaa tttttgaagc agttcctttt ctgcaatcat ttctctttaa actctatgct
     2701 gtttataaaa gattgcagat cttttgcaga gtgtgaaggg ataaaagatt ctttctttag
     2761 tcaaatcggt tctgatttaa aaatatggac atgtcttttc gtctattccg gttcattttt
     2821 ctcaagacgg gctatatctc cttaattttc attattatcc aaactgtttg gtgcggttaa
     2881 catttttttt gaggtttaag atgtctaagt attttattct gctgggtttt atctgtgttt
     2941 tttggtttgc gtcctgctca gttaaagatg atgttaaggt tgatgatctc aatcagaatg
     3001 acgaaaatga gcagaaaaac gatgatttag aaacaaatga tgataattct gtaaaagacg
     3061 attccgggca ggttgatgat gttgtcaaaa cagatgacag cgcaactcct gacaataata
     3121 ttgtgcctga cgattctgtc atgatagatg aagatcagac aacggatgaa gacttaactg
     3181 acaatgaaga gagtgattta aatgacgaaa gcggaaa
//
LOCUS       DAOOYB010000316         3211 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_36238_length_3211_cov_7.576679, whole genome shotgun sequence.
ACCESSION   DAOOYB010000316 DAOOYB010000000
VERSION     DAOOYB010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36238_length_3211_cov_7.576679"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="PL056_12735"
     CDS             complement(<1..358)
                     /locus_tag="PL056_12735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47929.1"
                     /translation="MKNLSFKFVTLSLLTFLLISFTAYILNFQFIHITVFSLIFTLFY
                     SEKKIRHRITYIVIALLIYSQFATPMPVTFILLVSIQLYFLTVSFFESTAFDFSLTAL
                     INSILSVIVININRLIY"
     gene            complement(339..1166)
                     /gene="mreC"
                     /locus_tag="PL056_12740"
     CDS             complement(339..1166)
                     /gene="mreC"
                     /locus_tag="PL056_12740"
                     /inference="COORDINATES: protein motif:HMM:NF016011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HPM47930.1"
                     /translation="MNRKKIFLYILAVSIVLAFAIPNFILEYTPRSVAADSMKSFFQH
                     QIETVRNTVQIIIGLKNVGKKNIELEREISELKVRLIMNEAIKKENEELSKLLKLKDS
                     YGKYTIIPARILNFSDINPNRIVVSFPQEYSKLMAEKATVVSSMGLVGLVSSFHTSRA
                     EVELITSKQFTIPAVLENREECTAILKGNGQSLSILFLDKVCNIPSADGKKLLSANLS
                     ENYSIPYLPIGIISSLKEDEGNILFTRGEAIPLFKKGKLNHIFIIVGTNTKDEKPLF"
     gene            complement(1168..2208)
                     /locus_tag="PL056_12745"
     CDS             complement(1168..2208)
                     /locus_tag="PL056_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HPM47931.1"
                     /translation="MFKIDNLLSIFTSDIAIDLGTANTLIYVKGIGIVANEPSVVAVQ
                     EKDSRGAKSVRAVGREAKEMIGKAPVNISVIRPLKDGVIADFEVAHQMLRYFIRHAYR
                     NGSFIKPRIAIGVPYGITEVEKRTVKEAALGAGAREVFLIEQPMAAAVGANMPVTEPT
                     GNMIVDIGGGTTEVAVISLAGIVYSKSVKSAGDKMDESIIDYIKKKYNLAIGERMAEQ
                     IKWTIGNAYPDETKEFMEVKGLDLIAGVPKTIEINSDEIREALEEVVKAIVSAVKTVL
                     ENTPPELAADIVDKGIVLAGGGALLKNLDILLREETNLPVIIADDPLKSVVMGTGKIL
                     ENISLLKTISLY"
     gene            2376..>3211
                     /locus_tag="PL056_12750"
     CDS             2376..>3211
                     /locus_tag="PL056_12750"
                     /inference="COORDINATES: protein motif:HMM:NF016827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47932.1"
                     /translation="MAPSIKLQQSLVQKQELKMTVQMRQSIEMLLKPAFELRDMILAE
                     AEENPFLEIEEWGDGNDDDVVHGPVEPVEESLQDNGDPSPLKIGDENADMSDILAEFD
                     WDQVRESSSNSFGETIVRKKNDSSDDFSFEDFVPASESFNQFLDFQICSLQVTSVLKD
                     IMIYMTYNLNEKGFLEDSDASVAEMTGSEVTDVEKARQLLKLMDPKGCGCHDLVDYLR
                     FMFTEVMKDDVPDRFSLSVQKLFHDEDMLELLIKKNFDSLCEKLSLDKEDLTELLQFF
                     RKG"
BASE COUNT          883 a          626 c          703 g          999 t
ORIGIN      
        1 gatatatgag ccggtttatg ttgatgacaa taaccgaaag aattgagttt atcagtgctg
       61 taagtgaaaa atcaaaagct gtggattcaa agaatgaaac tgtaagaaag taaagctgta
      121 ttgagaccag cagaatgaat gtaaccggca tgggtgttgc aaactgtgaa tatatcagga
      181 gtgcgataac gatatatgtg atcctgtgtc ttattttctt ttcggaatag aacagtgtga
      241 agatcagcga gaaaaccgtt atgtgaatga actgaaaatt taaaatatag gcggtgaaag
      301 atattaaaag aaaagtaagc agtgagagag ttacaaattt aaaagagagg tttttcatct
      361 tttgtatttg ttccgacaat aataaaaata tggttcaatt tccctttttt gaaaagaggt
      421 atcgcttcgc ctcttgtgaa aagtatgttc ccttcatctt ctttcagaga gcttatgatc
      481 cctatcggta gatatggaat gctgtaattt tcactcagat tggcactcag cagctttttc
      541 ccgtcggcgg atggaatgtt gcagaccttg tcaagaaaca gtattgaaag cgactgcccg
      601 ttgcctttaa gtatcgctgt gcattcctct ctgttctcaa gaactgcggg aatagtaaac
      661 tgttttgatg ttataagttc aacttcagcc cttgatgtgt gaaagctgga caccagaccg
      721 acaagtccca tacttgagac cactgttgct ttttcagcca taagctttga atattcttga
      781 ggaaagctga ctactatcct gttggggttt atgtctgaga agttaagtat tctggcgggg
      841 attatcgtat attttccgta gctgtccttt aattttaata gttttgaaag ctcttcattt
      901 tcttttttga tagcttcatt cattatcaga cggactttga gttctgagat ctcgcgctca
      961 agttcaatat tcttctttcc cacatttttc agtccgataa tgatctgtac agtatttctt
     1021 acagtttcta tctgatgctg aaagaacgat ttcatgctgt cggctgcaac tgatctggga
     1081 gtgtattcaa gaataaaatt aggtattgcg aaagcaagta cgatggaaac cgccaggata
     1141 tatagaaaga ttttttttct gttcaaatca atacagtgaa atggttttca aaagtgaaat
     1201 attttcaagt attttgccgg ttcccataac aacagacttc aaaggatcat cggcaataat
     1261 tacgggaaga ttggtctctt cccttaaaag aatatcgagg tttttcaaaa gagcaccgcc
     1321 gcctgcaagg acgatccctt tgtcaacgat atctgcggca agttcggggg gagtgttctc
     1381 aagaacggtc ttgactgcgg aaacgatagc tttaacgacc tcttcaagcg cttcccttat
     1441 ttcgtcgctg ttgatctcga tcgtcttggg cactccggca ataaggtcaa gacccttaac
     1501 ttccatgaat tccttggttt catcagggta tgcattgccg atcgtccact tgatctgttc
     1561 agccattctt tcaccgatgg cgagattata tttctttttt atgtagtcaa tgatgctttc
     1621 atccattttg tcaccggccg attttaccga ttttgaataa acaatgccgg cgagcgagat
     1681 gacggcaact tctgttgtgc ctccgccgat gtcaactatc atgttccctg tcggttcagt
     1741 tacaggcatg ttcgccccta ctgccgccgc catcggctgc tcaatgagga aaacttctct
     1801 tgcaccggct ccgagcgccg cttccttaac agttcttttt tcaacttcag ttatgccgta
     1861 aggaactcct attgcgatcc tcggttttat gaagcttccg tttctgtatg catgtctgat
     1921 gaaatacctg agcatctggt gcgcaacttc aaaatctgca atgacaccgt ctttgagagg
     1981 ccttatgaca cttatgttta ccggcgcttt accgatcatt tcctttgctt ctctacccac
     2041 agccctgaca gattttgccc ctcttgaatc cttttcctgc acggcaacaa cgctcggctc
     2101 atttgctacg atgccgatcc ctttgacata tatcagcgta ttggcagttc caagatcaat
     2161 tgcaatgtcg cttgtaaata tcgataataa attgtcgatc ttaaacattt tcagtaatct
     2221 ctctaaatta agtttgaaca aaaaaaacgg attagagtat atgtgtttaa tgtagcaaag
     2281 taaagaagtt ttttatttca ttttgttgta agcaaatatc ttgccaaccc cttttttata
     2341 gtttataatg ccttcgaaat ttctttggtg gaaagatggc cccttcgata aaacttcaac
     2401 agagtctggt tcagaaacag gaactgaaaa tgaccgttca aatgaggcag agcatagaaa
     2461 tgcttctgaa accggcattt gagctcagag atatgatact tgccgaggcg gaagagaacc
     2521 ctttccttga gatcgaggag tggggggacg gaaacgacga tgatgttgtc catggaccgg
     2581 ttgagccggt tgaagagtcc ttgcaggaca atggggaccc ttcgcccttg aagatcgggg
     2641 atgaaaatgc cgatatgagt gatattcttg ctgaatttga ttgggatcag gtgagagaga
     2701 gctcatccaa cagcttcgga gagaccatcg tcaggaagaa gaatgacagc agcgatgatt
     2761 tcagttttga ggattttgtt cctgcaagcg aaagtttcaa ccagttcctt gattttcaga
     2821 tatgttcgct tcaggtcact tctgtgttga aagacataat gatatacatg acttacaatc
     2881 ttaacgaaaa aggttttctt gaagatagcg atgcaagcgt ggcagagatg accggatcgg
     2941 aagtgactga tgtggagaaa gcaaggcagc ttcttaaatt gatggatccg aaaggatgcg
     3001 ggtgtcatga tcttgttgac tatctgaggt ttatgttcac tgaagtgatg aaagatgatg
     3061 ttcctgacag gttctctctt tccgtccaga agctttttca tgatgaagat atgctggagc
     3121 ttctgataaa aaagaatttt gattcacttt gcgaaaaact ttcgcttgac aaggaagatc
     3181 ttactgagct tctccagttt ttcaggaaag g
//
LOCUS       DAOOYB010000317         3144 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_37366_length_3144_cov_11.573972, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000317 DAOOYB010000000
VERSION     DAOOYB010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37366_length_3144_cov_11.573972"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="PL056_12755"
     CDS             <1..792
                     /locus_tag="PL056_12755"
                     /inference="COORDINATES: protein motif:HMM:TIGR02232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47933.1"
                     /translation="GNTDNQDECTNECKDATCGDGFLWRNSDEMCDDGNDDNTDGCAE
                     CRLATCGDGYVQSGKEECDNGTGNNSDNAACTSECKNNICGDGKLLTGTEVCDGNQTC
                     SSLGKAYVNGLAICTNSCTTLDESGCDKKLPELKVEFTQRSSTYSERMYVEWQSHTGV
                     KCSDAMNSKSCMIPPVGEIWIRGDGSGYASPNYAYFRDFQNITGTFKAKVSSSYDCTV
                     DRVDIYVSGTKVKTVDTNKNCETSSPTFLMPAAGNVWDIDNFRWQ"
     gene            complement(899..2773)
                     /locus_tag="PL056_12760"
     CDS             complement(899..2773)
                     /locus_tag="PL056_12760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPM47934.1"
                     /translation="MDGDARRTRVDAILKVLSDKIFIIDSKGRFLDFLAKGNDDLALP
                     REKIVGSSIFDIFPPDEAERHLKIFKEVMNDGNLRMFEYSLTMDKTERYYEAEITKLS
                     DTEVISIIREITLRVKAEKALKESESRFRELADMLPEVVFETDVSMVVTYVNRKGIEL
                     FGYPDDYYPQNLKCIDLITPEERETGAQNIISKMKGDHNTPSEYTGVKKDGTTFPILL
                     HSVPYFKDGKPAGIRGIVVDLSLIKKVERDLANAIKLESLGLLAGGIAHDFNNLITGL
                     FGSVDIAKKEIENGNIKEAGEVLGNSLPAFERAKDLTNQLVTFAKGGEPKKKKGDLGK
                     IVTDTVKFALTGSEIVPEFHSDKEIYTAEFDPFQIGQVIENIIVNAKHAMSDKGILEI
                     GIDTVLMDDKGTELLAPGEYVRISIKDSGTGIPSEHRNNIFDPFFTTKKEGTGLGLAT
                     SWSIIKRHGGTIDFDSEKGRGTVFRIYIPSAECELESESSSKESFSGRGRILIMDDEF
                     IIREVAGKMLKDAGFTVDKAVSGEEAVDMYKEAFNKGIPYDVIILDLTIPGGMGGNKA
                     LQKIKEFHKDVAAICSSGYSEDDIISNPQKYGFAASLPKPYLRETFSEVIRKVISGKK
                     "
     gene            complement(2945..>3144)
                     /locus_tag="PL056_12765"
     CDS             complement(2945..>3144)
                     /locus_tag="PL056_12765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47935.1"
                     /translation="EMMPSLGKKIYNEGRQEGRQEGAFAKAVETCKKALKKGFDINTI
                     IELTGLPVEKIKEIQKTLQTD"
BASE COUNT          861 a          677 c          636 g          970 t
ORIGIN      
        1 ggaaacaccg ataatcagga tgaatgcaca aatgaatgca aagacgcaac atgcggcgac
       61 ggatttctgt ggagaaacag cgatgaaatg tgtgacgacg gaaatgatga caatacagac
      121 ggatgtgcag agtgcagact tgcaacatgc ggcgacggat atgtgcagtc aggaaaggaa
      181 gagtgtgaca acggcaccgg aaataacagc gacaatgcgg catgtacatc tgagtgcaag
      241 aacaacatat gcggagatgg caaattactc acaggaacag aggtttgcga cgggaatcag
      301 acctgttcat ctcttggaaa agcatatgtg aacggtcttg ctatctgcac caatagctgt
      361 acaacgcttg atgaaagcgg atgtgacaaa aaactgcctg aattaaaagt ggaatttacg
      421 caaagaagca gcacatattc agaaagaatg tatgtcgaat ggcagtctca taccggagta
      481 aagtgttctg atgcaatgaa ttcaaaatca tgcatgattc cccctgtcgg agagatctgg
      541 ataagaggag acgggtcggg ttatgcaagt ccgaactatg catatttcag ggattttcag
      601 aacattaccg gaactttcaa agccaaagtc agttcatcgt atgactgcac ggtcgacaga
      661 gttgatatat atgtcagcgg gacaaaagtt aaaactgttg ataccaataa aaactgtgaa
      721 acttcaagtc cgacatttct tatgcctgcc gccggaaatg tatgggacat tgacaatttc
      781 agatggcagt aaatgaaccc aaacggtttc ttgaaatata gttctgtcgt attgggatcc
      841 aggcatccgg tgacggtgaa aataaccctc gctcaaaaca caacgacacc ggatgccttt
      901 attttttccc tgatataact tttcttatca cttctgaaaa tgtttcacga agatagggtt
      961 tgggcagaga tgcggcaaat ccgtattttt gagggtttga tattatgtcg tcttcggaat
     1021 aaccgcttga gcatattgcg gcaacatctt tgtgaaattc ctttattttc tgaagagctt
     1081 tatttccacc cattccgccg ggaattgtaa gatcaagaat tatcacatca taaggaatgc
     1141 ctttgttgaa agcttcttta tacatatcaa ccgcctcttc tccgctcact gccttatcaa
     1201 ctgtaaatcc ggcatctttg agcattttgc ctgcgacttc cctgattatg aactcatcat
     1261 ccattatgag gatccttccg cgacctgaaa aactctcttt tgaagaagat tcactttcaa
     1321 gttcacattc agcggagggg atgtatattc ggaacactgt ccccctcccc ttttctgaat
     1381 caaaatctat cgttccacca tgccttttaa tgatagacca tgaagttgca agtccaagcc
     1441 cggtgccttc ctttttggtt gtgaagaacg ggtcgaatat gttgtttctg tgctctgacg
     1501 ggatccctgt gccggaatct tttattgaga tcctgacata ttcaccggga gcaagaagtt
     1561 ctgtgccttt gtcatccatc aaaacagtgt caataccaat ttcgaggatc cctttgtcag
     1621 acattgcatg ctttgcatta acaataatgt tttcaataac ctgccctatc tgaaacgggt
     1681 caaattctgc tgtgtatatt tctttatctg aatggaactc aggaacaatt tctgagccgg
     1741 tcagagcaaa tttgacagta tcggtgacaa ttttcccaag gtcgcctttc ttttttttgg
     1801 gttctccgcc ctttgcgaaa gtaaccagct gatttgtaag atcttttgcc ctttcaaatg
     1861 caggaagcga atttccaagc acttcgccgg cttctttaat atttccattt tctatttcct
     1921 tttttgctat gtctaccgaa ccgaaaagac cggttatcag attgttgaaa tcatgggcta
     1981 tcccccccgc cagaagtccg agtgattcaa gttttatcgc atttgccaga tcccgctcca
     2041 ctttttttat caaactcagg tcaacaacga tgcctcttat tcctgccggt tttccatctt
     2101 taaaataagg aacagaatgt aaaagtatgg ggaaagtcgt gccatctttt ttcacaccgg
     2161 tatattctga aggtgtatta tgatctcctt tcattttgct gataatgttc tgagcaccgg
     2221 tctctctttc ttcaggggtg ataagatcta tgcatttcag gttttgagga tagtaatcat
     2281 caggataacc gaacagttct atcccttttc tgttcacata tgtcacaacc attgacacat
     2341 ctgtttcaaa aacaacttca ggtagcatgt cggcaagctc tctgaacctg ctttcgctct
     2401 ccttcagtgc cttttcggcc ttgaccctca aagttatttc acgaattatt gagatgactt
     2461 cggtgtcgct gagctttgtt atctccgctt cataataccg ttcggtttta tccatggtga
     2521 gcgaatattc gaacattctc agatttccgt cattcatgac ctctttaaat attttcagat
     2581 gtctttctgc ctcatccgga gggaatatat cgaatattga tgatcccact atcttctctc
     2641 ttggaagtgc aagatcgtca ttaccctttg cgagaaaatc aaggaaacgg cctttggaat
     2701 caatgatgaa tatcttgtcg gaaagaactt tcaggatcgc atcaactctt gttctgcgtg
     2761 cgtcaccatc catttcaaac ctccggcaat gttcataata ttatcgcttt tttacaattt
     2821 ttaacaagaa aagagttttg gagagaatag tcagataaaa gtattaaaga ctggatgtga
     2881 actgattaca aaacttctgc tttttttggg gaggtccaat ggattttgtg ttctataatg
     2941 aaaattaatc tgtctgaaga gttttctgaa tttctttgat cttttccaca ggaagacctg
     3001 tcaattcaat gatggtattt atgtcaaatc cttttttcaa cgccttcttg caagtctcaa
     3061 cagctttggc gaaagcacct tcctgtctgc cttcctgtct gccttcgttg tatattttct
     3121 ttccaagtga cggcatcatt tcct
//
LOCUS       DAOOYB010000318         3141 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_37415_length_3141_cov_13.729099, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000318 DAOOYB010000000
VERSION     DAOOYB010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37415_length_3141_cov_13.729099"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            79..663
                     /locus_tag="PL056_12770"
     CDS             79..663
                     /locus_tag="PL056_12770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47936.1"
                     /translation="MKKILVFLTAIFMFAGLFGQEIKIDEKTAEDLNRLAEAYSSLGI
                     EMEMILADPDKISKDEAMSIDEVIANIEGLIQIKSLPADCEGNLAVLKIYERVTTPSG
                     FIYAWLGKISSYLGSMGDWDGSMHWLVTSGGQSSSIKTVSGPSVQYNSSEYSGYRWAK
                     ARFIENGLVYVPGIGYVIMPINFGPIKCYDLNNL"
     gene            732..>3141
                     /locus_tag="PL056_12775"
     CDS             732..>3141
                     /locus_tag="PL056_12775"
                     /inference="COORDINATES: protein motif:HMM:NF012632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47937.1"
                     /translation="MNTFFRIFLIFLMFFLFSCEKEKLVRDDEALNDDVIENDENMSD
                     DDQIFACADEWEKTELIEDVSTMFDFDIKVETGKWNWEKKEEFDELVFEGDPSRVLGV
                     LNDGSLILSDYYNTLEGYSILAVLHPDGTTKRYILEIDKGKKITFGIGTIDAYKPFVF
                     WPGYYFDKDRQEILISVTMMTKLESPYSDEYSARPFVIKIDSTGGLSYISWENDGTSI
                     CSDLTQTDDKIVLFCSHWIPDPEIYMKEGMINAEINVINGNTVYRKMMKSEYIALTKN
                     IAGSKNGEIYSFYEESTFNETETTDAGFIYSFDEKDLCLKTEGPENYFDELAFDGSPY
                     EGYNMMYPFSFLKKGEYSIVGGVRTKLEHLDYEQIQSFNPSLYVDHSDNDTIFSFGIK
                     DPRFEVGEVSGNHPVAEPAIFSKENSNLLFVSLISTFDMEDEGQDWLTPELLEKTDFL
                     YRPSIAAVDIEKKEVYIRNFFMGDLVGFFGEAFSFEEYIYFVVNYGQERTLYRMPETW
                     LINENARAKESRFFIEDIAKLREAVLKELKPFETVSSGKKHTCAVDEEKRVICWGDNS
                     ENQLGNQIGLTRSYPDLIFMDGILKDKTVEIMKTGSAHACVVADDKRIYCWGSNDNGK
                     LGKGGGIESFYPVSVKYDGALKDKKIADVAPGTVHTCAIDVAGKAYCWGRNTLGSVGT
                     ETDLQFVYEPVSVDMTGVLKDRKIIEISSGSAHTCAVADDGGVYCWGSNGRGQLGDGN
                     GGNEGSLTELGDFSIVPVKSETAEGKYVKVSSGSSHTCILSDAGKIYCFGDNKNGQLG
                     DGTTE"
BASE COUNT         1006 a          469 c          739 g          927 t
ORIGIN      
        1 ccccttaaaa attagtcggg aaaacggttc atttattttt gaaaagatgt ttttttaact
       61 aaacttaagg agttggagat gaagaaaata ttggtttttt tgactgcgat atttatgttt
      121 gcaggtcttt ttggacagga aataaaaatt gatgaaaaga ctgctgaaga cctcaacaga
      181 cttgcagaag catatagttc tttaggaatt gaaatggaaa tgattcttgc agatccggat
      241 aagataagca aagatgaagc aatgtccatt gacgaagtaa ttgcgaatat tgaaggactt
      301 attcagatta aatctctccc agccgattgc gaaggaaact tagcggtttt aaaaatatat
      361 gagagagtaa cgactccaag cggatttata tacgcttggc ttggtaagat ttcttcttat
      421 cttggctcta tgggagattg ggatggttct atgcactggt tggtaacaag tggcggacag
      481 agcagcagca ttaaaacagt ttccggacct tcggttcagt ataacagtag tgaatatagt
      541 ggttacagat gggcaaaagc gagatttatt gaaaacgggc ttgtttatgt tccgggaatt
      601 ggttatgtaa ttatgcctat taatttcggt ccaattaaat gttacgacct taataattta
      661 taaattgtta aggaaggttg aaataggggc ggagaaaact ctgcccctta tatttttcag
      721 gaggttccct gatgaacact ttttttagaa tttttctgat ttttctaatg ttttttctgt
      781 tttcctgtga aaaggaaaag ctcgtcagag atgatgaggc tcttaatgat gatgtaattg
      841 aaaatgatga aaatatgtct gatgatgatc agatttttgc ctgtgctgat gaatgggaga
      901 agacggaact gatcgaagat gtgagcacga tgtttgattt tgatataaag gtggagaccg
      961 ggaagtggaa ctgggaaaaa aaggaagaat ttgatgaact tgtgtttgaa ggagatccct
     1021 caagagttct tggtgtcctg aatgacggaa gtctgatcct gagtgattat tataatactc
     1081 tggaaggata ttcgatcctt gcagttctgc accctgacgg aacaacgaag cggtatattc
     1141 ttgaaattga caagggaaag aaaatcactt tcggcatcgg aacaattgat gcatacaaac
     1201 cgtttgtttt ctggccgggc tattattttg ataaagatcg gcaggaaatc cttatttctg
     1261 ttacaatgat gacaaaactt gaatctccat acagcgatga atattcagca agaccgtttg
     1321 taattaaaat agactcaaca ggcggtctgt catatatttc atgggaaaac gacggaacaa
     1381 gcatctgctc tgatctgacc cagactgatg ataaaattgt ccttttctgc tcacactgga
     1441 ttcctgatcc tgaaatatat atgaaagaag gcatgataaa tgccgaaata aatgtaataa
     1501 acggaaacac tgtttacaga aaaatgatga agtctgaata tattgcgttg acaaaaaaca
     1561 ttgcaggcag caaaaatggt gagatatatt cattttacga agaaagtaca ttcaatgaaa
     1621 ctgaaacaac ggatgccggt tttatctaca gttttgatga aaaagacctc tgccttaaaa
     1681 cggaaggacc tgagaattat tttgatgaac tggcttttga cggttcccca tacgaaggat
     1741 ataatatgat gtatcctttt agttttctta aaaaaggaga atattccatt gtcgggggcg
     1801 tcagaacgaa acttgaacac cttgattatg aacagatcca aagctttaat ccgtcattat
     1861 atgttgacca ttctgacaat gacacaattt tttctttcgg aataaaagat ccgagatttg
     1921 aggttggaga agtttcggga aatcaccctg ttgccgaacc agccattttc agtaaagaaa
     1981 acagcaattt attgtttgtg tcattgatat cgacttttga tatggaagat gaaggtcagg
     2041 actggctaac tcctgaactt cttgaaaaaa ctgattttct ttaccggcct tcaattgccg
     2101 cagttgatat tgagaaaaaa gaggtataca tcaggaattt ctttatggga gatcttgtcg
     2161 gcttttttgg agaggcgttt tcatttgaag agtatattta ttttgtagta aattatggtc
     2221 aggagcggac tctgtaccgg atgcccgaaa catggcttat aaatgaaaat gcaagagcga
     2281 aagagagccg cttctttatt gaagatattg caaaattgcg ggaagctgta ttgaaagagc
     2341 tcaaaccgtt tgaaacggtt tcttcaggga aaaagcacac atgtgcagtg gatgaagaaa
     2401 aaagggtgat ctgctgggga gataattcag aaaatcagct tggaaatcag attgggctca
     2461 caaggtctta tcctgacctt atatttatgg acggaattct taaggataaa acagttgaaa
     2521 ttatgaaaac aggatctgcc catgcatgtg ttgttgcgga tgataaaaga atttactgtt
     2581 ggggcagtaa cgataacgga aaacttggaa aaggtggtgg tatagaaagt ttttatcctg
     2641 tatcagtaaa atatgatgga gctttgaaag ataagaaaat tgcagatgtt gctcccggaa
     2701 cagttcatac ctgtgcaata gatgttgccg gaaaagccta ctgctggggc agaaacactc
     2761 ttggcagtgt tggaactgaa acggatcttc agtttgttta tgaacctgtt tctgttgaca
     2821 tgactggagt tttaaaagat agaaaaatta tagaaatatc aagcggttct gcgcatacat
     2881 gtgcagttgc cgatgatggc ggggtttact gctggggatc aaatggcaga ggacagcttg
     2941 gagatggaaa cggaggaaat gaaggaagtt taacggagct tggagatttt agcattgttc
     3001 ctgttaaatc tgaaacagcg gaaggaaaat atgtaaaggt ttcatccggt tcatctcaca
     3061 catgtattct gagtgatgca gggaagatat actgtttcgg agacaataag aacggtcagc
     3121 ttggagacgg gacgactgag a
//
LOCUS       DAOOYB010000319         3131 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_37590_length_3131_cov_7.592978, whole genome shotgun sequence.
ACCESSION   DAOOYB010000319 DAOOYB010000000
VERSION     DAOOYB010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37590_length_3131_cov_7.592978"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="PL056_12780"
     CDS             <1..434
                     /locus_tag="PL056_12780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47938.1"
                     /translation="AGLHIRDVETLNLKAYEYAECYTGELTTDHGGFYLSCDGYEYRF
                     EGRPDTYFNFYPLDGDIREMQENHVYNGWTYIPDGSNVKLSTLNDVPSYCGNGVIEEG
                     ELCDGNSVACTTLDPDYTGGSAACNSTCNGYNESNCSTDGW"
     gene            437..1984
                     /locus_tag="PL056_12785"
     CDS             437..1984
                     /locus_tag="PL056_12785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47939.1"
                     /translation="MRNTLIILILMALSACNSDKPKNDVDINDKDSGLTDIDTPVPDP
                     DISDNLSDEVSTESDEQPDSDTDTNSVECLDLRYNENTIKTNFPLKDKDGKPTFCRPG
                     CDTPTETDPQCVRNIWEWDNWEEYQVYLEAEKKDPNQYEERECYPWPCKLPDMKASVN
                     PQSFNSKCDRKLTVNGFKANIGTVWTHGMSDSVAGMDFTHTGRVTEYDPEKDEYFNLG
                     HVKGHLNYNEGRYVVLVSDSIPDKNSDYKAFVISILRKDEKYYYELIYDNDNHNVFMQ
                     RPPFSGKNWVLIQVKSGINGSKIEVKYARAGIWEWQSIGEIGDPLAQEGNIVGDHLTF
                     ITNNREIYYCDLRKYPKHINDCLKINRLNTDKITYEQGNSPKIDTENEYRLVYNVDST
                     QIFVEVDLADINNPEYTEHEIPKKKELFAGFSVDTVRGNKVVYGEMFDMGSGTPDFLG
                     CFYRFDKKKSYCPQENIHPWTAENLMGYNTFWGKWHLWKNPASVGAYMRDWECYCKES
                     GVCPFEE"
     gene            2221..2793
                     /locus_tag="PL056_12790"
     CDS             2221..2793
                     /locus_tag="PL056_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HPM47940.1"
                     /translation="MTIKGTRTEQNLLKSFAGESQARMRYTYWAKQAKKDGYEKVAAI
                     FEETANQEKEHAKRMFKFLEGGMVEITAMYPAGKIGTTLENLRDAAGGEHEEWTELYP
                     EFARIADEEGFKDVAVMYRMISNAEKHHEARYRQLLEKIEKNEAFKSEENVEWRCLNC
                     GYIHNGKNAPEKCPACEHPQAYFEINNTNW"
     gene            2801..>3131
                     /locus_tag="PL056_12795"
     CDS             2801..>3131
                     /locus_tag="PL056_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HPM47941.1"
                     /translation="MMKRRDIFKCEICGNIVGVAHVGGGELVCCGKPMAFMEEKNADS
                     STEKHVPVIEATENGVKVTVGSTLHPMVDAHYIEWIEVINGEYNQRKYLKPGDQPVAE
                     FFVPYSDK"
BASE COUNT         1056 a          537 c          730 g          808 t
ORIGIN      
        1 gtgcgggact ccatatcagg gatgttgaga cacttaatct taaagcgtat gaatatgcgg
       61 aatgctacac aggtgaactt accacagatc acggtggctt ttatctctcc tgtgacggat
      121 atgaataccg ttttgaggga agacctgata catattttaa tttctatccg cttgacggcg
      181 acatccgtga aatgcaggaa aaccatgttt acaacggctg gacatacatc cccgacggaa
      241 gtaatgtaaa actttcaaca ctcaatgatg ttccgagcta ttgcggaaac ggagtgatag
      301 aggaaggtga gctctgtgac ggaaattcag ttgcatgtac gacacttgat cctgattaca
      361 caggcggctc agccgcttgc aattcgacct gtaacggcta taatgaaagc aactgttcaa
      421 cggatggatg gtaaacatgc gtaatacact aattatattg attttaatgg cactttcggc
      481 ttgcaactca gataagccga aaaatgatgt tgatattaat gataaggatt cgggacttac
      541 agacattgac acccctgttc ctgatcccga catttctgat aacttgtccg acgaagtttc
      601 aacggagtcg gatgaacagc ccgactctga tacagacacg aattcagtcg aatgtcttga
      661 tctcaggtat aacgaaaaca cgatcaaaac aaatttcccg ctcaaagaca aggacggaaa
      721 gccgacattc tgccgtcccg gatgcgatac acctactgaa acagaccctc aatgtgtgag
      781 aaacatctgg gaatgggaca actgggaaga atatcaggtt taccttgaag cggaaaaaaa
      841 agatccaaat caatacgaag aaagagagtg ttatccttgg ccgtgcaaac tgcctgatat
      901 gaaggcatcg gtaaatcctc aaagttttaa cagtaaatgt gatagaaaat tgactgttaa
      961 tggttttaaa gcaaatatag gtacggtgtg gacacacgga atgagtgata gcgttgccgg
     1021 aatggatttt acacatacgg gcagagtcac tgagtatgat cctgaaaaag atgaatattt
     1081 caatctcgga catgtaaaag gacatctcaa ctacaatgaa ggaagatatg ttgttcttgt
     1141 gtcagattcc attccggata aaaattctga ttataaagct ttcgtcattt caattttaag
     1201 aaaagatgaa aaatattatt atgaattaat ttacgataac gataatcaca atgtttttat
     1261 gcagcgacct cctttttcgg gaaagaactg ggttcttatt caagtaaaat cgggtattaa
     1321 cggttctaaa atagaagtga aatatgccag ggcgggaatt tgggaatggc agagtattgg
     1381 agagattggt gacccacttg ctcaggaagg aaacatagtc ggagatcacc tgacatttat
     1441 tacaaacaac cgtgaaatat attactgtga cctgagaaag tatccaaaac acattaacga
     1501 ttgcttaaag ataaaccgcc tgaacactga caaaataact tatgaacagg ggaattcgcc
     1561 taaaattgat acggaaaatg aatatagatt ggtttacaat gtcgattcaa ctcagatatt
     1621 cgtggaggtg gatctcgccg atataaataa tcctgaatac accgaacatg aaattcctaa
     1681 aaagaaagag ctgtttgccg gatttagtgt ggatactgtg agaggaaaca aggtagtgta
     1741 cggagaaatg ttcgatatgg gatcgggaac gcctgatttt ttaggatgtt tctacagatt
     1801 tgacaagaag aagtcatact gcccacagga aaatattcac ccatggacag ccgagaatct
     1861 tatggggtat aacaccttct ggggcaaatg gcatttatgg aaaaatccgg catcagttgg
     1921 tgcatatatg cgggactggg aatgttactg taaggaatcc ggggtctgtc cgtttgagga
     1981 gtaaatgcgt aaaatttggt tcagaaagat ttaattttat aaatagaaaa tccttgatat
     2041 aatcaaaacc ggtcttataa aatagtttcg tgcgatgatc ttttataaaa gatttagcag
     2101 gtttttaatt cggacagtgt tgactttaat agggagatgt gataaaaact cgtgatgatt
     2161 gaatttagga tgtttaacat atcagatttt tttactaatc aatgaaaatc ggaggaaatg
     2221 atgacaatta aaggaaccag aactgagcag aaccttctta aatcttttgc cggtgaatct
     2281 caggcaagaa tgcgttacac ctactgggca aaacaggcaa aaaaggacgg ttatgaaaag
     2341 gttgccgcaa tttttgaaga gacggcaaat caggaaaagg aacatgcaaa aagaatgttc
     2401 aaattccttg aagggggaat ggttgagatc actgcaatgt atccggcagg aaaaattgga
     2461 acaacacttg aaaacttaag agatgctgcc ggtggtgaac acgaagagtg gactgaactt
     2521 tatccggaat ttgcaagaat tgccgatgaa gagggtttca aggatgttgc cgttatgtac
     2581 agaatgatct caaatgctga aaagcatcac gaagcaagat acaggcaact tcttgaaaaa
     2641 attgagaaaa atgaagcatt taaaagtgag gagaatgttg agtggcgctg cctgaactgc
     2701 ggttacattc ataatggtaa aaatgctccg gaaaaatgtc cggcatgtga acatcctcag
     2761 gcgtattttg aaataaacaa cacaaactgg taggagaaaa atcatgaaaa gaagagatat
     2821 ttttaagtgt gagatctgcg gaaacatagt aggcgttgca catgtcggcg gcggagaact
     2881 ggtatgctgc ggtaagccga tggctttcat ggaagaaaag aacgctgaca gctcaactga
     2941 gaagcatgtt cctgtcattg aagcaaccga gaacggtgtg aaggttacag tcggttcaac
     3001 acttcatccg atggttgatg cacattacat tgagtggatc gaagtgatca acggtgaata
     3061 caaccagaga aaatatctta aaccgggtga ccagccggtt gcagagtttt ttgttcctta
     3121 cagcgacaaa c
//
LOCUS       DAOOYB010000320         3112 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_37947_length_3112_cov_4.068695, whole genome shotgun sequence.
ACCESSION   DAOOYB010000320 DAOOYB010000000
VERSION     DAOOYB010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37947_length_3112_cov_4.068695"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1119
                     /locus_tag="PL056_12800"
     CDS             <1..1119
                     /locus_tag="PL056_12800"
                     /inference="COORDINATES: protein motif:HMM:NF019194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HPM47942.1"
                     /translation="PMSQEAFFNESNRVHAEYLAGSSLELEKLANTYLGKGEHLPDTL
                     QKASGMMFAVTKNFLSATQAIFPLTLFLLIVFIIILRERLGKKDEIFFGIFISIIGMG
                     LFNFGIEFGLAKMGTQVGKRLPSSFSAIEIPEDTEIIKNFNPEIVNKSIDSEGNVSSF
                     FYKRSENHYVATPFYEENFEQSSSTYRYVPKKGPLFGNEGSAWGFVVVLLFAFIMGYG
                     ATLAEPALNALGMKVEELTVGTFKKSLLMHTVATGVGVGIAVGAAKIIWDIPLIWLLV
                     PPYLILLIITRFSSEEFVNIGWDSAGVTTGPVTVPLVLAMGLGIGTQVGVVEGFGILS
                     LASVYPILGVLLVGLYTIRKSKKLLLSNNDVNEMEGEL"
     gene            1116..2228
                     /locus_tag="PL056_12805"
     CDS             1116..2228
                     /locus_tag="PL056_12805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47943.1"
                     /translation="MIVRNISKFTCSVYRFVSPLVAETLKRCSIDEYFLQPAKALVSV
                     DRRRFLLSGGGYMENIMDTYHFSCETEREVYVFEQIVKDLSLDQEGRGSIYVEPAVII
                     DHNDNTESSLNFTDLDVGSFDTKLMGIYCIIQRGRGDIIAKTAIDTGSSVPAIYYGVG
                     GGIRDRMGLIRITIPADKEMANIIVTEHDCIGMMNILIDAGSLDRPGMGFIFNYPVTR
                     AVTDTRTYIGKSRSAASIGQIVKAIDGIKGNTEWRKRIINPLNIKERNFLKDLDNITL
                     ICDDTKAKDMLDVAMCAGATGATLSKCRRSFLSKENGMLNKKSVEMADMVVSTNQKSE
                     IIQALINAGFFSSEINGKIMIRKSTLAFSYSDNGKS"
     gene            2325..2699
                     /locus_tag="PL056_12810"
     CDS             2325..2699
                     /locus_tag="PL056_12810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47944.1"
                     /translation="MRFTTLLLIVSIFTFVFDGKELCFKNNGEVHLSCCCKDSPSIKM
                     PCCGNNCKTVDVPSIDIAQHKPENENSLNPKSDNSFFVSFYNNVSVSILLKVPVFSPI
                     YRSDHSYRSRPLSNLSTIVLRC"
     gene            2755..>3112
                     /locus_tag="PL056_12815"
     CDS             2755..>3112
                     /locus_tag="PL056_12815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47945.1"
                     /translation="MTKLLKISIVMLAFALLSTGYIFAKEKCCDKEGKSSEVTKCDCK
                     GCENCKCGCKEGKECKCKDCKDCKCGCKGDKECDCKKDKNCDCKKSDCKKGEKSGCGC
                     KDKDAKKGHDDCPHKKQ"
BASE COUNT          994 a          561 c          665 g          892 t
ORIGIN      
        1 cctatgtcac aagaggcctt cttcaatgaa agtaaccgtg tccatgcaga ataccttgcc
       61 ggatcttctc tggaattaga aaaacttgct aacacctact tagggaaagg agaacatctc
      121 cccgacactc ttcagaaagc ttcgggtatg atgtttgctg tcacaaagaa ttttctgtct
      181 gcaacacagg ccatattccc tctgacactg ttccttctga tagtattcat catcattctc
      241 agagaacggc taggcaaaaa ggacgaaata ttcttcggga tattcatttc cattatcgga
      301 atgggacttt tcaattttgg aattgagttc ggtcttgcaa aaatggggac tcaggtcggc
      361 aaaagactcc cttcatcttt cagtgcgata gaaattcctg aagacaccga aataattaag
      421 aattttaatc cggaaattgt gaacaaatcg attgacagcg aaggaaatgt ctcctctttc
      481 ttctacaaaa gaagcgaaaa tcactatgtg gcaacacctt tttacgaaga gaattttgaa
      541 cagtcatcgt caacatacag atatgtcccc aaaaaaggtc cactattcgg gaatgaagga
      601 agtgcctggg gttttgttgt tgtcctcctt ttcgctttta taatgggtta cggggcaact
      661 cttgccgaac ctgcacttaa cgcccttgga atgaaggtgg aagaactcac agtaggaaca
      721 ttcaaaaaat cacttttaat gcatactgtt gctacaggtg tcggtgtcgg aatagctgtc
      781 ggagcggcaa aaataatatg ggatattccg ttgatctggc ttttagtacc gccttatcta
      841 atacttctta taattacaag gttttcaagt gaagaatttg taaatatcgg ctgggacagt
      901 gcaggagtta cgacagggcc ggtaaccgtt cctctggttc ttgcgatggg actaggcata
      961 ggaacacagg tcggtgtcgt tgaaggattc gggatactca gtctggcttc agtatacccg
     1021 atactcggtg ttcttctcgt cgggctttac actatcagga aatcaaagaa acttcttctt
     1081 tcgaataatg atgtcaacga gatggagggt gaattatgat agtaaggaac atttcaaaat
     1141 tcacctgcag tgtttaccgc tttgtttctc cgcttgttgc ggaaacgctc aaaagatgtt
     1201 ctatagatga atattttctc cagcctgcaa aagctcttgt ttcagttgac agaagaagat
     1261 ttctgctttc gggtggcggc tatatggaaa acataatgga cacttatcat ttttcatgcg
     1321 aaacggaaag agaggtttat gtttttgaac agatagttaa ggatctctct cttgatcagg
     1381 aaggacgggg atctatttat gttgaacctg ctgttataat tgatcataat gacaacacgg
     1441 aaagcagttt aaattttaca gacctggatg tcggcagttt tgacacaaaa cttatgggta
     1501 tctactgcat tattcaaaga ggacgaggag acatcatcgc aaaaactgca atagataccg
     1561 gttcatcagt tcctgcgatt tattacggtg taggtggagg cataagagac agaatgggtc
     1621 ttataagaat aaccatccct gcagataaag aaatggctaa cattattgtt acagaacatg
     1681 actgcatagg aatgatgaac attcttattg atgcagggag ccttgacaga ccgggaatgg
     1741 gtttcatttt caattatcct gttaccagag cggtaacaga caccagaacc tatattggga
     1801 aaagcaggtc cgctgcaagt attggacaga tcgtaaaagc tattgacgga atcaagggga
     1861 acaccgaatg gagaaaaaga atcatcaatc cactgaatat aaaagaaaga aactttctta
     1921 aggatctcga caacataacc ctgatctgtg atgacacaaa ggcaaaagac atgcttgatg
     1981 tggcaatgtg tgcaggtgca acaggagcca cactttcaaa atgcagaaga agttttcttt
     2041 caaaagaaaa tggtatgctt aacaaaaaat cagttgaaat ggctgatatg gtagtttcta
     2101 caaatcagaa atcagaaata atccaagctc ttatcaacgc aggatttttc agtagtgaaa
     2161 ttaacggaaa gataatgata cggaaaagca ctctggcctt ttcctattct gacaacggaa
     2221 aatcttaatc aatttcggag tagctggtat tattttatat ttcccctctt gacttaaggt
     2281 gcaaagacat ttaaaattgg taataggttt ttttggagat tctgatgaga tttacgactc
     2341 tgcttcttat cgtttcaatt ttcacttttg tttttgacgg gaaagagctt tgttttaaaa
     2401 ataatggcga agtgcatctt tcgtgctgtt gcaaagacag tccgtccatc aaaatgccct
     2461 gctgcggcaa caactgcaaa actgttgatg ttccttctat tgatattgct cagcataaac
     2521 ctgaaaacga aaattcactg aatccaaaat cagacaattc attttttgtt tccttctata
     2581 ataatgtaag tgtttcaatt cttttgaaag ttccggtttt cagcccgata tacaggtcgg
     2641 atcattcata cagatcaaga cctttatcta atctttcaac cattgtccta aggtgttgat
     2701 cttatctgca aatgcaactt tatttaaata acttaaactt ttatggagat tacaatgact
     2761 aaattattaa agatttcgat tgttatgctg gcgtttgcac ttctttcaac cggttatatt
     2821 tttgcaaaag agaaatgttg tgacaaagaa ggtaaaagtt ctgaagtaac aaagtgcgac
     2881 tgcaaaggat gtgaaaactg caaatgcgga tgtaaggaag gaaaagagtg caaatgcaaa
     2941 gattgtaagg actgtaagtg tggatgcaaa ggtgacaaag aatgtgactg caaaaaagat
     3001 aagaactgtg actgcaaaaa aagtgactgc aaaaaagggg aaaaaagcgg ttgcggatgt
     3061 aaggacaagg atgcaaaaaa aggtcacgat gactgtccgc acaaaaaaca gc
//
LOCUS       DAOOYB010000321         3101 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_38146_length_3101_cov_3.838148, whole genome shotgun sequence.
ACCESSION   DAOOYB010000321 DAOOYB010000000
VERSION     DAOOYB010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38146_length_3101_cov_3.838148"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3101)
                     /locus_tag="PL056_12820"
     CDS             complement(<1..>3101)
                     /locus_tag="PL056_12820"
                     /inference="COORDINATES: protein motif:HMM:NF012632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47946.1"
                     /translation="DGTNENRPLPVRILAQEDLGFVRISTRYEHTCAINSLGELYCWG
                     INDLGCLGDGTDVNRRYPVKIGEDIGPWIDISAGGAHTCAINSNGELYCWGLNSRGQL
                     GNNSHVFKSLSPVKVNEETDWKIIATGEIHTCSIKENGKLYCWGGNDYGEVGDGNICP
                     DDFDFCADRYSPVHISSDYKWSYISIGYQNNAAKREDGKFFVWGKNDYYSLGDGTDID
                     QPFPVEMKNKWETVVMGGSWNYGLHTCAIDEQKDLYCWGINLSGQLGIGTTTDSAKPV
                     KVEIPEQTEDKIDPDSPDNNSDKNDLDNNPDDEVNDSEPQPLCIPNPCTGKNKNVCQE
                     VYDLVNGFEMIYKCFCNDGWIGKDCNKCQKGYYGKSCELCNCKKNEICNDGLDGDGSC
                     ICATEIVEEKCICYPGWSGENCEIFGENWSCTPAGESCTTSGDCCFDGICMKSFLNDE
                     TLCRKTLRYGDEEIRELAVSPDSSIVATARMGSVRLHRVDNLKELFRVSRSVQASTAI
                     DISPDGVFLAVNDGGTLKLISMEDGTVIDSAYLSYNKKLKFSPDGKMLAAAHNTGFTV
                     YDVTNDSLNDYFSDSLPGNSYGWNVDSVDFSPDSNFLIAGSGLSLYDQNAVIYDLVEK
                     KVFQNLPINEDYVEVAFSQSGNMLATGGYHDGYENNVTVFKFVNNNWVFSYSLGGFSE
                     EVHSLAFNHSEDFLAIGGRSFAGVYSLYDKFPLLLSGGWQVKFTPDDKNVIFGRETGE
                     LEKLSLPAFDKPFSSNDNGSRLTQLSADGLKAAFSKGADTVVFAESLTGNVLNEVILP
                     FYIYSGKASFSSDLRAGAFSLDFNDDGGFVLFDFNKGSIVTEKKTGKYYNFAFSPNEK
                     TVAVLSWYKTKEISFYSTETGILIHNIPLSDDETVSGFHFSPDGSIAGAVLKNKVQFF
                     KTSDWSEIFSLPSGSVSFFPDGSKIILANGTVSVFSSLDGSEIEFPGLNINADIAVIS
                     ADEKYLLTESGILEIETGRTVSFASHYYGITGVGISNNNLIVTTQGKDSLRVIDAKEM
                     LEK"
BASE COUNT          877 a          688 c          580 g          956 t
ORIGIN      
        1 tatttttcaa gcatctcttt tgcatcaata acccttaaac tgtcctttcc ctgggtcgtt
       61 acgatcagat tattgtttga tataccgact cctgtaattc catagtaatg agaagcaaag
      121 gatacagtgc gaccggtctc tatctcaaga attcctgatt ctgtcagaag atatttctca
      181 tcagctgaaa taactgcgat atcagcatta atgttcaagc cggggaattc aatttcagac
      241 ccgtcaagcg aagaaaaaac agaaactgtt ccgtttgcca gaattatttt ggagccatcg
      301 gggaaaaaag aaactgatcc tgaaggaagc gaaaaaattt cactccagtc agaagtttta
      361 aaaaactgca ctttattttt taacaccgca cctgcaattg atccatcagg tgaaaaatga
      421 aatcctgaaa cagtttcgtc atcgcttaga ggaatattgt gaattaaaat gcccgtttct
      481 gtcgaataaa acgaaatctc ttttgtttta taccatgaaa gtacagccac ggttttttca
      541 tttggtgaga atgcaaaatt gtaatatttg ccggttttct tttctgtaac aatgcttcct
      601 ttgttgaaat caaacagtac aaagccacca tcatcattaa aatccaaaga aaatgctcct
      661 gcccgtaagt cactggaaaa acttgccttt ccggaatata tgtaaaaagg gagaatcact
      721 tcatttaaaa catttcctgt aagtgattcg gcaaaaacca cagtatcagc cccttttgaa
      781 aatgccgctt tcaatccgtc tgctgaaagc tgtgtaaggc gactcccgtt atcgttgctt
      841 gaaaaaggtt tgtcaaaggc gggaagtgac agtttttcca gctcaccggt ttctcttcca
      901 aatatcacat tcttgtcatc gggggtgaat ttgacctgcc atcccccgga aagcagtaaa
      961 ggaaatttgt catataaact gtaaacaccg gcaaaacttc ttccaccaat tgccagaaaa
     1021 tcttcgctgt ggttaaacgc aagagaatga acttcttctg aaaagccgcc aagagaatag
     1081 ctgaaaaccc aattgttatt aacaaactta aaaacagtta cattattttc gtatccatcg
     1141 tgatatccac ctgttgcgag catgttgccg ctttgtgaga aggcaacttc gacataatct
     1201 tcattaatgg gaagattctg gaaaaccttc ttttcaacaa ggtcataaat cacggcattc
     1261 tgatcataaa gtgaaagtcc tgagccggcg ataagaaaat tgctgtcggg agagaagtca
     1321 acagagtcaa cattccatcc gtatgaattc ccgggcagag aatcggaaaa ataatcattt
     1381 aaagaatcat ttgtgacatc ataaacagta aatccggtat tgtgtgcagc ggcaagcatt
     1441 ttcccgtccg gtgagaattt cagttttttg ttgtaactca gatacgctga atctataact
     1501 gttccgtctt ccatgcttat aagtttcagt gttcctccat cgtttactgc aagaaaaacg
     1561 ccgtcaggag aaatgtctat ggctgttgat gcctgaacag aacgggaaac tctgaacagt
     1621 tctttgagat tatcaactct atgcagtctg acggaaccca tcctcgccgt agccacaatt
     1681 gaactgtcgg gagaaacagc gagctctctt atctcctcat ctccgtatct gagtgtttta
     1741 cggcataaag tttcatcgtt taaaaaagat ttcatgcaaa ttccgtcaaa acagcagtca
     1801 ccgcttgttg tgcaggattc tccagcgggt gtgcatgacc agttctcacc gaatatttcg
     1861 cagttttcgc cactccatcc cggatagcag atacatttct cttcaacaat ttctgtggca
     1921 cagatgcatg atccgtcacc gtcaagtcca tcattgcaaa tctcattctt tttgcagttg
     1981 cagagttcac agctttttcc ataatatcct ttctgacatt tattgcagtc ttttcctatc
     2041 catccatcat tgcagaagca tttatagatc atttcaaaac cgttaacaag atcatagacc
     2101 tcttgacata catttttgtt tttacctgtg cagggatttg gaatgcagag aggttgcggt
     2161 tcggaatcat tgacttcatc atcgggatta ttatcaaggt catttttgtc agaattgttg
     2221 tcaggggaat cagggtcgat tttgtcttct gtctgttcag gtatttcaac ttttaccggt
     2281 tttgcactgt cagttgttgt tccgatacca agctgtccgc tgagattaat tccccagcag
     2341 taaagatctt tctgctcgtc aatggcacag gtgtgtaaac cgtaattcca tgagccgccc
     2401 attacaacag tttcccattt gtttttcatt tctacaggaa aaggttggtc aatgtctgtg
     2461 ccatctccaa gcgaatagta atcgtttttc ccccagacaa aaaatttacc atcttccctt
     2521 tttgcggcat tgttttgata accaattgaa atatatgacc atttgtagtc gctggaaatg
     2581 tgtacaggtg aatatctgtc tgcacagaaa tcaaagtcat ccggacagat gttcccgtct
     2641 ccgacttctc cgtaatcatt ccctccccag cagtaaagtt tgccgttttc ctttattgag
     2701 caggtatgaa tttcgccagt cgcaattatt ttccagtctg tttcttcatt cactttcaca
     2761 ggagacaaac ttttgaaaac atgtgaattg ttcccaagct ggcctcttga attaagtccc
     2821 cagcagtaaa gttcaccgtt tgaatttatt gcgcaagtat gtgctcctcc ggctgaaata
     2881 tcaatccacg gaccgatatc ttcaccgatc ttcaccggat atcttctgtt gacatctgtt
     2941 ccgtcaccaa gacagcccag atcatttatg ccccagcagt aaagttcacc aagtgaattt
     3001 attgcacatg tgtgttcgta tctggttgat attcttacaa atccgagatc ttcttgagca
     3061 agtattctta caggaagagg cctgttctca tttgttccgt c
//
LOCUS       DAOOYB010000322         3067 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_38800_length_3067_cov_6.457835, whole genome shotgun sequence.
ACCESSION   DAOOYB010000322 DAOOYB010000000
VERSION     DAOOYB010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38800_length_3067_cov_6.457835"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            764..1630
                     /locus_tag="PL056_12825"
     CDS             764..1630
                     /locus_tag="PL056_12825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPM47947.1"
                     /translation="MKSAIDPQELAEIKNLISASVQSIPGRVPEIQVVPEDQADTVIE
                     LITTVKKEKSWKTRKTNDPAFGMCYSQGEDNPRVDLTIDMIMNVNKKGETVQQVTRKA
                     GSEGTLPYNTDCYFSLKPGETREPEIISECQTRTKRAGQEGYLKASVNFDEWFATIKN
                     IAFKQIPSAIVSPFLILEENVPVIFYKIKKPEGVKEANGHLSNGNWSEAKEIYENLIS
                     SLSSTPDLNPEDEAKFYHNYAITLMAEGDFEKAMEWLVKAKGLVDTDRHDRAIETLKY
                     LIENEKKLNEQK"
     gene            1932..>3067
                     /locus_tag="PL056_12830"
     CDS             1932..>3067
                     /locus_tag="PL056_12830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47948.1"
                     /translation="MKKIAFILFAFFSTIYAVDDVSKTVADESQKKYERKAILTVDTV
                     GFGSPSFNAPVTFCPYQAKQCAQRGFVSYSVTNTARANASGNFFDLLVTKRFDRIFVG
                     TDSAVIITPKSSTKIDFPGGTFPEFSFSSGTAEDFKMRVDSLLNNKLSGMMDAVLSSA
                     VEKRYKELPETEQGMFIQEKAKELGISSLFIEKLMRSGFVFSVFIPDMSGSVSVSENR
                     ITDSKGNTRTTYSATASIVAFPKIIIYRYDPEKRAFTEYNTISLRAAGSSGRSFNSYP
                     GSDNFTGMFDTAVAEFLNKAGEKGNFELKKDDNFAIFSGVEEVDWFKVKSSIGTNEDL
                     RIDAPYYVERKIDDKMKKIGIVKAKKVFDNTENQGQSHFRRIRGS"
BASE COUNT         1063 a          465 c          652 g          887 t
ORIGIN      
        1 ttggaatccc gccggcgata gttgtcgcca aatcacttgg aacatatctc aatccggtta
       61 ataagaagtg tgtcagctcc gccgtatcag acgaacttga gaaaagaaaa gctcagaagg
      121 atgtggataa gtatctttaa ttaatttcaa cacaagtgct ttaatcagaa aaagccgtaa
      181 atgtagtcta tgtccaacag aaaatttgac atataattaa acagcttgta ggatatagac
      241 aacaaatgtt gctttgtaaa tgattatggt tgtttttaat gaatgacagc atgattcctg
      301 ttgttaaaat tacaaaaaag agtttttcct ccgatgcaaa tattggtttt gacggcgttt
      361 tagatctgaa tgcaggagca ggtttaaaac gctgtcggga caatttttta atgattacag
      421 gtgatgttta taaatgcata aatcgtttcc attttaattt ttgaaggagg attccatgaa
      481 acagagaatg ttttttatcg tgttggttgt tttattagca atttcatgtt caacgggaac
      541 agcaaacttg agaattcaga aggcggcgga aattaaccac aagaaattgg gcacagttgc
      601 ttttgttgtc aatgatccga ctggcgaaga aactttcacg agattaaaag aatgtattcc
      661 tgaacctgtt gaggaaactg tagctgaagg tggagtctca aatgaagatg gatctgcttc
      721 agccggattt tcactgtcta tttcaagtaa taaaccgaga attatgaaat ctgctattga
      781 tccgcaagaa cttgctgaaa taaaaaatct aataagtgcg tcagtacaat caataccagg
      841 aagagttcca gaaattcagg ttgttccaga agatcaagca gatacagtaa tagaattgat
      901 aacaactgtt aaaaaggaaa aatcatggaa aacacggaaa acgaacgatc ctgcttttgg
      961 aatgtgttat tctcaaggtg aagacaatcc aagagttgat cttacaattg atatgataat
     1021 gaatgttaac aaaaagggtg aaacggtaca gcaagttaca agaaaagcgg gttccgaagg
     1081 cactcttcct tataatacag attgttattt cagcttaaaa ccaggggaaa caagagaacc
     1141 tgaaataata agtgagtgtc aaacgagaac gaagagagct gggcaggaag gatacttaaa
     1201 agcaagtgta aattttgatg agtggtttgc aacaataaaa aatattgctt tcaaacaaat
     1261 tccttcagca atagtaagtc cttttttaat tctcgaagaa aatgtgccag tgatttttta
     1321 taagattaaa aaacccgaag gagttaaaga agcgaatggt cacttgtcaa atggtaattg
     1381 gtctgaagca aaggaaatat atgaaaacct tatttcatct ttaagctcaa ctccagatct
     1441 taaccctgag gatgaagcaa aattctatca caattatgct ataactctta tggcagaagg
     1501 agattttgaa aaagcaatgg aatggcttgt taaagcaaaa ggactggtgg atacagatag
     1561 acatgacaga gcaatagaaa cattaaagta tctcattgaa aatgaaaaga aacttaacga
     1621 acaaaaatag tgatggagaa aactatgaaa aaaatgtttt tgttgatttg cgtttcagtt
     1681 gctttcaatt ttttgttaat atcctgtgac ccaggaaaag attgtggaga aagttgcaag
     1741 gaagatgatg attgcaacat atcaagacta atttgctctc ctggtggtta ttgtgttcca
     1801 acagagtgtc gagattgcta taacaatggt gaggcttgtt acacgactga acctgagaac
     1861 ggaacatgtg attttaaata ttgcggctat taaatttccc caaaacattc aataaaaagt
     1921 acgaggatta aatgaaaaaa attgcgttca tattatttgc atttttctca actatttacg
     1981 cagttgatga tgtttcaaag acggttgctg acgagtcaca gaagaagtat gaaaggaaag
     2041 ctattcttac tgttgacact gttggtttcg gaagtccaag ttttaacgct cctgtaactt
     2101 tctgtcctta tcaggcaaag cagtgtgctc agaggggatt tgtttcatat tcggtgacaa
     2161 acacggccag agcaaatgct tcaggcaatt tttttgatct tcttgtcaca aaaagattcg
     2221 accgaatttt tgtggggact gacagtgctg tgataataac tccgaaatct tcaacaaaaa
     2281 ttgattttcc agggggaact tttcctgaat tttctttttc cagcggtaca gctgaagatt
     2341 ttaagatgag agttgattct ctgctgaata ataaactttc agggatgatg gatgctgttc
     2401 tttcctcagc tgttgagaaa agatataagg agcttccgga aactgaacag gggatgttca
     2461 ttcaggaaaa agcaaaggaa cttgggatat catcattatt tattgaaaag ctgatgcgtt
     2521 caggctttgt tttctctgtt tttattccgg atatgtcagg cagtgtaagt gtcagtgaaa
     2581 acaggataac tgacagcaag ggaaatacaa gaacaactta ttcagcaact gcttcaatag
     2641 tagcatttcc taagatcatc atatatcgct atgatcctga aaaaagggca tttactgaat
     2701 acaacaccat ctcccttaga gcggcaggaa gcagcggaag atcgtttaac agttatccgg
     2761 gatcagacaa ttttaccggc atgtttgata cagcagtggc agagttttta aataaggctg
     2821 gagaaaaagg gaattttgag cttaaaaaag atgataattt tgcgattttc tcaggagttg
     2881 aagaggttga ctggtttaaa gtgaaatcat caatcggaac aaatgaagat ctccggatag
     2941 atgctcctta ttatgttgaa aggaagattg atgataaaat gaaaaagatc ggtattgtca
     3001 aagcaaaaaa agtttttgac aacaccgaaa atcagggaca aagtcatttc agaagaataa
     3061 gaggttc
//
LOCUS       DAOOYB010000323         3048 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_39168_length_3048_cov_12.267624, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000323 DAOOYB010000000
VERSION     DAOOYB010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39168_length_3048_cov_12.267624"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="PL056_12835"
     CDS             complement(<1..793)
                     /locus_tag="PL056_12835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47949.1"
                     /translation="MKSFFKLFSFFLAVLFVLSSCDSSTIKTKKNDDNNSDSDEDIEN
                     INDSDDIGDTGNTGDSGDTGNSGNSGNTGDTVSETCGDNIVDTGEICDGNVINCVDID
                     SQLYSGGKSKCLDTCLGWDTATCDEIPHTCGNDITEGPEECDGNIIDCNELDSEKYES
                     GKAKCLDDCSGYDTLTCVEYEPSTCGDEKVEGKEVCDGGLKNCVEIDDGKYSGGKAYC
                     KNDCTGWDEITCDEKPVCSAPYRTVKCAGDDALDQMQTCSEGIWSN"
     gene            complement(899..2614)
                     /gene="argS"
                     /locus_tag="PL056_12840"
     CDS             complement(899..2614)
                     /gene="argS"
                     /locus_tag="PL056_12840"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005903653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HPM47950.1"
                     /translation="MELVTIEISRILKEAVLKINPDFTDWDKLFLQPASSEKFGDYQT
                     NFAMAASKNFGKPPRVIAENIVSALDKNNVIEKLEVAGPGFINIFINRTYLAEIVKLS
                     PGIKWNFSHVDTAGDVVIDYSSPNIAKPMHAGHLRTTIIGDSIKRIMRYIGYNVIADN
                     HLGDWGTQFGKLIVAYKRWLDEENFKKDPISELERIYIKFGKEAEDDTTLDDLARAEL
                     KKVQDGDSGNLDLWKQFVEITLNECKRIYKYLDIDFDTYYGESFYHNMMPGVVEELVK
                     KEIAVESDGALVVFFDETENLHPFIIRKKDGAYLYSTSDLATAKFKYENYSVNKAVYV
                     TDDRQETHFKQVFRTAQRIGWNMEFVHVTFGIMSFNGVILSTREGNTVKLNDLFAEAE
                     AKAFAIVTEKNPDIPEDERKKIAKAVGIGALKYSDLSQNRTSAIDFRWDKALSFEGNT
                     APYLQYSYARIQSIKRKAAETGIKLDHSAKIVLETEIEKQIGSSIVRFPECVQKAAEY
                     YKPNFIADHIYDLAQKFSSFYNSTPVLKAENELVQSRLLLSESVAHTIKTGLNLLGIE
                     VVERM"
     gene            2751..>3048
                     /locus_tag="PL056_12845"
     CDS             2751..>3048
                     /locus_tag="PL056_12845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47951.1"
                     /translation="MKKVHSILMLLTVFVFLSACVETQVEVKPVDNDIINSEGPDVVP
                     DETETVVDEEQKNDDEAVSPDDEDPGESDCATANLGKSCTTDTDCGKCMICITNG"
BASE COUNT          808 a          644 c          600 g          996 t
ORIGIN      
        1 tgtttgacca gataccttca gaacaagtct gcatctggtc aagagcatcg tccccggcac
       61 attttactgt tctgtatgga gcactgcata ccggtttttc atcgcatgtg atctcatccc
      121 agccggtgca gtcatttttg cagtaagctt tcccgcctga gtattttcca tcatcgatct
      181 caacacagtt tttcaaccca ccgtcacaaa cttcttttcc ttcaactttt tcgtctccgc
      241 aggtgcttgg ttcatattca acgcaggtca aagtgtcata gccggagcag tcatcaagac
      301 attttgcttt tccgctttcg tatttttcgc tgtcaagttc attgcagtca atgatattcc
      361 cgtcacactc ttccggacct tcggttatgt cgtttccgca ggtatggggg atctcgtcac
      421 atgttgccgt gtcccatccc agacatgtgt caaggcattt tgattttcct ccggaataga
      481 gttgtgaatc aatgtcaacg cagtttatta cattgccgtc acatatttcg cctgtatcga
      541 caatgttgtc tccgcaggtt tcagaaactg tgtcaccggt attgccggaa ttgcctgaat
      601 ttccagtgtc gcctgaatct ccggtgttgc ctgtgtcgcc tatgtcatcc gaatcattga
      661 tgttctcaat atcttcatca gaatcagaat tgttgtcatc attctttttt gtttttatcg
      721 tactgctgtc acatgacgac agaacgaaaa gaactgccag aaaaaaagag aacaatttaa
      781 aaaaactttt cattttaccc tccccggaaa ttatctaaaa tcacaaaatt atacatgaaa
      841 caggttgccg agcaagaaaa gaaatttatt ctctgtgttt aaggagatat ttttgtaatc
      901 acatcctttc tacaacttct atcccgagaa gattcaggcc tgtttttatg gtgtgtgcga
      961 cactttctga taaaagaagc cgtgactgga caagttcgtt ctcagctttg agaacggggg
     1021 ttgagttata gaaactgctg aatttctgtg caaggtcata aatatggtcg gcaatgaagt
     1081 tgggtttata gtattctgcc gctttttgaa cacattccgg aaagcggaca attgatgagc
     1141 caatctgttt ttcgatctcg gtttccagaa cgatctttgc tgagtgatct aattttattc
     1201 ccgtttcagc cgcctttctt tttattgact gtattcttgc atatgaatat tgaaggtaag
     1261 gtgctgtatt tccttcaaaa ctcagggctt tgtcccatct gaaatctatt gcgctcgttc
     1321 tgttttgaga caggtcggaa tatttgagtg caccgattcc gactgctttt gcgatctttt
     1381 ttctctcgtc ttcaggaata tcgggatttt tttcggttac tattgcgaaa gcttttgctt
     1441 cagcttcagc aaaaagatcg ttgagcttta cagtatttcc ttcgcgggta ctcaggatca
     1501 caccgttaaa agacatgatt ccgaatgtca catggacaaa ttccatgttc cagccgatcc
     1561 gctgtgcagt acggaaaacc tgtttaaaat gggtctcctg cctgtcatca gtcacataaa
     1621 cggctttatt tactgaataa ttctcgtatt tgaattttgc tgtcgcaagg tcgcttgtgg
     1681 agtaaagata tgccccgtcc tttttcctta ttataaaagg atgaagattt tccgtttcat
     1741 caaaaaagac cacaagagcc ccgtcacttt cgaccgcgat ctcttttttg acaagttctt
     1801 caactactcc aggcatcata ttgtgataaa aggattctcc gtaataagtg tcaaaatcaa
     1861 tatcaagata tttgtatatc ctcttgcatt cattgagagt tatctctacg aactgtttcc
     1921 acagatcaag gtttccgctg tctccgtcct gtactttttt aagctcagcc cttgccagat
     1981 catctaatgt tgtatcatct tcggcttctt tgccgaattt tatgtaaatc ctttcaagtt
     2041 ctgatatcgg atctttttta aagttctctt cgtcaagcca ccttttgtaa gcgacgataa
     2101 gcttgccaaa ctgtgtcccc caatcaccga gatgattgtc ggcaatgaca ttataaccga
     2161 tatatcgcat tatccttttt atgctgtcgc cgattatggt tgttctcaaa tgtcctgcat
     2221 gcatcggttt tgcgatattg ggactggaat aatcaattac aacatcaccg gctgtatcaa
     2281 catgagagaa attccactta atgcctgggg aaagcttaac aatttcagca agatatgtcc
     2341 tgttgatgaa aatgtttata aatcccggcc ctgcaacttc aagtttttct atcacattat
     2401 ttttatcaag tgccgacact atgttttcgg caatgactct gggtggtttt ccaaaattct
     2461 ttgaagccgc catcgcgaaa tttgtttgat aatcgccgaa cttttcactg cttgcgggtt
     2521 gcagaaacag tttgtcccag tcagtgaaat cagggtttat cttcaaaact gcttctttta
     2581 aaatccttga aatctcaata gttacaagtt ccatgttccc tcagaaaaat cagataatta
     2641 caaaatgata tatcataaaa gagaaaagaa aacaactttg gagtacagca attccgttat
     2701 aaataatggg tgtttgaaaa ggttattaat cttgttgagg gaggccggtc atgaaaaaag
     2761 ttcacagtat attgatgtta ttgacagttt ttgtgttttt gtcagcttgc gttgaaactc
     2821 aggtggaagt taagccggtt gacaatgaca ttattaattc tgaaggaccg gatgtcgttc
     2881 ctgatgaaac agaaacagta gttgatgagg aacagaaaaa tgatgatgaa gcggtatctc
     2941 cagatgatga agaccccggg gaaagcgatt gcgcaactgc gaatctgggc aagagctgta
     3001 caactgatac agactgcgga aaatgtatga tctgcataac aaatggaa
//
LOCUS       DAOOYB010000324         3047 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_39184_length_3047_cov_7.089238, whole genome shotgun sequence.
ACCESSION   DAOOYB010000324 DAOOYB010000000
VERSION     DAOOYB010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3047
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39184_length_3047_cov_7.089238"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(180..>3047)
                     /locus_tag="PL056_12850"
     CDS             complement(180..>3047)
                     /locus_tag="PL056_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013914208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HPM47952.1"
                     /translation="MPNFWPYFDAATYNSIPDGQMVRYTYDGQVINLKNSPPASYDYY
                     LPNGNPMPHDDLVSYYSHHAKPGAYCDWPAETADYNKNVMNHMQSQTHVTMSASVINN
                     VQSFVELQNHDQFNSRVNWGEKWRNSHFNTRTASGFPALDLIHFSGHHSMGPLVGNEY
                     LLKELIYHNVTLSRDYFLGSNFKSSKGFFPTELGFSTRIIPVLKKIGVEWSVIANVHF
                     SRTLTDYPYLNDPGIDCMISPPNRADMRNVDSYGGAWVSRQMFNEQQVTQNKFPFASI
                     PHFVRYIDPWTGAEMKVAGIPVEQAASWEEGYQGSKAPVDVTWLHTYDQYANGRIQYH
                     VIAHDGDNSQGAAGDGGTWLVSGNSIYTQTGVTGKGVEEYLKDHPIPENDIVHVQDGS
                     WIDTRDSSSDPTWYHWHLPFGIWKGQFADFNAKTGKNFAPKKNFQGTDEGMTVSMEYG
                     YHYLERNFALLQPALNYAVTAEQIWLDKNPGHWKPESPLDLEITGKSNTENQLNPYLM
                     SYPVKGSSNAAELAWYFLIAAIDSGFGYYDENVDDGVKPTISFNQSLYFSVPYVTQNI
                     AEDRTGPSIWWPQRWPYNPGSANKSKAEGWTLHYFDNNFVIYTYGYDVSGIKNIRVRI
                     RKHKNKVADPLDRTYQVYNPQLLKDKGVPQINPGNVGEWISYPMERRDLTSDINGVEW
                     QPSGQEIMEIVPAEKIGNLYFSYIGDYRDELIDYYIEAVDTKDNVSRSEIQQVYVGTG
                     KFKNEGGKIVEDINGDMDGTYPFLEKKTTDPADDDSDEFDVDEDFQYDSELPDNDDLS
                     DEVLTESEELPDDENPVPDGFFRAHYFHENWETVNIHYCLENCEDVPNWTEVPGAAME
                     DESDKWFKYEGGYWDGYVEFVFNDNSGTWDNNSENNYKTELQEFWVKNGEIYPSNPDK
                     PEKNDGDDPENEDDDDFSYTDFTDDDNIDDKRSSGCGCSLI"
BASE COUNT          778 a          729 c          616 g          924 t
ORIGIN      
        1 gtgctgatta ttccgccgtg aacgatccct tcccatccgc agaatttcct gtctattttt
       61 gtaacggccc acgctttttt taaggacttg tcactttcga tcttaagttt aagaccggta
      121 ctgttttcac aaccgcatac aaaacatgat ctgtcgtcat taagttccat ttttccctct
      181 catatcagtg aacagccgca acccgaagat cttttatcat caatattatc atcatctgta
      241 aagtctgtat aagaaaagtc gtcatcgtct tcattttcag gatcatcacc gtcatttttc
      301 tcaggtttgt cagggtttga cggataaatt tcaccgttct ttacccagaa ttcctgcaac
      361 tctgtcttgt agttattttc gctgttgtta tcccatgtcc ctgagttatc attaaaaaca
      421 aattcgacat atccatccca gtatccacct tcatatttaa accatttgtc actttcatct
      481 tccattgcag cacccggaac ttcggtccag tttggaacat cttcacaatt ttcaaggcag
      541 taatgaatat tgaccgtttc ccagttttca tggaaataat gagccctgaa aaagccgtca
      601 ggaacagggt tttcgtcatc aggcagttct tccgactccg ttaaaacttc gtcggacaag
      661 tcgtcattat caggcagctc ggaatcatat tgaaaatcct cgtcaacatc aaattcatcg
      721 ctgtcatcat cagccggatc tgtggtcttt ttttcaagga atggataagt cccgtccatg
      781 tcaccgttga tgtcttcaac gatctttcca ccttcatttt taaatttccc tgttccgaca
      841 tagacctgct gaatttcact tcttgaaaca ttgtcttttg tgtcaactgc ttcaatgtaa
      901 taatcaatca gttcatcgcg gtagtcaccg atgtatgaaa aatagagatt cccgatcttt
      961 tcagcgggaa ctatctccat aatttcctgt cctgacggct gccactcgac accgttgatg
     1021 tcggaggtaa gatcccgccg ctccatcgga taactgatcc attcacccac attgccgggg
     1081 tttatctgcg gcacaccttt atctttcaga agctgaggat tataaacctg ataagttctg
     1141 tccagcggat cggcgacttt atttttgtgt tttcttatcc tgacacgaat gttcttgatc
     1201 cccgaaacat catagccata ggtatagatc acgaaattat tgtcaaaata gtgcagagtc
     1261 cagccctccg ccttgctttt atttgcactt cccgggttgt aaggccaccg ctgaggccac
     1321 cagatcgaag gtcccgttct atcttcagca atgttctgcg ttacataggg aactgaaaaa
     1381 taaagtgact ggttaaatga aattgtgggc ttaactccgt catcaacatt ttcgtcatag
     1441 tatccgaaac cgctgtcaat tgccgcgatc agaaagtacc atgcgagttc tgccgcattg
     1501 cttgagccct tgaccggata gctcataaga tatgggttaa gctggttttc agtgttgctt
     1561 ttcccggtta tttcaagatc aagtggtgat tcaggtttcc agtgacctgg gttcttgtca
     1621 agccagatct gttcagcggt aacggcatag ttaagtgccg gctgaagaag tgcgaagttc
     1681 cgttccagat agtgataacc gtattccata gaaactgtca tcccttcatc agttccctga
     1741 aaattctttt tcggagcgaa attttttccc gttttagcgt tgaaatcggc aaactgccct
     1801 ttccagatac caaacggaag atgccagtga taccatgtcg gatcgcttga tgaatcgcgg
     1861 gtatcaatcc agcttccatc ctgaacatgg acgatgtcat tttcagggat aggatgatct
     1921 ttcaaatact cttcaacccc ttttccggta acaccggtct gggtatagat cgaatttccc
     1981 gaaacaagcc atgttccgcc atctcctgcc gcaccctgcg aattatcgcc gtcgtgggca
     2041 atgacatgat attgaattct tccatttgcg tactgatcat aagtgtgaag ccatgtcaca
     2101 tcaacgggtg ctttacttcc ctgatatcct tcctcccatg aagcggcctg ctcaacagga
     2161 attcctgcaa ctttcatttc tgcaccggtc catggatcga tgtatcgcac aaaatgggga
     2221 attgaagcaa acgggaactt gttctgagtc acctgctgtt cattgaacat ctgcctggaa
     2281 acccatgccc cgccgtaact gtcgacattc ctcatgtcag cccggttcgg aggagagatc
     2341 atgcagtcga ttcccggatc gttgagataa ggataatcgg tcaaagtcct tgaaaaatga
     2401 acatttgcaa tgacactcca ctcaacgccg atctttttta aaaccggaat tatccttgtt
     2461 gaaaagccga gttcagtcgg aaaaaaaccc tttgaagatt taaaattgct ccccagaaaa
     2521 taatctcttg aaagtgttac attatgatag atcagttcct ttaaaagata ttcgttcccc
     2581 acaagcggac ccattgagtg gtggcctgaa aaatgtatca gatcaagagc gggaaatccg
     2641 ctcgcagtcc ttgtgttaaa atgggaattg cgccattttt ctccccagtt tactctgctg
     2701 ttgaactgat cgtgattttg aagttcaacg aagctctgaa cattgtttat gacagaagcg
     2761 ctcattgtga catgtgtctg actctgcatg tgattcatca catttttatt gtaatcggcg
     2821 gtttcagccg gccagtcgca gtaagcaccg ggcttggcgt gatgggaata atatgagaca
     2881 agatcatcgt gaggcatcgg attgccgttt ggaagataat agtcatagct tgcaggcgga
     2941 ctgtttttta agtttatcac ctgaccgtcg taggtatatc tcaccatttg accgtcaggg
     3001 atcgagttat atgtcgcagc atcaaaataa ggccagaagt tcggcat
//
LOCUS       DAOOYB010000325         3041 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_39293_length_3041_cov_10.240455, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000325 DAOOYB010000000
VERSION     DAOOYB010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39293_length_3041_cov_10.240455"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="PL056_12855"
     CDS             complement(<1..530)
                     /locus_tag="PL056_12855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47953.1"
                     /translation="MRKNLFVLTVFVSVLIFGCDDGSAKNDADIISIADEDLSDDLSD
                     ENLTDSEENDSDTAEITDDPAEIINIGLDESFLEPNKGFAFKIVAPLLPAVPSSYDLK
                     PEEKPHYLQGELTDKSGNKFVFSNIDNTFAADYEGYLYITSASEYSETTYYTANILPS
                     FETLDWMTENEVDTVNS"
     gene            742..1800
                     /locus_tag="PL056_12860"
     CDS             742..1800
                     /locus_tag="PL056_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HPM47954.1"
                     /translation="MKQKKNETPQRKAKKEISIIRSSAAEYLTFVAAGGRGGVEAVYS
                     DENIWLTQKMMGVLYDVDVRTINYHLKKIFSDSELQEDSVIRNFRITATDGKNYDTNH
                     YNLSAIIAVGYKVNSERAVQFRKWATTIIKEYTIKAYVMDDERIKKGGSILTEQYFEE
                     QLQRIREIRMSERKFYQKITDIYSTSIDYDVTALSTKRFFATVQNKLHWAIHGATAAE
                     IIYNRADAKKENMGLNTWKDAPKGKIQKFDVSVAKNYLTEEELAQLERLVSAYLDVAE
                     SMAIRKIPMTMADWETRLNRFIEATDREILQDAGKVTAEIAKAHAESEFEKYRIVQDR
                     LFESDFDRMIKKLEHKKE"
     gene            complement(2081..>3041)
                     /locus_tag="PL056_12865"
     CDS             complement(2081..>3041)
                     /locus_tag="PL056_12865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPM47955.1"
                     /translation="KKIIPAIISDFRSCGGIVFFETEVTEIHRSGDKITALEITGKQN
                     GKLEGDVFIFAPGHSSFNLYRMLLENNVQFSTKPFAIGFRVEHLQEAINLAQWGTASL
                     PGVKAAEYRLAESFDNSSVFSFCMCPGGKVVQSSPESGLSVVNGMSNYSRNGIFANSA
                     VVTPFKIEEISNAHVSPLTALDLLEDLEKKFYNSTNSFDIPATNITDLINGKTSNRFS
                     SSSYSFNLVSRDLHELFPKTVLERLINGLKIFNNKIRGFQDGVAMGLESKTSSPLKAQ
                     RNQNLTTQPFSNLYICGEGSGNAGGIISSAADGVRVAMAVIRI"
BASE COUNT          987 a          556 c          639 g          859 t
ORIGIN      
        1 gaattaactg tatcaacttc attttcagtc atccagtcaa gtgtttcaaa tgacgggaga
       61 atgtttgcgg tataatatgt ggtttctgaa tattctgaag cagaagttat ataaagataa
      121 ccctcataat ctgccgcaaa agtgttgtca atgtttgaaa acacgaattt atttcccgac
      181 ttgtctgtta attcaccttg aagataatga ggtttctctt caggtttaag atcatagctt
      241 gaaggtacag caggaagtag cggtgcgact attttgaaag caaacccttt gttcggctca
      301 agaaatgatt catcaagccc aatgtttata atctcagccg gatcatctgt gatttcagcc
      361 gtatctgagt cattttcttc tgaatccgtt aagttttcat cggataagtc atcagacaaa
      421 tcttcatccg caattgaaat aatgtcagca tcattttttg ctgaaccatc atcacaacca
      481 aaaattaaaa ctgaaacaaa aacagttaga acaaatagat tttttctcat ggcgaacctc
      541 caaaaaacaa attcaggaca atatctgcaa aaatagtacc taagaaaaaa gttcaatgaa
      601 atcaagaggt atgtcaggtt tttgaagcac acaaattcgt ttcagatatt gtgaggtctg
      661 tttgaagatg cctgttgact ttaattttac cttctattat tctgttttga ctgtttagta
      721 tgaaaaagtt tgaggattaa tatgaaacag aagaaaaatg aaacgcctca gagaaaagcg
      781 aaaaaagaaa tatcaattat tcgctcatcg gctgccgaat atctgacatt cgtagcggca
      841 ggaggcaggg gaggtgttga agcagtttac tccgatgaaa acatctggtt gacccagaaa
      901 atgatgggag tgctttacga tgttgatgtc aggacgatta actatcactt gaaaaagata
      961 ttcagcgaca gcgagttaca ggaagattca gttatccgaa attttcggat aactgccact
     1021 gacggcaaaa actacgacac caatcactat aatctatcgg caataattgc agttggttac
     1081 aaggtcaact ctgaacgggc tgtgcagttt agaaaatggg cgaccaccat tattaaggaa
     1141 tataccatca aagcatatgt aatggatgat gaacggataa aaaaaggcgg ttctatcctg
     1201 actgagcaat actttgaaga gcagttacag cgtattcggg aaatacgcat gagtgaacgc
     1261 aaattctatc agaaaatcac cgatatatat tcgacttcta ttgattatga tgttaccgct
     1321 ctatccacta aacggttttt tgccactgtt cagaacaaac tgcactgggc tatacatggt
     1381 gctactgcgg cggaaattat ttataaccga gcagatgcaa aaaaagagaa catgggatta
     1441 aatacatgga aagatgctcc gaaaggaaaa attcagaaat ttgatgtaag cgttgccaaa
     1501 aactacttga ctgaggaaga gctggcacaa ttggaaaggt tggtgtcagc atatcttgat
     1561 gttgcagaaa gcatggcaat tcgcaaaata ccaatgacta tggcagattg ggaaacccgt
     1621 ctgaatagat tcattgaagc tacagacagg gagatcctcc aggatgcagg aaaagtaacg
     1681 gcggaaattg ccaaagctca tgctgagagc gaatttgaaa aataccggat tgttcaggat
     1741 cgcctttttg aaagtgattt tgacaggatg ataaaaaagt tagagcacaa gaaagaataa
     1801 agtaagggcg aaaaatcttt cgcccctacg gcaacatatt gaaaaagttt gaaattaaga
     1861 tattattcac agggctgaga attgccgctg cgaatgcccc tgtgaaagca ggagaattaa
     1921 tccgggtgga tgcggctacc ggacagcccg tttttgtttg ggaagatctg atttgaagat
     1981 aaatcttaga aaaacgactg aactttcatt aatattgcaa aatatcgaag aaaacacaga
     2041 tgttcttata aaagtggcaa catattgaaa aaacctgcac ttaaattcta atcacagcca
     2101 ttgcaactct tactccgtca gcggcacttg agattatgcc gcctgcattt ccgcttcctt
     2161 ctccacagat atagagattg gagaagggct gggtggtaag gttttgattt ctttgagctt
     2221 tcaagggcga agaggttttg ctttcaagtc ccattgcaac accatcctga aatcctcgaa
     2281 tcttattatt gaatattttg agcccgttta tcagccgttc aagaactgtt ttcgggaaaa
     2341 gttcgtgaag atctctgctt acaaggttga atgaatagct tgatgatgaa aaccggtttg
     2401 aagttttccc gtttatgagg tcagtgatgt ttgttgcagg aatatcaaat gaatttgtag
     2461 agttataaaa ctttttctca agatcttcaa gaagatcaag tgcagtcagc ggtgaaacat
     2521 gagcattgga aatctcttca attttaaacg gggtgacgac agcactgttg gcaaaaattc
     2581 cgtttcttga ataattgctc attccgttca cgaccgaaag tccgctttcg ggagatgatt
     2641 gtacgacttt tccaccgggg cacatgcaga acgaaaatac agaactgttg tcgaaacttt
     2701 ctgcaagccg gtattcagcc gctttaactc ccggaagtga agccgttccc cactgagcaa
     2761 gatttatggc ttcctgaaga tgctcaaccc tgaagccgat agcaaaaggc ttggttgaaa
     2821 attgaacatt gttttcaagc aacatcctgt aaagattgaa agaactgtgg cccggagcaa
     2881 aaatgaaaac atctccttcg agctttccgt tttgctttcc ggttatttca agagcggtga
     2941 ttttatcacc tgatctatga atttcagtca cttcagtttc aaaaaaaaca attcctccgc
     3001 atgatctgaa atctgaaatt attgcaggga taattttttt t
//
LOCUS       DAOOYB010000326         3033 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_39438_length_3033_cov_9.733378, whole genome shotgun sequence.
ACCESSION   DAOOYB010000326 DAOOYB010000000
VERSION     DAOOYB010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39438_length_3033_cov_9.733378"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="PL056_12870"
     CDS             complement(<1..257)
                     /locus_tag="PL056_12870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47956.1"
                     /translation="MARVVITFISALFIMFSFLTCSKSDDKDCINTKDCPKGYECIDF
                     KCKEETAGDTGDTGNTGNTGNSGDTGDTGNSGDTGNTGNTG"
     gene            complement(279..1136)
                     /locus_tag="PL056_12875"
     CDS             complement(279..1136)
                     /locus_tag="PL056_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013941899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPM47957.1"
                     /translation="MPGKKNVGILRVLLMHLSLMLVCAAVIYPVLWVIKMALSPEQGF
                     SLSANPFPVEFSLDNFRHVIFTTDSSGGWLFGRQFFNSVVISAATTFLGITLATTAGY
                     AFSRFEFPGKDLGMKMFLVSQMFPGVVMAIPLYILLDNLGLLNSMTGLVLVYSTTAIP
                     FCVWMLKGYFDTIPKDLEEAAIIDGASQWTIFFTVIMPLAKPAIVVTALFSFMTAWNE
                     FILAATFLGNEKSFTLPVTLQHYVGSYSTEWGYFAAGAIIVSIPVMVLFFILQKSLIG
                     GLTAGGVKG"
     gene            1342..1417
                     /locus_tag="PL056_12880"
     tRNA            1342..1417
                     /locus_tag="PL056_12880"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1375..1377,aa:Lys,seq:ttt)
     gene            1435..1509
                     /locus_tag="PL056_12885"
     tRNA            1435..1509
                     /locus_tag="PL056_12885"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1468..1470,aa:Glu,seq:ttc)
     gene            1604..2794
                     /locus_tag="PL056_12890"
     CDS             1604..2794
                     /locus_tag="PL056_12890"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPM47958.1"
                     /translation="METATPFIFAGFSGAAVICAILLFFAKESYPALKMLGILLLIFA
                     IDCAFAVFMISKGYRTFPHLIGLSRPLIFMYGPLFFLIFSIATGYHSRVKTDHLFHFI
                     PFLTHFSLMTPFIFKTPATKIFLYEHYVKGVAYIEYYPHAMMFLTVIFFLMYSVLPLA
                     LTYKYKKTVMGFYSTIDTRKILKLYLFSALYIICAIYLTFVFHGYLTGPSFIRPDIPF
                     HFLYFLAAIIFPFTFMAITDLSFLRPSLSIHDLIFEDEPAEKTDSEEKTEVSTAEKYQ
                     KNKMPDELAQKAFELLKEVMAEKNPFTDSNLTLVDLAEMIHIRPYHLSQLINSRTGDN
                     FYVFINSARIKYAAELLKDPVHKEKSILEIIYMSGFNSKSAFNTYFKNIMGETPSNFR
                     KKHS"
     gene            complement(2842..>3033)
                     /locus_tag="PL056_12895"
     CDS             complement(2842..>3033)
                     /locus_tag="PL056_12895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47959.1"
                     /translation="TSAISAFSGVEHEKAKTVKMFKIAIIFLMTLSIRSVDKKPGYIF
                     RLNDFFCLFVLSHEEEQIA"
BASE COUNT          895 a          690 c          537 g          911 t
ORIGIN      
        1 tcaccagtgt ttccagtgtt tccggtatcg ccactgtttc cggtatcgcc tgtatctccg
       61 gaattgcctg tgttgcctgt atttccagtg tcgcctgtgt cgcctgctgt ctcctctttg
      121 cacttaaaat cgatacactc ataaccttta ggacaatcct tggtatttat gcagtccttg
      181 tcgtctgact tgctgcatgt aaggaacgag aacataataa acagtgcaga aatgaaagtg
      241 ataactactc ttgccatttt atcctcctga aaaaactgtc aaccctttac gccgcctgca
      301 gtaagccccc ctatcagact tttctgcaga atgaaaaaaa gtaccattac aggaattgaa
      361 acaatgatgg ctcctgcggc aaaatacccc cattcagtcg aataacttcc gacataatgc
      421 tgtaaagtta caggaagcgt gaacgacttc tcgttaccta gaaatgttgc ggcaagtatg
      481 aattcgttcc atgctgtcat aaagctgaaa agtgccgtca cgactattgc aggctttgca
      541 agcggcatta tcactgtgaa aaatatagtc cactgcgacg ctccgtcaat gatcgccgct
      601 tcctcaagat cttttggaat agtatcgaaa tatcctttga gcatccagac acagaaaggt
      661 atcgccgttg tagaataaac aagaacaagc cctgtcatac tgttcagaag cccgagatta
      721 tcaagcagaa tgtaaagcgg tatcgccata acaacaccgg ggaacatctg gcttacgagg
      781 aacatcttca ttccaaggtc cttccccgga aattcaaacc ttgaaaaagc ataacctgct
      841 gtagttgcta aagttattcc taaaaatgta gtggcggctg aaataacaac actgttaaaa
      901 aactgccttc cgaaaagcca gccacccgaa gaatcggttg taaaaatgac atgtctgaag
      961 ttatcaagtg aaaactcaac aggaaacgga tttgctgaaa gagaaaatcc ctgctcagga
     1021 ctcagtgcca tttttatgac ccagagaaca ggataaatga cagcggcaca gacaagcatt
     1081 aaggagagat gcatcagcaa aacccttaaa attcctacat ttttcttccc aggcattgta
     1141 aatcctccaa aaatcccatg attttaactt atatttcata atttttcaaa caaactctct
     1201 tctcacagca aacaatgtta ataaatgaat aattgtgtgc acttacattc ttttcaaaca
     1261 aaaagtttga caaagaagac ctttacacta ctatacacgg gctactttgg tcgttatcaa
     1321 tcgattataa agtgttgtgt tgggtcggta gctcagctgg tagagcatct gacttttaat
     1381 caggtggtcg tgggttcgaa ccccgcccga cccaccattc tcattttttc aggcgaccct
     1441 ttcgtctagt ggttaggaca tcggcctttc acgtcggtaa cgggggttcg agccccccaa
     1501 gggtcgccac ttttttttct aagagatgtc gttaattcat tcaatttaca tctgatctgt
     1561 tttctgttat attgatatga acttgtcttt gcagggaatg atcatggaaa cagcaactcc
     1621 ttttattttt gccggatttt caggagcggc ggtgatttgt gcgattcttc ttttttttgc
     1681 aaaagagtct tatccggcat tgaaaatgct cggcattctg cttctgatct tcgcaattga
     1741 ctgtgctttt gcagtattca tgatctcaaa gggctacaga acatttcctc atctcatcgg
     1801 attatcaagg ccgcttattt tcatgtacgg acctcttttc tttctcatat tttcaatcgc
     1861 tactggctat cattcaaggg taaagacaga ccacctgttt catttcatcc cttttctcac
     1921 ccacttttct cttatgacac ctttcatttt caaaactcct gcaacaaaaa tattccttta
     1981 tgagcactat gtaaaaggtg ttgcctacat tgaatactat ccccacgcca tgatgttcct
     2041 gaccgtcatc ttctttttaa tgtactcggt tctgccgctt gcactcactt acaaatataa
     2101 aaagaccgta atgggtttct attcgactat tgacacaagg aaaatcttaa aactttatct
     2161 gttttcagct ctttacatca tttgtgcaat atatctgaca tttgttttcc acggatatct
     2221 gacaggaccg tctttcattc gtcccgacat tccgttccat tttctttact tccttgccgc
     2281 cattatattt cctttcacct tcatggcgat aaccgatctt tcattcctca gaccatctct
     2341 ttcaatacat gaccttatat ttgaagatga accggctgaa aaaaccgaca gcgaagaaaa
     2401 gactgaagta tcaactgctg aaaaatacca gaaaaataag atgccggatg aacttgcaca
     2461 gaaagcattt gaacttttaa aagaggttat ggctgaaaaa aacccgttca ccgatagtaa
     2521 tcttacactt gtcgatcttg cagaaatgat acacatcagg ccttatcacc tttcacagct
     2581 tatcaacagc agaacaggcg acaattttta tgttttcatc aactcggcaa ggataaaata
     2641 cgccgcggaa cttttaaaag atcccgtcca caaagaaaaa agtatccttg aaataatata
     2701 tatgtccggc tttaattcaa aaagcgcatt caacacctat ttcaagaaca tcatgggcga
     2761 aactcccagt aatttcagga aaaaacactc gtaatttcag gttcttcctt atgtgtcaga
     2821 acttttaaac tcttcaatat ttcaagcgat ctgttcttcc tcatgtgaca ggacgaataa
     2881 gcagaaaaaa tcatttaatc tgaaaatgta accgggcttt ttatcaaccg aacgaatgga
     2941 gagtgtcatg agaaaaatta ttgcaatttt aaacatttta acagttttcg ctttttcgtg
     3001 ttcaacacct gaaaaggcgg aaattgctga ggt
//
LOCUS       DAOOYB010000327         2990 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_40272_length_2990_cov_8.956729, whole genome shotgun sequence.
ACCESSION   DAOOYB010000327 DAOOYB010000000
VERSION     DAOOYB010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40272_length_2990_cov_8.956729"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(87..422)
                     /locus_tag="PL056_12900"
     CDS             complement(87..422)
                     /locus_tag="PL056_12900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HPM47960.1"
                     /translation="MKQILYLMILMIVVLISSCVPEDEDGEPDPDDVVTFADYNLERC
                     VRDEMSEGSSGEVLYYKDVFWLSWLRCAGYGIEKLDGIENMKNLREAEFGNNKIKDVT
                     PLGKCVNLR"
     gene            complement(424..2181)
                     /locus_tag="PL056_12905"
     CDS             complement(424..2181)
                     /locus_tag="PL056_12905"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HPM47961.1"
                     /translation="MKYFRVLSVLFFFVLFIASCGEDKKVQQIPDNEETSTDSDAEEL
                     IDNYEHANDNETQETDDTVSETDEDINDEEPLETVEFDIPDPAVENHEAVIVTKDEFA
                     QIFSEMAAFHTMTGISTKVITIETICAGTLCNDEDPKKDTAKAVKDHIRFIPGLKYLI
                     LGGDIEVVPSRKVHDKYSNVMAGTFEDDFQSDFYYADFSDWDSNGNGIYAEDADILDY
                     SAEIAVGRIPVSTVEEALVYFEKLIKHNTDYNTSHVKSSLLIANVATNFSGIDINAGY
                     YFETEGRTRDIIPFDFFKRKLYTKTFPSPANDAEPLNNDVMKQALEGGVNVVVHNGHG
                     YPTLLSCEQANNDNNFTSEMAYALKNTTFPIFLSCACQAGQFEAPFEYTYEYNGETKT
                     RVFTDDAAGEKLVNAPEGGSVFYLGNAITGLGLAGGSQFIDEMLRDMFTKPFSVMGQS
                     YLFAHNALKKNDTFAPPIPAVPAVPVVDEDSWKWTKKGVVALGSPLVTFWRDPFPPIN
                     AVIGVTSEKVMNGYSFTLNIPDELAGFDLRIYSNGKFYTLSAVMPGTETLNIEGEDSN
                     ILFALLKPDHQPFFQKTGL"
     gene            complement(2191..2931)
                     /gene="tpiA"
                     /locus_tag="PL056_12910"
     CDS             complement(2191..2931)
                     /gene="tpiA"
                     /locus_tag="PL056_12910"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013638193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HPM47962.1"
                     /translation="MRKPIIAANWKMNNLTADITKFFNEFADTANLFKDRDAIIAPSF
                     PYLGMCAELAKGKNITIAAQDVYPAEEGAFTGMVGSRMLNDCGVGAVIVGHSERRHIF
                     GESLELIRKKVAFVQNNNWKLIFCIGETLPERESGRLYEIIKEQIYTGFDENLWCEND
                     MAVIAYEPVWAIGTGVVATPEQAEEMHAFIRKIMKERYNREEIRILYGGSVKPDNIKG
                     LMSCENIDGALVGGASLKADSFKKLVEF"
BASE COUNT          801 a          711 c          536 g          942 t
ORIGIN      
        1 ttttctgctc atttaattag aaccttgttt attcacaccg actgtaatcc tgatcgtcag
       61 aggagtcgcc caatatattt actaatttac ctcaaattca cacatttccc caatggtgta
      121 acatccttta tcttgttgtt gccgaattcg gcttcacgca gattcttcat gttctctatg
      181 ccatccagtt tttctatgcc gtagcctgca cacctcaacc aagacaacca gaaaacatcc
      241 ttataatata atacttcccc tgaactgcct tctgacattt catccctgac acatctctca
      301 agattgtaat ctgcaaatgt tacaacatca tcaggatcag gctcaccgtc ttcatcttca
      361 ggaacacagg aacttataag aactactatc ataaggatca ttaagtatag tatctgtttc
      421 atctcacaat cctgtcttct gaaaaaacgg ttgatggtct ggctttaaaa gtgcgaaaag
      481 aatattgctg tcttcccctt cgatgttcaa tgtttcggtt cctggcatta ctgctgaaag
      541 tgtgtagaac tttccgtttg aataaattct gagatcaaag ccggcaagtt catcaggaat
      601 gttgagcgta aaagagtatc cgttcatcac tttttctgaa gtgacgccaa ttacagcgtt
      661 aattggggga aacggatctc tccagaaagt cacaagcgga ctgccgaggg cgacaacccc
      721 tttttttgtc catttccagc tatcttcatc aactacagga acggcaggaa cagccggtat
      781 gggaggagcg aaagtgtcat tttttttcag tgcgttatgg gcaaaaagat aactctgccc
      841 catcacagaa aacggttttg tgaacatgtc gcggagcatt tcatcaatga actggcttcc
      901 acctgcaaga ccgagacctg taatggcatt tccaagataa aagaccgatc cgccctctgg
      961 agcattcaca agtttttcac ctgccgcatc atcggtaaac accctcgttt ttgtctcccc
     1021 gttgtattca taggtatatt cgaaaggtgc ttcaaactgt cctgcctgac acgcacagct
     1081 taaaaatatc gggaatgttg tattctttag cgcatacgcc atctcggaag tgaaattatt
     1141 gtcgttgttc gcctgctcac agcttaaaag tgtcggatat ccgtgaccgt tatgaacaac
     1201 tacattgacg cccccttcaa gtgcctgttt catcacatcg ttattgagcg gctcggcatc
     1261 atttgcagga gatggaaatg tctttgtata aagctttctc ttgaaaaaat caaatgggat
     1321 tatatctctc gttctccctt cagtttcaaa gtaatatccg gcatttatat caattccgct
     1381 gaaatttgtc gcaacatttg cgatcagaag tgaacttttt acatgactgg tgttgtaatc
     1441 tgtattgtgt ttaataagtt tttcaaaata aactaacgct tcctcaacgg ttgaaaccgg
     1501 aattctcccc acagctattt cagcagaata atcaagaata tcagcatctt ctgcatatat
     1561 tccattgccg tttgaatccc agtcactgaa atcggcataa taaaaatcgc tttgaaaatc
     1621 atcttcaaaa gtccctgcca ttacatttga atacttgtca tgaacctttc ttgaaggaac
     1681 gacctcgata tcaccgccga gaatcagata tttcagaccc gggatgaacc ggatgtgatc
     1741 tttcaccgct ttcgcagtat cttttttcgg atcttcatca ttgcacagtg ttccggcaca
     1801 tatcgtctca atcgtaatga cttttgtgct tatccctgtc atcgtgtgga aagccgccat
     1861 ttcgctgaag atctgtgcaa actcatcttt tgtcactatc accgcttcat gattttcaac
     1921 agccgggtcc ggaatgtcaa actcgacagt ttcaagcggc tcttcgtcat ttatatcttc
     1981 atcagtttca gaaacggtgt catcggtttc ctgtgtttcg ttatcatttg catgctcata
     2041 gttgtctatc agctcttcag catcggaatc ggttgaagtt tcttcattat caggaatctg
     2101 ctgtactttt ttatcttcac cgcatgaagc aataaaaaga acaaagaaaa aaagaacaga
     2161 aagaacacga aaatatttca tatcaccacc ttagaactca acaagttttt taaatgaatc
     2221 ggctttcaag cttgctccgc cgacaagagc gccatcaata ttttcacaag acataagtcc
     2281 tttaatatta tccggtttta cgcttccgcc ataaaggatt ctgatctctt cgcggttata
     2341 tctttccttc attatcttcc ttataaaagc gtgcatttct tctgcctgtt ccggagttgc
     2401 cacaacaccc gttccgatcg cccagaccgg ttcgtatgcg atcacagcca tatcattttc
     2461 acaccagaga ttttcatcaa agccggtgta tatctgctct ttaattatct cataaagccg
     2521 gcccgattcc ctttcaggaa gagtttcacc gatgcagaaa ataagtttcc agttgttgtt
     2581 ctggacaaaa gcgacctttt ttcttatcag ttcaagagat tcaccgaata tgtgccttct
     2641 ttcgctgtga ccgacaatta cagcacccac tccgcagtca ttgagcattc tactgccgac
     2701 catccccgta aaagccccct cttctgccgg atagacatcc tgggcggcga tagttatgtt
     2761 cttccctttt gcaagttcag cgcacattcc aagataagga aatgacggtg ctataatagc
     2821 atccctgtct ttaaaaagat ttgctgtgtc tgcgaattca ttgaagaatt tcgttatgtc
     2881 agcagtaaga ttgttcattt tccagtttgc cgcgataata ggttttctca ttgatccctc
     2941 ctacgaataa ttggttataa tttttttgta tctcagactt gttctttgat
//
LOCUS       DAOOYB010000328         2984 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_40390_length_2984_cov_9.763059, whole genome shotgun sequence.
ACCESSION   DAOOYB010000328 DAOOYB010000000
VERSION     DAOOYB010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40390_length_2984_cov_9.763059"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            226..1545
                     /locus_tag="PL056_12915"
     CDS             226..1545
                     /locus_tag="PL056_12915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47963.1"
                     /translation="MFRKFFSVSIILGLFFSVHAAEVTEVASSFDYKNPFDFNLDVKY
                     LSTQHFGSIKREYNTMIDGAYQDVNGNLYYATTDRRVETKEYEGNPHLMHMQKLLFDA
                     EIGLYHNLSLSLQLPVILSEQYKMVLFDTSVNPDDSTLAGQQLFPWAGNLDYTHKGLG
                     DLSIGLQWAPFSEERKNRFMSWLLGMNVTFPTAPVKTPEGMNKLTRSSGTNYIVPSGN
                     AGKAGDGLFRFDFRTAVSKKYSITEPYFQFMFSLPVNSSKSIYNDPKEAVVFNIGSDF
                     NFWENKKHGQRVKMRTDIEVAYTNKGNAYNAIADARAVSDDNWVANPDSLNYPWPFTT
                     NESKNRNILPVEDSWVQISGMLKLSVRVYKYVELGGFAKFGYRHKHYLTNANPGTAGY
                     IEGLDSEKTSWLSDSKKDSDPRSRLGGRLLAEQYLMLDWGFNLKLLF"
     gene            1552..>2984
                     /locus_tag="PL056_12920"
     CDS             1552..>2984
                     /locus_tag="PL056_12920"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HPM47964.1"
                     /translation="MNLQGKKILFARICGTGVSSLALICKNAGAIVTGVDLDYYPPVS
                     TKLAEENIPCFAMDKFFELIENDRPDIIVVGNALNGKSAEAKFILEGDIPYYSMPSFM
                     EEFLLDSKKPVVISGTHGKTTTTTIFSELLTGIGADPSYMIGGIPEFSGTNSAFSEGE
                     KYFVIEGDEYDTAFFDKGPKFLHYKPETTIITSIEFDHADIYDSVDAIFNNFVKLVGI
                     TKKKVILCLDFEHNRNLTCHIPSEKLFTYSIEDKRADAFIQFEEREGSLMVFSVNFKN
                     GKRYSFKSPMFGMQNLMNLGSLIAFLKCEGFDLKDKKLHEVIGNMKGIKRRQELIGSI
                     NGGLVYSDFAHHPTAAKLTLESFLQFFPEKKVSVIFDPATNSNNQNVFEGEYTEIFKA
                     AHKFVLGHPPKLEKFPPELRFSPERVVLGVNSEGVEKAKYIKEVDDVINWLRENSTPE
                     TVTVVMSNSGFYGFFTKIIPYLDKESK"
BASE COUNT          928 a          580 c          614 g          862 t
ORIGIN      
        1 caaaatgtcc attatccgat tacacaccga acacagaaaa tcccaaaaaa ccttggggat
       61 gggactggaa accgggagag tgaaaaaaat acctgtgaca gatacaacac taattgctct
      121 cactatcagt catattcatt tactgaaatt ttataatatc taaaacttga ctactaaacg
      181 gacattttct atactctttc aaaacggatt ccggaggatt gctctatgtt cagaaagttt
      241 ttttcagtct cgataatatt gggtttgttc ttttcagttc atgcggctga agtaactgag
      301 gttgcaagtt catttgacta caagaatcct tttgatttca atcttgatgt caaatatctt
      361 tcaacgcagc atttcggctc gataaaaaga gaatacaaca ctatgatcga cggtgcatat
      421 caggatgtga acggcaatct ttattatgca acgaccgata gaagagttga aacaaaggaa
      481 tatgagggaa atccgcatct tatgcacatg cagaaacttc tttttgatgc agagatcggg
      541 ctttatcaca acctttctct ttcgttacag cttcctgtaa ttctttctga acagtataaa
      601 atggtccttt ttgataccag tgtaaatcct gatgattcga cgctcgcagg acagcagctt
      661 ttcccatggg ccggaaatct ggattacact cacaaaggtc tgggtgatct ttctatcggt
      721 cttcaatggg ctccgttcag tgaagaaaga aagaacaggt tcatgagctg gcttctcggg
      781 atgaatgtca ctttcccgac agctcctgtc aaaactcctg aagggatgaa taagctcacc
      841 agaagttccg gtactaacta cattgtccca tcaggaaatg caggaaaggc gggtgacggt
      901 cttttcaggt ttgatttcag gacagcagtt tccaagaaat attcaataac ggagccgtat
      961 ttccagttca tgttctctct tccggtaaat tcatcaaagt cgatatataa cgatcctaaa
     1021 gaggcggttg tttttaatat cggttccgat ttcaatttct gggagaacaa aaaacacggt
     1081 cagcgtgtaa aaatgagaac agatatcgaa gttgcatata caaacaaagg taacgcatat
     1141 aacgcaatcg ccgatgcaag agcagtaagc gatgacaact gggttgcaaa ccctgacagt
     1201 ctcaactatc catggccttt tactacgaat gaaagcaaga accgcaacat tcttcctgta
     1261 gaggattcat gggttcagat atcaggaatg cttaaacttt cagtgagagt ttataagtat
     1321 gttgaactgg gcggttttgc gaagttcgga tacaggcaca aacattatct taccaatgcg
     1381 aaccccggca cagcaggata tatcgaaggt cttgatagcg aaaagacttc atggttatct
     1441 gattcaaaaa aagatagtga tccccgaagc agattgggtg gaagacttct tgctgaacag
     1501 tatctgatgc ttgactgggg atttaacttg aaacttcttt tctgatcagt tatgaattta
     1561 caaggaaaga aaatactttt cgcaaggatt tgcgggactg gtgtaagttc tctcgccctg
     1621 atctgtaaaa acgcgggagc aatcgttacc ggtgttgatc ttgattacta tcctccagtc
     1681 agtaccaagc ttgcagaaga gaacattccg tgctttgcca tggacaagtt ctttgaattg
     1741 attgaaaatg acagacctga cataattgtt gtcggaaacg cacttaacgg aaaatcagct
     1801 gaggcgaagt ttattcttga aggtgacatt ccttattatt caatgccttc tttcatggag
     1861 gagttccttc ttgacagtaa aaaacctgtc gttatttcag gaactcacgg caaaacgacg
     1921 accacgacga tcttcagtga acttttaacc ggtattggag ccgatccttc ttacatgatc
     1981 ggcggtattc ccgagttttc cggcacgaat tccgcttttt ctgaaggtga aaaatatttt
     2041 gttatagaag gtgatgaata cgacacagct ttttttgata aagggccgaa attccttcat
     2101 tacaagccgg aaacaacaat tataacatcg atagagttcg atcatgccga tatttatgat
     2161 tcggttgacg ctattttcaa caacttcgta aagcttgtcg gaattacaaa gaaaaaagtc
     2221 attctctgtc tcgattttga acacaacagg aaccttacat gccacatacc ttctgaaaag
     2281 cttttcacat attccattga agataaaagg gctgatgcgt tcattcagtt tgaggaaaga
     2341 gaaggatctt taatggtgtt ctcggttaat ttcaagaacg gaaaaagata ctcgttcaaa
     2401 tctcctatgt tcgggatgca gaatctcatg aatctcggct cgctcattgc ttttcttaaa
     2461 tgtgaagggt ttgaccttaa agataaaaaa ctacatgaag tgatcgggaa catgaaaggg
     2521 ataaaaagaa ggcaggagct tatcggttca ataaacggcg gtcttgttta cagcgatttt
     2581 gcacaccatc cgactgctgc aaaacttacg cttgaatcat ttcttcagtt cttccctgaa
     2641 aaaaaagtgt ctgtaatttt tgatcctgcg acaaactcaa ataaccagaa tgtttttgaa
     2701 ggtgagtaca ctgaaatttt taaagcggct cacaaattcg ttcttggaca tccgccgaaa
     2761 cttgaaaaat ttccaccgga actccggttt tccccggaaa gagttgtctt gggagtaaat
     2821 tccgaagggg tagaaaaagc aaaatatatt aaagaagttg atgatgttat aaactggctt
     2881 agagaaaaca gcactcctga aactgtcaca gttgtcatgt caaattcagg gttttacggt
     2941 ttttttacaa aaataattcc atatttagac aaagaatcta aata
//
LOCUS       DAOOYB010000329         2967 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_40728_length_2967_cov_14.424794, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000329 DAOOYB010000000
VERSION     DAOOYB010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40728_length_2967_cov_14.424794"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..116)
                     /locus_tag="PL056_12925"
     tRNA            complement(40..116)
                     /locus_tag="PL056_12925"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(80..82),aa:Asp,seq:gtc)
     gene            complement(138..213)
                     /locus_tag="PL056_12930"
     tRNA            complement(138..213)
                     /locus_tag="PL056_12930"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(178..180),aa:Val,seq:tac)
     gene            complement(301..549)
                     /locus_tag="PL056_12935"
     CDS             complement(301..549)
                     /locus_tag="PL056_12935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47965.1"
                     /translation="MLDSRILLKSLNDLEVRIMLCKSAFEWELLAKKYVDVESNIKDF
                     FKKKKIPENFATALDTLRASLVQKKGELPPVDLSDFFK"
     gene            complement(563..1513)
                     /locus_tag="PL056_12940"
     CDS             complement(563..1513)
                     /locus_tag="PL056_12940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47966.1"
                     /translation="MGLIKAVADAVMEKAKHEVKDYLIKFMEEEKEKEKKTEEIKKEE
                     IPAAVLKESVKAEELKEEDDEDEDEDEDEEILPLIESEFTKFTYKVKPKDFTIPGELA
                     DIIKMLPVEEFACFVKLMIFVFEQKKNFGYIGNTLRRKIGLESFENEKFDKTVENLEK
                     YGVLKVEQVSDNQKTFILYVPFDEETMVKAEYQKPEKTQQKKVEKKPVKKGDEEEKPV
                     EKQNDQQDDIPGKAVFGMENDDLYKSYRTFVSLEIDKAKMRVGRSNFDKIYMEAVKYI
                     DKKYGFKVLSDSEKFKEYLTNYYISAFDIPTFETWKKTKV"
     gene            complement(1526..1903)
                     /locus_tag="PL056_12945"
     CDS             complement(1526..1903)
                     /locus_tag="PL056_12945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47967.1"
                     /translation="MKRTVLIIIVIMAAVITLYAKETKKASSEAEVSWNRTEKGVSYA
                     ITFDREYKINRSAPFNFYLLDKDKKEITKVKWDAFAKENDLKYVFVSDKKESFAKYWF
                     VACKYQNNEVVSCKTFSNTIEIK"
     gene            complement(1900..2304)
                     /locus_tag="PL056_12950"
     CDS             complement(1900..2304)
                     /locus_tag="PL056_12950"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HPM47968.1"
                     /translation="MEIKRILELRARMEKALAAYLGGRGDNPFDNRDIGYINPSADVY
                     IDGRRQLIFIETPAMIEDSVKVDIIDDNLVFSSGKTVSRHTGRKYLQIERKIGSYTKR
                     IPLAFKESEVVSVKHSYKLGVIKIEIEYGEKK"
     gene            complement(2389..>2967)
                     /locus_tag="PL056_12955"
     CDS             complement(2389..>2967)
                     /locus_tag="PL056_12955"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HPM47969.1"
                     /translation="GEYGEKWHQSGMLDKKQNTFDDFGWAMKYLTDNKYTSPEKLAIW
                     GGSNGGLLTGAMVTQYPELFKAAVVAVPLLDMLRFHKFLIGRYWVSEYGSSDNEEQFR
                     YILKYSPYHNIKKEGKFPAALITAGEHDSRVHPMHAKKMVAALQFENRSDNPIFLWVD
                     TKAGHGQGKSTQQRIKESAMELGFFLRMLGVN"
BASE COUNT          755 a          669 c          513 g         1030 t
ORIGIN      
        1 gatgatagca gaaaaaatta agatttgaag gataaaaaat ggcgcggtcg accgggttcg
       61 aaccggcggc ctccgacgtg acaggccggc gttataaccg acttaactac gaccgcgcct
      121 caatctcgaa agattgatgg tgggcgctgc agggctcgaa cctgcgacca ccggcttgta
      181 aggccgatgc tctcccagct gagctaagcg cccatcaaca aatgaggttt atacttacgg
      241 cttcatattt gtcaaagaaa aaaatgtatt tgaaacatca gctgttataa ttgcctgaaa
      301 tcacttaaaa aaatcagaca gatccactgg aggcagttcc cctttctttt gcacaagaga
      361 cgctctcagc gtgtcaaggg cagtcgcaaa attttcagga atcttctttt tcttgaagaa
      421 atcctttata ttcgattcaa catctacata tttttttgca aggagctccc attcgaaagc
      481 acttttgcac agcattatcc ttacttcaag atcatttaat gatttcaata atatccggct
      541 gtcaagcata actcaaactc catcacactt ttgttttttt ccaagtttca aatgtcggta
      601 tgtcaaatgc cgaaatatag taatttgtga gatattcctt gaatttctcg gaatctgaaa
      661 gcactttgaa cccgtatttc ttgtcaatat acttgaccgc ttccatatat atcttgtcaa
      721 aattcgacct gccgaccctc attttagctt tgtcaatttc aagagatacg aaggttctgt
      781 acgatttgta aagatcatca ttttccattc caaagactgc ttttcccgga atatcatcct
      841 gctgatcatt ctgtttttca acgggctttt cttcttcatc acctttttta accggcttct
      901 tttcaacttt tttctgctgg gttttttcgg gtttctgata ctcggctttt accattgtct
      961 cttcatcaaa gggaacatag agaataaaag ttttctggtt gtctgaaacc tgctcaactt
     1021 tcaaaacacc gtatttttca aggttttcaa ctgttttatc aaatttttca ttttcaaatg
     1081 actcaagacc tattttcctt cttaaagtgt tgccgatgta tccgaagttc tttttctgct
     1141 caaaaacaaa tatcatgagc tttacaaaac atgcaaactc ttctaccgga agcattttta
     1201 ttatgtctgc aagttcaccg gggatagtaa aatctttcgg tttgaccttg tatgtgaact
     1261 ttgtgaattc ggattctatc aggggaagga tctcttcatc ttcatcttca tcttcatctt
     1321 catcatcttc ttctttgagt tcttcagctt taactgattc tttcagcact gctgccggaa
     1381 tttcttcctt tttgatctcc tctgtttttt tctctttttc cttttcctct tccatgaact
     1441 ttataaggta atccttgacc tcatgcttag ctttttccat tacagcatca gcaactgctt
     1501 ttataagacc cattttaccc tccggttatt taatctcaat tgtatttgaa aaggttttgc
     1561 aggaaaccac ttcgttgttc tgatatttgc atgctacaaa ccagtatttg gcaaaactct
     1621 cttttttatc tgacacaaaa acatatttca gatcattttc cttagcaaac gcatcccact
     1681 taacttttgt tatctccttt ttatccttgt caagaagata aaagttaaac ggagccgatc
     1741 tgtttatttt gtattctctg tcaaatgtga ttgcatatga tacgcccttt tccgttctgt
     1801 tccacgaaac ttcagcttca cttgatgcct tctttgtctc ttttgcataa agcgtgataa
     1861 cagccgccat tatcacgatg atgatcagca ctgttctttt catttttttt caccatattc
     1921 tatttcaatt tttattacgc cgagtttgta actgtgtttt actgaaacaa cttcagattc
     1981 tttgaacgca agaggaattc ttttcgtata cgagccgatc ttcctttcga tctggagata
     2041 ttttctgccg gtgtgccttg aaactgtttt tccggaagaa aagaccaggt tatcgtcaat
     2101 gatgtcaact ttgactgaat cttcgatcat agcaggggtt tcgatgaata tcaactgcct
     2161 tcttccgtca atatagacat ctgccgaagg attgatgtaa cctatatctc tgttgtcaaa
     2221 aggattgtca cctcttccac ccaaatatgc cgccaaggcc ttttccatcc ttgctctcag
     2281 ctcaagtatc cttttgattt ccatgaccgc tcctcttaaa aagaccagaa caatattaag
     2341 gacatagtta taaaagtcaa tatttagctc ttaaaaaaac cccgaaagct agtttacacc
     2401 gagcattctg agaaagaagc caagttccat ggctgactct tttatccttt gctgagttga
     2461 ttttccctgt ccgtgtcccg cttttgtgtc aacccagaga aatattggat tgtccgatct
     2521 gttctcaaat tgtaaagcgg cgaccatttt ctttgcgtgc atggggtgaa ctcttgaatc
     2581 gtgttcccct gctgtgatga gggcggcagg aaatttacct tcttttttta tgttgtgata
     2641 aggggaatat ttgagaatat atctgaactg ttcttcgttg tcacttgaac cgtattcgct
     2701 tacccagtat cttccgatga ggaacttgtg gaatctcagc atgtcaagaa ggggaactgc
     2761 gacaactgcc gccttgaaaa gttcaggata ctgagtcacc attgcacctg tcagcagtcc
     2821 gccgttgctt ccgccccaga tcgcaagttt ttcagggctt gtatatttat tgtcggtcaa
     2881 atatttcatc gcccagccga aatcatcaaa ggtgttctgt tttttgtcga gcattcctga
     2941 ctggtgccac ttttcaccat attctcc
//
LOCUS       DAOOYB010000330         2924 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_41603_length_2924_cov_3.643081, whole genome shotgun sequence.
ACCESSION   DAOOYB010000330 DAOOYB010000000
VERSION     DAOOYB010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41603_length_2924_cov_3.643081"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2177
                     /locus_tag="PL056_12960"
     CDS             <1..2177
                     /locus_tag="PL056_12960"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HPM47970.1"
                     /translation="FSYLKVEQGIKENMKLSLNDNYRSTPEIIDGLKDLFSINKDPFN
                     IEGRIKFIDVKPTEKVTEKLVDNDKKALNIWGVPAKKSAETIIHKNIVSEIKRLLKDA
                     KIGDKALKPSDIAILVTSNKESGTLKEMLLEAGVPAIISSSASVFNSDAAKDLMLFLN
                     AVIDNRNLSLIKGALFTMFFSKSTAECAALDSDSTLADEWLLKFESWHELLNEKGFLK
                     MFSTASKGEEFENKIAGLIDAERNITNLRHISELIVEYEKKNSATPSQILDFLIERSS
                     KDAESADEYEQRLDSERDRVIISTIHKSKGLQYNIVFIPSLYKGPRDIKGAVCNYHDE
                     NDDPVVSLFKTEDEKETVRTEELQEKLRLCYVALTRAVHRAYFVVVNLTRGYNMSVPR
                     HLFNCGFSEIKGKVNGHKNISYKDIENPSDDGKKYEEEKREKVETKSSLEFKRDIGVP
                     FKISSYSALAKKKTVSDEVDMEVITDDAAADVENEVEGSGIFAFKKGAFAGIALHKLF
                     EIVDFSKEDLSDDVKAVLEKHNLLKKSETEEWTEDVVKMVKNVLSAKLKGEFSLSEIR
                     GEQRFNEMEFLFPVKSIKPAKVAEILAPLKISVDFDSMEGFLNGFIDLVFEKDKTYYI
                     VDWKSNHLGNSYKDYSNNHLDKAMKKANYDLQYIIYTAAIHLHLSKTIENYDYDSHFG
                     GIFYIFLRGVDEKGENGIYYKKPEKKIVEQLADYFQEVKNEQ"
     gene            2167..>2924
                     /locus_tag="PL056_12965"
     CDS             2167..>2924
                     /locus_tag="PL056_12965"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPM47971.1"
                     /translation="MSSDIRKKIKETKVFPNIDIHFANFIIEENGEWCSELYFTALFL
                     SKGLMSGHIAIEPENLNIPEELKEIDVPERKGWIEKIKECAIIKNEISPVIIDKNRIY
                     LQRYHYYESSLADLIKNKAVKKDPLHYDNKNMGKFFKDDTIKKEAAEHVMKNGLTVIT
                     GGPGTGKTTVVANILHLLVSKDPDLRFALAAPTGKAAARMTEAIKNSFQRCFEEKDPV
                     CATILKHKGSTLHRLLGYNRNSVEFKHNEKKRLP"
BASE COUNT         1072 a          485 c          588 g          779 t
ORIGIN      
        1 ttttcagtta tctaaaggtt gagcagggaa tcaaggaaaa tatgaaacta agtctcaacg
       61 acaattacag atcaactcct gaaataattg atggattaaa agatcttttt tccataaaca
      121 aagatccttt taacattgaa gggagaatta agtttattga tgtaaaaccg acagaaaaag
      181 taactgaaaa gcttgtcgat aatgataaaa aggctcttaa tatatggggc gtccccgcta
      241 agaaaagcgc agagaccata attcacaaaa acatcgtttc agagataaaa agactgctga
      301 aagatgcaaa gatcggcgat aaagctttaa aaccatctga cattgcgatc ttagtaacct
      361 ccaacaagga atccggaacc ctcaaagaaa tgcttttaga agcgggtgtc cctgcaataa
      421 tatcctcatc cgcctctgtg tttaattcag atgcggctaa agatctaatg ctgtttttaa
      481 acgcagtaat tgataataga aacctgagtc tgataaaagg ggctttattt acaatgttct
      541 ttagcaaaag cacggctgaa tgtgcagcac ttgattcaga ttcaacactc gcagatgagt
      601 ggcttttaaa gtttgaaagt tggcatgaac ttttaaatga aaaaggattt ttaaaaatgt
      661 tctcaactgc ttctaaaggt gaagagtttg aaaataagat cgcaggactg atagatgcag
      721 aaagaaatat cacaaatctt agacatatat ctgaacttat tgtggaatat gaaaagaaaa
      781 acagtgcaac tccgtcacag atactggatt ttcttattga aaggtcctca aaagatgctg
      841 aatccgctga tgaatatgaa cagcggcttg attcagaaag agaccgggtg ataatatcaa
      901 caatacacaa gagcaaagga cttcaatata atattgtttt tatcccgtca ctttataagg
      961 gacccaggga tattaaaggt gctgtgtgta actaccacga tgagaacgat gatccggtcg
     1021 tttctctttt caaaacagag gatgaaaagg aaactgtaag aacagaggag ctgcaggaaa
     1081 agctccgtct ttgctatgtc gcccttacga gggccgtaca cagggcatat tttgtggttg
     1141 tcaatcttac acgaggatat aatatgtctg ttccaagaca cttatttaac tgcgggttca
     1201 gcgagataaa gggaaaagtg aacggtcata aaaacataag ctataaagat atcgaaaacc
     1261 cgtccgatga cgggaaaaaa tatgaagagg aaaaaagaga aaaggttgaa acaaaaagct
     1321 ctctcgaatt taagagagat atcggcgttc cttttaaaat atcaagctat tcagctcttg
     1381 caaaaaagaa aaccgtatca gatgaagttg acatggaggt cataacagat gatgcggcgg
     1441 ctgatgttga aaatgaagtt gaggggagcg gaatctttgc tttcaaaaaa ggggcttttg
     1501 caggaattgc ccttcataaa ctctttgaaa tcgttgattt ttcaaaggaa gatctaagtg
     1561 atgatgttaa agcagttctt gaaaaacaca acctcttgaa aaaatcagaa actgaagagt
     1621 ggacggaaga tgttgtaaaa atggttaaaa atgtactttc cgcaaaactg aagggagaat
     1681 tttcactttc tgaaatcagg ggggagcaga gattcaacga aatggaattt ctttttcctg
     1741 taaaatcaat aaagccggca aaggtcgcag aaatattagc tccgcttaaa atatcagtcg
     1801 attttgactc catggaaggg tttttaaatg gttttataga tcttgtattt gaaaaagata
     1861 aaacatatta catagttgac tggaaatcaa atcatcttgg aaacagttat aaggactaca
     1921 gcaataatca tcttgataaa gcaatgaaaa aggcaaacta tgatctccaa tacataattt
     1981 atactgccgc aattcatctt catctttcaa aaacaattga aaactatgat tatgacagcc
     2041 atttcggcgg aattttctat atttttctac ggggcgttga tgaaaaagga gagaacggaa
     2101 tatattataa gaaacctgag aaaaaaatag ttgagcaact tgccgattat tttcaagagg
     2161 tgaaaaatga gcagtgatat cagaaaaaaa attaaagaaa cgaaagtgtt tccaaatatt
     2221 gatatccatt tcgccaactt cataattgag gagaacggtg agtggtgttc cgagctctat
     2281 tttacagcac tttttctaag taaggggctg atgagcggtc acattgcaat agaaccggaa
     2341 aatctcaaca ttcctgaaga acttaaagaa attgatgttc ctgaaagaaa gggatggata
     2401 gaaaaaatta aagaatgtgc cataattaag aatgaaatat cacctgtaat cattgataaa
     2461 aaccgtattt atcttcagag ataccactat tacgaatctt ctcttgcaga tctgataaaa
     2521 aataaagcgg tcaaaaaaga tcctctgcat tatgataaca aaaatatggg gaaatttttc
     2581 aaagatgaca ctataaaaaa agaagccgcg gaacatgtta tgaaaaacgg cctgactgtc
     2641 ataaccggcg gtccaggaac cggcaaaaca acagttgttg cgaatattct ccaccttctt
     2701 gtttcaaaag atcctgatct ccgctttgct cttgctgctc cgacaggaaa agccgctgca
     2761 agaatgacag aagcgataaa aaactctttt caaagatgtt ttgaagagaa ggatcctgtt
     2821 tgtgcgacca tccttaaaca caaaggatcc actctccaca gacttcttgg atataataga
     2881 aattcagttg aattcaagca taacgagaaa aaacggcttc cttt
//
LOCUS       DAOOYB010000331         2899 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_42100_length_2899_cov_8.812236, whole genome shotgun sequence.
ACCESSION   DAOOYB010000331 DAOOYB010000000
VERSION     DAOOYB010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42100_length_2899_cov_8.812236"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..244
                     /gene="rpmG"
                     /locus_tag="PL056_12970"
     CDS             68..244
                     /gene="rpmG"
                     /locus_tag="PL056_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HPM47972.1"
                     /translation="MGSKKKVGARVKVSMSCSECKRKNYTTVKNKKNTADKIEMKKYC
                     PYDKKHTVHKEGKV"
     gene            254..329
                     /locus_tag="PL056_12975"
     tRNA            254..329
                     /locus_tag="PL056_12975"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:287..289,aa:Trp,seq:cca)
     gene            351..734
                     /gene="secE"
                     /locus_tag="PL056_12980"
     CDS             351..734
                     /gene="secE"
                     /locus_tag="PL056_12980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HPM47973.1"
                     /translation="MKNGKLLFGIFAAFFLILLYFMTQAADALLSIRAIDIADTRLLS
                     LVPVKNLAGFIIAAGITIYFWKGKNGFYVEELNKALAELMKVVTPTKDETRVTTISVF
                     VFVGIMIVVFVFFDFLWGNLSSLIY"
     gene            754..1326
                     /gene="nusG"
                     /locus_tag="PL056_12985"
     CDS             754..1326
                     /gene="nusG"
                     /locus_tag="PL056_12985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HPM47974.1"
                     /translation="MKWYAIHTQSGHENKLKTAIENLIKEKGMGDFFGEIIVPEVEVE
                     EKKDGKKKLVRKNLYPGYIFLQMEYTEDALLLIKSIAFARKPLFIGERKRFATGKNKN
                     AVSMPTVITEQEIIKIKKMIEDGEVSTASAVMYEKGEMVLIKDGPFAGFKAVIDDVKE
                     GKEKLDVLVNIFGRSTPVELNFSQVERVED"
     gene            1348..1770
                     /gene="rplK"
                     /locus_tag="PL056_12990"
     CDS             1348..1770
                     /gene="rplK"
                     /locus_tag="PL056_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HPM47975.1"
                     /translation="MAKKVLANIKLQIPAGQANPAPPVGPALGQHGVNIMEFCKSFNA
                     RTQEQKGFIVPVVITVYGDRSFTFITKTPPASQLLMKALNKSKGSGEPNKKKIGTLTW
                     DQIEEIAKVKLEDLNTTDLEAAKQIIAGTARSMGINVK"
     gene            1780..2487
                     /gene="rplA"
                     /locus_tag="PL056_12995"
     CDS             1780..2487
                     /gene="rplA"
                     /locus_tag="PL056_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019213923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HPM47976.1"
                     /translation="MANYGKRMKDAMNNFDRNVKYSIKESVAILQKSASKKFDETVEV
                     AVNLGINAKKSDQMVRGATVFPHGVGKTAKVCAVVPADMVELAKNAGADYYGSEELIE
                     KIMTEEWTDFDKLVTTPAMMGKIGKLGKVLGRKGLMPNPKLGTVNNDISAAIKLVKAG
                     RVEFKTNKAGVLCCMVGKLSFGPDKISDNVMELMKDLIKLKPATAKGTFIKKITISST
                     MGPGVKLDETAIVSEAK"
     gene            2599..>2899
                     /gene="rplJ"
                     /locus_tag="PL056_13000"
     CDS             2599..>2899
                     /gene="rplJ"
                     /locus_tag="PL056_13000"
                     /inference="COORDINATES: protein motif:HMM:NF012679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HPM47977.1"
                     /translation="MERSKKQQIIEEIRDVLSKASSCIAVDFNGVKMETFTPLRKECA
                     SKHVKMMVVKNALARIAVKGTEYEGITNFLTGMTSLMFTLDADQVNGAKIVKNFSK"
BASE COUNT          951 a          511 c          695 g          742 t
ORIGIN      
        1 gggaaggcgg cagaactgta ggttcaggcg ttgtgtccaa gataattgaa tagatatagg
       61 tgaaaaaatg ggttcaaaaa agaaagtcgg tgcaagggta aaagtttcca tgagttgctc
      121 tgaatgcaaa agaaagaact ataccactgt aaaaaataaa aagaacaccg cagacaaaat
      181 tgagatgaag aaatattgcc cgtatgacaa aaagcatact gtgcataagg aaggcaaagt
      241 ttagtcgtga gtaaggtcag tagttcaatt ggtagagcac cggtctccaa aaccgggtgt
      301 tgcaggttcg agtcctgcct ggcctgccac tatttgttga ggaataaatt atgaaaaacg
      361 ggaaactgct gttcggtatt ttcgctgcct ttttccttat tctgctgtat ttcatgaccc
      421 aggcggcaga tgcactgctc agcatcaggg caattgatat tgcagatacc agacttcttt
      481 cgctggtacc ggtaaaaaac cttgccggat ttattattgc tgccggcata actatttatt
      541 tctggaaagg gaaaaacggt ttttatgttg aagaactgaa caaagctctt gcagaattaa
      601 tgaaagttgt tactcccaca aaagatgaga caagggttac aacaatttcg gtctttgttt
      661 tcgtaggaat aatgatcgtt gtttttgttt tttttgattt tttatgggga aacctttcgt
      721 ccctgatata ttaaaagcac tgaggaataa tttatgaaat ggtatgcgat acacactcag
      781 tcgggacatg aaaacaaact gaaaacagcc atcgaaaacc ttattaaaga aaaaggtatg
      841 ggtgattttt tcggagagat tatcgttcct gaagtggaag ttgaagaaaa gaaggatggg
      901 aaaaagaagc ttgtcagaaa aaatctctat cccggatata tctttcttca aatggaatat
      961 actgaagatg cgcttctgct gataaaaagt atcgcttttg caaggaaacc cctgtttatc
     1021 ggtgaaagaa aaaggtttgc aacaggtaaa aacaaaaatg ctgtctcaat gccgaccgtc
     1081 ataactgaac aggaaatcat aaaaatcaag aaaatgatag aagacggaga agtttccaca
     1141 gcgtcggcag taatgtacga aaaaggcgaa atggtgctga taaaagacgg cccttttgcc
     1201 ggattcaagg cggttattga tgatgtaaag gaaggcaaag aaaaacttga tgttttggtt
     1261 aatatatttg ggcgttctac acccgtcgag cttaacttct cccaagtgga gagggttgag
     1321 gattaacgtt tttaagagga gtgaacaatg gctaagaaag tgcttgcgaa cattaaactg
     1381 caaattcctg caggtcaggc aaatccggct cctcccgttg gtcctgcact cggacagcac
     1441 ggagtgaaca ttatggaatt ctgtaaatct ttcaacgcaa gaactcagga gcagaaaggc
     1501 ttcatagttc ccgttgtcat aacggtttac ggagacagat cgtttacatt catcacaaaa
     1561 actccgcctg catcccagtt gcttatgaaa gcattgaaca agagtaaagg atccggggag
     1621 cccaacaaaa agaagatcgg taccttgact tgggatcaga ttgaagagat tgcaaaggta
     1681 aaacttgaag atctcaacac tactgatctt gaagccgcaa aacagattat tgcgggaaca
     1741 gcccgcagta tgggaattaa tgtgaagtag gagatcagaa tggcaaatta tggaaaaaga
     1801 atgaaagatg ctatgaacaa cttcgacaga aatgtgaagt acagcattaa ggagtctgtc
     1861 gccattcttc agaaatctgc ttcaaagaaa tttgatgaga ctgtggaagt ggctgtaaat
     1921 ctcgggatca atgcaaaaaa atctgatcag atggtcagag gtgcaacagt attcccgcat
     1981 ggtgtgggca aaactgcaaa agtgtgtgcg gtcgttcctg ctgatatggt ggaactggca
     2041 aaaaatgccg gtgccgatta ttacggcagt gaagaattga tcgaaaaaat tatgaccgag
     2101 gagtggacag attttgacaa acttgtcaca actccggcaa tgatgggcaa gatcggaaaa
     2161 cttggtaaag tacttggaag aaaaggactt atgcctaacc cgaaactcgg aactgtcaac
     2221 aacgacattt ctgcagctat caagcttgtc aaggcaggtc gtgttgaatt taaaacaaac
     2281 aaagcgggcg ttctctgctg tatggtgggc aagctgagct tcggacctga taagatcagt
     2341 gacaatgtca tggagcttat gaaggacctt atcaaactca aaccggcaac agctaaagga
     2401 acatttatta agaagatcac catatccagc accatgggtc ccggtgtgaa acttgatgaa
     2461 acagcaattg ttagtgaggc gaagtaattt aataatcgtt tctggtcgta gaagccgggt
     2521 gccttagggc ttaatttcct gtctgagacc tgtcattgtt cttcgatgac cagaattagt
     2581 aactgatgga gttgcttgat ggaaagaagc aaaaaacaac agatcattga agagatcaga
     2641 gatgtgcttt ccaaagcttc atcatgcatt gccgttgatt tcaacggtgt aaaaatggag
     2701 actttcactc ctcttcgaaa agagtgtgca tccaagcatg taaaaatgat ggttgtgaaa
     2761 aatgctcttg cgagaatcgc tgtgaaaggt accgagtacg aggggatcac gaattttctc
     2821 accggtatga catctctcat gtttacgctg gatgcggatc aggtcaatgg tgcaaagatc
     2881 gtcaaaaatt tctcaaaga
//
LOCUS       DAOOYB010000332         2898 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_42118_length_2898_cov_19.620120, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000332 DAOOYB010000000
VERSION     DAOOYB010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42118_length_2898_cov_19.620120"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            142..1926
                     /gene="sppA"
                     /locus_tag="PL056_13005"
     CDS             142..1926
                     /gene="sppA"
                     /locus_tag="PL056_13005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HPM47978.1"
                     /translation="MGFIKSILTRFFVMLGVLTFIIIVCAVIIVKSLADMKKEVADNS
                     VLFLNTSSIMGDVSTESSFFYLDSFFGNKVSLVDTLIVIRKATADPKITGLFADLSEV
                     PLSYAQVEELRNELILFKKSGKPAYVWSDTFGEISNGTKNYYLASVFDKIYLQPSGML
                     GLNGLSSESMFFKNMLEKLKIEPIGSHRKEYKTYWNMFSEDKYTKEHKESAESLINSI
                     FSKIMADISDSRDIPVEKITEISDSFTLTSADALKSGLVDGIKYKDEAIEEFKNFIKY
                     EKTISVSSYYKISHSDFEISAKSKIAVIELPGSIHRGISDMGPSGNLQSTGSITAVSL
                     LKKAWKDDNVKGVLIRVNSPGGSVIASESIWNQIGIMVKENKKPVVVSMGTVAASGGY
                     YVSMPAQKIFADHNTITGSIGVVLGKMYTREFFNWMGITFDTVKTGKNTDLFSPLTRL
                     EKDQSDYLEKSLDDIYNSFVTRAAENRKMEVAELEKHAKGRVWTGALAKEKGLVDEIG
                     GFMDALEYIKTKSSCEADIAISKYPEPDKFWEMLTGSEDDSPGIIRYFENVFSFFTTF
                     FKNINYIQREIKVQQQESFQLKGDVEIR"
     gene            1927..>2898
                     /locus_tag="PL056_13010"
     CDS             1927..>2898
                     /locus_tag="PL056_13010"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPM47979.1"
                     /translation="MKIYSVGGSVRDEILKEKSSDKDFVVVGATVEEFLKKFPDAKKI
                     GSSFPVFLVDGAEYAFARIEKKSGLGHHGFEIESHPEVTLIEDLKRRDLTINAMAEDI
                     ETGEIIDPFGGMKDLENKRIVHVTDAFAEDPLRVYRVARFASRFYDFSIDKTTIKLMN
                     SLKKELNTLSVERVWAECLKALSSKKPARFFTVLDEAGVLDVHFPELYNLKNVPAGPV
                     EYHPDDADSFHHTISALERVSDIQSGTDPLLSFSVMCHDLGKALTNPDDYPHHHGHDK
                     SGVELIKAFSKRLKVPRKFEEAAVIFAKNHMKISRIFEMTPSKALKLI"
BASE COUNT          945 a          509 c          627 g          817 t
ORIGIN      
        1 ataatgacca gttctgttga aaaaaattcc gggcacgatg aaatagtcca cataaatctt
       61 gagaattgca gataaattaa taaaattaat gtgatatcgt tccttcccac ggttcataat
      121 aacatttaag caggtgtaaa aatgggattc attaaaagca ttttaacaag gttttttgta
      181 atgctcggtg ttttgacatt cataatcatt gtctgtgccg tcatcatagt taagtctctt
      241 gcagacatga aaaaggaagt ggcggacaac agtgttcttt ttttgaacac atcttcgatc
      301 atgggagatg tttccacaga aagcagtttt ttctatttgg attctttttt tggtaataag
      361 gtttcgcttg ttgacacact gatcgttatc agaaaagcga cggcagatcc taaaataaca
      421 gggctttttg ccgatctttc tgaagttcca ttgagctatg cgcaggttga agagcttcgc
      481 aatgaattga tcttatttaa aaaatcagga aagccggcat atgtctggag cgatactttc
      541 ggagaaatat caaacggaac aaaaaactat tatctggcct cggtctttga caaaatatat
      601 cttcaaccct caggaatgct ggggcttaac ggactcagtt cagagagcat gtttttcaag
      661 aatatgcttg aaaaactgaa aatagagccg atcggatcac accggaaaga gtataagact
      721 tactggaaca tgttttctga agataaatat acgaaagaac acaaggaatc ggcagaatct
      781 cttataaatt caatattttc caagatcatg gccgatattt ccgattcaag agatattccg
      841 gttgaaaaga taacggagat ttccgattcc tttacactta catcagctga cgcacttaaa
      901 tcgggtcttg ttgacggaat aaaatataag gacgaagcaa ttgaagaatt caagaatttt
      961 ataaaatatg aaaaaacgat atcagtaagc agttattaca aaataagtca ttcagatttt
     1021 gaaatatctg caaaatcgaa gatcgcagtc attgagcttc cgggttccat tcacagaggg
     1081 atcagcgata tgggcccttc cggcaatctt cagtcaaccg gatcgataac ggcggtttcg
     1141 cttttaaaga aagcatggaa agatgacaac gttaaaggtg tgctgataag ggtgaattcc
     1201 ccgggcggat cagtcattgc gtctgaatcg atatggaacc agatagggat catggttaaa
     1261 gaaaacaaaa agccggtcgt tgtttcaatg gggactgttg ccgcttcagg cggatactat
     1321 gtttcaatgc ctgctcagaa aatatttgca gatcacaata cgattaccgg ttccataggg
     1381 gtagttcttg gaaagatgta cacaagagaa tttttcaact ggatgggtat aacatttgat
     1441 accgttaaga ccggaaagaa taccgatctt ttttctcccc ttacaagact tgaaaaagat
     1501 cagtccgatt atctggaaaa gtcactggat gatatttaca actcttttgt gacccgtgcc
     1561 gctgaaaata gaaaaatgga agttgccgaa cttgaaaaac acgcaaaggg acgagtatgg
     1621 acaggtgctt tggcaaaaga gaaagggctt gttgatgaga tcggcggatt catggatgca
     1681 ctagaatata taaagacaaa atcatcgtgt gaagccgata ttgcaatatc aaaatatcct
     1741 gagccggata agttctggga aatgctcacg ggttcggaag atgattctcc cgggataata
     1801 agatattttg aaaatgtttt ctcatttttc accacttttt ttaaaaacat taactacatt
     1861 cagcgcgaga tcaaagttca gcagcaggaa tcgtttcaac tcaaaggtga tgtcgagatc
     1921 agataaatga agatctattc tgtcggcgga tctgtaagag acgaaattct caaagaaaag
     1981 tcatctgaca aagattttgt tgttgtcggg gcgactgtag aagagttctt aaaaaaattt
     2041 ccggatgcaa aaaagatcgg atcatcattc cctgtttttc ttgttgacgg tgcggaatat
     2101 gcttttgcaa ggatcgagaa aaaaagcggt ctgggacatc atggctttga aatagagtca
     2161 catcctgaag ttacactgat agaagacctg aaaagaaggg atctcacaat aaatgcaatg
     2221 gcagaagata ttgaaactgg tgaaattatt gatcctttcg gcggaatgaa agaccttgag
     2281 aacaaaagga tcgttcatgt gacagatgct tttgcagaag atccgctccg ggtttacaga
     2341 gttgcgaggt ttgcatcgcg gttctatgat ttctcaattg acaaaactac gatcaagtta
     2401 atgaattcat tgaagaaaga actgaacaca ctttctgttg aaagggtttg ggcggaatgt
     2461 cttaaagcac tttcatcaaa aaaaccggca agatttttta ctgttcttga tgaagcggga
     2521 gttctcgatg ttcattttcc ggagctttac aatttgaaaa atgttcctgc cggtccggtt
     2581 gaatatcatc cggacgatgc cgattctttt catcacacca ttagtgctct tgaaagggtg
     2641 tcggacattc aaagcggaac agatccgtta ctttcatttt ccgttatgtg tcacgatctt
     2701 ggaaaagctt taacaaatcc ggatgattat cctcatcatc acggacacga taagtcaggg
     2761 gttgaactga tcaaagcttt ctcaaaacgg ctgaaagtgc ccagaaaatt tgaagaagcg
     2821 gcagtgatat ttgcaaaaaa ccacatgaaa ataagcagaa tttttgaaat gacaccatca
     2881 aaagcactta aacttatt
//
LOCUS       DAOOYB010000333         2894 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_42199_length_2894_cov_9.688623, whole genome shotgun sequence.
ACCESSION   DAOOYB010000333 DAOOYB010000000
VERSION     DAOOYB010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42199_length_2894_cov_9.688623"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..2193
                     /locus_tag="PL056_13015"
     CDS             103..2193
                     /locus_tag="PL056_13015"
                     /inference="COORDINATES: protein motif:HMM:NF036933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgGFc-binding protein"
                     /protein_id="HPM47980.1"
                     /translation="MKVFTCLVFIVSIFFVSCSASEDETCSIDNPCPEGYTCNLMTGK
                     CEKEAVVDNEGSDDPVLPVDNEKDDKDVAEKTDNEKPDEIVIKECEPDELVMCDPDDF
                     YNILRCNSSGTGVDSVPCGEMTVCEDDKCVQQMCEPKTRVCNEEDLSKVFECNYTGTK
                     PSETVIEDCGVGGFCQAGECIFYCDIAANEHSYQGCEYFTAFLDTRTMRSDPVFAIVI
                     ANTNLEKVATISVTISSNGTTEDDAGKCVYCDDKISDTTCMLRTSAKDLFVQPGKIGI
                     IQLPHERMLRSTGKSWNSYHVKSNLPVTVYQFSPIDNSDNNPFNANGKSVDYSPIEIL
                     NNGKSFSNDASLLIPATSVYSNYMAVTYLSVLGSKADMTNEPWPSYITVIGVSENELE
                     LVITPEADIVAGGSVPAVTNGSDYEFSLKKYQVYNFETDENNKDLTGSRIRCKNPLEK
                     CGPFIVFSGNASTVIPNDKGYADHLEQQLFPIETWGKDYFLIKSSVKKDESDYARIIA
                     SEDNTTITYSPQSPTALAPYTSAAPATKLNAGEWTEFYFKDSYHIKSDKPVMIAHFLT
                     GADMISSECYNGTTEYHQANCPGDPALTLIPSSEQFRKDYIFLTPGSYKSNYATVVLK
                     NGTKAVLNEKELTSLTEITGTGFSYKIVDLGSDFARHSLLCDEPCGLYVYGWESDVSY
                     AYPGGLNLEKLSNN"
     gene            2197..2748
                     /locus_tag="PL056_13020"
     CDS             2197..2748
                     /locus_tag="PL056_13020"
                     /inference="COORDINATES: protein motif:HMM:NF025743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HPM47981.1"
                     /translation="MSELSAPVNANLTVSVMYKDDALLAETKSILESLYGNIDLVSDS
                     YSFSDISPYYDPEMGEGIRKIIFSFEKTVDRSILADVKHECVRIEKKYSENGSRTVNL
                     DPGLISLENFILATGKNYSHRIYLKDGVFAEVTLMFGKKTLIKELPWTYRDYLFEPAR
                     SFLLKVREGYRNKKEYLIKQSGE"
BASE COUNT          949 a          544 c          597 g          804 t
ORIGIN      
        1 ccttagaaat aataataatc ttacatttaa gttactatat tatttgaaaa agtgttcttt
       61 gcagttataa tctgatctgt tttctttatt tggaggatta ttatgaaagt ttttacttgt
      121 ctggttttta ttgtgtcgat tttttttgtt tcgtgttcag ccagtgaaga cgaaacatgc
      181 tccatcgaca atccctgtcc tgaaggttat acatgcaatc tcatgactgg aaaatgcgaa
      241 aaggaagcag ttgtagataa tgaaggatcc gatgaccccg ttcttcccgt tgacaatgaa
      301 aaagacgata aggatgttgc tgaaaagacc gacaatgaaa aacctgacga aatcgttatc
      361 aaagagtgtg agccggacga acttgtcatg tgtgacccgg atgattttta caacattctc
      421 cgctgcaaca gcagcggaac aggggttgat tcggttccat gcggggaaat gacagtctgt
      481 gaagacgata aatgcgttca gcagatgtgc gaaccgaaga caagagtgtg taacgaagaa
      541 gacctttcaa aagtttttga atgcaactat acaggaacaa aaccttccga aacagtaatt
      601 gaagactgcg gtgtcggagg attctgtcag gccggagaat gtatctttta ctgcgatatc
      661 gccgccaatg agcacagcta tcagggatgt gaatatttta cagcttttct tgacacaaga
      721 acaatgagat cggaccctgt ttttgcaatt gtaatagcaa atactaatct tgaaaaagtt
      781 gcaactataa gtgtaactat ttcatcaaac ggaacgactg aagatgatgc tggaaaatgt
      841 gtttactgtg atgataaaat cagtgacaca acctgtatgt taaggacttc cgcaaaagat
      901 ctttttgttc agcctggaaa aattggtatt attcaattac cacacgaacg gatgttgcgt
      961 tcaacaggaa aaagctggaa tagttatcat gttaaatcca accttcctgt aacagtatat
     1021 cagttcagtc ctattgacaa tagtgacaac aatcctttca atgcaaacgg aaaaagtgtt
     1081 gattacagcc ctattgaaat tttaaacaat ggcaaatcgt tttcaaacga cgcatctctt
     1141 cttattcctg ctacaagtgt atatagtaat tatatggcag taacatacct gtctgttctt
     1201 ggatcgaaag ccgacatgac taatgagccg tggccatctt atattactgt tatcggagta
     1261 agcgaaaatg aacttgagct cgttataact cctgaagcgg acattgttgc tggtggcagc
     1321 gttcccgctg taacaaatgg gagtgattat gaattcagtt tgaaaaaata tcaggtctat
     1381 aactttgaaa cagatgaaaa caataaggat ttgacgggaa gtcgaatcag atgcaaaaat
     1441 cctttggaaa aatgcggacc ttttatcgtg ttttccggca atgcatcaac agtaataccg
     1501 aatgacaaag gttatgcaga ccatcttgag cagcagcttt tcccaattga aacatggggc
     1561 aaagattatt tcttaataaa atcaagtgtt aagaaagatg aatctgatta cgccagaata
     1621 attgcatctg aagataatac aacaataaca tattcacctc aatctccgac cgctcttgct
     1681 ccctacacat cagccgctcc tgcaacgaaa ctgaatgccg gggaatggac agaattctat
     1741 tttaaagaca gttatcatat aaagtcagat aaacccgtga tgattgctca tttcttaacc
     1801 ggagcagata tgataagctc tgaatgttac aacggaacaa ccgaatatca tcaggcaaac
     1861 tgcccgggtg atccagcatt aaccctcatt ccgtcttctg agcagttcag aaaggactac
     1921 atattcctca cacccggaag ttataaaagc aattatgcaa cagtggtttt aaaaaatgga
     1981 acaaaagcgg tgttaaatga aaaagagttg accagtttga cagaaataac cggaacagga
     2041 ttttcataca aaattgttga cctcggaagt gattttgcaa gacattctct tttgtgcgac
     2101 gagccgtgcg gactttatgt ttacggatgg gaatcagatg tgagttacgc atatccgggc
     2161 ggactcaacc ttgaaaagct gagcaacaac tgatatatga gcgaactttc agctcctgta
     2221 aacgcaaatc ttacagtttc agtaatgtat aaagatgatg ctcttcttgc tgagacaaaa
     2281 agcatccttg aatcacttta cggcaatatc gatcttgtct ctgacagtta cagtttttct
     2341 gacatttcac cttattacga ccccgaaatg ggggaaggga taagaaagat cattttcagc
     2401 tttgaaaaaa cggttgacag gtcaattctt gccgatgtga agcatgaatg cgtcagaatt
     2461 gagaaaaaat acagtgaaaa cggttcaaga acggtcaatc tcgatcccgg gcttatttca
     2521 cttgaaaatt tcatccttgc gacaggaaag aactacagcc acagaatata cttaaaggat
     2581 ggcgtttttg ccgaagtcac gctgatgttc ggcaaaaaaa cactgataaa ggaactgccg
     2641 tggacatata gagattatct ttttgaacct gccagatctt ttcttctcaa ggttcgcgaa
     2701 ggatacagaa acaaaaaaga atatcttata aaacagagcg gagaataaat tgaatctgat
     2761 agtatttcct cttaaacatg aaaacccttt tcccgttcaa gttaagacgg gcgaaccgtt
     2821 attcattgac agcaacacta tgatactctg ttcgggaata ggaacagccg gtgtaaacaa
     2881 tctgggaaga gttc
//
LOCUS       DAOOYB010000334         2884 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_42415_length_2884_cov_6.176034, whole genome shotgun sequence.
ACCESSION   DAOOYB010000334 DAOOYB010000000
VERSION     DAOOYB010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42415_length_2884_cov_6.176034"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(201..1025)
                     /locus_tag="PL056_13025"
     CDS             complement(201..1025)
                     /locus_tag="PL056_13025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47982.1"
                     /translation="MSKKKKFFIASAVILSALIVTAIYEMRPERDLSEKLLPKEYQGR
                     DAITGLGDISSSRPVEKVAGDERVEGDEKVEIVEDVEKVEEKLPAGEKFKKANESISD
                     SFKKHGADAVLKPGDKLVEKEKYHGKYTLNEPDFSNLHPLNYQGLKKVYTYFRLKESM
                     PEVVVNKLNGAAVVMTGAVMPVDKIPEDGNFTAFWLSNPSIVLAGCVFCNPPTMADIV
                     YVYKDSGENPFKFEREKLFKQVVLVRVTGRLFFGPETIKDQIFLFSILATDVEILN"
     gene            complement(1027..2316)
                     /locus_tag="PL056_13030"
     CDS             complement(1027..2316)
                     /locus_tag="PL056_13030"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HPM47983.1"
                     /translation="MRAAFILKAVMHNMKHRCSAVVISTISIACAFAFLSAIGNVTFG
                     LATTAVNSSLSFPLVIGPEGSSDTQLVMSTVFNIDKPKGTLEYSVFEELIKDKRVASA
                     FPVARADSYMGIPITGVNSDFIKEKSNGFIKLKEGFVSDNIFPESDLHRAVIGFKIAE
                     RYGLTVDDKFAGSHGHVGDENAHRHDDFQYYVAGVMNPINGPEDFSIYVNLRAVWKIH
                     EKGDEHDDHNADNEHHEHHEGCDHGTDGEAHETLSAILVKTVNPVATSELEREYSSRS
                     GTSATDVAKTVRRVVQYMNKAETAAGFFSYGTLLIVLAMVFVTMVMAINERKKEMAMM
                     RTLGIGKLPISLTVMIESMIITVAGVLAGVVLGHFILWYLKPLIDFNLGISLEPFFVT
                     KVEIQGILVTLVSGQILSLVAMLRIYNMNLVEEVAKD"
     gene            complement(2313..>2884)
                     /locus_tag="PL056_13035"
     CDS             complement(2313..>2884)
                     /locus_tag="PL056_13035"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPM47984.1"
                     /translation="AVLRLKGASGSGKTTLMNIVSGLILPDSGTVKAGDFEITAMSET
                     GRDRFRAENIGYVFQTFNLLSPFSVIENVYAPLAFAGKLPEKFEEKAIAVLNEVGLAG
                     LEKRKIYHLSVGQRQRVCVARVLFSNPRIILADEPTASLDTESAKTVYRSIEKCRNNG
                     STIIFASHDRIFDDFNSDMVFDLETGRVS"
BASE COUNT          809 a          684 c          506 g          885 t
ORIGIN      
        1 aaatcttaaa tactttcaat gtctcctcca tttatgaata agtttctcaa accgtgtctc
       61 cttaaagcgc ctgccaaaat agtaaataca cctctaaaag tcaaatgttt aaatgaacat
      121 ttttcttaca cacacaattt agcaagacat cttccagact tttttattct ttaatatcaa
      181 caagttgcac agaagtctca tcaattcagt atctccacat cggttgcaag aatgctgaaa
      241 agaaatatct ggtcttttat tgtttccggg ccgaaaaata accgtccagt gactctcacc
      301 agaactacct gtttaaaaag cttttccctt tcaaatttaa agggattttc gccgctgtcc
      361 ttataaacat aaactatatc tgccattgtc ggtggattac agaaaacaca tcctgcaaga
      421 actatcgacg ggtttgaaag ccagaaggcc gtaaaattac catcttcggg aattttatca
      481 acaggcatta ccgctccggt cattacaacc gccgcaccgt taagcttgtt cacaactact
      541 tccggcattg attctttaag acggaaataa gtataaacct ttttaagtcc ctgatagttg
      601 agtggatgaa gatttgaaaa atcaggttcg ttaagggtat attttccgtg atatttttct
      661 ttttccacaa gtttgtcgcc gggttttagc accgcatcag caccgtgttt tttaaagctg
      721 tcagatatag attcatttgc ttttttgaac ttttcgcctg caggaagttt ttcctcaacc
      781 ttctcaacat cctcaacgat ctcaaccttc tcatcaccct caactctctc atcacccgca
      841 accttctcaa ccggtctgct tgaagaaata tcaccaagcc cggtgatggc atctctgccc
      901 tgatattctt tcggaagaag tttttcagaa aggtctcgtt cgggcctcat ttcatagata
      961 gctgtgacta tcaatgcaga taatatcaca gcggaagcga taaaaaattt cttctttttt
     1021 gacatatcag tccttggcta cctcttcgac aaggttcata ttgtagatcc tcagcattgc
     1081 aacaagtgag aggatctggc cggacaccag agtaaccaat attccctgta tttcaacttt
     1141 tgtgacaaaa aacggttcga gacttattcc gagattgaaa tctatcagcg gttttaaata
     1201 ccatagaata aaatgaccga gaacaacacc ggcaagaaca ccggcaactg taattatcat
     1261 tgattcgatc attacagtca atgaaatagg gagcttgcct attcccaaag ttctcatcat
     1321 ggccatctct ttctttcttt catttattgc catgaccatt gttacaaaaa ccattgcaag
     1381 aacgatcaat aatgttccgt aactgaaaaa tcctgcggct gtttcggctt tgttcatata
     1441 ctgcacaact cttctgactg tttttgccac atctgttgca cttgtaccgc ttctggagga
     1501 atattccctt tcaagttcac tcgtagcgac cggatttaca gttttcacaa gaatcgcaga
     1561 aagcgtttca tgggcttcgc catcagtacc gtgatcacac ccttcgtggt gttcatggtg
     1621 ttcattatct gcattgtgat cgtcgtgttc atctcccttc tcatgaattt tccagactgc
     1681 tcttaaattg acataaatag aaaaatcttc cggtccgttt ataggattca ttactcctgc
     1741 aacataatac tgaaaatcat cgtgacggtg agcattttca tcacccacat gtccgtgact
     1801 tccggcaaac ttgtcgtcaa ctgtgagtcc gtatctttcg gctattttaa aaccgataac
     1861 tgctctgtga aggtcagatt cagggaaaat gttgtcagaa acaaaacctt ctttcaactt
     1921 tatgaatccg ttgctttttt ccttaatgaa atcagagttt acccccgtta tgggaattcc
     1981 catgtaactg tcagcccttg caacaggaaa tgctgatgcg actcttttat ctttgatcaa
     2041 ttcttcaaaa actgaatatt caagtgtccc tttcggttta tcgatattaa aaaccgttga
     2101 catcacaagc tgggtatcgc ttgatccctc aggtccgata acaagcggaa agctcagaga
     2161 actgtttacg gccgttgtag ccagtccgaa agtcacattc ccgatagcac tcaaaaaagc
     2221 gaatgcacaa gctattgaga ttgttgatat taccacagcg gaacatctgt gtttcatatt
     2281 gtgcataact gcttttaaaa taaatgccgc cctcatgaca ccctccctgt ttcaagatcg
     2341 aataccatgt cgctgttaaa gtcatcaaat atcctgtcat gtgaggcgaa aattattgtc
     2401 gagccgttat ttctgcactt ttcgatcgat ctgtaaaccg tttttgcact ttctgtgtcc
     2461 aaagatgcgg tcggttcatc ggcaagaatg atccgggggt ttgaaaaaag aacccttgca
     2521 acacaaaccc tctgcctctg ccccaccgaa agatggtaga tcttcctttt ttcaagaccg
     2581 gcaagtccca cctcattcaa aacagcaatc gctttttctt caaatttttc agggagtttc
     2641 cctgcaaaag cgagaggtgc ataaacattt tcaatgacag aaaaaggact tagaagatta
     2701 aatgtctgaa aaacatagcc gatattctca gctctgaacc tgtctcttcc ggtttcgctc
     2761 attgcggtta tttcaaaatc acccgccttg accgttccgc tgtcaggaag aataagacct
     2821 gaaacaatgt tcatcagtgt ggtctttccg cttccgcttg cacccttcag gcgaagcacc
     2881 gctc
//
LOCUS       DAOOYB010000335         2876 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_42594_length_2876_cov_6.522864, whole genome shotgun sequence.
ACCESSION   DAOOYB010000335 DAOOYB010000000
VERSION     DAOOYB010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42594_length_2876_cov_6.522864"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1571)
                     /locus_tag="PL056_13040"
     CDS             complement(<1..1571)
                     /locus_tag="PL056_13040"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPM47985.1"
                     /translation="MSYVSLNWIYPFVYVVFGAAAVYLIYIITKYGVGLKASPREIWF
                     VSISQVTEFTAYSIMLMTLTLWLSSDVGLSDVAAGNYMGTWNLSYTILIIGVGSLVDA
                     VGVKKVLVIGTILAIFSRFFLFVSTDFWVVTILGFVPQAVSVAFLSPVISVALKRYTK
                     SDTSALGFAMFYTLMNIGFALGGLIFDWIRQIYGEYGNVMIPLLGEVSTYRFILFTAF
                     LISFPGMFFIAIMRDNIDLKDDGVLEILPKKEKKAGNMVVSIMKTIEESFANAAKIFV
                     DVAKQPAFWKFMALLAILLGVNYVFYHFHYTFPKYGIRVLGEGAKIGNIYGVLNPVII
                     VFFVPLIAWLTRKWSSYKMITIGSMISAASVFFVVLPDAFYAPIADTVAGRLIWQEWL
                     GVAEGTSVSTISVYLALCFFVIFFTVGEAIWSPRLMEYTAKIAPKGQEGSYLALSLLP
                     KFISQPLVGFMSGKLLEVYTPATEIVNEAGVKSQVVGDISNHYMIWIWVGAIAVISPI
                     GMIIFGKFLRGHEASS"
     gene            1723..>2876
                     /locus_tag="PL056_13045"
     CDS             1723..>2876
                     /locus_tag="PL056_13045"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HPM47986.1"
                     /translation="MKRLLVTMTCLLIAVVSCGDNDAMQKKMEETAERNKQIKEEMEA
                     KKKEKPKFVDETVKEEMPPIVEAATKGDLAAVKTELGKGTLVDTKDSRQRTALYQAVA
                     EGKKEVAKLLISEGANVNTKRDDGDTPLMAAARKGDLELAECLVNGGADVKATDNFGR
                     TALMMAASSGSKEVFDFVLKGSDINAADKYELTALIFAMKSGHMDLAKYILGKGAKVW
                     KPKKVTWSKKPIVLKIENKVQPGMDKVIEELDKKEVHLRQKTELFYAIEKGDLELVKL
                     LVAKGSPLLVTDVEQSRENIIYVYGPNKEEERDMLFERETVRTSYDRDQKNLLHYAAE
                     GGYVEIMKFLISKGARLEETDGENVRSPLFYAVGQDLDKKDPSKEKRLAET"
BASE COUNT          963 a          586 c          628 g          699 t
ORIGIN      
        1 gaacttgctt cgtggcctct taagaatttg ccgaatatta tcattccgat aggcgatatt
       61 actgcaattg cgccgaccca gatccagatc atgtagtgat ttgaaatgtc gcccaccacc
      121 tgagatttaa ctcccgcttc attcacaatc tcggttgccg gagtataaac ttcaagcagt
      181 tttcctgaca taaatccgac aagcggttga gatatgaatt taggaagaag tgaaagagcc
      241 agatagcttc cctcctgccc tttgggagcg atctttgcgg tatattccat cagccgcgga
      301 gaccagattg cctcacctac tgtgaagaat atgacaaaaa agcagagtgc cagatagacc
      361 gaaatagttg aaacgcttgt cccttcagca actccgagcc actcctgcca gatcaaccgc
      421 cctgcaacag tatcagcaat aggggcataa aaagcatcag gaagtacaac gaagaaaacc
      481 gatgcggcag atatcatcga gccgatagtt atcattttgt aggagctcca ttttcttgta
      541 agccatgcaa tgagcggaac aaaaaaaaca atgatcacag gatttaagac accgtagata
      601 ttacctattt tagcaccctc tccaagaact ctgatgccgt atttggggaa tgtatagtgg
      661 aaatgataga acacatagtt gacaccaaga agtatcgcta ataacgccat gaacttccag
      721 aatgccggct gttttgccac atccacaaat atttttgcgg catttgcaaa tgactcttct
      781 attgttttca tgatcgaaac gaccatgttt cccgcctttt tctctttttt aggaaggatc
      841 tcgagaacgc cgtcatcttt aagatcaata ttgtctctca ttatcgcaat aaagaacatg
      901 ccggggaaac ttatcaggaa cgcggtaaaa aggatgaacc ggtatgttga aacctctccg
      961 agaagcggga tcattacatt tccgtactct ccgtatatct gccttatcca gtcaaatatc
     1021 agtccgccga gagcgaaacc gatgttcatc agagtataga acattgcaaa tccgagagcg
     1081 gaagtgtctg attttgtata tcttttcagt gcaactgata ttacaggact taagaatgca
     1141 acactcactg cctgaggaac aaagccgaga atggtaacaa cccagaaatc agtcgaaaca
     1201 aaaagaaaga acctcgaaaa tattgcaagg atagttccta tcaccagcac ttttttcaca
     1261 cccacggcat caacaagtga ccctacaccg attatgagta tggtatatga aagattccat
     1321 gttcccatgt aattacctgc cgcgacatcg ctaagtccga catcgctgga aagccacagt
     1381 gtcagagtca tgagcataat ggaataagca gtaaattcag taacttgcga gatcgaaaca
     1441 aaccagatct ctctgggaga tgccttaagt ccgacaccat attttgtaat gatgtagatc
     1501 agataaacag ccgccgcacc gaaaacgaca taaacgaaag gatagatcca gttgagtgaa
     1561 acatacgaca tgaaaacctc ctcataaaag tctgagcaaa ttagcagaag tattgaaaaa
     1621 tgtcaaaatc ttaaaaaaaa ttgataaaaa tattactatt atgtataagt ttgcagttat
     1681 gttggaaatc gtgcttattt tttcgagatg gagactaaaa aaatgaagag attactcgtt
     1741 actatgactt gtcttcttat cgctgttgtt tcctgcggtg acaacgatgc aatgcagaag
     1801 aaaatggaag agaccgctga aaggaacaaa cagatcaagg aagagatgga agcaaagaaa
     1861 aaggaaaaac ccaagtttgt tgatgaaacc gttaaagagg agatgccgcc tattgttgaa
     1921 gccgcgacaa aaggagatct cgccgcagtt aaaactgaac tcggcaaggg aactcttgtg
     1981 gacacaaagg acagcagaca gagaacagct ctttatcagg cagttgctga aggtaaaaag
     2041 gaagttgcaa aacttctcat ttccgaaggg gctaatgtca acacaaaaag ggatgacgga
     2101 gatacacctc tcatggccgc agcaagaaaa ggagatctgg aacttgcaga gtgtcttgtc
     2161 aatggcgggg cagatgtaaa ggcaactgac aatttcggaa gaaccgcact tatgatggcg
     2221 gcatcaagcg gatcaaaaga ggtttttgac tttgttctca aaggatcaga tatcaatgcg
     2281 gctgacaaat atgaacttac agcgctcatt tttgcaatga agtcaggtca catggatctt
     2341 gcaaaatata ttctcggcaa aggtgcaaaa gtatggaaac cgaaaaaggt cacatggagt
     2401 aaaaagccga tcgttctaaa aattgagaac aaggttcagc ccggaatgga taaggtcatt
     2461 gaagaacttg ataaaaaaga ggttcatctg cgccagaaaa ccgaactttt ctatgcgata
     2521 gaaaaaggtg atcttgaact tgtgaaactt cttgttgcaa agggctcgcc ccttcttgtt
     2581 accgatgttg agcagagtcg tgagaacata atttatgttt acggaccgaa caaggaagaa
     2641 gaaagagaca tgctttttga aagagaaact gtcagaacaa gttatgaccg tgatcagaaa
     2701 aaccttcttc attatgctgc tgaaggcggt tatgttgaaa taatgaaatt cctcatctca
     2761 aaaggtgcgc ggctcgaaga gaccgatggt gaaaatgtaa gaagtcctct tttctatgct
     2821 gtaggacagg accttgacaa gaaagatcct tcaaaagaga aacggcttgc tgaaac
//
LOCUS       DAOOYB010000336         2876 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_42595_length_2876_cov_6.253456, whole genome shotgun sequence.
ACCESSION   DAOOYB010000336 DAOOYB010000000
VERSION     DAOOYB010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42595_length_2876_cov_6.253456"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(202..1008)
                     /locus_tag="PL056_13050"
     CDS             complement(202..1008)
                     /locus_tag="PL056_13050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47987.1"
                     /translation="MSKKKKFFIASAVLFSVLLVTAIYEMRPEKDLSEKLLPKEYQGK
                     DAIGALGDISESRPVESVEKAESVEKAESVEKAEKKLPAEEKFKKANESISDSFKKHG
                     ADVVLKPGDKLVEKEKYHGKYTLNEPDFSKLHPLNYQGLKKVYTYFRLKESMPEVVVN
                     KLNGAAVVMTGAVMPVDKIPEDGNFTAFWLSNPSIVLAGCVFCNPPTLADIVYAYKDR
                     GENPFKFEREKLFKQVVLVRVTGRLFFGPETIKDQTFLFSILVTDIEVLN"
     gene            complement(1010..2308)
                     /locus_tag="PL056_13055"
     CDS             complement(1010..2308)
                     /locus_tag="PL056_13055"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPM47988.1"
                     /translation="MRAAFILKAVLHNMRHRCAAVTVSIISIACAFAFLSAIGNVTFG
                     LAATAVNSSLSFPLVIGPEGSSDTQLVMSTVFNIDKPKGTLEYSVFEELIKDKRVASA
                     FPVARADSYMGIPITGVNSDFIKEKSNGFIKLKEGFVSDNIFPESDLHRAVIGFKIAE
                     RYGLTVDDKFAGSHGHVGDENAHGHDDFQYYVAGIMNPVNGPEDFSIYVNFKAVWKIH
                     EHHGEHTEHKHEAEHEAEHEHHEGCEHSNEGSSHETLSAILVKTVNPVATSELEREYS
                     SRSGTSATDVGKTVRKLVQYMNKAETAAGFFSYGTLLIVLAMVFVTMVMAINERKKEM
                     ALMRTLGIGKFPISLTIMLESMIITVAGVVAGVAAGHFVLWYFKPLIDFNFGISLEPF
                     FVTKVEIQGILVTLVSGQILSLVAMLRIYNMNLVEEVARD"
     gene            complement(2305..>2876)
                     /locus_tag="PL056_13060"
     CDS             complement(2305..>2876)
                     /locus_tag="PL056_13060"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPM47989.1"
                     /translation="AVLRLKGASGSGKTTLMNIVSGLILPDSGKVKAGDFEITAMSET
                     GRDRFRAENTGYIFQTFNLLSPFSVIENIYAPLAFAGKLPEKYKEKAISVLNDVGLSG
                     LEDRRIYHLSVGQRQRVCVARVLFMEPKIILADEPTASLDAESAKTVYRSIEKCRSNG
                     STVIFASHDRIFDDFSSDLIFDLETGRIS"
BASE COUNT          830 a          670 c          489 g          887 t
ORIGIN      
        1 aaatcttaaa tactttcaat gtctcctcca tttatgaata agtttctcaa accgttctcc
       61 tttgaaacgc cagccaaaat agtaaatacc ccttaaaaag tcaaacttta aatgaacctt
      121 ttttcacaca cacactatta agcaaaataa aatccaaact tttttaacca atatattcaa
      181 atcattatct ctcaaataca atcaattcaa cacttcaata tcggttacaa gaatactgaa
      241 aagaaatgtc tgatccttaa ttgtctcagg accaaaaaat aaccgtccgg tgactcttac
      301 cagaactacc tgcttaaaaa gcttctctct ttcaaatttg aaaggatttt cacctctatc
      361 tttataagca tagactatgt cagcaagtgt gggcggattg cagaaaacac atcctgcaag
      421 aactatcgac gggtttgaaa gccagaaagc tgtaaaatta ccatcttcag ggattttatc
      481 gacaggcata acagctccgg tcattacgac cgccgccccg ttaagtttgt tcacaactac
      541 ttccggcatt gattctttaa gacggaaata ggtataaacc tttttaagtc cctgatagtt
      601 aaggggatga agcttagaaa aatcgggttc attcagagta tattttccgt gatatttctc
      661 tttttccaca agtttgtccc cgggtttgag aaccacatca gctccgtgct tcttaaagct
      721 gtcggaaatg gattcgttcg cttttttgaa tttttcctca gccggaagtt ttttctccgc
      781 tttctcaaca ctctccgctt tctcaacact ctccgccttc tcaacactct caaccggcct
      841 gctttcagaa atatctccta aagccccgat tgcatctttt ccctgatatt ctttcggaag
      901 aagcttttca gaaaggtctt tttccggcct catttcatag atagccgtta ccagcaatac
      961 agaaaataaa actgcggagg caataaaaaa cttcttcttt tttgacatat cagtccctgg
     1021 ctacctcttc gacaaggttc atattgtaga tcctcagcat tgcaacaagt gagaggatct
     1081 ggccggacac cagagtaacc aatattccct gtatttcaac ttttgtgaca aaaaacggtt
     1141 cgagacttat tccgaaattg aaatcaataa gaggtttaaa ataccataaa acaaaatgac
     1201 cggcagcaac tccggcaaca acaccagcaa ctgtaattat cattgactca agcattatag
     1261 tcagtgaaat agggaacttg ccaattccca gcgtcctcat cagagccatc tctttttttc
     1321 tttcattaat tgccatgacc attgttacaa aaaccattgc aagaacgatc agaagggttc
     1381 cgtaactgaa aaatcccgca gcggtttcgg ctttgttcat atactgaact aattttctga
     1441 cagttttccc gacatcagtt gcacttgttc cgcttctgga tgaatattcc ctttcaagct
     1501 cgctcgttgc aaccgggttt accgttttaa caagaattgc ggaaagcgtt tcatgggatg
     1561 atccttcatt actgtgttca catccttcat ggtgttcgtg ttctgcttcg tgttctgctt
     1621 cgtgtttgtg ttctgtgtgt tcaccatgat gttcgtgaat tttccataca gctttaaaat
     1681 tgacataaat cgaaaaatct tcaggcccgt tcacaggatt cattattcct gcaacataat
     1741 attgaaaatc atcatgaccg tgagcatttt catcacctac atgtccgtga cttccggcaa
     1801 acttgtcgtc aactgtgagt ccgtatcttt cggctatttt aaaaccgata actgctctgt
     1861 gaaggtcaga ttcagggaaa atgttgtcag aaacaaaacc ttctttcaac tttatgaatc
     1921 cgttgctttt ttccttaatg aaatcagagt ttacccccgt tatgggaatt cccatgtaac
     1981 tgtcagccct tgcaacagga aatgctgatg cgactctttt atctttgatc aattcttcaa
     2041 aaactgaata ttcaagtgtc cctttcggtt tatcgatatt aaaaactgtt gacattacaa
     2101 gctgtgtgtc gcttgatccc tcaggaccga taacaagcgg aaaactcaga gaactgttta
     2161 cagccgttgc agcaagtccg aaagtcacat ttccgatagc actcaaaaaa gcgaatgcac
     2221 aagctattga aattattgat acagtaactg ccgcacacct gtgtctcatg ttgtgcagca
     2281 ccgcttttaa aataaatgcc gctctcatga tatcctccct gtttcaagat caaatatcag
     2341 gtcgctgcta aagtcatcaa atatcctgtc atgcgaggcg aaaattactg tcgagccgtt
     2401 acttctgcac ttttcgatgg atctgtaaac cgtttttgca ctttccgcat caagtgatgc
     2461 tgtgggttca tcggcaagaa ttatcttggg ttccatgaaa agaactcttg caacacatac
     2521 tctctgtctc tgacctacgg aaagatgata tatccttcgg tcttcaagac ctgaaagtcc
     2581 gacatcattc aacacagaaa tggctttttc tttatatttt tcaggcagtt tacctgcaaa
     2641 agcgagaggt gcataaatat tttcaataac agaaaatgga cttaaaagat tgaatgtctg
     2701 gaaaatataa ccggtatttt cagctctgaa cctatctctt ccggtttcgc tcattgcggt
     2761 tatttcaaaa tcacccgcct taacctttcc gctgtcagga agaataagac ctgaaacaat
     2821 attcatcagt gtggtctttc cgcttccgct tgcacccttc aggcgaagca ccgctc
//
LOCUS       DAOOYB010000337         2870 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_42714_length_2870_cov_9.842629, whole genome shotgun sequence.
ACCESSION   DAOOYB010000337 DAOOYB010000000
VERSION     DAOOYB010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42714_length_2870_cov_9.842629"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            88..981
                     /locus_tag="PL056_13065"
     CDS             88..981
                     /locus_tag="PL056_13065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47990.1"
                     /translation="MKFRKFSAFLMILAIFNSSLHASIFGEENATLTAILTEEIIQVT
                     QIAETISQLKAIVDAGNQTINLAREGYRQFQKIKNYTWQELLSDAKAGFCKGLDSTTK
                     TSCSDWEASLNEMKDNYDMIKGGEGQQWVGYRSQWDNQTRSFLKQMYHGSAKAYVYPK
                     IAPNVSKFYGWDKHENDAEYVINQALLKSGLYKSVIETQKEGYVVQSAIGDFMREAEE
                     SKNLVAQGQATQMMQDQQRNKILTEENNRDKARFLHEENTKTQIRQERKVFMDGYREG
                     SKKSSSGKVWNTTVAEPPRED"
     gene            971..1507
                     /locus_tag="PL056_13070"
     CDS             971..1507
                     /locus_tag="PL056_13070"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HPM47991.1"
                     /translation="MKIKVYICIFIILIIFPLFLFPVEPCSLTAVIERSVKTYPYYVN
                     NKKYFIHPALILGIIEQESSFDPEAKSHAGAYGLTQIMPKTAKILKCDYDKLNNPELA
                     INCSVKFLAALLTYNKGDLIKSLSGYNGGTHSTETKATTADGLLAGRIYDHPETKKYV
                     VSVLKRFENFKNLNCKKQ"
     gene            1578..2735
                     /locus_tag="PL056_13075"
     CDS             1578..2735
                     /locus_tag="PL056_13075"
                     /inference="COORDINATES: protein motif:HMM:NF014273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HPM47992.1"
                     /translation="MATPKTNEKKLMKISIEKLEAMNYRDVAGDIDDIRDSLLEKGQL
                     EPITVTPKIEEGKGTDRFIIVNGERRYRAAKNIVESGRETKFNFNELDCIIDTSYNLD
                     TDSENFKLNQLILNEVHKSTTFETYLAVKSLVDSKKYSKSQISKALGKDETWAGKIAA
                     LIIDNELFKCYFSGADLIYNKNNAETFVSLNSFVDSKKDLITEKEKGRVAAFLKSKTV
                     ELAEWEKENGPLFETAELKPYAVYKGFGADSSPSIWGAQSLVEFYNDYCIDHPKKMEA
                     ELLFSRMIRALMLKRRKTKADIDRLVEGARAKLTDDRELEKEQKTTEITPKDMAKRIF
                     NLFKDIQFTDEIVRETKKEFRHLIKDAKHRDRLNIDISFAIRQKKEDETEE"
BASE COUNT         1018 a          530 c          626 g          696 t
ORIGIN      
        1 ggccagagtg ttactttgcg gtatataggc ataaatcaga gtgtttcaga tgatgcaatg
       61 agagaattta atttatagag gttttttatg aaattcagaa aattttcagc atttttaatg
      121 atcctcgcta ttttcaacag ctctcttcat gcctcaattt tcggggagga gaacgctaca
      181 cttaccgcaa ttctcactga agagataata caggtcactc agatcgccga aacaatatca
      241 cagcttaaag caattgtcga tgccgggaac cagaccataa atcttgcaag ggaaggatac
      301 cgccagttcc agaaaataaa gaattacaca tggcaggaac ttctttccga tgcaaaggcg
      361 ggattctgta agggactgga cagcaccact aaaacaagtt gttccgactg ggaggcaagt
      421 cttaatgaaa tgaaggataa ttatgatatg atcaaaggcg gagaaggtca gcagtgggta
      481 ggataccggt cacagtggga caatcagacc cgttcattct taaagcagat gtatcacgga
      541 tctgcaaagg cctatgttta tccgaagatc gcccccaatg tgtcaaaatt ctacggatgg
      601 gacaaacacg agaacgatgc cgaatatgtg ataaatcagg cacttcttaa atcagggctt
      661 tacaaatcag ttatagagac acaaaaggaa ggatatgtgg tacagagtgc gataggggac
      721 tttatgcgtg aagccgagga atctaaaaac cttgttgcac agggacaggc gacacagatg
      781 atgcaggatc agcagagaaa caaaatactt accgaagaga ataaccgtga caaggcgaga
      841 tttcttcacg aggaaaacac taagactcag ataaggcagg aaagaaaggt tttcatggac
      901 ggctacaggg aaggatctaa aaagtcctct tccgggaaag tatggaacac cacagttgcg
      961 gaaccgcccc gtgaagatta aagtttatat ttgtattttt ataatattaa ttatatttcc
     1021 tttatttcta tttccggtcg aaccgtgttc actcacggct gtaattgaaa gatcggttaa
     1081 aacctatcct tattatgtaa acaataaaaa atattttatc catcccgccc tgatactcgg
     1141 aattattgaa caggaaagca gcttcgaccc cgaagctaaa agtcacgccg gagcttacgg
     1201 actgacacag ataatgccta agacggcaaa gatactgaaa tgtgactatg ataaactgaa
     1261 taatcccgag cttgcgataa actgttcagt caaattcctt gcggcacttc tcacatacaa
     1321 taaaggcgac cttataaagt ccttatccgg ttacaacggc ggaacccatt caaccgaaac
     1381 aaaggcgact acagcagacg gccttttagc gggcagaata tacgatcatc ctgaaacaaa
     1441 aaagtatgtt gtttcagtcc ttaagagatt tgagaatttt aaaaatctaa attgtaaaaa
     1501 acagtaaaaa aatctttaca tatatatctg ttttcttaat atgaatatgt tctatctttt
     1561 aaacggagga tttttaaatg gctacaccga aaactaatga aaaaaagtta atgaagatct
     1621 caattgaaaa acttgaggca atgaattaca gggatgttgc cggggacatt gacgacatcc
     1681 gggacagtct gcttgaaaag ggacagcttg aaccgataac agtgaccccg aagatcgagg
     1741 aagggaaggg gacagaccgc tttattatcg tgaacggtga aagaaggtat agggcggcaa
     1801 agaacatcgt tgaaagcgga agagagacaa agttcaattt caatgaactg gactgcatta
     1861 ttgacacaag ctacaatctt gatactgatt ccgaaaattt taaacttaat cagctcatat
     1921 tgaacgaagt ccataaatct actacctttg aaacatacct tgccgtgaag tcgcttgtcg
     1981 attcaaaaaa atacagcaag tcgcagatct caaaggcact tggaaaagac gaaacatggg
     2041 cgggcaaaat agcggcactc ataattgata acgaactttt taaatgttat ttttcaggtg
     2101 cggatctgat ctataataaa aacaacgcag agacatttgt ttcactgaat tcttttgtgg
     2161 attcaaagaa agacctcata actgaaaaag agaaggggag ggtggcggca tttctgaaat
     2221 caaagaccgt tgaacttgcg gaatgggaaa aagagaacgg acctcttttt gaaaccgccg
     2281 aacttaaacc ttatgcagta tacaaaggat tcggagccga ctcgtcaccg tcgatatggg
     2341 gagctcaatc actcgttgaa ttttacaatg actactgtat tgaccacccg aagaaaatgg
     2401 aagccgaact tcttttttca agaatgataa gagcgttgat gctcaagaga agaaagacaa
     2461 aagccgacat cgacagactt gtcgaaggag cacgggcaaa actgacagac gacagggaac
     2521 ttgagaagga acagaagact acagaaatta cacctaaaga catggcaaaa agaatattca
     2581 atctttttaa agacattcag tttacagatg aaatagtcag agagacaaag aaggaattca
     2641 ggcatcttat taaagatgca aagcacagag acagactcaa tattgacata tcatttgcaa
     2701 tcagacagaa gaaggaagat gaaacagaag aatagcggtt gtcgggaatt tttcttgttc
     2761 caattggaaa tgttttcaat aggaacaaag agtgaatttc taaaagtggt aaaaaacaag
     2821 aagattccga accttacggt ttcgggtctc cgacatgaaa aagcaatccg
//
LOCUS       DAOOYB010000338         2863 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_42855_length_2863_cov_5.410969, whole genome shotgun sequence.
ACCESSION   DAOOYB010000338 DAOOYB010000000
VERSION     DAOOYB010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42855_length_2863_cov_5.410969"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1593
                     /locus_tag="PL056_13080"
     CDS             <1..1593
                     /locus_tag="PL056_13080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47993.1"
                     /translation="TALFVFMLFTRNFGYAFQWRHLKKHLIGFGVYIAVIAPWMGYMW
                     ISEGFEFIKIFIWFHHFKRVAGEIHKPNDLYTLYLRILGYAMFPWSAFIPAAIYHFMS
                     RSRELISNNKKIFLMSMAVGPFFFLSFSGTKFYHYIAPVVPVIAIFLGVYFDKLWTAR
                     WELSSKIEAVIAIMLVAAIGRDIGNKYGLWLHIVTFYNERSLDRIPSWLFVIIPVFSA
                     FAITLFSMIFSSFMRKKGFYFLFVLAAGFMTYYFAVCLPVISNAYSLKPHVDAYIKDS
                     PERAPIADYYKWLRKSVGFNLRNDVTFLQADKEKNVLRFFDKPGTQYVILRPNDKKRF
                     ESLMKRINKSTEPVFKDSRNQLLKVSGKGKERDFSKSKQYRVDNVPGDIVRSDVIFDE
                     VIKLEGYKIVSGKIFHKEGRTWSKENNTITIDLYFKALADSITKDYDVFLHTEGNQKD
                     KRTKGDENMAMGTYPTTFWKKGDIVRHPIKVRIPGGSKNEYYIPYVGIYQEEYRGNIT
                     NRDDVPNDGDNRYELLKIYLDK"
     gene            1595..2788
                     /locus_tag="PL056_13085"
     CDS             1595..2788
                     /locus_tag="PL056_13085"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HPM47994.1"
                     /translation="MAELLAPAGNPEKLLWATVYGADAVYFGVDRFSLRSFAGNFTVD
                     DAQIGLEYLHKNGKKGYVTLNIYPYSGEYGDLIRTAHELEDCKADAFIVSDIGVINEL
                     KKAGIKTDIHISTQANTVSYQAVDAYFEMGAHRVNLARELSFEKIVEITKKKNPSVEV
                     EVFIHGAVCFSFSGRCAISDWLTGRKANRGECTHPCRWNYAVVEEKRPGVFMNVGEDE
                     RGTYLFNPKELALFEYMKPLSDAGVSSFKIEGRMKSIHYIASTVSLYKRLLNGENIPK
                     DEVFKLLNRIKNRGYSTGFMKGNVTPDDYQREGDGSNSEADFVANITDEKKDGDFVVE
                     VRNRLFAGETLEVLEPSGKTGEIKMPEIFHFTDGTSDNVAHHSKKILLKGDFTPYTIF
                     RRIDK"
BASE COUNT          906 a          493 c          634 g          830 t
ORIGIN      
        1 acagctcttt ttgttttcat gctttttaca agaaatttcg gatatgcgtt tcaatggcgg
       61 cacttaaaaa aacatcttat aggattcggt gtctacattg ccgtcattgc accatggatg
      121 gggtatatgt ggattagtga aggttttgaa tttattaaaa ttttcatatg gttccatcat
      181 tttaagagag ttgcgggaga gatacacaaa ccgaatgatc tttacactct ttatttaagg
      241 atcctcggat atgcgatgtt cccgtggagt gcttttatcc ctgcggcgat atatcacttc
      301 atgtcaagaa gcagagaatt gatatccaac aataaaaaga tctttcttat gagcatggca
      361 gtcggtccat ttttctttct ctcattctca ggtacaaaat tttatcatta tattgctccc
      421 gtagttccgg taattgctat atttcttgga gtttatttcg acaagctttg gactgcaaga
      481 tgggaactgt cctcgaagat agaagctgta atcgccataa tgcttgtcgc ggccataggc
      541 agagatatcg gcaataagta cggtttgtgg ctgcacattg ttacatttta caacgaaaga
      601 tcccttgaca ggatcccgag ctggcttttt gtaataattc ctgttttctc agcttttgcc
      661 ataactcttt tctcaatgat tttcagttct tttatgagaa agaaaggatt ttattttctg
      721 tttgtgctgg ctgccggatt catgacatat tattttgcag tgtgtctgcc tgttatatca
      781 aatgcgtact cattaaaacc gcatgttgat gcctatataa aagattctcc cgaaagggct
      841 ccgatagctg attactacaa atggcttaga aaatctgtcg gtttcaactt gagaaatgat
      901 gtcacttttc tccaggcgga taaggaaaag aatgtcctga gattttttga taaaccggga
      961 acccagtatg tcattctgcg gccgaatgat aagaaaaggt ttgaatcatt gatgaaaagg
     1021 atcaataaaa gtaccgaacc tgttttcaag gattccagaa accagctctt gaaagtaagc
     1081 ggcaaaggaa aagagaggga tttttcaaag tcaaagcagt atagagtcga caatgtgcca
     1141 ggtgatatag tcagatcaga tgtcattttt gatgaagtga taaagcttga aggatataaa
     1201 attgtgagcg gtaaaatatt tcataaagaa ggcagaacat ggtcaaaaga gaataacacg
     1261 ataacaatag atctctattt caaagcactt gccgacagta tcaccaagga ttatgatgtt
     1321 tttctccata cggaagggaa tcaaaaagat aagcgcacga aaggtgatga gaatatggca
     1381 atggggacat atcccacaac attctggaaa aaaggagata tagtgcgtca tcctataaag
     1441 gtcaggatcc cgggaggatc aaagaatgaa tattacattc cttatgtcgg aatttatcag
     1501 gaagaatatc gcggcaacat aactaacaga gatgatgttc caaatgacgg tgacaaccgt
     1561 tacgaactgc tgaaaattta tctggataaa taaaatggcg gaacttcttg ctcctgccgg
     1621 aaaccccgaa aagctgctct gggcgacagt ttatggtgca gatgcggtat atttcggagt
     1681 tgaccggttt tctttaaggt cttttgcagg caattttact gtagacgatg cacaaattgg
     1741 gcttgaatat ctccataaga atggaaaaaa aggatatgtc acgcttaaca tttatcctta
     1801 ttccggtgag tatggagatc ttataagaac ggcacacgaa ctcgaggatt gcaaagcgga
     1861 tgctttcatc gtaagtgaca tcggtgtcat taatgaactt aaaaaagcag gtataaagac
     1921 agacattcac atatcaacgc aggcaaatac tgtttcgtat caggcggttg acgcatattt
     1981 tgaaatgggg gcacacagag ttaaccttgc aagagagttg tcatttgaga aaatagttga
     2041 gataactaag aaaaaaaatc catctgttga agtcgaagtc ttcattcacg gtgcagtctg
     2101 cttcagtttt tcaggaagat gtgcaataag cgactggctt acaggaagaa aggcaaaccg
     2161 cggagaatgt actcatccct gcagatggaa ctatgcggtt gttgaagaaa agcgccccgg
     2221 cgtttttatg aatgtcggag aagatgaaag ggggacttat cttttcaatc cgaaagagct
     2281 ggcgcttttt gaatatatga agccgctgtc agatgcagga gtaagttcat tcaaaattga
     2341 aggcagaatg aaatcgatac actacattgc gtcgacagtt tcgctttata aaaggttgtt
     2401 aaatggtgag aatattccaa aagatgaagt tttcaagctt cttaacagaa tcaagaaccg
     2461 tggttacagc accggtttta tgaaaggaaa tgttactccc gatgactatc agagggaagg
     2521 tgacggatca aattctgaag ctgattttgt cgcaaatatc actgatgaaa aaaaggatgg
     2581 cgactttgtg gttgaagtca gaaaccggct ttttgcaggg gaaacccttg aagttcttga
     2641 gccgtcagga aaaaccggtg aaataaagat gcctgaaatt tttcatttta ctgacggaac
     2701 aagtgataat gtcgcccatc attcaaagaa aattcttcta aaaggagatt ttactcctta
     2761 cacaattttc aggagaattg ataaatgagt tggcacagga tcgtccgaat ccctgcaaaa
     2821 aagaacattt ttttccattt tacgattgaa agttatgata ata
//
LOCUS       DAOOYB010000339         2842 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_43306_length_2842_cov_7.209186, whole genome shotgun sequence.
ACCESSION   DAOOYB010000339 DAOOYB010000000
VERSION     DAOOYB010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43306_length_2842_cov_7.209186"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..685)
                     /gene="thiF"
                     /locus_tag="PL056_13090"
     CDS             complement(89..685)
                     /gene="thiF"
                     /locus_tag="PL056_13090"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS adenylyltransferase
                     ThiF"
                     /protein_id="HPM47995.1"
                     /translation="MIFDRNIPGTSEILQKKCVAVAGCGGLGSNIAIALVRSGVGNLI
                     IADFDKVETSNLNRQQYLFEDIGKLKASTLATHLKKINPEVSVKVVEKKLEPSDIKDQ
                     FSQADIMIEAFDAASSKLWLIESWTEAFPERSIVVGSGMSGLGKFEDIHVRIAGNLYI
                     CGDEQSEMTQGLMAPRVAIVANLQAMTAIEILLRKNNL"
     gene            complement(685..1257)
                     /locus_tag="PL056_13095"
     CDS             complement(685..1257)
                     /locus_tag="PL056_13095"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPM47996.1"
                     /translation="MKEKFRNVFSSILNRMEDKVLADKVIQVWVDCAVEGGWKSVEEI
                     AEIPFTLLTETEGVNLIEHTIAVTKGALGIADAMISTYRKVPFDINIDWVIAGGILHD
                     VGKLMEIEKVDGKYRKSYAGKCARHPISGAIAAAKAGLPMEIQNIIICHAKEGEGRPQ
                     RIEGVFIHQADFATFDPCVMKQKGLLIEKK"
     gene            complement(1291..1449)
                     /locus_tag="PL056_13100"
     CDS             complement(1291..1449)
                     /locus_tag="PL056_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004372607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HPM47997.1"
                     /translation="MKKYVCDVCGYVYDPAVGDPDGNIAPGVAFEDLPEDWLCPVCGV
                     GKDSFSEE"
     gene            complement(1457..2668)
                     /locus_tag="PL056_13105"
     CDS             complement(1457..2668)
                     /locus_tag="PL056_13105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HPM47998.1"
                     /translation="MSSNFKAVKVTDSVYWVGAVDWGVRYFHGYSTKSGTTYNAFLIM
                     ADKITLIDGVKHGFEKELLERVASVVDPQRIDYIISNHSEPDHSGTIPEIIKAVKPQK
                     VFASKNGVKALKSHFGDMGVEITAVDDGGELSLGNRTLKFMETRMLHWPDSMFTYLVE
                     EEVLFSQDAFGMHYASSERFDDEIPWTKLNEMAAEYYANIITLYSSFVQGLFKKVQTS
                     GLSFKIVAPDHGPVWRNMDNFSKVLGLYQRWSSRTTSRKAVIVYDTMWHATEKMARYI
                     EDGLLSTGVSVKVLPLQSASRSDVATEMLEATAVIVGTPTLNNNLFPSVADALTYLKG
                     LKFQTPFGAAFGSYGWSGEGVSQIKQYLTDMGCEIAGAVNAKYQPDEKVVAECFNLGV
                     EIGNKIINKIK"
BASE COUNT          744 a          701 c          509 g          888 t
ORIGIN      
        1 ttttctatca ttttttgttc caacaagttt ctcttcagtt tatataagat tgtttttgat
       61 ggcaagaaaa gttttagttg aaacagactt acagattgtt ttttctgagc aatatctcta
      121 tcgctgtcat tgcctgaaga tttgcgacaa ttgcgactct tggtgccatt aaaccctgtg
      181 tcatttcgct ttgttcatct ccgcagatat agagattgcc tgcaattctg acatggatgt
      241 cttcaaattt tccaagtcct gacattccgc ttccaaccac gatcgatctt tcagggaatg
      301 cttcagtcca cgattcgatg agccagagct ttgaagaagc ggcatcaaat gcctctatca
      361 ttatatcagc ttgtgagaat tgatctttaa tatcagatgg ttccagtttt ttttcaacaa
      421 ctttgacact tacttcagga ttgatctttt taaggtgtgt cgcaagagtt gacgctttca
      481 gttttccgat atcttcaaaa agatactgct gtctattgag attgctcgtt tcgaccttgt
      541 caaaatcggc aattatcaga tttccgactc cgcttcttac aagtgcaatg gcaatatttg
      601 aaccgagacc accgcaacct gcaactgcaa cacatttctt ttgaagaatt tctgatgttc
      661 cgggaatatt cctgtcgaat atcattattt tttctcgata agaagtcctt tctgcttcat
      721 tacgcacgga tcgaatgttg caaaatcggc ctgatgaatg aaaacgcctt caattctctg
      781 cggtctcccc tcgccttctt ttgcatgaca tatgataatg ttctggatct ccatcggaag
      841 acctgctttt gccgctgcaa tcgcacctga tatcgggtgt cttgcacatt ttcctgcata
      901 actttttctg tatttgccgt caactttctc aatttccatc aacttgccga catcgtgcag
      961 aattccacct gcgatcaccc agtcaatatt gatgtcaaaa ggaacctttc tatatgtcga
     1021 aatcatcgca tctgcaattc cgagagcccc ttttgtcacg gctatcgtgt gttcgatcag
     1081 attcacacct tctgtttctg tcagaagagt gaatgggatt tcagcgatct cttcaacact
     1141 tttccagccg ccttcaacag cacagtcaac ccaaacctga ataaccttgt cagcaagtac
     1201 cttgtcttcc attctgttta gaatgcttga aaacacattg cggaactttt ctttcatatt
     1261 tttctccaca atttaagata ttttaaggaa ctactcttca gaaaaagaat ccttgcctac
     1321 tccgcatacc gggcagagcc agtcttcagg aaggtcttcg aaagccacac cgggagcaat
     1381 attgccatcc ggatcaccaa ctgccgggtc ataaacataa ccgcatacat cacaaacata
     1441 cttcttcatt tttgcctcac ttgatcttgt tgataatttt atttcctatt tcgacaccta
     1501 aattaaaaca ttcagccaca accttttcat cgggctgata ctttgcattt actgcaccgg
     1561 caatttcaca acccatatcg gtaagatact gttttatctg agaaactcct tcaccgctcc
     1621 atccgtatga gccgaaagcc gcaccaaacg gagtctgaaa tttcaaccct tttaaatagg
     1681 tcagagcatc agcaactgat ggaaacaaat tgttgttaag cgttggcgtt ccgacaatta
     1741 ccgcagtcgc ttcaagcatt tctgtcgcta catcgcttct gcttgcagat tgaagcggaa
     1801 gcacttttac gcttactccg gtgcttaaca aaccatcttc aatatatctg gccatttttt
     1861 cagttgcatg ccacatcgtg tcataaacta tgaccgcttt tcttgaagtc gtccttgatg
     1921 accatctttg ataaagcccc agtacctttg agaagttatc catatttctc cacaccgggc
     1981 cgtgatcggg tgcgacaatc ttgaacgaaa gacctgaagt ctgaactttt ttgaacagcc
     2041 cttgaacaaa actgctgtag agcgtgataa tgttggcata atactccgct gccatttcgt
     2101 tgagtttcgt ccacgggatt tcatcatcaa atctttctga agaggcgtaa tgcatcccga
     2161 aagcatcctg actgaaaaga acctcctctt caacaagata tgtaaacatg ctgtcaggcc
     2221 agtgaagcat tctcgtttcc atgaatttca gcgttctgtt gccaagagaa agctcgcctc
     2281 cgtcatcaac agccgtgatc tcaacaccca tgtcaccaaa atgtgatttg agtgccttga
     2341 ccccattctt tgaagcgaac actttctgtg gcttaactgc cttaattatc tcaggaattg
     2401 tcccgctgtg atcgggctcc gaatggttgg agataatgta atctattctt tgcggatcta
     2461 caacagatgc aaccctttcg agcagttctt tttcaaaacc gtgctttacc ccgtcaataa
     2521 gcgtgatctt atctgccatt atcagaaagg cattgtaggt cgtgccgctt tttgtcgaat
     2581 agccgtgaaa gtatctcaca ccccagtcaa cagcaccaac ccaataaaca gaatcagtaa
     2641 cctttaccgc tttgaaatta ctagacattt actgctccgt tactgtgccg cagggggagt
     2701 aaatactctc gccgcaagtt ctttgtcgag catataaata ccgtgaggag cttctttcat
     2761 aagggcaagc tttttaatta cagcttcaac acttgcctct tcttcaacct gctcatcaac
     2821 ataccagtta atgaatgaac gg
//
LOCUS       DAOOYB010000340         2834 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_43499_length_2834_cov_8.474631, whole genome shotgun sequence.
ACCESSION   DAOOYB010000340 DAOOYB010000000
VERSION     DAOOYB010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43499_length_2834_cov_8.474631"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1187
                     /locus_tag="PL056_13110"
     CDS             <1..1187
                     /locus_tag="PL056_13110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM47999.1"
                     /translation="LSTSADIVFEGLWGTVDTPIGELKAGRMPFHWGLGILYNDGNRI
                     NHQSTGNYLDRIQLTIPVAGFKIIPAFDIASTGLLDKYQDYFIDSSQKDDGYNVSAMF
                     TMSEDDPALLENKLLNEQTVVEFGAMVMFSWKNKGSGEWKADTGTVEPLNDRYSDSDK
                     SYAFTKQDAKLWKIDGWFNLHHRNFSFKTELAFLTGSIGKMLLDDGTEKDVKAEMVGV
                     AIEMEYRAVPKKFHLSLLTGLASPDDAENVQADSWSTPGRSVNSSSVNTDAKVQNYRF
                     NKDYDFNSTLWNQFIGRFTAGYYASLYGTYYILDELKVDLGATYSMALKEENSFGGKG
                     LPSAIEPFVSIDYANKSGLRGGVRYQLGVPFSGLEVEDSEGEVVDAGLFHYLNVYLGI
                     VF"
     gene            1221..2135
                     /locus_tag="PL056_13115"
     CDS             1221..2135
                     /locus_tag="PL056_13115"
                     /inference="COORDINATES: protein motif:HMM:NF013665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HPM48000.1"
                     /translation="MFKSFDEIISEVKKNQKKQKVAVVGADKDIAIEAAADIVAENLG
                     EPVLFGPKEKIEKLWKGTVLENKVEIVDCFDPEMCCTRAVDYAVEGKVHLILKGTVET
                     AMLMKAIFRKENGLKDSPVISDVLVFEDPLGAENKRLIGLTDGGLIPLPDLETKIEMT
                     RSAVKVFHKLGFEKPKVAYISAVEKVSPKIQSTVDAAEIQKRKAAGELEDINAYLEGP
                     YAIDNVISEYAAKVKGIESVMAGSADILVMPNIESGNVLGKIANYWGNAANGHVITGA
                     KVPVLITSRADDAKTKLNSVALGILVSF"
     gene            2137..2409
                     /locus_tag="PL056_13120"
     CDS             2137..2409
                     /locus_tag="PL056_13120"
                     /inference="COORDINATES: protein motif:HMM:NF016262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein"
                     /protein_id="HPM48001.1"
                     /translation="MIHKKEEIRYPVTIDLKVIMTSESGDEKNISDLNKILSKFDVPL
                     KKEWIISKSNAGNYMCYTGRVYIGSKDVMYMLFEELKRHQSVKFAI"
     gene            complement(2420..>2834)
                     /locus_tag="PL056_13125"
     CDS             complement(2420..>2834)
                     /locus_tag="PL056_13125"
                     /inference="COORDINATES: protein motif:HMM:NF018009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HPM48002.1"
                     /translation="ELENGIVHNIDSFLRELGHEISYCGRQVPIKIDGLNHYIDILLY
                     HKGIPCNILVELKTKKLDARDVGQMNKYVSYFRINRQYEHEKETIGLIICSESGIEEA
                     QYALCGLEEKIFIATYQPKLPTMEQLKNAVRNVKR"
BASE COUNT          871 a          518 c          639 g          806 t
ORIGIN      
        1 agctgagcac ttcagcggac atagtttttg aagggctttg gggaacagtt gatactccga
       61 tcggtgagct taaggcgggt cgtatgcctt tccactgggg tctgggtata ctttacaatg
      121 acgggaacag aattaaccac cagtcgacag ggaactatct tgacaggatc cagctcacca
      181 ttccggtagc gggattcaaa ataattcccg ctttcgatat tgcatcaacg ggacttcttg
      241 acaaatatca ggattatttc attgattcat cacagaaaga cgacggatac aatgtcagtg
      301 caatgttcac aatgagcgaa gatgacccgg cacttcttga aaacaagctt ctgaatgagc
      361 agacagttgt cgaattcggt gcaatggtca tgttctcatg gaagaacaag ggttccggtg
      421 aatggaaagc agataccgga actgtggaac ctttaaacga cagatattcc gatagtgaca
      481 aatcttatgc attcacaaaa caggatgcaa aactctggaa aattgacggc tggtttaatc
      541 ttcaccaccg gaatttttct ttcaagacgg aactcgcatt ccttaccgga agtatcggaa
      601 agatgctcct tgatgacgga accgaaaagg atgttaaagc ggaaatggtc ggtgtagcga
      661 ttgagatgga atacagggcg gtgccgaaaa agttccatct gtcacttctc acaggtcttg
      721 cttctcctga cgatgctgaa aatgtgcagg cagattcatg gagcactccg ggaagatcgg
      781 tcaacagcag ttctgtaaat actgatgcca aagttcagaa ctacagattc aacaaggatt
      841 atgattttaa ttcaacactc tggaaccagt tcatcggaag attcactgcg ggatactatg
      901 caagtcttta cggtacatac tacattcttg atgaattgaa ggttgacctc ggtgctactt
      961 attcgatggc tctgaaggaa gagaattcat tcggtggaaa aggtcttcca agtgcgatag
     1021 agccattcgt ttcaattgat tatgcgaata aaagcggtct tagaggcgga gtcaggtatc
     1081 agctcggagt tcctttcagc ggtcttgaag tggaagattc tgaaggtgaa gttgttgacg
     1141 cagggctttt tcattatctt aatgtatatc tgggcatagt tttctaattt tttataaatt
     1201 ataattttca ggagcggatc atgttcaaat cttttgatga aattatttca gaagttaaga
     1261 aaaatcagaa aaaacagaaa gttgcagttg tgggagcaga caaggatatt gcaatagaag
     1321 cggcggcaga catagttgcg gaaaatcttg gagagccggt gcttttcggt ccgaaagaaa
     1381 aaattgaaaa actctggaaa gggacagttc ttgaaaacaa agttgagata gttgactgtt
     1441 ttgatcctga aatgtgctgt acccgtgcag ttgattatgc agttgaaggt aaagtccatc
     1501 tcattttgaa aggaactgtt gaaaccgcca tgctcatgaa agccatcttc agaaaagaga
     1561 acggtctgaa agattctccg gttatcagcg atgttctggt gttcgaagat ccgctcggag
     1621 ctgaaaataa gcggctcatc ggtctaactg acggcggact tattcctctt cctgatcttg
     1681 aaacaaagat cgagatgaca agaagcgcag tgaaagtctt ccacaagctc gggtttgaaa
     1741 aaccgaaagt tgcctatatt tcagcagttg aaaaagtgag tccgaaaatt cagtcaactg
     1801 ttgatgccgc tgagatacag aaaagaaagg cggctggaga gcttgaagat ataaatgctt
     1861 atcttgaagg tccttatgcg attgacaatg ttatttctga atatgccgca aaagttaaag
     1921 gaattgaatc agttatggca ggaagtgccg acattctcgt tatgcctaac atcgaatcgg
     1981 gcaatgttct cggcaagatc gcaaactact ggggaaatgc ggctaacggt catgtgatta
     2041 caggtgcaaa agttcctgtt ctcattactt caagagccga tgatgcaaaa acaaaactca
     2101 attcagttgc acttggaata ctcgtaagtt tttaatatga ttcataaaaa agaagaaatc
     2161 agatatcctg tcacgataga tcttaaagtt ataatgacaa gtgaatcagg tgatgagaaa
     2221 aacatatctg acttgaataa aattctaagt aaatttgatg ttccgctgaa aaaagagtgg
     2281 atcatttcaa aaagtaacgc cggaaactat atgtgttaca ccgggcgtgt ttatattggc
     2341 tcaaaagatg taatgtatat gctttttgaa gaacttaagc gacatcaatc ggtgaagttt
     2401 gctatttgag agtttagttt tatctcttca catttctcac agcgtttttc aactgttcca
     2461 tcgtcggaag tttcggttga tatgtcgcaa taaatatttt ttcctcaagt ccgcatagag
     2521 catattgagc ttcctcaatt ccagattctg agcagataat cagtccgatg gtctcttttt
     2581 catgctcata ctgccggtta attctaaaat aactgacata tttattcatc tgtccgacat
     2641 ctcttgcatc aagttttttt gttttcagtt caaccagaat attgcaggga attcctttgt
     2701 gataaagaag aatgtcaatg taatgattca aaccgtcaat ttttatagga acttgtctgc
     2761 cacagtaaga aatttcgtga ccgagttcac gcaaaaaaga atcaatgtta tgcacaattc
     2821 cgttttcaag ctct
//
LOCUS       DAOOYB010000341         2816 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_43892_length_2816_cov_11.873234, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000341 DAOOYB010000000
VERSION     DAOOYB010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43892_length_2816_cov_11.873234"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..710
                     /locus_tag="PL056_13130"
     CDS             33..710
                     /locus_tag="PL056_13130"
                     /inference="COORDINATES: protein motif:HMM:NF021540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2271 domain-containing protein"
                     /protein_id="HPM48003.1"
                     /translation="MRNQLILISLLTVIFAGCDNFDRTDFSFNPDSEEINDTNLTDDA
                     VDTAVTPDETAIPDETVAPDNTVTPDETVVPDENNTPIPVTMSLSVTTTSYNGSYAPR
                     NVFAVWIEKEDGSYIKTLGAWARNYKSKLQRWASKSNYGSNGMVDAVTGASRMNHNPV
                     PELTWKIDDIAFQPAENGIYNVYFELNETNSGSKSTTAKIQISESSEVIKANNTTNIK
                     DIQITFK"
     gene            complement(993..1883)
                     /locus_tag="PL056_13135"
     CDS             complement(993..1883)
                     /locus_tag="PL056_13135"
                     /inference="COORDINATES: protein motif:HMM:NF019371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HPM48004.1"
                     /translation="MKYTKPPKTFEEQADLLIERGLIVDRERLIKKLRHINYYRLSGY
                     LFPFKEKNEKFKENTTFEKVWRIYNFDRKLRLLIFDAIERIEVKSRTLAAYHIAHWNG
                     PFDYKNELLFPRLKVDHEKWIENLTNEYKRSQEVFKKHFENKYGDSHSLPPVWIAVEI
                     MSFGDIVRIFRSYNSNLTREFSAEIGIPDRVLESWLLSLNYIRNICAHHSRLWNRELS
                     IKPLLPNSRKYKDWDFLDYNAQSRIFVIIFICSFMLKHLSPSSRWNKRLLDLFNKYND
                     IPVSEMGFPENWKTCFPNES"
     gene            complement(2133..>2816)
                     /gene="uvrA"
                     /locus_tag="PL056_13140"
     CDS             complement(2133..>2816)
                     /gene="uvrA"
                     /locus_tag="PL056_13140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="The UvrABC repair system catalyzes the recognition
                     and processing of DNA lesions. UvrA is an ATPase and a
                     DNA-binding protein. A damage recognition complex composed
                     of 2 uvrA and 2 uvrB subunits scans DNA for abnormalities.
                     When the presence of a lesion has been verified by uvrB,
                     the uvrA molecules dissociate; Derived by automated
                     computational analysis using gene prediction method:
                     GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-ATPase UvrA"
                     /protein_id="HPM48005.1"
                     /translation="KEIFYSENRPFLSTTKLSTVATITGLSDLVKKKFGKGFKITKDD
                     LCKSCGGSGYTTVSLDYISDSESLCPDCEGSGYRGDILNQKYNGRNIYELLSMEVDTA
                     RKFFADDSKIMKILNIMNDCGLGYLHLDQQTNSLSTGEVQRLSLAVTLSKFLLGNDSE
                     SKFFLFDEPSTGLHFSDITHLLRLFRSLNEKGHTVIIAEHRNQIIQSADYIVELGPGS
                     GKDGGELLS"
BASE COUNT          893 a          547 c          505 g          871 t
ORIGIN      
        1 tttaaataaa cccgaattat acggagtaga aaatgagaaa tcagctcata ctaatttctc
       61 tgcttactgt aatttttgcc ggatgtgaca attttgatcg taccgacttt tcttttaatc
      121 ctgattcaga agagatcaat gacacaaatt tgactgacga tgccgttgat acagcagtta
      181 ctcccgatga aacagctata cctgatgaaa cagttgcgcc cgacaataca gttacacccg
      241 atgaaaccgt tgtgcctgat gaaaacaaca ctccgattcc tgtaacaatg tccctttctg
      301 tcacgacaac ttcttacaac ggttcttatg cacccagaaa tgtctttgca gtatggatcg
      361 aaaaagagga cggaagctac attaagactc tcggtgcctg ggcacgcaat tacaaaagca
      421 aactgcagag atgggcatca aaatcaaatt acggaagtaa cggaatggtt gatgcagtaa
      481 ccggcgcatc aagaatgaac cataacccag tgccagaatt gacctggaaa atcgatgaca
      541 tagcctttca acccgcagag aatggaattt ataatgtcta ttttgaactg aacgaaacga
      601 attccggctc aaaatccacc accgcaaaga tccagataag cgaatcatca gaagtcataa
      661 aggcaaataa cacaaccaac atcaaagata tccagatcac atttaagtag attttctttg
      721 atacgaattt tcagaggatc gtcaaattcg ctataataat tcgctatggt gataattata
      781 ttgatttcgt aggggcgaaa aatcttttgc cattacgatt cgcccattca aatcaattca
      841 attccgacac tcttgagtcc gattcaaaaa gattttaaca attgttattt tataggcaat
      901 ttatttataa tggctccctc tattcttgaa agagagggcg gttgaaaatc ccgcgcaaag
      961 ccgaagaggg atcgggttga gatgggcaag atttatgact catttggaaa acaggttttc
     1021 cagttttcag ggaaacccat ttcagaaacg ggaatgtcat tatatttatt aaataaatcc
     1081 aatagtctct tgttccacct gctacttgga gaaaggtgtt ttagcataaa tgaacatata
     1141 aaaattataa caaaaatcct gctttgagca ttataatcaa gaaagtccca atctttatat
     1201 tttcttgaat tcggcaaaag cggttttatg gaaagttctc tgttccacaa tcttgaatga
     1261 tgcgcacaaa tatttctaat atagttgagt gataaaagcc atgattctag gactctgtca
     1321 ggaattccta tttcagcaga aaattctctc gtgagatttg aattataact tctgaaaatt
     1381 cgcacaatat caccaaaaga cattatttca acagcaatcc ataccggagg aagagaatgt
     1441 gaatcaccat acttgttctc aaaatgtttt ttaaagacct cctgacttct tttatattcg
     1501 tttgtaaggt tctcaatcca tttttcatga tcaaccttaa gtcttgggaa aagcaattcg
     1561 tttttgtaat caaacggtcc attccagtga gcgatgtgat aagcagcaag agttcttgat
     1621 ttaacctcta tccgttcaat tgcatcaaaa ataagcaacc tcagttttct gtcaaaatta
     1681 taaattctcc atactttttc aaatgtcgtg ttttctttaa acttttcatt tttttccttg
     1741 aacgggaaaa gatacccgct tagacgataa tagttgatgt gcctaagttt ttttatcaac
     1801 ctttctctgt caacaatcaa gcctctttca attaataaat ctgcctgttc ttcaaatgtt
     1861 ttaggtggct ttgtgtattt cattattatg ctccggaaat aaaaaaaccc gccgaattac
     1921 gcatttccga agaaagaggc gcggcgggtg tgatgaggaa actttattcg caaaaatttt
     1981 tattgtcaag gatatcttta tacaaaactt caatatcatg ctaagattat tgcagattat
     2041 gttcattctt ttcaaaacca atcctcaaga aacataaatc aaattcgcat tattctaaac
     2101 tttaattggg agatatcaaa taaattagtg aattaggaaa gcagttcccc gccatctttg
     2161 ccgcttccgg gtccgagttc aacgatatag tctgcacttt gaattatctg gtttcgatgt
     2221 tctgcaatta tgactgtgtg ccctttttca ttaagtgatc tgaaaagcct gaggagatgt
     2281 gtgatgtcag aaaaatggag tcctgttgac ggttcgtcga ataaaaagaa cttactttct
     2341 gaatcattgc ccaagagaaa ctttgaaagg gtgacggcaa gcgaaagccg ctgaacttct
     2401 cctgttgaaa gggaattcgt ctgctgatca agatggagat aaccgagtcc gcagtcattc
     2461 ataatattca atattttcat gatcttgcta tcatcagcaa aaaatttgcg agctgtatcg
     2521 acttccattg aaaggagttc atatatgttc cttccgttat atttctgatt gagtatatcg
     2581 ccacggtatc cggaaccttc acaatcagga cagagagatt cagaatcact gatataatca
     2641 agtgaaactg ttgtgtaacc gctcccccca cagcttttgc agagatcgtc tttagttatt
     2701 ttaaaacctt tgccgaattt tttcttaaca agatcgctta agccggttat cgtcgcaaca
     2761 gttgaaagtt ttgttgtact cagaaatgga cggttttcgc tatagaaaat ttcttt
//
LOCUS       DAOOYB010000342         2805 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_44158_length_2805_cov_9.969455, whole genome shotgun sequence.
ACCESSION   DAOOYB010000342 DAOOYB010000000
VERSION     DAOOYB010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44158_length_2805_cov_9.969455"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(153..1205)
                     /locus_tag="PL056_13145"
     CDS             complement(153..1205)
                     /locus_tag="PL056_13145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48006.1"
                     /translation="MKKSMLLIMLLVFITGCDNETSEKNDNDTEPVHEIIGRYSDSFG
                     GPHIISNTVWDQGGMMGRFNIEFFDSENDVIIAQNDKNNEFSAEKWSRFDYLIDSSKI
                     YFCQIVYDLDTMQEAIDATGADRVDLDGGCNGFGWTELAEIAEESIDKDDALIVAWAN
                     GHESYIPGEDLIEDWKTPDKATGKAEGTSTDIVSLGNGGSIVLTFEKPITDGDGTDFA
                     VFENSLSDTFLELAFVEVSSDGTNFLRFDTYYLGVDPVDAFGNHDPELIWGFAGKFKQ
                     GLGTQFDLKELETKPEVVDNTVDISSITHIRIVDVIGDGSEIDTLGNPIYDPYPTTES
                     AGFDLDAVGVMNVVTD"
     gene            complement(1216..2403)
                     /locus_tag="PL056_13150"
     CDS             complement(1216..2403)
                     /locus_tag="PL056_13150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48007.1"
                     /translation="MKKLTVLMITLFSIGCSDGNFPDLPDGENVTADAPVLDLDVVEN
                     DDRSIESDLIVGTVGGYDVPFSGKVFLSENEKVQDLTPIGKDEKIGSSTGSDIAVSTW
                     DGGFFVIGRHDSSTVYFFDEKSTKDGFDFSAVDLGKGDYINLQEGVYNNFKDEFIFSA
                     LNSNYLIILKDNAVSKLQISENANASPAKMRIIKSKLFVAMQNLNESWQPGSGEMAVI
                     DMETYSVKRFGLTSKNPVGKIEYNQLVDSDHFYVACSGSWQKRDGAILRIDIETFEST
                     EILKESDNGGLLDGDFVDLTIADNGDFYIVFSNNSDRWINRLLKYDTKKGVVSQIDSG
                     VNAFAANPVDFSPVTKKIYYFADSGPDTFLRSVSTETGEIEETKLDEGPAFVKVWIRN
                     INN"
     gene            complement(2403..>2805)
                     /locus_tag="PL056_13155"
     CDS             complement(2403..>2805)
                     /locus_tag="PL056_13155"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPM48008.1"
                     /translation="KAAYTWLFGLKEYKVVEYFGEKYREETDLLRRPAHTFDAVLNYN
                     LMKKLNISLCINYVGERKDEVYNYPKTPYVVTADQFVILNIAISYKINKYLTIFGKIE
                     NMLDNDDYAYSVEYGTSGITPWLGLKFDTGE"
BASE COUNT          812 a          640 c          442 g          911 t
ORIGIN      
        1 tccagttaaa atcaaccgtg aaaacaccat cggaaatgct ttcaagaatt gattcataag
       61 ttatatgatc gcgtatttta accattttcc aacctaaaaa gatctttcgg ataggttata
      121 tcagatatcg ctaataaaat ccagaagggg atttaatctg taacaacatt catcacgccg
      181 actgcatcaa ggtcaaagcc ggcactttcc gttgtgggat aagggtcata gattggattt
      241 ccgagtgtgt ctatttcgct tccgtcacca attacatcaa cgatcctgat gtgtgtaata
      301 ctgctgatat ctacagtatt atcaaccact tctggttttg tttcaagttc tttgagatca
      361 aattgagtgc cgagtccctg tttaaatttt ccagcaaatc cccagatgag ttcagggtca
      421 tgatttccga aagcatcaac ggggtcaaca ccgagataat atgtgtcaaa tcttaagaaa
      481 ttagttccgt cactgcttac ttctacaaac gcaagctcaa gaaaagtgtc acttagactg
      541 ttctcaaaaa ctgcaaaatc ggtaccgtca ccatctgtaa ttggtttttc aaaagtcaga
      601 actatgcttc cgccgtttcc aagtgaaact atgtcagtcg aagtcccttc agctttacca
      661 gtcgctttgt caggtgtttt ccaatcttcg atcagatctt ctcctggaat atatgactca
      721 tgaccatttg cccatgcaac tatcagcgcg tcatctttat caattgattc ttcagcaatt
      781 tcagccagtt ctgtccatcc gaaaccgtta cagccgccat caagatcaac tctatcggcc
      841 cctgttgcat caattgcttc ctgcatcgtg tccaaatcat aaactatctg gcagaaatat
      901 atttttgacg aatcgataag gtaatcgaaa cggctccatt tttcagcact gaattcattg
      961 ttcttgtcat tttgagcaat gatcacatca ttttcgctgt caaaaaattc aatattgaac
     1021 ctccccatca ttccgccctg atcccagaca gtgtttgaaa ttatatgggg accgccgaag
     1081 ctgtcggaat atcttccgat aatttcgtgt acaggttctg tatcattgtc gtttttttca
     1141 gaagtttcgt tatcacatcc tgtaataaaa actaataaca taatcaacag catacttttt
     1201 ttcacaacaa cctccttaat tattaatatt tctaatccac actttgacaa aagcaggtcc
     1261 ttcatcaagc ttcgtttctt caatttctcc cgtttcagtg cttactgacc ttaaaaaagt
     1321 gtcaggtccc gaatctgcga aataatatat cttctttgtt actggactga aatctacagg
     1381 atttgccgca aatgcattta ctcctgaatc aatttgagaa acaactcctt tttttgtatc
     1441 gtatttaaga agtctgttta tccatctatc tgaattattt gagaaaacta tataaaaatc
     1501 accgttatcg gcaattgtca gatcaacaaa atcaccgtca aggagacctc cgttatcaga
     1561 ttctttaagt atctccgttg attcaaatgt ttcaatatct atccgcagga tcgcaccgtc
     1621 ccgtttctgc cagcttccgg aacaggcgac ataaaagtga tctgagtcaa ctaattgatt
     1681 atattcgatt tttccaacag gatttttaga ggtcaatcca aatcttttta cagaatatgt
     1741 ctccatatcg atcactgcca tctctcctga tcccggctgc cagctctcat tcagattctg
     1801 cattgccacg aacagtttgc ttttgatgat cctcattttt gcaggactcg cgtttgcgtt
     1861 ttcacttatc tgcaattttg acaccgcgtt atcttttaat attatcagat agttactgtt
     1921 taatgcactg aaaatgaact catctttaaa attgttgtaa accccttcct gaaggttgat
     1981 ataatcccct ttcccaagat caaccgcact gaaatcaaaa ccgtcttttg tcgatttttc
     2041 atcaaagaaa taaacggttg atgaatcgtg cctgccaata acaaaaaagc ctccgtccca
     2101 tgttgaaaca gcaatgtcag agcctgttga actaccaatc ttttcatctt taccgatcgg
     2161 ggtaagatcc tgaacttttt cattttccga caagaaaacc tttccgctga aaggaacatc
     2221 atatccaccg actgttccaa ctatcagatc actttcaatt gatctatcat cattttcaac
     2281 aacatcgaga tcaagtaccg gagcatctgc ggtcacattt tctccatcag gcagatctgg
     2341 aaaatttcca tcgctgcaac cgattgaaaa gagtgtgatc attaaaactg tcaatttttt
     2401 catcattcac ctgtatcaaa tttcagtcca agccacggag tgatccctga agtgccgtat
     2461 tccactgaat atgcgtagtc gtcattatca agcatattct ctatttttcc aaatattgtc
     2521 agatacttat tgattttata agaaatcgca atattcaaga tcacaaactg atcggcggtc
     2581 acgacataag gagttttcgg atagttgtaa acttcatctt ttctttcacc cacatagtta
     2641 atgcaaagcg aaatatttaa ttttttcatc aagttatagt tcaaaactgc atcaaatgtg
     2701 tgagcaggcc ttcttagcag atctgtttcc tctctgtatt tttcaccaaa atattcaaca
     2761 actttatatt ctttcagtcc gaataaccag gtatatgccg ctttc
//
LOCUS       DAOOYB010000343         2778 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_44780_length_2778_cov_7.586485, whole genome shotgun sequence.
ACCESSION   DAOOYB010000343 DAOOYB010000000
VERSION     DAOOYB010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44780_length_2778_cov_7.586485"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="PL056_13160"
     CDS             <1..642
                     /locus_tag="PL056_13160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48009.1"
                     /translation="QKDEEILAKYENVDPSAASGGSSSGGLSRLSSLPARSGKGGLNI
                     ISSKESENKDEKLNYFNIELKAALKFGIRSSGGTSVVAVLKEQKGEFPEGTVFYGSAT
                     FNNKRTFVNFTHAVVNDRKILLDGRAMMGKDPGIPSEVTEIAGDNANSSLKSGALGVA
                     GKVADNALSRVTGGATSGVLSEPTADLQKQQEQEKKQYDYYVPANTVFTIYVY"
     gene            663..1604
                     /locus_tag="PL056_13165"
     CDS             663..1604
                     /locus_tag="PL056_13165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48010.1"
                     /translation="MRKIVLTAVAVLLFFSVNAQEQNPEPEKFIEITEKVSVIKMLST
                     DTVIYRKVNITPTEVVVLEFPEGVLLDGDPSGSIAVGHDAILKTDVIPSPLVVKVTAM
                     INEVDLNSNLQIKLNCGITVIFNFVITLPEKASNRIIFTYPEFTEKNKKEKDAFLELK
                     LKLQKEHETAMKKVKEEANKLRVAGNAKGFSEFYMCNEYVNRSEKDLVFLTSTRICKW
                     GGKKDKGGDVYINFYIKNRYRNFFYVKEVKVYGLKGDSKIPIDNTQVFLEKFGIQFDE
                     VLNGAVGFELDDYYTQYRLELEEEAGKKRVIFVNVGF"
     gene            1616..2245
                     /locus_tag="PL056_13170"
     CDS             1616..2245
                     /locus_tag="PL056_13170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48011.1"
                     /translation="MKRILILISLLFALNTVSAEQGEFQLMFGAGGYFPLKQYTQNKN
                     VYIYASYNIPVAFYFGFTDNFDLGITGSFTRLTDTNVDTEYNGLSGREYFDYYHININ
                     GRLRYNWLPGYVFFAPHIFIGMGNNIENFSNRQFFVDGNKLYNDFNKGSYTDGTLNFQ
                     VGIDFTSRVWWFFLLNLELVYNHTLDNTNFFELNFYIGFDWFLKSYGGR"
     gene            2253..>2778
                     /locus_tag="PL056_13175"
     CDS             2253..>2778
                     /locus_tag="PL056_13175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48012.1"
                     /translation="MKKILFLIIAVFSVLTSCEDGGRPNTKDEFSIDIDGTSILKNAT
                     VSLFNDSDKEIMDGVTDENGLFVFEDVQSATGLLVKVCGGSFYSVATDSDVSFTGCLE
                     DSIPLSQGNVSVTVDILSTFISKYDSETSTEEWQTYLDITTLPSPALQSSLTDASKRY
                     LWYQGIAKIAENVSK"
BASE COUNT          844 a          565 c          609 g          760 t
ORIGIN      
        1 cagaaagacg aagagatcct ggcaaaatat gaaaatgtcg atccttcagc cgcaagcggg
       61 ggaagttctt caggcggtct ttcaaggtta agttcacttc ccgcccgttc aggtaaaggc
      121 ggacttaaca tcatttcttc aaaggaatct gaaaataaag atgaaaaact taattatttc
      181 aatattgaac tgaaagcggc tttaaaattc gggataagat caagcggcgg aacaagtgtt
      241 gttgcggttc tgaaagagca gaaaggcgaa ttccccgaag gaacggtatt ctacggcagc
      301 gccacattca acaacaaaag aactttcgta aatttcaccc atgcggttgt aaatgacaga
      361 aaaatacttc ttgacggcag ggctatgatg ggaaaagacc ccggaattcc ttcagaggtc
      421 acggaaattg ccggagataa tgccaattcc tcactgaaat ccggtgctct cggagttgcg
      481 ggcaaggttg ccgataatgc actttcaaga gttacgggcg gagcgacttc gggagttctt
      541 tcagaaccca ccgcagacct tcagaaacag caggaacagg aaaaaaagca atatgactac
      601 tatgtcccgg caaatacggt ttttacaatt tatgtatatt agcgaggttt taagctatga
      661 aaatgagaaa gatcgtcctc accgctgttg cggttctact gtttttttca gttaatgcac
      721 aggaacagaa tcctgagccg gagaaattca tcgagataac tgaaaaggtt tccgtgataa
      781 agatgctcag taccgatacg gtcatatacc gcaaggtcaa tatcacaccg actgaagttg
      841 tcgtacttga atttcctgaa ggagtcctcc ttgacggaga tccttccggt tcaatagcgg
      901 tcggtcatga tgccattctt aaaaccgatg tcatacccag tccccttgtc gttaaagtta
      961 ccgcaatgat aaacgaggtc gatcttaaca gcaaccttca gataaaactt aactgcggga
     1021 tcacggtcat tttcaatttt gtgataacgc tcccggaaaa ggcaagcaac aggataattt
     1081 tcacttatcc tgaatttacc gaaaagaaca aaaaggaaaa agatgctttc cttgaactga
     1141 agctgaaact tcagaaggaa catgaaaccg caatgaagaa ggtgaaagag gaagctaaca
     1201 aactgagagt tgcggggaac gcaaagggat tttcagaatt ttacatgtgc aatgaatatg
     1261 tcaacagatc cgaaaaagac cttgttttcc tgacaagtac aaggatttgc aaatggggcg
     1321 gtaaaaagga taaaggcggc gatgtctata taaatttcta tatcaagaac agataccgca
     1381 atttctttta tgtcaaagag gttaaagttt acggacttaa aggcgactcc aagatcccca
     1441 ttgacaacac acaggtattt cttgaaaaat tcggcataca gtttgatgaa gtccttaacg
     1501 gagcggtcgg atttgaactt gatgattact atactcagta ccgccttgaa cttgaggagg
     1561 aagccgggaa aaagagagtg atttttgtga atgtcggatt ttaaggggga tttttatgaa
     1621 aagaatttta atccttatct ccctgctttt cgccctgaac acggtttcag cggaacaggg
     1681 cgagtttcaa ttgatgttcg gagcgggcgg atattttccg ttaaaacagt acacgcagaa
     1741 taagaatgta tatatctatg caagctataa catccccgtt gcgttctatt tcggttttac
     1801 agataatttc gatctcggta tcacgggaag tttcacccgt cttacagaca cgaatgtcga
     1861 tactgaatac aacggtcttt cgggacggga atattttgat tactaccaca taaacattaa
     1921 cggacggtta agatacaact ggcttcccgg atatgttttc tttgcaccgc atatctttat
     1981 cggcatgggg aacaacatcg agaatttttc aaacagacag ttctttgttg acggtaacaa
     2041 gctttacaat gatttcaata agggctcata caccgacgga acacttaatt ttcaggtggg
     2101 aattgatttc acaagccgtg tgtggtggtt ctttctgcta aatcttgaac ttgtctataa
     2161 tcacacactt gacaacacca atttttttga acttaatttc tacataggtt ttgactggtt
     2221 tttaaaatca tacggcggga ggtaatttaa ccatgaaaaa gattcttttt cttatcattg
     2281 cggttttttc agttctcaca tcctgcgagg acggcggaag acccaacacg aaagatgaat
     2341 tcagcattga cattgacggc acttcaattc tcaagaatgc gacagtgtcc cttttcaatg
     2401 attctgataa ggagatcatg gacggtgtca ctgacgagaa cgggcttttt gtttttgaag
     2461 atgtccagtc tgcgaccgga cttctcgtta aagtatgcgg cggttcgttt tacagtgtag
     2521 ccactgattc agatgtcagt ttcaccggct gccttgagga ttcaataccg ctctctcagg
     2581 gtaatgtttc ggtcacagtt gacatactct caacattcat ttcaaaatat gattctgaaa
     2641 catcaactga agaatggcag acctaccttg atattactac acttccctcc cccgctcttc
     2701 agtcctctct tacagatgca agcaaacggt atctctggta tcagggaatc gccaagatcg
     2761 ctgagaatgt atcaaagg
//
LOCUS       DAOOYB010000344         2772 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_44914_length_2772_cov_9.264998, whole genome shotgun sequence.
ACCESSION   DAOOYB010000344 DAOOYB010000000
VERSION     DAOOYB010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44914_length_2772_cov_9.264998"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(242..2329)
                     /locus_tag="PL056_13180"
     CDS             complement(242..2329)
                     /locus_tag="PL056_13180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48013.1"
                     /translation="MRYLILAVIFGTIIFSCSNEKKGFYSEEGMNFHFDLNESGAKLK
                     KDLFLVTNGQKHLLVKHDDDSYELLINEYDALDYVQKDELTHYASGVKIKENSVNHIF
                     LTEGDPHFDPDHRLILSTYYIAREPVLRASSGLKDNNVKVCAVSKAVSMVFNHPELLT
                     SDPEKAEIIMKHINPVDQNMLCDKNAHHRPEIIDLAIAINTAEKNGGSVVNEPITVYS
                     ETEPDDESEKNVYWVKEPDDTESLIFFKDGNPFYVYKLTDDEEPAPIPFTGDISTKSP
                     AVQYRPVDDVLNAVTPALLVAMNEVKNNPLLEDVKYSIIKGDGPSETDSVIEIPDEEI
                     NDDEEMPDDDTVVLKTVLNSSGEKITLSKTGGIDGMSIYLNSDEIETDEKGFRYMTVK
                     VKNYYLRHVTLWTKWKKIDGTADFWWKKYLNDPNKDDSGLSPDFFSVVGLPSWYAFFN
                     DMASVKDSVFWGFVSNRAVMMGIPLSPDWSYFKIPIPPTSSKAELIFASMGTGKWQNE
                     NIPGAILTSVMDLGIPAYFLVSGAVDGVGGKPILKDALTNPKVITTIIALGISMGIYG
                     EAHKKGANNYAPYAKALGSILVNTGCDRLRYAIVKIVAKNQAEHAIPFVGWGLYIAGL
                     AANVSQLAQTVAEVSSTRAIHNNSLVATWSPKVRITPDPLNIRGGFPESAVKYEAYVH
                     FSDHDTKHLTGKT"
     gene            2450..2689
                     /locus_tag="PL056_13185"
     CDS             2450..2689
                     /locus_tag="PL056_13185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48014.1"
                     /translation="MKSIGLILTLLGAMAIIYYLVSIQSGPNIDKDIKESREVVIENE
                     KVDIRDLQKLKKSLDDQAKAEEERMRKEIEKATGE"
BASE COUNT          779 a          557 c          536 g          900 t
ORIGIN      
        1 ctatcctgtc tttatgaaaa tattcggttt tatcagtaat attgaccatg ttttctgtga
       61 tttgaacggt atagttgaca ccgtttttat ctgccggagc acttttcaga gtatttgtaa
      121 tttcatgatc aacatatccg gcaacaaaac cgctttcgct aagcagatta atttttattg
      181 ttgcgcttcc accttcagga atgtcaggga aagtgaaatc gaggtaagga actgcggtat
      241 ttcatgtttt cccggtaaga tgttttgtat catgatcact gaaatgaaca tatgcttcat
      301 attttacagc cgattcagga aaacctcccc tgatatttaa aggatcgggt gttatcctca
      361 ctttcggaga ccatgttgcg acaagactgt tattgtgaat cgcacgagtt gatgaaactt
      421 ctgcgacagt ttgagcaagc tggctgacat ttgcggcaag tccggctata taaagacccc
      481 agcccacaaa aggaatggcg tgttccgcct gattctttgc gactatcttg acaattgcgt
      541 atctcagtct gtcacaaccg gtatttacaa ggatcgagcc gagtgctttt gcataagggg
      601 cataattgtt ggcgcctttt ttatgtgctt caccatatat gcccattgaa ataccgaggg
      661 ctatgattgt ggtaataact ttcggatttg tcagtgcatc tttcaggatc ggctttcctc
      721 cgacaccgtc aactgctcct gaaacaagga aatatgcagg gattccgagg tccattacag
      781 aggtaagtat tgctcctgga atgttttcat tctgccattt tccagttccc atagaagcaa
      841 agatcagctc cgctttgcta cttgtcggag gaatcggaat tttgaaatat gaccagtccg
      901 gacttagagg tatgcccatc ataacggcac ggtttgaaac aaatccccag aagactgaat
      961 cttttactga agccatgtcg ttaaaaaaag cataccatga tggaagaccg acaactgaaa
     1021 aaaaatcggg agacaagccg gaatcatctt tattcggatc gtttaaatat tttttccacc
     1081 agaaatccgc tgttccgtcg atttttttcc attttgtcca cagcgtaaca tgccgcagat
     1141 aatagttctt cactttaact gtcatatatc tgaatccttt ttcatcagtc tctatctcat
     1201 cagaattaag atatattgac attccatcaa ttccgcctgt tttagaaaga gttatttttt
     1261 cacccgaact gtttaaaacg gtttttaaaa cgactgtatc gtcatcaggc atctcttcat
     1321 catcatttat ttcttcatca ggaatttcta tgacagaatc tgtttcagaa gggccgtctc
     1381 ctttaattat gctgtatttc acatcttcaa ggagcggatt attcttaact tcattcattg
     1441 ccacaagcag tgccggagtt acggcattca atacatcatc gacaggacga tactgaactg
     1501 ccggactttt tgttgaaata tctcccgtga aaggtatcgg agccggttcc tcatcatcgg
     1561 tcaatttgta tacataaaaa ggatttccgt ccttaaaaaa gatgagactt tctgtatcgt
     1621 cgggttcttt cacccagtaa acattttttt ctgattcatc atctggctct gtttcgctgt
     1681 aaaccgttat gggttcattt acgacgcttc caccgttctt ttcagccgtg ttaatagcta
     1741 tggcaaggtc gattatctca ggtctgtggt gagcattttt atcacaaagc atgttctgat
     1801 cgacaggatt tatgtgcttc attattattt cagctttttc cggatcgctg gtaagcagtt
     1861 ccggatggtt gaacaccatg ctgacagctt ttgacacggc acaaactttg acattattat
     1921 ccttcaatcc gcttgaagcc ctcagaacag gttcacgggc gatgtaataa gttgaaagta
     1981 tcagtctgtg atcaggatca aaatgaggat ccccttccgt taaaaatatg tgattaaccg
     2041 aattttcttt tatttttacc cctgatgcat aatgggtgag ttcatctttt tgcacataat
     2101 caagagcatc atattcattg atcagaagtt cgtacgaatc gtcatcgtgc tttaccagca
     2161 gatgtttctg tccgttagtt acaagaaaaa ggtctttttt caatttagct cccgactcgt
     2221 taagatcaaa gtgaaaattc atcccttctt cactgtaaaa accttttttt tcatttgaac
     2281 aactgaaaat tattgttccg aagatcactg ccagaataag atacctcatt gagttcctcc
     2341 ttttggaata agcacttttc acatttaatt atattacgga tgctttgttt acttacaagt
     2401 tttaaacagt ataatttaaa ggtaaaaaat gattttccgg gaggataaaa tgaaaagcat
     2461 cggtctgatt cttacactgc ttggagcaat ggcgataatt tattatcttg tttcgatcca
     2521 gtctggccct aacattgata aagacatcaa ggaatcaagg gaagtggtga tcgaaaacga
     2581 aaaagttgat atccgcgacc tccagaaact taaaaaatct cttgatgatc aggcaaaagc
     2641 agaagaagaa agaatgcgta aagagatcga aaaagcgacc ggggagtaga ttatgagaaa
     2701 gtttcttatg gttttattat tagtattttc tgtgaatttt ttgttttcag atgtgcccgc
     2761 aaatatttat ga
//
LOCUS       DAOOYB010000345         2765 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_45077_length_2765_cov_9.050923, whole genome shotgun sequence.
ACCESSION   DAOOYB010000345 DAOOYB010000000
VERSION     DAOOYB010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45077_length_2765_cov_9.050923"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..848)
                     /locus_tag="PL056_13190"
     CDS             complement(<1..848)
                     /locus_tag="PL056_13190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48015.1"
                     /translation="MNLKLIFTAILFSILISNLSLYGSDAKKNSDLKNAYKKEFAFLE
                     QQKKALQKMLDEYKKNSENQIEKGERETSALQDEFLKLSINSDSLQTALIEAERETSE
                     IVENKEVITNTIEQACSTLSKHGFKNEFSDDDKKSKGVEIMTDMFKRSVETLNDLSSI
                     KKENGEFYLVDGKAVNGDIIKIGAISAFGVSPEFSGSLAPAGNLAFKLWHESDPASAA
                     GIKDGKYLETVKMFVFESLEKEAEQKKSKGPIDVVRAGGAIAWVIMYLGLFTLLLIIL
                     RVAFLR"
     gene            complement(845..1666)
                     /locus_tag="PL056_13195"
     CDS             complement(845..1666)
                     /locus_tag="PL056_13195"
                     /inference="COORDINATES: protein motif:HMM:NF023358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3450 family protein"
                     /protein_id="HPM48016.1"
                     /translation="MKINIIRNAGGKPPAKFYLNKVKLLLMLLLSVSCFSSLKADELN
                     DLTSELIKIRGEVETLQNEIDIEKNALKNRMRAIELSKAELSNEIQRETLKIKQINLS
                     IASKKAQRGNPGKEEETIKNGVLDSVEIMKKHIENSLPFKVEERLSALNEISDQMNNG
                     AISSYRAVSRLWSFYEDEFRLTRENGLFSQPMEINGEKRIVTIARLGMIMAYYHYGES
                     DYGMFARESSGKWIVRPLTEKKHIELVASLFDSLKKQIRTGFFELPVLGVMEVKK"
     gene            complement(1684..2694)
                     /locus_tag="PL056_13200"
     CDS             complement(1684..2694)
                     /locus_tag="PL056_13200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48017.1"
                     /translation="MAEGTKEKPIVFTSAKEDGERARGDWGGLLVNGWAPVNCAGKTS
                     PCQRQAETGVEWYGGDKADDNSGTLKYVRIEFTGFKINSEKEFNGLTLQGVGNKTTIE
                     YIHTHMGNDDAIEFFGGTVNAKYILCTGAGDDNLDWTFGFSGKIQHVMIEQYEDEADK
                     GIEADTNDKQLDDTPVSMPTVSNVTIIGTANSSTGMIFRAGTGGKLHNVLVTGNPKCL
                     DIDEEQTFKYAEEGKLGFKNTILSCTKNFDMEDEKDEEENPIADAWPTSGQTLELWFE
                     GQTGNSVVADPKLDGWLPKSDSPALGNGVKPDDSFFDAVDYIGAFKDVDWTEGWTEFP
                     KN"
BASE COUNT          702 a          644 c          477 g          942 t
ORIGIN      
        1 cttcttaaaa atgccactct cagaattata agaagcaatg tgaaaagtcc gagatacatt
       61 atcacccatg ctattgctcc gcctgcacgg acaacatcga taggtccttt cgattttttc
      121 tgttcagctt ccttttcgag agattcaaaa acaaacattt taactgtttc aagatacttt
      181 ccatccttta tcccggcggc ggacgcagga tcgctttcat gccagagctt aaatgcaaga
      241 tttcccgccg gagcaagaga ccccgaaaat tcaggactta caccgaacgc acttattgcg
      301 ccgatcttaa tgatatcacc attaactgct tttccgtcaa caagatagaa ctcaccgttc
      361 tcctttttaa tagaagaaag gtcgttaagt gtctcaacag atcttttgaa catatctgtc
      421 attatttcta cacctttact ctttttatca tcatcgctga actcattttt aaatccgtgc
      481 ttggaaagag tggagcatgc ctgttctatc gtgtttgtaa taacttcttt attttcaact
      541 atttccgaag tttccctttc ggcttcaatg agagcagtct gaagcgaatc tgaattgatc
      601 gagagtttca agaattcatc ctgaagagca gaggtctctc tttccccttt ttctatctga
      661 ttctcactat tctttttata ttcatcgagc attttctgaa gtgctttttt ctgctgttcg
      721 aggaatgcaa actctttttt atatgcgttt ttaagatcgc tgttcttttt tgcatctgag
      781 ccgtaaagag ataaattaga gataagaatt gagaaaagaa ttgccgtaaa tatcaatttt
      841 aaattcattt tttcacctcc attacgccca gtaccggaag ctcgaagaac cctgttctga
      901 tctgtttttt caatgaatca aaaagtgacg cgacaagttc aatgtgtttt ttctctgtaa
      961 ggggcctgac gatccatttt ccgctgcttt ctctggcaaa cattccgtaa tcactctctc
     1021 cgtaatgata ataagccata atcattccaa gccgcgcaat tgtaacgatc cttttttcac
     1081 cgtttatttc catcggctgg gaaaaaagac cattctcacg ggtaagccgg aattcatctt
     1141 cgtaaaatga ccagagccgc gaaacagctc tgtaagaaga tatcgcacca ttattcatct
     1201 gatcagatat ctcgttaagg gcgctcagcc gttcctcaac tttgaaagga agcgagttct
     1261 caatgtgttt tttcattatt tcaactgaat caagcacacc gttttttatt gtctcttcct
     1321 ctttgccagg atttcctctt tgagctttct ttgaggcaat agaaaggtta atctgtttta
     1381 ttttgagtgt ttcccgctgg atctcgttgc ttagttccgc ttttgaaagt tcaattgctc
     1441 tcattctgtt tttaagagcg ttcttttcaa tgtcgatctc gttctgcagt gtttcaactt
     1501 cacctcttat tttgatgagt tcgctcgtaa gatcatttaa ttcatccgct tttaaagaag
     1561 aaaaacagct gacggaaaga agcagcatta acagtaactt aactttattt aaataaaact
     1621 tcgcaggggg cttcccccct gcgttacgga ttatgtttat tttcataact tattttcctg
     1681 agattagttt ttaggaaatt ccgtccagcc ctctgtccag tcgacatctt tgaatgcacc
     1741 gatataatca actgcatcaa agaaactgtc atcgggcttc acaccgttgc caagagcagg
     1801 agaatctgat ttcggcaacc atccatcaag tttaggatct gcaacaactg aatttcctgt
     1861 ctgaccttca aaccagagct caagtgtctg accgcttgtc ggccaagcat ctgctatggg
     1921 attctcttct tcatctttct catcttccat gtcaaaattc tttgtgcatg aaagaattgt
     1981 gtttttgaat ccgagttttc cttcttcagc atatttgaat gtctgttctt catcaatatc
     2041 aagacatttc ggatttccgg tgacaagaac attgtgaagc tttccgccgg ttcctgctct
     2101 gaagatcatt cctgttgaag aatttgcggt tccgataatg gtaacattgg atactgtcgg
     2161 catcgaaaca ggtgtatcat caagctgttt gtcattagtg tccgcctcaa tacctttgtc
     2221 tgcttcgtct tcatactgct cgatcattac atgctgaatt tttccgctga atccgaatgt
     2281 ccagtcaaga ttatcatctc ctgcacctgt gcagagaatg tattttgcat taactgttcc
     2341 accgaaaaac tcaattgcat catcatttcc catatgtgtg tgaatgtatt ctatggttgt
     2401 cttgtttccc acaccctgaa gtgtaagacc gttgaactct ttttcagaat tgatcttaaa
     2461 ccctgtgaac tcaattctca catatttcaa tgttccgctg ttatcatcag ctttatctcc
     2521 gccgtaccac tcaacaccgg tttccgcctg cctctggcat ggagaagttt ttcctgcaca
     2581 attaacagga gcccatccgt ttacaagaag tccgccccag tcgcctcttg ctctttcgcc
     2641 atcttctttt gcagatgtga aaacaatcgg tttttccttt gttccttcag ccatgatctt
     2701 gcttccagtg caagcatgat caacaaaaaa ccgagaatct tttttacctt catttttcta
     2761 ctcct
//
LOCUS       DAOOYB010000346         2763 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_45122_length_2763_cov_8.100812, whole genome shotgun sequence.
ACCESSION   DAOOYB010000346 DAOOYB010000000
VERSION     DAOOYB010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45122_length_2763_cov_8.100812"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..1276)
                     /locus_tag="PL056_13205"
     CDS             complement(101..1276)
                     /locus_tag="PL056_13205"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HPM48018.1"
                     /translation="MKKKLFQGADNFNFSLLKSRKTALIVNHTSVDRNGVSTVEKMVA
                     SGVNVTAIFSLEHGYFPVAQDMENVGKENKISNIPVFSLYGDSVHSLKPDTSFFDMFD
                     VAVYDIQDIGSRYYTYLASLAMFMDELHKHPKELIILDRINPIGGTVEGSVLDDSIYK
                     SFVGYFPLIHRHGLTSAEVAKYYHSLRKYDFPLKIEVVTGWARDSFMDEYDYPWMPTS
                     PNMPTVDAAVLYPGGCLLEGTELSEGRGTTFPFSVAGFPGIDPFILKKELDKLKIEGL
                     TFIPLEFRPMFQKQAMKRCGGVYISLTDRSVVKPLRAYISIIQTFRRMIGESGFFRTK
                     PYEFIEDIPAIDLLLGDRELIDMFYNFANMKDIDEHLKKCEKKADEIFKQFRIYQEK"
     gene            complement(1281..2036)
                     /locus_tag="PL056_13210"
     CDS             complement(1281..2036)
                     /locus_tag="PL056_13210"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylcholine/phosphatidylserine synthase"
                     /protein_id="HPM48019.1"
                     /translation="MIKNNPKVLIPNFFTVLNMLIGLTSIFYSIKGDYIDAGWLILLS
                     GVMDKLDGTAARFFKVSSMFGVEFDSYSDFISFGFAPAILVFSYFNAQGTGGFSDASF
                     YAVAASAFYVVFCAVRLAKYNVSQSDDKEYFYGLTTTMSGGFIALYMIFAIENGFEWL
                     LTVNLVAGMMMAHSIMLLIPFKYPKLKKPSSRSAQIIMLFSFALFLGLILVRILPWLI
                     YITGVGYLVIGTLKTKKAFVHVVPEDSENEPSE"
     gene            complement(2073..>2763)
                     /locus_tag="PL056_13215"
     CDS             complement(2073..>2763)
                     /locus_tag="PL056_13215"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HPM48020.1"
                     /translation="ILLLVSTGFEPLLAKKKMRIAVLELSPVKVGADVAGGVTDLVVT
                     ELVNCGEFEVLERMQVAKILNELGFQQTGVTDTSKAIEAGKLLNTESVMIGTLQQFNS
                     SLVINVKIVEVSTGKILFADKAVADNNDKLIDASSNLVTSLVSRMTGSAPAKKQVQPQ
                     QQQQQQQSQNTSPDQLPVRVFYAKPAQGNNQGTTQSGSQKGSAQENKKEDKDSKTKRN
                     ANESFNELENE"
BASE COUNT          805 a          587 c          544 g          827 t
ORIGIN      
        1 ttcccacagt ttcttctttc tttccgcttt caattcctgt aagtgcctga aaaaccgagc
       61 ttttgcccga aaatggaagg ccgataagcg ctgctctcat ttatttctcc tgatatatcc
      121 tgaattgttt aaaaatctca tctgcttttt tttcacattt ttttaaatgt tcatcaatat
      181 ctttcatatt tgcaaaatta tagaacatat ctattaattc cctgtctccg agcagaaggt
      241 caattgctgg aatatcttca atgaattcat agggttttgt tctgaaaaaa ccggattcac
      301 cgatcattct tctgaatgtc tgaataatcg aaatataagc tctaagcggc ttaacaactg
      361 atctgtcagt aagtgatatg taaacgccgc cgcacctttt cattgcctgt ttctggaaca
      421 tcggcctgaa ttcaagcgga atgaaagtta aaccctcaat tttcaattta tccagctctt
      481 ttttgagtat gaaaggatca attcccggaa agcccgcaac agaaaaagga aatgttgtcc
      541 cgcgaccttc agaaagttca gttccttcaa gaagacatcc gcccggataa agaactgcgg
      601 catcaactgt cggcatattt ggagatgtcg gcatccacgg atagtcatat tcatccatga
      661 acgaatctct tgcccagccg gtaaccactt caattttcaa gggaaaatcg tattttctca
      721 aactgtggta atatttagca acttcagcgg aggtgagccc gtgccgatga attagaggga
      781 aatatcccac aaaactttta tatatgctgt catcaagcac agatccttca acggttccac
      841 cgatagggtt tattctgtca agaattatca gttctttcgg atgtttgtga agttcatcca
      901 tgaacatggc cagtgacgca agataggtgt aatatctgct tccgatatcc tgaatgtcat
      961 ataccgcaac atcaaacatg tcaaaaaaag aagtgtccgg tttaagagaa tgaactgaat
     1021 ctccgtaaag tgaaaaaacc ggtatgttgc tgatcttatt ctcttttcct acattttcca
     1081 tatcctgtgc aactggaaaa taaccgtgtt caagtgaaaa gatagcagtg acattcaccc
     1141 ctgaagcgac cattttctca acagttgaaa caccgttcct gtcaacggaa gtatgattaa
     1201 cgatcagagc cgtctttctg cttttaagca aactgaagtt gaaattgtcg gcaccctgaa
     1261 acagtttctt tttcatggtg tcattcggag ggttcatttt cgctatcttc aggaacaaca
     1321 tgaacaaagg ctttctttgt tttcaatgtt ccgataacaa gatagcctac acctgttata
     1381 taaataagcc atgggagtat tctgacaagt ataagcccga gaaaaagggc aaaagaaaaa
     1441 agcataataa tttgggcaga tctggaagag ggttttttta acttcggata tttgaacggg
     1501 atcagaagca ttattgaatg agccatcatc attccggcaa ccagattgac agtcaaaagc
     1561 cattcaaatc cgttttcaat ggcaaagatc atgtaaaggg caataaaccc gcctgacatg
     1621 gtagttgtaa gtccataaaa atattcctta tcatctgact ggcttacatt atattttgca
     1681 agccgcacag cacagaaaac tacataaaaa gcacttgccg caactgcata gaagctggca
     1741 tcagaaaatc cgccggtacc ttgtgcgttg aaatatgaaa aaacaaggat cgccggagca
     1801 aaaccgaaag aaatgaagtc ggaataggaa tcaaattcca caccgaacat gcttgatact
     1861 ttgaaaaatc tggcggcagt tccgtcaagt ttgtccataa cgccggacag aagaataagc
     1921 catccggcat caatataatc accttttata gaatagaata ttgatgtgag tccgatgagc
     1981 atgttcagaa ccgtgaaaaa attgggaata agaactttcg ggttattttt gatcatttcg
     2041 gccccggaat ttaaaaagat taaaataaag gtctattcat tctcaagttc attaaagctt
     2101 tcgttggcgt tccgtttggt ttttgagtct ttgtcttcct ttttgttttc ctgggctgac
     2161 cctttctgac tgccactctg tgttgttccc tgattgtttc cctgtgccgg tttagcatag
     2221 aaaactctga cgggaagctg atcgggagaa gtattctggc tctgctgctg ctgctgctgc
     2281 tgctgtggct gtacctgttt ttttgcaggg gctgaaccgg tcattctgct gacaagagaa
     2341 gttacaagat tggatgatgc atcaataagt ttgtcgttat tgtccgccac agctttgtct
     2401 gcaaaaagga tcttgcctgt gcttacttca acgatcttta catttattac aagactggag
     2461 ttaaactgtt gtaaagttcc tatcattacc gactctgtat taagaagttt acctgcttct
     2521 attgcttttg aagtgtcagt cacgccagtc tgctgaaatc cgagttcgtt aaggatcttg
     2581 gcgacctgca ttctttcaag aacttcaaat tctccgcagt tcacaagttc ggtgacaaca
     2641 agatcggtaa ctcctccggc aacatctgca ccaactttga ccggtgaaag ctcaagcact
     2701 gcgattctca tttttttctt tgccagaagc ggctcaaaac ctgttgacac cagaagaagg
     2761 atc
//
LOCUS       DAOOYB010000347         2752 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_45372_length_2752_cov_12.786429, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000347 DAOOYB010000000
VERSION     DAOOYB010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45372_length_2752_cov_12.786429"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            140..922
                     /locus_tag="PL056_13220"
     CDS             140..922
                     /locus_tag="PL056_13220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48021.1"
                     /translation="MIRKVLGIFMVFAVMMFFAACDNEAQNSNEDNTDDRKNGEETDN
                     EKENPDDDDEDLTKGVLSGCLGKEGEDYYPVPQKVSLFDKSKNKIVFYYQDKFYCAGN
                     DFYYKAVPDQTDKTLLNVYLKAVNIMDGSVPGCDCPMRIEMEFSSEDHDLTNIEKIKI
                     DYDFYWAEQQEDKVFSFEKINCFYNGKIYEEDNNLTDWCNLCVCTKYGDVACDEMSCG
                     TCESGETIMLTCENDKEIEWCKCPDPEKGWKCLEDPISLCEE"
     gene            complement(987..2405)
                     /locus_tag="PL056_13225"
     CDS             complement(987..2405)
                     /locus_tag="PL056_13225"
                     /inference="COORDINATES: protein motif:HMM:NF013776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM48022.1"
                     /translation="MFIGRRNELNMLEESYKSDKSELVVIYGRRRIGKSRLVKEFGKD
                     KSHFFSFEALENSSMAEQMNHFVKQMSGQINDPFINNINFDSWHDIFSYLTTKLIKNS
                     RRQKKLVVFFDEIQWMAGKRSTLISLIKYFWDKEWKDQNIMLIMCGSIASFMIDKVIN
                     SKALYGRMTMEMHLKGLQLNEIPAFFSSDTSNEEIIKYISVFGTVPKYFEMIDKNRSF
                     EQNINKLCFSGNAVMITEMEKIFYSQFQEAANYIQIVNLLKKGMLNLKEISEKTGMPS
                     GGGLKRYIENLEKADIVKSFVPWNKKSDSKIKKYSIIDEFLNFYFRYIEPNKVDITGT
                     NRGNLFKSYTSDSFAVWLGLAFEKMCVKHSIFLADIMGFKNEVASSGSHFSIDNDRFQ
                     VDLIYKRFDKVVTICEIKHHTKELSAEIIKEMERKKQLLKIPRGYSVETALISTYGPD
                     KSLKSSGYFNHFVTAQDIFSKR"
BASE COUNT          834 a          471 c          507 g          940 t
ORIGIN      
        1 cgcggaggta cttactaaat taggccgctt aaaatgatct gcaaccgttt ttggctttct
       61 ctgacaaaac ataaataaaa tcctggaatt attgtactaa agttataatg ttttgttaat
      121 taactttccg gaggaagtca tgattagaaa agtgcttgga atttttatgg tttttgctgt
      181 tatgatgttc tttgccgcat gtgataatga agctcagaat tcaaatgaag ataacacaga
      241 tgatagaaaa aacggcgagg aaaccgacaa cgaaaaagaa aatcctgatg atgacgatga
      301 agatcttaca aaaggtgtcc tcagtggttg tttgggaaaa gaaggggaag attactatcc
      361 agttcctcag aaagtgagct tgtttgataa gagcaaaaac aaaatagtgt tttattatca
      421 ggataaattc tactgtgccg gaaacgattt ttattataag gcagttcctg atcaaacaga
      481 caaaactctg ctcaatgtgt atttgaaagc ggtaaacatc atggacggga gtgttccggg
      541 gtgtgactgt ccaatgagaa tcgaaatgga gttcagttca gaagatcatg atcttacaaa
      601 tattgaaaag ataaagattg actatgattt ctactgggct gaacaacagg aagacaaagt
      661 tttttccttt gaaaagatca actgttttta caatggaaaa atttatgaag aagataataa
      721 tctgacagat tggtgcaatc tttgtgtctg tactaagtat ggtgatgttg cctgtgatga
      781 aatgtcttgc ggcacatgtg aaagcggtga aacgataatg cttacatgtg aaaatgataa
      841 agaaattgaa tggtgcaaat gtcccgatcc tgaaaaagga tggaaatgcc tggaagaccc
      901 gatttctttg tgtgaagagt gagacgccgg ctaaccacct gtatcaattc actttcttca
      961 gtttccatgc gaactgaata attcctttat cttttgctga aaatgtcctg tgctgtaaca
     1021 aaatggttga aataaccgct tgatttgagc gatttgtcag gtccgtaagt gctgatgagt
     1081 gcagtttcga cgctatatcc gcgtggaatt tttaacaatt gtttctttct ttccatttct
     1141 tttattattt ctgccgataa ttcctttgtg tggtgtttga tttcacagat tgtaacaacc
     1201 ttgtcaaatc ttttgtaaat aagatctacc tgaaatctgt cgttatcaat tgagaaatgt
     1261 gatccggatg aagctacttc gttcttaaaa cccataatat ctgcaagaaa aatagagtgt
     1321 ttgacgcaca ttttttcaaa agcaagcccg agccatacag caaacgaatc tgatgtatat
     1381 gatttaaata aatttcctct gtttgttccg gttatatcca ctttattcgg ctctatatat
     1441 ctgaaataaa aatttaaaaa ctcgtcaatg atagaatatt ttttgatttt gctgtctgac
     1501 tttttattcc agggaacgaa tgattttaca atgtccgcct tttcgaggtt ttcaatatac
     1561 cttttaagtc cgccaccgga aggcatgcct gttttttcag aaatttcctt taggttcagc
     1621 attccttttt taagcagatt aacaatttgg atatagtttg cagcttcctg aaactgactg
     1681 tagaatattt tctccatctc tgttatcatc acggcattac cggaaaaaca cagcttgttg
     1741 atgttctgtt caaatgaacg gtttttgtcg atcatttcaa aatattttgg aacggtgcca
     1801 aaaacagaaa tatatttaat aatttcttca ttgctggtgt cagatgagaa aaatgccgga
     1861 atttcattca gttgaagtcc tttcaggtgc atttccattg tcattctgcc gtacaatgct
     1921 tttgaattaa ttactttatc aatcataaaa gatgcaattg agccgcacat gatcagcatt
     1981 atgttctgat ctttccattc cttgtcccag aaatatttta tcaatgaaat gagagtgctt
     2041 cgtttccccg ccatccattg aatttcatca aagaaaacaa caagcttttt ttgtctgcgg
     2101 ctgtttttta ttaattttgt tgtaaggtag gaaaaaatat catgccatga atcaaaatta
     2161 atgttgttga taaaaggatc attaatttgc cccgacatct gttttacaaa atgattcatc
     2221 tgttcagcca tgctgctgtt ttcgagggct tcaaatgaaa agaaatggct tttatctttt
     2281 ccgaattctt tgacaagccg gcttttaccg attcgccgtc tcccatatat tacaacaagc
     2341 tcactcttgt cggatttata gctttcttca agcatattaa gctcatttct tcttccgata
     2401 aacatgtgta actcccttga aattaacatt ttttcaaatt tcattgtatt taacttgtgc
     2461 ggataagcaa gttttaaaat caattgtccg cacaacatga aagctttcca acttgtgcgg
     2521 ataaggcatt ttatgaaaga agctaagcgg gttttcccta attgtctatt atccacagta
     2581 actgatttct aatcttatcc aatctgtttt ctaatggctt cctaatcata tattaacact
     2641 tctgctttac tttctttccg gctcattgag cgttatgtaa aacgataact ttttctaagg
     2701 agtagaaaaa tgaaggtaaa aaagattctc ggttttttgt tgatcatgct tg
//
LOCUS       DAOOYB010000348         2752 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_45377_length_2752_cov_5.305525, whole genome shotgun sequence.
ACCESSION   DAOOYB010000348 DAOOYB010000000
VERSION     DAOOYB010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45377_length_2752_cov_5.305525"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..128
                     /locus_tag="PL056_13230"
     CDS             <1..128
                     /locus_tag="PL056_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011218799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HPM48023.1"
                     /translation="ILLIADTISRTVIAPSEIPVGVITALLGGPFFLLVLIKKKG"
     gene            214..1497
                     /locus_tag="PL056_13235"
     CDS             214..1497
                     /locus_tag="PL056_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002735128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPM48024.1"
                     /translation="MAQIEGFRIKNFKALKDVTIGKLWNTQYAKPLRPLTVVIGKNGV
                     GKSSLFDAFGFLADCLKFGVEEACDMRGRGGFEKIKSKGQSGPIEFELYFTADKGARP
                     ITYELAIDLDRHKRPFVTKERLRQRRKGQKHGWPFSFLILENGKGVAWKGETDGIQEE
                     KTNYTIEDIFKIIVNPEKEEGKNTEKVELDDNRKLGISTIGSLKQHQRISFFRKFIEG
                     WYLSYFTPDAARSLPLAGPQKKLNIHGDNLGNVVQYMEREHKDKFQKILNQISAKIPG
                     ITSIDTEKTPDGRLLLRFNSQGFRDPFYSQQMSDGTLKVFAYLLLLEDPDPTPFLCIE
                     EPENGLYHKLLESLATEFRNKATGKKNSSQIFITTHQPYFVDALDPEEVWILDKGEDG
                     FSTIRRASDDELVKNMVAEGLPLGSLWYSDYLDAR"
     gene            1502..2152
                     /locus_tag="PL056_13240"
     CDS             1502..2152
                     /locus_tag="PL056_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005370368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4276 family protein"
                     /protein_id="HPM48025.1"
                     /translation="MHFEIYVEDASGKKFLDLVIPMIFGSEHTFKVHSYKGIGHIPKN
                     MKKPEDASKRILLENLPKILKGQGRTFQGYRDEYKAAVVILCDLDKKCLKQFRQELLN
                     ILEQCDPKPETAFCIAVEEMEAWLLGDVNAIKKAYPKAKTNILVSYENDSICDTWERL
                     ADAVYTGGATKLLGKGWQTVGREKSVWAEKITPHLNINDNKSQSFQYFVKKLLSLI"
     gene            2269..>2752
                     /locus_tag="PL056_13245"
     CDS             2269..>2752
                     /locus_tag="PL056_13245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48026.1"
                     /translation="MKTLTATVMVKAGRRNGRGGLDALTLSGDDTLFVGGGVSGKDGF
                     YTLNAFNLNGEQKFEKLGESNGGRIFSLITDLDGNLYAGGVVNSQAFLIKFSESGEKI
                     WTNYLNEKESNSGTYALALDKNNNIYAADNSASSGVKGNITKFSPEGKELWHHNISTE
                     D"
BASE COUNT          954 a          405 c          615 g          778 t
ORIGIN      
        1 tgatccttct gatcgctgac actatatcaa gaactgtcat cgcaccctca gaaattcctg
       61 tcggggtcat aaccgcactt cttggcggac cgttctttct gttagttctg ataaagaaga
      121 aaggttgaaa aataaagagt tttgttttta ttttttttga ataaaataac ttcgaaagtt
      181 tttgtttaat aattgaagga gacggagaat aaaatggcac agattgaagg attcagaatt
      241 aaaaacttca aagcattgaa agatgtaact attggtaaat tatggaatac gcaatatgca
      301 aaacctttaa gacctcttac cgttgtaatt ggaaagaatg gagtcggaaa aagttctctt
      361 tttgatgcat tcgggttttt ggcagattgt cttaaattcg gcgtggaaga ggcttgtgat
      421 atgcgtggaa gaggcggttt tgagaaaatt aaatcaaaag gtcaatccgg acctatagaa
      481 tttgaacttt attttacggc tgataaaggc gcaagaccta ttacatatga acttgccatt
      541 gatttggata gacataagcg tccattcgta actaaagaga gacttcgtca aagaagaaaa
      601 ggacaaaagc acggatggcc attctctttt ttaattctcg aaaacggcaa aggggtggca
      661 tggaaaggtg aaacagacgg aattcaggaa gaaaaaacta actatacgat agaagacatt
      721 tttaaaataa ttgttaatcc tgaaaaagaa gaaggaaaaa atacagaaaa agttgagctc
      781 gatgacaaca ggaaacttgg tatcagtaca attggatcac tcaagcaaca tcagaggata
      841 tcttttttca ggaaattcat tgaaggttgg tatttgagtt attttacacc tgatgccgcc
      901 agaagtcttc ctttggccgg accacagaaa aaacttaata ttcatggtga caatcttggc
      961 aatgtagtac aatatatgga aagagaacac aaagacaaat tccagaaaat actcaaccaa
     1021 atatctgcaa agattccggg aattacaagc attgatacgg aaaaaacacc ggatggcaga
     1081 ttgttgctga ggtttaacag tcaaggtttt agagaccctt tttattcgca acagatgtct
     1141 gatgggactt taaaagtgtt tgcatattta ttgcttctgg aagaccctga ccctactccg
     1201 tttttatgta tagaagagcc ggaaaatggt ctttatcaca aacttcttga aagtcttgcg
     1261 acggagttca gaaataaagc aaccgggaaa aagaactctt cacaaatctt tattacaacg
     1321 caccagccat attttgtaga tgcactcgat ccggaagagg tgtggatttt agacaaagga
     1381 gaagatggat tttcgacaat aagaagagca agtgacgacg aacttgtaaa aaatatggtc
     1441 gcagagggac ttcctctcgg atctttgtgg tacagcgatt atcttgatgc gaggtagtca
     1501 gatgcatttt gaaatatatg tggaagatgc atcagggaaa aaatttttag atttggtaat
     1561 tcccatgatt tttggttccg aacatacatt taaagttcat agttataaag gaattggtca
     1621 tattcccaaa aatatgaaaa aacctgaaga tgcatctaaa aggattttgc tcgaaaactt
     1681 gccgaaaata ttaaaaggtc agggaaggac atttcaaggc tatagggatg aatataaagc
     1741 cgcagttgta atattatgtg atcttgataa aaaatgcctt aaacagttca ggcaggaatt
     1801 gcttaatatt cttgaacaat gtgatccaaa accggaaaca gcgttttgta ttgcagtaga
     1861 agaaatggaa gcttggcttc ttggcgatgt aaatgcaata aaaaaagctt atccgaaagc
     1921 aaaaacgaat atattggtga gttacgaaaa tgattctatt tgtgacactt gggagaggtt
     1981 ggctgatgct gtttataccg gcggtgcaac aaaattgctg gggaaaggtt ggcagacagt
     2041 gggtagggaa aaatctgtat gggctgagaa aataacaccg catcttaata taaacgacaa
     2101 caaatctcag agttttcagt attttgtaaa aaaactacta tcattaatat aatacagact
     2161 cacaaattta tgatagcatt atcagtctga acacggaggg agggatattc atgagaacat
     2221 taattttggc attaacagtt atgatgatgt tattatcatg tgttgaagat gaagacatta
     2281 acggcaacgg tgatggtaaa ggctgggaga aggaatggga ggggggggtt agatgctttg
     2341 acgttaagcg gcgacgatac ccttttcgtt ggaggcgggg tttctggcaa agatggtttt
     2401 tatactctta atgcatttaa tctaaatgga gaacagaaat ttgagaaact tggggaaagt
     2461 aatgggggaa gaattttttc acttattaca gatttggatg ggaatttata tgcgggcgga
     2521 gtagttaatt cacaagcttt tttgattaag ttctctgaaa gcggtgaaaa aatatggacg
     2581 aattatttaa acgaaaaaga atcaaattcg ggaacatacg ctttggctct ggacaaaaac
     2641 aataatattt atgctgctga taattctgcg agttccggag ttaaaggtaa cattacaaaa
     2701 ttctcccctg aaggaaaaga gttgtggcat cataatattt caacagaaga tg
//
LOCUS       DAOOYB010000349         2727 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_45987_length_2727_cov_9.767216, whole genome shotgun sequence.
ACCESSION   DAOOYB010000349 DAOOYB010000000
VERSION     DAOOYB010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45987_length_2727_cov_9.767216"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            207..2249
                     /locus_tag="PL056_13250"
     CDS             207..2249
                     /locus_tag="PL056_13250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48027.1"
                     /translation="MKKTMLSVCIISAFLLSCDFGLLDNQGVEEIVSEKNELLSNGPV
                     DDSPDDKTLVYDKDRTVVEYFENCAVTLNKSKTLTRLDIMDMKKSDPDLVEEIFPNRS
                     VAMKAITGRLDKDVSFFPSIETVNGKLKKFNDALYAGVELALEEGVNDFPSKPDFLNK
                     VAIKLNDLEKDVSQTGMAHINSAKVYIAVAMKLSNIEDIPFSSEILKEADSVINEMDK
                     DFFLQPMGFYTWSDELKMIFKRDRYLQNYHDDEDPYTEETVGRAIALSLAITAVPELE
                     TEYDQILGVYSDLTNPFADIPVTALKSYIIGIGALDQVSSITKSFLSDNPADYIGSSC
                     NNHFALIPASYSKETDYFQSITCRDGYAGGINFMDELIAAIKDGKIDLEPDDDSGWYD
                     YQSYALETLLLPERGEENNNLLLTKAYKEKLIESFKSIMIQNRETHAKTLEMGASKYT
                     YAEPKPFDIYPKLPVEPFATFYLRNARAYRFLNTALTAITGDDFMDEVKVHYEKGESD
                     LSLSDGLNYMTMLMYGLAFISADSVGAEMTLLEDELSKSEIEACRTDAKNWLAEWTED
                     SDIRTDPRVAVPVQYDYNNNNVTYWAVIGVRIIRATTEFHKDFLPEIVKIGGDGEESW
                     DCKFREFLPHDYYMMIEKSAEFTVDMDKPVLDRETFRALCNKGTTEKEILEELANY"
     gene            2246..>2727
                     /locus_tag="PL056_13255"
     CDS             2246..>2727
                     /locus_tag="PL056_13255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48028.1"
                     /translation="MKKLIICLSFIVMTSCGYGMMQTAKTVEKGKSSYSFGTVYQGNK
                     ERPFYVYSISGQFNFRHGVHDRVDFGIKQFFIGGLLADAKVNVLDPKSPFALSFSSGI
                     GFAVDPINVDIILQVPVFVHLSYTVKDTFTPYATFGYETNWIFRKEKPENIELEEGFE
                     "
BASE COUNT          839 a          527 c          585 g          776 t
ORIGIN      
        1 ttgtcttcct gctgctcagt tttcaatgac ctcaaaaaag cgctcgaaca atatatgaat
       61 ctaaaaaaca ttgtcaacac tttttttatc ttttttgcat tttttattca agtgcccgtt
      121 tttaatggtt tatattttct tttgataaaa aaactcctct ctgtatacta aaagtgctgt
      181 gagaaacttt taaacaggtg ttcataatga aaaaaacaat gttaagtgtc tgcattattt
      241 cagctttcct gctttcatgc gacttcggac tgctcgacaa tcagggcgtt gaagagatcg
      301 tgagcgaaaa aaacgaactg ctttcaaacg ggccggtcga tgattcgcct gatgacaaaa
      361 ctctcgttta cgacaaggat agaactgttg ttgagtactt tgagaactgt gctgtaaccc
      421 tcaacaagtc aaagactctc acccggctcg atatcatgga tatgaaaaag agcgatcccg
      481 atcttgtgga ggagatattt ccaaacagat ccgtcgcaat gaaagcgata accggccggc
      541 ttgataaaga tgtttcattt tttccatcta ttgaaactgt caacggaaaa ctgaaaaaat
      601 tcaatgacgc tctttatgca ggggttgaac tggctcttga ggagggtgtg aatgattttc
      661 cgtcaaagcc cgatttttta aacaaagttg cgatcaagct caatgatctt gaaaaagatg
      721 tcagccagac tggaatggca catataaatt cagcaaaggt ttatattgca gttgcgatga
      781 agttatcaaa tattgaggat attccgttca gttctgaaat tttaaaagag gccgattctg
      841 taataaatga gatggataaa gattttttcc ttcagccgat gggtttttac acatggtctg
      901 atgaacttaa gatgatcttt aagagagacc gctatcttca gaactatcac gatgatgaag
      961 atccttatac tgaagagact gtcgggagag cgatcgcact ttcccttgct attactgctg
     1021 tccctgaact tgaaactgaa tacgatcaga tactcggtgt ttattccgat cttaccaacc
     1081 cttttgcaga tattccggtc accgccctta aatcttatat tataggtata ggggctcttg
     1141 atcaggtctc ttccattaca aaatctttcc tttcagataa ccctgctgat tatattgggt
     1201 cgtcctgcaa caaccatttt gcacttattc cggcaagtta cagcaaagaa accgactatt
     1261 ttcagtcgat cacctgccgg gatggttatg caggagggat aaacttcatg gatgaactta
     1321 tcgcggcgat taaggacgga aaaatcgatc ttgagccgga tgatgattcg ggctggtacg
     1381 attatcagtc gtatgcactt gaaacactgc ttctgcctga acggggagaa gagaacaaca
     1441 accttctcct tacaaaggca tacaaggaaa agctgattga aagttttaag agcatcatga
     1501 tccagaatcg cgaaactcat gcaaaaacgc ttgaaatggg cgcatctaaa tatacttatg
     1561 cggaaccgaa accttttgac atctatccga aactgcctgt tgaaccgttt gcgacttttt
     1621 atttaagaaa tgcaagagct tacagatttc ttaatactgc tctgactgcc ataactgggg
     1681 acgatttcat ggatgaagtc aaagtgcatt atgaaaaggg tgaatcagat ctctctttga
     1741 gtgacgggtt gaattatatg accatgctta tgtacgggct ggcatttata agtgccgatt
     1801 cagtcggtgc tgaaatgacg cttcttgaag atgaacttag caaaagtgaa attgaagcgt
     1861 gcagaactga tgcaaaaaac tggcttgcag aatggacaga agacagcgac ataagaacag
     1921 atccgagagt tgccgttcct gttcagtatg attataataa caacaatgtc acttactggg
     1981 cggttatcgg ggtcaggata atccgtgcaa caactgaatt tcacaaagat tttcttcctg
     2041 aaatcgttaa gataggtggc gatggcgaag agagctggga ctgtaagttc agggaattcc
     2101 tgccgcatga ttactatatg atgatagaaa aaagtgctga atttacagtg gatatggata
     2161 aacccgtcct tgaccgcgaa actttcagag cgctgtgcaa caaagggaca actgaaaaag
     2221 agattttaga ggagcttgca aactattgaa aaaactcatt atttgtcttt cattcattgt
     2281 tatgacaagc tgcggatacg gaatgatgca gacagcaaaa acagttgaaa agggaaaaag
     2341 cagctactcg ttcggaacag tttatcaggg aaataaggaa agaccgtttt atgtttattc
     2401 aataagcgga cagttcaact tcaggcacgg agttcacgac cgcgttgact ttggaataaa
     2461 gcagtttttc atcggcggac tgctcgcaga tgccaaagtg aatgtccttg accccaaatc
     2521 gccgtttgca ctttcattca gttcaggaat cggcttcgcc gttgacccaa taaatgttga
     2581 cattatattg caagtgcccg tttttgttca tttaagctac acagttaaag acacattcac
     2641 accgtatgca actttcggat acgaaacaaa ctggatattc cgcaaagaaa aacctgaaaa
     2701 cattgaactt gaagagggct ttgaatt
//
LOCUS       DAOOYB010000350         2702 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_46552_length_2702_cov_8.675859, whole genome shotgun sequence.
ACCESSION   DAOOYB010000350 DAOOYB010000000
VERSION     DAOOYB010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46552_length_2702_cov_8.675859"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..779)
                     /locus_tag="PL056_13260"
     CDS             complement(<1..779)
                     /locus_tag="PL056_13260"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HPM48029.1"
                     /translation="MKINRTIFRQYDVRGHVNVDLTEDFAYSLARGCASYYKKELPSI
                     KKVSIGHDARESSPLFAKALIKGFNDSGIDTVFIGMVPTGLLYFSMFNLDVQASVMIT
                     GSHNPPEYNGFKINLIDLPVYGDGIQKIADIMERDDFIDAKVKGKNSGYNIIPDFEKY
                     ILKNIKIKNPQKLKIVVDSGNGVGGFVAVPILRKMGVNVISIFEEPDGTFPNHHPDPT
                     VEKYIQDMIKTVKKEKADFGIGYDGDADRIGLVDRNGKIIWG"
     gene            complement(795..2129)
                     /locus_tag="PL056_13265"
     CDS             complement(795..2129)
                     /locus_tag="PL056_13265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48030.1"
                     /translation="MKLNLFIAVISAVFFFMGCSSGESKTNDDSKTDDFVKTDDEAVI
                     DNEVQDDAVDPVCGNATVETGEVCDGGEIDCAKLDSAKWGSGKAACKADCSGYDEANC
                     VEIVLCGNETVDTGEECDGTGSLCSELGMGYLTGVAECTADCKFDTSTCLPQCGNGEL
                     EEGEDCEYKEGEETQDSISCHKLSWLEYKSGDAVCGTDCKWDKTACVALDVVPQYGMI
                     SQLGVDTVKGVTDAKKLCVPKTDGTDGCDFDATFALNHIGDTTFNITGSYKNNSKIIL
                     TAPSGFDIITKDSKQACLAVNKGFDPLPEKFFTLFASIAFDRLTFQNIKMYGPQVVLM
                     FNSETIPNTTAGVKDYYNISGIGDDDILLAIGEQMGDGTTDIDVNSLCIAAIGFGKKM
                     EVSNAFNVMADGGGGTITYSGFDIPLYHPTETPLGDLTEMVKEYGLENICDK"
     gene            2350..>2702
                     /locus_tag="PL056_13270"
     CDS             2350..>2702
                     /locus_tag="PL056_13270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48031.1"
                     /translation="MKVKFLTVIMMIFAVFLFSCENSSTSTSSKKKNPFDDESDRVIK
                     TGPLEVNEAKIYISYESDKYFLNIPVKLESGSSSINAEISVNVNKLDGTAMDNLSRSH
                     SETVTSLTSEIVMELG"
BASE COUNT          756 a          612 c          496 g          838 t
ORIGIN      
        1 ccccagatga tctttccgtt gcggtcgaca agccctattc tgtctgcatc accgtcataa
       61 ccgataccga aatctgcttt ttctttttta acggtcttga tcatatcctg aatatatttt
      121 tcaactgtcg ggtcgggatg atggttcggg aaagttccat ccggctcttc aaagatactg
      181 attacattga cacccatttt ccttaagatc gggactgcga caaatcctcc tacaccgtta
      241 cctgaatcga caactatctt cagtttctgc ggatttttaa tctttatgtt tttgagaata
      301 tacttttcaa aatcgggaat gatgttgtat ccgctgttct tccctttgac tttcgcatcg
      361 atgaaatcgt cacgctccat gatgtcagcg atcttctgga tgccgtctcc gtaaaccgga
      421 aggtcaatga gattgatctt aaaaccgttg tattccggcg gattgtggct tcctgttatc
      481 atgacagatg cctgaacatc aaggttgaac atcgaaaaat aaagaagacc ggtggggacc
      541 attccgatga aaactgtatc tattccgctg tcgttgaatc ctttgatgag tgcttttgca
      601 aaaaggggtg aactttccct tgcatcgtga cctatgctaa ctttcttaat tgaaggaagc
      661 tcttttttgt aatatgaagc acatcccctt gcaaggctgt atgcaaaatc ctctgtcaga
      721 tctacattga catgtccgcg aacatcgtac tgtctgaaaa ttgtcctgtt gattttcatt
      781 ttagtctccc tgttctattt atcgcaaatg ttctcaagtc cgtattcctt aaccatttca
      841 gtaagatctc caagcggagt ttcagtcggg tggtaaaggg gaatgtcaaa gccggaatat
      901 gtgatagttc cgccgccgcc gtcagccatt acattgaaag cgttgctcac ttccatcttc
      961 tttccaaaac cgattgcggc aatgcagaga gaattcacat caatgtcagt tgtaccgtca
     1021 cccatctgtt cacctatagc aagaagaata tcgtcatcac cgattccgct gatgttataa
     1081 taatccttta ctccggcagt cgtgttggga attgtttcgg aattgaacat gagaaccacc
     1141 tggggtccgt acatttttat gttctggaat gtcaatctgt cgaatgcaat tgaagcaaaa
     1201 agggtaaaaa atttctctgg aagcggatcg aatcctttgt taactgcaag gcaggcctgt
     1261 ttactatctt ttgtaataat atcaaaccct gaaggtgcgg taagaataat ttttgagtta
     1321 ttcttataac ttccggttat gttaaatgtt gtgtctccaa tatgattcaa tgcgaaagtt
     1381 gcatcaaaat cacaaccgtc ggttccgtct gttttaggaa cacaaagttt ttttgcatca
     1441 gtaacacctt taacagtgtc aactccaagc tgagagatca ttccgtactg tggaactaca
     1501 tcaagagcta cacatgcggt tttgtcccat ttgcagtcag ttccgcagac agcgtcaccg
     1561 cttttgtatt caagccagct taatttatga caactgatgg aatcctgagt ctcttcacct
     1621 tctttatatt cgcaatcttc accttcctca agttcaccgt ttccgcactg cggaagacat
     1681 gttgaagtgt caaatttgca gtccgcagta cattcagcaa cacctgtcag ataacccatt
     1741 ccaagctctg agcagagcga gccggttccg tcacactctt cgcctgtatc gacagtttca
     1801 tttccgcaca gaactatttc aacacagttc gcttcatcat atcctgaaca gtcggctttg
     1861 catgccgctt tccctgaacc ccatttagcg gaatcgagtt ttgcacagtc aatttcaccg
     1921 ccgtcacaaa cttcaccagt ttcaacggtt gcgtttccgc agactggatc aaccgcatca
     1981 tcctggactt cattatctat caccgcctcg tcgtctgtct tcacgaaatc atcagttttg
     2041 gaatcatcat ttgttttgct ttcaccactt gaacagccca taaaaaagaa aaccgctgaa
     2101 ataacagcga taaacagatt gagtttcatc ttccactccc tatcaaaaaa agtaataaac
     2161 ctgaatacgc agacatttta cataatttaa cataaataac aattttaaga cacaataaaa
     2221 gaagtccgtt tttttattcc ccgttttctt tgaaaaaaaa atgctccaga gtaaaatatt
     2281 taaataaatt tccgctgttt atggaactaa atagccactt gtttttttta acaatatggg
     2341 gagaaaacca tgaaagttaa gtttttgaca gtaattatga tgatctttgc tgtttttctg
     2401 ttttcctgtg agaattcgtc aacatcaaca tcttcaaaaa agaaaaaccc ttttgacgac
     2461 gaaagcgatc gtgtaatcaa gacaggtccg cttgaagtaa atgaagctaa gatctatatt
     2521 tcctacgaat ctgataaata ttttctcaac attcctgtca aactggaatc aggatcgtcc
     2581 tcgataaatg cagaaatttc agtcaatgtg aataaacttg acggaacagc catggacaac
     2641 ctttcaagat cgcattctga aacggtaact tcgctgacca gtgaaatagt catggaactt
     2701 gg
//
LOCUS       DAOOYB010000351         2687 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_46948_length_2687_cov_6.160334, whole genome shotgun sequence.
ACCESSION   DAOOYB010000351 DAOOYB010000000
VERSION     DAOOYB010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46948_length_2687_cov_6.160334"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1134
                     /locus_tag="PL056_13275"
     CDS             <1..1134
                     /locus_tag="PL056_13275"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPM48032.1"
                     /translation="GDDALDQMQTCSEGIWSNRGNCLEPGKYAILEKDAVKTDMTIDL
                     EATPAGTDIALIVDTSGSMSDDISVLKNNISTVTSNIRSYIPDSRFGLVSLGTLGYSP
                     YLPIQNITDDLTTFNSKVATIPSGSGSYEYHTVTIEQTASGSGTTQTVKTYDGGSDYS
                     VNVSSASCGSGFRGGICFRENSLPLFVLMSDEAFATSGWIWSVGAETTVQNAVDSMND
                     INAKIAVIDSSGISKYLETNTNTLAAGTYSYDQAGDPFYYNIPTTGTEINLKIEDAVK
                     RMYDETEMTAGLVFESPVSNTIQSTQFVLSHATVSADPAENIHSKDDLYFYGTLRETM
                     LRYSISLRNTVSTPSQMTFLTLKIKLKWYSITLDTIDFTIVIP"
     gene            1155..1931
                     /locus_tag="PL056_13280"
     CDS             1155..1931
                     /locus_tag="PL056_13280"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HPM48033.1"
                     /translation="MFRKYALILPLMLLTLFLNAEDLKNKKNARISITTVPAGLNVYI
                     DGIFAGKSPLNNFEVLKTAKLLEVTDNSGKKVIYSADLDLKNGKIIERKEKGYDLKKK
                     TEKKEQKKEQKKEPEKQQKKQPEKKPEVKIPGPHKWTGTGFIIAGILTAGLGSLFDYL
                     AVKEFDKYKKMNDKDNLMKAMMTGNFNEAEYIEKSNSHRTKGQNYSAARTVLFVAGGA
                     SVITGIILIFIPEKKKKIEMLSFGVSPYEDGFFISAGGHF"
     gene            1934..>2687
                     /locus_tag="PL056_13285"
     CDS             1934..>2687
                     /locus_tag="PL056_13285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48034.1"
                     /translation="MRCKIPALILFMAIFFAGCAKDPGEMRIKETIADIEKEKAEKCG
                     DGVTDSWEECDYSGNLSCSEHSDELLGPLRCTECRIDISGCAEKKKCDSDICSGNGLC
                     TEGNSHYVYCKCFALFTGPTCDRCIDNYHFDLDGKCINNTECTLVGCMENSTCQIISG
                     KAECVCNDHYAGPDCDACEAGYYMDNGICKGALCSNTGLVCTEFEKCDDSSGKPRCIC
                     KGENQNPQDCSECDEGYGWYKNEACLKEKWVIC"
BASE COUNT          895 a          547 c          588 g          657 t
ORIGIN      
        1 ggggacgatg ctcttgacca gatgcagact tgttctgaag gtatctggtc aaacagagga
       61 aactgccttg agccgggaaa atatgcgatc cttgaaaaag atgctgtgaa aaccgacatg
      121 acaattgatc ttgaagcaac acctgccgga acagacattg ctctcatcgt cgatacaagc
      181 ggttcaatgt cagatgatat ttctgtctta aagaacaata taagtactgt tacatcaaat
      241 atcagatctt atattccaga ctcccggttc ggacttgttt cacttggaac actcggatac
      301 agcccctatc ttcctattca gaacataaca gacgatctaa ccacattcaa ttcaaaagtt
      361 gccacgattc ccagtggcag cggatcatac gaataccaca ccgttacaat tgaacagact
      421 gcaagcggtt caggcacaac tcaaactgtc aaaacatatg acggtggatc agattacagc
      481 gtaaatgttt cctctgcaag ttgcggatca ggttttcgcg gaggaatatg tttcagagaa
      541 aattcacttc ctctgttcgt cctcatgtca gatgaagctt ttgcgacaag cggatggata
      601 tggagcgtcg gagccgaaac cactgttcag aatgccgttg attccatgaa tgatattaat
      661 gcaaagatcg ctgtgatcga ttcaagcggc atttcaaaat atcttgaaac caacacaaat
      721 acactggcgg caggaactta ttcctatgat caggcgggag atccttttta ttacaacatc
      781 ccgacaacgg gaacagaaat aaaccttaaa atcgaagatg ccgtaaagag aatgtacgat
      841 gagaccgaaa tgacagcagg actcgttttt gaatcacctg tcagcaacac aatccaatca
      901 acccagtttg ttctcagcca cgcaactgtt tcggcagacc ctgcggagaa catccattca
      961 aaggacgatc tgtactttta cggaacttta agagagacca tgctcagata ctcgatctca
     1021 cttcgaaata ctgtttcaac accatcccag atgacatttc tcacattgaa aataaagttg
     1081 aaatggtaca gcattacgct tgacacaatt gatttcacga tcgtaattcc ttaataaaac
     1141 gggggagagg ttttatgttc agaaaatatg cactgatcct gccgttgatg cttctcacat
     1201 tgtttctgaa tgctgaagat ctcaaaaata aaaaaaatgc gcggatctcg ataactaccg
     1261 ttcctgcagg acttaatgta tatattgacg gaatttttgc cggaaaaagt ccactgaaca
     1321 actttgaggt tctgaaaacg gcgaaactcc ttgaagttac cgataattct ggaaaaaaag
     1381 ttatttattc cgcagacctt gacctgaaaa acggcaaaat catcgaaaga aaagagaaag
     1441 ggtacgatct caagaagaaa acagaaaaaa aagaacaaaa gaaagaacaa aagaaagagc
     1501 cagaaaaaca acaaaagaaa caaccggaaa aaaaacctga agtaaagatc ccgggtcctc
     1561 acaaatggac agggacgggt tttatcattg caggaattct cactgctggg cttggctctc
     1621 tttttgacta tcttgcggtg aaagaatttg ataaatacaa gaaaatgaat gacaaagata
     1681 atctgatgaa agcaatgatg acgggaaatt ttaatgaggc ggaatatatc gagaaaagca
     1741 actcccaccg gacaaaaggg cagaactact ctgctgcaag aaccgttctt tttgtggcag
     1801 gcggagcatc cgtaatcact ggaatcattc tcatctttat tcccgaaaag aaaaaaaaga
     1861 tagagatgct ttcatttgga gtatcgcctt atgaagatgg cttttttata agcgcaggag
     1921 gacatttctg atcatgagat gtaaaatacc ggctctcata ctttttatgg caatcttttt
     1981 tgccggatgt gcaaaagacc ctggcgaaat gagaattaaa gaaactattg cagacatcga
     2041 aaaagaaaaa gcggaaaaat gcggagacgg cgtaactgac agttgggaag aatgcgacta
     2101 ttcaggcaat ctttcatgca gcgaacattc tgacgaactg ctggggccgc tcagatgcac
     2161 tgaatgcaga attgacattt ccggctgtgc tgaaaaaaag aagtgtgatt cagacatatg
     2221 tagtggtaac ggcttatgca ctgaaggaaa cagccactac gtttattgta aatgttttgc
     2281 actttttacc ggtcctacct gcgacagatg catagacaac tatcatttcg accttgacgg
     2341 aaaatgcatc aacaacaccg aatgcactct cgtaggatgc atggaaaaca gcacctgtca
     2401 gataatttca ggaaaagccg agtgtgtgtg caacgatcat tatgctgggc ctgactgtga
     2461 tgcgtgtgag gcgggttact acatggataa cggcatctgc aaaggagcat tatgttccaa
     2521 caccggtctt gtctgtactg aattcgaaaa atgtgacgac agttccggca agcccagatg
     2581 catctgcaaa ggggaaaatc agaatccgca ggactgctca gaatgtgatg aaggatacgg
     2641 ctggtacaaa aatgaagcgt gtcttaagga aaaatgggtg atatgtg
//
LOCUS       DAOOYB010000352         2681 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_47115_length_2681_cov_13.131379, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000352 DAOOYB010000000
VERSION     DAOOYB010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47115_length_2681_cov_13.131379"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..468)
                     /gene="ndk"
                     /locus_tag="PL056_13290"
     CDS             complement(10..468)
                     /gene="ndk"
                     /locus_tag="PL056_13290"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008150491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HPM48035.1"
                     /translation="MAREKTLVLIKPGAIQRELAGEIITRFERKGIKIVGVKMMMLDD
                     YMIEKHYSHLRNEPFFNELKKGMQRTPIIALALEGNDIIKRVRVLIGATNPIEAAPGT
                     IRGDYSISLSNNVIHASANAEEALIELQRFFKEDELFNYQRMNVMDYTED"
     gene            612..1562
                     /locus_tag="PL056_13295"
     CDS             612..1562
                     /locus_tag="PL056_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020674596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HPM48036.1"
                     /translation="MKRNFFNQNFYGIREIGTYIPEKRINNTLKQEKFGFKESFLEDK
                     IGVFKTSLKDDNEDCSDMCVKAFNDLVSKIPIEKNEIEVIIVVTQNPDDRMPHSGALV
                     HKKLGMGVNCASFDISLGCSGFIYGLSVITSFMKANNMKKGILFTSDPYSKIIDPDDR
                     NTTLLFGDGATATLITNDEPVIVPGKFSFGTLGEKWEEIRLINDKLFMNGAAVVKFVK
                     RYVPDDIRKNIEDNGYMLENIDRFVLHQASKFMVDIVAEDLGVNPSKVPFDIRNYGNT
                     ISSSIPLVLEKELHNKENRTILISGFGVGLSYGSCVLKRV"
     gene            1688..2266
                     /locus_tag="PL056_13300"
     CDS             1688..2266
                     /locus_tag="PL056_13300"
                     /inference="COORDINATES: protein motif:HMM:NF036995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 N-terminal domain-containing protein"
                     /protein_id="HPM48037.1"
                     /translation="MDIEKLVNLFQDTHNELQSRAARSVDTYLVVRNWLFGWYIVEFE
                     NGGAERAELYGKELIKKLSEKLTANLGKGFSKRSLEQFRQFYLTYKEITQALPAQYEK
                     RANAISPTVSDKCEIVHEFEMLHNVQTVSGISENLISETVPRISKSDSLVELTLPKDS
                     NIYASSYQLYLPSKEELKKQIEEAEREFSKNK"
     gene            complement(2343..>2681)
                     /gene="cutA"
                     /locus_tag="PL056_13305"
     CDS             complement(2343..>2681)
                     /gene="cutA"
                     /locus_tag="PL056_13305"
                     /inference="COORDINATES: protein motif:HMM:NF015077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HPM48038.1"
                     /translation="METKMVMIVSAFPSKEVARKIALEMIEKRIASCIQITAPVLSIY
                     EWEEKICQEDEVLAFVKTSCSLQDSAVQFIKERHPYEVPEIITIPVIGGFDKYIQWVE
                     DNLISVKRES"
BASE COUNT          825 a          509 c          551 g          796 t
ORIGIN      
        1 ggattttgct cagtcttcag tgtaatccat gacattcatt ctctgataat tgaaaagttc
       61 atcttccttg aaaaaccgct gaagttcgat gagtgcttct tcagcatttg ccgatgcgtg
      121 gatgacattg ttgcttaatg agatggagta atcacctctg atggttccgg gagccgcttc
      181 tatcggattt gttgcaccga tgagaacccg cactcttttt ataatgtcgt tcccttcaag
      241 ggcgagagcg attatgggag ttctctgcat tccttttttg agttcattga agaaaggttc
      301 gttgcgaaga tgggaataat gtttctcgat catatagtca tccagcatca tcattttgac
      361 tcctacgatt tttattccct tcctttcaaa gcgggtgata atttcaccgg cgagctcccg
      421 ctgaatcgct cccggcttga taagcaccaa ggttttttct cttgccatta tgattgcctc
      481 cataaaaaag ttgcgttata actttatcac ttatttgaca taactacaaa ttttaatatt
      541 tttcagtaac cggtcgagag atcggtcagt caaaaaaggg gatgggttta aattttttat
      601 ctggagcggg gatgaaaaga aattttttta atcagaattt ttacggaatc agggagatag
      661 gcacttacat tcctgaaaaa aggatcaaca acacattgaa acaggagaag ttcggtttca
      721 aagaatcatt tcttgaagat aaaatagggg tttttaaaac ttccttgaaa gatgataacg
      781 aagactgttc agatatgtgt gtcaaggcgt tcaatgacct tgtgtcaaaa attccgattg
      841 agaaaaatga aattgaagtc attattgtcg tgacacagaa tccggatgac agaatgcctc
      901 attcaggagc acttgttcac aaaaaactcg gtatgggggt aaattgtgca tcatttgaca
      961 tttctcttgg atgctccggc ttcatttacg gactttcggt cattacaagt ttcatgaaag
     1021 cgaacaacat gaagaaaggg atccttttca caagtgatcc ttattcaaaa ataattgatc
     1081 ccgatgacag gaacacgacg cttcttttcg gtgacggagc gacagcaact ctgataacaa
     1141 atgatgaacc ggttattgtt ccggggaaat tttcgtttgg aacactcggt gaaaaatggg
     1201 aagagatccg gctcataaac gacaagcttt tcatgaatgg tgctgctgtt gttaaatttg
     1261 ttaaaagata tgttcctgac gatattagaa aaaatattga agataacgga tatatgcttg
     1321 aaaatattga cagatttgtt cttcatcagg caagcaagtt catggttgac attgtcgcgg
     1381 aagatctggg tgtcaaccct tcaaaagtac cgtttgacat aagaaattac ggcaacacca
     1441 tctcaagttc aattccgtta gtccttgaaa aagaattgca caataaagag aacagaacca
     1501 tcctcatcag cggtttcgga gtcggcttaa gttacggaag ctgtgtcctg aaaagggttt
     1561 gaactttttc acagccattc cacatttaca cgggcgaaca gatgggttag cccctacaaa
     1621 taatttgata tctcacgctt aaagtttaga atgtcatgga ttggagattt aaaattggag
     1681 gtattaaatg gatattgaga agttggtaaa tctttttcag gatactcaca atgagttgca
     1741 atcaagagcg gcaagaagtg ttgacacata tcttgttgtc agaaactggc ttttcggatg
     1801 gtatattgtt gagtttgaaa atggcggagc cgaacgagct gagttgtatg gaaaggagct
     1861 gattaaaaag ctctcggaaa aactcactgc aaatcttggc aaagggtttt caaaacgatc
     1921 tcttgaacag tttcgacaat tttacttaac ttataaagag attacgcagg cactgcctgc
     1981 acaatatgaa aaaagagcaa atgcaattag tccgacagtg tcggacaaat gtgaaatagt
     2041 ccatgaattt gagatgttgc ataatgtcca gacggtgtct gggatttctg agaatttaat
     2101 ttccgagaca gtgcctcgga tttcaaaaag cgattctctc gttgaactta ctctgccgaa
     2161 agacagcaat atttacgcat ccagttacca gctctatctt ccaagcaaag aagaactgaa
     2221 aaagcagatt gaagaagctg aaagagaatt ttcaaaaaat aaataaataa atttacacgg
     2281 gcgcgacaaa tcgtgcccct accattcaca catcacctgt cctgagttca tcgaaggacg
     2341 catcacgatt cacgcttcac gcttatcaaa ttatcttcaa cccattgaat gtatttatca
     2401 aatccgccga taacaggaat agtgattatt tcaggaactt cgtaagggtg tctctctttt
     2461 atgaactgaa ctgccgaatc ctgcagtgaa catgaagtct taacaaatgc aagtacctca
     2521 tcttcctgac atattttctc ttcccactca taaatgctta aaacaggagc agttatctga
     2581 atacacgaag caattctttt ttcgatcatc tcaagggcaa ttttccttgc cacctctttt
     2641 gaagggaatg ctgacacgat catcaccatt ttagtttcca t
//
LOCUS       DAOOYB010000353         2664 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_47550_length_2664_cov_7.376773, whole genome shotgun sequence.
ACCESSION   DAOOYB010000353 DAOOYB010000000
VERSION     DAOOYB010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47550_length_2664_cov_7.376773"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1110)
                     /locus_tag="PL056_13310"
     CDS             complement(1..1110)
                     /locus_tag="PL056_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HPM48039.1"
                     /translation="MDFQIVELLQSILIVSGIATVLAILMVIADATIGNYGIMNLLIN
                     KEHKHSVEGGKTLLNTLKEEGVFIPSACGGRGSCGLCKCKVTSGGGDYLPTELSFITK
                     DEQKEGIRLSCQIKVKRDFEIEIPEELFNVKEFKAKVASIKDLTHDIKEVRFQLLEPN
                     EISFKPGQFVQVQVPEYEFSNESVYRAYSISSSSTEKGIVELEIRYVNNGICTTYVHK
                     YLKEGDILTLTGPYGEFYLRDTKTDVVCVAGGSGMAPIKSILHHIEEQKISRKVTYIF
                     GAQGRRDLFLLERMKEFEKNIPDFRFVPALSNASLDDKWEGETGKVTEVLAKYLSDGS
                     NTEAYLCGSPGMINAVIEVLKSKGVKEENIYYDKF"
     gene            complement(1125..1724)
                     /locus_tag="PL056_13315"
     CDS             complement(1125..1724)
                     /locus_tag="PL056_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HPM48040.1"
                     /translation="MNGTETLGVFAIFFAAAITNNILLTMYLGMCSFLAVSRQVKTAI
                     GLGTAVVFVMTFTSAINWLIYEYLLIPFELEYLTYIVFIIVIAAFVQLVEMIIERVSE
                     TLYNALGIFLPLITVNCAILGAALFMIIRKYTLLSAISFSAGSGAGWMLAIIIMAGIR
                     QKMGTSKVPKGLEGAGITLIIAGIMSMAFMGFTGMMNLN"
     gene            complement(1721..2332)
                     /locus_tag="PL056_13320"
     CDS             complement(1721..2332)
                     /locus_tag="PL056_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HPM48041.1"
                     /translation="MTSKAGKITLANLGRENPVFAQILGICSTLAVTNVLENTAVMCA
                     GLIFTTALSNTTVSVLRKWIPSRIRMMITTLIIAFYVILVDIVLKAYLPDISRQLGAY
                     IGLIITNCIIMGRAEAFALNNKPGLSFVDGLTSGLGYGYVLLIIAFFRELLGSGTIWG
                     FTVLGDWWVKWSIMVMAPGAFFMLAVFIWIVKGLILVDPEVKK"
     gene            complement(2329..>2664)
                     /locus_tag="PL056_13325"
     CDS             complement(2329..>2664)
                     /locus_tag="PL056_13325"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HPM48042.1"
                     /translation="VAVFQSSGQGLWGVIKGHVGLKSDLETIAGIDFTVQTETPGLGA
                     RITEQWFKEQFRGKKGELKRVPEGTKSGNENEFDAITGATVTSKAVESMINKTVKNAP
                     AVAGKGVEQ"
BASE COUNT          736 a          648 c          481 g          799 t
ORIGIN      
        1 ttaaaactta tcgtaataga tattttcctc tttcacccct tttgacttga gcacttcaat
       61 gacggcattt atcattcctg ggcttccgca aagatatgcc tcagtgttcg atccgtcaga
      121 aagatatttt gcaagcactt cagtcacttt gccggtttca ccttcccatt tgtcatccaa
      181 agatgcattg cttaaagccg gaacaaatct gaaatccgga atattcttct caaactcctt
      241 cattctttca agaaggaaaa ggtctcttct tccctgagct ccgaaaatgt aagtcacttt
      301 tctacttatc ttctgttctt caatgtgatg cagtatcgat tttatcggag ccattcctga
      361 accgcccgca acacagacaa catcagtttt agtatctctt aaataaaatt ctccatatgg
      421 gccggtgaga gtaagaatat ctccttcttt caaatactta tggacatagg tcgtgcatat
      481 tccgttattg acatatctga tctcaagctc aactatccct ttttcagttg atgatgatga
      541 gatcgaatag gccctgtaaa ccgattcatt tgaaaattca tattcgggaa cctgtacctg
      601 aacgaactgt cccggcttga aacttatttc attcggctca agaagttgaa aacgaacctc
      661 tttaatgtcg tgcgtaaggt ctttaattga tgcaactttc gccttaaatt ccttcacatt
      721 gaaaagctct tcagggatct cgatctcaaa atctcttttc actttgatct gacaactgag
      781 ccggatacct tccttctgct catcttttgt gataaatgaa agttctgtcg gaagataatc
      841 gcctcctcct gaagtcactt tacatttgca aagtccgcat gagccccttc cgccacaggc
      901 cgacggaatg aaaacaccct cttctttgag ggtattaagc agtgtttttc ccccttccac
      961 gctgtgtttg tgctctttat ttatgaggag gttcattatt ccgtaattgc cgatcgttgc
     1021 atcagcgatc accattagaa ttgcaagcac tgtggctatt cctgaaacga tcagaatgct
     1081 ctgcagcagt tcaacaattt gaaaatccat tgccgccctc ctgatcagtt taagttcatc
     1141 attccggtaa aacccatgaa ggccatggac attattccgg cgatgataag tgttattcct
     1201 gctccttcaa gtcctttcgg aactttggat gtccccattt tctgcctgat acctgccatg
     1261 attattattg caagcatcca gccggcaccg cttcctgcac tgaaggaaat tgcagaaaga
     1321 agtgtatatt ttctgattat cataaaaagt gcggctccga gtatcgcaca gttcaccgtt
     1381 atgagcggaa gaaatattcc aagagcgtta taaagcgttt cgctaactct ttcaataatc
     1441 atttcaacaa gttgaacaaa tgcggcaatg actatgataa aaacaatgta agtcagatat
     1501 tcaagctcaa acgggataag aagatattcg tatataagcc agtttatcgc acttgtgaaa
     1561 gtcatgacaa aaacaactgc cgtgccaagt cctatcgcgg ttttaacctg tcttgaaaca
     1621 gcaaggaagg agcacattcc aagatacatt gtgagcagaa tgttgtttgt aatggcagcg
     1681 gcaaaaaata ttgcaaatac accgagtgtt tcagttccgt tcattttttc acctccggat
     1741 caacaagaat gagccctttt acgatccaga tgaaaaccgc aagcatgaaa aaggcaccgg
     1801 gagccatcac catgatcgac catttaaccc accagtcgcc aagaactgta aatccccaga
     1861 tcgttccgct cccgaggagc tctctgaaaa acgcaataat taaaagcaca tagccgtatc
     1921 caagcccgct tgtcaacccg tcaacgaaac tgagacccgg tttattgttc aaagcaaatg
     1981 cctccgctct gcccatgatg atgcagttag ttatgataag tccgatatat gcaccaagct
     2041 gtcttgaaat atcagggaga taagctttta gaactatgtc aaccagaatg acatagaaag
     2101 cgattatcag agttgttatc atcatcctta tccttgacgg gatccatttg cgcaaaactg
     2161 aaacagtagt atttgacaat gcggttgtga atatcaatcc ggcacacatt accgctgtat
     2221 tttcaagcac attcgtgact gctaatgttg agcagatccc gagtatctgg gcaaaaacag
     2281 gattttcccg ccctagattg gctaaagtta tctttcctgc ttttgaagtc attgttccac
     2341 ccccttcccg gcaacagcag gagcattttt aacggtttta tttatcattg attcaaccgc
     2401 ttttgaggtc acggtcgcac ctgttatcgc atcaaattca ttttcattac cactttttgt
     2461 cccttccgga acccttttta attcaccttt tttgcctctg aactgctcct taaaccattg
     2521 ctctgttatc cttgcaccga gccccggagt ttcagtctga actgtaaaat ctatccctgc
     2581 aattgtttca agatcgcttt taagaccgac atgcccttta attactcccc acagtccctg
     2641 tccgctgctc tgaaacactg caac
//
LOCUS       DAOOYB010000354         2663 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_47581_length_2663_cov_7.213957, whole genome shotgun sequence.
ACCESSION   DAOOYB010000354 DAOOYB010000000
VERSION     DAOOYB010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47581_length_2663_cov_7.213957"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(230..1714)
                     /locus_tag="PL056_13330"
     CDS             complement(230..1714)
                     /locus_tag="PL056_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020003948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPM48043.1"
                     /translation="METFTAEKLSSYIEALRPIIYINNFDFEAVDNLIRKASDGAKIF
                     EFNNVGEICFDGKHLKKDIDILAFLSAFDSDIKLHPSLLILKDIHNHLENPKVIASLK
                     SIAFKRMYQDDFNVTIFIVSTRLVIPVELEKLITIFDIPLPDQDEIEKIIRDYVKSFK
                     MNIKDKVVDELKLSFKGLSAFEIEQILNLAYQESGEIDEKDKDLILKEKEQNVRKSGM
                     LEIISVKDSLDEIGGLDGMKQYLEKKSDIFMNLSESKKFGVDLPKGILIVGMPGCGKS
                     LTAKVTSKMFDVPLLRLDVGKLMGKYVGESEDNLRRAIKTAEAVAPCVLWIDELEKAF
                     AGVGGSGGGSDVTTRLFGNFLTWLQEKESSVYVVATSNDISKLPPEFLRKGRFDELFF
                     VDLPNDEERRQIFEIHLKKRRKMNKNIDTIKLLKSTAGYSGADIESVVKEAIEEAFCD
                     GKREVDTNHMLEIIKKTKSISKTLKEKIDELKKVLEKYDMKPAS"
     gene            complement(1717..>2663)
                     /locus_tag="PL056_13335"
     CDS             complement(1717..>2663)
                     /locus_tag="PL056_13335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48044.1"
                     /translation="SGVLQRWLQVRDYKECLKKVIEINNQDKIRIAYELVRIFDATKT
                     DEEIKNSLYYLEFRKNKEEQLKHLEKNKFNCIEVVADHYANYKNVLNMIIEKKDDFAY
                     IKQSVKEIETNYLELFEFSHMAFYEKMTKNAPLAIFAALMNESLRNYFIYNPELDKLP
                     DVSATKKAFNLFIKDFFSFQYSSSPHYNKVFIDKVECFMKQFTEQPGSSTINTMLREL
                     GQIGAENSKEVIHTWYDTVAKYLKYVVCTTNDHWKDIEPTGKRYLVLFVGRNCMVRNA
                     GRHGEELKENDVLLKYPILSGIDYKCNTPQVPLIYMEV"
BASE COUNT          778 a          515 c          407 g          963 t
ORIGIN      
        1 agtgcggcgg actcgacgaa tacatcaaag caaaactcgc cgggaaatag ttaaataaac
       61 cttattttct taatcatcat cccctcatgg aagtggcttt tggggagtta gagaaatcat
      121 tttttttgac agtgcaaagc ttccggtatg ttagtttttc catatactta ctggttggga
      181 gttaagagaa aaagctaaac agatctgtgg gtgtaaaaaa tatgtcttat caggacgcag
      241 gtttcatatc gtatttctca agaacctttt tcagttcgtc tattttttct tttaatgttt
      301 tggatataga ttttgttttc ttaatgattt caagcatatg atttgtgtca acttctcttt
      361 ttccatcaca aaaagcttct tcaatggctt cttttacaac ggattcaata tcagcgccac
      421 tataacctgc tgtggatttt agaagcttta ttgtatcaat gtttttgttc atttttcttc
      481 gttttttaag atgaatttca aatatctgtc ttctttcctc atcatttggc agatcaacaa
      541 agaaaagctc atcaaatcgc cccttcctta aaaattccgg gggtaattta gatatgtcat
      601 ttgaagttgc aacaacatat actgagcttt ccttttcctg aagccatgta agaaaattgc
      661 cgaaaagacg agttgtaaca tcacttccgc ctccgcttcc tccaactcca gcaaatgctt
      721 tctcaagttc atctatccaa agaacacacg gagctaccgc ttcggctgtt tttattgctc
      781 ttctcagatt gtcttcactt tcccccacat attttcccat aagttttccg acatcaaggc
      841 gaagaagcgg aacatcaaac atttttgaag taacttttgc ggtaagagac tttccacaac
      901 ccggcattcc tacaatcaga ataccttttg gcagatcaac cccaaatttt ttggattcgc
      961 ttaagttcat aaaaatatca gatttttttt ccagatactg cttcatgccg tccaggccac
     1021 ctatttcatc aagagaatct ttaacgctta tgatctcaag cattccgctt tttctgacat
     1081 tctgttcttt ttcctttaaa ataagatcct tgtctttttc atcaatttct ccggattcct
     1141 gatatgcaag atttagaatt tgttcaattt caaaagcact caacccttta aaagagagtt
     1201 tcagttcatc aactactttg tctttaatat tcattttaaa tgattttaca taatctctga
     1261 taattttttc aatttcatcc tgatcaggta gaggaatatc aaatattgtg atcagtttct
     1321 caagttcgac tggaattaca agacgagttg aaacaataaa aatggtaaca ttaaaatcat
     1381 cctgatacat tcttttaaat gcgatagatt tcaagctggc aatgactttt ggattttcca
     1441 aatgattatg gatgtctttc aaaatcaaaa gagacgggtg caacttaatg tccgagtcaa
     1501 atgctgataa aaacgcaaga atatcaatat cttttttgag gtgttttccg tcaaagcata
     1561 tttctccaac attattaaat tcaaaaattt ttgctccgtc ggatgcttta cggattaaat
     1621 tatccactgc ctcaaaatcg aagttattta tgtagattat cggtcttaat gcctcaatgt
     1681 atgaggaaag tttttcggcg gtaaaggttt ccatttctaa acctccatat aaattagcgg
     1741 aacctgaggg gtgttacatt tataatcaat gccacttaat attgggtatt ttagcaacac
     1801 atcgttttct tttagttcct ccccgtgccg cccagcattt ctcaccatgc agtttcttcc
     1861 aacaaacaac actagatatc gcttaccagt tggctcaata tctttccagt gatcatttgt
     1921 agtgcaaaca acatatttta agtattttgc aacggtatcg taccaagtat gaataacttc
     1981 tttagagttt tctgcaccta tctgacccag ttccctgagc attgtgttta tggttgaaga
     2041 ccctggctgc tcagtgaact gcttcataaa acactctact ttgtcaataa aaaccttgtt
     2101 gtaatgtgga gaagaggaat actgaaaaga aaagaaatct tttataaaaa gattgaatgc
     2161 cttttttgtt gcagaaacat ctggcaattt atccaactca gggttgtaaa taaaataatt
     2221 tcttagagat tcattcataa gtgccgcaaa tattgccaga ggagcatttt ttgtcatctt
     2281 ttcataaaaa gccatgtggg aaaactcaaa taattccaaa tagtttgtct caatctcttt
     2341 tacagattgt tttatatagg caaagtcatc ttttttttca attatcatgt tcaagacatt
     2401 tttatagttt gcgtaatgat ctgcaacaac ttcgatacaa ttaaatttgt tcttttcgag
     2461 gtgttttagt tgttcttctt tattttttct aaactccaaa taatacagtg agttttttat
     2521 ttcttcatct gtctttgtag catcaaatat ccgcacaagc tcataagcaa ttcttatttt
     2581 atcttgattg tttatttcta ttactttttt aagacactct ttgtaatctc ttacttgaag
     2641 ccatctctga agaactccac ttt
//
LOCUS       DAOOYB010000355         2643 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_48054_length_2643_cov_6.984544, whole genome shotgun sequence.
ACCESSION   DAOOYB010000355 DAOOYB010000000
VERSION     DAOOYB010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48054_length_2643_cov_6.984544"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1198)
                     /locus_tag="PL056_13340"
     CDS             complement(<1..1198)
                     /locus_tag="PL056_13340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48045.1"
                     /translation="MLNRKLYGFLLIIFFTTAACNSDKPKNDVDINDKDSGLTDIDTP
                     VQDPDTVEIDTEQPDIDADADTNVPCLDLRYNENTIKTNFPFKDKNEKPTFCRPGCDI
                     PTETDPQCVRNIWEWDNWSEYQVYLEAEKKNPNQELERECFPWPCKLPDMKANSTLES
                     FNSKCDRWLTVKGFKASEGTIWSHGMSDGVAGMDFGHTGRAIEYDPEKDEYSTLGQNR
                     YLSFNEGRYVIEVYDRVPGDNVGTYKVFTVSIMKKDEKYYYEFIYDSGSAYEEFTGPA
                     FAGEKWVLIQIGNRTTGKESFKYASSKNWQWHNIEGIQNYAGEGNIVGDHLTFITNNR
                     EIYYCDLRKYPKHINDCKEINRLNTDGITYELGHSPMIDSENENRLVYNVYGENKFVE
                     VILTDID"
     gene            complement(1202..>2643)
                     /locus_tag="PL056_13345"
     CDS             complement(1202..>2643)
                     /locus_tag="PL056_13345"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HPM48046.1"
                     /translation="HEVKIEEAIAEIEIFHDNERKYFMTFASRPLKIKQCIEVNYDND
                     PVELRNEISSHAKREVEILQELLDISSRKEDLYKYYLEELNKVNFNKAAGGSVHEFNG
                     RIFLLQAWCPLKDLEELKRTLENETVTIIPVDPEKDERIPTLIDPKKTMDELGIDLVN
                     IYDTPSYKDWDPSSWVFFAFTIFFAMIMADGGYGLLLLAFMLFLKIKVKKPSPSIKRF
                     INLSIVLSSATSVYGLISGGFFGMSLDTPAFTFLAPFTDLLKSIRFINSNDTQMMMLV
                     SIMIGMVHIGISLILKGLRALIDEKDYVTPLINLTWIAGIWSFFFWYKYEGVAGAQHL
                     TSGGLIGLKIVGGVLVVLYAIAAKTLNPLKMFMSSFFGLYNGIQFFSDILSYIRIFAL
                     GLSGALLAQTFNNLSFDLWNAGIGGMIIAPVIFLLGHTLNITLCIMGGVIHGLRLNFL
                     EWYRWSFDGGGKRFVPFKDLLKAYVKSES"
BASE COUNT          748 a          571 c          481 g          843 t
ORIGIN      
        1 tatcaatgtc cgtcagaata acttccacaa atttattctc tccatataca ttataaacca
       61 gccggttttc attctcggaa tctatcatcg gtgaatgccc gagttcataa gttattccgt
      121 cagtgttcag acggtttatt tctttacagt cgttgatgtg ttttggatac tttctcaggt
      181 cacagtaata tatttcacgg ttgtttgtga tgaatgtcag gtgatccccc acaatgtttc
      241 cttctccggc ataattctgt attccttcaa tattatgcca ctgccaattc tttgaggagg
      301 catatttgaa tgattctttt ccagttgttc tgtttccgat ctgtataaga acccatttct
      361 ccccggcaaa agcaggtcct gtgaactctt cataagctga gccactgtca taaatgaatt
      421 cgtaataata tttttcatcc tttttcatga tagaaacagt aaaaacttta tatgttccga
      481 cattatcacc cggcacacga tcataaactt cgataacata tctgccttca ttgaaagata
      541 aatatctgtt ctgacccaat gtcgagtatt catctttttc agggtcgtat tctattgctc
      601 ttccggtatg tccaaagtcc attcctgcaa caccatcact cattccatgt gaccagatag
      661 ttccttcgct tgctttgaac cctttaaccg taagccacct gtcacacttg ctgttaaatg
      721 attcaagggt tgagtttgct ttcatatcag gcagtttaca tggccatgga aaacactctc
      781 tttccaattc ctgatttggg tttttctttt ccgcttcaag ataaacctga tattcgctcc
      841 agttgtccca ttcccagata tttctaacac actggggatc agtttcagtc ggtatgtcac
      901 atccgggacg gcagaatgtc ggcttttcgt tcttgtcttt gaaggggaaa tttgtcttga
      961 tcgtgttttc gttgtatctg agatcaagac atggaacatt ggtgtcagca tcagcatcaa
     1021 tatccggctg ttcagtatca atttcaacag tatcgggatc ttgaacagga gtgtcaatat
     1081 ctgtaagtcc tgaatccttg tcattaatgt caacatcatt tttcggctta tctgaattgc
     1141 aggcagcggt tgtgaaaaaa attattaaca aaaaaccata tagttttcta tttaacatcc
     1201 actaactctc tgatttcaca tatgctttaa gcagatcctt aaacgggacg aatctctttc
     1261 cgcctccgtc aaaagaccac ctgtaccatt caagaaaatt aagtcgcagt ccgtggatga
     1321 ctccacccat tatgcaaaga gtaatgttaa gtgtatgtcc aagcagaaaa atgacagggg
     1381 cgattatcat tccgccgatt ccagcattcc acagatcgaa tgaaagattg ttaaatgtct
     1441 gggcaagaag tgctcccgaa agtccgagag caaaaattct gatataactt aaaatatcac
     1501 tgaaaaactg aattccgttg taaaggccga aaaacgaaga catgaacatt ttcaacgggt
     1561 taagtgtctt tgccgcaatt gcatataaaa caacaagcac tcctccgacg attttcaatc
     1621 cgatcagtcc gcctgaagta agatgctgtg ctcctgcaac accttcatat ttgtaccaga
     1681 agaaaaatga ccagataccg gcgatccaag tcagatttat aagcggagta acataatcct
     1741 tttcatcaat aagggctctc aaacccttca atatcagtga aataccgatg tgaaccattc
     1801 ctatcattat tgaaacaagc atcatcatct gtgtgtcgtt agagtttatg aacctgatgc
     1861 ttttgagcag atctgtaaaa ggggcaagaa aggtgaatgc cggagtatca agagacatcc
     1921 cgaaaaatcc tcctgaaatc agtccataga cagatgttgc actgcttaac actatggaaa
     1981 gattgatgaa tctctttatt gacggagatg gtttcttcac ctttatcttt agaaacagca
     2041 tgaatgcaag aagaagcagt ccgtatccgc cgtcagccat tatcattgca aagaaaatgg
     2101 tgaatgcgaa aaacacccat gaactcgggt cccagtcttt gtatgaaggg gtatcgtaaa
     2161 tattgacaag atctattccc agttcatcca tagttttctt tggatcaata agggtcggaa
     2221 ttctttcatc tttctcggga tccacaggta tgatggtcac tgtttcgttc tcaagtgtcc
     2281 ttttgagctc ttcaagatct tttaaaggac accatgcctg aagaaggaat atcctgccgt
     2341 tgaattcgtg gacagatcct cctgccgctt tgttgaaatt aaccttgtta agctcttcaa
     2401 gataatattt ataaagatct tcctttcttg aagagatatc aagaagctcc tgaagtatct
     2461 ctacttctct ttttgcgtgt gatgatatct cgtttcttaa ttctaccgga tcgttatcgt
     2521 aattaacttc gatacactgc tttatcttta gaggtcttga ggcaaaggtc ataaaatatt
     2581 tccgctcgtt gtcgtgaaat atctcaattt cagcaatagc ttcttcaatt tttacctcat
     2641 ggg
//
LOCUS       DAOOYB010000356         2633 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_48332_length_2633_cov_5.676881, whole genome shotgun sequence.
ACCESSION   DAOOYB010000356 DAOOYB010000000
VERSION     DAOOYB010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48332_length_2633_cov_5.676881"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="PL056_13350"
     CDS             complement(<1..386)
                     /locus_tag="PL056_13350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48047.1"
                     /translation="MKQFRKIAAFLMVLVMLNFSLHASIFGEENATLSAILTEEVIQV
                     FYLAETITQLKAILDAGNQTIDLARESYRQFQKIKNYTWQDLLSDAKAGFCKGLDSTT
                     DTNCSDWESSLNEMKDNYDMIKGGEG"
     gene            complement(411..959)
                     /locus_tag="PL056_13355"
     CDS             complement(411..959)
                     /locus_tag="PL056_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016077325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HPM48048.1"
                     /translation="MNTVDPIREISDIQKMKSVLLNSNYRDYLIFTLGINTGLRISDI
                     LNLKVSDVRGKYHIIIREQKTGKEKKFLINDLLKEELERFIASKEDEEFLFQSKRNRN
                     KPLDRQRIYFILNVAAEEAGLKIRIGTHTLRKTFGFHFYQRTKDIALLQQLFNHSGQS
                     VTLRYIGINQSISDQAMRTFNL"
     gene            complement(2025..>2633)
                     /locus_tag="PL056_13360"
     CDS             complement(2025..>2633)
                     /locus_tag="PL056_13360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48049.1"
                     /translation="IWASQNWPTWKNKDGEFLGHIRTSRYKILNLILQKNPGSNDYKS
                     LMDTLYELKVIPVEVEDLVSDETREVFSIFFGFDFESEKEKDVISIYLDPKITKQYYR
                     KQDLKLLFFDTYRNLSSDISKTLYPIIDRALAENSEFSKKVIDLCNENGLTIYRYNSD
                     YRAKWKKAFAELNQITLTNGRKISVEFYENKLKELILLAKSF"
BASE COUNT          790 a          511 c          498 g          834 t
ORIGIN      
        1 tgaccttctc cgcctttgat catatcataa ttatccttca tttcattaag acttgactcc
       61 cagtctgaac agttagtatc tgttgtgctg tcgagtcctt tacaaaatcc cgcctttgca
      121 tcggaaagaa gatcctgcca tgtataattc tttattttct gaaactggcg gtaactctct
      181 cttgcaagat caattgtctg gttcccggca tcaagaattg ccttaagttg tgtaatggtt
      241 tcagccagat aaaacacctg aatcacttct tcagtgagaa ttgccgaaag cgttgcgttc
      301 tcctctccga aaattgaagc gtgaagggaa aaattcaaca tcacaagaac catcaaaaaa
      361 gccgcaattt ttctgaattg tttcatgatt aaaacctcta tttgtttttt ttacaaatta
      421 aatgttctca tagcttgatc ggatatactc tgatttatgc ctatataccg caaagtaaca
      481 ctctggccgg aatgattgaa aagctgttgc aataatgcaa tgtcttttgt tctctgatag
      541 aaatgaaaac cgaaagtctt tctgagcgtg tgcgttccta tacggatttt caaaccggct
      601 tcttcagcag cgacatttaa gatgaaatat attctctgtc tgtcaagcgg tttatttcta
      661 ttccttttcg attgaaaaag aaattcttca tcttccttgg aagcaataaa tctttcaagt
      721 tcctctttca ggagatcatt tattagaaat tttttctctt tgcctgtttt ctgttccctg
      781 ataataatgt gatatttacc tctgacatcg gaaactttaa gattaagaat gtccgagatc
      841 ctgagtccgg tatttatccc gagcgtaaaa atcagatagt ctctgtaatt tgaattcagg
      901 agaacagatt tcattttctg aatatcactg atttcacgga tggggtcaac tgtgttcata
      961 actctcaata aaaggttaat aacactacaa aatacaacgg tatgtagtga aagtcaagat
     1021 aaaaaattac cggctttcag acaattcaga cagttgtgga tttaacacga cagaatatgc
     1081 attgtgtcgt gttattttcc ggacggaaaa attctttcgt tttaagagct ttaaatcttc
     1141 tcgttcggga aagtaccctc gatgaacccc gaaaatgcgt taaaagtgaa atgtggtctt
     1201 tgagggtata aaagaaagct cattttcaat cgtaagcggt aagatttgaa gatgaacggc
     1261 atttgttctt ataataatgt tttgaagggc gtacgcttat attactgatc tgaaggaatg
     1321 taagcagtag cccggcagat ctgaacggta tattttgcga caaggcgtaa tccccgagcc
     1381 agtcgagggg tgcccgcaaa atatcctctt gttctgatag atagcgttac cacgattttt
     1441 gaatttataa cgggtttaag cgttaaagat atttttgcat tggcgtaaat ccttgaagcg
     1501 agcttgcgaa gcggaaaggt gcgcaaaaat atccttattt ggggggttcg gggggaaatt
     1561 tgaaatttta cggtacagat gacacccctt ttcttttata ctttagaaat actatgaaaa
     1621 tactatagca aaaatcagga aggggcgaag taaccagaat aattcaataa attgaacata
     1681 aaaaaaggcc ggattacacc caattcccga tagtgctaca ctcaattccc gatagtacca
     1741 cactcaattc ccgatagtac tacacccaat tcccgatagt actacaccca attcccgata
     1801 gtgctacact cagttcccga tagtgaatac cgtggggaat ttgtaccaaa tgtttaaaga
     1861 acgggttcat caaccccaga aatggaactt aaaaacgaaa aatagcggga aacggcggga
     1921 tcacaaccgt ataatggaca aaaatgtcag ggggataaaa aaatatgccg catttactac
     1981 acccaattcc cgatagtgct acactcaatt cccgatagtt tgaattagaa ggatttagcc
     2041 agtaaaatca attccttaag cttgttttca taaaattcca cagatatttt tctgccgttt
     2101 gtaagtgtta tctggttcag ttcggcaaag gcttttttcc attttgctct gtaatctgaa
     2161 ttatacctgt aaattgtaag cccgttctca ttacagagat ctatgacttt ttttgaaaat
     2221 tcactgtttt ctgcaagtgc acggtcaatt atcggataaa gcgtttttga tatatcactg
     2281 gaaaggttgc ggtaagtatc aaagaaaagg agtttgagat cctgcttgcg gtaatactgc
     2341 tttgttatct tcgggtcgag atatattgag ataacatctt tttccttttc agactcaaaa
     2401 tcaaaaccga aaaagattga aaagacctct cttgtttcat ccgagaccag atcttcaact
     2461 tcaacaggga tcaccttcaa ttcataaagt gtgtccatta gactcttgta atcattggag
     2521 ccgggattct tctgaagtat gaggtttaat atcttatatc tgctcgttct tatgtggccg
     2581 agaaactcac cgtccttgtt tttccatgtc ggccagttct ggcttgccca tat
//
LOCUS       DAOOYB010000357         2628 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_48470_length_2628_cov_9.559658, whole genome shotgun sequence.
ACCESSION   DAOOYB010000357 DAOOYB010000000
VERSION     DAOOYB010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48470_length_2628_cov_9.559658"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            122..2173
                     /locus_tag="PL056_13365"
     CDS             122..2173
                     /locus_tag="PL056_13365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48050.1"
                     /translation="MRNLSIFTFFLLFLFQTSCGLSTSDSNVDNDTVIADIDGDSDID
                     IETEENEFETEKNESETDENNFDKNAYIEPRFIFDIFFESAPHGITRWDEKFIYHIKC
                     SSAQGLETSLKVSSEDTCGGTVSSNIYSFAPVKEKFPEKKCNLSVTCYNKKDSRTQTT
                     VLLIPNPDILMPELNNNGHEFNFVSSSAEKDQFTITKVPPQNDYVTPSEINVYEYYKN
                     SKKIEPLSKISALKAKVLYSGKETALIFTDYYVFLYDIKTQTKTELAYNPGTHETVQT
                     GPMQTATIYYPGIVSFNKTIGNVTFFGVSSGIDWYSLSTTDGTQEGTSGHELVVSSIV
                     RYGNTLDAVTKILADKYGGFYFNDTYYYHYVENSGTPSFKTDAFLPDTAGFDGERMYY
                     EKQLGNRNKLVSLKKGKSDLEYIAPDYRSVNDGHIVNGNLIIIEQGEEGYISMFKNCD
                     IAKEPVRLKIFDNYTYKWTRVEAIGNNYAVISCFRKNENITDLYKVVFDSNRLISISG
                     PMGYIHNIDPTERLENAFLVDKFFVYEKDDSLFSIDSNAIDYYPVKLETIATTKDITD
                     LSFNFLGKDISNEAVISYYKPDDDKKYIAKTDGTKDNYKKIEYPSYTDVNVWNEFILE
                     NNSHFIIEESGELVRKQICNESFFNTHQYKTDKAIYFIDKTDERSQKFFIFRMPETVL
                     K"
     gene            complement(2200..2451)
                     /locus_tag="PL056_13370"
     CDS             complement(2200..2451)
                     /locus_tag="PL056_13370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48051.1"
                     /translation="MKKGIIFLVILAASSALFANKVQCERVGNSSSDIFKCSAGKGSI
                     VYHIDKVTGICFAGIDGIPESFTLVDCSQVLKRSEIAIP"
BASE COUNT          938 a          483 c          443 g          764 t
ORIGIN      
        1 aaaaacagca ttattatagt aatttagaca tttttatgca aatctgtcgg gatttgacaa
       61 aaaagaaact ttcagataaa ttattgtgtt acagtaagtg acttttttct aaggtgcaac
      121 tttgaggaat ctttcaattt tcacattctt tttacttttt ttatttcaaa catcctgtgg
      181 attatcgact tctgacagca atgttgacaa cgatactgtt attgcggata ttgacggcga
      241 cagtgacatt gatatcgaaa cagaagaaaa cgaatttgag acagaaaaaa atgaatctga
      301 aaccgacgaa aataatttcg ataaaaatgc ttatatagag ccaagattta tattcgacat
      361 attttttgaa tccgcaccgc atggtatcac cagatgggat gaaaaattta tttaccacat
      421 aaaatgcagc tcagcacaag gacttgaaac atcattaaaa gtttcatctg aagacacctg
      481 cggcggtact gtcagctcca atatttacag ttttgcccct gttaaagaaa agtttcctga
      541 gaaaaaatgc aacctttctg taacatgcta caacaaaaag gattccagga cacagacaac
      601 agttctttta attcccaatc ctgacatttt aatgcctgaa ctgaataata acgggcatga
      661 atttaatttt gtatcgagtt cagctgaaaa agatcagttt acaataacta aagttcctcc
      721 acagaacgat tatgtaaccc cttcagaaat aaatgtgtac gaatattaca aaaattcaaa
      781 aaaaatagaa cccctttcta aaatcagtgc attaaaagca aaagtgcttt attccgggaa
      841 agagactgct ttgatattca ccgattatta tgtgtttctg tacgacatta agacacagac
      901 aaaaacagaa ctggcttata atccaggaac ccatgaaaca gtacagacag ggccgatgca
      961 gacagccaca atttactatc ctggaattgt cagctttaac aaaacgatcg gaaatgtgac
     1021 atttttcgga gtatcttcag gaattgactg gtacagtctt tccactaccg acggaacaca
     1081 ggagggaact tccggacatg aactggtagt aagctcaata gtcagatatg gcaacactct
     1141 ggatgctgta acaaaaatac ttgcggacaa atatggcgga ttttatttta acgataccta
     1201 ttattatcac tatgtcgaaa acagcggaac accgtcattt aaaactgatg cttttctacc
     1261 tgatactgcc gggtttgacg gtgaacggat gtattatgaa aaacaactcg gcaaccgcaa
     1321 caagcttgta tcattgaaaa aaggaaaaag cgatctggaa tatattgctc ccgactacag
     1381 aagtgtcaat gacggacata ttgtgaacgg aaatctgata ataattgaac agggtgagga
     1441 aggttatatt tcaatgttta agaactgcga catagcaaaa gaaccggtcc gtcttaaaat
     1501 ttttgataat tatacataca aatggacaag agttgaagcg atcgggaata attatgccgt
     1561 gatttcatgt tttagaaaaa atgaaaatat aacagatctt tacaaagttg tttttgacag
     1621 caatcgctta atttctattt caggtccaat gggttatatt cacaatattg atccaacaga
     1681 acgacttgaa aatgcatttc tagtcgacaa attttttgta tacgaaaaag atgattctct
     1741 tttctcaata gatagcaacg caatagatta ttatcccgta aaacttgaaa caattgcaac
     1801 aacaaaggac atcacagatc tgtcatttaa ttttttggga aaagatattt caaatgaagc
     1861 cgtcatttca tattataaac ctgatgatga caagaaatat atcgcaaaaa ctgacgggac
     1921 aaaagataat tacaaaaaaa ttgaatatcc atcatacacc gatgttaatg tctggaatga
     1981 atttatactt gaaaacaaca gtcatttcat aattgaagaa agcggagaac ttgtccgcaa
     2041 acaaatctgc aatgagtcgt ttttcaacac tcatcagtat aaaacggaca aagccattta
     2101 tttcattgat aaaaccgatg aaagatcaca aaaattcttt attttccgaa tgcctgaaac
     2161 agttttaaaa taaccatctc cgctggactt ccttaaaatc taaggaatcg cgatttcaga
     2221 gcgttttaaa acctgtgaac agtcaacaag agtgaacgac tcaggaattc cgtcaattcc
     2281 agcaaaacag atgccagtca ctttgtcgat gtgataaacg atcgaccctt ttcctgcact
     2341 gcatttgaag atgtcactgc ttgaattccc caccctttca cattgcactt tgtttgcaaa
     2401 aagagcactc gatgcagcaa gaattactaa aaaaattatc ccctttttca tggtctcctc
     2461 catagtttta attaacagga caattatctg acaacagaaa cgatgaattt gtaaaaaatt
     2521 tataactttt caaaaggttt cattatatct ttgccagcat cttcgtcact ttgacattca
     2581 ttttggaaaa accgaaccat tttttcccga gatctttgag tgatgcac
//
LOCUS       DAOOYB010000358         2601 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_49234_length_2601_cov_10.401414, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000358 DAOOYB010000000
VERSION     DAOOYB010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49234_length_2601_cov_10.401414"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..929
                     /locus_tag="PL056_13375"
     CDS             <1..929
                     /locus_tag="PL056_13375"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HPM48052.1"
                     /translation="QAIEETVKGSRRECVDEKCQLSLVAQLQANFLINTKIKKLYEGT
                     CNISISKFDVEKRAGVDSWTTDFNCSEKGVFEAINSFDFGGKKEQVGVLKQDDISDKN
                     ACKYAKEKNNLEIWEKYIEKYPKGECEFEATVKIEELNKKKSEKDENTKTSSGKIIPP
                     PKKKELKKPTDLNVKALFRWSKKASEEMTWNDAIKYCENLKEDGYSDWRLPTISELRT
                     LIKNCPATETGGECKVADSCLSYKNCRNDACKGCLSVSDGRYSKFGDTEWFWSSSEPL
                     DLDNYAWQLNFDNGRTSYSAKRYVSNVRCIKN"
     gene            944..1678
                     /locus_tag="PL056_13380"
     CDS             944..1678
                     /locus_tag="PL056_13380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48053.1"
                     /translation="MKFIKITTLFVLLIFSWESCFASFRKNVKAQYKNNSGWSDQFSV
                     EAIFMKGPELNKATNSYKYTSYSLYAVIFWNQEGNASVIELDGYDSCGLEADRDCIEY
                     HIGDWEGKDQQGREWTICSGHFCTAKSNSYSYVPNYSINNGNSTKSYNSNSKTRHWIN
                     AGLVIMLIGFIVLDVGLLLYIMDEEQETPGLITMAVGGGLMVLGPLFMLGDISSSSHH
                     SENNLYEKRKIFSGSKTLGLSLRFEF"
     gene            1697..2329
                     /locus_tag="PL056_13385"
     CDS             1697..2329
                     /locus_tag="PL056_13385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48054.1"
                     /translation="MKIFFLFGSGISIPAEIAKVQNITDSILCGRDYGDEIEHFEGRA
                     GNYLKKINKDQYLSTEEKEHIQNITEFLSIINDEANRFLEKRGFNRKANYEDLYYLTM
                     QFDHIFNPEAIQWKFKQMLEEADCLVISGYGFGDDTINNRIASWVLSNKNRKIILIHK
                     DPESLYDKADKIVDKGLWSELLSVKRLLIIAKWIQDVSWSEIKSHLPSSK"
BASE COUNT          951 a          354 c          535 g          761 t
ORIGIN      
        1 aacaggcgat tgaagaaacc gttaaaggat caagaagaga atgcgttgat gaaaagtgtc
       61 agttgtcact ggtggcacag cttcaggcaa atttcttaat aaatacaaag atcaagaagc
      121 tttatgaagg aacatgcaat attagcatca gcaaattcga tgttgaaaaa agagccggag
      181 tagattcatg gacgactgat ttcaattgtt ctgaaaaagg tgtttttgag gcaattaaca
      241 gttttgattt cggtggaaag aaggaacaag taggcgtttt aaagcaagat gacatatctg
      301 ataagaacgc ttgtaaatat gcgaaagaga aaaataattt agaaatttgg gaaaaatata
      361 ttgaaaaata cccgaaaggt gaatgtgaat ttgaagcaac ggtaaaaata gaagaactga
      421 acaaaaagaa atctgaaaaa gatgaaaaca caaaaacatc ttcaggcaaa atcattccgc
      481 ctccgaaaaa aaaggagctg aaaaagccaa ctgatctgaa tgtaaaagct ctgttcagat
      541 ggtcaaagaa agcctctgaa gaaatgactt ggaatgatgc aattaaatat tgtgaaaatt
      601 taaaagaaga tggatattca gattggcggc ttcctacaat atctgaactc agaacactta
      661 ttaaaaattg ccctgcaaca gaaacaggtg gcgagtgcaa agttgctgac agttgtcttt
      721 catataaaaa ttgcaggaat gatgcttgca agggttgttt aagtgtttcc gatggaagat
      781 attccaaatt tggagacaca gaatggttct ggtcatcatc ggagccactt gatcttgata
      841 actacgcctg gcagttaaat tttgataatg gtagaactag ctatagtgcc aagcgctacg
      901 tgagtaatgt tcggtgtata aagaattaag agaatagcat ttaatgaaat ttataaagat
      961 aacaacactc tttgttttat taatcttttc gtgggaaagc tgtttcgcat cttttagaaa
     1021 aaatgtaaaa gcacagtaca aaaataatag tggctggtct gatcaattta gcgttgaagc
     1081 aatatttatg aaaggacctg agcttaataa agcaacgaac tcttataaat atacatctta
     1141 ttctttatat gctgtgattt tctggaatca agaagggaat gcttcagtta tagagttaga
     1201 tggttatgat tcttgtggtc ttgaagcaga tagggattgc atagaatatc atattggaga
     1261 ctgggaaggg aaagatcagc aaggaagaga atggacaatt tgttcagggc atttctgtac
     1321 agcaaaatca aacagttata gttacgttcc aaattattca atcaacaatg gcaactcaac
     1381 taaatcgtat aactcaaact ccaaaacaag acattggatt aatgcggggc ttgtaatcat
     1441 gttgatagga ttcattgtgt tggatgtcgg acttttgctt tatataatgg atgaagagca
     1501 ggaaacccct ggattgataa caatggctgt aggtggtggg ttaatggtat taggaccact
     1561 ttttatgctt ggagatatta gttcaagcag ccatcattca gaaaacaatc tttatgagaa
     1621 aagaaagatt ttctcaggta gcaaaactct tggattgtct ttaaggtttg aattttaaat
     1681 taatgggaac aatagcttga aaatattttt tttatttgga tctggaattt ccattccagc
     1741 tgaaattgca aaagttcaaa acattacaga ttcaatactt tgcggaaggg attacggaga
     1801 tgagattgaa cattttgaag gaagagctgg gaattattta aagaaaataa acaaagatca
     1861 gtatttatca actgaagaaa aagaacatat tcaaaacatt acagaatttc tttccattat
     1921 aaatgatgaa gccaatcgat ttttggaaaa aagaggattt aatcgaaaag ctaattatga
     1981 agatttatac tacttgacga tgcagtttga tcatatattt aaccctgaag caattcaatg
     2041 gaaatttaaa cagatgctgg aagaagcaga ttgtcttgta atttcaggat atggattcgg
     2101 agatgacacc attaataata ggattgctag ctgggttctt tcaaacaaga atcgtaaaat
     2161 tattttaatt cataaagatc cggaaagctt atacgacaaa gccgataaga ttgttgataa
     2221 agggctgtgg tcagaattgt tatcagtcaa aagacttctt ataattgcta aatggattca
     2281 agatgtaagt tggtctgaaa taaagagtca tcttccttca tcaaaataac aactcccttc
     2341 tcaaattcca gcaatagggg tcagggagca atataaacat aggggtcagg gaacaatata
     2401 tttatatata aaaatgtgac tttagttgcc cccaacaccc taaatcaatt ccactttttt
     2461 cagttttttg gcgagtaaga aaattttcct gaatttcttg taaattaatc tggcattaga
     2521 tatattgact ctggacattg aatataagaa gcgatgaatt ttgtgtgtcg gagggtcgtg
     2581 ataaaaatga atgaaattgt a
//
LOCUS       DAOOYB010000359         2598 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_49307_length_2598_cov_13.720016, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000359 DAOOYB010000000
VERSION     DAOOYB010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49307_length_2598_cov_13.720016"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="PL056_13390"
     CDS             complement(<1..686)
                     /locus_tag="PL056_13390"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HPM48055.1"
                     /translation="MKEYIIPKNRFSGFYHFGEVSSTMDKAIEFIEKGVNRGIVVADL
                     QSGGYGRNGKSWFSPDNGNLYLSFFEKLDRSEPLNLIPQRIALSAQMAVKKFVDTGNI
                     SIKWPNDILADMNKCAGIIAKTVQRGVDRFYVCGIGINVFMPETTLFEHNWKVGALNE
                     YVPDITTEAVLAELIRTLDTFFEMNETQIKEKYVSEISWMTDRSITYTINGTDLETGT
                     VTGFSNNGSA"
     gene            838..2169
                     /locus_tag="PL056_13395"
     CDS             838..2169
                     /locus_tag="PL056_13395"
                     /inference="COORDINATES: protein motif:HMM:NF024598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HPM48056.1"
                     /translation="MHRFFIFISILFFSINAYSENSEVFVNADILNIRTYPTTNSAKT
                     VLAYRGDILIIFESKKDSEGKEWIKVALKKKSSEAVGWVLAEYTVKTRASLLNQIYKD
                     LDYSPQNKTDGYKNNPKVKVKGIYLTRYSSTPTRLNHFFNLVKGTEINAFVIDIKNER
                     GNLLFRSEAAAKHNPAGNKSLIYKSRESIIEQIEKAKKNNLYLIARVVTFKDDDYAKN
                     NPESAIFDNETKDTYIDRDKQRWVSPHNRKYWEYLIELCKEAADLGFNEIQFDYIRFT
                     DWKTNLDFRNTERESKSLAIQRFLKYAREELSKKEIYVSADIFGLVASASDDLNLGQY
                     WEGISNVVDFVSPMIYPSHFSYGFGGIPIPDTEPYKIVYISARDSVARNKNIETPAII
                     RPWIQDFTAYWLKGHINYEKQQLTDQIKALSEHGIDEYLLWNPKNQYKYLK"
     gene            complement(2172..2393)
                     /locus_tag="PL056_13400"
     CDS             complement(2172..2393)
                     /locus_tag="PL056_13400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48057.1"
                     /translation="MQAVVDQWKNAADELRKIKDDELSQLKYDWKIVDALLEIGARTG
                     KNSKTSGLVEMQRLFSVLLKKMETQTSHN"
     gene            complement(2411..>2598)
                     /locus_tag="PL056_13405"
     CDS             complement(2411..>2598)
                     /locus_tag="PL056_13405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48058.1"
                     /translation="DLFIMKAFASRPKDWLDAEGIAKKQKSSLDRIYIMKHLEILAEL
                     KEEPEIITKSEKILGLK"
BASE COUNT          816 a          525 c          486 g          771 t
ORIGIN      
        1 attgcagatc cgttgtttga aaatccggta actgttcctg tttcaaggtc ggttccgtta
       61 attgtgtagg ttattgatct gtctgtcatc cacgatattt cagaaacata tttttctttt
      121 atttgggttt cgttcatttc aaaaaaagtg tcaagtgttc tgataagttc agctaaaacc
      181 gcttctgttg taatatctgg gacatattcg tttaatgcac cgactttcca gttatgttca
      241 aacagggttg tttcaggcat gaaaacattg atccctattc cgcagacata gaatctgtca
      301 accccgcgct gaacggtttt tgcaattatt ccagcacatt tattcatgtc agccagaata
      361 tcgtttggcc attttattga tatatttcca gtgtctacaa actttttcac tgccatctgt
      421 gctgaaaggg cgatcctttg cggaatgaga ttcaacggct ctgatctatc cagtttctca
      481 aaaaaagaaa ggtacagatt cccgttatct ggggaaaacc acgatttgcc gttcctgccg
      541 taaccgccac tttgcaagtc agcgacaact attccgcgat tcaccccttt ttcaataaat
      601 tcaatggctt tatccatcgt tgaagaaacc tcgccaaaat ggtaaaaccc cgaaaacctg
      661 ttctttggaa taatgtattc tttcattttt cccccaaaaa atacaaggac aagctgaata
      721 tcatagaaaa aaccgggttt gtcaacttgt aaaaaaattc acaaaaatag tgctttaaaa
      781 tattgcttat gggaaaagga tttaatatta ttacacgatt ttatgagccg aggggaaatg
      841 caccgttttt ttatatttat ttcgattcta tttttttcaa tcaatgcata ttcagaaaac
      901 agcgaagtgt ttgtaaatgc tgatattttg aacataagaa cctatcccac cacaaattcc
      961 gctaaaactg tgctggcata cagaggtgac attctgatca tttttgagag taaaaaagat
     1021 tccgaaggga aagagtggat aaaagttgct ctaaaaaaga aatcatctga agccgtcggc
     1081 tgggttcttg ccgaatacac cgttaaaaca agagcaagtt tgttaaatca gatatacaaa
     1141 gatctcgatt attctcctca gaataaaact gatggataca aaaacaaccc taaagtgaaa
     1201 gttaaaggaa tatatctcac ccgctattct tcaactccta cccggcttaa ccacttcttt
     1261 aatcttgtga agggaacaga gataaacgct ttcgtaattg acatcaagaa cgaaaggggc
     1321 aacctgctat tcaggagtga agcggctgca aagcacaatc ctgccggcaa taaatcgctg
     1381 atctacaaaa gcagggaatc catcattgaa cagatagaaa aagcaaaaaa gaacaatctc
     1441 taccttatcg caagagttgt gacattcaaa gatgacgact atgcaaagaa taatcccgaa
     1501 tctgcaattt ttgataacga aacaaaggac acatatatcg acagagacaa acagaggtgg
     1561 gtttcgccgc acaaccgcaa gtactgggaa tatctcatag aactctgcaa agaagcggca
     1621 gatctcggat ttaatgagat ccagtttgac tatatcagat ttaccgactg gaagacaaac
     1681 ctcgatttca gaaatactga aagggagtca aaatcactcg caattcagcg gtttttgaaa
     1741 tatgcaagag aggaattgag caaaaaagag atctatgtga gtgccgacat tttcggactt
     1801 gtcgcttcgg caagcgatga tcttaatctc ggtcagtact gggaagggat aagcaatgtt
     1861 gtcgatttcg tttcaccgat gatctatccg tcccacttct cttacggttt cggtggaatt
     1921 cccatccctg acacagaacc gtataagatc gtttatattt cagcaagaga ttcagtcgca
     1981 cggaataaaa atatcgaaac acctgcgata atcaggccgt ggatacagga ttttacagct
     2041 tactggctta aaggccacat caattatgaa aaacagcagc ttacagacca gatcaaagca
     2101 ctaagcgaac atggaataga cgaatatctt ctgtggaatc ccaaaaatca gtataagtat
     2161 ttgaagtaag attagttatg gctggtttgg gtttccatct tttttaaaag aacggaaaag
     2221 agtcgctgca tttcaacaag tccgctcgtt tttgagtttt ttcctgttct tgcaccaatt
     2281 tcaagaagag catcgacgat tttccagtca tattttaatt gcgatagctc atcatccttg
     2341 attttgcgaa gttcatcagc agcgttcttc cactgatcta caacagcttg catctgctcc
     2401 tttgtcaaac tcattttaat ccaagtattt tttcagattt agtaattatt tccggctctt
     2461 cctttaattc cgccaatatt tcaagatgtt tcatgatata aattctgtca agcgaggatt
     2521 tttgcttttt tgcaatgcct tccgcatcca gccagtcttt ggggcgtgat gcaaaagctt
     2581 tcataataaa aagatctt
//
LOCUS       DAOOYB010000360         2595 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_49375_length_2595_cov_9.411811, whole genome shotgun sequence.
ACCESSION   DAOOYB010000360 DAOOYB010000000
VERSION     DAOOYB010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49375_length_2595_cov_9.411811"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="PL056_13410"
     CDS             <1..183
                     /locus_tag="PL056_13410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48059.1"
                     /translation="GIEKGIEKGIEKGIEKGAFAKAVETCRNALKEGAAPEFISKITG
                     LTIEKIKEIQKTLQTD"
     gene            312..1523
                     /locus_tag="PL056_13415"
     CDS             312..1523
                     /locus_tag="PL056_13415"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HPM48060.1"
                     /translation="MTSNFDTKNNNCYVSCDNTYQSCFDACNGDPTCEAGCLATKNTC
                     DSNCAATDYAGNVHNFLWGGSLYEPETNLYWMRNRYYHKDMHRFINQDPIGIWGDANN
                     LGNGFAYVAGMVIEASDPTGLGPLDQKDGESDEEYQNRVDGMRRNTAKPSFVLEFAAM
                     SVRESAIEDAKSFFGVDFIENLIEFQKNPNFNNGVKTWLALTSIGNLPKEAKDFAGKS
                     VKALGCAIVAMETIQDGVLYKLTDKNGNIYYMSGDGTIYRCVNGQWQKLVYVGWFKWE
                     WQDVSSPPPGYNKRPSDPDSIDGRQTYFMNQFLKKMLEVKDKKRFPEIMEEEGPFTEY
                     IDENGKKIFVRNPYYRGKDPMEEILWRVQEDSGMWGFVRNPFAPGADTSKQKELRKLI
                     ESDGVIGPSIF"
     gene            1702..2451
                     /locus_tag="PL056_13420"
     CDS             1702..2451
                     /locus_tag="PL056_13420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HPM48061.1"
                     /translation="MENGKIIDESHYLKWATLGCDNNDPQACGELSKFWDNGKEKAKD
                     EEKSFMYLKKGCELFSKTDFAQNDENSRRNKLAILDNCDMLSGIYYSNEKSSFFDIEK
                     AIELNILLCKEKESSYNCHFAYSRLKKLGKESESLKWLTFGCVEKHEFFSCLTFLDII
                     DSGKKEEKADLFYNMLERTKTDNDLWLYSFARAIWVIKKDSKETTKKMKEFLKIRKIQ
                     LKKMENDEFFKEYMETEEFKKLKEEFKDKFE"
BASE COUNT          912 a          364 c          575 g          744 t
ORIGIN      
        1 ggaattgaga aaggaattga gaaaggaatt gagaaaggaa ttgagaaagg agcttttgcc
       61 aaagctgttg agacttgcag gaatgcgttg aaagagggtg ctgcaccaga atttatttct
      121 aaaattacag gtctaaccat tgaaaaaatc aaagaaattc agaaaactct tcagacagac
      181 taagttttta ttatagaaca caaaatctat cagtaaaata taattgaaag agagaatctg
      241 ttgcattaac tgatgattct ggtaatattc ttgagcgtta caggtacaga gtctatggag
      301 aacatgagat attgacttca aactttgata caaaaaataa caactgctat gtttcctgcg
      361 acaatacata tcagagttgt tttgatgcat gtaacggaga tcccacctgt gaagccggct
      421 gtcttgccac aaagaacaca tgtgattcaa actgtgccgc aactgattac gccggaaatg
      481 tccacaactt cctctggggc ggctcactct atgaaccgga aaccaacctc tactggatga
      541 gaaacagata ttaccacaaa gatatgcatc gcttcatcaa tcaggacccc atcggcatct
      601 ggggagatgc aaacaacctc ggaaacggct tcgcctatgt agccggaatg gtgattgaag
      661 cgagtgatcc gacagggttg ggtccgttgg accaaaaaga cggagagtct gatgaagaat
      721 atcagaacag agtggatgga atgagaagaa atactgcaaa accatctttt gttcttgagt
      781 ttgcggcaat gtctgtaaga gaaagtgcta tagaggatgc aaagagtttt tttggagttg
      841 attttattga aaacctgatt gaatttcaaa aaaaccctaa ttttaacaac ggagtaaaaa
      901 catggttagc attaacttct attggaaatt tgccaaaaga agcgaaggat tttgccggca
      961 agagtgttaa agctttaggt tgtgcaattg ttgcaatgga aaccatccaa gatggcgttt
     1021 tgtataagtt aacagataaa aatggaaata tttactatat gtctggtgat ggaaccattt
     1081 acagatgtgt taatggtcaa tggcaaaaat tagtttatgt tggatggttt aaatgggaat
     1141 ggcaagatgt tagcagtcct cccccgggat acaataagcg tccatcagat ccggattcaa
     1201 ttgatggcag gcagacgtat tttatgaatc agttcttaaa aaaaatgctg gaagttaaag
     1261 acaagaaaag atttccggaa atcatggaag aggaaggtcc gttcacggaa tatattgatg
     1321 aaaatggaaa gaagattttt gtgcgtaacc catactacag aggcaaagat ccaatggaag
     1381 aaattttgtg gcgggtacaa gaagattcgg gcatgtgggg atttgtccga aatccctttg
     1441 cacccggagc tgacacatca aaacaaaagg aattgagaaa attgattgaa tcggatggtg
     1501 ttataggacc gtcaatattt taaaagaatt ggggagatat atatgaaaag aatcgtatta
     1561 atgagaatct ttatgatatt gtattttatt atatgttttt ttgctagctg taacgaagtt
     1621 gtgcccgcta atctgtcggt gtttgatatg tgtgaaaagg aaaaacgggg agatatttgt
     1681 tacagcatag cttatcttgc agtggaaaat ggaaaaatca tagatgaaag ccattattta
     1741 aaatgggcta ctttgggttg cgataataat gatcctcaag cttgtggcga attgtctaaa
     1801 ttttgggata acggaaaaga aaaagcaaaa gatgaagaga aatcgtttat gtatttaaaa
     1861 aaaggatgcg aattattcag caagacagat tttgcccaga atgacgaaaa cagcaggaga
     1921 aataaactgg caatactcga caattgtgac atgctttcgg gaatatatta tagcaatgag
     1981 aaaagctctt tttttgatat tgagaaagct attgaattaa acattcttct gtgtaaggaa
     2041 aaagaatctt cgtataattg tcattttgct tattccagat taaaaaaact gggaaaagaa
     2101 agtgaatccc tcaaatggtt gacattcggc tgcgttgaga agcatgagtt ttttagttgc
     2161 ttgacttttc tggatattat tgattccggg aaaaaagagg aaaaagcaga tttgttttat
     2221 aatatgctgg aaaggacaaa aacagacaat gatttgtggc tttattcctt tgcaagagcg
     2281 atttgggtga ttaaaaaaga ttccaaagaa acgacaaaaa agatgaagga gtttctgaaa
     2341 ataagaaaga ttcaattaaa gaaaatggaa aatgatgagt tttttaaaga atatatggaa
     2401 actgaagaat ttaaaaaact caaggaagaa tttaaagata aatttgaatg acaaaagcca
     2461 taacaatttt gtttttttcg agtattattt tttcaagaat tctgggggaa atagttctta
     2521 atagtcacca tgctttttta tactacattc ttccgcatca ctactgctgg ttttcgttcg
     2581 tttttttatg gtttg
//
LOCUS       DAOOYB010000361         2593 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_49429_length_2593_cov_6.328211, whole genome shotgun sequence.
ACCESSION   DAOOYB010000361 DAOOYB010000000
VERSION     DAOOYB010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49429_length_2593_cov_6.328211"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..2408)
                     /locus_tag="PL056_13425"
     CDS             complement(84..2408)
                     /locus_tag="PL056_13425"
                     /inference="COORDINATES: protein motif:HMM:NF019271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48062.1"
                     /translation="MRTVSLIFGFIVIFLINGCFCNEKSDKPEPVEEKEPSSCADIKC
                     KDNSSCENDIWGGGCSGNVSLSCRCDDGFESTETSFDKFGNTFEHCVDIDECADGNIC
                     GYGRRCTNTEGSYYCEEIDECAENQGVCGPLDCLNTEISYKCECPSGNIFEFNRFVSL
                     ETLIEAVTVDDMHYTLYLSNNEIYDYSFDSSDPGALLSKPVLSATAYFDKGTVLGMVS
                     YENNIFMICENGVYLIERESDGKLNIREEPLFLMNGTSAWAEDSGVLVMAGYDDESND
                     NALFFLDLTAGDIPSSLHTLQLNNADAVNVLGYRYNAAVLIGYLDDPGVSMNIKSMSG
                     TSPEEFSLKSEIQIEPLDDDGWGGDLYENMAIEITEDKAFVIRSGYMDILKLADDGTL
                     QHTYFMDSSILSDWIVEKDQLIQFRNYYEPPYKVFHWDITDPYDPQNFLTIDTDYSAA
                     GVLKNNDVFFIREFTKTNGTGTKIVTDLEDDPVHTVFTEYSFTGYSSHAVSYSSGLTE
                     IKLCSDFSETYYSDPVILENNGTLYIHNNTGIDIYGCDVEEFQVLEKNAFTVSERFAK
                     EKDRLLIKSPDGIVVIYNGTGFGYYRLSEDRTQVEGMLKDGIEIGEGTVRSESEVVSA
                     AILGNRLYLVDSNNVLTFGDFDNFDKTAVSIDIESNIFAPSEKGIYVKRGNAVYYYDT
                     VFNKEPLRLDTIVDLKRIFAVNGYLVTQTSSSITVWVRSTDGSPVQVEEKIILSQIPD
                     TANVYIAEAHVEGEAVFFSTIDGDIYKLSLPDCF"
BASE COUNT          794 a          551 c          424 g          824 t
ORIGIN      
        1 ataagatcaa atccgcaatt agtataatca gcaaaaaaaa attgtgaaac ttttatgaat
       61 taatgctgaa aaattatttc agattaaaaa cagtcaggaa gtgacagttt gtaaatgtct
      121 ccatcgatcg tgctgaaaaa aacagcttcg ccttctacat gagcttcagc aatataaaca
      181 ttagcagtat cgggtatttg actcagaatg attttttctt caacctgtac cggtgatccg
      241 tcagttgaac gcacccagac agttatcgat gatgaagttt gagttacaag ataaccgtta
      301 acggcaaata ttctcttcag atctacaatt gtatcaagcc gcaaaggttc tttgttaaaa
      361 acagtgtcat aatagtaaac agcatttcct ctttttacat agattccttt ttctgatggt
      421 gcaaaaatat tcgattctat gtcaattgaa acagcggttt tatcaaaatt atcaaaatct
      481 ccgaatgtta aaacattatt actgtcaaca agatacagtc tgtttccaag aattgcggca
      541 ctgactacct cagactctga tcttactgtt ccctcgccta tctcaatacc gtctttaagc
      601 attccttcaa cctgcgtgcg atcttcactc aggcgataat atccgaatcc tgtaccgtta
      661 tatattacaa caataccgtc cggtgattta attaacagcc ggtctttctc ctttgcaaat
      721 ctttctgaaa ctgtaaatgc attcttttca agcacttgaa attcttcgac atcgcaacca
      781 tagatatcaa taccggtatt attgtgaata tataaagtgc cgttattttc aagaattaca
      841 gggtcggaat aataggtttc tgaaaagtca gaacacaact ttatttcagt aagaccggaa
      901 gagtatgata cggcatgcga tgaatagccg gtgaaactgt actcggtaaa aacagtgtgg
      961 accggatcat cttcaagatc cgtaactatc tttgtgcctg ttccatttgt tttggtaaat
     1021 tctctgataa aaaagacatc gttgttcttc aatacccctg ctgcggaata atcggtgtct
     1081 attgtaagaa agttctgagg atcataagga tctgtaatgt cccagtgaaa aaccttatac
     1141 gggggctcat aataattacg aaactgtata agttgatctt tttcaacaat ccaatctgac
     1201 aatattgaag aatccataaa atatgtgtgc tgaagcgttc cgtcatcagc aagttttaat
     1261 atatccatat atccgctgcg aattacaaaa gctttgtctt cagtaatttc tattgccata
     1321 ttttcgtata aatctcctcc ccatccgtca tcatccaatg gttcgatctg aatctcactt
     1381 tttaacgaaa attcttcggg ggatgttccg ctcatacttt ttatgttcat ggacacaccg
     1441 ggatcatcaa gataacctat taaaacagcc gcattatatc tgtatccaag aacatttaca
     1501 gcgtcagcat tattcaattg cagggtgtgt aagcttgacg gaatatctcc ggctgtaaga
     1561 tcgagaaaga aaagggcgtt atcattgctt tcatcatcat atcccgccat aacaagaact
     1621 cccgaatctt ccgcccaggc actggtgccg ttcatcaaaa agagtggctc ttcccttata
     1681 ttcagttttc catcagattc ccgctcaatc aagtagaccc cgttttcaca gatcatgaat
     1741 atattattct cgtaagacac cattccaaga accgttcctt tatcaaaata cgctgttgcg
     1801 cttaataccg gcttgctcaa aagtgctccg ggatcactac tgtcaaatga atagtcatat
     1861 atttcattgt tggaaagata taaggtatag tgcatatcgt caacagtaac ggcttcgatt
     1921 aaagtttcca gtgacacgaa cctgttgaat tcgaatatgt ttccggaagg gcattcgcat
     1981 ttatatgata tttcagtgtt caaacaatcg agcggaccgc agacaccttg attttcggca
     2041 cattcatcga tctcctcgca ataatatgaa ccctcagtat tagtgcatct tcttccataa
     2101 ccgcatatat tgccatccgc acattcatca atgtcaacgc aatgctcaaa agtgttgccg
     2161 aacttgtcaa aagatgtttc cgttgattca aatccgtcat cacaccgaca cgacaaactg
     2221 acattcccgc tgcatcctcc tccccagata tcattttcac aagaactgtt gtctttgcat
     2281 ttaatatcag cacaggatga aggttccttt tcttcaacag gttcaggttt atcgcttttt
     2341 tcattgcaga aacatccatt tataagaaat attacgataa aaccgaaaat taatgaaaca
     2401 gttctcattt tacctccttt tttcaacagc atttctttag tcgttttttg tactgttttt
     2461 tatttaaaat atttatcaag cttcagatta cttcactttt tataatttgc cgttatcatt
     2521 aaaaagctca ttgaacttaa atggagatca ttatgaaaag tcaccgaaag gaactttggt
     2581 ttcacactgc aaa
//
LOCUS       DAOOYB010000362         2558 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_50413_length_2558_cov_9.131043, whole genome shotgun sequence.
ACCESSION   DAOOYB010000362 DAOOYB010000000
VERSION     DAOOYB010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50413_length_2558_cov_9.131043"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..958
                     /locus_tag="PL056_13430"
     CDS             <1..958
                     /locus_tag="PL056_13430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48063.1"
                     /translation="SLFQPFLKDTSFTGTVSGEGDICLSEGRKFNGKIDIKGTDVIFR
                     GKVPTPMGDFDVGKIDLGSVEFFAKVEDSKAEIEKFLINGIVALDVIGRIMLNAKSAV
                     SSRIDLDVRAEVPDMKRISENAALNLLVGQMSQYKGEKENSYAFMLRGFMTKPVMSKA
                     PEKRSVKGANPEKSETVSKRSDRKRKPLANRKRPEPVTPSLNRPKPGNDDLKEDPKED
                     PKEESKEEPKEDPKEESKEEPKEEPKEEPKEEPKEEPKEEPKEEPKEVRMEESRPVRA
                     VEETVKEAEKLPVQEEEDQNPSNTEEKRKKEDSHDGETNDED"
     gene            989..1510
                     /locus_tag="PL056_13435"
     CDS             989..1510
                     /locus_tag="PL056_13435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48064.1"
                     /translation="MKKLIWLAVLFSMFFSVSCNKKYIKGTEIEENEDSKTILKIFAH
                     YVKGFKDQNPDLFVPYISSKYYDNNGTDDASDDVDYDRVVEMLNSEQFKSLEKVEVVY
                     ILKDLKIDKKENKGKLLFYFEVRFKMTSKLPPEEGGDSFYKPDGKTNHKISEHNQMKF
                     VKEDGGWMIVSGL"
     gene            1619..>2558
                     /locus_tag="PL056_13440"
     CDS             1619..>2558
                     /locus_tag="PL056_13440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48065.1"
                     /translation="MFSYFKVLSFFKVFLFVAFMLISGGVSAQMGPEVSVDSNGKILK
                     WEEGSRDYFVMYKSVLLNLNQTKCLQDCQPGQDKAGNPQADACVDPNVGTTFTLMNNH
                     IPSDAHIEAAYLVWTTAVERDKFDAMVDNSVTLTFTGKDGNVTHSAEITGPEHYLDES
                     DGFTYEGKRLEFPLMTGYANPYKTCNVDADCHETIGTYSECVPYEGKTYCGLRQGSYT
                     YRIDVTDFFETIHELGRDAGISVDGFQLLGNYNVSGIPCTNSSHYLDISGMIGGWSII
                     MVYTSEKIFPKKIYIYDDLDIYQFQYTDINVSGFELP"
BASE COUNT          864 a          430 c          591 g          673 t
ORIGIN      
        1 ttctcttttt cagccatttc tcaaggatac ctcttttaca gggacagttt ccggtgaagg
       61 tgacatatgt ttgagcgaag gacggaaatt taacggaaag atagacataa agggaactga
      121 tgtcattttt agaggcaaag tgcctacacc gatgggtgat tttgatgtcg ggaagatcga
      181 tcttggcagt gttgaatttt ttgcaaaagt tgaagacagc aaagccgaaa tagaaaagtt
      241 tcttatcaac ggaatagtgg ctctggatgt gataggcaga ataatgctga atgcaaagag
      301 tgcagtatca tcacggatcg atcttgatgt aagggccgaa gttccggata tgaaaagaat
      361 atcggaaaat gctgcactta accttcttgt aggacaaatg tcgcaatata aaggtgaaaa
      421 agaaaattcc tatgcgttca tgttgagggg atttatgacg aaacctgtta tgtcaaaagc
      481 tccggaaaaa agatctgtaa aaggggcaaa tcctgaaaaa tctgaaaccg tatcaaaaag
      541 aagcgacagg aaaaggaaac ctttggctaa tagaaagagg ccggaacccg tcactccctc
      601 cctgaacagg cccaagccgg gtaatgatga cttaaaggaa gatccgaaag aggacccgaa
      661 agaagagtcg aaggaagagc cgaaagagga cccgaaagaa gagtcgaagg aagagccgaa
      721 agaagagccg aaagaagagc cgaaagaaga accgaaagaa gagccgaaag aggagccgaa
      781 agaagaaccg aaagaagtta gaatggaaga gagccggcct gtcagggctg ttgaggaaac
      841 tgtgaaagaa gctgaaaaac tccctgttca ggaagaagag gatcaaaacc catcaaatac
      901 tgaagaaaag agaaagaaag aagattctca tgacggagaa acaaatgatg aagattaaag
      961 ataaatagac aatttaggga gatatcaaat gaaaaaatta atctggcttg cagttttgtt
     1021 ctcaatgttc ttttcggttt cctgtaataa aaaatacata aaagggactg aaattgagga
     1081 aaacgaagat tccaagacga tcctgaagat ctttgcccat tatgtaaagg gtttcaaaga
     1141 tcagaaccct gatcttttcg tgccctatat ttcttctaaa tattatgaca ataacggtac
     1201 tgatgacgca agtgacgatg tggattatga tcgtgtcgta gagatgttga attccgaaca
     1261 gttcaagtca ctggaaaaag tagaagttgt gtacattctc aaagacctca agatagataa
     1321 aaaggaaaac aaaggaaaac tcctttttta ttttgaagtg agatttaaaa tgacaagcaa
     1381 actgcctcct gaagaaggtg gagattcttt ctataaacct gacggtaaga caaatcataa
     1441 gataagtgaa cacaatcaga tgaaattcgt caaagaagat ggcggctgga tgatagttag
     1501 cggtctttag aaacactctt tttaataaat atcttttaat aaatcgcttg acatgatata
     1561 ataaaaaatt atacttgaat ccggcggtat gacggtcatt ttcttcaaga ggtataaaat
     1621 gtttagttat tttaaagtgt tgtctttttt taaagttttt ctgtttgttg cttttatgct
     1681 catttccggg ggagtttccg cccagatggg gccggaagtg tctgtcgatt ctaatggaaa
     1741 aattctgaaa tgggaagaag ggagccgtga ttatttcgta atgtataaat cggtacttct
     1801 taatctcaac cagacaaaat gccttcaaga ttgtcaaccc gggcaggaca aagcaggaaa
     1861 tcctcaagcc gatgcatgcg tagatcctaa tgtcgggacc acatttactt tgatgaacaa
     1921 ccacattcct tctgatgccc atatcgaagc ggcatatctt gtctggacaa cggctgttga
     1981 aagggataag ttcgatgcaa tggtggataa ttcagttacg ctcactttca cgggcaaaga
     2041 tggaaatgtt actcattccg ctgagattac aggtccggag cattatcttg atgagtcgga
     2101 tggttttact tatgaaggga aacggcttga atttcccctt atgactggtt atgcaaaccc
     2161 ttataaaacc tgtaatgttg atgctgactg ccatgaaacg ataggaactt attcagaatg
     2221 cgttccttat gaaggaaaga cctattgcgg attgagacag ggttcataca cttaccgtat
     2281 tgatgtcacc gatttttttg aaacaattca cgaattaggg cgtgatgcag gtatttctgt
     2341 tgacggtttt cagcttctgg ggaattacaa tgtaagcgga attccctgca ccaactcatc
     2401 tcattatctt gacatttcgg gaatgatagg gggctggtca ataataatgg tttacacatc
     2461 tgaaaagata tttccaaaga agatctatat ttatgacgat ctcgacatat atcagttcca
     2521 gtataccgat ataaatgttt ccggctttga gcttccga
//
LOCUS       DAOOYB010000363         2541 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_50907_length_2541_cov_10.771923, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000363 DAOOYB010000000
VERSION     DAOOYB010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50907_length_2541_cov_10.771923"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="PL056_13445"
     CDS             <1..438
                     /locus_tag="PL056_13445"
                     /inference="COORDINATES: protein motif:HMM:NF016347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HPM48066.1"
                     /translation="ISDLTTSGVEKIEIIQTYFGDKGYWDNDIFTPSGLEECIVKGLE
                     QSMDTVPPEISIVKRFGPYSNDVITQYNQEYTACFLTFPNAEDHLRNIEKKNKNVIAI
                     GPERGFTKYENSQFIKAGFIPVKLGNRIIRTEAAVHAAVAGFI"
     gene            508..2478
                     /locus_tag="PL056_13450"
     CDS             508..2478
                     /locus_tag="PL056_13450"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HPM48067.1"
                     /translation="MKVMIVEKPSMKRKMEAALGNEVKVVTSVGHIESLMDLEFYLQD
                     HFPKDKKPFWSETLKYLPFIPEKFRHMITNKPVYAEIEKALKNATEIILGADPDREGE
                     LIHRNILEIAKENGAVKAEKITRIWLHSETAPEIKKAFQARQDHKEFDGYYNAARTRE
                     IVDWLVGIQLTILYSVKYGKPGKPVSIGRVQTWLLAEIIKRFIENRDFKPEPFRTFLF
                     LTKEGVVFNMIDEDERVFKTTDLKEADKLFNSLHGKDLLITGVKKKKFVEYAPSLYDL
                     KALQKDAAQRYNIAPDDTLKIAQSLYEKHDLISYPRTDCSVLSESEAKELKNAMNLVY
                     RFDEYKQLVMAVKKQNPELILNSKYIGKLEGHYAIIPVLSYDKSTVPKLSEQERLIFD
                     LIVKRFIATLLKPAKGETTEFTAKIDESLFNSKFKNYTEPGFLEFIKPDRKKEGEGEE
                     DKILSVSYKKNDSVKGDLEMKEDMTKPPKLYKDASLLTLMEKAHLQIKDPGLKKALKE
                     ADGIGTAATRASFPPLLIKRGYITKETDGTYIPTDLGMKIYEILPEELVIPDFSANLE
                     QELSGFIKKKPVKSVEEIIRETETFIRKVFKNFKEPPRVFNGKLSDKQIAIIEKHGDQ
                     KIKESLKTGDFQACHTWIDNFFKMLDERKKKK"
BASE COUNT          884 a          465 c          549 g          643 t
ORIGIN      
        1 atatcagacc tcacgacttc cggagttgaa aaaattgaga taattcagac atatttcggt
       61 gataaaggtt actgggataa cgatattttc accccttcag gtcttgagga atgcattgtc
      121 aaaggtcttg agcagagtat ggatacagta cctcctgaaa tatcaattgt caaaagattc
      181 ggcccttatt cgaatgatgt gatcactcaa tataatcagg aatatacagc atgtttcctc
      241 acttttccga atgcagagga tcatttgagg aatatcgaaa aaaagaacaa aaatgtaatt
      301 gcgataggcc cggaaagggg ttttacaaaa tatgagaaca gccagttcat aaaagcaggg
      361 ttcattcccg tcaagctcgg aaacagaata atccggacag aagcggccgt acacgccgca
      421 gttgcagggt ttatttaagt tatttgacaa acgccgcgca tctgatatta taagccaatt
      481 ttttctaaca atgattagga gaatagaatg aaggttatga tcgttgaaaa gccgtcaatg
      541 aagcggaaga tggaagcggc acttggaaat gaggtcaagg ttgtgacaag tgtcggtcac
      601 attgaatcgc tgatggacct tgaattttat ctgcaggacc attttccaaa ggataaaaag
      661 ccgttctgga gcgaaacgct gaaatatctg ccgttcattc ctgaaaagtt cagacacatg
      721 ataacaaata aacctgtcta tgcggaaatt gaaaaggcgt taaaaaatgc cactgagata
      781 attcttggtg ccgaccctga cagggaaggg gagcttattc ataggaatat ccttgaaatt
      841 gctaaagaaa acggtgcggt gaaagccgaa aagatcacaa ggatctggct tcacagcgaa
      901 actgcacccg aaataaaaaa ggctttccag gcaaggcagg atcataagga atttgacggc
      961 tactataatg cggcaagaac cagagaaata gttgactggc ttgtgggaat tcagttgacg
     1021 atcctctaca gtgtgaagta cggcaagccg ggtaaaccgg taagcatcgg tcgggttcag
     1081 acatggttac ttgctgaaat tattaaaaga ttcatcgaaa acagggattt taagccggag
     1141 ccgttccgga cttttttgtt ccttacaaaa gaaggtgtcg tttttaatat gatagatgaa
     1201 gatgaaaggg ttttcaaaac gacagatctt aaagaagcag ataaactttt caactcttta
     1261 catgggaagg atcttctgat aaccggagtc aaaaagaaga aatttgttga atatgcccct
     1321 tctttatatg atcttaaagc tcttcagaaa gatgcggcac agcgatataa cattgctcct
     1381 gacgacactt tgaagattgc tcagtcactt tatgaaaaac acgatcttat cagttatcca
     1441 agaaccgatt gttcagtttt aagcgaatct gaggcaaagg aactgaagaa cgcgatgaac
     1501 cttgtttaca gatttgatga atataaacag ttggttatgg ctgtaaaaaa gcagaacccc
     1561 gaacttattc tcaattcaaa atatattgga aagcttgaag ggcactatgc aataattccg
     1621 gttttaagtt atgataaaag cactgttccg aagctttctg agcaggaacg gctgatattt
     1681 gaccttatcg tgaaaaggtt tattgcaact cttcttaagc cggcaaaagg tgaaactact
     1741 gaatttactg caaaaattga tgaatctctt tttaattcca agtttaagaa ctatactgaa
     1801 ccgggatttc ttgaattcat taaaccggat cggaaaaaag agggtgaagg tgaagaagat
     1861 aaaatattat cagtaagtta caagaaaaat gattcggtaa aaggtgatct cgaaatgaaa
     1921 gaggacatga caaaaccgcc gaaactttat aaagatgcgt cactccttac tctgatggaa
     1981 aaagcccatc tccagataaa agatccggga ctcaagaaag cgctgaaaga agccgacgga
     2041 atcggaactg ccgcaacaag ggcatctttc cctccgttat tgatcaaacg gggctatatc
     2101 acaaaggaaa ctgacggaac atacattccg acagaccttg gaatgaagat atatgagata
     2161 cttcccgaag aactcgtgat ccctgacttt tcagcgaacc ttgaacagga actcagcgga
     2221 tttattaaga aaaaaccagt caaatcagtt gaggaaatca tcagggaaac tgaaacattc
     2281 atccggaaag tgttcaagaa tttcaaggaa ccgccaaggg tttttaacgg aaaactctcc
     2341 gacaaacaga ttgcaataat cgaaaaacac ggagatcaaa aaataaaaga atccctcaaa
     2401 accggcgact ttcaggcatg ccacacctgg atcgacaact tcttcaaaat gctcgatgaa
     2461 aggaagaaga agaaatgaga ttcctgatta tttttctgct tgacataata tttaacttca
     2521 ttagtatata cattgtagag c
//
LOCUS       DAOOYB010000364         2519 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_51578_length_2519_cov_10.250406, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000364 DAOOYB010000000
VERSION     DAOOYB010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51578_length_2519_cov_10.250406"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1382)
                     /gene="radA"
                     /locus_tag="PL056_13455"
     CDS             complement(<1..1382)
                     /gene="radA"
                     /locus_tag="PL056_13455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HPM48068.1"
                     /translation="MVKKKSSFICTNCSYSTPKWLGRCPECGEWNTLEEKDETVIPKD
                     NHRLFLSSPDAVFSHINNVTTTDSERINSGYKELDRILGGGIVKGSYNLISGHPGVGK
                     STLMLQAALEISRLCRVLYLSAEESVSQVKIRFERLAGKRGCGELYLSNENNLDKLRA
                     KVEDESFDFLFCDSIQSVYSPSVPGIPGSVSQIKECAVKLLETAKRKNITVFVIGHVT
                     KSGAIAGPMLLEHMVDSVLLFEGDPSLGYRVLRCSKNRFGPTDEIGIFTMTSTGLSEV
                     ENPSQFFLSDGMGSISGSALALILEGSRPFLIEVQSLATSSNLPQPRRVVTGIDAGRL
                     AMITAVLEKRCDIYFSNFDLFVNVVGGLKLKTPAVDLPVAASLLSSIFNIALPMNTAF
                     VGELGLGGEIREVPGLEIMLKEGSRLGLKRIYAPESRTKIKKSEGTEIIRIKSVYNII
                     EHIRSMNIFDS"
     gene            complement(1392..2441)
                     /locus_tag="PL056_13460"
     CDS             complement(1392..2441)
                     /locus_tag="PL056_13460"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HPM48069.1"
                     /translation="MNEKIYRLPNDERDYSFEEIAALILDGRLVKRSHIWEKSMTDWV
                     RLGACQDFTEIFKEYDLMAEEKVKQVLGTDDETQKKRELKQMMEDVGSEEQNIEVKKD
                     NHFKWVIIALLIIAAPVVFFSGYQLFIQKNIEDGPQEVKFDDINIEDIRFGSGVMKID
                     DIKGITVKKVAKKEEDLILKELLLEIRKENEAIESQEQQAESEKPKEKKKAGLFDTVT
                     DEELDAFRNSFMKKVGSKTVGSKVKTESSFASGGEELTQKQINEVVKNNYSSIKYCYD
                     KALKSNEGIGGKMEITLHIIGSGQVAKVINETPKFKGTEMDRCITEQIKKKWKFPPFN
                     GTLSTVTIPFLLSAQ"
BASE COUNT          637 a          582 c          475 g          825 t
ORIGIN      
        1 gagtcaaaaa tgttcattga ccggatatgt tcgatgatgt tgtaaaccga ttttatcctg
       61 attatttccg ttccttcgct tttcttaatt tttgttcggc tttcaggggc atatatcctt
      121 tttaatccaa gccgggaacc ctctttgagc ataatttcaa gtccgggaac ctctcttatc
      181 tctccaccga gtccgagttc acctacaaaa gctgtgttca tcgggagagc tatattaaaa
      241 atagaactta acaatgaagc ggcaacagga agatcgacag caggtgtttt cagcttcaat
      301 ccacctacga cattaacaaa aaggtcaaaa ttggaaaaat agatgtcgca tcttttttca
      361 agcactgccg ttatcattgc aagtcgccct gcatcgatac ccgtcacaac tcttctgggt
      421 tgaggcagat ttgagcttgt tgcaagtgat tgaacttcga tgagaaaagg tcttgagccc
      481 tcaagtatca aagcaagtgc cgaccccgat attgagccca ttccgtctga aaggaagaac
      541 tggcttggat tttcaacttc gctaagtccc gtcgaagtca ttgtgaaaat acctatttca
      601 tcagttggtc cgaaacggtt ctttgaacac cgcagaactc tgtatccaag agaaggatca
      661 ccttcaaaaa gaaggactga atcgaccatg tgttcaagga gcatcggtcc tgcaattgct
      721 ccgctcttcg taacatgacc tatgacaaaa actgttatat tttttctttt ggcggtttca
      781 agaagtttta ccgcacactc ctttatctga ctgacagatc ccggaattcc ggggacagag
      841 ggcgaataga cagattggat cgagtcacag aaaagaaaat cgaagctctc atcttccact
      901 tttgctctga gcttatcaag gttgttctcg tttgaaagat acagttctcc gcaccccctt
      961 ttccctgcaa gccgctcaaa tcttattttg acttgagaga ccgattcttc cgctgaaaga
     1021 taaagcaccc tgcacaacct tgagatctcg agagccgcct gaagcatcaa agttgactta
     1081 cctactcccg gatgtcctga aataaggttg tagcttcctt ttacaatccc tccgccgaga
     1141 attctgtcga gctctttata accggaattt atcctttcgc tgtcagttgt ggtcacattg
     1201 tttatatgac tgaaaaccgc atccggtgaa gaaagaaaaa gacggtgatt atctttggga
     1261 attactgttt catccttttc ttcaagagta ttccactcgc cgcattcagg acatcttcca
     1321 agccattttg gagtcgaata ggaacagttg gtgcaaatga aagatgattt tttcttgacc
     1381 atcttgactg ctcactgagc tgaaagaagg aacggtatgg taactgttga aagggttccg
     1441 ttgaatgggg gaaatttcca ttttttcttg atctgctctg ttatgcatct gtccatttcc
     1501 gtccctttga acttcggagt ttcgtttatg acctttgcca cctgaccgct tccgatgata
     1561 tgaagagtga tctccatttt tccaccgatc ccttcattgc ttttaagcgc tttgtcatag
     1621 cagtatttta ttgaagagta gttgtttttg acaacttcat ttatctgttt ctgagtcagc
     1681 tcttctcctc cggaagcgaa actgctttcg gttttgactt ttgatccgac agtttttgac
     1741 cctacttttt tcatgaatga atttctgaag gcatcaagtt cttcatcagt gacagtatca
     1801 aaaagtcccg ctttcttttt ttctttcggt ttttctgatt cggcttgctg ctcctgactt
     1861 tcaatagctt cgttctcttt ccttatttca agaagaagct ccttaagaat gagatcttct
     1921 tccttttttg ccactttttt aactgttatt cccttgatgt catcgatctt cattacgccg
     1981 gagccgaatc tgatgtcttc gatgtttata tcatcgaact taacttcctg cggaccatct
     2041 tcaatgtttt tctgtataaa aagctgataa ccactgaaaa aaaccactgg agccgcaatt
     2101 atcaataacg caatgatcac ccatttgaag tggttatctt ttttaacttc gatgttctgc
     2161 tcttcgcttc ccacatcctc catcatctgc tttagttcgc gttttttctg agtttcatcg
     2221 tccgtaccga gaacctgctt gaccttttct tctgccataa ggtcgtattc cttaaatatt
     2281 tctgtaaaat cctgacaggc accgagacgc acccagtctg tcattgattt ttcccagata
     2341 tgcgaccttt ttaccagtct gccgtcaaga ataagagctg cgatttcttc aaaactgtaa
     2401 tctctttcgt catttggaag ccggtagatc ttttcattca tgtgaaactc ctgaacaagt
     2461 ggaagaacac gcgcaaaata atactgtaca gcccgatttt tgtcaattta tagttcttt
//
LOCUS       DAOOYB010000365         2502 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_52093_length_2502_cov_8.979567, whole genome shotgun sequence.
ACCESSION   DAOOYB010000365 DAOOYB010000000
VERSION     DAOOYB010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52093_length_2502_cov_8.979567"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="PL056_13465"
     CDS             complement(<1..238)
                     /locus_tag="PL056_13465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48070.1"
                     /translation="MNEVLKKMKADIIEHISNYFDMCPERYCLKRSIEKEHLKRLLEF
                     ISRGKLIRGILVISSYDALGGKERKKILSIASAIE"
     gene            complement(231..2447)
                     /locus_tag="PL056_13470"
     CDS             complement(231..2447)
                     /locus_tag="PL056_13470"
                     /inference="COORDINATES: protein motif:HMM:NF012544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48071.1"
                     /translation="MKNVETKKLGTFLGVFTPTILTILGVIMYMRAGWVAGHMGLVNT
                     LLIVLIANSITLITTLSFSSVATNKKVGAGGAYYIISRTLGFAIGGAIGVPLFLSQVF
                     SITLYSYGLAEVLNFLIPWIPVAPVAFVIIIIVAALSFKGADFALKTQIPIMAAVAIS
                     LLFLAIGAFLKTDISSVRMSSPTGEVTFFTAFAIFFPAVTGIMAGLGLSGDLKDPQRS
                     IPLGAITATLVGFAVYLTVPFLLVMGADDKDLINDVMVWSEIAPAGALFIIPGLFGAI
                     FSSAVGSMLGAPRTLGALISDQFEKKSIIDFFNGSAGQKTVFGISLFIALSAVFLGNL
                     NDVATVVTLFFLTVYGVVNITAALEGLSNEPSWRPRFRIPWIISLAGGIACIVVMFLI
                     NPLASFAAIVIEFLLYVFLKRRSEKNIKGDARRGVYEKVIKNSLLNLRNHPMTARNWR
                     PYLQIVINDPQKQKNLVKFGSFFAENSGIVTVTQLIEGKLGEDLIDIDGKTQFMENFY
                     GQKDLTVFPEVNIVDDFEQGIITSVQANGIAGLQSNTVMICWPDEIKYLPKYLSIMRR
                     ISVIKKSFIIGKVSFDLKLLQKSSKPRSIHVWWGGLQRNGDMMLLLAYLLTTSVHWKN
                     SVISIINIVDSGEKELDAFFQLSHLASESRIAADIRIIVKDKDQSYVDIIHQTSKDAD
                     VVFMGLNTPEKGEEDQYALKLQELAGTLNAVFFIKNASVFKGTLLESDVYEGKYNE"
BASE COUNT          740 a          559 c          472 g          731 t
ORIGIN      
        1 gttctatcgc agaagcgatt gaaagaattt tcttcctttc ctttccaccg agagcatcat
       61 acgaagagat taccagtatt ccccgtatca gctttcccct tgaaatgaat tccagcagtc
      121 ttttaaggtg ttccttctca attgaccttt tcagacaata tctttcagga cacatatcaa
      181 aataatttga aatgtgctca atgatatcag ctttcatttt ctttaaaact tcattcatta
      241 tatttccctt catacacatc tgattcaaga agtgtccctt taaaaacact cgcgttttta
      301 ataaagaaaa cagcgttcag tgtccctgca agctcctgta actttaatgc atattgatct
      361 tcctcacctt tttccggagt gttaagcccc atgaaaacga catctgcatc cttactcgtc
      421 tgatggatta tatcaacata agattggtcc ttgtctttga caattatcct gatatcagcg
      481 gctatcctcg attcacttgc aagatgactt aactgaaaaa atgcatccag ttctttttct
      541 cctgaatcaa cgatgtttat tatgcttatg acagaatttt tccagtgaac gctcgttgta
      601 agaagatatg ccagcaggag catcatatca ccgttacgct gaagacctcc ccaccagaca
      661 tggatcgagc ggggctttga gcttttttga agaagcttca gatcaaaaga gacttttccg
      721 atgataaatg acttttttat tacagatatc cgtctcatta ttgaaagata tttgggcaga
      781 tatttaattt catcaggcca gcatatcata actgtgtttg actgcaaacc tgcaattccg
      841 tttgcctgta ctgaagttat tatcccctgc tcaaaatcgt caacaatatt aacttccgga
      901 aaaacagtca ggtctttttg accgtaaaaa ttctccatga actgagtttt gccgtcaata
      961 tcaattaaat cctctccgag tttaccctct ataagctgcg taactgtgac aattccggag
     1021 ttctctgcaa agaaggatcc gaattttaca agatttttct gcttctgcgg atcattgatc
     1081 actatctgca aatagggtcg ccagtttctt gcggtcatcg gatgatttct caggttgagc
     1141 agggagtttt tgatgacttt ttcataaaca ccccttcttg catcaccttt tatgttcttt
     1201 tcagatcgcc gctttagaaa aacataaagt agaaactcaa tgacaatggc cgcaaagctt
     1261 gcaagggggt tgatcaggaa cataacaact atgcaagcta tacctcctgc aagacttatt
     1321 atccagggaa ttctgaatct gggtctccag gaaggttcat tactcagacc ttcaagtgca
     1381 gcagtgatat taacgacccc gtacacagta aggaagaaaa gcgtcacaac tgtagcgaca
     1441 tcgttaagat tgcctaaaaa aacggctgag agtgctatga aaagtgaaat tccgaaaact
     1501 gttttctgtc cggcagatcc attaaaaaaa tcaatgatac tctttttctc aaactgatct
     1561 gatatcagtg cgcctaaagt tctcggtgct ccgagcatac ttcccacggc acttgaaaat
     1621 atcgctccga aaagaccggg aataatgaac aatgctccag ccggagcgat ttcggaccag
     1681 accattacat catttatcag atctttgtca tctgccccca ttacaagaag aaatggaact
     1741 gtaagataca ccgcaaaacc caccaatgtt gcagttatcg ctccaagcgg tattgatctc
     1801 tgcggatcct tcagatcgcc cgagagtcca agtcccgcca ttattccggt tactgccgga
     1861 aaaaagatgg caaatgctgt aaaaaaagtg acttcacctg tagggctgga cattcttaca
     1921 cttgaaatat ctgtctttaa aaaagctccg atagcaagaa aaagcagcga gatcgcaaca
     1981 gccgccatga tcggtatctg ggtttttaaa gcaaaatcag cccctttaaa tgaaagtgcc
     2041 gcgacaatta ttattatgac aaaagcaaca ggtgcaacag ggatccatgg aataagaaag
     2101 ttaagaactt cagctagacc gtaactgtat agggttatag agaacacctg tgacaggaag
     2161 agcggcacgc ctattgctcc gcctattgca aaacccagcg ttcttgagat tatgtagtat
     2221 gcaccgcctg cacccacttt tttattcgtt gcgacagatg aaaatgaaag tgtcgttatc
     2281 aaagttattg aattcgctat taaaactatc aggagcgtgt tgaccagtcc catgtggcct
     2341 gcaacccagc cggcacgcat gtacattatc acgccgagta tcgtcagtat agtgggtgtg
     2401 aaaacgccca ggaatgtacc gagttttttt gtttctacat ttttcatgag tcaaaaatgt
     2461 tcattgaccg gatatgttcg atgatgttgt aaaccgattt ta
//
LOCUS       DAOOYB010000366         2498 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_52217_length_2498_cov_14.448219, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000366 DAOOYB010000000
VERSION     DAOOYB010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52217_length_2498_cov_14.448219"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..850)
                     /locus_tag="PL056_13475"
     CDS             complement(<1..850)
                     /locus_tag="PL056_13475"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPM48072.1"
                     /translation="MESNRPLRVAIYTFGCKVNLFESDQLKNSLKNVEIVSADDQPDV
                     LIINGCTVTSATDSQVRNMIRRFSKLGRVIVSGCYARKNDEKLESFQNVEFVSDISEV
                     AEMLGGEFVYDANFNRSRPFVKIQSGCDQFCSYCIIPYVRGPLKSVPLNEIKTLVEKI
                     KTEQFHEVVLTGIHIGKYGKDLDDGSTLADVVKAAYEIIGRVRLGSLESPEIDEELFN
                     MAEKGMILPHWHIPLQHGCDSVLQRMNRPYSADDFKRAVDRLINIYKKLPAIGTDVIT
                     GFPGETD"
     gene            complement(837..1217)
                     /gene="nifU"
                     /locus_tag="PL056_13480"
     CDS             complement(837..1217)
                     /gene="nifU"
                     /locus_tag="PL056_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014183466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HPM48073.1"
                     /translation="MNYTEKVMDHFLNPRNIGKIEDADAVAEIGNATCGDIIKLYLKI
                     TSDGVIENITFQTFGCGAAIATSSMTTEMVKGKTIDEALEITREQVADELGGLPANKM
                     HCSNLAADALHKAIKEYKAKNGIK"
     gene            complement(1214..2416)
                     /locus_tag="PL056_13485"
     CDS             complement(1214..2416)
                     /locus_tag="PL056_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HPM48074.1"
                     /translation="MEHRIYLDHSATTPVRREVVDEIMPFLTEQGGNPSSRHKEGRIA
                     KTAFEKAREKVAAAINAEPKEITFNSCATEGNNTVLKGYMSGLRGNIRKNHIIITSVE
                     HPSIFNTAKSLEKDGFEVTFLPVDTKGALDLEILKNSIKENTGLISVMMVNNETGNIY
                     PVEEIARIAKEKNILFHVDAVQAIGKIPVDVKKIGCDFLTISAHKFYGLKGTGALYTK
                     WGKWVAPMISGGHQEKGRRPGTENVVGVIGMGRAIELAVSELDEESRRLKALRDHLEK
                     RILNEIPYTRIVGDPENRVPTITNISFRFIEGESIQLKLDNDGIAVSTGSACSSGTLE
                     PSHVIAAMKVDPEEAHSSIRFSLGKSSNLKDIEYTVERVKKIVEQLRSFSPVYDKFIS
                     ENETGGNR"
BASE COUNT          633 a          581 c          488 g          796 t
ORIGIN      
        1 catctgtttc accgggaaag ccggttatca catcggttcc tatcgcaggt aactttttgt
       61 aaatattaat taatctgtca actgcccttt taaaatcatc ggctgaataa ggtctgttca
      121 ttctttgcag aactgagtca catccatgct gaagaggaat atgccagtgg ggaagtatca
      181 tccctttttc agccatgtta aaaagttctt catcaatttc aggagactca agggaaccga
      241 gccggaccct gccgataatt tcataggccg ctttcacaac atcggcgaga gtcgagccat
      301 cgtcaagatc ttttccatat ttcccgatgt gaatgcctgt cagaacaact tcatgaaatt
      361 gctctgtttt tattttttca acgagtgttt ttatctcatt taacggaaca ctcttcaaag
      421 gaccgcggac ataaggaatt atgcagtatg aacagaactg gtcacaaccg ctctgaattt
      481 ttacaaaagg tctggaacgg ttaaaattcg catcatagac aaattcgcca ccgagcattt
      541 cagccacttc agaaatatct gaaacaaatt caacattctg aaaagattcg agtttttcat
      601 cattttttct tgcataacag ccgctgacaa taactctgcc gagttttgag aaccgcctta
      661 tcatgttcct cacctgactg tcggtggcgg aagttacagt acagccgttg atgataagca
      721 catcgggctg atcgtcagca gaaacgatct caacattttt tagagagttt ttaagctgat
      781 ctgattcaaa aagattgacc ttgcagccga aagtgtagat agcgacccgc agaggtctat
      841 ttgattccat tttttgcttt gtattctttg atcgccttgt ggagagcatc tgccgcaaga
      901 tttgagcagt gcattttgtt tgccggcagt ccgccgagtt catctgcgac ctgttccctt
      961 gtaatttcaa gggcttcgtc aatagttttg cctttgacca tttcagttgt cattgaagaa
     1021 gtggcaatcg ccgctccgca accgaaagtc tggaaagtta tgttctcgat aaccccgtcg
     1081 gatgtgatct tgagataaag cttgataata tctccgcaag ttgcgttccc gatctcggca
     1141 actgcatctg catcttcaat ttttccgata tttcttggat tcaggaagtg gtccatcact
     1201 ttttcagtat aattcatcgg tttcctccgg tttcattttc tgaaataaac ttgtcataga
     1261 ccggagaaaa gctcctaagc tgttcaacga tcttttttac ccgctcgaca gtatattcaa
     1321 tatctttgag atttgaagat tttccaagtg aaaaacggat actcgaatgt gcctcttcag
     1381 gatcaacttt catcgcagcg ataacatggc tgggctcaag cgttcctgaa gaacatgcgg
     1441 aacctgtcga aaccgctatc ccgtcattat cgagcttgag ctgtatcgat tcaccttcaa
     1501 tgaacctgaa tgaaatgttg gtgatcgtcg gaactctgtt ttccggatct ccgacaattc
     1561 tcgtgtaagg gatctcgttg agtatccttt tttcaagatg gtctctaagc gctttgagcc
     1621 gtctgctttc ttcatcaagt tcgcttactg caagttcgat cgctctgccc attcctatca
     1681 caccgacaac attttcagtt cccggtcttc tccctttttc ctgatgaccg cctgagatca
     1741 tcggagcaac ccatttgccc cattttgtgt aaagggcgcc tgttcccttc agcccgtaga
     1801 acttgtgagc tgaaattgta agaaaatcgc aaccgatctt tttaacatca accggaattt
     1861 tacctattgc ctgaaccgca tcgacatgga aaagaatgtt tttctccttt gcaatacggg
     1921 ctatctcttc aacagggtaa atgttccctg tttcgttatt aaccatcatt acgcttatca
     1981 gaccggtgtt ctcttttatc gaatttttca gtatctcaag atcaagagca ccttttgtat
     2041 caaccggcag aaatgtcact tcaaaaccgt ctttctcaag tgattttgct gtgttaaata
     2101 tacttggatg ttcaactgaa gtgattatta tgtgattttt cctgatgttt ccgcgaagtc
     2161 ccgacatgta acctttaaga accgtgttgt tcccttcagt ggcacaggaa ttgaaagtta
     2221 tctccttcgg ctcggcgttt attgcggccg caaccttttc ccgtgctttt tcaaaagcgg
     2281 tctttgcgat ccgtccttcc ttatgtctgc ttgaaggatt tcccccctgc tcggtgagaa
     2341 acggcattat ctcatcaact acttctcttc tgacaggtgt tgtagcactg tgatcaagat
     2401 atattctgtg ttccataagc cctcgtaaaa aaatcaaaaa acctgaaaca tactccagcg
     2461 gttaaatata gtttcgtatt gtgttttgtc aatcagaa
//
LOCUS       DAOOYB010000367         2489 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_52488_length_2489_cov_9.751027, whole genome shotgun sequence.
ACCESSION   DAOOYB010000367 DAOOYB010000000
VERSION     DAOOYB010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52488_length_2489_cov_9.751027"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            174..1295
                     /locus_tag="PL056_13490"
     CDS             174..1295
                     /locus_tag="PL056_13490"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HPM48075.1"
                     /translation="MISLVFLFSPIFSAEKDRLAVMDVADEAKLFNKTTIANVTDYIF
                     TKLQGTKIYWMVPKADRDTALEQAIEETVKGSRRECVDEKCQLSLVAQLQANFLINTK
                     IKKLYQGTCNISISKFDVEKRAGVDSWTTDFNCSEKGVFEAINSFDFGGKKEQVGIVK
                     QDDTADKNACKYAKEKNNLEIWEKYIEKYPKGECEFEATVRIEELNKKKSEKDENTKT
                     SSGKIIPPPKEKELKKPADLKVKNLSNWSKKAPKTMSWNDAKKYCENLVENGYSDWRL
                     PTISELRTLIKNCHATETGGACGVTDSCLSWKNCRNSACDGCSLDKSGKYSVWGDIGW
                     FWSSSVLSDRADGACDVYFHNGNVYSNYRMGSSNVRCVR"
     gene            complement(1308..1439)
                     /locus_tag="PL056_13495"
     CDS             complement(1308..1439)
                     /locus_tag="PL056_13495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48076.1"
                     /translation="MSENRKKIESQNEMTLEEMVSLISSENLHAEVSTVVAKGNEVW"
     gene            complement(1859..2191)
                     /gene="mazF"
                     /locus_tag="PL056_13500"
     CDS             complement(1859..2191)
                     /gene="mazF"
                     /locus_tag="PL056_13500"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018467586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HPM48077.1"
                     /translation="MVKYIPEQGDIVWLEFDPQAGHEQSGKRPAIVISHRIYNEKTGL
                     ALFCPVTTSIKGYPFEVKLPSIMKTTGVVLSDQIKSLDWIIRKAKFIEKVPRATLTEV
                     ITKFSKLY"
     gene            complement(2185..2418)
                     /locus_tag="PL056_13505"
     CDS             complement(2185..2418)
                     /locus_tag="PL056_13505"
                     /inference="COORDINATES: protein motif:HMM:NF015947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HPM48078.1"
                     /translation="MIKIMRWGNSLALRLPKAFLESLNLMEGSFVKIRTEKNRIIIEP
                     QNEMTLEEMVLQISSENLHAEVSTGVAKGNEAW"
BASE COUNT          768 a          465 c          485 g          771 t
ORIGIN      
        1 cgctcctgaa tttctttatg agatcagtgt tcccgctgat gaagccgctt aaactgaatt
       61 tttcagtttt cctctaaatt tgcaaggtca aaaagtttaa tgtataatcg gttttaagat
      121 ttttaacagt cgtttttatt gcgaggttga tgatgataaa gcggattttg ctaatgattt
      181 cattggtttt tttgttttct ccgatttttt ctgcggaaaa agaccgtctt gcagtaatgg
      241 atgttgctga tgaagctaag ctgtttaaca agacaacaat tgccaatgtt acagattata
      301 ttttcacgaa gttgcaggga acaaaaatct actggatggt tcccaaggcg gatagagaca
      361 cagctctcga acaggcgatt gaagaaaccg ttaaaggatc aagaagagaa tgcgttgatg
      421 aaaaatgcca gttgtcactc gtggcacagc ttcaggcaaa tttcctaata aatacaaaga
      481 ttaagaagct ttatcaagga acttgcaaca tcagtataag caaatttgat gttgaaaaaa
      541 gagctggagt agattcatgg acaactgatt ttaactgttc agaaaaaggt gtttttgaag
      601 cgattaacag ttttgatttt ggtggaaaga aggaacaagt gggcattgta aaacaagatg
      661 acacagctga caaaaatgct tgtaaatatg cgaaagagaa aaataattta gaaatatggg
      721 aaaaatatat tgaaaaatac ccgaaaggcg aatgtgaatt tgaagcaacg gtcagaatag
      781 aagaactgaa caaaaagaaa tctgaaaaag atgaaaacac aaaaacatct tcaggcaaaa
      841 tcattccgcc tccgaaagaa aaggaactga aaaaaccagc tgatctgaag gtgaaaaatt
      901 tgtccaactg gtcaaaaaaa gcaccaaaaa caatgagttg gaatgatgcg aagaagtatt
      961 gtgagaattt ggtggaaaat ggatattcag attggagatt gcccactata tcagaactta
     1021 gaacacttat aaaaaactgc catgcaactg aaacaggtgg agcttgtgga gttacagaca
     1081 gttgcctgtc atggaaaaat tgcaggaata gtgcttgtga cggctgttct cttgacaaaa
     1141 gtggaaaata tagtgtttgg ggtgatatag gttggttttg gtcctcgtcc gtgctctcag
     1201 atcgtgcgga tggggcatgt gatgtgtatt tccacaacgg caatgtgtat agcaactacc
     1261 ggatgggcag cagtaatgtt cgctgcgtcc gataaattca caaatcatca ccaaacttca
     1321 ttcccttttg caacaacagt agaaacctcg gcatgaagat tttctgaaga aatcagggaa
     1381 accatttcct ccagagtcat ttcattttgc gactcaattt tctttctgtt ttcgctcact
     1441 tcttcctctt cttctcatcg agcattttga agaagttgtc tgcctttttc cgtaatgcgg
     1501 tatttttgct tactgcttgt aggtttatcc ggaattgtca tttcaatcca accttcatca
     1561 attaatggct ttatgacttg gtccctgaac ttggtcctat tagttctatc aaaaatctcc
     1621 ataagctcaa gaattgtcac ttcattcaag cagttactaa gaattttaac ttgatcccga
     1681 cttagtcccg actcaatccc tgactcggcc cttaaaccgg atctgtaaaa tgttgctaag
     1741 cccggcaatc atcttgaatt catcaaaaga taaaatattt ttaagacctt gtatcattct
     1801 tttcctattt cttttttttc ttctcatcga gcatttttaa gaagttgttg atccaggttt
     1861 aatacagttt ggaaaatttg gtgattactt ctgtgagagt tgctcttggc actttttcga
     1921 tgaattttgc tttccttatg atccaatcaa gactttttat ctggtcggat aaaacaaccc
     1981 ctgtcgtttt cattatagat gggagcttta cctcaaacgg atatccttta atgcttgtgg
     2041 tgacaggaca gaaaagtgca agccccgttt tttcattata aattctgtgt gaaatcacta
     2101 ttgcaggtct tttccctgac tgttcatgtc ctgcctgagg atcaaattca agccagacaa
     2161 tgtcgccctg ctccggaatg tatttcacca tgcctcattc ccctttgcaa cacccgtaga
     2221 aacctcggca tgaagatttt ctgaagagat ctgcaaaacc atctcctcca gagtcatttc
     2281 attttgcggc tcaattatga ttctgttttt ttcagtcctg atcttgacaa aactaccttc
     2341 catcaaattc agtgactcaa gaaaagcttt cggaagccgc agtgcgagac tgttgcccca
     2401 acgcataatt tttatcatat tgcacctcct taatgctcta caatgtatat actaatgaag
     2461 ttaaatatta tgtcaagcag aaaaataat
//
LOCUS       DAOOYB010000368         2482 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_52707_length_2482_cov_9.815822, whole genome shotgun sequence.
ACCESSION   DAOOYB010000368 DAOOYB010000000
VERSION     DAOOYB010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52707_length_2482_cov_9.815822"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..253)
                     /locus_tag="PL056_13510"
     CDS             complement(5..253)
                     /locus_tag="PL056_13510"
                     /inference="COORDINATES: protein motif:HMM:NF013939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HPM48079.1"
                     /translation="MNNGINGLREKIDRIDDLIVELVSQRIEIASKIIELKKDSSMNV
                     EDISRENSIIKRLSGRSPEIGKLIEEIYGRIFNWVKSR"
     gene            complement(250..1422)
                     /locus_tag="PL056_13515"
     CDS             complement(250..1422)
                     /locus_tag="PL056_13515"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48080.1"
                     /translation="MKRTDLSNFIPADKSSLIRGYLFSLLTDEPVSVYYSDDLPNDIK
                     TAFKCLKKFGKTVTVSGSTAYISGVSTAPSESIDCENSATVMHILMGIAKYREWDLKL
                     TGDRSLMRRDHSEFTRAGELYGACFVETVLSKESAQLKSFHLIAMLKSGGKLHFKSKT
                     RRNTEELLKKMGAELFEKKGCIEVKPVKKLNGYELGMKLDPSSAFIAACSAIICGKQF
                     IISAIYPEKLRMEPFKYLKKAGYSVSVSLKDEGYEVKWSGGFKTGMKMIVVSNKEVPK
                     IIDEIPFIAYMSAKAGCSFELNNGEWLRNKETDRISETVKRMEMFFETVETEKGFIVK
                     GEKRAKTVKFSHSDDHRMEMLSKLIAVDKKIPFTVNDCYNVSFPGFLRMIEALGEK"
     gene            complement(1409..2419)
                     /gene="aroF"
                     /locus_tag="PL056_13520"
     CDS             complement(1409..2419)
                     /gene="aroF"
                     /locus_tag="PL056_13520"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HPM48081.1"
                     /translation="MIIVLKKNIPEENLEQIKSTLISNGMSFDVLTGVNYTLIPVAGD
                     LTTSDMGRFKAMPGIHKIIRISKPYFMVSKEFCDKSVIDLGDGVKIGEGLSFISGPCS
                     VEGPDSLDKIAKFLSSLGVKILRGGTYKLRTSPYSFQGLGEQGLKILKDVSSRYGMKC
                     ISEIVDVRHLDLFLEYTDIIQVGARNMMNFSLLKEIGRVKKPVLLKRSLSATVDDFLS
                     SAEYLMSEGNEKVVLCERGIQTFDDITRSTVNIASIPVLKDLSHLPVVFDPSHSIGAS
                     KYVPVTAEAAVILGADGLMVETHYNPTNALSDGFQTINFDSFEKMYKKISRLSSFLNE
                     EN"
BASE COUNT          718 a          559 c          409 g          796 t
ORIGIN      
        1 gagatcagcg gctttttacc caattgaaga ttctcccgta gatctcttct ataagttttc
       61 cgatttcagg agatctgccg ctaagtcgct taattattga attttctctt gaaatatctt
      121 caacattcat tgaactgtcc tttttgagtt ctatgatctt tgaagctatc tcgatcctct
      181 ggcttacaag ttcaacgatc agatcgtcga tcctgtcaat cttctccctt aacccgttta
      241 ttccgttatt catttttctc caagtgcttc gatcattctt aaaaaaccgg gaaacgaaac
      301 attatagcag tcattaactg taaaaggaat ctttttatca acagcaataa gttttgagag
      361 catctccatt ctgtggtcat cgctatgaga aaattttaca gtttttgctc tcttttctcc
      421 cttcacaata aaaccttttt cagtttcaac tgtttcaaaa aacatctcca ttctcttaac
      481 agtttcacta attctgtcgg tctctttatt tctcaaccac tctccgttat taagctcaaa
      541 actgcatccg gcctttgccg acatatatgc aataaatgga atttcatcaa taattttagg
      601 aacttctttg tttgacacaa caatcatttt cattcctgtt ttaaatccgc cggaccattt
      661 gacctcataa ccttcatctt ttaaacttac agaaactgaa tatcctgctt tttttaaata
      721 tttaaacggt tccattctca atttttcagg atatattgct gaaataataa actgtttacc
      781 gcaaattata gctgaacatg ccgcaatgaa ggcacttgac ggatcgagtt tcatgcccag
      841 ctcatagcca ttaagctttt ttaccggctt aacttcgata catccttttt tctcaaaaag
      901 ttccgccccc atttttttca gcagttcttc ggtgttgcgt ctggtctttg acttaaaatg
      961 tagtttccct ccactcttaa gcatcgcaat aagatgaaaa cttttgagct gggcactctc
     1021 ttttgaaaga actgtttcaa caaaacatgc tccgtaaagt tcacccgccc tagtaaattc
     1081 tgaatgatca cggcgcatta atgatctatc gccagtgagt ttcaaatccc attctctgta
     1141 tttagcaatc cccatcagaa tatgcatgac agttgcagaa ttttcgcagt ctattgactc
     1201 tgacggagca gtcgaaacac ctgatatgta tgctgtactg ccggaaacag tcacggtttt
     1261 accgaatttt ttcaggcact taaaggccgt ttttatgtca tttggcaaat catcgctata
     1321 atagacagaa accggctcgt cagtcagaag agaaaaaaga tatcccctta tcagtgaact
     1381 tttatcagcc ggaatgaaat ttgagagatc agttctcttc attgaggaat gatgacagtc
     1441 ttgatatttt cttgtacatc ttttcgaaac tgtcaaagtt gatcgtctgg aaaccgtcag
     1501 acagagcgtt ggtaggattg tagtgagttt ccaccatcaa cccgtcagct ccgaggatca
     1561 ctgccgcctc ggctgtcaca ggaacatatt ttgaagcacc tatactgtga gacggatcaa
     1621 aaacaacagg aagatgtgaa aggtccttga gcacagggat cgaagcaatg ttgaccgtac
     1681 ttcttgttat atcatcaaaa gtctggatac ccctttcaca gaggacaacc ttttcatttc
     1741 cttccgacat gagatattcc gcacttgaaa gaaaatcgtc aacggtcgca ctcagtgatc
     1801 tttttagcaa aaccggtttt ttaacccttc cgatctcttt taaaagtgag aaattcatca
     1861 tattccttgc cccgacctga ataatgtcgg tatattcaag aaaaaggtca agatgcctga
     1921 catcaactat ctctgaaata cacttcatcc cgtaacggct gctgacatct tttaatattt
     1981 tcaaaccctg ttcaccaagt ccctgaaaag aataggggga agttctgagc ttatatgtcc
     2041 ctccgcgaag tatctttaca cccagactgg aaagaaattt tgcaatttta tcaagagaat
     2101 cagggccttc tacgctacag ggacctgaaa tgaaagaaag accttcacca attttcacgc
     2161 cgtctccaag atcaataacg ctcttgtcgc agaattcttt agacaccatg aaataaggtt
     2221 ttgaaatcct tattatttta tgaataccag gcattgcctt gaacctgccc atatcagatg
     2281 tggtcaagtc accggcaacg gggatcaatg tgtaatttac acctgtcaga acatcaaaag
     2341 acatcccgtt tgatatcagt gtcgatttta tctgttcaag attttcttca ggaatgtttt
     2401 ttttaagaac aatgatcatc taataacctc ctaaatcgtt cgattttcaa taaaaaactt
     2461 gacaaaaacc tcctaaagtg aa
//
LOCUS       DAOOYB010000369         2474 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_52966_length_2474_cov_3.869781, whole genome shotgun sequence.
ACCESSION   DAOOYB010000369 DAOOYB010000000
VERSION     DAOOYB010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52966_length_2474_cov_3.869781"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="PL056_13525"
     CDS             complement(<1..413)
                     /locus_tag="PL056_13525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48082.1"
                     /translation="MKVFKIACALIFIIVSSASCDKNGDRYPDKDGNNVSDSDDLIDN
                     DPDLDLSDIDNPVIDSDIVTEEDVDTDIDIESDFDSSSDGDETDDESDENPDVDDVYV
                     PICGNGISEIGEECDKGVLNSDEPGEIGITCRTDCT"
     gene            complement(434..2371)
                     /locus_tag="PL056_13530"
     CDS             complement(434..2371)
                     /locus_tag="PL056_13530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48083.1"
                     /translation="MKSLILYFSLFVIFSSCVSKGGDNFYSYPDSDYYKSPDNNGKTQ
                     NDMENEKPDVSDINNDMESSDPDDFSDDDAFFDGDDRLDYSADESYYDDDQVFIDDAE
                     SDNDSEIFPECGNGLIENNEKCDTKDKVLCSAIDFVNYESGEASCKGDCSDWIVTDCV
                     EYENCADKPDFPDPDFADTNCDGIDGDIKLSVFVDMYSGDDLNDGSINSPLRSISKGI
                     IVASEAGKANVLVSTGSYENEEISFKDGVSVHGGYSGVPAWTRTSDSLSVTKILSDSE
                     GLIADTVSNVNLSFLKIQSLDTAGNGESSVALTLKSCTGISLSNLVLLAGKGSDGAAG
                     VNGLAGDKGGNGGPGNPGCESSTALFCDGPCSQPSGGAGGSSSCGAYGGTGGTPGKSK
                     SEGNPGENGSGSDSNGGTGGTLPVMGEEACYFSSGLDIHGGKGADGANGASGSGGLEI
                     GDFVDGQYYSADGKNGVDGANGQGGGGGGGGMGGKSYCDSYGSSGGGGGAGGCGGKAG
                     TGGQGGGGSFALIIDDSTSILLENIVLESNNGGNGGAAGRGGTLGQYGFGASGGNYGG
                     GGDQDDGGCGGHGGNGGLGGIGGHGGGGGGGPSICFVRDSYSLVEGEKTLSCKYGAGG
                     EGGPSSGNKGKNGFSAKSYIY"
BASE COUNT          709 a          703 c          376 g          686 t
ORIGIN      
        1 gtgcaatcag ttctacatgt aataccaatc tcgcccggtt cgtcagaatt taaaacgcct
       61 ttatcacact cctcacctat ctcagatatt ccattgccgc aaattggaac ataaacatcg
      121 tcaacatcag gattttcatc actttcgtca tctgtttcat caccatctga actactgtca
      181 aaatcagatt cgatatcaat gtcggtatca acatcttctt cagttactat atccgaatca
      241 ataacagggt tatcaatatc agaaagatcc agatcaggat cattgtcaat cagatcatct
      301 gaatcagaaa cattgttccc gtctttatcg ggatatctgt ccccgttttt atcacatgaa
      361 gcagaactga caattataaa gattaaagca cacgctattt taaagacctt cattaaaccc
      421 ctcctgtctc cgattaatat atataacttt tagcactgaa accattcttg cctttgttcc
      481 cggaactagg gcctccttct cctccggcac catatttaca cgacaaagtt ttttctcctt
      541 caactagcga atatgaatct ctgacaaaac agatggatgg accgccaccg ccaccgccgc
      601 catgacctcc aatgccgccg agtccaccat ttccaccatg tcctccacat cctccatcat
      661 cctgatcacc gccaccaccg tagtttccgc cacttgcacc aaacccatac tgtcccaatg
      721 ttcctccacg acccgcagct ccgccatttc ctccattatt tgattcaaga actatgtttt
      781 ccaataaaat actcgtagaa tcatcgataa tcaaagcaaa acttccacca ccaccttgtc
      841 ccccggtgcc cgcttttccg ccacaaccgc cggctccgcc accgccaccg gaactaccgt
      901 aactgtcaca atatgatttt cctcccattc cgccgccgcc accgccaccg ccttgaccgt
      961 tggctccatc aaccccgttt ttgccatcag cagaataata ttgtccgtca acgaaatcac
     1021 ctatctctaa accacctgaa ccgcttgcac cattagctcc atctgccccc tttccgccat
     1081 gaatatccag tcctgatgaa aaataacacg cctcttcacc cataacaggc agagttcctc
     1141 ctgtccctcc gttgctatct gaacccgaac cgttttctcc cggattccct tcactttttg
     1201 atttgcctgg tgttcctccg gtaccgccgt atgctccaca agaagaacta ccgccagctc
     1261 cgcctgaggg ttgagaacat ggtccatcac aaaataatgc ggtggaactc tcgcaaccgg
     1321 gatttccagg acctccattt ccgcccttgt caccggcaag accattaacg ccggcagctc
     1381 cgtcagaacc ttttccagcc aaaagcacca gattcgacaa agaaattccc gtacaggact
     1441 tcaaagtaag agcaacggaa ctttcaccgt ttccggcagt atcaagactc tgaattttta
     1501 gaaaagataa atttacattt gaaacagtat ctgcaataag tccttctgaa tctgacaaga
     1561 tttttgttac cgacaaagaa tcggatgttc ttgtccaggc aggaactccc gaataccctc
     1621 catgcacaga aacaccatct ttgaatgaga tctcctcatt ctcgtaggat cctgtcgaaa
     1681 caagaacatt tgctttcccg gcttctgaag cgacaataat accttttgaa attgatctta
     1741 gcggtgaatt aatcgaaccg tcgttaagat catctccaga atacatatca acaaaaacag
     1801 atagtttgat gtcaccatcg atcccgtcac aatttgtgtc cgcaaagtcc gggtcgggaa
     1861 aatccggttt gtctgcacaa ttttcgtact caacacaatc tgtaacaatc cagtcggaac
     1921 aatcaccttt acaggatgct tctccacttt cataattaac aaaatcaatg gcactgcaca
     1981 gtaccttatc tttcgtatcg catttttcgt tattctcaat aagaccgttt ccacattccg
     2041 gaaatatttc agaatcatta tcagactctg catcatcaat aaaaacttga tcatcgtcat
     2101 aataactttc atctgctgag taatccagac gatcatctcc atcgaagaat gcatcatcgt
     2161 cagaaaaatc atccggatca gagctttcca tgtcattatt tatatcactc acatccggtt
     2221 tttcgttttc catatcattc tgtgtttttc cgttattatc gggtgactta taataatcac
     2281 tgtcagggta agaatagaaa ttgtcacctc ccttggaaac acaggaagaa aaaatcacaa
     2341 acaatgaaaa atataaaatt aaactcttca cacagacctc ataagcagat tttaaacaaa
     2401 ctttctattt tgcacaacgg aatcctcttg ctgactgacg cagattcgga taatgcttgc
     2461 ttcttaccga agta
//
LOCUS       DAOOYB010000370         2472 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_53015_length_2472_cov_12.551510, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000370 DAOOYB010000000
VERSION     DAOOYB010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53015_length_2472_cov_12.551510"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            138..326
                     /locus_tag="PL056_13535"
     CDS             138..326
                     /locus_tag="PL056_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015778053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPM48084.1"
                     /translation="MKYRIIEGLCINCGACAAECPCEAVIQNEKEYQITVSLCIGCGQ
                     CAEVCPVDCIEKIDEQGE"
     gene            328..795
                     /locus_tag="PL056_13540"
     CDS             328..795
                     /locus_tag="PL056_13540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48085.1"
                     /translation="MIKRWIFLIFMLVFSVGCENSVNSLKNKSDDEKNDEKTVVDEEN
                     DPGVVQDEGAKEPDESEETPDEQVDENENQDVDFIWDNCSNTWDCEGDEMCVKEIGRC
                     TDSWGKCEKIPEDCEAVDEPVCGCDGMSYANKCIAMQNAQNIKHNGVCETGLK"
     gene            792..1247
                     /gene="rimI"
                     /locus_tag="PL056_13545"
     CDS             792..1247
                     /gene="rimI"
                     /locus_tag="PL056_13545"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HPM48086.1"
                     /translation="MILSKKQTESLKNSIISIGLQYFGDDTGSFLDSYISAIDTQYII
                     FCHLENGLPEGFLILSEVLDEAEIIQVAVRKESTGRGIGTQLVKEVAEYCKSNGVRKI
                     FLEVRVSNRSAEKIYLKNGFEKVGMRRSYYSNPVEDALIMAKSCCGWCQ"
     gene            1244..>2472
                     /locus_tag="PL056_13550"
     CDS             1244..>2472
                     /locus_tag="PL056_13550"
                     /inference="COORDINATES: protein motif:HMM:NF017808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPM48087.1"
                     /translation="MKRQLVVALLIVSVISSCYGRKGDGVRKSIKTWTLHAESNALFC
                     GDIFFQNELKGDLYVVAVKEPDAPNLPRTVLNYKKIEKGTFYGFLVPVGSVSVFAFQD
                     RNGNKKYDDDELFGTYKGGEPVFLDNGRMLFRVDITLDNVQNMPEKFRKMSIVNDVKS
                     DYVVKKPFPGTVVKMDHYYFDPGFGALGMWRPLELASLQGPSVFMLKEFDPDKIPILF
                     VHGISGTPFDWKFISENIDTEKYLPMVFQYPSGFRLDGVAAALDYEISVISEKYGMKE
                     IIIVAHSMGGLVSRTFIQRYHNQKENSLVRKFISISSPYGGHDLAAYGVRKETKSFVP
                     VWIDMVPGSDFQKRMFETPLNVPFYLFYGNKPKDGQKNSDSDGTVSVKSMLDENVTKD
                     AVEIFQFHEDHTSILNS"
BASE COUNT          799 a          409 c          616 g          648 t
ORIGIN      
        1 ctgaaaagca ggtgtcgatc tttgaagcgc agcagcagat cgaagctgaa gcaaagaaaa
       61 ataaaccgaa acagaaaaaa gaaaaaatag taaaaaagaa atgatccggt acaattttca
      121 agtggagcgg ggattaaatg aaatacagaa taattgaagg tctatgcatt aactgcggag
      181 cttgtgccgc agaatgtccg tgtgaagcgg tgatacagaa tgaaaaggag taccagataa
      241 cggtctctct ttgtatcgga tgcggtcaat gcgcagaggt ttgtccagtg gattgtattg
      301 agaaaattga tgaacagggg gaataaaatg atcaagagat ggatcttttt aatttttatg
      361 ttagtttttt cagttggttg cgagaattct gtcaattcac tgaagaacaa gtctgatgac
      421 gaaaaaaacg atgaaaagac tgttgttgat gaggaaaatg acccgggcgt tgttcaggat
      481 gagggcgcga aagaacctga cgagagtgaa gaaactcccg atgaacaggt tgatgagaac
      541 gaaaaccagg atgttgattt tatatgggat aactgcagta atacatggga ttgtgaaggt
      601 gacgaaatgt gtgtcaaaga aatcggcaga tgcactgatt cctggggaaa atgtgaaaaa
      661 atcccggagg attgtgaagc agttgatgaa ccggtctgcg gatgtgacgg aatgtcctat
      721 gccaataaat gcatcgctat gcagaatgct cagaacatta aacataacgg tgtgtgtgaa
      781 accggactga aatgatcctt tcaaaaaaac agacagaatc tttgaaaaac tcaataatat
      841 caatcggttt acagtatttt ggagatgaca ccggttcttt tcttgacagt tacatttccg
      901 caattgacac acagtacatc atattctgtc atttggaaaa cggcttgccg gaagggtttc
      961 tcatcctttc agaagtgctt gatgaagcag agataattca ggtggctgta agaaaagaga
     1021 gcacaggcag gggaatcggg acgcagctgg ttaaagaggt tgctgagtac tgcaaatcga
     1081 acggtgtcag gaaaatattt cttgaagtaa gggtttcaaa cagatcagcg gaaaagatct
     1141 atctgaagaa cggttttgaa aaggtgggta tgagaagatc gtattacagc aatcctgttg
     1201 aggacgcttt aattatggca aaatcatgtt gcgggtggtg tcaatgaaaa gacaactggt
     1261 cgtagcattg ttaatagtgt cagtaatatc atcgtgttac ggcagaaaag gcgacggggt
     1321 gagaaagagt ataaagacat ggacgctcca tgctgaatcc aatgctttgt tctgcgggga
     1381 tatatttttt caaaatgagt taaaaggcga tctgtatgtc gtggccgtga aagaaccgga
     1441 tgctcccaat ctgccgagaa cagtgctcaa ttataaaaag atcgaaaaag ggactttcta
     1501 cggttttctt gtgccggtcg gaagcgtttc ggtctttgct ttccaggata ggaacggaaa
     1561 taaaaaatat gatgacgatg aactgttcgg aacatataaa gggggagaac cggtttttct
     1621 ggataacgga cggatgcttt tcagggtcga cataactctt gataatgttc agaatatgcc
     1681 ggaaaagttc aggaaaatga gcatagttaa tgatgtgaaa agtgattatg ttgttaaaaa
     1741 accgtttccc gggacagttg tgaaaatgga ccattattat tttgatccgg ggttcggagc
     1801 actcggtatg tggagacctc ttgagctcgc atctttgcag ggaccgtcag ttttcatgct
     1861 gaaagaattt gatcctgata aaattccgat tctttttgtt cacggaatat cgggaactcc
     1921 ttttgactgg aaattcatat ctgaaaatat cgacacggaa aaatatctcc ccatggtttt
     1981 ccagtatccg agcggattcc ggcttgacgg tgttgctgcg gcacttgatt acgaaatctc
     2041 ggtcatttcg gaaaaatacg gaatgaagga gataataatt gtcgctcaca gcatgggagg
     2101 acttgtttca agaactttta ttcaaagata tcacaatcag aaggagaact cccttgtcag
     2161 aaaattcatt tcgataagtt caccttacgg cgggcacgac cttgccgcat acggagtcag
     2221 aaaagagaca aaatccttcg ttcctgtatg gatagacatg gttcctggat cagatttcca
     2281 gaaaagaatg tttgaaacac ctttgaatgt ccctttctat cttttttacg gaaataaacc
     2341 caaagacgga cagaagaaca gcgacagcga cggaacagtc agtgtgaaaa gtatgcttga
     2401 tgagaatgtt acaaaagatg ccgttgaaat atttcagttt cacgaagatc acacctcaat
     2461 acttaattca ga
//
LOCUS       DAOOYB010000371         2462 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_53329_length_2462_cov_14.316992, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000371 DAOOYB010000000
VERSION     DAOOYB010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53329_length_2462_cov_14.316992"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="PL056_13555"
     CDS             <1..183
                     /locus_tag="PL056_13555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48088.1"
                     /translation="IPAKKNIFFHFTIESYDNMGLVSTLAKENGDLILECSTPMSNAK
                     FFDPLIDKILKEVMSD"
     gene            176..754
                     /locus_tag="PL056_13560"
     CDS             176..754
                     /locus_tag="PL056_13560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48089.1"
                     /translation="MISRFLKSAVFIAVLFFCSSLTALIEGRIAGNRISSLSAEISLE
                     KSEFLYNAPLLQYFNLNFGFMVNRFQNPGLSFGVDFQWLDAEKAAGTMRFNGIFLTNR
                     TFSDIILGGYVDASLVAGAKISGFKGVVHSIVTATPGSDPLVGLEFQLGAYVSPHESV
                     NFFLAPAFGKYFVKSDSFYFNFILSLEVFLWK"
     gene            751..>2462
                     /locus_tag="PL056_13565"
     CDS             751..>2462
                     /locus_tag="PL056_13565"
                     /inference="COORDINATES: protein motif:HMM:TIGR03960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HPM48090.1"
                     /translation="MITSVLRNINKPGRYFAPFIEPVRIESDPSKPKVLILFPDIFEM
                     AQSHTGVKILYSIFHRSGFNVDFGFAPDKDFIEIAKKEGTLRSMMHDIDFRHFDLICV
                     SFQYQLQYPAFLKILELAGIPVTSKERSGSNMPVIIAGGPVMCNPEPVSDFLDAAFIG
                     EFEPASGEVLKVLLQVKEREKIIEEFSKIEGFYIPDGSLSGTVVRRSILSDLNYDPDS
                     LFDAPVFAMRTIHDRFTIEIMRGCTRGCRFCMAGSFYRPHREKSAKNICGILDRVVKT
                     SGYDEAGFLSLSAADHSEIEEILVESYGKYSRGISISLPSLRTETLTSKIVSAIRKGR
                     KSGFTLAPESGSERLRKIINKDNTNEEIIEAVRKIFSNGWQNVKLYFMLGLPLENDED
                     IVETVDLVKKICSLARGFGKKAGVTASFSTFIPQPFTPFQWEKMATPEEIKAKQHIIL
                     DGLRNIKNLKLSWHSQEISTVEGYLSRAGREFNNVIRYVAERMEGLQTDDENFNSKLW
                     NDALLKNGIDPYTTMIEKPVDKPLPYEHIDMRIDRSFLLREREKGYNSERTADCADGN
                     CENCG"
BASE COUNT          725 a          463 c          551 g          723 t
ORIGIN      
        1 atccctgcaa aaaagaacat ttttttccat tttacgattg aaagttatga taatatgggt
       61 cttgtttcaa cacttgcaaa agagaatggt gatctaatcc ttgaatgttc cacaccgatg
      121 agcaatgcaa aattctttga tccgcttatc gataagatat taaaagaggt aatgagtgat
      181 tagccgtttt cttaaatctg cggtttttat cgcagtgctg tttttttgca gttcgttaac
      241 ggcacttatt gaaggccgta tagcaggaaa cagaatttcc tctctgagtg ctgaaatttc
      301 gcttgaaaaa tcggagtttt tatataacgc tcctttattg cagtatttta accttaattt
      361 cggttttatg gtcaaccgct ttcagaaccc agggctttct ttcggtgtcg attttcagtg
      421 gcttgatgca gaaaaagcgg caggaacgat gaggtttaac gggatattcc ttactaacag
      481 gacattttca gacataattt taggcggata tgttgacgct tctttagttg ccggagcaaa
      541 gatatcggga tttaaaggag ttgttcattc catagttact gcaactcccg gaagtgatcc
      601 gctggtaggt cttgaatttc agcttggcgc ctatgtttcg ccgcatgagt cggtgaattt
      661 tttcttagcg cctgcttttg gaaaatattt tgttaagtca gacagttttt atttcaattt
      721 cattttatct cttgaggtgt tcctgtggaa atgatcacat ccgtattaag aaatatcaat
      781 aaacccggaa gatatttcgc cccttttatt gagccggttc gcatcgagag cgatccttca
      841 aagccgaaag tactcatcct ttttcccgat atttttgaga tggcacagag tcataccggt
      901 gtaaaaatac tttattcaat ttttcatcgc agcggtttta atgtcgattt cggttttgct
      961 cctgacaaag attttattga aattgccaaa aaggaaggga cgctgagatc gatgatgcac
     1021 gatattgact tcagacattt cgacctgatc tgtgtttctt ttcagtatca gctccagtac
     1081 cccgcttttt taaagatact tgagcttgca ggcattcctg taacttcaaa ggaaaggtcg
     1141 ggcagcaata tgccggtgat cattgccggc ggaccggtca tgtgtaatcc tgagccggtc
     1201 agtgattttc tggatgccgc tttcatcggt gaattcgaac cggcatcagg tgaagttcta
     1261 aaagttcttc ttcaggtgaa agagagagag aagataattg aagagttttc aaaaattgaa
     1321 ggattttata ttccggacgg ttcgctttcc ggaacagttg tcagaagatc aattctgagc
     1381 gatcttaact atgatcctga ttctctgttt gatgcacctg tttttgcaat gagaacgatt
     1441 cacgatagat ttaccatcga gatcatgcgt ggctgtacaa gaggttgcag gttctgtatg
     1501 gcgggatcat tctaccgtcc tcatcgtgaa aaaagtgcga agaacatttg cggcatcctt
     1561 gacagagtag taaaaacatc aggatatgat gaagccggct ttttatctct ttctgcggca
     1621 gatcacagtg aaatagagga gatacttgtc gaatcatacg gaaaatacag tcgcggaatt
     1681 tcaatatctc ttccgagttt aagaaccgaa acactcactt caaaaatagt ttctgcaata
     1741 agaaaaggga gaaagagcgg atttacactt gctcctgaat ctggcagcga acggctcagg
     1801 aagatcataa acaaggataa caccaatgaa gagatcattg aagcagtcag aaagatcttc
     1861 agcaacggct ggcagaatgt aaagctctat ttcatgctcg gacttccgct tgagaatgat
     1921 gaagatattg tcgaaaccgt cgaccttgta aaaaaaatat gttcgcttgc cagaggtttc
     1981 gggaaaaaag cgggcgtgac ggcaagtttc tcgacattca ttccgcaacc gttcacccct
     2041 tttcaatggg agaaaatggc aactcctgaa gagataaagg caaaacagca tataattctt
     2101 gacggactta gaaatataaa aaaccttaaa ctcagttggc acagtcagga aataagcaca
     2161 gtcgaagggt atttaagccg ggctggcaga gaattcaaca atgttatcag atatgtggca
     2221 gaaaggatgg aagggttgca gaccgatgat gaaaatttta attcaaaact ctggaatgat
     2281 gcacttttga aaaacgggat agatccgtac accacaatga ttgaaaaacc ggttgataag
     2341 ccgctccctt atgaacatat tgacatgaga attgacagat cgttcctgct tagagaaaga
     2401 gaaaagggct ataattccga acgcactgcc gattgtgcag acggtaattg tgaaaactgc
     2461 gg
//
LOCUS       DAOOYB010000372         2458 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_53457_length_2458_cov_6.967957, whole genome shotgun sequence.
ACCESSION   DAOOYB010000372 DAOOYB010000000
VERSION     DAOOYB010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53457_length_2458_cov_6.967957"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..616)
                     /locus_tag="PL056_13570"
                     /pseudo
     CDS             complement(68..616)
                     /locus_tag="PL056_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011187442.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
     gene            complement(634..1071)
                     /locus_tag="PL056_13575"
     CDS             complement(634..1071)
                     /locus_tag="PL056_13575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGGC domain-containing protein"
                     /protein_id="HPM48091.1"
                     /translation="MKIGIIICERYKSCAGGKCFRAMNCREGAFDIYDKNESLEIAGY
                     TGCGGCPGGNIEYAPVEMIKNGAEVIHLATGFVVGYPPCSYTAYFKKYIEEKFKIKVV
                     VGTHPIPQNYYITHHNLSTWQTDDWEELIRPTLNDECLRKKYD"
     gene            complement(1385..2278)
                     /locus_tag="PL056_13580"
     CDS             complement(1385..2278)
                     /locus_tag="PL056_13580"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPM48092.1"
                     /translation="MTKFDRFKLVHLLPFAVVFLGGFIDNEKVLYCISTTANYLFGNI
                     RLSQRALLYLCVFVSSSIYIIFSLKEVRLFYGRAKILQSSSIRLKRNWQIIFLCYAVN
                     ILILYVFSTVVYLANSKYTGIVSFLFCVYIYTPVFFAGYAVLIKGDYFSIFHKSSCEE
                     NISTEEDKDFNIKYARSRLSESKKDDYLTRILCSLDQDKVYLDPDLTMQSFSLITGIP
                     LYHISQVVNLKLGVNYNTLINARRINHAKSTLADKSSMNRTVLEIAFESGFNSKTTFN
                     NLFRAETGLTPTEFRRRAEKE"
BASE COUNT          859 a          484 c          356 g          759 t
ORIGIN      
        1 ctttttctaa accattattt tttaattaat tgcacttctc gattgaagtg gtgtcacaca
       61 gttcactttc tatatcatta taacctggaa gaataaaaac ttccaggtac attttccctc
      121 tatattcttt tctgaaagaa actaagccat caatgtattt atgaaaatcc aaatcacttg
      181 ctggacggtt tatttttttg aaacattctt cagttgcggc gtcaagagaa ggcagaacaa
      241 tatccgcatc aagaatttcg tttcttacat cggcatcaaa caataaagtc ccatttgtca
      301 aaacagctac aggaatctcc ggtttatttc gtttaataaa ttccagtatt tcaccaatga
      361 atacatttaa agccggctct cccgaacctg agaaagttat atagtccgga tcagaattgt
      421 tcgcgaagta atgattcaat tcttcaatta ttttatcacg cttaacatat tcctttctct
      481 caagagtaag tttcgtcgtt ttgccgacct cgcagtagac acagtcaagg gagcacacct
      541 ttgacggaac aagatcaact ccgagcgaca ttcctaagcg tctcgacgga accggtccaa
      601 atatattcct atacatattt tgccttaaac cgttcagtca tatttttttc ttaaacattc
      661 atcattcagc gtcggtctta tcaattcctc ccaatcatct gtctgccagg ttgaaagatt
      721 gtgatgagta atataatagt tttgaggaat cggatgagta ccgacaacaa cttttatttt
      781 aaatttctct tcaatatatt tcttaaaata agctgtataa gaacatggcg gatatccgac
      841 aacaaatccc gtcgcaaggt gaattacttc agcaccattt tttatcatct caacaggagc
      901 atactcgata tttccgcccg ggcatccccc gcaacccgta tatccggcaa tttccaatga
      961 ttcatttttg tcataaatat caaacgcacc ttctcggcag ttcatcgctc tgaaacattt
     1021 tcctccagca cagcttttat atcgttcaca tattataata cctattttca taaaaattct
     1081 ccacataaat tactaaacaa taacaaaatc atttctattc gtatgaccat attaaaataa
     1141 tctaaaataa ttaaagtcaa gtttattcgc atttagaaga gataacagaa tgaatgattt
     1201 ttcatcacct tctgtagcag attagcatat attgcctcat cacacaaatt ttgctcttat
     1261 aaaggctgtc gagaatataa tataagctta ctgagaaaaa atataactta tgcctatttc
     1321 caactcgcaa gtcattgccg aaaaaagtaa aactggaata atcaaagact gacgaatgag
     1381 accgctactc tttttcagct cttctgcgga attctgtagg agttaaaccc gtttccgccc
     1441 tgaacagatt attgaaagtt gttttggaat taaatccgct ctcgaaggct atttcaagaa
     1501 ctgtcctatt catacttgat ttatccgcaa gagtactctt tgcatgatta attcttctgg
     1561 cgttaatcaa tgtattataa tttacaccaa gttttaaatt tacaacctga gaaatgtgat
     1621 acaaaggaat tccggttatt aacgaaaaag actgcattgt aagatccgga tcaagatata
     1681 ctttatcttg atcaagagaa caaagtatcc gggtaagata atcatctttt ttgctttcag
     1741 acaatctgct tctggcatac ttaatattga aatctttatc ttcttcagtg gaaatatttt
     1801 cttcgcatga cgatttatga aaaatactga aatagtctcc ttttatcaaa acggcatatc
     1861 cggcaaaaaa aacaggggta taaatataaa cgcaaaataa aaaagaaact atacctgtat
     1921 acttcgaatt agcgagatat accaccgtgg agaacacata cagaatcaga atattcacag
     1981 cataacaaag aaatattatc tgccaattgc gctttaatct tattgatgat gactgtaata
     2041 tttttgcccg tccataaaac aacctgactt cttttaatga aaaaataata tatatagagg
     2101 aggaaacaaa gacgcacaaa tacaataagg ctctttgcga aagacgaata tttccgaata
     2161 aataatttgc agttgtactt atacaatata atactttctc attatcaata aaaccaccca
     2221 agaatacaac ggcaaaagga agtaaatgaa ccagcttaaa tcggtcaaac ttcgtcattc
     2281 tgcccgaaat agaaagaata taaaaataga acaaaggacc ataaaggaag aaaaacgaat
     2341 ctctaatttc aaatagaatc acataatcat tatacccatg ctttcggaaa aagaactgca
     2401 taaatatcac cacttcaatc gagaagtgca attaattaaa aaataatggt ttagaaaa
//
LOCUS       DAOOYB010000373         2436 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_54189_length_2436_cov_9.702646, whole genome shotgun sequence.
ACCESSION   DAOOYB010000373 DAOOYB010000000
VERSION     DAOOYB010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54189_length_2436_cov_9.702646"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1158
                     /locus_tag="PL056_13585"
     CDS             <1..1158
                     /locus_tag="PL056_13585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48093.1"
                     /translation="DEPGTGRKMYSSGPIKKTGTSENVTVTAGKFYIFGVAWVNPMAS
                     YYATYDGIFNPFVSQITTFGKTLGGTAITDSYPLLPTIPGTNKGLTNYNMYFNTGNTS
                     TEICICNNLCPALNNKRCNNNWIENCAADTYGCLSWEAVTDCDYKDCETDNGVPECVN
                     NCVNECTLNNKQCNGNEIEKCTTNAVHGCAEWTFDLDCSISMITPYCGKDGENSAKCY
                     EVPQVSQEYIASSGVTFFNASDDYFKGMYIQATHNAVITGFKIHLQQPEGETGVTIPF
                     SVYEGSTESGPYTRIYRSTHVVGSNGYYGPTGINISVTAGKYYLLAVYMPYNTGYYYV
                     NGISTALTYDLKFGTSIGNESFATSTEPVAAYTFDGPYLSSYRMWIESYLN"
     assembly_gap    1231..1330
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1331..1503)
                     /locus_tag="PL056_13590"
     CDS             complement(<1331..1503)
                     /locus_tag="PL056_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HPM48094.1"
                     /translation="MAKYRIAWLPGDGVGKEVMEAARIVLDGLKLNAEYIHGDIGWEF
                     WCKEGDPLPKRTT"
     gene            1667..2341
                     /gene="pyrE"
                     /locus_tag="PL056_13595"
     CDS             1667..2341
                     /gene="pyrE"
                     /locus_tag="PL056_13595"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008425171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HPM48095.1"
                     /translation="MKNYKHDFIDFMVECEVLMFGDFVTKSGRKTPFFINTGKYRTGE
                     QIRKLGQFYAQSIMENTNGEFDVMFGPAYKGIPLCVTTAIALSEMYNKDAAFCFNRKE
                     AKDHGEGGSIIGHKLKDGDRVVIIEDVTTAGTSIAETVPILKAAANVDVNALVISVNR
                     MERGTGEKSALDEIRETYGMKTFPIVSMAEVTEYLYNKEIKGKILLDDKIKSMIEDYY
                     KLYGAR"
BASE COUNT          748 a          493 c          478 g          617 t
ORIGIN      
        1 gatgaaccgg gaacaggaag gaaaatgtac agctccggac cgataaaaaa gacaggtact
       61 tcggaaaatg tcactgtaac agcaggtaaa ttctatattt tcggtgttgc atgggttaat
      121 ccaatggctt cctattatgc aacatatgac ggtattttca atccattcgt ttctcagata
      181 acaactttcg gtaaaactct tggcggaaca gctataacag attcatatcc gcttctgccg
      241 acaattccgg gaacgaacaa aggacttacg aactataata tgtactttaa cacagggaac
      301 accagtaccg aaatatgtat ctgcaacaat ctctgtcccg ctctaaataa caaaagatgc
      361 aacaataact ggatagaaaa ctgtgcggca gacacttacg gctgtctttc atgggaagct
      421 gttactgatt gtgattacaa agactgcgaa accgacaacg gagtccctga atgtgtcaac
      481 aactgtgtca atgaatgtac tctcaataac aagcagtgta acggaaatga aatagaaaaa
      541 tgtacgacaa atgcagttca cggctgcgcg gaatggactt ttgatcttga ctgttccatc
      601 tccatgataa ctccgtactg cggtaaagac ggagaaaaca gtgcaaaatg ttacgaagtt
      661 ccgcaggttt ctcaggaata tatcgcctca agcggtgtaa ctttcttcaa tgcatctgac
      721 gattatttca agggaatgta tattcaggca acacacaatg ccgttatcac cggttttaaa
      781 attcaccttc agcaacccga aggtgaaaca ggcgtaacca ttcctttttc tgtatatgaa
      841 ggaagcaccg agtcaggacc gtacacaaga atttacagaa gcacacatgt tgtcggatct
      901 aacggatact acggaccgac aggcataaat atcagcgtca cagccggcaa atattatctt
      961 ctggcagttt atatgccata caacaccgga tattattatg taaacggcat ttcaaccgca
     1021 cttacatatg acctgaagtt cggaacttct ataggaaatg aatcattcgc aacttcaact
     1081 gaaccggttg ccgcatacac ttttgacggt ccctatcttt caagctatcg catgtggatt
     1141 gaaagttatc tgaattaatt caaggattta tttaagggca ttcttaagaa cttcgtgagc
     1201 cacttcaaga gttttcgctt ttccgccaag nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn tcggttgttc ttttgggcaa tggatcaccc tctttgcacc agaattccca
     1381 gccgatatct ccgtgaatat attcggcatt taatttaaga ccgtcaagaa cgatccttgc
     1441 ggcttccata acttccttac caacgccgtc acccggcagc catgcgatac ggtactttgc
     1501 catgtccttc tccttttgaa gttgaaatat ctgaaaactc tccacagtta atgaacttta
     1561 cgccctgaga ttctatcgta tgaattttaa gtgtcaaaaa aattgaaaac cagtcaaagt
     1621 taaagtattt tcaacacaga aaatttcatt cttaacggag gataaaatga aaaactacaa
     1681 acatgatttc attgatttta tggttgaatg cgaagttctt atgttcggag actttgtgac
     1741 aaagagcgga aggaagaccc cttttttcat aaacaccgga aaatacagga caggtgaaca
     1801 gatcagaaag ctcggtcagt tctatgccca gtcaattatg gaaaacacga acggtgagtt
     1861 cgatgtcatg ttcggtcctg catacaaagg aatccctctt tgtgtcacga ctgccattgc
     1921 actgagcgaa atgtataata aagacgccgc tttctgcttc aaccgcaaag aggcgaaaga
     1981 tcacggtgaa gggggctcta tcatcggtca caagcttaaa gacggcgaca gagttgtaat
     2041 tatcgaagat gtgacaacgg caggaacgag catcgctgaa actgttccga tactcaaagc
     2101 ggcggcaaat gtcgatgtca atgcacttgt catttctgta aaccgcatgg aacggggtac
     2161 tggtgaaaaa agtgcactcg atgaaataag ggaaacttac ggaatgaaaa cattcccgat
     2221 cgtttcaatg gcggaagtaa ctgaatatct ttacaataaa gagatcaaag ggaaaattct
     2281 tcttgatgac aaaatcaaat caatgatcga agattactac aagctttacg gagcaaggta
     2341 acaacatcaa agatctcttt caaatccaag tgattttaaa agccggatat catcgtccgg
     2401 agttattcct ttaacggtaa gaagctgtcc tgtgat
//
LOCUS       DAOOYB010000374         2407 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_55135_length_2407_cov_5.350765, whole genome shotgun sequence.
ACCESSION   DAOOYB010000374 DAOOYB010000000
VERSION     DAOOYB010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55135_length_2407_cov_5.350765"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            362..>2407
                     /locus_tag="PL056_13600"
     CDS             362..>2407
                     /locus_tag="PL056_13600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48096.1"
                     /translation="MKKIFVVLIFWGFSIFGQENIFFYPTGEMQNPVENHILYRVNVN
                     INPSVNFQNGVIGKEWVEIELLDSLGVPFTLTLSRTKEMTFSKGPVESLWFGVVDDGV
                     NRNGKVMNSYIARTVNGKYYSRVYYDNGLYVMFPENVVTGSPVLPSTVYHLQKIDPLL
                     MPEEEDLHYSLQDEFSEEEPVQIGTHYNAGTDYEINGLIGIAPEVYEYTNYTLEQIAM
                     KMTGDLNSTFINTGINNVKITLQFENLQCPDSDPDCTNCTSGGFFCEICSNSNLLSNP
                     GDFEDNDSKETCQSTYRWNYIEMWQDPCTDQDCVNKNRKQSYCGGALDGNSTPNPYVG
                     HLHRCPSESGKGLSEYVGAGFTGIVTYYRFVQRNSDGSAKPDLNGDGSYWRHSLISRA
                     GSIMDKYIISHEFGHILNIQHTAESRARSNLFSCNGCSCEVKEKTNLDGTHELGWDCA
                     GTCDTGKCQDDIKSRFNEIITPGNIYYNPEKYLDPNFSGKGRYYIHSYANISGGYKFY
                     TVTGKKTEFLDAANNFFERHINAFSRSDIHYCDTSLINPVCGQGGDPVYADAYHFLTQ
                     PSTTQTVPGNPVTTITINNITVIDDLATRYKLVCPVKEKWCADNDRDGACDDRLTVIE
                     VCRGDDPNDSGTNTNYISDNGTIDNCRNNYNSVVSYSLKDELISGENGAIIKRLGRLG
                     "
BASE COUNT          782 a          417 c          501 g          707 t
ORIGIN      
        1 ttcggttgaa ccgcaactgg ctgaaaacaa taagaaaaca aaaattgtta ttgtgtagag
       61 tgctctcatc tgatcctccg ttatcaaaat gaatttaacg cgattagttt atcactttaa
      121 atatttttta caaatttgtg ttgacatctt aaaaacaatt taatacaact taatcagata
      181 tcggttggaa gcaaaaatga caaaaaattc aaataaagct tgctttccct gttttaacgg
      241 gatgattgtc acaagacaag acaagacaag acaatccttt aattttttta gatatattca
      301 gaatgtttca agagtttttc acgggatatt tttattattt tttcttgggg gggggcgtgt
      361 gatgaagaag atttttgttg ttttaatctt ttggggattt tcaattttcg ggcaggagaa
      421 tattttcttt tatcccacag gagaaatgca gaatcctgtt gaaaatcaca tattgtacag
      481 ggtgaatgtt aatattaatc catctgtaaa ctttcaaaac ggtgtgattg gaaaagaatg
      541 ggttgaaata gagttgctgg atagcttagg agttccgttc accttaactc ttagcagaac
      601 aaaagaaatg acattcagca agggaccggt ggaatcattg tggttcggtg ttgttgatga
      661 cggggtaaac cgtaacggca aagtcatgaa ttcttatatt gccaggaccg tcaatggaaa
      721 atattactcc agagtatatt acgacaacgg tctttatgta atgtttccgg aaaatgtagt
      781 aactggatct cccgttttac cctccaccgt ttatcatctt cagaagattg atccgcttct
      841 catgccggaa gaggaagatc tccactattc tttacaggat gaattctctg aagaagagcc
      901 ggttcagata ggaactcatt acaatgcagg aacggattat gaaattaacg gattgatcgg
      961 cattgctcct gaagtatatg aatatacaaa ttacactctt gaacagattg caatgaaaat
     1021 gacaggcgat ctcaacagca cctttatcaa cacaggtata aacaatgtaa aaattacact
     1081 tcagtttgaa aatcttcaat gccctgactc agatccggat tgcacaaatt gtacttcagg
     1141 gggattcttt tgtgaaattt gttctaattc caatctgttg tcaaatcccg gtgatttcga
     1201 agacaacgac agcaaggaaa cttgtcaaag cacctataga tggaactaca tcgaaatgtg
     1261 gcaggatccc tgcactgatc aagactgtgt taataaaaac aggaaacaga gttattgcgg
     1321 aggagcattg gatggcaact caactccaaa tccgtatgtg ggacatcttc atcgctgtcc
     1381 atcggaatca ggaaaaggac tttcagaata tgtgggagcc ggatttaccg gaattgtcac
     1441 ctactacaga tttgttcagc ggaattcaga tggttctgca aaacccgatt tgaacggaga
     1501 tggctcttac tggagacatt cgctgatttc aagagccggt tcgataatgg acaaatatat
     1561 tatttcacat gaattcggac atattctcaa tattcagcat acagctgaat cgagggcaag
     1621 atctaacctt ttttcgtgta acgggtgctc ttgtgaagtt aaggaaaaaa caaatttaga
     1681 cggcacccat gaattgggat gggattgtgc gggcacatgt gatactggta aatgtcaaga
     1741 tgacataaaa agcagattca acgagataat aactccgggc aatatatatt ataatcctga
     1801 aaaatatctc gatcccaatt tcagtgggaa agggcgttat tacattcatt cctatgcaaa
     1861 tatttcaggc ggttataaat tttatactgt aaccgggaaa aagactgagt ttttggatgc
     1921 agcgaacaat ttttttgaaa gacatataaa cgcattctcc aggtctgata tccattactg
     1981 tgatacaagt ttaatcaatc ctgtttgcgg acagggaggc gatcctgttt atgctgatgc
     2041 atatcatttt cttacacaac cgtctacgac tcagacagtg ccaggaaacc cggtaacaac
     2101 cataactata aacaatataa ctgtaataga tgatcttgca accagataca aacttgtatg
     2161 tcctgtaaaa gagaagtggt gtgcagacaa tgatagagat ggcgcatgtg atgacaggtt
     2221 gacagtaatt gaagtctgcc gtggtgacga tcccaatgat tcagggacaa atacaaatta
     2281 tatttctgac aacggcacaa ttgacaattg ccggaataac tataattctg ttgtttccta
     2341 cagtcttaaa gatgaattga tttctggaga aaacggtgca attataaaaa gattgggaag
     2401 attggga
//
LOCUS       DAOOYB010000375         2406 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_55154_length_2406_cov_9.397703, whole genome shotgun sequence.
ACCESSION   DAOOYB010000375 DAOOYB010000000
VERSION     DAOOYB010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55154_length_2406_cov_9.397703"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(394..1386)
                     /locus_tag="PL056_13605"
     CDS             complement(394..1386)
                     /locus_tag="PL056_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HPM48097.1"
                     /translation="MTGGIITFWIIVGIFAVTILVKGVRIVPQAMVIVIERLGKYHKT
                     LQSGFNLIIPIIDKPREMDIKTMRQMYDGRKMTVTSRVSLIDMREAVHDFPKQNVITK
                     DNVVIEIDAILYYQITDPFKAMYEISNLPDAIEKLAKTTLRNIIGNMDLDHTLSSRDE
                     INSKMTDILDEATDKWGVKVNRVEIQDINPPQDIKNAMEKQMRAERDKRAAILTAEGD
                     KQSKILESEGEKQKNINYAEGMKESNILEAEGHAQAKIRVAQAEAEAIRQIADAIKET
                     KMDPAQYLVALKYIEALSAMTSGKDNKVVYMPYEASGILGSVGSIQEMFKNLPK"
     gene            complement(1411..1872)
                     /locus_tag="PL056_13610"
     CDS             complement(1411..1872)
                     /locus_tag="PL056_13610"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HPM48098.1"
                     /translation="MEFSTIDIWILWGIAAVICFIFEIFLPSFWMAMLGVGAIGASLS
                     ALLGGGTEIQIAVFSFVSIIAGVFFRPLAYKYIYNSNEKKAANVDALIGRKVQVVSKI
                     TTEKNGKVKIGSEVWTAISENRSESFNEGAFVTVASVDGAKVIVKSSASEN"
     gene            complement(1888..2289)
                     /locus_tag="PL056_13615"
     CDS             complement(1888..2289)
                     /locus_tag="PL056_13615"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HPM48099.1"
                     /translation="MKAGVIWKETTIFEGFNDKNSEKNFVKSDGPTPKHLFLQSIAGC
                     TAIDVVGILEKMHSEMPTEFVVSVEGDLTENHPKVFKNFIMVYDVRGNVDPAKLKRAV
                     KLSQENYCGISTMAKKIAPFEIKVVLNGNEI"
BASE COUNT          644 a          487 c          433 g          842 t
ORIGIN      
        1 gataaattat cctccaagat gcttaatatc agtgaaataa tatggaaaag ttgatatttg
       61 tcaatataat gtatctctag tttatttgtt ttaaataaat gattgatgtt tcacaaagaa
      121 tacgaaatga aacacactag atatgggaat ttatcaataa ttacaaaaag caaaggtttt
      181 tttcaaaact ctcttgacaa acaaaaaata aacagttata acttttcatg ttcaattcta
      241 atgctgcttt aaatgagggg cgcataatgg aaggcaaagt ggcacaggca accgttgagg
      301 ttgtatcttt agattaattt cttctcaact ttttcttctt tgttcagaac ctgccttgcc
      361 cctcaatctt ttttttgaat ttttatgatg aatttatttg gggagattct taaacatttc
      421 ctgaattgaa ccgactgagc cgagaattcc gcttgcttcg taaggcatat agacaacttt
      481 attgtctttt cctgaagtca ttgctgacag agcttcaata tatttaaggg ctacgagata
      541 ttgtgcaggg tccattttgg tctcttttat tgcatcagct atctgtctga tcgcttcggc
      601 ttccgcctgg gcaactctga tctttgcctg agcatgacct tctgcttcaa gtatattcga
      661 ttctttcata ccttcggcat agttgatgtt tttctgtttt tcaccttcag attcaaggat
      721 ctttgattgt ttgtctcctt cagcggtaag aattgccgct cttttgtctc tttctgccct
      781 catctgtttt tccatcgcat ttttgatgtc ctgcggtgga tttatgtcct gaatttcgac
      841 acggttgacc tttacacccc atttgtccgt agcttcatca agtatatctg tcatttttga
      901 gtttatctca tccctgcttg acagtgtatg gtcaaggtcc atgttgccga tgatgtttct
      961 aagagtcgtt tttgcaagtt tttcaatggc gtccggaagg tttgagatct catacattgc
     1021 cttgaatgga tcggttatct gatagtagag tattgcgtcg atctcgatga cgacattgtc
     1081 ttttgtaata acattctgtt tcggaaaatc atgaacagct tctctcatat cgatcaagct
     1141 gactcttgat gtaacggtca tttttcttcc gtcatacatc tgacgcattg ttttgatgtc
     1201 catttctctg ggtttatcaa ttatgggaat gataaggtta aatccgctct gaagggtttt
     1261 gtgatacttg ccgagtcgct cgatgacaat aaccattgcc tgaggaacga ttcttacacc
     1321 tttaacgagg atcgtaacag caaatatgcc gacaatgatc cagaaagtga taattccgcc
     1381 tgtcattttc tactccgttc ttttttgtta ttaattctca gaagcacttg attttactat
     1441 tacttttgct ccatcaacag aagccacggt tacaaaggcc ccttcattga agctttctga
     1501 acggttttca gatatggctg tccatacttc gctgcctatt ttcacttttc cgttcttttc
     1561 ggtggttatt tttgaaacga cctgaacttt gcgacctatc aatgcatcaa cattggcagc
     1621 ttttttttcg ttactgttgt agatatactt atatgcaaga ggccggaaaa aaacaccggc
     1681 gattattgaa acaaaggaaa aaacagctat ctggatctcg gttccccctc ctaataaagc
     1741 cgagagagat gctcctattg cgccgacacc cagcattgcc atccagaagc ttggaagaaa
     1801 aatttcaaaa atgaaacata ttacagcggc aatcccccaa aggatccaga tgtctattgt
     1861 agaaaattcc atggtacctc ctgattttta tatttcattt ccgttaagca caacttttat
     1921 ttcaaatggg gctatctttt ttgccattgt agaaattccg caataatttt cctgtgagag
     1981 cttcacagct cttttcagtt tggccggatc aacatttccc cttacatcat agaccataat
     2041 aaagttcttg aatactttcg ggtgattctc tgtcagatcg ccttctactg aaacaacgaa
     2101 ttctgtcggc atttcagagt gcattttctc aagaattcca acaacatcga tagcggtgca
     2161 tccagcaatt gattgaagaa aaaggtgctt gggcgtaggt ccgtcacttt ttacaaagtt
     2221 cttttcactg tttttatcgt tgaatccttc aaagatcgtt gtttctttcc agatgactcc
     2281 cgctttcatg aactttctcc attgaaattt gacaacatgg attatatcaa aataaaaatc
     2341 tgttgcaagt gtaataaaaa agaaagggtg ttgttactaa aaccctgttc aatgctcaat
     2401 gaaaaa
//
LOCUS       DAOOYB010000376         2400 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_55348_length_2400_cov_9.635394, whole genome shotgun sequence.
ACCESSION   DAOOYB010000376 DAOOYB010000000
VERSION     DAOOYB010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55348_length_2400_cov_9.635394"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1391)
                     /locus_tag="PL056_13620"
     CDS             complement(<1..1391)
                     /locus_tag="PL056_13620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48100.1"
                     /translation="MKKLLMVTLLLLVASAFVSCNSYDVEGKPEVASNPAKKVFAYYP
                     WDSNDVQQPDDTETPDDTEITDETETPDETETPDEAETPDEAETPDEEESDDDTVNPV
                     MPVLKAPVADTITEEFEIYNRGETGELLIRQINLIDPRGHRIAELDNADYKKLFELQI
                     QSMSNTGVATDGWNGKLLTFDNTNAEAGSRIGSLCPLDQNEIEKRTCKKSFSAQKYNP
                     QFKILLSYNKTAAEALKLNPPSEKEYNPVTGEDDLKYKLDGDFWIEICTNDPTKGTSD
                     TCGPNATSYRIQVTRQPNKPPKPIIKVKFEYTTGGEMSYRNIKDKVQMNLVNTCVSDP
                     DAPSKCLDKKLYDKKDPNRRYAIKYKWEMKESPTPLMEESQIKTAGKWILDIDDPERE
                     DFYGLMITPRRYSEKNPNYKDATTCESECGEEPKDVEDEFYFLKLSNYLICRQKHCEK
                     FKTKYYKVNIQAET"
     gene            1604..>2400
                     /locus_tag="PL056_13625"
     CDS             1604..>2400
                     /locus_tag="PL056_13625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48101.1"
                     /translation="MALSQNGWIICLEPERKGLLAGTKVHLTQCISCEVKECPVKAQL
                     FSAYFPQWVDLENSPLNGKIMELNRTLAGRKKRVHEFYCPKKKTASISCNARSVFCRY
                     NLICGKFDEAETGTGFIKSIRWRRVMIYVVMYLDGTSDVVNRNDFATVDIDRVERVYP
                     GSYEVKIVSELVPLGEEKEKLAETVESFNQKYKGGVVTANGLVNFDEWFTNGSNGDTA
                     VIPEKVLVPQKTYKVQKIKDTVEISESNGAAKIDKIPGKVEKVEKVE"
BASE COUNT          647 a          464 c          540 g          749 t
ORIGIN      
        1 gtttccgcct gaatattgac cttgtaatat tttgttttga atttttcaca atgtttctgg
       61 cggcagataa gatagttaga gagtttcagg aaatagaact catcttcaac atctttgggt
      121 tcttcaccgc attcgctttc gcaagtggtg gcatctttgt aatttggatt cttttctgaa
      181 tatcttctag gagttatcat aagtccatag aagtcttctc tttcaggatc atctatgtca
      241 agtatccact ttccggctgt ctttatctga gattcctcca taagcggagt gggtgattct
      301 ttcatttccc acttatattt tatagcatat ctacgattcg gatccttttt gtcatataat
      361 ttcttgtcaa gacattttga aggtgcatca ggatcggaaa cgcaggtgtt cacaagattc
      421 atctgaactt tgtctttgat gttcctgtat gacatttctc cgccagtggt gtattcaaat
      481 ttaactttaa ttatcggctt tggcggtttg ttcggctggc gggtcacctg aattctgtag
      541 cttgtagcgt taggcccgca tgtgtcggaa gttccctttg tcgggtcgtt cgtgcaaatc
      601 tcgatccaga aatctccgtc aagtttgtat ttcagatcat cttcgcctgt aacaggatta
      661 tattcttttt ctgagggagg attcaactta agggcttcag ccgcagtttt attgtagctc
      721 aggaggatct tgaactgtgg attatacttc tgtgcactga aacttttctt acaagttctc
      781 ttttcgatct cattctgatc aagaggacag agagatccga tgcgtgatcc tgcttcagcg
      841 tttgtgttgt caaaagtcag aagtttgcca ttccagccgt ctgttgcgac acctgtattt
      901 gacatggact gtatctgaag ctcaaacagc tttttataat cagcattgtc aagttctgct
      961 attctgtgtc ctctcgggtc gataagattg atctgtctta tcagaagttc acctgtttca
     1021 ccgcggttat atatctcaaa ctcttcggtg atagtgtctg caacaggagc tttcagaaca
     1081 ggcattaccg gattgacggt atcatcatca ctttcctctt cgtcaggagt ctcagcttca
     1141 tcgggagtct cagcttcatc gggagtttca gtttcatcag gggtttcagt ttcatcagtg
     1201 atctcagtgt cgtcaggagt ttcagtgtca tcgggctgct gaacatcatt gctgtcccac
     1261 gggtagtatg caaaaacttt ttttgcgggg tttgatgcca cttccggctt tccttcaaca
     1321 tcgtaactgt tgcaggaaac gaaagctgaa gcaaccagca acagcagtgt aaccataagt
     1381 aatttcttca tttctcaatc ctccaaataa tattataaag agtaacacct catcaaaact
     1441 tcagcgcatt ataaaatgac atagtcaaaa gtcaaaaaaa aagagcgctg tcgcatgtca
     1501 caaatgttta gtacgcacac aatgaaattt ttacgaaaaa tcttcttgtt tttaaaatga
     1561 tccgtgatta aactgttgcc gaaacttatt gccggtgtgt gttatggctt tgtctcagaa
     1621 cggatggata atatgtcttg aacctgaaag gaaaggtctt cttgcaggga caaaggttca
     1681 cttgacccag tgtatatcat gtgaagtgaa ggagtgtccc gttaaagcac agcttttttc
     1741 agcatacttc cctcagtggg tcgatcttga gaacagtcct ctgaacggta aaattatgga
     1801 gctgaacaga actcttgcag gcaggaagaa gagagtccat gaattctact gtccgaaaaa
     1861 gaagacggca tcgataagtt gtaacgcaag aagtgttttc tgcagataca atttgatctg
     1921 cgggaaattt gatgaagcag aaacaggaac cggttttata aaatccataa gatggaggag
     1981 agtcatgatt tatgtcgtaa tgtaccttga cggcacttca gatgtcgtta accgcaatga
     2041 ttttgcgaca gttgacattg acagggtcga aagggtctat cccggcagtt atgaagtaaa
     2101 gatcgtttca gagcttgttc cgctcggaga ggagaaggaa aagcttgctg aaacagtaga
     2161 atctttcaac cagaaataca agggcggagt tgtgacggca aacggtcttg taaattttga
     2221 tgagtggttc acaaacggct cgaacggtga caccgctgtt attcctgaga aggtgctggt
     2281 gcctcagaaa acatataagg ttcagaaaat aaaggataca gtagagattt ctgaaagtaa
     2341 tggtgcggca aaaatagata agatcccagg gaaggttgag aaggttgaga aagttgagaa
//
LOCUS       DAOOYB010000377         2384 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_55870_length_2384_cov_3.743237, whole genome shotgun sequence.
ACCESSION   DAOOYB010000377 DAOOYB010000000
VERSION     DAOOYB010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55870_length_2384_cov_3.743237"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="PL056_13630"
     CDS             complement(<1..220)
                     /locus_tag="PL056_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019598191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4172 domain-containing protein"
                     /protein_id="HPM48102.1"
                     /translation="MFIYENKNWPEFFWDSEKLLPLISEVRNFQGQIVGKMGSFGFDL
                     KNQANLEMLSLDVIKSTEIEGEILNKDQV"
     gene            complement(406..1020)
                     /locus_tag="PL056_13635"
     CDS             complement(406..1020)
                     /locus_tag="PL056_13635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48103.1"
                     /translation="MEKFDLKKVLKSLSKKRPVFHSEADFQFALAWEIKEEYKSAEIR
                     LEKRFETGKKNNYFDIFFELDGKKYAIELKYKTAKLEDKLYDEEYNLKGQSAQDCGRY
                     DFLKDVQRLEKLKESKKINQGFAIFLTNDHYYWSAGRKENSVDAKFRINEDRKIEKDS
                     TLEWTSDAADGTKKGRTDPIKLKKDYTCKWFKYSALFQYLLFEI"
     gene            complement(1099..1584)
                     /locus_tag="PL056_13640"
     CDS             complement(1099..1584)
                     /locus_tag="PL056_13640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48104.1"
                     /translation="MCENSRKDLVSLLPVTSFEKEFNAKINPGWLVISYRWASEDTKS
                     ERRRKYGRWYEIKSENGRIFRVMSFSGSIEGNYVNEKKCFIGDIVVDWAGWIELTGYS
                     GEEKKQIEVQIRQIRKWQYILCAFSHPDPNYRLSALLGLLSLFLGLLSLVFAIIPFIK
                     I"
     gene            complement(1669..>2384)
                     /locus_tag="PL056_13645"
     CDS             complement(1669..>2384)
                     /locus_tag="PL056_13645"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HPM48105.1"
                     /translation="WNGSTWIPSSTGTYNIVPSTSECRFSCIENHVWNGTQCVEGRIA
                     VCTAKPANTEWNDGDKNGTYIQTWNGSSWQPEIVTTYNTTEGDCQYVCLIGYYWEDNL
                     CRELQWSLKAPSEKTWSQAISYCNNLVESGHSDWRLPTISELRTLIQNCPATETSGEC
                     TVTDECLYYSSCRHTACDGCSPSGSDGRYSKLGDTEWFWSSSTQPDISTNAWYVLFRD
                     GKVSFHPKANSLSGMRCVR"
BASE COUNT          702 a          497 c          368 g          817 t
ORIGIN      
        1 ggacctgatc tttattaagt atctcccctt caatttccgt tgatttaata acatcgagtg
       61 aaagcatctc aagatttgcc tgatttttaa gatcaaaccc gaaagacccc attttcccaa
      121 cgatctgacc ctgaaaattt ctgacctctg aaattaaagg aagcagtttt tcactatccc
      181 agaaaaattc aggccagttt ttgttttcgt agataaacat gcccattctc cgcacaatct
      241 gcggagaata taccgcttaa tctccgcatt gtcaaggaaa agaacaagat catttaagtc
      301 aacgaaaaac aaaggaaaaa ggagttaatt tgatgagttg ggaaggatta aaggagaaaa
      361 aacatgtgtg ggaaagggga tttcttttgt tggtcttttg ctgttttaaa tctcaaacaa
      421 caggtattgg aataaggcac tatatttaaa ccatttgcag gtataatctt tttttaattt
      481 aataggatct gttctacctt ttttagttcc atcggcagca tctgaagtcc attctaatgt
      541 ggaatctttt tcaatctttc gatcttcatt aattctgaat ttagcatcta cagaattttc
      601 ctttctaccg gcactccaat aatagtgatc atttgtcaaa aagattgcaa aaccctgatt
      661 tatctttttt gattctttta atttttctag tctttgaaca tcttttaaaa aatcgtatct
      721 tccacaatct tgagctgatt gaccttttag attgtattct tcatcatata atttatcctc
      781 caatttagca gttttatatt ttaattcaat tgcatatttt tttccatcca gctcaaaaaa
      841 aatgtcaaag taattgttct tcttaccggt ttcaaacctt ttttccaacc tgatttcagc
      901 actcttgtat tcctctttta tttcccatgc taaagcaaat tggaaatcag cttcagaatg
      961 gaaaacgggt ctttttttcg acaacgattt aagaaccttc tttaaatcga atttctccat
     1021 aaccatttct ccttaattaa ttgtttatac agctctctga aatcaggatc tttgctatat
     1081 ttaatgatac ttttcaaatt atatttttat aaaaggaata atcgcaaaaa caagagatag
     1141 caacccaaga aacaaagaca acaaacctaa tagagcagat agtctataat ttggatcagg
     1201 atgagaaaat gcacacaaaa tatattgcca ttttcttatt tgtctaatct gaacttctat
     1261 ctgttttttt tcttcgcctg aataacctgt gagctctatc cagccagccc aatcaacaac
     1321 aatatcgcct ataaaacatt ttttttcatt aacatagttt ccttcaatac tgccagaaaa
     1381 actcataaca cgaaaaattc gaccattctc gctctttatc tcataccagc gtccatactt
     1441 ccttcttctt tcagattttg tgtcttctga cgcccatcta tatgaaatta caagccatcc
     1501 tggattaatt tttgcattga attctttctc aaaacttgtc accggcaata atgaaactaa
     1561 atcttttctt gaattttcac acataaaaca tctctatcaa attttatatt aaaagttgaa
     1621 tcctcaaatt cacttcaaca gttatttatc actatcattt tttttgtcct aacgcacaca
     1681 acgcataccg ctgaggctgt tggccttggg gtgaaagctc actttgccat ccctgaaaag
     1741 cacataccat gcattggttg aaatatctgg ctgagttgac gacgaccaga accattcggt
     1801 atctccaagt ttggaatatc tgccgtcaga tccggatggc gaacatccat cacaagcagt
     1861 gtgccggcaa ctgctataat aaagacactc atctgtcact gtacattctc cgctggtttc
     1921 tgttgcagga cagttttgga ttaaagttct aagctctgaa atggtgggaa gccgccaatc
     1981 tgaatggcca ctttctacaa ggttattgca gtaagaaatt gcctgactcc aggttttttc
     2041 tgagggggct tttaaagacc actgaagttc cctacagaga ttgtcttccc aatagtatcc
     2101 aattaaacag acatattggc agtctccttc tgttgtgttg tatgttgtca ctatttccgg
     2161 ctgccaggag cttccgttcc atgtttgaat gtaagttcca tttttatcac cgtcgttcca
     2221 ctcagtgttt gcaggtttgg cagtgcagac tgctatcctt ccttcaacac actgagtgcc
     2281 attccaaaca tggttttcta tacagctaaa tctacattct gaggtgcttg gaactatatt
     2341 gtaggttcct gttgatgaag gaatccatgt tgaaccattc catg
//
LOCUS       DAOOYB010000378         2376 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_56134_length_2376_cov_9.912107, whole genome shotgun sequence.
ACCESSION   DAOOYB010000378 DAOOYB010000000
VERSION     DAOOYB010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56134_length_2376_cov_9.912107"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="PL056_13650"
     CDS             complement(<1..252)
                     /locus_tag="PL056_13650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48106.1"
                     /translation="MKRILILLTMMVFVLVMASCETKKIEKNDETIDEDVIEVDTETD
                     NDSVTETDDDQVVPDEDVCGLPQDWLAPASEWEAWGYLRM"
     gene            complement(347..1381)
                     /locus_tag="PL056_13655"
     CDS             complement(347..1381)
                     /locus_tag="PL056_13655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48107.1"
                     /translation="MIKKTLMILVLFLVISCGEEIDPYKEYGNTGNTGDSGNTGNTGN
                     TGNSGNTGDSGDTGDTGNTGNTGNTGNTGDTGDTGIDWSTCEGIVTCTFGCADFDELC
                     ESFCYNDGSNEGQSDYRKWKECFENSCAETPTFECSEEKCPDESAICGVSADDPVPQP
                     FPAPYGTADISAEFTFIVNNGSPAGESELISGPFVTGKISKINIAPPQAGLIVSYVNY
                     SSSGDEDILEILQIPMLNESRIIINPVVMMTIKVADASKGVHSIGLDSATDAKLMLIN
                     IDEDGEITCHYAFAIGSFEINEAAIVPGEDGKLFLSGSGIELFSPRNIPAYGGDAQDY
                     LDVMSCSLIK"
     gene            complement(1396..1605)
                     /locus_tag="PL056_13660"
     CDS             complement(1396..1605)
                     /locus_tag="PL056_13660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48108.1"
                     /translation="MVKSKVGDLAKQKLFLVHFKDGYIKPVIAVSTDHAKKLIIEEKS
                     RKIEDIEKIVLEQNYYPQNEDYCDV"
     gene            1737..>2376
                     /locus_tag="PL056_13665"
     CDS             1737..>2376
                     /locus_tag="PL056_13665"
                     /inference="COORDINATES: protein motif:HMM:NF023169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HPM48109.1"
                     /translation="MIFFLLFTLLQVESFNSEKDFYILISSDDQNVTFSSFRLTNPDR
                     IVLEVSGSCSANELNPSLTVKEISILEKDGITRFVFNVAQGSHYTVLNRKNHILIGFS
                     PEIFIEDGVFDDLVANLRTQKQKEFENQHREIENQQKIAQLKLENEQKIKKETDESAL
                     VATMLKDLEEQKSREADEKKRIAKLELAKQEEIKLAEEKRIAEEKRIAEEKRI"
BASE COUNT          699 a          501 c          436 g          740 t
ORIGIN      
        1 cattctgaga tagccccatg cttcccattc tgatgcaggt gcaagccagt cctgaggaag
       61 gccacagaca tcttcgtccg gaaccacctg gtcatcatct gtctcagtaa cagaatcatt
      121 gtctgtctcc gtatctactt caattacatc ttcatcgatc gtttcatcat ttttttcgat
      181 cttcttggtt tcacaacttg ccatgacaag tacaaaaacc atcatggtca aaagaattaa
      241 aatccgtttc atcggcaccc tccgggataa taagttacta aatatctatt tggcgtgtaa
      301 attttacttt tatactgttt tttgtcaaga taaaaactac atttatttat tttatcagtg
      361 aacaggacat aacatcaaga taatcttgcg catctcctcc gtaagcaggg atatttctgg
      421 ggctgaaaag ctcgattccg gaacctgata gaaaaagttt cccgtcctca ccgggaacga
      481 tcgcggcttc attgatctca aaagatccta tcgcaaatgc ataatgacat gttatttcac
      541 catcttcatc aatgtttatg agcattaatt tcgcatctgt tgcggagtca agaccaattg
      601 agtgaactcc ttttgaagca tcggcaactt ttattgtcat cattaccaca ggatttatta
      661 taattctgct ttcattaagc atcggtatct gcaatatctc aagaatatct tcatccccgc
      721 tgcttgaata attaacataa cttacaataa gcccggcttg aggcggagca atgttgatct
      781 ttgagatctt tcccgttaca aaaggaccgg aaatcaactc cgattctcct gcaggcgagc
      841 cgttgttgac aatgaatgta aactcggctg aaatatctgc tgttccgtaa ggtgccggaa
      901 agggttgcgg aacaggatca tcagcggaaa ctccgcatat tgctgattca tcaggacatt
      961 tttcctccga acattcaaaa gtgggtgttt cagcgcaact attctcgaaa cattctttcc
     1021 acttacggta atcggactgc ccttcatttg atccgtcgtt atagcagaaa ctttcacaaa
     1081 gttcatcaaa atcggcacat ccgaaagtac atgttactat cccttcacat gttgaccagt
     1141 ctattcctgt atcacctgtg tcgccagtat ttccagtatt gccagtattt ccagtattgc
     1201 cggtgtcacc tgtatcacct gaatctccgg tattgccact gttaccggtg ttgccggtat
     1261 tgcctgtatt gccggaatca cctgtattgc ctgtgtttcc atattctttg tatggatcta
     1321 tttcctctcc gcaggaaata acaagaaaaa gcaccagaat catcagtgtt tttttaatca
     1381 ttggtcaccc ctatcctaca catcacaata atcttcgttc tgaggataat agttctgttc
     1441 caggactatt ttttctatgt cttctatttt tctcgatttt tcttcaatga taagtttctt
     1501 tgcatggtct gtggaaaccg ctattaccgg ctttatgtaa ccatccttga aatgaacgag
     1561 aaaaagtttc tgctttgcta aatcccctac tttgctttta accatatgag cgctccataa
     1621 ttaaaacgga ttattataat ccaaatctcc tgttgcagtc aagcaataat ggtaaaaact
     1681 ttgatttgta acttcagtat gtataatcac cgctgtttta tggattagga atttgtatga
     1741 ttttttttct tttattcaca cttcttcagg tagaaagctt taattctgaa aaagattttt
     1801 atattcttat cagctcagat gaccagaatg tcacattctc atctttccgt cttacaaacc
     1861 ctgacaggat cgtgctggaa gtgagcggct cgtgttcggc aaacgaatta aacccttcat
     1921 taactgttaa agagatctct atcctggaaa aagacgggat tacaagattt gttttcaatg
     1981 tcgcacaggg aagtcattat actgttctga acaggaagaa tcacattctt atcggattca
     2041 gccctgaaat atttattgaa gacggtgttt ttgacgatct tgttgcaaat cttagaactc
     2101 aaaaacaaaa agaatttgaa aatcaacatc gggagatcga aaaccaacag aagatcgcac
     2161 agttgaaact tgaaaatgag cagaaaatta agaaagaaac tgatgaatct gctcttgttg
     2221 ccacgatgct gaaagacctt gaagaacaga aaagtagaga agctgatgaa aaaaagagga
     2281 tagcaaaact tgagcttgca aaacaggaag agataaaact ggctgaagag aagcgtattg
     2341 ctgaagagaa gcgtattgct gaagagaagc gtattg
//
LOCUS       DAOOYB010000379         2317 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_58195_length_2317_cov_6.149425, whole genome shotgun sequence.
ACCESSION   DAOOYB010000379 DAOOYB010000000
VERSION     DAOOYB010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58195_length_2317_cov_6.149425"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="PL056_13670"
     CDS             complement(<1..685)
                     /locus_tag="PL056_13670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48110.1"
                     /translation="MTHTGNAKTVMDVLTYNGVMPVNDLDYDGLSTQLETILTTQSNN
                     DDSDGDGLNDYIETLGTLNILLPWEGSSPVEQDIFAEVDYFGKIIDGNMFYFYKNFEA
                     DIETRLRNSFNLHGNIRFHVDVDDYLGEMADNKTLKYSECGTGGDADTFYLEDERANH
                     FTSARTGIYTWVVAANKGKLYPTSGGRSCGSLGDPAFMITAGFHDGGGSLNRYTGTTI
                     HEYGHIFGLG"
     gene            complement(719..1732)
                     /locus_tag="PL056_13675"
     CDS             complement(719..1732)
                     /locus_tag="PL056_13675"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HPM48111.1"
                     /translation="MLEEHKVEIVVLIEEAHDNGARYLKACEVVGISLRTLERWKLEG
                     MKDKRKCSSKRVVRKLSPERREEVIAVCNSEKYRDRTPHEIVPMLLDEGRYIASVRTM
                     YRVLKEEKLLHLRNGSRRRKKRPKPPERKASEPNKVWCWDITWMPSLVKGMFYYAYVI
                     IDIFDRFIVGWTVHEEESEEHSKDLFESLSRGRNIVFEYLHADNGAPMIGTTLVTFLQ
                     SVDVKVSFSRPRTSNDNPFIESFFKTMKYTPGYPMRFKDISHARKWMADFVNWYNTQH
                     LHSSIGFVTPEQMRNGKALEIFRKRNEVMEQAKMENPECWGSRETIQWKTVDEVILNP
                     EKR"
     gene            complement(1732..2217)
                     /locus_tag="PL056_13680"
     CDS             complement(1732..2217)
                     /locus_tag="PL056_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011745526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPM48112.1"
                     /translation="MPYSSSIKRGILKRVLPPENKSIRSTSKETGICEQTIRNWIKES
                     KSGSLDDGSGEKSPRYLSNREKYHLLMESAKIPSEELGEFLRERGLHSEHLTLWDQEF
                     REMAEKSENRKDRKMKELEKRVRELEKELNRKEKALAEAAALLILKKKLDALMEDRED
                     G"
BASE COUNT          560 a          568 c          390 g          799 t
ORIGIN      
        1 gaccaagccc aaaaatgtgt ccatattcat gaattgttgt tcctgtatat ctatttaagc
       61 tgcctccgcc atcatgaaaa ccggcagtta tcataaaagc cggatctccc aaacttccgc
      121 aggatctgcc gccagaagta ggataaagtt tccctttatt cgcagccaca acccatgtat
      181 atattcctgt ccttgctgaa gtaaaatgat tggctctctc atcttcaaga taaaaagtat
      241 ctgcatcccc gccagttccg cactctgaat attttagtgt tttattgtca gccatttcgc
      301 caagataatc gtctacatcc acatgaaacc tgatatttcc atgaagatta aaagaatttc
      361 tgagtcttgt ttcaatatcc gcttcaaaat ttttataaaa ataaaacata tttccatcaa
      421 taatttttcc aaaatagtca acttctgcaa aaatatcttg ctctacagga cttgatccct
      481 cccatgggag aaggatattc agggttccga gtgtttcgat gtaatcgttc agcccgtcac
      541 cgtctgaatc atcattattg ctttgagttg ttaaaattgt ttcaagttga gttgataatc
      601 cgtcataatc cagatcgttt accggcatta ctccattata agtcagaaca tccataacag
      661 ttttggcatt accggtatgt gtcaaccttt ttgtcgcctt ttgttaaata atttttcttc
      721 atcttttttc cggattcaga atcacttcat ccaccgtttt ccactgtata gtttctcttg
      781 atccccagca ttccgggttt tccatttttg cctgttccat cacttcattt cttttcctga
      841 atatttccag tgctttgccg tttctcatct gttcaggtgt tacaaatcct attgatgagt
      901 gaagatgctg cgtgttatac cagttcacaa aatctgccat ccattttctt gcatgagata
      961 tatctttgaa tctcatagga tatccgggag tgtatttcat agttttgaaa aacgattcaa
     1021 tgaacggatt atcattgctg gttctgggtc tgctgaagga aactttcaca tctactgact
     1081 gcagaaatgt caccagtgtt gttcctatca tcggtgctcc gttgtctgcg tgaagatatt
     1141 caaaaacaat gttcctgcct cttgaaagac tttcgaaaag atccttgctg tgttcttcac
     1201 tttcttcttc atgaactgtc cagccgacta taaagcgatc gaatatgtca ataataacat
     1261 aggcataata aaacattcct ttcaccagtg aaggcatcca ggtgatgtcc cagcaccaca
     1321 ctttgttggg ctcagacgct ttcctttcag gcggtttggg tctctttttc cgtcttctgc
     1381 ttccgttcct taaatgaagc agtttttctt ctttaagaac tctgtacatt gtgcgtactg
     1441 aggcaatata ccgcccttca tcaagaagca tcggaactat ttcatgaggt gttctgtccc
     1501 tgtatttttc actgttacat actgcgatca cttcttctct cctctcaggc gacaactttc
     1561 taactacccg ttttgacgag catttccgct tgtctttcat cccttccagt ttccatctct
     1621 ccaatgtccg gagtgatatt ccgacaactt cacatgcctt caaatatctc gctccgttat
     1681 catgtgcctc ttctatcaga acaacaattt caactttgtg ttcctcaagg atcagccgtc
     1741 ctcgcgatcc tccataagag catcgagctt tttttttaag atcagcagtg cggccgcttc
     1801 cgcaagcgct ttctctttcc ggttaagctc cttttcaagc tctctgaccc tcttctcaag
     1861 ttctttcatt ttacggtctt ttctgttttc ggatttctct gccatctctc gaaactcctg
     1921 atcccacaag gttaaatgtt cactgtggag accccgttcc cttaaaaatt ctccaagctc
     1981 ctccgacggt attttcgccg actccatcag aagatgatac ttctcacggt tgctcagata
     2041 acggggactc ttctctccgc tgccgtcatc taagctacca gatttgctct cttttatcca
     2101 gtttcttatc gtctgttcac atatcccggt ctctttactc gtactcctga tgcttttgtt
     2161 ttcaggcggt aaaactcttt taagaattcc tcgtttgatc gaacttgaat acggcatgtc
     2221 ttcctcctaa gtttgaggcg acaattatat tagcacatac tgcgagcgag aatttcacta
     2281 aactgagtgt agatggtttg gatgagaaga aatttgt
//
LOCUS       DAOOYB010000380         2309 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_58499_length_2309_cov_7.635315, whole genome shotgun sequence.
ACCESSION   DAOOYB010000380 DAOOYB010000000
VERSION     DAOOYB010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58499_length_2309_cov_7.635315"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            124..1932
                     /locus_tag="PL056_13685"
     CDS             124..1932
                     /locus_tag="PL056_13685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48113.1"
                     /translation="MKNLTKSLILNTLLLFSVSALPISNFPVMEYEGAFDYFIISKSL
                     LLDTADHSDYEPQGDTSIGVTGASDFLLDTDVPADAVIEKAILVWFASVNDADKMALT
                     DNSVTLTIPDGSEHSVTASLQGNTASMQGFEFESYYKSGYYYYVYRVDVTDIIREMQY
                     DINSGEIKPMTGEYNVKGVDDIYDCQLSGTNHNYCKMASMIGGWQLIFVYGSSQIARK
                     RIYMYNGMDWSSNTNMNPTTINITNFELPENAAVKISFVTSDGDDYLPAPESLELQGG
                     LAPSALVLGEIGDQSCNPLNQPFNSKFRTINYKGEQSECRTELSFDIDTYFLQYDPEV
                     PDSIFNPHLQYGTNSMNFYIKTGADIVLTNYVILSVDTRLPAFDIPQKNEKFILTPLS
                     QSDKVCPKTIFGYQIVVENHGHEPAESVMVKDIVSGIQSYIPGTFQIDETGTGTCFKD
                     FEDNGGFPLEAGLKISESFNICQNEADCDRVLLRFLVKPVDEAPKNASFTNSASIWDA
                     KTGEGQSYKTNQGLPVRATFDNTCEPMDEKKVKNMLYKPESMACGGEVEIPDDSGDTG
                     DSANDDTDTGNTGDTSSDDGNITDEVEYVGCGCSVI"
     gene            1999..>2309
                     /locus_tag="PL056_13690"
     CDS             1999..>2309
                     /locus_tag="PL056_13690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48114.1"
                     /translation="MFTKMKLNKLIKKIQNRQRQMGWIKKKRNAIPEPIGKRLVSNLE
                     LTEELIRKHQTGHAKLEDAQKLLYDIDHNFKLHFDKFKQNALAELFDELWLVVLIALV
                     L"
BASE COUNT          709 a          502 c          485 g          613 t
ORIGIN      
        1 aatagctgtc gaagattctt cgacactgca tatctccttc tattcagttg aagaactgac
       61 agattcttcg ggtaaagcta ctgtcacaat atttgaaaaa gttgaataaa gcgagggtca
      121 aaaatgaaaa atcttacaaa gtcacttatt ctcaacacat tgttattatt ctcagtttca
      181 gctctgccca taagcaactt ccctgtcatg gaatatgaag gggcttttga ttatttcatc
      241 atctctaaat ctcttcttct tgacacggcc gatcattcag attacgaacc gcagggagac
      301 acttcgatag gagtaacagg tgcttcggat tttcttcttg acaccgatgt tcctgctgat
      361 gctgtgatcg aaaaagcgat acttgtctgg tttgcttcgg ttaacgatgc agacaaaatg
      421 gctttaaccg acaattcagt gacactcacg attccggacg gcagcgaaca cagtgtaacc
      481 gcttcgttac agggaaatac cgcttcaatg caggggttcg agtttgaatc atactataaa
      541 agcggatatt actactatgt ttacagagtt gatgtgaccg atattatcag ggaaatgcag
      601 tatgacatca attcgggcga aataaagccc atgaccggcg aatacaatgt taagggcgtg
      661 gatgatatct atgactgtca gttgagcgga acaaatcaca attactgcaa gatggcaagc
      721 atgatcggcg gctggcagct tattttcgtt tacggttcat cacagatcgc aagaaaaaga
      781 atttacatgt ataacggcat ggactggagt tcgaatacca atatgaatcc gacaactatc
      841 aacatcacca actttgaact tcctgaaaat gccgctgtca agatctcttt cgtgacaagt
      901 gacggagatg actatctgcc tgctcccgaa tctcttgaac ttcagggagg tcttgcacct
      961 tcagctcttg ttctgggtga gatcggtgat cagtcatgca atccgctcaa tcagccgttt
     1021 aacagcaagt tcagaacaat aaactacaaa ggggaacaat cagaatgcag gactgaactc
     1081 tcttttgata ttgataccta ttttctccag tacgatcctg aagttccgga ttccattttc
     1141 aatccgcatc ttcagtacgg cacaaattca atgaatttct acatcaaaac aggtgctgat
     1201 attgtcctca caaactatgt catcctgtct gttgatacac ggcttcccgc ttttgacatt
     1261 ccgcagaaaa atgaaaaatt catactgaca cccctctccc agagtgacaa agtgtgtccc
     1321 aaaaccattt tcggatatca gatagttgtc gaaaatcacg gccacgagcc ggctgaatcg
     1381 gtcatggtca aagacatcgt ttcaggaatt cagtcatata ttccgggcac tttccagata
     1441 gatgaaaccg gaacgggaac atgtttcaag gacttcgaag acaatggcgg atttcctctt
     1501 gaagcaggac ttaagatctc agaatcattt aatatctgtc aaaatgaggc agattgtgac
     1561 agagttcttt taaggtttct tgtcaagcca gtcgacgagg caccgaaaaa tgcctcattt
     1621 accaattcag cttccatctg ggatgcaaaa acaggcgaag gacaatcata caaaaccaat
     1681 cagggacttc ctgtgagagc cacttttgac aacacctgtg aaccgatgga cgaaaagaag
     1741 gtgaaaaaca tgctctacaa acccgaatcc atggcatgcg gcggagaagt tgaaattcct
     1801 gacgattcag gcgataccgg cgacagcgct aacgatgaca ccgacacagg caacaccggt
     1861 gacacctctt cagacgacgg taacattact gatgaagtcg agtatgtcgg ttgcggctgt
     1921 tcagtgatct gagtctttat attatcttgc aattcagaca atttcggtta ttttttaccg
     1981 tacttattgg aggcggtgat gtttacaaaa atgaaactca acaagctgat taaaaagatc
     2041 cagaatagac aaaggcagat gggctggata aagaagaaac ggaacgctat tccagaaccg
     2101 attggaaagc gccttgtttc aaatcttgaa ttaactgaag aacttataag aaagcaccag
     2161 acaggacacg cgaagctcga agatgcacag aaactgcttt atgacataga tcacaatttc
     2221 aagctccatt ttgacaaatt caaacagaac gcacttgccg agctttttga tgaactgtgg
     2281 cttgttgttc tcattgcact tgtacttaa
//
LOCUS       DAOOYB010000381         2289 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_59263_length_2289_cov_12.920770, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000381 DAOOYB010000000
VERSION     DAOOYB010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59263_length_2289_cov_12.920770"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            142..432
                     /locus_tag="PL056_13695"
     CDS             142..432
                     /locus_tag="PL056_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HPM48115.1"
                     /translation="MRSSEEIKKILNQNMEHMKNEFSVSKIGIFGSYVRDSARPDSDI
                     DIIVEFEEKSFDNYMDLKFFLEDLLGKKVDLVVSDSIKKRAKPTILKEAEYV"
     gene            425..775
                     /locus_tag="PL056_13700"
     CDS             425..775
                     /locus_tag="PL056_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006512396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HPM48116.1"
                     /translation="MYRDPILYLEDILDAIRKIREYIAEITFEDFCDDEKTVDAVIRN
                     LEVIGEAAKNLPQNMKDIHPEIEWRKITAIRNIVVHQYFGVDNFIIWDVISTKLGSLY
                     DAATDLLNKPQQFS"
     gene            complement(856..1725)
                     /locus_tag="PL056_13705"
     CDS             complement(856..1725)
                     /locus_tag="PL056_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HPM48117.1"
                     /translation="MAENELIQIDDIKSRIFTIRGMQVMLDRDIATFYSVKTIKLREQ
                     VKRNIKRFPSDFMFQLTEIEADFMVSQNAIPSKRHLGGFLPYVFTEQGVASVSSVITS
                     EKAIETSVLIMRAFVAMRHFLAGKADLFKRVDFLEIKQLEADSKFKDLFKALESNQLL
                     PKQGIFFEGQVFEAHKFVSDIVRSAEKSIILIDNYVDDTVLTLFSKRKQGVTLRILTK
                     NLSKQLQLDTQKFNEQFPPAEIKEFSYSHDRFMIIDNRDLYHFGGSLKDLGKKWFGFS
                     KMDVEVTKILTKI"
     gene            complement(1842..>2289)
                     /locus_tag="PL056_13710"
     CDS             complement(1842..>2289)
                     /locus_tag="PL056_13710"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HPM48118.1"
                     /translation="PVSEGLSYIHSALVLFFKDRYLEMLAETDSIFIIFPVWWGVMPA
                     ILKGFFDKVFLPKRAYIYKKSGMLKPLFPQIKQAVIFNTLGGPKIYHNLMLFFPVKKV
                     VADTTLKIIGVKKVKIVQFDRMKKSTDELRAKWLDQVEKSVGKMVK"
BASE COUNT          745 a          418 c          394 g          732 t
ORIGIN      
        1 cgaaatgctc aaaagcacaa atgcacccgt cctcaattca ctcgaagaac tgaaaagttt
       61 ggggttgtga agaaaaaaac gttgtttgac attattgctt caatatggta atatttatcg
      121 tttgttagca tggagagcca gttgagatca tccgaagaaa taaagaaaat tctaaatcaa
      181 aacatggagc acatgaaaaa cgagttttct gtcagtaaaa tcgggatatt cggttcttat
      241 gtcagagata gtgcccgtcc tgacagcgac atagacatca ttgttgaatt tgaagaaaaa
      301 tcgtttgata actatatgga tctgaaattt tttctggaag atctacttgg aaaaaaggtt
      361 gatcttgttg tttccgattc aataaaaaaa cgagctaaac cgacaatatt aaaagaagcc
      421 gaatatgtat agagatccaa tcctttattt ggaagacatt ttagacgcta taagaaaaat
      481 acgcgaatat attgcagaaa taacctttga agatttttgc gatgatgaaa aaaccgttga
      541 tgccgttatc agaaatcttg aggtgattgg tgaagccgca aagaatttgc cacaaaacat
      601 gaaagatatc catccggaaa ttgaatggcg caagatcaca gctataagaa atattgttgt
      661 ccaccaatat tttggggttg acaattttat catatgggat gtgatatcca ccaaacttgg
      721 ttctctttac gatgcagcga cagatctttt aaacaaaccc cagcaatttt cttaaaagct
      781 ttatctaacc gtattctcca cattcttttc aacctgatca agccatttct ctcttaattc
      841 atcagtgttt ttcaattata tctttgtcag aattttcgtc acttcaacat ccatcttgga
      901 aaagccgaac catttttttc caagatcttt gagtgagccg ccaaaatgat aaaggtcgcg
      961 attgtcaatt atcatgaatc tgtcatggga ataactgaat tcctttattt ctgctggtgg
     1021 gaactgttca ttgaatttct gcgtgtcgag ttgaagctgt tttgaaagat tttttgtgag
     1081 gattctgagt gtaactccct gtttccgttt tgaaaaaagt gtcagaactg tatcatcaac
     1141 ataattatca atgaggatga tcgacttttc agccgacctt acaatatctg aaacgaattt
     1201 gtgtgcttca aagacctgac cttcaaaaaa aatgccttgc ttgggaagaa gttgattcga
     1261 ttccagggct ttaaaaagat ccttgaattt actatctgct tctaattgtt taatttcaag
     1321 aaaatcaact cttttaaaaa ggtcggcttt acctgcaaga aaatgtctca tggctacgaa
     1381 agctctcatg attaaaacac ttgtctcaat agctttttca cttgtaataa cagaagaaac
     1441 ggaagccaca ccctgctctg taaacacata cggcaaaaaa ccaccaagat gtctttttga
     1501 aggtatcgca ttttgcgata ccataaaatc tgcttcaatt tcagtaagtt gaaacataaa
     1561 atcagatgga aatcttttga tattcctttt tacctgttct cttaatttga ttgttttgac
     1621 agaataaaat gttgcaatat ccctatccag catcacctgc attccacgga tagtaaaaat
     1681 tctgctctta atatcatcaa tctgtatcaa ctcattttct gccattttcc gactccaaaa
     1741 atttagtcac cgatagataa tgcaacaata gaagtaaatt tgcaagaaaa cagataagat
     1801 tatttaaggc gcggtttata ttttgttttc agaaaaacaa gctatttaac cattttcccc
     1861 acactttttt caacctgatc aagccatttc gctcttaatt catcagtgct ttttttcatt
     1921 ctgtcaaact ggacgatctt aacctttttg actcctataa ttttaagtgt tgtgtcggca
     1981 acgactttct tgaccgggaa aaagagcatg aggttgtgat aaattttggg tcctccgaga
     2041 gtgttgaaga taaccgcttg ttttatttga ggaaaaagcg gtttaagcat tcctgatttt
     2101 ttataaatat atgccctttt gggaagaaaa actttgtcaa agaacccttt taatattgca
     2161 ggcatcactc cccaccagac tggaaaaatg ataaaaatgg aatctgtttc agccagcatt
     2221 tcaagatatc tgtctttgaa aaacaacaca agtgctgaat gaatataact taaaccttca
     2281 gaaactgga
//
LOCUS       DAOOYB010000382         2271 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_59927_length_2271_cov_5.362816, whole genome shotgun sequence.
ACCESSION   DAOOYB010000382 DAOOYB010000000
VERSION     DAOOYB010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59927_length_2271_cov_5.362816"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..917
                     /locus_tag="PL056_13715"
     CDS             <1..917
                     /locus_tag="PL056_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HPM48119.1"
                     /translation="EAMDFRVSVCPVIFGEKVVLRLLDKSNLQLDMTKLGFYPDQLEI
                     FKDSIHKPYGMVLVTGPTGSGKTTTLYSALSELNQDDVNICTAEDPVEFNLEGINQVA
                     IREQVGLTFAAALRSFLRQDPDIIMVGEIRDFETGEIGVKAALTGHMVLSTIHTNDAP
                     STVTRLLNMGIESFLVTSSVNCIVAQRLGRQICKKCKEPIDVPKSELLKLGIKESQIA
                     GGYQIYQGKGCEICNGTGYKGRIAFYEVMKMSDPLKDAILNGASSMELKEIAMQHGMK
                     TMRISANLRLLEGVTTIAEVIRNTAPDE"
     gene            933..2030
                     /locus_tag="PL056_13720"
     CDS             933..2030
                     /locus_tag="PL056_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HPM48120.1"
                     /translation="MSDAPQVSLQDLLTLMIEQNASDLHITSGSPPQLRIDGSLRPVN
                     VPPLSPTETKRLAYSILTEKQKKQFEEKNELDLSFGVKNLSRFRANVFMQRGAISAAI
                     RVIPYKILDFKQLGLPPIVEELAKKPKGLILVTGPTGSGKSTTLASIVDYINRNRKGH
                     IVTIEDPIEYIHSHKNCIVNQREVGADTGSFAAALRVVLRQDPDVVLIGEIRDLETMA
                     TALSVSETGHLALATLHTNSTIETINRIIDMFPVNQQAQVRAQLSFVLVGIVAQTLVP
                     KKYGGGRTIACEIMVPNAAIRNLIREEKLQQIYSSMQSGQKDSNMQTMTQDLARLVKN
                     DIIDMNEALAIVPFVDEFQNLLAKSNQPTRR"
     gene            2033..>2271
                     /locus_tag="PL056_13725"
     CDS             2033..>2271
                     /locus_tag="PL056_13725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HPM48121.1"
                     /translation="MPFYRYSGVNKKGETVKGSIDADDEKGARQALMAKGVRPDSIKK
                     DWTRIELGGGGIGKKELVIFTRQFATMINAGLSII"
BASE COUNT          685 a          491 c          528 g          567 t
ORIGIN      
        1 aagaggcaat ggatttccga gtatctgtat gtcctgtcat attcggagag aaagttgttc
       61 tccgtcttct tgataaatca aatcttcagc ttgacatgac aaaactcgga ttttatcctg
      121 accagcttga aatattcaag gattccattc acaagccata cggaatggtt cttgttacag
      181 gtcccaccgg ttccggtaaa acaacaactc tttattcggc actttccgag cttaatcagg
      241 atgatgtcaa tatttgtacc gctgaagacc ctgtcgagtt caaccttgaa gggataaatc
      301 aggtcgcgat cagagaacag gtcggactca catttgccgc tgctctcaga tcctttcttc
      361 gtcaggaccc tgatatcata atggtcggtg agatccgaga ctttgaaaca ggcgagatcg
      421 gtgtaaaagc agctctcacc ggtcacatgg ttttgagtac gatacacact aacgatgccc
      481 cttcaacagt cacccggctt ttgaatatgg gtattgaaag ttttcttgtc acatcatcag
      541 taaactgcat tgttgctcag cgactcggga ggcagatctg caaaaaatgc aaggaaccca
      601 tcgatgtccc caaatcagaa ctcctgaagc tcgggattaa agaatcccag atagccggag
      661 gatatcagat atatcagggt aaaggttgtg aaatatgcaa cggtaccggc tacaaaggcc
      721 gaatcgcttt ctatgaagtt atgaaaatgt ctgatccttt aaaagatgcg atccttaacg
      781 gagcgagttc aatggaactt aaggaaattg caatgcaaca cggcatgaag acaatgagaa
      841 taagcgcaaa ccttagactt cttgaaggtg ttactacgat agctgaagta attagaaata
      901 ctgctcctga cgaatagata ttgaggtaaa aaatgagtga tgctccacaa gtttccctgc
      961 aggaccttct tacgctgatg atcgaacaga atgcatccga tctgcatatt acaagcggaa
     1021 gtccgccgca gctcaggatc gacggatctc tccgtccggt aaatgttcct cctctttctc
     1081 cgactgaaac aaagcggctt gcttacagca ttctgactga aaaacagaaa aaacagtttg
     1141 aagaaaagaa cgaacttgat ttgagtttcg gtgttaagaa tctgagtcgt ttcagggcga
     1201 atgtgtttat gcagagaggg gcgataagtg ccgcaatacg agttatccct tataaaatcc
     1261 ttgatttcaa acagcttggt ctgcctccca tagttgaaga acttgctaaa aaaccgaaag
     1321 ggctcattct tgtcactggt ccgacaggaa gcggaaaatc aactacgctt gcatctattg
     1381 tagactatat caacaggaac agaaagggac acattgtaac catcgaagat ccgatagaat
     1441 acattcacag tcacaagaac tgtatcgtca accagagaga ggttggagcc gacaccggtt
     1501 ctttcgccgc cgctctccgc gttgttcttc ggcaggaccc tgatgtggta ctgataggcg
     1561 agataagaga ccttgaaaca atggcgacgg cgctcagtgt ttctgaaaca ggtcaccttg
     1621 cacttgccac tcttcacaca aacagcacta tcgaaacgat aaaccgtatc atcgacatgt
     1681 ttcctgtaaa ccagcaggca caggtgcgtg ctcagctcag ctttgttctt gtggggattg
     1741 ttgcccagac acttgtcccc aaaaaatacg gaggggggag aacaatagcg tgtgagatca
     1801 tggttccgaa tgcggcaata agaaatctta tcagagaaga gaagcttcag cagatatact
     1861 caagcatgca gtcagggcag aaggacagta atatgcagac aatgacacag gatctggcac
     1921 ggcttgtgaa aaatgatata attgacatga atgaagctct ggcaattgtt ccttttgtag
     1981 atgaattcca gaatcttctg gcaaaatcaa atcagccgac aaggaggtaa ttatgccgtt
     2041 ttacaggtac tccggagtaa acaaaaaagg tgaaactgta aagggttcca ttgatgcaga
     2101 tgacgaaaaa ggtgctcgtc aggcattgat ggcaaaaggg gtccgtcctg attccataaa
     2161 gaaggactgg acccgtattg agctcggcgg cggcggaata ggtaaaaaag agcttgttat
     2221 tttcacccgt cagtttgcaa ctatgatcaa tgccggtctt tcgatcattc a
//
LOCUS       DAOOYB010000383         2253 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_60599_length_2253_cov_7.204732, whole genome shotgun sequence.
ACCESSION   DAOOYB010000383 DAOOYB010000000
VERSION     DAOOYB010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60599_length_2253_cov_7.204732"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            92..1369
                     /locus_tag="PL056_13730"
     CDS             92..1369
                     /locus_tag="PL056_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HPM48122.1"
                     /translation="MSDYNQMWKDLGLDIPNHEGLLNFLGEAYQGIYLSQQNRPKSMD
                     YFDFVISEIHGLRVKEIIDARKVGKKGIGTFCVYVPEELVLAAGGIYIGLCAGAEVGT
                     PQAEQYLPRNTCALIKAFVGFKLANLCPYGEVTDLIVGETTCDGKKKAYEAFDEITGK
                     MHIMDLPNTKSEEGKKLWLAEVKKLKDKLEAVTGNKITAENLAEATKIVNEKRRALQR
                     LAKLREANPSPISGLDALLINQISFYDDPLRFTSMVNKLCDELEERVNEGTGVCAENA
                     PRILVSGSPMAIPNWKLHHIIETSGAVVVGEESCVGARNYRALTDESAASLDDAVEKI
                     ASRYLTIDCACFTPNIERMENIEKMKDELKADGIVHYSLQFCTLYMTEGFKAANKMKD
                     FPFLRIETDYNMEDAGQIKTRIEAFLEMINTGK"
     gene            1391..2134
                     /locus_tag="PL056_13735"
     CDS             1391..2134
                     /locus_tag="PL056_13735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HPM48123.1"
                     /translation="MLSTGIDIGSSNIKIAVIENGLIIDLKKTSTGFNPVEKCRELIG
                     DNINGTVKATGYGRHLAEKAFNCGTVSEISAFAKGAVFFNPEVRTVIDVGGQDSKVIR
                     CNRSGEVLSFEMNDKCAAGTGKFLEIMAHALEMTQTEFGNIKPSNSKLIKISSICTVF
                     AESEVISMVGKGESKENIGYALHEMAVERVCSLTNRVGVDGIVSFAGGCAYDRLFVQL
                     LAKKLNRDIFVSAHPEFTGAVGAALLSAG"
BASE COUNT          720 a          424 c          538 g          571 t
ORIGIN      
        1 ttctcagccg gttttaccaa gcaggttcga ttcctgccct cgattgatgt tttgatttga
       61 tttaaaaatt ttaaaaatta cggagtgaaa aatgtctgat tacaatcaaa tgtggaaaga
      121 tctcggtctt gatattccga atcacgaagg gttgctcaat tttcttggag aagcttatca
      181 ggggatttat ctttctcagc agaatcgtcc taagtctatg gattatttcg attttgtcat
      241 ttcagagata cacgggctca gagttaaaga gataattgac gcaagaaaag tgggcaaaaa
      301 gggaataggg actttctgtg tctatgttcc tgaagaactt gtccttgccg caggcggaat
      361 ctacatcggt ctttgtgccg gagctgaagt gggaactcct caggcggagc agtatcttcc
      421 aagaaacaca tgcgctctta tcaaagcgtt tgtcggtttt aaacttgcaa atctctgccc
      481 atacggcgaa gtgaccgatc tgatagtcgg cgaaacgact tgtgacggaa agaagaaagc
      541 gtatgaggca tttgatgaga tcactgggaa aatgcacata atggacctcc cgaacacaaa
      601 atctgaggaa gggaaaaagt tgtggcttgc agaagtgaag aagttgaaag ataaactcga
      661 agcagttaca ggaaataaga taactgctga aaatcttgct gaagcgacaa aaatagtaaa
      721 cgaaaaaaga agagccctgc aacgcctcgc aaaattaaga gaagccaatc cttcgcctat
      781 ctcaggactt gacgcactgc tcatcaacca gatctctttt tatgatgatc cgctgagatt
      841 cacatcaatg gtaaataaac tttgtgacga acttgaagaa agggtgaatg aaggtactgg
      901 tgtttgtgcg gaaaatgctc ccagaatcct tgtttcgggt tctccgatgg caataccgaa
      961 ctggaagctt catcacataa ttgaaacttc aggagcagtt gtcgttggtg aagaatcatg
     1021 tgtaggtgca aggaattacc gtgctctaac cgatgaatcg gcagcttcac ttgatgatgc
     1081 agttgaaaag atcgcaagcc ggtatctcac aattgactgt gcatgtttta ctccaaacat
     1141 tgaaagaatg gaaaatatcg aaaaaatgaa agatgaactc aaagctgacg gcattgttca
     1201 ctattctctt cagttctgta ctctctacat gacagaaggt tttaaggcgg caaataaaat
     1261 gaaagatttt ccgttcctgc ggatcgagac cgactacaac atggaagatg ccggtcagat
     1321 caagacaagg atagaagcgt tcctcgaaat gataaacaca ggaaaatagc caaaataaaa
     1381 ggagaatcac atgctttcaa cggggattga catcggttcg agcaatatca agatcgcagt
     1441 cattgaaaac ggactgatca ttgatcttaa aaaaacttca accggtttca accccgttga
     1501 gaagtgccgg gaacttatcg gtgacaatat caatggcaca gtaaaagcga caggttacgg
     1561 aagacacctt gctgagaaag ctttcaactg cggcacggtg agtgaaatat cggcatttgc
     1621 aaaaggagcc gtttttttta atcctgaagt gagaacggtt attgatgtag gcgggcagga
     1681 cagcaaagtg ataagatgca acagatctgg agaagtgctc tcttttgaaa tgaacgataa
     1741 atgtgcggca gggacaggca agtttctcga gataatggct catgctctcg aaatgacaca
     1801 aactgagttt ggaaacataa aaccgtcaaa cagcaaattg ataaagatca gttcaatatg
     1861 cacagttttt gcagaatctg aagttatatc aatggtcgga aaaggtgaat caaaggaaaa
     1921 catcggttac gcacttcacg aaatggcggt ggaacgggta tgttctctaa ccaacagggt
     1981 cggagttgac ggaatagtct cttttgccgg cggatgtgcc tatgaccgtc tttttgttca
     2041 gttacttgcc aaaaaattaa atagagatat ttttgtgagt gcacatcctg aatttacagg
     2101 ggcggtcggt gccgcactgc tgagtgcagg gtagtcagat ttaatcagga gaacccatgg
     2161 aaaaaagatc aatgcagtca gaactcagaa agcttccctc agttgattct gttttaaacc
     2221 agccggtcat cagaaagcag atcgagcgca atg
//
LOCUS       DAOOYB010000384         2239 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_61127_length_2239_cov_12.317308, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000384 DAOOYB010000000
VERSION     DAOOYB010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61127_length_2239_cov_12.317308"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(150..1355)
                     /locus_tag="PL056_13740"
     CDS             complement(150..1355)
                     /locus_tag="PL056_13740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48124.1"
                     /translation="MTKREIVLRKAFVFSTYFAQGLPYSLIAVIPTVMFRDLGADLST
                     IGFLSLLNIPWVIKFLWAPFVDSNSTFRKWIYSTELLLTLAVSMIALSLFSEGLAIVT
                     VLLLLGAVMSATHDIAVDGYYMTSLDEISQKKEIGFRVLAYRLALAFGSGVIISIGTI
                     FSWRCAFGTAAFSMLMIYLIHKFFLPEPLNTKPSTPNSFLNFKKAFSTFFKRENIVHI
                     VMFIVFLRAGEYLLGLMRGPFMVDLGIKVHIGWISGGVAIPATILGAISGGYIISKKG
                     IDLTAVPIILFQNLTNLLYAALAFHFADKIITDNFGIAAVALVNAIESFSSGMGTALL
                     MIFLINLCAKEFKAAHYAIGSGLMALSGALLNSFGGIVAESTGYGWFFVISFLAAMPA
                     LLFFRKELM"
     gene            1510..2166
                     /locus_tag="PL056_13745"
     CDS             1510..2166
                     /locus_tag="PL056_13745"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPM48125.1"
                     /translation="MFSCADPYGIYHKVKKGETLYRISRIYDTDVDKLKKTNNITDET
                     KLKVGDYVFVPGVKAPAMWDGTSADDHSNVKKEDSGKTIPSEKVAKKSAPSKFIWPAQ
                     GVLTSTFGNRWGTKHEGIDIGCPEGTPVYASAAGKVIFSGTRGGYGLVIIIQHPDDWF
                     TIYAHNSKNLAAQGADVKQGDKIALSGKTGRATGPHLHFEIRQGVKPLDPIEFLPKVP
                     "
     gene            2157..2233
                     /locus_tag="PL056_13750"
     tRNA            2157..2233
                     /locus_tag="PL056_13750"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2191..2193,aa:Arg,seq:cct)
BASE COUNT          709 a          466 c          500 g          564 t
ORIGIN      
        1 ttaggatctt cgtctccgca tgaaaccata aaaaaagaaa acattaaaac tgccgtgatc
       61 gttaaaaaat ttctcatatt gctacctccg gttttgacac aactgccaaa tattaaatcg
      121 tgttcaaatt attgtcaaga aaggagagat cacataagtt cctttctgaa aaaaagaagt
      181 gcaggcattg cggcaaggaa cgaaatgaca aaaaaccagc cgtatccggt gctttcggca
      241 acgattcctc cgaaactgtt gaggagtgct ccgcttagtg ccataagtcc tgatccgatg
      301 gcgtaatgag ccgctttgaa ctcttttgca cagagattta tcagaaatat catcaaaagt
      361 gcggttccca tgccgcttga gaaactttcg attgcgttta caagggcgac tgcggcaatg
      421 ccgaaattat ctgtgatgat tttatcagca aaatgaaaag caagtgcggc ataaagcagg
      481 tttgtgagat tctgaaaaag gattatcggg acggcagtta gatcaattcc tttttttgag
      541 atgatgtaac cgcctgatat tgctccaaga attgttgccg ggatggcaac gccgcctgat
      601 atccagccga tgtggacttt tattccaaga tcaaccatga acgggcctct catcagcccg
      661 agaagatatt cgccagcccg taagaacaca atgaacatca caatatgaac aatgttttcc
      721 cttttaaaaa atgttgagaa tgctttttta aaattcagaa aactgtttgg agttgagggt
      781 ttagtgttta gcggttcagg gagaaagaac ttgtggatca gatagatcat gagcatggaa
      841 aaagcggcag ttccgaatgc acagcgccac gaaaagattg ttccgatcga aattatcacc
      901 cctgatccga atgcaagtgc aaggcggtat gcaaggactc tgaaaccaat ctcttttttc
      961 tgggaaattt catcaaggga agtcatgtag tatccgtcaa ctgcaatatc gtgagttgca
     1021 gacatgaccg ctccgagcag aagaagcacg gtgacaatgg caagtccttc agaaaagagc
     1081 gacaatgcta tcattgaaac tgcaagagtc agaagaagtt cagttgaata tatccatttt
     1141 ctgaaagttg aatttgaatc aacaaaaggg gcccacagga attttatcac ccacggaatg
     1201 ttcaaaagcg aaagaaagcc gatcgttgac aagtcggcac cgagatctct gaacatgaca
     1261 gtcgggatca ccgcaatgag agaatatggg agaccctgag caaaatatgt tgaaaaaaca
     1321 aaagcttttc taagcacaat ttctcttttt gtcatcagat ctctcttcaa agaacattat
     1381 aataataggt tttagtgatt gttttgcaat gttgttgaca gacattttct taaaaactat
     1441 aatcagcaga atcttttttg aggtgtcgag attaaaaagt ttctttttct cctacttatc
     1501 atattcctga tgttctcatg cgcagatcct tacggcattt atcacaaggt caaaaaaggg
     1561 gagacgcttt atcgcatatc gaggatatat gataccgatg ttgacaaatt gaagaaaaca
     1621 aataacatca ccgacgaaac aaagctgaag gtcggcgatt atgttttcgt tccaggggta
     1681 aaggccccgg ctatgtggga cggcacttca gcagatgatc attcaaatgt gaaaaaagag
     1741 gattcaggca agaccattcc ttctgaaaaa gttgcaaaga aaagcgcgcc gtcaaagttc
     1801 atctggccgg cacaaggcgt cttgacatca acattcggca acagatgggg cacaaaacac
     1861 gaaggaattg acatcggatg tccggaagga acacccgttt atgcctccgc cgcagggaaa
     1921 gtgatctttt caggcactag aggaggatac ggacttgtta taattattca gcatccagac
     1981 gactggttca ccatttatgc ccataattcc aagaacctcg ccgctcaagg tgcagatgtg
     2041 aagcagggag acaagatcgc actctcagga aagaccggaa gagcgacagg cccgcacctt
     2101 cattttgaaa taagacaagg cgttaaaccc ctggatccca tagaattttt acccaaggtc
     2161 ccgtagctca gacggataga gcgatcgcct cctaagtgat aggcaacagg ttcgactcct
     2221 gtcgggacca ccatttttt
//
LOCUS       DAOOYB010000385         2231 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_61469_length_2231_cov_5.501838, whole genome shotgun sequence.
ACCESSION   DAOOYB010000385 DAOOYB010000000
VERSION     DAOOYB010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61469_length_2231_cov_5.501838"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="PL056_13755"
     CDS             <1..263
                     /locus_tag="PL056_13755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HPM48126.1"
                     /translation="VMGAKGAIEILMKKELDAAEDKQKFMAEQEELYNENVANPYIAA
                     QHGYIDDIFEPKTTRPRLIKALEMLSSKNDDLPAKKHGNIPL"
     gene            276..713
                     /locus_tag="PL056_13760"
     CDS             276..713
                     /locus_tag="PL056_13760"
                     /inference="COORDINATES: protein motif:HMM:NF016187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family transporter subunit"
                     /protein_id="HPM48127.1"
                     /translation="MNSINFLQNAVGAFEGLSRSDQLIVKTLPFLGISVVIGGLSILA
                     IILTQFKRLGEGKAHGHGNAAKPAEKKKTEEPVADKPAQNVSDDSEMDQYVAAIALSL
                     YISENSEKVYLTEVNQFHNQNPWTSSGISGRASRFKKWQSLKK"
     gene            724..1161
                     /locus_tag="PL056_13765"
     CDS             724..1161
                     /locus_tag="PL056_13765"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HPM48128.1"
                     /translation="MKKYEMKINGKDYSVEIKDFNSKGAKVTVNGKTYDVGVKYTETE
                     PPVIPVISRKAAAPLEKQPAQPAVSETSSGSVSSVFAPMPGLILKILVKPGDSVSIGQ
                     KVVSMEAMKMENDINSTVAGTIKEVLVKEGDNVREHQSLLTIG"
     gene            1763..>2231
                     /locus_tag="PL056_13770"
     CDS             1763..>2231
                     /locus_tag="PL056_13770"
                     /EC_number="7.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HPM48129.1"
                     /translation="MTKFFNTTAFAHITLGNVIMMLVAFIFIFLAIAKDYEPLLLLPI
                     GFGILIGNIPTAAGVQIGVYDPGSVLNYLYFGVLKGIYPPLIFLGIGAMTDFSYMIAN
                     PKLVLLGGGAQLGIFIAFLIAMLLGFSIQEAASIGIIGGADGPTAIFVTGLMSP"
BASE COUNT          694 a          472 c          493 g          572 t
ORIGIN      
        1 ctgtcatggg cgcaaaaggt gctatcgaaa tacttatgaa aaaagaactt gatgcagcgg
       61 aagacaagca gaaattcatg gctgaacagg aagaacttta caacgaaaat gttgccaatc
      121 cctacatcgc agctcagcat ggatacattg atgacatttt tgaaccgaag acgacaagac
      181 cgagacttat aaaagctctc gaaatgcttt catcaaaaaa tgacgatctg ccggcgaaga
      241 aacacggcaa cataccgctc taaggaggga tcatgatgaa ttctatcaac ttcctgcaga
      301 atgcggtggg tgcttttgaa ggtctttcga gatcggacca gcttattgta aagacacttc
      361 catttctggg aatatctgtt gtaatcggcg gactttcaat tctggcgata atccttaccc
      421 agttcaaaag acttggtgaa ggtaaggctc acggacacgg aaatgccgca aaacctgcag
      481 aaaaaaagaa aacggaagaa ccagttgcag ataaaccggc tcagaatgtt tctgacgatt
      541 ccgaaatgga tcagtatgta gccgcaatag ccctttctct ttacatatcc gaaaattcag
      601 agaaggtcta tcttaccgaa gtaaatcagt tccataatca gaatccgtgg acatcatcag
      661 gaatatccgg cagggcatcc cgctttaaaa aatggcagtc attgaagaaa tgaggagaac
      721 gaaatgaaaa agtatgaaat gaaaataaac ggtaaagact acagtgtcga aataaaggat
      781 ttcaactcaa agggagccaa agtgaccgtg aacgggaaaa catacgatgt cggtgtgaaa
      841 tatactgaaa cagagccccc cgtcattccg gtaatttcga gaaaagcggc cgctccttta
      901 gaaaaacagc cggcacagcc tgcggtttct gaaacatcat caggcagtgt cagcagtgtt
      961 tttgcaccga tgccgggact tatcctgaaa atccttgtaa aaccgggtga ctcagtttcc
     1021 atcggtcaga aagtggtatc aatggaagca atgaaaatgg aaaacgacat taacagcaca
     1081 gttgccggaa ctataaaaga ggtcttggta aaggaagggg ataatgtccg cgaacaccag
     1141 tcgcttttaa ccataggata gaacagaggg aaaaatgaat attttaaaaa gatccgtact
     1201 gctgctgaca attctccttt caggttttgt cattcacgca caagacggtg catctgaaaa
     1261 ctttacgggg gtttttgaaa acaaagctct tgtgaaactg agcgtaagat attttgttgc
     1321 cggtcaatgg aaagaaagaa ctgagttcgg tgcaatcgtg tctgacaatt attcaactga
     1381 aaaagacaag acagagccgg gaagctatct tgtaataaca ggccagagcg ctgttcttcc
     1441 cgattccgac gaaattgatc tgtttatacc ggcagacaac aactatgagg ttaccgcaac
     1501 tttttcagac agcgtacaga aaaccttcag gcatcaggac atcatcgttg ccgataacgg
     1561 ggtttcgttt atcaatttca aatcggaccc gggcagatat ccggtgatga tctcgggtga
     1621 aagtgtcgga gatgttgtca taaacaactc ggtttatatt aagaatttta aaagcagtta
     1681 ccagataact ccccttttca tcgacttttc gctcagatcg tcaagtaccc ttcctgaata
     1741 tgttcaaaag aacaaaagtt tcataacaaa gttcttcaac acaactgctt ttgcacatat
     1801 tactctcgga aatgtcatta tgatgcttgt tgcgttcata ttcatttttc ttgcaatagc
     1861 aaaggattat gagccgcttc ttctccttcc gatcggtttt ggaatactta tcggcaacat
     1921 ccccactgcg gcaggagttc agatcggtgt atatgatccg ggatcagtcc tcaactacct
     1981 atatttcggg gttctcaaag gtatttatcc ccctttgata ttcctcggaa taggtgcgat
     2041 gactgacttt tcatacatga ttgccaaccc gaagcttgtc cttcttggcg gaggagctca
     2101 gcttggaata ttcatagctt ttcttattgc aatgcttctg ggtttctcaa ttcaggaagc
     2161 ggcatcaata ggaatcatcg gcggagctga cggtccgaca gccatatttg taaccggact
     2221 catgtcaccc c
//
LOCUS       DAOOYB010000386         2208 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_62403_length_2208_cov_10.689271, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000386 DAOOYB010000000
VERSION     DAOOYB010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62403_length_2208_cov_10.689271"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(174..1532)
                     /locus_tag="PL056_13775"
     CDS             complement(174..1532)
                     /locus_tag="PL056_13775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48130.1"
                     /translation="MISYLFYTILVIVACSCNSPEKSNDFDLDLSDFDSEKMTDFSEE
                     EESCLNDEEYDSWDYVLDLGQVDHVSRIIPYKKGYLLSGVIDFPYKPNSDLYLPGYVA
                     FMNKDYSFGWQVLIDCFLIGSVATDGVGKIFLGGDIPNSYSMALASIDENGNVLWKKN
                     WSDDSFFKSGPQGISKVKYFDKSLYLSGFSGGWNEDGSMTFHKLYLAKTDLAGNILWK
                     KVWGAEGHNYFNDMMIDEKGFMYLTGNTNGDFEGNENANEMGDCGDERHACGDVILVK
                     TDMEGNVIWARQWGSDKKTEYEYGQQIILDEDQNIFVLSLIYSSDLVSSEVRKFDSDG
                     NLIWSKIFKSEQPETYFYPQKTLIDKDGSFKFAAVEMILESRKTTIFIINLDRDDGEV
                     IDSISIPSENEIEIYGLESLDDKLILMGQYYFEYNQTGYSDTFFKEINRDCFKKAEKM
                     DL"
     gene            complement(1763..2047)
                     /locus_tag="PL056_13780"
                     /pseudo
     CDS             complement(1763..2047)
                     /locus_tag="PL056_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013164322.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
BASE COUNT          693 a          449 c          310 g          756 t
ORIGIN      
        1 aacagcgact aagtttcatg tcaaattatt tttggttcgt tttgtattca aacttatata
       61 tcctaattta aacataatga tttctgatgt ttgtgaacgc ttcaggatct taggaaagct
      121 gataacgact gtgagttatt gaaaagatta atctttcgct tcttctgtca ccgttacaaa
      181 tccatctttt ccgccttttt aaaacagtct ctatttattt ctttaaaaaa tgtatcagaa
      241 taaccagttt gattatattc aaaataatac tgacccatta aaatcagctt gtcatcaagt
      301 gattccaatc cataaatttc aatttcattt tcactgggaa tggatattga atcaatcact
      361 tctccatcat ccctgtccag atttataata aaaattgttg ttttcctgct ttccagaatc
      421 atttcaacag ctgcaaattt aaaacttccg tctttatcaa ttaaggtttt ttgtggataa
      481 aaataggtct caggttgttc tgatttaaat atttttgacc agatcagatt accgtcagaa
      541 tcaaattttc tcacttctga tgacaccaga tcggatgaat aaataaggct taaaacaaaa
      601 atattctgat cttcatccag aattatctgt tgtccatatt catattctgt tttcttatca
      661 cttccccact gtcttgccca tatgacattt ccttccatgt cagttttaac aagaattaca
      721 tctccacagg catgtctttc atctccgcag tcacccattt catttgcatt ttcattccct
      781 tcaaaatcac catttgtatt gccggttaaa tacatgaatc ctttttcatc aatcatcata
      841 tcattgaaat aattgtgacc ttccgctccc catacttttt tccataaaat atttcctgca
      901 agatctgttt ttgccaaata aagcttatga aatgtcatcg agccatcttc attccagcca
      961 cccgaaaatc ctgataaata caaagacttg tcaaaatatt ttactttgct tattccttga
     1021 ggtcctgatt taaagaaact gtcatcactc cagttctttt tccataaaac atttccgttt
     1081 tcatcaattg aagcgagagc catggaataa gagttcggaa tatctcctcc taagaaaatt
     1141 ttcccgacac catcggttgc aacacttccg attaaaaaac aatcgattaa aacctgccag
     1201 ccaaacgaat aatctttgtt cataaatgcg acgtatccgg gaagatacag atcgctattc
     1261 ggtttataag gaaaatcgat aacaccggaa agaagatatc cctttttata gggaataatt
     1321 ctgcttacat gatcaacctg accaagatca agaacataat cccacgaatc atattcttca
     1381 tcatttaaac acgattcctc ttcttctgaa aaatccgtca ttttctcgct atcaaagtca
     1441 gaaagatcta aatcaaagtc atttgatttt tcaggagagt tgcaggaaca agcaacgatc
     1501 actaaaattg tgtaaaacaa ataagatatc aaatattcct cctacaaaaa aagcactgtc
     1561 cagccacaag actggacagc agcgtttttc aataattata agggtaacct aaatatgtac
     1621 aatagctttg tagttgcgag aaagaacatc cattccacac aggattaaag tactgtgctc
     1681 cggaagaata tgtggatacc cagttcggag aaaaaattct gatttgagca gatgtgagtt
     1741 ctttcggaat caggttcttt tttattgcat ttgtggcatt tatccatttt tgaggagaca
     1801 tcgtaaacgc attcaatcct gcttcttttc taaacccctc gaattcgagg ggtttaaaat
     1861 actgattgta aagggtttcc caaataccta gaaattctat tgtgttacga tttctcatcc
     1921 aattaccgat cacagcactt ggatcatctg ttttgtattt tgcaatatcc gttagcgata
     1981 tatagtccaa atcatttacg gtgacaattg caacttctgt ctctttcaca ttgatctttc
     2041 tgttcatgtg tgcctcactt taattttaga catatctaac atgtcattcc aaattttata
     2101 tcatagactt taaaaataaa ccattttttt atttatcaga aagaatttcc cagagaaaaa
     2161 tagaaattga gttgtccgct ttttgtcaat ccgtaggcgg ttccgaat
//
LOCUS       DAOOYB010000387         2185 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_63408_length_2185_cov_2.756808, whole genome shotgun sequence.
ACCESSION   DAOOYB010000387 DAOOYB010000000
VERSION     DAOOYB010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63408_length_2185_cov_2.756808"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1421
                     /locus_tag="PL056_13785"
     CDS             <1..1421
                     /locus_tag="PL056_13785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48131.1"
                     /translation="ALYKMIAPANFSTVEGELQYNGSFPEIFTCNETEVDEENQNCDE
                     IFGKTGQDLWRDLIKNIDVSEIKICISTGNDGSDDDVYVKFNDVDDKVAGNNDGYRPS
                     LFYLDNAKNNFEPNVQECFIIDKEQVQVFEIGDIKFIKLAKYPFYRDGDRPDLNPGTD
                     GVDIKEVSLFVNGVNIFKKVFSPDFFMDGNDGGVPELVITKEEIFSSELCRLENSKRM
                     AVFSGEVFNEYPIVDFVSEITVESLKARIEDSVGHKTEVENTYHQETDYKPIDLHLET
                     STDRVKLNYVDTHKIEVSFGLTDSENYLSDWDMYVFADIEITRFEDVDENGNTVFKRR
                     IEMKNFTYDFDGHEALMNLLTAFVTVMSGYTWFTSIEFFVADHILWSSEVPYGVNFED
                     YIEQQKPMDLEEGISEGIITDIYFTEDYTLHFEHSDLVRGLKFFNNVFLTCIDDEIEG
                     SLDCLQRYPNTIELIENLTSGE"
     gene            1535..1999
                     /locus_tag="PL056_13790"
     CDS             1535..1999
                     /locus_tag="PL056_13790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48132.1"
                     /translation="MEYYYEVPSGVLYVNNIKSFCESLDFEGGGWYWATINDLRNLVT
                     GCPKLEPGGICKVANDCRSEEYCYSRGNCDCEVEETEKSDDDTLKTISLLHRSDSEKS
                     LSVIYSSTCNTCYEGADYIEYWTIYFSLSGHVSPDYSTQPPSSDIICVRKKE"
BASE COUNT          742 a          320 c          452 g          671 t
ORIGIN      
        1 ttgcattata taaaatgatt gctcctgcaa atttttcaac tgttgagggt gaattgcagt
       61 ataatggatc ttttcccgaa atatttacct gtaatgaaac agaagttgat gaagaaaatc
      121 agaattgtga cgaaattttt ggcaaaacgg gtcaggattt atggcgggat cttataaaaa
      181 acatagatgt aagcgaaata aaaatctgta tttcaacagg caacgacggt tcagatgatg
      241 atgtgtatgt taaatttaat gatgttgatg acaaagttgc gggaaacaat gatggataca
      301 gaccatcact attctacctt gataacgcaa aaaacaattt tgaaccgaat gtccaggagt
      361 gttttataat tgacaaggaa caggtacaag tatttgaaat tggtgatatt aaatttataa
      421 agcttgcaaa gtatcctttt tacagagacg gtgacagacc tgatcttaat ccaggaactg
      481 acggagttga cataaaagag gttagcttat ttgttaatgg tgtaaatatt tttaaaaagg
      541 ttttctcacc tgattttttt atggacggaa acgacggggg agtgccggaa ttagttataa
      601 ctaaagaaga aatattttca agcgagcttt gcagactgga aaattcaaaa agaatggctg
      661 tgttcagcgg agaagttttc aatgagtatc cgatagtcga ttttgtcagt gaaataacag
      721 ttgaatcact taaagcaaga attgaagatt ctgtaggtca caaaacggaa gttgaaaata
      781 catatcatca ggaaacagat tataaaccga ttgatctgca tcttgaaaca agcacagata
      841 gagtcaaact caattatgta gatactcaca aaattgaagt ttctttcggt ctcactgata
      901 gtgagaatta tctttcagac tgggacatgt atgtttttgc agacattgaa attacaaggt
      961 ttgaggatgt agatgaaaat ggaaatactg tttttaaaag aagaattgaa atgaagaatt
     1021 ttacttatga ttttgacgga cacgaagcac ttatgaatct tttaactgca tttgttactg
     1081 ttatgagcgg atatacatgg tttacatcga tagagttttt tgttgcagat catattctct
     1141 ggtcaagtga agtcccttac ggagttaatt tcgaagacta tattgaacaa cagaaaccca
     1201 tggatcttga agaaggaatt tcagaaggaa ttattacaga catatatttt actgaggatt
     1261 atacgcttca ttttgaacat tcggatttgg ttagaggact taaattcttc aacaatgtct
     1321 tccttacttg tatcgacgat gaaattgagg ggtctctgga ctgtcttcag agatatccta
     1381 acaccattga actaatagaa aatctcacat caggagaata aacatgaata aaactatatg
     1441 tttgttttta cttttgatct tgtcaggatg caatgtcatt gacaatacag aacatcacgg
     1501 tgaacctcgg atcttcacac atccggaaac tggaatggaa tattattacg aggttccgag
     1561 tggagttctt tatgttaata atattaaatc tttttgtgaa agtctggatt ttgaaggtgg
     1621 tggctggtac tgggcgacaa ttaatgatct tcgcaatctt gtcactggtt gtccgaaatt
     1681 ggaaccgggc ggaatttgta aggtagccaa tgattgccgc agtgaagaat attgctattc
     1741 ccgtggaaat tgtgattgtg aagttgaaga gacggaaaaa agtgatgatg atacgcttaa
     1801 aacgatttct cttttacatc gatcagacag tgaaaagtct ctttctgtaa tttattccag
     1861 cacctgcaac acatgctatg aaggagccga ttatatcgaa tactggacaa tttatttttc
     1921 tttgagtgga catgtcagcc cagactattc tacacagcca ccaagtagcg acataatttg
     1981 tgtaaggaaa aaagaatgaa aagaaccgcc ctgtgttaaa ataatatctg cctaatgcca
     2041 agaaaatcac atccagtgcg attactgtca aaacgattac tgatcaaaac gattacttga
     2101 ataaaaatct gttagtgata aaattaatgt aataaatttc tctgtcggag gttgatttgc
     2161 tgtacatcgg gaaaaaaagg gagat
//
LOCUS       DAOOYB010000388         2181 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_63546_length_2181_cov_9.332079, whole genome shotgun sequence.
ACCESSION   DAOOYB010000388 DAOOYB010000000
VERSION     DAOOYB010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63546_length_2181_cov_9.332079"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..1929)
                     /locus_tag="PL056_13795"
     CDS             complement(100..1929)
                     /locus_tag="PL056_13795"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPM48133.1"
                     /translation="MKKIIACFALFILFFAIESAEIQPRLTGRIFIKLTDKCLKTGRN
                     SCLKSLGCVDAGKLTLKNWALAKCSGDPVEKSEEFVKKGLISRPERFVKTELYSIDPL
                     YLNDTYAQEQWNFHNTGNVTSADGKLSVKGNDHAHIFEAWQLLKTLGVVTDSEIGKER
                     KIGIIDDGFDILHDDLKDNFISWKNFSGPVEPGNMFSETAGTVANFHGTMVAGVAAAR
                     GNNLTGHSGACPACSIIAARMDADPTIDPGLTAEEYFDSIFNWVYEQGAEIINCSWGP
                     DADINAEYFGELFSYFATNGRGGLGTLIVFASGNSGEDFSWNPFATHPDTLSVGATDS
                     TGSRYDFSNYGTMLDIMAPAAGGEKSGTTKYFDRIWTTDNFLRPACLAEGAVPTNGCS
                     DQAGWTPNHPMAGGDGWWGKYSYRFSHTSSAAPLVTGVAALLLEANQALTAKEIKEIL
                     INTADKVSPMDANYDSNGHSNKYGFGRINALRAVAAAWIFGGGTITAGLKDDIDESSP
                     CTKEDCWNFEGAEYPDVETENDLNNQPDEDEDTEITDDTSFPDEDNGNNENNDADALT
                     VPDNGSKENSETENVPDEKSDDEQNVPDGSIEEEKAGCSVVRI"
BASE COUNT          581 a          497 c          411 g          692 t
ORIGIN      
        1 aagatgccgc tgaacttgtt gcaagaatat atcccaaact cggaaaaaat gagatcaaga
       61 agatctttat tgaatcaaaa cagaaatgaa ttcagccatt taaatcctga ccaccgaaca
      121 tccggctttt tcttcttcaa tcgatccatc aggaacattc tgctcatcgt cgcttttttc
      181 atccggaaca ttttcagttt cgctgttctc tttgcttccg ttatcaggaa cagtgagtgc
      241 atcagcatca ttgttttcat tgtttccgtt atcttcatca gggaaagaag tatcatcagt
      301 gatttcagta tcttcatctt catccggttg attatttaag tcgttttcag tttcaacatc
      361 cggatattcg gctccttcaa agttccagca gtcttctttt gtgcatggcg atgattcatc
      421 aatgtcgtct tttagtccag ctgtaattgt tcccccgcca aatatccatg ccgcggcaac
      481 tgctctcaaa gcatttatcc tgccgaaacc gtatttatta ctgtgtccgt ttgagtcata
      541 atttgcgtcc attggagaca ctttgtcggc ggtgtttata agtatctctt tgatttcttt
      601 tgcagtaagt gcttgatttg cttcaagtaa aagtgcggca acacctgtaa caaggggtgc
      661 tgccgagctt gtgtgcgaaa aacggtatga atattttccc caccagccgt cgcctcctgc
      721 catcgggtga ttcggagtcc agccggcctg atcactgcat ccatttgtcg gaactgctcc
      781 ttccgccaga catgcgggtc tcaaaaaatt gtcggtagtc catattctgt caaaatattt
      841 agtcgttccc gatttttctc caccagcggc aggcgccatt atatcaagca ttgttccata
      901 gttagagaaa tcatatcttg atccggtgct gtcagttgct ccgacagaaa gtgtgtctgg
      961 atgggttgca aaagggttcc acgaaaaatc ttctccgctg tttcctgatg caaaaactat
     1021 cagagttcca agtccaccgc ggccgttagt tgcaaaataa ctgaaaagtt cgccaaaata
     1081 ttctgcattg atatcagcat caggtcccca cgaacagtta ataatttcag caccttgctc
     1141 ataaacccag ttaaatatcg aatcgaaata ttcttctgcg gtaagtcccg gatcaattgt
     1201 gggatcggca tccatccggg cggcgataat tgaacatgcc ggacaagctc cggaatgacc
     1261 tgtcaaattg tttcctcttg ccgcagcaac tccggcaacc attgttccat gaaaatttgc
     1321 aacagttccg gcagtttcgc tgaacatgtt tcccggctca accggccctg aaaaattctt
     1381 ccagcttatg aaattgtctt taagatcatc atgcagaata tcgaatccat cgtctatgat
     1441 cccgattttc ctctcttttc cgatctcact gtctgtcacg acaccaagtg ttttcagcaa
     1501 ttgccatgct tcaaaaatat gtgcgtgatc atttcctttt actgaaagtt ttccatcagc
     1561 agaagtgaca tttccggtat tatgaaaatt ccattgttcc tgtgcgtaag tatcattcag
     1621 atagagcgga tctattgaat aaagctctgt ttttacgaat ctttccggtc ttgaaatcaa
     1681 acctttcttc acaaattctt ctgatttttc aaccggatcg cctgagcatt tagcaagtgc
     1741 ccagttttta agtgtcaatt ttccggcatc gacacatccg agacttttca aacatgaatt
     1801 cctacctgtt ttcaaacact tgtctgtaag tttaataaaa atacggcctg tcaggcgggg
     1861 ttggatctcc gcactttcaa ttgcaaaaaa aaggatgaac aatgcgaagc aggcaatgat
     1921 ctttttcata attatctccc gaaattaaca atcagtcaga caatatttaa agagtttcgg
     1981 ataattttcg acctctttcg caaagaaatt cctaaaaaaa gtccagatgc gtaaatttcc
     2041 attaaaaatg atatttgctt ttaactttat ttgataatat atttttctgc gatctttaag
     2101 gagagatgat gtcaatcgga tttacaataa tttttctgat cggtgctgtg cagagcacaa
     2161 ttctcggagt tggtctggga a
//
LOCUS       DAOOYB010000389         2162 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_64409_length_2162_cov_10.194115, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000389 DAOOYB010000000
VERSION     DAOOYB010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64409_length_2162_cov_10.194115"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="PL056_13800"
     CDS             complement(<1..242)
                     /locus_tag="PL056_13800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48134.1"
                     /translation="MKKVVKTLVVLFFILFVAVGCDNKNNVQKDDEGNLPDGEVTDDP
                     DVKDKDENGEFITAGANNGNDKGEGDYGDSGNTGNSG"
     gene            complement(331..>2162)
                     /locus_tag="PL056_13805"
     CDS             complement(331..>2162)
                     /locus_tag="PL056_13805"
                     /inference="COORDINATES: protein motif:HMM:NF028231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HPM48135.1"
                     /translation="NLVDKADNLTTLEDIYAPYKPGRKTRADKAIEAGLLPLAEFVMQ
                     NNPDFETILNKAIEFVCEKFATAEEVVSGVKDIIAQNIADDLKVRTYVRSRMKSGIIS
                     SKVKRGKKEEGVVFSDYFDFSEPVSKIAPHRVMAINRGEKEAILNVTISPVEDFEEIS
                     ARISATFFNKRTGAFFNDCIVESYDRLLSKSIETEIFNEIRDLAIEASLSFFHSNLEQ
                     ILLSPPFGEKPVIGIDPGIRTGCKAVLLDGFGNYIDSTVLHLSTDKSQALKIKGWIEK
                     YNVKGIAVGSGTFGRETHEILSGYFGADAAVVLVDEDGASIYSASGTAREEFPDIDLT
                     VRGTISIGRRFQDPLSELVKIAPESLGIGQYQHDIPAKMLSEKLTRTVEWAVNKVGAN
                     LNTASPYLLSYISGLDSKKAKEISSLRKQNGRFTNIDELKKVKGIGAKTFEQCAGFLR
                     ILDGTEILDSTGVHPESYKIVKKAAKLCGTTVEELLKAPSIIKSNTDAKEIVPESVID
                     ELRKKGLDPRKKFETVQFSDEIRTITDLKTGMTLNGVVDNILAFGAFVDIGIKDKGLV
                     HISEIANEFVKNPADYLKIGQIVKVKVLSIDLERKRISLSMKN"
BASE COUNT          553 a          504 c          385 g          720 t
ORIGIN      
        1 cctgaattgc ctgtattgcc tgagtcgccg tagtcacctt cgcctttgtc attaccattg
       61 tttgcaccgg cagtaatgaa ttcgccgttt tcatccttat ctttcacatc cggatcgtct
      121 gtaacctcac catccggcag attgccttca tcatctttct gcacattatt cttattgtca
      181 caaccgactg caacaaaaag aatgaaaaac agcacaacca gtgttttaac aaccttcttc
      241 atttcgccct ccattcggca attaatcaaa ataacggaca tttagtgtat tgtactgtgg
      301 ttttttattt gagcaagggg aaaaatgttt tcaattcttc atcgaaagcg atattctttt
      361 ccgttcgagg tcaattgaga ggactttgac ttttacgatt tgtcctattt taagataatc
      421 ggcagggttt ttcacaaatt cattagcaat ttcagatata tggacgagtc ctttgtcttt
      481 tatgccgata tcgacgaacg ctccgaacgc gaggatgttg tcaacaactc cgttaagtgt
      541 catgccggtt ttgaggtctg tgatcgtcct tatctcatcg gaaaactgga ctgtttcaaa
      601 tttctttctg gggtcaagcc ctttttttct gagttcatca atgactgatt ccggcacgat
      661 ctcttttgca tcagtgttgc tttttatgat cgacggggct tttaaaagct cttcaaccgt
      721 tgtgccgcac agtttcgccg ctttttttac aattttataa ctttcagggt gaactccagt
      781 tgaatcaagg atctcagttc cgtcaagtat ccttaagaaa ccggcacact gctcaaatgt
      841 ctttgcccct atccctttta cttttttaag ttcgtcaatg ttggtaaatc ttccgttctg
      901 ctttcttaaa ctgcttattt ctttagcttt tttggaatca agtcccgaaa tgtagctaag
      961 cagataagga cttgccgtgt tcaagttcgc ccccacttta ttaactgccc attcaacagt
     1021 acgcgttagt ttttctgaaa gcattttcgc aggaatgtca tgctgatact gccctattcc
     1081 aagcgattca ggtgcgatct tgacaagttc tgagagcgga tcctgaaatc ttctcccgat
     1141 cgagatcgtt cctctcacgg tgagatcaat gtcaggaaat tcttcccggg cagtaccgct
     1201 tgcactgtaa attgatgcgc cgtcttcatc aacaagaaca actgcggcat ctgcaccaaa
     1261 gtaaccactt aatatttcat gcgtttctct gccgaatgtt ccacttccga ctgctatccc
     1321 tttcacatta tatttttcta tccacccttt tattttcaat gcctgggatt tgtcggtgct
     1381 aagatgaaga actgtggaat caatataatt cccgaaaccg tcaagaagaa ccgctttaca
     1441 gccagttctg atgccgggat caatgccgat gactggcttt tctccaaagg gcggcgaaag
     1501 aagtatctgc tcaagattgc tgtggaaaaa agacaatgat gcttcaattg caagatctct
     1561 tatctcatta aatatttcag tttcaatgct tttactcaag agccggtcat aactttcaac
     1621 aatgcagtca ttaaaaaacg cacctgttct tttattaaaa aatgttgcgg aaatcctcgc
     1681 tgatatttct tcaaaatctt caacaggaga aatcgtcaca tttagaattg cttccttttc
     1741 accgcggttt attgccatga cccgatgagg tgctattttt gaaaccggct cggaaaaatc
     1801 aaaatagtca ctgaaaacaa caccttcctc ttttttaccc cgtttgacct ttgaagaaat
     1861 tatgccgctt ttcattctgc ttctgacata tgttcgcact ttcagatcat ctgcaatgtt
     1921 ctgagcgatt atatctttca ctccgcttac cacttcttca gcagtggcaa atttttcaca
     1981 aacaaattca atggctttat ttaaaattgt ttcaaagtca gggttatttt gcatcacaaa
     2041 ttcagccaga ggaagaagtc ccgcctcaat tgctttatcc gcccttgttt ttctacccgg
     2101 cttatatgga gcataaatat cttcaagagt tgtaaggtta tccgccttat cgacaagatt
     2161 cc
//
LOCUS       DAOOYB010000390         2125 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_66060_length_2125_cov_11.970048, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000390 DAOOYB010000000
VERSION     DAOOYB010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66060_length_2125_cov_11.970048"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            200..1513
                     /locus_tag="PL056_13810"
     CDS             200..1513
                     /locus_tag="PL056_13810"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPM48136.1"
                     /translation="MKRMLELKLQTWKKSPVHKPIVLKGARQVGKSFLVREFGKSFNS
                     FVEVNFDFSEKYASIFELDLDPERIIKEISFLKKTAIVPGETLLFLDEIQECPNAIKA
                     LRYFYEMMPDLHLIAAGSLLEFALENIGLPVGRVTPMYLYPMSFKEFAQARNTGDILF
                     ESAKYFPEEIPDVFHSKLLKLLGEYMVVGGMPEVVFSWINEENFTRCREIQNEIIEVY
                     KSDFMKYAKKHQVKYVDLLYSSVHTTVGRKFTYSSIGQDFKSRELKPALDLLIKADVI
                     HKISHSSFSTIPLKAGEKPDHFKLIMSDIGLMQAMLNNDVSEWIIRPQDATQNFGSVT
                     EAFVGQEILANASPMKRTELFYWARSKRGANAEVDYLIEKENAVIPIEVKAGKSSRMV
                     SMEMFLNEKPEVRYGIHLSILNNRLKDKIHRFPLYLAGSLADISD"
     gene            complement(1673..>2125)
                     /locus_tag="PL056_13815"
     CDS             complement(1673..>2125)
                     /locus_tag="PL056_13815"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPM48137.1"
                     /translation="AGLIDVNLDSVYTYLHGRNYDDSKEEQVNKGVYLEYMPEHQLIF
                     NLMVDFNWKYKWLPVTTLMFWGEMSFGEVIYVMKSAPAETDPYSTDYFETASLHDPIM
                     LNLKISQEIWDYFKIWFAVKNLLDDYRADPFNPGAGRSFFVGAAFKYE"
BASE COUNT          674 a          386 c          414 g          651 t
ORIGIN      
        1 tcaccatctt cgtcacttca acatccattt tggaaaatcc gaaccgtttt ctgccgagat
       61 atttttatga ttcaaacctt gacttttgta cattttttaa atttttaacc ttgatttttg
      121 tgcattttac aatgttttaa ccttgacttt tgtacattga atgagataat tgcctgtatt
      181 tattgatttt gaggtggcca tgaaaagaat gcttgaatta aaattgcaaa catggaaaaa
      241 aagtcctgtt cacaagccga tagttttaaa gggggcaagg caggtgggga aaagttttct
      301 ggtcagagaa tttggaaaat cttttaattc atttgttgag gtaaatttcg atttttcaga
      361 aaaatacgca tctatttttg aactggatct tgaccccgaa agaataatta aagagatctc
      421 atttttaaaa aagactgcta tcgttccagg agagacactt ctttttcttg atgaaattca
      481 ggaatgccct aatgccataa aagcactgag atatttttat gagatgatgc cggatctcca
      541 tttaatagca gccggctctc tactggaatt cgcccttgaa aacatcggac ttcctgtcgg
      601 cagagttacg ccgatgtatt tatatcccat gtcttttaaa gaatttgctc aagcaaggaa
      661 cactggtgac atcctttttg agtcggcaaa atattttcct gaagaaattc cagatgtttt
      721 tcactcaaaa cttcttaaac ttttaggcga atatatggtt gtagggggaa tgcctgaagt
      781 tgttttttca tggatcaatg aagagaattt tacaagatgc agagaaatac agaatgaaat
      841 tatcgaggtt tacaaatcag atttcatgaa atatgcaaaa aaacatcaag ttaaatatgt
      901 tgaccttctt tactcatcag tccatacaac cgtgggcaga aaatttacat attcatctat
      961 cggtcaggat tttaaatcca gagaactgaa gccggcactt gacctgttga tcaaagcaga
     1021 tgtaattcac aaaataagcc attcgtcatt ttctacaatt cctttgaaag caggagaaaa
     1081 accggatcat tttaaactta ttatgtcaga tatcggactt atgcaggcaa tgctgaacaa
     1141 cgatgtttct gaatggatca tcagaccaca ggacgcaact caaaatttcg gaagcgtaac
     1201 agaggcattt gtgggtcagg aaattctggc gaacgcttct cctatgaaac ggacagagct
     1261 tttttactgg gcacggagca aaagaggggc aaatgctgaa gttgactatc tcattgaaaa
     1321 agaaaatgct gtcatcccta tagaagtaaa agccggaaaa tcttcaagaa tggtcagcat
     1381 ggagatgttt ttaaacgaaa aaccggaagt tagatacgga atacatcttt caattcttaa
     1441 caaccggctt aaagataaaa ttcacagatt tcctctttat ctggcaggaa gcttagctga
     1501 tatctccgac tgaatcttgc ttatttttaa gtcaacggaa aacaagtgaa aacgagaata
     1561 atattagtat gttattgaga tttaatgcat gaagcatatg tagttatcgg ggtcttgccc
     1621 cttttggttt ttctgtcaaa agcgacatat atcttcattt ttacctcaaa agttactcat
     1681 acttaaaagc agctccgaca aaaaagcttc tgccggctcc ggggttgaag gggtctgcgc
     1741 ggtaatcgtc aaggaggttc ttaactgcaa accagatttt gaaataatcc catatttcct
     1801 gagatatttt cagattgagc atgataggat catggaggga tgcggtttca aaataatcgg
     1861 ttgaatatgg atcggtctcg gcgggagcgc ttttcatgac atatataact tcgccaaaac
     1921 tcatttcacc ccagaacatg agcgttgtca cagggagcca cttatacttc cagttgaaat
     1981 caaccatcaa attgaaaatg agctgatgtt cgggcatata ttcaagataa acccctttat
     2041 tgacctgttc ttccttagaa tcgtcataat ttctgccgtg aagataggta tagaccgaat
     2101 ccaagttgac atcaataagc cctgc
//
LOCUS       DAOOYB010000391         2120 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_66307_length_2120_cov_9.329782, whole genome shotgun sequence.
ACCESSION   DAOOYB010000391 DAOOYB010000000
VERSION     DAOOYB010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66307_length_2120_cov_9.329782"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..647
                     /locus_tag="PL056_13820"
     CDS             78..647
                     /locus_tag="PL056_13820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48138.1"
                     /translation="MSVQKLLFVSVFLLFSTIYGVEKTESPQNFNVSVNGVYYSPEFL
                     MMEPTGGASLIFNYKSFRTSFFFGGTNYWEQTIYFFQSNIGGQYDFIKSEKMDAIIFS
                     EFNFNIVTGTFHKWDWPDDESPFFETTSLVVSTSTGFEYSVGNKNLKFNAGTKIYLPV
                     YLVFRFEDDLGFGIGYLLPGIEIYTGLKF"
     gene            760..2118
                     /locus_tag="PL056_13825"
     CDS             760..2118
                     /locus_tag="PL056_13825"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM48139.1"
                     /translation="MLIRFVVENIFSFDEKKEFQMIPLKTLKTLKHHVYDMKKLSLLK
                     MASIYGANGSGKSNLIKSIGLLQKLVLNEINADEIKSSIFKFHDPEKTKQILAVEFFQ
                     EGTPFYYGLEVKDGIIVTEELYLSGLGKKDKLIFERKTDESMRTSIDFSEEYENTEES
                     RVLKNILITEFVKPDETIIGLASRRENIFFDNVKKAQRWFEALLIITPTSKLALNPIL
                     TGLNPLLKKEELEKVVSEMNFGIKNILTEKTELMEYLGKNDLDQAEGIINKVEKSKEK
                     SIRLINKNKSIDVSIIKEEDKYFVLKPVFRHVGKMDREVAFDIKEESDGTIRIIDLIP
                     AFIIAAKFQHVIIIDEVERSLHPILIKELVNEFSASESTQGQLIFTTHESNLLDQEIF
                     RQDEIWFTEKDQNASTDIYSLSDFKEHKTIDIRKGYLCGRYGSIPFLSNLKDLGWLRN
                     VN"
BASE COUNT          747 a          330 c          385 g          658 t
ORIGIN      
        1 agatctcatt caaagcgata tctaacaaat atctccttaa acacggtggg tagttaaaaa
       61 ttagaccgga gtggattttg agtgttcaaa aactgctgtt tgtttcagtt tttcttcttt
      121 tctcaacaat ttacggagtt gaaaaaacag aatcaccgca aaatttcaat gtctcagtaa
      181 atggtgttta ttattcgcct gaatttttga tgatggaacc taccggtgga gcaagtctaa
      241 tatttaacta caaatctttt cgaacatctt tcttttttgg cggaacaaat tactgggagc
      301 agacgattta ttttttccag tcaaatatcg gcggacagta tgattttatc aagtctgaaa
      361 aaatggatgc aattattttc agcgagttca attttaacat cgtgacagga acctttcaca
      421 aatgggactg gcctgatgac gaaagcccgt ttttcgaaac aacctccctc gtagtttcaa
      481 catcaaccgg atttgaatat tcagtcggga ataaaaacct gaaattcaat gccggaacaa
      541 aaatttatct gccggtttat cttgttttta gatttgaaga tgatctcgga tttggaatag
      601 gatatcttct ccccggaata gaaatatata ccggattgaa gttttaacaa aaaccgtcct
      661 ttgcgtaatt tattcacatt aatttgtatt ttattctaat tttatttgca tttcatcgtt
      721 tacatgatat ctttgcgtga aaacttcacg gaggaagaca tgttaattag atttgttgtt
      781 gagaatattt tctcttttga tgaaaaaaaa gagtttcaaa tgattccttt gaaaacactc
      841 aaaaccctta aacatcatgt ttatgacatg aaaaaactga gtttgctaaa aatggcttct
      901 atttatggag ccaatggctc aggtaaatcg aacttaatta agtcaattgg attgctgcag
      961 aaacttgttc tcaatgaaat aaatgcagat gaaattaaaa gctcaatatt caaatttcac
     1021 gatcctgaaa aaacaaaaca gattcttgcg gttgaatttt ttcaggaagg cacccctttt
     1081 tattatggat tggaagtgaa agatggaatt attgtcacgg aagaacttta tttatccgga
     1141 ctgggaaaaa aggacaagct gatctttgaa agaaaaactg atgaatcgat gagaacctca
     1201 attgattttt ctgaagagta tgaaaatact gaagaaagtc gtgtgcttaa aaacattctc
     1261 attacagaat ttgtaaaacc tgacgaaaca ataatcggat tggcaagcag aagagaaaac
     1321 attttttttg ataatgtaaa aaaggctcaa aggtggtttg aagcactttt aattatcacc
     1381 cctacttcaa agctcgctct taatcctata ctaactggac taaatccttt gctaaaaaaa
     1441 gaagaactgg aaaaagttgt ctcagagatg aatttcggga ttaagaatat tttaactgaa
     1501 aaaacagaac tcatggaata tttaggcaaa aatgatcttg atcaagctga gggcataata
     1561 aataaggttg agaaatcaaa agagaaaagc atccggttaa ttaacaaaaa taaaagcatc
     1621 gatgtttcaa taatcaaaga agaagacaag tattttgtcc tcaagccagt cttcaggcat
     1681 gttgggaaaa tggatagaga ggtcgctttt gacataaaag aagaatcaga cggaacaata
     1741 agaataattg atctaattcc tgcttttata atagcagcta agtttcaaca tgttattata
     1801 attgatgaag ttgaaagaag tttgcatcca atcctgataa aagaactggt caatgaattt
     1861 tctgcatccg aatcaaccca gggacagctt atttttacga cacacgaatc gaaccttctt
     1921 gatcaggaaa ttttcagaca ggatgaaatc tggtttactg aaaaagatca aaacgcctca
     1981 actgatattt attctctttc agattttaaa gagcataaaa caattgacat tagaaagggt
     2041 tatctttgcg gaaggtatgg ctccatcccg tttttatcaa atttgaaaga tttggggtgg
     2101 ctaagaaatg ttaattaaga
//
LOCUS       DAOOYB010000392         2118 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_66401_length_2118_cov_7.547261, whole genome shotgun sequence.
ACCESSION   DAOOYB010000392 DAOOYB010000000
VERSION     DAOOYB010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66401_length_2118_cov_7.547261"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(223..1710)
                     /locus_tag="PL056_13830"
     CDS             complement(223..1710)
                     /locus_tag="PL056_13830"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HPM48140.1"
                     /translation="MRKNGNRFLDFLFFCSVAYILMSFAGCGGNEYSGDKIWEGNYEI
                     KTKSDVDKLRDYKEVTGKIFIRSELDVTELSLNTVKLAKEIYMKANNNVKSFSMPLLE
                     KTEKIELGGRFNYETTSLESVSFPNLKNGILWIEGSALVNSIEIPLLESGGIYISGCA
                     DLETVSAPLLENGMVYITNNSSLKEISCPVLKTTGTGSFLISYNNNLKSFEFPELREM
                     NSGGISDNSILESFSLPKLEKMGGNPKLSTNNSLKEIFFPELKEAREIKIEKNNALKL
                     VDLPKLVSTENGIEILHNPALEFINMPKLQIVGSAEHESFGYFYIDINLSLKSIDFSS
                     LEKVVSSFRMTTNWALPSSYIYDLLDRIVVGGTIDYQLNSWKDPDTNYQWSKIAYKQK
                     DEYGYGNSIKMNFEDASGYCDALNEEGFEDWRLPTIEELETLIRGDCTGFRSCSKLGD
                     TGLFWSSTPVNDEKMWTYTFVSNGKASIGRGEKDELHGQIRCVRE"
BASE COUNT          650 a          444 c          289 g          735 t
ORIGIN      
        1 aagcacgcac ttcacggtcg tcaaaaaaac ggattgaaat tttctgctga tcccggtttg
       61 tcatctgact ctttatgact cctaaatctc cgaataaact gaaatattat atttaaaatg
      121 accggaatta caaaattctc aatagcggca attaagcgtc aggatatcat tcaagcggcg
      181 gcttcttcat ctcttttttc ataaaacctg agtttctgaa ctttattctc ttacacatct
      241 tatctgtccg tgtaattcat ctttttcccc tcttcctatc gatgctttcc cattactcac
      301 aaaagtataa gtccacattt tttcatcatt aactggtgtt gatgaccaaa ataaaccagt
      361 atctcctaat ttagaacatg aacgaaaacc ggtgcaatct cccctaatta aagtttccag
      421 ttcctcaatt gtcggcaatc gccagtcttc aaatccttct tcattaagtg cgtcacaata
      481 acctgaagca tcctcaaaat tcatctttat agaattcccg taaccgtatt cgtctttttg
      541 cttatatgcg attttagacc attgatagtt tgtgtccgga tctttccatg agttaagctg
      601 ataatcgata gtaccgccga caactattct atccaacaag tcataaatat atgaactggg
      661 aagcgcccag tttgtggtca ttctaaaact acttacaact ttctccagag aagaaaagtc
      721 aattgattta agtgacaaat taatatctat ataaaagtaa ccgaaagatt catgctcagc
      781 agatccgaca atttgaagtt tcggcatgtt aataaattca agtgccggat tatgtaaaat
      841 ttcaatacca ttttctgttg aaaccagttt tggcagatca actaatttaa gagcattatt
      901 cttttctatt ttaatttctc ttgcttcttt aagctcagga aagaaaatct ctttcagcga
      961 attgtttgta cttaacttgg gattgcctcc cattttttca agcttcggta gagaaaaaga
     1021 ttcaaggatg ctattgtcgc tgatccctcc cgaattcatc tcccttaatt caggaaattc
     1081 aaaagatttc aaattattgt tgtatgaaat aagaaaacta cccgtaccag ttgttttcag
     1141 gactggacag ctgatttcct ttaaagaact attgtttgtt atatatacca ttccattttc
     1201 aagcaatggg gctgaaaccg tttctaaatc agcacatcca gatatatata ttcctccact
     1261 ttcaagtaac ggaatttcaa tactattaac caatgcagat ccttcaatcc acaatattcc
     1321 atttttaaga tttgggaacg aaacactttc aagggaagtg gtttcatagt taaatcttcc
     1381 acctaattca attttttctg ttttttcaag gagaggcatt gaaaaacttt ttacattgtt
     1441 atttgccttc atatatattt cttttgccag cttaactgta ttcagactta attcagtgac
     1501 atcaagctca gacctaataa aaatttttcc agtgacctcc ttgtagtctc tcaacttatc
     1561 aacatctgat tttgttttta tctcgtaatt tccttcccag atcttatctc cagaatattc
     1621 atttccacca caaccggcaa aagacattaa tatataagct acagagcaaa aaaataaaaa
     1681 atctaaaaat ctgttaccat tttttctcat atttccctcc tgtcatgatc gttaaagtta
     1741 aaaaaatata atctcagttt tgttttttgc aatattttcc acttttttct tctccagtgt
     1801 ctcctgtgtt acctgtatct cctgtatctt caggttcaat acataaattt gaaacacact
     1861 cataaccttc atcacaagtg ttatcaggaa aacatggacc gcccttggaa ccttgcggct
     1921 tggaattctc acccgttgaa gtttctccgc atgagataaa aaaacaatgt taataatcct
     1981 gcgataaaga aaaacttttc catgatttga ccgcttatca aatttaataa aaaaatcctc
     2041 tctctttatt tctttcttta attagagccg attgcaaaac tcctgaatat ctgaaaagtg
     2101 tctgactcgt atttctga
//
LOCUS       DAOOYB010000393         2107 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_66929_length_2107_cov_5.853801, whole genome shotgun sequence.
ACCESSION   DAOOYB010000393 DAOOYB010000000
VERSION     DAOOYB010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66929_length_2107_cov_5.853801"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            153..1178
                     /locus_tag="PL056_13835"
     CDS             153..1178
                     /locus_tag="PL056_13835"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HPM48141.1"
                     /translation="MIKRILLMISLVFLFLSLFSAEKDRLAVMDVADEAKLFNKTTIA
                     NVTDYIFTKLQGTKIYWMVPKADRDTALEQAIEETVKGSRKECVDEKCQLSLVAQLQA
                     NFLINTKIKKLYEGTCNISISKFDVEKRAGVDSWTTDFNCSEKGVFEAINGFDFLKKS
                     KLENKKEKETKEDNSDKNACIYAREKNSLEIWEKYISKYPKGECAFQALEEIRSKKGK
                     EWSKIVSTKMNWSDAKNYCESDGWRLPTISELRTLVKNCPLTQKDGECKINDECLSDS
                     CESQACKGCMYSNDGRYSVFKDSEYLWSSSVVQYKEKNAWYLGFGDGASIYNDPKSSK
                     HKFRCVK"
     gene            1384..>2107
                     /locus_tag="PL056_13840"
     CDS             1384..>2107
                     /locus_tag="PL056_13840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48142.1"
                     /translation="MKRFFLLFLLLIPLSLFSEKIMSDEEFRKTLYGSGGVTDNSGDL
                     KNGLSSQDVVSKIIEVFNATQVINDAYLYRLEGFLKNAYVVLNEVVAAGSVSVWGADY
                     YKRLSHTYGLAIKETEFYNSMLKIFKQNILNLSAARRSDFLYYLIELNSLGGDRAIFF
                     LIVLLEKNGSVEYSLEDRVFYTMLRDYALWLKFSALVADHFQAVKSIKGDNIYEKRYE
                     SDKALNKLNESYEDYFFNLRVPS"
BASE COUNT          665 a          302 c          451 g          689 t
ORIGIN      
        1 gaaaatggtt cggattttcc aaaatggatg ttgaagttgc gaagatggtg acaaaactgc
       61 tttaaatttc ctctaaattt gccagattta gaagtttaat atataatcgg ttttaagatt
      121 tttgacacag atttaatctt gcgaggttga tgatgataaa acggattttg ttaatgattt
      181 cattggtttt tttgtttctt tcgctctttt ctgcggaaaa agaccgtctt gcggtaatgg
      241 atgttgctga tgaagcgaaa ctgtttaaca agacaacaat tgccaatgtt acagattata
      301 ttttcacaaa gctgcaggga acgaaaatat actggatggt tccgaaagcg gatagagaca
      361 cagcgctcga acaggcgatt gaagaaaccg ttaaagggtc aagaaaagaa tgcgttgatg
      421 aaaaatgcca gttgtcacta gtggcacagc ttcaggcgaa tttcttaata aatacaaaga
      481 ttaagaaact ctatgaagga acctgcaaca taagcatcag caaattcgat gttgaaaaaa
      541 gagccggagt agattcatgg acaactgatt ttaattgttc tgaaaaaggt gtttttgagg
      601 caattaacgg gtttgatttt ttaaaaaaaa gtaaactgga aaacaagaaa gaaaaggaaa
      661 cgaaagaaga caattcagat aaaaatgcct gtatctatgc aagggagaaa aacagtttag
      721 aaatatggga aaaatatatc tcaaaatatc caaaagggga gtgtgcattt caggcgttgg
      781 aagaaataag aagtaaaaag gggaaagagt ggtcaaaaat tgtttccact aaaatgaatt
      841 ggagtgatgc aaaaaattac tgtgaaagtg atggttggcg attgccaaca atatcagaac
      901 ttaggacact tgtgaaaaat tgtcctttaa cccaaaaaga cggggagtgt aaaataaacg
      961 atgaatgttt gtctgattca tgtgaatcac aggcgtgtaa aggatgtatg tattctaacg
     1021 atggcagata ttctgtgttt aaagattctg agtatttatg gtcttcatct gttgttcagt
     1081 acaaagagaa aaatgcctgg tatttaggtt ttggagacgg agctagcatt tataatgatc
     1141 ccaaaagctc aaaacataaa ttccgttgtg ttaagtgagg tcgctgcttg aacagtgaac
     1201 agagagaaat gtcccgcaaa ttttctgagt gggaaaattt gtccccgatc ccctttcatc
     1261 tgttaaaacc tatgccatcc aatcccggcc gccatatggt tttctttatt tacttaaaat
     1321 ttccaatttt aagctaattc attaaaatgt ttctgttgta attaattttt tgtaggggtt
     1381 cccatgaaaa gattttttct tttgtttttg ctcttaatac ctctttccct tttttcagaa
     1441 aaaattatgt ctgatgaaga gtttcgcaag accttgtatg gttccggtgg tgtaactgat
     1501 aattccggtg acttaaaaaa tggtctttcc tcccaggatg tagtttctaa aatcattgag
     1561 gtttttaatg caacgcaggt tattaatgat gcatatcttt atcgtctgga aggttttctt
     1621 aaaaacgctt atgttgtttt gaatgaggtt gttgccgctg gatctgtttc ggtatggggt
     1681 gcggattatt ataaaaggct ttctcatacc tatgggctag caattaaaga gaccgagttt
     1741 tacaattcta tgcttaagat tttcaagcag aatattttaa atttgagtgc tgctcgtcgt
     1801 tctgattttt tgtattattt gattgagctt aactcccttg gtggcgatcg ggctattttt
     1861 tttcttattg tgctgcttga aaaaaatggt tctgttgagt actctttaga ggatcgagtt
     1921 ttttatacga tgcttaggga ttatgcttta tggcttaaat tttctgcatt agtagcggat
     1981 cattttcagg cagttaagtc cattaaggga gacaacattt atgaaaaacg ctatgagtct
     2041 gacaaggcat tgaacaagct gaatgaaagt tatgaggact atttctttaa tttaagagtg
     2101 ccctctt
//
LOCUS       DAOOYB010000394         2103 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_67128_length_2103_cov_5.265137, whole genome shotgun sequence.
ACCESSION   DAOOYB010000394 DAOOYB010000000
VERSION     DAOOYB010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67128_length_2103_cov_5.265137"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..83
                     /locus_tag="PL056_13845"
     CDS             <1..83
                     /locus_tag="PL056_13845"
                     /inference="COORDINATES: protein motif:HMM:NF017847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HPM48143.1"
                     /translation="KAGAYKLEGKEYVVKDGDIINFRFNV"
     gene            76..1644
                     /locus_tag="PL056_13850"
     CDS             76..1644
                     /locus_tag="PL056_13850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48144.1"
                     /translation="MSKQNTVYIISIFTVFILFVILKMFSLNPYAGDEHIYIYQAKLV
                     SEGFLPYKDFSLAHPPFHTIITAIIIKSVGYGFYTTRFFPFLWTIISGTLLTLLTKNE
                     LGKAASVAGLAIFLFSYDLLRVSSHSTGVNCAVALTLAGILFLRKKCSVPAAIFFSLA
                     LMTRFYVFPVISAALIYSLFSDRSGTFRTIKFLVFFLFSSFLFLGLFCGFENLWTNFF
                     MFHFNKTSMSGETLSAVTDKFMFHNFTLISLFILSIPLYFIKMDKAKSLILLSIICAA
                     SMFALLFSMNRVWSYYFIPALPFAAIAGGWVVSEWIESIRNKRNIAVTAVTVLIFIVV
                     SVLSFKLESRLDYWDKEMAKPLENRTHFYKWRDGFLPGFLNTAVNYTLWNEKRVIGNR
                     YFFFNYYLWHESRVLEIAEEAVEIIIKNTSENGEISGDSGTVPLFALLSGKRIAGNDP
                     DTNIQKYRSGVFKASDLIFDIDTPKTEMIILRKKFGIAGLSEIKELVARKYNLINELK
                     DPSGITLLFYKRKN"
     gene            complement(1646..>2103)
                     /locus_tag="PL056_13855"
     CDS             complement(1646..>2103)
                     /locus_tag="PL056_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010518560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HPM48145.1"
                     /translation="SVKDSISVGFDFSLVSAEIEEIHTFVDIDNDGIDDLKDNCPMVA
                     NANQLDSDKDGLGDVCDCSPSHSRYDGELCNGIDDTCDGLTDDSFAELGNSCGTGMCG
                     GGAMVCSDDGLSEICSTMPGGPSDSSNPEECNGIDDDCDGETDEEEVCG"
BASE COUNT          606 a          435 c          373 g          689 t
ORIGIN      
        1 aaaaagccgg tgcctataaa cttgaaggaa aggaatatgt cgtcaaagac ggcgacatca
       61 taaatttcag gtttaatgtc taagcagaac acagtttata ttatttcaat atttacagtt
      121 tttatccttt ttgtaatcct gaaaatgttt tcgcttaacc cctatgcggg cgatgagcat
      181 atttacattt atcaggcaaa acttgtttca gaaggtttcc tgccgtataa agatttttcc
      241 cttgctcacc cgccgttcca tacaatcata acggcaataa taataaaatc agttggttat
      301 gggttttaca ccacgaggtt ttttcctttt ttgtggacta tcatttcagg tacactgctg
      361 acacttctta caaaaaatga gctcggaaaa gctgcatctg ttgcaggcct tgcaattttt
      421 cttttttctt acgatctttt aagggtctct tcccattcaa ccggagtaaa ctgtgccgtt
      481 gcgctgaccc ttgccggaat tctttttcta cgcaaaaaat gctctgttcc tgccgcaata
      541 ttcttttcac ttgctttaat gaccaggttt tatgtttttc ctgtgatctc agccgctttg
      601 atatactctt tattttcaga tagatccggc acattcagaa caattaagtt tcttgtattt
      661 tttctgtttt catctttcct ttttttagga ttgttttgcg gttttgagaa tttatggaca
      721 aattttttca tgtttcattt caacaaaacc tcaatgagtg gagaaaccct ttcggcagtc
      781 actgataaat tcatgtttca taactttact ctgatctctt tgttcatctt gagcattcct
      841 ttgtatttta taaaaatgga caaagccaaa tcactgatat tactgtcaat aatttgcgcc
      901 gcatcaatgt tcgcactcct tttttcaatg aaccgtgtct ggtcatatta tttcattccg
      961 gcacttcctt ttgctgcaat tgcaggggga tgggttgttt cagaatggat cgaaagcatc
     1021 agaaataaaa gaaatattgc agtcacagca gtaacggttt taatattcat cgtcgtttct
     1081 gttttgtcat ttaaattgga aagccgtctt gattactggg ataaggaaat ggcaaaacca
     1141 cttgaaaaca ggacacattt ttataaatgg cgtgacggat ttcttcccgg atttctgaat
     1201 actgcggtaa attacacatt gtggaatgaa aaaagggtga tagggaacag atattttttc
     1261 tttaactact acctctggca tgaaagcaga gtccttgaaa ttgctgaaga agcagttgaa
     1321 ataataatta aaaacacctc tgaaaacggt gaaatatccg gagattcagg aactgttccc
     1381 ctttttgctc tgctttcagg aaaaaggatt gccggaaatg accccgacac aaacattcag
     1441 aaatacagat cgggcgtttt caaggcatct gatctcattt tcgatattga tactcccaaa
     1501 accgaaatga taatacttag aaaaaagttc gggattgcag gacttagtga aataaaggaa
     1561 cttgttgcca gaaaatataa tcttatcaat gagttaaaag atccgtcagg tattactctt
     1621 ttgttttata aaagaaagaa ctgaattaac cgcaaacttc ttcttcgtct gtttcgccgt
     1681 cacagtcatc gtctatgccg ttacactctt caggattcga actgtcgctt ggccctcccg
     1741 gcatggttga gcaaatttct gaaagcccat catccgaaca aaccattgca ccgccaccac
     1801 acattcctgt tccgcatgaa tttccaagtt cagcaaaact gtcatctgtc agtccgtcgc
     1861 aagtgtcatc aattccatta cacaattcac catcatatct tgagtgtgaa ggactgcagt
     1921 cacaaacatc tcccagtccg tctttatctg aatcaagctg attggcgttt gccaccatcg
     1981 gacaattatc cttcagatcg tcaataccgt cattgtcaat atcaacgaaa gtgtgtattt
     2041 cttcaatttc tgctgaaaca agcgaaaaat caaaaccgac ggaaatggaa tcttttacgg
     2101 aat
//
LOCUS       DAOOYB010000395         2099 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_67304_length_2099_cov_6.096869, whole genome shotgun sequence.
ACCESSION   DAOOYB010000395 DAOOYB010000000
VERSION     DAOOYB010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67304_length_2099_cov_6.096869"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="PL056_13860"
     CDS             <1..205
                     /locus_tag="PL056_13860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48146.1"
                     /translation="NKETVYSISVGSWNSEKWESDKESFTDVSNYEPNEMESQSKTIK
                     IGQQITSYLHKGDLDFWIIDMSE"
     gene            206..1117
                     /locus_tag="PL056_13865"
     CDS             206..1117
                     /locus_tag="PL056_13865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48147.1"
                     /translation="MGKIKILFIAVIFLNFFNLTSDDADPQNKFNLNIEYNKSSGVMP
                     SLDVLWNYNSFVFSSIYAGYYTKSEKKRLENFTESKYSIFSNTIVVGGEIVGFYVTKS
                     PFIFAISIGGEYKRILTEEFGYFLLNDERVIFENNIKLHTFFPFLEITIDKKSEYVEN
                     RFNLSFFPSYFLNFNQDISFKPIITGKGKKSSNNWQVPALEISDDLIFNLKVFSILLS
                     ADFMIWQAKYTMQTLRNSESGYFYGENDIKQTFLEYSVSINVILPFKIVGNVRPMVGI
                     GYSGSTVYFDEEKKQYNDYILNIGMYY"
     gene            1243..1374
                     /locus_tag="PL056_13870"
     CDS             1243..1374
                     /locus_tag="PL056_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017448833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48148.1"
                     /translation="MGKCKKMTPEAASRVQSSTAKKNEGKVEKGSFASRAQSSGTKK"
     gene            1447..1656
                     /locus_tag="PL056_13875"
     CDS             1447..1656
                     /locus_tag="PL056_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008150160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48149.1"
                     /translation="MDKKCIPYGSDVSGGQFRCRDCGEIITMPSRTSLPPCPYYKILP
                     HPLKCWENISGQGDAPKDPYPKKKN"
     gene            1674..1982
                     /locus_tag="PL056_13880"
     CDS             1674..1982
                     /locus_tag="PL056_13880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48150.1"
                     /translation="MNGSNNSIRIISDIGNVCEKFKIGKTGQTLEERFQGEYQQEYDN
                     IVEVYRNKKKEIVDFMEMFLIDHFMKSKEYSDKLQNEQVGGGEMADFDEYYVYVVYKL
                     "
BASE COUNT          761 a          302 c          399 g          637 t
ORIGIN      
        1 aaacaaggag accgtttatt caataagcgt tggctcttgg aatagcgaaa aatgggaaag
       61 tgacaaggaa tctttcacag atgtttcaaa ttacgaaccc aatgaaatgg agtctcaatc
      121 caagactatt aagattggtc aacaaatcac ttcttacctt cataaaggag atctcgattt
      181 ctggattatt gatatgagtg aataaatggg aaaaattaaa atattattta tagcggttat
      241 ttttcttaac ttttttaatt taaccagtga tgatgctgat ccgcaaaaca agttcaacct
      301 gaacattgag tacaacaagt cttcaggagt catgccgtcg ctggatgttt tgtggaatta
      361 taatagtttt gttttttctt caatttatgc tggatattat acgaaatccg aaaagaaaag
      421 acttgaaaac ttcaccgaaa gtaaatattc aatattctct aacacaatcg tagtcggtgg
      481 cgagattgtt ggtttttatg tcactaaatc gccatttatt tttgcgataa gtattggagg
      541 agaatataaa agaattctaa ctgaagagtt cggctatttt ctgctaaatg atgaaagagt
      601 catttttgaa aacaatataa aactgcatac ttttttccca ttcttagaaa ttacgattga
      661 caaaaagagc gaatatgttg aaaacaggtt taatctttct ttctttcctt catattttct
      721 aaactttaat caagatatct cttttaaacc aattattacg ggaaaaggaa agaaatcttc
      781 aaataattgg caagtcccag ctcttgaaat ttcagacgat ctgatcttta atctaaaagt
      841 tttttcaata cttctaagtg cagatttcat gatatggcag gcaaaatata caatgcaaac
      901 gcttagaaac tccgaatcag gatatttcta tggagaaaat gatatcaaac agactttttt
      961 agaatactca gtatcgataa atgttattct tcctttcaaa attgttggaa atgttagacc
     1021 gatggttgga attgggtatt ctggttcaac tgtttatttt gatgaagaga aaaaacaata
     1081 caatgattat atcttaaata tcggaatgta ttattgaaat aaatttcctt cctttttttg
     1141 aaaattttaa tccatctgtt gacttaagcc gaatggtgct taaaaaataa tcctgttcaa
     1201 ttatggaaca agatatttta aagtttttac agggagaaag tcatgggaaa atgtaaaaag
     1261 atgactccgg aagcagcttc aagagtccag tcttcgacgg caaagaaaaa tgaaggaaaa
     1321 gttgaaaaag ggagctttgc ttcaagagca cagtcatccg gcacaaaaaa atgaaaatgc
     1381 taaaaagcag tctggctcaa attccggatc gaacaagaaa acaactggta agtagggagg
     1441 agcaaaatgg acaaaaaatg tattccttat gggtctgatg tttccggtgg acagtttagg
     1501 tgtagagact gtggagaaat tattacgatg ccttccagaa cttctcttcc tccttgtcca
     1561 tattacaaga tcttgccaca cccattaaaa tgttgggaaa acattagtgg tcaaggtgat
     1621 gccccgaaag atccatatcc taaaaagaaa aactgatttg agtggaggca aaattgaacg
     1681 gatcaaacaa ttcgattaga ataatttccg acataggaaa tgtgtgcgaa aaattcaaga
     1741 tcggtaaaac agggcagact ttagaagaaa gattccaagg agaatatcag caagaatacg
     1801 ataatatagt tgaagtttac cgtaacaaaa agaaagaaat agttgacttc atggaaatgt
     1861 ttctgattga tcattttatg aaaagtaaag aatatagtga caaactccag aatgaacagg
     1921 tcggtggcgg tgaaatggct gattttgatg aatattatgt ttatgttgtg tataaattat
     1981 agggttctga aaaaaagtgt tttgcattat ataaaaagga gaaaagatgc aagtcatttg
     2041 gaataattga agttccccca aaaaaaattc agcagatttc taactgaaga ggaggagct
//
LOCUS       DAOOYB010000396         2097 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_67418_length_2097_cov_4.017140, whole genome shotgun sequence.
ACCESSION   DAOOYB010000396 DAOOYB010000000
VERSION     DAOOYB010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67418_length_2097_cov_4.017140"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="PL056_13885"
     CDS             <1..517
                     /locus_tag="PL056_13885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48151.1"
                     /translation="LGKMTWKADIPEQELKNCLSNLRAKKMFNGNNLKKYLFVIMLFL
                     LFGCDEESSEIYPSATSLYLYNNSNESAYIKGYKESDPDTLYYELIVPSDQVRSVGYM
                     DVTVNGKFFESDRLKIYKGGNLIYDVSAPRSLDMYYELFSDSIKLDAGDQTVIVAPIF
                     KWCGNQLCKDD"
     gene            539..1513
                     /locus_tag="PL056_13890"
     CDS             539..1513
                     /locus_tag="PL056_13890"
                     /inference="COORDINATES: protein motif:HMM:NF013217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48152.1"
                     /translation="MERIFFVVLVFMLVFFAIACGSDPKVGEEGGKCFEDNTCKGKLK
                     CVEDFCVDLGDMTDYPDVDSHNDIQDNDAVSDDDNENGGENPDLIDECDFSIEVMNKK
                     DLDSIVNCRVITGELIIEETDLEEVVLPNLEEVRDYFDIFENPKLKKISMPKLEYAGS
                     IFGIDMNPLLTEINFSKLNYVADNIVVWGNDSLKTLSFPSLKKADEGIYIDFNKTLES
                     IDAPLLESVKEFIEFDHNDSLRSFSFPVLESALGLSISRNSVLETFDMALFYDVQEIF
                     IVLNPQLPTGKAEALIEQIKEKGTSSYEYEICGNKDGTKCDFDWEDLD"
     gene            1587..2057
                     /locus_tag="PL056_13895"
     CDS             1587..2057
                     /locus_tag="PL056_13895"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HPM48153.1"
                     /translation="MAFDVGAVIFIILAMIAGYLAGGFREILKLVVLIGVFSLFKIPS
                     FEAAMKEFAGPKMYTGFYIIAFLTTYFAFYWMLFLSLKGLIKEREGTLGDINKTIGVI
                     AGFFRGIAILTVMVYILKALFKKNMLIELKPHTAESLFYNIASTVLEKIGLIFF"
BASE COUNT          656 a          297 c          438 g          706 t
ORIGIN      
        1 tcttggaaag atgacatgga aagcagacat cccggaacag gagttaaaga actgtttgag
       61 caatttgaga gcaaaaaaaa tgttcaatgg aaataattta aaaaaatatt tgtttgtaat
      121 aatgcttttt cttttatttg gctgtgatga ggaatcttca gaaatatatc cttctgccac
      181 atcgttatat ttgtacaata atagtaatga aagtgcttat atcaaggggt acaaagaaag
      241 cgatcctgac acactgtatt atgaactgat tgttccttca gatcaggtca ggtctgttgg
      301 ttatatggat gtgacagtaa atggtaaatt ttttgaatca gacagattga agatatataa
      361 aggcgggaat ttaatatatg atgtatctgc tccgagatcc ttggatatgt attatgagtt
      421 gttttcagat tcaattaaat tggatgctgg agaccagact gtaatagtag ctccgatttt
      481 taaatggtgc ggaaatcagt tgtgtaaaga tgactaatta ttttaaggaa tattaattat
      541 ggaaagaatt ttttttgttg ttttagtatt tatgctagtg ttttttgcga tagcttgcgg
      601 ttcagatcct aaagttgggg aagaaggggg aaagtgtttt gaagataata cttgcaaagg
      661 aaaattaaag tgtgttgaag atttttgtgt tgatcttgga gacatgactg attatcccga
      721 tgttgattct cataacgaca ttcaagacaa tgatgcagtc agcgatgatg acaatgaaaa
      781 cggtggagag aatcctgatc tgattgacga gtgtgatttt agcattgaag taatgaataa
      841 aaaagatctg gactcaattg ttaactgccg tgttatcaca ggtgagctga taattgaaga
      901 aaccgatctg gaagaagtag tgcttccaaa tcttgaagaa gtccgggatt attttgatat
      961 ttttgaaaat ccgaagttga aaaaaatatc tatgcctaag ctggagtatg caggaagtat
     1021 atttggaatt gatatgaatc cgttactgac agaaattaat ttttctaaac ttaactatgt
     1081 ggcggacaat attgttgtgt ggggcaatga ttctttaaaa actctttcat ttccgagcct
     1141 taaaaaagct gatgaaggaa tttatattga tttcaacaaa accctggaat ctatcgatgc
     1201 tccgcttttg gaaagtgtaa aggaattcat tgaatttgat cacaatgatt cactaagatc
     1261 attttcattt cctgtgcttg aaagtgctct cggactgagt atttcaagaa acagcgttct
     1321 tgaaacattt gatatggcgt tattttatga tgtccaggag atttttattg tcttaaatcc
     1381 tcaactgccc accggaaaag cagaagctct catagaacag atcaaagaaa aagggacttc
     1441 ttcctatgaa tacgaaatct gcggcaacaa agatggaaca aaatgtgatt ttgactggga
     1501 agatctggat tgaagttaaa ttgacatatt gttgatatat ttctgcaaat aagtaaaaat
     1561 atctcagata atttctgtgg tgaaatatgg cttttgatgt cggtgcagtg attttcataa
     1621 ttcttgcaat gattgcgggt tatcttgcgg gcggatttcg tgaaatattg aaactcgttg
     1681 ttcttatcgg ggttttctct ctttttaaga ttccttcctt tgaagctgcg atgaaagagt
     1741 ttgcaggacc taaaatgtac acaggttttt atatcattgc ttttcttaca acatatttcg
     1801 ctttttactg gatgctcttt ctttcactta aaggtctcat aaaggaaaga gagggaacac
     1861 tcggcgacat caacaaaaca ataggtgtga tcgccggttt tttcagaggg attgcgatac
     1921 ttactgtcat ggtctatatt ctcaaagcac tttttaaaaa gaacatgctc attgagttga
     1981 aaccgcacac cgcagaatcg cttttctaca atatcgcaag caccgtgtta gaaaaaatcg
     2041 ggctgatttt cttttaattt ttcatttttg ttattattct tcctgcgaaa tttgttt
//
LOCUS       DAOOYB010000397         2086 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_67935_length_2086_cov_6.326933, whole genome shotgun sequence.
ACCESSION   DAOOYB010000397 DAOOYB010000000
VERSION     DAOOYB010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67935_length_2086_cov_6.326933"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="PL056_13900"
     CDS             <1..404
                     /locus_tag="PL056_13900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48154.1"
                     /translation="FASIAYTPITFDNIEMFGPQVVLMFNSETIPNTTPGTEDYYNIG
                     GIGDDDIIIAIGEQFVSSDTIDVDSLCIAAIAFGKKMVVSNAFNVMADGGGGSITFSG
                     FDIPLYHPTETPLGDLTEMVKEYGLENICDK"
     gene            419..1795
                     /locus_tag="PL056_13905"
     CDS             419..1795
                     /locus_tag="PL056_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HPM48155.1"
                     /translation="MKINRTIFRQYDVRGHVNVDLTEDFAYSLARGCAAYYKKELPSI
                     KKVSIGHDARESSPLFASALIKGFNDSGLDTVFLGMVPTGLLYFSMFTLDVQASVMIT
                     GSHNPPEYNGFKINLVDLPVYGDGIQKIADIMEADDFIDAEVKGKNSEYNIIPDFEKY
                     ILKNIKIQNPEKLKIVVDSGNGVGGFVAVPILRKMGVNVISIFEEPDGTFPNHHPDPT
                     VEKYIQDMIKTVIAEKADFGIGYDGDADRIGLVDRNGKIIWGDKILTIFSRSILEEVP
                     GATIVAEVKCSKTLYDDIKKRGGKGIMWKAGHSLIKAKMKEVGAAFGGEMSGHVFFKH
                     RFFGYDCAIYSTFRLLEIVSKMDHVDFDKLLEGIPETFSTPEIRLEVDDLKKFGIVEN
                     VVKYFKNEGADVNDIDGVRVTFDDGWGLMRPSNTQNVIVLRVEADSLKRMNEIRELLE
                     RKLDEYNR"
     gene            1808..>2086
                     /locus_tag="PL056_13910"
     CDS             1808..>2086
                     /locus_tag="PL056_13910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011551635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HPM48156.1"
                     /translation="MSKALVMILAGGQGKRLAPLTLDRAKPAVPFGGTYRIIDFVLSN
                     FVNSGFMQIKVLTQFMSNSLNQHISRNWTLSPTFNQYVDSVPAQMRTGD"
BASE COUNT          637 a          401 c          481 g          567 t
ORIGIN      
        1 tctttgcttc gattgcatat actccaatta cttttgataa tattgagatg ttcggaccgc
       61 aggtggtgct gatgttcaat tccgaaacga ttcccaacac aacgccaggc acagaagatt
      121 actataatat cggcggaata ggagatgatg atataattat tgctatcggt gagcagtttg
      181 tttcttctga tacgatagat gtggattcgc tttgtattgc ggcaattgct ttcggcaaga
      241 aaatggtagt ttccaatgca tttaatgtta tggctgacgg tggcggtgga tccataactt
      301 tttccggctt tgacattccc ctttaccacc cgactgaaac tccgcttgga gaccttactg
      361 aaatggttaa ggaatacgga cttgagaaca tttgtgacaa atagaacagg gggcaaaaat
      421 gaaaatcaac aggacaattt tcagacagta tgatgttcgt ggacatgtca atgtcgatct
      481 gacagaggat tttgcataca gtcttgcaag gggctgtgcc gcatattata aaaaagaact
      541 tccttcaatt aaaaaagtga gcatcggtca cgatgcaagg gaaagctcac ccctttttgc
      601 tagtgcactc atcaaaggat tcaacgacag cggccttgac acagtatttc tcggaatggt
      661 tcccactggt cttctctatt tttcgatgtt cacgcttgat gtacaggcat ctgtcatgat
      721 aacaggaagc cacaatccgc ctgaatacaa cggttttaag atcaatcttg ttgaccttcc
      781 tgtttacgga gacggcattc agaagattgc cgacattatg gaagcagatg atttcattga
      841 tgccgaagtt aagggaaaaa acagtgaata taacatcatt cccgattttg aaaagtatat
      901 tctcaaaaac ataaaaattc aaaatcctga gaaattaaag atagttgtag attcaggcaa
      961 tggagttggc ggttttgttg cagttccgat cttaagaaaa atgggtgtaa atgtcatcag
     1021 tatctttgaa gagccggatg gaacttttcc aaaccatcat cccgacccga cagttgaaaa
     1081 atatattcag gacatgataa agacagtcat tgctgaaaaa gccgatttcg gtatcggtta
     1141 tgacggtgac gcagacagga tcggacttgt tgaccgtaat ggaaagatca tctggggtga
     1201 caagatcctc actatttttt caagaagcat ccttgaagaa gtgccgggtg caacgatcgt
     1261 tgccgaagta aagtgcagta aaacgcttta tgatgatata aagaaaagag gtggaaaagg
     1321 gatcatgtgg aaagcgggac attcgcttat caaagccaag atgaaagagg ttggagccgc
     1381 tttcggcgga gagatgagcg gacatgtttt tttcaaacac cgctttttcg gatatgactg
     1441 tgctatctat tcaactttcc ggcttcttga gatagtttca aaaatggacc atgtcgattt
     1501 cgacaaactt cttgaaggga ttcctgaaac attctcaacc cctgagatcc gtcttgaagt
     1561 agatgatctt aaaaaattcg gaattgtaga aaatgtcgtc aaatatttca aaaatgaagg
     1621 tgccgatgtt aatgacattg acggggtcag ggttactttc gatgacggat ggggactgat
     1681 gcgcccgagc aatacacaga atgtcatagt gctgagagtc gaagctgatt cgttgaaaag
     1741 aatgaatgaa ataagagagt tacttgaaag aaaacttgac gagtacaaca gataaccgga
     1801 gcaacacatg tcaaaagcat tagttatgat ccttgccgga gggcagggga aacggctcgc
     1861 accgctcact cttgacagag cgaaacctgc agttcctttc ggaggaacat accggataat
     1921 tgacttcgtt ctgagcaact ttgtcaactc cggttttatg cagatcaaag ttctgactca
     1981 gtttatgagc aactcactga accagcacat ttcaaggaac tggacacttt caccgacatt
     2041 caatcagtat gtcgattctg tccctgcaca gatgagaacc ggtgac
//
LOCUS       DAOOYB010000398         2067 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_68872_length_2067_cov_4.909046, whole genome shotgun sequence.
ACCESSION   DAOOYB010000398 DAOOYB010000000
VERSION     DAOOYB010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68872_length_2067_cov_4.909046"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2067)
                     /locus_tag="PL056_13915"
     CDS             complement(<1..>2067)
                     /locus_tag="PL056_13915"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HPM48157.1"
                     /translation="AVNLISASIYGKKTTFKENLQLYGMAILFLVVLYDSASSLLFYW
                     TFNNIFSLIKNVIYNRIYDKGIIRSAKTIAKKDTFAFDRIFISALISFLLLTFTAAPL
                     SVLSSGSKSDFEEPLFHYMGYSLFFSGILFLLAIILYFNVPYKIKILLSFTTTAAVVL
                     GLLNMFIFTGSYGDMSHFIFSEGIEIDDIDIYLNVISGIVILTALLFLFYRKNFHFIR
                     SFLTVISGSLILFCFYEAACFYNTENSKTESKPGEYSFTFSKTGRNVVIIMLDRFIGG
                     FVPQLLELIPEIKENYDGFVYYSNTLSPASYTIGGVPAIMGGWEYTVRNVNTTRTDVP
                     LIKKLDESARIMPYNFDKAGFDVTIFSNDIYGWFKNDSRENIGDSLFIHPDFPKFRDQ
                     WLERYSKESRKDDYSIRKKLLVFGIFRSAPLFIREFIYDSGEWHVEEEPENSIEEKDE
                     KNYIYFHQKSRHRRDTTLKYYAALDFLPEMSSVSDDPKNKFIYMTNDLTHEPHLIDKN
                     FKFEITGKIGYPRDLYKKFNKNMNSLKHLYTDGAALKLVNEWLNWMKDNGVYDNTRII
                     IVSDHGRAMYDPYFKSQKIPKARKRGHPVDFDNLLMVKDFNSRGNLLINKTFMSSADV
                     PSIAMERIIEGTNPYTGKKIESPFSRFPFYAYDIQWRLEKQEKFRYKIHEGYVIEEND
                     FDNCVI"
BASE COUNT          655 a          371 c          357 g          684 t
ORIGIN      
        1 ctataacaca attatcaaag tcgttttctt ctatcacata accttcgtgt attttatatc
       61 tgaatttctc ctgtttttcc agtctccatt gaatatcata agcataaaaa ggaaatctgc
      121 tgaaagggct ttcgatcttt ttaccagtat aaggatttgt tccttcaata attctttcca
      181 ttgcaattga aggaacatca gctgatgaca taaaggtctt gttaattaaa aggtttccgc
      241 gggaattgaa atctttcacc atcaaaagat tgtcaaaatc aaccggatgc ccccttttcc
      301 ttgctttagg tattttctgg gacttaaaat aagggtcgta cattgctctg ccatgatcgg
      361 aaacgattat tattcttgta ttatcgtaaa ctccgttatc tttcatccag ttaagccact
      421 cgttgacaag tttcagggcc gctccgtctg tgtaaaggtg tttcagtgaa ttcatgtttt
      481 tgttgaattt tttatataga tctctggggt atcctatttt tcctgttatt tcgaatttaa
      541 aattcttgtc aatgagatgc ggttcatgtg taaggtcgtt agtcatataa atgaatttat
      601 ttttcggatc atctgaaaca ctggacattt caggaagaaa atcaagtgca gcataatatt
      661 tcagagttgt gtctctgcga tgccggctct tctgatgaaa atatatataa ttcttttcat
      721 ctttttcttc tattgaattt tcaggctctt cctcaacatg ccattctccg ctgtcataaa
      781 taaattccct tataaaaaga ggtgccgatc tgaaaatgcc gaaaacaagc aacttttttc
      841 taatagaata atcatctttc ctgctttcct tgctatatcg ttcaagccac tggtctctga
      901 atttgggaaa atcaggatga atgaacaaag agtcaccgat attttccctg ctgtcatttt
      961 taaaccatcc gtaaatgtca tttgaaaaaa ttgttacatc aaatccggcc ttatcaaaat
     1021 tatacggcat tatcctcgcg ctttcatcca gtttttttat taatggaaca tctgttcttg
     1081 ttgtgtttac atttctgaca gtatattccc atccacccat tattgcggga actcctccta
     1141 ttgtgtaact tgcgggggaa agggtgttgc tgtaataaac aaaaccgtcg taattttctt
     1201 tgatttcagg aatgagttca agaagttgag gcacaaaacc gccgataaat ctgtcaagca
     1261 tgataataac aacattgcgg cctgtttttg aaaaagtgaa cgaatattca ccgggctttg
     1321 attccgtttt actattttct gtattataaa aacatgctgc ttcataaaaa cagaataaaa
     1381 taagagatcc cgaaataact gtaagaaaag atctgatgaa gtgaaaattt tttctgtaaa
     1441 aaagaaataa gagtgctgtc agaataacta ttccggatat aacatttaaa tatatatcaa
     1501 tgtcatcgat ctctattcct tcactgaaaa taaaatgact catgtcacca taactgcccg
     1561 taaagataaa catatttaac aatcccagaa caacagcggc ggtcgttgtg aatgaaagca
     1621 gaatctttat tttatacgga acattgaaat ataatattat tgccaggagg aagagtatcc
     1681 cggaaaaaaa cagagagtat cccatataat gaaaaagcgg ttcttcaaaa tctgattttg
     1741 atccggacga cagaacagaa agcggagcag ctgtaaaagt gagcagtaaa aaagaaataa
     1801 gtgctgatat gaatatcctg tcaaaagcaa atgtatcttt tttcgctatt gtttttgcgg
     1861 atctaataat tcctttgtca tatatcctgt tgtagataac attcttgata agactgaaaa
     1921 tgttgttaaa tgtccagtaa aagagcagtg agcttgccga atcataaaga acaacaagaa
     1981 aaagaattgc catcccgtaa agctgcaggt tctccttgaa agttgttttt ttgccgtaaa
     2041 ttgatgctga gataagattt acagcag
//
LOCUS       DAOOYB010000399         2048 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_69864_length_2048_cov_2.675364, whole genome shotgun sequence.
ACCESSION   DAOOYB010000399 DAOOYB010000000
VERSION     DAOOYB010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69864_length_2048_cov_2.675364"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="PL056_13920"
     CDS             complement(<1..534)
                     /locus_tag="PL056_13920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48158.1"
                     /translation="MKKALSFLSVLIIMILFSGCTKIPEKTDIEELIGEISDEIANQC
                     GNGITNEGEECDFEYIRSCSSYDPETVGPIKCENCFLDFSNCQLKESCNANKCKSKGE
                     CKAIKYLDYEIYCDCFDNRTGEHCEYCSPYFHFDFDRECISDSICTEIGCELENQECF
                     IDGNSSKCRCKHPWTGEK"
     gene            complement(545..>2048)
                     /locus_tag="PL056_13925"
     CDS             complement(545..>2048)
                     /locus_tag="PL056_13925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48159.1"
                     /translation="TIDFCVNSREIRCEHDPAAPLNSTDIVSFSTITYTDENGWTEPD
                     YCQWICHEGLMKDSGSCINPYIKQYTLNGYPYVAGFDESNNLLVIYVSSMYSVSKTEM
                     KKISEFGSSFTGGIIGNDRNIYLRNKYGFGVYFRNSDEFLKISSSVTDSQNMSLLNSA
                     VFFGNSYLDFPEINYINGEIIDENASSISDKDGNILTVYNNGTVKLNKSDLSIIWKKN
                     YSEYLFSKNPVIDSQENVHIPCRNNSKSCILTIRLSNGSIVGKFEFPDSSEISHQPAI
                     SIGSNLKKYAVQGGVLTIIDKDNNILAETSKVVPKKLITNDYAPVVTDRGLTYFSNYE
                     SVVCVNEAGEQIWIQDTDNIVYSFFHHDNTLYVFNGSGVYLYNAPGNISGDWPHFKHD
                     ARLSNSLLENEFVDPPSAPVALSPEDSTVFNTNSVTFSWAIDSGDPEIRYTLLLRDSA
                     GYDEVYAGPEIGLTTAIVSDLAAGSYEWRVVSKDKNGSLNVSDARTFEIE"
BASE COUNT          613 a          400 c          359 g          676 t
ORIGIN      
        1 tttttcacct gtccatgggt gtttgcatct gcatttagag ctgttcccat ctatgaaaca
       61 ttcctgattt tccaattcac atcctatttc agtgcaaatc gaatcagaaa tacattctct
      121 gtcaaaatcg aaatgaaaat acggagaaca atactcacaa tgttcaccag ttctattgtc
      181 aaagcagtca caataaatct catagtccaa gtatttaatt gctttgcatt ccccttttga
      241 tttgcatttg ttggcattgc aggattcctt taattgacag ttgctaaaat ccagaaaaca
      301 gttttcgcat tttatcggcc ctacagtttc aggatcataa cttgaacatg atctaatgta
      361 ttcaaaatca cattcttctc cttcatttgt tataccgtta ccgcattgat ttgctatttc
      421 atcagaaatt tctcctatca gttcctcaat atcggttttc tcagggattt ttgtgcatcc
      481 tgaaaaaagg atcatgatta ttaagactga aagaaaagag agagcttttt tcattatttc
      541 accttcattc aatttcaaaa gtccgtgcat ctgacacatt aagtgacccg tttttatctt
      601 ttgaaacgac acgccattca taacttcctg cggcaagatc actgactatt gcagttgtca
      661 gtccgatttc tggaccagca taaacttcat cataaccggc tgaatctctt aacagaagtg
      721 tgtatcttat ttctgggtcg ccagaatcaa tagcccacga aaaagtgaca gagtttgtgt
      781 taaaaacagt gctgtcttca ggagataaag caaccggagc agatggcgga tcaacgaatt
      841 cgttttcaag gagtgaattt gaaagtctcg catcgtgctt aaaatgaggc cagtcaccgg
      901 aaatgttgcc gggagcgtta tataagtaaa ctccgcttcc attaaacaca taaagggtat
      961 tgtcatggtg aaaaaagctg taaactatat tgtctgtatc ctggatccag atctgttctc
     1021 ctgcctcatt gacacacacg acactttcat aattactgaa ataagtcaag ccgcggtcag
     1081 tcactacagg cgcataatca tttgttatta atttttttgg aacaaccttt gatgtttcag
     1141 ctaatatgtt gttatctttg tcgataattg ttagtacgcc accttgaacc gcatattttt
     1201 tcaaattact gccgatagag atagccggtt gatgagatat ttcagaagaa tcggggaatt
     1261 caaattttcc aacaatactg ccatttgaaa gtctgatcgt aagtatgcat gatttcgaat
     1321 tgtttctgca tggaatatga acattttcct gactgtcaat gacaggattt ttggaaaaca
     1381 ggtattcaga ataattcttt ttccatatta ttgaaaggtc acttttattt aatttcactg
     1441 tgccgttatt gtaaacagtc agaatgtttc cgtccttatc agatatacta gatgcgtttt
     1501 catcaatgat ttcaccattt atataattta tctcgggaaa gtcgaggtat gaattaccaa
     1561 aaaaaacagc agagtttaaa agcgacatat tttgtgaatc agtgacagag ctacttatct
     1621 ttaaaaactc gtcactgttt ctgaaataaa caccaaaccc gtatttgttc cttaaatata
     1681 tattccggtc atttccaata attccgcctg tgaaagatga accgaattca gagatctttt
     1741 tcatttccgt ctttgagacg gagtacatgc tcgaaacata aattaccagc aaattattgc
     1801 tttcatcaaa accggcaaca taaggatatc cgtttaaagt atattgtttt atgtaaggat
     1861 ttatacatga accggaatct ttcattaacc cttcatgaca gatccattgg caatagtccg
     1921 gttcagtcca gccgttttcg tcggtgtagg taattgttga aaaagaaacg atgtctgtcg
     1981 aatttaaagg tgcggcaggg tcgtgttcgc atctgatttc tctcgaattt acacagaaat
     2041 cgatggtg
//
LOCUS       DAOOYB010000400         2043 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_70062_length_2043_cov_3.720825, whole genome shotgun sequence.
ACCESSION   DAOOYB010000400 DAOOYB010000000
VERSION     DAOOYB010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70062_length_2043_cov_3.720825"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1996)
                     /locus_tag="PL056_13930"
     CDS             complement(<1..1996)
                     /locus_tag="PL056_13930"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP and VCBS repeat-containing protein"
                     /protein_id="HPM48160.1"
                     /translation="MLKWSTVTDDHFNTSVSGIYDVEITNDVPSIAVKKSYGYAIGAG
                     PVIDGYSELSPAWMLSEDLNCTGNDCLTEIEVWTDAPAGSTVYISVNGGAETSKVTAA
                     SSGMSYADFGIILDSSVLHNTLNVRVVSTGTNEETYYIDIDEEIPDLALINPVDCSAG
                     DVCRRLDLIDNPATTDYIEMAELGYGYNDDAVTGGALNFKSSGVIIFEVTGATAGMVK
                     VESVTGGEIAGITNGEAPVLYDGIEGIYYADFSNMTVVDDNAKGQTDYDVVFKVTESP
                     SGAVSKYLVKLHVDLLKPAAINIDGTLSVNKKTAEVAVDWTAVEGNDSVLGGVSGAVH
                     EYDIRYQDYNEGTCTIATAFEGAKKPLETIAGTVPDPIITGDMTYNFFVNRINNGQTG
                     INLKEADIHRNGNKYCFAVAAVDAVYASDGTVLARNTGTISPNDIGEVKMAWSEIEPD
                     SFGFHFNVIRNLGDLDGDGLDDFVIADSYRSGDGTTNNYFGRIEIYYSKGDASFVVSG
                     SENMEVLGAGISSKTDFNKDGHLDFAYTNAFGEIFIHYGTTEGLTSIPSYTFVSKDES
                     NQTARTMATGDYNGDGCDDIAVSAPTFNGAGSARGQVYIYFGRGTECSSNDPMDGEAP
                     DVVFEGSANNDRLGRGEIYSVGDINGDGKTDFAFSTDNKIF"
BASE COUNT          564 a          487 c          379 g          613 t
ORIGIN      
        1 taaagatttt gttgtctgtg gaaaacgcaa aatcagtttt tccgtcacca ttaatatctc
       61 cgacagaata aatttcacct cttccaagac gatcattatt ggcacttcct tcaaaaacaa
      121 catcaggggc ttctccatcc atcgggtcat tggaagaaca ttcggttcct cttccgaaat
      181 aaatgtaaac ctgacctctt gcactccctg caccattaaa tgttggagct gacacagcta
      241 tatcatcaca cccatcccca ttataatcgc ctgttgccat ggttcttgcg gtttgattgc
      301 tttcatcttt tgaaacaaaa gtataggaag gaatagatgt taaaccttct gtagttccat
      361 aatgaataaa aatttcgcca aaggcattgg tatatgcaaa atcaaggtgt ccatctttgt
      421 taaaatcagt tttactggaa ataccagctc cgagaacctc catattttca gatcctgaaa
      481 caacaaaact cgcatctcct tttgaatagt atatctctat tcttccaaag taattatttg
      541 ttgtgccatc tccgcttctg tacgaatcag caataacaaa atcatcaagc ccatcaccgt
      601 caagatcgcc caagtttctt atcacattaa aatggaaacc aaaagaatca ggttcgatct
      661 cactccacgc cattttcact tcgccgatgt cattggggga gatggttccg gtgtttctgg
      721 ctaatactgt gccgtcgctg gcataaactg catcaactgc cgcaactgca aaacagtatt
      781 tgttgccgtt tctgtggata tctgcttctt tgagattaat cccggtctga ccattgttga
      841 tcctgttgac aaagaaatta tatgtcatgt cgcccgttat gatcggatcg ggaacggttc
      901 ccgcaatggt ttcaagcggt ttttttgctc cttcaaacgc agttgctatc gtacatgttc
      961 cttcgttata atcctgataa cggatgtcat attcatggac agcgccggac actccgccca
     1021 atacagagtc gttcccttca accgcagtcc agtcaacagc aacttccgca gtctttttgt
     1081 tgaccgaaag agttccgtca atatttattg cagccggctt aagcagatcg acatgaagtt
     1141 tcacaagata ttttgaaact gcacctgatg gcgattctgt gactttgaat acaacatcat
     1201 agtcagtctg accttttgca ttgtcatcaa cgaccgtcat atttgagaaa tcagcataat
     1261 aaattccttc gatgccgtca tagaggactg gagcttcacc atttgttatt cctgcaattt
     1321 ctccgcctgt aactgattca acttttacca ttccggcagt tgctcccgtc acttcaaaaa
     1381 ttatcacacc tgatgactta aaattcaacg ctccgcctgt tacggcatca tcattataac
     1441 cgtaaccaag ttcagccatt tcaatataat ctgtcgtggc agggttgtca atcagatcaa
     1501 gccgtctgca gacatcaccc gctgaacagt caacaggatt tataagcgcg agatcgggga
     1561 tttcttcatc aatatcgatg tagtaagttt cttcgttagt tccggttgat acgactctga
     1621 catttaatgt gttgtgaaga acagaactgt caagaattat gccgaaatcg gcataggaca
     1681 ttcctgaaga tgcggctgtt acttttgaag tttcagctcc tccgttaact gaaatataga
     1741 cagtcgatcc cgccggagca tctgtccaga cctcaatttc tgtcagacag tcatttcctg
     1801 tgcagttgag atcttccgaa agcatccatg cgggggacaa ctcggaataa ccgtctatga
     1861 ccggaccggc accgatggcg tatccataac tcttttttac tgcgatcgat ggaacatcgt
     1921 ttgttatctc aacatcataa attcctgaaa cagaagtgtt gaagtggtca tctgtaacag
     1981 ttgaccactt caacacttct gtttcaggaa tttatgatga gagataacaa acgatgttcc
     2041 atc
//
LOCUS       DAOOYB010000401         2027 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_70867_length_2027_cov_5.972110, whole genome shotgun sequence.
ACCESSION   DAOOYB010000401 DAOOYB010000000
VERSION     DAOOYB010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70867_length_2027_cov_5.972110"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..333
                     /locus_tag="PL056_13935"
     CDS             58..333
                     /locus_tag="PL056_13935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48161.1"
                     /translation="MRFSKKQRARIDLVNEHLKKGVLITHTCDGGMLAEHYYEGTDKS
                     GNIVGIPTPETKRFMGRFLSSISIHPLNITHINRQSVESIPYLASGL"
     gene            613..1266
                     /locus_tag="PL056_13940"
     CDS             613..1266
                     /locus_tag="PL056_13940"
                     /inference="COORDINATES: protein motif:HMM:NF018691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPM48162.1"
                     /translation="MGVIISIANKKGGVGKSTIATNLACFFSSSGKSVVLIDADEQKS
                     SMYFQELRPDTAKQFQTVSITTRTILREAEKFNADYVIIDVPAKDSPVSRAAIAAADR
                     VIVPVQPSQYDILASEDTFRLLDEVAAQKQNFKFAVIQNMVLSNPKVKITGEVSEVLK
                     DMAKEFGLHLFNSVLYMRIAYKDSAEKGLSVAEMKGEKYTKAEEEFNSFYKEVLKWL"
     gene            1257..1517
                     /locus_tag="PL056_13945"
     CDS             1257..1517
                     /locus_tag="PL056_13945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48163.1"
                     /translation="MVVRKSDIPKNNKEKLSLDKFVNSSPDEVTTSKDMLTRESFTFR
                     VNRDLWDDFDYWCKKNRVSRSDVLENFLLEKLGRKKEDYRTK"
     gene            1670..>2027
                     /locus_tag="PL056_13950"
     CDS             1670..>2027
                     /locus_tag="PL056_13950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48164.1"
                     /translation="MSNEKMNDLKKAVKEKEESELSSKMERNDIQLKLFDEEKIPFYS
                     QDLIEKKSVFLPNTSRATSFEIARSNSVKITIGRAGGHKDYGVLKTKHRDVLYAVLAI
                     WASQNWPTWKNKDGEFL"
BASE COUNT          678 a          369 c          442 g          538 t
ORIGIN      
        1 aaattggagc ttgccgatga aaacagttaa caagcttttt taaggagggg aacaaatatg
       61 agattttcaa aaaaacagag agccagaatt gatcttgtca atgagcacct taaaaaaggt
      121 gttctgataa ctcatacctg tgacggcgga atgcttgcgg aacattatta cgaaggaaca
      181 gacaagtccg ggaacattgt cggtattccc acaccggaaa caaagagatt tatggggaga
      241 ttcctttcaa gcatttcaat tcatccgctg aacatcaccc acattaaccg acagtccgtt
      301 gaatcaatac cttatttagc ttccggtctt taaaaaaaag aatcccaatc aaaacagaag
      361 gttagaatct cggttttccg ctggtgacag cttcgcttcc accgctctaa acagtcctgc
      421 tgaacgcagg acaaacttga ttggattgga ctttagtgaa aaacgaatac cgcttgaaac
      481 ggcttttaag cataccgccc ctttaaaata attcaatttt ttcttgactt cataactttg
      541 taaagttata aagctatagt tttttaaatt tgcagtgtta ctgttttatg attttaagaa
      601 gtgaggcggc ttatgggagt aattatttca attgcgaaca agaagggcgg agtaggtaaa
      661 agcaccattg cgaccaatct tgcatgtttt ttcagttcgt ccgggaagtc ggttgttctg
      721 atcgatgcag acgagcagaa atcaagtatg tattttcagg aactgcgacc cgacacggca
      781 aaacagtttc agacagtttc aatcacgaca agaacaattt taagagaagc cgagaagttt
      841 aatgcggact atgttatcat tgatgttccg gcaaaggaca gccccgtatc gagagcggca
      901 attgccgccg ccgaccgtgt aattgttcct gttcagccga gtcagtatga catactggca
      961 agcgaggaca ctttccggct tcttgatgaa gtggccgcac agaaacagaa tttcaaattt
     1021 gccgtaattc agaacatggt actttcaaat ccgaaagtaa aaattaccgg agaagtttca
     1081 gaagttctga aggacatggc gaaagaattc ggactgcatc tttttaattc ggttctttat
     1141 atgaggatag catataagga cagtgccgaa aagggactga gtgttgctga aatgaaaggg
     1201 gagaaataca caaaagccga agaggagttt aattcatttt ataaagaggt tttaaaatgg
     1261 ttgtaagaaa aagcgatatt ccgaagaaca acaaggaaaa attatcactt gataaattcg
     1321 taaattcatc acctgatgaa gttacaactt caaaggatat gctcacaaga gaatcgttca
     1381 ccttcagggt gaaccgtgac ttgtgggatg attttgatta ctggtgtaaa aagaacagag
     1441 tttcaagaag cgatgttctt gaaaacttcc tgcttgagaa attaggcaga aaaaaagaag
     1501 attacagaac caaatgacaa agttttcttt ttcccgctat taagtagaat tatagcgggg
     1561 gatagataaa aggtagctcc ttttgttgaa cggcggtatg aagggacggc cttccccctt
     1621 tgttttataa cccttctttt gaaccctaac ccttcaagga ggatttcaga tgtcaaatga
     1681 aaagatgaac gatcttaaaa aggcagttaa agaaaaagag gaaagcgaac tttcttcaaa
     1741 aatggagcgg aacgacattc agcttaaact ttttgatgaa gaaaagatcc ctttctacag
     1801 tcaggacctt attgaaaaaa aatcggtttt ccttccgaac acatcaagag ccacatcttt
     1861 tgaaattgca agaagcaact ctgttaaaat aacaatagga agggccggag gtcacaaaga
     1921 ttacggagtt ttaaaaacca agcatagaga tgttctgtat gcagttcttg caatatgggc
     1981 aagccagaac tggccgacat ggaaaaacaa ggacggtgag tttctcg
//
LOCUS       DAOOYB010000402         2020 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_71205_length_2020_cov_5.691603, whole genome shotgun sequence.
ACCESSION   DAOOYB010000402 DAOOYB010000000
VERSION     DAOOYB010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71205_length_2020_cov_5.691603"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            142..978
                     /locus_tag="PL056_13955"
     CDS             142..978
                     /locus_tag="PL056_13955"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HPM48165.1"
                     /translation="MLNWLSYGVVDISKYTTPLIFAVAITSWFLLLKKYIPVKTNYRL
                     LIKVAAASCVGAGLSPLLIIIFSGKFVSFLSSSMTKIQKINDWNAFQALLAVLFKGFS
                     ITGGLLILTIVLLFVVHDARKLIFGIFYPFPLFAAITRINCFMEGCCFGKISEGPCAI
                     KYPPASHASKLHHLKHGLISRFEVSYPVHPVQAYLVISMLLLFIILFIMNRFNVRRNI
                     IAGTMLAGYGMINFNMEFLRVEPLLYNFLTVGQIMEILITALGFYIIFKVRDCDIKEN
                     LK"
     gene            975..1325
                     /locus_tag="PL056_13960"
     CDS             975..1325
                     /locus_tag="PL056_13960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48166.1"
                     /translation="MKKFIAVLIIISVIIIGYRTYNYFERKNVIDSKIPKETTVIDDV
                     KGIIGIEKEKEEPQYSSEQIRVFVPEKKNDSVKTYTNQDIKKLNIRKFKLKKFITEKD
                     LEKYKEKPGNSDTK"
     gene            1416..>2020
                     /locus_tag="PL056_13965"
     CDS             1416..>2020
                     /locus_tag="PL056_13965"
                     /inference="COORDINATES: protein motif:HMM:NF019194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 family protein"
                     /protein_id="HPM48167.1"
                     /translation="MPNVCKKKIKISFDQAILLIVPYCKTRLMEQIRSVLPVILYLIF
                     FQTVILDLPLADSSIISFGMALVILGLAFFMEGLLLGIMPLGEIIGFKLPIKSGIFTI
                     VIFSFILGVGATLAEPSISVLKAAGSFVKPWDAPLLFLLLNRHAGLLVAALGIGVGIA
                     VIAGMLRFMYSISLKPFLYTLIPFLLIISIWGIFDKNILYLT"
BASE COUNT          613 a          404 c          363 g          640 t
ORIGIN      
        1 ttaagctgga tatatctgac cgtcggcgga gaaaatctga taagcggtga aagagatctc
       61 tttttcggtt tcgggctcag attcactgat aacgatctga aaacgatcct cacatcagtt
      121 cctatgccgt gaggtgaatg aatgcttaac tggctttcat acggtgtggt tgacatctca
      181 aaatatacaa caccgctgat atttgcagtt gcaataacat catggttcct tcttctaaag
      241 aaatatattc ctgtaaaaac aaattaccgt cttctcataa aagtagctgc cgcttcatgt
      301 gtcggtgcag gattgtcgcc tcttctcata attatatttt ccggaaaatt tgtctctttt
      361 ctttcctctt cgatgacaaa gatccaaaag ataaatgact ggaatgcatt tcaggcactt
      421 cttgctgtac tttttaaagg attttcgata acaggcggac ttcttattct tacaatagtc
      481 cttctgtttg ttgtacatga tgccagaaag ctcattttcg gaatatttta tccgttccct
      541 ctttttgcgg caatcaccag aatcaactgt ttcatggaag gttgctgttt tggaaagatc
      601 tcggaaggtc catgtgcaat aaaatatcca cctgcaagtc acgcttcaaa acttcatcac
      661 cttaaacacg gacttatttc aagatttgaa gtcagctatc cggttcatcc ggtacaggca
      721 tatctcgtaa tatcaatgct actccttttt ataattctct ttattatgaa ccggtttaat
      781 gtccgcagga acatcatagc aggaacaatg ctcgcaggat acggaatgat aaatttcaac
      841 atggaattct taagggtaga accccttctc tacaactttc ttaccgtagg acagataatg
      901 gagatactta taactgctct tggattctat ataatattta aagtcaggga ttgtgacata
      961 aaggaaaact taaaatgaag aaattcattg ctgttctaat aattatttct gtaattataa
     1021 taggttacag gacatacaac tattttgaga gaaaaaatgt cattgacagt aaaataccga
     1081 aggaaactac tgttattgat gatgtgaaag gtataatcgg tattgaaaaa gaaaaagaag
     1141 agccccaata cagttccgaa cagataaggg tatttgttcc agaaaagaaa aatgattctg
     1201 taaaaacata taccaaccag gatataaaaa agctcaatat cagaaaattc aaactgaaaa
     1261 aattcataac tgaaaaagat ctcgagaaat acaaggaaaa acctggaaat tcagacacaa
     1321 agtagttgcc gtttatctta ttcaaaaatt tgatatttat gaacattccg ttatcattac
     1381 ctgtttagtt aattatcacc tttccggaga gctccatgcc aaatgtctgc aagaaaaaaa
     1441 tcaaaatatc tttcgatcag gcaatacttt tgatcgttcc atattgcaaa acacgcctca
     1501 tggaacagat caggtctgtg cttcctgtaa tactttacct catctttttc cagacagtga
     1561 tactggacct gcctcttgcc gattcatcca taatttcatt tggaatggcc cttgtcatcc
     1621 tgggactcgc ttttttcatg gaaggactgc ttcttggaat aatgcctctt ggagaaataa
     1681 tcggattcaa actgccgata aaatcaggta ttttcacaat agttatattc agttttattc
     1741 tcggtgtggg agcaactctt gctgagccgt cgatatcagt tcttaaagcg gcaggatctt
     1801 tcgtaaaacc gtgggatgct ccattacttt ttcttctgct caacaggcat gccggtcttc
     1861 ttgtggcagc tctcggtatc ggtgtcggca tcgcagtcat tgcaggaatg ctccgcttca
     1921 tgtattcaat atcgctcaaa ccctttctct atacgctgat cccgtttctt ctgatcattt
     1981 caatctgggg gatctttgat aaaaacatcc tttatcttac
//
LOCUS       DAOOYB010000403         1942 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_75533_length_1942_cov_3.374669, whole genome shotgun sequence.
ACCESSION   DAOOYB010000403 DAOOYB010000000
VERSION     DAOOYB010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75533_length_1942_cov_3.374669"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..>1942)
                     /locus_tag="PL056_13970"
     CDS             complement(62..>1942)
                     /locus_tag="PL056_13970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48168.1"
                     /translation="DKGFISCMDNEGEYYSPVKSGIYSLSGWSASDIYAAGRAGILHY
                     DGTAWTVEFETATKMMSVMVSQNSVFAGGENLLAVKKDVWKTLSISGNVESIAVIDDT
                     SAAALVKKDGNPSKIIIFDENPAIISETELEDADYTALSYYKGSIVAGGKNGVMNIDG
                     TVINFDLPWDINGLKVHNDVLQIFGTSGFLASWDEEKLSSNKGFFKNKASAVFATSPS
                     SIFISAGTMIIQILPNSVNYFPLTAEIEDVFGISENEIYVKTKEGLFKYNGFAIRKVK
                     DPDAVDTTGMISERNKFMTKTADSVTWKFNEKLTFEYSCITESCFVTLADIDGMLNKI
                     RTFPNDGITTAPVGARFTSDGFIAAGGDSVYTIKDGMTDSKYYDFEITSIDAGNASII
                     LGTSEGSVHVVKNDTIENSFDLGTGSPVTSVSINDSGHIATLTGGFIYLKTDSDFINI
                     PVLSGKKINSVWLFDDDSFIASGEDIVLEYDGNMMVANLPKDLIDRGLPTGETEVNMH
                     RIFALDPDNIWILGSSPDGKTAYIFSFDGIKWINDARSSLYDEFSFTDVWACSQDKVL
                     ISMSSEGWIYHYKNMTTQGFIESVDNPLGEPIYSIAADPESDLYIFGENGIVLRADSP
                     CI"
BASE COUNT          554 a          421 c          356 g          611 t
ORIGIN      
        1 tgtaaaaaca cacactgctg aatggaatta tataaagagt tgatgaaata tcaaggagat
       61 gttaaataca gggactatct gctctcaaaa ctattccgtt ttcaccgaaa atgtaaagat
      121 cgctctctgg atctgccgca attgaataga tgggctcgcc gagaggattg tcaacgcttt
      181 cgatgaaacc ctgagttgtc atgttcttgt aatgatagat ccagccctca ctgctcatag
      241 agataagaac cttatcctga ctgcatgccc agacatctgt aaagctaaac tcgtcataaa
      301 gagaacttct tgcgtcattt atccatttta ttccgtcaaa gctgaaaata taggcggttt
      361 tgccatccgg tgaactgccg agtatccaga tgttgtcagg gtcaagtgca aagatcctgt
      421 gcatgttcac ttcggtttct cctgtcggta gtccgcggtc gatcaggtcc ttaggaagat
      481 ttgcgaccat catgttcccg tcatactcca aaacgatatc ttctcccgat gcaataaatg
      541 agtcgtcatc aaaaagccat acagaattga tttttttacc cgaaagaaca ggtatgttta
      601 taaaatcgct atcagttttt aagtaaataa atccgccggt caatgtggca atatgtcctg
      661 aatcattgat cgagacgctt gtcacgggtg aacccgtccc gaggtcgaaa ctgttttcaa
      721 tagtgtcatt tttgacaaca tgaactgatc cttcacttgt tccaaggata atagacgcgt
      781 tgcctgcatc aattgaagtt atctcaaaat cgtaatattt agaatccgtc ataccgtcct
      841 taattgtata gacagaatct cctccggctg caataaaacc gtctgaggta aatctggctc
      901 cgacgggagc ggttgtaatt ccgtcatttg gaaatgtcct gattttgttt aacatcccat
      961 caatatccgc gagtgtcaca aaacagctct ccgtaatgca actgtattca aaagtaagtt
     1021 tctcattaaa tttccatgtg acagaatcag ctgtttttgt catgaatttg ttcctttccg
     1081 atatcattcc ggttgtatcg acagcatcgg gatctttaac ttttcttatt gcaaaaccgt
     1141 tatatttaaa aagaccttcc ttggttttga catatatttc gttttcacta attccaaaaa
     1201 catcttctat ttcagcagta agtggaaaat aattcactga attcggaagt atctgaatta
     1261 tcattgttcc tgccgaaata aatatgctgg aaggggaagt cgcaaaaacg gcgctcgcct
     1321 tgtttttaaa aaatcctttg tttgaagaaa gtttttcttc atcccatgaa gccagaaatc
     1381 ctgaagtgcc gaatatttga agaacatcgt tatgaacctt tagtccgtta atatcccacg
     1441 gcagatcaaa attaatcaca gttccatcaa tattcattac accgtttttt ccgcctgcga
     1501 cgatactgcc tttgtaatac gacaaagcag tataatcggc atcttcaagt tcagtttctg
     1561 atattattgc agggttttca tcaaaaatta tgatctttga aggatttccg tcttttttta
     1621 caagtgcggc ggctgatgtg tcgtcaatta ccgcaataga ttcaacattc cctgaaatag
     1681 agagggtttt ccagacatct ttttttaccg caagaagatt ttccccacct gcaaaaacgc
     1741 tgttttgcga aaccattacc gacatcattt ttgttgccgt ttcaaattcg actgtccacg
     1801 cagttccgtc ataatggagt atcccggctc ttcctgccgc ataaatatct gacgcgctcc
     1861 atcctgataa tgaatatatt cctgatttga ctggagaata atattcgcct tcattatcca
     1921 tacagcttat gaaacctttg tc
//
LOCUS       DAOOYB010000404         1938 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_75740_length_1938_cov_3.913967, whole genome shotgun sequence.
ACCESSION   DAOOYB010000404 DAOOYB010000000
VERSION     DAOOYB010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75740_length_1938_cov_3.913967"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..153)
                     /locus_tag="PL056_13975"
     CDS             complement(<1..153)
                     /locus_tag="PL056_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012424898.1"
                     /note="catalyzes the interconversion of 2-phosphoglycerate
                     and 3-phosphoglycerate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HPM48169.1"
                     /translation="MILDGFGIRESSNFNAVKIAKKPNIDYLFENFPKTELDGSGLAV
                     GLPRGQM"
     gene            complement(190..1581)
                     /locus_tag="PL056_13980"
     CDS             complement(190..1581)
                     /locus_tag="PL056_13980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48170.1"
                     /translation="MKRFFATLVLLMFIFTTGCDDSKTKKPANDSNNDTSDTDSVTDA
                     ENGDAETPDGEETGDCGNNIVETGEICDGGLKNCTEIDPKLYISGKAKCKSDCSGWDT
                     VTCEETDAVCGNETVEVPEVCDGNIENCVDIDPAKFKSGKAKCNRDCDGFDTVTCEEL
                     DDYCGDGKVSETEMCDGDLKSCIDIDSKIYSGGTAECKDDCSGYDVTDCVEIVAVCGD
                     ETVEGKEVCEKGDLKNCVEINSTLYKGGKAYCKNDCLGWDVITCEDKGNILFSDDFEN
                     GDSKWTLTGDWEIGYPYFDISGVTVNVAVSGDQVLATKLTDGYTVKVNSIASIKDPVT
                     IPATGNFILTFSAYVNTDFDSGTSGISWFDGMIVTFVSGIDAPVELPPSTDNADLIGT
                     FKGLTDGVNYTTFAGIRGTSVNNTYSKFSFDLSPAAGKTVTIEFKFYSDTIQGGSGSF
                     PGIYIDDVVIAEE"
     gene            complement(1679..>1938)
                     /locus_tag="PL056_13985"
     CDS             complement(1679..>1938)
                     /locus_tag="PL056_13985"
                     /inference="COORDINATES: protein motif:HMM:NF016503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48171.1"
                     /translation="TYDEGTIDRKVFKPGDWAHLKFRRSKGYCIREEGWMLEYTSGAI
                     PTMLPMGIWDNLFSNLDIKSVVQMFWQYGGMVIKNLLRGRF"
BASE COUNT          515 a          478 c          333 g          612 t
ORIGIN      
        1 catctgccct ctcggaagac cgacagcaag ccccgaaccg tcaagctcgg tttttggaaa
       61 attttcaaac agataatcaa tattcggttt ttttgcaatt ttcactgcat tgaaattact
      121 gctttccctg atcccgaaac catcaaggat catcagtatc acttttctgc tttcagacac
      181 attgcctcct tattcttccg caattacaac atcatcaata tatattccag ggaaacttcc
      241 gcttcccccc tgaatggtat cagaataaaa tttaaactct attgtcactg tctttcctgc
      301 ggccggagag agatcaaagg aaaattttga atatgtgtta ttcacacttg ttccacgaat
      361 accggcgaag gttgtataat ttactccgtc tgtgagtcct ttgaaagtgc ctatcagatc
      421 tgcattatct gtacttggcg gaagttccac cggtgcatca atacctgaaa cgaatgtaac
      481 gatcattccg tcaaaccagc ttatgcctga tgttccactg tcaaaatcgg tattcacata
      541 tgcagaaaaa gtcaggataa aattacctgt tgcggggata gttaccggat cttttattga
      601 agcaatgcta ttcactttga ctgtatatcc gtcagtcagt ttggttgcaa ggacttgatc
      661 tccgcttact gcaacattaa ccgtcactcc cgaaatatca aaataaggat agcctatctc
      721 ccagtctccg gtaagagtcc attttgagtc accgttctca aaatcatctg aaaaaagaat
      781 atttcctttg tcttcacagg ttataacatc ccagcctaag caatcatttt tgcaatatgc
      841 cttgccgcct ttgtacaggg ttgagtttat ttcaacacag tttttcagat cgcctttttc
      901 gcaaacttcc tttccttcga ccgtttcatc tccgcagaca gcaacgatct cgacacaatc
      961 tgtaacatcg tatcctgagc agtcatcttt gcattctgct gttccaccag aatatatttt
     1021 gctgtcaata tcaatgcacg atttaagatc accgtcacac atttccgttt ctgacacctt
     1081 tccatcaccg cagtagtcgt caagctcttc gcatgtcaca gtgtcaaatc cgtcgcagtc
     1141 acggttacat tttgcctttc cgcttttaaa tttagcagga tctatgtcaa cacagttctc
     1201 gatatttccg tcacagactt caggaacttc aaccgtctca tttccgcaga ctgcatcggt
     1261 ctcttcgcaa gtaacagtat cccagcctga acagtcgctt ttacattttg ctttgcctga
     1321 aatatagagt ttgggatcta tttctgtaca gtttttgagt ccgccgtcac atatttcgcc
     1381 agtttctaca atgttgtttc cgcagtcacc tgtttcttca ccgtcaggcg tttcggcatc
     1441 accattttct gcatctgtca cagaatcggt atctgaagta tcgttatttg agtcatttgc
     1501 cggttttttg gtttttgaat catcgcaccc tgttgtgaaa ataaacatta ataaaacaag
     1561 tgttgcaaaa aatcttttca tagactccct ccatctgcaa attaaagcgg taacatgata
     1621 cggactatgc tcataaatgt caaaattacg gtttaatatg aaaagagaga caacagggtc
     1681 agaatcttcc tcttaacaga ttttttataa ccatgccgcc gtactgccag aacatctgga
     1741 cgacactttt aatatcaaga ttgctgaaaa gattgtccca gatccccatc ggaagcattg
     1801 taggtattgc acccgatgtg tattcaagca tccatccttc ttcacggatg cagtaaccct
     1861 ttgatcttct gaacttcaga tgtgcccagt cacccggctt gaaaaccttg cggtcaattg
     1921 tcccttcatc gtaagtcc
//
LOCUS       DAOOYB010000405         1911 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_77282_length_1911_cov_8.782328, whole genome shotgun sequence.
ACCESSION   DAOOYB010000405 DAOOYB010000000
VERSION     DAOOYB010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77282_length_1911_cov_8.782328"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1039
                     /locus_tag="PL056_13990"
     CDS             <1..1039
                     /locus_tag="PL056_13990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48172.1"
                     /translation="YFNSSAVTQNGVFGVTVQSWDYFYPESDCYDSETDCVAKGDGYS
                     IYNSTVLVYDVSGEGQYESVPVKITENSRTLDYDNKIIANGTTFWYTDCERKGVDEKD
                     RELLYCYAVPVDVKDPAKPVKGKKVNIPGKIIGMSDDGKYLYTYHYMWNDEVSKDDDY
                     YYNSTYFQTLYILKFNDDKTKVSVVKKISVTNEYESTESMYRYTWSDFIVKNDKVFIG
                     TNETLYNYEQQECNYWYYSNRETSMNVQIFRAEDGKRLFNKSYENGARYSSVEGGGFL
                     LSIEENEDYYYGYTNSNNMIYLSADGVEKELKLAEKTYGYYSYSLSAAVIGDDLFVAR
                     DWNGIEQVSLK"
     gene            1055..>1911
                     /locus_tag="PL056_13995"
     CDS             1055..>1911
                     /locus_tag="PL056_13995"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HPM48173.1"
                     /translation="MSETIKPKLTLFYFSGSGNTKYVAEKFSLQFLEKYGVELVDIDE
                     FDRLRKGFGDDFAVAGVIYPVHALNAPANVLNFLKKSLPKGEGRKFFIVKSPGDPFFN
                     GGATTEIRKILNKNGYIVTHESLVVMPANVFMRYRDSFISKLIEVADVKIAKASSEIM
                     NGTVVLHKNPLWMRFSTWFFSRLETFGASFFGKDLKVNNDCTKCGLCMKICPKMNITA
                     DKEGRPRFGWKCMICMRCIYKCPNNAISPRIMKFFKVKNFYKLSELQKPSDLTNPTAF
                     EKGFGKYFG"
BASE COUNT          625 a          380 c          412 g          494 t
ORIGIN      
        1 ttactttaac agcagcgccg tgacacaaaa tggagttttc ggagtaactg tacagagttg
       61 ggattatttc tatcctgaaa gtgactgcta cgattcagag accgactgtg tcgccaaagg
      121 agatggttac agcatttata attcgactgt tttggtatat gatgtcagcg gcgaaggtca
      181 atacgaatct gtgcctgtca aaataacgga aaacagcaga actcttgatt acgacaataa
      241 gatcatcgcg aacggaacaa ctttctggta caccgactgt gaaaggaaag gtgttgatga
      301 aaaagaccgt gaacttcttt actgctatgc tgttcctgtt gatgtgaaag atcctgcaaa
      361 gccagttaaa gggaaaaagg tcaacatccc cggcaagatc atcggaatga gcgatgacgg
      421 aaaatatctc tatacctatc actacatgtg gaatgatgaa gtttcaaaag atgatgatta
      481 ctactataac tcaacatatt tccagaccct ttacattctg aagttcaatg atgacaagac
      541 aaaagtcagt gttgtcaaga agataagtgt tacaaatgaa tacgagagca cagaaagcat
      601 gtaccgttac acatggtctg atttcattgt caagaatgat aaagttttca tcggaactaa
      661 cgaaactctc tataattacg aacagcagga gtgcaactac tggtattaca gtaatcgtga
      721 aacatcaatg aatgtccaga tcttcagggc tgaggacgga aaacggctct tcaacaagtc
      781 ctatgaaaac ggtgcaagat acagctctgt tgaaggtggc ggattccttc tttcgatcga
      841 agaaaatgaa gattattact acggttacac caactctaac aacatgatct atctgtctgc
      901 tgacggagtt gaaaaagagc ttaaacttgc agaaaaaaca tacggttact attcctacag
      961 cttaagtgcc gcagttatag gcgacgacct tttcgtggca agggactgga acggtatcga
     1021 gcaggtttca cttaaataat catcagttat aaaattgtca gaaactatca aaccgaaact
     1081 cactcttttc tacttttccg gaagcggaaa cacaaaatat gtcgctgaaa agttctcatt
     1141 gcaattcttg gaaaaatacg gtgttgaact tgttgacatt gatgaatttg accggctgag
     1201 gaaggggttt ggtgatgatt ttgccgttgc gggggtaatt tatccggttc atgccctgaa
     1261 cgcgcctgcc aatgttttga atttccttaa aaaatcgttg ccgaaaggtg aggggagaaa
     1321 atttttcatc gtgaaaagtc cgggagaccc tttctttaac ggcggagcaa caacggaaat
     1381 caggaaaata ctaaataaga acggatacat agtgactcac gaatcacttg tagtcatgcc
     1441 cgctaatgtt tttatgcgat accgtgacag cttcatttca aaactgatcg aagttgctga
     1501 tgtaaagatc gcaaaagcat cgtcagaaat aatgaacgga actgtcgttc ttcacaaaaa
     1561 ccctctctgg atgcgtttct cgacatggtt tttcagccgg cttgaaacat tcggcgcatc
     1621 gtttttcggc aaagacctga aagtgaataa cgactgcaca aaatgcggac tctgcatgaa
     1681 aatatgcccg aaaatgaaca tcaccgcaga caaagaaggc agaccccggt tcggctggaa
     1741 gtgcatgatc tgcatgagat gcatctacaa atgcccgaat aacgcaatat caccccgtat
     1801 catgaaattt ttcaaagtaa aaaacttcta taaactcagc gagttacaaa aaccatccga
     1861 tttaaccaac cccaccgcat tcgaaaaagg gtttggaaag tatttcggag a
//
LOCUS       DAOOYB010000406         1874 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_79757_length_1874_cov_2.722375, whole genome shotgun sequence.
ACCESSION   DAOOYB010000406 DAOOYB010000000
VERSION     DAOOYB010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79757_length_1874_cov_2.722375"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="PL056_14000"
     CDS             <1..451
                     /locus_tag="PL056_14000"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HPM48174.1"
                     /translation="LTVKNDPRILPFGRFLRKAKINELPQIFNVFLGDMSIIGPRPQT
                     VKYFDYYPEDDQIEICKVRPGLSGLGSVVFRDEEEMCVETNGRTVEQIHVEDIIPRKI
                     KLERWYVANNSIWLDIKIIFATIWIVFFTESRLVEEMFKNIEEKSNE"
     gene            444..1556
                     /locus_tag="PL056_14005"
     CDS             444..1556
                     /locus_tag="PL056_14005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002191238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPM48175.1"
                     /translation="MNKRIPLSSPHMSEEGFEQEFVKEAFDTGWITPLGKNVDEFEKE
                     FAIKVGSSYAAALITGTAGIHLALKAAGVKNGDIVFCPTLTFSATANPIIYENAIPVF
                     IDSDYTSWNMCPVALEKAFEKYPNVKAVLVVHLYGLSADMDKIVEICKRHKVVLIEDA
                     AESLGAYYKGKHTGTFGDYGIFSFNGNKIITTSGGGMLVSENSEKIAKVRFWSTQSRD
                     KARHYQHSEIGFNYRMSNVLAGIGRGQLKVLEKRVAKKKHIFEFYKKELSNLQGIEFM
                     PVNDWNEPNFWLSCIQLNGKIRPIDIMEVLEKDNIESRPVWKPMHLQPFFEKYDHIGG
                     TVAQNLFENGVCLPSDTHMTDEDLTRVCKIIKGLWE"
     gene            1561..>1874
                     /locus_tag="PL056_14010"
     CDS             1561..>1874
                     /locus_tag="PL056_14010"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006862462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPM48176.1"
                     /translation="MGLINCPADFKFSVSMCVYINDNAAHFRESIESILNQTIKPDEV
                     ILVVDGPITPDLENVIKEYENGSFFKTIRLPKNVGHGEARRKGLENCSNDLVALMDAD
                     DI"
BASE COUNT          626 a          323 c          407 g          518 t
ORIGIN      
        1 gctcacagta aaaaatgacc cgcggattct cccgtttgga agattcttaa gaaaagcaaa
       61 aataaatgaa cttcctcaga tcttcaatgt gtttctcggc gacatgagca tcatcggtcc
      121 aaggccccag actgttaaat attttgacta ttatccggaa gatgatcaga tcgagatatg
      181 caaagtcagg ccgggacttt caggactcgg ctcagtagtt ttcagagatg aagaggagat
      241 gtgtgttgaa acaaacggca gaactgttga gcagatacat gtggaagata ttattccaag
      301 aaaaataaaa cttgaaagat ggtatgttgc aaataattcc atctggcttg acataaaaat
      361 aatttttgcc actatctgga tcgttttttt cactgaaagc agactggtgg aggaaatgtt
      421 taaaaatatt gaggaaaaat caaatgaata aaaggatccc gctctcttct ccccacatga
      481 gtgaagaagg gtttgaacag gaatttgtaa aagaggcgtt tgacactggc tggatcaccc
      541 ctcttggcaa aaatgttgat gaatttgaaa aagagttcgc aatcaaggtc ggctcttcat
      601 atgcggccgc attgataacc ggaactgccg gaattcattt agcattaaaa gcggcaggcg
      661 ttaaaaacgg agacatcgtt ttctgtccga cacttacttt ttcagcaact gcaaacccga
      721 tcatttatga aaatgcgata ccggttttta ttgatagcga ctacacttcc tggaatatgt
      781 gccctgttgc tcttgaaaaa gcatttgaaa aatatccaaa tgtgaaggcg gttcttgtgg
      841 ttcatcttta cggactcagt gccgacatgg ataagattgt tgaaatatgc aaaagacaca
      901 aagttgttct cattgaagat gcagcagaat ctcttggagc ctattacaaa ggaaagcaca
      961 ccggaacatt tggtgattac ggcatttttt catttaacgg aaacaagatc atcaccactt
     1021 caggaggagg aatgctggtc tccgaaaatt ctgaaaaaat tgcaaaagtg agattctggt
     1081 caactcagag ccgtgacaag gcgagacact atcagcactc tgaaatcggt tttaattatc
     1141 gtatgagcaa tgttcttgcc ggaattggga gaggacagct taaagtgctt gaaaaaaggg
     1201 tggcaaagaa aaaacatatc tttgaattct ataaaaaaga gctttcaaac cttcagggga
     1261 ttgaatttat gccggtcaat gattggaatg aacccaattt ctggctgagc tgtattcagt
     1321 tgaatggaaa gatcagaccg attgatataa tggaagtact tgaaaaagat aatattgaat
     1381 caagacctgt ctggaaaccg atgcatcttc aaccgttttt tgaaaaatat gatcatatcg
     1441 gcggaacagt tgcacaaaat ctgtttgaaa acggcgtctg cctcccaagc gacacccaca
     1501 tgaccgatga agatctgaca agagtctgca aaataataaa aggattgtgg gaataaagac
     1561 atgggtttga taaattgtcc cgcagatttt aaattttcag tttcaatgtg cgtatacata
     1621 aatgacaatg ctgcacattt tagagaatct attgaaagta ttttaaatca aacaattaaa
     1681 ccagatgaag ttattcttgt tgttgatggt ccaattactc ctgatttaga aaatgttata
     1741 aaagaatatg aaaacggttc gttttttaag acaatcagat tgcctaaaaa tgtggggcac
     1801 ggtgaagcca gaagaaaagg tttggaaaat tgttccaacg atcttgttgc ccttatggat
     1861 gctgatgata taag
//
LOCUS       DAOOYB010000407         1848 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_81408_length_1848_cov_5.614055, whole genome shotgun sequence.
ACCESSION   DAOOYB010000407 DAOOYB010000000
VERSION     DAOOYB010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81408_length_1848_cov_5.614055"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="PL056_14015"
     CDS             complement(<1..266)
                     /locus_tag="PL056_14015"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HPM48177.1"
                     /translation="MSYTTPFQWLKRVIRKYNRDIKNNQDIPGKEKEQLYLPEIPFHG
                     LRHTSATLLISEKVDVKTISSRLGHTLSILKNISQKTPCSSQIK"
     gene            425..619
                     /locus_tag="PL056_14020"
     CDS             425..619
                     /locus_tag="PL056_14020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48178.1"
                     /translation="MILTKNYLKKFQESMRISLPDNVEKKLLKEYGQPVVDDEGYISE
                     YTEQDIYEQIRKAVQGSKVG"
     gene            complement(1021..1119)
                     /locus_tag="PL056_14025"
     CDS             complement(1021..1119)
                     /locus_tag="PL056_14025"
                     /inference="COORDINATES: protein motif:HMM:NF024751.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48179.1"
                     /translation="MPPNSPDLNLIKSLWELLKTNVINNVFFPNFG"
     gene            1301..>1848
                     /locus_tag="PL056_14030"
     CDS             1301..>1848
                     /locus_tag="PL056_14030"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPM48180.1"
                     /translation="MKKRRTTLFLVLSFVLCLMLSACGSATSDTQASDAQGKSLGDRV
                     LRDRALADAPTYDPFKTNDVETMLVHYQIFESCFFEAQDGSYQPAICESYEFNEAGDV
                     LTMKIRDGVKFHNGSVVTPEDVAFSLNTAINSAYTAKVTSAMDYAEVVDGNVVLHLKF
                     PYASAIGCLVSSNCAIVPKDVY"
BASE COUNT          554 a          316 c          373 g          605 t
ORIGIN      
        1 tattttatct gagaagaaca gggcgttttc tgcgatatgt ttttgagaat tgacagagta
       61 tgccccaacc tggaagaaat tgttttgaca tcaacttttt cagatataag taatgtcgcc
      121 gatgtatgcc gcagcccatg aaacggtatt tcaggaagat aaagctgttc tttttctttt
      181 ccggggatat cctggttgtt tttgatgtct ctattgtatt ttcttataac tcttttcaac
      241 cactgaaacg gcgttgtata gctcattatt acaccgttcc attgagtaaa tagcagctta
      301 ttttcttccc atgccttatc ccatagatcg ccgcacttca gtttttgaat gttttgctca
      361 atcttatact taccttacag tcagtagcta cattaacata tgaagatgat ggagggaaac
      421 agatatgata ctgacaaaga attatctaaa aaagtttcaa gaaagcatga gaatatcgct
      481 tcctgataat gttgagaaaa agctattgaa ggaatacggt cagcctgtag ttgatgatga
      541 gggatatatt tccgagtaca cagaacaaga catatatgaa cagataagaa aggcggttca
      601 gggtagcaaa gtaggataaa attaattttc cgctattaat cgaagagatg aggaagtatc
      661 ttctacatca tacttccctc ccatctaata tattaaattt ctaataaaat agttaggagt
      721 taactgaagt taacataaaa gttccccacc ggtgtctagg gaaaaacatt tgcaaaagtg
      781 aggaattttc acttgcaaaa aacaaatatt tgtttaaaaa ccaagaatta aatgtttttt
      841 tgtttttcag acaaacacaa cgataattaa gttttgtata atttgtatag gaaaagtgtt
      901 tctttgttat agcaacggat aattcaataa gtattttgca gcctgccaca caaccttgtt
      961 atggtaatat cctgaatctt gttaattgaa tcactaaatc cgcgaacagc ggtccttatt
     1021 ttatccgaaa ttgggaaaga agacattatt tatgacattg gtcttcagca actcccaaag
     1081 tgacttgatt agattaagat caggactatt aggcggcaga aattccagct gaattcaact
     1141 caaaagttga ttttaaccta tttcttatga gtatgtcact tcaattttcc cttttctgta
     1201 gtaatacttg ggctgtagtt tatttattga attctaaata gttagcaaag ttgcgttagc
     1261 tacaccaaaa aaataaatta gattgttagg agggacccag atgaaaaaga gaagaaccac
     1321 attgtttctt gtactttctt ttgttctctg cctgatgctt tctgcctgtg gtagtgcgac
     1381 aagtgatacg caggcaagtg atgcgcaggg aaaatcatta ggtgatcgtg tgttgcgtga
     1441 ccgtgcatta gccgacgctc cgacatacga tccattcaag actaatgatg ttgagaccat
     1501 gcttgtacat tatcagattt ttgaatcgtg cttttttgaa gctcaggatg gcagctacca
     1561 gcctgctatt tgtgaatctt atgaattcaa tgaggcaggc gatgtgttga ccatgaagat
     1621 tcgtgatggt gttaagttcc ataatggtag tgttgttacg cccgaggatg ttgctttctc
     1681 cctgaatact gctatcaatt ccgcttatac agcgaaagtt actagcgcta tggattatgc
     1741 agaagttgtt gacgggaatg tagtgttgca tctgaagttc ccgtatgctt ctgctattgg
     1801 atgccttgtc agttcgaact gtgcgatcgt gcctaaggat gtttatga
//
LOCUS       DAOOYB010000408         1836 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_82197_length_1836_cov_8.935430, whole genome shotgun sequence.
ACCESSION   DAOOYB010000408 DAOOYB010000000
VERSION     DAOOYB010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82197_length_1836_cov_8.935430"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..1742)
                     /locus_tag="PL056_14035"
     CDS             complement(132..1742)
                     /locus_tag="PL056_14035"
                     /inference="COORDINATES: protein motif:HMM:TIGR01451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48181.1"
                     /translation="MLRNTITYLLIVLGFFSTLKAKELELDSSHSFWNVSYRSSMMFG
                     NLLDEEGKCLDVETGSTYTLNATFLPPDAYVMKAFLVWTGAQPIDKIDELADNEVILN
                     FKSEDGKIEKPKTVTATGYKVSQPQGFEFDSFVDGDSGKAYFTYRVDVTDFFKTLHEK
                     GQEFGNYNDGESLFGDYTVSGLDCSDDEIYLENNRNVSNWAIALVYSSYSFSRRSILF
                     LDGLHGYEPGSEIDPDGPVSCPTDASQDPNCTMLSNHGVDPFYFNHQILNWTLTNLLI
                     SHMDAPSSNFDIPDQPELVACTPANIPLNPDNPDSFWCPSGEHTFAIRIQNWGNSDAE
                     KIVVKAEIPKGMEYIRGTTEYADEFCIYQNKKHAEIFRNISDNENGFPLADGFNITEL
                     TGKLNPCPEDSNYLSCENFIIIRYKAKIKDSTPKHSVIESSANIETYNSNQGIPLKLR
                     LSTTDCVSSLDEINLDECGGIPFPCSEEEGCPDSEVQIDNDNEYNSDLDNETVSDFDD
                     KASVNDSDTETDGKDNSSGCGCSILGDQ"
BASE COUNT          551 a          363 c          327 g          595 t
ORIGIN      
        1 acccttttta tagtcctccc atttaagtat ctgcccttta taaactgaaa catcatggcc
       61 gagttccttt gatgaaacag ttgagaaaaa accaagaaca atgaaaagac aagtaattgt
      121 tttatttagc attattgatc tcctagaatc gaacaaccgc aaccggaact gttatctttt
      181 ccatcagttt ccgtgtcgga atcatttact gaagctttgt catcaaaatc cgaaaccgtt
      241 tcattatcca gatcggaatt gtattcatta tcattatcta tttgaacttc tgagtcagga
      301 caaccttctt cttccgaaca ggggaaaggt atgcctccac actcatcaag attgatttca
      361 tccagacttg aaacacagtc cgtagtcgaa agcctgagtt tgagaggaat tccctgattt
      421 gaattgtatg tctctatatt tgcggaactt tctatgacag aatgttttgg agtgctgtct
      481 ttaatctttg ctttgtatcg gatgataata aaattttcac aacttaaata attagagtct
      541 tcgggacacg ggtttaattt accggttaat tcagtaatat taaaaccatc tgcaagtgga
      601 aacccatttt cattgtcgga gatgttcctg aatatttcag catgtttttt attctgataa
      661 atacaaaatt catctgcata ctcagtggtt cctcttatat attccattcc tttgggtatt
      721 tcagctttaa caacgatttt ttcggcatca ctgtttcccc aattctgtat ccttatcgca
      781 aaagtatgtt ctcccgatgg acaccagaaa ctgtcaggat tgtcaggatt aaggggaatg
      841 tttgccggtg tgcatgcaac cagttccggc tgatccggaa tatcaaaatt tgaagagggt
      901 gcgtccatat gacttataag aagatttgtt aaagtccagt taagtatctg atggttaaaa
      961 taaaaaggat caacgccgtg atttgaaagc attgtacaat ttggatcctg agaagcatct
     1021 gtcggacagg agactggacc gtctggatcg atttctgatc ccggttcata accatgcaaa
     1081 ccgtctaaaa acaatattga tcttcttgaa aatgagtagg aagaatagac aagagctatt
     1141 gcccagttcg aaacatttct attgttttcc agataaatct catcatctga gcagtctaga
     1201 ccggaaacag tataatctcc aaaaagtgat tcaccgtcat tataatttcc aaattcctgt
     1261 cctttttcgt gaagtgtttt gaaaaaatca gtaacatcaa ctctataggt gaaatatgcc
     1321 tttcctgaat caccgtcaac aaatgaatca aactcaaatc cctgaggttg cgagactttg
     1381 tatcctgttg cagtaacagt tttgggtttt tcaatctttc cgtcttctga cttaaaattc
     1441 aatattactt cgttgtcagc taattcatca atcttatcaa tcggttgagc gcctgtccag
     1501 acaaggaatg ctttcatgac atatgcatca ggcggaagaa atgtggcgtt cagtgtataa
     1561 gtgcttcctg tctcaacatc aagacatttg ccctcttcat caagcagatt accaaacatc
     1621 atactgcttc ggtaagaaac attccagaaa gaatgcgatg aatccagttc aagttccttg
     1681 gctttcagag ttgagaaaaa accaagaaca attaaaagat aagtgatcgt atttcttagc
     1741 attatttgct ccataaattt gaacagccga agttgaaact attatctttt tcactgattt
     1801 ctttgtcatc atctgttaca gaatctgagt ctgtgt
//
LOCUS       DAOOYB010000409         1829 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_82682_length_1829_cov_4.316234, whole genome shotgun sequence.
ACCESSION   DAOOYB010000409 DAOOYB010000000
VERSION     DAOOYB010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82682_length_1829_cov_4.316234"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1829
                     /locus_tag="PL056_14040"
     CDS             <1..>1829
                     /locus_tag="PL056_14040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48182.1"
                     /translation="LFVPFFLFGLTTGWILRERKKEIGIYYSVNMTGSAFGAILFILL
                     FNSFNGQVVIPLITFLMSISALVCAVGLKEKIISAIVPVIIFPTLFFPALFPMDPPGD
                     KILGLVKDRANDVIYTGWSALSKVDVIKDPDRSAYPSIGLWGISPVFKEDGRIFPERM
                     GVVIDSWAYTSIFKYPQDFDFFDYFPTTLVYHLGGNNVSSLHIGAGGGMDLLAARHYG
                     VKNVTGIEINPVIVKAVQNEFKEYSGGIYSGNIEGIEIVVDEGRNFIESSNISYDIIQ
                     LSGVDTFSSTHAGAFALSENNLYSVEAFRSYYEKLNEDGYFTMTSWFAPDETGTPRFS
                     IRLLNIVRDSLTGMGIDPKNRVMFIVSDVYTLLVLKKGVFSYAEMLKGSEYIEKNKFV
                     PLVMPYKETVESNVFQKFLYADDAASEKLIDEYPYIVSPPTDDRPFFFELRKLNTLFA
                     GINSGVLPLNIFSGQTILFAILLILLFLGAMLILQPLYFFRLKNKTDLALYKLGYFSL
                     IGLGFMFVEIVLTQKLVLYLGHPSYSLSIALFSILLFSGLGSFFSNSRILRKRTTLFM
                     LCAILLAVIFLLSPTLHATLGLPFGIRVLITLFIIAPVSFLMGT"
BASE COUNT          480 a          373 c          388 g          588 t
ORIGIN      
        1 ctttttgtcc cttttttcct tttcggactc actacgggct ggatacttcg tgagagaaaa
       61 aaagagatcg gaatatatta cagtgtgaac atgaccggca gtgctttcgg agcgattctt
      121 ttcattttac tttttaacag ttttaacgga caggtcgtca tccctctgat aacttttcta
      181 atgtcaatat cggcacttgt ctgcgctgtc ggattaaaag agaagataat ttccgcaata
      241 gttccggtta tcattttccc tacacttttt ttcccggcac tttttccgat ggatccgccc
      301 ggcgacaaga tcctcggtct tgtcaaggac agagcgaatg atgtgatata taccggctgg
      361 agcgctcttt caaaggttga tgtgattaaa gatcctgaca gatccgccta tccatcgatc
      421 ggactttggg ggataagccc ggtttttaag gaagacggga gaatatttcc ggaaagaatg
      481 ggggtcgtta tcgattcatg ggcatataca tccatcttta aatatccgca agattttgat
      541 ttctttgact attttcccac aacgcttgtg tatcatctag gagggaacaa tgtctcatct
      601 ctccacatcg gtgcaggtgg cggaatggat ttgcttgccg ccagacacta cggtgtgaaa
      661 aatgtaacag gcattgaaat aaacccggtt atagtaaagg cggtgcaaaa tgagttcaaa
      721 gaatattcgg gcggcattta ttcaggaaac atagaaggaa tagagatcgt tgtcgatgaa
      781 ggaaggaatt tcatagaaag ttcaaatata agttatgata taattcagtt gtcgggagta
      841 gacactttct cttcaacaca tgcaggcgca ttcgccttgt cggaaaacaa tttatattca
      901 gtggaagcat tcaggtcata ttacgaaaag ctcaatgaag acggatattt cacaatgacc
      961 agctggtttg ctccggatga aaccggaact ccgagatttt cgatccggct tctgaacatt
     1021 gtaagagatt cgttgaccgg aatgggcata gaccccaaaa acagggtaat gttcatagtg
     1081 tcggatgttt acactcttct ggtgctcaaa aaaggggttt tctcatatgc tgaaatgctt
     1141 aaaggttctg aatacattga gaaaaacaaa tttgtccctt tagttatgcc atataaggag
     1201 acagttgaat caaatgtttt ccagaagttt ctttatgcgg atgatgcggc atcggaaaag
     1261 ctgatcgatg aatatcctta cattgtttct ccgcccacag acgacaggcc tttctttttt
     1321 gagctcagaa aactcaacac tctttttgca gggataaatt caggcgttct tccattgaac
     1381 atcttttctg gccagactat actttttgcg atacttctta ttctactgtt cctcggcgca
     1441 atgcttatac ttcaaccgct ttatttcttc agattgaaaa ataagacaga cctggctttg
     1501 tataaactcg gctatttttc gctgataggc ctcggtttta tgttcgttga aatagttctt
     1561 actcagaaac tggttctgta tctgggacat ccttcataca gtctttcgat agctcttttt
     1621 tcgatccttc ttttttcggg gcttggaagt tttttcagca acagcaggat cttaagaaaa
     1681 cggaccactc tttttatgct ttgtgcgata cttcttgccg taatatttct gctttccccg
     1741 acgcttcatg caactctcgg acttcctttc ggtatacggg ttctgataac gctatttata
     1801 attgctcctg tttcttttct tatggggac
//
LOCUS       DAOOYB010000410         1809 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_84247_length_1809_cov_2.144242, whole genome shotgun sequence.
ACCESSION   DAOOYB010000410 DAOOYB010000000
VERSION     DAOOYB010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84247_length_1809_cov_2.144242"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="PL056_14045"
     CDS             <1..613
                     /locus_tag="PL056_14045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48183.1"
                     /translation="DFIVKSFDVYEQILPGAKIIKYSAEIYPLRVFKQIVTIFCSYCP
                     SGFFRKEYPSITRYLLSKEERCFPDDIRIFAFFPLFEDGLIIRTSGLISKMYFDNDGN
                     SHNYNLSEFIFPPLGFFLTTGKAKIPNMYDLTFFNNFNYEDKIEIHFDIPFYPIVGWF
                     PGDYRNKAEIYEANAQFNTGPVAGSPTSISMREFVNYKNRRKS"
     gene            610..885
                     /locus_tag="PL056_14050"
     CDS             610..885
                     /locus_tag="PL056_14050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48184.1"
                     /translation="MTVERLMTAIGMRIFIKYYDYFKRGIDIINKLEEEGFTLKSCRS
                     RQSKAQRIFRENLQVEALKMVLNANKVSEETKNKAIELLRSEKNERN"
     gene            872..1528
                     /locus_tag="PL056_14055"
     CDS             872..1528
                     /locus_tag="PL056_14055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48185.1"
                     /translation="MNEIEKAIHCLTVYNEKFPSTKEIAEIEKNLCNRNVLKDFCAQE
                     DALKIAVLIKKFSSNIHVVIHSLGILTLLPEILEKDETIVSLSLGAGNTGRDFDLETT
                     GRVAEFKFVQWRGGAESIRQNNIFADFFKLVSCDTQKKKELYVVGDIPVDFLKKSKRK
                     ITSVFSKDQKIGEEFKKMYKDKNYEKVCDYYKDFKDVVKIKDINDLTDVFKMCEDKNI
                     "
BASE COUNT          636 a          278 c          377 g          518 t
ORIGIN      
        1 ggactttatc gtaaaaagtt ttgatgttta cgaacagatt cttcccggag caaaaattat
       61 taaatattca gcggaaattt atccgctcag agtattcaaa cagatcgtga caattttttg
      121 cagctactgt ccttctggtt ttttccgaaa ggaatatcca tctataacca gataccttct
      181 ttcaaaagag gagagatgtt ttcctgatga tataaggata tttgcttttt ttcctctttt
      241 tgaagacggc ttaataataa gaacttccgg gctcatttca aagatgtact ttgataatga
      301 tggaaactca cacaactata atttatcaga gtttatattt ccccctttag gatttttcct
      361 aaccacaggt aaagctaaaa ttccaaatat gtacgatctc acttttttca acaatttcaa
      421 ctatgaagat aaaatagaga tacattttga cattccgttt tatcctatcg tggggtggtt
      481 cccgggtgat tacagaaata aagcagaaat atatgaagct aacgcacagt ttaacaccgg
      541 acctgtcgca ggttccccga ccagtatttc aatgagagag tttgtaaatt ataaaaacag
      601 gagaaaatca tgaccgttga gagacttatg acagcaatag ggatgagaat ctttatcaag
      661 tattatgact atttcaagag aggaattgat atcatcaaca aactcgaaga ggaagggttc
      721 acacttaaat cctgcagatc gagacaatcg aaagctcaaa gaattttcag agaaaatctc
      781 caggttgaag ctctgaaaat ggtattaaat gccaacaagg tgtcagagga gacaaaaaat
      841 aaggcgatag aattgcttag gagtgaaaaa aatgaacgaa attgaaaaag caatacattg
      901 tttaacagtg tataatgaaa aattccctag cacgaaggaa attgcagaaa ttgagaaaaa
      961 cctttgtaac aggaatgtct taaaagattt ttgtgctcaa gaagatgcat tgaaaatagc
     1021 cgttttgata aagaaatttt ctagtaatat acatgttgtt attcactcgc ttggtatttt
     1081 aacacttctt cctgaaatat tggaaaaaga cgaaacaata gtatcacttt ctttgggagc
     1141 aggcaataca ggaagggatt ttgatcttga aacaacggga agagttgccg aatttaagtt
     1201 tgtgcaatgg cggggtggag ctgaaagcat acgccagaac aacatttttg cagatttctt
     1261 taaacttgta tcttgcgata ctcaaaaaaa gaaagaactg tatgttgttg gtgatattcc
     1321 tgttgacttt cttaagaaaa gcaaacggaa gattacaagt gttttcagta aggatcaaaa
     1381 aatcggggaa gagtttaaga agatgtataa agataaaaat tatgaaaaag tttgtgatta
     1441 ttacaaggat tttaaggatg tagttaaaat aaaagacatc aatgacctca cggatgtttt
     1501 taaaatgtgc gaagacaaaa acatctagat aattcaaata ctaacacccc aattcccaca
     1561 taccccacat tttaattttc cctgtgataa tctgttgctg ttttcattgc ggaaggtgaa
     1621 ccgaagaact gtaaaaaagg gggttgaaat gagcggagag atcaggcgga tcatcagttt
     1681 gagggatggg aggatggcgg ttattcctaa gggcggggat gatctaaaga ttaggatcct
     1741 agacaataac gggaagctga tgttcgagct gggggagaac aagtgctatg acggggcgaa
     1801 actgagtgc
//
LOCUS       DAOOYB010000411         1764 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_87503_length_1764_cov_8.792276, whole genome shotgun sequence.
ACCESSION   DAOOYB010000411 DAOOYB010000000
VERSION     DAOOYB010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87503_length_1764_cov_8.792276"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1231
                     /locus_tag="PL056_14060"
     CDS             <1..1231
                     /locus_tag="PL056_14060"
                     /inference="COORDINATES: protein motif:HMM:NF015642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HPM48186.1"
                     /translation="KETVMNHDTERNTCQFNQQKGGAMNRDTYKQDKNKAQKRNELIL
                     SEFFGWLLLKGLSVKTMKKHEDNMRFYINDYLLFYCALPPEEGVDRIDEFMRDWLPEN
                     ALWVSKTAIKEYCVVLKKFYTFIYEIGYVSKSDLDTVKHIIKDQMDDWVDGVETHYDD
                     MNREIVDDYLSAMEYSLEEYLDDIDMGTLMEFVKRHKHFKSFDAFHGTACGFAALPLS
                     SDYDSNRELFIGNMMQNVLCSLEEKKRFEEALMTFYDRTEDIIFAGDFVPYCGSWEFK
                     DIKTNDPKDWCIGFMAAFSLFKDLVRISDRDYLEIFQDGLSPLMLLSFDQNNPDFLMV
                     KNKIIEKEGSWESFQERMFRHLPDIPLLFADIAEEFMEEFEEKKNAAPVRTEKVGRND
                     SCPCGSGKKYKKCCGSK"
     gene            complement(1298..1519)
                     /locus_tag="PL056_14065"
     CDS             complement(1298..1519)
                     /locus_tag="PL056_14065"
                     /inference="COORDINATES: protein motif:HMM:NF018958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HPM48187.1"
                     /translation="MASNNDVLKKLRIALQLEEDDLLEIFALAEFPVTKPQLSAMFRK
                     KGHKHYKECQDQMMKKFLTGLALKFRSGK"
     gene            complement(1509..1736)
                     /locus_tag="PL056_14070"
     CDS             complement(1509..1736)
                     /locus_tag="PL056_14070"
                     /inference="COORDINATES: protein motif:HMM:NF018958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HPM48188.1"
                     /translation="MDNNDILKKVRYALDLKDSAMVKMFKSGGVEMSRLELAAAFGDE
                     EDPGYRKLSNEELLSFLDGLILDRRGDKNGK"
BASE COUNT          549 a          299 c          378 g          538 t
ORIGIN      
        1 aaaggaaact gtaatgaacc atgatactga aagaaatact tgccagttta atcagcaaaa
       61 aggaggggca atgaataggg acacatacaa acaagacaaa aataaagcac aaaagcgtaa
      121 tgagttaatt ctttctgagt ttttcgggtg gcttttatta aaagggcttt ctgtaaaaac
      181 gatgaagaag catgaggaca acatgcggtt ttatatcaat gactatctgc ttttttactg
      241 tgcgttgcca cctgaagaag gtgttgaccg gatcgatgaa ttcatgagag actggttacc
      301 tgaaaatgca ctgtgggtaa gcaaaacagc tataaaggaa tattgtgtcg tattgaagaa
      361 attttataca ttcatatatg aaataggata tgtgagcaaa agcgatcttg atacggttaa
      421 gcatattata aaagatcaga tggatgactg ggttgacggt gttgaaacac attatgatga
      481 tatgaatcgt gaaatcgtcg atgattattt aagtgcaatg gaatactccc ttgaagaata
      541 tcttgatgat attgatatgg gcactctcat ggagtttgta aagcggcata aacattttaa
      601 atcctttgat gcttttcacg gtacagcatg cgggtttgca gcacttcccc tttcttcgga
      661 ttacgattct aacagagaac tttttatcgg aaacatgatg cagaatgttt tatgttcact
      721 tgaagagaaa aagagatttg aagaagctct tatgactttt tatgatcgca cggaagacat
      781 tatatttgct ggagattttg tcccttattg cggatcatgg gagtttaaag acattaaaac
      841 gaatgacccg aaagattggt gcatcgggtt tatggcggca ttttcacttt tcaaggattt
      901 agtgaggatt tcagaccgtg attatcttga gatttttcag gatggacttt ctccgctgat
      961 gctgctgagc tttgatcaaa ataaccctga ttttttaatg gtaaaaaaca agatcattga
     1021 gaaagaaggg tcgtgggaat cttttcagga aaggatgttc agacatcttc ccgacatccc
     1081 attgttgttt gcagacatag cagaagagtt tatggaagaa tttgaagaga aaaaaaatgc
     1141 ggctcctgtc agaactgaaa aagtaggaag aaatgattct tgtccctgcg gaagtggaaa
     1201 aaagtataaa aaatgttgtg gatcaaaatg attctattaa aaacatttgg aaaccaaaac
     1261 tgttgaaggc ggggagggaa agcaatgaaa ggaaatgtta cttccccgat ctgaatttta
     1321 atgcaagtcc ggtaagaaat tttttcatca tctgatcctg gcactcttta tagtgcttgt
     1381 gtcctttttt cctaaacatt gctgacaact gcggttttgt cacaggaaat tcagccagag
     1441 cgaatatttc aagaagatcg tcctcttcaa gctggagagc aattctcagt tttttcaaca
     1501 catcgttgtt acttgccatt tttatcacct cttctgtcaa ggatgagtcc gtcaagaaaa
     1561 ctcagaagtt cttcatttga cagttttcta tatcccggat cttcctcatc gccgaatgcc
     1621 gcggcaagtt cgagacggga catttcaacg cccccgctct tgaacatctt gaccattgct
     1681 gaatctttca ggtcaagtgc ataccgcact tttttcaaga tatcattgtt atccatagtt
     1741 gtcccctctt tctgaattat ttag
//
LOCUS       DAOOYB010000412         1730 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_90202_length_1730_cov_6.722985, whole genome shotgun sequence.
ACCESSION   DAOOYB010000412 DAOOYB010000000
VERSION     DAOOYB010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90202_length_1730_cov_6.722985"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            280..1410
                     /locus_tag="PL056_14075"
     CDS             280..1410
                     /locus_tag="PL056_14075"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HPM48189.1"
                     /translation="MRFLLIALLFLSVVSCNTSSSGNYKPVSDETVDETVDETVDDLS
                     DEALAESEDTESDENTDKNTDEGVDETVDETTDEATDENTDENTDEGVDETADETADE
                     TADETVDETVDETVDETVDESFDEDTIPDDDPDDDPYASTNGLYDESLREALYNLIAV
                     DSPFDYDTARDAMYGGIDVFNDEIECLYSGRTIPGPATRSEVYNGGSGLNCEHSWPKS
                     QIVSSNGENTPPGYDIHHLFPTDVQVNSDRGTLDFGEVSMMTDTYCVVGSDCSYRGQS
                     DSGSIVFEVRDSRKGDIARAHFYMAVRWAFECDIYLCIDDNGDMIDGRIKVAEEAVLR
                     QWHVDDPVDERERTRNDRIEAAQGNRNPFVDRPDLVERLSDF"
     gene            complement(1447..>1730)
                     /locus_tag="PL056_14080"
     CDS             complement(1447..>1730)
                     /locus_tag="PL056_14080"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HPM48190.1"
                     /translation="TPNQIEYAAEMGLEHHLGLTCDPVCGLVQIPCIERNAFAASRAL
                     DANTFALLSDGKHTISFDTIVETMNRTGNDLPMIYKETSEGGLAVTAKR"
BASE COUNT          471 a          320 c          446 g          493 t
ORIGIN      
        1 gatctataaa atggctcaat tagattagac caatcattat tttctgctat cccttcacac
       61 tctcaactat cgggatgatg ttttcttttg atttgaaatt gcaacaataa aaaaacatgt
      121 caaatttgct agagaaattg acgaaaatcc tgtgtttcta ttgtcatctg tcggttttta
      181 tatcagtgcc gagtttgtca agatggtgtc aaactgtatt ttcaggaatc tttcaagtat
      241 aatcattttg ctcgttcttg cataatttgg aggtatgaaa tgagatttct tttaattgca
      301 cttctttttt tgagtgttgt ttcctgtaat acttcttcaa gtggaaatta taaaccggtc
      361 tctgacgaaa ctgtggacga aactgtggac gaaacagttg acgacttgtc cgacgaagcc
      421 ttggcggagt cggaagatac ggagtcggat gaaaacaccg acaaaaacac cgatgaaggt
      481 gttgacgaaa ccgttgatga aactactgat gaggccaccg acgaaaacac cgatgaaaac
      541 accgatgaag gtgttgatga aactgcggat gaaactgcgg atgaaactgc ggatgagact
      601 gtggatgaga ctgtggatga aacagtggat gaaacagtgg atgaatcctt tgatgaagat
      661 acgattcctg acgatgatcc tgacgatgat ccttacgcat caacaaacgg gctttatgat
      721 gagtctttgc gtgaggcgct gtacaacctg attgcagttg acagtccgtt tgattatgat
      781 actgcccgag atgcaatgta tggtggaatt gatgtgttta atgacgaaat cgaatgtctg
      841 tacagcggca ggacaattcc aggtcctgca actagaagcg aagtgtacaa cggtggcagt
      901 ggactaaact gtgagcattc gtggcccaaa agtcagattg tttcaagtaa cggtgagaat
      961 acgccgccgg gatacgatat ccatcatctg tttcctacag atgtgcaggt gaattcagat
     1021 cggggaacac ttgatttcgg tgaagtttcc atgatgacgg acacttactg cgtcgtggga
     1081 agtgactgct catacagagg gcagagtgat tccggatcca ttgtgtttga ggtgcgtgat
     1141 tcccggaagg gcgatattgc gagggcacat ttttatatgg cggtccgctg ggcgttcgaa
     1201 tgtgatatct atctctgcat tgatgataac ggagatatga tagacgggcg gattaaagtc
     1261 gcggaagaag cggttcttcg gcagtggcat gttgatgatc cggtggatga gcgggaacgc
     1321 acgagaaatg atcgcattga agctgctcag ggaaatagaa atccctttgt tgatcgacct
     1381 gatctcgtgg agcgtctgtc ggatttctga tgaattcaga aaatcaaaaa aggtcataac
     1441 gggattttat cttttcgcag taacagcaag tccgccttct gaagtttcct tgtagatcat
     1501 gggaagatca tttcctgtcc ggttcatggt ttcaactatt gtgtcaaaac ttattgtgtg
     1561 ttttccgtct gaaagaagag caaatgtgtt tgcgtcaagt gctcttgatg ccgcgaaggc
     1621 atttctttca atgcacggga tctggacaag tccgcaaacg gggtcgcatg tcagcccgag
     1681 atggtgttca agtcccattt cagcggcata ttcaatctgg ttgggagttc
//
LOCUS       DAOOYB010000413         1717 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_91240_length_1717_cov_9.530084, whole genome shotgun sequence.
ACCESSION   DAOOYB010000413 DAOOYB010000000
VERSION     DAOOYB010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91240_length_1717_cov_9.530084"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..590
                     /locus_tag="PL056_14085"
     CDS             18..590
                     /locus_tag="PL056_14085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HPM48191.1"
                     /translation="MSKNIVIIDNYDSFTYNLYQFFANEGNCSVFKNDEITLAELKKK
                     EMTHLVISPGPGRPEDSKVSIEAVQFFKDKIPILGVCLGMQLMAYLSGGKIEKHPPVH
                     GSTEKIFHISSGIHSAMAQGFLAARYNSLCVTDIDETKLEITARGESGFIMGLRDIKY
                     PLFEGVQYHPESFLTEGGSRILKNFLELKN"
     gene            complement(622..1329)
                     /locus_tag="PL056_14090"
     CDS             complement(622..1329)
                     /locus_tag="PL056_14090"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009746669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HPM48192.1"
                     /translation="MRKFLILSAVFTLLLLSTITLVSNQKKDEKKSGEAPFVQPELPY
                     AYNALEPYVDAKTMEIHYTKHHAGYTANLNNAAKGTKYCKMPIEQVLKELDMNNAALR
                     NNAGGYYNHNLFWEIMGPGKGGKPTGELAKAIDKSFGSFDKFKAELSKAAATQFGSGW
                     AWLNVEKDKKLSICATPNQDNPLMPGTKCKGVPVLGIDVWEHAYYLKYQNKRGDYIEA
                     FFSVINWDKVSEKFLKN"
     gene            complement(1344..>1717)
                     /gene="tpx"
                     /locus_tag="PL056_14095"
     CDS             complement(1344..>1717)
                     /gene="tpx"
                     /locus_tag="PL056_14095"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016776442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HPM48193.1"
                     /translation="GKVRIISVFPSIDTPVCAAQTRRFNVEAANLGDVQILTVSCDLP
                     FALGRFCAAEGIDKEITLSDHKELDFGLKYGFAIDELRLLSRGVVVVDKDGVVKHVEY
                     VKEITEEPDYDSAIEAVKKII"
BASE COUNT          474 a          360 c          347 g          536 t
ORIGIN      
        1 actggccaaa tccgtaaatg tcaaaaaaca tcgtaataat agacaattac gatagtttca
       61 catataacct ttatcagttt ttcgctaatg aaggaaactg ttcagttttc aaaaatgatg
      121 agataactct cgctgaattg aagaagaaag agatgaccca ccttgttatt tcccccggtc
      181 cgggaagacc tgaagattca aaggtttcaa ttgaggcggt tcagtttttc aaagataaga
      241 tccctatact cggcgtgtgt cttggaatgc agctgatggc ttatttaagt ggcggaaaga
      301 ttgaaaaaca tccgcccgtt cacggttcaa ctgaaaaaat ttttcacata agttccggaa
      361 ttcattcagc aatggctcag ggatttcttg ctgcaagata taattcactc tgtgtcacag
      421 atattgatga aacaaaactt gaaataacag cccgcggcga aagcggattt atcatgggac
      481 ttcgtgacat aaaatatccg ctttttgaag gtgttcagta ccaccctgaa tcgtttctga
      541 ccgagggtgg gagcagaata ttaaagaact ttttagaact taagaactga catatattca
      601 atgagttgga aaaaacagct gtcagttttt caggaatttt tcagaaactt tatcccagtt
      661 gatgacactg aagaatgctt cgatatagtc gcctctttta ttctgatatt ttaagtaata
      721 tgcatgttcc cagacatcaa ttccaagcac cggaactcct ttacattttg ttccgggcat
      781 taaagggttg tcctgattcg gtgtggcaca gatcgaaagt tttttatcct tttcaacatt
      841 aagccatgcc catcccgatc cgaactgagt tgctgccgct tttgaaagct ctgctttgaa
      901 tttgtcaaat gatccgaatg acttatcaat cgcttttgca agttctccag tgggttttcc
      961 ccctttgcca ggtcccatta tctcccagaa aaggttgtgg ttgtaatatc cgccggcatt
     1021 gtttctcagc gccgcattat tcatatccaa ttccttgagt acctgttcga taggcatttt
     1081 gcaatatttt gtccctttag cggcgttatt gaggtttgca gtatatccgg cgtgatgttt
     1141 tgtgtaatgg atttccatgg ttttagcatc aacatacggc tcaagagcat tgtaggcgta
     1201 aggcagttcc ggttgtacaa aaggtgcttc accgcttttt ttttcatctt ttttctggtt
     1261 gcttaccaga gttatcgtgc tcagcagcag cagtgtaaaa actgctgata aaataaggaa
     1321 ctttctcata acctctcctc ctgttaaatg atctttttta ccgcttctat tgccgaatcg
     1381 taatcgggtt cttcggttat ttccttaaca tattcaacat gtttcacaac gccgtcttta
     1441 tcaacgacaa caacgccgcg tgaaaggaga cggagttcgt caattgcaaa accgtatttc
     1501 agtccgaaat caagttcttt atggtctgaa agtgtaattt ctttatcgat cccttcagcg
     1561 gcacagaatc ttccaagagc aaatggaaga tcacacgaaa ccgtgagtat ctgaacatcg
     1621 cctagatttg cggcttcgac attgaatctg cgtgtctgag cggcacagac cggtgtgtca
     1681 attgacggga aaactgaaat aatacggacc tttccat
//
LOCUS       DAOOYB010000414         1696 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_92986_length_1696_cov_5.217550, whole genome shotgun sequence.
ACCESSION   DAOOYB010000414 DAOOYB010000000
VERSION     DAOOYB010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92986_length_1696_cov_5.217550"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            478..1431
                     /locus_tag="PL056_14100"
     CDS             478..1431
                     /locus_tag="PL056_14100"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPM48194.1"
                     /translation="MDQKDVDQQSVEEQENPDSPVETSPAVTDDVVGGCFTIIFTGIV
                     LILAFVFRAEILNTACYFDLSVACVLSADLYTDNDEMKLEKYVNACNGKWVWKEKGCY
                     KAGLMYEKKSINSGTEDQSENLLKAHLYYKMGCPDYGDEDEGACLKLVELKLLFQKEE
                     QKADLYEKGYAKYEQKDYNGAIVDLSELIKISPNDPHAYNLRGISKSELGKYESAIED
                     YNKVIELLPDNHIGYYNRALSLIFLDRFEDAKKDYETAKKLKLDDFAVNNLPIMGLIL
                     EQKYDEAKAKVKELKDAGVEVDTDYEKILSSKFSAPSPIKL"
     gene            1482..>1696
                     /locus_tag="PL056_14105"
     CDS             1482..>1696
                     /locus_tag="PL056_14105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48195.1"
                     /translation="MKFFAVALFLLPVFLFSEKMMTEDEFKKTLYEGEKKEGEIVDVA
                     QEEDDTEDLSKVGLKDLEKGCSLKEES"
BASE COUNT          509 a          279 c          378 g          530 t
ORIGIN      
        1 ctttataggg ggtattgtaa ttctggagtc aaatatgctt gtgacaagta tgctgaatta
       61 tatgatatgg gctttggcat cgaagactga atatttttac tgccgtcttc cccttccctt
      121 ccagactccg cacacgctcc ggttattcct tgccggaatg aactgacctt gcggtcacct
      181 tacagtcaag ggacggttta acttcactgt tcaccgctca cgcttcactg ttcacggcag
      241 ccatcggctg cgttgatatt gcatatagct ctcacttttt tggtattttt attagcggtc
      301 tcacgacggc acatcctctt tgcttaaaat ggggcaacgc tccgcaggga gcctttcctt
      361 aatccatttt aagtcccact gaaggtctcc cccctttatt tccagaaaac tttaattctg
      421 atttgaatct gttaaaatgc ttccgttgtg tttaattttt tatcctggag gtctattatg
      481 gatcagaaag atgttgatca gcaatctgtt gaagagcagg aaaatccaga ttccccggtt
      541 gagacatctc cggcagtaac agatgatgtt gttggtggtt gtttcacgat tattttcact
      601 ggtatcgttt taatccttgc ttttgttttc agggcggaaa ttctaaatac agcatgttat
      661 tttgatcttt cagtagcatg tgttttgagt gcggatcttt atactgataa cgatgaaatg
      721 aaactggaaa agtatgtaaa tgcctgtaat gggaaatggg tatggaaaga gaaagggtgc
      781 tataaagctg gactcatgta tgagaagaaa tctatcaata gcgggactga agatcagagt
      841 gaaaatttat taaaagcaca cctgtattat aaaatgggat gtcctgatta tggggatgaa
      901 gatgaaggtg cctgtcttaa actggtagaa cttaagcttt tatttcagaa agaagaacaa
      961 aaagcagatt tgtatgagaa ggggtatgct aaatatgaac agaaagacta taatggtgca
     1021 attgtagatc taagtgagct aattaaaatt tcgccgaatg acccccatgc ttataatctc
     1081 aggggaattt ctaagtccga actgggaaaa tatgaaagtg cgattgaaga ctacaataaa
     1141 gtaattgagc ttcttcctga caaccatatc ggatattata atcgagcatt gtcgttgatt
     1201 tttttggata ggtttgaaga tgccaaaaaa gattatgaga ctgctaaaaa gcttaaatta
     1261 gatgactttg ctgtgaacaa ccttccaata atgggcctta tccttgagca gaaatacgat
     1321 gaggcaaagg ctaaggttaa agagttaaaa gatgctggtg tagaagttga tacagactat
     1381 gaaaaaattc tgagttctaa attttccgcc ccctccccca taaaacttta attctgattt
     1441 gaatctgtta aactctttaa gttactgaaa ggggggcatt tatgaaattt tttgcggttg
     1501 ctttgttttt gttaccagtt tttcttttct ccgaaaagat gatgacagag gatgagttca
     1561 aaaagacact gtatgaaggc gaaaagaagg aaggtgaaat agtggatgta gcacaggagg
     1621 aagatgatac ggaagatctt tcaaaagttg gtttgaaaga tttggaaaaa gggtgttcat
     1681 tgaaggaaga atcttt
//
LOCUS       DAOOYB010000415         1675 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_94783_length_1675_cov_5.316049, whole genome shotgun sequence.
ACCESSION   DAOOYB010000415 DAOOYB010000000
VERSION     DAOOYB010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94783_length_1675_cov_5.316049"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(141..>1675)
                     /locus_tag="PL056_14110"
     CDS             complement(141..>1675)
                     /locus_tag="PL056_14110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48196.1"
                     /translation="LPDTVEYVAESTEIATEFHDGIGTNWKKIDDVKKEFPFKNTVKV
                     AELMGYCDKATFECQDTIMVRFVVKPKIDLPKYKIILNSAEISDAGKIVPYNTNSFVP
                     LRLKNGNCPPITECNLPPKSECGGAPMPCCNECHLDTDCDEGMCCNEAGFCQKDPCVT
                     TRKCHDSEIMVLVGKNSTPNNVFFISPQINFVLGQLSLFELSGEECFFNIDSLKLKFD
                     LSDKNISLSNIKLVNDFNGNGKVDGEDLPLSSVDKLKDGYADFPFSDPNNRIWAYFKN
                     NLLFVADVDYAENKQITNNSTFTPSIEEGGLTIVDAGTPEISGLPIIFSKFQMVPENT
                     FAIMKGEEDSQYSSNLFFNYNDSELLQFKAFSNGRNDMIKSITITIPDGGTLNFGKGI
                     HSISIFYDKYNGGTNEELLIRTEKTDSKTSHKFNLDIPFEDGVWKYFVIKADVDVDEE
                     DYFQIQISDIEIESNNEILGLPLNSKEYYYKCDPMYSDCYDPGPVPPCGIFAVSCSFL
                     FL"
BASE COUNT          545 a          318 c          251 g          561 t
ORIGIN      
        1 acccttttta tagtcctccc atttaagtat ctgcccttta taaactgaaa catcaggtcc
       61 aaattccttt gatgaaacag ttgagaaaaa accaagaaca atgaaaagac aagcaattgt
      121 ttttataagc attatcgacc tcacaaaaat aaaaaagaac acgaaacagc aaaaatcccg
      181 catggtggaa caggaccggg atcataacaa tctgaataca tcggatcaca tttatagtaa
      241 tattcctttg aatttaaggg aagtcctagt atttcattat ttgattctat ttcgatatca
      301 gatatctgaa tctgaaaata atcttcttca tcaacatcaa catctgcttt tataacaaaa
      361 tatttccaaa caccatcttc aaacggaata tccagattga atttatgcga tgtcttgctg
      421 tctgttttct ccgtacggat taataattct tcattagttc cgccattata tttatcataa
      481 aaaatagaaa tagagtgtat ccctttacca aaattcaaag tcccaccatc aggaattgta
      541 attgtaatac tctttatcat gtcgtttctg ccatttgaaa atgctttgaa ctgtaatagt
      601 tcagaatcgt tataattaaa gaaaagattg cttgaatatt gagaatcttc ctctcctttc
      661 ataattgcaa aagtgttttc cggaaccatt tgaaatttgc tgaaaattat cggcaaccct
      721 gatatttcag gtgttcctgc atcaacaatt gttaatcctc cttcttcaat tgatggagtg
      781 aaagtagaat tatttgttat ctgtttgttt tcagcgtaat caacatcagc aacaaaaaga
      841 agattatttt tgaaataagc ccaaatgcgg ttgttcggat ctgaaaatgg aaaatcagca
      901 taaccgtctt taagtttatc aactgaactt aaaggtaaat cttcaccatc aacctttccg
      961 ttgccattga aatcattgac aagttttatg ttgcttaatg aaatattttt gtcacttaaa
     1021 tcaaatttga gtttcaggga atcaatatta aaaaagcact cctctccgga caactcaaat
     1081 aaagaaagtt gacctaagac aaaatttatc tgtggagaaa tgaaaaatac attatttggt
     1141 gtagagtttt tgccgacaag aaccataatt tcactatcat gacattttct tgttgtgacg
     1201 cagggatctt tttggcaaaa tcctgcttcg ttgcagcaca ttccttcatc acagtctgta
     1261 tccagatgac actcattaca acaaggcatg ggagctccgc cgcattcact ttttggagga
     1321 agattacatt ctgttattgg tggacaattt ccatttttta accttaacgg aacaaaacta
     1381 ttcgtgttat aaggaactat ttttccagca tcagatattt cagcactgtt caagatgatc
     1441 ttgtactttg gaagatcaat ttttggctta acgacaaatc tgaccatgat tgtatcctga
     1501 cattcaaaag tagctttatc acaatatccc attaactcgg ctactttcac agtgtttttg
     1561 aacggaaact ctttttttac atcatcaatt ttcttccaat ttgttccaat tccatcgtga
     1621 aattcggttg ctatctctgt tgattcagct acatactcaa ctgtatcagg cagag
//
LOCUS       DAOOYB010000416         1672 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_95085_length_1672_cov_2.714904, whole genome shotgun sequence.
ACCESSION   DAOOYB010000416 DAOOYB010000000
VERSION     DAOOYB010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95085_length_1672_cov_2.714904"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..650
                     /locus_tag="PL056_14115"
     CDS             <1..650
                     /locus_tag="PL056_14115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48197.1"
                     /translation="QECNTTDKTAEQYQSKFKEGVRLVFYDKVDPTPRKDEKEARSET
                     ETFKNAAETIVDDYKEIDKGTKVKTVSLQINSREEIQSAIEKQELGSIVSMDIISHSG
                     SDKKKLYFSAIFNTKNYLQPKQIVADRFNPKAVIELHGCNSGLEGVVGGIGKDISQKL
                     PEATVIGHATKSNPNHPHSNLSDYRHGKVNVYKGGELKETGQREKMKIHWNSAEK"
     gene            670..>1672
                     /locus_tag="PL056_14120"
     CDS             670..>1672
                     /locus_tag="PL056_14120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48198.1"
                     /translation="METVNKINLSKVLALLMFSLISLCCQRKNVSALSDVIIEDLSFS
                     FKDISGEIELNQDINGDVYAYSYSQDHKKIIFYKLVDRQWLFVTEYDYSEKESPYHQL
                     VINNGKMLLTFYKNDDSCAEEGYLINNILKYENNKWENVELSFRRDFSVPPVARFDRH
                     GNIFLVSFLDTQIYTYNEAKWMKKKSTPPCDELNISSEPLYFCDRNTCYCICTNDISF
                     KTDIIEKDFYIFKENDKEWEKISNLPRDNYFTISYFGYIKDSFFVTFYRKDFFRCENG
                     LEKSVKECYPHVIAFRNGKWLEIKLPEELKKEVHVFSDYKSRFLFAATHIDRNIHLYY
                     "
BASE COUNT          620 a          235 c          318 g          499 t
ORIGIN      
        1 aacaggagtg taatacaaca gataagactg cggaacagta tcagtcaaag ttcaaagaag
       61 gtgtgaggtt ggttttttat gacaaagtag acccgacacc tagaaaagat gagaaagagg
      121 caagaagtga aactgaaaca tttaaaaatg ccgccgaaac aattgttgat gactacaagg
      181 aaattgataa gggaacgaaa gttaagacag tttctctaca gattaatagc agagaggaaa
      241 tacaaagtgc aattgaaaaa caggaactag gatcaatcgt ttcaatggat attattagtc
      301 acagcggatc tgataaaaag aaactatact tttctgcgat ttttaatact aagaattatt
      361 tgcaacctaa acaaattgta gcagaccggt ttaatccaaa agctgtaatt gagcttcatg
      421 gttgtaattc tggacttgaa ggtgttgtgg gaggaatcgg aaaagatatt tcccaaaagc
      481 ttcccgaagc aacggttatt ggacatgcta caaaatctaa tccaaatcac cctcactcaa
      541 atttatctga ttatcgtcat ggaaaagtaa atgtatataa aggtggagaa ctcaaagaaa
      601 ctggacaaag ggaaaaaatg aaaatacatt ggaattcagc agaaaaatga tactatcagg
      661 gaggttttat tggaaacagt caacaagatt aatctaagca aagttctcgc attattgatg
      721 ttttcactaa tttctttgtg ttgtcaaaga aagaatgttt ctgctttgtc tgatgtaatt
      781 attgaagacc tctctttttc atttaaagat atttcaggag aaattgagtt aaatcaagat
      841 attaatggcg atgtttatgc ttactcgtat tcacaggatc acaaaaaaat catattttac
      901 aaactggttg acaggcagtg gctttttgta acagaatatg attattcaga aaaggagtct
      961 ccttatcatc aacttgtaat aaataacggg aaaatgctac taacatttta taaaaatgac
     1021 gattcttgtg ctgaagaggg gtatctaata aataacattc tgaaatacga aaataataaa
     1081 tgggagaatg ttgaactaag ttttcgaaga gatttttctg ttcctcctgt tgccagattc
     1141 gatagacatg gcaatatatt cctcgtatcg tttcttgata cacaaatata tacttataat
     1201 gaagcaaaat ggatgaagaa gaaaagcaca ccaccttgtg atgagcttaa tataagtagc
     1261 gaaccccttt atttctgtga tagaaataca tgctattgta tttgtacaaa tgatataagt
     1321 tttaaaactg atatcattga gaaggatttt tatattttca aagagaatga caaagaatgg
     1381 gagaaaatat ctaatttgcc acgggacaac tatttcacga taagctattt tggatatatt
     1441 aaggactcgt tttttgtaac cttttataga aaggattttt tcagatgcga aaatggacta
     1501 gaaaagtcag ttaaagaatg ctacccgcat gtaattgctt tcagaaacgg gaaatggcta
     1561 gagataaaac ttccagagga attaaagaaa gaagttcatg tttttagtga ttataagagc
     1621 cgatttttat ttgcagctac ccatattgac cgaaacatac atctttatta tt
//
LOCUS       DAOOYB010000417         1658 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_96270_length_1658_cov_7.623206, whole genome shotgun sequence.
ACCESSION   DAOOYB010000417 DAOOYB010000000
VERSION     DAOOYB010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96270_length_1658_cov_7.623206"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..899)
                     /locus_tag="PL056_14125"
     CDS             complement(120..899)
                     /locus_tag="PL056_14125"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HPM48199.1"
                     /translation="MTILESIILGLLQGFTEFLPVSSSGHLALTENLMGHKDVPLIYD
                     ILLHVASLMAVMIFFRRKIADLFNGMTNFKEYNAQHKYLVLLIISTVITGGMVFITKP
                     LLLIMRDRPVFLSGAFLFTAIMLFTAQFFLKKRFEKKEITWIDAVFIGFFQGIAVLPG
                     VSRSGSTITAALLRKINSEDAVEYSFMLAIPAILGALVLESAKGSFSAIDPVVALSGF
                     AASFLASMIALKFLVFMIRKTMMYPFGIYLGILAVIVLLIF"
     gene            complement(909..>1658)
                     /locus_tag="PL056_14130"
     CDS             complement(909..>1658)
                     /locus_tag="PL056_14130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48200.1"
                     /translation="SVDSDGVSKTDLRYLWDFKTTPGGISLDIVDDSNRAGNPLNNDP
                     SNRISRTAFQAKMKGIYVVRLIVINNKGVSSIPAECTVEAISDDDLAVKLLWDNKNAD
                     IDIHLVRPDGTYGDPVSDCYFWNCSPQYSGARPDWGIEGDGKDDPYLDLDNTTGMGPE
                     TLYVNKPANGTYKVVVHAYDTSKGPSTAVVKAYVHAVEEMSRSLLMTRTDTCWDVYTI
                     DVSDGDGTKKNLVVKEITPAEPYDCARPFQQ"
BASE COUNT          490 a          361 c          329 g          478 t
ORIGIN      
        1 ttaaaacagg aaaagcaagc tttatttgaa tttttatcca tttatttttt tcaccaaagt
       61 tccgctcagg ttgtattaaa ttgtttccag caagtcaagg gaaaacaaaa aaatcttctt
      121 tagaatatta gcagaactat tacagcgagg attccaagat atattccgaa aggatacatc
      181 atcgtctttc ttatcatgaa aaccaggaat ttcagtgcga tcatgctcgc aaggaacgat
      241 gcggcaaaac ctgagagtgc cacaacaggg tctattgctg aaaatgagcc tttcgcagat
      301 tcaagcacta aagcgccgag aatcgcaggg atcgcaagca taaaagaata ttcaactgca
      361 tcttctgagt ttatttttct taaaagtgcg gctgtgatcg tggagccgga acgggaaact
      421 ccgggtagaa ccgcgatgcc ttggaaaaag ccgatgaaaa ctgcatcaat ccatgtgatc
      481 tctttctttt caaacctttt tttaagaaag aactgtgctg taaaaagcat tattgctgta
      541 aaaagaaatg ctcctgaaag aaaaaccgga cgatctctca tgataagcag cagcggtttt
      601 gtaataaaca ccatgccgcc ggttattacc gtgcttatta tcagcagaac cagatactta
      661 tgctgagcat tgtattcttt gaaattagtc attccattaa aaagatctgc tattttcctt
      721 ctgaaaaaga tcattactgc cataagagag gcaacatgga gcagaatgtc ataaatcaga
      781 ggaacatctt tgtgtcccat cagattttct gtcaaggcaa gatgtcctga tgaacttacg
      841 ggaagaaatt ctgtaaaacc ctgaagaagt cccagaataa ttgattcaag tattgtcatc
      901 ggaccggact actgctgaaa aggtcttgca cagtcatatg gctctgcggg agttatctct
      961 tttacgacaa ggttcttttt tgttccgtct ccgtcgctca catctattgt ataaacatcc
     1021 cagcaagtgt cggtacgagt cataagaagc gatcttgaca tctcctctac agcatgaaca
     1081 tacgctttaa ccacagccgt agaaggccct ttggatgtgt catacgcatg aacaaccact
     1141 ttgtatgtgc catttgccgg cttgttcaca taaagcgttt caggccccat tccggttgta
     1201 ttatcaagat caagataagg atcgtccttc ccatctcctt ctatgcccca atccggtctc
     1261 gcacctgaat attgcggaga acaattccag aaataacagt cacttacagg atcaccataa
     1321 gttccgtcag ggcggacaag atgaatgtct atatctgcgt tcttgttatc ccagagcagt
     1381 tttactgcaa gatcgtcatc agatatagct tcaactgtac attcagccgg gatggaactg
     1441 actcctttgt tgttgatgac gataagtctt acaacataga tccctttcat tttcgcctgg
     1501 aaagcagttc tcgaaattct gtttgacgga tcgttgttaa gggggtttcc agccctgttc
     1561 gagtcgtcaa ctatgtctaa tgatattcct cctggagtgg ttttaaaatc ccacagatat
     1621 ctcagatcag tctttgaaac accgtcactg tcaacact
//
LOCUS       DAOOYB010000418         1657 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_96358_length_1657_cov_23.072409, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000418 DAOOYB010000000
VERSION     DAOOYB010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96358_length_1657_cov_23.072409"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1214
                     /locus_tag="PL056_14135"
     CDS             <1..1214
                     /locus_tag="PL056_14135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HPM48201.1"
                     /translation="EKNMAELITGIIIGTITGGIISFLWMKAKNASIVAESKAKLEAM
                     AEKIETQKNEFESMKKQFSAEFENLATKIFDEKSQKFTRLNQENMTTILKPLGDNLEN
                     FKKKVEETYNNEARERFSLEKELKKLVELNLKISDDANNLTNALKGESKTQGNWGEMI
                     LENILEKSGLVKGREYFAQEFLKDADGNPLKNESGQKLQPDVIINYPDNRKIIVDSKT
                     SLTAYVDCVNAGNDEERKMFAKKHLESVKKHIDELSAKRYEDFAPTLDFVMAFIPNES
                     AYILAMQSDPGLWNYAYDRRIILISPTNMIAALKLVADLWKREYQNKNALDIAERGAK
                     LYDKFAGFVENMTDIGLNIQRAQKSYDTALNQLKDGPGNLIGQAEKLRELGVKTKKRL
                     PSSPAEDYKDL"
     gene            1334..1576
                     /locus_tag="PL056_14140"
     CDS             1334..1576
                     /locus_tag="PL056_14140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48202.1"
                     /translation="MFKLASKFFVMAVLLFSFSAMFAAEIHCNPDTKTCHKSTCRYYN
                     CKGCTKVFQSEDEVTKNGYKFCKICSEEKTEKKGKK"
BASE COUNT          587 a          289 c          341 g          440 t
ORIGIN      
        1 aggagaaaaa catggctgag ctgataacag gaataataat tggaacaata actggcggaa
       61 ttatttcatt tttatggatg aaagcaaaaa atgcgtcaat tgttgctgaa tcaaaagcta
      121 aacttgaggc gatggctgaa aagatcgaaa ctcagaaaaa cgagtttgaa tccatgaaaa
      181 agcagttctc tgctgaattt gaaaaccttg cgacaaagat atttgacgaa aaatcgcaga
      241 aattcacgag acttaaccag gaaaacatga cgacaatatt aaaaccgctc ggggataatc
      301 ttgagaattt caaaaagaaa gttgaagaaa cttacaataa tgaagcgcgc gaaagatttt
      361 ctcttgaaaa agagttaaaa aaactggtgg aacttaattt aaagatttca gatgatgcca
      421 acaatctcac aaatgcactc aaaggtgaat caaaaactca gggaaactgg ggtgaaatga
      481 tccttgaaaa cattcttgaa aagtcagggt tagtgaaggg aagagagtat tttgctcagg
      541 aatttctgaa agatgctgac ggaaatcctt taaaaaatga atcggggcag aaacttcagc
      601 ccgatgttat cataaattac cctgacaaca gaaaaataat agtggattca aaaacttccc
      661 tgactgcata tgttgattgt gtaaatgccg gaaatgatga agaaagaaaa atgtttgcga
      721 aaaaacacct ggaatctgta aaaaaacaca ttgatgaact gagtgcaaaa agatacgagg
      781 acttcgcccc gacactcgat tttgtgatgg cgttcattcc gaacgaatcg gcttatatcc
      841 ttgcaatgca gagcgatccg ggactttgga attatgctta tgacagaagg ataattctca
      901 taagccctac gaacatgata gcggcgctga aactggttgc agatctctgg aaaagggaat
      961 atcagaataa aaatgctctt gacatagctg aacggggtgc aaaactttat gataaatttg
     1021 ccggctttgt tgaaaacatg actgacatcg gattaaatat tcagagagct cagaagagtt
     1081 atgacactgc tttaaatcag cttaaagatg gtcccggcaa tctcatcgga caggcggaaa
     1141 aactcagaga actcggggta aaaaccaaaa aacggcttcc ttcatcacct gcggaagatt
     1201 ataaagacct ttaaaccata tctttctgcg catcaacaaa catggcttta tttttccata
     1261 ttttttattg acaattatat tttttgcgct atttttctaa tgcgtgttat tcattttata
     1321 ttaggaggat tcaatgttca agcttgcttc aaagtttttt gtaatggcgg ttcttttgtt
     1381 ttcattctct gctatgttcg cggcagagat ccactgcaac cccgacacca aaacctgtca
     1441 caagtcaact tgcagatact acaactgcaa gggatgtaca aaggttttcc agtccgaaga
     1501 tgaagtgaca aagaacggtt acaagttctg taagatctgc tccgaagaaa agactgaaaa
     1561 gaaaggaaaa aagtaaatcc atttcgcagg tccgcactta gccggcggat ctgctttctt
     1621 tgaaaaaatt aagttctctt ttttgtttgc aatagaa
//
LOCUS       DAOOYB010000419         1636 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_98268_length_1636_cov_5.800759, whole genome shotgun sequence.
ACCESSION   DAOOYB010000419 DAOOYB010000000
VERSION     DAOOYB010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98268_length_1636_cov_5.800759"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1336
                     /locus_tag="PL056_14145"
     CDS             <1..1336
                     /locus_tag="PL056_14145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48203.1"
                     /translation="VLTESDETPDNQTPDENIITPDEEKDDITEEPDEIPDIEYPDLE
                     MNESDSYSGTYGWEIMKDTYYIDPSFDALSFEWDYFMIHNEDDSFSGSVGYLVSNPRN
                     KKIAGNLFGKLVPGGGNVAIAGQFDKTLAPVADYENFDPDNGHTTTISGEERSLWVEN
                     TDKGYWGKMTPNHVDNTMLLEGKTGNFEWELTVRQDWTELTTGSDTFVQVTDNKIDTK
                     VFGIPVAQEWNVHMFWPRTKITGWIKDLRDEKVYDIDAHGYRENSWGRWAFNQGGWDF
                     GTMSDIKSKVMFGWQTYHFNSTRLDYLDLGFIDEGEYKLVNFRADMGQLGWKHDNWSF
                     DPVARQCVPRDSVVVGKNDKYKVVANITIGEGQVPMLSDATDIVKNYVIMIQIPWFEG
                     HIVRLSDNKIIATFSGRGGGEFSTERAKEGTTFISDLDCIFWGSKFSVPIPQ"
BASE COUNT          500 a          315 c          390 g          431 t
ORIGIN      
        1 agttttaaca gagtcggatg aaactcctga caatcaaact cctgacgaaa atatcatcac
       61 tcctgatgaa gaaaaagatg acattacaga agagcccgat gaaattccag acatcgaata
      121 tcctgatctt gagatgaacg aatccgattc atattcaggc acttacggct gggagataat
      181 gaaagacaca tattacatcg accccagttt tgatgcattg tcgtttgagt gggattactt
      241 catgatccat aacgaagatg attccttcag cggttctgtc ggttatcttg tctcaaatcc
      301 gaggaacaag aaaattgcag gaaatctttt cggaaaactt gttccgggtg gcggaaatgt
      361 cgcaattgcc ggacagtttg acaaaactct tgctcctgtt gccgattatg agaattttga
      421 tcccgataat ggtcacacga caacaatttc aggcgaggaa aggtcgctct gggttgaaaa
      481 tacagataaa ggttactggg ggaaaatgac tcccaaccat gttgacaaca ctatgcttct
      541 tgaaggaaag accggaaatt tcgagtggga gcttaccgtc agacaggact ggactgaact
      601 tacgacaggc agtgacacat tcgttcaagt caccgataat aaaatagata ccaaggtttt
      661 cggcatacct gttgctcagg aatggaatgt ccacatgttc tggccgagaa caaagataac
      721 cggctggata aaggatcttc gggacgaaaa agtttatgac attgatgccc atggataccg
      781 tgagaacagt tggggcagat gggcttttaa tcagggcgga tgggatttcg gcacaatgtc
      841 ggatataaaa tcaaaagtaa tgttcggatg gcagacatat cactttaatt cgacgcggct
      901 tgactatctt gatcttggat tcattgatga aggggaatac aaacttgtca atttcagagc
      961 cgatatgggt cagcttggat ggaagcacga caactggtct tttgatcccg tagcccgtca
     1021 gtgcgttccg agagattctg ttgttgttgg aaagaacgat aaatataaag ttgttgcaaa
     1081 cattaccatc ggcgaagggc aggttccgat gctttcagat gccaccgaca tcgttaagaa
     1141 ttatgtgata atgatccaga tcccgtggtt tgaagggcat atagtacggc tttcagacaa
     1201 caagatcata gcaaccttct caggcagagg aggtggcgaa ttctcaaccg aaagggcaaa
     1261 agagggaaca accttcatct ctgaccttga ctgcatattc tggggatcaa aattctcagt
     1321 tcccataccg cagtagaaca agcacacctg ttttttaggg attagctggg atggcttaat
     1381 tgaaaaaggc aaccacccga attcaaaatg aatttaaagc ggaaacaaca gtcttgcgga
     1441 gtgaaagtat atatagatat tatgctccta cccgtatggg tagtttggtt agaataattt
     1501 tacttttcat ctttttatta gggtgtactg acatcaattg ggatctgagt gctgtctgaa
     1561 tctaaaagca tgttataatt ctgcagcccg tgagcgccat aagggacata aagagttggg
     1621 gaattcctgt aaagtc
//
LOCUS       DAOOYB010000420         1585 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_103139_length_1585_cov_3.496732, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000420 DAOOYB010000000
VERSION     DAOOYB010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103139_length_1585_cov_3.496732"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            191..1387
                     /locus_tag="PL056_14150"
     CDS             191..1387
                     /locus_tag="PL056_14150"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HPM48204.1"
                     /translation="MVKPFKADGKKTYNFQVMVNGERIIESTGETNFNKAQKVANKRA
                     LELREGTDYRIQLTRLIRSIKDMPEKAREKAINDCTKSLLSENPVKMEIKSGLSIFLE
                     KPGKAPGARNTLNSFKRVWKALCNWLAEHHPDINYVHELNEEIAENFLNHIWNTGVAE
                     QTYNEYIKILRRIFNVLTKDAGISKNVWNSTEKVKINSVSKKPLTKDEFVSIMQVAEG
                     EIKLLLIIGFYTGLRLGDACQLKWEDVNLKTAMFNITPSKTKGHDKTVNIPIHSILYE
                     ILRRIYSADSEYVLPETAATYLKDPGAVSKKIQKTFVNAGIKTTEERKRGVKNATVYG
                     FHSLRHSFISLCAAEGIPQHVVREMVGHSSDAIHQIYQHANTEQKRKAIDMLPSIEEE
                     NIEKHE"
     gene            1371..1538
                     /locus_tag="PL056_14155"
     CDS             1371..1538
                     /locus_tag="PL056_14155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48205.1"
                     /translation="MKNMNESKEVEENTEYKKKASPEKLIEIAKNFEKIALEENRTHY
                     EIIRAFNSIYT"
BASE COUNT          579 a          275 c          329 g          402 t
ORIGIN      
        1 gggaaaaaat ggtggtcccg acaggagtcg aacctgttgc ctatcactta ggaggaaatt
       61 gcgcacatct tatgtatctg tttttattgc atttttttta cgggttgtaa aaggggttgt
      121 aagtttttct tgacttaact tgtattaact tatatcgtat cctatcacct tctaagatgg
      181 gagttgtgta atggtgaaac cctttaaagc tgacggaaag aaaacttaca attttcaggt
      241 tatggtgaat ggtgagagaa taattgaatc aaccggagaa actaacttca ataaagccca
      301 gaaagttgca aacaagcgtg ccttggaact ccgcgaagga acagattacc ggattcagtt
      361 aacaagactg atcagatcta ttaaggatat gcccgagaaa gccagagaaa aagcaataaa
      421 cgactgcact aaatctctcc tctcggaaaa tcctgtaaaa atggaaataa aatcagggtt
      481 gtccattttt cttgaaaagc cgggaaaagc ccctggtgca agaaacaccc tgaattcatt
      541 taaacgggtt tggaaagctc tttgtaactg gcttgctgaa caccatccgg atataaatta
      601 tgttcatgaa ttaaatgaag agattgcaga aaactttctg aaccatatct ggaacacagg
      661 agttgcagag caaacctaca acgagtatat caaaattcta cgacggatat tcaatgtcct
      721 cacaaaagat gcagggataa gcaagaatgt atggaacagt accgaaaaag ttaaaatcaa
      781 cagtgtcagc aagaaacctc ttactaaaga tgagttcgtt tcaattatgc aagttgctga
      841 aggggaaatc aagcttcttc ttataatagg tttttataca ggtctccggc ttggagatgc
      901 gtgccagctg aaatgggaag atgttaattt gaaaacagcc atgtttaata ttacgcccag
      961 taaaacaaaa ggacatgata aaacagtaaa tattccgata catagcatcc tttatgaaat
     1021 actgagaagg atctattccg ctgacagcga gtatgtgctg ccagaaacag ccgcaactta
     1081 tttgaaagat ccaggggcgg tttcaaagaa aattcagaaa acctttgtta atgcaggaat
     1141 taaaacaact gaagaaagga aaagaggcgt caaaaatgcc actgtttacg ggtttcacag
     1201 cctcagacac tctttcatca gtttatgtgc agctgaagga ataccacagc atgttgtaag
     1261 ggaaatggtc ggacacagct ctgacgcaat tcatcagata tatcagcacg caaatacaga
     1321 acagaaaagg aaagctattg atatgctgcc gagtattgag gaggagaata ttgaaaaaca
     1381 tgaatgagag taaagaagtt gaagaaaata cagaatataa gaagaaagca tcgcctgaaa
     1441 aactgattga aattgcaaaa aactttgaaa aaatcgcctt ggaagaaaac aggactcact
     1501 acgaaattat aagagcattt aactctattt atacctaaat cgttcgattt tcaataaaaa
     1561 acttgacaaa aacctccgaa agtga
//
LOCUS       DAOOYB010000421         1560 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_105560_length_1560_cov_5.193355, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000421 DAOOYB010000000
VERSION     DAOOYB010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105560_length_1560_cov_5.193355"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(42..806)
                     /locus_tag="PL056_14160"
     CDS             complement(42..806)
                     /locus_tag="PL056_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPM48206.1"
                     /translation="MKEGYELVYKRPSTLLDTRMHYCPGCFHSTLHKLIMEVVEEMGI
                     KEDIIGVSPVGCSVFAYDYMDIDFQQAAHGRACAVATGIKRTLPDKFVFTYQGDGDLA
                     AIGTAETIHACNRGEKFSIFFVNNAIYGMTGGQMAPTTLEGQKTTTSPYGRDPKHVGY
                     PLKMAEIISQLPGVAYCERVVLDTPANVRKAKKSIIKCFEIQQQKLGTTFLEVVSNCP
                     SGWKTAPVKTLEFVKDNMLHYYPLGVKKDVTAAENK"
     gene            complement(818..>1560)
                     /locus_tag="PL056_14165"
     CDS             complement(818..>1560)
                     /locus_tag="PL056_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HPM48207.1"
                     /translation="LGTIQPSQGDYFQAVKGGGHGDYRLIVLAPNSVQEMVDHTVMAF
                     DLADKWRNPVLILTDGAIGQMMEKIELPDPYPLRSAEDMRDEKPWATNGRKGGVKKNL
                     LTSLNIKSDEMERKNNELQAKYGKIENEEVRYESYNLDDADIVISAYGLSSRICKKVV
                     DLGREQGLKIGLLRPITLWPFPSKKLDELAAKKNVKMFMTIEMSVGQMVEDVRLVVNG
                     RKPVYFYGRTGGILATAEEILDQIKMKM"
BASE COUNT          405 a          364 c          295 g          496 t
ORIGIN      
        1 ctgcaaaaat catttcagca agcatattat tttcctccta gttatttgtt ttcagcagcg
       61 gtaacatctt ttttaacacc gagcggataa taatgaagca tattatcctt aacgaattca
      121 agtgttttta caggagccgt tttccagccc gaaggacagt ttgaaacaac ttcaagaaat
      181 gtcgttccca gtttctgctg ctgaatttca aaacatttta tgatcgattt ctttgctttt
      241 ctgacatttg ccggagtgtc gagaacaact ctttcacaat atgcaacacc cggaagctga
      301 ctgattattt cagccatttt aaggggatat ccgacatgtt taggatctct tccgtaaggg
      361 ctggtagttg ttttctgacc ttcaagtgtg gttggagcca tctggccacc ggtcataccg
      421 taaatagcgt tatttacaaa gaaaatcgaa aatttctcgc cacggttgca ggcatggata
      481 gtttcagcag ttccgatcgc cgcaaggtca ccgtctccct gataagtgaa aacaaatttg
      541 tcaggaagag ttctttttat acctgttgca acagcacatg ctcttccgtg agccgcctgc
      601 tggaaatcta tgtccatgta atcataggcg aaaaccgaac atccgacagg gcttacaccg
      661 attatgtcct ctttgatccc catttcttca accacttcca tgatcagttt gtgaagagtg
      721 gagtgaaaac atccggggca gtaatgcatt ctggtatcaa gaagcgtcga cggtctttta
      781 taaactaatt catatccttc tttcatttta tgcctcctta cattttcatt ttgatctgat
      841 caaggatctc ttcagccgta gcaagaattc cccctgttct gccataaaaa taaacaggtt
      901 tgcgaccgtt tacgacaagc cgtacatctt caaccatctg accgacactc atctcgatcg
      961 tcatgaacat ttttacattc ttttttgccg caagttcatc aagttttttt gaggggaaag
     1021 gccagagcgt tatgggcctg agaagaccga tcttgagacc ctgttctctt ccaagatcaa
     1081 cgactttctt acatattctg cttgaaaggc cataggcgct tataactata tcggcatcat
     1141 caagattata tgattcgtaa cgaacttctt cattctcaat ttttccatat tttgcctgaa
     1201 gttcgttgtt ctttctttcc atttcatctg actttatatt aagcgatgta agaagatttt
     1261 tcttaacacc gccttttctt ccgtttgtgg cccatggctt ttcatctctc atatcttcag
     1321 cacttctcaa aggataggga tcggggagct ctattttttc catcatctgg cctattgctc
     1381 cgtcagtaag aattagaacc ggattgcgcc atttgtcggc aagatcgaat gccatgacag
     1441 tgtggtcaac catttcctga actgaattgg gagcaagaac aataagccgg tagtcaccat
     1501 gcccaccgcc tttaactgcc tgaaaataat cgccctgact cggctgaatt gttccaagac
//
LOCUS       DAOOYB010000422         1536 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_108066_length_1536_cov_7.990547, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000422 DAOOYB010000000
VERSION     DAOOYB010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108066_length_1536_cov_7.990547"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1536)
                     /locus_tag="PL056_14170"
     CDS             complement(<1..>1536)
                     /locus_tag="PL056_14170"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HPM48208.1"
                     /translation="FADMDNDGDLDALNVVYVDKSSYTTDGYMDDRTKVYLNNGDGTF
                     KPGPFYDFGPSMYEAFAGISFFDYDLDGILDMFAGRHYNDYGVLDSCEQDNLYKGLGD
                     GKFKEMTKGSGVETQKVSDETLASGKNHKATWGVASCDVDGDGYTDIMTTTYGRSFNM
                     FYRNKGDGTFEDLSISSNFASDGIEDYSDDQFFRCFCADAANKDTEYCKDAPSPSITC
                     GSYSWTPGFSDQPHRLGGNSSGTLCADFDGDGDMDMFAIELAHWHIGSASDKSMLIMN
                     DGFPEKPFRRVDEKESGITRARTGGWNDGDLGGLAADFDNDGKMDIYILSSDYDGTKA
                     LLFQQQQDGKFKETGKDAGAQIPRAHGGALVDIDRDGDYDLIVGVSMMRWGTDTADYN
                     KRPEKQWVTVLRNDTGADANKVIISITGSKANRSAIGARIIVKAGGKEFIREVQSSYG
                     LTGFQNDSLQIIGLGNICEIEEIEVRWPDKDRTVTKYTNVYANYLLEIDQEKGLKHYT
                     LED"
BASE COUNT          381 a          393 c          279 g          483 t
ORIGIN      
        1 taatcttcca aagtatagtg tttaagtcct ttttcctgat cgatctcaag aagataattt
       61 gcatacacat ttgtatattt tgtaacggtt ctgtctttgt cgggccagcg gacttcaatt
      121 tcttctattt cgcagatatt tccaagtcct attatctgga gagagtcatt ctggaaacct
      181 gtaagtccgt aagaactttg aacttctctg atgaattcct tcccgcctgc tttcacgatt
      241 atccttgctc ctattgccga tctgttcgct tttgagccgg taatactgat aatgactttg
      301 ttggcatcgg cccctgtatc atttctcagc actgtaaccc actgtttttc aggtcttttg
      361 ttataatcag ccgtatctgt tccccacctc atcattgaaa caccgactat cagatcataa
      421 tcgccatccc tgtcaatatc gaccaatgct ccgccgtgag ctctcggtat ctgtgctccg
      481 gcatcttttc ctgtttcttt aaattttcca tcttgctgct gctgaaaaag aagtgctttt
      541 gttccgtcat agtcagaact taaaatataa atgtccattt tcccgtcatt atcaaaatct
      601 gccgccagtc cgccaagatc tccgtcattc cagccgcctg ttcttgccct tgttattccg
      661 ctttccttct cgtcaacccg tctgaaaggt ttttcaggaa atccgtcatt cataattaac
      721 attgacttgt cacttgcaga accgatatgc cagtgggcaa gctcgatcgc aaacatgtcc
      781 atgtctccgt caccgtcaaa atcggcacac aaagttcctg aagaatttcc gccgagccgg
      841 tgcggctgat ctgaaaaacc gggcgtccac gaataacttc cgcaggttat tgaaggactt
      901 ggtgcgtctt tgcagtactc agtatcctta tttgccgcat cagcacagaa acatctgaaa
      961 aactggtcat cgctgtaatc ctcaataccg tcacttgcaa aatttgaaga aatggacagg
     1021 tcttcaaaag tgccgtcacc tttattgcgg tagaacatat tgaacgacct gccgtaagta
     1081 gtcgtcatga tgtctgtata tccgtcaccg tcaacatcgc aagaggcaac tccccatgtc
     1141 gctttatgat tttttcctga tgcaagagtt tcatcactca ctttctgtgt ttcgacaccg
     1201 cttccttttg tcatctcttt gaactttccg tcaccaagtc ctttgtagag attatcctgt
     1261 tcgcacgaat caagcacacc gtaatcgtta taatgtcttc ctgcaaacat atcgagaatc
     1321 ccgtcaagat cgtaatcaaa gaatgatatt ccggcaaatg cctcatacat cgaaggaccg
     1381 aaatcgtaaa atggtccggg cttgaaagtg ccgtcaccgt tgttgagata tactttcgtc
     1441 ctgtcatcca tatagccgtc agttgtataa gatgatttat caacatatac tacattcagg
     1501 gcatccagat caccgtcgtt atccatgtcg gcaaaa
//
LOCUS       DAOOYB010000423         1520 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_109809_length_1520_cov_12.558362, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000423 DAOOYB010000000
VERSION     DAOOYB010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109809_length_1520_cov_12.558362"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(218..1459)
                     /locus_tag="PL056_14175"
     CDS             complement(218..1459)
                     /locus_tag="PL056_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HPM48209.1"
                     /translation="MVTVRPFKAVRPRDEIAHKVASFPYDVLNSKEARELAKGDEYTF
                     LHVNKPEIDLPEDIDLYDESVYQKGKENFYGMIEKGIFVQDGTPHLYIYRQTMNGHEQ
                     YGIVGCASADEYNNDLIKKHEFTRKKKEDDRTKHVITLNANAGPVFLTYRDNAKIDAL
                     VAEVVKNKPEVDFVATDGIGHTVWVIKDERINAEISSTFRNDVPVLYVADGHHRSASA
                     ARAKAERLKTDPNPSDEKEYNYFLSVFFPGTQLKIFDYNRVLLTMNGLTKDEFMAKML
                     EKFEIVRTGEDRPKMATEFGFYMDGEWKIIKAKAGTYPSDDPVEGLDVSILQNNLLAP
                     VLGIGDPRSDENIDFVGGIRGTIELKKRVDSGECTMAFSLFPTSVEQLMSVADAGKIM
                     PPKSTWFEPKLRSGLLVHLLD"
BASE COUNT          419 a          327 c          298 g          476 t
ORIGIN      
        1 cattatcaat gcagaccccg tcaatgcatg aaaaaccggg cagacagtca aaattgttaa
       61 tacaccccgg ttcatcttca acagtgcagg aaataaagag ggttagcaga aataatgatg
      121 aaattaaaag cttgaagttc atattaatct cctgaaaaaa aactttgaaa atatatcaga
      181 aaagtgtgca aattacaagg tgaagggaaa attgatgtca gtcaagaaga tggacaagga
      241 gtccgcttct gagtttcggt tcaaaccatg ttgattttgg cggcatgatc ttgccggcat
      301 cggcaacgct catgagctgt tcaactgatg ttgggaaaag tgaaaaagcc attgtgcact
      361 caccgctgtc aactcttttt ttaagttcaa tcgttcctct gattccgccg acaaaatcga
      421 tgttttcatc gcttctgggg tcgccgattc cgagaacggg tgcaagaaga ttgttctgca
      481 gaattgatac atcgagccct tcaacagggt cgtcacttgg gtaagtgcct gcttttgctt
      541 tgattatctt ccattcgcca tccatataga aaccaaactc agttgccatt ttgggtctgt
      601 cttcgccggt tcttactatt tcaaatttct caagcatttt tgccatgaac tcatcttttg
      661 tgagtccgtt catagtgaga agaactctgt tgtaatcaaa tattttaagc tgtgttccgg
      721 gaaagaaaac agaaaggaaa tagttgtatt ccttctcgtc agacgggttc ggatctgttt
      781 tcaatctttc cgcttttgct cttgccgcac ttgctgatct gtggtgaccg tcagcaacat
      841 acagaactgg aacatcattt ctgaaagtgc ttgatatttc agcgtttatt ctttcatctt
      901 tgatcaccca gactgtgtgt ccgatcccgt ctgtggcgac aaaatcaact tctggcttat
      961 ttttaacaac ctcagccaca agtgcgtcaa tcttcgcatt atctctgtat gtaagaaaga
     1021 caggacctgc gtttgcattg agcgtgatga catgttttgt tctgtcgtcc tcttttttct
     1081 ttcttgtgaa ctcgtgtttt tttataaggt cgttgttgta ttcatcagca cttgcacaac
     1141 cgacgattcc atattgttcg tgaccgttca tagtctggcg atagatataa agatgaggtg
     1201 ttccatcctg aacgaatatc cccttttcga tcataccgta gaaattttcc tttcccttct
     1261 gataaacgct ttcatcataa agatcgatat cttccggaag atcaatttcc ggcttgttca
     1321 catgaagaaa agtgtactca tctccctttg caagctctct tgcctcttta gaattaagaa
     1381 catcataagg aaaactcgca accttgtgag cgatctcgtc acgcggtctt acagctttaa
     1441 aaggcctgac agtaaccatg gtctcctcct gaaaaaaagt ataaaaatac aaacaacaaa
     1501 gacttttaat atttgcaatg
//
LOCUS       DAOOYB010000424         1504 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_111564_length_1504_cov_9.137336, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000424 DAOOYB010000000
VERSION     DAOOYB010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111564_length_1504_cov_9.137336"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            93..>1504
                     /locus_tag="PL056_14180"
     CDS             93..>1504
                     /locus_tag="PL056_14180"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPM48210.1"
                     /translation="MKRFYLGFIGFSQKHTFLMLIFMTGLIAFELIYLKNNLKIDTDL
                     KALFKGNNPTVLELQDMEERVGTYSTVLVVANSPDREKNIEALLRIKNKINDDPAVRF
                     IEFDRDIDYIEKHALLFASVEELEKIRNEVRNAIADKVAESMSLSDNSDTSEKSGGTI
                     SLTEKLDEIEKKGRIYRDKYNISRFYEAENGTFIAMKVRPGGSETNVSDTKTIVNLLD
                     KAIIETKPEELGVTVEAGGHFRNKLKEMNAIYNDVFSTLAICIFLLCLTIIFYFRSLR
                     ALLIVFLPLSAGVLSAITAMQFFTGGFNIISAFSFTILYGLGIDFSIHLLGRFSEESE
                     TAGNCRTALENSSTGILPAIFSGALTTVAAFFSLILIDFKGFSDFGLAAAIGVTTSFI
                     SILIFFPAVIFAFDRVKPLKIKAYKTEFLAAVYLKISKRPATVVTVMGIVTICSIFSI
                     KFSEMEYNFDKLTFPGKYDRS"
BASE COUNT          469 a          290 c          300 g          445 t
ORIGIN      
        1 atgcaacctt gcatatacga attaacttta ctgataaaac tgatttgtta atatcagaaa
       61 gtattgtttt gataaactcg ggagcagagg atttgaaaag gttttatctt gggttcatag
      121 ggttttcaca aaaacacact tttctgatgc ttatatttat gaccggtctt attgcctttg
      181 aacttattta cttaaaaaac aatcttaaaa tcgataccga cctgaaagca cttttcaaag
      241 ggaacaaccc tacagtatta gagcttcagg acatggaaga aagggtcggg acttattcta
      301 cggtgcttgt tgttgcaaac tctcctgaca gggaaaagaa tattgaagca cttctgagaa
      361 taaagaataa aattaatgac gaccccgctg tcaggttcat cgaatttgac agggatattg
      421 attacattga aaaacacgcc ctcctttttg catctgttga agagcttgaa aaaataagaa
      481 atgaagtcag aaacgcgatt gcagacaaag ttgcggaatc gatgtcgctt tcagacaaca
      541 gcgatacttc tgaaaaatcg ggtggaacga tctctttgac agaaaaactt gatgaaattg
      601 agaaaaaagg acggatttac agagataagt acaacatcag ccgtttctat gaagcggaaa
      661 acgggacttt tatcgcaatg aaagttcgcc ccggtggctc ggaaaccaat gtaagtgaca
      721 caaaaacgat cgtgaacctg cttgacaaag caattattga aacaaaaccg gaagaacttg
      781 gtgtaacagt cgaggcaggc ggtcatttca ggaacaaact taaggaaatg aacgctatat
      841 acaacgatgt cttttcaacc cttgcgatct gtatttttct gctgtgcctg acgatcattt
      901 tctatttcag atcacttagg gcacttctta tcgtttttct tcccctttca gcaggggttc
      961 tttcggcaat aacggcaatg cagttcttta caggcggatt taacataatt tcggcttttt
     1021 cattcacgat cctttacgga ctcggaattg atttcagcat acatcttctc gggagattca
     1081 gtgaagaaag cgaaacagcc ggaaattgca gaactgcact tgaaaattcc tccaccggaa
     1141 tactgcctgc gatattttca ggagctttaa ctaccgttgc agcattcttt tctttaatac
     1201 tcattgattt taaaggtttt tccgatttcg gtcttgccgc ggcaataggc gttacaacat
     1261 cattcatttc gatacttata ttctttccgg cagtaatttt tgcttttgac agggttaagc
     1321 ctcttaaaat aaaggcctat aaaactgaat ttctggcggc ggtatatttg aaaatatcaa
     1381 aaagaccagc taccgtagta accgttatgg gaatagttac gatctgctcc attttttcaa
     1441 taaaattcag cgagatggaa tacaatttcg ataagctgac tttccccggc aaatatgaca
     1501 ggtc
//
LOCUS       DAOOYB010000425         1502 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_111808_length_1502_cov_8.724948, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000425 DAOOYB010000000
VERSION     DAOOYB010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111808_length_1502_cov_8.724948"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1412)
                     /locus_tag="PL056_14185"
     CDS             complement(<1..1412)
                     /locus_tag="PL056_14185"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPM48211.1"
                     /translation="MKKLYLKFIGFSQENTLFMMILMIVLITFELVYLKNNLKIDTDL
                     KALFKGHNPTVLELQDMEERVGTYSTVLVVANSPDREKNIEALLRIKNKIIDDPAVRF
                     IEFDRDIDYIEKHALLFASVEELEKIRNEVRNAIADKVAESMSLSDNSDTSEKSGGTI
                     SLTEKLDEIEKKGRIYRDKYNISRFYEAENGTFIAMKVRPGGSETNVSDTKTIVNLLD
                     KAIIETKPEELGVTVEAGGHFRNKLKEMNAIYNDVFSTLAICIFLLCLTIIFYFRSLR
                     ALLIVFLPLSAGVLSAITAMQFFTGGFNIISAFSFTILYGLGIDFSIHLLGRFSEESE
                     TAGNCRTALENSSTGILPAIFSGALTTVAAFFSLILIDFKGFSDFGLAAAIGVTTSFI
                     SIIIFFPAVIFAFDRVKPLKIKPYKTKFLATVYSKISKRPAAVAAIMGIVTICSIFSI
                     KFGEMEYNFDKLTFPGKYDRS"
BASE COUNT          434 a          298 c          296 g          474 t
ORIGIN      
        1 gacctgtcat atttgccggg gaaagtcagc ttatcgaaat tgtattccat ctcgccgaat
       61 tttattgaga aaatggagca gatagtaact attcccataa tagctgctac tgcagccggt
      121 ctttttgata ttttcgaata tactgtcgcc aggaatttag ttttgtacgg ctttatcttc
      181 aggggcttaa ccctgtcaaa cgcaaaaatt actgccggaa agaatataat tatcgaaatg
      241 aatgatgttg taacgccgat tgcggcggca aggccgaaat cggaaaaacc tttaaagtca
      301 ataagtatta aagaaaagaa tgccgcaaca gtagttaaag ctcctgaaaa tatcgcaggc
      361 agtattccgg tggaggaatt ttcaagtgca gttctgcaat ttccggctgt ttcgctttct
      421 tcactgaatc tcccgagaag atgtatgctg aaatcaattc cgagtccgta aaggatcgtg
      481 aatgaaaaag ccgaaattat gttaaatccg cctgtaaaga actgcattgc cgttattgcc
      541 gaaagaaccc ctgctgaaag gggaagaaaa acgataagaa gtgccctaag tgatctgaaa
      601 tagaaaatga tcgtcaggca cagcagaaaa atacagatcg caagggttga aaagacatcg
      661 ttgtatatag cgttcatttc cttaagtttg ttcctgaaat gaccgcctgc ctcgactgtt
      721 acaccaagtt cttccggttt tgtttcaata attgctttgt caagcaggtt cacgatcgtt
      781 tttgtgtcac ttacattggt ttccgagcca ccggggcgaa ctttcattgc gataaaagtc
      841 ccgttttccg cttcatagaa acggctgatg ttgtacttat ctctgtaaat ccgtcctttt
      901 ttctcaattt catcaagttt ttctgtcaaa gagatcgttc cacccgattt ttcagaagta
      961 tcgctgttgt ctgaaagcga catcgattcc gcaactttgt ctgcaatcgc gtttctgact
     1021 tcatttctta ttttttcaag ctcttcaaca gatgcaaaaa ggagggcgtg tttttcaatg
     1081 taatcaatat ccctgtcaaa ttcgatgaat cttacggctg gatcgtcaat tattttattc
     1141 tttattctca gaagcgcttc aatattcttt tccctgtcag gagagtttgc aacaacaagc
     1201 actgtagaat aagtgccgac cctttcttcc atgtcctgaa gctccagcac tgtcggattg
     1261 tgccctttga aaagtgcttt gagatcggta tctattttaa gattgttctt taaataaacg
     1321 agttcaaatg ttatcagaac gatcataagt atcatcataa acagagtgtt ttcttgtgaa
     1381 aaccctatga atttaagata aagctttttc aaggtagtct cccggtgttt caaaacttca
     1441 gactttagat attaacaaat cagttttatc agtaaagtta attcgtatat gcaaggttgc
     1501 at
//
LOCUS       DAOOYB010000426         1482 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_114159_length_1482_cov_9.328662, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000426 DAOOYB010000000
VERSION     DAOOYB010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114159_length_1482_cov_9.328662"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            71..391
                     /locus_tag="PL056_14190"
     CDS             71..391
                     /locus_tag="PL056_14190"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HPM48212.1"
                     /translation="MNKKIFLLTILMLFASDISALDPSKKLHHYIHRSWQAIDGLPQN
                     SANSIVQTDDGYIWIATQEGLTRFDGLNFTVFSRATHPEILSNDLRSLFKGPDGTIWA
                     GTKG"
     gene            451..>1482
                     /locus_tag="PL056_14195"
     CDS             451..>1482
                     /locus_tag="PL056_14195"
                     /inference="COORDINATES: protein motif:HMM:NF019136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HPM48213.1"
                     /translation="MNGLYFTDGRSSRVTDTSGGLSSNTVYSLYIDEDGNIWTGTDSG
                     LNIISGGVVYRVKNIDFLYHDSIYSIAPDGNGNIWLGSNKGLFKASLKEVLEKTLKND
                     NSFEFKRYSYADGMKSMECNGGFTPSYSFHDNLLYFPTLNGISVVDLKIDILNRTVPN
                     LIIEKIFSEDTSGDLHNGNPVVFKPGSRKFEIKYTAPSFVNPQAIKFKYRLFGFDNDL
                     VDAGNRRTAYYTNLKPGDYTFFVTASNNEGIWNKEGEKLDFSITPYFYQTWPFYAFII
                     ILSVFSISLPIMGYSRKKIRTIEIEKIALEIQAIQGIRVTAEIPVTRVIQETPIIRMN
                     AQMNAKTQHA"
BASE COUNT          477 a          301 c          300 g          404 t
ORIGIN      
        1 gcaaatattt gcaaaatcat ttgtttttat gtttaatttc atggtgttga tttaactgaa
       61 ggtgaaacgg gtgaataaaa agatatttct gctcacaatt ctgatgctgt tcgcatctga
      121 tataagtgct cttgatcctt caaaaaaact tcaccactac atccacagat catggcaggc
      181 gatcgacggt cttccccaga actctgcaaa ctcgatcgtc cagactgacg acggatatat
      241 ctggatcgcc acacaggaag ggctcacgcg atttgacgga cttaacttca cagttttcag
      301 cagagctaca caccccgaaa tattgagcaa cgacctcaga tcgcttttca agggacctga
      361 cggaactatc tgggcaggaa cgaagggata aacgacggca ttattctgac atttcatcct
      421 gacagatcag gaaatttatg gataggtact atgaacgggc tttattttac tgacggaaga
      481 tcttcaaggg tgactgacac atcaggagga ctttcaagca atactgtata ctccctttac
      541 atagatgagg acggcaatat ctggacagga acagattcag gactgaacat cataagtggc
      601 ggagttgtgt atagagttaa aaacattgac tttttatatc acgattcaat ttacagcatt
      661 gctcctgacg ggaatggtaa tatctggctt ggcagcaaca aggggctttt taaagcatca
      721 ttaaaggaag tccttgaaaa aacactgaaa aacgacaaca gttttgagtt caaacgatat
      781 agctacgcag acggaatgaa aagtatggaa tgtaacggcg gcttcacacc ttcttattct
      841 tttcacgaca atctgcttta ttttccgaca cttaacggaa tttctgttgt tgatctcaaa
      901 attgacatac ttaacaggac tgtcccgaat ttgataattg aaaagatctt ttcggaggat
      961 acctccggag accttcacaa tgggaaccct gtcgttttca aacccggttc aagaaagttt
     1021 gagataaaat ataccgctcc gagctttgtc aatccccagg ccataaagtt caaataccgt
     1081 cttttcggat ttgacaacga ccttgttgat gccggcaaca gaagaactgc atattacaca
     1141 aacctaaagc caggagatta cacttttttt gtaactgctt caaataacga gggaatctgg
     1201 aataaagaag gagaaaaact tgatttttcc ataactccat acttctatca gacatggccg
     1261 ttctatgctt tcatcatcat tctatcagta ttttcaatat ctttacctat tatgggttat
     1321 tcaaggaaaa agataagaac tatagaaatt gaaaagatcg cacttgaaat ccaggcaatt
     1381 caggggatac gggtaacagc ggaaattccg gtaactcggg taattcagga aacaccgata
     1441 atcaggatga atgcacaaat gaatgcaaag acgcaacatg cg
//
LOCUS       DAOOYB010000427         1451 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_117937_length_1451_cov_10.871060, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000427 DAOOYB010000000
VERSION     DAOOYB010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117937_length_1451_cov_10.871060"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            4..>1451
                     /locus_tag="PL056_14200"
     CDS             4..>1451
                     /locus_tag="PL056_14200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012900136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HPM48214.1"
                     /translation="MTDSKQIQHQEILERIARKVMVERGLEPDFPREAVSQLNRILAP
                     ASLEVDNVKDLRHLLWCSIDNDDSLDLDQLTAATLLPDGSVKVFVAIADVDAIVKNYT
                     AIDLHARFNTLSVYTAAIIFPMLPEKLSNNLTSLNKNEERLAVVVEMVIPDDGSLKSS
                     AIYRAAVENHAKLCYNDVSAWLEETAPAPKELSAVKGLEESIKLQDKVAQKLRSHRYE
                     NGALDLETIEGRPVFEGSVLKDYRIEKGGRADQIIEDFMIAANGIAARFLASKKFPSI
                     RRIVRVPKNWERIVALAGEQNFTLPAKPDSKALEKFLIKAKMDDPVRFPDLSLSVIKL
                     LGPGEYVVELPGETSTGHFGLAVKDYAHSTAPNRRYPDLITQRLIKAAIADQQPPYSK
                     KELDDIAKNCTKQEDAAKKVERQVTKSAAALLLGSKIGEDFEAIVTGASEKGTWVRLL
                     ELPFEGMLSAGFEGLEVGERLTVRLLKTDVERG"
BASE COUNT          436 a          307 c          352 g          356 t
ORIGIN      
        1 attatgacag acagcaaaca gattcagcat caggagattt tggagcgtat tgcaagaaaa
       61 gttatggttg aaaggggtct tgagcccgat tttccgcgtg aggcggtttc gcaactgaac
      121 aggatacttg ctcctgcttc tcttgaagtt gacaatgtga aagatctcag acatctgctc
      181 tggtgttcga ttgataacga tgattcgctt gatctggacc agcttactgc cgcaactctt
      241 ctgccggacg ggtcggtgaa ggtttttgtc gcaattgctg atgttgatgc aattgtaaaa
      301 aattacacag cgatagatct tcatgcaagg ttcaatactc tttcggtcta tactgctgcg
      361 atcatatttc caatgcttcc tgaaaagttg tccaacaatc tgacttcatt gaataaaaac
      421 gaagaacggc ttgcagttgt cgttgaaatg gtgatcccgg atgacggttc tttaaaaagt
      481 tccgccattt accgtgcggc agttgaaaac catgcaaagc tttgctacaa cgatgtttct
      541 gcgtggcttg aagaaaccgc tcctgcacca aaagaactga gtgctgtgaa aggacttgaa
      601 gaaagcatca aacttcagga taaagttgca cagaaactga ggtcacaccg ttatgaaaac
      661 ggagcgctcg atcttgagac aattgaagga agacccgttt ttgaagggag tgttcttaaa
      721 gattacagaa ttgaaaaagg gggtcgtgca gatcagataa ttgaagactt catgatcgcg
      781 gcaaacggta tcgctgcaag atttcttgcc tcaaagaaat tcccgtcgat acgccgtatt
      841 gtaagagttc ccaaaaactg ggagaggatc gttgcccttg caggcgagca gaatttcacc
      901 cttcctgcaa aacccgattc aaaagcactt gaaaaatttc tcataaaagc aaagatggac
      961 gatccggtaa gatttccaga cctttctttg agtgttatca agctgttagg tccaggagaa
     1021 tatgttgttg aactgccagg ggaaacttcg acaggacact tcggacttgc agtaaaggat
     1081 tatgcccatt ccacagcacc caaccggaga tatcccgatc tcataactca gcggctgata
     1141 aaggcggcaa ttgcagatca acagccgcca tattcaaaaa aagaacttga tgacattgct
     1201 aaaaactgca caaaacagga agatgccgca aagaaagttg aaagacaggt gacaaaatcg
     1261 gccgccgcac ttctgctcgg ctcaaagatc ggtgaggatt ttgaagcaat agtcacaggc
     1321 gcatccgaaa aagggacatg ggtgcggctt cttgaacttc cgtttgaagg aatgctctca
     1381 gccggctttg aaggactcga agtaggcgaa cggctcacag tcagactcct gaaaaccgat
     1441 gtggaaagag g
//
LOCUS       DAOOYB010000428         1362 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_129997_length_1362_cov_5.003826, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000428 DAOOYB010000000
VERSION     DAOOYB010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129997_length_1362_cov_5.003826"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..1235
                     /locus_tag="PL056_14205"
     CDS             60..1235
                     /locus_tag="PL056_14205"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPM48215.1"
                     /translation="MKNSYRELFGIENGFTALNHGSFGACPVEIINYQFELVRKMESL
                     TTRFFTRIIKPLIAESMEILSSFVNAPANDIVFVRNATTAANAVINSMDFKKGDEIVT
                     TNLIYGSCRNALDHATETKGAVVKKALIPFPVRSENEIAESILGLITKKTKLIFIDHV
                     TSETAMIMPVKKICVEANRLGIDIFIDGAHAPGMIPLDISAINPTYYTGNCHKWICAP
                     KGTAFLYVRPDRQKSVIPPIISNHFQEGTTPNEKLHNSFDWSGTMDYTNYACVGKTIE
                     YLRDSVPGGWKGIMQRNHELAVTGRNIVVKKLHLDQYLPDELIGSMATIKLNSTAVID
                     PSTGLDVIQMKLLDEYKIEALITTLYPTGERILRISAALYNNENDYELLEEALKKTV"
BASE COUNT          451 a          276 c          277 g          358 t
ORIGIN      
        1 tacagaataa agtaaataag taaattaccg gctgcagcaa tgttattgga ggtgtttaaa
       61 tgaaaaacag ttacagggaa cttttcggga ttgaaaatgg cttcaccgct ttaaatcatg
      121 gttcattcgg tgcatgtccc gttgagatca tcaattatca gtttgaactt gtaagaaaaa
      181 tggaatcgct aaccacgcgg tttttcaccc gcattatcaa gccgctgata gccgaatcca
      241 tggaaatatt aagcagtttt gtcaatgcac ccgccaatga catagttttt gtgagaaacg
      301 cgacaactgc ggcaaatgct gtgataaatt caatggattt taaaaagggt gatgaaattg
      361 taacgacaaa tctgatctat ggttcgtgca gaaacgccct tgatcatgcg acagaaacaa
      421 aaggtgctgt ggttaaaaaa gcgctcatcc ccttccctgt cagaagtgaa aacgagatcg
      481 ctgaatcaat tcttggactt atcacaaaaa agacaaagct gatatttatc gaccatgtca
      541 caagcgaaac tgcaatgatc atgccggtta aaaaaatctg tgttgaagca aaccggctcg
      601 ggatcgatat tttcattgac ggagcgcatg ctccagggat gattccgctt gatatttcag
      661 caataaatcc gacatattac actggcaact gccacaaatg gatatgcgct cccaaaggca
      721 ctgcgtttct ttatgtcaga ccagacaggc agaaatcggt aattccgccg atcatcagca
      781 atcactttca ggaagggaca acaccaaacg aaaaactgca taattcattt gactggtcag
      841 gaactatgga ttatacaaat tacgcctgcg tcggaaaaac catcgaatat cttagagata
      901 gtgttcccgg cggctggaaa ggaatcatgc aaagaaatca tgaacttgca gtaacaggaa
      961 gaaacatcgt cgtaaaaaaa cttcaccttg accagtatct gccggatgaa ctcatcggct
     1021 caatggcgac tatcaaactc aattcaaccg cagttataga tccctcgaca ggacttgatg
     1081 tcatacagat gaaacttctt gatgaatata agatcgaagc actcatcaca acactttatc
     1141 cgacaggcga aaggatcttg agaataagtg ccgccctcta caacaacgaa aatgattatg
     1201 aactgcttga ggaagcgctg aaaaagacgg tttagtcctg caattcatca aaatgacact
     1261 caagtctaat tttgttgctt tatagtttct ttacttatat gatacctcgt gttgtgaatc
     1321 ttttgaagga gtatttatta tgaacatgaa tatgaacttt at
//
LOCUS       DAOOYB010000429         1351 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_131599_length_1351_cov_7.022377, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000429 DAOOYB010000000
VERSION     DAOOYB010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131599_length_1351_cov_7.022377"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1336)
                     /locus_tag="PL056_14210"
     CDS             complement(<1..1336)
                     /locus_tag="PL056_14210"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48216.1"
                     /translation="MKNGCSESQIGQIIKGQYLGYFTYRVDITGFFNRIYEMNAELER
                     PEDGKYYGTYVFSDLDCTEHDYYRCGNKMLSSWAIFIVYRSQHIRPKRVYFYNGLAFI
                     HGDKSVASISGFELRRNPSIHLTTMIAGGDIKPVELNLPEEGILLQGEGATSRFRLYN
                     ECNPLMGSYVEVFNSVSSIANWSLDATENIKCVSNFGIDVDTFVLDSDDINLQEHLKK
                     GNTSMEITLSVNQDEIFTNFMVLSANQTAEGPIFDIPPEASDPAKSKYNFPLDREKHF
                     CACPSTDGGRVDDYYCETVNSRREFFYFIKVQNWGEDDAEDVVVSDELDKSLEYIQGS
                     TEMATQYSPELDAYTDWKSIPDKPDGVFPLSGAGYKVAAKMRPCDQPNWTCQDTVFIR
                     YKVKPKAGIEKGSIFRNTALIKDKNSDEPYKTNLSYPLKLNPTTCVADTTCPT"
BASE COUNT          376 a          291 c          269 g          415 t
ORIGIN      
        1 gagtcgggca tgttgtgtcg gctacacagg tcgtgggatt aagtttaagc ggatatgaca
       61 ggttggtttt atacggctca tcgctgtttt tgtctttgat aagagccgta ttccggaata
      121 tagagccctt ttcaattcct gctttcggtt tcactttgta tcttatgaaa actgtatcct
      181 gacatgtcca gttgggctga tcgcacggac gcattttggc ggcaacttta taacccgccc
      241 cggaaagagg aaaaactcca tcaggtttgt caggaattga tttccagtcc gtataggcat
      301 cgagttcagg agaatactgt gtcgccattt cagtagatcc ctgaatatat tcaagacttt
      361 tatcaagttc atcactcacc acaacatctt ccgcgtcgtc ttcaccccag ttctggactt
      421 tgataaaata aaagaactct cttctgctgt taacagtttc gcagtagtaa tcatcaactc
      481 ttccgccatc agttgatgga caagcacaga aatgtttttc gcggtcaagg gggaaattat
      541 actttgattt tgccgggtcg cttgcttcag gcggaatatc gaaaattgga ccttctgctg
      601 tctggttagc tgaaagaacc atgaaattag tgaatatctc atcctgattg actgaaagag
      661 taatttccat tgaagtgtta ccttttttca aatgctcctg aagattgatg tcatcactat
      721 caagaacgaa tgtatctaca tcaataccga aattggaaac acatttaatg ttttctgtcg
      781 catcaagact ccagtttgcg atcgatgaaa cggaattaaa aacctcgaca tagcttccca
      841 taagaggatt gcattcgttg taaagacgga acctgctcgt ggcaccttca ccttgaagta
      901 aaattccttc ttccggaaga tttaattcta caggtttgat atctccacct gcgatcatcg
      961 ttgtaagatg gatggacggg tttctccgga gttcaaaacc gctgattgat gcaactgatt
     1021 tgtcaccatg gatgaaagca agaccgttgt agaaatatac ccgtttcggt ctgatatgct
     1081 gtgaacggta aacaatgaaa atcgcccatg aacttaacat tttattgccg caccggtagt
     1141 aatcgtgctc agtacagtca agatcactga aaacatatgt tccgtaatac ttcccgtctt
     1201 caggtctttc cagttccgcg ttcatttcat aaattctgtt aaaaaaacca gtaatatcaa
     1261 cacgatatgt gaaatatccg agatattgtc ccttaataat ttgaccgatc tgcgattcac
     1321 tgcatccatt tttcacatca tcctgaaaat a
//
LOCUS       DAOOYB010000430         1349 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_131953_length_1349_cov_3.420402, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000430 DAOOYB010000000
VERSION     DAOOYB010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131953_length_1349_cov_3.420402"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="PL056_14215"
     CDS             <1..315
                     /locus_tag="PL056_14215"
                     /inference="COORDINATES: protein motif:HMM:NF017013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOFRL family protein"
                     /protein_id="HPM48217.1"
                     /translation="KVKGEGRGGRCSELMLALSIELQFMPGVSALCFASDGHDGNAPS
                     LGAFADHTSYQRLISQHNGISPENYLKRNDTFTAFTVIGDSLSEQNTGTNMNDVLIVL
                     FS"
     gene            696..>1349
                     /locus_tag="PL056_14220"
     CDS             696..>1349
                     /locus_tag="PL056_14220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HPM48218.1"
                     /translation="MASLDCSRYYNDLKEMSSVRAMAETLMNNSKVRKITAAEAYELA
                     KKQAGVSETDYEIYPPYAQQLGLPIGAKVLNDCHGNIIGRTAKARRFYNKLNQGDKDK
                     VEGDVREALFQMEHYPLIKAEAVLGQDPNLMIKATFVTTESDAMNVFNWLCNFTPIEV
                     FEQQYKNSKEVHIIDIILVAFNEWTCNDPFYNNVGAPQLALVDELNNVIFNFGMRYFG
                     "
BASE COUNT          461 a          192 c          289 g          407 t
ORIGIN      
        1 aaagtaaagg gagaggggag agggggcaga tgctcagaat tgatgttagc attaagcatt
       61 gaacttcaat ttatgcctgg tgtctcagcc ctttgttttg cttcagatgg gcatgatgga
      121 aatgcacctt cgttaggagc ttttgcagat cacacttctt atcaaagatt gatatctcaa
      181 cataatggca tatctccgga aaactattta aaaagaaatg acacttttac cgcatttaca
      241 gttataggtg attcattgtc tgaacaaaat acaggaacga atatgaatga tgttttaatc
      301 gttttattca gttaaaaagt gataaaatca aaagaattgc tataaaattg aaaatctgtt
      361 catttttggc tgtattcgaa tagaatttat aacttattga taaatagtaa aaaagaataa
      421 tctgattaag gacaaataaa ttatttataa atttttcttg acaaagaaat cggtatctgt
      481 taattcttga ctcataagat gaaagcttca cctgcgggtg gagttctttt ttctttttat
      541 agtgtgtata cgagatagga gaaaatagca cctggttttc tctccttcaa ctgaaggaga
      601 gtcaattgaa ggtatgttga aattacaaac aaaaaagttt gtcgtatgga gaaatattcc
      661 aaataaaaaa tagagtatgt cttgtgggag gaacaatggc aagtcttgat tgtagtcgtt
      721 actacaacga tctgaaagaa atgtccagtg taagagcgat ggctgagaca ctgatgaaca
      781 attctaaagt gagaaaaatc actgcagctg aagcttatga attagcaaaa aaacaggcag
      841 gtgtaagcga aacggattat gaaatttatc cgccttatgc gcaacagctt ggtttaccaa
      901 taggtgcaaa ggtactaaat gattgtcatg gaaacatcat tggtcgcaca gcgaaagccc
      961 gccgttttta caataagctg aatcagggag ataaggataa ggttgagggt gatgtaaggg
     1021 aagcattatt tcaaatggaa cattatccgt taataaaggc tgaggcagta ttaggacaag
     1081 atccaaatct gatgatcaaa gcgacttttg taacgactga atctgatgca atgaatgtgt
     1141 ttaactggtt gtgtaacttc acaccgattg aagtgtttga acaacagtat aaaaacagta
     1201 aagaagtgca tatcattgat attattctgg ttgccttcaa cgaatggact tgcaacgatc
     1261 ctttctataa taatgtggga gcacctcagt tggcattggt agatgaactg aataatgtga
     1321 tttttaactt tggtatgcgt tacttcggg
//
LOCUS       DAOOYB010000431         1311 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_137872_length_1311_cov_5.374204, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000431 DAOOYB010000000
VERSION     DAOOYB010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137872_length_1311_cov_5.374204"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1168
                     /locus_tag="PL056_14225"
     CDS             <1..1168
                     /locus_tag="PL056_14225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48219.1"
                     /translation="KYDCCGYPCEIKGIEPVYWDSNVSKCDIDPQPLVDGQYWTGGSG
                     RRHWSNISDGKLGFIMSAWKPDYMVVSQYRAFEYDMNNKSYKVFGPASLGHSYHKGSV
                     FTTTFDFNKINNVDDVQPGISNYWYYYSNGKYQVAYDKPSTAPSYPTMNEKWVAASFG
                     PLDKSETSGYRYAKIGEWKWQQLNESLSWGQVQGDTLIFGTWEQEVFWCDLSKNPKST
                     SDCIKVNRSGESGVYAIMDTENAKRGAFTDYGHNIMYIVTLGDDGKLSYESVIPEKTE
                     ETHVGFSTSYLTGDMLLYGEGFAPNGGNEGKMCFYNLKTKKQYCSKPVIKNKDGFLTY
                     YQGDAMFEGKWMAWQNFSSGMMIRDMECYCKENPEICPFDDYLPKTLKSKDISK"
BASE COUNT          446 a          212 c          309 g          344 t
ORIGIN      
        1 caaatacgac tgttgcggat acccttgtga aataaaaggt attgaacctg tatattggga
       61 ctcaaatgtc agcaaatgtg atatagatcc gcaacctctt gttgatggac aatactggac
      121 aggaggatcg ggcagaagac actggtcaaa tatttctgat ggaaagcttg ggtttattat
      181 gagtgcatgg aagccggatt atatggtcgt tagtcagtac agagcatttg agtatgatat
      241 gaacaataag tcctataagg ttttcggacc ggcatctctg ggacattcct atcataaagg
      301 atctgttttt acaaccacgt tcgacttcaa taaaattaac aatgttgatg atgttcaacc
      361 gggaatttca aattactggt attattacag caatggtaaa tatcaggttg cttatgataa
      421 accctcgact gcaccaagtt atccaacgat gaacgaaaaa tgggtggcgg cgagcttcgg
      481 acctctggat aagagcgaaa catccggcta ccgttatgca aagataggtg aatggaagtg
      541 gcagcagttg aatgaatcat taagctgggg acaggttcag ggagacacac tcattttcgg
      601 gacatgggaa caggaagttt tctggtgtga tctgtcaaag aatccgaaat ccacctcaga
      661 ttgcataaaa gtgaacagaa gtggagagtc aggtgtgtat gcaataatgg atacagaaaa
      721 tgcaaaaaga ggagcattta ccgattacgg acacaacata atgtatattg tcacactcgg
      781 agatgacgga aaactgtcgt atgaatctgt tattcctgaa aagaccgaag agacacatgt
      841 gggattcagc acatcatatt tgacaggaga tatgctgtta tacggcgaag gattcgcacc
      901 caatggcggc aacgaaggga aaatgtgttt ttataatctc aaaacaaaaa aacaatattg
      961 ttcaaaacct gtaataaaaa acaaagatgg atttctgaca tattatcagg gcgatgcgat
     1021 gtttgaagga aaatggatgg catggcagaa tttcagttca gggatgatga tccgcgacat
     1081 ggaatgctac tgcaaggaaa atccggaaat ctgcccgttt gatgattatt tgcctaaaac
     1141 gcttaaatcc aaagatattt caaaataaaa ttgatacttt gcaattcaac ttgtgatgga
     1201 tacaatgaaa gtgtttgtga aacggatgga tagtaatttt ttgaagggta aaataaatta
     1261 tgacagacag caaacagatt cagcatcagg agattttgga gcgtattgca a
//
LOCUS       DAOOYB010000432         1295 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_140516_length_1295_cov_11.815323, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000432 DAOOYB010000000
VERSION     DAOOYB010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1295
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140516_length_1295_cov_11.815323"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            137..937
                     /locus_tag="PL056_14230"
     CDS             137..937
                     /locus_tag="PL056_14230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48220.1"
                     /translation="MKLFKTVLTVAVTFFFFHAFAGVKIVTESVEHSNNEKSSGTLMI
                     DGDLVRVETSESGEQSVIYDLKKKEVILINHKEKNWMKMTKKQIDDSKAHVKAQMKAA
                     VAQQQAALAQLAPEQRAQLETQMQMMLGNENKTPLNYVKTGKRSKWGDSECEIYDGMR
                     GDIKTEEICTVVPKKLKCSLVEIERLKQISLDYAMDENDVSAWKDVKNLGIPVIHKMI
                     SNGKVVVTNTLVSFENTKIPAESFKVPADYKQTSSPLDSVVPAPEKKK"
     gene            complement(992..1207)
                     /gene="rpsU"
                     /locus_tag="PL056_14235"
     CDS             complement(992..1207)
                     /gene="rpsU"
                     /locus_tag="PL056_14235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HPM48221.1"
                     /translation="MRRQKVIAPLEVYVTDDLEKAIKILKKKMALEGLYKELKKRRHY
                     EPPSVRNRLKKEEAERRRRKNMKKKRV"
BASE COUNT          430 a          254 c          270 g          341 t
ORIGIN      
        1 tgttctgtca aacattgtcc ctcctccggg tcgtccggat attataaccg caaaatggtg
       61 aaaaaagtaa aaattgtttt tcaaactccc taaattataa ttgcgcagac actttgaaat
      121 ttgggaggga acagaaatga aattatttaa aacagtttta acagttgcgg tcaccttttt
      181 cttttttcat gcttttgcgg gagttaagat cgtgactgaa agtgttgaac actcaaacaa
      241 tgaaaaaagt tcaggcactt tgatgatcga cggagatctt gtcagggtgg aaacttcgga
      301 atcgggcgaa cagtccgtaa tttacgatct taagaaaaaa gaggtgatac tgataaatca
      361 caaggaaaag aactggatga aaatgacaaa aaaacagata gatgattcca aagcccatgt
      421 taaagcacag atgaaagcgg ctgtcgctca gcagcaggcg gctcttgcac agcttgctcc
      481 ggaacagaga gcacagcttg agacacagat gcagatgatg ctgggaaatg aaaataaaac
      541 acctctgaac tatgttaaaa caggaaaaag atcaaaatgg ggtgattcgg aatgtgaaat
      601 atatgacgga atgaggggcg atatcaagac agaagagatc tgcacggttg tcccgaaaaa
      661 acttaaatgc tcccttgtcg agatcgaacg gcttaaacag atatcacttg attatgctat
      721 ggatgaaaat gatgtttctg catggaaaga tgtaaagaat ttaggtattc ccgttatcca
      781 caaaatgatc agcaatggaa aggttgtggt taccaacaca cttgtgtctt ttgaaaacac
      841 aaagatcccg gcagaaagtt tcaaagtccc tgcagattac aaacagactt cctcaccgct
      901 cgattcggta gttcctgcac ctgaaaagaa aaaataaaag acaaaaaaaa agaccgcccg
      961 aaggcggccg gcaaatcaga acagaaaaga gctaaactct cttcttcttc atgttcttcc
     1021 tgcgtcttct ctcggcttct tcctttttaa gacggttacg cactgatggt ggttcgtagt
     1081 gacgacgctt cttaagttcc ttataaagac cttcaagagc cattttcttc tttaagatct
     1141 ttattgcctt ctcaagatca tcagtaacgt aaacctctaa aggagcaata actttctgcc
     1201 tgcgcattct ttttcaccgc ctttcatact tcggatttgt tcaaacaaca tgaagcgaga
     1261 ttatcttatg aaaaaacaac aggttgtcaa gtcgg
//
LOCUS       DAOOYB010000433         1283 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_142554_length_1283_cov_5.358306, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000433 DAOOYB010000000
VERSION     DAOOYB010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142554_length_1283_cov_5.358306"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="PL056_14240"
     CDS             complement(<1..544)
                     /locus_tag="PL056_14240"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPM48222.1"
                     /translation="MKLVIRNGKLINPAGNINGKTALIIEDGIITEITENPDISGAEV
                     IDAEGMIVAPGFIDIHVHFREPGYEHKEDILTGLNAAAAGGFTAVCTMPNTNPVPDNA
                     ETVKLMLEKAAKGNGVKLYPVASATKGIKGIEMSEPDELKKAGAVALSDDGRPVADTA
                     LLKKIMEKGRSCGMKYVSHSE"
     gene            complement(546..902)
                     /locus_tag="PL056_14245"
     CDS             complement(546..902)
                     /locus_tag="PL056_14245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48223.1"
                     /translation="MSTGKIIQIVIAGLIIYVGWFVAKPYVDKYRLEKVVENIAQYGT
                     INTIERTEKEFQNRIVDLGRKDILPGMLFLEKDEETQRVKATLRYKDEIKVFGHVFKP
                     LEFTIIKEAAKVDKII"
     gene            complement(983..>1283)
                     /locus_tag="PL056_14250"
     CDS             complement(983..>1283)
                     /locus_tag="PL056_14250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM48224.1"
                     /translation="GCSAEVKILGEYVAVKNDTKLFDLLKKISSEKGIRCIEKKGELV
                     GEDFGYFTQKWGGLLFWLGSKDPDKNPNPLHSEKFFPSFKAIDTGLEIMTEILLY"
BASE COUNT          324 a          307 c          235 g          417 t
ORIGIN      
        1 cttcactgtg ggaaacatat ttcatgccgc aggatctccc tttttccatg atctttttca
       61 ggagagctgt gtcagcaaca ggtcttccgt catcactgag cgcaaccgct cccgcttttt
      121 taagttcatc cggctcagac atctcgattc ctttgatccc ttttgtcgcc gaggcaactg
      181 gataaagctt gacaccattc ccttttgcgg ccttttcaag catcagtttt acagtttcag
      241 cattgtccgg aacaggattt gtgttcggca tagtgcacac agccgtgaaa ccgcctgccg
      301 ccgccgcatt gagccctgtc agaatatctt ccttgtgctc atatcccggt tctctgaaat
      361 ggacatggat gtcaatgaaa ccgggagcaa cgatcatccc ttccgcatct atgacttccg
      421 cccctgaaat atcggggttt tcagttattt cagtaattat gccgtcttca attatcagcg
      481 cagtttttcc gttgatattt ccggcagggt tgatcagttt tccgtttctg ataacaagtt
      541 tcattttata ttatcttgtc aactttggca gcttctttga ttattgtgaa ttcaagcggt
      601 ttgaaaacat ggccgaaaac cttgatctca tccttatacc tgagagttgc tttaaccctc
      661 tgtgtctctt catccttttc aagaaaaagc atgccgggga gaatatcctt tcttccaaga
      721 tcgacaattc tgttctgaaa ttctttctct gtcctttcaa tcgtgtttat cgtgccgtac
      781 tgggcgatgt tctctacaac tttctcaaga cggtatttat caacatacgg cttagccaca
      841 aaccagccga cataaattat aagacctgca attacaatct gaatgatctt tcctgtactc
      901 atgaattttt ctcctgaaaa aagttttgaa cgataaccgg tgaaaagtat cagataactt
      961 aaattaagtc aaaaaaaaca ggtcagtaaa gaaggatttc tgtcattatc tcaagtccgg
     1021 tgtctatcgc tttgaaactt gggaaaaact tctctgaatg caaggggttg ggatttttat
     1081 caggatcttt tgaaccgagc cagaaaagga gtccgcccca tttctgagtg aaatatccga
     1141 aatcttcacc tacaagttca ccttttttct caatgcatct gatccctttt tctgaagaga
     1201 tctttttcag cagatcaaaa agcttagtgt cgttctttac agctacatat tctccaagta
     1261 tcttcacttc agcactgcat cca
//
LOCUS       DAOOYB010000434         1249 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3574
            NODE_148665_length_1249_cov_9.091290, whole genome shotgun
            sequence.
ACCESSION   DAOOYB010000434 DAOOYB010000000
VERSION     DAOOYB010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865309
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,851
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_148665_length_1249_cov_9.091290"
                     /isolate="AMR_MDS_3574"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..>1249
                     /locus_tag="PL056_14255"
     CDS             90..>1249
                     /locus_tag="PL056_14255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HPM48225.1"
                     /translation="MTVVINGGKLTVEELVRVARFGEKVELAPEALERIKICRAMLER
                     KIENHEIMYGVNTGIGEFSETVLDDSDIKDFQKYLIYNHSAGIGEPAPLEYVRGAILA
                     RINVHAHGNSGCRPEITQTFVELLNKNVIPFVCQKGSVGACGDLAPMSQIALTLLGEG
                     MAYYKGELLPAKEAYDKAGIPVPGLHARDGLAAINGSNVLTAMSALLIYDVERWLKQA
                     EIATAMSLVALKANLKPYLPDIHVARGFSGAIRSAASILKLVEGCDLLTGKVKVKVQD
                     AYSMRSTPQVIGAAHDAVKWAKSQVETELSGVCDNPVFFPEKNMALSGANFQGTPVAL
                     PMEIVGQAVTMVSVLSERRMNRLNNPALSVGLPPFLATNPGLFSGLMLSQYTA"
BASE COUNT          341 a          257 c          330 g          321 t
ORIGIN      
        1 cggcggaagt ttaaattaaa tttggatttt ttttcaagtg tgctaaattg ctttcgcgat
       61 attttttcta actttttttg gagagcacaa tgacagttgt tatcaatggc ggaaagctta
      121 cagttgaaga gcttgtaagg gttgcgaggt ttggtgaaaa agttgagctt gcccccgagg
      181 cgcttgaaag gatcaagatt tgcagagcta tgctcgaaag aaagatcgag aatcacgaga
      241 tcatgtacgg agtgaacaca gggatcggcg aatttagcga aaccgtgctt gatgacagtg
      301 atatcaagga cttccagaaa tatctgatct ataatcattc agcaggaata ggagagccgg
      361 cgccgcttga atatgtgaga ggggcaatac ttgcaaggat aaatgttcac gcccatggaa
      421 acagcggctg tcgtcctgag ataacgcaga cttttgttga gcttttaaat aagaatgtaa
      481 tcccttttgt atgtcagaaa ggatcagtcg gcgcctgcgg agaccttgct ccgatgagcc
      541 agatcgcact cactctgctt ggagaaggga tggcatatta taaaggtgaa ctccttcctg
      601 caaaagaggc gtatgataag gcgggaattc cggttcccgg ccttcatgca agagacggac
      661 ttgccgcgat aaacggttcg aatgtactca cggcaatgag cgcactttta atttatgatg
      721 ttgaaagatg gcttaaacag gcggaaattg caactgcgat gtcgctcgtt gcgttaaagg
      781 caaacctcaa accgtatctt cccgatattc atgtcgccag gggcttttca ggagcgataa
      841 gaagtgctgc gtcaatattg aaacttgtcg aagggtgcga ccttctcacc ggaaaagtga
      901 aagtaaaagt tcaggatgca tattcaatgc gttcaactcc gcaggtgatc ggtgccgccc
      961 atgatgcggt aaagtgggcg aagtcacagg tggaaactga gctttcagga gtctgtgaca
     1021 acccagtttt cttccctgaa aagaacatgg cattgagcgg agcgaacttt cagggaacac
     1081 ctgttgctct tccgatggaa atagtgggtc aggcagtcac aatggtaagc gttttgagcg
     1141 aaagaaggat gaaccggctt aataatcccg ccctttcagt cggactcccg ccttttcttg
     1201 ccacgaaccc cggtcttttc agcgggttga tgctttctca gtacacagc
//
